LOCUS       DAOMYG010000001        34312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_465_length_34312_cov_10.136177, whole genome shotgun sequence.
ACCESSION   DAOMYG010000001 DAOMYG010000000
VERSION     DAOMYG010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 34312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34312
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_465_length_34312_cov_10.136177"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PK610_00005"
     CDS             complement(<1..475)
                     /locus_tag="PK610_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRJ34332.1"
                     /translation="MKRIILFSSAILMFAFSATKIDPSSVDGNKRKVPAVKVKTLDGG
                     TFNMADVANGGKPIVISFWATWCSPCKKELENMAEVYEDWQKETGVKIIAISIDDARN
                     ASKVKPYIVSKGLPYDVYIDENQDFKRAMNVNNVPHTFLVDGEGHIIWDHNNYSEG"
     gene            complement(616..1317)
                     /locus_tag="PK610_00010"
     CDS             complement(616..1317)
                     /locus_tag="PK610_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ34333.1"
                     /translation="MRISLTIFGCLFIPLFGITQVFSVDTTYKPIVKTTASITPPHEH
                     FQLFNHTGDSLNMRWRIDPADTYYPSQWSIAIQDNITYHNPAPDSSDFILPPIAGTMD
                     KIIINIFHNAYPGFGEVHIDLFTTDYPADTIRIIFDIRIMQALGTDESYLVMLKTWPN
                     PSAGSFEVSFPSLLDAEAEYSIIGVDGRQVVRGVILPGIQSQDFNLTGLAKGSYYLKI
                     STKDVVYQSVCLVVE"
     gene            complement(1388..3322)
                     /locus_tag="PK610_00015"
     CDS             complement(1388..3322)
                     /locus_tag="PK610_00015"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HRJ34334.1"
                     /translation="MKKLLLSVAAVIGAFPVMAQTVIFSENFQSVTTPAMPAGWIMTN
                     VDGLTPATNVNWCTNAWVTRADFASASNTVAASISWYTPAGVSNDWMATPQITLPSGT
                     GLTLRWRSMAQDPSYPDGYRVYVTTASGGAPADFTGGSGAQLLNIPADATTWTWRQIN
                     LDAYAGQTIRLGFQNYSNDMFVLMLDDIQVLQLNPNDASVTSITTNPFVVGPANVNIT
                     GAIYNAGATPITAVTLKWSDGTTTHNQNLTGLNIASGASYNYSHSASLAVAAGTNYNI
                     KVWTELAGDPNTANDTAYATIYGLNFLPTKRVLGEEGTGTWCGWCPRGHVFMAQMEQN
                     YPNTWVGVAVHNSDPMTVTAYDAAIGGLIGGYPSGVVDRDALDIDPSQFPATYNQMIT
                     KVPPANISVSNTWNAGSRAMTVTVNANWAGSFNNLNFRLGAILIENNVTGTTSGYNQT
                     NYYSSTSQNIALTGAGHNWQTSPNPIPAAQMEYDHVGRALLGGFAGQTGSVPTSVTAG
                     SNTNYTFNHTLPAGQDENDIEIIGVLIDNATGKILNAAKAHMTVGIEEDGVDNFDFSI
                     FPNPANDMTTVKLNIQEAGEVSISIISITGAIVSSFNYGTLVGENNFVIDANRLESGI
                     YFVNVNVGGNMITRKVVVTH"
     gene            3656..4420
                     /locus_tag="PK610_00020"
     CDS             3656..4420
                     /locus_tag="PK610_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ34335.1"
                     /translation="MKRQLLALLFAFGFLAVNAQSFQFHDQSGNNLSGQTVTLTGDDS
                     EYELTDYIKLMNNSGTSKNIKIKRYEVSVTPATTNYFCWTVCYAPMTAGAYPQFPYVG
                     HLAWNDYVTASGSAYAPYQLIVYHKPNGNAGNSLYRYVAFDGANPNDSVEVFVAVNIS
                     AANSVNELFAVKSLNLFPNPANQVANIQYQFNNAAGEQQLVVSDMIGNRRKVMQLIGT
                     EGTVRLDLNEYASGIYFVTVMRNGEAVSTKRLVVTH"
     gene            complement(4417..5121)
                     /locus_tag="PK610_00025"
     CDS             complement(4417..5121)
                     /locus_tag="PK610_00025"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013752069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRJ34336.1"
                     /translation="MKKKKLVVFSGAGISAESGLRTFRDSDGLWEEYNVMDVATPEAW
                     VKDQELVLEFYNKRRAQILEAKPNLAHISLVELEKAYDVTVITQNIDDLHERAGSRHV
                     VHLHGNIRYARSTNPKDQHLYPISGDNLNFGDKCPAGFQLRPHVVWFGEEVPLLPVAQ
                     KIVEGADVLIITGTSLTVYPAANLVRYAGFRCKKFLVDPHTIHLNDIDGLEIIKEKAS
                     SGIPKLAQQLLLEPVG"
     gene            5224..7503
                     /locus_tag="PK610_00030"
     CDS             5224..7503
                     /locus_tag="PK610_00030"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRJ34337.1"
                     /translation="MTQIKFKKQDALDYHSSGRKGKIEVIPTKPYASQRDLSLAYSPG
                     VAEPCLAINKRKDDVYKYTAKGNLVAVISNGTAVLGLGDIGPEASKPVMEGKGMLFKI
                     YADIDVFDIEIDETDVDAFVKVVKAIAPTFGGINLEDIKAPECFEIERRLKEELDIPI
                     MHDDQHGTAIISGAALLNALELAGKKISKVKIVVNGAGAAAISCAKLYVSLGAKKENI
                     LMVDSKGPITKNRKDLDEHKSFFASARKVNSLEEAMEDADVFLGLSKGNILSKEMVKS
                     MAKNPIVFALANPDPEISYKEAKTAREDVIVATGRSDQPNQVNNVLGFPYIFRGALDV
                     RATKINEEMKLAAVRAIAELAKEPVPEEVNEAYGEKNISFGRENIIPKPLDPRLLWTV
                     APAVAKAAVDSGVAKEPMKDIDAYIEELQKKTGHANKLLRSISEKASKNPKRIVFAEG
                     DNYKILKAAQIASDEGIATPILLGDAKKIRGLIEEYSLDLEDAEIIDPKDASEYERRV
                     KYGELLFKKRQRKGMTLFEAQKAMRERNHFGAMMVEVGEADALISGISRSYKEVIRPS
                     LQIIGTADDVERVAGMYVIFTKDGPVFFADTTVNHSPNAEEIVDITLMVAKAVRNFRI
                     TPRIAMLSYSNFGSANGDDPDKMRKAVSILHKEHPNLIVDGEMQANFALNQDLLQEFF
                     PFSDLNGSKPNTFIFPNLASGNIAYKMIQEMTNAEVIGPILLGMRKSFHVLQIGCSVR
                     EIVNMVKIAVVDAQTKSKK"
     gene            7504..8076
                     /gene="ruvA"
                     /locus_tag="PK610_00035"
     CDS             7504..8076
                     /gene="ruvA"
                     /locus_tag="PK610_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010179761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRJ34338.1"
                     /translation="MIEFIKGRLTEKNPAYVVIEANGIGYLLHISLNTFSQIGKDENV
                     SLYTHQVIREDAHLLFGFSTKEERSVFRSLLSVSGVGASTARMVLSSLGPDEVQQAIA
                     MSNVSAFQKIKGIGAKTAQRIIVDLSGKIEKIELAGNQPGGVLRQEALGAMVTLGFDK
                     AQAEKAVDKVLATDNNLPVEQLIKLALKNL"
     gene            8092..15426
                     /gene="sprA"
                     /locus_tag="PK610_00040"
     CDS             8092..15426
                     /gene="sprA"
                     /locus_tag="PK610_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRJ34339.1"
                     /translation="MSATTTIRKTVLTIGSSLIGLFLIAFANTVFKGNIVSRFVGPPV
                     ADSPTVNLPYPIYPPNSSNSGNSSNVNLQNPLNFSDSVVYDPTNGTYNFNYQVGNSIP
                     YQPSSSMTLEEYLDYDMEKALQKGWQDLSEEYGKKPGGADIPFLKPKAEGGMFGSDFI
                     DIKPSGTAELRFGINTSKTENPQIPEKQRKITVFDFDQRIQLNVVGTIGNKLKLNTSY
                     NTQATFDFENQMKLEYKGDEDEIIQSIEAGNVQLPLTGSLITGSRSLFGIKTQLKFGK
                     LTATTVFSQQKGKRTEVEVSGGAQVSNFELTADNYEANKHYFTSHFFRNTFDNAMAAL
                     PQVASQVNITRMEVWVTNTNNTVDNTRNIIAFADLGETSIFEGAPNAINGTLPRNEAN
                     GIYAFASANPNIRGFNNATAALNAVVTSPGPFQQAVHYEKVQNARKLTDQEFQFNPML
                     GFVTLNQPLNNDEVLAVAFQYTYQGQTYQVGEFSTDGVAGQDALVLKLLKGTVTNPIY
                     KRWDLMMKNIYSLGAYQVNRDNFRLDIWYNNPATSVDINFIPQPGVDNIPIIQLLNLD
                     KFDPNNNPFPDGVFDFMPLTFSGNKANGGGTINATNGRVIFTTVEPFGGTLRNKLLVA
                     GVDPTVVNTIVYQPLYDSTKTAAQQIPQLNRFKIKGRYQSSTSSEISLNALNIPQGAV
                     TVTAGGRTLVENSDYTVDYNLGRVRIINTGLLESQTPIRISVESNTLFSMLTKTMLGT
                     RFDYRLSKDASLGATVLNMTERPLTQKINIGDEPVSNTMLGFDGSFKKEVPYLTRLVD
                     KLPLISTKEKSMLSASAEYATIIPGTARAINGISYIDDFEGSQSAIDIRSFSQWQLAS
                     VPKGQPDLFPEGSLINNLDYGKNRALFNWHVVDPLFYNNNNLTPQHIKNSPMQSNHTM
                     RQIFEQEVFPNKQLAQGQPTNIAMFDIAFWPSERGPYNYDTSPSAYSAGLSGTGLLNA
                     PDSRWGGIMRQLQTTDFELANIEFIQFWLMDPFNIDATTDLGVTGGDLYFNLGNVSED
                     ILSDSRKSFENGLPISTNFDPNTLDTTVWGLVATTQPIVNAFENSSDPSIRLRQDIGL
                     DGLNSQTEQAFFQSFYNWIGANVTDFNARTQIQNDPSNDDYNYYRDDRYDASQLNILQ
                     RYKRYNGQEGNSPTNEFSQTLNSDGYPTSASTIPNVEDINQDNNLSEAESYFQYKISL
                     RPQDMVIGQNFITDKLTKTVTLPDGSTKQITWYQFKVPIRKYEKAVNGISDFRSIRFI
                     RMFVKNAQNPLILRFARLELIRGEWRTYIPSLLDPGEYIQGDPNSTTFNLFAVNIEEN
                     GNRVPTNYVLPPGINRQVDVATANLRNLNEQSLAFEVCGLEDGDGRACYRNVAFDVRS
                     YKRLKMFVHAETTSLTEQVTNNKDVTLFVRLGTDFTENFYEVELPLIMTPWGATVDTD
                     IWPSANELDIDFEVLRNLKLERNSRVLAGLAANNQLFIRNDPGYPDRLIKIIGNPNLQ
                     GLKVVMIGVRNPNKNTNPWDASDNGTAKCFEVWINELRLTDFDQQGGWAAMGRVNANF
                     ADFAQVALSGNYSTPYWGSIEKKVSERQRETRYGWDGSSSFELGKFFPEKWGIRIPMF
                     LGYSQTIIKPQFDPLNPDIPFNDLPSDQQAISKQKVIDYTQRKSINFSNVRKERTGGK
                     KAHFYDIENWSATYSFSETYKRDINTEYNISKSIRGGLNYNFNATPKSWEPFKSNKLI
                     GKSKWFTIIKDINISKGPKLVAFRNDLNRTYNAYQIRNNFDGIVFPTYNKNFIWVRAY
                     DFKYDLSKNIKFDFGANNNAVVGEPLGRVDRQYRDEYQVFKDSVMQSLRSFGINKMYN
                     HTGNVNITWPINKIPALDWITLTTRYSASYEWQRAPFGQDTLGNTVQNSRNVNWNAQL
                     NFNTLYNKVPYFKKVNQKLQGSGGAKPGGKPSTNSKETPKEGKDGKTSSDSTKKEKKE
                     PLFTVGENIARVVMSVKTLSLTYSTTDGIMMPGFGQTNSVLGMDNQWNSPGLGFVAGK
                     QNKDLFGRESDWGNGYNDYAMYAADQGWLVKNQFLNAQYTANHTKNLSARTSLEPLTG
                     LRIELNADKNQTENYNTFFRWFDTLNGAPFFDYTAQNPMTQGMLSMSMNTWKTAFIGN
                     GENNSSPVFSNFRDIRSEASQLFASGNPNSSLLSSSETGYAGGYGSTQQDVMITSFIA
                     AYSGKKVDQIGSNPFALRPAPNWRVTFDPMAKSKIKFLKKWFKSFSISHGYRSTMTIS
                     NYTTNLLGTRDTQGNMDATDISGNFINPRQISMITISEQFSPLINIDMTWNLKNRGKP
                     GGLITKVELKRDRNLSLSLVNNQVTEVRGAEFVIGSGYRFNNLKLPFNIGGKPTESDL
                     NCRIDLSVRNNVTYTRKVVENQNQATAGQRIVSIKSSADYAISSKLTIRFFYDKVLNR
                     PVISTSFPTSNTNSGIAIRFTL"
     gene            15464..15841
                     /gene="gcvH"
                     /locus_tag="PK610_00045"
     CDS             15464..15841
                     /gene="gcvH"
                     /locus_tag="PK610_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRJ34340.1"
                     /translation="MNIPANLKYTKDHEWIRIEGDFAIVGITDFAQGELGDIVYVEIE
                     TSGETLSKEEVFGTVEAVKTVSDLFMPVSGEITEVNPALEGNPESVNKDPYGEGWMIK
                     VKLSNPSELDALLSDTQYKELVG"
     gene            15845..16231
                     /locus_tag="PK610_00050"
     CDS             15845..16231
                     /locus_tag="PK610_00050"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRJ34341.1"
                     /translation="MLLRFLFPAICWALLIFLLCIAPSDQLPDTSIWDFISFDKVAHL
                     FIFSVFSLLIIVGLRRQYSYSALRYHAKRVAFLAASVYGVIIEFIQQVMNSGRHFEWM
                     DVVADVIGSLLGITLFRIIYGKELAR"
     gene            16292..16966
                     /locus_tag="PK610_00055"
     CDS             16292..16966
                     /locus_tag="PK610_00055"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRJ34342.1"
                     /translation="MEIKKNPDVDINKKLPSLKMMGLLVALAFTLTAFEYTVFEKEEL
                     ALNYDTTNDDLLDEQIVDIPPPPPPPAPPPPPMIVTEIKTVEDDKKVDNVQTFQETDV
                     NDKIDIVDVKVEEAPVVEEIFDVVEEQAEFPGGMEKLRDFLSANLKFPPMAKESGVQG
                     KVYVQFVVFKDGSVGDIKVLRGIGSGCDEEAVRVVKSMPKWNPGKQRGRAVSSRFTLP
                     INFKLQ"
     gene            complement(17011..17568)
                     /locus_tag="PK610_00060"
     CDS             complement(17011..17568)
                     /locus_tag="PK610_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34343.1"
                     /translation="MFDTISKAFDQKPGLYFNWGSQYCFISNRLATMSNIRVGIDYGG
                     SFKLGIGFNWLNKKFPEQDRFIQGMERSSQLKYYYFSVYGEYSFFRNYHWEATIPAQI
                     GAGWARYEFVGGRSTNKAFVVYEPMMTIQYRFWRYFGVGCGIGYRIGIINRTAIPEQF
                     FSPTLILKSKLFVGDLWRDITKKGK"
     gene            complement(17684..19483)
                     /locus_tag="PK610_00065"
     CDS             complement(17684..19483)
                     /locus_tag="PK610_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRJ34344.1"
                     /translation="MAATQAHALHDDAHAHHHHKEHFITKYIFSQDHKMISKQFLLTA
                     VFMGVMAMIMSILFRLQIGWPGQSFDSLTFFLGEQWAPGGILDRNMYLGLVTIHGTIM
                     VFFLLTGGLSGTFSNLLIPLQIGARDMASGFLNMLSYWMFLISSVIMVYSFYVETGPA
                     SGGWTIYPPLSAHEDFMPGSGLGMTLWLVSMAIFIASSLLGSLNYIVTVFNLRTKGMK
                     MTRMPLTIWAFLITAILGVLSFPVLLSAALLLIMDRTFGTSFYLSDIITANEGMLDAT
                     GGSPILFQHLFWFLGHPEVYIVLLPALGLTSEVIATNARKPIFGYRAMIGSIMAIGFL
                     SFIVWGHHMYVTGMNPFLGGVFVFTTLLIAIPSAVKVFNYITTLWKGNIVYTPAMLFS
                     IGLVSTFITGGLTGIILADSALDIHVHDTYFVVAHFHIVMGMSAVFGMFAGVYHWFPK
                     MYGKMMNTKMGYAHFWLTIICAYGVFFPMHFVGLAGAPRRYYDYTSYPMFDGILDLNV
                     LISVFAIIGAFAQGIFLFNFFYSIFRGQKASQNPWKSNTLEWTTPVEHVHGNWPGAIP
                     EVHRWPYDYSKPGKSEDYCPQVVPLEDGEEDGH"
     gene            complement(19512..20777)
                     /locus_tag="PK610_00070"
     CDS             complement(19512..20777)
                     /locus_tag="PK610_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRJ34345.1"
                     /translation="MTKLIIVLIILLGIIAIAQLMRVLELSAQLRNKREEDISAGDTK
                     LNANLMILFIVSLYVSFIYLLAKYGNGGLPVAASAQGAETDWLLNINFYLIIVVFFIT
                     NTLLFVFASKYAYNKERKAFFYPHNNKLELLWTVVPAAVLAVIIILGLKTWNNITTFD
                     DSEGERVKIELYSKQFEWIARYSGEDNLLGRSDFRLVTGENPLGVQTTASIDATIAGM
                     EKMIVDLDSLYAKSGRPSNPSEVMTEAARAKTKAKKERMQRHLKKVIALRSTMSPEID
                     QQAADDRVIKLKEIHLVVNKDYEFLFRSQDVIHSAYMPHFRSQMNTVPGMVTRLKLRP
                     TITTDSMRTITGDDKFDYILLCNKICGAAHNNMWMKIVVEKEEEYKKWYNEQLPFAGV
                     AAKPEDTIVPTNDSTSTDTTATPSVVAQN"
     gene            complement(20808..21989)
                     /locus_tag="PK610_00075"
     CDS             complement(20808..21989)
                     /locus_tag="PK610_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:cytochrome C oxidoreductase"
                     /protein_id="HRJ34346.1"
                     /translation="MEFTISKRAKNFSYALIGIGLAGTLIGFFMDHSGNSQQKLWSNL
                     LVNGFFFFGVSLIGLFMVALQYATESGWAVVTKRVYEAVMAYIPIGALVLLIVFAASS
                     LHLNHLYHWMDPEAVAHDEIIANKTAYLNIPFFWVRTLVYLATFILFARAFRKRSLLE
                     DQVGGTDIHFKNYRQGALFLVFFAVFSSTMSWDWLMSIDTHWFSTLYGWYVFSGMWIS
                     GIIMVLMLVFYLQGQGHLKAVNESHIHDLGKWVFAISFLWSYLWFFQFMLIWYSNIPE
                     EVTYFQFRIEHYKTEFFLMFAINFILPMLLLMSRESKRHRKLLTIIAMIIFFGHWYDT
                     YLLVMPGTMFDHQHYGLLELGMFLAFLGVFIMVVLRALTKAPLMPVNHPYLEESIHHH
                     I"
     gene            complement(22016..22663)
                     /locus_tag="PK610_00080"
     CDS             complement(22016..22663)
                     /locus_tag="PK610_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRJ34347.1"
                     /translation="MNRSTTIVKFGLSLMVLGLVACSKDPNSPGYEYMPDMYRSPSVE
                     AYVDYSHPDVQSARIPAKGTIPFSEDASKAALNFPYPFHPRSVEEEADMYEKSAVLSN
                     PIAINSENKDVILKKGERIYTQFCTHCHGEKGDGKGSIVNNGKISGVPDYKSANLTAL
                     PDGKMFHSITYGKGIMGSHAGQINKEERWTVIHYIRSLQNDGNYPAIVADSTTIK"
     gene            complement(22669..23202)
                     /locus_tag="PK610_00085"
     CDS             complement(22669..23202)
                     /locus_tag="PK610_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HRJ34348.1"
                     /translation="MANKVIYAMYDDDMVLLEGAKKLVAKGVHVNDVYSPFPIHGIDP
                     VIGVQHTRLGIAAFLYGLTGTLLAIVGIRYFMIVDWPMNIGGKPSFSLIENLPAFVPV
                     TFEFTVFCAAHGMAITYLLRNKTLPGMPARNPDPRTTDDKFVMELHTTENGKFSAEEL
                     QSMLKETGVIELEVREA"
     gene            complement(23221..24600)
                     /gene="nrfD"
                     /locus_tag="PK610_00090"
     CDS             complement(23221..24600)
                     /gene="nrfD"
                     /locus_tag="PK610_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRJ34349.1"
                     /translation="MQKEAAIRTPLILGDKTYHDITEDICRPIESKAPKAWYVLLAIA
                     GVVALWGVGCILYLLGTGIGTWGLNKTVGWAWDITNFVWWVGIGHAGTLISAVLLLFR
                     QRWRMAINRSAEAMTIFAVIMAATFPGIHMGRIWVAYWVFPLPNQFGSLWVNFNSPLL
                     WDVFAISTYFTVSLVFWYIGLIPDFATIRDRMTKPLAKKVYGILAFGWTGNAKAWNRF
                     EIVSLVLAGLATPLVFSVHSIVSTDFATSVIPGWHTTIFPPYFVSGAVFSGFAMVLTL
                     LLIMRKVMHLENYITNKHIEYMNIVIIVTGSIVGVAYLTELFVSWYSGVEYESYAFIN
                     RFSGPYAWAYWSMMTCNVISPQLFWFKKLRTNLTFTFVMSIVVNIGMWFERFVIIVTS
                     LHRDYIPSSWSMFYPTFVDIGIFIGTIGIFFVLFLLFARFFPVLALNEVKSILRVSGE
                     KYRNTENHH"
     gene            complement(24619..27873)
                     /locus_tag="PK610_00095"
     CDS             complement(24619..27873)
                     /locus_tag="PK610_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HRJ34350.1"
                     /translation="MNKQYWKGIEELENAPEFIRNRDNEFPEELPMDAFLGDDKLKES
                     STSRRDFLKFLGFSVTAATLAACEAPVIKSIPYVTKPEDITPGEATWYASTYYDGNDF
                     ANILVKTREGRPIFIKPNRKHGITKGAANARVTASVLSLYDSARLTGPMAAGAPADWV
                     QVDGEISKKLTDIASKGGQIRILTGTVISPSTSTVVADVIAKLGGTAQGTEAAANIVH
                     VQYDPISYSAIRDAHQASFGQNVIPDLDFTKAKTIVSISADFLANWLLHNEYVTQYAQ
                     NRRPENDWMSRHFQFESIMSVTGSNADVRVPIKPSQEGLVAAALYKHVTGSSIGNVPA
                     EILELTAAAGAELKKSAGASLVVSGSNDVNVQTIVAAINHKLGNYETTININNPVNLF
                     AANDRQVEELVKDVTAGKIKAILIDGNINPVYSLRDGNAFAEAIAKMDLSVSFSLVAD
                     ETASKCAYVCPDHHFLESWNDLNPKANHYAIAQPAIRPLYETRNMQESMLVFAGLATR
                     GDKDNDTYYNVIRKNWEAYGFPTQTEIATFEEYWNWNVHNGSGNMAAKPASAVEFAAD
                     VNKAAADLSKISGGEFEVVFYQNTGMGNGSHATNPWLQELPDPITKVTWDNYITMAPS
                     DMEGKYNTKLGQEWPADVATVTIEGLGEVTLPVCPSPGQAKGTIGIALGYGRGANGEN
                     IGKAAYQYEQYGEPTMSDGKHVSVGKNVYPFVVVSGGNLSYRAYKASVSGTGNTYALA
                     STQTHQTVMGRTSVVKETTWGVFKAGDRNAYNKPLTIAYHEGGKQVDKPIGELDLWNE
                     HPVEHIGHRWGMSIDLNTCIGCGNCVVACNAENNVPVVGKDEVRRGREMHWMRIDRYY
                     SSDMTKQRAKEEGVGAISMYSQMENPADSPEVVYMPMMCHHCNHAPCETVCPVAATTH
                     SNEGLNQMAYNRCIGTRYCANNCPYKVRRFNWFNYPSYKKFTSFNPAQSDMGRLVLNP
                     DVVVRTRGVMEKCSMCVQRIQAGKLTAKKESRKLVDGDASTACAEACPTHAITFGDWN
                     DTDSMIRKESSSERAYQALEDVGVKPNIWYQVKVRNIEGAPVAAKSENHEEHSH"
     gene            complement(27900..29216)
                     /locus_tag="PK610_00100"
     CDS             complement(27900..29216)
                     /locus_tag="PK610_00100"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRJ34351.1"
                     /translation="MNNPFRFTFRSFAVITACLYFLLGFSTVNSVQAADGQALFQACG
                     ACHHPEKRMVGPALKGARERWKKNSSEENFYKWIKNSNEVLSSGDAYANQLFKDFNKS
                     PMPAQAVTNEDIDAIFEYVESYTEPVAAVSVNATETAGAQSKEGGWMWIVILGVIFTV
                     IAISAGSVRRQLVNANLEKEGKDELPDLTYWQTWCAWAWRHKVLVSMFALFFIFGGLS
                     DGWYRLKDVGVFENYKPEQPIAFSHKIHAGDNEIACQYCHSTTEKSKHAMIPSPMVCM
                     NCHKAIQKGAITGEEEIAKIYKAVGFDPATMSYTGKTEPIRWVKVHNLPDHVYFSHQQ
                     HVVAGQLDCKQCHGEMKNETVARVMPHEELNAIEGNVKVDRATLTMGWCIECHNKAEV
                     KMDGNGYYTELKQRLMNNKALYQKHLADEKITVEELGGWECAKCHY"
     gene            29474..29995
                     /locus_tag="PK610_00105"
     CDS             29474..29995
                     /locus_tag="PK610_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRJ34352.1"
                     /translation="MRMFLILFLSLASLSLIHSQEVESDWKLFRPVGAIKDTVKRQSN
                     GTSIQQRGSFFQFSQQGKQGDVKVSKDSRIDALSTFVGTPQNGNSTVKIKGYRIQASL
                     DRDKNRINQLRAEYLSRFSEQPAYIDFLAPNFRLRIGDFRSKLDAHAYLDKIKAIFPD
                     AVIVMDDIDLPRL"
     gene            complement(30002..30730)
                     /locus_tag="PK610_00110"
     CDS             complement(30002..30730)
                     /locus_tag="PK610_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34353.1"
                     /translation="MRVVLFLCSLLLFSLYGFGQEPLSNLDQKKTTNNRIYGGLRIGN
                     KALLPWIGYQHHLKKNSFLVFGAGFGNAGSVNEEVFYRRNPDGSYSPTQELEWMTDPD
                     DSIAAPFGTPDNVQLALKFKAITASLQYQVLLIGYVKKKHRLMCYFGAGPSFSFVNQS
                     GFADYFYSGIAQRIEYPVKFASLSIDMEPVLLYEYKQIGIRAGYRLKFGFPFYEGKVV
                     YGTSSGNLFSGFGTDLFLGIGYRF"
     gene            complement(30730..32517)
                     /gene="lepA"
                     /locus_tag="PK610_00115"
     CDS             complement(30730..32517)
                     /gene="lepA"
                     /locus_tag="PK610_00115"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRJ34354.1"
                     /translation="MKKIRNFCIIAHIDHGKSTLADRLLQVTGTLSDRELTEQTLDDM
                     DLERERGITIKSHAIQMEYNYKGEKFILNLIDTPGHVDFSYEVSRAIAACEGALLLVD
                     AAQGVQAQTISNMYLALEHDLEIIPVLNKIDLPGAMPEEVKDQIVDLLGCRREDILSA
                     SGKTGIGVDAVLEAVVERIPAPVGDPNAPLQALIFDSEFNIFRGIIALVKIVNGTLRK
                     GDKVKFFNTGKEYDADEIGTLKLDMVAKKDINCGDVGYVISGIKNAKDVKVGDTITHR
                     ERPCSEAIQGFEDVKPMVFAGIYPVDTDEFDELRDAMEKLQLNDASLVFEPESSAALG
                     FGFRCGFLGLLHMEIIQERLEREFNMSVITTVPNVSYKAYDLKGELTEVHNPSQLPEP
                     SKVSKVEEPYIKAQIITKSEYLGNIMTLCIGKRGILINQNFLTTDRVELSFELPLGEV
                     VFDFYDKLKSLSRGYASFDYYPIGYKESDLVKMDILLNNEQVDALSALIHRSHAFELG
                     KKMCEKLKELIPRQQFEIPIQAAIGAKIIARETIKALRKDVTAKCYGGDISRKRKLLE
                     KQKEGKKRMRQVGRVEVPQQAFMAVLKLD"
     gene            32640..33593
                     /gene="metF"
                     /locus_tag="PK610_00120"
     CDS             32640..33593
                     /gene="metF"
                     /locus_tag="PK610_00120"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRJ34355.1"
                     /translation="MKVIDHLKDAKRTLFSIEILPPLKGKSIQSIFDGIDPLLEFQPS
                     FIDVTYHREEYVYKKREGGYLEKVSIRKRPGTVGICAAIMNRYKIDAVPHIICGGFSK
                     EETENALIDLQFLGIDNVLALRGDSIKTEPGFVPDPDGHHYALDLVQHIDKMNNGIYL
                     EDDVKDMAPTNFCIGIAGYPEKHMEAPNMASDIKFLKMKVDAGAEYIVTQMFFDNKKY
                     FEFVDLCRKNGINVPIIPGLKILTGKKQLTGLPKIFHIDLPEELYENVEKAKDDKEAK
                     EVGIDWCIRQSKELIAKGVPCLHFYTMGLSDSTRRVAKEVF"
     gene            complement(33594..>34312)
                     /locus_tag="PK610_00125"
     CDS             complement(33594..>34312)
                     /locus_tag="PK610_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HRJ34356.1"
                     /translation="EPYIHPQFLDLVHYAKSKGIYTSTSTNAHFLSDEKAKSTVESGL
                     DRLIISIDGTSQDTYEQYRVNGSLDKVIEGTKNIIRWKKELRSSTPHIIFQFLVVRPN
                     EHQVDEVFQLAKELGVNEVRLKTAQVYNFENGNPLIPENEKYSRYKKKKDGSWSVKNK
                     MEDHCWRMWSSSVITWDGKLVPCCFDKDAEYRLGDLKQESFRSNWTSPAYNQFRNAVL
                     SGRDQIDICKNCSEGTKVWA"
BASE COUNT        10353 a         7465 c         7164 g         9330 t
ORIGIN      
        1 ccccttcaga atagttgtta tgatcccaaa tgatatgtcc ctccccatct acaagaaagg
       61 tatggggaac gttgtttacg ttcattgcgc gtttgaaatc ctggttttca tcaatgtaaa
      121 catcgtaagg taatcctttt gaaacgatat acggttttac ctttgatgcg tttcgcgcgt
      181 cgtcaatcga aatagcaatg atttttactc cggtttcttt ctgccaatct tcgtaaacct
      241 cagccatgtt ttccaattcc tttttacaag gagaacacca ggtagcccaa aaactgatta
      301 cgatgggttt tccaccattg gcaacatcag ccatgttaaa tgtgccgcca tcaagcgtct
      361 tcacttttac agccggtact tttcttttat ttccatcaac cgaggaagga tcaatttttg
      421 tcgcgctgaa agcaaacatc aaaattgcag aagaaaaaag gataattctt ttcatgattc
      481 ttgtttttca tagtggaatc aaaattcaga ccacaacaca ataaaagtaa ttacaatcca
      541 tgtcttaata aagcaattgc gtaaataatt gtgggattgc taagacttca tttaaacggc
      601 tctcaaggga tcccctcact ccactactaa acaaacagac tgataaacaa catccttcgt
      661 gcttatcttc aaataataag aaccctttgc cagcccggta agattaaaat cctgactctg
      721 tattccaggt agaataaccc ctctaaccac ttgcctgcca tcaacaccga taattgaata
      781 ttctgcttct gcatctaata gactagggaa actaacttca aaactccctg ctgaagggtt
      841 aggccatgtt ttcagcatta ctaaatagct ttcatctgtt cctaaagctt gcatgatacg
      901 gatatcaaag ataatcctga tggtgtcggc cggatagtct gtagtaaaca aatcaatatg
      961 gacttctcca aaaccgggat aggcattatg aaaaatattg atgatgatct tatccatagt
     1021 tcccgcaatt ggaggaagga taaaatcaga gctgtcgggc gcaggattat gatacgttat
     1081 gttgtcctga attgctattg accattgcga tggataataa gtatccgccg gatcaatcct
     1141 ccaacgcata ttcagagaat cgcctgtatg gttgaataat tgaaaatgct catgaggagg
     1201 agtaattgat gctgtggttt ttactatagg tttataggtt gtatcaactg aaaatacctg
     1261 agtaattccg aataagggaa taaacaaaca accaaaaatt gtcagggaaa tacgcatgag
     1321 ataaagttac aaaaaaatag tccctgacat ttctgccagg gactattata ttcaactcat
     1381 agtgtaatta gtgtgtaaca accactttgc gtgtgatcat gtttccacca acgtttacgt
     1441 tgacgaaata gatccctgac tcaagacggt tcgcatcgat tacaaaattg ttttctccaa
     1501 ccaatgttcc atagttaaag cttgaaacaa ttgcacctgt gatgctgatg atggaaatac
     1561 tcacctctcc ggcttcctga atgttcaact taacagtagt catgtcgttt gcagggttcg
     1621 ggaagattga aaagtcgaaa ttatcaactc cgtcttcttc aattccaaca gtcatgtgtg
     1681 ctttagcagc gttaaggatt ttacctgttg cattatcaat caacacgcca ataatctcaa
     1741 tatcattttc atcctgacct gcaggaagag tatggttaaa ggtatagttt gtgttgcttc
     1801 ctgctgttac agacgttggc actgaaccag tttgacctgc aaatccacct aacaaagcac
     1861 gaccaacatg atcgtattcc atttgagcag caggaatagg attaggggaa gtctgccagt
     1921 tgtgtccagc cccggtaaga gcaatgtttt gggaggttga gctgtaatag tttgtctgat
     1981 tgtaaccgga tgttgttcct gttacattgt tctcaatcaa aattgcacct aaacggaagt
     2041 taaggttatt gaatgaacct gcccagtttg catttacagt taccgtcatt gcacgtgatc
     2101 cggcattcca ggtattggat accgaaatat tagctggtgg aactttagtg atcatttgat
     2161 tgtaagtagc agggaattgg ctaggatcaa tatcaagtgc atcacgatca acaacaccgg
     2221 aaggatatcc accaattaaa ccaccaattg cggcatcgta agcagttact gtcattggat
     2281 cgctgttatg tactgcaact ccaacccaag tattaggata gttttgctcc atttgcgcca
     2341 tgaaaacgtg accgcgtgga caccatccgc accatgtacc ggttccttct tcaccaagaa
     2401 cacgcttggt aggtaagaaa tttaatccgt agattgttgc atatgctgta tcatttgctg
     2461 tatttggatc tccagcaagt tcagtccaaa ctttgatgtt ataatttgtt cctgctgcaa
     2521 cagccaggct cgcagaatgt gagtaattat aggacgctcc tgatgcgata tttaaaccag
     2581 ttaagttctg gttatgcgta gttgttccat cagaccactt aagagttaca gcagtaattg
     2641 gggtagcgcc ggcattataa atagcaccgg tgatgttaac gtttgcggga cctacaacga
     2701 aaggattagt tgtaattgaa gtaactgatg catcattagg attcaattgc agaacctgaa
     2761 tatcatcaag catcaataca aacatatcgt ttgagtagtt ctggaatccc aaacgaatgg
     2821 tttgaccggc atatgcatca aggttaatct gacgccatgt ccatgtagtt gcatcagcag
     2881 gaatatttaa aagctgtgca ccacttccac ctgtgaaatc agcaggagct ccacctgagg
     2941 cggtagtaac atatacacgg taaccatcag gataacttgg atcctgagcc atagatctcc
     3001 agcggagtgt taggccggta cctgaaggga gtgtgatttg tggtgtcgcc atccagtcgt
     3061 tggaaacgcc ggctggagta taccatgaaa tagaagcagc aactgtattg ctggcactgg
     3121 cgaaatctgc acgggtaacc caagcgttgg tacaccagtt aacattcgtg gcgggggtaa
     3181 gaccatcaac attggtcatg atccatcctg caggcattgc aggagtagtt acggattgga
     3241 aattttccga gaaaatgaca gtttgtgcca ttactgggaa tgctccaatt accgctgcta
     3301 cggaaagtaa gagtttcttc ataaggtttg tttttttgat ggttgtattc attatttgca
     3361 ttccaaaaat ctatcatcct gaactggaaa acaagttagt cctggtgttt tttagattta
     3421 ttctttgaat cctaagttaa tgatttgtca accaaagaca ccgtatgccg catttttgca
     3481 accgaaacct cagagatata aaacctttac ccctgattat ccgtagaata gtgcggattc
     3541 aaaccaatcg ggatttgtca tcattcttgt tctaattcca tttaggctat ttttaaaaca
     3601 tagtgggatt tgagccggaa gtccttaaat ttgaacatca ttaatatctc atcctatgaa
     3661 aagacaatta ctcgctcttt tatttgcttt cggcttttta gctgtgaatg cacagtcatt
     3721 tcagtttcac gaccaaagcg gaaataattt atccggtcaa acagtaacac ttaccggtga
     3781 tgattctgaa tatgagctta ccgactacat caagctaatg aacaatagcg gaaccagtaa
     3841 aaacataaaa attaagcgtt acgaggtttc tgtcactcca gctaccacta attatttttg
     3901 ctggactgtt tgttacgctc caatgacagc cggtgcgtat ccacagtttc cctacgtagg
     3961 acatcttgca tggaatgatt acgtaactgc ttcagggagt gcttatgctc cttatcagtt
     4021 gattgtttat cacaagccca atggaaacgc tggaaattcg ctctaccgct atgtagcttt
     4081 tgatggtgca aacccgaacg actctgttga ggtttttgtt gccgtaaata tttctgctgc
     4141 aaatagcgta aatgaacttt tcgctgtaaa atcactgaat ttgtttccaa atcctgctaa
     4201 ccaggttgcc aatatccagt accagtttaa taatgctgca ggcgaacaac aattggtagt
     4261 tagcgatatg attggaaacc gtcgtaaagt gatgcagctt attggtacag aaggtaccgt
     4321 tcgtctggat ctcaatgaat atgcttcggg tatttacttt gtaaccgtaa tgcgaaatgg
     4381 tgaagcggta tcaaccaaaa gattagtagt aacgcactag cctaccggtt ctaataataa
     4441 ttgctgagcc agtttgggga ttcccgaact ggctttttct tttatgatct ccaaaccatc
     4501 aatatcatta agatgaatgg tatgcggatc taccagaaat tttttacacc tgaatccggc
     4561 ataacgaaca aggtttgcag caggataaac tgtaagtgat gtaccggtaa taattagcac
     4621 atcggcacct tcaacaattt tttgagcaac aggtaacaaa ggaacttctt cgccaaacca
     4681 aactacgtgc ggacgcaatt ggaatcctgc aggacattta tctccaaaat tcagattatc
     4741 gcctgatatg gggtacaaat gctggtcctt tgggtttgta cttcttgcat aacggatatt
     4801 accgtgtaaa tgaaccacat gtcttgaccc tgcacgttca tgaaggtcgt ctatattttg
     4861 tgtaattacg gtaacatcat aagccttttc caattcaacc aaagagatat gggcaagatt
     4921 aggttttgcc tccagaattt gagccctcct tttgttgtaa aactcaagca caagctcctg
     4981 atccttaacc catgcttcag gtgtagcaac gtccatcacg ttgtactcct cccacaaacc
     5041 atcagaatcc ctgaacgttc gtaaaccgct ttcagcgctg atcccggcgc ctgaaaaaac
     5101 aacaagtttt ttcttcttca tagaaatgag gctcaattta cattttttcc cacaggtccg
     5161 caaccaaatg agggtctcaa ttcagtcctt tttttacttt tacctttctt attctgaccc
     5221 tacatgactc aaataaaatt caaaaagcaa gacgcgcttg attatcactc atccggaagg
     5281 aagggaaaaa ttgaagtcat acccacaaag ccttatgcct ctcagcggga tctttccctg
     5341 gcctattctc cgggcgttgc cgagccttgc ctggctatca acaaacgcaa ggacgatgtc
     5401 tacaaataca ccgcaaaagg aaatttagtg gccgttatca gtaatggcac tgccgtactt
     5461 ggactaggtg atattggtcc tgaagcatcg aagcctgtaa tggaaggtaa aggcatgctc
     5521 tttaaaatct atgcagacat tgatgttttt gacattgaaa ttgatgagac agatgttgat
     5581 gcttttgtaa aagtagttaa ggcaattgct ccaacttttg ggggtattaa ccttgaagac
     5641 attaaagcac ctgaatgttt tgaaatagaa cgacgcttaa aggaagaact cgacattcca
     5701 atcatgcacg acgatcagca cggtacagct attatttccg gagcagcgct attaaatgca
     5761 ttggagttgg ccgggaaaaa gatcagtaaa gtaaaaattg ttgtcaacgg agcaggagct
     5821 gcagcgattt cctgtgcaaa gctctatgtt tcattaggag caaaaaaaga aaacatttta
     5881 atggtcgatt ctaaagggcc gataaccaaa aacagaaagg accttgacga gcataaatca
     5941 ttttttgcaa gtgcccgaaa agtgaattca cttgaagaag cgatggaaga cgcagatgtt
     6001 tttcttgggc tttccaaggg aaatattctt tcaaaggaaa tggtaaagtc gatggcgaaa
     6061 aatccaattg tttttgccct tgcaaatcca gaccctgaaa tttcctataa agaagctaaa
     6121 actgcccgtg aagatgtaat cgttgcaaca ggacgttctg accaacctaa tcaggtaaac
     6181 aatgtattag ggtttcctta tatattcaga ggcgcgcttg acgtaagagc gaccaagatc
     6241 aacgaggaaa tgaagcttgc tgctgtcagg gcaattgctg agctggcaaa agaacccgtt
     6301 ccggaagaag taaatgaagc ttatggtgag aaaaacatca gctttggcag agaaaacata
     6361 attccaaaac cgctggatcc acgcttattg tggaccgttg ctcctgcagt tgctaaagca
     6421 gctgttgatt cgggtgtcgc taaagaacca atgaaggaca ttgatgccta catcgaagag
     6481 ctccagaaaa agaccgggca tgccaataaa ttgcttcgaa gcatttctga aaaagcaagt
     6541 aaaaacccga agcgtattgt atttgcagaa ggcgataatt ataagattct taaagctgct
     6601 caaatcgctt ccgacgaagg aattgccact ccaattttat tgggagatgc aaagaaaatt
     6661 cgcggactca tcgaagagta ttcgttggat cttgaagatg cagagattat tgacccaaaa
     6721 gatgcttctg agtacgaacg cagagtaaaa tacggagagc ttctattcaa aaaacgtcag
     6781 cgaaaaggaa tgaccttgtt cgaagcgcaa aaggcaatgc gtgaacgcaa tcatttcggc
     6841 gcaatgatgg ttgaagttgg tgaagcagac gccttgatta gcggtataag cagaagttac
     6901 aaggaagtta ttcgcccctc acttcaaatc atcggaacag cagacgatgt agagcgggta
     6961 gcaggtatgt atgtgatttt tacaaaagac ggaccagtat tttttgctga taccactgtt
     7021 aatcacagtc cgaatgccga agagattgtt gacattacat tgatggtagc aaaggctgtt
     7081 cgcaatttcc gcatcacccc aagaattgct atgttgagct attccaattt cggatcagca
     7141 aatggcgacg accctgataa aatgcgcaaa gccgtttcga ttctgcacaa ggaacatccg
     7201 aaccttattg ttgacggaga aatgcaggct aattttgcct taaatcagga tttgctgcag
     7261 gagttttttc cattctccga tctgaatggt tcaaagccca atacattcat tttcccgaat
     7321 ctggcatctg gaaacattgc ctataaaatg attcaggaaa tgaccaatgc agaagttatt
     7381 ggacccatcc tcctgggtat gcgcaagtcg tttcatgtat tacagattgg ttgctcggta
     7441 agagaaattg taaacatggt gaaaattgca gtggtggatg cacaaacaaa aagtaaaaag
     7501 taaatgatcg aattcatcaa aggcaggctc actgaaaaaa accctgctta tgtagttata
     7561 gaagcaaacg gaataggtta ccttctgcat atttctctca acactttcag tcagattgga
     7621 aaagatgaaa atgtatcctt gtacacccat caggttatcc gtgaagatgc acacctcctg
     7681 tttggcttca gcacaaaaga agaacgttcc gtattcagat ccctattaag cgtatccgga
     7741 gttggggcaa gcactgcccg aatggttctt tcatcacttg gtccggatga agtacaacaa
     7801 gccattgcga tgagcaatgt atctgctttc cagaaaatta aaggtatcgg agccaaaaca
     7861 gcccagcgca ttattgttga tctttcaggc aaaatcgaaa agatagaact tgctggcaat
     7921 caacctggag gagtgcttcg acaagaggcc ttgggggcta tggtaaccct tggctttgat
     7981 aaggcacaag ccgaaaaggc agtagataaa gttttggcca ctgataataa tctccccgta
     8041 gagcagttaa ttaagctggc tttaaaaaac ctttaacact ttttaacgga cttgagcgca
     8101 acaacaacca taagaaaaac ggttctaact ataggttctt cacttatagg actatttctt
     8161 attgcgttcg ctaataccgt ttttaaaggg aatatcgtaa gccgatttgt tgggccacct
     8221 gtggctgact ctccaaccgt taatcttccc taccctattt acccacccaa tagcagcaat
     8281 tccggcaact caagtaatgt taaccttcaa aatccgctta atttttcgga cagtgtagtt
     8341 tacgatccaa ctaacggaac atacaacttt aactatcagg taggaaattc cataccctat
     8401 cagccatcaa gcagtatgac actggaagaa taccttgact acgatatgga aaaagccctg
     8461 caaaaaggat ggcaggatct ttcagaagaa tatggcaaaa aacctggtgg tgccgacata
     8521 ccctttctca aaccaaaagc tgaaggagga atgttcggta gcgattttat tgacattaaa
     8581 ccatccggaa ctgcagagct gcgatttgga atcaacacat ccaaaaccga aaatccgcaa
     8641 ataccggaaa agcaacggaa gatcactgta ttcgattttg atcagcgtat tcagttaaat
     8701 gttgttggaa caattggtaa taaactaaaa ctaaacacct cttataacac acaggcaact
     8761 tttgattttg aaaatcaaat gaagcttgaa tacaaaggcg atgaagatga gatcattcaa
     8821 agtattgaag caggtaacgt tcaacttcca ttaaccggtt cccttatcac aggaagccga
     8881 tcgctttttg gaattaaaac acaattaaaa tttggaaaac ttacggcaac aactgttttc
     8941 tcgcagcaga aaggtaaacg tactgaagta gaagtttcag gaggcgcaca agtcagtaat
     9001 ttcgaattaa cagcagataa ttacgaagcc aataagcact atttcacctc tcacttcttc
     9061 agaaatactt ttgataatgc gatggcagcc ttacctcagg ttgcctctca ggttaacatt
     9121 acccgtatgg aagtttgggt aaccaacacc aacaacactg ttgataatac ccgaaacatt
     9181 atcgcttttg ccgaccttgg agaaacgtcc atttttgaag gtgctcccaa tgcaatcaac
     9241 ggcaccttgc ccagaaatga agccaacggc atttatgcat ttgcttctgc taaccccaat
     9301 attcgcggat ttaacaatgc taccgcagca ttaaatgccg tagtaacatc acccggacct
     9361 tttcaacaag ctgtgcatta cgaaaaagta cagaacgcac gtaaacttac cgatcaggaa
     9421 ttccaattca atcccatgtt gggatttgta acactcaatc agccgctcaa caatgatgaa
     9481 gtgctggctg ttgcatttca atatacctat cagggacaaa cataccaggt aggcgaattt
     9541 tcaaccgatg gggttgcagg tcaggatgca cttgtattga aacttttgaa aggaaccgtt
     9601 accaatccga tctacaaaag gtgggatttg atgatgaaaa acatttattc tctcggtgca
     9661 taccaggtga accgcgataa ttttaggtta gacatctggt acaacaatcc tgcaaccagc
     9721 gttgacatca atttcattcc acagccagga gtagataata tccccatcat tcaattactc
     9781 aacctcgata aattcgatcc caataacaat cccttccccg atggtgtttt cgattttatg
     9841 ccgctaactt tctcgggaaa caaagccaat ggagggggga caataaatgc aacaaatggt
     9901 agagttatat ttaccactgt tgagcctttt ggcggaacac ttagaaataa acttctcgtt
     9961 gccggtgttg accccactgt tgtaaatacg attgtctacc aaccacttta tgattctaca
    10021 aaaactgcgg cacagcagat tccacagttg aatcgtttca aaataaaagg aaggtaccaa
    10081 tcatcaacca gtagtgaaat ttcacttaac gcattaaata ttccgcaagg tgcggttacc
    10141 gttactgcgg gtggtagaac tttagttgag aacagtgatt atacggttga ctataatctt
    10201 ggtcgtgtaa gaattattaa taccggatta cttgaatcgc aaacgccaat tcgcatttca
    10261 gtagaaagca acacattgtt tagcatgcta acgaagacaa tgctcggaac acggtttgat
    10321 tacaggttaa gcaaggacgc aagtctcgga gcaaccgttc taaatatgac agagcgtccg
    10381 cttactcaaa aaataaatat tggagatgag ccagtgagca atacaatgct tggtttcgac
    10441 ggaagtttca aaaaggaagt cccctacctc acccgacttg tagataaact cccgctgatt
    10501 tctacaaagg aaaaatcgat gttgtctgca tctgcagaat atgcaaccat catccccggt
    10561 acagccagag cgatcaacgg catctcctat atcgacgatt ttgaaggaag tcagtcagca
    10621 attgatatcc gctctttttc ccaatggcaa cttgccagtg taccaaaggg ccaacccgat
    10681 ctgtttccgg aaggtagttt gatcaacaat cttgactatg gaaaaaatcg cgcactgttt
    10741 aactggcacg ttgtagatcc gcttttctac aacaataaca atctgacacc acaacacatt
    10801 aaaaattcgc caatgcaatc gaaccacacg atgcggcaaa tttttgaaca ggaggtattc
    10861 ccaaacaaac aattggcaca aggacagcca acaaatatcg caatgttcga tattgcattc
    10921 tggcctagtg aaagaggtcc ttataattac gacacatctc catcagccta ttctgcaggt
    10981 ttaagcggca ccggattact taacgctcca gattcaagat ggggtggaat tatgcgtcag
    11041 ctacaaacca ccgacttcga gttagcgaac attgaattca ttcagttttg gctcatggat
    11101 ccgttcaata tcgatgcaac aactgatctt ggtgtaacag gtggtgattt gtatttcaat
    11161 ctcgggaatg tatcagaaga catcctttca gattcacgca aaagctttga aaatggtttg
    11221 ccaatttcaa ccaacttcga tccgaacaca ctcgacacaa cggtatgggg actggtggca
    11281 actacacaac ccatcgtaaa cgcttttgag aacagcagcg accccagtat tcgcttacga
    11341 caggatatcg gtctggatgg tttaaactcg caaacagagc aagcattttt ccagtcgttt
    11401 tacaattgga ttggagccaa tgtaactgac tttaatgctc gcactcaaat tcaaaacgat
    11461 ccatcgaatg atgattacaa ctattaccgc gatgatcgtt atgatgcaag tcagctgaat
    11521 attttgcagc gttataagcg ctataacggt caagaaggaa actcaccaac caacgagttc
    11581 tcacaaactc taaactccga cggctatcct accagtgcct ctactattcc caacgtagag
    11641 gacatcaatc aggacaacaa tttaagtgag gcagagtctt acttccaata caaaattagt
    11701 ttgcgtcctc aagacatggt gattggtcag aatttcatta cggacaaact caccaaaaca
    11761 gttacacttc ctgatggaag caccaaacaa attacctggt atcaattcaa ggttcccatt
    11821 cgcaaatatg aaaaagctgt taacggaatt tcagactttc gctcgattcg atttatccgg
    11881 atgtttgtaa aaaacgctca aaatcctctc attttacgat ttgcacgctt ggaattaatt
    11941 cgtggtgaat ggagaactta cattccttct cttcttgatc cgggagaata tattcaggga
    12001 gaccccaaca gcacaacctt taacctgttt gcagtaaata tcgaagagaa cggtaaccgt
    12061 gtcccaacaa attatgtact tcctcccggg attaatcgtc aggtagatgt ggccacagca
    12121 aatcttcgta acctcaatga gcaatcgctg gcgtttgaag tttgtggatt ggaagatggc
    12181 gacggaaggg cgtgttacag aaacgtagca ttcgatgttc gctcctataa gcgtttaaaa
    12241 atgttcgttc atgctgagac aacaagttta actgagcaag tcaccaacaa caaggatgtt
    12301 acccttttcg tgcgattggg aactgacttt acggagaact tttatgaagt agaacttccg
    12361 ctgatcatga ctccatgggg tgcaacagta gataccgaca tctggccatc ggcaaacgaa
    12421 ttggatattg attttgaagt cctgcgtaat cttaaattgg aaagaaacag tcgtgtgctt
    12481 gcaggattgg cggcaaacaa tcaacttttt attcgtaatg atcccggtta cccggatcga
    12541 ttgattaaga tcatcggtaa ccccaacctt cagggattaa aagtggtaat gataggagtt
    12601 cgcaatccaa ataaaaacac caatccatgg gatgcttctg ataacggcac tgctaagtgt
    12661 tttgaagttt ggatcaacga attgcgatta actgattttg accaacaagg tggatgggct
    12721 gcgatgggaa gggtaaatgc taattttgct gacttcgcac aagtagcatt atccggaaat
    12781 tacagtactc cgtattgggg aagcatcgaa aagaaagtta gtgagcgtca acgcgaaaca
    12841 agatacggtt gggatggatc atcctctttt gagttaggta aattcttccc ggaaaaatgg
    12901 ggaatccgaa ttccgatgtt cctcgggtat tcacaaacga tcatcaagcc gcaattcgat
    12961 ccgcttaatc ctgacattcc ctttaatgat cttccatcgg accagcaagc catttccaaa
    13021 caaaaagtaa ttgattatac ccagcgaaaa agcatcaact tctccaacgt aagaaaggaa
    13081 cgaacaggag ggaaaaaagc acacttttat gatattgaga attggtcagc aacctattcc
    13141 ttcagcgaaa cctataaacg tgatattaat accgaataca atatcagtaa atcaatcaga
    13201 ggcgggctga attataattt caatgccacg ccaaaatcgt gggaaccatt taagtcgaac
    13261 aaactaattg gtaaatcaaa atggtttacc atcatcaaag acattaacat cagtaaggga
    13321 ccaaagctgg tagccttccg taatgattta aacagaacat acaacgcata tcagatcaga
    13381 aataattttg acggaattgt attccccact tataataaaa atttcatttg ggttcgcgca
    13441 tacgatttca aatacgacct aagtaaaaac atcaagttcg attttggtgc caataacaat
    13501 gcagttgtag gagaaccact gggacgagtt gatcgccaat accgcgatga ataccaggta
    13561 ttcaaagaca gtgtgatgca aagtttgcgt tcgtttggaa ttaataaaat gtacaatcat
    13621 acaggaaatg taaatattac ctggcccatc aataagattc ctgctcttga ttggatcaca
    13681 ctcactacgc gttattcagc ctcctatgaa tggcaacgcg caccattcgg acaagatact
    13741 ttaggaaata ctgttcagaa ctcgcgcaac gttaattgga atgctcaatt gaacttcaac
    13801 acgctgtaca acaaggtgcc ttatttcaaa aaagtaaatc aaaaactaca gggcagcggc
    13861 ggtgcgaagc caggtggaaa accatctacc aatagcaaag agacacctaa agaaggtaaa
    13921 gacggtaaaa caagttcgga ctcaacaaaa aaagagaaga aagaacctct atttacagtt
    13981 ggtgaaaata ttgcccgggt tgtaatgagt gtaaaaacac tctcgctcac ctactcaaca
    14041 actgatggta ttatgatgcc aggatttggt caaactaatt ccgttcttgg catggataac
    14101 caatggaatt cgcccggatt ggggtttgtt gccggaaaac aaaacaaaga tctgtttgga
    14161 cgcgaatccg actggggcaa tggatataac gattatgcta tgtatgcagc agatcagggt
    14221 tggctggtta aaaatcaatt cctgaatgct caatacactg caaatcatac aaaaaatctt
    14281 tccgccagaa catcacttga accgttaacc gggttgcgaa tagaattgaa tgcagataaa
    14341 aaccaaaccg aaaattataa cacattcttc cgttggttcg atactttaaa tggcgcacct
    14401 ttcttcgatt acacagcgca gaacccaatg acacagggaa tgctgagcat gtcgatgaac
    14461 acatggaaaa ctgcatttat tggcaatgga gaaaacaaca gttctccggt tttttccaac
    14521 ttcagggata tccgctcaga agcatcacaa ctctttgcat ctggaaatcc aaactcctcc
    14581 cttctttcga gtagcgagac cggttatgca ggtggctatg gaagcaccca acaggatgtg
    14641 atgatcactt cgtttattgc tgcctattca gggaaaaaag tggatcagat tggctccaac
    14701 ccattcgcac ttcgtccggc acccaactgg cgagtcactt tcgacccaat ggcgaaatca
    14761 aaaattaagt ttttgaaaaa atggtttaaa tcattctcca tttcgcatgg ttacagatct
    14821 acaatgacca tcagtaacta tacaaccaat ttattgggaa cacgagacac gcaagggaat
    14881 atggatgcaa ccgacatctc cggaaatttc ataaacccac gccagatcag catgattacc
    14941 atcagcgagc aattcagtcc gctgatcaat atcgacatga cctggaactt aaaaaaccgc
    15001 ggcaaacccg gtggattaat caccaaagtt gagcttaagc gcgatagaaa tctgtcctta
    15061 agtctcgtaa acaaccaggt aactgaagta cgtggagcag agtttgtaat tggttcaggg
    15121 tatcgcttta acaaccttaa gcttccgttt aacattggcg gaaaaccaac agagagcgat
    15181 ctgaattgca gaatcgacct ttcagtaaga aacaatgtca cgtacacccg caaagtcgtg
    15241 gaaaatcaaa accaggcaac agcaggtcag cgcattgtat ctattaaatc ctccgcagat
    15301 tacgctatca gcagtaagct aaccattcgt ttcttctacg acaaagtatt aaaccgtccg
    15361 gttatctcaa cttcattccc cacttcgaat actaattcgg gtattgcaat tcgctttacg
    15421 ctctagtgaa aaaaattcta cttttggggc aaatagaact atcatgaata ttcctgcaaa
    15481 cctcaagtac accaaagacc acgaatggat tcgcattgaa ggcgattttg caattgttgg
    15541 tattacagat ttcgcacaag gtgaacttgg cgatatcgtt tacgttgaaa ttgaaacttc
    15601 aggcgaaaca ctttcaaaag aagaggtttt cggcacagtt gaggccgtaa aaacagtaag
    15661 cgatctattt atgccggtta gcggtgaaat cactgaggtt aatcctgcat tggaaggcaa
    15721 ccccgaatcg gtaaacaaag acccttacgg agaaggctgg atgattaagg taaaacttag
    15781 taatccatct gaattggatg cattgctaag tgatacgcaa tacaaggagt tggtaggtta
    15841 atacatgcta ttgcgttttt tgtttccggc gatttgctgg gcgctgctga tttttcttct
    15901 ctgtattgca ccaagcgacc agcttccgga cacaagtatc tgggatttta tctcttttga
    15961 taaagtcgcg catctcttca ttttttcagt attcagctta ctgattattg ttggattacg
    16021 aaggcaatat tcctattcgg ctttacgtta tcatgccaag cgagtggcgt ttctagctgc
    16081 aagtgtttat ggggttatta ttgagtttat tcagcaggtt atgaattccg gaagacattt
    16141 tgaatggatg gacgtggtag cagatgttat tggttccctg ttgggaatca cgcttttccg
    16201 gattatttac ggcaaggagc tcgccagatg aaccttttga aatttgcagt gtacagaata
    16261 aaaacgtaaa ttagcaacca atactttcct tatggagatc aagaaaaatc ctgatgtaga
    16321 catcaacaaa aagctccctt cactcaagat gatgggattg ttggtggctc tcgcctttac
    16381 gcttactgcg ttcgaataca cagtattcga aaaagaagaa ttggcgctca attatgacac
    16441 aaccaacgat gacctcctcg atgagcaaat tgttgacatc ccaccgccgc caccgccacc
    16501 ggcaccaccg ccgccgccaa tgattgttac tgaaatcaaa acggttgaag acgataaaaa
    16561 agttgataac gttcaaacat ttcaggaaac agacgtgaat gacaagatcg acatcgttga
    16621 tgttaaggtt gaagaagcac cggtagtaga agaaatcttt gacgtagtag aagaacaggc
    16681 agaattccca ggtggaatgg aaaaattaag agatttcctc tccgctaatc tgaagtttcc
    16741 ccctatggca aaagaatccg gagtacaggg aaaagtctat gtacaattcg ttgtatttaa
    16801 agatggttct gttggagaca ttaaagtact tcgcggaatt ggttcaggtt gcgacgaaga
    16861 agcagtacgc gttgttaaat ccatgccaaa atggaacccc ggtaagcaaa gaggtcgcgc
    16921 ggttagctcg cgatttactt taccaatcaa tttcaagctg caataatctg cattcataaa
    16981 acaaaaaagg ccctgcatac agggcctttt ttatttgccc ttctttgtaa tatcccgcca
    17041 taagtcacca acaaacaact tacttttcag gatcaatgtc ggtgaaaaaa actgttccgg
    17101 aattgcagtg cgattgatta tcccaatgcg atagccaatc ccgcagccaa ccccaaaata
    17161 tcgccaaaat cggtactgaa tggtcatcat aggctcataa accacaaatg ccttattggt
    17221 gcttctaccc ccaacaaact cgtacctggc ccaaccggct ccaatttgtg ccgggattgt
    17281 tgcctcccag tgataattcc gaaagaaact atattcccca tacacgctga aatagtaata
    17341 cttcaactga ctggaccttt ccattccctg aataaaacga tcctgctcag gaaacttctt
    17401 gttaagccag ttaaacccaa tgcccaattt aaaggagcca ccgtaatcaa ttcccacacg
    17461 gatattggac attgttgcaa gacggtttga aatgaaacaa tactgcgatc cccaattaaa
    17521 atataagcct ggtttctgat caaatgcttt tgagatggta tcaaacattt gtgcgtctgc
    17581 cctaattggc aaaaagaaaa gtaaaaccaa agaaatacga ggcagccaca taaaaaaatg
    17641 tccgcaccaa ggtacggaca tttcatatcg ttgtaaagac ggattaatgt ccgtcttctt
    17701 caccgtcttc caagggaaca acctgcggac aataatcttc cgatttaccg ggcttactgt
    17761 aatcgtaagg ccaacggtga acttcaggaa tagcgccagg ccagttaccg tgaacatgtt
    17821 caacaggagt agtccattca agtgtattcg atttccaagg gttttggctc gctttctgac
    17881 cgcggaaaat gctgtagaag aaattgaaaa ggaagatacc ctgagcaaat gctccgatga
    17941 tggcgaaaac agaaatcaat acattcaaat ccagaattcc atcaaacatt ggataagatg
    18001 tgtaatcata ataacgacga ggtgcgcctg cgagtcctac aaagtgcatt gggaaaaaca
    18061 caccgtaagc gcaaatgatg gtcagccaaa aatgcgcata acccatttta gtgttcatca
    18121 ttttaccata cattttcggg aaccaatggt acactcctgc aaacattccg aataccgcag
    18181 acatacccat cacaatgtgg aagtgagcca caacgaaata ggtatcatgc acatggatat
    18241 ccaatgcaga gtcggcaaga attatacctg ttaatccacc ggtaatgaac gttgaaacca
    18301 ggccaatgga gaacaacatg gcaggagtgt aaacgatatt tcctttccac agtgtggtga
    18361 tatagttaaa tacttttaca gcagaaggaa tagcaattag taaggttgta aatacgaaca
    18421 caccaccaag gaatggattc atcccggtaa catacatatg gtgaccccaa acgatgaacg
    18481 aaaggaaacc gattgccata atggaaccaa tcatcgcacg gtatccgaag atcggtttac
    18541 gtgcattggt tgcaattact tcagatgtaa gaccgagtgc aggaagaagt acgatatata
    18601 cttcagggtg ccccagaaac cagaataagt gctggaaaag aatcggactt ccacctgtag
    18661 catccagcat tccttcgttc gctgtaataa tatcagataa atagaagctt gttccgaaag
    18721 tacgatccat gataagcaac aaggctgcag aaagaagaac cgggaatgat aacacaccta
    18781 agattgcagt aataaggaat gcccaaattg taagaggcat ccttgtcatc ttcattcctt
    18841 tagtacgtaa gttgaaaaca gtaacaatgt aattcagtga accaagtaat gaagatgcaa
    18901 tgaagatcgc cattgaaacc agccaaagcg tcataccaag gcctgagccg ggcataaagt
    18961 cttcgtgcgc gctcaaggga ggatagatcg tccaaccacc ggaagcaggt cctgtttcaa
    19021 cataaaacga gtaaaccatg ataacacttg aaataagaaa catccaatat gaaagcatgt
    19081 taaggaatcc tgacgccata tcacgtgcac caatttgcaa gggaatcaga aggttactga
    19141 acgttccgct caaaccaccg gtaagcagga agaacaccat gatggttccg tgaatggtaa
    19201 caagcccgag gtacatgtta cggtcaagaa ttccacctgg agcccattgc tcgcccagga
    19261 agaatgtcaa tgaatcaaaa gactgtccgg gccaaccaat ttgcaaacga aaaaggatag
    19321 acatgatcat tgccataacc cccatgaaaa cagcagtcaa caaaaactgc ttggagatca
    19381 ttttgtgatc ctggctaaaa atgtacttcg taataaaatg ctccttgtgg tggtgatgcg
    19441 cgtgtgcatc atcgtgaaga gcgtgtgctt gtgttgctgc catttctatc agtattgaat
    19501 ggaatcgttt tttaattctg tgccactaca cttggtgtag cagttgtgtc ggtacttgtg
    19561 ctgtcgttgg ttggaacaat tgtatcttca ggttttgctg caactcctgc aaacggaagt
    19621 tgttcattgt accatttctt atactcttct tccttctcaa ccacaatttt catccacatg
    19681 ttattatgtg ccgctccgca aattttgtta caaaggagaa tgtaatcgaa cttgtcatca
    19741 ccggtgatgg tacgcatact atcagtggtt atagtaggac gaagtttcag acgtgtaacc
    19801 attccgggaa cggtattcat ttgcgaacgg aaatgcggca tataagcaga gtgaattacg
    19861 tcctgtgaac ggaaaaggaa ttcataatcc ttattcacaa caaggtgaat ctctttcaat
    19921 ttgattacgc gatcgtcggc tgcttgttga tcaatttcag gcgacattgt agaacgaagt
    19981 gcaattactt ttttgaggtg acgctgcatt ctttcctttt tcgctttcgt tttcgctctt
    20041 gcggcttcag tcatcacctc agaagggttg gatggtcggc cggactttgc ataaagcgaa
    20101 tccagatcaa caatcatttt ctccattcct gcaatcgttg catcgatgct tgcagtggtt
    20161 tgaacgccta atggattttc tccggtaaca agacgaaaat cgctgcgccc aagtaaatta
    20221 tcctcaccgg aataacgtgc gatccactcg aactgcttag aatacaattc aatctttacg
    20281 cgctctcctt cactgtcatc aaaggtggta atgttgttcc aggtctttaa cccaagaata
    20341 atgattactg caagaacagc tgcaggaact actgtccaaa gtaattcaag tttgttgttg
    20401 tggggataga aaaacgcttt gcgctccttg ttgtaggcat acttgcttgc aaaaacaaaa
    20461 agaagcgtgt tggtgatgaa gaacacaacg ataattaagt aaaagttgat gttcagcagc
    20521 cagtcagttt ctgctccctg tgcagaagcg gcaaccggta atccaccgtt accatatttt
    20581 gcaagaaggt aaatgaagga gacgtagagg ctcactatga ataaaatcat caggtttgcg
    20641 ttgagcttgg tgtctcctgc tgaaatatct tcttcacgct tgttccgcag ctgtgcggaa
    20701 agctcaagaa ccctcattaa ctgagcgatg gcgatgatcc ccagtaaaat gatcagaacg
    20761 atgataagct tggtcatgtc gacttatgta ttagagaacg gtttggatca gatgtggtga
    20821 tgaatacttt cttcgaggta agggtgattt acaggcatca aaggcgcttt ggtcaatgct
    20881 cttaaaacta ccattatgaa aactccaaga aaagctaaga acattcccaa ttcgagaaga
    20941 ccataatgct ggtgatcgaa catggtgccc ggcattacca agagataagt atcgtaccag
    21001 tgaccgaaga aaataatcat ggcgatgatg gtaagcagtt tgcggtgacg ctttgattcg
    21061 cggctcatca aaagaagcat tggcaaaata aagttgatcg caaacatcag gaagaattcc
    21121 gttttgtaat gttcaatacg gaactggaaa taagtgactt cttcaggtat gttggaatac
    21181 caaatcaaca taaactggaa gaaccagagg taactccata ggaaagagat tgcaaaaacc
    21241 catttaccca aatcatgaat atggctttcg tttactgctt tcaaatgacc ttgtccctga
    21301 agatagaata ccagcatcag aaccatgata attccactga tccacattcc ggagaagaca
    21361 taccatccat aaagtgtaga gaaccagtgg gtgtcgattg acatcagcca atcccatgac
    21421 atggtagagg agaatacagc gaaaaatacg aggaacaagg ctccctgacg gtagttctta
    21481 aagtgaatat ctgttccgcc tacctgatct tcgaggagtg agcgcttacg gaatgcacgc
    21541 gcaaaaagaa taaatgttgc taagtataca agtgtgcgaa cccaaaagaa aggaatgtta
    21601 aggtatgctg ttttatttgc aataatttca tcatgggcaa ctgcttcagg atccatccag
    21661 tgataaaggt gattcaagtg caaggaagaa gcagcaaaca caatcagcag cactagtgcg
    21721 ccaatcggaa tgtacgccat tactgcttcg taaacacgct tggttacaac tgcccacccg
    21781 gattcagtag catattgaag agcgaccata aacagaccaa ttaaggaaac tccaaaaaag
    21841 aagaatccat tcaccagtag gttagaccat agcttttgtt ggctatttcc agaatggtcc
    21901 ataaagaacc cgattagagt tccggccaac ccgattccta tgagcgcata ggagaagttc
    21961 tttgcccgtt tcgagatcgt gaattccatg cttagcgtat tgttgtttgc gtgaattact
    22021 taattgttgt tgaatcggcg acgatcgcag gataatttcc atcattctgc aatgagcgga
    22081 tataatggat tacagtccaa cgttcttctt tatttatctg accggcatgt gagcccataa
    22141 ttccctttcc gtaggtaatt gaatggaaca ttttaccgtc aggcagagca gtaaggttag
    22201 ctgacttata atcaggaaca cctgagatct tgccgttatt aactatagat cctttaccat
    22261 cacctttttc accgtggcaa tgcgtacaaa actgtgtgta aatacgttca ccttttttca
    22321 ggataacatc tttattttcg ctattgattg caatcgggtt gctgagtact gctgactttt
    22381 catacatatc agcctcttct tcaaccgaac gtggatgaaa tgggtaaggg aaattcagtg
    22441 ctgctttgct ggcatcttca gaaaaaggta tagtaccttt tgccggaatg cgtgcagact
    22501 gtacatcagg gtgactgtaa tccacgtagg cctcaacaga tggagaacgg tacatgtcag
    22561 gcatgtattc gtaacccggg ctgttagggt ctttggaaca ggcaaccagt cccaggacca
    22621 tcaacgatag tccgaacttt acaatggtgg ttgagcgatt catacgcatt aggcttctct
    22681 tacttcaagt tcaatgactc cggtttcttt aagcatagat tgcaattcct cagcagaaaa
    22741 cttcccgttt tcagtggtat gcagttccat tacaaactta tcatccgtag tacgcggatc
    22801 cgggttacgg gccggcatac cgggaagggt tttgttacgc aggaggtagg taatagccat
    22861 cccgtgagca gcacaaaata ccgtaaactc aaacgttacc ggtacaaatg caggaaggtt
    22921 ttcaatcaac gaaaaacttg gtttaccacc aatgttcatc ggccagtcaa caatcatgaa
    22981 gtaacgaata cccacaattg cgagcagggt tccggttaat ccataaagga aagcggcgat
    23041 acccaaacgt gtatgctgca ccccgataac aggatcaatt ccgtgaatag gaaacggtga
    23101 ataaacatcg ttaacgtgta caccttttgc gacgagcttc tttgcgccct ccaggaggac
    23161 catatcgtcg tcgtacattg cgtaaattac cttgttggcc atctcagctt tttattgatg
    23221 ttaatgatgg ttttctgtgt tacggtattt ctcacctgaa accctcagga tcgatttcac
    23281 ctcattgagc gcaagtaccg ggaagaaacg tgcgaacaac aagaagagaa cgaagaaaat
    23341 tccgatggta ccgatgaaaa tcccgatatc tacaaacgtt gggtagaaca tgctccacga
    23401 agaagggatg tagtcgcggt gaagtgatgt tacaataatt acaaatcgct caaaccacat
    23461 accgatgttt actacaatcg acatcacaaa agtgaaggtc agattagtac gtagcttttt
    23521 aaaccagaac agctgcggag agattacgtt acaagtcatc atactccagt atgcccatgc
    23581 ataagggccg gagaaacggt tgatgaaggc ataactttca tactcaacac ctgagtacca
    23641 tgacacaaaa agctctgtga ggtatgcaac accaacaata gaaccagtaa cgataattac
    23701 aatgttcatg tattcgatgt gcttattggt aatatagttt tcgaggtgca ttaccttacg
    23761 cataatcagc aaaagggtga gtaccatcgc aaaaccggaa aataccgcac ctgatacgaa
    23821 gtaaggaggg aagatcgtag tatgccatcc gggaataacc gaagtagcaa agtcggtgga
    23881 tacaattgag tgtacagaga atacgagtgg tgtggctaat cctgcaagaa caagcgaaac
    23941 gatctcaaaa cgattccatg cttttgcatt tcctgtccac ccaaaagcga gaatgccata
    24001 gacctttttc gccagaggct ttgtcatacg gtcgcggata gtagcaaaat caggaataag
    24061 tccgatgtac cagaatacaa gcgaaactgt aaagtacgtg gagatcgcga atacgtccca
    24121 aagaagcggt gagttaaagt tcacccacaa cgatccgaac tgatttggta aagggaacac
    24181 ccaataagca acccaaatac gtcccatgtg aatccccggg aatgtggctg ccatgattac
    24241 agcgaaaatg gtcattgctt cagcagagcg gttgatcgcc attctccaac gctgacggaa
    24301 cagtagaagt acagccgaga taagcgtacc agcgtgaccg atacctaccc accatacgaa
    24361 gttggtgata tcccatgccc aaccaacggt tttgttcaga ccccatgttc cgatacctgt
    24421 acctaacaga taaaggatac aaccaactcc ccagagtgct actacaccgg caatggctag
    24481 taatacatac caggctttgg gtgctttgct ctcgataggc cgacaaatat cctccgtgat
    24541 gtcatggtag gtcttgtctc caagaatgag cggtgtccgt attgcagctt ccttttgcat
    24601 tatgcggatg ttttgcgatt agtgactgtg ttcttcgtga ttctctgact tagcagcaac
    24661 aggtgctcct tcaatattgc ggactttaac ctggtaccaa atattaggct taactcccac
    24721 atcttccaat gcctgataag cacgttctga agagctttcc ttgcggatca tggaatcggt
    24781 atcgttccag tcaccgaaag taattgcatg tgtaggacaa gcctctgcac aagctgttga
    24841 ggcatcgcca tccaccagtt tgcggctttc tttcttcgcg gtaagcttac cggcctgaat
    24901 acgctgcacg cacattgagc acttctccat aacacctcgg gtacgaacga ccacatcagg
    24961 gttaagaaca agacgaccca tatcgctttg cgcaggattg aaagaagtaa atttcttata
    25021 agaaggataa ttgaaccagt tgaaacgacg aaccttataa ggacagttgt ttgcacagta
    25081 gcgggtaccg atacaacggt tataagccat ctggttcaga ccctcgttac tgtgagttgt
    25141 tgcagcaaca ggacaaaccg tttcgcaagg agcatggtta cagtggtgac acatcatcgg
    25201 catgtaaacc acttccggac tatcggctgg attttccatt tgactgtaca tgctgatagc
    25261 gccaacacct tcttcttttg cacgttgttt ggtcatgtca gaagaatagt agcggtcgat
    25321 acgcatccaa tgcatttcgc gaccacgacg aacttcgtct ttacctacta caggtacatt
    25381 attctctgca ttacacgcaa ccacacagtt tccgcaaccg atacaggtat tgagatcaat
    25441 gctcattccc caacggtggc caatatgttc aaccggatgt tcgttccaga gatctaattc
    25501 gccaattggc ttatctactt gctttccacc ttcgtggtac gcaatggtaa gcggcttgtt
    25561 gtaggcgttt ctatcacctg ccttaaacac accccaggtg gtttccttca caacggaagt
    25621 acgtcccatt actgtttgat gcgtttgcgt tgaagcaagt gcgtaagtat ttccggttcc
    25681 tgaaacagaa gctttataag cacggtagga aaggttacct ccgcttacaa caacaaaggg
    25741 atatacgttc ttaccaacag aaacatgctt tccgtcggac attgtaggtt ctccgtattg
    25801 ctcatattga taagcagcct taccaatatt ctctccgttg gcaccacgtc cgtaacctaa
    25861 agcaatacca attgttcctt tagcctgacc gggtgatgga caaaccggaa gcgtaacttc
    25921 acctaaacct tcgattgtta ctgtagcaac atcagcaggc cattcctggc ctagtttggt
    25981 attgtatttt ccttccatat cggaaggagc cattgtaatg taattatccc aggtcacctt
    26041 ggtgataggg tctggtaatt cctgaagcca tgggttggtt gcatgagaac cattacccat
    26101 tccggtattc tgatagaata caacttcgaa ttcaccgccg gaaattttgc tgaggtcagc
    26161 ggcggctttg tttacatcag cagcaaattc tacagccgat gcaggttttg ctgccatatt
    26221 tcctgaaccg ttatgaacgt tccagttcca atattcttcg aatgtggcaa tctccgtttg
    26281 agttgggaat ccataagctt cccagttttt acggatcaca ttgtaatacg tgtcattatc
    26341 cttatcacca cgtgtagcta aaccggcaaa caccagcatg ctttcctgca tgttgcgtgt
    26401 ttcgtaaagc ggacgaattg caggctgtgc aattgcgtaa tgattagcct taggattgag
    26461 atcattccaa ctttcgagga aatgatgatc aggacaaaca taggcacact tcgatgcagt
    26521 ttcatctgct actaaagaga aggaaactga aagatccatc ttagcaatag cctcagcaaa
    26581 tgcattgcca tcacgaagtg aataaacagg attaatgttg ccatcaataa ggattgcttt
    26641 gatctttcct gcagttacat ctttcaccaa ctcttcaacc tgacgatcgt tagctgcgaa
    26701 aagattaacc ggattattga tgttgatggt ggtttcgtaa ttaccaagct tgtggttaat
    26761 agcagccaca atggtttgaa cattaacatc attcgaacca gaaacaacca aagaagcgcc
    26821 ggctgatttc ttaagttcgg caccggcagc agcagttaat tcaaggattt cagcaggcac
    26881 attcccaatt gaagatcctg ttacatgctt atacagtgca gcagcaacca atccttcctg
    26941 agaaggcttg atggggacac gaacatcagc atttgaaccg gtaacagaca tgatcgattc
    27001 gaattggaag tgacgagaca tccaatcgtt ttcgggacga cggttttgtg cgtattgtgt
    27061 tacatattca ttgtgaagca gccagtttgc aaggaaatcg gcagaaatac ttacaatggt
    27121 tttagctttt gtaaagtcaa gatcaggtat tacattctgc ccaaatgaag cctggtgagc
    27181 atctctgata gcagaataag agattggatc gtactgtaca tgaacaatgt ttgcagcggc
    27241 ttcggttccc tgtgcggtac cgccaagttt tgcaataaca tcagcaacaa cagtagaagt
    27301 agaaggagaa atcactgttc cggtaaggat gcggatttgt ccacctttag atgcgatatc
    27361 tgttaatttt ttagagattt ctccatccac ctgaacccaa tcggcaggag cacctgcggc
    27421 catgggaccg gtaagacgag cagaatcgta taaagaaaga acagaagccg ttacgcgagc
    27481 attggctgca ccctttgtaa ttccgtgttt gcggttaggc ttaataaaga tagggcgacc
    27541 ctcacgcgtt tttaccagaa tgttggcaaa gtcgtttcca tcataatagg tagaagcata
    27601 ccatgtagcc tcacccggag taatatcctc cggtttggtt acataaggaa tggacttaat
    27661 caccggcgct tcacaggcag caagtgttgc cgctgttacc gagaatccga gaaatttgag
    27721 gaaatcgcgg cgcgacgtgg atgactcctt gagtttatca tctcccagga atgcatccat
    27781 gggcaattcc tcagggaatt cattgtcgcg gtttctgatg aactcgggtg cgttttcgag
    27841 ttcttcgatt cctttccaat attgcttgtt catgctcata atgtgctccg aaaagttgat
    27901 taatagtgac acttagcaca ctcccagcca ccgagttctt ctacggtgat cttttcgtct
    27961 gcaaggtgct tttgataaag tgccttattg ttcatcaggc gctgtttcag ctccgtgtaa
    28021 taaccatttc catccatctt cacttctgcc ttgttatggc attcgataca ccaacccata
    28081 gttagtgttg cgcgatctac cttaacatta ccctcaattg cattcaactc ttcatgaggc
    28141 ataacacgtg caacagtttc atttttcatt tcaccgtgac actgtttgca atccaactgg
    28201 ccggctacaa cgtgctgctg gtgtgagaaa taaacgtgat cgggcaggtt gtgaactttc
    28261 acccagcgaa ttggctctgt tttaccagta taactcatgg tagcaggatc aaaaccaaca
    28321 gccttgtaaa tttttgcgat ttcctcttca cctgtaatgg ctcccttttg aattgccttg
    28381 tggcagttca tacacaccat aggagaagga atcattgcat gcttggattt ctcggtagtc
    28441 gagtggcaat actgacaagc aatctcattg tcgccagcgt gaattttgtg tgagaaagca
    28501 atgggttgtt cgggtttgta gttttcaaaa acaccaacat ccttaaggcg gtaccaacca
    28561 tcggacaatc caccaaaaat gaagaagagc gcgaacatgg aaaccagcac cttatggcgc
    28621 caagcccatg cacaccaggt ttgccaatag gtaaggtcag gaagttcatc tttgccttct
    28681 ttttcaagat tggcattcac caattgacga cgaactgatc cggcagaaat ggcgataacc
    28741 gtaaaaatga cacccagaat aacgatccac atccagcccc cttctttgct ttgggctccg
    28801 gcagtttcag ttgcgtttac agatacagca gccaccggct ctgtatagga ttctacatat
    28861 tcgaaaattg catcgatatc ttcattggtt actgcctgtg caggcattgg acttttatta
    28921 aaatccttga agagctgatt tgcatatgca tcacctgaag aaagaacttc attggaattc
    28981 ttgatccatt tgtaaaagtt ctcttctgat gagtttttct tccagcgctc acgtgcacct
    29041 ttcaaagcag gaccgaccat gcgcttttcg gggtggtgac aagcaccaca agcctggaaa
    29101 agagcctgac cgtcagccgc ctgaacggaa ttgacagtgg aaaaaccgag tagaaaatac
    29161 aggcatgccg tgatgacggc aaaactcctg aaagtgaatc ggaatggatt gttcatagat
    29221 cgcagtcgac taaggttaaa aaatgcttaa ccgagggcaa atttatcaga gaaaaagtat
    29281 cagcatctaa aattgtcaca aaattgtcag gggggctatt aatttataat tattctaaat
    29341 aaggccttgg aacgaccgtc tttactatca atcgcttaag tcggtcatcg catcgttcaa
    29401 tcttgaaaaa ttgcccataa aatacaaggc ttggcaattc gtcatgtggc aaggcttttg
    29461 ctatttttgc agcatgcgca tgtttctgat cctatttcta agtttagcct cattatcact
    29521 tatccactct caggaagttg agtcggactg gaagcttttc cgtccggttg gcgctattaa
    29581 agacactgtt aagcgtcagt ccaacggaac aagtattcaa cagcgaggtt cgttttttca
    29641 gttcagccaa caggggaaac agggtgatgt aaaagttagc aaggattcgc ggatagatgc
    29701 attgagcaca tttgtgggaa ctccgcaaaa tggaaattcc accgtaaaaa ttaaaggtta
    29761 ccgaattcag gcatcacttg accgggacaa aaaccgaatc aaccaattgc gtgcagaata
    29821 tctctcgcga ttctcagagc agccggctta cattgatttt ttagcgccta attttcgttt
    29881 gcgcatagga gatttcagaa gcaagctgga tgcacatgct tatctcgaca aaatcaaagc
    29941 aatatttcct gatgcggtaa tcgtaatgga tgatattgat ttgccccgat tgtaaactga
    30001 atcagaaacg atatccgatt cctaggaata gatcggttcc aaaaccgctg aacaagtttc
    30061 ctgaactggt accgtaaact acttttcctt catagaaagg aaatccaaat tttagtcggt
    30121 agccggctct aattccaatc tgtttgtatt cgtaaagcaa cacgggttcc atgtcgattg
    30181 acaggcttgc aaattttacc ggatactcaa tgcgctgagc gattccggaa taaaaataat
    30241 ctgcgaatcc actctggttt acaaatgaaa atgatggtcc tgccccaaag taacacatca
    30301 gtctgtgctt tttctttaca tatcctatca acaaaacctg gtattgtaaa gatgctgtaa
    30361 tcgccttgaa ttttaaagct agctgaacat tatcaggtgt accaaacggt gcggcaatag
    30421 aatcgtctgg gtcagtcatc cattccagct cctgtgttgg agaatatgat ccatccgggt
    30481 tgcggcgata aaaaacttct tcgttaactg atcctgcgtt tccgaatcct gctccgaaaa
    30541 caagaaagct gttctttttt aaatggtgct gatatcctat ccatggaagc aaagccttgt
    30601 tcccaatacg caagccaccg taaatacgat tattcgtagt tttcttctga tcgagattgc
    30661 taagtggttc ctgaccaaaa ccgtaaaggc tgaacaataa aagagaacaa agaaagagaa
    30721 caacgcgcat caatccagtt ttagaacggc cataaatgcc tgttggggca cctctactct
    30781 accaacctgg cgcatccgtt ttttaccttc tttttgtttc tccaaaagtt tacgtttacg
    30841 actgatatca ccaccataac atttggcggt aacatctttt ctcaacgctt taatagtttc
    30901 acgcgcaata atttttgctc cgatggcagc ttgaatgggt atttcaaatt gctgacgcgg
    30961 gattaactct ttaagctttt cgcacatctt ttttcctaac tcaaaggcgt gtgatcggtg
    31021 gatcaatgca gacaaagcat ctacctgttc attgttaagc aggatatcca ttttaacaag
    31081 gtcggactct ttgtacccga tcggataata atcgaaagaa gcatatcctc ttgatagtga
    31141 tttcaactta tcgtaaaagt cgaataccac ttcaccaaga ggtagctcga aggaaagttc
    31201 aactctatcg gtagttaaga aattctggtt gatgagaatt ccccttttcc ctatacagag
    31261 tgtcatgata ttgcctaagt attctgattt cgtaatgatc tgtgccttaa tgtagggctc
    31321 ctctaccttt gacactttac ttggttcggg taattgcgaa ggattatgca cttcggtgag
    31381 ttcccctttt aagtcgtaag ctttgtagga tacgttaggc accgtggtaa ttacagacat
    31441 attgaactcg cgttccaatc gttcctgaat aatttccata tggagcaagc caaggaatcc
    31501 gcaacgaaat ccgaatccaa gcgcggcaga agattcaggt tcaaaaacaa gagaagcgtc
    31561 attgagctga agtttttcca ttgcatcacg caattcatca aactcatcgg tatctaccgg
    31621 atatattcct gcgaaaacca ttggcttcac atcttcaaat ccctgaatag cctcagaaca
    31681 agggcgttca cggtgagtaa tggtatcgcc caccttaaca tctttggcat ttttaattcc
    31741 tgaaatcacg tagcctacat ctccgcaatt gatgtccttt ttggccacca tgtcgagttt
    31801 gagcgtgccg atctcatcag catcatattc cttacctgtg ttaaagaatt ttactttatc
    31861 gcccttgcgc agggtaccat tcacgatttt taccagcgca ataatacctc tgaaaatatt
    31921 aaactcagaa tcgaaaatca aggcttgcag tggtgcatta ggatcaccca ccggggcagg
    31981 aatccgttct accacagctt caagcacagc atccacacca attccagttt tacccgaagc
    32041 agacaaaata tcttcgcgac ggcaacccaa aaggtctact atctggtctt taacctcttc
    32101 cggcatagcg ccaggaagat cgattttatt cagcacagga ataatctcca gatcatgctc
    32161 cagggccaaa tacatattcg aaatcgtctg agcctgaaca ccttgcgctg catccaccaa
    32221 caataaagcg ccttcacagg cagcaattgc acgggatact tcataagaga aatcgacgtg
    32281 acccggagta tcaatgaggt tgagtatgaa tttttcgccc ttgtaattgt attccatctg
    32341 gatagcgtgg ctcttgatgg ttataccacg ttcacgttcc aggtccatat cgtcgagcgt
    32401 ctgctctgtc aattcacgat cactcaaggt gcccgtcacc tgcaacaaac gatcggccag
    32461 agtagattta ccatgatcga tatgcgcgat gatgcagaag tttcggatct ttttcatttc
    32521 cataaggctg caaaattaag caattagggc catcagatta cgcaataaac agggcaaaca
    32581 tcaaatctcc ttttcttttt taggtcctgc ccgcctatat ttgcggcctc attcacagta
    32641 tgaaagtcat tgaccacctc aaagatgcca agcgcacact attctctatc gagatccttc
    32701 ctccattaaa agggaagagt attcagtcaa tttttgacgg tatcgatcct cttttggaat
    32761 ttcagccatc ttttattgat gttacctatc accgtgaaga atacgtttac aaaaaacgtg
    32821 aaggcggtta ccttgaaaaa gtaagtatcc gaaagcgtcc gggcaccgtt ggtatctgtg
    32881 cagcaattat gaacaggtat aaaattgatg ctgttccgca catcatttgc ggaggatttt
    32941 caaaagaaga aacagaaaat gcgctgatcg atcttcagtt tctcggtatc gataatgttt
    33001 tggccttacg tggcgattcc atcaaaactg aacccggttt cgtgcctgat ccggatggac
    33061 accattatgc gcttgatctg gtgcagcaca tcgacaaaat gaacaatggg atttaccttg
    33121 aggatgatgt taaagacatg gcacccacca acttttgtat cggtattgca ggatatccgg
    33181 aaaagcacat ggaagctccc aatatggcaa gcgatatcaa atttctgaaa atgaaagtgg
    33241 atgccggtgc tgaatatatt gtgacacaga tgttttttga caataaaaaa tactttgaat
    33301 ttgttgatct gtgtcgtaaa aacggaatca atgttccgat cataccggga ttaaaaatct
    33361 tgacggggaa aaaacaactt accggtcttc ctaaaatttt ccatattgat ttgcctgaag
    33421 aactttacga aaacgttgaa aaggcgaaag acgataaaga ggcgaaggaa gttggaattg
    33481 actggtgtat tcgtcaatca aaagaactga tcgcgaaagg cgttccctgt ttacatttct
    33541 acacaatggg gcttagcgac tcaacccgac gcgttgccaa agaggtattc tgatcaggcc
    33601 cataccttgg tgccttcgga acaattttta caaatatcta tttgatcccg gccggataat
    33661 accgcatttc tgaactgatt gtaggcaggc gaagtccaat tcgatctgaa actttcctgt
    33721 tttagatcac caagtcgata ctcagcatct ttatcaaagc aacagggaac aagtttgccg
    33781 tcccaggtaa tcacagatga actccacatc ctccagcaat gatcttccat tttatttttt
    33841 accgaccaag atccgtcctt tttcttcttg tacctggagt atttttcgtt ttcaggaata
    33901 agaggatttc cattttcgaa attataaact tgcgctgttt tcaaccgaac ttcattcact
    33961 cccagctcct ttgccaattg aaagacctca tcaacctgat gttcattagg gcgtacaacc
    34021 aaaaactgaa agatgatatg tggagtagac gatcttaatt cctttttcca gcgaataata
    34081 ttttttgtgc cctctatcac tttgtcgaga gatccgttta cccggtattg ttcgtatgta
    34141 tcctgcgatg tgccgtctat agaaatgatc aaacgatcga gaccgctttc caccgttgac
    34201 tttgcttttt cgtccgataa aaagtgtgca ttggtagagg tagatgtgta aattcctttg
    34261 cttttggcat agtgcaccag gtcgagaaac tgaggatgga tatagggctc tc
//
LOCUS       DAOMYG010000002        26567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_666_length_26567_cov_10.378998, whole genome shotgun sequence.
ACCESSION   DAOMYG010000002 DAOMYG010000000
VERSION     DAOMYG010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 26567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26567
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_666_length_26567_cov_10.378998"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..562)
                     /locus_tag="PK610_00130"
     CDS             complement(119..562)
                     /locus_tag="PK610_00130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ34357.1"
                     /translation="MFHILPATHTDLPAVHQLIEQLEECSIPFEEFAPVYLRNCAMQD
                     IYYFVGEENGIIVGFVSLHIQYLLHHFAPVAEVQEICISDVCRGKGYGKMLLDHVTQL
                     ARERDCDLIELCTNKKRTDAHRFYDREGWKQSHFKYTFPFRKEYL"
     gene            646..1029
                     /locus_tag="PK610_00135"
     CDS             646..1029
                     /locus_tag="PK610_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34358.1"
                     /translation="MKRDEHLLNVRPRLDVEDDLSLPEEVFQNEVIRPVIKFQSEALF
                     AMMNEYIRRLSPDFRTYDLVRKRKFISSAIQKDHTLHHQLIGMVVACFTLEELQHFLQ
                     QRSEHSKRISQILTERFCSQAEKLG"
     gene            complement(1077..1781)
                     /locus_tag="PK610_00140"
     CDS             complement(1077..1781)
                     /locus_tag="PK610_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HRJ34359.1"
                     /translation="MKSLYLFIDLFSLLGPLVLSFDKKVAFYKNWKALFPAIAIMMLV
                     FIPWDVVKTAKEVWGFNEDYLLGITIINLPLEEWLFFICIPYACIFIYECLNAYVKTD
                     LLAKVYKPLLTILAIVLIATGLVNYVKWYPATAFPYAGVLLLLMLYVVKSPYMSRFFL
                     AYLVTLIPFLLVNGMLTGSWIAEPIVWYNSEEIFNIRIGTIPVEDTIYNLGMLGTVVV
                     VYEYFKSVFTTKAQGH"
     gene            complement(1778..2257)
                     /locus_tag="PK610_00145"
     CDS             complement(1778..2257)
                     /locus_tag="PK610_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRJ34360.1"
                     /translation="MIYALTVLATFCVMEFMAWFMHKFIMHGFMWYFHEDHHQPIPKL
                     FGFFERNDIFFLIFAVPSWLCIMLGMMYGNYISVSIGAGIAVYGFAYFLVHEVFIHRR
                     MSWFKNSDNVYLRAIRKAHKVHHKHLGKEDGECFGMLIVPLKYFREARESMRQKQKA"
     gene            complement(2277..2915)
                     /locus_tag="PK610_00150"
     CDS             complement(2277..2915)
                     /locus_tag="PK610_00150"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HRJ34361.1"
                     /translation="MNDSFSPLRLWIAIAVLVIFYAVGFWGFATQNQLEFASLTWVNL
                     VMSGVFVFFPLRKLSGSELLVMISIFVLSFVVEMVGVMTGALFGEYAYGANLGWKMYE
                     VPIVIGLNWLMLVYATQQVASVLFSHWLLIAFFGALLMTGMDFLIEPVAMKLDYWQWK
                     DDTIPFRNYLSWFIFGFIFQVLMVKVFRPKMNPAAICLFVLQAVFFWWLGTR"
     gene            complement(2912..4387)
                     /gene="crtI"
                     /locus_tag="PK610_00155"
     CDS             complement(2912..4387)
                     /gene="crtI"
                     /locus_tag="PK610_00155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HRJ34362.1"
                     /translation="MKKALIIGSGIGGLATAVRLAVKGYAVEVFEASDHPGGKLSEKN
                     LGGFRFDTGPSLFTLPALVDELFLLAGKDPAQHFSYTRIPESCRYFWEDGFRLAAGSQ
                     PEDFAAACAKTFSVPAEDILRYFAECKSMYDLTEGVFLERSLHRAKNYLRKDVLRGVM
                     NAWRLHLFRTMHQVHRKRMKSPHLVQLFDRFATYNGSDPYQAPGILSMIPHLEHNLGT
                     FFPKGGMIDIPNVIYKLALELGVKFHFNAPVETIITQNKKASGIRVNGKEITGDVVIS
                     NMDVVPTYRRLLPNEKAPEKSLQQERSSSALIFYWGISRQFPELGLHNIFFSADYEKE
                     FRMLFKENSVTDDPTVYIHISSKYCKTDAPDGKENWFILVNVPGNNGQDWDALIPQIR
                     ANTIAKLSRMLNTDVASLIEQEDRWEPRTIERDTFSYQGSLYGSASNNRYAAFLRHPN
                     FSRRIPNLYFCGGSVHPGGGIPLCLRSAKIAADLILEDQSL"
     gene            complement(4421..5257)
                     /locus_tag="PK610_00160"
     CDS             complement(4421..5257)
                     /locus_tag="PK610_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34363.1"
                     /translation="MNRLSVFLGLFLLFSSFSGQSQNTEIVRKGRFFFGWGYNRAQYS
                     SSDIHLWGPGYDFVLDNVLASDRPEAFNLRVYANPVHLARPQYNFKIGYYLTDKISIS
                     LLDDHMKYVMRNWQPVLMEGYITEAASSQFAGQYNDSVMMLSPDFLYFEHTNGLNLLG
                     AEIDFMDSLWSSRKYKLKLSMEAGGGIGVLYPRSDITVFGNPGANLFHTAGYGLSAHI
                     GLRLDMGKHFFLRATVKPGWINLPNILTYSDRAHGASQKFFFLEEYVQLCYILPSLKR
                     KK"
     gene            5340..5540
                     /locus_tag="PK610_00165"
     CDS             5340..5540
                     /locus_tag="PK610_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRJ34364.1"
                     /translation="MPTGTVKFYNRKNKFGFIKVDDTGAEIYVRQSGIVDPINEGDRV
                     EFEMEEHPKGPIAVKVKKLAAE"
     gene            5618..6703
                     /locus_tag="PK610_00170"
     CDS             5618..6703
                     /locus_tag="PK610_00170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ34365.1"
                     /translation="MKADRHILVLCMHHIDRAPNQRFRIEQYVPYLRENGVELTFSPL
                     LSKEDDAVFYRPGNLFKKIQIVLRSIRQRRRDLQRASQFDAVLIIREALFIGPPRIER
                     KLRKKGLTVIYDFDDAIWKLDVSRANRWFGFLKFPGKTAAIIRLADKVIAGNEYLADY
                     AKAFNPNVHIIPTTVETRTFLPGKKDASSPVVIGWSGSLTTIKHFRIAVPVLKKLREH
                     YGDRIRFKVIGHAGFSEEELGIRGTAWSSERELEELQEIDIGIMPLPDDEWSKGKCGL
                     KGLVYMSIGIPSVMSPVGVNTQIIRHGENGFLAGTEAEWFEILSLLIESPELRKQIGS
                     SGRQTVVENYSVLANRENYLNILGKSS"
     gene            6791..7891
                     /gene="gcvT"
                     /locus_tag="PK610_00175"
     CDS             6791..7891
                     /gene="gcvT"
                     /locus_tag="PK610_00175"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRJ34366.1"
                     /translation="METTTALKNTALTDKHIALGAKMVPFAGYNMPVSYSGLNDEHAT
                     VREAVGMFDVSHMGEFILRGPNALDLIQRVTSNDASVLVDGKAQYSCLPNDKGGIVDD
                     LIVYKIADEHYMLVVNASNMEKDWNWISKHNTKGVDMKNISDDTSLLAVQGPKAMATL
                     QKLTDVKLSEIPYYAFRIGTFNGVNEVVISNTGYTGAGGFELYFPNAHAEKIWDAILE
                     AGKEFGIKPIGLGARDTLRLEMGFCLYGNDIDDTTSPLEAGLGWITKFTKEFTNSKAI
                     AEQKEKGVSRKLVGFEMIDRGIPRHDYEIADAAGNIIGKVTSGTQAPTLQKGIGMGYV
                     STSFASPDSEIFVKVRDKALKAKVVKLPFLKK"
     gene            7910..8632
                     /locus_tag="PK610_00180"
     CDS             7910..8632
                     /locus_tag="PK610_00180"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HRJ34367.1"
                     /translation="MSQATVDACFSPYLYPVYRNDDNIVVIIDILRATSAICTAFEHG
                     VEKIIPVATVDEAREYKAKGFLAGAERDAVPVEGFDFGNSPFSYMGEHIKGQTIVLTT
                     TNGTQAINAAKDAHKVIIGAFTNITAVCNYLKSQDRNVMLLCSGWKNKFNLEDALFAG
                     AVTEELSKHPELFKLQDAALALKYLYQQAMEDPHKFLHNASHKERLAKLGLKEDIKYC
                     LQNDLTKEIPVLENGALVRLRY"
     gene            complement(8709..10571)
                     /locus_tag="PK610_00185"
     CDS             complement(8709..10571)
                     /locus_tag="PK610_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRJ34368.1"
                     /translation="MKRKIEILAPAKNLIQGMAAINAGADAVYIGAEAFGARSNATNS
                     VDDIAEMVEYAHLFKAKVFVVINTILYDNELEACRKLIHKLYAIGVDALIVQDMAILE
                     MDLPPIVIHASTQANNRDPKHVKFLAEAGIQRVVLARELNLDQIREIHEATDVELEFF
                     VSGALCVSFSGNCYMSIAGGERSANRGSCAQNCRLPYQLIDGNGTTLIANSHLLSIKD
                     LDLSDQLPQLIEAGISSFKIEGRLKDMVYVKNNTSYLRKKLDAFLENNPHFEKASSGR
                     TFYNFEAKLDRSFNRGYTDYFVNKRTERIGSWESPKSQGQLIGKLIETKANGYVIENS
                     DLLNNGDGLYFINENGEADGAQINIIINDVVVPNVFKPLAPGTVIYRNSDAEFNRLVE
                     REDSVIRKIGVQLTLKDTTQGFLLEACDEDGHKAIVEAVLNKELAKSAESAEPNIKKN
                     LAKAGNTPFVVDEITIEFSSNWFLPISKVNEMRRDVLDQLMDVRLKEYVREEHQHVKT
                     DHPYPVEKLDFTYNVSNKLARAFYKRHGVTEIEKAFELQWDPGKSRVMVTKYCVKYEL
                     GKCPRFQRETMGEKLTEPLTLKHGTNEYKLKFNCKPCEMEIWEKDAELEFEEED"
     gene            10791..11792
                     /locus_tag="PK610_00190"
     CDS             10791..11792
                     /locus_tag="PK610_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HRJ34369.1"
                     /translation="MRRPILLFVLVLLLMPVIGFRMMGHSSWGFYAHKRINRMAVFTL
                     PPGVFGFYKDNIEFVTDHAVDPDKRRYAVTGEAQRHYIDIDHYKIEEEDVFVTVPKKW
                     KDAIKKYTEDTLQAYGIVPWHIEVMMFRLTKAFQEENYDQILKLSADLGHYVGDAHVP
                     LHTTENYNGQLTGQKGIHGFWESRIPELFAEEYDYFVGKAYYIEEPLEFAWQAVKESH
                     HAVDSVLNFEKILSKQFPEDRKYSYETRGNVTVKVYSQEFTKAYSDMMAGMVERRMRK
                     TIIAVGSLWYTAWVNAGKPDLNKHKNKQISPEMQQELQAEDKLHQEGKIKGRGCDHN"
     gene            complement(11921..13549)
                     /gene="lnt"
                     /locus_tag="PK610_00195"
     CDS             complement(11921..13549)
                     /gene="lnt"
                     /locus_tag="PK610_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRJ34370.1"
                     /translation="MSKLPLIVYSTLTALLMALAWPFIGSLWPVVFIALVPMLLLEEH
                     VFRKGYRPRKVFLHAYWFFLVFNCATTWWIWYASAGGAVMAFFFNALLMAIPFWLFHV
                     TRRRVGSREGIIAFFLNWLAFEYLHYSWELSWPWLSFGNVFAMVPSVVQWYEITGVTG
                     GTLWILVLNFLFFRIVRDVFWLQKQSMKEQLSRFIFIALLLFTPILISLKMYSSCSDK
                     GEPVDIVIVQPNIDPYNEKFGGLTMKAQFDIYADLARKSLDENVEYLLGPETQIPFSV
                     NEDAIDSNTAVKAFRNLCSDYPQLKIVTGMSSYTVFREGSRKSPTAKPTSEPGYVYDH
                     FNASLQIENNAAVHVYHKMKLVLGVEKVPFSGWFPFLEKLALDMGGSSGSMGSETAPK
                     VFTAQKDPRHVVVPAICYESIYGEHIAEFVRLGGDLIFVITNDGWWEDTPGYKQHMSY
                     SRLRAIENRRSIARCANTGISCFINQRGDVVQQTKWWEPAVLRGVVFANTEQTWFTRH
                     GDFIGRSALFVALLLTFYTLTRMLMGKTASKKEI"
     gene            13695..16514
                     /locus_tag="PK610_00200"
     CDS             13695..16514
                     /locus_tag="PK610_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRJ34371.1"
                     /translation="MAALLLLPISLQAQTAKKKIDHTVFDSWKLVRAANYSSDGKVIT
                     YEIQPQSGDGYLYVYRIQEGKLDSIARGYEARFIDGSNLVLCKVKPAFAATRKAKLAK
                     KKEEDLPKDSVALYHVEQGKVIAGTVMVKSISGKEESKWYAVHHSKMPEPAATGKKKK
                     KKKPAPEIKSEGTWLELVNTTHVWKKADPKAKKPKNAAISQYRFQHVTEYTLSKKGER
                     FAFVQQRKVGKTDSAYITVFDTQNGIPTRIYSVAGFCKALSFDEKGAQLAFLSSTDTS
                     KNKVYDLWYWKNGHTAPSLLVDAANTAMPKDWCVSENGTLRFSENGSRLYFGTAEKPQ
                     KEIKDTLTEDEKYKLDVWSWTDTRIQPEQLKQLERDKKKNYLAVYHLSTSKMVQLADK
                     EVEFVRTNQEGNGTLALGITEAPYERSKTWEYPIASDYYSIDINTGEKKLILKDQKFY
                     CDLSNAGNYLLYYKRKDDNWYALEIASGKTINLTEKITEDFFDDDNGNPSEEDAQGIA
                     GWSENDGVVYLNGKYDIWKVDPRDPSKPVCLTKNFGRETKTVFRYTELNPEAKFISEN
                     DMLLHSFNERTKKAGYYRVNLDGKTPAVLLESDHKYPYILKSKNSDKTVFARMNFLEC
                     PDLWISDMNLQNIRRISNSNPQQSEYNWGTVRLTNWKTPKGRQLDGLLYLPEDFDSTK
                     KYPLLVYFYEKYADDIHNYYSPRPSASIINPSEYCSNGYVVFIPDILYNEGTPGEDAY
                     DCIISGTNHIVSKGFINKERMGLQGQSWGGYQTAYLVTQTNMYRAAMAGAPVSNMTSA
                     YGGIRWGTGLSRTFQYEKGQSRLGVTLWEDRERYIKNSPLFFADKVNTPLLIMHNDRD
                     GAVPWYQGIEYFNALRRLDKPVWMLNYNDDDHNLTRRANQKDLSIRMRQFFDHYLLDA
                     PMPVWMKDGLPAIDKGKRTGYELEK"
     gene            16832..20170
                     /locus_tag="PK610_00205"
     CDS             16832..20170
                     /locus_tag="PK610_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ34372.1"
                     /translation="MKRLFTKVVFALLMLLLNNSMHAQNINFSGNISTNTIWSSAAID
                     TVKITGNVTIDSLVTLTIEPGIVVDFTGYYYLNVKGTLRAVGTVTDSIRFTTRDTLGF
                     SLNNHTGWAGIRIHARNNTNDTSRFSYCVVEFGKANGTSGLPNASHGGGLYFYQSWKI
                     ILNNSSIRNNYAQTAGGGLYEYYSYVNTSNAQTIKNCVFKNNKSANTGGAIYTQRGLE
                     LAIENCTFIRNTAASSGGAIFVENTSTSRAKIGNCRFSYNNAIGGGAVSVYGTLSGGY
                     INDCTLNNNTAQNGGAIQVSQALCDIKNCVIVNNTATSNGGGIYFNNVFSNFSGTNAI
                     INNTIAHNTANLGGGAYIFDNSSGADLYINKTIFYNNVSSNAGAFNNDVYLEQFLGSN
                     QTRFYGCSFTATLANTTGNPNNIGSLLNTNPQFANPSAGIGAAFEGFTGRNWTLNFCT
                     SPCIDGTNDNVACACFPATDRNGNPRKTNNFVDIGAYESDGFITQPLSVSACVGDAIN
                     FSYDYFFNTGSTPNSYWEYSTNGGANYTSIASASNTYAYTTAALLSDHNVLVRVRHDY
                     GCREVYSTVATISIFTPLNITANTTASSVCLGTPVTLSGSGAVSYSWNNGVTNGIAFT
                     PASTNTYTVVGTDGNGCATNPATVTVTVNNNPTVSITASETSICNGDDVTLSGNGALS
                     YLWNNGVVNGISFIPASTTTYTVSGTDANSCVGTATVTVTVNQPTISPIASSTGVCEG
                     NAVTLSGSGAVSYTWSHGVLDGVSFVPAATATYTVTGEDSDGCTNTANITVTVHSLPV
                     ISINANPATVCAGTATTLTASGGVSYQWMPGSLSGTSVSVSPSVQTTYTVIGTDANNC
                     SSDETIEIIVLDLPNADIGPASLTVCDGDQLNFSLSSTAGVSTYSIVTAFHNSVDAPL
                     SFVYDGNNPPSLVQCNSTGTNGCTASQVVQLINNSILNLSQNIPTGTDPTYTFHALFI
                     PANVDNWMWDFGDGTTLSGNTDPVHTFTANGNYDVCLIATNSCDSVAACFNITINSVG
                     IEELSRTIKVYPNLTEGWVFVESTMENEVMMEVLDVSGRVVYAASLFDSRKSIDMSPW
                     QNGVYFIRLTQANQVHVVKVVLQK"
     gene            complement(20301..20468)
                     /locus_tag="PK610_00210"
     CDS             complement(20301..20468)
                     /locus_tag="PK610_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34373.1"
                     /translation="MSRVLFIKIKMMLNVVLDVLDKIGDYINTRWAWFFTNGNKNQDT
                     TSDNSQLTESF"
     gene            complement(20480..20752)
                     /locus_tag="PK610_00215"
     CDS             complement(20480..20752)
                     /locus_tag="PK610_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34374.1"
                     /translation="MSQNTRQQTSTGKMVISVNDLRDNEFAGVRLSTDEKQALSNYDH
                     YRISYLNSSENEEEFSRRYIEMQAKANLSPYTEFLKPPYSDHSLNS"
     gene            complement(21133..21726)
                     /locus_tag="PK610_00220"
     CDS             complement(21133..21726)
                     /locus_tag="PK610_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34375.1"
                     /translation="MFLVLHSAAESEMNDRIITLNNDTIYCYIPNNPKQVFREPRKTV
                     VYGYDYVVAKFAGDSVRFYNPGSIKGYIKKVTIKGHEQTEWYFSDSVEVGYNVWFQKR
                     HYKRPLFLRRLVNGSPFQLWFFEDYDVPSTNDRIFVLRDLPHKKESYFLRVRQLWSII
                     GTWSGCNPKDPRYKHWFTGPQYVVMDYNKSHHTAGKP"
     gene            complement(21935..22351)
                     /locus_tag="PK610_00225"
     CDS             complement(21935..22351)
                     /locus_tag="PK610_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRJ34376.1"
                     /translation="MSIVKVTVEAKIEQPVEKIWEFYNEPQHITQWNFASPDWHCPSA
                     QSDLRVGGKYISRMEARDGSMGFDFEAIFDEVDLHKKITYTMVDGRKADIFFQPSGNT
                     SNITIVFDAESENSPELQKSGWQAILNNFKTYCEGK"
     gene            complement(22378..22755)
                     /locus_tag="PK610_00230"
     CDS             complement(22378..22755)
                     /locus_tag="PK610_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HRJ34377.1"
                     /translation="MKNKVLNVVSALFGLLLLNGGLNKFLNYMPVPADLPQELIKDSM
                     AIMEISWLMPLVGFAEIVGGMMILFPKTRALGALIVFPVMVGVLLTHITVAPEGMPIA
                     IVIWIILSWIIWENRKKYLALLN"
     gene            complement(22764..23726)
                     /locus_tag="PK610_00235"
     CDS             complement(22764..23726)
                     /locus_tag="PK610_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRJ34378.1"
                     /translation="MKKAILFDFTADKTNNTIHVKRSFHAPIDLVWAAWTESDILDQW
                     WAPKPYRSVTKSMKFEEGGRWHYYMQGPEGDIHWCLFDYEKILPQQFYSGIDAFCDEH
                     AVMSASKPRVKWENGFSANDDSTVVECLLKFETLEDLEAIITMGFKEGFTAGLANLDD
                     YIAAQFYLRKDKKTDNAVRVSSYLNFDGKTEEAFHFYKSIFKTEFVKGIQRLGDFPPQ
                     EGQPPLAEEVKKMVLHVQLPITGGHILMGTDAPKEMGFTVTPGNNMHIHIEPESREEA
                     DRLFHALSEGGTVEIPIQDMFFGAYFGNFMDKYGINWMINYQPK"
     gene            complement(23723..24049)
                     /locus_tag="PK610_00240"
     CDS             complement(23723..24049)
                     /locus_tag="PK610_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRJ34379.1"
                     /translation="MDARRDVFQAIADPTRRAIVLLLAVGSLTPNAIAEHFNSSRQAV
                     SKHLKVLAECELVRAEQQGREMHYHLNKNKLKEIEKWLEQFKQLLEKRFDQLDNVLEQ
                     LKTKRK"
     gene            complement(24187..24741)
                     /locus_tag="PK610_00245"
     CDS             complement(24187..24741)
                     /locus_tag="PK610_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34380.1"
                     /translation="MNYNYSFGKDDKFMFSGLNAFVKYSSHELYFGFNTNNALLPDFS
                     KTSINVTGFDCGIKYHFASLNRISHFFSELNIQRHRYIKSICTSPRSINFPSTGEEHC
                     HVSKCTSTNIHLAAGYETFFTKRISLPLSFGGGISRINTTSLNNPAFPATSGISWIFS
                     PFVKVALRVYVGKNEVSSKADNKQ"
     gene            complement(24831..>26567)
                     /locus_tag="PK610_00250"
     CDS             complement(24831..>26567)
                     /locus_tag="PK610_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HRJ34381.1"
                     /translation="YSPYKTLNEDQFNSVLEILNGLVGKKSNQIFVRGSAGTGKTILA
                     TYLIKLLNTNFDDNLDDINENELTELNYVRKFRSKYPNPKIGLVVAMSSLRTTLENVF
                     KNMPGLKATMVISPADTFKLKEKYDLLIVDESHRLRQYKNISWRGAFKANNQKLGLGD
                     EGNELDWILANSKNQIFFYDSAQSVKPSDISEKHFKKLLDSDKSVKLELKSQMRVKGE
                     DNNNYISFVDDLLHVKPKEQVFSPRGYDLLFFDSFEQMYQELKKKEEQFGLSRMVAGY
                     AWEWLSDPKRKPKPKANVTDFDFDGLKFKWNSTHIDWVNSPNAFNEIGCIHTTQGYDL
                     NFTGVIFGWEIDYNPQTNKIEIDATKYFDKYGRIGADETELKNYIINIYKTIMFRGVR
                     GTYIYACNPNMKEYLKRHIETFVPSIGIRFLDYDKVKPYVNSVPFLDIKAAAGGFSGF
                     QNYKELKWVELPENIAARKGYFVCQVVGDSMNKEIPNGSLCLFKQDEGGSRNGKIVLV
                     ECSDIQDADTGASFTVKEYSSVKSKDKDGWAHEKIVLKPRSTFSTYKDIVLTMEDMSS
                     YKVIGLFDRVIL"
BASE COUNT         7801 a         5882 c         5731 g         7153 t
ORIGIN      
        1 agtaattttt tcatgtctga tacagatttc agacagcaaa atctaaaaaa aaaaatgaat
       61 tcgcaaaata ttaggcgaac ataatataca aaaatgaggc aattattaca tttctaacct
      121 acaaatactc cttcctaaac ggaaaggtgt acttgaaatg gctttgcttc cagccttcgc
      181 ggtcgtagaa gcggtgggca tcggttcgtt ttttgttggt gcacaattcg atcagatcgc
      241 aatcgcgctc acgggcgagt tgggtcacgt gatcgagcag cattttcccg tatcctttcc
      301 cgcggcaaac atcgctgatg cagatctcct gtacctcagc caccggtgca aaatgatgca
      361 gcaggtattg aatgtgcaga ctcacaaaac cgacgattat tccgttttct tctcccacga
      421 aataataaat atcctgcatg gcgcaattgc gcaaatacac cggcgcgaat tcttcgaagg
      481 gaatgctgca ttcttcgagc tgttcaatga gttgatgaac ggcggggaga tcggtgtggg
      541 tggcgggaag gatgtggaac atgattgggg atttgttttt ttaccactaa ggcacttaga
      601 acacgaaggt acactaagaa agaaagatac tcagtatatt taagaatgaa acgagatgag
      661 catttgctga atgtacgacc gaggttggat gtggaggatg atttgtctct gcccgaggag
      721 gtgtttcaga acgaggtgat ccgtccggtg atcaagtttc agtcggaggc tctgtttgcg
      781 atgatgaacg aatacatccg tcgcctatcg cccgatttca gaacctacga tcttgtgcgc
      841 aaacgcaaat tcatttcttc agccatacaa aaagatcaca ccttgcatca tcagctcatc
      901 ggaatggtgg tggcctgttt tacgctggaa gaattgcagc attttctgca acaacgcagt
      961 gaacattcca aacgcatttc gcaaattctt acggaacgtt tttgttcgca agctgagaag
     1021 ttggggtagt gaacggtgat cagtgaacag aaaccgatat tctgtcttag tgcctcttag
     1081 tgcccttgtg ccttagtggt gaatacagat ttaaaatatt cgtaaaccac caccaccgta
     1141 ccgagcatgc ccaggttgta aatggtgtcc tctacaggaa tggtaccgat gcggatgttg
     1201 aaaatctcct ctgaattgta ccaaacaatc ggctccgcaa tccaggaacc ggtgagcatt
     1261 ccgttaacaa gtaaaaaggg aatcagcgta acgagataag ccagaaaaaa acgactcatg
     1321 taaggcgatt taacaacgta cagcattagg agcagcaaca ctcctgcata gggaaaagca
     1381 gtggcgggat accattttac gtaattgacc agacctgttg caatcaaaac aatagcaaga
     1441 attgtcagca agggtttgta cacttttgcc aggagatcgg tcttgacata agcattgagg
     1501 cattcataaa tgaagataca ggcgtacgga atgcaaatga aaaacaacca ttcctcgagc
     1561 ggcaaattga taatggtaat acccagcagg taatcctcat tgaatcccca gacctctttt
     1621 gcagttttta ccacatccca gggaatgaag acaagcatca taatggcaat ggcaggaaaa
     1681 agggctttcc agtttttgta gaaggctact tttttatcga agctcagcac cagcggacca
     1741 agcagcgaaa aaagatcgat gaacaaatac agcgatttca cgccttttgt ttttggcgca
     1801 tgctctcgcg ggcttcgcgg aaatacttca gcggaacaat cagcattccg aaacattctc
     1861 cgtcttcctt gccgaggtgt ttgtggtgca ccttgtgcgc tttgcgaata gcgcggaggt
     1921 aaacattgtc ggaattttta aaccaagaca tgcgtcggtg aatgaacact tcgtgcacca
     1981 gaaaatacgc aaaaccatac accgcaattc cggcgcctat agatacagaa atataattcc
     2041 catacatcat tcccaacatg atgcacaacc agcttggcac agcgaaaatc agaaagaaaa
     2101 tatcgttgcg ttcaaaaaac ccaaacaatt tgggaatggg ttggtgatga tcctcgtgga
     2161 aataccacat gaatccgtgc atgatgaatt tgtgcatgaa ccaggccatg aattccatca
     2221 cacaaaaagt tgccaatacc gttagggcgt aaatcattgt ttaaaaatac gttgttttat
     2281 cgcgttccca accaccagaa gaaaacagcc tgaagtacaa acaaacaaat ggcggcaggg
     2341 ttcatttttg gacgaaaaac cttaaccatg agcacctgga agatgaatcc aaagataaac
     2401 catgagaggt aatttctgaa aggaatggta tcgtctttcc attgccagta atccagcttc
     2461 atcgcaaccg gctcgatcag aaaatccatt cctgtcatga gcagcgcacc aaaaaatgca
     2521 atcaacaacc aatgcgaaaa taaaacagat gcgacttgtt gcgtagcata aacgagcatc
     2581 agccagttga gtccgatcac aatcggcacc tcatacattt tccaaccgag gttagcgcca
     2641 taagcatatt ctccgaacaa agccccggtc atgaccccaa ccatctccac cacaaagctg
     2701 aggacgaaaa tcgaaatcat caccagtagt tcacttccgc ttaatttacg cagcggaaaa
     2761 aaaacaaaaa caccactcat aacgagattc acccaggtaa gcgatgcaaa ttccaattga
     2821 ttctgtgttg caaaacccca gaaacccaca gcgtaaaaaa tcactaacac agcaattgca
     2881 atccacaagc gcaggggaga aaaactatcg ttcataagct ttgatcttca agaattaaat
     2941 ctgcagcaat tttcgcagaa cgcaaacaca atggaattcc accaccggga tgcacactac
     3001 cgccgcaaaa atacagattc ggaatgcgac gcgaaaaatt agggtgacgt aaaaacgcag
     3061 cgtaacggtt attggaagca gaaccataaa gcgaaccctg gtaggaaaaa gtatcccgct
     3121 caatggtacg cggttcccaa cgatcttcct gctcgatgag agaagcaaca tctgtattaa
     3181 gcatacgcga aagtttggca atggtattcg cacgaatttg cggaatcagc gcgtcccaat
     3241 cctgaccatt attcccggga acattcacca gaatgaacca gttttctttt ccatccggcg
     3301 catccgtttt acaatattta gatgagatgt gaatgtaaac ggtaggatcg tcggttacac
     3361 tgttctcttt aaacagcatg cggaattcct tttcgtaatc ggcgctgaaa aaaatattgt
     3421 gcaatcccaa ctccggaaat tggcgactga ttccccaata gaaaatcagt gcagagctgg
     3481 agcgttcctg ctgcaatgat ttttcaggtg ctttttcatt gggaagcaaa cgacggtaag
     3541 tcggaaccac atccatattg ctgatcacca catcaccggt aatttctttt ccattcacac
     3601 gaattccgct tgcttttttg ttttgagtga tgatcgtttc gaccggggca ttaaaatgaa
     3661 atttcactcc cagttccaat gcaagtttgt aaatgacatt cggaatatcg atcatccccc
     3721 ccttgggaaa aaaggttccg aggttgtgtt ccagatgcgg aatcatagaa agaattcccg
     3781 gcgcctgata aggatcgctt ccgttgtaag tggcaaaacg atcgaagagc tgtaccaggt
     3841 ggggcgattt catgcgcttt cggtgcacct gatgcatggt acggaaaaga tgtaatcgcc
     3901 aggcattcat cactccgcgc aaaacatctt ttcgcaaata attttttgca cgatgcagag
     3961 aacgttcgag aaaaacacct tcggtaagat cgtacatgga tttgcactcg gcaaaatagc
     4021 ggagtatatc ctcagcagga acagaaaatg ttttggcaca ggccgctgcg aaatcttccg
     4081 gctgcgaacc cgctgcgagg cgaaatccgt cttcccagaa atacctgcaa ctttccggta
     4141 tgcgggtgta ggaaaaatgc tgcgcaggat cttttccggc cagcaaaaac aattcatcga
     4201 ccagcgcagg aagcgtaaac aatgaaggac ccgtatcaaa acgaaatccg cccaggtttt
     4261 tctccgataa ttttcctccg ggatgatccg atgcttcaaa cacctcaaca gcataaccct
     4321 ttaccgcaag acgaaccgct gttgcgagtc cacctatacc ggaaccgatg atgagggctt
     4381 ttttcatgtg ttcaggttaa caagcaatat ccttacttgt ttactttttc cgtttgagtg
     4441 atggaaggat gtagcacaat tgcacgtatt cttcgaggaa aaagaatttc tgactggctc
     4501 cgtgagctcg atcggaatag gtgaggatat tgggcaaatt gatccaaccc ggttttacag
     4561 ttgcacgaag gaaaaaatgt ttccccatat ccaatcgcaa tcctatgtgt gcgctcaatc
     4621 cgtaacctgc agtatgaaac aaattagctc cgggatttcc aaaaacggta atatcactgc
     4681 gtggataaag aacaccaatg cctcctcctg cttccatgct tagtttcaat ttgtatttac
     4741 gtgagctcca aagcgaatcc ataaaatcaa tttctgctcc gaggaggttc agtccgttag
     4801 tatgttcgaa atacaaaaag tcaggtgaca acatcattac actatcgttg tactgtccgg
     4861 caaattgcga agatgcagct tcagtgatat acccctccat caatacaggc tgccagttgc
     4921 gcatgacgta tttcatgtgg tcgtcaagaa gtgatatgct gatcttatcg gtgaggtaat
     4981 acccgatttt gaaattgtac tgaggccggg ccaggtgtac gggattggca tatactctca
     5041 gattaaaagc ctccggacga tcactggcca ggacattatc caggacaaaa tcgtatccgg
     5101 gtccccataa gtggatatca ctggaagaat actgggcccg attatagccc cagccgaaga
     5161 aaaatcgtcc ctttcgcacg atttccgtat tctgggattg gcctgaaaag gatgaaaaaa
     5221 gaaggaaaag tccgagaaaa accgataatc tgttcatgtt cacaaaggta gctattgact
     5281 ttgattaaaa aatgacttaa ctttcccttt ttagattagg atatacatca tctatcgcta
     5341 tgcctaccgg aacagttaag ttttacaacc gcaagaacaa atttggtttc atcaaggttg
     5401 atgacaccgg cgcggagatt tatgttcgcc agagcgggat tgtcgacccc attaatgagg
     5461 gtgaccgtgt ggagtttgaa atggaagaac acccaaaagg acccatcgcc gtgaaagtga
     5521 agaagctggc tgctgaataa gcgtctgttt ttccgtctcc tgcaacgaag cgattattcg
     5581 gtttcgtgac tcccttcttt tttctgattt ttgcggcgtg aaagccgacc gccatattct
     5641 tgttctatgc atgcaccaca tcgatcgtgc tcccaatcag cgctttcgca ttgagcagta
     5701 tgtgccctat ctccgggaaa acggtgtgga gcttaccttt tcgcctttat taagcaaaga
     5761 agatgatgcg gtattctacc gtccgggcaa tcttttcaaa aaaatacaga tcgttcttcg
     5821 ctccatacgc caacggcgca gggatttgca gcgtgcgtcg caatttgatg cggtactgat
     5881 cattcgcgaa gcactcttta tcggtccgcc ccgaatagaa agaaaacttc gaaaaaaagg
     5941 gctgactgtc atttacgatt tcgacgatgc catctggaag ctggatgtat cgcgggccaa
     6001 ccgctggttt ggatttttga agttcccggg aaaaacggca gctattattc gtcttgccga
     6061 taaggtcatt gccggcaacg aataccttgc agattatgca aaagctttta acccgaatgt
     6121 gcacatcatt ccaacaactg tggaaacccg tacattcctc cccggaaaaa aagacgcttc
     6181 ttctcctgtt gtcattggct ggagcgggag tcttaccacc atcaagcatt ttcggattgc
     6241 agtgccggta ctcaaaaaat tacgcgaaca ctatggcgat cggattcgtt ttaaagtgat
     6301 tggtcacgca ggattctcag aggaagaatt gggtatccgg ggaacagcct ggtcatcgga
     6361 aagagaattg gaagaattac aggagatcga tatcggcatc atgccattgc ccgatgatga
     6421 atggtcgaaa ggcaagtgcg gactcaaagg attggtttac atgtcgatcg gcattcccag
     6481 tgtgatgtcg cctgttggag taaacaccca aatcattcgt cacggagaaa acggatttct
     6541 cgcaggcact gaagcagaat ggtttgagat cttatccctc ctgatcgaaa gccccgaact
     6601 gcgcaaacaa atcggctcct caggccgcca aaccgttgta gaaaactact ccgtacttgc
     6661 caaccgggaa aattatctga acatacttgg taaatcctcc taaaatacat cctttgtcct
     6721 ccgaagcttc agcgtagggg gacatctgta aaccccaatt tctgttacat ttgcgaaaac
     6781 ccccttcatc atggaaacaa ctactgcact taaaaacacc gcactcaccg ataaacatat
     6841 tgcgcttgga gccaagatgg ttccttttgc gggatacaat atgccggtat catacagcgg
     6901 attgaatgat gaacatgcaa ccgtgcgcga agcagtagga atgtttgatg tgagtcacat
     6961 gggcgaattt attctccgtg gaccaaatgc tctcgatctt attcaacgtg taacatcaaa
     7021 cgatgcatcc gtgttggtcg acggaaaagc gcaatattcc tgtttaccca atgataaagg
     7081 cggaattgtg gatgatttga tcgtttacaa aatagccgat gagcactaca tgctggtggt
     7141 gaatgcttcc aacatggaaa aagactggaa ctggatcagc aaacacaaca caaagggtgt
     7201 cgatatgaaa aacatcagcg acgacacttc attactggca gttcagggac caaaagcgat
     7261 ggcgacattg caaaagctaa ccgacgttaa gctctctgaa attccctatt atgcgtttcg
     7321 tattggcact ttcaatggtg taaacgaagt tgtcatttcc aataccggtt acaccggagc
     7381 aggcggattt gaactgtatt ttcccaatgc acatgcagag aaaatctggg atgctattct
     7441 tgaggcagga aaagaatttg gtattaaacc gataggatta ggtgcacgcg acacacttcg
     7501 tttggaaatg ggattctgtt tgtatggaaa cgacattgac gataccacat caccattgga
     7561 agcaggattg ggatggatca ccaaattcac caaggaattt accaatagta aagcgatagc
     7621 cgagcaaaaa gaaaaagggg taagccgcaa gttggtcgga ttcgaaatga tcgacagagg
     7681 tattccgcgt cacgattacg agattgcaga tgcagccgga aatatcattg gaaaagtaac
     7741 atcaggaaca caggccccca ctttgcaaaa aggaatcgga atgggttatg tgagcacatc
     7801 atttgcgagt cccgattcag aaatttttgt gaaagtgcgt gataaagcac tcaaagcaaa
     7861 agtggtgaaa cttccttttc tcaaaaagta attttattcc gctttagcaa tgagtcaagc
     7921 gacagtagac gcctgttttt ctccgtatct ctatccggta taccggaacg acgataacat
     7981 tgtggtgatc atcgatattt tgcgggcgac atctgccatt tgcaccgcat ttgagcacgg
     8041 agtagaaaaa atcattcctg ttgcaaccgt agatgaagcg cgcgaataca aagcaaaagg
     8101 atttcttgca ggtgcagaac gcgatgctgt tcccgtagaa ggatttgatt tcggaaattc
     8161 tccattcagt tacatgggag aacacatcaa gggacaaacc attgttctta ccaccaccaa
     8221 tggcacacaa gccatcaatg cggcaaagga tgcacacaaa gtcataattg gtgcatttac
     8281 caatatcacc gctgtttgca attacctgaa atcgcaggac cgcaatgtga tgttactttg
     8341 ttcgggttgg aaaaataaat tcaatctcga agacgcattg tttgccggtg cggtaaccga
     8401 agaactcagc aaacatccgg agctgttcaa gctacaggac gccgcacttg cattgaaata
     8461 cctctatcag caggcgatgg aagatccgca taaattcctg cacaacgcat cgcacaaaga
     8521 gcgtttggcc aaactgggat taaaagaaga tatcaagtat tgtttgcaga acgatctcac
     8581 caaagaaatt ccggtgctgg aaaacggagc gcttgtacgg ctcaggtatt aagttttttc
     8641 tttcagagag gagggatttg caagtgcggg aatgtacaca ggaaggctcc tctcattcgc
     8701 aacttcaatc aatcctcctc ctcaaactcc aattccgcat ctttctccca gatctccatt
     8761 tcgcaaggtt tgcaattgaa ttttagtttg tattcgttgg taccgtgctt caaggtgagc
     8821 ggttctgtca acttctctcc catggtttca cgctgaaaac gcggacattt tccgagttcg
     8881 tatttcacac aatatttcgt taccatcacc cgcgattttc cgggatccca ctgaagctcg
     8941 aatgcttttt caatttcagt tacaccgtga cgcttgtaaa atgcacgcgc taacttattg
     9001 gaaacattat aggtaaaatc aagtttttct accggataag gatgatctgt ttttacatgt
     9061 tggtgctctt cgcgtacata ttctttcagc cgcacatcca tcaattgatc gagcacatcg
     9121 cgacgcattt cattcacttt tgaaatcggt aaaaaccaat tcgaagaaaa ttcaatcgtt
     9181 atttcgtcca ccacaaaagg tgtattcccg gccttcgcca gattcttttt gatattgggt
     9241 tctgccgatt ccgcactctt tgcaagttct ttgttaagca ccgcttctac aatcgctttg
     9301 tgaccatctt catcgcatgc ctccagtaaa aatccctgcg ttgtatcttt cagtgttaac
     9361 tgtactccta tttttcggat aacactgtct tcgcgttcaa cgaggcggtt aaattctgca
     9421 tccgaatttc tgtagatgac tgtcccggga gcgagcggtt taaatacatt gggaacaaca
     9481 acatcgttga tgatgatgtt gatctgtgca ccgtctgctt ctccgttttc gttgatgaaa
     9541 tacaaaccat caccgttgtt cagcagatcg gaattttcga ttacatatcc attcgctttg
     9601 gtctcgatca gtttgccgat gagttgtccc tgcgacttcg ggctctccca ggagcctata
     9661 cgctctgtgc gtttgtttac gaaataatcg gtgtatccgc ggttaaaact gcgatcgagt
     9721 ttagcttcaa aattgtagaa tgttcttccc gatgatgctt tctcaaaatg aggattgttc
     9781 tccagaaatg catccagttt tttacgcaga taagaggtgt tgttttttac atacaccata
     9841 tccttgagac gaccttcaat tttaaaagaa gaaattcccg cttcaatcaa ttgcggtaac
     9901 tgatcagaaa gatcaagatc cttaatggaa agcaaatgac tatttgcgat cagtgttgtt
     9961 ccattaccat caatcagctg ataaggcaaa cggcaattct gcgcacacga acctctgttg
    10021 gcgctgcgtt ctcctcctgc gatactcatg tagcaattac cgctaaagga aacacaaagc
    10081 gcacccgaaa caaaaaactc cagttcaaca tccgtagctt catgaatttc gcggatctga
    10141 tccagattca attcacgcgc aagcaccaca cgctgtattc ctgcctcggc aagaaatttg
    10201 acatgtttag ggtcgcggtt gttagcctgt gtgctggcat gaattacaat tggaggaaga
    10261 tccatttcca ggatagccat atcctgcaca ataagcgcat ctacaccaat agcgtaaagt
    10321 ttatgaataa gcttacgaca ggcttccaat tcgttgtcgt agagaatggt attgataacc
    10381 acaaaaactt ttgccttaaa caaatgcgca tactccacca tttcggcaat atcatctacc
    10441 gaattcgttg cattcgatcg ggcaccaaaa gcttcggcac caatatacac cgcatctgca
    10501 ccggcattga tagctgccat cccctgaata aggtttttgg ccggagcgag aatttctatt
    10561 tttcgtttca taaagccatg caaaagtacg aaaatgaagg gaatccacta caattccacc
    10621 tgtgcttcag tcctccttcg ttcactgcta tggataggta aatgaatccc aacacttatc
    10681 aaagaggctt gaagagagtc tgtgacgcag aaaaacagct tcgaattgac ccccttcaga
    10741 aattgcagtt tgttaacata tactgggttg tgcttttgta tatttacatc atgcgtcgcc
    10801 ctattcttct atttgttctt gtgctcttgc tgatgccggt tattggtttc aggatgatgg
    10861 gccattcctc atggggcttc tatgcgcata aacgaatcaa ccgcatggcc gtatttacat
    10921 tacctccggg tgtgttcgga ttctacaaag acaatatcga atttgttacc gatcatgctg
    10981 tggatcccga taagcgcagg tacgccgtaa ccggtgaagc gcaacgccat tacatcgata
    11041 tcgatcatta caaaatagaa gaagaagatg tgtttgttac cgttccgaaa aaatggaagg
    11101 acgctattaa aaaatacacg gaagacactc tgcaggccta cggaattgtt ccctggcata
    11161 ttgaggtgat gatgtttcgt ctgaccaaag cctttcagga agaaaattac gatcagattc
    11221 taaaactcag cgcagatctt ggtcactatg taggcgatgc gcatgtcccc ttgcatacta
    11281 ccgaaaacta caacggacaa cttacaggtc agaaaggcat tcacggattt tgggaatcgc
    11341 gtataccaga attgtttgcg gaggaatatg attattttgt tggtaaagcc tattacatcg
    11401 aagagcccct cgagtttgcc tggcaggctg tgaaagaaag tcaccacgct gttgattccg
    11461 ttttgaattt tgaaaaaata ctcagcaaac aatttccgga ggaccgcaag tacagttatg
    11521 aaacacgcgg taacgtaacg gtaaaagtgt attctcagga attcaccaaa gcctacagcg
    11581 atatgatggc aggaatggta gaacgaagaa tgcgcaagac aatcattgct gttggcagtt
    11641 tgtggtacac cgcatgggta aatgccggta aacccgactt aaacaaacac aagaacaaac
    11701 aaatctcgcc tgaaatgcag caggaattac aagcagaaga taaattgcat caggaaggaa
    11761 aaatcaaagg aagaggttgc gatcacaatt aaatccgaag tgattaccag ttaccaatta
    11821 cccagttacc aattacccag ttaccaatta ctgttgatcc ggatgtaaaa ttttcggatg
    11881 aggtcagcgt attgtttcgg gatgcgtgac gctttttgaa tcagatctcc tttttagagg
    11941 cagtctttcc cataagcatt cgggtgagtg tgtagaaggt cagcaacaaa gccacaaaca
    12001 aagcgctgcg tcctataaaa tcgccatggc gggtaaacca ggtttgttcc gtattggcaa
    12061 aaaccactcc gcgcaacaca gcgggttccc accatttggt ttgctgcact acatctcccc
    12121 gctgattgat gaaacaggaa attccggtat tggcacaacg cgcaatgctt cttcggtttt
    12181 cgatggcacg caatcgcgaa tacgacatgt gctgtttgta ccccggagta tcttcccacc
    12241 agccgtcgtt ggtaattaca aaaatcaaat cgccacccag tcgcacaaat tctgcgatgt
    12301 gttcaccata aatcgattcg tagcaaatgg caggaacaac aacatgacgc ggatcttttt
    12361 gtgcggtaaa aacttttggt gccgtttcag atcccatcga gccactgctt ccccccatgt
    12421 cgagggccaa cttttccaga aagggaaacc atccggaaaa tggaactttt tcgacaccga
    12481 gcacaagttt cattttgtgg tacacatgca cggctgcatt gttctctatt tgcagggaag
    12541 cattaaaatg atcgtacaca tatcccggtt ctgaagtagg ttttgcagtt ggtgatttgc
    12601 gacttccttc acgaaatact gtgtagctcg acattcccgt tacaattttc aactgcggat
    12661 aatcactgca cagattcctg aacgctttta cagctgtgtt gctgtcgata gcatcttcat
    12721 ttaccgaaaa aggaatttgt gtctccggtc cgagcaggta ttccacgttt tcatccagac
    12781 ttttcctggc gagatcagcg taaatatcga actgtgcctt cattgtcaat ccaccgaatt
    12841 tttcgttgta gggatcgata ttcggctgaa caattacaat atccacaggc tcccctttat
    12901 cggaacagga agaatacatc ttcaggctga tgagtattgg cgtaaacagc agcagcgcaa
    12961 taaaaatgaa tcgtgatagc tgttctttca tgctttgttt ctgcaaccag aacacatcac
    13021 gaacaatccg gaaaaaaaga aaattcaaca ccaaaatcca aagcgtgcca cccgttacac
    13081 cggtaatctc gtaccattgc acaacagaag gaaccatcgc aaaaacatta ccgaacgaca
    13141 accagggcca tgacaattcc caactatagt gcaggtattc gaaagccagc caattgagaa
    13201 aaaaagcgat aattccttcg cgcgaaccaa cacggcgacg ggtaacatgg aacagccaaa
    13261 aaggaatggc cattaacaaa gcattgaaaa aaaacgccat caccgcacca ccggccgaag
    13321 cataccagat ccaccaggta gttgcacaat tgaataccag aaaaaaccag taagcgtgca
    13381 gaaagacttt acgcggacga taccctttgc gaaaaacatg ttcctccagc aaaagcatag
    13441 gaaccaaggc aataaaaacc acaggccaga gggaaccaat gaacggccag gccagtgcca
    13501 tcaataaagc agtgagtgta gaatatacga tgagaggaag cttactcatt ggttcaaaga
    13561 tagaaattca atttgctccc gcacaagacc gctaaagaat gtggagaaca tcccttaatc
    13621 cgcccgattt tggtttagga tatgtccgat attccgctat attcgctttc atgaaatcag
    13681 tatttttctc cttagtggca gcactactcc ttctgcccat ttcccttcag gcacaaactg
    13741 cgaaaaaaaa gatcgaccac acagtgttcg acagctggaa actggttcgc gcagcaaatt
    13801 acagttccga cgggaaagtc atcacttacg aaattcagcc acagtcaggc gacggttacc
    13861 tttacgttta tcgcatccag gaaggcaaac tcgattcaat tgcacgcggt tatgaagcac
    13921 gtttcattga cggtagcaat ctggtgctgt gtaaagtaaa acccgcattt gcagctaccc
    13981 gtaaagcaaa actcgccaag aaaaaagaag aggatctgcc gaaagattcc gttgcgcttt
    14041 atcatgtaga gcaaggcaaa gtgattgcag gaacggtaat ggtaaaatcc atttccggaa
    14101 aagaagaaag caaatggtac gcagtgcacc acagcaaaat gccggagcct gctgctactg
    14161 gtaaaaagaa gaaaaagaaa aaacctgcac ctgaaattaa atcggaagga acctggctgg
    14221 aactggtcaa caccacacac gtctggaaaa aagccgatcc caaagcgaaa aaaccaaaaa
    14281 atgccgcaat ttctcaatac cgttttcagc atgtaaccga atacaccctc tctaaaaaag
    14341 gggagcggtt tgcatttgta caacagcgta aagtcggaaa aaccgattcg gcctacatca
    14401 ctgttttcga tacacaaaat ggaatcccca cgcgcattta ttctgttgcc ggtttttgta
    14461 aagcactcag cttcgatgaa aagggagctc aacttgcctt cctctcgagc acggatactt
    14521 caaaaaacaa agtgtacgat ttatggtact ggaaaaacgg tcacaccgca ccgagtctgc
    14581 ttgtggatgc tgctaatacc gccatgccca aagactggtg cgtaagcgaa aacggaacac
    14641 ttcgtttttc ggagaatgga tcacgtctct atttcggaac agcagaaaaa ccacagaaag
    14701 aaataaagga cacacttact gaggatgaga aatacaaact ggatgtatgg agctggaccg
    14761 acacccgcat tcaacccgag caactcaaac aactcgagcg cgataaaaag aaaaactacc
    14821 tggctgttta tcacctcagt acttcaaaaa tggtgcagct ggccgataaa gaggtggaat
    14881 tcgtgcgcac caatcaggaa ggaaacggaa cacttgcact ggggatcacc gaagcgcctt
    14941 atgaacgcag caagacctgg gaatatccta ttgccagcga ttattattcg atcgacatca
    15001 acaccggcga aaaaaagctg atcctgaaag accagaaatt ctattgcgat ctttccaatg
    15061 caggaaatta tcttttgtat tacaaacgca aagacgataa ttggtatgca cttgaaattg
    15121 caagcggaaa aaccattaac ctgaccgaaa aaattacgga agactttttc gacgatgaca
    15181 atgggaatcc ttccgaagaa gatgcgcagg gaattgcagg ctggtcagaa aacgacggtg
    15241 tggtttacct caatggaaaa tacgacatct ggaaagttga tccgcgcgat ccatccaaac
    15301 ccgtttgtct gacaaaaaat ttcggacgcg aaaccaaaac tgtttttcgt tataccgaac
    15361 tcaaccctga agcaaaattc atcagtgaaa acgacatgct gttgcacagt ttcaatgagc
    15421 gcacaaaaaa agcaggatac tacagagtta atctcgacgg caaaacaccg gcagttctgt
    15481 tggaatcgga tcataagtat ccctatatcc ttaaatccaa aaacagcgac aaaaccgtat
    15541 ttgcacgaat gaattttctg gaatgtcctg atttatggat cagcgatatg aatcttcaga
    15601 acattcgtcg catcagcaat tccaacccgc aacaaagcga atacaactgg ggaacagttc
    15661 gattgaccaa ttggaaaaca cctaaaggaa gacaactcga cggactcctc tatttgccgg
    15721 aagattttga cagcacaaaa aaatatccgc tgttggttta tttctacgaa aagtatgccg
    15781 acgatattca caattattat tcacctcgtc cttcggcatc catcatcaat ccgagcgaat
    15841 attgcagcaa tggttatgtt gtttttattc ctgatattct gtacaacgaa ggcacacccg
    15901 gagaagacgc ctacgactgc atcatcagcg gaacgaatca cattgtaagc aaaggattca
    15961 ttaacaaaga acgcatgggc ttgcagggac aaagctgggg cggataccaa acagcttatc
    16021 tcgttacgca aaccaatatg taccgcgcag caatggcagg agcgcctgtg agcaacatga
    16081 cgagtgcata cggaggtatt cgatggggaa caggactgag cagaactttt cagtatgaaa
    16141 aaggacaaag ccgattaggc gttacgctat gggaagaccg cgagcgctac atcaaaaatt
    16201 cgccattgtt ttttgcagac aaagtgaata ctcccttact cattatgcac aacgaccgcg
    16261 acggtgcagt gccctggtat cagggaattg aatacttcaa cgcattacgt cgtcttgaca
    16321 aacccgtttg gatgctcaac tacaacgatg atgatcataa ccttacacgc agagccaacc
    16381 agaaagacct gagtatacgt atgcgtcagt ttttcgatca ttacctgctt gatgcaccca
    16441 tgcctgtttg gatgaaagac ggattacctg caattgacaa agggaagaga acggggtatg
    16501 agttggagaa gtaattttta aatcctcatt tagattaccg atcctgttca ttagaaattc
    16561 agcccgttaa ttttgttcta tgaaacatgt ttttactaca tgtttttcag aggaaattga
    16621 aaagccggaa accataataa catctttctg atccagcagg atatcgttat ccaaaacgtc
    16681 tttttatcgg tttaggcatt ttgtttgatt cattcacaaa acaaattatg ccattcacaa
    16741 agaaatcctt caactgaagg acaatggcag gggtttcgcc tattttagcc tatattctct
    16801 gaaaagaata aacaggtata aaaaataaac tatgaaacgt ctttttacta aagtggtttt
    16861 tgcattgttg atgttgcttc ttaacaattc tatgcatgca cagaatatca atttcagcgg
    16921 aaatatcagc accaacacga tttggtcgag cgctgccatt gatacggtaa aaattacagg
    16981 caacgttacc atcgatagtc tggtaacact aaccatcgaa cccggaattg tggtcgattt
    17041 taccggatat tattacttaa atgtaaaagg aaccttgcgc gcagtgggaa cagtaaccga
    17101 ttccatccgg tttaccacac gcgatacact tggtttctcc ctgaacaatc acacaggttg
    17161 ggcaggaata cgaattcatg cgagaaacaa caccaacgac acaagccgat tttcgtattg
    17221 cgttgttgaa tttggaaaag cgaacggcac atccgggctt cccaatgcgt cacatggagg
    17281 aggattgtat ttttatcaga gctggaaaat tattctgaac aactcttcaa taagaaacaa
    17341 ttatgcacaa accgcaggtg gaggattata tgagtattac tcctacgtaa atacaagcaa
    17401 tgcgcaaacc attaaaaact gcgtttttaa aaacaataaa agtgcaaaca ccggaggcgc
    17461 gatttacacc cagcgtggtt tagaattagc aatagaaaac tgcaccttta tccggaacac
    17521 tgcagccagt tcaggtggag caatctttgt tgaaaacact tccacttcac gtgctaaaat
    17581 cggcaattgc agattcagct acaacaatgc tattggcggt ggagcagtaa gcgtgtatgg
    17641 aaccttgtcc ggtggttata ttaatgattg cacactcaat aacaataccg cacaaaacgg
    17701 cggcgccatt caggtgagtc aggcgctttg cgatatcaag aattgtgtca ttgtaaacaa
    17761 cacggcaacg tccaacggtg gaggaatcta tttcaataat gtcttctcca atttctcagg
    17821 caccaatgcc attatcaaca acaccattgc ccacaataca gccaatcttg gtggtggtgc
    17881 gtatatattc gataattctt ccggagcaga tttgtacatc aacaaaacta ttttttacaa
    17941 caatgtgtct tcaaacgccg gcgcattcaa taacgacgtt tatctggaac agtttttagg
    18001 aagcaaccaa acgcgctttt acggttgtag tttcaccgca acgctggcaa acaccaccgg
    18061 aaatccgaat aatattggtt cgctcctcaa tacaaacccg caatttgcaa accccagcgc
    18121 aggaattggt gcggccttcg aaggatttac aggaagaaac tggacattaa atttttgtac
    18181 ttctccatgt atcgacggta ccaacgacaa tgtggcgtgt gcctgctttc ctgcaacgga
    18241 tagaaatggt aatccgcgta aaacaaacaa ttttgttgat ataggcgcct atgaaagtga
    18301 tggatttatt acgcaacctc tctctgtttc ggcttgcgtt ggtgacgcaa ttaatttctc
    18361 gtacgattac ttcttcaata cgggatcaac acctaacagt tattgggagt attctaccaa
    18421 tggcggagct aattacactt caatagcttc cgcatcgaac acctatgcct atacaacagc
    18481 tgcattgtta agcgatcata atgttttagt tagagtgcgt cacgattatg gatgccgtga
    18541 agtctattca acggtggcta ccatttccat ttttacacca ttaaacatca cagcaaatac
    18601 aaccgcaagc agtgtttgtc tgggcacacc ggttacgttg agtggatcgg gtgctgtgtc
    18661 gtatagctgg aataacggtg taacaaacgg aattgcattt acacctgcat caacaaacac
    18721 ctatactgtg gttggaacag atggcaatgg ctgcgcaacc aatcctgcta ctgttacggt
    18781 aaccgtcaac aacaatccaa cagtaagtat taccgcatca gaaacctcca tttgcaatgg
    18841 cgatgacgta acactaagcg ggaacggagc gctgtcgtac ctttggaaca atggcgttgt
    18901 aaatggtatt tcatttattc ctgcttccac cactacctat accgtaagtg gaacagatgc
    18961 gaatagttgt gtgggtactg caacagttac agtaacggtt aatcaaccta ccatttctcc
    19021 aatagcaagt tcaaccggag tttgcgaagg aaatgctgtt acactttcag gaagtggtgc
    19081 ggtaagttat acctggtcgc acggcgtttt ggatggtgtt tcttttgttc ctgctgcaac
    19141 tgcaacctac actgttaccg gtgaagattc cgacggatgc acgaacacgg caaacatcac
    19201 cgtaacggtt cattccttgc cggtaatcag catcaatgca aatcctgcaa cagtttgcgc
    19261 aggtacagct acaacactta ccgcaagtgg tggtgtaagc tatcaatgga tgccgggaag
    19321 tttatccgga acaagtgtta gtgtttcgcc ttcggtgcag actacttata cggttatcgg
    19381 aaccgatgca aacaattgtt caagtgatga aaccattgaa attatcgttc ttgatttacc
    19441 gaatgcagat attggtcctg caagtttaac agtgtgcgat ggcgatcaac taaatttttc
    19501 attgagcagt actgcaggtg tttcaaccta ttctattgta actgcattcc ataattcggt
    19561 tgatgctcca ttgtcgttcg tttacgacgg aaataatcct ccctcactcg ttcagtgtaa
    19621 ttctaccgga acaaacggat gtactgcttc tcaggtggtt cagctgatca ataattctat
    19681 tcttaatctt tcgcagaaca ttccaaccgg aactgatcct acttatacgt ttcatgcttt
    19741 gtttattccg gcgaatgtag ataactggat gtgggatttt ggcgatggta ctacactaag
    19801 cggaaatact gatcctgtac atacgttcac cgcgaatgga aattatgatg tttgtctgat
    19861 cgcaaccaac tcctgcgatt ctgttgctgc atgtttcaac attactatca attccgttgg
    19921 catcgaagag ttgagtcgta caatcaaagt gtatcccaat ctcaccgaag gatgggtatt
    19981 cgttgaatca acaatggaaa atgaagtgat gatggaagtg ttggatgtat ccggacgtgt
    20041 tgtgtatgcg gcatccctgt tcgacagcag aaaaagcatc gatatgagtc cctggcagaa
    20101 tggtgtgtat tttatccgac tcacacaggc aaaccaagtg catgtggtga aagtggtatt
    20161 gcagaaatag tgtaagtaaa aactgcgttg gaaaattatc aggatgagct caggcgatga
    20221 tggcttccga aaaaatcgga catgcttttt tcatcatcat gctttcatcc ggcgattggc
    20281 ttcgatgaga aagggaatgc ttaaaaactt tctgtgagct gcgaattatc agaagtagta
    20341 tcctgatttt tgttgccatt ggtaaaaaac caggcccagc gggtattgat gtaatcccca
    20401 attttatcca gcacgtcgag cactacgttc aacatcattt taatctttat gaatagaacg
    20461 cggctcatag gcatttgttt tacgagttta gggaatggtc gctatacgga ggttttagga
    20521 attccgtgta gggagaaagg ttggctttgg cctgcatctc gatgtagcgg cgtgaaaatt
    20581 cttcctcgtt ttcagaagaa tttaaatagc tgatgcggta atgatcgtaa ttggacagcg
    20641 cctgcttttc atccgtgctc aagcgaactc ccgcgaactc attgtcgcgc aggtcgttga
    20701 cggagatgac cattttaccg gttgatgttt gctgacgggt attctgactc acgatgttgg
    20761 ttttgcacaa aattacgcgg aaggctcgct ttggttgcaa aataaggggc ttatctatcc
    20821 acacgggttt gttaacaatt gttgataacc cttgagggtt ttatcacaga gggatgaacg
    20881 gagggatggg cttggaacgg agggacactt cggcaggctc agtgaaagtg gaaggaggga
    20941 cgtgtggttt tggaacagaa ggatagaagg agggatgggt acttcggcag gctcagtacc
    21001 aaagtatgga tggaagcgca ttcggcactg cgcaaagagg taccgtcgga aatttgtttc
    21061 cggatgatga atctgttggc tgagtggagt cgaagtcaac actaaagaaa gcgacgcaag
    21121 gagctttctg cttcagggtt tccctgcagt atgatgcgat ttgttgtaat ccatcacgac
    21181 gtattgagga ccggtaaacc agtgtttgta gcgcggatct tttgggttgc agccagacca
    21241 ggttccgatg atggaccaga gttgccgcac gcgcaaaaag taactttctt ttttgtgagg
    21301 caaatccctt aaaacaaaaa tgcgatcatt ggtagagggt acgtcgtagt cttcaaaaaa
    21361 ccacaactga aatggagagc cattgactag tcggcgaaga aaaagcggtc gcttgtagtg
    21421 acgcttctga aaccagacat tgtatcctac ctcaacggaa tcggaaaagt accattcggt
    21481 ttgttcgtga cctttaattg taactttttt gatatagcct ttgatgctgc ccggattgta
    21541 aaaacgaaca ctgtcgcccg caaattttgc taccacataa tcgtaaccat aaacaactgt
    21601 tttacgcggc tctcgaaaga cctgtttagg gttgttcgga atgtagcaat agatcgtatc
    21661 gttgttcagg gtgataatgc ggtcgttcat ttcactctcg gcggcggagt gtagaacgag
    21721 gaacaaaaag caaagcgaca gaagtttgtt aaccgatcgc atgaacatga gtatcctgtt
    21781 ttggtgggta aaggtaagaa ggaaaagaac gagtaagaag acgattatca atggatcaaa
    21841 ttccaatgtt ccaatgtgcc gatcctgccc cgaccgtacc tgtcccgacc gtaagcgtcg
    21901 ggaagcgtcg ggatcaccat ttaccgttca ccattcactt cccttcacaa tacgtcttaa
    21961 aattattcag aatcgcctgc caccccgatt tttgtagttc gggagaattt tcgctttcgg
    22021 cgtcgaatac gatggtgatg tttgaggtgt ttccggaggg ttgaaaaaag atatctgctt
    22081 ttcgtccgtc gaccattgtg taggtgatct ttttatgcag atccacttca tcgaagatgg
    22141 cttcaaaatc aaatcccatg ctgccatcgc gtgcttccat tcggctaatg tattttcctc
    22201 caacacgaag atcggattgc gcagaggggc aatgccagtc gggactcgcg aaattccatt
    22261 gggtgatgtg ttgcggttca ttgtagaatt cccagatctt ctcaacgggt tgctctattt
    22321 ttgcttctac ggtgactttt acgatggaca tattcagaaa attaaatgtt gtttttgcta
    22381 gttcagcaat gcaagatact tctttctgtt ttcccagatg atccaggata gaataatcca
    22441 gatgacaatt gcaattggca tgccttcggg cgcaacagtg atatgggtga gcagtacacc
    22501 taccattacc ggaaaaacaa taagtgctcc tagtgcgcgt gttttgggaa acagaatcat
    22561 cattccgcca acaatttctg caaatcccac caatggcatc agccatgaaa tttccatgat
    22621 ggccatgctg tctttaatga gttcttgcgg aagatcagca ggaaccggca tgtaattcag
    22681 aaatttattg agccctccat tcaggagcag taatccgaat aatgcggata ccacgtttaa
    22741 tactttattt ttcattgtgt ttattattta ggttgataat tgatcatcca gttaatgccg
    22801 tatttatcca taaagttacc gaaataggcg ccgaaaaaca tatcctgaat cggaatttca
    22861 actgttccac cttcagaaag agcgtgaaac aaacgatccg cttcttctct cgactcaggt
    22921 tcgatgtgaa tatgcatgtt gtttccgggt gtaactgtga atcccatttc cttcggagca
    22981 tcggttccca tgagaatgtg tccgcctgta ataggaagtt gcacatgcag caccatcttt
    23041 ttcacttctt ctgcaagtgg tggctgaccc tcctgcggtg ggaaatcacc aagacgttga
    23101 atgcctttta caaattctgt tttgaaaatc gatttgtaaa aatgaaaggc ttcttccgtt
    23161 tttccgtcga aattaaggta cgatgaaacg cgaactgcat tatctgtctt tttgtctttt
    23221 cgcagataaa attgtgcagc gatgtaatcg tcgaggtttg caagtccggc tgtaaatcct
    23281 tctttgaatc ccatagtgat gatcgcttca aggtcttcga gcgtttcgaa tttcagcaaa
    23341 cattctacaa cggtactatc atcgtttgca ctgaagccgt tctcccattt cacgcgtggc
    23401 ttgcttgcgc tcattaccgc atgttcatca caaaatgcgt cgatgccgga ataaaattgt
    23461 tggggcagaa ttttttcgta atcaaacaaa caccaatgga tatcaccttc cggaccttgc
    23521 atgtaatagt gccagcgacc tccttcttca aacttcatcg atttggtaac cgatcgatac
    23581 ggcttgggtg cccaccactg atcgaggata tcggattctg tccatgccgc ccacaccaga
    23641 tctatcggcg catgaaagga gcgttttaca tgaatggtat tgtttgtttt gtcggctgta
    23701 aaatcgaata gaattgcctt tttcattttc gtttagtttt taattgttca agtacattat
    23761 ccaattgatc gaagcgtttt tcaagcaatt gtttgaattg ctccagccat ttttcaattt
    23821 ctttcagttt gtttttgttt agatggtaat gcatttctct tccctgttgt tctgctcgca
    23881 ccagttcgca ttcggccagg actttcaaat gcttggagac cgcttgtcga ctactgttaa
    23941 aatgttctgc aatggcattg ggcgttagcg aacctacggc aagcaacaac acaatagccc
    24001 tgcgggttgg gtcggcaatg gcctgaaata catctcgtcg tgcgtccatt tgaagatttt
    24061 ctggcacaaa tatatgcaac tatttggttg cgcaaaattt ttggggtgat ttttttgtag
    24121 gaggatggca gttcacagaa ggagacttcg aggggctcag tgcgggtgga agggatgaac
    24181 agccggttat tgtttgttgt ccgcctttga gctgacttca ttcttcccca cataaaccct
    24241 cagggccact tttacaaagg gagaaaagat ccatgaaatt ccgctggtag ccggaaaagc
    24301 aggattattg agtgaggtgg tgttgatgcg actgattccg ccaccaaaag agagtggtaa
    24361 tgatatgcgt ttggtaaaga aggtttcgta accggcagcg aggtggatgt tggtggatgt
    24421 gcatttactg acgtggcaat gctcctctcc tgtagaggga aagttaatag atcgtggaga
    24481 ggtgcaaata ctttttatat aacgatgcct ttgaatattt aactctgaaa agaaatggct
    24541 gatccgattc agggatgcaa aatgatattt aatgccgcaa tcaaatccgg taacatttat
    24601 cgatgttttg gaaaaatccg gaagaagagc attattcgta ttaaaaccaa agtaaagttc
    24661 gtgagaagaa tattttacaa aagcattcaa tcccgaaaac atgaatttat cgtcctttcc
    24721 aaaggaatag ttgtaattca tttgcggttc tattcctccg ttccattgag attgggcgga
    24781 tgaataaaaa ggtataatga aaattaaaag gataacccga accgacacaa ttataaaatt
    24841 acacgatcaa acaaaccaat tactttatat gatgacatat cttccattgt taagactata
    24901 tctttataag tagaaaatgt tgatcgtggt tttaacacga ttttttcatg cgcccatcca
    24961 tctttgtctt tgctcttaac actggaatac tcttttacgg taaaggaagc acctgtgtcc
    25021 gcatcttgga tatcagaaca ttcaacaagt acaattttcc catttctgct tccaccttcg
    25081 tcctgtttaa agagacaaag agagccgttt ggaatttcct tgttcatcga atcgccaaca
    25141 acctggcata caaagtagcc ctttctggct gcgatattct cagggagttc gacccatttt
    25201 aactctttgt agttctggaa accactaaac cctccagctg ctgcttttat atccaaaaaa
    25261 ggaacagaat taacataagg ttttacttta tcataatcca aaaaccttat tccaatactg
    25321 ggtacaaatg tttcaatgtg ccttttgaga tattctttca tattgggatt gcaggcataa
    25381 atatatgtcc ccctgacgcc cctgaacata attgtcttgt aaatattaat tatataattc
    25441 ttgagttctg tttcatcagc tcctattcga ccgtatttat caaaatactt tgttgcatcg
    25501 atttctattt tattcgtttg ggggttgtaa tcaatttccc atccaaaaat aactcccgta
    25561 aaattcaaat catacccttg cgtggtatgg atacatccaa tctcgttaaa tgcgtttggc
    25621 gagttaaccc agtctatgtg tgttgaattc catttaaatt ttaatccgtc gaaatcaaaa
    25681 tcggtaacat ttgccttggg ctttggcttt ctttttgggt cagataacca ctcccaggca
    25741 tagccggcaa ccattctgga aagaccaaat tgttcttcct ttttcttaag ctcttggtac
    25801 atctgttcaa acgaatcaaa aaacaaaaga tcatatcctc tgggagaaaa aacctgttct
    25861 ttgggtttaa catgcaacaa atcatccaca aagcttatgt aattgttgtt gtcttcacct
    25921 ttaaccctca tttgtgattt caactcaagc tttacgcttt tatcgctatc caatagtttt
    25981 ttaaaatgct tttcagaaat gtcagaaggc ttaacacttt gggctgaatc ataaaagaaa
    26041 atttgatttt tgctgtttgc caaaatccaa tcgagttcat ttccctcatc cccaagtccc
    26101 agtttttggt tgttcgcttt aaacgccccg cgccatgaaa tgttcttata ctgcctcaac
    26161 ctgtgcgact cgtcaacaat taataagtcg tatttctctt ttaacttaaa ggtgtctgcc
    26221 ggactaatta ccattgtggc ttttaaaccg ggcatgtttt taaatacatt ctccagtgtt
    26281 gtcctcagtg aagacattgc aacaaccaat ccaatttttg gatttgggta ttttgatcta
    26341 aatttcctga cataattcaa ctccgttagt tcgttttcat taatgtcatc aagattgtca
    26401 tcgaaattgg tgtttagcaa cttgattaaa taagtagcca aaattgtttt tcctgttcca
    26461 gcagaacctc tcacaaatat ctgatttgac ttttttccaa caagaccatt caaaatctca
    26521 agaacagaat tgaattgatc ttcatttaat gttttgtaag gagaata
//
LOCUS       DAOMYG010000003        26070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_681_length_26070_cov_13.575745, whole genome shotgun sequence.
ACCESSION   DAOMYG010000003 DAOMYG010000000
VERSION     DAOMYG010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 26070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26070
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_681_length_26070_cov_13.575745"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1066)
                     /locus_tag="PK610_00255"
     CDS             complement(170..1066)
                     /locus_tag="PK610_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRJ34382.1"
                     /translation="MSETKPIHSPLKLQVILHVVVFVWGFTGILGQLISVRENVLVWW
                     RMLIAMVALAIFMGISRRSFRLSRRNLLMVLGTGLIICLHWIFFYGAIKISNVSVAVA
                     CMAVASFFTAFLQPLFFKTKMVWYEFILGIGVILGVSVLMGVETDKWEGFVWGLISAF
                     FAALFTVINAGFVKRMDSTVISFYEMTGGFLGLTVYNFFIGALTTDTLALNNSDTWYL
                     LLLGIACTALPFVISVWVMKQLSPYTVSISVNMEPIYTILLAILIWPEKETMSAGFYI
                     GSGIILLTVVLNGYLKSRTNRS"
     gene            complement(1143..1808)
                     /locus_tag="PK610_00260"
     CDS             complement(1143..1808)
                     /locus_tag="PK610_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34383.1"
                     /translation="MKQFSFLLAFALCVSGLNAQKKVEVRESNEKIGGGSNNALTVMI
                     YIDDKDAVEKAWKSKMKDLGAKVSTKEDMFGDDAKTKAMGPNTFDMYARAEVVKGEGI
                     KLIVAVDLGGAFMSSSKHSDQYKVFRDILYEFAVKLTKDHLADNAAAEEKKLSKLEDQ
                     QKDLEKDNKNLKGDIEDYKKKIAEAEDKIKKNEEEQKKKKSEIDEQKKTTEAAKQKLN
                     AVK"
     gene            1935..2381
                     /locus_tag="PK610_00265"
     CDS             1935..2381
                     /locus_tag="PK610_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34384.1"
                     /translation="MKDLLISTHSGLRWLVLLFMVYAVFNAFSKWRFGKAYSGTDKLV
                     NLLTMILFHTQFLVGLLLYFFSDKVQFNGNTMSNSMLRFFTMEHFLMMTAVFVLLTMG
                     RKRAEASETDKAKHRKYMIWYGICLVIVLAAIPWPFRTELAGAWFR"
     gene            2369..2683
                     /locus_tag="PK610_00270"
     CDS             2369..2683
                     /locus_tag="PK610_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRJ34385.1"
                     /translation="MVSLKYSFLVLGISLLFSCTGENESASATPNGKLLFEKNCAICH
                     GKDGKLGQGGAKDLSITSLSKDDMVRIVEYGKPGMTPFKGLLNDGEIQAVVEYIEELK
                     EK"
     gene            2680..3876
                     /gene="hflX"
                     /locus_tag="PK610_00275"
     CDS             2680..3876
                     /gene="hflX"
                     /locus_tag="PK610_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009415691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRJ34386.1"
                     /translation="MIEAKIESRVDETTILVGVITPRQDELQVKEYLDELTFLAETAG
                     AKVIKTFTQKLQVPVPGTYIGKGKLEEITLYVEENDVKLVVFDDELSPSQQKNIQDAV
                     KVPVLDRTNLILDIFAGRARTAHAKTQVELAQYQYLLPRLTRMWTHLERQKGGIGMRG
                     PGESQIETDRRIIQDKIAKLKLQLKEIDRQMSTQRSNRGQLVRVALVGYTNVGKSTLM
                     NILSKSDVFAENKLFATLDTTVRKVVLDNLPFLLTDTVGFIRKLPTQLIESFKSTLDE
                     VRESDLLVHVLDISHPNFEDHFAVVNQTLKEIGALDKPTIVVFNKIDAYRHVEKEEGD
                     LSPIERHNVSLEDLKKSWMGRMSPNKVVFISAEQRINLDELREVLYEMVKEIFAVRYP
                     YNNFLY"
     gene            3953..5179
                     /locus_tag="PK610_00280"
     CDS             3953..5179
                     /locus_tag="PK610_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ34387.1"
                     /translation="MRKFLIYLFLFPLVAFAQEKRVLFIGNSYTQYNNLPQLLRNLAL
                     SGGDSLYTEMNAPGGYTFQNHTTNSITLSKIAQGNWNYVVLQEQSQLPSFPPAQVASQ
                     CYPYARALDSLVRLANPCTETIYYMTWGRKYGDQGNCASYPVLCTFNGMQSRLRTSYL
                     EMGSDNNASVAPAGQAFKLSRERDSTVELYTADNSHPTLEGSYLTACVFYATILKKTP
                     VGLNYTAGINFTTAQFLQQVAADVVLDSVQTWNLNIDHPQPLSTIQVSGNTLNLTDAS
                     TNALSNSWYIEGINYSGTTAQHTFTSNGTYQILQIVQNNCFTDSLVFTVNIGPLNVIS
                     FQPENIRIYPNPVEHLLFVEADNVFCYSVFSADGKLVLFGKSFNAIDVSGLNSGLYYL
                     QLTNDSQQATLKFMRD"
     gene            complement(5176..6087)
                     /locus_tag="PK610_00285"
     CDS             complement(5176..6087)
                     /locus_tag="PK610_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRJ34388.1"
                     /translation="MKKLLSLLFFISSLLAEAQHHPLFSQYFFNDFFINPAVAGTRDW
                     YDVRSNHRYQWVGLTDAPRTFTLSAHGPNKKYNMGFGGSIFTDNVGPTRRTGFNFSYA
                     YHLKINKDVKLSLGVSAGMLQFMIDGHKITTYDPGDAVISNGLQWSMEFDAQFGFYLY
                     HKKWFVGGVIPQLLRNKLYFFSNQLTTGSNLLYHYNFTAGYKFDVGDDFQIEPSFLVK
                     YVKPIPVQLDLMARAIYKEQVWLGASYRTMDAVSIMAGYQYKNYLSIGYAYDITTSNV
                     RNYSSGTHELMLGVRFVRRENKTKTIL"
     gene            complement(6091..16083)
                     /locus_tag="PK610_00290"
     CDS             complement(6091..16083)
                     /locus_tag="PK610_00290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ34389.1"
                     /translation="MRRFLQSLFFLNFMLLVQFSNATAYYWIGGTGNWSDASHWSLQD
                     GGAGGAGVPGVNDDVFFTSGSFSSSKNVIRFDVSASIRNFNFSAQNSFPRFQSDGKIN
                     FTITGNWIVTGQFQNHINGNIRFNPSQPNAILQTGNVHFRANLIKQGYNSLSLQNNDL
                     ELEPGFDLVLESGAFFTSGKGVKADQILFSSNQQVVFNSSGSSLFTLEPIDPAGTLVN
                     WIANGTEQFIIRNTDTENPQLRLTATCGTAPNQFTINAQVVTNYNGQHVSCKGANDGE
                     VCVTITGAPGPFNIQWLGGGPATSCWTGLGAGTYTVRVINTGVLPNVICATTVTIVEP
                     GDLTVLSWSTTDPSCNYTCDGTATPFVFGGTPGYTYTWSSGETAPVATQLCVGLNTMN
                     VVDANGCTFDTTFFILTPLQISPNLITTNLACFGVCNGSVESQPTGGNGAPYSYSWSN
                     GATTALNPNLCAGNYSVTVSDNAGCTVTASATVTQPQPIIVTLTNQTNLICNNVCSGS
                     LTVNAAQGVGNYSYQWFTSPGNTPIAGQTSATASGLCAGSYYVVVTDDNGCSQTSAVF
                     TITQPTPVTASTSFTPPLCHNECNATLSGIANGGTPGYVFTWINAATATPIGVGNPLN
                     GVCPGTYFAQVQDGNGCLINTIPPMTIPNPPALALLVNPVNTVCHNDCNGSSSVVSAS
                     GGTGALTVSWLSGATVLGTGNSITGLCDGTYTARVTDANGCVYDTTFVIAEPAPYSFS
                     LTTTPVSCVGTCNGTAVLSGISGETSPYSVSWSSSGNTGLTENNLCSGNFLVTIIDVN
                     GCDTIHPFTITAPPVLSLNPSFTEPSCAGVCDGTATAGPAGGTLPYTIAWFEQPGNIL
                     NGQTSDPATGLCDGNYSVTVTDGLGCTATASYTLTDPITMVVASSSTTTSCGICDGTT
                     TVNIIGGTAPYSVVWMNATTMTPTGQTGITATGVCAGNYFAIITDANGCSVNSPQVTV
                     SPNVIITASAISVSPSCFGFCNGMVDVTVSGGTAPFTFQWFDQTTNLPIGQSTEDAIG
                     LCAGTYYVTITDVNNCSPAPLVVTLTQPNQLTATANGINPTCFGVCNGTLSGTASGGT
                     APFSFSWVDVATGVIVSTLQNPNGICSGTYALTVTDALGCSAGPHNVTLVAPASIVVQ
                     LNSSNALCFNQCNGQATVTVAGGTAPYTATWSSSGNTALTETGLCDGNYTVTVTDANG
                     CASTPQNFSISEPLLLSATTTNATVLCNGDCNASVSVAPVGGTAPYSYQWNAAAGNQT
                     TPVANNLCAGSYSVIVTDANGCTTGPLNSFVTQPTALSGSATATPASCNGICNGTATV
                     APAGGTAPYTFSWSDPFSQNTITAISLCGGSYSVIITDANGCSFTPSAVTVTEPSVLT
                     VTASGTNATCFDVCNGTATVSIVGGTAPFAIVWNDLAAQTSLTATGLCDGNYNVQVTD
                     ANGCSGNATATITEPTAITATSSSVQATCGVCNGSATVNPSGGTGILSILWSVNASAQ
                     TTPTASGLCAGIYDATITDANGCTEVITVGVSDNTNEILDVAATQVSCFGDCNGTVSV
                     NVTCADPTCTLVWNDPAASTTNTVTGLCAGTYAVTVTNGLGCITIATVDVIEPPLLTL
                     SMSNTDVLCFGTCTGTGTALPAGGTAPYSFAWNAAGQTTSTSFNLCPGTFSVVVTDAS
                     GCTVNGSVIIDEPSPLSATIASSDADCQGQCNGTGTVFPSGGISPYTFLWDDPASQTT
                     QQATALCGGQYQPTVFDANGCTFIPPQITIQEPDQISLVLNASPLDCNGDCNGIATSS
                     VTGGTAPYFYQWNDPLSQTTANASNLCAGNYELIVTDVNGCIGGPGTVAIATPVALNV
                     SFSVSSPLCSGQCNGSVSATVIGGTQPYTYQWNNPSASTSATVSGLCAGFYTLIVTDA
                     NGCSTTASVAIDDPMQLILPLTFGNVSCFGSCNGFVTSNPIGGTAPYSYLWNTGATSP
                     GVSGLCPGLYSVVVRDANGCVVSGSRNIIEPAQLSVTSTFSPSTCGVCNGIASVSPSG
                     GTPAYSYQWNAAAGNQTTQAANGLCAGVYSVVVTDNNGCTEQLAVAISDLGAETVSTA
                     VIDATCFGQCNGSATAITACVNGPCAFEWYSGITGVAIGQTNAIASGLCAGNYFVEVT
                     NALNCVTIVPAVIGEPTEITANAVASQPSCNGDCNGSAIVNPSGGTGPYTILWDAAAG
                     GGNSATVNNLCSGTYIVTITDATGCSDPDTVILINPTILQASAAAVDVACSGNCNGSA
                     LLTISGGNAPYLINWNDPFVQTSLNAVGLCAGSFIASVQDANGCVTQANAVISEPAAL
                     IVTMGISDNLCFGDCTGQATAIANGGTSPYTYIWNDALNQTSATATGLCPGNYQVIVT
                     DANTCTVTTFPVAITNPTQVSISVTATDVACNGSCNGSITVTPSGGSGPYSFSINGGL
                     TFQAGNTFNGLCAGSYEAVVMDANNCLSVIQNININQPTQLTATDQIFPADCNTPNGA
                     ATILPLGGTPLYTFVWMDQLLNPIGQTTQTAINLSAGVYVAEVTDANGCVAQVSVTVN
                     NVISPTVTPVVTNVDCNSNCNGAIDITVTSGAAPFSFFWFPGGQISEDLTGLCAGGYL
                     LQVTDAAGCIGFANLSVTQPAQLNASFIVTDANCGQCDGAASVTASGGTGSYVFDWSN
                     GQTGSAASSLCAGAYMVMVTDNSGCSANFNVPVNNIGGPNGENVTVTNPVCNGDCNGT
                     ISLNPIGGTAPYSFYWIHDGATINTSNNLCAGTYYLEVTDANGCVRVSTIDLIEPASL
                     NDSLLVLPSTCGNCDGTLSVFVFGGTPPLSYQWNAAAGNATTSLVSNLCAGIYEVEIT
                     DGNGCATSQFYTLNDLTAATLSLSAQNVSCQGNCDASSTALVSGGAGPFSTEWFLNDG
                     TSTGQSGTTAVNLCAGDYIVEVTDAAGCIAFEDITITEPDSLLFALPFVQAPSCFNSC
                     DGIAVAIVIEGSLPYVFSWNDPASQSTATASGLCTGTFIATVTDANGCSTNQTIVVPA
                     PAAIVLSLDSTDASCSTVADGAINATVSGGAGNFTFNWTGPNGFTEATEDIANVFSGI
                     YYLTVTDANGCSLTDSIIVNAILVVNAFAGNDTTICAGSLLGVSLTGSGGVTYEWFDM
                     IGVSLGTSQTVNVNPQFGTSDYILVANNNGCIDSDTVSVSVNTLPSVSAGPDIDIVAN
                     TSTVIGGSPTTSAGNSVLWVPSYHLSDSLALNPVASPDSSTNYIVFVTDANGCVNSDT
                     MRVQVFPFISFPNGFSPNGDGMNEVWQIDFIRLFPEAQVEIYNRWGQLLFLSIGYNTP
                     WDGTFNNQPVPVGTYYYIINLNHPLYPDAFTGPLTVIR"
     gene            complement(16208..17146)
                     /locus_tag="PK610_00295"
     CDS             complement(16208..17146)
                     /locus_tag="PK610_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRJ34390.1"
                     /translation="MMKRIFTGAVFVVLFLQSSAQQTPQFTQFVFNQFAHHPAVAGSR
                     PCLDMRVGYRTQWVGFDGRPITMFANAHGQIMGKNRKHLKTKHGIGGHVESDGAGPIS
                     RTKIYLSYAYHIPVGRKTNLGMGLYAGFQQLRFNAGKVTLANPNDNSIPGSSVQFVWP
                     DIAPGLFLYSEDYFVGFTMWQALRNKWKNVGDQSRLTHHFVLSGGKRFKYSDDLSWIP
                     SAALKFAPMSTPAIDLNLMMDFQNKMQVGLSYRNTDAICALFKLNFLKHFSMGYSFDF
                     TTSRIRQSSSNTHEIILGISACPHRGKGITDCPAWN"
     gene            complement(17143..19392)
                     /locus_tag="PK610_00300"
     CDS             complement(17143..19392)
                     /locus_tag="PK610_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ34391.1"
                     /translation="MMKWIVQLVFLFPVIGLYAQPANNGCQNAQVLCPGAAFAGSNIG
                     SDSGPEDGTGFSCFGLNNSVWYSFTTNANGGDVNVSITGINCIPGAGADLELQAVLLS
                     AGTACNAATYTEIDCNAGSATSIQLNGTGLSPNTTYYILIDGDSTGAGVTQAAQGTFS
                     IEVNGSAVEWQSTSAVTDQTCGNLDGEIDITAIQGGTPAYQYSLNGGAFQSGTQFTGL
                     AAGSYVLRVIDASGCIQFVDTIVVNLLNGPENGTATTIDAGCNTNDGTFQVTGVTGGT
                     APYTYSLNGGPPQAGDIFNPLPAGNYSVIVSDQQGCTQTVNGVISNAGGPDNALPTII
                     HPDCNATNGSFTVAVTGGTAPYTFSLNGGAAQLSNAFTNLAPGTYSVLVTDDNGCTFL
                     LSGISLVEQPGNLVPSVVLTASPNPACPGDNVTFTAVVQNAGTNPALTFTVNGAVVQS
                     GPATSFSSGTVANGDVVVCSVTSSDPCLAVLSTNSNSVAVVVNPQIDPTVSIVSNTTT
                     ACSYDAVTFTATQNGCTGTANYQWLINGAPFATNTTGVISSTFASDVTVSVNMSCTDP
                     CSSTASSNAIDLDIIEVNANAGADQIIAPGQTAVLSGSGGGTYNWTPTSSLSNSTSAT
                     TFATPGNTTVYTLTVTDNGCFDQDQVVVYVVDPISIPNTFTPNGDGTNDTWQIQNIEN
                     FPNARVTVYDRWGQKVFNRQGYSNNNAWDGTNKGMKIPAGVYYYVIDLNTGSDDENSQ
                     YSGSITIVL"
     gene            19533..>26070
                     /locus_tag="PK610_00305"
     CDS             19533..>26070
                     /locus_tag="PK610_00305"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34392.1"
                     /translation="MNNIYKTLKSNQLTLLFCLLLAFSGTVRSQHINYQNDSGWKIGF
                     NMGGVWQQSDMKSKAGIGFGATLGKALYQKPGRYFAFDLRFRYLHGYNYGYTDTAITQ
                     FPATSIYSGNNTANPTLNAYQSQGKMFYNYRMALNEYSMEGVLTFNRLRERTGIVLYT
                     WGGIGLTTYRVKTDQIDNPFLGSGTPYNYTAFDSTSTWSQLSGIWDGEYETDADLNRG
                     LQVGFMPSWGIGFGYQFSPYFAMVLEHKVTFALNDKIDGYAYKNPETPVNDRYHYTGL
                     KFVFHFGKGNGQVYTNYNTTTTTTTTATTSTSTVVTTPPRARPIINITQPSMSPATVS
                     NQYYTVNANIYNIDGPSNINFKVNGNQSTNFTYNTSTQQFSSSILLNPGSNVVEIRAT
                     NEVGSDYQSVVIVFDMPIINNIPPPIVTFTNPPFTPYNTNNQSFNVTATILNVANSSG
                     IAFKVNGQTVSGFSYNPTSRAFSAVIGLHAGPNTVEIKATNTSGMDVKQVVINYQPVV
                     VQQPPVVTITNPTLNPYTVYVNSANVDATVLNVSNSSDIRLRINGQLSPNFTYNTTTK
                     VMNVIASLIPGPNTFEITATNAAGTDSKSTTIVYVAPSALPPVITFIDPATNPATVTT
                     STKTVRATVQNVAGQSNIQVRLNGIIVPNFSWNASTKVVQFNATLNPGANTVQITATN
                     TVGSDIKATTIIYQQVQTGQPPIVSISTPAQNPYATTSPNASIIGSVLNVNSQSQITF
                     KVNGVPSNSFTYDAVTKVFLANVTLNPGTNVYEISATNNFGSDSRTQTINYTLPCNAP
                     VITLVQPASANFQTSLSNLPFTAIVQNIGNQSAITFKLNGNAHPFTYNAASGTVTATL
                     NLSGTSNTVELKATNNCGMATQSILIYYQAPLVPPIVTITTPATNPFTSANASTAITA
                     TIQHVSSAANVSLKVNGTSVTGFTFNPQTTLFSANVNLISGANTIEITGTNNDGSDSK
                     STVIIYQPVAPPCQQPVIVLTQPAGNNYSTALSSLQVTANILNVTTSTGIVFRQNGTN
                     VPFTFNSATGAFSANVNFTQPTNNLEIIATNSCGSSTSTISVTYNRPLVPPVVTILNP
                     GQNPFNSAVANMTLTANILNITGSSDITYTINGVSSTSFTFNPTTNVFTSNVTLASGN
                     NVFTISATNADGSDSKSTTIIYTPVVVPCNAPVITPIAPATASQTVNLANYSIQASVL
                     NATQSQITLKQNGVSKTFTYNPTTFLLTASVTLVDGTNNFEILATNNCGSETTSFSID
                     YNRPLVPPVVTITQPAADPYTSATQNTTITASILHINGSSDISFKVNGVPSTSFTYNA
                     TTKVFTSAITLTPGNNSVEIIATNTDGTANDSRTIIYQYVAPCNPPAATITSPAANTV
                     VAVPFATFTATVTNSSQNAISLKLNGTTVPFTYTNGTVSANLTLVEGGNVVELTAGSN
                     CGTASESVTITYNRPLTPPTVTISAPQNGSTTQTSTSTVNATITNIQNTSGIVFLVNG
                     NPSTSFNFNAANNSFTASVSLNPGVNTFVITATNQDGQASDQTSITYQPCIAPQIVLS
                     QPSATGTTVANAAFNLVAMVQNGANTTNSVTLNGANVSATSTGNAINSGLTLVAGTNT
                     IVINVAGPCGSDSETITIIYTPCFDPEIQLNQSGVNGSTVSSSAFAFSGTVANASGTS
                     LSVTFNGSNANATYSNGSFNGNLTLAQGLNTIVINVTGPCGTDSKTIQITYTPCNAPL
                     ITINPNSGGNNVANAAYTITGTVANNTGTTVAITLNGTAVTSTYSAGSFSAGLNLNSG
                     TNTIVVTVTGPCGNATQTVNVTYTPCNAPVITLNQANANGTDQSNASFALSGSVSNHS
                     GTTVSVQLNGAAVNATYNNGNFNASLILAAGSNTILVTVNGPCGTDTKTISLTHTPCV
                     APVITMTSPSSTGITVSNPSFTVIGSVAAGLNNVIKVKVNGTEVFTTSTANSVNTNVT
                     LNQGSNNIEILVNGDCGSAQSAFAVTYTPCNAPVVNVAQSSANNSTQTAATFSLSGSV
                     NNYQGTTLTVQLNGGTVNSTYTNGNYSAGLTLVPGSNNIVITATGTCGTDTKTLTINH
                     TPCVTPLIGITNPSAASTSSTTASFTVSGAVTGGLNNVVTVKHNGTEVYQSTTSNNIN
                     VPLTLTQGSNTILVSVTGDCGNASQTYTVNYTPCNAPVVTITQPAAA"
BASE COUNT         7786 a         5942 c         5278 g         7064 t
ORIGIN      
        1 gtggaccatt aacaggataa gtgtcctgag ctgaaacggc gaaagccaaa agaaataagg
       61 tggcggtaaa aagtcttttc atgaaaaaag tagtctggaa caaaaataac attcaaacaa
      121 tacaaaaggt tgactctggt acaaacggaa tcaatgcagg aagagtgggt caggatctgt
      181 ttgtccgtga tttgagataa ccattgagca ccactgtcag caatatgatg cccgaaccaa
      241 tgtaaaaacc agcgctcatg gtctcctttt caggccagat caatatagcg agcagaatgg
      301 tgtaaatagg ctccatgttc accgaaatac taacggtata gggcgataat tgcttcatta
      361 cccatactga gatcacaaat ggcagcgcgg tgcatgctat tcccaacagc agcaggtacc
      421 aggtatccga attgtttaac gccaaggtat ctgtggttaa tgcgccgata aagaaattat
      481 acactgtcaa tccaagaaac ccgccagtca tttcgtaaaa ggaaattact gttgaatcca
      541 tccgctttac aaaacctgca ttaattacag tgaataatgc cgcaaaaaat gcagagatca
      601 atccccatac aaatccttcc catttgtccg tttccacacc catcaaaaca gaaacgccaa
      661 ggatgactcc tattccgagg atgaattcgt accaaaccat ttttgtttta aagaaaagtg
      721 gctgcaaaaa agcagtaaaa aaacttgcta ctgccataca ggcaacggcc acagacacat
      781 ttgaaatttt aattgcaccg taaaaaaaga tccagtgaag acaaatgatc agtccggtcc
      841 ctaataccat taacaggttt ctcctgctaa gccgaaaact ccggcgcgaa atacccataa
      901 aaattgccaa agcaaccatg gcaataagca ttctccacca cacaagcaca ttttcccgaa
      961 cgctgattaa ttgtccgagt attcctgtaa agccccaaac aaagaccacg acatgcagta
     1021 ttacctgcag ctttaaaggg gaatgaattg gttttgtttc gctcataaaa aagaaaaagg
     1081 gccatcaaaa atagccctta aactgtaatc gtgaatttta tttttgaata accagcggaa
     1141 tcctatttta cagcgttaag cttttgtttg gcagcttccg tggttttttt ctgttcatca
     1201 atttctgact tctttttctt ttgttcttct tcgtttttct tgattttgtc ttctgcctca
     1261 gctattttct ttttataatc ttcaatatcc cctttcaaat tcttattgtc cttttccaaa
     1321 tccttttgct gatcttcaag ttttgagagt ttcttttctt cagcagcggc gttgtcagcc
     1381 agatgatctt tcgttagttt cacagcaaat tcgtaaagga tatcccgaaa tactttgtat
     1441 tgatcgctgt gttttgaaga agacataaag gctccaccaa gatcaacagc aacgatcagt
     1501 tttattcctt cgcctttaac gacttctgcc ctggcgtaca tgtcaaatgt attcggaccc
     1561 attgctttag ttttggcgtc gtcgccaaac atatcttctt tggtggaaac ttttgcacca
     1621 agatctttca ttttgctttt ccacgccttt tctacggcat ctttatcatc aatatagatc
     1681 attacggtta aagcgttatt gctaccgccg ccgatctttt cgttggactc acgaacttca
     1741 actttttttt gtgcattcag cccgcttaca cagagtgcaa atgccaatag gaaggaaaac
     1801 tgtttcatat ttagcttgtt tgcttaaata tacacaaatc ctgttccaaa agcgaaagaa
     1861 aaacgaaatc actgaatttc agccgcaagg gctcgacctg tcctcctttt gctatttttg
     1921 taaaaaacca cattatgaag gatcttctta tttccaccca ttccggtctg cgttggctgg
     1981 tgttgctatt catggtctat gctgttttca atgcattcag caaatggcga tttgggaaag
     2041 cttattcagg cactgataaa ttggtgaatc ttttgacgat gatccttttt cacacccaat
     2101 ttctggttgg attgttattg tattttttca gtgataaagt acaatttaac ggaaacacca
     2161 tgtccaattc aatgctccgc ttttttacga tggagcattt cctgatgatg acagcagttt
     2221 ttgtattact aacaatgggg cgtaagcgtg cagaagcgtc agaaacagac aaggcgaaac
     2281 atcgcaaata catgatctgg tacggaatct gtttggtaat cgttcttgct gcaattccct
     2341 ggcctttcag aacagaactt gccggagcat ggtttcgtta aaatatagct ttctggtatt
     2401 ggggatttcg cttctgtttt cctgtaccgg agagaatgaa tctgcatcag ctactccgaa
     2461 tggtaaatta ctgttcgaaa aaaactgtgc gatttgtcat ggtaaagacg gcaagcttgg
     2521 tcaaggcggc gcaaaggatc tctccattac tagtctttcg aaagacgaca tggttcgaat
     2581 tgttgagtac ggaaaaccag gaatgactcc ctttaagggc ttgttgaatg atggtgagat
     2641 tcaagctgtg gtagaatata tagaagagtt gaaagaaaaa tgatcgaagc aaagatcgaa
     2701 tcgcgtgttg atgaaacaac cattcttgtt ggagtgatca caccccgaca ggatgaactt
     2761 caggtaaagg aatacctgga tgaactgact tttctagctg aaacagccgg agccaaagtc
     2821 ataaaaacat ttacccaaaa acttcaggtt cctgtacccg ggacctatat cggaaaaggg
     2881 aagctggaag aaataacgct atatgttgaa gagaacgacg taaaacttgt cgtgttcgat
     2941 gatgaattat cgcccagtca gcaaaaaaat attcaggatg ctgtaaaagt tcccgtactc
     3001 gacagaacaa atctaattct ggatattttt gcgggtagag cacgaactgc acatgcgaaa
     3061 acacaagttg aattagccca ataccaatac ctattgccaa gattaacccg aatgtggacc
     3121 cacctcgaac gacaaaaagg tggtatcggt atgcgtggtc ccggtgaatc gcaaattgaa
     3181 accgaccgca ggattattca ggataaaata gccaaactca aacttcagct caaagagata
     3241 gacaggcaaa tgtcgacaca gcgcagcaac agaggtcaac tcgttcgcgt cgctctggta
     3301 ggttatacca atgtggggaa atccacgctg atgaatattt tgagtaagtc ggatgttttt
     3361 gcagagaaca aactatttgc aacgctcgac accacagtga gaaaggttgt gctcgataat
     3421 ttaccctttc tcttaaccga cactgttggt tttatacgca agcttccgac ccagcttatt
     3481 gaatcattca aaagcactct ggatgaagtg agggagtcgg atttattagt gcacgtattg
     3541 gatatttctc atccaaattt tgaggatcat tttgcagtag tcaatcaaac actgaaagaa
     3601 attggcgcat tggataaacc aacaattgtt gtattcaaca aaattgatgc ctaccgccat
     3661 gttgaaaaag aagaaggaga cctctcgccc attgaaaggc acaatgtttc gcttgaggat
     3721 ttgaaaaaaa gctggatggg caggatgtcg cccaataaag ttgtgtttat ttcagctgag
     3781 caacggatca atcttgatga gcttcgcgaa gtattgtatg aaatggtaaa ggaaattttt
     3841 gcagtacgct acccttataa taatttcctc tactaaacag cattccaata ttcgctttat
     3901 tttttgtggg caaccctttt cgttttacgc gttatccatt aaacttcaag gcatgcggaa
     3961 atttctcatt tacctttttt tgtttccact ggtagcgttt gctcaagaga aacgggtatt
     4021 gttcattggt aattcctaca cccaatacaa caatttaccc caactcctca gaaacctcgc
     4081 attatcaggt ggtgattcgc tttacactga aatgaatgcc cccggaggtt atacctttca
     4141 aaatcacacc accaattcaa ttacactttc aaaaattgcg caaggcaact ggaattacgt
     4201 agtgcttcag gagcaaagcc aactaccttc atttcctcct gcacaggttg ccagtcaatg
     4261 ttacccgtat gcaagagcgc tcgacagctt ggttcgtttg gcaaacccat gcacagaaac
     4321 catttattac atgacatggg gaagaaaata cggcgatcag ggaaattgtg cttcctatcc
     4381 cgtattgtgc acctttaacg ggatgcaatc acgacttcgc accagttatt tggaaatggg
     4441 ttcagacaac aacgcaagtg tggcgccggc aggacaagca tttaaattat cccgggagcg
     4501 agacagcaca gttgaattgt atacagcaga taatagtcat cccaccttgg aaggaagcta
     4561 cttaactgca tgtgtttttt atgcaacgat tctgaaaaaa acaccggttg gtttaaacta
     4621 tactgcagga ataaacttta cgaccgcaca gtttttacag caagtcgccg ctgacgttgt
     4681 tttagatagt gttcaaacat ggaatctgaa tattgatcat ccgcagccac tcagcacaat
     4741 tcaggtttca ggaaacacac ttaatttaac ggacgcaagt acaaatgcgc tatcaaattc
     4801 ctggtatatt gagggaataa actacagcgg gacaacagct cagcacacat ttacctcgaa
     4861 cggaacctat cagatcctac aaatagtgca gaacaattgt tttaccgaca gcctcgtttt
     4921 tactgtgaat atcggaccgt taaacgtaat ttctttccag ccggaaaaca tccgaatata
     4981 tccgaatccg gttgagcatc tgctttttgt tgaggcagat aatgtattct gctattcagt
     5041 gttttcagca gacggaaaat tggttctttt cgggaaatct tttaatgcaa ttgatgtatc
     5101 cggattaaat tccggtttgt attatttgca attgaccaat gattcgcaac aggctacatt
     5161 aaaatttatg cgcgattaaa gaatagtttt tgtcttattc tctctgcgta caaaacgaac
     5221 gccaagcatc aattcatgtg tgcctgagct gtaattacga acattggaag tagtaatatc
     5281 gtaagcataa ccaatgctga ggtaattttt atactggtat cccgccatta tggaaaccgc
     5341 atccattgtg cgataagaag cgccgagcca aacctgctct ttataaatgg cccgagccat
     5401 taaatcaagc tgaacaggaa ttggctttac atactttaca agaaatgaag gttcaatctg
     5461 aaaatcatct ccgacatcaa acttatatcc ggctgtaaaa ttataatggt ataataaatt
     5521 gcttcccgtt gtaagctgat tggaaaagaa gtagagtttg ttgcgcaata attgtggaat
     5581 aactcctcca acaaaccatt tcttgtgata gagataaaac ccgaattgag catcaaactc
     5641 catcgaccat tgcaaaccat tactgattac tgcatcaccg ggatcgtatg ttgttatttt
     5701 atgtccatca atcataaact gcaacatccc tgcagaaacc cccaaactca atttcacatc
     5761 tttatttatt ttgagatgat aggcgtaaga aaaattaaat ccggtacggc gtgttggtcc
     5821 aacattatct gtgaaaattg atccgccaaa acccatattg tattttttgt ttggaccatg
     5881 tgcacttaga gtaaacgttc gtggagcatc agtaagacct acccattggt aacggtgatt
     5941 gcttcgcaca tcataccagt cgcgcgtgcc tgcaacggca ggattgatga aaaaatcatt
     6001 gaagaaatac tgactgaaaa gcggatgatg ctgcgcttca gccagaaggc tcgagatgaa
     6061 aaacaagagt gataataatt tcttcatagc ctatcgaatt acagttaatg gtcctgtaaa
     6121 tgcatccgga tacaatggat gattcaggtt aatgatataa taataggtac caacaggaac
     6181 aggctgatta ttaaaggtac catcccacgg cgtgttataa cctatagata aaaacagcaa
     6241 ttgtccccaa cggttgtaaa tctcaacttg agcttccgga aacaaacgaa tgaaatcgat
     6301 ctgccatact tcattcattc catcaccgtt tggcgagaaa ccatttggga aggagatgaa
     6361 aggaaacacc tgaaccctca ttgtatcaga gttaacacag ccgtttgcat ccgtaacaaa
     6421 cacaatataa ttagtagagg aatccggaga agcaacagga ttcaatgcca gtgaatcgct
     6481 cagatgatag gacggaaccc aaagaacaga attaccggca gatgttgttg gactaccacc
     6541 aatcactgta gaagtatttg caacaatatc gatatcggga ccggcactta cactgggcaa
     6601 ggtatttacc gaaacagaaa ccgtgtctga atcaatacag ccattgttat tggcaaccaa
     6661 aatataatcg cttgttccaa actgtggatt aacattcaca gtttgcgatg ttcctaaaga
     6721 aacaccaatc atatcaaacc attcgtaggt aacaccaccg ctacccgtta aggaaacacc
     6781 aagcagactg ccggcacata tcgtagtatc attccccgca aatgcattga ctacaagaat
     6841 tgcattcaca atgattgaat ctgtaaggga acagccgttc gcatcggtaa ctgtgagata
     6901 ataaattcca ctaaacacat tggcaatatc ttctgttgct tcagtgaatc cgttggggcc
     6961 tgtccagtta aaggtgaaat tcccggctcc acccgaaaca gttgcattga tggcgccgtc
     7021 cgcaactgtt gaacaagatg catctgtact atcaagcgaa agcactattg ccgccggagc
     7081 aggcactaca atggtctggt ttgtgctgca accatttgca tcggtaaccg ttgcaatgaa
     7141 tgtgcctgtg cacaatccac tggcagtagc ggtagattgc gaagccggat cattccacga
     7201 aaagacataa ggaagcgagc cttcaatcac aattgcaaca gcaattccat cgcaagaatt
     7261 aaaacatgat ggcgcctgaa caaatggcaa ggcaaacaaa agactatcgg gctctgtgat
     7321 ggtaatatct tcaaaagcta tacatccggc tgcatccgta acttcaacaa tatagtctcc
     7381 tgcgcaaagg tttacagctg ttgtgccgga ttgtccggta gaagttccgt catttaaaaa
     7441 ccattccgta gaaaacggtc cggctccacc ggaaacaagg gccgttgatg atgcatcaca
     7501 atttccttga caggagacat tctgtgcaga taaggaaagc gtagcagcag tgagatcgtt
     7561 taacgtatag aattgggatg tagcacaacc gtttccatct gtaatttcta cttcgtaaat
     7621 gcccgcacaa agatttgaca ccagagaagt agtggcattt ccggcggcag cattccattg
     7681 ataggacaaa ggaggagttc ctccaaatac aaatacactt agtgttccgt cacaatttcc
     7741 gcaagtggat ggaagcacca gtagagaatc attcaatgaa gcaggctcaa taaggtctat
     7801 tgtacttaca cgtacacaac cattggcatc agtaacttcc aggtaatagg tgcctgcaca
     7861 caaattattt gaagtgttta ttgttgcacc atcatgtatc caataaaaag aatagggcgc
     7921 tgttccgcca atgggattta aactaattgt tccattgcag tcgccgttac aaaccgggtt
     7981 ggttaccgtt acattctcac cgttgggccc gccaatatta tttaccggaa cattaaaatt
     8041 tgcagagcac cctgaattgt ctgtaaccat taccatgtat gcccctgcac aaagtgaaga
     8101 tgcagctgat ccggtttgac cattgctcca atcgaaaaca tacgatccgg ttccaccgct
     8161 tgcagtaacg cttgccgcac catcacattg accgcaattt gcatcagtta ctataaatga
     8221 agcgttcagt tgcgccggct gtgtaaccga taaatttgca aaaccaatac aacctgcagc
     8281 atccgtaacc tgaagaagat agccacctgc acacagtccg gtaaggtctt cgcttatctg
     8341 tccaccagga aaccagaaga aactaaaggg agctgcccct gatgttactg taatatcaat
     8401 tgctccattg caattggagt tacagtcgac attggtgaca actggagtaa cggttggact
     8461 aattacatta ttaactgtaa cgctcacctg agctacacaa ccattggcat cagtaacctc
     8521 agcaacatac acgcctgctg aaagattaat ggctgtctgt gttgtttgac caatgggatt
     8581 tagtaattga tccatccaaa caaaagtata caagggtgta ccccccagtg gcaaaattgt
     8641 agcagcgcca tttggtgtat tacaatccgc agggaaaatc tggtcagttg cggtgagttg
     8701 tgttggctga ttaatgttga tgttttgaat gacagacaaa caattgttcg catccattac
     8761 aacagcttca tatgaaccgg cacataaacc attaaaagtg tttcctgctt gaaaggttaa
     8821 tccaccgttt atactgaaag agtacggtcc acttcctcct gaaggtgtta ccgtaataga
     8881 tccgttgcaa cttccattgc aagccacatc agttgcagta acagaaattg aaacttgtgt
     8941 aggattggta attgcaacgg ggaatgtggt tactgtacag gtattcgcat ctgtaacaat
     9001 aacctgataa tttccgggac aaagtccggt agcagtggcc gaagtttggt tcagtgcatc
     9061 attccagata taggtataag gtgaagtgcc tccattcgca attgcagtag cctgaccggt
     9121 acaatcacca aaacacaaat tatctgaaat tcccattgta acaatcaatg ctgcaggctc
     9181 agaaataact gcattagctt gtgttacaca tccatttgca tcctgaacag aagcaatgaa
     9241 tgatcctgca cacaatccaa cagcatttaa agaagtctgc acaaaaggat cgttccaatt
     9301 aatcaagtaa ggagcattac ctccggaaat agtaagcaaa gcggagccat tgcaatttcc
     9361 tgaacatgcc acatcaactg ctgctgcaga tgcctgcaaa attgttgggt tgataagtat
     9421 taccgtatcc ggatcagaac atcctgttgc atctgtaatt gtaacaatat atgttccgct
     9481 gcaaaggttg tttacagttg cagaatttcc tccaccagct gctgcatccc acaaaatggt
     9541 gtaaggtcca gtgccgccgg atggatttac gattgcagat ccattgcaat cgccattaca
     9601 ggaaggttgt gatgcaactg catttgcagt tatttctgtt ggttcaccaa ttactgccgg
     9661 aacaatagtt acgcaattca gcgcattggt aacctcaaca aaataattac cagcacacaa
     9721 tccacttgct attgcatttg tttgtccgat tgcaactccg gtaattccgc tgtaccattc
     9781 aaatgcgcaa ggaccattaa cacatgctgt gatcgctgtt gcagaaccat tgcactgtcc
     9841 gaaacaagtc gcatcgatta cagcggttga aacagtttca gcaccgagat cactgatcgc
     9901 aactgctaat tgttcggtac atccgttatt atcagtaacc acaacagagt aaactcctgc
     9961 acataatcca tttgctgcct gggttgtctg atttcctgca gccgcattcc attggtaact
    10021 atatgcgggt gttccgccgg aaggactcac agaggcaata ccgttgcata ctccgcatgt
    10081 agatggagaa aaagtactgg ttactgagag ttgtgcaggc tcaataatat ttctgcttcc
    10141 tgaaactaca catccgttcg catcacgaac aacaacacta tataatccag gacacaaccc
    10201 acttacccca ggtgaggttg ctcctgtatt ccataaataa gaataaggcg cagttcctcc
    10261 aattgggttc gaagtgacaa atccattgca tgatccgaaa caggaaacgt ttccaaatgt
    10321 caatggtaaa atcaattgca tcggatcatc aattgcaact gatgcggttg ttgaacatcc
    10381 gtttgcatcc gttacaatca acgtataaaa acctgcacac aatcccgaaa cagttgctga
    10441 tgtgcttgct gaaggattat tccattgata ggtataaggc tgtgttcccc caataacagt
    10501 agcagaaaca cttccattac attgacctga acacaatgga gaagaaaccg aaaatgaaac
    10561 attaagcgca acaggtgttg caattgccac tgttcccgga cctccaatgc agccatttac
    10621 gtcggttacg atcaattcat aatttcctgc acaaagattg cttgcattgg cagttgtttg
    10681 tgaaagtgga tcgttccact gataaaagta aggcgcggtt ccacctgtta cacttgaagt
    10741 tgctattcca ttacaatcac cattacaatc aagcggtgat gcatttaata ccagactaat
    10801 ctgatccggc tcctgaattg ttatctgagg aggaatgaag gtacaaccat tagcgtcaaa
    10861 tacagttggt tgatattgcc ctccgcacaa ggctgttgct tgttgagtgg tttgtgatgc
    10921 gggatcgtcc cacaaaaaag tatacggcga aattcctccg gaaggaaata ctgtgcctgt
    10981 tccattgcac tgtccctgac aatctgcatc ggatgaggct atcgttgctg atagcggtga
    11041 cggttcatct ataattactg atccgttaac tgtgcaaccg gaagcatcgg taacaacaac
    11101 actaaatgtt cccggacaaa gattaaagga ggttgaagtt gtttgtcctg ccgcattcca
    11161 cgcaaaacta tatggcgctg ttccacctgc aggtagagct gttccggttc cggtacaagt
    11221 tccaaaacac aaaacgtcag tgttggacat tgataaagtg agtaaaggcg gttcaattac
    11281 atcaacagtt gcaattgtaa tacaacccaa accgtttgta acagtaaccg cataggttcc
    11341 tgcacataaa cctgtaaccg tatttgttgt tgatgctgcc ggatcattcc aaactaaagt
    11401 acacgttgga tctgcacagg tcacgtttac actaacagtt ccattacaat ctccaaaaca
    11461 ggaaacctgt gtagcagcca catccagaat ttcgttggtg ttatcactta caccaacggt
    11521 tattacctca gtacatccat tagcatctgt gatggttgca tcataaatac ctgcacataa
    11581 tccgcttgcc gtcggagtag tttgtgcgga agcatttacg ctccataaaa tggaaagaat
    11641 acctgtgcct ccggatggat taaccgttgc gcttccgttg catacaccac acgttgcttg
    11701 cacagaagag ctggtggcag taattgctgt tggctctgtt attgtagctg ttgcgtttcc
    11761 ggaacagccg tttgcatcgg taacttgtac gttgtaattc ccatcacaca atcctgttgc
    11821 ggtcagactt gtttgtgcag cgaggtcatt ccatacaata gcaaaaggag cggtgccacc
    11881 aacgatagaa actgttgccg taccattaca aacatcaaag caggtagcat tagtccctga
    11941 agcagttaca gtaagaacag atggctctgt tacagtaaca gctgaaggcg tgaatgaaca
    12001 cccgtttgcg tcagtaataa ttacactgta cgatccaccg caaagtgaga tggcggttat
    12061 tgtgttctgc gaaaaaggat cgctccatga aaatgtataa ggagcagttc cgccggcagg
    12121 tgcaactgtt gcggttccgt tacaaattcc gttacaacta gccggtgttg ctgttgcgct
    12181 tcccgataag gccgttggtt gtgtaacaaa tgaatttaat ggtccggttg tacatccgtt
    12241 tgcatcggtt acaattactg aatatgaacc tgcacataga ttgtttgcaa caggggttgt
    12301 ctgatttcct gctgctgcat tccattggta agaatagggc gctgttcctc ccacaggtgc
    12361 aactgaaaca cttgcattgc aatcaccatt acataataca gtggcgttgg ttgttgtggc
    12421 tgacaataac agtggctcag aaattgaaaa attttgcggt gttgaagcac aaccattggc
    12481 atccgttact gtaactgtat aattcccatc gcaaagtcct gtttctgtaa gtgctgtatt
    12541 tccactcgaa gaccatgttg ccgtatacgg tgctgttccg cccgcaacag taacggttgc
    12601 ctgaccattg cattgattaa aacacaatgc gtttgaactg ttcaattgca caacaatgga
    12661 tgcaggagca acaagtgtga cattgtgtgg tcctgctgaa caccctaaag catctgtaac
    12721 ggtaagcgcg tatgttcctg aacatattcc attcggattc tgtagtgttg atacaatcac
    12781 accagtagca acatcaaccc aagaaaaaga aaaaggagcc gttccaccag aggccgttcc
    12841 agataaggtt ccgttacaaa ctccaaagca agttggattt attccgttgg cagtagcagt
    12901 caattgattt ggttgcgtta gagtaacaac caatggcgca ggggagcaat tatttacatc
    12961 tgtaatggta acataatatg ttcctgcaca caaaccaatt gcatcttctg tggattgtcc
    13021 tattggtaaa ttggtggttt gatcaaacca ttgaaaagta aaaggagctg tacctccgct
    13081 taccgtcaca tcaaccattc cgttacaaaa accaaaacac gatgggctta ctgaaattgc
    13141 acttgcagta ataataacat ttggactaac cgtgacttgt ggtgaattaa cagaacatcc
    13201 attggcatcg gtaatgatgg caaaataatt tcctgcacaa actcctgttg cggttattcc
    13261 ggtttgaccg gttggagtca ttgtagttgc gttcatccac actactgaat atggcgctgt
    13321 acctccaatt atgtttactg ttgttgttcc atcacagatt ccgcaactgg tggttgttga
    13381 ggatgaggca accaccattg taatggggtc tgtaagtgta tagcttgctg ttgctgtaca
    13441 tcccaaacca tcggtaacag tcacagaata attgccatcg cacaatcctg ttgcaggatc
    13501 cgaggtttga ccattcaaaa tatttcccgg ttgctcaaac catgcaatcg tatacggaag
    13561 tgttcctcct gctggacccg cagtagcagt tccatcacaa acacccgcac aagaaggttc
    13621 ggtgaaagag ggatttaatg atagaactgg aggtgctgtt attgtgaatg gatgaatagt
    13681 atcacaacca ttcacgtcta taatggtaac caggaaattt cctgaacata agttgttctc
    13741 tgttaatccc gtattgccgc tgctggacca ggaaacagaa tacggtgaag tttctccgct
    13801 gatgccagat aaaacagctg ttccgttgca ggtaccaaca caggatactg gtgtagtagt
    13861 taatgaaaaa gaataaggag caggttctgc aataacaaat gtagtgtcat aaacacaacc
    13921 attggcatca gtgactcttg cagtataagt tccatcgcat aaaccagtaa ttgaattacc
    13981 ggtaccaagt actgttgcgc cgcttaacca cgaaaccgtg agcgctccgg ttcccccgct
    14041 tgcagaaaca actgaagaag atccattgca atcattatgg caaactgtat ttacaggatt
    14101 taccaataat gcaagtgccg gtgggttggg gatagtcatt gggggaatgg tgttgatcaa
    14161 acaaccattt ccatcttgta cctgggcaaa ataagttccc ggacaaacac cgttaagtgg
    14221 attccctaca ccaattggtg ttgctgtggc agcattgatc caggtaaata catatccggg
    14281 tgttccacca tttgcaatac ctgaaagtgt tgcattacac tcgttatgac atagcggtgg
    14341 cgtaaatgat gttgatgccg taacaggcgt tggctgagtg atggtgaata cagcagaagt
    14401 ctgtgaacaa ccattatcat cagtaactac aacataatag ctcccggcac aaagtcctga
    14461 agcagttgct gaggtttgtc cggcaattgg tgtattgccc ggtgacgtaa accattggta
    14521 agaataattt cctacgcctt gggctgcgtt tacagtaagg ctacctgaac agacattatt
    14581 acaaatcaga ttcgtttgat ttgtgagtgt aacaatgatt ggttgcggct gcgttaccgt
    14641 tgcacttgct gttactgtac aacctgcatt atccgaaact gttactgaat aatttcctgc
    14701 acaaagattg ggattgagag ctgttgttgc gccattcgac catgaatagg aatatggtgc
    14761 tccatttccc cctgttggtt gcgactcaac cgaaccgtta caaacaccaa aacaagccag
    14821 atttgttgtt atgagattgg ggctaatttg tagcggagta agaataaaaa aagtagtgtc
    14881 gaacgtacaa ccgtttgcat ccaccacatt cattgtattt aacccaacac acaattgtgt
    14941 tgcaaccgga gcagtttcac ctgaactcca tgtataagtg tatccgggtg ttccaccaaa
    15001 aacaaacggc gtagccgttc catcacaagt gtaattacaa gaaggatctg ttgttgacca
    15061 tgagagaact gttaaatcac ctggctcaac aatggtaact gtagttgcgc aaatcacatt
    15121 cggtaatact ccggtattaa taaccctaac ggtatatgtt cccgctccga ggccggtcca
    15181 acacgaagtt gcaggtccgc ctcccaacca ttgaatgtta aatggtccgg gtgcacccgt
    15241 aatggtcacg catacttcac catcgtttgc tcctttacaa ctcacatgtt gtccattgta
    15301 atttgtaaca acctgagcat tgattgtaaa ttgattgggg gcagttccac aggtagcagt
    15361 aaggcgcaat tgcgggtttt cggtatcggt gttgcgaatg ataaattgtt ctgtgccatt
    15421 tgcgatccag ttaaccaatg tgcctgcagg atcaatcggc tcgagagtga acaaggatga
    15481 accggacgaa ttaaacacta cctgctgatt ggacgagaag agaatctgat ccgcttttac
    15541 tccttttcca gaagtgaaaa aagcaccaga ctccagcacc agatcaaagc cgggctccag
    15601 ttcgagatcg ttgttctgaa gtgacaggga attataaccc tgtttaatca aattcgctct
    15661 gaagtgaaca tttcctgtct gaagaatcgc atttggctgg gaaggattga agcgaatgtt
    15721 tccgttgata tgattctgaa attgtccggt tacaatccaa tttcctgtaa tggtaaaatt
    15781 gatctttcca tccgactgaa atcggggaaa actgttctgt gcagaaaaat tgaaattccg
    15841 gatagaagca gatacatcaa agcggattac attctttgaa ctcgaaaacg agccggaggt
    15901 gaaaaacaca tcatcattca cacccggtac tccggctccg cctgcaccac catcctgaag
    15961 ggaccaatga gaggcgtccg accaattacc agtgccgcca atccaataat atgctgttgc
    16021 attggaaaac tgaaccaaca acataaaatt caggaaaaac agactttgta aaaatcttct
    16081 catcttctct accggcaagt attcatttca ccattaagac gcagcaacag cagcggtcgt
    16141 tgcccctcta aacggatttt gtaaggttaa ggtaggaaaa ataggataaa cgtcaggtat
    16201 ttttcgctca gttccatgca ggacagtctg taatgccttt tccgcggtgt ggacatgcag
    16261 aaatacccag aataatttcg tgtgtatttg agctggattg ccgaattctt gatgtcgtaa
    16321 aatcaaagga ataacccatt gagaaatgtt tcagaaaatt cagcttaaac aaggcacata
    16381 ttgcatctgt attgcggtag gacaacccga cctgcatttt attttgaaaa tccatcatca
    16441 ggtttagatc gattgccgga gtagacatag gcgcaaactt caaagcagca gagggaatcc
    16501 aggatagatc gtcggaatac ttgaaacgtt ttccgccgga gagaacaaaa tggtgtgtaa
    16561 gacgggattg atcgccaaca tttttccatt tgttgcgcaa agcctgccac atggtgaatc
    16621 caacaaaata atcttcagaa tataaaaaca acccaggggc aatatcgggc cacacaaact
    16681 ggacacttga gccgggtatt gaattatcat ttgggttagc aagcgttact tttcctgcat
    16741 taaatcgcaa ttgctgaaat cctgcataca atcccattcc aagattggtt ttgcgtccaa
    16801 ccggaatatg ataagcgtaa gaaaggtaaa tttttgttct gcttatcgga ccggcaccat
    16861 cgctttccac atgtccgcca attccgtgtt ttgtctttaa atgtttgcgg ttcttgccca
    16921 tgatttgtcc atgtgcgttg gcaaacatgg ttattggtcg gccgtcgaaa ccaacccatt
    16981 gcgttcggta accaacacgc atatccaaac agggcctgct gcctgctact gcgggatggt
    17041 gtgcaaactg attaaaaaca aactgcgtga attgaggtgt ttgttgcgca cttgattgca
    17101 aaaacaatac aacaaaaaca gctcctgtaa atattctctt catcatagca caatggtaat
    17161 tgatcctgaa tactgactgt tctcatcatc cgaccctgta ttcagatcga taacataata
    17221 ataaacaccc gcaggaattt tcattccctt attggttccg tcccatgcat tgttattact
    17281 gtagccctgt ctgttaaaca ccttttgtcc ccaacgatcg taaaccgtta cacgtgcgtt
    17341 ggggaaattc tcgatgtttt gaatttgcca ggtatcattt gttccatctc cgtttggagt
    17401 aaatgtgttt ggaattgaga tgggatcaac cacataaacg acaacctgat cctgatcgaa
    17461 acaaccgtta tctgtaaccg taagtgtata cacagtggtg ttgcccggtg ttgcaaaggt
    17521 cgttgcagaa gtgctgttgc ttaaagaaga agttggtgtc cagttgtagg ttccgccacc
    17581 gctgccggat aaaactgcgg tctgaccagg ggcgatgatc tgatcagcac cggcatttgc
    17641 attgacttca ataatatcca aatcaattgc gttggagctg gctgtgcttg aacatggatc
    17701 tgtgcagctc atgtttaccg aaactgttac atcagatgca aacgttgaag aaattacacc
    17761 tgtggtattg gtagcgaaag gagcaccatt aatcaaccat tggtagtttg cagtacctgt
    17821 acaaccgttt tgtgttgctg taaatgtaac tgcatcatac gaacaggcgg ttgttgtatt
    17881 gcttacaata gaaacagtgg gatcgatttg cggattcaca acaaccgcaa ctgaattgga
    17941 gttagtggaa agtacagcca aacagggatc tgacgaagta acagaacaaa ccacaacatc
    18001 gccattggca actgttcctg atgaaaaact tgttgcagga ccgctttgaa caactgcacc
    18061 attaacagta aatgttaaag caggatttgt tcccgcattt tgaactactg cagtgaatgt
    18121 aacattatct cccgggcatg cgggattggg acttgcggtt aaaactactg aaggcacaag
    18181 attacccggt tgttcaacca gactaattcc acttagcaaa aacgtacatc cgttatcatc
    18241 agtaaccaga acagaatatg ttcctggagc aagattagta aacgcattgg acaattgtgc
    18301 cgctccacca ttgagtgaaa aagtgtaggg cgctgttcca ccggtaaccg ctactgtaaa
    18361 cgatccattt gttgcattgc aatcgggatg aataatagta ggcaaggcat tatccggacc
    18421 tcctgcattg ctgatgactc catttactgt ttgtgtacac ccttgttggt cggacacgat
    18481 aactgaataa tttcctgcag gcaatggatt aaagatatct ccggcttggg gcgggccacc
    18541 atttaaagaa taagtataag gagctgttcc accggtcaca ccggttacct gaaaagttcc
    18601 atcattggtg ttgcatcctg catctattgt tgttgcagtt ccgttttcgg gtccgtttaa
    18661 taaatttaca actatagtgt ccacaaattg aatgcatcct gatgcatcaa tcacccgcaa
    18721 aacataggag cctgcagcga gtcctgtaaa ctgagttccg gattgaaatg cacccccatt
    18781 aagagaatat tggtatgccg gagtacctcc ttgtattgct gtaatgtcta tttcaccatc
    18841 cagattacca caagtctgat cagtaactgc tgaggttgat tgccattcta ctgctgatcc
    18901 gttgacctct attgaaaagg ttccttgagc ggcctgcgtg acccctgcgc cggtgctgtc
    18961 accatcaatg agaatatagt atgtagtatt gggacttaat cctgtaccat tcaattgtat
    19021 tgaagttgca gatcctgcat tgcaatcgat ttctgtatag gttgctgcat tacatgctgt
    19081 acctgccgat aaaagcacag cctgtaattc caggtctgca cctgcccccg gaatacaatt
    19141 aattccagta atggaaacat ttacatcccc tccatttgca ttggttgtaa aactatacca
    19201 aacagaatta ttcaacccga aacatgaaaa ccctgttccg tcttccgggc ctgaatctga
    19261 tccaatattg ctacccgcga atgctgcacc cggacaaagc acctgagcgt tttgacaacc
    19321 attattggcc ggttgggcgt acaagccgat taccggaaaa agaaatacta actgaacaat
    19381 ccacttcatc atgccttttt tcagggtcct aaaggtagcc aattacatct tttttctcct
    19441 atttcccgaa ccccaacacc ctttttttat ctttgaaatg cttttttggt acgattaatg
    19501 caccaagcac catagataat aacccctttg ctatgaacaa catttacaaa acattgaaga
    19561 gcaatcagtt aactcttctc ttttgtttgc tccttgcctt cagcggaact gttcgctcac
    19621 aacacatcaa ctaccaaaac gattcaggtt ggaagatcgg cttcaacatg ggtggtgttt
    19681 ggcaacaaag tgatatgaaa tcaaaggccg gaattggttt cggcgcgaca cttggtaagg
    19741 ccttgtacca aaaacccggt cgctattttg cgttcgactt acggttccgg tacctgcacg
    19801 gatataatta cggatacacc gatacagcga tcactcagtt ccccgcaaca tctatttata
    19861 gcgggaacaa cacagcaaat cctacgttga atgcgtatca gtcgcaaggg aagatgttct
    19921 acaactaccg aatggctctc aacgaatatt cgatggaggg cgttcttacg tttaaccgtt
    19981 tgcgcgaacg tacaggaatt gtgttgtata cctggggtgg aattggctta acaacttacc
    20041 gcgtaaaaac tgaccagatc gataatcctt tcctgggaag cggaactcct tataattata
    20101 ccgctttcga ctctacttca acatggtcgc agctcagcgg aatttgggat ggcgaatatg
    20161 aaacagatgc tgatttaaac cgcggattac aagttggatt tatgccttca tggggtattg
    20221 gtttcggata tcagttctcg ccttattttg caatggttct tgagcataag gtcacttttg
    20281 cgctgaatga taaaattgac ggctatgctt acaaaaatcc tgaaacacct gttaacgatc
    20341 gttaccacta tacaggatta aaattcgtgt tccacttcgg aaaaggaaac ggtcaggttt
    20401 acacaaatta caatacaacc accacaacta caacaactgc tacaacaagc acaagtaccg
    20461 ttgtaacaac tcctccgcgc gcacgaccga ttatcaatat tacccaacca tcaatgagtc
    20521 ctgctaccgt gagcaaccaa tactacacgg ttaatgcaaa catttacaat attgatgggc
    20581 cttccaatat taattttaaa gtgaatggta accaaagcac caacttcact tacaatacat
    20641 caacccaaca attctccagt agcatcctgc tgaacccggg aagtaacgtt gttgagatcc
    20701 gcgctaccaa tgaggtgggg tcagactatc aatcggtggt aatcgtattt gatatgccga
    20761 ttataaacaa catccctcca ccaattgtaa cttttaccaa tcctcccttt actccgtaca
    20821 ataccaacaa ccaatccttt aatgttaccg caactatttt aaatgttgca aactcttcgg
    20881 gaattgcgtt caaagtaaac ggacaaacag taagtggttt ctcttacaat cctacgtccc
    20941 gcgctttttc tgctgttatt ggtcttcatg caggtccgaa cacagtagaa attaaagcaa
    21001 ccaatacttc aggaatggat gtaaaacaag ttgtgatcaa ttaccagccg gttgttgttc
    21061 agcagccacc tgttgttacg atcacaaacc ctacactcaa tccctacacg gtatatgtga
    21121 actctgcgaa tgttgacgca actgtcctca acgttagcaa ttcatcagat attcgcctgc
    21181 gaataaacgg acagctttct ccaaacttca cttacaacac taccacaaaa gtgatgaatg
    21241 tgatcgcgag tctgattccc ggtccgaaca catttgaaat tactgcaacc aatgcagcag
    21301 gtacagattc caaatctact accattgtat atgttgcgcc atcggctctg ccaccagtga
    21361 ttacgtttat cgatcctgct actaatcctg ctactgtaac cacatccacc aaaacagtaa
    21421 gggcaacagt tcaaaatgtt gccggacaaa gcaatattca ggttcgtttg aatggcatta
    21481 ttgttcctaa tttctcatgg aatgcaagta ccaaggttgt acaattcaat gctacactaa
    21541 accccggtgc aaacactgtt cagattacgg ctaccaatac agttggatca gacattaagg
    21601 caacaacaat tatttatcag caggttcaaa ccggacagcc tccgattgtt agcatcagca
    21661 ctccggctca aaatccttac gcaacaacat caccaaacgc cagtattatt ggttcagtgt
    21721 tgaatgtgaa tagtcagagc cagatcacct ttaaggtaaa tggcgttcct tcaaacagct
    21781 tcacctatga tgccgttaca aaagtgttcc ttgccaacgt aacacttaat ccgggaacta
    21841 acgtttatga aataagcgca acaaataact tcggaagcga ttcgcgcaca caaacaataa
    21901 actacacctt gccttgcaac gcaccggtaa ttacattggt acaacctgca agcgcaaact
    21961 tccagacatc attaagcaat ttgccattca ctgcgattgt tcagaatatc ggcaatcagt
    22021 cggctattac atttaagctg aacggtaatg ctcatccttt cacttacaat gcagcaagtg
    22081 gtacagtgac tgcaacactg aatttgagcg ggaccagcaa tactgttgag ttaaaagcga
    22141 ccaacaattg cggaatggct acgcaaagca ttttaatcta ttaccaggcg ccgcttgtac
    22201 caccgatagt taccattaca acgccagcaa caaatccttt cacttctgcc aacgcaagca
    22261 ctgccattac tgctacaata caacatgtaa gttctgcagc aaatgtttca cttaaggtga
    22321 atggaacaag tgtaaccgga tttacattca atccgcaaac tacactgttc agtgcgaatg
    22381 ttaatcttat aagtggtgcc aatacaattg agatcacagg aactaacaat gatggaagcg
    22441 attcgaaaag cacggtgatt atttaccagc ctgtagcacc tccatgtcag caaccggtta
    22501 tagtgctaac tcaaccggca ggaaacaatt attcaacagc attgagttcc ttgcaggtaa
    22561 cagccaatat tctgaatgtg accacaagta ccggaattgt tttccgtcaa aacggaacca
    22621 acgttccctt tacattcaat tctgctacag gagcattctc tgcaaatgtc aatttcacac
    22681 aacctaccaa caacctcgaa attattgcaa cgaacagttg tggcagcagc acaagtacga
    22741 ttagtgtaac ttataatcgt ccacttgtac cgccggtagt taccatcctg aatccggggc
    22801 aaaacccctt caacagtgca gtagcgaaca tgacgcttac cgcaaacatt ctgaacatca
    22861 caggatcatc ggacattact tatacaatca atggtgtatc gagcacaagt ttcacattca
    22921 atccgacaac gaatgttttc accagcaatg tgaccctggc aagcggaaat aatgtattta
    22981 ccatttctgc caccaacgca gatggcagcg actcaaaaag cacaactatt atttacacac
    23041 ctgttgttgt tccttgcaac gcaccggtta tcactccaat tgcaccggct actgcgtcac
    23101 aaactgtgaa tcttgccaat tattcaattc aggcctctgt gttgaatgca acacaaagcc
    23161 agatcacact caaacaaaac ggagtaagca aaacatttac gtataaccca acgacgttct
    23221 tgttaaccgc atccgttaca ctggtagatg gaaccaacaa ctttgaaatc cttgctacca
    23281 acaattgcgg atcagagacg acttcattca gtattgatta caatcgtcca ttggtgccgc
    23341 ctgttgtaac catcactcaa cctgctgcag atccttacac ctcagcaacg caaaacacaa
    23401 ctattactgc aagtattctt cacatcaatg gcagcagcga tatcagcttt aaagtaaatg
    23461 gcgttccaag tacttccttt acgtacaatg ccactactaa agtcttcact tcagctatta
    23521 cccttactcc cggtaataat tctgtagaga tcattgctac aaatacagat ggtactgcaa
    23581 acgactcccg cacgattatc taccaatacg ttgctccttg caaccctccg gccgcaacca
    23641 ttacttcgcc tgctgcaaat acagtagttg ctgttccttt tgcgacgttt actgcaacag
    23701 taacgaatag cagtcaaaac gcaatttctc tgaagcttaa tggcacaacc gttccattta
    23761 cctatacaaa cggaactgta agcgctaatc ttaccttggt agaaggcggc aatgttgttg
    23821 agcttactgc cggaagcaat tgcggtacag cctcggaaag tgtaacgatt acctataatc
    23881 gaccactcac acctccaact gttaccatca gcgctccaca aaacggaagc acaacgcaaa
    23941 catcaacctc tactgtaaat gcaacaatta caaatatcca gaacacttca ggaattgtat
    24001 ttcttgtgaa tggtaatcca agcacctctt tcaacttcaa tgcagccaac aatagcttta
    24061 ctgcatctgt tagtctcaat ccgggagtaa atacttttgt gattaccgca accaatcagg
    24121 atggtcaagc tagtgatcag accagcatta cttaccagcc ttgtatcgct ccgcagatag
    24181 tattgagtca gccttcagca accggaacta ccgtagctaa tgctgcattc aatcttgtgg
    24241 caatggttca aaatggtgcc aacactacaa actcagtcac gcttaatggc gcaaacgtta
    24301 gtgcaacttc aaccggaaac gcaattaact caggacttac attggttgca ggaaccaaca
    24361 cgatcgtcat caatgtagcc ggtccttgcg ggagcgatag tgaaaccatt accattatct
    24421 ataccccatg cttcgatccg gaaatacaac tgaaccagtc gggcgttaac ggtagcacgg
    24481 tgagttcttc agctttcgca ttcagcggta cagttgccaa tgcatcaggc acaagtcttt
    24541 ctgttacttt taatggaagc aacgcaaatg ctacttattc caatggcagc tttaatggca
    24601 acctaactct tgcccaaggg ttgaacacca ttgtaataaa cgtaaccggt ccttgtggca
    24661 ctgactcaaa aaccattcag atcacctaca cgccatgtaa tgctccgctt attaccatca
    24721 atccaaattc aggaggaaac aacgttgcaa acgcggcata tactattaca ggtactgttg
    24781 caaataatac aggcactaca gttgcaatta ctttaaatgg aactgcggtt acctctacct
    24841 attcagcagg atctttttct gccggactca acctgaactc aggcacaaac acaattgtag
    24901 ttactgttac tggtccatgc ggaaacgcta cacaaacagt gaacgtaacc tacactcctt
    24961 gcaacgctcc ggtaattact cttaaccagg ctaatgcaaa tggtacagat caaagtaatg
    25021 cgtcctttgc attatcaggt tctgtaagta accattccgg aacaacagtc agtgttcaac
    25081 tcaatggagc agccgtaaac gcaacctaca acaacggtaa cttcaatgcg tcacttatac
    25141 ttgctgccgg ttccaacact attctggtaa cggtaaatgg tccttgcgga acggatacca
    25201 aaacaatcag ccttacacat actccttgtg ttgcacctgt tattacaatg accagtcctt
    25261 cctctaccgg aattacagta agtaacccaa gctttactgt aatcggttcg gttgctgccg
    25321 gactcaataa tgtaattaag gttaaagtaa acggaaccga agtattcacc acaagtactg
    25381 caaacagtgt aaatacgaac gttacgctga atcagggatc aaacaacatt gagatccttg
    25441 tgaatggcga ttgcggttca gcacaatccg ctttcgctgt aacttatact ccatgtaacg
    25501 cgccggtagt aaatgttgct caatcttctg caaacaattc tacacaaacc gcagctacat
    25561 tttcattaag tggttcggta aataattatc agggaacaac gttaacggtt cagctgaatg
    25621 gcggaacagt aaattccact tataccaatg gtaattatag tgcaggacta acacttgtgc
    25681 ccggctcaaa taatattgtg attactgcca ccggaacatg tggtacagat acaaaaacgc
    25741 ttaccattaa ccatactcca tgtgttactc cattgatcgg aataactaac ccttctgcag
    25801 cgagcacaag ttcaaccact gcttcgttca ctgtttcagg tgctgttacc ggcgggttga
    25861 acaatgtggt tactgtaaaa cataacggaa cagaagtata tcaatccaca acaagcaaca
    25921 atatcaacgt tccgcttaca ttaacacagg gctcaaatac aattttagtt tccgttacag
    25981 gtgattgcgg caatgcatct caaacctata cggtaaatta cactccatgt aatgcccctg
    26041 ttgtaactat tacacaacct gctgcagcgg
//
LOCUS       DAOMYG010000004        23509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_780_length_23509_cov_9.979321, whole genome shotgun sequence.
ACCESSION   DAOMYG010000004 DAOMYG010000000
VERSION     DAOMYG010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 23509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23509
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_780_length_23509_cov_9.979321"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="PK610_00310"
     CDS             complement(<1..1337)
                     /locus_tag="PK610_00310"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRJ34393.1"
                     /translation="MRKFGFSLLCILYFVSCTTKSGQQSDSYSIKTVQSKGKILPNAQ
                     LKKQGRFDSDYLQPKIFNAEHPTVVSAESFIRSLAIKKSVPVKVPQAITPGADTFKLP
                     LRITITDSSFIAGIPESIIAKDAYIKDQNPHNFSSYGKLQGLKHGTINCLLEDQFGNI
                     WIATAGGGVSKFDGKQFSHLTEAEGLCNNNVMCLFQDSRGTIWMGTDGSGITKYDGKH
                     FTTYNDKNGLTSNTVVCINEDQSGNIWIGTNGGGVIKYTADKNKNTGSFEPFTSAEGL
                     AGNHVSSILIDKSGNIWFGTVGGGVSKFNGKQFLNYTIENGLSHNKILCLLEDRQGII
                     WMGTDGSGVCSYDGKTFTHYSENNPLFQEVVWSIYEDKKGQIWLGSWGKGLSKFDGDN
                     FTHYTEKEGLSSNIILSILEDQSGNIWLGTNGGGASVYHGNLFTHFTEKEGLN"
     gene            1580..3553
                     /locus_tag="PK610_00315"
     CDS             1580..3553
                     /locus_tag="PK610_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRJ34394.1"
                     /translation="MRYSSLFLFLGIIFPGISIAQDENHGHCSKLKHFHTQQLRSNTF
                     TISEIAETEEYDVHYYFLDLSMTNTSTYLEGSGEIHAMARVAMDSALFEFFPSFTIAS
                     ILVDGVPVNYSRSNSAIRVPVNKTAGQNMVIHVNYSGTPPTAASNPFGGGGMTNASSP
                     TWGNQVTWSLSEPFSAFEWFPVKQSLKDKADSSQVWITVPYHLKAGSNGILQQVTDLA
                     NGTHRFEWKHRHPIDYYLISVAVAQYIIYDVFANPVNSGQVHIQNFIYDNPATLSNFQ
                     NDIDETADFLELFSVQYGPYPFANEKYGHCMAPIGGGMEHQTMTTQGSFNRTLTAHEL
                     AHQWWGNSVTCSSWSDIWINEGFASYSEYIMLQALYPAQAASNMTGRHNNVMSQPGGS
                     VWVLDSLDAGRIFSGRLTYDKGAAIIHTLRFLIDDDVVFFNGLRSFLTTYANSTASGM
                     DFIAVMENASGKDFTAFMEQWYFGAGYPTYGVLWNNEGDSIHVQISHFTTDPSVTPTF
                     TNDLLLRFDRATLPDTSIRFTITSNQNQFLLPDLQDVVNITAIDPGNWIINQGSGIVK
                     DVNFFVSVAEQTPQPAIRVFPNPADDYVNIEVGNSPNNVLTVFDLQGKVILSTPFGTS
                     LRLDTSGFPSGAFLIRVSSENATIGIQKLIKRN"
     gene            3560..3805
                     /locus_tag="PK610_00320"
     CDS             3560..3805
                     /locus_tag="PK610_00320"
                     /inference="COORDINATES: protein motif:HMM:NF019126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HRJ34395.1"
                     /translation="MEGLPIGTVQLFAFSFELRDWKICDGSLLTIPENRTLFTLIGNR
                     YGGDGIHNFALPDLRKMVPIEGMTYQILVSAYFPAMA"
     gene            complement(3929..4855)
                     /locus_tag="PK610_00325"
     CDS             complement(3929..4855)
                     /locus_tag="PK610_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRJ34396.1"
                     /translation="MTQIDKHSTERFGNLDFLAKQVVEGFITGLHKSPFHGFSVEFAE
                     HRLYNTGESTRHIDWKLFARSDKLFVKRYEEETNLRCQIVIDTSSSMLFPEQTEKGQY
                     NKIGFSIHAAAALIELLRRQRDAFGLSLFAEDIELHTPARSSYTHQKFLFNELEKKLR
                     SFSPTTAHTTRAASSLHRIADAIHRRSLVFVFSDMFDTSEQSEELFSALQHLRHNKHE
                     VILFHVTDKTLELDFKFENRPYTFIDMESGESIKLNPSAVREHYLEAVNRLKKDLVLK
                     CAQYRIDLVEADIRDGFDKVLLEYLIKRSKMI"
     gene            complement(4927..5244)
                     /gene="trxA"
                     /locus_tag="PK610_00330"
     CDS             complement(4927..5244)
                     /gene="trxA"
                     /locus_tag="PK610_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRJ34397.1"
                     /translation="MATEITDANFEEIVMKSDKPVMIDFWAEWCGPCRMIGPVVEEMS
                     KEFEGKALIGKVNVDHNPGISAKFGIRNIPTILFLKNGEIVDKSVGAVPKTQLSEKLN
                     KQL"
     gene            complement(5337..5882)
                     /locus_tag="PK610_00335"
     CDS             complement(5337..5882)
                     /locus_tag="PK610_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34398.1"
                     /translation="MEAIADLLKIMIPALIVMGTAYFLIARQQEQFKQFLDTKTANKL
                     AELKAESNKTTLPLRLQAYERVILYLERISPNNMIMRVYKPGMSSKLLQAELARTIRE
                     EYEHNMTQQVYVSHNCWLLLKNAKEETIRMINVAAAGIPGDASGLELSAAVFEIGQKI
                     DKMPTDIAINFAKNEVQKLFS"
     gene            6097..7920
                     /gene="rpsA"
                     /locus_tag="PK610_00340"
     CDS             6097..7920
                     /gene="rpsA"
                     /locus_tag="PK610_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRJ34399.1"
                     /translation="MAEKSTATAVAPDPNFDWVAYADKGNVYSKAERESVGKMYDDTL
                     TSITEKAVTHGSVVAITKKEVVINIGYKSEGVVPLSEFRYNPNLKIGDAVVVFVESTE
                     DKSGQLIISHKKARMFHAWERVNEALTKDEIITGFVKCRTKGGLIVDVFGLEAFLPGS
                     QIDVKPIRDYDVYVGKNMEFKVVKINQEFKNVVVSHKALIEAELEEQKKQIIGGLEKG
                     QVLEGTVKNITSYGVFVDLGGVDGLIHITDLSWGRVNHPEEIVKLDEKINVVILDFDD
                     DKKRIALGLKQLTSHPWDALDTQLNVGDKVKGKVVVIADYGAFVEIAPGVEGLIHVSE
                     MSWSQHLRSAQDFLKVGDEVEAVVLTLDRDDRKMSLGMKQLKADPWAEIESKYPTGSR
                     HNALVRNFTNFGVFVELEEGIDGLIHISDLSWSKKVKHPSEFCKVGDKMDVVVLELDK
                     ENRRLSLGHKQLEENPWDVFEGIFTEGSIHEGTVVSVTDKSATVSLPYGVEGYAPSKH
                     FRKEDGSNAKGDEKLPFMVLEFNKEGKKIVVSHTATFDASVIEEKDRKAAARGGKKTT
                     AATGGTSKAVKEINSNIEKTTLGDLDALANLKADLEKGEGK"
     gene            8012..8419
                     /locus_tag="PK610_00345"
     CDS             8012..8419
                     /locus_tag="PK610_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34400.1"
                     /translation="MEKKIRSQRIEKGIVENYVLEDMHIEPEDLDLCKSDNLALMGDE
                     LYTVLVVSGSGSTISAEARKKVASPEYRMNTVAKAFLVENLAHRIIGNFYMTFNKPAI
                     PTRLFTDREKALEWLRQELKINTEKAVGTEMKN"
     gene            8458..9006
                     /gene="pyrR"
                     /locus_tag="PK610_00350"
     CDS             8458..9006
                     /gene="pyrR"
                     /locus_tag="PK610_00350"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRJ34401.1"
                     /translation="MKPVTIVNTQLFSLLINRLCHQLIENHKDFSESAIIGLQPRGIY
                     LANRLKQRLEEITGKTIQTGALDITFYRDDFRRRDVPLLPSVTNLDFSIENKDVILVD
                     DVLYTGRTVRSGLDALIAYGRPRKVELLALIDRRFKRQIPIQADYVGKWVDTLSSEKV
                     AVEWRETEGEDKVILFTPKEHE"
     gene            8999..9931
                     /locus_tag="PK610_00355"
     CDS             8999..9931
                     /locus_tag="PK610_00355"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRJ34402.1"
                     /translation="MNKNLSVDHLLGIKDLNREDLEIIFKTADSFKEVINRPIKKVPS
                     LRDITIANLFFENSTRTKLSFELAEKRLSADVVNFSASGSSVKKGETLIDTVNNILAM
                     KVDMVVMRHSTPGAALFLSKHVDARIINAGDGTHEHPTQALLDAYSIRERLGSVEGKK
                     VVIVGDILHSRVALSNIFCLRKLGAEVMVCGPTTLIPRYISELGVKVEHNLKKALEWC
                     DVANMLRIQLERQDIRYFPTLREYTMQFGLDMKLLKSLNKEIVVMHPGPINRGVEITS
                     EVADSHQSVILDQVENGVAVRMAVIYLLASKIRS"
     gene            10027..10410
                     /locus_tag="PK610_00360"
     CDS             10027..10410
                     /locus_tag="PK610_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRJ34403.1"
                     /translation="MNFEVFPHEKYVLIRSKVEKLDTGHAPELKSLVVFHNKEGVRNI
                     IIDLSDTRYCDSSGLSSILVANRLCRSSNGSFVLTGLQEPVMKLITISQLHTVISITP
                     TANEASDLVLMEEVERELTSDDENQ"
     gene            10427..11182
                     /locus_tag="PK610_00365"
     CDS             10427..11182
                     /locus_tag="PK610_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ34404.1"
                     /translation="MKKLLLFIALGLSASVASAQCTPDMSVAGDAGIHPDSATNFMPA
                     YVGTPYNQVVTAVVPADTCAQILPLPFPCTTLSFDYIEVTSVTGLPTGFTFTCAPANC
                     QFMGNTIDCAIISGTAQPGQEGVYPLVFNLDAYVGGLGVPNSFTLTYYRIVVNPAVGT
                     IELSQDAFAVSQNQPNPFDESTLIELETSVSGLVQFEVFNLIGQPVHTRTVSVQKGKN
                     QIRFEAGDLPAGTYMYKVSDGKRSTTKRMVVTR"
     gene            11236..12156
                     /locus_tag="PK610_00370"
     CDS             11236..12156
                     /locus_tag="PK610_00370"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HRJ34405.1"
                     /translation="MKFEVTILGSGSSTPSLRRNPASQLVNVQENYFLVDCGEGTQLQ
                     MRRFKIKFQRIDHIFISHLHGDHYLGLIGLLQSMHLLGRKKEVHIYGHPDLKTMIDLH
                     MKISSTRLTYPLEFHALNYDAPEIIYSNKVVEVSTVILRHRIPTCGFIFREKPKLLPL
                     SSEKIRKHKIPVFALPAIKEGADYTTENGTVIPNHELTLPRPPSHSYAYCSDTIFTEK
                     IVESIQGVDVLFHESTFTKELEDRAKETYHSTAAQAATIAQKAKVDRLVLGHFSVRYN
                     DLEPFLEEAREHFKNVQLATDGITINVYPQ"
     gene            complement(12157..13107)
                     /gene="fmt"
                     /locus_tag="PK610_00375"
     CDS             complement(12157..13107)
                     /gene="fmt"
                     /locus_tag="PK610_00375"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRJ34406.1"
                     /translation="MKIVYMGTPEFAVAGLDALRKAGNEICAVVTATDKPAGRGQKLS
                     PSAVKVYATENNIPVLQPEKLRDPEFLNTLKNYKADLFVVVAFRMLPDVVWKMPIKGT
                     INLHASLLPNYRGAAPINWAVINGEKETGVSTFFIREEIDTGNVIAQRKVSIGENETA
                     GELHDKLMLVGAELLVETVKAIESGTAHSIPQEEMSVGTKNFPVATKIFREDCKIDWT
                     KKTDSIFNLIRGMSPYPAAFTTLVQSGKEETLVKIFASRKGNSTGKPGSIHTDGKTFL
                     EVVTNDGSLLLTDIQQAGKKRMGIEEFLRGTKLSEDARML"
     gene            complement(13113..14453)
                     /locus_tag="PK610_00380"
     CDS             complement(13113..14453)
                     /locus_tag="PK610_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRJ34407.1"
                     /translation="MNQLNAPLSYGRILAVASPIMVGVFVQFTMLITDSAFLNRIGSL
                     DFNASGNAGMLYVTLTMFAMGISNGAQVLIARRDGELKLKEAGNVFRQSLLILCGLSV
                     LFTILLLVGSHKFLPAIVKDAETGIKMQEFLDIRSWGFFFALPAMAFQGFYTGVARTR
                     IIMYSTVITALLNILLDYLLIFGNYGFPELGLQGAAIATVIAEAAALLFVVIYMLIDK
                     ESKPYSPFGNMRFNTSDSLELMRLSFPLMLQGIVSMGTWTIFFFFIEQLGPANLEVSQ
                     VIRIFYLIALIPVLGLGSATRTFVSHLIAEGKTNEVVPTVKRIIFLNLIATVLFTFPN
                     LFFPHLAVPFISTNPEILDTTANTLQIVTGAMFLLAASSPLNALIAGAGATQTAFRIE
                     LFSIAIYLFGAWLLTVKFPQPIVYVWCLEYVYFLLMFFACLVYIRKGKWKDIKI"
     gene            complement(14450..15622)
                     /locus_tag="PK610_00385"
     CDS             complement(14450..15622)
                     /locus_tag="PK610_00385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRJ34408.1"
                     /translation="MTNSIIQLKPGKEKSLERFHPWVFSGAIHRIDGQPEEGEMVMVC
                     DRSGKTLGYGHFQTGSISIRMLQFGETRPDGGFWKAKIAAAWNMRNAVCPENTNVFRL
                     IHGEGDGLPGLVIDYYAGYCIVQCHSIGMYKQRSEIVEALKAVVGNSLKGIYDKSTET
                     LPKTHTEGTSLSLYGDAAGSTEVMEYGHRFVVDWESGQKTGFFIDQRENRKLLGSYAK
                     GKKILNTFCYSGGFSVYALNEGAREVHSLDSSKKAIELTDKNIALTAHADKHKSIVAD
                     AIPYIRNIENDYDIIVLDPPAFAKHLSAKHNAIQGYRRLNEEAIRQIKPGGLLFTFSC
                     SQVISMELFTGAVFSASANAKRNVRILHRLHQPADHPVSIFHPEGEYLKGLVLYIE"
     gene            15700..16335
                     /locus_tag="PK610_00390"
     CDS             15700..16335
                     /locus_tag="PK610_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34409.1"
                     /translation="MKLLAIRVLPFLALFFFSCGSESPVPDEIVSDTLISPEYADTTV
                     SENENTETLSSRPDPEELLESFIYCTESAENSMTCKYFIAKAICMYYGINDFESDEGT
                     YINFEEILPRIKSSDKWYRAGDSFNQDVLVKAQDAVNNGKAALAISEQEQYGHVVLIL
                     PGKTEAAPAWKNLSVPQVASFFMVPGIQPFVNRSMAFAWRAPEGIGIYIRK"
     gene            16341..16838
                     /locus_tag="PK610_00395"
     CDS             16341..16838
                     /locus_tag="PK610_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRJ34410.1"
                     /translation="MIRTEQEVLADLLNYNRKSTLGYLSKIKEFDLTKTFSAEGKELN
                     SPIWILGHLATTQNYLCLKSTGAEMVRFSWAKLFGMGSVIPDAAGLPPVEEIVNVLNE
                     VHQRSLTRILSLSNEDLALPTTTGFAMGGEDSYRAIIRHAIMHEGIHAGHMGWICKLW
                     GVKTI"
     gene            complement(16902..23186)
                     /locus_tag="PK610_00400"
     CDS             complement(16902..23186)
                     /locus_tag="PK610_00400"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HRJ34411.1"
                     /translation="MIKPNSLISNTLKTITLICLFVFLSSSSKAQITITPGGSSATII
                     NSLVGQGITVSNVVINCGGTAYGTWSGNLGAGGVGMTNGGIILTTGSAAAADGPNNSG
                     SAGATVAGFDFSDPQLTTQPGAGSPAPSFDNCILEFDMIPTCSQFNVAFVFGSEEYPE
                     FVSSSFNDGFGMFITGPNPLGGNYTNFNFARLPNNQLVSIDNVNATTNAAFYNTNTTG
                     IMQYDGYTDGLVATINVTPCQSYHIKIIIADAGDQIYDSGLFLGYQSFSCTTPPLTLT
                     PSMTPATCGASNGSAGVTLSGGTGPFSILWSPGGQTTNTISNLAPGNYTVTVTDPLSC
                     TPPITQTITVTSTGAAPILNPVSTNATCGCNGSINLNTSGGVAPFTFAWTGGLATGNP
                     TNVCPGNYAVTVTGANGCTATASATVTGPAPLTATTAATHVLCNGGNNGSATVTPAGG
                     TPPYTYSWSPGGQTGQTASNLTAGTYTVTVTAQGGCTVTSSVTVNQPAALTGTLTPTN
                     VTCFGLNNGQISVNASGGTGTLQYSINGGAFQAGNSFTGLAPGNYTIIIRDANNCQIT
                     LNTTITQPTQVVGTITATVSSTCGQANGSVTIVGSGGTAPYNYSLNGGPFVGSGVFTN
                     LLPGSYTIVVRDANNCTVSTPVNITINALLTPIASVTNQTNVSCAGGVNGSALIGITG
                     GTAPFSYDLNPAVGPNPPPQASNSFTALAAGNYTVIVTDANNCAATTTFTITQPTQLA
                     FSSVPVHVTCFGQCNGQITVNASNATPPYEYSSNGGATYQASNVLTNLCAGTINVVVR
                     DANGCQVNANVVITQPTQVAATFTGTNPLCQGVCNGQVTASASSGGTAPYQYSVNGGA
                     FQSGTTFTGLCSGVQNMIIRDANGCQVTSQVVLIDPPGYAVNTISSIESNCGFNNGSF
                     QVQASGGLAPYSYNNLTIGIANGTGLFDQLVAGAYEVLVTDANNCQQIAFVGVNDVEM
                     SGVLNSIVDVSCPGLCDGEVYTSATGGFGTISFDLDNGSQSAFGSGDFFNLCPGSHAV
                     TMTDQGLCVYVVSFNVIGPPQIIFTNSSTNVSCFGGSNGTITIDPPIGGTPPFQFSVD
                     GGATYQATGNFTGLAAGTYNLMVRDQNGCTESATATVNQPTQIVVSESHTDLTCNGNN
                     SGTLSIGAVGGTPPYQFSNNNCTTSQAFSSFFGLAANTYTLCVTDANNCVITGTATIT
                     EPAALTATVTPDNPDCFNGADGSMTINASGGTPVYQYSENGGTTFQISNTINGLTAGT
                     YNVQVKDQNGCLFNTTQIIGQPTQVTFTSTPVNTTCGQANGSITINANGGTGAFQFSI
                     DNGTTNQAGNAFNGLAENTYFVVVTDVNGCTATETITLIDASSPVISGVIGTNPLCFG
                     STDGLAQVLVNGGTAPLTFSMDGGPGQASPNFSGLGAGNHTASVVDLNGCTSTFVFTM
                     IEPAVLTLTATPTPLLCFNDFTGGILMSATGGTSPYQYSFDGGVTFGASPSLTFIAGG
                     NYNLSIVDNNGCTVTGSTTVNEPTQLAFQSFTVVDATCFDVCDGQVQAFPTGGTVAGL
                     YHFNWSNSLAGPSQATATNICDGSYSLTVTDDNNCLISQNFTINEPPQVVITSTVITN
                     VTCNGACDATITVNSPLAVSYSTNGGASYQPANNFTNVCAGVYNIIAQDATGCRADTT
                     VTVLEPSVLDLFPPTGGFACSDQPFNLIALGIGGTPPFTYTWSGTTETSSSVNVTSST
                     TTTVTVFATDANGCISPTESATIDVYPLYTATISPDVVICPGTPTTLSGQGSQGLAPY
                     YYQWIGGDTSQTTTVSPSVNTTYQLLTMDICMDTVMLQVNVTMHQLPQVNFAPTVAAG
                     CAPFTTTLVNSTPVGQIGGNCLWDLGVLGTQSNCASITQTFPDPGCYDINLTITSPEG
                     CINDTTIVDAVCVYPNPVAEFSYIPMQPTVLAPNVNFVNESTGATTYLWDFAGIGSST
                     DENPAFVFPSNAPNTYVVCLTAVSQFGCIDSTCHIVTVYDELLVYVPNAFTPDGDGIN
                     DIFLPIVNGADPDPNRYEFMIFNRWGELIYEGHNPTVGWDGKHRGNDSKQDVYVWKIK
                     LQDALTGQQKVYYGHVSLLR"
BASE COUNT         6549 a         4933 c         5193 g         6834 t
ORIGIN      
        1 ttgttcaatc cttccttttc ggtaaaatga gtaaataaat tgccatggta tacagacgct
       61 ccgccaccat ttgttcccaa ccaaatgttt cccgattgat cttcaaggat agaaaggatg
      121 atgttactgc ttaagccttc cttttctgta tagtgggtaa agttatcacc gtcaaattta
      181 gacaaaccct taccccagga acccaaccag atttgtccct ttttatcttc ataaatcgac
      241 cagaccactt cctgaaaaag aggattgttt tccgaataat gagtgaatgt ttttccatcg
      301 taactacaaa ccccgctgcc atccgttccc atccaaataa ttccctgccg gtcttcgagt
      361 aaacacaaga ttttgttatg gctcaatcca ttttcaatcg tataatttaa aaattgtttt
      421 ccattgaatt ttgaaacacc tcctccaaca gtaccaaacc aaatgtttcc tgatttgtca
      481 attaaaatgc tgcttacatg atttccggca agaccttctg cactggtgaa gggttcaaaa
      541 cttcctgtat tcttattttt atccgccgta tattttataa caccaccacc gtttgttcca
      601 atccagatat ttcctgattg gtcttcatta atgcaaacaa ccgtgttcga tgtaagtccg
      661 ttcttatcgt tgtaagttgt aaagtgtttc ccatcgtact ttgttatccc ggatccatct
      721 gtacccatcc agatagttcc ccggctgtcc tgaaataaac acattacgtt gttattgcat
      781 aaaccctctg cttcagttag gtgagaaaat tgttttccgt caaatttgga gactcctcca
      841 ccggctgttg cgatccagat gtttccaaat tggtcttcca gcaaacaatt gatcgttccg
      901 tgttttaatc cttgcaattt gccataggaa ctaaaattgt gagggttctg atccttgatg
      961 taggcatcct ttgcaataat cgattcagga attcctgcaa tgaacgaact gtcggtaatt
     1021 gtaattctaa ggggcagctt aaaggtatca gctcccggag tgatcgcttg tggtaccttt
     1081 accgggactg attttttaat tgccaaagaa cggataaaac tttcagctga aaccacggtt
     1141 ggatgttcag cattgaaaat ttttggttgg agatagtcag aatcaaaacg gccttgtttt
     1201 ttcagttgag catttggaag aatttttcct tttgactgaa cagttttgat agaatagcta
     1261 tcggattgtt gaccgctttt tgtggtgcag gaaacgaaat agagtataca taaaagagag
     1321 aaaccgaatt tcctcattgg ttcgtatcgt tattgccagc tttcggcggt gggtgcataa
     1381 aaatatagaa aaattgaatt agataattgg tttagaggtt tttgttttgg gaccattcag
     1441 gctcctttaa aatctcagga acagcttaat ctgatgttca cttgccgggt accgtgttat
     1501 tgcgattttg accttttcct tactcgatgc aaaaacggct tgaaattatg ttacattgac
     1561 agacaaattg ttttgccaaa tgcgctatag ctcacttttt ttgtttctgg ggattatttt
     1621 ccccggaatt agtattgccc aggatgaaaa tcacggacac tgttccaaac tgaagcactt
     1681 ccacacgcaa caattgcgtt ccaatacctt taccatttct gaaattgcag agacggaaga
     1741 atacgatgtc cattattatt ttctggacct cagtatgacc aacacctcaa cttatctcga
     1801 aggttcaggt gaaattcatg ccatggcaag agtagcgatg gattctgcct tgtttgagtt
     1861 ttttccttcg ttcacgattg cttccattct tgttgatggt gtgccggtaa attattcgcg
     1921 cagcaattct gctattcgtg ttccggtaaa caaaacagcc ggtcagaata tggtaattca
     1981 tgtcaactac agtggcacac ccccaactgc tgcatccaat ccttttggtg gcggtggtat
     2041 gaccaatgcc agttctccca cctggggcaa tcaggttacc tggtcgcttt ccgaaccttt
     2101 ttctgctttc gaatggtttc cggtaaagca atcactcaag gataaagccg atagttctca
     2161 ggtgtggatc acagtcccct atcacctgaa agccggctcc aatggaattt tgcagcaggt
     2221 aacagatctt gccaacggaa cacatcgttt cgagtggaaa caccgtcacc ccatcgatta
     2281 ttacctgatt tcagttgcag ttgcgcagta tatcatttac gatgtatttg caaatcctgt
     2341 taattccggt caggttcata ttcagaattt catttatgat aatcctgcta cgctgagtaa
     2401 ttttcagaac gatattgatg aaacggctga ttttctggaa ctgttttctg tgcaatacgg
     2461 accataccct tttgccaatg aaaagtatgg tcactgcatg gctcccatag gcggcggaat
     2521 ggaacaccag accatgacca cccagggatc gtttaaccgt acactcacgg cacatgaatt
     2581 ggcgcaccaa tggtggggaa attccgtaac ctgttcttcc tggtcggata tctggatcaa
     2641 tgaaggattt gcttcgtatt cggaatacat catgttgcag gctttgtatc cggcacaggc
     2701 agcatccaat atgaccggac gacacaacaa tgtcatgtcg caacccggtg gtagtgtttg
     2761 ggttttggat tcattggatg caggaagaat tttttcagga cgcttaacct acgataaggg
     2821 cgcagccatc attcacacct tgcgtttttt gatcgatgat gatgtggttt tcttcaatgg
     2881 tcttcgtagt tttttaacca catatgccaa tagcactgcg agcggaatgg attttattgc
     2941 ggtgatggaa aatgcttccg gaaaagattt caccgcattc atggaacagt ggtattttgg
     3001 agctggatat ccaacctacg gagtgctttg gaacaatgaa ggcgattcta tccatgttca
     3061 gatcagtcat tttacaactg atccttctgt tacaccaaca tttaccaatg acctcttatt
     3121 gcgttttgat cgtgccactt tgcctgatac ttctattcgg tttacgatca cctccaatca
     3181 aaatcaattt cttcttccgg atttgcagga tgtggtcaat atcaccgcca tcgatcccgg
     3241 taactggatc atcaatcagg gttcgggaat tgtgaaagat gtaaactttt ttgtgtcggt
     3301 tgcagaacaa acacctcagc ctgcgatccg tgtttttcca aatcctgcag atgattatgt
     3361 gaatattgaa gtgggaaata gtccgaacaa tgtgcttact gttttcgatc ttcagggaaa
     3421 agtgatactg agtacaccat ttggtacttc attgcgactg gatacttccg gttttccgtc
     3481 cggcgcattt ttgattcgtg ttagctctga aaatgctaca atcggaattc aaaaactgat
     3541 taaacgaaac tagaaattta tggaaggatt accgatcgga actgttcagt tgtttgcttt
     3601 ttcattcgag ctacgtgatt ggaaaatttg tgacggatca ctattgacca tccctgaaaa
     3661 ccggaccctg tttaccttga ttggaaacag gtatggagga gatgggattc ataattttgc
     3721 tttgcccgat ttgagaaaaa tggtgcccat cgagggaatg acctatcaga ttttggtatc
     3781 tgcttatttc cctgcaatgg cttgagaata aaaactgtac aacgcaagcc gttccggtta
     3841 agaaagaaca tacccagatt acaatggctt taattgaaaa tccggtaaaa attcttcttt
     3901 taagattgcg aataagggag gttctctgtt aaatcatttt cgatcgcttg atcaggtatt
     3961 ccagcaatac tttatcaaag ccatcacgaa tatcagcttc aacgaggtcg attcggtatt
     4021 gcgcgcattt tagaacgaga tcctttttta aacggttcac tgcttcgagg taatgctcgc
     4081 gtacagcaga tggattcagt tttatgcttt caccgctttc catatcgata aaggtgtacg
     4141 gacgattttc aaatttaaaa tcgagctcta gtgttttatc ggttacatga aagagaatga
     4201 cttcatgttt gttatgtcgc agatgttgca aggcagaaaa taattcttcg ctttgttctg
     4261 aggtgtcgaa catatcgctg aatacaaaaa caagcgaacg gcgatgtatg gcatccgcaa
     4321 tacgatgaag cgatgaagcc gcgcgtgtgg tatgagcagt agtaggggag aaggatctta
     4381 gttttttctc cagttcatta aacaggaatt tctgatgcgt atagctactg cgtgccggtg
     4441 tgtgcagttc gatgtcttct gcaaagagac ttaatccaaa tgcatcgcgt tgtctgcgca
     4501 aaagttcaat caatgcagca gcggcatgaa tggagaatcc gatcttattg tattgccctt
     4561 tttcggtctg ctccggaaaa agcatcgagg atgaagtgtc gatcacgatc tgacaacgta
     4621 aattggtttc ttcctcgtac cgctttacaa ataatttatc cgatcgggca aataatttcc
     4681 agtcgatgtg ccgggtggat tctccggtat tgtacaaacg atgttctgca aattcaactg
     4741 aaaatccatg gaacggactt ttgtgcagac cggtaataaa accttccacc acctgtttgg
     4801 ccaggaaatc cagattaccg aatcgctcgg tgctatgttt gtcgatctgc gtcacactgc
     4861 aaaataagaa ttccggacca aaggcccgga attcattgaa acaactttac tcaggaggga
     4921 ggtagcttaa agttgtttgt taagcttttc agacagttgc gtcttgggaa cagcacctac
     4981 tgatttgtct acgatctcgc cattcttaag gaacaggata gtaggaatat tgcgaatacc
     5041 gaattttgca gagatgccgg gattgtggtc cacattcact tttccgatga gggcttttcc
     5101 ttcaaattct ttcgacattt cctctactac cggaccaatc atacggcaag ggccgcacca
     5161 ttctgcccag aagtcgatca ttacgggttt gtcggatttc atcacgatct cttcaaagtt
     5221 cgcgtcggta atttcagttg ccattgttat gtttttttga ggttataaaa gtacactttc
     5281 agaataaacg ctaaaacccg gccattgttg ataagtggac agattgtcag aatttattaa
     5341 ctgaaaagct tctggacttc gtttttggca aagttgatcg ctatgtcggt tggcattttg
     5401 tcgatctttt gtccgatttc aaacacagca gcactcagct caagtccgct tgcatctccc
     5461 ggaattcctg cagcagccac attgatcatg cgtatggttt cttccttggc atttttgagt
     5521 aaaagccagc agttgtgcga tacatagacc tgttgtgtca tgttgtgctc gtattcctca
     5581 cggatggtcc ttgccagttc cgcctgcagt agttttgagc tcataccggg cttgtacacc
     5641 ctcataatca tgttgttggg cgaaatgcgt tcgaggtaca aaatcaccct ttcgtaagcc
     5701 tgaagtcgga gcggaagcgt ggttttgttg ctttctgctt ttagttctgc cagtttattg
     5761 gctgtttttg tatcgagaaa ttgcttgaat tgttcttgct gcctggcaat aagaaaatag
     5821 gctgtaccca ttacaatgag tgccggaatc atgattttca ggaggtctgc aatagcttcc
     5881 atgctgctgg tttttctcta caaagataag gaaaagcttg cccaaagggg aatacaccgg
     5941 aaggcttcct ctggctgcag ccgaaatcct tcatttttca ttacagattc ccattttttt
     6001 actacttttg cagccttcat aagaagatgc ctatccgact aggtatttct aacttcttcc
     6061 atcggtcgga cgatgggata caagataata agccaaatgg cagaaaaatc taccgcaaca
     6121 gccgttgcac cggaccctaa ctttgattgg gttgcttacg cagacaaagg gaatgtttat
     6181 tcaaaagccg agcgcgaaag cgttgggaag atgtatgatg ataccctgac atctatcact
     6241 gaaaaagccg ttacccacgg ttcagtggtt gctattacca aaaaggaagt agtgatcaac
     6301 atcgggtata aatcagaagg cgttgttcct ctttctgaat tccgttacaa tcctaacctg
     6361 aaaattggtg atgcagtggt tgtattcgtg gagtcgacgg aagacaaaag cggtcagctg
     6421 atcatttcgc acaagaaagc acgtatgttc catgcatggg agcgtgttaa cgaagcgctt
     6481 accaaagacg aaatcattac aggttttgtt aagtgtcgta ccaaaggtgg acttattgtt
     6541 gacgtattcg gattggaagc attccttcca ggttcgcaga tcgacgttaa gcctattcgt
     6601 gattacgatg tttacgttgg taaaaacatg gaattcaaag ttgttaagat caaccaggaa
     6661 ttcaagaacg ttgttgtttc tcacaaagcg cttatcgagg cagaacttga agagcagaag
     6721 aaacaaatca tcggcggact tgaaaaaggt caggtactcg aaggaactgt taagaacatt
     6781 acttcttacg gtgtattcgt tgaccttggc ggtgttgatg gtcttatcca tatcacagac
     6841 cttagctggg gtcgcgttaa tcaccctgaa gaaatcgtta aactcgacga gaagatcaat
     6901 gtggttatcc tcgatttcga cgacgacaag aagcgtattg cacttggtct gaagcagctt
     6961 acttctcatc cttgggatgc actcgacacg cagcttaatg ttggtgataa agtgaaaggt
     7021 aaagttgttg ttattgcaga ctacggagca tttgtagaaa ttgcacccgg agttgaaggt
     7081 ctgatccacg tttcagaaat gagctggagt caacacctgc gctctgcaca ggatttcctc
     7141 aaagtaggag acgaagtgga agctgttgta cttaccctcg accgcgatga tcgcaaaatg
     7201 tcgcttggta tgaagcaact caaggctgat ccatgggcag aaattgaaag caaatatcct
     7261 acaggttcac gtcacaacgc actcgttcgc aacttcacca actttggtgt gtttgttgaa
     7321 cttgaagaag gtatcgacgg actcattcac atcagcgatc ttagctggag caagaaggta
     7381 aaacatcctt ctgaattctg taaggttgga gacaaaatgg atgtggtagt tcttgaactt
     7441 gataaagaaa accgtcgttt gagtcttggt cacaaacaac tcgaagagaa tccatgggat
     7501 gtttttgaag gcatctttac agaaggttca attcatgaag gaactgttgt tagtgtaacc
     7561 gataagtcag ctacagtttc tcttccctat ggagtagaag ggtatgcacc ttccaagcat
     7621 ttccgtaaag aagacggatc gaatgctaaa ggcgatgaaa aacttccatt tatggttctt
     7681 gaattcaaca aagaaggcaa gaagattgtg gtgtcacaca ctgcaacatt cgatgcatct
     7741 gttattgaag aaaaagaccg taaggctgct gcacgtggtg gtaagaaaac tactgctgca
     7801 acaggtggaa cttcaaaagc tgttaaggaa atcaattcaa acatcgagaa aaccactctt
     7861 ggtgatcttg atgcgcttgc taaccttaag gctgacctcg aaaaaggtga aggcaaataa
     7921 ttctgatcca ttgatcataa taacaagggc ttccgtcagg aagcccttgt tattttacct
     7981 attttttacc taattgccgc tgcattataa aatggaaaaa aagatccggt cacaacgcat
     8041 tgaaaaaggt atcgtcgaga attatgttct ggaggatatg catattgaac cggaagatct
     8101 tgatttgtgc aaaagcgata acctggcctt aatgggcgat gagctctaca cggtactggt
     8161 tgtttccgga agtggaagca ccatctccgc cgaagctcgt aaaaaggtgg cctccccgga
     8221 ataccgcatg aatacggtgg caaaagcatt ccttgttgag aacctggctc accggataat
     8281 cggcaatttt tacatgacat tcaacaagcc cgcaatccca acacgcctgt tcaccgatcg
     8341 tgaaaaagca ttggaatggt tgcggcagga actcaaaatc aatacagaaa aggcggtcgg
     8401 caccgaaatg aagaactaaa atcatttttt tgtttacctt tgactgacct tccaacaatg
     8461 aagccggtaa ccatagtcaa tactcagctc ttctctctcc tgatcaaccg gctttgtcat
     8521 caacttatcg aaaatcacaa ggacttttct gaatccgcca ttatcggact tcagccgcgt
     8581 ggcatttatc ttgcaaatcg cctcaagcaa cgtctggaag aaattaccgg taaaacaatt
     8641 caaacaggcg ctctcgatat taccttttac cgcgacgatt tccgacgcag agatgtaccc
     8701 ttgttgccca gcgttaccaa tctcgatttc tccatcgaaa acaaagatgt aattctggtc
     8761 gatgatgtgt tgtacacagg cagaacagtg cgttcaggtt tggatgcact cattgcatac
     8821 ggccgaccaa gaaaggtgga attacttgcc cttatcgatc gccgctttaa acgtcagatt
     8881 cccattcagg cagattatgt aggaaaatgg gtggataccc tttcctccga aaaagttgca
     8941 gttgaatggc gggaaactga aggagaagac aaagtgatcc tttttactcc gaaagaacat
     9001 gaataagaac ctcagtgtag accatctgct cggaattaag gacctcaacc gtgaggacct
     9061 tgagatcatt tttaaaacag ccgatagttt caaggaggta attaacagac cgataaaaaa
     9121 agtcccctca ttaagggata ttaccattgc caatttattt tttgaaaact ctacccgtac
     9181 taagctctct tttgagttag cagaaaaacg actatctgcc gatgtggtga atttttcggc
     9241 atccggatca tccgtgaaaa aaggggagac actcatcgat accgtcaaca acattctcgc
     9301 aatgaaagta gacatggtag tgatgcgtca ttccactccc ggagcagcct tgtttttatc
     9361 gaaacatgtg gatgcacgaa ttatcaatgc cggcgatgga acgcacgaac atcctaccca
     9421 ggcgctgctg gatgcgtatt cgatacggga aagattggga agtgtggaag ggaaaaaagt
     9481 ggtgattgtg ggagatattc ttcattcaag agtcgccctt tcaaatattt tctgcctcag
     9541 gaaacttggg gctgaagtaa tggtttgcgg accaacgact ttaattcccc gctatatttc
     9601 tgagctgggt gttaaagtgg aacataacct gaaaaaggcg ctcgaatggt gcgatgttgc
     9661 caacatgtta cgcattcagt tggaacgtca ggatatccgg tacttcccga cattgcgcga
     9721 atatactatg cagtttggtc ttgatatgaa gctgttaaag agcctgaaca aggaaattgt
     9781 ggtgatgcac ccaggcccca tcaatcgcgg tgtggaaatt accagtgagg ttgccgatag
     9841 tcatcagtcg gttattcttg accaggttga gaacggggta gcggttcgaa tggcagttat
     9901 ttacctgctg gcctccaaaa tacgctcctg agtatttacc aacaagcccg gttgaaaaga
     9961 cagtcctttt ccggttttct tacgatttaa aaattcttac atttaggctt atcaaatttc
    10021 caggacatga attttgaagt ctttccacac gaaaaatatg tgcttatccg cagcaaggtg
    10081 gagaagctgg ataccggtca tgccccggaa ttgaaatcgc tggttgtttt tcacaataaa
    10141 gaaggcgttc gcaatatcat cattgatttg agtgatactc gctattgcga ttcatcagga
    10201 ctcagttcca tactggttgc aaacagactt tgtcgttctt ccaacgggag ttttgtgttg
    10261 accggacttc aggaacctgt gatgaaactg attaccattt ctcaattgca tacagttatc
    10321 tctataactc caacggcaaa tgaagcctca gacctcgttc tgatggaaga agttgagcgg
    10381 gagcttacca gtgatgatga aaaccaataa accaaaccct gctaacatga aaaaactttt
    10441 actttttatt gcattaggac tttcagcttc tgttgcatca gcacaatgta cacccgatat
    10501 gtcggttgcc ggagatgccg gaatccatcc tgacagcgca acgaatttta tgcctgcata
    10561 tgttggtacg ccttacaatc aggttgtaac tgccgttgta cctgcagata cttgtgcgca
    10621 gatattgcca ttgccttttc catgtactac cctttcattt gattacattg aagtaaccag
    10681 cgtaacaggt ttgccaacag gattcacatt tacatgtgca cccgcaaact gtcagtttat
    10741 gggaaataca atagattgtg ccataatttc cggaacagca caaccaggac aagaaggtgt
    10801 ttatcctttg gtgtttaacc tcgatgctta cgtgggtggt cttggtgttc caaacagttt
    10861 taccttgacc tattaccgta tcgttgtaaa tccggcagtg ggtacaattg aactttcaca
    10921 ggatgccttt gcggtttctc aaaaccaacc aaatccgttt gatgaatcta cattgattga
    10981 acttgaaact tccgtttctg gtcttgttca atttgaagtg ttcaatctga tcggtcagcc
    11041 cgttcataca cgcaccgttt ctgtacagaa aggtaaaaac cagattcgtt tcgaagcagg
    11101 tgatcttcct gcaggaacgt atatgtacaa agtgtccgac ggaaaacgca gcaccacaaa
    11161 aagaatggtc gtaacgcgat aaataatttt aatctctttt agaaaccgtc cccttagtgg
    11221 gacggttttc attttatgaa gtttgaagtc acaatattag gttccggttc ctcaacacca
    11281 tcgttaagga gaaatcctgc ttcacaattg gtgaatgtac aggagaatta ttttctggtt
    11341 gattgcgggg aaggaacaca attgcagatg cgtcggttca agatcaagtt tcaacgcatc
    11401 gatcatattt ttatctcaca cctgcacggc gatcattatt tgggactgat cggattattg
    11461 caaagcatgc acttgttggg acgaaaaaaa gaggttcata tttatggtca ccccgatctc
    11521 aaaacgatga tcgatctcca tatgaaaatc tcatccaccc gacttactta cccattggag
    11581 tttcatgcat taaactatga tgctcccgaa ataatttatt cgaataaagt ggttgaagta
    11641 tctacggtga ttctaaggca tagaattcct acctgtggat ttatattcag ggaaaaaccc
    11701 aaattgcttc cattgagttc tgagaaaatc agaaagcata aaatccctgt atttgctttg
    11761 cccgcaatta aagagggcgc agattatact acggaaaatg gaacagtcat tcccaatcat
    11821 gaattaacat tgccccgacc accatcgcat tcatatgcct attgttcgga tactatattc
    11881 accgaaaaaa ttgtggagag tattcaggga gtggatgtcc tttttcatga aagcactttc
    11941 accaaagaat tggaagaccg ggcaaaggaa acttatcatt caaccgccgc tcaggcagct
    12001 actattgcac aaaaagcaaa agtcgacaga ttggtgctcg ggcatttttc tgtgcggtat
    12061 aacgatctgg aaccatttct ggaagaagcc cgagagcatt ttaaaaatgt tcaactggcc
    12121 actgatggaa ttaccatcaa tgtgtatccg cagtaattaa agcatccgcg catcctctga
    12181 aagttttgtt cctctcagaa attcttcaat acccattctt tttttacccg cctgttgaat
    12241 atcggtaagc agcagacttc catcatttgt cactacttca agaaaggtct ttccgtcggt
    12301 atggatggat ccgggtttgc cggttgaatt tcctttgcgg ctggcaaata tttttacgag
    12361 ggtttcttct tttccacttt gaacgagtgt ggtaaatgct gccgggtatg gcgacattcc
    12421 ccgaataaga ttgaaaatgc tgtccgtttt ttttgtccag tctattttac aatcctcccg
    12481 gaaaatttta gtagcaacag gaaaattctt cgttcccacc gacatctctt cctgtggaat
    12541 ggaatgtgcg gttccggatt caattgcttt aaccgtttca accagtaatt ctgcgccaac
    12601 taacatcagt ttgtcgtgca attctcctgc tgtttcattt tccccaatac tcactttacg
    12661 ttgcgcaatt acatttccgg tgtcaatttc ttcgcgtata aaaaaggtgc taactcctgt
    12721 ttctttttct ccattgatca ccgcccaatt gatgggtgct gctcctctgt aattcggtaa
    12781 aagcgatgca tgcaggttga ttgttccctt aataggcatc ttccagacta catccggcaa
    12841 cattctgaat gcgacaacaa caaaaagatc ggccttataa tttttcagtg tgtttagaaa
    12901 ttccggatcg cgtaattttt ccggttgtaa aacgggaatg ttgttttcgg tagcatacac
    12961 ttttacagct gaaggactaa gcttctgacc tcgtcccgcc ggtttatctg ttgcggttac
    13021 aactgcacag atttcattgc ctgcttttcg caaagcatcc agtccggcaa cagcaaattc
    13081 gggggtgccc atgtaaacta ttttcataca cgtcagattt taatgtcttt ccatttccct
    13141 tttcgtatat agaccaggca ggcaaaaaac atcagcagaa aataaacata ctcaagacac
    13201 cacacataaa cgataggctg tgggaatttt actgtcaaca accatgcacc aaacagataa
    13261 atggcaatgg agaacaattc gatgcggaaa gcagtttgtg ttgcaccggc accggcgatg
    13321 agtgcgttta gtggggaaga tgcagctaac agaaacatcg caccggttac aatttgtaat
    13381 gtatttgctg tggtgtccag aatttcagga ttcgttgaaa taaatggaac tgccagatga
    13441 ggaaaaaata aattcgggaa tgtgaagagt acggtcgcaa taagattcaa aaagataatg
    13501 cgttttacag tgggtaccac ttcatttgtt tttccttctg caatgagatg cgatacgaat
    13561 gttcgcgttg cagagcctaa tcccaatacc ggaatcaatg cgataaggta aaaaatgcga
    13621 atgacctgcg atacttcaag attggcagga cccaattgct cgatgaaaaa gaaaaaaatg
    13681 gtccatgtcc ccatcgaaac gattccttgc agcatcaagg gaaaagaaag tcgcatcagt
    13741 tccaatgaat ctgatgtatt gaagcgcata tttccaaacg gagaataagg tttcgattct
    13801 ttatcgatca gcatataaat gaccacaaac aaaagcgctg cagcttcagc tattactgtt
    13861 gcaattgcag ctccctgtaa tccgagttca ggaaaaccgt aattcccgaa aatgagtaaa
    13921 taatcaagca gaatattcag caaggcagta ataacagtag agtacatgat gatcctcgtt
    13981 ctggctactc ctgtgtagaa tccctgaaac gccatggccg gtaatgcaaa aaagaatccc
    14041 catgatcgga tatccagaaa ctcctgcatt tttattcctg tttcagcatc ttttacaata
    14101 gcaggtaaga atttatgtga acccacaagc agaagtatcg taaacaatac gcttaatccg
    14161 caaagaatca ataaagattg tctgaataca tttcctgctt cttttagttt taactctcca
    14221 tctctgcgcg caatcaatac ctgtgcgccg ttggaaatgc ccattgcaaa catggtgagc
    14281 gttacataca gcattcctgc atttccggat gcattaaaat caagcgaacc aatcctgttt
    14341 aaaaatgcgc tgtcggttat cagcatggtg aattgcacaa acacaccaac cataatgggt
    14401 gatgctaccg caaggatgcg accgtaactg agtggtgcgt ttaactggtt cattctatat
    14461 aaaggacaag tcctttcagg tattcgcctt cgggatgaaa aatactaacg ggatggtcgg
    14521 cgggttgatg caaacgatgc agtatgcgca catttctttt tgcattggct gaagctgaaa
    14581 ataccgctcc ggtaaataac tccatactga taacctgtga acaggaaaag gtaaatagca
    14641 atcctccggg tttaatctga cgaatggctt cttcattcag ccttctgtat ccttgtattg
    14701 cattgtgttt tgcacttaag tgttttgcaa atgcaggagg atccaaaaca ataatgtcat
    14761 aatcgttttc aatgttgcgg atatagggaa tcgcatctgc aacaatactc ttatgcttat
    14821 cagcgtgtgc ggtaagggca atatttttat cggttaactc aattgctttt tttgaagaat
    14881 caagcgaatg cacttcccgt gcaccttcat tgagtgcata caccgaaaac ccaccggagt
    14941 aacaaaaggt attcaggatt tttttccctt tcgcatacga tcccagtaat tttctgttct
    15001 cgcgctggtc gatgaaaaaa ccggtttttt gtccgctctc ccaatctacc acaaaacggt
    15061 gaccgtattc catcacttca gtacttcctg cagcatctcc atacaaagaa agcgatgtgc
    15121 cttccgtatg ggttttggga agtgtttctg tgctcttatc gtaaattcct ttgagcgaat
    15181 tgccaactac agctttcaac gcttcaacaa tctcactgcg ttgtttgtac atgccaatcg
    15241 aatgacattg aacaatgcaa tagccggcat aataatcgat caccagtccg ggtaagccat
    15301 ctccttcgcc gtgtatcagt cggaacacgt tggtgttttc cggacaaaca gcattacgca
    15361 tattccatgc tgctgcaatt ttagctttcc agaaaccgcc atcaggacgt gtttccccga
    15421 attgcaacat tcgtatgctg atgcttccgg tttggaaatg tccatacccc aacgttttac
    15481 ctgagcgatc gcagaccatt accatttcgc cttcttccgg ttgaccgtca atacggtgaa
    15541 tggcgccgga gaacacccat ggatgaaagc gttccagtga tttttccttg ccgggtttga
    15601 gctgtatgat ggaatttgtc atttggagtg caaagctaca taaatgcttg ataagctgtt
    15661 tttcgattta ggcatggatt tggtatgttt gcatccccta tgaaactgct tgctatcagg
    15721 gttctgcctt ttttggcttt atttttcttt tcatgcggat cggaatctcc cgttccggat
    15781 gaaattgttt cggatacact catttctcct gagtatgcag ataccaccgt atccgaaaat
    15841 gaaaatacag aaaccctttc cagtcggccg gatcccgaag aattattgga atcctttatt
    15901 tactgtaccg agagtgcaga gaattcaatg acctgtaaat acttcatcgc aaaagccatt
    15961 tgcatgtatt atggcataaa cgattttgaa tccgatgaag ggacctacat taattttgag
    16021 gagatcttac cccgaataaa atcctcagac aagtggtacc gtgcaggtga ttcctttaat
    16081 caggatgttt tggtaaaagc acaggatgca gttaataatg gaaaagcagc acttgcaatc
    16141 agcgaacagg agcaatacgg acacgtggtg ttgatcttgc ccggtaaaac ggaagcagct
    16201 ccggcctgga aaaatctatc ggtgccacag gtagccagtt tttttatggt gcccggtatt
    16261 caaccgtttg tgaatcgttc aatggcattc gcatggcggg ctcctgaggg aattggtatt
    16321 tatatccgaa aataataatc atgatacgca cagaacaaga agtacttgcc gatcttttaa
    16381 actacaaccg gaaatcaaca ttgggttatc tttccaaaat caaggaattt gatctgacca
    16441 aaaccttttc tgcggaaggg aaagaattga attctcctat atggattctc ggtcatctgg
    16501 ccaccactca aaactatttg tgcctgaaaa gtactggtgc cgaaatggtg cgcttttcct
    16561 gggcgaaatt atttggaatg ggttccgtaa ttcctgatgc cgctggtctg cctccggttg
    16621 aagaaattgt gaatgtgctg aatgaagttc atcaacgttc gctgaccaga attctatcat
    16681 tgagcaacga ggatctcgct cttcccacaa ctacaggttt tgcaatgggg ggcgaggata
    16741 gctacagagc aataattcgt cacgcaatca tgcatgaagg aattcatgcc ggacatatgg
    16801 gctggatctg taaactttgg ggtgtgaaaa ccatttagaa attgaaaaaa aaatccctca
    16861 ccatcggtga gggatttttt ttggaattat tttttaatgg attatctcaa tagtgagacg
    16921 tgaccgtaat aaactttttg ctgaccggtt aaggcatcct gcaatttgat tttccacaca
    16981 tacacatcct gtttggaatc gtttccacga tgttttccat cccaacctac agtaggattg
    17041 tgtccttcat agatcagttc gccccaacgg ttgaagatca tgaattcgta cctgtttgga
    17101 tcgggatctg caccgttaac gataggaagg aagatgtcgt tgattccatc tccatccgga
    17161 gtaaatgcat tcggaacgta aaccaatagc tcatcgtaaa cggttacaat atgacaggtg
    17221 ctgtcgatac aaccaaactg agatactgca gttaaacaaa ccacataggt attaggcgca
    17281 ttggaaggga atacaaatgc aggattttca tcagttgaag atccgattcc tgcaaaatcc
    17341 cacaggtaag tggttgcacc ggtcgattca tttacaaaat tcacgtttgg tgcaagtaca
    17401 gtaggttgca taggaatata cgagaattcc gcaaccggat taggataaac acaaactgca
    17461 tcaacaatgg tagtatcatt gatacatcct tccggtgatg tgatggttaa attgatatcg
    17521 taacatccag gatcggggaa ggtttgcgtg atgcttgcac agttggattg tgttcctaaa
    17581 acaccaagat cccaaaggca attgccacca atttgaccaa ccggagtaga gttaaccagt
    17641 gtggttgtaa atggtgcaca tcctgcagca acagttggtg caaaatttac ctgtggtaat
    17701 tgatgcattg taacgtttac ttgtagcata actgtatcca tacaaatatc cattgtcaac
    17761 aactggtagg tggtatttac acttggagaa accgttgtag tctgagaagt atctccacca
    17821 atccactggt aatagtaagg agctaatcct tgtgatccct gaccggaaag cgttgtagga
    17881 gttcccggac aaataacaac atcaggagaa atcgtagcag tgtacaatgg atacacatct
    17941 attgttgcgc tttcagtagg agatatacaa ccattggcat ctgttgcaaa aaccgttact
    18001 gtagtagttg tgctggatgt tacattaaca gatgaagatg tttcggttgt gcctgaccag
    18061 gtataggtaa acggaggagt accgccaata ccaagtgcaa tcaggttgaa cggttgatca
    18121 gaacatgcaa atccacctgt tggagggaat aagtcgagaa cagaaggttc aagaactgtt
    18181 actgtagtat cagcacggca acctgttgcg tcttgtgcta tgatgttata tactcctgca
    18241 caaacattgg taaagttgtt ggccggctga taactggctc caccgtttgt tgaatagcta
    18301 actgcaagtg ggctgtttac agttatcgtt gcatcgcaag cgccattaca ggttacattg
    18361 gtgattacag tagatgtaat aactacctgt ggaggttcgt tgatggtgaa gttctgactg
    18421 attaaacagt tattatcatc tgttacggta agcgaataac ttccgtcgca aatgttggtt
    18481 gcagtggcct gtgaaggacc ggcaagactg ttcgaccagt tgaaatgata caaacctgca
    18541 acagtaccac cggttgggaa tgcctgaact tgtccgtcgc aaacatcgaa gcatgttgca
    18601 tcaaccacag tgaagctttg gaaggctaat tgcgttggct cgtttacagt tgtagatccg
    18661 gttacagtac aaccattgtt atctacaatg gagaggttgt aatttccgcc ggcaataaag
    18721 gttaatgatg gtgaggctcc aaatgtaact ccaccgtcaa aactatattg gtagggtgat
    18781 gttcctccgg ttgccgacat caatattcca ccggtaaagt cattgaaaca aagaagtgga
    18841 gtaggtgtgg cagtaagtgt cagtacagca ggctcaatca tcgtaaagac gaaagtgctg
    18901 gtacatccat ttaaatcaac tactgatgca gtatgattac ctgcgccaag accactgaaa
    18961 ttcggggatg cttgaccagg tccaccgtcc attgagaaag tgagtggagc tgttccgcca
    19021 tttaccaaaa cctgcgcaag tccgtcagtt gatccaaaac ataatgggtt agttccgata
    19081 accccggaaa ttaccggaga ggaagcgtca ataagagtga tggtttcagt tgcagtacat
    19141 ccgtttacat cggtaacaac aacaaagtag gtgttttctg caagaccgtt aaacgcgttt
    19201 cctgcctggt tggttgttcc gttgtcgatg ctgaactgga atgcacctgt tcctccgttt
    19261 gcattgatgg tgatgcttcc gtttgcttgt ccgcatgtag tgtttaccgg agtggaagta
    19321 aatgtgacct gagtaggttg gcctatgatc tgtgttgtat taaacaaaca accgttttga
    19381 tctttaacct gtacattata cgtgcctgct gttaaaccat taatcgtgtt ggaaatctgg
    19441 aaagttgttc cgccattttc tgaatactgg taaactggag ttcctcctga tgcgttaatc
    19501 gtcatcgatc catcagcacc attaaaacag tcggggttat caggtgttac tgttgctgtt
    19561 aatgcagcag gttctgttat ggttgctgtt ccggttatta cacagttatt cgcatctgtc
    19621 acacaaagcg tgtaagtgtt ggcagctagt ccaaagaagg atgagaatgc ctgcgatgtt
    19681 gtgcagttgt tattgctaaa ttgataaggt ggtgttccgc ctaccgcacc aatggaaagt
    19741 gttcctgagt tgtttccgtt acaggtaaga tccgtgtgac tttctgaaac aactatttgt
    19801 gttggctggt ttacagtggc agttgctgat tctgtacatc cgttttggtc acgtaccatc
    19861 agattgtaag tgccggcagc taatccggtg aaatttccag ttgcctggta ggttgctccg
    19921 ccatcaacac tgaattggaa aggaggagtt cctccaattg gagggtcaat tgtaattgtt
    19981 ccgtttgatc ctccaaaaca agatacgttg gttgaactgt ttgtaaatat aatttgtggt
    20041 ggaccaatta cgttgaatga tacaacataa acacaaagcc cctggtcggt cattgttaca
    20101 gcatgcgagc caggacaaag attgaaaaag tccccgctac caaatgcgct ttgtgaaccg
    20161 ttatccaaat caaatgaaat tgttccaaaa ccacctgtcg cactggtgta aacttcaccg
    20221 tcacaaagtc cgggacaact tacatcaaca attgaattca atacacccga catttctacg
    20281 tcgttcacgc cgacaaatgc aatttgctga caattgtttg cgtctgttac caatacttca
    20341 taagcaccgg caacaagttg gtcaaataaa cctgtaccat ttgctatacc aatagttagg
    20401 ttgttatagc tataaggtgc aagtccgcct gaagcctgaa cctggaaact accattgtta
    20461 aaaccacaat tcgattcaat tgaagagatt gtattgactg catatcccgg aggatctatc
    20521 aatacaactt gtgacgttac ctggcaaccg tttgcatcac gaatgatcat gttttgaact
    20581 ccgctgcata aaccagtgaa ggttgttccc gattgaaatg caccaccatt aactgaatat
    20641 tggtaaggag cagttcctcc tgaacttgca cttgcagtta cctgaccatt acaaacaccc
    20701 tgacaaagcg ggttcgttcc tgtaaatgtt gcagcaactt gtgtaggttg tgttattaca
    20761 acgttggcgt ttacctgaca cccatttgca tcacgaacaa ctacgttaat tgttcctgcg
    20821 caaagattgg taagcacatt ggaagcctgg tatgtcgctc cgccatttga ggagtattcg
    20881 taaggaggag ttgcgttgct tgcattaaca gtaatttgac cattacactg accaaaacag
    20941 gttacatgta caggtacaga agagaatgca agctgtgtag gttgagttat tgtaaatgtt
    21001 gttgtagcag cacagttatt ggcgtcagtt actataacag tgtagtttcc ggcggcaagt
    21061 gctgtgaatg aattagaagc ttgcggtgga ggatttggtc caacagcagg gttgaggtcg
    21121 taactaaatg gagcagttcc tccggtgatt ccaatgagcg cagaaccatt aacacctcct
    21181 gcacaactca cgttggtttg gttggttaca cttgcgattg gggtaagtag cgcattgatg
    21241 gtaatattta ccggtgttga tactgtacaa ttgttggcat cgcgaacaac aatggtataa
    21301 ctacctggca ataggtttgt aaatactccg cttccaacaa atggaccacc attcaatgaa
    21361 tagttatagg gggcagttcc tcctgaaccc acaatggtaa cactaccgtt ggcttgtccg
    21421 caggtagagg agactgttgc tgtaatcgtt ccaacaacct gtgtaggctg tgtgatcgtt
    21481 gtgtttaatg tgatctgaca gttatttgca tcccgaatga tgatggtgta gttacctgga
    21541 gcaagtccgg taaaactatt tcctgcctga aaggctccac cgttaatact gtattgaagc
    21601 gttccagttc cacctgaggc gttaactgaa atctgaccat tgttcaatcc aaagcaggtt
    21661 acgttggttg gtgttagtgt tcctgtaaga gctgccggtt ggtttacggt aacagaagat
    21721 gtaacagtac aaccaccttg tgcggttaca gtaacagtat aggttccggc tgtaaggtta
    21781 gaagcggttt gaccggtttg tccaccggga gaccagctat aggtgtaagg tggtgttccg
    21841 ccagctggag taacagttgc tgatccgttg ttgccgccgt tgcaaagtac atgcgttgct
    21901 gctgttgttg cagttaatgg agcaggtcct gtaaccgttg ctgatgcagt agcagtacag
    21961 ccattggcac ctgtaactgt tactgcatag ttacccggac aaacattcgt tggatttcct
    22021 gttgccaaac cgccggtcca ggcaaatgta aagggtgcga ctcctcctga tgtattcaga
    22081 tttatggagc cattacatcc acaagtagca ttggtgctga caggattcaa tattggcgct
    22141 gcacctgtag aagtaacagt gattgtttga gtaattggag gtgtacagga aagcgggtcg
    22201 gtaactgtta ctgtgtaatt accgggagca agattcgaaa tagtattggt tgtttgtcca
    22261 ccggggctcc acaaaatgga gaaaggtcct gttccacctg aaagcgtaac acctgcagaa
    22321 ccattggatg ctccacatgt agcaggagtc atgctgggtg ttagtgttaa tggcggagta
    22381 gtacaggaaa aactctgata gcctaaaaac aagcctgagt cgtaaatttg gtcacctgca
    22441 tcagcgataa taattttgat gtggtacgac tgacaaggtg taacgttaat ggttgcaaca
    22501 agtccgtcag tataaccatc atactgcatg attcctgtgg tattggtatt gtaaaacgct
    22561 gcgttggttg ttgcgtttac attgtcgata ctcaccaact gattattggg aagtcgtgca
    22621 aaattgaagt tggtatagtt acctcccaat gggttgggcc ctgtaatgaa catcccaaaa
    22681 ccatcgttga aagaagaact cacgaattca ggatattctt ctgaaccaaa tacaaatgca
    22741 acattgaatt gagaacaggt tggaatcatg tcaaattcca gaatacagtt atcgaaagaa
    22801 ggcgcaggag aaccggcacc gggttgggtg gtaagctgag ggtcggaaaa gtcaaaacct
    22861 gcaacagttg ctccggcaga accgctgttg ttaggcccat ctgctgctgc tgccgaacca
    22921 gtggtaagga tgattcctcc attggtcata cccacaccac ctgcaccgag gttaccggac
    22981 caggttccgt aggctgttcc gccacaattg atcaccacgt tcgaaacagt gattccctgt
    23041 ccaacaaggc tattgatgat ggttgcagaa gaaccaccag gggtaatggt aatctgagct
    23101 ttggaagaag aagaaagaaa cacgaaaaga cagattagtg tgatcgtctt taaagtgttt
    23161 gaaatcaggc tgtttggctt gatcatatcg cttggtttgg ttttgggcaa cgttgtaagg
    23221 tagtttacct gatgtcaaga tgaaaaaata ccattttggt tgccttaaat atcggtcaaa
    23281 tattggttag tcagcaagtt tatatttaaa tcgtttttag tgttttttgg ttataaacgt
    23341 ttgaattata attgcttgtt ggtttttttg cctccttaac acggaagtct ttttttgtaa
    23401 aaaattaaaa ttcaattttg tacaaatgaa aagtggaaga aaatcagata aaatactaat
    23461 taatgatttg attttcagat gttactctgg gaatgcttcg gtcgtatgg
//
LOCUS       DAOMYG010000005        19617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_964_length_19617_cov_11.741233, whole genome shotgun sequence.
ACCESSION   DAOMYG010000005 DAOMYG010000000
VERSION     DAOMYG010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 19617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19617
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_964_length_19617_cov_11.741233"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1241)
                     /locus_tag="PK610_00405"
     CDS             complement(99..1241)
                     /locus_tag="PK610_00405"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRJ34412.1"
                     /translation="MPFSKLGLSDALLKSLAEQNYTEPYPIQQQAIPAILKGKDILGI
                     AQTGSGKTAGFVLPILQQLQDGVSTKNRHVRALIVVPTRELAIQVNEVFTTLGKHLPR
                     PIKTMAVYGGVSINPQMIGLQGVEILVATPGRLLDLISTNAVSLSAIKILVLDEADKM
                     LNLGFKEEMTKIFELLPKQRQNLLFSATLSDDLNNINRNLLRDPLVIKVEEEIQNLDL
                     IKQVAFTIAEEKKGPLLRYLIREQNIEQVLIFTSSVHRTDAVTLKLNQNGINAIAIHS
                     KKSQDARNQALELFKSGKVKALVATDLLGRGIDIKYLPYVINYELPRSPKDYIHRIGR
                     TGRAESSGEAISLISPEEEHHFKVIQKKMGKRVALTDGNEINLKGY"
     gene            1350..2663
                     /locus_tag="PK610_00410"
     CDS             1350..2663
                     /locus_tag="PK610_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009086024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRJ34413.1"
                     /translation="MNFSSILSHFNIKELNEMQKATSVAILKQNDVVLISPTGSGKTL
                     GFLLPILQLIDKDKVGVQVLIIVPSRELALQIEKVFKQMSTPFKINCCYGGHSVRVEE
                     NNFQHPPAVLVGTPGRISHHLSHKNFLVDTIHTLILDEFDKSLELGFKEEMEFIIQQC
                     KRLKKRILTSATALENVPAFVGLKHSVELNFTTDHKDLQSALVVKTVRAESDDKLHAL
                     MLLLGKIQAKSVIVFCNHREAVNRISEQLRSYKVDHGFYHGGLEQIDREKTLIKLRNG
                     SISLLITTDLAARGLDIPEIEAVIHYQLPTTEDTMVHRNGRTARMSAQGTAYFLLGVN
                     DHLPKFLKVTPKEEPLPDKLELPVPCEWRTLYIGAGKKDKINKMDIVGMLLQKGQLKK
                     EELGKIEVLDYSAYAAVKANKIVKTLQLIKEEKIKNRKVKMEIST"
     gene            complement(2786..3622)
                     /locus_tag="PK610_00415"
     CDS             complement(2786..3622)
                     /locus_tag="PK610_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HRJ34414.1"
                     /translation="MIQAGTIQQMRVLRIETDKLILKSEQGQEAVLLKRELKEIPQLN
                     DELEVFIYSDSNHERLATVKRPNLLLHEFGLLQVKEVNDNGAFLDWGIGKDLFVPFAE
                     QKNKMQEGKWYLVFLYADEQNGRLLASAKLNKFLSNDQPELQARQEVDLTVMEKTDLG
                     IKMIINNKYTGLVFYSDLIRPLRTGEKLKGYVHQLREDGKIDLRLRKPGAQEIRSSES
                     ILLEAIKENDGFLPLHDKSDPDDIRDTLNMSKKTFKQAVGALYKRKIITLSDQGIHLV
                     KE"
     gene            3721..4599
                     /locus_tag="PK610_00420"
     CDS             3721..4599
                     /locus_tag="PK610_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRJ34415.1"
                     /translation="MRKFPYTEILGWSASRYDVFMSCKRNYFYTYYSKHDKEFSKERI
                     NRLRSLTTIPMEAGSLAHELIAAVLHRLQKSSMPINRQRLFVFLSGIVEETVSKKEFF
                     EVYYKRIDRVDMDQLVDIALKSILQFLDSDRFAWIVERAQNRSDAWIIEPEGFGETRI
                     AGLKAYCKVDFMLPDRGKVYIFDWKSGKPEPEKHRKQVIGYALFANHNYGYDPKTIFP
                     VLAYLKDNYHEEQIAIAENELSQFQQQVKTESEEMWAFTKDVNRNIPLNKEHFPLLSN
                     PALCAYCNFKELCGRA"
     gene            4922..11371
                     /locus_tag="PK610_00425"
     CDS             4922..11371
                     /locus_tag="PK610_00425"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HRJ34416.1"
                     /translation="MNRFYQSLAALMLLLATGGMSPLWGQTTLINPSTDGGFELGSTL
                     ADNGWVAVNGAQTNKWFVGGVATSFAGANSAYISSNTTGTTYDYDVNSASGVSFYKDI
                     TIPAGESKLQISFRWKSAGETCCDYLRVWLAPVSFTPTAGTQTATGTNISGNLNAQTV
                     YQQFNSTLPVIPGQSYRIIFEWWNDGSLGPSPAASVDDISVVSNVPGTFISVATGNWS
                     AAATWDANAVPTAADNAIVSAGHTVTVNQSGTIINALTVDGILAYGATPTNFTITNNL
                     SVSSLGVVNVFNGTTGKTLLVGGNIVNNGSIDISVGGTTGGNLTLNGAQVQTVSGTGT
                     FVNSIIRNLTCSNTNTATPNINWLVNDIKIAYNLNISGARINLGTNKLTWGNNAAGNT
                     FTAPAGTGFLAGGKFSRWWTTGQTGSAITAGAEPTTVTSRYPFINAAGQSRAIYISRS
                     SSTVTGNVAGELAVVYSDAATVTTSLNIDDAGYIVTDRFDGSWLVTAENGYVYVSGTH
                     TVVPIATGAYPAANGNSRVLTSNAVIGGTHQNGTSGPVAQRIGLSTAELTIPTGIYIG
                     ANTTDIFQPCSGAPTSSNSISSVATVCSGVNFNLSLSIPYTETGITYQWQSSPDGVTY
                     SNISGATNATYVATQTSATFYQCIVTCVNSGQSITTTPVQVLQNNFQTCYCTPTYSNG
                     CGGTGADAITNVTLGGLNNSSGCAATPYYTYYNAVTVPDLVRGATMPLSVSFGSDGSQ
                     FAGVWIDFNQNGLFSDPGEFVAFNTVTAGASGTVNFNIAIPAGATLGVTRMRIRGGND
                     TQLLNTPCGASSSTWGETEDYNVNLIDAIICAGSPVAATTNGPVSVCSGVNFNLTLSE
                     DYTSLFGITYQWQSSPDGITFSNISGATNATYSTSQTATTYYQCIITCGPSGQSTISV
                     PVLVNTYSACLSMPVSGSLATCDAKFYDSGGPAGNYGNNENRTFTFFPGTPGGVVVAT
                     FVSFATEAGWDYLRIYNGNSTAAPALHTGSGFNGASINPTVFTSTAADGSLTFQFTSD
                     GSGTPAGWDATISCILPPTDPSAPVQDPAMPSCIAGTDLSVPGTPPAGDAWYWQTAAN
                     GTSTSSPVSGIFTVFQNGTYYVRSFNSTTGLWSVNSTSITVSNIPVAAAPPAPVAAAS
                     PACLTTTISVAAPVDPNVTYFWQGTVANGIDNSNDAASPFTVTASGTYYVSAFDVSTG
                     CWSEGVGVAVLVDTYVPDAPTAASPFDFCIGAASASISATAPSSSSQIATSGTISIPI
                     PDNDLTGISNVLAISGVPSGAVVASVAVTVNIPHTFNADLDIYLTGPNGTTIELSTDN
                     GGSSDNYTNTVFSNLAINPITGGTGPFTGLFLPEGDLTTLFSLPNGNWTLRVADDLGG
                     DLGTLTSWSVEVAYTVPVSTVTWYDAASGPSQIGTGNALESIGTTTLPNGNTPGTYSF
                     YAQSESGACSSLTRAQVDVVINPFSATVTAVDVTCNGANNGTFTVTAPNCGTAPYEYA
                     VDGGAYTSTIPANLTPGAHTVMVRDDNGFETALYSITITQPAQLIASETHVDVLCNGA
                     ATGSIDVTITGGTAPYAFTWSNGAIGEDQTGIVAGTYNLVVTDNRGCNAFASVNITQP
                     TAVVIAETHNNLNCNGDASGSIDLSVSGGIAPYTYSWSNGATTEDLSTLAAGAYVVTV
                     TDDNACTSTLSVSITEPAVLAAVVATATQPSACGATDGALDVAVTGGVAPYTFAWSNA
                     ETTEDISAIGAGLYTLTAADDNGCFVVLNTSLSDPNAPVAVVDLVNNILCFGAATGSI
                     DVTVSGGVSPYTYFWSGGSTSEDLSNATAGVYTLTVTADDGCQTFASATITQNAEIVI
                     ASVTTSTLCNGDNNGAIDITVTGGVVATAYDYSWSNSSITEDITALAAGNYTVTITDD
                     NLCTATSTITVTEPAVLAAVGVVTNVSCNGLGNGSINLTITGGTPAYDVLWSSGAVTE
                     DVSGLAPASYTVTVTDDNACVATASFTITEPTVLGATATSTDEINGNDGSVNLTVTGG
                     STPYSFAWNNSATTEDLSGVVAGNYSVTVTDANGCTATVSITVGSQVGVNELGVRVIN
                     IYPNPSNGIFYIEMNGQPESGDRIFVRDALGRLVLEENIMQNRITLNLDTQERGFYLV
                     EVWSAGQRSVHRIQLQR"
     gene            11502..13325
                     /gene="recQ"
                     /locus_tag="PK610_00430"
     CDS             11502..13325
                     /gene="recQ"
                     /locus_tag="PK610_00430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HRJ34417.1"
                     /translation="MHSTLHSPEEILRSWFGYDSFRGQQKKIIDTIVAGKDAFVLMPT
                     GGGKSLCYQVPALVMPGTCLVISPLIALMKDQVQALRINGIEAAFLNSSLGAEEEYSI
                     LQKCGEGKLKLLYTSPERALLMPQSFWKKVNVNLIAVDEAHCVSQWGHDFRKEYTRIG
                     ELRANFPDVSMVALTATADKLTRKDILTHLGLHQPEVFISSFDRPNLSISVRSGLRSA
                     EKNDLLFSFLAARRNDAGIIYCISRKMCEVLSGMLFERGIRNAFYHAGMESVERARVQ
                     DAFLHDEISVVVATIAFGMGIDKSNVRWVVHYNLPRNMESYYQEIGRAGRDGLPGDTL
                     LLYGARDLGMLGYFATQSAQPELQLEKLHRMQYFAESRICRKRTLLSYFGEKLTRDCG
                     NCDVCLHPPRFSDHTKTAQLVFTLIERCREQLSLGILVGVLRGSNAEEIRSRGFHLLQ
                     QFGAGRERPEHEWRQVIYQLTGIGYLEVAYDDHHALRLNPSAQEVLAGKGRVQLAEIP
                     SRKTERNRPVPEILVAHQRDADSGELFEALRKLRLEIAREEKVAPYIVFHDKSLREMC
                     DRLPLSESELRSISGLSDRKMEKYGERFLSLLREWESFSQS"
     gene            13369..14286
                     /locus_tag="PK610_00435"
     CDS             13369..14286
                     /locus_tag="PK610_00435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRJ34418.1"
                     /translation="MTNRRSNRLILLLFLMFFYILAQLGWWAYLISRLTRVVHADHPR
                     LEQRILMVWGEGLVFALLLLAGFIFTYRSYLKELRLARIQRNFLLSVTHEFKTPIASI
                     RLQLETLLLRKVDEEKQKQLLERAIRDIDRLNGLTGNILTASRIEQNEFPVFRSTADL
                     SAFLVASIEHLQTTVGKDHTTQVNVSPAVIFSFDADAMNSIVGNLYENAVKYSPNGST
                     IRIALEKRNERIRLTFEDEGNGIPEKEKLKVFARFFRSGDENTRSAKGTGLGLYITRH
                     FVLSHNGKIFIENCQPNGSRFVVEFDSKA"
     gene            complement(14338..16497)
                     /locus_tag="PK610_00440"
     CDS             complement(14338..16497)
                     /locus_tag="PK610_00440"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HRJ34419.1"
                     /translation="MKKLLLATITLLSCSNAFSQLVVNNTTLTPAQLVQNVLVGTGVT
                     VSNVTFNGAPGNTVTVQAGQFNGSSSNIGLAGGIILASGNAQVAVGPNNNGGASQGPP
                     QGIMATDPDLAAITPNQIYDQAVLEFDFVPIGDSISFRYVFASEEYNEYVCASVNDAF
                     GFFISGPGITGPYTNNAKNIATIPGTNTPVSINTVNLGVAGSAGSPGNCNSIDPAWAS
                     YNVFYAGTNTQNSVQYDGWTVVLTAKTAVQCGQTYHIKLAIADAGDGVWDSGVFLEGG
                     SLTSIGVDVTVATITGNNTIIEGCSDATFVFSRPSDSTDLTIAIAISGNATNGVDFSF
                     LPDSVYFPVGVDTVNLIVNPIADGVIETTDTLIITVYTITPCGDTVIKTAELYIVDPV
                     PINAEAGPNQNPTCPGQAVNLAASATGGNPPFNYTWSSGAATQNTTVNPMVTTMYYLT
                     VTDICNLVGTDSVLITVPIPTPWNISSQDETTLCPGDPANLSVTVNGGGYPPYTYNWS
                     SGGTTTSTTVSPVTTTTYNFTVTDNCNLDTIIPVIVNVPVYQPLVITISDEQLCLGEG
                     LQVTPVVSGGAGGNLFGWTGPTGTSFVIDQGAGTTFFTNPQNGDYIIMVTDQCSTIAM
                     DTAAYTFVGCEIIIPNVFSPNNDGSNDLWYITNLEYHPNTAVKVYNRWGLVVFESDNY
                     QNTWNGGDVPDGTYFYIVVPQREGYGPYNGTVTILRH"
     gene            complement(16635..>19617)
                     /locus_tag="PK610_00445"
     CDS             complement(16635..>19617)
                     /locus_tag="PK610_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ34420.1"
                     /translation="GEVVEYIPEAYQMIDGQKKLISCNYQLKNGIVSFQLGRNYNPNL
                     ELIIDPTLIFSTFTGSTTDNWGFTATYDNSGNLYAGSVAFGTGYPTTAGAYDNSFNGG
                     TTDIGITKFSATGNTRLYSTFVGGSNSEAPHSLMVNAAGELHIFGTTSSSNFPVTAGS
                     YDNSFSGGTNITINGISYSAGVDMIVVKMNAAGNALLASTFVGGTANDGVNTAATLNF
                     NYADQMRGEIILDAAGNVYVTSSTLSNNFPTTAGSLSQTLSGTQDGCAFKLNAGLATL
                     VWSTYIGGSSTDAGYAIKVASNGSVYVGGGTRSTNLAVSAGTLHATAPGGTADGYIFN
                     LDGNTGASIRGTYIGTNNYDQVYLLELDDDDDVYVTGQTKGTYPITPGVYSNGTSKQF
                     IHKLTPNLNTTVYSTVIGNTTGTTINISPTAFLVDDCENVYLSGWGGNTNNEGTTTGL
                     PVTSDAYDATTDGSDFYFFVLERNGGSQLYGTFFGGTSAEHVDGGTSRFDPNGTIYQA
                     VCAACGGGNTFPTTPGVVAPNNGSTNCNLGAIKMEFSYTGIVADANAAPNIVACGAPY
                     NVNFQGSSSGVHHIWNFGDGSPNALIQNPIHTFNAVGQYDVMYIAIDSSTCNIADTVF
                     LSVELLPSAIFAAEFDIPPFDPCQPGGLTVDLQFTGSGADSLSWNMGDGTIIADNLAV
                     SHTYSTPGTFIITLTAWDFLCNRSETFTDTVVYNANTVFANANVSPNIIACDPPYNVS
                     FVSTSTAPQHFWDFDDAANSTSTQQNPNFTFVDVGNYNVMYIAIDPTSCNLADTAYLS
                     VTILEPKVFNATLSSTPVPPCTDSVFMNIQFTGTGADSIIWNMGEGTIFVDDTLIDFT
                     YTTPGNYTITMTAFDLLCNRSEVITLNVDVQGSAAQGVPVIPNVFSPNSDGKNDQFVM
                     FYSNLPGVDALASLDVYHMEVYNRWGVKVFESDDSIKRWDGKIKGDPASEGVYFYICT
                     YQRKCWDPEPTTVNGHVTVTK"
BASE COUNT         5347 a         3948 c         4395 g         5927 t
ORIGIN      
        1 cctttatgtt ttagggagta ctttattcag tcttcacatt gtagacataa caggtttacc
       61 gattcatata attggtaaaa acataaacaa ctgatggatc aatatccttt taagttaatt
      121 tcatttccat cagtcaatgc cactctttta cccatctttt tttgaatcac tttaaaatgg
      181 tgttcttctt cagggctaat taaagaaatg gcttctccgg aagattctgc tcttcctgtt
      241 cttcctattc gatgtatata atcttttgga gatcgtggta attcataatt aattacatag
      301 ggtaaatact taatatcaat accacgacct aataaatcag ttgctactaa tgcttttact
      361 tttcctgatt taaaaagttc taaagcctga tttctggcat cctgactttt tttactgtga
      421 attgcgatag cgttaattcc gttttgattt aattttaatg taacagcatc tgtgcgatga
      481 accgaggatg tgaaaattaa tacttgttca atgttttgtt cccggatgag ataacgtagc
      541 aatggtcctt ttttttcttc tgctattgta aaagcaacct gtttaattaa atctaaattc
      601 tgtatttctt cttcaacttt aattacaaga ggatcacgta ataaatttcg attgatgtta
      661 tttaaatcat cacttagcgt tgcggagaac aataagtttt gacgttgttt tggtaacaac
      721 tcaaatattt ttgtcatctc ctccttgaaa cctagattca acattttatc agcctcatcc
      781 aacactaaaa ttttaatagc ggataaggat accgcattgg tggaaattaa gtctaataat
      841 cgtcctggag ttgctactaa tatttctacc ccttgcaatc caatcatttg tggattaatg
      901 gatacaccgc cataaaccgc catggtttta ataggtcttg gtaaatgttt tcctaaagtg
      961 gtgaacactt catttacttg tattgctaat tcacgcgtag gaacaactat caatgctctt
     1021 acatgtcggt ttttggtaga tacgccatct tgtaattgtt gcagaattgg caaaacaaaa
     1081 ccagctgttt taccggagcc tgtttgagca attcctaaaa tatcttttcc ttttaatatt
     1141 gccggaatag cttgttgttg gatagggtag ggctctgtgt aattttgttc ggctaatgat
     1201 tttaataagg catcggataa gccaagtttt gaaaaaggca tatgaaaagg ttttgagttc
     1261 aaatatacgg attgctcagt catgaatatt gagaaaatcc attcctgtag aatagcgtat
     1321 ctttgcatcc aattgaaata aatcatccca tgaacttctc atcaatcctt tcacatttca
     1381 atatcaaaga gctaaatgaa atgcagaaag ccacctcagt agccatctta aagcaaaatg
     1441 atgtggtgtt gatttctcca acaggttcag gtaagactct tgggtttttg ctccctattc
     1501 ttcaattaat cgataaggac aaggtgggtg ttcaagtgtt aattattgtt ccttcgcgtg
     1561 aattagcgct gcaaattgag aaagtgttta aacaaatgag cacaccgttt aaaattaatt
     1621 gttgctatgg tggtcactct gttcgtgttg aagaaaataa cttccagcat ccacccgcag
     1681 tattggtagg aacaccggga agaatatcac atcacctgtc acataaaaat tttttggtgg
     1741 atactattca cactttgata ctggatgaat ttgataaatc attagagctt ggatttaaag
     1801 aggaaatgga atttatcatt cagcagtgca agcggttaaa aaagcggatt ttaacatctg
     1861 ccacagcatt agaaaatgtt cctgcttttg ttggtttaaa acatagcgtt gagttaaact
     1921 ttacgactga ccacaaagac ttacaatcag ctttagttgt taaaactgtt cgggcagaaa
     1981 gtgatgataa gttacatgca ttgatgttgt tgttgggaaa gattcaagct aagtcagtca
     2041 ttgtattttg taatcaccgc gaagcagtaa atcgtatttc tgaacaatta agatcgtaca
     2101 aagtcgatca tggtttttat catggtgggt tagagcaaat cgacagggaa aaaacactaa
     2161 ttaaattacg gaacggaagt attagtttgt taatcacaac tgatctggcg gctcgtggtt
     2221 tagatattcc ggaaattgaa gcggtaattc attatcaact gccgaccacc gaagacacaa
     2281 tggtacatcg taatggccgg actgcacgaa tgagcgcgca aggcactgcg tattttttat
     2341 tgggtgtaaa tgatcatttg cctaagtttt taaaggttac ccctaaagaa gagcctttgc
     2401 ccgataaatt agaattaccg gttccttgcg aatggagaac actctacatt ggcgcaggaa
     2461 aaaaggataa gattaataaa atggatattg ttggaatgct cttacaaaaa gggcaattga
     2521 aaaaggagga gttaggtaaa attgaagttc ttgattattc agcttatgct gctgtgaagg
     2581 caaataaaat tgtcaaaaca ttacaattga ttaaggaaga gaaaataaag aacaggaaag
     2641 ttaagatgga aatttctacc taaaaattaa aagaggatgt attcaatatg catttactcc
     2701 agtcggcctt tcctgaaaaa attcacaatg atgaactact gcaggtgatt tgatttacct
     2761 gagaataaaa caaatttccg aatacctatt cctttaccag gtgaataccc tgatcgctga
     2821 gtgtgatgat ttttctttta taaagcgcac ctacagcttg cttgaaagtt tttttactca
     2881 tgttgagcgt gtccctgata tcgtccgggt cacttttgtc gtggagtggg aggaagccgt
     2941 cgttttcttt gatggcttcg aggagaatgg attcgctgct tctgatttcc tgtgctccgg
     3001 gtttgcgcag tcgcaggtcg atctttccgt cttctcttaa ttggtgaacg tatcccttta
     3061 gtttttctcc ggttctgagc ggacgaataa ggtcgctgta aaaaacaagt ccggtgtatt
     3121 tgttattgat gatcattttt attcccagat ctgtcttttc catcactgtg agatctactt
     3181 cctggcgggc ttgaagctcg ggttgatcat tgcttaagaa cttattcagt ttggcagaag
     3241 caagtaagcg tccgttttgt tcatcagcat aaagaaaaac caaataccat ttgccctcct
     3301 gcattttgtt tttttgctcg gcaaaaggaa caaaaagatc cttgccaata ccccaatcca
     3361 ggaaggcgcc gttgtcgttg acttccttca cttgcaaaag accaaattca tgaagcaaaa
     3421 gattcggacg tttaaccgta gcaagtcgtt cgtggttgga atctgaataa ataaaaactt
     3481 caagttcgtc gttaagctgc ggaatttctt tcaactcgcg ttttaacaga acagcttcct
     3541 gtccctgctc gcttttaaga attagtttat ctgtttctat gcgtaaaaca cgcatctgtt
     3601 gaatggtacc tgcctgaatc atttggcaaa ggtaaggata agaatgcagg ttaagcttta
     3661 agtaaggatt cgattttttt cattctgttg cggaaatgac attttgttta tcttgatcgc
     3721 atgagaaaat ttccctacac agaaattttg ggctggtcgg catcgcggta cgatgtgttt
     3781 atgtcttgta agcgaaatta tttttacacc tattacagca aacacgataa agaattttca
     3841 aaggaacgaa tcaatcgctt acgcagtctc accactattc ctatggaagc aggttcactt
     3901 gcgcatgaat tgattgcggc tgtacttcat cgtcttcaga aatcttcaat gccaattaat
     3961 aggcaacgct tattcgtttt tttatccggg atagttgaag agacagtttc aaaaaaggaa
     4021 ttcttcgaag tgtattacaa gcggatcgat cgggtggata tggatcaatt ggtggatatc
     4081 gcactcaaat ccattcttca gtttttggac tccgatcgct tcgcctggat tgttgaacgt
     4141 gcgcaaaaca gaagcgatgc ctggatcatt gaaccggaag gatttgggga gacacgtatt
     4201 gcgggtttaa aggcctattg taaggtcgat ttcatgttgc cggatcgcgg aaaggtgtac
     4261 atttttgatt ggaagagcgg aaaacccgag ccggaaaaac accgaaagca agtgattggt
     4321 tatgctttgt ttgccaatca taattatgga tacgatccca aaaccatttt tccggtgctt
     4381 gcctatttaa aggacaatta ccatgaggaa caaattgcca ttgcagaaaa tgagttgagt
     4441 cagttccagc aacaagtgaa gactgaatcc gaagaaatgt gggcatttac caaagacgta
     4501 aatcggaata tacctttgaa taaggagcat ttcccactgt tgtctaaccc ggctttgtgt
     4561 gcgtattgca atttcaagga attatgcggc agagcctgaa gcgggcaaaa ttgagcgggc
     4621 caatctacaa tcgtataaac gaaccgctat attgatattt taacaaaatg ttatttttca
     4681 tcacgacttc acggtttctt tttataaaca acaaaaattt aaagcaaaag cacatttatt
     4741 attaaaattc agcctgattg ttaagcgatt gttgacatca ttaaagtgct ttttttggaa
     4801 ttatgggttt gatggggcta cttttgacgc tcctattttt ctcaagcctg aaccaaaatc
     4861 agattgaggt gttgaggagg aggagcttaa tgtttaagtt gaattagcaa aaaccaaaca
     4921 aatgaataga ttttaccaaa gtttagcggc tttaatgctg ctgctggcaa ccggagggat
     4981 gagtcctctg tgggggcaga ccaccctgat caatccttca actgatggag gttttgagtt
     5041 gggttcaacc ttggccgata atggttgggt tgcagttaat ggtgcacaaa ccaataaatg
     5101 gtttgtcggt ggtgttgcta cttcttttgc aggagcaaac agcgcctata tttcatcaaa
     5161 cacaaccgga acgacttatg attatgacgt gaattcagcg agtggggttt cgttttataa
     5221 agatataact ataccagctg gtgaaagtaa gcttcagatt agtttccgtt ggaaatcagc
     5281 tggggagact tgttgtgatt atctcagggt ttggcttgct ccagtttcgt ttacacctac
     5341 ggcaggtact cagaccgcaa ccggaacaaa cattagcggg aatctgaatg ctcaaacggt
     5401 ctatcaacaa tttaattcaa ctctacctgt aattccgggg caatcttatc gaattatttt
     5461 tgaatggtgg aatgatggat cactcggacc atcaccagca gcctcagttg atgacatatc
     5521 tgttgtatcc aatgtccccg gaacatttat ttcagttgct actgggaact ggtcagcagc
     5581 agcaacttgg gacgcaaatg ctgttccaac agctgctgat aatgctatag tttcagctgg
     5641 acatactgtt acagttaatc aatcgggaac aattataaac gccttgactg ttgacggtat
     5701 tttggcttat ggagctacac caacgaattt tacaattaca aataatcttt cagttagctc
     5761 cttaggggtg gttaatgtat ttaacggaac aacaggaaag accttattag tgggtgggaa
     5821 tattgttaac aatggttcta ttgatatttc agttggagga acaaccggtg gaaacctcac
     5881 attaaatgga gcgcaagtgc aaacagtttc aggaacggga acttttgtaa atagtattat
     5941 ccggaactta acttgcagta acacaaatac tgcaactcca aatatcaatt ggctggtaaa
     6001 tgatattaaa atagcttata acctcaatat atccggggca agaattaatc taggaacaaa
     6061 caaactgact tggggaaata atgcagcagg aaataccttc actgctcctg caggtacagg
     6121 ctttcttgcc ggcggaaagt tttcaagatg gtggactact ggacagaccg gttctgcaat
     6181 tacagctggt gctgaaccaa ctactgttac aagcaggtat ccttttatca acgctgctgg
     6241 tcaatctcgt gcgatttata ttagcaggtc ttcatctacc gtaaccggta atgtagcagg
     6301 ggagttggca gttgtttatt cagatgctgc aactgtaaca acgtcactta atattgacga
     6361 tgcaggttac attgtaactg atcgttttga tgggtcatgg ttagtaactg ctgagaacgg
     6421 atatgtttat gtttccggta ctcatacagt tgttccgatt gctacaggcg cttatcctgc
     6481 tgccaacgga aattcacgag tcttgacatc aaatgcagta attggtggta ctcatcagaa
     6541 cggaactagc ggtcctgttg ctcagcgcat tggattatct acagctgaat taactatccc
     6601 aacagggatt tatattggtg ccaacacaac agatattttc caaccatgtt ccggagcacc
     6661 tacttcatcc aactcaattt ccagtgttgc gacggtttgt tcaggagtta actttaatct
     6721 ttcattaagt ataccttata ctgaaacagg gattacctat caatggcaat cctctcccga
     6781 tggtgttaca tatagtaaca tttcaggagc aaccaacgca acctatgtag caacccagac
     6841 ttctgcaaca ttttatcagt gtatcgtaac ctgtgttaat tcaggtcagt ctataactac
     6901 aacccctgtt caggttttgc aaaataattt ccagacttgt tactgtacac caacttattc
     6961 gaacggatgt ggtggtaccg gagctgatgc aattaccaac gtaacacttg gcggtttgaa
     7021 taatagttct ggttgtgcag ccacacccta ttacacctat tacaatgcgg ttactgttcc
     7081 tgatttagtt cgtggcgcta cgatgccttt gtccgtatca tttggtagcg atggaagtca
     7141 gtttgccgga gtgtggattg actttaatca aaatggattg tttagcgatc cgggtgaatt
     7201 tgttgccttt aatacagtta cagcaggcgc aagtggaacg gtgaatttta acattgctat
     7261 tcctgctgga gccacgctcg gtgttacccg aatgagaatc cgcggtggaa atgatactca
     7321 attgttgaat acaccttgtg gagcttcatc ctctacatgg ggtgaaaccg aagattataa
     7381 tgtaaaccta attgatgcga taatttgtgc cggatctccg gttgcagcaa ccactaatgg
     7441 tcccgtatct gtttgtagtg gtgttaactt taaccttaca ctttctgaag actatacttc
     7501 tctttttggt ataacttatc aatggcagtc ttctcctgat ggtatcacat tcagcaatat
     7561 ttcaggggcg accaacgcaa cctattcaac atctcaaaca gctacaactt attaccagtg
     7621 tataattaca tgcggtccat cgggacagtc aaccatatct gttcctgtat tggtaaatac
     7681 ttactctgct tgtttaagca tgccagtatc cggttcactt gcaacctgcg atgccaagtt
     7741 ttacgatagt ggtggtcctg ctggtaacta tggaaataac gaaaacagaa catttacgtt
     7801 tttccccgga acgcccggag gagtggtcgt tgcgacattt gtttcatttg caaccgaagc
     7861 tggttgggat tacctccgca tttacaatgg gaacagcact gctgcacctg ctttgcatac
     7921 gggaagcgga tttaatggag catcgattaa tccaactgta tttacttcta ctgccgcaga
     7981 tggctcattg acatttcagt ttacatctga tggttcaggt acgcccgcag gttgggatgc
     8041 tacaatttct tgtattcttc cccctacaga tcctagcgca cctgtacagg atccggcaat
     8101 gccaagctgt attgcaggta cagatctttc ggttccagga acacctcctg caggggacgc
     8161 atggtattgg cagactgctg caaatggtac aagtacctca agtcctgtaa gcggtatatt
     8221 caccgtgttt caaaatggaa cctattacgt acgttcgttt aacagtacta caggattgtg
     8281 gtcagtaaac agtacttcaa taactgtttc taatattccg gttgcagctg ctcctcctgc
     8341 tcctgttgca gcagcgagtc ctgcatgttt aacaaccact atttctgttg ctgcacctgt
     8401 agatcctaac gtgacttact tctggcaggg aactgtagca aatggtattg ataattccaa
     8461 tgatgctgct agtccattta cagttactgc atcaggaacc tattatgtca gtgcttttga
     8521 tgttagcacc ggttgctggt cagaaggagt tggtgttgct gttctggttg atacctatgt
     8581 tccggatgca ccaacagctg catcaccttt tgatttttgc ataggtgcag cttcagcttc
     8641 aatttcagca actgctccgt cttcttcttc acaaatagca acttcaggaa caatttcaat
     8701 tccaatccct gacaacgatt taaccggaat tagtaatgta cttgcaattt caggtgttcc
     8761 ttcaggagct gttgttgctt ctgttgcagt aaccgttaat attccgcata catttaatgc
     8821 agacctggat atttatttaa caggtccaaa tggaactact attgaactat caactgataa
     8881 tggtggttct tctgataatt atacaaatac tgttttttca aatcttgcca ttaacccaat
     8941 taccggtggt acaggtcctt ttaccggatt gtttttgccc gaaggtgatc tcaccacatt
     9001 gttctcttta cctaatggaa actggacact acgtgtagca gatgatttgg gaggagatct
     9061 aggaaccttg acgagttggt ctgttgaagt cgcctatact gtacctgtaa gtactgttac
     9121 ctggtacgac gctgcttcag gcccttctca aattggaacc gggaacgcac ttgagtcaat
     9181 tggaactacc actttaccca acggaaatac tccgggtact tacagtttct atgctcagtc
     9241 agaaagcgga gcatgctcaa gtttaactcg tgcgcaggtt gatgttgtaa tcaatccctt
     9301 ctctgcaacg gttactgcgg tagatgttac ctgtaacggt gcaaataacg gtacgtttac
     9361 tgttactgct cctaactgcg gaacagctcc ttacgagtat gctgttgacg gtggtgcata
     9421 taccagcacg attcctgcaa accttactcc gggtgcgcat actgtaatgg tgcgtgacga
     9481 caatggtttt gaaaccgcac tttactctat tacaatcaca cagcctgcac aattgatcgc
     9541 atctgaaacg catgttgatg tattgtgtaa cggtgctgca accggatcta ttgatgtaac
     9601 catcaccggg ggaactgctc catatgcgtt cacctggtcg aacggagcaa ttggtgaaga
     9661 ccaaaccgga attgttgcag gaacatacaa ccttgtagtt acagataacc gcggttgtaa
     9721 tgcatttgca agtgtaaata tcactcagcc aactgcagtt gttattgctg aaacacacaa
     9781 taaccttaac tgtaacggcg acgcatctgg atcgattgat ctttctgtaa gcggtggtat
     9841 tgctccttat acgtatagct ggagtaatgg tgctactact gaagatcttt caacacttgc
     9901 tgcaggtgcc tatgttgtta ccgttaccga cgataacgcg tgtacttcaa cgctttctgt
     9961 ttccattact gagcctgcag tgttggctgc tgttgttgca actgcaactc aaccttctgc
    10021 ttgtggtgct acggatggcg cacttgatgt tgctgtaacc ggcggcgtag ctccatatac
    10081 ttttgcatgg agcaatgctg aaacaaccga agacatttct gccatcggag ccggtcttta
    10141 cacgcttact gcagctgatg acaatggatg tttcgttgta cttaacactt ccttgtcaga
    10201 ccccaacgct cctgttgctg ttgttgacct ggttaacaat atactttgct tcggtgcagc
    10261 tacaggtagt attgatgtaa ctgtttctgg tggtgttagt ccttacacct acttctggtc
    10321 aggtggatct acttctgaag acctgagcaa tgctacagca ggtgtttata cacttaccgt
    10381 aactgcagat gatggttgcc agactttcgc aagtgcaaca attactcaga atgcagaaat
    10441 tgttatagct tctgtaacaa catcaacact ttgcaatggc gataacaatg gtgcaatcga
    10501 tatcactgtt accggtggag ttgttgcaac tgcttatgat tacagctgga gcaacagttc
    10561 tatcactgaa gatatcactg cacttgctgc aggaaactac acagtaacaa ttactgatga
    10621 taacctttgt actgcaactt caactattac agttaccgaa cctgctgtac ttgctgctgt
    10681 tggtgtagtt accaatgttt cctgtaacgg attgggtaat ggttcaatca acctcaccat
    10741 tacaggggga actcctgctt acgatgtgtt atggtcgagc ggtgctgtta ccgaagatgt
    10801 tagcggactt gctcctgctt cttacactgt aaccgtaact gatgacaatg cttgtgttgc
    10861 aactgcttcg tttaccataa ctgaacctac tgttcttggt gctactgcaa ccagcacaga
    10921 tgagatcaat ggtaacgacg gaagtgtaaa ccttactgta accggtggat ctacacctta
    10981 ttcatttgct tggaacaaca gcgcaactac cgaagatctt tccggagttg ttgcaggaaa
    11041 ttattctgta acagttacgg atgctaacgg atgtactgct actgtttcca tcactgttgg
    11101 atcacaggtt ggtgtaaatg aacttggtgt tcgtgtaatc aatatctatc cgaatccttc
    11161 caatggtatc ttttatattg agatgaatgg tcagcccgaa agtggtgatc gcatttttgt
    11221 acgtgatgct cttggtcgtc ttgttcttga ggagaacatt atgcaaaacc gcattacgtt
    11281 gaatcttgat acacaagaaa gaggatttta ccttgttgaa gtttggtctg caggacaacg
    11341 ttctgttcac cgtattcaac ttcagcgata agcaaaacag aatatttgaa aaacccccgg
    11401 cagaattgtc gggggttttt tattgaccga actgttcact taacaatgta attggtagtt
    11461 gttcgcttgt tattttcttt ttcagaggct tgttttgtaa aatgcattct accctacatt
    11521 ctcctgaaga aattttgcgg tcatggtttg gttacgacag ttttcgtggt cagcaaaaga
    11581 aaatcattga tacgattgtt gccggtaagg atgcatttgt gttaatgcca acaggcggcg
    11641 gaaaatcact ttgctatcag gttccagcgc tggtcatgcc cggcacttgt ctggttattt
    11701 cgccattgat cgcattgatg aaggatcagg ttcaggcttt acgtattaac ggaattgaag
    11761 ctgcttttct aaattcaagt cttggtgctg aagaggaata ttccatttta cagaagtgcg
    11821 gtgaaggtaa actgaagttg ttgtatactt ctccggaacg agcattattg atgccgcagt
    11881 cgttttggaa aaaggtgaat gtaaatctca ttgcagtaga tgaggcacat tgtgtttcgc
    11941 aatggggaca tgacttcaga aaggaatata cacgtatcgg ggaactgcgt gcaaattttc
    12001 cggatgtctc aatggtggcg cttactgcta ctgccgataa actaacccga aaggatattc
    12061 tcactcatct tggattacat caaccggaag tttttatttc ttcttttgat cgtcccaacc
    12121 tgagcatttc cgttcgctcc ggattgcgtt ctgctgaaaa gaatgatttg ttgttttcat
    12181 ttttagcggc acgacgcaat gatgcgggaa tcatttattg tatctcacga aaaatgtgcg
    12241 aggttttgtc gggaatgctc tttgagcggg gaattcgcaa tgcgttttat catgcaggaa
    12301 tggagtcggt agagcgggca agagtgcagg atgctttttt gcacgatgag atttcggttg
    12361 ttgttgccac tatagccttt ggaatgggta ttgacaaatc gaatgtccgc tgggtggtgc
    12421 attacaattt gccacgtaac atggaaagtt actatcagga aattggacgt gccggtcgtg
    12481 atggtttgcc cggtgacacg ctgttattgt atggtgcacg ggatcttggt atgcttggat
    12541 actttgcaac acaatctgca cagccggaat tgcaattgga gaaattgcac cgcatgcaat
    12601 attttgcaga atccagaatt tgccgcaaac gaaccttgct ttcgtatttc ggagagaaat
    12661 tgacccgtga ttgtggcaat tgcgatgttt gtcttcatcc gccacgcttt tctgatcata
    12721 caaaaacggc acagttggtt tttacactga tcgaacgttg tcgcgaacaa ctatcgcttg
    12781 gtattctggt tggtgtactt cgcggatcga atgcggaaga aatacggtca cgtggttttc
    12841 atttgttgca acagttcggt gccggacgag agcgaccgga gcacgagtgg cgacaggtca
    12901 tctatcagct taccgggatt ggttatttgg aagtggccta cgatgatcat catgcgcttc
    12961 gtttaaatcc ttctgctcaa gaggtgttgg caggaaaggg acgcgtgcag ctggcagaga
    13021 tcccttcgcg gaaaacagaa aggaacagac cggttcctga aatattggtt gcacatcagc
    13081 gggatgcaga ctccggcgaa ctatttgagg cattgcgaaa actgagattg gaaattgcca
    13141 gggaagaaaa ggttgcgccc tatatcgtgt ttcacgataa aagtctaagg gaaatgtgcg
    13201 accgcctgcc gttgagcgaa tcggaattgc gttccatttc cggattgtcg gatcggaaaa
    13261 tggaaaaata cggcgaacgc tttctttctc tgctgcgaga atgggagtcc tttagccaat
    13321 cctgatcaca tcgttttttt cgctgcgaaa tcgtgtttat cttgcagtat gacgaacaga
    13381 cgcagtaacc gactgatcct acttctcttt ttgatgtttt tttacatcct tgcgcaattg
    13441 ggatggtggg cttatttgat ttcacgtctc acacgtgtgg tgcatgccga tcatccgcga
    13501 ttggaacagc gaattttgat ggtttggggt gaaggtttgg tatttgcact tttattattg
    13561 gcaggtttta ttttcaccta tcgttcctat ttgaaagaat tgcggcttgc gcgtattcag
    13621 cgtaattttt tgctttcggt tacccatgaa tttaaaacac ctattgcctc cattcgcctg
    13681 caactggaaa cattattgct tcgaaaggtg gatgaggaaa aacaaaaaca attgcttgaa
    13741 cgcgccatca gggatatcga ccggttaaat ggtttgaccg gaaatattct tactgcttca
    13801 cgcatcgaac aaaatgaatt tcctgtgttt cggtcaaccg cagatctttc tgcattttta
    13861 gttgcttcta tagaacattt gcaaacaaca gttggtaaag atcatactac acaagtaaac
    13921 gtttctcctg ctgtgatttt ttcttttgat gccgatgcaa tgaattccat agtaggaaat
    13981 ctatatgaaa atgctgttaa atattctccg aacggatcaa cgatcagaat agcgctggaa
    14041 aaacgaaatg aacgcatccg gttaacgttt gaagatgagg gaaatggaat ccctgaaaaa
    14101 gagaaactaa aagtatttgc ccgtttcttt cgttcaggtg atgagaatac ccgctctgcc
    14161 aagggaacag gattggggct ttacatcacg cgtcattttg ttttgtcgca caacggaaaa
    14221 atattcatcg agaactgtca gcccaatggt tcgcgttttg ttgttgagtt tgattctaag
    14281 gcataaaaaa aaggacctca tttctgaaat cctttttccg gttttatttt ctgagtatta
    14341 atggcgcaat atggttacag ttccattgta aggaccatat ccttccctct gtggaactac
    14401 aatatagaag taagttccgt cgggtacgtc tccgccattc caggtgttct ggtaattatc
    14461 gctttcaaat actaccaatc cccagcggtt gtaaacttta acggcagtgt tgggatggta
    14521 ttctaaattg gtgatgtacc aaagatcatt cgatccgtca ttgtttggtg agaaaacatt
    14581 cggaataatg atctcacaac caacaaaagt gtaagctgct gtatccattg caatggtgct
    14641 gcactgatcg gttaccataa tgatgtaatc gccattctgc ggattggtaa agaaggttgt
    14701 gcctgcacct tgatcaataa cgaaactggt acctgtcggg cctgtccagc cgaataaatt
    14761 tcctcctgca ccaccgctta caacaggggt tacctgcagt ccttcaccaa gacataattg
    14821 ttcatcggaa atggtgataa ccaatggttg gtaaaccgga acatttacaa taacaggaat
    14881 tatcgtatcg aggttacagt tatcggtaac agtaaaattg tacgttgtag tggtaacggg
    14941 actcacagtg gtagaggttg ttgttcctcc gctgctccag ttgtaagtgt aaggaggata
    15001 accaccgccg tttacggtta cacttaaatt tgcaggatcg cccggacaaa gtgttgtttc
    15061 gtcttgcgag ctgatattcc agggtgtggg tatgggaaca gtgatcagta cagagtcggt
    15121 tccaacaaga ttacagatgt cggttactgt aaggtaatac attgttgtaa ccatgggatt
    15181 cacggttgtg ttttgtgtgg cagcaccgct tgaccacgtg taattgaacg gtggatttcc
    15241 tccggtagca gatgctgcaa gattaacagc ctgccccgga caggtcgggt tttgattggg
    15301 ccctgcttct gcatttatgg gtacaggatc tactatgtaa agttctgcag ttttaattac
    15361 cgtatctccg cagggagtaa tggtgtaaac agtaataatc agtgtgtcgg tggtttcaat
    15421 tacaccatcg gcaattgggt ttacaatgag gttaacagtg tcaacgccaa ccgggaaata
    15481 aacgctgtcg ggcaggaaac tgaaatcgac accattggta gcatttccgg agatggcaat
    15541 tgcaatggta agatcagttg aatcagaagg acggctgaat acaaatgtcg cgtcggaaca
    15601 accttcaatg atagtattgt ttccggtaat ggttgcaact gttacatcaa caccaataga
    15661 agtaagtgat ccgccttcga ggaacacacc cgaatcccaa acaccatcac ctgcatccgc
    15721 aatagcgagt ttgatgtgat aggtttgtcc gcactgcaca gctgtttttg cagttaacac
    15781 aactgtccag ccatcgtact gcacgctgtt ttgggtgttt gtaccggcat aaaaaacatt
    15841 gtaagaggcc catgccggat cgatggagtt gcaatttcct ggagatcctg cagatcccgc
    15901 gactccaaga ttaacggtat taatggaaac cggagtattg gttccgggaa tggttgcaat
    15961 gttttttgca ttgttggtgt aaggacctgt aattccgggg ccgctgatga aaaatccgaa
    16021 agcgtcgttt accgaagcac atacatattc gttgtattct tctgatgcga acacatagcg
    16081 gaacgaaata gaatcaccaa tgggaacaaa gtcaaactcg agaacagcct gatcgtagat
    16141 ctggttaggg gtaatagcgg caagatccgg atcggttgcc atgataccct gcggaggacc
    16201 ctgagaagca cctccgttat tgttaggtcc aaccgcaacc tgtgcgtttc ctgaagcaag
    16261 gataattcct ccggccaaac cgatgttcga actgcttccg ttaaactgac ccgcctgtac
    16321 agttactgta tttccgggag caccgttgaa cgttacgtta gaaaccgtaa cccctgttcc
    16381 aacgagtaca ttctgcacca attgggctgg ggtaagtgtg gtgttgttga caacaagctg
    16441 gctaaatgcg ttagaacagc ttaacaacgt gatggttgca agtaagagct ttttcatgcg
    16501 gttgtattgg gtgtaagtag tagacccaaa ataagcgcat atggttgctt taacctagag
    16561 gcatgttaaa aaaataacca ttcagcggat gagggtataa atccgctgaa tggaaggaat
    16621 gcaagaatga tcgattattt agtcactgta acatgaccgt taacggtggt cggttcagga
    16681 tcccaacact tacgctggta ggtgcagatg taaaaataaa ctccttcgga tgcgggatca
    16741 cctttgatct ttccatccca acgttttatg ctgtcgtcgg attcgaaaac tttcacgccc
    16801 cagcggttgt aaacttccat gtggtaaaca tcaagactgg ctaaagcatc aacgcccgga
    16861 aggttagaat agaacattac gaactgatca ttttttccat cgctattagg ggagaataca
    16921 ttgggaataa caggaacacc ctgagcagcg cttccctgta cgtcaacatt caaggtgatc
    16981 acttcggagc ggttacacaa caagtcgaat gctgtcatgg tgatggtgta attacccgga
    17041 gtggtgtaag tgaaatcgat gagtgtatcg tcaacaaaaa tggttccttc acccatgttc
    17101 caaataatgg aatctgcgcc cgttccggtg aattgtatat tcatgaaaac agaatcggta
    17161 caaggaggaa caggagtgct ggataatgtt gcgttgaaca cttttggttc aagaatagta
    17221 accgacaaat aagcagtatc agcgaggtta catgaagtag gatctattgc gatatacatc
    17281 acattgtaat tacctacatc cacgaaggtg aaattcggat tctgttgtgt tgaagtactg
    17341 ttggcagcat catcaaaatc ccaaaaatgc tgaggggcag ttgaagtact tacaaaactt
    17401 acgttataag gcggatcgca tgcaatgata ttgggagaga catttgcgtt cgcaaaaacg
    17461 gtgtttgcat tgtacactac agtatcggta aacgtttctg atcggttgca gagaaaatcc
    17521 caggctgtta acgttatgat aaatgttccg ggtgtggaat aggtatgcga tacagcgagg
    17581 ttatctgcaa tgatggttcc atcgcccatg ttccacgata gcgaatctgc tccgctaccg
    17641 gtaaactgaa gatctacggt taaaccgccg ggctgacagg gatcgaatgg aggaatatcg
    17701 aattcggctg caaaaattgc acttggtaaa agttcaaccg aaagaaaaac agtgtcggcg
    17761 atattacaag ttgaagaatc gatagcgata tacataacat cgtattgacc aactgcatta
    17821 aatgtgtgaa tcggattttg aattaaagca ttgggagaac catcgccgaa attccagatg
    17881 tgatgtacac ccgaagatga tccctgaaaa tttacattgt agggagcacc acaggcaaca
    17941 atatttggtg cagcatttgc atctgcaacg attcctgtat aactaaattc cattttgatg
    18001 gctccaagat tgcaattggt gcttccgttg ttaggcgcaa ctacacccgg tgttgttgga
    18061 aaagtatttc cacctccgca tgcggcacac acagcctgat aaatggttcc gttcggatca
    18121 aaacgtgatg ttcctccatc aacgtgttca gctgatgttc cgccgaaaaa ggtcccatac
    18181 aattgacttc caccattgcg ttcaagaacg aaaaagtaga aatcgcttcc atcggttgtt
    18241 gcatcgtaag catcggatgt taccggcaaa ccggtggtgg ttccttcgtt gttggtgttt
    18301 ccaccccatc cgctcaggta aacgttttcg caatcatcaa ccagaaatgc cgtgggagaa
    18361 atgttaatgg ttgttcctgt tgtgtttccg attacagtcg agtaaacagt ggtgttgagg
    18421 ttaggagtga gtttatgaat gaattgtttg cttgttccgt tgctgtaaac accgggagtg
    18481 attggatagg ttccttttgt ttgtccggtt acgtaaacat catcgtcatc gtcgagttca
    18541 agaaggtaaa cctgatcgta attgtttgtt ccgatataag ttcctctgat cgatgctcct
    18601 gtatttccat cgagattgaa aatataaccg tcggctgttc ctccgggtgc ggtggcgtgc
    18661 agtgttcctg cgcttacggc aagattggtg cttcgtgttc cgccgcctac atatacactt
    18721 ccgttggagg cgactttaat tgcgtaaccg gcatcggtgc tgcttccgcc aatgtaggtg
    18781 ctccacacaa gtgttgctaa gccggcgttg agtttaaaag cacatccatc ctgtgttccg
    18841 gataatgttt gtgaaagtga tcccgcagtt gtggggaaat tattggatag ggtagaagaa
    18901 gtaacgtaca cattgccggc ggcatctaga atgatctcac cgcgcatctg gtcggcgtaa
    18961 ttaaaattaa gtgttgcagc tgtatttact ccgtcgttgg ctgttcctcc cacaaaggtt
    19021 gaggctagca atgcatttcc tgctgcgttc atctttacaa cgatcatgtc gacaccggcc
    19081 gaataggaga ttccgttgat ggtgatattt gttccgccgc tgaatgaatt atcgtacgat
    19141 cctgctgtta ccggaaaatt actagaactg gttgttccga aaatatgaag ttcacctgcg
    19201 gcatttacca ttagcgaatg cggtgcttca ctgtttgatc ctccaacaaa tgtggaatac
    19261 aagcgggtgt ttcctgttgc agaaaatttg gtaattccaa tatcggttgt tcctccgtta
    19321 aagctgttgt cgtaggctcc tgctgttgtg ggatatcctg ttccgaatgc aacacttccg
    19381 gcgtatagat ttccgctgtt gtcgtaggta gccgtgaatc cccagttgtc tgtagttgat
    19441 ccggtaaaag tggaaaagat caatgttgga tcgatgatca attccaaatt gggattgtaa
    19501 tttcttccca gctgaaaaga aacaattccg tttttgagtt gatagttaca ggatattaat
    19561 tttttctgac catcgatcat ttggtacgcc tccggaatgt attccacaac ttctcct
//
LOCUS       DAOMYG010000006        19352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_979_length_19352_cov_10.546873, whole genome shotgun sequence.
ACCESSION   DAOMYG010000006 DAOMYG010000000
VERSION     DAOMYG010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 19352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19352
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_979_length_19352_cov_10.546873"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1345)
                     /locus_tag="PK610_00450"
     CDS             complement(23..1345)
                     /locus_tag="PK610_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRJ34421.1"
                     /translation="MKKTLFSLFLAGSLTQAIAQQPQKIKFTEYDLPNGLHVILHEDH
                     STPIVTVSVLYHVGSKNEDPTRTGFAHFFEHLLFEGSQNIGRGEYMKLIQENGGVLNA
                     NTSFDRTYYYQILPSNQIELALWMESERMLHAKIDPEGVETQRGVVKEELKQRNENQP
                     YGSILNEIFGHAYSVHPYRWTPGGSPEYINQAKLEEFMDFYRTFYVPNNATLCIAGDI
                     DPKKAKEFIAKYFGDIPKGTRPIPRPKEVEPRKTKEVRDTIFDPNIQLPAVVMGYHIP
                     AQGTPDYYAVSMLGNLLSNGKSSRLYKSVVDEKQKALFAGTFPLPTEDPGLALAFAIA
                     NMGVNAADLEKAIDEVIEAVQKDLISEEEFQKLRNQMESEFINSYSSMEGIAENLANY
                     HVYFKDANLINTELDRYMKVTREDIRRVAKEYFRKDNRVVLYYLPKQQ"
     gene            1497..2690
                     /locus_tag="PK610_00455"
     CDS             1497..2690
                     /locus_tag="PK610_00455"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRJ34422.1"
                     /translation="MNLLSARITNLTESATLAMARKSRELTAKGLDIINLSLGEPDFA
                     TPEFIKDAAKAALDANFTKYTPVNGYQDLRDAIALKFKRDNGIDYTADQIVVSTGAKQ
                     SIANVVMSLVDPGDEVIIPTPFWVSYEEMVKLAEGKCVFIRAGIETDFKITAEQLEKA
                     ITPATKLMIFSTPCNPTGSVYSKKELEGIAAVMERHPRLFVIADEIYEHINFHGKHES
                     LATFASIRDRVITVNGVSKGYAMTGWRIGFIGASKEIADACTKMQGQITSGTCSIAQK
                     AAKAAMLEDPKVTIPMREAFLKRRDLILRLVAEIPGMKCNVPQGAFYIFPEVTDYFGK
                     SHAGFKVQTAGDLCLYLLEHAQVAVVTGEAFGEPRCIRISYATSEDVITEAMRRIKTA
                     LAQLS"
     gene            2690..3688
                     /locus_tag="PK610_00460"
     CDS             2690..3688
                     /locus_tag="PK610_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HRJ34423.1"
                     /translation="MKQIDHLFQQFSGVSALVIGDVMIDAYLWGVVDRISPEAPVPVV
                     NVTRKENRLGGAANVALNIAALGAKPIICSVIGNDRHGNEMDDLIREQGLSAAGLLRS
                     DQRITTVKTRVISHSQQMMRIDEEQTADISKADESGFFERIEKIINEEKPAVIIFEDY
                     NKGVLTESLIARVIELARQKNIPTCVDPKKKNFFSYSGVTLFKPNLKELREGLKLEID
                     RNDDSALLNAVQQLEQRLPHSISLITLSERGVFVKNGATAHRYPAHIRNIADVSGAGD
                     TVIAVAALCLALNADVAVIARLSNLAGGLVCERVGVVPIDKEQLLDEARQEFHNEA"
     gene            3685..5517
                     /locus_tag="PK610_00465"
     CDS             3685..5517
                     /locus_tag="PK610_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRJ34424.1"
                     /translation="MIFQRIREKINLLIYDSKERVLAIFKYLNVIVTLTAVATLVYYY
                     GFPQDTSTIDLSTFILKCSFGFYITHFFIRLFYDFEPGQFLRDHWIEAIMMLVLFIEG
                     LSSILTGKLLLQTFFQSVGFSSFEQFSTIFIQLYFFFVVLIGLTRTYTIMPFIRLHPA
                     TLFLLTFLLLIISGTGLLMLPEMTVQEGSMSFIDALFTSTSASCVTGLSVVDVSTYFT
                     LKGQMVILMLIKVGGLNIVGFVAFIILMSKFGIGVKYHSFMDDYAKRGTIESALRMLR
                     KILVWSLFFELSGALLFYFFLGDDNPLTPTGSDRFFASVFHSVSAFNNAGFSIFQDGM
                     FNEFVRHNYFLHIVLMVLIFLGSLGFMAIFDLFSVENVRDRMRNPWKRINFNTKIALN
                     FSIILVLAGFVMFFMFEYYHTLNDKNSGEKVVTALFQSVTTRTAGFNTVDIGSLSTPT
                     LIFFMFLMFVGASSNSTGGGIKTSTFAILWASTVATMRERKNVELFKRTISSETVLKA
                     FTILLFFIAWNLIAILMLMVTEQDAIYYGQHTELDVIFEQVSAFGTVGLSTGVTSSLT
                     VAGKIIITLSMFTGKIGALTLAYLFGKKALSTNYKYPKANALVG"
     gene            5501..6472
                     /locus_tag="PK610_00470"
     CDS             5501..6472
                     /locus_tag="PK610_00470"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HRJ34425.1"
                     /translation="MLWWVNFGNRHLVPAAIAAVILIFSSTVSFGTPAVYSAIEEPDA
                     DSLSINKKRLRTVLIAEGVSYASTMTALYFVWYKDQPLVKLHSFDDADEWLQMDKAGH
                     FVTGYLIASYSDRFYRYANMSPKKAALIGSAQSLFFLSSLEMFDGISRDWGFSWSDMG
                     ANVLGCGSYLLQEYLWREQRIIPKFSAHTTHLAAYRPELLGSTFPSRILKDYNGQTYW
                     LSFNLASTFGLQKSNLPKWLNIAFGYGANGLLGGTINPERNKAGELLPHKERTRQYYF
                     SLDIDCSRIPAKKKWQKVVLRSLNWIKIPFPTLVLEQGKFELLPFYF"
     gene            complement(6475..7032)
                     /locus_tag="PK610_00475"
     CDS             complement(6475..7032)
                     /locus_tag="PK610_00475"
                     /inference="COORDINATES: protein motif:HMM:NF027596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4924 family protein"
                     /protein_id="HRJ34426.1"
                     /translation="MRAGEEKLKKNIAEYILYIWQMEDLVRSFQFDANVIRQSILSPQ
                     IEDEKELELEMEWFNELIRKMKNQQLVQKGHLNELNEIIQELFYLHNTLLNVAKDQAY
                     VALNDAAAEAMEEFRFKSKLNDMNTVELCLNGLYLKLLMRLSKQEITSATETAFDSFR
                     KLIAYLTIQYHKMKNGDLQFNYNLN"
     gene            complement(7036..7689)
                     /locus_tag="PK610_00480"
     CDS             complement(7036..7689)
                     /locus_tag="PK610_00480"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRJ34427.1"
                     /translation="MNSQKLSLTLNVVLLIAVLYLFFKVSSLSSAVSGHHGETSVNSG
                     NNPKDTGVKVNRVHEEDGQLVIRYVDIDSLNSQYELLKDAEKEFQALNKQAEAQYKGK
                     IQAAQKRAQEIEKEFQFMTLSQQKAAQEEMARMEKDIQMSEQRIFGPVQDKFTVRKNE
                     IDRTVKNYLREYCVKNNIDFILGQMSAVNFLMYSNPSMDITEEIIAGLNAEYAESKK"
     gene            complement(7693..7938)
                     /locus_tag="PK610_00485"
     CDS             complement(7693..7938)
                     /locus_tag="PK610_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34428.1"
                     /translation="MRVIAEIPHPQIKITIFSWNAKYIIKLEAGPYEQTFKIAESDIA
                     GLEQVQALLTPEFIDACIQRFIAMRSDFSTSFKSLNV"
     gene            complement(8034..9107)
                     /gene="serC"
                     /locus_tag="PK610_00490"
     CDS             complement(8034..9107)
                     /gene="serC"
                     /locus_tag="PK610_00490"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRJ34429.1"
                     /translation="MKKVHNFSAGPGILPAEVMKQASEACINFNDLNLSLLEISHRSK
                     DFEAVMDEARNTIRELFGVNEDYSVLYLGGGASLQFGMIPYNLLRSNGKAAYLNTGVW
                     ATKAIKEAKMLGDTVVVASSEDKNFNYIPKNYTIPADADYFHLTSNNTIYGTQIKQFP
                     NSPVPMVCDMSSDIFSRKVDGNKFGMIYAGAQKNMGPAGTTMVMIHKDLVGKSDRKKL
                     SMMDYAVHIKGESMYNTPPVFPIYVTLLTLRWLKKNGGVQWIEKVNQEKADLLYNEID
                     RNSLFYGTAEKEDRSTMNICFLLHKTELEPEFDKLWKEANISGIRGHRDVGGYRASTY
                     NAMPIESVKALVEVMQAFEKKFA"
     gene            complement(9207..10082)
                     /locus_tag="PK610_00495"
     CDS             complement(9207..10082)
                     /locus_tag="PK610_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34430.1"
                     /translation="MKRILIVLFLITGLPSLHAQYELEQMRFQEDSLRYCLDKLRSSQ
                     KDADRKIWNKKFKLMLEETLDMKGAFEYPFDSLKSIARIYSPDKVFRLFNWNVENDNG
                     TFTYYGFVMVPGNKTSLFELVDQTPVIHDPEDKTLDNKKWLGALYYDIILSGTTTRKE
                     YTLLGWDGYSRMSQRKIIDVMIIQADKIQFGAPIFMAGPKGGWKKRVVFEFSSKAKMT
                     MKYSVKDQMIIFDHLIPESEMAEGIREFYVPDGSYDGYALVNERWEYRSDVDARRPKD
                     KKDRFWNDPKKTGNN"
     gene            complement(10178..10525)
                     /locus_tag="PK610_00500"
     CDS             complement(10178..10525)
                     /locus_tag="PK610_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRJ34431.1"
                     /translation="MAIMITDECINCGACEPECPNNAIYEGGAEWKFADGTSLKGMVT
                     KPGGGAVSADDNHAPKTMDVYYIVTDKCTECVGFHDEPQCAAVCPVDCCVDDPEHRES
                     KEQLLAKKDFMHL"
     gene            10594..11652
                     /locus_tag="PK610_00505"
     CDS             10594..11652
                     /locus_tag="PK610_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HRJ34432.1"
                     /translation="MDLKQRIAAFVQLGKFLSDFAEAKEWQGYEHGVSKSDFEAFTAL
                     IHRVHHHNGWFSEEMVRKALGDWSGLLTEKNLEKWTSAYPELKNTGGNKRVALIMAGN
                     IPLVGFHDLLSVLISGNSALVKLSSDDDLLIPFLTDYLIQHIEPGFSGKVNYSKGKLE
                     HFDAVIATGSDNSSRYFEHYFSSWPHIIRKNRTSVAVLSGLETEEQLKSLGHDIFDYY
                     GLGCRSISKVFIPRDFDLDRLFGAIYSFKDVIHHKKYANNFDYHKALYLMNRDQLLEN
                     GFLLVKEDEGLHSPLGTLFVERYVSYDEVENKLAGLEEKIQCRVGTGGIPFGDAQRPA
                     LWDYADGVDTMKFLIGLQ"
     gene            11652..12302
                     /gene="pdxH"
                     /locus_tag="PK610_00510"
     CDS             11652..12302
                     /gene="pdxH"
                     /locus_tag="PK610_00510"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HRJ34433.1"
                     /translation="MDTLRNYINSIRRDFANRPLDESSVPADPYYLFERWFDEAVGAQ
                     VLDPYAMIIATAGLDGQPTSRVVYMRDISKSGLVFYTNFESNKGKNLIENPKASALFF
                     WGDLDRQIRVEGRVVKADDELSDAYFSKRPRESQIGAWASAQSSVLLSRAELEQKVEQ
                     LTREFEGKDIPRPPFWGGFLLMPDSFEFWQGRPSRLHDRILFTKAGEAWQTSRLSP"
     gene            complement(12533..>12758)
                     /locus_tag="PK610_00515"
     CDS             complement(12533..>12758)
                     /locus_tag="PK610_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34434.1"
                     /translation="AAAKKKAAPKKKAAAKKKAAPKKKAAAKKKTATKKKAAPKKKAA
                     TKKKAAPKKKAAAKKKAAPRKKAAAAPAAN"
     assembly_gap    12759..12858
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13045..13734)
                     /locus_tag="PK610_00520"
     CDS             complement(13045..13734)
                     /locus_tag="PK610_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRJ34435.1"
                     /translation="MDQELIVPYIEFLSQFITSDRLQRFEEVLSNRTRHITMVIEDIY
                     QPQNASAVVRTSECLGLQDIHIIEQNNVYELNKDVVVGASKWIRMHKHKKNPNNTRTC
                     YERLRAAGYKIYATTPNINGYTPDTLPLDEKVAIVLGNEGEGLSATAIEEADGCIQIP
                     MYGFTESYNISVAASIIAYELVQRLHASEIEWQLNEDEKNEIRLRWMKKVLRRVELFE
                     SEFYTRRGGKK"
     gene            13827..16040
                     /locus_tag="PK610_00525"
     CDS             13827..16040
                     /locus_tag="PK610_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HRJ34436.1"
                     /translation="MKRFLFLLLFSVLQMLQSVNAQMAITSESWDSRRMREMNVQQMA
                     VQNSYEVLAIAVDATIRDQIAEVSVTQTIYNPGNQALEVEIFFPLPNGGIVQNFMMMV
                     NGKEIPGQLLKQEEARGIYESIVRRKRDPALMEYVGYGLFKTSVFPIQVGEERNITVR
                     YTQVCDRNLNAVNFSYPFGTQKFSAKALRSLSFNARIESSDGIKTVFSPTDDIIIKRT
                     GDKTAHIKMEKTYVLPTSDFKMVFTTDKGEVGASLLSYKEASDKDGYFMLLASPSVDE
                     IVKQSEKTVLFVLDRSGSMSGQKIEQAKKAVEFVMRNLNDNDLFNIVSYDDRIETYKP
                     ELVRYNKKAYEECYTYINGIRPGGGTNINGALVAGMEMLHDDKRPTYVIFLTDGLPTA
                     GATDELTIANNCKNANKVKARLFAFGVGNDVNARLLDRLSSANGGLSEYVKPAEDIEA
                     SVARLYSNISSPVLTDIKISFEAVEVFMPVSGLDIRSTYPEIMPDLFRGGQIVWVGKY
                     NKPCKANVVIRGKSNGVNKEYRFPVEFVSHTGTKTHDYVEKIWASRRIGHLINQIDLN
                     GRNTELVDELVRLSKEYGILTPYTAFLAREDVDLNAMSQNQRIADEELKELEVVRGES
                     ANDQRAYKSTMQNNTKVQNSAPVQGGAYKKDEQAEKNMANVRNVGSKTFYNKNNQWVD
                     GSLDAKTAKNVVKVRMYSDEYFKLAKGQSAEFNQYLSVGENVVVVINNVAYEFYK"
     gene            complement(16104..19340)
                     /locus_tag="PK610_00530"
     CDS             complement(16104..19340)
                     /locus_tag="PK610_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ34437.1"
                     /translation="MRLYSIHVCILICSLFSTSIAFAQIPSNVPQAGLVAWYNLDNSP
                     VCEVTPTLNGVFSGTVAGMQDRFGNNHKAVDFTGTGKMDVTGINITSGPRSYSFWFRH
                     NSSQQNILLNWSGGLELRTTSSGQLQIQQATPNINATLPNFPQNQWNHIVVTFGSGIL
                     KVFLNGSNIYSLPQTLNNWNDFTMGYGGLPCIGMFDELGIWAFELNQSQIDALYQRGG
                     CLKAEYKFSGNVNDGGGFSQHGTATDITYVNDRFGVPQSAVQFNGSSSFITGPTWYWP
                     AGYNQRSIELWFNVSDTIYNGYHRLFHYGGTGTGSSQEILPTTNALGVMNTLTYSGGN
                     DNVIVGFNGVYNQWHHVVATYDGSVAKLYINGQYINQEPKSWPTQMTTNFIFGAIVDF
                     THFYKGKLDDVRVYGCVLNNSQVDSLYNLPAGGSPCQITASASTVSPSCFQGSNGSID
                     LSLSGGTSPYTYMWSNGSTTQDLSTITAGNYTVVIHDATGCDAMLTVNVPHPTSMSLN
                     VSTTSPTCGMDNGSASVAVSNGVMPYTYNWSNGDNISVADSMTAGTYMITAYDANGCS
                     AAHTFMLSSSGAPTVSSVVTNATCAGSNNGAIDLTVTGGTPPFSFVWSNSSTTEDLAG
                     IKPGSYMVQITDAAGCITGAGIIVGVSSIQVGTPVISQPACGASDGSIAMNPTGASPF
                     TYLWSANAASATTSSVSGLPSGIYSVTISDNNNCSVTQNISLSNPNAPVTQLVSVMPA
                     NCSGMLGAINMNVTSGTPPFTYSWSNGATTQDISNLLQGTYHLSVTGGNGCMSHSSAT
                     VPGIFPDPITLCMSTVDSASGKVLCVWEKPNLFNIDRFNIYRENAVAGQYDFWFTKPF
                     DSLSQWTDQSANPVIRGWRYKVTMVDTCGNESSFGTNHKTLHLVSSRGLSGEVNLIWD
                     TYEGFNYPTVYINRYHASTGWELIDSVPSTLHAYVDVTAPASGLLRYTVDVRPPTSCE
                     ATRAINHNSTRSNRGTIAPPAAVDELLQSSFRVFPNPTDGVVSIVGSDPSAFIQNVMI
                     YDLSGKRIHRMNIRSSATSISIENLERGIYILEVHTPTGIARYRIVRS"
BASE COUNT         5109 a         4028 c         4322 g         5793 t
ORIGIN      
        1 tcatagtcgt tctgttgaat gattattgtt gcttgggaag ataatacaac acaaccctgt
       61 tgtccttgcg gaagtattct tttgcaacgc gacgaatatc ttcgcgggtt accttcatgt
      121 agcgatccaa ttctgtattg atgagattgg catctttaaa ataaacatgg tagttagcca
      181 gattttcggc aatgccttcc atgctcgagt aggaattgat gaattcactt tccatctggt
      241 tgcgcagttt ctggaattct tcttccgaga tcaaatcttt ttgaactgct tcaattactt
      301 catcgattgc tttttcaaga tcagcagcat ttactcccat attggcaatg gcaaaagcca
      361 atgctaatcc cggatcttcg gtgggaagag ggaaggttcc tgcaaacaat gctttttgtt
      421 tttcgtcgac caccgattta tacaggcgag aactttttcc gttggaaagc agattgccca
      481 acatggaaac cgcataataa tccggtgtgc cctgtgcagg gatatgataa cccatcacta
      541 cagcgggtaa ctggatgttc ggatcaaaaa tcgtatcgcg tacctctttc gttttgcgtg
      601 gttctacttc tttgggacgt ggaatgggac gtgttccttt tggaatgtcg ccaaaatatt
      661 tcgcgataaa ttcttttgct tttttaggat caatatcgcc tgcaatgcaa agtgttgcgt
      721 tgttgggaac ataaaacgtg cggtaaaaat ccatgaattc ttccagtttc gcctggttga
      781 tgtattcagg agaaccaccg ggagtccagc ggtaaggatg cactgaatag gcgtgaccaa
      841 aaatttcatt caggatcgaa ccataaggct ggttttcgtt ccgttgtttc agctcttcct
      901 ttaccacgcc gcgctgtgtt tctacacctt caggatcaat tttcgcgtga agcattcgtt
      961 ccgattccat ccataaagcc agttcaatct ggttggaagg cagaatctgg taataatacg
     1021 tacggtcgaa ggaagtattg gcattcaaaa ctcctccgtt ttcctgaatc agtttcatgt
     1081 attcgccgcg accaatgttt tgtgatcctt caaacagcaa atgctcaaag aagtgcgcaa
     1141 aaccggtgcg agtggggtct tcgttttttg atcctacatg gtagaggact gaaacggtga
     1201 caattggcgt tgagtggtcc tcatgcagaa ttacatgaag tccgttggga agatcatatt
     1261 ccgtgaattt gatcttttgt ggctgctgag cgatagcctg tgtaaggcta ccggcaagaa
     1321 acaggctgaa aagggttttt ttcataaatc agatttgaca acacaaaaat aaaagttctg
     1381 gattaccgga gaagggaagc tacaccagcg ggaaatgcac gtgatgaccg gatagtgcta
     1441 ctgtttttcg ctcattttct tcgtgatggt cggcaaagca tatatatttg taacgtatga
     1501 atctactctc cgcacggatc accaacctga ccgaatcggc aacgctggcg atggccagga
     1561 aaagcaggga gctcactgcc aaggggctcg atatcattaa cctgagtttg ggtgaacccg
     1621 attttgctac accggaattc atcaaggatg cagcaaaggc agcgctggat gcaaacttta
     1681 ccaaatacac tccggtaaat ggataccagg atttacgtga tgcgatcgca ttaaaattta
     1741 agcgggataa cggcatcgat tatactgctg atcagattgt tgtttctact ggtgcaaagc
     1801 agagtattgc aaatgtggta atgagtctgg ttgatcccgg agatgaagtc atcattccca
     1861 ctccgttttg ggtttcttac gaagaaatgg taaaactcgc tgagggtaaa tgtgttttta
     1921 tccgtgcagg tattgaaacg gattttaaaa tcacagccga acaacttgaa aaagcaatta
     1981 cacccgcaac caaactgatg attttttcta cgccatgtaa tccaaccgga tcggtgtatt
     2041 cgaaaaaaga actggaaggc attgcagcag tgatggaacg ccacccgcgt ttgtttgtta
     2101 ttgctgatga aatttatgag catattaatt ttcatggcaa acatgaaagt ctcgccacct
     2161 ttgcaagtat tcgcgacaga gtgatcacgg taaatggtgt atcgaaaggt tatgcgatga
     2221 caggctggag aattggtttt attggtgcgt cgaaagaaat tgcagatgcc tgtacaaaaa
     2281 tgcagggaca aatcacttcc ggtacctgtt caattgcaca gaaagcagca aaggcagcaa
     2341 tgctcgaaga tccgaaagtc actattccga tgcgtgaagc atttttaaaa cgccgggacc
     2401 tgattcttcg tcttgttgct gagattcccg gaatgaaatg taatgttccg caaggagctt
     2461 tttatatttt tcctgaagta accgattact ttggtaaatc gcatgcaggc tttaaagtgc
     2521 aaacggcagg tgatctctgt ttgtatttgc tcgaacatgc acaggttgcg gttgtaaccg
     2581 gtgaagcatt tggcgaacct cgttgtatcc gtatttcgta cgctacttcc gaagatgtaa
     2641 ttacagaagc gatgcgtcgc attaaaacgg ccttggcaca actatcctga tgaaacaaat
     2701 cgatcatctc tttcagcaat tttccggtgt ttcggcattg gtaatcggcg atgttatgat
     2761 cgatgcatac ctctggggtg tggtagaccg tatttcaccc gaagcgcctg ttcctgttgt
     2821 taatgtaacg cgtaaagaaa atcgtctcgg cggggcagca aatgtggcct tgaatattgc
     2881 agcactcgga gcaaaaccca ttatctgttc ggtgatcgga aatgaccgtc atggaaatga
     2941 aatggacgat ctgatccgcg aacaaggtct ttctgctgca ggtctgcttc gttcagatca
     3001 acgtatcact accgttaaaa cgcgtgtcat ttcgcattcg cagcaaatga tgcgcattga
     3061 tgaagaacaa actgcagata tttcgaaagc ggatgaatcc ggattttttg aaaggattga
     3121 aaaaatcata aacgaggaaa aacctgctgt catcattttt gaagattaca acaagggcgt
     3181 ccttaccgaa tcgctcattg cacgggtaat tgaactggcc cggcagaaaa atattcctac
     3241 ttgtgttgat ccgaagaaaa agaatttctt ttcgtattcg ggtgttacct tatttaaacc
     3301 caatctgaag gaattacgag aaggattgaa attggaaata gaccgcaacg atgattccgc
     3361 attgctgaat gccgttcagc aactcgaaca gcgtttgcct cattccattt cattgatcac
     3421 tttaagtgaa cgtggtgtat tcgtaaagaa tggagctact gctcatcgtt accctgctca
     3481 tatcagaaat attgcagatg tgtcgggagc cggagatact gtaattgctg ttgctgcact
     3541 ttgtctggcg ctgaatgcag atgttgcagt tattgcccgc ttatcgaatc tcgcaggtgg
     3601 attggtctgc gaaagggtgg gtgtggttcc aatcgataaa gaacaactgc tcgacgaagc
     3661 ccgtcaggag tttcataacg aagcatgatt ttccagagga tacgcgaaaa gatcaatctc
     3721 cttatttacg acagtaaaga aagggtactt gctattttta aatacctcaa tgtaattgtc
     3781 accctcaccg cagtggccac gctggtgtat tattacggtt ttccgcagga tacttctacc
     3841 atcgatctga gcaccttcat tctgaaatgt tcatttggtt tttatatcac gcattttttt
     3901 atccggctct tctacgattt tgaacccggc caatttttac gcgatcactg gatagaagcc
     3961 atcatgatgc ttgtcctctt catagaggga ttgtcgagta ttctcacagg taagctgcta
     4021 ttacaaacct tctttcaaag tgttggcttc agttcgtttg aacaattcag tactattttt
     4081 attcagctgt atttcttttt tgtggtgctc atcggactaa caagaactta caccataatg
     4141 ccctttatcc gtttgcatcc tgcaacattg tttctgctca cctttttatt gctcatcatc
     4201 tcggggacag gacttctgat gctgcctgaa atgacggtgc aggaaggttc aatgtccttt
     4261 attgatgcct tgttcacctc taccagtgca agctgtgtaa caggattatc cgttgtagat
     4321 gtttccacgt atttcacact caaaggacaa atggtcattt tgatgctgat caaagtggga
     4381 ggattgaaca ttgtgggatt tgtcgccttt atcattttga tgtcgaagtt tggtattggt
     4441 gtgaaatacc attcgtttat ggacgattac gccaagcgcg gaaccattga gagtgcattg
     4501 cgcatgctgc gcaaaatttt ggtttggagt ttgttttttg agttatcggg tgccttgctg
     4561 ttttatttct ttttaggtga tgataatcct ttaacaccta caggcagcga tcgttttttt
     4621 gcatctgttt tccattcggt ttcagcattt aacaatgcag gattttccat tttccaggat
     4681 ggaatgttca atgagtttgt acggcataat tatttcctgc atattgtttt aatggtactc
     4741 atctttttgg gctcactcgg atttatggcc atattcgatt tgttctctgt agaaaatgtc
     4801 agagaccgaa tgcgcaatcc ctggaagagg atcaatttca atacgaagat cgcattgaat
     4861 ttttccatca tattagtgct cgccggattt gtgatgtttt ttatgtttga gtattaccac
     4921 acactaaatg ataagaacag cggagaaaaa gttgtcactg ccctttttca atcggtcacc
     4981 acccgtactg ccggattcaa taccgtagat attggttcac tgagcactcc cacgttgatc
     5041 ttttttatgt ttctcatgtt tgtcggagca tcaagcaatt caacgggtgg tggtatcaaa
     5101 acttcaacct ttgctattct ttgggcatca accgttgcaa caatgcgcga acgtaagaat
     5161 gtagaattgt ttaaacggac catcagttca gaaacagtat taaaagcatt taccatatta
     5221 ttgttcttca tcgcatggaa ccttattgcc attcttatgt tgatggttac cgaacaggat
     5281 gcgatctatt acggacaaca caccgaactg gatgtcattt ttgaacaagt atctgcattt
     5341 ggaacggtag gattatcaac cggtgtaact tcttctttaa cggttgccgg aaaaatcatt
     5401 atcactttat ccatgtttac cggaaagatc ggtgcgctca cattggcata cctctttggg
     5461 aaaaaagcgc tcagtaccaa ttataaatat cccaaggcca atgctttggt gggttaattt
     5521 cggaaaccga catcttgtac ctgctgccat cgctgctgta attttgattt tcagcagtac
     5581 tgtttctttc ggaactcccg ctgtttattc cgctatcgaa gaaccggatg cagattcgct
     5641 ctccatcaat aaaaaacgat tgcgtactgt tctcattgct gaaggagtta gctatgcatc
     5701 tacaatgacc gctttgtatt ttgtgtggta caaagatcaa ccattggtga aactgcattc
     5761 gtttgatgat gccgatgagt ggctgcaaat ggataaggca ggacattttg taacggggta
     5821 tctgattgct tcctattccg atcgctttta tcgttatgcg aatatgagtc cgaagaaagc
     5881 tgcattgatc ggatcggcgc aaagtctttt tttcctcagt tcgctggaaa tgtttgatgg
     5941 aatttcccgc gactggggat tttcctggtc ggatatggga gccaacgtgc tgggttgcgg
     6001 atcgtatctt ttgcaggaat atttgtggcg tgaacagcga atcataccca aattttcggc
     6061 ccatactaca caccttgcag cttatcgtcc ggaattgctg ggctcaactt ttccttcccg
     6121 cattttgaaa gattataatg gtcagacgta ttggttatcc ttcaacctgg cttcaacatt
     6181 cggattgcaa aaaagtaatt taccgaagtg gttgaacatt gcatttggtt acggtgccaa
     6241 tggattgctg ggagggacca taaatccgga acgcaacaaa gccggcgaat tattacctca
     6301 taaagaaaga acccggcaat attatttctc actggatatt gactgcagtc gtattcccgc
     6361 aaaaaagaaa tggcagaagg tagttttgcg atcgctgaac tggattaaga ttccttttcc
     6421 gactcttgtg cttgagcagg ggaaatttga attactgcct ttttatttct gagttcagtt
     6481 cagattgtaa ttgaactgca gatcgccatt tttcatttta tggtactgaa tggtgaggta
     6541 ggcaatcagt tttctgaacg aatcgaatgc agtctccgtt gcggaggtga tttcctgttt
     6601 gctcaggcgc attaaaagtt tcaggtaaag cccgttcaaa caaagctcaa cggtattcat
     6661 gtcattgagt ttgcttttga aacggaattc ctccatggct tcggccgcag catcgttcaa
     6721 tgctacataa gcctgatctt ttgcaacgtt aagcagggtg ttgtgcaggt agaacaattc
     6781 ctgaatgatc tcgttgagtt cgttgaggtg acctttttga accaattgct gattcttcat
     6841 tttgcggatg agctcattga accattccat ttccagttca agctcttttt cgtcttcgat
     6901 ctgtggcgat aaaatgcttt gacgaataac gtttgcatca aactgaaaag aacggacaag
     6961 gtcctccatt tgccagatgt acaggatgta ttcggctatg ttttttttca gtttttcttc
     7021 tccggcacgc atgatttatt ttttgctttc tgcgtattcg gcatttagtc cggcaatgat
     7081 ctcttcggtg atatccatgg atggattgga atacatcagg aagtttacgg cactcatctg
     7141 acccaaaata aaatcgatgt tgttcttcac gcaatattcg cgcaggtagt tttttactgt
     7201 gcggtcgatt tcatttttac gcaccgtgaa tttatcctgc accggaccaa aaatgcgttg
     7261 ctccgacatc tgaatgtctt tttccatgcg ggccatttct tcctgtgcag ctttctgctg
     7321 ggagagggtc atgaactgaa attccttttc aatttcctgc gcacgttttt gtgcggcctg
     7381 aatttttcct ttgtattgtg cctctgcctg cttgttcaat gcctggaatt ctttttccgc
     7441 atcctttagc aattcatact gactattcag tgaatcgata tcgacgtaac gaataaccag
     7501 ctgaccatct tcttcatgaa ccctgtttac ctttacaccg gtatctttag gattgtttcc
     7561 ggagttgacc gatgtttcgc cgtgatgtcc ggatactgca gagctcaggg aagaaacttt
     7621 aaagaaaagg taaagcaccg cgattaggag tactacgtta agcgtaagtg ataatttttg
     7681 tgagttcatc gtttatacat tcaaggattt aaagctagta gaaaaatcgg agcgcatggc
     7741 gataaagcgt tgaatacatg cgtctataaa ttctggagtt aataatgcct gaacctgttc
     7801 cagtccggca atatcggatt ctgcaatttt aaatgtttgt tcatagggac cggcttccag
     7861 cttaatgatg tatttcgcat tccagctgaa aatggtgatt ttaatctgtg gatgaggtat
     7921 ttctgcaata acgcgcattg ggcaaaagta cacaacggca cggcattaca gcgttaaaaa
     7981 agcaaaaaac cggtcaatat tgaccggttt ttcgaaaaaa taacggattt gtatcaggca
     8041 aacttctttt caaatgcctg catcacttca accaaagctt ttacactttc aatcggcata
     8101 gcattgtagg tcgaagccct gtaaccgcca acatcgcggt gaccacgaat tccgctgatg
     8161 tttgcttctt tccagagttt gtcgaattca ggttcaagct cagttttgtg caggaggaaa
     8221 cagatgttca tggtcgaacg atcttctttc tctgctgttc cgtaaaacaa actgttgcgg
     8281 tcaatttcgt tgtaaagtaa atccgctttt tcctgattca ctttttcgat ccactgcaca
     8341 ccaccgtttt tctttaacca acgcagggta agcagggtaa cataaatagg aaatacagga
     8401 ggagtattgt acatggattc gcctttgata tgtacagcat aatccatcat gcttaatttc
     8461 ttgcgatcgc tttttccaac caaatctttg tgaatcatca ccatggtagt tccggcagga
     8521 cccatatttt tttgcgctcc ggcatagatc attccgaatt tgtttccatc cactttacgt
     8581 gagaaaatat cggaagacat atcgcaaacc attggtacag gactgtttgg gaattgtttg
     8641 atttgtgtac cgtaaatagt gttgttggaa gttaaatgga aataatctgc gtcggcagga
     8701 atggtgtagt ttttcggaat gtagttgaag tttttgtctt cggaagaagc tacaacaaca
     8761 gtatcgccta acatttttgc ttctttaatg gctttagttg cccatacacc ggtattgagg
     8821 tacgctgctt ttccgttgct gcggagaagg ttgtagggga tcataccgaa ctgaagactt
     8881 gcaccgccac cgaggtagag cacagaataa tcttcattaa caccgaataa ttcgcgaatg
     8941 gtatttcgtg cttcgtccat taccgcttca aaatctttac tgcggtgaga aatctccagc
     9001 aatgaaagat tgagatcgtt aaaattgata caggcttcgg atgcctgctt catcacctct
     9061 gcaggtagga tacccgggcc tgcgctaaag ttgtggactt ttttcatgtt cggaagcttt
     9121 ggttgatagc tgacacaaaa ttggaaatat tccgcctcat aaaaaaatga aaaaggaggg
     9181 gaatgccctc ctgattgtaa taatcgttaa ttatttcccg tttttttcgg atcgttccaa
     9241 aaccggtctt ttttatcctt cggccttcgg gcatcaacat ccgagcggta ttcccagcgc
     9301 tcattcacca aggcataacc atcgtacgat ccgtccggaa cataaaattc ccttattcct
     9361 tctgccattt ccgactcagg tatgaggtga tcgaatatga tcatctggtc tttaaccgaa
     9421 tatttcatgg tcatttttgc cttgctgctg aactcaaata ccactcgttt tttccaaccg
     9481 ccttttggtc ccgccataaa tattggagca ccaaactgaa ttttatcggc ttgaataatc
     9541 atcacatcaa tgatcttgcg ctgactcatt ctgctgtatc cgtcccaacc gagcaaggtg
     9601 tattcttttc tggttgttgt tccggataag atgatatcgt aatacaatgc gcctaaccat
     9661 tttttattgt cgagggtttt gtcctccgga tcgtgaatga ccggtgtttg atcgacaagt
     9721 tcaaacaggc tggttttatt acccggaacc atcacaaatc cgtaataggt aaaggttccg
     9781 ttatcatttt caacgttcca gttgaacagt cggaatactt tatcgggcga ataaatacgt
     9841 gcaatggatt tcagcgaatc aaaaggatat tcgaatgcgc ctttcatatc aagcgtttct
     9901 tccagcatca gtttgaactt tttgttccag atcttacggt ctgcatcttt ctgcgaactg
     9961 cggagtttgt ccagacaata ccttagcgaa tcttcctgaa accgcatttg ctccaactcg
    10021 tactgtgcat gcagcgaggg gaggccggtg atcaaaaaga gaactatcag aattcttttc
    10081 atagcttgtt tactgcgaat ttcgtgccgc tttccggatt tccggaaaag aaaaaacacc
    10141 gtttcataac ggtgtttttc caataaggtt acctctatca aaggtgcata aagtcttttt
    10201 tcgccagaag ctgctctttc gattcgcgat gctccggatc atcaacgcaa cagtctacag
    10261 gacaaaccgc tgcacattgt ggttcgtcat gaaaaccaac acattctgtg catttatcgg
    10321 ttacgatata gtacacatcc atggttttgg gtgcgtgatt gtcgtctgca cttactgctc
    10381 ctccgccggg tttggtcacc attcccttta atgaggttcc gtctgcaaat ttccattctg
    10441 ctccgccttc gtatatcgcg ttgttcggac attcaggttc gcatgcaccg caattgatgc
    10501 attcgtctgt gatcatgatg gccatgtcgg ttgtgttttg ttttgtttta gttttgcaaa
    10561 tatagaaata gcatcatttc tgagttgcaa ataatggatt taaaacaacg aatcgccgca
    10621 tttgttcagt tgggaaaatt cctgtccgac ttcgctgaag ccaaggaatg gcaaggatat
    10681 gagcatggtg tgagcaagtc cgattttgag gcatttactg ctctcattca ccgcgttcat
    10741 caccataatg gttggtttag cgaagaaatg gttcgcaaag cactcggcga ttggtcggga
    10801 ttgctcacgg aaaaaaatct ggaaaaatgg acatcggcct atccggaatt gaaaaatacg
    10861 ggtggaaaca aacgcgttgc actcatcatg gccggaaata ttcctttggt cggatttcat
    10921 gatctgctca gtgttttgat cagcggaaat tctgccttgg tgaaattatc gtcggatgat
    10981 gatctgctca ttccttttct taccgattac ctgatccaac atatcgaacc cggattttcc
    11041 ggaaaagtga actattccaa aggaaaactg gaacattttg atgcagtgat cgcaacagga
    11101 tcggataatt cttcgcgcta tttcgaacat tatttttcgt cctggcctca catcattcgc
    11161 aaaaacagaa cttcggttgc cgtgttgtcc ggactggaaa cagaggaaca actcaaatcg
    11221 ctgggtcacg atatttttga ttattacgga ctcggatgcc gcagtatctc caaggttttt
    11281 attccgcgcg atttcgatct ggaccgtttg tttggcgcga tctattcatt caaggatgtc
    11341 attcatcata aaaaatacgc caacaatttc gattaccaca aagcattgta tctgatgaac
    11401 agagatcagt tgctggagaa tggatttctt ttggtgaaag aagatgaagg acttcattca
    11461 ccgttgggta ccttgtttgt tgaacgatac gtttcgtacg atgaggtgga aaataaactt
    11521 gccggacttg aagaaaaaat tcaatgccgc gttggaaccg gaggaattcc ttttggtgat
    11581 gcgcaacgtc ctgcactttg ggattatgcg gatggagtag atacgatgaa atttttaata
    11641 ggattacaat agtggatacc ctgcgcaatt atatcaactc cattcgccgc gactttgcga
    11701 atcgtccgct ggatgaatct tctgttcctg ccgatcctta ttatttgttc gaacgttggt
    11761 ttgatgaagc tgtcggagct caggttcttg atccgtatgc aatgatcatt gccactgccg
    11821 gattggatgg tcagcctact tcgcgtgtgg tgtatatgcg tgatatcagt aagtccggac
    11881 tagtgttcta caccaatttc gaatcgaaca agggcaagaa tctcatcgaa aatccgaagg
    11941 catctgcatt gtttttttgg ggagatctcg atcgtcagat tcgtgtggaa ggtcgtgtgg
    12001 tgaaggctga tgatgaatta tccgatgctt atttttccaa acgtccgcgc gaaagtcaga
    12061 tcggtgcatg ggcaagtgcg cagagttctg ttttgctttc acgagcagaa cttgaacaga
    12121 aagtggagca gttaacccgc gagtttgaag gaaaagatat tcctcgtccg ccgttttggg
    12181 gaggattttt gctgatgcct gattcgtttg aattctggca aggacgacca agccgacttc
    12241 atgatcgcat actttttacg aaagcaggcg aagcctggca gacatcgcga ttgtcgccct
    12301 gaagcgaata ttcatttcgt tttttgcaat cccttttcct gcattcctca tgcaagggtt
    12361 ggtctgattt tgtccttcaa ttgaattctt cggtccggaa aaccttttcg tttaaacaga
    12421 aaaaaatgca aagcatgtac tcaatgcttg atgatctgtt gcagaaattg gaaggggtat
    12481 aaaaacaaaa atccccgccg gggcggggat tttatagatg tagtgttgat tcttagtttg
    12541 cagctggagc agcagcagcc ttcttacggg gagcagcttt tttcttagca gcagctttct
    12601 tcttaggagc agccttcttc ttagtagcgg ctttcttctt aggagcagct tttttcttag
    12661 tagctgtttt tttcttagca gcagcctttt tcttgggagc agcttttttc ttagcagcag
    12721 cctttttctt gggagcagct tttttcttag cagcagccnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12841 nnnnnnnnnn nnnnnnnntt cttcttagga gcagcttttt tcttagcagc agcctttttc
    12901 ttaggagcag ctttcttttt ggttgcagcc ttcttcttag gagcagcctt cttttttgtt
    12961 gcttttgcca tgatgtttaa tttttaaggg tttgattagt gatgtaaatt tatgttattg
    13021 atttcttata tcctatttat taggttattt ttttccacca cgtcttgtgt aaaactccga
    13081 ttcaaacaat tctactcttc gcaacacttt cttcatccac cttaatctga tttcattttt
    13141 ttcatcttca ttcaactgcc actcgatctc actcgcatgt aatcgctgca ccaattcata
    13201 cgcaatgatc gatgctgcaa cagaaatatt ataactctcc gtaaagccgt acatcggaat
    13261 ctgaatgcat ccatcggctt cttcaatcgc tgttgcactc aatccttctc cttcatttcc
    13321 taaaacaatc gcaacctttt catccaaagg taatgtgtcg ggcgtgtatc cgtttatgtt
    13381 tggtgtggtc gcatagatct tatatcctgc cgcgcgcaat cgctcgtaac atgtccgggt
    13441 gttgttcgga ttcttcttgt gcttgtgcat cctgatccac ttcgatgcac caaccaccac
    13501 atccttattc agttcataaa cattgttctg ctcgatgatg tggatgtcct gcaatcctaa
    13561 acattcgctg gttcgtacaa ctgcacttgc gttttgtggc tgatagatgt cttcaatcac
    13621 catcgtgatg tgacgcgtcc tgttgctgag tacttcctca aatcgctgaa gccggtcgct
    13681 ggtgatgaac tggctgagga attctatata aggtacgatc agttcctgat ccattgttca
    13741 aatataatca ttccacgcat tatcaatgca taacaaggat tgaaccttct tcctcatcgc
    13801 gtgtacaggt ttaaaattga tcacgtatga aacgtttttt gttcctcctc ctcttttccg
    13861 ttttgcagat gttgcaatcg gtcaacgcgc aaatggctat cacttccgaa tcctgggata
    13921 gccgaagaat gcgcgaaatg aatgtgcagc aaatggctgt tcagaattcg tacgaagttc
    13981 tggccatcgc tgttgatgct accatccgcg atcagatcgc tgaagtttct gtaacgcaaa
    14041 ccatttacaa cccgggcaac caggcgctcg aagtggagat cttcttccct ttacctaatg
    14101 gtggtatcgt gcagaatttt atgatgatgg tgaacgggaa agaaattccc ggacaacttc
    14161 tcaaacagga agaagcacgt ggtatctacg aatctatcgt gcgacgcaaa cgcgatcctg
    14221 ctttaatgga gtatgtgggc tacggtttat tcaagacttc cgtattcccg attcaggttg
    14281 gtgaagaacg gaacatcacc gtgcgctaca cacaggtttg cgatcgtaat ctgaatgcag
    14341 tcaacttcag ctatcccttc ggtacccaaa aattttctgc taaagcgctg cgctctcttt
    14401 cgttcaatgc ccgcatcgaa tcaagcgacg gaattaaaac ggtgttcagt ccaaccgacg
    14461 acattataat aaagcgcacc ggcgataaga cggctcatat taaaatggag aagacctatg
    14521 ttttgcctac ttccgatttt aaaatggtct tcaccacaga taaaggtgaa gtgggtgcat
    14581 ccttattaag ttataaagaa gcgtccgata aagatggcta cttcatgttg ctggcaagtc
    14641 cttctgttga tgaaatcgta aagcaatcgg aaaagacagt gctgttcgtt ctcgatagaa
    14701 gcggatcgat gtcgggacaa aaaatagaac aggcaaaaaa ggcggttgag tttgtaatgc
    14761 gcaatcttaa cgataacgac ctgtttaata tagtgtcgta cgacgaccgc atcgaaacct
    14821 ataaaccgga actggttcgc tacaacaaaa aagcctacga agaatgttat acctatatta
    14881 atggtatacg tcccggtggt ggaaccaata ttaatggagc attggtagca ggaatggaaa
    14941 tgctgcacga tgataaacgt ccgacttatg tgatcttcct taccgatgga ttaccaacag
    15001 caggagcaac agatgaactt acaattgcaa ataattgcaa gaacgcgaat aaagtaaaag
    15061 cacgactctt tgcttttgga gtaggtaatg atgtgaatgc acgtttgctt gatcgtttaa
    15121 gcagtgcgaa tggcggattg agtgaatacg taaaaccggc tgaggacatt gaagcaagtg
    15181 tggcgcgttt gtattccaat attagtagtc cggtactcac cgatatcaaa atcagttttg
    15241 aagcagtgga agtatttatg cccgtatcgg gattggacat ccgttccact tatccggaaa
    15301 ttatgcctga cctgtttcgc ggcggacaaa ttgtttgggt aggtaaatac aacaaaccct
    15361 gcaaagccaa tgtggtgatt cgcggaaaaa gtaatggtgt aaataaggag tatcgatttc
    15421 cggtagaatt tgtttcgcac acaggaacca aaacgcatga ttatgtagaa aagatctggg
    15481 catcgcgaag aattggtcac ctgatcaatc agatcgattt gaacggaaga aatacagaat
    15541 tggtagatga actggtacgc ttaagtaagg agtacggaat tcttacaccc tatactgcat
    15601 tccttgctcg cgaagatgtt gatctgaatg caatgagcca gaaccagcgt atcgccgacg
    15661 aagaattgaa agaactggaa gttgttcgcg gagaatcggc caacgatcag cgcgcgtaca
    15721 aatcgacgat gcaaaacaac accaaagtgc aaaactctgc gcctgttcag ggtggtgcat
    15781 ataaaaagga tgaacaggcc gaaaagaata tggcgaatgt tcgtaatgtt ggttcaaaga
    15841 ccttttataa taagaacaat caatgggtag atggaagtct ggatgccaaa accgcgaaga
    15901 atgtggtgaa ggtgagaatg tacagcgatg aatatttcaa actcgcaaaa ggacagtctg
    15961 ccgaattcaa tcagtacctg agtgtaggcg aaaatgttgt ggttgtcatt aataacgttg
    16021 cttacgaatt ctataaatga tgagttgcca ctaacaaaaa gggctgtctt tcgacagccc
    16081 tttttgtttg acgtatattt caattatgac cgaacaatgc ggtagcgtgc tataccggtt
    16141 ggtgtatgaa cttccagaat ataaatgcct ctctccaggt tttcgatgga gatggatgtg
    16201 gcggaggatc ggatattcat ccggtgaatc cgttttccgc tgaggtcgta aatcattaca
    16261 ttctgaataa atgccgaagg atcgctgcca acaattgata caacaccatc ggtaggattc
    16321 gggaatacgc ggaaggacga ttgaagaagc tcatcaactg cagccggcgg tgcaatggtt
    16381 ccgcggttgc ttcgtgtgct gttgtggtta atggcgcgtg tagcttcgca acttgtaggt
    16441 ggacgaacat ctacagtgta tcggagcaat ccggatgccg gcgcagttac atccacatag
    16501 gcatgaagcg tagaggggac actgtcgatg agttcccatc ctgtacttgc atggtaacgg
    16561 ttgatgtaaa cagttggata gttgaatcct tcgtaggtat cccaaatcag attcacttcg
    16621 ccgcttaatc cgcgactgct taccagatga agtgttttgt gattggttcc gaacgacgat
    16681 tcgtttccac aggtatcaac catagtcact ttgtatctcc atccgcgaat aacaggattg
    16741 gccgattgat cggtccactg actgagtgaa tcgaacggtt tggtgaacca gaaatcgtac
    16801 tgaccggcca ctgcattttc gcggtagatg ttgaagcgat cgatgttgaa tagattgggt
    16861 ttttcccaaa cgcataatac ctttccgctg gcagaatcta ctgtcgacat acacagcgta
    16921 atcggatcgg gaaaaattcc gggaacggta gcagaagaat gcgacatgca tccgtttcca
    16981 ccggttactg aaagatgata ggtgccttga agtaagttgg aaatatcctg agttgttgcg
    17041 ccgttgctcc aagaataggt gaatggtgga gtacctgaag tcacattcat attaatagca
    17101 cccaacatcc ctgaacaatt cgccggcatt acacttacga gttgtgttac cggagcattc
    17161 ggattgctga gtgaaatgtt ttgtgtaacg gagcagttgt tgttatcgct gatggtaact
    17221 gaataaattc ctgaaggcaa tccgctcacc gaagatgttg ttgcgcttgc tgcattggcc
    17281 gaccacaaat atgtaaacgg agaagcaccg gttggattca ttgcgattga accatcagat
    17341 gccccacaag caggttgaga aattacggga gtccctactt gaatagaact aacaccaaca
    17401 ataataccag ctccggttat gcatcctgct gcgtcggtaa tttgtaccat atacgaaccg
    17461 ggtttaattc ctgcaagatc ttcggtggtg gaagagttac tccatacaaa agagaatgga
    17521 ggagttccgc cggttacagt aagatcgatg gcaccgttgt ttgaacctgc acaggtggca
    17581 ttggttacaa ccgacgaaac agttggtgcc ccggaactcg ataacataaa tgtatgggca
    17641 gcggaacaac cattggcgtc gtaagcggtt atcatataag taccggctgt cattgaatcg
    17701 gccacagata tattatcacc gttcgaccaa ttgtaggtgt aaggcatcac tccgtttgaa
    17761 accgcaacac tagcagaacc gttatccatt ccgcatgttg gtgatgtagt gcttacgttt
    17821 aaggacatag aagttggatg aggaacattt acagttaaca tggcatcaca acctgttgca
    17881 tcatgaatta caaccgtata atttcctgca gtaattgtag aaagatcctg tgtggttgat
    17941 ccgttgctcc acatataggt ataaggagat gttcctccac ttaaactgag atcaattgat
    18001 ccgttgctcc cttgaaaaca ggagggcgaa acggtgcttg ccgatgcagt gatctgacaa
    18061 ggtgaacctc ctgcaggtaa attgtaaagt gaatcaacct gagaattatt tagcacacaa
    18121 ccgtaaacgc gaacatcatc cagttttcct ttgtagaaat gtgtaaaatc aacgattgca
    18181 ccaaaaatga aattggtggt catttgggtt ggccaacttt tgggttcctg gttgatgtat
    18241 tgaccattga tgtataattt tgcaacggaa ccatcgtaag tagcaacgac atggtgccat
    18301 tggttgtaga cgccattgaa tccaacaatt acattatcat ttcctcctga atatgtcaat
    18361 gtattcataa ctcctaatgc gttggttgtt ggaagtattt cctgcgagga gcccgttcct
    18421 gttcctccgt aatgaaacaa acgatgataa ccattgtaga tcgtatcgct cacattaaac
    18481 cataattcga tggaacgctg gttgtatccc gcaggccagt accaggttgg acctgtaatg
    18541 aacgaggagc ttccattgaa ttgcactgcg gattgtggta caccaaatcg atcgtttacg
    18601 taagtaatat cagttgcagt accatgctgc gaaaaaccac caccatcgtt tacgtttcct
    18661 gaaaatttgt attcggcctt tagacatccg cctctttgat atagggcgtc aatctgactt
    18721 tgattgagtt caaacgccca gattcccaat tcatcaaaca taccaataca aggtaatcct
    18781 ccgtatccca tagtgaaatc attccagttg tttaatgttt gaggaaggct atagatattg
    18841 ctgccattga ggaaaacttt caaaatacct gatccaaaag ttaccacaat atggttccac
    18901 tgattttgcg ggaagttggg taatgtcgca ttaatatttg gagtggcttg ttgaatctgg
    18961 agttgacctg atgaggttgt tcttaattca agaccaccgg accagttcaa caggatgttt
    19021 tgctgagagg aattatgcct gaaccaaaaa gaatagcttc ttggtccact ggtaatgttg
    19081 atacctgtta catccatttt tccggttccg gtaaaatcaa cggctttgtg gttgttccca
    19141 aatcggtcct gcatgccagc tacagttccg gaaaaaactc cattcagcgt gggcgtaact
    19201 tcacaaaccg gactgttgtc gagattgtac catgccacca atcctgcttg cggaacgttt
    19261 gatggaattt gagcgaaggc gatagatgta ctaaaaagac tgcatataag tatgcatacg
    19321 tgaatagaat aaagacgcat agtagtcaaa tt
//
LOCUS       DAOMYG010000007        18287 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1048_length_18287_cov_8.556494, whole genome shotgun sequence.
ACCESSION   DAOMYG010000007 DAOMYG010000000
VERSION     DAOMYG010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 18287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18287
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1048_length_18287_cov_8.556494"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1021)
                     /locus_tag="PK610_00535"
     CDS             complement(35..1021)
                     /locus_tag="PK610_00535"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ34438.1"
                     /translation="MKFFLTGGTGYIGSWMIRRLTERGHEVHALVRNPEKAKSIQNDL
                     VKLFRGDMLDEEAIRRGMEGCDGVFHLAAFAKVWARDPKVFFDQNVVATNRILDAARD
                     LGVKRTVVTSTAGVFGASLHGTITEESARDLDFFNEYESSKALSESKIKDYVIRGLDV
                     VIVSPTRVYGPALHGSPESVTLLIDKFVNGSWRFIPGDGTKIGNYVFVEDVVEGHILA
                     MEKGKKGETYILGGSNHDYNEFFGKLSELSGVKRSMIKMPIGLQMMFSRFQLLKTKFG
                     GEPLITPKWIAKGKYDWKVDASKAVRELGMIITPLETGLKRTIDWSRERRKQ"
     gene            1312..2547
                     /locus_tag="PK610_00540"
     CDS             1312..2547
                     /locus_tag="PK610_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HRJ34439.1"
                     /translation="MAKMELMSPAGSREALMGAILAGCDSVYFGVEQLNMRARSSNNF
                     TLDDLRDIASLGKEHKVKTYITLNTVLYDHDMQLMRSIVDAAKENGISALIAADHAVI
                     NYARKIGMPVHISTQASISNIETVEFYSAFADVMVLARELSLMQVKEITREIQRRNIT
                     GPSGELVRIEIFAHGALCMAISGKCHLSLHSYFASANRGACMQNCRKSYVVTDKEDGT
                     EFEVDNEFIMSAKDLCTIGFLDQIAGAGVSVIKIEGRGRAVDYVHIVTQCYREALSAI
                     EEGSYNQEKINSWMDRLATVYNRGFWDGYYLGKKMGEWNNENGSKSTKTKVYLAKGQK
                     FFREANVGEFIMEAHSLNTGDEIIITGPSTGYLHSIVEEIRLDDQSVESVKRGEVFTL
                     RVPDRIRPSDRLYKLVSES"
     gene            2544..2777
                     /locus_tag="PK610_00545"
     CDS             2544..2777
                     /locus_tag="PK610_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRJ34440.1"
                     /translation="MIRIIQHREKCIGCNACVEAALGRWRVSRKDGRCTLVGGVDKKG
                     FFSVVVPDHEEEENQIAARNCPVRIIRIEKIRK"
     gene            complement(2962..3705)
                     /locus_tag="PK610_00550"
     CDS             complement(2962..3705)
                     /locus_tag="PK610_00550"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HRJ34441.1"
                     /translation="MTYDFTWKESDIITFVTIVGSTFFFSVYWFIFASEKIKANFYKK
                     YPNNDEGTIRHVLFTKYAGFILMGLAPAVLFLILFPQYTLSDYGIAFGKGTDLLTLYW
                     CLGLGIPIIIMNYFAAQRPKTFSMYPQIRAKEWDRSLIIRYSLGWCLYLFGYEFLFRG
                     ILFIPLIDTLGVWPAIAVNVALYAATHIPKSLDETIGAAPLGFILCLITLQTGTIWVA
                     FVVHVFLALSNSLFALKFHPEFKIAKKRS"
     gene            complement(3714..4619)
                     /locus_tag="PK610_00555"
     CDS             complement(3714..4619)
                     /locus_tag="PK610_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34442.1"
                     /translation="MKKLLLLALLVLPCSGLFAQQDTSMIGKMIVVELYSGGEQKGTL
                     MADDGREITIMTTDRGKIIIPKYQVKSMRLINGQRKKDEDYIMQNEYGNHYQFFSNAV
                     PYNENEVTLRMPLFAAFIVDIGLTEEFSVSFGSIWFSAYGLTGTYRKALGNDHHLSFS
                     LGAGGMLARFGRNSSYNDLSGMLFKGCYSIGTKEKNFSFGLGYGSTFSGNVNSIVFHT
                     GGLVQLNQKIAMTVEGMMAPKYSAGQGGWALRIRTKKDNCWDLGVHFVLYEEEYVAYS
                     PTGQTYLEKGISGFGFPFVGYMHKF"
     gene            complement(4629..5621)
                     /locus_tag="PK610_00560"
     CDS             complement(4629..5621)
                     /locus_tag="PK610_00560"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ34443.1"
                     /translation="MDKSLFITGADGLLGSNSVRELLAQGYSVRALIQPGRKVKSLEG
                     LPIEKVEGDLLDETKLRNAMKGCRYVIHGAASTAMWPDNNPVVRKVNIEGTRCLLNAC
                     KENGVERLVYIGTANSFGAGSIDQPGNESKPYTAAKYKNDYMASKYEAHQLVLDYVKK
                     GLNAVIVNPTFMLGAYDSAPSSGAMILAVVNQQVPGYSPGGKNYICVKDAAKGVVNAI
                     EKGRTGECYILGNENLNFKDAFAKMANVAGVKAPGFAIPKWVLLLYGGMGSTVARITG
                     KAPKVSYTIARISCDEFYYSSLKAVKELDLPQTPIENGIREALDWFKENGYLKN"
     gene            5858..6880
                     /locus_tag="PK610_00565"
     CDS             5858..6880
                     /locus_tag="PK610_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34444.1"
                     /translation="MLYHFAFYTLLFQALIFPLLLIWTIYFVVVNLLKPSQPKQKAED
                     KKSKNEKEQFNGPHIDACECHNCRAPLPLSSDLKECKQCGTPFSMPAELLEIVSRREV
                     ANKRLNEAIRYWKKARRMTSQWLRSGILLLIVWLVATPVIILIESSYRSDFDALIEKW
                     GSIGSALVGISFFTLIFWVLILFITRGIISPKRRALLPELDFKKSDALHDSDMPCEHC
                     NAQLSIEKGNFSVVCNYCGTENFRKSFAWKMLNETKKHQHAVSSSLLQAMDDYRSAIE
                     DALHTPAILIFILIILPFFMVVVPMYLFDLIAEHLFITTSIVVGGIAAWFIRDTYFPA
                     KKKINP"
     gene            complement(6870..7928)
                     /locus_tag="PK610_00570"
     CDS             complement(6870..7928)
                     /locus_tag="PK610_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRJ34445.1"
                     /translation="MSYYISGIQQMGIGVPDVQAVFRFYRKAFGADVRIFEEAAQAPL
                     MINYTGGVVQSRTATMAINMNGGGGFEIWQFTSRSTEKARFDIRIGDTGLFVTRIKCR
                     DVKAQYALHQEAGLDIIGALSTTPSGESHYFLRDPNGNIFEITEGKSWFSNAPHLCGG
                     VEGSMIGVRDIEKAKKLYSDLLGYNKVEYDVNGKFEDLAHLPGGEHELRRVLLSHSKE
                     RQGPFSKLLGATRIELVQVTSRPAQTIFENRMWGDWGFIHLCFDVRNMDELKTAADKA
                     GFPFTVDSGSSFDMGEAAGRFAYVEDPDGTLIEFVETHKLPILKKFGWYLNLKKRSPV
                     KALPNWMLRTLRFSRVKD"
     gene            8103..8831
                     /locus_tag="PK610_00575"
     CDS             8103..8831
                     /locus_tag="PK610_00575"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRJ34446.1"
                     /translation="MKRLIFVFAMAALVACGSKNPMEESVNVEKKEDKLSYAMGVYIG
                     QDIAKMLKMQGQDSIIDKSLLVRGLYDAILEKETAIPAESAKQVVSDFLREQDEDRRK
                     AQMAQFEGIRQEGENFLAANKEKAGVTTTLSGLQYEVMKAGKGPNPKLGDSVEVHYVG
                     TLLNGTKFDSSVDRGETFKFEVAYGGLIEGWIEGVQLMNEGAKFRFWLPYGLAYGDRG
                     AGQDIPPFSALVFEIELIKLTPKK"
     gene            complement(9029..9535)
                     /gene="msrB"
                     /locus_tag="PK610_00580"
     CDS             complement(9029..9535)
                     /gene="msrB"
                     /locus_tag="PK610_00580"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRJ34447.1"
                     /translation="MQTLLPIFIFFSSWMLQSSCTSPVNEQANLSIPQTDTTLKKLVL
                     TDTQWRERLSAEAYYVLRKKGTERSFTSSFHNSKRKGNYHCAGCDQLLFRSSEKYDSG
                     TGWPSFFQPAFSNSVSEAEDRSYGSIETEVLCAKCDGHLGHVFKDGPRPTGLRYCING
                     VALVFKEN"
     gene            9558..10142
                     /locus_tag="PK610_00585"
     CDS             9558..10142
                     /locus_tag="PK610_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34448.1"
                     /translation="MNRSSALTIFNLASDSVDSEEIRDAYEQAVFEISNRFLRNHPQP
                     FLVRAKIKRLQTFSEAWLVLTGQSPGPLPDLPERKLDLGGLEPLLRSYEAHLAALRTE
                     LAKCREALAAAAVMERMAELQEQWDKELVRRFPLEEDQWKAVSVQQMPDTVAMMKVIS
                     EKGEEQVMAGESVHLQQPFISESARIHRQNSRAN"
     gene            10211..10414
                     /locus_tag="PK610_00590"
     CDS             10211..10414
                     /locus_tag="PK610_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34449.1"
                     /translation="MFKFIVPNNNQAHAMAMALFDESAQVESRVSGKGSYISLTAREM
                     MVSVDSILEKYRAAYAIDGLIAL"
     gene            10416..10802
                     /locus_tag="PK610_00595"
     CDS             10416..10802
                     /locus_tag="PK610_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34450.1"
                     /translation="MTSAFFIVLLYSLTVTVGVFLMFFGYRYIINRMSGKHSGRKYVK
                     VRRIHEGPFSGESFLVFDLPEQAAIRFAIQDNTEQEIAVLLEEECRMGELVIHVDTTK
                     FPNGQYYFELIAPGQRIQRKFSISNV"
     gene            10799..11515
                     /locus_tag="PK610_00600"
     CDS             10799..11515
                     /locus_tag="PK610_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34451.1"
                     /translation="MKKALIGFLLFAFPFVLTAQEEGETSGNVRPEKIPDIRYGFKGS
                     FKLPTPIKNKAFTSVIDGIADANLSFHYPFLRHFYFGAGYRYSYFQINDFRAKADLNA
                     NLQIHSPFAVLGFERFATPRFLYGVSLRSGYSMLHFKSNTCLSNNAEGKGAGQEAVFI
                     EPEFTLELMSGENMAFSFVTSWTWILDEFGAENICLSNFSGLTADDSKGYYQFISVGF
                     GFTVFLGGEKRSKGPRIYYD"
     gene            11603..13693
                     /gene="ppk1"
                     /locus_tag="PK610_00605"
     CDS             11603..13693
                     /gene="ppk1"
                     /locus_tag="PK610_00605"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRJ34452.1"
                     /translation="MAGKKEKKVKKSEKFEVINRDLSWLSFNARVLQEADDDRVPLVE
                     RMRFLGIFSNNLDEFFRVRVATLRRMGVLDTNAKKEFGYDPKKILDRIQKTVVEQQKE
                     FERIFQEIIRKLESKGIVLLNEKQLSKEQGAFVRNYFQTTVRQTLVPIMLDKKSKMLN
                     LKDKMIYLAIKLSRSTDRSDFRYALIEVPTQVVSRFLVLPQGKSNKTNILLLDDVVRF
                     CLDDVFQIFDYDVLEAYTIKLTRDAELDIDDDMQQGMVEKIQRSLKKRKLGQLVRFIY
                     DAEIPPDLLIFITRKLKLENNKNLIAGGRYHNFKDFIKFPGIGDASLQNEELPPLTHP
                     DLINQRSILDVIRKKDVLLTFPYQSFNYIIELLREAAIDPKVEHIQINLYRVGEKSRV
                     VNALLNAAKNGKQVTAIVELQARFDEENNIKLSRKLQDEGVKVIFGVPGLKVHSKLLL
                     IHRKERKKEVLYAHVGTGNFHEVNSRIYTDHALLTADERITTEVEKVFEFFLRNYERA
                     NFKSLFVSPFNTRKRFIELIQQEIHNAKRHHPAYITLKMNNLVDEEMIRWLYRASQAG
                     VKIKIIVRGICSLIPGVSGMSENIEAVSIVDRFLEHGRVFIFCNDGKELVYISSADWM
                     QRNLDHRIEVSTPILDTQVKKQVLDMINIQLKGTAKSRIIDREQTNRYKGEGKKDLLR
                     SQIEIYNYLKKLKG"
     gene            14100..14981
                     /locus_tag="PK610_00610"
     CDS             14100..14981
                     /locus_tag="PK610_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HRJ34453.1"
                     /translation="MIYGAIDIGSNAGRLLIGNVVEEDGMASIKKFSLTRVPLRLGED
                     VFTDKKISPARVKMLIKTLKAYKHLMDVYDIVDYRACATSAMREAENRKEILEEIKKE
                     TGIKLEIIAGEEEAELIFSTINTMKFERNKSYLYIDVGGGSTEVSLLENGERKKSKSF
                     KIGTLRILKGKVDEKRWLELKDWVKTLNPEHKQILAIGTGGNINRMLKINRSEDDNSI
                     SYSDIKRIQTHIASYSLNDRINKLGLRPDRADVIVPAADIYLAVMRYADVTQMLVPKV
                     GLSDGIIYELYKKNRKK"
     gene            15105..15434
                     /locus_tag="PK610_00615"
     CDS             15105..15434
                     /locus_tag="PK610_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34454.1"
                     /translation="MQHPFRQEKSIFDGQFLLITEDNEFRMSLWVKGFGIHSANGEEV
                     LPLESSFNLIRYEESGNSLIVHFDVYPNGRKQYDAEINLFNRTFTYNKQTMGLDKFVG
                     TFMSIQD"
     gene            15507..16268
                     /gene="map"
                     /locus_tag="PK610_00620"
     CDS             15507..16268
                     /gene="map"
                     /locus_tag="PK610_00620"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRJ34455.1"
                     /translation="MSISNEDELLGVQKVSDVVALTLREMKAFARPGMSTKQLDDFGA
                     ALLNEFGAHSAPLKTYDFPGCTCISVNSEFCHGIPSIHRILQEGDLINIDVSAELDGY
                     WSDNGASFVLGDDFHGHQQLVDASKEILQRAINSIRGGLKIADLGHLIETEAKKRGYK
                     VIRNLTGHGIGRSLHESPAEIANFRDKYNHNRFKKNAVVAIETFISTHSDIAVTLKDG
                     WTMVGNKGGFMAQHEHTIVVTDVEPIILTWNNGIW"
     gene            16779..>18287
                     /locus_tag="PK610_00625"
     CDS             16779..>18287
                     /locus_tag="PK610_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRJ34456.1"
                     /translation="MSLILTAGLLLGFRGGLFIGLLTISTDFYFVYAEINGFLPEPTV
                     MHTPMTRWISNIIPFGTILALQYYSTNHLRSSLVATQREVALREEAERVLKQTVDNLK
                     ERVKEQKTLYRLSQVLRNEEVGIPELTKQIVETLPAGWQFPDYLKARVRIDEIEFSSS
                     GFEESEQRQYVKLNTSSGTTVVIEVVYASGVPCKDPQLFLKEEEHLIKMCAEMIKISM
                     DSRENKKELEEYKYALDIGSIVSIADVNGIMTFVNDNFCKISKYEEHELIGKHYGIIS
                     SENHSDDFFKGLANEMKKGKPYRGEFCNRAKDGSVYWVNTSIVPFLDQNGKIYQYLSI
                     NHDITDRKNADEKIKESEKLLKKITSQVPGNTYMFEIEESGKANLLFVSRGTDLFNHQ
                     YSLEELNEHPERLQEVLFEEDKEKFNDAMIDAHKTRLPINFQYRIVINGHIRWRWMQA
                     IPEVDTKGRVLWYGATSDITPLVDYITSIEQIIFDISHVLRRPVASMKGMSKL"
BASE COUNT         5215 a         3792 c         4106 g         5174 t
ORIGIN      
        1 attctttttt ccggatgatt ttcatagcgc cggattattg tttgcgtcgt tctctcgacc
       61 aatcgattgt tcttttcaat ccggtttcaa gtggagtaat aatcattccc aattcacgaa
      121 cagctttgga tgcatccacc ttccaatcgt attttccttt ggcaatccat ttgggagtga
      181 tcaaaggctc ccctccgaat tttgttttta gtaattgaaa acgcgaaaac atcatttgca
      241 atccgatcgg cattttgatc atgcttcgtt ttacaccgga taattcactg agctttccga
      301 aaaactcgtt gtaatcgtga ttactccctc ccaaaatgta tgtctcgcct tttttgcctt
      361 tttccattgc gagaatatgt ccttccacca catcttccac aaagacatag ttcccgattt
      421 ttgttccgtc acccggaatg aatctccagc tgccgtttac aaatttatca atgagcagcg
      481 ttacactttc cggcgaacca tgcaatgccg gaccgtatac acgcgttgga gaaacgatga
      541 ccacatccaa accacgaatc acatagtcct ttatttttga ttcactcagt gctttggaac
      601 tttcgtattc gttaaaaaaa tccaggtcgc gtgccgattc ttccgtaatg gttccatgca
      661 gtgaagcgcc aaatactcct gctgtggagg taaccactgt gcgcttaaca cccaagtcgc
      721 gggcggcatc taaaatgcga ttggttgcta ccacattctg atcgaaaaac actttcggat
      781 cacgcgccca aacttttgca aatgcagcaa gatgaaaaac accgtcacaa ccctccatcc
      841 cgcggcgaat ggcttcctca tcgagcatat ccccgcgaaa aagttttacc agatcgttct
      901 gaatggactt tgctttttca ggattccgca ccaaagcatg gacctcatgt ccacgctccg
      961 tcaatcgcct gatcatccat gagccaatgt atcccgttcc cccggtgaga aaaaatttca
     1021 taaggcaaag atagggaaga gaaattgttt tttggtgaat agtgatcggt gaatggtgaa
     1081 cagagatccc gacgctatcg gtcgggacag gtcggttaac agtgatcggc tcgtcttccg
     1141 tcttcgttcc cgtcctcgtc ttccgacttc gttcccgcct gacccgaccg accggtcgga
     1201 tagcatcggg attccttttc cttttggttg gaaatcggta atcaatcccc tgacgataat
     1261 catattttca ggtattccgg ttctgtaatt ttgcctgtta aatttcaaca gatggctaaa
     1321 atggaactga tgagtcctgc gggttcgcgg gaggcgctga tgggtgcgat actggcgggt
     1381 tgcgattcgg tgtattttgg agtagaacaa ctcaatatga gggcacggtc gagcaataat
     1441 tttacgctgg atgatctccg tgatattgct tcattgggta aagagcataa agtcaaaact
     1501 tacattacgc tcaatacggt gctctacgat cacgatatgc aattgatgcg atcgatagtg
     1561 gatgctgcaa aagaaaacgg aataagtgca ctcatagctg cggatcatgc tgtgatcaat
     1621 tacgcacgta aaattggaat gccggtacac atttccacac aggcaagtat ttcgaatatt
     1681 gaaacagtgg agttctattc tgcctttgca gatgttatgg ttctggcccg tgaattgagt
     1741 ctgatgcagg tgaaggaaat cacccgcgaa attcaacgaa gaaatattac aggaccgtcc
     1801 ggtgaactcg tccgcattga aatttttgcg catggtgcac tttgtatggc gatctcagga
     1861 aaatgccatt taagtctgca cagttatttc gcttctgcca atcgtggtgc ctgcatgcaa
     1921 aactgcagaa agagttatgt ggtgaccgac aaagaagacg gtaccgaatt tgaagtcgac
     1981 aatgaattca ttatgtcggc gaaagatctt tgcacgatcg gattcctcga tcagattgcg
     2041 ggtgccggtg taagcgtaat aaaaattgag gggcgtggtc gtgcagtgga ttatgtgcat
     2101 atcgttacac aatgttaccg cgaagcactt tctgcgattg aggagggtag ttacaatcag
     2161 gagaagataa atagctggat ggatcgtctg gcaacggttt acaatcgcgg tttctgggat
     2221 ggttattatc tcggtaaaaa aatgggtgag tggaacaatg agaacggatc caaatcgacc
     2281 aagacgaaag tatatcttgc caaaggacaa aaattcttcc gcgaagcaaa tgtcggtgag
     2341 tttataatgg aagcacatag cctgaataca ggggatgaga ttatcattac cggacccagt
     2401 acaggatacc ttcatagtat tgttgaagag atccgacttg atgaccaatc ggtggaaagc
     2461 gtaaaacgtg gcgaagtctt tacgctgcgt gtacctgatc ggattcgtcc ttctgatcgt
     2521 ctttacaaac ttgtttccga atcatgatcc ggatcattca gcatcgtgag aaatgcatag
     2581 gatgcaatgc ctgtgtagaa gcagctctgg gtcgctggag agtttcgcgt aaggacggac
     2641 gctgcacatt ggttggagga gtggataaaa aaggtttttt ttcggtagtc gttcccgatc
     2701 atgaagaaga agagaatcag atcgcagcac gaaattgtcc ggtgaggata attcgaattg
     2761 aaaaaattag aaaatagtga atagtgaata gtgaacagtg aaaaatggtc cagtactctt
     2821 aaccgatcac ttacaactac tctgtgaata gtgaaaggtt caactactct taacttttaa
     2881 ctaattactc ataacaaata tctgaacatt agaatgaaat tggagaaatg gttccaataa
     2941 atatggtgat gcaaaaaata actaactcct cttttttgcg attttaaatt cggggtggaa
     3001 ttttaatgcg aaaagtgaat tgctcagtgc taaaaatacg tgaaccacaa aagcaaccca
     3061 aatggttccg gtttgcaaag tgatcagaca caatataaat ccaagcgggg cggcaccgat
     3121 cgtttcgtca agacttttcg gaatgtgtgt ggctgcgtat aaggcaacat tcacggctat
     3181 tgcaggccaa actcctaatg tgtcgataag tggaataaat agtattccac ggaacaaaaa
     3241 ttcgtagccg aataaataca aacaccaacc cagcgaataa cgaataatca atgaacgatc
     3301 ccattccttt gcacggattt gcgggtacat ggaaaacgtt tttggtcgtt gcgctgcaaa
     3361 gtaattcatg atgatgatag gaatacccaa tcccaaacac caatacaatg tcagtaaatc
     3421 cgtgcctttt ccgaatgcaa taccgtaatc cgataatgtg tactgtggaa agagaatcaa
     3481 aaacaatact gccggtgcaa gtcccattag tataaatcct gcgtacttgg taaacaaaac
     3541 atggcgaatg gtgccttcgt cgttgtttgg atatttttta taaaagttgg ctttaatttt
     3601 ttcagatgca aaaatgaacc agtaaacact aaagaaaaag gtggagccaa caatggtaac
     3661 gaatgtaatg atgtcgcttt ccttccaggt gaaatcgtaa gtcatggtgt aatttaaaat
     3721 ttatgcatat agccaacaaa aggaaatcca aatccggaaa tgcctttttc cagataggtt
     3781 tgaccggttg gtgaataggc gacatattct tcttcgtaca acacaaaatg aacaccaaga
     3841 tcccaacagt tgtctttttt ggtgcgaatc ctcaatgccc aaccgccttg tccggcgctg
     3901 tatttgggtg ccatcatacc ttcaacagtc attgcaatct tttggttcag ttgtacaagt
     3961 ccaccggtat gaaacacaat tgaatttaca ttgccggaga atgtacttcc gtagccgagt
     4021 ccaaatgaaa aattcttttc ttttgttcca atggaataac atcctttaaa caacattccg
     4081 gaaagatcat tgtaactcga atttctgcca aagcgtgcca acattcctcc tgcgcccagc
     4141 gaaaaactta ggtggtgatc attccccaat gccttgcgat aggttcctgt taatccgtat
     4201 gcagaaaacc agatcgaacc aaaggacact gagaattcct ctgttaaacc aatatcaaca
     4261 ataaatgctg caaagagggg catgcgcagt gtgacttcat tttcattgta gggaaccgca
     4321 ttgctgaaga attggtaatg attgccgtat tcgttttgca tgatgtaatc ttcatccttt
     4381 tttctctgac cattgatcag tcgcatggat ttaacctggt atttcggaat gatgatcttc
     4441 cctctgtcag tggtcatgat ggtaatctct cttccatcat ctgccattaa tgttcctttc
     4501 tgttctccgc ccgagtacaa ttccaccaca atcattttac caatcatgct agtatcctgc
     4561 tgtgcaaaga gtccggaaca gggcaatact aaaagtgcga ggagcaatag ctttttcatg
     4621 gtggacaatt agttttttaa atacccgttt tctttgaacc aatcaagggc ttccctgatt
     4681 ccgttttcga tgggtgtttg cggtaagtca agttctttca ctgcctttaa agaactgtaa
     4741 taaaattcat cgcaggagat acgggcaatg gtataactta ctttcggtgc tttaccggta
     4801 atccgggcta ctgtactacc cattccgccg tatagcaata agacccattt gggaattgca
     4861 aacccgggcg ctttcactcc tgcaacgttt gccatttttg cgaaggcatc cttgaaattt
     4921 aaattctcat tcccgagaat gtaacactct ccggttcttc ctttttctat agcgttcacc
     4981 actccctttg ctgcatcttt aacacagata taatttttcc caccgggcga atagccgggc
     5041 acttgttggt tcacaactgc aagaatcatt gctcctgaac tgggggcact gtcgtacgct
     5101 ccaagcataa aagtgggatt cacaatcact gcattcaatc ctttttttac atagtccaat
     5161 accaattgat gggcttcgta tttagaagcc atgtaatcgt ttttgtattt ggctgcggtg
     5221 taaggtttgc tttcattacc aggttgatcg atgcttcctg cgccaaaact gttggctgtt
     5281 ccaatgtaca ccaatcgctc aacaccgttt tctttgcagg cattcaaaag gcagcgggtg
     5341 ccttctatat tcactttacg aacaacagga ttgttatcgg gccacatggc tgtgctggct
     5401 gcaccgtgta ttacgtagcg acaacctttc atggcattgc ggagttttgt ttcgtcaagc
     5461 agatctcctt ccactttttc gatggggagt ccttcgagcg atttcacttt tcttcccggc
     5521 tgaatgagtg cccgtacgct atatccctgt gcaagtaatt cacgaacgct attgctgccc
     5581 aacaagccat ctgccccggt aatgaaaagc gatttatcca tggggtaaaa gtactattaa
     5641 tgtggataaa gtagtcttcc ggtaactaat tccaggtaag aaaatcattt agtctcattt
     5701 ccagttcttc tgaaaaggag cggaacaaat acttcaagcg actgcgcgac cgggtggagt
     5761 gggtgagagg cagagtctga ttcttttcgc tattcaaatc cgatatttgt gtttccgaac
     5821 cgaagattta aatacattgg ggaataattc ccggattatg ctgtaccatt tcgcatttta
     5881 taccttactc tttcaggctc ttatttttcc attgttgctg atctggacca tttacttcgt
     5941 cgtcgtaaac cttctcaaac cttctcaacc aaaacaaaag gcagaggata agaaaagtaa
     6001 aaacgaaaag gagcaattca atggtccgca tatcgatgca tgtgagtgcc acaattgcag
     6061 agctccttta ccgttaagtt ccgatttgaa agaatgcaaa caatgcggaa cacccttttc
     6121 gatgccggca gaattactcg aaattgtaag cagacgagag gtggccaata agcgtctgaa
     6181 tgaagctatc cgctattgga aaaaagcacg acgaatgaca tcgcagtggt tgcgctcggg
     6241 tatattgctt ctgattgtct ggctggtggc aactcctgta attattctta tcgagtcatc
     6301 ctaccgatct gattttgatg cattgattga aaagtggggt agtattggga gcgctcttgt
     6361 gggaatctcc ttttttacgc tcattttctg ggtgctgatt ttgtttatca ctagaggaat
     6421 aatatcgccc aaacgacgag ccttacttcc tgaactggat tttaaaaaat cggatgcgct
     6481 gcacgatagc gatatgccct gcgagcattg caatgcgcaa ttgtcgattg aaaaaggaaa
     6541 cttttctgtg gtttgcaatt attgcgggac cgaaaatttc aggaaatcat tcgcatggaa
     6601 aatgctgaac gaaacaaaga aacatcagca tgctgtttcg tcctcactgt tgcaggcaat
     6661 ggacgattac cgaagtgcta tcgaagatgc tttacacact ccggctattt tgatttttat
     6721 cctgatcatt cttcctttct tcatggttgt tgttcccatg tatcttttcg atcttattgc
     6781 tgaacatctc ttcattacca cttccattgt tgttggcgga attgcggcct ggtttattcg
     6841 cgacacttat tttccggcga agaaaaagat taatccttaa ccctgctgaa gcgtaatgtg
     6901 cgcaacatcc agttgggaag tgcttttacg ggactccgtt ttttcagatt gaggtaccac
     6961 ccgaattttt tcaggatggg gagtttatga gtttccacaa attcaataag cgtaccgtcg
     7021 gggtcttcta cataggcaaa tcgtcctgcc gcttctccca tatcgaaaga actaccgcta
     7081 tcaacggtaa agggaaatcc tgctttatct gctgctgttt tgagttcatc catattgcga
     7141 acatcaaaac aaaggtggat aaatccccag tcgccccaca tgcggttttc gaaaatggtt
     7201 tgggcaggac gcgatgttac ttgcacaagt tcaattcgtg ttgctccaag taatttgctg
     7261 aacggaccct gacgctcttt ggaatgtgag agcaataccc tgcgcaattc atgttctcct
     7321 cccggtaaat gagcaaggtc ttcgaatttt ccgtttacat cgtattccac cttgttgtaa
     7381 cccagaagat cactgtatag tttttttgct ttttcaatat cgcgcactcc aatcatcgat
     7441 ccttccacgc caccacagag gtgtggtgca ttgctgaacc atgattttcc ttcggtgatt
     7501 tcaaaaatat ttccgttggg atccctcaaa aaataatgcg attcgccaga aggagtggta
     7561 gaaagtgcgc caatgatgtc gagtcctgcc tcttgatgca atgcgtattg tgcttttaca
     7621 tcgcggcatt tgatgcgggt aacaaacaaa ccggtgtcgc ctatgcgaat atcgaacctg
     7681 gctttttcgg tagagcggct ggtgaattgc cagatctcaa acccgccacc tccgttcatg
     7741 ttgatggcca tggttgcggt gcgcgactgt acaactcctc cggtgtagtt gatcatcagt
     7801 ggtgcctgtg ccgcctcttc aaaaatgcgg acatcggctc cgaatgcttt tctgtaaaaa
     7861 cgaaataccg cctgtacgtc agggaccccg attcccattt gctgaatgcc agaaatataa
     7921 tagctcatag tgctgtgttg tgccaacaaa agtaagattt agcctgatac ggaacccgtc
     7981 acgtattttt tcagtggaaa aggtgcgttc tgatatggct ttaacacgat ttaaacaaaa
     8041 ttgcctattc tgtgcatcgt aattccgcat aacaccgtat tttgcgccct caaatcgaaa
     8101 tcatgaaacg tttaattttt gtttttgcca tggctgcgct tgtagcttgt ggatctaaga
     8161 atccgatgga agaatcggta aatgtggaga aaaaggaaga taagctttct tatgccatgg
     8221 gagtttatat tggtcaggac atcgccaaaa tgctgaaaat gcaaggtcag gattccatca
     8281 tcgataaatc attgctagtt cgcggattgt acgatgctat tcttgaaaaa gaaactgcta
     8341 ttcctgccga atcggccaaa caggtagtgt ctgatttcct tcgtgagcag gatgaagatc
     8401 gccgcaaagc acaaatggct cagttcgagg gaatacgtca ggaaggtgaa aatttcctcg
     8461 ctgccaataa agaaaaagca ggtgtaacca caacgctttc aggattgcaa tacgaagtca
     8521 tgaaagcagg taaaggtccg aacccaaagc tgggtgattc agttgaagtt cattatgtag
     8581 gtacactact caacggaacc aaattcgaca gctcggtaga tcgcggcgaa accttcaagt
     8641 ttgaagtggc ttacggcggc ttgatcgaag gatggatcga aggtgtacag ctgatgaacg
     8701 aaggtgcaaa attcagattc tggttgcctt atggacttgc atacggtgat cgtggagcag
     8761 gacaagatat cccgccattt tctgcactcg tgtttgaaat tgagttgatc aagctgactc
     8821 caaaaaaata acccaaaaat cccgggtttt aacccgagga tttttaccat atatcaaatt
     8881 gtaaccccgg gcttcagtcc ggggttattc ataatggtac ctctgaactt atacttctaa
     8941 tcgtatacgc tggcttcagt ccggggttat tcataatgga tccttcttcc tgcaatcaca
     9001 tcctcctatg ttctgcacag gtggaatttt aattctcttt aaaaaccagt gcgactccgt
     9061 tgatgcaata tcttaaacct gttggacgag gaccatcttt gaatacatgt ccgaggtgac
     9121 catcgcattt tgcacaaagt acttccgttt cgatgctacc gtaagatcgg tcttctgctt
     9181 cacttactga attagagaaa gcgggttgaa aaaaagaagg ccagccggta cccgaatcgt
     9241 atttttcaga tgagcggaac agcaactgat cgcaacccgc acaatggtaa ttccccttac
     9301 gtttgctgtt gtgaaaagaa ctggtgaagg aacgttctgt ccctttttta cgcaacacat
     9361 aataggcctc tgcgcttaat cgctcgcgcc attgggtatc ggtgagcacc agctttttca
     9421 gtgtggtatc cgtttgcgga atgcttagat ttgcctgttc gtttacagga ctagtacacg
     9481 agctttgcag catccaggag ctgaagaaaa tgaagatggg gagaagtgtt tgcatgcaac
     9541 aataacgcaa aaaacatatg aatcgttcaa gcgctttaac aatttttaat ctcgcctcgg
     9601 atagtgtcga ttcggaagag atcagggatg cttacgaaca ggccgttttt gagatcagca
     9661 accgtttttt gcgtaatcat ccccaaccct ttctggttcg tgctaaaata aaaagattgc
     9721 aaactttctc agaagcatgg ttggtactaa caggccaaag ccccggacca ttaccggatc
     9781 ttcccgaacg gaagctggat ttgggtggac tggaaccttt gttgcgtagt tacgaagccc
     9841 atttggcagc actccgaacc gagttggcta aatgcaggga agcgcttgcc gcagcagcag
     9901 ttatggaacg aatggcagaa ttgcaggagc aatgggacaa ggaactggtg cgtcgtttcc
     9961 ccttggaaga agatcaatgg aaagcggtga gtgtgcagca gatgccggat accgttgcca
    10021 tgatgaaagt gatcagtgaa aaaggtgaag agcaggtgat ggccggggaa agtgtacatt
    10081 tgcaacagcc ttttatcagt gaaagtgcgc gcatacaccg acaaaacagc agagcgaact
    10141 aatggaagat gatcgtttgg taaaactcag ggaattgctc acggaaaatt tccgtcctcc
    10201 tgaaatttat atgtttaagt ttatagtacc caataataat caggcacatg caatggcaat
    10261 ggctttgttc gatgagtccg ctcaggtgga gtcgcgtgtt tcgggtaagg gttcctacat
    10321 ttctcttacc gcccgggaaa tgatggtttc ggtggattcg atcctcgaaa aatacagggc
    10381 ggcttatgct attgatggct tgatcgcact ctgatatgac ctctgcattt tttattgttt
    10441 tgttgtattc gctcaccgta accgtgggtg tttttctcat gttttttggc taccgttaca
    10501 tcatcaaccg catgagtggc aaacacagcg gtagaaaata tgtgaaagtc cgccgtatcc
    10561 acgaaggacc attctccggg gagagttttc tggttttcga tctgccggaa caggctgcca
    10621 ttcgctttgc catacaggat aacacagagc aggaaattgc cgttttactc gaggaagaat
    10681 gccgcatggg agaactggta atccacgtgg atactacaaa attccccaac ggacagtatt
    10741 atttcgaact aattgctccg ggtcaacgta ttcagcgtaa attcagcatc agtaacgtat
    10801 gaaaaaagcg ctcatcggat tcttattatt cgcttttcca tttgtgctca ctgcgcagga
    10861 agagggagaa acttcgggta atgtacgccc tgaaaagatt cccgatatcc gctacggttt
    10921 caaaggatcg ttcaaattgc ccacgcccat taagaacaaa gcattcactt cggtgatcga
    10981 tggaattgct gatgccaatc tttcttttca ctatccgttt ttgcgtcatt tttattttgg
    11041 tgccggttat cgctattcgt attttcaaat caacgatttc cgggcaaagg ctgatctcaa
    11101 tgcgaacctg caaattcatt ctcccttcgc agtactcggt ttcgaacgtt tcgccacacc
    11161 tcgttttctc tatggcgttt cattgcgctc aggctattcc atgctgcatt tcaaaagcaa
    11221 tacttgtctc agcaacaatg ccgaaggtaa aggtgccgga caggaagccg ttttcatcga
    11281 gcccgaattt acacttgagc tgatgtcggg cgaaaacatg gccttttcct ttgttacatc
    11341 ctggacctgg attctcgatg aatttggcgc ggagaatatc tgcctgagta atttttccgg
    11401 actcactgcc gatgatagca aagggtatta tcagtttatc tcggttggat tcggttttac
    11461 ggtcttcctt ggtggcgaaa agcgcagcaa aggtccgcgg atctattacg attaggctgc
    11521 tgttccaaac gcaatctgcg ttgtgtaaaa tgcaaccctt aattttagct taacattccc
    11581 tacaaactcc cactgtactt tcatggccgg taagaaggaa aaaaaggtga aaaaatctga
    11641 aaagttcgag gtcatcaacc gcgacctgag ttggttgtcg ttcaatgcac gcgtattgca
    11701 ggaagccgac gacgatcgtg tgccattggt ggagcgaatg cgtttccttg gaattttttc
    11761 caataacctt gatgagtttt tccgtgtacg tgtagcaaca ctgcgcagga tgggagtact
    11821 cgataccaat gcaaaaaaag aattcggtta cgatccgaaa aaaattcttg atcgaataca
    11881 aaagacggtt gtcgaacagc agaaggagtt cgaacgcatt tttcaggaga tcattcgaaa
    11941 actggaaagc aaaggcattg ttctgctcaa tgaaaagcaa ctgagcaagg aacaaggtgc
    12001 ttttgtgcgg aattattttc agacaacagt tcgtcagacc ctggttccca tcatgctcga
    12061 taaaaagagc aaaatgctca acctgaaaga caaaatgatt tacctggcta tcaagctgag
    12121 ccgcagtacc gatcgcagcg atttcaggta tgcactcatt gaggttccaa cgcaggttgt
    12181 atcacgattt cttgttcttc ctcaaggaaa aagcaataag accaatatcc ttcttctcga
    12241 tgatgtagtg aggttttgtc tcgacgacgt attccagatt ttcgattacg atgtactgga
    12301 agcctatacg ataaaactta cacgcgatgc agagctggat attgatgacg atatgcagca
    12361 gggaatggtg gagaagattc agcgcagttt gaaaaaacgg aaactgggtc agctggtgcg
    12421 atttatttac gatgccgaaa ttccgccgga tctgctcatc ttcattacac gtaaactgaa
    12481 actggaaaac aataaaaacc tcattgcagg aggacgatat cacaatttca aggattttat
    12541 caaattcccc ggtattggtg atgcttcctt gcaaaacgaa gaactgccgc cactgacgca
    12601 tcctgatttg atcaatcaac gcagtattct tgatgtgatc cgcaaaaaag atgtgttgct
    12661 tacttttccc tatcaatcgt tcaactatat catcgaattg ttgcgcgaag cagcgatcga
    12721 tcccaaggtg gaacatattc aaatcaatct gtaccgagta ggcgaaaaat cgcgggtggt
    12781 taatgcactg ctcaatgccg ccaaaaacgg aaagcaagtc actgcaattg tagaattaca
    12841 ggcgcgtttt gatgaagaga acaatatcaa actctcacgc aagttgcagg atgaaggagt
    12901 gaaagtgatt ttcggcgttc cgggattaaa agtgcattcc aaactgctgc tcatccaccg
    12961 aaaagaacgc aaaaaggaag ttttgtacgc acatgtggga acaggcaatt tccacgaggt
    13021 gaattcgcgg atctataccg accatgccct gctcacagca gacgaacgta ttaccactga
    13081 ggtggaaaaa gtgttcgagt tctttttacg caattacgaa cgcgccaatt ttaaatcgtt
    13141 gtttgtttcg ccctttaata cccgaaaacg atttatcgag ctcattcagc aggaaattca
    13201 caacgccaaa cgtcaccacc cggcctacat tacgcttaag atgaataatc tggtcgacga
    13261 agaaatgatt cgctggctgt accgcgcttc gcaggcaggt gtgaaaataa aaatcattgt
    13321 ccgcggtatc tgctccctca tacccggtgt ttcaggaatg agtgaaaaca tcgaagccgt
    13381 gagtattgtg gatcgttttc tggaacatgg acgcgtattt attttctgca acgacggcaa
    13441 agaactcgtg tacatttcct ccgccgactg gatgcaacgc aacctcgatc atcgcataga
    13501 agtaagtacg ccaattctcg atacacaggt taaaaagcag gtactggata tgatcaatat
    13561 ccaactcaaa ggtaccgcaa aatcccgcat catcgaccgc gaacaaacca accgctacaa
    13621 aggtgaaggc aaaaaagatc tgttacgttc gcaaattgag atctacaatt atttgaagaa
    13681 actgaaaggg taatttccaa ttatctgatt gctcaatttc ccgcacctgc cctatggttt
    13741 gtcctcctgc tagccttccc cggtgaaaca taggcggaag aaggggaatt cactcactta
    13801 cgcattcgca cactccgtac tagcacatcc gcacacttgt ccgtcgtcct gtccgccgta
    13861 gtgaaaggta ggaggaaaca cagtgaaggc ggattcccct attctcacat tcccccacta
    13921 gcacattctc ccactagcac atttgcacat tccattcccc cactagcaca ttcccccact
    13981 ccctcattcg caaattcgat caaggttttc cacaatttag gccacaacgt gaacaactta
    14041 ggcttaaatc gaaggtgctc cttggctgtt ctttctattt ttaagccaaa attaaaacat
    14101 tgatttacgg agcaattgac ataggatcca atgcagggcg tctgctgata ggcaatgtgg
    14161 tggaagaaga cggaatggca tcgatcaaga aattttcgct cacacgtgtg cccttgcgtt
    14221 tgggggagga tgtgtttacg gataaaaaaa tcagtccggc cagggtgaaa atgctcatca
    14281 aaacactcaa ggcgtacaaa catctcatgg atgtatacga tatcgttgat taccgcgcct
    14341 gtgccacttc tgctatgcgg gaggcggaaa acaggaagga gattctggaa gagattaaaa
    14401 aagaaaccgg aataaaactc gaaatcattg ccggggagga agaagctgaa ttgatcttct
    14461 ctaccatcaa caccatgaaa ttcgagagga ataaatctta cctctacatc gatgtgggtg
    14521 gaggaagcac cgaagtgagt ctgctcgaaa acggagagcg caaaaaatcg aaatccttta
    14581 aaataggaac actgcgtatc ctcaagggaa aagtggatga aaaacgctgg ctcgaactca
    14641 aagactgggt gaaaacactc aatccggaac acaaacagat cctggctatc ggtacaggtg
    14701 gaaacataaa ccgcatgctc aaaatcaacc gcagtgagga cgataacagc atcagctatt
    14761 ccgatatcaa acgcatccag acccatattg cctcgtatag tctcaacgat cgtatcaata
    14821 aacttggttt gcgtcccgac cgtgccgatg ttattgttcc cgcagccgat atttaccttg
    14881 ctgtaatgcg ttatgcagat gttacacaaa tgctggtgcc taaagttgga ttgtcggatg
    14941 gaatcattta tgaattgtat aagaagaaca ggaagaagtg aaggtgaata gttaggtgat
    15001 cggtgaatag tgaaaagtga atagtgaagg gttgtcgatg gctggattga gccggtagtg
    15061 aggttttgat gtgaggtaat taataatcac aaaatcaata taacatgcag catccgttta
    15121 gacaggagaa atctattttt gatggtcaat ttttattgat tactgaggat aatgaattta
    15181 ggatgagttt gtgggttaaa gggtttggca tccatagtgc aaatggagaa gaggtgctgc
    15241 ccttagaaag ttcttttaat ttgattcggt atgaagaatc ggggaatagt ttaattgtcc
    15301 attttgatgt ttaccctaat ggaagaaaac aatacgatgc agaaataaat ctatttaata
    15361 gaacgtttac atacaataag caaacaatgg gtttggataa atttgttgga acgtttatga
    15421 gtattcagga ttagagattg agaatgattt aaatttgtca attaactttt atgaattttt
    15481 gttcattgta atcaaaaaaa acctcaatgt cgataagcaa tgaagatgaa ctgctaggcg
    15541 tgcaaaaggt gagtgatgtt gttgcgctca ccttgcggga aatgaaagca tttgctcgcc
    15601 ccggtatgag tacaaaacag ctggatgatt ttggtgctgc attgttgaat gaatttggcg
    15661 ctcattccgc acctctgaaa acctatgatt ttccgggctg tacttgtata agcgtcaaca
    15721 gtgagttttg tcacggaatt ccttcgatac accgcatttt gcaggaaggc gatctgatca
    15781 atattgatgt ctctgccgag ctcgatgggt attggtcaga taatggagca tcttttgttc
    15841 ttggtgacga ttttcatggt catcagcaat tggtggatgc ttccaaagag attcttcagc
    15901 gagccatcaa ttccattcgg ggaggtttaa aaattgccga tctgggtcat cttatcgaaa
    15961 cggaagcgaa aaaacggggg tacaaagtca tacgcaacct taccggacat ggcattggca
    16021 gaagtctgca cgaatctccg gcagagattg cgaatttcag agacaaatac aatcataacc
    16081 gattcaagaa aaatgctgta gtggccattg agacgtttat ttctacgcat tcggatattg
    16141 cagtaacact aaaagacgga tggacgatgg tagggaataa aggtggtttt atggcgcaac
    16201 acgaacacac tattgttgtg actgatgtcg aacccattat cttaacctgg aataatggga
    16261 tttggtgaaa agggcaatag tttgaagttt cgggtttagt tttattttcg ggttgtccct
    16321 gaggttgttc gtattttcat taaattgcct gaaggcggaa atactatgag gtaggtcagt
    16381 ttgagttttc aatagtaatc gtgtcatcgg ttgctattgt tgtggtacat ggttattgct
    16441 caaagtttgc aattaatttt ttaggcatga tcaaattggt tactatacct gataaggcca
    16501 aagaatacca gaaatatatg gtatttgtaa tcacagtaat ctggactgtt gtaattggtg
    16561 taattgtttc tttgggtttt attttttttc cggatatctg gcaacgttat ctgacctttt
    16621 tatttgtttc aattctgatt ggtttcgcga gtttgctatt gatccgcaat ggacatgtta
    16681 agtcggcaag ctggttcctt ccgattgtat tgtggatatt tataacaatt ccctgcaatt
    16741 ctgcaggagg aatattggca ccaggaataa tgactcagat gagtttaata cttacagcag
    16801 ggttattatt gggttttcga ggtggattat tcattggact actaactata agtactgatt
    16861 tctattttgt gtatgccgaa atcaatgggt ttttacctga gccaacggtt atgcatactc
    16921 caatgaccag atggatcagc aatattattc ctttcgggac tattctggcc ttacagtatt
    16981 attcaaccaa ccatttgcga agtagtttag ttgctactca gcgggaagtt gcacttcggg
    17041 aagaggcgga acgagtgctt aaacagacgg ttgataactt gaaagagcgg gtaaaagagc
    17101 aaaaaacttt ataccgttta agtcaggttt tgcgtaatga agaagtagga atccctgaac
    17161 tcacaaagca aattgtagaa actctacctg ctggttggca gtttccggat tatttaaagg
    17221 caagagtcag aatcgatgaa atagaatttt cttcaagcgg atttgaagaa agcgaacagc
    17281 gacaatacgt taaattaaat accagttccg gaactacagt agttattgag gtcgtgtatg
    17341 cttcgggtgt tccttgtaag gatccgcaac tttttttaaa ggaagaagaa cacttgataa
    17401 agatgtgtgc agaaatgatt aaaataagta tggactcgcg ggaaaataaa aaagaactgg
    17461 aagaatataa atatgcgttg gatataggtt caattgttag tattgctgat gtaaatggta
    17521 taatgacatt tgtaaacgat aatttttgca agatcagtaa gtacgaagag catgaattaa
    17581 ttggaaaaca ttacggtatt atatcatcag agaatcattc agacgatttt tttaaaggac
    17641 ttgcaaatga aatgaagaaa ggaaagccat acagaggcga gttttgcaac cgtgcaaagg
    17701 atggttctgt ttattgggtc aacacttcaa ttgttccgtt tttagatcag aacggaaaaa
    17761 tttaccagta cttgtccatc aaccatgata taactgaccg aaaaaatgca gatgaaaaaa
    17821 ttaaggaaag tgaaaaactg cttaaaaaga taactagcca ggttcccgga aatacttata
    17881 tgtttgaaat tgaggagtca ggtaaagcta atctattatt cgttagcaga ggcacagatt
    17941 tgtttaatca tcaatatagt ttggaagaat taaatgaaca tccggaaagg ttgcaggagg
    18001 ttttgtttga agaggataag gaaaaattta atgatgcaat gattgatgcg cataaaacac
    18061 gcttaccaat caactttcaa taccggattg ttatcaatgg gcatatcagg tggcgttgga
    18121 tgcaggcaat tcctgaagtg gatacaaaag gaagggtgtt gtggtacggt gcaacaagtg
    18181 atattacgcc actagttgat tacattacct ctatagaaca gattattttt gatatcagcc
    18241 atgtccttcg aagaccggtt gcttccatga aaggaatgtc gaaactt
//
LOCUS       DAOMYG010000008        18164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1056_length_18164_cov_9.941963, whole genome shotgun sequence.
ACCESSION   DAOMYG010000008 DAOMYG010000000
VERSION     DAOMYG010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 18164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18164
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1056_length_18164_cov_9.941963"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="PK610_00630"
     CDS             <1..438
                     /locus_tag="PK610_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34457.1"
                     /translation="AEERSPFLERNAPRFLLISSSLYFLSLLFLSYSFILKGVYTFTL
                     ISLFFLASIIVYSAVCTNTGKKILILFVLFPFYCICLLSLFFGRKMDEFKVNDNHKII
                     LSSGGMLSAGEELTVVRSGYFIFDKKLTDINNICLAEIQDTEN"
     gene            complement(575..2029)
                     /gene="bchE"
                     /locus_tag="PK610_00635"
     CDS             complement(575..2029)
                     /gene="bchE"
                     /locus_tag="PK610_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-protoporphyrin IX monomethyl ester
                     anaerobic oxidative cyclase"
                     /protein_id="HRJ34458.1"
                     /translation="MKILLVNPPHLSIGSRMPKEHLPPLGLLSIGGPLIDAGNEVQLL
                     DADFYNYKIELIVSKIKDASPDMVLLGHSGSTSAQPIIEEITRKVKAIAPQIITIIGG
                     VFPTYHWKEILEDQPHIDFIVCGEGEQVILDLTKCIAQQNDPETVKGIAYQRDGIVVK
                     TSAAPTLQNLDDYRIGWELMKGYQYTYWGKRKAVVVQFSRGCPYPCTYCGQSLFWKKW
                     RHRSPQSLADEMEMLHRNYGIEVFNFADENPSSNPKAWRAFLEALIVKKMKIILVGSI
                     RADNIVRDAEFLHLYKQAGFERFLLGIENYDEVVLEKIKKAGTVSKDKEAIRLLRQHN
                     ILSMATYVVGFGEERTRDFYNSLKQLLDYDPDQIQLLYATPHKWTPYFEEVKDKTIIL
                     TDQRKWDYKHQVLATEHLQPWHVILYVKLIEIIMQTRPKAIKRLLFHKEKRLRSAMRW
                     YTRIGRRVWFWELWQFFFVTPLNYQKVKLREFWR"
     gene            2890..3558
                     /locus_tag="PK610_00640"
     CDS             2890..3558
                     /locus_tag="PK610_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRJ34459.1"
                     /translation="MNALVLLAMLSPMGPVLQNQDLLTMEIAKENENKNGNAYQDSLY
                     DFTSFDECAEEFGHADQWVCEDELLELGGYDGRNSKKVVKIRRMWLREKTYNLVFNFS
                     RIISGHQPLIQGLRLNRAAWKHSKWMAEQNRGINHRDFYDRSERYNFDAENVAGDYSS
                     IFGALFGWINSKGHRKNMYAYSHLFHGTGFAKRVEIYYVKNKKGRWVRDGKKKTKFYT
                     QVFR"
     gene            3618..4325
                     /locus_tag="PK610_00645"
     CDS             3618..4325
                     /locus_tag="PK610_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34460.1"
                     /translation="MLWRRWMFFVLVTMPFLLSACYCEKVTYGRYDVRFLNTYKEKTY
                     CYPGFTESATFFVMPYVGVLDQSHIFQVHRMYPGEEILALHSQSSPQIGLRMGFFPRV
                     FFLPGPFLKLGCDISYTETELSVPRLIDHLPFDHQAQLFQYRILLHAQLATWVKPRFM
                     GSITTQYGVNFADEQYQLKKSFSSSVEQSGRKLHVLQNMRLGYAFNFGLRKGLAFSME
                     VGIGAGTFLRSGLMFYF"
     gene            4381..5397
                     /gene="selD"
                     /locus_tag="PK610_00650"
     CDS             4381..5397
                     /gene="selD"
                     /locus_tag="PK610_00650"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRJ34461.1"
                     /translation="MEEIRLTQFSKGSGCGCKIAPSVLQEILGQHKLSPDGGKVLVGN
                     NSADDAAVYELENGQCLISTTDFFTPIVDDAFTFGQIAAANALSDVWAMGGDPVFALA
                     ILGWPVGKLPTELASRIMEGARSKCAEVGVVIAGGHSIDIPEPVFGLSVNGFVQKTYL
                     KRNSTPQHNDVLFISKPLGIGILSTAQKRGLLQSDEYAVLLQAITALNTQGAQWGKLT
                     EVHAMTDITGFGLIGHLLEMLAETPLSAELNYTAIPLLDPVKKYTAQFVYPDNTMRNW
                     SAFQGDVEGINGESLLTLCDPQTNGGLLVAVDADAADHFEQSFPVFRIGKIIASGEKK
                     ILIR"
     gene            complement(5408..6418)
                     /gene="mnmH"
                     /locus_tag="PK610_00655"
     CDS             complement(5408..6418)
                     /gene="mnmH"
                     /locus_tag="PK610_00655"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HRJ34462.1"
                     /translation="MERISIDRFLEIREQYPVIDVRSPGEHLQGRIPGAVNIPLMENE
                     ERAVVGTMYKQNGKQAAVLKGLEIAGPKLKDYIKTARKIPNEGTFIVHCWRGGMRSEF
                     FSWLLEFYGFKVMVIKGGYKSFRRKVIDEFNCDRSLMVLGGKTGCGKTKILHELRRKG
                     EAVVDLEGIAHHKGSSYGGLGEEPQPTQEQFENELGIQLLSIDTSKNLWLEDESRVIG
                     NKVIPEGLWLQMKKAVLHVLELPFEQRLQTILDDYGKFPAEALIEATLRIGKRLGPEQ
                     TKNAVAALREGDLKTGFTYALEYYDKSYQHKINERGNNNLKIHRFDEWSAPTIIAELL
                     AE"
     gene            complement(6420..7592)
                     /gene="purT"
                     /locus_tag="PK610_00660"
     CDS             complement(6420..7592)
                     /gene="purT"
                     /locus_tag="PK610_00660"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HRJ34463.1"
                     /translation="MTKKILLLGSGELGKEFVIACKRLGQYVIAVDSYNDAPAMQVAD
                     EREVINMLDGDALDRIVAKHNPDLIVPEIEAIRTERFYDYEKQGKQVVPSARAANFTM
                     NRKAIRDLAAKELGLRTAKYAYATSENELRNAIQSIGLPCVIKPLMSSSGKGQSVART
                     ESDIANSWKYAVEGSRGDIVEVIVEEFIPFDYEITLLTVTQKNGKTLFCPPIGHRQER
                     GDYQESWQPCVMNSEHLREAEQMAEAVTRSLTGWGIWGVEFFISEKNGVYFSELSPRP
                     HDTGMVTLAGTQNYSEFELHARAVLGLPIPEITLERCGASAVVLAKEEGKNPTYIGVE
                     KILDHFNTDVRIFGKPTTRPYRRMAVVLTHDKPGSDVGAVKEKAKKLAAGIQVKSL"
     gene            complement(7598..9043)
                     /locus_tag="PK610_00665"
     CDS             complement(7598..9043)
                     /locus_tag="PK610_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HRJ34464.1"
                     /translation="MEKIANYINGELLAPVAGKYIDNYNPALGEVYSLIPDSDEQDIE
                     NAYKAAAAAAQAWGNTQAEERFRILNRIADLIDRDLDQLAMAESTDNGKPVKLAKTVD
                     IPRASSNMRFFATSILHFANEAHGMEDRGFNYTLRQPIGVVGCISPWNLPLYLFTWKI
                     APALATGNAVVAKPSEVTPYTAYLFSKLCIEAGLPKGVLNIVHGLGGKVGSAITKHPR
                     IKAISFTGGTVTGAEIARVAAPMFKKLSLELGGKNPNIIFADCDFDKMMSTTMRSSFA
                     NQGQICLCGSRIYVQRPIYEKFRDEFVKRTEAMRVVDPSDEKSQMGAIVSQGHIDKVM
                     SYIELAKEEGGKVIAGGTRVQMDGKFSKGYFMRPTIIEGLPHTCRTNQEEIFGPIVTI
                     QPFDTEEEALEYANSTQYGLAATVWTQDVNKAHRVATKLESGIVWINCWLVRDLRTPF
                     GGVKSSGVGREGGFEALRFFTEAKNVCVNLS"
     gene            9422..11038
                     /locus_tag="PK610_00670"
     CDS             9422..11038
                     /locus_tag="PK610_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRJ34465.1"
                     /translation="MIFGRHIRMVIALIAIAVVGLVGVQIYWIDNAITLRTAEFKENV
                     NAALLKTVESLQRNEALGYIRNTALGSRFFSGVDRGRMMNAQTDCMTTRDTVMMKDGK
                     EVRMQVLEKQSSDSMLGIRTEERVIGSVVQNGFDEKTGEASLNLVFDDSIRLFFKPRT
                     LGDQMVGEKAQMVEEILSEMFSFRGFMPIGLRVDVKELDSLLKVSLHHYGIFAPYQFV
                     LTDIEHRVLLTSDSVSSEMKPVILNEGYSINLFPGDFFNEPVFLFVHFPSQHRYLLGR
                     MWSILSLSGVLILIIIFAFYFTISTILRQKKLSEIKNDFISNMTHELKTPISTIALAS
                     EMLSDQAVQLADDRRTDFIRMIREENKRLGILVENVLQTAVIDRGELTLKKELFDFND
                     LIDDVAGNFSLQINQRGGKLTTKHSDGPCAIFGDRVHLRHVLYNLLDNANKYSPEAPE
                     ITMLSNTNNERVQVAISDRGVGISKEDQKRIFEHLYRVPSGNIHNVKGFGLGLSYVKA
                     VVEKHGGTVSVSSDPGKGSTFTIEIPLRHD"
     gene            11031..11720
                     /locus_tag="PK610_00675"
     CDS             11031..11720
                     /locus_tag="PK610_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ34466.1"
                     /translation="MTNTTNILLVEDDPNLGTLLKEYLNAKGFPTTLCTNGQLGFEAF
                     CKGKFDFLILDVMMPVKDGFTLAKEIRKLNTKVPILFLTAKSMKEDTLEGFNAGGDDY
                     MTKPFSMDELMARMSAILRRINGDKKVLPEIVQIGIYSYDSNRQELKSGDQSQKLTTK
                     ENELMKLLAGNINGLVERNFALTAVWGDDSYFNGRSMDVYITKLRKYLNADPNVEIMN
                     IHGKGFRLVVK"
     gene            complement(11801..12901)
                     /gene="dprA"
                     /locus_tag="PK610_00680"
     CDS             complement(11801..12901)
                     /gene="dprA"
                     /locus_tag="PK610_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRJ34467.1"
                     /translation="MDSDERLFYTIGLGLLEGIGPVNARKLIAFAGSAEQVFKQKRHQ
                     LMRTPGVGEVIASAIHTGVLERAEKEMEFIRRNKIRACTFLDSAFPQRLKNCEDAPLV
                     LFSLGNADLNTSRVVSIVGTRLPSEKGKEICTSLVENLAGKGITVVSGLAYGIDICAH
                     RSSLQHAIPTVGVLAHGLDRIYPGAHRSTAEKMLEQGGLLTEFISETNPDRENFPKRN
                     RIVAGMSDATIVIETGNKGGSIITAMLANDYNRDVFAFPGRPGDEKSSGCNRLIKIHR
                     AALVENANDILLTMGWEENNSKKNAIQIPLFPDLDPDEKIVFDLLQENNQTGIDALCL
                     QSNWPMSKVSALLLTMEFKGIVKSFPGKLYGV"
     gene            12958..15399
                     /locus_tag="PK610_00685"
     CDS             12958..15399
                     /locus_tag="PK610_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ34468.1"
                     /translation="MKTKFAVHKSAVFVLFALFISLVSKAQNATIRGKITDKSTKEAL
                     VGVNVVLSKGGGVASDINGQYKVSVDPGEVKISFRFIGYKQVDMTLQLKAGEDKVLDV
                     VLEEANTELETVVVSAGRFEQKLEDVTVSMNVIKPSLIENKNTLNCETIIDQVPGVSV
                     QDGQASIRGGSGFAYGAGSRVLLMVDDLPLLAADAGDVKWNTLPIENIEQIEVIKGAS
                     SVLFGSSALNGVINIRTAYPTDKPVTKIAITSGLYDNPRRDTARWWDKNPAYHGINFF
                     HSRKIDRFDLVVGGAAFSDKGYRMGEEEYRGRINFNTRYRSKIAGLSFGVNGNYQYSK
                     AGIYIIWKNANEIYVPSGYDPALGWDPSKNPNSTISINFGNRLNIDPYITYFAKNGDR
                     HALRTRVYWTRNVNNTNQSADAYMTYGEYQFSRRTKSDFNITAGLVGYYSKVLSQLYG
                     NHFGTNAGAFVQVDKKLNRWTLSAGMRGEYFRIDTAETVSRSSIGNLNINLPFKPVFR
                     AGANFRAREETYIRASVGQGYRFPTIAEKYVSTAVGALNIFPNPNLSAESGWSAELGI
                     KQGVKIGKWLGYADAALFVTQYTNMMEFAFGVYNPDSIPLNLNPNHPGYLTNWVGFRA
                     QNAEEARVSGIDLSIIGKGKIFKKIDMTVFAGYTYMRPITLNTDTNYLYSFSDAATIR
                     TIDGKDAMILKYRFQHMAKADVQFDYKKIGFGFSVRYNSNVYNIDRTFENLEVKIGGG
                     QLPLGDYILPGLPEYRKEHNRGACVADMRLAYTVAKGTRIAAVVNNVFNLEYMGRPGD
                     VQAPRTFALQAVVTF"
     gene            complement(15480..16382)
                     /locus_tag="PK610_00690"
     CDS             complement(15480..16382)
                     /locus_tag="PK610_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HRJ34469.1"
                     /translation="MEKILITGGSGMLGTRLTKRLLKEGYQVAHLGRSRNSRNPVKVW
                     LWDTRKGTIEAGALDGVDYIIHLAGEGIADGKWTEARKHSVLRSRIDGPALLFRKLKE
                     RNQPLKGFFSASGINYYNATSDNIHNEEDKPGEGFVSNVVIEWEKAAHQFDDLCRVVC
                     FRFGMLLDKNEGAIPRIGFPVQFGLGAAIGSGKQWTPWMHADDAARCFLFAIQNKSLN
                     GNFNAIAPQHLSNKELTKAIGRAVGMPVFLPNVPAFFIRLAFGEMSQLVLEGPRASSE
                     KLIQSGFTFLYPDIDSALSEIYLM"
     gene            16470..16865
                     /locus_tag="PK610_00695"
     CDS             16470..16865
                     /locus_tag="PK610_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34470.1"
                     /translation="MMYLRLLSASIVDNLPKSPSDLQPHSMDQEIIAATAQQVIKDFG
                     EFSFEIVFTGKGTSPYAELFEQVLPVVEHLLQTNTERFHALLYRIDVNESLIKRYSAE
                     TPGMRFPEMVTDLILRREMMKVLTRRYFR"
     gene            17054..17974
                     /locus_tag="PK610_00700"
     CDS             17054..17974
                     /locus_tag="PK610_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34471.1"
                     /translation="MKKIILLATLFLMGAGASVNAQYNACAAKNVITEKATETYVDQT
                     SGQTVTRTVERKVNADKHGNANGNQYDLAVDGAFDGQTIVVLHLYTGEGFDFSLPKAA
                     LQEKGFSVYRYMNQPPSPKELKEALDKACQLWVISTTSQLLNEEHAKVIKDFWDKGHG
                     VYLWGDNDPYHADANYLSRYLIGADMEGFYYAANVVTLKSDSTVSGLQRDHLITTGLE
                     YVYEGITIAKIHDHKNVLTPLIWSSDGNVVTSVYEKDGKRLILDGGFTRLYINWDTAG
                     TGRYVKNAAAWLVNYEKFGDAVVSTKVKSK"
BASE COUNT         4938 a         3904 c         4059 g         5263 t
ORIGIN      
        1 gctgaggagc ggtcaccttt tttggagcga aatgctccgc gctttttgct tatttcttcc
       61 tcattgtatt ttctttcttt gctgtttctc agttatagtt ttatactgaa aggtgtttat
      121 acttttacct taataagcct gtttttttta gcttcgatca tagtgtattc tgcggtttgt
      181 accaacaccg gaaaaaagat attgattctg tttgtgcttt tcccttttta ctgtatttgt
      241 ttgttgtcct tattttttgg gagaaagatg gacgaattca aagtgaacga caaccataaa
      301 ataatactaa gttcaggagg aatgttgagt gctggtgaag agcttacagt tgttcgatca
      361 gggtatttta tttttgataa gaaacttaca gatattaaca atatctgtct tgctgaaatt
      421 caggacactg aaaattgaag gaaagcgtgc agatgttttg atctaccata aggattattc
      481 cgaacctgaa aatccaaaac cattaagtgt ttacctgaat gaagaatggt aatccgcaat
      541 cagttccatg ttttatgaat tgcacaattg ctttttatct ccagaattcc ctgagtttta
      601 ctttctgata gtttaaggga gtaacaaaaa agaactgcca gagttcccaa aaccaaactc
      661 ttctcccaat gcgcgtatac catcgcatgg cactgcggag tcgtttctcc ttatgaaaca
      721 ataagcgttt tattgccttt ggccgtgttt gcatgatgat ctcaataagt tttacgtaga
      781 ggatcacatg ccagggttga agatgttctg tagccaaaac ctgatgtttg taatcccatt
      841 tgcgttggtc ggtgaggatg atggttttgt cttttacctc ctcaaagtag ggagtccatt
      901 tgtgcggtgt tgcatacaaa agttgaatct gatcaggatc gtaatcgagt aattgtttta
      961 aactgttgta aaaatcgcgc gttcgttctt cgccaaatcc aacaacataa gttgccatcg
     1021 ataaaatatt gtgttggcgc aataagcgga tggcttcctt gtctttggaa acagttcctg
     1081 cttttttaat tttttccaat acaacttcat catagttttc aataccaagc agaaaacgtt
     1141 caaatcctgc ctgtttgtag aggtgcaaaa attcagcatc ccgtacaata ttatcggcac
     1201 gaatggaacc taccaaaatg attttcattt ttttgacgat cagcgcctcc agaaaagctc
     1261 tccatgcttt aggattggaa gaaggatttt catcggcaaa attgaatact tcaattccat
     1321 agttcctgtg cagcatttcc atttcatctg ccagcgattg cggagaacgg tgacgccact
     1381 ttttccagaa aagactttgt ccgcaatacg tgcatggata cggacaacct ctcgaaaact
     1441 gaaccactac tgctttgcgt tttccccaat aggtatattg ataacctttc atcaactccc
     1501 atcctattct gtaatcatca agattctgca gggtaggagc agcgcttgtt tttacgacta
     1561 ttccatcgcg ttggtacgca attcctttta cggtttcagg atcattttgc tgagcgatac
     1621 attttgtaag atccaatatc acctgttcac cttcaccgca aacaataaaa tcgatgtgtg
     1681 gctgatcttc cagtatttct ttccagtggt aggttggaaa tactccgcca atgatggtaa
     1741 ttatttgtgg agcgatggct ttaactttac gtgtgatctc ttcaatgatc ggctgtgcag
     1801 aagtagaacc tgaatgtccg agcaaaacca tatcaggact ggcatctttt attttggaga
     1861 caataagctc aattttataa ttataaaaat ccgcatccag caattgcacc tcatttcccg
     1921 catcgatgag tggtccccca atggaaagga gtcctaatgg aggcaaatgt tctttaggca
     1981 tccgactgcc aatagaaaga tgtggaggat taaccaggag aattttcata gagatacgtt
     2041 cggttacttt cagaacttca caatctcatc gattagtatt tcagatttct caaacggcaa
     2101 tggttttatg tttttttaag aattgcaaaa acctcaccgc tggaatagaa tgcggtagag
     2161 tcagaaataa aagttaaccg caaagacaaa gtttcggatt atcgtggatc agaatccgca
     2221 ttcacccctt gtttaccaat accggaatat cttctcaagt ggagcaccct tcgactcccc
     2281 gacgttttcg gttggggcag gccgcccatg gaccttgaac ggtgaaaagt gaactgcaaa
     2341 gagaataggt tggtctgagg gaaattgaag tcttgtattt gatctgtaaa tacgaaacgc
     2401 tatatgatct tttttaagtg gagccatggc acctgcggtg cggcgcaaag gttatgccat
     2461 tggagtattg tgctaatggt tgttgatgga gtataattaa tgataccaac atagccatct
     2521 ctagcaggaa tttcagtggt atgccttcgc gttacgccga aggcgtctcc tatcacttga
     2581 aaaatacaac aacttcgtcc ctccctcccc attgtccgcc gtcttgtccg ccgaagtgaa
     2641 acgtaggagg attcgtggtt cccttccatt cacacattcc cacattcgca catggttcga
     2701 ccaaaggaga accatcccga ccgactgaaa ggagcgtcgg gattaacaaa ccaccacgta
     2761 ccctatctgc gttctaaaca ttttcttttt tgaaacccaa tcctgccctc tgcggtaaaa
     2821 ggagcaaatc tcttttgcat ggcataacgc tacgcttcta catcatgtct tactctaaaa
     2881 ccatgagcta tgaatgcgtt agtgttattg gcgatgctga gtccgatggg accggtgctg
     2941 cagaatcagg atctgttaac aatggagatt gctaaagaga acgagaacaa gaacgggaat
     3001 gcctatcagg actccttgta tgattttact tcttttgatg agtgtgcaga ggagtttggc
     3061 catgctgatc aatgggtgtg tgaagatgaa ttactcgaat tgggcggata cgatgggcgc
     3121 aattccaaaa aggtggtgaa gatccgtcgg atgtggctac gtgaaaagac ctacaatctg
     3181 gtgttcaact tttcgaggat cattagcgga catcaaccct tgatccaggg ccttcgcttg
     3241 aaccgtgctg catggaaaca ctccaaatgg atggccgaac agaatcgtgg tatcaatcac
     3301 cgcgacttct atgatagatc tgaacgctac aatttcgatg cagagaatgt ggcgggtgat
     3361 tacagcagca tttttggggc attgttcgga tggatcaatt caaaaggtca tcgcaaaaat
     3421 atgtatgcct acagtcatct tttccacggc accggttttg caaaacgtgt ggagatctac
     3481 tacgtaaaaa acaaaaaagg ccgctgggtc cgcgatggga agaagaaaac aaaattttat
     3541 actcaggtat ttcggtgatt cgttttagcc tgtaactttt cggctgttta cgtggttata
     3601 catgcaaatg gccgattatg ttgtggagac gatggatgtt ttttgtgctg gtaacgatgc
     3661 cttttttgct ttctgcctgt tactgcgaaa aggtaactta tggacggtat gatgttcggt
     3721 ttttgaatac gtacaaggaa aaaacgtact gctatcccgg ctttaccgaa agtgctacct
     3781 tttttgtgat gccttatgta ggtgttttag atcaatcaca tattttccag gtccaccgga
     3841 tgtatccggg agaagaaatt ctggcattgc attctcaatc ttctcctcaa attggattac
     3901 gaatgggttt cttcccccgt gttttttttc tgcctggtcc atttctgaaa ttaggttgtg
     3961 atatttctta tacagaaaca gagctttcag ttccacgcct cattgatcac ttgccttttg
     4021 atcatcaagc tcagcttttt cagtaccgta ttttactgca cgcacaactc gctacctggg
     4081 taaaaccacg ttttatggga agtattacta cgcagtacgg tgtgaatttc gctgatgaac
     4141 aataccaatt aaaaaaaagt ttttcatcat ctgttgagca gtccggcagg aaacttcatg
     4201 tgttgcagaa tatgcgcctt gggtatgcat ttaattttgg tttacgtaaa ggattggcgt
     4261 tttcgatgga agttggaatt ggagccggca cctttttgcg tagtggtttg atgttctatt
     4321 tctgaattgt aattaacgga gacgaatgca tgcgataaat cctctacctt tgtgtttacc
     4381 atggaagaaa ttcgtctcac tcaattcagt aaaggttcgg gttgcggttg taaaattgca
     4441 ccttctgtat tgcaggaaat tttaggtcag cacaaattat cgcccgatgg tggtaaagta
     4501 ctcgttggca acaacagcgc cgatgatgca gccgtttacg aattggaaaa cggtcaatgc
     4561 ttgatcagca ccaccgattt ttttacaccc attgttgatg atgcttttac gttcggacaa
     4621 attgctgctg ccaatgcatt gagtgatgtc tgggctatgg gaggagatcc tgtatttgca
     4681 ctcgcaatat taggttggcc ggttggaaaa ttacctaccg aactcgcctc acgcatcatg
     4741 gaaggcgcac gtagcaaatg cgcggaagtt ggggtagtca tcgccggcgg acattccatc
     4801 gatattcccg aaccggtatt tggattgagc gtaaacggat ttgtgcagaa aacgtattta
     4861 aaacgtaatt ctacaccgca acacaatgac gtgttattca ttagtaaacc acttggaatt
     4921 ggaattctct ccaccgcaca aaaacgtgga ctgctgcagt cggatgaata cgccgtttta
     4981 cttcaagcta tcactgcgct taacactcag ggggcacaat ggggtaaact caccgaagtg
     5041 catgccatga cagatatcac cggtttcgga ttaattggtc acctgcttga gatgctggca
     5101 gaaacaccat taagtgccga gttgaattat actgcaattc ctttattgga tccggtaaaa
     5161 aaatacaccg ctcaatttgt gtatcccgat aacaccatgc gcaattggag tgcgtttcag
     5221 ggagatgttg aaggcatcaa tggtgaatcg ctccttacgc tttgtgatcc gcaaaccaat
     5281 ggaggattgc ttgtagctgt tgatgcagat gcagcagatc actttgaaca atcgttcccg
     5341 gtatttcgca tcggaaaaat aattgcatcc ggcgaaaaga aaatcctgat tcgctaaacc
     5401 gaaagaatta ttcggcgagc agttctgcaa ttatggttgg cgccgaccat tcatcaaagc
     5461 gatgaatttt gaggttgttg tttccccgct cattgatttt gtgctggtaa cttttgtcgt
     5521 aatattccaa agcataggtg aatccggttt ttaaatcacc ttcacgtaat gcagcaacag
     5581 cgttttttgt ttgttccgga cccaatcgtt ttccaatgcg taaggtggct tctatgagcg
     5641 cttctgcagg aaattttccg taatcgtcga gaatggtttg caaacgttgc tcaaagggaa
     5701 gttccagtac atgcaacacc gcctttttca tttgcagcca caatccttcc ggaatgactt
     5761 tgttgccgat aacgcggctt tcatcttcaa gccataaatt tttagaggta tcaatggaaa
     5821 ggagctgtat ccccaattcg ttttcaaact gctcctgtgt tggttgtggt tcttctccca
     5881 aaccgccata ggaagatccc ttgtggtgtg caattccttc aagatcgaca acggcttctc
     5941 cttttcttcg caactcatgc agaattttcg ttttgccgca accggttttt cctccaagca
     6001 ccatcagcga acgatcgcaa ttgaattcat cgatcacctt cctgcgaaag gatttgtatc
     6061 cgcctttaat caccatcacc ttgaatccgt aaaactccag cagccaggaa aaaaattcgc
     6121 tgcgcattcc gcctctccag caatgaacga tgaatgttcc ttcgttggga attttccgtg
     6181 cagttttaat gtaatccttg agttttggtc cggcaatttc cagtcctttg agtacagccg
     6241 cctgttttcc attctgcttg tacattgttc ccaccacagc acgttcttcg ttttccatga
     6301 gcggaatatt caccgcccct ggaatacgtc cctgcaggtg ttcacccgga gatcgcacat
     6361 cgataacagg atactgctcc ctgatttcaa gaaaacgatc gatgctgatg cgttccatct
     6421 cagagcgatt tcacctgtat gcctgcagcc agtttttttg ctttttcttt cactgctcca
     6481 acatccgaac ccggtttgtc atgcgtaagc acaacggcca tgcggcggta cggacgcgta
     6541 gtgggttttc caaaaatgcg gacatccgta ttgaaatgat cgagaatttt ttccaccccg
     6601 atgtaagtag gattttttcc ttcttctttc gccaatacaa cggcacttgc accgcaacgt
     6661 tcgagtgtga tttccggaat gggcaagcca agtactgcac gtgcatgcaa ttcgaattcg
     6721 gaataatttt gtgttcctgc aagcgtgacc ataccggtgt cgtgcggacg aggagataat
     6781 tcagaaaagt aaacgccgtt tttttccgag ataaaaaatt caacacccca tattccccaa
     6841 ccagtgagtg aacgggtaac tgcttcggcc atttgttcgg cttcgcgcag gtgttctgaa
     6901 ttcataacac aaggctgcca gctttcctgg taatcgccgc gttcctgacg atgaccgata
     6961 ggaggacaga acaaagtctt tccgtttttt tgggtgacag tgagtagtgt aatttcataa
     7021 tcaaacggaa tgaattcttc cacaattact tccacaatat caccacgtga accttcaacg
     7081 gcatatttcc aggaattcgc aatatcgctt tcggtacgcg ctaccgactg accttttcca
     7141 gatgatgaca ttagtggttt gatcacgcaa ggaagtccga tggattgtat cgcattgcgt
     7201 aattcgtttt cggatgtagc ataagcatat ttcgctgtgc gtaatcccaa ttcttttgca
     7261 gcaagatcgc gaattgcttt tctgttcatc gtaaaatttg cagcccgggc agaaggaacg
     7321 acctgtttgc cctgtttttc atagtcgtaa aaacgttcgg tgcgaatcgc ttctatttcg
     7381 ggtacgataa gatcgggatt atgtttggcc acaatacgat ccagtgcatc accatcgagc
     7441 atattgatca cttcgcgttc atcggccact tgcatagcgg gagcatcgtt gtatgaatca
     7501 acagcgatca cgtattggcc gagtcgctta cacgcgatca caaattcttt tccgagttca
     7561 ccggatccga gaaggagtat ttttttggtc atggttatca ggataaattc acacaaacat
     7621 ttttcgcttc ggtgaagaaa cgtaacgctt caaatccgcc ttcgcgtcct acaccagaac
     7681 tttttacacc accaaacggt gtccgcagat cacgcaccag ccagcagttg atccacacaa
     7741 ttccgctttc gagttttgtg gctacacgat gtgctttgtt cacgtcttgt gtccatactg
     7801 tagcagcgag gccgtattgc gtgctgttgg cgtattccaa cgcttcttct tcggtatcaa
     7861 aaggctgaat ggttactatc ggaccaaaaa tttcttcctg gttcgttctg caggtatgcg
     7921 gcaatccttc gatgatggtg gggcgcataa aatatccctt actgaatttt ccatccattt
     7981 gcacacgtgt tcctcctgca attacttttc cgccttcttc ctttgccaat tcgatgtacg
     8041 acatcacctt gtcgatatgt ccttgagaaa cgatggctcc catttgcgat ttttcatcgg
     8101 aagggtcaac cacacgcatg gcttcggtgc gttttacaaa ttcatcgcgg aatttttcgt
     8161 agatcggacg ctgcacataa atgcgcgaac cacagagaca gatttgtcct tgattggcaa
     8221 atgaagagcg catggtggtg ctcatcatct tatcgaaatc gcaatcggcg aaaatgatgt
     8281 ttgggttttt ccctcccagc tcgagcgata atttcttgaa catcggtgca gctacacgcg
     8341 caatctctgc accggtaaca gttcctccgg taaaggaaat tgcttttatg cgtggatgtt
     8401 tcgtaatagc agaacctact tttcctccta agccgtgcac aatattcaac actcctttgg
     8461 gtaagccggc ttcgatacaa agtttggaaa agagataggc cgtataaggt gttacttctg
     8521 atggttttgc caccactgca tttcctgttg ccaatgcagg cgctattttc caggtgaaca
     8581 gatacagcgg aagattccac ggtgaaatgc aacccactac accaatgggc tgacgaagtg
     8641 tataattgaa accacggtct tccattccat gcgcctcgtt cgcaaagtga agaatagatg
     8701 tcgcaaaaaa gcgcatatta ctcgatgcac gcggaatatc tacggtcttt gcaagtttta
     8761 ccggcttgcc gttatcggtc gattctgcca tggccagttg atccagatca cgatcgatca
     8821 aatccgcaat gcggttaagg attcggaatc gttcttctgc ctgagtgttc ccccaggcct
     8881 gggcagcagc tgcggccgct ttgtatgcgt tttcaatatc ctgctcatcc gaatcgggaa
     8941 tcagcgaata cacttctccc aatgcaggat tgtaattgtc gatatacttc cccgccacag
     9001 gagcaagaag ttctccgtta atgtaattgg caatcttttc cattgcaacg aaagtaggaa
     9061 attttgaagc ctttttggag gaaaacactc taaaatgaca gggatcagag actcagatta
     9121 ttcccctaaa aaagaaagcc ccccggtttc ccgggaggct tccctctctc ttgttgcgat
     9181 atgaacatcc aactctcgtt gaacgtatgg gaaaagaact cgaatcaata acgcaaaggt
     9241 atatgccgca gtatttttga aggaaaatat tcttccgacg gtaaagtcgt gcgataaatg
     9301 gtactttgac agtacaaacg tagcttgtta ctgctcagtg cgctgttaag ggaatgctaa
     9361 aaggtgttaa aatctgttaa gccatactac agggcagata agcgagttaa atttgttcag
     9421 catgatcttc ggtcgacata tccgcatggt aattgccctc atcgccatcg cggtggttgg
     9481 tctggttggt gttcagattt actggatcga taatgccatt acccttcgca cggctgagtt
     9541 taaagagaat gttaacgctg cactgctcaa aaccgtagaa tcgcttcagc ggaatgaagc
     9601 tttgggttat atccgcaata ctgcattggg ttcgcgtttt ttttcgggag ttgaccgtgg
     9661 cagaatgatg aatgcccaga cagattgtat gactacccgc gataccgtga tgatgaaaga
     9721 cggaaaggaa gtacgaatgc aggtactcga aaaacaatct tctgatagta tgctgggaat
     9781 tcgcactgaa gagcgagtta taggaagtgt ggtacaaaac ggttttgatg aaaaaacagg
     9841 tgaagcatct ttgaatcttg tttttgatga cagcatacgg ctttttttta aaccgcgtac
     9901 acttggtgat cagatggtag gagaaaaagc tcagatggtg gaggaaatcc tctctgagat
     9961 gttttctttc cggggtttta tgccaatcgg attaagagtg gatgttaaag aacttgattc
    10021 gctgctgaaa gtttcattac accattacgg aatttttgcg ccttaccaat ttgttcttac
    10081 tgatatcgaa caccgtgttc tgcttaccag tgatagcgtg agtagtgaaa tgaaaccggt
    10141 aattctgaat gaaggataca gcatcaatct gtttccgggt gattttttca atgaacctgt
    10201 ttttttattc gttcattttc cttcacaaca ccgctacctg ttaggacgca tgtggagtat
    10261 tttatcgctc tcgggtgtgt tgatactgat catcatcttc gcgttttact ttaccatttc
    10321 aaccatactg cggcagaaaa aactttcgga gatcaaaaat gatttcatct ccaatatgac
    10381 acacgaatta aaaacaccca tctccaccat tgcattggct tccgaaatgc tgtcggatca
    10441 ggccgtgcag cttgcagatg atcgtcgtac cgattttatc cgcatgattc gtgaagagaa
    10501 taaacgtctg ggtatactag tagaaaacgt attgcaaacc gctgtaattg atcgtgggga
    10561 gttaacctta aagaaagagt tgtttgattt taatgatctg attgatgatg ttgctggaaa
    10621 tttttcgctt cagattaatc aaaggggagg aaaactaact accaaacaca gtgatggtcc
    10681 atgcgcaatt ttcggtgacc gggtgcatct gcgtcatgtt ttgtacaatc tgctcgacaa
    10741 tgccaacaag tattcgcccg aagcgccgga aattaccatg ttgtccaata caaacaatga
    10801 gcgtgtacag gttgcaatca gtgatagagg agtagggata agcaaggaag accaaaaacg
    10861 tatttttgaa catttgtacc gcgtgccctc ggggaatata cataatgtga aaggttttgg
    10921 gctgggattg agttatgtga aagccgttgt ggaaaagcac ggcggaacag tttccgtttc
    10981 gagtgatccg ggaaagggaa gtacatttac aattgaaata ccactccgtc atgactaaca
    11041 cgacgaatat cttattggtt gaggatgatc ccaacctcgg aactttgctg aaggaatacc
    11101 tgaatgcaaa aggatttcca acaacgctct gcaccaacgg acaactcggt ttcgaagctt
    11161 tctgcaaagg gaaattcgac tttctgattc tggatgtgat gatgccggta aaagatggct
    11221 ttacgctggc aaaagagatt cgaaagttga atacaaaagt cccaatactt tttctcaccg
    11281 cgaaatcgat gaaggaagat acgcttgaag gattcaatgc aggaggagat gattacatga
    11341 ccaagccctt tagtatggat gaactcatgg cccgaatgtc ggccatactg cgaaggataa
    11401 acggggataa aaaagtacta ccggaaattg tacagatcgg catttattcc tacgacagca
    11461 accgtcagga attaaaaagc ggtgatcaat cccagaaact taccacaaag gaaaatgaat
    11521 tgatgaaatt actcgcaggt aacatcaatg gtttggtcga acgcaatttc gctcttacag
    11581 cagtgtgggg cgacgatagt tattttaacg gacgcagcat ggatgtttac atcaccaaac
    11641 tccgtaaata cctcaatgca gatccgaatg ttgagatcat gaatattcac ggaaagggat
    11701 ttcggttggt ggtgaagtga gttgactacc actaagtcac taagtacacc gaggatcacc
    11761 aagtgattct ttgtgctctt gtgtcttagt ggtggaaaga tcaaacccca taaagtttcc
    11821 ccggaaaaga ttttacaata cccttaaact ccatcgttaa caacaaagct gaaactttac
    11881 tcattggcca gttgctttgc aggcagagag cgtctattcc ggtctgattg ttttcttgga
    11941 gcaaatcgaa tacaattttt tcatccggat caagatcagg gaataaggga atctggatag
    12001 catttttttt actgttgttt tcttcccatc ccatggtgag taaaatatca ttcgcgtttt
    12061 ctaccagtgc agcgcggtga atttttatca gccggttgca tcccgaactt ttttcatctc
    12121 ccggtcttcc cggaaatgca aacacatcgc ggttgtaatc gtttgcgagc attgctgtga
    12181 tgattgaacc gcctttgttt cctgtttcta tgactatcgt tgcatcgctc attcctgcta
    12241 cgatgcgatt tcgtttcgga aaattctcgc ggtcgggatt ggtttcgctt atgaattcgg
    12301 tgagtaatcc accttgttct aacatttttt ctgcagtgct tcggtgtgca cccggataaa
    12361 tgcgatcaag tccgtgagcc aaaacaccca cagttggaat cgcgtgttgc aaggaagaac
    12421 ggtgtgcgca gatatcaatt ccataggcga gcccgcttac cactgtgatg ccttttcctg
    12481 ccaaattttc tacgagtgat gtgcagattt cttttccctt ttccgagggc agacgtgttc
    12541 ctacgatact aactaccctg gatgtgttga gatcggcatt gccaagggag aaaagtacaa
    12601 ggggcgcatc ttcgcaattt tttaagcgtt gcggaaatgc actgtcgaga aaagtgcagg
    12661 ctctgatttt gttgcgacgg atgaattcca tctctttttc agcacgctcg agtacgcctg
    12721 tgtggatggc agacgcaatc acttccccaa ccccgggggt gcgcatgagc tggtggcgtt
    12781 tttgtttaaa gacctgctcc gccgatccgg caaaagcaat tagttttctg gcatttaccg
    12841 gacctatacc ctccaacagc ccaagtccga tggtataaaa taaacgttcg tcggaatcca
    12901 ttagcaacgg atgaattatt tgttatattt cgtgaagtta gacgctacag attgttcatg
    12961 aagacaaaat tcgctgttca taaaagtgca gtattcgtac tgttcgctct ttttatcagc
    13021 cttgtttcca aggctcaaaa cgcaaccatt cgcggtaaga tcacagataa atcaaccaaa
    13081 gaagctctgg ttggtgttaa tgtggtgtta agcaaaggtg gaggcgttgc cagtgatatt
    13141 aatggccaat ataaagtttc ggtcgatcct ggtgaggtga aaatttcttt tcgctttatt
    13201 ggctacaagc aggtggacat gacacttcaa ctcaaagcgg gggaagataa agtgctcgac
    13261 gtcgtactcg aagaagcgaa cactgaactc gaaacagttg tggtttctgc aggacgattc
    13321 gagcaaaaac tggaagatgt taccgtttcg atgaatgtga ttaagccttc acttattgag
    13381 aataaaaaca ccttgaattg cgaaaccatc atcgatcagg tgcctggtgt ttccgtgcag
    13441 gatggtcagg caagtattcg tggaggaagc ggctttgcat atggtgctgg tagcagggta
    13501 ttgctgatgg tggatgatct tcctttgctc gcggcagacg caggtgatgt aaaatggaat
    13561 acactgccca ttgaaaacat tgaacagatc gaagtcatta agggagcaag ttcagtgctt
    13621 tttggttctt ctgctttgaa tggggttata aatatccgaa ctgcctatcc aaccgataaa
    13681 cccgtcacaa aaattgcaat caccagcggt ttgtacgata atccgcgacg cgatactgcg
    13741 cgttggtggg ataaaaatcc tgcttatcac ggaatcaatt tttttcattc ccgcaagatc
    13801 gatcgttttg accttgttgt tggcggcgct gcattttccg acaaaggtta ccgcatggga
    13861 gaagaggaat atcgcggacg gataaatttt aatacacgct acagaagtaa gattgcaggg
    13921 ctgtcgtttg gagtgaacgg caattaccaa tacagcaagg cggggattta catcatctgg
    13981 aaaaatgcaa acgagatcta tgttccttcc ggttatgatc ctgcacttgg ctgggacccc
    14041 tcaaaaaatc ccaactccac catcagtatt aatttcggaa accgattgaa tattgatcct
    14101 tatatcacct attttgcaaa gaacggcgat cgtcacgcat tacgcacaag ggtgtattgg
    14161 acacgtaacg taaacaacac caatcagagt gctgatgcgt acatgactta tggtgaatat
    14221 cagttttcgc gaagaacaaa aagcgatttt aatattactg caggattagt gggttactac
    14281 agcaaggtgc tttctcagtt gtacggaaat catttcggaa caaatgcggg tgcctttgtg
    14341 caggttgata aaaaactcaa ccgttggaca ctctctgcag gaatgcgtgg tgaatatttc
    14401 cgcattgata ctgcagaaac ggtttcgcgt tcttccattg ggaatttaaa cattaacctc
    14461 ccctttaaac cggtgtttcg cgcaggagcg aatttcagag cacgtgaaga aacgtatatc
    14521 cgcgcatctg tcggacaagg ttaccgtttc ccaaccattg ctgagaaata cgtgagcacc
    14581 gctgttggag cacttaatat tttcccaaat ccgaatttat ctgcagaaag tggatggagt
    14641 gcggagctgg gaattaaaca aggcgtaaaa attggaaagt ggctgggtta tgctgatgct
    14701 gcgctttttg tgacgcagta taccaatatg atggagtttg catttggtgt ttataatccg
    14761 gatagtattc cgcttaattt gaatcccaat caccccggct atcttaccaa ctgggttgga
    14821 tttcgggctc agaatgcaga ggaagccaga gtttcgggta tagatttgag tattattggt
    14881 aagggtaaaa tattcaagaa aatagatatg actgtttttg caggatatac ttatatgcgg
    14941 ccgattacac ttaacacaga tacgaattat ctgtattcct ttagcgacgc cgcaacaata
    15001 cgtaccattg acggtaaaga tgccatgatt ctgaaatatc gttttcagca catggcaaag
    15061 gccgatgttc aattcgacta caaaaagata ggttttggtt tcagtgtgcg ttacaacagc
    15121 aatgtgtaca acatcgaccg caccttcgaa aatctggaag taaaaattgg cgggggtcaa
    15181 cttcctctgg gcgattatat tcttcccgga ttgcccgaat accgaaaaga acacaatcgc
    15241 ggcgcatgtg tagctgatat gcgtttggca tatacggttg caaagggaac ccgcatcgca
    15301 gctgtggtta acaacgtctt taaccttgag tatatgggta gaccaggtga tgtgcaggct
    15361 ccccgtactt ttgcgttaca agcagtagtt acgttttaac ttatatgctt tagcctatag
    15421 gttaaatccg gagactaatg tttcacaacc atattgtagc cggggacgtc atttcccggc
    15481 tacatcaaat atatctcgct aagtgcagaa tcaatatccg gataaagaaa tgtaaatccg
    15541 ctttgaatca gtttctctga tgatgcccgg ggtccctcca ataccagttg actcatctcc
    15601 ccaaacgcaa ggcggataaa gaatgcagga acgtttggta aaaatacagg cataccaact
    15661 gcacgaccta tcgctttggt taattcttta ttgctgaggt gttgaggagc aatggcattg
    15721 aagtttccgt tgagtgattt gttttgtata gcgaaaagaa aacaacgcgc agcatcatcg
    15781 gcatgcatcc agggtgtcca ttgtttgcca cttccaatag cggcaccgag accaaactgc
    15841 accggaaaac caatgcgggg aattgcgcct tcgtttttat cgagcaacat tccaaaacga
    15901 aaacacacta cccgacacaa atcgtcaaac tgatgtgcgg ctttttccca ttctatcacc
    15961 acattcgaaa caaagccttc tccgggcttg tcttcttcgt tgtggatatt atcgcttgtt
    16021 gcgttgtaat aattgattcc tgaggcggaa aagaatcctt tgagtggttg attgcgttct
    16081 ttgagtttgc ggaacaacaa cgcaggacca tcaattctac tgcgcagaac ggaatgtttg
    16141 cgagcttcgg tccattttcc atcggcaatt ccttctcccg ctaagtggat gatataatct
    16201 actccatcca gtgcgcctgc ttcaatcgtt cctttacggg tatcccataa ccaaactttt
    16261 acaggattcc tcgaattgcg cgaacgacca agatgcgcaa cctgataacc ttcctttaat
    16321 aatcgttttg taagccgtgt acccagcatt ccggatccgc cggttatgag aattttttcc
    16381 atcacgaaaa ggtaacaatt aaaagaacag tattgttttt cggccatgcc acgtatatca
    16441 tgatccctat catggggaac aagagaaaca tgatgtatct tagactccta agcgcatcaa
    16501 tagtggacaa tctccccaaa tccccttccg atcttcaacc tcattccatg gatcaggaaa
    16561 tcattgctgc taccgcgcaa caagtgatca aggattttgg agagttttca ttcgaaattg
    16621 ttttcaccgg aaaaggaact tcgccctacg ccgaactttt cgaacaggtt ttaccggtcg
    16681 ttgaacattt gcttcagacc aacaccgaac gttttcacgc cttattgtac cgaatagatg
    16741 taaatgaatc cctcatcaaa cgctattccg ccgaaacacc cggtatgcgt tttcctgaaa
    16801 tggtcaccga tctcattctt cgccgcgaaa tgatgaaagt actaacacga agatattttc
    16861 ggtagggttt attttccaat taactaattt ccaattgggt gaattgtacc attctttatt
    16921 tttcgtacaa tatggtcgag aatgaacgat aggcaagtca attgataatt gggttattag
    16981 aaaatcggca attaaccaat gaaatgaacc ttgcccaaat ccgcgtgtac agcctttcaa
    17041 aacctccaaa aagatgaaaa agataattct tttggcgacg ctgttcctga tgggtgcagg
    17101 tgcctctgtt aacgcgcaat acaatgcatg cgcggcaaaa aatgtgatca ccgaaaaagc
    17161 aacggaaacg tatgtggatc aaacttccgg acaaaccgta acaagaacgg tcgaacgcaa
    17221 ggtgaatgcc gacaaacacg gcaatgcaaa tggtaatcag tacgacctcg ctgttgacgg
    17281 tgcattcgac ggacaaacca ttgttgtttt gcatttgtat accggcgaag gatttgattt
    17341 cagtttacct aaagctgcat tgcaggaaaa aggattttct gtttaccgtt atatgaatca
    17401 accgccaagt ccgaaagaat taaaagaagc actcgataaa gcatgtcagc tatgggtgat
    17461 ttcaaccact tcgcaattgc tcaatgaaga acatgcaaaa gtgatcaagg atttttggga
    17521 caaaggtcat ggtgtttatc tctggggcga taacgatcct taccatgccg atgcaaatta
    17581 tttatcgcgt taccttattg gtgcagatat ggaaggattc tattatgcag cgaatgttgt
    17641 tacgctcaaa agcgactcca ctgtttccgg attgcagcgc gatcacctga ttactaccgg
    17701 actcgaatat gtttatgagg gaatcacaat cgctaaaatt catgatcata aaaatgtatt
    17761 gactcctctc atttggagca gtgatggaaa cgtggttacc tccgtttatg aaaaagatgg
    17821 caagcgtctt atacttgatg gaggatttac aagactctac atcaattggg atactgcagg
    17881 taccggtcgt tatgtaaaga atgcagctgc ctggctggtg aattacgaga agtttggtga
    17941 tgctgttgtg agtacgaagg tgaagagcaa atagaagacg gggaagtatg acgaagacag
    18001 tgaggcatta actcctaacc ttaactaatt actgacaggt gagcattgaa ccccgaacaa
    18061 tttaccgcaa aggcgaggtg ttggaagggt ggatgattcc aaattcgtaa tcaccacgat
    18121 gtttgacttt accggacgat ctttttaagt ggagctatga cacc
//
LOCUS       DAOMYG010000009        18016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1073_length_18016_cov_9.474138, whole genome shotgun sequence.
ACCESSION   DAOMYG010000009 DAOMYG010000000
VERSION     DAOMYG010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 18016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18016
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1073_length_18016_cov_9.474138"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="PK610_00705"
     CDS             complement(<1..1124)
                     /locus_tag="PK610_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRJ34472.1"
                     /translation="MDYLVQAGQFLLSLSILIVLHEFGHFIPARLFKTRVEKFYLFFN
                     PWFSLFKFKKGETEWGLGWLPLGGYVKIAGMIDESMDKEQLSKPAEPWEFRSKPAWQR
                     LIIMLGGVTVNFILGYFIYIMVLFVWGREFLPTENASYGMYVRNEVLHGEGLLQHGDI
                     LVGVDGKQPRTYQDAGKMILIDGARQIEVIRNGSTHLVTLPENIVDQLLESGKSSLFE
                     PLFPFVIEKITSGGIGEQAGLKMNDSLVGITRDFKRPVFFPESVFPDSLFGLKRIDAP
                     YFQTLAYQIQRSANQEITLHIYRDGVPMDVNAKLDEKGLLGIQPYRADRSLVFQTETF
                     SFLESIPEGIKTGTETLSNYVKSLALLFSPAGAKQLGGFGS"
     gene            1238..2419
                     /locus_tag="PK610_00710"
     CDS             1238..2419
                     /locus_tag="PK610_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34473.1"
                     /translation="MRLISFYYLTLLSLLPMAANADSPLTSTPFYTAYMDIKAIKDHE
                     SAERISAEGFQFLDGNAPADQKAAFVNALGWGKMQFVNSYREHLGKKYGLPAVFFDSI
                     LVYKGDEPEALAGTEKLTQDELMCLGYMQAMGNYFEPLKGHYCAYLAFNRGVHRSQAM
                     VYGLIVAQYNLDRNWCYVYQVMEYCRDQSGIEPDEFRDEAMTIIFEYINLYQSECEEE
                     PVYEEEETPFGLVQPKPDYYYLYDSIQANKGKKNFADLVIEEIIRPEYFEEYQGTKVK
                     VIIRNTGSSSSFPALAKMSEVDLSLAEAKRIGLKGDVLQYAAELDNRGDDPGADDPYF
                     ESWTYIPTIEPGKRYVIEFVVLGYWIYDPNCEFKVELDTEKNITEPNEKNNVMFFVEG
                     G"
     gene            complement(2416..3579)
                     /locus_tag="PK610_00715"
     CDS             complement(2416..3579)
                     /locus_tag="PK610_00715"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRJ34474.1"
                     /translation="MNQEKPKKGIAVLGSTGSIGTQALQVVAEHSNSFSLEVITANRN
                     ADLLIEQALKFDPNTVVITDVTLYQKVKDALSHTDIKVFTGEQSLCDVVTMSSIDVVL
                     TAMVGYSGLPPTIAAIKAGKDIALANKETLVVAGALITRLSKENQVNIYPVDSEHSAI
                     FQCMAGEFHNPIEKIYLTASGGPFRGKKAEELKSVTKAQALKHPNWEMGAKITIDSAS
                     LMNKGLEVIEAKWLFGLKDEQIDVIVHPQSIIHSIVQFNDGSMKAQMGLPDMRLPIQY
                     ALTYPDRLPSSFERFNFLEYPQLTFEQADRATFRNLDLAFLAMKKGGNTACVLNAANE
                     IAVQAFLEEKIGFLDIARINETCMDKIDFIKDPALEDYISTNLTTRRFAAELV"
     gene            complement(3576..5747)
                     /locus_tag="PK610_00720"
     CDS             complement(3576..5747)
                     /locus_tag="PK610_00720"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ34475.1"
                     /translation="MPAFIRLILLLVIVSSWGTNSAFAKDEKSLREQLQKSQQLALSY
                     PDSAIKIAEQALTQTSTPELLSMAHSIMGVALVHKGDFEPALFHHRKSLALTIKHNID
                     SQLGNTYNNLGVAFDYLGNYDSAVFYYEKGLELRIKSGKLNSVQGSLSNLGLVYYLKG
                     DLNKSLQYLFPSLKIAESTKDNNGMVSSLINIAIVYEKLKKYDESIALLKQAISIAEK
                     MNWRADLALCYNNLGVIEVGRFNYQASLEHHFKALKIREEIGDKGGLAMSYQNIGSAL
                     YHLNQFNDAVGYLDKGLVLSDSISDLITSMEILNVKALASAKTNKKAEALQYVNRAIA
                     IAENHQDYINRYQVYRAASVVYEANGQFKEALGYNKLYHQLLDSVENQENTNMLVEKE
                     QAYKYEKKKQEDEFIANQKRLKQEAEITRQNMFNYLLGLGILSLLIILGFILRSLKQR
                     KKANELLTTRNNLIENQKKLIEEKNKDITDSIQYAKRLQQAILPTQKELNYLKDHFIL
                     FEPKDIVSGDFYLVIPLSENDVIFGVIDCTGHGVPGGFVSIVAHNALMRAVKEFGIKE
                     PAAILNKVNELIDEIFHKEGKDAIQDGMDIALCRLNNRELQFAGANNPIWIFDEQNCI
                     EIKGDKQPVGAYVHRKPFTQHTMRLQQGQLLVLFSDGYADQFGGPGGKKFKYSRMKEL
                     IQTNKQQHPEKIRKVLLENILSWRGTLEQIDDICVLGISIR"
     gene            complement(5803..9315)
                     /locus_tag="PK610_00725"
     CDS             complement(5803..9315)
                     /locus_tag="PK610_00725"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRJ34476.1"
                     /translation="MQKRFFILLALFFNLSINAQVRNFRFYGLDEGLSQSQVSTICQD
                     HNGYIWLGTLGGGLNRFDGIDFVSYSMIDGLSSNMILKVFSDSKNTIWIGTDRGICKY
                     DGKRFEKVPTPFDLSDKAIWDITQDKKGNIWFATGGYGVLRYDGIKFYKYGINDGLGF
                     SHAYRILCDQSGTVWVSSKGYGFARFKGELFENVADISTSVNLNIDDISETTAGELLF
                     FTPQGTFQLIGNKFITAGLSQEVNGEVIASLVDKNGSLWVATNTNGIFRFSKDGASAE
                     HFKSENGIPDVAPLCLFEDKQGNVWIGFDGGGCAVFMGDRFIHFNKQTGLPNQFVRSI
                     LQDNAGKIWFGTENGIAVYDENKFTILNEETGMPNAFVHSLYQDKKGRIWAGTEDGPI
                     LFDGTKINHYPYSSETEVGQVFCFLEDPESGELYIGTENGIMVFNGNEFENRYKKQLP
                     GVLVFSMRLMGRNDIWVSTNKGLFRFNGTNFHQQVLKDEYGPLDAVDCWKDPKGNTWV
                     ITSRGIVILRKNGTKEILRTKNGLHADNLYLMVFDGRDLWIGSDKGLDRIIPDEDWAV
                     KEIRHYGKEEGFTGMECNANSVLKDRKGRLWFGTIKGVTCYFPDKDKLHPTPPPLLLT
                     GMRLLYEKTNWKEKYQDDQFSSEMPDNINLHPGDDHITFDFIAFDFYNPKNIRYRYML
                     EGVDQDWSPPDKENSATYSYIPPGEYRFAVKACNSDGFWSEPVYFSFSVDAPFWKKKW
                     FYALLLPLVVILFYVFLLIRTRNLNKAKRQLEEKVRERTSELNAQKEQLQKLSIVASK
                     MNEGVIICDQNGNIEWYNDSYFKTIGYTKEQYFESAYGKVKTLQELSSYKSLSTVFKN
                     FPKTREAIVYDSPHQNQQGEVEWTRSSIVPVFNDADELNKIIAIYTNITDRKNVEIAL
                     EQANKDFLDSVKYAKRIQEAVLPHAVILENEFPNSFVFYKPRDIVSGDFYWFAKVNNV
                     FLWALADCTGHGVPGAFMSLIGNEYLHQVTHNALFTGPEQCLHFLDKQITRALHQDGG
                     DKDSRDGMDIGLCAIHTKDNLCQFSGANIPLYLIRNGEVIEMEAVKEAIGGYNERGKD
                     FYSHEFPILPGDTMYMTSDGFIDQFGGTNGKKFMRRNFRELILRIHMLPMEEQKTIIE
                     TEFNNWKNDRKQVDDVLVIGIKIP"
     gene            complement(9347..10153)
                     /locus_tag="PK610_00730"
     CDS             complement(9347..10153)
                     /locus_tag="PK610_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRJ34477.1"
                     /translation="MVRLFTFILLMASASTWANKDTTISVQQNNQKRVVHLSFPKVKA
                     KGSILLLHGWNFPATQWCDKTNIGSLALIKGYVLIMPDLGKSTYHWQTYPETIEKYRS
                     FPTRRWITDTLITALQNKFSLLLPGKNNFVAGLSTGGRGAALLALENPKIFKAAACLS
                     ADFDQRLLLNEPINTGFYGSQEKFPERWSGRDNIYLRAKEFNVPLLLIHGKLDKMCPY
                     TQTENFVKELTRTNPALKVVQHIDENGSHTYSYWGSHSETILRFFDEHLK"
     gene            complement(10445..10515)
                     /locus_tag="PK610_00735"
     tRNA            complement(10445..10515)
                     /locus_tag="PK610_00735"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10481..10483),aa:Cys,seq:gca)
     gene            complement(10557..10907)
                     /gene="folB"
                     /locus_tag="PK610_00740"
     CDS             complement(10557..10907)
                     /gene="folB"
                     /locus_tag="PK610_00740"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRJ34478.1"
                     /translation="MAIVSINNLKVYAYHGCLPEEAMVGTNYRIDMDIHIDFEQAAIE
                     DNLDLTADYVVIAQLIKEEMAVRSKLIETVARRIILRTRETYPKAEKIKITIYKINPP
                     AEADLHSVSVSMEG"
     gene            complement(10910..12889)
                     /locus_tag="PK610_00745"
     CDS             complement(10910..12889)
                     /locus_tag="PK610_00745"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ34479.1"
                     /translation="MIRLPILLCVLLLFPQLLKAQSPVDSLLRVVANSDYDTAKVEAY
                     NELIWEYYGANNDSALYFGKLSIDLAERIGYKKGLSNAHRYTGNVYEQMSDYAKALYH
                     QQQGLNIAESILFKPAIASSLSNIGSIYERLNDQKKALDYLERSLKMCIEIDQKEGIA
                     AVNGNIGNIYFNIKAFNKALEFYQRSLEMNQELENPYGIAVSLSNIGNVHIEKGDFKK
                     GLEYHTQSLEIRTRINHRQGMASSMASLGVCYQNLGDVSTAKKYFSDAIQLSNEIHDM
                     LSGVKAYKGMYKIHRELKEFEKALFYHEEFTRLNDSVFSTQRNEEISNMRTKFALEQQ
                     QKELDRKAEEEMIRIQAKADGEKQQQRIIIYASVAVLILVVLFSLFLFNRFRVISRQK
                     GIIETQKAVVEETNKQITDSINYAKRIQTALLPTEEDIKTIFPESFVLFMPKDIVSGD
                     FYWASSPDSDTTIIAIADCTGHGVPGGFMSMLGTSYLNEIVNEKSVYEPAKILDLLRD
                     RIIFALKQQGHAEESKDGMDISICKFEKRNNRVVYAGANNNVYVVRNNTLSEYPANKM
                     PIGYHAGKMIAFSQQEIPLEKGDMVYAFTDGYADQFGGPDGKKFKYAQLEKLLCSISS
                     STSSDQKKKLENAFQEWKLEIEQVDDICLLGIRYI"
     gene            complement(12929..14470)
                     /gene="gltX"
                     /locus_tag="PK610_00750"
     CDS             complement(12929..14470)
                     /gene="gltX"
                     /locus_tag="PK610_00750"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRJ34480.1"
                     /translation="MNNRRVRVRFAPSPTGPLHMGGVRTALYNYLFAKQNGGDFLLRI
                     EDTDQARFVPGAEAYIIESLKWCGICADEGVGFGDGPHSPYRQSERKEKGIYAQYSDQ
                     LIKSGHAYYAFDTPEELEEMRKRLEAAKAKSSSYNAITRQHMKNSLTLPEDEVQKRLA
                     SGNPYVVRIKMPRNEEVKLKDIIRGWVVVNTAELDDKVLFKSDGMPTYHLANVVDDYL
                     MQITHVIRGEEWLPSAPLHILLYRYLGWENVMPEFAHLPLLLKPDGNGKLSKRDGDRL
                     GFPVFPLEWKDPASGEISSGYREKGYFPDAFINVLALLGWHSSGNQEIFSLEELIKEF
                     LLERVSKAGAKFDPVKTLWFNQQYLRKQSPEQLADLWQPELNNKGVKADKAYTTEVCR
                     LLQEKAHFVTELWSLGSYFYQAPESYDAEVIKKRWNEKSEAFIVTITTAFRNSTTFNA
                     ASAEEMFKSACETVGVKTGEVMQLFRVCLSGAAGGPALFEMAALFGKDVCVQRLEKAL
                     AIIKS"
     gene            14657..>18016
                     /locus_tag="PK610_00755"
     CDS             14657..>18016
                     /locus_tag="PK610_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34481.1"
                     /translation="MIDKLDAFIRKYYKNLLIRGSLLSFSTVLIAFLFVILVDQWADL
                     SATGRTILFFGFVGTFLVALSIWVIDPLVRYFRLGKVISHEQAAVIVGEHFSEVGDKI
                     INTLQLGRQAVLTSGQLDLLNASIQQRVIDLRPVPFTSAIDLRKNKKYLRFALPPLFV
                     AVALLFAAPSVLTEGSFRLLNFNSEFEAKTPFSFDLLSDTLEVVEQDDFSLELELNGT
                     QLPAEVKIVAGEENYLMERGDKRHFNYVFRKVARDIRFRFSADGYTSKEYVLKALPNP
                     SLLSFEVQLDFPKYTGIAPKVISNSGDLIIPEGTSAKWIVRTKNTDRLTVRLQDSLLR
                     LNPAPKDQFSFGARLMSSGTYALLTGNKFTTSRDSLTYFLQVIPDQHPVIDLDEFQDS
                     TSLRRKYFTGQISDDYGFTRLAFHYRFLESGDSVQDKSLKSIELPFSRQSAADRFVYF
                     WDLSQLNILPGDQIEYYFTVSDNDGIHGPKSTRSQTRIFRAPSLQEISANNDKNNSEI
                     KDKMEKALIEAKKLSKDMKVTKQELLEKKNPGWQEKKKMEDMLERHQQLQKQLEDIRK
                     QNEQNNQQKTEFKDINESLMEKQKQLEDMFNKLMNDEMKKLYEELQKLMEQMNKEKVQ
                     EQMEKMEMSNEQLEKELDRSLELFRQLEMEQKMQDITDKLKELAEKQDKLSEDTKNKT
                     ESQKELEKRQEELNKEFKDVKEDFKKLQEMNEELEKPKELPETEESQKKIDEEQQKSS
                     DDLQQGKNKKASESQKKASDEMKKLQMQMESAMESMQAKQNEEDMNAIRSLLENLITL
                     SFDQEALMKEFEKTNPKDPRYVKLGQQQRKLKDDAKMIEDSLFALSKRQISIQSVVNQ
                     EMALINENMNSSLENIGERRTPQVRMNQQYVMTSLNNLALMLDQALQQMQQQQKNQGK
                     GGGGGSCNNPNGSGSPKSGKKPGQGQGSGKGQTPQQSLESIKKMQDELTKTLERMKKS
                     LEEQQGQQQGGKKPGGKQGSGLGMPGENGMTSQELAEMAAKQEALRKRLMELSNELNK
                     DGSGSGNGLKKIAEEMERNEEDIVNLNINRQTLHRQQEIMTRLLESEKALRERELDEK
                     REANESKNQEYSNSFQFLEYKRQKEKEVELLR"
BASE COUNT         5096 a         3739 c         3778 g         5403 t
ORIGIN      
        1 gagccaaatc cgcccaactg ttttgcgccg gctggggaaa aaagcagagc aagtgatttt
       61 acgtaattgc ttaatgtttc tgttcctgtt tttatgcctt caggaatgga ctcaagaaag
      121 ctgaacgtct ctgtctgaaa caccagacta cgatctgctc tatagggctg aatacccagc
      181 aacccttttt catcaagttt tgcattcaca tccataggaa cgccatcacg ataaatatgc
      241 aaagtgattt cctgattggc cgatcgctga atctgataag ccagtgtttg gaaataaggg
      301 gcatcaattc tttttaaacc aaacaaacta tccggaaata cggattccgg aaaaaacacc
      361 ggacgcttaa aatcgcgagt gattccaaca aggctatcgt tcattttcaa tcctgcctgt
      421 tcaccaattc ctccactggt aatcttttcg attacaaatg gaaacaacgg ttcaaaaagg
      481 gaagattttc cggattcgag caattgatct actatatttt caggaagtgt taccagatgt
      541 gtgcttccgt tgcggataac ttcaatctga cgtgctccat caatcaaaat catctttcct
      601 gcatcctgat aagtgcgtgg ttgtttcccg tcaactccta ccagaatatc gccgtgttgt
      661 agtagtcctt ctccatgcaa cacctcgttg cggacataca ttccatagct ggcattttca
      721 gttggcagaa actcccgccc ccaaacaaac aataccatga tgtaaataaa gtagcctaga
      781 ataaaattaa ccgtaacacc accaagcata atgatcaatc gttgccatgc cggtttgctt
      841 ctgaattccc agggttccgc aggtttcgaa agctgttcct tgtccatgct ctcatcgatc
      901 atcccggcaa tttttacata gccaccgagc ggtaaccagc ccaaacccca ttcggtttcg
      961 ccttttttga atttgaaaag tgaaaaccag gggttgaaga acaggtagaa tttttctacc
     1021 cgggttttga acaatcgcgc aggaatgaaa tgtccaaatt catgtaaaac aatgagaatg
     1081 gaaaggctga gtaagaattg tccggcctgg actaggtaat ccatggatga atgattttgc
     1141 caaaaataca attttatcaa cccgcatcac tgaaatacgg taaaggcctt ataactttcg
     1201 cccggtaata gcgtactgta atcaaaacac ccgttcaatg cgattaattt cattttatta
     1261 tctgactctt ctctctttgt tgcctatggc agctaatgca gattctcctc ttacatccac
     1321 accgttttat acggcgtata tggatattaa agccattaag gaccatgaat cagcagaacg
     1381 aatcagcgcg gaaggatttc agtttctgga tggcaatgct cctgctgatc agaaggcggc
     1441 atttgttaat gctcttggtt ggggaaaaat gcagtttgta aacagttaca gagagcatct
     1501 cggtaaaaag tacggattgc cagctgtgtt tttcgactct attctagtgt ataaaggcga
     1561 tgaaccggag gcactcgcag gtacggaaaa attgacgcag gatgaattga tgtgtctcgg
     1621 gtatatgcag gcaatgggta attattttga accgcttaaa ggacattatt gcgcctacct
     1681 tgcgtttaac cgaggtgttc accgcagtca ggcgatggtt tatggattaa tcgttgccca
     1741 atacaacctc gatcgtaact ggtgttatgt gtaccaggta atggagtatt gcagagatca
     1801 atcaggtatt gaacccgatg aatttcgtga tgaagcaatg acaattattt ttgagtatat
     1861 caacctgtat cagtcagaat gcgaagaaga accggtctac gaagaagaag aaaccccttt
     1921 cggtcttgta caacctaagc ccgattatta ttatttgtac gattcgattc aggccaacaa
     1981 gggaaagaaa aattttgctg atctcgttat tgaagaaatt atccgtcctg aatatttcga
     2041 ggagtatcag ggaacaaaag tgaaagtgat tattcgcaat acgggcagtt caagtagttt
     2101 tcctgctctt gcaaaaatga gtgaagtgga tttaagtctt gccgaggcca agcggattgg
     2161 tctgaaaggt gatgttttgc aatatgctgc agagctcgat aacagaggag atgatcctgg
     2221 tgcagatgat ccttattttg aatcatggac ttacattccc acaattgaac ccggcaaacg
     2281 gtacgtgatt gagtttgtgg tgctcggtta ttggatttat gatcccaatt gcgaattcaa
     2341 ggtggaatta gataccgaaa agaacatcac cgaaccaaat gagaagaaca acgtgatgtt
     2401 ttttgttgag ggcggttaaa ccagttctgc tgcgaatcgc cgcgttgtaa gattggttga
     2461 aatataatct tccagagcag ggtcttttat aaaatcaatt ttatccatgc aggtttcatt
     2521 gatgcgggca atgtcgagga aaccgatttt ttcttcaaga aatgcctgaa cagcgatttc
     2581 gttcgctgca ttaagcacac aagcggtgtt gccccctttt ttcatggcaa gaaatgcaag
     2641 atccagattt ctgaacgttg ctctgtcagc ctgttcaaag gttagctgag gatattccaa
     2701 aaagttaaat cgttcaaacg agctgggcag ccgatcaggg taagtgagtg catattgaat
     2761 aggtaatcgc atatccggta aacccatttg cgctttcatg gagccatcat tgaactgaac
     2821 aatgctgtga ataatagatt gtggatgtac aattacgtcg atctgttcgt ccttcaatcc
     2881 aaataaccat tttgcttcta tcacctcgag gcctttgttc atcaatgaag cggaatcaat
     2941 tgtgattttt gctcccattt cccaattggg atgtttcagc gcctgtgctt tggttacgct
     3001 tttaagctct tctgctttct ttccacggaa tggtccaccg gaggcggtaa gatagatctt
     3061 ttcaatgggg ttgtggaatt caccggccat gcactgaaag atggcagagt gctcagagtc
     3121 gacaggatag atgttgacct gattttcttt gcttaaacgg gtaattagcg cgcccgcaac
     3181 aactaatgtt tctttattgg caagtgcaat gtcttttccc gccttgatgg ctgctattgt
     3241 tgggggtaat cctgaataac cgaccatggc tgttaaaacc acatctatgc tcgacattgt
     3301 aaccacatca caaagcgatt gctcgccggt aaaaactttg atatcggtat gtgataaggc
     3361 gtctttcact ttttgataaa gtgttacatc ggtaatgacg acggtattgg gatcaaattt
     3421 taaagcctgt tctatcagca ggtctgcatt tctgttggct gtaattactt caagcgagaa
     3481 actgttggag tgttcagcga caacttgtaa cgcttgtgtg ccaatggatc ctgttgatcc
     3541 aagaactgca attccctttt ttggtttttc ctgattcatc gtatgctaat acctaaaaca
     3601 caaatgtcgt cgatttgttc cagcgtgcct ctccaggaaa gaatattttc cagcaatact
     3661 ttcctgattt tttcaggatg ttgttgcttg tttgtctgaa taagttcttt cattctggaa
     3721 tatttgaact tcttaccgcc gggaccacca aactgatctg cataaccgtc gctgaacaaa
     3781 acgagtagtt gtccttgttg taaacgcatg gtgtgttgtg taaatggctt gcggtggaca
     3841 tatgccccaa ctggctgttt atcgcctttt atctctatgc aattttgttc gtcaaagatc
     3901 cagatggggt tgtttgctcc tgcaaattgt aattcacgat tgtttaaacg acatagtgct
     3961 atatccattc cgtcctgaat ggcgtcttta ccttctttgt gaaagatttc atcaatcaac
     4021 tcgtttactt tatttagaat cgctgcgggt tcttttatcc cgaattcttt taccgcacgc
     4081 atcaatgcat tatgtgcaac aatactcaca aatcctccgg gtacaccatg accggtacaa
     4141 tcaattactc caaaaatcac atcattttct gaaagaggaa taaccaggta gaagtcaccg
     4201 ctgacaatgt cttttggttc gaagaggata aaatgatcct ttaagtagtt taattctttt
     4261 tgcgtcggta aaatagcctg ttgcaatcgt tttgcatact gaatactgtc ggtaatatct
     4321 ttgttttttt cttcaattaa tttcttctgg ttttcgatga ggttgtttct ggtggttagc
     4381 aattcattgg cctttttgcg ttgttttagg gagcgaagaa taaagccaag aataataagc
     4441 aatgacagta ttccaagtcc aagtaggtaa ttgaacatat tttgcctggt gatctccgct
     4501 tcttgcttta gtcgcttctg gttggcaatg aactcatctt cctgtttttt cttctcgtat
     4561 ttgtaggcct gttctttttc tacaagcatg tttgtgtttt cctggttttc aacactgtcc
     4621 agcaattgat gatagagctt attgtatccc aaggcttcct taaattgtcc gtttgcttcg
     4681 tataccacag aagcagctct atatacctgg tagcgattga tataatcctg gtgattttct
     4741 gcaatggcga ttgcccgatt tacatattgt agcgcctctg cttttttgtt tgtttttgca
     4801 gaggcaagcg ccttaacatt cagaatttcc attgaagtaa tcaggtcgga aatggaatca
     4861 ctcagaacca atcctttatc aagatatcca actgcgtcgt taaattgatt aaggtgataa
     4921 agtgcagatc cgatattttg gtaggacatt gcaagtcctc ctttgtcgcc aatttcttct
     4981 cgaattttta aggctttaaa gtgatgttcg agcgatgcct gataattgaa acgacccact
     5041 tctatcaccc ccaggttgtt ataacaaagc gctaaatctg ccctccaatt cattttttca
     5101 gcaatggaga tggcttgttt taacaatgca atcgattcat catatttttt aagtttttca
     5161 tatacaattg caatgttaat tagcgacgac accattccat tattgtcttt ggtcgattcg
     5221 gcaattttca aagatggaaa caggtattgc aaggatttat ttaaatcacc tttgaggtaa
     5281 tacacaagtc caagattgct cagtgatccc tgaacggaat ttagtttgcc tgatttgatt
     5341 cttagctcta aacctttttc gtagtaaaat actgcactgt cataattacc caggtaatca
     5401 aatgccaccc ccaaattgtt ataagtgttg ccaagttgtg agtcgatatt gtgttttatc
     5461 gttagcgcca atgatttgcg atgatggaaa agcgccggct caaaatcacc tttatgtacc
     5521 aaggccacac ccataatgct atgtgccatt gacaataatt caggggttga tgtttgtgtt
     5581 aatgcctgtt cggctatttt aattgcactg tccggatagc ttaatgcgag ttgttggctt
     5641 ttttgaagtt gttcccgtaa acttttttca tcttttgcaa aagcagaatt ggttccccaa
     5701 gaggacacaa taaccaaaag cagaatcaaa cggataaatg caggcattgt gctaaaatag
     5761 agaatatcgg gaagtaatat agagaagaag tgaattattc tgtcagggaa tttttattcc
     5821 gataacaagt acatcatcca cttgtttgcg atcgtttttc cagttattga attctgtttc
     5881 aataatcgtt ttttgttctt ccattgggag catatgtata cgcaggataa gctctctgaa
     5941 atttctgcgc atgaattttt tcccatttgt gcctccaaac tgatcgataa atccatcaga
     6001 tgtcatgtac atggtatcgc ccggtagaat cggaaactca tgagagtaaa aatccttgcc
     6061 tctctcgttg tatcctccaa tcgcttcttt cacagcttcc atctctatta cttctccgtt
     6121 tcggattaaa tagagcggaa tgttagcacc ggagaattga cataaattat ccttggtgtg
     6181 aatagcacac aaaccgatgt ccattccatc gcgtgagtct ttgtctccgc catcctgatg
     6241 cagtgctctg gtaatttgct tgtccagaaa gtgtaaacat tgttccggtc cggtaaataa
     6301 tgcgttgtgg gtaacctggt gcaggtattc atttccaata agactcataa acgcacccgg
     6361 aacaccatgc cctgtacaat cggccagtgc ccataaaaag acattgttca ctttagcaaa
     6421 ccaatagaaa tcaccgctta caatgtcgcg tggtttatag aatacaaatg agttcggaaa
     6481 ttcgttttca agaattaccg catggggcaa taccgcctcc tgaattcgct ttgcatattt
     6541 aacactgtca agaaaatctt tattggcttg ctcaagggca atctcaacgt tctttctgtc
     6601 ggtaatgttg gtatatatcg caataatctt gttgagctca tcagcgtcgt taaaaacagg
     6661 aacaattgag cttctggtcc actccacttc tccctgttgg ttctggtgcg gggaatcata
     6721 tacaatggct tctcttgttt tagggaagtt tttaaataca gttgatagcg atttgtagga
     6781 actcagttcc tgcaatgttt taacttttcc gtaggcagat tcaaagtact gttcctttgt
     6841 atatcctatg gttttaaaat agctgtcgtt gtaccactct atgtttccgt tctgatcaca
     6901 gataatcacc ccttcattca ttttggaggc tacaatagat agtttttgaa gctgttcttt
     6961 ttgtgcgttc agttcggagg tgcgctctct tactttttcc tcaagttgtc gttttgcctt
     7021 gttgaggttt ctggttcgga tcaataggaa tacgtaaaac agaattacaa ccagtggaag
     7081 caataatgca taaaaccatt tctttttcca aaagggtgcg tcaacactaa aactgaaata
     7141 aacgggctcg ctccaaaacc catcactgtt acatgcttta acggcaaaac gatattctcc
     7201 gggtggaata taggaatagg tggcgctgtt ttctttgtca ggtggcgacc agtcctgatc
     7261 aacaccttcc agcatgtacc gatagcgaat gtttttcgga ttgtaaaaat caaacgcaat
     7321 aaaatcaaaa gtgatatgat cgtcgccggg atggagattg atgttgtcag gcatttcaga
     7381 actgaattga tcgtcctgat acttttcttt ccagttcgtt ttctcataga gcagacgcat
     7441 acccgtaagc aacaaaggtg gaggagttgg atgcaacttg tctttatccg gaaaataaca
     7501 cgtaacacct tttatcgttc caaaccataa tcgtcctttg cgatctttta atacggaatt
     7561 tgcattgcat tccattccgg taaagccctc ttcttttcca tagtgacgga tttccttaac
     7621 agcccagtct tcgtcgggaa tgatgcggtc aagcccttta tctgatccaa tccaaagatc
     7681 tcttccatca aacaccatca ggtatagatt gtcggcatgt agtccattct tggttctgag
     7741 aatttctttg gtgccgttct tacgcaaaat aactatgccg cgacttgtga ttacccaggt
     7801 gttgcctttc gggtctttcc agcaatctac agcgtcaagc ggaccgtatt catcttttaa
     7861 aacctgctga tgaaagttgg tcccgttgaa tcgaaacaaa cctttgttgg tacttaccca
     7921 gatatcgttt ctacccatta aacgcatact gaatacaagc acgccgggaa gttgtttctt
     7981 gtaacggttt tcaaattcgt tcccgttaaa gaccatgatc ccgttttctg ttccaatata
     8041 tagttcgccc gactccggat cttcaagaaa acaaaatact tgcccaactt ctgtttcgga
     8101 tgaataggga taatgattga tttttgttcc gtcaaacaaa ataggcccat cttctgttcc
     8161 cgcccagatt cttccttttt tatcttgata aagcgaatgc acaaaagcat tgggcattcc
     8221 cgtttcttcg tttagaatgg taaatttatt ttcatcgtaa acagcaattc cgttttctgt
     8281 tccgaaccag atctttccgg cattgtcctg taaaatcgat cgtacaaatt gattgggtaa
     8341 tcctgtttgt ttgttgaagt gaataaagcg atcgcccata aaaacggcac atccaccacc
     8401 atcaaatcca atccacacat taccttgttt gtcttcgaat aaacacaatg gagcaacatc
     8461 aggaataccg ttttcagatt taaaatgttc tgcgcttgct ccgtctttac taaagcggaa
     8521 aatgccattg gtatttgttg caacccacag actgccgttt ttatctacaa gcgatgcaat
     8581 gacctctccg tttacttcct gcgataaacc ggcggtaata aatttgtttc caataagttg
     8641 aaaggttccc tgtggggtga aaaacaataa ttcacccgct gttgtttcgg aaatatcatc
     8701 gatgttaaga ttgacagatg tagatatgtc ggcaacgttt tcaaacaact cgcctttgaa
     8761 gcgggcaaaa ccataaccct ttgaactcac ccaaacagtt ccggattgat cacataaaat
     8821 gcgataggcg tgactaaacc caagaccatc attaataccg tatttgtaaa attttatacc
     8881 gtcataacgc aatacaccgt agcctcctgt tgcgaaccag atgtttcctt ttttatcctg
     8941 cgtaatatcc catatggctt tgtcgctcag atcaaatgga gtgggtactt tctcaaatct
     9001 ttttccgtcg tatttgcaaa ttccacgatc cgttccaatc cagatggtgt tctttgaatc
     9061 tgaaaaaact ttcagaatca tatttgaact taatccatca atcatggaat atgacacaaa
     9121 atctattccg tcgaaacgat taagacctcc accaagcgtt cccagccaga tgtaaccgtt
     9181 atggtcctga cagatggttg atacctgtga ttgcgacagg ccttcgtcta aaccgtaaaa
     9241 acggaaattc ctaacctgtg catttatgct gaggttaaag aataaagcaa gcagtataaa
     9301 aaagcgtttt tgcactcctt gtgaagataa caaaaagcag attgagttac ttaaggtgtt
     9361 cgtcgaaaaa acgaagaatt gtttcgctgt gtgaacccca ataggaatag gtgtgactac
     9421 cgttttcgtc gatgtgctga accactttca aggcagggtt tgttcgggtt aactctttta
     9481 caaaattctc tgtttgagtg tacggacaca tcttatctag ttttccgtga atgagtagca
     9541 atggcacatt aaattcttta gcacgcaggt agatattgtc tctgcccgac caccgctcgg
     9601 gaaatttttc ctgggagcca taaaacccgg tgtttattgg ttcgttgagg agtaatcgtt
     9661 ggtcgaaatc cgccgacaga catgcggccg ccttaaagat ttttgggttt tccagcgcca
     9721 ataacgcagc tccacgtcct cccgtagaaa gtccggcaac aaaattgttt tttcccggca
     9781 acaacaagga gaatttattt tgaagtgcgg ttatgagcgt atccgtaatc caccttcttg
     9841 ttggaaaaga ccggtacttt tcaatggttt caggataggt ttgccaatga taggtgcttt
     9901 taccgagatc cggcattatc aaaacatatc cttttatgag agccagtgag ccgatgtttg
     9961 ttttgtcgca ccactgtgtt gcaggaaagt tccagccgtg taatagtaaa atagaacctt
    10021 ttgctttgac tttgggaaac gataggtgca ctactctttt ctgattgttc tgctgaacag
    10081 agattgtagt atccttattc gcccaggttg aagcagaggc catcaaaaga ataaaggtga
    10141 ataagcgaac cataatcaaa aatacaagtt taagaatagc aaaggctatg cccgaaactt
    10201 tgtttggctc tcggtctgat gcaacaaaaa aaccccaaac aaaagtttgg ggcttggttt
    10261 ttattgaggt cccgagcgga ttaaccacgt tgttccgcac tgaacaagct aagtcagaaa
    10321 accacccact cgctctcgct cgctattttt tgttgcacca tccctttcgc tcaaaccgaa
    10381 gtttggctct cgctttggtg caacaaaaaa ccccaaacaa aagtttgggg cttggttttt
    10441 attgaggtcc cgagcggatt cgaaccgctg taggagcttt tgcagagctc tgcctaacca
    10501 ctcggctaca ggacctttat gaggtgcaaa tatacaaaaa tttgaagaac tacattttat
    10561 ccttccatgc ttacacttac actatggagg tcggcttctg caggggggtt aattttgtag
    10621 atggtgattt ttattttttc tgccttggga taggtttccc tggtgcgtaa aataatcctt
    10681 cttgcaacgg tttctatgag tttactgcga accgccattt cttcttttat caattgtgca
    10741 attacaacat aatccgctgt aagatccaag ttgtcttcta tggccgcttg ctcaaaatcg
    10801 atatgaatat ccatgtcgat tctgtaattg gtaccaacca ttgcttcttc ggggagacag
    10861 ccatgatagg cataaacttt aaggttgttg atggagacga tggccatgat cagatataac
    10921 gaattcccaa taagcaaata tcatcgactt gctcaatttc caatttccat tcctgaaaag
    10981 cattttctaa ttttttcttt tgatcagagc ttgtactgga agatattgag caaagaagct
    11041 tttcaagctg tgcgtatttg aatttttttc cgtcgggacc accaaactgg tcggcatagc
    11101 catcggtaaa tgcgtatacc atatctcctt tttcaagcgg aatttcctgt tgggagaacg
    11161 caatcatctt acctgcatgg tagcctattg gcattttatt ggccggatat tctgaaaggg
    11221 tgttatttct gacaacataa acattgttgt tggcgccggc ataaacaaca cggttattcc
    11281 ttttttcaaa cttgcaaatg gaaatgtcca ttccgtcttt gctttcttcg gcatgacctt
    11341 gttgtttaag cgcgaaaata atacggtcac gcaaaaggtc tagaattttt gccggctcgt
    11401 ataccgattt ttcatttaca atttcgttca gatagcttgt cccgagcata ctcatgaaac
    11461 cacccggaac accatgcccg gtgcagtctg ctattgcaat aatagtagtg tcagaatcag
    11521 gtgaactggc ccaatagaaa tctccgctta cgatatcctt cggcataaaa agcacaaacg
    11581 attccggaaa aatagtttta atgtcttctt ccgttggcaa caatgcggtt tgaattcttt
    11641 tagcgtaatt gatagagtcg gtaatctgtt tgtttgtttc ttctacaacg gctttctgtg
    11701 tttcgattat tcctttttgc cttgaaatca cacggaaacg attaaacaaa aacaaggaga
    11761 acaatacaac cagaatcaat actgctactg atgcataaat aatgattcgc tgctgctgct
    11821 tttcaccgtc ggccttggct tgaatgcgaa tcatttcttc ttctgccttt cgatcaagtt
    11881 ctttttgttg ctgctcaagc gcaaattttg tgcgcatatt actaatctcc tcattgcgct
    11941 gtgtgctgaa aacggaatcg tttaagcgtg taaattcttc gtggtaaaag agtgcctttt
    12001 caaattcttt tagctcgcgg tgtatcttgt acattccctt atacgctttt accccgctga
    12061 gcatatcgtg gatctcgttg gaaagctgta tagcatcgga aaagtatttt tttgcagtgc
    12121 tcacatcgcc aagattctgg tagcaaacac caagacttgc cattgagctt gccatgccct
    12181 gtcggtgatt gattcgcgtt ctgatctcca acgattgtgt gtggtattcc aatccttttt
    12241 tgaaatcgcc cttttcaata tgtacattac caatattgct taatgaaacc gcaattccat
    12301 atgggttttc cagctcctga ttcatctcca ggctacgctg atagaactca agagctttgt
    12361 taaatgcttt aatgttgaaa tagatatttc caatgttccc attaaccgca gcaattcctt
    12421 ctttctgatc gatctcaata cacatcttca gacttctttc cagatagtcc agtgcttttt
    12481 tctgatcgtt taatcgttca taaatagaac caatgttact gagcgaactt gcaattgcag
    12541 gtttgaaaag aatgctttcc gcaatgttga gtccttgttg ctgatggtag agtgccttgg
    12601 cataatcact catttgctcg tatacattac cggtgtaacg atgcgcattc gataatcctt
    12661 ttttgtaacc gattcgttct gccagatcaa tactaagttt tccaaaataa agtgcagagt
    12721 cattgtttgc accgtaatat tcccagatta attcattgta ggcctcaacc tttgccgtgt
    12781 cataatcaga attggcaact accctcaata ggctgtcaac cggcgattgt gcttttaaca
    12841 attgcggaaa caacagcagg acgcataaca gtatgggcag acgaatcatc tctctgcaag
    12901 atagtgaata gcggcgaatg gaaaaacttt acgacttgat aatggcaagt gctttttcaa
    12961 gtcgctgaac gcatacatcc tttccaaaca acgctgccat ttcgaacagt gcaggacctc
    13021 cggcagcacc actcaggcaa acgcggaaca gctgcatcac ttctcctgtt tttacaccta
    13081 ccgtttcaca ggcagattta aacatctctt ctgcgctggc tgcgttaaaa gttgttgagt
    13141 tgcgaaaggc agtagtgata gttacaatga aagcctccga tttttcattc cagcgttttt
    13201 tgatcacttc agcatcgtag ctttccggtg cttgatagaa ataactaccc agcgaccaca
    13261 gttcggtcac aaaatgagct ttttcctgaa gcaaacgaca aacttctgtg gtatatgctt
    13321 tgtcggcttt tacaccttta ttgttaagct cgggttgcca aagatctgca agttgttccg
    13381 gactttgttt gcgtaaatat tgctggttga accacagtgt ttttacgggg tcaaacttcg
    13441 ctccggcttt actgactctt tcaagcaaaa attcttttat caattcttca agcgagaaaa
    13501 tctcctggtt tccgctgcta tgccacccca ataatgcaag cacgttaata aatgcatcag
    13561 gaaagtatcc cttttcgcgg tagcctgaag aaatttctcc tgaagcagga tctttccatt
    13621 ccaatggaaa aacaggaaag cccaaacggt cgccgtcgcg cttgcttaat tttccatttc
    13681 cgtctggttt cagcagaagt ggtaagtgtg caaattcagg catcacgttt tcccagccca
    13741 ggtaacgata caataaaatg tgtaaaggag cagaaggaag ccactcctct ccgcgaataa
    13801 catgggtgat ctgcatcagg taatcgtcca ctacattcgc tagatgatag gttggcatgc
    13861 catctgattt gaaaaggact ttatcatcta gttctgcagt gttaaccaca acccaacctc
    13921 taatgatatc ttttagtttc acctcttcat tacgaggcat tttaatacgc accacataag
    13981 ggtttcctga tgcaagcctt ttttgaactt cgtcttcagg aagtgtcagc gaatttttca
    14041 tatgctgacg cgtaatggcg ttgtaagagg agcttttagc tttggcggcc tccagtcttt
    14101 ttctcatttc ctctaactct tccggtgtgt caaatgcata ataagcgtgt ccgcttttta
    14161 tcagttgatc ggagtattgt gcgtaaatgc ccttttcttt acgctcactt tggcggtatg
    14221 gcgaatgtgg gccatcacca aaaccaacac cctcgtcagc acaaataccg caccatttta
    14281 acgattcgat gatataagcc tctgcacccg gaacaaaacg agcctgatcc gtgtcttcga
    14341 tacgaagtag aaaatcacca ccattttgtt tggcgaacag gtagttatat aaagcagtgc
    14401 gcacgccgcc catgtgtagg ggacctgtag gactaggagc gaaacgaacg cggacacgac
    14461 gattgttcat ggggcatgta ttgatatagg gcgacaaaga tagaagagta ctgtcaacag
    14521 aggagcagga attaaaaaaa tgaacccttt ttctgccaag gtgtataatt ggagaaggag
    14581 tcaaaattcc cgatatttac cgtaacataa gaaggtttaa accgtttatc tgtaattgca
    14641 agacaatctt tccatattga ttgataagct ggatgcattc atccggaagt attataagaa
    14701 ccttcttatc cgaggctctc tgcttagttt ttctactgtt ctgattgctt ttctgtttgt
    14761 tattcttgtt gatcaatggg cagacctttc tgctaccggg cgtaccattc tctttttcgg
    14821 gtttgtagga acatttcttg ttgcactttc gatatgggta atcgatcccc tggttaggta
    14881 tttcagatta ggaaaagtaa tcagccatga acaggcagcg gtgattgttg gagagcactt
    14941 ttctgaggtc ggagataaaa ttatcaatac acttcagtta ggcaggcaag ctgttcttac
    15001 cagcgggcaa ttggatctgt taaatgcgag tatacagcaa cgcgttattg atttgcgtcc
    15061 tgtacctttt acttccgcaa ttgatctgcg taaaaacaaa aagtatttgc gttttgcatt
    15121 accgcctttg tttgttgcgg tggcattgct gtttgctgcg ccaagtgtat taacggaagg
    15181 aagctttcgg ttgttgaatt tcaacagcga atttgaagca aaaacccctt tttctttcga
    15241 tctcttgagc gataccctcg aagtagtaga gcaggatgat ttttcgcttg aactggagtt
    15301 aaacggaacg caattgcctg cggaggttaa aattgttgca ggtgaggaaa actacttaat
    15361 ggagcggggt gataagagac attttaacta tgtattccga aaagtagctc gtgatattcg
    15421 gtttcggttt tctgctgatg gatatacgtc caaagaatat gtattgaaag ctcttcccaa
    15481 tccatctcta ttgtcgtttg aggtgcagct ggattttcct aaatacacag ggattgcccc
    15541 taaagtaatt tctaattcag gtgatcttat tattcccgaa ggtacatctg ccaaatggat
    15601 tgttcgcact aaaaacacag atcggctaac tgttcgtctg caggatagtt tattgcgttt
    15661 gaatcccgct ccaaaagatc aattttcatt cggagcacgt ttgatgagta gtggcaccta
    15721 tgcgcttctt acagggaata aattcaccac ctcgcgcgat tcgctgactt atttccttca
    15781 ggtaattcct gatcagcatc cggtcattga tttggacgaa tttcaggatt caacttcgct
    15841 gcgcagaaaa tattttaccg gtcaaatcag tgatgattat gggttcacgc gcttagcgtt
    15901 tcattaccgc tttctggaga gcggcgattc tgttcaggat aagtcgttaa agtcaattga
    15961 gcttccgttc agtcgtcaat ccgctgcaga tcgttttgtt tatttctggg atctttctca
    16021 actcaatatt cttcccggag atcagatcga atattatttt accgtctctg ataatgatgg
    16081 cattcatggc cctaaaagca ccaggtcaca aacgcggatt ttccgtgccc catccttgca
    16141 ggagatatct gcaaataacg acaagaacaa ctcagagatc aaggacaaaa tggaaaaagc
    16201 tttgatcgaa gccaagaagc ttagcaagga tatgaaagtc actaagcagg aattgttgga
    16261 gaagaaaaat ccgggctggc aggaaaaaaa gaagatggaa gacatgctcg agcgccacca
    16321 gcagttgcag aaacaactcg aggatattcg caaacaaaac gaacaaaaca accagcagaa
    16381 aaccgagttc aaggatatca atgaatcctt gatggaaaaa caaaagcaat tggaggatat
    16441 gttcaataag cttatgaacg atgagatgaa aaagctttat gaagagctgc aaaagctcat
    16501 ggagcaaatg aacaaggaga aggtgcagga gcagatggaa aaaatggaga tgtccaatga
    16561 gcaactcgaa aaggaattgg atcgttcgct tgagcttttc cgtcaattgg agatggaaca
    16621 aaaaatgcag gacatcaccg ataagctgaa ggagttggca gagaagcagg ataaattatc
    16681 ggaagacaca aagaataaaa cggaatctca aaaggaattg gagaagcgcc aggaagagtt
    16741 gaataaggag tttaaggatg taaaggagga cttcaagaag cttcaggaaa tgaatgaaga
    16801 attggagaaa ccaaaggaac tcccggaaac agaagagagt cagaaaaaaa ttgatgagga
    16861 gcagcaaaag agttcggatg atttgcaaca aggcaagaat aaaaaggcat cagaatcgca
    16921 aaaaaaggca tcagatgaaa tgaagaaact ccaaatgcaa atggagtcag caatggaaag
    16981 tatgcaggct aaacagaatg aagaagacat gaatgcaatt cgttcgttgc tggagaacct
    17041 tattacactt tcgttcgatc aggaagcctt gatgaaggag tttgaaaaga ccaatccaaa
    17101 ggaccctcgt tatgtaaaat taggtcagca acaacgcaag ttaaaggatg atgctaaaat
    17161 gattgaagac tccttgtttg cattgagcaa aagacaaatc agcattcaat cagtagtcaa
    17221 tcaggaaatg gcgttgatca atgagaatat gaattcttca ttggaaaaca ttggtgaacg
    17281 acgtactcct caagttagga tgaatcagca atacgtgatg acgtccttga acaaccttgc
    17341 attaatgttg gaccaggcgc ttcagcaaat gcagcaacaa cagaaaaatc aaggtaaagg
    17401 cggtggcggt ggatcgtgca ataatcctaa tggtagcggt agtccaaaat caggtaaaaa
    17461 accgggtcag ggacaaggat ccggaaaggg acaaacacca caacaatcat tggagtcgat
    17521 taaaaagatg caggatgagc ttacaaaaac ccttgaacga atgaagaagt cgctcgagga
    17581 acaacaaggc cagcaacagg gcggaaagaa acccggtggt aagcagggct caggtttggg
    17641 tatgcccggt gaaaacggaa tgaccagtca ggaacttgca gaaatggctg caaaacagga
    17701 ggcgcttcgg aagcgtttaa tggagctgag caatgaatta aataaggatg gttcgggttc
    17761 aggaaacgga ttaaagaaga ttgcagaaga aatggaaagg aacgaagaag acatcgttaa
    17821 cctgaacatt aacagacaaa cattacaccg tcagcaagaa attatgacga gacttttgga
    17881 gtccgagaaa gcattgagag agcgggagtt ggatgaaaaa agggaagcaa atgaatcaaa
    17941 aaaccaggaa tacagtaact cttttcaatt tttggagtat aaaaggcaaa aagagaagga
    18001 agttgagctt ttgcgc
//
LOCUS       DAOMYG010000010        17834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1089_length_17834_cov_9.406941, whole genome shotgun sequence.
ACCESSION   DAOMYG010000010 DAOMYG010000000
VERSION     DAOMYG010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 17834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17834
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1089_length_17834_cov_9.406941"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1814)
                     /locus_tag="PK610_00760"
     CDS             complement(54..1814)
                     /locus_tag="PK610_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ34482.1"
                     /translation="MIKKLLPVVGLSVILASCGGGKKGDELKMVEAKGGVVYGGIFKI
                     NEVEDFKNLFPLSINDISSHHIASQMYQGLLKFDQQSLEVVPCIAQSFDVNEDATLFT
                     FTIRKGVKFHEDECFGGAAREVTANDFKYCFDRLCTASGDNKVYWLFKDKVKGANEHY
                     AATSGGKASPAGGVAGIKVIDDYTLSIELNFSFSAFPKIIAHSGCWVYPREAYEKYKD
                     DMRTKVVGTGPFVIKSLRDGQMILLEKNENYWEQDEHGNQLPYLQGVKFTFHKEKKTE
                     LMEFRKKNLDMVWKLPVEEISSVLGSMDEARQGGNVEFELQQVEALSIQFYAFLSTSK
                     VFEDKRVRQAFNHAIDREKLVTYTLQGEGTPASFGIVPPSLPNYPSGNIKGFNFDPDK
                     ARRLMSEAGFPNGRGFPVITLQLNSGGTTNELLAEAIQSMLKENLGVTVKLDVMPLNQ
                     LIEEFESGKADFWRSAWVADYPDPENYLNLFYGKNVPENPSDKSYINFTRYRSDAFDE
                     MFSKALRTTDEKERMELFAKCDQLIVDDAVVMPLYYDNYIRLVQTNVRNFPINAMEYR
                     DLSRVYFAIEEKGKKKKKKK"
     gene            complement(1908..2678)
                     /locus_tag="PK610_00765"
     CDS             complement(1908..2678)
                     /locus_tag="PK610_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009421153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRJ34483.1"
                     /translation="MKTRIITYNINGLRSAISKGWIEWLKSVNPDIVCLQEIKATPEQ
                     IDIRPFEELGYFTHWHPAEKKGYSGVAIFSKKEPDNVEIGCGMPKYDREGRILRCDFG
                     DLSVMSAYFPSGSSGEERQEFKMEFLGDFLPYVSELRKSRPNLIISGDYNICHKAIDI
                     HNPVSNKNSSGFLPEEREWVDRFVESGFIDTFRHFNQDPHQYTWWSFRANARAKNLGW
                     RIDYHMATASLEKRLSRSVILPEAKHSDHCPVILEIED"
     gene            2697..6233
                     /locus_tag="PK610_00770"
     CDS             2697..6233
                     /locus_tag="PK610_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34484.1"
                     /translation="MVFRRHKVNQGSLITCAEGPFPVILCSKTKTIRSLVHIILFLVL
                     GHSVQAQSNLRNRLVINSGDTLTIDTLSMVPGSIQVFYQGNFLDTSLFKIDPLKGTMH
                     LDIPTGDTVRILYRVFPQNLHAEFAHKKSGNREPDIQQEKNSFRFQPGKATDDLFGGD
                     GLNKSGSISRGVNFGNNQDLSVNSSLNLQLNGKISDRLSLMASVTDDNIPIQPDGNTQ
                     QLQDFDQVFIKVYDENSSLTAGDFWMKKPVGHFMVYNKRAQGASFSTLQSNTEKGQSF
                     ETRTSAALSKGKFARNLIPGREGNQGPYRLRGAENETFIIVLAGTEQVYIDGQLLKRG
                     QENDYIIDYNTAEITFTPNRLITKDRRIVVEFQYSDKNYARSIFESTNTWKFRNTGIH
                     LNIYSEQDSKNQPLQQNLSDSAKSLLASVGDSVYIAVFPSQDSVGYDPNRVLYRKTDS
                     LGYDPVFVFSSHPDSAVYAVRFSFVGEGRGDYVQSNFTAIGRTFRWVAPDTVGSQIVR
                     RGNFAPVEILIAPKKRQMISTGIDHRFGKKGRFRTELALSKTDQNTFSSLHKSDDQGF
                     GIKLAYENDYSVFPRKKDLSLYTKADVEMVDARFRSIERYRSVEFERNWNVLNRPLQS
                     SQLLADIEIGIRNKQERLGYGFQSFLSGKEYNGMLHRLNGDLRRKRWAGYFNGSYLEA
                     KGSSSSNFIRHKSQIARTIGKFSIGYRDEHELNRFYTATDSLRTGSYQFHEWEAFFET
                     ADTARNRIHFAYRERIDKLYDTTKLGKAAHARQYTAGFTLASNASHRLRAHAGYRELQ
                     IVNPLLTNQAPERTLVNRVEYDLRAWKGAVTANTFFEVGTGQELRKEFIYLEVPAGQG
                     IYAWVDYNGNGIKDLSEFEIAAFPDQARFIRSFTPTNEYVKTYTNQFSQSLLLNPAAV
                     WRSKKGWKKALTYLSNQTAYRVDRKTNSENETDRFNPFLREIADTSLLSLNSSFRNTI
                     FINRTGAVFGLDHTYQDLRSKSLLTNGFDSRIQRSHLVKLRWNITPEYSIIIEGERGE
                     KGSTSDFLSSRNFIIGYDKINPRLNFQPNTRLRITASYKYSNKQNKPEYGNESAELHD
                     IGTELRYNVVNKGSLLINMNHIRIRYVGANGTPIAFDMLEALQPGMNYTWSSSWQQNL
                     SKNMQLSLSYNGRKSETSKIIHTGGVQVRAFF"
     gene            6246..7133
                     /locus_tag="PK610_00775"
     CDS             6246..7133
                     /locus_tag="PK610_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRJ34485.1"
                     /translation="MTKSPGKIALIAPARKMEAADIEIGKQCLQKWGFQLTEGAHLYS
                     QHNQFAGTDNERILDFQNALNDPTVTILLCARGGYGSLRIVDAIDFTAANNKIIAGYS
                     DITVFHNHLISKHNIPSLHCTMPVNMASNSSEALESLKDALLRKNLHYEIAAHSLNKT
                     GDAEGVLFGGNLSLIYAMNGSSSLPNPEGKILFLEDLDEYLYHIDRMMLNLKRSGILQ
                     KIAGLIIGGFTGMKDNPTPFGKTAEQIIQEHTQEYNYPVCFGFPAGHVNDNRALIMGG
                     TTRLTVNPDKVIVRSTWEQ"
     gene            complement(7183..7836)
                     /locus_tag="PK610_00780"
     CDS             complement(7183..7836)
                     /locus_tag="PK610_00780"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HRJ34486.1"
                     /translation="MKFFSFQKLMLPAILLFGLTLTGAGSVKANFEGVIEFTKKTGNT
                     EVKYKYFVKGDKMRIEDFGTDGTLQGIMLVDAAANKVIGISPERKLWMDMPNNRQRKD
                     VKLDIQKTNNSKEIAGYKCTEWKVSCKDEDRVITYWLANDQFTFFMPMLKTLNRADKL
                     SLYFLKLNGADGMFPMLGEEKKADGTLITTLKVSTVKKQSLDNSIFDIPQGYSKYDK"
     gene            7883..8266
                     /locus_tag="PK610_00785"
     CDS             7883..8266
                     /locus_tag="PK610_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRJ34487.1"
                     /translation="MQKLFQTMNDKKKLGNKGEELALVHLLSKGYEILEVNWKSGHLE
                     VDIIARDADRLVFVEVKSRTSAVFGEPVEFVKPTQQNRIFRAANLYYEQKASELEARF
                     DIIGLLFMPEGFEIVHLEDAFYPIA"
     gene            8326..10392
                     /locus_tag="PK610_00790"
     CDS             8326..10392
                     /locus_tag="PK610_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRJ34488.1"
                     /translation="MKRQSSRRPTTGKGPRRSESKSKSGGRSQRDDNKGEKRSFGKKE
                     SGFSSDRKKSYGKKDDSFGSDRKRSYGKREDDGGEKRSYSRRSDEGGEKRSYGKREGG
                     FSSDRKKSYGKKEDSFGSDRKRSYGKREDEGGEKRSYSRRSDDGGEKRSYGKRDGGFS
                     SDRKKSYGKKEDSFGSDRKSSFGKREDEGGEKRSYSRRSDDGGEKRSYGKRDGGFSSD
                     RKKSYGKKEDSFGSDRKRSYGKRDGDTGEKRSYSRRSDDGGEKRSYGKRDGDAGEKRS
                     YSRRSDDGGEKRSYGKRDGDTGEKRSYSRRSDDGGEKRSYGKREEGFGNDRKKSFTLK
                     KDDGTAEKRGYGRDKTDNSGDRKRSYAKSGEEFSGESNPETEGSSYFSGQTRKGGFGR
                     KKEDGEIKKPYIEKEFSDDEEFDFKELSKEKKSFKKSASAYNDMVNRSKEKKKTTKPH
                     DDGKIRLNKFVANTGICSRREADTLIATGVIRVNGQVVTELGTRILPTDKVHFHDQLL
                     SQEKMVYVLLNKPKDYITTTDDPEKRKTVLDLVENATRERLYPVGRLDRNTTGLLLMT
                     NDGELTDKLTHPRNNIKKIYHVMTDKNVKREDLMKLKEGFELEDGFIKVDDAQFVGDA
                     SNKRDIGVEIHSGKNRIVRRMFEHLGYDVIKLDRVMFAGLVKKDLPRGKWRFLSAKEV
                     SFLKML"
     gene            10400..11368
                     /gene="trpS"
                     /locus_tag="PK610_00795"
     CDS             10400..11368
                     /gene="trpS"
                     /locus_tag="PK610_00795"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009418979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRJ34489.1"
                     /translation="MPRILTGIQSSGVPHLGNILGAIQPAIELCRQPGNDSFIFIADM
                     HSLTSLKDGAIRRNNTYAVAASWLAFGLDVQRTVFYRQSMIPEVCELAWYLNCFTPFP
                     MLANAHSFKEKSDKLSDVNAGLFTYPVLQAADIVLYDAEYIPVGKDQVQHLEISRDIA
                     EAFNRIIGETFVVPEAKVDERVMIVPGTDGQKMSKSYKNFIDIFLPEKELKKQIGTII
                     TDSTPLEEPKNPDTCNVFKLFQLIANKEQTSALREKYLAGNFGYGHAKQELLGVILEK
                     YAEPRSLFVHYMNSLPELENQLKIGEEKARHIAHAKMAVVRKKLGF"
     gene            11417..12064
                     /locus_tag="PK610_00800"
     CDS             11417..12064
                     /locus_tag="PK610_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HRJ34490.1"
                     /translation="MFILALLMLRIPAIGPYFRGLNTMFHESGHAFFALLLTGKFSKV
                     DLHADTSGSCKYTISGWLRDSLINLGGYPFGTAAAVTMFYFLHKGNTDYVFYGLAGWL
                     ILNLLFWIRNRFGWFWILTYGAMLAGCYYLQNEALTYYFLFFSAALNLMEAVYSSLVI
                     LWLSVDEPGHAGDTTNLRQITYVPAVVWGLLFATSTLYGAGWVVQILFGWNILPF"
     gene            complement(12065..12775)
                     /locus_tag="PK610_00805"
     CDS             complement(12065..12775)
                     /locus_tag="PK610_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ34491.1"
                     /translation="MKKIFALVVLLSITQPVFSIERVYLLHGHGSDPRIFSGLKLDSS
                     RYDTVSIRLPLPAHLELMPDYVKRIIPMIDTTGLFSFVGVSLGGMVAVELSKHLPVRR
                     VILVSSASCKEELPLRYRSMKHIEVYRCFSGGFYKAMAKPAQLLFEPERRENADLFDA
                     MLQDKDPVFLKRAIHLIVNWDGVCGSDERIVKIHGTKDHTIPARTANPDIRLKNSSHM
                     MMLTRSDVLSEIIQKSLD"
     gene            complement(12807..13589)
                     /locus_tag="PK610_00810"
     CDS             complement(12807..13589)
                     /locus_tag="PK610_00810"
                     /inference="COORDINATES: protein motif:HMM:NF007757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRJ34492.1"
                     /translation="MGNTNELKVCIVQTELDWENPEANRMRFDRLLQNVQADLIVLPE
                     MFSTGFSMQPEKLAEAMSGDTVQWMLTTARKKNAVLTGSVIIKAEDQYVNRLLWINPS
                     GTIEYYDKRHLFRMGNEHEHYSAGSTKIFPEVKGWRICPLICYDLRFPVWSRNSSPFY
                     DVLIYVANWPELRREPWMTLLSARAIENQSYVVGVNRIGTDGRGIAHSGDSCVMDAKG
                     MRISSDSYNCELVETVLLKRNELLDFREKFPVLLDGDDFSIR"
     gene            complement(13589..14749)
                     /locus_tag="PK610_00815"
     CDS             complement(13589..14749)
                     /locus_tag="PK610_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019670955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HRJ34493.1"
                     /translation="MAHSSSPVIISKLPQVGTTIFTVMSRLAAEHGAINLSQGFPDFD
                     CSPDLLRHVSKAMNDGHNQYAPMQGLLSLRETLCAKMNRAYNVNYHPETDITITAGGT
                     QAIYSAISAFIREGDEVILFAPAYDCYQPAIELNGGVAVWSTLKQTDFSIDWPEVKKL
                     INHRTRMIIINTPHNPSGTLLKKEDLEQLEKITKGTDILVLSDEVYEHLVFDGQEHNS
                     VCKFPGLKERSILIFSFGKTFHVTGWKMGYALAPANLMAEFRKAHQFIVFAVNTPMQH
                     GLNAYLQNEENYNSLSAFYQQKRDFFLDGIKGSRFSFRPSGGTYFQLLDYSAIADEKD
                     TDFSIRLTKEKKLASIPISVFYHEPVNTKVLRFCFAKKEETLKRAIDVINSI"
     gene            14856..15092
                     /locus_tag="PK610_00820"
     CDS             14856..15092
                     /locus_tag="PK610_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34494.1"
                     /translation="MRLGSILLFVTGSLFLASCGPEADAREVCACFHEVHQLSEEEGT
                     EKMNACLKQLDRFRSRYKNSGDEIEFETALDRCR"
     gene            15052..15303
                     /locus_tag="PK610_00825"
     CDS             15052..15303
                     /locus_tag="PK610_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34495.1"
                     /translation="MKLNLKLLLIVAVNTCFLSCENAAEESANKWCRYNEKVKTASNE
                     KEQLQWAEKARELEEEIKTEFGSDPESMNIIYTITNECD"
     gene            15290..15547
                     /locus_tag="PK610_00830"
     CDS             15290..15547
                     /locus_tag="PK610_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34496.1"
                     /translation="MNAIKVCLLFVIFLTVSCSTRDPKTLAREYCACFKEGLNDPNRM
                     NECAELAREHQELLPKDPEAAKIYGEEIIRCAVYDQNEKTR"
     gene            15558..15788
                     /locus_tag="PK610_00835"
     CDS             15558..15788
                     /locus_tag="PK610_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34497.1"
                     /translation="MKNLIYTSAVLFMFASCKKDDPQANAEQYCECMKKAAADHSYLP
                     DCEVMGNELLKKYPEGSEGRSTVESVMLYCGK"
     gene            complement(15921..16670)
                     /locus_tag="PK610_00840"
     CDS             complement(15921..16670)
                     /locus_tag="PK610_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HRJ34498.1"
                     /translation="MKLNLKIWRQKNREDKGKMVDYSIDHVSPDMSFLEMLDVLNEQL
                     INKGDDPIAFDHDCREGICGMCSLFINGRAHGPNYGVTTCQLHMRSFKDGDTIFIEPW
                     RAQAFPVIKDLAVDRSAFDRVIQAGGYVSANTGGAQDANCLPVPKENADKAFAAATCI
                     GCGACVATCKNGSAMLFVSAKVSQLALLPQGKAEAQQRVLNMVAQMDAEGFGNCSNTG
                     ACEIECPKGISLENIARMNREFMKASILSEK"
     gene            complement(16698..>17834)
                     /locus_tag="PK610_00845"
     CDS             complement(16698..>17834)
                     /locus_tag="PK610_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRJ34499.1"
                     /translation="NDGRIWVPKKKEDAVAIQQGKKLGKDIAEDDRDYYLERRYPAFG
                     NLVPRDVASRAAKERCDAGFGVNATGQAVFLDYAAAIERYGKIEANKHNMTNPTPEQI
                     RAMGKDVVKEKYGNLFDMYKQITGEDPYETPMKIYPAVHYTMGGLWVDYELMTTIPGC
                     YALGEANFSDHGANRLGASALMQGLADGYFVIPYTIGNYLADDIRTGKIPTNTPEFDA
                     AEKAVKDQINFFVNNKGTKSVDYFHKRLGKIMWDKCGMARNEKGLKEAIQEIRQLREE
                     FWKEVRVPGSNDEFNPELEKAGRVADFLELGELMCMDALTREESCGGHFREESQTEEG
                     EAKRDDNRFSFVSAWEYKPDIKDEVLHKEELKFEEIKVVARSYK"
BASE COUNT         5327 a         3891 c         3789 g         4827 t
ORIGIN      
        1 gaataaaaaa gggaacgcta gaaacgttcc cttttcaaat ttgtggtgca ggattatttt
       61 ttctttttct tctttttccc tttttcttca atagcaaaat atacacgcga aagatcgcgg
      121 tattccattg cattaatcgg gaaattacgc acattagttt gtaccagacg gatgtaattg
      181 tcgtaataca gtggcattac cacagcatca tccacaatca actgatcaca cttagcgaac
      241 agctccatac gctctttttc atcagttgtg cgtaatgctt tgctgaacat ttcgtcgaat
      301 gcatcacttc tgtaacgtgt gaagttgata tacgatttgt cggaagggtt ttcaggaaca
      361 ttctttccgt aaaacaagtt gaggtaattc tcaggatccg gataatccgc tacccaggca
      421 gaacgccaaa aatcagcctt gccggattca aactcctcaa tgagctgatt aagcggcatg
      481 acatccagct taactgttac cccaaggttt tctttaagca tactttggat ggcttcagca
      541 agcaattcat tagtagtacc accggagttt agctgaagtg taattacagg aaaccctctt
      601 ccgttgggga aaccggcttc agacattaat ctgcgggctt tgtcaggatc gaaattaaat
      661 cctttgatat ttcctgatgg ataattgggc aatgatggag gaacgattcc aaatgaagca
      721 ggagtaccct cgccctgcag tgtataggtt acaagttttt cgcgatcgat tgcatggtta
      781 aatgcctgac gaactcgctt atcttcaaaa acttttgagg tgctcaggaa agcataaaac
      841 tgaatggata gcgcttctac ctgttgcaat tcgaattcaa cgttaccacc ctggcgagcc
      901 tcgtccatag aaccgagtac agaagagatt tcctctaccg gcagtttcca aaccatatcg
      961 aggttctttt tacggaactc catcagctct gttttctttt ccttatggaa agtgaactta
     1021 acaccctgca aatacggtaa ctgatttcca tgttcatcct gctcccagta attctcattc
     1081 ttttcaagca aaatcatttg accatcacgc aaagacttta tcacgaaagg acctgtaccc
     1141 acaacctttg tgcgcatatc atccttgtat ttttcgtaag cctcacgtgg atatacccaa
     1201 catccggaat gtgcgatgat tttaggaaac gctgagaatg aaaaattcaa ctcgatgctg
     1261 agtgtataat catcaatgac tttaattcct gcaacaccac ctgcaggaga tgcttttcca
     1321 ccggaagttg cagcatagtg ttcatttgca ccttttacct tgtccttaaa caaccagtaa
     1381 actttattat cacctgatgc agtacagaga cgatcaaaac agtatttaaa atcattagca
     1441 gtgacttcac gtgcagcacc accaaaacat tcatcctcat gaaactttac tcctttgcga
     1501 atggtgaagg tgaaaagtgt ggcgtcttca tttacatcaa atgactgtgc aatacaaggg
     1561 acaacttcga gactttgctg atcgaatttc agcaagccct gatacatttg gctggcgatg
     1621 tgatgtgaag agatgtcgtt aatactaagc ggaaagaggt ttttaaaatc ttcaacctca
     1681 ttgatcttga agataccgcc ataaacgact cctcctttgg cttcaaccat tttgagttca
     1741 tcacctttct ttccgccacc gcaggacgcc aaaatcacag aaaggccaac gaccggtaaa
     1801 agtttcttta tcattgctta cttggtgtta gggacattgg caaatatata caaataatca
     1861 ttgcaggaaa gggcttatgc aggaaggcaa attagctgga taaacggcta atcttcaatt
     1921 tccaaaatca ccgggcagtg gtcggaatgt ttcgcctcag gcaatattac cgatctgctc
     1981 aatcgctttt ccaatgatgc tgtagccata tgataatcaa tacgccaacc cagattcttt
     2041 gctcgtgcat tcgctctgaa actccaccag gtgtattgat gcggatcctg attaaaatgc
     2101 ctgaaggtgt cgatgaaccc actttccaca aatcgatcca cccactcacg ttcttcgggt
     2161 agaaagccac tgctgttctt gttggaaaca gggttatgga tatcaatagc tttgtggcaa
     2221 atattgtaat ctcctgaaat gatgaggttc ggcctgctct ttcgtaactc ggatacataa
     2281 ggaaggaaat caccaagaaa ctccatctta aattcctgtc gttcttcccc tgaacttcca
     2341 cttgggaaat aggcgctcat aacagaaaga tcaccaaaat cgcatcgcag gatgcgccct
     2401 tccctatcgt actttggcat tccacagcca atttcaacgt tatccggttc cttcttgctg
     2461 aatatcgcaa caccgctata cccctttttt tcagcaggat gccaatgggt gaaataccca
     2521 agttcctcaa aaggacgaat atcaatttgt tcaggcgtcg ccttgatctc ctgcaggcaa
     2581 acaatatcag ggttaaccga ctttagccac tcgatccacc cctttgagat ggcagagcgc
     2641 aaaccgttga tgttgtaggt aataatgcgg gttttcattg caaaaaatgg ccgagaatgg
     2701 tttttcggag gcataaagtt aatcagggaa gtctgattac atgcgctgaa ggtccttttc
     2761 ccgttatttt gtgcagtaaa acgaaaacga tccggtctct tgtacatatc attctgtttt
     2821 tggttcttgg ccattcggta caagcgcaaa gtaatcttcg taaccgactg gtgatcaatt
     2881 cgggggacac attgactatt gataccttga gtatggtgcc gggaagcatc caggtatttt
     2941 atcaaggcaa cttcctcgat acttctcttt tcaaaattga tccgctaaaa ggaacgatgc
     3001 acctggatat tcctacaggc gataccgttc ggatattgta ccgcgttttt ccacagaatt
     3061 tgcatgcaga atttgcccac aaaaaatcag gaaaccggga gcccgacatt caacaagaaa
     3121 aaaactcctt tcgctttcaa cccggaaaag caacagacga tctcttcggt ggtgatggac
     3181 tgaacaaatc aggaagtatt tcaagaggag taaactttgg gaataatcag gatttatctg
     3241 tcaactcatc ccttaacctg cagctcaatg gaaaaatatc agaccgattg agtcttatgg
     3301 cctctgttac cgacgacaat attccaattc agccggatgg aaacacccaa caactgcagg
     3361 attttgacca ggtattcata aaagtatacg atgaaaactc atcgcttacg gccggtgatt
     3421 tctggatgaa aaaaccggtt ggccacttta tggtttataa caaacgtgca cagggagcgt
     3481 cgttcagtac gcttcaaagc aatacagaaa aagggcaatc cttcgaaaca agaacaagtg
     3541 cggctttatc caaaggaaaa tttgcgagaa atcttattcc gggcagagaa ggaaatcaag
     3601 gtccgtaccg tttacgcggt gcagaaaatg agacattcat tattgtgctt gcaggaacgg
     3661 aacaggtcta tatcgacggt caattattga aaaggggaca ggaaaacgat tatatcatcg
     3721 attacaatac agcagaaatc acatttacac ccaaccggct tattaccaaa gaccgaagaa
     3781 ttgttgtaga atttcagtac tccgacaaaa actatgcgcg ttcgattttt gaatctacca
     3841 acacctggaa atttcggaat acaggaattc atttaaatat atattcggaa caagacagca
     3901 aaaaccaacc ccttcaacaa aacctgagcg atagtgcaaa atcgcttctg gcttctgttg
     3961 gcgacagtgt ttatattgct gttttcccct cgcaggatag cgttggctat gaccctaatc
     4021 gtgtgttgta ccgaaaaacc gattcattgg gttatgatcc tgtttttgtt ttctcttcac
     4081 accccgacag tgcagtttat gctgtacggt tttcatttgt tggagaagga agaggtgact
     4141 atgtgcaatc taattttacg gcaattggac gaactttccg ttgggttgct cctgatacgg
     4201 tgggatcgca aatagtacga agaggaaatt ttgcacccgt tgaaattctt attgccccta
     4261 aaaaaagaca gatgatcagt accggtatcg accatcgttt tggaaaaaaa ggtcgcttca
     4321 gaaccgaact ggccttatcg aaaaccgatc aaaacacatt ctcttccctg cataaatccg
     4381 acgaccaggg ctttggtatc aagcttgcct acgagaatga ttattccgtt tttccccgaa
     4441 aaaaagatct ttcgctctat acaaaagcag atgttgaaat ggttgacgca cgattccggt
     4501 ccattgaacg gtaccgctcc gttgagtttg agcgcaattg gaatgtcctc aaccgtccct
     4561 tgcaatcatc gcaattatta gcagatattg aaataggaat tcgcaataaa caggaacgat
     4621 tgggatatgg ctttcaatct tttctttccg gaaaagaata caatgggatg ttgcatcgct
     4681 taaacggtga tttacgaaga aaaaggtggg cggggtattt caacggcagc tatctggaag
     4741 ctaaagggag cagcagcagc aactttatcc ggcataaaag tcagattgca cgcaccattg
     4801 ggaaattcag tattggctat cgcgacgagc atgaactgaa ccgtttctac actgcaaccg
     4861 atagtctgcg aacagggagt tatcaatttc atgaatggga agcgtttttt gaaacagcag
     4921 acacagcacg aaatcgaatt cactttgctt atcgcgaacg cattgataaa ttatacgaca
     4981 ctaccaaact gggaaaagcc gcacatgcac gacaatacac agcaggattt accctcgcat
     5041 ccaacgcatc acatcgatta agagctcatg cagggtatcg cgaattgcag attgttaatc
     5101 ctctgcttac aaatcaagca cctgagcgca cccttgtaaa cagggttgaa tacgatttgc
     5161 gtgcctggaa aggagcggtt actgccaata cattttttga agttggaacc ggacaggagt
     5221 tgaggaaaga atttatttat cttgaagtcc cggcaggaca agggatatat gcatgggtag
     5281 actataatgg aaatggaata aaagatctta gtgaatttga aattgcagca ttccccgatc
     5341 aggcacgatt tatccggtcg tttacgccaa ccaatgaata tgtaaaaaca tataccaatc
     5401 agtttagtca gagtttgcta ttgaatccgg cagcagtatg gcgctcgaaa aagggttgga
     5461 agaaagcgtt gacctacctc tccaaccaaa cagcctaccg tgtagatcgc aaaacgaatt
     5521 ctgaaaatga aacggatcgg tttaatccat ttctgcgcga aattgcagac accagtttat
     5581 tgtcgctgaa ttcatccttt cgaaacacca tttttataaa cagaaccgga gctgtttttg
     5641 ggctcgacca tacatatcag gacctccgat cgaaatcgct gcttacaaac ggattcgact
     5701 cccgaattca gcgatctcac ctggttaagc tacgttggaa cattaccccc gaatacagca
     5761 ttattattga aggggagcga ggagaaaagg gaagcacttc cgactttctg agctcaagga
     5821 atttcatcat tgggtatgac aagattaatc cccgactcaa ttttcaaccc aatacccgtt
     5881 tgcggattac tgcatcctac aaatacagca acaaacaaaa caaacccgaa tatggaaatg
     5941 aatcggcaga acttcatgac ataggaaccg aacttaggta taatgttgtg aacaaaggga
     6001 gtttgttgat caacatgaat catattcgca tacgttatgt tggggcaaac ggaacgccca
     6061 ttgcattcga catgctggag gcattgcagc ccggcatgaa ttatacctgg agcagcagtt
     6121 ggcaacaaaa cctttcgaaa aatatgcagc tcagcctgag ttacaacggg agaaaatcgg
     6181 agacttcgaa aatcattcac acaggcggag tgcaggtcag agcctttttt tgagatattt
     6241 gagctatgac aaaatccccg gggaaaatcg cactcattgc gccagctcga aaaatggaag
     6301 ctgccgatat agaaatcgga aaacaatgtc ttcaaaaatg gggatttcag cttactgaag
     6361 gtgcgcatct ctattctcaa cacaatcagt ttgcagggac ggacaatgaa cgaattctcg
     6421 attttcaaaa tgcactgaat gatcctaccg taacgattct tctttgtgcc aggggtggtt
     6481 acggaagctt aagaattgtt gatgcaattg attttactgc agcaaacaac aaaataattg
     6541 cagggtatag cgacatcact gtttttcaca accatttaat aagcaagcac aacatacctt
     6601 ccctgcattg tacaatgccg gttaatatgg caagcaattc gtctgaagca ctggagagtc
     6661 tgaaggatgc attactaaga aaaaaccttc actacgaaat agcggctcat tcgctcaata
     6721 aaacaggcga tgctgagggt gtcttattcg ggggcaatct ttcattgatt tacgctatga
     6781 acggaagctc ctcccttcca aaccccgaag ggaaaattct ctttcttgaa gacctcgatg
     6841 aatacctcta tcatatcgac cgcatgatgc taaatttaaa gcgaagcgga atacttcaaa
     6901 agattgccgg actcatcatt ggcggattta ccggaatgaa agacaatcct actccatttg
     6961 gaaaaactgc agagcaaatt attcaggaac atacacaaga atacaattac ccggtttgtt
     7021 ttggctttcc tgcaggtcat gtgaacgaca accgcgcact gataatggga ggaacaaccc
     7081 gtttaacagt taacccagat aaagtgatcg tgcgctcaac ctgggagcaa taaaaaaagc
     7141 ggaagaattt tctcccgctt ttcgttggtg aaattccaac tgttacttat cgtatttcga
     7201 atatccctgc ggaatatcaa aaatcgaatt atcaagtgat tgtttcttaa ctgtagaaac
     7261 ctttaaagtg gtgatcaagg tgccatcggc cttcttttcc tcacctaaca tagggaacat
     7321 tccatccgca ccattaagct tcaaaaaata cagggaaagc ttatcagcac ggttaagggt
     7381 tttaagcatg ggcatgaaaa aagtgaactg atcgtttgcg agccaatacg taattacacg
     7441 gtcttcgtcc ttacaactta ctttccactc ggtacacttg tagcctgcaa tttctttgga
     7501 gttgttggtt ttctgaatat cgagttttac atccttacgc tgacgattat tgggcatatc
     7561 catccacaat ttacgctccg gagaaatccc gatcacttta tttgcagcag catcaaccaa
     7621 catgataccc tgaagcgttc cgtcagtacc aaaatcttcg atacgcatct tatcgccctt
     7681 aacgaaatac ttgtatttaa cctcggtatt tcccgtcttt ttggtgaact caattacgcc
     7741 ctcaaagttg gcctttacag aacccgcgcc ggtcaaggtg agaccgaaca gtaaaattgc
     7801 aggcaacatt aacttttgaa aactgaaaaa cttcataatc tgtgatttaa ttttaaagat
     7861 acaaaaatag tctttaacca gattgcaaaa gctgttccaa accatgaatg acaagaaaaa
     7921 attgggtaat aaaggtgagg aactggctct ggttcacctt ctttcaaagg gctatgaaat
     7981 tctggaagtc aactggaaat cgggtcacct tgaagtggat atcatcgcaa gagacgctga
     8041 tagactggtc tttgtggaag tgaaatcccg aacttcagct gtttttggtg agcccgttga
     8101 atttgtgaag cccacccaac aaaacaggat ctttagagct gcaaaccttt actatgagca
     8161 aaaagccagt gaactggaag cgcgctttga tattatcgga ctccttttca tgcccgaagg
     8221 ctttgaaatt gttcatttgg aagacgcatt ctacccaatc gcctagtgtc cggcaaacaa
     8281 cttgggtaaa atagccgtac ttttgcgcct tattaataaa gttccatgaa aagacaatca
     8341 tcgcgcaggc ccacaaccgg taagggtccc cgcagatctg aaagtaaaag taaatccggc
     8401 ggccgcagtc agcgcgacga caacaaaggg gaaaaacgct cattcggcaa gaaagaaagc
     8461 ggattcagtt ccgatcgcaa aaaatcatac ggcaaaaaag acgacagttt tggttccgac
     8521 agaaaacgtt cctacggaaa acgtgaggac gatggcggag aaaaacgatc ctattcgcgt
     8581 cgcagcgatg agggtggtga gaaaagatcc tacggtaaac gtgagggcgg attcagttcc
     8641 gaccgcaaaa aatcatacgg taaaaaagaa gacagcttcg gctctgacag aaaacgttct
     8701 tacggaaaac gtgaggacga aggcggagaa aaacgatcct actcccgtcg cagcgatgac
     8761 ggtggagaga aaagatctta cggcaaacgt gatggcggat tcagttctga tcgcaaaaaa
     8821 tcatacggta aaaaagaaga cagcttcggc tctgacagaa aaagttcttt cggaaaacgt
     8881 gaggacgagg gcggagaaaa acgatcctac tcgcgccgca gcgatgacgg tggagagaaa
     8941 agatcttacg gaaaacgtga tggcggattc agttccgacc gcaaaaaatc atacggtaaa
     9001 aaagaagaca gcttcggctc tgacagaaaa cgttcttacg gaaaacgtga tggtgatacc
     9061 ggtgagaaac gttcatactc cagaagaagt gacgatggtg gagaaaaacg ttcgtacgga
     9121 aaacgtgatg gtgatgccgg tgagaagcgt tcatactcca gaagaagtga tgatggtgga
     9181 gaaaaaagat cgtacggaaa acgcgatggt gataccggag agaaacgttc atactccaga
     9241 agaagtgacg atggtggaga aaaacgttcg tacggaaaac gtgaggaagg attcggtaat
     9301 gatcgcaaga aatcctttac actgaaaaag gatgacggaa ctgcagaaaa acgcgggtat
     9361 ggtcgtgata aaaccgataa ctccggcgac aggaaaagaa gctatgcaaa atccggagag
     9421 gaattttccg gtgaaagcaa tccggaaaca gaaggtagtt cctatttcag tggacaaacc
     9481 cgaaaaggtg gttttggtcg taaaaaagag gatggggaaa ttaagaagcc ctatatcgaa
     9541 aaggaattct cggatgatga agaatttgac ttcaaagaac tgagtaaaga gaaaaaatct
     9601 ttcaaaaaat cggccagtgc ttacaatgac atggtaaatc gctcgaaaga gaaaaagaaa
     9661 actaccaagc cgcacgatga tggtaaaatt cgcttaaata agtttgtagc caatacagga
     9721 atttgttcac gtcgtgaagc cgatacattg attgcaaccg gtgttatccg tgtaaacgga
     9781 caggtagtaa cagagttagg cacacgcatt ttaccaacag acaaagtcca ttttcacgac
     9841 caattattga gtcaggaaaa aatggtttat gttcttctga acaaacccaa agactacatc
     9901 actacaactg acgatcctga aaagcgcaag accgtacttg atcttgttga gaacgcaacc
     9961 agggaacgct tgtatccggt tggacgtttg gatagaaata caacaggatt attattgatg
    10021 accaacgacg gagagcttac agacaaactc acccatccca gaaacaacat caaaaagatc
    10081 tatcatgtaa tgacggataa aaacgttaag cgtgaagatc tgatgaaact gaaggaaggc
    10141 ttcgagctgg aagacggatt tattaaagtc gacgacgctc aatttgtggg cgatgccagc
    10201 aataagcgtg atatcggtgt tgagattcat agcggaaaaa acagaatcgt tcgtcgtatg
    10261 ttcgaacatt tgggatacga tgtgatcaaa cttgaccgtg taatgtttgc cggattggtg
    10321 aaaaaagatc ttccacgcgg aaaatggaga ttcctttctg caaaagaagt ttcgttttta
    10381 aaaatgcttt gatctaagta tgccgcgaat actcacagga attcagagct caggtgttcc
    10441 acatctcggg aacatattgg gagccattca gcctgccatt gaattatgtc gtcagccggg
    10501 aaatgattcg ttcattttta ttgccgacat gcattcgctt acctcactaa aagatggtgc
    10561 cattcgacgc aacaacacct atgcggttgc tgcttcctgg cttgcttttg gattagatgt
    10621 tcaacgaaca gtattctatc gtcagagcat gattccggaa gtgtgtgaac ttgcatggta
    10681 cctcaactgc tttacgccat ttccaatgct tgccaatgcg cattcgttta aggagaaaag
    10741 cgataagtta tcggatgtca atgccggttt gtttacttat cctgttttgc aggcggcaga
    10801 tattgttttg tatgacgctg aatatattcc tgttggaaaa gaccaggtgc agcaccttga
    10861 aatttcacgc gatatagcag aagcctttaa tcgcatcatt ggagaaacct ttgttgtgcc
    10921 cgaggcaaaa gtagatgaac gcgtgatgat cgttcctggt acggatggac aaaaaatgag
    10981 caagagttac aaaaacttca tcgatatttt tcttcccgaa aaggaattga aaaaacagat
    11041 tggaactatc attaccgact ctactccact agaggaacca aaaaatcctg acacctgcaa
    11101 tgtcttcaaa ctttttcaac tgatcgccaa taaggaacaa acatcagcct tacgcgaaaa
    11161 ataccttgcc ggaaattttg gttatggtca tgccaaacag gaattactcg gcgtgatact
    11221 tgagaaatat gctgagccac gttcgctatt tgtgcattac atgaattctc ttcctgagtt
    11281 ggaaaaccaa ttaaaaattg gagaagaaaa agcaagacac attgctcatg ctaaaatggc
    11341 tgtagtcaga aaaaagttag gcttttaaaa gaatccgatg cttgattttg ctccccggga
    11401 ataccaatat gcaggcatgt tcatccttgc gctcctcatg ctgaggatac cggcaatcgg
    11461 cccatacttt cgcggtttaa ataccatgtt ccatgaaagc ggacatgcat tcttcgcatt
    11521 attgcttaca ggtaaattca gtaaagtcga tcttcatgcc gacacctccg gatcttgcaa
    11581 atacaccatt agcggttggc ttcgcgattc gctcatcaat ttaggaggct atccctttgg
    11641 aacagcggcc gcagttacca tgttttattt tcttcacaag gggaataccg attacgtttt
    11701 ttatggtttg gccggctggc taatcctcaa tttacttttt tggatcagga atcgcttcgg
    11761 ttggttttgg attttaacct acggagcaat gctggccggt tgctattatt tacaaaatga
    11821 agctttaact tattactttt tgttcttttc tgcagcctta aacctgatgg aagcggttta
    11881 ttcttcgctt gtgattttat ggctaagtgt cgatgaaccc ggccatgcag gcgacacgac
    11941 aaacctcagg caaatcactt atgtacctgc tgtggtatgg ggacttctat ttgccacaag
    12001 caccctttac ggggcaggtt gggtcgtgca gattcttttc ggatggaata ttttaccctt
    12061 ctgattaatc aagtgatttc tgaataattt cagataacac gtccgaccgt gttagcatca
    12121 tcatgtgcga agaattcttt aaccgaatat ccggattggc agttcttgca ggaatggtgt
    12181 gatctttggt tccgtgaatc ttcactatcc gttcatcgct tccacaaaca ccatcccaat
    12241 taacaattaa atgaatcgcc cgcttaagaa aaacagggtc tttgtcttgt agcatcgcgt
    12301 cgaataaatc ggcgttctct cgtcgctccg gttcaaacaa caattgtgcg ggttttgcca
    12361 tagccttgta gaatcctccg gaaaaacatc gataaacttc tatatgtttc atgcttctgt
    12421 aacgaagcgg caattcttct ttgcaggacg cgctcgatac cagtattacc cttcgaacgg
    12481 gaagatgctt acttagctct accgcaacca ttccacctag cgatactcca acaaacgaaa
    12541 acaaacccgt tgtgtcgatc attggaataa ttcgttttac atagtcaggc attaactcaa
    12601 gatgagcagg aagcggaagc cgaatagata ccgtatcgta ccgcgatgaa tcgagtttta
    12661 atccggaaaa aatacgcgga tcagatccat gcccgtgaag taaatacacg cgctcaatgc
    12721 taaaaacagg ttgtgtaata ctcagcaaca caaccaatgc gaaaattttc ttcatgtaga
    12781 attaacgcta atgagatgca attatttcag cgaatcgaaa aatcatctcc gtccaataat
    12841 accggaaact tttctctaaa atctagtagc tcgttgcgtt ttagcagtac ggtctctacc
    12901 agctcacaat tgtaactatc cgatgaaatc cgcattcctt ttgcatccat aacacaggaa
    12961 tcaccggaat gggcaatccc tcttccatca gtgccaatac gatttacacc aacaacatac
    13021 gactgatttt caattgcacg ggcagaaagc aatgtcatcc atggctcacg acgcaattca
    13081 ggccaattgg caacataaat caacacatcg taaaagggag aactattcct tgaccaaacc
    13141 ggaaaacgca aatcgtaaca aatcagggga cagatcctcc aaccttttac ctccggaaaa
    13201 atttttgtgc ttccggcaga ataatgttca tgttcattgc ccattctaaa caagtgacgt
    13261 ttatcgtaat attcgatagt acccgaagga ttgatccaca gcaagcgatt cacatactga
    13321 tcttcagctt taataattac actaccggta agtacagcgt tcttttttcg tgcagttgtg
    13381 agcatccatt gtacggtgtc gccactcatt gcttctgcaa gtttctccgg ctgcatcgaa
    13441 aatccggtac tgaacatttc aggaagaacg atcaaatcgg cttgcacatt ctgaagcaaa
    13501 cgatcaaaac gcatacggtt ggcttcagga ttttcccaat ccaattccgt ttgcacaata
    13561 caaaccttta attcatttgt gtttcccatc aaattgagtt tattacatca atggcgcgct
    13621 taagtgtttc ttcctttttg gcaaaacaga aacgcaatac ttttgtgttg accggctcat
    13681 gataaaaaac ggagatcgga atacttgcca attttttttc tttggtcagg cgaattgaaa
    13741 aatcggtatc cttttcatca gcaattgccg aataatccaa taactgaaaa tatgtccccc
    13801 ctgaaggtct aaacgaaaag cgactgcctt taattccatc caaaaagaaa tcgcgtttct
    13861 gctgataaaa cgcagacagg gaattataat tctcttcgtt ttgcagataa gcatttaaac
    13921 cgtgctgcat tggagtgttc actgcaaata cgataaactg atgggctttc cgaaattccg
    13981 ccatgaggtt ggcaggtgca agtgcatacc ccattttcca accggttacg tgaaacgttt
    14041 tcccgaatga aaaaattaaa atggatctct cctttaagcc cggaaatttg catacactgt
    14101 tgtgttcctg tccatcgaaa accagatgct catacacttc atccgacaga accaggatat
    14161 ctgttccctt tgtgattttc tcaagttgtt ccagatcctc ctttttcagt aaagtaccgc
    14221 ttggattatg cggggtatta atgatgatca tccgtgtacg atgattgatc agttttttta
    14281 cttcgggcca gtcaattgaa aaatcagtct gttttaatgt cgaccaaaca gcaactccgc
    14341 cgtttaattc tattgcgggt tgatagcaat cgtatgcagg cgcaaacagg atcacctcat
    14401 ctccttcacg aataaatgca gaaatagcag aatagattgc ctgagttccc cctgcagtaa
    14461 ttgtaatgtc ggtttcggga tgataattga cgttgtatgc ccgattcatt tttgcacaaa
    14521 gcgtttcccg gagagataac aaaccttgca ttggagcgta ttggttatgc ccatcgttca
    14581 ttgctttgga tacgtggcga agcaaatccg gcgaacaatc aaaatcagga aatccctgcg
    14641 ataaattgat tgccccatgt tcggcagcaa gccgactcat caccgtgaaa atggtagtgc
    14701 caacctgggg cagcttggaa atgattacag gggaagagga atgtgccata cttcaaacta
    14761 tatacagtaa cgtaaattat tatgaacgct accatgaagt tagtggtttt taacaaagat
    14821 cccatagttc aattcaaatc cttattttcg tattcatgcg cttaggatca atactgcttt
    14881 ttgttacagg aagcctcttt cttgcttcgt gtggtccgga agcagatgcg cgggaagtat
    14941 gtgcctgttt tcatgaagta catcaactga gtgaagaaga gggaacggaa aaaatgaatg
    15001 cgtgtttaaa acaactggac cggtttcgat ccaggtataa aaactccggt gatgaaattg
    15061 aatttgaaac tgctcttgat cgttgccgtt aacacttgct ttttatcctg cgaaaacgct
    15121 gcagaagaaa gcgcgaataa atggtgtcgt tacaatgaaa aggtaaaaac tgcctccaat
    15181 gaaaaagagc aacttcagtg ggcagaaaaa gcacgtgagt tggaagagga aataaaaact
    15241 gaatttggct cagatccgga aagcatgaat attatttata ccataaccaa tgaatgcgat
    15301 taaagtctgt ttgttgtttg taattttcct aacagtaagt tgcagcaccc gcgatcctaa
    15361 aacactggcc agggaatatt gtgcgtgttt taaagaaggg ttgaatgatc cgaatcgcat
    15421 gaatgaatgt gcagaattag caagagaaca ccaggagttg cttccaaaag atcctgaagc
    15481 agcaaaaatt tacggtgaag aaatcattcg ttgtgcggtc tacgaccaaa acgaaaaaac
    15541 gagataagaa catcgccatg aaaaatctga tttatacctc agccgtattg ttcatgtttg
    15601 cgtcctgtaa aaaggacgat ccccaggcga atgctgaaca gtactgcgaa tgcatgaaaa
    15661 aagctgctgc cgatcattcg tatttacccg attgtgaagt catgggcaac gagcttttaa
    15721 aaaaatatcc ggaaggaagc gaagggcgat cgaccgttga aagtgtaatg ctgtattgcg
    15781 ggaaatagat ttattggtga gtggtgaaca gtgaatggtg aacagatttc tattcacata
    15841 tcaccgttca ctttttacca ttcaccaaaa aacaaaaagc cctcttcttg cgaagagggc
    15901 tttttgtttt ttgaaaatag ttatttctcc gaaagaattg atgctttcat aaactcacgg
    15961 ttcatccgtg caatgttttc aagtgaaatt ccttttggac attcaatctc acatgcaccg
    16021 gtattggaac aattcccgaa accttctgca tccatttgtg cgaccatgtt caatacacgt
    16081 tgctgtgctt ctgctttacc ttgtggcaac agagcaagct gagaaacctt cgcagataca
    16141 aatagcattg cagatccgtt tttacaggta gcaacgcatg ctccgcaacc aatacaagtt
    16201 gctgctgcaa aggctttgtc tgcgttctcc ttgggaacag gtaaacaatt ggcatcctga
    16261 gcaccgcctg tgtttgcgct tacataaccg ccggcctgaa taacccggtc aaatgcgctt
    16321 cgatccaccg ccaaatcctt aattacagga aaagcctggg ctctccatgg ttcaataaag
    16381 attgtatcgc catctttaaa agagcgcatg tgcagctgac aggtagttac gccataatta
    16441 ggtccgtgtg cacgaccatt aatgaagagt gaacacattc cgcaaattcc ttcgcggcaa
    16501 tcgtggtcga atgcgatggg atcatctccc ttgttgatga gttgctcatt gagcacatcc
    16561 agcatttcga ggaatgacat atcaggtgaa acatggtcga tggaataatc taccattttc
    16621 cctttgtcct cacggttctt ctggcgccag atttttagat tgagtttcat atctatcgat
    16681 tgaaaattcg aactgaatta tttatacgaa cgtgcaacaa ctttgatttc ctcaaacttg
    16741 agttcttcct tgtgaagtac ttcgtctttg atatcgggtt tgtattccca tgcggaaaca
    16801 aagctgaatc gattgtcatc gcgcttggct tctccttctt ctgtttgtga ttcttcacgg
    16861 aaatgtccac cacaggattc ttcgcgggtg agggcatcca tacacatcaa ttcaccaagc
    16921 tccaggaaat cggcaacacg tccggctttc tccagctcag gattgaattc gtcattgctt
    16981 cccggtacac gaacttcctt ccagaactcc tcacgcaatt ggcggatttc ctgaatggct
    17041 tctttcaagc ctttttcatt gcgcgccata ccgcatttgt cccacataat ttttccaaga
    17101 cgcttgtgga aataatcgac cgacttcgtt cccttattgt tcacgaagaa gttgatttga
    17161 tctttcaccg ctttttcggc agcatcaaac tcaggtgtat tggttggaat ttttccggta
    17221 cggatatcat cagcaagata atttccgatg gtgtaaggaa tcacaaaata tccgtcagcc
    17281 aatccttgca tcagtgcaga agcgccgagg cggtttgctc cgtgatcgga gaagttggct
    17341 tcacccaatg cataacatcc cgggatggtt gtcatcagtt cgtaatcgac ccacaatcca
    17401 cccatggtgt agtgaacggc aggataaatt ttcattggag tttcataagg atcttcgccg
    17461 gtgatctgct tgtacatatc aaacaggttt ccgtattttt ccttcaccac atccttaccc
    17521 atcgcacgaa tctgctcagg tgtaggattg gtcatgttgt gtttattcgc ttcaattttt
    17581 ccgtaacgtt caatcgctgc agcgtaatcg aggaatactg cttgtcctgt tgcattcact
    17641 ccgaaaccgg catcacaacg ctctttcgca gcacgtgagg ctacgtcgcg cggaacgagg
    17701 tttccgaatg caggatagcg acgctcgagg taataatcgc gatcgtcttc ggcaatgtct
    17761 tttccgagtt ttttcccttg ctgaatggca acggcatctt ctttcttctt gggaacccag
    17821 atgcgtccgt cgtt
//
LOCUS       DAOMYG010000011        17811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1093_length_17811_cov_10.377788, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000011 DAOMYG010000000
VERSION     DAOMYG010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 17811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17811
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1093_length_17811_cov_10.377788"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..875
                     /locus_tag="PK610_00850"
     CDS             3..875
                     /locus_tag="PK610_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRJ34500.1"
                     /translation="MILSMTGFGKATGAYGSKKINVEVRSLNSKQLDLNLRMPSKYKE
                     KEMELKSRIAAVVERGKVDVAVYYEDMGEEKLHSINHQLAAAYYADLKQLCSELEIPE
                     GEILGHILRLPDVVTTEKKEVDENEWKTILSIADEALKNFKSFRLAEGSKLQEELNKR
                     VAIILEQLVLVEPFEKERMETVKTKLRKTLEENVNPEIVDKNRFEQELIYYLERLDVS
                     EEKMRLHTHCTYFTETMNADDQSGRKLGFIAQEMGREINTLGSKSNHASMQKIVVLMK
                     DELEKIKEQVLNVL"
     gene            865..1446
                     /gene="gmk"
                     /locus_tag="PK610_00855"
     CDS             865..1446
                     /gene="gmk"
                     /locus_tag="PK610_00855"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008639711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRJ34501.1"
                     /translation="MYSDKAQGKAIIFSAPSGAGKTTIVHHLLSCNLGLEFSVSACSR
                     AMRKGEANGVDYYFLSVEEFRKKIANKEFLEWEEVYTDHFYGTLQSEIERIWKKGHHV
                     IFDVDVVGGINLKKIFGPNALSVFVKAPSIAHLEARLKTRSTETPESIARRMAKAEIE
                     MGYAEKFDHILLNDDLQRAFAEAEQVVKQFLNS"
     gene            1449..2054
                     /gene="nadD"
                     /locus_tag="PK610_00860"
     CDS             1449..2054
                     /gene="nadD"
                     /locus_tag="PK610_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013750735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HRJ34502.1"
                     /translation="MEIGLYFGTFNPIHVGHLIIANYMADYTDLDKVWLIVSPQNPMK
                     EKNTLLADIHRLALVKIAIEDNENLSVSDIEFNLPKPSYTVHTLAYLREKYPQHQFSL
                     IMGEDNLRTLHKWKNHEQILQHHKLYVYPRVLTEQELDELRDYHLESPYKKHPNVIMC
                     DAPVMKVSSSFIRKAIKDKKDIRYLLTDPVQRYIEEMHFYE"
     gene            2173..3891
                     /locus_tag="PK610_00865"
     CDS             2173..3891
                     /locus_tag="PK610_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ34503.1"
                     /translation="MKIFLRIFSVLTIALGSTNAFAQTCTVDYSLTAPGIYPDTLPSG
                     YVGQPYSEDIQFVLPTDTMGYTFLNFHIVSISLPIGLNWQCSNQANGCNYDPAVSVHG
                     CVNVYGTPLLAGQYPITVTVIADLNIVSGYPVSFESYIEILPPNTSSNNNGFSMTNST
                     GCVPITVNFTNNNPGLAAYSWNFGNGNTSTLENPGPQIYNQPGNYVVQYAGYADNTTV
                     QVYNLTQVTINSISTAFSWGWPTELNPDPYFKIKENGVVIHQSNYIQDAFPPHTWPVS
                     IQMDPAKTYVLEVFDEDDYEIIYGGDDLIGSHTMSLSGCTGCAAGSNSTVSYIVNNIL
                     IPPIPSVQSTDTVRVYPYPGVPNIVYDSLAHTLSTDSVQYALQWYFEGTLIPGANAAT
                     YLVPQSGNYQVAAISPGGCQTMSASLTAVYCGNTFVPAVNLNGVVLSTPQNAGDSYQW
                     QFNGINIPGATSSSYTAIVNGNYTLVLTNSFGCSYTSSIVNVPVSVNEIAAQERIAVF
                     PNPAMAQINVVLQSPSAIVLLNMMGEVVMQTGVVMQTQLDISALASGIYFLRTENGST
                     VKVVKE"
     gene            complement(3964..7188)
                     /locus_tag="PK610_00870"
     CDS             complement(3964..7188)
                     /locus_tag="PK610_00870"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRJ34504.1"
                     /translation="MRRLFFLTAMLFLLAPSVYANWFRYPVISPDGKEIVFSCKGDLW
                     KVSSQGGRAMQLTSHSAYDFMPVWSPDGKQIAFASNRFGNFDVYVMSSEGGFPKRLTF
                     HSAGDFPYTFNPEGTAVWFGSARLDDYRNAMFPSKVLHELYAVAVNGGKEKMKLTVPA
                     EALQFSGDGKRILMHDRKGYEDPWRKHHTSSVTRDIVLYDLSTSTFTRQASWKGEDRN
                     PVWAGEKEYYFLSEKSGSFNIWKGIIGGESHQQQLTQHDKHPVRFLSSSQNGVLCYGY
                     DGEIYLFENGNSKKVNISVLADHSENNVSPISVNGNVSEYSLSPNGKEIAFVYRGEIY
                     VSAVAHSTTRRITNTPQQERSVSFSPDGRKLVYAAERAQSWDIYESSITNSGEKYFYN
                     CTQITEKALSTRPEEEFQPAYSPDGKSVAFLEERTTLRVMDLTTGKIVTAMEGSYNYS
                     YSDGDQYYTWSPDSKWLLVEFFEYDRWVSNVGLVSAQGGKKPINLSKSGYGNGSPKFA
                     MGGEMVCWTTDKFGYRSHGSWGAEGDVEAIFLTREAWRKYKASKSESEYESELEKEKQ
                     KPEEKSAETSAKDKKATESKDKKEEVKPLVIDEEGLQDRRTRLTVHSSHLGDYLLNKD
                     ATQLFYMTSYEKGFDIWTTKFKDGETKLLAKVGGSPSAMTFDKEEKFIFFTKNGQLMK
                     VEISNGAITPITVESQFEHRPADERAYMFEHAWRQTKKKFYLADLHGVDWDFYKKEYG
                     KHLYHINNGYDFAELLAELLGEINASHTGATYRYYDPNGDQTSSLGCFYDEEYLGEGY
                     RIAEILPRSPLLASGKIVPGTLITEIDGEPVGQATNIYALLNRKVGKPVVVRFRSGQN
                     SWTETIKAISQGEENDLAYHRYIKQCEKEVEKLSGGTIGYVHVRGMDSPSFRDVYDKA
                     LGVMHTKKALIVDTRFNGGGWLHDDLATLLTGKNYMNFEPRGQKNMGGEPIWKWQKPS
                     CVLMGEGNYSDAHLFPVTYRALNIGKLIGMPVPGTGTAVWWEFMIDGYTRFGIPQVGM
                     RSVKDNHLVENQDLLPDIQVENDWNKVTKGIDEQLAAGVRELMK"
     gene            complement(7277..8512)
                     /locus_tag="PK610_00875"
     CDS             complement(7277..8512)
                     /locus_tag="PK610_00875"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRJ34505.1"
                     /translation="MDFYDLGLVDELLDALDAMNIRNPTPIQQQAIPIALGGHDLIAC
                     AQTGTGKTAAFVLPVMNKLFESNSSGTRALILAPTRELAMQIDQQIEGLSYFVSQSSI
                     AVYGGGVGEVFDRQRKAFDSGVDIIVATPGRLLSFLSSGMLKLDQVKFLILDEADRML
                     DMGFYEDIMRIISYLPKERQNLMFSATMSPRIRQLAQSILKDPKEVSVAIAKPAAGID
                     QQVYLLKEDQKIPMLERILSEEKYQSGIIFSSRKELVKQLFHSLRKKGFACQSFHSDL
                     EQNEREEIMRDFRNRKLRYLIGTDVLSRGIDVEGIDLVVNYDAPPDAEDYVHRIGRTA
                     RASSTGTAITFIDAKDIRRFQRIEQLIAMKVKRMPIPEELGPGIDENAPVDPSAKKKR
                     PFKGKKKRPNGGAPRTNPA"
     gene            8696..10834
                     /locus_tag="PK610_00880"
     CDS             8696..10834
                     /locus_tag="PK610_00880"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HRJ34506.1"
                     /translation="MLRNFLLALLLCVSLPGWAQQLVVDNAGNYSNPNYLTQNILRGG
                     SVAISNVSFSGISSQQIGYFTNGSSATGLSSGMILSTGKAMNAAGTNDSTNTSGGLGS
                     SGSTDPDVMSLANGQAIFDALIYEFDVRPDGNELTLEYILASEEYTEYVCSQEADIFA
                     IIISGPGITGTFSNNGKLISLIPTSSTIPVGINSINNGTPGTFGAPGGCSNSFFSPYY
                     LNNTQNLFEYDGFTTVLQAKADVECNQLYHIRIVLADIGNNGFDTGLFLKERGLYSNS
                     TGNFDEGAFLDSVIVEGCKPFYMVIYNPIGFTGGQTFQVNIGGTAINGTDYDFVPSTY
                     TMNSGDSLLTIVFNPVADGVVEGFESVIVSYFYLNPCGDTVFVNKTFYIRDESPLVVT
                     GLPQNTSICNGQSVLLNVNVSGGFPSYSISWNGMLQNNFNVTPIQDITYVAEVYDLAG
                     CYWTGSFTVDHNPSPIVNAGPDITICSGQPEQLGLQIEGGPGATYNWMPASQLNSGNL
                     PYPTMNPQTTQTFTVGVVTAAGCTGTDQLVVTVLPSPTVNAGPDQSITYLQTTTTLIG
                     SGGGAPSWYPPDGLSCTNCFTPEASPYTTTTYTLYITSGNGCVSSQEVVVEVEIPMDV
                     FVPTAFSPNNDGVNDLLIVRSYTIESMNLKIYDTWGQLLYENTHPDWGWDGTVNGVPA
                     NIGLYVYTLEVVFIQGKGTATFSGEFNLVR"
     gene            10831..11877
                     /locus_tag="PK610_00885"
     CDS             10831..11877
                     /locus_tag="PK610_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRJ34507.1"
                     /translation="MRKVYLSVALWIFCLGISRAQDAHFTQFENSPFFTNPAAAGMIE
                     QSDFRGSLITRNQWRKLVRPTNQTLVSADAPLLRYQGGSRSKSNLGIGLSYGFATMGD
                     SRTRMTNLALALSGMTTVGTRKYLGIGVSAGKGGMRSDLTNATWDAQYNGYKLDESLP
                     TGEAYEGRLKARFFDLSAGLNFLSVNGRTGATTNFGIAYLHANNPKLKNEPLLNGKID
                     PRIVAHFRSEIQLDLKNTMPVFIIPRALVGMQGVHMEIQAGATVFWSAEGTSQITGYR
                     HKTGFEAGGMYRHGDAVGLLAGWKTESYRIGLSYDISISAYGKALNHRGGTEISFIYY
                     AVNKEMRSRKAIYD"
     gene            complement(11973..12455)
                     /locus_tag="PK610_00890"
     CDS             complement(11973..12455)
                     /locus_tag="PK610_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HRJ34508.1"
                     /translation="MAKKTAPKKKAAPKKAAAKKAAPKKAAKKPAAKKAAAKKPAKKA
                     VAKKAAPKKAAAKKAAPKKKVAAKKAAPKKKTARKPNAAFMAPLTPSTVLADVIGNKP
                     VPRTEIVKKIWDYIKKNGLQDKKNKRMINADAKLKALFAGKAQISMFDLAKIVSKNVK
                     "
     gene            complement(12688..13377)
                     /locus_tag="PK610_00895"
     CDS             complement(12688..13377)
                     /locus_tag="PK610_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34509.1"
                     /translation="MKRFVLLSFFLGLLITGIVLVFALPASSRKKETVQSPERPDTLN
                     WTRECPPEGSAKTERIRELNRLKNRISFPHAQDFDTSATMNALLLPGNDEARWNEKKA
                     ARVRAYVIDVKPGGKETCNCGAKEVDDRDTHIDLVLDPMNEVKSKRFIAEVTPPMRSI
                     MKLRGENWTTRGLRDQFLGRWVEVEGWLFLDEEHLNTSENTRPLRENNWRATAWEIHP
                     VTSIKVINRPR"
     gene            13432..15366
                     /locus_tag="PK610_00900"
     CDS             13432..15366
                     /locus_tag="PK610_00900"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRJ34510.1"
                     /translation="MRRSSILRQLLINTLVPVVLVLLAVGVISYRINRQQLEESNRRL
                     REQIVAQTKSILSIHDEALHILEEDLNDRIEMLSDRLLTEYFAKPDSLHTADLFRISA
                     EIGMDTAIEHLYVINDSSVITNTTFAKDLGLDFSKIDTSFVNSFAKMRKEGNLVIDRF
                     GEEMITGKIKKYSFLPTPDGRNIIEMGIYSAMADGLRDKAFKNVSEISANFPEIRNIA
                     SFATVANVKNVHLPDHHYEHYQRAMKEKVPVRVSESEDGIDHYYDYFFLDIQSSSIYT
                     GYLLLIESDNSREKALIRAEVIRFSVLFLVTISLLSLVIYYRSRSIALPIRVLTDKAR
                     VIAGGKLDERVPIKGNNEITDLSQSFNTMIDDLEGMYVNLEKKVKERTHELNHQKELV
                     EEKNKEITDSINYARRIQYALLAHDDLLKENLPEHFVLFMPKDIVSGDFYWACLHPRG
                     NSNDFYLAVCDSTGHGVPGAFMSLLNISFLNEAINEKTIEEPGKILDHTRRRLIENIS
                     QQGQKDGMDGILVRFSEGKISFAAANNPPLIIRNGEEIELAADKMPIGQSDRIDPFTT
                     VIPDLQKGDMFYLFTDGYPDQFGGPKGKKFKYRQLYDLLKEIAHLSMTEQRTILESTI
                     ENWKGDLEQVDDICVIGIRV"
     gene            15619..16125
                     /locus_tag="PK610_00905"
     CDS             15619..16125
                     /locus_tag="PK610_00905"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRJ34511.1"
                     /translation="MIDYSEESLKTLDRFYQALQPDIDRLESAHASKMQCKKGCHACC
                     IDDLVVFGVEAANIVKHHSALVQNEKPHPKGKCAFLNNEGACRIYDVRPFVCRTQGLP
                     LKIINQKGNELRDVCPLNLEGVNLNKIPEKQLLHTNPYEEFLARLQLAIDKGQMERIK
                     LRSLFGKK"
     gene            complement(16262..17236)
                     /locus_tag="PK610_00910"
     CDS             complement(16262..17236)
                     /locus_tag="PK610_00910"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HRJ34512.1"
                     /translation="MGKVLRDMSINVKLLKQICEVPGAPGREQLVRELVMKQVKGHVD
                     SIDIDNMGNITVVKKGKKSDRKVMIAAHMDEIGFMVNHIDEKGFLRFVPLGGFDPKTL
                     TAQRVIVHGKKDLIGVMGCKPIHVMSASEREKVVPLTDYFIDMGMTKKEVEKYISVGD
                     VVTRERELIEMGNCVNCKSLDNRLSVFILLETLRELKNPAYDVYGVFTVQEEVGIRGA
                     SVAALAIQPDFGFGLDITIAYDVPGAKAHEMITELGKGTAIKIMDSSVISDYRMVEFM
                     KKTADKHKIKWQPEILPAGGTDTAGIERMTAGEQSPVVFPFPADMSIR"
     gene            17436..>17811
                     /locus_tag="PK610_00915"
     CDS             17436..>17811
                     /locus_tag="PK610_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine-4-hydroxylase"
                     /protein_id="HRJ34513.1"
                     /translation="MKQNYSNYNKEDQEVWSILFSRQIAQLEEHACTDFRLGLHRLRS
                     ELNADAIPDFDRLNTLLYKLSGWSIEVVPGLISVNDFFMLLSQRKFPSSTWLRKKDQI
                     HYLEEPDMFHDVFGHIPMLALKE"
BASE COUNT         4967 a         4156 c         3706 g         4982 t
ORIGIN      
        1 ccatgatcct atccatgacc ggtttcggta aagccaccgg agcttacggt tccaaaaaaa
       61 tcaatgtaga agtacgttcc ttaaacagca agcagctgga tttgaatctt cgcatgcctt
      121 cgaaatacaa ggagaaggaa atggaattga aatcgcgcat cgctgcagtg gttgaacgcg
      181 gaaaagtgga tgtggcggtg tattacgagg acatgggtga agaaaaactg cattccatca
      241 atcatcagct tgcagcagcc tattacgccg acctcaaaca attgtgcagc gaactagaga
      301 ttccggaagg agaaattctt ggtcacattc tgcgtttacc cgatgttgtt accacggaaa
      361 agaaagaggt cgatgagaac gagtggaaaa ccattctttc cattgcagac gaagcattaa
      421 agaatttcaa atcttttcgc cttgcagaag gatcaaaatt acaggaagaa ctcaacaaac
      481 gcgtcgccat cattctggag caactcgttt tggtggagcc tttcgaaaaa gagcgcatgg
      541 aaacagtaaa aacaaaactg cgcaaaacac tggaagaaaa tgtcaatccg gaaatcgttg
      601 acaaaaaccg ctttgaacaa gaactgatct attacctcga gcgactggat gtgagcgaag
      661 aaaaaatgcg tctacatacg cactgtacct atttcaccga aaccatgaat gcagatgacc
      721 aatccggaag aaaactcgga tttattgcac aggaaatggg gagggagatc aatacactcg
      781 gatcgaaaag caatcatgca tcgatgcaga agattgtggt actgatgaaa gatgaactgg
      841 aaaaaattaa ggaacaggta ttaaatgtac tctgataaag cacaaggcaa agcgatcatt
      901 ttttctgctc catcaggcgc gggtaaaacg acgattgtac atcatttgtt aagctgtaat
      961 ctcggactcg aattttctgt atcggcatgc agcagagcga tgagaaaggg agaagcgaac
     1021 ggcgttgatt attatttcct gagtgtggaa gaattcagaa aaaagattgc caacaaagaa
     1081 ttcctggaat gggaagaagt gtacacggat catttctacg gcacgctgca atcggaaatt
     1141 gagcgcattt ggaaaaaagg tcatcacgtt atttttgatg tagacgtagt tgggggaatc
     1201 aacctgaaaa agatttttgg tccaaacgca ttatccgttt ttgtaaaagc gcccagcatt
     1261 gcccatttgg aagcgcgact gaaaacgcgt tcgactgaaa ccccggaaag cattgcacgc
     1321 agaatggcaa aagcagaaat tgaaatgggt tatgcagaaa aatttgatca tatcctgcta
     1381 aacgacgatt tgcaacgcgc ctttgccgaa gcggaacagg tcgtgaaaca attcctgaat
     1441 tcctgattat ggaaattggt ttgtatttcg gaactttcaa tcctattcat gtaggacacc
     1501 tcatcattgc caactacatg gccgattaca ccgatctgga taaagtgtgg ctcatcgtat
     1561 cgcctcaaaa tccgatgaag gaaaaaaaca ccttgcttgc cgatattcac cggttggcat
     1621 tagtaaaaat tgcgattgag gataacgaga acctttccgt ttcagatatt gaattcaatc
     1681 tgcccaaacc ttcgtacacg gttcatacac ttgcttattt gcgcgaaaaa tatccgcagc
     1741 atcaattttc tctcattatg ggagaagaca atctgcgaac gctgcacaaa tggaaaaacc
     1801 atgagcaaat acttcaacac cacaagttgt atgtatatcc ccgtgtactc actgagcagg
     1861 aacttgatga gctgcgcgac tatcatcttg aaagtcccta taaaaaacat ccgaacgtta
     1921 ttatgtgcga tgcaccggta atgaaagttt caagttcatt tatccgaaaa gcgatcaagg
     1981 acaaaaaaga catccgctac cttctcaccg atccagtaca acggtatatt gaggaaatgc
     2041 atttttatga gtaaaacccg cttttgaaat tcagcgtttt accgaacaac acccaccttt
     2101 cgtcagattt tccctattct tttacttttc atacaagaga ttttgtaaat tacttcttca
     2161 atccaaacga agatgaaaat atttctccgc attttttcag tgctgacaat tgcacttgga
     2221 tctacaaacg catttgccca aacctgcacc gtcgattact ccttaaccgc tccgggaatt
     2281 tatcctgata cactaccctc cggttatgtg ggacagcctt acagcgaaga cattcaattt
     2341 gttcttccta cggacacgat gggatatact tttcttaact tccatatcgt ttccatttca
     2401 cttcccattg gattaaactg gcaatgcagt aatcaagcca acgggtgtaa ttacgatccc
     2461 gccgttagtg ttcacggttg tgtaaatgtt tatggtacac ctctgcttgc aggacagtat
     2521 ccgattaccg ttactgtaat tgccgatctg aatattgtaa gcggatatcc ggtatcgttt
     2581 gaatcctaca tcgaaattct tcctcccaat acttcttcca acaacaacgg attcagcatg
     2641 acaaatagca ccggttgtgt tcccataact gtgaatttta ccaataataa tcccggactt
     2701 gcagcttaca gttggaactt cggaaatggc aacaccagca cattggaaaa tcccggtccg
     2761 caaatctata atcagccggg aaattatgtc gttcagtatg caggttatgc cgataacacc
     2821 accgtacagg tttacaatct tacgcaagtc accatcaatt ccatttctac cgctttctcc
     2881 tggggctggc caacggaatt gaatcctgac ccctatttca aaatcaagga gaacggcgtt
     2941 gtgattcatc agtcgaatta cattcaggat gcttttcctc cccatacctg gccggtgagt
     3001 attcagatgg atcctgccaa aacctatgtg ctcgaagttt ttgacgagga cgattacgaa
     3061 atcatatacg gcggagatga tctgatcgga tctcatacca tgagtctctc cggttgtacg
     3121 ggatgtgctg cgggatccaa ttcaaccgta agttatatag taaataatat attgattcct
     3181 ccaattccaa gtgtacaatc caccgatacc gttcgcgtgt atccgtaccc cggtgttccc
     3241 aatattgttt acgattcact tgcacacacc ttgagcactg attctgttca atatgcattg
     3301 caatggtatt tcgaaggaac gctcattcct ggtgcgaatg ctgctactta ccttgtacca
     3361 caatccggaa attaccaggt agcagcaatc tctcctggag gatgtcagac catgtcggct
     3421 tccctcaccg ctgtatattg cggaaacact ttcgttcctg ctgttaattt aaacggagtc
     3481 gttttatcta caccacaaaa cgcaggcgac agttaccaat ggcaattcaa tggaatcaat
     3541 attccgggag ctacatcatc ctcatacact gctattgtaa atggaaatta cacactcgtt
     3601 cttaccaatt cattcggatg cagttacact tccagcatcg taaatgttcc tgtaagtgta
     3661 aatgaaattg ctgcacagga acgtattgct gtttttccta atcctgcaat ggcacaaatc
     3721 aatgttgtac ttcaatcacc atctgcaatt gtacttctga acatgatggg agaagtggtg
     3781 atgcaaaccg gagtagtgat gcaaacacaa ctcgacatca gcgctctcgc ctccggtatt
     3841 tatttcctga gaacagaaaa cggttccaca gtgaaagtgg tgaaagaata atttgagcgg
     3901 aaattattta ccctcaacaa aaaaccccga agagatcctt cggggttttc tttttgtcgg
     3961 cagttatttc atcaactccc ttacgcctgc ggctaactgt tcgtcgattc ctttggtgac
     4021 tttattccaa tcattctcca cctgaatatc gggtaagaga tcctgattct ccaccagatg
     4081 attgtccttt acactacgca tgcccacctg tggtattccg aaacgcgtat aaccatcaat
     4141 cataaactcc caccaaacag cagttcctgt tccgggcacc ggcataccaa ttaatttacc
     4201 aatgttcagc gcacgatagg taaccgggaa taagtgggcg tctgaataat ttccttctcc
     4261 cattaaaacg caggatggtt tttgccattt ccaaatgggt tctcctccca tattcttctg
     4321 accacgtggt tcgaaattca tataattttt tccggtaagt aaggtcgcaa gatcatcgtg
     4381 caaccaaccg ccaccattaa agcgcgtatc tacaatcagt gcctttttag tatgcatcac
     4441 cccaagcgct ttgtcgtaca catctctgaa acttggacta tccattccgc gaacatgaac
     4501 atatccaatt gttcctcccg ataatttctc cacttctttt tcgcattgct taatgtagcg
     4561 atgataagca agatcatttt cctctccctg cgaaatcgct ttaatggttt ctgtccagga
     4621 attttgtccg gaacgaaacc taaccacaac gggtttgcca accttacgat ttagcaaggc
     4681 ataaatgttc gttgcctgac caaccggctc tccatcgatt tcagtgatca gtgttcccgg
     4741 aacaatcttt ccgctggcaa gtaaaggact gcgtggaaga atttctgcaa ttcgataacc
     4801 ttcacccagg tattcttcgt cgtaaaaaca tcctaacgac gatgtctgat cgccgttggg
     4861 atcgtaatac ctgtaggttg ctcccgtatg tgatgcgtta atttctccca acaattctgc
     4921 aagtaattct gcaaaatcat atccgttatt gatgtggtac aaatgttttc cgtattcctt
     4981 tttgtaaaaa tcccagtcga ctccatgcaa atctgccaga taaaatttct ttttggtttg
     5041 tctccacgcg tgttcaaaca tatatgcacg ctcatcggca ggacggtgtt caaattggct
     5101 ttctacagta ataggagtta ttgccccatt gctgatttca actttcatca gctgaccatt
     5161 cttcgtgaag aaaataaatt tctcctcttt atcaaatgtc attgcggaag gtgagccacc
     5221 aactttagca agcagtttcg tttctccgtc tttaaacttg gttgtccaga tatcgaatcc
     5281 cttttcgtaa ctcgtcatgt aaaaaagttg agtcgcatct ttattcagca gataatcgcc
     5341 aagatgagaa gagtgaaccg ttaaccgagt gcgacgatcc tgcaaacctt cttcatcgat
     5401 taccaggggt tttacttctt cctttttatc cttgctttcc gttgccttct tgtctttcgc
     5461 ggaagtttca gcggattttt cctcgggctt ttgtttttcc ttttctaatt ccgattcgta
     5521 ttccgattct gacttcgatg ctttgtattt tctccatgct tcgcgtgtca gaaaaatggc
     5581 ttccacatca ccttctgcac cccaactgcc atgagagcgg taaccaaact tatcggttgt
     5641 ccagcacacc atttctccac ccatggcaaa ctttggcgaa ccgtttccat agccgctctt
     5701 gcttaagttg atcggttttt ttcctccctg tgcgcttacc aatcctacat tgctcaccca
     5761 gcgatcgtat tcaaaaaact caaccaataa ccatttgctg tcgggcgacc aggtgtaata
     5821 ctgatcgcca tcggagtagg aataattata gctgccttcc atcgcagtaa caattttgcc
     5881 ggtggtaaga tccattaccc tgagtgtagt gcgttcttcc agaaaggcta ccgattttcc
     5941 gtcgggagaa tacgcaggct ggaattcttc ttccggacgc gtgctcaatg ctttttcggt
     6001 gatctgtgtg caattgtaaa aatatttttc accggaattg gtaatactgc tttcataaat
     6061 atcccagctc tgtgcacgtt ctgcggcata taccaatttt cttccgtcgg gagaaaaaga
     6121 aacactgcgt tcttgttgcg gggtattggt gatgcggcgc gttgtagaat gtgccacagc
     6181 ggatacataa atttctccgc ggtaaacaaa tgcaatttcc ttaccgttgg gtgaaaggct
     6241 gtattcagaa acattcccgt ttaccgatat tggagaaaca ttgttttcgg aatgatcggc
     6301 cagaaccgat atatttactt tttttgaatt tccattctca aacaaataaa tttccccatc
     6361 gtatccataa caaagtacgc cattttgaga agaacttaaa aagcgaacag gatgtttatc
     6421 gtgttgggtt aattgttgct gatgtgattc tcctccgatg atccccttcc agatattgaa
     6481 tgaaccggat ttttcgctga ggaaataata ttccttttcg cctgcccaaa caggatttcg
     6541 atcctcgcct ttccaggagg cctgacgggt aaatgttgaa gttgaaagat cgtacaatac
     6601 gatatcgcgg gttacactgc tggtatggtg ttttctccag ggatcttcat acccttttcg
     6661 gtcgtgcatt aaaattctct ttccatctcc cgaaaattgc aaggcttcag ccggaacagt
     6721 aagtttcatt ttttccttac cgccgtttac tgcaaccgca tacaattcat gcaatacttt
     6781 agagggaaac attgcattgc gataatcatc caaacgcgca gaaccgaacc agactgcggt
     6841 tccttcggga ttgaatgtat agggaaaatc ccctgcagaa tggaaggtga gccgtttagg
     6901 aaatccgcct tcggaagaca tcacataaac atcaaaattt ccgaagcgat tggatgcaaa
     6961 tgcgatttgt tttccgtcgg gactccatac cggcataaaa tcgtaagcag aatgagaagt
     7021 cagctgcatt gctcttccgc cctgagaaga aaccttccag agatcgcctt tgcaggaaaa
     7081 aacaatttct tttccatcgg gggatataac aggataacga aaccagttgg cataaacaga
     7141 tggcgctaat aaaaacagca tggccgttag aaaaaacagt cttctcatag tggtagtgga
     7201 tttcctttaa atgtaaccaa aagcaaaacg ccccctgaaa aggaggcgaa aaaattatga
     7261 acgaatctat gattccttat gccggattag tgcggggagc cccgccattc ggacgttttt
     7321 tcttgccctt gaacggacgt tttttcttag ccgaaggatc taccggtgcg ttttcgtcga
     7381 tacccggacc caattcttca gggattggca tccgttttac tttcattgcg atcaattgct
     7441 caatgcgctg gaaacggcga atatcttttg catcgataaa cgtaatggca gttccggtac
     7501 tcgaagcgcg ggcagtacgt ccaatcctgt gcacataatc ttccgcatcc ggtggagcat
     7561 cgtagtttac aacaagatca atgccttcaa cgtcgattcc cctggagaga acatccgttc
     7621 caatcaggta acgcaatttc cgattgcgaa aatcgcgcat gatctcttcc cgttcgtttt
     7681 gttcgagatc agagtgaaaa gactgacaag caaatccctt cttacgcaac gaatgaaaca
     7741 attgctttac aagttccttg cgcgatgaaa atattattcc gctttggtat ttctcttctg
     7801 aaagaatacg ttcgagcata ggaattttct gatcttcctt caggagataa acctgctgat
     7861 cgatccctgc tgccggcttg gcaatggcca cactaacttc tttcgggtct ttaagaatgg
     7921 attgcgcaag ttggcgtatg cgaggcgaca tggttgcaga aaacatcagg ttctgccgct
     7981 cttttggtaa ataggaaatg atgcgcatga tgtcttcgta aaaccccatg tccaacatac
     8041 ggtcggcttc atcaagaatc agaaatttta cctgatccag tttcagcatt ccagagctta
     8101 aaaacgaaag taatctgccc ggtgtcgcta caattatatc tactccgctg tcgaaagcct
     8161 tgcgctgacg atcgaatact tctcctactc cacccccata aacagcaatg gaactttgac
     8221 tgacaaagta ggaaagcccc tcgatctgtt gatcgatctg catagcaagt tcgcgtgttg
     8281 gagcgagtat aagcgcgcgg gtccccgagc tgttgctttc aaacaattta ttcatcacag
     8341 ggagtacaaa cgcagcggtt ttaccggttc ctgtttgtgc gcaggcaatg agatcgtgac
     8401 ctcctaatgc tataggaata gcttgttgct gaatgggtgt tgggttgcga atgttcatcg
     8461 catcaagggc gtcgagcaat tcgtcgacca gtcctaaatc ataaaaatcc aaagtacatg
     8521 tattagtaaa ccctgtccgt ccggaacagg aagctgcaag ttactcaaaa tcccccacaa
     8581 gcccaaggcg acctttccta ttggaatcct gccgttcaat aaacaagtac aacacatggt
     8641 cattttcatg taaagctgaa aaaaaacaac ttaccttgtg gctctattct ctctaatgtt
     8701 acgaaacttc ctcctcgcat tattgctttg tgtttcctta ccaggatggg cacaacaact
     8761 ggtcgttgac aatgccggta attacagcaa cccgaattat ttaactcaaa acattcttcg
     8821 cggtggcagt gtggcaatca gcaacgtttc attctccgga attagcagtc agcaaattgg
     8881 ttatttcacc aatggatctt ccgcaaccgg actttcatcc ggtatgatcc tttccacagg
     8941 aaaagccatg aatgcagcag gcaccaacga cagcaccaac acatccggtg gattaggctc
     9001 aagcggttca acagatcctg atgtgatgag ccttgccaac ggacaagcga tttttgatgc
     9061 gctcatttac gagttcgatg tgcgtcccga tggcaatgag ttaacgctcg aatacattct
     9121 tgcatctgaa gaatacacgg aatacgtttg ctcccaggaa gcagatattt ttgccattat
     9181 catcagcggc ccaggtatta ccggaacatt cagcaataac ggaaagctca tctcgcttat
     9241 tccaacatcg agcactattc cggttggcat caattcaatt aacaacggaa ctcccggtac
     9301 attcggagct cccggcggat gttcgaattc ctttttctct ccatactacc tcaacaacac
     9361 acaaaattta tttgaatacg atggattcac aactgttctt caagcgaaag cagatgtgga
     9421 atgcaatcag ttgtatcata ttcgaattgt tctcgctgat attggcaata acggttttga
     9481 taccggactc tttctgaaag aacgcgggct gtattccaac agcaccggta acttcgatga
     9541 aggcgccttt ctcgactcag taattgttga agggtgtaaa ccattttata tggttatata
     9601 caatccaatc ggttttacag gaggacaaac atttcaggtt aacatcggtg gtactgccat
     9661 caatggcacc gactatgatt ttgttccttc tacttataca atgaattccg gcgacagtct
     9721 tttaacaatc gtatttaatc cggttgctga tggtgtagtt gaaggatttg aatcggtgat
     9781 cgtatcctac ttctacctta acccctgtgg agataccgtt tttgtaaaca aaacctttta
     9841 catccgcgat gaaagtccgc ttgttgttac cggccttccg caaaacacca gcatctgcaa
     9901 tggtcagtcg gtattattga atgtaaatgt tagtggcggt tttccttcgt attctatttc
     9961 ctggaacgga atgctgcaaa acaattttaa tgtgacaccg atacaagaca tcacttatgt
    10021 cgcagaggtt tacgatttag caggttgtta ttggaccggt agttttactg tcgatcataa
    10081 tccgagtcca atcgtaaatg ccggtccgga tattacgatt tgttccggtc agccagagca
    10141 actcggatta caaatagaag gtggtccggg cgcaacctac aactggatgc cggccagtca
    10201 gctgaattca ggaaaccttc cttatcccac aatgaatccg caaaccacac aaacatttac
    10261 agtaggcgtt gtaactgcag caggttgcac aggaaccgac cagcttgttg ttactgtact
    10321 gccatcacct acagtaaatg caggtccgga tcaaagcatc acttatttgc aaaccaccac
    10381 cacacttatt ggttcaggtg gaggagcgcc ctcttggtat ccacctgatg gattgagttg
    10441 cacgaattgt ttcactccgg aagcctcgcc ttataccact accacttata ctttgtatat
    10501 cacctccgga aacggttgcg tatcgagtca ggaagtagtg gttgaagtgg agattccgat
    10561 ggacgtattt gttcccactg cattttctcc gaacaacgat ggggtgaatg atttgcttat
    10621 tgtaaggagt tataccatcg aaagtatgaa tctgaaaatt tacgacacct ggggacagtt
    10681 actttatgag aatacgcatc ccgattgggg atgggacggc acggtgaatg gtgtacctgc
    10741 caatattggc ttatatgtat atacgttgga agtagtattt attcagggaa aaggaacagc
    10801 aacattttcc ggtgagttca atttagtgcg atgagaaaag tttatttatc tgtagctctc
    10861 tggatattct gtttgggaat ttcccgtgca caggatgcac attttaccca attcgaaaac
    10921 tctccttttt tcaccaatcc tgccgcagcg ggaatgattg agcaaagtga ttttcgcgga
    10981 tctctgatca cccgaaatca atggagaaaa ctggtgcgtc caaccaatca aacactcgta
    11041 tcggcagatg caccattatt acgttaccag ggaggatcgc ggagtaaaag caatctcggt
    11101 attggattaa gctatggttt tgcaaccatg ggtgattccc gcacccgcat gaccaatctc
    11161 gccctggcct taagcggaat gacaactgtt ggaacacgca aataccttgg cattggcgta
    11221 tcggcaggta aaggaggcat gcgcagcgat ttaaccaatg caacctggga tgcacaatac
    11281 aacggataca aactggacga atcacttccg actggagaag cctatgaagg aagattgaaa
    11341 gcgcgttttt ttgatctttc agccggacta aattttcttt cggtgaatgg gcgcacagga
    11401 gcaaccacga attttggaat agcttacctg catgccaaca atcccaaact aaaaaacgaa
    11461 cccctgctta acggaaaaat cgatccccgc attgttgcgc atttccgttc ggagatacaa
    11521 ctggatctga aaaataccat gcccgtattt atcattccgc gcgccctggt tggaatgcaa
    11581 ggagtacata tggagattca ggcaggagca accgttttct ggtcggcaga aggcacttct
    11641 cagataacag gataccgtca caaaaccggt ttcgaggcag gaggcatgta ccgacatggt
    11701 gatgctgttg gattacttgc aggatggaaa acagaatcgt accgtatcgg gctttcttac
    11761 gacatcagta tttccgctta cggaaaagca ctgaatcacc gtggaggaac tgaaatcagt
    11821 ttcatttatt acgcagtaaa caaagagatg cgttcgcgca aggccattta tgattgatcg
    11881 gtatcagtca ttggattgaa aaagaatctg agcagtatat aaaaagaaaa aggggaccat
    11941 cggtcccctt tctcattgtc tttaagacgg aattacttca cgttcttaga aacgatctta
    12001 gcaaggtcga acatgctgat ctgagcctta ccagcgaaga gagccttcaa cttagcatca
    12061 gcattgatca tacgcttgtt cttcttgtcc tgaaggccgt tcttcttgat gtaatcccag
    12121 atctttttaa cgatctcagt acgtggcact ggcttgttac cgattacgtc agcaagaact
    12181 gtgcttggag taagcggggc catgaaagcc gcgtttggtt tacgagctgt cttcttctta
    12241 ggagcagctt tcttagcagc aaccttcttc ttaggagcag ctttcttagc agcagctttc
    12301 ttaggagcag cttttttagc aacagctttc ttagctggct tctttgcagc agccttctta
    12361 gcagctggtt tcttagcagc cttcttaggg gcagctttct tggcagcagc cttcttagga
    12421 gcggcctttt tctttggtgc agttttcttt gccatgattg tttggatttg tatgcgcaaa
    12481 ttgaagatct ttattgacat ggcaatgggg taagtaaaaa tatttttaac agggtaatca
    12541 acaatttccc catagggaaa aacactactt tattcaccat ttcccctcgg aaaaacccga
    12601 tttttacagt gattccccaa tgaaaactca cttttacccc tatgtgaaaa acccttctac
    12661 caaaatgccc gcccttaggc gcgcacccta gcggggacgg tttattacct taatggaagt
    12721 cacaggatgt atctcccagg cggtcgctcg ccagttattt tcacgaagcg ggcgcgtgtt
    12781 ttcgctcgtg ttaagatgct cttcatccaa aaacaaccat ccttccacct ccacccatcg
    12841 tcccaaaaac tgatctctca gtccgcgtgt tgtccagttt tctccccgca acttcataat
    12901 ggatcgcatt ggaggggtga cttcagcaat aaaacgtttg ctcttgacct cgttcatagg
    12961 atccaaaacc agatcgatat gcgtatcccg atcatccact tcctttgcac cgcagttaca
    13021 tgtctccttt ccgccgggtt tcacatcaat tacatacgct cttacccgtg cagccttttt
    13081 ttcattccac cgcgcctcat cattgcccgg caacaacaat gcgttcatgg tcgccgacgt
    13141 gtcaaaatcc tgtgcgtgcg gaaatgagat tcggttcttc aaacggttca actcacgaat
    13201 gcgttctgtt ttcgcagatc cctccggcgg acattcacgt gtccaattga gggtgtcggg
    13261 tctttccggt gattgtactg tttctttttt cctgctgctt gcaggtaggg caaagaccaa
    13321 aacgattccg gttattagca atcccagaaa aaacgacaaa agcacaaaac gtttcataag
    13381 gccaatatac caatcctctg ccgaaataag gattttgtat atcttgcctg tatgcgccgc
    13441 agcagtatac ttcgtcaatt actcatcaac acccttgttc cggtggtatt ggttctgctt
    13501 gccgttggag taatcagtta ccgcatcaac cggcagcaac tggaggaaag caataggcgc
    13561 ctgagagaac aaattgttgc gcaaacaaaa tccatactga gcattcacga cgaagcgctc
    13621 catattctgg aagaggatct taacgatcgg atcgaaatgc tatctgatcg tttactgact
    13681 gagtattttg caaagccgga ctccctgcac acggcagatt tgtttcgtat ttctgcagaa
    13741 atcggaatgg atacagccat tgagcattta tatgttatta atgactcctc agtaattacc
    13801 aataccactt ttgcaaaaga cctcggactt gatttctcca aaatcgacac ttcgtttgtt
    13861 aattcttttg ccaagatgcg caaggaagga aatctcgtta tcgaccgctt tggcgaggaa
    13921 atgattacag gaaaaataaa gaagtactcc tttttaccca cccccgacgg caggaacatt
    13981 attgagatgg ggatttattc tgcaatggca gatgggttgc gggacaaggc atttaagaat
    14041 gtgagcgaaa tcagcgcgaa cttccccgag atacgcaaca tcgcttcctt tgcaacggtg
    14101 gccaatgtaa aaaacgttca tcttccggat catcattatg agcattacca gcgagcaatg
    14161 aaagaaaaag taccggtgag ggtcagcgaa agtgaagatg gcatcgatca ttattacgac
    14221 tatttctttc tcgacatcca gtcttcctcc atctacactg gttatcttct gctgatcgaa
    14281 tcggataact cccgagaaaa agcattgata agagccgaag tcattcgctt cagtgtgttg
    14341 tttcttgtaa ccatctcact gcttagtctg gtaatctact accggtcaag gtccatcgct
    14401 ctacccatac gcgtgcttac cgacaaggcc cgggtaattg caggaggaaa actggatgag
    14461 cgtgttccta ttaaggggaa caatgagatc acagatttgt cgcagagttt caataccatg
    14521 atcgacgatc tggaaggcat gtatgtgaat ctggagaaaa aggttaagga acgcacccac
    14581 gaactcaatc accaaaaaga attggtagaa gaaaaaaaca aggagatcac cgattccatc
    14641 aactatgcgc gaagaattca gtatgcactt cttgcacacg atgatctgct aaaggaaaac
    14701 ctccctgagc attttgtact gtttatgcct aaagatattg taagcggaga tttttattgg
    14761 gcttgtctgc accccagggg aaactccaat gatttctatc tggccgtttg cgacagcaca
    14821 ggacatggcg tgcccggagc ctttatgtcc ttgctcaata tttcattcct taacgaagcc
    14881 atcaacgaaa aaacgatcga agaacccggt aaaatcctcg atcatacccg acgcagactc
    14941 attgagaata tttcgcaaca ggggcaaaaa gatggcatgg atggaatact ggttcgtttc
    15001 tccgaaggaa aaataagttt cgcagcagcc aacaatcctc cgctaatcat tcggaacgga
    15061 gaagaaattg aattagcagc agacaaaatg ccaatcgggc aatccgatcg aatcgatcca
    15121 tttaccacag ttatcccgga tctgcaaaaa ggagatatgt tttatctctt caccgacgga
    15181 tatccggatc aatttggcgg accaaaagga aaaaaattca agtaccgtca gctgtatgac
    15241 ctcctgaaag aaatcgccca cctctccatg accgaacaac gcacaattct agaaagcacc
    15301 atcgaaaact ggaaaggaga cttggaacag gttgatgata tttgtgtgat tggaatacgc
    15361 gtttagcgtg ttgcaatcac actaaatatc agaatatcct gaccgacgat aggagcgtca
    15421 gtatagatgc aatatttgtg tgattggaat acgcgtttag cgtgttgcaa tcacactaaa
    15481 tatcagaata tcctgacact gcatttcatt ccggtcagga tgtgccactc accgacgata
    15541 ggcctgcccc gaccgatagc gtcggggagc gtcagtattg atgatatttg tattattact
    15601 cttgtttgac aaaacattat gattgactat tccgaagaaa gtttaaaaac tcttgatcgt
    15661 ttttatcagg ccttgcaacc ggatattgat cgacttgaat ctgcacacgc atcaaaaatg
    15721 caatgcaaaa aaggttgcca tgcctgttgt atcgacgatc tggttgtatt tggggtagag
    15781 gcggctaata ttgttaagca ccattctgca ttagttcaaa acgaaaagcc tcacccgaaa
    15841 ggtaaatgcg catttctaaa taatgaaggt gcatgccgaa tctatgatgt tcgtcctttt
    15901 gtttgccgta ctcagggatt gcccttaaaa atcatcaatc aaaaaggaaa cgagttgcgc
    15961 gatgtttgtc ctttgaacct tgaaggagtt aacctgaata aaatccctga gaaacagtta
    16021 ctgcatacca atccctatga agagtttctt gcccgattac aacttgcgat tgataaggga
    16081 cagatggagc gtatcaaact ccgatcactt ttcgggaaaa aataacccgg tatattcaat
    16141 actaccgggt ttcgtgtgag gttcgtttat ttaaaactcc aatcgtgttt gtcgagatcg
    16201 gttagacagc ctttcagcaa atcaatactg ccgcgaatat catccttatc gctcatctca
    16261 atcacctgat ggacatgtcg gcagggaatg gaaatacaac cggagattgc tcccccgctg
    16321 tcattcgctc tatgcctgca gtatctgttc ccccggcagg aagaatttct ggttgccatt
    16381 ttattttgtg tttatcggct gttttcttca tgaattccac catgcggtaa tcgctgatta
    16441 cgcttgaatc cataatctta atggcggttc ctttcccaag ctcggtgatc atctcatgcg
    16501 ccttcgcccc cggtacatcg taggcaatgg tgatatccag tccgaatcca aaatcaggtt
    16561 gaatagcaag cgcagcaaca ctggctccac gaatacctac ttcctcctga accgtaaata
    16621 caccataaac atcataggca ggatttttta attcgcgtaa agtctccagc aaaataaaaa
    16681 cagaaagacg gttatcgagt gacttgcagt ttacacaatt tcccatttca ataagctcac
    16741 gctcccgggt aaccacatcg ccaacagaga tgtacttctc tacttctttt ttggtcattc
    16801 ccatatcgat aaaataatcg gtaagaggaa ccaccttctc acgctcggat gcgctcatta
    16861 cgtgaatagg cttgcatccc attaccccaa tgagatcctt ttttccgtgt acgattacgc
    16921 gctgagcggt aagtgtttta ggatcaaatc ctccaagcgg aacaaaacgc agaaatcctt
    16981 tttcatcaat atgattcacc ataaatccga tctcatccat atgcgcagcg atcatcactt
    17041 tacgatcact tttctttccc ttcttaacta cggtgatatt tcccatgttg tcgatatcga
    17101 tggaatcaac atggcctttg acttgtttca tgacgagttc gcgaaccaat tgttcacgac
    17161 cgggagcgcc cggtacttcg cagatctgct taagtaattt tacattgata ctcatatcgc
    17221 gtaggacttt tcccaaatat atgcggaata aatccctgta caactcctgg aggtaaaatt
    17281 ccgatgaacg atgtttgcat ccgcatgaat caccagcttt catcctcgta ttattctgcc
    17341 tgtccctacc ggagtcaggt tgcatgatcc cttttcattc agaaccaacc aaccccgtcc
    17401 agcgtgtcgg ggttttttgc ttttaaacca aacccatgaa acagaattac agtaattaca
    17461 acaaagaaga ccaggaagta tggtcaatct tatttagccg acaaattgca caactggagg
    17521 agcatgcatg tactgacttt cgtttaggac tacatcgctt gcgtagcgaa ttaaatgcgg
    17581 atgctattcc cgattttgat cgtctgaata ccttgttgta taaacttagc ggatggagta
    17641 ttgaagttgt tcccggacta atcagtgtaa atgatttttt catgctgctt tcccaacgaa
    17701 aattcccctc ttccacctgg ctgaggaaga aagatcaaat ccattacctc gaagagcctg
    17761 atatgtttca cgatgttttc ggacatattc ccatgctggc gctaaaagag t
//
LOCUS       DAOMYG010000012        16942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1170_length_16942_cov_11.071120, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000012 DAOMYG010000000
VERSION     DAOMYG010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 16942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16942
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1170_length_16942_cov_11.071120"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PK610_00920"
     CDS             complement(<1..707)
                     /locus_tag="PK610_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HRJ34514.1"
                     /translation="MRIGIDIMGGDFAPEATTCGAIIARKELPADVRLVLYGDREQIF
                     SILTREGEDVAQYDIVHTTEVINMNDHPTRALPQKPDSSISVGFRHLKTGEIDAFASA
                     GNTGAMLVGSIFSVNNCPGVIRPCITSILPKMEGGFNIILDVGSNADCKPDVLYQFAV
                     LGSLYIEHVYGVKNPRVGLLNIGEEEEKGNLLTQAAHQLMKGSSDFNFVGNIEGRDLF
                     RDKADVVVCDGFTGNVV"
     gene            complement(772..963)
                     /gene="rpmF"
                     /locus_tag="PK610_00925"
     CDS             complement(772..963)
                     /gene="rpmF"
                     /locus_tag="PK610_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRJ34515.1"
                     /translation="MPNPKRKHSKTRRDKRRTHYKATAGNIAMCPTTGEPHLFHRAYW
                     HEGKMYYKGKVVIDSTATA"
     gene            complement(1008..1523)
                     /locus_tag="PK610_00930"
     CDS             complement(1008..1523)
                     /locus_tag="PK610_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HRJ34516.1"
                     /translation="MKTLDQYIVPFSGLSVGRHEFTLEVKDEFFGCFEASEISKGDLE
                     VKVTLEKSAAMLEFQFRFAGKVRVLCDLCMEEFDMPLEAEEKLIAKFGDEEFENTDDI
                     VVLPHAEHQIQLAQYIYEFIHLSLPARRVHPEGECDPEMIRKLDEFGSREPGDDETDP
                     RWDMLKNIRLN"
     gene            complement(1611..2687)
                     /gene="pdxA"
                     /locus_tag="PK610_00935"
     CDS             complement(1611..2687)
                     /gene="pdxA"
                     /locus_tag="PK610_00935"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRJ34517.1"
                     /translation="MSQSHNDHKVRVGISVGDINGVGMEIIIRTLADNRILQDIVPVV
                     YGNSKLASYYKKALNLPEFNFHSIQNASDAVRNKANLVNCWNDEVTFTPGEANSTGGK
                     YAFISLEAAVKDLAGGKLDVLVTAPINKEVMQSDQFRFPGHTEYLASIAGVDEALMMM
                     VAGDLRVGVLTGHIPLNQVSSQITKDRLQAVVKRMNQSLIRDFGIRRPRIAVLGLNPH
                     AGENGLLGEEDKNIITPAIQSLRNEQLMVFGPYPADGFFGSGNFRNFDGVLAMYHDQG
                     LAPFKSISFNEGVNFTAGLPVVRTSPDHGTAYDLAGKGTADETSFRNAIYLAKDVFLQ
                     RRFHKEITANPLQAQVRSERSERE"
     gene            2820..5489
                     /locus_tag="PK610_00940"
     CDS             2820..5489
                     /locus_tag="PK610_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ34518.1"
                     /translation="MKRLLLLPIFLLLALSGYATHNRAGEITYQWLGGLKYRFIIKIC
                     TNEGSSVADRPELEIWYGDGDRDTVPRLSETPVPSIGSFVGSENIYIAEHTYDGPSTY
                     FIEVLDPNRNQGILNISQSVNVTFCIRSVLVISPFLSTGNNSCVPQDFPCPEIGCVGG
                     RYCFNSAAYDPDGDSLSYELIPCSGGNSNDNGCSPLAGGVYTYPHLVGGGTISLDPLT
                     GTFCWDGPQLQGEYNIAIRINEWRNGFFMGSVVRDIQITILGICNNDPPTLTPITDRC
                     VVAGSNINFSMTASDPNINQSIVIENYGQPFSFTNSPATFSTSGSNPKTGTFNWNTNC
                     SHIRNSAYQIYFIARDNHPTVPMIDVRSGTIKVVPPPVTGLVAAPFANTMVLTWNPNS
                     CANIQGYRIYRKNGHGTPNTSTCCDMNTATQLGYTLIGTTTGVNATTFTDANGLVLGN
                     EYCYVIVAFMLDGAVSCPSNEVCDQLNMDVPVITHVSVGSTNAITGVDTVRWVHPLEL
                     DTVSNFPGPYFYKVYQSIGFGAANTLITTTPQSAQLHLAQKQVLVNNLNTVNNAYSYK
                     VELYQFNTSNSTETLIGPTNNASSIFLTITPADQQLQLRWQLNVPWENTLYEIQRESF
                     PGSGVFNTIATTDTTLFTDTGLVNGLTYCYRIRSTGSYSDPRIPAPLINYSQEVCASP
                     VDLTPPCPPVIVLHNDCEIPLNSLVWNNPNNSCADDVMSYNLYFTPVEGEDFILLATF
                     SSNTDTVFSHILSNGSVAGCYYVTAVDSVQYGNESLPSNVVCGDNCPIYFLPNVFSPN
                     GDGDNDFFKAFPYRFIEKIDLKIFNRWGQLLFLSEDPGFRWDGKSMENGKDVPEGVYF
                     YTCTVYTIRLSGMEEIKLNGFIHLYRENGASSQ"
     gene            5493..6074
                     /locus_tag="PK610_00945"
     CDS             5493..6074
                     /locus_tag="PK610_00945"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRJ34519.1"
                     /translation="MFTGIIEAMGTMQSTETENSNVHFRIKAPFTSELKIDQSVAHNG
                     VCLTVVAIEGDEYLVTAIEETLQKTNLGSLATGSKINLERCMKLGDRLDGHLVQGHVD
                     QTAICDLISDENGSWKFGFRYDEKENVTVSKGSICINGVSLTVVDSSPGYFSVAIIPY
                     TYEHTNFSSLKKGDTVNLEFDIVGKYIARLMQR"
     gene            complement(6075..6566)
                     /locus_tag="PK610_00950"
     CDS             complement(6075..6566)
                     /locus_tag="PK610_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34520.1"
                     /translation="MENFETLDTGGSTGQISSEHRDSFREAGKYMKIYTIVMFVAFGL
                     LVLGLLLFFAAGGSNIYQAAMRELDSQQRAVLEILWPMISIIAIVLPVFMIVFFIAFL
                     RLNRSASALSAASFTGDMNDFRSAMANMRMYWMIVGIGVIAAIVFGIFIAIRMASIIS
                     QYQ"
     gene            complement(6577..6951)
                     /locus_tag="PK610_00955"
     CDS             complement(6577..6951)
                     /locus_tag="PK610_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34521.1"
                     /translation="MENLDQPSNSFPEDILKEIRTMAGWMQFVGIISIIGSVLGVLGN
                     FSGMANGAGANALLGILMQGFAIYIAVILIQKSTHFRNFSANSDGEALLQAMKSTHMY
                     WMLSLVMIAINIAMNILMANMR"
     gene            complement(6999..8744)
                     /gene="aspS"
                     /locus_tag="PK610_00960"
     CDS             complement(6999..8744)
                     /gene="aspS"
                     /locus_tag="PK610_00960"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRJ34522.1"
                     /translation="MFRSHTCGELRLSHQGQTVTLAGWVQRSRDLGGMTFLDLRDRYG
                     ITQLVFNMEINAALCEQARKLGREFVIQAKGKVAERSNKNNTIPTGEIEIVVDELIVL
                     NVSKTPPFTIEDETDGGDDLRMKYRYLDLRRNPVRNNLELRHRMSIVSRNYLDAQGFM
                     EVETPYLIKSTPEGARDFVVPSRMYEGQFYALPQSPQTFKQLLMVSGYDRYYQIVKCF
                     RDEDLRADRQPEFTQIDCEMAFVEQEDILSTFEGLVRHLFKAVKGVDIASLPRMTYAD
                     AMKYYGCDKPDTRFEMRFVELNDLVKNKGFKVFDDAGLVVGINAAGAAEYTRKQLDEL
                     TDFVKRPQIGATGLVYCRYNTDGTLKSSVDKFYSEEELKRWAEAFGAKPGDLILILAG
                     EANKTRKALNELRLEMGTRLGLRDKDTYSCLWVVDFPLLEFNEDANRWFAMHHPFTSP
                     KPEDIALLESDPGSVRANAYDMVVNGVEVGGGSIRIHDRTLQARMFELLGFSPEQAQE
                     QFGFLMNAFEFGAPPHGGIAFGFDRLCSLFGGSESIRDFIAFPKNNSGRDVMIDAPSP
                     IDQAQLDELKIKLNS"
     gene            9060..10379
                     /locus_tag="PK610_00965"
     CDS             9060..10379
                     /locus_tag="PK610_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34523.1"
                     /translation="MQLFIATFFFLTLAPAGVENQNQIALSQFKDGDLQVGKVTATEK
                     RPNDKFIEQAAKTWPVKFVRDGNRVTEIIVNRAGVVEEKFTPDVPGFPAYFVYNSTRL
                     TMTGNVMYYYNWKNAAPEIKYVLALDYDAIRKKTLDEHHKILQEYLTEAFANQTNARG
                     DMAAEQQAKAEKEKAENSIKGKDIKKIEVVWITPSSEAGMQSKIKYGIKATAADGKIF
                     STPGLGGKTPFDDFEITTVGAQPADEFLMVEVDASKIKNDQISLTAKSKFHSISGSAS
                     LPLSYSTPIVLSFRGEPGCGTGMYAHSGRGGGRGKSIVVNAGLSTNGKLVLVEVKDAT
                     SSEVLARLKVQPGVSITIDISGGDGCSGSSSATSRGGDGGAGGSGGNAVVNKSSSVSG
                     LSLNIINKGGRGGKGGKGSAPINNGSEGSSGSNGNISENNQTITLNF"
     gene            10398..11549
                     /locus_tag="PK610_00970"
     CDS             10398..11549
                     /locus_tag="PK610_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34524.1"
                     /translation="MKKTTLAALLVLAVAVNSDAQKLNSLKEKATGKKEASGGSSSEG
                     KRIAKPFAKDFTDSKNISGAYTIAPAMLIGKDNLGRDSYQDLAKWEYVHEESGKIIVQ
                     LNMYFNAEGLKVSMNLVEKYQENFNTRVFKNKDGELWEIDTDVYVLYDNNDKRTTHVF
                     AKDKSKLEAYDLETATAKYDAKRSEINKKESEKMAAKWMGNDTYKAMVGKVGFIKHYS
                     NVSYNRFDILTEKTDVFISTHEIGKPLYYRGYFKAPITAVCGSDCEYNVTFEIDGLKT
                     SRVELSKKSSKWSNNFKKRDADENFFSYAWTLQDDKVWDYAYVYLLYQIKDKFSDGKS
                     FKMKVTIWANRDGVDKEAMAEGTITLVYKKENKDKIDLMMQQFDNFMNE"
     gene            11849..12301
                     /locus_tag="PK610_00975"
     CDS             11849..12301
                     /locus_tag="PK610_00975"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRJ34525.1"
                     /translation="MTLSVHSDEHFMKQALAEAKKAFEEDEVPVGAVIVWNKQIIARA
                     HNLTERLNDFTAHAEMQAFTSATNHIGNKYLNECTLYVTLEPCVMCAGASFWTQIGKI
                     VYGASDEKRGYLKTNPEIIHPKTEMVGGVLAEECGALLTEFFRKKRDK"
     assembly_gap    12546..12645
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12646..13044
                     /locus_tag="PK610_00980"
     CDS             <12646..13044
                     /locus_tag="PK610_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34526.1"
                     /translation="DMNVTASADFSVFKNEMKLTYNVTDQKIEDLRVKVKMAPADIYM
                     TLELARISGKSVDQVVKVYETNRDKGWGVIAKELGIKPGSKEFHELKGNAKNKNEKVK
                     GKGTNNGKGPKAKTGASPAPNNGAAPGKKK"
     gene            13268..13678
                     /locus_tag="PK610_00985"
     CDS             13268..13678
                     /locus_tag="PK610_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009410210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HRJ34527.1"
                     /translation="MYPPELVAPMKKELVDAGFKELLNAGDVDNEMKNTGTTLVVVNS
                     VCGCAAGAARPGVRKAVQNAKIPAHLTTVFAGVDRDAVEQARRYMLPYPPSSPCIALF
                     KDGKLVHFLERHHIEGRSADMIAENLQAAFDEFC"
     gene            13788..>15005
                     /locus_tag="PK610_00990"
     CDS             13788..>15005
                     /locus_tag="PK610_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HRJ34528.1"
                     /translation="MTARTPTPILHMPISTRFAARDFIRTSLGGRLLFFLFLYFELSS
                     TRAGNTCAGADAIIPNGTCSTLRVDGGAGTPALATCTGTIAREEWFSFTATAGVTYTA
                     SYTNSTNNTNPGIYVYSGACGGLTQIACVNATGMGNPVTETLNFTPGSSGTFYIRMVN
                     FGTTNNMDGTLCLTSAAFLSNDEPTGATALSPGTTCSFSTYTNYGATASTCGTIPAPG
                     CAGYIGSDVWFSIVVPATGSLIINSQAGVITDGGMALYTGAPCGTLTLVSCNDNGTGM
                     PTITATNLTPASTVYVRFWEDGNNNNGTFGICVIEPPSNDEPTTATALTPGTSCSFST
                     FTNLNATASTCGTIPAPGCATYAGGDVWFSIVVPATGSLIISSQAGVITDGGMALYTG
                     TPCGTLSLVSCNDN"
     assembly_gap    15006..15105
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15106..>16942
                     /locus_tag="PK610_00995"
     CDS             <15106..>16942
                     /locus_tag="PK610_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34529.1"
                     /translation="TITATNLTPSSTVYVRFWEDGNNNNGTFGICVIQPPANDEPSTA
                     ISLTPGATCSFSTFTNLNATTSTCGTIPAPGCGGYSGGDVWFSFVVPPSGYVTISAQA
                     GVITDGAMAIYSGSPCGILTLISCNDNGTAMPTITSNTLTPGATIYIRFWEDGNNNNG
                     TFGICVTDPCSSSSPVNNDCSTATPLTVYASSCVPLPGTVQCASASADANSCGATADD
                     DDVWYSFVATSTSVNITITSVAGSVTDMYHSLFTGTCGSLGNAVLCSDPNSSTPTGLT
                     IGQTYYIRVYTVSTAPGQTTTFQICVTQMSPCGNPMNNDFCSTPATLSLSASSTFSST
                     TASSYTADNPANINTAFSCGTIQNNSWYQFTATATSHTINISSIAGCTGGIQAQVYAV
                     TRNANNCCTNFSSVSNCYSPGNTNPGTLTANGLTIGNTYVLMIDGWSGANCNYTIAGW
                     SASGVLPIELISFDGMNIGNGNELRWKTATESNNERFDIERSYDGSNFIVIGSVAGSG
                     NSTVPLSYQFFDGNTVQGHTYYRLRQFDFNGENSTSQTIVVFPKENNEAFIYPNPASD
                     KLNIDVYNEFNTRITVRIIDLAGHVFEETFFAEEGVNHFNSSHFSS"
BASE COUNT         4885 a         3867 c         3627 g         4363 t
ORIGIN      
        1 aaaacaacat taccggtaaa gccatcgcaa accaccacat ctgccttatc gcgaaacaga
       61 tcacgtcctt cgatgttgcc aacaaaatta aaatcggaag atcctttcat gagctgatgg
      121 gcagcctgtg taaggagatt gcctttttct tcttcttcac cgatattaag taatccaacg
      181 cgaggatttt ttacaccgta aacatgttcg atataaagcg atcccaaaac agcaaactga
      241 tacaatacat ccggtttgca gtcggcattg gagcctacat caagaattat attaaaaccg
      301 ccttccattt tgggaaggat ggatgtgata caagggcgaa ttactcccgg acaattattt
      361 acactgaaaa tggagcccac cagcattgca ccggtatttc cggcagaagc aaaagcgtcg
      421 atttcgcctg ttttcaggtg acgaaatcca acagaaatac ttgaatcggg tttttgcgga
      481 agcgcgcgcg tggggtgatc gttcatgttg atcacttcgg tggtgtgcac aatatcgtat
      541 tgtgccacgt cttcaccctc tcgcgtcagg atgctaaaaa tctgctcgcg gtcgccatac
      601 aggaccagcc tgacatctgc ggggagttcc tttctggcga tgattgcgcc gcacgtggtt
      661 gcttctggag cgaagtcacc acccattatg tcaataccta tcctcattcc ggtgtgtttg
      721 gaatcggaca aatgtataaa agattcggaa cgcccgtaag gggattcaac atcaggccgt
      781 agccgtgctg tcgatcacca ctttaccttt gtaatacatc ttcccctcgt gccagtaagc
      841 acggtgaaaa aggtgtggtt ctcctgttgt ggggcacatt gcgatatttc ctgcagtggc
      901 cttatagtgc gttcttctct tgtccctgcg ggtcttcgag tgtttgcgtt tgggatttgg
      961 catggctcaa acgtgttttt tattgtgaca actaacctaa ctacttttta attgaggcgg
     1021 atgtttttta gcatatccca tcgcggatcg gtttcatcat cgcccggttc gcgggatccg
     1081 aattcatcga gcttgcgaat catttcagga tcacactcgc cttcaggatg aaccctgcgg
     1141 gcgggtaacg acaagtggat gaactcatag atatactgcg ccaactgaat ctgatgttct
     1201 gcatggggca ggacaacaat atcatcagta ttctcaaatt cttcatcgcc aaacttggca
     1261 atgagtttct cttctgcttc aagcggcata tcaaactcct ccatacagag atcgcacaat
     1321 acacgcactt taccggcaaa gcgaaactga aattcgagca tggcagcaga tttctcgagt
     1381 gtgactttca cctccaggtc tcccttgctg atttcggatg cttcaaaaca accaaagaac
     1441 tcatccttta cctccagcgt gaattcgtgc cgaccgacac tcagcccgga aaacgggact
     1501 atgtattgat ctagcgtctt caccttttta ggattaaaag gacggcaaag gtaacaaaaa
     1561 agaacacccc aaaaacaaaa accgtaaaaa accggcgaaa gggctgaggc ttactcccgc
     1621 tcagagcgtt cggacctcac ctgtgcctgt aaagggttgg ccgttatctc tttatgaaac
     1681 ctacgttgaa gaaacacatc cttggccaga taaatagcgt tacggaaaga agtttcatcc
     1741 gcagttccct ttcctgccag atcgtaggca gtgccgtggt cgggagaagt acgcacaacc
     1801 ggtagaccag cagtaaaatt tacaccctca ttaaacgata tggacttaaa aggtgccaat
     1861 ccctgatcgt ggtacatagc taaaacaccg tcgaaattgc ggaagttccc tgaaccgaaa
     1921 aacccatcgg cagggtaagg accaaacacc atgagttgct cattgcgcaa ggactggata
     1981 gcaggagtaa tgatgttttt atcttcctct cccaggagac cattctcgcc agcatgagga
     2041 ttaagtccca aaacagcaat gcgcggacga cgaatcccga agtcgcggat caagctctga
     2101 ttcattcgct ttacaacggc ctgtaagcga tcctttgtga tttgtgaaga gacctgattg
     2161 agtgggatat gcccggtgag cactccaacg cgcaaatcac cggccaccat catcattaat
     2221 gcctcatcaa cacctgcaat tgatgcaagg tattcggtgt ggccagggaa gcgaaactga
     2281 tcactctgca tcacttcctt attgataggg gcagtaacga gtacatcgag ttttccgccg
     2341 gcaaggtctt taactgcggc ttcaagcgaa atgaaggcat acttgccccc ggtggaattt
     2401 gcttctccgg gagtaaatgt aacctcatcg ttccaacagt ttaccagatt cgccttattg
     2461 cgaacagcat cagacgcatt ctgaatacta tgaaagttaa actcgggcag gttaagcgcc
     2521 tttttgtaat acgatgccag tttggaattg ccataaacaa caggcacaat atcctgaagt
     2581 atacggttat cggcgagcgt gcgaataatg atttccattc ccacaccgtt gatgtcgcct
     2641 actgaaattc caacccggac tttatgatcg ttatgtgact gactcattcg tgttgatttt
     2701 gcacaaaggt aagaatcccc cgttagtaca atccctgcaa cctgtacaac ctttcagccg
     2761 tctgattgta ataatttacg tactttgctg ttcttactgt tcattctccc gttttaccaa
     2821 tgaagagact gcttctttta ccgatttttc tccttttggc gctgagtggt tatgccaccc
     2881 acaatcgcgc aggggagatt acgtatcagt ggctgggagg actcaaatac cgttttatca
     2941 ttaaaatctg caccaacgaa ggaagcagtg ttgctgatcg tccggagctg gagatctggt
     3001 atggcgacgg tgaccgtgat actgtaccaa gacttagtga aacacctgtt ccatccattg
     3061 gaagctttgt agggagcgag aatatttata ttgctgagca cacgtacgat ggtcccagta
     3121 cttattttat tgaagtcctc gacccaaacc gaaatcaggg gattttaaat atttctcagt
     3181 cggtaaacgt gactttttgc attcgctcgg ttctggtcat cagtccgttt ttatcaacgg
     3241 gtaacaattc ctgcgtacct caggattttc cttgtccgga aattggttgt gtgggtggac
     3301 gctattgttt caacagcgct gcctacgatc ccgatggaga tagtcttagc tacgagctga
     3361 ttccctgttc aggcggaaac agtaatgata atggttgctc tcctctggca ggaggagtgt
     3421 atacctatcc tcatttggta ggaggaggaa cgatcagtct ggatccgctt accggcacct
     3481 tctgttggga cgggcctcag ttacaaggag agtacaacat tgccatacgc ataaacgaat
     3541 ggcgaaacgg cttctttatg ggaagcgtgg tgcgcgatat acaaattaca attctgggca
     3601 tttgcaacaa tgacccgccc acacttacac ccataaccga ccgttgtgtt gttgcaggaa
     3661 gcaatatcaa tttttcaatg accgcttccg accccaacat caatcaatcc attgtaattg
     3721 aaaattacgg tcagcccttc agttttacca attcaccggc tactttcagt acatccgggt
     3781 ccaacccaaa aaccggaaca ttcaactgga acaccaattg ttcgcatata cgcaactcag
     3841 cctaccagat ttatttcata gcacgcgaca accatccaac agtaccgatg attgacgtgc
     3901 gatccgggac aataaaagtt gttccgccgc cggtgacggg attagttgcg gcaccgtttg
     3961 caaatacaat ggtgcttaca tggaatccca actcctgtgc taacatccaa ggctatcgga
     4021 tctaccgtaa aaacggtcat ggcacaccca acacttcaac ctgttgcgat atgaataccg
     4081 caactcagtt aggttacaca ctgattggaa ccacaacggg agtgaatgca actactttta
     4141 ccgatgcaaa tggtcttgtt ctcggaaatg aatattgtta tgtaatcgtt gccttcatgc
     4201 ttgatggcgc agtgagttgt ccgagcaacg aagtctgtga tcagctaaac atggatgttc
     4261 cggtgatcac acatgttagc gttggaagca ccaatgccat aacaggagta gatactgtgc
     4321 gttgggttca tccccttgaa ctggatacgg taagtaattt ccccggaccc tatttttata
     4381 aagtttacca gagtatcgga ttcggtgctg caaatacact aattaccacc acaccacaat
     4441 ctgcgcaatt acacctcgca caaaaacagg tgttggtcaa taatcttaac acggtaaata
     4501 atgcctatag ctacaaggtc gagttgtatc agttcaacac ttccaacagc acagagacct
     4561 taatcggccc aacgaacaat gcttcttcaa ttttcctcac aataacccct gcagatcagc
     4621 aattgcaatt gcgctggcaa ttgaacgtgc cctgggaaaa tactttgtat gaaatacaac
     4681 gggaatcctt tcccggctcc ggtgtgttta acacaattgc aacaacagat accacgctgt
     4741 ttacggatac cggtttggta aacggactca cttattgcta tcggatccga tccacaggat
     4801 cttattccga tccgcgaata cctgctccgc ttatcaatta ctcgcaggaa gtttgtgcct
     4861 cgcccgtaga tcttactccg ccttgtccgc ctgtaattgt actccacaac gattgcgaaa
     4921 ttcctctgaa tagcctcgtg tggaacaatc ccaacaacag ttgtgcagat gatgtaatga
     4981 gttacaatct ctactttact ccggttgaag gtgaagattt tattctcctg gccactttca
     5041 gcagcaatac agatactgtt ttttcacaca tactcagcaa tggttctgtt gcaggatgtt
     5101 attatgtaac tgcagttgac agtgttcaat acggtaatga aagtttacca agcaatgtgg
     5161 tttgcgggga taactgtccg atctattttc ttcccaatgt gttttcaccg aatggtgatg
     5221 gcgacaatga tttcttcaag gccttcccgt accggtttat cgaaaagatc gatttgaaaa
     5281 ttttcaatcg ctggggacaa ctacttttcc tttctgaaga tcccggtttc cgctgggacg
     5341 gaaagagtat ggaaaacggg aaggatgtcc ctgaaggcgt ctatttttac acctgcaccg
     5401 tttataccat tcgtctgagt ggcatggagg aaatcaagct gaatggcttt attcacctct
     5461 acagagaaaa cggagcctcc agtcagtaaa ccatgttcac aggaattatc gaagcgatgg
     5521 ggacgatgca atccaccgag accgaaaaca gtaatgttca ttttcgcatc aaggcgccct
     5581 ttacctccga attgaagatc gatcagagtg ttgcacacaa tggtgtttgt cttactgtag
     5641 tagctattga aggggatgaa taccttgtaa cggccattga agaaacgctg caaaaaacca
     5701 atcttggttc tcttgccaca ggaagtaaaa tcaaccttga gcgctgcatg aagctgggag
     5761 atcgtttaga cggacatctg gtacagggac atgtcgatca aactgcaatt tgtgatttga
     5821 taagcgatga aaacggcagc tggaaattcg gattccgtta cgatgaaaaa gagaatgtaa
     5881 ccgtaagtaa agggtccatc tgcatcaatg gcgtaagtct aacggtagtg gattccagtc
     5941 cgggatattt ttcggttgcc attattccct acacctacga gcataccaat tttagtagcc
     6001 ttaaaaaagg agatactgtc aatttagaat ttgacattgt gggtaaatac attgcccgcc
     6061 ttatgcagcg ttgattattg gtattgggaa atgatggatg ccattcggat agcgataaat
     6121 attccgaata caattgcagc aattacacca atacctacaa tcatccaata catacgcata
     6181 ttggccattg ccgaacgaaa atcgttcatg tctccggtaa aggatgcggc actcaaagca
     6241 gaggccgaac ggttcaggcg cagaaatgcg ataaaaaaca caatcataaa aaccggcaat
     6301 actattgcta taatagaaat cataggccaa agaatctcca atactgcacg ttgctgtgaa
     6361 tcaagttctc gcatagcggc ctgataaata tttgatccgc ctgcagcaaa aaacaagagt
     6421 aatcccagaa ctaacaaacc aaacgcaaca aacattacga tggtgtagat cttcatgtat
     6481 tttccggctt cacgaaacga atcgcggtgc tcggaagaaa tttgtccggt gcttccgcct
     6541 gtgtctaatg tttcaaaatt ttccatgttc taaagattat ctcatgttag ccatcaaaat
     6601 attcatagca atattaattg caatcatcac cagcgaaagc atccaataca tatgggttga
     6661 tttcatcgcc tgcaataatg cttctccgtc tgaatttgca gaaaaattgc gaaaatgagt
     6721 ggatttctga atcagaatta ctgcgatata aattgcaaat ccctgcatta aaattcccag
     6781 taaagcgtta gctccggcgc cattggccat ccctgaaaag ttcccaagaa cccccaggac
     6841 acttccaata atggaaatta ttccgacaaa ctgcatccag cctgccattg tacgtatttc
     6901 tttcaggatg tcctccggaa aagaatttga aggttgatca agattttcca tggccacaat
     6961 ttatgcaaaa tttactatcc aacaagtgaa aagaataatc aggaattaag tttaattttt
     7021 aattcgtcca actgcgcctg atcaattggc gagggagcgt caatcattac gtcgcgaccg
     7081 ctattgttct tagggaatgc aatgaaatca cggatcgact cgctaccgcc aaataaggag
     7141 cacaaacggt cgaaaccaaa agcaatcccg ccatgcggag gtgcgccgaa ctcaaacgca
     7201 ttcatgagga aaccgaactg ctcctgtgcc tgttcagggc tgaatccgag cagctcaaac
     7261 atacgggcct gcaaagtcct gtcgtgaata cgtattgatc ctccgcctac ttccacgcca
     7321 ttaacaacca tatcataggc attggctctt acgctgcccg gatcgctttc caacaaggca
     7381 atatcttccg gtttggggga agtaaagggg tgatgcatgg caaaccagcg attggcatct
     7441 tcattgaatt ccaacaatgg aaaatccacc acccataaac aagaataggt gtccttatcg
     7501 cgaagcccta aacgcgtacc catttccaaa cgaagttcgt taagtgcctt acgggttttg
     7561 ttcgcttcac ctgcaagaat caatatgaga tctcccggct tggcaccaaa tgcttctgcc
     7621 catcgcttta actcttcctc gctgtaaaat ttatcgaccg atgatttcaa tgtgccgtca
     7681 gtattatacc tgcaatacac caatcccgta gcaccaatct ggggacgctt tacaaaatct
     7741 gtcagttcat ccaattgttt acgtgtgtat tcggcggctc cggcagcgtt gattcccact
     7801 acaagtccgg catcatcaaa tactttaaag cctttgtttt tcaccagatc gttcaactca
     7861 acaaacctca tttcaaaacg ggtatcgggt ttatcgcaac cataatactt cattgcgtct
     7921 gcgtaagtca ttcttggcag agaagcgatg tctacgccct taactgcctt gaacaaatga
     7981 cgaacgagtc cttcaaacgt tgaaagaata tcttcctgtt ctacgaaagc catttcacag
     8041 tcgatctgcg taaattccgg ctgacgatcg gcgcgcagat cttcatcgcg gaaacatttt
     8101 acgatctggt aataacgatc gtaaccgctt accatgagca attgcttgaa tgtctgcggc
     8161 gactgaggca aagcgtaaaa ctgaccctca tacatcctgc tgggcaccac aaaatcgcgt
     8221 gcaccttcgg gagtagattt aatgagataa ggcgtctcca cctccataaa tccctgcgca
     8281 tcgaggtaat tccgacttac aatcgacatg cgatgacgca attcaaggtt attacgcacc
     8341 ggattacggc gtaaatcaag gtatcggtat ttcatccgga gatcatcgcc cccgtcggtt
     8401 tcatcttcaa tggtaaatgg cggagttttt gaaacattca aaacgatcag ttcatctacc
     8461 acgatctcaa tttcgccggt tggaatagta ttgtttttgt tggagcgctc agccactttc
     8521 ccttttgcct gtatcacaaa ctcacgtccg agtttacgtg cctgttcgca caaagcagca
     8581 ttgatttcca tattgaatac caactgtgtg ataccataac gatcgcgcaa atcgaggaaa
     8641 gtcatgcccc caagatcgcg agagcgttgt acccatcctg cgagggtaac ggtttgacct
     8701 tgatgggaga ggcgcaattc gccacaggtg tgtgatctga acatactctt attaattaat
     8761 gcgcaaaact aaacaatcgg acgacaaccc actaataagc atattggaat agcagaaata
     8821 gtgggcaaga agaaagaaaa accggtcata acgtctcctt cgtcctttca aataagctga
     8881 aaggcggctc atttgagctt tcccgttttg aataaggaat ttcataaaac gtcggtttca
     8941 gttcatgaac ggaatcgaaa aaagtaaact ctccatttat gaaatacgaa cgacggttca
     9001 tgaaatccca acaggtttag tggattagcc ggatgatctt tgtggaaatc atttaaacca
     9061 tgcaactatt cattgccacc ttttttttcc tcacgcttgc acctgccggc gtagaaaacc
     9121 aaaatcaaat tgcgctgtcg cagtttaaag atggcgattt gcaagtagga aaagtaaccg
     9181 caacggaaaa gcgtccgaac gacaaattca tcgaacaggc ggcgaaaaca tggccggtaa
     9241 aatttgtcag ggatggaaac cgtgtaacgg aaatcattgt aaacagagcc ggagtggtag
     9301 aagaaaaatt cacccctgat gttcccggat tcccggccta ttttgtatac aacagtaccc
     9361 gtcttacaat gaccggtaac gtaatgtatt actataactg gaaaaacgct gctcctgaga
     9421 tcaaatatgt tttggcatta gattatgatg ccatcagaaa aaaaacactg gatgagcacc
     9481 acaaaatatt acaggaatat ctgactgaag cgttcgccaa tcaaacaaac gccagaggag
     9541 acatggcagc agaacagcag gcaaaagctg aaaaagaaaa ggccgaaaat tcgataaaag
     9601 gaaaagacat caaaaaaatt gaagtggtgt ggattacacc ctcttcggaa gccgggatgc
     9661 aatcgaaaat aaaatatgga atcaaagcca ctgctgcaga tggaaaaata ttctcaactc
     9721 ccggtctcgg tggaaaaact ccgtttgatg attttgaaat cacaaccgtt ggcgctcaac
     9781 ctgctgatga atttttgatg gtagaagtgg atgcatccaa aatcaaaaac gatcagattt
     9841 cgcttactgc aaaaagcaaa ttccattcaa tatcaggaag tgcttcttta cccctttcgt
     9901 attcaacacc aatcgttctt tccttcagag gcgaacccgg atgtggaacg ggcatgtatg
     9961 cacattcagg cagaggtggt ggacgaggaa aaagcattgt ggttaatgca ggtttatcca
    10021 ctaacggaaa attggtttta gtcgaagtga aagacgctac aagcagcgaa gtgctagcac
    10081 gcttaaaagt acaacccggc gtaagtataa ctattgatat ttccggtggc gacggttgtt
    10141 caggaagcag ctcagcaacc tctcgaggcg gcgatggtgg agcgggtgga tcaggaggca
    10201 atgccgttgt gaataaatca tcttctgtta gcggcttatc cctaaatatc atcaacaaag
    10261 gcggaagagg tggaaagggc ggtaaaggat cggcacctat caataatggc agcgaaggaa
    10321 gctccggaag caatggaaac atcagtgaaa acaatcaaac cataactctg aatttttaac
    10381 ccataacaaa aatgaaaatg aaaaagacaa cacttgccgc gttacttgta ttggctgtag
    10441 cagtcaacag cgacgcacaa aagctgaatt cgcttaaaga gaaagcaacc ggaaaaaaag
    10501 aagcttcagg tggatcctcg agtgaaggga agcgtattgc aaaaccattt gcgaaagatt
    10561 ttaccgacag caaaaacatt tcaggggctt atacaattgc acctgcaatg ctcatcggaa
    10621 aagataatct gggtcgcgac agttaccagg atcttgcaaa atgggaatac gttcatgaag
    10681 aaagcggaaa gatcattgta cagctcaaca tgtatttcaa cgcagaaggc ttgaaggtga
    10741 gcatgaatct ggtagagaaa taccaggaaa attttaatac ccgggtattc aaaaacaaag
    10801 atggagagct atgggaaatt gacaccgatg tgtacgtttt gtacgacaac aacgacaaac
    10861 gaactacgca tgtttttgca aaggacaaat cgaagctgga agcatatgat ctcgaaactg
    10921 ccactgcaaa atacgatgct aaacgcagcg agatcaacaa aaaggaatcc gaaaaaatgg
    10981 cagccaagtg gatggggaac gacacttaca aagcgatggt tggcaaggtc ggtttcatca
    11041 agcactactc caacgtaagc tacaaccgtt tcgatattct tactgaaaaa accgatgttt
    11101 tcatttccac tcacgaaatc ggaaaaccat tgtactaccg cggctatttt aaagctccaa
    11161 tcactgcagt ttgtgggtct gattgtgaat acaatgttac tttcgaaatt gacggactga
    11221 aaacctcacg tgttgaactt agcaagaaaa gttcaaaatg gtcgaacaat tttaaaaagc
    11281 gtgacgccga tgaaaacttc ttctcgtatg cgtggacact tcaggatgat aaagtttggg
    11341 attacgctta tgtgtatctt ctgtatcaga tcaaggataa attttccgat ggaaaaagct
    11401 ttaagatgaa agtaaccatt tgggcaaacc gcgatggtgt tgacaaggaa gcaatggctg
    11461 agggaacaat caccttggtt tacaaaaaag aaaacaagga caagatcgac cttatgatgc
    11521 aacagttcga taatttcatg aacgaataat ttttggaaag ctttcgaaaa atccccctga
    11581 cgaactctgg gggatttttt tatttaccaa aagtccggct ttatttttct taataactaa
    11641 caaacaagct caaaatacaa ctcgactgtt tgtgtaacca cgcactccac tggttgcagc
    11701 gacgagtcat aaagcaaatc cggcacgttc aattgaaaga aaaatcctac atgcgataga
    11761 acacccctgg cttcagccag gggttaaagg ggttgaagcg gtagaaatcc tcggactgaa
    11821 gcccgggatt tctgagtaaa ttcgcggcat gactctctcc gtacactccg acgaacattt
    11881 catgaagcag gctcttgccg aagcaaaaaa agcatttgaa gaagatgaag tcccggtagg
    11941 tgctgttata gtctggaaca aacagattat tgcacgagcg cacaacctca ccgagcgact
    12001 gaacgacttc accgcccatg cagaaatgca ggcattcacc tccgctacca atcatatcgg
    12061 taacaaatac ctcaacgaat gcacattata cgtcacgctc gaaccctgtg tgatgtgtgc
    12121 cggagcatcg ttctggactc agatcggaaa aatcgtttat ggtgcctccg atgaaaaacg
    12181 cgggtacctt aaaacgaatc cggaaatcat tcaccctaaa acagaaatgg ttggcggagt
    12241 actcgccgaa gaatgcggtg ctttgctcac agagtttttc agaaagaaga gagataaata
    12301 attctgtaat cgatttggca cactcatttc atctccgaaa taatgcagtt agccgacacc
    12361 ccgtaactct tcgccgttta ccgtttaccg atcacaaaaa ttgtttatct tttggtaacc
    12421 aaactaaacc ccatgaagac gatcttttct ctgacattgc tttttgttgt gcattgtgca
    12481 acagcgcaga ctactgtaaa attcgctgct aattcgggcg atagtgagct tgatctgacc
    12541 ttgagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngatat gaatgtcact
    12661 gcaagcgcag atttctctgt atttaaaaat gaaatgaagc tgacgtataa tgtcaccgac
    12721 cagaaaattg aagacctgcg tgttaaggta aaaatggcgc ctgcagatat ttatatgaca
    12781 cttgagttag ctcgcattag cggtaaaagt gttgatcagg tagtaaaagt ttacgaaacc
    12841 aaccgtgata aaggctgggg tgtgattgcc aaagaactgg gtatcaaacc cggatcgaaa
    12901 gaatttcatg aactaaaggg caatgccaaa aacaaaaatg aaaaagtgaa aggcaaaggt
    12961 accaataatg gaaaaggtcc taaagccaaa acaggagcct cccctgcacc taataacggt
    13021 gctgctccag ggaaaaagaa atgagcccgg gcttcgactc cccaacgctc cccgacgcta
    13081 tcggtcgggg cagggtcggt cggggcaggt acggtgggcg caggtcgctc agccctcgct
    13141 gaagacgcac tgccctcatc catgatgaac ttattcgttt cattgattgt tattgataca
    13201 atgatgttgc tacaatattc ctaattttgc agcagctaaa tttgtttcac ttaaaaacat
    13261 tttccatatg tatccccccg aattggttgc cccaatgaaa aaagagcttg tagatgcagg
    13321 cttcaaggaa ctcctgaacg caggtgatgt cgacaacgaa atgaaaaaca caggcactac
    13381 gttggtcgtg gtaaattccg tttgcggctg tgctgcagga gctgctcgtc ccggtgttcg
    13441 taaggctgtt cagaatgcta aaattcccgc tcaccttact actgtttttg ccggtgttga
    13501 tcgtgatgct gtagaacagg cacgtcgtta tatgctgcct tatccaccat catctccttg
    13561 catcgcattg ttcaaggatg gtaaactggt gcattttctt gagcgccacc acatcgaagg
    13621 acgcagtgca gatatgattg cagaaaacct gcaagccgcg tttgatgaat tttgttgatt
    13681 ttcctattcc ttcttttcag gctccatggt accatggggc cttttttgtc cacgaataaa
    13741 ggtcattcat ccaagtccga caactttgcc tgacaacttg cgtaattttg accgcacgca
    13801 cacctacacc gattttgcac atgcctattt ctacacgatt tgcagcgagg gattttataa
    13861 ggacatcgct tggggggaga cttctttttt ttctatttct ctattttgaa ctatcatcaa
    13921 caagagccgg aaacacctgt gccggggcag atgccataat tccgaatggg acatgttcaa
    13981 ctttaagggt agatgggggt gccggtactc ctgccttggc tacatgcacc ggaacaattg
    14041 caagagaaga atggttttca tttaccgcaa ctgccggagt tacgtatact gccagttata
    14101 ccaatagcac caacaacaca aatccgggga tctacgtgta cagtggtgca tgcggaggat
    14161 tgacccagat tgcttgtgta aacgcaaccg gaatgggaaa cccggtgact gaaaccctca
    14221 actttacacc tggttcatcc ggaacttttt atatccgaat ggtcaatttc ggaaccacca
    14281 acaatatgga cggaaccttg tgtttgactt ctgcagcttt tctatcaaac gacgaaccta
    14341 caggagcaac tgccttaagc ccgggaacca catgttcctt ctctacatat accaactacg
    14401 gtgcaacagc aagtacttgc ggaaccattc ccgctccggg atgtgccgga tatataggta
    14461 gtgatgtgtg gtttagtatt gtagttcctg ccacaggttc gcttattatt aattcccagg
    14521 caggagttat taccgatggc ggaatggcgc tgtatactgg agcgccctgt gggactttaa
    14581 cacttgtaag ttgcaacgat aacggaaccg gaatgccaac gataacggca accaatttaa
    14641 ctccagcctc tacagtatat gtccgtttct gggaagacgg aaacaataac aacggaacat
    14701 ttggaatctg tgtaattgag cctccctcaa acgatgaacc cactaccgcc acagctttaa
    14761 ccccgggcac cagttgttcc ttttccacct tcacgaattt gaatgcaaca gcaagtacat
    14821 gtggaaccat tccggcaccg ggttgtgcaa cttatgcagg tggtgatgtt tggttcagta
    14881 ttgtagttcc tgccacagga tcactgataa tatcatcgca ggcaggagtt attaccgatg
    14941 gtggtatggc gctttataca ggaacgccat gtggtacatt atcgctcgta agctgcaacg
    15001 ataacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncaacg ataacagcaa
    15121 ccaatttaac tccatcctca acggtatatg ttcgcttttg ggaagacggg aacaataaca
    15181 acggaacatt tggaatttgc gtaattcagc cccccgcaaa tgatgaaccc tcgactgcta
    15241 tttcactaac tcccggggca acttgttcct tttccacatt cactaacctg aacgcaacta
    15301 ccagcacctg cggcaccatt ccagcgccgg gatgtggagg atacagtggt ggagatgttt
    15361 ggttcagttt tgttgttcct ccttcaggat atgttaccat aagcgcacaa gcaggtgtaa
    15421 ttacagatgg tgcaatggcc atatattcag gctctccttg cggtatattg acgttaatta
    15481 gctgtaatga caacggaaca gccatgccga ccattacctc gaatacactt acacccggag
    15541 caaccattta tattcgcttt tgggaagacg gaaataacaa caacggaaca tttggaattt
    15601 gcgttaccga tccgtgttct tcatccagtc cggttaataa cgattgcagc accgcaactc
    15661 ccttaaccgt atatgcatct tcctgtgttc ccttacccgg aacggtacaa tgtgcctctg
    15721 cctctgctga tgcaaacagc tgtggagcaa ctgctgacga tgatgatgtt tggtactcat
    15781 ttgttgccac atcaaccagt gtaaatatta caataaccag tgtagcaggt tcagttaccg
    15841 acatgtatca ttcgctattt accgggacct gtggttcatt gggaaatgcg gtactttgca
    15901 gtgatcccaa ctccagcaca ccaaccggat taaccattgg acaaacctat tatatccgtg
    15961 tttacacggt tagcacagcg cccggacaaa ctactacatt tcaaatttgt gttacccaaa
    16021 tgagtccatg tggaaatccg atgaacaacg atttttgttc cactcctgca actttatcat
    16081 tgagtgcatc aagtactttt tccagcacaa cagcaagcag ctatactgca gataatccgg
    16141 caaacatcaa taccgcattt tcctgcggaa cgattcaaaa taattcctgg tatcagttta
    16201 ctgctacagc aacatcacac actattaaca tcagttcaat tgccggatgt accggaggaa
    16261 tacaagcgca ggtttatgct gttacccgaa atgcaaataa ctgttgtacc aatttcagtt
    16321 ctgtttccaa ttgttatagc cccggcaata caaatccagg cactcttacc gcaaatggac
    16381 ttactattgg aaatacttac gtgctgatga tagatggttg gtcaggtgca aactgcaatt
    16441 atacaatagc aggatggtct gcatctggag ttcttcctat tgaacttatt tcatttgatg
    16501 gaatgaatat tggtaacgga aatgaattac gatggaaaac tgcaactgaa agcaataacg
    16561 aacgattcga tattgaaaga tcgtatgatg gcagtaattt catagttatc ggatcagtgg
    16621 ccgggtcagg gaactcaacg gttccgctgt catatcaatt cttcgatggc aataccgtac
    16681 aaggtcatac ttattaccgc ttacgtcaat tcgattttaa cggggagaac agcacatcgc
    16741 aaaccattgt tgtgtttcca aaagaaaaca acgaagcatt tatttatcct aaccctgcaa
    16801 gtgataaact gaacattgat gtttacaacg aattcaatac ccggataact gttcgcataa
    16861 ttgatcttgc aggacatgtt tttgaagaaa catttttcgc agaagaagga gtaaatcact
    16921 ttaacagcag tcatttttct tc
//
LOCUS       DAOMYG010000013        16735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1196_length_16735_cov_10.124940, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000013 DAOMYG010000000
VERSION     DAOMYG010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 16735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16735
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1196_length_16735_cov_10.124940"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..108)
                     /locus_tag="PK610_01000"
                     /pseudo
     CDS             complement(1..108)
                     /locus_tag="PK610_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(142..315)
                     /locus_tag="PK610_01005"
                     /pseudo
     CDS             complement(142..315)
                     /locus_tag="PK610_01005"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
     gene            complement(1842..>3681)
                     /locus_tag="PK610_01010"
     CDS             complement(1842..>3681)
                     /locus_tag="PK610_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ34530.1"
                     /translation="QIYIAGWGGTLAGLAPYQALGSSTVGLPITSDAIQSSTDGNDFY
                     LMILAPDAQSLLFGTYFGAPSPSNEHVDGGTSRFDKDGVVYQAVCGGCGGQSNFPTTP
                     GAWSNTNNSPNCNLVVFKIDLKEIVAQAQFNFVGDRCNPPVGIQFDNTSTGAISYEWN
                     FGDGTTSTLENPSHTFAAPGTYQVTLIALDSNTCNGVDTALLNVFIPGPLSVAVSPGD
                     TICNGESTVAQVIGGATYTWTPSTGVSGGNTATPTLSPQVSTTYTVIAIDTNGCADTQ
                     TVHVHVQQFVNAGLNAVFTPCSIPVQVSFINESANAVQYFWDFGNGMTSVQPDAQITY
                     TNPGYYDITIVAVDPSTCNGSDTASMQIYLPPPAEITVTGTDTICSNQEAQLFVSGAE
                     SYTWFPPFDINDPTLPNPWVGPDFTTTYGVIGIDTNGCADTGYVTVNVFPPTSIDAGS
                     NIILDIGDAPVLNPSLPSSGTFFWSPSTGLSCTDCQNPVATPDFNQWYYLTYTDLYGC
                     TYVDSLQVLVTPSVFIPNAFSPNDDAHNNVFYPIVRNLATYEFWIFNRWGEVIFHTTD
                     QNEGWNGRHKNNVMCPNDVYVWKIKYTDYIQPDEERVKIGHVTLVK"
     assembly_gap    3682..3781
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3782..5719)
                     /locus_tag="PK610_01015"
     CDS             complement(<3782..5719)
                     /locus_tag="PK610_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34531.1"
                     /translation="MMKFFRLLVVVMTWCLLLSEARAVHPDIFFSPNRGQWHSNISYQ
                     LKMNPGFMYLERKGITFLFLEAEGHHDGDQQHVHEGPERAHALKIDFPGASDEVRFAE
                     GKHVSFYENYFLGNDPAKWQSQLYPAMEVEQKNVYPGIELRYYGTEQGELKYDYVLAP
                     QADYTLIRRVYSGADQLFLKQGELHIVTSIGTVIEKRPKAFQLINGKQKEIPCEFALR
                     GTEVTFVFPKGYDRSKELIIDPVLVFSTFSGSTADNFGFTATYDNQKNSFGGGIIFPT
                     GQYPVTAGAFQSGFSGTPSIGNTTRDIGITKFNVNGNNLIYSTYIGGTGTEAPHSIVC
                     NNAGELYVMGTTSSSNFPMAGTPYDNSFAGGGAATPPSSGMDYAAGSDIFVLRLNPAG
                     TALLASTYVGGTGNDGLNLSTALAYSYGDVFRGEIIVESTGNVVIASVTGSANFPVTG
                     NAPEPLFGGGNYDAVVFRLSANLSALMWSTYLGGSNADAAYGVQEDDNGDIYVSGGTQ
                     SFDLIVTPGVINPGFIAGVDGFVCRYSANGNSILAATYLGTLNYDQAYFVQIDSDGDV
                     YVLGQTTGTYPISPGVYNNNNSGQFLHKMNNTFTTTLWSTRIGRNTGNVDFSPSAFLV
                     NNCKQIYIAGWGGTLAGLAPYQ"
     gene            6097..7998
                     /locus_tag="PK610_01020"
     CDS             6097..7998
                     /locus_tag="PK610_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRJ34532.1"
                     /translation="MSDNIKHECGIAVLRLLQPLEYYVDKYGTAFYGLNKMYLLMEKQ
                     HNRGQDGAGLANIKFDMQPGQRYISRKRSINTNAIQEIFSDVYGKINNGTAKDPSLLK
                     DIPYLKENFPFMGELFLGHLRYGTYGKNSIESCHPFLRQNNWTTRNLVVAGNFNLTNV
                     DELFDHLTELGQHPKEMSDTVTVMEKIGHFLDVENERLFKIYKALGYNNRQISGMIAA
                     NMDIQSILAQSAEKWDGGYAMAGLIGHGDAFVLRDPNGIRPAFYYHDNEICVVASERP
                     VIQTAFNLPVDAIQEITPGHALVIRKNGQIGEFKITEPGEKRSCSFERIYFSRGSDQD
                     IYRERIQLGRNVVPQILETIQYDLDNTVFSYIPNTAETAFYGMIKGVEDYLNGKKKEL
                     ILALGQNPDEKKLEEILAMRPRVEKLAVKDAKLRTFITNDDARDDMVAHVYDITHGTV
                     KKNIDNLVVIDDSIVRGTTLRKSIIKMLDRLSPKRVIIVSSAPQIRFPDCYGIDMAIM
                     GDFIAFQAAVELLRERNQSGLLDEVYLMAKEQLRMPREQARNVVQKVYDAVSMEDISD
                     KVGQLVTPPDCKAEVKVIFQTIDSLHKACPNHSGDWYFSGNYPTPGGRRVANRAFVNF
                     MEGKKVRAY"
     gene            complement(8182..8805)
                     /locus_tag="PK610_01025"
     CDS             complement(8182..8805)
                     /locus_tag="PK610_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HRJ34533.1"
                     /translation="MKNRIAKIAASFLVAVTLFSCGSSRFTRSQYGHRDWSRGGYEAV
                     EQTGMEQSSSSRKNDQKPAEAAAEKKEIPTVENVELIPAAQTEKSVVPATKKGNNNEN
                     YVDAPAAITETTTETVATEAEKAEAEKNNDATNGADTNTILLVILALFIPPLAVFLKD
                     GSVSTMFWITLILCIIGGGFLWGWGGYFGLGYLIAIILAILYVLDMI"
     gene            complement(8835..9527)
                     /gene="purQ"
                     /locus_tag="PK610_01030"
     CDS             complement(8835..9527)
                     /gene="purQ"
                     /locus_tag="PK610_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRJ34534.1"
                     /translation="MKFGVVVFPGSNCDEDMVYVLEKQMGQQVVRLWHKDTDLKGCDF
                     IVLPGGFSYGDYLRSGAIARFSPIMDKVIAHAEKGGYLMGVCNGFQILTEAQLVPGAL
                     LHNTERKFICKNVYIKPESSSALITASLDKNKAYKIPIAHGEGNYFCDADTLVAMKKN
                     DQILFRYCDAEGKITPEANPNGALENIAGVCNAKKNVFGMMPHPERAADEVLGNVDGK
                     AIFESILSMVLK"
     gene            complement(9582..10562)
                     /locus_tag="PK610_01035"
     CDS             complement(9582..10562)
                     /locus_tag="PK610_01035"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HRJ34535.1"
                     /translation="MILRFYRSNQTVVQLALPLIVSVLWSLRIAFPFPVEALPDHSVF
                     SWVPDLPPYKVRIIEALVVLLEALLFNKLINDLELFERVSFVPGLVYVFCAFMLIPDA
                     DFRWVTFSNLFLLPFLRLILVVYRQPDVREESFWAGLSLGVAFCFSWTLAPLLLCGWI
                     MMMVLKTVNWREWMMMIFGMLFPMALVQVIHYGMYGNWSALLSEQHHLQIRFFLFGDD
                     RAAMICWLLLLLMTIAGFFSMLSRQSRSVIKIRRQRQVIMFLVVFLIGIALFSNGLFG
                     GHPGVLLVIPVSFLVSYLVVHSNVTWISDFMLYGTLLFFLLSRVFPTASF"
     gene            10632..10814
                     /locus_tag="PK610_01040"
     CDS             10632..10814
                     /locus_tag="PK610_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34536.1"
                     /translation="MDWLVYPLGDLFVWTFGILEALGNGPNDLFLLIGFAGLFYWLWL
                     QGKFNKEAAANPNQLK"
     gene            complement(10847..12139)
                     /gene="purD"
                     /locus_tag="PK610_01045"
     CDS             complement(10847..12139)
                     /gene="purD"
                     /locus_tag="PK610_01045"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRJ34537.1"
                     /translation="MNVLLLGSGGREHALAWKIAQSSQLEQLFVAPGNAGTSDVATNV
                     NISPTDFKAIRKFVLENKITMVVVGPEDPLVKGIHDFFLADEELKKIPVIGPQKIAAQ
                     LEGSKDFAKAFMQRHGIPTARYQTFSKDTVAEAEAFLDTLKPPYVLKADGLAAGKGVL
                     ILKDLKEAKKEIRSMLTDAKFGEASSKVVIEEFLNGIEVSVFVLTDGKSYKILPEAKD
                     YKRIGEGDTGLNTGGMGAVSPVPFADRDFMDKVENRVIIPTVKGLQQESIPYKGFIFF
                     GLISVKGEPYVIEYNCRLGDPETEVVIPRIKSDILDLFEGVASETLSERDVQFDEKTA
                     VTIMLVSGGYPGDYDKGKTIRGTEGITDSLVFHAGTSEKGGKVVTNGGRVIAVTSYGK
                     DIESAVERSLKNADVIDYDKKYFRRDIGKDLMKMPQQV"
     gene            complement(12442..13098)
                     /locus_tag="PK610_01050"
     CDS             complement(12442..13098)
                     /locus_tag="PK610_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ34538.1"
                     /translation="MHRILLVDDHQLILDGLRSIVSSEKDFEIVAEANDGETAMKQCL
                     ALNPDVVLMDIDMPVMNGLVATTEILKQKPECRIIILTMHDEPALIRKIKEIGASGYL
                     IKNTDQTELITAIRKVLDGEKYFSEAVLAALENGNGQSDEFTLRPTDAVLMSQLTERE
                     IEILRLISDGMSNKEIGDKLFISHRTVDTHRTNLMKKLDVHNIAGLIRFSIRNGLIRS
                     "
     gene            complement(13185..15146)
                     /locus_tag="PK610_01055"
     CDS             complement(13185..15146)
                     /locus_tag="PK610_01055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRJ34539.1"
                     /translation="MLRCLFFVLLAFFVLPVSSDASQLDSLVQVANTMKEDTNKVKLL
                     SDLCFYHRSVHSDTAMNFGLKALRLAEKLKWKKGVAQALNDIAIVHMDKNEYPAALQK
                     LESCLSIRKELKDDLGVAAVYNKMGIIYQEQFKLNDALGVNYNALEIYERLGHKFYQS
                     YIVNNLAIIHFNLRDYEQSLVMHNRALALRVEMGDEYGIAATHGGMANVYYETKDTAR
                     AIENWELAIAGFRKMNKPMELAVQLNNYGGLLVRRKEYKKALPMLEECLAIRNRLNDR
                     KDIASVLINLGEAQMQTGKYRDALGTFHQSLNYSRTIQARHEQLFAYMKLSRVHGFLN
                     RPDSAFFYLEQYSLLKDSVFNEDLGEQVAEMRTRYETEKKEKELLEEKARTEQLAREK
                     AESELIASNRMKWLWAIGGGAIALVFLILFLAQRRQRHLQAEKDAAIISEREKGIEAV
                     ITATEDERKRIARELHDGIGQQLGGLKLAFQQLSKKVEQSLPSEQKLVADLTKILDDS
                     ATEVRAISHQMMPKVLQEVGLVPAIDDMLRKSLGHSNVKYELESFGIEGRFAEKVEIS
                     LYRICQELINNIIKHSGASHVIVQVFRNKGYLIMIVEDNGRGFDVKASRDGIGMMNIS
                     SRINTINGDVNFEPSPGSGTVATVRVPVG"
     gene            complement(15150..16694)
                     /locus_tag="PK610_01060"
     CDS             complement(15150..16694)
                     /locus_tag="PK610_01060"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007651530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRJ34540.1"
                     /translation="MQNKLDLLASKLDEAQLGGGQARIDAQHKKGKLTARERVHFLLD
                     ENSFEEIGALVVHRSSDFGLDKEKFLGDGVVTGYGTIDGRQVYVFAQDFTVFGGSLSE
                     THAEKICKIMDLAMQNGCPVIGLNDSGGARIQEGVVSLAGYADIFYRNTLASGVIPQL
                     SAIMGPCAGGAVYSPALTDFILMVEQQSYMFVTGPNVVKTVTHEEVSSEELGGASTHA
                     SKSGVTHFTAANEIECIQQIKKILSYIPLNCEEQPPFLAYENSGSETRAALNSIVPEN
                     PNQPYDMREVISNVVDNDSFFEVHKDFAENIVVGFARIAGRSIGVVANQPAFLAGVLD
                     IKSSQKGARFVRFCDCFNIPLLVFEDVPGFLPGTDQEWNAIITNGAKLLYAFSEATVP
                     RVTVITRKAYGGAYDVMNSKHIGADMNYAWPTAEIAVMGAKGAAEIIFRKEISEAKDS
                     AAKLKEKELEYNELFANPYKAAERGFIDEVIRPEQTREKLIRAFRMLENKVVNMPRKK
                     HGNIPL"
BASE COUNT         4703 a         3967 c         3445 g         4520 t
ORIGIN      
        1 gaaggcggcg tgggggccgc cgtagcccat gggcacgccg aagcgctggc tgctgccgat
       61 gacgatgtcg gcgcccatct ggccggggga gcgggtcagg gccactaaaa cacacatcga
      121 aacatccatt ccgtcggcca cttcattttc gctattggaa aaggtttctt ccaccaaatc
      181 attcaccttg ttcaggattt cattcggcat ttgcaatcca aattcgcgca ctgctctatt
      241 gagggcattg tgtcctaaca cactcaccat cgcgccgggt acgccatgac cggtgcaatc
      301 gcagcaagcg aacatgattt tacgaccacc ttttattttc tgaaccgatt cgagccagta
      361 aaaatcaccc gcaacgatgt cctttggttt ataaagaatg aatgaatcat ccagaaattc
      421 cttcaccagt ttattcgggg gaagaatagc atcctgaatg cgtttagcgt aacggatact
      481 gtcggttata ttgcggtagg cttcgcttac ttcttcgtgt ttgagttgca attctttaga
      541 ttgtagaagg attttattct tcagcgagcg cgtcttgaaa aacatccaca aactcaatcc
      601 tgcaatgact aaaataaaaa gcaccgaaaa ccacaacaca ccaatttgca tacgcgcatc
      661 ggcaccgcgt agtttttctt cggtcagctc cacatttgtt tcggccagct gggtttcttt
      721 tttcgtaagc aacacttttt tctccagttc attgatgcgg ttcacccgtt caagattgta
      781 caaggtgtcg cgtaaggaat tcaactccga ctgaaacctg aatgcgctgc ggaaatcatt
      841 attctcactg taaaaaccaa ccaattcttc gagggcttca aagcgttcga aatcaaggtg
      901 atgttttctg ctgaatgata aactctccag gaataatttt tctgccttct ttttgcgcat
      961 agcttttgcg taagctttac ccattaacat ggttatggtg gcataactat cgttcatctt
     1021 ttcatccact gccacatcac gcgattgttc gtaccaggaa atagctccgt cgaaatctcc
     1081 ttttagtaat gcaaacgttc cgttgttctg gtatagaaag taatgatcga caggacgcat
     1141 ccagctttcc agattattca attcctccaa agcccaaagc gcgttttttt catccttgtt
     1201 gcgcatggca tcgtttgaat aattgagcaa tgcattgatc agtccgggat gatcattagc
     1261 ctcttcgtac aagcgaatgg attcttttaa tgcacgtgtt tccgcttccg cattgtttaa
     1321 tcttccgtaa agaattgccc gcaacttata aactttcgcc tgaccactca ggttattgct
     1381 gcgttcataa tattctttgg ctttatccag ttcgctgttg gcttcttcgt aacgcgtttt
     1441 gagcaaaaaa tacctcgcct tgttcaattc agcatgcgca atcatttctt tatcctgcat
     1501 caataatgcg agtttttccg ccttcacact taaagaaaat gaggaatcag gatcagaata
     1561 ataaatggaa tcagcccgct ccaacaaatc acgttgtgct tgcccggaaa aaataagcaa
     1621 caataaaaaa accggaatca tcatcgtccg gacccatctc acacacgctg ccttacttat
     1681 catttccatc ttcaaaatta aattcattac atccctccca aagccttatc gatcctgaag
     1741 cgaaatcgac cgtaaggaga tttctgcttc aggactgttc accggcctgt cccgaccgta
     1801 ccgaagggga gcgtcgggat cactgatcac accattcacc cctacttcac caaagtcacg
     1861 tgcccgatct tcacacgttc ttcatccggt tgaatgtaat cggtgtattt gatcttccaa
     1921 acatacacat cgttcggaca catcacattg tttttgtgtc ttccgttcca gccttcgttt
     1981 tgatcggtgg tatgaaaaat aacttctccc cagcgattga agatccagaa ctcgtaagtg
     2041 gcaagattgc gtacgatcgg ataaaacaca ttgttatgcg catcgtcgtt gggagaaaat
     2101 gcattgggaa taaaaacaga aggcgtaacc agcacctgca gtgaatcgac gtaggtacaa
     2161 ccgtacaaat cggtatacgt taaatagtac cactgattaa aatcgggtgt agcaactgga
     2221 ttctgacaat cggtacaact taatccggta gaaggcgacc aaaaaaatgt tccactcgac
     2281 ggaagagaag gattcaatac cggtgcatca ccaatatcga gaatgatgtt tgatccggca
     2341 tcgatagaag tgggtggaaa aacatttacc gtaacatagc ctgtgtctgc gcaaccattg
     2401 gtatcaattc cgattacgcc gtatgttgtg gtaaaatcag ggccaaccca gggattaggc
     2461 aatgtaggat cattgatatc gaatggcgga aaccaggtgt acgattctgc accgctaaca
     2521 aacaactgcg cttcctgatt ggaacaaata gtatcggtcc cggtaacggt tatttcagca
     2581 ggaggcggga gataaatctg catgcttgca gtatctgatc cgttacaggt agaaggatcg
     2641 acagctacaa tggtgatatc gtaatatccg ggattggtat aggtgatctg cgcatccggc
     2701 tgaacagagg tcatgccatt tccaaaatcc cagaaatact gaacggcgtt tgcactttca
     2761 ttgataaaac tcacctgaac aggaattgag caaggagtga acacggcatt cagtcctgca
     2821 tttacaaatt gctgcacatg tacatgaaca gtttgtgtgt cggcacaacc attggtatct
     2881 atagcaatta ctgtatacgt tgtagacacc tgcggagaga gcgtgggtgt tgctgtattt
     2941 ccaccactta ctcctgttga aggcgtccag gtataagtgg caccgcctat tacctgagca
     3001 acagtagatt caccattgca aatggtatca ccaggactca cggcaacgga aagtggtccg
     3061 ggaataaata cattgagtaa agctgtatca accccattac aggtatttga atcgagcgca
     3121 ataagtgtta cctgatacgt tccgggagcg gcgaaggtgt gcgagggatt ttcaagcgta
     3181 gacgttgttc cgtctccgaa attccattca taggaaattg ctcctgtgct ggtattatca
     3241 aactgaatcc caaccggggg attgcatcga tcgccaacaa aattaaattg tgcctgcgca
     3301 acaatttctt tcagatcgat tttaaaaaca accagattgc aattgggact attattcgtg
     3361 ttcgaccatg cacctggggt tgtaggaaag ttagattgtc cgccgcaacc accacacact
     3421 gcctgataca ctactccatc tttatcaaaa cgcgaagtac ctccatccac atgttcgttg
     3481 gaaggagaag gcgcaccgaa atacgtccca aacaataaac tctgcgcatc gggtgcaaga
     3541 atcatcaggt aaaaatcatt tccatccgta ctcgactgta tggcatctga agtgatcggt
     3601 aaaccaactg ttgaactccc caaggcctgg tagggagcaa ggcccgccag tgtaccgccc
     3661 catcctgcaa tataaatctg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nctggtaggg agcaaggccc gccagtgtac cgccccatcc tgcaatataa atctgtttac
     3841 agttgttcac cagaaatgca gaaggagaaa aatcgacatt cccggtgttg cggccaatac
     3901 gagtcgacca cagtgttgtg gtaaacgtat tgttcatttt gtgcaggaat tgtccgctgt
     3961 tgttgttgtt gtacactccg ggagaaattg gatacgttcc tgttgtttgt ccgagcacat
     4021 acacatctcc atcgctatca atctgcacga aatacgcctg atcgtaattg agtgtaccga
     4081 ggtaagtagc agcaagaatg gaattgccat tggcactgta tctgcacaca aaaccatcca
     4141 ctccggcaat gaaacccgga ttgataacac ccggcgttac gataagatca aaactttgtg
     4201 ttccgccact cacgtaaata tcgccgttat catcttcctg cacaccgtac gcagcatctg
     4261 cattggaacc accaaggtat gtactccaca tcaaagcaga aagattcgcg cttagacgaa
     4321 aaacaactgc atcgtaattt ccaccaccaa acaagggctc aggtgcattg ccggtaaccg
     4381 gaaaattagc agaacctgtt accgatgcga tcaccacatt acctgtgctt tcaacaatga
     4441 tttcccctct aaaaacatca ccgtaactat aggcaagagc agtggagaga tttaatccgt
     4501 cgttgcctgt tcctcctacg taagtagaag caagcaatgc tgttccggca ggattgagtc
     4561 gcaacacaaa aatatctgac cctgctgcgt aatccattcc cgaactaggc ggtgtagcgg
     4621 ctcctcctcc tgcaaatgaa ttatcgtagg gtgttcctgc cattggaaaa ttggatgatg
     4681 aagtggttcc cattacatac aactctcctg cattattgca aacaatagaa tgcggagcct
     4741 ctgttcctgt tcctccgata taggttgaat agatcagatt gtttccgttt acattgaatt
     4801 tggtgatgcc aatatcgcgg gtggtatttc caatggaggg tgttccgcta aacccactct
     4861 gaaaagcacc tgctgttacc gggtattgtc cggttggaaa aataattcct ccgccaaatg
     4921 aatttttctg attatcataa gttgcggtga atccgaaatt atcggcagta gatcccgaaa
     4981 aagttgaaaa caccaataca ggatcaatga tcagctcttt actgcgatcg tatcccttag
     5041 ggaaaacaaa tgtcacctcg gtaccacgca atgcaaattc gcagggaatt tctttttgtt
     5101 ttccgttaat caactgaaat gctttcggac gtttttcaat aacggttccg attgaagtga
     5161 cgatgtgtaa ttctccttgt ttgagaaaaa gctgatctgc tcccgaatat acacggcgaa
     5221 tcaaggtgta atcggcctga ggagccaaaa cataatcgta tttcaattcg ccctgttcgg
     5281 ttccgtaata gcgcaattca atgccgggat acacattttt ctgctccact tccatagcag
     5341 gatacaattg cgattgccat ttggcaggat cgttacccag aaaataattt tcgtaaaacg
     5401 aaacatgttt accctcggca aaacgcacct catcgcttgc tcccggaaaa tcgatcttca
     5461 gcgcatgtgc ccgctccgga ccttcatgca cgtgttgctg atcaccatcg tggtgacctt
     5521 cggcttcaag aaacaaaaaa gtaataccct tgcgctcgag gtacatgaat ccgggattca
     5581 tcttgagctg gtaagaaata ttcgaatgcc actgtccgcg attcggagaa aagaaaatat
     5641 ccggatgcac agcacgtgcc tcagaaagaa gcaagcacca ggtcattacg accactaaca
     5701 aacggaagaa cttcatcatt atgaaacagc aagttacgaa ccattccaca gacgcacaaa
     5761 acaactatat gttgccccta agaatgccaa taataaagca aaggcaggaa taccttcgtc
     5821 aacggatgaa gattgtcatt caacacattg tttttcaatt acttaataaa actccaattc
     5881 cctttgacca attcccttac actgtccttc gtatgccgta acgcattgaa aggagggtcc
     5941 tcttgcactc ttccgggttt tagatcccat ttccgattat taccatctca tagccttgac
     6001 ggataagaat tcgcacattg attttgaaaa ttgtcaaaat caatcctgac cgaagcgtca
     6061 ggattctcct tacctttgca ctccagttct tcccctatga gcgacaacat caaacacgaa
     6121 tgcggtattg ccgtactgcg cctacttcag cctctggaat attatgtcga caaatacggc
     6181 acagcttttt acgggctgaa caagatgtac ctgctgatgg aaaaacaaca caaccgcgga
     6241 caggacggtg caggactggc gaacatcaag ttcgatatgc aaccgggtca gcgttacatc
     6301 tcacgcaaac gctctatcaa caccaatgca atacaggaaa ttttttcgga tgtatacgga
     6361 aaaataaata atggtactgc aaaagatcct tcactgctca aagacattcc ctacctgaaa
     6421 gaaaatttcc cgttcatggg tgaattgttt cttggtcact tgcgctatgg tacctacgga
     6481 aaaaacagca ttgagagttg tcaccctttt cttcggcaga acaactggac aacacgaaat
     6541 cttgttgtag caggaaattt caatctcacc aatgtcgacg aacttttcga tcatctcacc
     6601 gaattgggtc agcaccctaa ggagatgtcg gatactgtta ctgtaatgga aaagatcggt
     6661 catttccttg atgtggaaaa cgaacgtctc tttaaaattt acaaagcact tggatacaac
     6721 aaccgtcaga tctcaggcat gattgctgcg aatatggata ttcaaagtat cctggcgcaa
     6781 tcggcagaga aatgggatgg tggttatgcc atggcaggat tgattggtca cggcgatgca
     6841 tttgtgcttc gcgatcccaa tggaattcgt cctgcgtttt attaccacga caacgaaatc
     6901 tgcgtagtgg catccgagcg tcctgtgatt cagacagcct tcaacttacc ggtagatgcc
     6961 attcaggaga tcacacccgg acatgcgctg gtgattcgta aaaacggaca gatcggtgaa
     7021 ttcaaaataa cagaacccgg agagaaacgt tcatgttcat ttgagcggat ttatttttcc
     7081 cgcggtagtg atcaggacat ttaccgtgag cgcatacagc tcggaagaaa tgtagttccg
     7141 caaattcttg aaacgatcca atacgatctt gataatacgg tattctctta cattcccaat
     7201 actgcagaaa ctgcatttta cggaatgata aaaggtgtgg aagattacct taacgggaaa
     7261 aagaaagaac tcattcttgc gctcggacaa aatcccgacg agaaaaaact ggaagagata
     7321 ttggccatgc gtccacgtgt tgaaaaactc gcggtaaaag atgctaagct acgcaccttc
     7381 atcaccaacg acgatgcacg cgacgacatg gttgcccacg tgtacgacat tacccatgga
     7441 accgtaaaaa agaacatcga caatttggtt gttatagacg actccattgt tcgcggaaca
     7501 acactgcgaa aaagcattat taaaatgctg gatcgtttat caccaaaacg cgtcatcatc
     7561 gtttcttccg ctcctcaaat tcgttttccc gactgttacg gaatcgatat ggccattatg
     7621 ggagatttca ttgcatttca ggcagctgtt gaattgttac gcgagcgcaa ccaatcagga
     7681 ttactcgacg aagtgtatct gatggccaag gaacaattgc gtatgccccg cgagcaggca
     7741 cgcaacgtgg tacaaaaagt gtacgatgcc gtttcaatgg aagacatctc ggataaagtt
     7801 ggtcagcttg taacaccacc cgattgtaaa gcagaagtaa aagtgatttt tcaaacgata
     7861 gacagtctgc acaaagcatg tcccaaccac agcggcgact ggtatttctc cggaaactac
     7921 cccacccctg gcggacgccg tgttgccaat cgtgccttcg taaacttcat ggaaggaaag
     7981 aaggttagag cttattgagg tgaacagtcc tgaagcgaaa tcaactgtaa ggagatttct
     8041 gcttcaggat agatttcgct tcaggataga ttaggctttg gaaggaggtt ttgggtcaag
     8101 gtgaattacc gataatagaa aactacaaaa caaaaaatcc cgatcatttc tgaccgggat
     8161 ttttttattt gcgttatgac attatatcat atcgagcaca taaagaatag cgaggataat
     8221 tgcaataagg taacctaatc cgaaataacc gccccagccc cataaaaatc ctcctccaat
     8281 gatgcatagg ataagggtaa tccagaacat ggttgaaaca gatccgtctt taaggaatac
     8341 agccagtggc gggataaata atgcaagaat caccaataag atcgtatttg tatctgcacc
     8401 gttggttgcg tcgttgtttt tttctgcttc tgctttctct gcttcagtgg caacagtttc
     8461 agtggtagtt tctgtaattg cagcaggggc gtcaacataa ttttcattgt tgttaccctt
     8521 tttagttgca ggaacaactg atttttctgt ttgcgcagca ggaataagct ctacgttttc
     8581 tacagtagga atttcttttt tctctgctgc agcttcggca ggtttctggt cgtttttacg
     8641 cgatgaagag gattgttcca tccctgtttg ctcaacagct tcgtaaccgc cacgtgacca
     8701 atcacgatgt ccgtactgcg aacgcgtaaa acgcgatgat ccgcaagaga agagtgttac
     8761 tgctaccaaa aatgaggcag caatcttggc aattcgattt ttcatggttg tttggtttat
     8821 gaggtttccg attattattt caataccata gaaagaatgg attcaaaaat tgcttttcca
     8881 tccacatttc ccagcacttc atctgctgcc cgctctgggt gtggcatcat accgaataca
     8941 tttttctttg cattacaaac tcctgcaatg ttctccaatg caccattggg gtttgcttcg
     9001 ggggtaattt taccctctgc atcacaataa cggaagagga tctgatcgtt cttcttcatt
     9061 gctacgagcg tgtctgcatc gcaaaaataa tttccttctc cgtgtgcaat cggaattttg
     9121 taggctttgt ttttatccag cgaagctgtg atcagcgcag aagaagactc gggtttgata
     9181 tatacatttt tgcagatgaa tttcctttcg gtattgtgca aaagcgctcc gggcaccaat
     9241 tgtgcctcag taagaatctg gaatccgtta caaacaccca tcaggtatcc tcccttttca
     9301 gcgtgagcaa tcactttatc catgatcgga gaaaaacgtg caatcgctcc tgaacgcaaa
     9361 taatcgccat aggaaaatcc gcctggcaga acaataaaat cgcagccctt cagatcggta
     9421 tccttgtgcc atagccgaac gacttgctga cccatttgct tttccaggac atacaccatg
     9481 tcctcatcac aattcgatcc cgggaaaact actactccga atttcatatt gcatttagtt
     9541 ttgatcaaaa ttacacaaat actccctaag ggaaagcact gttaaaaact tgcagtggga
     9601 aaaacccttg aaagcaggaa aaacaagagc gtaccgtaaa gcataaaatc acttatccac
     9661 gttacattcg aatgcacaac cagatacgac accagaaaag ataccggaat aaccaataac
     9721 actccgggat gtccgccaaa gagtccgttg gaaaatagtg caattccaat cagaaaaaca
     9781 accaaaaaca tgatgacctg ccgctgacgg cgtattttga tcaccgatct actttgccgg
     9841 gagagcatgg agaaaaatcc agcgatagtc atcagcaaca gcagcaacca gcaaatcatt
     9901 gcagcacgat cgtcgccaaa gagaaaaaag cgaatttgca aatgatgttg ttcagaaagc
     9961 aaagcactcc agttgccata cattccgtaa tgaattacct gcaccaatgc cattgggaaa
    10021 agcattccga aaatcatcat catccattcc ctccagttaa ccgttttcag taccatcatc
    10081 atgatccatc cgcaaagcaa gaggggcgca agtgtccagg aaaaacaaaa agccacacca
    10141 agcgacaatc cggcccagaa ggattcctct ctcacatccg gttgtcggta aacaacaagg
    10201 ataagacgca aaaaaggcaa cagaaaaaga ttggaaaaag taacccatct gaaatcagca
    10261 tccggaatca gcatgaacgc acaaaaaaca tacaccagcc ccggtacaaa tgaaacacgc
    10321 tcaaataact caagatcatt gatcagttta ttgaacaaca gtgcctccag taataccaca
    10381 agggcctcga taatgcgcac cttgtaggga ggcaggtcgg gaacccatga aaagacagag
    10441 tgatcaggta aggcctccac cggaaaaggg aaggcgatgc gtaatgacca caaaacactt
    10501 acaatcagag gcaaagccag ctggacaaca gtctgattgg agcgatagaa tcgtagaatc
    10561 acgaggataa agttaatttt tcttttttca gcgaaactgc tacatttgac ccaacatcaa
    10621 aactcttcat tatggattgg ctggtatatc ccctcggtga tcttttcgtt tggacttttg
    10681 gcattcttga agccttaggt aatgggccta acgatctgtt ccttttaatt ggctttgcag
    10741 gcttgtttta ctggctttgg ctacaaggca agttcaacaa agaagctgct gcaaatccca
    10801 atcagctgaa gtaaattttc tctgtattta tcaagtgccc cacaggttaa acctgctggg
    10861 gcattttcat taggtcttta ccgatgtcgc ggcggaagta ttttttatcg taatcaatca
    10921 catccgcatt tttaagtgaa cgctcaactg ccgattcaat atctttccca tatgaagtaa
    10981 ccgcgattac gcgtccgcca ttggtcacca cttttcctcc cttttcgctt gttcccgcat
    11041 gaaataccaa tgagtcagta attccttccg ttccgcgaat ggtttttcct ttatcgtaat
    11101 caccagggta accacctgaa acgagcataa tggtaacagc agttttttca tcgaactgca
    11161 catcgcgttc agagagggtt tcagaggcaa cgccttcaaa caaatccaaa atatcgcttt
    11221 tgatgcgtgg aataaccact tcggtttccg gatcgcccag tcggcaattg tattcgatca
    11281 cataaggctc gcccttcaca ctgattaatc cgaaaaaaat aaatccctta taaggaatac
    11341 tctcctgctg caaacctttt acggttggaa taatgacacg gttttccacc ttatccataa
    11401 aatcacgatc ggcaaacggc accggggaaa cagctcccat gccgccggta ttcaatccgg
    11461 tatcgccttc gccaatgcgt ttgtaatctt ttgcttcggg taagatcttg tatgattttc
    11521 catctgtcag gacaaaaacc gacacttcta ttccattgag aaattcctcg atcaccactt
    11581 tgcttgatgc ttctccgaat tttgcgtcgg ttagcatcga acgtatttcc tttttcgctt
    11641 ctttcagatc tttcagaatc aacactccct tacccgcagc caatccgtcg gccttgagca
    11701 cataaggtgg ttttagagta tcaagaaatg cttcagcctc tgcaacagtg tccttgctga
    11761 atgtttggta acgcgcagtt ggaattccgt gacgctgcat aaaggccttg gcgaaatcct
    11821 tgcttccttc gagctgtgca gcgatctttt gcggaccaat aaccggaatc tttttcagtt
    11881 cttcgtcggc aagaaaaaaa tcgtgaatgc ctttcaccaa aggatcttca ggtccaacta
    11941 ccaccatagt gatcttgttt tcgagaacga actttcgaat ggctttgaaa tcggtcggac
    12001 tgatgtttac gttggtggca acatcagaag taccggcatt ccctggcgct acaaagagtt
    12061 gttcgagctg tgagctttga gcgatcttcc aggcgagcgc atgctcacgt ccgccggaac
    12121 cgagaagaag tacattcatg agaattccat tttttcactt acaaagaaag agtaaagtaa
    12181 gcgctcatgg aaatctgttt atcaacacgc tgtgtacatc taaaaaacct gcaccctcct
    12241 ccttgaaaaa cggaacctca aacagaggaa gatgcagggc ttgtttagct ttttcgatgt
    12301 atcgattttc tgtttacgga actcttttta aaacatgaaa agcatcccat agaaaaaatc
    12361 tcattggaag tttcgggttt ctcggttgta caaagaaccc tgtttatcaa aaaaagagaa
    12421 atacggtaaa tcccgtaata ttcaggagcg gatcaatccg ttgcggatgg aaaagcggat
    12481 tagtcctgca atgttgtgca catcaagctt cttcatgaga ttggtgcggt gcgtatcaac
    12541 tgtgcgatgc gaaataaaca gtttgtcgcc gatttcctta ttcgacattc catcggaaat
    12601 gagacgtaaa atttcaatct cacgttcagt aagctgactc atcaacacgg catcggttgg
    12661 acgaagggta aactcatcgg attgcccgtt gccattttcc aatgcggcaa ggacggcttc
    12721 tgaaaaatat ttttctccgt cgagtacttt ccggatggcg gtgatcaatt ctgtttggtc
    12781 ggtattcttg atcaaatatc cactggcgcc gatctctttg attttacgaa tcaacgccgg
    12841 ttcgtcgtgc atggttagaa tgatgatcct gcattcgggt ttttgtttga ggatttctgt
    12901 tgttgcaacg agtccgttca ttaccggcat atcgatatcc atcagcacca catcaggatt
    12961 caaggcaaga cattgcttca ttgcagtttc accgtcattt gcttctgcaa caatttcaaa
    13021 gtccttttca gagctaacga tcgaacgtaa tccatccagg atcaattggt ggtcgtcgac
    13081 cagtaaaatt cggtgcatgc gcaaacaagt tgaggttccg gcattaaatg taacccgaat
    13141 ttatgactgc atgaaaggat gcgaaaggga aattcttaac aatttcagcc aacaggaacc
    13201 cgcactgtag caaccgtacc ggagccagga ctgggttcaa aattcacatc gccgttgatg
    13261 gtgttgattc ggcttgaaat attcatcatc ccgatcccat cacgactggc tttcacatcg
    13321 aatccacgac cgttgtcttc aacaatcatg atgaggtaac ccttatttcg gaatacctga
    13381 acaatgacat ggctggcacc cgaatgtttg atgatgttgt tgatgagttc ctgacaaatt
    13441 ctatacaggc tgatctccac tttttcggca aagcgacctt caatgccaaa cgattccagt
    13501 tcgtatttca cattggagtg accaagactt ttcctgagca tgtcatcaat tgcaggtaca
    13561 agtccaacct cctgcaacac ttttggcatc atctggtgcg aaatggctct tacctctgtt
    13621 gcagaatcgt cgagtatttt ggtaagatca gccaccagtt tttgttccga aggaagtgat
    13681 tgttctactt ttttactcaa ttgctgaaat gcaagtttca atccacccag ttgctgacca
    13741 ataccatcgt gcaattcacg tgcaattcgt ttacgttcat cttcagtagc tgtgataacg
    13801 gcttcaatac ctttttcccg ctcgctgatg atggctgcat ctttttcagc ctgtaagtgg
    13861 cgttgacgcc gttgagcgag gaacaggatc aaaaacacga gagcaattgc accaccacca
    13921 atggcccata accatttcat gcggttggat gcgatcagct ctgattcagc tttttcacgt
    13981 gccagctgtt cggtgcgcgc tttttcttcg agcaattctt tttctttctt ttcggtttcg
    14041 tacctggtac gcatctccgc tacctgttcg ccaagatctt cattgaagac cgaatccttt
    14101 aacagggaat actgttccag gtaaaagaaa gcactgtctg ggcgattgag aaaaccatgc
    14161 acccgcgaaa gtttcatata cgcaaacagc tgttcatgcc tggcttgaat tgtacgtgaa
    14221 taattgagcg attgatgaaa tgtgcctaaa gcatcacggt atttccctgt ttgcatttgt
    14281 gcctcaccca gattaatcag caccgacgca atatccttgc gatcatttaa acggttgcga
    14341 atagcaagac attcctccag catgggaagt gcctttttgt attcctttcg ccgcactagt
    14401 aatccaccgt aattattcaa ctgaactgcc agctccatcg gcttgttcat ttttcgaaat
    14461 cctgcaatag ccaattccca attttcgatt gcgcgggctg tgtctttcgt ttcgtaatac
    14521 acattcgcca taccaccgtg cgtggccgca attccgtatt catcacccat ctccactcgt
    14581 aatgcaagtg cacggttatg catgacaagc gattgctcgt aatcgcgcag gttaaaatga
    14641 atgatggcta gattatttac aatgtacgat tggtaaaatt tatgtccgag ccgctcgtaa
    14701 atttccagtg cattatagtt taccccaagc gcatcattca gcttgaattg ttcctggtaa
    14761 atgattccca ttttattgta cactgcagca acacccagat catcctttaa ctctttacga
    14821 atggaaaggc atgattccag tttttgcagc gctgcaggat attcgttctt atccatgtgc
    14881 acaatggcaa tatcgttcaa tgcctgtgca acaccttttt tccatttcaa tttttcggcc
    14941 agacgcaaag cctttaaacc aaaattcatt gcagtatcgg aatgcaccga acgatgataa
    15001 aaacaaagat cagaaagcaa tttcaccttg ttggtgtctt ctttcattgt gtttgccacc
    15061 tgaaccaggc tatccaactg cgaagcatcg gaagaaaccg gcaacacaaa aaaagcaagc
    15121 agaacaaaaa acaggcagcg caacatagat cagagtggaa tatttccgtg tttctttctt
    15181 ggcatattca ccactttgtt ttcgagcata cgaaaagcac gaatcagttt ttcgcgggtt
    15241 tgttccggac gaataacctc atcgataaat ccacgttcag ctgctttata cggattggcg
    15301 aatagttcat tgtattccag ttccttctct ttcaactttg cagcagaatc ctttgcctcg
    15361 gaaatttctt ttctgaaaat aatctctgct gcacctttag cgcccattac agcgatctct
    15421 gcagttggcc atgcgtaatt catatccgca ccgatatgtt tcgaattcat cacatcgtac
    15481 gcgccgccat aagctttacg tgtaatcact gtaacacgcg gaacggttgc ttcagaaaat
    15541 gcatacaaca atttggcacc gttggtgata atggcattcc attcctgatc cgttccgggt
    15601 aagaatccgg gcacgtcttc aaaaacaaga agcggaatat tgaaacaatc gcagaaacgc
    15661 acaaaccgcg ctcccttttg cgaacttttt atgtccagca ctcccgccag aaaagcaggt
    15721 tgattggcaa caacaccaat acttcttccg gcaatacgtg caaatcccac aacaatgttt
    15781 tcggcaaaat ctttatgcac ttcgaaaaaa gaatcattgt caaccacatt gctgattacc
    15841 tcacgcatat cataaggttg attcggattt tcaggaacaa tactattcag tgctgcccgc
    15901 gtttcactgc ctgaattttc gtaagcaaga aacggtggtt gttcttcgca gttgagcggt
    15961 atataggaaa gaattttttt gatctgctga atacattcga tctcgtttgc agccgtaaaa
    16021 tgagttacac cagatttgct ggcatgcgtt gatgcaccac ccagttcttc cgatgaaact
    16081 tcttcgtgcg ttactgtctt taccacgttt gggccggtaa cgaacatgta cgattgttgt
    16141 tccaccatca aaataaaatc ggtaagcgca ggcgaataca ctgcaccacc tgcacaagga
    16201 cccatgatcg ccgacaattg cggaatcact cctgaagcaa gtgtatttct gtaaaaaata
    16261 tcggcataac ccgcaagtga gaccacacct tcctgaatac gtgctccacc cgaatcattg
    16321 agcccgatca ccggacaacc attctgcatg gcgagatcca tgattttgca aatcttttcg
    16381 gcgtgcgttt cggaaagcga tccgccaaat acagtaaaat cctgtgcaaa aacataaacc
    16441 tgtcgtccat caatggtacc gtaaccggta acaacaccat cgccaagaaa tttttcttta
    16501 tccaatccaa aatcggaaga gcgatgcacg accaaagctc cgatttcttc aaacgaattt
    16561 tcatcgagta aaaaatgtac acgctctctt gcggtgagct ttcctttttt gtgttgtgca
    16621 tcgatacggg cctgaccacc gcccaattgt gcttcatcca gtttggaagc cagcaaatcc
    16681 agtttattct gcatgatgtg gaattaggct ttaaaaatag acaggattcc ccaac
//
LOCUS       DAOMYG010000014        16441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1221_length_16441_cov_10.523496, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000014 DAOMYG010000000
VERSION     DAOMYG010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 16441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16441
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1221_length_16441_cov_10.523496"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1428
                     /gene="ftsA"
                     /locus_tag="PK610_01065"
     CDS             157..1428
                     /gene="ftsA"
                     /locus_tag="PK610_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRJ34541.1"
                     /translation="MESPEIIVGLDIGTTKIACLVGRKNEHGKIEILGVGKSESVGVT
                     RGVVSNIEKTVASIKAAVEEASRQSGVEINVVNVGIAGQHIKSLQHRGIRTRSTIEEE
                     IAQNDIDALIDDMYKLVMLPGEEIIHVLPQEYIVDNEQGIKDPIGMSGIRLEANFHII
                     TGQIAAAKNIHKCVAKAGLEVDELILEPLASSEAVLSTEEKEAGVVLVDIGGGTTDIA
                     IFQDGIIRHTAVIPFGGNVITEDVKEGCTIIHKQAELLKVKFGSALASEAQANEIVCI
                     PGLRGRDPKEISVRNLASIIQARMEEIVEHVYYEIKNSGYEKKLIGGIVVTGGGSQLK
                     HITQLFEYITGMDARIGYPTEHLGKGAEGVTSPMFSTGVGLVLMGFQKYEKQARSKNA
                     VIKTHSQKTKGSFFEKIFGKVGKSFFEEDID"
     gene            1483..>2899
                     /gene="ftsZ"
                     /locus_tag="PK610_01070"
     CDS             1483..>2899
                     /gene="ftsZ"
                     /locus_tag="PK610_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRJ34542.1"
                     /translation="MFKFELPREQASIIKVIGVGGGGSNAVNYMFRHQIKGVDFIVCN
                     TDSQSLEMSPIPTKIQLGASLTQGLGAGAIPEVGRNAAIENIEDIKAILGQNTRMVFI
                     TAGMGGGTGTGAAPVIAQAAREMGILTVGIVTIPFGFEGKKRKQQAEEGLEFMRQAVD
                     TLLVINNDKLREMYGNLTLANAFSQADEVLNTAARGIAEMISVTGLINVDFNDVNTVM
                     RNSGVAIMGSASAAGENRARIAVEKALSSPLLNENHIYGAQYVLLNITYGTQEVTMDE
                     IAEITDYIQEEAGSTADVIWGHGFDEMLGDSVSVTVIATGFNRTPDTGVAMKMPEKNK
                     VILTEETPTAITAPIQSPTQRQTVSESVPEPYLKGGVEEVHAEISESSVALDEPVLKI
                     MDPVNVTPVAENTVSETPITRHTLDTTSIEEITSSIEENVVFTSEKNADLPVSENPEW
                     EITSSVEEETLQFEVRTEAPKT"
     assembly_gap    2900..2999
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3000..3551
                     /locus_tag="PK610_01075"
     CDS             <3000..3551
                     /locus_tag="PK610_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34543.1"
                     /translation="SVEEETLQFEVRTEAPKTIDVSAETPQENVIRHWLDESEDVNDL
                     KAKGPEMNSQQNTPETTGENKQQDVRSEIPSTKGQSTLFPVENVDPEKEKQLSSEEQQ
                     RRAQERLARIKELSSKLKSPAGLNDLENEPAYIRRKITLDKTQHSSESNISRFSVGGD
                     GKDESGNGGGLRSNNSFLHDNVD"
     gene            3553..3996
                     /locus_tag="PK610_01080"
     CDS             3553..3996
                     /locus_tag="PK610_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRJ34544.1"
                     /translation="MNLSEQINADIKSAMLAKEKEKLEALRAIKAALLLEQTKGTGEV
                     SNDAEVKMLQKMYKQRNEAAEIFVQQGRPELAETEIFQANIIKAYLPEQMSEEQVKAV
                     IQEVIASVGASSAADMGKVMGPAMAKLQGKADGKLISAFVKQLLG"
     gene            4178..5260
                     /locus_tag="PK610_01085"
     CDS             4178..5260
                     /locus_tag="PK610_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34545.1"
                     /translation="MIRTLFFVFALAFIAACRRDVPIPDDIPDEDTGVVFNLDQVPYP
                     KLSDYKFFTGALKNLNPNERVLPYEPITPLFSDYAHKSRFVWMMKDSSAQYVSDHSLF
                     DFPDGTVFIKNFYYDNVVPSGERKILETRLLYKKEGEWKFADYIWNQDQSEAVYETNG
                     SYVPVQWIDDQGDNQSLTFRIPSEVECFTCHKSNGNKIPIAPKPQNLDKDFSYPDGVK
                     NQLVKWQEVGYLTGNVPGTIARVADWTDDTQPLETRVRSYLDINCGFCHSDGGHCNYR
                     PIRLEFFKTLDPAMLGVCEIPQQFITPQLTHVIARSNIARSVMHFRMSSTDPQYMMPL
                     LGRSVVHQEAVDLLGQYILSLTPICN"
     gene            5294..7300
                     /locus_tag="PK610_01090"
     CDS             5294..7300
                     /locus_tag="PK610_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRJ34546.1"
                     /translation="MQKRILLSIMTLFSAMHLYAQQSCATAIPAVLGTTTASTSFGTT
                     PPAPAHCMVLNGSESFLWYTFTPAVDTNVRVSTVTSTNTGLDTRISVFSGTCGALSCI
                     AYGDDESAFLSIATFAATAGTTYYIVFDTYWGNMPIDFTISYVSTGGGGGPIPQLITF
                     TTQGIPNGDNSRCVMDMNGDHIDDIVYTSGNTLRVHYQTAPATFTSTTHTISGTVTNA
                     PSWSIAGGDMDNNGYNDLIYGGGSGASIIMANSTGTAYTHYSGGQYIFSQRTNVVDIN
                     NDGIADAFVCHDIDANVFYISNGSGGFTWNQGGLGANGGNYGSVWIDYDNDCDMDLYI
                     AKCGSDPIDQLHRNNGDGTYTSVGVAAGLNDATENWSSTWADFDNDGDMDVFMGASSN
                     WGGLSHKLYRNNGNGTFTNITAGSGFDLYYSNLGIENYPGDFNNDGWVDVYGLGGLIM
                     FNDGDMTFTASTSPVAGAAVGDLNQDGALDLVAGTTIYKNNLTSNNYIVVSTVGVQSN
                     KNGIGARVTVQTASGSQIRDVRSGQAFSSMQTLNAHFGIGQETAIEKITICWPSGVVD
                     VINNPAINSHLVVVEGSTVSVQEELSQNLQVFPNPATSHLSISGLPANRVFALEIFDV
                     TGKLISTQQVSENVTVDVQSLVPGIYVARISGEASQLNVKFIKE"
     gene            7355..8149
                     /locus_tag="PK610_01095"
     CDS             7355..8149
                     /locus_tag="PK610_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ34547.1"
                     /translation="MTNFQNKVVWITGASSGIGEELAYAFSGNGARLVLSARRSSELE
                     RVRNACANPENVFILPLDLEKQEEFPVLVNKVVLQFGRIDFLVNNGGISQRANASETP
                     MEVDRKIMEVNYFGSIALTKAVLPQMKQQGSGTIIVMSSIAGKFGFYLRSAYSAAKHA
                     LHGFYESLRLEEEKNGIRITIVCPGKINTNISLNALSKDGNAHGVMDHNQETGMPADE
                     CAKVILRGVSAGKEELFVGGKELKAVTLKRFFPRLFGKIIRKQKAT"
     gene            complement(8164..9054)
                     /locus_tag="PK610_01100"
     CDS             complement(8164..9054)
                     /locus_tag="PK610_01100"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HRJ34548.1"
                     /translation="MKLFCLSFIALHLICIPELNAQDLLNEIEGDDELQHVRASFKNP
                     KVINAQSLETTHAGVLDFRISHRFGRLNGGLYELFGLDQASLRLSFDYGITDRFQIGV
                     GRSTYEKTYDGHLKYRLLWQTEGSNKMPLSLVLFSTMSVNTLRFPNLTYERTFVTRLN
                     YVHQMIIGRKFNTALSAEILPTIVHRNFVLTSAEKNTVYAIGGAARYKFVQRVAFNAE
                     YFYVPEGQLAPGFTNSLSLGFDIETGGHVFQLHFTNSPIMIDKGFITETTGKWSKGDI
                     HFGFNISRVFTMKQPKKPKE"
     gene            complement(9051..9608)
                     /locus_tag="PK610_01105"
     CDS             complement(9051..9608)
                     /locus_tag="PK610_01105"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRJ34549.1"
                     /translation="MLSRFLLIALFTLPSISEAQTKYFTKKGQISFYSDTPVEKIEAH
                     NKRVTAILEKESGKIEFSVLITAFEFEKALMQEHFNENYMESSKYPKSTFKGNISNIQ
                     SVDFSKDGEYPLTLDGELTMHGVTRKVNEKGKLIISKGKISASATINVALKDYNISVP
                     SVVGAKIAEIIRVEIVIPQFDLLKK"
     gene            complement(9628..10038)
                     /locus_tag="PK610_01110"
     CDS             complement(9628..10038)
                     /locus_tag="PK610_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34550.1"
                     /translation="MKKILFLLFAATLIAGSWYAYREFTRKPGNANSESTDFKLTAEE
                     LRAEFDNEDTANKKLLNKYIEVTGTVESVSPNEGTTDILLSTEDPMTGINIHLISEEN
                     EKVKSVKPGEKITVKGKCNGKLSDIELNAGIILD"
     gene            complement(10040..10408)
                     /locus_tag="PK610_01115"
     CDS             complement(10040..10408)
                     /locus_tag="PK610_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34551.1"
                     /translation="MLRLSLAIFSIGLMIQFTSCVKQNEEDLSGAKYCKIDTPSFQQH
                     IQIIVNDRCMPCHNASNAESGFDLSNYTNIADIATNGVLQGVIRHESGYIPMPASAPK
                     MPFCEIQIIDKWIEQGALNN"
     gene            complement(10410..11222)
                     /locus_tag="PK610_01120"
     CDS             complement(10410..11222)
                     /locus_tag="PK610_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ34552.1"
                     /translation="MKRSFTTLSIIAAFSLVLSAYRDGPAFNNVDRTGSPVAGGQFCS
                     GCHSGGSFGVSIQGQLKDVGGNVVTSYTPGEDYTFVVQLGNTSGTPKFGFQAVALQAG
                     NTNAGNFTPNSSNTQVTTLNNRKYGEQPAASTGNLFVLNWTAPAAGTGNVTFYAAGVA
                     CNGTNTTSGDQGIKMNALVITENLVQAVSENNLLQISIYPNPSSDFLNITAENESIEL
                     YSIYDLTGKQVLQNSIFSSATQIDIRSLQPGTYVLRLNTTKGTYLISRFIKF"
     gene            complement(11353..11682)
                     /locus_tag="PK610_01125"
     CDS             complement(11353..11682)
                     /locus_tag="PK610_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009640951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HRJ34553.1"
                     /translation="MSLKEAQLIVDTWINNHGVRYFNELTNMAMLTEEVGEVARIIAR
                     RYGEQSEKESDKEKDLGDELADVLFVLICLANQTGIDLETALRKNLDKKTERDQDRHK
                     NNPKLDR"
     gene            complement(11760..12929)
                     /locus_tag="PK610_01130"
     CDS             complement(11760..12929)
                     /locus_tag="PK610_01130"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRJ34554.1"
                     /translation="MLFLGDEQLTRLKTISFLIDKEAKIVYASGSIRALLGYTLEEIT
                     GKSWYSFLDESRKKRIEHFRELELHFLNNPEINHFSVEKKMKTKYDKICWMKWNISRD
                     ENGMYLGIGQDISDLKKNQVKLNNVNRKLAHQKKEIQDSIAYAKRLQDAILPEVNGLH
                     QFFADAFVLNQPKDIVSGDFYWFTKKEDWFIFLIADCTGHGVPGALMSILAHSVVRNV
                     IRETKKVHPAAMLAQIDKELVAALNEKENGTIKDGMDIALCAFQPTTGMLRFAGAFRP
                     LIMIRDDQFSELPASRFPIGFFDDVKKEFIESEIQLKTGDQLFLFTDGYVDQFGGAES
                     GHESKKLNKKRFKELLHQCSEMKGDEKESYLSWAHSNWRQHEEQTDDVLVLGVKI"
     gene            complement(13138..13590)
                     /gene="dtd"
                     /locus_tag="PK610_01135"
     CDS             complement(13138..13590)
                     /gene="dtd"
                     /locus_tag="PK610_01135"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRJ34555.1"
                     /translation="MRVVVQRVQRAEVKIGGITKGAIRSGLLILLGIEENDTDEDVVW
                     LCNKIAQMRIFSDSDGKMNLSVMDCKGELLVVSQFTLHASTKKGNRPSFISAARPEKA
                     IPLYESFCTQLKSGSGLTVETGEFGADMQVELINDGPVTIIIDSKARE"
     gene            complement(13592..14524)
                     /gene="rsgA"
                     /locus_tag="PK610_01140"
     CDS             complement(13592..14524)
                     /gene="rsgA"
                     /locus_tag="PK610_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRJ34556.1"
                     /translation="MKGLVTKSTGSWYSVRLDDGADLECRIKGKFRIKGNTSTNPIAV
                     GDRVLIDSDTDGTGIITELEPRRNYIIRKSVNLSRQGHIIASNIDQAVVFITLKQPDT
                     PLGFVDRFLVTAEAYHIPCLLLFNKSDLLDADEKMIAGELMNEYEQIGYHCMLFSTQN
                     DDIQPLRELFHNKVTLISGQSGAGKTSFINALEPGLQLRTGSISDYHGKGMHTTTFAE
                     MFPLENGGWIIDTPGIKGFGLIDMEKGELADYFPEIRALKAQCKFSNCQHLNEPGCAV
                     KKAVDEGSIWENRYMNYLSIRNDDEDETYRGKGY"
     gene            14600..15517
                     /gene="gldA"
                     /locus_tag="PK610_01145"
     CDS             14600..15517
                     /gene="gldA"
                     /locus_tag="PK610_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013187231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HRJ34557.1"
                     /translation="MSISVKNITKLYGEQRALDEVSFEIGANEVVGFLGPNGAGKSTM
                     MKILTCYIPPDSGRAMVCGLDVMEEPLKVKEKVGYLPEHNPLYLEMYVKEYLHFIAGL
                     HKLDNKDARVKEMIGMVGLDPEQNKKIGALSKGYRQRVGIAQAMIHDPQVLILDEPTT
                     GLDPNQLEDIRKLIVEIGKTKSVMLSTHIMQEVEAICSRVVIIDKGKIVADNSTRNLQ
                     QDSTQRQVVFVEFDKAVSRSQVSSIPGVSNAKNVEGNNWIIESKQEQDIRPVISRFAL
                     DNGLLVLTIRKEEVTLEDAFKRLTGKRNA"
     gene            complement(15539..>16441)
                     /locus_tag="PK610_01150"
     CDS             complement(15539..>16441)
                     /locus_tag="PK610_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34558.1"
                     /translation="QPHIIEFLFGFRKGFFIYAPLFVLFIPALLLGWKEHRYKMWIAA
                     FFILITAYIFSSWYNWFYADLFAMRPMIDFSGAFILAGLILLFRNGFHRLRAGFVVVF
                     SIISIAFNLLFSYQFHHGIIHPNGMNAGKFLSVFLKTGERYHHALGGSFDLPPYAPKG
                     LDTLAEWKACFENESNDYRERAKEEFPLGKDIEINDHWVNAVKLWVQAKFKIEFPNTE
                     AGKNFVLVIQLINSKKEVKYYFPIPLREYINEESGKFLDRSICLSIYNTAEKGDTYSA
                     YIWNRDQEMIVLKDFELYWLSPKK"
BASE COUNT         4768 a         3260 c         3606 g         4707 t
ORIGIN      
        1 gaagaaaaat tcaataagct aatggtgttt tacaaagaag gaattaaaca aaccggatgg
       61 aacagatacg acaccattaa tttaaaatac aggaatcaaa tcgtttgctc aaaaaaataa
      121 accaacaata gaaacaaaca acaaacaagt ataatcatgg aatcacctga gatcatcgtc
      181 ggactcgaca tcggaaccac caagatcgcc tgcctggtgg gacgcaaaaa tgaacatgga
      241 aagatcgaga tcctcggcgt aggaaaatcg gaatcggtag gcgtaacgcg cggtgttgtt
      301 tccaacatcg aaaaaacggt tgcatcaata aaagcagctg tagaagaagc ctcacgtcaa
      361 tcgggagtgg agattaatgt tgtaaacgtc ggcattgccg gtcagcacat caaaagtctt
      421 cagcaccgtg gtattcgtac gcgaagcacc attgaggagg agattgcgca gaacgatatc
      481 gatgcactga tcgacgacat gtacaaattg gtaatgttgc cgggtgagga gattattcat
      541 gttttgccac aggagtatat tgtcgataac gaacagggaa tcaaagatcc gatcggtatg
      601 tcgggaattc gtctggaggc aaatttccac atcatcaccg gacaaatcgc tgctgcaaaa
      661 aacattcata aatgtgttgc caaagcagga ttggaagttg atgaattgat tcttgaacca
      721 cttgcttcga gtgaagctgt tttgagcaca gaagaaaaag aagccggtgt tgtattggtg
      781 gatataggtg gtggaacaac ggatattgcc attttccagg atggaattat tcgtcatact
      841 gctgtaattc cttttggcgg aaacgtgatc accgaagacg ttaaggaagg atgcaccatc
      901 attcacaaac aggcagagct tttgaaagta aaattcggaa gcgcattggc aagtgaagca
      961 caggccaatg aaattgtttg tattcccggt ttacgcggac gtgatccaaa agaaatttca
     1021 gtacgcaatt tggcttctat cattcaggcg agaatggaag agatcgttga gcatgtgtat
     1081 tatgaaatta aaaattctgg atacgagaag aaactaatcg gtggaatagt ggtaaccggt
     1141 ggcggatcgc aattgaagca tatcacccaa ttgtttgaat acatcacagg aatggatgcg
     1201 cgaattggat acccgaccga acatctcgga aaaggtgcag aaggtgttac cagtccgatg
     1261 ttctcaaccg gtgttggact tgtactaatg ggtttccaga aatacgagaa acaagcccgc
     1321 agcaaaaacg cggttatcaa aacgcactcg caaaaaacga aaggttcatt cttcgaaaag
     1381 attttcggaa aagtaggtaa atcgtttttc gaagaggata tcgattaaat actcaaggca
     1441 aacaaacaaa caacaataaa caaacaacaa aaacccaacg ctatgttcaa gtttgaatta
     1501 cccagagaac aagcatcgat cattaaggtg atcggtgtgg gcggaggcgg tagtaatgcc
     1561 gtgaattata tgttccgcca tcagattaag ggtgtcgact ttatcgtctg caatacagac
     1621 agtcagtcac tcgaaatgag tcctatccca actaaaattc aattgggagc gagtcttaca
     1681 caaggactcg gagcaggtgc gattcctgaa gtgggtcgca acgctgcaat tgaaaacatt
     1741 gaagacatca aggccattct cggtcagaat acccgcatgg tattcattac tgcaggtatg
     1801 ggtggaggta ccggaacagg tgctgcaccg gtaattgccc aggctgcccg cgaaatggga
     1861 attcttactg taggaattgt taccattccg ttcggtttcg aagggaaaaa acggaaacaa
     1921 caagccgaag aaggtttgga attcatgcgt caggccgttg atacattgct tgtgatcaac
     1981 aacgacaaat tgcgtgagat gtacggtaat cttacacttg caaatgcgtt ttcacaggca
     2041 gatgaagtgt tgaatactgc tgctcgcgga atcgcagaaa tgatttccgt aacaggatta
     2101 atcaatgtcg atttcaatga tgtgaacacc gttatgcgta acagcggtgt tgccattatg
     2161 ggatctgctt ctgctgcagg agaaaatcgt gcgcgcattg ctgttgaaaa ggcattgtct
     2221 tcaccattgc taaacgaaaa tcatatttac ggtgcacaat acgtgcttct gaatattaca
     2281 tacggtacgc aggaagtcac catggatgag attgctgaaa tcactgatta tattcaggaa
     2341 gaagcaggat ctactgcaga tgtaatctgg ggtcatggat ttgatgaaat gttaggcgat
     2401 tctgtttctg taacagttat tgcaaccgga tttaatcgca ctcctgatac aggagttgct
     2461 atgaaaatgc cggaaaaaaa caaagtcatt cttaccgagg aaacaccaac cgcaatcact
     2521 gcgcctattc agtcacccac gcagcgtcag acggtttctg aatcggttcc tgagccctat
     2581 ttgaaaggtg gcgtggaaga agtacatgct gagatttctg aatcgtcagt tgcactggat
     2641 gaaccggttt tgaaaattat ggatccggta aatgtaactc ctgttgcaga aaatacagtg
     2701 agtgaaacac cgataacccg tcacacactc gataccacat ctatcgaaga gatcacaagt
     2761 tctattgagg aaaatgtggt ttttacgtct gaaaagaatg ctgatctacc agtttccgaa
     2821 aatcctgaat gggaaatcac ctcatccgtt gaagaagaaa cgcttcaatt tgaagtacgc
     2881 accgaagcac caaaaaccan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     3001 ccgttgaaga agaaacgctt caatttgaag tacgcaccga agcaccaaaa accattgatg
     3061 taagtgcaga aactccacag gagaatgtca ttcgccactg gctcgatgaa agtgaagatg
     3121 taaacgatct gaaggcgaaa ggtccggaaa tgaattccca gcaaaacaca cctgaaacaa
     3181 ccggcgagaa taagcaacag gatgtgcgaa gtgagattcc ttccacaaag ggacaaagca
     3241 ccttgtttcc agttgagaat gttgatcctg aaaaggaaaa gcaattgagt tcggaagagc
     3301 agcagcgcag agctcaggaa cgtttggcac gcatcaagga acttagttcc aaattgaaat
     3361 cgcctgccgg actaaacgat ctggaaaacg aaccagcgta catccgtcgc aaaatcacat
     3421 tggacaaaac ccagcattca tccgagtcaa acatctcccg tttttcggta ggtggtgatg
     3481 gcaaagatga aagcggaaac ggtggcggat tgcgatccaa taattccttt cttcacgata
     3541 acgttgactg atatgaatct gagtgagcaa atcaatgccg acatcaaatc agcgatgctg
     3601 gcaaaagaaa aagaaaaact ggaagcgttg cgtgcaatta aagcagcatt acttcttgag
     3661 caaaccaaag gaaccggtga ggtttccaat gatgctgaag tgaaaatgct gcagaaaatg
     3721 tataagcaac gcaatgaagc agcagagatt tttgttcagc agggtcgccc tgaactcgcc
     3781 gaaacggaaa tctttcaggc gaatatcata aaagcctatt tgcccgaaca gatgagtgaa
     3841 gaacaggtaa aagctgtaat tcaggaagtc attgcctctg ttggcgcaag ctctgcagcg
     3901 gatatgggta aggttatggg tcctgccatg gccaagctcc agggtaaagc cgatggtaag
     3961 ctcatttctg catttgttaa gcagttattg ggataaattt tgttttgttt tgttgtttgt
     4021 taacgaagcc cccgctcttg tgcgggggtt tttgtttttg ggaagtagtg tgtatttcag
     4081 agtattaccg gtctacaatc gcattccttc tgaattcagg aattgaaagt gggatcggtt
     4141 gtttagggta atggactttt ttttacattt agtagctatg atcagaacac tcttttttgt
     4201 ttttgcgctt gcatttattg cagcctgcag aagagatgtc cctattccgg atgatattcc
     4261 ggatgaggat accggagttg tgtttaatct cgatcaggtt ccttatccta agttaagcga
     4321 ttataaattt tttacgggtg cattaaaaaa tttgaacccg aacgaaagag tgttgcctta
     4381 cgaacccatt acaccgctct tttctgatta tgcgcataaa tcacgatttg tgtggatgat
     4441 gaaagatagc agcgcacaat atgtttccga tcatagtcta ttcgattttc cggatggaac
     4501 agtgttcatt aaaaacttct attacgataa tgttgttcca tccggcgagc gaaaaatttt
     4561 ggagacaaga cttctttaca aaaaagaagg agaatggaaa tttgcagatt acatctggaa
     4621 tcaggatcag tcggaagctg tttatgaaac taatggaagt tatgttcctg ttcaatggat
     4681 cgacgatcaa ggcgataatc aatcattgac atttcgtatt ccttcggaag ttgagtgttt
     4741 cacttgtcat aaatccaatg ggaataaaat tccgattgcg ccaaaacctc agaacctcga
     4801 taaggatttt tcgtatccag atggcgtaaa aaatcagctt gtaaaatggc aggaagtcgg
     4861 ataccttaca ggaaatgttc cgggtacaat cgcgcgtgta gcagattgga ctgacgacac
     4921 tcagccgctt gaaacacgag ttcgttctta tttggatatt aattgtggat tttgccatag
     4981 tgatggtgga cattgcaatt accggccaat tcggcttgag tttttcaaaa cacttgatcc
     5041 ggctatgttg ggagtttgtg aaattccgca acagtttatt actccccagc tcacccatgt
     5101 aattgcgcgt tcaaacattg cacgttctgt tatgcatttc cgaatgagca gtactgatcc
     5161 gcaatacatg atgccattgc ttggacgtag tgttgtgcat caggaagcag tcgatctcct
     5221 tggtcaatat attttatcac tcactccgat ttgtaattga gcacctccaa accagaaaat
     5281 aaattttaca gttatgcaaa aaagaattct acttagtatt atgacattgt tcagtgcaat
     5341 gcatctctat gcgcaacaaa gttgtgccac ggctatccct gctgttttag gaacaacaac
     5401 tgcttctacc tcttttggaa caacacctcc ggcacctgcg cattgcatgg tgttaaatgg
     5461 atctgaatct tttctgtggt acacgttcac accggcagtc gatacgaacg taagagtaag
     5521 cacagttact tctaccaata ccggattaga tacacgtatt tcggttttca gcggaacatg
     5581 tggcgctcta agctgtattg cttatggtga tgatgaatct gcgtttttat ccattgcaac
     5641 atttgcagcg actgcaggaa caacgtatta tattgttttt gatacgtatt ggggaaacat
     5701 gccgattgat tttaccattt catacgtttc aaccggtggt ggtggcggtc ccattccgca
     5761 attgatcact ttcaccactc agggaattcc caatggcgat aatagtcgtt gtgtaatgga
     5821 tatgaatgga gatcatattg atgatattgt ctatacatca ggaaacacat tgagggtgca
     5881 ttaccagaca gctcctgcaa cgttcacttc aactacgcat accatttcag gcacagttac
     5941 caatgcgcca tcctggagta ttgcaggtgg tgatatggat aacaacggat acaatgatct
     6001 aatttacggt ggtggaagtg gtgctagtat tattatggcg aactctaccg gaacagctta
     6061 cacgcattat tcgggaggac aatacatttt ctcacagcgc accaatgtag tcgacattaa
     6121 caacgatgga attgcggatg cgtttgtctg tcatgatatt gatgcgaatg ttttttatat
     6181 cagtaacgga tcaggaggat tcacctggaa ccagggcgga ctcggtgcaa atggaggaaa
     6241 ttacggttca gtttggatcg attacgataa cgattgcgac atggatctat acatagccaa
     6301 atgtggaagt gatccgatcg atcagcttca cagaaacaac ggtgatggca catacaccag
     6361 tgttggcgtc gctgcaggat tgaatgatgc aaccgagaac tggtcttcta catgggcaga
     6421 ttttgataat gacggcgaca tggatgtatt tatgggcgca tcaagcaact ggggaggatt
     6481 aagtcataaa ctctaccgca acaacggtaa cggaacattt acaaatatta cagccggttc
     6541 aggttttgat ttgtattaca gtaatcttgg aatagaaaac tatccaggtg atttcaataa
     6601 cgatggttgg gttgatgtat atggtttggg tggtttgatt atgtttaatg atggcgacat
     6661 gacatttacc gcatcaactt caccggtggc aggagctgcg gttggagacc tcaatcagga
     6721 tggtgcgctt gaccttgttg cgggcacaac tatttacaaa aacaatttaa cgtccaacaa
     6781 ttacatcgtc gtttcaactg ttggagtgca gagcaataaa aacggaattg gcgcacgtgt
     6841 tacagtgcaa accgcaagtg gctctcagat tcgtgatgtg agaagtggtc aggcttttag
     6901 ctcaatgcaa acgctcaatg ctcatttcgg aattggtcag gaaactgcta tcgaaaaaat
     6961 caccatttgc tggcctagcg gtgtagtgga tgtgataaac aatcctgcta ttaattccca
     7021 ccttgtggtt gtagaaggca gcactgtttc cgtgcaggaa gagttatcac aaaacctgca
     7081 ggtatttcca aatcctgcaa cgagccattt aagcattagc ggattgcccg caaacagggt
     7141 atttgctctt gagattttcg atgttacagg aaaactgatc agcacgcaac aagtttctga
     7201 gaatgttact gtagatgtgc aatcacttgt tccgggaatc tatgttgccc gcatttcagg
     7261 tgaggcatcg caattgaatg tcaagttcat taaagaataa ttcctgcttg acggaaatgc
     7321 agtattaagt gatgcatttc tgttaaattt ggacatgact aattttcaaa acaaagttgt
     7381 ctggataacc ggtgcttcat ccggaatagg tgaggagttg gcttatgcct tttccgggaa
     7441 tggtgcgcgg ctggttttgt ctgcacggcg ttcatccgag ttggagcgcg tacgcaatgc
     7501 ctgtgcaaat cctgaaaatg tttttatcct tcccctggat cttgaaaagc aggaagaatt
     7561 tcctgtattg gtaaataaag tcgttttgca atttggcaga atcgattttc ttgtaaacaa
     7621 tggtgggatt tctcagcgtg caaacgcatc tgaaacgcca atggaagttg acaggaaaat
     7681 tatggaagtc aattattttg gaagtattgc ccttacaaaa gctgtgttgc cacaaatgaa
     7741 acaacagggc agcggaacaa tcattgtaat gagcagtatt gccgggaaat tcggttttta
     7801 tttacgttct gcctattccg cagccaaaca tgcattgcat ggtttttatg aatcgttgcg
     7861 attggaagag gagaagaacg gaattcgcat caccattgtt tgtcccggaa agatcaatac
     7921 aaatatttcg ctcaatgcac taagtaagga tggcaatgcc catggtgtaa tggatcacaa
     7981 ccaggagaca ggcatgcctg ctgatgaatg tgcgaaagta attttaagag gtgtcagcgc
     8041 cggaaaagag gagctttttg ttgggggaaa agaactaaaa gcagtaacgc tcaaacggtt
     8101 tttcccgagg ctttttggaa aaattatcag gaaacagaaa gcaacctgat cgggttctaa
     8161 attctactct tttggttttt tgggttgttt cattgtgaaa acccttgaga tattaaatcc
     8221 gaagtggata tcacctttgc tccattttcc tgtagtttca gtgatgaatc ccttgtcaat
     8281 cattataggg gaattggtga aatgcaactg aaatacgtgt ccaccggttt caatatcgaa
     8341 tccaagtgaa agcgaatttg taaatccagg agcaagttgt ccttcgggaa cataaaaata
     8401 ttctgcatta aaagccacac gctgaacaaa cttataacgt gctgcaccac caatggcata
     8461 aaccgtattt ttttcagcag aagttagcac aaagtttctg tgtactatcg ttggaagaat
     8521 ttctgcactg agagcggtgt tgaattttct gccaataatc atttgatgaa catagttcag
     8581 tcgggtaaca aaagttcttt cgtaagtaag attaggaaag cgaagggtat taaccgacat
     8641 ggttgagaac aacaccagac tcaatggcat tttattgctg ccttctgttt gccacagtaa
     8701 ccggtatttt aaatgaccat catacgtctt ttcataggtg cttcttccaa caccaatctg
     8761 aaatcggtcg gtaataccat aatcgaaact taaacgtagg gaagcctgat caagtccgaa
     8821 taactcatac aatccgccat tgagtcggcc aaagcggtgt gaaatacgaa aatccagcac
     8881 tccggcatgt gtggtttcaa gcgattgagc gttgatgact tttgggtttt taaaacttgc
     8941 tcttacatgt tgaagttcat catctccttc aatttcattt agtaaatcct gggcattcaa
     9001 ttcaggaata caaattaaat gcagagcgat aaaagagaga cagaatagtt tcatttttta
     9061 agtaggtcaa attgaggaat gacaatttca acgcgaatga tttcggctat ttttgcacct
     9121 acaacggaag ggacagaaat attgtaatcc tttaatgcta cattgatcgt tgcagaggcc
     9181 gaaatttttc ctttcgaaat gatcaatttt cctttttcat ttacttttcg ggttactccg
     9241 tgcatggtta attcgccgtc aagtgttagc gggtattcac cgtctttgga aaagtcgacg
     9301 gattgaatat tcgaaatatt tcccttgaat gttgatttag ggtacttcga cgattccata
     9361 taattttcgt tgaagtgttc ctgcatcaat gctttttcaa attcaaatgc agtgatcaga
     9421 actgaaaatt caattttacc gctttctttt tccagaattg cagtaacacg tttattgtgc
     9481 gcctcaattt tttcgacagg ggtatcggaa taaaaggaga tttgtccctt cttggtgaag
     9541 tactttgttt gtgcttcgct tatagagggt agtgtgaata gcgcaattag taaaaatcgg
     9601 gatagcataa aataggtttt agagggatca atcaagaata atgcctgcgt taagttcaat
     9661 atccgataat ttaccgttac attttccttt aactgtaatt ttttcaccgg gctttaccga
     9721 tttaactttt tcattttcct ctgagatcag gtggatattg attcctgtca tgggatcttc
     9781 tgtggaaagg aggatatcgg tagttccttc attgggagaa accgattcaa cagttccggt
     9841 tacctcaatg tatttgttta acagcttttt gttggcggta tcttcattat caaattctgc
     9901 gcgtaattct tcagccgtta atttgaaatc tgttgattcg gaattggcat taccgggttt
     9961 cctggtgaat tcacggtaag cgtaccacga tcctgcaatt aatgttgctg caaaaagaag
    10021 aaacagaatt tttttcatgc tagttattta gagcgccttg ttcaatccat ttatcaatga
    10081 tctgaatttc acaaaatggc atttttggtg cgctggcagg catggggatg tacccggatt
    10141 catgccggat tactccttgt aaaactccgt ttgttgcaat atcagctatg ttggtataat
    10201 tgcttaggtc aaatccgctt tcggcattgg aagcattatg gcaaggcatg caacgatcgt
    10261 tgacaatgat ctgaatgtgt tgttggaagg aaggagtgtc aattttgcaa tattttgctc
    10321 cgctcagatc ctcctcattc tgtttcacac aggaagtaaa ttgaatcatt aatccaatac
    10381 tgaaaattgc aagggaaagt ctgagcatat taaaatttaa tgaatcggga aatcaggtaa
    10441 gttccctttg ttgtattcag acgcaaaaca taggttcccg gttgcaagct ccggatatcg
    10501 atttgtgttg cacttgaaaa tattgaattt tgtaacactt gttttcctgt aagatcataa
    10561 atggaataca actcaatgga ttcattttct gctgtgatat tcaggaaatc ggaggagggg
    10621 ttgggataaa ttgaaatttg caggaggttg ttttcagata ctgcctgaac aagattttca
    10681 gtaatcacca aagcattcat tttaatgcct tgatctccgg aggtagtatt ggtgccgttg
    10741 catgcaactc ctgctgcata aaaagtaaca tttccggttc ctgctgcagg tgctgtccag
    10801 tttaaaacaa ataaattacc ggtacttgct gcgggttgtt cgccgtattt gcgattgttc
    10861 aatgtagtta cctgggtatt gctgctgttt ggtgtgaaat tccctgcatt ggtatttccg
    10921 gcttgcagcg ccactgcctg aaacccaaac ttcggtgtgc ctgaggtatt gcccaactga
    10981 actacaaaag tataatcctc tcccggtgta tagcttgtta ccacatttcc acccacatcc
    11041 ttaagttgtc cctgaatact tacaccaaag cttccgcccg aatgacaacc tgaacaaaat
    11101 tgtcctcctg caacaggtga accggttcgg tctacgttgt taaatgccgg accatcccta
    11161 tatgccgaaa gtaccaggga aaaagcagcg attatcgaga gggtagtaaa actgcgtttc
    11221 atagcttttg cttattcaaa tataacaata gccattgata aatgttgttt atacaactaa
    11281 acaaaaagga aacggctgaa aatgaaggtc aatcggttgt ttgaggtcga gtcttgttta
    11341 ttgttgaaag gatcagcgat caagttttgg attgtttttg tgccgatcct ggtcacgttc
    11401 tgtttttttg tcaagattct tgcgcaaggc tgtctcaaga tcgattcctg tttgattggc
    11461 cagacaaatg agtacaaaca aaacatccgc cagctcatcg ccaagatctt tttctttgtc
    11521 gctttccttt tccgattgtt caccgtatcg ccgtgcaatg atgcgggcaa cttctcccac
    11581 ttcttcggtt agcattgcca tattggtgag ttcgttaaaa tagcgaacac catggttgtt
    11641 gatccaggtg tccactatca attgtgcttc ctttagactc aattcactca tgttcaaata
    11701 ttgcaaattt tacggatata acagtgagaa attcggtgaa gatcagaaat agaatgagtt
    11761 caaatcttta ctcccaaaac caatacatcg tctgtttgtt cttcatgttg tctccagttc
    11821 gaatgcgccc aactcaaata cgattctttt tcatcgcctt tcatttcact gcattgatgc
    11881 aataattcct tgaagcgttt cttgtttaac tttttactct cgtgtcccga ttcggctccg
    11941 ccaaactgat caacataacc gtcagtaaac agaaacaact gatctcctgt tttcagttga
    12001 atttctgatt cgatgaattc ttttttcaca tcatcaaaaa agccaatcgg aaaacgcgag
    12061 gccggaagtt cagaaaattg gtcatctctg atcatgatca atggtcggaa tgcacccgca
    12121 aatcgcaaca tgccggttgt tggctggaat gcgcataatg cgatatccat tccgtccttt
    12181 attgttccat tttctttttc attcaatgcc gccaccagtt ccttgtcaat ctgagcaagc
    12241 attgctgcag gatgaacttt tttcgtttcg cggattacat ttctcactac cgaatgcgca
    12301 agaatcgaca tcaacgctcc gggaactccg tgtccggtac aatctgcaat aaggaaaatg
    12361 aaccaatctt cttttttggt gaaccagtag aaatcaccgc tcacaatgtc tttgggttga
    12421 ttcaaaacaa aagcatccgc aaaaaactga tgcaatccgt ttacctccgg gagaatagca
    12481 tcctgcaatc gtttggcata ggcaatgctg tcctgaatct ctttcttttg atgtgcgagt
    12541 ttgcggttga cattattcag tttgacctga ttttttttca gatcgctgat gtcctgacca
    12601 atcccaagat acattccgtt ctcatcacgg gaaatattcc atttcatcca gcagatctta
    12661 tcgtatttgg ttttcatttt tttctcaacg gagaaatggt tgatctccgg attgttcaaa
    12721 aaatgcaatt ccaattcacg aaaatgttca atcctttttt ttcttgattc atccagaaaa
    12781 gaataccagc tttttccggt tatttcctcc agggtatatc ccaaaagtgc acggattgaa
    12841 ccggatgcat aaacaatttt cgcctcctta tcaattagga acgaaatggt ttttaaacgg
    12901 gtaagctgct cgtcgcccag gaatagcata aaacataaat taaaaatgaa attgaatagg
    12961 gagaatggag gacctgaatc aggccataat tcggccaaac ggtgctggac gcagtgtgcg
    13021 tctaaaatta gtccaatatg ttccgtttag gatcatgcga caaatgaaag aagatttttc
    13081 attgtgacat tgaatcggaa caacagaaaa aatgcatttg ccgtatccga ttcaggatca
    13141 ctcccgtgct tttgaatcga tgatgatggt aaccgggcca tcattaatca actctacctg
    13201 catatctgcg ccgaactcac ctgtttcaac ggttaatcct gaaccgcttt ttaattgagt
    13261 gcagaatgat tcgtaaagtg gaatcgcttt ttcgggacgt gctgcggaaa taaatgaggg
    13321 acgatttcct ttttttgtgg aagcatgaag tgtgaattgc gaaacaacca gtagttctcc
    13381 cttgcaatcc ataacagaaa gattcatttt tccgtcgctg tccgaaaaga tccgcatttg
    13441 agcgatcttg ttgcataacc atacaacatc ttcatctgtg tcattctctt caatgccgag
    13501 aaggataaga agtcctgaac gaattgctcc ttttgtaatt ccgccgattt ttacttcggc
    13561 acgttgtaca cgttgcacta caactctcat ttcaatatcc ttttccgcgg taggtctcgt
    13621 cttcgtcatc attacgaatc gagaggtaat tcatgtatcg gttctcccag atcgatcctt
    13681 catccaccgc tttttttacc gcgcaacccg gttcgttgag atgctgacaa ttactgaatt
    13741 tgcattgtgc ttttaatgca cggatctcag gaaaataatc agccagctcg cctttttcca
    13801 tatcgatcag accaaatcct tttattccgg gtgtgtcgat aatccatcct ccgttttcga
    13861 gaggaaacat ttctgcaaag gtggtagtgt gcatcccttt tccatggtaa tctgaaatgg
    13921 atccggtacg taattgaagt ccgggttcaa gggcattgat aaaacttgtt ttacctgcac
    13981 ccgactgacc tgagatcaga gtcaccttgt tatgaaacag ttcacgaagt ggttgtatat
    14041 cgtcgttctg agtagagaac agcatgcagt ggtaacctat ctgttcgtat tcgttcatca
    14101 gttcaccggc aatcattttt tcatcggcat cgagcaaatc acttttgtta aacaagagca
    14161 aacaaggaat gtgataagct tctgcagtaa ccagaaaccg atcaacaaat cccaatggag
    14221 tatcgggctg ttttaacgtg ataaaaacaa cagcctggtc gatgttggag gcaataatat
    14281 gaccttgtcg ggaaaggttt acacttttgc ggatgatgta gttgcgcctt ggttcaagct
    14341 cggtaataat gccggttccg tcggtatcgc tgtcgattag aacccggtcg cctaccgcaa
    14401 ttggattggt ggaggtattc cctttgatcc tgaatttgcc tttgatcctg cattccagat
    14461 ccgctccgtc atcaagacga accgaatacc agcttccggt tgatttagta acaaggcctt
    14521 tcatggctta aagttaaggg taaaagttca tcctgccttg cttctgtctt tcaatgatca
    14581 aaatccttac ctttacacca tgtccatttc ggtaaaaaat atcaccaaac tttacgggga
    14641 gcagcgcgct ctggatgagg tttcatttga gataggtgcc aatgaggtag ttggttttct
    14701 tggtcctaat ggcgccggta aatcgaccat gatgaaaatt cttacctgct acatcccgcc
    14761 ggattccgga agggctatgg tatgtggttt ggatgtgatg gaggaaccgc tgaaagtgaa
    14821 agaaaaagtt ggctatttac ccgaacacaa tccgctctat ctggaaatgt atgtgaaaga
    14881 atatcttcac ttcattgctg gattacacaa gcttgacaac aaagatgcga gagtaaagga
    14941 aatgatcgga atggttggat tggatcctga acaaaataaa aaaataggcg cattgtcgaa
    15001 aggttatcgt caacgcgtgg gtattgcaca ggctatgatc catgatccgc aggttttgat
    15061 tttagatgaa ccaacaaccg gacttgatcc caatcagctg gaggatatcc gtaaactgat
    15121 tgttgaaatc ggaaaaacca aatcggtgat gctttctacc cacatcatgc aggaagttga
    15181 agctatctgc tccagggttg taatcatcga taagggaaaa attgtagctg ataattctac
    15241 ccgaaacctg caacaggatt ctacacagcg acaagttgtt tttgttgagt ttgataaagc
    15301 tgtttcacga agtcaggtct catcaattcc gggtgtttcc aatgcgaaaa atgtggaggg
    15361 taataattgg atcatcgaat cgaaacagga gcaggatatt cgtcctgtaa tttcccgatt
    15421 tgcacttgat aacggattac ttgttctcac cattcgcaag gaagaagtaa cactggaaga
    15481 tgcattcaaa cgattaacag gaaaaagaaa cgcctgatag tttgttgcgc tttaaagttt
    15541 attttttcgg acttaaccaa tatagttcga aatccttcaa tacaatcatc tcctgatctc
    15601 tgttccagat ataggccgaa taggtatctc ctttctctgc ggtattgtat atgcttaagc
    15661 atatactcct atcaagaaat ttaccgcttt cctcgttgat gtattcccga aggggaatgg
    15721 ggaaataata cttcacttcc tttttactgt ttatcaattg gataaccaat acgaagtttt
    15781 ttcctgcttc ggtattcggg aattcaattt taaattttgc ttgtacccaa agcttaacgg
    15841 cattcaccca atggtcattg atttcaatat cttttcccaa tggaaattct tcctttgccc
    15901 gctcacggta atcgttggat tcattttcaa aacatgcttt ccattcagcc agtgtgtcta
    15961 atcctttggg cgcataagga ggaagatcaa agctgcctcc caatgcatga tgatagcgtt
    16021 caccggtttt cagaaacaca gaaaggaatt tcccggcatt cattccgttg gggtgaatga
    16081 ttccgtgatg aaactggtag gaaaacaaaa gattgaacgc aatggaaatg atggagaaaa
    16141 caactacaaa tcccgcccga agacgatgaa atccattgcg aaataataat atgagtcctg
    16201 caagtatgaa cgcgccggaa aaatcgatca tcggacgcat ggcaaagagg tcggcataaa
    16261 accaattgta ccaggaagaa aaaatatatg cagtgatcaa tatgaagaat gcagctatcc
    16321 acattttgta gcggtgttct ttccatccta acagcaatgc aggaatgaac aaaacgaata
    16381 gcggtgcgta tatgaaaaat ccctttcgaa atccgaagag gaattcaatg atgtgcggtt
    16441 g
//
LOCUS       DAOMYG010000015        14949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1360_length_14949_cov_9.306432, whole genome shotgun sequence.
ACCESSION   DAOMYG010000015 DAOMYG010000000
VERSION     DAOMYG010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 14949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14949
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1360_length_14949_cov_9.306432"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PK610_01155"
     CDS             <1..950
                     /locus_tag="PK610_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34559.1"
                     /translation="VFGSAEVSVSDFQFHGDNFVMLVYEKRIDKGSKVIYTSADGTLL
                     SSFTVPDIAKELYKDFAGRIYLICVSKVYEVTIRDAMIRLLPVEADHFDRQIRPWVDT
                     TESKAYFSNYLWYYPEFDYFSWSAKDSGFKKIRTVIDKPLMELYCAQYKYVSGREKLE
                     AYRAQKATGVDKEIWIAIWSGFPNSLYYHPLYAPMFVQNDTILIFDHYTNKLFRYNAE
                     NEPIDSLDISYHIGPDKKEWEELLVKDVEEQVIYAVYLKGGKYYLKELNTSTGEVKNV
                     YKLTYKYPERLKIRGGYAYYIYRPFESAQKKFLYKEKIR"
     gene            1013..2020
                     /gene="bioB"
                     /locus_tag="PK610_01160"
     CDS             1013..2020
                     /gene="bioB"
                     /locus_tag="PK610_01160"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HRJ34560.1"
                     /translation="MSDIRHNWTKEEILAIYNKPLMELLYEAATMHRKHHDPSVVQVS
                     SLISIKTGGCPEDCAYCPQAARYNTKVKVHKLMDVDEVTSLAKNAKANGASRVCMGAA
                     WRNVRDNKDFDKVVDMVKSVHALDLEVCCTLGMVTELQAKRLADAGLYAYNHNLDTSE
                     EYYDDIISTRTYDERLKTLENIRNAKLTVCSGGIIGLGESTEDRVGMLQTLANLPEHP
                     QSTPINALVPVEGTPLEEQEIVPIWDMVRMIATTRLVLPKTVVRLSAGRTQMSMEGQA
                     LCFMAGAGSIFAGDKLLTTPNPEFNDDMEMFRILGLQAKKPFADGDKPVVKENAEQSL
                     A"
     gene            2028..3125
                     /locus_tag="PK610_01165"
     CDS             2028..3125
                     /locus_tag="PK610_01165"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HRJ34561.1"
                     /translation="MMPQWLLDRISSALQKRNEEGSLRSLKKSVGLSDFSSNNYLGLA
                     NSPQYGIGSTGSRLISGNSIEAENFERYFAEFTGYETALLYNSGYAANTGVIACLAAR
                     NDTVIYDELVHASIRDGIRLSLAKAYSFRHNDPNDLRKKCEQASGPVFILTESVFSMD
                     GDEAPLKHISDVSREFDAALIVDEAHAFGVFGKEGRGLVNETGIQSLVTACIFTFGKA
                     AGVHGAAVAGDRLLVNYLLNFSRSFIFTTALPPNEILRIESQINRLVQSEKERTQLKA
                     HIEIISGNFKAYGDRYIVSRSPIQCLIVPGVEAVRAFSTALQNDGFDIRPIMSPTVRA
                     GTERVRICLHAYNTSTEIDRLLGKIQSLMNQ"
     gene            3122..3745
                     /gene="bioD"
                     /locus_tag="PK610_01170"
     CDS             3122..3745
                     /gene="bioD"
                     /locus_tag="PK610_01170"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HRJ34562.1"
                     /translation="MRKGVFVSGIGTGVGKTVVSALLCRHTKADYWKPIQAGDLENTD
                     SMKVGEWTSDHGITIHQEQHRLMEARSPHIAAKMEGITLNPEKFQLPETPNPIVVEGA
                     GGILVPINEDGNTMADLIRKFDLPIVLVSRYYLGSINHTLLSINYIKQMGLSLKAMVF
                     TGEEAPGTKSAILAVCSGVEVYEVPEIQLLRKDELKKYSFVFEDFSF"
     gene            3907..7668
                     /locus_tag="PK610_01175"
     CDS             3907..7668
                     /locus_tag="PK610_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ34563.1"
                     /translation="MRKLFTGLFFMSLTAAGFAQDLAMAPPLGTFLSPATGCSLTATE
                     TVTIRIFNAGPTILAGTNIPVSYSINAGAPVNEVITLGANFVNNTTLNYTFTAVADLS
                     VPGSYSIDATVALPGDVVPANNTYSGFVVVNNAASNGGTATGGTNVCITSNSGNITLA
                     GNVGSVLNWEYSIDGGGTWINISNTTTTQSYNNLTVPTQYRANVQNASCPTATSTVAS
                     MTIDPASVGGTVTGGTTPACSGANSGTLTSSGRTGTIVRWEFTINGGATWTPIANTTP
                     TQNYLNLVTTTGYRVVVQSGACATAVSATRTIVVSPPTVAGAVNSNATVCSGTNSGTL
                     TLTGNVGAVVDWQFSTNGGATWTTIANTTTTQNYLNLTQTTMYRARVRSNPCATLIST
                     PATITVVAASIGGTLSSNATVCINSNRDTLRLSGQVGTILNWEFSNDNGVTWNNYAST
                     NDTLIYDNLQASRNYRVIVQAGGCAAVNSSVVAITVDTLTVGGIASGAVTVCANGNAG
                     NVQVTGNNGVINHWEFSDDAGVTWNPIANTTSTQPYTNLSASRRFRVEAQNGVCPVGY
                     STEVEISVDAITVAGSVSASTTVCSGANAGTLNLVGNTGNVLNWELSTDGGLSWITVA
                     NTTTSQNYANISQITQYRANVQNGVCPSAISGFAVLTIDAQAVGGTLYGGATVCETAN
                     SGTLTLVGYNASIVEWQESIDGGVTFTPIANTSPFYNFSNLTTTTEYRVITASGVCPN
                     DISSSTVVIVDAATASGIVSANAIVCAGANSGTLTLNGHTGSVTNWEMSNDNGTTWIT
                     LANNTISQDYLNLMTTTSFRAKVQSGVCPAIGSTPATITVNAQSNGGEVNSSATVCAG
                     QNFGVLNLFGQVGNIIDWEFSTDQGTTWTPIGNTSTTQNYANLVDTTWYRVIVQSGIC
                     PIDSSDYAFINLYPSPAASFNGDTVCQNQPLAFVNTTTNPIGFVTLYSWDFGDGGTSL
                     SANPSHIYSSFGTFTASLFAMNSFGCTDTAMRTILVNNLPVADIFPSGPTSFCDGGSV
                     DLSAPFNINYSYLWNTGDNNFQITADTTFNYIVTVTDITNGCVNTDSEMVTAFPLPVA
                     NAGLDTTVSLGYGVMLNGSGGVTYLWSPSLGLDNPSFPTPMATPTTTTTYILTVTSID
                     GCTDSDSVTVVVNNDFLVTVRNIITPNADGFNDTWIVENIESYPNNTITVFNRNGQKV
                     FETDAYMNDWGGTFGSDPLPDGTYYYVIKFNDSDIVVKGAVNIIRSSSN"
     gene            7682..9088
                     /locus_tag="PK610_01180"
     CDS             7682..9088
                     /locus_tag="PK610_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRJ34564.1"
                     /translation="MKKLLYTSVLAAMVAVNAKAQQLPLFSQYYYNKFLYNPAFTGTE
                     DMGQAYLIHRSQWKDMPGSPVTYALTVDGPVKSNQIGLGMSLFNDQTDIFNRTGLYTS
                     YSYRWKVNEDHHLTFGLSGGVIDNKIDFARVSARDVNDPLLYNENRRKITMDANFGVG
                     YFFKDLQVGFSIPQLLGNKVKYNEGATSVYTVNKRHFVGSVSYSLMISETQQIRFIPN
                     IMTRYAKGSPFQFDFNANFDWKDMVRAGLSYRHGYAVGMNIGAKVNKNLVAGYTYEYV
                     ISPIGAVAGGGHEILLGYRFSGGGRGGDDEQIKRMNENLDRAQMENDSLMRELKKKDQ
                     EHTTDIQYLKEEMEKLKKQQEEGNNGTSGKNHPPIEEINKNIRSEKAADYQNEDGTPL
                     TPGYYVIMGSFKVKENAQKSKKDYSTKPGYNPTVLFNKVRGFYYVNVFYSTDEETALQ
                     IMEVLRTEQPDAWVFKME"
     gene            complement(9136..9762)
                     /locus_tag="PK610_01185"
     CDS             complement(9136..9762)
                     /locus_tag="PK610_01185"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRJ34565.1"
                     /translation="MDFIDRLQRQLTEQLPGEEAHLKMAPYRRSVKAELDGAGKEPRL
                     SAVMALIYLKDNQWHLVLTKRNEYEGTHSAQVSFPGGRKEDSDASLIHTSIRETKEEI
                     GVWVPDTQIIGSLTEVYIPPSNFLVNPVLSWLPEQPAFNPDPREVNYIIEFPVDELTR
                     DELIKETKIKLSTGLNMTVPYFDIKGEIVWGATAVILSEFREILMRIK"
     gene            9822..10580
                     /locus_tag="PK610_01190"
     CDS             9822..10580
                     /locus_tag="PK610_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002679348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRJ34566.1"
                     /translation="MEGFDFLFSVDGVIAFLTLAVLEIVLGIDNLVFISIIVDKAPKE
                     KQRFIRLLGLGLALGFRVVMLMGISFIVGLKAPIFSMGNHEFSWRDLILLGGGLFLIA
                     KSTLEIHHKLKSAGMVEHHKEEKKTSAKNVVLNMITQIVLIDIIFSIDSILTAVGLTE
                     NVTLMISAVVISMIFMMVFSGPVGTFINQNPTIVMLALSFLLMIGMLLVAEAFGQEFD
                     RGYVYFAMGFSLTVELLNLVLLKRLYRSKPSDEN"
     gene            10623..10709
                     /locus_tag="PK610_01195"
     tRNA            10623..10709
                     /locus_tag="PK610_01195"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10657..10659,aa:Leu,seq:cag)
     assembly_gap    10730..10829
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11182..13407)
                     /locus_tag="PK610_01200"
     CDS             complement(11182..13407)
                     /locus_tag="PK610_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRJ34567.1"
                     /translation="MNHVPHIIQDLTLILGVAAITTLLFRFLKQPLVLGYLMAGILVG
                     PHFDLFPTVSELKGIQTWAEIGVLFLLFGLGLEFSFRKLFKVGKTAIITALFGIAFTF
                     FFGYLFGTFLGWKPMDALFMGGILSIASTTIIIRAFDESGEKNQLFASVVMGVLVVED
                     LVAVVLMVILSTISVSRNFEGSEMMFSIFKLIFFLILWVITGVFFLPGLFRRLKRFLS
                     DETLLIVSLALCFLMVILATKAGFSSALGAFIMGSLLAETNSGHRIEHLIQPLKDLFG
                     AIFFVSVGMLIDPAMIAEHLWVIVGASLILIIGKPLFITTGALVSGQTLKVSMRSGMS
                     LSQIGEFSFIIASLGLTLNVTSDFLYPVAVAVSAFTTFTTPYMIRFSKPFYGIVDRGM
                     PSSWRSALEKYSLSLQRVPETSAWKRYLRAYVKNIVIYSVIILAFFLLSVRFLFPKFH
                     YDSFSAILFCSIVLLAASPFLWALAFKRTDKTAYSELWLKPSQRGLLVVMQLFRLLLM
                     LFFMGFFFNLVFTPLVALMVIVVSIFVLFLLRFRIRDFYSRIEQRFISNWSGQENRSK
                     EPDQNLAPWDTHMAVVQIGPNLPFLGKSLEEIRLREEFGINIAMIERGNVNLYIPTRH
                     EKIFPGDQLHIIGTDEQLEKFRNFLNEFEIATGPWANLEKNIQLRHFTLTPNSSLVGK
                     SIRASGLRERTSGIIVGIVRNEVRILNPSSDVVFEEGDELWLVGDDKRIKVLIRENEG
                     G"
     gene            complement(13448..14053)
                     /locus_tag="PK610_01205"
     CDS             complement(13448..14053)
                     /locus_tag="PK610_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HRJ34568.1"
                     /translation="MSTLVLVRHGQSAWNLENRFTGWVDVDITEQGEKEAINAGEKLK
                     SLKFDLAYTSNLLRAQRTLSIILKEIGQENIPVTKNEALNERHYGDLQGANKAETAAK
                     YGDEQVHIWRRSFDIAPPKGESLKDTLDRVLPYFEKEIVPKLKSGKNIIVAAHGNSLR
                     ALIMYLEKLSPEQILQVEIPTGAPKKYELDSNLNILSASFI"
     gene            complement(14206..>14949)
                     /locus_tag="PK610_01210"
     CDS             complement(14206..>14949)
                     /locus_tag="PK610_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HRJ34569.1"
                     /translation="GAKNTKPLIFVGKGVVYDTGGYSLKVGGVMQTMKCDMAGAAAVV
                     GAISAIAKNKLPVYVIGLIPATDNRINSHALVVDDVITMHDGTTVEVQNTDAEGRLIL
                     ADALAYAKKFKPELVIDLATLTGAAAMITGQYGSAVMGNESKYKKELIETGDETYERL
                     AEMPFWREYDDLIKSDIADIRNVGGPTGGAITAGKFLARFTDYNWIHLDIAGPAFLKD
                     AKDYHHKGGSGVGVRLLYHFTKKFVKRRP"
BASE COUNT         4246 a         3091 c         3269 g         4243 t
ORIGIN      
        1 ccgtatttgg ttctgctgag gtaagtgttt ccgattttca gtttcatggc gataattttg
       61 tgatgcttgt ctatgaaaag cgtatcgata aaggcagtaa agtcatctat acatcagccg
      121 acggaaccct actttcttcg ttcacagttc cggatattgc caaggaattg tacaaagatt
      181 ttgcaggacg catttacctg atttgcgtat cgaaagtata tgaggttacc attcgtgatg
      241 cgatgattcg tttgcttccc gttgaagcag atcatttcga cagacaaatt cgtccatggg
      301 tagatacaac ggagagcaag gcctattttt ccaattatct atggtattat cccgaattcg
      361 attatttcag ttggagtgcg aaggatagtg ggtttaaaaa aataagaaca gtgatcgaca
      421 aaccattgat ggaattgtat tgtgcccaat ataaatatgt gtcgggacgc gaaaaactgg
      481 aagcctatcg cgcacaaaaa gctaccggtg ttgataagga aatctggatt gccatttggt
      541 cgggctttcc caattcattg tattaccatc cgctttatgc acccatgttt gtgcagaatg
      601 ataccatttt gatttttgat cattacacca ataaattatt ccgctacaat gccgaaaacg
      661 aacccattga ttcgctggat atcagttatc atattggtcc cgacaaaaag gaatgggaag
      721 aattgctggt gaaagatgtg gaagaacaag taatatatgc ggtctatttg aagggaggta
      781 aatattacct caaggaatta aatacatcta ccggggaagt gaaaaacgtg tacaaactca
      841 cctataaata cccggagcgg ctgaaaatca gagggggata tgcttattat atctatcgtc
      901 cctttgaatc cgcacaaaag aaatttctct acaaagagaa gatccgttaa agctcgggtt
      961 ggggatttgg gcaaagcatt taccgcttat atttgcataa acctacccga agatgagcga
     1021 tatccgacat aactggacca aggaagaaat tcttgctatt tacaacaagc cgttgatgga
     1081 gcttctctat gaagccgcca cgatgcaccg caaacatcac gatccctcag ttgttcaggt
     1141 aagctcattg atttccatta aaaccggagg ctgtcctgag gattgtgcat attgccccca
     1201 ggctgcacgt tacaacacca aggtaaaagt gcataaactg atggatgttg atgaggtgac
     1261 aagtcttgcc aaaaatgcaa aggccaatgg agcttcgcgg gtttgtatgg gtgcagcatg
     1321 gagaaatgtg cgcgacaaca aagattttga taaagttgtg gatatggtga aaagcgtgca
     1381 tgcgcttgat cttgaagtgt gctgtacatt gggaatggtt accgaattac aggcaaaacg
     1441 tttggcagat gcaggtttgt atgcttacaa tcacaatctg gatacttccg aagaatatta
     1501 cgatgatatt atttccacac gcacctatga cgaacgatta aagacgcttg aaaatattcg
     1561 taacgcaaaa cttactgtgt gttcaggtgg aattatcgga ttgggcgaat caactgaaga
     1621 tcgtgttggg atgttgcaaa cactggccaa tcttcctgag catccgcaat caacccctat
     1681 caatgcgctt gtgccggtgg aaggaacacc tttggaagag caggagatcg ttcctatttg
     1741 ggatatggtg cgcatgattg caacaacgcg tttggtgctg ccaaaaaccg tggttcgact
     1801 cagtgcagga agaacacaaa tgagtatgga aggacaagca ttgtgtttta tggcgggtgc
     1861 gggttctatt tttgcaggtg ataaattact taccacaccc aatccggaat tcaatgatga
     1921 tatggagatg ttccggattc ttggtttgca ggcaaaaaaa ccatttgccg acggagataa
     1981 acccgtggta aaagaaaacg cagagcaaag tctcgcctga aagtgctatg atgccgcaat
     2041 ggctgcttga ccgcatttct tctgcattac aaaagcggaa tgaagaagga agtttacgat
     2101 cactaaaaaa atcggtaggt ctgagcgatt tctcctctaa taattacctc ggattagcca
     2161 attctcctca gtatggaata ggatctacag gatcacgtct catcagtgga aattcaattg
     2221 aggctgaaaa ttttgaacgt tattttgcag aattcaccgg ttatgaaacc gcattgcttt
     2281 ataattccgg ttacgctgcc aataccggag ttattgcatg tctcgctgca cgaaatgata
     2341 ctgtgattta cgatgaactg gtgcatgctt ccattcggga tggtattcgc ctgtcgcttg
     2401 caaaagcata ttcgtttcgc cataatgatc caaacgacct ccggaaaaaa tgtgaacagg
     2461 caagcggtcc ggtttttatt ctgaccgaat ctgttttttc tatggatggc gatgaagctc
     2521 cacttaaaca tataagtgat gtttcacgcg aatttgatgc tgcactcata gtggatgaag
     2581 cgcatgcatt tggcgtattc ggtaaagagg gtaggggatt agtgaatgaa accgggattc
     2641 aatcattggt aactgcctgc atttttacct ttggaaaagc tgccggtgta catggtgctg
     2701 ccgttgcagg agatcgctta ttggttaatt acctgctcaa tttttcgcga agttttattt
     2761 ttacaactgc gcttcctccc aatgagatac tcagaataga atctcaaata aaccgactcg
     2821 tacaaagtga aaaggagcga actcaattaa aagcacatat tgaaattatc tccggaaatt
     2881 tcaaggcgta tggagatagg tatatcgttt cacgttcgcc tattcaatgc ctcattgtac
     2941 ccggtgttga agcggtgcgt gcattttcaa ctgcattgca gaatgacgga tttgatattc
     3001 gtccaatcat gagtccaaca gtacgtgcag gtactgaacg ggttcggatt tgtctgcatg
     3061 cttacaatac atcaactgaa attgatcgcc tgctaggaaa aattcaatcc ctgatgaacc
     3121 aatgagaaaa ggtgtttttg tatccggtat tggtactggc gtgggaaaga cggttgtttc
     3181 tgcattgtta tgccgtcata ccaaagctga ttattggaaa ccgatacaag ccggggactt
     3241 ggagaacacc gactcaatga aagttgggga gtggacaagt gatcatggta ttacgattca
     3301 tcaggaacaa caccgcttga tggaggcacg aagtccgcat attgctgcaa aaatggaggg
     3361 gattaccctt aatccggaaa aatttcaatt gcctgaaact ccaaatccaa ttgtggttga
     3421 aggagccggt gggattttgg tacctatcaa tgaggatgga aataccatgg ccgacctcat
     3481 ccgaaagttt gatttgccga tcgtgcttgt cagccggtat tatctgggtt caattaatca
     3541 tacattattg tcaatcaatt acataaaaca aatgggctta agccttaaag caatggtatt
     3601 tacaggagaa gaagccccgg ggacgaagtc tgcaattctg gcagtttgtt caggtgttga
     3661 ggtgtatgaa gttcctgaaa ttcaattatt aaggaaggat gagttgaaga aatattcctt
     3721 tgttttcgag gatttttcct tttaaggtaa aaaacgaggt tttcatcatt aattcgcacc
     3781 ttttcaacct attatcaggc ttcttttcaa ttgacctgcg taaatatctt tgtcgaaaat
     3841 cgtcgacatg ttattaacaa ttcattttta attgttaatt tcgtgtatct caatcaacat
     3901 cagcttatga ggaaactctt taccggactc tttttcatgt ccttaactgc ggcaggtttt
     3961 gcacaagacc tggccatggc tccaccgctg ggtacgtttt tgtcgcccgc aacaggatgc
     4021 tcactaacgg caactgaaac cgtaacgatc cgaatcttca atgcaggacc aaccattttg
     4081 gcgggaacca atattccggt ttcttattcc attaatgcag gtgctccggt aaatgaggtg
     4141 attactctcg gtgcaaattt tgtaaacaac acaacgctca actatacgtt cacggcggtt
     4201 gctgatttat cggttccagg ttcgtattcg attgatgcga ctgttgcact tccgggtgat
     4261 gttgttcctg ccaacaatac ctattccggt tttgtagtgg ttaacaatgc tgcatccaat
     4321 ggaggaacag caacgggtgg taccaatgtt tgtattactt ccaactcggg aaatattacg
     4381 ttggccggta atgttggtag tgtgttaaac tgggaatatt ccattgatgg tggtggtacc
     4441 tggattaaca tttccaatac aacaaccact caatcgtata acaatcttac tgttccaacg
     4501 caataccgtg ctaacgtaca aaacgcatct tgtccaaccg ctacttcaac tgttgcctca
     4561 atgacaattg atcctgcatc agttggcgga accgtaaccg gtggaacaac gccggcatgc
     4621 tctggtgcaa acagcggaac acttaccagt tcaggtagaa caggtaccat agtgcgttgg
     4681 gaattcacta tcaatggtgg tgcaacctgg acacccattg caaatacaac acctactcaa
     4741 aattatctaa atctggtaac aacaacggga taccgtgtag tcgttcaaag tggagcgtgt
     4801 gcaacagccg tttctgctac tcgtacaatt gttgttagtc ccccaacagt tgcaggtgca
     4861 gtaaattcaa atgcaacagt ttgttcagga acaaatagcg gaacattaac cttaaccggg
     4921 aatgtaggag cagtagtaga ttggcagttt tctaccaatg gtggagcaac atggacaaca
     4981 atagcaaata ctacaacaac acaaaattat ctcaacctta cgcaaacaac gatgtaccgt
     5041 gcaagagtaa gaagcaaccc ttgtgctact ttaatctcca cgccagctac aataacagtt
     5101 gttgcggcat caataggagg aacactttca tccaatgcaa cagtttgtat caattcaaat
     5161 agagatacac ttcgactatc aggtcaggtt ggaactattt tgaattggga gttttccaat
     5221 gataacggag ttacatggaa caactacgct tcaacaaacg atacgcttat ttatgataac
     5281 cttcaggcat cacgtaacta ccgggtaatc gtacaggccg gtggttgtgc tgcggttaat
     5341 tcttcagtcg ttgctattac agtagatacg ctaactgtag gtggaattgc ttccggtgca
     5401 gtaacggttt gcgcaaatgg taacgcagga aatgtgcagg tgaccgggaa taatggtgta
     5461 atcaaccatt gggaattttc ggatgatgcg ggtgtaacct ggaatccgat tgcaaataca
     5521 acaagtacac aaccgtatac caacctatct gcttcaagaa ggttccgtgt ggaagcgcaa
     5581 aatggagttt gtcctgtagg ttattcaaca gaagtagaaa tttcagttga tgcaattacg
     5641 gttgcaggat ccgtttctgc gagcacaacg gtttgctccg gcgctaacgc aggcacactt
     5701 aatctagttg gtaataccgg taacgtttta aattgggaac tttctaccga tggtggtctt
     5761 tcctggatta ccgtggccaa tactactact tcacagaact acgccaatat ttcgcaaata
     5821 acacaataca gagctaatgt tcagaatggt gtttgtccat ctgctatttc cggttttgca
     5881 gttcttacta tcgatgctca ggcagtagga ggaacacttt acggtggtgc tacagtttgt
     5941 gaaacggcta acagcggaac gctgacattg gttgggtata atgcttcaat tgttgaatgg
     6001 caagaatcta ttgatggtgg agttacattt actccgatcg ctaatacttc tcctttctat
     6061 aactttagta acctcaccac aacaactgaa taccgtgtta tcactgcttc aggtgtttgt
     6121 ccgaatgata tttcttcgtc tacagtggta attgttgatg cagcaaccgc gtcaggaatc
     6181 gtttctgcta acgctattgt ttgtgcgggt gcgaattctg ggacattgac tttaaatggt
     6241 cataccggtt cagtaaccaa ttgggaaatg tcgaacgata atggaaccac atggattaca
     6301 cttgccaaca atacaatttc tcaggattat ctgaatctga tgacaacaac ttctttcaga
     6361 gcgaaagttc agagtggtgt atgtccggca attggttcta ctcctgcaac gatcacagtt
     6421 aatgcacagt caaacggcgg agaagttaac tcttcggcaa cagtttgtgc aggacagaat
     6481 tttggtgttc tgaatctctt tggtcaggtt ggaaatatca ttgattggga attttcaacc
     6541 gatcaaggta caacatggac tcccattgga aataccagta caactcagaa ctacgctaac
     6601 cttgttgata caacctggta ccgtgtaatc gttcagagtg gaatttgtcc gatagattct
     6661 tctgattatg cattcatcaa cctttatcct tctccggctg caagtttcaa cggtgatacg
     6721 gtttgtcaga atcagccgtt ggcatttgta aatactacta ccaacccgat tggcttcgtt
     6781 acattgtatt catgggattt cggagatgga ggaacttcac tttctgccaa tccaagtcac
     6841 atttattctt ccttcggaac gttcactgcc agtttatttg caatgaacag ttttggttgt
     6901 acagataccg ccatgcgtac aattttggta aataatcttc cggtagcgga tatcttccct
     6961 agtggaccaa caagtttctg tgatggagga tctgttgatc tttctgcgcc attcaacatc
     7021 aactattcct atttgtggaa tacaggcgac aacaatttcc agattactgc agatactaca
     7081 ttcaattaca tagttactgt tacggacatc accaacggat gcgtgaatac ggattcggaa
     7141 atggtaactg catttcctct tccggttgca aatgccgggc tcgatacaac agttagtctt
     7201 gggtatggtg taatgcttaa cggatccggc ggagttactt atttgtggtc gccttcactt
     7261 ggactggata atcccagctt cccaacaccg atggctactc caacaacaac cacaacctat
     7321 attttaactg ttacaagtat tgacggatgt accgatagtg attcggtaac ggttgttgtc
     7381 aataatgatt tccttgtaac tgttcgcaac atcattacac cgaacgcgga tgggttcaat
     7441 gatacctgga tcgtagaaaa tattgaaagt tatccgaaca atacgatcac cgtatttaat
     7501 cgtaatggtc agaaagtttt tgaaacagac gcttacatga acgattgggg cggaacattt
     7561 ggcagtgatc ctctgcccga tggtacctac tactacgtga tcaaattcaa cgacagcgat
     7621 attgtcgtga aaggcgcagt aaacattatc cgttcatcaa gcaactaaaa ccacggcagt
     7681 catgaaaaag ttactctata cttcagtatt ggccgcaatg gttgcggtaa atgcaaaggc
     7741 gcagcaattg cccttgttta gtcagtatta ttataacaag ttcctctaca atcctgcttt
     7801 cacaggtaca gaagatatgg gacaggctta cctgattcac cgttcgcagt ggaaagatat
     7861 gccgggctct cccgttactt atgccttgac tgttgatggt ccggtaaaat ccaatcagat
     7921 cggattggga atgtcgttgt tcaacgatca aacggatatt ttcaatcgca caggtcttta
     7981 cacatcctat tcttaccgtt ggaaagtgaa cgaagaccat cacctcacat ttggtttgtc
     8041 gggtggagtt atcgataaca agattgattt tgcacgtgtt tctgcacgtg atgttaatga
     8101 tccattgctt tacaatgaaa accgtagaaa gattaccatg gatgccaatt ttggtgtcgg
     8161 ttattttttc aaagatcttc aggtaggttt ttctatcccg caactattgg gaaataaagt
     8221 aaaatacaat gaaggcgcaa caagtgtcta cacagtaaac aaacgtcatt tcgttggaag
     8281 tgtttcttat tcacttatga tcagcgaaac acagcaaatt cgtttcattc cgaacatcat
     8341 gacccgctat gcgaagggtt ctcctttcca atttgatttc aatgcgaatt tcgattggaa
     8401 agacatggtt cgtgcaggct tgtcgtaccg tcacggttat gccgttggta tgaatatcgg
     8461 agcgaaggtg aataagaatc tggttgccgg ttatacctac gagtatgtaa tttctccaat
     8521 cggtgcagta gccggtggag gacatgaaat tctattggga taccgctttt caggcggagg
     8581 tcgtggaggt gatgacgaac agatcaagcg aatgaatgag aatcttgacc gtgctcaaat
     8641 ggagaacgac agtctgatgc gtgagctcaa gaaaaaagac caggaacata caacggatat
     8701 ccaatacctg aaagaggaaa tggaaaagtt gaagaaacaa caagaggaag gaaataacgg
     8761 aacttccggg aaaaatcatc ctccaattga agagatcaat aaaaatatcc gttctgaaaa
     8821 agctgccgat tatcagaacg aagatggtac acctttaact cccggttatt atgtgatcat
     8881 gggaagcttt aaagtaaaag agaacgctca gaaatcgaag aaggattatt caaccaagcc
     8941 cggatacaat ccaactgtat tgttcaataa agtgcgtggc ttctattatg taaatgtgtt
     9001 ttattctact gatgaagaaa cagcattgca aattatggaa gtgttacgta cagaacaacc
     9061 tgatgcgtgg gtattcaaaa tggaataata gattgcagca acattcaaaa aagatcccgt
     9121 gaaatcggga tctttttatt ttatacgcat caggatttct ctgaattcac tcaggattac
     9181 tgcagttgcg ccccaaacaa tttctccttt gatgtcgaag taggggacgg tcatgttaag
     9241 tccggtggaa agcttgattt tagtttcctt gatcaactca tccctggtta attcatcaac
     9301 aggaaattca ataatgtagt ttacctcgcg aggatcagga ttgaatgctg gttgttcagg
     9361 aagccagctt aatacagggt ttaccagaaa attggatgga ggaatatata cttctgtcag
     9421 cgaaccaata atctgcgtat cgggaaccca caccccaatc tcttccttgg tttcacgaat
     9481 agatgtgtgg atcagactcg catcactgtc ctcttttctg cccccgggga aacttacttg
     9541 tgcactatgg gttccctcgt attcgtttcg ctttgttaat accaggtgcc attgattgtc
     9601 tttaagataa atgagagcca ttaccgcact taaacggggt tccttgcctg caccgtccag
     9661 ttctgccttt accgatcttc ggtagggcgc cattttcagg tgagcttcct cgccaggtag
     9721 ctgttccgtc agttggcgtt gaagacgatc gatgaagtcc attcacaaat gtactttcat
     9781 aatggttcat tccgtacttt tgacgatcat aaaacatttc catggaaggt ttcgatttcc
     9841 ttttttcagt tgatggtgta attgcatttc tcactcttgc agtgctggaa attgtactgg
     9901 gtattgataa cctggttttc atttcaatta ttgtagacaa agcacccaaa gagaaacaac
     9961 ggtttatccg actccttggt ttaggacttg cgcttggttt cagggtggtg atgctgatgg
    10021 gtatttcctt tattgtggga ttgaaagctc cgatattcag tatgggtaac catgaattct
    10081 cctggcggga tctgatcctt ttgggtggtg gtctattttt gattgcaaaa agtacactgg
    10141 agattcacca taaactgaaa agtgcaggaa tggttgaaca tcataaagag gaaaaaaaga
    10201 caagtgcaaa aaatgtagtc ctcaacatga ttacacaaat cgttctgatt gatattattt
    10261 tttccatcga ctccatcctc accgcagttg gattaacaga aaacgtaaca ctgatgattt
    10321 ctgctgttgt gatttcgatg atatttatga tggtattttc cggtcctgtt ggtacattca
    10381 tcaaccagaa tccaaccatt gtgatgttgg cattatcctt ccttctgatg attggaatgc
    10441 tacttgttgc agaagcattc ggtcaggagt tcgaccgcgg atacgtctat tttgcaatgg
    10501 gcttttcgct aacagtagaa ttactgaatc tggtactgct caaacgcctc taccgcagca
    10561 agccttccga cgagaattga ttgtttaaat tggatttaat agctattttt gcaccccatt
    10621 cagcccggat ggcgaaattg gtaaacgttg cggtctcaga agccgttgga gtaacttcct
    10681 tgtgagttcg agtctcactt cgggcaccat tgaaatttaa gcctcacgan nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt agtgaggctt tttaaattaa ggtagaagtg
    10861 aggcgagaag ttcacccgcc gaaataatat gcaggcggga gtctcacttc gggcaccatt
    10921 gaaatttaag cctcacgata gtgaggcttt ttaaattaag gtagaagtga ggcgagaagt
    10981 tcacccgccg aaacaatgtg caggcgggag tctcacttcg ggcaccattg aaattcaagc
    11041 ctcactatgg tgaggctttt ttatttctaa gtgtttctaa gtgtcctaag cgtctcagtg
    11101 gtgaaaaaaa accggagcaa atctctaagt gtatcttagt gcccttcgtg tcttagtggt
    11161 gaaaaaaccg ggactaaaaa cttatccacc ctcattctcc ctgatcaaca ccttaatccg
    11221 cttatcatcc ccaaccagcc aaagttcatc gccttcttca aaaacgacat cggatgaagg
    11281 attcaggata cgcacttcat ttcgaacaat ccctacaata attcctgacg ttcgctctct
    11341 taaaccggaa gcacgaatgg atttaccgac cagggaggaa tttggggtca gtgtaaagtg
    11401 tcgtaattga atgttttttt caaggtttgc ccatggacct gtggcaattt caaattcatt
    11461 caaaaagttt ctgaattttt cgagctgctc gtcggtacca ataatatgca attgatctcc
    11521 cgggaaaatt ttctcgtgcc gggttggaat atacaggttt acatttcccc gttcaatcat
    11581 ggcaatatta attccaaatt cttcgcggag tcggatttct tcaagcgatt ttccgaggaa
    11641 ggggaggttg ggaccaattt gcacaactgc catatgggta tcccatggag cgaggttttg
    11701 atccggttct ttactgcggt tttcctgtcc cgaccaattg ctgatgaatc gctgttcgat
    11761 gcgtgaataa aaatcgcgga tacgaaaacg aaggaggaat aagacaaaaa tactaaccac
    11821 aataaccatt agcgcaacca atggagtaaa taccaggtta aaaaagaagc ccatgaaaaa
    11881 gagcatcaat agcaagcgga acaattgcat tacaacaagc aatccgcgtt gcgatggctt
    11941 aagccatagt tcagaatagg ctgttttatc ggtccgtttg aacgcaagcg cccataaaaa
    12001 aggtgaagct gcaagcaaaa caatactgca aaacaaaatt gcagagaaag aatcgtaatg
    12061 gaatttggga aacaaaaagc gaacagaaag gagaaaaaac gcgaggatga taactgaata
    12121 gatcacgatg ttctttacgt aagcgcgcag gtaccgtttc caggcgcttg tttcaggtac
    12181 gcgttgaagg ctaagggaat atttttccag tgcagatcgc caggaagacg gcattccccg
    12241 atccacaata ccatagaagg gtttgctgaa acgaatcatg tagggtgtag tgaatgtagt
    12301 aaatgcagaa acagctactg ctaccggata aagaaaatca ctcgtcacgt ttaatgtcaa
    12361 tcccaatgaa gcgatgataa acgaaaattc accaatctgc gaaagactca ttcccgatcg
    12421 catggatact tttagtgttt gtccgctcac caatgcaccg gtagtaataa aaagtggttt
    12481 accgataatc aaaatcagac ttgctccaac gatcacccaa agatgttctg ctatcatcgc
    12541 aggatcgatc agcattccta ctgatacaaa aaagatcgca ccgaataaat ctttcaatgg
    12601 ctgaataagg tgttcgatac ggtgacctga attcgtttct gcgagcaatg atcccatgat
    12661 gaatgctccc aatgcggaag agaatcctgc ttttgttgcg agtattacca tcaggaaaca
    12721 caatgccaga gaaacgatga gtaaggtttc gtccgacaaa aaccgtttaa gccgacgaaa
    12781 taatccgggt agaaaaaaca ctccggtaat tacccaaagt atgagaaaga atatcagttt
    12841 aaaaatggag aacatcattt ctgatccttc aaagtttcta ctgactgaaa tcgtacttag
    12901 tattaccatc aataccactg ccaccaaatc ctcaacaacc agaactccca ttaccacact
    12961 tgcaaaaagc tggttttttt ctcccgattc atcaaatgcg cgaatgataa ttgttgtaga
    13021 tgctatactt agaatacctc ccatgaataa ggcatccatt ggtttccaac ccaaaaaagt
    13081 tccaaataaa taaccgaaga aaaaagtgaa tgcgattccg aacagtgccg tgatgattgc
    13141 tgttttaccg actttaaaaa gtttccgaaa actgaattcg agtcctaaac cgaaaagcaa
    13201 aaacaataca cctatctctg cccaggtctg aattcctttt agttcagata ccgttggaaa
    13261 caaatcgaaa tgaggaccaa caagaatacc tgccatgagg tagcccaata ccagtggttg
    13321 tttcaaaaag cgaaacagca aagtggtaat ggccgctaca ccaagtatca gggttaaatc
    13381 ctgtatgata tgcggtacat gattcataaa tcagatttcg gtattccgaa atgaggcttt
    13441 acttttttca gatgaatgat gctgaaagaa tatttaaatt gctatcgagc tcgtatttct
    13501 tcggagcacc ggttggaatt tctacctgga gaatttgctc aggactcaac ttctcaagat
    13561 acatgatcaa cgctcttaag ctatttccat gcgcagcaac aatgatgttt ttgccggatt
    13621 ttaatttagg aacgatctcc ttttcaaagt agggaagtac ccgatcaagt gtatctttta
    13681 aactttctcc tttcggcggt gcaatatcaa atgaacgacg ccagatgtga acttgttcgt
    13741 cgccatattt cgctgctgtt tcggctttgt tggcgccttg taaatcgccg tagtgacgct
    13801 cattcagcgc ttcgtttttt gttacaggga tgttctcttg tccgatttct ttcagaataa
    13861 tcgaaagtgt acgttgcgca cgcagcagat tggaggtgta cgcaagatca aactttagcg
    13921 atttcagttt ctctcctgca tttattgctt ctttttctcc ttgctcggtg atgtccacat
    13981 ccacccatcc ggtgaagcgg ttttcgaggt tccaggctga ttgtccgtga cggacgagga
    14041 ctagtgttga catttttttt tttagccggg gactaaagtc cccggttacg gttattggtt
    14101 gttttctttt aagatccggg gactgaagcc cccatctact attccgggga cttaagcccc
    14161 cggttacggt cttcgcttag ctatcccggg gactgaagcc cccggttacg gtcttcgctt
    14221 cacgaatttt ttggtgaagt ggtagaggag acgcactcca actccggatc ctcctttgtg
    14281 gtggtagtcc tttgcatctt ttaagaacgc aggtcctgcg atgtcaaggt ggatccagtt
    14341 gtaatccgta aagcgtgcaa ggaacttacc ggcagtgatt gctccgcctg ttggacctcc
    14401 aacatttcgg atatctgcaa tatcgctctt gatcagatca tcgtattctc tccagaacgg
    14461 catttccgca agacgttcgt aggtttcatc gccggtttct atgagttctt ttttgtactt
    14521 gctttcattg cccattacgg cgcttccata ctgaccggta atcattgctg cagcacctgt
    14581 gagcgtggca agatcgatca ccaattcggg tttgaatttt ttggcatacg ctaatgcatc
    14641 cgcaagaatc agacgacctt ctgcatcggt attctgcact tccaccgtag taccatcgtg
    14701 catggtaatc acatcatcca caaccagcgc atgactgtta atgcggttgt ctgttgcagg
    14761 aatgagtccg attacataaa ccggaagttt atttttagca atagcagaaa tcgctccaac
    14821 aacagcagca gctccggcca tatcgcattt catggtttgc attacaccac ccactttaag
    14881 cgaataacct ccggtatcgt aaacaactcc tttgccaaca aaaatgagtg gcttcgtgtt
    14941 ttttgctcc
//
LOCUS       DAOMYG010000016        14918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1365_length_14918_cov_9.803808, whole genome shotgun sequence.
ACCESSION   DAOMYG010000016 DAOMYG010000000
VERSION     DAOMYG010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 14918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14918
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1365_length_14918_cov_9.803808"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2590
                     /locus_tag="PK610_01215"
     CDS             <1..2590
                     /locus_tag="PK610_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HRJ34570.1"
                     /translation="NKAILLSQGEFARFLKESQNEQLELLEQLSGTAHFRHIGSAIYE
                     KFSNGKKSIETQEAVLSGIELLSEEQIQEKKTSISKSILQIEKLQPETEHYRLLLQVK
                     QNIKKENEILNRKKKQFEDLLEKEKAVEKERKRLSDHELASPYRQEIFRVQAFENEHR
                     LLKDQFEQLHKRQEELQLEEQDLLSDVRFLLTAVTTLSDAQTSLPLVLEKFEEISDAI
                     KKTEIEIRALNDQIKPQFESLSFKETIKEKLKAGAEITEVLKTHLEELKQRKLNLDKR
                     FNLSEKKPEQRKEQITQWMVAVEACLLREQSIDAESHSVEKLKTELANIINNVESKTP
                     ELARIIAAEDLLLRSIKNLEEKEQIERDKAQLVDFRHLLIQGEPCPLCGSETHGEHQH
                     SPNPDSVKKQLEEEKNKAGLLSQNKRALESEINRLRDQHLFLKGELEKKETFISQSRQ
                     EQIRVIDKIKTESGLSESSVNDLLVLLKKENETISEYAELLKDENGSIALLQLAEKKK
                     ILTTQEKELNLSLQDLTHGLPLKEKTSTWIKQIHALEKERPEIQKQRVRIEKQILELN
                     TTLSDVTTDLLKRLNDSGIATIAECKLYLLPEKEEQELRSQFQLLEREKTALQSGISE
                     STERIKSLASSDNSTIDEGKAQELFIQAEKQLNENKEIRTRFQTELNADQVNRQRFLK
                     GQSEIEKTRKDLRKWEILNHLLGDATGKRISAIVQEITLTRLLKHTNIRLTHLSPRYR
                     LVKSELRGGELRVEDAFLGNTLRDVRTLSGGETFLLSLALALGLSDLASRNVRLESLF
                     IDEGFGSLDQETLETAISTLEKLQTEDNKIIGIISHVEALKERISAQVQVSRSGKGTS
                     RISIVG"
     gene            2730..3800
                     /locus_tag="PK610_01220"
     CDS             2730..3800
                     /locus_tag="PK610_01220"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HRJ34571.1"
                     /translation="MATVQFSRPGSDFFQSLRNSVHAYFESNKISQTGDYRLYIKTLF
                     FCLSGLAIYISLLTVAMPWWVALILAGTLGLFQAFIGFNVMHDACHGSYSSKHKINNF
                     LALSMNVLGSDAFMWKQKHNLVHHTYTNIDGIDDDIQKAPLIRMAPSQPKFWAHRFQF
                     IYVVPLYAVSSFFWILLKDFQEYFSVKKYAVKVDKMSTMEHVTFWVSKSLYMFFYIVL
                     PILVWGWLPWFIGFLTMHITLGLTLSLVFQLAHVVENVEFHDARDKEHTLVETEWAIH
                     QLKTTSDFAVDNKFVSWFLGGLNFQVEHHLFPRISHVHYPAIQKIVKETCEKYGVEYH
                     SYKNTRLAMASHFRHLYNLGRK"
     gene            3802..4524
                     /locus_tag="PK610_01225"
     CDS             3802..4524
                     /locus_tag="PK610_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34572.1"
                     /translation="MYSIELLHTSGGYRFNYKSGDDEKIITWAGTDTAMNIPDKVLAL
                     RMKESLEKYPMYFDDNDNLIIPSPLYFLMSVAHTGSSSLSSQIENHALFWSQDSFFHR
                     AEDERILAAQQGVVRPVEQYLLEKGFRTTILPNADYSSWEDLSEEDLLPEEDWKKLQS
                     LFDSLSEKEKIAVLYLAFNSPHVVAAIAWVNNAFPNEESFAHYSLVMEGIISPHTMHP
                     ADFEREVRLREDMFAEVRFFMG"
     gene            4608..5432
                     /locus_tag="PK610_01230"
     CDS             4608..5432
                     /locus_tag="PK610_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRJ34573.1"
                     /translation="MAQKTYIVPHDFSSVADTALNHASAVAKITGAEIHLLHVVSKPA
                     QVDDTKGKLESLAAETAKKNGVVCKAHIRIGNIFDDIGDFGKEINAALIFMGTHGAKG
                     WQKIVGSDAMKVITNSEIPFIVVQEKNIKESGYDDIVVPMDLERDTKQKLTIVADMAK
                     YFKSKVHIIVPKEDDEYLVHQMNSNIAFARKFLNDRGIDFTTTIADGSLEKECVRLAS
                     SIDADLIAIMNHQGNRLTGGLLANSAEQHIITNEAQIPVVIVNPQDTGVSGSILFS"
     gene            complement(5515..9402)
                     /locus_tag="PK610_01235"
     CDS             complement(5515..9402)
                     /locus_tag="PK610_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRJ34574.1"
                     /translation="MFRLFSLLLLSVSFLSLRAQNKAVRFDNMDINDGLSQSLVNDLI
                     QDRQGFIWIATQDGLNKYDGARIVIYKKDPTDQQSLSNNFIHALYEDKEGFIWLATNG
                     GGVNRFDPRTNTFKRYDLGPQNRTVTDITEDSEGNIWFSTMGNGVNKLNKEKGRVITY
                     VFKGVEARNVQCVYSDSKGRVFAGSSGQGLLMYNKTHERFDRYVNPLIDDVIWCITED
                     KKGTIWVGTNSGLVSMTPSGDEFVCKRFINDYSSPHSLSNNSVRCLHITRDQLMIVGT
                     TGSGIDIMDLDRPGYFINHKHEDSDDGSLADNLVQSVYEDRSGSIWIGTNEGLSRFDP
                     LKQVFGQINHSPDDINTINDRTVWSIYEDQQGILWVGTRKGLNRVDRRNNLVFRYDRK
                     ANNPNAIGNNSVLSLYVDAHLRVWVGAVDGLYRLELSDNLRLAVFVPVQFRPPGNGFA
                     DNRVYSITEDTRNKCLWVGTKEGLSCINLSSGSYKFYTNNPNDETSLPSNSVRFVYID
                     NEGKTWVSTDGEGLSLVEKNETGQLRFRNLKKQLSPKGGEEDLLLTSIWEDEKGMFWL
                     GSYGSGLLHFDPKQEKLIRTYTEKDGLSNNAVYAVLGDEKGHLWMSTNYGLSSLDPIT
                     GKMRRYLEKDGLQSNEFNTGAFHKSKTGELFFGGIQGLNNFYPSDLKDNVVPPDVVIT
                     EVLLFNKPLSSYENLHIKKSASYLEELDLSYRENSLTISFAALHYSNPSSNRFRFIME
                     GLDDEYIEAGTTSLANYNKIPYGSYTFKVYACNSDGVWSEKPAELKITITPPFWATWW
                     FRSLLLVFLVGGVFAFNRARLINIKKQRLQLAREVRDRTWEVMEQKEKIEQQKKLLEE
                     EKNKVEKLLLNILPEETAEELKSRGKASARSYRQVTVLFTDFVGFTKIAERVQPSDLV
                     ARLDAYFIRFDEIIQKYNLEKIKTIGDSYMAAGGVPIRSKSNPIDTVLAALEIQRYMV
                     ELQESAGTTGEEPWELRIGINTGEVIAGVIGTKRFAYDIWGNTVNTANRMQMTCDPMM
                     VNISGTTFELVEPYFECTYRGKVAAKNKGEIDMYYVHRIKPELSVDGLGLEPNEKFWK
                     YVDLYLYSSINYGKAERHIMKVLKDGLPSHLYYHGIHHTYDVVQAAERLALMEGITDE
                     QMFILKSAATYHDAGFVEQYAKNEPIGIRMAQEILPKYGYTEEQVEEVAKLIRATIIP
                     HNPQSHLEEIICDADLDYLGRDDFHEIADTLRRELREQGVINSDRGWDEMQVKFLNMH
                     KYFTKSAIRLRQEKKEKHIQDIIQKLESFSYKD"
     gene            complement(9480..11309)
                     /locus_tag="PK610_01240"
     CDS             complement(9480..11309)
                     /locus_tag="PK610_01240"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRJ34575.1"
                     /translation="MKFRFNIGRRIGLGFGVVIFFITVIFSVTVQNVISGLSIFEESK
                     KLNEQITRIETPSVNLVIELSNLIQESKALITKWATEQSRDDNPDKLRLVALMNKELP
                     RVKGQIKEFTTYWPQEEVTIIDSVFNNIEQLTGLYTQIQELLVTFEDYSDAERALMAR
                     FLVETDGDVDREVKMIMMSLNRLKRMQEMRLKNTRDAQVVSSNATMGSYKFLIYLLLI
                     SGVGLVVGSILIATFTSRSIVRPVARLKSSLQMLGKGIIPEEQISPRDDEIGEMTIAM
                     NNLIDGFRRTTDFSRQVGIGNFNYQYRPLSDQDVLGHALLKMRDDLAENERILEQKVI
                     ERTEEVVRQKEEIEKQRQKLEELYKDVTDSIRYAKRLQESILPPDMMVRQLLPDSFVL
                     FKPKDIVSGDFYWFYKRENKVLFAAVDCTGHGVPGAFMSLVGSNALNQAVKEHGHTEP
                     ARILDDLNKLSSEALNKNKENNSVRDGMDLAFCSLHTDTLELEYAGANNPLYIVRDGE
                     LLQVKADKIAIGAFEPGARKYTNHKMQLKKGDAIYVFSDGFADQFGGMQGKKFMYRQF
                     RDLLIGMKDKTMDVQRKLLHDSIEKWRGNYEQVDDILVIGVRV"
     gene            complement(11331..12203)
                     /locus_tag="PK610_01245"
     CDS             complement(11331..12203)
                     /locus_tag="PK610_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ34576.1"
                     /translation="MLISGSTAFAQGPVAERALQAYQGNEWDKAARIIDSASVASGEA
                     ADPFTWHLRGFIYKDIYKIAQKENRNSTARAIAIESFYKSNELDKAGDFTQRNNGSIR
                     SLVISYFNDAVRLMDTTNYMESEKFYLEYKRQMKRFAPATDFMKSDVEYYNAFATILV
                     KKFNRMEKGADVFFNKAIEVYQKVIELDSNNCLAHYQIGILYYNKGVEILLNLDPTTP
                     LEEIIKSQEICVELFQKSKPHMYKAWELQNCKDINPLEIAEGLSGIHYQLNEPDKFKY
                     WEDLKKKLQEGEDK"
     gene            complement(12235..12759)
                     /locus_tag="PK610_01250"
     CDS             complement(12235..12759)
                     /locus_tag="PK610_01250"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HRJ34577.1"
                     /translation="MKRWFLIALLVFPTLAMDMLSGDPKDTHKTVKALFVYQFATLVD
                     WPKEFKKGDFIIGVYGESPLYDELTNKYSNRSVGSQAIKIRSFQSYNSITPCHILVVA
                     PEVSDKVADLAKKFKSSSTLIVSEKEGKLKEGAVISFVIKDNKQSYELSKSNASKSKL
                     IIGSKLENLAARVE"
     gene            complement(12862..13638)
                     /locus_tag="PK610_01255"
     CDS             complement(12862..13638)
                     /locus_tag="PK610_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34578.1"
                     /translation="MYLFRGLFVFFLALSLSGQLNAQAGKRSKVSDGKETYNSSRSKK
                     KQQKTRSTFTAKPNKKRSNRDKDAFVARSRKKDNAKPADHFVASSRPKKKSKESDSFS
                     ARRRKANGGSGDDSFKSRGRKPSGGSGDDSFKSQGRKPRVVTEDSFVARGKKPSSGGG
                     KDAFSTKTSTRKKSVEKSDPFSGKGKRKKAKAGEGYGKKKLKLFRSKEERKRVKKERK
                     ERHDPEFRQSQRKIERKNRRERSKNKGNDALPKPMLKQNY"
     gene            complement(13641..14483)
                     /locus_tag="PK610_01260"
     CDS             complement(13641..14483)
                     /locus_tag="PK610_01260"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRJ34579.1"
                     /translation="MKQHVYQLSHTKKPRSVNFQSGLDEASAYIRGSLDSIGQGWRKQ
                     EYRVDSLRVHNIIAFYGDTTLPRLVIGAHYDVCMNLPGADDNASGVAGLLELSRRLNI
                     EKPRLKHCIEMVFYTLEEPPHFRTVNMGSYVHAKDLADRKVNVEAMVCLEMIGFYSDE
                     KGSQQYPANIMKLWYPNRGNFIAVVGKKGMKDLGSVFKKGINKYSNLPCEMLIAPSRV
                     EGVDFSDHLNYWLFGFKAIMLTDSAFMRNKNYHKRTDTWDTLDYEKMAELVEGLFRTL
                     KTYY"
     gene            complement(14575..>14918)
                     /locus_tag="PK610_01265"
     CDS             complement(14575..>14918)
                     /locus_tag="PK610_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013621300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HRJ34580.1"
                     /translation="VNERGGHAVIPALSQPPKTFKDLHFVFEKLLQHEILVSAEINKV
                     VDVCLREKDYSTHNFMQWYVSEQIEEEALARTIMDKLRMIGNDKGGMYLFDRDIQTLT
                     ANTAGGPANKA"
BASE COUNT         4285 a         3442 c         2916 g         4275 t
ORIGIN      
        1 caataaagcc attcttctat cgcagggaga atttgcccgc tttttaaagg aaagtcagaa
       61 cgaacaactg gaacttctgg agcagctttc aggcacagca catttccggc atatcggcag
      121 tgctatctat gaaaaatttt caaacggaaa aaaaagcatt gaaacgcaag aggcggtatt
      181 aagcggaatt gagcttttga gcgaagaaca aatacaggag aaaaaaacaa gcatcagtaa
      241 aagcatactt caaattgaaa aactccaacc cgaaacagaa cactaccgtc tgctgctgca
      301 ggtaaaacaa aacatcaaaa aggaaaatga aatactgaac cggaaaaaaa agcaatttga
      361 agatctgctt gaaaaagaaa aagccgtaga aaaagaacga aaaaggctat ccgatcacga
      421 attagcatct ccttaccgtc aggagatatt ccgggttcag gcttttgaaa atgaacaccg
      481 tcttttaaaa gatcaattcg aacaactcca caaacgacag gaagaacttc aattagaaga
      541 acaagattta cttagtgatg tccgattcct tttaaccgcc gttaccactt tgtctgatgc
      601 tcaaacatcg ctacccttgg tgttggaaaa attcgaagag atcagcgacg ccatcaagaa
      661 aacagaaatc gaaattcgcg cactcaatga tcagataaaa cctcagtttg aatcgctttc
      721 attcaaagaa accataaagg aaaaactgaa agcgggcgca gaaattactg aagttctgaa
      781 aacccatctg gaagagttaa agcagcgaaa actaaatctt gacaagcgtt ttaacctcag
      841 cgaaaaaaag ccggagcaaa ggaaagagca aatcacgcaa tggatggttg cagttgaagc
      901 ttgtttgctt agagaacaat ccatcgatgc agaaagccat tctgttgaaa agctgaaaac
      961 cgaactggca aacatcatca acaatgtaga atccaaaaca cccgaactcg cccgaataat
     1021 tgcagctgag gatttgcttt tacggtccat taaaaacctc gaggaaaaag aacaaataga
     1081 acgcgataaa gcccaacttg ttgattttcg tcatttactg atccaaggag aaccttgtcc
     1141 gctatgcggt tcggaaacac atggcgagca tcagcattct cccaatcccg actccgttaa
     1201 aaaacaactg gaggaagaaa aaaacaaagc cggactgcta agccaaaaca aacgtgctct
     1261 tgagtccgaa attaaccgac tgcgcgatca gcatctgttt cttaaaggag agcttgaaaa
     1321 aaaagaaaca ttcatttctc aatcacgaca agaacaaatt cgtgtaatcg acaaaataaa
     1381 aacagaaagt ggtttgtccg aaagcagtgt aaacgatttg cttgtcctgc taaaaaaaga
     1441 aaatgaaacc atttcggaat atgcagaatt gctaaaagat gaaaatggat ctatcgctct
     1501 cctgcaactt gccgaaaaga aaaaaatact tacaacacag gaaaaagaac tcaacctttc
     1561 cttgcaagac cttacacatg gactcccact aaaagagaaa acaagtacat ggataaaaca
     1621 aattcatgca cttgaaaaag agaggccgga aatacaaaag cagcgggtaa gaatagaaaa
     1681 acaaattctt gagttgaata caacgctatc agatgtaacg acggatctgt tgaaacgatt
     1741 aaacgattcg ggtattgcaa ctattgccga atgcaaattg tatttgctgc ctgaaaaaga
     1801 agagcaagag ctacgatcac aatttcaatt gctggagcgc gaaaaaacag ccttgcaatc
     1861 aggtatttct gaaagtactg aacgaattaa atcacttgct tcgagcgata attccacgat
     1921 cgatgaaggc aaggcacagg agctattcat tcaggctgaa aagcagctca atgaaaataa
     1981 ggaaattcgt acacgctttc aaacagaact caatgcggat caggtcaacc gtcaacgctt
     2041 tttaaagggt cagtcagaaa ttgaaaaaac ccgaaaggat cttcgtaaat gggaaattct
     2101 caatcatctg cttggagatg caaccggaaa aaggatcagc gctattgtgc aggaaattac
     2161 gcttacgcgc ttgcttaagc ataccaatat ccgtcttaca catctttctc cacgctaccg
     2221 cctggtaaaa tctgaactcc gcggtggcga attgcgggta gaagatgctt ttcttggcaa
     2281 tacccttcgg gatgtaagaa ccctctccgg aggcgaaact tttttattga gtctcgcact
     2341 ggccttgggc ttatccgacc tggcttcgcg taatgtccga ctcgagtcgc tgtttatcga
     2401 cgaaggattt ggttcgctgg atcaggaaac gctggaaaca gccatcagca cactcgaaaa
     2461 acttcagacc gaagacaata aaattatcgg aatcatttca cacgtagaag ccttgaaaga
     2521 acgcatttct gctcaggttc aggttagccg ttcgggtaag ggtacgagta gaatttcaat
     2581 tgtagggtag gatttgattt gtttaagctt ttgctaaaaa ctcctggttt tcagtgccta
     2641 aacattgcat gatttgttaa tgaaaatcag aacagatttt ctttattcaa aagggtgtat
     2701 atttgcgccc gtcaccatta catcacgctt tggcaaccgt tcaattctcc cgccccggaa
     2761 gtgatttttt ccaatccctc cgtaattccg ttcacgctta tttcgaaagc aacaagatca
     2821 gtcagaccgg cgattaccgt ctttacatca aaacgctttt cttttgtctg agtggtttgg
     2881 ctatttacat cagtttgctg actgtagcaa tgccctggtg ggtggcgctg attcttgcag
     2941 gaacattggg cttatttcag gctttcattg gttttaatgt catgcacgac gcctgccatg
     3001 gcagttacag ctccaagcat aagatcaaca attttctggc tctcagcatg aatgtattgg
     3061 gcagcgatgc gtttatgtgg aaacaaaaac acaacctggt tcaccacacc tataccaata
     3121 tcgacggaat cgacgatgat attcagaaag ctccccttat ccgtatggct ccgagtcaac
     3181 cgaaattttg ggcacaccgg tttcagttca tttacgttgt tcctctttat gctgttagta
     3241 gctttttctg gattcttctc aaagattttc aggagtattt ctctgtaaaa aaatatgccg
     3301 taaaggttga caaaatgtcg accatggagc atgtcacatt ctgggtttcc aaaagtcttt
     3361 acatgttctt ttacatcgtt cttcctattc tggtttgggg atggttgccc tggttcattg
     3421 ggttcttaac gatgcatatc acgcttggat taacgctttc cctggttttt cagttggcac
     3481 atgttgtaga gaatgtggaa ttccacgatg cacgcgataa agaacacacc ctcgttgaaa
     3541 ccgaatgggc tattcaccag ttgaaaacca cttctgattt cgcggtcgac aataaatttg
     3601 taagctggtt tctcggcgga ttgaactttc aggtagaaca ccatttgttc ccccgaatca
     3661 gtcatgttca ttatcctgct attcagaaaa tcgtaaaaga gacctgcgaa aaatacggcg
     3721 tggaatatca ttcttacaaa aacactcgtc tggcaatggc atcacacttt cgtcaccttt
     3781 acaatctggg gcggaagtaa catgtacagc attgaacttt tacatacctc tggtggctat
     3841 cgctttaatt acaaaagcgg tgatgatgaa aaaatcataa cctgggccgg aacagatact
     3901 gcaatgaata ttcctgataa agtattggca ttgcgcatga aggaaagcct cgaaaaatac
     3961 cctatgtatt tcgatgataa cgataacctt atcatccctt ctccgcttta ctttttaatg
     4021 tcggttgctc ataccggatc atcgtcactc agcagtcaga tcgaaaacca cgcgctcttc
     4081 tggagtcagg attctttctt tcaccgagcg gaagacgaac gtattcttgc ggcacagcaa
     4141 ggtgttgttc gtcccgtaga acaatacctg ctcgaaaaag gattccgcac aactattttg
     4201 cccaacgccg attacagcag ttgggaagat ttaagcgaag aagacctgct ccccgaagag
     4261 gattggaaga aattgcaatc cctgttcgat tcgctaagtg aaaaagaaaa aatagccgtc
     4321 ttgtacctag cattcaattc gccgcatgtt gttgctgcga tagcatgggt caacaacgct
     4381 tttcctaatg aagaatcgtt tgctcactac tcacttgtaa tggaaggcat catcagtccc
     4441 cacaccatgc atccggccga ttttgaacgt gaagtgcggt taagagagga catgtttgcc
     4501 gaggtgcgct tctttatggg ctgataacaa gtcgcttaca aaagcccttg atagcaagga
     4561 gattggaata tttgattaaa tttgcttccc tgtcaaaacc aaattttatg gctcaaaaga
     4621 cctacatcgt tccacatgac ttttcttcgg tagcagatac agcactaaac catgcttctg
     4681 ctgttgcaaa aattaccggc gcagaaatcc atttgcttca cgttgtgagc aagcctgccc
     4741 aggtagacga taccaaagga aaactcgaat cacttgctgc agaaacagca aagaaaaacg
     4801 gggttgtttg caaagcacac atccgcatcg gaaatatttt tgatgacatc ggagatttcg
     4861 gaaaagaaat caatgccgca cttattttca tgggtacgca cggtgcaaaa ggatggcaga
     4921 aaatcgttgg atcagatgcc atgaaagtta ttaccaacag cgaaattcct ttcatcgttg
     4981 ttcaggaaaa aaacattaag gaatccggtt acgacgatat cgttgttcca atggacctcg
     5041 agcgcgatac caagcaaaaa ctgaccattg ttgcagatat ggcgaagtat ttcaaatcga
     5101 aggttcacat cattgttccg aaagaagacg acgaatacct cgtacaccag atgaattcaa
     5161 acattgcctt tgcacgtaaa ttccttaacg accgcggtat cgattttacc accaccattg
     5221 ctgatggctc actcgaaaag gaatgtgttc gtcttgcaag cagtattgat gccgacctta
     5281 tcgctatcat gaaccatcag gggaatcgcc ttacaggagg tttgcttgcc aactctgcag
     5341 aacagcacat cattaccaat gaagctcaaa ttccggttgt aattgttaat ccgcaagaca
     5401 ccggcgtgag tggatctatt ctctttagtt aattcgtctt tcatgaatat aaaaaccccg
     5461 catttagcgg ggttttttgt ttggaaatga tttcgaaatc ggaatacaaa accgctaatc
     5521 cttataacta aaactttcaa gtttctgaat aatatcctga atatgtttct cttttttctc
     5581 ctggcgcagc ctgatggctg atttggtaaa atacttatgc atgttcagga attttacctg
     5641 catttcatcc catccacggt cgctgttgat tactccctgt tcgcgcaact cccttctgag
     5701 cgtatctgca atttcatgaa aatcgtcgcg acctaaatag tcaagatctg catcacaaat
     5761 aatttcctcc agatgcgatt gcgggttgtg agggataatg gttgcgcgaa taagttttgc
     5821 cacttcttcc acctgttctt ccgtatagcc gtacttgggt agaatctcct gcgccattcg
     5881 gattccgata ggctcgttct tggcatattg ctccacaaat ccggcatcgt gataggttgc
     5941 agcactcttc aaaatgaaca tctgttcatc ggtaatacct tccatcagcg cgagacgttc
     6001 tgctgcctgc acaacatcat aggtatggtg aattccatga taatacaaat gcgatggcag
     6061 gccgtccttc aataccttca taatatgtcg ctctgccttt ccgtagttaa tggagctgta
     6121 aagataaaga tcgacgtact tccagaactt ttcattaggc tccagtccta atccgtcaac
     6181 cgataattcg ggtttaatac gatgtacgta gtacatatcg atctctccct tgttcttggc
     6241 tgctacttta ccgcggtatg tgcactcaaa ataaggctca accaattcga atgtcgttcc
     6301 cgaaatattc accatcatcg gatcacaggt catctgcatc cggttagcgg tattcaccgt
     6361 attcccccag atatcgtacg caaatcgctt ggtaccgata accccggcaa taacctctcc
     6421 agtattgatt ccgatacgca actcccaggg ctcttctccg gtagtaccgg cgctctcctg
     6481 aagttctacc atgtaccgct gaatttcgag cgctgcaagc acggtatcga tcggattact
     6541 cttactgcga atcggaacac ctccggcagc catataggag tctccgatgg tttttatttt
     6601 ttcaagattg tatttctgaa tgatttcatc gaagcggata aaataagcat ccagtcgcgc
     6661 aacgagatca gaaggctgaa cacgctctgc gatcttggta aaaccaacga aatcggtaaa
     6721 cagtacggta acctgacgat aactccgcgc actcgccttt ccgcgcgatt ttaattcttc
     6781 cgctgtttcc tccggtagaa tatttaataa caacttttct actttgtttt tttcttcttc
     6841 cagtaatttc ttttgctgtt cgatcttctc cttctgctcc attacctccc acgtacgatc
     6901 gcgtacttcg cgggccagtt gcaatcgctg ctttttgata ttgatcaaac gcgcacggtt
     6961 aaatgcaaaa acacctccca ccaaaaacac caacaagagg gaacggaacc accatgtggc
     7021 ccaaaatggc ggtgtaatgg tgatctttaa ttccgccggt ttctccgacc atacaccatc
     7081 gctgttgcag gcataaacct tgaaggtata agaaccatac ggtatcttat tgtaattggc
     7141 aaggcttgtt gttccggctt cgatgtattc atcgtccagg ccttccataa taaaccgaaa
     7201 acgattgctg gaggggttgg aataatgcag tgctgcaaaa cttattgtga gactgttttc
     7261 tctgtaggat aaatcaagtt cttcaagata agatgcagac tttttaatat gcagattttc
     7321 gtaactgctc aatggtttat taaacaacaa aacctccgtt atcaccacgt cgggaggaac
     7381 aacattgtcc ttaagatctg aagggtaaaa attattcagg ccctgtattc cgccaaaaaa
     7441 caattctccc gttttgcttt tgtgaaatgc tccggtatta aactcattac tctgtaatcc
     7501 gtctttctcg agatagcggc gcatttttcc ggttattgga tccagagaag aaagaccata
     7561 attggtgctc atccagaggt gacccttttc atcgcccaga acagcatata ccgcgttgtt
     7621 cgacaacccg tccttttctg tatacgtgcg aatcaatttt tcctgcttcg gatcaaaatg
     7681 aagtaatcca ctgccataac tacccaacca aaacattccc ttttcgtctt cccaaatact
     7741 ggtcagcaaa agatcttctt ctccaccctt aggagaaagt tgctttttta aattccggaa
     7801 acgcaattgt cccgtttcat ttttctcaac caacgacaat ccctctccat cggtgctcac
     7861 ccatgttttt ccttcgttat caatataaac aaaacgaacg gaattggagg gaagcgaggt
     7921 ttcgtcgttc gggttattgg tataaaactt ataactgccg gaacttaaat taatacagga
     7981 taatccttcc ttggttccta cccataaaca tttgttgcgg gtgtcctcgg ttatggaata
     8041 caccctgtta tcggcaaaac catttccggg gggacgaaat tgcaccggaa caaaaacagc
     8101 aaggcgaaga ttatcggata gttcaagcct gtacaaaccg tccactgcgc ctacccatac
     8161 acgtaaatga gcgtctacat acaaggatag aacggaatta ttcccaatcg cattcggatt
     8221 gtttgctttt ctgtcataac gaaaaacaag attgtttctc cggtcaacac ggttcaatcc
     8281 ttttcgtgta cccacccaaa gtattccttg ctgatcttca taaatactcc aaacagtacg
     8341 gtcattaatt gtattgatat catcaggaga atgattgatt tgcccgaata cctgcttaag
     8401 aggatcaaac ctgctcagac cttcatttgt acctatccag atcgatccgc tgcggtcttc
     8461 gtaaaccgat tgcaccagat tatccgcaag tgatccatcg tctgaatctt cgtgtttgtg
     8521 attgataaaa tatccgggac ggtcaagatc cataatatca attccgctgc ctgttgttcc
     8581 aacaatcatc aattgatcgc gggtaatgtg caaacagcgt acagagttat tggaaagcga
     8641 atgcggcgac gaataatcat tgatgaatcg cttacacaca aattcatctc cggatggtgt
     8701 catggaaacc aaaccggaat ttgttcccac ccaaattgtt ccctttttat cttcggtaat
     8761 acaccagatc acatcgtcga taagcgggtt cacatacctg tcgaagcgtt catgtgtctt
     8821 gttgtacatc agcaaaccct gaccggaaga acctgcaaaa acccttccct tactgtctga
     8881 ataaacacat tgaacattac gtgcttctac acctttaaaa acataggtaa taacacggcc
     8941 tttttcttta tttaatttat ttaccccgtt acccatcgta gaaaaccaga tgtttccttc
     9001 cgaatcttcg gtaatatcgg ttactgtgcg gttttgcgga cctaaatcgt atcgtttgaa
     9061 ggtgtttgtc cgcggatcaa atcggttcac ccctccgccg tttgtagcga gccagatgaa
     9121 accctctttg tcctcgtaca aggcgtgaat aaaattatta ctcaggctct gctggtctgt
     9181 aggatccttt ttataaataa cgatccgcgc cccatcatac ttgttcaacc catcctgagt
     9241 ggcaatccag ataaagccct gacggtcctg aattaaatca ttgacaagac tctgtgaaag
     9301 tccgtcgttg atgtccatat tatcaaaccg aacagcttta ttctgtgccc gcagggaaag
     9361 aaatgatacc gataacagta aaagcgaaaa cagtcggaac ataggacgaa gatctgacca
     9421 aaaataagaa ttaatagacc ttaatgaaca cactgcaaaa agagagcgtc ctgcttgtgt
     9481 cagacacgca caccaattac gagaatatcg tcgacctgct cataattgcc ccgccatttc
     9541 tcaatgctat cgtgaagtaa tttccgttgt acatccattg ttttatcctt catcccaatc
     9601 aaaagatcac ggaactgacg atacatgaat tttttaccct gcataccgcc aaactgatca
     9661 gcaaatccat ccgagaacac atagatcgca tctccctttt tcaattgcat tttatgattg
     9721 gtatacttac gcgcaccggg ttcaaacgca ccgatggcaa ttttatctgc tttcacctgt
     9781 aacaattccc cgtcgcgcac aatgtacaag gggttatttg ctccggcgta ttccaactcc
     9841 agtgtgtcgg tgtgcaatga gcaaaacgca agatccattc catcacgaac ggaattgttt
     9901 tccttatttt tatttaaagc ttctgaagaa agtttgttta aatcgtcaag aattcgcgcc
     9961 ggttctgtat gcccatgctc ttttactgcc tgattcaagg cgttcgatcc aacaagactc
    10021 ataaacgcac cgggcacacc gtgccctgta caatccaccg ctgcaaacaa taccttgttc
    10081 tcccttttgt agaaccagta aaaatcgccg gatacaatgt ccttgggttt aaacaatacg
    10141 aaggagtcag gaagcaactg acgaaccatc atatcgggag gcaatatgga ttcctgcagg
    10201 cgttttgcat aacgaatact atcggtaaca tccttataga gttcctcaag tttctggcgt
    10261 tgtttttcaa tttcctcttt ctgacgaaca acctcttctg ttcgttcgat tactttttgt
    10321 tcaagtattc gttcattctc cgcgaggtca tcacgcattt ttaacagggc atggcccagt
    10381 acgtcttgat cacttagcgg ccgatactgg tagttaaaat taccaatacc cacctgacgc
    10441 gagaaatctg ttgtgcggcg aaaaccatcg atcagattat tcatcgcaat ggtcatctct
    10501 ccaatttcat catcgcgagg ggagatctgt tcttccggaa tgattccttt acccaacatt
    10561 tgaagactgc tcttcaaacg agcaacagga cgtacaatag agcgagaagt aaacgttgcg
    10621 ataagtattg atccaacaac aagccccaca cccgaaatga gaagaagata aatcaggaat
    10681 ttataagaac ccattgttgc attactcgaa accacctgtg catcccttgt gttcttcaag
    10741 cgcatttcct gcatcctttt aagacgattc agggacatca taatcatttt cacttcccta
    10801 tccacatcac catccgtttc aaccagaaaa cgcgccatca gggcacgttc tgcatctgaa
    10861 taatcttcga aggtgacgag cagttcctga atttgggtat ataatcctgt cagctgctca
    10921 atgttgttga atacagaatc gatgatggtc acctcctcct gaggccagta ggtggtaaac
    10981 tccttgatct ggccttttac ccttggcagc tctttgttca ttaaggcaac gagacgcagt
    11041 ttatccggat tatcatccct cgactgttcg gttgcccatt tagtaattag tgcttttgat
    11101 tcctgaatca gattgctgag ttcaataacc aggttcaccg aaggcgtttc gattctggtg
    11161 atttgttcgt tcagtttttt cgactcttcg aaaatagaaa ggccggaaat tacattctga
    11221 acggtaaccg aaaagataac tgtgataaag aaaataacga ctccgaaacc taatccgatt
    11281 cgtcgtccga tattaaacct gaacttcata atggggtgtc tggtttttgg ttatttatcc
    11341 tcaccctcct gaagtttctt tttcaggtct tcccaatact taaatttatc cggttcattc
    11401 aactgatagt gaatgccgct cagcccttct gcgatttcga gcggattaat atccttgcag
    11461 ttttgaagct cccatgcttt gtacatgtgt ggcttcgatt tctggaagag ctctacacaa
    11521 atctcctgtg atttgatgat ttcttccaat ggcgttgtag gatcaagatt aagcaagatc
    11581 tcaacacctt tattgtaata caatattccg atctggtaat gtgccagaca gttgttactg
    11641 tcaagttcaa ttactttctg ataaacttct atcgccttat tgaaaaacac atcggcacct
    11701 ttttccattc tgttgaattt ctttaccaaa atggttgcaa aggcattgta atattcaaca
    11761 tccgatttca tgaaatcagt tgccggagca aaacgcttca tctgacgttt atattccaaa
    11821 tagaattttt ctgattccat gtagttggtg gtgtccatca atcggacagc atcattgaag
    11881 taagaaatta caagactgcg aattgaacca ttattgcgct gcgtaaagtc acctgcctta
    11941 tcgagttcat ttgatttgta gaacgactca attgcaattg cacgggccgt tgagtttcta
    12001 ttttcttttt gtgcgatttt gtaaatgtct ttataaatga acccacgtaa atgccatgta
    12061 aaaggatctg cagcctcacc tgaagcaaca gaagcactat cgataattcg tgcggcctta
    12121 tcccactcgt taccttgata ggcctgaaga gcgcgctccg ctacaggacc ctgcgcaaaa
    12181 gcagtggatc ctgagatcag cataaatagc gctatgtaaa ttatcctctt catattattc
    12241 aactcttgcg gccagatttt caagttttga cccaattatc agcttacttt ttgatgcgtt
    12301 tgatttactt agttcgtagg attgtttatt gtctttgatc acgaagctga taactgctcc
    12361 ctctttcagt ttgccttctt tttccgatac aatcagggtg ctgcttgatt taaatttctt
    12421 tgcaaggtcg gctactttat cacttacttc cggtgcaacc acaagtatat gacagggtgt
    12481 aatactgtta tagctttgaa aactgcgaat cttaatagcc tgacttccca cggagcgatt
    12541 agagtattta ttggttaatt catcatacag cggtgattcg ccgtagaccc caataataaa
    12601 gtctcccttc ttaaattcct taggccaatc cacgagcgta gcaaattgat acacgaataa
    12661 tgcttttaca gttttatggg tgtcctttgg atcgccggac agcatatcca tagcaagtgt
    12721 cggaaagacc aataatgcta ttaggaacca cctcttcatg cttctacttc tggatgtagg
    12781 aatttgtttt gcaatataac attaattctg tgctgttcat cacaattaat gctctaaaaa
    12841 ccgtgccata agaggcgttg cctagtaatt ctgcttaagc ataggctttg gaagggcgtc
    12901 attaccctta tttttggatc gttcgcggcg gttttttcgc tcgattttac gctgactttg
    12961 cctgaattcg gggtcgtgac gctctttgcg ttccttttta accctttttc gctcttcctt
    13021 cgatcggaac aacttcagtt tctttttgcc atagccttca ccggcttttg cttttttacg
    13081 tttaccctta ccagaaaagg gatccgactt ttccacactt tttttccggg ttgaggtttt
    13141 ggtactgaac gcgtctttcc ctcctcccga agaaggtttc tttccccttg ccacaaacga
    13201 atcctcagtt accaccctgg gtttacgtcc ctgtgatttg aaactgtcat cgcctgagcc
    13261 gcctgaaggt tttcttcccc tggatttaaa actatcgtcg cctgagccac catttgcctt
    13321 ccgccttctt gcagaaaacg aatcgctttc tttacttttc tttttgggtc gtgaagatgc
    13381 cacaaaatga tcagccggtt ttgcattgtc ctttttgcga ctgcgggcta caaaagcgtc
    13441 tttatcgcgg ttggagcgct tcttattggg ttttgcagta aaggttgagc gggttttctg
    13501 ttgctttttc tttgatcgcg aagaattata ggtttctttt ccatctgata ctttcgatcg
    13561 tttgccggcc tgcgcgttca gctgaccgga caaagaaagc gccaggaaga aaacaaataa
    13621 acctctgaat aaatacattt tcaataataa gtcttcagtg tacgaaataa gccttctaca
    13681 agttccgcca ttttttcgta atcgagggta tcccaggtat ccgttcgctt atggtaattc
    13741 ttgttacgca taaaagcaga atctgttaac ataatggcct taaaaccgaa taaccaataa
    13801 ttcagatgat cggaaaaatc aacaccttcc actcttgagg gtgcaattaa catttcacaa
    13861 ggcaggtttg aatatttatt tattcccttc ttaaaaaccg aaccaagatc cttcatccct
    13921 tttttcccga caaccgcaat aaaattacct ctgttgggat accataactt catgatattg
    13981 gccgggtatt gctgagagcc tttttcgtcc gaataaaaac cgatcatttc cagacaaacc
    14041 atcgcctcaa catttacttt tcgatcagca agatccttgg cgtgcacata acttcccata
    14101 ttaactgttc tgaaatgagg gggttcttca agggtataaa aaaccatttc aatacaatgc
    14161 ttcaagcggg gtttttcaat gttcagtctt ctgctcaact ccaataatcc ggcaacgcca
    14221 ctggcattat catccgcacc tggcaggttc atacacacat cataatgcgc accaattaca
    14281 agccgaggca aagtggtatc accataaaaa gcaatgatat tgtgtacacg aagcgaatca
    14341 acgcggtact cttgtttcct ccagccttgt cctattgaat caagcgagcc ccggatatag
    14401 gccgatgctt catcaaggcc ggattgaaaa tttacacttc gcggtttttt tgtgtgactg
    14461 agttgataaa cgtgttgttt caactttacc ggcacatcta ttccatagcc acaggagcta
    14521 atgaaaattc caaaagcaaa aaaaagtacc ctgaaggatc ttccttgcaa agaatcaggc
    14581 tttattagca ggaccaccag cggtattggc agtaagcgtt tgaatatctc tatcgaacaa
    14641 atacatgccc cccttatcgt ttccaatcat gcgtaattta tccatgatcg tacgggcaag
    14701 cgcttcttct tcgatttgtt cagaaacata ccactgcata aaattgtgtg ttgagtagtc
    14761 tttctccctc agacaaacat caactacttt attgatctcg gctgaaacaa ggatttcatg
    14821 ctgcaacaat ttttcaaaaa caaaatgaag atccttgaac gttttcggag gttgagaaag
    14881 cgcaggaatg actgcatgtc ctcctcgttc atttacat
//
LOCUS       DAOMYG010000017        13734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1498_length_13734_cov_12.897361, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000017 DAOMYG010000000
VERSION     DAOMYG010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 13734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13734
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1498_length_13734_cov_12.897361"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1588)
                     /locus_tag="PK610_01270"
     CDS             complement(<1..1588)
                     /locus_tag="PK610_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRJ34581.1"
                     /translation="MLRIFIVVCLLCLLPEQVFSVSLVPYRLTSGKWVLHDRDLKTHT
                     EGEFNSLTACSGPVIIAQRDDQWSLIGSNGDVLIPFGAYRKIREMSNGCIEACGKELI
                     DLYSRTGQRLGKRSYQWIELFRDHPQKLVVKTAEGKVGIVNTDNPDTEERIFDFAPVH
                     LDKEHIRIRVNKNGEERQGVCDNQFREIVPSVYDEAGVFRWRYYYGWNRGGDMNIFDK
                     SGKLIWKGKAGNEPQTIYPDFFIYSIDGDEDLVRVESRKTGDAILCDDHDIYVEEKQL
                     FICSYKGNSVMFFSNGKKKTFPNTSFSRMRSDEYFIAREGKGNSSTGKTALIDCNGTK
                     ILDYEQRQIAELNETFLVSTHVTSERKRNFSLINIKSGKTIIGPTPDEIHLLGERLYC
                     ITNGGTTKIYTLNGNKLQGEFDGVTAHDMVNQAIGYNTAFVVHKAVFSGAKENKKYLK
                     GLIGSDGRMVISVEFDEIRPLENRYDNARKTPRLVATKINEHDNRIRVSVLLDEKLKI
                     LTQQPASHFYVLHSDYICFGN"
     gene            complement(1640..2134)
                     /locus_tag="PK610_01275"
     CDS             complement(1640..2134)
                     /locus_tag="PK610_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34582.1"
                     /translation="MKMQIRYQEKKELSVEVGAYNKEAYVLQLKYDHREILEKHIPEI
                     ESHFLNLIHSGVLQQSVAIVVAWKWFADFSEKLRDHFEMEDRFVFPYLLGKGTPCASE
                     SALEFMRNHCSFEEQLQEYILVMCKTLRPLESDMAYRMLIHKLERLEAVLVEHGEAED
                     LLMS"
     gene            2457..2530
                     /locus_tag="PK610_01280"
     tRNA            2457..2530
                     /locus_tag="PK610_01280"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2491..2493,aa:Ala,seq:ggc)
     gene            complement(2741..9715)
                     /locus_tag="PK610_01285"
     CDS             complement(2741..9715)
                     /locus_tag="PK610_01285"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like protein"
                     /protein_id="HRJ34583.1"
                     /translation="MKKITHFVAGILTIGFLAFTQTLEAQVNLGSGLVAYFPLNGNAQ
                     DFSGNGNNGTGAGTVNWVNDRFGNPNSAVGFGGTGSPGKITVPHSASLNFSTGASFAF
                     WVRVNSNVGTFGNGSTGAGGSQCLFAKEGDAGGGLWSLARITGGGLPHDIGVNGMTTL
                     TGTHTPYTTGTWVHYVVTMDASGHQVYINGVQTAFNTHPANFTAMVNRNLVLGRFNSN
                     WYPLNGHMDEFRVYNRVLTNDEITALATDDLGSITSTPSSAGPYCAGDALSVSYNATG
                     NSYLAGNVFQLQMSDASGSFSNPVIVATATATSGTYNTQVPTGTPSGAGYKFRVSSTL
                     PLINGTESAAMVVNGVLGDIPSSASFRYIGNVSGRNYYMSLNAQTWNNSSTLCSANGG
                     HLAHIPNQEVNDYLQASAPSSQISFIGLTDVVTEGLYIWQNNYAPSFTNWATGQPDNA
                     SNEDFVELRHSDGMWNDRNGTMTRNCIMQLNPAGNDLVICEGANIALGAAPLTGATYL
                     WTGPNSFSSTQQNPIISNASSVHTGTYSLVITKGGCSSSAQTVNVTVVSRPVDIGQTS
                     ALPSSLSSGLILHYPFNGNANDASGGGNNGTLQGGVTPIADRFGSLNSALALNGTTGH
                     ITVPSGAWFDGGPFTVSAWVRKSSNNNFSRLLDFGNGQANNNVVLAISSGTSGRPLAQ
                     FYQGTTGGAQVSSPVTVLTNNEWELLTYTYENNAGRIYIDGILVGQGTQTAPINVVRA
                     INYIGRSNWASDGYANARFDDFRIYNRVLSLNEIIALVAEQPTVLSVYAMPSTLCASA
                     TSQIVLKSSQPGISYQLQNAATSANIGTVQMGTGDSLVFVTGTIAATTSFQLVAVSSI
                     TNCSRILSPAVTVNVIPTPTAPVTTGDQVCNEGTMTLSATGAPGGANYNWYTAPTGGT
                     PLAGETGNSISTGLVDESVSYYVSYTDVNGCESPRTIVTGTVINPLNPPVDIVSGLIL
                     HYTFDGNVNDYSGNGYNGTVNGTNNYVNDRLGNANSALNTVSGSTPGNNFINSGNPAK
                     VQQLTNQVTISMWIRQTQTWFGSGDGYMPLVNKWDGTGMYTGLYMTNPSNMQNRVRWR
                     INGSLVLNSSQNVPVGEWHHIVCTYNGAQLRIYQNGVLTGTLNHTGNIPNTGVNLEMG
                     RQANGVGDITYRGDWDQVKVYNRALNLNEVQTLYNNESVAFANDPLCDGEDDLQLSTF
                     NFPGATYQWSGPNGFTSNVQNPPVITNADSATYSGVYTLLVTAQGCTSPPQQTTVTIY
                     QIPSAPSVVNDTVCGSGNAVLQASGAPTGASYAWYTVPSGGTPISGQTGSSLTLTGVT
                     TTTVRYVSIVRNSCEGPRTAVTAVYNNPVSTGLTVNGSSVCISQSQAAVTILASQTGV
                     NYQAFFGGNPVSAIVPGGGDILLSVNTSTLSTGNNTITIVASQAGCGNVNLTNTATVQ
                     IYAAPSATISASGSTDICIGQSVTLTASTGTSYLWNNGSVSNAITVSVAGNYSVTITD
                     ANGCSATSATVELVTNAVPNPVITPAGALSFCQGGSVVLNASGGTTYQWSTGATGNSV
                     TVSSSQQVTLTAFNGGCSAVSSPVDVIVIPAPSVGISASATSVCPGVSVTLNGTGAQS
                     YTWNNGVANGVSFSPATSQTYTVTGSDANGCTNTAQITITVLPGANATFSASTPTVCP
                     GADVQLTANASGLTQYDWYFNGSPLQMNGPSAITVSTPGDYELQVVDNNGCTGSFAMN
                     IGTGSTPTASISSTGTSFCQGSNLLITANLISGSVYQWYVNGNPVGGATVNGNTFSAT
                     TAGMYTVEITNSNGCSALSNAIALTTTAGPLASIQSSGTLVCPGDSVYLSADFVSGAT
                     YQWYFNGTPISGATLDHYYAVAAGTYSAEVTDGCTSVSNNITLTSGSAPSNAGSISGA
                     TSECSGESLMYSIASVSGATSYQWSVVPANAATIHSGQGTNSVLINTTNQNFTLSVTP
                     VNSCGNGGSSSLAVSVTNAFPCSGEILFGAYPTLVCQGNNVVFTNYTNSMLFPGFTPR
                     WNFGAGASPATANGNGPHTVTYSSTGQKTVTLEYVDMFGFVVDDLTKINYINVSAPVA
                     TGVISGNTTISCSSNLESYSVNATAGSSYQWSVPAGAVIVSGQGTSSVGVNWNGASGT
                     ISVIETNASGCAGTAAQLNVNISNPVLTSSISGSATVACTANGQTYSVTPTSGSTYLW
                     NVPAGVAIVSGQGTSSIVVDFSGSAGTITVTETNAAGCTGAPQTLLVNCTNDISENRI
                     LTARIYPNPTAGMFRLEWGSEGEVGQLQLYNALGAMVYSEMLGSGSDVNISILTPGIY
                     TGRLESAGSVYQFKVIKQ"
     gene            complement(9857..12418)
                     /locus_tag="PK610_01290"
     CDS             complement(9857..12418)
                     /locus_tag="PK610_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ34584.1"
                     /translation="MVLLLLRVTICFSQDGVRLLEKGRHLLSKIPDSTVWYANQALVL
                     SEKNNDFQTAAKAFTLLGEAYQAKSESALSVEHYLRAINLCEQYRLDTLIGSALNGLG
                     ITYYYINDLEKSEYYIRRAADAKLKVKDYTFYSVVLTNLAGIMFYKERYDDAIALLKG
                     AETKIKEANQEKYLASLYNSLGGAYQMKSAHPDSVLYYYLRSIEIASKWDLRENLITG
                     YHNLGEFYFRGKEYVKSIANLRKALQLSEDGGRDRYTLALYSTLSEVYFQIGEFVKAY
                     EMKSKELQLVKKINEAEKMRSIEELEISYQTRKREQELREQELALQASRLESERYKSR
                     LYLIVLISLLVILGLLWFVFYQIQKRRLNQQIVQEKSKLFENIVHEIRTPLTLIQGPL
                     QLIKKEVQQMPGGDKNLQLIEANAARLIRLVNELLDASKMEKGGYAPEHKTGDVGAMI
                     HTIVGSFYSEAGLTKATFEIVLSSPTMICTYPSNVLEKALNNIVGNAVKFGGKGANIR
                     VEAELVENKLVVSVSDDGPGIPAGDRDAIFTRFFRGVNGKKQSGTGIGLAFTKELLEL
                     YGGKIWFESPINGGAKFSFELPVSSISSTEEVQDVVNELPVLLVAEDDPQLLGFVRDV
                     FSSEYNIITVSNGEDAIRLVQERIPDVVLSDIMMPGKDGLEVLRQCKTDDLTAHIPVV
                     LFSSKNALQSKLDALQLGADAYVAKPFHPDELRYTIHNLLELRRKSQQQAIEDLRKSD
                     LPVEDRLKSKNEFVNKAITFIMDHLSDGSYSVNELASDLCLSRSQLHRKITAFTGQSA
                     THFIRMVRLEKSREFLASGKGNVTEVAYLCGFSSHSYFSRSYAEHFGHSPSQDMP"
     gene            complement(12581..13273)
                     /locus_tag="PK610_01295"
     CDS             complement(12581..13273)
                     /locus_tag="PK610_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HRJ34585.1"
                     /translation="MSIRVVIVEDEMLLAEDIATDLHGFGYEVAGIFISGEQCLDVIH
                     DLKPDVVVMDIRIKGSIDGIETARRINDILPTPIVYLTSNSDQATIKRLLDVFPSAFI
                     SKPYQKTDLMMAIEMAVRKGAASTETEKCLFIKASSGYRKIELAEILFLEAEGSYTRI
                     QTTSESHLVSYNLRYFEESINSTQFRRIHRSFIVNTQHVQTASSSQVSIGDKNIPVSK
                     TYSKEVLEWFGG"
     gene            complement(13270..13686)
                     /locus_tag="PK610_01300"
     CDS             complement(13270..13686)
                     /locus_tag="PK610_01300"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRJ34586.1"
                     /translation="MRTFTQRLTTNLCDVYGIDMKKIDIRILFPELRLDVDTAMPIAL
                     ILNETIVNAFKHSYPKHPDDFIISIDMKEEAGELVLLVKNNGDRVDPTVIQNSASFGF
                     RLIDLLTRQLRGTMEILDQQGFGFKFRFKEYKMVNG"
BASE COUNT         3851 a         3239 c         2791 g         3853 t
ORIGIN      
        1 aatttccaaa acatatataa tcactatgta gtacataaaa atgagaagcg ggttgctgtg
       61 taaggatttt cagtttttca tcgagcagaa cactgacacg gattcgatta tcatgttcat
      121 tgattttggt tgcaactagc cgtggggttt ttctggcatt gtcgtaacga ttttccaatg
      181 gacgaatttc atcgaattcc actgagatca ccattctgcc gtcactcccg ataagccctt
      241 tcagatactt cttattttcc tttgccccgg agaaaacggc cttatgaaca acaaatgcag
      301 tattgtatcc gattgcctga ttcaccatgt cgtgcgcagt gactccatca aattcacctt
      361 gcagtttgtt tccgtttagc gtataaattt ttgtggttcc tccattggta atgcagtaca
      421 atcgctcccc aagaagatga atttcatcag gagttggacc aataatcgtc tttcctgatt
      481 tgatattgat cagtgaaaaa tttcgctttc gttccgatgt aacgtgtgtg cttaccagaa
      541 atgtttcatt caattcggca atttgtcgct gttcataatc gaggattttt gtgccgttgc
      601 aatctattag tgcggttttc cctgtagagg aattgccttt cccttctctt gcaataaaat
      661 attcatccga acgcatacgg gaaaaggagg tgttaggaaa tgtcttcttt ttaccattgg
      721 aaaaaaacat taccgaattc cctttatagg agcaaataaa tagttgtttt tcctcaacat
      781 agatatcatg atcatcacac aaaatggcat caccagtttt tctgctttcc acccgaacaa
      841 gatcttcatc cccatctatt gagtaaataa aaaaatccgg atatatagtt tgcggttcat
      901 ttccggcttt gcctttccaa atcagttttc ccgacttatc aaaaatattc atgtcacctc
      961 cacggttcca gccataataa taacgccacc taaatactcc ggcctcatca tataccgaag
     1021 gaacaatttc ccggaattgg ttatcgcata ccccctgacg ttcttctccg ttcttattca
     1081 ctcttatgcg aatgtgttcc ttatcgagat ggactggagc aaaatcaaaa attcgttctt
     1141 ctgtatcggg gttatctgta ttcacaattc caactttccc ctctgctgtt ttcaccacca
     1201 gcttttgcgg atgatcgcgg aataattcaa tccattgata ggaacgctta cccaggcgtt
     1261 gacccgtccg agaataaaga tcaatcagct ccttgccaca ggcctcaata cacccattac
     1321 tcatttcgcg aatcttccga tatgctccaa aaggaattag aacatcacca ttactaccaa
     1381 tcaaactcca ttgatcatcg cgttgagcaa taattaccgg tcctgaacat gctgtgaggg
     1441 aattgaattc gccttcggta tgtgttttta gatcgcgatc gtgcaacacc catttaccgg
     1501 aagtaaggcg ataaggaacc aggctgaccg aaaacacctg ttccggcagc aagcacagta
     1561 agcaaacgac aatgaaaatc cgcagcatcc gattgtaatt tacaaaaaca gatgcttcaa
     1621 cgaaaaaatt aacaaactat taggacatca gcagatcttc cgcctcgccg tgttccacta
     1681 ataccgcttc cagtcgctcc agtttatgaa tcagcatacg ataggccata tccgattcga
     1741 gcggacgtaa tgttttgcac attaccagaa tgtattcctg caattgctcc tcaaacgaac
     1801 aatggttgcg cataaactcc aatgcacttt cactggcaca aggagtgcct ttccccaaca
     1861 ggtaaggaaa tacaaaacga tcttccattt cgaagtgatc acgcagcttt tcgctgaaat
     1921 cagcaaacca tttccatgca acaacaattg caacagactg ctgaagcacg ccggagtgaa
     1981 tcaggtttaa aaaatgtgat tcgatttcgg ggatgtgttt ttcaagaatt tccctgtggt
     2041 cgtatttcag ttgcaataca taagcttcct tattataggc tccaacttct acacttaatt
     2101 cctttttctc ctgatagcga atctgcattt tcatactcaa aaatttacag atgcaatgtc
     2161 ggtgatgcaa gcaggaatcg gaataccgta aacaaggtaa aagcgccccg aaatattcac
     2221 ttcagctggt taaaaagaga tgtgctgatt tctgtcaggt ttcaagacca atattacaac
     2281 acgctgaaat tcaagcccaa aacatcaggt ttcaatgtat atatatttat taaatttgcg
     2341 atctgttctt taaaagattg ggggattagc tcagtccaga cgcttacggt ctggatgcaa
     2401 gcactggctc tacttgatct ttgaatattt ttgaatacgt tcttttaata ataaatgggg
     2461 gattagctca gctggctaga gcgcttgcat ggcatgcaag aggtcatcgg ttcgactccg
     2521 atattctcca caattacaga atcccgcaat cgcgggattt tttgttttaa tccaactaca
     2581 taaagcgtcc ctatcgtacc ctcccaaaaa aaccaggaac atcgggatgg tcacctgtaa
     2641 ataaggaaag gaactgattc tgcaaaagag gaacattccc atctcaacaa aaaaaaccgc
     2701 ctcctctcgg agacggtcct ttttgcaagc gaaggaggaa ttattgtttg attactttga
     2761 actggtaaac agatcccgca ctttccaatc gaccggtata aattccggga gtcagaatgg
     2821 aaatgttgac atccgatcct gaacccagca tttcggaata caccatcgct ccaagtgcat
     2881 tgtacaattg cagctgacca acttcacctt cactgcccca ttccaatctg aacattcctg
     2941 cagtaggatt cgggtatata cgcgctgtaa ggatgcgatt ttcactgatg tcgttggtgc
     3001 aattcaccag caaggtttgc ggtgctcctg tgcatcctgc tgcattggtt tcagtaaccg
     3061 ttatagttcc tgccgaaccg gaaaagtcaa ccacaatact tgaggttccc tgaccactaa
     3121 cgatcgcaac tcccgcagga acattccaca aataggtaga tcctgatgta ggtgtaacgc
     3181 tgtacgtttg tccgttagca gtgcaagcta ctgttgcact tccgctgatt gatgatgtaa
     3241 gcacaggatt gctgatgttc acattgagtt gtgctgcagt tcctgcacaa ccggaggcgt
     3301 tggtttcaat cactgaaatt gtacccgatg ctccattcca atttactcca acagacgatg
     3361 ttccttgccc ggatacaatt accgctcctg caggtacgct ccattggtag cttgacccag
     3421 cagttgcgtt tacactgtag ctttctaaat tggatgaaca acttatcgtt gtgtttccac
     3481 ttatgacacc tgttgcaacc ggagcagata cattgatgta attgatcttg gtcagatcat
     3541 ccacaacaaa accaaacatg tcgacatact caagtgttac cgttttttgt ccggtagagg
     3601 aatacgtcac cgtatgcgga ccattcccgt tggctgttgc cggactggca cctgcaccga
     3661 aattccatcg gggtgtaaaa ccggggaata acatgctgtt ggtgtaattg gtgaacacta
     3721 cgttgttgcc ctgacaaacc aatgtaggat aagcgccgaa caaaatctct ccactacaag
     3781 gaaaagcatt ggtaacactt acggcaagag aagatgatcc accatttcca caactattga
     3841 ccggagtaac cgaaagtgta aagttctgat tggtggtgtt gatcaaaacc gaattggttc
     3901 cttgtccgct gtgaatggtt gcagcatttg ccggtacaac actccattga taggacgtag
     3961 caccacttac ggaagcaatg ctgtacatca gactctctcc ggaacactca cttgttgcac
     4021 ccgaaatact tcctgcattc gaaggagcag atccggatgt aagcgtaatg ttattggaaa
     4081 cagaagtgca tccatctgtg acttcggcag aataggttcc cgcagcgaca gcataataat
     4141 gatctaacgt tgctcctgaa atgggtgtac cgttaaagta ccactgataa gttgcgcctg
     4201 aaacgaaatc tgcagagagg tacacagaat cgcccgggca aaccagcgta cccgaactct
     4261 gaatagaagc aagtggtccg gcggtggtgg ttaatgcaat tgcattggac aatgctgaac
     4321 atccgttact gtttgttatc tccaccgtgt acattccggc cgttgttgca gaaaacgtat
     4381 ttccatttac tgtagctcct ccaacgggat ttccgtttac gtaccattgg taaacagaac
     4441 cggaaattaa gttagcagtg ataagcaaat tgcttccctg acaaaacgaa gttcccgtgc
     4501 tgctgatgga agctgttgga gtagatcccg ttccgatatt catggcgaaa gatcctgtac
     4561 aaccattgtt atcgacaacc tgcaattcgt aatcaccggg ggtagaaact gtaatggcag
     4621 aaggcccatt catttgcaaa ggcgatccgt taaagtacca atcgtattgc gttagtcctg
     4681 atgcattggc agtaagctgc acatcggcgc ccggacaaac tgttggtgta cttgcagaga
     4741 acgttgcatt tgctccggga agaaccgtaa ttgtgatctg agctgtattg gtacatccgt
     4801 tggcatccga accggttacg gtataggttt gagaagttgc aggtgaaaac gaaacaccat
     4861 tggcaacacc attgttccag gtgtacgatt gtgcacccgt accgttcagt gtaactgaaa
     4921 caccaggaca aacacttgtt gcgcttgcag aaatacccac cgaaggagca ggaattacaa
     4981 ttacatcaac cggtgaagaa actgcagagc aaccaccgtt aaacgcagtt agtgtaacct
     5041 gttgcgaaga agaaacagta acagaatttc cggtagcacc ggtgctccac tgatacgtag
     5101 ttccacctga tgcattcaat accacagaac caccctgaca aaaactcaaa gcgcctgcag
     5161 gtgtaataac cggattcgga actgcattcg taacaagctc aaccgtagca gatgttgcgg
     5221 aacaaccgtt tgcgtctgta atggttacag aataatttcc tgctacagaa acagtaattg
     5281 cattggaaac agaaccattg ttccacaaat aggaagtgcc tgtacttgct gttaaagtca
     5341 ccgattgtcc gatacaaata tcagtagatc cgcttgcaga aatggttgca gaaggtgctg
     5401 cataaatctg aacagttgct gtattggtaa gattaacatt tccacagcca gcctgggaag
     5461 caacaatagt aatcgtgtta tttcctgtag acagagtgct tgtatttaca ctgagtaaaa
     5521 tatcaccacc gcccggcaca attgcactca caggattccc tccgaaaaag gcctggtaat
     5581 ttactccggt ctgagatgca agtatggtaa ctgcagcttg cgactgactg atacaaactg
     5641 aacttccatt aactgtgagt cctgtagaaa ccggattatt gtacactgca gtaacagccg
     5701 ttctcggtcc ttcacaactg ttgcgcacaa tacttacata acgaacagtg gttgttgtaa
     5761 caccggttaa agtaagcgac gatcccgttt gtccggaaat tggtgtcccg ccgcttggca
     5821 cggtgtacca tgcatagctt gcacctgtag gagcacccga ggcctgcaat accgcatttc
     5881 cgcttccgca tacagtatcg tttacaactg aaggtgcaga aggaatctga taaatagtca
     5941 ctgtagtttg ctgaggagga gaagtacagc cctgggcagt taccaataat gtataaaccc
     6001 ctgaataggt agctgagtca gcatttgtga ttacaggagg attttgaaca ttcgatgtga
     6061 aaccatttgg tccactccat tgatacgttg ctccggggaa attaaatgtt gaaagctgaa
     6121 gatcgtcttc accgtcacaa agcggatcat tggcaaaggc aacactttca ttattgtaaa
     6181 gtgtttgtac ttcattcaga ttgagtgcgc gattatagac ttttacctga tcccaatcac
     6241 cacgataagt aatatcacca acaccgtttg cctgtcttcc catttcaaga tttactccgg
     6301 tgttcggaat atttcctgta tgattcagtg tacctgtaag cactccattc tggtaaatgc
     6361 gcaactgtgc tccattgtaa gtacagacaa tatggtgcca ttcgccaact ggtacatttt
     6421 gtgaactgtt aagcaccagt gatccgttaa ttctccagcg aacgcggttt tgcatattcg
     6481 acggattggt catgtacagt ccggtgtaca ttcctgtacc gtcccactta ttaacgagag
     6541 gcatatatcc atcgcccgaa ccaaaccagg tttgtgtctg acgaatccac atcgaaatgg
     6601 tgacctgatt ggtgagctgc tgaacttttg cgggattccc tgaattgatg aaattatttc
     6661 ccggtgtact tcccgaaacc gtattcagtg cagaatttgc attgcctaac cgatcgttca
     6721 cataattatt tgtaccgtta actgttccgt tgtatccgtt tccgctatag tcattcacgt
     6781 taccatcaaa agtgtaatgc agaatcaatc cgcttacaat atccaccgga ggattaagag
     6841 gattgatcac tgttccggtt acgatggttc tcggactctc acatccattg acatcggtat
     6901 aggatacata gtaagaaacc gattcatcta ccaatcctgt tgagatcgaa tttccggtct
     6961 cacctgcaag aggggttcct cctgttggtg cagtatacca gttgtaattg gcgccaccgg
     7021 gagctccggt tgctgacagc gtcattgttc cctcattaca cacctgatca cctgtggtta
     7081 caggagctgt gggtgtaggt attacattaa cagtaacagc aggactcaaa attcttgagc
     7141 aatttgtaat gctgcttacc gctaccagtt gaaaggatgt tgttgctgca attgttccgg
     7201 ttacaaaaac aagcgaatca cctgtaccca tttggacagt gccgatgttt gcagatgtag
     7261 cagcgttctg caattgatac gaaattccgg gttgggaact ctttaaaaca atttgcgatg
     7321 ttgcactggc acataaagtt gatggcattg catatactga taaaactgta ggttgctctg
     7381 ctacaagcgc aatgatttca ttgagagaaa gaactctgtt gtagatcctg aaatcatcaa
     7441 agcgtgcatt tgcatagcca tccgatgccc aattgctcct tccgatataa ttgatggcgc
     7501 gtacaacatt tataggtgct gtttgcgttc cttgtcctac aagaattcca tcaatgtaaa
     7561 tgcgacccgc attgttttcg taggtatagg ttaacaactc ccactcatta ttggttaaga
     7621 ctgtaaccgg acttgaaacc tgagctcctc ctgttgttcc ctgataaaac tgtgcgagtg
     7681 gtcttccgct ggttcctgaa ctaatagcaa gaaccacatt attgttggct tgcccgtttc
     7741 caaagtcaag gagtcggctg aaattattgt tggaactttt ccgaacccat gcactcacag
     7801 taaaaggacc gccatcaaac caggctcccg aaggaacggt gatgtgaccg gttgttccat
     7861 ttagtgcaag agcagaattt agtgatccga aacgatctgc aattggtgta actccaccct
     7921 gaagcgttcc gttatttcct ccaccactgg catcattggc gtttccgtta aagggataat
     7981 gcaaaatcaa tccggaagaa agagacgaag gcaatgcgga agtttgacca atatccaccg
     8041 gacgagaaac aactgttacg ttaaccgtct gtgccgaaga agaacatcct ccttttgtaa
     8101 ttaccagcga ataggtcccg gtatgtacag aggacgcatt gctgatgatt ggattttgct
     8161 gcgttgagga gaaactgttt ggtcccgtcc ataaataagt agcacctgtt aatggcgctg
     8221 ctcccaacgc aatgttcgct ccttcgcaaa tcacaagatc atttccggca ggattgagtt
     8281 gcataataca atttcgtgtc atcgttccat tccgatcgtt ccacatacca tcactgtgac
     8341 gcaattcaac aaaatcctca ttgcttgcgt tatccggctg acctgttgcc caattggtaa
     8401 aggaaggtgc atagttgttt tgccatatat acaatccttc tgttacaaca tctgttaatc
     8461 caataaatga aatttgcgat gatggtgctg aagcctgcaa gtaatcgttt acttcctgat
     8521 tgggaatatg tgcaaggtga cctccattgg ccgaacatag tgtggacgag ttgttccagg
     8581 tttgggcatt caaactcatg taataatttc ttccgctcac atttccgatg taccgaaaag
     8641 atgcagaaga aggaatatct cccaatacac cattcacaac catggctgcg ctttcagttc
     8701 cgttgattag cggaagcgtg ctgctcaccc ggaatttata tcctgcaccg ctcggtgtgc
     8761 ccgtaggtac ctgagtatta tatgtgcccg aagttgctgt agcagtagcc acaattaccg
     8821 gattggaaaa acttccgctt gcatccgaca tctgcaactg aaacacattt cctgccaggt
     8881 aactgttacc tgtggcattg tatgaaacac tcagcgcatc ccctgcacaa tacggtccgg
     8941 cagaggatgg agtacttgta atacttccaa gatcatcggt tgccaatgca gtgatttcat
     9001 cattggtaag cacccgattg tacacccgaa attcatccat gtgtccgtta agcggatacc
     9061 agttgctgtt gaatcgaccc aacaccagat ttctgttcac catcgccgta aaattggcag
     9121 gatgcgtgtt gaaagcggtt tgcactccgt tgatataaac ctgatgtccg gatgcatcca
     9181 tcgtaacaac ataatgcacc caggttccgg tagtataggg cgtatgcgtc ccggtgaggg
     9241 ttgtcattcc attcactcca atatcgtgag gcaatccccc gcctgtaata cgtgcgagtg
     9301 accataaacc acctcctgca tctccctctt tggcaaataa gcattggctg ccccccgcac
     9361 ctgttgatcc gttaccgaat gtgcctacgt tactgtttac ccttacccaa aaagcaaagg
     9421 atgctcccgt actaaagttg agactggcag aatgaggcac cgtaattttt cccggactcc
     9481 cggttcctcc aaatccaaca gcagaatttg gatttccaaa tcggtcgttt acccagttta
     9541 ccgtaccggc acccgttccg ttatttccgt taccggaaaa atcctgtgcg tttccgttaa
     9601 gtggaaaata cgccaccaaa cccgacccca ggttcacctg ggcctccaag gtttgggtaa
     9661 acgccagaaa cccgatcgtc aggattccgg ctacaaagtg tgtgatcttt ttcataagca
     9721 tgttttagct cgtgcagacc aaagatcatg cgcggtgcca aaaagcttat tcaatcatgt
     9781 tgcaagcttg tatactattg ttgcaatgtt gcattatcac gttaaaccac tgatattcaa
     9841 cagtaaaaaa ttaagattat ggcatatcct gtgacgggga atgcccaaaa tgttcggcat
     9901 aggaacgcga aaaatacgag tgtgaactga atccgcacaa atatgcaacc tctgtaacat
     9961 tgccttttcc ggaagcgagg aattccctcg acttttcaag ccggaccatc cggataaagt
    10021 gggtggccga ctgtccggtg aaggctgtta ttttacgatg caactggctt ctactcaaac
    10081 acaggtcaga tgcaagctcg tttaccgagt aacttccgtc cgacaaatga tccattataa
    10141 aggtaatagc cttattaacg aattcgtttt tactcttgag ccggtcttca accgggagat
    10201 cgcttttgcg aagatcttca atagcctgtt gctgactttt ccttcgcaac tctagcaggt
    10261 tgtgaatagt gtagcgaagt tcatccgggt gaaaaggttt ggcgacatag gcgtctgcac
    10321 ccagctgcaa tgcatcgagt ttggactgaa gtgcattttt tgatgaaaac aaaactacag
    10381 ggatatgcgc tgtcagatca tccgttttgc actgcctcaa cacttccagt ccatcctttc
    10441 cgggcatcat aatatccgac aatacaacat caggaattcg ttcctgaaca aggcgaattg
    10501 catcttctcc gttcgaaaca gtgattatat tgtattcaga agaaaacaca tcacgaacaa
    10561 atcctaataa ttgcggatca tcttcggcta ctaacaatac aggaagttca ttgacaacat
    10621 cctggacctc ttcggtagaa gaaatactac ttacaggaag ctcaaaagaa aatttagctc
    10681 ctccgtttat tggtgattca aaccaaattt ttccgccata caattcaagc aactcttttg
    10741 taaatgccag tccgattcct gttccacttt gcttttttcc gttcactcct ctaaaaaaac
    10801 gtgtaaaaat tgcatctctg tcgccggcag gaataccggg tccatcatca gaaacagaaa
    10861 caaccaattt attctccacc aattctgcct caacacgaat gttagcccct tttcctccaa
    10921 acttcactgc attgcccaca atattattaa gggccttttc caaaacattc gatgggtagg
    10981 tacagatcat ggtgggtgat gacaaaacaa tctcaaaagt tgctttggtt aacccggctt
    11041 cagaataaaa agaaccgact attgtatgga tcattgcacc aacgtcgcct gttttatgtt
    11101 cgggagcata tccacccttt tccatttttg atgcatccag caattcattc acgagtcgga
    11161 ttaaacgtgc tgcatttgcc tcaatgagtt gtaaattttt atctccacca ggcatttgct
    11221 gaacttcttt cttgatcaac tgaagcggcc cctgaataag agtaagcggt gttcggattt
    11281 cgtgaactat attctcaaac agcttcgatt tctcctgcac aatttgctgg tttagcctcc
    11341 gcttttgaat ctggtaaaac acaaaccaca ataatcccag aatcactaaa agtgaaatca
    11401 acacgatcag atacaacctg cttttatagc gttctgattc gagtctgctt gcttgaagtg
    11461 ctagttcctg ttcgcgcaat tcctgctcgc gcttgcgcgt ctgataactg atctcgagct
    11521 cttcgatact tctcatcttt tcggcttcat tgatcttttt gaccaactga agctccttcg
    11581 atttcatttc atacgcctta acaaattctc ctatttgaaa ataaacctca gaaagtgtag
    11641 aatacaaggc aagagtatac ctgtcccgac ctccgtcttc agacaattgc agagcctttc
    11701 tcagattcgc aattgatttt acatactcct tcccgcggaa ataaaattcc cccaggttat
    11761 ggtaaccggt aatcagattt tcccggaggt cccatttcga tgctatttca atgcttcgca
    11821 gataataata caacaccgaa tcaggatgcg ctgatttcat ttgataagca ccacccaggg
    11881 agttatacaa cgaagcaagg tatttttcct gattagcttc cttgatttta gtctcggccc
    11941 ccttcaataa tgcgatggca tcatcatagc gctccttgta aaacatgatt ccggcaaggt
    12001 tggtcagcac cacgctgtaa aatgtataat cttttacctt cagtttagca tcagcagccc
    12061 tgcgtatgta atattccgat ttttcaagat cgttaatgta ataataggta atccctaatc
    12121 catttaatgc agaacctatt aaagtatcaa gccggtactg ttcgcacaag ttgatcgcgc
    12181 gcagataatg ctccaccgac aaagccgatt ccgatttggc ctgataagct tcaccaagaa
    12241 gcgtaaatgc cttagccgct gtttgaaaat cattattctt ttcactcaaa accagagcct
    12301 gattggcata ccaaaccgta gaatccggaa tcttgctcaa caaatgacgt cccttttcaa
    12361 gcaaccggac tccatcctga gaaaaacaaa ttgtcaccct caataaaagg agaaccatcc
    12421 aagtccatct gataattaga ttcatatgcc taaaataggc aatatccaga ccaaaatatt
    12481 ctgaatagac tatttgtcag aaacacatcc accgaatgta ggattgtcgg tcgtttcaaa
    12541 accaaaccat tgggcccaat ctgatcaata acaaaagaac tcagccccca aaccactcta
    12601 acacctcttt tgaataagtc ttgctaacgg gaatgttttt atccccaatg gaaacctgcg
    12661 atgaagaggc tgtttgtaca tgttgggtgt tgacaataaa ggagcggtga atacgcctga
    12721 attgggttga gttgatggat tcttcaaagt acctgagatt gtacgagacc aaatgcgatt
    12781 cggaagtggt ttgaatgcgg gtataacttc cttctgcctc tagaaaaaga atttcagcca
    12841 attcaatttt ccggtatccg cttgaagctt taataaaaag acatttttct gtttctgttg
    12901 aagccgcccc ttttcggaca gccatctcaa ttgccatcat taaatctgtt ttctgatagg
    12961 gcttactgat aaatgcggaa ggaaaaacat cgagtaagcg ttttattgta gcctgatcag
    13021 aattggaagt aaggtaaaca attggcgttg gcaaaatatc gtttattcgt cgcgctgttt
    13081 ctattccatc tatagatccc ttgatccgaa tatccatcac caccacatcg ggttttaaat
    13141 catggatcac atccaggcat tgttcaccgg aaataaaaat acctgcaact tcataaccaa
    13201 aaccgtgcag atctgttgcg atatcttctg caagcaacat ttcatcttct acaatcacaa
    13261 cgcgaatact catccgttta ccattttgta ttccttaaac ctgaacttaa acccaaatcc
    13321 ctgctgatcc aaaatctcca tcgttccgcg aagctgacgt gtaagcagat cgataaggcg
    13381 gaacccgaaa gaggcggaat tctgaatgac agttggatct acacgatcac cattgttctt
    13441 aacaagcagc accaactctc cggcttcttc cttcatgtcg attgatataa taaaatcatc
    13501 aggatgtttg ggataggagt gtttgaatgc atttacgatt gtctcattca gaattaatgc
    13561 aataggcatg gccgtatcta catcaagacg tagctcagga aataaaatcc ggatatcaat
    13621 tttcttcatg tcaatcccat acacatcaca caaattcgtt gttaaacgtt gagtaaacgt
    13681 tcgcatttcg atgaatgaaa aatgggtgct gaggcatcat cagcaccctt gtgg
//
LOCUS       DAOMYG010000018        13432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1538_length_13432_cov_9.689093, whole genome shotgun sequence.
ACCESSION   DAOMYG010000018 DAOMYG010000000
VERSION     DAOMYG010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 13432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13432
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1538_length_13432_cov_9.689093"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..811
                     /locus_tag="PK610_01305"
     CDS             71..811
                     /locus_tag="PK610_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34587.1"
                     /translation="MRTTFFSSLVLSLMIFSVSPVFASWNAATLEQYEEHCKKISENY
                     ERLKAYSVLIQHSSFQGQNENSPLADQSEGFIKKEGIKFHSYLSGVRSVQNERIRVSV
                     DTSQKILVISEPEDITKNEVVSNNWKVTRKNIKGCLQKKIQDKTVYRIECISGPVFGI
                     EMIVNATNLIDEITILYRSEYFSEQLNKSVRPMLKIKYSQFIENPRFEKNDFSTEKYI
                     TIDKKGQIIPTQEYFSYKLIDTRYSTAQ"
     gene            836..13417
                     /locus_tag="PK610_01310"
     CDS             836..13417
                     /locus_tag="PK610_01310"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HRJ34588.1"
                     /translation="MMKKISYSGLLIAFVVLFTNVNAGHLEFSNRIFSVPAVSNILVT
                     DQLEIEVNPKTGSFTDYKVSNILTLGVDHHYSEFFATAMEVKVKFSLSQYNSANSLVG
                     TSSKYLTIKYDPFGGAYQDKSSFLFSNAYRFVAEIDSIYVNGVPKDSLPGLLFVEGYT
                     NIDRFYNFSSAATTPIVLNALTTSEVDCDGADDHITVSWPAVTGAEEYHLEWSFVNDY
                     NDSLTFKPASALTLDFRNNATRVSTKAENYTIPLLFEHGYLAFRVRPVGRDHSSPDNY
                     IFGVWSLPEKVKASDLDTSVVHVIFPHEANKNWQVTTTFAEEGKKKEVISYFDGTLRN
                     RQTVTRINSDNHIIVGQTIYDHQGRPAVTVLPVPVQDSVCDNSFVDHSLKYYPNFNLD
                     DTLNLYGRNDFDLDDPDSACAANAAPMSVTSGSSNYYSNDNPNQNGYQSYVPNSEKYP
                     LVQVEYTPDNTGRIRRQGGVGPEFQLGSGHETFYYYGQPNQIQLDRLFGSEAGDAAHY
                     KKNVVIDPNGQASVSYLDQEGRVIATSLAGDTTQNLLALDSENGAAVQLTIDLFSKNS
                     NGVSNTNVVTQDSTGIKFSTQLLVAYSSNFEFNYSIEVDTVDDACLKDNICFHCVYDL
                     VISIHDECMQLVYSTDTIIGHFTMEDTLIVFDTLCTDPTALNFDRNFTLALEPGNYTV
                     YKHLRINEAAMEHYLNLYLDTTYNSCFKTLAEFQEEYLSNIDSASCQISCESCIEALG
                     DRDEFVASGNGTAFEWDRRYEECRLMCTDTEEPCEAELMMMLGDVSPNGQYGQYLTGS
                     GSISPTSFPLSVFNQTNKLPESWNTPGQAYWRKPKIELNETVYPYYVDEDNQRSKVYI
                     VPNGNNYIPAVSDTSSAYVFTDNQGGKYTWPENLSDVADFIDLWKTSWSRSLVYLHPE
                     ICYYQTCLSYSEKAIDTNSFSSHSFDVLLRNTNSFYQAKNAGLIKSNYASFPDPNDRV
                     TDWFINQSGTTIAWDPFVVYNTGYGPALEQVFDTYVTYGGNSYSMVEIAALTARCGGL
                     TGINPSGTCLLFGSDMYPGGPAALNDSIRNLEWNNLRSFYLSKKLIMQSNYADNIALN
                     DCEAVNDCIGNQNFNAVESGMFSGGGFTNSPWFDGSQPCSVHNYQYYLNKISRFPDPS
                     DMEHIVGSSDVHDVNYQHYLQTGQCPLAVHMQEMLGALAHADVLDSTDIQMMQFPGFS
                     TWFLDFSGYSVNSSINTFDLYWNTTSAGNTISITLTNGSGGAAICTGALVKDTSIASW
                     DDISFFTGIQYTHSISNQHYFTMQAAIPNHPDSLLPFSFYTVTGNTCRNIGDCKFPQE
                     CRPTDLAVDLERLISVIALDQELDASSLNIETDYSLLITPRIRAALGTPNSDLRWNWD
                     LSTHSFQLYDQSSPSRKIVIQISDVLPATFNFYNLDSIRGVSGLNGTYEHYFVMNGTD
                     TSGLEIVQFNGKIFLEEPSDSSQIFLGDCGRREPMACREMPYQTRTDLSKLMKDVLLN
                     QHPDSINLFSSIYMTDVLLSYYPDTNSHSTSNYIEYTSPGDTLFADTLRIVMDNCLVE
                     LYHDDDNDPSLPMDSLIGVGDLVAYGDPSDDGNYYSFLIPVTYSISNTTYTDTLYGTS
                     CFPLKNCVPCPQEQEEMEMMMMMVGGSSMNEEEINNALGQLFNLTENTPYTDSIAALF
                     GLVYDPTYANYSGYVHEINELNARNSWSSSDSLFVTPVSYEEFALRGYAAMNESYKRY
                     IHHYIHGLDSYEWLNNPVKFALGYGTGYNVNAEYQRYFAAVTRYNERAEAEGISDIST
                     MNVSTYRTEKYAERTAEYVDYIESYPQSGNPGDGIYEYYNSGFMMMAMSYPDSCDSLY
                     QIYLAAYTDFENYQALHPTCLDYQEIAPLYGYYNFEWNNLCCSDTGLALILDYIATLQ
                     DTMQCPGPLPYLATCDTVPDSTCFILYMQYLDDIDEYNESDYAAWADDSIINPYPTFE
                     SFVRSGLCSCQDQYHEYIAPYLNSPADTTLALPQSIDEFAPCLNSFALDKSPCGDNAY
                     RKYLNAVGKYNSWADANPGSGWPIITSILASNQFTPARYCYCVDAYVAFLKSIEEGMI
                     PYSSSLLYKLSLIEGCNNKITAECTPTQTITNIPPSPTVDPGNPCLDYLVDMALFNAQ
                     NAYNQYTDSLANAFRNKYRRHCLKALETFEAEYMDKEYHFTLYYYDQAGNLVKTVPPE
                     GVTPLNITSYQNTLAQRVNADRTNGTHTVFTGHLMATTYEYNSLNQLVKQYMPDHDPM
                     STWETSLPNGLDTRLQVTDIKFIDGNVGYLSGNITYPGSITRGWVYKTNDGGATWSKV
                     SGLAGTDLKKVFWVNDTLGYAVGSNGIMLKTINAGYIWDVLNGYRSHYALGELNDLYF
                     SSASAGIFVGANGVLLRTSDGGATFALVSYSGKLATDEIVSVTGNGTTVYMTLKRSSI
                     YHNHSFVLSSTNTGSTWTPANSNRTMDLRDVQYLNNGLVFAAGNNGHFIERIKPGATF
                     FWWQKTTGLELNFKQVYFMNENEGLAIVDSVSGFGQIFKTNDRGDNWELISEPGAYYN
                     AFFAYKTSGTTHKLVAVGRDRLVHRIIMMSGQPFGIIPMSNPPSTANNNFRACWAVEQ
                     ANGETWIFTGNDNGNVYFTDEGSESIQTWVSKGTLANGVRRIMFKNYVSGSSITLAGA
                     IINNTASSDAFPTFFSVTKVLTIGNYSPAPTASATFNDLEVDQANDRIYLQDETNRRI
                     YLAPISGSSITTTATLLNNQIPSNTPVAFSYNQADTIFAVHPTGAISRGVVNNSVSPP
                     TIAWTNQMDSTRMDLINDIQYSSAASTYFVCGERGYMAYLVSGVWRRMTTNIIDNLNA
                     IKLTSSTTGFLAADNGKIFSFIISGGWIVTCTEVPTTVSVNLNDILVNGNALMAVGDE
                     GTILFSNNITTTPLVRLNFNSTSDLHGVALRPGTVPAYTTVGENSTITLSFGGLVLQQ
                     KNVALPQIRSISFTDANNGFILADRFNIRQTSDGGVNWTPIKPGATINANYPVLNRIL
                     ATTAGKGIVVGNNRWYADISNNYTTQVTVGNFSASTATNLMDVATNGNVIFVAAQNST
                     NGQLWQKTGAGAWALATNAGPGISGTPLRKVHMFNNGNVLVVGTNGRIAYYNDVTNTI
                     TNWAAPTGLTTNTWLSIYFHDDLNGYVVGANGAVLKTSNNSLNGSGYHIFGSWVRMGT
                     DDNLNSQTDSTLMNIQAIGFANRNNGFIGGSYNGTPAGYARRVNDRDGRYSTLFWYDK
                     LGRIVLSQNTKQASTPTNERRYSYTRYDALGRVFEAGELTDTVTLASGTAFRDIFGDY
                     IANYYNPDVINDDSLTAFIGNNLRKEVTRSYYDSTWINSTPSGFAQNHLRKRIAHVTY
                     EETYDGIDTTYDHATHYSYDIHGNVKTLVQDNKKLADSTGIDDQQFKRIDYGYDLISG
                     NVHRVSYQKDSFDAFHHKYEYDADNRITTVYTSNHPSGDDITPLWDQDAKYFYYAHGP
                     LARVEIGDNQVQGVDYVYTLQGWIKGVNSSTLDSLRDPGCDGNRDSSNVNFNFAKDVF
                     GYSLNYFSGDYSPIASIHDTLKSFASISGSDIESNRYNLFNGNIGSMVTTITNPNTRE
                     VLPLGNAYKYDQLNRIIESKSFTNIDFATNQWQSGSSYAGMYYNGFSYDANGNILGQL
                     RKDHTGSVIDSLTYRYAKNSNNKIIQNRLYHVNDNTNYNASDINDMGTFTSSLASINS
                     ANNYGYSEIGELKFDKQEEISEIAWTVSGKVKYIYRPDTSSKKELGFDYDAMGQRIAK
                     HIYSSSGNWEKSTYYIRDAQGNVMSTYEHKVEEFMIGFKLNEQYIYGNSRLGMRTDTL
                     ELIGATLDTVNYERILCKKQYELSNHLGNVLTVVSDKKMPIESTLTSGTVGYFVADIT
                     HAQDFSPFGCVLENRNYELKPYRYSFQNQENDSEIKGSGNSINYTFRMHDTRLGRFFN
                     VDPLANSYAFLSAYQFSANRVIDAIELEGSELKYVRLTLATHANGDVYIKSVDEVRIW
                     KNVNFPVTVDGKTVDGTLTQIVYSYDGDDQTFLVERYEPKDYGDIFSVLKGLKSSANY
                     NYQSEEDMKQKAKDDATFMLNFLGKFSWWDLSKLQVFELLDARNNKAPEAVYENQGVT
                     DVLSAFPQMAAMKFIFIGSYKIRRDYFHKEVKPDILSAADRKKFEKIVGKNPDISVDK
                     NNKIVLVGTGDKYKGKTFETNLNADDFLKDE"
BASE COUNT         4227 a         2494 c         2746 g         3965 t
ORIGIN      
        1 gaattaaaaa agagactgag cgagggatta tgaccattca ggaatcgaaa tcaagcctaa
       61 ttaaaaactg atgagaacta catttttttc aagtctggtt ttatctctga tgatattcag
      121 tgtttcaccc gtattcgctt cctggaatgc agcaacgttg gaacaatatg aagaacattg
      181 caaaaaaatc agcgaaaact atgaaagact gaaggcttat tcagttctga ttcaacattc
      241 gtcctttcag ggacaaaatg aaaattctcc gttggccgac cagtcagagg gctttatcaa
      301 aaaggaagga attaaatttc atagttattt gagtggagta agatcagttc aaaacgaacg
      361 aatacgggtt tctgttgata catcacagaa aattctggtg attagtgagc cggaagacat
      421 tacaaaaaac gaagtggtat ccaataattg gaaagtaaca aggaaaaaca tcaaaggctg
      481 tttgcagaaa aagatccagg ataaaacagt gtacaggata gaatgcattt ccgggcctgt
      541 atttggtatt gaaatgattg taaatgcaac caatttaatt gatgaaatta cgatactcta
      601 tcgcagtgag tatttctctg aacaactgaa caagagtgtt cggccgatgc taaaaatcaa
      661 atattctcag tttatagaaa atccccgttt tgagaaaaat gatttttcta cagagaaata
      721 catcaccatt gataaaaaag gacagataat tcccacgcag gaatacttct cctataaatt
      781 aattgataca agatattcaa ccgcacaata acaggattaa catattccat ccgtaatgat
      841 gaaaaaaata agctactccg gactactaat tgcatttgtt gtattattta ctaatgtaaa
      901 tgcaggtcac cttgaattca gtaaccgaat ttttagcgtt cctgctgttt ccaatatttt
      961 ggtaaccgat caacttgaaa ttgaagtaaa tcctaaaaca ggatcattta ctgattataa
     1021 agtcagtaat attctgactc tcggtgttga tcatcattac agtgaatttt ttgccactgc
     1081 gatggaggta aaagtgaagt tttctctttc gcagtacaat tctgcaaact cactggtagg
     1141 tacaagcagc aaatacctca ctattaagta cgatcctttt ggaggtgctt accaggataa
     1201 atcttccttt ttattttcca atgcctatcg tttcgttgca gagatcgaca gtatttatgt
     1261 aaatggcgtt ccaaaagata gtcttcccgg tttattattt gtagaaggat ataccaatat
     1321 tgatcgtttt tataattttt catcagcagc aactacccca attgtactca atgcattaac
     1381 tacatctgag gtcgattgtg atggagctga tgatcatatc accgtttcct ggcccgcagt
     1441 aactggagca gaagaatatc atcttgagtg gtcctttgtt aatgattaca atgacagctt
     1501 aacatttaaa ccggcatctg cattaacatt agatttcaga aacaatgcaa cacgggtaag
     1561 tactaaagcg gaaaactata ctattccatt gttatttgag catggctatc tcgcattcag
     1621 agttcgtcct gtggggaggg atcactcatc acccgataat tatatttttg gcgtatggag
     1681 cttacctgaa aaggttaaag catcagatct ggatacttct gttgtgcatg ttatctttcc
     1741 acatgaagca aataaaaact ggcaagtcac aactacattc gcggaagaag gaaagaaaaa
     1801 agaggtaatc agttatttcg atggaacatt aagaaaccgc cagactgtaa ccagaattaa
     1861 ctcggataat catataatag tcgggcaaac aatttatgat catcagggaa gacctgcggt
     1921 gactgtattg ccggttcctg tgcaggactc ggtatgcgac aatagttttg ttgatcattc
     1981 gcttaagtat tatccaaatt ttaatttaga tgatacccta aacttgtatg ggagaaacga
     2041 tttcgatctg gatgatccgg actcggcatg cgcggcaaat gcggcaccaa tgtcggtgac
     2101 cagtggttcg tccaattatt attcgaatga taatccaaat cagaacggct accaatctta
     2161 tgttccgaat tcagaaaagt atccacttgt tcaggtcgaa tacacacctg ataatacagg
     2221 tcgaattcgt cgtcagggcg gagtgggacc tgaatttcag ttaggaagcg gtcatgaaac
     2281 attctactat tatggtcagc ctaaccagat tcaattggac cgattatttg gctccgaagc
     2341 aggcgatgcg gctcactaca agaagaatgt ggtaattgac cccaacgggc aggcctccgt
     2401 ttcttatctc gatcaggaag gcagggttat tgccacttcg cttgccggag atacaactca
     2461 gaatttgctt gcgctcgatt cggaaaatgg cgctgctgtg caattgacca tcgacctgtt
     2521 ctccaaaaat tcaaacggag tcagcaatac aaatgttgtt actcaagata gtacaggaat
     2581 taaattcagt actcaattat tggttgcgta ttcttccaat tttgaattca attatagcat
     2641 cgaagtggat actgtagatg atgcatgcct gaaagacaat atttgctttc attgtgtgta
     2701 tgatctggtc attagcattc acgatgaatg tatgcagctg gtatattcca ccgacactat
     2761 aataggtcat tttacaatgg aagacacatt aattgtattt gataccttgt gtacagatcc
     2821 tacagcatta aatttcgacc gtaatttcac tcttgctctt gagccgggaa attatacggt
     2881 atacaagcat cttcgcatca atgaagctgc tatggaacat tacctgaatc tttatcttga
     2941 tacaacgtat aattcctgct ttaaaacact ggcagaattt caggaagaat atttgtcaaa
     3001 tatcgactcg gcatcatgtc aaatcagttg tgagtcgtgt attgaagcat taggagaccg
     3061 tgatgaattt gttgcatccg gaaacggaac cgcatttgaa tgggacagac gttatgagga
     3121 atgcagatta atgtgtactg atactgaaga gccatgcgaa gcagaattga tgatgatgct
     3181 gggagatgtt tcaccaaacg gacaatacgg acagtaccta acaggaagcg gttctatttc
     3241 accaacatcg tttcctttgt ctgtctttaa tcaaacaaat aaattgccgg agagctggaa
     3301 tacaccagga caagcttatt ggcgtaaacc gaaaattgaa ttaaatgaaa ccgtatatcc
     3361 atattatgta gatgaagata atcaacgcag caaagtttac attgtaccta atgggaacaa
     3421 ttatattcct gctgtatcag atacttcttc ggcttatgtt tttacagata accagggagg
     3481 aaaatatacc tggcctgaaa atttatcaga tgttgcagat tttatcgatt tatggaaaac
     3541 atcctggtca agatccttgg tctatctgca tcctgaaatt tgctattacc agacttgttt
     3601 gagctatagt gaaaaagcga tcgatactaa tagtttcagc agccatagtt ttgatgtgtt
     3661 attgcgaaac acaaattcat tttatcaggc taaaaatgca ggactcataa aatcgaatta
     3721 cgcatcgttt cctgatccaa acgaccgtgt taccgattgg tttatcaatc aatcaggaac
     3781 taccattgca tgggatcctt ttgtagttta caatacagga tatggtccgg ctttggaaca
     3841 ggtttttgat acctatgtaa cttatggtgg caattcctat tcgatggtag aaattgctgc
     3901 tcttactgca cgatgcggag gcttgaccgg aattaatcct tccggtacat gccttttgtt
     3961 tggaagcgat atgtatcccg gtggaccggc tgcattaaac gatagtatcc ggaatttaga
     4021 gtggaataat ctcagaagtt tttatttaag taaaaagcta atcatgcagt cgaactatgc
     4081 cgataacatt gcgctgaatg attgtgaagc agtaaatgat tgcatcggta atcagaattt
     4141 taatgctgtt gaatcgggaa tgttttctgg aggtggattt accaacagtc cttggtttga
     4201 cggttctcag ccatgcagtg tgcataatta tcaatattat ctgaataaga tatcccgttt
     4261 tccggatcct tcggatatgg aacacatcgt tggttcctct gatgttcatg atgtcaatta
     4321 tcaacactat ttacaaaccg ggcaatgtcc tttagccgtg cacatgcagg aaatgctggg
     4381 agcactagct catgccgacg tattggattc aaccgatatt caaatgatgc agttccctgg
     4441 tttctcaacc tggtttcttg atttttccgg gtattcggtt aattccagta ttaatacttt
     4501 tgatttatac tggaatacga cttcagccgg aaatactatt tcaattactt taaccaatgg
     4561 ttctggtgga gctgctatct gtacaggcgc attagtaaaa gacacttcaa ttgcatcatg
     4621 ggatgatata tcattcttta ccggcattca atacacacac agtattagca accagcatta
     4681 ttttacaatg caggccgcaa ttcccaatca tcccgattca ttattgcctt tttcgtttta
     4741 tacagtaaca ggaaatactt gccgtaatat tggggattgt aagtttccgc aagaatgcag
     4801 acctacagat cttgctgttg atttagaacg gctcattagt gtaattgctc tcgatcagga
     4861 actcgacgct tcttcattga atatagaaac tgattattct ttattaatca ctccaagaat
     4921 aagagccgca ttaggtacac ccaacagcga tctccgctgg aattgggatc tgagtacaca
     4981 ttctttccaa ctgtatgatc aatccagtcc atcgcgtaaa atagtaatac agatttcaga
     5041 tgttttaccg gcaacattta atttctataa ccttgatagt attcgcggag taagtggact
     5101 taatgggact tatgagcact attttgtaat gaatgggaca gacaccagcg gacttgaaat
     5161 tgtgcaattc aatgggaaaa tttttctgga ggagccttcc gattcaagtc agatcttttt
     5221 gggtgattgt ggacgccgcg agccaatggc ctgccgtgaa atgccatatc aaacacgcac
     5281 cgatttaagc aagttgatga aagatgtttt gttgaatcaa caccctgata gcattaatct
     5341 gttttcaagt atttatatga ctgatgtttt gttgtcgtac tatccggata caaacagtca
     5401 ttctacctct aattacattg aatacacttc acccggggat acgctttttg ctgatacact
     5461 tagaattgta atggataatt gtcttgtgga attgtatcat gatgatgaca atgatccttc
     5521 tcttccaatg gatagcctta ttggtgttgg tgatcttgtg gcttatggtg atccatctga
     5581 cgatggaaat tactattcgt tccttattcc tgttacctat agtatcagca ataccaccta
     5641 tacagatacc ctttatggaa cctcttgttt cccgcttaaa aactgcgtgc cttgtccgca
     5701 ggagcaggaa gaaatggaaa tgatgatgat gatggtaggt ggctcaagta tgaatgaaga
     5761 agaaataaat aacgcccttg gccagctctt taatctaacc gaaaacactc catataccga
     5821 tagtattgct gctctttttg gtttggtgta tgacccaacc tatgcaaatt atagcgggta
     5881 tgttcatgaa attaatgaac tcaatgcaag gaacagttgg agttcatccg attctttatt
     5941 tgtaacacct gtttcttatg aagaatttgc actaagagga tacgcggcaa tgaacgaatc
     6001 ctacaaacgc tacattcacc attacattca tggtcttgat tcgtatgaat ggttgaataa
     6061 tccggtgaag tttgctttag gttatggaac aggatacaat gtgaatgcgg aatatcagcg
     6121 ttattttgca gcagttacac gatacaatga acgtgcagaa gcagaaggaa tatcagatat
     6181 ttctacaatg aatgtgagca cctaccgtac agaaaagtat gcggagcgca cagcagaata
     6241 tgtggattac attgaatctt atcctcaaag tggaaatcct ggcgatggaa tctatgagta
     6301 ttacaacagt gggtttatga tgatggccat gagttatcct gatagttgcg attctttata
     6361 ccagatttat ttagcagctt ataccgattt tgaaaattac caggcacttc atcctacatg
     6421 tttggattat caggaaatag caccgctata tggctattat aattttgagt ggaataactt
     6481 gtgttgttcg gataccggtc tcgcccttat acttgattac attgcaacat tgcaggacac
     6541 catgcaatgt cccggaccat taccttatct agcaacatgc gatacagttc ctgattcaac
     6601 ttgttttatc ttgtatatgc aatatcttga tgatattgat gagtacaatg aatctgatta
     6661 tgctgcctgg gccgatgatt ccatcattaa tccttatccc acattcgaat cctttgtgcg
     6721 gtccggattg tgttcctgcc aggatcagta ccatgaatac attgcacctt atttaaattc
     6781 gcctgccgat accacattgg cacttccgca atcgatagat gagtttgctc cttgtctgaa
     6841 ttcgtttgcc ttggataaaa gcccctgtgg cgataatgct taccgcaaat accttaatgc
     6901 agtaggtaaa tacaactctt gggcagatgc gaatccagga agcggttggc caataattac
     6961 aagtatattg gcatctaatc aatttactcc tgcaagatat tgttactgtg ttgacgccta
     7021 tgttgcattt ttaaaatcaa ttgaggaagg aatgatacct tattccagct ccttgcttta
     7081 taaactttct ctaattgaag gttgcaacaa caagatcaca gctgaatgta cgcctactca
     7141 aaccattacc aatattccac catcaccaac ggttgatccg gggaatcctt gtttggatta
     7201 tctggtggac atggcacttt ttaatgcgca aaacgcatac aaccagtata ccgatagtct
     7261 tgccaacgcc ttccggaata aataccgcag gcattgttta aaagcgcttg aaacctttga
     7321 ggcggagtat atggataaag agtatcactt tacactttat tattacgatc aggccggtaa
     7381 tttggtgaag accgttccac cggaaggtgt aacgccttta aatatcacct cttatcaaaa
     7441 caccctggcg caacgggtta acgcagatcg taccaacgga acccatactg tgtttacagg
     7501 tcatttaatg gctactacct acgaatacaa ttccttgaat cagctggtaa aacagtacat
     7561 gcccgaccat gatccaatga gtacctggga aacctcttta ccaaacgggc ttgacacacg
     7621 cttacaggtt accgatatca aatttattga tggtaatgtc ggttatttat cgggcaacat
     7681 tacttacccg ggaagtatta ccagaggatg ggtttacaaa acgaacgacg gtggtgcaac
     7741 atggtcgaaa gtctccggat tggcaggaac cgatctgaaa aaagtattct gggtaaatga
     7801 tacccttgga tacgcagtag gttccaatgg aatcatgctc aaaacaatta atgctggata
     7861 tatctgggac gttttaaatg gatataggag tcattatgca ttaggtgagc ttaatgatct
     7921 atatttctcc tctgcatctg caggaatatt tgttggtgca aatggagtac ttttaaggac
     7981 atccgatggg ggggcaacgt ttgctttagt ctcttattct ggtaaactag caactgacga
     8041 aattgtaagt gtcacaggta atggtactac tgtttatatg acattaaaga gatctagtat
     8101 ttatcataat cattcttttg tattaagtag tactaataca ggctcaactt ggactcctgc
     8161 taatagcaat agaactatgg atctccgtga cgtacaatac ttaaataacg ggttagtatt
     8221 cgcagcggga aataacggcc attttattga acgaattaaa cctggggcta cttttttctg
     8281 gtggcaaaag acaacaggcc tcgaattgaa ttttaagcaa gtatatttca tgaatgaaaa
     8341 tgaaggactt gcaattgtcg attcggtaag tggatttggt cagattttta aaacaaacga
     8401 tcgtggagat aactgggaac tgatcagtga accaggagct tattacaacg cattttttgc
     8461 ctataaaact tctggaacaa cccataaact agttgcagta ggacgagacc gtttagttca
     8521 tcgaattatt atgatgagtg gtcagccctt cggaattatc cctatgagca atccgccttc
     8581 tacagcaaat aacaatttcc gagcatgttg ggctgtagaa caggccaatg gcgaaacctg
     8641 gatttttaca gggaatgata atggaaacgt ttactttaca gatgaaggat ctgaatccat
     8701 tcaaacttgg gtcagcaaag gaacgctagc caacggtgtt cggagaatca tgtttaaaaa
     8761 ttatgtcagt ggttcttcca ttacattagc cggagctatt attaataata ctgcgtcttc
     8821 agatgctttt cctacatttt tcagtgtcac gaaagtgtta actattggga actatagccc
     8881 ggccccgact gcatctgcca catttaatga tcttgaagtg gatcaggcga acgacagaat
     8941 ttatctgcag gatgaaacca acagaagaat ttaccttgca cctatcagtg gatcttcgat
     9001 tacaacaaca gccacacttt taaataatca aattccatct aatacgccag tagctttttc
     9061 ctataatcaa gcagatacga tttttgcagt acatccaact ggtgcaattt ccaggggagt
     9121 ggtaaacaat tcggtttccc ctccaactat cgcatggacg aatcagatgg atagtacacg
     9181 aatggatttg ataaatgata ttcaatacag ctcagctgca tcaacttatt ttgtttgtgg
     9241 agaacgaggt tatatggcat acttggtaag tggagtatgg aggagaatga caacaaatat
     9301 tattgataat cttaatgcga taaaattaac ttcctctaca acaggattcc ttgctgcaga
     9361 taatggaaag atattctcat ttattatttc cgggggatgg attgtaacat gcaccgaagt
     9421 tccaacaaca gtatctgtta atctcaatga tattttagtt aatggtaatg ctctgatggc
     9481 agttggagat gaggggacga tattattttc aaataacatt accacaacac ctttggttcg
     9541 tctgaatttc aattcaactt cagacctcca tggtgttgca ttgcgacccg gtactgtgcc
     9601 tgcgtatacg acagtaggtg aaaatagtac gattacatta tcttttggag gattggtact
     9661 tcagcagaaa aatgtggcac tgccacaaat cagaagtatc agctttacag atgctaataa
     9721 tggttttata ttagctgata gatttaatat acgccagaca tccgatggtg gagtcaattg
     9781 gactcccatt aaaccaggag caacaattaa tgcaaattac cctgtattaa atcgaatact
     9841 tgcaacaaca gctgggaagg gtatcgttgt tggaaataat cgatggtatg ctgatatttc
     9901 aaataattat actacccaag ttacagtggg aaattttagt gcttctacag caacaaactt
     9961 gatggatgtt gcaacaaatg ggaatgtaat ttttgtagca gcccaaaatt ccacaaatgg
    10021 ccaactttgg caaaaaacag gtgctggtgc ttgggccttg gctactaatg caggtcctgg
    10081 aatttccggc actccgttac gaaaagtcca catgttcaac aatggaaatg tattggtagt
    10141 agggacaaat ggaagaattg catattataa tgatgttacc aatactatta ccaattgggc
    10201 agcccctacc ggcttaacaa ctaatacctg gctcagcatt tacttccacg acgacctcaa
    10261 tgggtatgta gtaggtgcaa atggtgcagt gcttaaaaca tcaaacaaca gcctaaacgg
    10321 cagcggctac cacatctttg gtagttgggt acgtatggga actgacgata acctgaattc
    10381 acaaaccgat agtactttaa tgaatattca agccatcggt tttgctaacc gcaataacgg
    10441 attcattggc ggaagctata acggaactcc cgcaggttat gcccgaagag ttaatgatcg
    10501 ggatgggcgt tattccacct tgttctggta cgataaacta gggcggattg tgctttctca
    10561 aaacaccaaa caggcctcaa cccctaccaa tgaacgcagg tattcgtaca cccgttacga
    10621 tgcgcttggg cgggtatttg aggcaggcga actcaccgat accgtaacac ttgcttccgg
    10681 aaccgcattc cgtgatattt tcggtgacta tattgctaat tattacaatc ctgacgtgat
    10741 caacgacgat tcgctgactg catttattgg aaataacctg cgcaaagaag tgacccgcag
    10801 ttattacgat tcaacctgga tcaattcgac gccttcaggt ttcgcgcaaa accacctgag
    10861 aaagcggatt gcacatgtaa cctacgaaga aacctacgat ggtattgata cgacctacga
    10921 tcacgctaca cactacagtt acgacattca tgggaatgtg aagacacttg tgcaggataa
    10981 caagaagttg gcggactcaa caggaattga tgatcagcaa tttaaacgga ttgattacgg
    11041 ttatgatctc atctcaggga acgttcacag agtaagttat cagaaagaca gctttgatgc
    11101 attccatcac aaatacgagt atgatgcgga taacaggata accactgtat atacttcgaa
    11161 tcatccatca ggtgatgata ttaccccgct ttgggatcag gacgcgaagt atttttatta
    11221 cgctcatggg ccactcgcta gagtagaaat tggagataat caagtgcagg gagtggatta
    11281 tgtgtatact ttgcaaggtt ggataaaagg agtcaactcc tctaccctcg actcgcttag
    11341 agaccccgga tgcgacggca accgcgacag cagcaatgtt aatttcaatt tcgcgaagga
    11401 tgtatttggt tacagtctga attatttttc cggggattac tctccaattg cttcaataca
    11461 tgatacttta aaatcgtttg caagtatttc aggaagcgac atagaaagta accgttataa
    11521 tttgtttaat ggaaatattg gatcaatggt gactaccata acgaatccaa atacaagaga
    11581 ggtattaccg cttgggaatg catataaata cgaccaactt aacagaatca tcgaatcaaa
    11641 atcatttacc aatattgatt ttgcaaccaa ccagtggcaa agtggcagca gctacgcagg
    11701 gatgtattat aatggatttt cgtatgatgc caatggaaat atattaggtc agttaagaaa
    11761 agaccacaca ggaagcgtga tagattcgct gacgtatcgc tatgctaaaa acagcaacaa
    11821 caagatcatc caaaatcgat tataccatgt aaacgataat accaattaca acgcaagtga
    11881 tattaatgat atgggaactt ttacctccag tttagccagt attaatagcg ctaataatta
    11941 cggctattcg gaaatcggcg agctgaaatt tgataaacag gaagaaattt cggaaattgc
    12001 atggactgta agtggaaagg taaaatatat ttatcgccca gacacttcat ccaaaaaaga
    12061 actaggtttt gattacgatg caatgggaca aagaatagct aaacatatat attcatcaag
    12121 tgggaattgg gaaaaatcca cgtattatat tagagatgct caaggcaatg taatgtccac
    12181 ttatgaacat aaagtagaag aatttatgat aggcttcaag ttaaatgagc aatacatcta
    12241 cggaaactcg agactaggaa tgcgaacaga tacgttagaa ttaatcggtg caacattgga
    12301 taccgtaaac tacgaaagaa tactttgtaa aaaacaatac gagttaagta atcacttagg
    12361 taatgtactc actgttgtgt cggataagaa gatgccaatt gagagtactt taacctcagg
    12421 aactgttgga tattttgtag cagatataac ccatgcacaa gatttttcac cttttggttg
    12481 cgttttagaa aatagaaatt acgaattaaa accatatagg tattcatttc aaaatcagga
    12541 aaatgattca gaaattaaag gcagtggaaa tagtataaat tatactttta ggatgcacga
    12601 tacgaggctt ggaaggtttt ttaatgttga tcctttagca aatagttacg catttttatc
    12661 tgcgtatcaa tttagtgcaa atagagtaat agacgctatt gaacttgaag gcagcgaatt
    12721 aaaatatgtt agattaactt tagctacaca tgctaatggt gatgtttaca ttaaaagtgt
    12781 ggatgaggtt cgcatttgga aaaacgttaa ttttccggtt actgtagacg gcaagacagt
    12841 ggatggtact ttgactcaaa tagtttacag ttatgatgga gacgatcaaa catttttagt
    12901 tgaacgttat gagcctaaag attatggaga tattttttct gtgctaaaag gattgaagtc
    12961 atccgcgaat tataattatc aaagtgagga ggacatgaaa caaaaagcta aagatgatgc
    13021 tacttttatg ttaaactttt tgggaaagtt tagctggtgg gatctcagta aattacaagt
    13081 atttgagtta ttagatgcta gaaataataa agctccagaa gccgtgtatg aaaatcaagg
    13141 agtgactgat gttttatcag catttcctca aatggcagca atgaaattta tctttattgg
    13201 ttcgtataaa attcgaagag attattttca caaagaagtt aaaccagata tattaagcgc
    13261 cgctgatagg aaaaaatttg aaaaaattgt cggaaaaaac cccgatatta gtgtagataa
    13321 aaacaataaa atagtactag ttggaacagg agataaatat aaagggaaaa cttttgagac
    13381 aaatttaaat gcagatgatt ttttaaaaga tgaataatga aaaaatacga ac
//
LOCUS       DAOMYG010000019        13272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1563_length_13272_cov_10.782326, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000019 DAOMYG010000000
VERSION     DAOMYG010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 13272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13272
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1563_length_13272_cov_10.782326"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1467)
                     /locus_tag="PK610_01315"
     CDS             complement(37..1467)
                     /locus_tag="PK610_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRJ34589.1"
                     /translation="MQRTMRRRNNLFVGILLLLFFTDLKANDSLKLSFNDYMGMVVRF
                     HPVARQATLLNDQSRYNLMMARGNFDPTLGADFSNKFYSGTQYYALLNTLATVPIWFG
                     PEIKMGFDQSQGAYVNAMDQMPASGLLYAGIKVPLAQGLLIDHRRAAVMQARIFERAN
                     EQQQQLILNELFYEASRCYYDWMLYDQNVLVYRNGVDLARQRLNFVRSLHAVGERPGV
                     DTLEAFIQLQSRQMLLNEAELVSKNQKLVLSVFLWGEDMRPMDLRDDVHPDRIDLSLI
                     KSVSPAEDQRGMDTLSTSHPAMLLYNYKLQDLEIERRYKTDKLKPKLSAQYNFLGYPV
                     SGNDFPVFSTENYKWGLQFSFPVFLRQERGAIGLTKVKIQQAQLDRDLKSMELQVKWR
                     SAYNELLFLREQMQMAESNLVNYRRLLDAERMKFVQGESSLFMINAREVMVIETEVKF
                     NEIRAKYFKSYAAYRFASGFANRMNP"
     gene            complement(1439..2800)
                     /locus_tag="PK610_01320"
     CDS             complement(1439..2800)
                     /locus_tag="PK610_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRJ34590.1"
                     /translation="MLNISPKSISGKIDPSKYNSFAAIGQYPLARRILLWLTIIFGVF
                     LLFLFLPWTQNILATGQITTLRPEQRPHTIHSTIPGRIEKWYVQEGQHVDRGDTIAFI
                     SEIKDDYFDPKLLQNTEDQLKAKEQSIDSYQSKVNALNKQIDALNETLILKLEQTRNK
                     IKQAELKLKSDSIDYEASITNYTIAEQQYNRYIELKEKDVISQTDLEGRKLKFQEAMA
                     KKISAENKVLASRNELLNSKIELNTIETEYNDKLMKAESDKYSAISTVFDTEAAVAKL
                     QSQFSGYAIRSGYYFITAPQDGYVTKALTTGIGETIKEGTPIVSIMPSKYELAVEVFV
                     EPMDLPLLVLGQPVRLVFDGWPSFVFSGWPGASFGTFGGKIVAVDNLMSDNGKYRVLV
                     SPDRNDEPWPVALRVGTGARGMMLLQDVPIWYELWRKLNGFPPEFYQPANPANAKNNE
                     KKK"
     gene            complement(2804..4501)
                     /locus_tag="PK610_01325"
     CDS             complement(2804..4501)
                     /locus_tag="PK610_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ34591.1"
                     /translation="MTPLRRFFLLVKPERRDIFQIYLYALFSGMIYLTLPLGIQAITS
                     FIMTGQLSTSWSVLVVMVILGIVLSGIIQVFQLSLTESLQQKIFVRAAFEFAYRVIRF
                     RKESLRGYYPPELMNRFFDVIQIQKGLSKILIDLSSSSLQIVFGLLLLSFYHPLFIAL
                     GFVLILVLYGIFKILSPRGLSTSLRESTHKYAVVQWLQELARVNDTFKLAGRTDLPYE
                     RMDTHVKQYVQSRKEHFRVLVWQYFTIILFKTVITGGLLILGSILVVNNQLNLGQFIA
                     AEILIVMVMQSAEKLVVSLESVYDVITAVEKIGMVTDLEMESLEGVDFKTAEDPEGIQ
                     IELRNLTYGYNAEEAAVLNDLNLTINAGEFVCISGVHHAGKSTLIQLMAGLFQDFHGN
                     VLYNGLPLRSIHLESLRCYISDSLSQEELFIGTVYENICMGKTNISERRVMEVAYAIG
                     LGDFLKDLPEGIQTRILPGGKKFPKSVVRKIILCRSLVDETRLLLLEDILLHVDPSER
                     DRLFALVFDKTRKRTVVVVSNDNRVAELCDRVVIMEKGRIIDQGPYSSLKKYNSIFG"
     gene            complement(4781..5785)
                     /locus_tag="PK610_01330"
     CDS             complement(4781..5785)
                     /locus_tag="PK610_01330"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009418696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRJ34592.1"
                     /translation="MRVAVVGATGLVGTKMMQVLQERNFPVTEFYAVASERSVGKEVV
                     FKGKTYKVIGMQEAVNLRPDIALFSAGGSTSLEWAPLFAEAGTTVIDNSSAWRMDPSK
                     KLIVPEINADALTADDKIIANPNCSTIQMVVALAPLHKKYGIKRVVVSTYQSVTGTGK
                     LAVDQLMNERKGIKGEMAYKYPIDLNVIPQIDVFLDNGYTKEEMKMVNETKKILRDES
                     VRVTATAVRIPVMGGHSESVNVEFAKDFDIDEARKMLAASAGIIVSDDLSTQHYPMPK
                     DAHEKDEVFVGRIRRDETQPNTLNMWIVSDNLRKGAATNAVQIAEYLVKSKVLTPLAV
                     "
     gene            5861..9244
                     /locus_tag="PK610_01335"
     CDS             5861..9244
                     /locus_tag="PK610_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRJ34593.1"
                     /translation="MKNLLKFYTLLLFLSPVLVQAQLIDNFTDGDFTNGITWSGNDAD
                     WTVVSGQLRSNGPAVTTTTIYLSTPSTAAMGAQWEFYVNPTCATSSGNYMIVTLMSDQ
                     ANITGSYNGYYVMIGNTADEISLYRKDGASATMIIDGANGSVASSSNNPFRIKVIRTL
                     AGAWTLERDNTGTGSNYFLEGTVTDNTYTSSSFFGVQATYSAANNQKYFFDDLYAGPI
                     IVDVSPPVISSITITGSNSIDVDFNENVELNSSQTVGNYLVNNGIGNPSTAIRDGSDL
                     SLVHLTFGSAFTPLLQNTITINNVEDLFSNAINNAQSNFTYVVFSTAGWRDVVINEIF
                     ADPSPSVGLPAFEFIEIHNRSTAVFNLAGWSFSDGSSSATLPSHTLLPGEFVILCPSG
                     ALASFSPFGTSIGLSNFPSLNNTGDNLFLLNGSGALIDQVNYSDTWYQNSVKAQGGWT
                     LELINPTLECSGSSNWIASNDNSGGTPGTQNSVYSNAPDITAPDAISVTIVNNGELII
                     GFNENMDSLSLVNATWTISGLSIADIEPIAPEFMNVRLLVSPAIDSTTAYTLIVSGAQ
                     DCSGNSMVNDTLQFAIGRQAQVFEVVIHEIYANPNGSSQLPEYEFVELRNVTNSVLRI
                     TGYKFSDATSTVTLPSIVLFPQEKIILVANANVAQFSSLGRTIGLSSLPSLNNSSDVL
                     TLKKADGTIIHTVAYFDTWYGDDTKKNGGWTLEMIDPLNPCNENNNWRASNDPSGGTP
                     GRENSVHAANPDLTAPVPSLVEVLSPTTIAVTFSERMDSLSMLTGNFTITNGINVIAQ
                     SVNGDRNVVLTVAPSLIPQTIYYLTAGLSTDCSGNTVASTPVKFAIPDFPQAGDLIIN
                     EVLFNPRGSGVDFVEIYNRSDRFITLKDWKIANGQNDTVSNIKTITTTNTLIYPGEYF
                     ALTTNPANIISEYPLSATDRLFTVASMPSFNNDAGRVFLITPLNSISDDFSYTDDMHF
                     PLLNSDDGVSLERVDFFRPTSDNTNWHSAAETVGFATPGYKNSQLQPGEVGNTLSVAP
                     KTFSPDNDGYNDVVNISYKFDQAGYTGNITIFDAQGRIVRLLMRNDLLSSEGTISWDG
                     INERREKANTGVYVVYMEVFDLSGNSKHYKTAVVLAMRF"
     gene            9247..9831
                     /locus_tag="PK610_01340"
     CDS             9247..9831
                     /locus_tag="PK610_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34594.1"
                     /translation="MKKLQQYHEQAGRKENAFEIWNAEPSDFQKTLRAFFLFCLLFSS
                     IIFIDYFLPRKTQQEVVTNWRAQSQYADLAKGDKRIEALHEIEPLDYRISTASTTIAL
                     QKNDADHISPGDTIIIRKTILFSTTTSLTIAGNKTIYPFMNFYSWLLFIPLCLTLFSL
                     LALFRPLKTESLSSMAVLNILLLAAFVTLRLFYL"
     gene            complement(9960..10724)
                     /locus_tag="PK610_01345"
     CDS             complement(9960..10724)
                     /locus_tag="PK610_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRJ34595.1"
                     /translation="MNTRISAIVVDDEKNSREVLIKLLAKRHPEIHIVGEAADVEEAY
                     ELCSNLKPQLVFLDIQMPKANGFNLLKKFDKIPFEVIFVTSYDQYAITAIKFNALDYL
                     LKPVELSELQQAILKVRNNIESKTNQQPQVINLIHSLESDLQDKKFAVHSGEKVKIIN
                     GNDIVVIKGDGRYCHLIMNSNETLIMTRNLKDFEDYFGVKSGFIRISKTYMINAAYIR
                     EYSKGDPFIITLTDNQSFEVSRRKKSEILTRLKVNG"
     gene            complement(10721..>13272)
                     /locus_tag="PK610_01350"
     CDS             complement(10721..>13272)
                     /locus_tag="PK610_01350"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRJ34596.1"
                     /translation="KRNGDLVINSQQRTFLISNETGNIVDLTKNKNVDPQTYIVLFKD
                     NAFDYFVKNNTGVLFDVNNNNRFHLIISKGPNSKELLVELDEETRMDWRTRICNINGN
                     TFLTIHNKLIQADEQLNVKITSFPSVITCIYANKDHGLWVGTSSNGVYHYNDITNLNE
                     YTHGLSGLTISSVLVDKEGGAWCATTEKGIYYSANYNVTYYPEVKALNKKTTFMKSDA
                     GNLFLSTEIDKLTYLEKNQFKSTEILNTGNGEITDICRFKNQYFIANKGYLGVLDKDF
                     KLKQVILKTFFPPGNLTTYQLDTSVTSLYALGVGLIYRINGSEIKPVGPSLPSKGRCM
                     KVMNDTIVYVGCNDGLYKINLLSIDVKKIGEVSTPVTKILLSNSGKIYFTTKGEGLFK
                     LIDEKAIAVPLDGDATVLNDIVEAKNNLLWISSNNGLIALSTDVEKTNSRRYNTSAGL
                     VSNNIGHLAIHNNYLYISSPDGVFRFPLETDLVNHTPSKVYLNEIRVNDSISISADYD
                     LEFEYWQNSITFSFDLINFKQGNKECLLYQLKGRDSDFQKNYSNVVVFENLSPNTYEL
                     VVNSMNSDGVIGSEPLRFKFRILPPFWQTWWFVFLLVIVSLLIIFMTVKMYIDTIRRS
                     EEEKTRVNKLISESQLSALQAQMNPHFIFNAINSIQNYILEKKEDDAYDYLVKFSKLV
                     RLVLNNSREKEISLHAELEMLQLYVSLEQLRFDKSFEFILQVSDDLNLFEIEIPTMLI
                     QPYVENAIWHGLMNLNGERQAVLKIDISQEDNILRIDIQDNGIGRARSNAFKRDSLHH
                     SVGMQLTEKRIEMINTIWNLKNVQVSITDLYDEQQESMGTKVELFLSVLTS"
BASE COUNT         3888 a         2933 c         2541 g         3910 t
ORIGIN      
        1 ttggtaatca tgttaaactg aatcatggtg tttgttttat ggattcatac gattggcaaa
       61 gcccgaagca aatcgataag cagcgtaact tttgaaatac ttagcccgga tctcattgaa
      121 tttcacttcg gtttctatta ccatcacttc gcgggcattg atcatgaaaa gactgctttc
      181 tccctgtaca aacttcattc gctctgcatc aagcaaacga cgataattca ccaggttgga
      241 ttcggccatc tgcatttgct cacgtaaaaa caacagctca ttataggccg atctccattt
      301 tacctgaagt tccatgctct ttagatcacg atcgagttgc gcttgttgaa tttttacttt
      361 ggtcaagcct attgcaccgc gttcctggcg aaggaataca gggaaggaaa attggagtcc
      421 ccacttataa ttctcggttg aaaaaaccgg aaaatcatta ccggatacag gataacctaa
      481 aaagttgtat tgggctgaaa gtttgggttt gagtttgtcg gttttatacc tgcgttcaat
      541 ttcaagatcc tgcaacttat aattgtataa caacatggca ggatgtgaag tgctcaaggt
      601 atccatgcct ctttgatctt ctgcagggga aacagatttt atcaatgaaa gatcaatgcg
      661 gtccggatga acatcatcac gcaaatccat cggacgcata tcttctcccc agagaaaaac
      721 ggataaaaca agtttctgat ttttagaaac caattcagct tcgttcagca acatttgacg
      781 gctttgtaat tggataaatg cttccagtgt atcaacaccg ggacgctccc caacagcatg
      841 tagcgaccgc acgaaattca accgctgtct cgccaggtca acaccgttac gatacaccag
      901 cacattctga tcatatagca tccaatcgta ataacacctg gatgcttcat agaaaagctc
      961 attgagaata agctgctgtt gctgttcatt tgcacgttcg aagatacgag cctgcatcac
     1021 ggctgctctc cggtgatcga tgagtaaacc ctgagcaagg ggcactttta ttcctgcata
     1081 aagcaaacca cttgcaggca tctggtccat cgcatttaca taagctccct gcgattgatc
     1141 gaatcccatt tttatttccg gaccaaacca aatgggaact gtagcaagtg tattcagtaa
     1201 agcgtaatac tgagtgccgg aataaaattt gttactgaaa tctgcaccaa gagtcggatc
     1261 aaaatttccg cgcgccatca tgaggttgta gcgcgactga tcgttgagta atgtggcctg
     1321 acgtgcaacg ggatgaaagc gtaccaccat ccccatgtaa tcattaaagc taagtttcaa
     1381 tgagtcgttc gccttcagat ccgtaaagaa aagaagcaga agaatgccaa cgaaaagatt
     1441 atttcttctt ctcattgttc tttgcatttg caggatttgc aggctgataa aactccggcg
     1501 gaaaaccatt cagcttccgc cataattcat accagatcgg tacatcctgt agcaacatca
     1561 ttccacgtgc acctgttccc acacgcaagg caaccggcca gggttcatca ttacgatcag
     1621 gactaaccag cacgcggtat tttccattat cgctcatgag attatctact gcaacaatct
     1681 ttccgccgaa cgtaccaaag gaagctcccg gccaaccgct gaacacaaag gaaggccaac
     1741 catcaaagac cagtcgaaca ggctgaccca atacaagcaa tggcaaatcc atcggttcca
     1801 caaaaacttc tacagcaagt tcatacttgg agggcatgat actaacaatg ggcgttccct
     1861 ccttaatggt ttcaccaata ccggtagtaa gtgctttggt gacatatcca tcttgtggtg
     1921 cggtgataaa ataataccca ctgcgaatgg cataacccga aaactgactc tgtaattttg
     1981 caacagctgc ttccgtatca aaaaccgtag agatggccga atacttatcc gactctgctt
     2041 tcatcagttt gtcgttgtat tcagtttcaa tagtattcaa ctcgattttg gaattcagca
     2101 attcatttcg cgaagccagg accttgtttt cggcagaaat cttttttgcc atggcctcct
     2161 gaaatttcaa tttacgtcct tccagatcgg tttgagaaat cacatctttc tctttcaatt
     2221 cgatgtaacg attgtattgc tgctctgcaa ttgtgtaatt ggtgatggaa gcctcatagt
     2281 cgatactgtc gcttttcagc ttcaactcgg cctgcttaat tttatttcgt gtttgttcca
     2341 gttttagaat cagcgtttca ttcagcgcat caatttgctt gttcaaggca ttcactttcg
     2401 attgatagga atcaatgctc tgttcttttg ccttcagttg atcttcggtg ttttgcaata
     2461 gtttcggatc gaaatagtcg tcttttattt ccgaaataaa tgcaatggta tcacctcggt
     2521 ctacatgctg accttcctgt acgtaccact tttcaatccg gccgggaata gtggaatgaa
     2581 tggtatgcgg acgttgttcg gggcgtaagg ttgtaatttg cccggttgcc agaatgtttt
     2641 gtgtccaagg caaaaacagg aagagcagaa acacaccgaa aatgatagta agccacaaca
     2701 aaatacggcg cgccaaggga tattgtccga tcgctgcgaa ggaattgtat ttcgaaggat
     2761 cgatcttgcc ggatatggat ttgggagaga tattcagcat gacttatcca aatatagaat
     2821 tgtacttctt caacgatgaa taaggcccct gatcaatgat cctgcccttt tccattatta
     2881 ctaccctgtc gcataattct gcaacacggt tatcattgga aacaacaaca acggtacgtt
     2941 ttctggtctt gtcaaaaaca agcgcaaata atctgtcgcg ttcggaggga tcaacatgca
     3001 gcagaatatc ttcaagcaat aataagcggg tttcatccac aagacttcga caaagtataa
     3061 tctttcgcac gacactcttt ggaaacttct ttccacccgg taaaattctg gtttgaattc
     3121 cttcaggcag atctttcaaa aaatcaccca aaccgattgc ataagcaact tccattacac
     3181 ggcgttcgct gatgtttgtt ttgcccatgc agatgttctc ataaaccgtt ccaatgaata
     3241 actcctcctg cgaaagagaa tcgctgatgt aacatcgcaa agattccaga tgaatgctcc
     3301 gcaatggcaa tccgttatac aaaacattgc catgaaaatc ctgaaacaaa ccggccatta
     3361 actgaatgag ggttgatttt ccggcatggt gtactccgga gatacaaaca aactctcctg
     3421 cgttaatggt tagattgagg tcgtttaaca cagcggcttc ttccgcattg tatccgtaag
     3481 taagattacg caattcaatt tggattcctt caggatcttc ggcggtttta aaatctactc
     3541 cttcgagcga ttccatttcc aaatcagtga ccataccaat tttctccacc gcagttatta
     3601 catcatatac cgactccagt gatacaacca gtttttctgc gctttgcatc accattacaa
     3661 taagaatttc agccgcaata aactgaccaa gattcaattg attgttcacc acaagaatac
     3721 tccccagaat caagagtcca cctgtaataa ccgttttaaa aaggataatg gtaaaatact
     3781 gccacaccag tactctgaag tgttccttgc gcgactgcac atattgcttc acgtgggtgt
     3841 ccattcgttc atacggaaga tcggtacgac ctgcgagctt gaaggtgtcg ttcactcggg
     3901 cgagttcctg cagccattgc actactgcgt acttgtgggt ggattctctc aatgaggtgg
     3961 ataaaccgcg cggacttaaa atcttaaaga ttccgtaaag cacaaggatg agtacaaaac
     4021 ctagcgcgat aaaaagagga tgataaaagg agagcaataa taatccgaat acaatctgca
     4081 gtgatgagga agaaagatcg atgaggattt tcgataatcc cttttggatc tgaatgacat
     4141 caaaaaagcg attcatgagt tcgggcgggt aatatccccg aagagactcc ttcctgaaac
     4201 gaataacccg ataagcgaat tcaaatgctg cccgtacaaa tatcttttgt tggaggcttt
     4261 cagtaagact cagctgaaac acctgaatga ttcccgaaag cacaattccc agaatgacca
     4321 tcacaaccaa aacagaccag gaagtactca gttggccggt catgataaaa gacgtgatgg
     4381 cctgaatacc caatggcagg gtaaggtaga tcataccgga aaacaaggcg tagagataga
     4441 tctgaaagat atcgcgtcgc tcaggcttaa caagaagaaa gaaccgtcgt agtggtgtca
     4501 tggtttggaa ttgctgcaaa actattcctg caggcgacac aataacaagg ttttgccaaa
     4561 ttttgtttat tgttttttac acaatttaac acaaaacgag tccagacacc ggtaaaatgc
     4621 caattccact gagggaacgg caaattccgg gccggtggtg aaagaatcac gcagtagtgt
     4681 acaattgaaa caattttgca ccctaaaagt tactcccaaa accggatgtg tgacaagcag
     4741 aagaagggat ctgaggaaag gcgggaaaac aaggatgaaa ttaaacagcc agtggagtta
     4801 ataccttgct tttcacgagg tattcagcaa tttgcaccgc attggtagct gcccctttgc
     4861 ggagattgtc cgaaacaatc cacatgttca aggtatttgg ctgggtttca tcgcgacgaa
     4921 tacgtccaac aaaaacctca tccttttcat gagcatcctt tggcattgga taatgctgtg
     4981 tggacagatc atccgaaacg ataatgccgg ctgaagcagc taacattttg cgagcctcat
     5041 cgatatcaaa atctttggca aactccacat tcacactctc actgtgtccg cccattacag
     5101 gaatacgcac agctgtagcg gtaacacgca cactctcgtc acgaagaatt ttcttggttt
     5161 cgttgaccat tttcatctcc tctttcgtat atccgttatc gaggaaaaca tcgatctgcg
     5221 gaatgacatt caggtcgatg gggtatttat atgccatttc gcctttgatg cctttgcgtt
     5281 cgttcatcaa ctgatctaca gcaagttttc cggttcctgt aacagattgg taagttgata
     5341 ccacaacgcg tttgatccca tactttttat gcaatggagc aagtgcaacc accatttgta
     5401 tggttgaaca attcggattg gcaataattt tatcatcggc cgtcaatgca tctgcattaa
     5461 tttccggaac gatgagcttc tttgaaggat ccattctcca ggcggaagaa ttatcgatta
     5521 cggtagtgcc tgcttcagca aaaagcggtg cccattcgag cgaagtgctt cctccagctg
     5581 aaaaaagtgc gatgtcggga cgcagattta ccgcctcctg cattccgatt accttatagg
     5641 tcttcccctt aaacaccact tcttttccaa cactgcgctc cgaagcaaca gcatagaatt
     5701 ccgttacagg gaaatttctt tcctgtaaca cctgcatcat ttttgtgcca accaaaccgg
     5761 tagctcctac tacagcaact cgcataattt cttaagaatt aaataacctt taatgagagg
     5821 gcaaatgtac tatatttaca catggacgat tttccatacg atgaagaatc tgttaaaatt
     5881 ttatacgctg ctgctgttcc tgtcgcctgt tcttgtacaa gcacagttaa tcgataattt
     5941 cactgatggt gattttacca atggcataac ctggagcggt aacgatgccg actggacagt
     6001 agtttccggt caattgcgtt ccaatggtcc tgcagttacc accaccacca tttacctcag
     6061 tactcccagt acagcagcaa tgggtgcgca atgggaattt tatgtgaatc ccacctgcgc
     6121 cacttccagt ggtaattata tgatcgtaac actgatgagt gatcaggcca acattaccgg
     6181 tagttacaat ggttattatg taatgatcgg aaatactgcc gacgaaatca gtctgtaccg
     6241 taaggatggg gcaagtgcaa caatgatcat tgacggtgca aacggaagtg tagcctcttc
     6301 gtccaataac cccttccgga taaaagtaat acgcacacta gccggagcgt ggacactcga
     6361 acgcgacaat accggaacag gaagcaatta ctttcttgag ggtacggtaa ccgataatac
     6421 ttatacaagc tcttcgttct ttggagtgca ggcaacatat tctgctgcca acaaccaaaa
     6481 atattttttt gatgatctgt atgcaggacc aattattgtt gatgttagtc cgccggtaat
     6541 ttcttccatt accattacag gaagcaatag tattgatgtc gactttaacg aaaacgtaga
     6601 gctgaactca tcgcaaaccg ttggcaatta tttagtcaat aacggaatcg gaaatccttc
     6661 aacagcaatt cgcgacggca gtgatctttc gctggtacat ttaacttttg gcagcgcctt
     6721 tacacctttg cttcagaaca ccattaccat caacaatgta gaagatcttt tcagcaatgc
     6781 gattaataat gcgcaatcca attttaccta tgtcgtattc agcactgcag gatggagaga
     6841 tgtggtgatc aacgaaattt ttgccgatcc ttcgccttcg gtgggcttgc ctgcatttga
     6901 atttattgaa attcataaca gaagtactgc tgtgtttaat ctggcggggt ggtcgttcag
     6961 tgatggaagt tcatctgcaa cattgccttc gcacactctt ttaccgggcg aatttgtaat
     7021 tctttgtcca agcggtgcgc tcgcttcctt ctctcctttc ggaacaagta tcggattaag
     7081 caattttccc tcactcaata ataccggcga caacctcttt cttctcaatg gcagcggtgc
     7141 gttaatcgat caggtaaatt attccgacac ctggtaccaa aacagtgtaa aggcacaggg
     7201 cgggtggaca cttgagctga tcaaccccac tctcgaatgc agcggaagca gcaactggat
     7261 cgcatcgaac gacaatagcg gaggaacacc gggaacacaa aatagtgtat atagtaatgc
     7321 gccggatatt acagcgcccg atgccatttc cgttacaatt gtaaacaacg gagaactgat
     7381 cattggtttc aatgaaaaca tggacagtct ttcgcttgta aatgccacct ggaccattag
     7441 cggattaagc attgcagaca ttgaacccat tgctcctgaa tttatgaatg tgcgtctgtt
     7501 ggttagtcct gccatcgact caactaccgc ctatacactt attgtttcag gtgctcagga
     7561 ttgctccgga aacagtatgg ttaatgacac cttgcaattt gcgattggac gtcaggcaca
     7621 ggtatttgaa gtggtgatcc atgagatcta tgcaaacccc aacggaagtt cacagttacc
     7681 tgaatacgag tttgtagaat tgagaaatgt caccaatagt gttttgcgca ttacaggata
     7741 caaattctcg gatgctacat ccacagtaac attaccctcc attgtgcttt tcccgcagga
     7801 aaaaataatt ctcgtagcca atgccaatgt tgcacaattc agttctttgg gcagaacgat
     7861 tggtttatca tcattgcctt cgctaaacaa ttcttccgat gttttaacac tgaaaaaagc
     7921 agacggaacc atcattcata ctgttgctta tttcgataca tggtacggcg acgataccaa
     7981 aaagaacggt ggctggacgc tcgaaatgat cgatcctttg aatccctgca acgagaacaa
     8041 taactggaga gcttccaatg atccaagtgg aggaacgccc ggaagagaaa attcagtgca
     8101 tgctgccaat cctgatctga ctgctcctgt gccttcactg gtggaagttc tttcgccaac
     8161 aacaattgct gttactttta gcgagcgaat ggattccctg agtatgctaa ccgggaattt
     8221 taccattaca aacggaatta acgttattgc acaaagtgta aacggagacc gcaatgtggt
     8281 attaaccgtt gcgccatcgc ttattcctca aaccatttat tacctcactg caggattaag
     8341 taccgattgc tcaggaaata cggtggcttc cactccggta aaatttgcca ttcctgattt
     8401 tccgcaggca ggggacctta tcattaatga agtgcttttt aatccaagag gaagtggcgt
     8461 tgattttgta gaaatctata accgttccga tcgcttcatc acactcaaag actggaaaat
     8521 tgcgaatggt cagaatgata cagtatcaaa cattaaaacg atcacaacaa caaacaccct
     8581 gatctatccc ggcgaatatt ttgcgcttac cactaatccg gccaacatca taagcgaata
     8641 tcctctatcc gcaacagatc gcttgtttac cgttgccagc atgccttcct ttaataacga
     8701 tgcaggaaga gtatttctca ttactccatt aaacagcatc agtgatgatt tctcctatac
     8761 cgatgatatg catttcccct tgttgaattc agacgatggt gtttcgctgg agcgggtaga
     8821 tttttttcgt ccgacatcgg ataacaccaa ttggcattca gcagcagaaa ccgtgggctt
     8881 tgcaacaccc ggatacaaaa attctcagct gcaacccgga gaagttggca atacattaag
     8941 cgtagctcca aaaacatttt ctccggataa cgacggctac aatgatgtgg tgaacatcag
     9001 ttacaaattc gatcaggcag gctataccgg aaacatcacc atattcgatg cgcagggtag
     9061 aatcgttcgc ttactgatgc gcaatgatct gttgtcttct gaaggcacca ttagctggga
     9121 cggcatcaac gaacgcaggg aaaaagccaa tacaggcgtt tatgtggtgt atatggaagt
     9181 gttcgacctg agtggaaaca gcaaacatta caaaacggct gtagtacttg ccatgcgatt
     9241 ctgagaatga aaaaactcca acagtaccat gaacaagcag gaagaaaaga aaatgcattc
     9301 gaaatctgga atgctgaacc ttccgatttt caaaaaacac taagagcttt ttttcttttt
     9361 tgtctgttgt tctcgtccat catcttcatc gattattttc taccgcgcaa aacacaacag
     9421 gaagtcgtta ccaactggag agcacaatcg caatacgccg atctcgcaaa gggcgataaa
     9481 cgcattgaag cattacacga aattgaaccg ctcgactacc gcatcagcac cgcatcgaca
     9541 acgatcgcac ttcagaaaaa cgatgccgac cacatttctc cgggagatac gattatcatc
     9601 agaaaaacaa tactgttcag cacaactacc tcattaacga tcgcaggaaa caaaacgatc
     9661 tatcccttta tgaattttta ttcatggctt ttattcattc ccctttgcct gacattattt
     9721 tctctgctgg cattgtttcg tccgctaaaa acagaatcgc tcagcagcat ggctgtttta
     9781 aatattcttc tcctggcggc gtttgtaacc ttgcggttat tttatttgtg aaggggtggt
     9841 gttgatgtga tctccttcaa aatcatttca gtgtttccac aaaatgtact ttaacattta
     9901 aggtaagcaa tagtgttatt ttatttactg tactattttt tggggttacc tttaacatcc
     9961 taaccattga cttttaaccg cgttagtatt tcagacttct ttctgcgaga aacttcaaat
    10021 gattgattgt cggtaagcgt tatgataaaa ggatctcctt tactgtattc tctgatgtat
    10081 gcagcattga tcatgtaggt tttacttatt cgaataaacc cggattttac cccaaaatag
    10141 tcttcaaaat ccttaagatt tcttgtcata atcaaggttt cattgctatt cataattaga
    10201 tgacaataac gcccgtcgcc ctttatcact acaatatcat tcccattaat aatttttacc
    10261 ttttctccac tgtgcacggc aaatttttta tcctgcaaat cagactccaa actatgtatt
    10321 aaattgatta cctgaggctg ttgattagtt tttgattcaa tattgtttct tactttcaat
    10381 atagcttgct gcagttcgct caattcaacc ggtttgagta aataatccag cgcattgaac
    10441 ttgattgcgg taatggcata ttgatcatag cttgtaacaa atattacttc gaagggaatc
    10501 ttatcaaatt tcttcaatag attaaaacca tttgctttgg gcatttgaat atccagaaaa
    10561 accaattggg gttttagatt gctacataat tcatatgcct cttcaacatc tgcggcttcc
    10621 cctacaatat gaatctcagg atgcctttta gcaagaagtt taataagcac ttctctactg
    10681 ttcttttcgt catcaacaac aattgcagaa attctcgtat tcatgaagtt aaaacagata
    10741 aaaacaattc aacttttgtt cccattgact cctgttgttc atcgtacaga tctgtaatac
    10801 ttacctgtac atttttcaaa ttccaaatgg tatttatcat ttcaattctc ttttcagtta
    10861 gttgcattcc aaccgagtga tgtaaagaat ctcttttaaa cgcgttcgac cgtgccctgc
    10921 caataccatt atcctgaata tctattctca atatattgtc ttcctgagaa atatcaattt
    10981 tcaaaacagc ttgtcgctca ccattcaaat tcataaggcc atgccagata gcattctcaa
    11041 catagggttg aatcaacatt gtaggtattt caatttcaaa taaattaaga tcatctgaaa
    11101 cttgtaagat aaattcgaaa cttttgtcaa atcgtaattg ttccagtgaa acatacaatt
    11161 gcagcatttc caattctgcg tgtaacgaaa tctccttttc tcttgaatta ttaagaacca
    11221 atcttacaag cttgctaaac ttaaccaaat agtcatatgc atcgtcctct ttcttttcca
    11281 ggatatagtt ttgaatgctg ttaatggcgt taaagataaa gtggggattc atttgtgcct
    11341 gaagtgcgct caactgcgat tccgaaatca atttattcac cctcgttttt tcctcctcac
    11401 ttcttcttat agtatcaata tacattttca ctgtcataaa aatgattagc aaagacacaa
    11461 taaccaataa aaaaacaaac caccaggttt gccaaaaagg gggcaaaatt ctgaacttaa
    11521 accggagcgg ttctgaccca ataactccat cgctgttcat gctgtttact accaattcat
    11581 aggtattggg tgacaggttc tcaaatacaa caacattgga gtaatttttt tgaaaatcgc
    11641 tatccctgcc tttaagctga taaagaagac actctttatt cccttgtttg aaattaatca
    11701 gatcaaaaga aaaagttatt gaattctgcc aatactcaaa ctccaaatca tagtcggcag
    11761 aaattgaaat cgaatcattt actctaattt catttaaata aacctttgat ggggtatgat
    11821 ttaccaaatc cgtttcaagt gggaatctaa aaactccatc aggagaggag atatataaat
    11881 agttattatg tatcgccaga tgcccgatat tgttcgaaac aagcccagcg gaagtgttat
    11941 atcttctgga gttcgttttt tcgacgtctg ttgaaagtgc tatcaatcca ttgttggatg
    12001 aaatccataa tagattattc ttagcttcaa caatatcatt taaaactgtt gcatcaccgt
    12061 ccaaaggcac tgctatagct ttttcgtcta tcagcttgaa caagccttca cccttggttg
    12121 taaaatatat ttttccgctg ttgctaagta aaatttttgt gaccggagtg ctgacctcgc
    12181 caattttttt gacatcaata gacagtaaat tgattttata caatccatca ttgcaaccca
    12241 cataaacaat agtatcattc attactttca tacatcttcc ttttgaagga agagatgggc
    12301 caaccggttt gatttctgat ccgtttatcc tatagatcaa cccaacgcct aatgcataca
    12361 aactcgtaac gcttgtatcg agttgatagg tagttaaatt tcccggaggg aaaaatgttt
    12421 tcaaaattac ctgcttcagt ttaaaatcct tatccaaaac tcccagatag cctttgtttg
    12481 caataaaata ttgattttta aacctgcaaa tatctgtgat ctcaccattt cctgtattga
    12541 gaatttctgt ggatttaaac tggttttttt ccaggtacgt cagtttatcg atttcagtag
    12601 agagaaataa atttcctgca tctgatttca taaatgtggt ttttttattc aaggctttta
    12661 cctcaggata atatgttacg ttgtagttgg ccgagtaata aatgcctttt tcagtagtag
    12721 cgcaccaagc ccccccctct ttatcaacta acacagaact aatggttaac ccggaaagac
    12781 catgtgtgta ctcattcaaa ttagtgatat cgttataatg gtacacccca tttgagctgg
    12841 taccgaccca caaaccatga tccttattcg cataaataca agtaataacg cttgggaaac
    12901 ttgtaatttt tacatttaat tgctcatctg cctgtatgag tttgttgtga atagttaaga
    12961 aagtattccc gttaatgttg caaattctgg ttctccaatc cattctggtt tcttcgtcta
    13021 attcaaccaa aagttctttg ctattagggc ctttacttat aattaaatga aacctgttat
    13081 tattattgac atcaaataag acaccggtat tgttttttac aaaataatca aatgcgttgt
    13141 ctttgaatag tacaatatat gtttgcgggt caacattttt gtttttagtc aaatcaacaa
    13201 tgtttcctgt ttcatttgat ataagaaatg ttcgttgctg agaattaata accaaatcac
    13261 cattgcgttt tc
//
LOCUS       DAOMYG010000020        13123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1585_length_13123_cov_9.502908, whole genome shotgun sequence.
ACCESSION   DAOMYG010000020 DAOMYG010000000
VERSION     DAOMYG010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 13123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13123
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1585_length_13123_cov_9.502908"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PK610_01355"
     CDS             complement(<1..804)
                     /locus_tag="PK610_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34597.1"
                     /translation="MRLLILLFSVLTLTKSYSQAFLPTSFSFNAASPPTGWILNLDVV
                     AGATTYATGSDGNPSCRLDGTGEYVLIEFSDEPDSLVYSLRGTATSPPASAPGTQFTI
                     QQSANGTTWSTVRLFDTSNMTTGFTSYTDILLSSTRYVRFYYTNKVSGSNVALDAITI
                     KKADPGPEPAIAAFYNSVQIQNGGTIIISNSGNNPIQLFNQGTSQTLTLGTATLSGAA
                     ASDYGLLNFPSSISSLGNSSFDLTFTPSQSGTRRHAGTTDRCRRSRQGRG"
     gene            complement(801..1844)
                     /locus_tag="PK610_01360"
     CDS             complement(801..1844)
                     /locus_tag="PK610_01360"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRJ34598.1"
                     /translation="MKNTLLLTALFICGFSTYGNSQTEKKFYSACIAFWNVENLYDTI
                     NEPVKNDEEFLPTGLNRWNTEKYLKKLDRLSEVIAQVGTEFNPDGPAIVGLAEIENIE
                     VLHDLVKQANIRSRNYKPILIEGPDRRGVDVALLYNPKYFSPKSQVLYTLSDPNDTSF
                     ATRGQLLVSGTLDGERMHFVVAHWPSRRGGEKRSRPKRALAANLGRHIIDSIQKAESG
                     AKVIYMGDLNDDPVNESVFKVIQASAKKTDSTGNKLYNPMYPLFKNGIGSLAWNDSWN
                     LFDQMLLTTTFLATQQPGFQFHLAKVFNKPFLRQDSGNFKGYPWRTFSAGTYSAGYAD
                     HFPVYLILKKEVK"
     gene            2035..4575
                     /locus_tag="PK610_01365"
     CDS             2035..4575
                     /locus_tag="PK610_01365"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ34599.1"
                     /translation="MTNLNFRGSYILILFLLLSVDGISQNDTSFRVKPDTLLEQGNRL
                     PIFTSTSGDNDDQTESQDISGLLQASRDVYASVAGFNFGNARFRIRGLNSDHTSVFIN
                     GIRVNDLETGWASWSNWGGLNDVTRWMEVKTGNASSRIGFTGIGGYSNIDARASSYRK
                     GNRVSYAFSNRSYAHRIMATGSTGMIENGLAITASVSRRYSDEGYVAGTFFDSWSYFL
                     SVEKRINAKHTLSFIGFGAPLRQGRQGLAVQEAMDLAGSNYYNPFWGYQNGEKRNSRV
                     SHNHEPMLMLTDHILISEKTKIQAGVFFSPGKSGQTSLNWYDAKDPRPDYYKYLPSYY
                     DPEYPALVPQVTNSWINDVNTRQINWDQLYFANSKNLYTVTNAEGVAGNNITGNRAKY
                     IVEDQRNDVTMGGLNLLGTHQYNERIRITGGLFHVRQKSHYYKLVDDLLGADFWLDLN
                     RFAVENAIDQSESQADINNPNNVVYKGEVFGYDYNLLINKTNFFAQGEYSGKKVDAYF
                     SAELSQTNFWREGNMLNGMFPNNSEGESEKFNFLNYGVKGGAVYKITGRHYVVANIAH
                     LTRAPLARNVFLSPRTRNQTASDVRSEKVYSGDIGYEIRYPRFKARITGYYNYVADQT
                     WMRTFFHEEYNSFVNYTLTGVDQSMAGLEFGAQYQVTPTLQLNGVVGHGQFLWANRPT
                     ATVTSDNQEKLIASEKTVYAKNFHIGGMPETAASLGIKYSGVKNWFAGVNFNYFANIW
                     LEINPDRRTEEAVEGLVITDPQWGELIDQSKLDNNYTIDAFAGKSWRIKSYFLNVNLN
                     VNNILNNRDFRIGGSEQLRYDTRDVNRFPNKYSYHLGTTFYAMVSLRF"
     gene            4594..5805
                     /locus_tag="PK610_01370"
     CDS             4594..5805
                     /locus_tag="PK610_01370"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HRJ34600.1"
                     /translation="MKQIFILLLAVAAFTSCKKEFDAPPSREIPTGSILTIADLRAMY
                     AGSPIHFQSDQTVFAVVTADEVSGNLYKNVYIQDNTGAINIRLRSSGGLYVGDSIRLN
                     LTGTVLSSYNGMMQIDSVDADNNIVKQATGRFIQPEVVTLNMINASYQAKLVRINNVQ
                     FVSSDTSLTFANAVAQTSQNRTIADCNGNTMLVRTSGYANFAGSNIPNGNGSIVVIVG
                     QFNSDMQLYIRTLSDLTMDGGTRCVEPYLEKDFDDLSISSGGWTQQLVTGGVSWSASS
                     FGPDIFGRISNYNGSNNVACESWLISPAVNLSSAINPVLTFRTACNYSGSNIQVYVLT
                     NYDGISNPSTATQTLLSPALSGGSWAWVNSGTLNLSSFLTSGVYIGFKYTGTNSDGKT
                     WEVDDILIEEL"
     gene            5817..7160
                     /locus_tag="PK610_01375"
     CDS             5817..7160
                     /locus_tag="PK610_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ34601.1"
                     /translation="MKRILPLVIAIVFAGTLSAQTIISTSFENWTGINPDGWKGTKTN
                     LQADSIIRVTTGTQYGSNAVQLNNPTTSHRRFSSQPVPVTAGQNFTVKYWAKGQGDIR
                     VGLFDSSNVVTTSGYHYSAYISVNTNTWTEYSQNITADTTWNTAQFIFSIRNTNATGG
                     HLKIDSAVVFIPAPTGPTSIYDIQYTTDAAGESPLIGQQVQTGGIVSAVYASGYFIQD
                     GVGPWRGLHVFDSNNFPAIGDSVRITGYVSEYFSMTQISSVSSFLVFSSSNPVYAPSV
                     ISTAAVNTEAYEGVLVRVNNATCVNANAGFGMWTVNDGSQECKIHNLIYAYTPTQNTI
                     YQITGPVYYAFAEFRIEPRDVADVVYQGTVGVEDGEMESEIQFYPNPVDNILNIKSSK
                     SISVVTILDLTGRIVLTTALDTNSSHINLELLIPGFYTLLVDQQGDSKSFVFIKK"
     gene            7373..10684
                     /locus_tag="PK610_01380"
     CDS             7373..10684
                     /locus_tag="PK610_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ34602.1"
                     /translation="MKHILLLALFIFFNCALSQGQSTTFRPDPSKIEQVIQNGFSFDL
                     VKSRLSANLNSDAVALMAHPGFEARIAKWHFTTKRILIKRYFEGSLSLLMFQSEIDQL
                     ISALIERINSDPASILTELNSFSAENNNSTVHSHAAHHTHNHSSVVEEGLHNESGEDT
                     TAVQQQMRIPGGPCVNPDFESCNFNDWTLVTGQVNGSAPYSFVNPTTTTTFGTTNGMP
                     ATSGSEQHYITTGGTDTYGFPMVYPGGSCSAEIGDFTGYNYMAAQIRKTFLVSAGDAI
                     LTLHYAVVLEDGGHTANEQPYFRMRVYDQAGNSISCAAYEAYANDGQPGWQSSGIWRY
                     KPWTTVFIPLAPYLGQNVTIEFTVGDCAQGGHAGYAYVDATCDAMAFNMSANAVCAGQ
                     PITINGPPGGASYLWSTGATTQSITTSTPGAYSVTVIPVTGSSCSITLDTVVNASPNP
                     IANFTHNSPVCFGTAVQFTDQSNPNGATINSWSWNFGNGATSTLQNPTHTYAAAGTYN
                     VQLTVSTTAGCTHSVTIPVTQAPDLTLPTSFVDENCGQCDGSAVVAPQGGTAPYSYNW
                     GAAGGNVPALNSLCAGNYPVTVTDNMGCTETATVVVGSGGSLSITNVATTPATCPSTC
                     NGTITVTAPGATQFSIDGGVTFQASNVFNNMCPGNYTVTASNSTGCNDTEPAVVTVPN
                     PLTLTPGPGSTICIGQSATVSASASGGTAPYTFTWNQGLPSAQSHNVTPLISTDYQVS
                     VSDNNGCNAGPLSITITVNPPLQVVVSPDVMICPGASATLTANASGGDGDPYNYSWTD
                     GAGFSQTGSSITVSPTTNTTYIVTATDNCGTPQTTAQVNVGLNPVPQVTFVADNLSGC
                     VPVAVNFTNTTDPALSGNCQWNFGNGQTSNNCNPSMVFNTPGCYDISLTVTSPAGCIG
                     SLTIPGMICAHPYPVADFVFGPQPTDLLNTTITFINTSAGASTYSWDFAGLGSSTAPN
                     TVFTFPDEDPGNYDVCLAVENAFGCQDTVCKVVVINDFFTIYVPNAFTPDNDGKNDIF
                     FPVIDGFVDGTYQIYVFNRWGEIVFESNDPNGYWDGKYRGVNAKQDVYVWKIVIKDAA
                     SKKKRTYYGHVSLLR"
     gene            11122..12810
                     /locus_tag="PK610_01385"
     CDS             11122..12810
                     /locus_tag="PK610_01385"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ34603.1"
                     /translation="MKKLLLALFTLFVFAFGAKAQDVGITSVISPVSGCCVGMDTVRL
                     YLYNYSAFPIGGNFTITYTVNGGAPISETILTSIGPSASYLFGFNQQWNFACQGTYNV
                     CCNVQFPGDVNPANNNFCVTIVSDTFVVAGTVSGPPVVCASANSGVMVLSGNNKNDSL
                     LWQMSVDGTNWINTMNDSSSYQFSNIGQTMYYQVFVDGGYCPDGTSSVHTIFVDSPPV
                     GGSITSNMNVCSGNNSAQLNLIGSAGDTIYWAASTGGGPYVPFVNDTTIFVFNNITQT
                     TQYQAYVANGVCPFVPSGIVTVLVDPPTIPGTVVGGATVCQGVNSGSMGLISSSGNIN
                     GWIYSSNNGATWQTSGNTTSSFNYLNISSSTIYCAIVQSTGCPADTSVCDTVFITPLP
                     IANAGVDDTIIIFDQTQLFGTGGVFYAWTPAGSLSDPTIADPIANPTTTTTYTLTVSD
                     AFGCFGTDQVTIFVIDTTTPPMPNTIIIANYMSLNGDGLNDVWNIMNIEYFPENEVTV
                     FNNQGMVVYEKSTYDNSWDGKFNGDQLPDGSYFYVVNLTKLGQKTKGVLTITSK"
     gene            12807..>13123
                     /locus_tag="PK610_01390"
     CDS             12807..>13123
                     /locus_tag="PK610_01390"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRJ34604.1"
                     /translation="MTSTSILYRFLLVIFLTPLAASAQQIPLYNHYHVNPFVYNPSLS
                     GYSGKTNAYFIRNQKYLGFEGGNVTNILTVDGPIFANKVGIGFSVFNDQMGPSIAQGA
                     GLS"
BASE COUNT         3804 a         2570 c         2829 g         3920 t
ORIGIN      
        1 tcctcgacct tggcgagagc ggcggcatcg atcagtggtc cctgcgtgac gcctggtgcc
       61 cgattgcgat ggcgtaaacg taagatcaaa agaggaattt cccaatgagg aaatggacga
      121 tgggaagttg agcagtccat aatcagaggc agcagcgcca gataatgttg ctgtacctaa
      181 tgttaatgtt tgcgatgtgc cctgattaaa tagttgaatg gggttattgc cggaattact
      241 gataataatt gtccctccgt tttggatttg tacactgtta tagaaagcag caattgcagg
      301 ttcgggaccg ggatcggctt tttttattgt aatggcatcc agagcaacat tcgatcctga
      361 aactttattg gtatagtaga aacgaacata tcgggtagaa gaaagcaaaa tatcggtgta
      421 ggatgtaaaa ccggttgtca tgttactggt gtcaaataat cggacagttg accatgtggt
      481 tccatttgca gattgttgaa tagtgaattg cgtgccaggt gcagatgcag gtggacttgt
      541 tgcagttcct cttaaactgt atactaacga atccggctca tcggaaaact caataagaac
      601 gtattctcct gtgccatcaa gcctgcaaga tggattccca tcacttcctg ttgcgtaggt
      661 agtggcacct gcaacaacat caagattgag gatccaaccg gttgggggac ttgctgcgtt
      721 gaaggaaaag cttgttggca aaaatgcctg tgaatagctt tttgtcaatg tcaaaacaga
      781 aaacaataaa atcaataatc tcatttcact tcttttttga ggatgagata aaccggaaag
      841 tgatctgcat atcctgctga gtatgttcct gcagaaaaag tccgccaggg gtagccttta
      901 aagttgccgc tgtcttgacg aagaaaaggt ttgttaaaga cttttgccag atgaaactgg
      961 aaaccgggtt gttgtgttgc aagaaaagtt gtagtgagta gcatctggtc aaaaaggttc
     1021 catgaatcat tccaggcaag tgatccgatg ccatttttaa atagcgggta catcggattg
     1081 taaagtttgt ttccggttga atcagttttt tttgcagagg cctgaattac tttaaaaaca
     1141 ctttcattaa caggatcgtc atttaggtct cccatataaa ttactttggc tccggactct
     1201 gctttttgaa ttgaatcaat aatatgtctc cctaaatttg cggcaagtgc acgttttggc
     1261 ctgcttcttt tttcaccccc tctgcgtgat ggccagtgcg caacaacaaa atgcattcgc
     1321 tctccatcca atgttccgct aaccaatagc tgtccccttg ttgcaaatga ggtgtcattt
     1381 ggatctgata atgtgtaaag cacctgcgat ttcggtgaga agtattttgg attgtacaac
     1441 aatgctacat ccacaccacg ccgatcagga ccttcgatga gaatcggctt ataattccga
     1501 cttctgatgt tggcttgttt aactaaatca tggagcacct caatgttttc aatctcagct
     1561 aatcctacta ttgccggacc atcgggatta aattctgtac ctacctgagc aataacttca
     1621 cttaaacgat ctagtttttt caaatacttt tcagtattcc accgattgag acctgttggc
     1681 agaaattctt catcgttttt tacaggctca ttaatagtgt cgtaaaggtt ctctacattc
     1741 caaaacgcaa tacatgcaga gtaaaatttc ttttcagttt gcgaatttcc gtatgtagaa
     1801 aaaccacaaa tgaacagggc agtaagcagc aaggtatttt tcatagacaa gtagtgaagc
     1861 aaaggtaggt aaatgagtcc ttcggatggt tatttcttga tttcttaaca aaagacatgc
     1921 aaatcaaaca tcaattcacc gatgttgatt attaaaatgt tactttttat tgttttaaca
     1981 acaagagaaa gataaaactt ccgacttttg tcgccgcaaa tgctacttgc ccgaatgacc
     2041 aacctaaact ttagaggatc ttatatcctt attctatttc ttctcttatc tgttgatggt
     2101 ataagccaga atgacacttc gtttcgtgtg aagccggata ccctgcttga acaagggaat
     2161 cgcctgccaa tttttaccag caccagcggc gataacgatg accaaacaga atcgcaggat
     2221 atctccggtt tattgcaagc ctcaagggat gtttatgctt ctgtagccgg atttaatttc
     2281 ggaaatgccc gcttccgtat tcgtggactg aattcggatc atacttcggt atttattaac
     2341 ggaattcggg tcaatgacct tgaaaccgga tgggcatcct ggagtaattg gggaggatta
     2401 aatgacgtta cccgatggat ggaagttaaa accggaaatg cttcctcgcg aattggattc
     2461 actggtatag gaggatattc caacattgat gcacgtgcaa gctcctaccg aaaaggcaat
     2521 cgtgtttctt atgcattttc taaccgaagc tatgcacatc gaattatggc aaccggttca
     2581 actggaatga tcgaaaatgg acttgcaatc acagcgtccg tttcgcggag atattcagac
     2641 gaaggttatg ttgcaggaac ctttttcgat tcctggagtt attttttatc agttgagaaa
     2701 agaattaatg caaaacatac gctatctttt atcggctttg gagcgccttt acgacagggt
     2761 agacaaggac tcgccgttca ggaagcaatg gatcttgcag gaagcaatta ttataatcca
     2821 ttttgggggt atcagaatgg agaaaaaaga aacagcaggg taagccataa tcatgagcca
     2881 atgctgatgc taacggacca cattttgatt tctgaaaaaa cgaaaattca ggccggagtt
     2941 ttcttttcac ctggtaaatc aggacaaacc agtttgaatt ggtatgacgc aaaagacccg
     3001 cgtcctgatt attataagta tttaccatct tattatgatc ctgaatatcc tgctcttgtt
     3061 cctcaggtaa caaactcatg gatcaatgat gtcaatacac ggcagataaa ttgggatcag
     3121 ctttatttcg caaatagcaa gaacctatat acggttacaa atgctgaagg tgttgccgga
     3181 aataacatta cgggtaatcg tgcaaaatat attgtagaag atcagcgcaa tgatgtaacg
     3241 atgggcggac taaatttact cggaacgcac cagtataacg aacgcatccg tattacaggt
     3301 ggtttgtttc atgttcgaca gaaaagtcat tattataagt tagttgacga tttgctgggc
     3361 gctgattttt ggctcgattt aaatcgtttt gctgttgaga atgcaattga tcaatccgag
     3421 tcgcaggctg acattaacaa ccctaacaat gtagtctata aaggagaggt ttttggttac
     3481 gattacaatt tgcttatcaa taaaacgaac ttttttgctc agggagaata ttccggtaag
     3541 aaggtggatg cttatttttc tgccgagcta agtcaaacaa atttctggcg ggaagggaat
     3601 atgttaaacg ggatgtttcc aaataatagc gaaggagagt cagagaaatt taattttctg
     3661 aattatggag tcaaaggcgg ggctgtgtat aagattaccg gtcgtcatta tgtagttgcg
     3721 aatattgcac acctcacaag agctccattg gcacgaaatg tcttcctttc tccgcgcact
     3781 cgtaaccaaa cagcatcaga tgttcgcagt gagaaagttt attccggcga tattggttat
     3841 gaaataaggt acccaaggtt taaagcacgt attacaggtt attacaatta tgttgcggat
     3901 cagacatgga tgcgtacgtt tttccatgaa gaatataatt cgtttgtgaa ttacacttta
     3961 accggggttg atcaatccat ggccggactc gagtttggcg cacaatatca ggttacacca
     4021 acattgcaat taaatggtgt tgttggtcat ggtcaatttt tgtgggcaaa ccgacctaca
     4081 gcaaccgtta cttccgataa tcaggaaaaa ttgattgcaa gtgaaaaaac agtttacgca
     4141 aagaattttc atattggcgg aatgccggaa acagcggcat cactcggaat taaatattcc
     4201 ggcgtgaaaa attggtttgc aggggtaaac tttaattatt ttgccaatat atggctggaa
     4261 attaatcctg accgacgtac agaagaagca gtggaaggtt tagttattac agacccgcaa
     4321 tggggtgaac tcattgatca gtcaaaactg gataacaatt atactattga tgcatttgct
     4381 ggtaaatcat ggagaataaa atcttatttt ttaaatgtaa atctcaacgt aaataacatt
     4441 ttaaataacc gcgatttccg tattggtgga tcagagcagt tacgttatga taccagagat
     4501 gtaaaccgtt ttccaaataa atattcttac catttgggaa caacatttta cgcaatggtt
     4561 agcttaagat tttaaaaaca gaatacgatc attatgaaac aaatatttat tttactactg
     4621 gctgttgcag catttacttc ctgcaaaaag gaattcgatg ctcctccatc aagagaaatc
     4681 cctacaggga gcattttaac cattgccgat ttgcgtgcaa tgtatgcagg aagtcctatc
     4741 cacttccaaa gcgatcaaac ggtatttgca gtagtaaccg cagatgaagt ttccggtaat
     4801 ttgtacaaga atgtttacat tcaggacaat acgggagcca ttaacatcag gttgcgcagc
     4861 tccggtggat tgtatgttgg ggattcaatt cgtcttaacc taaccggaac ggttttgtcg
     4921 tcttacaatg ggatgatgca gattgattct gtagatgccg ataataatat tgttaagcaa
     4981 gctaccggac gttttattca gcccgaagtt gtaacactta acatgataaa tgcatcttac
     5041 caggctaaat tggttcgaat caataatgtt cagtttgttt cttcagatac ttcattaact
     5101 tttgctaacg ctgtagccca aacttctcag aacagaacaa ttgcagattg caatggtaat
     5161 actatgcttg ttcgcaccag cgggtacgcc aactttgcgg gaagcaatat tccaaatgga
     5221 aatggttcaa tcgttgtaat tgtaggacag tttaattccg acatgcaatt gtacattcga
     5281 acattgtcgg atttgactat ggatgggggt actcgttgtg ttgagcctta tcttgaaaaa
     5341 gattttgatg acttaagcat aagctccggt ggatggaccc aacaattggt gaccggagga
     5401 gtttcatggt ctgcaagttc atttggtccg gatatttttg ggcggatttc gaactataac
     5461 ggaagcaata atgttgcttg tgaaagttgg ttgatttctc ccgcagtaaa tttgagcagt
     5521 gcaattaatc ctgtacttac cttccgaact gcatgtaatt attccggttc aaatattcaa
     5581 gtctatgtcc ttacgaatta cgacggtata agcaatccct caactgctac gcagacttta
     5641 ctgagtcctg cattatcagg aggatcatgg gcctgggtta attcaggaac attgaactta
     5701 tcatcatttc ttacaagcgg tgtttatatt ggatttaaat acacaggaac aaactccgat
     5761 ggtaaaacct gggaggttga cgatatttta attgaagagt tataaaaaga aagatcatga
     5821 aaagaatttt acccttagtt attgcaattg tttttgcagg cacattaagt gctcaaacaa
     5881 ttatcagcac aagttttgaa aactggacag gtatcaatcc tgacggttgg aaaggaacaa
     5941 aaacgaattt gcaggcggat agcattatcc gagttactac cggaactcaa tacggaagta
     6001 atgccgttca gctgaacaat ccaactacat cgcaccgtcg cttttcttcc cagcctgttc
     6061 cggttacagc cggtcagaat tttacagtaa aatactgggc aaaaggtcaa ggcgatattc
     6121 gcgttgggtt attcgatagt tccaatgttg ttacaacttc cggataccac tatagtgctt
     6181 acatttccgt gaacactaat acatggacag aatattctca aaatatcaca gcagatacca
     6241 catggaatac tgctcagttt attttctcta ttcgtaacac taacgcaaca ggaggtcatt
     6301 tgaaaattga ttcggccgtt gtgtttatcc cggctccaac aggtcctaca tcaatttacg
     6361 acatccaata cacaactgat gcggctggtg agtctccatt aatcggtcag caagtgcaaa
     6421 ccggtggtat tgtaagtgct gtttatgctt caggttattt tattcaggat ggtgttggtc
     6481 cttggagagg tttacatgtt ttcgattcaa acaacttccc ggcaattggc gactctgtta
     6541 ggattaccgg atatgtttca gaatacttca gcatgacaca aatttcaagt gtttcatcct
     6601 ttttggtgtt cagcagcagt aatcctgttt atgcaccttc cgtaatttca acagcagcag
     6661 tcaatacaga agcctatgaa ggtgttttgg tgcgggtaaa taatgctaca tgcgtaaatg
     6721 caaatgcagg ttttgggatg tggacagtta acgatggatc gcaggaatgc aaaattcaca
     6781 atttgattta tgcctataca ccaacgcaga ataccattta ccagattact ggccctgttt
     6841 attacgcatt tgccgagttt aggattgagc caagagatgt tgccgatgta gtttaccagg
     6901 gaaccgttgg agtggaggac ggtgaaatgg aatcagaaat ccaattttac ccaaaccctg
     6961 tagataatat attgaatatc aaatcatcaa agagtatttc agttgtgaca attctcgatt
     7021 tgacaggtcg aattgttttg accaccgcat tagacacgaa ttcttcacac ataaaccttg
     7081 aattgttaat ccccggcttc tacacacttt tagtagatca acaaggtgat tctaaatcgt
     7141 ttgtgtttat taagaagtaa gttgttctca aaataaaatt caaaaggagg ggcaaccctc
     7201 ctttttttgt tcgtcaaatc ttcggaattt gaagcggaaa ttaaggattg gaaggacgaa
     7261 acacagaaaa tataggttaa acgatttagt cgtttctgat tttctgaaac cttccaaact
     7321 aaaatatcag catcaaagac tataaattga cacaccaaaa atggttaatg ccatgaaaca
     7381 tattctattg cttgccttgt ttatcttttt taactgtgcg ttaagccagg ggcagtccac
     7441 gacttttcgt cctgatcctt ccaagatcga acaggttatt cagaatggat tcagttttga
     7501 cttggtaaaa tctcgtttaa gtgccaattt gaattcagat gcagttgccc tcatggctca
     7561 tcccggtttt gaggcgcgaa ttgcgaaatg gcattttact acaaagcgga ttttgatcaa
     7621 acgctatttt gaaggttcct tatcgctatt aatgttccaa agcgaaatcg accaacttat
     7681 atcagcccta attgaaagga tcaattctga cccggcgagc attttgaccg aattaaattc
     7741 attttctgct gagaataata attctacagt tcacagccat gctgcacatc atacccataa
     7801 tcattcatct gtagttgagg aaggcttgca caacgaatca ggcgaggata ctacagcagt
     7861 acaacaacaa atgagaatac ccggtggtcc ttgtgtgaat cctgatttcg agtcatgtaa
     7921 ttttaatgat tggacattag taaccggaca ggtaaacgga tctgcaccgt attcatttgt
     7981 taacccaacc accacaacaa cttttggaac gaccaacgga atgcctgcaa cgagcggaag
     8041 tgaacagcat tatatcacaa ccggtggaac tgacacctat ggttttccaa tggtttatcc
     8101 aggaggttct tgttctgcgg agattggaga ttttacgggg tataattata tggcagctca
     8161 aataagaaaa acattcctgg tctctgctgg tgatgcaatt cttacactgc attacgctgt
     8221 tgttttggaa gatggtgggc acacggcaaa tgaacaaccc tatttcagaa tgagggttta
     8281 cgaccaggct ggaaattcga tttcatgtgc tgcttacgag gcttacgcaa acgacggaca
     8341 accaggttgg caatcatccg gtatttggag atacaaaccc tggacaactg tttttattcc
     8401 tctggcgccg tatttaggtc agaatgtaac tattgaattc actgtcggag attgcgctca
     8461 gggtggtcat gcaggttatg cctatgtcga tgcaacttgt gacgcaatgg cgtttaatat
     8521 gtctgcgaat gctgtatgtg caggacaacc aattaccatt aacggacctc ctggcggggc
     8581 ttcctatctt tggagtaccg gtgctactac ccaatccatt actacttcca ctccgggtgc
     8641 ttattcagtt actgttattc cggtaaccgg ttcttcatgt tcaatcaccc tcgatacggt
     8701 tgtgaatgca agtccaaatc caattgccaa tttcactcat aattcgccgg tatgttttgg
     8761 tactgcggtt cagtttacag atcaatccaa tccaaatggt gcaacaataa acagttggag
     8821 ttggaatttt ggaaacggag caacttctac ccttcagaac ccaacgcata cttatgccgc
     8881 agcaggcacc tataacgtgc aattaactgt gtccacaaca gcaggatgta cgcattctgt
     8941 aactatacca gttacacaag cgccggattt aactttgcca acttcttttg tggatgaaaa
     9001 ctgtggacaa tgcgacggtt ctgcagttgt tgcaccacaa ggaggaactg caccgtattc
     9061 ctataactgg ggagctgcag gcggcaatgt ccctgcttta aatagtcttt gtgcaggcaa
     9121 ctatcctgta actgttactg ataatatggg atgtacagaa acagcaactg tggtagttgg
     9181 atcaggagga agtttatcca ttaccaacgt tgcaaccact cctgccactt gtccttcaac
     9241 ctgtaatggg actattacag ttactgcacc cggagcaaca cagttctcaa ttgatggcgg
     9301 tgttactttc caggcttcaa atgttttcaa taatatgtgc ccaggaaact atactgttac
     9361 tgcatcaaac agcacaggct gtaatgatac tgaacctgca gttgtaactg tccccaatcc
     9421 tctaacacta acacccgggc ctggatcaac catttgtatc ggtcagagtg caacggtatc
     9481 cgcttccgca agtggaggaa ccgctcccta cactttcaca tggaatcaag gcttgccaag
     9541 tgcgcaatca cataatgtaa caccgcttat ttctacagat tatcaagtca gcgtttcaga
     9601 taacaatggc tgcaatgccg gtcctttgag tattacgatt acggttaacc ctcctctaca
     9661 ggtagttgtt tcgccggatg tgatgatttg ccccggagca agcgcaacgc ttacagcaaa
     9721 tgcttcaggt ggggatggtg atccgtataa ttattcatgg actgatggcg caggatttag
     9781 ccagactgga agttcgatta ctgtttctcc tacaacaaac actacctaca ttgtaactgc
     9841 aacagataat tgcggtactc cacaaactac agcccaggta aacgtaggac tgaatcccgt
     9901 tccacaggta acttttgttg cagataattt atcgggttgc gtacctgttg ctgtgaattt
     9961 tacaaacaca acagatcctg cattatccgg gaattgccaa tggaatttcg gaaatggaca
    10021 aacatctaac aattgcaatc cttcaatggt atttaatacc ccgggatgtt atgatattag
    10081 tcttactgta acatcacctg cgggttgtat tggctcactc acaattccgg gaatgatctg
    10141 cgcacatccg tatccagttg cagattttgt atttggtccg cagcccaccg atttattaaa
    10201 cacaaccatt acattcatca atacctctgc aggtgcaagt acatatagtt gggactttgc
    10261 cggtttaggc agctcaactg cacctaatac tgtatttact ttcccggatg aagaccccgg
    10321 aaattatgat gtttgcctcg cagtagaaaa tgcatttggt tgccaggata cagtttgtaa
    10381 agttgttgtg attaacgact tctttacgat ctacgttcct aatgcgttta ctccagataa
    10441 tgatggaaaa aatgatattt tcttccctgt tatcgatggc tttgttgatg gtacttacca
    10501 gatttatgta tttaatcgct ggggagaaat tgtatttgaa tccaacgatc caaatggata
    10561 ttgggatgga aaatacagag gtgtcaatgc aaaacaggat gtttacgttt ggaaaattgt
    10621 aatcaaggat gctgcttcaa agaagaagcg tacttattat ggccatgttt cattattgag
    10681 ataagaagca cttgacctac taaccttttt tggcaaatct aaaggaccaa cacttgcttt
    10741 ttaactattg catttgtttc tctgttggct tagattgaat gttcaattga cttctgtaaa
    10801 ttgagataac gaagtgacgg ttttcaataa tcagtggatg gttgtttatg aaaagtagac
    10861 ctaggacaat tcataggatg gtaaattcaa tggcgaaaaa ctaccggacg gaaactactt
    10921 ttatgttgtt agtttaccga aactaggaca aaggacaaag ggtatttcga atatggccag
    10981 caataagcag acagtttaag catctgtata acagcttctt agtgtaaaga ttgaaatttt
    11041 tgaagtctaa attttaacct aattggttga tttctcgtag aattccttat ttttaatatc
    11101 cgattctaaa cggttttgtc aatgaaaaaa ctgttacttg ccttatttac acttttcgtc
    11161 tttgcatttg gtgctaaagc acaagacgtt ggaataactt ctgttatttc cccggttagc
    11221 ggttgctgtg ttggaatgga cacagtgcga ctttacttgt ataattatag tgctttccct
    11281 ataggaggta attttacaat tacttatacc gttaacggag gcgctccaat ttctgagacg
    11341 attcttacca gtattggtcc aagcgcgagc tatctttttg ggtttaatca gcaatggaat
    11401 tttgcctgtc agggcacgta taacgtgtgt tgcaacgttc agtttccggg tgatgtaaac
    11461 cctgcgaaca ataatttttg tgttactatt gtgagcgaca catttgttgt ggcgggcaca
    11521 gtatcaggtc ctccggtggt atgtgcatca gcgaactccg gtgtgatggt gttatcgggg
    11581 aataacaaga atgattcttt actatggcag atgtcggtag atggaacgaa ctggatcaac
    11641 acgatgaacg attcttcgag ttaccagttt agcaatattg gtcagaccat gtattatcag
    11701 gtgtttgttg acggaggtta ttgccctgat ggaacatctt ctgtacatac catatttgtg
    11761 gattcaccac ctgttggcgg aagtattacc agcaatatga acgtatgttc cggcaataat
    11821 tcagcacagt tgaatttaat tggttcagca ggggatacga tttactgggc agcatctaca
    11881 ggtggcggac cttatgttcc gtttgtgaac gacacgacga tatttgtttt taataatatc
    11941 acccaaacaa cgcaatatca ggcctacgta gccaatggag tatgtccatt tgttccaagc
    12001 ggaatagtaa cggtattggt agatccccca acaataccgg gaacggtagt tggaggtgca
    12061 acggtatgtc agggagtgaa ctccgggtct atgggtttga tatcgagttc cggaaacatc
    12121 aacggctgga tctattccag caataatggt gcgacgtggc aaacgtccgg gaataccact
    12181 tcgagtttta attatctgaa tatatcatcg agcacgattt attgtgcgat agtgcaaagt
    12241 acaggatgtc cggcagacac gtccgtttgt gatacggtat ttattactcc attaccgatt
    12301 gccaatgcag gtgtggatga tacgatcatc atatttgatc agacccaatt gtttggtaca
    12361 ggtggagtgt tttatgcatg gactcctgcc ggaagtttgt cggaccctac aatagccgat
    12421 cctatcgcaa atccaacaac taccacaacg tataccttaa cggtatcaga tgcatttgga
    12481 tgttttggta cagaccaggt caccattttt gtgatagata caacaacacc accaatgccg
    12541 aatacgatca ttattgcgaa ttatatgtcg ctaaatggag acgggttgaa tgatgtttgg
    12601 aacataatga atattgaata tttcccggag aacgaagtga cggttttcaa taatcagggg
    12661 atggttgttt atgaaaagtc gacttatgac aactcatggg acggtaaatt caacggagat
    12721 caattgccgg atggaagtta tttctatgtc gttaatttaa ccaagctggg acagaaaact
    12781 aagggagtgc taacgataac cagtaaatga catctacatc cattttatat cgatttttac
    12841 tggtaatatt tttgaccccg cttgcagcga gtgcgcaaca gattccctta tacaatcatt
    12901 accacgttaa tccttttgta tataatccgt cactttcagg atattcggga aaaacaaacg
    12961 cgtattttat tcgcaatcaa aaatacctgg gtttcgaagg tggaaatgtt acaaatattt
    13021 tgacagtcga cgggcctatt tttgccaata aggtggggat aggtttttca gtgtttaatg
    13081 atcaaatggg accaagtatc gctcaaggtg caggcttgtc ttt
//
LOCUS       DAOMYG010000021        13120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1587_length_13120_cov_9.306008, whole genome shotgun sequence.
ACCESSION   DAOMYG010000021 DAOMYG010000000
VERSION     DAOMYG010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 13120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13120
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1587_length_13120_cov_9.306008"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1855)
                     /locus_tag="PK610_01395"
     CDS             complement(110..1855)
                     /locus_tag="PK610_01395"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRJ34605.1"
                     /translation="MNPLSGFLLLAMSLAISPNTIAQTYPQNYFRSPMDIPLYLSGSF
                     GELRANHFHTGLDIKTQGVEGQRIYATAEGYISRIRVSHYGYGLVLYMNHPNGYTTVY
                     AHLSRFHSSIEKYIREKQYELRKEEVDLDSLPSELFVFKKGDVIAWSGNSGSSGGPHL
                     HFEVRETATEKALNPMLFGFDVKDDIKPTIHDIKIYPLTDTSVIDGQSSERMIKATGA
                     NGKYSLVKEVKARGTVGFAVHTIDMTTGSGNLCGVYEVELYMKDSLLWKQQMQCIDFR
                     TNRYINTHMDYLEHRDNKNSYHKSYIKGNNALDIYPVKVNNGWVTVQPGQRIKMKYLV
                     KDIKGNVSELQFTIVSDSTKIPPAKSDPCPKSSMEEYGLFPVHWDHPNIIRFMDMEVL
                     IPATGIYDNHCILVERHTRRKDAIAYTFRVGNSDIPVQEYMEISLPCDTLMEHLRPYA
                     VGIYINAKGKISSEGGIYSNGHVTFRTRSFGRYSVMLDTIAPVITAVNISEGKNVSAQ
                     KELQFTVSDNLSGVKTYEAFIDGEWKLLRYKVKTGKLILNMEEEKVTTGTHSLEVRVR
                     DERGNEALFKCTFTR"
     gene            2102..2416
                     /locus_tag="PK610_01400"
     CDS             2102..2416
                     /locus_tag="PK610_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34606.1"
                     /translation="MTASLKAKVERLIHHCDEVKRSEGQLQERVKVLEAERVQLQQFI
                     SQLHGEIDRRQSEIATLNRNMEVLKNAEALNQSDERSADMKRKINELMREIDNCITLL
                     SK"
     gene            2422..2709
                     /locus_tag="PK610_01405"
     CDS             2422..2709
                     /locus_tag="PK610_01405"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRJ34607.1"
                     /translation="MSEISIKVNIGTRSYPLSVQPGEEELIRAAAHDVNENIRSLQEN
                     YAVKDIQDLLAMTALTMATQLRQKSTGGMSDADAASLADIERMLDAYLSRV"
     gene            2787..4328
                     /gene="rny"
                     /locus_tag="PK610_01410"
     CDS             2787..4328
                     /gene="rny"
                     /locus_tag="PK610_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRJ34608.1"
                     /translation="MEPISLMIGAAAGLVLGGIIIYVILNGVLRKKREEVIREAEKEG
                     ENIKKDKILQAKEKFLQLKEEHEKAVIERDRKIQSAEDRIKSKEKELSRKMEDVTRTE
                     REAQSQRDTLNAQSEKLQKRQEELDKAHQKQVAELEKISGYSKEDAKAQLVEALKAEA
                     RTDAMAHVKEIMDEAKLTANKEAKRIVIQTIQRVATEQAVENAVSVFNIENDELKGRI
                     IGREGRNIRALEAATGVEIVVDDTPEAIILSCFDPVRREIARLALHQLVSDGRIHPAR
                     IEEIVSKTKHKLEEEIVEIGKRTTIDLGIHGLHPELIRMVGRMKYRSSYGQNLLQHSR
                     EVANLCAIMASELGLNPKIAKRAGLLHDIGKVPDEEPELPHAILGMKLAEKFGEKADI
                     CNAIGAHHDEIEMTTLYAPIVQVCDAISGARPGARREIVESYIKRIKELEELAQSYTG
                     VSQAYAIQAGRELRVMCEAEKVSDADVDKLAFDISQRIQTEMTYPGQVKITVIREKRA
                     VNYAK"
     gene            4449..5426
                     /locus_tag="PK610_01415"
     CDS             4449..5426
                     /locus_tag="PK610_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ34609.1"
                     /translation="MPSHPQHILITGAAGFIGSNLCEHFLELGYSVTGMDNFLTGKPS
                     NIAGFYSHPNFCFVEGDIRDKSKCGEAMKGISIVLHQAALGSVPRSVKDPLTTNSVNV
                     DGFLNVIHAAHQAGVKRFVYATSSSVYGDHSGLPKVEDAIGKPLSPYAASKYINELYA
                     GVFHRLHGMEVIGLRYFNVFGKHQDPDGAYAAAIPKFIKLFLEGKSPVIYGDGSNSRD
                     FPYIKNVIQANEKAAFTTNEKAFGEVFNIAFGERTTLLELITEIKNALSVHAPNVKTI
                     SVTHENERPGEIPHSLASIEKARNVLGYTPSHSLKAGLQEAVTWYLENI"
     gene            complement(5497..7704)
                     /locus_tag="PK610_01420"
     CDS             complement(5497..7704)
                     /locus_tag="PK610_01420"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HRJ34610.1"
                     /translation="MTRTGLLTVLLFFCNSLLHAQELHSVSAKKSEQIIKIDGLLEDD
                     AWKNAMIISDFRQWFPFDSSMAKSKTEVMVAFDKDHLHIAAICYDELPGEHVIQSLKR
                     DYDPLLSDGFYVYIDPFSDLTNGFVFGVNPHGAQMEGLIQYGGNMGISTVWDNKWFSE
                     VKQYHSEGKWIVEMAIPYKTLRFPENIKEWRINFARYDFKRNEISVWSGVPRNFSTSS
                     LAFTGKLLLEEAPHKEGSNVSLIPYAIGTATGNYQNQTAEKFRPNAGLDAKIAVNSSL
                     NLDLTINPDFAQVEVDRQVTNLSRFSLFFPERRNFFLENSDLFERFGFRQIRPFFSRR
                     IGLNNGEAVPILFGARLSGKINRDWRIGAMTMQTEGGSQSGLEGQNYSVAAVTYQMKG
                     RSNISGILVNRQGFIKQDMNFADYNRIAGIDWNIATMDNRLMGKIFMHHSFSPHKTEN
                     AMAHASWIHYETPKLVLEWNHEYVGHNYSADVGFVPRIDFFNPNNGQIEKRSYLRLEP
                     YAAWNFYPKSTLINKHGPSVYMSQYFDGTYLENERYLSASYTVRFQNTASTGIELINN
                     YVLLYFDQDITFSGNNALDTGGYVYNHVRGFFNSDKRKLITYTLNLDYGAYFSGKKTT
                     ADASVSWRKQPWGIFSLSARYDRIDMPEGFQDVDLLLISPRVDLSFTKSLFFTTFIQY
                     NTQIENVNINSRLQWRFRPMSDLFIVYTENYNPYNVAIKNRALAVKLVWWINV"
     gene            7886..8668
                     /gene="mnmD"
                     /locus_tag="PK610_01425"
     CDS             7886..8668
                     /gene="mnmD"
                     /locus_tag="PK610_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019668456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HRJ34611.1"
                     /translation="MLQGFDGGMLFRTFDHSKRPFFLIFVPLLKKDDLLLNMDSNLRT
                     VTTADGSVTLFLPDRDETYHSVHGAETESMHVFIKNGLEVAANDKAKLKVLEIGFGTG
                     LNALLALLFAEKNQIAIEFVSTEKYPLQKEMVLQLNYGKRLLADELFLQLHTCSWNEV
                     HPLNAHFSLFKHEGDFREANLEANSYDVIFFDAFAPRVQPELWTIEVFQQMYRLLHPG
                     GILVTYCAKGEVKRTMKAAGFSVERLPGPPGKREMTRARKSD"
     gene            complement(8731..9177)
                     /locus_tag="PK610_01430"
     CDS             complement(8731..9177)
                     /locus_tag="PK610_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRJ34612.1"
                     /translation="MTLVKKYNRFPFFPSMFDDEMLKSMSNWPGTMHHNASIPAVNIA
                     EKEDAFVIDVAVPGLSKEDFKVELSNNILTISSEKESKQENNDEKGQYTRKEFHYASF
                     TRSFTLPEKLIDQEKINGSYDNGILQLVLPKKEQTLNKGSRNIEIR"
     gene            complement(9312..10214)
                     /locus_tag="PK610_01435"
     CDS             complement(9312..10214)
                     /locus_tag="PK610_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ34613.1"
                     /translation="MKTSYFLNELQAREFLTNWNNSIIQRCGRAWKSEFVPTKWGKTH
                     VLHLHAEQEHLPALIIFPGARTSGLVWELDNAMEKIKNHRIFLIDVNGQPTLSDGNCP
                     DMKKGELGEWASQVMDALHLEQAHIAGASFGGLISLQLAVHSAKKVQSIALLNPAGLA
                     SFSMKPKNMFQNLRPLLTKKRRHVESFIQSIVLHAPEHQLENEAFQKLADYQHYVILH
                     HRFKGDYPARQPDQLLKGIQQPCLLLLGENDPLFPWQKSKKRAEKHFTKKPVVQILPG
                     IAHGIETLPLALELVNDWINNPEK"
     gene            complement(10249..10854)
                     /locus_tag="PK610_01440"
     CDS             complement(10249..10854)
                     /locus_tag="PK610_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRJ34614.1"
                     /translation="MSVSSPSKDQIIKAAGILFRKKGFKATTIREIAQKSKTNIALVS
                     YYFGDKEKLYNEILSKDIQKFRLGLSSIIHDPKSSIREKLIAFVNYYFDQLEQQPELS
                     AFVMNELQKSPGRTAGKIQKSTSFMDSVFYKQIKKEMRPAPNGKKDPMQVLVNLYALT
                     VFPFSTSEMIKRMTGSDEESTSLMISNRRAQIVEWILAIHF"
     gene            complement(10932..12758)
                     /locus_tag="PK610_01445"
     CDS             complement(10932..12758)
                     /locus_tag="PK610_01445"
                     /inference="COORDINATES: protein motif:HMM:NF018880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ34615.1"
                     /translation="MKKVFLSVTLLLIVLFAQAQYQIGQRTITFNDPSRTGGFGSGGG
                     PGRQIQSEIYYPATSNGLNTPFASGTFPVIVFGHGFVMSWDAYQNIWQNLVPQGYIMV
                     FPRTEGGFSPSHQNFALDLRVCVEKMALLNADNTSPFYQKQNGRSAIMGHSMGGGATI
                     LAANGYTAIQTIVALAPAETNPSAVSAAPGITVPALVFSGAQDGVTPPVDHHLPIFNA
                     IGSSCKTYLSIPGGGHCYFANSNFNCDFGESTSSTGISITRAQQQDVSQDHYGLWLNY
                     HLKSNCASWSQFLDSLQYSTRIIDNHLCGITVVQAQPGIISQASCGMNNGSASVIASG
                     GQAPYSYLWSNGQTTSTASSLAAGSYTCTVTDGNSCSVIVNVSISSSGGPTATITTTP
                     ANCAGGTGSFTVAPSGSGPFTIDYNGVNPASAPAGNYSIEVTDNNGCTSAFPVTISEP
                     AAISITGNANSPQSGNDGTIDLNVSGGTAPYQYSWSNGSTSEDLSGLAAGTYTVTVTD
                     NSGCTGTQSFTLNPATGTGIDEADASSLIIEQSGGSIKLISTKPIAQLQLFDISGRLI
                     WKNATIATSVEIQTGNFAFSLGVLLVEFEDGRVERYKIVVQP"
     gene            complement(12935..>13120)
                     /locus_tag="PK610_01450"
     CDS             complement(12935..>13120)
                     /locus_tag="PK610_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34616.1"
                     /translation="DMPEELCRRQLMGNVAQKPLVTEKEKISSYFRMQTDFRKLRGKI
                     HASYCIAILHKKIFLLI"
BASE COUNT         3660 a         2717 c         2852 g         3891 t
ORIGIN      
        1 taaaaagatt gttattcaga gacaaacttc gggaaaaaaa tagttaatga tcattcagaa
       61 ttaaaaccta atcctttgcg gtaaaccccc tctaggttaa atcccctgtt tacctcgtaa
      121 acgtacactt aaacaacgcc tcatttcccc tttcatccct tacacgtact tccaatgaat
      181 gtgtgccggt tgtgaccttc tcttcttcca tgttcaatat cagtttaccg gttttcactt
      241 tgtatcgaag taatttccac tctccgtcaa taaatgcctc ataggttttt acgccgctca
      301 gattatcact gactgtaaat tgcagttctt tttgtgcgct tacgtttttc ccttctgaaa
      361 tattcaccgc tgtaattact ggtgcaatag tatcaagcat tacagaatat cttccgaaac
      421 ttcttgtccg gaaggtgaca tgaccattgg aataaattcc tccttcactg cttatttttc
      481 cttttgcatt gatgtagatt ccaacggcat aaggacgaag atgttccatt aatgtgtcgc
      541 agggaagcga gatctccatg tattcctgaa ccggaatatc actgtttcca acacggaaag
      601 tgtatgctat tgcgtctttt cttcgtgtgt gtcgttcaac aaggatgcag tgattgtcgt
      661 aaattccagt agccggaatt aaaacttcca tatccataaa ccggatgatg ttgggatgat
      721 cccaatgcac agggaacaat ccgtattcct ccattgagct tttcggacaa ggatctgatt
      781 ttgccggtgg aattttagtg ctgtcactta caatggtgaa ttgaagttca gatacattcc
      841 ctttgatatc ttttaccagg tatttcattt ttattcgttg tccaggttgt accgttaccc
      901 atccgttatt cactttaaca ggataaatat ccagtgcatt atttcccttg atataacttt
      961 tgtggtaact gtttttgtta tcgcgatgtt ccagataatc catgtgcgta ttgatgtatc
     1021 tgtttgtgcg aaaatcgatg cattgcattt gttgcttcca cagcaaactg tctttcatat
     1081 acaattctac ttcgtaaact ccgcataaat tccctgatcc ggtggtcata tcgatggtgt
     1141 gtactgcaaa tcctactgta cctcttgctt tgacttcctt taccagggaa tattttccat
     1201 ttgcgccggt ggctttgatc attcgttctg aactctgacc atcaattacg ctggtatctg
     1261 taagcggata gatttttata tcatgaatgg tcggtttgat atcatctttt acatcgaatc
     1321 cgaaaagcat gggattaagt gctttttcag ttgctgtttc ccgcacttca aaatgaagat
     1381 ggggtccgcc cgaacttcct gagtttcctg accatgctat gacatcacct tttttgaaaa
     1441 caaataattc ggatggcagc gaatcgagat caacctcttc tttgcgcaac tcgtattgtt
     1501 tttcacggat gtatttttcg atcgacgaat gaaaacgcga gaggtgagca taaactgttg
     1561 tgtatccgtt ggggtgattc atgtacaata cgagtccgta cccgtagtgc gatacccgga
     1621 tgcgggagat gtatccttct gctgtagcat agatccgctg accttccacg ccttgggttt
     1681 tgatgtcgag tccggtgtga aaatggtttg ctctcagctc cccaaagctt ccggagaggt
     1741 aaagtgggat gtccattgga gagcggaaat agttttgtgg gtaggtttga gcaattgtat
     1801 tagggctaat tgcaagcgac attgccaaca gtaaaaatcc ggatagtgga ttcaaaaaac
     1861 accgttttat tttctttgtg gcttgttttt caatcattta aaatcgtgtt ttgggatttg
     1921 gtcaggtaat tgaaccttaa aggtacatgc catgaaaacc gtgccgaaac gattgcgtat
     1981 tagatttcca cagcgcttac cttaaaaatg cttcgattac gtaccgattt cctgttgata
     2041 taccctctat tttgttaatt ttgcgtagtt acttctatac cgtaacaaat taacgaacag
     2101 gatgacagcc tcactcaaag caaaagttga acgtttgatt caccattgtg atgaagtgaa
     2161 aaggtctgag ggtcagttgc aggaacgtgt taaggttctt gaggctgaga gggttcaatt
     2221 gcagcaattc attagtcagt tgcatggcga aattgaccgc agacaatccg aaatcgcaac
     2281 tttgaaccgg aatatggaag tgctgaagaa cgctgaagca ttgaaccagt cggacgaacg
     2341 atcggccgat atgaaacgta agatcaatga actgatgcgg gagatcgata actgcataac
     2401 gttattgagc aaataggtcc catgagtgaa atctccataa aggtcaacat tggaactcgt
     2461 tcctatccgc tttcggttca gccgggagag gaagaattga tccgtgcagc tgcccacgat
     2521 gtgaatgaaa acattcgttc gctgcaagag aattacgctg ttaaggatat ccaggatctg
     2581 ctggctatga ctgctcttac tatggcaacc caacttcgtc aaaaatccac cggaggaatg
     2641 tccgatgctg atgctgcgag tcttgccgat attgaacgta tgctcgacgc ttacttatcg
     2701 cgcgtttgat cttccccttg gtttgtttcg gtctgcgatt ccggtaatgt gcccggcatt
     2761 gttaaaccac tatatacaca tccacaatgg aacctatcag tttaatgatc ggcgctgcag
     2821 caggacttgt tctcggaggg atcatcattt atgtgattct gaacggggtc cttcgcaaaa
     2881 agcgtgaaga ggtcattcgc gaagccgaaa aagaaggcga gaacatcaaa aaggacaaaa
     2941 tccttcaagc gaaagaaaaa ttccttcagc taaaagagga gcatgaaaaa gcagtgatcg
     3001 aacgtgatcg caaaattcaa agtgctgaag atcgcatcaa gagcaaggaa aaagaactat
     3061 cccgcaagat ggaagatgta acacgcacag agcgtgaagc acaaagtcag cgcgatactc
     3121 tcaacgcaca gagtgaaaaa ttgcaaaagc gtcaggaaga actcgataag gctcaccaga
     3181 aacaggttgc cgaactcgaa aagatttcag gatacagcaa agaagatgca aaagctcaat
     3241 tggtagaagc actcaaagca gaagcccgta cagatgcaat ggcgcatgtg aaggagatta
     3301 tggatgaagc gaagctgact gcaaacaaag aagcaaaacg catcgttatt caaaccattc
     3361 agcgtgttgc aacagagcag gcggttgaga atgccgtttc cgttttcaat atcgaaaatg
     3421 atgaactcaa aggacgcatc attggtcgtg aaggaagaaa tattcgcgca cttgaagctg
     3481 caacaggagt agaaattgtt gttgatgata caccggaagc aattattctt tcctgctttg
     3541 atcccgttcg ccgcgaaatt gcacgtcttg cacttcacca actggtaagc gacggacgaa
     3601 ttcaccctgc gcgtattgag gaaatcgttt caaaaacgaa acataaactc gaagaagaaa
     3661 ttgttgagat cggaaagcgt actaccatcg accttggtat tcacggattg catcctgaac
     3721 tgattcgtat ggttggaagg atgaaatacc gttcttctta cggacaaaat ctgcttcagc
     3781 acagtaggga agtagccaat ctttgtgcga taatggcatc tgaacttgga ttgaatccga
     3841 agattgcaaa gcgtgcagga ttgttacacg atattggaaa agttcccgac gaagaacccg
     3901 aattgccaca tgctattttg ggaatgaagc ttgcagaaaa atttggcgaa aaggccgata
     3961 tctgcaatgc gattggagct caccacgacg aaattgaaat gaccacgctc tatgcgccta
     4021 tcgtgcaggt gtgtgatgct atttcaggag cacgtcccgg tgcgcgtcgt gagattgttg
     4081 aatcgtacat caaacgaatc aaggaactcg aagaacttgc gcaatcatat accggagtta
     4141 gtcaggccta cgctattcag gcaggacgcg aactgcgtgt gatgtgtgaa gcggagaagg
     4201 tgagtgatgc tgatgtagat aaacttgcat tcgatatttc gcaacgtatc caaacagaaa
     4261 tgacttatcc tggtcaggta aaaattaccg tgatccgcga aaagcgcgct gtcaattacg
     4321 cgaaataaca tccgggcttc agcccggatg ttatcaatgt cattaccatt tcgtatccgg
     4381 gggcttcagc ccccggtttt taaaatcccc aatccaatcc ccaatcccta attcaattcc
     4441 caatcccaat gccctctcat ccgcaacata ttctcatcac aggagcagcg ggattcatcg
     4501 gctccaactt atgcgagcac tttcttgagt tgggatattc agttacaggg atggataatt
     4561 tcctcaccgg aaaaccttcc aacatagcag gattttattc gcacccgaat ttttgttttg
     4621 tggagggtga tattcgtgat aagtcaaaat gcggtgaagc gatgaagggg atatccatcg
     4681 tattgcacca ggctgcatta ggttcagtac cgcgttcagt gaaagatccg cttactacca
     4741 attcggtgaa tgttgacgga tttctgaatg tcattcatgc agcacatcag gcaggagtaa
     4801 aacgatttgt ttatgctaca agttcatccg tctatggcga tcattccggc ttgcccaaag
     4861 tggaggatgc tattggtaaa ccactttcac cttatgcagc ttccaaatac atcaacgagt
     4921 tgtatgcagg tgtatttcat cgactgcatg gaatggaagt aatagggtta cgttatttca
     4981 atgtattcgg caaacaccag gatccagacg gtgcgtatgc tgcagctatt cctaaattca
     5041 tcaagctatt tctcgaagga aaatcgccgg tcatttacgg tgatggctcc aattcccgcg
     5101 acttccccta tataaaaaac gttattcaag ccaatgaaaa ggctgccttt accacaaacg
     5161 aaaaagcgtt tggagaagtg ttcaatattg cctttggtga acgcactaca cttttggaat
     5221 taatcacaga aataaaaaat gcactctctg ttcatgcacc aaatgtgaaa accatttcgg
     5281 tgactcatga gaatgaacgt ccgggcgaaa ttccccattc actggcaagc attgagaaag
     5341 cacggaatgt tttagggtat acacccagcc atagccttaa agcaggatta caagaagctg
     5401 tgacttggta tttagaaaat atatagcatt ttctatctat aagataataa tgcatattga
     5461 tttttttcga agaaaaatct tttttattct tgttttctaa acgttaatcc accaaaccaa
     5521 cttcactgca agcgcacggt ttttgatggc aacgttgtag ggattgtaat tctccgtgta
     5581 aacaataaac agatccgaca tcggcctgaa tcgccattgg agtcgggaat tgatatttac
     5641 gttttcaatc tgcgtgttgt attgaataaa tgtagtgaag aacaaacttt ttgtaaatga
     5701 aagatccaca cgcggactaa tcagcaacag gtcaacatcc tgaaatcctt ccggcatatc
     5761 tattctgtcg taccgtgcag aaagcgagaa gattccccag ggttgtttgc gccagctgac
     5821 agacgcatct gctgtcgtct ttttaccgga gaaataggcg ccgtaatcga ggttaagcgt
     5881 ataggtgatg agtttacgtt tatcagaatt aaaaaagccg cgtacatgat tgtaaacata
     5941 tcctcctgta tccaaggcat tgtttcctga aaaggtgatg tcctgatcaa aatacaggag
     6001 cacataatta ttgatgagct ctataccggt tgatgccgtg ttttgaaaac gtacagtata
     6061 gcttgcactc aaatagcgtt cgttttcgag ataggtacca tcgaaatact gactcatata
     6121 aaccgatgga ccatgtttgt tgatgagtgt tgatttcgga tagaaattcc atgcggcata
     6181 tggttctaat cggaggtagg atcgtttttc aatctgtccg ttattgggat taaaaaaatc
     6241 gatacgggga acaaagccaa catcggcgga gtaattgtga ccaacatatt cgtgattcca
     6301 ttcgagaaca agttttggag tttcgtaatg tatccatgag gcatgtgcca ttgcattttc
     6361 cgttttgtgc ggagaaaacg aatgatgcat gaaaattttc cccattaaac gattgtccat
     6421 tgttgcaatg ttccagtcga tacccgctat gcgattgtaa tccgcgaaat tcatgtcttg
     6481 cttaatgaat ccttgtctgt tcaccagtat tcccgaaata ttggagcggc ctttcatctg
     6541 gtacgtgact gcagcaacag aatagttttg tccttcaagt ccactttgcg atccgccttc
     6601 ggtttgcatg gtcattgcac caattctcca gtcgcgattg atttttccac tcaaacgggc
     6661 accaaaaaga atgggaacag cttctccgtt attcaaaccg atgcgtcgtg aaaaaaaggg
     6721 acgaatctgt cggaatccga atcgctcgaa gagatcgctg ttttcaagaa agaaatttct
     6781 tcgttcggga aaaaataaac tgaaacgtga aaggttcgtt acctgacgat ccacttctac
     6841 ctgcgcgaaa tcgggattga tggtgagatc caggttcaac gatgaattca ccgcgatttt
     6901 tgcatccaaa ccggcattgg gacgaaattt ttcagcagtc tggttttggt aatttccggt
     6961 tgctgttcca atggcatatg gaatcaagga aacatttgaa ccttctttgt gtggtgcttc
     7021 ttctagtagt agttttccgg taaatgcaag tgaagaagtg gaaaaattac gaggtactcc
     7081 tgaccatacc gaaatttcat tgcgtttaaa atcgtagcgc gcaaaattga ttcgccattc
     7141 tttgatgttt tcaggaaaac gcaatgtttt ataaggaatc gccatctcaa cgatccattt
     7201 tccttcggaa tgatattgtt tcacttctga gaaccattta ttatcccaaa ctgtggagat
     7261 gcccatattg ccaccatatt gaatgagtcc ttccatctgg gcaccgtgcg gatttacgcc
     7321 aaatacaaat ccattggtca gatcagaaaa aggatcgatg taaacataga atccatcact
     7381 cagcagagga tcgtaatccc gtttgagcga ttgtataaca tgctctccgg gtaattcatc
     7441 atagcaaatg gcagcaatgt ggagatgatc tttatcaaac gcaaccatca cttccgtttt
     7501 cgatttcgcc atggaggaat caaatggaaa ccactgacga aaatcggaaa tgatcatggc
     7561 atttttccag gcatcatcct caagaagtcc atctatcttt atgatttgtt cagatttttt
     7621 tgctgaaaca ctatgcagtt cctgcgcatg caaaagcgaa ttgcagaaaa aaagtagcac
     7681 tgtcagaagt ccggtccttg tcattgggac cgaaaattac taaaaaacaa atcccctgtg
     7741 agccattccc actaaggatc tgtttttctt cgacaatatt gccttaacac acgataattg
     7801 ccataaccgt gaagattccc accacgaaaa gagccataac tgttcctttg tccataatac
     7861 acgttttgct tataaaacgc agctaatgtt gcagggtttt gatggtggaa tgctgttccg
     7921 gacctttgac cattccaaac gaccgttctt ccttatcttt gttccattgc ttaaaaaaga
     7981 cgacctttta ctcaatatgg attccaattt acgtacggta actacagctg atggatcggt
     8041 aacccttttt cttcccgacc gcgatgaaac ctatcattca gtgcatggtg cagaaacgga
     8101 atccatgcat gtatttatta aaaatggtct tgaagttgcc gctaatgata aagcaaaatt
     8161 gaaagtgttg gaaattggtt ttggtaccgg attgaatgct ttactggcct tgttgtttgc
     8221 ggagaaaaat cagattgcaa tcgaatttgt aagcacagaa aaatatcctc ttcaaaaaga
     8281 aatggttttg cagttgaatt atggcaaacg attattggcc gatgaattgt ttttgcaatt
     8341 gcacacctgc agttggaacg aggtgcatcc gttaaatgcg cattttagtt tattcaaaca
     8401 cgaaggagat tttcgtgaag ccaatcttga ggccaactct tatgatgtca tttttttcga
     8461 tgcatttgct ccacgggtac aaccggagtt gtggacgata gaagtatttc aacagatgta
     8521 tcgcttattg catccgggtg gaatccttgt cacctactgt gcaaaaggcg aagtcaaacg
     8581 cactatgaaa gcagcgggat tcagtgtaga acgattgccg ggacctccgg gtaaacgtga
     8641 aatgacaaga gcgcgtaaat cggattgatg cgtagaaaaa aaacggactg ttccattgcg
     8701 aaacagtccg ttctctttta gaatttatcc ttagcggatt tcaatgttgc ggcttccttt
     8761 attaagggtt tgttcttttt tgggaagaac caattgaagg atgccgttat catagcttcc
     8821 attgattttt tcctgatcga tcagtttttc aggaagggta aacgaacggg taaacgacgc
     8881 ataatggaat tccttgcgtg tgtattgtcc tttttcgtcg ttgttttcct gtttgctttc
     8941 cttctcactt gaaatagtaa gaatgttgtt gctgagttcg actttaaaat cttccttact
     9001 caatcccggt actgcaacgt caattacaaa tgcatcttct ttttcggcga tgttcactgc
     9061 agggatggat gcattatggt gcatggttcc gggccagttg gacatagatt ttagcatttc
     9121 atcatcgaac atggatggga aaaagggaaa gcggttgtac tttttaacga gtgtcatggc
     9181 tattgttttt taggttatac atgttttggc atgatacctc aagacaaatg ccgatagtgt
     9241 ttttcggaaa ttttagcagg attctgcaag ttctgcctgc aaatttttca ggttttacgc
     9301 tgtttatctg actatttttc aggattgttg atccaatcat tgaccaattc aagcgccaac
     9361 ggcaaggttt caattccatg cgcgatacct ggaaggattt gcaccaccgg tttttttgtg
     9421 aaatgctttt cagccctctt tttgcttttt tgccagggaa ataaaggatc attttctccc
     9481 agcaataaca aacaaggctg ctgaattcct tttaacaact gatccggttg tcgggcgggg
     9541 taatcgcctt tgaaacgatg atgaagaatg acataatgct ggtagtcggc aagtttttga
     9601 aatgcctcat tttcgagttg gtgttcggga gcgtggagga cgatggattg aataaacgat
     9661 tcaacatgac gtcttttttt tgtgagtagc ggacgtaaat tctgaaacat gttttttggt
     9721 ttcatggaga aggatgccaa tcctgcagga tttagcaaag caatactttg aacttttttc
     9781 gctgaatgta cagccagttg caaagagatc aatcctccaa atgatgcgcc tgcaatatgt
     9841 gcctgttcaa gatgtaatgc atccattacc tgacttgccc attcgcccaa ttctcctttt
     9901 ttcatatccg gacaatttcc atcgcttaat gtcggctgac cgtttacatc gatcaggaat
     9961 attcggtgat tctttatttt ttccatggca ttgtccaatt cccaaaccaa tccgctggta
    10021 cgtgctccgg gaaaaatgat cagcgccggt aaatgttctt gctccgcatg aagatgcaat
    10081 acatgcgttt ttccccattt tgtaggtaca aattccgatt tccatgctct tccgcatcgt
    10141 tgtataatgg agttgttcca gttcgtaaga aattcccttg cttgtagctc atttaaaaaa
    10201 taggaagttt tcattttaaa cagttgttta aattataact aaaaaaaatt agaagtgaat
    10261 agccaatatc cactccacga tttgtgctcg gcggttgctg atcattaagg atgtagattc
    10321 ttcatcgctg cctgtcattc gcttgatcat ttctgaagta ctgaagggaa atacagtgag
    10381 cgcatatagg ttcaccagca cttgcatagg gtcctttttt ccgtttggag caggacgcat
    10441 ttcctttttg atttgtttgt agaacacaga atccataaac gaggtgctct tctgaatttt
    10501 acccgccgta cgaccgggag acttctgcaa ttcattcatc acaaaagctg aaagttcagg
    10561 ttgctgttca agctggtcga aataataatt tacaaacgca attagttttt cccggattga
    10621 acttttggga tcatgaatga tactgctcaa tcctaatctg aatttctgaa tatccttgct
    10681 cagaatttca ttgtacaact tttccttatc accaaaataa taggaaacca aggcaatatt
    10741 cgtcttgctc ttttgtgcaa tctcccgtat ggtagtagcc ttaaatcctt ttttccgaaa
    10801 caggattcct gcagctttta tgatttggtc ttttgatgga gaggatacgc tcatgaatgc
    10861 aaatttaaac atttgtttaa atcaggtcaa aaaaaaagca ccactaatgt gatgctttga
    10921 taattaatga attagggttg tactacgatt ttgtatcgtt caacccgacc atcttcaaat
    10981 tccacaagta atacccccag ggaaaaggca aaattaccgg tttgaatttc aacggatgta
    11041 gcgattgttg cgttcttcca gatcagtctt ccactgatat cgaacagttg caactgtgca
    11101 attggttttg tgctgatcag tttgatacta ccgccggatt gttcaataat tagtgatgat
    11161 gcatctgctt catcaatacc tgtaccggtt gcaggattga gggtgaagct ctgagttccg
    11221 gtgcatcctg aattatctgt tactgttact gtatatgttc cggcagcaag tccggataaa
    11281 tcttcagaag tggaaccgtt cgaccatgaa tattgatacg gtgcagttcc tccgcttaca
    11341 tttaagtcga tggtgccgtc gtttccactt tgtggtgaat tggcattacc tgttattgat
    11401 atcgcagctg gttcgctgat cgtaaccgga aatgcagatg tacagccatt gttatctgtt
    11461 acttcaatcg aataatttcc tgcgggagca cttgccggat ttacgccgtt gtagtcgatg
    11521 gtaaatggtc cgcttcctga aggtgccaca gtaaatgatc ctgtgccgcc tgcgcaattg
    11581 gcaggggtag tggtaatggt tgctgttgga ccaccggaag aggatatgga tacattgaca
    11641 ataacagaac atgaattccc gtcggtaacc gtgcaggtat aagatcctgc cgcaaggctg
    11701 cttgctgttg aagtagtttg accattgctc cagaggtacg aatatggcgc ttgtccgcca
    11761 cttgcaatta cggatgcaga tccgttattc attccacaac tggcttggga aataatgccg
    11821 ggttgtgcct gtacaactgt aatgccacat aaatgattgt cgataatgcg tgtggaatat
    11881 tgcagcgaat cgaggaattg cgaccaggat gcacaattgc tttttaaatg atagttgagc
    11941 cataatccgt aatgatcctg actaacatcc tgctgttgtg ctcttgtaat agaaattccg
    12001 gtagaggaag tggattctcc aaaatcacaa ttgaaattgc tattggcaaa ataacagtgt
    12061 ccgccgccgg ggatggatag ataggtttta cacgatgaac cgattgcatt aaaaatagga
    12121 agatgatgat cgacaggtgg tgtcacacca tcttgtgctc ctgaaaaaac aagcgcaggt
    12181 acagttatcc cgggtgcagc gcttaccgct gaaggattgg tttctgctgg tgctagtgct
    12241 actattgttt gaatagcggt atatccgttt gctgcaagta tggtggctcc tcctcccatt
    12301 gaatgtccca tgatcgcact tcttccgttt tgtttttgat agaacggtga ggtattgtcg
    12361 gcattcagca gcgccatttt ttcgacacaa acacgaagat cgagtgcgaa gttttggtga
    12421 cttggactaa atcctccttc tgttcgcgga aataccatga tgtaaccctg gggaacaaga
    12481 ttctgccaga tgttttggta agcatcccaa ctcatcacaa agccatgtcc aaaaacaatc
    12541 accggaaatg ttccacttgc gaatggtgtg ttaagtccgt tgcttgtagc aggataataa
    12601 atctccgact gaatctgacg gccgggacca cctcctgaac caaatcctcc tgtgcgcgaa
    12661 ggatcgttga acgtaatagt gcgttgcccg atctggtatt gcgcctgggc aaacaaaacg
    12721 atgagaagaa gggtgacaga aagaaaaacc tttttcattt tttaattttt tggcaatatg
    12781 gaccttcctt tcccggtggg caagtggtgg tgtaatccaa atatcagatg aaccgcttgt
    12841 taagttcagg acaaattatt catccgattg tttaaaattc cacgaaacca ttattcggga
    12901 catgaattcc aaaatccatt tacatgctta ctattcagat gagtaagaaa attttcttgt
    12961 gaagaattgc aatacaataa ctggcgtgga ttttaccgcg taattttctg aaatcagttt
    13021 gcatacggaa ataagaactg attttttcct tttcagtgac aagtggcttc tgcgcaacgt
    13081 tgcccatcaa ttgccgccga cacaattcct ccggcatatc
//
LOCUS       DAOMYG010000022        12954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1613_length_12954_cov_8.912706, whole genome shotgun sequence.
ACCESSION   DAOMYG010000022 DAOMYG010000000
VERSION     DAOMYG010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 12954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12954
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1613_length_12954_cov_8.912706"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..2915
                     /locus_tag="PK610_01455"
     CDS             186..2915
                     /locus_tag="PK610_01455"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRJ34617.1"
                     /translation="MIQDRSQNYYISTNEGVYQYDFYSYKKLNCPDCKGNSFFNFTSN
                     SSGVIYCNNLNKQIYKIEKGRLELFYELQSDEESSDLSLMIAPNGEILVSGHKIVVLD
                     SVGKVLTRKMISLRYIGQPYINKKNEVIYHLFGSDSLIIYYNNKIRCEKLISSSEISA
                     GLRFFRLGEKELAIDTETKNQYSYNSTTQSLTPFTKNKLFERTEAFRIYETGEDIWLA
                     GTLPGVSRFPKNSYKDIIFEDYFISDVYRDSEGNLLFSTFDKGIFVVPDKDLYDVIFN
                     SKEDPVVSLYFHPASGLIAGTSQGHLIQIKGQQLNLLNTKGTRPIEVIAGNPEGTSIL
                     FDNGHIRLYDFKKATFSDIISASLKDVVAISDQEYVLGTNRGIIRLLLIRNGKFNIEW
                     VEDAKFRIYSLEYHPIEKSIYASTSEGFICLAENNKVGKVLNKGNDHYSNFVHYSNGI
                     IYSAGKDGSILKIKKGEIIGRIPLTKKDETFEIQKFAIYKNTFLVKSPKGFDQFNSDG
                     KYVRSLQNTFFLPENRIIDFTVYGDSVWISHDNGIQHFNLNYLDKQQDKPEIVLQQIT
                     LNDNPIAIDKPGEFANDERKLRFVLSSPTLKHQSTTSYSFKLEGYDENWNTQTFNNNE
                     ITYTALAPGKYTFRAKAINQESESKEISFQFSIALPVYLKWWFMLLAGALFTIATILI
                     YKRQIRIQQKKSAQINELHLSKLAAIQSQMNPHFIFNSLNSIQDLILKGDVEHSYSYI
                     TTFSNLVRKTLNYSEKDFIDFEEDLQLLELYLSLEKLRFKKDFSYSIDHNDIEDILIP
                     PMLIQPFVENALIHGLLHKEGEKKLSLKFELKDVLICEITDNGIGREASMALQKRQRA
                     GHESFAGKAIRNRFEILSEIFDGEFDYRHEDLTENELATGTRVILRIPIKHKY"
     gene            2991..3734
                     /locus_tag="PK610_01460"
     CDS             2991..3734
                     /locus_tag="PK610_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRJ34618.1"
                     /translation="MKKIKAIIVDDEDSARDVLHNLLLRFCPEVEILAKCENVIQAVE
                     AIKNTPPDLVFIDIEMPNYAGYEIVKFFDSISFEIVFVTAYDKYALRAFEIAAIDYLL
                     KPVDIDRLKKTVQRVSERKNLQQQTERLAVLHRSMESKEMKNIVITDKGQQHVIALES
                     IIAIEAMESYCSIHTTEKKYTVSKNLKHFENLLEDSEQFIRVHKSWMIGRNHIKKYSL
                     TDLNIFLSKGLVAKLSKYKKSGFEDSLNK"
     gene            3876..5696
                     /locus_tag="PK610_01465"
     CDS             3876..5696
                     /locus_tag="PK610_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ34619.1"
                     /translation="MKLLKRLLIYARPYHHFLPEYLIYTFFGIVFGLLNFTMLIPLLN
                     LLFGNGEQTTVIPAEPSFSVSISYLIDLFNYHFLSIAILKGKMAAMAFVCVIIGAGTV
                     LSNFFRYMSMRVLVRLKLKMLEKLRVSLYDKLLDQSQRFFHSRKKGDILSTLTNDVQE
                     IEYSVISSFQTLLRDPFVIIAYFIALFYLSPSLTLFTLFFFPVSGILISQISKRLKKK
                     GYFNQEMLGKILHTSDESITGIRIIQAFRAGDRFKKNFRDLNGQFTRSSKALFNQREI
                     ASPLSELLGVIVIIVVVMYGGNLVLQGSLSGSMFITYLALYSQILQPAKNLGTAITNM
                     QRGLVATERVFNIIDEPVSIMEKTNAIQAGDFSNEIRFENVVFAYDESSVLNNISFTI
                     PRGKMIALVGKSGSGKSTLADLLLRFQDVNEGKVLIDGIDIRDITLESLRGQIGLVNQ
                     EPILFNDSILNNITMGAEADMEKVKSAARMANASEFIEQLEEGFETKVGDRGSRLSGG
                     QKQRIAIARALYKNPPILILDEATSALDTESERLVQDALHHLMENRTTLVIAHRLSTI
                     QHSDEIIVLDAGRIAERGTHAELSAKNGLYRKLCDLQSFE"
     gene            5869..7242
                     /locus_tag="PK610_01470"
     CDS             5869..7242
                     /locus_tag="PK610_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34620.1"
                     /translation="MKNILVSSVLFLLSVGVNAQALEFNFASAVPVGCSNAKPITDGV
                     KKITVDLATGNAVFSMQSLNCKKDIKAENLGVVNESSKATAYFDEKSEKGIIIEANEK
                     AVVFEKNQNAYVVTGVGHVDKKIAKTLSIETEKTTYNSVLADIPNLITKAKIRKKEAE
                     MAAKMAKLPETEYKDEYDISGIYYLSRTIHDPKSDKYVNAINFQFIVAENKALLHYHE
                     GLKNEVFIEGIYRKALQAKSLSYIYFMGDNMNSLEPFANEIFMPIEKDVYYVSGDQFG
                     TDTRLDCSSVKLTTNKDASGNIIRDYLIIGKDKKRVEELNNNPELLEKLLIKSCIEMC
                     EAYNVVRAGRNPMPGEGRVDEKLASEAFELTKKHAASKNWPQKLEYCYIKSPEWVIIR
                     NKVTGVILSRSTRVVVIMTSNGKCMWEEMEIRQDYDGAQFGKTYFYGNSGNIVPAECT
                     DAMKKKK"
     gene            complement(7294..8286)
                     /gene="dusB"
                     /locus_tag="PK610_01475"
     CDS             complement(7294..8286)
                     /gene="dusB"
                     /locus_tag="PK610_01475"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRJ34621.1"
                     /translation="MVKIGNIDLGAFPLLLAPMEDVSDPPFRRLCKKYGADLMYTEFI
                     SSEGLIRDAWKSRKKLDVFDYERPIGIQIFGGEESSMVQAAQICESAGPELIDINYGC
                     PVKKVVCKDAGAGILKNIPKMMRLTEAVVKAVKLPVTVKTRLGWDDDTKNILEVAERL
                     QDIGVQALSIHGRTRVQMYKGEADWTLIGQVKENPRIKIPIFGNGDVDSPAKALEMKN
                     RYGVDGIMIGRASIGYPWIFREVKHFMETGETMAPPSLIERVEAAREHLEFSIRWKGE
                     REGIVEMRRHYANYFRNIPHFKEYRSRLVTMLQPADVYAVLDEVLEAFSGEIIQ"
     gene            8317..9240
                     /locus_tag="PK610_01480"
     CDS             8317..9240
                     /locus_tag="PK610_01480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRJ34622.1"
                     /translation="MKTPNIRHLNPMLQMFVLFGLIFITLFLVMLLGKLVADWGYGIN
                     LLDPDFAKDPSAEGVTAVQRWLMVLNQLGFFLIPALLFGQMMYSEEQSYFPLKKPNLM
                     QFGLAIVLVFAMQTPIDFLLRLNSSLPLGDEVMSDINEMEVKAAFMIKQLIGEGSFAD
                     VAIAMIILAIIPAIAEEFFFRGVLLQTFRASFRNAHLAVWMTAIVFSFIHMQFLGFIP
                     RMMLGALLGYLMVWSGSIWLSIIVHFFNNALGVFLMFQFGAEVNTISQQAETNWLMLS
                     VGSLFTIGTIYFFHRFRLKDQNHQEPFNTFE"
     gene            complement(9224..9907)
                     /locus_tag="PK610_01485"
     CDS             complement(9224..9907)
                     /locus_tag="PK610_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRJ34623.1"
                     /translation="MKTNRNSEINAGSTADIAFLLLVFFLLVTTMETEEGIARRLPQK
                     VETEIEKEIHERNVLDIRINAANQLLVEDKITTIPELKAVLRDFLTNPKRERSLAEMD
                     FVNEEICLQNWSEIKADDSRKHEEEFWKDRLAMVQQTGAGYFICKDAVISLQNDNATS
                     YAFYIAVQDAIEQCILSLRDEWSMKLYGVAYVDLDQKDALSKERMKVIRSIVPQRLIE
                     REPLAIQMY"
     gene            complement(10056..10532)
                     /locus_tag="PK610_01490"
     CDS             complement(10056..10532)
                     /locus_tag="PK610_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRJ34624.1"
                     /translation="MSKFKKDDSSGSPAISTASLPDIVFMLLFFFMVTTVMREVDLKI
                     EIKKPEAEAITKLEKKSLVDYIYIGPATMRYRSMYGTEPRIQLDDQIATQATDVGPFV
                     IRKRTERNETDIPFVTTSLKVDEKTRMGIVSRVKHELRQVDALKINYSTTRKRVQP"
     gene            complement(10551..11273)
                     /locus_tag="PK610_01495"
     CDS             complement(10551..11273)
                     /locus_tag="PK610_01495"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRJ34625.1"
                     /translation="MAKRKLEEINAGSMADIAFLLLIFFLVTTTMDTDEGIVRSLPQK
                     LPPDYKMEVEINERDVFEVNLNSNDQLLVESDYIQIGELKGKIREFLTNPYNSENLPL
                     MEEITVERARQEIANRKQLIDNPKTPAASLGVIKSELSDWEDKLAAAEKVGPFRTLPK
                     LAIISLRNDNGTSYKGYVEVQDQMQSAINELRNEWSQKVYGENFTDWNSSNDSDKEKI
                     KVIRAIVPQRIAEQEPKNIPAY"
     gene            complement(11285..11884)
                     /locus_tag="PK610_01500"
     CDS             complement(11285..11884)
                     /locus_tag="PK610_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34626.1"
                     /translation="MKDTKLHSILTIFKGVLMVLGFVFGILLMLQGTAAVLGPGHLAD
                     EEKTLKENVGIWEGKLAGAADENEKLAINSHIDAINSNISFNGKLTFTMNLTIYMIYG
                     GLIFAILFAVYLTFIDLRNNIPFLVGIAIFGAIVLFSWVTASDAMIESWKYRDPELFN
                     PGFVKFSDFSIKLVSIFGLLAVVLIAAGWVWGLVKRFSA"
     gene            complement(11920..12741)
                     /locus_tag="PK610_01505"
     CDS             complement(11920..12741)
                     /locus_tag="PK610_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRJ34627.1"
                     /translation="MKKILSLIAISGLLTIGATQYAKAQNTDSTSATETTTPAETPAE
                     PTEATASGENADEAPKVTFTQVLKEKFIEGDPRFMSLVLICLILGLAISIERIIYLNM
                     ATGNSEKFLEKVEAALKSGGVEAAKEICRNTRGPVASIAYQALMRVDEGPDNVEKAII
                     SYGSVQNGQMENGLTWLSLFIALAPMLGFLGTVIGMIQAFDDIARAGNISPQIVADGI
                     KVALLTTVGGLIVAMILQLFYNYLLSKIDNLVIDMENSSISIMDILLKHKVFPGK"
BASE COUNT         3996 a         2717 c         2526 g         3715 t
ORIGIN      
        1 catgcaacta tccatccttt tttaagttga ttccttacca aacaaaacac cggaaaacta
       61 acagaacacc aaaagttttt tggggggtgg tttttctttt cattttaatt ttttccggca
      121 aggcccaaca acctgcctat ttcataatgg gacaggaaca atttaagggt gtcaaaatct
      181 acgatgtgat tcaggatcga tcccagaatt attatatatc caccaatgaa ggagtttacc
      241 aatacgattt ctattcatac aaaaaactga attgtccgga ctgcaagggc aactcctttt
      301 tcaatttcac ttccaactct tccggggtca tttattgtaa taatctcaat aagcagattt
      361 ataaaatcga aaaggggaga ttagaattgt tttatgagct tcagtccgat gaagaaagtt
      421 ccgatttaag tcttatgatt gctccaaatg gggagatatt ggtaagcgga cataaaattg
      481 tagtacttga ttccgttggg aaagtcttaa cccgcaaaat gatttcattg agatacattg
      541 gccagcctta cataaacaaa aagaacgaag ttatatatca tcttttcgga tctgactcgc
      601 taattattta ttacaacaat aaaattcgct gtgaaaaatt aatcagctca tccgaaattt
      661 ctgcgggcct gcggttcttt cgattaggag aaaaagaatt ggcaattgat actgaaacta
      721 aaaatcagta cagctacaat tcaacaacac aaagcctcac tccgtttaca aaaaacaaac
      781 tatttgaacg aacagaagca tttcgcatct atgaaaccgg agaagatata tggttagccg
      841 gaacgcttcc cggggtttcc agatttccaa agaatagtta taaagacatc attttcgaag
      901 attactttat atctgatgtt taccgtgatt cagagggaaa tttattattc agcacattcg
      961 ataaaggaat ttttgttgtg cctgacaagg atttatatga tgtcattttt aattccaagg
     1021 aagatcctgt agtatccctc tattttcacc ctgcatccgg attgattgca ggaacctcac
     1081 aaggacatct gattcagatc aaaggacaac aattaaacct actaaataca aaaggtacgc
     1141 gtcctattga agtaattgca ggcaatcctg aagggacaag tatcttgttt gataatggac
     1201 atatccgatt gtatgatttt aaaaaagcaa ccttcagcga catcatttct gcttcgctaa
     1261 aagatgtggt tgcaattagt gaccaggaat atgtacttgg aacaaatcgg ggaatcatcc
     1321 gattgctcct tatcagaaac gggaaattca acattgaatg ggttgaggat gcaaaattca
     1381 gaatctattc tttagagtat catcctattg aaaaatcaat atacgcttca acctcagaag
     1441 gatttatttg tcttgccgaa aacaataagg ttggcaaagt tctgaataaa ggaaatgatc
     1501 attactcgaa ttttgttcat tattcaaacg gaattatcta ttcagcagga aaggatggat
     1561 cgatactcaa aataaaaaag ggggaaataa ttgggcgaat tccccttact aaaaaggatg
     1621 aaacttttga aattcaaaaa ttcgcgattt ataagaacac ctttctggta aaatctccaa
     1681 aaggattcga ccaattcaac agtgatggaa aatatgtccg ttcgttgcaa aacacatttt
     1741 ttttgcctga aaaccgaatc attgatttta ctgtgtatgg ggactctgtc tggataagcc
     1801 atgacaacgg cattcagcat ttcaatttaa attatctgga caagcagcag gacaagccgg
     1861 agattgtcct gcaacagatt acactaaatg ataacccaat cgcaatagat aagcccggag
     1921 aatttgcaaa cgatgaaaga aaattgaggt ttgtgctttc atcgcctacc cttaaacatc
     1981 aaagcacaac aagttacagc tttaaactcg aaggttatga tgaaaactgg aatacacaaa
     2041 cattcaacaa caatgaaatt acctataccg cactggctcc gggaaagtac accttcagag
     2101 caaaggccat taatcaggag agcgaaagta aagagataag ttttcaattt agcattgcac
     2161 ttcctgtata cctcaaatgg tggttcatgt tattggccgg ggcactattt accattgcaa
     2221 caattttgat ttacaaacgt caaatcagga ttcaacaaaa gaaatcggcc caaattaatg
     2281 aactccattt atcaaaactc gctgccatac aatcgcagat gaatcctcat tttattttca
     2341 attcactgaa ttctatccaa gacctcatcc tgaaaggaga tgttgagcat tcttattcct
     2401 acatcactac tttttcaaat ctggtaagaa agacgctcaa ctattcagaa aaagatttta
     2461 tcgactttga agaagaccta caattgcttg aattgtattt atcgctcgaa aaactgagat
     2521 tcaaaaagga ttttagctat tcaattgatc ataatgatat tgaagacata ctaataccac
     2581 caatgctcat tcaacccttt gttgaaaacg ctttaattca cggtttgctg cacaaagaag
     2641 gcgagaagaa attaagtctg aagtttgagt taaaagatgt actgatttgc gaaattaccg
     2701 acaacggaat aggaagagaa gcttccatgg ccctgcaaaa aagacaacgg gcaggtcatg
     2761 aatcctttgc aggaaaagcc attagaaacc gtttcgaaat tttgagtgag atttttgatg
     2821 gagagttcga ttacaggcat gaagacttaa ccgaaaacga gctagctacc ggaactcggg
     2881 ttattttaag aattccaatt aaacacaaat actgattcgt tgctgaataa aacaaatcca
     2941 ttcatgaatt taccgatttg cggttaagtt aaaacacgta cttgcaggca atgaagaaaa
     3001 taaaagcgat aattgtggat gatgaagaca gcgccagaga tgtcctccac aatcttttat
     3061 tgcgtttttg tccggaagta gagattcttg ccaagtgcga aaatgtaatt caggcagtag
     3121 aagccattaa aaacacccct cccgacctcg ttttcattga catcgaaatg ccgaattatg
     3181 ccgggtatga gattgtgaag ttttttgata gcatttcgtt cgaaattgtc tttgttactg
     3241 cctacgataa atatgcttta cgggcatttg agattgctgc catcgattac ctgttaaaac
     3301 ctgtagatat tgaccgctta aaaaaaacag tacaacgtgt ttcggagcgt aagaatttac
     3361 agcaacagac agaacgcttg gcggtgctac accggtcgat ggaaagcaag gaaatgaaaa
     3421 atattgtaat aacggataaa gggcaacaac atgtaattgc gcttgaatcc attattgcca
     3481 tcgaagcaat ggaatcgtac tgctcaattc atacaacaga aaaaaaatac accgtaagta
     3541 aaaacctaaa gcactttgaa aatctcctag aagattctga gcaatttatt cgggtgcata
     3601 aatcatggat gatcgggaga aaccatatta aaaaatactc acttacagat ctcaatattt
     3661 tcctgagtaa gggcttagtg gccaaacttt cgaaatacaa aaaatccggg tttgaggaca
     3721 gtttgaataa ataatcaaca ctcctaaata ttggtcgatt aagatgacaa catttttctt
     3781 caggccaaat gcggcacccc cctcgaaatt agccatttat gtctctaatg gcaatattta
     3841 ctaccttgca gcgttttcaa atccgataaa cgcttatgaa gctgctcaag cgacttctta
     3901 tttacgcccg gccttatcat cattttttgc cggaatacct catctacacc ttttttggta
     3961 ttgttttcgg actactcaac tttacaatgc ttattccgct gctcaatttg ctttttggga
     4021 atggagaaca aaccactgtt attcctgcgg aaccctcctt ttcggtttcc ataagctacc
     4081 tgattgactt attcaattat cattttctga gcatagccat tctaaaaggc aaaatggcag
     4141 caatggcatt tgtatgtgtt atcataggcg caggcacggt gctttccaat tttttccgct
     4201 atatgtcgat gcgcgttttg gttaggttaa aattaaaaat gctcgaaaag ctccgtgtat
     4261 cgctctatga taaattactg gatcaatcac aacgtttttt ccattcccgc aaaaagggag
     4321 atattctttc taccctcacg aatgatgtac aggagatcga atattcagtc atatcatcct
     4381 ttcaaacgct tctgcgagat ccttttgtga tcattgccta tttcattgca ctattctacc
     4441 tttccccctc gcttactctt ttcacgctct tcttttttcc cgtttccgga attctcattt
     4501 cccagatttc gaagcgactc aagaaaaaag gatacttcaa tcaggaaatg cttggaaaaa
     4561 ttctgcatac atctgatgag agcattacag gcattcgcat tatacaggcc tttcgtgccg
     4621 gagatcgttt caaaaagaat ttccgggatc tcaacggaca attcacccgc agcagtaaag
     4681 cgctttttaa ccaacgtgaa attgcatctc cacttagcga acttctgggt gtaattgtaa
     4741 ttattgtggt ggtgatgtat ggcggaaatc ttgttttgca gggaagtctc agcggctcta
     4801 tgtttattac ttacctggct ttgtattcgc aaatactgca acctgcaaaa aacctcggca
     4861 cagctattac gaatatgcaa cgcggactag tagctacaga gcgtgtgttc aacattatcg
     4921 acgaaccagt ttccataatg gaaaagacga atgccattca agccggtgat ttttcgaacg
     4981 agatccgctt tgaaaatgtg gtgtttgcat acgatgaaag ttctgttctg aacaatatta
     5041 gttttacaat tccccgcgga aaaatgattg cactcgtcgg gaaaagcgga tcagggaaat
     5101 caacacttgc tgatcttttg ttgcgttttc aggacgtaaa cgaaggaaaa gttttgatcg
     5161 atggaattga tatccgtgat attaccctcg agagtttacg cggtcagatc gggcttgtaa
     5221 accaggaacc cattctattc aacgattcaa tactgaacaa tataaccatg ggagcagaag
     5281 ccgacatgga aaaagtaaaa tcagccgcgc gcatggcgaa tgcttccgaa tttattgagc
     5341 agctggaaga aggattcgaa accaaagtcg gcgacagagg ctcgcgtttg agtggtggtc
     5401 agaaacaacg tattgcgatt gcgagggcgc tttataaaaa cccaccgatt ctcatcctcg
     5461 atgaagccac ttccgcactc gataccgaat cggagcgact ggttcaggac gcattgcatc
     5521 atctgatgga aaaccgcaca accttggtca tcgcgcaccg actctctaca attcagcatt
     5581 cagatgagat aattgtgctt gatgccggaa ggatagcaga gcgcggaaca catgctgaat
     5641 tgtctgcaaa aaacggtctc taccgaaaac tttgtgatct gcaaagtttt gagtgaactt
     5701 tttcacttca agtacctcgt cgggaagaaa tgcacaccgt agttatttca agaaaagaac
     5761 agtaatattc ggttcacaaa gtcatatcca ctgctcacaa aaaaggaacc ggaacatact
     5821 ggtatctgca atagatttgg tgaaaacaat taatcgaccc aaatctttat gaaaaacatc
     5881 ctagtttcat ctgttttatt cctcctttca gtaggagtaa atgcacaagc cctcgaattt
     5941 aattttgctt cagcagtacc ggtaggatgc agcaatgcaa aaccaattac agatggcgtg
     6001 aaaaaaatta cagttgatct tgcaaccgga aatgccgttt tttcgatgca aagcctgaat
     6061 tgcaaaaaag acattaaagc agaaaacctc ggtgtagtca acgaaagctc aaaagcaacg
     6121 gcctatttcg atgaaaagtc agaaaaagga atcatcattg aagcaaatga aaaagcagtt
     6181 gtgtttgaga aaaatcaaaa cgcctatgtg gtaaccggtg taggacatgt cgacaaaaaa
     6241 attgcgaaaa cgctttccat agaaacagaa aaaaccactt ataattccgt gcttgctgat
     6301 atacccaatc ttatcacaaa agcaaaaatc agaaaaaaag aggcagagat ggcggctaaa
     6361 atggccaagc ttcctgaaac agaatacaag gacgaatatg atatttctgg gatctattac
     6421 ttgtccagaa ctattcacga tcctaaaagc gacaagtatg taaatgccat taattttcaa
     6481 ttcatagttg cagaaaacaa agcgctcctt cactatcatg aaggactgaa gaacgaagta
     6541 tttattgaag gaatctatcg caaagcattg caagctaaat cgctctccta catatatttc
     6601 atgggagaca atatgaattc gctagagcca ttcgccaacg agatatttat gcccattgaa
     6661 aaagatgtgt actacgtatc aggtgaccaa tttggaacag acacccgtct cgattgcagt
     6721 tccgttaagc ttaccaccaa caaagatgca agcggaaata taattcgcga ctatttgatc
     6781 ataggtaaag acaaaaaaag agttgaagaa ttgaataaca atcctgaatt acttgaaaaa
     6841 ctattaataa agagttgcat tgaaatgtgt gaagcctaca atgtggttcg agccggaaga
     6901 aatccaatgc cgggtgaagg tcgtgttgat gaaaaacttg caagcgaggc ttttgagtta
     6961 accaagaaac atgctgcaag taaaaactgg ccacaaaagc ttgaatactg ctatatcaag
     7021 agccctgaat gggtaatcat ccgtaataaa gtgacaggag taattctttc ccgctctacc
     7081 agagtagttg ttataatgac ttccaatgga aagtgtatgt gggaagagat ggaaattcgt
     7141 caggattatg acggtgctca attcggcaaa acctatttct atgggaattc agggaatata
     7201 gttcccgcag aatgtacaga tgcaatgaaa aagaaaaaat aacatatcat ttccttcagg
     7261 aaaaaggctg tttcaattgg aaacagcctt tttttattga attatttctc ctgaaaacgc
     7321 ttccaggacc tcatctaaaa cagcatacac atcggcaggc tgcaacattg ttacaaggcg
     7381 agatctgtat tctttaaaat ggggaatgtt acggaagtaa tttgcatagt gtctccgcat
     7441 ctcaacaatt ccctcgcgtt cacctttcca acgaatggaa aactccaaat gctctcgcgc
     7501 tgcttcaaca cgctcgatca atgatggtgg cgccatcgtt tctcctgttt ccatgaaatg
     7561 cttcacttca cgaaatatcc agggataacc aatactcgcc cttccaatca taattccgtc
     7621 tacgccgtaa cggtttttca tttcgagtgc tttcgcaggt gaatccacat cgccatttcc
     7681 gaaaatcgga atttttattc gcggattttc cttgacctga ccaataagcg tccagtcagc
     7741 ctctccctta tacatctgca cacgcgttcg accgtgaatg ctcaatgcct gcacaccaat
     7801 gtcctgaagg cgttcggcaa cctccaaaat atttttggta tcatcgtccc accccaaccg
     7861 cgttttaacg gttacaggca atttaacagc ctttaccaca gcctcggtaa ggcgcatcat
     7921 tttcggaatg tttttcagga ttccggcacc tgcatcttta catactactt tttttaccgg
     7981 acaaccataa ttgatatcga taagttccgg tcctgccgat tcacaaattt gcgcagcctg
     8041 aaccattgaa ctttcttctc cgccaaaaat ctggattcct atggggcgtt cataatcgaa
     8101 cacatccagt tttttgcggc tcttccatgc atcgcgaatt agtccttcgg atgaaatgaa
     8161 ctcagtatac atcaaatcgg caccatactt tttgcaaagc ctgcggaaag gcggatcact
     8221 gacatcctcc attggagcca atagcaaggg aaacgctccc aaatcgatat ttcctatctt
     8281 taccacttct gcaaaaatac gcaaaccatc gccatcatga aaacaccaaa tatccgtcac
     8341 ctcaacccta tgttgcagat gtttgtgctt ttcggactca ttttcattac cctttttctg
     8401 gtcatgttgc tgggtaaatt agttgctgat tggggatatg gtatcaatct tctcgatccg
     8461 gattttgcca aagacccctc tgccgaaggc gttactgctg tacaacgctg gctaatggta
     8521 ttgaatcagc tcggattttt tctcatacca gccctgctgt tcgggcaaat gatgtattcg
     8581 gaagagcaat cctacttccc gctcaaaaaa ccaaacctga tgcaattcgg actcgcaatc
     8641 gtattggtat ttgcaatgca gactccaatt gattttttac tccgattaaa ttcatcatta
     8701 cctctgggcg atgaggtgat gagtgatatt aatgagatgg aagtaaaagc cgcgttcatg
     8761 ataaaacaac tgataggaga aggaagtttt gccgatgttg cgatagcaat gattattctc
     8821 gcaattattc cggccatcgc agaggaattt ttctttcgcg gtgtactact tcaaactttc
     8881 cgggcatcct tccgcaacgc tcatctggca gtatggatga ccgcaatagt tttcagtttt
     8941 attcatatgc aatttcttgg attcattcca agaatgatgc tcggcgcatt actgggttat
     9001 ttgatggtat ggagcggaag tatctggctt tctataatcg ttcacttttt caataatgcc
     9061 ctgggagtat ttctaatgtt tcaatttggt gcagaagtaa atacaatctc ccaacaagca
     9121 gagaccaatt ggctaatgct ttctgtgggt agcttattta ccatcggtac gatctatttt
     9181 ttccatcgct tccgacttaa ggatcaaaac catcaagaac ccttcaatac atttgaatag
     9241 caagcggctc acgctctatc aaccgttgcg gcacaataga tcggattact ttcattcgtt
     9301 ctttggataa agcatctttc tgatccaaat caacatacgc aacaccatac aatttcatac
     9361 tccactcatc gcgaagtgaa aggatacatt gctcaattgc atcctgaaca gcaatgtaaa
     9421 acgcatagga cgttgcattg tcattttgca gggagatcac tgcatcttta caaataaaat
     9481 atcctgcgcc ggtttgttga accatagcga gacgatcctt ccagaattct tcttcgtgtt
     9541 tacggctatc atccgctttt atttctgacc aattctgcag gcaaatttct tcatttacaa
     9601 aatccatttc tgcaagactc ctttcccttt taggattggt caggaagtcg cgtaacacag
     9661 ccttcaattc cggaatggtt gtaattttat cttccaccag taattgattg gcagcattga
     9721 tgcgaatatc cagaacgttt cgctcgtgga tttccttttc aatttctgtc tccacctttt
     9781 gaggcagtct tcgagctatt ccttcttcgg tttccattgt agttaccagc aaaaaaaaca
     9841 ccagtaaaag aaaagcaata tcagcagttg agccggcatt gatctcagag tttcggttgg
     9901 tcttcatcgt tattggttta caatagaaac ttcaaacaaa aacttatagt gtatattcct
     9961 tcactcaatg aaccaatggc tgcaaaccaa aatctaacgg taaaaaaaag cccatcttag
    10021 tggatgggct tttaatttca aaacaaaaaa caagattaag gctgaacgcg ttttcttgtt
    10081 gtggaatagt taatctttaa cgcatcaacc tgacgcaatt cgtgcttcac acgagaaacg
    10141 atacccatac gggtcttttc atcaactttt agagatgtag ttacgaaagg aatatccgtt
    10201 tcattacgct ctgtgcgttt gcggatcacg aatggtccaa catcagttgc ctgagttgca
    10261 atctgatcat cgagctgaat gcgaggctcg gttccataca tgctgcggta acgcattgtt
    10321 gccggcccga tgtagatgta atctaccaat gattttttct caagcttggt gattgcttct
    10381 gcttcaggtt ttttgatttc aattttcaga tccacttcac gcataactgt tgttaccatg
    10441 aagaaaaaaa gaagcatgaa tacgatatca ggcaaagaag ccgttgaaat ggcgggtgat
    10501 cctgaactat cgtctttttt aaattttgac atacgtttcc ttttgtagta ttaatacgca
    10561 gggatgtttt tgggctcttg ttcagcaata cgctgcggaa caatagcacg aatcaccttg
    10621 atcttttctt tatccgagtc gttggaggag ttccaatcgg taaaattctc tccatatacc
    10681 ttttgcgacc attcattacg cagttcgtta atggcagatt gcatctgatc ctgaacttca
    10741 acataaccct tgtacgaggt tccattatcg ttacgaagag aaatgatggc gagtttaggc
    10801 agtgtacgga aggggcctac tttttcagcg gcagcaagtt tatcctccca atcggaaagt
    10861 tctgacttga ttacccctaa tgaagcagca ggtgtctttg gattatcgat cagttgtttg
    10921 cgatttgcaa tttcctggcg cgctctttca acagtgattt cttccattag agggagattc
    10981 tcactattat aagggttcgt aagaaactct ctgatcttcc ctttgagttc accgatttgg
    11041 atgtaatcgc tctcaaccag gagctggtca ttcgaattca aattcacttc gaaaacatcg
    11101 cgctcgttga tctcgacttc cattttataa tccggtggaa gtttttgcgg cagtgagcgc
    11161 acaatacctt catcggtatc catggtagta gttaccagaa agaagattaa caacaggaag
    11221 gcgatgtctg ccatcgatcc tgcattgatc tcttccaatt ttctcttagc cataacttat
    11281 aagtttaagc tgaaaaacgc tttaccagac cccaaaccca accggccgca ataagaacta
    11341 ctgccagcag tccgaaaatt gaaaccagtt tgatagagaa atcagaaaac ttaacgaacc
    11401 cgggattaaa caattcgggg tctcggtatt tccatgattc aatcattgcg tcggaagcag
    11461 ttacccaact gaacaagacg attgctccaa aaatggcaat tccaacaaga aagggaatgt
    11521 tgtttctcaa gtcaatgaac gtcaggtaaa cagcaaacag aatcgcaaaa atcaaccccc
    11581 cgtaaatcat atatatcgtg agattcatgg taaatgtcag cttaccgttg aaggagatgt
    11641 ttgagttgat cgcatcaata tgactattga ttgccagctt ttcattctcg tcagcagcac
    11701 cggcaagctt tccttcccag ataccgacat tttccttgag tgttttttct tcatcagcaa
    11761 ggtgaccggg tcccagcaca gcggctgttc cctgcagcat cagaagaata ccaaaaacaa
    11821 agcccagtac cattagtacg cctttgaaaa tggtaagtat tgagtgcaac ttagtgtctt
    11881 tcatacagat cgtttttgat cgttaatagt tgcccgaaat tacttaccgg gaaatacctt
    11941 gtgcttaaga aggatatcca tgatagaaat ggaagagttt tccatatcaa ttaccaggtt
    12001 atcgatcttc gacaataagt agttgtagaa taactgaagg atcatcgcaa cgatcaaacc
    12061 acctactgtg gtaagaagtg ccaccttaat accatccgca acgatctggg gagagatgtt
    12121 acccgcacga gcgatgtcat caaaagcctg gatcataccg ataacagtac caaggaatcc
    12181 gagcataggt gcaagagcga taaagagaga aagccaggta agaccgtttt ccatctgacc
    12241 attctgtacg ctaccgtagc tgatgattgc cttttcaacg ttatcaggac cttcatccac
    12301 acgcatcaat gcctggtaag cgatagaagc aacaggacca cgtgtattgc ggcaaatttc
    12361 ctttgcagct tcaactccac ctgatttaag tgcagcttca accttttcga ggaacttctc
    12421 agagttacct gtagccatgt taaggtaaat aatacgttcg atgctgatcg caagaccaag
    12481 gatcaaacaa attaaaacca aggacatgaa gcggggatcc ccttcaatga acttttcttt
    12541 tagcacctga gtgaaagtaa ctttcggagc ttcatcagca ttttcacctg aagctgtagc
    12601 ctcagtaggt tctgccggtg tttcagcagg agttgtagtt tcagtcgctg aagtagaatc
    12661 tgtattctgc gcttttgcgt actgagtggc tccaatagta aggaggccgg aaattgcgat
    12721 caatgaaagg atctttttca tgtggaaccg atttgttttg agaacttgta tttctgggtt
    12781 tttagtatga actgaaaatt gtgggtgtag caacggttgt ttggaaaatg taagcggata
    12841 aaattttaag attcaatctg cattcacttt ccacagacta acaaaataat ctgtaccgct
    12901 tcctacggta atttatgcgg agagagaggg attcgaaccc tcgataccct tttg
//
LOCUS       DAOMYG010000023        12613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1672_length_12613_cov_11.252986, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000023 DAOMYG010000000
VERSION     DAOMYG010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 12613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12613
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1672_length_12613_cov_11.252986"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PK610_01510"
     CDS             complement(<1..231)
                     /locus_tag="PK610_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRJ34628.1"
                     /translation="MSLGFDFAQVATATMVLFAVIDILGSVPLIIEIRKKAGEIFPMK
                     ATMVSLLIMVAFLFLGESILKIIGINVNAFAVA"
     gene            complement(228..800)
                     /locus_tag="PK610_01515"
     CDS             complement(228..800)
                     /locus_tag="PK610_01515"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRJ34629.1"
                     /translation="MKNLAIFASGTGSNAERLMSYFRDSGYAVVKLVVCNNPSAGVLE
                     IARRNGVEVLLQDNSIFSGDGASLVDELKHRNIDLIVLAGFLRKIPVSLIHSFSDRII
                     NIHPALLPKFGGKGMFGDNVHQAVLAAHEKETGITIHLVNEEYDKGRILFQGKCMLHE
                     NDTVADVRAKVHELEHRFFPEEVERYIKGI"
     gene            987..1682
                     /locus_tag="PK610_01520"
     CDS             987..1682
                     /locus_tag="PK610_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007844577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HRJ34630.1"
                     /translation="MVKSFLSKQSIPAGEEIKGTAIRDSVFAFIERHYEGFGQEDYLS
                     LDELNKIRRDFLSELIEQEKGELVIIDKEVLSAIKNNSILSENILEETEKEITRGERI
                     ADKVASFGGSWTFIIIFFSFLIIWMAINVWILSEKVFDPYPFILLNLILSCLAAIQAP
                     IIMMSQNRQEQKDRLRAEHDYKINLKAELEIKLINEKIDHLLIHQNQKLLEIQAIQAD
                     YLDDLIKEIKKHK"
     gene            1939..2517
                     /locus_tag="PK610_01525"
     CDS             1939..2517
                     /locus_tag="PK610_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRJ34631.1"
                     /translation="MDQRRSVRHFSAAPVPRAVIENLILTASSAPSGAHKQPWTFVAI
                     SNTELKHAIRELAEKEEKESYDGRMSEEWLKDLAPLGTDWQKEFIDIAPWIIVVFKRP
                     YEIVNGEKKNNYYVTESVGIASGFLLAAIHMAGLVSLTHTPSPMNFLVKALERPENER
                     PFLLIPVGYPADDAMVPDIHRRKLEEVAVFVK"
     gene            2612..3007
                     /locus_tag="PK610_01530"
     CDS             2612..3007
                     /locus_tag="PK610_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34632.1"
                     /translation="MLKCLTIVFGLMIPLLSTAKGGKGFYCVSGVARSTENGIIKNSS
                     FKITHGAKESNGVTDSNGKFRILVEWQTACPSGISRDAWNNENARLNPEYIYITFQGI
                     TLRIENEWEKYAGRGRSPKKVTRKLNLEF"
     gene            3404..6958
                     /locus_tag="PK610_01535"
     CDS             3404..6958
                     /locus_tag="PK610_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ34633.1"
                     /translation="MKTILLHFISRFFVIMSLVLISKDAYSTHAQSADITYTCVGGNT
                     YNVRLAFYRDCAGVAAPNTVSINVASVSCGQNFNVNMNRIPNTGIDVTPICPTMTSQC
                     AGGNYPGVQEWVYQGNITLPAQCIDWVLSFNLCCRNNAISTINNPGGDNIYVEARINN
                     LMFTCNNSPQFSNRPVPFICVSQNYCFNHGAIDSDGDSLVYQLIPPMTGPNTSVNYFP
                     GYSAAQPLMSVPATTINSATGDICMTPTMLEVGVMAVRVSEYRNGEFVGSVIRDIQVR
                     VIPCTNSNPSITGINGGNNFSASVCAGQTLNFNILSSDPDAAQNLSLSWNNAIAGASF
                     TTNIAQHPTGTFSWTPNSTMISSIPYCFTVTVTDDNCPLLGSQTYSFCITVTGFNITA
                     SSTPANCGAANGTASVTVNGGNAPYSYSWSSGNNTASVNGLQAGNYTVNVSDATGCIM
                     SANTLVAQGAAPGNIQLSAQNVSCFGGNDGAATVNANGGQQPYTYQWSNGGTSATITG
                     LTTGLYSVSVTTANGCTTNGTINVPQPSAPLSLNITSTSVLCNGQNNGSATVNVNGGT
                     GPYNYAWNTNPQQSTATANGLIAGQYTINIMDAEGCTQSGIAIVAQPAAISIQTTSTT
                     PVSCNGGANGSATVLANGGNGTISYQWNNGTTGTTINSVAAGNYTVTASDMSGCQQTH
                     SVTINQPLPLSATIVSQNDITCFGANNGSIQTTVNGGTAPYNYQWNINPPQNSASINN
                     LAPGNYFMNVLDAQGCTTSLTTTISQPAPLTLTVSPGDTICPGTITTISATANGGSGG
                     YVYHWNNNLSNATSHNISPAASTTYSVYASDANACTTTAQTIFVHVNDINLLQLGVTA
                     TPDLCIGQTGLVFTEMGTATGIYIITWNNNLPNGPGPFSITPTTSGYYTATITDGCGN
                     QRVESTWVEVHPNPDVQLSPQLTNACGSAPLTFFNDMPNENGSSFAWNFGDGNTSTLE
                     VVTHPYTVSGVYNVSLIVTSPWGCVGTDQTQVGVTVRLQSEADFEISDLDLSIFHPEA
                     RFTNTSTNATFASWMFGDGNGSTVYSPMHTYANTGTYEVTLISNNNQNCPDTITKFIQ
                     VSPEFTFYIPNAFTPDGDGKNDVFYGKGENIAEYDLMIFNRWGEMVFNTQNINTAWDG
                     TYRGSEEPKADVYVYKVKIKDSVRGEYHFYDGHVAIVK"
     gene            7054..9894
                     /locus_tag="PK610_01540"
     CDS             7054..9894
                     /locus_tag="PK610_01540"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRJ34634.1"
                     /translation="MTRILLLLFVAMPFTAGAQRKSTIENSYTLARERMSAWEKHKSK
                     SDQSLLKGIEFRSVGPSVMSGRVVDIEVDPNDETHFFVAYATGGLWETRNNGTTFTPL
                     FDKEWIFGIGDIAIDWKSDVIYVGTGENNSSRSSYAGIGLFRSSDHGKSWQHLGLEET
                     HHIGRVILHPTDANTIWVSSIGHLFTPNKERGLYKSSDGGKTWKQTLFVNENTGVIDL
                     IVDEKNPSVLYAASWHRERRAWNFIESGNGSGIYKSTNGGDSWQKISTPESGFPQRDG
                     VGRIGIALCKNKPEIIYAFLDNQALRKDFKKESDPEALSKDQLKKMSKEEFLSLDEKK
                     INRFLQENNFPKKITVELVRTKILDNSISPKTLAEYLEDANALLFDTPVTGAEFYRSN
                     DGGKTWKKTHDTYVEDLVFTYGYYFGQISIDENDPEKIYASAFVVIKSEDGGKTFESI
                     NGDNVHVDHHALYVNPKRKGHLINGNDGGVNISYDNGTSWIRCNTPAVGQFYTINVDM
                     ASPYNIYGGLQDNGVWYGSSQHLESLSWQMEGEYPWKTILGGDGMQVMIDPRDNSTVY
                     TGYQFGHYYRINKNTGESFYITPKHELGERPLRWNWQTPIWLSTHSADVIYMGANKVF
                     RSLKKGEEFVAISGDLTKGGKPGDVAFGTITALHESPLKFGLLYAGTDDGFVWLTKDG
                     GNSWNNITGTLPADFTVRRVQASRFVEGRVYVCLNGHTRDDFSTMVFSSEDYGKTWSR
                     IATDISGEAVNVIREDPANENLIYVGTDHGVYASLDKGKSFQLLNTNFPNVPVHDLVI
                     HPRDKELVVGTHGRSVYVANIAPLQELTTVVQSQSLYLYTPKEITHSRMWGKSWSKWE
                     KGYEPEIAIYFWTTAAGSANIELREASGKLVYADKTQHQKGLTRFMYNLKNNQEKTEN
                     GKNEFLKPGDYKLSVSSGASTSTTTLKITENR"
     gene            complement(9951..12095)
                     /locus_tag="PK610_01545"
     CDS             complement(9951..12095)
                     /locus_tag="PK610_01545"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ34635.1"
                     /translation="MSRALFLFIVMICLHSGNVYSNADSLWSVYNNRAEPDSVRLKAM
                     DELSWPLVFSVPDSAISLCNFSIGEFAPKGASKGLGQILRNKAVAFIMKGEYNSALDA
                     YQQSEQNCLGAGDLDGLASTYNGYGIVFSRIGQHARSIEYYYKALSCFERLSDKQGMS
                     IAYNNIGLIHTKRQEYDKALALYDKVLAIQKELGNQKGINTALANMAEVYLKTEQFDK
                     SIEIYEQALEGAQRMNDEFMVATVRNNMGGAYADKGDYDKALYHFFYSLPIRNRAQDV
                     QGQIDTYNSIAQVYVKQKDFKKALEFARRSYDLSATVGSPEGIKNSAFTLFNVFEGQR
                     NADSALKYYREFVTIKDSLYNSENTAKLVEEEMRYGFDQREREMMLEQQRRDIVHEES
                     NKRQRLQKIYMGIGLALCMLILLVVWRGLQARKRANRIISEQKELVEEKNKEILDSIR
                     YAQHIQEAILPAKEFMDGFLPEYFIYYLPKDIVSGDFYWAEQFGDLSLFAAVDCTGHG
                     VPGAFVSIVANNNLNRCVKEFGLREPAAILDKLNLLVNETLRQRRDVSSVKDGMDIAL
                     CSIRRLPDGSVELQYAGANNSMFLFAAKGSLPEKHLAIEDPESSFAVFEIKPDRQPVG
                     NFLEHYSEPFTKHKLKLQKGESLYILTDGFADQFGGPNGKKYKQSQFKKLIFQNGHLS
                     MQEQRDVLHTAFHTWKGDMEQIDDVCVIGVRV"
     gene            complement(12237..>12613)
                     /locus_tag="PK610_01550"
     CDS             complement(12237..>12613)
                     /locus_tag="PK610_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006264544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRJ34636.1"
                     /translation="GEHVGMAFQIKDDIFDFGSGEDIGKPTGIDIKEKKMTLPLIYAL
                     NNAEKGVRRKIINYVKNHNEDPKKVQEVVDFVLKSGGVQYATKRMHEFKDKALTILHT
                     FPESEYRRALEGLVLYTVERKK"
BASE COUNT         3919 a         2783 c         2513 g         3398 t
ORIGIN      
        1 tgctactgca aatgcgttta cgttaatgcc aataattttt aggatagatt cgccaaggaa
       61 aagaaaagca accatgatca gcaatgaaac cattgttgct ttcattggaa aaatctctcc
      121 tgcctttttt cgaatctcaa tgatcaacgg aactgatccc agaatatcaa ttaccgcgaa
      181 taaaaccata gttgcggtag ccacctgtgc aaaatcgaaa cctaaactca tatgcctttg
      241 atatagcgtt caacttcttc aggaaaaaaa cggtgttcca gttcatgcac tttagcgcgc
      301 acatcggcaa cagtatcatt ttcatgcagc atacatttgc cctgaaaaag aattcgtcct
      361 ttatcgtatt cctcattcac aagatgaata gtaattccag tttctttttc gtgtgcagcg
      421 agaaccgctt ggtgaacatt atctccgaac atgccctttc cgccaaactt aggaaggagt
      481 gcggggtgaa tattgataat acgatcagaa aatgagtgaa tgagtgaaac aggaattttt
      541 cgcaaaaacc ctgcaagtac aatcagatca atatttcggt gctttaattc atcaacaagg
      601 gaagcaccgt ctccggaaaa aatactattg tcctgcaata gaacttccac cccgttgcgt
      661 ctggcaatct ccaacactcc ggcagaagga ttgttacaaa ccaccaattt aacaacagca
      721 tatccggagt cgcggaaata ggacatcaga cgctcggcat ttgagccagt tccggaagca
      781 aatatggcaa ggtttttcat tagagggaca aaggtagaga ataaggcaat attgataaca
      841 gattgaggga aaaactatta ctcaatcatg aaaataagaa attccaccgg caatcttgct
      901 gccacaagtt caaaatgttt aggtatttga aaacccatta ccattgcttt ttgtaacttt
      961 aagttttacc tcttatccaa atttcaatgg tcaaaagttt tctcagcaaa caatctattc
     1021 ctgcgggtga agagataaaa ggaaccgcca ttcgtgattc tgtatttgcg ttcattgagc
     1081 gacattacga agggtttggg caagaagatt atctctcgct ggatgagtta aataagatta
     1141 gacgagattt tctttctgaa ttgattgaac aggaaaaagg cgaattggtc ataattgaca
     1201 aggaagtttt aagtgcgata aaaaacaatt caatacttag tgaaaacatt cttgaagaga
     1261 cggaaaagga aataacccgg ggggagcgaa ttgccgataa ggtagccagt ttcggtggga
     1321 gttggacctt cattataata ttcttttcgt tccttataat atggatggca atcaatgttt
     1381 ggatactgag cgaaaaagta ttcgacccct atcctttcat tttgctaaac ctcattttat
     1441 catgtttagc agctatacag gcacctatta ttatgatgag ccaaaatcgt caggaacaaa
     1501 aagaccgtct ccgtgctgag cacgattata aaatcaactt aaaagccgaa ttggaaataa
     1561 aactgatcaa tgaaaaaatc gaccacctgc ttattcatca aaatcaaaaa ttgttggaga
     1621 tacaggccat tcaggcagat tacctcgacg atctgatcaa agaaataaaa aagcataaat
     1681 gaaaatcata ctttttattt tcaaacatta gatcattaca tttattttcc aaaaacctgc
     1741 ttcttgttat tctttgaatt ctctgacaat actataagtg atctcttatt aactaagagc
     1801 ttcgctttct caaaataaat atttgatttt tttatctgaa acagcttctt atttatggaa
     1861 ttccccttta tcccctatag ccgcccgatc ctcgcggaga aagaactcat tcgacgctcg
     1921 gaagaattct tcgaacaaat ggaccaacgt agaagcgtac gacatttttc ggctgctccc
     1981 gttccgagag cagtcatcga gaatctgatt cttaccgctt cttccgctcc aagcggtgcg
     2041 cataaacagc cctggacatt tgttgctatt tccaatacag aactgaaaca tgctatccga
     2101 gagttggcag aaaaagaaga aaaggaaagt tatgatggtc gtatgagcga agagtggtta
     2161 aaagaccttg ctcctcttgg aacagattgg caaaaggaat tcatcgatat agcgccttgg
     2221 atcattgttg tgtttaaacg tccctacgaa atagtaaacg gagaaaagaa aaacaattat
     2281 tacgtaaccg aatctgttgg aattgcaagt ggatttttgc tggcagctat acatatggcc
     2341 ggactagtct ctcttacgca caccccttct ccaatgaact ttcttgtcaa agcacttgaa
     2401 cgtcccgaaa atgaacgtcc ctttttatta attccggttg gatatcccgc tgatgatgca
     2461 atggttcccg acatccatcg cagaaaactg gaagaggtag ctgtgtttgt taagtaattc
     2521 tgaatgacct aatttctaat gtagaaaagt aaattatcgc gatattttct tttcaaatcc
     2581 atttagtata ataaataaaa tttgattcta aatgttaaaa tgtttaacta tagttttcgg
     2641 acttatgatt cctctgttgt caaccgctaa aggagggaag ggattttatt gcgttagtgg
     2701 ggttgcccga agtacagaaa acggtattat caaaaacagc tcatttaaaa tcacccatgg
     2761 agcaaaggaa tcaaatggtg ttaccgattc caatggcaaa ttccgtattc ttgttgagtg
     2821 gcaaaccgct tgtccttccg gcatctcacg agatgcatgg aacaatgaaa atgcaagatt
     2881 aaaccctgag tatatctaca tcacctttca gggaatcaca ctccgcatcg aaaatgagtg
     2941 ggagaaatat gcaggccgag gccgatcccc taaaaaggtt acccgaaaac tgaatcttga
     3001 attctaatcc gagattcttt atcccgcctc tttaattatc atcagacagg ttattgtatt
     3061 acaactacgt ttcaataatt cagtagtctc ttatatcttt tcaaaaggct atttctatcc
     3121 caccactcct aaaaccaatt aggtcgaaaa ccccttgttt ttcaatgctt ttcaaacgtg
     3181 gtatgcgcgc atactattga caattttctt ccaaagaata ttctgttgaa aacacagggt
     3241 tgcagaataa tgttctgcat gcatacgatt atccgaaaca aatgcgacaa tgtgacgaaa
     3301 gtgagggaac ggcctgacaa ccctgggcaa cggtagtgcg ttttaagaac aagaagcaag
     3361 ttctttaaat cattcaccat aaaaaagaaa ggaacggtgc gttatgaaaa caattctcct
     3421 ccatttcatt tcgcgattct ttgtaataat gagcctggtg ctcatcagca aagacgccta
     3481 ctcaactcac gcacaaagtg ccgacatcac ctatacctgt gtcggcggga acacctacaa
     3541 tgtgcgatta gccttctacc gcgactgcgc gggcgtggca gctcctaaca cggtaagcat
     3601 taacgttgca tcggtatcgt gcggacaaaa cttcaatgtg aacatgaacc gcattccaaa
     3661 cactggaatt gatgttacac caatttgtcc gaccatgacc tctcagtgcg ccggcggaaa
     3721 ttatccgggc gtgcaggaat gggtttatca gggaaatatt actttaccag cgcagtgcat
     3781 tgattgggtg ctcagcttta atctctgttg cagaaacaat gccatttcta ccattaataa
     3841 cccggggggt gataatattt atgtagaggc tcgcatcaac aacctgatgt ttacctgtaa
     3901 caactcaccg caattctcga atcgtccggt accatttatc tgtgtgtcgc aaaactattg
     3961 cttcaatcat ggtgcaatcg attcagacgg cgactctctg gtataccagt tgattcctcc
     4021 aatgacaggg cctaacacat cagtaaatta tttccccgga tattctgcag cacaaccttt
     4081 aatgagcgtt cctgcaacaa ctattaattc agcaaccgga gatatctgta tgacaccaac
     4141 catgcttgaa gttggagtaa tggccgtaag agtttccgaa taccgcaacg gtgagtttgt
     4201 tggaagtgtg atccgtgata ttcaggtgcg cgttattccc tgcacgaatt caaatccatc
     4261 catcacagga attaacggcg gaaataattt cagtgcaagc gtatgtgcag gacaaacact
     4321 caacttcaat attctttcca gtgatcccga tgcagcacaa aacctcagcc tcagctggaa
     4381 caacgctata gcaggcgcat cctttacaac gaatattgcg caacacccaa cgggcacatt
     4441 cagctggaca ccaaactcta ccatgatctc atcaattcct tattgcttca cagtaacagt
     4501 gaccgatgat aattgtccgc tcctgggttc acaaacttat tcgttctgta taacagttac
     4561 aggattcaat attacagcaa gcagtacacc agcaaactgt ggtgccgcaa acggtacagc
     4621 atcagtcacc gtaaacggtg gtaatgcgcc ctacagttac agttggtcga gtggaaataa
     4681 taccgcaagt gtaaacggat tacaggcagg aaactatact gtaaatgtaa gcgacgcaac
     4741 aggttgtatt atgtcggcca atacacttgt tgcacaaggc gctgctcccg gcaatattca
     4801 gctatctgca caaaacgttt cctgcttcgg tggtaacgat ggtgctgcta ctgtaaatgc
     4861 gaatggtggt cagcaacctt acacgtatca atggtcgaat ggcggaacaa gcgctacaat
     4921 aacaggatta actacaggct tgtacagtgt gagcgtaacc actgcaaatg gttgtaccac
     4981 caacggaact atcaatgttc cccaaccttc tgcacctctc tctttaaaca ttacaagtac
     5041 aagtgtgctt tgtaacgggc aaaacaatgg ttctgcaacc gtaaatgtaa atggaggaac
     5101 ggggccgtac aactatgcat ggaacactaa tcctcagcaa agcactgcaa ctgcaaacgg
     5161 attaattgca ggtcaataca ccatcaacat tatggatgcg gaaggttgta cacaaagcgg
     5221 aattgcaatt gtagcacaac cggcagcaat cagtatacaa accacctcta ccactcctgt
     5281 atcatgcaat ggtggcgcaa acggatctgc aactgtgctt gctaacggag gaaacggaac
     5341 aatctcttac caatggaaca acggcacaac aggaacaacg atcaatagcg ttgccgctgg
     5401 aaattatact gtaaccgcaa gcgatatgag tggttgtcag caaacccatt ctgtaaccat
     5461 taatcagcca cttccactaa gcgcaaccat tgtttcacaa aacgacatta cctgttttgg
     5521 tgcaaacaat ggatccattc aaacaacagt aaatggtgga acagctccct acaattacca
     5581 atggaacatt aacccgccac aaaacagtgc aagcataaat aatctcgctc ccggaaacta
     5641 cttcatgaat gttcttgacg cacaaggctg cacaacatct ctcacaacca caatttcaca
     5701 acctgctcca cttacgctca cagttagtcc gggagacacc atttgtccag gcactattac
     5761 tactatcagt gccacggcaa acggaggttc gggtggatat gtttatcatt ggaacaataa
     5821 tctttccaac gcaacatcac acaacatatc tccagcagcc agcacgacat attctgtata
     5881 tgcaagtgat gcaaatgcat gtacaacaac tgcacaaacg atttttgtcc atgttaacga
     5941 tatcaatctg ctgcaattgg gtgtaacagc aactccggat ctttgtattg gtcaaacagg
     6001 attggtattt accgaaatgg gaaccgcgac aggaatctat atcattacct ggaacaacaa
     6061 tctgcccaat ggtcccggac cattctcgat aacacctaca acaagcggat attacaccgc
     6121 aacaattact gatggatgtg gcaatcaacg cgtggagtcc acctgggtag aagtacatcc
     6181 caatcccgac gtacaactca gcccgcaact gaccaatgcc tgcggctccg ctcccctaac
     6241 attcttcaac gatatgccca atgaaaacgg atcatcgttc gcatggaatt tcggagatgg
     6301 aaatacatcc actcttgagg ttgttaccca tccgtacaca gtgagcggag tatataacgt
     6361 atcgcttatt gttacatcac catggggatg tgtaggaaca gaccagacac aagtgggagt
     6421 taccgttcga ctgcaatcgg aagccgactt tgaaatcagc gatcttgatc tctccatttt
     6481 tcatccggaa gcacgattca caaacaccag caccaatgca acatttgcaa gctggatgtt
     6541 tggcgacgga aacggctcga cagtatactc acccatgcac acgtatgcta acacgggcac
     6601 ttatgaggtg acattgatat ccaataacaa tcagaattgt ccggatacca tcaccaaatt
     6661 catccaggtt agtccggaat ttaccttcta cattcccaat gcattcactc cggatggaga
     6721 tggcaagaat gatgtcttct atggaaaagg tgaaaatatt gcagaatacg atctgatgat
     6781 ctttaaccgc tggggtgaga tggtgtttaa cacgcaaaac atcaacactg catgggacgg
     6841 aacatatcgc ggatcagaag agccaaaagc cgatgtgtat gtttacaaag taaagatcaa
     6901 agacagcgtc agaggagaat accacttcta tgacggacac gtggcgattg tgaagtgaat
     6961 ttagaacacc aataaacaaa gctccctaag gcccactgag atttttcttg gtgggccttg
     7021 gtgttgttag tggcttagtg gttatatttg aacatgacac gaatcttact cctcttattt
     7081 gttgcgatgc catttactgc aggcgcgcaa cgtaaatcca ctatagaaaa ttcctatacg
     7141 cttgcaagag aacgcatgag cgcatgggaa aaacataaaa gcaaaagcga tcaatcactg
     7201 ctgaaaggaa ttgaattcag atcagttggt ccgagtgtaa tgagcggacg agtggtagat
     7261 attgaagtcg accccaacga tgaaacccat ttttttgttg cttatgccac aggaggatta
     7321 tgggaaacaa gaaacaatgg aacaacattt acccctcttt tcgacaagga atggattttt
     7381 ggaatcggtg atattgccat cgactggaaa agcgatgtga tctatgtagg aacaggagaa
     7441 aacaactcca gtcgttcttc ctacgcaggt atcggattat ttcgtagcag cgatcatgga
     7501 aaaagctggc aacatcttgg actcgaagaa acgcatcata tcggtcgggt aatacttcac
     7561 cctacagatg caaataccat ttgggtgagc agtatcggtc atcttttcac tcccaacaaa
     7621 gaacgtggat tgtataaatc aagcgacggt ggaaaaacct ggaaacaaac cttgttcgtg
     7681 aatgaaaata ccggggtaat tgatcttatt gtagatgaga aaaatccatc tgtgctttac
     7741 gccgcatcgt ggcatcgcga gcgccgtgcc tggaatttta ttgaaagcgg aaacggttca
     7801 ggaatataca aaagcacaaa tggcggtgac agctggcaaa agatttccac tcccgagagc
     7861 ggatttccac agagagacgg cgtgggaaga atcggaattg cgctttgtaa aaacaaaccc
     7921 gaaattatat atgcttttct cgataatcag gcattacgca aagactttaa aaaggaaagt
     7981 gatccggagg cattaagtaa agatcaactg aaaaaaatga gcaaagaaga atttctgtcg
     8041 ctcgacgaaa aaaagataaa tcgcttttta caagaaaaca attttcctaa aaaaataact
     8101 gttgagctcg ttcgtactaa aattcttgac aacagtatca gtcccaaaac gcttgcagaa
     8161 taccttgaag atgccaatgc acttttgttc gacacccctg taaccggggc agaattttac
     8221 cgatcaaatg acggagggaa gacctggaag aaaacacatg atacctatgt ggaagatctt
     8281 gttttcacct atggatatta ttttggtcag atcagcatag atgaaaacga tcctgaaaaa
     8341 atatatgcat ccgcttttgt tgtaattaaa tctgaagacg gaggaaaaac atttgaaagc
     8401 atcaacggcg acaatgtgca tgtcgatcat cacgctttgt acgtaaatcc aaaacgaaaa
     8461 ggtcatttga taaacggtaa cgatggggga gtaaatatca gctacgacaa tggtacaagc
     8521 tggattcgtt gcaatactcc tgcagttgga caattctata ccataaatgt tgacatggca
     8581 tctccctaca acatttacgg agggttacag gacaatgggg tatggtatgg atcatcgcaa
     8641 cacctggaga gtttgtcctg gcaaatggaa ggtgaatatc cctggaaaac aattcttggt
     8701 ggtgacggca tgcaagtgat gatagatcca cgcgacaaca gtacagtgta taccgggtat
     8761 caatttggac attattaccg catcaataaa aacacaggcg aaagttttta tataacaccc
     8821 aagcatgaac tgggtgagcg tccattgcgc tggaactggc aaacacccat atggctatct
     8881 acacacagtg cggatgtaat ttatatggga gcaaacaaag ttttccgttc gcttaaaaaa
     8941 ggagaagaat ttgttgccat ttcaggagac ctcaccaaag gcggcaaacc cggtgatgta
     9001 gcattcggta caattactgc gctgcacgaa tcgcctttaa aatttggact gttgtatgcc
     9061 ggaacagacg atggatttgt atggctaaca aaggatggag gaaacagctg gaacaacatt
     9121 acaggaacgc ttcctgccga tttcactgtt cgcagagtac aggcttcacg ttttgttgag
     9181 ggaagagtat atgtttgtct gaacggtcat acccgtgatg atttttcaac catggtgttt
     9241 tcttccgaag attatggaaa aacctggtca cgaattgcca ctgatatttc cggggaagca
     9301 gtgaacgtta tacgtgaaga tcctgccaat gaaaatttaa tttatgttgg aacagaccat
     9361 ggtgtatatg catcactcga caaaggaaaa tcttttcaac tactaaacac caattttccc
     9421 aacgttcctg ttcacgattt ggtaattcat ccccgcgaca aggagttggt tgtaggaaca
     9481 cacggccgct ctgtatacgt tgcaaatatt gcacccttgc aagaactcac aacagtagtg
     9541 caatcacagt cgctctatct gtatacaccc aaagaaatta cccattcacg gatgtgggga
     9601 aaaagctgga gcaaatggga aaaaggatat gaacctgaaa tagcaattta tttctggaca
     9661 actgcagccg gaagtgcaaa cattgaactt cgcgaggcaa gcggtaaact ggtttatgcc
     9721 gacaaaacac aacaccaaaa aggactgact cgttttatgt acaatctgaa aaacaatcag
     9781 gaaaaaaccg aaaacggaaa gaatgaattt ctcaagcctg gtgattataa actaagcgta
     9841 agcagcggag cgtctaccag taccacaaca ttaaaaatta cagagaatcg gtgaatagta
     9901 aaaggtgaac ggcaaaatga aaatttagat ctctttccca gtctcgttct ctaaacgcgc
     9961 actccgatca cacagacatc atcaatctgt tccatgtcgc ctttccaggt gtgaaaagcg
    10021 gtatgcagaa catctcgctg ctcttgcatt gatagatgtc cgttttgaaa aataagtttt
    10081 ttgaattgag actgcttata tttttttccg ttgggaccac caaactgatc tgcaaaacca
    10141 tcagtaagaa tatacaagct ttcgcccttt tgcagcttca gtttgtgttt cgtaaatggc
    10201 tctgaataat gctctaaaaa gtttccaacc ggttgtcgat cgggtttaat ttcgaatacg
    10261 gcaaaagaac tttcgggatc ttctattgca agatgttttt caggcaatga gccttttgca
    10321 gcaaaaagga acattgaatt gtttgcgccg gcatattgca attctacgct tccatccggc
    10381 aggcgtctta tggaacaaag cgcaatgtcc attccatctt ttaccgagct gacatccctg
    10441 cgctgacgca atgtttcgtt taccaataaa ttcaacttat ctaaaatagc tgccggttcc
    10501 cgtaaaccaa attcctttac acaccggttc agattattat tggcaacaat actcacaaaa
    10561 gcgcccggaa ctccgtgccc tgtacaatct actgctgcaa aaagtgataa gtcaccaaat
    10621 tgttcagccc aataaaaatc accgcttaca atatcttttg gtaaataata aataaagtat
    10681 tcaggcaaaa atccatccat aaattctttt gccggaagaa ttgcttcctg gatatgttgt
    10741 gcataccgaa tagagtccag aatctctttg ttcttttctt caaccaattc tttctgttcg
    10801 gaaatgattc tgttcgcccg cttacgcgcc tgaagtcctc gccacacaac aagcagaatt
    10861 agcatacaca acgccaatcc tattcccata taaattttct gcaggcgctg acgtttatta
    10921 ctttcttcat gaacgatgtc gcgacgttgt tgctccagca tcatttcacg ctcccgctga
    10981 tcgaaaccat aacgcatttc ttcctcaaca agcttggcag tattttcaga gttgtacaaa
    11041 ctatctttta tagtgacaaa ctcgcgatag tacttcaacg cactatcagc atttcgttgt
    11101 ccttcgaaaa cattaaacaa cgtaaaggca gaatttttta tcccttcagg agaacctact
    11161 gtagcggaaa gatcataact ccttcttgca aactcaagcg ctttcttaaa atccttttgt
    11221 ttaacataaa cctgtgcaat cgagttgtac gtatcaatct gaccctgcac atcctgcgca
    11281 cgattacgaa ttggaagtga ataaaagaaa tgatacagcg ccttgtcata atcgccttta
    11341 tcagcatatg caccgcccat gttgttacgc acagtggcga ccataaattc atcattcatt
    11401 cgttgagcac cttccaatgc ctgctcataa atctcaattg atttatcaaa ttgttcggtc
    11461 ttcaaataaa cttcagccat attggccaga gcagtattaa tacctttttg attgccaagt
    11521 tccttttgta tagctaaaac tttatcatac aacgcaagtg ctttatcata ttcctgtcgc
    11581 ttggtatgaa taagcccaat gttgttgtaa gcaatcgaca ttccttgttt gtcactgagt
    11641 cgttcaaaac aactgagtgc tttgtaataa tattctatag aacgggcgtg ctgtccgatt
    11701 ctggaaaata caattccgta accgttatag gtactcgcca acccatccag atcacctgcg
    11761 cccaaacaat tttgttcgga ttgctgataa gcgtccaatg cactattgta ttcgcctttc
    11821 atgataaagg ctactgcttt gttgcggaga atttgtccga gccctttact cgctcctttc
    11881 ggggcaaatt ccccaatgga aaaattgcag agtgaaattg cagaatcagg aacagaaaaa
    11941 acaagcggcc atgatagttc atccattgcc tttaaacgta cactatctgg ttccgcacga
    12001 ttgttataca cgctccacag agaatccgca ttgctatata cgttcccgga atgaagacaa
    12061 atcattacta tgaatagaaa gagagctcta gacatagtat caaaataggc taattttctt
    12121 tagcccatcg cggttggatt ggcctatttc gccgaatagc tcttttctgc cacaaacact
    12181 aaaaaataac tgataaagat ttacccgaga tccgctaaaa cgtggttaaa cccgttctat
    12241 ttcttccgtt caacggtata caggactaat ccctccagtg cgcggcgata ctctgattcg
    12301 ggaaacgtat gaagaatagt taatgcttta tccttgaatt catgcatacg ttttgtagca
    12361 tattgcactc ctccgctttt caaaacaaaa tcgactactt cctgcacctt tttagggtcc
    12421 tcattgtggt tttttacata gttgattatt ttgcgacgta cgcctttctc tgcattattt
    12481 aaagcgtaaa tcagtggcaa tgtcattttc ttttccttga tatcaatccc tgttggtttt
    12541 ccgatatcct ctccggaacc aaaatcgaaa atgtcgtctt tgatctgaaa ggccattcca
    12601 acgtgctcgc caa
//
LOCUS       DAOMYG010000024        12525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1688_length_12525_cov_9.108821, whole genome shotgun sequence.
ACCESSION   DAOMYG010000024 DAOMYG010000000
VERSION     DAOMYG010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 12525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12525
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1688_length_12525_cov_9.108821"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="PK610_01555"
     CDS             <1..1218
                     /locus_tag="PK610_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ34637.1"
                     /translation="FYEDKKREFNMRSFGFRASSIFTFDQNIAYKPLDSLFIPEHINP
                     TTGYQIVDATKPADSYSASNKLIAYYLGINLPVTKKMNVYTGVRVEDNVQTLNSFDRV
                     TSERVNTIIDKTDFLPSLNATYNFTEKSLVRVAFAKTINRPEFRELAPFPFLNFDFNA
                     LYYGNDTLVNCYIQNFDARYEFYPSPGETFSIGGFYKKFQNPIENIIIPTGGQNDFSY
                     QNALGAVSYGIELDTRKSLAKFGEKIKVKFISDLSIVMNASYIWSKVELNTAGFQANE
                     RPMQGQSPYIVNGGFYYQSDTLDLGISLLYNVIGPRIIFVGNDLDPDVYEMPRNILDL
                     SITKGIGQNLKIRFSINDILNQPILFIQDGNGNGKLERDADVHFMNFRRGSYYTLGVT
                     YRLNGKEPRKPKA"
     gene            1405..3999
                     /locus_tag="PK610_01560"
     CDS             1405..3999
                     /locus_tag="PK610_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ34638.1"
                     /translation="MKKLLFSICAFVGLALTANAQIIVPGGNITTNTTWTANNVYILD
                     GFVYVKPGATLTIEPGTLIKGAKTPDGSALGYRAGCLIIEPGGKIYAQGTPERPIVFT
                     STQNPGLRQPGDWGGIVLAGYAPVNVAGGIGTAEGGIGTTYGWGLATNPYGVLNAAST
                     ADSSGVLSYVRIEYPGIPLTTTANSEINGLTLYGVGSRTKVDHVQVSFSGDDAFEWFG
                     GNVNCKYLIAIGTWDDHFDTDLGYSGKVQFAVGLSYPNLADQSQSNGFESDNNSSGSA
                     ATPVTSCVFTNISMYGPFATTTQSGVSADHRRAAHIRRNSSLSCFNSVFAGFRQGLVI
                     DGTATETNALTNNTCRWENNTIAGWVLNADSLLVANGATLANVGGWFNNPTRNNQFIR
                     LNSGLMVADAFNLTDPDFTLMAGSPLSGSGSFSNAYLADPFFTPVTYRGAFGTTDWTK
                     CWANFDPQNTVYTGTIDNTVTSTITTTGNPVFCAGGLPLDLSANSVANATYSWSNGAT
                     ANTIYVTTSGNYTVTITNAFGCSATSAPFAVTVNPLPTVSISASGATTLCTGGTVDLI
                     SSQTGSGNVWSNSATTNTITVNSTGTYSVTYTDGNGCSAQSNNINVNISAAPQPTIST
                     GGSTTICSGSTATLTASTSDSYQWNLNGSPISGATSQTYAATTAGNYTVTVTNADPCD
                     GVGTSDATAVFVNPTPTASFAYSINGNTVTFTNNSLGATSYAWTFGDGNTSTSPNPSH
                     TYTTGGNYTVTLTATFGACTDVETINITSVSVEEITATVFSGVILYPNPSNQMATLEV
                     DMNMEGDVIVEVLDLRGKVVASEIRNNVYAGKQIININSSEIANGLYLVSVRSGEARQ
                     MIRMVVQH"
     gene            complement(4144..4827)
                     /locus_tag="PK610_01565"
     CDS             complement(4144..4827)
                     /locus_tag="PK610_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ34639.1"
                     /translation="MSEPSILIVDKDYGFASDTKDFLVKNGFKVNLALSFQKGLELAL
                     KHIPVLIICELDLPERDGVEFCREIRSVGLMRQSYFMLLSSRNDNFVQILAYNSGADE
                     FLTKPISERLLLSKIQAIFRRLESGSGPVNNELMGDHLRIDFERYLAIRGTREIELPK
                     KEFQILTLLYKNPKRVFSREEIKNEIWEDSADVKSRTIDVHVKKLREKIGEGLIKTIK
                     GVGYKFDQG"
     gene            4899..5783
                     /locus_tag="PK610_01570"
     CDS             4899..5783
                     /locus_tag="PK610_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HRJ34640.1"
                     /translation="MIAYNPKTWFAQIFLFHRSDTLRILWQEMLLIGLYAGGLTWALT
                     TYFPDNTHFRDTLSVHSLVGFVMGLFLVFRTNTAYDRWWEGRKQWGSLVNVSRNFSIK
                     IKSMISENDPEQEWILNLIAIFPFVAKDHLRLKGGEKINYTNEFDKTLMAEIQHWDHA
                     PSGVVYAITKRIHQLHSNGKLSGEQLIILDKECTQLLDMLGACERIRSTPIPYSYSMF
                     IKKFIFIYCITLPIGLVLDFYYWSIPVTMFIFYIIVSLEILAEEIEDPFGSDDNDLPT
                     DEIADKINANIGEIKIKQ"
     gene            complement(5978..6949)
                     /locus_tag="PK610_01575"
     CDS             complement(5978..6949)
                     /locus_tag="PK610_01575"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRJ34641.1"
                     /translation="MRIEGFMINPKINYSIQLSFSRGDMDWVDNDNSAVNSSPNVVRD
                     AVVIYRPNSNWSFTFGQTKLPGNRQRVVSSGELQFTDRSIVNATFNIDRDFGAQFSYQ
                     NSISTIGYALKGALTSGEGRNSSASDAGLAYTGRVEILPLGTFTNKGDYFEGDLEREK
                     KPKISIAGGYHYNENAMRTAGTLGRDLYEKRNISSLMMDVLFKYKGFALCGEFISRNT
                     NDPVTVNSSNQTRVVYVGDGKMMQLSYLFKNNMEVAGRYAIVTPKSSIYNSELQRQEM
                     GIGVTKYLKKHRVKLQGQLFYHQNDNLQTESVVNKYYSCVFQVELGL"
     gene            complement(7445..8845)
                     /locus_tag="PK610_01580"
     CDS             complement(7445..8845)
                     /locus_tag="PK610_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRJ34642.1"
                     /translation="MEISTSILVLLIVCVLFACLFEFINGFHDTANAVATVIYTNTLK
                     PQYAVVWSGIWNFLGVFAGGIAVAMGIVDLLPVEMLIEQSTASSVAMVLALISGAIIW
                     NFGTWYFGIPASSSHTMIGSILGVGLAFSLTPGQEFGSGVNWSKATDIGSSLLVSPLF
                     GFGMAMILMFSLSQFVNNKIIFQSPKGKEKPPMWIRAILMLTCTLVSFFHGSNDGQKG
                     VGLVMLILIGILPAYYALDANVKPGDYVGNLNSTKEILLKVNPDDLAAGDRKTYGKLM
                     DNISKLEVQMVGANTVADIPHGERFAFRSELLFITRKLEKMIKNEVLPLPVEDLKELK
                     NHSKKLRSMTDYAPTWVLVIIALSLGIGTMVGWKRIVVTIGEKIGKAHMSYAQGASAE
                     MIAASTIGLSTYFGLPVSTTHVLSSGVAGAMVAQDGIKNLQPKTVRTILMAWILTLPV
                     TMILSGSLFLLFRMAI"
     gene            9271..9579
                     /locus_tag="PK610_01585"
     CDS             9271..9579
                     /locus_tag="PK610_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34643.1"
                     /translation="MMETENGNSLKATKKKFDKAIFAAFFAGVVLIATAVALKSIKGE
                     SQFTLVLIAAMVFLLASVLMFMKSYYTGKKYAVGAKKRRKKYLTVMYGESSVFSKATK
                     "
     gene            complement(9629..10132)
                     /locus_tag="PK610_01590"
     CDS             complement(9629..10132)
                     /locus_tag="PK610_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34644.1"
                     /translation="MNKYSKIVLFLLGLTGFMACLKPEEYPIEPAIEFLRFERYGDTG
                     VVLINFTDGDGDIGLTETQDDPPYDTSSIYYHNLFIDYYEKVNGVFVQGQSIPAGDPI
                     AFNFRVQNITPQGQNKALKGEIRVTLSPSFYNPFSSNSDTIMYKIRLCDRALHMSNEV
                     ESPEIIR"
     gene            10163..11155
                     /locus_tag="PK610_01595"
     CDS             10163..11155
                     /locus_tag="PK610_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HRJ34645.1"
                     /translation="MTSKSDVLKFKLDQVNDPDSFNGVALEIFHYQRENNETYKDFLK
                     HTNRFRIEPAHWTDIPCIPVSFFKTLDVRTGSWKPETIFTSSGTSGHTTSKHPVKDTE
                     FYLQNTRIAFENKYGVLSDLTLLALLPGYLERQGSSLVAMADYFIRESKEQSGFYLNQ
                     HQELAAVLNNLKIKNEKVILLGVTHALIDFANAFPINFPELIIMETGGMKGRKKEMLR
                     SEIHLQLKRAFGVPAIHSEYGMTELFSQAYSKGDGIFHSSDTLRIRTRELSDPFQLLP
                     HGSSGAINIYDLANIDTLSFISIDDLGRTFDEYSFEVLGRIDNCDIRGCNLMVV"
     gene            11214..11741
                     /locus_tag="PK610_01600"
     CDS             11214..11741
                     /locus_tag="PK610_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34646.1"
                     /translation="MKRLLIILPFAFSLLLFACAKKTAFTSSIREKYTLSDAEIRKIQ
                     FFTSDDIILLKSENQSGRETKQGELVVSSKTSEDRVLIKKGTPGVIISQKGNTYRISF
                     DTDDRTLPFEAKSDFSAFYLTSDTLIGNKPTVRYGTNQYTISLGNSSVFLQFKLKKLK
                     KYKTNDQVVKGRKID"
     gene            11888..12277
                     /locus_tag="PK610_01605"
     CDS             11888..12277
                     /locus_tag="PK610_01605"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-binding protein"
                     /protein_id="HRJ34647.1"
                     /translation="MKTIALIIALTFSGSLMAQNGTLASGMEKPVVEKLNNGTIKATY
                     YYENGNVRETGYFLNGQKHGTWVSFDEKGNKQMEANWKNGEKDGNWTVWHENGTLHYL
                     MVYEGGKRVVSTKWDSNGALIAGNQTR"
     gene            complement(12325..>12525)
                     /locus_tag="PK610_01610"
     CDS             complement(12325..>12525)
                     /locus_tag="PK610_01610"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRJ34648.1"
                     /translation="GGAEIYRQAIDAGIVDRIYLTLVDGTFDADAYFQFDTSNWIEKS
                     SLRFDKDDKNPFSMTFFVYEKA"
BASE COUNT         3929 a         2656 c         2312 g         3628 t
ORIGIN      
        1 ttctacgaag acaagaaaag agaatttaat atgagaagtt ttggtttcag agcttcaagt
       61 atttttacat tcgatcagaa tattgcatac aaaccacttg attcactttt tatacctgaa
      121 catattaatc caacaacagg ataccagatc gtggacgcaa caaaacctgc tgactcctac
      181 tctgcttcca acaaattgat tgcctattat cttggaatta atcttcccgt tactaaaaaa
      241 atgaatgtat acaccggtgt tcgtgttgaa gacaatgttc aaacactgaa ttccttcgac
      301 agggtgacat ctgaacgtgt aaatacaatt attgataaaa ctgatttcct accctcactc
      361 aatgcaacct acaacttcac tgaaaaatct ctggtacgag ttgctttcgc caaaaccatt
      421 aacagacccg aattcagaga gcttgcacca ttcccgtttc ttaattttga ctttaatgct
      481 ctgtattacg gaaatgacac acttgtaaac tgttatatcc agaattttga tgcgcgttat
      541 gaattctatc cttctccggg tgagactttt tcgattggtg gattctataa gaagtttcag
      601 aatccaattg aaaatattat tattccaaca ggagggcaaa atgacttctc ctatcaaaat
      661 gcattaggag ctgtttcata cggtattgaa ttagacacca gaaagtcact ggcgaaattc
      721 ggagagaaaa tcaaagtgaa atttatttca gatctaagta ttgtaatgaa tgcttcctat
      781 atctggagta aagttgaatt aaacaccgca ggttttcagg ccaatgaacg cccgatgcaa
      841 ggtcaatctc cttacattgt aaatggtggt ttttattacc aaagcgatac gcttgacctg
      901 ggcataagcc ttttgtataa tgttattggt ccgcgaataa tttttgtagg gaacgacctg
      961 gatcctgatg tgtatgaaat gcctagaaac attttagacc tttctattac aaaaggaatc
     1021 ggacagaatc taaagatccg tttttcgatc aacgacatcc tgaatcaacc catccttttt
     1081 atccaggatg gaaacggaaa cggaaaactt gaacgcgatg ctgatgttca ctttatgaat
     1141 ttcaggagag gtagttatta caccttggga gtaacctatc gcttaaacgg gaaagaacca
     1201 cgtaaaccca aagcctgata caggtttatt cattgatttt taaaagggct ctggtttaga
     1261 gtcctttttg tttacgttga gataacaatg gctctgagtt attgttaaca tacaactacg
     1321 ggttcttaac ggtattataa ccaaaacaag ggaatcgctt aataggctta ggatactttt
     1381 gttttcgaat caaacttcaa aacaatgaaa aaactgttat tctcaatctg tgcctttgta
     1441 gggctcgcgc tcacagcaaa tgcacaaatt attgtaccgg gtggtaacat tactaccaac
     1501 accacctgga cagcaaacaa tgtgtacatt ctggatggct ttgtttacgt taaaccaggg
     1561 gcaactttaa ctattgaacc cggaacactt attaaaggag caaaaacacc ggatggttct
     1621 gcgcttggat accgtgcagg atgtttaatt attgaaccag gcggaaaaat ttatgctcag
     1681 ggaacacccg aacgtcctat tgtatttaca tcaacacaaa atcccggatt acgtcaaccc
     1741 ggcgactggg gaggcattgt acttgccggt tatgctccgg taaacgttgc aggcggaatt
     1801 ggaactgctg aaggtggaat tggaaccact tatggttggg ggcttgctac caatccttat
     1861 ggagttttaa atgccgcatc aactgcagac tcctctggtg ttcttagtta tgttcgtatt
     1921 gagtatcccg gaattccgct tactacaacg gctaattcag aaattaatgg tcttacatta
     1981 tacggtgttg gaagtcgtac aaaagttgat catgttcagg tttctttttc cggagatgat
     2041 gcttttgagt ggtttggtgg aaatgtaaat tgcaaatacc taattgctat tggcacttgg
     2101 gatgatcatt ttgatactga cctcggttat tcaggtaaag ttcaatttgc agttggacta
     2161 tcttatccta accttgccga ccaatctcaa tcaaatggat ttgaatcaga caacaactct
     2221 tcaggttcag ctgcaactcc ggttacatct tgtgtattca ccaatatctc aatgtacggt
     2281 ccttttgcaa caacaactca atccggcgta agcgcagacc accgtcgtgc tgcacatatc
     2341 cgcagaaact cttcattgtc gtgtttcaat tcagtatttg ccggattccg tcaaggactt
     2401 gtaattgatg gtacagctac agaaacaaac gcattaacaa acaatacttg tcgctgggaa
     2461 aacaacacca ttgcaggttg ggtattaaat gctgattcat tattggtagc aaatggagca
     2521 acgcttgcaa acgttggtgg atggttcaat aatcctacaa gaaacaacca atttattcgt
     2581 ttgaattcag gtttgatggt tgcagatgca tttaatctta ctgaccctga cttcacatta
     2641 atggcaggat caccattgtc aggtagcgga agtttttcaa acgcatacct tgcagatccc
     2701 ttctttaccc ctgttactta ccgtggtgct tttggtacaa ctgactggac aaaatgttgg
     2761 gcaaattttg acccacaaaa cacagtctac accggaacta tcgataacac tgtaacctca
     2821 acaattacaa ctacaggtaa ccctgttttc tgtgcaggcg gactaccact tgaccttagc
     2881 gcaaacagtg ttgccaatgc aacctatagc tggagcaacg gagcaactgc aaataccatt
     2941 tatgttacca cttcaggaaa ctacactgtt acaattacaa atgcgtttgg atgttctgca
     3001 acttctgcac catttgctgt tacagttaat ccacttccaa cagtttcaat ttctgcttca
     3061 ggtgcaacaa cgctttgtac cggtggaaca gttgatttaa tctccagcca aaccggctca
     3121 ggaaatgtat ggtcgaacag tgcaacaaca aatactataa ctgtgaattc tacaggaaca
     3181 tactctgtaa cctacactga tggaaacggt tgctcagctc agtcgaacaa cattaacgta
     3241 aacatttctg cagcacctca gcctactatt tcaactggcg gatcaacaac aatttgttcc
     3301 ggttcaaccg ctacacttac agcttcaaca tcagatagct accagtggaa tctgaatggt
     3361 tcaccaattt ccggtgctac ttcccaaact tatgctgcaa caactgcagg taattacaca
     3421 gtaacagtta ccaatgcaga cccatgtgat ggtgttggta catctgatgc tactgctgtt
     3481 ttcgtcaacc ctacacctac agcatcattt gcatacagca tcaatggcaa tacagttaca
     3541 ttcaccaaca actctcttgg cgcaacatct tacgcgtgga catttggtga tggtaatact
     3601 tcaacttcac caaatccaag tcacacttat actaccggtg gaaactacac tgtaacacta
     3661 actgctactt tcggtgcttg tacagacgtt gaaacgatca acatcacaag cgtttctgta
     3721 gaagaaatta ctgctactgt attctccggt gtaattcttt acccaaatcc ttctaaccaa
     3781 atggcaacgc ttgaagttga catgaacatg gaaggtgatg taattgtaga agtgcttgac
     3841 cttagaggta aagttgttgc ttctgaaatc cgcaacaatg tttatgcagg caaacaaatc
     3901 atcaacatca acagttcaga aattgccaac ggtttatatc tggtaagcgt tcgttcaggc
     3961 gaagcgcgtc agatgatccg catggtagtt cagcactaat catacacccc aaagaaaaag
     4021 ggtcgtctgc aaggacgacc cttttttatt tcaccttaac ctgaagatgg tgttcagtta
     4081 atcgcaaaaa aaaccatgtt cgttcaaaac atagtgagtg gatttatcaa gaaaattatt
     4141 tatttaccct tgatcaaact tataaccaac tcctttgatg gttttaatca agccctcacc
     4201 tattttctcg cgaagttttt ttacatgcac atcaatcgtg cgacttttca catctgcact
     4261 gtcttcccag atttcgtttt taatttcctc tcttgaaaaa actcgtttgg gatttttgta
     4321 taacaaggtt agaatctgaa actccttttt gggcagttca atttctcttg taccccgtat
     4381 tgccaggtaa cgttcaaaat caatccttaa atgatcaccc atgagctcat tgttgaccgg
     4441 tccggatccg gattccagtc gacgaaaaat ggcttgtatt ttactaagta ataaacgttc
     4501 gctaattggt tttgtaagaa attcgtcggc cccggaatta tatgccagga tctgaacaaa
     4561 attatcattc cttgaactga gcagcataaa atacgactga cgcattagtc caactgatct
     4621 tatctctctg caaaactcaa cgccatctcg ctctggaagg tcgagttcac atattattaa
     4681 tacaggaatg tgtttcaatg ccagttccaa acccttctga aaggaaagag caaggtttac
     4741 cttaaatccg tttttcacaa ggaaatcctt ggtatcagag gcaaatccgt agtctttgtc
     4801 gacgattaat atgctgggtt cggacatgta attatttgcc attaaattag catcgcaata
     4861 caagctcctc tttaactaca tcttaagttt gagttaacat gatcgcctac aaccccaaaa
     4921 cctggtttgc acaaatattt ctgttccacc ggtcagacac ccttcgtatt ctttggcagg
     4981 aaatgctgtt aattggactt tatgcaggag gactaacctg ggctctaacc acctattttc
     5041 cggataacac ccatttcagg gacacccttt ccgtacattc ccttgtgggt tttgtaatgg
     5101 gtttgtttct tgtttttaga acgaatacag catacgaccg ttggtgggaa ggaagaaaac
     5161 aatggggatc ccttgtaaac gttagcagaa acttttcgat aaagatcaaa agcatgatca
     5221 gcgaaaacga tcccgaacaa gagtggatac ttaatctgat tgccattttc ccctttgtag
     5281 cgaaagatca tttgcgatta aagggtggag agaaaataaa ttatacgaat gaatttgaca
     5341 aaacattgat ggctgaaatt caacattggg atcatgcgcc ttccggagtt gtttatgcaa
     5401 taaccaaaag aattcaccaa ttacacagca atggaaaact aagcggtgag caattaataa
     5461 ttcttgataa agaatgcacc caacttctcg acatgctagg cgcctgtgaa cgaataaggt
     5521 ctacgcctat tccttattcc tacagcatgt tcatcaaaaa atttattttc atttattgca
     5581 tcaccttacc aattggactt gtactggatt tctattattg gtccattccg gtaaccatgt
     5641 tcatttttta tataatcgta agcctggaaa ttcttgctga agaaattgaa gacccttttg
     5701 gaagtgatga taatgattta ccgacagatg agattgcgga taagataaat gccaacattg
     5761 gtgaaataaa aatcaaacaa tgattataag ctattcaatg aattataaca atacaacgga
     5821 tttaaacaaa agccccctta agtagccaga atcacttatc cttgttccgc ttctgtcaaa
     5881 tggttaaaaa gaaagaactt ctgtttcatc aagaatagag cgttttcaaa taaagaattg
     5941 gaagtaaaaa gaaaccacaa tcacgccggc aatgttctca taatccaagc tctacctgaa
     6001 atacgcaact gtagtattta ttgacaacac tttctgtttg aagattatca ttttgatggt
     6061 aaaataattg cccctgcaat ttcaccctgt gcttcttcaa atatttcgtt actccaatcc
     6121 ccatttcctg acgttgaagt tcgctgttgt aaattgaact tttaggagta acaatagcat
     6181 atctcccggc aacttccata ttattcttaa acaggtagct taattgcatc atttttccat
     6241 ccccaacata aacgaccctt gtctgatttg aactattaac agttacaggg tcattggtat
     6301 tgcgtgaaat aaattctcca cataatgcaa agcctttata cttaaaaagg acatccatca
     6361 ttaatgagct aatgtttcgc ttttcataca agtctcttcc aagagttcca gcggttctca
     6421 ttgcattttc attataatga tacccaccgg caatagatat ttttggtttt ttctctcttt
     6481 caagatctcc ttcaaaataa tctcctttgt tggtgaaggt acccaaaggc aaaatttcta
     6541 ctctccctgt atatgccagt cctgcatctg atgcagaaga attccttcct tcacccgaag
     6601 ttaaagctcc ctttaaagca tacccaattg tagaaattga attttgataa gaaaactgcg
     6661 ctccaaaatc acggtcaata ttgaatgtgg cattcacaat cgaccggtct gtgaattgca
     6721 attctcctga cgaaacaaca cgttgcctgt taccaggaag ctttgtttgt ccaaacgtaa
     6781 acgaccaatt tgaatttggc cgatatatca ccaccgcatc acgaaccaca ttcggactgg
     6841 aattaactgc ggagttatca ttatccaccc aatccatgtc tcctctggag aatgaaagct
     6901 gaattgagta attgattttt ggatttatca taaaaccttc aatcctcatt cgaagccttc
     6961 ttacacgagc ttcaatttcg gaagcactaa gatcacttgt tgagttggag acatagcccg
     7021 cacgcaactg ggacctgaat cgaaaattta atgagaaaga gctatccgga actgcaatac
     7081 ctattcctcg ggaaaaatta aatgttggcg cattatttgc gctttgtgct atcattgatt
     7141 tattaaacag aaagaggatg ataattgcga gtaagcgcag ggataaactt ttcatgattc
     7201 gttgatttga aataggaagg gttaatgatt ctgtatgaaa acaataccct caaaaccgga
     7261 acccgacaca ggttgcattt tcataataaa tgaatggatt gttctcaaaa cattaccaat
     7321 attaattctg tgcgaatcta ttggagcaat atcgccacat tgttaatcca atgttaaatt
     7381 aatgtgacga aaaaaggacc gctacaatgt gcggtccttc aaatggattg aatatccggt
     7441 tctatcaaat agccattctg aatagcagga ataatgatcc ggaaaggatc atggtaaccg
     7501 gcaatgtcag tatccaggcc atcaagatgg tgcgtactgt ttttggctga aggtttttaa
     7561 taccgtcctg agccaccatt gctcctgcaa cacccgatga tagcacatgt gttgttgaaa
     7621 caggtaatcc gaaataagtg ctcaatccaa tagttgaagc agcgatcatt tcagcactag
     7681 ctccctgggc atagctcatg tgggctttac caattttctc accaatggtc acaactatac
     7741 gtttccaccc aaccatggtg ccaataccaa gcgataatgc aataattacc aaaacccatg
     7801 taggtgcata atctgtcatg gacctaagct tcttggaatg attcttcaat tctttaagat
     7861 cttcaacggg caaaggaaga acttcattct tgatcatttt ctccaattta cgcgtgatga
     7921 acaacaactc acttctgaaa gcaaaacgct caccatgggg aatatctgca actgtatttg
     7981 ctcctaccat ctgcacttca agtttggaaa tgttatccat caactttccg tacgttttgc
     8041 gatcgccggc ggctagatca tccggattga cttttaacag aatctctttt gtagaattta
     8101 aattcccgac ataatcaccg ggttttacgt tggcatccaa agcgtaatag gcaggtagaa
     8161 taccaatcag aattaacatc accaatccaa cacccttttg tccgtcgttg gaaccatgaa
     8221 aaaaacttac aagcgtacag gtcaacataa gtattgcgcg aatccacata ggtggtttct
     8281 cttttccttt tggcgactga aaaataatct tgttgttgac gaattgcgaa agtgaaaaca
     8341 tcaaaatcat cgccattcca aaaccaaaaa gaggagaaac caataatgac gatccaatat
     8401 ctgttgcttt actccaattc actcccgaac caaactcttg tccaggtgta agcgaaaacg
     8461 ctaatccaac tcccaaaata gaaccgatca tggtatgcga actggatgca ggaattccaa
     8521 aataccatgt tccgaaattc caaatgatcg ctcctgaaat cagcgccagt accattgcaa
     8581 cactacttgc cgtactctgc tcaattaaca tttcaaccgg caggagatcg acaattccca
     8641 ttgctactgc aattcctcct gcaaacactc caaggaaatt ccagataccg gaccaaacta
     8701 ctgcatattg cggttttaag gtgttggtat aaattacagt ggcaactgca tttgcggtat
     8761 catgaaatcc gttgatgaat tcgaacaaac atgcgaacaa cacacaaaca attagcaaca
     8821 cgagaatgga cgtactgatt tccatttttt tcttttttta ttcgggagca aatatctgat
     8881 aatcctttca tcgcaaatcg accggataat gaaccccgat agccacttaa cattacctta
     8941 atattggcgt gatgtttgca aaacattggc gcgcagtgga aaattgacaa cttcttttca
     9001 aaacactgtt atttaacaga ttaacggcag tttaatagat ctatctaact tttcaactct
     9061 ctgagcaact ataaaaaagt cgtctgcatt taataaaaaa ttaaccgcaa ggctgcaaaa
     9121 aaccgatgcc agggcagaaa acaagcttca caccccatag ttatatcatt aaaaatcaac
     9181 attttgtaca gccaatgggg cagattgaaa ctattaggct agaaatacgt tacatatgtt
     9241 aactttgtgt tatctaatac tgtaacgaaa atgatggaaa ctgaaaacgg aaactcactt
     9301 aaggctacga agaaaaaatt cgataaagcc atatttgcgg catttttcgc aggtgttgtt
     9361 ttgattgcaa cggcagttgc acttaagagc ataaagggag agtctcagtt cacacttgtt
     9421 cttattgctg caatggtgtt cctactagcc tcggttctga tgtttatgaa atcctactac
     9481 accggtaaaa aatatgctgt cggcgctaaa aaacgtcgga aaaaatacct tacagttatg
     9541 tacggagaaa gcagcgtttt cagcaaagcg acgaaataat ctcccttata atattgggaa
     9601 aagcgacgga aacacctgtc gctttttttt atcggataat ctcaggagac tccacctcat
     9661 tactcatatg caaagcccgg tcgcacaacc tgatcttata cataatggta tcggaattgg
     9721 atgaaaaagg attgtagaat gaaggcgata acgtcactct gatttctccc ttcaatgctt
     9781 tgttctgccc ttggggcgta atattctgta cacggaaatt aaatgcgatc ggatctcctg
     9841 ccggaattga ttgtccctga acaaaaacac catttacctt ttcgtaataa tcaataaaaa
     9901 gattatgata ataaatactg gaagtatcat agggaggatc atcctgcgtt tcagttaatc
     9961 caatatcacc atctccgtct gtgaaattga taagcacaac tccggtatca ccgtagcgtt
    10021 caaaacgtaa aaattcaatt gcaggttcaa tggggtattc ttcaggcttc aaacaagcca
    10081 taaatcccgt aagccccaaa aggaatagca ctatctttga atacttgttc atccgactat
    10141 aaagttacaa aatcctgtac gattgaccag caaaagcgat gtactaaaat ttaaacttga
    10201 tcaggttaac gatcctgata gttttaacgg tgttgcgctg gaaatatttc attaccaacg
    10261 ggaaaataac gaaacttata aggatttcct gaaacatacc aatcgctttc gcattgagcc
    10321 cgctcattgg accgatattc cttgcatccc cgtttccttt ttcaaaacac tcgatgtacg
    10381 aacaggtagt tggaaacctg agaccatatt cacttccagc ggaaccagcg gtcatactac
    10441 aagtaaacat cctgttaaag acactgaatt ctatttgcaa aatacacgta ttgcctttga
    10501 aaataagtac ggtgttctga gtgatctaac gctactggcc ttattacccg gttacctcga
    10561 gcgacaagga agttcattgg ttgcaatggc cgactatttc atccgggaat caaaagaaca
    10621 aagtggattc tacctgaatc aacatcagga gctggctgcg gttctgaata atttaaagat
    10681 caagaatgaa aaagtaattc tacttggagt aacacatgca ttgattgatt ttgcgaatgc
    10741 atttcccata aattttccgg aactgattat tatggaaaca ggcgggatga aaggaagaaa
    10801 aaaagaaatg cttcgttctg aaattcattt acagctaaaa agagcatttg gcgttccggc
    10861 aattcattcg gaatacggaa tgactgaact attttcccag gcatacagta aaggagacgg
    10921 catattccat tcatccgata ccctgcgtat ccgcacacgg gaactttctg acccatttca
    10981 gttattaccc catggaagtt caggtgccat aaatatttac gacctggcta atatcgacac
    11041 cctttctttt atcagtatag atgatttagg gcgtacattt gacgaatatt catttgaagt
    11101 ccttggaaga attgacaatt gcgatatccg cggatgcaat cttatggttg tatgaacctc
    11161 atcatttacc catttggtac agttcttgaa cttttaaaat aaacaacaca catatgaaac
    11221 gccttctgat catccttcca tttgcatttt cattgctact tttcgcatgt gcaaaaaaaa
    11281 ctgcctttac ttcttctatt agggagaaat acacgttgag cgatgccgaa atcaggaaga
    11341 ttcagttttt tacttccgat gatatcattc ttcttaaaag tgaaaatcaa agcggcaggg
    11401 aaacaaagca aggcgaactg gtggtgtctt caaaaacatc agaagatcgt gtactgatta
    11461 aaaaaggaac accaggtgta attatttctc aaaaaggaaa cacctaccga atcagctttg
    11521 ataccgatga ccgcaccttg ccttttgaag caaaatctga cttcagcgcg ttctatctta
    11581 catcggatac actgatcgga aacaaaccaa ccgtacggta tggtacaaat cagtacacca
    11641 tcagtcttgg gaacagctca gtgtttcttc agtttaaact aaaaaaacta aaaaaataca
    11701 agaccaacga ccaggttgta aaaggccgaa aaatagactg attatcagtc aataactaca
    11761 aaatcgtcat cgccaacatt atcgcaatgt tatcaaaacg ctaaccaata ttaagccagt
    11821 gttaatccaa ttgaaactcc gggtaaagca ggacgtatct ttgacctata acaaacaaag
    11881 aacgcctatg aaaacgattg ctcttattat tgcgcttact ttctccggaa gtctgatggc
    11941 acaaaacgga actcttgctt ccggaatgga gaaacctgtt gttgagaaat tgaacaacgg
    12001 aacgatcaag gcgacgtatt attacgagaa tggcaatgtt cgtgaaaccg gatactttct
    12061 aaatggccag aaacacggca catgggtttc ttttgatgaa aaaggaaaca aacaaatgga
    12121 agccaattgg aaaaacggtg aaaaagatgg taactggact gtttggcacg agaacggaac
    12181 tttacactac ctgatggttt atgaaggtgg taagcgcgtt gtgtctacca aatgggatag
    12241 caacggtgca ttgatcgccg gaaaccagac acgttaaaga attatatttc aaatagaaaa
    12301 gtcctgaatt acttcaggac ttttttatgc cttttcgtaa acgaaaaatg tcattgagaa
    12361 tgggttctta tcatccttat caaaacgaag cgaacttttt tcgatccaat tggatgtatc
    12421 gaattggaaa taggcatcag catcgaacgt tccatcaaca agcgttaaat aaattctgtc
    12481 gactataccg gcatcaatag cctgtctgta aatttccgcc ccacc
//
LOCUS       DAOMYG010000025        12187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1737_length_12187_cov_10.334240, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000025 DAOMYG010000000
VERSION     DAOMYG010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 12187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12187
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1737_length_12187_cov_10.334240"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PK610_01615"
     CDS             <1..206
                     /locus_tag="PK610_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRJ34649.1"
                     /translation="TPPAMTSRSWPSGWPPRPGYHMSKKHWNTLVMDGSLPEKMILKW
                     VDDSYDLVVASLPKKLREELSNL"
     gene            250..519
                     /locus_tag="PK610_01620"
     CDS             250..519
                     /locus_tag="PK610_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34650.1"
                     /translation="MTFLRAFVCLLLFSFLSCSTGESEMEDKTDSVSTTTTESFETMP
                     ITNEYVYEITDRKTGKKKILSQEEYLNSDYTADPNVEVVEKVKIH"
     gene            complement(520..969)
                     /locus_tag="PK610_01625"
     CDS             complement(520..969)
                     /locus_tag="PK610_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34651.1"
                     /translation="MQGTISNPEEDESPLLKVRYSRTWGMTLFLVGLVLLVLYGILFF
                     RTGLLKPFHLAGVVMLVVSGLLYMRNPYFDVYPDHVLVHALIGPVKKKYPFKQLSDFI
                     VEGEKIYILFNGKKKRVWIGKMMADKEKWSQFLLLIRASDPSGELHE"
     gene            1072..1950
                     /locus_tag="PK610_01630"
     CDS             1072..1950
                     /locus_tag="PK610_01630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ34652.1"
                     /translation="MLLLSPQHWIDYELLDCGNFEKLERFGEYVTIRPEPQAVWDRKW
                     NDAEWEKRAHVLFEQQSSSSGEWKKIKKMPDQWGIDYKIQTLKNKPAVRLRLGLTAFK
                     HVGVFPEQAVNWEYIAQSISQMKTSQPRFLNLFAYTGAASLAAKTAGADVYHVDSIKQ
                     VVNWSRENMERSGLSDVRWVVEDALKFVKRELKRGNKYNGIILDPPAFGHGPNGEKWK
                     LEEQINEMMKIVCDLLDEEHMLILNAYSLGFSSIIIENLLREKAGKNLETGELFLQAA
                     SGVKLPLGVFGRFRSF"
     gene            1950..3005
                     /locus_tag="PK610_01635"
     CDS             1950..3005
                     /locus_tag="PK610_01635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ34653.1"
                     /translation="MVDVHIIGQGLAGSVAALELLRAGQTIHVSNDPSLSHCSRTAAG
                     LFNPLNFKTLSRTWNADVVFPVAMQFYKDSELQLKASFWHPLPMLRIIGSEGEQKEWK
                     AALSGNHFAPFLNNDPVPEEMSAYNRGFGLGLVEHCGYLDTNTFLEAVTAMLNDKNIY
                     SEYTCKQEPEKTNQGWNINGIESRRVLYCEGWKAMENPLFNWLPFFPVKGDVILIEDE
                     NLKLDHLINAGCFVLPLGNKFYRIGSTYYPDETDDVPTQKGREELLQKAEKVLKRPIN
                     ISNQWAGVRPSTGDRRPFIGEHPGKKGIWIFNGLGSRGVILSPWVARQLSSSMIFGTP
                     IPRETDIVRHLKRFRKV"
     gene            3134..9340
                     /locus_tag="PK610_01640"
     CDS             3134..9340
                     /locus_tag="PK610_01640"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRJ34654.1"
                     /translation="MLRFKIISSAIVVALVGNLLGNFNLKTGGSSKQIPLTAPVKNGD
                     YNELWSKVDSLEQKGLPKSSLEVVQEILSRARKEANGPQEIKATIHIMKYNSYLEEDD
                     YVGAIGNLQRMANDAKSPQKQVIHSLIAEVYWGYYQSNRYKFLGRTTVMGVKDNDIRT
                     WDLRTISERIVLHYLLSLTEQDILKSTPIESFEDILIEEKEYRSLRPTLFDLLAHRAI
                     DFFQGNEFDVTKPAYTFRLNDPNHFATADKFAAVKFSTKDSLSTKFFALHLLQQLTVF
                     HLKDENPAALIPVEIKRLQFVKNNSVVANKDTLYAAALFNLKNKYIDHSGCAEVWYEI
                     ARLHVELGNQYKALIPETEKFKWEKKRAVDICAETIKKYPGTYGADWCLSLKTQLENR
                     TLDLSAEDIVVADKPTRLVIGSTNMKKLHLRIMDVGWDFEGRGYNEEQEAYIKRILAQ
                     KTFRQFEVDLKDDGDMQRHLLEIELPSLPKGNYIVIVGSHSDYSITNEIVSWLSVGSS
                     NISYFERRSDDESQLEFTMTDRITGLPLKGVRAQLVHVEYDYSSRRYIHRKGATYTSD
                     ENGYFKVKHSSRNERNFYLDFKTNNDRLSTTRNYYQYEPWIDTKQKTHTYFFSDRMIY
                     RPGQTIHFKGIRLHTDANGENAKIGPNEKASLIFYDNNYQKIASLDLRSNEYGTFSGS
                     FTAPSGVLTGQMHITDGYGTHYFSVEEYKRPKFEADFEPVEGAYKLGQKIKVTGTAKA
                     YAGSNIDGAKVSYRVSRTTYFPYRWFYWYYDRDLSYNQMEIAFGETTTKEDGTFEIEF
                     EAIADESVSKTYKPAFNFTVSADITDINGETRSASTVVNVGYTAMTLSTSIYGEMDVS
                     SKTPVYLYANNLNGSPIDANGSVSIHKLKQPDRVLQQRRLQRPDRHQLSADEYKKTFP
                     NELYADELNIYNWAREKKVHEMRFNTKTLDSIILKDLNSWEPGHYLIETIAKDPYDEE
                     VKDITYFTVTDNKSTKMFFRNELKINADPYKQYEPGEKAIILLSTKWKDVTVLMEIEE
                     KNKIVSRQWIRLSDEQKTISIPILDKHRGNFAVHFSTVRNNRFHNQSVVINVPYTNKE
                     LDIEFETFRNKILPGSEEEWKLKIKGKKGEKLAAEMLLTMYDASLDAFASNGFYFHPF
                     NYYYAQHYWSAGDGFASRSGQQYGKDWTRYHSARGRNFDRINWFGYETYSYYGYGYNK
                     RYYMRGGYDRERVSAVTETAATGALMEEQKNGESISFPAAAKQTYAWSGDNVDDLSVA
                     EKDKDGKKSSRDESGNTRNPEGKVQVRTNFSETAFFFPQLQTDAEGKIVFKFTAPESL
                     TKWKLLGLAHTKELQTGTIEKEVITQKELMVMPNAPRFFREGDRITFTTKISNLSEKD
                     LDGTITLELIDPLTQKTVNALFAHKGEQIKFTVKKGASTSASWTFSIPENLGAVSYRL
                     VARSGSFSDGEEMAVPILTNRMMVTESMPLPIRKAGTKQFNFTKLSNSNKSSTLRHHK
                     LTLEFTNNPAWYAIQAMPYMMEYPYECAEQVFTRFYANSIASHITNSNPKIKAVFEDW
                     KRSSPEAFLSNLEKNQELKSLMLEETPWVLEAKDESERKKRVAVLFDLNRMGNELSRA
                     LGKLRNMQVSNGGWPWFPGMPESRYITQHIITGMGHLDHLGVKNIREEHSTWQMVQNG
                     VRYLDSRVIEDYNYIKRHYPNYKKEKHIGYDAIQYLYARSYFKDLSMDDNLKEALLYY
                     QDQAKKYWLDFNLYAQGMLGLAAHRFTMKELAGDIVKSIREKAIRHDELGMYWKDNVV
                     GYFWYQAPIETHALMIEMFDEVANDQETVEELKVWLLKNKQTSDWKTTKATAEACYAL
                     LLKGTDLLSNDEMVEIRMGNEIVDPAKMGAKVEAGTGYFKTSWSAEKITPEMGKITLS
                     KKKDGVAWGAVYWQYFEQLDKITPHETPLKLNKKLFLVKKTETGQQMVPVDEKTSLEP
                     GSRVRVKIELRVDRAMEYVHMKDMRASCFEPVNVFSQYKWQDGLGYYQSTRDAATNFF
                     FEYLPKGTHVFEYDLLVSHLGDFSNGITTIQCMYAPEFTSHSEGIRVQVKGQ"
     gene            9387..9971
                     /locus_tag="PK610_01645"
     CDS             9387..9971
                     /locus_tag="PK610_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34655.1"
                     /translation="MKKYLFLLALPVSLFLSSCGGETKTDENNDDSTVVSDIDYTGMS
                     EFDLEPHGLMASMMVTDIISENGSPFPVTVVRDTNMLTWDIKVGKEGNEEFYLIIEET
                     DGEGNYIKREKDRLASDDVFKEEYLTDENDLLLYKASLPEGTGQRDYYHVFGLVKIGK
                     LEYIVHSYAMGDYSEVQARDMLKGIQSLREPSAI"
     gene            complement(10214..11995)
                     /locus_tag="PK610_01650"
     CDS             complement(10214..11995)
                     /locus_tag="PK610_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ34656.1"
                     /translation="MKRLLAYTIAGSFLLSSCGGGSESGSNETEKPTRIAKGDVALGG
                     SFNLAENEKYNTLFPYEIKDAGSAHIAWQIFEGLVRMSQKDLSIQPSIAESWEVSDDQ
                     KEYVFHLRKDVRFHDDPCFADGKGRLVTATDIKFSLELACTKGISDQSYELNFKERLE
                     GAQAFYNGEAKEVSGVEVVDDYTVKIRTIAPSSAFLYILANPVASVIPKEGYEKYGKD
                     LKIGTGAFKFSTGGDAKSELILVRNENYYRVDKHGNQLPYLDTVHIYFVNEDTEELDM
                     FREGELSMIYGLPAEKISEVVQENIPDFTNKPPKYILLREPEMSTQFYELNLTRPQFK
                     DVRVRRALSMAIDRSKIMENVLNGQAAANGARQNQTGNYGLVPPLYQFSKYDTSITRG
                     YTYNPEMAKKLMAQAGYPGGKGFPTLKLLVNYGGSRNTKVASEVMRQWRDVLGINIEL
                     EQVSLAQKIEDSQYGRADIYRSAWVADYPSPESFLSIAYGKNVPATLDLPSHPNTMRY
                     KSAEFDKYYELGMAAKTEEERYANFAKAESIMMQDCPVIIIWYVENYRLLHSSVHNFH
                     NNPMSYFDLSDIYLKAPVAEKPAEKKN"
BASE COUNT         3737 a         2726 c         2583 g         3141 t
ORIGIN      
        1 gtacgccacc tgccatgacg agccgcagct ggccgtctgg ctggccgccg aggcccggat
       61 accacatgag caagaaacat tggaacacgt tggtgatgga cggtagctta cctgaaaaaa
      121 tgattcttaa gtgggttgat gattcttacg atttggtcgt cgcatcgttg ccgaaaaaac
      181 ttagagaaga gctttcaaat ctttaaggct gttcattttc atcttatctt tgcaaataaa
      241 tactacaaaa tgacttttct gcgcgctttt gtttgtttgc tgctcttctc ctttctttcc
      301 tgctcaacag gtgaatctga aatggaggat aaaacagact cagtctcgac tacaacaacc
      361 gaaagctttg aaaccatgcc catcaccaat gagtatgtct acgagattac cgatagaaaa
      421 acaggaaaga aaaaaattct gagtcaggaa gaatacctta actctgatta caccgccgac
      481 ccaaatgtgg aagtggtgga aaaagtgaaa attcactgat cactcatgca attcaccgga
      541 aggatcagaa gcacggatga gcagtagaaa ctgcgaccat ttttccttgt ccgccatcat
      601 ctttccaatc cacacccttt tcttttttcc attgaaaagg atgtaaattt tctcaccctc
      661 cacaataaaa tcggatagct gcttaaacgg atactttttc ttaacaggac caattaaagc
      721 atgcacaagc acatggtccg gataaacatc aaaataagga ttccgcatat aaagtaatcc
      781 gcttacaaca agcattacaa ctccggcaag atggaacggc ttaagtaagc ctgtcctgaa
      841 aaaaagaatg ccatagagca caagcaacac aagcccaacg aggaacaagg tcatccccca
      901 tgttcgcgag tacctaactt tcaaaagcgg cgattcatct tcctccggat tactgattgt
      961 cccttgcatt catcgaaatt agtgatttcg ttctctctgc gtatctttgt gggcgaaaca
     1021 agcctttgtt gtttgcaacg attcggcaaa ggcatccatt attcaggcaa aatgctcctt
     1081 ctttctcccc aacactggat cgattacgaa ttactcgatt gcggcaattt tgaaaaactc
     1141 gaacgcttcg gtgaatacgt aaccatccgc cccgaacctc aggctgtgtg ggatagaaaa
     1201 tggaatgatg cagaatggga aaaaagagcg catgtactgt ttgaacaaca atcgagttct
     1261 tccggtgagt ggaaaaaaat aaagaaaatg cccgaccaat ggggtattga ctataaaatt
     1321 caaaccttaa aaaacaagcc tgctgttcgt ctccgcttag gtttgaccgc attcaaacat
     1381 gttggcgttt ttccggagca ggcagtaaac tgggaataca ttgcacagtc catttcccaa
     1441 atgaaaacca gtcaaccacg ttttctaaat ctttttgcct atacaggagc ggcatctctt
     1501 gctgctaaaa cggctggagc tgatgtgtac catgtcgact ccattaaaca agtggtaaac
     1561 tggtcgcgcg aaaacatgga gcgttccggt ttaagtgatg ttcgatgggt tgttgaagat
     1621 gccctgaaat ttgtaaaacg cgaattgaag cgtggaaata aatacaacgg aattattctc
     1681 gatccgcccg ccttcggtca tggtcccaac ggcgaaaaat ggaaactgga ggagcagatc
     1741 aatgaaatga tgaaaattgt atgcgatcta ctcgatgaag aacacatgct gattttaaac
     1801 gcgtattcac ttggcttttc gagcatcatc atagaaaatt tactgcgtga aaaagcagga
     1861 aaaaatcttg aaacaggaga gctttttctt caggctgctt ccggagtaaa attgcctctt
     1921 ggtgtttttg gccgtttcag aagtttctga tggtagatgt acacatcatc ggacaaggat
     1981 tagcgggctc tgttgctgct ctcgaactat tgcgtgccgg acaaacaatt catgtcagca
     2041 acgatccttc cctttcacat tgctcccgaa cagctgcagg attgttcaat ccgcttaact
     2101 ttaaaacact gagccggaca tggaatgccg atgtagtgtt tccggtagcc atgcagtttt
     2161 acaaagacag tgaacttcaa ctaaaagctt cgttttggca tccgcttcct atgctgagga
     2221 taattggttc ggaaggtgaa caaaaagaat ggaaagcagc tttgtcaggc aaccactttg
     2281 cgccattcct caataacgat ccggttccgg aagaaatgtc cgcttacaac agaggctttg
     2341 gactgggatt ggttgaacat tgcggatatc tcgacaccaa cacattcctc gaggcggtaa
     2401 cagcaatgct caacgataaa aatatttact ccgaatacac ctgcaaacaa gagcctgaaa
     2461 agacaaatca aggttggaac atcaatggca tagaaagccg gcgtgttctt tattgcgaag
     2521 gatggaaagc catggaaaat cctctcttca actggttgcc tttttttccg gttaagggcg
     2581 atgttatcct tattgaagat gaaaacctga agctcgacca cctgattaat gcgggctgtt
     2641 ttgtattgcc ccttggaaac aaattttatc gtattggatc tacttattac ccggatgaaa
     2701 ccgatgatgt tcccacccaa aaagggaggg aggaattact gcaaaaagcc gaaaaagtat
     2761 tgaagcgccc catcaacata agcaatcaat gggcgggggt acgaccttcc actggcgacc
     2821 ggcgaccttt cattggagaa catccaggta aaaaaggaat ctggatcttc aatggactcg
     2881 gctcaagagg agtcatcctg tctccgtggg tggccaggca actttcttcg tccatgattt
     2941 tcggaacccc tattccccgg gaaacagaca ttgtgcgcca tctgaaacgg ttcagaaaag
     3001 tataatttgc gtttttcttt ttcatctgga attattccgg ggttggttgg gattttattg
     3061 aacctatttg cgctttcagt gtaccgccca ttagattttc ataccttgaa cttttcaaaa
     3121 ccttatgtcg accatgcttc gttttaaaat catcagctct gcaattgttg ttgcactcgt
     3181 tggaaactta ttaggaaatt ttaacctgaa aaccggcggt tcatcaaaac agatccccct
     3241 cactgctcca gtaaaaaatg gcgactataa tgaattgtgg tccaaagttg attcactcga
     3301 acaaaaaggg cttccaaaat cttcgctcga agttgttcag gaaatcctga gccgcgcacg
     3361 aaaagaggca aatggtccgc aagagatcaa agccactatc cacatcatga agtacaacag
     3421 ttatctcgag gaagatgatt atgtaggtgc tatcggaaat ttacaacgca tggccaatga
     3481 tgccaagtcg ccgcagaagc aggttattca ctcgctcatt gccgaagtgt attggggcta
     3541 ctatcaatcc aaccgctata agtttcttgg tcgcaccaca gtaatgggtg tcaaggataa
     3601 tgatattcgc acctgggacc tgcgtaccat ttcggagagg attgtactgc attatctcct
     3661 ttcccttacc gagcaggaca ttctcaagtc aactccgatc gaaagctttg aagacattct
     3721 catcgaagaa aaagaatacc gttcacttcg tccaacctta tttgatctgc tcgctcaccg
     3781 tgcaattgat ttcttccagg gcaatgaatt tgatgtcact aaaccggcct ataccttccg
     3841 cttaaatgat cccaatcact tcgccactgc cgataaattc gctgcagtaa aatttagcac
     3901 gaaagactct ttatccacca aatttttcgc gcttcatcta ttacagcagt taacggtttt
     3961 ccacctgaaa gatgaaaatc ccgcggcatt gattcctgtg gaaattaagc gattgcagtt
     4021 tgtaaaaaac aatagtgttg ttgccaataa ggacacactc tatgcggctg cgcttttcaa
     4081 cctgaagaac aaatacatcg atcattcggg ttgtgcagaa gtgtggtacg aaattgcacg
     4141 attacacgta gagctcggta accaatacaa agcgctgatt cccgaaactg aaaaattcaa
     4201 atgggaaaaa aaacgtgccg tcgatatttg cgctgaaacc atcaaaaaat atcccggtac
     4261 ctatggtgca gattggtgtt tatcgctgaa aacacaattg gaaaaccgca ctctcgatct
     4321 ttccgcagaa gacattgttg ttgccgataa accaacccga ttagtaatcg gttcaaccaa
     4381 tatgaaaaaa ctacacctgc gcattatgga tgttggttgg gattttgaag gacgcggata
     4441 caatgaagaa caggaagcct acattaaacg catcctcgct caaaaaacat ttcgtcaatt
     4501 tgaagttgac ctcaaagacg atggtgatat gcaacgccac ttgctggaaa tagaactccc
     4561 atcacttcca aaagggaatt atatcgttat cgttggttct cattctgatt attccataac
     4621 taacgagatt gtttcttggc tttcagtcgg ttccagcaac atcagttatt ttgaaagaag
     4681 aagcgatgat gagagtcaac tggagttcac catgactgac cgaatcacag gtctgcccct
     4741 gaaaggtgtc cgcgcacaac tcgtgcatgt ggagtacgat tacagctcgc gacggtatat
     4801 tcaccgcaag ggagcaacct atacttctga tgaaaacggt tacttcaagg tgaaacacag
     4861 ctcacgcaat gagcgcaatt tttatctcga tttcaaaaca aacaacgacc gcttaagcac
     4921 aacacgcaac tattatcaat acgagccctg gattgataca aaacaaaaaa cccatacata
     4981 tttcttcagc gacagaatga tctatcgtcc cggacaaacg atccacttta aaggaattcg
     5041 tttacatacg gatgccaatg gagaaaacgc gaaaattggt ccgaacgaaa aagcaagtct
     5101 gatcttctac gataacaact accaaaaaat cgcaagtctt gatttacgca gcaacgaata
     5161 cggaaccttc tccggatcgt ttaccgcacc ttcgggagta ctaaccggac aaatgcacat
     5221 taccgatgga tacggaaccc attacttctc cgtagaagaa tataaacgtc cgaaattcga
     5281 agccgatttt gaaccggtag aaggcgctta taaactcgga caaaaaatta aagtaaccgg
     5341 aaccgcaaaa gcttacgccg gatcaaatat tgatggcgcc aaggtaagct accgtgtaag
     5401 cagaacaacc tatttccctt acagatggtt ttactggtat tacgaccgcg atttaagcta
     5461 caaccaaatg gaaattgcat ttggtgaaac caccactaag gaagacggaa catttgaaat
     5521 cgaatttgaa gccattgctg atgaaagtgt ttcaaaaacc tataagcctg cattcaattt
     5581 tacggtatca gccgacatca cggatattaa cggtgaaaca cgttcggcca gcaccgttgt
     5641 aaatgttggg tacacggcaa tgacactcag tacttccatt tatggcgaaa tggacgtgag
     5701 cagtaaaacc ccggtatacc tctacgcaaa caatcttaac ggatcaccta ttgatgctaa
     5761 tggtagcgtt agtattcata aactcaaaca acccgacaga gtgctgcaac agcgtcgatt
     5821 gcaaagaccc gatcgtcacc aactcagtgc ggatgaatac aaaaaaactt tccccaatga
     5881 gctttatgca gatgagttaa atatttacaa ttgggccaga gagaaaaaag ttcacgagat
     5941 gcgtttcaac accaagacgc tcgactctat aattttaaag gaccttaata gttgggagcc
     6001 cggccattat ttgatcgaaa caattgcaaa agatccttat gacgaagagg taaaggacat
     6061 cacctatttt acggtgaccg acaacaaaag caccaaaatg tttttcagaa atgaattgaa
     6121 aataaatgct gatccctaca aacaatacga gcccggagag aaggcaatca ttctgctttc
     6181 caccaagtgg aaagacgtta ctgttctaat ggaaattgaa gagaaaaata aaattgtatc
     6241 tcgtcagtgg atcaggctaa gtgacgaaca gaaaaccatc agtattccca ttctcgataa
     6301 acaccgcggt aatttcgctg ttcatttcag cactgttcgt aacaaccggt tccacaacca
     6361 atctgttgtt atcaatgttc cttataccaa taaagaactc gacatcgaat ttgaaacgtt
     6421 caggaataaa atacttcccg gaagcgaaga agaatggaaa ttgaaaataa aaggtaaaaa
     6481 aggcgaaaaa ctcgctgcag aaatgttgct caccatgtac gatgcttcat tggatgcctt
     6541 tgcttcgaat gggttctatt ttcatccttt caattattat tacgcacagc actattggtc
     6601 ggcaggcgat ggatttgcct cgagaagtgg tcagcaatat ggtaaagact ggacacgcta
     6661 ccactccgca agggggagga attttgaccg catcaactgg tttggctatg aaacctattc
     6721 ttattacgga tacggttaca acaagcggta ttatatgcgc ggcggttacg acagagaaag
     6781 agtctctgca gtaacagaaa cggctgctac aggtgcgctt atggaagaac aaaaaaatgg
     6841 agaatcaatt agttttcctg cagcggctaa acaaacatat gcttggtcag gtgataatgt
     6901 tgatgatctt tcagtagcag aaaaagataa agacgggaaa aaaagcagca gagacgaatc
     6961 aggaaacaca cgtaatcccg aaggcaaagt gcaggttcga acaaacttca gcgagaccgc
     7021 atttttcttt cctcaattac aaacagatgc agagggtaag attgtgttca aattcaccgc
     7081 gccggaatca ctcaccaaat ggaaactgct tggcctggcg cacaccaaag aattgcaaac
     7141 cggaaccata gagaaagaag taattaccca aaaagaacta atggtgatgc ccaatgcgcc
     7201 ccgtttcttc cgggagggag accgcataac atttaccacc aagatcagca acctatccga
     7261 aaaagatctt gacggaacca ttaccctcga gctgatcgat ccgcttaccc aaaaaaccgt
     7321 gaacgcattg tttgctcaca aaggagaaca gatcaaattc actgtaaaaa aaggcgcaag
     7381 tacttcggca tcctggacat ttagtatccc cgaaaatctg ggcgcggttt cgtatcgtct
     7441 cgttgcgcgt tccggctcgt tttccgacgg cgaagaaatg gctgttccta ttttaaccaa
     7501 ccgaatgatg gttacggaaa gtatgccact acctatccgc aaggcaggaa caaaacaatt
     7561 caattttacc aaactcagca acagcaacaa atcatcaacg ctgcgtcacc ataaacttac
     7621 cttggaattc actaataatc ctgcctggta tgccattcaa gccatgcctt acatgatgga
     7681 atatccgtac gaatgtgccg aacaggtttt tacccgcttt tacgccaatt ccattgcatc
     7741 acacatcacc aattcgaatc caaaaataaa agcggtattc gaagactgga aacgcagttc
     7801 accggaagcg ttcttatcca atctggaaaa aaaccaggaa ctgaaatcac taatgctgga
     7861 agaaacccca tgggtcctcg aagcaaaaga cgaaagcgag cgtaaaaaac gtgttgctgt
     7921 gttattcgat cttaaccgca tgggcaatga acttagcagg gcacttggca agctccgcaa
     7981 tatgcaggta agcaacggtg gatggccgtg gttccctgga atgcccgaaa gccggtacat
     8041 cacccaacac atcatcaccg gaatgggaca tctggatcat ctgggagtta aaaatattcg
     8101 cgaagaacat tctacctggc aaatggtgca aaatggtgtg cgttatctgg atagcagggt
     8161 cattgaagat tacaactaca tcaaacgcca ttatcccaat tacaaaaaag aaaaacatat
     8221 cggttacgat gcaatacagt atctctatgc gcgcagttat ttcaaagatt tgtcgatgga
     8281 cgataacctg aaagaagcat tgctttatta ccaggatcag gcgaagaaat actggctcga
     8341 ttttaatctt tatgctcaag gaatgctagg tctcgcggct catcgtttta ccatgaaaga
     8401 actggcaggc gatatcgtaa aatcaatccg tgaaaaagcc attcgtcacg acgaattagg
     8461 aatgtattgg aaagacaatg ttgttggtta tttctggtac caggcaccca ttgaaaccca
     8521 cgcgctgatg atcgagatgt ttgacgaggt tgccaatgat caggaaacag ttgaagagct
     8581 gaaagtatgg ttgcttaaaa acaaacaaac cagcgactgg aaaaccacca aagcaacagc
     8641 agaagcctgt tatgcgctgc tcctgaaagg aacagacctc ctctctaatg atgaaatggt
     8701 ggagatccgc atgggcaatg aaatcgtaga tcctgccaag atgggggcaa aagtggaagc
     8761 cggaaccgga tacttcaaaa cctcctggag cgctgaaaaa atcactccgg aaatgggtaa
     8821 aatcacactc agcaagaaaa aagacggagt agcctggggc gcagtatact ggcagtattt
     8881 cgaacaactc gataaaatta ctccacacga aacacctctg aaactcaaca aaaaactctt
     8941 cctggttaag aaaaccgaaa ccggacaaca aatggttcct gtggatgaaa aaacatctct
     9001 tgagccggga tcgcgtgtaa gggtaaaaat tgagctgcgg gtagatcgtg ccatggagta
     9061 tgtacatatg aaagatatgc gcgcatcctg cttcgagcct gtaaacgtgt tttcgcaata
     9121 caaatggcag gacggactgg gctattacca aagcacccgc gatgcagcca ccaacttctt
     9181 ctttgagtac cttcctaaag gaacccatgt attcgaatac gatctgctgg tgtcgcatct
     9241 aggcgatttt tctaacggta ttaccaccat tcaatgcatg tatgcccccg agttcacatc
     9301 acactcagaa ggtatacggg tacaggtaaa aggacaataa gttgcgatta ttgcgggatg
     9361 tcttatcttt ggagaaaacc taagcaatga aaaagtatct tttccttctg gctcttcctg
     9421 taagcttgtt tttatcctct tgcggaggcg aaacaaaaac cgacgagaat aacgacgaca
     9481 gcacggtagt gtcggatatc gactataccg gaatgagcga attcgacctc gaaccacacg
     9541 gcttgatggc atcaatgatg gtgaccgaca tcatctctga aaacggaagt ccatttcctg
     9601 taacagttgt tcgcgacacc aatatgctta cctgggacat caaagttgga aaagaaggca
     9661 atgaagagtt ctacctcatt attgaggaga cggatggcga aggaaactac atcaagcgcg
     9721 agaaagaccg tttggcttct gatgatgttt ttaaagaaga atacctgacc gacgaaaatg
     9781 acctgctgct ctataaggct tcactccctg aaggcactgg tcagcgcgat tactaccacg
     9841 tatttggtct tgttaaaatt ggaaagcttg agtacatcgt tcactcctac gcaatgggag
     9901 attacagcga agtacaggca cgcgatatgc tcaaaggaat tcaatccctc agagagccat
     9961 cagcgatttg attttataag tgaatttaat tgaaagggct gcaatttgtg gccctttttt
    10021 tgtcctagcc ttagcctcaa cgttcaggta tccccgatcc ggtttacccc aggtaattca
    10081 acccctctta ttaccgtatc tgcacttcct taaagtagag aaacaacggt ttatttaaag
    10141 ttcaatgctt gttccgctcc tccacaaaac aaaaaagcct gtcgggttcg acaggctttt
    10201 tttttatagt agatcaattt ttcttttcgg ctggcttttc agcaaccgga gctttcagat
    10261 aaatatctga gagatcgaaa tagctcatcg gattattgtg gaaattatga acgctcgaat
    10321 gaagcaggcg ataattctca acataccaaa tgatgattac agggcaatcc tgcatcataa
    10381 tgctttctgc cttcgcaaag tttgcataac gctcctcctc tgtttttgcg gccatcccca
    10441 attcataata cttgtcgaac tctgcgctct tgtaacgcat tgtattgggg tgtgaaggaa
    10501 gatccagtgt tgcaggcaca ttctttccgt aggctatgga aaggaacgat tcaggagagg
    10561 gataatctgc cacccatgca gagcggtata tgtcggcacg accatattgg gagtcttcaa
    10621 tcttttgcgc aagcgatacc tgctcaagct cgatgttgat tcctaataca tctctccatt
    10681 gacgcataac ttcactagcc acttttgtgt tgcgtgagcc gccataattt acaagtaact
    10741 taagtgtagg aaaacctttt ccaccagggt aacccgcctg ggccattagt tttttagcca
    10801 tttcaggatt ataggtatat ccacgggtta ttgaagtgtc gtacttggaa aactgataaa
    10861 gtggcggaac cagaccgtag ttacccgttt ggttctggcg tgcaccgttg gcagcagcct
    10921 gaccattcag aacgttctcc atgatcttgg agcgatcgat agccatagac aatgctcttc
    10981 ttacacgaac atctttaaac tgcggacgag taagattaag ttcataaaac tgggttgaca
    11041 tttccggttc gcgaagcaaa atatactttg gtggcttatt ggtgaaatcc ggaatgtttt
    11101 cctgaaccac ttcagaaatt ttctctgcag gcagtccgta aatcatactc agttcacctt
    11161 cgcggaacat atcaagctcc tcagtgtctt catttacaaa atagatatgt actgtatcaa
    11221 ggtagggtag ctgattaccg tgtttgtcta cacgataata gttttcattt ctaaccagaa
    11281 tcaactcact ttttgcatca ccaccagtac tgaatttaaa tgcccctgtt ccaattttta
    11341 agtctttacc gtacttctca tacccttctt taggaataac tgaagcaaca ggattagcca
    11401 aaatgtaaag gaaggctgaa gatggagcga tagtgcggat ctttactgta taatcatcaa
    11461 caacctcaac gccgctcact tcttttgctt ctccattata gaaagcctgc gctccttcga
    11521 gacgctcttt aaaattaagt tcgtaggatt gatcgctaat gccttttgtg caggccagtt
    11581 ccagcgagaa tttaatgtca gtagctgtca ccaaacggcc ttttccatca gcaaaacagg
    11641 ggtcgtcatg gaaacggaca tccttgcgaa gatggaacac atattctttc tggtcatcgc
    11701 ttacttccca actttcggca atactgggtt gaatggacag gtctttctga ctcattctca
    11761 ccaatccttc aaaaatctgc caggcaatgt gtgcagagcc ggcatccttg atttcgtagg
    11821 ggaacagcgt gttatacttc tcgttttccg caaggttgaa ggagcctcct aaggcaacat
    11881 cgcctttggc gatacgagtt ggtttttccg tttcgttaga tcctgattct gaaccacctc
    11941 cgcaggagct cagcaaaaag gagcctgcga tggtgtaggc aagcaagcgt ttcatgatgt
    12001 tgcagttttt gggaatactc acaataataa cctttaatag gcaaaaatgc aaaataatct
    12061 gttatcaacc aatgagttca ggccaagaaa aggtttatcg acgaaaaaag ggggttaaac
    12121 ggcaaaattt tagtgttttc tttttgaatt gattaaaatt gaagttaata ttgcgcctta
    12181 atgaaaa
//
LOCUS       DAOMYG010000026        12094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1751_length_12094_cov_10.177756, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000026 DAOMYG010000000
VERSION     DAOMYG010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 12094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12094
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1751_length_12094_cov_10.177756"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PK610_01655"
     CDS             complement(<1..480)
                     /locus_tag="PK610_01655"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRJ34657.1"
                     /translation="MYQDLEWSIPVSGPELFITFDDGPIPEITPRVLEILKAYNAKAT
                     FFCIGENVRKHPEIYRQILEEGHRTGNHTQHHINGWKTNKINYLRQSLLCSHYVQSDL
                     FRPPYGRISRAQAKALKSRFRIIMWDVLSMDYDKRISREKCLDNVVKNAGPGSIIVFH
                     "
     gene            complement(518..4078)
                     /locus_tag="PK610_01660"
     CDS             complement(518..4078)
                     /locus_tag="PK610_01660"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRJ34658.1"
                     /translation="MNYKLLNNVLGWLAFAIATWVYFATAEETVSLWDCGEYISTANT
                     LQVGHPPGAPLFNMLGRLFSAFSEPHEAAHAINRMSALCSSFSILFLFWTITFVAKKL
                     ATIKGEELSDAKIIAILGSGFIGAMAYTFSDSFWFSAVEGEVYAMSSFFTAIVIWAIF
                     KWDAVADEPGADRWIIFIMFLIGLSVGVHLLNLLAIPALGFVYYFRRYKHSTKGFIFA
                     GIGSIVILGIIQAIIIPGMVSMPAFFERTFSNMGMPFNFGAWFFFIALIGLLVYGIMY
                     THKKKKYFLNLALNSFAVVVIGYSCFGMIMIRSQANTPIDENNPENFVTLLSYLNREQ
                     YGSWPIVSGPYWNSPITGQKDGNPVYMRGYAIKRGERLVKGFRTEQEAKDYIAKKGLA
                     GVTVNEEYFIADDRKGTEYTYSSEHTTVLPRMFSRDYRHVIGYKNWSKYDGSGKVKVQ
                     GRDGQTEALPTFGNNISFLMNYQLGWMYWRYFMWNFSGRQNDEQGTDGTPLEGNFITG
                     VKFIDNELIGNQDNLPEHMKSNPSHNRFYLLPLILGLIGFFWQLVRDPKNWFVVFLLF
                     FFTGIAIILYLNPKPYEPRERDYAYVGSFYAFAIWIGIGVYALFDIAKNLKLRDFAFF
                     AGGTTAFTLILYMFEGKDGHAFSYIMFYIALVSFAAMAIMIVMNMFGKNDMLTAATSI
                     LLAVPAPYLMAKEGWDDHNRSNRSTALDLAWNYLQSCEPNAILFTHGDNDTFPLWYAQ
                     EVEKIRRDVRVVNLSLLGTDWYIDQMVRRAYESAPVPFTFPEHIYRQGGAIDQAFVNT
                     RNDKFEDLVFMMDSLRNDDNHVQLDAGSSRRFAVLNTPKLYLKVDKEAVKANKVVGEH
                     DYNKIVDTVKFIVPKPQGYMFKNDVMILDLVAHNNWERPIYFAGTADAETYMGLGDYF
                     LMTGLNYKFVPMKNQNPNPREFGKIDTDTMYKMFMEVYRWGNMNGEGVYVDYYTRRLT
                     NNYRLQFLNLAEALNSEAREAQGKLSYYQMQIRVTEDSLKRGSNSAMEQRLVNLKAQE
                     KKNQEIIEAKHNRASKVLRRSLEVMPEKNVPFDRIVPSYVPIALEAGDDSLATALVER
                     LVEVNDANLRYYFNAGERFSINMMDDIDVSRRILMMMYQICTQYKKAELLAKIEPVLN
                     AQDAAFASWMNRILQYDRRMGMNNLMRSFPNEMQQLFQMQ"
     gene            4220..4290
                     /locus_tag="PK610_01665"
     tRNA            4220..4290
                     /locus_tag="PK610_01665"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4252..4254,aa:Gln,seq:ttg)
     gene            4714..7560
                     /locus_tag="PK610_01670"
     CDS             4714..7560
                     /locus_tag="PK610_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRJ34659.1"
                     /translation="MTKNKRNLWMIAGLLATLILTYVLWPVQKEPDVLPPVFKEAAFL
                     ETDPAWADSVLNTLSPEEQLAQLFFPVLPLSNLKDDDSIVFEKGMPEPGGIVFSGHFP
                     ERQLRISASVLRQQKVPLLLSHESPFYAASPNKIITDQGAIFGVGDTSFTNWFIRQSI
                     ELFPAVGCDLLFFPQSSSVHFSLSEQLLQLYLHRTGGKNLLGVAGKLDRIFDPVKDSL
                     INADSLLRPWRTLCDSGLAVIEADTALLRSRHLKKGSIAIRDFCEDHLDFKGLLLARF
                     PEGEQDPALLLRTWFAEGMEIIVTGTDHFKVFDAARNMLKRGEISEQELQRRTRKILL
                     AKSWIRKQNFRTSEDLISPRKMQHAFGKVFAQQLEERSVVLLNERSEEFPLRFHQEKR
                     MMVLAIGEENMPFRKEIQRYADVNWIPIHHFNELSAQKPDNLKRFSPIVLFLNNIAPT
                     AEEFANCKSVFSRPDLIVVHSGQTTDLPLLQSCAVVVHSDRVDAPAQEALARALLGTE
                     AISGRLTRHTGSYKAGHGKKQNATRLKFARPEEIGVANDSLYRIDAIMDEAIRNHALP
                     GGQVLLAYDSKIIFHKSFGHHTYDRKIVVDQQHRYDMASVTKVAATTMMAMHLYEQGK
                     FLLDDSLKKHLPDTLDKFLPSGSSLKHVTFRQLLVHASGLPPGQNIFRFMRFDEERSP
                     YDLYFCDERSKQFSLVVGDSFYLDKDCLDTLWLDLNKIWLDPAKPYSYSDANMNLLYT
                     MLAAKLKKGVRWERAVDSLFYQPLGMKRTGYIPLQRGIKKEEIAPTENDRYWRRQLIQ
                     GHVHDPTAALYGGVAGNAGLFSNAYDMAVLFQMLLNKGEYGGRRYLKRETVELFTSRQ
                     NGSHRGLGFNMQVSGSTYGCSPYASASTYGHTGFTGTAVWVDPERKFIYVFISNRVHP
                     DPENKKIIKLGITKRIHNVLYEQLGFGVSEPVLL"
     gene            7645..9177
                     /locus_tag="PK610_01675"
     CDS             7645..9177
                     /locus_tag="PK610_01675"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRJ34660.1"
                     /translation="MAYVGTILSELLKINKRIRKRLTKYDAGLQEKTLRKLLSKARST
                     RMGKRYGFKEMLLVEDVYGEFSRRVPIYDYDKLHREFWVHTLKGKRNITWPGKIKYFA
                     LTSGTSGSPSKRVPVSLRQIKAVRKASVRQMLSITDFNLPAEFYEKGVLMVGGTTTLR
                     KIDAGYEGDLSGILQNNIPRWFDRFYKPGKKMAQILDWNDKLDAMAMEAPKWDIGIIC
                     GVPAWIQMLFDRIKKFHNVKHIHEIWPNLSIYVHGGVSFTPYRESFRSYLGRDIHYLD
                     TYLASEGFLALQLAEHQKGMQLILDNWVFFEFVPFNDSNFDADGNIRENPEVLTIEKV
                     EEDVDYAILISTCSGAWRYLIGDTVRFRDKKNFEMIISGRTKHFLSLCGEHLSVDNMN
                     MGMRKAMDAFGVNVNEYTVAGEPYEGSFRHHWYIGVDKDVDGEAFKRKLDEALCELND
                     DYTTERKHALKDPILEVIPIERFYRYMELKGKAGGQNKFPRVIRNSQFEEWKRFLEKG
                     EL"
     gene            9300..10493
                     /locus_tag="PK610_01680"
     CDS             9300..10493
                     /locus_tag="PK610_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ34661.1"
                     /translation="MNELLDRSNALILGVSLDFQRSLLEEVRWNNRLIGIKGARGTGK
                     TTLLLQWLKLRNQPADKAAYFTLDDLYFTTHSLRELAREFYLNGGRILVLDEVHRYSN
                     WSLEIKNLYDLYPDLRIVFTGSSVVDITRQEADLSRRALMYELPGLSYREFLAVSGAG
                     VFPVLDLGQLCADASIYRSAFPTFFHPLEAFKRYLRIGYYPIFLEDEMVVQQRIRQMV
                     RVIIENDMSAVKDFDLRNSGKLLQLLYILSQQVPFKPNLTDLAAKTGIHRNTLNTYLY
                     YLEQARLIHLLRPAGITDGVLQKPEKIYLDNPSVLFALAESEPNKGNLRETFFLSQLK
                     TNHQVRAVAKGDFLVDNKFTFEIGGKSKTEKQVRGLPSAFIVKDDIEWPIGKAIPLWM
                     FGMLY"
     gene            complement(10494..11936)
                     /locus_tag="PK610_01685"
     CDS             complement(10494..11936)
                     /locus_tag="PK610_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ34662.1"
                     /translation="MLLRNLLVCFFCGTAVIFSSATHAQIWLSEPFTYHTEAIPNGDT
                     LIYYGYSGDSYRLTSFEWSTGQYHHAWLSDSVQKLFPLHLLPNGDVIAAYDRFYPDTF
                     FYESVFKKFSGGSLTNTYIIADPANTCRILAIAYTDTNTLVYHSACGGQNGYVIALDS
                     NFGNPDTLVADLTANFSLQMSRQLALRAPDEIQITGILQPYSTSDYSLHRYSVNTNIP
                     LVIEMTYPSWIQQIDQNTEPNLYNIKNTLGYKNFDVFGFNAQYFINFFNQEMIGVLQL
                     DAAGFPQRSYTYRDPAGHDYIFADLCKTSDGGFLLLANRGENSSFYSFPPGEGILLQK
                     LNDTLGLEWSRFFPGPEGNSFFSGFFIFPFQNGYCIQGWGTPDRNCQSFFLLLDENGI
                     ISSIDETILSENLLLFPNPNNGSFQLTWPQEKILSVEIYTLSGARVHQQNAFGNESLS
                     IDLNLSNGVYFIHAVNANGNRVVKKVVIAK"
BASE COUNT         3322 a         2518 c         2823 g         3431 t
ORIGIN      
        1 gtgaaaaaca atgatggagc cgggacctgc attttttacc acgttatcca ggcatttttc
       61 gcggctgatc cttttgtcgt aatccatact cagcacatcc cacataataa ttctgaaacg
      121 gcttttcagt gccttagcct gagcacggga gattctgccg tatggggggc gaaagagatc
      181 ggattgtaca tagtgagagc agagcaggct ttggcggaga tagttgatct tgtttgtttt
      241 ccagccgttg atgtggtgct gggtatgatt accggtgcgg tgaccctctt ccagtatttg
      301 gcggtagatt tccgggtgtt ttcgcacatt ttcgccaata cagaaaaagg tagcttttgc
      361 attgtatgct ttgagaatct caagaacacg aggtgttatt tccggaatag gaccatcatc
      421 gaaggtgata aaaagctcag gacctgaaac ggggatactc cattccaggt cctgatacaa
      481 tgcctttaca atatgtggaa ttttagctct gaccagacta ttgcatttgg aacagttgct
      541 gcatttcgtt cgggaagctt ctcatgaggt tgttcattcc catgcgacga tcgtactgaa
      601 ggattctgtt catccaggat gcaaatgctg cgtcttgtgc gttgagaaca ggttcaattt
      661 tagccagcaa ttccgctttc ttgtactgtg tacagatctg atacatcatc atcagaatgc
      721 ggcgcgaaac atcaatatca tccatcatgt tgatcgagaa acgttctcct gcattgaaat
      781 aataacgaag gttggcgtcg tttacctcaa ccaatcgttc taccagagca gttgcaagtg
      841 aatcgtcgcc cgcttctagc gcgattggca cgtaagaagg aacgattctg tcgaacggaa
      901 cattcttttc aggcataact tccaaggaac gacgaagtac tttggatgcg cggttgtgtt
      961 tcgcttcaat gatttcctgg tttttctttt cctgagcttt caggttgaca agacgctgct
     1021 ccattgcact gttgcttcca cgcttgaggc tgtcttccgt aacacgaatt tgcatctggt
     1081 aatagctgag tttgccttgt gcttcgcgtg cttcgctatt gagtgcttcg gcaagattca
     1141 ggaattgcag acggtagtta ttggttaagc gacgtgtgta atagtcaacg taaacacctt
     1201 ctccgttcat gttcccccag cggtatacct ccatgaacat tttatacatg gtatcagtat
     1261 cgattttacc aaactctctt gggttgggat tctgattctt catggggaca aatttgtaat
     1321 tgagtcctgt catgaggaaa taatcaccta atcccatgta ggtttctgca tcggcagttc
     1381 ctgcaaaata gatcggacgc tcccaattgt tgtgggcaac cagatcgaga atcattacat
     1441 cgttcttgaa catatatccc tgtggttttg ggacaatgaa tttaacggtg tccacaattt
     1501 tgttgtaatc atgctcaccc acaactttgt ttgctttaac agcttcctta tctactttca
     1561 ggtaaagttt aggggtgtta agcacagcaa accggcgact gctaccggca tcaagttgta
     1621 catggttatc gtcgttgcgc agcgaatcca tcatgaagac caggtcttcg aatttatcat
     1681 tgcgtgtatt tacgaaagcc tgatcaattg caccaccctg acggtagatg tgttcgggaa
     1741 aagtaaatgg aaccggagca ctttcgtatg cgcggcgcac catctgatcg atgtaccaat
     1801 cggtacctaa aagagagagg ttgacaacac gaacatcgcg acgaattttt tcaacctcct
     1861 gtgcatacca caaggggaaa gtatcgttat cgccgtgggt aaagaggatg gcattcggct
     1921 cacaactttg caggtaattc catgccagat cgagagctgt acttctgttg cttctgttgt
     1981 ggtcgtccca accttctttc gccatcaggt aaggtgcagg tactgcaagt aaaatggatg
     2041 ttgcggcagt aagcatatcg ttctttccga acatattcat gacaatcatg atggccattg
     2101 ctgcaaagga aaccaatgcg atatagaaca tgatgtatga aaaggcgtga ccatcttttc
     2161 cttcgaacat gtaaaggatc aatgtaaatg cagttgttcc tcctgcaaag aatgcgaagt
     2221 cgcgcagctt gaggtttttt gctatatcaa agagtgcata aacaccaata ccgatccaga
     2281 ttgcaaaagc ataaaacgat cctacataag cgtaatcgcg ctcacgtggt tcataaggtt
     2341 ttggattgag gtataggata attgcgatac ctgtaaagaa gaaaagcagg aaaacaacaa
     2401 accagttttt cgggtcgcga acaagttgcc agaagaaacc gatcaaacca agaattaatg
     2461 gaagaaggta aaaacggttg tgcgacggat tgctcttcat gtgttcggga aggttgtcct
     2521 gattcccgat cagttcatta tcgatgaact tcactcccgt aatgaagttc ccttcgagcg
     2581 gagtgccatc ggttccctgc tcatcgttct gtcgtccgga aaaattccac atgaagtatc
     2641 tccaatacat ccagcctaac tggtaattca taaggaagct gatgttgttg ccaaaggtgg
     2701 gaagtgcttc ggtctgacca tcgcgcccct gtactttaac ttttccgctg ccatcgtatt
     2761 tcgaccagtt tttgtaaccg attacgtgac ggtaatcgcg gctgaacata cgaggcagta
     2821 ctgtggtgtg ttcggatgaa taagtatatt ctgttccctt gcggtcatcg gcaataaagt
     2881 actcttcgtt aacagttaca ccggcaagtc ctttttttgc gatgtaatct ttcgcttctt
     2941 gttctgtacg gaatcctttt accaaacgct caccgcgctt aatggcataa ccacgcatgt
     3001 aaacgggatt accgtctttt tgtccggtaa tgggagagtt ccaatagggc ccacttacaa
     3061 tcggccagct tccgtactgt tcgcggttga ggtaagagag aagcgttaca aagttttcgg
     3121 gattgttttc atcaattggt gtattggcct gcgagcggat catgatcatt ccaaagcagg
     3181 aatacccgat tactaccact gcaaaactgt tcagggcaag attgaggaaa tattttttct
     3241 ttttgtgtgt atacataata ccatacacca gtaatccgat cagcgcaatg aaaaagaacc
     3301 atgcaccgaa attaaaaggc atacccatat tgctgaaagt acgctcaaag aaagcaggca
     3361 tactcaccat acccggaata ataatggcct gaatgattcc cagaataacg atggaaccga
     3421 ttccggcaaa aatgaatcct tttgttgagt gtttgtagcg gcggaagtaa taaacaaaac
     3481 caagtgcagg gattgcaagc aagttcaaca agtgaacacc aactgataat ccgatcagga
     3541 acatgataaa aataatccag cggtctgccc ccggctcatc ggcaacagca tcccatttaa
     3601 agatggccca gataacaatg gcggtaaaga aagaagacat tgcataaacc tcaccttcta
     3661 cagcagagaa ccagaaagag tcggagaagg tataggccat agctccgata aatccactac
     3721 ccagaatggc gatgattttc gcatcgctaa gctcctctcc ttttatagtc gccagttttt
     3781 tggcaacaaa ggtgatggtc cagaatagga ataaaattga aaaggaggaa cacaaggccg
     3841 acattctgtt aatggcgtgt gcggcttcat gtggttcgct gaaggcagaa aagagtcggc
     3901 caagcatgtt aaacaaaggc gctcccggag ggtgtcctac ctgaagggtg ttcgcggttg
     3961 agatatattc gccacaatcc cagagactta cagtttcttc ggcagttgcg aaatagaccc
     4021 aggttgcgat ggcaaaggcc agccagccca gtacgttgtt taacagtttg taattcatgc
     4081 gcgttataaa gtgatgcgaa aataggaaaa taaatccccc ttatatggtg cttaacggat
     4141 tttaacggct tggccgaaat agagcctctt cacttttttt tgtgaggtta tggcctttta
     4201 ttacttttgc agccgcgatt gcccgatcgt ctaatggcag gacttcagat tttggttctg
     4261 actgtgttgg ttcgaatcca gctcgggcaa cttaaaagca aagaaaggcc acgaatttat
     4321 cgtggccttt tttatttgtt tcgttaacat aagcatttga taattaaatt gtttattgat
     4381 tgaatcgcaa ttgctacaat ttgctctgta ctttttgctt gaccaaaaag taccaaaaac
     4441 tcaaggcttc aaatcaaccc tgcgaaaaaa cttgaatttg ccaacttcgg ccgggtgatc
     4501 ccttccgatc ccggaagtgc ttcccggtct acgtaagcaa attcagcgtt ttttcttgtt
     4561 ttgatttgag gccgacggtt tatttaagtt ctacgaatac tagggttcgt aaatactttt
     4621 agttttccct ggaaaaaaac catagggaat ttcttgttta tatgtttcat ctacttgcta
     4681 atgaatgatt agggaagaat tcgatatttg cgcatgacaa aaaacaagag gaatttatgg
     4741 atgatcgcag gtttgcttgc gaccttgatc cttacctatg tcctctggcc ggtgcaaaag
     4801 gagccggatg ttttgcctcc tgtatttaag gaagcagcat ttcttgagac tgatcctgca
     4861 tgggccgatt ctgtattgaa taccctgagt ccggaagaac aacttgccca attgtttttt
     4921 ccggttttgc ctctgtcaaa cctaaaagat gatgattcga tcgtttttga aaagggaatg
     4981 cctgaacccg ggggcatcgt tttttccggt cattttcccg aacgtcagct gcgcatttct
     5041 gcatcggtcc ttcggcaaca aaaggtgcct ttacttttga gtcatgagtc gcctttttat
     5101 gctgcatctc ccaataaaat cataaccgac cagggagcga tatttggtgt gggagatact
     5161 tcttttacga actggtttat ccgacagagc atcgaacttt ttcctgctgt tggttgcgat
     5221 cttttgtttt tcccacaatc atccagcgtt catttttcat taagcgagca gttattgcaa
     5281 ctgtacctac atcgaaccgg tggaaaaaac cttcttggtg ttgcaggaaa actcgatcgc
     5341 atttttgatc ctgtaaaaga ttctctcatc aatgcagata gtctattgcg tccctggcgc
     5401 acattgtgcg atagcgggct tgcagttatt gaagcagata ctgcattgtt gagaagccgt
     5461 catttgaaaa aaggatccat cgctattcgt gatttttgcg aagatcatct ggattttaaa
     5521 ggcttgttac tggcgcgttt tcctgagggc gagcaggatc ctgcgctatt gttgcgcacc
     5581 tggtttgcgg agggaatgga gatcattgtt accggcaccg atcatttcaa ggtgttcgat
     5641 gcagcacgca acatgttgaa gcgtggggaa atttcggagc aggaattaca gcgccggacg
     5701 cgaaaaattc ttttggcaaa atcgtggatt cggaagcaga attttcggac aagcgaagac
     5761 ttgattagtc cgcggaaaat gcaacatgcc ttcggaaaag tttttgcaca acaacttgaa
     5821 gaacgttcgg tggttttact caacgaacgc agtgaagagt tccctcttcg ttttcatcag
     5881 gaaaaaagaa tgatggtact tgcaattggt gaggaaaaca tgccattccg caaagaaatt
     5941 caacggtatg cagatgtgaa ttggataccc atccatcact ttaatgagtt gtcggcacaa
     6001 aaaccggata acctgaaacg tttttctcct attgttttat tcctgaacaa tattgcccca
     6061 actgccgagg agtttgcaaa ttgtaaatct gttttttcac gtcccgatct tatcgtcgtg
     6121 cattccggac aaacaacaga tctacccttg ctgcagtctt gtgctgtggt agtacacagc
     6181 gatcgtgttg atgcgcccgc tcaggaggct ttggcacggg ctttattggg cactgaagcg
     6241 atttcgggaa gattaacaag gcataccggt tcctacaaag ccggacatgg taaaaagcaa
     6301 aacgcaacac gtctgaaatt tgcgcgtcct gaagaaatag gtgtagccaa cgattcgctt
     6361 tatcgcattg atgctattat ggatgaagcg attcgtaatc atgcattgcc cggcggacag
     6421 gtgttgttgg cctatgacag taagatcatt tttcacaaaa gttttggtca tcatacctac
     6481 gatcggaaaa ttgtggtcga ccagcaacac cgttacgaca tggccagtgt gaccaaagta
     6541 gcggcaacca caatgatggc catgcatctg tatgaacagg gaaaattttt gttggacgac
     6601 tcgctaaaaa agcacttgcc cgatacactg gataaatttc tcccttcggg tagcagttta
     6661 aagcatgtta ctttccggca attgttggtt catgcaagcg gattaccacc cggtcagaat
     6721 attttccgct tcatgcgttt tgatgaagaa cgttcgccct acgatctcta tttctgcgat
     6781 gagcgcagca agcaattctc gctggtggtt ggcgattcgt tttacctcga caaagattgt
     6841 ttggatacgc tgtggcttga cctcaacaag atctggcttg atccggcaaa accctattcc
     6901 tattcggatg ccaacatgaa tttattgtac acaatgctag cggctaaact gaaaaaggga
     6961 gtgcgttggg agagggccgt tgattcattg ttttatcagc cactgggcat gaaacgaacc
     7021 ggttatattc ccttgcaaag aggaatcaaa aaagaagaaa ttgctcctac tgaaaatgat
     7081 cgttactggc gcaggcaatt gattcaggga catgttcacg atcctacagc tgcattgtat
     7141 ggtggtgtag ccggaaatgc aggattgttt agcaatgcct atgatatggc agtgttgttt
     7201 cagatgttgc tgaataaagg agagtatgga ggaagacgat acctgaagcg ggaaacagta
     7261 gaattgttca cttcacgaca aaacggatcg cacagaggct tgggctttaa tatgcaggtt
     7321 agcggaagta cctatggttg ttctccctat gcaagcgctt cgacatatgg tcataccgga
     7381 tttaccggca cagcagtatg ggttgatccg gagcgaaagt tcatttatgt gtttatttcc
     7441 aacagggtgc atcccgatcc cgaaaacaaa aagatcatca agctgggaat tacaaagcgc
     7501 attcataacg tgttgtatga gcaacttggt tttggtgtat cagagccagt tttgctgtag
     7561 aatacaggct attgtttaca aattctcgta ttgaccattc ctccgtttaa ggtctgagtt
     7621 catattcttt taattttgcg agcaatggcc tacgttggaa ccatattatc cgaactgctc
     7681 aagatcaaca agcgcatacg taaacgcctg acgaagtatg atgcaggttt acaggagaaa
     7741 acgcttcgaa aacttttatc gaaagcgcgc agcacacgta tgggtaaacg ttacgggttt
     7801 aaggaaatgt tgctggtaga agatgtgtac ggagaattca gccgacgtgt acccatttac
     7861 gattatgata aattgcaccg tgagttttgg gtgcatacac tcaagggaaa acggaacatc
     7921 acctggcccg gtaagatcaa atatttcgcg cttacttccg gcacatccgg atctccttcc
     7981 aagcgtgtac cggtcagcct gcgacagatc aaagcagtac gtaaggccag tgttcgtcag
     8041 atgctttcca ttaccgattt caatttacct gcggagtttt acgagaaagg tgttttgatg
     8101 gttggaggca ccaccacatt gcgaaaaatt gatgccggtt acgaagggga tctcagcggt
     8161 attttgcaga acaatattcc ccgctggttc gatcgttttt acaagcccgg caagaagatg
     8221 gcgcagatcc ttgactggaa cgacaaactg gatgcgatgg cgatggaagc gcctaaatgg
     8281 gacatcggta ttatttgcgg agttcctgcc tggatccaga tgttgtttga tcgcatcaag
     8341 aagtttcaca acgtaaaaca tatacacgaa atctggccta acctcagtat ttatgttcac
     8401 ggcggggttt catttacacc ttaccgcgaa tcgttccgca gttatctggg gcgcgatatt
     8461 cattaccttg atacttatct tgcttcagag ggatttcttg ctttacagtt ggcagagcac
     8521 caaaagggaa tgcaactcat tctggataac tgggtctttt ttgagtttgt tccatttaac
     8581 gattctaatt tcgacgccga tggaaacatc cgcgaaaatc cggaggtgct gacaattgaa
     8641 aaagtggagg aagatgtaga ttacgccatc cttatttcca cctgttccgg cgcatggcgt
     8701 tatctgattg gcgatactgt acgttttcgc gataagaaga attttgaaat gatcattagc
     8761 gggcggacca aacatttcct ttcactttgc ggtgagcatt tgtcggtcga caatatgaac
     8821 atggggatgc gtaaggcaat ggatgctttt ggggttaacg taaatgagta caccgtagcc
     8881 ggtgaacctt atgaaggaag tttccgtcac cattggtata ttggtgtaga taaagatgtg
     8941 gacggggaag catttaagcg caagctggac gaagcactct gtgaactcaa cgacgattac
     9001 accaccgaac gcaaacacgc gctaaaggat cctattctgg aagtcattcc tattgagcgt
     9061 ttttaccgct atatggaact caaaggcaaa gcgggcggac aaaataagtt tccccgcgtt
     9121 atccgaaatt cccagtttga ggaatggaag cgttttctgg aaaaaggaga gctgtaacca
     9181 atttattatc aaggctttgc tgtaagattt tagattttgc actttggaaa gtgcattttt
     9241 gattattttt gcactttaga aagtgcattc tgtggcaaaa ctaaattatt cttttgtata
     9301 tgaatgagtt actcgaccgt tccaatgcct taatactggg agtttctctt gattttcagc
     9361 gctcgttatt agaggaagta cgttggaaca accgcttgat tgggataaaa ggtgcaaggg
     9421 gaaccgggaa aacaacactt ctgttgcaat ggttaaaatt gagaaatcag cctgcagaca
     9481 aagccgctta ttttacactt gatgacttgt attttacaac acacagtctt cgtgaactgg
     9541 caagggaatt ttatttgaac ggggggagga tcttggtgct tgatgaagtg catcggtatt
     9601 caaactggtc gctggaaatc aaaaatctgt acgatctata tcctgatctc cgcattgtgt
     9661 tcacaggttc ttcggtggtg gatatcacgc ggcaggaagc agatttaagt cgacgcgcat
     9721 tgatgtatga attgccgggc ttgtcttacc gtgaattttt ggcagtttcc ggtgcgggtg
     9781 tttttcctgt tttggacttg ggccaactct gtgctgatgc atcaatttac cgatctgcat
     9841 ttccgacatt ttttcatccg cttgaggcat ttaaacgtta cctccgaata ggatattatc
     9901 ccatttttct ggaggatgaa atggtggttc agcaacgtat tcgacaaatg gtgcgggtga
     9961 ttattgagaa tgatatgtcg gctgtaaagg attttgatct gcgcaactca ggtaagcttt
    10021 tgcaattgtt gtacatcctt tcgcagcaag tcccttttaa acccaatctt accgatctgg
    10081 cagcaaagac cggaattcat cgcaacacat taaatacata cctgtattat ctcgaacagg
    10141 cgcgattgat tcacttgtta cgtcctgcgg gaataacaga cggagtcttg cagaagccgg
    10201 agaaaattta cctggataat ccatcggtac tattcgcttt ggcggaatca gaacctaata
    10261 aaggcaatct gcgtgagacg tttttccttt cacaacttaa aacaaaccac caggtaagag
    10321 cggttgccaa gggcgatttt ctggtggata acaagtttac ctttgaaata ggaggcaaat
    10381 ccaaaactga aaaacaggtt cgtggtcttc catccgcctt tatcgtgaag gatgatattg
    10441 agtggcccat cggtaaggcc ataccacttt ggatgttcgg aatgctgtat taattacttc
    10501 gcaattacta ctttcttcac cacccgattt ccatttgcat tcaccgcatg aatgaaatag
    10561 acgccgttac tcaggttgag atcaatagaa agactttcgt tgccgaatgc gttttgctga
    10621 tgtacacgcg cgccgctgag ggtgtaaatt tctacactta gaattttttc ctggggccag
    10681 gttagttgaa agcttccgtt gttggggttg gggaagagaa gaaggttttc tgacagaata
    10741 gtttcatcaa tcgaagaaat gatcccgttt tcatcaagga gaaggaaaaa gctttgacaa
    10801 tttctatccg gtgttcccca gccttggatg cagtaaccgt tttgaaatgg aaaaataaaa
    10861 aatccggaaa aaaaggagtt tccttcagga ccaggaaaaa aacgagacca ctccaaaccc
    10921 agtgtatcgt tgagcttttg caaaagaatc ccttcgcccg gaggaaaaga ataaaaggaa
    10981 gaattctctc cacgattggc caataataaa aatcctccat cagaagtttt gcataaatcg
    11041 gcgaaaatat aatcgtgtcc tgcaggatcg cgataggtgt aggagcgttg cggaaatcct
    11101 gccgcatcca gttgcagtac gccaatcatt tcctggttga aaaagttgat aaagtattgt
    11161 gcgttgaagc caaaaacatc aaagtttttg taacctagcg tgtttttaat attgtacaaa
    11221 ttgggctctg tattctggtc aatttgttga atccagctcg ggtaggtcat ttcaattacc
    11281 agcggtatgt ttgtgtttac cgaataccgg tgaagtgaat aatcggaagt gctgtagggc
    11341 tgcagtattc ccgtgatctg aatttcatcc ggagcacgga gcgctaactg tcggctcatt
    11401 tgtaaactga aatttgcagt tagatctgca accaaagtat cagggttgcc aaaattggaa
    11461 tccagcgcaa ttacgtagcc attttggcct ccgcaggcgg agtgataaac gagagtattg
    11521 gtatcggtat acgcgatagc taaaattcta cacgtgttgg caggatcagc aatgatgtag
    11581 gtattggtta gcgatccacc tgagaatttc ttaaacacac tttcatagaa aaaagtgtca
    11641 ggataaaagc ggtcgtatgc ggcaatgaca tcaccattgg gtagtaagtg caatgggaaa
    11701 agtttttgaa ccgaatcaga caaccatgca tgatggtatt gaccggtgct ccattcgaat
    11761 gaggtaagac ggtaagaatc gcctgaatat ccataataga ttagcgtgtc gccgtttggg
    11821 atcgcctcag tatggtacgt aaagggctca ctaagccaaa tctgtgcatg ggttgcgctt
    11881 gaaaaaatca ccgcagttcc acaaaaaaaa cagacaagca aatttcgaag caacataatc
    11941 atcgtttcct gtaaaattcg gaaatctggc ttaaacggcg accgttgttt gatacaaaat
    12001 tccgtattta ttacatctct aaccctaaaa atcgattcta taataaaaca acggcaggaa
    12061 tccgagctgg taattgaaac ttgaataacg tggc
//
LOCUS       DAOMYG010000027        12089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1754_length_12089_cov_11.400698, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000027 DAOMYG010000000
VERSION     DAOMYG010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 12089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12089
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1754_length_12089_cov_11.400698"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(628..2568)
                     /locus_tag="PK610_01690"
     CDS             complement(628..2568)
                     /locus_tag="PK610_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ34663.1"
                     /translation="MKTIRIILLTLLSTVTLFASAQRKYLQEGDDFYKRRMYLEAIAS
                     YKLALQEDVVVKKFYMTQQIAKTYKRLFDYKNAAEWYGKLMEFKDDNTAENMFEYAEI
                     LMNLEQYDKAAEIFKAYCIKSGKPDLAGKYAALCKWPKDNAGMSKSFNTYRTNIETGG
                     RSMGVAIYNNGIIYALPQNQDYAVKTTYYDLAFASAADSINFGTSVVLPGETNRSFYE
                     GTPFLSADGKFLYFTSNATETEKFVEGKKKKAGISSSGMNVLHIYRAEKVNDSWKHIT
                     ELNVNSEEYNCAFPSLSADGKTLYFSSDMPGGFGGFDLYMSTLTNDGKWSAPKNLGTT
                     VNTDRDEFYPYVYGDKFYFSSRGLPGFGGADIYEADLKEGMPGNARNMGKPFNSPKDD
                     FSFIMSTTGKAGYFSSNRDGETGYDHIYYFKKKFVPDTLRGLVKDKITDKPVEGVKVE
                     LLLIAEDGSESVVNSLMTPKNGKWEFLIDPEKNYRVRYTLNGYETEERTVSSINKVGE
                     LDRNEEKKKLSPVYMKPIVKKDNVVRIDNIYFDFDKASIQPESYPILDNIVKFLQENP
                     EARIELSAHTDAVGNDAYNLGLSKKRAQSCFDYLVSKGISPSRLVPVGYGETRLLNKC
                     KKKDDCPEEQHAINRRVEVKFL"
     gene            complement(2592..3533)
                     /locus_tag="PK610_01695"
     CDS             complement(2592..3533)
                     /locus_tag="PK610_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRJ34664.1"
                     /translation="MKTLRYILIAAITMMSTSNNSKAQNQFLVSQYMLHHGFINPAAI
                     ANTNRMSGAAFYRTTWTGFDGAPVLQGINFNTPFSGGNNAAGITILHDKIGVNRNIEI
                     TGTYAYRMKVGMNQYLSFGAAATVSLLQSDFSSVNTTQPNDPIFSASTPTFAMPNFKF
                     GAYYFQPNFYVGLSVPNLLENKIVYATEYQGKTSFNFSAIHFYLNAGYKWAVNRDFDM
                     NFSTMVKNVSGAPLQMDLNAMGLFNKKFGIGLSYRTSKELVGLLNFQLNQSWRLGYGY
                     DFNFNRIGNYSNGSHEIMVVFNMAQVKSHAILESPRF"
     gene            complement(3563..8101)
                     /locus_tag="PK610_01700"
     CDS             complement(3563..8101)
                     /locus_tag="PK610_01700"
                     /inference="COORDINATES: protein motif:HMM:NF040284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ34665.1"
                     /translation="MQNRISCQSWSIPTIAPLRASALLGMMLFLLVSIGTHAQTTTTF
                     KIKYDINLLDLPGGVAQLPGGDYVIGAINSTGIFLQGNLTRVDQSGNVVWCRTMTGSS
                     LATIVNDVIIDNSGNIVVAGDNSPGAFVTKVDAAGNNIWATRISLPNGGGQSSSESAN
                     KIIQANDGNYVIGGSVTYFWDNVGSVRRDTTLMFVSKVNSTTGAVMWSNVIYVPTANP
                     DESFISGVTEVSDGFVFAGDLSEGTGTLNSDGDYPSDAIIFKVNKTTGALIWTRKLGS
                     AGQSEYFSDVTTTSGDKILACGGANGGAIMMRFDLAGNIEWSHRWTNGLTYEMTEVVE
                     TFDGQYAAFGMNTVFAFPFPSFYGTIHKVTTAATPATTFGKRYTTPGALGSISLIPEG
                     IKTQDNGFLMSLMTFGLPDYSIYSVKTDPTGVIAPSGGCAETNFSPSRANYNPTTSLI
                     TPTEYLNYATGGSITITMASVTPVTTIDCRTVICSAPAAPTVSTNPNPATICAGQSVT
                     LTASGGSGVTYRWYTVSSGGTAFATGASTVVSPATTTTYYVEAEDNANPGCVSTRTAV
                     TVTVNLLPNVVATPSPTSVCSGNGPNIALSSNIAGTVGVTSFTWTVSQTGASGASAGS
                     GTTINQTLTATGTSPGTVTYTVTPTANGCTGNTATVTVTVNPTPTASATPNPTTICSG
                     NAPSVALSSNVTGTSFNWTVAQSAGITGGSAASGSSIAQTLNNSGTTAGTATYTVTPS
                     VGGCNGNTITVTVNVNPAPVASATPNPQTICSGQTSSVALSSNVSNTTYAWTVTQTAV
                     SGGANGSGANIAQTLTNGGTTAGNASYVVTPTAGGCNGSPITVTVNVNPIPVATATPN
                     PQTICSGQATSINITSGVAGTTFSWTASQSGVTGASNGTGSSIAQTLSTSGASAGTAT
                     YVVTPSANSCNGTPTTVTVNVNVTPVLGTTSAQNTTICQGQSTQITATGTGATSFSVY
                     DAPSGGTLLGATPLTVNPSTTTTYYVEANNVNGCADPAGRVAVTVTVIPVANPAWTSP
                     GATCVQAGPINLNNLVTGTAGGTWTGTGVTGNIFDPTGLAGQTVNITYTVGTNPCVQV
                     LTQSISVQAVITAAWTSPGTICESDPIINLNSLITGTAGGTWSGSGVTGNTFNPAGLS
                     GTVNITYNVGVAPCQDQVTQAIIINASPLDPTVSASAGSICQGGSTSINASGSGAGVT
                     YNVYDASGNLLGTAPLNVSPSSTTTYFVTATNANNCVNLGGQQQVTVTVNNNPVASAG
                     NNVTICPNTSTTLTATGGGTYEWSTTETSSSISVSPTTTTTYNVEVTNASGCTATASV
                     TVTVYDAGSLTANNDSAYVENTSSVSINTSPNDIGNPGAISIINGPDNGIFTVNGSTI
                     TYDPNDGYIGMDTITYVICDAFCSSYCDTAQVYIRVTREVVIIVPGGFSPNGDGINDN
                     FVIVGLEKYPENELYIYNRWGELVYQATPYNNDWDGLSNGNRTIYGDEVIDGTYFYIL
                     KLDPAIEPLRGSLELRRK"
     gene            complement(8254..8916)
                     /locus_tag="PK610_01705"
     CDS             complement(8254..8916)
                     /locus_tag="PK610_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ34666.1"
                     /translation="MKLLIADDHPIFRKGLKDILQQSFSTVEIIECEAGDKTLEIIRL
                     EKPDVAVLDIDMPHINGLDICQTVYSEGLPTRIIILTMYKEEEMFNKAFLTGAMGYVV
                     KDNSVVEIVECIKEVMLGKKFIGPALQGFLTKFKEADKKKDRIRELLHSLTQAELKTL
                     KLVSQNRASKEIAELLFVSVKTVENYRSRICKKLELDPRNNSLLLWVSENRELLATIK
                     EF"
     gene            complement(8913..10925)
                     /locus_tag="PK610_01710"
     CDS             complement(8913..10925)
                     /locus_tag="PK610_01710"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ34667.1"
                     /translation="MVASIRIPFSALLFLFVLNGTYVRAQSADEILFETLRKKVKLDS
                     YYDSASVFRSGEEAIKLARKLNDRSKEAEILVFYGNHFYYSHNFPKAKEYFTKANLLA
                     LNAGAKSIERLSRIRLTYLFQDYENESEEAEIGFKSILKEAQQNNDPVNIIECYNALA
                     MSAEKRNDTQEAFKNYMEGLRVAEKNQLSYYTAVMLNNIGLIKLNNGEIDEALEDFNR
                     GLELAREEKNPRLAGHLQNNIGLVYIRLNKNDEALAHFRIGIDYAKTINHPKEIAVAY
                     INFSSVLNKIKRSLESLWYLDSAINVMEKNNMKFELSKGYLGKAQVLTDMERYDQAKV
                     VAQTARDVASANNNLEDNAFAHLTLHRIYEKMKLHELALKEYKTFRTLEDSLQNSRNT
                     RAMKELRVQYEVEKKDILLEQERNKATILEQENKLRAFRVRLIVGGGVLIVVVVFIVF
                     YLRNQRNLRKEQQKFSQQLISDLEKERARIARDLHDDIGQSLSAVKSKLTLLAREGER
                     NAHEMEKEIGKVIEQTREISRSLYPSYLEKIGLTRSVARLMEIVQKSTSIECSFDICE
                     EIEQLSLDKKTHLYRILQECVNNTIKHSGAAALKISIEVKDDDFELTYRDNGKGIQDN
                     DETKGMGFLSIKERARMLGGEMEIGDNSRKGFRLIITFNMERNRKE"
     gene            complement(10952..>12089)
                     /locus_tag="PK610_01715"
     CDS             complement(10952..>12089)
                     /locus_tag="PK610_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009414192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HRJ34668.1"
                     /translation="GKSDKDLRRAYMLFRLIGRPWMVRLGKTMTNLALAMRLPIRGIV
                     KRTIFRHFCGGETIEECESRVIELGKFGIGTILDYSVEGKRAEEDLDATSREIIRTAK
                     VASGNQHIPFCVFKVTGVARFGLLEKLSASKELSEEEKQEYLRVVNRVDAICREAYTA
                     ETPVFIDAEESWIQPAIDGIADAMMAKYNRQKSIVFNTLQMYRHDRLDFFRHSIEKAR
                     KGGYRIGMKIVRGAYMEKERNRAAEKNYPSPIQPDKSASDRDYDEALRLAVDNLDVTA
                     ICAGSHNENSALLLTRLMREKGIPANDTRIYFAQLLGMSDHISFNLSNAGYNVAKYVP
                     YGPVREVLPYLIRRAEENTSVKGQTGRELSLIIKERQRRKKLQR"
BASE COUNT         3129 a         2567 c         2569 g         3824 t
ORIGIN      
        1 gcggaggaag cgtgagaggc agccggcagc agcggcgcgc gcgacatccg ggccgaccat
       61 ttttgatttt gtgcaaaggt gtgtgcgaaa cacaaagcac ctagcaacgt tgcagcgagt
      121 cgaattcttt tcatagtaga tagttttcct taggataaat cctatgtgtt aaaaatagct
      181 caggaacgaa tttcctttca gtagggctgc cccctcaatt gatggaggaa gaagaatgaa
      241 aaacaaaaac tgaaacaatt gtgaaaaaat cccgccggta taaaattgaa aacctacttg
      301 cctttatttt cagaagagat accgacggga attaactcct tatggaagaa ttaccggatc
      361 aatgatgatg cgtttcatac aaaccaaatt aaatgatggt tcaaatgtat agggcaactt
      421 atatcgattt agtgggggca gcccctcaat acctttcttt ttgattttca atgctttgat
      481 gggtgatgag cgctggattt gtgaagattt attacaaagc ttatttcgct ttgtgaaaaa
      541 aagaggttgt cgtcgggagg aaaactactg tcgtgaaaag aacgggaccg acatagtgtg
      601 gaaaggaccc gacgacaacc tggttcttca gaggaatttt acttctactc ttctattgat
      661 ggcatgctgt tcttccggac agtcatcctt tttcttgcat ttattaagta gtcgtgtttc
      721 accataacca acaggaacaa gtcgcgaggg tgaaatgcct ttactaacga gataatcaaa
      781 acaagactga gcgcgctttt tggagagacc aagattatag gcatcatttc caactgcatc
      841 agtgtgtgca cttaattcaa tgcgtgcttc cggattttcc tggagaaatt ttacaatgtt
      901 gtcgaggata ggatacgatt ccggttgaat acttgccttg tcgaaatcaa aatagatatt
      961 atcgattcgt acaacattgt cctttttaac aattggcttc atgtaaacag gactcaactt
     1021 tttcttttct tcattgcggt cgagttcgcc aactttattg atggatgaaa cggtgcgttc
     1081 ttcagtttca tatccattca atgtgtatcg tacgcggtag tttttttccg gatcgatcag
     1141 gaattcccat ttgccgtttt taggagtcat tagagaattc acaacagatt cgctgccatc
     1201 ttctgcaatc agtaacagtt ccactttaac tccttcaaca ggtttgtctg tgatcttgtc
     1261 ttttacaagt ccgcgaagtg tatcgggaac aaatttcttt ttgaaatagt aaatgtggtc
     1321 atagcctgtt tctccatccc ggtttgaact gaaataccct gcttttcccg tagtactcat
     1381 gataaatgag aaatcatctt ttggagagtt aaatggttta cccatgttgc gtgcattgcc
     1441 gggcattcct tccttcagat ctgcttcgta aatatctgca ccaccaaaac cggggagtcc
     1501 tctcgaagag aaataaaatt tatcgccgta aacataaggg tagaattcat cgcgatcggt
     1561 gtttacagtt gtgccgagat ttttcggtgc cgaccatttt ccgtcgttgg ttaatgttga
     1621 catatacaaa tcaaagcctc cgaaacctcc gggcatgtct gatgaaaaat aaagtgtttt
     1681 tccgtcggca cttagtgaag gaaaggcaca attgtattct tcagaattta cgttaagctc
     1741 tgtgatgtgt ttccaggaat cgtttacttt ttctgcgcgg tagatatgca gaacattcat
     1801 tccggaagag ctgattcctg cttttttctt tttgccttca acaaattttt cagtttccgt
     1861 tgcattggaa gtgaaataga gaaattttcc atctgcactc agaaagggag tcccttcata
     1921 aaaggagcga ttagtttcac ccggcaaaac aacagaggtt ccaaaattta tggaatctgc
     1981 tgccgatgca aaagcaagat cgtaataggt tgtttttact gcgtaatcct gattttgagg
     2041 taatgcataa ataatgccgt tgttgtatat cgctacaccc atacttcttc caccagtttc
     2101 aatattggtg cgataagtgt taaaggattt gctcatgcca gcattatctt ttggccattt
     2161 gcaaagggct gcatatttgc ctgctagatc cggtttgccg gatttgatgc aatacgcttt
     2221 gaaaatttca gctgccttat catattgctc aaggttcatc agaatctctg catactcaaa
     2281 catgttttct gccgtattgt cgtctttaaa ctccatgagt ttgccgtacc attctgctgc
     2341 atttttatag tcaaacaaac gtttataggt ttttgcaatc tgctgcgtca tgtagaattt
     2401 cttcactaca acatcttctt gcaaagcgag tttgtaactg gcaattgctt ccaggtacat
     2461 tcttctctta taaaagtcat caccttcctg gaggtatttg cgctgtgctg atgcaaatag
     2521 agtgacagtg ctcaacagtg tgagcaaaat aattcgaatt gttttcatac ttttttcctt
     2581 gcaggtgtac tttagaagcg gggtgattca agaatggcgt gcgattttac ctgagccata
     2641 ttaaatacaa ccatgatttc gtgactaccg ttgctgtaat tgccaattcg gttgaaattg
     2701 aaatcatacc cgtatccaag tctccaggac tgattcaact ggaagtttaa caatcctacg
     2761 agttctttgc ttgttcgata agaaagtccg atcccgaatt ttttattgaa caatcccatt
     2821 gcatttaggt ccatttgaag tggtgcgcct gaaacattct taaccatggt agaaaaattc
     2881 atatcaaaat cacggttaac agcccacttg tatcctgcat tcaggtaaaa gtgaattgcg
     2941 ctgaaattga aactggtttt accctggtat tctgttgcgt aaacaatttt attctccaac
     3001 agatttggaa cagacagacc aacataaaaa ttaggttgga aataatatgc accgaacttg
     3061 aaatttggca ttgcaaaagt aggagtgcta gccgaaaaaa tcggatcgtt tggttgtgtt
     3121 gtattaacag aactaaaatc actttgcaat aatgaaactg tggctgctgc tccgaacgaa
     3181 agatattggt tcattcccac tttcatgcgg taggcatagg ttccggtaat ttcaatgttg
     3241 cggtttactc caatcttatc atgaagtata gtaatgccag ctgcattatt gcctccacta
     3301 aatggggtat tgaaattgat tccctgcaat accggagcgc catcaaaacc agtccaggtg
     3361 gtgcggtaaa atgcagctcc actcatgcgg tttgtattgg ctattgcagc gggattgatg
     3421 aatccatgat gcagcatgta ttgcgatacc agaaactgat tctgtgcttt ggaattatta
     3481 ctcgtgctca tcatagtgat ggcagcgatc aaaatatagc gtaaggtttt catgttcttt
     3541 tctattaaag ttctaccaac aattattttc ttctcaattc aagtgaacct cttaatggtt
     3601 cgatagctgg atcgagtttg aggatataaa agtaggttcc gtctataact tcatcaccat
     3661 agatagtgcg attgccattc gataaaccgt cccaatcatt gttgtaagga gttgcctgat
     3721 aaaccagttc tccccaacga ttgtagatat acaattcgtt ctccggatat ttttccaaac
     3781 caacaattac aaagttgtcg ttaattccat caccgttcgg actaaatcct ccagggacaa
     3841 taattacaac ttctcttgtt acccggatat aaacctgtgc agtatcgcaa tagctggagc
     3901 aaaatgcatc acagatcaca tacgtaatcg tgtccatacc aatgtacccg tcattcggat
     3961 cgtaggtaat tgtgcttcca ttcacagtga agattccatt gtcaggacca tttataattg
     4021 aaatggcacc cggatttcca atgtcattgg gagaagtgtt gatcgaaacg cttgaagtat
     4081 tttctacata agcagaatca ttgtttgctg taagcgatcc tgcatcgtat acagttaccg
     4141 ttacacttgc cgttgctgta catcctgatg cgttggttac ttctacattg taggtagtgg
     4201 tggtagttgg acttacactg atgctggacg atgtttctgt tgtgctccac tcatacgttc
     4261 ctccacctgt tgcagtaagg gtagttgagg tgttcggaca aatcgttaca ttatttccgg
     4321 cacttgcaac aggattgtta tttacagtta cagttacctg ctgttgtccg ccaagattta
     4381 cacaattgtt tgcgtttgtt gcagtaacaa aatatgtagt tgttgatgat ggagaaacat
     4441 tcaaaggcgc tgtaccgagt aaatttccgg aagcatcgta aacattgtaa gttacacctg
     4501 caccggagcc tgatgcgttg atgctggttg atcctccctg acaaatactt ccagcacttg
     4561 cgcttactgt tgggtcaagt ggtgatgcat taataataat tgcttgggta acctgatcct
     4621 gacaaggtgc tacaccaaca ttgtacgtga tgttcactgt tcctgaaagt ccggcaggat
     4681 taaatgtatt tccggttaca cctgatccac tccatgttcc acctgctgtg cctgtgatga
     4741 gagaattcag attgataata ggatcagatt cgcaaatggt gccgggtgat gtccatgctg
     4801 cggtaattac tgcctgcaca cttatgcttt gagtgagtac ctgtacacaa ggattggtac
     4861 ctacggtata ggtaatgttt actgtctgtc ctgccaagcc ggttggatca aaaatgtttc
     4921 ctgttactcc tgttcctgtc cacgttccgc ctgcagttcc tgttacaaga ttgttcaggt
     4981 taattggacc agcctgcaca caagtagcac ctggtgaagt ccatgccggg ttggcaacag
     5041 gaatcactgt aactgtaaca gcaactcttc ctgccggatc agcacaaccg tttacattgt
     5101 tggcttcgac ataataagta gtagttgttg atggattcac tgtaagtggt gttgccccca
     5161 ataatgttcc acctgatgga gcatcgtaaa cactgaatga tgttgctcct gttcctgttg
     5221 cagtgatctg cgttgattgt ccctgacaaa ttgttgtatt ctgtgcactt gtagttccga
     5281 gaacaggggt gacgtttacg tttacggtaa ccgttgttgg agttccgtta cacgagtttg
     5341 cactcggagt tacaacgtat gttgcagttc ctgcgcttgc cccgctggtg ctgagtgttt
     5401 gtgcaatgct gcttcccgtt ccgttgcttg caccggtaac accgctttgt gatgctgtcc
     5461 agctaaacgt agttcctgca acacccgaag tgatgttgat ggaagttgct tgtcctgaac
     5521 aaatggtttg cggatttggt gtagctgttg caaccggaat cggattaacg ttcacagtaa
     5581 ctgtaattgg agaaccatta catccacctg cagttggtgt tacaacataa ctggcgtttc
     5641 ctgcagtggt tccgccattg gtaagtgttt gtgctatgtt tgcaccgctt ccgttcgcac
     5701 cgccgcttac tgcagtttgg gttactgtcc atgcgtaagt ggtgttgcta acattcgaag
     5761 aaagtgcaac gctgctggtt tgtccggaac aaatggtttg cgggtttggt gtagcacttg
     5821 caacaggcgc aggatttacg tttacagtaa cagtgatcgt gtttccgtta catcctccaa
     5881 ctgaaggtgt aactgtgtaa gttgcagttc ccgctgttgt tccgctgttg ttgagcgttt
     5941 gtgcaatgct tgaacctgat gctgcactac ctccggtaat tcctgcagat tgtgctacgg
     6001 tccagttaaa tgatgttcct gtaacattcg aactcaatgc aacacttggg gcatttccgg
     6061 agcagatcgt tgttgggtta ggtgtggcac ttgcagttgg agtgggattc acagttacag
     6121 ttactgttgc tgtgtttcct gtacaaccat ttgcagtggg tgttacggta taggttactg
     6181 ttccgggcga tgttcctgtt gcagttaatg tttggttgat tgttgtacca gatcctgcac
     6241 ttgcaccgct tgcgccggtt tgagatacgg tccaggtaaa gctggttaca ccaactgttc
     6301 ctgcaatgtt ggaacttaag gcgatgtttg gaccgttacc tgaacatacc gatgttggag
     6361 aaggtgttgc cacaacatta ggaagtagat taactgttac agtaactgca gtacgagtac
     6421 tcacacaacc ggggttggcg ttgtcttctg cttcaacata atacgttgtt gttgttgcag
     6481 gagaaactac tgttgatgcg cctgtggcaa atgctgtacc tccggaagat acagtatacc
     6541 agcgataggt tacaccactt cctccgcttg cagtaagcgt tactgactga cctgcacaaa
     6601 tggtggcagg attaggattg gtagaaaccg ttggtgctgc tggtgcagaa caaataacag
     6661 tgcggcaatc gatagttgta accggagtta cacttgccat tgtgattgta atggaaccac
     6721 ctgttgcgta attgaggtat tctgttgggg taattaatga agttgttgga ttgtagttcg
     6781 cacgcgaagg tgagaagttg gtctctgcgc atcccccgct cggggcaata acaccggttg
     6841 ggtctgtttt tacggagtag atggagtaat ccggaagccc aaatgtcatt aacgacatta
     6901 aaaatccgtt atcctgtgtt ttgattcctt caggaattag agaaatgctg cctaaagctc
     6961 ccggagtggt gtatcttttt ccaaaagttg ttgccggagt tgctgctgta gttaccttat
     7021 gtattgttcc gtagaatgaa gggaaaggga acgcaaatac agtattcatt ccaaaagctg
     7081 catattgccc atcgaatgtt tcaaccactt ctgtcatttc ataggttaaa ccgttagtcc
     7141 atctgtgact ccactcaata tttccggcaa ggtcaaaacg catcataatg gctccgccat
     7201 ttgcaccacc acaggccaga attttatctc ctgaagttgt agtaacatca ctgaaatatt
     7261 cggactgtcc cgctgaaccg agtttcctcg tccatattag ggctcctgtt gttttattca
     7321 ctttaaaaat gatggcatct gatggataat cgccatcact gtttagcgtt cctgttcctt
     7381 ctgaaagatc gcctgcaaaa acgaatccgt cggaaacttc tgttactcct gaaataaaac
     7441 tttcatccgg atttgccgtt ggaacatata tcacattgct ccacattaca gcacccgttg
     7501 tggagttgac cttactaaca aacattaacg tggtgtctct tcttacactt ccaacgttat
     7561 cccagaagta agtcacactt ccgccaatta cataatttcc gtcattggcc tggatgattt
     7621 tgtttgcaga ttctgatgag ctctgacctc ctccgttggg aagggaaatt cgtgtagccc
     7681 aaatgttatt tcctgccgca tcaacctttg ttacaaatgc accgggtgaa ttatcacctg
     7741 ctacaacaat gttgccgctg ttatcaataa taacgtcatt aacgatggtt gctaaagatg
     7801 atccggtcat cgttctgcac catactacat ttcctgattg atctacacgg gtcaaattac
     7861 cctgcagaaa aatgccggtg ctgttaatag ctccgatgac ataatcacca ccgggaagct
     7921 gtgcaacacc accgggaagg tccaacaaat taatgtcgta cttgatctta aaggttgttg
     7981 tagtttgtgc atgtgttcca atactaacaa gaaggaacag catcatcccc agcagcgcac
     8041 tggcacgaag tggggcaatg gtcgggatcg accaagattg gcaagagatt cggttttgca
     8101 taaataagta gttttccgat gccaaaagta ttcccggtaa ttggtatgga ttagaggggc
     8161 gccccctcaa aaaaaccggc taaaccgttt aattttaccg attaacaata ctaggccttc
     8221 gatgaataaa atttaacaag ccctggttcg ggtctaaaat tccttaatcg tagcaaggag
     8281 ttctctgttt tcactcaccc ataataacag gctgttgttc cgagggtcaa gttccagttt
     8341 cttgcagatc ctggatcggt agttctcgac ggtctttaca gaaacgaaga gcagttcggc
     8401 tatttctttg ctcgcccggt tttgggatac cagttttagt gtcttcaatt cggcctgagt
     8461 cagcgagtgc agtaattcac gaatacggtc tttcttttta tcggcttctt tgaactttgt
     8521 taggaatccc tgaagggcgg gaccaataaa tttcttgccc agcattacct ctttgatgca
     8581 ttccacaatc tcaacaacag aattgtcctt tacaacataa cccattgctc cggtaagaaa
     8641 tgctttgttg aacatttctt cttccttata catggtaaga ataataatgc gggtaggtaa
     8701 tccttcgcta taaacagtct gacaaatatc gagaccgtta atgtgcggca tatcaatgtc
     8761 gagtacggca acatcgggtt tttcgagccg aataatttcg agcgttttat ctccggcttc
     8821 gcattcgatg atctcaaccg tcgaaaaaga ctgctgaaga atgtctttca aaccctttct
     8881 gaaaatagga tggtcatcag caatcagtag tttcattctt ttcgattcct ttccatgtta
     8941 aaggtaatga ttaatcgaaa cccttttctg gaattgtctc cgatttccat ttctcctccg
     9001 agcattcttg ctctttcctt tatactcaga aagcccattc cctttgtttc atcattgtct
     9061 tgtataccct tgccattatc acgataggta agttcaaagt catcgtcctt tacttcaatg
     9121 gaaattttta gtgctgctgc accactgtgt ttgatggtat tattcacaca ttcctgcagt
     9181 atcctgtaga gatgtgtttt tttgtcgagc gacaattgtt caatctcttc acaaatatca
     9241 aaactgcatt caatagaggt gcttttctga acgatctcca tcagtcgtgc tacacttcga
     9301 gtaagtccta ttttttcaag ataggagggg taaagtgaac gggaaatttc acgcgtttgt
     9361 tcaattactt ttccaatttc tttttccatt tcatgcgcat tccgctctcc ttcacgtgcc
     9421 agtaatgtga gctttgattt tacggcggat aaactctgcc cgatatcatc atgcaaatca
     9481 cgggcaatcc gcgctcgttc tttttcgaga tcggaaataa gttgttgact gaatttttgt
     9541 tgctccttac ggaggttgcg ttgattgcgt aaatagaata ctatgaaaac aacaacaaca
     9601 atcagaactc ctcctccaac aatcaatcgt actcggaaag cgcgcaattt gttttcctgt
     9661 tcaagaatgg tggctttgtt tctttcctgc tcgagaagaa tgtctttctt ttcaacttca
     9721 tattgtaccc gcaactcttt catggctcgg gtgtttctgg aattttgaag gctgtcttca
     9781 agtgtgcgaa atgttttata ttcctttaga gcaagctcat ggagcttcat tttttcgtag
     9841 atgcggtgta aggttaaatg agcgaatgca ttgtcttcaa gattattgtt tgcactggct
     9901 acatctcttg ctgtttgcgc cacgacttta gcctgatcat agcgttccat atcggttaat
     9961 acctgggcct ttccaagata acctttactc agttcaaact tcatattgtt tttttccatc
    10021 acattaatgg cagagtcaag ataccagagt gattccagtg aacgttttat tttattaagc
    10081 actgaactga aattgatata agcaaccgcg atttcttttg ggtgattgat ggtttttgca
    10141 taatcaatac caatgcgaaa atgagcaagt gcttcatcgt ttttgttaag acgaatatat
    10201 accaatccga tattgttttg caaatgtcct gcaagtctgg ggtttttttc ctcccgtgca
    10261 agttccaatc ctctgttaaa atcttccaac gcttcatcga tctcaccatt gttcagtttg
    10321 atgaggccaa tgttgtttaa catgacggcc gtataatagg agagttgatt tttttcagca
    10381 actcttaatc cttccatata gtttttaaaa gcttcctggg tgtcgttcct cttctctgcc
    10441 gacattgcaa gtgcattgta acattcaatg atgtttactg gatcattgtt ttgctgcgct
    10501 tcttttaaaa ttgacttaaa tccgatttca gcttcttctg attcgttttc gtaatcctga
    10561 aaaagatagg tcagacgaat tctcgagaga cgttcaattg attttgcgcc ggcatttaaa
    10621 gcgagcaaat tggctttggt aaaatattct ttcgcttttg ggaaattgtg gctgtaataa
    10681 aagtggttac cgtaaaaaac caggatttct gcttctttgc ttcggtcatt taattttctt
    10741 gccagtttaa ttgcttcttc acccgaacga aaaacagagg ccgaatcgta atacgagtcc
    10801 agttttactt ttttccttaa tgtttcaaac aggatttcgt cggcagattg tgctctgaca
    10861 taagttccgt tcagtacaaa cagaaacaat aaagcactaa aaggaattcg gattgatgca
    10921 accataattg caccaagata gtgaatgcga atcagcgctg caatttttta cgacgctgtc
    10981 tttctttgat aatcagactt aattctctgc ctgtttgacc ttttacactc gtgttttcct
    11041 cagcgcggcg aataagatag ggcagaacct cccgaacagg tccgtaagga acatattttg
    11101 caacattgta tcctgcatta ctcaggttaa agctgatgtg gtcgctcatg ccaagtaatt
    11161 gtgcaaaata aatacgggta tcgtttgcag gaattccttt ttcccgcata aggcgggtga
    11221 gaagtaaagc gctattttca ttatgactac ctgcacagat tgcggttaca tcaagattgt
    11281 caacagcgag acgcaaagcc tcatcataat cccggtcgga tgctgatttg tcgggctgaa
    11341 taggggaggg gtaatttttt tctgcagcac gattacgttc tttttccata tatgcaccgc
    11401 gaacgatttt cattccgatc ctgtatccgc cttttcgcgc tttctcaatg gaatggcgaa
    11461 aaaagtcgag ccggtcgtga cggtacattt gcaatgtatt gaacacaatg gatttttgtc
    11521 tgttgtattt agccatcatc gcatcggcaa ttccatcaat ggccggttga atccagcttt
    11581 cttcagcatc aataaaaacc ggtgtttctg ctgtgtatgc ttcacggcaa atagcatcca
    11641 cacggtttac cacgcgcagg tattcttgtt tttcttcttc agaaagttcc tttgatgcac
    11701 ttaatttttc gagtaatcca aagcgtgcaa ctccggtgac tttaaaaaca caaaagggaa
    11761 tatgttgatt tccactggca acctttgctg ttcgaatgat ttcccgggaa gtcgcatcaa
    11821 ggtcttcttc agcacgtttt ccttcaaccg agtagtcaag aatggttcca attccaaatt
    11881 tccccaattc gatgactctg gattcacatt cttcaatggt ttcaccgccg cagaagtgac
    11941 gaaaaatggt tcgtttaacg atacctctaa tgggaagtcg catcgcaagt gcgagattgg
    12001 tcatcgtttt tcccaacctt accatccaag gcctccctat taatcggaaa agcatgtatg
    12061 ctctgcgaag atccttatcg ctctttccg
//
LOCUS       DAOMYG010000028        11705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1829_length_11705_cov_9.711416, whole genome shotgun sequence.
ACCESSION   DAOMYG010000028 DAOMYG010000000
VERSION     DAOMYG010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 11705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11705
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1829_length_11705_cov_9.711416"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="PK610_01720"
     CDS             complement(<1..1431)
                     /locus_tag="PK610_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HRJ34669.1"
                     /translation="MKTIIGIVVCLFLTRGTVSAGGGNALALNFDGSNDYVQTQVPYS
                     LSSWTYECWVIAPTAPGSLNSSGPMYGENAGITWNHSSSAFRGVAFVRDASGAYLSAS
                     FGALSSNTWYHLAATYDGSVLKSYKNGVLMNTTTSVGGGVFSPVSGNLKLGAHPLLSG
                     FFSGIIDEARVWDYARTCEEINDHMNLEFYSSSTGLIASYNFNESASVNGNNTSITAL
                     YNSANSVADGVLTNFGLNGAGSNFITGGPFNAPLQCQGGGTSVACEALRFDGVDDHIV
                     LPTDGVFNVTEGTIEAWIKTSASNSGYQGIVVKEFAYGIFLMNNKLGAFNWPIGTAIT
                     TTTNLNDGNWHHVAMAFEIGIPSGIKLYIDGSLSATGTIINVSGQSGTLIIGNNESQN
                     SYFEGSIDGVRFWNRALCGEEIFNNMNCQIVGAVPDLVAVYNFNQGQTFTDNSSEITL
                     VDASGNGYDGMLTNFNLSSGNTTSNWV"
     gene            1659..2585
                     /locus_tag="PK610_01725"
     CDS             1659..2585
                     /locus_tag="PK610_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34670.1"
                     /translation="MKRDMNKNILLGGIAVIAAGVFFACGGGEADSSLNKVDSTKIAG
                     DTIDDTKSKIEQAQKVYYSIPSTSEMASLLTTAGAEYNMKLVNDINFVDKYTTPRKQA
                     LNLGIYGADLNYCSIYERTNETMFYMECTRSLAEKLGIENAITDETVTRAEENIDNRD
                     SMLTIISDMFYELEEYLAENDKDELSALVIGAGWVEGLYLATQTVNEKKPNQDIMSRI
                     AEQKLSLGNLMKFIEQYIADEDVKSLFDDLTRISKIYEGIVVEKQETKVSTDSDGKTV
                     IGGTTKVEMSTEQFKELRKVVKEIRDSYTKIK"
     gene            2588..3028
                     /locus_tag="PK610_01730"
     CDS             2588..3028
                     /locus_tag="PK610_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34671.1"
                     /translation="MKFRHILFLALVAFLGMAQEADAQCKSYVKRNCRPGLTPYLHNG
                     QVNNAILVPGDKAELLLTFFAGQEYRLMICSMPVLGAVSFRVLDSDRNPIYESKKNPS
                     QNYFDFKVASTQQLIVEINVPEAKPNNDIVPEGCVSVLIGFIKK"
     gene            complement(3276..5378)
                     /locus_tag="PK610_01735"
     CDS             complement(3276..5378)
                     /locus_tag="PK610_01735"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HRJ34672.1"
                     /translation="MKKTMLLLGAVFASTLLSAQLREQHVEPCGQHRFTEKVKQEVGY
                     NAAHQLAEDAYEAEIQQIIANQTPEMRGGSAVYVIPIVFHIVHEGGTENISDAQVQDA
                     VFILNRDFNKQNADTSAVVSAFANIIANVGIEFRLAQYDELGACVSGITRTYSTKTND
                     GDRPMVDDVNRNLNNSPTNTNNVRFPRNHYLNIWVCKNADGAAGYTMTPNGFTPAKYD
                     GIWVNHSYVGSIGTGAVQRSRTLTHEVGHWLNLSHVWGSTNNPGVSCGDDNVTDTPQT
                     MGWNTCNINGATCGNTIDNVQNYMEYSYCSRMFTNGQKARMIAALNSTTGQRNQLWQA
                     QNIIDAGVTGVDNLCAADFSTFNQVVCVGQSIDFTDNSFHGPSNWSWSFTGASPATST
                     DQNPTGIVYSAPGTYAVSLTVGNGSVTQTATKNSYITVLPYNGLGTPFSEGFESATTV
                     PNANWFINNFDGGQAWQVSSLAATTGTKCIRLNNSAQSAGAVDEIQSVTYDMSMYGSM
                     QLSFKYAFAYKNSSTNTDKIKVYASSDCGETWQLRRNIASTSLPTAPNTTAAFTPNSS
                     QWVQVDVTNLAAYLVPNFMFKISFEAGGGNNVYIDDINLSGAVAVEEADNDFAFSIFP
                     NPSSDLANLVFVLNSNETVSIELFDMLGKVVMGRNLGNLSSGEHNLSVPRGDLASGVY
                     YVRLNVGGQSVVKKLILE"
     gene            complement(5394..6113)
                     /locus_tag="PK610_01740"
     CDS             complement(5394..6113)
                     /locus_tag="PK610_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ34673.1"
                     /translation="MDHYFITGTGTGIGKSLVEELLGKAKITGYSRSNQIHHPDYTHH
                     SIDLSHPGAVANMPLELPDDADRVVLINNAGTIGEINRTGDLSQEHYQQVFQLNLVSL
                     TQICDRFLNQARTKNYPCLLLNISSGAAINPIPSWAAYCASKAGVDMFTRVLNAELQE
                     LKLDHIRVFSVYPGVVDTGMQAYIRSSDPGNFSSLLRFQEYKDEGKLYRPEQVTAAIL
                     SIFKDLSALTEPVVNLRDYIQ"
     gene            complement(6117..6818)
                     /locus_tag="PK610_01745"
     CDS             complement(6117..6818)
                     /locus_tag="PK610_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRJ34674.1"
                     /translation="MPLHGNKFAVIGVGRYGSSIARRLAQKGAEVYCFDQDEEKIDNV
                     KDDVAYAATLDSTDDKALVSQNISDVDGVVVAIGENFEATILTCAHLIDLGIKRVIAR
                     AHGPQQKMILEKIGVKEILLPEEEVAFVVAERLLNPSILSFLQLPDDYEIAEIRAPKN
                     IVNRTLNDIGLRDKYRLTLITIKRSFDGKDGADVVKEDHILGVPKSESVVYDTDTLII
                     FGTSHDVARFIDINQ"
     gene            complement(6855..8009)
                     /locus_tag="PK610_01750"
     CDS             complement(6855..8009)
                     /locus_tag="PK610_01750"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020023248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HRJ34675.1"
                     /translation="MKDTSKLGFGTRAIHAGMEPDPSTGAIMTPIFQTSTFVQESPGK
                     HQGYAYARGKNPTRAALEKNIAALEKAKHGLCFSSGMGAMDAVIKLLQPGDEVITGDD
                     LYGGSYRMFTKVFAPFGIKFHFVAMNNAENIRNYINANTKMIWLETPTNPTMQIIDIA
                     ACAAIAKEKNLLLAVDNTFASPYLQNPLDLGAHIVMHSVTKYLGGHSDVVMGALVMND
                     DKLFERLAFIHNSCGATPGPMDSFLVIRGIKTLHLRMERHCSNGEKIAHFLRSHPKIE
                     KVYWPGFEDHPNHAIAKKQMRGFGGMISFVYKGNIEETFKLASSFNVFSLAESLGGVE
                     SLINHPATMTHASIPKEEREKAGVVDSLLRLSVGVEDCDDLLADLKQALG"
     gene            complement(8095..9246)
                     /locus_tag="PK610_01755"
     CDS             complement(8095..9246)
                     /locus_tag="PK610_01755"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRJ34676.1"
                     /translation="MLPLLLGLAVAFWMVRNAVAEEKFVESERGNYIWTDTNRNGIVD
                     FSNANDFSPVAEGGNFRRISFAESWRSIDWTWYSSLCILLACFMMVLRDAAYMWRIRI
                     LTDKELSWRQSFRVIMVWEFASAMTPGVVGGAAVAMFILNREKINMGRSTALVLITAL
                     LDELFYILIVPVLILIAGSASLFPVEFTGNFLGMEFGVLEVFWIAFGIIAAITVLLFA
                     GIFIVPQGLRSLLLFFCRLPFLRRLKEKAERTGNDIVIASTELRGKSRSFWLQAFLAT
                     VISWTGRFLVINFLIMAFAPMADQFIVFARQLSMWVLLMVSPTPGGSGIAEFAFSGFL
                     AEFVPFGLAGMLAVLWRLISYYPYLLIGAMVIPGWLSHTSDTVAPPRKP"
     gene            9345..9698
                     /locus_tag="PK610_01760"
     CDS             9345..9698
                     /locus_tag="PK610_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013750791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HRJ34677.1"
                     /translation="MKKIYHLSTCSTCKRILSELKPGADVVLQDIKSDALTETQVDDM
                     AKLAGSYEAIFSRVAMKYRALGLDKKTLSEKDYKKYILEEYTFLRRPVVLADNRIFIG
                     NSRKIVESAAEALRK"
     gene            9829..10431
                     /locus_tag="PK610_01765"
     CDS             9829..10431
                     /locus_tag="PK610_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HRJ34678.1"
                     /translation="MIKRQLNRSYRISKYVFYRETLVDSKELFWSFIGSFIGIGCIAF
                     LQSLYLTETENVFLIGSFGATGVLIYGAIQSPLAQPRNLIGGHLISAFIGVTVYKIIP
                     DIIWLSAPLAVSLSIAAMQFTKTLHPPGGATALIAVIGTEKIKALGYLYIVTPVLSGV
                     LIMLIVALVFNNITPMRSYPVKGRFARMVNVFGKNKNKGK"
     gene            10534..11067
                     /locus_tag="PK610_01770"
                     /pseudo
     CDS             10534..11067
                     /locus_tag="PK610_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008638810.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
     gene            11171..>11705
                     /locus_tag="PK610_01775"
     CDS             11171..>11705
                     /locus_tag="PK610_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34679.1"
                     /translation="MSEVNTPRLMAAAKEFNIGTNTLIEFLVSKNFSSDDLKPTSRLT
                     EPMYRVLQAEFQQDKVARQKAEQIDLPKGVSASEQKKKKDEEDLSIRKKEKEKEEKAK
                     AEEKKPEPVEEAPVAAPAAEKEEPVAETVSGNEVTKIEAPELEGPKIVKKIDLDAIDS
                     STRPKKTTKKAAPEEVTP"
BASE COUNT         3477 a         2695 c         2509 g         3024 t
ORIGIN      
        1 tacccaattg gatgtagtgt tgccggaaga aagattaaaa ttggtaagca ttccgtcata
       61 tccattaccg gaggcgtcga ccaaagtaat ttctgaagaa ttgtcggtaa atgtttgacc
      121 ttggttaaaa ttgtaaaccg ccaccaggtc tggcactgct ccaacaatct gacaattcat
      181 gttattgaag atttcctcac cgcacaacgc tctgttccaa aagcgaactc cgtcaataga
      241 tccttcaaaa taactatttt gtgattcgtt gtttccgata attaatgtcc cgctttggcc
      301 ggacacatta ataattgtac cagttgcaga gagcgagcca tcgatatata atttaatgcc
      361 actgggaatg ccaatttcaa aggccatagc aacatgatgc cagttgccgt cattcaaatt
      421 ggttgttgtt gtaatggcag tcccaatcgg ccaattgaag gcgcctaatt tattgttcat
      481 taggaaaatt ccgtatgcga attcttttac aacgatgccc tgataaccgg aatttgaagc
      541 tgatgttttt atccatgctt caatcgttcc ttctgttaca ttgaatacac catcggtagg
      601 aagtacaata tgatcgtcga caccatcaaa acgcaacgct tcgcaagcaa ctgatgtacc
      661 accgccttgg cattgcaaag gagcattaaa tggaccacct gtaataaaat ttgatccagc
      721 cccgttcaaa ccaaaattcg ttaatactcc gtctgcaacc gaattggccg aattgtacaa
      781 agcggtaatg ctggtattgt ttccgtttac agatgcggat tcattgaaat tgtaacttgc
      841 aatcaaacct gtgcttgacg aataaaattc aaggttcatg tgatcattga tttcctcaca
      901 cgtacgcgca taatcccaaa cacgggcctc gtctattatc ccggaaaaaa atccagataa
      961 taaaggatgg gcacctaatt ttaaattacc tgaaaccgga gaaaacacgc cgccaccaac
     1021 agatgtagtg gtattcatta aaacaccgtt tttataggat ttcaatactg atccgtcgta
     1081 agtagcggcc aaatggtacc aggtattaga agataatgcc ccgaaggatg ccgaaaggta
     1141 tgccccagat gcatctctaa caaaagcaac tcctctaaag gctgaactac tgtgattcca
     1201 ggtaattcct gcattttcac catacattgg tccgcttgaa ttcaaacttc cgggtgcagt
     1261 tggggcaatt acccaacatt cataggtcca agacgacaaa ctatagggaa cctgggtttg
     1321 cacatagtca tttgatccat caaaattcaa ggccaaagca ttgccgccgc cggcacttac
     1381 agttcccctg gtaagaaaaa ggcacactac aataccaata attgttttca tgtttaagat
     1441 ttttgccaaa acacgacaaa gttggctact tgatgaaatc tactttgtgg agtggcagta
     1501 tgagtttgtg aatgacctac aaaaaattaa tatcggaata tcagcccaac aaatcgcgta
     1561 aacacaagca actctcaaaa tcctgttgat aaaatcgcct atcaacagca ccggcccctc
     1621 atcgggtgtg tttgaattcc tttatatttg attaccaaat gaaacgcgac atgaacaaga
     1681 acattctgct gggcggtatt gcggtgattg ctgctggagt atttttcgcc tgcggtggcg
     1741 gagaagccga cagctcactc aataaagtgg acagcaccaa aattgccggt gacaccatcg
     1801 acgacaccaa gagcaagatc gaacaggctc agaaggttta ctatagtatt ccctctactt
     1861 cagaaatggc atcgctgtta accacagccg gagcggagta caacatgaag ctggtgaacg
     1921 atatcaattt tgtagataaa tacaccactc cccgtaagca agcgctcaac ctgggtattt
     1981 acggagccga cctcaactat tgctccattt acgagcgcac caacgaaacc atgttctaca
     2041 tggaatgtac gcgctcactt gcagaaaaac tggggattga aaatgcaatt accgacgaaa
     2101 ccgtaacccg cgccgaagag aacatcgaca accgcgactc catgctcacg atcatttctg
     2161 atatgttcta tgaactggaa gaatacctgg cggaaaacga caaagacgaa ctctctgcat
     2221 tggtcattgg tgcaggatgg gttgaaggac tgtacctggc tacacaaacg gtaaacgaaa
     2281 agaaaccgaa ccaggacatc atgagccgta ttgctgaaca aaaactttca ttgggtaatt
     2341 tgatgaaatt tattgaacag tatatcgccg atgaagatgt gaaatcactg ttcgatgacc
     2401 ttacccgtat ctcaaaaatt tatgagggaa ttgtagtgga aaaacaagaa accaaagtga
     2461 gcaccgatag cgacggaaaa accgttatcg gaggaaccac caaagtggaa atgtcaacag
     2521 aacaattcaa agaacttcgt aaagtcgtta aggaaatccg cgacagttat actaaaatca
     2581 agtgatcatg aaattccgcc atatcctctt tcttgctctt gttgcttttc ttggcatggc
     2641 ccaagaagca gatgcacaat gcaaaagtta cgttaaacgc aactgtcgtc cgggattgac
     2701 tccctatctc cacaacggtc aggtcaataa tgcgatcctc gttccgggcg acaaagcaga
     2761 attgctgctt acttttttcg ccggtcagga ataccgttta atgatctgct ccatgcctgt
     2821 gcttggcgca gtaagtttcc gtgtgttgga ttccgatcgc aatcccattt acgaatcgaa
     2881 aaagaatccg agtcagaact acttcgattt taaagtagcc tctacccaac agctgattgt
     2941 agaaatcaac gtaccagaag caaaacccaa taatgatatc gttcccgaag gttgtgtttc
     3001 ggtattgata ggattcatta aaaaataatt ttatcccact tcatttaaag cgccctctaa
     3061 ggcgcttttt ttgtttcgtc taatctgttg aagactgttc aacaaagagt cctgaaaatc
     3121 ggttaaacga aattgcattc gaagacacgc taccccataa aacagaaaag tagcccgtaa
     3181 agagcactgc ccttctttct ttgcacctta accataaaca caaaaaaaga ccgtctctga
     3241 tggaggcagt ctctttagga ttattaagta cgctgttact cgaggatcag tttcttcaca
     3301 acagattgtc ctcctacatt caaacgaaca tagtaaactc ccgatgcaag atcgccacgt
     3361 ggaacagaaa gattgtgttc gccagaagaa agattcccca ggttacgtcc cattaccact
     3421 ttaccaagca tatcgaacaa ttcgatgcta actgtttcat tgctgttaag aacgaaaacc
     3481 agattagcca gatcacttga aggattgggg aaaatactga acgcaaaatc gttgtcggcc
     3541 tcttccacag caactgctcc tgaaagatta atatcgtcga tataaacgtt gtttccacct
     3601 cctgcttcaa aggagatctt gaacatgaaa ttaggaacca ggtaagccgc aaggttggta
     3661 acatctacct gcacccactg actcgaattt ggcgtaaacg ctgcagtggt attaggcgca
     3721 gtaggaagcg atgtgcttgc gatgttacgg cgcagctgcc atgtttcacc acaatccgaa
     3781 gaagcgtaaa ctttgatttt atctgtattg gtagatgaat tcttgtaagc gaaagcatac
     3841 ttgaaactca actgcattga accgtacata ctcatatcgt aagtaaccga ctgaatctcg
     3901 tcaaccgcac ctgctgattg ggcgctgttg tttaatcgga tgcattttgt tcctgtagta
     3961 gcagcaagac tgcttacttg ccatgcctgt ccgccatcaa aattgttgat gaaccaattg
     4021 gcattcggta ctgtggttgc gctttcaaac ccttcagaaa atggagtgcc caaaccgtta
     4081 tagggaagaa ctgtgatgta agaatttttg gtagctgttt gtgttacact tccgttgcca
     4141 acagtcaatg ataccgcata ggttcccggt gcgctgtaca caattccagt cggattctga
     4201 tctgtgcttg ttgccggtga tgctccggta aaggaccagc tccagttgct cggaccatgg
     4261 aatgaattgt ctgtgaaatc gatcgactga cccacacaaa ctacctgatt aaaggttgaa
     4321 aaatccgctg cacaaagatt atccacaccg gttacacctg catcaataat attctgtgcc
     4381 tgccaaagct gattgcgctg gccggttgta gaattcaatg cagcaatcat acgcgctttt
     4441 tgtccgttgg taaacatgcg tgaacaatac gaatactcca tatagttctg cacgttgtcg
     4501 atggtattac cacaggtagc accattaatg ttacacgtgt tccatcccat tgtttgaggt
     4561 gtatccgtta cattatcatc accgcaggaa acacccggat tattggtact cccccacaca
     4621 tgcgacaaat taagccagtg accaacttca tgcgtgaggg ttctgctgcg ctgcacagca
     4681 cctgtaccaa ttgaaccaac ataactatga ttcacccaaa taccatcgta tttcgcgggt
     4741 gtaaatccgt ttggtgtcat ggtataaccc gctgcaccat cagcgttttt acatacccag
     4801 atattgagat agtgattccg cggaaaacga acattgttgg tgttggttgg actgttgttc
     4861 aggttacggt tcacatcatc caccatcgga cgatctccgt cgttggtttt ggtggaataa
     4921 gtgcgcgtga taccacttac acaagcgcca agctcatcgt attgcgccag gcggaattcg
     4981 ataccaacat tcgcaattat gtttgcaaat gcagacacta ctgctgaagt atctgcgttt
     5041 tgtttattga aatcgcggtt aagtatgaat acagcatcct gcacctgcgc atctgaaatg
     5101 ttttctgtac caccctcgtg aacgatatgg aacacaatcg gaataacata aactgcagaa
     5161 cctccacgca tttccggcgt ttggttagcg atgatctgtt gaatttctgc ttcataagca
     5221 tcttcagcca gttgatgtgc ggcgttatag ccaacttcct gctttacttt ttcggtaaaa
     5281 cgatgctgac cacaaggttc aacatgttgt tccctgagtt gagcggataa tagcgtgctc
     5341 gcaaatacgg cacccaataa aagcattgtc tttttcattg ttcaaagaga gagttattga
     5401 atatagtccc tgagattcac cacgggttcc gtgagtgcac tgaggtcttt aaatatactc
     5461 aatattgctg cagtgacctg ctccggtcta tacagctttc cctcatcctt gtactcctga
     5521 aatctgagaa gcgatgaaaa atttccggga tcagaagaac gaatgtaggc ttgcatacca
     5581 gtatccacca ctccgggata aacggaaaac acacgtatat gatcaagctt aagttcttgt
     5641 aattctgcat ttaacacccg ggtgaacata tctacaccgg ccttgcttgc acaataggca
     5701 gcccaggatg gaatagggtt aattgcagcc cctgaactga tgtttagcaa taaacaggga
     5761 taatttttag tcctcgcctg atttaggaaa cggtcgcata tctgggtgag tgacaccaga
     5821 ttcaactgaa aaacctgctg gtaatgctcc tgtgataggt cgccggttcg atttatttca
     5881 ccgattgttc cggcattgtt aatgagtacg acgcgatctg catcatcagg aagttcaagc
     5941 ggcatattcg ccactgctcc cggatgagaa agatcgatgg aatggtgcgt gtaatcggga
     6001 tgatgaattt gattgctgcg actgtatcct gtaatttttg ccttgcccag caattcttcc
     6061 accagggatt ttccaatacc ggttccggtt cccgtgatga aataatggtc catcgtttac
     6121 tgattgatat caataaaccg cgcaacatcg tgtgatgtac cgaagatgat cagtgtatcg
     6181 gtatcgtaca ccacactttc tgatttggga acgccgagaa tgtgatcttc ttttaccaca
     6241 tccgctccgt cttttccatc gaaactacgc ttaatggtaa tcaatgtgag acggtatttg
     6301 tcgcgcaatc cgatatcgtt taaggtgcgg ttcacaatat ttttgggcgc acggatctct
     6361 gcgatttcgt aatcatcggg taattggaga aaagataaaa tacttggatt caacagacgt
     6421 tctgctacca cgaatgcaac ctcttcttcg ggtaagagaa tttcttttac gccgattttc
     6481 tcaagaatca ttttctgctg cggaccatgt gctcttgcga ttacgcgctt aatccccaaa
     6541 tcgatcaggt gagcgcaagt aagaatagtg gcttcaaaat tttctccgat ggcaacaaca
     6601 acgccatcca catccgaaat gttttgcgaa accaaggctt tatcgtcggt cgagtcgagc
     6661 gttgctgcgt aggcaacatc atcctttaca ttgtcaattt tttcttcgtc ctgatcaaaa
     6721 cagtacactt ccgctccttt ttgagccagt cgacgcgcaa tggaggaacc gtatcgtccg
     6781 actccaatta ctgcaaattt atttccgtgt aagggcatga tctaaaaagt taaatgataa
     6841 aaatgaaaag cgaattaacc caatgcttgt ttaagatcag caagtaaatc atcgcaatct
     6901 tcaacaccca cgcttaaacg caacaatgaa tctaccactc cggccttttc gcgctcttcc
     6961 tttggaatgg aggcatgcgt cattgttgca ggatgattga tgagtgattc tactccacca
     7021 agtgattctg ccaatgagaa gacattgaaa gaagaagcca gtttaaatgt ttcttcgatg
     7081 tttcccttat aaacgaaaga gatcattccg ccaaatccgc gcatttgctt tttggcaatg
     7141 gcatgattgg gatggtcttc aaatccgggc cagtacactt tttcaatttt cggatgcgac
     7201 cgtaaaaaat gggcgatctt ctccccattg gaacaatgac gttccatacg aaggtgaagc
     7261 gtttttattc cgcgtattac caaaaaagaa tccattggtc cgggagttgc accgcaggaa
     7321 ttatgaatga aagccagtcg ctcgaataat ttatcatcgt tcatcaccag cgcacccatc
     7381 accacatcgc tgtgtccgcc caggtatttg gtaaccgaat gcattacgat atgagctccc
     7441 agatcaagag gattttgcaa atacggtgag gcaaacgtat tatcgacagc caacagtaaa
     7501 ttcttctctt tcgcaattgc tgcacaagca gcgatatcaa tgatctgcat ggttggattg
     7561 gttggtgttt ccagccagat catttttgtg tttgcattga tgtaattacg gatgttttcc
     7621 gcattattca tggcaacaaa atgaaacttg attccgaatg gcgcgaaaac cttggtgaac
     7681 atgcggtacg aaccaccgta caaatcatca cctgttatca cttcatcacc aggctgcaac
     7741 aatttgatta ccgcatccat tgctcccatt ccgcttgaaa aacacaatcc gtgtttcgcc
     7801 ttttccaatg ctgcaatatt tttttcaagt gccgcacgtg ttggattttt tccacgcgca
     7861 tatgcatatc cctgatgttt tcccggagat tcctgaacaa aagtggaggt ctggaaaata
     7921 ggcgtcataa tagcgccggt agaaggatca ggttccattc ctgcatgaat ggcgcgtgtt
     7981 ccaaagccga gtttactggt gtctttcata tggcgaaatt atagaattcg ttcgactatt
     8041 acctgatttc cgtaagtaaa gtggcagaaa ctgaaaagag cggtgtgtat tccttcaggg
     8101 cttgcggggc ggagcaacgg tatcgctggt atgcgacaac caacccggaa taaccattgc
     8161 gccgataagc agatatgggt aataactgat taagcgccat aacactgcaa gcattccggc
     8221 cagaccaaag ggcacaaact cggcaagaaa tcctgaaaaa gcgaactccg caataccact
     8281 gcctccgggg gtaggactca ccatcaacaa cacccacatg ctgagttgtc gggcaaaaac
     8341 aataaactga tcggccatag gagcgaaggc catgatcaaa aagttgatga ccagaaaacg
     8401 acctgtccac gaaattaccg tcgccaaaaa tgcctgtaac caaaaggatc gggatttgcc
     8461 tcttaattcg gtagaagcaa ttacgatatc gtttccggta cgctctgctt tttctttcaa
     8521 acggcgcaaa aaaggaagcc tgcaaaaaaa caacaagagc gatcgcaatc cctgcggtac
     8581 aataaaaatt ccggcaaaga gtaaaacggt tatcgctgcg ataattccaa atgcaatcca
     8641 gaatacttcc aacacgccga attccattcc caaaaaatta ccggtgaatt ctaccggaaa
     8701 caaggatgca cttcctgcaa taagaattaa aacaggcaca atgagaatgt aaaaaagctc
     8761 atcgagtaaa gccgtgatca ataccagtgc agttgaccgt cccatattga tcttttccct
     8821 gttcaggata aacatcgcaa cagccgcacc gccaaccacg ccgggagtca tggcagaagc
     8881 aaactcccaa accataatca ccctgaacga ttgcctccag gataattcct tatcggtaag
     8941 gattcgaata cgccacatgt aagcagcatc gcgcaacacc atcataaagc aggcgagaag
     9001 aatacataag gaagaatacc aggtccagtc tatcgatcgc caactttcgg cgaatgaaat
     9061 tcttcggaaa tttcctccct cagcaaccgg cgaaaaatcg ttggcattac tgaaatcaac
     9121 gatgccatta cgattggtat cggtccagat gtaattcccc ctttcgcttt caacaaattt
     9181 ctcttctgct actgcattgc gcaccatcca aaaagcaacc gcaaggccca gcagcaaagg
     9241 cagcataata cgccagcggg aaaatgtttt tttgatgctt ggagcggatg ccatgaacct
     9301 tttcgctatc ttgtttgttc agaaggagac tattctttcg aaagatgaaa aaaatctacc
     9361 atttatccac ctgcagtacc tgtaaacgca tcctttctga actaaaaccg ggggctgacg
     9421 tggtattaca ggatatcaaa tcggacgctc ttactgaaac gcaagtggat gatatggcta
     9481 agctggccgg atcgtatgag gcgattttca gtcgtgtcgc aatgaaatac agggccctgg
     9541 gtctcgataa gaaaactctt tccgaaaaag attacaaaaa atacattctg gaagaataca
     9601 cttttcttcg tcggcccgta gtgcttgctg ataaccggat ttttattggc aactcaagaa
     9661 agatcgttga atcggccgct gaggcattac gcaaataatc tttcacacgc ctatatcttc
     9721 cactcaggag atctgccctg acaaaaagaa atcctacatc ggtatacaaa gtttatttgt
     9781 acgaattaaa ggggttgttt attttggcag ctaaaccgca ggctgctcat gattaaaaga
     9841 caactcaacc ggagttaccg catcagcaaa tatgtttttt acagagagac gcttgttgac
     9901 tccaaagaat tgttttggtc gtttattggt tctttcattg gaattggctg cattgctttt
     9961 ttacaaagtc tgtacctcac agaaactgaa aacgtgtttt tgatcggatc gtttggagca
    10021 acaggcgttc taatatacgg agcgatacag agtccgctcg cccagccaag aaatctgatt
    10081 ggcggacatc tcatcagtgc atttatcgga gttaccgtgt ataaaatcat tccggatatt
    10141 atttggttat cggcaccact tgccgtgtcc ttatccattg cagcaatgca gtttaccaaa
    10201 acactgcacc ctcccggagg agcaacagct ttaattgcgg taatcgggac agaaaaaata
    10261 aaagcgcttg ggtatctgta tatagttact cctgttcttt cgggagtttt aatcatgctc
    10321 attgttgcgt tggtctttaa caatatcacc ccgatgcgtt cgtatccggt taaaggaaga
    10381 tttgcacgga tggtaaacgt atttggaaaa aacaaaaaca agggtaaata atgccggatc
    10441 gtatccatta aggaaaaaat caaaattcca attacccggc acgcttttac cttaatttta
    10501 gcgccagaac aaaaaacatg tcacgcagcc tacttggccg tggaaagcta cgacgaccgc
    10561 atcgatccgg tgggcgcctg cgtaggtatg aaaggaagcc gtatccatgg catcgtgcgg
    10621 gagctgaaga atgagaatat agatgtgatc aactggacaa acaatatcca gttgttgatc
    10681 cagcgttcgc tcacaccagc caaaatcacc agcatcgaac tcgatacgga aaagaaacat
    10741 gcgaatgttt accttaagcc cgaccaggtg tcactggcta tcggccgccg tggggtaaac
    10801 atcaaacttg cctgtgaact cacaggattt gaaattgacg tcttccgcga taacgaaggg
    10861 gaagaagagg aatttgatat cgacctcgaa gaatttacag acgagatcga accatggatc
    10921 atcgatgaac tcaagcgagt gggttgcgat acggcccgtt ccgtactcga cctgaccgcc
    10981 gaggaactcg agcgccgcac cgacctggaa aaagaaacca tcacggaagt gcgccgtatc
    11041 ctgaaagaag aatttgagcg ggattgatga taggaatgac tggctgaaac gcgcgtggag
    11101 acgagagcat attgattcat aataaaacag aaatctggat ttcgccaagg cggaaccaca
    11161 atacaacaga atgtcagaag taaacacacc caggttgatg gctgcggcca aggagtttaa
    11221 catcggtacc aacacactga ttgaattcct tgtgtcaaag aatttcagca gcgatgacct
    11281 taagccgacc agccggctga cggaacccat gtaccgcgta ttgcaggccg aattccagca
    11341 agacaaggta gccaggcaaa aagcggaaca gatagacctt ccgaaagggg tttccgcgag
    11401 tgagcaaaaa aagaagaaag acgaagaaga tttgtcgatc cgcaaaaagg aaaaagaaaa
    11461 agaagaaaag gccaaagctg aagagaagaa gcctgaacct gttgaggaag cgccggtagc
    11521 cgcgcctgct gcggagaaag aagagcccgt agctgaaaca gtttccggta atgaggttac
    11581 aaaaattgaa gcgcctgaac tcgaaggccc gaagattgtc aagaagattg accttgacgc
    11641 cattgattca tctacgaggc caaagaaaac taccaaaaaa gcggcacccg aagaagtgac
    11701 acctg
//
LOCUS       DAOMYG010000029        11461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1874_length_11461_cov_8.621690, whole genome shotgun sequence.
ACCESSION   DAOMYG010000029 DAOMYG010000000
VERSION     DAOMYG010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 11461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11461
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1874_length_11461_cov_8.621690"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..897
                     /locus_tag="PK610_01780"
     CDS             85..897
                     /locus_tag="PK610_01780"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ34680.1"
                     /translation="MKTVLITGTSSGIGKSTVKLFHQKGWNVIATSRNPEKEKDLKED
                     DRLMITALDVTKPSGIQEAISKGIARFGKIDVLVNNAGYGLVGPFEAATDEQIRRQFD
                     TNVHGIFNTTQLILPHFREKKSGVIINVASVGGQITFPLYSLYHSTKWAVEGFSESLH
                     YELREHGIRIKIIEPGPIKTDFYDRSQDLMKKDGLNAYDAFVSKTFPVLQKFGANASG
                     PEIVAAKIYRAATDGRKKLRYPVGGGAGAVLFLRRILPFAVFRSIVRWVTKS"
     gene            1006..3081
                     /locus_tag="PK610_01785"
     CDS             1006..3081
                     /locus_tag="PK610_01785"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HRJ34681.1"
                     /translation="MKSVFSFLLGVFAFGNAVAQGDFHCGADELRITTLQKNPAIADA
                     VNRREAILEAHTQNYIQQENRGGGGPYIIPVVFHVIHNFGPENISDAQIQDAIRVVNR
                     NYRKLNADTVDIIQPYKALAADCNIELRLAQKDPNGNCTRGINRIVSTTTYTGGHEVK
                     QLIQWDPTKYLNIYIVSNAAGLAGHAVWPADADTIPEWDGIVISHDYVGSTGTSNAVR
                     SVVLSHELGHYLNLHHIWGGNNVPGFYYLPVGQAGNCAYDDLVSDTPNTIGWNTCNLN
                     HTSCGNTQADNVQNFMDYAYCARMLTHGQAARMHACLNSPIAGRNNLWSPANLIATGT
                     DGNSVLCQADFSADKRLICAGESITIYDNSYHGVTIRTWTFPGGTASSLSDSVVTVSY
                     PSAGNYTVSLSVGNGSTSTSSSLSNYITVMQNPGIPGQIIEHFEGITALPNSDWHPVD
                     PQGDGGFELATSTGYLSSTCVKVNNYTLNEGYVDELILSPRNISGFPSIQISFMFAFT
                     QKDSAFVNDKLRVYVSNDCGANWALRRTLAASSMITAPFTSLPFIPGDETQWMLNSIT
                     NIPNSFITDDFMVKFTWESDGGNNFYLDNINIAANLSVEEKENKISAILYPQPNNGSF
                     AIEWNTFNFNKGYLEIYSATGQKLSEQHIQSGNSRAEVQLSNLAKGFYFVRLNLDENQ
                     LVLPLIISE"
     gene            complement(3135..4004)
                     /locus_tag="PK610_01790"
     CDS             complement(3135..4004)
                     /locus_tag="PK610_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34682.1"
                     /translation="MFVVGLAMFSKVAENAHCAGTHHNMATVVDDARLYSEQQKGITF
                     LFTSAYKEVKQCRKSANSSSLKMDGASPVVPSVTIKGFNVENDKTLKALNNSLTGFQL
                     VVNGSGGTVEIEGSPDVEKLSPYDREMYEAITNPSKSLTIDQETTSIIGDLKKLRKNS
                     YSISVKLSVLFADNKSYRIKEISRALFGGALREFNTGSYQAGLDFQVSSFYALYNMEA
                     FYAYTGNNKRVRVNGFYTGVDYERTIVLGKVYYKQVSEETTMNVFAVNVHIGEATRFY
                     RENTNDIPVVLTK"
     gene            complement(4143..4730)
                     /locus_tag="PK610_01795"
     CDS             complement(4143..4730)
                     /locus_tag="PK610_01795"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRJ34683.1"
                     /translation="MKRFFDIISSLIVLLIGLPFFLLLALAIVIDSRGGIFYRQIRVG
                     RNEKDFYLYKFRSMVSNADKKGQLTVGASDSRITRVGKFIRKFKLDEFPQLINVIKGE
                     MSIVGPRPEVPKYVKMYNNEQRKVLSVRPGLTDYASLEYINENEILGKAENPEQLYIQ
                     EIMPAKLELNLRYIAEKSLLTDLKIIFRTIGRIFK"
     gene            complement(4727..5950)
                     /locus_tag="PK610_01800"
     CDS             complement(4727..5950)
                     /locus_tag="PK610_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HRJ34684.1"
                     /translation="MSLLPFSPPRMDEKITEAVKEVLLSGWITTGPKTKLFEKKLTEY
                     TGSKATLCLNSATAGLEIMLRWFGVGDGDEVIVPAYTYSATANVCMHVGARPVLVDIN
                     PHDFNISVEAVRKAITPRTKVIMPVDFAGMPCDMDELNAIVNEENIRKLFKPGNEIQQ
                     QLGRILILSDAAHSIGAQYKGKRTGSLCDVAVFSFHAVKNLTTAEGGAVALNLPAPFD
                     NEAVYKYLCIKTLHGQNKDALAKTQRGAWRYDILEPGYKCNMMDIQAAIGLVELERYD
                     EDNLVRRKQIAERYIKALSVDPRFELPVFETADKISCYHLFPLRIRGCNEEQRDAIID
                     AIFARDISVNVHFIPLPLLSFYKSEGYVMSDYPVAYDNYSREISLPLYYNLSDEDVDR
                     VIAAVFASVEEVLKA"
     gene            complement(5970..6182)
                     /locus_tag="PK610_01805"
     CDS             complement(5970..6182)
                     /locus_tag="PK610_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRJ34685.1"
                     /translation="MHQAQLLTYLKLTKKKVGLLINFNEVHLKTASDASKTDPCKVVN
                     SPLLALPSIHTSVNPIPPSVKPINHL"
     gene            complement(6098..6379)
                     /locus_tag="PK610_01810"
     CDS             complement(6098..6379)
                     /locus_tag="PK610_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRJ34686.1"
                     /translation="MGPGLLESIYEECLCMELTERGFTYVRQRPVYINYKGESTNLHF
                     RLDLLIEDQIVVELKAVMSLSEFTKLNYSPTSNSQKRRSVCSSISTKST"
     gene            complement(6556..7797)
                     /locus_tag="PK610_01815"
     CDS             complement(6556..7797)
                     /locus_tag="PK610_01815"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRJ34687.1"
                     /translation="MAERFINILRDPFLLPWLVLVFVLPLFPVAVPWAAMLVVVSSLF
                     RFREFRLDQQKLRSLGPWILLYLLYGIGLFYTENIERGWISMERKLSLLVFPLLFIFL
                     PAFDVHKRNLLQWLLVAGVILNSMLSYSEAASCYFDSGSRDCWHSSALSFQLHPTYNA
                     FYALTALSVVIYSLLQYGKSIKTRLLLLSLGVWLMAFVVLLASKAGLLGLTILLFAAL
                     IYAVVRSGNYRWLSWGAGGFLVIIIAIRLFSPITFERFTATIGSSQMSEEELFRTYAS
                     TTESNAVRRMIWIVSGEIIADHPLGVGTGDVYPSLEKKYTERQMTGALERQLNAHSQY
                     IQTTIALGYPGLLVLLFALISGILYAWKRNPLQLIFLMLFAVNIAFESMLEVQAGVVF
                     FAVMVGVFTDGGDTSTGSVQV"
     gene            complement(7790..8458)
                     /locus_tag="PK610_01820"
     CDS             complement(7790..8458)
                     /locus_tag="PK610_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ34688.1"
                     /translation="MKKVFWLPNGVNVRLFKNDSEGAKKWRSEQGYTDNDVLFLYAGI
                     IGYAQGLDVILNAAKRTKENPNIKYILLGSGPEKERLLELAASLHLQNVKFLEPVEKK
                     QMPAIVSAIDAAVIPLRKLDLFLGAIPSKLFENLALEKPLVLGVDGEARELFIEKGRG
                     GLYFEPENDEELANAIQRLAHDPELRKQLGKQGAEYVKEYFDLDKIAAKFSSILIENS
                     AKNG"
     gene            complement(8455..9027)
                     /locus_tag="PK610_01825"
     CDS             complement(8455..9027)
                     /locus_tag="PK610_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34689.1"
                     /translation="MKILILTQYFPPEVGAPQNRLFELAVRLKEQGNEVTILTAMPNY
                     PKMVVHEGYRRKWYKREEIAGLTVHRSWIFVSQRKSIFFRLLNYFSFVFSSFWVGLFR
                     IRRQDVLLVESPPLFLGKTAFLLSRLKRAKMIFNVSDLWPESAEKLGLVKIGFSSGYP
                     ISWRRFCTVARGSLPVRPRVSLKIFNIVFR"
     gene            complement(9024..10124)
                     /locus_tag="PK610_01830"
     CDS             complement(9024..10124)
                     /locus_tag="PK610_01830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ34690.1"
                     /translation="MTDPSDLRILILADVNSAHVQRWASGLAKRGLQIAVWSFDIPAN
                     DWYSKHRIKVYAPDSPSFFGKFRYFFAEKKAQEAIDDFRPHVVHAHYASSYGMIGRKT
                     GFHPFFISVWGSDITHFPNGMIRRSIMRKNLDAADRIFATSHMLTQILEHHWGVDAVR
                     MPFGVDTEKFQEKKFNHYFPSSSVVVGTVKALESIYAIDFLLAAFSMVQKRRPDLPLR
                     LLIAGDGSLRNNLIRQASRDCIPETVKFVGKVPYDAVPLIHQEIDIFMNVSQYESFGV
                     SVLEASSCGKPVIVTETGGLPEVVEDDETGYLVSVGSIVQMADAIERLAENVALRSTM
                     GANGRFFAASKYNWDSIIEDMVGHYRNVVDFQ"
     gene            complement(10108..11025)
                     /locus_tag="PK610_01835"
     CDS             complement(10108..11025)
                     /locus_tag="PK610_01835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRJ34691.1"
                     /translation="MQSGHKVSVIIVNFRVKDVLRDCLLSLRSHAVSGMEVLIVDNAS
                     HDGSVEMVRAEFPEFQLIASNSNMGFSPANNVGMDACTGDLILLLNPDTIVEPGALQH
                     WVDLHSVTKAGISGPKLLNADGSLQVSAWRIPGLLSSLLELFFLHRLFSTGNYPAETF
                     NSDFSPECLSGAALLFDRAIYAKVGGLDPELFWMEDVDLCFRVSKAGYAVRYFHEPRI
                     THLGGQSSKSNLNRVISNQLISRLKFASKHRNFLHVFVLHLVVFCHILSRVLLFHLLL
                     PIAGLARAKAYRFTFVKYLRYLFLHDRSI"
     gene            complement(11242..>11461)
                     /locus_tag="PK610_01840"
     CDS             complement(11242..>11461)
                     /locus_tag="PK610_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34692.1"
                     /translation="IADALVEGKSGILFPAEATAAAYADFFAAHFLQIENHGKWMVSS
                     RDVYEQTHNWERWGDEMEKMIKAFCQHQ"
BASE COUNT         3462 a         2628 c         2342 g         3029 t
ORIGIN      
        1 tgttaaattt tagaacagct tacgtaaagc aaacaacacg cattcaatga actgtatcat
       61 tacattttta aactgacagc ctaaatgaaa accgttttaa tcacaggaac atcatccgga
      121 attggaaagt caacagtaaa acttttccac caaaagggat ggaacgtcat tgcaacctca
      181 agaaaccctg aaaaagagaa ggatctaaaa gaagatgatc gtttgatgat caccgcattg
      241 gatgtgacaa aaccatcagg cattcaggaa gctatttcca aaggaattgc gcgtttcgga
      301 aaaattgatg tgctggtaaa taacgccggt tacggattgg ttggtccttt cgaagccgcc
      361 actgatgagc agattcgcag gcagtttgat accaacgtac atggaatatt taataccact
      421 caactaattc ttccacattt cagggaaaag aaatccgggg tgatcattaa tgtagcttcg
      481 gttggcggac aaataacttt tccgctttac agtttatacc attccacaaa atgggcagtt
      541 gaaggattca gcgaatcact tcactacgaa ttgcgtgaac acggaatacg aatcaaaatc
      601 attgaaccgg gaccgataaa aacggacttc tacgatcgtt cacaggactt gatgaaaaaa
      661 gacggtctaa atgcttacga tgcatttgtg agcaaaacat ttccggtatt gcaaaaattt
      721 ggagcgaacg ccagtggtcc tgaaattgtt gcggcaaaaa tttacagagc agctaccgat
      781 ggacgaaaaa aattgcgcta tcctgtagga ggtggcgccg gggctgtgtt gttcctccga
      841 cgaattttgc cgtttgcagt attcagaagc atcgttcgtt gggtaaccaa gagttgaaat
      901 ctgatttctt tgtatcagta aatagctttc tactaaatcg atcaatgttt ttttcaaaaa
      961 aaacagtaca atttctttag tatattgtgg actgaattca aatttatgaa atcagttttt
     1021 tcttttctgt taggtgtttt cgcttttgga aacgcggttg cacaaggtga ttttcattgt
     1081 ggtgctgatg aattgcgcat cacaaccctt caaaaaaatc cggcgattgc ggatgcagtc
     1141 aaccgacgcg aagcaattct tgaagcgcac acccaaaact atattcagca ggaaaacaga
     1201 ggtggtggtg gtccatacat tatcccagtt gtgtttcacg tcattcacaa tttcggaccg
     1261 gagaacatca gtgatgcaca gattcaggat gcgattcgtg tagtaaaccg caactacaga
     1321 aagttaaatg cagatactgt agatattatt cagccctaca aagcgcttgc ggcggattgc
     1381 aatatcgaat tacgactcgc acaaaaagat cccaatggaa attgcacccg aggcatcaac
     1441 cgaatcgttt ctaccacaac atataccgga ggacacgaag taaaacagtt gatccagtgg
     1501 gacccgacaa aatacctcaa tatttatatt gtttccaatg ctgcaggatt agcaggacat
     1561 gcagtttggc cggcagatgc agatacgatc ccggagtggg atggtattgt gatctcgcat
     1621 gattatgttg gaagtaccgg aacatccaac gccgttcgat cggtagttct ctcgcacgaa
     1681 cttgggcatt atttaaatct tcatcacata tggggaggaa acaatgtccc cggattttat
     1741 tatttacccg ttggtcaggc cggaaattgc gcctacgatg atctcgtgag cgacacaccg
     1801 aacacgattg gttggaatac ctgcaacctc aatcatacct cctgcggaaa tacgcaagcc
     1861 gacaatgttc agaatttcat ggactatgcc tattgcgcac gcatgctaac tcatggtcag
     1921 gcagcccgta tgcatgcgtg tctgaattca ccaattgcag gtcggaataa tttatggtcg
     1981 cctgccaatc tgattgcaac cggtacagac ggaaattcag tgttatgcca ggccgatttt
     2041 tctgctgata agcgtttgat ttgtgccgga gaatccatca ccatttatga taattcgtat
     2101 catggtgtta ccatccgcac ctggaccttt ccgggaggaa cagcctcatc gctcagtgat
     2161 tctgtggtaa cagtaagtta tccctctgcc ggaaattata cggtttcgct aagcgttggc
     2221 aatggcagca catcaacatc ttcaagcctg agcaattaca ttaccgttat gcaaaacccc
     2281 ggtattcccg gacaaatcat cgaacatttc gaaggcatta ctgcactacc caattccgac
     2341 tggcatccgg tggatccaca aggagatggc ggatttgaac tcgccacaag tacaggctat
     2401 ttaagttcca cctgtgtgaa ggttaacaat tacacattga atgaaggata tgtcgatgag
     2461 ttgatcttgt cgccacgaaa tatttcaggt ttcccttcta ttcaaatctc attcatgttt
     2521 gcatttacac aaaaagattc tgcatttgta aacgacaaat tgcgcgtgta tgtaagcaat
     2581 gactgcggcg ccaattgggc attacgcaga acgctagcag caagctctat gatcactgca
     2641 ccatttactt ctcttccctt tattccaggt gacgaaaccc aatggatgtt gaattccatt
     2701 accaatatcc caaacagttt tatcaccgac gattttatgg taaagtttac ctgggaaagt
     2761 gatggaggaa ataattttta cctcgacaac atcaatatag ctgccaatct ttccgtggaa
     2821 gaaaaagaaa acaaaatttc ggcgatcctt tacccacagc ccaacaacgg atcatttgcc
     2881 attgaatgga atacctttaa tttcaataaa ggctacctgg aaatttattc agccaccgga
     2941 caaaaactta gtgaacaaca catccaatcc ggtaattcaa gagcagaagt tcaactatcc
     3001 aatctggcaa aaggatttta ttttgttcga ctgaacctgg atgaaaatca attggtattg
     3061 ccactcatca tcagcgaata attaatattc caaaattcca tcctgtgcac tgaaaatggc
     3121 ggcaaacaaa tcggctattt tgttagtaca acaggaatgt catttgtgtt ttcccgataa
     3181 aaccgggtcg cttcgccaat gtggacgttt acagcgaaaa cattcatcgt tgtttcttca
     3241 ctgacttgtt tgtaatagac ctttcctaaa acaatggttc gttcataatc aactccggta
     3301 taaaatccgt ttactcttac ccgcttattg tttccggtgt aagcataaaa agcttccata
     3361 ttataaagtg catagaaact actcacctga aaatccaatc ctgcctgata acttccggta
     3421 ttaaactccc gcaaagctcc gccaaaaaga gcccggctga tttcttttat ccggtaactt
     3481 ttattgtcgg cgaacaaaac agaaagtttc acactgatcg aataactgtt cttgcgcaac
     3541 ttttttaaat cgccaataat acttgttgtt tcctgatcga tggttaatga ttttgaagga
     3601 ttggtaatcg cttcgtacat ctcacggtca taaggagaaa gtttttcaac atccggactt
     3661 ccttcaattt caacagtgcc acccgaaccg tttacaacca gttgaaatcc tgttaaagaa
     3721 ttattcaacg ctttcagtgt cttatcgttt tctacattga atcctttaat ggtaactgaa
     3781 gggacaacag gtgatgcacc atccattttt aaagaagaac tattggcgga ttttcggcat
     3841 tgctttactt ccttataggc agatgtaaaa agaaatgtaa ttcccttttg ctgttctgaa
     3901 tacaagcggg catcatcaac aaccgttgcc atattgtgat gtgttccggc gcaatgagcg
     3961 ttctcagcaa ctttagaaaa catcgccaga cctacaacaa acaaaatatt taatttaaac
     4021 ttccgcagat caattcagat tacactaatg gagaacgaag aatgactatg gttctgtact
     4081 aaaatactga attcccggaa agcatccaac agaattcact tcaactccac caaacgaatt
     4141 gcctatttaa aaatacggcc tattgttctg aaaattattt tcaggtcagt cagtaaactt
     4201 ttttcggcaa tatatcgcag attcaattcc agtttagccg gcatgatctc ctgaatataa
     4261 agttgttcag ggttctcagc cttgccgagg atctcgtttt cattgatgta ttccagcgag
     4321 gcataatcgg taagtccggg tcgaacagat agcactttcc gctgctcatt gttgtacatt
     4381 tttacatact tcggcacttc gggacgtggt ccgacgatgc tcatttctcc tttgatcaca
     4441 ttgatcagct gaggaaattc atcgagtttg aattttcgga taaacttccc tacacgtgta
     4501 atccgtgaat cggatgcacc cacggtaagc tgaccttttt tatcggcatt gctcaccatt
     4561 gaacgaaatt tgtagaggta aaaatccttt tcatttcttc ctacgcgaat ttgtctgtaa
     4621 aaaattccac cacgtgaatc gatcacaatc gccaaggcaa gcaggagaaa aaaaggcaat
     4681 ccgatcagta aaacgatcag cgaagaaatg atatcgaaga aacgtttcat gccttcagta
     4741 cttcctccac cgaagcaaac actgcagcaa ttacgcggtc gacatcctcg tcgctgagat
     4801 tgtaatacaa tggcaaggaa atttctcttg aatagttatc gtaggccacc ggataatcgc
     4861 tcatgacata tccctccgac ttgtaaaaac taagcaaggg cagcggaata aaatgcacgt
     4921 tcaccgaaat atcgcgggcg aaaatggcat cgatgatagc atcgcgttgt tcttcattgc
     4981 atccgcgaat acgaagcggg aacaaatggt aacaggaaat tttatcggca gtttcgaaca
     5041 ccggtaattc gaatcgggga tcaaccgaaa gcgccttgat gtaccgttcg gcaatctgct
     5101 ttcgtcgcac caggttgtct tcgtcgtatc gttcaagctc caccaatcca attgcagcct
     5161 gaatatccat catgttgcac ttgtaaccgg gttcaagaat atcgtaccgc catgcaccgc
     5221 gttgggtttt ggcaagggca tctttatttt gaccatgcaa ggtttttatg cagaggtatt
     5281 tgtacaccgc ttcattatca aaaggcgcag gcaggttaag tgccacagct cctccttcgg
     5341 cagtggtgag gttttttaca gcatgaaaag aaaaaacagc aacatcacac agtgatcctg
     5401 tacgttttcc tttgtattgc gctccgatag agtgtgcagc atcgctgagg ataagaatac
     5461 gtcctaattg ctgctgaatt tcgtttcctg gcttaaatag tttacgaatg ttttcttcat
     5521 tcacgatggc attgagctca tccatatcac agggcatgcc tgcaaaatca acgggcatga
     5581 tgaccttggt acgtggcgta atggctttgc ggacggcttc tacagagata ttgaaatcgt
     5641 gggggttgat atcaaccagc actggcctgg caccaacgtg catgcagaca ttggcggtgg
     5701 cggaataggt ataggcggga acaatcactt catcgccatc acctactcca aaccaacgga
     5761 gcatgatttc gagtccggcc gttgcagaat tgagacacag tgtggctttg cttccggtgt
     5821 attcggtcag ttttttctcg aagagtttgg tttttggacc ggtggtgatc cagccggaaa
     5881 ggaggacttc ctttactgct tcggtaattt tttcgtccat acgcggtgga ctgaaaggga
     5941 ggagactcat gctccaaatg tagggagttt tacagatgat tgatgggttt cacagatgga
     6001 gggatggggt ttacagaagt atggatggat ggaagggcta gaagaggtga atttacgacc
     6061 ttacatggat cggttttcga ggcgtcggat gccgttttta ggtggacttc gttgaaattg
     6121 atgagcaaac cgaccttctt ttttgtgagt ttgaggtagg tgagtagttg agcttggtga
     6181 attccgataa ggacatcaca gcttttaact ctacaacaat ttgatcttct attaaaagat
     6241 caagcctgaa atgaaggttg gtgctttcac ctttgtagtt aatgtaaaca gggcgctgcc
     6301 ttacgtaggt aaaccctctt tctgttaatt ccatacacaa acattcttcg tatatcgatt
     6361 ccaacaaacc gggtcccatt tcggtgtgaa cagtgtaagc agcatccaat atttctttcg
     6421 acagcggata aatttggcag gtaaagtatt catgctgcaa acatcctgcg aacacttttc
     6481 ttagtcaagc gaattcaaac caataaacat attcattgaa cagttcgatc aagttaactc
     6541 ttctcttgcc ttccttcaca cttgcactga gcccgtcgaa gtgtcccctc catccgtaaa
     6601 cacccctacc atcactgcaa aaaacaccac tcctgcctgc acttcaagca tcgattcgaa
     6661 agcgatgttc actgcaaaaa gcatcagaaa aatcaattga aggggattcc tcttccacgc
     6721 ataaaggatg ccggatataa gtgcaaaaag taaaaccagt aagccgggat aaccaagtgc
     6781 aatagtggtc tggatgtatt gcgaatgcgc attcaattgc cgctcgagtg caccggtcat
     6841 ttggcgttcg gtgtacttct tctccagaga aggatagaca tcacctgttc ccactccaag
     6901 gggatgatcg gcaatgatct cacctgaaac gatccagatc attctgcgca ctgcattgct
     6961 ttcggtagtt gaggcatacg tgcggaacaa ttcctcctcc gacatctgcg aagaaccaat
     7021 ggtagctgta aaacgttcga aagtgattgg ggaaaacaaa cggatcgcaa taatgataac
     7081 caaaaaacca cctgcacccc aactgagcca gcggtaattt cccgagcgta caacagcata
     7141 gatcaaggca gcgaacaaaa gaatagtcaa acccagtaaa cctgccttag aagcgagcaa
     7201 aaccacaaaa gccatcagcc aaacaccaag tgataaaagc agcaatctcg ttttgattga
     7261 tttcccgtat tgaagaaggc tgtaaatgac aacagaaagt gcggtgagcg cataaaaagc
     7321 attgtaggta ggatgcaatt gaaacgacag tgcactcgaa tgccagcaat cgcgcgaacc
     7381 ggaatcaaaa tagcaacttg ccgcctccga ataactgagc atactgttaa gaattactcc
     7441 ggccaccagc aaccattgca ggaggtttcg tttatggaca tcaaatgcag ggagaaaaat
     7501 gaataacaaa ggaaagacaa gcaatgacaa tttccgctcc attgatatcc acccgcgctc
     7561 gatgttttcg gtatagaaca atccgattcc gtacaaaaga taaagcaaga tccagggacc
     7621 cagcgaacga agtttttgtt ggtctaaacg aaactcccta aaacgaaaca aggaactgac
     7681 caccaccagc atcgcagccc agggaactgc caccggaaac aaaggcaata caaataccag
     7741 caccaaccag ggaagcaaaa aaggatcgcg caggatgttt ataaaacgtt cagccatttt
     7801 tagcactgtt ttcaatgaga atggaactga attttgcggc aattttatcg agatcaaaat
     7861 attctttcac atactccgct ccttgtttac ccaattgctt gcggagttca ggatcgtgag
     7921 caaggcgttg aatcgcattg gcaagttctt catcgttttc cggttcgaaa tacaatcctc
     7981 ccctaccctt ttcaatgaac aattcgcgtg cttcaccatc gactcctaaa acaagtggtt
     8041 tttccagcgc cagattttca aacagtttgg aaggaatcgc ccccagaaat aaatcgagtt
     8101 tacgcagagg aataactgca gcatcaattg cagaaacaat agcaggcatt tgttttttct
     8161 cgaccggctc aaggaatttt acattctgta aatgcaatga agcagcgagt tcaagcagtc
     8221 gttctttttc cggtccgctc cccagcagga tgtatttaat attcggattc tcttttgttc
     8281 gtttcgctgc attcaaaatc acatccaagc cctgagcata tccgataata ccggcataca
     8341 aaaacaacac atcgttatca gtatatcctt gttcgcttct ccatttcttt gctccttcac
     8401 tatcgttttt aaacaatcga acattcactc cattgggcaa ccaaaatact tttttcaccg
     8461 gaaaacgatg ttgaatatct ttaacgatac cctgggtctg accggtaatg atccacgagc
     8521 gacggtacaa aaacgcctcc agcttatagg atatccagag gaaaaaccga tttttacgag
     8581 tccgagtttt tctgcactct cgggccagag gtcgctcaca ttaaatatca tttttgcacg
     8641 cttcaatcgc gaaagcagga acgctgtctt ccccaaaaac aagggaggag attccaccag
     8701 cagcacatcc tgccgacgga tacgaaacaa tcccacccag aaggaagaaa acacaaacga
     8761 aaaataattg agcaatcgga aaaagatact cttgcgctga ctcacaaaaa tccacgaacg
     8821 atgaacagtt agtccggcta tttcttcacg cttgtaccat tttctccggt aaccttcatg
     8881 caccaccatc ttcggataat tgggcatggc ggtaagaatc gtcacttcat tcccctgttc
     8941 cttcaaacgc acagccaact caaacaatcg gttttgcggc gctccaactt cgggaggaaa
     9001 gtactgggta agaatcagga ttttcattgg aaatcgacca cgttacggta atgtccaacc
     9061 atatcttcga tgattgaatc ccaattgtat tttgaagctg caaaaaatct tccgtttgct
     9121 cccatagtcg agcgcaatgc aacattctcc gcgagtcgct ctatggcatc ggccatttgt
     9181 acaatagatc ctacagaaac aagatatccg gtttcatcat cctctacaac ttccggtaaa
     9241 ccgccggtct cggtcacaat tacaggtttt ccgcaggaag aagcttccag aactgatact
     9301 ccaaaacttt cgtactgact cacattcata aaaatatcaa tctcctggtg aatgagcggc
     9361 acagcatcgt aagggacttt tccaacgaac tttaccgttt cgggaataca atcgcgtgag
     9421 gcctggcgga tcaaattatt tcggagtgat ccatcacctg caatcaacaa tcgtaagggc
     9481 aaatccggcc ttcgcttctg aaccatggaa aatgcagcga gaaggaaatc aattgcgtaa
     9541 atagattcaa gcgctttaac cgttcccaca acaacagacg atgaaggaaa ataatgattg
     9601 aactttttct cctgaaattt ttctgtatca actccaaaag gcatccgaac ggcgtctact
     9661 ccccagtggt gctcgaggat ttgtgtaagc atatgactgg tagcaaatat tctgtcggca
     9721 gcatccaggt ttttccgcat gatcgatctt cttatcatac cgtttggaaa atgggtgata
     9781 tcacttcccc aaactgaaat aaaaaatgga tgaaatcccg ttttacgtcc aatcatccca
     9841 taactgcttg cataatgcgc atgaacaaca tgtggacgaa aatcgtcgat agcctcctga
     9901 gcttttttct cagcgaagaa gtacctgaac tttccaaaaa atgaaggtga gtctggcgca
     9961 taaactttaa tgcggtgttt gctgtaccaa tcattggcag ggatatcgaa tgaccaaacc
    10021 gcaatttgca atccccgctt tgccaatccg cttgcccagc gttgcacatg cgctgaattc
    10081 acatcagcaa gaatcaatat tcgtaaatca gatggatcgg tcatggagga aaagataacg
    10141 caaatatttt acaaaagtaa atctgtacgc tttggcacgg gccagtcctg caatcggcag
    10201 cagcaaatga aacaacaaga cgcgacttaa aatgtgacaa aaaacaacaa gatgcaaaac
    10261 aaaaacatgc aggaagtttc tgtgtttgga ggcaaatttc aaacggctga tcaactggtt
    10321 gctgatcaca cgattcaaat tgcttttgga actttgtcca ccaagatgag ttatccttgg
    10381 ctcatgaaaa taccttactg cgtatcctgc ctttgaaaca cgaaagcaaa ggtccacatc
    10441 ttccatccag aacaactccg gatcgagtcc tcccaccttt gcataaatcg ctctatcgaa
    10501 taacaaagca gcgcctgaca gacattccgg ggaaaaatcg gaattaaatg tttcggcagg
    10561 ataattcccg gttgaaaaca aacgatgcaa aaaaaacaac tccagcaagg aagacaataa
    10621 tccgggaatt ctccaggcgc tcacctgcaa actaccatcc gcattcagca gttttggtcc
    10681 ggaaattcca gccttggtaa cactgtgcag atccacccaa tgctgcaaag caccaggctc
    10741 tacaatcgtg tcaggattca gcaagagaat caaatcgccc gtacaagcat ccattcccac
    10801 attatttgca ggggaaaagc ccatattgga attggatgcg atcaattgaa actccggaaa
    10861 ctctgcacga accatttcaa cactgccatc atgagaagca ttatccacaa taagcacttc
    10921 cattcccgaa accgcatgcg agcgtaagga aagcagacag tcacgcaaga catcctttac
    10981 ccggaagttt acaataataa cactgacttt atgcccagac tgcattcaac aaatatactt
    11041 actccgatga tgatattaca gtagcaaaca aagaagagtg aacagcgaaa agtgaacagt
    11101 tttcggcgaa taatgaccgg atgattgcaa aaccctaaac gaggtattgg aaatcaaata
    11161 ttacagagta cacaaggata ttcgaagttc attcctttac caataatcaa agacaagccg
    11221 agctgaaatg ctttgaaaat tttattggtg ttgacaaaat gccttgatca ttttctccat
    11281 ttcatcaccc catctctccc agttgtgcgt ttgttcgtac acatcgcgcg aagagaccat
    11341 ccacttcccg tggttttcaa tttgtaaaaa gtgagctgca aaaaaatcgg catacgccgc
    11401 agcagtagct tctgcaggaa agagaattcc actttttcct tctacaagag catcggcaat
    11461 a
//
LOCUS       DAOMYG010000030        11301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_1910_length_11301_cov_8.907789, whole genome shotgun sequence.
ACCESSION   DAOMYG010000030 DAOMYG010000000
VERSION     DAOMYG010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 11301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11301
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1910_length_11301_cov_8.907789"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PK610_01845"
     CDS             <1..637
                     /locus_tag="PK610_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34693.1"
                     /translation="IMPFWVQCQSAEKGKLFIDTGGGLGVYHGFSIFSPPENAGINAA
                     AFSGNLRINYFVKPTISTGIEIRHLVYPSNESDTNRVIASASGTGILLTAENVFFNRK
                     KFHAFVGAGLGVYSFAYERYERDSTQTISENLGQIKARGTSIYLGTGFRWCFSKHLGL
                     NFRAGYSIYPLRVNEYRFNGNVQEEMDYRKSTEVILNFRGVEGQLGLSVFF"
     gene            complement(839..2110)
                     /locus_tag="PK610_01850"
     CDS             complement(839..2110)
                     /locus_tag="PK610_01850"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRJ34694.1"
                     /translation="MYNYSPQFLDSLSPDGSIVLKRTSDTIDISRDYESFYCMKGEVT
                     NSQFREFRDWVRDSVIRRVLSLKDPNVFLLQSRNEDGSRRLNWKVKVRFNDSLISNQI
                     KDLYLPDHERYYRRKEFDYKKLQYIFQIGHEEKNVPVVPDSLSWVYDFQYMEYALLDG
                     FWLNLMDPMVNMYFWHPAYDDYPVVGITFEQAAAYCQWKTKMMNNKLEKNGITVKVSI
                     PTAVESDFVHLFLQSQTAHDPKYNLEEMFTDVPLSVYDVISFRDLAILQAHQFSDSIK
                     LISPFVPNVIINRWLNPYWYNDCVFGGEFIRKNSSGKKESEKVENYYDNVSEWIDTDF
                     SGLRVEVHERRRKMQLLYFLENSKNVSFTERAEGVFKGSKMIRGVNFLDVRNGSKDWL
                     IPSHTFLSPDSVRSTVGFRCVMRVEEKKKSR"
     gene            complement(2364..2867)
                     /locus_tag="PK610_01855"
     CDS             complement(2364..2867)
                     /locus_tag="PK610_01855"
                     /inference="COORDINATES: protein motif:HMM:NF019241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRJ34695.1"
                     /translation="MMSLTEQMVKEYRRRVFGESIPRIRKCLNLLSEVEIWKQPNSEI
                     PAVGNLVLHLCGNARQWMLSGLFNIDDERNRDAEFNSRGGWTKAELHALLDQLENDVD
                     INLECVSEKLLHEIHKVQVFKESGMSIMIHVIEHFSYHTGQISLLTKYYTELDLGYYS
                     GLDLNKK"
     gene            complement(2885..5866)
                     /locus_tag="PK610_01860"
     CDS             complement(2885..5866)
                     /locus_tag="PK610_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRJ34696.1"
                     /translation="MRGILFFLLLHLSGVLSAQHDHLVVQQGHTDEILFVSVDPKGKT
                     YATASRDKTVKVWDLRSGKLIKTVTGFSFYVTKVGYSPEGGFLYMRGDLGTCKVFSTG
                     NFEEVTTKFYDDFFPITQLNFGVNDDMLLFGIKGSAFIVNTKSWNKHLLSGRVAGEVD
                     HIVFDPGTKKVTVRAKGGNPTDIDVASILGKENFSIDRKPSEFYPYSAEVNSTIVTIK
                     DASGSVIAIRKQPRVSIPSNAVFSPDNQSLAICFESSNNVLIFSIRHNSVQRIPTKFA
                     EVRAVVFSPDGKRILIGDGAQMISCNMDGSERKSHYEHNAAITCADISKDNKLLLSGS
                     VDGSLSLYDLVQGKLLASVKAGGVVTSLSIHPKGKLAAVAEDGHVTVFKLPALKEEKV
                     AIKGTATRSTDFSGDGKFLVAQDAKTKRVMLYETGKEEPAKSFRDPSEHKTRSEAAIR
                     KQLFPVAFSNTGKFLISMGEEWVMHLRVQPDYSVSELKGHSSWANSFHFSSDDKLLVS
                     TSDDGSVRLWDTDKKQNIATMVFNDKDFVVANSENYYFTSKGGYDLVGFYKGGNVFPF
                     EQFDLVLNRPDKVLSTLGSKNVALIAALEKTWMKRIEKSGLQKVEAGGNPPVVSILNR
                     DKIGIQTKTKDLKLSLRATSQGAAIDRILIWINEVPVFGKNGISLKKKGLTDHTQDII
                     IPLAAGSNTIDVAVMNDKGIESGRERVITDFSDNSKPTLYFAGLGISEFAQSGFNLKY
                     PTKDVNDIDALVKSLKGTVFADVQSKVFTNEKVTVSALGDIRSFFRKAGRNDMVILFF
                     AGHGVLDAGFNYYLSSYDMDFASPAAKGLSYESLESLLDSIAPLKKLFLIDACHSGEV
                     EKDEMILASVSTAEKNGDVGFRAVGNGFVRKDAMGLRSSSELLKEIFADVRRGTGATI
                     ISSAGGAEFAMESSEWKNGLFSYCLLNGLKNHEADADDNGKVQVSELLNYVMNAVREL
                     SSGRQNPTTRAENVSLDFELW"
     gene            6034..7845
                     /gene="typA"
                     /locus_tag="PK610_01865"
     CDS             6034..7845
                     /gene="typA"
                     /locus_tag="PK610_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRJ34697.1"
                     /translation="MQKLRNIAIIAHVDHGKTTLVDKMLLVGKLFKEHETPGELILDN
                     NDLERERGITILAKNVSVKYKDYKINIIDTPGHADFGGEVERVLNMADGVLLLVDAFE
                     GPMPQTRFVTSKALAMGLKPILVVNKVDKPNCTPEEAYEAVFELMMNLDATDEQLDFP
                     VVYGSAKNNWMGIDWKKPTDNIEALFEMIISHIPEPKVEVGTPQLLITSLDFSQYIGR
                     FAIGRLQRGELKSGQQVALVKRDGTITKQRIKDLFVFEGFEKRKVDNVQAGDICAVVG
                     IEGFEIGDTIADSEKPEALKTIDIEEPTMSMLFTINNSPFFGKEGKFVTSRHLKERLE
                     KELEKNLALRVAETNSADSFNVSGRGVLHLSVLIETMRREGYELQIGQPQVILKQIDG
                     IKCEPIEELTIDLPEEHAGKAIEMVSTRKGEMLNMSPKGGRMVLQFLIPSRGLIGLRN
                     NMLTATAGEAVVTHRFKEYAPFKGEIPGRQNGSLISMENGDAIAYSINNLQDRGTFFI
                     DVGEPIYEGQVIGENSRPGDLVVNVTKTKKLTNMRASGSDDKMRIAPPKRFSLEEALE
                     YIQGDEYVEVTPTNIRLRKVILSESDRKRASNTKLQL"
     gene            complement(7895..8806)
                     /locus_tag="PK610_01870"
     CDS             complement(7895..8806)
                     /locus_tag="PK610_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRJ34698.1"
                     /translation="MDINFTKAAELKGHAGPVYALCAGSREGTFFSGSSDGFVAEWDL
                     VSFMPEKFSVKIGQPVYALEHILEFHLLVAGTGSGAFHVIDLKEKKEVRHIVQHSSSV
                     FDVRYNSKQKHLYTVSADGHFCVWEVPTFRLLIDYPICSEKLRHIAISPSGDKIALAC
                     GDHIVRVFETEFYNPIRDLKAHSMSVNSVFFTNENSLISGGKDAFLHWWNSESEIPYL
                     SVPAHNFAIYTIVGSPDGKLFATGSRDKTVKIWNAETNDFLLRIERKSHLAHTASVNR
                     LYWDKFTGLLVSAGDDRTVFCWRKSVE"
     gene            complement(8821..9171)
                     /locus_tag="PK610_01875"
     CDS             complement(8821..9171)
                     /locus_tag="PK610_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34699.1"
                     /translation="MTERLRIDEKVLGYRRLQGKHWFYSPDMYSWSGNAILFKQSDRS
                     TGIRDRNRRLIFENDILEDNVLGFLEIAFDDALGRLFVAALQENGLYSLDWLAERSGT
                     NSFVWKSFSFLQEP"
     gene            9413..11284
                     /gene="htpG"
                     /locus_tag="PK610_01880"
     CDS             9413..11284
                     /gene="htpG"
                     /locus_tag="PK610_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008991520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRJ34700.1"
                     /translation="MTTGKINVQTENIFPIIKKFLYSDHEIFLRELVSNAVDATRKLK
                     TLASIGEFKGELGDTRIRISIDKDKKTLTISDRGIGMTKEEVEKYINQVAFSGAKEFV
                     EMYQDKAQGIIGNFGLGFYSSFMVSRQVEIHTRSYKPDTTPVIWQCEGSPDYTLLEGN
                     RTERGTDIILHIAEDSEEFLDEFRIKNILTKYCKFLPVEIAFGKRKEKTTDENGNENE
                     IEVDDVINNPEPAWTKAPASLKEEDYKSFYNELYPFAEEPLFHIHLNVDYPFNLTGIL
                     YFPRLKNNIEVQKNKIQLYSNQVFVTDQVEGVVPEFLMLLHGVIDSPDIPLNVSRSYL
                     QSDANVKKISAHITKKVADKLEEMFKKDREDFQKKWDDIQVFIHYGMLTDEKFLERAQ
                     KFALLKNTAEKYFTLDEYTEKIKPLQTDKNNRTVYLYASSIDAHHTYIQNAKERGYDV
                     LLLEGPLVSHLITRLEQKLENASFVRVDADTIDKLIQKEDALPSKLNEKEQESIKPVF
                     EDCVDKTKYTVVFESMSEKDVPVMVTQTEFMRRMKEQQAMGGGGGFYGMFPEMYNLVI
                     NSNHPLTSKILNETDNDRKKQMGRQVVDLALLQQGLLKGEELSAFLKRSVEMIEKMG"
BASE COUNT         3585 a         2465 c         2132 g         3119 t
ORIGIN      
        1 tattatgcct ttttgggttc aatgccaatc tgcagaaaaa ggaaaattat tcattgacac
       61 aggaggtgga ttaggggtgt atcacggttt ttccattttc agtccaccgg aaaatgcagg
      121 aattaatgcc gctgcatttt ccggcaacct tcgaatcaac tattttgtga aaccaacaat
      181 ttcaacagga attgagattc gtcatttggt ctatccctcc aatgaatccg acaccaaccg
      241 tgtgatcgct tctgcttcag gaactggaat tttacttact gcagaaaatg ttttcttcaa
      301 cagaaaaaaa tttcatgcat tcgtaggtgc aggattaggg gtgtacagtt ttgcatacga
      361 gcgttacgaa cgtgatagta cccaaactat ttccgaaaat ttaggtcaga tcaaagccag
      421 gggaaccagc atttacctgg gaaccggatt tcgctggtgt ttcagtaaac acctaggttt
      481 aaatttcaga gcgggatact ccatttaccc attgcgtgtt aatgaatacc gatttaacgg
      541 caacgtacag gaagaaatgg attaccgaaa atcaactgag gttattctaa attttcgggg
      601 agtggaagga caattgggtt tgagtgtatt cttttaagtt ggcaggacaa ccagaaactt
      661 catacgttcg ccaagaaaaa aaaatttctt gatactcatt tagtagactt agtgtcctag
      721 tggtaaaaaa actaccacaa ctcaaaatcc aatgaaacat tctcagcccg tgttgtaggg
      781 ttctgccgtc ctgatgaaag ttctcgaaca gcattcatca cataattgag caattcactt
      841 acctggactt tttcttttcc tccactcgca ttacgcaacg aaaaccgaca gtagacctta
      901 cagagtcagg actaagaaac gtatgggatg gaattagcca gtcctttgat ccgtttctta
      961 catccaaaaa attaacacca cgtatcattt tgctgccttt aaaaacccct tcagcacgct
     1021 ctgtaaatga tacattcttt gaattttcaa gaaaatacag caattgcatt ttccttctcc
     1081 tctcatgaac ctcgacccga aggcctgaaa aatctgtatc tatccattcg gatacgttgt
     1141 cataataatt ttcaactttt tcagattcct tttttccgga tgagtttttt cgaataaatt
     1201 ctccaccaaa aacacaatca ttataccaat aaggattcag ccacctgtta atgatgacat
     1261 ttggcacaaa agggctaatc aattttatgc tatctgaaaa ttgatgagcc tgaagaatag
     1321 ctaagtctct aaaagaaata acatcataaa cggatagtgg aacatcagta aacatttctt
     1381 ccaggttgta tttaggatcg tgtgctgttt gggattgcaa aaacaaatga acaaaatcag
     1441 attcaactgc cgttggaata gaaaccttta ctgttattcc attcttctcc aatttattat
     1501 tcatcatttt agttttccat tgacaatatg ctgccgcctg ttcaaacgta attccaacca
     1561 ccggataatc atcatatgcc ggatgccaga aatacatatt taccatagga tccattaaat
     1621 tcaaccaaaa gccatccaga agtgcgtatt ccatgtattg aaaatcatat acccatgata
     1681 aagaatcagg aacaaccgga acattttttt cttcgtgtcc gatttgaaaa atatactgta
     1741 attttttata atcgaattct ttccttctat agtagcgttc atgatcaggc agatataggt
     1801 ctttaatttg gttcgatatc agagaatcgt taaatctcac cttaaccttc caattcaacc
     1861 tccgagaacc atcttcattc ctggactgaa gcagaaatac attgggatct ttcaaagaaa
     1921 gtacccttct gataactgaa tcacgaaccc aatctctgaa ttcccggaat tgggaattgg
     1981 ttacttcgcc tttcatacaa taaaagcttt cgtaatcccg gctgatgtcg atagtatcag
     2041 aggttctttt taatacaata cttccatccg gtgaaagtga atcaagaaac tgaggagaat
     2101 agttatacat catggaagca ggaaaaaaat tccgttcctt tttcccttta agtaaaccag
     2161 gagaaatttt ttgccaaccc ggaaaaacgc ctttataatc gttttgctgc gtgtgtagga
     2221 gaaattttgt agaatattga atcgatctcc attcaagcat tctgacagga aatgcaaaat
     2281 ctgacagact ataataagtt gaattggcag gagaaaaaat ttgcataaaa accccaacca
     2341 aaatgcatct tgttaatctc atactacttc ttgtttaaat ccaatccact ataataacct
     2401 aaatccaatt cagtataata tttagttagc aaactgatct gtccggtatg gtaggagaaa
     2461 tgttcaataa catgtatcat aatactcatt ccactctcct taaaaacctg tactttatga
     2521 atttcgtgta atagcttttc cgaaacacat tcaagattga tatcaacatc attttctaac
     2581 tgatccaata aagcgtgtaa ttctgccttt gtccagcctc ccctggaatt aaattctgca
     2641 tctcgattcc tttcatcatc aatattaaat aatccggaga gcatccattg tctggcattt
     2701 ccacataaat gaagaaccaa atttcccaca gcagggattt ctgaatttgg ttgtttccag
     2761 atttccactt ccgataacaa attcagacac tttcggatac gtggaatact ttcgccaaat
     2821 acacgacgac ggtattcctt caccatttgt tctgttaatg acatcatttc aaattacaat
     2881 cttattacca caactcaaaa tccaatgaaa cattctcagc ccgtgttgta gggttctgcc
     2941 gtcctgatga aagttctcga acagcattca tcacataatt gagcaattca cttacctgga
     3001 cttttccgtt atcatcggca tctgcttcat gattctttag tccattcagc aaacaatagg
     3061 aaaacaagcc atttttccac tcgctactct ccatcgcaaa ttctgcccct ccggcgctgc
     3121 taatgattgt tgcaccggta ccgcgacgaa catcagcaaa aatttcctta agtaattcgg
     3181 atgaagaacg taatcccatg gcgtcttttc gcacaaaacc attacctaca gcacgaaatc
     3241 caacatcgcc gtttttttct gcagtagaaa cagaagcaag gatcatttca tctttttcaa
     3301 cttcgccgct atgacaggca tcaatcagaa acaacttttt cagaggagca atgctatcca
     3361 gaagggattc aagtgattcg taagacaatc ccttagcagc aggagaggcg aaatccatat
     3421 cataagacga caaataataa ttaaaaccgg catccaacac accatgtcct gcaaaaaaca
     3481 aaatcaccat atcatttcgt cctgcctttc gaaaaaagga acgaatatca ccaagtgcag
     3541 atacggttac tttttcattg gtaaacactt tgctttgtac atctgcaaaa actgtccctt
     3601 tgagtgattt caccaaggca tcaatatcat tcacatcttt ggttgggtat ttcaggttga
     3661 atccagattg tgcaaattct gagataccca atccggcaaa ataaagtgta ggttttgagt
     3721 tatcggaaaa atcggtaatc acacgttctc ttcctgattc aatcccctta tcgttcatca
     3781 cagcaacatc aatggtatta cttcctgcag ctaaaggaat gattatgtcc tgtgtgtgat
     3841 ctgtgaggcc ttttttcttc aaggagattc catttttacc aaatacagga acttcattga
     3901 tccaaattag aattctgtcg atcgcagcgc cttgagatgt tgcacgaagg gagagtttga
     3961 gatccttagt tttggtctga attccgattt tatcgcgatt gagaattgat accacaggag
     4021 gatttccacc tgcttcaact ttttgcaatc ctgatttctc gatccgtttc atccaggttt
     4081 tctccagcgc agcaataagt gcaacgtttt ttgatcccaa tgttgataaa actttatccg
     4141 gacgattcaa taccaaatca aattgttcaa atggaaaaac atttcctcct ttgtaaaaac
     4201 caaccaaatc gtatcctcct tttgacgtga aatagtaatt ctcactgttg gcaaccacga
     4261 aatccttatc gttgaatacc atggtagcga tattttgctt tttatcagta tcccaaagcc
     4321 ttacagatcc gtcatccgaa gtggaaacaa gtaatttatc atcactactg aagtgaaacg
     4381 aattggccca gctggaatgt cctttcaatt cagatacaga ataatcgggt tgaacccgca
     4441 aatgcatgac ccactcctca cccatgctga tcaggaattt cccggtattg gaaaaagcaa
     4501 caggaaacaa ttgttttctg attgcagctt cacttcgcgt tttgtgctcg gatgggtcgc
     4561 ggaatgattt tgcaggttcc tcttttcctg tttcgtacaa cattaccctt ttggtctttg
     4621 catcctgagc caccagaaat tttccatcgc ctgaaaaatc tgtgcttcgt gttgctgttc
     4681 ccttgatcgc aaccttttct tctttcagtg caggcaattt aaaaacggta acatgaccat
     4741 cttccgcaac tgcagcaagt tttccctttg ggtgaatact caacgacgta actacccccc
     4801 ctgcctttac gcttgcaagc agtttaccct gcactaaatc atacaaactc aatgaaccat
     4861 ccacacttcc cgataacaat aatttattat ccttgctgat atccgcacag gtaattgcag
     4921 cattgtgttc atagtgactt tttctttcgg aaccatccat attgcaggat atcatctgag
     4981 cgccatctcc aattagaatt cgttttccat cgggagaaaa tacaacagca cggacttccg
     5041 caaatttggt tggtatgcgt tgaacggaat tatgacgaat ggaaaagatc agaacattgt
     5101 tgcttgactc aaagcaaatg gccagtgatt gattatcggg actaaaaacc gcgttggaag
     5161 gaatagagac acgtggttgc ttacgaatgg caatgacaga accggaggca tccttaatgg
     5221 ttacaatggt agaattaacc tcagcactgt aaggataaaa ctcggaaggt tttcggtcga
     5281 tggaaaaatt ttccttgcca aggatggagg caacatcaat atccgttgga tttcctcctt
     5341 tggcacggac tgttaccttt ttagtacccg gatcgaaaac aatatgatcc acttcaccgg
     5401 caacgcgtcc ggataacaaa tgtttattcc acgactttgt atttacaata aatgcagatc
     5461 cttttatacc gaacaacaac atatcatcgt ttacaccgaa attcaattgt gttattggga
     5521 aaaaatcatc gtaaaatttt gtggtgactt cttcaaaatt accggtcgaa aaaactttgc
     5581 atgtacccag atcaccacgc atgtataaaa atccaccttc gggactataa ccgacctttg
     5641 taacataaaa tgaaaatcct gttactgttt taatcaattt tccactacgg aggtcccaaa
     5701 ccttaacggt tttatcacga cttgcagttg catacgtttt gcctttggga tcaacagaaa
     5761 caaaaagaat ttcgtcagta tgtccctgtt gtactacgag atgatcatgt tgtgctgata
     5821 atacaccgga taaatgaaga agaagaaaga ataaaatacc gcgcataact gttgttttgg
     5881 ctaaagataa ttgactctat cgaatcatcg aataaggtaa acacctgaca ttaaacccct
     5941 aaggaatcac cttttatcca gcgatttaca caaaacatag tggaaatgag gaataaaagc
     6001 gtacctttgc acccttattt cagcacctta ttcatgcaaa aactcagaaa catcgcgatc
     6061 atcgcgcacg tcgaccacgg taagacgaca cttgtagaca aaatgctcct agttggaaaa
     6121 ctgttcaaag agcacgaaac ccccggagaa ttaattcttg acaacaacga cctagaacgt
     6181 gaacgtggaa ttaccattct tgctaaaaac gtatctgtaa aatacaagga ttacaaaatc
     6241 aacatcatcg atacaccggg tcacgccgac tttggtggtg aggttgaacg cgtgcttaac
     6301 atggcagacg gagtattgtt gcttgttgac gcctttgaag gtccgatgcc acaaacacgt
     6361 tttgtaacat caaaagcact tgcaatggga ctaaaaccca ttcttgtagt gaacaaagtt
     6421 gacaaaccca actgtacacc cgaagaagct tacgaagcgg tattcgaatt gatgatgaat
     6481 cttgacgcta ccgatgagca gttggatttt cctgttgttt acggttcagc aaagaacaac
     6541 tggatgggaa ttgactggaa aaaaccgact gacaatatcg aggcgctttt cgagatgatc
     6601 atttcgcata ttcctgagcc aaaagttgaa gttggaacac cacagttact gatcacttca
     6661 ctcgactttt cacaatacat cggtcgtttt gcgattggtc gtttgcaaag aggagaattg
     6721 aaatcaggtc agcaagtagc acttgtaaag cgcgacggaa ccatcaccaa gcaacgcatc
     6781 aaagatcttt tcgtttttga aggattcgaa aagcgtaagg ttgataatgt tcaggcagga
     6841 gacatttgtg ctgttgtagg aattgaagga tttgaaattg gcgacactat tgccgattca
     6901 gaaaaacctg aagcattaaa aacaatcgat atcgaagagc ctaccatgag tatgcttttc
     6961 accatcaaca attctccatt cttcggtaaa gaaggaaagt ttgtaacctc acgtcacctc
     7021 aaagagcgtc ttgaaaaaga acttgaaaaa aatcttgctc tccgtgttgc agaaaccaat
     7081 tcagccgatt cgttcaacgt atccggacgt ggagtactgc acctttctgt tctcattgaa
     7141 accatgcgtc gcgaaggtta tgaattacaa attggtcagc cacaggtaat cctcaagcag
     7201 atcgacggta tcaaatgcga accgattgaa gaattgacca tcgaccttcc tgaagaacat
     7261 gcaggaaaag ccatcgaaat ggtttcaact cgtaaaggag aaatgttgaa tatgagtcca
     7321 aaaggcggac gcatggttct ccagtttttg attccttcac gcggattaat tggattgcgt
     7381 aacaacatgc tcactgctac tgcaggagaa gctgtggtta ctcaccgctt taaagaatat
     7441 gctccgttta aaggagaaat tcccggccgt cagaatggtt cattgatctc aatggaaaac
     7501 ggtgatgcca ttgcttattc cattaacaac ctgcaagacc gcggaacctt ctttatcgac
     7561 gtaggtgaac ctatttacga aggacaggta atcggcgaaa acagccgtcc cggtgacctc
     7621 gtagttaacg ttacaaaaac caagaagctt accaacatgc gtgcttcagg ttcagatgat
     7681 aaaatgcgta ttgcacctcc caagcgtttc agcctggaag aagcactcga atacattcag
     7741 ggagatgagt atgtggaagt aacacccacc aatattcgtc ttcgtaaagt aattctcagc
     7801 gaaagcgacc gtaagcgcgc ttcaaacacg aaacttcagc tttaatctga ttttacatta
     7861 atggaaagtc ccggcagagg tgtcgggact ttttttattc tactgatttt ctccagcaaa
     7921 aaaccgtacg atcgtcgcct gcagacacca ataggccggt gaatttatcc caatacaatc
     7981 tattcacgga agcagtgtgt gcaagatgtg atttacgttc gatgcgcaat agaaaatcat
     8041 ttgtttccgc attccaaatt tttacggtct tgtccctact accggtagca aacaattttc
     8101 cgtcgggaga accaacaata gtgtatatcg caaaattatg ggcgggaaca gataaatatg
     8161 gaatctctga ttcggaattc caccaatgaa gaaaggcatc tttacctcca cttatgagtg
     8221 aattctcatt tgtaaaaaaa acactgttga ccgacattga atgtgctttc aaatccctta
     8281 ttgggttgta aaattcagtt tcaaaaaccc gtacgatatg atctccacaa gccagtgcaa
     8341 tcttatctcc ggaaggagag atcgctatat gtcgcagttt ttcagaacag atcggataat
     8401 caatcagcaa gcgaaaggtg ggcacttccc aaacacagaa atgaccatcg gcagaaaccg
     8461 tgtacaagtg tttttgtttg gaattgtaac gtacatcgaa aacagaagac gaatgttgta
     8521 cgatatgccg gacttccttt ttttctttca ggtcgataac atgaaaagcg ccacttcccg
     8581 tgcctgcaac cagcaaatga aactcaagga tatgctcaag ggcatatact ggctgaccaa
     8641 ttttcacgga aaacttttcc ggcataaaag aaaccagatc ccactctgcc acaaaaccat
     8701 cactgctacc cgaaaagaaa gtcccctctc tgctacccgc gcacaaggca taaacaggac
     8761 cggcatgtcc tttcaattca gcagctttgg taaaattgat gtccacccgc taaagataca
     8821 tcaaggttcc tgcaaaaagg agaatgattt ccagacaaat gaattggtgc ccgatcgttc
     8881 agctaaccaa tcgagtgaat acaagccatt ttcctgtaag gcagcaacaa acaaacgtcc
     8941 caatgcatca tcgaaagcta tttccagaaa accgagtaca ttgtcctcca gaatatcatt
     9001 ctcaaaaatc aaacgtcggt tgcggtcgcg aatgcccgta ctgcggtcgc tttgcttaaa
     9061 aagaatcgca tttccactcc aggaatacat atccggcgaa taaaaccaat gcttcccctg
     9121 aagccggcga tacccaagca ccttttcatc gatccgcagc ctttctgtca taattaccac
     9181 aataacagta aaatcaggtg aattgttgca cccgaaatcg acattttggc agaaaatacc
     9241 gaatggcaaa agaattgaaa tgatagtggg cagtgatagg tgaacagtga acggtgaaca
     9301 gtgttctgaa gttctttgta attacttttt accacaccag gaacaaacac atgctaaaaa
     9361 ccacttacgg ataactgata actaataacc aacaacacat actccaccag ctatgacaac
     9421 aggaaaaatt aatgttcaga cggaaaacat tttccccatc atcaagaagt ttctttattc
     9481 tgatcatgag atctttttac gcgagttggt ttccaatgcg gttgatgcaa cgcgcaaact
     9541 gaaaacactt gcttcgattg gtgaattcaa aggtgaattg ggagataccc gaattcgcat
     9601 cagtattgac aaggacaaaa aaacactcac gatttctgac cgtggtattg gaatgaccaa
     9661 ggaagaagtt gaaaaataca tcaaccaggt tgctttttcg ggcgccaagg aattcgttga
     9721 gatgtatcag gacaaagctc aaggaatcat aggtaatttc ggactcggtt tttattcttc
     9781 gttcatggtt tcacgtcaag tggaaatcca tacccgctcg tacaaacccg acacaacacc
     9841 agtaatctgg cagtgtgaag gatcgccgga ttataccctt ttggaaggca accgtacaga
     9901 aagaggaacg gacatcatcc tgcacattgc agaagattcg gaggagtttc tggatgaatt
     9961 cagaataaaa aacattctta ccaaatattg caaattcctt cctgttgaaa ttgcgtttgg
    10021 aaaacgtaaa gaaaaaacaa cggatgaaaa cggtaatgaa aacgaaattg aagtagacga
    10081 tgtaatcaac aatcccgaac ccgcatggac aaaagctccg gcttcgctaa aggaggaaga
    10141 ttataagagt ttctacaatg aattgtatcc ttttgccgaa gaacctttgt ttcacatcca
    10201 cctgaatgtt gactacccct tcaatcttac cggaatactt tattttccgc gactgaaaaa
    10261 caatattgaa gttcagaaaa acaaaattca actttacagt aatcaggtat ttgttaccga
    10321 ccaggttgaa ggtgttgtgc cagaatttct gatgctactg cacggtgtga tcgattcgcc
    10381 ggatatccca ctcaacgttt cccgttcgta tttgcagagt gatgccaacg taaaaaaaat
    10441 atcggcccac atcactaaga aagtcgcaga caagcttgaa gaaatgttca aaaaagaccg
    10501 cgaagatttc cagaagaaat gggatgatat tcaggtcttc attcattacg gaatgcttac
    10561 ggatgaaaaa ttccttgagc gtgcgcagaa gtttgctctt ttgaaaaata cagcggaaaa
    10621 gtattttacg cttgacgaat acacagaaaa aatcaaacct ctgcaaacag ataaaaacaa
    10681 tcgcacagtt tatctctacg cctccagcat cgacgctcac cacacctata ttcaaaacgc
    10741 aaaagaacgc ggttacgatg tattgctgtt ggaaggaccg ctggtgtctc accttatcac
    10801 aaggctcgag caaaaactgg aaaatgcatc ctttgtacgt gttgatgccg acaccattga
    10861 caaactcata cagaaagaag acgcactgcc gtctaaactg aatgagaaag aacaggaaag
    10921 catcaagccc gtttttgaag actgcgtaga taaaaccaaa tacaccgttg tatttgaaag
    10981 catgagtgaa aaagacgttc cggttatggt tacacaaact gaattcatgc ggaggatgaa
    11041 agaacaacaa gctatgggtg gcggtggagg attttatgga atgttccctg agatgtacaa
    11101 cctggtgatc aattcgaacc atccgctgac ctcaaaaatc ctcaatgaaa cagacaatga
    11161 ccgcaaaaag caaatgggtc gtcaggtagt tgaccttgca ttgcttcaac aaggcctttt
    11221 gaaaggagaa gagctcagtg cgtttctgaa acgatcagta gaaatgatag agaaaatggg
    11281 ataaataaac ccaactgaac c
//
LOCUS       DAOMYG010000031        10919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2009_length_10919_cov_9.716679, whole genome shotgun sequence.
ACCESSION   DAOMYG010000031 DAOMYG010000000
VERSION     DAOMYG010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10919
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2009_length_10919_cov_9.716679"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1000)
                     /locus_tag="PK610_01885"
     CDS             complement(65..1000)
                     /locus_tag="PK610_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018945090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ34701.1"
                     /translation="MMDILRAENIVKRYRNHTALSGVNIAVPEGSIFGLLGPNGAGKT
                     SLIRIINQITGPDEGTVYFRDEKLSPHHLSNIGYLPEERGLYKKMKVGEQSLYLARLK
                     GMSKQEATRQLQIWFEKFEISGWWNKKIEDLSKGMAQKVQFITTVMHQPKLLILDEPF
                     SGFDPINADLIKKEILRMKENGTTIIFSTHNMGSVEEMCDEIALINNSKVILDGKMSD
                     IRKRFRSQVYEVEFNGNMIAFANALWAGFELLEREQVEPNHFRSRVKLLGNNTTNNLL
                     SALIPHVEIRSVREIIPSMNDIFIEVVQSQNQNHG"
     gene            complement(997..2148)
                     /gene="dnaJ"
                     /locus_tag="PK610_01890"
     CDS             complement(997..2148)
                     /gene="dnaJ"
                     /locus_tag="PK610_01890"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRJ34702.1"
                     /translation="MSKRDYYEILGVSRNAEADEIKKAYRKLALKFHPDKNPGDKEAE
                     EKFREAAEAYDALGDPEKRRRYDQFGHAGMGAGAGGYGGGGMNMDDIFSQFSDIFGGA
                     FGGGFSGGGGRGRRVQKGSNLRIKVKLTLEEIAKGVEKKIKVNKLNNCRNCNGTGAKN
                     SNAVRNCSTCGGTGHVTRVTSTFLGQMQTTQVCPTCHGQGTVVTEKCGGCNGEGTTRE
                     EEVISINIPAGVEEGMQMAVNGKGNAVRNGIPGDMIVVFEELPHDHLQRDGHNLHYDL
                     HLNFADAALGADCEIPTLEGKAKIHIDPGTQSGKILRLRNKGLPVLDSYQKGDLLVTI
                     NIWTPQKLSKEEKATLEQFRGSENFSPSPSKKEKSIFKRWKEMFTGHGD"
     gene            complement(2160..2726)
                     /locus_tag="PK610_01895"
     CDS             complement(2160..2726)
                     /locus_tag="PK610_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRJ34703.1"
                     /translation="MSKEETTNKETMNENENGNENGNGNENGNGNGNESPTVEEKTEE
                     TTDPVEEMNKKYILLYAEFDNYRKRTAREKADLIKSGGSDVIKSILPVIDDLERAIRA
                     NETATDLEIVKEGFRLIHHKFMNIMQAKGVKAIDILGQEFNADISEAIANVPAPNDEM
                     KGKVMDVVENGYTMNDTVIRFAKVVVGQ"
     gene            complement(2831..3388)
                     /locus_tag="PK610_01900"
     CDS             complement(2831..3388)
                     /locus_tag="PK610_01900"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRJ34704.1"
                     /translation="MNRINILLGASALLFFTLGIFRFGGSPSSVESSSLQILEVGSTA
                     PAIELPNPKGKTLKLSSIKGKYVLIDFWASWCGPCRKENPNLTEAWAKYSKAKFKDAS
                     GFEIFSVSLDTQKEAWTKAIANDKLSWKYHVSDLKGWNSPINAVYGIKSIPMNFLIDP
                     SGKIVATNLRGLELHKAIDAYVKSF"
     gene            complement(3629..4315)
                     /locus_tag="PK610_01905"
     CDS             complement(3629..4315)
                     /locus_tag="PK610_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRJ34705.1"
                     /translation="MRCIIVDDDELSRNIMEDLVREHEHLELVKTCSSAIEAFNVLKT
                     EEVDLIFLDVDMPKMSGMDLIRSLEVLPQVILITSHAEYAAESYEYSVTDFVVKPISH
                     GRFLKAVEKAQKNLSRPNFSSETSKTIFIKSDSRLVQVQKSNILYIEALGNYVNVYTA
                     GGKFIVLSTMKDIESRLTSPDFMRVHRSFIVRLDKIDSIEDNFIHINQKNIPIGKNYK
                     DELMRSLNML"
     gene            complement(4333..5973)
                     /locus_tag="PK610_01910"
     CDS             complement(4333..5973)
                     /locus_tag="PK610_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ34706.1"
                     /translation="MSLWPIISLIISLAAAGTSYLFYRKLLLARKERDEQKAAAQQMQ
                     ESMLEQERYLAYTSHELRTPLNAISGGTQLLSKSALDERQRRYLTTIQSAVDSSLLIV
                     NDILDLAKLDSNMVEVRPVDFLLTEVINNIRHILEFRAEQKGLQFITAVDEHLPPIIH
                     GDSKHLTQILLNLATNAVKFTDLGKVVIRAKEMNREGKTVKIRFEVEDTGKGMRKSSV
                     TTIFNQYQQETRHTIKHAGGTGLGLAITKKLVDLQEGEISVESKYLEGTTFSVELSYG
                     IAEGAIITHKEQDKNLFQQLNHFSILAVDDNMINREILYDLLKDANPYIDIDLAEDGE
                     VAIQKLRSKNYDVVLMDLQMPRKNGYETSEFIRKQFKYPQSEIPIIAMTAHALENVAE
                     DCFRAGMNDYISKPIDMNLLGHKLVKVADKKTRLSEKFSRFKTIDLEHLQELTKDNKD
                     RILKYITIFLSNLPDELEILKQHAEIKDYQSIRESLHKIKGIVAYMGIKSLTRLFASS
                     EYTKLESLSDQELKDFFRQIETTCYQAIEELKIVQKEL"
     gene            complement(5996..7300)
                     /gene="murA"
                     /locus_tag="PK610_01915"
     CDS             complement(5996..7300)
                     /gene="murA"
                     /locus_tag="PK610_01915"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRJ34707.1"
                     /translation="MGTFEIRGGKKLKGELIPQGAKNEALQIISAVLLTPEKVIIRNV
                     PDIIDVNKLISLLQAMGVKVEKLEEGTFSFEAKDVDINYLNSEDFKNRGGGLRGSIMI
                     VGPLLARFGKGYIPKPGGDKIGRRRLDTHFIGFQKLGAKFEYDSKDSFYRVEASNLQG
                     TYMLLDEASVTGTANIIMAAVLAKGRTTIYNAACEPYIQQLCKMLNRMGAKISGVGSN
                     LLYIDGVEYLHGCEHKLLPDMIEIGSFIGLAAMTQSEITIKNVAYPELGMIPEVFGRL
                     GIKMELRGDDLFIPSQECYEVDSFIDGSILTIADAPWPGFTPDLLSIILVVATQAKGS
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGLNRQFPLRGVTMTSPDIRAGV
                     SLLIAALSAEGKSVIHNIEQIDRGYQNIDVRLNKLGAEIKRL"
     gene            complement(7302..7949)
                     /locus_tag="PK610_01920"
     CDS             complement(7302..7949)
                     /locus_tag="PK610_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HRJ34708.1"
                     /translation="MSIETGLNYNTQRPLLHIPEYGRHVQQMVDYCLTIAEREERNKV
                     ANSIITVMGALAPQLRDMEDFKQKLWTHLFVISNFQLDVDSPYPKPTPEAYKERPNRV
                     EYPKKNIRYGHYGKTIESLVKKGVEMEEGEEKTAFILAIANLMKRSYLTWNRDTVADE
                     VIFDQLSNLSAGKIQLKDIELSAKTGTGNSQNEQRSGQHKKKHWNKNFKKNKKRY"
     gene            complement(8094..10325)
                     /locus_tag="PK610_01925"
     CDS             complement(8094..10325)
                     /locus_tag="PK610_01925"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRJ34709.1"
                     /translation="MEFLQDNLIYLIPVMGIVGLIVMAVKSAWVSKQDAGDGTMQELA
                     GYIANGAMAFLRAEWKVLGYFVVIATLLLAYSGTLVEHSSPVIAIAFVIGAVLSALAG
                     YIGMRIATRSNVRTTQAARTSLSKALKVSFTGGSVMGLGVAGLAVLGLGGLFIVFIMM
                     FVPEGSVVTGHEMRKAIEVLTGFSLGAESIALFARVGGGIYTKAADVGADLVGKVEAG
                     IPEDDVRNPATIADNVGDNVGDVAGMGADLFGSYVATILATMVLGQEITSVDNFGGMA
                     PILLPMVIAGVGLLFSIVGTWFVRIKNEHDSVQAALNMGNWSSIIFTAAASYFLVMWM
                     LPETMSWSRGIDITRNGVFMAIIVGLVVGTLMSIITEYYTAMGKRPVNSIIQKSATGH
                     ATNIIGGLSVGMESTVLPIIVLAAGIMTSYYFAGLYGVAIAAAGMMATTAMQLAIDAF
                     GPIADNAGGIAEMSQLPPEVRERTDNLDAVGNTTAAAGKGFAIASAALTSLALFAAFV
                     GIAGISSIDIYKADVLAGLFVGAMIPFIFSSLAIAAVGRAAMDMVNEVRRQFREIPGI
                     MEYKAKPEYEKCVEISTKASIREMILPGAIALIVPVIVGFVFKAEVLGGLLAGVTVSG
                     VLMGIFQNNAGGAWDNAKKSFEKGVVINGETYYKKSEPHKASVTGDTVGDPFKDTSGP
                     SMNILIKLMSIVSLIIAPHIAENQGANTHSDTHQGVSEKAAITTNELPANTISWEAAT
                     TGN"
     gene            10547..>10919
                     /locus_tag="PK610_01930"
     CDS             10547..>10919
                     /locus_tag="PK610_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34710.1"
                     /translation="MTKERSEVDIIRELRTRNALIRKRADGIVLLHHPPEFFSSTFED
                     FLENYKALLSIQQGEISPLLMNVGNVRKVDSASKIFMNKTLPEIAHKMAMVPGNGSAL
                     TRLTVKTYFILHRPPITSKVFR"
BASE COUNT         2778 a         2466 c         2436 g         3239 t
ORIGIN      
        1 catgatgatg aagtaaggtt tccttacccg ggaaaggtat tcccggccga tgatgagtcc
       61 gatcttaccc atggttctga ttttgcgatt gcactacttc tataaaaata tcattcatac
      121 tcggaatgat ttcgcgtaca ctgcggatct ctacatgcgg tatgagtgcg gaaagtaaat
      181 tgttggtggt gttgttgcca agcagtttta ctctcgaacg gaaatgattg ggttctacct
      241 gttctctctc cagtaattca aatcctgccc acaaggcgtt ggcaaaggca atcatgtttc
      301 cgttgaattc cacttcatat acctgcgaac ggaatcgctt gcggatgtcg ctcatctttc
      361 cgtcgagtat cactttcgaa ttgttgatca gcgcaatctc atcacacatc tcttctaccg
      421 atcccatatt gtgggtagag aaaataatgg tggttccgtt ttccttcatg cgaaggatct
      481 cttttttgat cagatcggca ttgatggggt caaatccgct gaatggttca tcgaggatga
      541 gcaatttggg ctggtgcatc accgtagtga taaactgcac tttttgcgcc atccctttcg
      601 agagatcttc aattttttta ttccaccatc cgctgatctc aaatttttca aaccagatct
      661 gtagctgacg tgtggcttcc tgcttgctca ttcccttaag gcgggcaagg tacagggatt
      721 gctctcccac tttcatcttc ttgtacagac cgcgttcttc gggtaagtaa ccaatgttgg
      781 aaagatggtg tggcgaaagt ttttcgtccc ggaaatacac ggtgccttca tcaggaccgg
      841 tgatctgatt gatgatgcgg atgagcgatg tttttcctgc tccgttcgga ccaagtaatc
      901 cgaagatgct tccttcaggt acagcaatgt tcacaccgct cagtgcagtg tggttgcggt
      961 atcgctttac aatattttct gcgcgtaaaa tatccatcag tctccgtggc cggtaaacat
     1021 ttctttccag cgcttaaata tactcttttc ctttttagag ggagaaggag aaaagttttc
     1081 acttccgcgg aattgctcca atgttgcttt ttcttccttg cttaacttct gcggtgtcca
     1141 gatattgatg gttaccaaca ggtcgccttt ctgatagctg tcgagcaccg gtagtccttt
     1201 gttgcgcaac cgaagtattt ttccgctttg tgttccggga tcgatatgga tttttgcctt
     1261 gccttcaagc gtcggaattt cacagtcggc tcccaatgct gcatctgcaa aattgaggtg
     1321 gagatcgtaa tgcaaattat gaccatcacg ctgtaaatga tcgtgtggta attcttcaaa
     1381 taccacaatc atatcaccgg gaataccgtt tctcaccgcg tttcctttgc cgtttactgc
     1441 catctgcata ccttcttcta cacctgctgg aatgttgatg ctgatgactt cttcttcgcg
     1501 tgttgttcct tctccattac agccaccaca tttttcggta acaaccgttc cttgtccgtg
     1561 acaggtaggg caaacctgtg tggtttgcat ttgtccgagg aaggtggaag taacccgtgt
     1621 aacatgaccg gttcctccgc aggtggaaca attgcgaacg gcattcgaat tttttgctcc
     1681 ggttccgtta cagttgcggc aattattcag tttgttgact tttatttttt tctcaacgcc
     1741 ttttgcaatt tcttccagcg taagttttac tttgatgcga agattacttc ctttttgaac
     1801 gcgtctgcct cttccgcctc cgccactgaa tccaccgcca aacgcaccac cgaaaatatc
     1861 gctgaactgg gagaaaatat catccatgtt cattccgcca ccaccatagc cacccgctcc
     1921 tgcgcccatg ccggcatgac caaactggtc gtagcgacga cgtttttccg gatcgcccaa
     1981 tgcatcgtag gcctcggcag cttccctgaa tttttcctcc gcttccttat cgccggggtt
     2041 tttgtcggga tggaatttca gcgccaattt gcggtacgct tttttaattt cgtccgcttc
     2101 ggcgtttctg cttacaccca gtatttcgta ataatcgcgt ttcgacattt tctaaacttt
     2161 tattgtccta ctaccacttt cgcaaaacgt atcaccgtat cgttcatggt gtacccgttt
     2221 tctactacat ccatcacttt tcctttcatc tcatcattgg gtgcaggaac attggcaata
     2281 gcttcagaaa tatctgcatt gaattcctga cccaaaatgt cgatggcttt cacgcctttt
     2341 gcctgcatga tgttcatgaa tttgtgatgg atcaatcgga atccttcctt cacaatttcc
     2401 agatcagtgg ctgtttcgtt ggcgcgaatg gctcgttcaa gatcatcgat gacaggtaga
     2461 atgcttttga ttacatccga accgccgctt ttgatcagat ctgctttctc acgtgctgtg
     2521 cgcttgcggt aattgtcgaa ttcggcatag agcaaaatgt attttttgtt catctcctct
     2581 accggatccg tcgtttcctc tgtcttttcc tcaacggtcg gactctcgtt cccattcccg
     2641 ttcccgttct cattcccgtt cccgttctcg ttcccgttct cattctcgtt catcgtctct
     2701 ttgtttgttg tttcttcctt gctcatacac gtaaaattga ttggactcta tccgtcacaa
     2761 aaaaaagacc aatcccctaa gggcggacaa tgtgtcagaa aggcggtcaa aagtaaggta
     2821 aaatgtactg tcagaagctt tttacgtagg catcaatggc cttgtgcagc tcaagtcccc
     2881 tgaggttggt ggccacgatc ttcccggagg gatcaataag aaagttcatc ggaatcgatt
     2941 tgattccata tacggcgtta atgggtgaat tccagccttt aaggtcgctc acatggtatt
     3001 tccagctgag tttgtcattg gcaatggctt tggtccaagc ttctttttgg gtgtcgagcg
     3061 aaacactaaa gatttcgaat ccggaagcgt ctttgaattt ggcttttgag tacttggccc
     3121 aggcttcggt gagattgggg ttttccttgc gacaaggacc acaccaggag gcccagaaat
     3181 cgatcaatac gtatttgcct ttaatggagc tgagtttgag tgtctttcct tttggattgg
     3241 gtaactcaat tgcaggtgca gtgctgccaa cttctaaaat ctgtaatgaa gaagattcaa
     3301 cggatgaagg gcttcctcca aaacggaaaa tgcccagtgt aaaaaacaaa agggccgagg
     3361 cgccaagcaa aatattgatg cggttcattg agcgcacaag gtacgaaaaa gctaatcttc
     3421 cttgttggtt acccagttga aataattgaa aacagctacc agggccaaaa ctataaagag
     3481 aatgatctta aagacaaata aacttcccat aattctatca gacaattacc taatttaact
     3541 caggctgttc cgatttcagc gggcaaactg attgtcccga tttaggcggt gatgaacgat
     3601 gaaatttcaa ggacggatca agcctgcatc aaagcatgtt cagggatcgc atcaattcgt
     3661 ccttgtagtt cttcccaatg ggaatgttct tctggttgat gtggatgaag ttgtcttcaa
     3721 tgctgtcgat cttatccaga cgcacaatga aggaacggtg aacacgcata aaatcggggg
     3781 aggtaagacg cgattcgatg tctttcattg tcgatagaac gataaatttt ccgccggcag
     3841 tatacacgtt cacgtagttg ccaagtgctt cgatgtacag aatattcgat ttctgaacct
     3901 gtaccagtcg agaatcactt ttaatgaaga tggttttcga tgtttcggaa gagaaatttg
     3961 gacgcgaaag atttttctgt gctttttcca ccgctttcag aaagcgtccg tgcgaaatgg
     4021 gtttcaccac aaaatccgtt acgctgtatt cgtaactttc tgcagcatat tcggcgtgcg
     4081 aagtaataag aataacctgc ggcaatactt cgagactgcg gatgagatcc attccgctca
     4141 ttttgggcat gtctacatcc agaaagatca gatcaacttc ttcggtcttt aaaacgttga
     4201 atgcttctat tgcgctagaa catgttttaa ccaattccag atgctcatgc tcgcgcacaa
     4261 gatcttccat gatattccgg gaaagttcgt cgtcgtcgac aattatacag cgcatgatca
     4321 taataagagg gattatagtt ctttttgtac aatttttaat tcttcaatgg cctggtaaca
     4381 ggtggtctca atttgtctga aaaaatcttt cagctcctgg tcgcttaatg actctaattt
     4441 agtgtattcg gaagacgcaa acaagcgggt cagacttttt attcccatat aggcaacaat
     4501 cccttttatt ttatgcaacg attcgcgtat ggattgataa tcttttattt cagcgtgttg
     4561 tttgaggatc tccagttcat ccggcaggtt ggaaaggaag atggtaatgt attttaatat
     4621 tctgtccttg ttgtctttgg tgagttcctg caggtgttca agatcgattg ttttaaagcg
     4681 gctgaatttt tcggataaac gtgttttttt atcggccact tttacgagtt tatgaccgag
     4741 gagattcatg tcgataggtt tggagatata atcattcatc cccgcacgga aacaatcttc
     4801 tgcaacattt tccagcgcat gagcggtcat ggcaatgatc ggaatttcac tttgcgggta
     4861 cttgaattgt ttgcggatga attcagatgt ctcgtaaccg ttttttcgtg gcatttgcag
     4921 atccataagt accacatcgt agtttttgct gcgtaatttc tgaatagcaa cctcaccgtc
     4981 ttcggcaaga tcgatatcta tatacggatt ggcatctttc agcagatcat aaagtatttc
     5041 gcggttgatc atgttatcgt cgactgcgag aatgctgaaa tgattgagtt gctgaaaaag
     5101 atttttatcc tgctccttgt gggtgatgat ggccccttct gctataccat agctgagttc
     5161 aacactgaat gttgttcctt caaggtattt gctttctaca gaaatttctc cttcctgcaa
     5221 atcgaccagt tttttggtga ttgcaagtcc caagccggtt cctcctgcgt gtttgatggt
     5281 gtgacgcgtt tcctgttggt actgattgaa aatggtggtg accgaacttt tgcgcattcc
     5341 ttttccggtg tcctctactt cgaaccggat tttaaccgtt ttcccttcgc gattcatctc
     5401 ttttgcacga atcactactt tgcccaggtc ggtaaatttt accgcattgg tagccagatt
     5461 gagcaggatt tgagtgaggt gtttggaatc accgtgaata atgggaggaa ggtgctcatc
     5521 aactgctgtg atgaattgca atcctttttg ctctgcgcga aattcaagaa tgtggcggat
     5581 gttgttgatg acttcggtaa ggagaaaatc gaccggacga acttctacca tgttgctgtc
     5641 gagcttggcc agatcgagaa tgtcgtttac tatgagcagc gaactatcaa ccgccgattg
     5701 aatggtggtg aggtatcgac gctgccgttc gtccagtgca gatttggaaa gcaactgtgt
     5761 tcccccggaa atagcgttga gcggagtgcg taattcgtga ctggtatagg caaggtaacg
     5821 ctcttgttcg agcatggatt cctgcatttg ttgtgccgcc gctttttgtt cgtcgcgttc
     5881 ttttcgggcg agaagtaatt ttctgtaaaa aagataactt gttcccgcag cagcgagaga
     5941 aataatcagg gagatgattg gccagaggct cattcagcaa taaaaagttg cacgatcaga
     6001 gacgtttgat ctctgcaccg agtttgttaa ggcggacatc aatattctga taaccgcgat
     6061 cgatctgttc gatgttgtga ataacacttt tcccttcggc cgacaatgcg gcgatgagca
     6121 gggaaactcc tgcacgaata tcgggtgatg tcatcgttac accgcgtaat gggaactgac
     6181 gattcaatcc gattacagtt gcgcggtgcg gatcgcaaag tatgatctgc gctcccatat
     6241 cgatgagttt gtcgacaaag aacaaacggc tttcaaacat tttctggtga atgagaacac
     6301 ttccttttgc ctgcgtggca acaacgagaa tgatactcaa aagatcagga gtaaatccgg
     6361 gccagggcgc atctgcaatg gttaggatgg atccgtcaat gaacgaatcg acttcatagc
     6421 attcctgcga aggaatgaaa agatcgtcgc cgcgcaattc catttttata cccagtcttc
     6481 cgaatacttc aggaatcatt cccaattcag gataggctac attcttaatt gtgatttccg
     6541 attgtgtcat tgctgccaga ccaatgaatg atccgatttc aatcatatcg ggtaacaact
     6601 tgtgttcgca accgtggagg tattcaacac cgtcgatgta caataaattg gaacctacac
     6661 ccgaaatttt tgcgcccatg cgattgagca ttttgcagag ctgttgaatg tagggttcgc
     6721 aggcagcatt gtaaatagta gttcttcctt ttgcaagaac agcggccatg atgatgttgg
     6781 cggttcctgt aaccgatgct tcatcaagca acatgtaagt gccctgaaga ttggatgctt
     6841 caacacggta aaaactatct ttcgaatcgt actcgaattt tgctccgagt ttttggaaac
     6901 cgataaaatg ggtatcaagt ctgcgacgac caatcttatc tcctccgggt ttgggaatgt
     6961 atccttttcc gaaacgcgcg agcaaaggac caacaatcat gatcgatccg cgcaatccgc
     7021 cgcctctgtt tttaaaatct tcggaattca ggtagttgat atcgacatct ttcgcttcaa
     7081 atgagaaagt gccttcttcc agtttttcca ccttaacacc catcgcctgc aagagcgaaa
     7141 tgagtttgtt gacgtcgata atatccggga catttcggat gatcactttt tccggagtaa
     7201 gcaaaaccgc gctgatgatt tgcagtgctt cgttttttgc tccctgagga attaattctc
     7261 cctttagttt ttttcctccg cggatctcaa aagttcccat atcaatatct tttcttgttc
     7321 ttcttgaaat tcttgttcca gtgtttcttt ttgtgctgac cggaacgttg ttcgttctgg
     7381 ctgtttcctg ttcctgtttt tgcagaaagt tcgatatcct tcaactgaat ttttcctgca
     7441 gataaattgc tcaactgatc gaaaataact tcgtcggcaa ctgtatcacg gttccatgtc
     7501 agatacgaac gcttcattaa attggcaatg gccagaatga aagctgtttt ttcttcgccc
     7561 tcttccatct caacaccttt tttaacgagc gattcgatgg ttttaccata gtgaccgtaa
     7621 cgaatattct ttttgggata ttcaacacgg ttgggacgtt ccttatacgc ttcgggtgta
     7681 ggttttgggt agggagaatc cacatccaac tgaaaattgg agatcacaaa aagatgcgtc
     7741 cacaattttt gtttgaagtc ttccatgtcg cgcaactgtg gagccaatgc ccccataacc
     7801 gtgatgatgg agttagcaac cttgttacgc tcttcgcgct cggcaatggt caaacagtaa
     7861 tcaaccattt gctgtacgtg acgaccatat tccggaatgt gcagcaacgg acgctgcgtg
     7921 ttgtaattga gtcctgtttc gatactcata ataaataaac cttggctcta aagatagtta
     7981 atttcaattt gttggtgaag gtgaatagca gtcggcctgt gataaaagct cagccggaca
     8041 actcccaaac aaaaaagccc gctctaaggc gggctttttc tcaatgaaaa caattagttt
     8101 ccggtagttg cagcttccca gctgatggtg ttggcaggaa gttcattcgt tgtgatggcc
     8161 gctttttcag aaacaccctg gtgggtatcg ctgtgcgtgt ttgcaccttg attttccgca
     8221 atatgtggag cgatgatcag ggaaacgatc gacatcaatt tgatcaggat gttcatggaa
     8281 ggtccggagg tgtctttgaa aggatctcca acggtgtctc cggtaactga agccttgtgt
     8341 ggttctgatt tcttgtaata ggtttcgccg ttaatgacaa cacctttttc aaaggatttc
     8401 ttagcattat cccatgcacc gccggcgttg ttctggaaga tacccattaa cacacccgaa
     8461 acagttactc ctgcaagtaa accacccagt acttctgcct tgaatacaaa tccaacaata
     8521 acaggaacaa taagtgcaat ggctcccgga agaatcattt cgcggataga ggccttggtt
     8581 gagatctcaa cacatttttc gtattcgggt ttggctttgt attccatgat acccggaatt
     8641 tcgcggaact ggcgacgaac ttcatttacc atgtccatcg cggcacgtcc taccgctgca
     8701 atagcgagtg atgagaaaat gaaggggatc attgctccaa caaagagtcc ggcaagtaca
     8761 tccgctttgt aaatatcgat ggatgaaatt cctgcaatcc ctacaaacgc tgcgaacaag
     8821 gcaagtgaag taagtgctgc agatgcaatg gcaaatcctt ttccggctgc tgcagtagta
     8881 ttacctactg catccagatt gtcggtgcgc tcacgtactt cgggagggag ctgactcatt
     8941 tctgcaatac caccggcatt gtcggcaatc ggaccaaaag catcaatggc aagctgcatg
     9001 gctgtagttg ccatcattcc tgctgcggca atggcaacac cgtaaagtcc tgcgaaataa
     9061 taggaggtca tgatacctgc agcaagcacg atgattggaa gaactgtaga ttccatacca
     9121 accgataatc ctccaataat gttggtggcg tgaccggttg ctgatttctg aatgatggaa
     9181 ttaacaggac gttttcccat tgctgtatag tactcggtga tgatcgacat cagtgttcca
     9241 actacgagtc caacaatgat agccataaac acaccgttac gggtaatatc aattccacgg
     9301 ctccagctca tggtttcagg aagcatccac attacaagga aataagatgc agctgcagta
     9361 aaaatgatgg aagaccagtt acccatatta agagcagcct gtacagaatc gtgttcattt
     9421 ttgatacgaa caaaccaggt tcctacaata gagaaaagca aacctacacc ggcaataacc
     9481 attgggagca ggataggagc cattccgccg aaattatcta cagaagtgat ttcttgtcct
     9541 aacaccattg tagcaagaat agttgccaca tacgaaccga aaaggtcggc acccatacct
     9601 gcaacgtcac ctacgttatc accaacgtta tcggcaatgg tagcggggtt gcgcacatca
     9661 tcttcaggaa ttccagcttc aactttacct acgaggtcag ctccaacatc ggctgctttg
     9721 gtatagatcc ccccgcctac acgggcaaaa agggcaatgg attcggcacc gagagagaat
     9781 ccggtaagga cttcaatggc tttacgcatt tcgtgacctg taacaactga tccttccgga
     9841 acgaacatca tgataaagac gataaacaaa ccgcccaatc cgagtacggc caatccggca
     9901 actcccaagc ccattaccga accaccggta aacgaaactt taagcgcttt ggaaagactg
     9961 gtacgtgcag cctgggtggt gcgaacgttt gagcgtgttg cgatgcgcat accgatgtaa
    10021 cccgccaatg cagataaaac cgcgccgata acaaaggcaa tggcgattac aggactggaa
    10081 tgttcaacca gtgttcctga ataggccagt aataatgttg cgattacaac aaaataacca
    10141 agcactttcc attctgctct gaggaaagcc attgcaccat tggcaatgta tcctgcaagt
    10201 tcctgcatgg ttccatctcc ggcatcctgc ttggataccc aggcactttt aacggccatt
    10261 acgattaatc ctactatccc cattaccggg ataaggtaga ttagattgtc ttgtaaaaac
    10321 tccataacta aatgattgtg ttaactctta gggtttttga ggggggcaaa agtagggatt
    10381 gataatcaat tctgcaataa agccggggtg ctaaaatgaa acaggggctt ggtcattgat
    10441 ttgcccgtgt ttatccttaa aataggatgc acgcataata aatgtaaccg ggcttcttga
    10501 tttaaggtta aatgggttta gataaggttg cctaacatca taggttatga caaaagaacg
    10561 atcagaagtt gatattatcc gggaattgag gacccgtaat gcattgattc ggaaaagggc
    10621 agatggcatt gtactgctgc atcacccgcc ggaatttttt tcatctacgt tcgaggattt
    10681 tctcgaaaat tataaagcat tgcttagcat tcagcaagga gaaatcagtc ccttgctaat
    10741 gaatgtgggc aatgtgcgaa aggtggatag cgcatcaaaa atctttatga ataagacact
    10801 gcctgaaatt gcccataaaa tggcaatggt gcccggaaat ggaagtgctt tgacgcgttt
    10861 aacagtaaag acgtatttta ttctccacag gcctcccatt acatctaagg tttttcggg
//
LOCUS       DAOMYG010000032        10821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2028_length_10821_cov_7.801783, whole genome shotgun sequence.
ACCESSION   DAOMYG010000032 DAOMYG010000000
VERSION     DAOMYG010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10821
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2028_length_10821_cov_7.801783"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..2370
                     /locus_tag="PK610_01935"
     CDS             106..2370
                     /locus_tag="PK610_01935"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRJ34711.1"
                     /translation="MKRLALIVLTCVLVLTAFLGWRMSKVGFDYDFEKFFPANDPDTE
                     FFLEYRKKFGSDNDFVLFALSNNNGVFREDFLRRVDSFSNELRKIPYVTRVYSIIDYR
                     DLVMTSFSRVPFPVPAIHMDDPESYGPDSSKIFEDNLLVRNLISADAKTVLVYVDTKQ
                     YISKNASDSLALFIDEVMDRYSFDGKISAGRSTGQSYYVKLMQYELGFFAITSFILVI
                     AFLYLSFRTFWAVWVPVTVVVFTAVWIIGFMEIVGEGISLVLTILPTIMFVVGMSDVV
                     HVISRYVEELRSGKGKIDALRITYKEVALATLLTSVTTAVGFLSLLTSTIEPIRDFGL
                     YTALGVMFAFVLAYTLLPAILLLHPVPSIAAIPQEKNFWQRFLSKAFVVVIRKRMLII
                     FISIATAGVSIWGITQMRINNFLLEDLKDGNELKEQFRFFEREFAGVRPLEMAILVKN
                     ADSIPVSRSVLIELEKLENYLITEYGAGTIISPLTLVRSVNRTINGGDKRHYSIPDSD
                     EDLKKVFRMIEKLGADQLLSGMIADNGRTLRLAAKTNDLGSYVYKKKDAELRQFYKRS
                     IDTSVFDYRLTGTALLVDKNNGQVAFGLVWGLGISFVVIAMIVGLMYRSGQMIWIALL
                     PNILPLLMIAGVMGILGIDLKVSTSMMFTISFGIAVDDTIHFISKLRIELSKGRSMMF
                     AVRKTFVSTGRAIILTTIILSGGFLTLIFSDFLGTYYLGLLMALTLFFAVLCDLLLLP
                     VLVIMFYKPRKSEW"
     gene            complement(2573..3409)
                     /locus_tag="PK610_01940"
     CDS             complement(2573..3409)
                     /locus_tag="PK610_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34712.1"
                     /translation="MKKVWNEISKVKYNNRRKADFSYFQPVKAGQYMKSFLQLSLLTA
                     FFLSGQLAFAQFNLKKQLPEKQKFDAANVIDSTYGIMMYERLNPHSEGDSIRLCGSYA
                     CQSWVEDFYTTGTLLHKGFYIDGQLKTYKNYFPNGNVERDFKAIDNYKATCKIYYSNG
                     QLKSDIKYNNGTIESWTDYSEAGVVIYQEVSTKGGDFLEFKKFFFDDGSPKQIMVLAD
                     KKKRSYSYTEYHSNGKVKAQGNKLFSEGLGDYVNDGEWKFFDESGKQSKTETYSNGEK
                     IK"
     gene            3416..4501
                     /locus_tag="PK610_01945"
     CDS             3416..4501
                     /locus_tag="PK610_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HRJ34713.1"
                     /translation="MFQVSKDIREAFTLPGAFYNSEEGLELCRKRLFHRSWQYIADSE
                     VIRLPDSAYPFDFMEGFIEEPLLFTRDKQDNLHCFSNVCTHRGNILIDHPCTLNSGIT
                     CSYHGRRFNIDGTFKSMPECEGMKDFPTPEDDLSKLQVKQWKQFMFTSIDPAFPFEAV
                     FAEMEARVGWMPVDKFVFDPTRSQEYLVRANWALYCDNYLEGFHIPYIHKDLARSLDY
                     QGYASEIYPWSNLQLGIAKGGEMAFDLPKDSPDYGKRVAAYYYWLFPNIMFNFYPWGL
                     SLNIIRPLKHNLTRVIFRSYVWDPSKLDQGAGALLDRVEREDEAIVEKVQKGTNSLLY
                     NKGRYSPKMEQGVHHFHRLITEMLSEK"
     gene            4545..5297
                     /locus_tag="PK610_01950"
     CDS             4545..5297
                     /locus_tag="PK610_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ34714.1"
                     /translation="MKKNAWILFLLWSFGAQAQIQTLTYSDTIVGPGQIIYDVNSDGT
                     DDFQFEIITLSPGGLAQRIVPLNFSAILDNSTFGYPDALLFDDTVASYFNINPGVLGT
                     FVSSGQFNGMGNRYLGMRINATSDAFYGWFYINCSYNRDTIIIRTAAYNSTPWEGINA
                     GQTVITGSDEIAASGMQFSLFPNPANDQLYISSSSFAFPLAYKILDSRGSVVSSAVTN
                     GIVQLSDLPNGIYHLQLEGKDQSAAKTFLISR"
     gene            complement(5298..7016)
                     /locus_tag="PK610_01955"
     CDS             complement(5298..7016)
                     /locus_tag="PK610_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRJ34715.1"
                     /translation="MKRFPLLFLFTLYLTMNLEAQYHSVFEQNKTLTYPEVEQAYRNL
                     EQRYPNCKIYEGGMTDAGRPLLLFTIGKTTDLHREIADPKKVRILINNGIHPGEPDGI
                     DACIYLAQKWLDKKSLPDNVLIGIIPAYNIDGMLNRTCCSRANQNGPEEYGFRGNSQN
                     LDLNRDFIKCDSKNAQSFTEIFHFFDPHLLIDTHVSNGADYQYTMTLLMTQPDKLGPQ
                     GEYCRKVITPRLYEEMKKKGFPMTPYVNTKGETPESGIVDFLETPRFATGYAALFGCI
                     GITSETHMLKAYPQRVKSTIALIETIVSTATIYSKQLEEFRKEMRMTSIVKNETHFHP
                     LNFEPDTNRFSEFEFLGYEANHEPSAISGMMRLKYDRTKPYKKKIRYYDRFTPALMIK
                     VPQAYVIPQAWSRVIDRLQWNKIKLKKIEKDTQFTVTSYTIRSFETVRNPYEGHYLHY
                     KTQTDSKQEIMHFHKGDFIVGTKQAGWRYIVEALEPASVDSYFNWNFFDAVLQQKEWF
                     SDYVFEDLAAELLRKDADLNSRFKQRKKDDPSFAKDGFAQLLFIYRNSPYYEESHNRY
                     PVYRIE"
     gene            7081..7377
                     /locus_tag="PK610_01960"
     CDS             7081..7377
                     /locus_tag="PK610_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HRJ34716.1"
                     /translation="MSQQQEESFIIKYKKELGIGLIAILMLLFIVWNAKPVEFSLVFF
                     TIQISKIFLIALFFVLGMITVWIRFRASNKEKDKRIKELEEKLKKAESGSTTVA"
     gene            7379..8146
                     /locus_tag="PK610_01965"
     CDS             7379..8146
                     /locus_tag="PK610_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006990450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ34717.1"
                     /translation="MRVTFLGTGTSQGIPVIACDCDVCQSTDTRDCRLRTSIMVEHAG
                     RVIVVDTGPDFRQQMLRHHVKRLDAVLFTHEHKDHVAGLDDVRAFNFRQKEKIEIYGT
                     LAVHDALRREFHYAFSDFKYPGVPELSLNEIKNEPFQLLGLPFDPVEVMHYRMPVLGF
                     RIHDFTYITDAKTIAPNEMNKMRGTHTLVINALRKEDHISHLTLSEALEVIAEINPVR
                     AYLIHISHLLGKHAEVEEELPQNVQLAYDGLVLDFQI"
     gene            8255..9448
                     /locus_tag="PK610_01970"
     CDS             8255..9448
                     /locus_tag="PK610_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34718.1"
                     /translation="MNANRRGFTFYRFSIFLFLTFFSFIKTSHAQEVKDSISLLKHDE
                     FYLDRSDRLYLGVSYAQYNYTTIFRHISANLIGDLYTDAGDDNFPKNITRWRFTPSVQ
                     RQFASVSLSSRYGSFSHSRSVDFDAVSQTQMATYNFSIPYRNWSFTLDYLKYTGMNRT
                     DPVYDSTKFLEDMVVRPVTAVFEYTGKGTKHIRAFSRLRGNLLNTPRKMTAALSTLFG
                     YSSMLVQSNSDFIPALQYASSANDPNAILMVNDKYLNKFTSTSVLLGFRCSYYLPLLK
                     YEGRKEFRTLYLKTWIMFLYNVQVYKFYSLTDDFKNTQFKSHQKGNFPTNNLYGFASL
                     VWDAHPWFVSAGANFYSLLYGSGSGACYNGNSVRDQRYSFHLTAAYRIPFAGAIRKVN
                     KVLKR"
     gene            complement(9457..10221)
                     /locus_tag="PK610_01975"
     CDS             complement(9457..10221)
                     /locus_tag="PK610_01975"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRJ34719.1"
                     /translation="MEDLIVYIALTASVLIGGAAVAFMNPTGERSLKLMLAFSGAFLL
                     GVSFLHLIPEVYEHGPANIGVYVLIGFVFQLLLEFFSEGIEHGHVHIHPEAGVKGKFP
                     IVIMLSLCIHAFVEGIPLEREIHSHYHDAANSFHGHHHGDHSLLMGVVFHNIPVSIAL
                     MTMFLKSGLSNTKAFLYLGIFAIMGPLGTFTGHHFGESISGNLEHFFEIVLAVVVGMF
                     MHISTTILFESSENHRFNLYKFIAIISGVLVAMVNF"
     gene            complement(10225..>10821)
                     /locus_tag="PK610_01980"
     CDS             complement(10225..>10821)
                     /locus_tag="PK610_01980"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ34720.1"
                     /translation="RILDLPCGRGRHSVFLEKEGYNVTGADLSSNSIAFAKQFETSEL
                     HFRVHNMLEPFPEKDFDAVMNLFTSLGYFDRPEEDQKALQHMADALKSDGFLIVDFLN
                     PLQVEKNLIPEETRKIDGIIFELNKKIDNKNVIKTVRFRNENKDFEYSEKVHLYSRDE
                     LCSMVNATGLQIINTFGDYHLGPHTEESERCIVVARKS"
BASE COUNT         2912 a         2162 c         2350 g         3397 t
ORIGIN      
        1 gaagtttttc atgggtacaa agtaaacaat cccgttaccg aattgtttcg ttaaaaatac
       61 aaaacgcttt ccacttccct aaaaagccgg tgtatatttg aatccatgaa gcgactggcc
      121 ctaatagtgc ttacctgtgt tttggtttta acggcatttc tgggctggag aatgtcgaaa
      181 gtgggattcg attacgattt cgagaaattt ttcccggcca acgatcccga tacagagttt
      241 ttccttgaat accgaaagaa atttggttcc gacaatgatt ttgtgctgtt tgcactgagc
      301 aacaataatg gtgtttttcg ggaggacttc cttcgtcggg tcgattcatt ttccaacgag
      361 cttcgtaaga ttccttatgt caccagggta tattcaatta tcgattatcg cgatctggta
      421 atgacctcct tcagcagggt tccttttcct gttcctgcta ttcatatgga tgatccggaa
      481 agttatggac ctgattcttc aaaaattttc gaggataatc tcctggtgcg aaacctgatt
      541 tctgccgatg ctaaaacagt attggtgtat gtagatacaa aacaatatat ctccaaaaat
      601 gcttccgatt cactcgcctt gtttattgat gaggtaatgg atcgttactc cttcgatgga
      661 aagatctctg ccggaagaag caccggtcaa agttactacg taaaactcat gcaatatgag
      721 cttggatttt ttgccattac ttcttttata cttgtaattg cgtttttgta tttgtctttt
      781 cgtacgtttt gggcggtttg ggttcctgta accgtagttg tttttacagc tgtgtggatc
      841 ataggtttca tggagattgt tggagaagga atcagtttag tccttaccat tcttccaacg
      901 ataatgtttg ttgtgggaat gtcggatgtg gtgcatgtta tttcccgcta cgttgaagaa
      961 ttacggtccg gaaaaggaaa aattgatgcg ctgcgaatta cctacaaaga ggtggcattg
     1021 gcaacattgc ttacttctgt taccacggct gtcggatttt tatctttact cacttccacg
     1081 attgaaccga ttcgcgattt tggattgtat actgcattag gggtaatgtt tgcatttgtt
     1141 ctcgcatata ccctgttacc cgctattttg cttttacatc ctgtaccatc cattgctgca
     1201 attccgcagg aaaagaattt ctggcaacga ttcttaagta aagcctttgt tgttgtgata
     1261 cgcaaaagga tgttgattat ttttatcagc attgctactg caggagtttc catttgggga
     1321 attacgcaga tgcgaatcaa taattttctt ctcgaggatc ttaaagacgg caatgaatta
     1381 aaagaacagt tccggttttt tgaacgtgaa tttgcaggtg tgcgtccgct cgaaatggct
     1441 attctggtaa agaatgcaga ttccattcct gtttcccgca gcgtgctaat cgagttggaa
     1501 aaattggaaa actaccttat taccgaatac ggagccggta ctattatttc tccgctcaca
     1561 ctggtgaggt cggtaaacag aacaataaac ggtggcgata aaaggcatta ttccattcct
     1621 gattcggatg aagatctgaa aaaggtattc cggatgatcg aaaaacttgg ggcagatcag
     1681 ttgctcagtg gaatgattgc tgataatgga agaacgcttc gtttggccgc aaaaaccaat
     1741 gatctgggaa gttatgtgta caagaaaaag gatgctgaat taagacagtt ttacaagcga
     1801 tccatcgata cctccgtgtt tgattaccgg cttaccggaa cggcattgtt ggtggacaag
     1861 aacaatggtc aggttgcatt cggattggtt tggggcttgg gcatttcatt tgtggttatt
     1921 gcaatgatcg ttggattgat gtatcgttca ggacaaatga tatggatcgc attattgccg
     1981 aatattctac ccttgttgat gatcgcaggt gtgatgggaa ttcttggaat agatcttaaa
     2041 gtatccacct ccatgatgtt taccatcagt tttggaattg ccgttgatga taccattcat
     2101 ttcatttcca aattgcgcat cgaactatcg aagggcagat cgatgatgtt tgctgttcga
     2161 aaaacgtttg tgagtacagg ccgtgcaata attcttacca ccattatact ctccggtgga
     2221 tttttaacac ttattttctc cgattttctg ggaacgtatt acctcggctt actaatggcg
     2281 cttacactgt tttttgcagt gctctgtgat ttgctgctat tgccggtatt ggtgatcatg
     2341 ttttacaaac cccgaaagag tgaatggtga atggtgagca gtgaaaagtg aacggtgaaa
     2401 agtgatcccg acgcgattgg tcgggacagg ctggtgaacc gtaactctta actaataact
     2461 cctacctcct aaccaataac tcctaattga taaccgataa ctgataacca ataacatcca
     2521 acatcaaatt gaatagaata gaaaaatcgg taaagatgtt ttcgggtaac gattatttta
     2581 ttttctcgcc attggaatag gtttctgttt tggattgttt tccgctttca tcgaaaaatt
     2641 tccattcacc atcgttaacg taatctccaa gtccttctga gaaaagtttg ttcccctgag
     2701 cctttacttt tccgttggaa tggtattcgg tgtaggaata cgaacgcttc tttttgtcgg
     2761 ccaaaaccat gatttgtttt ggagacccat catcaaaaaa gaatttcttg aattccagaa
     2821 aatcaccgcc tttggtgctt acttcctggt aaatcacaac tcctgcttca ctgtagtcgg
     2881 tccaggattc gattgtacca ttgttgtact tgatgtcgga tttcaattgt ccgttggagt
     2941 aatagatctt acacgtggct ttgtaattat cgatcgcttt aaaatcgcgt tcaacattgc
     3001 cattagggaa atagttttta tacgtcttaa gctgaccatc aatataaaaa cctttatgca
     3061 gaagggttcc ggtggtataa aaatcctcca cccagctctg gcaggcatac gatccgcata
     3121 gacgaatgga gtcgccttca ctatgtggat tcagtcgttc gtacatcatg atgccatagg
     3181 tggaatcaat cacgttggca gcgtcaaact tttgtttttc gggtaactgc ttcttaaggt
     3241 tgaactgtgc aaatgcaagt tgaccggaca gaaaaaaggc agtaagtagg ctgagttgta
     3301 gaaatgactt catgtattgt ccggctttaa caggttgaaa atacgaaaaa tccgctttac
     3361 gacggttatt atactttacc ttgctaattt cgttccaaac ttttttcaac aatttatgtt
     3421 tcaggtatcc aaagatatac gggaagcgtt cactcttcct ggcgcatttt acaacagcga
     3481 agaaggcctc gaattgtgcc gcaagcgttt gtttcaccgt tcatggcaat acatcgcaga
     3541 tagtgaggta atccgattgc ccgattctgc ctatccattt gattttatgg aaggttttat
     3601 cgaagaacca cttctgttta ccagagataa acaggataat ttgcactgtt tttccaatgt
     3661 ttgtacccat cgtggtaata tcctgatcga ccatccatgt acccttaatt cgggtataac
     3721 gtgttcgtat catggcagaa ggtttaatat cgacggcact ttcaaaagca tgcctgagtg
     3781 tgaggggatg aaggattttc caactcctga ggatgatctc tcaaaattgc aggtaaaaca
     3841 atggaaacaa ttcatgttca cctcgataga ccccgcgttc ccttttgaag cagtattcgc
     3901 agaaatggag gcccgggtgg ggtggatgcc tgttgataaa tttgtttttg atcctacccg
     3961 ttcacaggag tatctggtac gtgcgaactg ggctttgtac tgcgataatt acctggaggg
     4021 ttttcacatt ccctatatcc acaaagatct tgcccgctcg ctcgattatc agggctatgc
     4081 cagtgagatc tatccctggt cgaacctgca attgggtatt gcaaaaggtg gtgagatggc
     4141 gtttgatctg cccaaggatt cgcccgatta cggtaagcgg gttgctgcgt attactattg
     4201 gttgtttccc aatatcatgt tcaattttta tccatgggga ttatccctga atattattcg
     4261 tccgctcaaa cataacctta cgcgtgtcat cttccgttcc tatgtatggg acccctccaa
     4321 actcgatcag ggggcaggtg cattgctcga tcgtgtggag cgggaagatg aggccatcgt
     4381 ggaaaaggtt caaaagggta cgaattcatt attgtacaat aaaggcaggt attcacccaa
     4441 aatggaacaa ggggtacatc attttcatcg gcttattact gaaatgttga gtgagaaatg
     4501 agaatcatta tatatgtaaa accgatttct tattaatcat tattatgaaa aagaatgctt
     4561 ggatattgtt tttgctttgg agtttcggtg cacaagcaca gattcaaacg ctgacctata
     4621 gtgatactat cgttggaccg ggacaaatta tctatgatgt taattccgac ggtaccgatg
     4681 attttcaatt tgaaattatc acgctttctc cggggggatt ggcgcagcga attgttccct
     4741 taaatttttc tgctatcctc gataattcga cattcggtta tcccgatgcc cttttgtttg
     4801 atgatactgt tgcttcgtat tttaacatca atccgggtgt attgggaacc ttcgtttctt
     4861 ctggtcagtt taacggaatg ggaaatcgct atctgggaat gcgcatcaat gcaactagtg
     4921 atgctttcta tggttggttt tatatcaatt gcagttacaa tcgcgatacc atcatcattc
     4981 gtactgctgc ttacaattca actccatggg aaggaatcaa tgccggacaa acggttatta
     5041 caggttcaga cgaaatcgcc gcttccggga tgcaattttc actttttccg aacccggcaa
     5101 atgatcagct ttacatttct tcctcctcat tcgcatttcc acttgcatac aaaattctgg
     5161 acagccgcgg tagtgtggtt tcttcggctg ttacaaacgg aatcgttcag ctcagcgatt
     5221 tacccaatgg gatttatcat ttgcaactgg aaggcaagga tcaatctgcc gctaaaacat
     5281 ttttgatctc ccgctgatta ttcgatgcgg tagaccggat aacggttatg gctttcttcg
     5341 taatagggag agttgcggta aatgaaaagc agttgtgcaa atccatcttt tgcaaacgaa
     5401 ggatcatctt tttttcgctg tttaaatcgt gaattcaggt ctgcatcttt tcgcagtaat
     5461 tctgccgcga gatcttcaaa tacgtaatcc gaaaaccatt ctttttgttg taaaacagca
     5521 tcaaagaaat tccagttgaa ataagaatca accgaggcag gctcaagtgc ttcaacgata
     5581 tatctccagc ctgcctgttt ggtgccgaca ataaaatcgc ctttgtgaaa atgcatgatt
     5641 tcctgtttgc tgtcggtttg tgttttgtaa tgcagataat gtccttcgta cggattcctt
     5701 acggtttcga agctgcgaat ggtgtagctt gtaacggtaa actgggtgtc cttttcaatt
     5761 tttttcagtt ttattttatt ccattgcaag cggtcaataa cgcgcgacca tgcctgagga
     5821 attacgtaag cttgtggcac ctttatcata agcgcaggag taaaacgatc gtaataacga
     5881 atcttttttt tgtagggttt ggttcgatcg tattttaagc gcatcatccc ggagatggca
     5941 ctgggttcgt gattcgcttc gtaacccaga aattcaaatt cagaaaaacg gttggtgtcc
     6001 ggttcaaaat tcagcggatg aaaatgtgtt tcgtttttca caatggatgt catcctcatt
     6061 tcttttctga attcttcaag ctgcttgctg taaatcgttg cggtggaaac aatggtttca
     6121 attaatgcga tggtgctttt tacccgctgg ggatacgctt tgagcatatg tgtttcgctg
     6181 gtaattccga tgcatccaaa aagtgctgca tatccggttg caaatcgtgg agtttccaaa
     6241 aaatcaacga tcccgctttc gggtgtttct ccttttgtat ttacataagg agtcatcgga
     6301 aatccttttt tcttcatctc ctcgtacagg cgtggtgtaa ttacttttct gcaatattct
     6361 ccctgcggac caagtttgtc gggttgggtc atcaataatg tcatggtata ttgataatct
     6421 gctccattcg aaacatgggt gtcgatgagc aaatggggat cgaaaaagtg aaagatttca
     6481 gtaaaggatt gtgcgttttt ggaatcgcat ttaataaaat cccgattcag atccagattt
     6541 tgtgagttgc ccctgaatcc gtattcctcc ggaccatttt ggttggcccg gctgcagcag
     6601 gttctgttga gcataccgtc aatattgtag gcgggaataa ttccgatcag cacattatca
     6661 ggcagggatt ttttatccag ccatttttgc gccagataaa tgcatgcatc aataccatcc
     6721 ggttctccgg ggtgaattcc attattgatt aggatcctta cttttttggg atcagcaatt
     6781 tcacgatgca gatcagttgt ttttccaata gtgaacagaa gcagtgggcg accggcatct
     6841 gtcattccgc cttcatagat tttacaattc gggtaacgtt gctccagatt gcggtaggcc
     6901 tgctcaacct cgggataggt tagagtctta ttttgctcaa aaaccgaatg gtactgagcc
     6961 tccagattca ttgttaaata cagggtgaaa aggaagagaa ggggaaagcg cttcattttt
     7021 ttttgacttg aattagcgta tacgactcaa aataggtaat ttttcagcaa aatcaggaat
     7081 atgtcacaac aacaagaaga atcgttcatt atcaagtaca aaaaggagct aggcattggt
     7141 cttattgcca tattgatgtt gctctttatc gtttggaatg caaagccggt tgaattctca
     7201 ctggtgtttt ttaccatcca gatttcaaag atcttcctaa tcgcattgtt ttttgtattg
     7261 ggaatgataa ccgtttggat ccgattccgt gcatccaata aagaaaagga caagcgcatt
     7321 aaggagcttg aagaaaaatt gaaaaaagca gaatcgggat ccaccacagt tgcctgatat
     7381 gcgggtcact tttttgggta caggaacttc gcagggtata cctgtaattg catgcgactg
     7441 tgatgtctgc caatcaaccg atacgcgcga ttgcagattg cggacctcta tcatggttga
     7501 acatgccgga agagtaattg tagtggatac aggtcccgat tttcggcagc agatgcttcg
     7561 gcatcatgtt aagcgtttgg atgctgtgct gtttacacat gaacacaaag atcatgtagc
     7621 aggattggat gatgtacgtg catttaattt tcgtcagaag gaaaaaattg aaatctacgg
     7681 aacactggca gttcacgatg cgctgcgacg ggaattccat tatgcgtttt ctgatttcaa
     7741 atatcccggt gttccggaat taagtctcaa tgaaataaaa aacgagccct ttcaactcct
     7801 gggtttacct tttgatccgg ttgaggttat gcattaccga atgcctgttc ttggatttcg
     7861 tatccacgat tttacctata tcaccgatgc gaaaaccatt gctccaaatg aaatgaataa
     7921 aatgaggggg acacatacgc ttgtgatcaa tgctctccga aaagaagatc atatttctca
     7981 tcttacccta agtgaggctt tggaggttat cgcagaaatc aatcctgttc gtgcttacct
     8041 tattcatatc agccacttac tgggtaagca tgccgaggtg gaggaagaat tgcctcagaa
     8101 tgtgcaactg gcctacgatg ggttggtgct ggactttcag atttaggtct gcagtaacct
     8161 gatccttagc tgataatttt ttgaattgtt tagaaggaga atcaaagtga aaatgctgaa
     8221 tagctttggt atattcgcgt gaattttttg aggtatgaac gcaaaccgga ggggatttac
     8281 attttatcgc ttttcgatat ttttattcct gacttttttt tcatttataa aaacttccca
     8341 cgcccaggag gtaaaagatt ctatatcgct cttaaaacat gatgagtttt atctggatcg
     8401 ttccgacagg ctttatctgg gtgtgtcgta tgctcagtac aactacacga ccatcttccg
     8461 gcatatttct gccaatctga ttggtgattt gtatacggat gcgggtgatg ataatttccc
     8521 aaaaaatata acccggtgga gatttacacc ttccgttcaa cgtcagtttg catcggtttc
     8581 actttcctcc cgatacggtt ctttttcgca ttccagatct gtcgattttg atgcagtttc
     8641 gcaaactcaa atggcaacct ataatttttc cattccctat aggaattgga gttttacatt
     8701 ggattatctc aagtatacag gtatgaaccg taccgatccg gtttacgaca gtacgaagtt
     8761 tctggaggat atggtagttc gtcctgttac cgcagttttt gaatacaccg gtaagggaac
     8821 aaaacacatt cgggcattta gtcgtttacg cggaaattta ctgaacacac cacgaaaaat
     8881 gactgcggct ttgtcaacct tattcgggta ttcttcaatg ctggttcaaa gcaattccga
     8941 ttttatcccc gcgcttcagt atgcgagttc ggccaatgat cccaatgcca ttctaatggt
     9001 caacgataaa tacctgaata aattcaccag tacaagtgtg ttgctggggt ttcgttgttc
     9061 gtattatttg ccattgctga aatatgaagg gcgaaaggag ttccgtacgc tatacctgaa
     9121 aacctggatc atgtttttgt ataatgttca ggtgtataaa ttctattcgc tgaccgatga
     9181 tttcaagaat acgcaattta aaagtcacca aaagggaaat ttcccaacta acaatcttta
     9241 cggatttgcc agtttggtat gggatgcgca tccttggttt gttagtgccg gtgctaattt
     9301 ttattccttg ttgtatggtt cgggttcagg agcttgttat aacggcaata gtgttcgcga
     9361 tcaacgttat tcctttcatc tcactgcagc atacagaatt ccatttgcag gggcaattcg
     9421 taaggtaaat aaagtgttga aaaggtaatt ccggatctag aaatttacca tcgcaacaag
     9481 aacaccggaa ataatggcga tgaacttgta cagattgaac cgatgatttt cggaggattc
     9541 gaataatatg gtggttgaaa tatgcataaa cataccaact accacagcca gaacgatctc
     9601 aaaaaagtgc tcaagattac cggaaatgct ttctccgaaa tggtgaccgg tgaaagtacc
     9661 taatggaccc attatcgcaa atattcctaa atagagaaaa gctttagtat tgcttaatcc
     9721 gctcttcaga aacatggtca taagcgcaat ggaaacaggg atgttgtgaa atacgactcc
     9781 cattagcagc gagtgatcgc catggtggtg accgtgaaat gaatttgctg catcatgata
     9841 gtgagagtga atttcccgtt ccaatggaat tccttctacg aatgcatgaa tacataatga
     9901 tagcatgatt acaattggaa attttccttt tactcccgcc tccgggtgaa tatgtacgtg
     9961 tccgtgttcg attccttcgc tgaagaattc aaggagcaac tgaaaaacaa atccgatcag
    10021 aacatacaca ccgatattgg ccggaccatg ttcgtatact tcaggaatca ggtggagaaa
    10081 tgaaacgcca agaaggaagg ctccactgaa tgcaagcatc aattttaacg atcgctcacc
    10141 ggtagggttc ataaatgcaa ctgccgcgcc cccaattaaa accgaggcgg ttagtgcgat
    10201 ataaacgatc agatcttcca caaatcagga ctttcgggca acaacgatgc agcgttcgct
    10261 ttcttcggta tgtggcccca ggtgataatc tccaaaggta ttaattattt gcaatcctgt
    10321 tgcattcacc atgctgcaaa gttcatcacg actgtataaa tgaacctttt ccgaatattc
    10381 aaaatccttg ttttcgttac ggaaacgcac cgtttttatt acatttttat tgtcaatttt
    10441 tttattcagc tcgaaaatta ttccgtcaat tttgcgcgtt tcttcaggaa tcagattttt
    10501 ttcaacctga agaggattca aaaagtctac gatcaaaaaa ccatcggatt tcagcgcatc
    10561 tgccatgtgt tgcaaggctt tctgatcttc ttccgggcga tcgaaatatc ccagactggt
    10621 gaataaattc attaccgcat caaagtcctt ttctggaaat ggttccagca tgttgtgcac
    10681 ccgaaaatga agttcggatg tttcaaattg cttcgcaaaa gcaatactgt tgctggaaag
    10741 atccgcaccg gttacattgt atccttcttt ctcaaggaaa acggaatgac gacctcttcc
    10801 acagggaaga tccaatatgc g
//
LOCUS       DAOMYG010000033        10816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2030_length_10816_cov_11.604498, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000033 DAOMYG010000000
VERSION     DAOMYG010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10816
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2030_length_10816_cov_11.604498"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(331..1578)
                     /locus_tag="PK610_01985"
     CDS             complement(331..1578)
                     /locus_tag="PK610_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34721.1"
                     /translation="MPVDPSMADAMLGTFRTMAKDCQDKGSNGEAFDNMMAALNRMEQ
                     LATELNDVSDLSGALMNENLYMEFSNNYTKVLSAAYKSDYDPNAPYDEKADQALMKQS
                     LNAYRDAIRQIKDSKEETKKLMGDRSSDLDVLYKEQRMVDAIQAVIDLGESGISYAEW
                     CKRLITQGLDKAMEGSVLSRENVEWDVKFAEAMAVSPAHIEAAKRKLQKFDELASQSP
                     IKMPETFKYQLACDKIDWELEPEQLKWYWVKDKWDKLFNRLEEWIMSYTSMAPYIFPW
                     NQHSNPQASVKESQACAPGLFRAWENMCKDYFGMNAEEMFKHPSFDWEIRYGFHMHSK
                     VFTEFLLNEIMPICNPNAKPSDELVKKAEEMHKNHMVYRPDNNIPYHRMKKAFDERFG
                     EGWYVQRHGLPGEGAKSNAEPWN"
     gene            complement(1583..2233)
                     /locus_tag="PK610_01990"
     CDS             complement(1583..2233)
                     /locus_tag="PK610_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34722.1"
                     /translation="MTDYLKEYQEYYRVRMQRYENDPEYTDSYQSEKAIYEAIAGVNE
                     MEGFRERLGNLNELNAVAYTRDKQRMYLRHFESMQETVRAQTPKRIMDKAPSYEKVMD
                     LIEMINNEEAKGMLEITADSVSPFDNWILLEYIELYSKADVPEQYKSTYKKYIDDYTK
                     DQRERLAHSEGEIGKFVPGWKFKKDLPTEFRHRRKFPVNDAAFEARLKQFIQINNL"
     gene            complement(2504..3853)
                     /locus_tag="PK610_01995"
     CDS             complement(2504..3853)
                     /locus_tag="PK610_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ34723.1"
                     /translation="MKLLQVTESGFKNTVRTALTALGIILSVNSYAQVSELIWGDPVK
                     LPATINSPGEEALLVFSRDSSTLFFVRQFFDDNTGGNEDQDIYYSKRGVGGIYDAAKN
                     LKALNNKDFNAVVGVSLDGNTVYLYNAYVKKKNQLDRGIAVAKKRPNGTYSDPVKLEI
                     AGFKLTGNHFGFYVNPQETVILISFEGPGSLGEEDLYVSLRQPDGSWGIPIHLGNAIN
                     SKGFEMSPFLSEDGYSLFFSSNGHGGEGDADIFVCFRMDETWRNWSKPQNLGNKVNSP
                     GFDAYFILSANDAYFASNRDGGTSDIYNVKPLLPEPEPEPVPEPEPVVEEKPEEIARK
                     ELFSMEFKIYFATNSSWLEKQAIDAIDAALEKLKQYPEIKCNISSFADKRADDNYNVW
                     LTQRRAFRVRDYLVLRGVDPSRITCYYHGKKNPVVNCKDCTEEELRLSRRTVITFYE"
     gene            complement(3819..4787)
                     /locus_tag="PK610_02000"
     CDS             complement(3819..4787)
                     /locus_tag="PK610_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRJ34724.1"
                     /translation="MKKLFIASALLVSGGIASAQQMQQLSQYLLNPYVINPAAAGLTD
                     FVDLNLSFRQQWVGFENSPQTFYLSGNSVVGNVGGSPKYSASLRTSRQTVQKNTGIRT
                     GKMRHAVGGNVMGDRYGAFKRNMFNGSYALHLPVANGINASIGVGVGMSNVIFDQAKV
                     TMLDPNDNTYAQFLGNTTRGNLFDVFTGVYLYSEQFQVGYSSAQLLQNKLYFGDPTNA
                     KLKMHHFFMGGYRFDVNEKLSITPNFLLKYMNPAPMAIDLNCKVDYDNMFFGGFSYRH
                     KDAIVGMLGMFWNNIKIGYSYDYTISSLRKQNSGGHEIVAGYRIRI"
     gene            complement(4807..9360)
                     /locus_tag="PK610_02005"
     CDS             complement(4807..9360)
                     /locus_tag="PK610_02005"
                     /inference="COORDINATES: protein motif:HMM:NF014544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HRJ34725.1"
                     /translation="MKKFLLATIGFAFLSSAAIAQTYLHPTTGIQNTYAGNCMVSGCT
                     GNYYDNGGAAGNYSNNINNIYRTFCPNTPGQCMRLTFQNSVALDQDGFGCWDFLIITD
                     SPTQNGPTLFSGCNIPAGTTFTSNNPSGCITVRFLSDNVINTPGWFATLSCVPCSGGA
                     GIGPQSNTIADCNNAQFVCDNLNPVTANSSGPGLIADACVSDCVIAENYSNWYIINVT
                     TPGTFGMTIVPNPVAADYDFALYGPVVGGCSGLGTPIRCSYASNTGNTGMGNGAVDNS
                     EDVNGNGWVAPANLAVGTYYLLINQWDDYPSAGFQLDFNGTAVVGLPSPVPSAAPVSC
                     SNSVLNLSVPTIAGATYSWTGPGGWTSNVQNPSRNPVVAGTYTLTYTVNGCTSPGGSV
                     VVTIPPDVTAPTITCPSNQTANTSAGSCTASVTIPNPVTNDNCQVSTLTWAMTGATVA
                     NSPGAGINNVGTFTFNAGVTTVTYTVRDASSNPATCSFTVTVTDNINPTITCPSNQTG
                     TTGAAGCTASVTSPNPTTGDNCGVTTLTWAMTGATTGNSPGAGINNIGTQTFNTGVTT
                     VTYTVRDAANNVATCSYTVTVTDNAAPTITGCPANTTGNVNASCQYIVPSFTGSITVS
                     DNCTAPASITRTQSPVAGTILGVGVHSITINATDAAGNTANCSFTITVTDNSSPLITG
                     CPANTNGNVNASCQYTVPNFATSITVSDNCTPSGSITVVQSPAAGSNLGVGVHTITLT
                     ATDANGNNANCSFTVTVTDNTVPVISGCPSNQTANVNASCTYTVPNFTTSITVSDNCT
                     PAGSIVVSQSPVAGTTLGVGNHTITITATDAGTNIATCSFQVSVADTQNPVITGCPGN
                     QNVTANASCQFVIADYLSGITATDNCGMFSLTQSPIPGTVVSGTQTVTIQATDNNGNI
                     STCTFQVIVADNTPPTITGGCPANTTGNVNASCQYIVPNFNSSISVTDNCSSGANITI
                     TQSPAAGTALGTGTHTITITVADANGNSDNCSFTVTVTDASAPTFTSCPPNQTVPGNA
                     SCQGVLGDYTSTAIVNDNCGGSASVTVTQSPAAGTTISGPTTVTLTATDASGNANTCT
                     FSVSVADAIAPTFVTCAPNQNEAPDANCQFTLPSYTTLATVSDNCSAAGSITITQSPS
                     AGTVITGTTIVTLTATDGAGNSATCTFSVILTDNTAPVVTNCVSPQNVNITSGCTHTL
                     GDYTSQVTYTDNCGSSSVTVTQNPAAGTAISAGTTTVTLTLTDANGNQSTCNFTVTVA
                     DTEAPVINCTANINSCNQVVTFVAPTATDNCGTATVVQTDATGLSSGSTFPIGTTTLT
                     YSATDAAGNISTCSFTVTVDAATSPAVAGPDVIVCDTVNTVTITATAPSNGTGTWVIL
                     SGAGTIGTPTSATTTVTNLSNGTSVLAWVVTPTGACPPTSDSLLIVMSTCFEIIIPTG
                     FTPDGDGTNDVWEIPFLNLYHPNCTVQVFNRWGAKLFESTGYADPWDGSFKGNKMPVG
                     SYYFVIDFADGSDPVKGTVTIIR"
     gene            complement(9366..9866)
                     /locus_tag="PK610_02010"
     CDS             complement(9366..9866)
                     /locus_tag="PK610_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34726.1"
                     /translation="MKNYKLLLALVISLFSVSVSAQKDANQPERGYPDFYMELIGIDD
                     PTYPRLGNGVTAPNVEVYLEQVRQWCSANPVKFYQYLENRAANPKFTSVQQRELPDAK
                     ALKARSHLRMIEQVILLQRKLNIQFPFVEELAKQKQAVFQVPTKIYLVLIHKDDLDLF
                     FNPEKL"
     gene            complement(10542..10727)
                     /locus_tag="PK610_02015"
     CDS             complement(10542..10727)
                     /locus_tag="PK610_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34727.1"
                     /translation="MKKVIFASLIAVALVACGGETKTDEKAADSTATAAPAPVEEPAP
                     VEAPADTTAPATEEAAH"
BASE COUNT         2868 a         2293 c         2440 g         3215 t
ORIGIN      
        1 tccaacaaaa tggattttac ttcccttctc ccaacttcct tcaaacgtag aagtaggatt
       61 aaaagcagaa gtccaaaact cataagtgct tttatcctta agacccaaca tcgtttcata
      121 aactttctgc gaagatgcct tgatctcttt tttaaactga agttttttca tttttcaaat
      181 cgtttttaat caaactttaa ttattcaact atttccaacc aaacattcct gtactcacac
      241 attctcgcat tctcccattc acacattacc ccattacccc attaccccat tatcccattc
      301 ccccattaat acattcgcac attgtcccat ttaattccac ggctccgcat tcgatttcgc
      361 accctcaccc ggtaaaccat gtcgctgcac ataccaaccc tcaccaaaac gctcgtcgaa
      421 agcttttttc attcgatggt aaggaatgtt attgtcagga cgatacacca tgtgattctt
      481 gtgcatctct tccgcttttt ttacgagttc atctgaaggt tttgcattgg gattgcaaat
      541 cggcattatt tcgttgagca aaaattccgt gaacactttc gaatgcatat ggaacccgta
      601 acggatttcc caatcgaacg aaggatgttt gaacatctct tctgcattca ttccaaaata
      661 atccttgcac atgttttccc atgcacgaaa caatccgggt gcgcaggcct gactttcctt
      721 caccgatgct tgcggattgg aatgctgatt ccagggaaaa atatacggcg ccatggaagt
      781 ataggacatg atccattctt cgagacgatt gaagagttta tcccatttat cttttaccca
      841 ataccatttc agttgttcgg gttccagttc ccagtctatt ttgtcgcaag ccaattggta
      901 cttgaaggtc tccggcattt tgattggcga ttgtgaagcg agttcatcga atttctgaag
      961 tttgcgttta gcagcttcta tatgcgcggg cgaaacagcc attgcttcgg caaacttcac
     1021 atcccattcc acattttctc tgctcagcac cgaaccttcc atcgctttgt cgagtccttg
     1081 tgtaatcaaa cgtttgcacc actcggcata actgattccg gattcaccca gatcaattac
     1141 ggcctgaata gcatctacca tgcgttgttc cttgtaaaga acatcaagat cggaagaacg
     1201 gtcgcccatt agttttttcg tttcttcctt gctgtccttg atctgacgaa tggcatcgcg
     1261 gtaggcattg agcgattgtt tcattaaggc ctgatccgct ttttcatcat agggtgcatt
     1321 cggatcgtaa tcggatttat aggcagcact cagcactttg gtgtaattgt tcgaaaattc
     1381 catatataaa ttttcgttca tcaaggcacc cgataaatca ctgacatcat tcagttctgt
     1441 ggcaagttgt tccattcggt taagcgcagc catcatgtta tcgaaggctt ctccgttgga
     1501 acctttgtcc tgacaatcct tcgccattgt gcgaaaggta ccaagcatgg catctgccat
     1561 tgaaggatct actggcatac gattaaagat tgttgatttg aataaattgt ttcagtcgcg
     1621 cttcaaacgc agcatcattc accgggaatt ttctgcggtg acgaaattct gtaggaagat
     1681 ctttcttgaa cttccatcca ggaacaaact tcccgatttc tccttcgctg tgcgctaaac
     1741 gctcacgttg atctttggtg taatcgtcga tgtatttctt gtaggtggac ttgtattgtt
     1801 caggcacatc tgccttactg tagagttcaa tatattccag gaggatccag ttatcaaaag
     1861 gactaacgga atctgcagta atctccagca ttccttttgc ttcttcgtta ttgatcatct
     1921 caataagatc catcactttt tcgtaggatg gtgctttatc cataatgcgc tttggtgttt
     1981 gcgctcttac ggtttcctgc atcgattcga aatggcggag atacatgcgt tgcttgtcgc
     2041 gggtataggc caccgcattt aattcgttca gattacccag gcgttcacga aagccttcca
     2101 tttcatttac tccggcaatg gcttcgtata tcgctttttc actttggtaa ctgtccgtgt
     2161 attccggatc gttttcatag cgctgcatgc gcacccgata atattcctgg tactctttta
     2221 aataatcagt catggcaatg attttgccac aaattaatca agacttggga tttttcctaa
     2281 ttggaagcgg atagcagtgt acagaaatct cgacgctccc cgacgctccc ggtcggggca
     2341 ggatcggtcg ggacaggtcg atgaacggtg aacagtaatc ggtgaacggt aaacggtgaa
     2401 cagtgaatgg taattggtaa ttgggtaatt ggaaattgga aatcggataa ttggaaattg
     2461 ggtaattgga aattgggtaa ttaataatcg tcttcgttcc cgtttactca taaaacgtaa
     2521 tcaccgtcct tcttgaaagt cgtaactcct cctcagtaca atccttacaa ttcaccacag
     2581 gatttttctt accgtggtag tagcaggtaa ttcgggaagg atcaacacca cgtaatacga
     2641 ggtaatcgcg tacacggaaa gcccttcgct gcgttagcca tacgttataa ttatcgtcgg
     2701 cccgtttatc ggcaaaggaa ctgatgttgc acttgatttc cggatactgt tttaattttt
     2761 caagcgctgc atcgatcgca tctatcgctt gtttttcgag ccaggaagaa ttggtggcga
     2821 aataaatttt aaactccatc gaaaaaagtt ccttacgggc aatttcttcg ggtttttctt
     2881 ctaccaccgg ctcgggttcg ggcacaggtt ccggttctgg ttcgggaaga aggggtttta
     2941 cattgtaaat atcacttgtt cctccgtcgc ggttgcttgc aaaataagca tcgtttgccg
     3001 agagaataaa ataagcatca aaaccggggg agttgacttt attccccaaa ttctgtggtt
     3061 tcgaccagtt tctccaggtt tcatccatgc ggaaacaaac gaaaatatca gcatctcctt
     3121 caccgccatg accatttgac gaaaagaaaa gtgaatatcc gtcttccgat aaaaacggcg
     3181 acatctcaaa tccttttgaa ttgatagcgt ttcccaaatg aataggaatt ccccatgatc
     3241 catcgggctg acgaagactt acgtacagat cttcctctcc caatgacccc ggaccttcaa
     3301 acgaaatgag aattactgtt tcctgcggat tgacataaaa cccgaaatgg ttaccggtaa
     3361 gtttgaatcc ggcaatttca agtttaacag gatcggaata agtaccgttc ggacgttttt
     3421 ttgcaacggc aattcctctg tcgagttgat tttttttctt gacgtatgca ttgtataaat
     3481 acactgtgtt cccatcgagt gaaacaccaa ctacggcgtt gaaatctttg ttgtttaatg
     3541 ctttcaggtt ttttgcggca tcataaatac cgcctactcc acgcttggag taatagatat
     3601 cctgatcttc atttccacct gtgttatcat cgaaaaactg gcgcacaaaa aacaaagtgg
     3661 aactgtcccg cgaaaatacc agcaaggctt cctctccggg tgaattgatc gtagccggaa
     3721 gtttaaccgg gtctccccag atcaactccg aaacttgtgc gtagctgttt acactcagaa
     3781 taattccaag tgctgttaat gctgttctta cagtgttttt aaatcctgat tctgtaacct
     3841 gcaacaattt catgtccgcc tgaattttgt tttctcaatg atgaaatcgt gtagtcgtag
     3901 gaataaccga tcttgatgtt gttccagaac attccgagca tccccacaat ggcgtctttg
     3961 tgacgatacg agaatcctcc gaaaaacata ttgtcgtaat ccactttgca attcaggtca
     4021 atcgccatcg gtgccgggtt catgtacttc aacaaaaaat ttggagtgat cgaaagtttt
     4081 tcattcacat caaagcgata tcctcccata aagaaatggt gcattttcaa tttcgcgttg
     4141 gtaggatcac cgaaatacaa tttgttttgc agcaattgcg cacttgaata cccaacctga
     4201 aattgttccg agtacagata tacgccggta aatacatcaa acagatttcc gcgtgtggtg
     4261 ttccccagaa attgggcgta ggtattgtca ttgggatcaa gcattgtaac ctttgcctga
     4321 tcgaaaataa cattactcat tcccacacca acaccaatag acgcgttgat tccgtttgca
     4381 acaggaagat gcaaggcata cgaaccgtta aacatattac gcttgaatgc accataacgg
     4441 tcgcccatta cgtttcctcc aacggcatgt ctcatttttc ctgtacggat accggtattt
     4501 ttctgtacgg tttgacgact ggttcgaagc gatgcagaat acttcggact accgcctaca
     4561 tttccaacca cagaatttcc gctcaggtaa aatgtctgag gtgaattttc gaatccaacc
     4621 cactgctgac gaaaactgag gttcaagtcg acaaaatccg tgagtccagc tgctgcagga
     4681 ttgatcacgt atgggttgag cagatactgg ctgagctgtt gcatttgctg agcagatgca
     4741 attccgccag ataccaaaag tgctgatgct ataaagagct ttttcatata caagttcatc
     4801 taacgattaa cgaataatgg ttactgtgcc ttttaccgga tccgatccat cggcaaagtc
     4861 aattacgaag tagtaagatc ctacaggcat tttattgcct ttgaaacttc catcccatgg
     4921 atcagcataa cctgtcgatt cgaaaagttt tgcgccccaa cggttaaata cctgtacggt
     4981 gcaattggga tgataaagat tcaggaatgg aatttcccaa acatcattgg ttccgtcacc
     5041 atcgggtgta aatcctgttg gaatgataat ttcaaaacag gtgctcatta caatcaacaa
     5101 tgaatcggat gttgggggac aagctcctgt tggcgttacc acccaggcta aaacagatgt
     5161 gccgttgctt aggttggtaa cggtagttgt agccgatgtt ggtgtgccaa ttgttcctgc
     5221 tcctgataaa attacccagg ttcctgttcc attgcttgga gcggttgctg taatggtaac
     5281 agtatttacc gtgtcgcaaa caattacatc aggtccggct actgcaggac tcgtggctgc
     5341 atcaactgta accgtaaagc tacaggtaga aatgtttcct gcggcatcgg ttgcagagta
     5401 ggtgagggta gtcgttccaa ttgggaatgt ggatccgctg cttaatcctg ttgcgtctgt
     5461 ttgaactact gtagcagttc cgcaattgtc ggtagctgtt ggtgcaacaa atgttactac
     5521 ctgattacaa ctgttgatgt tggctgtgca attaattaca ggtgcttccg tatcggcaac
     5581 tgtaacagtg aaattacagg tactctgatt tccgttggca tcagtcaatg taagcgtaac
     5641 tgtagttgta cctgcagaaa ttgctgtgcc tgcagcagga ttttgagtta cagttacact
     5701 ggaagatccg caattgtctg tgtaggttac ttgtgatgtg taatcaccga gtgtatgtgt
     5761 acaacccgaa gtgatgttca cgttttgtgg cgatacacag tttgtaacaa caggagcggt
     5821 gttatcagtg agaattacag agaacgtaca cgttgcagaa ttacctgctc cgtcggttgc
     5881 tgttaaggtt acaatcgttg ttccagtaat tactgttccc gccgaaggtg attgcgtaat
     5941 ggttatactt cctgctgcag aacaattgtc gcttactgta gcaagcgtgg tatagcttgg
     6001 cagtgtaaac tgacagttgg catcaggcgc ttcgttttga ttgggcgcac aggttacgaa
     6061 tgttggtgca attgcatctg ctaccgatac agagaaggta caggtattcg catttccgga
     6121 tgcatcagtt gcagttagcg taactgtagt aggaccacta atggttgttc cagccgcggg
     6181 agattgtgtt accgttacac tggcagaacc tccgcagtta tcgtttacaa ttgcggttga
     6241 agtgtaatca cccaatacgc cctgacaact tgcattgccc ggaacagttt ggtttggcgg
     6301 gcaagatgta aatgttggtg cacttgcatc tgttaccgtt accgtaaatg aacagttatc
     6361 gctgttacca tttgcatcgg caactgtgat tgttatggta tgcgtaccgg ttcccaatgc
     6421 agttccggca gcaggtgact gtgtaattgt aatgtttgct ccggatgaac aattgtctgt
     6481 aactgaaata gaagaattga agttgggaac aatatactgg caagacgcat ttacatttcc
     6541 ggtagtgttg gcaggacatc cacctgtaat ggtaggagga gtgttatctg caacgattac
     6601 ctggaatgta cacgtgctaa tgtttccgtt attatcggtt gcctgtatgg tcaccgtttg
     6661 agttcctgat actacagtac ccggaatcgg tgattgtgtg agactgaaca taccgcagtt
     6721 gtcggttgct gttatgccgg acaaataatc tgctattaca aactgacaag atgcgttggc
     6781 agttacgttt tggttaccgg gacatcctgt aataacaggg ttttgcgtgt cggcaacact
     6841 tacctggaag gaacatgttg caatgtttgt tcctgcatct gttgctgtaa tggtgatggt
     6901 atgatttcca acaccaagtg tagttcctgc aacaggtgat tgagaaacaa cgatgcttcc
     6961 cgctggtgta cagttgtcgc tgacagtaat acttgttgtg aagttgggta cagtataagt
     7021 acaacttgca ttaacgttcg cagtttgatt ggaaggacat cctgagatta ccggaacagt
     7081 attgtctgta actgtaaccg tgaatgaaca gttggcattg tttccgtttg catcggttgc
     7141 agtgagtgtg atggtgtgta cacctactcc aagattggaa cctgctgcag gcgattgtac
     7201 taccgtgatg cttccggatg gtgtacagtt atcgctaacc gtaatggaag tcgcaaaatt
     7261 gggaacggtg tactgacacg atgcgttcac gttgccattt gtattggcag gacaacctgt
     7321 aataagcgga gaactgttat cggtaactgt aattgtaaat gaacagttgg cagtattacc
     7381 tgcagcatcg gttgcgttaa tagtgatcga gtgaactcca acaccaagta tggttcctgc
     7441 aaccggcgat tgcgtacgcg tgatagaagc aggtgctgta cagttatcgc taactgttat
     7501 cgatccggtg aaacttggaa cgatatactg acacgatgcg tttacgtttc ctgttgtatt
     7561 agcaggacaa cctgtaatgg tcggtgctgc attatcggtt accgtaacag tgtacgaaca
     7621 tgtggccaca ttgtttgctg catcacgtac agtataggta actgttgtaa ctccggtatt
     7681 gaatgtttgt gttccgatgt tgttgatacc tgcaccggga gagtttccgg ttgtggcacc
     7741 tgtcattgcc caggttaatg ttgtaacacc gcagttatcg ccggttgttg ggttgggtga
     7801 tgttaccgat gcagtacatc ctgcagctcc tgtggttccg gtttggttgg aaggacaggt
     7861 tatggttggg tttatattgt cggtaacggt aaccgtaaat gaacaggtag caggattgct
     7921 ggaagcatca cgcacagtat aagtaacagt tgttactcct gcattgaatg taaatgttcc
     7981 cacattattg attcctgctc cgggcgaatt ggcaacggtt gcgcctgtca tggcccatgt
     8041 taatgtgctc acctggcaat tgtcattggt aacaggattc ggaattgtaa ccgatgctgt
     8101 acacgatcct gcactggtat ttgccgtttg attggaaggg caagtgattg tcggtgctgt
     8161 tacatccggt ggaatggtaa caacaacaga accacccgga cttgtacatc cgtttacagt
     8221 ataggtgagt gtatatgttc ctgcaacaac aggatttcgc gatggatttt gtacgttcga
     8281 agtccatcct ccgggaccgg tccagctata ggtagcaccg gcaatagttg ggacagataa
     8341 attgagtaca ctgtttgaac aggaaaccgg tgcggcactt ggtacgggtg aaggtaatcc
     8401 cactactgct gttccgttaa agtcaagctg gaatcctgca cttggataat catcccattg
     8461 gttgatcaaa aggtaatagg ttcctactgc aaggttagct ggggcaaccc atccgttacc
     8521 attcacatct tccgagttat ctactgctcc gttacccatt ccggtatttc cggtgttgga
     8581 tgcgtaggaa caacgaatcg gtgtgcctaa tcctgaacat ccgccaacga ccggtccgta
     8641 tagcgcaaaa tcgtagtcgg cagcaacagg gtttggtacg atggtcattc cgaatgtgcc
     8701 tggtgtggta acattaatga tataccagtt cgaatagttt tcggcaatta cacaatcaga
     8761 aacacaggca tccgcaataa gtccgggacc ggaggaattt gctgttaccg gattaaggtt
     8821 atcgcaaaca aattgagcgt tgttgcaatc tgcaatggtg ttggattgcg gaccaattcc
     8881 tgcaccaccg gagcaaggga cacaacttaa tgttgcgaac catcctggag tattgatgac
     8941 gttgtcggat aaaaaacgaa cggtgataca accactgggg ttgttggatg tgaatgtagt
     9001 tccggcagga atattacatc cactgaaaag ggttggtccg ttctgagtgg gtgaatcagt
     9061 aattatgaga aaatcccagc acccgaatcc gtcctgatca agtgcaacac tgttctgaaa
     9121 cgtgagtcgc atacattgcc cgggggtatt cggacaaaat gtacggtaga tgttattaat
     9181 gttgttgctg taatttcctg cggcacctcc attatcataa tagtttcctg tacatccgct
     9241 taccatacag tttcctgcat aggtattttg gattccggta gtggggtgaa ggtaggtctg
     9301 tgcaatcgca gccgatgaaa gaaaagcaaa acctatagtg gcgagtaaaa actttttcat
     9361 ggcgattaca gtttttcagg gttaaagaag aggtcaagat cgtctttgtg aatcagcacc
     9421 agataaattt tggtgggaac ctgaaagacg gcttgttttt gttttgcgag ttcctcaaca
     9481 aaaggaaact gaatattcaa tttgcgttgc agcaaaatca cctgttcgat catgcgaaga
     9541 tgacttcttg cctttagcgc tttggcatcg ggtagttcgc gctgttgcac ggaagtaaat
     9601 ttcgggttcg cagctctgtt ctcgagatac tgataaaact ttaccggatt ggcagaacac
     9661 cactgacgca cctgttcaag gtacacttca acattgggtg cagttacgcc atttcccaga
     9721 cgcggataag tcggatcatc aattccgatc agctccatgt aaaaatcagg gtaaccacgc
     9781 tcgggttgat tggcatcttt ttgagccgaa accgaaacgg agaacaaact gatgacaagt
     9841 gccaacagta atttgtaatt cttcatgatt gaaaaattag tgggttagaa tagttgaaaa
     9901 aacctatgta gtgagtaggt cgcatataga cctgcggcaa gcagtgacac aaaagtaaag
     9961 aaattgtgaa ttaatctgca agaccaaaat gcttcgtcga tgactaaaac cgatttagtg
    10021 aagaaatagt cgtatttatc gataaaagtt gaatatttca actcatcggt ttgttgttga
    10081 aaactagttc attacctctt aaacattagc ttttgagtag aaatacgcaa aacgtttatt
    10141 tttcaaaaag aagagattgg aggattccaa atagacagga taaaaagtgt cttgttctct
    10201 aatggtaaga ttaataggtg tatgattttg tttggggctt tgagtagtta tacgcagaaa
    10261 aagcgagtat atatactcaa atatttgagt atatgtaccc aaaatagtga gtagatgtac
    10321 tcaagtgtaa tgagaagtta agcgcagaag tattgagtgt atatactcaa ttatttgagt
    10381 agaagtactc aatactttcc caagatattc tttaccaggt atttgtattc aaccaaatga
    10441 gcacaaatac gcaggacttt tgagtagaag tactcaaatg ggtaaaaaaa aacaggcatc
    10501 ctgttaaggg atgcctgttt ttatgaattg tttgtagcgg cttagtgagc agcctcttca
    10561 gtagcaggag ctgtagtgtc agcaggagct tcaactggtg caggttcttc aactggtgca
    10621 ggagcagcag ttgctgtaga atcagcagct ttttcatcag ttttagtttc tccaccgcaa
    10681 gcaacaagtg ccacagcgat aaggctagcg aaaattactt ttttcatttt tttaggtttt
    10741 agggtttgat tttacatcta tatacccaaa taccgatgga ggtaacccat attcaaaaaa
    10801 aaaaattggg ttacct
//
LOCUS       DAOMYG010000034        10779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2037_length_10779_cov_11.075625, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000034 DAOMYG010000000
VERSION     DAOMYG010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10779
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2037_length_10779_cov_11.075625"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="PK610_02020"
     CDS             complement(<1..1250)
                     /locus_tag="PK610_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34728.1"
                     /translation="MKHLIVVIFVFLFGSAISQRVVYLEYVPESAPGAGDNKLIAHNS
                     GLTQPVQFNWYLGGYGPRGNTSIIENITPGSYSVTVIDSACFTFYATMYFTSNAGSVR
                     MVENVEAVLPTPGNSDGSIRFTRRNYQVTDEMAFGPTGGSQLTTTDTAFFNLPEDQYT
                     FNITPAGSPFYGVRNVVNLQNQPLNSPCYHFDKTLVVQPPSNFCIGQINVTGSNSPET
                     NFEYFINVHDFSMGSAIQMNSNSAFGAAYGLCTGFYSIETRNWNNKMQYTDYLFLQPS
                     SSTPYVWNNPDSILPGTDTVVLFPTMNCGIVQGPPIDTMFISSIVSLGSGQFQFSVAV
                     VQAGDTSFIPAETQVDTTQNFFVDLSMFCVDTSRTVTVFERYMVYWGERIMLSVPSFT
                     NSSSVRLFPNPSRENVMLEFDLSS"
     gene            complement(1262..4039)
                     /gene="leuS"
                     /locus_tag="PK610_02025"
     CDS             complement(1262..4039)
                     /gene="leuS"
                     /locus_tag="PK610_02025"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRJ34729.1"
                     /translation="MEYNPREIEQKWQQYWATNKTFTASNNSELPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIIARYKRLQGFNVLHPMGYDSFGLPAEQYAIQTGQHPALTTEQ
                     NIARYRQQLDRIGFSFDWDREVRTSDPAYYRWTQWIFIRLFDSWYNRQNDKAEPISQL
                     VSIFEQHGFQGAEAQVLTGGIERDVQAFSADEWKTFDEKKKMEVLMNFRLAYLDDSWV
                     NWCPALGTVLANDEVKDGVSERGGHPVERKLMKQWSLRITAYSERLLRDLDTIDWSDS
                     IKEAQRNWIGKSEGASIRFDLVGHAETIEVFTTRPDTIFGVSFVVLAPEHELVSKITT
                     SQHKAVVENYITYAKNRSERERQAEVKNVSGAFTGAYVKHPFTGKLVPVWIADYVLAG
                     YGTGAVMAVPCGDQRDFVFAKHFDLPIPAIMEGVDISEKADPTKEAKLINSDFLNGLT
                     GYEAIRVCIAKIEASGFGKGKVNYRLRDAIFGRQRYWGEPIPVYYKDGIPYCVEENDL
                     PVTLPEVDKFLPTETGEPPLARAANWKHQGKYEYETTTMPGWAGSSWYFLRYMDPKNQ
                     SAFASREAMAYWNQVDLYIGGAEHATGHLLYFRFWTKFLMDFGFIEFAEPAKKLINQG
                     MIQGVSKIVYRINGSNKIVSRNLRDKYETSPIHVDLKLADGDVINVEELKKWRDDFAS
                     AEFILEEGQLLCTSEVGKMSKRWHNVVNPDDLCDSVGADTLRLYEMFLGPLEQHKPWD
                     TKGIEGTSRFLKKLWRLFHNENGFAVSDETASPEELKALHKAIKKVVEDIDRYSFNTV
                     VSTLMICVNELSDLKCNKRSVLSELVVLVSPYAPHIAEELWSKLGNRNSVSFAKLPEF
                     NPAFLVEDSITYPIQFNGKLRFNLDLPSALTPAEIEKEVLANEQTQKYLEGKLPKKII
                     VVPKRIVNLVV"
     gene            4129..6705
                     /locus_tag="PK610_02030"
     CDS             4129..6705
                     /locus_tag="PK610_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ34730.1"
                     /translation="MKRLLLLAAMLFAAQQSYAACSGMTTNINSIPASCFGQCNGAAQ
                     VFVTGGSGNYNYFYLDLSNNAVGTSNANQVTGLCAGNFKVVVQDITNSCFDTTNFSIS
                     QPPMLVSTAFITQGISCFGASTGVIQSAASGGTPPYMFMWSNGVTTPINPGVQAGAYI
                     LQVVDANGCQAMPFTVVMNQPTAISINTSSTPSSCSSPDGTATATAIGGTPTFVYSWN
                     TMPVQTTATATGLAAGTYIVTATDANGCTATQNVTVSGSGSLVVTANATGSSCNPCTG
                     QVFAIAANGTAPYEFSLDGGPQQSGTLFTNVCPGPHTVTAFDDAGCMAVYTVIVPNNG
                     VPGLSVSEMMSPESGIGMQNGILDLTLTGGNGPFTFLWSNGATGEDIYSLTGGVYSVT
                     ITDVNGFCATYAFQVTTLTGYGWVTGTLINDANGNCVFDSGEFPLANYLITLTNGTQS
                     YFGYTNSSGQYSIWAPTGNYTLQPSNTTNLSAACGSSINVAVPNGATAGNNNFYYQIP
                     PVHDVCVNVWSSAMVPGFNGYYAVTVTNYGSLTSSGDVCLPLPAAINYLNSSITPNST
                     NGDTICFSYSNLNPGATFVVYVNFFTPIGTTLGSVTIACANATVTNGTDINPGCNTFC
                     YTRVVTGSYDPNDKSVSPSGIGSNGDIEVSEDKFTYLIRFQNTGNGPAVNIFITDTLS
                     SLLDPTSIVMLNASHDYSIDILPGNIIRWHFANIMLPDSTSDEPNSHGHVQFTINTMN
                     TPQIGQSIENTANIYFDFNEPVITNTTINTYADFSSVTEHGTESILLYPNPAGDRFSI
                     RTKDNNPVQAELIDISGRIIRTQQLRNGDSFQTADCANGVYLVRLTANDTQQTIRLVV
                     QH"
     gene            6833..7723
                     /locus_tag="PK610_02035"
     CDS             6833..7723
                     /locus_tag="PK610_02035"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRJ34731.1"
                     /translation="MSQESNRLARRSTRASYISTVIGISLVLFMLGLVGWGVMISRKV
                     TRLAKESIRMDVFFKENVRDADMRQLEKTLASEDWVKSARFVSKEEALSITESILHDD
                     SLTAPVDNYNAILPSIEVYINEEWAQSDSVIKIEDALLAKYKTTLIEEVWYDRSMFLT
                     INNSTKYAAWIILALAGLLLVVAIALINNTIRLAIYAKRLLIRSMQLVGATESFIRRP
                     FLFRAFVQGILSSLISLGLLLLGMNYLITWFPDLTEVHDTPMMLMLFGGITVLGILIS
                     WISTYFALRKYLRLKSELLY"
     gene            7726..7980
                     /locus_tag="PK610_02040"
     CDS             7726..7980
                     /locus_tag="PK610_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HRJ34732.1"
                     /translation="MSRHEQPTQIPFTRENYYFIIAGVVLVILGFMAMIGGGSTEANE
                     FNEAELFSFRRITLAPFLVILGYVVVLVGILRRPKNENKV"
     gene            7981..8772
                     /locus_tag="PK610_02045"
     CDS             7981..8772
                     /locus_tag="PK610_02045"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRJ34733.1"
                     /translation="MELLDAIIIAVVEGITEYLPVSSTGHMIITEKVLGLESTDFSKA
                     FMINIQLGAILSVVVLYWKRFFQSLGFYFKLITAFIPAAVLGLLLSDYIDSMLESVVT
                     VACSLVLGGMILLFIDKWLDKAENNAEVDYPKAFKIGLFQCIAMIPGVSRSAATIIGG
                     MTQKLNRKTAAEFSFFLAVPTMLAASAKKLKDVWDKDPSILTENINTLLIGNLVAFVV
                     AMIAIKFFIDYLSKYGFRVFGYYRIIVGATILALLAMGYELNITG"
     gene            8769..9512
                     /gene="truB"
                     /locus_tag="PK610_02050"
     CDS             8769..9512
                     /gene="truB"
                     /locus_tag="PK610_02050"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRJ34734.1"
                     /translation="MKIDLQQGEIWLVDKPYEWTSFDVLRKLKPALIRNTGDKKAKIG
                     HAGTLDPLATGLLIICVGKATKKIDTLQSGIKEYTGSIFLGATTPSYDRETEVNAQFP
                     ISGIGEKEIRETAQIFLGKQEQQAPVFSAKMIDGKRAYDHARKGTEIEMKKHEVEIYG
                     FEITSIELPLIHFKVTCSKGTYIRSLAHDFGKRLNSGGYLFDLRRTKSGNFDVENAET
                     LQQLLERLSGEKHEIELDSKRAFLRDNRK"
     gene            complement(9539..10495)
                     /locus_tag="PK610_02055"
     CDS             complement(9539..10495)
                     /locus_tag="PK610_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34735.1"
                     /translation="MENNKPKKSRDAIYITLILLLLGGGGLLFVQMSQYKTNYEQYKQ
                     NFETCAQEKVEMEKLFNENGMADMLGSDLKEGLANLMEDYQSLETSNTGMRDSIQRQI
                     RKIDSLQKEAEKAKGNAYAIYRLKKETETLRSIMQGYVRTIDSLNTINQELRADLSTT
                     KKNLNNVEKERDDFKEKTKDLEGKVAKGSILQTANFTAGAIHLRNSGKQVETTRAGRA
                     DMVKACCTILENSIAKPGTKNVYMVVTQPDATILTQNPEQTFKWEGGESQYSLLREVD
                     YQNQAIDICFYADVKTDLPKGVYIIELFCEKAKIGKTTFTLK"
BASE COUNT         3042 a         2463 c         2144 g         3130 t
ORIGIN      
        1 ttggaagaaa gatcgaattc aagcattaca ttttcccggc ttgggttggg aaaaagtctt
       61 accgaagatg aattggtgaa tgacggaaca gataacataa tcctttctcc ccaatacacc
      121 atgtagcgtt caaaaacggt aacagttcgg gaagtatcga cacaaaacat ggagagatct
      181 acgaaaaaat tctgagtggt atcaacctgt gtttccgcag gaataaatga agtgtctcct
      241 gcctgaacaa cagcaacaga aaactgaaac tgccccgacc ccaatgaaac gatggaggaa
      301 ataaacatcg tatcgattgg cggaccttgt actattccac aattcatcgt tgggaataaa
      361 actactgtat ctgttccagg caaaatggaa tcaggattgt tccaaacata aggagtagac
      421 gatgaaggct gaaggaataa ataatcggtg tattgcattt tgttgttcca attcctggtt
      481 tcaatcgaat aaaatcctgt acataaacca taagcagcac caaatgcaga attagaattc
      541 atctgtatgg cactacccat ggagaaatcg tgaacattaa taaaatactc gaagtttgtt
      601 tcgggactgt tacttcctgt gacgttgatt tgcccaatac aaaaattact tggaggttga
      661 acgacgagtg ttttatcaaa atggtaacat ggcgaattga gtggttgatt ctgcagattt
      721 acaacattgc gcacgccgta gaatggagag cctgccggtg taatattaaa tgtgtattga
      781 tcttccggca aattaaaaaa tgctgtatca gtagtagtca attgcgaacc accggtaggt
      841 ccgaatgcca tttcatccgt tacctgataa ttgcgcctgg taaaacgaat actcccatcg
      901 ctattaccag gagtaggcag tactgcttca acattttcaa ccattcgaac tgatcctgca
      961 ttactggtaa aatacattgt tgcgtagaat gtaaaacaag cagaatcaat tacagttaca
     1021 gaatacgaac ccggtgttat attctcaatg atggacgtat tccctctcgg accgtaacca
     1081 ccaagatacc aattgaactg aacaggctgt gttaatccgg aattatgcgc aatgagttta
     1141 ttatcgccag caccgggcgc cgattcagga acatattcca aatacacaac cctttgagaa
     1201 attgcactcc cgaacaggaa aacaaaaatc actactatta aatgcttcat gtatgtcatt
     1261 tttaaactac cagattcaca attcgtttgg gcacaacaat tatcttttta ggcagtttgc
     1321 cttcaagata tttttgtgtt tgttcgttgg ccaacacttc cttttcaatt tctgcaggag
     1381 ttaatgctga tggcaggtca agattaaaac gcagtttccc attgaactga atgggatagg
     1441 taatactgtc ttcaaccaga aatgcaggat taaactccgg caattttgca aaacttacgc
     1501 tgtttctatt acccaatttg ctccatagct cttccgcaat atggggtgca taaggcgaaa
     1561 ccaaaacaac cagctcactt aatacagacc gtttattgca tttcaaatcg cttagttcat
     1621 ttacacaaat catcaatgta gaaactacag tattgaaaga atatctgtcg atatcttcca
     1681 ccactttttt aatagccttg tgcagcgctt ttaattcctc cggacttgca gtttcatcgc
     1741 tcacagcaaa tccattttca ttatggaata aacgccataa ctttttcaaa aagcgggaag
     1801 tcccttcgat tcctttagta tcccagggct tgtgttgttc gagcggacca agaaacatct
     1861 catacaagcg caaggtatct gcacctaccg aatcgcagag atcatcggga tttacaacat
     1921 tgtgccaacg cttcgacatt tttcccactt cactggtgca tagcagctga ccttcttcaa
     1981 gaataaattc tgctgatgca aaatcatctc tccatttttt taattcttcc acattgatca
     2041 catctccgtc tgcaagcttc aggtcgacat gtattggaga tgtttcgtat ttatcgcgaa
     2101 gatttcttga aacaatttta ttggaaccat tgatccggta aacaatttta gaaacgccct
     2161 gaatcattcc ctgattgatc agtttttttg ccggttctgc aaattcaata aaaccaaaat
     2221 ccatcaggaa ttttgtccag aaacgaaaat aaagtaagtg tccggtcgcg tgttctgctc
     2281 ctccaatata aagatcaacc tgattccaat aagccattgc ctcacgcgac gcaaatgcag
     2341 attgattttt gggatccata taccggagaa aataccatga actacccgcc caacccggca
     2401 tggtggtggt ttcgtattcg tatttcccct ggtgtttcca gtttgcagcg cgcgctaacg
     2461 gtggctcacc tgtttcagta ggtaaaaatt tatcgacctc cggtagtgtt acgggcagat
     2521 cgttttcttc aacacaatac ggaattccat ctttgtaata aaccggaatg ggttctcccc
     2581 aatacctttg tcttccaaaa atcgcatcgc gcaaacggta atttactttt ccttttccga
     2641 atccggacgc ttcaattttt gcaatgcata cgcgaatagc ttcatatccg gtaagtccat
     2701 tcaaaaaatc ggaattgatg agtttggcct ctttggtagg atctgccttt tctgaaatat
     2761 ctactccctc cataattgca ggaataggaa ggtcaaaatg ttttgcaaac acgaaatcac
     2821 gctgatcgcc acatggaact gccataacag cacctgttcc gtaacctgca agtacatagt
     2881 cagcaatcca aaccggaaca agtttgcctg taaatggatg tttcacataa gctccggtaa
     2941 aagcaccact tacattcttt acctctgcct gacgctcacg ctcacttctg ttttttgcat
     3001 aggtaatata attctctaca actgccttat gttgtgaagt tgtgatttta gaaaccagtt
     3061 cgtgttcagg cgcaagtacc acaaacgaga caccgaaaat agtatccgga cgcgtagtaa
     3121 atacttctat cgtttcagca tgaccaacaa gatcaaaacg aatgcttgct ccctctgatt
     3181 ttccaatcca gttgcgttgt gcttctttaa tcgaatcgct ccagtcgatg gtatcaagat
     3241 cgcgcaacaa ccgctccgaa taggccgtaa tgcgtaaact ccattgcttc atcaactttc
     3301 gttctacagg atgtcctccc ctttcggaaa cgccgtcttt tacttcatcg ttagcaagca
     3361 ctgtacccaa agcgggacac cagttcaccc aactgtcgtc gaggtaagca aggcgaaaat
     3421 tcataagaac ctccatcttt tttttctcgt caaacgtttt ccactcatct gcagaaaagg
     3481 cctgaacatc gcgttcgata ccaccagtga gtacttgcgc ttctgcaccc tgaaaaccgt
     3541 gttgctcaaa aattgaaacc aattgtgaaa tcggttcggc cttatcgttc tgtcggttgt
     3601 accatgaatc aaacaaccgg ataaagatcc attgtgtcca tctgtaataa gcaggatcag
     3661 atgttcgcac ttcgcggtcc caatcgaacg aaaatcctat tctatccaat tgctggcggt
     3721 aacgtgcaat attctgttcg gtagtaagtg caggatgttg tcctgtttga attgcatatt
     3781 gttctgcagg caatccaaac gaatcgtatc ccatcggatg aagaacattg aagccctgaa
     3841 gtcgtttgta tcgtgcaatg atatcggaag ctatgtaacc aagcgggtga cctacatgca
     3901 aaccagctcc tgaaggatat ggaaacatat ccaaaacata atatttcgga agttctgaat
     3961 tattggatgc ggtaaatgtt ttattcgtag cccaatactg ctgccatttt tgttcgatct
     4021 ctcttgggtt gtattccata gttttcaatt gaaacgacga aattaggaaa aaaggctcag
     4081 gcaaccttca tttaagggct gcgtctaacc ttcaaaaccc tccaaaccat gaaacgcctt
     4141 ctactattgg cggcaatgtt atttgctgcc cagcaatctt atgccgcttg ttccggtatg
     4201 accaccaata tcaactccat tcctgcttct tgtttcggac aatgcaatgg cgccgcacaa
     4261 gtatttgtga ctggtgggag tggcaactac aactacttct atttggacct aagtaataat
     4321 gccgtgggta cttcgaatgc caatcaggta acaggccttt gcgccgggaa ttttaaagtt
     4381 gttgttcagg acattactaa ttcatgtttt gacacaacaa acttttctat ttctcaaccg
     4441 cctatgttgg tgagtactgc ctttatcacc cagggcatct cctgttttgg cgctagcact
     4501 ggtgttatac aatctgcagc atctggaggt actccaccct atatgtttat gtggtcgaat
     4561 ggtgtcacaa ctccaatcaa tccgggggtt caggccggag cttatattct tcaggttgtt
     4621 gatgccaatg gatgtcaggc aatgccattt accgttgtga tgaatcaacc aacagcgatt
     4681 tcaatcaata caagttcaac cccttcatca tgcagcagcc cggacggtac tgctacagca
     4741 actgccatcg gtggtacacc aacatttgtt tactcctgga acacaatgcc agttcaaacc
     4801 acagcaactg caacaggcct tgctgcagga acctatattg ttactgcaac agatgccaat
     4861 ggttgcaccg caactcaaaa cgttactgtt agcggttccg gatcattagt agttaccgcc
     4921 aacgcaacag gaagtagctg taacccttgt accggacagg tttttgctat cgccgcaaat
     4981 ggaactgctc cctacgaatt cagtcttgac ggcggtccgc aacaaagtgg aactttattc
     5041 accaatgttt gtcccggccc tcatactgta actgcatttg acgatgcagg atgtatggct
     5101 gtatataccg tgatcgttcc caacaatggt gttcccggac tttctgtcag cgaaatgatg
     5161 tctcccgaat ccggtattgg catgcaaaac ggaattcttg atctaaccct tacgggagga
     5221 aatggtcctt tcactttttt atggtcgaac ggagcaaccg gagaagacat ttactctctt
     5281 acaggaggcg tttactcggt tacaattaca gatgtaaacg gattttgtgc gacttatgct
     5341 tttcaggtta caacattgac aggatatgga tgggtcacag gtacactcat taacgatgct
     5401 aacggaaatt gtgtttttga ttccggagaa tttcccttgg caaattatct tattacactt
     5461 acaaacggaa cacaatcgta ttttggatac acgaattctt ccgggcagta ttcgatttgg
     5521 gcgccaaccg gaaactatac tttacaacca tcaaatacaa caaatctctc tgcagcttgc
     5581 ggtagttcaa taaatgtagc tgttcccaat ggcgctacag caggaaataa taatttttat
     5641 taccagattc ctcctgtaca cgatgtttgt gtaaacgttt ggtcctccgc aatggtaccc
     5701 ggatttaacg ggtattatgc agtaactgtt accaactacg gctcactcac ttcgagcgga
     5761 gatgtttgcc tgcccttacc tgctgcaatt aattacctca acagttcaat tactcccaat
     5821 agtaccaatg gagatacgat ttgcttctct tattccaatc tgaatccggg agctactttt
     5881 gttgtttacg tgaacttttt cactcctatt ggaacaacac ttggcagtgt aactatcgct
     5941 tgcgccaacg ctactgtcac caatggaaca gacatcaatc cgggttgcaa tacattttgc
     6001 tacacacgcg ttgtaacagg ttcttatgat ccaaacgaca aatcggtatc gccttcagga
     6061 attggaagca atggcgacat tgaagtgagc gaagacaagt tcacgtacct catccgtttt
     6121 cagaatacgg ggaatggtcc tgcagtgaat attttcataa ccgatacgct ctcttccttg
     6181 cttgatccaa cttccattgt aatgctcaat gcgagtcatg attattcaat cgacattctt
     6241 cccgggaaca tcatacgctg gcattttgcc aacatcatgc tacccgatag caccagcgac
     6301 gaacccaaca gtcatggtca tgtacagttt accattaaca ccatgaacac gccacaaatc
     6361 ggacaatcca tcgaaaacac cgctaacatc tatttcgatt ttaacgaacc tgttattacc
     6421 aataccacga ttaacacata tgcagatttc tcatccgtaa cggaacatgg aacagagtcc
     6481 attctcctct atccgaatcc ggcaggcgat cgttttagta tccggaccaa ggacaacaac
     6541 cctgttcagg cggagttgat cgatatttcc ggaaggatta tacgcacgca acagcttcgc
     6601 aatggtgatt cgttccaaac agctgattgt gccaacggag tttatctggt tcgccttaca
     6661 gctaatgata cgcagcaaac cattcgtctg gtagttcaac actgatcaat atttatttaa
     6721 tgaaccaaag ccactccaat tggagtggct tttttacgtt attaacatcg cgctattggt
     6781 ctttcaggat ttttaacagc tcatcaatcc tactaatctc tattttcgct gcatgagtca
     6841 ggaaagcaat cgcctcgccc gccgtagcac acgagcatcg tacatctcaa cggtgatcgg
     6901 aatttcgctg gtgcttttta tgcttgggct tgttggctgg ggagtaatga tctcccgcaa
     6961 agtaacacgt ctggccaagg aaagtattcg tatggacgta ttcttcaagg aaaatgtacg
     7021 tgatgccgat atgcgtcagc tcgaaaaaac acttgcctct gaagactggg tgaaatcggc
     7081 tcgttttgta agtaaagaag aagcactatc cattaccgaa agcattctgc acgatgattc
     7141 gcttactgct cctgtagata attacaatgc gattttacct tccattgagg tttacatcaa
     7201 tgaagaatgg gcccaaagcg acagtgtgat aaagattgaa gacgccctgc ttgccaaata
     7261 caaaaccaca ttaattgaag aagtctggta cgaccgttcc atgtttctca ccattaacaa
     7321 cagcactaaa tatgccgcct ggatcatttt agcactggca ggattattac ttgttgtggc
     7381 cattgctttg attaataaca cgattcggct cgctatttat gccaaacgac tgctcatccg
     7441 gtcgatgcag ctggttggtg caacagagag tttcatccgt cgtccctttt tattcagggc
     7501 ctttgttcag ggcatccttt cgtcacttat ctccctggga ctcctcctgt tgggcatgaa
     7561 ttaccttatt acctggttcc ccgatcttac agaagttcat gataccccga tgatgctgat
     7621 gctatttgga ggcattaccg ttctgggtat ccttatttca tggatttcta cttattttgc
     7681 actgcggaag tacctccgcc tcaaatctga attattgtac tgagcatgag ccgtcacgaa
     7741 caacctaccc aaatcccttt tacccgggaa aattattatt tcattattgc cggagttgtg
     7801 ctggtaattc ttggctttat ggccatgatt ggcggtggat caaccgaagc caatgaattt
     7861 aacgaagccg aactgttttc cttccgtcgc attacccttg caccatttct ggttatttta
     7921 ggatatgtgg ttgtattagt tggcatcctt cgccgtccga aaaacgagaa taaagtctaa
     7981 atggaattgc ttgatgcaat tatcattgct gttgttgaag gaataaccga ataccttcct
     8041 gtttcttcta caggacacat gatcatcacc gaaaaagtac tcggactgga aagcactgat
     8101 ttttcaaaag cttttatgat aaatattcag ttaggcgcca ttctctcggt agtggtgctc
     8161 tactggaaac gtttttttca gtcgctcggt ttctatttca aactcatcac agcatttatt
     8221 cctgcagcag ttctaggttt gttgcttagc gattatatcg actccatgct tgaaagcgtg
     8281 gtaacagttg cctgttcatt ggtcttggga ggcatgatcc tgttgttcat agataaatgg
     8341 ctggataaag cagagaacaa tgcggaagta gattacccca aggcatttaa aatcgggttg
     8401 tttcagtgca ttgctatgat tcccggtgta tcacggtcgg ctgcaaccat tattggaggt
     8461 atgacccaaa aactaaatcg caaaacagcg gcggagtttt cattttttct tgcggtaccc
     8521 actatgttgg ctgcttctgc aaaaaagctg aaagacgtat gggacaaaga cccctccata
     8581 cttacggaaa acatcaacac tctactcatt ggcaatctgg ttgcgtttgt tgttgcgatg
     8641 attgcaataa aattttttat tgattacctc agcaaatatg gtttccgtgt atttggttat
     8701 taccgcatca ttgtaggagc aaccatactt gcattattgg caatggggta tgaattgaat
     8761 attaccggat gaaaattgat ttgcagcaag gagagatctg gctcgttgac aaaccctacg
     8821 aatggacttc ctttgatgtt ttgcgcaaac tcaagcctgc gctaatccga aataccggcg
     8881 ataaaaaagc aaaaattggt cacgcaggaa cactcgatcc gctcgcaacg ggactgctga
     8941 ttatttgcgt tggaaaagca accaaaaaga tcgacacact gcaatccgga atcaaagaat
     9001 acaccggcag tattttttta ggcgcaacaa caccttccta cgacagagaa acagaggtga
     9061 atgcacaatt tcctatttcg ggaataggcg aaaaagaaat acgagaaact gctcaaatct
     9121 ttttaggaaa acaggagcaa caagcaccgg tattttcagc caaaatgatc gatggtaaac
     9181 gagcctacga tcatgcccgc aagggaacag aaatagaaat gaaaaagcat gaagtggaga
     9241 tctacggatt tgagatcaca tcgattgaac ttccactcat tcattttaaa gttacatgca
     9301 gcaagggcac ttacatccgt tcgctggcgc atgattttgg caaacgactg aattcaggag
     9361 gctatctttt cgatcttaga agaaccaaaa gcggaaactt cgatgtagaa aatgccgaaa
     9421 cacttcagca actgctcgaa cgattaagcg gcgaaaaaca cgagatagag cttgatagta
     9481 aacgtgcctt tcttcgcgat aacagaaaat aaaatgcgaa ctaatttacc tgaaaagatt
     9541 acttcagtgt aaatgttgtt ttcccgattt tcgctttctc acagaacaat tcaattatgt
     9601 aaacaccttt gggcagatcc gtttttacat ctgcataaaa acagatatca atcgcctggt
     9661 tctgatagtc tacttcacgt aataaggaat actgactttc gccaccctcc catttaaagg
     9721 tttgttcagg attttgtgtt agaatggttg catccggttg tgttaccacc atgtaaacat
     9781 tttttgtgcc cggcttggca atggaatttt caagaatggt gcaacaggcc ttaaccatat
     9841 ctgcacgtcc tgcccgcgtt gtttctacct gtttacctga attgcgtaaa tgaattgcac
     9901 ccgcagtgaa gttagccgtt tgcagaatag aacctttggc aactttgccc tccaggtctt
     9961 ttgttttctc cttgaaatca tccctttcct tctccacatt attcaggttc tttttggtag
    10021 tgctcaaatc agcacgcaat tcctgattga tggtatttaa cgagtcaatg gttctaacat
    10081 aaccctgcat aatggaacga agtgtttctg tttccttttt caatcggtaa atagcatagg
    10141 catttccctt agctttttcg gcctcctttt gaagggaatc aatcttgcgg atctgacgct
    10201 gaatactatc acgcataccg gtgttggaag tctctaaact ctgataatcc tccatcagat
    10261 tggcaagacc ttctttcagg tccgaaccaa gcatatccgc cattccgttc tcgttgaaca
    10321 acttctccat ttccaccttc tcctgagcac aggtttcaaa attttgcttg tattgttcgt
    10381 aattggtttt gtactgactc atctgcacaa aaagcaatcc tcccccgccc aacaacagca
    10441 gaattaaagt gatgtaaatg gcatcacggg atttctttgg tttgttattc tccataagac
    10501 actggtttca cgacaaaaat acaagcgtat tggcatcaaa aacgaagtgt gggtgaaaat
    10561 gttacagaat ggcttgttca taatgttgca gaatgttaaa atccggcaca aacaagacct
    10621 accgccgaaa cttaatcaag agtataaagt tggtctgcaa gagtctgatt ccctttttag
    10681 gtttgcgtca tgatcaggga aatcgtctca ctcttttatc catcactgtg cgcggcctgt
    10741 ggtgagcctc tattacgcaa tgaaaaaacg atctgcatc
//
LOCUS       DAOMYG010000035        10656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2064_length_10656_cov_9.683520, whole genome shotgun sequence.
ACCESSION   DAOMYG010000035 DAOMYG010000000
VERSION     DAOMYG010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10656
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2064_length_10656_cov_9.683520"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PK610_02060"
     CDS             <1..185
                     /locus_tag="PK610_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HRJ34736.1"
                     /translation="LFLALSESGMNSSTVIPVNNIGIVVASALAGLLLFKERYSLLNK
                     VGLMLSIAAILMISFG"
     gene            191..793
                     /locus_tag="PK610_02065"
     CDS             191..793
                     /locus_tag="PK610_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HRJ34737.1"
                     /translation="MVYSFKSIVASCEGLYKEKGSKFHAYAFPVANEEEIKQHLNELR
                     KKHFDARHHCYAFRLGPDGTHYRANDDQEPSGTAGKPIHGQLLSFGLTNILLVVVRYF
                     GGTKLGASGLIHAYKSAAEDALKQCVVVEHDVMKAIELEFPYERYNDVMRFVRETHSK
                     IIQQQLEEPSRIQLEIPLKNEQTLHDRCERYHEIKFIEEQ"
     gene            790..1722
                     /locus_tag="PK610_02070"
     CDS             790..1722
                     /locus_tag="PK610_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRJ34738.1"
                     /translation="MNDFELLKKMCSIHAPSGDESAMTDFLLSYIKKESKNWKHKVTV
                     YSGPEFQDCIILVFGKPRTTIYAHIDSIGYTAGYENNLLRIGGPQPGEGDRLVGKDAK
                     GKIECELVVIDHEDGGRGLNYLFKRKIERGTSLTFKPIWRESKEYIQCCYMDNRLGVY
                     NALKVAETLKDGAIVFSTYEEHSGGTVGALARFLLEKYKVRQSLISDITWVTHGVKHG
                     KGVAISMRDSGIPRQSYVRRIIDLAKKSKIPFQLEVESAGGSDGTAIQKSDNLIDWCF
                     IGAPEDNVHTPHEKVHKKDIAAMISLYSYLMKKL"
     gene            complement(2200..2640)
                     /locus_tag="PK610_02075"
     CDS             complement(2200..2640)
                     /locus_tag="PK610_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRJ34739.1"
                     /translation="MAQAKENDIVRVHYTGRLSDGVVFDSSEGNEPLEFVIGSGMLIP
                     GFEDSVIGMALNESKTINIPADEAYGPVMDEMIQKVPREHLPEDMQPEVGMELVSETP
                     DGHQLIVVVREVGLEHITIDANHPLAGKDLSFDIRLVEILEPIA"
     gene            2836..3558
                     /locus_tag="PK610_02080"
     CDS             2836..3558
                     /locus_tag="PK610_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRJ34740.1"
                     /translation="MANPKAELNFLDGPRSRWKELGFAIRVFLEFIKGFRSLHFSGPC
                     VTVFGSARFKEGHPSYEMTREISAEMVKLGFTILTGGGPGIMEAANRGAKEAGGRSIG
                     CNIELPFEQHHNPYLDRWVTMRYFFVRKVLLTKYSYAFVVMPGGFGTLDEFFEAITLI
                     QTKKLKPFPIVIMGVEYHKHLLEHIDLMIKHGTISQEDMKLYCVTDSVSEAAKFIEEH
                     SVIKYDLKDIEHPKPNPILGEN"
     gene            3654..5345
                     /locus_tag="PK610_02085"
     CDS             3654..5345
                     /locus_tag="PK610_02085"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRJ34741.1"
                     /translation="MRNLILLLIALISLRNDTIAQQKKPVVELIELDNLVGYYGQDKK
                     FVVLFEQARIIKKFEHVWLVTYGYEFDAQHGESLGHVRSDDVTDTHGKLINRQTLVNA
                     EGINILPGGADHFRYSGNAFIANRGRNFMLFDAKGKKLIDNTEAILTFAGGYAAYKQQ
                     GSWSFIHVSGKIVERNYSELRPLIHECGGYIVRAKNYYSFIDTLLQPVIVGEFTSMQW
                     ISYDHYVVEKAGKYGVINSHDKFLLPVEFERLNANQFLNEEIVIARRNGKDQLLSPWF
                     KFIGRPYDHIKKSETGFYEVRENTSYGIIDSNGREIVAPEYEVCDMISEWGVRVKKGI
                     YYGLISWQGKTILPLKYTTLGLPANERIVLGITDTLSGKSSFTYCDKTGELLSGFNYE
                     SARSFSEGLAAVRKNGKFGFIDTTGKLVVTYQFVEASSFKNGYCVVAKKAGKYGMIDI
                     TGLMIISFKYEKLDDLSEDMIGFRKNGKWGFLNANGEQVIAPQYSEILSFSEGMAAVK
                     DGPYWIYINKNKDLLIDMRIVRAESFSDGNAVIRIENSGRFLIDRNGHIIREIKP"
     gene            complement(5444..5905)
                     /locus_tag="PK610_02090"
     CDS             complement(5444..5905)
                     /locus_tag="PK610_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34742.1"
                     /translation="MQCKRIFIFLFSALVLFSITSCRKRVPAQSGLIGTIAIGPCTFR
                     IPAMAYVANKNAVANQGRNIIWVCGGKKLRENGGYSIIFLEPGAEIVSKGGGNTIYAK
                     SGSKIDSRGGGDVIYYESGTNIVNLKGGSEQLVECPSLIFDYSLAPENGCR"
     gene            complement(5915..7915)
                     /locus_tag="PK610_02095"
     CDS             complement(5915..7915)
                     /locus_tag="PK610_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HRJ34743.1"
                     /translation="MSATANPVLPFDRKTYSNDQLIELYNAILLPRLIEEKMLILLRQ
                     GRISKWFSGYGQEGISVGTAMAIAKEEYILPMHRNLGVFTSRGIPLHKLFAQFQGKEM
                     GFTKGRDRSFHFGTKEHKVVGMISHLGPQLGIADGIALAHKLRKEERCTLVYSGDGGA
                     SEGDFHESINVAAVWDLPVIFVIENNQWGLSTPSNEQFRCKQFIDKAIGYGIEGIQVD
                     GNNILEVYDTTRRIAEDIRKNPRPVLLECITFRMRGHEEASGTKYYPEGLQEKWSVKD
                     PVDNFESWLISEKIISAADAEKSRMEIKKKIADELEIAFAEPNVEANTARELADVYAP
                     HNGAAIAPKNASKTEKRFIDAVSDGLRQAMQHHPNLVLMGQDIAEYGGVFKVTENFVA
                     EFGKDRVRNTPLCESAIIGTALGLSINGHKAMSEMQFADFVTCGFNQIVNNLAKIHYR
                     WGQNADVVVRMPTGAGVGAGPFHSQSNEAWFFHTPGLKVVYPAFPNEAKGLLLAAFAD
                     PNPVIFFEHKALYRSVSEEVNDDYFTLEIGKAAMVAEGNDVSIVTYGMGVHWALEVTK
                     ELGISADIVNLRTLLPLDTHTIYASVRKTGRVLVLHEDCMTGGIGGELSALITEHCFE
                     SLDAPVVRVASLDSPVPFAIPLENDFLPKDRFKEKLKSLLNY"
     gene            8009..8584
                     /locus_tag="PK610_02100"
     CDS             8009..8584
                     /locus_tag="PK610_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34744.1"
                     /translation="MKKRILPVIAVLALYACSGSPTTNNETQKKAMSFEEFAAQFPET
                     QPENTFFCSDSTYTAGSLKEIVAGDSAIVQLVPDILFQNGGYAWGSIHENDDFMMLIV
                     LLKADKLGPLFITYQKGKLLDYKIGFQNPGLDEGFRSEECCSFDADHYLNFTDITQNW
                     DPADSAKTFKSDTIIKSTAPLFFGTFNDIFQ"
     gene            complement(8688..10406)
                     /locus_tag="PK610_02105"
     CDS             complement(8688..10406)
                     /locus_tag="PK610_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34745.1"
                     /translation="MKKNQLIKMSLAALAGAFFIQGCTLVKDIEYKVNPNPVEMHGDT
                     VTLNINGKFVEKGLHKKAVVEVTPVIIGSDGQERAFKTETFQGPKAAGNGKVIPKEGG
                     SFSYTAKVPYTPNMEVSEIKVKILPKKGTKEKALITTDKIADGTIITPYLMMSDDKVI
                     FGKDKFVRVTSHTAEMVINYEKGRHEVRGSELKDKDYVDFMKWVAAALKNERIDIKNI
                     TIPAYASPEGEIAINSTLADDRARTAAEAIAKEAKKMKWEKAQDAKSSFYVLQGKGED
                     WDGFKTELEATNIADKELILRVLSMYSDVNKREQEIRNMAKTFQELEKKVLPKLRRSA
                     VTLNYDLTGKTDQELTEWSKSKPDSLTIEELLFAATLSNDLNEKLRIYNEVSRIYPND
                     WRGFNNAGAILFMQGKLNDANSLFDKAAGLASSESIIKNNLAACARQKGDRAKAEKLL
                     GEASGAGDEVSYNMGLLNIQKGKYGEAVSQMSKFKTFNLALAQLLNGNGSEATSTLDG
                     SQEKDAALSFYLRAIIAARANKIEEVKSNLTSAIQKDSALKAKAAKDREFLKFASDAS
                     FQAIVK"
BASE COUNT         3054 a         2395 c         2281 g         2926 t
ORIGIN      
        1 ttttgtttct cgctctttcg gaaagcggca tgaattcctc cactgttatt cccgtgaaca
       61 acatcggtat tgtggtggca tccgcacttg caggattatt gctttttaaa gaacggtatt
      121 cgttgttgaa taaagtcgga ttgatgctga gtattgccgc cattttaatg atctcattcg
      181 gataattgaa atggtgtaca gcttcaaaag catcgttgcg tcctgtgaag gactttacaa
      241 ggaaaaagga agtaaattcc atgcctatgc ctttcctgtt gccaatgaag aagagatcaa
      301 acaacatctg aacgaactcc gcaaaaaaca tttcgatgcg cgtcaccatt gttacgcctt
      361 tcgcttagga ccggacggaa cccactaccg cgccaacgac gatcaggaac catcgggcac
      421 agcaggaaaa ccgattcatg gtcagttgtt gtcatttggt cttaccaaca ttctgctcgt
      481 tgtagtgcgt tatttcggcg gaacgaaact cggtgcaagc ggactgatcc acgcgtataa
      541 atcggccgca gaagacgcct tgaagcaatg tgtggtagta gaacacgacg tgatgaaagc
      601 gattgagctc gaattccctt atgagcgtta caatgatgtg atgcgattcg taagagaaac
      661 ccattcgaaa atcatacaac agcaactgga agaaccttcg cgaattcagc tggagatccc
      721 tttaaaaaac gaacaaacac tgcatgatcg ctgtgagcgt tatcatgaaa ttaaatttat
      781 tgaagagcaa tgaacgattt tgaactgcta aaaaaaatgt gcagcatcca cgcaccaagc
      841 ggagatgaaa gcgcaatgac tgattttctg ttatcgtaca tcaaaaaaga atcgaaaaac
      901 tggaaacata aagtcacggt atattccggt cccgaatttc aggactgcat catcctggta
      961 tttggcaaac cacgcacaac gatttatgcc cacattgatt ccatcggtta tacggcgggc
     1021 tacgaaaaca acctgcttcg tattggagga ccacaacccg gcgaaggcga tcgtttggta
     1081 ggaaaagatg ccaagggaaa aatagaatgc gaactggtag tgattgatca cgaagacgga
     1141 ggaagaggat tgaattacct gttcaaacga aaaatagaac gcggaacatc gcttacattc
     1201 aaacccattt ggcgagagag caaagaatat atccaatgct gctatatgga caatcgtttg
     1261 ggcgtgtaca atgcattaaa agttgcagaa acactgaaag acggagccat cgtattttca
     1321 acctacgaag aacacagtgg cggaacggtt ggtgcactag cgcgttttct gcttgaaaaa
     1381 tacaaggtgc gtcaatcgct catctcggat attacctggg taacccacgg cgtaaaacac
     1441 ggaaaaggag tagccatctc catgcgcgat tcaggtattc cgcgacaaag ctatgtgcgc
     1501 cgcattatcg atctggccaa aaaaagtaaa atcccctttc aactcgaagt agaaagcgca
     1561 ggaggaagcg acggaactgc cattcagaaa agcgataatc tcatcgactg gtgtttcatt
     1621 ggcgccccgg aagacaacgt gcacaccccc cacgaaaaag tgcataaaaa agatattgcg
     1681 gcgatgataa gtttgtattc ttatttgatg aaaaaacttt gagttaatta ccaatttaca
     1741 attatacaat ttccaattgg gttcgtccac catttacaga ttacatttct gctttggagc
     1801 aaatgaaccg caaagatttt ttacgtgaag actaccttat tttgatttag aatttcaatc
     1861 aggattatag atgtctttta aattttttca aggaaaacaa tgcctcgctg cgcgatgcct
     1921 tgaaggcgcc ttcggcgcta cgcaaagggg taccattgta aatccgtcgt caataattgt
     1981 tgtttgcgta gatcataatt acccagcact aatccccatc ctaaatcgta atcgatcctg
     2041 aagcgaaatc gacgttatga gatttctgct tcagtaactg ttcactgttc acctttcact
     2101 gttcaccttt cacctttcac tgttcaccaa aaaaaaaccg cagcacttac aatttcacgg
     2161 atgaaactga aatgctgcgg aaaaaaaata gggtattgat taagcgatcg gctcgagaat
     2221 ttctacgagg cgaatatcaa agctcaggtc ttttccggct aaaggatgat tggcatcgat
     2281 ggtgatgtgt tccaatccta cttcacgtac tacaacaatc agctgatgac catcgggcgt
     2341 ttcggaaacc agttccattc ccacttccgg ttgcatatct tcaggtaagt gttcgcgggg
     2401 aactttctga atcatttcat ccattacagg accataggct tcatcagccg gaatattgat
     2461 cgttttgctc tcgttgagtg ccattccgat cacactgtcc tcaaatccgg gaatcagcat
     2521 accgcttcca attacgaatt caaggggttc gttgccttcc gacgaatcga agacaacacc
     2581 atcagacaaa cgtcccgtat aatgtacgcg aacgatgtcg ttttcttttg cttgtgccat
     2641 tataaaaatt taaaaaatta gagcgtccag tctaaacatc gctccgggtt actatgtaaa
     2701 aacgaaggaa ataaatactg ctaccccgca aaagtaggca attttcggag tagttgaccc
     2761 gaaaaggcca atacctgccg gattcgggtc ttcgttaccg atttagctgt taactttggc
     2821 agcaaacgaa aacacatggc aaaccccaag gcagaactca actttctgga cggaccccga
     2881 tcgcgctgga aggagctcgg attcgcaata cgcgtattcc tggaattcat taagggattc
     2941 agatcccttc acttttcagg gccttgtgtt accgttttcg gatctgcacg cttcaaggaa
     3001 ggacaccctt cgtacgaaat gacccgtgaa atttctgctg aaatggtcaa actcggattc
     3061 accatcctca ccggtggagg tccgggcatc atggaagccg ctaaccgagg tgcaaaagaa
     3121 gcaggtggaa gaagcattgg ttgcaatatt gaacttccat ttgaacaaca ccataatcct
     3181 tatctcgatc gttgggtgac gatgcgttat ttctttgtgc gaaaagtgct gcttaccaag
     3241 tattcctatg cctttgttgt aatgcctggc ggatttggaa cgctggatga attttttgaa
     3301 gcgatcacac tgatacaaac aaaaaaactg aaaccattcc cgatcgtaat tatgggagtg
     3361 gaataccaca aacacctcct cgaacacatc gatctgatga tcaaacacgg aacgatcagt
     3421 caggaggaca tgaaactgta ctgcgttacc gattcggtga gcgaagcagc aaaattcatt
     3481 gaggaacatt cggtaatcaa atacgatctg aaagacattg aacacccaaa accgaatccg
     3541 attctcggag aaaactgaag atgcggaaag gtgttgtttg tcaggggaat ttctgacctc
     3601 catcatcatt ttgcgactta gcctttcata gattagcact aattacgatg tatatgcgaa
     3661 atctgattct tcttctgatt gctcttattt cattgagaaa tgataccatc gcccagcaga
     3721 aaaaaccggt tgtcgaactg atcgaactgg ataatcttgt ggggtattat ggtcaggata
     3781 aaaaattcgt agtgctcttt gaacaggcac gcatcatcaa aaaatttgag catgtatggc
     3841 tggtcaccta cggctacgaa tttgatgcac aacacggaga atcgctgggc catgtgcgtt
     3901 cggatgatgt aaccgacacc catggaaaat tgatcaaccg acagacactc gtcaacgcag
     3961 aaggcattaa cattttacct ggtggtgccg atcattttcg ctattcagga aatgcattca
     4021 ttgccaaccg cggcaggaat tttatgctat tcgatgcgaa aggaaaaaaa ctgatcgaca
     4081 ataccgaagc cattctcact tttgcaggag gatacgctgc ctacaaacaa cagggaagct
     4141 ggagttttat tcatgtaagt ggcaagattg tggaacgcaa ttattccgag ttacgtccac
     4201 tgatccatga atgcggcgga tatattgttc gtgcaaaaaa ttattattca tttatcgata
     4261 cgctgctgca accggttatt gtgggtgaat tcacttcgat gcaatggatc agctacgatc
     4321 attatgtggt tgaaaaagca ggaaaatacg gtgtgatcaa ttcgcacgat aagtttctgt
     4381 tgccggtaga gtttgagcga ctcaatgcca atcaattcct caatgaagaa attgttattg
     4441 cacgccgcaa cggaaaagat cagttgctca gtccatggtt caagtttatc ggacgcccat
     4501 acgatcatat caaaaaatcg gaaaccggtt tttacgaagt gcgcgaaaac acatcttacg
     4561 gaatcatcga cagcaacgga cgcgaaattg tagctcccga atatgaggta tgcgatatga
     4621 tttccgaatg gggagtgcgt gtaaaaaaag gaatttatta cggactcatc tcctggcagg
     4681 gaaaaacaat tctccctttg aaatacacca ccttgggctt acctgccaac gaacggatcg
     4741 tattgggcat taccgatact ctttccggaa aaagcagttt tacctattgc gataaaacag
     4801 gagaactact cagcggattc aactacgaaa gcgcgcgatc attcagcgaa gggctcgcag
     4861 ctgtccgcaa aaacggaaaa ttcggtttta ttgataccac cggtaaactg gttgttacct
     4921 atcagtttgt agaagcatcc tcctttaaaa acggttattg cgtggtcgcc aaaaaagcag
     4981 gaaaatacgg tatgatcgac atcaccggat taatgatcat ttcttttaaa tacgaaaagc
     5041 tcgacgatct ttcggaggac atgattggct ttagaaaaaa cgggaaatgg ggattcctca
     5101 atgcaaacgg tgagcaggta atcgcacctc aatactccga aattctttcc ttttcagaag
     5161 gaatggcagc ggtgaaggat ggtccatact ggatctacat caataagaac aaggatctgc
     5221 ttatcgacat gcgcattgtc cgcgccgaat cattcagcga tggaaacgcc gttatacgca
     5281 tcgaaaacag cggaagattt ctgatcgatc gaaatgggca cattattcgg gaaatcaaac
     5341 cctgaagaga aacctcctta cgtcggtttc gcttcagggt tgagtttgtt ttggaattct
     5401 cgtcttcgtc ttccttttcg tcctcgtctt catcttcata ctcctacctg catccgtttt
     5461 caggagcgag agaataatca aagataagcg atgggcattc taccaattgt tcgctgccgc
     5521 cttttagatt gacgatgttg gttcctgatt cgtaatagat gacatctccc ccgccgcgtg
     5581 aatctatttt tgatcctgat ttcgcgtaga tcgtattgcc tccacccttc gatacaattt
     5641 cggcgccggg ttctagaaat ataatggaat aaccgccatt ctcacgtaat ttttttccgc
     5701 cacataccca aatgatgttt cttccctgat tggcaactgc attcttattg gcaacataag
     5761 ccattgcagg aatacgaaac gtacaaggtc cgatcgcaat tgtaccaatg agtccggact
     5821 gcgcagggac acgcttacgg caggatgtga tcgaaaacaa taccagtgca gaaaataaaa
     5881 aaatgaaaat ccttttacac tgcatagtaa aaaatcaata gttcaacagc gacttcaatt
     5941 tctctttaaa gcgatccttc ggaaggaaat cgttctctaa aggaattgca aatggtaccg
     6001 gagaatccaa tgaagcaaca cgcactacag gagcatcgag cgattcgaaa caatgttcgg
     6061 tgatcaatgc agaaagctct ccaccaattc cgccggtcat gcaatcttca tgcagcacca
     6121 acactcttcc tgttttgcgt acagatgcat aaatggtatg cgtatccaat ggaagaagcg
     6181 tgcgaagatt aacgatatcg gcactgatgc ctaattcttt tgttacctcc aatgcccaat
     6241 gcacacccat tccgtaagtg acaatactta catcatttcc ttctgcaacc attgcggctt
     6301 tccctatttc aagtgtgaaa taatcgtcgt tcacctcttc actcacgctg cggtaaagtg
     6361 ctttgtgctc aaagaaaatt actgggttgg gatcggcaaa tgcggcaagc aataatcctt
     6421 ttgcttcatt gggaaatgca ggatatacta cttttaatcc gggtgtatga aaaaaccagg
     6481 cttcattgct ctgcgagtgg aatggaccag cacctactcc tgctcctgtt ggcatacgca
     6541 caactacatc ggcattttgt ccccagcggt aatggatctt tgcaagatta tttacgatct
     6601 gattgaaacc gcaagtcaca aaatcggcaa actgcatttc actcatcgct ttgtgtccgt
     6661 taatggaaag tcccaatgct gttccgataa ttgcagattc gcataagggt gtattgcgca
     6721 cacgatcttt tccaaattca gcaacaaagt tttcagtcac cttaaacaca ccaccgtatt
     6781 cggcaatgtc ttgtcccatc aaaaccagat tgggatgatg ttgcatagcc tgacgcaaac
     6841 catcactcac tgcatcgata aaacgttttt ctgttttgga tgcgttttta ggtgcaatgg
     6901 cagctccatt gtgtggtgca taaacatcgg ccagttcacg tgccgtattt gcttccacat
     6961 tgggttctgc aaatgcaatt tccaactcat cggcgatctt tttcttgatc tccatgcgtg
     7021 atttttctgc atcggccgcg gaaattattt tttcgctgat gagccagctt tcaaaattat
     7081 ctacgggatc ttttaccgac catttctcct gcaatccttc aggataatat tttgttccgg
     7141 aagcttcttc gtgaccacgc atacggaatg taatgcactc gagtaatacg ggacgaggat
     7201 tcttgcggat atcttctgca atgcgtcgtg tagtatcgta cacttcgaga atattgtttc
     7261 cgtctacctg aataccttct attccgtaac cgatagcttt atcaataaat tgtttgcagc
     7321 ggaattgttc attggaagga gtggataatc cccactgatt gttttcgatc acaaagatga
     7381 ccggaagatc ccaaacagcg gcgacattga ttgactcgtg aaaatcgcct tcgcttgcac
     7441 cgccgtcgcc cgaataaacg agtgtgcaac gttcttcctt acgcaatttg tgcgcaagag
     7501 caattccatc tgcaataccc aattgcggac caagatgcga gatcattccc accaccttgt
     7561 gttctttcgt tccaaagtga aacgaacggt ctcgtccttt ggtgaatccc atttctttcc
     7621 cttgaaactg cgcgaacaat ttatgaagag gaataccacg cgaagtaaaa acaccaaggt
     7681 tacggtgcat gggaagaatg tattcttcct tggcgatagc cattgctgtc ccaacagaaa
     7741 ttccttcctg accatatcct gaaaaccatt tcgaaatacg accctgacgt agcaggatga
     7801 gcattttttc ttcgatcagt cgcggtaaga gaattgcatt gtaaagttca atcagctgat
     7861 cgttgctgta cgttttacga tcgaatggta acaccgggtt tgcggtagcg gacatggcag
     7921 taatttttca tggcaaaggt aaggcataat cagccactat cattagggga aatgggcagt
     7981 taggattaac ttgcatgaaa agcaccgtat gaaaaaaaga atcctaccag tgattgccgt
     8041 tttagcgctg tatgcctgtt ccggtagtcc caccacaaac aacgaaacgc aaaagaaagc
     8101 gatgagcttt gaggaattcg cagctcagtt ccccgaaaca cagccggaaa acaccttttt
     8161 ctgttccgac agcacttata ctgcaggcag cctgaaagag attgtagcgg gcgactcagc
     8221 cattgttcag ctggttccgg atatcctgtt tcaaaacggc ggttatgcct ggggaagcat
     8281 ccatgaaaac gatgatttta tgatgctgat cgttctgctg aaagccgata aacttggtcc
     8341 cctgttcatc acctaccaaa agggaaaact gttggactac aagatcggtt ttcaaaatcc
     8401 gggactggat gaaggtttcc gctctgaaga atgttgcagt ttcgatgccg atcattacct
     8461 caacttcacc gatattaccc aaaattggga tcctgctgat tcggcaaaga cgtttaaatc
     8521 ggacaccatt atcaaatcaa cagcaccttt attcttcggc accttcaatg atatttttca
     8581 ataatccgaa actgaaaaac ggatcctgaa gaaatacctc cttcatatta aagcataaaa
     8641 aaagccccgc atgtgcgggg cttttttatt tcaaagcatc gttatgctta cttcacgata
     8701 gcctggaaag aggcatcaga agcaaacttg aggaattcgc ggtctttagc agccttagcc
     8761 ttaagagcac tgtccttttg aatagcagat gtaaggttgc ttttcacttc ttcaatcttg
     8821 ttagcacgag cagcaatgat cgcacgcaag tagaatgaaa gagcagcatc tttttcctgt
     8881 gaaccgtcaa gggttgaagt agcttcagaa ccgttaccgt tcagcaattg tgcaagagcc
     8941 aggttaaagg tcttgaactt gctcatctgg ctaacagctt ctccgtactt acctttttgg
     9001 atgttcagaa gtcccatatt gtaagacact tcgtcacctg caccggaagc ttcgccaaga
     9061 agtttctcag cttttgcacg gtcacctttc tgacgagcac aagcagcgag gttgttcttg
     9121 atgatacttt cgcttgaagc aagacctgca gccttatcga acagactgtt ggcatcgttc
     9181 agtttaccct gcataaagag gatagcacct gcgttgttga aacctctcca gtcgttagga
     9241 tagatacgtg atacttcgtt gtagatacgg agcttctcgt taagatcgtt gctcaatgta
     9301 gcagcgaaaa gaagttcttc gatagtaagt gaatcaggct tagacttgct ccactcagta
     9361 agttcctggt cggtcttacc tgtaagatcg tagttcaatg ttacagcaga acgacgaagc
     9421 ttaggaagta ctttcttctc taattcctgg aacgtttttg ccatgttgcg gatttcctgc
     9481 tcacgcttgt taacgtcaga gtacatgctc aatacacgga ggataagttc cttatcagcg
     9541 atattggtag cctcaagttc ggttttgaaa ccgtcccagt cttcaccttt accttgaagt
     9601 acatagaatg aagatttagc atcctgtgct ttttcccact tcattttctt cgcttcctta
     9661 gcaatagctt cagcagctgt acgtgcacgg tcatcagcaa gtgtgctgtt gattgcgatt
     9721 tcaccttcgg gtgatgcata tgcaggaata gtgatgtttt tgatgtcgat acgctcgttc
     9781 ttaagagcag ctgcaaccca cttcataaaa tctacatagt ctttgtcttt caactcagaa
     9841 ccacgtactt catgacgacc tttttcgtag ttgatcacca tttctgcagt gtgcgaagta
     9901 acacgaacga acttgtcttt accgaaaatc actttgtcgt cgctcatcat gaggtacgga
     9961 gtgataatag ttccgtcagc gatcttatct gtggtgataa gcgccttttc ttttgttcct
    10021 tttttaggaa ggatcttaac cttgatctca gacacttcca tgtttggagt gtaaggaact
    10081 tttgcagtgt aagagaatga accaccttct ttagggatca ctttaccatt accggcagct
    10141 tttggtccct ggaacgtttc ggttttgaaa gctctctcct gaccatcaga tccgatgatt
    10201 actggagtaa cttccacaac agccttcttg tgaagacctt tttccacgaa cttaccgtta
    10261 atgttcaatg taacagtgtc accgtgcatt tccacgggat tagggtttac cttgtattcg
    10321 atgtctttaa caagggtaca accctgaatg aaaaacgcac ctgcgagagc agcaagcgac
    10381 attttgatca gttggttctt tttcatacga tgcttttgaa ttcgggtgca ataataacag
    10441 taaattgatt aaaaaaaaaa ccgagaggta ataaataggt ataaacttgt tgatttcgca
    10501 acccttggat ttaaagggat ttacgtgtaa tacctaccgg aaaatcagtg tttagtacct
    10561 tttcaatcaa caaaaaatcg tcctgagagc ctgcaaaatc aaggtttccg taatccaaaa
    10621 caaggattcg gaaatccctc agggtagcca gatgct
//
LOCUS       DAOMYG010000036        10514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2102_length_10514_cov_7.254613, whole genome shotgun sequence.
ACCESSION   DAOMYG010000036 DAOMYG010000000
VERSION     DAOMYG010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10514
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2102_length_10514_cov_7.254613"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1215)
                     /locus_tag="PK610_02110"
     CDS             complement(64..1215)
                     /locus_tag="PK610_02110"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ34746.1"
                     /translation="MQPVFIPRAFEADFLNSILPQKVLIILGARRVGKTQFIRRLLEE
                     KFTGDYMLFNGDDQSTIDAFSERKIENFRRLFGTRELIVIDEAQKIPDIGQKLKLIVD
                     EMAHIKVIATGSSLFDLSNKLGEPLVGREKTFYLFPLSQSELNFYEDPIQTKNKLEER
                     LIFGSYPELEQVSDWKDKAEYLNGIVNSYLLRDILEMDGIRKSDKLMDLLRLIALQIG
                     KEVHNDELANNLKGISRNTVDQYLDLLSKVFVIYRVGGYNRNLRKEITKSSKWYFYDN
                     GIRNAILRNFNRIDFRTDKGELWENYLMSERIKRNHYRRNFINSYFWRTYDQQEIDLV
                     EESGNSLNAFEFKWSAEAKVRVPGAWNKAYPEATFSVIHSGNYLPFITE"
     gene            complement(1596..2351)
                     /locus_tag="PK610_02115"
     CDS             complement(1596..2351)
                     /locus_tag="PK610_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34747.1"
                     /translation="MAFVRILFLLLILQLFNGGNAFAQGDTIRIKVKVTDADNPHIWM
                     NFFAVNKRSKQGTFGDRWSEFDMIVIKTDTITIKSNGYIPVLFSMKDSVSAPLKVFTV
                     KMHKEAILLPEATVVVVREFKEIEKDIKRLEMKKTSPYTGNYTKIASPITALYEAFSK
                     VEQEKRKASKLEYEDRKRELLKELLAKYVKGDIITLSESEFDDFIAFADPDMEFLQRA
                     SQYDLIMYFKTRFMEYDLYVRVKNLEPVKQQNK"
     gene            2445..3047
                     /locus_tag="PK610_02120"
     CDS             2445..3047
                     /locus_tag="PK610_02120"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRJ34748.1"
                     /translation="MPKRIPEVDRYIAKSSPFAQPIMNHLRDLVHQTCPEAVEVIKWG
                     FPNFEYKGNLCSMAAFKEHCTFGFWRGIELPDPEGLLERIGKTAMGNLGKLKKIEDLP
                     KQIHLEHLIREAMKLNESNSSAPKLKRPKTIISINLPSELNKAFSQKKNKDAQHFFES
                     LAPSHKKEFCKWINEAKKAETRIRRIEQTLIMLKEKKKKM"
     gene            complement(3049..3495)
                     /locus_tag="PK610_02125"
     CDS             complement(3049..3495)
                     /locus_tag="PK610_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34749.1"
                     /translation="MFTYEIPNWLIIVGAIGQMFTAVVYPYVRHVVFDWYNDIKKLKP
                     LNQEIAKTYGRYIQGLNFSFGLISILFTNDLKDQSGLAVALTGLIAAYWTGKVITQFS
                     YYPMYEIPNKPIIKIGELLMNTLFITFSLLFIWLFIYNITGYLATE"
     gene            complement(3553..4023)
                     /locus_tag="PK610_02130"
     CDS             complement(3553..4023)
                     /locus_tag="PK610_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34750.1"
                     /translation="MSIALCSNFATMSLGGDIEKRIIQDLTWIKGKFSLMRKNLSKEF
                     RKSKVVAGDLRHNAEQALTDLRTGKVFTDDHALENLRNTLGDSLKAAGMTGIFILPGG
                     SVGLIALRKMLKSKPAEKLGIENLLTLTVEESRKIEEEKKQEEEGNEQPKDEEE"
     gene            4127..5125
                     /locus_tag="PK610_02135"
     CDS             4127..5125
                     /locus_tag="PK610_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRJ34751.1"
                     /translation="MKIAYANYQLKFNHPFRLAHGVREGTSSVFVRLSFKGKSGYGEA
                     TLPPYLEETPESVINWLKDTDLDGLSPIRDFEEALQQIHRYSNDHSFVRCALEEALLD
                     LGGYSRYPIRSKVPCTFTLGLCENEQVLQDKLTSAAHFKFLKLKLNSDSDRTPVLQLR
                     KHSNLPFCVDANQAWSNVEKALDFTHWLKDQGAVLIEQPFGKDEMEKHAQFLESSPLP
                     SIADESIQNLDDLHERHTFFSGVNVKLLKCGGPLEAEAMLRFAKEKNLFRLIGCMSES
                     TLGVSHALSLSKYADMLDLDGPFLISNDPFKGYGIHKGKPFALQPELVNTLDFISL"
     gene            5122..5481
                     /locus_tag="PK610_02140"
     CDS             5122..5481
                     /locus_tag="PK610_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRJ34752.1"
                     /translation="MKTTLVIGASENTERYSNMAMKLLQEYGHPVIAVGNKAGMVNGI
                     PIETGTPHFENVDTVTLYLGPKNQVPVYPYILSLKPGRVIFNPGTENPELQKLLNEKG
                     IAWEEACTLVLLRTGGY"
     gene            complement(5482..5880)
                     /gene="msrB"
                     /locus_tag="PK610_02145"
     CDS             complement(5482..5880)
                     /gene="msrB"
                     /locus_tag="PK610_02145"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRJ34753.1"
                     /translation="MPFLVNKSDEEWKRELNPEEYRVLRLKGTERPFTGEYAMHFENG
                     NYSCRGCGEVLFSSASKFDAHCGWPSFDSELPGNKISKIRDTSHGMIRTEIVCANCGG
                     HLGHLFDDGPTNTGLRYCVNSVSIRFIKGE"
     gene            5969..6943
                     /locus_tag="PK610_02150"
     CDS             5969..6943
                     /locus_tag="PK610_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34754.1"
                     /translation="MSESTEHTPRHKRELTLWQLIPLIVIVLLMVFPLFIIIYNHVPP
                     FHFPGTDLRIDHVLTFIIVFLSVYYLARRIRHMLFLCFATGIFTMIVLSFIGRYSFGE
                     VIKDYRSFLYSINQSAIRFEFEEEVDPDDMIFPKAGEFRNAVDYKNPLVRNYAAEIAV
                     MHFERYATGNNLRVVQFFSIFKEIRKRWRYVFDPVDGDYFAKASETVMQLRSDGKFKG
                     DCDDYSILMGACIKAVGGEVRLVRTQVDVGGMSMGHVYPEVLVGNQKDLENINYLVKE
                     VLFVKESEGKPLFYHVDVNHKVWLNFDYNDHYPGGKYQSTIRIAELRI"
     gene            7006..7377
                     /locus_tag="PK610_02155"
     CDS             7006..7377
                     /locus_tag="PK610_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34755.1"
                     /translation="MPKIFIFIALLSIGFIACRKDKAVSPESCSLRTYSFSADVQPII
                     QANCAINACHGTNANAPFTLLSYGQVDTAVRFHNLLRAIKHEGNTPMPRIDPFQPAAN
                     KLPDSLIRIISCWVEQGRLNN"
     gene            7383..8453
                     /locus_tag="PK610_02160"
     CDS             7383..8453
                     /locus_tag="PK610_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRJ34756.1"
                     /translation="MIAKTFLTTTLIAGGIWYSGSYSTERPSARLDSNLPVAALLFEM
                     GEQKPDHFIAPIQPELALKGKEIIETGRTTDANGKKTSKQSRHFVCTNCHNTKREDPD
                     LSVSDPEARLKYVSAINLPLLPATTLHGVVNRESWYNDDYAKKYGALVLPARNNLKEA
                     IQVCATQCSQGRALDAFEMEAVLHYLWSIQLTIGDLNFRDHISEADTLAYFYQYAKTM
                     GNADKSNTEADESFRTSVKRKYLQASPAHFVSDQGPNGRSYGKNGNPVNGELIYEFAC
                     MSCHKNGGVTNFKFDKEKNTFRFLKRHMKRREFFSVYHHVLNGTYAKPGYRPYMPNYT
                     RERLSEQQLEDLVSYIHQQAEK"
     gene            8450..9337
                     /locus_tag="PK610_02165"
     CDS             8450..9337
                     /locus_tag="PK610_02165"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HRJ34757.1"
                     /translation="MKLLYTAFAIVVSGTLIAQQTKPDPPQKTSVRTFNSTRLINGHS
                     VETLWGRSLDVVISHRFGDMATPGAARTMFGLDNASDIRIALEYGISNTMNVGIGRSK
                     GAGPQTQLLDGYFKYNLLRQTEDNTMPVSMTFLGTSSFSMMQASTDSSSAVAFHKGEW
                     TQRLSYCTQLIIARKFSDRFSMQIVPSYIHRNFVAFDDKNGLMAIGAGARIKITKMFS
                     LVGEYHYLFDHNRVVNGTTYYDPVAVGFLFDTGGHTFQLNLTNSAGLGETQYLPHTYS
                     KLNEGEFRFGFTISRIVKF"
     gene            9340..>10514
                     /locus_tag="PK610_02170"
     CDS             9340..>10514
                     /locus_tag="PK610_02170"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ34758.1"
                     /translation="MKRILILFLLLSTNRSYAQAELDQLYANLENAKSENEKCDLYNE
                     IALIVVNSDPEQAREYATKALQSAEKANYKDGMGFAYYNYGNINYYLDEYEEGKKNLG
                     EAKKIFEGTGNEKGLGYVFNTSGEILTLEGAYGDALTNLFEALKHFEKIKDNVGLARV
                     NNNIGLIHYYQKNYEEALKYFNQALVTADETRVGDASLYIGRVFIEKNNYTEAETYLK
                     KALEIGVKNEDNYIISDCYYLLGRIDAFYGESEKAMDEFQKSMALKEQLEDGQGIALC
                     SIHIGNLYLNQKDAAKARNYFYRAITLAKEIGIREELKDAYLGLSNAYHYEQQFDSAF
                     FYLDLHNEVFNELLSEEASKKLAKLEASLESQRREAQIESERKLQEYTRKVIVYSGIA
                     "
BASE COUNT         3314 a         2128 c         2023 g         3049 t
ORIGIN      
        1 gaataaggat ttggggatag gaagataatg gaagtggatt catctttcca acttcaggaa
       61 ttcttattca gttataaatg gcaggtaatt accagagtga attactgaaa atgttgcttc
      121 aggataagct ttattccatg ctccaggtac tcttacttta gcttctgctg accatttaaa
      181 ttcaaatgcg ttcaacgaat taccggattc ttcaacaaga tctatttcct gttgatcata
      241 agttctccag aaataagaat tgatgaaatt tcttcgatag tgatttcttt tgattcgttc
      301 agacataaga taattttccc acaattctcc cttatcagtt ctaaagtcaa tacgattgaa
      361 atttcgtaaa atagcattac ggattccgtt atcataaaaa taccatttac tggattttgt
      421 tatttccttt cgcagatttc gattatatcc accaaccctg taaataacaa aaacctttga
      481 aagtagatca aggtattggt caactgtatt cctgctgatg cctttcaaat tattagccaa
      541 ctcatcatta tgcacttctt ttccaatttg aagcgcaatg agacgaagca aatccattaa
      601 tttatcagac ttcctgattc catccatttc aagaatatct cttaataaat aggaattgac
      661 aatcccattg agatattcgg ctttgtcttt ccaatcagaa acttgttcga gttccggata
      721 ggacccaaaa atcaaacgtt cttccaattt gttttttgtt tgaatcggat cctcataaaa
      781 attcaattcc gattgtgata aaggaaatag ataaaaagtt ttctcccttc cgaccaatgg
      841 ttcacccaat ttattactta ggtcaaataa agatgatcct gttgctatca ccttaatatg
      901 cgccatttca tcaacgatga gtttcaattt ttgtccgatg tccggaattt tctgtgcttc
      961 atcaatcaca attaattcac gggttccaaa aagtcggcgg aaattttcaa tttttcgttc
     1021 tgagaatgca tcaatggttg actgatcatc accattaaac aacatgtaat cacctgtaaa
     1081 cttttcctct aacagtcgtc taatgaattg cgttttcccg actcgtcttg ctcccaaaat
     1141 gatcaaaact ttttgtggca aaatagagtt caaaaaatct gcttcaaagg cacgcggtat
     1201 aaatacaggt tgcattgcaa aattattttg acgcaattta cataaataaa gtcatttaac
     1261 tgacgcaatt tacaaaaatt atgtcaaaaa attaacaaat ttataattct aacctgacaa
     1321 tctatcaaca aaaattctat agatttttga aaatcaacta tttaaaacaa aaaaatttca
     1381 ctctttattc aaaaacaaaa aattaaggat agtattatac actaaagcaa tagtaagtgg
     1441 ggagaaaaac actttttttg aatggaacat tcacatcaca aatttgcaat gcaaaacctt
     1501 tcttaggtta tctcataaaa gaaattgcaa aacaatagtc taccaagtat aagatgtgag
     1561 ttacaaaata ttaaccagcg gaattaaacc aacaactatt tattttgctg tttaaccggt
     1621 tccagatttt ttactcttac atacaaatca tactccataa atcgcgtctt gaaatacatg
     1681 atgagatcgt attgcgaagc gcgttgcaga aactccatat caggatcggc aaatgcaata
     1741 aaatcgtcaa actccgattc ggaaagggta atgatatctc ctttcacata tttcgcgagc
     1801 aactctttca acaattcacg tttgcggtct tcgtattcca gttttgaagc tttacgcttt
     1861 tcctgttcta ccttgctgaa tgcttcgtat aaggcagtaa tcggactggc aatcttagta
     1921 tagttaccgg tataaggtga agtttttttc atctccaggc gcttaatatc cttctcaatc
     1981 tccttgaact cacgcactac aacaacggta gcctccggaa gaagtatggc ttctttatgc
     2041 atcttaaccg taaatacctt taatggtgct gaaacagaat ccttcattga aaatagtacg
     2101 ggaatatatc cgtttgactt aatggtgatc gtatctgttt tgatgacaat catatcgaat
     2161 tccgaccatc tatcgccgaa tgttccttgc ttggagcgtt tattgaccgc aaaaaaattc
     2221 atccagatat gtggattatc cgcgtctgtc actttcactt ttatgcgaat cgtgtcacct
     2281 tgggcaaaag cattcccgcc attgaacagc tgaaggataa gcagtaagaa caaaatgcgg
     2341 acaaatgcca taatggaaag gtaaagaatt tataaaatca aactgttttt gaccaggatt
     2401 cgatttcaaa atttaattat tttaacatac gtaaagccaa aatcatgcct aaaagaatac
     2461 ctgaagttga ccgatacatt gccaaatctt caccctttgc acaaccgata atgaatcact
     2521 tacgggatct ggttcaccaa acttgtcctg aagctgttga agtgataaaa tggggattcc
     2581 ccaatttcga atacaaggga aatttatgca gtatggccgc atttaaggag cattgtacgt
     2641 ttggattctg gagaggaatt gaactacctg atccggaagg attgcttgag aggattggaa
     2701 aaactgccat gggaaatctg ggaaaactca aaaaaataga ggatctgccc aagcaaatac
     2761 atctcgaaca tctgattcgg gaggccatga aattgaatga aagcaattca agtgccccca
     2821 aattgaaacg tccaaaaacg attatttcga ttaacctgcc gtctgaattg aataaagctt
     2881 tttcacaaaa aaagaacaaa gacgcacaac actttttcga aagtttagcg ccttctcata
     2941 agaaagaatt ctgcaaatgg attaatgaag caaaaaaggc agaaacccgt attcgcagaa
     3001 ttgaacaaac ccttatcatg ctaaaggaaa aaaagaaaaa aatgtgattt attcggtagc
     3061 aaggtatccg gtgatattat atataaaaag ccagataaaa agcaatgaaa aggtaatgaa
     3121 caaggtattc atcaacagtt ctccgatttt gataattggc ttattgggta tttcatacat
     3181 cggatagtaa gaaaactgag tgattacctt tcctgtccaa tatgcagcaa ttaatccggt
     3241 tagcgccact gccaaacctg attggtcttt cagatcattg gtaaacaaaa ttgatatcaa
     3301 tccaaacgaa aagttcaaac cctgaatgta cctaccatag gtttttgcaa tctcctgatt
     3361 cagtggtttt agctttttaa tatcgttgta ccaatcaaaa actacatgtc gtacgtaagg
     3421 atacaccaca gcagtaaaca tttgtccgat tgctccaaca atgattaacc aattgggtat
     3481 ttcataggta aacattcctt ctaaatctaa aacaatacaa cagttgaaaa acactttatc
     3541 ctgaaaggac gattattctt cttcatcctt tggttgctca tttccttctt cttcctgctt
     3601 tttttcttct tcaattttcc ggctttcttc aacggtaagc gtaagcagat tttcgattcc
     3661 taatttttca gccggtttgc ttttaagcat cttacgcaaa gcaatcaatc cgacagaacc
     3721 tccgggaaga ataaatatcc ctgtcatgcc tgcagctttc agcgaatcgc ccagtgtgtt
     3781 gcgtaaattc tcaagcgcat gatcgtctgt aaacaccttt ccagtccgaa gatctgtcag
     3841 ggcctgttcg gcattatggc gcaaatcgcc ggcaacaact ttgcttttac gaaattcctt
     3901 actcaggttt tttcgcatta aactgaattt tccttttatc caggttagat cctgaataat
     3961 ccgcttttcg atatctccac caaggctcat cgttgcaaaa ttagagcata aagcaataga
     4021 caaaactgag atttgccgac ttttctcttt tttttaagcc acatccggtt aacaacctcg
     4081 gtctaaattc cgcctaaaca aagctcaact cttttatttt taccctgtga aaattgccta
     4141 cgcaaactat caactcaaat tcaatcatcc tttccgcctg gcacacggtg taagggaagg
     4201 gacttcaagt gtctttgtac gactgagttt caagggaaaa agcggttacg gtgaggctac
     4261 actcccaccc tatcttgaag aaactccgga aagcgtaatc aactggttga aagatacaga
     4321 ccttgacggg cttagcccca ttagggattt tgaagaggct ttgcaacaaa ttcaccgtta
     4381 ctccaacgat cactcttttg tacgatgtgc attggaagaa gcattactcg atcttggggg
     4441 atattcccgt taccccatcc gatcgaaagt tccctgtacg tttaccttgg gtttgtgcga
     4501 aaatgaacag gtacttcagg ataaacttac aagtgcagcg catttcaaat tcctcaaatt
     4561 gaaattgaat tcagattcgg atcgaacacc tgtgctgcaa ctcagaaaac acagcaatct
     4621 tccattttgt gtggatgcga atcaggcctg gtcaaatgtg gaaaaggcgc ttgactttac
     4681 gcattggtta aaagatcagg gtgctgtact tatcgagcag ccttttggca aggatgaaat
     4741 ggaaaaacac gcacaatttc ttgagtcgag tccattgcct tccattgctg atgaatccat
     4801 ccaaaatctg gatgatcttc acgaaagaca tacatttttc tcgggtgtta acgttaaatt
     4861 actgaaatgc ggaggcccac tagaggcgga ggcgatgctt cgttttgcta aggaaaaaaa
     4921 tctgtttcgt ctgatcgggt gtatgtcaga atcaacattg ggagtgagtc atgctctctc
     4981 actttcaaaa tatgcagata tgctagatct cgacggtcct tttctaatca gtaatgatcc
     5041 gtttaaaggt tatggtattc ataagggaaa accatttgcc ctgcagcctg aacttgtgaa
     5101 tacacttgat tttatctcat tatgaaaacg acactcgtta ttggtgccag tgaaaacacc
     5161 gaaaggtatt cgaatatggc aatgaaacta ttgcaggaat atggtcaccc tgtaattgca
     5221 gttggcaaca aggcaggaat ggtgaacgga attccaatcg aaaccggaac accccatttc
     5281 gaaaatgtgg atactgtaac attgtatctc ggtccaaaaa atcaggtccc tgtttaccct
     5341 tacattcttt ccttaaaacc cggtcgtgtt attttcaatc ccggcacaga aaatccagag
     5401 cttcagaaat tgcttaatga aaaaggtatt gcatgggaag aggcctgcac tctggtattg
     5461 cttcgcactg gaggatatta actactctcc cttaatgaat cggatagaaa ccgaatttac
     5521 acaataacgc agaccggtgt tggttggacc atcatcgaac aaatgaccaa ggtgtccacc
     5581 gcaatttgca caaacaattt cagtccgtat cataccatgt gaagtgtcgc gaatcttgga
     5641 tattttatta cctggcaatt cactgtcaaa actgggccat ccgcagtggg catcaaattt
     5701 ggaagcagat gaaaacaaca cttcaccaca accgcgacag gaataatttc cattttcgaa
     5761 atgcatcgca tattctccgg tgaaaggacg ctcagtgcct ttgaggcgaa gcacccggta
     5821 ttcctcagga tttaactccc gtttccattc ttcatccgac ttatttacaa gaaaaggcat
     5881 accgctgaat aaaagattcg tgaataagat aacgaaataa acagtttttt cgttcttaag
     5941 ctaacacgca ttgcctattt ttgttcgaat gagcgaaagc acagaacata ccccacgcca
     6001 taagagagaa ttgaccctat ggcagttgat accgctcatt gtgatcgtgc tactgatggt
     6061 gtttccttta ttcatcatca tttacaatca tgttccgccc tttcattttc cgggaaccga
     6121 tttgaggatt gatcatgtgt tgactttcat aattgtgttt ctcagcgtat attatctcgc
     6181 aagaagaatc cgacacatgc tttttctttg cttcgctaca ggaatattca ccatgattgt
     6241 tctatccttt ataggcaggt attccttcgg agaagtaatt aaggattacc gttcattcct
     6301 ctacagtatc aatcaaagtg ctattcggtt cgaatttgaa gaagaagttg atccggatga
     6361 tatgattttt ccgaaagcag gtgaattcag aaatgcagtt gattataaaa atccattggt
     6421 gcgcaattat gctgcagaaa ttgcggtaat gcactttgaa cgctatgcaa caggaaacaa
     6481 tcttcgagtt gtgcagttct tctctatttt caaagagatc cgtaaacgat ggaggtatgt
     6541 ttttgatcca gttgatggtg attatttcgc aaaagcttca gaaacggtaa tgcaattacg
     6601 cagcgacggg aaatttaaag gcgattgtga tgattattcc atcctgatgg gagcttgcat
     6661 taaagctgtt ggaggagaag tcaggttggt ccgcacacaa gtagatgtgg gtgggatgtc
     6721 gatggggcat gtttatcccg aagtattggt tggcaatcag aaggatctcg aaaacatcaa
     6781 ttatctggtt aaggaagtcc tgtttgtaaa agaaagcgaa gggaaaccat tgttttatca
     6841 tgtcgatgta aaccataaag tctggctcaa tttcgattac aatgatcatt atccgggggg
     6901 aaagtatcaa agcacgattc gcattgcaga gctaaggata taatccacac gccttcgttt
     6961 catggattta cagattcttt atatattgga tcaaaatccg tcacgatgcc gaaaatattc
     7021 attttcattg ctttactctc tattggtttt attgcctgca gaaaggataa agcagtttcg
     7081 cctgaaagtt gctcccttcg cacctatagc ttttccgccg atgttcaacc catcattcag
     7141 gccaattgtg ctattaatgc ctgtcacggt acaaatgcca atgcgccatt tacgctgcta
     7201 agctatggtc aggtcgatac cgctgttcgc ttccataatt tgctacgtgc catcaaacac
     7261 gaaggaaata ctccaatgcc aaggatcgat ccgtttcaac ctgcggcgaa taaattaccc
     7321 gatagcctga tacgcatcat tagctgctgg gtagaacaag gtcgactcaa caattgaccc
     7381 ttatgattgc aaaaactttt cttacaacaa cgcttattgc aggaggcatt tggtacagtg
     7441 gttcctattc aactgaacgg ccatcagcac ggttggatag taatttaccg gttgctgcat
     7501 tattgttcga aatgggagaa caaaaacccg atcattttat cgctccgatt caaccggaac
     7561 tcgcgctgaa aggcaaagag atcattgaga ccggaaggac aaccgatgct aatggaaaaa
     7621 aaacaagcaa gcaatcgcgc cattttgtgt gcaccaattg tcataacaca aaacgcgaag
     7681 atcccgatct cagcgtctct gatccggaag cacgcctcaa atatgttagc gctatcaatc
     7741 taccgctttt acctgccact accttgcatg gagttgtaaa cagagagtcg tggtacaacg
     7801 atgattacgc aaaaaaatac ggtgctcttg ttttacctgc ccgcaataat ttaaaggaag
     7861 ctattcaggt ttgtgcaact caatgtagtc agggcagagc attggatgca tttgaaatgg
     7921 aagctgtgtt gcattacctg tggtcgattc agctgaccat tggcgacctg aattttcgtg
     7981 atcacatatc cgaagcggat acactggcct atttttatca gtatgcaaaa acaatgggga
     8041 atgccgataa atcaaatacc gaggctgatg aatcgttcag aacatcagta aaaagaaagt
     8101 acctgcaggc atcaccggcg cacttcgttt cagatcaggg tccgaacgga cgctcctacg
     8161 gaaaaaacgg caatccggta aatggagaac tcatttatga attcgcatgt atgagttgtc
     8221 acaaaaacgg tggagtaaca aatttcaagt ttgacaaaga gaagaataca tttcgctttt
     8281 taaaacgaca tatgaagcgg cgagaatttt tctcggtcta tcatcatgtt ttgaacggga
     8341 cctatgcaaa acccggttat cgtccgtata tgcccaatta cacccgcgag cgattgagtg
     8401 aacaacagct cgaggatctt gtatcttata ttcatcaaca agcagaaaaa tgaaattact
     8461 gtataccgca tttgcaatag ttgtatcagg aacattaatt gcacagcaga caaaacctga
     8521 tccaccgcaa aaaaccagtg tgcgtacatt taacagtaca cgactaatca atggacacag
     8581 tgtagaaacc ctgtggggac gatcgctgga tgtagtaatt tcccaccgat ttggagatat
     8641 ggcaaccccg ggagctgcac gcaccatgtt tggtctcgat aatgcatcgg acatccggat
     8701 tgcgcttgaa tatggaattt ccaatacaat gaatgttggt attggaagaa gcaaaggagc
     8761 aggaccgcaa acccaactgt tggacggcta tttcaaatac aatttgcttc gacagaccga
     8821 agataatacc atgccagtat ccatgacttt tttggggaca agttcattca gtatgatgca
     8881 ggcatccacc gattctagtt cggcggttgc atttcataag ggagaatgga cacaacgtct
     8941 ttcgtattgc actcaactga ttatagccag aaaattctct gatcgctttt ctatgcaaat
     9001 tgtaccctcc tacatacaca ggaattttgt tgcatttgat gataaaaacg gattaatggc
     9061 aattggtgca ggagcgcgga tcaagatcac taaaatgttc tcgctggtag gagaatacca
     9121 ttatttattc gaccataacc gagtagtaaa tggtaccact tattacgatc cggtggctgt
     9181 tggatttttg ttcgataccg gaggtcatac atttcagctc aatcttacca actctgcagg
     9241 tttaggtgaa acacaatatc tgccacatac ttattcgaaa ctgaatgaag gtgaattccg
     9301 cttcgggttt accatttcaa gaattgtaaa attctaatca tgaaaagaat tctgatttta
     9361 tttcttctgt tatctacaaa ccggtcctat gcacaagctg aattggatca attgtacgct
     9421 aatcttgaaa atgcaaaatc agaaaatgaa aaatgtgatc tctacaatga aattgcatta
     9481 atcgttgtaa attctgaccc cgaacaggct cgtgaatatg ctacaaaagc actgcaatcc
     9541 gccgaaaaag ccaactacaa agacggaatg ggttttgcct attataatta tggaaacatc
     9601 aattattacc tagacgaata tgaagaaggt aagaagaatc tgggcgaagc aaaaaaaata
     9661 tttgaaggaa ccggcaatga aaaaggatta gggtatgtat ttaataccag cggggagatc
     9721 ctcacgcttg aaggagcata tggcgatgcc ttaactaatt tatttgaagc gctgaaacac
     9781 tttgaaaaga tcaaagacaa cgttggcttg gcaagagtga acaataacat tgggttgatt
     9841 cactattacc agaagaatta tgaagaagca ttaaaatact ttaatcaggc acttgttaca
     9901 gccgatgaaa cccgcgttgg cgatgcttca ttgtatattg gtagggtttt cattgaaaaa
     9961 aacaattaca ccgaagcaga gacctacctc aaaaaagcac tggagatagg cgttaaaaac
    10021 gaagacaatt acatcatttc cgattgctat tacttattgg gaaggattga tgcgttttac
    10081 ggtgaatccg aaaaagcgat ggacgaattt caaaaatcca tggcacttaa agagcaattg
    10141 gaggatggtc agggaattgc actttgctct attcatatcg gaaatctcta tctcaatcag
    10201 aaagacgctg caaaagccag gaactatttc tacagagcaa ttactctggc aaaagaaatt
    10261 ggcattcggg aagaattgaa agatgcttat ttgggtttga gtaatgccta ccattacgaa
    10321 cagcaattcg acagtgcatt cttttatttg gatctgcata atgaagtttt caatgaatta
    10381 ttaagtgagg aagcttcaaa aaaacttgcc aaacttgaag catccctcga atcacagcgt
    10441 cgtgaagcac aaattgaatc tgaaagaaaa ctacaggagt ataccagaaa agtgattgtt
    10501 tattccggta tagc
//
LOCUS       DAOMYG010000037        10479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2112_length_10479_cov_11.974386, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000037 DAOMYG010000000
VERSION     DAOMYG010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10479
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2112_length_10479_cov_11.974386"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..7756
                     /locus_tag="PK610_02175"
     CDS             59..7756
                     /locus_tag="PK610_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34759.1"
                     /translation="MVVSDNSKIAHVGVVDVPYQKEIRPLKQELVLVNNGGTEQLKIR
                     NLFDQEVEDTDAIIAQILKEKGLLNPNSNEMIIDSLDKAREKKTEKVEKDPQLGNLSD
                     KDLIEMVKKDVEELVNESKDLKEKSEAAYVVADRKNDEARQKTKEAEEMLAKLNNVRD
                     ENERKQIIAEANDLNAEAKKLVNESTAAYNVAVSLDQQQKKKQDEAKVASEFAKGVES
                     AITSKNRKEAEKKLREQQEYIRSVMASESNRKDEIDELREKGRTKKLEADKALAIVKD
                     LRNEESQIARELNNLKKQQEQAKGDKKKQEFQREIDSKTEDLNAIQADIKTAQRKYES
                     LQLETDQIASQVSILEDIQSGNVKGSLTEAEKASLAREINSGESSLTIAKNEQVLQQN
                     AEINRDDQEKLNKYLYENPQYKGLLSKAEELELQSESTTKLEEKNRLNREWISTIDKD
                     VASLNDKLKKEKDPAKREELQIRIQSLENLKSNKELEIKRNENRIGELKGITTTLQPT
                     TGNTAAKVDPAFDQKRNEIKNSGLSPKEQLQRENELNRDLVTKTDERIAANNAKMNSV
                     DPMERERLEKENRELRDLKESTQNEISLNQRKIESGSSTVNPTRVTEPNKVDPNYDVS
                     LNKIKSSQKSPEDKLKEENKLNATLIAKVDKQIETLDKKIAKANGDEKEKLLQEKEGL
                     TNLKIQKQTEIRNNDQQIAAIKDPNKTIKSSNEITTAVDPNYSNKLNSIRNSNSSTQD
                     KLRSENDLNREFLQSAESRKAALEEQITGGTPEEKIAAQNEMSGLDKLIAEKRNDITT
                     NERRIQSLENTASTDPGVVKSTELASNTTLDKIDPSYDKQLASIEGSNLTSTQKAEER
                     KQLNEQTLNKVKEEKRRAEQAGNTEKAAELEKLESQLEAMVRKDESTMQGSRTPNVIQ
                     EEKNEKIAETVDPSYDAKVDAIQNSNKSEVRKTAETREANEELIRKTAEEISRLERNL
                     STGNVSDPEATRQRIEDLKDLRDEKEREVAQADKQLAEAGITRVDENGRSVSGYAFMP
                     EYSSAEAQKRIDALQPLIDKVRDIEDQLTELKIRRAESNNAAESDRITQEIIKLEAEL
                     VKLELEIASKVQEANQLEKKEIEAKVSTAKSEAKKVKVPADDPEFLTAQKLEEKAKTK
                     FEDAKKLRADAAKEKDEKARNLLLMKANVAEQLGNKYLNNAATSYSKSAKTAQGIDPE
                     QNIVKVSDIYPEYFTALETSSSSGDRSKQTEERIRLNEELIRRIDAQMSELKSTSTSD
                     PATANKLNQLGELKKQKEEELKRDKQTLEQLSERPELTSEKKNLIQTVDPGYVQSMER
                     IENSTSSEARKISERNDLNETLIEKLDKQIAESKAIIASTDDEKIKQDEERRLSELEN
                     LRMEKTRELSAGRIRLDKMGIASLPAQQQYENKYPYTSEKAQQEMQGIESQLTAIEAK
                     RKQIEDLKIRRYSSTDKAEQKKLDNEIKKAESELLTMEMNVAASFMSANAAEREATEK
                     RISEKKKETGYKDIPANHQVMKNVTALEQQAERIFSEAAELRRNANAEKDKNKRNELF
                     RQAHAKEREANSLLAEAEQQLDVLLGDKTLASANQKQNTGNNSGQEVAINTQQGGTTQ
                     QGGTTQQGGTTQQGGTTQQGGTTQQGGTTQQGGTTQQGGTTQQGGISSAQMADPANRE
                     STRQMAEADRLRVQGNNLIARAQQLEDSAKTVKKKNRQAVLDQSAQKRKEGEELLAQS
                     DRMNTKANETRRQEEEQIAEQRRKEEEMERAIAIADDPEYARYMQLREEARRAEVSAT
                     NAERDYQQRLNATADLRKRAQDTRAQASTETDNQRKNRLLTQASDLEQQAIRAERNYD
                     TTRNRATQRRTEANRAQTNADNYLAGMDPRKAQTIRDLASAGKLPDPKGTSLATNTTG
                     GTATTNGNQRTVTNAGTTSVVSPITTMDPEFVARKYSVPEKVETEIYVLSEKAVYNNA
                     NPIPVNPEMPKGIYFKVQVGAFRNQIPQETFKEFAPITAETTPQGLTRYTVGYFTTFN
                     VANGAKMEIRGMGYTDAFVVGFRDGKRIPLNEALEALGQSGQLATNTQQGGTTQQGGT
                     TQQGGTTQQGGTTQQGGTTQQGGTTQQGGTTQQGGTTQEFVNNFNPDRNATNYYNDPN
                     AAKANQVEVIPGLFFTVQVGVYSRPVSADKIFNLNPLNSEKTANDQIRYTTGVFTDIN
                     IATSRRDEIRQIGVTDAFVTAYLNGKRITIGQARDSIAKYGNSIFVNDQKLVSNTTTT
                     QQGGTTQQGGTTQQGGTTQQGGTTQQGGTTQQGGTTQTQDRQNVPQASSYKVILGQYK
                     GEVPSKEAEIFLTNMNYGIRKANVGDGSTVYFIDNVSSRTEAFNIADHFIQKGLPGAY
                     VSDKLPAQTTATKVEEPQAASTVRFQVYIGEFIGEVPPEATEIFLNNYDKGVKRKKLN
                     DGTIVYYVLDGSSYDEALKMKAHFISEGLSSVRIVAFDGSNEIPLSKAYTAGMRYRIV
                     VAEFAGEMPEEVKEAYGNVPELNVRTKAIDGGFTMAYADGITDIDQALRLKARLIAEG
                     LSTVRIVVVKDGKEISLSEAFRLVYEK"
     gene            7746..8045
                     /locus_tag="PK610_02180"
     CDS             7746..8045
                     /locus_tag="PK610_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HRJ34760.1"
                     /translation="MKNNFRLSTQPRWKEEEDVVVLEQDVDLRSIILFNDNVNTFGYV
                     IEMLIRYCKHEAVQAEQCTYLVHYTGKCEVKRGSYDELEPVCTQLLEAGLTAEIQ"
     gene            8048..8827
                     /locus_tag="PK610_02185"
     CDS             8048..8827
                     /locus_tag="PK610_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRJ34761.1"
                     /translation="MKQFFLAVLLTSVIVSCTRVPVTNRKQFRLLQEEQLILMADTAY
                     RDFLATAKVLPHNDPRTATVKRVGGKIQSSVIDYLTRKNALGRIRGFTWEFNVVDDPT
                     INAWCMPGGKVVVYTGILNLATDDTLLAVIMGHEIAHAIARHGNERMSQQMAIQGVGR
                     ALGGAMSDPVKRNIFLQSYGIGSALGILSYSRKHETESDKMGLVFMYLAGYNPSKAID
                     FWKKMAANGAAVPEFLSTHPSDETRIKDITDFLPLIPKYAQ"
     gene            8883..9239
                     /gene="trxA"
                     /locus_tag="PK610_02190"
     CDS             8883..9239
                     /gene="trxA"
                     /locus_tag="PK610_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRJ34762.1"
                     /translation="MPTVKLTAEKFKSDIFDYTTEKEWKYKGNVPAIIDFYADWCGPC
                     KMVAPLLEELSDEYTDKLIIYKVDTEVEQELSAVFGIRSIPSLLFIPREAQPMMQAGA
                     LPKAAFKEIIEEELLK"
     gene            9362..9991
                     /locus_tag="PK610_02195"
     CDS             9362..9991
                     /locus_tag="PK610_02195"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HRJ34763.1"
                     /translation="MNLTGIISISGKPGLYKVVSQSKNSLIVEGLTDKKRFPAFSADK
                     VSALDDISMYTTEEDVPLAEIIRKIWDKEKGGRCVDAKEEANAHHKYFAEILPNYDRE
                     RVYNSDLKKLFSWYNLLQETGTLKNLIEEAEKAAAAEKEEAKPKKKAAKEEKATEEKA
                     EVKESKAKEPKAKKPAAEKKEVKKTAAPKVKAESKAKTTVRTASMKRGA"
     gene            9995..>10479
                     /locus_tag="PK610_02200"
     CDS             9995..>10479
                     /locus_tag="PK610_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HRJ34764.1"
                     /translation="MASTGTDLPVRPPRRFIAQDLQIDAWSKLEPYFSTLLNRDLLSI
                     GDLEQWLQDRSELEAVVEEEGAWRYIRMTINTKDDKAVEAYEFFVTEIEPRIAPYADK
                     LNRKMMDCSFVNKLEKPGYSIYRRAIAQELKLFRDENVPLIAQIQKESQRYGAITGAQ
                     SV"
BASE COUNT         3583 a         2262 c         2479 g         2155 t
ORIGIN      
        1 acgttcaagc gatggcagct acctgattac cttaccgcgt tcggggaaat acaaatttgt
       61 ggttgttagc gataattcga agatcgctca tgttggtgtg gtggatgttc cttatcagaa
      121 agagattcgt ccgctcaaac aagaattggt attggtcaac aacggcggaa ccgaacaatt
      181 aaaaatccgg aatcttttcg atcaggaagt ggaagatacc gatgcaatca ttgcccagat
      241 cctaaaggaa aaaggattgc tcaatcccaa ttccaatgaa atgattatcg atagtctcga
      301 caaagcccgt gagaaaaaaa cggagaaagt cgagaaagat ccgcaattgg gtaacctgtc
      361 cgacaaagac cttatcgaaa tggtgaagaa ggatgtggag gaactggtga atgaatccaa
      421 agatcttaaa gagaaatcgg aagcagccta tgttgttgcc gatcgcaaaa atgatgaagc
      481 gcgacagaag accaaggaag ctgaagagat gcttgccaaa ctgaataacg tacgcgacga
      541 aaacgaacga aagcagatca ttgcagaagc caatgatttg aatgcagaag caaaaaagct
      601 ggtcaatgaa tctacggctg cctataatgt ggctgtaagt ctggaccaac aacaaaagaa
      661 gaaacaggac gaagcaaaag tagcttctga atttgcgaaa ggagtggagt ctgcgattac
      721 ctccaagaac agaaaagaag cagagaaaaa acttcgtgaa cagcaggaat atattcgttc
      781 agtaatggcc agcgaatcga atcgtaagga cgagatcgat gaattgcgcg aaaaaggaag
      841 aactaaaaag ctggaagccg acaaagcact tgcaattgtg aaagacctgc gtaacgaaga
      901 aagtcagatc gcacgtgagc tgaacaatct taaaaagcag caggagcagg caaaaggcga
      961 taaaaagaaa caggaatttc agcgcgagat tgatagcaaa acagaagatc tcaatgcgat
     1021 tcaggcagat attaaaacag cacagcgaaa atacgagagt ctgcaactgg aaaccgatca
     1081 gatcgcttca caggtttcca ttcttgagga tatccagtca ggaaacgtaa aaggatcact
     1141 taccgaagca gagaaggcat cattggcgcg tgaaatcaat tccggagaat catcacttac
     1201 cattgcaaag aatgaacagg ttctgcaaca gaatgcagag attaatcgcg acgatcagga
     1261 aaagctgaac aaataccttt atgaaaatcc gcaatacaaa ggactcttat caaaagcaga
     1321 agaactcgag ttgcaatcgg aaagcaccac caaacttgag gagaaaaacc ggttgaaccg
     1381 ggaatggatc agcaccatcg ataaagatgt ggcatcgctc aacgacaaac tcaaaaagga
     1441 aaaagatcct gccaagcgtg aggaattgca aatacgcatt cagtcccttg agaatttgaa
     1501 atcgaacaaa gaactcgaga tcaaacgcaa cgaaaacagg attggagaac taaaaggcat
     1561 cacaaccact ttacaaccta caaccggaaa tactgcagcc aaggttgatc ctgccttcga
     1621 tcagaaacgg aatgagataa aaaattcggg acttagtcca aaagagcaat tgcaacgcga
     1681 aaacgagttg aatcgtgatc tggttaccaa gaccgatgaa cgtatcgcag ccaacaatgc
     1741 aaaaatgaat tccgtcgatc cgatggaacg tgaacgtctg gaaaaagaga acagggaatt
     1801 acgtgatctg aaggaatcca ctcaaaacga aatcagcctc aatcagcgaa aaatagaaag
     1861 cggttcgtct acggtcaatc caactcgtgt aacagaacct aacaaagttg atccgaatta
     1921 cgatgtatcc ctgaataaaa tcaagtcctc acaaaaatct cctgaagaca aactcaaaga
     1981 ggaaaataaa ctgaatgcga cgctgatagc aaaagtggat aagcagatcg aaacgctgga
     2041 taaaaagata gcgaaggcaa acggtgacga aaaggaaaaa ctcctgcagg aaaaagaagg
     2101 cttgaccaat ctgaaaattc agaaacaaac cgaaatcagg aacaatgatc agcagattgc
     2161 tgcaataaaa gatccgaata aaaccattaa aagttccaac gaaatcacca cagctgttga
     2221 tcccaactac agcaacaagc tgaacagtat tcgtaattcc aattcaagca cgcaggataa
     2281 gctccgttcg gaaaacgacc tgaacaggga attcctgcaa tcggcagaat cgcgcaaggc
     2341 tgcattggaa gaacaaatta caggcggaac tcccgaagaa aaaattgccg cacaaaatga
     2401 aatgagtggt ctggataaac ttattgctga aaaacgcaac gatatcacta ccaacgagcg
     2461 ccgtattcaa agtctggaaa acaccgcctc caccgatcct ggagttgtaa aatcgacgga
     2521 gctggcaagc aatactactt tggataaaat tgatccctcg tacgataaac agcttgcgtc
     2581 catcgaaggc agtaatctta cttccacgca aaaagctgaa gagcgaaagc aattgaatga
     2641 gcagacgctg aataaagtga aagaggaaaa gcgaagagct gaacaagcag ggaataccga
     2701 aaaagcagct gaactcgaga agctggaatc gcaattggaa gcgatggtgc gcaaggatga
     2761 atctaccatg cagggttcgc gtacgcccaa tgtaattcag gaagaaaaga acgaaaagat
     2821 tgcagaaacc gttgatccgt cttacgacgc aaaagtggat gccatacaaa attccaataa
     2881 gtcggaagtc cgcaaaacag cagaaacacg cgaggccaat gaggagttga tccgcaagac
     2941 cgctgaagaa atcagtcgac tggaacgaaa cctctccacc ggaaatgtga gcgatcccga
     3001 agctacgcgt caacgtattg aggacttgaa agatcttcgt gatgagaaag agcgcgaagt
     3061 tgcgcaggct gataaacaat tggcagaagc aggaatcaca cgcgtggatg aaaacggtcg
     3121 ttcggtttcc ggttatgcat ttatgcctga gtattcgtcc gccgaagcac aaaagcgtat
     3181 cgatgctttg caaccgctta ttgataaagt acgcgatatt gaagaccagc tcaccgaatt
     3241 aaaaattcgt cgtgctgagt cgaacaatgc cgcagaatcg gatcgcatca cacaggagat
     3301 cattaagctc gaagcagaat tagtaaaact cgaattagag atcgcatcga aagttcagga
     3361 agcaaatcag ctggagaaaa aagagatcga agcgaaagtt tcaacagcca aatcggaagc
     3421 taaaaaagtt aaggtacctg ctgatgatcc tgaattcctc acggcccaga agctggaaga
     3481 aaaggcaaaa acgaagtttg aagatgcgaa aaaacttcgt gctgatgcag ccaaagaaaa
     3541 agatgagaag gccaggaacc tgctcctgat gaaagccaat gtggcagaac aattggggaa
     3601 taagtacctg aacaatgcgg caacttcgta ctccaaatct gcaaaaacag cgcagggtat
     3661 agaccctgaa cagaacattg ttaaggtgtc ggatatttat ccggaatatt tcactgctct
     3721 cgaaacttca tcctcttcgg gcgatcgaag caaacagacg gaagagcgga tacgattaaa
     3781 cgaagaattg atccgtcgta tcgatgcgca aatgtcggag cttaaatcta catctacttc
     3841 cgatcctgca acagccaata agctgaatca attgggggag ttgaaaaaac agaaagaaga
     3901 agaactcaag cgcgacaaac aaacactgga gcaactcagc gaacgtcccg aacttaccag
     3961 cgagaaaaag aatctgattc aaactgttga tcccggctat gttcaaagca tggagcgtat
     4021 tgaaaactcc acttcttcag aagcgcgaaa aatcagtgaa cggaatgatc tgaatgaaac
     4081 actgattgaa aaactggaca aacaaattgc cgaatccaaa gccattatcg caagtaccga
     4141 tgatgaaaag ataaagcagg atgaagaacg tcgtttgtct gaattggaga atctgcgcat
     4201 ggagaagaca cgcgaattat ctgcaggcag gattcgtctg gataaaatgg gaattgcttc
     4261 tttgcctgca caacagcagt atgaaaacaa atacccttac acttccgaaa aagcccagca
     4321 ggaaatgcag ggaattgaaa gtcagcttac cgccatcgaa gcaaaacgca agcagatcga
     4381 ggatttgaaa attcgtcgtt actcatccac cgataaggcc gagcagaaga aactcgacaa
     4441 cgaaatcaag aaagcggaat ctgagttgct cacaatggaa atgaatgttg ctgcatcatt
     4501 tatgagtgcc aatgctgcag agcgtgaagc gaccgaaaaa cgaatcagtg agaagaaaaa
     4561 ggaaacaggg tataaagata ttccggccaa tcaccaggtg atgaaaaatg taactgcgct
     4621 ggaacaacag gcagaacgca ttttttcgga agctgcagaa ttacgtcgca atgccaatgc
     4681 ggaaaaagat aagaacaaac gcaacgaatt gttccgtcag gctcatgcaa aagagcgtga
     4741 agcaaacagt ctcctggccg aagccgagca gcaactggat gttttattgg gcgacaaaac
     4801 acttgcatca gccaatcaaa aacaaaacac cggaaataac agcggacaag aagttgcaat
     4861 aaacacacaa cagggaggaa caacgcagca aggcggaaca acacaacaag gcggaacaac
     4921 gcaacagggt ggaacaacgc agcaaggcgg aacaacacag caaggtggga caacgcaaca
     4981 aggaggaaca acacaacaag gaggaacaac acaacaaggc ggaataagtt ctgctcaaat
     5041 ggcagatcct gccaatcgcg aaagtacacg gcaaatggct gaggctgatc gtttgcgcgt
     5101 gcagggaaat aatctcattg cccgtgcgca gcaactcgaa gactctgcca aaacggtcaa
     5161 aaagaaaaac cgtcaagctg ttcttgatca gtcggcgcaa aagcgaaaag aaggcgaaga
     5221 attattggcg cagagtgatc ggatgaatac aaaagccaac gaaacaagaa gacaggaaga
     5281 agagcaaata gcagagcaac gcagaaaaga agaggaaatg gagcgtgcaa ttgccattgc
     5341 ggatgatccg gaatatgccc gttacatgca attgcgcgaa gaagcaagaa gagcagaagt
     5401 ttcggcgaca aatgcagagc gcgattacca gcagcgacta aatgcaacag ccgatcttcg
     5461 taagcgcgca caggatacac gtgcacaagc ttctacagaa acggataacc aacgcaaaaa
     5521 tcgattgctc actcaggctt ccgatcttga acagcaagcc atccgtgcag aacggaatta
     5581 cgatactaca cgaaaccgcg caacacaacg tcgcacagaa gcaaaccgtg cacaaaccaa
     5641 tgccgataat tatctcgcag gaatggatcc gcgtaaagcg caaaccatcc gcgatcttgc
     5701 ttctgccgga aaattgccgg atcccaaggg aacatcgctt gctaccaata ccaccggtgg
     5761 aaccgcaaca acaaacggta accagcgcac tgtaaccaat gcaggtacca caagtgttgt
     5821 tagtccgatc accacaatgg atcctgaatt cgttgcgcgt aaatattctg tccccgaaaa
     5881 agtggaaact gaaatttatg tgctgtcgga aaaagcagtg tacaacaatg ccaatcctat
     5941 tcctgtaaat ccggaaatgc cgaaaggaat ttattttaaa gtgcaggtag gagctttccg
     6001 caatcagatt cctcaggaaa catttaaaga gtttgcacca attactgccg aaaccacgcc
     6061 gcagggactc acacgttata ccgttggata tttcaccacg tttaatgttg ccaacggcgc
     6121 aaaaatggag attcgcggaa tgggatatac cgatgcattt gttgttggtt tccgcgatgg
     6181 aaaacgtatt ccactcaacg aggcactgga agcattggga caaagcggtc aactcgcaac
     6241 aaatacccaa caaggcggaa caacacagca aggtggaacg acccaacagg gtggaacaac
     6301 gcaacaaggt ggaacaaccc aacagggtgg aactacacag caaggcggaa ctacacagca
     6361 aggtggaaca acacagcagg gtggaactac tcaggagttt gtaaacaact ttaatcctga
     6421 ccgtaacgcc accaattatt acaacgatcc gaatgcggcg aaagcgaatc aggtggaagt
     6481 cattcccgga ttgttcttta ccgttcaggt gggcgtttat tctcgtccgg tttcggctga
     6541 taaaatcttt aatctgaatc cgctcaacag cgaaaaaaca gcaaacgatc agattcgcta
     6601 tactacaggg gtcttcaccg atatcaatat cgctacttca cgcagggacg aaattcgcca
     6661 gattggtgta acagatgcat tcgttacggc ctatctcaat ggaaaacgaa taaccatcgg
     6721 acaagcacgc gattcgattg cgaaatacgg taattcaatt tttgtaaacg atcagaaact
     6781 ggtcagcaat acaacaacca cacaacaagg tggaacaacc caacaaggcg gaacaacaca
     6841 gcagggcgga acaacacaac aaggtggaac aacacagcag ggtggaacaa cgcagcaagg
     6901 cggaacaacg cagacccaag accgacagaa tgtaccgcaa gcttcgagtt acaaggttat
     6961 tcttggtcag tacaagggtg aagttccttc caaagaagcg gagattttcc tgaccaacat
     7021 gaattacggg atacgcaaag cgaatgtggg tgacggatct accgtttatt ttatcgataa
     7081 tgtttcatcg cgcacggaag ctttcaatat tgcagatcat tttattcaga aaggattgcc
     7141 cggtgcatat gttagcgaca aacttcctgc tcaaacgact gccacaaagg ttgaagaacc
     7201 acaagctgct tccactgtcc gattccaggt ttacattggt gaattcatag gcgaggttcc
     7261 gccggaagca acagaaattt tcctgaataa ctacgataaa ggagttaagc gcaaaaaact
     7321 gaatgatgga accatcgttt attatgtgct tgatggaagt tcctacgatg aagcattgaa
     7381 aatgaaagcc cattttatca gtgaaggact ttcttcggtt cgcattgtgg catttgacgg
     7441 atctaacgaa atcccgcttt caaaagctta cactgccggt atgcgttaca gaattgtggt
     7501 ggctgaattt gcaggtgaaa tgcctgagga agtgaaagaa gcatacggca atgttcccga
     7561 actcaatgtc cgtaccaaag ccattgatgg tggatttact atggcttatg ctgatggaat
     7621 taccgatatc gatcaggcct tgcgtctaaa ggcgcgtttg attgcggagg gactttctac
     7681 tgtgcgaatt gtagtggtaa aagacggaaa agaaatatct ttgtcggaag ctttccgatt
     7741 ggtctatgaa aaataatttt cgcttatcca ctcaaccccg ctggaaggaa gaagaggatg
     7801 tggtggttct tgaacaggat gttgatctgc gcagcatcat tctctttaac gataatgtga
     7861 atactttcgg ttatgtcatc gaaatgctga ttcgctattg caaacacgaa gctgttcagg
     7921 ccgaacagtg tacctacctg gttcattaca ccggtaaatg tgaggtgaaa agagggagct
     7981 acgatgaact ggaaccggtt tgcacgcaat tactcgaagc cggtttaacc gccgaaattc
     8041 aatgatcatg aaacaatttt ttttagctgt actgcttacc tccgttattg tatcctgtac
     8101 ccgtgtaccg gttaccaacc gcaagcagtt tcgtctattg caggaagagc aactcattct
     8161 catggcggat acagcctacc gcgattttct tgctactgcc aaagtgctgc cgcataacga
     8221 tcctcgcact gctacagtaa agcgggtagg agggaagatc caaagctcag tgatcgatta
     8281 cctcacccga aaaaacgcat taggacgcat tcgtggtttt acctgggagt ttaacgtggt
     8341 cgacgatcct accatcaatg cctggtgtat gccaggtgga aaagtggtgg tgtataccgg
     8401 aatcttaaac ctcgctaccg acgatacctt gcttgcagtt atcatgggac atgaaattgc
     8461 acatgccatt gcacgtcacg gtaacgaaag gatgagtcag caaatggcca ttcagggtgt
     8521 tggacgcgca ttgggaggag caatgtctga tccggtgaaa cgcaatattt tcctgcaatc
     8581 gtacggaatc ggttctgcac tgggaattct ctcttatagc agaaaacacg aaacggaatc
     8641 ggataaaatg ggactcgtct tcatgtatct cgcgggatac aatccttcaa aggcgattga
     8701 tttctggaaa aaaatggccg ctaacggagc agctgtgcct gagtttttga gcacccaccc
     8761 cagcgatgaa acgcgaatca aggacatcac cgattttctg cctttgattc cgaaatacgc
     8821 tcagtgatct ttctcactgt gtaacaagta ccggcaaact aactttgcag aaaaataaga
     8881 ttatgcctac agttaagctg acagccgaaa aattcaaatc cgatattttc gattatacca
     8941 ccgaaaagga atggaaatac aagggaaatg tccctgccat catcgatttt tatgccgact
     9001 ggtgcggtcc ctgtaaaatg gtggcccctt tgttggagga attgagtgat gaatacacgg
     9061 ataagctcat tatttacaag gttgataccg aagtggaaca ggaactttct gccgtatttg
     9121 ggatccgaag tattcccagt ctcctgttta ttccccgcga agcgcagcca atgatgcagg
     9181 ccggagcttt accaaaagca gcgttcaagg agattattga ggaagaattg ttgaaataaa
     9241 agctcgtttc tgcgccttag accggttttg ccgaatgatt tattaacagc ccttgataac
     9301 ctgatgtccg gttcgtccat tattgtttat ttttggactt cttcatcaaa catcaaaaag
     9361 aatgaattta acaggcatca tttcaatttc gggtaaaccc ggtctttaca aagttgtttc
     9421 tcaaagtaag aacagtctga tcgtagaagg tcttaccgac aaaaagcgtt ttcctgcatt
     9481 ttctgccgat aaggtaagtg cgctggacga catcagcatg tacactacag aggaagatgt
     9541 tccgctggca gagatcatcc gtaagatctg ggataaggaa aaaggcggac gttgtgtgga
     9601 tgctaaggaa gaagccaatg cacatcacaa gtattttgcg gagatcctcc cgaattacga
     9661 tcgtgagcgt gtgtacaaca gtgatctgaa aaaacttttt tcctggtaca acctgcttca
     9721 ggaaaccggg actttgaaaa acctgattga agaagccgaa aaagctgctg cagcagagaa
     9781 ggaagaagcg aagccgaaga aaaaggcagc gaaggaagag aaagctacag aggaaaaagc
     9841 ggaagtgaag gaaagcaaag ccaaagaacc caaagcgaaa aaacctgctg cggaaaagaa
     9901 agaagtgaag aaaacagctg cgccaaaagt gaaggccgaa tcaaaagcca aaaccacagt
     9961 gcgcactgct tccatgaaac gtggcgcata agccatggcc agcacaggta ccgatcttcc
    10021 ggttcgacca ccacgtcgtt tcattgcaca ggatttgcag atcgacgcat ggtcgaagct
    10081 ggaaccttac ttcagtacat tactgaatcg cgatttgctt tcgattggcg atcttgaaca
    10141 atggttgcag gatcgcagcg agttggaagc tgttgtagaa gaagaaggtg cctggcggta
    10201 catccgcatg accatcaaca ccaaggatga taaggccgtt gaagcttacg aatttttcgt
    10261 aaccgaaatc gagcctcgta ttgcacccta tgccgataaa ctcaaccgga aaatgatgga
    10321 ttgttcattc gtgaacaaac ttgaaaaacc cggttactcg atttaccgca gagccattgc
    10381 acaggagtta aaactcttcc gcgacgaaaa tgttccgctt atcgctcaga tccaaaaaga
    10441 atcgcagcga tacggtgcca tcaccggcgc acaaagtgt
//
LOCUS       DAOMYG010000038        10418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2132_length_10418_cov_8.912381, whole genome shotgun sequence.
ACCESSION   DAOMYG010000038 DAOMYG010000000
VERSION     DAOMYG010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10418
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2132_length_10418_cov_8.912381"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PK610_02205"
     CDS             <1..350
                     /locus_tag="PK610_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34765.1"
                     /translation="LRDVESLLVQHGEWIGRTRSTAPSIEEITDEYGLFNSGWEIDLV
                     CTKPGCRKKLQPSDLDLNYEKWDAAIKSAFDYFEKVDQPLLKYMEPEIKDKLNRFLKE
                     GRDYISVLELKIK"
     gene            complement(352..762)
                     /gene="ruvX"
                     /locus_tag="PK610_02210"
     CDS             complement(352..762)
                     /gene="ruvX"
                     /locus_tag="PK610_02210"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRJ34766.1"
                     /translation="MALGIDYGTKRVGLAISDELGMIASPLETIHSTELTAWLTRNIP
                     LRKIKTVVVGEPKKLDGSDTDSTQLINELCVHLRRTFPQVEIVRVDERFTSSIAMQSM
                     IIGGASKKQRQNKGAIDAISAALILRSWLESPKR"
     gene            complement(765..1286)
                     /locus_tag="PK610_02215"
     CDS             complement(765..1286)
                     /locus_tag="PK610_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRJ34767.1"
                     /translation="MRLYLVGFMGSGKSTVAKLLAEDMNLTAFDLDQIIIEESGLPVS
                     ILFKQEGEQAFRIRESKLLDQWIKSNAHGVLATGGGTPCFLGNGRKMEMSGVMIYLKT
                     SADTLSRRLNDQMESRPLLAGLKGKELFSYIEHTLAQRSEFYERARIIVNADQTTNDI
                     VNEIKNKLKEVDL"
     gene            1434..1506
                     /locus_tag="PK610_02220"
     tRNA            1434..1506
                     /locus_tag="PK610_02220"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1467..1469,aa:Lys,seq:ttt)
     gene            complement(1602..2582)
                     /locus_tag="PK610_02225"
     CDS             complement(1602..2582)
                     /locus_tag="PK610_02225"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRJ34768.1"
                     /translation="MSNDVSKNIRLGVFVIAGTAFLIAALYFIGNKQNFFGNTITIST
                     RFYDVGGLMKGHNVRYSGIDVGTVKNIEIVDDTSILVTMVIDKESAKFIKKNAFASIG
                     TDGLMGNRLVNINSIAEPSSSIENGDQIQSIRSVEMDEMIRTLAITNQNIMLITADLK
                     RMTEKINNSKSLWGLLADTTMAVNTTSILDNLAASSKNAAVFSNDMIRISQGLKAGKG
                     LVGALLYDTAYVSNLAQTLTNFQNAADSVQMISGDIRQITYKIQSGGGVASSVLSDTS
                     LVNDLEASMKNLKSGSASFSENMEAMKHNFLFRRYFRKQAKQKEKKEMKK"
     gene            complement(2584..3348)
                     /locus_tag="PK610_02230"
     CDS             complement(2584..3348)
                     /locus_tag="PK610_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ34769.1"
                     /translation="MMENKNIAKAIQSDEIVMSIKGLCKSFGSNHVLRDFSLNLFRGE
                     NIAVIGKSGSGKSVLIKCVIGLLKADKGSITVLGEDVGNLNRRQLDEMRVKVGFLFQS
                     NALYDSMTIRENLEFPLRRHRRKDKPENVDDLVMEALSNVGLEETVDMMPSELSGGMR
                     KRIALARTLILKPEIILYDEPTTGLDPITGREISQLMVEIQKKYNTSSIIISHDMNCV
                     KIASNRIAMLIDGTNYATGPFDELKEFNDPKVRSFF"
     gene            complement(3348..4115)
                     /locus_tag="PK610_02235"
     CDS             complement(3348..4115)
                     /locus_tag="PK610_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ34770.1"
                     /translation="MRFKGLRNILFETGDISRFAGRFFAEAFVPRYEFREFLRQSYYI
                     GYKSLPLVALTAFIIGLVITIQSRPTLVEFGAEAWLPQMISVSLIREIAPVITALICA
                     GKIGSGIGAELGSMKVTEQIDAMDVSGTNPYKFLVVTRVLATTLMIPVLVVFADAISL
                     YGGYLGANIRGVVSWDLFWNQVFESVTFNDLLPSLLKTFFFGFAIGIVGCYKGFSTQK
                     GTEGVGRSANSAVVVSSILVFIIDLIAVQIADLMGLT"
     gene            complement(4321..6018)
                     /locus_tag="PK610_02240"
     CDS             complement(4321..6018)
                     /locus_tag="PK610_02240"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HRJ34771.1"
                     /translation="MKKKYLLLVAATAGLLSTGAFAQTARVQVIHNSADAAAATVDVY
                     ANGAMFIDDFEFRTAFGFADVPAGIPLTIDIAPGNSTSAAQSIASFPVTFTAGETYVV
                     VANGIVSGTGYSPAPAFGLDVYTLGRESANVATNTDVLVMHGSTDAPTVDVVVPGVGP
                     IVNDASYGDFAGYLEVAAADYTLNITDASGAVVVRSYQAPLSTLNLDGEALVVVASGF
                     LNPANNSNGAAFGLWVALPSGGALVELPESNARLQVIHNSADAAAAQVDVYLDGSLLL
                     DNFAFRTATPFIDLTAEVSHSIAIAPSTSTSVAQAIATFPVVLEANETYVVVANGIVS
                     GTGYSPAPAFGLDIYQGARESAGNAANTDILVAHGSTDAPIVDVKAGSVTLVNDIAYG
                     EFDGYLEVPTSNLTIDITDQSGSVVVASYAAPLQTLSLQGDAITVVASGFLNPANNSG
                     GASFGLWVALSTGGALVPLGNVTSVEEENEVSQMEIFPNPANNNFQLGITILNESLVS
                     VNIVDMQGRIVFAANHGVLSKGNHVISLDASELSAGTYVITVLANGSTKNLRLMVQH"
     gene            complement(6218..7411)
                     /locus_tag="PK610_02245"
     CDS             complement(6218..7411)
                     /locus_tag="PK610_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRJ34772.1"
                     /translation="MSEHRNEALDRLGIPRNNRIILSLDGGGIRGILTLQLLKKMEEV
                     AGYPLHRFVDMVAGTSTGGIMAGLIALGYSAEEIEKKYVELVTEVFLKRGALANRFLN
                     PPLYSKANYRRMLKEIIEDKTLQDTVIATETDIMITAKDLAASEETFFSCFSRSDGYN
                     GTYKNVLLRAVMEATMSAPTYFTPLERFVDGGTTTYNNPTLAAVMEAVGYGGKGKYSS
                     DQLTVFSFGTGTSVKFVKPEETGNPKGMDVAFWLNYIMDEASQDAADMQMDVLRSGLI
                     SGLDLRRFQLSLDQNSIRRLPNRRINRVKGIKAKWMWDLSNEELDGIGLDEVGKFGLM
                     QEIGEAMAEYVCPTHDVSREKGNWFRKDLINGKRRDELVSTFGDIERIKTQMSDPDWL
                     DAFPE"
     gene            7466..9094
                     /locus_tag="PK610_02250"
     CDS             7466..9094
                     /locus_tag="PK610_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRJ34773.1"
                     /translation="MSMIRSLRSSYKGWLLAGIVLLLLIAIPLVILFSSLFTTPQSSK
                     AILEQTLPGEAFFNTLALCVIAAVVSLFLGGGTAWLVTQYQFPLRKFFSWSLALPLTI
                     PGYILAFTYAGIFSFSGPLQTVIRNQFGQDRAQALHFDIMNLPVLGLLFALALFPYVF
                     LSAKAAFSIQSARQIESARSLGKKGSSILMKIALPLSRPALAAGVFLVMMEVINDYGA
                     ASYFGIRTYTTALFRTWGYDIQAALLLAGWILLFVFGLLIAERLLRGKARYQNTARQS
                     YVPITPLKGFRAWTATFLCSIPFVFGFLIPFSFLGYWAMQTAPKIMNDHFFVLTWNSI
                     QLAFIATIVIVVVALILAYVRKLHQSFSGKIIAQFINVGYAIPGAVIAVGVLAFSGWS
                     DAMIAHFGSAKGLFFTGSIAMLIFAYLVRFLAVAYQPLEAGLEKNHGSLGESSRSLGK
                     SPLRTLLFVELPLLRNTVLVAAIMVFVDVMKELPLTLILRPFNFETLATETYRHAKIM
                     ESVPESSSAALILIFIGAIPVFVLSRLMTEKHGT"
     gene            9084..10046
                     /locus_tag="PK610_02255"
     CDS             9084..10046
                     /locus_tag="PK610_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ34774.1"
                     /translation="MGLELKNISRSFGKNRFALSGINFSMQKGEILALLGESGSGKST
                     LLRIIAGFELPDKGSINLSGKTICDEKISLHPEKRNIGLVFQDYALFPHLNIEKNIAF
                     GQKKNPSRTTNEWLALVGLPGMGKRYPHELSGGQQQRVAIARSLAAEPELLLLDEPFS
                     NLDDSIRSSVRGEIRDIIRSTNTTAVFVTHDTRDCMAVADKVIVLSNGKLLQEGDPKQ
                     LYIQPCNSYVARIFGEVNIVEGEIARQLSGLKKFGIRPQHIIPGNDFSCTVLSCRFDG
                     DHYDLELDLNGSKLHFHSDQPYTKDEVIRISFPKDKIIDLSHES"
     gene            10036..10353
                     /locus_tag="PK610_02260"
     CDS             10036..10353
                     /locus_tag="PK610_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34775.1"
                     /translation="MKAERLTMIRQMLDQNPDDPFLKYAAALEYKKAGNISECIGLMQ
                     KLMIDHEDYLPVYFQYGKLLEEKGNIEEAIHIYKKGCDLAEKQNEMKTLRELKEALVL
                     LED"
BASE COUNT         2976 a         2287 c         2118 g         3037 t
ORIGIN      
        1 cgctgcgcga tgtcgagtcg ctgctcgtgc agcacggcga atggatcggc cgcacgcgtt
       61 cgacggcgcc ttccatagaa gagattaccg atgaatacgg actttttaat tccggttggg
      121 aaattgactt ggtatgcaca aaaccgggat gccgaaaaaa attgcagcca tcggacctcg
      181 atctgaatta tgaaaaatgg gatgcagcaa taaaaagtgc attcgactat tttgaaaaag
      241 ttgatcagcc tctattaaaa tacatggaac cagaaattaa ggacaagctg aatagatttc
      301 tgaaagaagg tcgcgattac atttccgtgc ttgaactaaa aataaaatag ttcatcgctt
      361 aggcgattcc aaccatgagc gcagaattaa tgcggcactg atagcatcga ttgcaccttt
      421 attctgcctt tgctttttac ttgctcctcc aataatcatg ctctgcattg ctatggatga
      481 ggtaaatcgt tcgtcgacac gcacaatttc aacctgtgga aatgtccgtc gcaaatgaac
      541 acagagttca ttgatcaatt gagtggaatc tgtatcggaa ccatccagtt ttttaggttc
      601 accaacaact acagttttaa tttttcgcag agggatgttg cgtgttaacc aggcagtcag
      661 ctccgtagaa tgaatcgttt ccaagggact cgcaatcatt cccaattcat ctgaaatggc
      721 aagtcctact cttttggttc cataatcaat accgagggcc acgctcataa atcaacctcc
      781 ttcagcttgt tctttatttc gtttacaata tcgtttgttg tttgatcagc attgacgata
      841 attcttgctc gttcataaaa ctcactgcgc tgagcgagcg tatgttcgat ataggagaac
      901 aactccttgc cttttaatcc tgccaataaa ggacgcgatt ccatttgatc gtttaaccgc
      961 cgggagagtg tatccgcaga agttttaagg taaatcatca cacccgacat ctccatttta
     1021 cgaccattcc ccaaaaaaca aggcgtacca ccaccggttg ccaaaacgcc atgcgcgttt
     1081 gattttatcc actgatccag cagttttgat tcgcgtattc gaaaagcctg ttctccttct
     1141 tgtttaaaaa gaatagaaac aggtaatcca ctttcctcta tgatgatctg atcgaggtcg
     1201 aaagcagtca aattcatgtc ctctgccagc agttttgcta ctgtagattt accagaaccc
     1261 atgaatccga cgagatataa acgcatgttc aaaactaatg cttttaccga aactcctgat
     1321 ttttcaggga ttttagggtg agattcgata cagattcatg tttttttcat tctgcttttg
     1381 gaaaacagaa tcaagccatt atatttgcac ccgctttcaa aggaaagtgt attgatttcg
     1441 tagctcagct ggtagagcac aacactttta atgttggggt cctgggttcg agccccagcg
     1501 gaatcacaga aaagcccgag gcagataaac atcgccacgg gctttttcat tttataaaag
     1561 ttggataatc agtaaaaatc tcaacaagaa aaaaggaacg attatttttt catttccttt
     1621 ttttccttct gtttcgcctg ctttctgaaa tatcttctga aaagaaaatt atgtttcatc
     1681 gcttccatat tctcactgaa agacgcagat cctgatttca ggtttttcat gcttgcttcc
     1741 agatcattta caagggatgt gtcggaaaga actgatgatg ctactcctcc acccgactga
     1801 attttatagg taatctgtcg aatatcgccg gatatcatct gcaccgaatc tgctgcgttt
     1861 tgaaaattcg ttagcgtttg agcaagattg gatacatatg cagtatcgta aagtaaagca
     1921 ccaaccaagc cttttcctgc ttttaatcct tgcgatatcc ggatcatatc gtttgaaaac
     1981 accgctgcat ttttgcttga tgctgccaaa ttatcgagga tactggttgt gtttacagcc
     2041 atagtggtat ctgccagaag tccccacagc gatttggaat tattaatctt ctcagtcatt
     2101 cgtttaagat cggcagtgat gagcatgatg ttttggttgg taattgccag agtacggatc
     2161 atttcatcca tctccaccga acgaatactt tgtatttggt caccattttc gatagaagag
     2221 gatggctcgg caatgctgtt gatgtttacg agacgattcc ccataagtcc atctgtgcca
     2281 atactcgcaa aggcattctt cttaatgaat ttggcagatt ccttatcgat aaccattgta
     2341 accaaaattg aagtatcatc gactatttca atgtttttca ctgtacctac atctataccc
     2401 gagtatcgga cattatggcc tttcatcaat ccaccaacat cgtaaaagcg ggttgagatc
     2461 gttatcgtgt taccgaaaaa attttgcttg tttccaatga aatacaatgc ggcaatcaga
     2521 aatgctgttc cggcaattac aaatacaccg aggcggatat ttttagatac gtcgttactc
     2581 atactagaaa aatgatctga ctttagggtc gttaaattct tttaactcat caaatggacc
     2641 ggttgcatag tttgttccat cgatgagcat tgcaatgcgg ttggatgcaa tttttacgca
     2701 attcatatcg tgcgaaataa taatggaaga tgtgttgtat tttttctgaa tctccaccat
     2761 gagttgacta atttctcttc cggtaatcgg atcgagtccg gtggttggtt catcgtaaag
     2821 gataatttcc ggtttgagaa ttaatgtccg tgccaatgca atccgtttcc gcatccctcc
     2881 ggataattct gagggcatca tatcgacggt ttcctcaaga ccaacattac tcagtgcttc
     2941 cattacaaga tcatccacgt tttccggttt gtctttgcgt cggtgacgtc gtaatggaaa
     3001 ctcaagattt tcgcggatgg tcattgaatc gtacagtgca ttgctttgaa agagaaatcc
     3061 aacttttacc ctcatttcat ccaattgccg tctgttcaga tttccgacat cttcacccaa
     3121 tacagttatg gaacctttat ctgcttttag caaacctatc acgcatttaa tcagcactga
     3181 ttttcctgag ccggattttc caattactgc aatattttct cccctgaaaa gattaagact
     3241 aaaatcgcgc aatacatgat tgctgccgaa tgatttgcaa agtcccttaa ttgacattac
     3301 tatttcgtcc gactgaatag cctttgctat gtttttgttt tccatcatca ggttagtccc
     3361 atcaagtcgg caatttgtac tgcgatcaga tcaataataa aaacgagaat agatgaaaca
     3421 acaacagcag aatttgcaga tcgtccaaca ccttctgtac ctttttgagt tgaaaatccc
     3481 ttatagcaac ccactattcc gatagcgaaa ccaaagaaaa atgttttcag tagtgagggc
     3541 aaaagatcat tgaaggtaac actctcgaaa acctgattcc agaaaagatc ccaactcacc
     3601 acacctctga tattggcacc cagatagccg ccgtacaatg aaattgcgtc tgcaaacacc
     3661 actaaaaccg gaatcatcaa tgttgttgcc aacacacgcg taaccaccaa aaacttatac
     3721 ggattggtac ctgatacatc catggcatca atctgttcgg tcactttcat ggaacccaat
     3781 tctgcgccaa taccggaacc aatttttcca gcgcagatca aagccgttat taccggagca
     3841 atttcacgaa tcagtgaaac agaaatcatt tgcggcaacc atgcctcagc accaaattcc
     3901 accaatgttg gtcgcgactg aatagtaatt accagaccga tgataaatgc agttaatgct
     3961 acaagtggta gtgatttgta accgatataa tacgattgac gcagaaattc cctgaattcg
     4021 taacggggga caaaagcctc agcaaaaaag cgacctgcaa atcgggaaat atccccggtt
     4081 tcgaataata tgttgcgaag gcctttaaat ctcatccgac aaaattgctt tcgaaatttc
     4141 agcaaatcaa catgccggcg gaatgagtag ggtcatacca caaaactgat aaagatcata
     4201 atttacaaaa aatccgtaac tcaactgcaa acaggaaatt ggaaaggaga tctgagtctg
     4261 taaaaagaat aaaccccgac gtttccgccg gggtccttcc aaaccaaatc agcaagtaga
     4321 tcagtgttga accatcagtc gaaggttttt tgtcgatcca ttcgctaata cagtaataac
     4381 atatgtacct gcagaaagtt cagatgcatc taaagaaata acatgatttc cctttgataa
     4441 tactccatga tttgcagcaa acacaatgcg tccctgcata tctacgatat tgacgcttac
     4501 cagggattca ttcaggattg tgatacccag ttgaaaattg ttgtttgcag gatttgggaa
     4561 aatttccatt tgagaaactt cattttcctc ttcaacagaa gtaacatttc ccagtggaac
     4621 aagtgctccg cctgttgaca atgcaaccca caagccaaag gaagcgccac cgctattatt
     4681 tgcaggattc aggaatccgg atgcaacaac agttatagca tcaccttgca gactcaatgt
     4741 ttgcaatggc gctgcataag aagcaacaac tacagaacct gattgatcgg taatatcaat
     4801 cgttaaattt gaagttggaa cttccagata accgtcgaat tctccatagg cgatatcgtt
     4861 caccagtgtt actgaacccg ccttcacatc aacaattgga gcatcggttg aaccgtgtgc
     4921 aacaagaata tctgtattgg cagcattgcc cgcagattcg cgcgcacctt gatagatatc
     4981 gagtccgaaa gcaggtgccg gagaataccc tgttccgctt acaattccgt tggctacaac
     5041 tacatacgtt tcgtttgctt ccaatacaac agggaatgta gcaattgcct gcgcaacact
     5101 tgttgatgta gaaggagcga tggcaataga gtgacttact tcggctgtaa gatcaataaa
     5161 tggcgtagct gtgcggaatg cgaaattatc aagcaagagt gaaccgtcca gatacacatc
     5221 tacctgcgca gctgcagcat cagcagaatt gtggatcacc tgcagacgtg cgtttgattc
     5281 cggaagttca acgagtgctc caccggaagg aagggcaacc cacaatccga atgctgcacc
     5341 gttgctgttg tttgcaggat tcaggaatcc ggatgcaaca acaactaaag cctctccatc
     5401 aagattcaac gtgctcaaag gagcctggta agaacgaacc acaactgcac ctgaagcgtc
     5461 tgtaatgttc agagtataat ctgctgccgc tacttcaagg tatcctgcaa aatcaccata
     5521 ggacgcatca tttacgattg gtccaacccc cggcacaaca acatctacag taggcgcatc
     5581 tgttgatccg tgcattacca gcacatcggt attggtcgct acattggcag attcacgacc
     5641 taatgtatac acatccagtc cgaatgcggg tgcaggacta tatcccgttc cgcttacaat
     5701 tccgttggca accactacat aagtttcacc ggctgtaaat gttaccggaa acgatgctat
     5761 agattgtgca gcgcttgtag aatttccggg agcgatatca attgtaaggg gaattccggc
     5821 agggacatcg gcaaatccga aggccgtacg gaattcaaaa tcatcaataa acattgcacc
     5881 attggcataa acatcaactg ttgcagctgc tgcatctgca gaattgtgga tgacctgcac
     5941 cctggcagtt tgggcaaagg caccagtaga tagcaaacct gctgttgccg ctactagtaa
     6001 taagtacttc tttttcatct ggattggttt tggttaatag ttgtacaaac aacatatcca
     6061 gatgaagaat tgtttaatca aaatgataaa aggttaaaca aaacacttac gaactcaaat
     6121 taaaacactg aaatacagct gtataaattt tttattattt tagaaacggt cgggtttaaa
     6181 gaggaaacgg ctcggtaata aaggcctaat tatggtgtta ttccggaaac gcatccagcc
     6241 aatcgggatc actcatttga gttttgatcc gctcaatatc gccaaaggtt gataccaatt
     6301 catcgcgcct ttttccattg atgaggtctt ttcggaacca attccccttt tccctcgaaa
     6361 catcgtgagt cggacataca tattcggcca ttgcttctcc aatttcctgc atcaatccaa
     6421 atttcccgac ttcatccaaa ccaataccgt ccaattcttc attgcttaaa tcccacatcc
     6481 attttgcttt aatccctttt acacgattta ttctgcggtt gggcaatcga cgaatggagt
     6541 tttggtcgag cgataactga aaccgcctaa gatcgagtcc tgaaataagt ccgctgcgca
     6601 acacatccat ttgcatatct gcggcatcct gactggcttc atccatgatg tagttcaacc
     6661 aaaatgcaac atccattcct ttcgggtttc cggtttcttc aggctttaca aatttcacac
     6721 tggtacctgt accaaaactg aatacagtga gttgatcgga agagtatttc ccctttccac
     6781 cataacccac ggcctccatc accgctgcaa gggtaggatt attgtaagta gtagtaccgc
     6841 catcaacaaa gcgttcgaga ggagtaaaat acgtcggagc cgacattgtt gcttccatta
     6901 ctgcacgaag caaaacattc ttatatgttc cattgtatcc atcgcttctg gaaaagcagg
     6961 agaaaaaagt ttcttcgctt gcggcaaggt cttttgcggt aatcataata tccgtttcgg
     7021 tagcgatcac agtgtcctga agtgttttat cttcaataat ttctttcagc attctgcggt
     7081 aattcgcttt ggagtataat ggcgggttga gaaacctatt ggccaatgct cctcttttca
     7141 ggaatacttc ggtaacgagt tctacatatt tcttttcaat ttcttcagca gaatatccca
     7201 aagcaatgag tcctgccatt attcctccgg ttgatgttcc cgctaccata tcaacaaagc
     7261 ggtgtaaggg atagcctgca acctcttcca tttttttgag cagctggagt gtaagaatgc
     7321 cccttattcc tcctccatcc agagaaagta taattcgatt gttccgggga attcccaggc
     7381 ggtcgagtgc ttcgttgcgg tgttcggaca tggttttttt attatttcta ccgaaaatac
     7441 gattttgtaa gactattttt gagaaatgtc gatgatacga tcactccgct caagttacaa
     7501 aggctggctc ctggcaggaa tcgtacttct gcttttgatc gcgattcctc ttgtcattct
     7561 tttttcatcg ctatttacca caccacaaag cagtaaagct attctcgaac aaacattacc
     7621 gggagaagca tttttcaata cgcttgcgtt gtgcgtaatt gccgcagttg taagtttgtt
     7681 tttgggtgga ggcactgcat ggctcgttac acagtaccaa tttcctctgc gtaaattttt
     7741 ctcgtggtcg ctggcacttc cactcactat tcccggatat attcttgcat ttacctatgc
     7801 aggaatattt agtttttccg gtccactgca aactgtaatc cgcaatcaat ttggccagga
     7861 tagagcacaa gcgctgcatt tcgatatcat gaatcttcct gtgttgggat tgttatttgc
     7921 tcttgcgctt tttccgtatg ttttcctcag tgcaaaagcc gctttctcta ttcagtcggc
     7981 acgccaaatt gagagtgcaa ggtcgcttgg aaaaaaggga tcgagtattt taatgaagat
     8041 cgccctaccc ctttccaggc ccgcacttgc tgccggagtt tttctggtta tgatggaagt
     8101 gatcaacgat tacggtgctg ccagttattt tggaattcgc acttacacta cagcattgtt
     8161 ccgcacctgg ggttatgata ttcaggctgc acttttactt gccggttgga tcttactttt
     8221 tgtattcggc ctcctcattg cagaacgctt attaagaggg aaagcccgct atcaaaacac
     8281 agcaagacaa tcgtatgtac caataacacc tttaaagggt tttcgtgcat ggactgcaac
     8341 atttttatgc agcattccat ttgtatttgg atttttgatt ccatttagtt ttttaggcta
     8401 ttgggcaatg caaactgctc ccaaaataat gaatgatcat ttttttgtgc taacctggaa
     8461 cagtattcag cttgcattca ttgcaactat tgttattgta gttgttgcat taattctggc
     8521 ttatgtgaga aaattgcatc aatcgttcag cggaaaaatt atcgctcaat tcattaacgt
     8581 aggttatgct attcccggtg ctgtaattgc agtaggagta cttgcgttct cgggttggtc
     8641 ggatgcaatg attgcacatt ttggttctgc gaagggtttg ttttttactg gaagtattgc
     8701 catgttgatc tttgcctacc tggtgcgttt tcttgctgtt gcctaccagc cattggaagc
     8761 aggacttgaa aaaaatcacg gttccctcgg agaaagttcc cgttcgctgg gaaaaagtcc
     8821 tttgcgcact ctattatttg ttgaattacc cttattgcgt aacaccgttt tagttgctgc
     8881 tataatggta tttgtggatg taatgaaaga attgccactt acgctaattc tcagaccctt
     8941 taattttgaa acgcttgcaa cagaaaccta cagacacgcc aaaataatgg aatctgttcc
     9001 ggaaagttca agtgctgcat taatccttat ttttattggc gccattccgg tatttgtgtt
     9061 aagccggtta atgacagaga agcatgggac ttgaactaaa aaacatatcg cggtcgttcg
     9121 ggaaaaatcg ttttgcgtta agcggaatca atttttccat gcaaaaaggt gaaatacttg
     9181 cacttcttgg cgagagtggt tcaggcaaat cgacattgct ccgcataata gcaggattcg
     9241 aattaccgga taaaggatcc ataaacctca gcgggaaaac catttgcgat gaaaagatat
     9301 cactgcatcc ggaaaaaaga aatatcggtc tcgtttttca ggactacgca ttgtttccac
     9361 atttgaatat tgagaagaac attgcatttg ggcagaaaaa aaatccttca cgtaccacca
     9421 atgaatggct ggcactggtc ggtctgccgg gaatgggcaa acgttatccc cacgaattat
     9481 caggtggtca gcaacagcgc gttgccattg ctcgatcgct cgctgcagaa ccggagttgt
     9541 tgttactgga cgaaccattc agtaatctcg acgacagcat tcggagttct gtaagaggag
     9601 aaatccgtga tatcatccgg tcgacgaata caactgcagt atttgtaacc catgacaccc
     9661 gcgattgtat ggcagtggcg gataaagtca ttgttttgag caatggtaaa ttattacagg
     9721 aaggagatcc gaaacaatta tacattcaac cgtgtaacag ttatgtagca cgaatttttg
     9781 gtgaagtaaa tattgtggaa ggagagatcg cacgtcagtt aagtggatta aagaaattcg
     9841 gtattcgtcc acagcatatt atccccggta acgatttctc ctgcaccgtt ctttcctgca
     9901 ggtttgatgg tgatcattat gatctggaat tggacttgaa cggaagtaaa ctccattttc
     9961 attccgacca gccgtataca aaagatgagg taatacgtat ttcgtttcct aaagataaaa
    10021 tcatagatct gagtcatgaa agctgagcgt ttaaccatga tcaggcaaat gctggatcaa
    10081 aatccggatg atccgttttt aaagtatgca gctgctttgg aatacaaaaa agcaggaaac
    10141 atcagtgagt gtattggttt gatgcaaaaa ctgatgatcg atcatgaaga ttatctcccg
    10201 gtttattttc aatacggcaa attgctggag gaaaaaggaa atattgaaga agccatccac
    10261 atttacaaaa aaggatgtga tctggccgaa aaacaaaacg agatgaaaac gttgagggaa
    10321 ttgaaagaag cattggtgtt gctggaagac taaaccctcg aatgcatttt accaggcaga
    10381 taataaacag aagtctgcag ttattcaagt cagcttct
//
LOCUS       DAOMYG010000039        10406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2134_length_10406_cov_11.559946, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000039 DAOMYG010000000
VERSION     DAOMYG010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10406
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2134_length_10406_cov_11.559946"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PK610_02265"
     CDS             complement(<1..688)
                     /locus_tag="PK610_02265"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRJ34776.1"
                     /translation="MKNTIITAIAFVTGLGSLTVANAGNEDRVGSAGASELLINPWAR
                     SATWGSAGISSVTGIEATFMNVAGLAMTKKTEVNFVSSNWLTGSGIRMNQAGLAQRVG
                     ETSVIGLTFNSMTFGEIPITTVDIPEGGIGTFSPRYFNIGFSYAKEFSNSIYGGLTVK
                     ALSESIANVKGSGVCFDAGIRYVTGEKDQIKFGINLKNVGPPMKYSGDGFSYLVTLSN
                     GMQMTTEQRSA"
     gene            complement(729..4883)
                     /locus_tag="PK610_02270"
     CDS             complement(729..4883)
                     /locus_tag="PK610_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ34777.1"
                     /translation="MNRKGIMRLGTGLVLGLLATSQLRAEIDPALRNASGSTTGMQQR
                     AAGCAPATALTKLEFNNVSAIIETGGNMWQDRAQGFSAYEVPKGSRLRVMFAGALWLG
                     GKDFNDQLKVAAVTFRQGNDFWPGPLLTDGTAEVDQATCLYYDKFFVTLRQDIVTFVA
                     YTECIQSGAPNCGDLFPGYTVPRSILEWPGNNSAAGYDFNLAPYKDVNNDGVYNPGDG
                     DYPWYDIKKELACGNDRTVTLYGDQNFWWVFNDKGNIHTETGADPIGMEIRAQAFAFS
                     TNDEVNDMTFYNYELYNRGTQTLFNTYFGQWCDPDVGFSEDDFVGCDVQRGLGYAYNG
                     DAFDDAGQGQSGYGANPPAVGIDFFQGPFQDNDNIDNPLTTNISDAIDSLGIPYKGIG
                     IGYGDGVVDNERFGMRKFLYYFRSDQTNVNAQKEPNNANDFYRYLQGIWLDGTPFYFG
                     GTAHQSSAGAQANGLVRCDFMFPGNSDPLLFGTLGVPVANASSWTEFNEGNTPADRRF
                     MQSAGPFTLTPGQKNNITVGVVYARATTGNSYASVDKLRLADDKAQALFDNCFKILDA
                     PDAPDLTIQELDRGVVLYLTNPNSSNNVGEAYVEKDPFIVLPFVLENGDSTATYTDAK
                     RDSLSSYRFQGYKIYQLKDATVSSSDLTDPDKARIVAQCDIKDGVGRLVNFIKDEDLN
                     YDVPTMMVEGADKGIFHSLYLENDAFTQKRLVNHKTYYYMAVAYAYNNYKTFDPYDAT
                     KLDGQKKPYIESRKSVTGAIRAVSAIPHIPVPETAGTNQVAKYGDGPEITRIEGAQNG
                     SLFLDLTAESENFIVQNFKMATPTYQAGRGPISVKVIDPLNVKPGSFILEFLPDATGG
                     LVASNWRLKAAQAMTINGTTYAINDVVVSSSSTIRINNEQLIPELGISIAIEQYKYTT
                     ISGSQQYTEPISGEIFFADSSKRWLVGVPDGEGSGPQNWLRAGTQYVACDAGAYPDPQ
                     NDPCNYNDYQGLDNTESYENLAGGTWGPYRLCASSINSGGVEFPAATAVGSGYAGTVG
                     TLGSAASLHTLPNVDIVFTSDMSKWTRCPVLEMQESAALSQGGRTKQKMRAAQSVDKF
                     GNPDGTGTGMGWFPGYAIDVATGERLNMAFGEDSWLGVDNGRDMKFNPTNRLYSNLGD
                     PIFGGKHYVYVFTNQDREIPFADRMPAYDGGAYIQTKLSSGTSANELRVWRSCVWVGM
                     PMLASGFTFSDPRNIPTEARVKLRVKRMYEAYSTNNAEYLPDTTTATNGWRPKYRFSL
                     DNLATQTYLTTDEIKDSILSLINIVPNPYYAVSDYETGRLDTRVKVTNLPERATVRIY
                     NVSGQLIRTYTKDSPLTSLDWDLKNQAGIPIASGIYIVHVEVPEVGEKILKWFGAMRP
                     PDLQNF"
     gene            complement(4916..8662)
                     /locus_tag="PK610_02275"
     CDS             complement(4916..8662)
                     /locus_tag="PK610_02275"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRJ34778.1"
                     /translation="MLRKLSLILVFAFGSVVSYGQGQGTLQGKIKDKATGEPLAFVNL
                     VIEQNGVRQGGTSSDFDGNYKVASITPGTYTVKAMYVGYNPYVISGVVVSSNKITFLD
                     IQMESGDVTLEEVTVIEYAVPLIDKDGGASGATVTRDDIARMPGRSATSIAQTVGGVF
                     SQEGSGDLSIRGSRSDASYYFIDGIKVRGSTGLPKAAIEEVSVITGGLPANYGDATGG
                     VISITTRGASKDFFGGIDYLTSGFHFGGNSPYTGKNVGLDRYAYNLIEGSLSGPLWVK
                     KDSTGKKMKPILGFFASVNYTNIHDTRPFFTDQYVIKEDKREELLNAPLRPTGSGFGT
                     FYNTDFLRSSDFQKVRYRQNAQNRGFTGSGKIDVNLGPLINLTFGGSFDYNANNAYDY
                     NNMLFNTDNNGQIANTTWRIYGRFTQRFASAQGKEGEKSSSVIKNAYYSVMVDYSKFK
                     QTVQDANHKDRLFNYGYVGQFTTYRERSYEFGMYNGSPARIHNGFRDTLVTFSPYYLD
                     PETQEQININPDLAAITNQYYQLYTDPVGNYQNLVQIQSANALRNGDQPQSVYNLWTN
                     IGQQYNSYGQTNNSQFRITASGNADIGNHAVSLGFEYEQRDDRAFNVSPIGLWTIGRQ
                     LANGHTAELDVNNPSLTYFGSVLSVDYEPLNSSPGEYNNFDDIESQSFFDYNIRKNLG
                     LNTDGVDFLDIDAYNPAFFQLGWFSANELLNNGNNYVSYYGYSYSGERQTERVSFEDF
                     FTARDEFGNKTRPIAPFQPIYVAGYIMDKFAFDDLIFNVGLRVDRFDANQNVLQDRYL
                     LYQAKTVKEVNAQNEFGADHPTNMGDDYVVYVNTVENPTAITGYRDGRNWYNSQGEQV
                     ADPTVLFGPNGIAPYLVNPNASKEKDSEIPKAFRDYKPQINVMPRVAFSFPISDEALF
                     FAHYDVLTKRPTTGNRLDILDYYFMDVRNVVVNNPDLMPEKTIDYELGYQQVLTKSSS
                     LKISAFYRELRNQVALINVTGAYPRTYRTYGNLDFGTIKGLTLTYDLRRTGNMSIRMS
                     YTLQFAEGTGSDANSALNLVNSGQPNLRTIYPFEYDQRHALTGVVDYRYGEGKHYKGP
                     KIGGKNILENTGANVVLNVGSGTPYSAQQFVTPQSFISGGSAASLAGQVNGSRKPGQF
                     RMDMQIDRNFTLKFGKEDKKKEANLNIYLQVNNVLNTRNILNVYGATGNANDDGYLSE
                     ARYQNAIAQVNSEQSYREYYAWKTNNPYNYGLPRTIRLGIKFDF"
     gene            complement(8888..9163)
                     /locus_tag="PK610_02280"
     CDS             complement(8888..9163)
                     /locus_tag="PK610_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34779.1"
                     /translation="MILILFISGNTGLVQAQEKGGGGQEPVNKHYEKKKRQREKQNEK
                     AKKDEQKRHEQIQDKKTRKRMKKNRKKAERLKKNKREPFFQRLFRKK"
     gene            9220..9642
                     /locus_tag="PK610_02285"
     CDS             9220..9642
                     /locus_tag="PK610_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34780.1"
                     /translation="MKKSALFFTFLACFLFSCRKDDDRIPDVLVDISVNINNPGYFNL
                     QAIGGWMYFEGGSRGILVYRRSQDEFMAYDRHCPWEPANPCGKITVDTSSNIIARDAC
                     CGSAFVMTDGSVSQGPASRPLKQYLVYWDGLSILRIYN"
     gene            complement(9699..>10406)
                     /locus_tag="PK610_02290"
     CDS             complement(9699..>10406)
                     /locus_tag="PK610_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRJ34781.1"
                     /translation="SLVDAIRRATDLMLAGKVAVVAGYGDVGKGSAQSLSSQGVRVIV
                     TEIDPICALQAAMDGYQVLKMDTAVKIADIIVTATGNKNIVCDRHFKAMKHNAIVCNI
                     GHFDNEIDMAWLNSNYGKTKDVIKPQVDKYTLDGKDIIVLAEGRLVNLGCATGHPSFV
                     MSNSFTNQTLAQLELWTNSKAYENKVYTLPKHLDEKVARLHLAKIGVELETLSKEQAD
                     YIGVKVEGPFKTDQYRY"
BASE COUNT         2900 a         2301 c         2141 g         3064 t
ORIGIN      
        1 gagccgaacg ttgttctgtg gtcatttgca taccgttact tagcgtaacc aggtacgaaa
       61 atccatcacc tgaatacttc attggaggac caacattttt aaggtttatt ccaaacttaa
      121 tctgatcctt ttctcctgtc acgtaacgaa taccggcatc aaaacaaacg cctgagcctt
      181 ttacgttagc aatagactca gaaagagctt taacggtaag tccaccataa atcgaattgg
      241 agaattcttt cgcgtaagaa aacccaatat tgaagtagcg cggtgaaaaa gtacctattc
      301 ctccttcagg gatatccaca gttgtaatgg gtatctctcc aaaggtcata gaattgaagg
      361 ttaaaccaat tacacttgtt tcacctaccc gctgagccaa acctgcctgg ttcatacgaa
      421 ttccggaacc ggtcaaccaa ttgctgctga caaaattgac ttctgtcttt tttgtcatag
      481 ccaaacctgc aacattcata aatgttgctt cgattccggt tacactcgaa atacctgcgc
      541 ttccccaagt ggcactgcgt gcccaaggat tgataagtag ttcagaagct cctgcactac
      601 caacgcggtc ttcgttccct gcgtttgcta ctgttagaga acccagtcct gtaacaaaag
      661 ctattgctgt tatgatggtg tttttcatgt tcatctgctc attagagata attcttgttt
      721 cgatcgcatc agaagttttg aagatccggt ggacgcattg ctccaaacca tttcaaaatc
      781 ttttcaccaa cttcaggtac ttcaacatgc acaatgtaaa taccactggc gattggtatg
      841 cctgcctgat ttttcagatc ccagtcaagt gatgtaagcg gagaatcctt agtgtaggtt
      901 ctgatcaatt gaccagatac attgtaaatc cttacagttg cacgctcagg aagattggta
      961 accttaacac gtgtatccaa tcgaccggtt tcataatcac ttacagcata gtaaggatta
     1021 ggaacaatgt tgatcaggct taggatggag tccttaatct catcagtggt taggtaggtt
     1081 tgtgttgcca ggttatccaa tgagaaacgg tacttgggtc tccatccatt ggttgctgta
     1141 gtagtatcag gtagatattc agcattatta gttgaatacg cctcatacat tctctttaca
     1201 cgcagtttca cacgtgcttc agttgggata ttacgtgggt cactaaatgt aaatccggaa
     1261 gcaagcattg gcatacctac ccatacgcaa gatctccaaa cacgtaattc atttgcagaa
     1321 gttcctgatg aaagctttgt ctgaatatat gctccaccgt catacgccgg catacggtca
     1381 gcaaatggaa tttcacgatc ttggttagta aacacatata catagtgttt tccaccaaaa
     1441 atcggatcac ccaagttaga atacaaacga ttggttggat tgaacttcat gtcacggccg
     1501 ttatcaacac caagccagct atcttctcca aacgccatgt tcaaacgctc gccggtggca
     1561 acatcaattg cataaccggg gaaccatccc ataccggtac cggtaccatc gggatttccg
     1621 aatttatcta ctgactgagc tgcacgcatt ttttgtttcg tacgaccacc ttgtgacaag
     1681 gcagcagatt cctgcatctc gagaaccgga caacgtgtcc attttgacat atcggaagta
     1741 aagacaatgt caacgttcgg caaagtatgc aatgaagccg cagaaccaag tgttccaact
     1801 gtacctgcat accccgaacc tactgctgtt gctgcaggga attcaactcc acctgaatta
     1861 atggaagacg cacataagcg ataaggcccc caggttcccc cggcaagatt ttcatatgat
     1921 tcagtattat ccaaaccttg atagtcgttg taattacatg ggtcattctg tgggtcagga
     1981 tatgccccag catcacaagc cacatattgg gtacctgcac gtaaccagtt ttgtggtcca
     2041 ctaccctcac catcaggaac acctaccaac caacgctttg aagagtcagc aaagaaaatt
     2101 tccccggaaa taggctccgt gtactgctga cttccactaa tggtggtgta tttgtattgc
     2161 tcaattgcaa ttgaaattcc tagttcagga attaattgtt cgttattgat tcttatcgtg
     2221 gaggaagaac taacaacaac atcatttatc gcatacgtag ttccgttgat tgtcattgcc
     2281 tgtgcagctt tcaatctcca gttacttgca accagaccac ctgtagcatc aggaaggaat
     2341 tccagaatga aacttccagg ttttacatta agtgggtcaa tcactttcac actgatcgga
     2401 ccacggccag cctggtaggt tggagtagcc attttgaaat tctgaacaat gaagttttca
     2461 gactcagctg tcagatcaag gaataatgaa ccgttttgtg ctccttcaat tcttgtgatt
     2521 tcaggtccat ctccgtattt ggcaacctga tttgttcctg ctgtttcagg aacgggaatg
     2581 tgaggaattg ctgataccgc acgaatagct ccggttactg atttacgact ctcaatgtaa
     2641 ggcttcttct gtccatcaag ttttgttgca tcgtaagggt caaacgtttt gtagttatta
     2701 taggcataag caacagccat ataatagtag gtcttgtggt ttacaagacg tttctgagta
     2761 aaggcatcgt tttcaaggta aagcgaatgg aaaattccct tgtcagcacc ctcaaccatc
     2821 atagttggaa catcataatt gagatcttca tccttaatga aattcacaag gcgacctact
     2881 ccatctttaa tatcacactg tgctacaata cgcgctttat caggatctgt cagatcagaa
     2941 gatgaaacag tagcatcttt caattgatag attttgtatc cctggaagcg ataagacgac
     3001 aaactatctc ttttagcatc tgtatatgtt gcagtagagt ctccattttc aagcacaaat
     3061 ggcaatacga taaatggatc cttctccaca taggcctcgc ctacattgtt agagctattg
     3121 ggattggtaa gatataaaac aacacctctg tccaattcct gaattgtcag gtcaggtgca
     3181 tcaggtgcat ctaggatctt aaagcaattg tcaaaaagcg cctgagcctt atcatcagca
     3241 agacgaagtt tatctaccga agcatatgaa tttccggttg ttgcacgtgc ataaactaca
     3301 ccaacagtaa tgttattctt ttgtccgggt gttaaagtaa atggtccggc agactgcata
     3361 aagcgacggt cagcaggagt atttccctcg ttaaactctg tccatgaact tgcattagca
     3421 actggcactc ctaatgttcc gaataataat ggatccgaat ttccggggaa catgaaatca
     3481 caacgaacta agccattggc ctgtgcacct gcagacgatt ggtgagctgt accaccaaaa
     3541 tagaatggtg taccatctaa ccaaatacct tgaagatagc ggtagaaatc atttgcgtta
     3601 ttaggttctt tctgagcatt tacatttgtc tggtcagaac ggaagtaata aaggaattta
     3661 cgcataccaa aacgctcgtt gtcaactacg ccgtctccat aaccgatacc gatacctttg
     3721 taaggaatac caagactgtc gatagcatca gaaatatttg tagtaagcgg gttgtcgata
     3781 ttatcattat cctgaaaagg accctggaaa aagtcaatac caacagccgg gggattagcc
     3841 ccataacctg attgtccctg acctgcatca tcgaatgcat ctccattata ggcgtaaccc
     3901 aatccgcgtt gaacatcgca acctacaaaa tcgtcttcag agaaccctac gtcggggtca
     3961 caccactgac caaaataagt attgaataat gtttgtgtac cacggttgta aagttcgtag
     4021 ttgtagaatg tcatgtcatt tacctcatcg ttggttgaga aagcaaatgc ctgagcacga
     4081 atttccatac caattggatc ggcaccggtt tctgtgtgaa tgtttccttt atcgttaaac
     4141 acccaccaga aattctggtc accataaagc gttacagtac ggtcgtttcc acaagcaagt
     4201 tccttcttga tatcgtacca aggatagtca ccatcaccag gattgtaaac tccgtcgtta
     4261 ttaacgtctt tataaggcgc aagattgaaa tcgtatccgg cagcagaatt gtttccaggc
     4321 cattcgagaa ttgaacgcgg cacagtatac ccggggaata aatcgccgca atttggtgca
     4381 ccggactgaa tacattcagt ataggcaaca aaggttacga tgtcctgacg tagggttaca
     4441 aagaacttgt cgtaatacaa gcaagtagcc tgatcaactt ctgctgtacc atctgttaaa
     4501 agcgggccgg gccaaaaatc attaccttga cggaatgtta ctgctgccac tttaagctgg
     4561 tcgttaaagt cttttccgcc caaccaaagt gcacccgcaa acattactcg caaccgtgac
     4621 cctttgggca cttcgtaagc agagaagcct tgagcacgat cctgccacat atttccacct
     4681 gtttcaatga tggcgctcac gttattgaac tccagttttg ttaaggcggt tgcaggagca
     4741 caacctgcag ccctttgttg catgccggta gttgatccgc ttgcatttcg caatgcaggg
     4801 tcaatttctg cgcgaagctg gctggttgcc aacagaccca gaacaagccc tgttccaagt
     4861 cgcattatcc ctttcctatt catagtcgat cgcttaatca ttcgttgaat catcattaga
     4921 agtcgaactt aatccctaaa cgaattgttc tgggtaagcc gtaattgtaa ggattgtttg
     4981 ttttccatgc atagtactct ctgtaagact gttcagagtt tacctgtgcg atagcattct
     5041 ggtaacgtgc ttctgataaa tatccatcgt cgttggcatt accggttgct ccgtacacat
     5101 ttagaatatt tcgtgtattc aagacattgt taacctgcaa atagatattc aggtttgctt
     5161 ctttcttctt atcctcttta ccaaacttca atgtgaagtt acggtcaatc tgcatgtcca
     5221 tacggaactg accaggcttt cttgaaccgt taacctgacc tgcaagtgat gctgcggatc
     5281 cgccactgat aaacgattgt ggggttacaa actgttgagc actgtagggc gttcctgaac
     5341 caacgttcaa tacaacgttt gctccggtgt tttcaagaat gtttttcccg ccaatttttg
     5401 ggcctttgta atgttttcct tctccataac ggtaatcaac aactcctgta agagcgtgac
     5461 gctgatcata ttcgaaagga taaatagttc taaggttagg ttgccctgaa tttacaagat
     5521 tcaatgctga attggcatca gaacctgttc cttctgcgaa ctgcaatgtg taggacatac
     5581 gaattgacat atttccagtt ctgcgcaagt cataagtaag tgtaagacct ttaattgtac
     5641 caaagtcaag gttaccatag gtacggtaag tacgagggta agcacctgtt acattgatca
     5701 aggcaacctg gttacgaagc tcacggtaga aagcagaaat tttcaatgaa gaactcttgg
     5761 tgagtacttg ctgataaccc aactcatagt cgattgtctt ttcaggcatc agatcagggt
     5821 tgtttacaac cacgttgcga acatccatga aataataatc caaaatatcc aaacgatttc
     5881 cagtagttgg acgcttagtc aatacatcat agtgtgcaaa gaataacgcc tcgtcactga
     5941 tcgggaacga gaaagcaaca cgcggcatca cattaatttg tggcttgtag tcgcggaatg
     6001 cttttggaat ttcagagtct ttctccttgg atgcatttgg attaaccagg tatggtgcaa
     6061 ttccattagg accaaagagt actgttgggt cagcaacctg ctccccttga gagttgtacc
     6121 agtttcttcc atcacggtaa ccggtaatag ctgttgggtt ctcaactgta tttacataaa
     6181 ctacataatc atctcccatg ttagtagggt gatcggcacc aaactcattt tgtgcattta
     6241 cttcttttac cgtttttgcc tgatacaaca aatagcgatc ctgaagtacg ttttggtttg
     6301 cgtcaaaacg gtcaacgcga agtcctacgt taaagatcaa atcgtcaaac gcaaacttat
     6361 ccatgatata tcctgccaca taaataggct ggaacggtgc aattggacgc gttttatttc
     6421 cgaattcatc acgagctgtg aagaagtctt caaaagaaac tcgctctgtt tgtctctctc
     6481 ctgaataaga ataaccgtaa taagaaacgt aattattacc attattaaga agttcattgg
     6541 cagagaacca tcccaactgg aagaatgcag gattataggc atcgatatct aaaaagtcca
     6601 ctccatcagt attcaatccc agattctttc tgatgttgta atcgaaaaac gattgtgatt
     6661 cgatatcgtc aaaattattg tactctccgg gtgaggaatt gagcggctca tagtctacag
     6721 ataacaccga accaaagtag gtgagcgaag gattattaac atctaactca gcggtatgcc
     6781 cgtttgccag ctgacggcca atcgtccaaa gtccaatcgg cgaaacattg aatgcgcggt
     6841 catcacgttg ctcatattca aaaccaagtg atacagcatg gtttccgata tcggcgtttc
     6901 ctgatgccgt aatacgaaat tgtgaattat tggtttgacc ataactgttg tactgttggc
     6961 caatgtttgt ccaaaggtta taaaccgatt gtggctgatc accgttacgc aatgcgtttg
     7021 cagactgtat ctgaacaagg ttctgataat ttccaaccgg atccgtataa agctgataat
     7081 actgatttgt tatggcagcc aggtctgggt taatattgat ttgttcctgt gtttcgggat
     7141 ctaggtaata aggggagaag gtaaccaaag tatcgcggaa accattatgg attcttgctg
     7201 gtgaaccatt gtacattccg aattcatatg aacgctcacg atatgtagtg aactgaccta
     7261 catacccgta gttaaacaag cggtctttgt gattcgcatc ctgaactgtt tgtttgaatt
     7321 tagaataatc gaccattaca gagtaatagg cattcttaat aactgaagag cttttttcac
     7381 cttcttttcc ttgagcagaa gcaaagcgct gggtaaagcg gccgtaaata cgccaggtag
     7441 tgtttgcaat ctgaccattg ttatcggtat tgaataacat attgttatag tcatatgcat
     7501 tattggcatt gtaatcgaaa gaacctccaa atgtgaggtt aatgagcggg cctaaattca
     7561 catcgatttt acccgaacct gtgaatccac gattttgggc gttctgacgg taacgaactt
     7621 tctggaagtc ggaagagcga aggaagtcgg tgttgtaaaa tgttccgaat cctgatccgg
     7681 taggacgaag tggtgcattc agtaattctt ctcttttgtc ctccttgatc acgtactgat
     7741 cagtaaagaa aggacgggta tcgtgaatgt ttgtatagtt tacagaagca aagaaaccaa
     7801 gaatgggctt catttttttg cctgttgaat cctttttaac ccatagcgga ccggaaagtg
     7861 atccttcgat aaggttgtag gcatagcggt caagaccaac atttttaccg gtatatggac
     7921 tatttcctcc aaaatggaaa cctgaagtca ggtaatcgat acctccgaag aaatcttttg
     7981 aggcaccgcg tgtagtaatg gagattaccc caccagtagc atccccgtag ttagcaggaa
     8041 gtccacctgt aattacagat acctcttcaa ttgcagcttt tggaagaccg gtagaaccac
     8101 gaactttgat accatcaatg aagtaatagc tggcatccga acgtgaacca cggatcgaaa
     8161 gatcaccact accttcctgg gagaatacac ctccaacagt ttgtgcaatg gaagttgcag
     8221 aacgacctgg catacgtgca atatcatcac gcgttacagt tgctcctgaa gcaccaccat
     8281 ctttatcgat aaggggaacg gcatattcga ttacagtaac ctcctcaagc gtaacatcac
     8341 cggattccat ctgtatatcg aggaaggtaa ttttgttaga actaacaaca acacccgaaa
     8401 ttacataggg gttataaccc acatacatgg ctttaaccgt ataggtaccg ggagttatgg
     8461 aggcaacctt atagttaccg tcaaaatcgg aggaggtacc tccttgtcga acaccattct
     8521 gttcgatgac caggttcaca aatgccagcg gctcaccggt ggctttgtcc ttgatttttc
     8581 cttgcagggt tccttggcct tgtccgtagg aaactacgga accaaaagcg aacaccagta
     8641 tcaagctcag ttttctaagc atacgttcaa gttgttattc gtttatcgca acaaaggccg
     8701 taaatataga aattatatat cccgcagcaa gccccattta agcaacggcc gatttccatg
     8761 tattatcgtt ttttttcgga aatcttccct tgttgggatc ttgattgttc aaatttactc
     8821 ctgtttacgg caggtttcag gttttgaaga ttcaaaaaag gtcaattcta tacaagtctg
     8881 aaccgcatta tttttttctg aacaaccgtt gaaaaaaggg ttctcgcttg ttttttttca
     8941 atcgttcggc ctttttcctg ttctttttca tgcgtttacg ggttttttta tcctggattt
     9001 gctcgtgccg tttttgttca tcttttttgg ctttttcgtt ctgcttttca cgttgccttt
     9061 tctttttctc gtaatgcttg tttaccggtt cttgtccgcc accgcccttt tcctgtgcct
     9121 gcaccagacc tgtgtttcct gaaatgaaca ggattaagat cattctgaga ataaatcttg
     9181 tcatcttgcg tatctttata gtatgttgtt tcgaatataa tgaaaaaatc agcgctcttt
     9241 ttcacctttc tggcttgctt tctgttctcc tgcagaaagg atgatgaccg tattccggat
     9301 gtattggtgg atatttcggt aaacatcaat aacccgggat attttaatct acaggctatc
     9361 ggcggatgga tgtattttga aggtggatca agaggaattc tggtatacag aaggagtcag
     9421 gacgaattca tggcttacga caggcattgt ccctgggaac ctgctaatcc ttgtggtaag
     9481 attactgttg acacaagcag caacattatt gcacgtgatg cttgttgcgg atctgcgttt
     9541 gttatgactg atggcagcgt atcacaaggt ccagcctcgc gaccgctaaa acaatatctg
     9601 gtttactggg acggactttc tatccttagg atttacaact aatagtattg aaaataaaaa
     9661 agccccttcg agaggggctt tttatttttg tggaccgatt aataacggta ttgatctgtt
     9721 ttgaaaggcc cttcaacctt aacgccgata taatcagctt gttcttttga aagcgtttcg
     9781 agctcaacac caatttttgc aaggtggagt cgtgcaactt tttcatcaag gtgtttaggc
     9841 aaagtataaa ctttgttttc gtacgctttt gaatttgtcc aaagctccaa ttgcgccaat
     9901 gtttggttgg taaaggagtt agacattaca aatgaagggt gccctgttgc gcatccaaga
     9961 ttcaccaaac gaccttctgc caatacgatg atatctttcc cgtccaaagt gtacttatcc
    10021 acctgaggtt taataacgtc tttggttttg ccgtaattgc tgttcagcca agccatgtcg
    10081 atttcattgt cgaagtgacc gatgttacaa acaatcgcat tgtgcttcat tgccttgaag
    10141 tgacgatcgc aaacgatgtt tttgtttcct gtagcggtaa caataatatc agcaattttt
    10201 actgcggtat ccattttcaa aacctggtaa ccatccattg cagcctgaag cgcacaaata
    10261 ggatcgatct cagtaacaat aacacgaaca ccttgtgaac tgagtgattg tgcagaacct
    10321 tttcctacgt cgccataacc tgcaactact gcaaccttac cggcaagcat taaatcagtt
    10381 gcacggcgaa ttgcgtcaac caatga
//
LOCUS       DAOMYG010000040        10393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2136_length_10393_cov_11.646934, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000040 DAOMYG010000000
VERSION     DAOMYG010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10393
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2136_length_10393_cov_11.646934"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..5622
                     /locus_tag="PK610_02295"
     CDS             28..5622
                     /locus_tag="PK610_02295"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ34782.1"
                     /translation="MMQDPNANFYDVVAEFESYWSGRTIEKGKGYKVFRRWQNFMEPR
                     VYPKGDMKQAHRAYQEFQQWLTTNPSILAGERAGNWTIMGPSTTPTNGGNGRINFVRF
                     HPTNTNIIYAGSPAGGLWITTNGGTSWNTNTDQLSVIGCTDIAIDPATPATMYLATGD
                     GDANDTYSIGILKSTDGGNTWNTTGLTWTVNQGRRISRLLIHPTNTQILLAATSNGIY
                     RTTNGGGAWTQVRTGNFKDMEYKPGDPNTVYACGTTFWKSTDGGATWTQVTSGVPSAN
                     VGRLAIATTAANSTYVYMIAARADDNGLLGVYRSTDNGTTFTTRSTTPNYLGYNSNGG
                     DAGGQGWYDLAIAASPTNADVVIVGGINIWRSTNGGTNFTINGHWTGSGAPYVHADIH
                     DLIFLPGNGTTYYAGCDGGVFRTANSGTGWSDISANLAIAQQYKIGLSASNANLLLAG
                     HQDNGTNRYNGTTWAQVYGGDGMDCFIDRTNNNTMYASYVYGDFKRSTNGGGAWTTIT
                     TGLTGNGAWVTPWKQDPTTANTLYAGYQQVFRSTNQGTSWTSISSFTNTNTIVELKIA
                     PSNNQVIYVVKANAVFKTTNGGTSWTTITGTLPVASAAITNIDISTTDPNRVYVTFSG
                     YSAANKVFMSTDGGTTWTNYSTGLPNLPVNCIVYQNAANEAVYCGTDVGVYYRDASQP
                     SWIPFFDGLPNVIVNDLEIYYPTSKIRAATFGRGTWQSDLYSPGSQLPTADFTANRQS
                     ICVGQTVTFTDLTSGTPTAWNWSFAGGTPATSTQQNPTVTYNTAGTYNVTLTATNANG
                     TNSVTKTAYITVSNQQALPLTEGFETSFVPAGWYNMDAMGDGTVWQATTSASGFSTST
                     ACSVFDNYNNDVSGTRDELWTPKYNFSGYTSITMTFDVAYARYSATYSDTLAVMISTD
                     CGVTWTQVFMKGGTTLATNGGANVTTLFIPTNTQWRTETLNLNTFIGSQNVVIAFQNR
                     GRYGQPIFLDNINISGVMPSLPTASFTGSPTSVCPGQTVNFTNTSTGATTYNWSFPGG
                     TPATSTATNPSVTYATPGTYSVTLTASNGAGTTTTTQTNYITVNANPTATAGSNTPVC
                     TGNSINLTANTVAGASYVWSGPATFSSSLEDPTRPNATTAMAGTYTVTITNASGCTAS
                     STTAVVVNNTPTATATSNSPVCAGQTLTLSTPAVTGATYAWSGPGGYTSTTRNPSRTN
                     ATTAMSGTYTVTVTANGCATTSTVSVTVNTAPTATAGSNTPVCSGNAINLTANTVASA
                     TYAWTGPNSFTSTLEDPSIASSTTAMSGTYTVTITNGGCSATSTVAVTVNATPTATAS
                     SNSPICATQTLTLSTPAVTGATYAWSGPGGYTSTTRNPSRTNATTAMSGTYTVTVTAN
                     GCSSTSTVSVAINNTPTATASSNGPVCAGQAIHLNAGNVTGATYAWTGPNGFTSTQQN
                     PGIPSSTAAMAGTYTVTVNNGCSATATTSVTVNPSPVANASNSGAVCEGGTVNLSTTT
                     AGGASYAWSGPGGFTSTSQNPTITGAGISDGGSYTVTVTAAGCSNTSSTVVTINSNPI
                     VGTSGDVAVCSGNSTTLTASGGTSYSWTPTATLSNPSIMNPVATPSGNTTYSVTVTDG
                     NGCTGTAQIVVTVDSQITVSVSGDVSICEGESTPLTANGGSTYSWSPVTGLSSSTGAA
                     INASPVSTTTYTVTGSTGACSNTASVTVTIIPAPASPVITQVGNDLESTPALTYQWLL
                     NGTAISGANGQTYTPTQDGDYDVIITDASGCESMSSSSFTYSSGGSISVEENNSTYLV
                     TLLPNPATGEFYIEITSATDGVFPMEMMNSVGQISLKKDLVLQSGILSRTMINVPELE
                     RGVYIIRILVENEIITRRIILM"
     gene            complement(5687..6850)
                     /locus_tag="PK610_02300"
     CDS             complement(5687..6850)
                     /locus_tag="PK610_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRJ34783.1"
                     /translation="MRRIYPLTLIILLFSSQSVFGQQIRIGLFRNDFPQSVMLTSIGN
                     YKVFADSSMLGILPSGTGIRLILDSSGVRMNSIDRDWGAYSQLLLVPNDSFAVMKIKG
                     IAPVTKERSFGGKISIDQDAASLRIINHLDLEEYLAGVVEAEAGYGHHIEFYKAQAII
                     SRTYALAHLYKHLPEGFHLCDQVHCQAYKGKGRSNADVLTAAKATRGIVIVDDSLHFI
                     TAAFHSNCGGQTANSEHVWTKPLPYLRSVKDNYCTSQPNAYWTREISIAEWKNYLKRY
                     NFPVHDSSCITYCTTFKQPYRMYCLSSCNKTIPFKNIRADFGLKSAFFSLERVNEHTV
                     VLRGRGFGHGVGLCQEGAMKMAKLGFDFKIILKHYFTGVHIIDIREKDTFAEE"
     gene            complement(6884..7999)
                     /gene="lpxB"
                     /locus_tag="PK610_02305"
     CDS             complement(6884..7999)
                     /gene="lpxB"
                     /locus_tag="PK610_02305"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRJ34784.1"
                     /translation="MKYYIIAGEASGDLHGSNLLRELKNQDPSAEFRAWGGDLMAAQG
                     ADVVKHYQELAFMGFTEVLMNIRTILKNMKRCEEDIVQWKPDVVILIDYPGFNLRIAK
                     KMKQHGIRVFYYISPQVWAWKQSRVKIIREVVDRMFVILPFEKSFYEKWNYEVDFVGH
                     PLLDAIQNLPSLEGEHTPPWKKEGKPVIALLPGSRKQEISKMLPLMLSVQNHFPEYRF
                     IIAAAPSMPESFYRSVMGDLNGELVFGRTYDVIRSADAALVTSGTATLETALLNTPEV
                     VCYKGGKISYHIARRLIKVKFISLVNLIMDREVVKELIQDDLTTANLVNELKGLLPTG
                     IKREGVLADYAELRNALGNAGASAKTAGLMLKTLKGA"
     gene            complement(7999..8295)
                     /locus_tag="PK610_02310"
     CDS             complement(7999..8295)
                     /locus_tag="PK610_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34785.1"
                     /translation="MMQRFDKAIYGFIAGSVLPFLGYSVGKFIIYRSGSWASYWSYFK
                     RPGDTQTQIFTFCMLPTLFLFYFVFFQWKLEQASKGLVAISLVAVTIFMAFKFL"
     gene            complement(8295..9062)
                     /gene="surE"
                     /locus_tag="PK610_02315"
     CDS             complement(8295..9062)
                     /gene="surE"
                     /locus_tag="PK610_02315"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRJ34786.1"
                     /translation="MKKKKPVILVTNDDGITAPGIRALVEAVKGFGTILIVSPDSPQS
                     GMGHAITVNEPLRLYPSKEYGKLESYACSGTPVDCVKLGIYEILKYSPDLIVSGINHG
                     SNVSTNVLYSGTMSAAVEGALEGIPSIGFSLTDYSMTADFSAAQKVVKSIVKEVLEKG
                     MPEGICFNVNIPKLPYSKIKGVKVCRQAHAFWEDKFDNRKDPLGKPYYWLTGEFKSYD
                     GGKDTDLWALENNYVSLVPVQYDMTAHQVLGKLNYSF"
     gene            9197..10165
                     /locus_tag="PK610_02320"
     CDS             9197..10165
                     /locus_tag="PK610_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ34787.1"
                     /translation="MSVNYKSDVEAVNAFVEKYKILHKEIGKVIVGQEQVVDEVLISI
                     FSKGHCLLVGVPGLAKTLLVNTIADALGLSFNRIQFTPDLMPSDIIGSEILDETRNFK
                     FIKGPLFANIILADEINRTPPKTQSALLEAMQEKAITASGKRYKLDEPFFVLATQNPI
                     EQEGTYPLPEAQLDRFMFNIWVDYPKFEEELAIVRNTTSDMKVKLEKVLSSEEILYFQ
                     DLIRRIPVADNVLEYAVKLSSKTRPNTPGATDIVNKYLSWGAGPRASQYLVVGAKCHA
                     AVKGKYSPDIEDVQAVAMPILRHRIVRNYMAEAEGYSIEKIIKSLL"
BASE COUNT         3136 a         2402 c         2143 g         2712 t
ORIGIN      
        1 ggattgttct cgcaacagta cgtgcagatg atgcaagatc cgaatgctaa tttttacgat
       61 gtcgttgccg aattcgaaag ttattggtcg ggcagaacga tagagaaagg taaaggatac
      121 aaagtttttc gtcgctggca gaatttcatg gaaccccgtg tttatccaaa gggagacatg
      181 aaacaggctc atcgggccta tcaggagttt cagcaatggt tgaccaccaa tccttctatt
      241 cttgcaggag aaagagctgg taactggacc attatggggc cctccaccac tccaacaaat
      301 ggcggaaatg gccgaatcaa tttcgtcagg tttcacccta ccaatacaaa catcatttat
      361 gcaggatcac ctgcaggcgg attgtggata accaccaatg gaggtacatc gtggaatacc
      421 aataccgatc agttatctgt tattggttgt acagatattg cgatagatcc ggcaacacct
      481 gccaccatgt atctggcaac aggcgacgga gatgcaaatg acacctattc gattggaatt
      541 ttaaaatcaa cagatggtgg taatacgtgg aataccacag gactcacctg gaccgtcaat
      601 caaggcagaa gaatttcacg gttattgatt catccaacga atacccaaat tctattggca
      661 gccacttcca atggaatata ccgaacaacc aatggcggag gcgcgtggac acaggttcgt
      721 acagggaatt tcaaggatat ggagtacaaa ccgggagatc ccaacacggt gtatgcatgt
      781 ggaaccactt tttggaaatc aacagatgga ggcgcaacct ggacacaagt tacaagtgga
      841 gtaccatcag caaacgtagg tcgattggcg attgcaacaa cagcagcgaa ttcaacctat
      901 gtgtacatga tcgctgcacg ggcagatgac aatggattat tgggtgttta tcgttcaaca
      961 gataacggaa caacgtttac cacaagatca accacaccaa attaccttgg atacaactca
     1021 aacggaggag atgcaggcgg ccagggttgg tatgatttgg caattgcggc ttctcccacc
     1081 aacgcagatg ttgtaattgt cggaggaatc aacatctggc gatctaccaa tggcggaacg
     1141 aatttcacca ttaacggaca ttggacagga tcaggcgcac cttatgttca tgctgatatt
     1201 catgatctga ttttccttcc cggtaatgga acaacctatt acgcaggttg cgacggcggc
     1261 gtttttcgca ctgcaaattc aggaacaggc tggtcggata tttctgccaa tcttgcaatt
     1321 gcacaacaat ataaaatcgg actttcagca agtaatgcca acctgttatt ggcaggtcac
     1381 caggataacg gaaccaaccg ttacaacgga acaacctggg cacaagtgta tggtggagac
     1441 ggaatggatt gtttcattga ccgcacgaac aacaacacca tgtatgcttc ttatgtatat
     1501 ggagatttca aacgatcaac caatggtgga ggtgcatgga caaccatcac aaccggatta
     1561 accggaaacg gcgcctgggt aacaccatgg aaacaagatc caacaactgc gaatactctt
     1621 tatgcaggtt atcagcaagt attccgatct accaatcagg gaacttcatg gacgtccatt
     1681 tcaagtttta ccaacaccaa tacgattgta gaacttaaga tcgcaccttc caacaatcaa
     1741 gtcatttacg tggttaaagc gaatgccgtt ttcaaaacca ctaatggagg aacaagctgg
     1801 acaaccatca caggaacatt accggttgca agtgctgcga ttacaaatat tgatatcagt
     1861 acaaccgatc cgaacagggt ttatgttacg ttttccggtt actctgcagc gaacaaagtt
     1921 tttatgtcga ccgacggagg aacaacctgg acaaactatt ctaccggatt acccaatctt
     1981 cctgtaaact gtatcgttta tcaaaacgcg gcaaatgaag cggtttattg cggaacagat
     2041 gttggcgttt attacagaga tgcgagtcaa ccttcatgga ttccattctt tgacggatta
     2101 ccaaacgtga ttgtaaatga tcttgagatc tattatccaa cttcaaaaat ccgtgcagct
     2161 actttcggaa gagggacatg gcaatccgat ttatattcac ccggaagtca gctaccgact
     2221 gctgatttta cagccaaccg tcagagtatt tgtgttggtc agaccgtaac atttaccgat
     2281 ctcacatccg gaaccccaac agcctggaac tggtcatttg ccggaggaac acctgcaaca
     2341 tctacacagc aaaacccaac agtaacctat aatactgcag gaacatacaa tgttaccctt
     2401 acagccacca atgcaaatgg tacaaatagt gttacaaaaa cagcctacat cactgtaagc
     2461 aatcaacaag ccttgccctt aacagaagga tttgaaactt cttttgttcc tgcaggctgg
     2521 tacaatatgg atgcaatggg cgatggaacc gtatggcaag cgaccacctc agccagcggc
     2581 tttagcacat caactgcatg ttctgttttt gataattata ataatgatgt atccggaacg
     2641 cgggatgaat tgtggacacc caaatacaat ttcagtggtt ataccagtat taccatgaca
     2701 tttgatgtag cctacgcacg atacagcgcc acttattccg atacacttgc cgtaatgatt
     2761 tcgacagact gcggggttac atggactcaa gtgttcatga agggaggcac tacattggca
     2821 acaaatggag gagcaaatgt tacaacactt ttcattccaa ccaacaccca atggagaact
     2881 gaaacactga acctaaatac ctttatcgga tcacagaatg ttgttattgc attccaaaac
     2941 agaggaaggt atggtcagcc gatattcctc gataacatca atatttcagg cgtcatgccc
     3001 tccttaccta cagcaagttt tacaggaagt ccaacatctg tttgtccggg ccaaactgta
     3061 aactttacca atacctcaac cggagcaacc acttataatt ggagtttccc cggaggaacg
     3121 cctgccacat ctacagccac gaatccgtcg gtaacctatg ccacacccgg cacctatagt
     3181 gttacactta cagcttcaaa tggtgcagga acaacaacaa ccacacaaac caattatata
     3241 actgttaatg caaatccaac agcaactgca ggaagcaata caccggtttg tacaggaaac
     3301 tccatcaatc tcacagctaa tactgtggca ggagcaagct atgtttggtc aggaccggca
     3361 accttttctt catcgctgga agatccgaca cgtcctaacg caacaactgc aatggcgggt
     3421 acttatacag ttaccattac caatgcgtca ggctgtactg cttcttcaac tactgcagtt
     3481 gtggtaaaca atactcctac tgcaacggca acgagtaatt ctccggtttg tgcaggacaa
     3541 acacttacac tgagtactcc tgcagtaaca ggagcaacct atgcttggtc aggccccggt
     3601 ggatatacat caacaacccg aaacccatcg cgcaccaatg caactactgc aatgagtgga
     3661 acctacaccg taaccgttac tgcaaatgga tgtgcaacaa cttctacggt aagtgtaacg
     3721 gttaataccg cacccactgc aacagcaggt agcaacacac ctgtttgttc cggaaatgca
     3781 atcaatttaa ccgctaacac cgtggcaagt gcaacctatg catggacagg tcctaactca
     3841 tttacctcta cgctcgaaga tccaagtatt gcttcttcta ctaccgcaat gagtggaaca
     3901 tacacggtta ccattaccaa tggagggtgt tctgcaacat ctacagttgc agtaacagta
     3961 aatgcaacac caactgcaac tgcctccagt aattcgccca tctgcgcaac acaaacgcta
     4021 actctcagca caccggccgt tacaggagca acatatgcct ggtctggccc cggtggatat
     4081 acctcaacta cacgtaatcc atcgcgcacc aatgccacaa ctgcaatgag tggaacttac
     4141 accgttactg taactgcaaa tggttgttca agtacttcaa cagttagcgt agctatcaat
     4201 aatactccaa cggcaacagc aagcagcaat gggccagttt gtgccggtca ggcgattcat
     4261 ttaaatgcag gaaatgttac aggcgcaacc tatgcctgga caggtccgaa cggatttaca
     4321 agtacccagc aaaatcctgg tattccatcc tcaacagccg ctatggccgg tacatacaca
     4381 gtaaccgtaa acaatggatg ttctgcaact gccacaacaa gtgttacagt taatccatcg
     4441 ccggttgcca atgccagtaa ttctggtgcg gtttgtgaag gtggaactgt aaacctcagt
     4501 acaaccacgg ccggtggtgc aagttatgca tggtcaggcc ccggaggatt tacatctaca
     4561 tcgcaaaacc caactatcac cggagcagga atttctgacg gaggaagcta tactgtaaca
     4621 gtaacagcag ctggatgtag caacacttca tcaacggtgg ttaccattaa cagtaatccg
     4681 atagttggta catccggcga cgtagctgta tgtagcggta acagcacaac acttacagct
     4741 tccggaggaa cttcttacag ctggactccc acagcaacat taagcaatcc atccattatg
     4801 aatccggttg caactccttc ggggaataca acctattcag ttaccgttac agatggaaac
     4861 ggatgtacag gtacagcaca aatagtagtt acagtagaca gtcaaattac tgtttcggta
     4921 agcggagacg ttagtatttg tgaaggagaa tcgacaccac taactgcaaa tggcggatca
     4981 acctatagct ggtcacctgt aaccggatta agttcctcaa caggtgcagc aatcaatgca
     5041 tcacctgttt caacaactac gtatactgta accggtagca caggtgcttg tagcaatact
     5101 gcaagcgtaa cggtaactat catacctgct cctgcttcac cggtaattac acaggttgga
     5161 aatgatcttg aatctacccc agctttaact tatcagtggt tattaaatgg aacagcaatt
     5221 tcaggagcga atgggcaaac ctacacacca acacaagatg gtgattacga tgtaatcatt
     5281 accgatgctt cagggtgtga atctatgtcg tcgagttcct ttacctactc gtcaggaggt
     5341 tccatttctg tagaagaaaa taattctact tacctggtaa cgttattacc taatcctgca
     5401 acaggagagt tttacattga gatcacttct gcaacagatg gtgttttccc tatggaaatg
     5461 atgaattcag ttggtcagat cagtcttaag aaagaccttg ttttacaatc aggcattctt
     5521 tcaagaacaa tgattaatgt tcctgaactt gaaagaggtg tatacatcat ccggattctg
     5581 gtagaaaacg aaattattac cagaagaatt attctgatgt gatcctgtta caaaccaata
     5641 aaaaagcacg gaaatctccg tgcttttttt atttgacttc atttttttac tcctcagcga
     5701 aagtgtcctt ttcgcggata tcgataatgt ggactccagt gaaatagtgc ttgagaatga
     5761 tcttaaaatc aaaaccgagt ttagccattt tcattgctcc ctcctggcac aatccaactc
     5821 catgtccgaa tcctctgccg cggagtacaa cggtatgttc atttacccgt tcaagactga
     5881 aaaaggcgga tttcagccca aaatctgcgc ggatattttt aaaaggtatg gtcttattgc
     5941 aagagctcaa acaatacatt cggtaaggct gcttgaaagt ggtgcaatac gttatacagg
     6001 aagaatcatg taccggaaaa ttatacctct tcaggtaatt tttccattca gcaatagata
     6061 tttcgcgtgt ccaataggcg ttgggctgcg atgtgcaata attatctttt acgcttctta
     6121 aataaggcaa aggttttgtc caaacgtgtt ctgaattggc tgtttgtccc ccgcagttgc
     6181 tatgaaaagc ggctgtaata aaatgtaaac tatcatccac aattacaatt ccgcgtgtag
     6241 cttttgcagc ggttagtaca tcagcattcg atcttccttt tcctttgtag gcctgacaat
     6301 gcacctgatc gcacaaatga aatccttccg gtaaatgttt gtacaaatga gcgagtgcat
     6361 acgttctgga gataattgcc tgtgccttgt aaaattcgat atggtgacca taaccggctt
     6421 ccgcttccac tacaccggca aggtattctt caaggtcgag atgattaata attcgaagtg
     6481 aagcagcatc ctgatcgatt gaaattttcc ctccaaaact tctctccttc gtaactggag
     6541 caatgccttt aattttcatt acagcaaatg aatcgttagg cacaagcagc aactgactat
     6601 acgcacccca atcgcgatcg atactgttca tccgaactcc tgaagaatcc agaattaagc
     6661 gtattccggt tcccgatgga aggataccga gcatggagga atcagcaaag actttgtaat
     6721 taccaatcga tgtaagcata accgattgtg ggaaatcatt tcggaaaagt ccgatgcgga
     6781 tctgctgacc gaaaacagat tgggatgaga aaagaagaat gattaatgta agcggataaa
     6841 tcctgcgcat gcaacaaaat tacaaagcgt aagaggtcga aatctacgcc cctttcagag
     6901 ttttcaacat gagaccggca gttttggctg acgcaccagc attgccaagt gcatttctta
     6961 attcagcata gtcggcaagg acaccctccc ttttgattcc ggtgggaagc agtcctttta
     7021 gttcgttcac cagattagcg gtagttaaat catcctgaat cagctctttc accacttccc
     7081 tatccatgat aaggttaacc agtgaaatga atttgacttt gataagtcgt cgtgcaatgt
     7141 ggtaggatat ttttccgcct ttgtagcaaa ccacttccgg agtattgagt aatgctgttt
     7201 cgagtgttgc tgttccggat gttacaagag ctgcatcggc agaccggatc acatcgtagg
     7261 tacgaccgaa aaccaattca ccatttaaat cacccattac gctgcggtaa aaactttcgg
     7321 gcatggacgg tgcagcggca atgataaaac ggtattcggg aaaatggttt tgaacggaaa
     7381 gcatcagcgg aagcattttg gaaatttcct gcttgcggct tcccggaaga agagcaatta
     7441 ccggtttacc ctcttttttc caaggcggcg tatgctcacc ctctagtgaa ggaaggttct
     7501 gaatggcatc aagaagcggg tgtcctacaa aatctacttc ataattccat ttctcataaa
     7561 atgacttttc aaaggggagg atcacaaaca tgcggtcgac aacttcgcgg ataattttaa
     7621 cgcgggattg tttccaggcc catacctgag gagaaatata gtagaacaca cgaataccgt
     7681 gctgcttcat tttttttgcg atgcgtaaat taaatccggg gtaatcgatc aaaataacca
     7741 catccggttt ccattgcaca atatcctctt cacacctttt catgtttttc aggatcgtac
     7801 ggatattcat caatacctcc gtaaagccca tgaatgcaag ctcctggtaa tgtttaacaa
     7861 catcagcgcc ctgggcagcc atcaaatcac ctccccatgc acggaattcg gcagaaggat
     7921 cctgattctt taattcacgc aataaattac taccgtgcaa atcacctgaa gcttcaccgg
     7981 ctatgatgta atacttcatc agagaaattt aaacgccatg aaaatggtaa cagctaccag
     8041 gcttatagct actaatcctt tgcttgcctg ctcaagtttc cactgaaaaa aaacaaaata
     8101 gaaaaggaat aatgtgggca gcatacaaaa tgtaaaaatc tgcgtttgtg tatcgccggg
     8161 tcgcttaaaa taactccaat acgatgccca ggaaccactg cggtaaatga tgaatttccc
     8221 aacagaatat cccaaaaacg ggaggacgga tcctgcaata aatccgtaaa tcgctttatc
     8281 aaatcgctgc atcatcagaa cgaatagttt aatttaccca atacctgatg tgcggtcatg
     8341 tcatactgta ccggaaccaa tgaaacataa ttgttctcca atgcccagag atctgtatcc
     8401 tttccaccat cgtaactttt aaactctccg gtgagccagt aataaggttt tccaagcggg
     8461 tcttttcggt tatcaaattt atcctcccaa aaagcatgag cctgacgaca aaccttaact
     8521 ccttttattt ttgaataggg aagcttgggt atatttacat taaaacaaat tccttcgggc
     8581 atcccttttt caaggacttc cttcactatg gatttaacca ccttttgagc tgcagaaaaa
     8641 tcggcggtca tggaataatc ggtcaacgaa aatccgatag aaggaatccc ttccaatgca
     8701 ccctcaaccg cagccgacat ggttcctgaa tacaacacat tcgtgctgac gttagacccg
     8761 tgattgattc cggaaacgat gagatcaggc gaatatttca gaatctcata aatccccagt
     8821 ttaacacaat ccacaggtgt tccggaacaa gcataagatt cgagtttacc gtactctttg
     8881 gagggataca accgcaatgg ttcattcacg gtaattgcat gtcccatccc cgattgagga
     8941 ctatcaggcg aaacgatcag aatcgttcca aatcctttca cagcctccac caaagcacga
     9001 ataccgggtg cagttattcc gtcatcattc gttacaagaa ttacgggttt ctttttcttc
     9061 attcaacaaa aataatcaaa ctaaaggcta aaaagatgct tgtgctaacc gtttacacag
     9121 aattaaggta gtctatccca tgcaattact gtttaagggc tttttttgta tcttcaacac
     9181 ttcaaaacca taggtgatgt cagtgaatta caaatcggat gttgaggcgg ttaatgcctt
     9241 tgtagagaaa tacaagatcc ttcataaaga gatcggaaaa gtaatcgttg gacaggaaca
     9301 ggttgttgat gaagtgctca tttccatctt cagtaaagga cattgcctct tggtgggtgt
     9361 tccgggtctt gcgaaaacct tgttggtaaa taccattgct gatgcgcttg gattaagctt
     9421 taacaggatt cagttcacac ccgatttaat gccaagtgat attatcggat cggagatact
     9481 tgatgaaaca cgtaatttca aattcataaa aggtccactg tttgcgaaca tcattcttgc
     9541 cgacgagatc aaccgtacac ctccaaaaac gcaatcggca cttttagaag cgatgcagga
     9601 aaaagctatt actgcttcag ggaaacggta taaacttgat gaaccctttt tcgtactcgc
     9661 tactcaaaac ccaattgaac aggaaggaac ctacccccta cccgaagcac agctcgaccg
     9721 ttttatgttc aatatttggg tagactatcc aaaatttgaa gaagagctgg ccattgttcg
     9781 aaatacaact tccgatatga aagtaaaact tgaaaaagta ctttcttcag aagagattct
     9841 ctatttccag gatttgatcc gacgcattcc tgttgctgat aatgttctgg aatatgcagt
     9901 taaactaagc agcaaaacgc gtccgaacac acccggcgct accgatatcg ttaataaata
     9961 ccttagctgg ggtgcaggtc caagagcttc gcaatacctt gtggttggtg caaaatgcca
    10021 tgctgcagtg aaaggaaaat attcgcccga tattgaagat gtccaggctg tagcaatgcc
    10081 aattttacgt caccgtattg ttcgcaatta catggctgaa gctgaaggat attctattga
    10141 gaaaatcatt aagtctttat tataacaacg tattgtgttt ttcattttgc acaggacaaa
    10201 acaaaaattt cttccataaa aaaagccccg tttggggctt tttcatttca atggtagttc
    10261 catttaagcg gtctgttcca atgctcctgt aaactcaggt gaaatagcag cacgttcgat
    10321 gcgcagcttt cctcctcctt cggttgcaat aacaatggtg gtatccttga tctcttcaat
    10381 tttaccgtga att
//
LOCUS       DAOMYG010000041        10391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2137_length_10391_cov_10.573626, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000041 DAOMYG010000000
VERSION     DAOMYG010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10391
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2137_length_10391_cov_10.573626"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..417
                     /locus_tag="PK610_02325"
     CDS             4..417
                     /locus_tag="PK610_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015024376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRJ34788.1"
                     /translation="MSKTAEINSPDRLNRIVEGTEIIGDIRTDGNIRIDGKVKGTIHT
                     KGRLVIGATGVIEGEIVCENADIEGLFNGKIMVNQLLSLKATARLTGDIQTSKLAIEP
                     GANFTGACSMGGMVKDMKQPESSDRRNALASEKTA"
     gene            389..604
                     /locus_tag="PK610_02330"
     CDS             389..604
                     /locus_tag="PK610_02330"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRJ34789.1"
                     /translation="MPLPPKKRPDAAYSNYLTTSFKMAIAIGLGVYGGLKLDGLIDWK
                     FPLCTILLSLLGVALGIYILVRDTGNK"
     gene            610..993
                     /locus_tag="PK610_02335"
     CDS             610..993
                     /locus_tag="PK610_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34790.1"
                     /translation="MKNFLLVAVAFSIVAELSVYLIFNYVFPEFDRPINYWITPFLLL
                     VFGLAHWQLISSLRKNAKRFVTLFMGVTGGKMLLSLFIVLILAVLLKEAVKPVAVTFI
                     AIYLPFMVIEVIFILKELREVQKAS"
     gene            1082..2182
                     /gene="atpB"
                     /locus_tag="PK610_02340"
     CDS             1082..2182
                     /gene="atpB"
                     /locus_tag="PK610_02340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRJ34791.1"
                     /translation="MIRSIQGSLRFFLVIFTLLNASSKGFASGNNGEFNAGEFIIHHV
                     SDAHEIHFFGHVAMPLPIIIYDDGVKVFSSSNFYHSEPKKTFNPVKLKEEEYFQYENY
                     VLLHEKIYKLSEQELILDGHGHPQNASAVFDMSITKSVFGMFLIMIFLILLFRSAAKK
                     YAANEGKAPSGMQNALEPFILFVRDEIAKPSIGHHYERFVPFLLTVFFFIWASNMLGL
                     IPFLGGFNIMGTLSVTMVFAAGVFILTSINGNAHYWGHLLNPPGVPMGIKFILVPIEV
                     VGIFLKPLVLMIRLTANITAGHIIILSFVSLIFILGNNYGAGVGYGVGIGSTAFMVFM
                     NVLELLVAFLQAFVFTMLTALMLGSAVEEAHH"
     gene            2215..2454
                     /gene="atpE"
                     /locus_tag="PK610_02345"
     CDS             2215..2454
                     /gene="atpE"
                     /locus_tag="PK610_02345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012821237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRJ34792.1"
                     /translation="MSLLAVLLASPTAGVAAIGAGIAAIGAGLGIGKIGASALESIAR
                     QPEATGDIRANMIVAAALVEGVALFAVIVCLLAVL"
     gene            2548..3036
                     /gene="atpF"
                     /locus_tag="PK610_02350"
     CDS             2548..3036
                     /gene="atpF"
                     /locus_tag="PK610_02350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRJ34793.1"
                     /translation="MLTVSFGTIIWTSIAFILVLVIMKKLAWKPILETLAKREKDIQE
                     AIDSARLAREEMARLKVSNEELQKKAREERDLILKEARDTREAIVAEAKQKATLEADR
                     ILAAARENIKNEKMAAISDLKNQVATLSIEIAEKILRNQLSADDKQKALINNMVEEIN
                     LN"
     gene            3038..3595
                     /gene="atpH"
                     /locus_tag="PK610_02355"
     CDS             3038..3595
                     /gene="atpH"
                     /locus_tag="PK610_02355"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HRJ34794.1"
                     /translation="MKGQRVAYRYAKSLLSLATEQNMLEQAFADMQLIADTCNTSKDF
                     VVFLRSPVVKADKKISVINTVFTDKLSVISAGFIRIITTHRREIYLAEIANSFVEQYR
                     ELKKIATAEVTTASPLDEKLRNRIREIVRQTEGREVFMIEKVDPGIIGGIIVKVGDRR
                     YDGSIMRKLRQLRQDFSKNAYVSQL"
     gene            3614..5191
                     /gene="atpA"
                     /locus_tag="PK610_02360"
     CDS             3614..5191
                     /gene="atpA"
                     /locus_tag="PK610_02360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRJ34795.1"
                     /translation="MAEVKPAEVSAILREQLAGFKSAAELEEVGTVLQVGDGIARIYG
                     LSNVQSGELIEFEAGLRGIVLNLEEDNVGAVLLGASTDIKEGDTVRRTGLIASLKVGD
                     GMVGRVVNTLGDPIDGKGPITGEMFEMPIERKAPGVIYRQPVSEPLQTGIKAIDAMIP
                     IGRGQRELIIGDRQTGKTTVAIDTIINQKEFYDKGEPVYCIYVAIGQKGSTVAGIVKK
                     LEEAGAMAYTVVVVANASDPAPMQFYAPLAGAAVGEFFRDTGRPALIVYDDLSKQAVA
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKVIANDTIAAQMNDLPESLKGRVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLESNLFNSGVRPAINVGISVSRVG
                     GSAQIKSMKKVAGTLKLDQAQYRELEAFAKFGSDLDAATKSVLEKGKRNVEILKQREG
                     SPMSVEKQVAVIYLGTRGLMSNVPVNKIKAFEEEFVAFLDTKHRNILDDLRAGKFTDE
                     ITAILESVCKELSAKYA"
     gene            5211..6089
                     /gene="atpG"
                     /locus_tag="PK610_02365"
     CDS             5211..6089
                     /gene="atpG"
                     /locus_tag="PK610_02365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRJ34796.1"
                     /translation="MANLKEIRGRITSVNSTKQITSAMKMVSAAKLKRAQDAIIQMRP
                     YAEKLKEILGNLSASLDANEGVYSAPREAKNVLIIAITSNRGLCGAFNNNVIKRTLAL
                     IQEHKGSNISVLCIGKKAQEFFKNTEYNIKASDFPKHLNEVYDNLKFSTVAPIAENIM
                     RHYVDRSVDKVVLVYNKFKNAAMQELMAEQFLPIIPTASVEVSNSSVEYIFEPSKKEI
                     LEDLIPKSLKIQFYKAVMDSFASEHGARMTAMHKATDNATELVKELKLTYNKARQAAI
                     TNEILEIVAGAEALNS"
     gene            6155..7609
                     /locus_tag="PK610_02370"
     CDS             6155..7609
                     /locus_tag="PK610_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ34797.1"
                     /translation="MKNILSALFVLLLVAKSIAQQPEFLQTIEVCNSSVFGGLRPRIT
                     LVHDSIPVISFNRAGADPAVFVSRYSGGAFSAPLRISSPGTLPLSSTVNSAEIKSAGD
                     TVFLLYAEHTAEASISIRRSLDGGLSFPDSFCVASYPSNHVEFPELTLLSGGNPVVAY
                     IFSDLNMINTEIRAAYSTNGGAVFTSSTGITGLLPGQPCECCPIAVEKAGNSVFIGYR
                     NNISNEREFYLLQSNDGAQSFTGFVAIDDYEWIINTCPTNGIDLLPMGDSLHTAFASK
                     PGGTVQIFHRGVHHTGTNIGTVGLNENSPPAQNMSYSVLAGANDTLFLAWQDTRSGST
                     DVYFSFRTPGQSGFSQAFAANDPVAGVQRTIDMAYANGLLHIVYTDLTNQKVIYRKAR
                     LTNTTSAEEIPGPNFQLTYINQEQSIRISNDKERIKNVLIYTISGSVSQEIKGNNQQE
                     INISTKELIPGIYLAKLLTESGREVVLKFVLTQP"
     gene            complement(7610..9010)
                     /locus_tag="PK610_02375"
     CDS             complement(7610..9010)
                     /locus_tag="PK610_02375"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRJ34798.1"
                     /translation="MKKLAALLSLCFAFTMANAQYTSTNLVTGLKYPVSMQFAPDGRI
                     FLTQKGGDGFASNVDGQVRIFSAAGASLGVLWDFTDSVETYFERGVLGVALDPDFDNN
                     HFVYVFYNHKTPAPARIRVVRFTETNNAGSNPTIIFEYTDPFTAGNHTGGNLHFKPGD
                     NDHLYISIGDRATQPNSQLLTNPCGKILRINKNGSIPTDNPFYDDGNVATGNDDRIWA
                     YGLRNTFDFTFSTINDSIYGSENGLNTWDEVNQIVRGGNYGWPTCEGFYNQGSTSVAC
                     SMSGSILPIDTWGAPLPAVTGIIVYDHPLMPEFQGHMLVTDYDNGRITDIELTGTALN
                     VAGTRQNFPTIYTKLVDIAQGPEGCIYVIQGGYTTNGIIKKICPTNMNVESTAAALEF
                     AVYPNPATNTVRIELDDFSAGSAIEICDLSGRIVKRETSVNTQMQIDISMLSSGTYLI
                     NVNDGVRRGTRLLIVE"
     gene            complement(9058..9762)
                     /gene="recO"
                     /locus_tag="PK610_02380"
     CDS             complement(9058..9762)
                     /gene="recO"
                     /locus_tag="PK610_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRJ34799.1"
                     /translation="MLFKSEALLLHHFRYGENSQIVKVFTPVQGAVSLFFRPSKKHAR
                     AGLLQPLSLLDIVFWREHGKDMGTVKEIRLDHAYQNIGRDMYRGSVVLFLNEILVKIL
                     KEGTEDPALFSFVKEWLIHYDIAEFDADAHLYFLAHLASLAGFLPDGVYSPSSPVFDL
                     ESGNFIASRPSHPRFLEGDTASSFLGLFSVEHQHAFSAAERKVLLYAVLEYFRLHFPD
                     FGELKSLAVLEELFRP"
     gene            complement(9824..>10391)
                     /locus_tag="PK610_02385"
     CDS             complement(9824..>10391)
                     /locus_tag="PK610_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ34800.1"
                     /translation="AQQILIQQDGNYQYLLETEVVTAIAVDGANRKWVGTDGSGVYLL
                     SEDGAEQIYHFTTENSPLLSNIINDVEIDHSTGEVFFATSQGLISYRGSATIEDNPYS
                     IAFAFPNPVRPEYTGPIVIKGMDRDADVKITDIAGNIVFVTKSEGGQAVWDGNNLKGE
                     RVASGVYTVLCKNPNGPGRVVAKILFIK"
BASE COUNT         2930 a         2378 c         2241 g         2842 t
ORIGIN      
        1 gatatgagca agaccgcaga gattaattca cctgatcgtt taaacagaat tgttgaagga
       61 actgaaatca ttggtgacat cagaacggat ggcaacattc gtatcgacgg taaggtgaag
      121 ggcaccattc ataccaaagg acggctcgta attggcgcta ccggtgtaat tgagggtgaa
      181 attgtatgtg aaaacgccga tattgaaggt ctcttcaatg gaaaaataat ggtgaaccag
      241 ctgttgtccc ttaaggccac tgcacgacta accggcgata ttcaaacctc aaaacttgcc
      301 attgaacccg gcgctaactt taccggtgcc tgcagtatgg ggggaatggt aaaggacatg
      361 aaacaacctg aaagcagcga tcgtcgcaat gcccttgcct ccgaaaaaac ggcctgacgc
      421 cgcctattcc aattatttaa ccacctcctt taaaatggca attgccatcg gactcggggt
      481 ttatggtggt ttaaaactcg atggtctcat cgattggaaa tttcctcttt gtacaattct
      541 tctttctctg ctcggtgttg cgctcgggat ttatatcctg gttcgggata ccggcaataa
      601 ataatctgca tgaaaaattt ccttctggtt gctgtcgcct tctcaattgt tgcagaacta
      661 tcggtttacc tgatctttaa ttatgtgttt ccggaatttg accgtccgat taactattgg
      721 attaccccct ttctgctgct cgttttcgga ctggcgcact ggcagcttat ctcatcatta
      781 cggaaaaacg cgaaacgttt cgtaaccctg tttatggggg taaccggcgg aaaaatgctg
      841 ctttcgcttt ttattgttct gattctggct gttttactga aagaagctgt aaaaccggtt
      901 gcagttacat tcatcgccat ttatctgccc ttcatggtca ttgaagtcat attcatcctc
      961 aaggaattac gcgaggttca gaaagcttcc tgatagttat caaccccttt ctatttttcc
     1021 cctgcctttt ttcgtttcga attggtattt gtctactttt gcgctcgatt ttgaaaaggc
     1081 aatgatacgt tcaatccaag gttctctcag gttttttctg gtgattttca ccctgcttaa
     1141 tgcaagctca aaggggtttg ccagcggtaa taatggcgaa tttaacgccg gtgaattcat
     1201 tattcatcac gtttctgacg cgcatgaaat ccactttttt ggccatgttg ccatgccctt
     1261 accgattatc atttatgatg acggagtaaa ggtgttttca tccagcaatt tctaccacag
     1321 cgaacctaaa aagaccttta atcctgtaaa actgaaagag gaagagtatt tccagtacga
     1381 aaactacgtg cttcttcatg agaagatcta taagttaagc gagcaggagc ttattcttga
     1441 tggtcatggc catcctcaaa atgcttctgc ggtttttgac atgtccatca ccaaaagtgt
     1501 gtttgggatg ttcctgatta tgattttcct gattctgctg ttccgtagcg cagccaaaaa
     1561 atatgcagct aatgagggta aagcaccatc aggaatgcaa aacgcgctcg agccttttat
     1621 cctgtttgta cgtgatgaga ttgccaaacc gagtattggt caccattacg agcggtttgt
     1681 accgtttctg ctgaccgttt tcttcttcat ctgggcttcc aatatgctcg gccttattcc
     1741 ttttcttggt gggttcaaca tcatggggac actcagcgta acgatggttt ttgctgcggg
     1801 tgtatttatc ctaaccagta tcaacggaaa cgctcactac tggggtcacc ttttgaatcc
     1861 tcctggtgtt ccaatgggta ttaaattcat tcttgttccc atcgaagtag tgggaatatt
     1921 tctgaagccc ctggtgctta tgattcgtct taccgccaac attacggccg gtcacatcat
     1981 catcctgtct tttgtgagtt tgatctttat tctgggcaac aactacggtg ccggagtagg
     2041 atacggtgtg ggaatcggat caaccgcgtt tatggttttc atgaacgttt tggaactttt
     2101 agtagcattt cttcaagcct tcgtattcac catgcttact gcgctcatgc tcggttcggc
     2161 tgttgaggag gcgcatcatt aattcgtaat aattaaccct taatacctaa tcaaatgagc
     2221 ttattagctg ttcttctcgc tagcccaact gcaggtgttg ctgctattgg tgccggtatt
     2281 gctgccattg gtgcaggact tggtatcggt aaaatcggtg cttctgctct cgagtctatt
     2341 gctcgtcagc ctgaggctac cggagatatc cgtgccaaca tgatcgttgc tgctgcactt
     2401 gttgaaggtg tggcactgtt cgcagtgatc gtgtgtctgc tcgcagtact ctgattccgg
     2461 aatggaagaa ggtcccgacg gttggtcggg aaccctcttt ccggcgatta ttacaagatt
     2521 caatttaacc ccgaataagt aaacactatg ttgaccgtaa gtttcggaac catcatttgg
     2581 acctcaatcg ctttcattct ggtgcttgtc atcatgaaga agctggcatg gaaacccatt
     2641 ctggaaacgc tagccaagcg tgaaaaagac attcaggaag cgatcgattc tgcacgtctt
     2701 gcacgtgaag aaatggcccg cctcaaagta agcaatgagg aacttcagaa aaaagcccgt
     2761 gaagagcgcg atctcattct gaaagaagcc cgtgataccc gcgaagctat tgtagctgaa
     2821 gccaaacaaa aagcaaccct cgaagcagat cgtattcttg cagctgcacg tgagaacatc
     2881 aaaaacgaaa aaatggcggc tatttccgac ctgaaaaatc aggttgctac gctttccatt
     2941 gaaatcgctg aaaagattct gcgcaatcaa ctgtcggccg acgataaaca gaaggctttg
     3001 atcaacaaca tggtcgaaga gatcaatctg aactgatatg aaaggccaac gcgtcgcata
     3061 ccgatacgct aaatctctcc tgagtctggc aactgagcaa aacatgctcg aacaggcttt
     3121 tgcagatatg cagctaattg ccgatacctg caatacaagt aaagatttcg ttgtttttct
     3181 tcgcagtccg gtagttaaag cagataagaa gatttctgta atcaacaccg ttttcaccga
     3241 taagctcagt gtgatttctg caggatttat ccgcattatc acaacccatc gtcgtgaaat
     3301 ttatcttgcc gaaatagcaa acagctttgt tgaacaatac cgcgaactga aaaagatcgc
     3361 aactgctgaa gttaccacag caagccccct tgatgaaaaa cttcgtaacc gcattcgtga
     3421 gattgtacgt caaaccgaag gtcgcgaggt atttatgatt gaaaaagtag atccgggaat
     3481 cattggcgga atcattgtta aagtgggcga tcgtcgttac gacggttcca ttatgcgcaa
     3541 gctccgtcag ctccgtcagg atttttccaa aaatgcatac gtgtcacaac tctaataact
     3601 aacagaccga aaaatggctg aagtaaaacc cgcagaagtt tctgctatcc tccgcgagca
     3661 gctcgcagga ttcaaatccg ctgccgagct tgaagaagta ggtaccgtac tccaggtagg
     3721 tgatggtatt gcccgtatct acggactttc caacgtacaa tccggtgagc tcattgagtt
     3781 cgaagccggt ctccgtggaa ttgtacttaa ccttgaagaa gacaacgttg gtgctgtatt
     3841 gcttggcgcc tccaccgata ttaaagaagg cgataccgtt cgtcgcacag gcctcattgc
     3901 atcactaaaa gttggtgatg gaatggttgg acgtgttgtg aacacacttg gcgatcctat
     3961 cgacggtaag ggtccaatca caggcgaaat gtttgaaatg cctattgagc gtaaagctcc
     4021 gggtgttatc taccgtcagc cggtaagcga acctcttcag acaggtatca aagcaatcga
     4081 tgcgatgatt cctatcggac gtggtcagcg tgagttgatc attggtgacc gtcagacagg
     4141 taaaacaact gttgcgatcg acaccatcat caaccaaaaa gaattctacg ataaaggcga
     4201 gcctgtatat tgtatctacg tagcaattgg tcaaaaagga tcaaccgttg ccggtattgt
     4261 taagaagctt gaagaagctg gtgcaatggc ctataccgtt gttgttgtgg caaacgcttc
     4321 agaccctgct ccaatgcagt tctacgctcc tttagccggt gctgctgttg gtgaattctt
     4381 ccgtgataca ggtcgtcctg cattgatcgt ttatgatgat ctctccaaac aagctgtagc
     4441 ttaccgtgag gtttctcttc tgcttcgtcg tcctccgggc cgtgaggctt atccaggtga
     4501 cgtattctac cttcacagcc gtcttttaga gcgcgcagct aaggtaatcg ccaatgacac
     4561 catcgctgca caaatgaacg atcttcccga gtcactcaaa ggtcgtgtta agggtggagg
     4621 atcgcttact gcacttccca tcattgaaac acaggcaggt gacgtttcgg catatatccc
     4681 aactaacgta atttccatta ccgacggaca gatcttcctc gaatcaaacc ttttcaactc
     4741 cggtgtgcgt cctgcaatta acgtaggtat ttccgttagc cgtgttggag gatctgcaca
     4801 gatcaaatcg atgaagaagg ttgccggtac acttaagcta gaccaggcac agtaccgcga
     4861 gcttgaagca tttgcaaagt ttggttctga ccttgatgct gctacaaaat cagtacttga
     4921 aaaaggtaaa cgcaatgtag aaatcctcaa gcagcgcgaa ggttctccaa tgtcggtaga
     4981 aaagcaggtt gctgtaattt acctcggaac ccgtggattg atgtcgaacg ttccggttaa
     5041 caaaatcaag gcgtttgaag aagagtttgt agctttcctt gatacaaagc atcgcaatat
     5101 ccttgatgat cttcgtgcag gtaagtttac cgacgagatc acagcgatcc ttgagtcagt
     5161 ttgtaaagaa ttatcagcga aatacgcata aaacatccta agctaggacc atggccaatc
     5221 ttaaggaaat acgcggacgg attacttcgg taaactctac caagcagatc acctctgcaa
     5281 tgaaaatggt atcggcagcg aaactcaagc gtgcacagga tgcaattatc cagatgcgtc
     5341 cgtatgcaga aaaactcaaa gagattctcg gcaacttatc cgccagtctt gatgccaatg
     5401 aaggcgttta ctcagctcca cgagaagcaa aaaatgttct gatcattgcc attacctcta
     5461 accgcggttt gtgtggtgca tttaacaaca atgttattaa acgcacgctt gcacttattc
     5521 aggagcataa aggaagcaat atatctgtac tttgcattgg taaaaaagcc caggagtttt
     5581 ttaaaaacac cgaatacaat atcaaggcct ccgatttccc aaagcacttg aacgaggtat
     5641 acgataattt gaaattcagt actgttgctc ctattgcaga gaacatcatg cgtcactatg
     5701 ttgaccgcag cgttgacaaa gtggttttgg tgtacaacaa attcaaaaat gccgctatgc
     5761 aggagttaat ggctgagcag tttcttccca ttattcctac agcatctgtt gaggtttcta
     5821 attcgagtgt tgaatacata ttcgaaccct ccaaaaagga gattctcgaa gatcttattc
     5881 caaaatcact taaaattcag ttctacaaag cagtgatgga ctcatttgcc tcagaacacg
     5941 gtgcccgtat gaccgccatg cataaggcca cagacaacgc taccgaattg gtgaaagaac
     6001 tcaaattaac ctacaacaag gcgcgtcagg ccgccattac caacgaaatc cttgaaatcg
     6061 ttgcaggcgc cgaagcatta aactcttaat tgtacctaca acaatttcat aaatagcttt
     6121 ccttttggag agctattttt ttttatattg gtatatgaaa aacatattaa gcgcactatt
     6181 tgtcctgttg cttgtggcaa aaagcattgc tcaacaacct gagtttcttc aaaccattga
     6241 agtttgcaat tcaagtgttt tcgggggtct tcgtccgcgt attaccctgg tgcatgattc
     6301 aatcccagta atcagtttta accgggcggg agcagatcct gccgtttttg tttcccgtta
     6361 ttcagggggt gctttttcag ctccattaag aatttcgtct cccggaacgc tcccactctc
     6421 ttcgaccgtt aattctgccg aaattaaaag cgcaggcgac accgtatttc tgctttatgc
     6481 ggaacatact gcagaagcct ccatcagtat tcgtcgctca cttgatggcg gactctcttt
     6541 tcccgatagt ttttgtgttg cctcatatcc atccaatcat gtagaatttc ctgaattgac
     6601 cttgctttcg ggaggaaatc cggttgttgc ctatatcttt tcggatctca acatgataaa
     6661 taccgaaata agagccgctt actcaaccaa cggaggagct gtttttacat cgtctacagg
     6721 cattaccgga cttcttccgg gccaaccctg cgaatgctgc ccaatcgccg tggaaaaggc
     6781 cggaaacagt gtatttatcg gttacaggaa caatatcagc aatgagcggg agttctacct
     6841 gctgcaatcg aatgacgggg cacaaagttt tacgggcttt gtggcaatag atgactacga
     6901 atggatcatc aacacctgtc cgaccaacgg cattgatctg ttgccaatgg gcgattcgtt
     6961 acacacagca tttgcaagca aaccaggggg taccgtacag atttttcaca gaggggtcca
     7021 tcacaccgga acaaacattg gaactgtagg tctgaatgaa aacagtccac ccgcacaaaa
     7081 catgagttat tctgttttag ctggggcaaa cgacaccttg ttcttagcct ggcaggatac
     7141 ccgcagcggt agcacagatg tttacttcag cttcaggaca cccgggcaat ccggattttc
     7201 gcaggcattt gcagccaacg atcctgtggc aggagttcaa cgtacaatcg atatggctta
     7261 tgcaaatgga ttattgcata tcgtttacac cgatcttaca aaccagaaag tgatctatag
     7321 aaaggcccgg ctgaccaata caacttccgc tgaggaaatc cctggcccga attttcagct
     7381 gacctacatc aaccaagaac aatccattcg gatttcgaac gacaaagaac gcattaagaa
     7441 tgttttaata tacaccatta gcggatcggt atcgcaggaa ataaagggaa ataatcaaca
     7501 ggaaataaac atctcaacta aagaattaat acctggaatt tacctggcaa aattgctgac
     7561 agaatcagga cgtgaggtag tactaaagtt tgttcttacg caaccttaat tactcaacaa
     7621 tcagtaagcg tgtccctctt cgaacgccat cgtttacatt gatcaaatac gtgcctgaag
     7681 aaagcatgct tatatcaatt tgcatttggg tatttacaga tgtttcacgt ttcacaattc
     7741 gtccacttag atcgcaaatc tcaattgcgc tgcctgcaga aaaatcatcc agttcaatcc
     7801 gaacagtatt ggtagcagga ttaggataaa ccgcaaattc aagcgcggca gcagttgact
     7861 caacattcat gtttgtggga caaatctttt taatgattcc attagtggta taaccacctt
     7921 gaatcacata aatacaacct tccggtcctt gagcaatgtc aacaagcttg gtatatattg
     7981 ttgggaaatt ttgacgcgta cctgctacat taagtgctgt tccggtgagt tcaatgtctg
     8041 taattcgacc attatcgtag tcggttacca gcatgtgacc ctgaaactcg ggcataagcg
     8101 gatggtcata aacaataata cctgtaacag ccggaagtgg ggcaccccag gtgtctattg
     8161 gcaaaataga accggacata gaacatgcaa cagatgttga cccttggtta taaaaccctt
     8221 cgcaggtcgg ccaaccgtaa ttcccaccac gaacaatctg gttcacttca tcccaggtgt
     8281 tcaatccgtt ttcagatcca taaattgaat cgtttattgt agaaaaagta aaatcaaagg
     8341 tatttctcaa tccatatgcc caaatacgat catcgtttcc tgtggctaca tttccgtcat
     8401 cgtaaaatgg attatccgta ggaatacttc cgtttttgtt gatgcgtaaa atctttccgc
     8461 agggattggt caacaactga gaattgggtt gtgttgcgcg gtctccaata gaaatataca
     8521 aatgatcatt atctccgggc ttaaaatgca ggttgcctcc agtatgatta ccggcggtga
     8581 aaggatctgt atattcaaaa attattgttg gattggaacc tgcattattc gtttccgtaa
     8641 aacgcaccac acgaattctt gccggagcag gggttttatg gttgtaaaaa acgtatacga
     8701 aatggttatt gtcaaaatcc ggatcaagtg caacgcctag tactccacgc tcaaaataag
     8761 tctcaacaga atcagtaaaa tcccataaaa cccccaaaga agcgccagcc gcactgaaaa
     8821 ttctaacctg gccatcaacg tttgaagcaa atccatctcc ccccttttga gtgagaaata
     8881 ttcgtccgtc cggagcaaat tgcatggaca cagggtattt caatccggta accaaattgg
     8941 tactggtata ctgggcattt gccattgtaa aagcaaaaca cagcgaaaga agggcagcaa
     9001 gttttttcat gccaccaaaa tagctaaaaa cattgtctaa acgtggaaca acttattcta
     9061 aggtcggaat aactcctcca aaacagccag ggactttaac tccccaaaat ccggaaaatg
     9121 cagtcgaaaa tactcaagga ctgcatacaa caacactttt ctctcagcag cagaaaatgc
     9181 atgctggtgc tctactgaaa aaaggcctaa gaatgatgat gcggtatccc cttccaaaaa
     9241 ccgagggtgt gaaggtcggg aggcaataaa attaccggat tcgaggtcga aaacagggct
     9301 tgaagggcta tatacgccat ccggtaaaaa tccggcaaga gaggctaaat gcgccagaaa
     9361 atacaagtgc gcatcggcat caaattctgc gatgtcataa tgaatcaacc actcctttac
     9421 aaaggaaaac aaagcgggat cttcggttcc ttcttttaaa atttttacga gaatttcatt
     9481 gaggaacaat actacagaac cccggtacat atcccggccg atgttctggt aggcatgatc
     9541 aagacgaatc tccttgacgg ttcccatatc cttaccatgc tctctccaga agacaatatc
     9601 aagaagcgat aagggctgaa gcaaccccgc ccttgcgtgt ttttttgacg gtcggaaaaa
     9661 cagcgaaaca gcaccttgta caggcgtaaa aacctttacg atctggctat tttcaccata
     9721 cctgaaatgg tgcaataaaa gggcttcgga cttgaaaagc atagcgtcca aaaatactcc
     9781 tgaataagca tcagaaaaga gctttcccga aaataggggc taattatttg ataaacagaa
     9841 tcttagcgac aaccctgccc ggtccgtttg gatttttaca caaaacggta tatacacctg
     9901 aggcaacacg ctcccctttt agattgtttc catcccatac agcctggcca ccttcgcttt
     9961 tcgtgacaaa aacaatattg ccagctatat ctgtgatctt aacatctgca tcacggtcca
    10021 ttcctttgat cacgatggga cctgtgtatt ccggacgaac aggattggga aaagcaaatg
    10081 ctatgctgta aggattatcc tcaatggttg ctgagccacg gtaagagatc agtccctgtg
    10141 acgttgcaaa aaagacttct cctgttgagt gatcaatctc cacatcattg atgatgttcg
    10201 acaacaaagg gctgttttcg gttgtaaaat gatatatctg ttctgcacca tcttcagaca
    10261 gcaagtaaac accggaacca tcggtaccca cccatttgcg attggctcca tctacagcaa
    10321 ttgcagtaac tacctctgtt tcgagtaaat actgataatt tccatcctgc tgaatcagga
    10381 tttgctgggc a
//
LOCUS       DAOMYG010000042        10389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2139_length_10389_cov_11.820012, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000042 DAOMYG010000000
VERSION     DAOMYG010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10389
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2139_length_10389_cov_11.820012"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PK610_02390"
     CDS             complement(<1..250)
                     /locus_tag="PK610_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34801.1"
                     /translation="MKKDIEIPRMQDVGVAVVSEVNEEGLNEWNVYLINLKKEQLKGV
                     LVSSRGYGEINNDIRKTSELRHFLDEVNPKSFMKIEPII"
     gene            complement(247..1434)
                     /locus_tag="PK610_02395"
     CDS             complement(247..1434)
                     /locus_tag="PK610_02395"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRJ34802.1"
                     /translation="MRTALRFFGKFFKWTLITLGVVLILANAFILISGRTYIYKAVAC
                     TYLVGQSGPGILDLEYFPKREVANQIAEPWPVHSGYANARLLPEQQKILDQYKSTSFL
                     IFRRDTLMLEYYTGGFDEHTVSNSFSMAKSLVSLLCGIAIDEGKIKGVDQPVSDFIPS
                     FAEGEKSKLTLWHLLTMSAATDWSESGGNPLSHNAQAYYGWDLEGMIEDTQIEGEPGK
                     KFYYQSGITLILGYCVYKATGVPLAEYASEKVWKNIGAEQPAYWSLDKEGGLEKSYCC
                     LYATGRDFARLGKLYMHEGTWNEKRIVSSEWVKQSITPAELIDEDGSKNQRYGYQWWI
                     TQYEGKKVFYMRGILGQYVICYPEEDLIIVRTGHKRGRKQNDTPVEIDDYIRIAKEVT
                     KAL"
     gene            complement(1431..2153)
                     /gene="lipB"
                     /locus_tag="PK610_02400"
     CDS             complement(1431..2153)
                     /gene="lipB"
                     /locus_tag="PK610_02400"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRJ34803.1"
                     /translation="MRKVICEDLGLIDYKEAWDYQEKKFNELVERKLKNRELSDSSLH
                     DKVIHYLLFCEHPHVYTLGKSGSEDHLLLNESQREQHQVSYYKINRGGDITYHGPGQL
                     VMYPIFDLDDFFTDIHKYLRFLEEAVILTLKEYGVHSGRYPGYTGVWLDPQDDSKARK
                     ICAMGVKCSRWVTMHGIGFNINTDLSYFNHIVPCGIEDKQVTSLHKELGREVDMHELK
                     AKLLVNMANIFDFEFVTGEKTV"
     gene            complement(2155..3138)
                     /locus_tag="PK610_02405"
     CDS             complement(2155..3138)
                     /locus_tag="PK610_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HRJ34804.1"
                     /translation="MKKLVAEFPAQLRAAVNISKASVLKGFNGDLQNVLITGLGGSGI
                     GGTIASELAAKQAKVPVVVSKGYFIPGWVGKHTLVIVSSFSGNTEETINAMQLAMEAG
                     ATIVAVTSGGKVAEIAREKNFDLILLPTGDSPRACLGYSLVQQMHILQSFGVIAESVL
                     NDFLTAADLIEKEQQAILEEATKLTTFFKGRVPVIYTTTYREGIAIRLRQQLNENAKI
                     LCWHHVVPEMNHNELVGWTTKNEELAVLLLRDKDDYERNQKRFEINKGVIGKYTPHIA
                     ESWVGGKNKVEKAIWHIHLGDWISVLLADQRGVDPVEVRVIDHLKGELSKF"
     gene            complement(3217..6522)
                     /locus_tag="PK610_02410"
     CDS             complement(3217..6522)
                     /locus_tag="PK610_02410"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ34805.1"
                     /translation="MRTKHLRLFFLALTLAVFSNAIAQVSLGGTPQSFSKSLTLQALP
                     PTHVGFPDMQQVALEDAIDAKEGTLYKIARIMPVTLNPQNSGTWEVLPDGSRLWRLKI
                     QSDGALALFLTYNHFVLPEGSTLFLYNEDKSHIAGAYNSTINPPASNPEFSTRIIDSD
                     VTVLEYHAPMGVSADPIIDIAGVGHIYRGYNPRSVQVQNQQRIGESDPCQVNVNCTPE
                     GNSWQDEKRGVVRILVVGPSGAGWCSGSLINNTAQDCKRYVLTADHCAGGGGSNPQYS
                     TTANLNQWQFYFNYEAPTCTTPGTAGTLDDYVLTGCVKRAQGGNGGDNGSDMYLIEIT
                     PTIPAGWNVYYNGWSRSATAATSGVSIHHPSGDVKKISTFSSTLVNSGWNGSGLSSHW
                     RVTWVATTNGHGVTEGGSSGSPIFNNSGLIVGQLTGGASYCSATGQPDYYGKMSYNWS
                     SNTVPATNNTLQPWLDPGNTGVTTLSGVYAPCSPQAPVADFSANNTAPCIGSTVTFTD
                     QSSGQPTSWAWTFSPATVTYVGATSSTSQNPQVQFNAAGNYTVTLVATNAQGNDSEVK
                     NAYITVTNGAALPYAQNFEGATFPPAGITVQNPDNGTVAWGTDGAKGFVRRAAAGNTG
                     SASGCAGINYFNYNIAGGPLDALVIQPLSLVGATNPTMTFKRSYRYYNNPTYYDELRV
                     YVSTDCGATYGAAVYTKTGTQLATSGTLNTTFTPAAAGDWDIDTINLTSYIGQTVVIK
                     IEGTSRYGNNLYIDDINISNQAAVASVSISASANPICAGQSVTFTATPVNGGSAPTYQ
                     WRKNGNVVGTNSPTYTDNALTNGNTITCTMTSNLSGVTGSPATSNTITMTVNSSVIPS
                     VSISANPGTTICSGQTITFTGTPSNGGTTPSYQWRRNGNNTGTNSPTFATSALNNGDV
                     ISVIMTSNSPCASPTTATSNSLTITVNATPATPTITQNGTTLTSSSATGNQWYLNGSP
                     ISGATGQTHVATQVGNYTVIVTQTGCSSAASAPVNVTSLSIEDLILQNSFFVYPNPND
                     GQFTLQFDVAVTDDFVIEVKNPIGQIVYSETLGSFSGKYKRNLDLQGLGKGVYFITLR
                     NSGSECVKRVVIL"
     gene            6687..8198
                     /gene="lysS"
                     /locus_tag="PK610_02415"
     CDS             6687..8198
                     /gene="lysS"
                     /locus_tag="PK610_02415"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRJ34806.1"
                     /translation="MIQDLTEQEVIRREKLDEIRKLGIDPYPAEEYPVNAYAIDLKEQ
                     YEAEKPGFENIVLAGRLMSVRDMGKACFAELKDNSGRIQIYVRRDDICPGEDKTLYDQ
                     LFKKLLDIGDFIGVKGFMFKTKMGETTLHVKELTLLCKTLRPLPIVKTDEEGNVFDAF
                     TDPEKRHRMRYVDLIVNEGVRETFIKRTKLMNSLRNTFNASGYIEVETPVLQPIPGGA
                     AARPFTTHHNALDIPLFLRIANELYLKRLIVGGFEGVYEFSKNFRNEGIDRTHNPEFT
                     ALEIYVAYKDYNWMMDYTEEMIEKVALDVNGTTEVQVGEKSISFKRPYKRITMFDAIK
                     EHTGFDVSEMNEDQLRDVCKKLHIETEASMGKGKLIDEIFGNKCEHNYIQPTFIIDYP
                     VEMSPLTKKHRSKEGLVERFELYVNGKEIANSYSELNDPIDQRDRFMEQMALGERGDD
                     EAMVIDHDFLRALEFGMPPTAGLGIGIDRLTMMMTNQASIQEVLLFPQMRPEK"
     gene            8298..>10389
                     /locus_tag="PK610_02420"
     CDS             8298..>10389
                     /locus_tag="PK610_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34807.1"
                     /translation="MKNLIFSAFAVAVSQFTFAQTLSWSAYTDSTVTYSSPRAVDLTG
                     DGVLDIIMGAGKEAAVTNYGILAFNGATGASLWTVQTRNEIFTSAQFRDITGDNIPDI
                     FLGGRDAQLYAINGSTGAIIWEYWPFANGNPNLQGLYNFYTPQIIPDMNGDNIPDLLL
                     ANGGDHAAPVWQTNRPPGHLMIISASDGALISMVEVPDGAETYCSPVVANLKGDGVLY
                     VVFGTGGETLPGSLWVAPLLDVFNEDLSSAVALATHPTLGFVAPPTVARFSSAAHMDI
                     VAHGYDGTLYRFKGSDYSLVWSKSFPGTETSASPVIGNFTGNLTPDVFGVLYKGVAPS
                     YSDFYQVMLDGETGTLVYKDSLLSMHFAAPVAFDSNGDGRDEVLISGSNHVGHFRHQL
                     MLIDFQNNTSANYWNSEAGSNIGSTPWVGDLDNDDMIDLVFAFRKDSLNPMGWKGTFV
                     KRLNTGMNMPIKDIAWGSYMGSEYDGHYNYTFTNCGLGSILSNVFVSNPSCNGLSDGS
                     INPVPGNGTAPFTFLWSDGSVGDKLLNVPAGQYSLIVTDATGCSELRMFTLSNPYTIS
                     FGGINPVTCPGGTNGTATVSSTGCYCMFSGCNFLWEQGTTGYTISGVPTGMYTVTITH
                     LDGCVVVDSVYIPEGLPVLSTTLVTQIDCFGADNGTIELVHNFNGTNYVWSDGFNGGV
                     HTGLEPGNYTYTATD"
BASE COUNT         2902 a         2277 c         2243 g         2967 t
ORIGIN      
        1 cgatgatggg ttcaatcttc atgaaacttt ttggatttac ctcatctaaa aaatggcgga
       61 gctcagaagt tttacgaata tcattattga tttcaccata ccctctggat gaaaccaaca
      121 ctccctttag ctgttccttt ttcagattaa tgaggtaaac attccattca tttagtcctt
      181 cttcattcac ctcactcaca actgcaactc ccacatcctg catgcgggga atttcgatgt
      241 ctttcttcat aatgcttttg tcacttcttt tgcgattcga atataatcgt ctatctctac
      301 cggtgtatcg ttttgctttc ttccgcgttt atgaccggtt cttacaatga tcaaatcttc
      361 ttcagggtag cagatcacat actgaccaag aatccctctc atgtaaaaaa ctttcttgcc
      421 ctcgtactgc gtaatccacc attggtatcc gtaacgttga tttttgctgc cgtcttcgtc
      481 aattaattct gccggggtaa ttgactgctt cacccattcc gatgaaacga ttcttttttc
      541 attccatgtt ccctcatgca tataaagctt ccctaaacgc gcaaaatcgc gtcctgttgc
      601 atacaaacaa caatatgatt tttccaaccc gccttcttta tccagactcc aataagcagg
      661 ctgttcggct ccaatatttt tccatacttt ttcagatgca tattctgcta agggcactcc
      721 ggtagctttg tacacgcaat aacccaaaat caatgtgatt ccactttgat aataaaactt
      781 tttacccggt tcaccttcaa tttgggtgtc ttcaatcatt ccttccagat cccatccgta
      841 ataagcttgc gcattatgcg atagtggatt tcctccgctt tcactccagt cagttgctgc
      901 gctcatggta agcaagtgcc ataaggtcag cttgcttttt tctccttcgg caaacgaggg
      961 aatgaaatcg cttaccggtt gatcaacacc tttaattttt ccttcgtcga ttgcaattcc
     1021 acaaagcaat gacaccagcg atttagccat ggaaaaggaa tttgaaactg tatgttcgtc
     1081 aaacccgccg gtgtaatatt cgagcatcaa tgtgtctctt ctgaagatca gaaatgaagt
     1141 gcttttatac tgatcaagaa ttttctgttg ctccggaagc agccttgcat ttgcatatcc
     1201 ggaatgaacc ggccatggtt ctgcaatttg gttggcaact tcgcgcttgg ggaaatattc
     1261 caaatcaagg attcccggtc cgctttgccc aaccaaatag gtacaagcca cagctttata
     1321 aatataggtg cgtcctgaaa ttaaaataaa ggcattggcc aaaatcagta caacgcccag
     1381 cgtgatcagt gtccacttga aaaattttcc gaaaaatcgt aaggctgttc tcataccgtt
     1441 ttttctcctg ttacgaattc aaaatcgaaa atgtttgcca tgttgacaag gagtttcgcc
     1501 ttcaattcgt gcatatctac ctcgcgaccc aattccttat gaagtgaagt cacttgctta
     1561 tcctctattc cgcagggaac aatatggttg aaataagaaa gatctgtatt gatattaaaa
     1621 ccaattccgt gcatggttac ccaacggctg cacttcactc ccatcgcgca aattttgcgt
     1681 gccttgctat cgtcctgtgg atcgagccat acaccggtat atccgggata gcgaccggaa
     1741 tgaactccgt attctttcaa agtgagaata acagcctctt ccaggaaacg caggtatttg
     1801 tggatgtcgg tgaaaaaatc atccagatca aaaatcggat acatcaccaa ttgaccggga
     1861 ccatggtaag taatatcacc tccacgattt attttgtaat aacttacctg atgctgctcc
     1921 cgttgcgatt catttagcag gagatggtct tctgatccgc ttttccctaa ggtataaaca
     1981 tgcggatgct cgcaaaacaa taggtaatga atcactttat catgaagaga actatcggac
     2041 aattcccgat tcttcaactt tcgttcgaca agctcgttga attttttttc ctgataatcc
     2101 caggcttctt tgtagtcgat taaaccgaga tcctcacaaa ttacttttcg catttcagaa
     2161 tttgctgagt tcacccttga gatgatcaat cacacgcacc tcaacaggat ccacgccacg
     2221 ttggtcagca agtaaaacac tgatccaatc gccaaggtga atatgccata tcgctttctc
     2281 taccttgttt tttccgccta cccaactttc ggcaatatga ggagtgtatt ttcctattac
     2341 gcctttgtta atctcaaacc gtttctgatt acgttcataa tcatctttat ctctaagcag
     2401 caatactgca agctcctcgt ttttggtggt ccagccaacc agctcattgt ggttcatctc
     2461 aggaacaaca tgatgccagc aaagtatttt tgcattttca ttgagttgct gacgaagacg
     2521 gatagcgatt ccttcgcggt aggtggtagt ataaattaca ggaacccttc ccttgaaaaa
     2581 tgttgtaagc ttagtcgcct cttccaatat tgcttgctgt tctttttcta tcagatctgc
     2641 agcagtcaaa aaatcattca atacactttc tgcaattact ccgaaacttt gcaaaatatg
     2701 catttgctga accagtgaat atcccaaaca tgcgcgtggc gaatctccgg ttggaagtaa
     2761 aatcagatcg aaatttttct cacgggcaat ttcagcgacc ttaccaccgg aagttactgc
     2821 aacaatggtg gctcctgctt ccattgcgag ttgcatagca ttaatggttt cttcagtatt
     2881 gccggaaaag gaagaaacaa taaccaatgt atgcttgcct acccaaccgg gaataaagta
     2941 tcccttactc accacaaccg gaactttagc ctgtttggca gccaattccg aagcaatggt
     3001 tccaccaata cctgagcccc ccagaccggt aataagcaca ttctgcaaat ccccattaaa
     3061 ccctttaagc actgaggctt tggagatgtt tacagctgca cgcaattgtg ccggaaattc
     3121 agcaactaac tttttcatcc gcaaaaattg aaccccaaat gtaaataaaa agcccttacc
     3181 gctctttcgg gcgataaggg ctgattattg tttgaattac agaataacga cccgcttaac
     3241 gcattcgctt cctgaatttc gcagcgtaat aaaatacacg cctttcccaa gtccttgtaa
     3301 atcaaggttg cgcttatact tcccggagaa ggagcctaag gtctctgaat acacaatttg
     3361 tccaatcgga ttctttactt caataacaaa atcatctgta actgctacat caaactgcaa
     3421 tgtaaactgc ccatcattgg gattgggata tacaaagaat gaattctgaa ggataagatc
     3481 ttcaatagac agactagtta cattaaccgg tgcagatgct gctgatgaac atcctgtttg
     3541 cgttacgatt actgtgtagt ttccaacctg agttgcaaca tgcgtctgac ccgttgcccc
     3601 tgaaatggga gagccattca gataccactg gttcccggta gcagaactgc tggttaaggt
     3661 agttccgttt tgagtgatag tgggtgttgc aggtgttgca ttcactgtaa tggtcaggct
     3721 gtttgatgta gccgttgtag gacttgcaca gggtgaattt gatgtcatga tcaccgaaat
     3781 cacatcgccg ttgttaagcg cagaagttgc aaatgtagga ctgtttgttc ctgtgttatt
     3841 tccatttctg cgccattggt acgacggagt tgttccacca ttgcttggtg ttccggtaaa
     3901 ggtgatggtt tgtccggaac agattgttgt tccaggatta gcagaaattg aaacggaagg
     3961 tataacagaa ctgttcaccg tcatggtaat tgtatttgat gttgcaggac tacctgttac
     4021 cccggataag ttggaggtca tggtacaggt aatcgtgtta ccattggtca atgcattatc
     4081 agtataggta ggactgtttg ttccaaccac attaccgttt tttctccact gatacgttgg
     4141 ggcagatccg ccattgacag gagttgcagt aaaggttacg ctttgtcctg cacaaatcgg
     4201 gtttgcactt gctgaaatgg aaacagaagc aactgctgcc tgattactga tgttaatatc
     4261 atcgatatac aaattgtttc catagcggct tgtaccttca attttgataa caactgtttg
     4321 tccgatatag gaggtcagat taatcgtatc gatatcccaa tctcctgcag cggccggtgt
     4381 aaatgttgta ttcaacgttc cgcttgttgc caattgggtt ccagttttgg tataaactgc
     4441 agcaccataa gttgcgccac aatcagtaga cacatacaca cgcaactcat cgtaataagt
     4501 cggattgttg taataacgat aagagcgttt gaatgtcata gttgggtttg ttgctccaac
     4561 cagtgacagc ggttgaatca ccaaagcatc cagtggtcca cctgcaatgt tataattgaa
     4621 ataatttatt cctgcacatc cactggcaga accggtattt ccggcagcag cacgacgaac
     4681 aaatcctttt gctccatcgg tgccccatgc aacagttccg ttatccgggt tttgaactgt
     4741 tattcctgcc ggagggaacg tagctccttc aaaattctga gcatatggaa gggctgctcc
     4801 attggtaacc gtgatgtatg catttttgac ctcgctgtcg tttccttgtg cgttggttgc
     4861 aacaagtgta acggtgtaat ttcctgctgc attaaattgc acttgcgggt tctgcgaagt
     4921 tgaactcgta gcaccaacat aggtaacggt tgcaggactg aatgtccatg cccaactggt
     4981 gggttgccct gaagattgat cggtaaatgt taccgttgat ccgatacaag gagcagtatt
     5041 gtttgcggaa aaatctgcca ccggagcttg cggactacaa ggagcataaa ctcctgacaa
     5101 agtagtaaca cctgtatttc ctggatcgag ccagggctga agcgtgttat tggttgccgg
     5161 tacagtattg ctggaccagt tatacgacat cttgccatag taatccggtt gtccggttgc
     5221 tgaacaataa gaggcaccgc cggtaagctg gccaacgatt aatcctgaat tattgaaaat
     5281 aggagatcct gaagatccgc cctcagtaac accatgacca ttggtagtag caacccaggt
     5341 aacacgccag tgcgaactta atccgcttcc gttccatccg ctgtttacaa gcgtagaaga
     5401 aaaagtagag atctttttaa catcgccgga aggatggtga atgctcacac cacttgtagc
     5461 agcagtagca gaacgactcc aaccattgta gtacacattc catccggcag gaatggtagg
     5521 tgtaatctca ataaggtaca tgtcggaacc attatcgccg ccatttcctc cctgagctct
     5581 ttttacgcag ccggtaagca cataatcatc aagtgttcca gcagtacctg gagttgtgca
     5641 ggtaggcgct tcataattaa aataaaactg ccactgatta aggttggctg tagttgaata
     5701 ctgaggatta gaaccacctc caccggcaca atggtcggca gtgagaacat agcgcttgca
     5761 atcctgtgcg gtattgttga tcaaggaacc ggagcaccat ccggcgcctg aaggacctac
     5821 aaccagaata cgaaccacac cacgtttttc atcctgccag ctgttgcctt cgggtgtaca
     5881 gtttacattc acctggcaag gatctgactc accaattcgc tgttgatttt gtacctgaac
     5941 agaacgaggg ttataaccac ggtaaatgtg tcccacccct gcaatatcaa ttattggatc
     6001 agcagaaact cccattggtg catgatactc aagaaccgta acatcactgt cgataatgcg
     6061 tgtagaaaac tcagggttgg atgcaggcgg gttgattgtg ctgttatagg caccggcaat
     6121 gtgagattta tcttcattat agaggaacaa ggtcgaacct tccggtaaaa cgaagtgatt
     6181 gtacgttaga aaaagcgcca aagcaccatc cgattgaatc ttcaacctcc acagacgcga
     6241 tccgtcgggt aaaacttccc aggttccgct gttttgaggg ttgagcgtta ccggcatgat
     6301 acgggcgatc ttgtaaagcg tgccttcttt tgcatcaata gcatcttcca acgcaacctg
     6361 ctgcatatcc ggaaacccta catgagtagg aggcagggct tgtaatgtaa gggattttga
     6421 aaagctttga ggtgttcctc caagcgaaac ctgcgcgatt gcattggaga aaacagcaag
     6481 cgttaaggca agaaaaaata aacgtaaatg ttttgttcgc atacaagtag gtttttagtg
     6541 gcctgaattt aacattcgaa aaacaggaat gaaacaccgt tggataagta attcacctaa
     6601 actaaacgaa atcgcattta acaggttgaa atccagcaaa acaagaattc atcaacattg
     6661 atatctttgc aaaaattatt tatcccatga ttcaagacct taccgaacag gaagttattc
     6721 gcagagagaa actggatgaa attcgcaagc tgggtattga cccctatcct gcagaggaat
     6781 atccggtgaa tgcttatgca atcgatctta aagaacaata cgaggcagaa aaacccggtt
     6841 tcgaaaacat agttcttgca ggaagattaa tgagtgttcg cgacatgggt aaggcgtgtt
     6901 ttgcagaatt gaaagacaat agtggaagaa ttcagatcta cgtgagacgc gatgatattt
     6961 gtccgggaga agataaaaca ttgtatgatc agctattcaa aaaactgctt gatattggcg
     7021 attttatcgg tgtaaaagga ttcatgttca aaaccaaaat gggtgaaacc actttgcatg
     7081 ttaaagaact cacactgcta tgcaaaacat tgcgaccact tccaattgtg aagaccgatg
     7141 aagaaggcaa tgtgtttgat gcgtttacag atcccgaaaa gcgtcaccgc atgcgttatg
     7201 tcgacctcat cgtaaatgaa ggtgtaagag aaaccttcat caagcgcaca aagctgatga
     7261 attcattgcg aaacacattt aatgccagtg gttatattga agtggaaaca ccggtattgc
     7321 aacccattcc gggaggagct gctgcacgtc cgtttacaac acaccataat gcgctagata
     7381 ttcctttgtt ccttcgaatt gcaaatgagt tgtatctgaa gcggcttatt gttggtggtt
     7441 ttgaaggtgt atacgaattc tcgaaaaatt tccgaaacga gggaattgac cgtacacata
     7501 atccggaatt tactgcgctt gaaatttatg tggcgtataa ggattacaac tggatgatgg
     7561 attacaccga agaaatgatt gaaaaagtgg cgcttgatgt aaacggcacc accgaagttc
     7621 aggtaggtga aaaatcgatt tcgtttaagc gtccttataa gcgcattacc atgtttgatg
     7681 cgataaagga acataccgga ttcgatgtta gcgaaatgaa tgaagatcag ctgcgagatg
     7741 tatgcaaaaa actgcatata gaaacagaag cgagcatggg aaaaggcaaa ttgattgatg
     7801 aaattttcgg taataaatgc gaacacaatt acatccagcc aacattcatc atcgattatc
     7861 cagttgaaat gtcgccgctt accaaaaagc accgcagtaa ggaaggtctg gttgagcgtt
     7921 tcgaattgta cgttaacgga aaagaaattg cgaattccta ttcagagctc aacgatccta
     7981 ttgatcagcg cgaccgattc atggagcaaa tggcattggg cgagcggggt gatgacgaag
     8041 cgatggtgat cgatcacgat ttccttcgtg cacttgaatt tggaatgcca ccaacggcag
     8101 gactgggtat tggtattgat cgcttgacca tgatgatgac caatcaggct agtattcagg
     8161 aagtactatt atttccgcaa atgcgtccgg agaaataatg ctttgatttt tgaattcccc
     8221 aaaaacccgg aaaattccgg gtttttgcat tattagcagc taaaaaagct aaattgtacc
     8281 gtccaaaccg gaatagaatg aaaaatttga ttttctctgc ttttgcagtt gcagtttcac
     8341 aattcacatt tgcacaaacc ttaagctgga gtgcatacac agattctact gtaacctatt
     8401 cttctccacg cgcagtagat cttacaggtg atggtgtgtt ggatatcata atgggtgccg
     8461 gaaaagaagc ggctgtaacc aactatggta tacttgcatt taacggcgca acgggcgcta
     8521 gtttatggac agtacaaacc cgcaatgaaa ttttcaccag tgcgcaattt cgggatatca
     8581 caggcgataa tattcctgat attttcctgg gaggaagaga tgcacaacta tatgccatca
     8641 atggaagtac aggcgctatt atttgggaat actggccttt tgccaatggt aatccaaatc
     8701 tgcagggatt gtataatttt tatacccccc agatcattcc ggatatgaac ggcgataata
     8761 ttcccgattt actgcttgcc aatggaggag atcacgccgc tccggtttgg caaaccaatc
     8821 gtccccccgg acatttgatg atcatcagcg caagcgatgg cgcattgata tcgatggtag
     8881 aagttcccga tggtgcagaa acgtattgct cacctgttgt agctaattta aaaggcgatg
     8941 gtgtattgta tgtggtattc ggcacaggcg gtgaaacatt acccggttca ttatgggttg
     9001 ctccactact cgatgttttc aatgaagatc tgagcagcgc tgttgctttg gctacacatc
     9061 ccacactagg atttgttgca cccccaacag ttgcccgttt tagttctgct gctcacatgg
     9121 atattgtcgc tcatggttat gatggtactt tgtatcggtt taaaggatca gactactctt
     9181 tggtatggag taaaagtttt cccggaacag aaaccagtgc ctctccagtg attgggaatt
     9241 ttacaggtaa tctgactccg gatgtttttg gtgttttata caaaggcgtt gcgccttctt
     9301 attcggattt ctatcaggta atgcttgatg gagaaaccgg caccctggtt tataaggata
     9361 gtcttttgtc tatgcatttt gctgctccgg ttgctttcga cagcaatggt gatggcagag
     9421 atgaggtgct gatttcagga tcaaatcatg tcggacattt tcgccatcag ctcatgctga
     9481 tcgatttcca aaacaacaca agcgcaaatt attggaacag cgaggcaggt agtaatatcg
     9541 gaagcactcc ctgggttgga gatctcgata atgatgatat gattgatctg gtatttgctt
     9601 tccgcaagga cagcctcaac ccaatgggct ggaaaggaac gtttgtgaag cgactcaata
     9661 ccggaatgaa tatgccgatt aaggacattg catggggtag ttatatggga tcggaatatg
     9721 acggccatta caattacacc ttcacgaatt gcggactggg cagtatttta tcgaatgtat
     9781 ttgtaagtaa tccaagttgt aatggtcttt cagatggaag catcaatcct gttcccggaa
     9841 acggaactgc acctttcact tttctgtggt cggacggcag cgtgggtgat aaacttctca
     9901 atgtacctgc cggacaatat tccctaattg ttaccgatgc aacagggtgt tcggagttaa
     9961 gaatgtttac gctttcaaac ccctatacca tttcctttgg cggaattaat ccggttactt
    10021 gtccgggtgg aaccaatgga acggcaacag taagttcaac cggttgttat tgcatgttca
    10081 gcggttgtaa ttttctatgg gaacaaggca caacaggata tacaatttca ggcgttccaa
    10141 caggaatgta caccgttacc atcacgcatc tcgatggatg tgttgtagtg gattctgtat
    10201 atattcccga aggacttcct gtactctcca ctactctggt tacacaaatt gattgctttg
    10261 gcgcagataa tggcacaata gaactcgtac ataattttaa tggaacaaat tatgtttgga
    10321 gcgatggatt taacggagga gttcacaccg gacttgaacc cggaaattat acctataccg
    10381 caacagatc
//
LOCUS       DAOMYG010000043        10253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2178_length_10253_cov_8.267994, whole genome shotgun sequence.
ACCESSION   DAOMYG010000043 DAOMYG010000000
VERSION     DAOMYG010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10253
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2178_length_10253_cov_8.267994"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1817
                     /locus_tag="PK610_02425"
     CDS             90..1817
                     /locus_tag="PK610_02425"
                     /inference="COORDINATES: protein motif:HMM:NF023504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HRJ34808.1"
                     /translation="MAGGKETPRQKMIGMMYLVLTALLALNVSKEIVIAFITLNDKLD
                     QSTMSIEYKNDMVYQNLDRMYNVAETREKVKSWREKALRISALANDAHNFYLEETNRL
                     IKAVEGEGKEWIIEEGGKRKLANLMDIGSKDDYDAATRLFVGNANNIVEEGKQLKRKM
                     HLYRDSLCYLVSSYEFNGKNFAFIPEYNGNTFSEEVLNAALTGANPEDTSKIKQLYKI
                     LTLPETMKNHEEEVPWQAGMFDHAPVVAAAAMFTSLRNDVRTAESIALEHLLTKVNVP
                     VFNFNKIEPLAFARTSYLNVGDSMNLNVMIAAFDSNEVAKIRFAVNDTNKANYISHTG
                     TISVKGNRPGAYMMSGLVAVKENGVEKWKPWSYSYEVGEPTGVIANEDLTVIYAGYDH
                     TFSASVSGYPQDRVSLSVPGVKTTPKGGGKFSVVAPASLINKKIKASITVRTDGGTTK
                     SFSGPEFTVKPLPIPTSYLGNISANQGDVSITELKNNAQYLRVAYDPSVTLDPSKVKF
                     TVKSFEMSVMVNGNSRKFQSNSAQLNQQMTDFIKIAPPGTLVSFVGISVQGPAGPIRV
                     PNMTLRVKP"
     gene            1926..3845
                     /locus_tag="PK610_02430"
     CDS             1926..3845
                     /locus_tag="PK610_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRJ34809.1"
                     /translation="MVGVNGISVHFGDRVLYDELSFMVNKEDRIGLTGRNGAGKSTLL
                     KIFAGIQKPDSGSISVPKGLTIGYLPQEMQHKEDAEIFEEAKSALAEFNEVTNKLDQL
                     NDELVTRTDYESEEFGEILNLISDLNERLNMIGAGNVDEDVERVLFGLGFERTDLTRK
                     MSEFSGGWKMRVELAKILLQRPELLLLDEPTNHLDIESIQWLEDFLISHRGAIILISH
                     DRRFLDAVTKRTVEISNGKAYDYKASYSKYVELRRQEIELQRNARKNQEKTIKETEQL
                     IDKFRAKASKAAFAQSLMKKLDRMEKIEVDDFESAKINIRFPPAPHSGKVVLETQSLT
                     KTYGDKTIFKNLDLIIGKGEKIALVGKNGAGKTTLTKLITGQEKFEGEIRHGHQVSIG
                     YFAQNEAEKLDRNKTVFETIDDEAQGEIRKSVRGLLGAFLFGGDDIEKKVSVLSGGEK
                     TRLALCKLLLSPVNFLILDEPTNHLDLASKEVLKNALKNYDGTLLLVSHDRDFLSGLT
                     SRIFEMKDQKVSVHHMSVDEFLQMKKNASIADYEYQKAQAKKENQVKGSASNNQREQD
                     KDKKRLQGRLEKLEQEISKKEEQLKKAEKELADADYSDSTRLASLTSGYDAIKSQIDA
                     AYIEWETLQAQIAEL"
     gene            3944..5761
                     /locus_tag="PK610_02435"
     CDS             3944..5761
                     /locus_tag="PK610_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34810.1"
                     /translation="MKKNVLLLFTLSLFGQISAQSVNTPIEGATEFVKNSGFRITPPL
                     RDLAELIPEFDERDDHRIIHNNRLAFPAANPDALPKGVDPIAQFNMGERMFQQRAPLV
                     QWNGMNGGTPPDPSGAAGPNHYVQAVNTSWRVYNKTGGTLTASANLSSLWSGSSNSGD
                     PIVMYDRHADRWFISQFQTGNNAILIAVSTTNDPTGTYYAYSFIPVASQFPDYPKFSI
                     WHDGYYMTANYSTKRIVVFDRAKILVGDPTAGMLIRNMPSTPSNGFFCALPADADGQL
                     PPAGTPCPVFSFEDDAWASGSADRIRVYNVSCDWSALTIDFTLNQLLPAQPFNSVFNA
                     SWNDIEQPGTTQKLDAIAGVLTYRAQYLRWTGYNTVTLCHVVKVDATTGQAGIRWYEL
                     RQNQSSGTWSIYQEGTYAPTNESRWCGSIAMDEQGNIGMAYAVSGSSTFPSIRYTGRL
                     ATDPLGQMTFIEQVGATGTSVQTGVNRFGDYSQTSLDPDGLTFWHTGEYISAGRKTRI
                     FSFRIAGAAGVDEQVIGEIKVFQDGMDLIVDAHSLLNDRETHVDLFDIQGRTISEKVV
                     LPVQSAFNTSINVSGLAAGPYLVRIGNEHYQRVIKVMVQ"
     gene            5778..6098
                     /locus_tag="PK610_02440"
     CDS             5778..6098
                     /locus_tag="PK610_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34811.1"
                     /translation="MKRILTIMILALLAVACKNSKAVSETSNEEQIIGTVSHKYRAGG
                     CPTVIIAPNGGDTLTLIPTQSLGKFDIDGLEVRFTYHPLRRPNPEGCVVGIPASIVGI
                     AKAK"
     gene            6172..7494
                     /locus_tag="PK610_02445"
     CDS             6172..7494
                     /locus_tag="PK610_02445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRJ34812.1"
                     /translation="MKPRIFRTLVLLATFSLIGIVVLQVYWVNSTLKIQENQEILLRK
                     EYSQQEKIFNDRVTIALTNVAEQIMDYYDDHSDLYNAVEQKQSNYFIVRMNDTLHPYL
                     LERMLRMEFDQNNIKEDFEYGIYDCFSDSLRYGKYIDMDASNSNDVSQRPQLKMANDG
                     HYFSVFFPNRVGFTPHQVDEPLGAWFYTSIIILIVTGFFGYSLYVIMHQKRLADVKND
                     FINNMTHELKTPISTISISSEVLLKDGICNDPQRVNQYARIIYNENKRLENQVERVLQ
                     IATLDKEEISLKKTNIHIHDLLESCVSNFRVPIEANGGSLSLELNAATDEIEADKVHL
                     TNIFYNLLDNARKYSEGSPEVIVRTESDANGVRIKIIDHGIGIAKDALKNIFEKFYRV
                     PTGNVHNVKGFGLGLYYVKVMTEQHGGTISVKSHPGKGSEFELYFPFH"
     gene            7505..8200
                     /locus_tag="PK610_02450"
     CDS             7505..8200
                     /locus_tag="PK610_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ34813.1"
                     /translation="MTDKKATLLLVEDDMNLGFVVQDNLKMSGYKVVLATDGKQGLKQ
                     FNEGKFDMCLLDVMLPHKDGFSLAEDIRKANAEIPIIFLTAKSMTEDKIKGFRSGGDD
                     YITKPFSTEELLLRIEAILKRSKPGRSVQKDHFAIGNYSFDASNYTLKLGGTEQRLTK
                     KESEILKLLCAHINEVLPRELVLNMIWGDDNYFNGRSLDVFLSKIRKYLKDDPSVSIT
                     NVHGVGFRLQVAS"
     gene            8210..8728
                     /locus_tag="PK610_02455"
     CDS             8210..8728
                     /locus_tag="PK610_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34814.1"
                     /translation="MKSLYYFLIALIFVSCGETQWERTTFPSGFSLEKPDYMSISEAI
                     KPDAEIQLENVNRELYYVMDVRPKSELEQAGVMFDLKAFSASLRSSMTEEMKSPWEED
                     LGALKCGDLQAQHFQVGGKSKFGPKVSYLVMVVDAGDDFLIQTAWTPSAQKTSWFNDD
                     MKRIMTSLQRNH"
     gene            complement(8725..9462)
                     /gene="lptB"
                     /locus_tag="PK610_02460"
     CDS             complement(8725..9462)
                     /gene="lptB"
                     /locus_tag="PK610_02460"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRJ34815.1"
                     /translation="MKLWTREIFKQYKDRVVVNGVSVEVNQGEIVGLLGPNGAGKTTS
                     FYMIVGLIKPDKGSVFLDNEEITKEPMYRRAQKGIGYLPQEVSVFRKLSVENNILAVL
                     ETRKLNKKQRNDRLEELLDEFSLQHVRKNLGIQLSGGEKRRTEIARALASDPKFILLD
                     EPFAGVDPIAVEDIQSIVAQLKNKNIGVLITDHNVQETLSITDRAYLLFEGKILKQGT
                     AEELAEDPVVRKVYLGQHFVLRRKTYN"
     gene            complement(9467..>10253)
                     /locus_tag="PK610_02465"
     CDS             complement(9467..>10253)
                     /locus_tag="PK610_02465"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRJ34816.1"
                     /translation="YFPGLNNMVLFRRRAISQLPDTNLYQKQISPTEIEYLKNVNITE
                     FTYYLRYAKNEKFFSGEFDRVSMGTRKPIYEFQYAIGIKDLFGGDYNYHKLVGSITQW
                     FPIGTLGWGRYRFEAGKFWGTVPFPVLEIHRGNETYYCDEQAFNTMLYLEFVSDQYFS
                     AFYTHHFDGFFLNHLPLLRRLKWREVAAIKACWGTITEANKREVQFPTYLYNFSNVPF
                     LEASVGVENIFKFLRIDFIWRMTYLDHPNIAKFGLRAKFDVDF"
BASE COUNT         3118 a         1883 c         2320 g         2932 t
ORIGIN      
        1 ggttgggtgg ttcaatatgc tttgcgctct ctgttacttt ccttttgttt ttgaattgta
       61 ggttcatact aactcaaaaa cgaaacatta tggcaggagg aaaagaaacc ccaaggcaaa
      121 agatgatcgg tatgatgtat ctggtgctta cagcattgct tgcgctcaat gtgtccaaag
      181 aaattgtgat tgcattcatc acattgaacg ataagctcga tcaatctact atgtcaatag
      241 aatacaaaaa cgatatggtg tatcagaatc tcgaccgcat gtacaatgtt gcggaaacac
      301 gggaaaaagt aaaatcctgg agggaaaaag cactccggat ttctgcgctg gcaaatgatg
      361 cgcacaattt ttacctcgaa gaaacaaacc gattgatcaa ggcagttgaa ggggaaggaa
      421 aagagtggat cattgaagaa ggtggtaaac gaaagctggc caatttaatg gatattggat
      481 caaaggacga ttatgatgct gcaacacggt tatttgtggg aaatgccaat aatattgtag
      541 aagaaggaaa gcagcttaaa cgaaaaatgc atctctaccg cgattccttg tgctacctgg
      601 tttcttctta tgaattcaac gggaaaaact ttgcctttat tccggaatac aatggcaata
      661 ctttttcaga ggaggtatta aatgcagcgt tgacaggagc aaatccggag gatacaagta
      721 aaattaaaca gttgtataaa atactgactt taccggaaac aatgaaaaac catgaagaag
      781 aagttccatg gcaagccggc atgtttgatc atgctccggt agttgcagca gcagcgatgt
      841 ttacttcact acgcaacgat gttcgcactg cagaatccat agccttagaa catttgttga
      901 caaaagtaaa tgtcccggtt tttaatttta ataaaataga acccttggca tttgcacgga
      961 ccagttattt gaatgtgggc gattcaatga atctgaatgt gatgattgcc gcatttgact
     1021 caaacgaagt ggcaaaaatc agattcgcag ttaatgacac caataaggcg aattacatca
     1081 gtcataccgg aacaatttct gtaaaaggaa acaggcccgg tgcatatatg atgagtggtc
     1141 tggttgcagt gaaagaaaat ggagttgaaa aatggaaacc ctggagctat tcctatgaag
     1201 ttggggaacc aaccggagta attgcaaacg aagatctgac agttatttac gcggggtacg
     1261 atcatacgtt ttcagcgtcg gtttcaggtt atccgcaaga ccgtgtttcg ctttctgttc
     1321 ccggagtgaa aacaacacca aaaggcggcg gtaaattttc tgttgtggct ccggcaagtt
     1381 taatcaacaa gaaaataaaa gcatctataa cagtacgtac tgatggtggt acaaccaaat
     1441 catttagcgg gccagaattc actgtgaaac cacttcccat accaacatct taccttggca
     1501 atatcagcgc aaatcaagga gatgtttcga tcacggaact aaagaataat gcacaatatt
     1561 tacgggttgc ttatgatccc agtgtaacac ttgatccttc aaaagtgaag tttacagtaa
     1621 aatcattcga aatgtctgta atggtaaatg ggaacagtag aaaatttcaa tcaaacagtg
     1681 cgcaattaaa tcagcagatg accgatttta tcaagattgc accgcccggc accttggttt
     1741 catttgtagg aatcagtgtg cagggaccgg caggtccgat tcgtgttccg aatatgacat
     1801 taagggttaa accctaagat gttttggtga acatttgcaa ataaagcaca tcggcgagac
     1861 gaaagctggg gtagtttacc tgagctttcg tcttttttta agcggcgaat gattattttc
     1921 gcgccatggt aggagttaat gggatttcgg ttcattttgg agaccgtgtg ttgtatgatg
     1981 agttgtcctt tatggtcaac aaggaagatc gaattggtct aaccggaaga aacggtgcag
     2041 gtaaatccac actgcttaaa atttttgcag gcattcagaa gcctgacagc ggatccattt
     2101 ccgttccgaa aggtttgaca atcggatatc ttcctcagga aatgcagcac aaggaagacg
     2161 cagaaatttt tgaagaagcg aaatcggcat tggctgagtt taatgaggtg accaacaaac
     2221 tcgatcagct caatgatgag ttggttacac gtacagatta tgaatcggaa gaatttggtg
     2281 agattttaaa tcttatcagt gatctgaatg agcgattgaa catgattggt gcgggaaatg
     2341 ttgatgaaga tgtagagcgg gtgctttttg gtttgggatt tgaacgcact gatctgactc
     2401 gaaagatgtc ggaatttagt ggtggttgga aaatgcgggt tgagcttgca aaaatattat
     2461 tacagcgtcc ggaactattg ctattggatg aacctacgaa tcacctggat attgaatcca
     2521 ttcaatggct ggaggatttt ttgatttcgc ataggggagc aattatatta atttcgcacg
     2581 accgtcgctt tcttgatgcc gtaactaaac ggacagttga aatttccaac ggaaaggcat
     2641 acgattacaa ggcttcttat tcaaagtatg ttgaattgcg cagacaagaa atcgaattgc
     2701 agcgcaatgc acgtaaaaat caggaaaaaa cgattaaaga gacagagcaa ctcattgata
     2761 aatttcgtgc aaaagccagt aaggcagcgt ttgcacaatc gttaatgaag aagctcgatc
     2821 gaatggagaa aatcgaggtt gatgatttcg agtcagccaa aataaatatc cgattcccgc
     2881 ctgcgccaca ttccggaaaa gtagtattgg agacacagtc gcttacaaaa acttacggtg
     2941 acaaaactat ttttaaaaac cttgatttga tcatcggaaa aggtgaaaaa attgccttag
     3001 ttggaaagaa tggcgcaggt aagacaacac ttacaaagtt aatcaccggt caggagaaat
     3061 ttgaaggaga aattcgtcat ggccaccagg ttagtatcgg gtattttgca caaaatgaag
     3121 ctgaaaaact ggaccggaac aagacggttt ttgaaaccat tgatgatgaa gcacaaggtg
     3181 agatcagaaa aagtgtgcgt ggtttattag gggcgttttt gtttggtggt gatgatattg
     3241 aaaagaaagt aagtgttctt tcaggtggag agaaaacacg gctggcgttg tgtaagttgt
     3301 tattgtcgcc ggttaatttt ctgattctcg atgagcccac caatcacctt gaccttgcca
     3361 gtaaggaagt gctgaagaat gcactgaaaa attacgacgg aacactcttg ttggtttcgc
     3421 acgaccgcga ttttctttcc ggtcttacaa gcagaatctt tgaaatgaaa gatcaaaaag
     3481 taagtgtgca tcacatgagt gtggatgagt tcctacagat gaagaagaat gccagtattg
     3541 cggattatga ataccaaaaa gcacaggcga agaaggaaaa ccaagtgaaa ggtagtgctt
     3601 cgaataatca gcgtgaacag gacaaggata aaaaacgttt acaaggcagg cttgaaaaac
     3661 tggagcagga aatcagcaag aaagaagagc agctgaaaaa agcggaaaag gaacttgctg
     3721 atgctgatta ttcagacagt acacgattag catcattaac ttccggatat gatgctatta
     3781 agtcacaaat cgatgctgcc tacattgaat gggaaaccct acaggctcaa atagcagaat
     3841 tgtaacgccc gtttcctcag cattactttg cgtacgctaa tctaataccg taatttttgt
     3901 gattggggtc ttttttcgtt ataattacga aacaatactt gctatgaaaa agaatgtgct
     3961 tctgcttttt acgctgagtt tatttggtca gataagcgcg caatcagtta acaccccaat
     4021 cgaaggcgct actgagtttg tgaaaaattc aggatttaga ataacaccgc cgttgagaga
     4081 tttagcggaa ttgattcctg aatttgatga aagagatgat catcggatta ttcacaataa
     4141 ccgacttgca tttccagcag caaaccctga tgcacttcct aagggcgttg atcctatagc
     4201 acaatttaac atgggggaac gtatgttcca gcagcgcgct ccattggttc agtggaatgg
     4261 aatgaatggc ggaacccctc ccgatccctc cggcgcagcc gggccaaatc attatgttca
     4321 ggctgtaaac acgagctgga gagtttacaa taagacagga ggaacgctga ccgcctccgc
     4381 aaaccttagc agtctttggt cgggcagttc aaattccggt gatccgattg taatgtacga
     4441 tcgccatgcc gaccgatggt tcatcagcca gtttcaaacc ggaaacaatg ctatcttaat
     4501 tgctgtttcc acaacaaatg atccaacagg aacttattat gcatattcgt ttattccggt
     4561 ggcctcgcag tttccagatt accctaaatt ttcaatatgg catgatggat attacatgac
     4621 agccaattat tcaactaaac gtatagttgt atttgatcgc gctaaaatat tggttggaga
     4681 tccgacagca ggaatgctta ttcgcaatat gccttctaca ccttccaatg gttttttctg
     4741 tgcgcttcca gcagatgctg atggtcagtt gcctcctgcg ggaactcctt gtcctgtttt
     4801 ttcgttcgaa gatgatgcat gggcttccgg aagcgcggat cgcattcgtg tttacaatgt
     4861 aagttgcgat tggtccgcac ttactattga ttttacgctc aatcaacttc tccccgcaca
     4921 gccgtttaat tcggtgttta atgcttcgtg gaatgatatt gaacaacccg gtactacaca
     4981 aaaacttgat gccattgccg gagttctcac ctatcgggca cagtacctgc gttggacagg
     5041 ttacaacaca gtaacacttt gccacgtagt aaaggttgat gcaacaaccg gtcaggccgg
     5101 aattcgctgg tatgagctta ggcaaaatca atcttcggga acatggtcca tttaccagga
     5161 aggcacttat gcaccaacaa atgaaagccg atggtgcggt agtattgcaa tggatgagca
     5221 gggtaatatc ggaatggcct atgctgtttc aggttcaagt acttttccca gtattcggta
     5281 tacggggcga ttggcaacag atcctctcgg gcaaatgact tttattgaac aggttggtgc
     5341 taccggaacc tcagtgcaaa caggtgtgaa tcgttttggc gattattctc aaacatcact
     5401 tgatcctgat ggcttgactt tctggcacac cggagaatat atttctgccg gacgaaagac
     5461 acgtattttt tcattccgaa ttgcaggagc agccggggtt gatgaacaag taatagggga
     5521 aattaaagta tttcaggatg gaatggattt gattgttgat gcgcatagtc tgctaaatga
     5581 tagggaaact catgtagacc ttttcgatat tcagggaaga accatcagtg aaaaggtcgt
     5641 tttaccggtg cagtctgcat ttaatacatc gataaatgtt tccggtcttg cagcgggtcc
     5701 ctaccttgta aggattggaa atgagcatta ccaaagagta attaaagtaa tggtacagta
     5761 attgattatt tgcttttatg aaacggattt taaccattat gattctggcg ctgcttgcag
     5821 tagcgtgcaa aaactcgaaa gctgtgagtg aaacatcaaa cgaagaacaa attattggaa
     5881 cagtatccca caaatacaga gctggaggtt gtccgacggt tatcattgcg ccaaatggtg
     5941 gtgatacatt gacattgatc cctactcaat cacttggtaa gtttgacatc gacggactag
     6001 aggtacgatt tacctatcat ccgttgcggc ggccaaatcc ggaagggtgt gttgttggaa
     6061 ttccggcttc aatagttgga atagcaaagg caaagtaaaa aactgttagc gacttgttaa
     6121 gccctgcatc tgcagggctt ttttcgttat tttgtgtgct taatactttc aatgaagccc
     6181 agaatattcc gaacgcttgt attacttgct actttttcgt taataggaat tgtggtttta
     6241 caggtgtatt gggttaacag tacattgaaa attcaggaga atcaggagat tcttttgcgc
     6301 aaagagtatt cgcagcagga gaaaattttt aatgatcgtg taacaattgc gctcaccaat
     6361 gtcgcagaac agatcatgga ttattacgac gatcattctg atctgtataa tgctgttgaa
     6421 caaaagcaat caaattattt cattgtacga atgaatgaca ccttgcatcc ctacctttta
     6481 gagcggatgc ttcggatgga gtttgatcaa aacaacatca aggaagattt tgaatacggc
     6541 atttatgatt gtttttctga ttcattgcga tatggaaagt atatcgacat ggacgcatcc
     6601 aattccaatg atgttagcca gcgtccgcag ttaaaaatgg caaatgatgg tcattatttt
     6661 agtgtgtttt ttccaaacag ggtaggcttt actccgcatc aggttgatga acctctgggt
     6721 gcctggtttt atacttcaat tattattttg attgttaccg gtttttttgg gtattccctt
     6781 tatgtgatta tgcaccaaaa gcggttggct gatgtaaaga atgatttcat caataatatg
     6841 actcatgaat tgaaaacacc cataagcacc atttcaattt cgagtgaggt tctacttaaa
     6901 gacggtattt gtaatgatcc ccagagggta aatcaatacg caaggattat ttacaacgaa
     6961 aacaagcgtt tggaaaatca agtggagcgg gttttgcaaa tcgcaacgct ggataaagaa
     7021 gaaatatcat tgaagaaaac gaatattcat attcacgact tacttgagag ttgtgtttca
     7081 aatttcagag tgccaattga ggcaaacggc ggatcgttgt ctctcgaatt gaatgctgca
     7141 acagatgaaa ttgaagcgga taaagtacat ctaacaaata tcttctacaa tttgctcgac
     7201 aacgcccgga aatattctga aggatcgccc gaagtaattg ttcgtacaga aagtgatgct
     7261 aatggagttc gaataaagat catcgatcat gggattggga ttgcaaagga tgcattgaaa
     7321 aatatttttg aaaaattcta ccgcgtacca accggaaatg ttcacaatgt aaaaggattt
     7381 ggattaggtc tttactatgt gaaagtgatg acggagcagc atggcggcac aatttcagtt
     7441 aagagccatc caggcaaggg cagtgagttt gaattgtatt tcccttttca ttaacaatac
     7501 gattatgaca gacaaaaaag ctacactgtt gctggttgaa gatgacatga atcttgggtt
     7561 tgtggtccag gataatttga aaatgagcgg atacaaagtt gttctggcaa ctgatgggaa
     7621 gcaaggtctt aaacaattta atgagggtaa gtttgatatg tgtctgcttg atgtaatgtt
     7681 gcctcacaaa gacgggttta gtcttgccga ggatattcga aaagccaatg ctgaaattcc
     7741 gattattttt ctcactgcaa aatccatgac agaagataaa ataaaagggt ttcggtcagg
     7801 tggggatgat tatattacca aaccattcag cacagaggaa ctattgcttc ggatcgaggc
     7861 aattttgaaa cgcagcaaac cgggacgatc ggttcagaag gatcattttg caattggcaa
     7921 ttattcgttt gatgcatcca attacacact taaacttggg ggaacagagc aacgcctcac
     7981 aaaaaaggaa tctgaaatat tgaagttact ttgtgctcac ataaatgaag ttttaccacg
     8041 ggaattggta ttgaatatga tttggggaga cgataattat tttaatggcc gtagccttga
     8101 tgtgtttctt agtaagattc gcaaatacct aaaagatgac ccgtctgttt ccataactaa
     8161 tgttcatgga gttggttttc gcttacaggt tgcatcttaa tttgttggta tgaaaagttt
     8221 gtactatttt ttgattgcat tgattttcgt ttcctgcgga gaaacgcaat gggaaaggac
     8281 aacctttcca tccgggttta gtcttgaaaa acctgattac atgagtattt cagaggcgat
     8341 caagcctgat gctgaaattc aattggaaaa tgtaaacagg gaattgtact atgttatgga
     8401 tgtacgcccc aaatctgaat tggaacaagc gggcgttatg tttgatttga aggctttttc
     8461 tgccagtttg agaagctcaa tgacagaaga gatgaaaagt ccctgggaag aggatctggg
     8521 agctttgaaa tgcggtgatt tgcaggcgca gcattttcag gttggaggaa aatcaaaatt
     8581 tggacctaaa gtaagttatc ttgtgatggt agtagatgca ggagatgatt ttctgattca
     8641 aacagcttgg acaccttctg ctcaaaagac gagttggttt aatgatgaca tgaaacgcat
     8701 catgacatca ttgcaacgaa atcattaatt gtacgttttg cgtctcaaaa caaagtgttg
     8761 tccgagatac acttttcgca caacgggatc ctctgccagt tcttcggctg tcccctgttt
     8821 cagaattttt ccttcaaaca acaggtatgc tctgtcggtg atagataatg tttcctgaac
     8881 attgtggtcg gtaataagaa ctccaatgtt cttatttttt aattgcgcaa caatactttg
     8941 gatatcttca acagcaatgg gatccacgcc ggcaaatggt tcatcaagaa gaatgaattt
     9001 aggatctgat gccagggcac gtgcaatttc agttcttctt ttttctcctc cggaaagttg
     9061 aattccaaga ttttttctca cgtgttgcaa tgaaaactca tccaataatt cttctaaccg
     9121 atcattccgt tgtttcttgt tgagttttct ggtttctagt acagccaaga tattgttctc
     9181 aacgcttaat tttctgaaaa cagaaacctc ctgcggaaga tatccaatgc ctttttgtgc
     9241 acgtctgtac ataggttcct ttgtgatctc ttcattatcg agaaaaacgg agcctttatc
     9301 aggtttgatc aggccaacaa tcatgtaaaa ggaagttgtt tttcctgctc catttggacc
     9361 aagcaagcca acaatttcac cttggttgac ctcaacggaa acaccgttta caaccactct
     9421 gtctttgtac tgtttgaata tttctcttgt ccagagtttc ataggcttaa aagtcgacat
     9481 caaatttagc gcgtaatccg aacttagcga tgttggggtg atcaagatag gtcattcgcc
     9541 agataaaatc gatacgtaga aacttaaaga tgttttctac accaacggaa gcttccagga
     9601 aaggcacatt gctgaaatta taaagatagg ttgggaattg aacttctctt ttatttgcct
     9661 cagtaatggt tccccaacat gctttaatag cggcaacttc tcgccatttc aatcggcgaa
     9721 gcaaaggaag atggttgagg aagaaaccgt cgaaatgatg ggtataaaat gcgctgaagt
     9781 attgatcgct tacaaattcc agatagagca tagtgttaaa cgcctgctca tcgcaatagt
     9841 aggtttcgtt tccgcgatgt atttccaaaa caggaaaagg aactgtaccc cagaattttc
     9901 cggcttcaaa acggtaacgt ccccatccta aagtaccaat tggaaaccac tgtgtgatac
     9961 ttccaacaag tttatgataa ttgtaatccc cgccaaaaag atcttttatt ccaattgcat
    10021 attggaattc gtaaataggc ttacgggtcc ccatactcac gcgatcaaat tcgccggaga
    10081 agaatttttc attctttgcg taacgcagat agtaagtgaa ttcggtaata ttaacgtttt
    10141 tcagatattc aatttctgtt ggactgattt gtttctgata gagattggtg tcaggtaatt
    10201 gagaaatcgc tcttcttcgg aatagcacca tgttgtttag tcccgggaaa tat
//
LOCUS       DAOMYG010000044        10242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2182_length_10242_cov_9.128006, whole genome shotgun sequence.
ACCESSION   DAOMYG010000044 DAOMYG010000000
VERSION     DAOMYG010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10242
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2182_length_10242_cov_9.128006"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1925)
                     /locus_tag="PK610_02470"
     CDS             complement(<1..1925)
                     /locus_tag="PK610_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009088787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRJ34817.1"
                     /translation="MYNKFIERPVLSLVISIFFVLLGLLALVGLPVTQYPDIAPPAVT
                     VTTRYTGANAEVCTKAVVTPLERAINGVPGMTYMSSVSGNDGTSIITVFFEVGTDPDF
                     AAVNVQNRVATVLDELPEEVIRAGVSTEKEVNSMLLFINLISSDTTLDESFIFNFADI
                     NVLPELKRIDGVGFAQILGSKEYAMRVWLHPERMEAYAISADDVVERLREMNLEAAPG
                     KVGEGSGKTAQSMQYVLKYTGKMTEQSQYENVVLRVDADGQLLRLKDIADIEFGAQTY
                     SVLSKENGRPSAAIMLKQRPGSNASEVIANIKDRLIFLKETQFPPGMSYTLSYDVSRF
                     LDASIHEVIKTLIEAFILVALVVFIFLQNLKLTIISILAVPVSLIGTFAFMQLFGFSL
                     NLLTLFALVLAIGIVVDNAIVVVEAVHAKMHEKHLKPIAATKEAMQDIGSAILAITLV
                     MSAVFVPVAFLSGPVGVFYRQFSITMAISIVISGVTAITLTPALCALMLRESDAHETN
                     NQLLRRFFRWFNSSYDKLSGRYVRAITSIVNRRVVTFGVLIGFIVFIYFIGSVLPSGF
                     IPTEDQGTLYVNVTTPPGATLERTEEVMNEIDKIAKELSEVESISSMSGYSMMTEGIG
                     ASFGMSTINLKPWDERS"
     gene            complement(1936..3039)
                     /locus_tag="PK610_02475"
     CDS             complement(1936..3039)
                     /locus_tag="PK610_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRJ34818.1"
                     /translation="MKALQFFVLSAILFSCSNQEEVKDTETFSVTSPIVLDTSLATSY
                     VGEVTAIQNVEIRSRVNGYISANHIDEGKMVSQGQLLFSIDNSIQREELAKASAILKS
                     CILEAKTAEIDYKNTKQLVEKNVVSPTELELSKIRYESAQAKVEEAQANETHARTILS
                     FTEIRAPFSGIISRIPFKLGSLVEEGAMLTTISNLDQVFVYFSFTEREYLNYLQSNSD
                     TSKSVRFVMANGELFPHTGVIETTAGEVSTGTGTITFRARFPNPDRLLRHGATGKIQL
                     FSSFSGVMLIPQKSVFEIQDKKYVYVLNEEGLVKSRVITVEKEIPDFYIIEKVLDTKD
                     KIVYEGAQLLREDTKINGQFVSMRDILKDLSKN"
     gene            complement(3108..3392)
                     /locus_tag="PK610_02480"
     CDS             complement(3108..3392)
                     /locus_tag="PK610_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34819.1"
                     /translation="MQFAYILSSIFISDFNNAEAFIEEITTEETNSGNDPCADCFTES
                     EDASDLKLFLKDGQSFISVKREGAQATHSILSLRKGKGYPDSLFSPPDFC"
     gene            complement(3509..4060)
                     /locus_tag="PK610_02485"
     CDS             complement(3509..4060)
                     /locus_tag="PK610_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34820.1"
                     /translation="MISLLTRFSRRKITEDTLATAFVSNLLEVVEKAFPDVAGLINED
                     PDFPKSPGIELSDDEQFLMIVLVGNIRLLSESFDPEMEDTLKDSIFDKCAYSMGMDRN
                     EFIAIYKEYSDFLSRVNHPSKNILYSMARGVFYKYNLNHYQADYFRTLNTPNPIFLKR
                     MNEVMNYFIWDWQAFFEKYKVVL"
     gene            4280..5404
                     /locus_tag="PK610_02490"
     CDS             4280..5404
                     /locus_tag="PK610_02490"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRJ34821.1"
                     /translation="MKTRHLVLAGFLSMLAFMTWTLIKNKDIFSLSGEKPKDAGTRYV
                     DVISSKADSTEVLIEGQGRISSSRRIDVSSEVQGLLLPGKILKPGTTVRQGEQLFQVR
                     DQDARLALQARKSNYMNILANALPDIRLDFSDVYPDWKNFFDQLDAEKPLPELPQPKT
                     GREKSFLASKNIFGEYYTIRAEEERIRKYSIIAPFDGTITDVFAEPGSVVNPGTRLLS
                     IIKGSDLEIELPIDVKNIGWVKMGAEVQLKATSGPGEFKGTVIRIGEFVNANTQSVPV
                     YIRINKQNGDALYTGMYLTGYIHAGKIPGTISIPRRALFEDNHCFEVKDSVLLKRQLQ
                     IAFVTRESVIVSGIPNGTLLVAEPLTNVSDSMRVTPIVKQ"
     gene            5401..8928
                     /locus_tag="PK610_02495"
     CDS             5401..8928
                     /locus_tag="PK610_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRJ34822.1"
                     /translation="MRRLISFFITNPIWGNAFIFLTIVFGLLALSNMRRSFFPEMPPR
                     VITIDVAYPGASPVEMEDGVTIKIEQALEGLSGIKKITSSSEENRASLKIEAFEDTDM
                     DDLLTDVENTVNSINSFPVGAEKPIIRKLKVGSMGGTAAFLSLTGPDDLWKLKAKSEQ
                     IKNDFLAEKSISQIRVVGYPEVEISIEVKESKLLEYGLRFDQLTSAVRTNNLDLTGGT
                     IKTEEEEYIIRMRARETLADEIGKIVIRATPGGEIIRLSDIATVNYQFADRPYKSYID
                     GKRNINVIVSKLPEEDLGAIAKFITDYTDKFNEENEDFELRIIFQFADMLQERIDLLT
                     NNRIIGFFLVMICLGFFLSLRLSFWVAIGIPISFLGLFIAGYLYGMTINMISLFGMII
                     VIGILVDDGIVIAENIFAHYERGKSPMLAALDGTAEVFKSVIASVLTTVVAFSLLMYV
                     GGQFEMMREMAFAVVACLLFSLFEAILTLPQHLGHDSMLKPVKWGFYQKIRSRINKWI
                     DALRDGYAWLLGRIMSKHRAAIFIPVIFISTIIFLFKSEIIKFTFFPNIQFDDITVSV
                     AFKPGEREDQTEEFLEYCQEKVYEVRDSLEKHYGEPIITATTLSVGMAEFLDETGAHA
                     GNLRIQIDIEGKDIESFTIAELIRKKIGPITSPEKFTIGGEGRFGKPVALMLSSKNQD
                     ELLKAKEFVKDKFIKLGPLKDVNDNSPLGSQEIHIDLLPKAYLLGLSRSEIARQIRQG
                     FFGEEAQRLIIGEDEVRVWVRYPREDRQYLGQIEEMRIKTLTGETYPLRELVNFSIQR
                     GEVGIRHYNGKKEITVEADLVNANAPVGDAIELVTSKVIQPLNAQFPNVETNFLGQAE
                     RARETQGPLMTVLVLTVLLVILILALNFGSAMQAFLVLTAIPAGIFCAFLGHGLEGKP
                     VSLFSFWGIIALIGILVNDAVVMVDTYNRYIKNGMSVKDAAYEAGRSRFRAVLLTSLT
                     TVVGLYPLILEDSFQAQFLIPMAISVAHGLLWGTMLTILFFPSVIIFWNDVRRILYWF
                     KGNTPLVFAMLFLIITLALNIAIVFSPHLTGSVKMSAGIIMGAIIIQTLILLPFFIWK
                     PETAHRLLAIHAAIILNLVLIALPTGYHILFIFPVGAFLYLGYEKTLRWIFKFHSDTF
                     PKAESVEPVLRKNKLETQLANDIVNAKDI"
     gene            8912..10219
                     /locus_tag="PK610_02500"
     CDS             8912..10219
                     /locus_tag="PK610_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRJ34823.1"
                     /translation="MQRIFKLSFIFLPLFATAQQKLSLSDAIRIALEKNYKVEIARDQ
                     ISIAKNNNNWGEAGRWPSVDLQITQNNNILDQSNNPTAFIRDKIITNSVNGTLSANWM
                     LFGGFRVQFTKERLEQLQAQSEGNAILVVENTVQSVILGYYNAVLQKEKLDVLRKLIK
                     LSREKYEHMERKKDLGVSGTFDLLQIKNAYLSDSTSFLLQELAYRNSIRNLNLLMNQD
                     VETFYDLTDFLEVRTDRYDFDTLKRRMLRNNTTILNQYINLELQRTQTDLARSALYPV
                     VSLQMGTANSFSHFQTSMFESNGSNLNYYANFVVNFNLFNGGKTRRAIENSRIQEKIQ
                     QTSLDEQVFTLTSQLRNAYETYVARQVIMNLSTESYRSAEFNLRLAQERFDQGTISSF
                     NYRDVQLQFFNAAMQRLDAANTLLGSHTELLRLTGGIIDLNPN"
BASE COUNT         3123 a         2167 c         2033 g         2919 t
ORIGIN      
        1 aatgatcttt catcccatgg cttcaaatta atggtgctca ttccaaaaga agcgccaata
       61 ccttcggtca tcattgaata ccccgacata cttgagatgg attctacttc actcagttcc
      121 tttgcaattt tatcaatttc attcatcact tcttccgtac gctcaagtgt agcacccgga
      181 ggagttgtca cgttaacata caacgttccc tgatcttctg tcggaataaa tccggacggc
      241 aaaacgctac caataaaata gataaataca ataaacccta tcaacacccc aaacgttaca
      301 actctccggt ttacaataga agtaatggca cgaacatagc gtcctgataa tttatcgtaa
      361 cttgaattga accatcggaa aaaccttcgg agcagttgat tgtttgtttc atgagcatcc
      421 gattcgcgca gcataagcgc acaaagtgca ggagtaaggg taatagccgt aacacccgaa
      481 atcacaatag aaattgccat ggtgattgaa aactgccggt aaaaaactcc aaccggtccg
      541 gataaaaatg caaccggaac gaacacagca gacatcacaa gcgtaatagc gagaatcgcg
      601 ctacctatat cctgcatagc ttcttttgta gctgcaattg gtttaagatg tttttcatgc
      661 attttagcat gcacggcttc tacaacaaca atggcattat ctacaacaat acctatcgcc
      721 aaaaccaagg caaatagtgt caaaagattt agagaaaatc caaagagctg cataaaagca
      781 aacgttccga taagagaaac cggaacagca agaattgaaa ttattgtaag ttttaaattc
      841 tgaagaaaaa taaacaccac aagagcaaca agtataaaag cctctatcag tgttttgatt
      901 acttcatgta tagaagcatc aagaaagcgg gatacatcat aagagagcgt ataagacatc
      961 ccggggggaa attgcgtttc cttaagaaag atcagcctgt ctttaatatt tgcaattact
     1021 tcactcgcgt tcgaaccggg tcgttgcttc aacattattg cagcagaagg tcttccattc
     1081 tctttagaaa gcacgctata cgtttgtgca ccaaattcta tatcggcaat atcttttaag
     1141 cgaagtaatt gtccgtcggc atcaacccgc aacacaacat tttcgtattg cgactgttcg
     1201 gtcattttac cagtgtattt taacacatac tgcatggatt gcgcagtttt tccggacccc
     1261 tctccgactt tcccgggagc agcttcgaga ttcatttcac gtagtcgttc cactacatca
     1321 tctgcagaaa ttgcatatgc ctccattcgt tccggatgca accaaacacg cattgcatac
     1381 tctttcgaac caaggatctg agcaaaacct acaccatcaa tacgctttaa ttccggtaat
     1441 acattgatat ctgcaaaatt aaaaatgaag ctttcgtcca aagttgtatc actgctaatc
     1501 aagttaatga aaaggagcat actgtttacc tctttttctg tagaaacccc tgcacgaatc
     1561 acttcttccg gtagttcatc caatacggtt gcaaccctgt tctgaacgtt caccgctgca
     1621 aaatccggat ctgttccaac ttcaaaaaat acagtgataa tacttgtacc gtcattaccc
     1681 gagacagaac tcatataggt cattcctgga actccattga tagcgcgttc caaaggtgtt
     1741 acaacagcct ttgtacaaac ttctgcattt gctcctgtat aacgcgttgt tacagttaca
     1801 gcaggaggtg caatatccgg atactgagta acaggtaatc ctacgagtgc cagaagccca
     1861 agcagaacga aaaaaattga aattaccagt gaaagtaccg ggcgttcgat gaatttattg
     1921 tacatggtat tccaattaat ttttcgataa atccttcaag atatcacgca tggaaacaaa
     1981 ctgcccgttg atttttgtat cctcacgcaa aagctgcgct ccttcgtaaa cgattttgtc
     2041 tttggtatcc agaacttttt cgataatata aaaatcagga atctcctttt caacagttat
     2101 cactcttgat tttaccaatc cttcttcgtt taatacatat acatattttt tatcctgaat
     2161 ttcaaatact gacttctgcg gaatcagcat cacacctgag aaactactga acaattgaat
     2221 ttttccggta gccccgtgac gcagcaagcg atcaggattt ggaaaccttg cgcgaaatgt
     2281 aattgttccg gttccggttg agacttcacc agctgtggtt tcaataaccc cagtatgcgg
     2341 aaagagttcg ccatttgcca tcacaaaacg gacagatttg cttgtatcgc tatttgactg
     2401 aaggtaattg aggtattcac gctccgtgaa gctgaaataa acaaacactt gatcaagatt
     2461 agaaatagtt gtaagcattg caccttcttc tacaagactg cctaatttaa aaggtattct
     2521 gctaatgata cctgaaaatg gggctcgaat ctctgtaaaa gaaaggatcg ttcgtgcatg
     2581 tgtttcatta gcttgagctt cttcaacttt tgcctgagca gattcataac gaattttgga
     2641 aagctcgagt tcagttgggg aaacaacatt tttttctacc agctgtttgg tgttcttata
     2701 gtcaatttca gcagtttttg cttcgagaat acatgatttc aatatcgcag aagccttggc
     2761 taattcctca cgctgaatgg aattatcgat actgaacaat aattgacctt gagaaaccat
     2821 cttcccttca tcaatgtgat tggctgaaat atacccgttt actctcgacc tgatctctac
     2881 attctgaatt gcagtaacct ctccaacgta cgaggtagcc aatgaagtat ctaatactat
     2941 tggtgatgtt acagaaaaag tttccgtatc cttcacttct tcctggtttg aacaagaaaa
     3001 aagaattgcc gataaaacga aaaattgtaa tgctttcatg gtgtgataaa aatgtaattt
     3061 attccccctg aaatacaggg accggattaa aatgcaggat gcactaatca acaaaaatca
     3121 ggaggagaaa aaagcgagtc aggataacct ttaccttttc taagcgataa gatcgaatgg
     3181 gtagcctgtg ccccttccct ttttacagag ataaacgatt gaccatcctt gagaaataat
     3241 ttcaaatcag atgcatcttc actttctgta aagcaatctg cacatggatc atttccggaa
     3301 ttagtctctt cagtagtaat ttcttctatg aatgcttcgg cgttattaaa atccgaaata
     3361 aatatagaag ataggatata ggcaaactgc acaacaaaca ataattgcct gagtgttgag
     3421 aatattttat accgatttag attattcaaa ccgctgcaaa aataacaaaa gacttaaacg
     3481 taaatgaaaa atgaggtata tgtctctttc aaagcacaac cttatacttc tcaaaaaagg
     3541 cctgccaatc ccaaataaaa taattcatca cctcattcat acgcttgagg aagattggat
     3601 taggagtatt gagtgtgcgg aaataatctg cctggtagtg gttcagattg tatttgtaaa
     3661 agaccccacg ggccatactg tacagaatat tcttggaagg atgattcact ctggaaagga
     3721 agtcgctgta ttccttatag atagcaataa attcatttct atccatgccc atcgaatagg
     3781 cacacttatc aaatatactg tccttaagtg tgtcctccat ttccggatca aaactctcgg
     3841 aaagcaaccg aatattcccc accaaaacga tcatcagaaa ctgttcatca tcagacagtt
     3901 caatgcccgg tgactttgga aagtcaggat cctcattgat tagccctgca acatcgggga
     3961 atgccttttc aacaacctcc agaagattgg agacaaaagc cgtagcgagg gtatcctcgg
     4021 tgatctttct gcgggaaaag cgtgttaaca gtgagatcat tagctttttg aaaggtttga
     4081 ctaaatccta ttcaacaaaa ataaccggac tgagctctgg gttttttaac aggacctggg
     4141 cattttcaac aaagttatta acctactgga aacgctgaat ttaaccccgg ctcctttcat
     4201 cctattcttt aacctccctt caacttatgc acagcactgt aaaaagtcct cctacatttg
     4261 cttcaccaaa acccaaaaga tgaaaacccg acatcttgtc ctggccggat tcctgagtat
     4321 gctggccttc atgacctgga cattaataaa gaacaaagac attttttccc tttccggaga
     4381 aaaaccaaaa gacgcaggta cacgatacgt cgatgttatt tcgagcaagg cagacagtac
     4441 cgaagttctt attgaaggac aaggaagaat ttcatcgagt cggcgaattg atgtatcatc
     4501 cgaagttcag ggattgttat tgcccggaaa aatattaaag cccggcacaa ctgttcgtca
     4561 gggagaacaa ttgtttcagg tacgcgatca ggatgcacga cttgcattac aggcaagaaa
     4621 atcgaattac atgaacatcc ttgcgaacgc attaccggat atccgtcttg atttttcgga
     4681 tgtttacccg gattggaaaa acttttttga tcagcttgat gcagaaaaac cattacccga
     4741 attgcctcaa ccaaaaacag gaagagaaaa gtcgtttctg gcatctaaaa acatattcgg
     4801 tgaatactat actattcgcg cagaagaaga gcgcatacgg aaatacagca tcatcgcacc
     4861 cttcgatgga accataacag atgtttttgc cgaacccgga tctgttgtaa atcccgggac
     4921 acgattatta tccattataa aaggaagtga tcttgaaatt gaattaccaa ttgatgtaaa
     4981 aaatattggc tgggtgaaaa tgggtgcaga agttcaatta aaggcaacat ccggtccggg
     5041 agaatttaaa ggaactgtga tccgcatcgg agagtttgta aatgcgaata ctcaatcggt
     5101 accggtgtat atccgtatca acaaacaaaa cggcgatgcc ctttacaccg gcatgtatct
     5161 tacaggttac atccacgcag gaaaaatccc tggaacaatc agtattcccc gacgcgcttt
     5221 gtttgaagac aatcattgct ttgaagtgaa ggattctgta ttgctcaaac gtcagctaca
     5281 gattgctttt gtaacccggg aatcggtaat agtaagtgga attccaaacg gaacattatt
     5341 ggtggcagaa ccgcttacca atgtttcgga cagtatgcgt gtaactccta ttgttaaaca
     5401 atgagaaggc tcatttcatt tttcataacc aatccgatat ggggaaatgc atttattttc
     5461 ctgaccatcg tatttggttt actcgcattg agcaacatgc ggcgttcctt ttttccggag
     5521 atgcctccgc gtgtcatcac tatcgacgta gcctatccgg gtgcatcacc tgtggagatg
     5581 gaagatggcg ttaccattaa aattgaacag gcactggaag gactcagcgg tattaagaaa
     5641 atcacctcat cgagcgaaga gaaccgtgcc agtttaaaaa ttgaagcctt tgaagataca
     5701 gacatggacg atctgcttac ggatgttgag aacactgtaa acagtattaa ctcgtttccg
     5761 gtaggtgcag aaaaaccaat cattcgcaaa ctaaaggtag gaagcatggg tggaaccgct
     5821 gcctttttat ccctcacagg tccggatgat ttgtggaaac tgaaagcgaa atcggaacaa
     5881 atcaaaaatg attttttagc tgagaaatca atttcacaaa taagagttgt tggttatcct
     5941 gaagttgaaa tttccattga agtaaaagaa agtaaattac tggaatacgg attacgcttt
     6001 gatcagctca cttctgctgt tcgtacaaac aatctggatc tcacgggtgg tacaatcaaa
     6061 acagaagaag aagaatacat catacgtatg cgtgcccgtg agacattggc tgatgaaatc
     6121 ggtaaaattg taatccgcgc aacacccggt ggtgagatta ttcgactgag tgatattgca
     6181 accgttaact atcaatttgc tgatcgtccc tacaaatcct atatcgacgg aaaacgaaat
     6241 atcaacgtaa tcgtatcgaa acttcccgaa gaagatctgg gtgcaattgc caaattcatt
     6301 accgattata ccgataagtt caacgaggaa aatgaggatt ttgaactccg catcattttc
     6361 cagtttgcag atatgttgca ggagcgaatt gatctgctta ccaataatag aattatcggt
     6421 ttctttttgg taatgatttg ccttgggttt ttcctgagtt tgcggctttc attttgggtt
     6481 gcaattggaa ttcccatttc atttctgggt ttgttcattg caggttacct atacggaatg
     6541 accatcaaca tgatctccct atttggaatg atcattgtaa tcggaattct tgttgatgat
     6601 ggaattgtaa ttgcagaaaa tatttttgcg cactacgaac gtggtaaatc acccatgctg
     6661 gctgcacttg acggaactgc agaagtgttt aaatcggtaa ttgcttctgt acttactact
     6721 gttgttgcat tctcactttt aatgtatgtt ggcggtcagt tcgaaatgat gcgcgagatg
     6781 gcatttgctg ttgtggcttg tcttttattt tctcttttcg aagctattct tacactcccg
     6841 caacaccttg gtcacgacag catgttaaaa ccagtgaagt ggggttttta ccagaagatc
     6901 cgaagcagaa ttaacaaatg gatcgatgca ttgcgggatg ggtatgcatg gttgctggga
     6961 agaataatga gtaaacaccg tgctgcaatt tttattccgg tcatttttat ctcaactatt
     7021 attttcctct ttaaatcaga gatcatcaag ttcacctttt tccccaacat tcagtttgat
     7081 gacattactg tttcagttgc ctttaaaccc ggagaacggg aagatcagac ggaagaattc
     7141 cttgaatact gtcaggaaaa agtttatgaa gtccgcgatt cgttggaaaa acactacgga
     7201 gaaccgatca ttactgccac taccctatcc gttggtatgg ccgaattcct tgatgaaacc
     7261 ggggcacacg caggtaatct tcgaatacag attgacattg aaggaaaaga cattgaaagt
     7321 tttaccattg cagaattgat ccgcaaaaag attggtccga ttaccagccc tgaaaaattc
     7381 accattgggg gcgaaggccg gtttggaaaa cctgtcgcgt taatgttgtc ttccaaaaat
     7441 caggatgagc tgctcaaagc gaaagaattc gttaaagata aattcatcaa actgggtcca
     7501 cttaaagatg taaacgacaa cagtccgctc ggctctcagg aaattcatat cgatttattg
     7561 ccgaaagcgt atttgcttgg attaagtcgt tctgaaattg cacgacaaat tcgtcaggga
     7621 ttctttggtg aagaagcgca acgattaata attggagaag atgaagtgcg tgtttgggtt
     7681 cgttatccaa gagaagatcg tcagtatctc ggtcagatcg aagaaatgcg gattaaaaca
     7741 cttaccggag aaacctaccc attaagggag cttgtaaatt tctcgataca aaggggtgaa
     7801 gttggtattc gtcactacaa cggtaaaaaa gaaattactg ttgaagccga tcttgtaaat
     7861 gcaaatgcac cggtaggtga tgcgattgaa ctcgtaacat caaaagttat tcaaccactc
     7921 aatgcacaat ttcccaatgt tgaaacaaac ttcctgggac aggctgaaag agcacgagaa
     7981 acgcagggac cattaatgac agttttggtt ttaaccgttt tgcttgtaat tctaattctc
     8041 gctctgaatt ttggttctgc tatgcaggct ttcttagtac ttacagcaat acctgcagga
     8101 attttttgtg catttcttgg tcacggtctg gaaggaaaac ctgtctctct atttagtttc
     8161 tggggaatca ttgcactgat tggaattctg gtgaatgatg cggtggtgat ggtggatact
     8221 tacaaccgtt atatcaaaaa tggaatgagt gtgaaagatg ccgcctacga agccggaaga
     8281 tcacgtttcc gagcagtatt gcttacatcg cttaccactg ttgttggctt gtatccgtta
     8341 attctggaag atagtttcca ggcgcaattt cttattccga tggcgatttc tgttgcacac
     8401 ggattgttgt ggggaacaat gcttaccatt ctcttcttcc ccagcgtgat tatattctgg
     8461 aatgatgtcc gtcgtatttt atattggttc aagggaaata ccccactcgt ttttgcaatg
     8521 cttttcctta ttattacgct cgccctgaat attgcaattg tattctcacc tcatcttaca
     8581 ggatcagtaa aaatgtcggc aggaataata atgggtgcca taataattca aacactaatt
     8641 ctgcttccct tctttatttg gaagccggaa actgctcacc gcctgctggc tattcatgct
     8701 gctattattt taaatcttgt gctcattgca ctgccaacgg gttatcatat tcttttcatt
     8761 tttccggttg gtgctttcct ctatctggga tatgaaaaaa ccttacgctg gatttttaaa
     8821 tttcattccg acactttccc aaaagcagaa agtgttgagc ctgtcctgcg caaaaacaaa
     8881 cttgaaactc aacttgcaaa tgatatcgtc aatgcaaagg atatttaagc tgtcgtttat
     8941 ttttctgccg ctattcgcta cagcgcagca gaaattgtcg ttatcggacg cgatccgtat
     9001 tgcccttgaa aaaaactaca aggtagaaat tgcgcgcgac cagatttcca ttgccaaaaa
     9061 caacaataac tggggagaag ccggaaggtg gccatcggtc gatctgcaga tcacacaaaa
     9121 caacaacata ctcgaccaaa gcaataaccc tactgctttt atccgcgata agatcattac
     9181 caattctgtg aacggaacac tttctgccaa ctggatgttg tttggcgggt ttcgggttca
     9241 gttcacgaaa gaacgtttgg aacaactgca ggctcaaagc gaaggaaatg ccattcttgt
     9301 agtagaaaac accgtacaaa gtgtaattct cggttattac aatgctgtgc tgcaaaaaga
     9361 aaagctggat gtgctcagaa aactcattaa actttcgcgc gagaagtacg aacacatgga
     9421 acgcaaaaag gatttgggtg taagcggaac tttcgatctg ctccaaatta aaaacgcata
     9481 cctcagcgat tccacttctt ttctccttca ggaactggcc taccgtaatt ccatacgcaa
     9541 cctcaacctg ttgatgaacc aggatgtaga aacattttac gatctcaccg actttctgga
     9601 agtaagaacc gaccgttacg attttgacac gctcaaacga agaatgttgc gtaacaacac
     9661 cactattcta aatcagtaca tcaatctcga attgcaacgc acgcaaaccg atcttgcaag
     9721 aagcgcattg tatccggtag tcagtttaca aatggggacc gctaattcat tcagccattt
     9781 ccaaacatcg atgttcgaat cgaatggttc aaatttgaat tactatgcca attttgttgt
     9841 gaacttcaat ctgttcaacg gtggaaaaac ccgtcgtgcc atcgaaaact ccagaatcca
     9901 ggaaaaaatt caacagacct cactcgacga acaagttttt acattgacaa gtcagctaag
     9961 aaatgcctac gaaacctacg tggcccgaca ggtgattatg aatctgagca ctgagagtta
    10021 tcgctctgct gaattcaatt tacgacttgc tcaggaacgt tttgatcagg gtaccatttc
    10081 atccttcaat taccgggatg tacagttaca gtttttcaat gctgccatgc aaagactgga
    10141 tgccgccaat accctattgg gatctcatac tgagttgtta cgccttaccg gaggaatcat
    10201 cgatctgaat ccgaattaag tatttcactt cccgctgaat tc
//
LOCUS       DAOMYG010000045        10241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2183_length_10241_cov_10.726095, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000045 DAOMYG010000000
VERSION     DAOMYG010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10241
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2183_length_10241_cov_10.726095"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PK610_02505"
     CDS             <1..392
                     /locus_tag="PK610_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRJ34824.1"
                     /translation="ISKQLKKGMSQFLPFAITIVAILLSDLLIGILIGLVAGIYFVIK
                     ANFKSAIHLFRSGNNVLIKLNKDVSFLNKPEIRKKLNEVNDGELLLIDGTNATFIDRD
                     VQEEILTFMEKAQNKNIKVELKNIIIQ"
     gene            389..1027
                     /gene="can"
                     /locus_tag="PK610_02510"
     CDS             389..1027
                     /gene="can"
                     /locus_tag="PK610_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013069836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HRJ34825.1"
                     /translation="MKSYEKLLLENKAWAGIKTASDPSYFPRMARDQKPEFLWIGCSD
                     SRVPANEITNTEPGEMFVHRNIANLVVHTDLNLLSVLQYAVEVLEVKHIIVCGHYGCG
                     GVRAAMTQNHLGLINKWLRNIKDVYRANRSEMEAIKDENKKVDRLCELNVIEQVNHLA
                     ETSIIQRAWKNHKRPMLHGWIYGLNDGIIKEITVVEPNAPIDPLYTFKFDNE"
     gene            1031..1699
                     /locus_tag="PK610_02515"
     CDS             1031..1699
                     /locus_tag="PK610_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRJ34826.1"
                     /translation="MTITLQVEVCNKLFLRDPLETELGKRIIKESIVLLEEIGFEKFT
                     FRKLAEHIESGEPSIYRYFENKHKLLVYLFNWYWAWVECRIQFSIHNISNPKAQLSLA
                     IEQLTQSDQEDLTIAHVDESRLHRIVIRESAKVFQTMDVDQENTEGFFGNYKSLCKTL
                     TEIIQLNRPDYPYAASLSTTLIESAHQQQFLSEHLKSLTSVRKSASSLKEWLEHLAFQ
                     CLEC"
     gene            1739..2692
                     /locus_tag="PK610_02520"
     CDS             1739..2692
                     /locus_tag="PK610_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRJ34827.1"
                     /translation="MAASHRNHAREVFRIEAKAVADLSALLDASFDLAIETILRNKGR
                     LVISGVGKSGIIARKIAATMASTGTPSLFMHPVEAFHGDLGMLKKEDVFLAISYSGET
                     EELLRIVPFIRDRKIALLSITGNPKSTLARNSTAHLNVKVHKEACPLELAPTSSTTAT
                     LAMGDAMAVALMKARKFRSEDFAQFHPGGSLGRKLLTTVEQVMFREHLPLVTPTTTLS
                     DVIAIMTKGRLGLAVVQVDKTVKGIITDGDLRRAMDKHKEKLFSKKASDIMTAKPRAI
                     AKNMKLAEAEEMMSKLKIASLLVVEKNKLAGIVQLYSINND"
     gene            2652..3482
                     /gene="kdsA"
                     /locus_tag="PK610_02525"
     CDS             2652..3482
                     /gene="kdsA"
                     /locus_tag="PK610_02525"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRJ34828.1"
                     /translation="MRESYNFTASTMTDLYSRICKGPFLIAGPCVIESEAMVMDLAEQ
                     LSAIANDLGFTYVFKASFDKANRTSVDSYRGGGMEEGLRILEKVKKELGLAVTTDIHE
                     SNQATAIAEVADILQIPAFLCRQTDLLLASATTGRIVNIKKAQFLSGSEMQFPVNKVK
                     STGNEKIMLTERGTMFGYNNLVVDFRNIADMKSFGYPVCMDVTHSTQKPGGAGGKSGG
                     NREYAPLLASAAAAVGANGFFFEVHRDPDKALSDGPNMIPLSEFRALLEKHVLPHLAK
                     "
     gene            3537..3992
                     /locus_tag="PK610_02530"
     CDS             3537..3992
                     /locus_tag="PK610_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013071740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRJ34829.1"
                     /translation="MHTLKTVQRLPLSMQEAWDFFSSPKNLAAITPAEMGFIIRSEIP
                     EKMYSGLFIHYTVKPLMGIPTKWVTEITHVNEPHFFVDEQRQGPYAVWHHQHHFREVE
                     GGVEMTDIIDYRVPLGVLGKLANDLFVRKKVESIFAYRRKKLIELFGEC"
     gene            complement(4008..5618)
                     /locus_tag="PK610_02535"
     CDS             complement(4008..5618)
                     /locus_tag="PK610_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRJ34830.1"
                     /translation="MKLCIFYILLLLFVLQSCAQMAVPTGGEKDRTPPALVEEQCVPR
                     NGSTMFNGKKIRLEFNEYIKLNNPQQQVLISPSLNGTVKYVQKGKTLELELPGELLPN
                     TTYTLFFGQAVADITEGNIAAELQFVFSTGPILDSLMVTGKVQNAFTSGPEKGVLVGL
                     YADDDSAFYNQKPRYLAITDAAGVFRIKNVAGGTFRLYALRDLNGNLMYDLPGEEAAF
                     FPGTIDPAYLKEELVLPLIKEQKEKWFLRKREFNEPGRGMLVLSRIPEKIPQLYSGKN
                     EGEFSAVENIRLSNDTLLYWITNPEHNELRVEIKDANDELLLSDTIRLSRDAKRRERE
                     LALPLKPEMSFAGNADRFSKMSFSLRDPIVSQQEVSLPFFTDSLSSVLAFTRADERRY
                     ISAHSFLRAAQENYMLIFPEGSISDVFGRKNVQDTVRFKVHKEEFYGKLLLQLSPGSF
                     SGLILQLVNDRNMVLSEKSISTAAAIEFPNLRPGNYRLRIIVDSNSDGKWSMGEMFAR
                     IPPETVLVYPSIITIRSNWDFEVEWKLD"
     gene            complement(5618..6103)
                     /locus_tag="PK610_02540"
     CDS             complement(5618..6103)
                     /locus_tag="PK610_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRJ34831.1"
                     /translation="MAEELHIDTTAGKEAIYQSLLPQLTALVEGEDDLIANLSNLMAA
                     LHSAFGFFWVGVYRVSGNELVLGPFQGSIACTRIAKGKGVCGACWEKEVSIIVPDVDA
                     FPGHIACSSLSRSEIVIPVKNAKNELVMILDVDSDRLNDFDETDRIYLEQVAALIGKM
                     C"
     gene            complement(6182..6784)
                     /locus_tag="PK610_02545"
     CDS             complement(6182..6784)
                     /locus_tag="PK610_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34832.1"
                     /translation="MFRRLFLLLVVCGTIQTARAQYFGFGQLFIQMIFSWEEGGYEAK
                     HTWFNDEPAYLHGLRGGWYVNQDNTLLLGFSMYAGSTTINEQFSLYSRQRFTTFYSTL
                     FMSKTYRADQPIQIEVLGHVGYGFAQLEALGFGAPPTSYSGYLLLEPNVNASFALGNI
                     CRIGAGIGYRQLGKIRLNGADTYSLSGPSLNAFIKFGRFR"
     gene            6836..9640
                     /gene="polA"
                     /locus_tag="PK610_02550"
     CDS             6836..9640
                     /gene="polA"
                     /locus_tag="PK610_02550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRJ34833.1"
                     /translation="MSENDKKLFLLDAFALIFRGYFALAANAKFNPVNSKGVDTSAVL
                     GFTNTLVELLKKEKPTHIAVVFDTAAPTVRHEEFAAYKANRDETPDAIVVGVPYIKEI
                     IKAFRIPVLETDGYEADDVIGTLAKKAEEKGFITYMMTPDKDFGQLVNENILIYKPGR
                     FGNEAEVVGVREVCEKFGIERTTQVIDVLGLMGDKVDNIPGVPGVGPKTAAQLIKDYG
                     SVENVLANTDKLKGKLKENLETYREQALLSKKLATILLDSPVEFNENDLILEEPDKDK
                     LLELFSELEFRTLSKRILNTEIKTPARNGDQIDLFSEGAATNTETEITEGETRQHKTI
                     KDITHHYHAADTPEKVDQLIKELSALSEFCFDSETTSLEPLDAEIVGLSFAWKKQEAW
                     YVPFPTDPNAGKSLLEKFRTVFENENIRKIGQNIKYDYMVLLVQGLEVKGELFDTMLA
                     HYLLEPEQKHGMDELAENYLDYTPVSITELIGKKGKEQGSMRDVPLELITEYAAEDAD
                     ITLQLKTVFEPKLKEREAENLFHGIECPLLKVLAHMELEGVNLDGAFLNTYSTQLGKE
                     MEDLEKEISKAAGTAFNLNSPKQLGQILFEVLKIDDKAKKTKTGQYATSEDVLQKLAD
                     KHPIINMILDFRELGKLKSTYVDALPEMVHPSTGRIHTSFMQAVTATGRLSSNQPNLQ
                     NIPIRTERGRKIRKAFIPRDKNHVLVSADYSQVELRLMAALSGDEHMTEAFRNNLDIH
                     TATAARVFGVPQEEVTKDMRSRAKAVNFGIIYGQSAYGLSQSLSIPQKEAKEIIDQYF
                     TTYPGVKKYMEDCIRKAQEIGFVETLLGRRRYLPNIKASNNTDRGFAERNAINTPLQG
                     SAADIIKIAMINIHNEMRRRNMKSKMVLQVHDELVFDAHISEVAELMPMVKEKMEKAV
                     ELSVPLIVEIQQGKDWLEAH"
     gene            complement(9695..>10241)
                     /locus_tag="PK610_02555"
     CDS             complement(9695..>10241)
                     /locus_tag="PK610_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ34834.1"
                     /translation="GMLTNFNLSSGNTTSNWVDDHAVLSSQICTYYPTVVTVNGGTLT
                     ASGAGVNFQWLECTSGGLVVIPNETNQSFTPTQSGVYSVGLNVGSCMVNSECVSVTVG
                     PTGVDDLISGAISVFPNPVNDQLQISSNERVPFSVFNSIGSMVYNGILTPNATLDVSL
                     YTAGIYYIRTLNGAVVRFVKQ"
BASE COUNT         3105 a         2366 c         2222 g         2548 t
ORIGIN      
        1 ttatcagcaa gcaattaaaa aagggaatgt cgcaattcct tccatttgcc attaccattg
       61 ttgcgatttt attgagcgat ttgctgattg gtattctgat cggactggta gcaggtattt
      121 attttgtgat caaagccaat tttaaatcag ccattcatct gtttcgttcc ggaaacaatg
      181 tgctgatcaa attaaacaaa gacgtttcct ttttgaacaa accggagatc agaaaaaaac
      241 tgaatgaggt aaacgatggt gagctgctgc ttatcgacgg aaccaatgct acgtttattg
      301 atcgcgatgt gcaggaagag attctcacct tcatggagaa ggcgcagaac aaaaacataa
      361 aagttgaatt aaaaaatatc atcatccaat gaaatcctac gaaaaacttc ttctcgaaaa
      421 caaagcctgg gctggtataa aaacggcaag cgatccctct tatttcccca ggatggcgcg
      481 cgaccagaaa ccggaatttc tttggatagg atgttcagac agtcgtgttc ctgccaatga
      541 aattaccaat accgaacccg gcgaaatgtt tgtacaccgc aacatagcca acctggtagt
      601 gcataccgat ctcaatctgt taagtgtatt gcaatatgcc gttgaagtgc ttgaagtaaa
      661 acacatcatc gtttgtggtc attacggctg cggaggtgtt cgtgcggcaa tgacacaaaa
      721 tcatcttggt ttgatcaaca aatggctgcg caacatcaaa gatgtatacc gtgcaaaccg
      781 ttcggaaatg gaagcgataa aagacgaaaa caaaaaagtt gatcgtttgt gcgaactgaa
      841 tgtaattgag caggtcaatc accttgcaga aacatccatc attcagcgcg catggaaaaa
      901 tcataaacgt ccgatgctgc acggttggat ttacggattg aacgatggga tcattaaaga
      961 aattactgtt gtagaaccca atgctcccat agacccgctt tacacgttta aattcgataa
     1021 cgaataagca atgaccatta ccctgcaggt agaggtttgc aacaaactct ttttgcgtga
     1081 tccgctcgaa accgaattgg gcaagcgcat catcaaggag agtattgttt tgctggaaga
     1141 aattggtttc gaaaaattca cttttcggaa actggccgaa catattgaat cgggcgaacc
     1201 ttccatttac cgctatttcg aaaacaaaca caagctgctg gtctacctct tcaactggta
     1261 ttgggcctgg gtggaatgcc gcattcagtt ttccattcac aatatttcca atccgaaagc
     1321 acaattgtcg ctcgccattg agcaactcac ccaatcggat caggaagact taaccattgc
     1381 tcacgtggat gaaagtcgtc tgcaccgcat tgtaatccgc gaatcggcca aggtgtttca
     1441 aaccatggat gtggatcagg aaaacaccga aggatttttc gggaattaca aatccctgtg
     1501 caaaacactt acggagatca ttcagcttaa tcgtccggac tatccctacg ccgcaagttt
     1561 gagcactacg cttatcgaat cggctcacca gcagcaattc ctctccgaac acctcaaaag
     1621 cctcaccagc gtccgaaaaa gcgcgtcctc actgaaagaa tggcttgagc accttgcttt
     1681 tcagtgttta gagtgctgat ctgatctgcc ttgcttatct ttggcggaat tcttgtctat
     1741 ggccgcatca caccgtaatc atgcccgcga ggtttttcgc attgaagcca aagctgttgc
     1801 cgatctttct gcattgctgg atgcttcttt cgatctcgcc atcgaaacca ttctgcgcaa
     1861 caaaggccgg ttggtgattt cgggtgtagg gaagtcagga atcatcgcac gtaagattgc
     1921 cgctaccatg gcaagcaccg gaacgccttc gctgtttatg catcctgttg aggcctttca
     1981 cggcgatttg ggtatgctca aaaaagaaga tgtgtttctg gcgatctcct actccggaga
     2041 aacagaagaa ttattgcgca tcgttccctt tatccgcgat cgcaaaattg cattgctttc
     2101 catcaccggt aatccaaaaa gcacattggc acgcaactct accgctcacc tcaacgtgaa
     2161 agtgcacaag gaagcttgtc cgctcgaact ggcacccacc tcttccacca ctgccacgct
     2221 tgcaatggga gacgctatgg ctgttgcgct gatgaaagca agaaaattcc gctccgaaga
     2281 ttttgcacaa ttccaccccg gcgggagtct gggcagaaaa ctattgacca ctgtagaaca
     2341 agtgatgttt cgtgagcatt taccactggt tacaccaaca actaccttga gcgatgttat
     2401 tgccattatg accaaaggtc ggcttggact ggctgttgtg caggtggata aaacggtaaa
     2461 aggaattatt accgatggcg atctgcgcag agcgatggac aaacacaagg aaaaattatt
     2521 cagcaaaaaa gcgagcgata tcatgactgc taaacctaga gcaattgcca aaaacatgaa
     2581 gcttgccgaa gcagaagaaa tgatgagcaa actgaaaatc gcatccctgt tagttgtcga
     2641 aaaaaacaag cttgcgggaa tcgtacaact ttacagcatc aacaatgact gatctctatt
     2701 cccgtatttg caaaggaccc ttcctcattg ccggtccatg cgtaattgaa agcgaagcca
     2761 tggtaatgga tctcgccgaa cagctgagtg ccattgcaaa cgatcttgga tttacctatg
     2821 tgttcaaagc ctcttttgat aaagccaatc gcaccagtgt tgattcttac cgcggagggg
     2881 gaatggaaga aggattgcgc atccttgaaa aagtaaaaaa agaacttggt cttgccgtta
     2941 ccaccgatat ccacgagagt aatcaggcta ctgccattgc agaggtcgct gacattttgc
     3001 agatccctgc atttttgtgc cggcaaacag atctattatt ggcttctgca acaacaggca
     3061 ggattgtgaa cattaaaaaa gcacaatttc tttcgggatc agaaatgcaa ttcccggtga
     3121 acaaagtaaa aagtacgggt aacgaaaaaa taatgctaac ggagcgcgga accatgtttg
     3181 gctacaacaa tctggtagtg gattttcgca atattgccga catgaaatcc tttggttacc
     3241 cggtgtgtat ggatgtcacc cacagcacac aaaaacccgg cggcgcaggt ggtaaaagcg
     3301 gtggtaatcg ggagtacgca cctttacttg catcggcagc agcagcggtt ggtgccaacg
     3361 gattcttttt tgaagtacac cgcgatccgg acaaagccct ttctgatggt ccgaacatga
     3421 ttcccctgag cgaattccgc gctctgctgg aaaaacatgt gcttccgcat ttagcgaaat
     3481 aaccctcatt gcttgtcgtg tactgaacaa tttcccttat tggaagttat tcacctatgc
     3541 atacactcaa aaccgttcag cgcctcccgc tttcgatgca ggaagcatgg gattttttct
     3601 cttccccaaa aaatctcgct gcaataacac ccgccgaaat gggatttatc attcgttctg
     3661 aaatcccgga aaaaatgtat tcgggattgt tcattcatta cactgtaaaa ccactaatgg
     3721 gcattccaac aaaatgggtt acggaaatta cgcatgtaaa cgaaccgcat ttttttgtcg
     3781 atgaacaacg tcagggccct tatgccgttt ggcaccacca acatcatttc cgcgaagtag
     3841 aaggcggagt agaaatgacc gacatcatcg attaccgcgt tccattagga gtgctcggta
     3901 agcttgccaa tgatttgttt gtccgtaaaa aagttgaaag catttttgcc taccgcagaa
     3961 aaaaactgat tgagttattc ggcgagtgct gatggaattc ggactactca atccaatttc
     4021 cactccactt caaaatccca attggagcga attgtaatta tcgatggata caccaatacc
     4081 gtttccggcg gaatacgtgc aaacatttct cccatcgacc atttgccatc gctgttgcta
     4141 tccacaatga tccgcaatcg gtaattgccc ggacgaaggt tcggaaattc aattgcagct
     4201 gcagtgctta tgcttttttc agataacacc atgttgcggt cgttcaccaa ttgtaaaata
     4261 agaccggaaa aactccccgg agacaattgc aaaaggagtt ttccgtaaaa ctcttcctta
     4321 tgcactttaa agcgaacggt atcctgtaca ttttttcttc caaacacatc gctaatactt
     4381 ccctccggaa aaatgagcat ataattctcc tgagctgcac gcaaaaagga atgcgcacta
     4441 atatacctac gctcatcggc acgtgtaaat gcaagcactg aggacagtga atcagtaaaa
     4501 aaaggaagag aaacttcttg ttgtgaaaca atcggatcgc gtaatgaaaa actcattttg
     4561 gagaagcggt cggcattacc tgcaaaactc atttcaggtt ttagcggcaa tgccaattcc
     4621 cgttcgcgtc gttttgcatc gcgtgacagg cgaatggtat cgcttaacaa aagctcatca
     4681 ttggcatctt taatttccac tcgaagctca ttgtgttcag gattggtgat ccaatacaac
     4741 aaggtgtcgt tactcaaccg aatattttct actgcagaaa actccccttc gttttttccg
     4801 gaatacaatt gcggaatttt ttcggggata cggctaagca cgagcattcc tctgcccggt
     4861 tcgttaaatt cgcgtttgcg taaaaaccat ttttcttttt gttctttgat caacggcaaa
     4921 accaattctt ccttcaggta tgcaggatca atagtgcccg gaaaaaacgc tgcttcctct
     4981 ccgggtaaat cgtacatcag atttccattg agatcgcgca aggcatacaa tcgaaaggtt
     5041 ccgccggcaa cattttttat tctgaacaca cctgctgcat cggtaatggc caaatacctt
     5101 ggtttctgat tgtaaaaagc agaatcatcg tcggcataca atccaactaa cactcccttt
     5161 tccggacctg aagtaaaggc attttgaact ttacctgtaa ccatcagcga atcgagaatc
     5221 ggtccggtag aaaatacaaa ctgcaattct gctgcgatgt ttccttcggt gatatcagca
     5281 accgcctgtc caaaaaacag ggtataagtg gtattcggca aaagctctcc gggcaactcc
     5341 agttcgaggg ttttcccctt ctgcacatat ttcacagttc cgttgagcga aggtgaaata
     5401 agaacctgtt gttgcggatt attcaacttg atgtattcgt taaactcaag gcggattttt
     5461 tttccgttaa acatggtgct tccattccgc ggaacacatt gttcttccac taaggcgggc
     5521 ggcgttctgt ctttttctcc gcctgtagga acagccattt gtgcgcagga ttgaagcaca
     5581 aacaaaagca ggaggatata gaagatacac aatttcatca gcacatcttt ccgattaatg
     5641 cagcaacttg ttcaagataa attctatccg tttcatcaaa atcatttaaa cggtcgctgt
     5701 ccacatcgag tatcattacc aactcatttt ttgcattttt aacaggaatc acaatctccg
     5761 aacgcgaaag cgagctgcat gctatatgcc cgggaaaggc atccacatcg ggaacaatga
     5821 tgctgacctc tttctcccag caagcaccgc aaacaccctt tcctttcgca atacgtgtgc
     5881 aggcaatact accttggaat ggcccgagta caagttcatt gccgcttaca cgatacactc
     5941 ccacccagaa aaaaccgaat gccgaatgca atgccgccat cagattggaa agatttgcaa
     6001 taaggtcgtc ttctccctct accaatgcag tgagttgcgg gagtaaggat tggtaaatag
     6061 cttctttacc tgcagttgta tcgatatgta actcttcagc cataatcagc acgaaggtcg
     6121 caaaatcagc ggatcaattg caatggaagc aacaaacgcg ttaaggaaaa aagaaaaaca
     6181 attatcggaa tcggccaaac ttgataaagg cattaagtga gggtccgctg agcgaatagg
     6241 tatctgcacc attgaggcga atcttgccca gctgacgata gccaatgcct gcaccgatac
     6301 ggcaaatgtt tccaagtgca aaggaggcat ttacattggg ctcaagcaac agatatcccg
     6361 aataggaagt tggaggagca ccaaagccca gcgcttccag ttgtgcaaat ccgtagccta
     6421 catgacccag cacctctatc tgaataggtt gatcggcgcg ataggttttg ctcataaaca
     6481 aggtggaata aaaggtggta aaccgttgcc tgctgtaaag cgaaaactgc tcgttgatgg
     6541 ttgttgatcc tgcatacatg gaaaacccaa gcagcagggt gttgtcctga ttaacgtacc
     6601 atccgccacg caatccgtgc agataagcag gctcatcatt gaaccaggta tgtttcgctt
     6661 cgtaaccgcc ttcttcccag gaaaaaatca tttgaatgaa gagctgacca aagccaaaat
     6721 actgcgcacg tgcagtctgg atggtgccac aaacaaccag caaaaggaac agccttcgga
     6781 acatgaaagc aatttatgaa aaaactctcc ttatcttgcc tctgaaactg aaatcatgtc
     6841 tgaaaacgat aaaaaactct ttctgctgga tgcctttgcg ctcattttcc ggggctattt
     6901 tgcgctggca gcgaatgcta aattcaatcc ggtaaactca aagggggttg atacctccgc
     6961 tgtattggga tttaccaaca cactggttga actgctcaaa aaagaaaaac caacacacat
     7021 cgccgttgta ttcgataccg cagccccgac tgtgcgtcac gaagaattcg ccgcctacaa
     7081 agccaaccgc gacgaaaccc ccgacgctat cgtggtgggt gtgccttaca tcaaggaaat
     7141 cataaaagca tttcgaattc ccgttttgga aaccgacgga tatgaagcgg acgatgtaat
     7201 tggtacactg gcaaaaaaag cggaagaaaa gggattcatt acctacatga tgacccccga
     7261 taaagatttt ggtcagctcg taaacgaaaa tattctcatt tacaaacccg gtcgtttcgg
     7321 caatgaagca gaggtggttg gagtccgtga ggtatgtgaa aaatttggta ttgaacgcac
     7381 cacacaagtg atcgatgtac ttgggcttat gggtgataag gtcgataaca ttcctggtgt
     7441 gcccggtgtt ggaccaaaaa ccgctgcgca actcatcaag gattatggca gtgtagagaa
     7501 cgttcttgcc aataccgaca aactcaaagg caaactgaaa gaaaatcttg aaacgtatcg
     7561 cgaacaagct ttgctttcca aaaaactggc caccatttta ctcgattctc ctgttgagtt
     7621 taatgagaat gatcttattc tggaagaacc cgacaaagac aaactgcttg aactgttcag
     7681 cgagttggaa ttccgcacac tcagcaaacg cattctcaac accgaaataa aaacaccggc
     7741 acgcaatggc gatcagatcg acctcttcag cgaaggagca gcaaccaaca ctgaaacaga
     7801 aattaccgaa ggcgaaacgc gtcaacacaa aaccataaaa gatatcacgc atcactacca
     7861 tgcagccgat actcccgaaa aagtggatca actcattaag gaactttcag cactcagcga
     7921 attttgtttc gatagtgaaa ccacttcact tgaaccgctt gatgcagaaa ttgtgggctt
     7981 gtcttttgca tggaaaaaac aagaagcctg gtatgttcct tttcccaccg atccgaatgc
     8041 aggtaaatcc ttactggaaa aattcagaac ggtattcgaa aacgaaaaca tccgcaagat
     8101 tggacagaac atcaaatacg attacatggt gttgctggta cagggactcg aagtaaaagg
     8161 cgaattgttc gataccatgc ttgctcatta ccttttggaa cccgaacaaa aacacgggat
     8221 ggatgagtta gcggaaaatt acctggatta cactccggtt tccatcaccg aactcattgg
     8281 taaaaaagga aaagaacaag ggagcatgcg cgatgttccg ctggagttga ttaccgaata
     8341 cgcagcagaa gatgcagata ttacactgca attgaaaact gtgtttgaac caaaactgaa
     8401 agaacgcgaa gcagaaaatc tttttcatgg tattgaatgt ccgctgctaa aagtgctggc
     8461 gcatatggag cttgaaggag tgaatctgga tggtgcattc ctcaatacgt attccacgca
     8521 gcttggaaag gaaatggaag atttggaaaa ggaaatcagc aaagccgcag gaaccgcttt
     8581 caatctgaat tcgcccaagc aactcggaca aattcttttt gaggtgctga agatcgatga
     8641 caaagcaaaa aagacaaaaa ccggtcagta cgccacctcc gaagacgttt tgcaaaagct
     8701 tgccgacaaa caccccatca tcaacatgat tctcgatttt cgggaattgg gtaaattgaa
     8761 atcaacctat gtagatgcgc tgccggaaat ggtacatccg tcgaccggaa gaatacacac
     8821 cagttttatg caggcagtaa ctgctaccgg gcgactctct tccaaccagc ctaacctgca
     8881 gaacatcccc atccgaacag agcgcggaag aaagatccgt aaagcattta ttccccgcga
     8941 taagaatcat gtgctggtat ctgccgacta ttcgcaggtt gaactaagat tgatggctgc
     9001 attgagcgga gacgagcaca tgacggaagc ttttcgcaac aatctcgaca tccacaccgc
     9061 cacagcagca cgcgtatttg gcgtaccgca ggaagaagtc accaaagaca tgcgaagccg
     9121 cgccaaggca gtgaattttg gaatcatcta cggacaaagc gcatacggtt tatcgcagtc
     9181 gctctctatt ccccagaaag aagcaaaaga gatcattgat cagtatttca caacctatcc
     9241 gggagtaaaa aaatacatgg aggattgcat ccgcaaagca caggagatag gatttgttga
     9301 aacactactc ggacgcagac ggtatttacc caacatcaaa gcgagcaaca ataccgacag
     9361 aggatttgca gaacgaaacg ccattaacac gcccttgcaa ggttcagcag cggatatcat
     9421 aaaaattgcg atgatcaata ttcacaacga gatgcgtcgc cgcaacatga aaagcaaaat
     9481 ggtgttgcag gtgcacgatg aattggtttt cgatgcgcat ataagtgaag ttgcagaact
     9541 catgccgatg gtgaaggaaa aaatggaaaa agctgtcgag ttatcggtgc cactcattgt
     9601 ggagatccaa caaggtaaag actggctgga agcacattga tgaaataaaa agagagtcca
     9661 tgtggactct ctttttattt tcaaaatgta ccaattattg ttttacaaag cgaacaaccg
     9721 ctccgttcag cgttctgatg taatagattc cagcagtata taaacttaca tctaatgttg
     9781 cattaggggt tagtattccg ttataaacca tcgaacctat agaattaaac acagaaaacg
     9841 gcactctttc attggaggaa atttgaagtt gatcatttac cggattcgga aaaacagaaa
     9901 tagcacccga gatcaaatca tccactcctg ttggaccaac agttacagaa acgcactcgg
     9961 aattcaccat gcagctgcca acatttaatc ctacagaata aacaccggac tgcgtaggtg
    10021 taaacgattg gttggtctca ttgggaataa caacaagtcc gccgcttgta cattccaacc
    10081 actgaaaatt aactcctgct ccactggctg ttaatgtacc tccgtttact gttacaacag
    10141 ttggatagta agtacagatt tgtgaagaga gcacagcatg gtcatctacc caattggatg
    10201 tagtgttgcc ggaagaaaga ttaaaattgg taagcattcc g
//
LOCUS       DAOMYG010000046        10221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2190_length_10221_cov_7.167618, whole genome shotgun sequence.
ACCESSION   DAOMYG010000046 DAOMYG010000000
VERSION     DAOMYG010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10221
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2190_length_10221_cov_7.167618"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /gene="ffh"
                     /locus_tag="PK610_02560"
     CDS             <1..1296
                     /gene="ffh"
                     /locus_tag="PK610_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRJ34835.1"
                     /translation="QGKITEINVAETLKEVRRALLDADVNFKTAKDFTERIKEKALGQ
                     NVLTSVSPGQLLIKITHDELAELMGGQKEEINLAGSPAIILMSGLQGSGKTTFSGKLA
                     NFLKTKRGRKPLLVACDVYRPAAIDQLHVLGEQLGIDVFSDKEEKDPVKIATKAIVFA
                     RSNGFNTVIVDTAGRLAVDEEMMREIEAVKKSIQPSETLFVVDSMTGQDAVNTAKAFN
                     DRINFDGVVLTKLDGDTRGGAALSIRSVVEKPIKFIGTGEKMEALDVFHPDRMADRIL
                     GMGDVVSLVERAQEQYNEEEARKLQKKIAKNQFDFTDFYDQLQQIKKMGNVKDLMGMI
                     PGMGKMVKDVEIGDDAFKHIEAIIQSMTPKERKNPDLLTGSRRKRIAEGSGTNIQEVN
                     KLMKQFEDTRKMMRMMSDKRSMMNMMRQMKNMPGGMPKM"
     gene            1340..2059
                     /locus_tag="PK610_02565"
     CDS             1340..2059
                     /locus_tag="PK610_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34836.1"
                     /translation="MKTILLSLFLIFTVGTTVFAQSNPIQESAREKMLSEKNLRKDFI
                     SGKRWKVILFKQQDETVALKENDFAWFRPGGTYEGALMGLWDSGLWTYEKDEKTITLN
                     LRTGGTKTWKIIESTATSLKLNSGEEELHFIPDLEIKTDVVVTGMKKEISRTWKVVEH
                     SQNNLKLIPKAADFIRFHPDGNYEQCLNNNYKLGSWSLDEKGKTIIITFSKSVIWNIT
                     RLESGVLHLTKKEGNEQIVLK"
     gene            complement(2081..2593)
                     /locus_tag="PK610_02570"
     CDS             complement(2081..2593)
                     /locus_tag="PK610_02570"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HRJ34837.1"
                     /translation="MDRKLIIMPSFDIVSKVDLQTLDNAINAAVREIATRFDFRDSKT
                     SIELDKKNYSVNVLTENEMRMDAVEKILIGRFVKQNLDPKALDFGKDRYASGNMMRKD
                     IKIKQGIDKESSKKIIKLIKDSGLKVQASILDEQVRVTGKKIDDLQEVMALCRQKDVG
                     IPLQFDNMKS"
     gene            2795..3256
                     /gene="bcp"
                     /locus_tag="PK610_02575"
     CDS             2795..3256
                     /gene="bcp"
                     /locus_tag="PK610_02575"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HRJ34838.1"
                     /translation="MNMLKEGDKAPSFTYTNEKGKKVSLKDLKGKVVALFFYPKDLTP
                     GCTAEACNLRDYYSELKKKGIVVIGVSADSEKMHERFREKHQLPYPLIADEEKELIQA
                     YGVWGPKVFMGKKFDGINRTTFIIDEAGKIRNVIAKVKTKEHAEQIFELIK"
     gene            3347..4354
                     /gene="recA"
                     /locus_tag="PK610_02580"
     CDS             3347..4354
                     /gene="recA"
                     /locus_tag="PK610_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRJ34839.1"
                     /translation="MATETTNKEKLKALQMTIEKLEKTFGKGSIMKLGAGAVDQVEVI
                     PTGSLSLDIALGIGGLPKGRVVEIYGPESSGKTTLAIHAIAQVQKQGGIAAIIDAEHA
                     FDQFYAKKLGVDIENLLISQPDNGEQALEIADNLIRSGAIDLIVIDSVAALTPKAEIE
                     GEMGDSNVGMQARLMSKALRKLTGSINKAGCCCIFINQLREKIGVMFGNPETTTGGNA
                     LKFYASIRLDIRRIGQIKDGEQIQGNRARVKVVKNKVAPPFRTAEFDIMYGEGISRVG
                     EIIDLGVEKEVIKKSGSWFSYNETKLGQGRDAVKQLLSDNPELLDELETKIREILNKE
                     N"
     gene            5074..6282
                     /locus_tag="PK610_02585"
     CDS             5074..6282
                     /locus_tag="PK610_02585"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRJ34840.1"
                     /translation="MENFEVPDFEAKYTEGKFRLAWRLCIILASLFTVMGFIEFFHSI
                     ANGILYLICASIPGLELVYLYKTKNSRIAYLSLCSFGSILTAVSLHFFINEVHLGDFI
                     WMALISIMAFWGLGNFWGWFFTVLNFLISLSYFFCSLKTNFEVIRPIDFTLQASLAVE
                     IMAAYIAIAAIVSQFVKYYRYSFESVLSNNLVIKESNRIISSKNEENIILMKEVHHRV
                     KNNLQIITSLLRLQKQTMPEDAQPKFEEAINRIMTMSLIHRKLYQSADFTRIDIGAYI
                     NDLIREIVQSHSVNDNVKAEIKSEYNKIGLNSIVPLGLIINELLSNSFKHAFSNGKSG
                     AINVEISGGPGNTFYLKYSDTGIWIDEDREGNSFGLDLIATLTEQLEGSFTRSGSSYH
                     FTFNNQDSNT"
     gene            6369..7448
                     /locus_tag="PK610_02590"
     CDS             6369..7448
                     /locus_tag="PK610_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ34841.1"
                     /translation="MMINSQYFRNMNSLRLLALLPLLCTLIFFSCNSNETGEVIANKD
                     SIPQNDSLNEQQRLIADLDKRIVSDPDNPNHYHARAFAYFRLAQYSEAMQDIERCIRI
                     DSTIAIFHYTKGEILFAELKVEEALQSYRAALRQNPNYFDAEFRIGRIHMYIKSWNEA
                     MKHINAGLKLNPNFAEGYFMKGEVYEELRDTAKAASSFQTAVEQDPNYYEAYIRLGLL
                     YANAHNKLALDYYNSALSIRPNSVEALYDKAIFMQDHGMIADALSTYDKIIEINPRLE
                     LPWFNKGYIHLVETANYNEALVMFDKAIEINPRYIDAWHNRGLTYEGLKNYPKARENF
                     KKALELDPTYDLSAEAMDRLDRMKK"
     gene            7584..8696
                     /locus_tag="PK610_02595"
     CDS             7584..8696
                     /locus_tag="PK610_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34842.1"
                     /translation="MRYYLIVFFLLQNMISYSQIRVEDFYKKSDGKDDAPSIQRAMRH
                     IDSLGRGSVEFEGSKKYLLQSAVELPRYAVGGRRIIILNGNGCEIVGKSGIDIFRRIP
                     IDQDEALNKMMATRFVFNDFSFTGGSKAINLGATYGSSIQRCNFVTQEEAAIDIQFGL
                     ATEIHHCILTNPKKDGIVLRCGTDWGGSTNNSQSNSSVISMCRAYATKSTDAVFRILG
                     SGGVVLRDCISEGSGDAQYSVFFDRQNSTTVRLFRVENFHLEHAPKKAGIYLNHTGIA
                     TIDGLFAQMAYKDFPLVMSAPNAEQITLINIPHFVGGMVLYSGNNEVPWRLEYCHKSF
                     YAKENWRINTSKGVETKLPFYFSGIGGKFQIKQEYK"
     gene            complement(9066..9833)
                     /locus_tag="PK610_02600"
     CDS             complement(9066..9833)
                     /locus_tag="PK610_02600"
                     /inference="COORDINATES: protein motif:HMM:NF020442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HRJ34843.1"
                     /translation="MPKRKIKIINAKSFLEIIQVLLSDELNCGHVVFRGIADKKNHKL
                     IPSFGRIKEEATGFDLKQYEFETLARFKKRANADVNPKPHNDWEWLALAQHHGLPTRL
                     LDWTSSPLIALYFATKPSLKPDGTIENCNENGGTIYCMHTCNYINTDVEANPFSYEHH
                     GLFYPMHVTKRISGQCGLFSIQPDPSKEFHVGFPFSEANSLIRIQFSKKVSSEIQKKL
                     YLLGIRHESIFPDLDGFTYDLKVKFNLTSCHAIDKLC"
     gene            complement(9838..>10221)
                     /locus_tag="PK610_02605"
     CDS             complement(9838..>10221)
                     /locus_tag="PK610_02605"
                     /inference="COORDINATES: protein motif:HMM:NF037404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34844.1"
                     /translation="GRVKSAILKGEDRPSIEELRGHQKDGIQDLIGWLKGDPKIEFNE
                     EWWNQADNMKNKGFYVGFNKGNAQWESPDKVDEKQYKKSYKIVNEFLDLVYNMEVYAK
                     DPEAIKIYEEMKNKSATDDEKTNLN"
BASE COUNT         3126 a         1944 c         2201 g         2950 t
ORIGIN      
        1 cagggtaaga ttactgagat caacgtagct gaaaccctca aggaggttcg tcgtgcgctg
       61 ttggatgctg acgtaaactt caagacggca aaggatttta cggaacggat caaagagaaa
      121 gctctcggac agaatgtatt aacctcagtt tcacctggac agctgcttat caaaatcaca
      181 cacgatgagt tggcagaact catgggaggg cagaaagagg agatcaatct tgcaggtagt
      241 ccggctatca ttctgatgtc aggattgcag ggttcaggaa aaactacatt ttctggtaag
      301 cttgcaaatt tccttaaaac caaacgcgga cgtaaaccat tgctggtggc ctgcgacgtt
      361 tatcgtcctg ccgctattga ccagttgcat gttttaggtg agcagttggg tattgatgta
      421 ttttcagata aagaagaaaa agatcctgta aaaattgcca ccaaggcgat cgtgtttgca
      481 cgatccaatg gtttcaatac agtgatagtg gatacagcag gtcgactggc ggttgatgaa
      541 gaaatgatgc gcgaaatcga agcggtgaag aaaagcattc agccttcaga aacgctcttt
      601 gttgtagatt caatgaccgg tcaggacgcg gttaatacag ctaaagcgtt taatgatcgc
      661 atcaatttcg atggagttgt ccttacgaaa ctcgatggtg atacccgtgg tggagcagcg
      721 ctttctattc gcagtgttgt agagaaacct atcaagttca ttggtacagg tgaaaaaatg
      781 gaggctttgg atgtcttcca ccccgaccgt atggccgacc gtattttggg tatgggtgac
      841 gttgtttcac ttgttgaacg tgcacaggag caatacaacg aagaagaagc gaggaaatta
      901 cagaagaaaa ttgccaagaa tcagttcgat tttaccgatt tctacgatca actgcagcag
      961 attaaaaaaa tgggtaatgt aaaagacctg atgggaatga tacccggaat gggaaaaatg
     1021 gtaaaagatg tggaaattgg agatgacgca ttcaaacaca tcgaagccat tattcaatcc
     1081 atgacgccga aggaacgcaa gaaccccgat ctacttaccg gttcgaggcg caagcggatt
     1141 gcagaaggtt caggaacaaa cattcaggag gtgaataaac tcatgaagca gtttgaagat
     1201 acccgtaaaa tgatgcgcat gatgagtgat aagcgaagca tgatgaacat gatgcgtcaa
     1261 atgaaaaaca tgcctggtgg aatgccgaaa atgtaattac ggtctgattt ttgatgaatg
     1321 ttactccaaa taattgacaa tgaaaacaat tctcctttcg ctgtttctga ttttcactgt
     1381 gggcacaact gtttttgctc aaagtaatcc tattcaggaa tcagcccgtg agaaaatgtt
     1441 gtctgaaaaa aacctgagaa aggatttcat ctcgggtaaa cgttggaagg ttattctgtt
     1501 caaacaacaa gatgaaacag tagcacttaa ggaaaatgac tttgcatggt ttcgtccggg
     1561 cggtacctat gaaggagcat tgatgggact ttgggattcc ggactatgga cttacgaaaa
     1621 agatgaaaaa accatcacac taaacctgcg caccggtggc acaaaaacct ggaaaataat
     1681 tgagagtaca gctacctctc ttaagttaaa ttccggtgaa gaggagttgc attttattcc
     1741 tgaccttgaa attaaaaccg atgttgtggt gacggggatg aaaaaggaaa tttccagaac
     1801 atggaaagta gtggagcact cgcaaaacaa tctcaaactg attccaaagg ctgctgattt
     1861 cattcgtttt catcctgatg gaaattatga gcaatgcctt aataacaatt acaaactcgg
     1921 aagttggtca ttggatgaaa aagggaaaac cattataatc accttttcca aaagcgtaat
     1981 ctggaacatc acccgtctcg aaagcggcgt tcttcacctc accaaaaaag aggggaacga
     2041 acaaattgtg ctgaaataaa aagtaccgtc agccaaattc tcaggacttc atattgtcga
     2101 actgaagagg aatacctaca tccttctgac ggcataaggc catgacttcc tgcaaatcat
     2161 ctattttttt tccggtcacg cgtacttgtt catccaaaat actggcttga acctttaatc
     2221 cggagtcctt aatcagtttt ataattttct tggatgattc cttgtcaata ccctgtttga
     2281 tcttaatatc ctttcgcatc atatttcccg aagcataacg atccttacca aaatccagcg
     2341 ctttgggatc gaggttttgt ttaacaaagc gtccgatcaa tatcttctca acggcatcca
     2401 ttcgcatttc gttttcggtc agcacattta ccgaataatt ctttttatcc agttcaatgc
     2461 ttgttttcga atcgcggaaa tcgaaacggg ttgcaatttc cctgacggct gcattaattg
     2521 cgttatcgag ggtttgcaaa tcaactttgc ttacaatatc aaatgagggc ataatgatga
     2581 gttttctgtc caaaaatcca cattttttcc ggaaccccga ccgaactgta atattgacaa
     2641 tattattgtc aataatctat ttacctataa ttcagttaat taacatattt tttgcatttt
     2701 tctccctctg actattgctt ttgaactttt tcttcatgtt ctttgtatag cgctgttttg
     2761 acaggaagtc agtcggtgtt ttgacgaaaa agctatcaat atgttaaaag aaggagataa
     2821 ggcaccgtcc tttacttata caaatgaaaa ggggaaaaaa gtctccctga aagatttgaa
     2881 aggaaaggtg gttgccttgt tcttttaccc aaaagatctc acacccggtt gtacagctga
     2941 ggcgtgtaat ctgcgtgact attacagtga acttaaaaag aagggaattg ttgtaattgg
     3001 agtgagtgca gattcggaaa agatgcatga gcggttccgt gaaaagcacc aattacccta
     3061 tcctttgatt gcggatgagg aaaaagagct gatacaggca tatggtgttt ggggaccaaa
     3121 ggtgtttatg ggaaagaaat ttgatgggat aaaccggaca acctttatta tagacgaagc
     3181 aggtaagatc aggaatgtga ttgccaaggt caagaccaaa gaacacgcag aacagatatt
     3241 tgaattaata aaatagaaaa aaaaacagca gcgcagattc actgcgttct gatacttcaa
     3301 ctttgcatta tacaattaca gacccctaac cctaaacaga aaaaacatgg caacagaaac
     3361 taccaacaaa gagaagctta aggcgcttca gatgaccatt gagaaactgg agaagacctt
     3421 tggtaaagga agtataatga aactgggcgc aggcgcagtg gatcaggtgg aagtgattcc
     3481 aacaggatca ttatcacttg atatagcatt aggcattggc ggtcttccaa aaggaagagt
     3541 ggttgaaata tatggtccgg aatcttcggg aaaaaccact ttggccattc atgccattgc
     3601 gcaggtgcaa aagcaaggcg gaattgctgc aattattgat gctgagcatg cattcgatca
     3661 gttctacgct aaaaaactcg gcgtggatat tgagaaccta ttgatttctc aaccggataa
     3721 cggagagcag gcgcttgaga ttgcagataa tctgattcgt tcaggagcaa tcgatcttat
     3781 cgttatcgac tccgtagccg cactaactcc taaagcggag attgaaggtg agatgggtga
     3841 ttccaatgta gggatgcagg cacgtttgat gtcgaaagca ttgcgtaagc ttaccggctc
     3901 tatcaacaag gcaggatgct gctgtatctt catcaaccag ttgcgtgaaa aaattggagt
     3961 gatgttcgga aatcctgaaa ccacaactgg aggtaatgca ctcaaattct atgcttcgat
     4021 tcgacttgat atccgtcgta tcggacagat caaggatggc gagcagattc agggtaaccg
     4081 tgcgagggtg aaagtggtga agaataaagt tgcaccgccg ttccgaaccg cagaatttga
     4141 tattatgtac ggcgaaggaa tttcccgagt tggggagatc atcgatctgg gtgtagagaa
     4201 agaagtcatt aagaaaagcg gaagctggtt tagctacaat gaaaccaaat tgggtcaggg
     4261 tcgcgatgcc gttaagcaat tgttatcaga caaccctgag ttgttggatg agttggaaac
     4321 caagattcgt gagattttga ataaggagaa ctaatacatt tccgggaaac cactaataag
     4381 ccggagtgcg accggaaaaa tggaggacca aaatccttca tcctggtcgc cttacggcgc
     4441 caatggaaag gatggtgtca cttagactcc actcagggac ctggttgtta gtggtgatta
     4501 ggtaacagtg aaaggtgaat agttggttgt aataagtgaa aagtgaatag cgattgctga
     4561 aaagtggata gtccaaatgc agagatcctc attagttcgt ttcacttccg gacaaagaaa
     4621 tagttcattg atcacttcgg cttttcgtaa ttggtaattg attaacaggt aatcggaaat
     4681 tgacaaattg ataattggaa attgaataat tgataattgg aaattaaaaa aaactccggc
     4741 gggcttgcgc tcctgctacc tccaatagct cgtcggaacc atcctgaaca cgggtcattc
     4801 gttgccaatg ccatgatgac agagacacgt gctgagggtt agggttggac aggttctgat
     4861 gctgacctgt catggttgtg cgaaagtcga ttcgactggc tgctgatcaa acaggaaagt
     4921 agactgaact gtggtctgtg acctccttgt aactgtggtt gtatctgcaa ctcagcgaca
     4981 aggaggtttt tttattttgt gctaacaacg atcctgtttt tcgttttgca ataatgctaa
     5041 attgcattcc tgaatattgc cctgaaaaca ataatggaga attttgaagt gcctgacttt
     5101 gaggcaaagt atacagaagg taaatttcgc ctcgcctggc gattgtgtat tattctggct
     5161 tctttgttca ctgttatggg atttattgag tttttccact caattgccaa tggaatcctc
     5221 tatctgattt gtgcatccat tcccggactg gaactggtct atctgtacaa aacaaaaaac
     5281 tcaagaattg cctatttatc gctgtgttct ttcggatcta tacttacagc agtatcactc
     5341 catttcttta ttaatgaagt ccatttgggc gattttatat ggatggccct catctccatt
     5401 atggcgtttt ggggacttgg aaatttctgg ggttggtttt ttacggtgct caattttctg
     5461 atatccctta gctatttctt ctgttcattg aagaccaatt ttgaagtaat tcgtcctata
     5521 gatttcaccc tccaggcttc cctggctgta gaaattatgg ctgcttatat cgcaattgca
     5581 gctattgttt ctcagtttgt aaagtattac cgctattcct tcgaaagtgt gctatctaat
     5641 aacctagtca taaaggaaag taaccgaatc atctcttcca aaaacgaaga aaacatcatt
     5701 ctgatgaagg aagttcacca tcgggtgaaa aataatctgc aaattattac cagcttattg
     5761 cggctgcaaa aacaaacgat gccggaagat gcgcaaccaa aatttgagga ggccatcaat
     5821 cggatcatga caatgtcgct cattcaccgg aaattatacc aatcggccga ttttacacgc
     5881 atcgatatcg gtgcctatat caatgacctc attcgagaaa tagtgcaaag tcattccgta
     5941 aatgataatg taaaagcaga aatcaaatca gaatacaata agatcggact caactccatt
     6001 gttccccttg gattaatcat caatgagttg ttaagcaatt cgtttaaaca tgcgttttcc
     6061 aatggaaaaa gcggtgcaat caatgttgaa attagtggtg gtccgggtaa tacattttac
     6121 ctgaaataca gcgatacagg tatttggatt gatgaggaca gggaaggaaa ttccttcggt
     6181 ctggatttga ttgcaacctt aacagaacaa cttgaaggta gctttaccag aagcggatcg
     6241 agctatcatt ttaccttcaa taatcaggat agcaacacct gatttttgtg ctttttaggg
     6301 ctttgaccgg ttaagaaata cccaaacttt ttcattcgcg gtacttcatt tagggtaaat
     6361 ggtggagaat gatgatcaat tctcagtatt ttcgtaacat gaatagttta cgtcttttgg
     6421 ctttactgcc gcttttatgc acattgattt tcttttcctg caacagcaat gaaactgggg
     6481 aggtgattgc gaataaagac agtattccac agaatgattc gctgaacgag caacagcgtt
     6541 tgattgcgga tttagataag cgtattgttt ctgatccgga taatcccaat cattaccacg
     6601 cccgtgcctt cgcttatttt cgtcttgcac aatattccga agcaatgcag gatattgaac
     6661 gctgtatccg tatcgattca actattgcca tttttcacta taccaaaggc gagatattgt
     6721 ttgccgaact gaaagtagaa gaagccctgc agtcgtaccg cgctgcatta cgacaaaacc
     6781 caaattattt cgatgcggaa tttcgtattg gcagaattca catgtacata aaaagctgga
     6841 atgaggcgat gaaacatatc aatgcaggat taaaactcaa tcccaatttt gcagagggtt
     6901 atttcatgaa aggagaagtg tatgaagagt tacgtgatac cgctaaggca gcatcttctt
     6961 tccaaaccgc agtggagcag gatccgaatt attacgaggc ctatatccga ctcggcttgt
     7021 tgtatgccaa tgcgcacaac aaactggctc tggattatta taattctgca ctcagcattc
     7081 gacccaattc tgttgaggcg ctctacgata aagccatttt tatgcaggat cacggaatga
     7141 ttgctgatgc tttatctacc tatgataaaa tcattgaaat caatccccgc ctggaattac
     7201 cctggttcaa caaaggatat attcatttgg tggaaacggc caattacaat gaagcgttgg
     7261 taatgtttga taaagctatt gagatcaatc cacggtatat cgatgcttgg cacaatcgtg
     7321 gattgaccta tgaaggatta aaaaattatc ccaaagcaag agagaatttt aagaaagcat
     7381 tggaacttga tcccacatac gatttatcag ccgaagcaat ggatcgcctc gacagaatga
     7441 aaaagtgata tccggaatgg agtttacttt tgagctttgt tttcattagc cgggaacaaa
     7501 cactattgag aaaatagaga ttaattattc tgaaatcagt ggttcaagtc caaagatttc
     7561 atcttaccca aaaacgatta caaatgcgtt attatttgat tgtgtttttc ctcttgcaga
     7621 atatgatatc ctattcacaa attagggtag aggattttta taaaaagtcg gatggaaaag
     7681 atgatgcacc ttccattcaa cgtgccatgc gacacatcga ttcactgggt agagggagtg
     7741 ttgaatttga aggcagtaaa aaatacttgc ttcaatcggc agtggaatta ccacgttatg
     7801 ctgtgggtgg aagaaggatt attattctca acggaaacgg ttgcgagatc gttgggaaat
     7861 cgggaatcga cattttcaga aggattccta ttgatcagga tgaagcgctg aataaaatga
     7921 tggcgacacg ttttgtattc aacgattttt catttacagg tggtagcaaa gccatcaatc
     7981 ttggtgcaac gtacggaagc agtattcaac gctgtaattt tgttacacag gaagaagctg
     8041 ccatcgatat tcaatttgga cttgcaactg aaattcacca ttgcatactt accaatccaa
     8101 aaaaagacgg gatcgtattg cgatgcggaa ctgattgggg aggaagtacc aataattcgc
     8161 aatcgaactc atcggtaatc agtatgtgca gagcgtatgc aacaaaatcg actgatgctg
     8221 tttttcgaat tctgggatcg ggtggagtag tgctgcgcga ttgcatttcg gaaggatcgg
     8281 gagatgcgca gtacagcgtg ttcttcgacc ggcaaaacag tacaacggtg cgattgtttc
     8341 gcgtcgaaaa ttttcatttg gaacacgcac ctaaaaaggc aggaatttat ttaaatcaca
     8401 ccggaattgc caccatcgac ggattgtttg cgcaaatggc ctataaagat tttccgctgg
     8461 taatgagcgc tcccaatgca gaacaaataa cactgataaa tattccgcat tttgtgggag
     8521 gaatggtgct gtattcagga aataacgaag tcccatggcg acttgaatac tgccacaaat
     8581 cattctacgc aaaagaaaac tggagaatta acacaagcaa aggcgtggaa acgaaattgc
     8641 ctttttattt ctcaggaatt ggaggaaaat tccagatcaa gcaggagtat aaatagaagg
     8701 taagggttgt tagtaatcgg ttattagtta ttagttaata gttaatcacg aaaactgcct
     8761 gaattgccct attttcaggc tctccaaagg gggctgattc ctcagggtaa caaactggta
     8821 acaacaactg cataactgcg cctaaaattg ttacctgatc actctttagg tttctttctg
     8881 gaggcttaaa ttgctcattg cttcccggca atctttcaaa gaatcactta gcaaacatag
     8941 taaatttttt ggcatgtctg cgcttaccct tggatagtta ttgaaagcag caatgaaaaa
     9001 ataatactga aaatacagcc tccttttcat tcatacaagt tatatattcg gatgaattgg
     9061 agaattcagc aaagtttatc aatggcatgg caggatgtta aattgaattt tactttaagg
     9121 tcataagtaa acccatctaa atccgggaaa atactctcat gtctaatacc aagtaggtat
     9181 aatttttttt gaatctcact tgatactttt ttgctaaact gaattctaat taaggaattt
     9241 gcttcagaaa acggaaatcc gacatgaaat tccttacttg gatcaggttg aatagaaaac
     9301 aatccgcact gaccggaaat tctttttgta acatgcattg gataaaacaa cccatgatgt
     9361 tcatacgaaa aaggattagc ttctacatca gtattgatat agttgcaagt atgcatacaa
     9421 taaatagtgc ctccgttctc attacaattc tcaattgtgc catctggctt taatgacggc
     9481 ttagtggcaa agtaaagtgc tattaacggt gatgaagtcc aatcgagtag ccgtgttggc
     9541 aagccatgat gttgagccaa cgccagccat tcccaatcat tgtgcggctt aggatttaca
     9601 tctgcatttg cacgtttttt gaatcgagcc aaagtttcaa attcatattg ttttaaatca
     9661 aagcccgtag cttcttcttt tattctgccg aatgatggga ttagtttatg gttcttttta
     9721 tcagcgattc ctctgaaaac gacatggcca caatttaatt catcagacag taaaacttgg
     9781 atgatttcca aaaaagattt ggcatttatt attttgattt tccttttcgg cataaattca
     9841 atttagattt gttttttcat catcagtcgc agatttattt ttcatttcct cataaatctt
     9901 tattgcttcg ggatctttcg catatacttc catattataa accaaatcaa gaaattcatt
     9961 cacgatctta taactttttt tatattgctt ttcatcaact ttatctgggg attcccactg
    10021 tgcatttcct ttgttaaaac cgacataaaa gcccttgttc ttcatgttat ctgcctgatt
    10081 ccaccattcc tcattgaatt caattttcgg atcacctttc aaccaaccaa ttaaatcttg
    10141 aataccatct ttttggtgac ccctcaattc ttcaatactc ggtctgtctt cgccctttaa
    10201 gattgcagat tttactctac c
//
LOCUS       DAOMYG010000047        10138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2207_length_10138_cov_9.480313, whole genome shotgun sequence.
ACCESSION   DAOMYG010000047 DAOMYG010000000
VERSION     DAOMYG010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10138
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2207_length_10138_cov_9.480313"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(742..1269)
                     /locus_tag="PK610_02610"
     CDS             complement(742..1269)
                     /locus_tag="PK610_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRJ34845.1"
                     /translation="MSFADIFLIIPILTGTWLGFRNGFILTLTTLLAFTLASYAAIWF
                     SGAISGWILSMMSSPNEYVPLIAFSICFIAVCALVFFVGKWTSRLAKLVLLGSIDKLA
                     GAVFGFIQFTLITAVLMSVVDSYLTRKTGEAPQWREHSLLYEPLSSLSSFVLPGLEEQ
                     LQIVYTLEEITEKPG"
     gene            complement(1269..2282)
                     /locus_tag="PK610_02615"
     CDS             complement(1269..2282)
                     /locus_tag="PK610_02615"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HRJ34846.1"
                     /translation="MAKHIVFIFTVLMITLSSCEEGTNYKMTSGGAPGEVIVVVDDEL
                     WKSHTLGDTIRWFLEDIVPGLPQQEYMFNIGQYDPDRFSRVMRSHRNIILFEISQKVK
                     NPRAEYLRDSWAANQIVIKLHAPDAEAATALFVAESNKIVSLLNSTERERLIKRHKNE
                     NSGSIEATLKDKHNILMNIPVDFRLAEDKGNFIWLARDRIRYVSGEAHDVDEGIFIYY
                     YPYTNDSTFSSDFLLRVRDSVCKANVPGPSEGSYMSTERDSMYYPIFTATTHNKKYAA
                     EIRGLYRTVNDFYGGPFISITTLDEARNRIVTVDCFVFAPKFHKREYLREMEAICYSL
                     NFP"
     gene            complement(2275..3777)
                     /locus_tag="PK610_02620"
     CDS             complement(2275..3777)
                     /locus_tag="PK610_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRJ34847.1"
                     /translation="MKKLTGLVLAIVVSTSSLQAGDGADSSRIRKDDPIVAMIDSLMT
                     LKFLDSKSYAVSKNNKYNFPADSVPRYSESIYRQRIKKMDARTPMEFAYNENVQAYID
                     LYAVRYRKYTQIILGMTETYFPMFEQMLDKYGIPLELKHLPIVESALNPKAKSPAGAM
                     GLWQFMLPTGKMFGLEVNSMIDERCDPIKATEAACKYLKYLHSLYNDWNMALAAYNAG
                     PGTVNNAIRRSGGKTTYWEIYNYLPRETQGYVPAFIGVNYVFSHAPEHNLYPVEPRKR
                     YFEYDTVMVDRELDMTSISVILGITMDELKFLNPVYKTNVIPKFENKKSYLYLPKDLV
                     GDFLANEDTIYKHKHRPEAEATIASQKVHVVKSGEYLSTIASRYNVSVSDLKAWNNLK
                     SNSVSKGQRLVIRSGETAPTQENTASNNTAGSTVNNTKTTTANSSTKTSTNTSKGGAT
                     KTYTVQNGDSLWLIAQKHGVSVEEIKAQNNLRGTSLQAGQKLKIPAKGNG"
     gene            complement(3941..5371)
                     /gene="gatA"
                     /locus_tag="PK610_02625"
     CDS             complement(3941..5371)
                     /gene="gatA"
                     /locus_tag="PK610_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRJ34848.1"
                     /translation="MSYPSFTSIRNDLRSGKTTVVELVEDYLRNIEEKKHLNAFTEVF
                     RESALKKAAEVDGKLKSGTAGKLAGMVIGLKDNICYKGHKVSASSHILEGFESLYNST
                     ATERLLQEDAVIIGRLNCDEFAMGSSNETSYYGKVLNPLNEKCVPGGSSGGSAAAVAA
                     GLCTATLGSDTGGSIRQPASFTGTVGLKPTYGRVSRYGLLAYASSFDQIGPITHTVED
                     AALLLEVIAGADDFDSTVSHKPVPEYSAKLDFNGKARIAYIRECIESPGLNEEVRKGI
                     EKQIAQLKAAGHTVEAVDFPYLDFLVPTYYVLTTAEASSNLARYDGVHYGYRSAKSND
                     VESTYRLSRTEGFGKEVKRRIMLGTFVLSAGYYDAYYTKGLKVRRIIREKTREILSSY
                     DFILTPTTPDAAFEFGQKSADPVAMYLEDIFTVQANLAGVPAISLPLGKKSDGLPYGI
                     QLMAGDFEEEKLLAFSRLLESARQSC"
     gene            complement(5387..5581)
                     /gene="tatA"
                     /locus_tag="PK610_02630"
     CDS             complement(5387..5581)
                     /gene="tatA"
                     /locus_tag="PK610_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRJ34849.1"
                     /translation="MNLSILLGVIGPWQVILIVVVVLLLFGGKKIPELMRGLGQGIRE
                     FKDASKGEDKNTNTPSNTGN"
     gene            complement(5642..7006)
                     /locus_tag="PK610_02635"
     CDS             complement(5642..7006)
                     /locus_tag="PK610_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRJ34850.1"
                     /translation="MATRTWNNYLVLLLLLAIGVNGVSAQKTSEKLTQQKRELEEKIK
                     NTKELIGTKKNIQRLTIAELAIINQQINYREELIHNISSQVRRLNQQIEDNRSVIESL
                     SEDLIRLKSQYSRMLFYAYKHRNTYHKLLFVFAAKDFNTGFLRVKYLKQFGDSRKRQV
                     ELINGTQTELERRNHDLGEKRKEKEVLANEQVKEKENYSKDKESQQQMLAQLQKEEKQ
                     LKQILDEQEEKKRLLAKQIQKALQEEIRKQQEEERKRKEEEERKRKAQQGNSGDVAKN
                     NEKTKTNTTTNTNGTTKTNTNKEKNFEGITPDGDPASTNFENNKGRLPWPVERGEFTQ
                     GFGNIPHPTLAGIMLSNNGVDIGTPKGSRVRSVFEGTVTSILVISGQGKVVIISHGAY
                     RTVYANLGEVYVSKGDKVKIKQDIGSLLSEEDSKISEVHFEIWKITETDMLKQDPTHW
                     LIKK"
     gene            complement(6984..7814)
                     /locus_tag="PK610_02640"
     CDS             complement(6984..7814)
                     /locus_tag="PK610_02640"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HRJ34851.1"
                     /translation="MNKAFHSVILILAVIVLSATACRTQKGLIRGKGKVENLSTKALI
                     DSIGKHCFSFHYFNSRISTEVFIEGEGSKSFKTHLKIRSDSCIWLTLSVANIVGASAL
                     VTVDSVKFTDKIKKEYFTEAFSYINETFGTEIDYHTLQEVLIGNLVYFDADAKYKTKE
                     DTAYYIISTVGKRKLKRAFENERVAKKEPFIYRYYILPGTYRPARMIINDLTDSTELD
                     IEYLSYEYVDSLLVPAEVKISASSPRKKAVIELKYSRTKVNEFTEFPFKIPDGYKNME
                     "
     gene            complement(7807..9525)
                     /locus_tag="PK610_02645"
     CDS             complement(7807..9525)
                     /locus_tag="PK610_02645"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ34852.1"
                     /translation="MRILLFAFVALQLFACKGQKNATSRNKGGDKDTLARIAAQAMLI
                     DGMTKKSSGDAAGALRDFEACIHYLPTCDAAYYHLSGLYMMQGRAEMAIASADRAAEL
                     DPKNEWYLLQVANISQKNGDLKKSISTYEKLIKLNPEKPDYYLPYSDVLLQSGQADKA
                     ILALEKLEKLRGYDEAVIFQKFQIYSSAKKYDKAIAEIEKMIVANPSEVRNYGIIAEL
                     YMEIGQTQKALDYYHKILGIDPDNGLVHLALADYQWQNNNPEGAFLELKKAFESIDLE
                     IDTKMKILLDYYDRPNDKELTAQAYELLEIVIKVHPTEGKAWSIYGDFLNRDKKHTEA
                     REKFRKAIEFERNYFAIWNQVVNLDAVLNDTESLFTDSKEAEALFPQQPGFYFYHALG
                     AYRKGKYNEALDALLAGKDLVVDNAALQFEFLQLLGDTYHKLGNHTESDNAYEQALGI
                     DPNNAYLLNNYAYYLCERRVKLEKAAAMSKKSNELEPNQSNFQDTYAWILFRQSKFAD
                     AEVWIKKALSSGGQESGTILEHYGDILFHTGKTEEAIIQWKKAKEKTGAGNNLDRKIR
                     DKKYYE"
     gene            complement(9522..>10138)
                     /locus_tag="PK610_02650"
     CDS             complement(9522..>10138)
                     /locus_tag="PK610_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRJ34853.1"
                     /translation="KVDDSSAFGVVKTDAAGTITDFVEKPKEFVSDLAIIGIYYFRDG
                     DNLKKELQFLIDNNIRGNNEFQLTDALENMKKKGTRFAAGTVEEWLDCGNKNVTVYTN
                     QRVLENNKEKMKLPASLKNENSIVIPPCYIGENVILRNSVIGPHVSIGEGSVIESSVI
                     SDSILQQKVSIQNASIARSMIGNFAEYKGKASDLSIGDYTQITA"
BASE COUNT         2531 a         2217 c         2308 g         3082 t
ORIGIN      
        1 gtggagctat gacaccaagg tgctatgcaa aggtgtacca tagatgattg tgctaaaaat
       61 tggaaatgga atataaattg aagtcagcac caattcgatt tttagctgta tttccattgg
      121 tacaccttag cgtaacgcct taggcgtctc tttgggctga gcgacctgcc ccgaccgata
      181 gcgtcgggaa gcgtagggga gtcgaagcct tgctccactt gaaaaaacct gaagtagtag
      241 aacgttttta aattcataaa acggaaaaca cattaacgcg aatcaaaaca aagaaacttt
      301 gcggtacaac cttagtgatt cttagtgctc ttagtggcta agtggttaag taaaatcaac
      361 atcggtaccc cttagcgtaa tgccgtaggc gcctagctcc cctttaaaaa atcttggtta
      421 gtaggccaaa taaaattcag gttccggatt aattatgaat tcaccagaca aaccaacttt
      481 ctttgcggtt aaaatcctta gtgtttctta gtgcacttag tgtcttcttg tccgccgaag
      541 gaggaagtgg ttaagtaaaa tctccagtgg tacccctttg cgcaatgcca aaaggcatct
      601 ttgctcccct tgtgaaaatc taaaatgata taaccggtaa tggtcaaaac acggattaac
      661 cttattctgg atccaactaa aaaaatcttt gcggtaaaac cttatgtgtg attggtaaca
      721 tgttaaaggt gaaagtgcct tttagcccgg tttttcagtg atctcctcca gagtataaac
      781 aatctgtaat tgctcttcca gccctggtag cacaaaagaa ctaagggaac tgagtggttc
      841 gtacaagagc gaatgttctc tccattgggg tgcttcaccg gttttgcggg tgaggtacga
      901 atccactacc gacatcaata cagcagtgat cagcgtaaac tgaatgaatc cgaaaactgc
      961 tccggcaagt ttatcgatgg atccgagcaa caccagctta gccagtcgtg atgtccattt
     1021 gccaacaaaa aacaccagcg cgcatactgc aataaagcat attgaaaatg ctatgagtgg
     1081 aacatactcg ttgggcgaac tcatcatcga gaggatccat cctgaaatag ccccgctgaa
     1141 ccaaatggcg gcataactcg ccagcgtaaa ggcgagcagg gtggttagtg taagaatgaa
     1201 tccgttgcgg aatcccagcc aggtgccggt gagaatgggg atgattagaa aaatgtcagc
     1261 aaacgacatc agggaaagtt gagcgaatag cagatcgctt ccatctcacg gaggtattcg
     1321 cgtttatgga atttgggtgc aaacacaaaa caatccaccg taacaatacg attacgcgct
     1381 tcatccaatg tggttatgga aataaatggt ccgccgtaaa aatcattcac tgttcggtac
     1441 agaccacgaa tttctgctgc gtatttttta ttgtgcgttg ttgcagtaaa aatcgggtaa
     1501 tacatgctgt cgcgctctgt gctcatgtac gaaccttcgc ttggaccggg aacatttgcc
     1561 ttgcaaacag aatcacgtac gcgaagcaga aaatcggagc tgaaagtgct gtcgttagtg
     1621 taggggtagt aataaatgaa aattccttcg tcaacatcat gcgcttcgcc gcttacataa
     1681 cggatgcgat cgcgtgcaag ccagatgaaa tttcctttgt cctctgccag acgaaaatcg
     1741 acagggatat tcatcaggat gttgtgtttg tctttcaatg tggcttcaat acttcctgag
     1801 ttttcgtttt tatgtcgctt gatgagacgt tcgcgctcgg tggagtttag cagcgaaacg
     1861 atcttattgc tctcggcaac aaacaaggca gttgcagcct ctgcatcggg tgcgtgaagt
     1921 ttaattacga tctgattggc agcccatgag tcgcgcaaat attcggctct gggattcttt
     1981 actttctgac tgatctcaaa cagaataatg ttgcggtgac tcctcatcac ccgcgaaaaa
     2041 cgatcgggat cgtactggcc aatgttgaac atgtactcct gctgtggcaa accgggtacg
     2101 atatcttcca ggaaccagcg aatggtatcg ccaagtgtat gtgatttcca aagttcatca
     2161 tccaccacta caatcacttc accgggcgca ccgccggagg tcattttgta gttggtgcct
     2221 tcctcgcagg aggaaagcgt gatcatgagt acagtaaaaa tgaaaacgat gtgcttagcc
     2281 attgcctttt gccggaattt ttaatttctg accggcctgc aaactggttc ctctcaggtt
     2341 gttttgtgcc ttgatctctt ctacactcac tccatgcttt tgtgcgatca gccaaagtga
     2401 atcaccgttt tgtacagtgt aggttttggt tgcgccacct ttagaggtgt tagtagaggt
     2461 tttcgtggac gagtttgcgg tggtcgtctt agtgttgtta acggtgctac cggctgtgtt
     2521 attgcttgca gtgttttctt gtgtaggagc ggtctcgccg gaacggataa cgagacgctg
     2581 acctttactc acgctgttcg attttaaatt gttccaggct ttgaggtcgg atacactaac
     2641 gttgtaacgc gatgcaatgg tgctgaggta ttcgcctgat tttaccacgt gtactttctg
     2701 actggcaatg gtggcttcgg cttccggacg atgtttgtgt ttgtaaatgg tgtcttcatt
     2761 ggcaagaaaa tcgccaacaa gatctttagg taaatacaaa tacgattttt tattctcaaa
     2821 ctttggaatc acattcgttt tgtaaacagg attcaaaaat ttcaattcat ccatcgttat
     2881 accgagaatg accgaaatcg atgtcatatc cagttcacga tcgaccatta ccgtgtcgta
     2941 ttcgaaatac cgtttgcgcg gttctaccgg atacaggttg tgttcgggtg catgcgaaaa
     3001 aacgtaattg actccgatga atgcaggaac atatccttgt gtttcgcggg gcaggtagtt
     3061 gtagatttcc caataggtag ttttcccccc cgatctgcga atggcattgt tcactgtgcc
     3121 cggaccagca ttgtaggcgg caagcgccat gttccagtcg ttatagagtg agtgcagata
     3181 tttgaggtat ttgcaggcag cttcagtcgc tttgataggg tcgcaacgtt catctatcat
     3241 cgaattcact tctagtccga acatttttcc ggtaggtaac atgaattgcc agagccccat
     3301 tgcgcccgca ggcgattttg cctttggatt cagtgccgat tcaacaatgg gaagatgttt
     3361 cagctcaagg ggaattccat acttgtcaag catctgctca aacatgggaa aatacgtctc
     3421 cgtcattccc aaaatgatct gagtgtactt gcggtaacga acggcataca aatcgatata
     3481 agcttgtaca ttttcgttgt aggcaaactc cattggagtg cgtgcatcca tttttttgat
     3541 gcgctgacgg taaatgcttt cactgtaacg cggaacgctg tctgcaggga aattgtattt
     3601 gttgtttttg ctgacagcgt aactcttgct gtcgaggaat ttcaatgtca ttaaactatc
     3661 gatcattgct acgatcgggt cgtctttacg aatacgactg ctatccgcac catctccggc
     3721 ctggagactg cttgtgctta ccactatggc aagaacaagc cctgtcagtt ttttcatgct
     3781 tacctggttt tataaatggt tatacccgat gtgccacaaa aagttccttg caagagcaaa
     3841 ttatgggacg tgaatataag aagaataatg aacaattgtt cataaccctg cgatgaaata
     3901 tcaagaaaat aatgagttaa tgagcctgta attcagttcc ttagcaggat tgccgcgcag
     3961 attccagcaa gcgggaaaaa gccagtaatt tttcttcttc aaaatctccg gccatcagct
     4021 gaatgccgta ggggagtccg tctgacttct tcccaagggg gagtgaaata gcgggcacac
     4081 ctgcaagatt ggcctgaacg gtgaaaatat cttcaagata catagcaaca ggatctgcac
     4141 ttttctgacc aaattcgaaa gctgcatccg gagtggttgg tgtaaggatg aaatcgtagc
     4201 tcgataaaat ctctctcgtt ttttcgcgaa tgatgcgtcg aacttttaaa ccttttgtat
     4261 aataagcatc gtaataccct gcactcaaca caaaagttcc aagcataata cggcgcttca
     4321 cttctttccc aaatccctcg gtgcgcgaaa gtcggtaagt gctttccaca tcattgcttt
     4381 ttgcacttct gtagccataa tgaactccgt cgtaacgggc aagattgctg gatgcttcgg
     4441 cagtggtgag tacgtaatag gtggggacaa ggaaatcgag gtaagggaaa tcaaccgcct
     4501 caacggtatg accagcggcc ttgagttggg caatttgctt ttcaattcct ttgcgaactt
     4561 cttcattcag ccccggactt tctatgcatt cgcggatata ggcaatgcgt gctttcccat
     4621 tgaaatccag tttcgcggag tattcaggaa cgggtttatg actaacggta ctgtcaaaat
     4681 catctgctcc tgcaatgact tccagcagta gtgcagcgtc ttcaacagtg tgggtaatag
     4741 gaccaatctg atcgaaagag gaagcataag cgagcaatcc gtaacgcgaa actcttccgt
     4801 aggttggttt tagtcccaca gtacctgtaa aggatgcggg ttggcgaatg gatccgcctg
     4861 tatcggaacc caaggtggca gtgcataatc cggcagcaac ggcagctgca gatcctcctg
     4921 aggaacctcc gggaacgcat ttttcgttga ggggattcaa tactttgcca tagtaagatg
     4981 tttcattgga agagcccatt gcaaattcat cgcaattgag tcggccgatg ataacggcat
     5041 cttcctgcag aagacgttcg gtagcagttg aattgtagag ggattcaaat ccttcaagga
     5101 tatgcgaaga ggcagaaacc ttatgacctt tgtaacagat attgtccttg aggccaatca
     5161 ccattcctgc aagttttcct gcagtgccgg atttaagttt gccatcgact tcggctgctt
     5221 ttttcagcgc actttcacga aagacttccg taaaagcgtt gaggtgtttt ttttcttcaa
     5281 tattgcgcaa atagtcctct accagctcca caacagtggt tttgccggaa cgaagatcgt
     5341 tgcggatgga tgtaaaagaa gggtaggaca ttgttagctg accggattaa tttccggtgt
     5401 tcgacggggt attggtgttc ttgtcttctc ccttggaagc gtccttaaac tcacgaatac
     5461 cttgaccaag tccgcgcatg agttcgggaa ttttttttcc gccgaaaagg agcagaacaa
     5521 ccacaacaat aaggatcacc tgccagggtc cgatcactcc taaaagaatg gatagattca
     5581 tctgaatgag aattattaac gcccaaaagt acgcaattca ggacaagtga agccgaaaag
     5641 cttatttttt gatgagccag tgcgtaggat cctgcttgag catgtcggtt tctgtgattt
     5701 tccagatctc aaaatgaacc tcagaaatct tcgaatcctc ctcgctgagt aaactgccga
     5761 tatcctgttt gatttttacc ttatctccct tacttacata gacttcaccc aaattggcat
     5821 agactgtacg gtaggccccg tgtgaaataa tgaccacttt tccctgacct gaaatgacca
     5881 gaatagaggt aactgtgccc tcaaaaacag agcgcaccct ggatcctttc ggtgtgccga
     5941 tatctacgcc attattgctc agcatgatgc ctgcaagggt tggatgcgga atatttccaa
     6001 agccttgtgt gaactcaccc cgttctaccg gccagggtag tcgtcctttg ttattttcaa
     6061 aattggtcga tgcaggatct ccatccggtg ttatgccctc aaaatttttc tccttgttgg
     6121 tgttggtctt ggtagttccg ttggtgttgg tagtggtgtt ggtttttgtc ttttcattgt
     6181 ttttagcaac atcacccgaa tttccctgct gcgcttttcg tttgcgttct tcttcttctt
     6241 tgcgcttacg ttcttcctct tgttgcttgc gtatttcttc ctgcagtgct ttttgaatct
     6301 gcttggcaag aagtcgtttt ttctcttcct gctcgtcgag aatttgcttt aactgttttt
     6361 cttccttttg caattgagca agcatttgtt gctgactttc cttatccttc gaataatttt
     6421 ctttttcttt tacctgttca ttggcgagca cttctttttc ttttcgcttt tcaccgagat
     6481 cgtggttgcg gcgttcgagt tctgtttgtg ttccgttaat gagttcgacc tgacgtttac
     6541 ggctgtcgcc aaattgcttc aggtatttca ccctcaaaaa tccggtattg aaatcttttg
     6601 cagcaaaaac aaacaggagt ttgtgatagg tgttgcggtg tttgtaagcg taaaacaaca
     6661 tccgtgaata ctgcgatttt aaccggataa gatcttcact cagcgattcg ataacactac
     6721 ggttgtcttc aatttgctgg ttgagtcggc gtacctgcga tgagatattg tgaatgagtt
     6781 cttcgcggta attgatctgc tgattgatga tggctaattc ggcaatggta agacgttgaa
     6841 tgttcttctt cgtaccgatc agttccttcg tgtttttgat tttttcctcg agttcccgct
     6901 tttgttgcgt tagtttttca gacgttttct gggccgaaac accatttacg ccaatcgcta
     6961 aaagcaatag caaaaccaga taattattcc atgttcttgt agccatcggg gatcttaaac
     7021 gggaattctg tgaattcgtt cacttttgtg cgcgaatatt tgagttcaat cactgctttt
     7081 tttcttggtg agctggcaga gatcttcact tcagcaggaa ccaaaagcga atcgacgtac
     7141 tcgtaactga ggtattctat atcgagttcg gtagaatcgg taaggtcgtt aatgatcatt
     7201 cgcgccggac gataagtgcc tggaaggatg taatagcggt agataaaggg ttcttttttg
     7261 gccacacgct cattctcgaa tgcacgcttc agttttcgtt ttccaacggt actgatgatg
     7321 taataggcag tgtcttcttt ggttttgtat ttggcgtcgg cgtcgaaata caccaggttg
     7381 ccaattaata cttcctgcag tgtgtgataa tcgatctcgg taccaaacgt ttcgttgatg
     7441 taactgaatg cttcggtaaa atattctttt ttgattttgt cggtgaactt tactgaatca
     7501 acagttacca atgcgcttgc gcccacaatg ttggctactg atagggtgag ccagatacaa
     7561 ctgtcgctgc ggattttgag gtgggttttg aatgatttac tgccctcgcc ttcgatgaac
     7621 acctctgttg aaatgcgtga attgaaataa tgaaatgaaa agcaatgctt gccgatgctg
     7681 tcgatcagcg cttttgtgct gaggttctct acttttcctt taccgcgaat caaacctttt
     7741 tgcgttcggc atgctgttgc cgacaaaaca ataactgcaa gaatcagtat gacgctatga
     7801 aatgctttat tcataatatt ttttatcccg gatcttgcga tcaagattgt tgccggctcc
     7861 tgttttttct tttgcttttt tccattgaat tatggcctcc tccgttttgc ctgtgtggaa
     7921 gagaatatca ccgtaatgct caagaatagt gccgctttcc tgcccgccac tgcttaatgc
     7981 tttttttatc cagacctcgg catcggcaaa tttcgattgc cggaagagaa tccatgcata
     8041 ggtatcctga aaattgctct gattgggctc cagctcattg ctttttttcg acatcgctgc
     8101 tgccttttca agttttaccc gacgttcgca taagtaatag gcataattgt tcagcaaata
     8161 agcattgttc ggatcgatgc ctaatgcctg ctcatacgca ttatcgcttt cggtgtggtt
     8221 gcccagtttg tggtaggtat cgcccaataa ttgtaaaaac tcaaactgca gtgctgcatt
     8281 gtctaccacc aggtcttttc ctgccaggag tgcatcaagt gcttcgttgt atttgccctt
     8341 acggtaagca cccaatgcgt gataaaaata gaatccgggt tgctgcggaa ataatgcctc
     8401 tgcttctttg ctgtctgtga agagcgattc ggtgtcgttc aatacagcgt caagattgac
     8461 tacctgattc caaatggcaa aatagttgcg ctcaaattca atggcctttc tgaatttttc
     8521 ccgggcttcg gtgtgctttt tatcgcgatt cagaaaatca ccgtaaatgc tccatgcttt
     8581 cccctcagtg ggatgaactt taattacaat ctccagtaac tcataagcct gtgcagtgag
     8641 ctctttgtcg ttgggacgat cataataatc cagcagaatt ttcatcttgg tgtcgatctc
     8701 cagatcgatg ctttcaaatg cttttttcaa ttccagaaat gcaccttcgg ggttgttgtt
     8761 ttgccattgg taatcggcga gcgcaagatg tacaagtccg ttatccggat caatgcccag
     8821 gattttatgg taatagtcga gtgctttttg ggtctgacca atttccatat agagctcggc
     8881 aataattccg tagttgcgga cttccgacgg attggcgacg atcatttttt caatttccgc
     8941 aatggctttg tcgtattttt ttgcagaaga atagatctgg aatttttgaa atattactgc
     9001 ttcgtcgtaa ccacgaagtt tctccagttt ttccagcgca agaatggctt tgtccgcttg
     9061 tcctgactgc agcaaaacat cagagtaggg gaggtaataa tcgggctttt caggattgag
     9121 ttttatgagt ttttcatagg tggaaatgct ttttttcaga tcgccgtttt tctgactgat
     9181 attggcaacc tgcagtaaat accattcgtt tttcggatct agttcagcgg cgcgatctgc
     9241 gcttgcaatg gccatttcgg ctcttccctg catcatgtac aatcccgaca aatggtaata
     9301 tgccgcatcg caggtgggta aatagtgaat gcatgcctca aaatcgcgca aagcaccggc
     9361 agcatcaccg gacgattttt tagtcattcc atcaatcagc atggcctgag ccgcaatacg
     9421 ggcaagcgtg tctttgtcgc cacccttgtt gcgggaggtt gcgttctttt gacctttaca
     9481 ggcgaaaagc tgaagagcga caaaagcgaa aagaagtatt ctcatgctgt tatctgggtg
     9541 taatcgccga tgctaagatc acttgccttg cctttgtact cggcgaagtt tccgatcatt
     9601 gaacgggcaa tgcttgcgtt ttgaatgctg actttttgtt gaagaatgct gtcgctgatc
     9661 acactgcttt caataacgct tccttctcca atcgacacgt gtggaccaat tacgctgttg
     9721 cgcaggatca cgttttcgcc aatgtaacag ggaggaataa cgatactgtt ttcatttttg
     9781 agcgatgcag gcaatttcat tttttcttta ttgttttcga gaacacgctg attggtgtaa
     9841 acggttacgt ttttgttccc gcaatcgagc cattcctcaa cggtaccggc agcaaaacgg
     9901 gtgcctttct ttttcatgtt ttccagagcg tctgttaact gaaactcatt attgccacga
     9961 atgttgttat caatgaggaa ttgcagctct tttttcagat tgtctccatc gcggaaataa
    10021 taaattccga tgatagccag atcggaaaca aattccttgg gcttttccac gaaatcagta
    10081 attgtgccgg cagcatctgt tttaaccact ccaaaagcag aggaatcatc aactttct
//
LOCUS       DAOMYG010000048        10099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2219_length_10099_cov_8.156511, whole genome shotgun sequence.
ACCESSION   DAOMYG010000048 DAOMYG010000000
VERSION     DAOMYG010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10099
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2219_length_10099_cov_8.156511"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PK610_02655"
     CDS             <1..602
                     /locus_tag="PK610_02655"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRJ34854.1"
                     /translation="DSLLDAFEKVPRHAFLGDNAFDEHAYEEKAFHIGAGQTISNPYT
                     VAFQTHVLDVVKGEKILEIGTGCGYQTAILCQLGAKVFSIERQRELFEKARITLNQMN
                     FSPRLFFGDGFKGQPAFAPYDKIIVTCGAPFIPQDLVNQLKPGGIMIIPVGENEQEMT
                     WIMKNPDGSLEIKELGKFKFVPMLEEKQRSNDKLFFRKP"
     gene            771..1646
                     /locus_tag="PK610_02660"
     CDS             771..1646
                     /locus_tag="PK610_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34855.1"
                     /translation="MKKNIFILGLALATTTAFAQDLTSKKGENYLPEAGDIAISFDAA
                     PFLNYVGGFFSNAGGTAPTGNYVGNLPWHIRGKYFASETMAFRGGLRLNFGGDTYTAM
                     VGQSGAAAPSFPAAPAMVEDVYKAGGSNIVLSGGVEMRRGKTRLQGYYGGELFIVSGG
                     TKDTYTYGNAMSTTAAYPTTNFAFVGGNNLVNDPFYGLPGRVTESKTSSFGFGLRGFI
                     GAEYFIFPKISLGLEYGWGLMMGSGKSTRSIETQNGTAVGVVVDETATKSKSFGFDSD
                     INYGTNGQLNIIFHF"
     gene            1790..2935
                     /locus_tag="PK610_02665"
     CDS             1790..2935
                     /locus_tag="PK610_02665"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019668180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HRJ34856.1"
                     /translation="MSVPFYNPAYRVGILGGGQLGRMLIQEAVNLNISISILDPDPSA
                     PCKNLVGEFVCGSLNDYDTVMRFGADKDLLTIEIEHVNVDALEHLEKQGKKVYPQPHV
                     LRMIQDKGEQKKFYQQNNIPTAPFYLVENRAGIALHGAEFPFFQKLRKGGYDGKGVTR
                     LDDVNELSKAFDEPSVLEKLVPFKKEISVIVSRNENGEIAHFPVVECEFNPIANLVEF
                     LFSPSDISSKTEHDAIRIAREIAECSGIVGLLAVEMFVLDDDTVLVNEIAPRPHNSGH
                     HTIEANISSQYEQHLRAILNLPPGSTDIVRPAIMVNLLGEDGYTGDAVYKGLEDILRI
                     PGVFVHLYGKKTTKPFRKMGHVTILADNISAARSLARKVQSTLKIIA"
     gene            2939..3427
                     /gene="purE"
                     /locus_tag="PK610_02670"
     CDS             2939..3427
                     /gene="purE"
                     /locus_tag="PK610_02670"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRJ34857.1"
                     /translation="MSVHVGIIMGSQSDLPVMQEAAEMLKEFGIQFEMTIVSAHRTPE
                     RMFDYAKTAAQRGLKVIIAGAGGAAHLPGMTASLTALPVIGVPIKSSNSIDGWDSVLS
                     ILQMPGGVPVATVALNGAKNAGILAAQIVGSGDAAVLKKIEAYKEGLKSKVLEMAKKL
                     EG"
     gene            complement(3485..5590)
                     /locus_tag="PK610_02675"
     CDS             complement(3485..5590)
                     /locus_tag="PK610_02675"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ34858.1"
                     /translation="MRKLDYCLLFVFVIISGNRAFCVPQTELDSMRAVNVKSLTDSLK
                     ARHEFLLGKKWEDTSSDSAVAAYKRSINYHHRSLVAFLEPHEMEELARVYLRLGLNYK
                     NAGQIHDAKLEYEKALFLFEYLQSRVGVAECVNNIGLVYYQLGELGTTLNYLYRSLYL
                     LESAGELKNSAYCYLNIASVQEEMGEDERALESYLACLALLEEINDPLGLSYVHNNLS
                     YLYCKMKAFQQAIEHAARSYSYAEQNGDKRSMSYSLANMGTVYLDLGDHDRAIEYFEK
                     GLQLREELGDAAGIVYAYDNIAEVYRDRGEFSKAIELGEKAYKKAVELGYPEIMSRTS
                     KIMYMIYEKKGDPAKAFPFYKMHITMRDSLQNQKNRDNALRQEMLMNFGKRETEFKKE
                     QEAQQLLHAEESKRQRLILSGVVVLLVIIALSAWMFYNRFKVSQQQKKEIERQKLLVD
                     EKNREILDSINYAKRLQQAILPPVNDFSRLLGDAFVLYLPKDIVAGDFYFLETKEEEV
                     LFAAADCTGHGVPGAMVSVVCANALNRVVKELGETDPGIVLDETARLVEATFSKNQGD
                     VWDGMDISLCSWRKGDSILKWAGANNGIWIFRENEIIEVKPDKQPIGKFQGRKSFLSH
                     QIEIRKNDIIVLFTDGFADQFGGPGGKKMKYKNVQKSISANLHLSMNELGEKLNQEFF
                     QWKGDLEQIDDVTMIGIRI"
     gene            complement(5587..7809)
                     /locus_tag="PK610_02680"
     CDS             complement(5587..7809)
                     /locus_tag="PK610_02680"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ34859.1"
                     /translation="MNRVFLFSLRLLYVFALPAGTAYAQNAKIDSLQKQLNKTTDPIT
                     RVYILDELGWEYLYIDPDKTIEYVQQSFAIHTDPKYFDQNSQVSQVNRAAGYSYLGNA
                     YNIKGDYESSLRYHYKALALADSLKDDKRRSTMMISIGNTLNAMRSYDESIRYYYMAL
                     ELKIKLGDKKGEIRCLQNIGGVYLYTFQNDSAAYYMEKARDLAIQNGSDKSLIIPIET
                     NLGVVYKRQGKWKKAIDSYKWTVALRREGGEKYRLIQSLMNLGILYTEIDSIPQAMAA
                     LQEAYALVRNSNFDDIHMEVLRSLAKAEYLSGNSDSVYWHLIESNILSEKVFKASLSK
                     NATEMQSKYDLDKKQREIMLLSREQKIQQIEAIRQQQQISLLNSERARKEIELAANQE
                     KIKAKQNQLELLNKQKVLESNEAKAREAQLTAEAEKAEGENRRQRIIIFSVIIGLILS
                     LVSVFFIFRSLQQTRKQRRIIEQQKIQAEEQKELLQEKNKEILDSITYARRLQEAILP
                     PAEYISSLLPENFVIYFPKDIVAGDFYFLEQEKNKIIIAVADCTGHGVPGAMVSVVCS
                     NALNRVVKEFGETDPGKILDRTRALVMDTFRRSDQEVKDGMDISLCTIERKENEFTLQ
                     WAGANNPLWYLENDTMNEIAADKQPIGFSYEAHPFVTHTLSLKKGDAVFLFSDGYADQ
                     FGGPRGKKFKYRTLQEFISQRKSLSMPQLKDELVAAYREWKGEIEQIDDVSMIGIRLV
                     "
     gene            complement(7912..>10099)
                     /locus_tag="PK610_02685"
     CDS             complement(7912..>10099)
                     /locus_tag="PK610_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRJ34860.1"
                     /translation="AIMDKVVEKTNAPLKSGFEITREMSEKIFVIPPARTRYLSEISE
                     NNRKYDNWVIKQVEIADRLYGIDTAIKTFDEKKDADIVKTLRAQFDKIKLDLDPHNWE
                     LIQQWPDKVKRYKDEFYVFQVRDKQIKIKTHTESLSHTQVPKVATPRYKSWGDVLRWV
                     LRENVPGEFPYTAGIYPFKREGEDPTRMFAGEGCPERTNKRFHYVSLGMPAKRLSTAF
                     DSVTLYGNDPDLRPDIYGKIGNSGVSICCLDDAKKLYSGFDLSDPKTSVSMTINGPAP
                     MLLGFFMNAAIDQQCEKYIKANGLEAEISKKIDSIYQQRGVPRPKYNAPLPEGNDGLG
                     LMLLGVTGDMVLPADVYAKIKTETLMQVRGTVQADILKEDQAQNTCIFSTEFALRLMG
                     DVQEYFIQKKVRNFYSVSISGYHIAEAGANPITQLAFTLANGFTYVEYYLSRGMDINE
                     FGPNLSFFFSNGIDPEYAVIGRVARRIWAKAMKDKYGADARSQMLKYHIQTSGRSLHA
                     QEIDFNDIRTTLQALYAIYDNCNSLHTNAYDEAITTPTEESVRRAMAIQLIINRELGL
                     AKNENPIQGAFIIEEMTDLVEEAVLTEFDRITERGGVLGAMETMYQRSKIQEESLYYE
                     TLKHTGEFPIIGVNTFLSSKGSPTIIPAEVIRATEEEKQQQIDTIRFLHKKYETESKE
                     HLSELQQAAILNRNLFEGLMEATKYCSLGQITHALFEVGGQYRRNM"
BASE COUNT         2737 a         2273 c         2085 g         3004 t
ORIGIN      
        1 cggattcctt attggatgct ttcgaaaagg taccacgtca tgcctttttg ggggataatg
       61 cctttgacga acatgcctat gaagagaaag cctttcatat aggtgcagga caaaccattt
      121 cgaatcctta tacagtagcc tttcagacac atgtattgga tgtggtaaag ggcgagaaga
      181 tattggagat tggcacgggt tgcggttacc agactgccat tttatgccaa ttaggcgcca
      241 aagttttctc gattgagagg cagcgcgaac tttttgaaaa ggccaggatc acattaaatc
      301 aaatgaattt tagtccccgc ctcttttttg gggatgggtt taaaggtcag cctgcctttg
      361 ctccttatga taagataatt gtcacatgtg gggcaccctt tattcctcaa gaccttgtga
      421 atcaactcaa accaggcgga attatgatca ttccggtagg agaaaacgaa caggaaatga
      481 catggatcat gaaaaatcct gacggtagtc ttgaaatcaa ggagttggga aaatttaaat
      541 ttgtacccat gttggaagaa aaacagcggt caaatgataa attatttttc agaaaaccgt
      601 aaccttgcat tccaaacagc cgtaaaaagc gggtgttttc aacaaaaaat agttaacaac
      661 agtatgattg ttaataactt ggcacagcga aagaatgaca tgagcgggag aattgccaat
      721 tttggtacaa tatctgttac acaacgcgca aactcttaaa accaaataca atgaaaaaga
      781 acatctttat tcttggtctt gctctcgcga ctacaactgc gtttgctcag gatttgactt
      841 caaagaaggg tgaaaactac cttccagaag ctggtgacat tgccatttct tttgacgctg
      901 ctccattcct gaattacgtt ggtggattct tcagcaacgc tggtggaact gcacccacag
      961 gtaactacgt aggtaacctt ccttggcaca tccgtggtaa gtattttgct tctgaaacta
     1021 tggcattccg tggtggtctt cgcctgaact tcggtggtga cacttacact gcaatggttg
     1081 gtcagtctgg tgctgccgct ccttctttcc ctgcagctcc tgcaatggtt gaagacgttt
     1141 acaaagctgg tggatctaac atcgttcttt ccggtggtgt tgaaatgcgt cgtggtaaga
     1201 ctcgtcttca gggttactac ggtggtgaac ttttcatcgt ttctggaggt actaaggaca
     1261 cttacactta tggtaacgca atgagcacaa ctgctgcgta tcctacaact aactttgcgt
     1321 ttgttggtgg taacaacctg gtgaacgatc ctttctatgg tcttcctggt cgcgtaactg
     1381 aatctaagac atcttctttc ggattcggtc ttcgtggttt catcggtgct gaatacttca
     1441 tcttcccaaa gatctcttta ggtcttgagt acggttgggg tcttatgatg ggtagcggta
     1501 aatctactcg ctctatcgaa actcagaacg gtactgctgt aggtgtagtt gttgacgaaa
     1561 ctgctactaa gtctaagtct ttcggatttg actctgatat caactacggt actaacggtc
     1621 agttgaacat cattttccac ttctaatcaa cgattagatt ggtataaaag acccgcccaa
     1681 aaggcgggtc tttttttttg cattcattta ttccccattt tcattcaccc caggatggaa
     1741 atatctcctt acggtggtaa gtcctgatac aggatgctat ctttgcacta tgtctgtccc
     1801 tttttacaac cccgcttacc gggtaggaat tttaggcggt ggccaattgg gtcgcatgct
     1861 tattcaggaa gcggttaatc taaacattag catttccatt ctggatcctg atccctctgc
     1921 accctgtaag aatctggtcg gtgaatttgt atgcggatca ttgaatgatt acgatacggt
     1981 aatgcgattt ggtgccgaca aagatttgct gaccatagaa attgagcacg tcaatgtgga
     2041 tgcgttggag catctcgaaa aacagggtaa aaaagtatat ccccagccgc atgttctccg
     2101 aatgattcag gacaagggtg aacagaaaaa attctatcag caaaacaaca ttcccacggc
     2161 cccattttat ctggtagaga accgtgcagg aattgccttg catggggccg aatttccatt
     2221 ttttcagaaa ctgcgcaaag gtggttatga tggaaaaggt gtaacccgac tggatgatgt
     2281 gaatgaactc tccaaagcat tcgatgagcc cagtgttctt gaaaaactgg ttccattcaa
     2341 aaaagagatt tcggttattg tttcacggaa cgaaaatgga gaaatcgcac attttccggt
     2401 tgtggagtgt gaatttaacc cgattgccaa tctggtagaa ttcctttttt ctccttcaga
     2461 tatttcttca aaaactgaac acgatgcgat tcgcattgct cgtgaaattg cagaatgttc
     2521 tggcattgtt ggtttacttg ccgtggaaat gtttgtattg gatgatgata ccgttcttgt
     2581 aaatgaaata gcacctcgtc cgcataatag tggtcaccac accatcgaag ccaatatcag
     2641 ttcgcagtac gaacaacatt tacgtgccat ccttaatctt ccacccggat caacggatat
     2701 agttcgtcct gccataatgg tcaatcttct gggtgaagat gggtacaccg gagatgcagt
     2761 ttacaaaggc ctggaagaca ttcttcgcat tccgggagtg tttgtgcatc tgtacggtaa
     2821 aaagaccacc aaacccttcc gcaaaatggg acatgtaacc atacttgctg ataacataag
     2881 cgctgcccga tcgctggcgc gtaaagttca atccacctta aaaataatag cctgaattat
     2941 gagtgtacat gtaggaatca tcatgggaag tcagagtgat cttccggtaa tgcaggaagc
     3001 cgctgaaatg ctgaaagaat tcggaattca gttcgagatg actatcgtat cggcacatcg
     3061 cacacctgag cgtatgtttg attacgccaa aactgcagcg cagcgtggat taaaagtaat
     3121 tattgccgga gcaggtggtg cagcgcattt accggggatg acagcctcat taacagcatt
     3181 gcctgtaatt ggtgtcccaa ttaaatcctc caattctatc gacggctggg attcggtgct
     3241 ttccattctg caaatgccgg gcggggttcc cgttgcaact gttgccttga atggcgcaaa
     3301 aaatgcagga attcttgcag cgcaaattgt tggttcgggt gatgctgctg tgttgaaaaa
     3361 aattgaggct tataaagaag gactgaaaag caaagtactt gaaatggcaa aaaaactgga
     3421 gggatagaat ttaatttcca attatccgat ttccaattat ccgattactg attcattcta
     3481 ctttttatat tctgattccg atcatggtta cgtcatcgat ctgttcgagg tcgcctttcc
     3541 attgaaaaaa ctcctggttt aatttttcgc cgagttcgtt catggataaa tgcagatttg
     3601 ctgaaattga tttttgtaca ttcttgtact tcattttttt tccaccagga ccaccaaact
     3661 gatctgcaaa tccgtcggta aataaaacaa ttatatcgtt ttttctgatt tcaatctgat
     3721 gcgataaaaa cgattttctt ccctgaaatt tcccaatggg ttgtttatcc ggttttactt
     3781 ctatgatctc attctcccgg aaaatccaga ttccgttatt ggcacctgcc cattttaaaa
     3841 ttgaatctcc cttacgccac gagcacaatg aaatatccat tccatcccac acatcgcctt
     3901 gatttttaga aaaggtagct tcaactaatc gggccgtttc atccagtaca attcccggat
     3961 cggtttctcc aagttccttt acaacacgat tgagcgcatt cgcacataca acagaaacca
     4021 ttgcaccagg cacaccatgt ccggtacaat ctgctgctgc aaacagaacc tcctcttctt
     4081 tggtctccaa aaaatagaaa tcacctgcca cgatgtcttt aggcaaatac aatacaaacg
     4141 catcacccaa cagtcgggaa aaatcattta caggaggaag aattgcttgc tgcaatcgct
     4201 ttgcatagtt tatggaatca agaatctcac gattcttttc atcaaccagc agtttttgtc
     4261 gttctatttc cttcttctgt tgctgcgaaa ctttaaaacg gttgtaaaac atccaggcag
     4321 aaagtgcaat gatcacaaga aggacaacaa cgcctgacaa tattaagcgc tgtcgtttac
     4381 tttcttccgc gtgtaggagc tgctgggctt cctgctcttt tttaaattcc gtttcccgtt
     4441 ttccaaaatt catcagcatt tcctgtcgga gcgcattatc gcgatttttc tgattctgca
     4501 atgaatcgcg catggtaata tgcattttgt aaaaaggaaa tgcctttgca ggatcccctt
     4561 tcttttcata aatcatatac atgatcttcg aggtgcgcga cataatttcg ggataaccca
     4621 attcaaccgc cttcttatag gccttttcgc ccaattcaat tgcctttgaa aattctcctc
     4681 tgtcgcggta cacttctgca atgttatcgt aggcataaac aataccggca gcatccccca
     4741 actcctctcg taattgcaat cccttctcaa aatactcaat ggctctgtcg tgatcgccca
     4801 aatcaagata gactgttccc atgttggcca gtgaatacga catgcttcgc ttatccccgt
     4861 tttgttcagc ataggaatac gatcgggctg catgttctat agcctgctga aaggctttca
     4921 ttttgcaata gaggtacgac aaattattgt gcacatagga taatccaagg ggatcattga
     4981 tttcttccaa caaggcaagg caggcaaggt aagactccag cgcacgttca tcttctccca
     5041 tctcttcctg tacgctggca atattcagat agcagtaagc agaattcttc aactcaccgg
     5101 ccgactccag caaatagagg cttcggtata aataattaag tgttgtaccc aattcgccca
     5161 gctgataata gaccaatccg atattgttta cacactctgc aactcccacg cgcgattgca
     5221 ggtattcgaa taaaaaaagc gccttctcgt attcgagttt tgcatcatga atttgtccgg
     5281 cgttcttata atttaaaccc agacgaagat aaacacgtgc taattcttcc atttcatgcg
     5341 gctcgagaaa agcaacaagt gaacgatgat gataattgat tgagcgctta tatgcagcaa
     5401 ctgcagaatc tgaggaggta tcctcccact tttttcccaa tagaaattca tgccgggctt
     5461 taagtgaatc cgttaatgat ttcacattta cagcccgcat cgaatcaagt tcagtttgtg
     5521 gaacgcaaaa tgcgcgatta cctgaaataa tcacaaacac aaataaaaga caatagtcca
     5581 gcttcctcat accaggcgaa ttccgatcat actcacatca tcaatttgtt caatttcgcc
     5641 cttccattcc ctataggcag caaccagttc atctttcaat tgcggcatgc tgagggattt
     5701 tcgttgactg ataaactcct gcaaagtacg gtatttaaat tttttaccgc gtggtccgcc
     5761 aaactgatca gcgtaaccat ctgaaaaaag aaaaacagca tcaccctttt tcaatgaaag
     5821 tgtatgtgta acaaacggat gcgcttcgta tgaaaatccg ataggttgtt tatctgcggc
     5881 aatttcattc attgtatcgt tctctaaata ccaaagcgga ttatttgcac ctgcccattg
     5941 cagtgtaaac tcattctcct tgcgttctat ggtgcatagg gaaatatcca tcccatcctt
     6001 tacctcctga tcgctacgcc tgaaggtatc cattaccaat gctcgtgtcc gatcgagaat
     6061 ttttccggga tctgtttcac cgaattcttt taccacccga tttaatgcat tggaacaaac
     6121 aacacttacc atagcaccag gtactccatg tccggtacaa tcggcaactg caataataat
     6181 tttgttcttt tcctgctcca gaaaataaaa atcgcctgct acaatatcct tgggaaaata
     6241 gatcacgaaa ttttcaggca atagggaaga aatatactca gccggaggaa gaatagcctc
     6301 ctgcaagcgt cgcgcatagg tgatggaatc gagaatttcc ttgttctttt cctgcaaaag
     6361 ttctttttgt tcttctgcct ggatcttctg ctgttcgatg atacggcgtt gttttcttgt
     6421 ctgctgcaat gatctaaata taaaaaacac cgagacaaga ctaaggatga gtccgatgat
     6481 aacagagaat atgatgattc gctggcgacg gttttcgcct tctgcttttt ctgcttctgc
     6541 tgttaattgt gcttctcgtg ctttggcttc attggactcc agcacttttt gtttgttcag
     6601 caactcaagc tgattttgtt tggcttttat tttctcctga ttagcagcta gctcaatctc
     6661 tttgcgtgcc ctttcggaat tgagcagcga aatttgttgt tgctgccgga tagcctcgat
     6721 ctgctgaatt ttttgttcgc gggataacaa cataatctca cgttgctttt tatccagatc
     6781 atacttcgat tgcatttctg ttgcattctt gctcaatgat gctttaaaaa ctttttcgct
     6841 cagaatattc gactcaatca aatgccaata taccgaatcg gaattccctg ataaatactc
     6901 tgctttggca agagaacgga gtacttccat gtgtatatca tcaaaattac tgttacgcac
     6961 aagcgcatac gcttcctgca atgccgccat tgcctgggga atggaatcaa tttcagtgta
     7021 gagaattccg agattcatta atgattgaat caacctgtat ttctctcctc cctcacgacg
     7081 aagggcaact gtccatttgt acgaatcaat cgcctttttc cattttccct gtcgtttgta
     7141 aaccactccc aggttggtct cgataggaat aatcagactt ttatcgctac cgttttgaat
     7201 tgcgagatca cgcgccttct ccatgtaata ggcagcagaa tcattctgaa aagtatagag
     7261 atacacacct ccgatatttt gcaggcaacg aatctcacct tttttgtcac ccaatttaat
     7321 tttcagttcc agtgccatat aataatagcg aatggactca tcataactcc tcattgcatt
     7381 gagcgtattc ccaatggaga tcatcatggt tgaccggcgt ttatcatctt tcagcgaatc
     7441 ggctaatgcg agtgctttgt aatgatagcg aagcgagctc tcataatcgc ctttgatatt
     7501 gtaggcattg cctaaatacg aataccctgc agcgcgattg acctgcgaaa cctgcgagtt
     7561 ctgatcgaag tatttgggat cagtatgaat tgcgaaagat tgctgaacat actcgatggt
     7621 tttgtccgga tcgatataaa gatattccca acccaactca tcaagaatat aaaccctggt
     7681 tatgggatca gttgttttgt tcaactgttt ttgcagcgaa tcgatctttg cgttttgcgc
     7741 ataagctgtt cctgccggaa gtgcgaacac atacaacagg cgcagtgaaa acaggaaaac
     7801 ccgattcatc cggagaacac acataggcaa gtattcctgt gaatatacaa aggagcaagc
     7861 ataaatccga taagcaaaat aaaaaaggcc ctcattaaga gagcctttca attacatatt
     7921 gcgtcggtac tgaccaccta cctcaaataa ggcatgggtg atctgaccca gcgagcagta
     7981 tttggtagct tccattagtc cttcgaacaa attacggttc agaatagcag cctgttgcaa
     8041 ttcggaaaga tgttccttgc tttctgtctc gtacttctta tgcaggaaac gaatggtatc
     8101 gatttgttgt tgcttttctt cttcagtagc gcggatgacc tctgccggaa taatggtggg
     8161 tgagcctttg gaagacaaga aggtattcac accaatgatc gggaattcgc cggtgtgttt
     8221 gagtgtttcg tagtacagcg attcttcctg tattttactc cgttggtaca tggtttccat
     8281 agcgcccaat actccaccac gttcggtgat acgatcgaat tcggtgagca ctgcttcttc
     8341 cacaagatcg gtcatctcct caatgataaa tgccccctga atcggattct catttttcgc
     8401 aagtcccaac tcccggttaa tgatgagctg aatagccata gcgcgacgaa cagattcttc
     8461 tgtaggcgtt gttatagcct catcatacgc attggtatga agtgaattgc aattgtcgta
     8521 aatcgcatac aatgcctgaa gcgttgtacg aatatcattg aagtcaatct cctgcgcatg
     8581 caacgaacga ccggatgtct ggatatggta tttcaacatc tgcgaacgtg catcagcgcc
     8641 gtatttatct ttcattgctt tcgcccatat gcgacgagca acacgtccga tcactgcgta
     8701 ttccggatca ataccgttgg agaaaaagaa ggagaggttc ggaccaaatt cgttgatgtc
     8761 cattcccctg ctcaggtaat actccacata ggtaaatccg tttgccagcg taaatgcaag
     8821 ttgtgtgatt ggatttgctc ccgcttctgc aatatgatag cctgaaatag agaccgaata
     8881 aaaatttctt acttttttct gtatgaaata ctcctgcaca tcacccatta aacgaagagc
     8941 gaattcagta gagaagatac aagtattctg cgcctgatct tccttcagga tatcggcctg
     9001 tacagtaccg cgcacctgca tgagcgtttc cgttttgatt tttgcataca catctgcagg
     9061 aagaaccata tctccggtaa caccaagtaa catcaatccc aatccgtcat tcccctctgg
     9121 aagtggcgca ttgtatttgg gacgtggaac accgcgttgc tggtaaatgc tgtcgatttt
     9181 tttgctgatc tcagcttcga gtccgtttgc cttaatgtat ttttcgcatt gctgatcgat
     9241 tgcagcattc ataaaaaacc caagcagcat cggagcagga ccgttgatgg tcatggaaac
     9301 ggaagtcttg ggatcggata aatcgaaacc ggaatacaat tttttagcgt catccagaca
     9361 acaaatggaa acaccggagt ttccaatttt tccatagata tcgggacgaa ggtcaggatc
     9421 gtttccgtag agcgtaactg aatcgaatgc cgtagacaag cgttttgccg gcattcccag
     9481 cgaaacgtaa tggaagcgct tgttcgtacg ttccggacaa ccttctccgg cgaacattcg
     9541 tgttggatct tccccttcgc gtttgaaagg ataaattccg gccgtatagg ggaattcacc
     9601 cggaacattt tcgcggagca cccaacgcaa tacatcaccc catgatttgt aacgtggcgt
     9661 tgctactttg ggaacctgcg tgtgtgaaag tgattcggta tgcgttttta ttttgatctg
     9721 cttgtcgcgc acctggaaaa cataaaactc atccttgtat cgcttcacct tatccggcca
     9781 ttgctgaata agttcccaat tgtgcggatc aagatcgagt ttaattttat cgaactgcgc
     9841 acgtaatgtt tttacgatat ctgcatcttt cttttcatca aaagttttaa tggcagtatc
     9901 aattccgtac agacgatctg caatttccac ctgtttgatt acccaattgt cgtatttgcg
     9961 attattctca gagatctcag aaaggtaacg ggtacgagca ggtggaatca caaaaatctt
    10021 ctcactcatt tcccgggtga tttcaaatcc ggattttaag ggagcatttg ttttctccac
    10081 taccttgtcc ataatcgcc
//
LOCUS       DAOMYG010000049        10052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2231_length_10052_cov_9.790737, whole genome shotgun sequence.
ACCESSION   DAOMYG010000049 DAOMYG010000000
VERSION     DAOMYG010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10052
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2231_length_10052_cov_9.790737"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PK610_02690"
     CDS             <1..439
                     /locus_tag="PK610_02690"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRJ34861.1"
                     /translation="ISVLTETGDQILEFISSIPNEKADFKYETDKWSVKEVLIHIIDT
                     ERIYSYRALRFARNDKTELPGFDENHFGLHCNAAQRTLKDLLEEFSIVRKSTIALYQS
                     FDQENLLKSGIANEKRMTVRAIGHIMAGHALHHCNIIRERYLN"
     gene            459..3923
                     /locus_tag="PK610_02695"
     CDS             459..3923
                     /locus_tag="PK610_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRJ34862.1"
                     /translation="MKLIVLSAFVFLCNLAEAQVELITQTRQSGASTWISYSPSGNFI
                     ASASTNDHSIRVWDTRSGKIIGNLYGHDHPISVIYFDTDGRHLFSADKKGILLSWNLE
                     NWKQSDSLSLGDDVHAISLWKDHLIAGTVKGQIHLIRKKGLQKNDTFLSGKESVELLV
                     VDEKSDVLFAHTLTKGLQIWDLNSHKKTAQFPTEKDTPSAMYTTGNGKLLIVYSSGKI
                     SLWDTGQKKNLLSTNSPQGVTASDFNMKTSVLAVCGKDHKVRLYKTDNLQVEAREITA
                     GEGHGENTIRSLAFSPDGSTIATTGFKTERGFFSGASKSQNTIRVWSVSTGELYSELK
                     GSVRPVIAFGFFPELNRIAILHEGQELSFWDFDLGEKTGTFTLPEAKREYFPKMKHAA
                     DNAINNAGSAVNTAQNVLSGNVNLNAKKAAKSAGKATQRNLDKLVENDPVILFSALGK
                     YMITQLPKDEIRIYSVNNGAPQYQHYLKHDLHKLNVIAISPDETQVACGGIGNEFLTI
                     CDISNGNLIKSIRLPLPSGTENISELRDICYSNDGKLLAVVMNTGKLFVLDATSHSVL
                     FENLMPVTLSVFHGGLVNFTRDSKYLLYNGTDGLRKVSVGSFLDEKAKTFHVKGKVLP
                     MNSSQNFALSREKTGMNVYDLVYNTEFFIPSNSREVTCVGVSPGGRIALSFVSGEIRL
                     YEPTKGEHIATMVTEGENGIIKTRDNYYKVNKEGYDLVTFRVGSKAYPFEQFDLYFNQ
                     PARVLEALGSGNKSLIQLYQTTREKRLKRMGAEKAYFAIEELPVTEIRNRADIPALTN
                     ENAITLNIKATDERSGISGFQFWINNVPVFGPRILKTKNRKEADTTIQIPLINGINEI
                     RCAAFNGKGNESLNEAIRIEARYDVPVDLYLVSIGVSNYKDKKFNLQYAAKDARDIAA
                     LFNQNNVFRKVESKILTDAEVTKKNVLSLKDFLSKAKLNDVVMVFIAGHGVLDKQFNY
                     YFAPNLMDFNHPEREGISYESLETLVAAVKSIRKILIMDTCHSGEVDKDDVQSTVTNA
                     VETGDVQFRNAGENIDFKDAQQAGASKMMRELFADLRKGTGITAISSAGGTELAMESS
                     SFKNGLFTFCLLTGLKNKSADLNGDQQITSQELQRYLVKEVAEKSQGKQVPTARVDNF
                     MLDYLLWK"
     gene            4043..5566
                     /gene="purH"
                     /locus_tag="PK610_02700"
     CDS             4043..5566
                     /gene="purH"
                     /locus_tag="PK610_02700"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRJ34863.1"
                     /translation="MSEKSIKSALISVYYKDGLEAIVQELNTLGVTIYSTGGTEEFIR
                     KMGIDVVPVEDLTSYPSILGGRVKTLHPKVFGGILGRRAHSGDIEQLEQYEIPEIDLV
                     IVDLYPFEETVKSGAAEADIIEKIDIGGISLIRAAAKNFQDVVIVPSRDLYAEFLEIL
                     KSKKGKTSLDDRKKFMAYAFHISSHYDTAIFNYFNTGFDFLKISEQNSQVLRYGENPH
                     QKGTFHGDLNALFTQLNGKELSYNNLLDVDAAVNLMTDLDKGSPAFAILKHNNACGVA
                     IRSSLFDAWTAALAGDPVSAFGGILISNTEIDFATASEIDRLFFEVLIAPSYSKDALE
                     LLKGKKNRILLIQNKVELPKRQVRTALNGFLVQDKDSVVDRRSNYKTATKVAPTEQEL
                     SDLEFAGVLVKHTKSNTIVLAKNNTLLASGTGQTSRVDALRQAIAKAKSFGFELKGAV
                     MASDAFFPFPDCVEIAHGEGIHAVVQPGGSIKDQESIDYCDAQGMAMVITGTRHFRH"
     gene            5620..6645
                     /locus_tag="PK610_02705"
     CDS             5620..6645
                     /locus_tag="PK610_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRJ34864.1"
                     /translation="MGLFDFFTQEIAIDLGTANTLIIHNDKVVVDEPSIVAVDRTTNK
                     IIGVGRKAQLMHGKTHENIKTIRPLKDGVIADFHAAEHMIREMIKMLHPRKRLFNPSL
                     RMVICIPSGITEVEKRAVRDSAEHAGAKEVYLIHEPMAAAIGIGIDVEEPMGNMIIDI
                     GGGTSEIAVIALGGIVCDKSIRVAGDEFTSDIEEYMRRQHNILVGERTAERIKIEVGA
                     ALPELENGPEDYAVSGRDLMTGIPKEITVSYSEIAHALDKSISKVEEAILSALEMTPP
                     ELSADIYKTGIYLAGGGALLRGLDKRISMRTKLPVHIADDPLRAVARGTGIALKNIDR
                     FQFLIRD"
     gene            6658..7497
                     /gene="mreC"
                     /locus_tag="PK610_02710"
     CDS             6658..7497
                     /gene="mreC"
                     /locus_tag="PK610_02710"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRJ34865.1"
                     /translation="MRNLRLFIIRNYFFFLFLFFQVTALTMLVSSNNYQESTFMNSSL
                     VFVGFLMEKKTEFSEYFSLKDANRNLLDENVRLKNMLMQIFAADSTKTIFQKDSTGNT
                     QYTFRGAKVINNTVYAQDNFLTIDKGSRDGIAPDMGVTNGISIVGFVKDVSEHYATVV
                     SVLNKNFVLSVKLKKTNDHGLIKWEGQDPQRVMLNGITVDAPAKTGDTIVTRGASGRF
                     PEGVLVGIVEEVFQKPGAMHHSIIVRLSTPFNSVYHVYVVENHRMKEQLELEELLKGE
                     KQP"
     gene            7494..8009
                     /gene="mreD"
                     /locus_tag="PK610_02715"
     CDS             7494..8009
                     /gene="mreD"
                     /locus_tag="PK610_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRJ34866.1"
                     /translation="MIRDYSITILRGIFLLLLQGLVLNNLPLGTYFQPYLYVMFIILL
                     PFETPNWMILPLSFFYGLILDSFTNTYGLHASACTFLAWNRPQFLRLFAPRDGYDAAT
                     LPGIQAYGFNWFISYAGSLILMHHIVFFLLEAFRFEGIGHTLLRCLGSASFTLFLAII
                     AQYLFLRPQTR"
     gene            8010..9875
                     /gene="mrdA"
                     /locus_tag="PK610_02720"
     CDS             8010..9875
                     /gene="mrdA"
                     /locus_tag="PK610_02720"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRJ34867.1"
                     /translation="MRNYENRQFVLLLIYITVAVIFIIRLLFMQVLTDRWTERAAQIS
                     EYKLYTYAPRGIIYDRYGNKLVENKTFYDMVVTPAKMRPMDTIAFCRLVGITKEDFIK
                     KITIAKNYSQHIPSEFEKQISADNFELISEELYKYPGFFAQERTMRGYRKEIGAHVFG
                     YISEVSPEDIEKNPYYRPRDYIGKKGLEQSYEEILRGERGVRYLLKDAIGKESGSYEG
                     GKYDTMAVQGKNIYCTIDADLQEYGEKLMQNKKGSIVCIEPETGEILALVSSPTYNPS
                     LMTGREKGKNYYGLLNNDSLKPLYNRAIQAMYPPGSIFKMAQALIGLQEGVITINTGF
                     PCDKSLVGCHNHPAASDIKRAVQFSCNPYFFRATQRIIQQGKSRSIFKDSELGLRKWT
                     EYMHEFGFGVDLGVDIPSLKKGFIPTVEFYDKYYGKGRWAFSTIYSISIGQGEVLVVP
                     LQMANFTAHIANRGYYYTPHFVKGIDSIGNIPEKFREKHQMRINREYYDPLVEGMRAV
                     VEEPGGTASSARLKDITVCGKTGTAQNPHGEDHSVFIAFAPMDKPKIAISVYVENAGF
                     GGVWAAPIASLMIEKYLKGSVTDTLKEQRMMNAVILDVKKDKATTNAKSRKRLGI"
     gene            9872..>10052
                     /locus_tag="PK610_02725"
     CDS             9872..>10052
                     /locus_tag="PK610_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRJ34868.1"
                     /translation="MRTDNPVRKIDRFLLITYLIMVTIGYFNIYSSAYNPEHPSHFDF
                     SMEYGKQIIWIGVSLL"
BASE COUNT         3048 a         2136 c         2227 g         2641 t
ORIGIN      
        1 gattagcgta ttgacggaaa caggagatca aatccttgaa tttatttcct ccattcccaa
       61 cgaaaaagca gattttaaat atgaaacgga taaatggtcg gtaaaggaag tcctgattca
      121 cattatcgat accgaacgca tttacagtta ccgtgcatta cgctttgcac gcaacgacaa
      181 aaccgagcta ccgggctttg atgaaaatca ttttggtctt cattgcaatg ctgcacaacg
      241 cacactgaag gatcttttgg aagagtttag catcgttaga aaaagtacga ttgcacttta
      301 ccaaagtttt gatcaggaaa atttgctgaa gagcggaatt gcaaatgaaa agcgaatgac
      361 ggttagagca attggtcaca taatggccgg ccatgccctt caccattgca atattatccg
      421 agaaaggtac ctcaactgaa tctaccagcc ccactattat gaaattgatc gttctttctg
      481 catttgtttt cctgtgtaat ctcgctgaag cgcaggttga actcatcacc caaacccgac
      541 agtccggagc gagtacctgg atcagttatt ctccttccgg taattttatt gcatcagcaa
      601 gcacaaatga tcattccatc cgagtatggg acacccgaag cggaaaaatc attggaaatc
      661 tctacggaca cgatcatcca atttctgtga tctacttcga taccgatgga cggcatttgt
      721 tcagtgcaga taaaaaaggg atactgctat cctggaatct tgaaaactgg aaacaaagtg
      781 acagcctctc tctcggtgat gacgttcatg ccatttcgct ttggaaagat catctgattg
      841 caggtaccgt taaaggacaa atacacctga ttcgaaaaaa aggattgcaa aaaaacgata
      901 ccttcctatc aggaaaagaa tcggttgaac ttttggttgt cgatgaaaaa agcgatgttc
      961 tgtttgcaca cacactcact aaaggtttgc agatctggga tctgaacagt cacaaaaaaa
     1021 ccgcacaatt tcctacggaa aaagacactc cttctgcaat gtacacaaca ggaaacggta
     1081 aacttctaat tgtgtattca agcggaaaga tcagtctgtg ggatacaggg caaaagaaaa
     1141 atctcctttc caccaattct ccacaaggag ttacagcaag cgatttcaac atgaaaactt
     1201 ctgtacttgc tgtttgcgga aaagaccata aggttaggct ttacaagacc gacaacctgc
     1261 aagttgaagc ccgtgaaatc actgcaggag aaggtcatgg cgaaaacacc attcgctcgc
     1321 tggcatttag tccagacggc agcaccattg caacaacagg atttaaaacg gaacgaggtt
     1381 tcttttcggg tgcatcaaaa agtcagaaca ccattcgcgt ctggtcggtt tccaccggag
     1441 aattgtattc ggaacttaaa ggaagcgtac gaccggttat tgctttcgga ttttttcctg
     1501 aactgaatcg tattgccata cttcatgaag gtcaggaact cagtttctgg gatttcgatc
     1561 tgggcgaaaa aacaggaaca ttcactttac cggaagccaa aagggaatat ttcccgaaaa
     1621 tgaaacatgc agccgataat gccattaata atgcaggatc ggcagtgaat accgcgcaaa
     1681 atgtattaag tggtaacgta aatctgaatg caaaaaaagc agccaaatcg gccggaaaag
     1741 caacccaacg taatctcgat aaattggttg agaacgatcc cgtaattcta ttttctgcct
     1801 tgggaaaata catgatcacc caattgccaa aagatgaaat caggatttac tcggtaaaca
     1861 atggtgcgcc acaataccag cactacctga aacatgatct acacaaactg aacgtcattg
     1921 ccatttcgcc ggatgaaaca caggttgcat gtggtggaat tggaaatgaa tttctaacca
     1981 tctgcgatat ttcaaacgga aacctgatca aatcgatccg acttcctctt ccctcaggta
     2041 ccgaaaacat ttctgaattg cgtgatatct gttacagcaa cgacggaaaa ctgcttgcgg
     2101 tggtcatgaa tacaggaaaa ctatttgttc tggatgcaac ttctcattcg gtgctattcg
     2161 aaaaccttat gccggttacg ctatctgtat ttcatggggg attagtgaat ttcacccgcg
     2221 attcgaaata cctgttgtac aatggaacag acggcttgcg caaagtaagt gtgggtagtt
     2281 tcctcgatga aaaagcaaag actttccatg taaaaggaaa agtcctgcca atgaacagct
     2341 cgcagaattt tgccctttcc cgagagaaaa ccggaatgaa cgtctatgat ctggtctaca
     2401 ataccgaatt cttcattcca tccaacagca gagaagtcac ctgtgtaggt gtgagtccgg
     2461 gcggacgcat tgcgctcagt ttcgtttcgg gtgaaattcg cctgtacgaa cccacaaaag
     2521 gagaacacat tgccacaatg gttaccgagg gagaaaacgg aatcatcaag acccgcgaca
     2581 actattacaa agtcaataag gaagggtacg atctggtgac tttccgtgtt ggatccaaag
     2641 cctatccctt cgagcaattt gatctctatt tcaatcagcc tgcacgcgtt cttgaggcat
     2701 tgggatcagg caacaaaagc ctgattcagc tctaccagac cacccgtgag aagcgactca
     2761 aacggatggg agcggaaaaa gcatatttcg caattgaaga actacctgta accgagatcc
     2821 gcaaccgcgc cgatatcccg gctctcacca acgaaaatgc aattacgctt aacatcaaag
     2881 ctacagatga acgctcaggg atcagcggat ttcaattttg gatcaacaat gtaccggtgt
     2941 tcggaccacg tatcctgaaa acgaaaaacc gcaaggaagc cgataccacc attcagattc
     3001 ctctcatcaa tggaatcaac gagatccgct gtgctgcatt taatggcaaa ggaaacgaaa
     3061 gcctgaatga ggccatccgt atcgaagccc gatatgatgt tccggtcgat ctgtacctcg
     3121 tttccattgg tgtttccaat tacaaggaca aaaaattcaa tcttcagtat gccgccaaag
     3181 acgcccgcga tattgcagcc ttgtttaacc aaaacaatgt cttccgcaaa gtggaaagca
     3241 agatccttac cgacgcggaa gtcacgaaaa aaaatgttct ttccctgaag gatttccttt
     3301 ccaaagcaaa attgaacgat gtagtgatgg ttttcattgc ggggcacggg gtgctcgaca
     3361 agcaattcaa ctactatttc gctccgaacc tgatggattt caaccacccc gaacgtgaag
     3421 gtatcagcta tgaatcgctc gaaacactgg tagctgcggt taaatccatt cggaaaattc
     3481 ttatcatgga cacctgccac agtggggaag tggacaaaga cgatgttcaa tcaacagtta
     3541 ccaatgcagt tgagacgggt gatgtacaat ttcgcaatgc aggggaaaac atcgacttca
     3601 aggatgcaca acaagccggg gcttcaaaaa tgatgaggga actctttgcc gacctccgaa
     3661 agggaacagg gatcacggcc atttcaagtg caggcggaac agaacttgct atggaatctt
     3721 cttcctttaa aaacggcttg tttacgtttt gcctcttaac gggactgaaa aacaaatctg
     3781 ccgatctgaa tggagatcaa caaatcacca gtcaggaact ccaacgctac cttgtaaagg
     3841 aagtcgctga aaaatcacag ggaaaacagg taccaacagc gcgggtcgat aattttatgc
     3901 tcgactattt gctctggaaa tagctctttg cttcacttac tcgtcacctt tcaacattcc
     3961 cgcttcattt ttcaacactt aatggaagag cctctttcga tgagttgatt cttgctactt
     4021 tcgtcgctta caaatcaaaa tcatgtcaga aaaatccatt aaatccgccc ttatttctgt
     4081 ttattacaag gacggactcg aggctattgt gcaagagctc aacactttgg gtgtaactat
     4141 ttactcaacg ggaggtaccg aagaattcat ccgaaaaatg ggaattgatg ttgttccggt
     4201 tgaggacctc acctcttatc cgagcattct tggggggcgt gtaaaaacgc ttcatcctaa
     4261 agtgtttgga gggatcctcg gaaggagagc tcacagcggg gatattgagc agctcgagca
     4321 atatgaaatt cctgagatcg atttggtcat tgttgacctc tacccttttg aggaaactgt
     4381 aaaatcaggt gctgcagaag cagacatcat tgagaaaata gatattggcg gaatttcact
     4441 tattcgtgct gctgcaaaga attttcagga tgtagtgatt gtcccatcac gtgatcttta
     4501 tgctgagttt ctggaaattc ttaaaagcaa aaaaggcaaa acgagtcttg atgacagaaa
     4561 gaaattcatg gcctacgcct ttcatatttc ttcgcattat gataccgcaa tcttcaatta
     4621 tttcaatacc ggtttcgact tccttaaaat cagcgagcag aacagccagg tcctcagata
     4681 cggtgaaaat ccacaccaaa aaggaacctt ccatggcgat ttgaatgcac tttttaccca
     4741 acttaacggt aaggagcttt cctataacaa cctcctcgat gtggatgcag cagtaaacct
     4801 gatgaccgac ctcgataaag gttcacctgc atttgccatt ctcaaacaca ataacgcctg
     4861 cggtgttgcc attcgcagca gtctctttga tgcctggacg gcagcattgg ccggcgatcc
     4921 agtttcagca tttgggggta tactaatcag caatactgaa atcgattttg caaccgccag
     4981 cgagatcgac cgactcttct ttgaagtatt gatcgcaccc tcctattcga aagatgcatt
     5041 ggaacttctt aaaggaaaaa agaaccgtat tctactgatt cagaacaagg tagaattacc
     5101 aaaacgtcag gtaagaacag cgctgaatgg attcctggtt caggacaagg acagcgttgt
     5161 ggaccgccgt tcgaattata aaaccgcaac taaagtcgcc ccaaccgagc aggaactgag
     5221 tgatctcgag tttgccggtg tattggtaaa acatacgaaa tccaacacca ttgtacttgc
     5281 aaaaaacaac actttacttg ctagcggtac cggtcaaacc agccgagtag atgctcttcg
     5341 tcaggccatt gccaaagcga aaagtttcgg gtttgagctg aaaggtgcag taatggccag
     5401 tgatgcattt ttcccatttc cagattgtgt agagattgca cacggagagg gaattcatgc
     5461 tgtagtacaa ccaggcggat cgatcaaaga tcaggagtcg attgattatt gcgatgcaca
     5521 ggggatggca atggtgatta caggaacgcg acatttccgt cactaattgc ggtattaaaa
     5581 aaaacttatc tttataagaa ataaaaccgg aggagcagca tgggactttt tgattttttc
     5641 acacaggaga tcgccatcga cttaggcaca gcgaacacac tgattattca caatgataaa
     5701 gttgttgtgg atgaaccatc gattgttgcc gtagatcgaa ctacaaataa aattatcggt
     5761 gttggacgca aagcacagtt gatgcatgga aagacacacg aaaacattaa aacaattcgt
     5821 ccgcttaaag acggagtaat tgccgacttc catgcagcgg agcacatgat cagagaaatg
     5881 atcaagatgc tgcatccgcg taagcgatta ttcaatccat ccttgcgcat ggtgatctgt
     5941 attccatcag gtattactga agtggaaaaa agagcggtac gcgacagtgc agaacatgca
     6001 ggtgcaaaag aagtatatct tattcatgaa ccaatggcag cagcaatcgg tatcggtatc
     6061 gatgtggaag agccaatggg gaacatgatc attgatatag gaggaggaac ttctgagatt
     6121 gcggttatag cgcttggtgg aattgtttgt gataaatcca ttcgtgttgc gggagatgag
     6181 tttacaagcg acattgaaga atacatgcgt cgccaacata acattttggt tggagagcgt
     6241 accgctgaac gcataaaaat tgaagttgga gcggcattac ctgaattgga aaatggtccg
     6301 gaagattacg cagtaagtgg tcgtgacctc atgaccggta ttccgaaaga gattactgtt
     6361 tcgtacagtg agattgcgca tgctttggac aaatccatct ctaaagttga ggaagcgatt
     6421 ttaagtgcgc ttgaaatgac acctcctgaa ctctctgccg atatttacaa aaccggtatt
     6481 tatcttgccg ggggtggggc attgcttcgt ggtcttgaca aacgtatttc aatgcgcacc
     6541 aagttaccgg tgcatattgc cgacgatcca cttcgtgcag ttgcgcgtgg tacaggaatc
     6601 gcgctgaaaa acatcgaccg cttccaattt cttattcgcg attaatcctg ctgctgaatg
     6661 aggaacctga ggctgttcat catcaggaat tatttctttt tccttttcct gttttttcag
     6721 gttactgcac ttactatgtt ggtcagcagc aacaattacc aggaaagtac cttcatgaat
     6781 tcgtctttgg tttttgttgg cttccttatg gaaaagaaaa cagagttctc ggaatacttt
     6841 agtctgaaag acgccaaccg aaatcttctt gatgaaaatg tccgtttaaa aaacatgctg
     6901 atgcagatct ttgctgctga tagcaccaag accatttttc agaaagacag cacaggaaat
     6961 acccaataca ctttccgggg ggcgaaagtc attaataaca ctgtttacgc acaagataat
     7021 tttctgacaa ttgataaagg cagcagggat ggaattgctc cggacatggg tgtaaccaac
     7081 ggcatttcca ttgttggttt tgtaaaagat gtatcggagc attacgcaac tgtggtatct
     7141 gtactcaata aaaatttcgt gttaagcgta aaactgaaaa agaccaatga tcacggattg
     7201 atcaaatggg aaggtcagga tccacagcgt gtaatgctca acggcattac agttgacgct
     7261 cctgcaaaaa ccggcgacac gattgtaacg cgcggcgcca gtggacgatt tcctgaagga
     7321 gttttggttg ggatagttga agaagtattt cagaaaccgg gcgccatgca tcactctata
     7381 attgtgcgtt tatcaactcc ttttaattcg gtatatcacg tttatgttgt agaaaatcac
     7441 cggatgaagg agcaattgga actggaagag ttgctgaaag gagaaaagca accatgattc
     7501 gcgattacag cataactatt ttacgcggca tttttcttct gctattgcaa ggattggttt
     7561 tgaataatct tcctctagga acttattttc agccgtattt gtacgtgatg tttatcattc
     7621 ttcttccgtt tgaaacacct aactggatga tccttccact tagttttttc tatgggctta
     7681 ttttggattc atttacaaac acctatggac tacatgcatc agcatgtaca tttttagcat
     7741 ggaatcgtcc gcaatttctt cgcctgttcg ctccaagaga tggttatgat gctgccacat
     7801 tacccggcat tcaggcctat ggtttcaatt ggttcatctc ctatgcagga tcacttatcc
     7861 tgatgcatca tattgttttc ttcctgctcg aagcattccg ttttgaaggt atcggacaca
     7921 ctttacttcg ttgcctcgga tctgcaagtt ttacgttgtt ccttgcaatc attgcccagt
     7981 atttgtttct ccgacctcaa accaggtaaa tgagaaacta cgaaaatcgc cagttcgtcc
     8041 tcttgctgat ctatataacg gtcgcggtga ttttcattat ccgcttattg tttatgcaag
     8101 tcctgaccga ccgctggaca gaacgtgctg cacagatttc ggaatataaa ctttacacct
     8161 atgcgccgag gggaatcatt tacgacaggt atggaaataa gcttgtagaa aataaaacgt
     8221 tttacgacat ggttgttacg ccggcaaaaa tgcggccgat ggacaccatc gcattttgtc
     8281 gtttggtggg aataacgaaa gaagatttca ttaagaaaat aaccattgca aaaaattatt
     8341 cccagcatat tccatccgaa tttgaaaaac agatctcagc cgacaatttc gaattgattt
     8401 cggaggagtt gtataaatat cccggattct ttgctcagga gcgtacgatg cgtggttatc
     8461 gtaaagaaat tggagcgcat gtatttggtt acatcagtga agtcagtccg gaagacatcg
     8521 agaagaatcc ctattaccgt ccccgcgatt acatcggaaa aaagggcctg gagcaatcgt
     8581 atgaggagat tttgcgcgga gaacgagggg ttcgttattt attgaaagat gccattggta
     8641 aggaaagcgg aagctatgaa ggcggaaagt acgataccat ggcggttcag ggaaaaaaca
     8701 tttattgtac gatagatgca gatctgcagg aatacggaga gaagttgatg caaaacaaaa
     8761 aaggaagtat tgtttgcata gaaccggaaa ccggagaaat tctcgccttg gtttcatcgc
     8821 caacgtacaa tccatcttta atgaccggaa gggaaaaagg aaaaaattat tatggattat
     8881 tgaacaatga ctccttaaaa cccctttata accgagccat tcaggcgatg tatccaccag
     8941 gttccatctt taaaatggca caagcactaa ttggtttgca ggaaggagtg ataacgatca
     9001 acaccggatt cccctgcgat aaaagtctgg taggttgtca caaccatcct gcggcaagcg
     9061 acataaaacg agctgttcag ttttcatgta atccctattt cttccgtgcc acccaacgca
     9121 ttattcagca gggaaaatcg cggagtattt ttaaggactc ggaattagga ttgcgcaaat
     9181 ggaccgagta catgcatgaa ttcggttttg gagttgactt aggagttgat attccatccc
     9241 tgaagaaagg gttcattccc acagtagagt tttacgataa gtattacgga aaaggacgtt
     9301 gggcattttc aacgatctat tccatttcga ttggacaggg agaagttctg gtggtaccgc
     9361 ttcagatggc caactttact gcacacattg ccaacagagg ttattattat actccgcatt
     9421 tcgttaaggg tattgactcc attggaaata ttcctgaaaa attcagagag aaacaccaga
     9481 tgcgtattaa ccgtgaatat tatgatcctc tggttgaagg aatgcgagca gttgttgaag
     9541 aacccggtgg aacagcatcg agtgcacgtt taaaagatat tacggtttgc ggaaaaacag
     9601 gtaccgcaca aaatccgcat ggagaagacc attcggtatt cattgcattc gccccaatgg
     9661 acaaacccaa aattgccatt tcagtttacg ttgaaaatgc aggttttgga ggagtatggg
     9721 cagcaccgat tgcgagctta atgattgaaa aatacctgaa aggcagcgtt acagatacac
     9781 ttaaagaaca gcgcatgatg aatgcagtga ttctcgatgt gaaaaaagac aaagccacta
     9841 ccaacgcgaa aagcagaaaa cgtctgggga tatgagaacc gataatcccg ttagaaaaat
     9901 tgatcgcttt ttactgatca cctacctgat catggtaacg ataggttatt tcaatattta
     9961 ttcatcagcc tacaatccag aacatccatc gcatttcgat ttcagtatgg aatacggaaa
    10021 acagataatc tggattggag tatcgctctt ac
//
LOCUS       DAOMYG010000050         9982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2255_length_9982_cov_7.907626, whole genome shotgun sequence.
ACCESSION   DAOMYG010000050 DAOMYG010000000
VERSION     DAOMYG010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 9982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9982
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2255_length_9982_cov_7.907626"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="PK610_02730"
     CDS             complement(<1..1242)
                     /locus_tag="PK610_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRJ34869.1"
                     /translation="MQKLTAVEALASIPNRSRVFVQGSAASPTYLLNQMAAMADHWEQ
                     LELVSISLLGDVAPARPEFMHKFIFNSLFVSTPVRQTVNEGPSDYIPIFLSEIHKLFE
                     RGFLKPDVAIIQVSPPDQHGYCSLGVSVDIARSAVRAARIVIAQCNAKMPRTHGDGLI
                     HTSEIHYLVEAEQELPVVDYGSKITGLERTIGKHCASLIEDRSTIQAGIGTIPDAVLE
                     ALSGHKDLGVHTEMFSDGVMKLIEKGVVTNKFKKKYKGRTVTSFAIGTRKLYDFIDDN
                     PQFAFLEAGYVNDERTIRANPRVVAINSAIEIDITGQVCSDSIGKMQYSGVGGQMDFI
                     RGASFSEEGKPIIAIPSVTNKGISRIVPVLKEGAGVVTTRAHVHYVVTEYGVVNLFGK
                     NLQQRAEALCSIAHPDHRESID"
     gene            complement(1252..1803)
                     /locus_tag="PK610_02735"
     CDS             complement(1252..1803)
                     /locus_tag="PK610_02735"
                     /inference="COORDINATES: protein motif:HMM:NF021952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HRJ34870.1"
                     /translation="MKPQLFFTAFLFQDDVSAEIQSLKQELAERTNARHTLKILPHIT
                     LLPPATFTEEEIIIKKQLLEEWSRQIHVFEIKLKGFGHFRKDVVFIQPEIPQEIYSHQ
                     QELVKKMQSAFPGKFPEYSHPWHPHATLAFRDLNEESFQIAIEFLRGKKIQIQTTVHS
                     FAELKHQTIWVTETLFPLPAEKP"
     gene            complement(1800..3272)
                     /locus_tag="PK610_02740"
     CDS             complement(1800..3272)
                     /locus_tag="PK610_02740"
                     /inference="COORDINATES: protein motif:HMM:NF018239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRJ34871.1"
                     /translation="MAGKGKDKPENNSDLLLRIALLERENALLRKGIRSIHLNGETVK
                     VPEPFEDIFQRAQETVGKYFSGIQIEPTKATIEIEGQRYILVRASALSIDFFEKLKDL
                     YSDKGEKEAVEIGRNFLFDMAHVIGLQDAKAFHKVMNLKNPVEKLSAGPVHFAYTGWA
                     FVDISPESNPSPDDNFLLKYDHPFSFEADSWVNAGKTSDFPVCIMNAGYSSGWCEASF
                     GLPLTAVEVTCRAMGHDKCSFVMAPPHRIKDYLPGVSEIHDSNDVYSIPQFFERKQAE
                     EAIKSSLAEKEVLLKEIHHRVKNNLQIISSLLKLQSSYFEHPQLHKLILESQSRIKTM
                     AIVHEKLYQSNLGWVDLFEYLSTVAESAGESITPPGVEIHFDLVPPGEPIRLKIDQAI
                     PFGLIVNEIVTNSFKHAFTGKKKGEIGIHFSNGDEPGRMSLEIYDNGNGFPATQSDAY
                     SSFGLELIHMLADQLDAQIRIDQRKGTKYWFHFPYAVPEE"
     gene            complement(3319..5745)
                     /locus_tag="PK610_02745"
     CDS             complement(3319..5745)
                     /locus_tag="PK610_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRJ34872.1"
                     /translation="MSVAADYLLLIRRHSQLSKELLSPLAKRIFRFSIFNWSKFRSLR
                     WFIKPLSLLYSFFLSIALFVLAIELNFLWLFGTMPSISMVANPDLPTASEVYSADGVL
                     LGKFFQENREPIEFGDLNNNIINALVVTEDVRFYKHWGIDLKSIAGAVYSTARGDKRG
                     GSTLTQQLAKNLFKTRKRRSTGLLGYLPYVNTVIAKSKEWITAVKLEMFYTKEDILTM
                     YLNTVDFGHNTFGIKVAAHSYFGVYPKDLSVPQASLLVGLLKAPTYYSPLKNPERCLK
                     RRNTVIGQLLKYGKITTEEHDLYCSSPLGVEYREYSYSEGIAPYFRTAVAKSLSEWQK
                     ESGYNIFSDGLIIHTTLDSRMQAHAERAVAIQMKALQQRFKMHWSDRKPWQDKATAKN
                     KDGFLWDLVRQSDVYKKLKKKYPDDEKSLMAAMEEKKKMKIFTWEGEKEVQYSSLDSI
                     KHYIAFLHCGMITIDPANAHVKVYVGGVNYDYFKYDHVSVAMNQPGSTFKPFVYTTAI
                     EKGWGPCDKLPDVPVAIKYKENGENKVWTPHNADWSNSGQLVTLRHAMGRSINTVTAL
                     LTEKVGWENVAELAKRMGIKSPLKAVPSIGLGSNDVTLIELIGAYSVYMNKGIWNEPM
                     LVTHITDRKGNTIAEFKPESRRAISEETAWLMTYMLRGGFEEPGGTSQALWEFKFVHG
                     NEVGGKTGTSSNYADGWYIGLTKDYVTGVWVGADDNRIRFRTSETGEGARTALPVYGK
                     FMDLLYTDKKLLLSKGKFPEPGVTISKPHNCRTFASYSDSGIIAKDSVLISNPVLPEV
                     EGVPEENPEN"
     gene            5913..8471
                     /locus_tag="PK610_02750"
     CDS             5913..8471
                     /locus_tag="PK610_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRJ34873.1"
                     /translation="MTVILFYGCSQTRRLEPGQYLLTRNKIQFQGDDGKISQAEIEYL
                     LKQKPNRKIFKYPFHLFIHNLPNREKTERKSEAKKLECQKRNLELQVKLDTLNIEIER
                     NRKAIVGRKGRDSTRIANEIYRLEIKKIDLEDKMENCRAVLGHRMMYSIGEEPSVLDS
                     SRMETSVRNINLYLFKKGYFNNQVSSGVKYTGKKAEVIYTIKLGAPYKIRTINYQIKD
                     RRLSREIRSADPGTLVAVGNRLDYTILDKERDRITTHLRDKGYHFFSKEFIYFDIDTT
                     VGNLQVDLVMGVKNLIRTHPEIPDSLIEVPHQKYIIGNIFVNTDFDPSIANSTDKYRL
                     DLYDTLVGDKETQGIHFIYLRNKGISIKPKVLIDAIYIKRLYDYRAKEVEYTFKKLSS
                     LGVFRTINIQFEVGEEDPTGNYLNCYINLSRLPRQNISIESNGTNRGGNLGVAGNFTY
                     RNRNTFRGAETFRFSMSGGLEAQQALANEDQTIVGRTFNTLEFGPEIALYIPKFISPI
                     PFYKFDKAASPRTVFTASLNYQDRPDFVRTIQNVSYAYEWKGKKHTSFYLSPVKLSAV
                     KIRPDSSFTARLEALNDQFLLNSYRDHFIIGNQFRYEYNNQDPNSSKRNSLFFKVGLE
                     QSGNLMRLAYELAGMPHDSVGSIFGIQFAQFLKVDADFRFTQSFNKSSRVVYRVAGGV
                     GLPQKNFNQSLPFEKSFFVGGSNGLRGWKARTLGPGSYMGARVAFDKIGDIYLEGNVE
                     YRFDLFGFLKGALFADAGNIWNLRENPARPGSKFTPNFYQELALDGGLGLRLDLDFFI
                     IRFDFAIPLKEPGLMDGERWIFQDKTESNTWLNARGSGDYRPRPNLNLGIGYPF"
     gene            8534..9595
                     /locus_tag="PK610_02755"
     CDS             8534..9595
                     /locus_tag="PK610_02755"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008991340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HRJ34874.1"
                     /translation="MATNVTQLLGKDAESLLGHQCKTIDKSMLQLPGTNFIDNNFAGT
                     NRNPQVLRSLASIYNHGRLGGSGYVSILPVDQGIEHSAGASFAPSPIYFDPENIVKLA
                     VEGGCNAVASTYGVLASCSRKYAHKIPFIVKINHNEFLSYPNKYDQILFGSVDEAWNL
                     GAVAVGATIYFGSDESARQIVEIAQAFERAHELGMATILWCYLRNPGFKKDGVDYHTA
                     ADLTAQANHLGVTIQADIIKQKLPENNGGYNAINFGKTHKKVYSDLSSDHPIDLCRYQ
                     VANCYMGRIGLINSGGASTGSGESDLSEAVKTAVINKRAGGHGLISGRKAFQKPMNEG
                     VQLLNAIQDVYLDKSVSIA"
     gene            9611..>9982
                     /locus_tag="PK610_02760"
     CDS             9611..>9982
                     /locus_tag="PK610_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HRJ34875.1"
                     /translation="MSDSMGWFKRSKEGILTSTKDKKETPEGLWYKCPECGHVATTED
                     HADHMWVCEKCSYHEKISAEQYFLILFDDHEFTEFAANVISGDPLEFVDTKNYKDRIK
                     DTIAKSGLKDAIRCAYGTMEDQ"
BASE COUNT         2802 a         2255 c         2110 g         2815 t
ORIGIN      
        1 atcaatcgat tcccggtgat cgggatgagc aatgctgcat aaggcttctg ccctttgctg
       61 aagattttta ccaaaaagat taactactcc atattcggtt accacgtaat gaacatgtgc
      121 tcttgttgtt accactcctg ctccttcttt cagaacagga acaatgcgcg aaatgccttt
      181 gttggtgacc gatggaatgg caataatggg tttaccttct tcagaaaacg atgctccacg
      241 aatgaaatcc atttgtccac ccacaccgga atattgcatt ttaccaatgg aatcggagca
      301 aacctgtccg gtaatatcaa tttcaatagc actgtttatg gctaccaccc ttggattggc
      361 gcggatggtt cgttcgtcgt ttacataacc cgcttccaga aaggcaaact gaggattatc
      421 atcaataaaa tcgtacaatt tgcgtgttcc tattgcaaag gatgtaacgg ttctgccttt
      481 gtattttttt ttgaatttat tggtcaccac tcctttttca ataagtttca ttacaccgtc
      541 ggaaaacatt tcggtatgca ctccgaggtc tttatgtccg ctcaaagctt ccaaaacggc
      601 atccggaatg gtaccgatac cggcctgaat agtggaacgg tcttcgataa gggaggcaca
      661 atgtttcccg attgtccttt ccaatccggt tattttactt ccataatcca ccaccggaag
      721 ctcctgctct gcttctacca gataatgaat ttctgaggta tgaatcaatc catcaccatg
      781 tgtccttggc attttagcat tgcattgggc aatcactata cgggcagcac gcacagcaga
      841 acgggcaata tctacagata cacccaggga gcaatacccg tgttggtcag gcggactaac
      901 ctgtattatg gcaacatccg gtttcaggaa gccacgttcg aacagtttat gaatttcact
      961 gaggaaaatg ggaatataat cactcggacc ttcattaacg gtttgcctca cgggagtaga
     1021 aacaaacaat gaattaaaaa tgaatttatg cataaattcc ggtcgcgcag gagccacatc
     1081 gccaagcaga ctgatgctta caagctccag ttgttcccaa tgatcggcca ttgcagccat
     1141 ttggtttagc aggtaggtag gactggcagc actaccctgg acaaaaacgc gggagcgatt
     1201 cggtatggac gccagagctt cgacggcggt tagtttttgc atggtctaaa tttacggctt
     1261 ttcagcagga agcggaaaaa gggtttcggt cacccaaatt gtttgatgct tcaactctgc
     1321 aaacgagtga acagttgttt gaatttgtat ttttttacct ctcaaaaact caatggcaat
     1381 ttggaacgat tcctcgttta aatccctgaa agcaagtgtt gcatgcggat gccagggatg
     1441 agaatactca ggaaacttac cgggaaaagc gctttgcatt tttttgacca actcctgttg
     1501 atgggaataa atctcctgtg gaatttcagg ttgaataaaa acaacatcct tgcgaaaatg
     1561 gccaaatcct ttgagcttta tttcaaacac atgaatttga cgtgaccatt cttccagcag
     1621 ctgttttttg attatgattt cctcttctgt aaaagtggca ggaggaagta gtgtgatgtg
     1681 tggaagaatt tttaatgtat gtcgggcatt tgttcgttca gccagttctt gttttaggct
     1741 ttgaatttca gctgaaacat catcctgaaa aagaaaggca gtgaaaaaca attgtggttt
     1801 cattcctcgg gaactgcata gggaaaatgg aaccagtatt tagtgccttt ccgctgatct
     1861 attctgattt gggcatccaa ctgatcggca agcatatgaa tcagttccag tccgaaactc
     1921 gaataagcat cgctttgtgt agccggaaaa ccattgccgt tatcgtatat ctcaagactc
     1981 attctgcccg gttcatctcc attggaaaag tgaattccta tttctccctt cttctttcct
     2041 gtaaaggcgt gtttaaagga attggtcact atttcattta caattaatcc gaacggaatg
     2101 gcctgatcaa tcttcagccg aataggttcc ccgggtggaa caagatcaaa gtgtatctcc
     2161 actcctggtg gagtaatgga ttctccagca ctctcggcga ctgtgctcag atactcaaaa
     2221 agatctaccc atcccaggtt ggactggtac aacttttcat gaacaatggc catggtttta
     2281 atgcgcgact gactctcgag aatgagttta tggagttgcg gatgttcgaa atacgaggat
     2341 tgcagtttta ataaacttga gatgatttgc aggttgtttt ttacccggtg atgaatttct
     2401 ttcagcagta cttccttttc tgccagactt gatttgatgg cctcttcagc ctgtttacgt
     2461 tcaaagaatt gcgggatgga ataaacgtcg ttggagtcat gaatctcact cactcctggt
     2521 agatagtctt taattcggtg aggaggagcc ataacaaagc tgcacttatc gtgacccata
     2581 gcccggcaag taacttcaac agctgtaagc ggtaaaccaa aacttgcctc acaccaaccg
     2641 gaactgtatc cggcattcat aatacaaacc gggaaatcag atgttttacc tgcgtttacc
     2701 caactgtctg cttcgaagga aaaaggatga tcgtacttta aaaggaaatt gtcgtcggga
     2761 ctcggattcg attcgggtga aatatcgaca aatgcccaac cggtatatgc aaaatgaacg
     2821 ggaccggcgg atagtttttc aaccggattt ttaagattca ttaccttatg aaaagccttg
     2881 gcatcctgca atccgatcac atgtgccata tcgaaaagga aattccgtcc gatctcaacg
     2941 gcttcttttt ctcccttatc ggaatagagg tccttgagtt tctcaaaaaa atcgatggat
     3001 aaagctgaag cccgcacaag aatgtaccgc tgaccttcaa tctcgatggt tgcctttgta
     3061 ggttcaattt gaatgccgct gaaatatttt ccaactgttt cctgagcacg ttgaaaaatg
     3121 tcttcgaatg gttcaggcac ttttactgtt tctccattca gatgaatgga ccggatacct
     3181 ttacgcaaca gtgcattttc cctttcgagt aaagcaatgc gcagcaatag atcggaatta
     3241 ttttccggtt tatcctttcc ttttccggcc atttaaccaa caagttaagg aagtttttca
     3301 tcctgcaata cgcaggactt aattttcagg attctcctca ggaacaccct ccacttcggg
     3361 caaaacagga tttgagatca gaacagaatc tttggcaata atacccgaat cggaatagct
     3421 tgcaaaggtc ctgcaattat gaggtttcga aatggtaaca ccgggttcag ggaatttccc
     3481 tttcgacagc aagagctttt tatccgtata aagcagatcc atgaattttc cgtacacagg
     3541 taatgctgtt cgggctcctt caccggtttc ggaagtacgg aaacgaatac ggttatcatc
     3601 tgcaccaacc caaactccgg tcacgtaatc tttggtcaac ccgatatacc aaccatctgc
     3661 ataattgctc gaagtaccgg tcttccctcc tacttcattg ccatgtacaa atttaaattc
     3721 ccatagcgcc tgtgaagtac cgcctggttc ttcaaatccg ccacgcagca tatatgtcat
     3781 taaccaggct gtttcttcgg aaatagctct tctgctttcg ggtttaaatt ctgcaatggt
     3841 attccccttg cgatcggtaa tatgggttac caacatcggt tcattccaaa ttcctttgtt
     3901 catgtaaacc gaataagctc caatcagttc aatcaaagta acatcgttcg atccgagtcc
     3961 gattgatgga accgctttca atggactttt aattcccatt cgctttgcga gttctgctac
     4021 attttcccaa cccacttttt cggtgagcaa ggctgtaacc gtattgatgg atctacccat
     4081 tgcatgccgc aaagtaacca gttgtccgga gttggaccaa tcggcattat ggggtgtcca
     4141 cactttgttt tctccgtttt ccttgtattt gatcgctacg ggaacatccg gtaatttatc
     4201 acagggtccc cagccttttt caattgcggt agtgtaaaca aaaggcttaa atgtggagcc
     4261 cggttggttc atggcaacac taacgtgatc gtatttgaaa taatcgtaat tgactccacc
     4321 aacatatact ttcacatgag cgttggctgg atcgatggta atcattccgc aatgcagaaa
     4381 ggcgatgtag tgtttgatgg aatcgaggga actgtattgc acttcttttt cgccttccca
     4441 ggtaaaaatt ttcattttct ttttttcttc catcgctgcc atcagacttt tctcatcgtc
     4501 aggatatttt tttttgagtt ttttatatac atccgactgt cgcaccagat cccataaaaa
     4561 accatcttta ttctttgcgg tggctttatc ctgccagggt tttcggtcgc tccaatgcat
     4621 tttaaatcgt tgctgcaggg ctttcatctg aatagcaaca gcacgttctg catgcgcctg
     4681 cattcgtgag tcgagtgtgg tatggatgat tagtccgtcc gaaaaaatat tgtagccgga
     4741 ttccttttgc cattcggata atgatttcgc tactgcggtt cggaaataag gggcaattcc
     4801 ttcactgtag gaatattctc tgtattctac acccaaagga gaactgcaat acagatcgtg
     4861 ttcttctgtt gtgatttttc cgtatttgag taattgtccg ataaccgtat ttctccgttt
     4921 caggcaacgc tccgggtttt tgaggggact gtaatacgtc ggggctttca gcaaaccaac
     4981 cagcaacgaa gcctgcggaa cggaaagatc tttgggatac accccaaaat aactatgcgc
     5041 agctacttta attccaaatg tattatgtcc aaaatccacc gtgttgaggt acatcgtgag
     5101 aatatcctcc ttggtgtaga acatttccag tttcactgca gtaatccatt ccttcgactt
     5161 ggctattaca gtatttacat agggcagata acccagtaat ccggttgatc gccgcttcct
     5221 ggttttaaac agattttttg ccaactgctg ggtaagggta cttcctcccc gcttatcacc
     5281 tcttgcggtt gaataaactg caccggcaat actttttaga tcgattcccc aatgcttgta
     5341 aaagcggaca tcttctgtaa caaccaatgc gttgatgatg ttgttgttga ggtcgccaaa
     5401 ctcgatcggt tcacgatttt cctgaaagaa tttccccagc aatactccat cagcagaata
     5461 cacttcagag gctgttggga gatcaggatt ggctaccatg ctaatgctcg gcatggttcc
     5521 gaacagccag agaaaattca gttcaattgc cagaacaaac aaagcaatgc tcaggaagaa
     5581 ggaatacagc agactgagcg gtttaataaa ccatcgtagt gatcggaatt tactccaatt
     5641 gaaaatactg aaccggaaaa tccgcttagc aagcggcgac aacaattcct tgctgagctg
     5701 ggagtgtcga cggataagaa gtaaataatc ggctgctacg ctcatgggct aaagagggct
     5761 gcaaatttga ggaatattct gttcactccc ccacacccaa taagtttttt cccccagttc
     5821 cgcttccatt gccaattaaa ggggaaaagt tccttatttt gtggttccta agagtgcaaa
     5881 attcacccac ataccgcatt atttgtctgt taatgaccgt aattctgttt tacggctgta
     5941 gtcagacccg cagacttgag ccggggcagt atttgctaac caggaacaaa atccagttcc
     6001 agggcgacga cggaaagatc agtcaggccg agattgagta cttactgaaa cagaaaccga
     6061 accgcaaaat cttcaagtat ccttttcacc tttttattca taaccttcca aaccgcgaaa
     6121 aaacagaacg caaatcggaa gcaaaaaaac tggagtgtca gaaacgcaat ctcgaacttc
     6181 aggttaagct cgatacactc aacattgaga ttgaacgtaa tcgaaaagca attgttgggc
     6241 gaaaaggccg cgacagtacg cgcattgcca atgagattta ccgactggaa ataaaaaaaa
     6301 ttgatctgga agacaaaatg gaaaactgca gagccgtttt gggtcaccga atgatgtatt
     6361 ccataggaga agaaccttcc gtactcgatt catcgcgcat ggaaacctct gttcggaaca
     6421 tcaatttgta tttattcaaa aaaggctatt tcaacaacca ggtcagctca ggagtaaaat
     6481 ataccggtaa aaaagcagaa gtaatctata cgattaagtt aggtgctcct tacaagatca
     6541 ggaccatcaa ttaccagatc aaggatcgtc gtttatccag ggaaatacgt agtgcagatc
     6601 ccggcacatt ggttgctgtt ggaaacaggc ttgattacac tattctcgat aaggaaagag
     6661 atcgaattac gactcattta cgggataagg gataccactt tttcagtaag gaatttatct
     6721 atttcgatat tgataccact gttggtaatc tacaggtcga tttggtaatg ggtgtaaaaa
     6781 acctgatccg tacccacccg gaaatcccgg attcgctgat tgaagttccg catcagaaat
     6841 acatcatcgg aaatattttt gtcaataccg atttcgatcc atccattgcc aattcaaccg
     6901 acaaataccg cttagatctc tacgatacgc ttgttggcga taaggaaaca caaggcatcc
     6961 attttattta tctccgtaat aaaggaattt cgatcaaacc caaggtgttg atcgatgcta
     7021 tttacatcaa gcgtctatac gattatcgtg caaaggaagt ggaatacaca tttaaaaaac
     7081 tatcctcact aggagtattc cgtaccatca atattcagtt tgaagtggga gaagaagatc
     7141 ccacaggaaa ttacctgaat tgttatatca atttaagcag attaccacgt caaaacattt
     7201 caatagaatc aaatggtacc aatcgcggag gaaaccttgg tgtagcagga aattttacct
     7261 accgaaaccg aaataccttt cgtggcgcag aaacattccg cttttccatg tcgggcggac
     7321 tcgaagctca acaggctctt gccaatgaag atcagaccat tgttggacga acattcaaca
     7381 ccctcgaatt tggacctgaa attgcgctct atattccaaa attcatcagt cccatccctt
     7441 tttacaaatt cgataaagct gcttctccca gaacagtttt tacggcaagt ttaaattatc
     7501 aggatcgtcc cgactttgtg cgtaccatcc aaaacgtttc ctatgcgtac gaatggaaag
     7561 gcaaaaagca tacctccttt tatcttagtc ctgtaaagct atctgcagta aaaattcgtc
     7621 cggacagtag tttcacagcc cgactcgaag cattgaacga tcagttcctt ctaaactcct
     7681 accgcgatca ctttatcatt ggaaatcagt tcaggtacga atacaacaat caggatccca
     7741 attccagcaa gcgaaactcc ttgtttttta aggtcggatt ggaacaaagc ggaaacttga
     7801 tgcgattggc ctacgaattg gcaggaatgc ctcacgattc agtcggcagt atttttggga
     7861 tacagtttgc ccaatttctt aaggtagatg ccgattttcg tttcacccaa tccttcaata
     7921 aatccagccg ggtggtttac cgtgttgccg gaggagttgg attaccgcaa aagaatttta
     7981 atcaatcttt gccctttgaa aaaagctttt ttgtgggtgg ttccaacgga ttgcggggat
     8041 ggaaagcacg tacccttggt cccggatcgt atatgggtgc acgagtggca tttgataaaa
     8101 taggagatat ttacctcgaa ggaaacgtgg aataccgatt cgatctgttc ggatttttaa
     8161 aaggagcctt atttgccgat gcaggcaaca tctggaacct ccgcgaaaac cccgcacggc
     8221 cgggttctaa atttaccccc aacttttacc aggaattagc attggatggt ggcctgggct
     8281 tacgactcga tctggatttc ttcatcattc gattcgattt tgccataccc cttaaagagc
     8341 cgggattgat ggatggcgag cgttggattt ttcaggataa aacagagtcc aacacatggc
     8401 tcaatgcccg tggatcaggc gattatcgtc cacgccccaa tctcaatctg ggtattgggt
     8461 accccttctg accctgtctg catattgggc aaaaaaaata cttagttttg tcgcccactc
     8521 taaagaatca ggaatggcca ccaacgtaac gcaactcctt ggcaaagacg ccgaatcatt
     8581 actgggtcac cagtgcaaga ctatcgacaa atcgatgctt caactgccgg gtactaactt
     8641 catcgacaac aattttgccg gaaccaaccg caatccacag gtattgcgca gtctggcaag
     8701 catctacaac catggtcgcc ttggaggatc aggatatgtt tccattcttc cggttgacca
     8761 gggaattgag cattctgcag gcgcttcatt tgccccttct ccgatctatt tcgatcccga
     8821 aaacattgta aaacttgcag tagaaggcgg ttgcaatgca gttgcttcca catacggtgt
     8881 tttggcatcc tgctctcgta aatacgcaca caaaattcct tttatcgtta agatcaatca
     8941 caacgaattc ctttcttatc ccaataaata cgatcagatc ctcttcggat cggtggatga
     9001 agcctggaat ctgggtgctg ttgctgttgg agctaccatt tatttcggtt ccgatgagag
     9061 tgcccgccag atcgttgaga tcgcacaggc atttgagcgt gcgcacgaat tgggaatggc
     9121 taccatcctt tggtgttacc tccgtaatcc gggcttcaaa aaagatggtg tggattacca
     9181 taccgcagcc gatttaaccg ctcaggccaa tcaccttggt gttaccattc aggcggatat
     9241 catcaagcaa aaacttcctg aaaacaatgg tggctacaat gccatcaact ttggaaaaac
     9301 acataaaaaa gtatacagcg atctgagcag cgatcaccca atcgatcttt gccgttacca
     9361 ggtagctaat tgttatatgg gacgtatcgg actgatcaat tcaggcggtg cttctaccgg
     9421 ttcaggtgag agtgatttga gtgaagctgt aaaaacagca gtcattaaca aacgcgcagg
     9481 cggacatggt cttatttccg gaagaaaagc tttccagaaa cccatgaacg aaggtgttca
     9541 gttactcaac gctattcagg atgtttacct ggataaaagc gtaagtattg cctaattaat
     9601 aacctgaatt ttgagcgact ctatgggatg gtttaaacga agcaaagaag ggattctaac
     9661 ttccaccaag gataagaaag aaacacccga aggactttgg tacaaatgtc cggaatgcgg
     9721 acacgttgct accaccgaag atcatgccga tcatatgtgg gtttgcgaaa aatgcagcta
     9781 ccacgaaaaa atctctgcag aacagtattt cctgattctg ttcgacgatc atgaattcac
     9841 agagtttgcc gcaaacgtaa tttccggcga tccgcttgag tttgtggata ccaagaatta
     9901 caaagaccgt attaaggata ccattgcaaa atccggactc aaagacgcta tccgctgtgc
     9961 atatggaacc atggaagacc ag
//
LOCUS       DAOMYG010000051         9980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2257_length_9980_cov_8.584383, whole genome shotgun sequence.
ACCESSION   DAOMYG010000051 DAOMYG010000000
VERSION     DAOMYG010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 9980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9980
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2257_length_9980_cov_8.584383"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..653
                     /locus_tag="PK610_02765"
     CDS             126..653
                     /locus_tag="PK610_02765"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRJ34876.1"
                     /translation="MKKIIFAMCLFMASGFTGVIAQKVGHVDFQSIMIALPERKSAED
                     TLKKFAASIQNDMRIMEARYQERVAQYERDKKGGASADVLAVAEQDIMEYQQRIMNFE
                     EKAQERMQMKESELLKPMIDKVKAAVAKVAKAQGVGYVFDISQLIYMEGGIDLTALVR
                     KELGLPAAATTGGGN"
     gene            725..1552
                     /gene="murI"
                     /locus_tag="PK610_02770"
     CDS             725..1552
                     /gene="murI"
                     /locus_tag="PK610_02770"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019976480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRJ34877.1"
                     /translation="MRPKHPIGVFDSGMGGLTVLKEIVKRLPDYDYLYLGDNARAPYG
                     PRSFDTVYEFTLQSVKQLFAMGCPLVILACNTASAKALRNIQQKDLPKIAPDNRVLGV
                     IRPTAEIVGSLTKNKKIGVLGTQGTVNSNSYPIEIAKFSQDVSVTQEACPMWVPLVEN
                     NEHLSVGADYFVKKHLDHILHSDPGIDTIILGCTHYPLLLDTIKKFLPSNIQLIAQGE
                     IVAEKLEDYLLRHPEMEQRISKEGKREFYTTDSPEQFDASAKIFFGEKIKSRHLHLD"
     gene            complement(1553..2068)
                     /locus_tag="PK610_02775"
     CDS             complement(1553..2068)
                     /locus_tag="PK610_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008638739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRJ34878.1"
                     /translation="MALIKSVQGKSPKFGKDCYIAENATIVGDLECGDQCSFWFNAVV
                     RADVHFIRMGNKVNVQDGACIHCTYQKAPTTIGNNVSIGHHAIVHGCTIHDNVLVGMG
                     AIVMDNCVIESNSIIAAGSVVLEGTRVESGSVYAGIPAKKVKEVNKELFEGEIQRISN
                     NYVMYSGWFKE"
     gene            complement(2072..2425)
                     /locus_tag="PK610_02780"
     CDS             complement(2072..2425)
                     /locus_tag="PK610_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HRJ34879.1"
                     /translation="MKTIIKNSPIILLFILLSQTGFGQKKMAEATIKTSIVCDMCKTT
                     IEKNLIFEKGVKEVRVDIEKKEVYVRFRTDKTSIDLIKQALVKLGYRADDLPADPKAH
                     AKLPDCCKKEGCGKD"
     gene            complement(2454..4670)
                     /locus_tag="PK610_02785"
     CDS             complement(2454..4670)
                     /locus_tag="PK610_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ34880.1"
                     /translation="MKKHIIYLILYGSFLIPEVFGQEVKGIVYCESTQGKKETLPGAH
                     IVWLNAGSGVITGKDGTFSIPFKGPDKLIVSFTGFASDTVLIDSAFVFVETILRDPMM
                     LQDVEVTARSYSTSISMIKPFNMEVIGQAEIKKAACCNLSESFESNASVDVVYGDAVS
                     GAKAIQMLGLSGVYTQMQIENVPLIRGMSASFGMQYIPGTWLESIQITKGVGSVVNGY
                     ESMSGQINVELLKPENDKVERWFFNLYTNHLGREEANIHYRKKINDKLSTLILAHGSG
                     YFFENDQNRDGFRDNPLGRQLNVMNRWKLQGKKTEQVFGIHAVYDNKNGGQLGFVPSQ
                     HQFTYDKFGVGISTQMIEGFFKNGFLFPSSPTKSMGLIISGKHHTQDTYYGLRSYFAE
                     QRSAYTNLIFQDIIGTTMHNYKLGLSTMLDDYRERYRDSLYNTLEIVPGVFGEYTYSY
                     LEKFQLLIGARYDFHNRFGGVFSPRAHIRFSPNEHLTIRLTGGQGFRSPRIFMENVAP
                     LASARQVVITEELGMERSTNFGGSITTRFKFLKRESSFNIDYYHTRFENQVIADMDLD
                     AHKIVFYNLKGESFSNSLQAEVDLSLYKGLSVKIAYKRYQVMVTYHDTLMERPMVPRD
                     RVMMNLSYETPSKKWKFDLISNYYSSSRLPNTESMTSDMRFPSRSQEYYIVHFQITKQ
                     FRKFEWYIGGENLLNFVQQQAIMSADNPFGPHFDASMIWGPLNGRTFYSGIRISIR"
     gene            5285..5908
                     /locus_tag="PK610_02790"
     CDS             5285..5908
                     /locus_tag="PK610_02790"
                     /inference="COORDINATES: protein motif:HMM:NF020295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRJ34881.1"
                     /translation="MENDIRITSVYAEMTPNPSTMKFVADRPIVQIDGIAEYASAEEA
                     KGSSLLAEQLFKFPFVKGVFITTNFVTITKTDAISWDFITMELREFVMDFLRKNLFAV
                     DKFPEPKTGEGEVKKFKMLGKASHAQPTSEIDSKIISVLDEFVRPAVENDGGAIHFQS
                     FKEGIVSLVLRGSCSGCPSSMGTLKHGVEQLLKQMVPEVVEVVAVED"
     gene            5979..6686
                     /locus_tag="PK610_02795"
     CDS             5979..6686
                     /locus_tag="PK610_02795"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ34882.1"
                     /translation="MKNLTSLLFLILVLIGHSTKAQNDGSIRGQVLDSLTKEPAVGAN
                     VYVMIGDQMRGTTTDIDGRFHLKPLVPGTYNLVVSYMEQKKTIQVKVNPNQITMMSDV
                     LLTDNLLGEIEVTVYRDPLINPEETSVTTIRYEQIKNNPHIRDINKLAGTIGGVQVSE
                     TGQANVRGGRSDAMIYFVDGVKVADGNIRLPGVAIGSLTVYTGGVPAKYGDVTSGVII
                     VETRSYFDLWAEHNKGK"
     gene            6761..7339
                     /locus_tag="PK610_02800"
     CDS             6761..7339
                     /locus_tag="PK610_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ34883.1"
                     /translation="MIFRKKHTNTQSDEELVRKYASSADVQIVGLLYERYSHLVFGVC
                     LKYLRNRDDSKDAVINIFEKLLSDLKNHQVDNFKSWLHSVARNHCLMILRKSNRPGTE
                     KEIETLEHILAEDQNDTDVFAKESSLQELESAICKLNKEQQTCIELFYLKEKSYQEIT
                     TQTGYSLNAVKSHIQNGKRNLKNMLSNHEKAI"
     gene            7323..8441
                     /locus_tag="PK610_02805"
     CDS             7323..8441
                     /locus_tag="PK610_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ34884.1"
                     /translation="MKKQSNTIFSSRETPNREETQRWLDGKGDAALNRKMEEQLAEDD
                     LTAEGLDGLSADPEALSVLPDNLFKTNTHASKGTSIYKILFWPTLAIACISIVVLVAN
                     QSEKSNEKTAHHTPLTESGTPITSSTEIDQKEIDDAEPISEPEQITYAKTKADQPKTV
                     ILQKTEVEINPVKENEALLTPVETKKADNIESGTDEKYARSNVRFTYFNDLKVIDYGD
                     LYTTDIKKTEIVLTGTPANIGDTSTTNTEVETRTVYIPYEKFLRESMGEFSRNQYKTA
                     LKNYITIIQHYPDDLNAHFYGGLCYYNLGKFDKGIQSFDKCIHNAFSTFEEEAEWYKA
                     LSLIKLSKKENAKELLQIIIAKKGFYSERASKQLAELN"
     gene            complement(8438..9937)
                     /locus_tag="PK610_02810"
     CDS             complement(8438..9937)
                     /locus_tag="PK610_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34885.1"
                     /translation="MDNFKEIISTFSDKDMREFRYFLVRHRPKSERKDLELLQELAEG
                     EIIKDRKGKDDYHSLRKRLMRQLSEFVVLKQLQDDVTETSSVLGWVSMARYLFEKRMN
                     ATAWKYLLRAEKSAIASERYDLLNSVYLLMLENAHTSSHYPLEFLIKQMEDCKIRADE
                     DERLLKATAMIRQRMEEAKLSGNIFGLSDFVEATLDRFQLGSAIMNRPIHLFRIMEII
                     RSTYLAVRDLSNFESLVLEQYTVFSKAAGDSPHYQYFRLNFIYMIAHVLYHNRKYKES
                     DQWLEKLEAEMKKNSGRHERQFAARLVSMRSSIYSFTGRNDEAIAILEGVLEDGIRKF
                     DFEDILNLKLNLVFCFFLKGEFRKANRVFVQFGQHSDSFLQKKMGQEWLLRSKMIQLI
                     VQYELGNEDVSLTMIRSLERDHSDFLDQPQYIKAKNYLQFLKNYMKDPFATSFSDFHQ
                     KALQELFVLPAGTEESKAIAFYCWLKAKLIGRDYYSVLLEEVAAKLDEN"
BASE COUNT         3147 a         2111 c         1890 g         2832 t
ORIGIN      
        1 gaggatatac ttttattttt gacaaagcca attcggcatt cattttgtat gcagacccaa
       61 aggcggacaa gaccgacgcc gtaatgaaga aactcggact ttaatacaca tcaacaaaaa
      121 gtaatatgaa gaagatcatt ttcgcaatgt gtttatttat ggcttcaggt tttaccggag
      181 tcattgctca aaaggttggt cacgtcgatt ttcagtcgat tatgattgct ctacctgaaa
      241 gaaaatctgc agaggataca cttaaaaaat tcgccgcatc catccaaaac gatatgcgca
      301 ttatggaagc gcgttatcag gaaagagttg ctcaatatga gcgtgacaaa aaagggggcg
      361 catctgctga tgttcttgca gtagctgaac aggacataat ggaataccag cagcgcatta
      421 tgaactttga agaaaaggct caggaaagga tgcagatgaa agaatcagaa ctgcttaagc
      481 ctatgatcga caaagtaaaa gcagccgttg caaaagttgc aaaagctcag ggtgtaggtt
      541 atgtatttga tatcagtcag ctgatctata tggaaggtgg aattgacctt actgctttag
      601 tgcgtaaaga actcggatta cctgcagcag ccacaaccgg cggcggaaac taatctcact
      661 taatttcctt caacccccga atgaacttcg ggggtttgtt ttttctgatt gattatattt
      721 gaatgtgcgt ccaaaacatc ctataggtgt attcgatagc ggaatgggtg gattaaccgt
      781 tctgaaagaa attgtgaaac ggttacccga ttacgattat ctctacctgg gagataatgc
      841 aagagctcca tacgggccac gttcgttcga tacagtttat gaattcacac tccaaagcgt
      901 taaacaactt ttcgcaatgg ggtgtccgct tgtaattctg gcctgtaata cggcttccgc
      961 aaaagcccta agaaacatcc agcaaaaaga tcttccgaaa attgctccag acaaccgggt
     1021 attgggcgtg attcgaccta cagctgaaat tgttggttca cttacaaaaa ataaaaaaat
     1081 tggtgtttta gggacgcagg gaaccgtgaa ctccaactct tatccgattg aaattgcaaa
     1141 attcagtcag gatgtgagcg tcacacagga agcctgtcct atgtgggttc ctttggtgga
     1201 aaacaatgaa catttaagtg tgggtgcaga ttatttcgtg aaaaaacacc tcgatcatat
     1261 tctccattca gatcccggaa ttgacaccat catattaggc tgcacacatt acccgttact
     1321 actagatacc atcaaaaaat ttctgccaag caacattcaa ctcattgctc agggagaaat
     1381 tgttgctgaa aaactggaag attacttatt gcgtcatccg gaaatggaac aacgcataag
     1441 caaagaagga aaacgagaat tctacacaac tgactcacct gagcaattcg atgcttcggc
     1501 aaaaatattc tttggagaga aaataaaatc caggcatttg cacttggatt gattattcct
     1561 taaaccaacc ggaatacatc acgtaattat tgctgattcg ctgtatctct ccttcaaata
     1621 attctttatt cacctcttta accttttttg caggaatacc ggcgtacacg cttccgcttt
     1681 ccacccgtgt accctcgagc actacgctac cggcagcaat gattgaattc gactcaatca
     1741 cgcaattatc cataacaata gctcccatgc caaccaaaac attatcgtga atggtacaac
     1801 catgaacgat ggcatgatga ccaatcgaaa cgttgtttcc aatggttgtc ggagcctttt
     1861 ggtaggtaca atggatacat gccccatcct gaacattaac cttgttgccc atgcgtatga
     1921 aatgcacatc cgcacgaaca acagcgttaa accagaaact gcattgatca ccacattcga
     1981 gatccccaac aatagttgcg ttctcagcga tgtaacaatc cttgccaaat tttggggatt
     2041 ttccttgtac agatttgatc agtgccatat tttaatcttt tccgcaacct tcctttttac
     2101 agcaatccgg caacttcgca tgagctttcg gatcagcagg taaatcatcg gcgcggtatc
     2161 cgagttttac aagggcttgt tttatgagat caatcgatgt cttgtcggta cggaaacgaa
     2221 cataaacttc cttcttttct atatcaacac gaacctcttt gacacccttt tcaaaaataa
     2281 gatttttctc aatggtagtt ttacacatgt cgcatacaat agatgtcttt atggttgctt
     2341 cagccatttt tttctgccca aagcctgttt gtgacaacaa tataaacagt aaaatgatcg
     2401 gcgaattttt tataattgtt ttcataagga tttttttagg ttcattttat tttttatcta
     2461 attgagattc tgattccgga atagaacgtt ctgccattta atggtcccca aatcatactc
     2521 gcatcaaaat gcgggccgaa aggattatct gccgacatta ttgcctgctg ctgaacaaaa
     2581 ttcaagaggt tttctccacc aatataccac tcgaatttgc gaaactgttt ggtgatctga
     2641 aaatgcacaa tgtaatactc ctgcgaacgg gaagggaatc gcatatcaga agtcatagat
     2701 tcagtattgg gcaaacggct actgctataa taattactta taagatcaaa cttccacttc
     2761 ttggatggcg tttcataact cagattcatc ataaccctat ctctaggcac cattggacgc
     2821 tccattagag tgtcgtgata ggtgaccatc acctgatacc ttttgtatgc aattttcaca
     2881 cttagccctt tgtacaagga taaatctact tcggcctgca aactgttcga aaagctttca
     2941 cctttcaaat tatagaacac aattttatgg gcgtccaaat ccatatcagc aatgacctgg
     3001 ttttcgaatc gtgtgtggta ataatcaata ttaaaactgc tctcacgctt taaaaattta
     3061 aaccgcgtgg tgatagaacc cccaaaattg gtgcttcgct ccattccaag ttcctctgtt
     3121 attacaacct gtcgagcact tgccaagggt gcaacattct ccataaaaat gcgtggtgaa
     3181 cgaaatccct gtccacccgt caaccgaata gttagatgtt cattggggct aaatctgata
     3241 tgcgcacgtg gtgaaaaaac gccaccgaac cgattgtgaa aatcatacct ggctccgatc
     3301 agtaattgga atttttcaag ataggaatac gtgtattcgc caaagactcc cggaacaatt
     3361 tcaagagtgt tatacagcga atcgcgataa cgttccctgt agtcatccag cattgtactc
     3421 aatcccaatt tataattgtg cattgttgta ccgataatat cctgaaaaat gagattggtg
     3481 tatgcagaac gttgttcagc aaaatagctc cgtaaaccat aataggtatc ctgagtatga
     3541 tgcttgccgc taatgatcaa acccatgctt tttgttggag aagaaggaaa taaaaagccg
     3601 ttcttaaaaa agccttctat catctgtgta cttataccta ctccaaattt atcataggta
     3661 aattggtgtt gtgaagggac gaagcccagc tgtccaccat ttttattatc gtaaacagca
     3721 tgaattccaa atacctgttc cgttttcttt ccttgtagct tccaacggtt cattacattt
     3781 aattgccgac caagaggatt atcacgaaag ccatcccgat tctgatcatt ttcaaaaaag
     3841 taaccggaac catgagcaag gatcaatgtg ctcaatttgt cgtttatctt tttgcgatag
     3901 tgaatgtttg cttcttcccg tcccaggtga ttggtataca aattaaaaaa ccatcgctcc
     3961 accttgtcgt tttcaggttt cagcagttcc acattgattt gtccgctcat tgattcgtaa
     4021 ccgtttacaa cactacccac tcctttggtg atttgaatcg attcgagcca tgttccggga
     4081 atatattgca taccgaaact tgcagacata cctcgaatga gtggcacatt ctcaatttgc
     4141 atttgagtat aaactccgct caatcctaac atttgaatcg cctttgcacc cgaaacagca
     4201 tcaccatata caacatcaac gcttgcgtta gattcaaaac tttcggaaag attgcaacaa
     4261 gctgcttttt taatttctgc ctgaccaata acctccatat taaagggctt gatcattgaa
     4321 atacttgtgc tgtatgaacg cgctgtaact tctacatcct gcaacatcat tggatcgcga
     4381 agaattgttt caacaaatac aaacgcactg tcaattaaaa ctgtatcaga tgcaaacccc
     4441 gtaaacgaaa ctattaactt atcggggcct ttaaagggaa tggaaaatgt cccgtctttt
     4501 ccggtaatta ctccggatcc tgcattgagc caaacaatgt gtgcaccagg cagcgtttct
     4561 ttttttccct gtgtgctttc acagtaaacg atgcctttaa cttcctgccc gaaaacctcc
     4621 ggaatcagaa atgatccata tagaattaaa tatataatat gctttttcat gttcatgttg
     4681 attaaaataa ccgaataacc cttatccgct tggatgaggc aacatgaacg cttaaattct
     4741 gaaaacctga tttcgaacca aaatatctcg tcctgacaac tcaggcggtc cgtttggaag
     4801 gtggttttcg tatactgtag aaattgttga ggatacgaat ttacaacccg aaactggggg
     4861 gataaaaaaa ctatcctgtt gaaacgactt taaagtagaa aatgcgtgga aattcaattg
     4921 ctggctaaac gatgattcag agcaacaact cttttttttc ctgattactt ttttgcttgg
     4981 ggattttgat gaacaacaag tcttgccttc tgtttcgatc ttttcggcac agcaatcatc
     5041 aaccggagcg aacaaactat atgaaacatc accggaaatc aagcacttat gtctgaaaat
     5101 ggtaagccca ctcagtgaga acaaaaccgt taatagtaaa acggcagaaa tactatatcg
     5161 caaaatgctg cgcatcatct atttagtaac gtaaaaataa gggaaagttg ccccaacaca
     5221 gaaacaattt acctgttttg ttgtattcct gactacaatc tcttgaaatc cataattttg
     5281 ttgcatggaa aacgatatcc gaattacctc ggtttatgcc gaaatgacgc ccaacccttc
     5341 aactatgaaa ttcgtagctg accgtccgat tgtacaaatt gacgggattg cagaatatgc
     5401 ttctgctgag gaagcaaaag gatcttcttt acttgcagaa caattgttta agtttccctt
     5461 tgtaaagggt gtttttataa cgaccaattt tgtgacaatc acaaaaacag atgcaatctc
     5521 atgggatttc atcactatgg agctaaggga atttgtgatg gattttttgc gtaaaaatct
     5581 ttttgcggta gataaatttc cggagccaaa aacaggcgaa ggagaagtaa agaagttcaa
     5641 aatgcttggt aaagcttcac acgcacaacc aacctccgaa attgattcaa aaatcatctc
     5701 cgttctggat gaatttgttc gtcccgcagt tgaaaatgac ggtggggcta tccatttcca
     5761 gtcctttaag gaaggtattg tcagccttgt gttaagaggg tcctgcagcg gatgtcccag
     5821 ctcaatgggc accctcaagc atggcgtgga acagcttctg aaacagatgg ttccggaggt
     5881 agttgaggta gttgcagtgg aagactaaaa atatttcttt ggggcgttat ggaaattgtt
     5941 tcaaccctgc atcaaagggg aaacaaaatt taaacgccat gaaaaatctt acctctctgc
     6001 tattcctgat actcgtattg atcgggcata gcaccaaagc gcaaaacgac ggaagtattc
     6061 gcggacaagt cctcgacagc ctcacaaaag aaccggcagt tggagccaat gtatatgtca
     6121 tgatcggtga ccaaatgcgt ggaacaacaa ccgatattga tggcagattt catttaaagc
     6181 ctcttgtccc tggaacttat aatctggttg tttcctatat ggaacaaaag aaaaccattc
     6241 aggtgaaagt taatcccaat cagataacca tgatgtctga tgtactgcta acggataacc
     6301 ttttaggaga aatagaggta acggtatatc gagatccatt aatcaatccg gaagaaactt
     6361 ctgttacaac catccggtat gaacaaataa aaaacaatcc tcatatccgc gacatcaaca
     6421 aactcgcggg caccatcgga ggtgttcagg taagtgaaac cggacaagcc aatgttcgcg
     6481 gaggaagatc ggacgcgatg atctattttg ttgacggagt aaaagtggcc gacggaaata
     6541 ttcgcttacc cggtgttgcg attgggagtc ttactgtata cacaggaggt gttccggcaa
     6601 aatacggtga tgttaccagc ggtgtaatca tcgttgaaac cagaagctat ttcgatcttt
     6661 gggcagaaca taataaaggc aagtgatcac caatgcaagg acgggaagga aggatcccgt
     6721 tcttgctttt tttgtcgatg tgaatcgtat attgcagtga atgatattca gaaaaaaaca
     6781 taccaataca caatccgacg aagagctggt gcggaaatat gcttcttcag cggatgttca
     6841 aatcgtgggt ttgctttatg agcgttattc gcatttggtt tttggtgtat gtttaaaata
     6901 cttacgcaat agggacgaca gcaaagacgc tgtcattaac atcttcgaaa aattactctc
     6961 cgatcttaaa aaccatcagg ttgacaactt caaatcctgg cttcactctg ttgcacgaaa
     7021 tcattgttta atgattcttc gcaaatccaa cagacccgga acagaaaaag aaattgaaac
     7081 acttgaacat attttagcgg aagatcaaaa cgatactgat gtgtttgcga aagaaagcag
     7141 cttacaggaa ttggaaagcg ctatttgcaa actcaataag gagcaacaaa cctgtatcga
     7201 actcttctac ctgaaagaaa aaagttatca ggaaataact acccaaacag gttattctct
     7261 gaacgccgtt aagagtcata tccaaaacgg caaacgcaac cttaagaata tgctaagcaa
     7321 tcatgaaaaa gcaatctaat acaatttttt cctcaaggga aaccccgaat cgggaagaaa
     7381 ctcagcgctg gctcgatggg aaaggtgatg ctgcattaaa tcgtaaaatg gaagaacaat
     7441 tagccgaaga cgatttaaca gctgaaggac ttgatgggct atccgctgat ccggaagcat
     7501 taagtgttct tccggacaat ctattcaaaa caaatacaca tgcatcaaag ggcacatcta
     7561 tttacaaaat tctcttttgg ccaactcttg caattgcttg tattagcatt gtagtattgg
     7621 ttgccaacca atcggaaaag tcaaacgaaa aaactgcgca tcatactcca ttaacggaat
     7681 ccggtactcc aataacaagc tcaactgaaa tagaccaaaa ggaaattgac gatgcagagc
     7741 ccatttcaga gcccgaacaa atcacatatg ccaaaacaaa agcagatcag cccaagactg
     7801 taattctcca aaaaacggaa gttgaaataa atccagtgaa agagaacgaa gccttactta
     7861 caccggtcga aacaaaaaaa gcggacaata ttgaatccgg taccgatgaa aaatatgcca
     7921 ggtcgaatgt tcgctttacc tatttcaacg atctaaaagt tattgattat ggtgatttgt
     7981 atactaccga cataaaaaaa actgaaatag tcctaaccgg aactcctgct aatattgggg
     8041 atacttcaac aactaataca gaagttgaaa cgagaacagt ttatatccct tacgaaaaat
     8101 ttcttcgtga atcgatgggg gaattttcaa gaaatcagta taagactgca ttgaaaaatt
     8161 atatcaccat tattcagcat tacccggatg acttaaatgc gcatttttac ggaggtttgt
     8221 gctattataa tctgggtaag ttcgacaagg gaattcaatc gtttgacaaa tgcatccaca
     8281 atgctttctc cactttcgaa gaagaagctg aatggtacaa agcacttagc cttattaagc
     8341 tttcgaaaaa agaaaatgct aaagagctgt tgcaaatcat tattgcaaaa aaaggattct
     8401 attctgagcg ggcatccaaa caacttgccg aactgaatta attttcgtca agcttagcag
     8461 ctacctcttc tagtaaaaca gagtaataat cccttccgat tagtttagcc tttagccagc
     8521 aataaaatgc aattgcttta gactcctcgg ttcccgcagg caaaacgaat aattcctgca
     8581 acgccttctg gtgaaaatcg gaaaaacttg ttgcgaatgg atccttcatg taatttttta
     8641 aaaactgcaa ataatttttt gcctttatat actgaggctg atccagaaaa tccgaatgat
     8701 cccgttcgag cgaacgaatc attgtaagag atacgtcttc attacccaat tcatattgaa
     8761 cgattaattg tatcattttg ctacgcaata accattcctg acccattttt ttctgaagaa
     8821 aagaatcaga atgttgtcca aactgtacaa aaacacgatt ggcttttctg aattcgccct
     8881 tgagaaaaaa acagaataca aggtttagct ttaaattgag aatgtcttca aaatcaaatt
     8941 tccttattcc atcttcaaga actccttcga gaattgcgat ggcctcatca tttcttccgg
     9001 taaaagaata aatacttgaa cgcattgaaa ccaagcgtgc tgcaaactgc ctctcatgtc
     9061 tccctgagtt tttcttcatt tcagcttcta atttttccaa ccactgatcc gactctttgt
     9121 actttctgtt atggtaaagt acatgggcaa tcatataaat aaagtttaat ctgaaatatt
     9181 gatagtgagg ggaatctcca gctgccttgg aaaaaacagt atactgttcc aaaacaagac
     9241 tttcaaaatt tgataaatcg cggaccgcaa ggtaggtgct cctgataatt tccatgatcc
     9301 ggaaaagatg aattggtcta ttcataattg ccgaacctaa ttgaaaacga tcaagtgttg
     9361 cttcaacaaa atcggacaaa ccgaatatgt taccgcttaa ctttgcttct tccatccgtt
     9421 gcctgatcat tgctgtcgcc ttaagcagcc gctcatcctc atcagcacga atcttgcagt
     9481 cttccatttg cttaatcaaa aattcaagtg gataatgcga tgatgtatga gcattctcca
     9541 gcattaaaag gtaaacacta ttcaataagt cataccgctc tgatgcaatt gcgctttttt
     9601 ctgctcgtaa taaatacttc catgcggtag cgttcattcg cttttcaaat aaataacgcg
     9661 ccatggaaac ccaacctaaa acggaagaag tttctgtaac atcatcctgc aattgcttca
     9721 acacaacaaa ctccgataat tgtcgcatta gtcgtttccg aagggaatga taatcatcct
     9781 tcccctttct gtccttaata atttcccctt ctgctaactc ttggagcaat tcaagatcct
     9841 tgcgctcaga ctttggtcga tgccgaacca aaaaatacct gaactcacgc atgtccttat
     9901 ctgaaaatgt gctgattatc tccttaaaat tgtccatttt attcgaccgt tatataaata
     9961 ctaatatact ctaattcaat
//
LOCUS       DAOMYG010000052         9906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2275_length_9906_cov_9.060806, whole genome shotgun sequence.
ACCESSION   DAOMYG010000052 DAOMYG010000000
VERSION     DAOMYG010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9906
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2275_length_9906_cov_9.060806"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PK610_02815"
     CDS             complement(<1..320)
                     /locus_tag="PK610_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34886.1"
                     /translation="MSINVNLLEELDNLSKIHNLEREDIDAMMAEFAKRILHALRIDR
                     MNVWLFGDDQQQLVSVGEYDLRSHYFKKDSVLLRKNFPAYFKALEENKIILAKNIHTH
                     PATK"
     gene            509..970
                     /locus_tag="PK610_02820"
     CDS             509..970
                     /locus_tag="PK610_02820"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ34887.1"
                     /translation="MSSISPRYTLDLMESPVSIKSPAGVTDREAHDRFERSLDLFHIG
                     VNFEEEGQPEKAIAFFSESIDANPNMSLALLRRGKLFLESGMYNEALADFFSYRNQQP
                     QCPRVLLYLSRLSAVLYEETKDEFHKNNAARYLDWAVQKNLHYSMFRIDEN"
     gene            complement(951..1367)
                     /locus_tag="PK610_02825"
     CDS             complement(951..1367)
                     /locus_tag="PK610_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34888.1"
                     /translation="MKTLDTPLAILCYNDDVMMVDAKPVDMELEGFRDLLSTAIELAE
                     RKPYYCIVDLRKLNYAAPETRSCYAEKFFLEYRIADALVVNSLPLRLLANFYIRVNKP
                     AIPTRMFNDVDKAKDWIEGLKKIKQMNYESVNSHQS"
     gene            complement(1377..2732)
                     /locus_tag="PK610_02830"
     CDS             complement(1377..2732)
                     /locus_tag="PK610_02830"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRJ34889.1"
                     /translation="MLQRLMSNGIHAELSPNEGRKVRLLNIMALVGLACFLFLLVINL
                     AKGSPVVFLVTNLSGQLILVLTLVFNSLRYYRIARWFCLTFFLVFFNGVSFLLGPESG
                     IEYLNILLCVCPLVFFDSLREIIVMSTLSLIGFLISKLGYASFEPVFQNPNIKVIYVI
                     NMVILFVSSFLLLFFYRNELLRYSLIVENQNLQLGAKNKEITDSMNYARRIQNALMRF
                     DKGIESALPESFILFRPKESVGGDFFWFHREDDKIWAACVDCTGHGIPGAFMTMIGIT
                     LLNDIVVERSIREPALVLDELNSGITRLFKQREEEQRVMRDGMELTFLELDLNTRVLR
                     YACANTMFAFVRGTSFTILKGDNQPIGWHIGPVKPFTTNRIQMEKGDQLFLFSDGFSD
                     QFGGELGKKFLFSRLRKLLMEVSKQPAIVQKEQIIERFEAWKAELDQVDDVTLIGAKI
                     P"
     gene            complement(2792..4099)
                     /gene="rimO"
                     /locus_tag="PK610_02835"
     CDS             complement(2792..4099)
                     /gene="rimO"
                     /locus_tag="PK610_02835"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRJ34890.1"
                     /translation="MKTRTLKKNKVNVVTLGCSKNLFDSEVLMGQLKANAFDVEHESN
                     ADDASIVIINTCGFIDNAKQESIDTILQFTEAKKKGLVEKVYVTGCLSERYKPELEKE
                     IPEVDAYFGTRDLPRLLKTLKADYKKELVGERLLTTPSHYAYFKIAEGCDRPCSFCAI
                     PLMRGGHVSTPMEELVDRAKKLAKNGTRELLLIAQDSTYYGLDLYKERKLAELMRRLS
                     DVEGIDWIRLHYAFPSGFPEDVLDVIRERESVCNYLDMPLQHISDPVLKSMKRGTTKQ
                     KTIDLVNKIRDKVPGIAIRTTLISGYPGETLDDHQQMLEWVGQSRFDRLGVFTYSHEE
                     NTGAYKLNDDVPQEEKQRRANEIMELQLGISAGINEEKVGQTLKVLIDRKEGQHYIGR
                     TEFDSPEVDNEVIIDADFLRVGDFVQVKVESAGPYDLHAKPIN"
     gene            4178..6193
                     /gene="uvrB"
                     /locus_tag="PK610_02840"
     CDS             4178..6193
                     /gene="uvrB"
                     /locus_tag="PK610_02840"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRJ34891.1"
                     /translation="MEFKIISDFYPTGDQPEAIRQLSEGLRRGEPAQTLLGVTGSGKT
                     FTIANVVQEVKRPTLVLSHNKTLAAQLYAEFKQFFPDNAVEYFVSYYDYYQPEAYIPV
                     TDTYIEKDLAINDEIEKLRLAATSALLSGRNDVIVISSVSCIYGMGNPAEFSKGKVTI
                     KKGQVIARNQFLHKLVDGLYSRTEADFNRGNFRVKGDTIDIWLAYADHALRVVFWGDE
                     IESIETIDPMNYRTIEKFEVFNIFPANIFVTSRDVIKTATWEIQQDMVKQMEYLKEIG
                     KHIEAKRLEDRVTFDLEMIRELGYCSGIENYSRYFDRRNPGSRPFCLLDYFPKDFLMV
                     IDESHVTVPQIRAMYGGDRSRKVNLVEYGFRLPAALDNRPLKFEEFETLVQQIIYVSA
                     TPAEYELEKSGGVVVEQLIRPTGLLDPVINVRPSGNQVDDLLEEIAVVTERDERVLVT
                     TLTKRMAEELAKYLERMGIRCRYIHSDVDTLERVEILHDLRKGMFDVLIGVNLLREGL
                     DLPEVSLVAILDADKEGFLRSERSLVQTVGRAARNVNGRVIMYADKITDSMRKTIEET
                     NRRREKQILYNTEHGITPTQIVKSLESPLAFGRTRPEDKVMPYSGEEIIHSAADPVMQ
                     YLTKDELLLHIEKVKKTMEGEAKQENFIEAARLRDELFGLMELLKTK"
     gene            6195..6644
                     /locus_tag="PK610_02845"
     CDS             6195..6644
                     /locus_tag="PK610_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34892.1"
                     /translation="MRVFLFFILIGILPACKLKKQSKDKTEATTTMQNHPEVETITDY
                     QRPSNPASLTIVGAKIEGDLLHIDVSYSGGCTEHSFRLIFSGNYKKSMPPQASVYLEH
                     DAKDDTCRSLVEKSLIFNIRKMRFPPEKPGTLIISIEGWNEQLKYVY"
     gene            6699..7964
                     /locus_tag="PK610_02850"
     CDS             6699..7964
                     /locus_tag="PK610_02850"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HRJ34893.1"
                     /translation="MITNRTVRQLTLIVVGILATFSIGGGIIALLAHNWDDFPKVVRI
                     ILSFIPAILGIATFLYSESKYRDSLTWAECSSVFLALMIGSTIGLVAQVYNMGGSFED
                     FLFVWLVMILPIIYLGNSSATAIFYIGLSLTWLYLTYLNSFRFFFGFGSGAGNDVLWF
                     WILFLGVVPHLRYHLQPGRISFRGVTLGWSVAIALCMAAFIGFSGHRLINSTLLITLV
                     YYIGKEYFGNGNSAWQRPFQSISILSIFLMTLIYSGHGLLRFTLERDGYIQSYYGAFD
                     REWQGEGIPATPYFYYFNLVLLFAMAFGAGYFFWREKERNSDYNKAILVFPGMILLAI
                     LLQLFEFETTTRILINLYLLFMAWWYLREGWRMRLGWLVFFAALVFSTTMIGRYFDMD
                     IPFWIKGMMYLGLGAGLLWFNKIYSEEKA"
     gene            7961..8512
                     /locus_tag="PK610_02855"
     CDS             7961..8512
                     /locus_tag="PK610_02855"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HRJ34894.1"
                     /translation="MNRKFILLIVLGLLLAVPIGMVIRNEMLLADEGSIYKFTLQPID
                     PSDPVRGKYIILRYNTMIELDEPIQLQNGEPIYVEVKKDENGWAYFSNVSTTPPDHSA
                     YFKTTVNYGYERTVNINVPFDKYFLNENLAPLAESSYAELTRHGEEFYAEVSITNGEA
                     LLRGVYYMNRPLIDHLREMQVDQ"
     gene            complement(8506..9228)
                     /gene="bshB1"
                     /locus_tag="PK610_02860"
     CDS             complement(8506..9228)
                     /gene="bshB1"
                     /locus_tag="PK610_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HRJ34895.1"
                     /translation="MKIDLLAFAAHPDDVELSASGTLLLHKAQGKTTGIIDLTRGELG
                     SRGNMDLRSEEAQNASAILGIDVRENLGMADGFFEINEASLLKVIEVIRKYRPEIVLA
                     PALSDRHPDHSRAAELVSRACFLSGLVKIESQFGGGAQSAWRPKAVYHYIQDRIEDYD
                     LVIDITPYWEKKMESILAYSSQFHTGKDSSGEPQTPISSKEFMEYLKAKAIAFGRPIQ
                     VMYAEAFKVERTPGVHSLFDLY"
     gene            complement(9309..9895)
                     /locus_tag="PK610_02865"
                     /pseudo
     CDS             complement(9309..9895)
                     /locus_tag="PK610_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790116.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
BASE COUNT         2819 a         2158 c         2033 g         2896 t
ORIGIN      
        1 tcttttgttg caggatgggt gtgaatattt ttggcaagaa tgattttgtt ttcttcaagt
       61 gctttaaaat atgcgggaaa attctttcga agcaaaaccg aatccttttt aaaatagtgg
      121 ctgcgtaaat catactctcc taccgatacc aactgttgct gatcatctcc aaacaaccac
      181 acattcattc ggtcaattcg taatgcatgc aaaatccgtt ttgcaaattc agccatcatg
      241 gcatcaatat cctcccgctc cagattatgg attttggaaa ggttatcgag ttcttcaagc
      301 aagtttacat tgatactcat agctggaatt tgaaatgaat ataaccatca tatcactgaa
      361 aaacaaataa ttatagcaaa ttgttgtaaa aagtcctccg ttttcccgat tcaaatccct
      421 gcaatcgtca accttattaa aaacaaactg ttaattactt aaaagcaacg tttacaaaat
      481 ctcttcgttt caactttaaa ttgctgtcat gtcgagcatt tctccacgct atactttgga
      541 tttgatggaa tcacccgttt ccattaaatc gcctgccggt gttaccgacc gagaagctca
      601 cgaccgcttt gaacgttctc tggatctttt tcatattggt gtcaactttg aagaagaagg
      661 tcaacccgag aaagccatcg cttttttttc ggagtcgatt gatgcaaatc cgaatatgag
      721 tctggcactt ttacgtagag gtaaactctt tttggaatca ggaatgtaca acgaggccct
      781 ggccgatttt ttcagttatc gcaatcagca gccccaatgt ccaagagtac tactctacct
      841 ttcgcgatta tctgcggtat tgtatgagga gaccaaagac gaattccata aaaacaatgc
      901 cgcccgttac ctcgattggg ctgtgcagaa gaatctgcat tattcaatgt tcaggattga
      961 tgagaattaa cgctctcgta attcatttgc tttattttct tcaaaccctc aatccaatcc
     1021 ttggctttat ccacatcgtt aaacattctc gtaggaatgg cgggtttgtt tacacggata
     1081 taaaagtttg caagtagtcg caatggcaat gaattgacca caagtgcatc ggcaatccgg
     1141 tattccagaa aaaatttttc agcataacac gagcgcgttt ctggtgctgc ataattcagt
     1201 ttgcgcagat caacaataca gtaataaggc ttgcgttcgg caagttcaat ggctgtgctt
     1261 aacaaatcac ggaatccttc caattccata tcaaccggtt ttgcatcgac catcattaca
     1321 tcgtcgttgt aacacaatat tgcaagaggt gtatccagtg ttttcatttg ttgttatcag
     1381 ggtattttgg ctccaatcaa tgtcacatcg tcaacctgat caagttctgc tttccaggct
     1441 tcgaatcgct cgatgatctg ctccttttgc acgatagcag gctgcttact gacttccatc
     1501 aataatttac gaaggcgcga aaaaagaaat ttctttccca gctctccgcc aaactgatcc
     1561 gaaaaaccat cgctgaataa aaaaagctga tcgcctttct ccatttgtat cctgtttgtg
     1621 gtaaacggtt ttaccggccc aatatgccaa cctattggct gattatctcc cttgagaata
     1681 gtaaacgatg ttccgcgaac aaatgcaaac atcgtattgg cacaagcata cctcaaaaca
     1741 cgtgtgttaa gatcaagctc aagaaaggtg agctccattc cgtcacgcat tactctctgc
     1801 tcctcttccc gctgtttgaa taagcgggta attccgctat ttaactcatc caaaaccaaa
     1861 gcaggttcgc gaatggaacg ttcaactaca atgtcattca gcaaggtaat accgatcatc
     1921 gtcataaagg cgcccggaat tccatgcccg gtacaatcaa cacacgctgc ccagatttta
     1981 tcatcctcac ggtgaaacca gaaaaaatct ccgcctaccg attctttcgg ccggaaaaga
     2041 atgaatgatt cgggcaatgc agattctatt cccttatcaa aacgcatcaa tgcgttctga
     2101 atccgacgag cgtaattcat cgaatcggta atctccttgt tcttggctcc caattgcaga
     2161 ttctgattct ccacaataag agaataccga agtaattcat tcctgtaaaa aaacaacagc
     2221 agaaatgagc taacaaaaag gataaccata ttaatcacat agattacctt gatgttcgga
     2281 ttctgaaaaa ccggttcaaa tgatgcgtat cccagtttgg aaataagaaa cccaatcaga
     2341 ctcagggtac tcattactat aatttcacgc agcgaatcga aaaacacaag cggacaaaca
     2401 cacaacaata tattcaggta ttcaattccg gattccggtc ccaataaaaa cgaaacgccg
     2461 ttaaaaaaca ccagaaagaa ggtcagacag aaccaccgtg caatacggta atacctcagg
     2521 gaattaaata cgagtgttaa aacaagaata agttgtccgc tcaggttggt gacaagaaaa
     2581 acaaccggac ttccctttgc gagattaatg accaacaaaa aaagaaaaca ggctagtcca
     2641 acaagtgcca ttatattcag caagcgcact tttctccctt cgtttggaga aagttcggca
     2701 tgaataccat tcgacatcag gcgttgcaac attgttcagg gcagtgataa aaccagaaca
     2761 atataataaa aattgaactg tccggtttgg atcagttgat tggttttgcg tggagatcgt
     2821 atggtccggc agactcaacc ttcacctgaa caaaatctcc aacacgtaga aaatcagcat
     2881 caatgatcac ctcattatcc acttcaggtg agtcgaattc tgtacgacca atgtaatgtt
     2941 gaccttcttt tcggtcgatt aacaccttga gtgtttgtcc taccttttct tcattgattc
     3001 ccgcagaaat acccaattga agctccatta tttcattggc gcgtcgttgt ttttcttcct
     3061 gaggaacgtc atcgtttaac ttataggcac ccgtattttc ctcgtgcgaa taagtaaaca
     3121 cacccaaacg atcgaagcgg gattgtccca cccattcgag catttgctga tgatcatcca
     3181 atgtttcgcc gggataaccg gaaataaggg tagtacgaat ggcaattccg ggaactttat
     3241 cgcggatttt gttcaccaga tcgatggtct tttgctttgt tgttccgcgt ttcatgcttt
     3301 tgagaaccgg atcagaaata tgctgcaatg gcatatcgag ataattgcaa accgattctc
     3361 gttcacgaat aacatccagt acgtcctcag gaaatccgga tggaaaagcg tagtgcaaac
     3421 gaatccagtc aataccctcc acatcactca agcgacgcat taattccgcc agcttacgtt
     3481 ctttgtacag atcaagtccg tagtaagtag aatcctgagc gatcagcaac aattcgcggg
     3541 ttccgttttt tgccagtttc tttgcacgat caaccaattc ttccataggt gtagaaacat
     3601 gtccgccacg catcaaagga attgcacaaa aggaacaagg tctgtcgcaa ccttctgcaa
     3661 ttttgaaata agcgtaatgc gaaggtgtgg tgagtaatcg ttcgccaaca agctcctttt
     3721 tgtaatcggc tttcagcgtt ttcagcaaac gcggaagatc acgtgtacca aaataagcat
     3781 caacttctgg gatctccttc tccagttccg gtttgtagcg ttcactcaaa caccccgtta
     3841 catacacttt ttcaacgagc ccttttttct tagcctctgt aaactgaaga attgtatcga
     3901 tactttcctg cttggcatta tcaataaatc cgcaggtgtt aataatcacg attgaagcat
     3961 catctgcgtt gctttcgtgc tccacatcaa aagcattggc ctttaactgc cccatcagca
     4021 cttccgaatc aaagaggttc ttggaacaac caagcgttac aacattaact ttattcttct
     4081 tgagagtacg cgttttcatg ctgcaaatat ccgaatcttt ttcccattct ctcggtattg
     4141 gtgtgctttt ggactatttt tgcagcttat ctcgcacatg gaattcaaaa tcatttccga
     4201 tttctatcca accggcgatc aaccggaggc tattcgtcaa ctctcagaag gtttacgccg
     4261 gggtgaacct gcacagactt tgctgggtgt aacaggttcg ggaaagacgt ttaccattgc
     4321 caatgtggta caggaagtga aacgtcctac actggtactt tcccataaca aaacactggc
     4381 agcacagttg tatgcagagt tcaaacaatt ctttccggat aatgccgtgg agtattttgt
     4441 aagttattac gactattacc aacctgaagc atatattcca gttacagata cctatatcga
     4501 aaaagatctg gcaatcaatg acgagattga aaaacttcgt cttgctgcca cttccgcatt
     4561 gctttccggc agaaatgatg taattgttat ttcgtcagta tcctgcattt acggtatggg
     4621 aaatcctgct gaattcagta agggaaaagt aaccatcaaa aagggacagg tcattgcccg
     4681 caatcagttt ttgcataaac tggtagatgg tttgtattcc agaacagaag cagatttcaa
     4741 cagaggtaat ttcagagtta aaggagatac catcgatatc tggcttgcct atgccgatca
     4801 tgcattacgg gttgtatttt ggggtgatga aatagaaagc attgaaacga tcgatccgat
     4861 gaattaccgg acgattgaaa aatttgaagt cttcaacatt tttcctgcaa atattttcgt
     4921 gaccagtcgc gatgtaatca aaaccgccac ctgggagatt cagcaggaca tggtaaagca
     4981 gatggaatac ctgaaggaaa tcggaaaaca catcgaagcc aaacgtcttg aggacagagt
     5041 cacttttgat ctggaaatga ttcgcgaatt gggatattgt tccgggatcg aaaactattc
     5101 acggtatttc gaccgccgta atcccggttc tcgtcccttc tgcctgctcg attatttccc
     5161 gaaagatttt ctgatggtga ttgatgaaag tcacgtaacc gttccgcaga tacgtgccat
     5221 gtatggagga gatcgttcgc gtaaggtgaa tttggttgaa tacggatttc gcttaccggc
     5281 tgcattggat aatcgtccgc ttaaatttga agaattcgaa actctggtgc aacaaattat
     5341 ttatgtgagc gcaactcctg ccgaatacga acttgaaaaa tcaggcgggg tggtggttga
     5401 acaacttatt cgtccaacag gcttactcga tcccgttata aatgttcgtc caagcggcaa
     5461 tcaggtagat gatctgctgg aagaaattgc agtggtaacc gaacgcgacg aacgtgtgct
     5521 ggtaaccact cttaccaagc ggatggctga ggaattggcg aaataccttg aacgaatggg
     5581 tatccggtgc aggtacattc actctgatgt ggatacactt gaacgagtgg aaattcttca
     5641 tgatctgcga aagggaatgt ttgatgtatt gattggtgtt aaccttctgc gggaaggatt
     5701 ggatttaccg gaggtgtcct tggtagctat tttggatgcg gacaaagaag gatttttgcg
     5761 ttcagagcgc tcccttgtgc agacagtagg aagagcggca cgaaatgtga atggaagagt
     5821 gatcatgtat gccgacaaaa tcacagattc catgcgcaag accatagagg aaaccaatcg
     5881 tcgcagagaa aaacagatac tgtacaatac cgagcatggc attactccta ctcagatcgt
     5941 aaaatcactg gaatcgcctc tcgcctttgg cagaacacgt ccggaagata aagtaatgcc
     6001 ctattccgga gaggaaatca ttcattctgc tgcagatcct gtgatgcaat accttaccaa
     6061 ggacgagtta ttgctccaca tcgaaaaggt gaaaaaaacc atggaaggcg aagcgaaaca
     6121 ggagaatttc attgaagctg cgcgtttgcg tgatgaattg ttcgggttaa tggaattatt
     6181 aaaaacaaaa taatatgcgt gttttcctat tcttcattct gattggtatt ttaccagcct
     6241 gcaagttgaa aaaacaaagc aaggacaaaa cagaagcaac aactactatg caaaatcacc
     6301 cggaagtaga aaccattacc gattatcagc gaccttccaa tcctgcatcg ttaactattg
     6361 taggtgcaaa aattgaaggt gatctgttgc atattgatgt cagttattcc ggtggttgca
     6421 ctgaacattc cttccgcctt attttttctg gcaactataa aaaatccatg cccccacaag
     6481 cttcagtgta tctcgaacac gatgcaaagg atgacacctg cagatcattg gtcgaaaaaa
     6541 gtctgatttt taatatccgg aaaatgcgct ttccacctga aaagcccgga acgctgatca
     6601 tttctattga aggttggaat gaacaactaa aatacgttta ttaacttttt taactgatct
     6661 gttttcttta aattgcagag aacaaaaacc gaatctgcat gatcacaaac aggacagtcc
     6721 gccaattgac tttaattgtt gttggcatct tagccacttt ttccatcggt ggaggtatta
     6781 ttgcgctgct ggctcacaac tgggatgatt ttccaaaggt tgtcaggatc attctaagtt
     6841 ttattcctgc tattttgggt attgctactt ttttatattc cgaatcgaag taccgcgatt
     6901 cacttacctg ggcagaatgt tcatctgttt ttcttgcatt gatgattggg tctaccattg
     6961 gcctggtggc gcaggtgtac aacatgggtg gttcatttga agatttcctt tttgtgtggc
     7021 tggttatgat tcttccaatt atctatctgg gaaattcatc ggcaacagct atcttttaca
     7081 tcggactttc acttacctgg ttatacctca cctacctgaa ttccttccgt ttctttttcg
     7141 gatttggctc cggcgcagga aacgatgtgt tgtggttctg gatattattc cttggtgttg
     7201 ttcctcacct gcgctatcat cttcagccgg gtagaatttc tttcagaggt gtaacgctgg
     7261 gttggtccgt tgcaattgca ttgtgtatgg ctgcttttat aggatttagc ggacaccgtc
     7321 tcatcaattc aacactgctc atcactcttg tgtattatat tggaaaagaa tatttcggca
     7381 atggaaatag cgcctggcaa cgtccgttcc aatccatctc catactatcc attttcctta
     7441 tgacccttat ttattcaggt catggtttat tgcgtttcac cttggaaaga gacggataca
     7501 ttcagtccta ttatggcgca ttcgatagag aatggcaggg agaaggaata cctgcaacgc
     7561 cctattttta ttacttcaat cttgttctgc tgtttgccat ggcatttgga gcaggttatt
     7621 ttttctggcg cgaaaaagaa agaaattccg attacaacaa agccatcctg gtttttccgg
     7681 gaatgatttt acttgcgata ctacttcagc tttttgaatt tgaaacaaca accaggattc
     7741 tcatcaatct ttatttattg ttcatggcat ggtggtacct ccgcgaagga tggagaatgc
     7801 gactcggctg gcttgtgttt tttgcggcac ttgttttctc tacaacaatg attggaaggt
     7861 atttcgatat ggatattccc ttctggatta aaggcatgat gtatcttgga ttgggagcag
     7921 gattgctttg gttcaataaa atttattcgg aggaaaaagc atgaacagaa aattcattct
     7981 attgattgtt cttggcttat tgctggcagt acctattgga atggtgattc gcaatgagat
     8041 gctgctggca gatgagggca gcatttacaa attcacgctg caacccattg atccttccga
     8101 cccagtccgt ggaaaatata taattctgcg ctacaatacc atgatcgaac tggacgaacc
     8161 cattcagttg caaaacggag aacccatcta tgtggaagtg aaaaaagatg aaaatggatg
     8221 ggcttatttc agcaatgtta gcactactcc acctgatcat tcggcttatt tcaaaacaac
     8281 agtcaattat ggttatgaaa gaaccgtcaa catcaatgtt ccattcgata aatacttcct
     8341 caacgaaaac cttgcaccat tggctgaatc ttcttacgca gaacttaccc gtcacggcga
     8401 ggaattttat gcggaagtta gtattactaa tggagaagca cttttgcgcg gagtgtatta
     8461 catgaatcga ccactgatcg atcatttgcg cgaaatgcag gtagatcagt aaagatcaaa
     8521 cagggaatgt acccccggag tacgttccac tttaaatgcc tcagcataca tcacctgaat
     8581 cggtcgcccg aatgcaatgg cttttgcttt gaggtattcc ataaactctt tgctgctaat
     8641 gggagtctgt ggttctccgg atgaatcttt tccggtatgg aattgagaag aatatgcaag
     8701 aatactttcc attttctttt cccaatacgg agtgatatct attacaagat cataatcctc
     8761 tatccggtct tgaatgtaat ggtacacagc cttcggtctc caggctgatt gtgctccacc
     8821 accgaactgc gattcgatct tgacaagccc tgaaagaaaa caagcacgtg aaaccagctc
     8881 agctgcacgc gaatgatcag gatggcgatc gctgagtgcg ggtgcaagaa caatctcagg
     8941 acggtacttc cggatcactt caatcacctt gagcagtgag gcttcattga tttcgaagaa
     9001 accatctgcc attccaaggt tttctcttac atcaatacca agaatggcag atgcgttttg
     9061 tgcttcttca cttcgtagat ccatgtttcc acgcgatccc agttcgccgc gggtgagatc
     9121 gattattcct gtggttttgc cttgtgcctt gtgcagcaga agtgttccgg aggcggacaa
     9181 ttccacatca tcagggtgag ccgcaaaggc aaggaggtcg atcttcatca tcagggagtt
     9241 tgggcaaagt taagaatagt gattgaaaaa ggccgcacaa gggcggcctc tatcatattt
     9301 tgaatgcttt atttcttgat ccactcgatg atggacggat catcaggtaa tgtacgtgga
     9361 tgaatgttct taacgtattt accggtttca tcaatcagga ttttatgaaa attccatttt
     9421 acatcccaat cctcaactcc attttccacc ttggtagtaa agaatttata tatggagcct
     9481 gatcggaacc tgtaaccgat attttcgaca ttaccggaaa agtaacacca tagtttttgg
     9541 tacaaaatcc tttgatctgc tcatcacttc cgggctcctg tgcaccgaaa ttattggcag
     9601 gaaaagcaat gatcgtaaat ttttctcctc cgtaggtttt gtacacttcc tccagttgct
     9661 tgtactgtgg agtgagtccg cactcgctgg caacgtttac aatcataatc tttttccctt
     9721 tcaaggaagc aaagtcgaag ggttttccat caatatcgtt cactttaaaa tcgtaaatcg
     9781 ttttcatagt tgctaagctt gttgctgatg ttgttttctg cttttttgat ttctttttgc
     9841 catcctgctg acaagcatac acagcagaca aagaaagaac caatgaggta ataatgaaaa
     9901 tggaac
//
LOCUS       DAOMYG010000053         9903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2278_length_9903_cov_9.202275, whole genome shotgun sequence.
ACCESSION   DAOMYG010000053 DAOMYG010000000
VERSION     DAOMYG010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 9903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9903
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2278_length_9903_cov_9.202275"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="PK610_02870"
     CDS             <1..1306
                     /locus_tag="PK610_02870"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na-translocating system protein MpsB"
                     /protein_id="HRJ34896.1"
                     /translation="ALKSDVHFARNSHSLFRGWLLSMTIGFWSAFRLFISLFKPSVSP
                     VAAYSFSHMDGASGLQIDADGTTEGELKVGFTHEEMALRVMNTLRSIGLTGNFSRLVY
                     VIGHGASSVNNPYYAGYDCGACCGRPGAVNARVFSYMANKPEVRLILAEKGIHIPETT
                     IFIGAMHDTTRDEMEYYDLHLLSAEQVQSHENCQKVFSKALQLNADERSFRFDNVKSA
                     SGSSGLHAEIKKRSVSFFEPRPEWNHTANALTIVGRSHLYKHMYLDKRPFINSYDYRE
                     DPEGKYLLSILSAAIPVCGGINLEYYFSRVDQQKLGAGTKLPHNVVGLFAVNNGVEGD
                     LRPGLPSQMIEIHDPVRILFVIEHHPEVVFRVISSNAGIYEWIKNEWVHLTVKDPSTG
                     KTYRYADGELVLYNSLQKQLKKARDKAQLEKSYAKNEVVLIN"
     gene            1312..3183
                     /locus_tag="PK610_02875"
     CDS             1312..3183
                     /locus_tag="PK610_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRJ34897.1"
                     /translation="MYHIIHLLVLLPFSGFLLSLLFNGTRERSIAMVAYVIMFVSMSL
                     SVVFSIYWLTIGSPVFSFADGSLYAQNGFNFFLDFYFDGIAATYLVVGNIITFMVIAF
                     SRHYMHRESGYKRFFNTILFFNTGYVITILAGNFETLFIGWEILGISSFLLIAFYRLR
                     YLPVRNALKVFTIYRIGDVGILLAMWMSHHLWSENITFQKLHNYELVHEHLSAHTGIG
                     VFVSLMILVAALAKSAQFPFSSWLPRAMEGPTPSSAIFYGSLSVHMGAFLLLRTHPFW
                     EHQVSVKILIVVIGLVTAVLASLMARVQSTIKGQIAYSSVSQIGIIFVEVGLGFETLA
                     LIHFAGNAFLRTYQLLISPSAVAYMIRDQFYHHQDTEQKSSASWKSKLNSTFYLLSLK
                     EWNLEIIVFNLFIKPLKKIRVLLGFLTVRNVAFVLFPLYIIGFILAYKNPAAITESRI
                     VIAHICAVLALLMVAKSFNERTSSRLAWMLVVFAHFFVDLAVTFNDHLDLKEATIYLS
                     GIIISGSIGLIALLRIRRIEHHGLGLNRFHGLVKEYPVTAFVFLLSALGVAGFPITTT
                     FFGEDLVLTHIHENQVLLAFFVSVTFIVNGLAVIRIYSRIFLGTHQRSVQHASDLTV"
     gene            3205..4800
                     /locus_tag="PK610_02880"
     CDS             3205..4800
                     /locus_tag="PK610_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRJ34898.1"
                     /translation="MSTTIDTNKKSLTLSAKFSDNWSSDLLAGFLVFLLALPLSLGIA
                     KASGFPASMGVLTAIVGGLLTSIFRVSPLTIKGPAAGLITICSGAIIEFGGAEQGWKI
                     ACAAIMVAALLQLVIALAKLGALSDFFPHSAVHGMLAAIGIIIIAKQIPVLLGDDPSM
                     YKGEGPIELLLDIPNFISHAHWHIAIVGIIGLIVMFGLPLIGIRLLKKVPAPMVVLML
                     TIPLGVFWHFRETEPAYSLVQIGDFWGSVGFNFDFSLISSFVFWKYVFMFLFVNSLES
                     LLTVKAIDDLDPYKRSSSYNGDLFGLGAGNFVSGLLGGLPMISEVVRSSANVGFGGRT
                     KWSNVFHGTFLLLAMIFLIPFIEMIPNAALAAMLIFAGFRLAAPKEFIHTYQVGKEQL
                     AIFLVTIIVTLMEDLLLGVASGIIVKILFHLFNGVSIRSLFVAHFDKKETEDEIYIKI
                     KESAIFSNLIGFKKMFMSIQNDKKLVVDLSETNLVDHSFLSFINHYKNESIEHNRPMS
                     IIGLENHKAFSSHPLATMKRKVK"
     gene            complement(4905..5378)
                     /locus_tag="PK610_02885"
     CDS             complement(4905..5378)
                     /locus_tag="PK610_02885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRJ34899.1"
                     /translation="MKRPWNIPSLAVYSLATYEKGKVNMNICTYVSAVSMKPKRYAVA
                     VFHGTKTLKNLKSNKNAVLQLLGEEQYPLIRMLGKTSGKQYDKSEWLSKRNRTIQWKG
                     HTVLKDASAYIQLSYHSEINAGDHHLYIFDVIAYKSNSEHILTTEILSQKKLIRI"
     gene            complement(5660..6070)
                     /locus_tag="PK610_02890"
     CDS             complement(5660..6070)
                     /locus_tag="PK610_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRJ34900.1"
                     /translation="MKTVKKTTRKAYGAIKYESVEHYHAAQTEDVKEILEQLRKCIHK
                     AVPHLTEKISYNMPAFCIDKVLVYYAANKSHVGFYPTTGPIIAFSKELEKFQTSKGAI
                     QFPYNEKLPIGLITKIVKFRKAEEEERVRNKKKK"
     gene            complement(6080..6430)
                     /locus_tag="PK610_02895"
     CDS             complement(6080..6430)
                     /locus_tag="PK610_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRJ34901.1"
                     /translation="MSLNVKDLQASKIFYEHLGFTTSGGDMKDNYLIMKNQNALIGLF
                     QGMFEKNILTFNPGWDESAKDLKEFDDVREIQQKLKAKGIVLDVEAPDTTKGPAYIML
                     KDPDGNEILIDQHR"
     gene            complement(6653..7666)
                     /locus_tag="PK610_02900"
     CDS             complement(6653..7666)
                     /locus_tag="PK610_02900"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRJ34902.1"
                     /translation="MVLVTGSTGLLGSHLLLDLVRHGKKVRALVRNREKIKNVELLFH
                     YYVPDSATEFLSTIEWFDGDVCDIFSLDDALDGITEVYHCAALVSFDPSDRRRMTEIN
                     DIGTANLVNACLDAQIRKLCHVSSTAALGRSKSNEMITEKTQWKNAPENTWYAITKYN
                     AEREVWRGIEEGLCAVIVNPSVILGPADWNSGSTAMFRQGSGGMKFYTEGGNAIVDVR
                     DVSAIMLQLMESQIEEERFLVTGENIRFRDLFNKIARAFEKKPSSIKATSFMTGFAWR
                     WEKLASKLTGRKPLITRETARAAHAVYEYDSSKIIQALNYRFKNADEAIGNTVNFLNT
                     LKK"
     gene            7792..9081
                     /gene="tyrS"
                     /locus_tag="PK610_02905"
     CDS             7792..9081
                     /gene="tyrS"
                     /locus_tag="PK610_02905"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRJ34903.1"
                     /translation="MKNFIEELRWRGMLHDVMPGTEELLNSGKPVKAYIGFDPTADSL
                     GVGNLVQVMTLVHYQRCGHQPIALVGGATGLVGDPSGKSQERNLLTAELLQHNLNCQR
                     AQLEKFLDFSGTNAASIVNNYDWFSSFSFLDFIRDVGKHITVNYMMAKDSVKKRITGE
                     EREGMSFTEFTYQLIQGYDFFHLWKNHGVLLQMGGSDQWGNITTGTELIRRMDGGEAF
                     ALTTPLIKKADGTKFGKTESGNIWLDKKRTSAYQFYQFWLNAADADAENYIKIFSIKD
                     KEEIEAIIAEHRAAPHERKLQKELAREITVRVHSDADYETALEASAILFGKATSENLR
                     KLSEDDFLSVFEGVPQGKIPRAELHTGVDLVTLLGEKSGFLKSRGEARRAIEENSIAL
                     NKDKVTMEQIVSDADLISGKYLLLQRGKKNYFLLIAE"
     gene            complement(9082..>9903)
                     /locus_tag="PK610_02910"
     CDS             complement(9082..>9903)
                     /locus_tag="PK610_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ34904.1"
                     /translation="GGVHTGLEPGNYTYTATDPRGCSVTETVTITEPDELLANISTEN
                     VSCHSAGDGEVAITITGGTPGYHITFNGSTGTNLTYSSLNPGVYTIEAEDANGCVTTQ
                     QSVTISEPNVLTSTGSSTPETSLGDNDGTASVSASGGTAPYTFLWNDNSTQTTATANG
                     LPSGTYSVLITDANGCTHSETVFVSHTTGTEEIEQKLTVTVAPNPVNEWMEITISAQE
                     QKASIQMTDITGRTVYQSSANGNHRINCAEFGAGNYLLLVQTGNLSQTIRVVIAR"
BASE COUNT         2645 a         1897 c         2116 g         3245 t
ORIGIN      
        1 ggcattaaaa agcgatgttc attttgcgcg gaattctcac tcattattcc ggggatggtt
       61 gctttccatg acaattggat tctggtcggc atttcgcctt tttattagtt tgtttaaacc
      121 ttccgttagt cctgtcgctg cttattcatt ttctcatatg gacggcgcat caggattgca
      181 aattgatgca gatggtacca cagagggtga attgaaggtg ggatttaccc atgaggaaat
      241 ggccttgaga gtgatgaaca ccttgcggag tattggactg accggaaatt tttcaagact
      301 cgtttatgta atcggtcatg gagcaagttc agtcaacaat ccctattacg ccggatatga
      361 ttgtggtgca tgttgtggaa gaccgggtgc ggtaaatgca agggtgtttt cttatatggc
      421 aaacaaacct gaagtgcgtt tgattttggc ggagaaggga attcatattc cggagaccac
      481 aatttttatt ggcgcaatgc acgatactac ccgtgatgaa atggagtact acgatttgca
      541 tttgttgtcg gctgaacagg ttcaatcgca tgaaaattgc caaaaggtgt tttcaaaagc
      601 attgcaactg aatgccgatg agagaagttt tcggtttgat aatgtaaaaa gcgcgagcgg
      661 tagttcaggt ttgcatgccg aaataaaaaa gcgttcagta tctttttttg aacctcgtcc
      721 cgaatggaat cataccgcaa atgcattgac aattgttggg agatcacatt tgtataaaca
      781 catgtacctc gacaaacgcc ctttcatcaa ttcatatgat tatcgtgaag atccggaagg
      841 aaagtatttg ttgtccattt tgtcggctgc tattccggtt tgtggtggta ttaatcttga
      901 atattatttt tcaagagtag atcagcaaaa acttggtgcg ggtacaaaac taccgcataa
      961 tgtggttgga ctgtttgctg taaacaatgg tgttgaagga gatctgcgtc ctggcttgcc
     1021 aagtcaaatg attgaaattc acgatccggt tcgaattctt tttgtcattg aacatcatcc
     1081 ggaagttgta tttcgtgtga taagttcaaa cgccggcatt tacgaatgga taaaaaatga
     1141 atgggttcat ctcactgtta aagatccctc aaccggaaaa acatacaggt atgctgatgg
     1201 ggaattggtc ctctataact ctttgcagaa acagctaaaa aaggccaggg acaaagctca
     1261 actggaaaag agctacgcaa aaaatgaagt tgtacttata aattgatcac tatgtaccac
     1321 atcatacatt tactcgtcct tttaccattc tccggattcc tgctttcatt attgtttaat
     1381 ggaaccagag aacggtctat tgcaatggtc gcttatgtga tcatgtttgt ctccatgtcg
     1441 ctttcagtag ttttttccat ctattggctc actattggtt ctccagtttt cagttttgct
     1501 gatggaagtc tctatgcgca aaatggattt aatttcttcc tcgattttta ttttgacgga
     1561 attgcagcga cttatttagt tgtaggtaat atcatcactt ttatggtgat tgcttttagc
     1621 cgtcattata tgcatcgcga atcaggctac aagcggtttt ttaataccat cctctttttc
     1681 aataccggtt atgtaataac aattcttgcc ggaaactttg agacgctatt tattggttgg
     1741 gaaattctag ggatttcatc ctttctgtta attgcatttt atcgtttgcg ctatctcccg
     1801 gttcgtaatg cattaaaggt gtttaccatc tatcgtattg gtgatgtagg gatactactg
     1861 gccatgtgga tgagtcatca tttatggagc gagaatatta cttttcaaaa gctccataat
     1921 tatgaattgg tacatgagca tttgtcggcg cataccggaa taggcgtttt tgtttcgctg
     1981 atgatccttg ttgctgcttt ggcgaagtct gcgcagtttc cattttcatc atggttacca
     2041 cgggcaatgg aagggccaac tccgtccagt gcaatttttt atggatcact ttctgtgcac
     2101 atgggagcgt ttctgttatt gcggacacat cctttttggg aacaccaggt ttcagtaaaa
     2161 attctgattg tagtaattgg tttggttaca gcagtgcttg cttctttaat ggcgcgtgtt
     2221 cagtcaacga ttaagggaca aattgcttac agttcagttt cacaaatcgg aatcatattt
     2281 gtcgaagtag gtcttggatt tgaaacactt gccttaattc atttcgccgg taatgcattt
     2341 ttaagaactt atcagctctt gatttctccc tccgcagtag cgtacatgat tcgggatcag
     2401 ttttatcatc atcaggatac agaacaaaaa agttccgcgt cctggaaatc aaagctgaat
     2461 tccacttttt accttctttc cctaaaggag tggaacctgg aaatcatagt ttttaatctt
     2521 tttataaaac cacttaaaaa gatcagagtg ctattaggat tccttacggt cagaaatgtt
     2581 gcattcgttt tatttccgct ctacattatt ggatttattc ttgcctacaa aaatccggct
     2641 gcaataacag aaagcagaat tgtcattgcg catatctgtg cagtactggc attgctgatg
     2701 gtggcaaaat cattcaatga aagaaccagc tctcgtttag catggatgtt ggtagtgttt
     2761 gcccattttt ttgtggatct ggcagtcacc ttcaacgatc atcttgattt aaaagaagct
     2821 actatttatt tgagcggaat tattatttca ggatcaatcg gattgatagc gcttttaaga
     2881 atcaggcgaa ttgaacatca tggacttgga ttaaaccgtt tccatggatt ggtcaaagaa
     2941 tatccggtaa ctgcatttgt gtttctgctt tctgcattag gtgtggcagg ttttccgatt
     3001 acaacaacat tttttggaga agacctggtt ctgactcata tccacgagaa tcaggtattg
     3061 cttgcgtttt ttgtttcagt aacatttatc gttaacggat tggcagtaat caggatttac
     3121 tccagaatat ttttaggtac acatcaaaga agcgttcagc acgcatcaga tttaacagta
     3181 taaattttca tcaaaaacat tagtatgagc acaacaatag atacaaataa aaaaagcctc
     3241 acgctttccg ctaagttcag tgacaattgg agttctgatc tacttgccgg ttttctggta
     3301 tttttacttg cactccctct tagtttgggt atagcaaaag ccagtggttt tccggcctcg
     3361 atgggggtgc ttactgcaat agttggtggt ctattaacca gtatttttcg ggtttcacca
     3421 ttaacaatta aaggtcccgc ggcaggattg attacaattt gttcaggagc aattattgaa
     3481 ttcggaggtg ctgagcaagg ttggaaaata gcctgtgctg caattatggt ggctgcgcta
     3541 ttgcaattgg taatcgcgtt ggcaaaactt ggagcgctaa gcgatttttt tcctcattcc
     3601 gcagttcatg gtatgcttgc tgcaattgga ataatcatta ttgcaaaaca aattccggtg
     3661 ttgcttggtg atgatccttc aatgtacaag ggcgaaggtc cgatcgaatt attgctcgac
     3721 attccgaact ttatttcaca tgctcactgg cacattgcga ttgtaggcat tatcggattg
     3781 atcgttatgt tcggattgcc gctaatcgga attcgtttgc tcaaaaaagt tcctgcacca
     3841 atggtagttt taatgctaac cattcctctc ggagttttct ggcatttccg tgaaacagaa
     3901 cccgcgtact cactcgttca aattggagat ttttggggtt cagtcggatt caatttcgat
     3961 ttttcactga tctcttcatt tgttttctgg aagtatgtat ttatgttttt gtttgtaaac
     4021 agcctggagt ccctacttac agtaaaagca atcgatgatt tggatcctta taaacgatcg
     4081 tccagttaca acggtgatct tttcggtttg ggtgccggta attttgtttc gggtttgttg
     4141 ggtggattgc cgatgatttc ggaggtggtt cgtagctctg ccaatgttgg tttcggcgga
     4201 agaaccaaat ggtcgaatgt ttttcacggc acatttttgt tattggcaat gatttttctc
     4261 atccctttca tagaaatgat tcccaatgcg gcactagctg ctatgctcat ttttgcaggt
     4321 tttcgtcttg ctgcaccaaa agagtttatt cacacttacc aggtcggcaa agaacaactt
     4381 gcaatttttc ttgtaacaat aattgtaacc ttaatggagg atcttttatt aggtgttgca
     4441 agtggaatta ttgtaaaaat tctctttcat ctgtttaatg gtgtttctat cagaagtctt
     4501 tttgtagcac acttcgataa aaaggaaaca gaggatgaga tctacattaa gatcaaagag
     4561 tcagcgattt tttcgaacct gattggattt aagaaaatgt tcatgtccat tcagaacgat
     4621 aaaaaactgg tagttgatct ttccgaaaca aatctcgtcg atcattcttt cttgtctttt
     4681 attaatcatt ataaaaacga atccatcgaa cataaccggc caatgagtat tatcggactg
     4741 gaaaaccata aagcattttc cagtcaccca ttggccacta tgaaacgcaa agtaaaataa
     4801 acaagatggg ggtgttggca ggaattatga tcgtgttgat ttggttttgg tttggttgat
     4861 cgatctcaca gcttgcaatc atgttcaggt caacaccctt tttattaaat tctgataagc
     4921 tttttttgag agaggatttc agtcgtaaga atgtgttctg aattactctt gtaggcaatc
     4981 acatcgaaaa tgtagaggtg atgatctcct gcatttattt cggagtgata actcaattga
     5041 atataagccg atgcatcttt aagtacggta tgtcctttcc attgtattgt acggtttcgc
     5101 tttgaaagcc attccgattt atcatattgt ttgccggatg tttttcccaa catacgaatc
     5161 aatgggtatt gttcttctcc taaaagttgg agcaccgcat ttttattcga tttcaggttt
     5221 ttaagtgttt tagtaccatg aaaaacagca acagcatacc gttttggctt catgcttacc
     5281 gcacttacat aggtgcaaat attcatgttg acttttcctt tttcgtatgt agccaaacta
     5341 tacactgcca aagacgggat gttccagggt cgtttcatga taatctaaca gactgacctt
     5401 tattttgttt cttgttgata atcaggtaat tttattaaga atgattcaaa ataccatttt
     5461 tagggtattg ggaaattccg actgttgatg cagttttgat gtatggaaat ccgccggcct
     5521 tttacgggaa ttgaacttca acaacaattg gaggagaatt gctgtatcct aaagcagctg
     5581 tttgcggata agaaaaaaaa gtgctgcaaa aaatacaaga aaagcaagcg ctgcggatct
     5641 tgtccgaaag gatgaggaat tattttttct ttttattacg gactctttct tcttcctctg
     5701 ctttccggaa ctttacaatt ttagttatca atccgatcgg aagtttttca ttgtagggaa
     5761 actgtatggc tccttttgat gtttgaaatt tttcaagctc tttgctgaag gcaatgatcg
     5821 ggcctgttgt tgggtaaaat ccgacatgcg atttgtttgc agcgtaataa accaaaactt
     5881 tgtctataca aaacgccggc atattgtagc ttattttttc tgtcagatga ggtactgctt
     5941 tatgaataca cttccgaagt tgttccagaa tttcttttac atcctccgtt tgagcagcat
     6001 ggtagtgttc taccgactca tactttattg ctccgtaggc tttgcgtgtg gtttttttta
     6061 ccgtcttcat tagaacaaat taacgatgtt gatcaattaa aatttcattt ccatcaggat
     6121 cttttaacat aatatatgcc ggacctttcg ttgtgtcggg agcttcaaca tcgagtacta
     6181 ttcctttcgc tttcagtttt tgttgaattt cacgtacatc gtcaaattct ttcagatctt
     6241 ttgcgctttc atcccatccc ggattaaacg tgaggatgtt tttttcaaac atgccctgaa
     6301 acaaaccaat caatgcgttt tggtttttca tgatcagata gttgtctttc atatcgcctc
     6361 cactggtggt aaaacctaaa tgttcataga atatttttga ggcctgaaga tcctttacgt
     6421 tcaaactgat tgaaaaggct ccgagcggta aagtgctttc tgcaaccgga gtttgtttta
     6481 ctactgctgt atcacatgtt gatttgtcgc aactatgtgc gctcttgcaa aaaatagaat
     6541 tagtaaagaa tcccagaaaa taagaaccga tcagtaaaag aaaaatgata aaaatgtttt
     6601 tcatggttta tagtttaaat atgctatgtt gatctattta ttaatgcaca aattactttt
     6661 ttaaagtatt tagaaaattt accgtgttgc caatggcttc atctgcattc ttaaagcgat
     6721 aattcaaggc ctgaataatt ttactgctgt cgtactcata aaccgcatga gccgcacgtg
     6781 cagtttcgcg ggtgatgagt ggttttcttc cggtgagttt actcgctaat ttttcccatc
     6841 tccatgcaaa gccggtcatg aagcttgttg ctttaatgct tgatggtttt ttttcaaagg
     6901 cacgggcaat cttattgaag agatcgcgaa aacgaatatt ttctcctgta accagaaacc
     6961 gttcttcctc aatctgactt tccataagtt gaagcatgat tgccgaaaca tcacgcacgt
     7021 ctacaatagc attacctcct tctgtataga atttcattcc gcctgaaccc tgacgaaaca
     7081 ttgcagtgct gccggaattc cagtcggccg gtcctaatat tacactgggg ttaacaataa
     7141 cagcacataa gccttcttct atccctctcc acacttcccg ttccgcattg tatttggtaa
     7201 tggcgtacca ggtgttttcc ggtgcgtttt tccattgtgt tttttcagtg atcatttcat
     7261 tactcttcga tcggccaaga gctgctgtgg agctgacatg acaaagtttt ctaatttgtg
     7321 catccagaca tgcgttcaca agattggctg tgccgatgtc gtttatttct gtcattcgtc
     7381 ttctgtccga cggatcaaaa cttacaaggg ctgcacagtg gtacacctct gtaattccgt
     7441 ccagggcgtc atccaatgaa aaaatatcac aaacatcccc gtcaaaccat tcaatggttg
     7501 aaagaaattc agttgcacta tcaggaacgt aataatgaaa aagaagttca acgtttttga
     7561 tcttttcacg gtttctaacc agggcacgaa cttttttacc gtgacgaacc aggtccagca
     7621 aaagatgtga gcctaatagt ccggttgatc ctgttactaa aaccatgggg taaagatagt
     7681 attatagcct tttccatgct ttaggaaagc tcaagaaagg tggctattgt cttgattgat
     7741 aattccattg ctgcagtatt tttcgttctg cgaatacttt atctttgcag gatgaagaat
     7801 tttattgaag aactccgctg gagaggcatg cttcacgatg ttatgccggg aaccgaagaa
     7861 ttgcttaatt caggtaaacc cgttaaagca tatattggat tcgatcccac tgccgattcg
     7921 cttggggttg ggaatcttgt tcaggtcatg acactcgtac attaccaacg ttgtggtcac
     7981 caacccatcg ctttggttgg gggagctacc gggctggtgg gagatccttc aggaaaaagt
     8041 caggagcgga atcttcttac tgccgaattg cttcaacata acctcaattg tcagcgtgcg
     8101 cagcttgaaa agttccttga tttttcagga acaaatgctg ccagcatcgt gaataattac
     8161 gattggttca gttcatttag ttttctcgat tttattcgtg atgtgggaaa acatattacg
     8221 gtgaattaca tgatggcaaa agactcagtg aaaaagcgca tcacaggaga agagcgcgaa
     8281 gggatgtctt ttacagaatt cacatatcag cttattcagg ggtatgactt cttccacctt
     8341 tggaaaaacc atggtgtact attgcaaatg ggtggaagcg accaatgggg aaatattaca
     8401 acgggaacag agcttatccg aagaatggat ggaggagaag cttttgcgct tacaactccg
     8461 ctgattaaaa aagctgacgg aacaaagttt ggaaaaacgg aaagtggaaa tatctggctg
     8521 gataaaaagc gtacttctgc ttaccagttt tatcagtttt ggctgaatgc agcagatgca
     8581 gatgcagaaa actacatcaa aatattttca attaaggaca aagaagagat tgaagcaatt
     8641 attgctgaac accgtgccgc acctcatgag cgaaaactcc agaaggaatt agctcgggaa
     8701 ataactgtca gggttcactc tgatgccgat tatgaaactg ctcttgaagc ttcagctatt
     8761 ctttttggaa aagcgacatc tgagaatttg cgtaagctgt ctgaagatga ttttctttct
     8821 gttttcgaag gcgttcccca ggggaaaatt ccgagagcag aactgcatac cggagttgat
     8881 ctggtgacat tgctcggcga aaaaagcggt ttcctcaaat cacgaggcga ggcacgtcgc
     8941 gccattgagg aaaattcgat cgccttgaac aaggataaag ttacaatgga acaaatagtc
     9001 tccgatgctg atttaatcag cggaaagtat ttgttattgc aacgcggaaa gaaaaattat
     9061 tttcttctga tcgcagaata attatctggc aattacaacg cgtattgttt gactgagatt
     9121 gcctgtctga acaagtagca aataatttcc tgcaccaaat tcagcgcaat taatgcggtg
     9181 atttccattg gcagaacttt gatatactgt tcttcctgtt atgtctgtca tttgaatgga
     9241 cgctttttgt tcctgtgctg agattgttat ttccatccat tcatttacag gatttggcgc
     9301 aactgtaaca gtgagttttt gttcgatttc ttctgttcct gtggtatgac taacaaacac
     9361 tgtttctgaa tgcgtacatc cgttcgcatc cgtaatcagt acagaatatg taccactggg
     9421 aagtccgttc gctgttgctg ttgtttgtgt ggaattatca ttccaaagaa aagtgtaggg
     9481 cgctgttcct ccgctggcac ttacgcttgc tgttccgtca ttgtcgccca gactggtttc
     9541 cggcgtgctg ctgccggttg aggttagtac gttgggttcg ctgatggtta ccgattgctg
     9601 cgtggtgaca cacccatttg catcttctgc ttcgatggtg taaacacccg gattgagaga
     9661 agaataagtc agattggttc cggtagatcc gttaaacgta atatgataac ccggtgttcc
     9721 tcctgttatt gtgatcgcaa cttcaccgtc gccggcagag tggcagctta cattttcagt
     9781 ggaaatattc gccaagagtt catcaggctc ggtgatggta acagtttccg ttacactgca
     9841 cccgcgcgga tctgttgcgg tataggtata atttccgggt tcaagtccgg tgtgaactcc
     9901 tcc
//
LOCUS       DAOMYG010000054         9809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2307_length_9809_cov_8.627435, whole genome shotgun sequence.
ACCESSION   DAOMYG010000054 DAOMYG010000000
VERSION     DAOMYG010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9809
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2307_length_9809_cov_8.627435"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..502
                     /locus_tag="PK610_02915"
     CDS             134..502
                     /locus_tag="PK610_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRJ34905.1"
                     /translation="MAKIIGLGGIFFKSPDPAALRKWYCEVLGIKAEEWGTVFPFTEN
                     ESGYNVWSVMKSDTEYFSPSTSSFMINFRVDDLDVFIAELIKKNISLCGDPQMGEYGK
                     FAWILDPDGNKLELWQPPQS"
     gene            complement(556..1851)
                     /gene="nqrF"
                     /locus_tag="PK610_02920"
     CDS             complement(556..1851)
                     /gene="nqrF"
                     /locus_tag="PK610_02920"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HRJ34906.1"
                     /translation="MTQTLVMALLVFLGVTLLLVALLLYAKSKLVASGKVKIIINDDK
                     VLEVNAGSSLLSTLGNEKIFLPSACGGGGTCVQCKCQVVEGGGEILPTEVPHFSRKEV
                     KDHWRLGCQVKVKQDMKIHIHEEILGIKEWEATVVSNYNVATFIKEFIVEIPEDMDYK
                     AGGYIQIKIPACTVKYSEMDIAAHPQDHPGEPDKFRKHWDKFKMWDLVMKNNEEVVRA
                     YSMASYPAEGRKIMLNVRVATPPWDRAKNTWMNVNPGIASSYIFNLKPGDKAVISGPY
                     GEFFINESDAEMLYVGGGAGMAPMRSHLYELFKTIKTGRKVSYWYGGRSKEELFYIHY
                     FRDLEKDFPNFKFYLVLSEPLPEDNWVVKKDINDTAGDGFVGFVHQAVIDQYLSKHDS
                     PEDIEFYFCGPPMMNNAVTKMCDDWGVPKENVRFDDFGG"
     gene            complement(1872..2486)
                     /gene="nqrE"
                     /locus_tag="PK610_02925"
     CDS             complement(1872..2486)
                     /gene="nqrE"
                     /locus_tag="PK610_02925"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HRJ34907.1"
                     /translation="MELLNIFIKSIFIDNMIFAFFLGMCSYLAVSKTVKTAVGLGMAV
                     IFVLTMTVPLNYLIENYFLKPGALAWMGEEYKSIDLSFLSFIVFIAVVAAFVQLVEMS
                     VEKFLPALYGALGIFLPLIAVNCSILGGALFMQERQYPSIAHVTSFALGSGIGWFIAI
                     VLLAAIREKIRYSHVPAPLRGLGITFILTGLMGIAFMSFMGIKF"
     gene            complement(2506..3192)
                     /locus_tag="PK610_02930"
     CDS             complement(2506..3192)
                     /locus_tag="PK610_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HRJ34908.1"
                     /translation="MSEATAPAKSPSEGLFSKKNRKLITDPLNDSNPITVQVLGICSA
                     LAVTVKLDNSVVMAASVTAVTIMGNFFMSLMRNIVPNRIRIIVQLVVVAWLVIIVDQV
                     LKAYVYDVSKSLSVFVGLIITNCIIMGRIEAFALGNKPGPSILDGLGNGIGYGIILIF
                     LGIVRELFGSGKLWNAQVLPANWIYSMDNPDGWYVLNNFMIMPPMALILVGLYIWAQR
                     ARNTKLIEAH"
     gene            complement(3197..3934)
                     /gene="nqrC"
                     /locus_tag="PK610_02935"
     CDS             complement(3197..3934)
                     /gene="nqrC"
                     /locus_tag="PK610_02935"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HRJ34909.1"
                     /translation="MAINKDSNAYTIIFAIAMCVVVGTSLVIVSQTLKPVQEKNVMND
                     KKQNILAAIQVEATLQDAGTKFEQYVKDAVILDANSQAVEVKTDVIAEALTINALSEF
                     KDKPATDRRYPLFICEKDGSRFFVMPVGGQGLWAGVWGYVAVGEDLNTIAGASFDHKS
                     ETPGLGAEIATPFFETQFTGKKIHDDNGEFVSIRVVKPGGPKSDHAVDGISGGTFTSV
                     GVDEMLMRCMSIYSKYFKTMSKSTALN"
     gene            complement(3937..5121)
                     /locus_tag="PK610_02940"
     CDS             complement(3937..5121)
                     /locus_tag="PK610_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HRJ34910.1"
                     /translation="MKFLENMMKKMEPSFKKGGKFEKLHPLYDGFATFAFTPNHATHS
                     GAHIRDSIDLKRTMMTVIIALIPCLVFGMWNVGYWHYSALGIPATMMQEFVYGLIKVL
                     PLVVVSYGVGLGIEFAFCIAKGHAIQEGFLVSGMLIPLVMPADVPLWMVAVATAFSVV
                     IAKEVFGGTGMNIVNVALTARAFLFFAYPTFMAGEVWISGDKTQFVDGTTGATNLGHL
                     AGLVTNPETKDVVMNEKVADLWTTFIGIEPGSVGETSGLAILIGAGILILTGIGSFRI
                     MASMFAGGFLMQTIFNAWGANPYMEVDPMHQLMMGGFMFGMVFMVTDPVTAAQTPTGK
                     IIYGVLAGMLSILVRVVNPAFPEGVMMAILLSNVFAPTIDHYVVQANIKRRLNRAKLK
                     TA"
     gene            complement(5165..6529)
                     /locus_tag="PK610_02945"
     CDS             complement(5165..6529)
                     /locus_tag="PK610_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HRJ34911.1"
                     /translation="MSKAIKIKRGLNIRLKGEAEKVLVSLPVSGTYAVKPTDFHGLTP
                     KVLVKEGDAVKAGSVLFIDKYNDRIRFVSPVSGKVSAVVRGEKRKVLEILVQADAETS
                     YETYSSADPKSLSREALIESLLKSGLWPLLRQRPFSGVANPADMPKSIFVSCFDTAPL
                     APDYDFIVHGQGEDFQLGVDALSKLTNGKVHLNVSGSLGQSKVFSNSRGVQINTISGP
                     HPAGNVGVQIHHIDPINKGDIIWYINPQDLIIFGRYFRTGKTDFSKVVALTGGHVKNP
                     KYYRVVTGATMAQITEGNINESEEVRYISGNVLTGSNAGKNGYLGYYDNQVTVIEEGN
                     KYKFFLTQGWLSPGFGKFSASRTFPTWLMPGKKYNLDTNINGEERAFVMTGQYEKVFP
                     FDIYPVQLIKAILANDLEGMENMGIYEVDPEDFALCEFVCTSKINSQEIVRGGLDLVK
                     KECF"
     gene            6698..7978
                     /locus_tag="PK610_02950"
     CDS             6698..7978
                     /locus_tag="PK610_02950"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HRJ34912.1"
                     /translation="MGSVENTGVNGGKDNSDNYTGIPRKEMKHEDQVYYQSIHTPLCY
                     RTGFDLQPPVIALNTQETVTISFDDFETNIKDLYYGLVHCDANWKPDNLMEQEFLEGF
                     FTYNISSYKYGFNTYQPYIHYNFQLPNSYTKITRSGNYIVKVFANNDPTQLILTRRFV
                     VYENQVDIRWQYKRPQDPEHRTTKQEIDFSILYGSLNVPDPINDLKVVLLQNNRWDNA
                     IRNLKPMFVRDRELVYDLDEPNIFNGINEYRFFDLKSLNYNTPNVARILKDTIPYHVF
                     LLNDERRPYKKYITSFDINGKFLIKSDLANDPHTESDYVWVHFYLPYDTPIDSGDIYL
                     FGQFSDWQAKEEFKLRYNYRTKRYEHALYLKQGFYNYHYAIWNDKSKRIDETLVEGNH
                     FDTENDYTILVYFYDIRANCDRLIGVRNLNSVKN"
     gene            8034..8420
                     /gene="apaG"
                     /locus_tag="PK610_02955"
     CDS             8034..8420
                     /gene="apaG"
                     /locus_tag="PK610_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HRJ34913.1"
                     /translation="MITQVSQGVSVSVFTSYEEELSNPLNRHFIFTYRIRIENQNNFT
                     IQLLRRHWEIQDSLSFPREVEGEGVIGEQPLLEPGHWYEYESACNLTSNVGSMKGTYL
                     FQRVDNGNLFQANIPLFMLVTPDKLN"
     gene            complement(8770..9198)
                     /locus_tag="PK610_02960"
     CDS             complement(8770..9198)
                     /locus_tag="PK610_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HRJ34914.1"
                     /translation="MQYNSRLYLHEVIDHVRAFHDAFLIDNHHSPTVLVKEQEYMLRH
                     RLMQEENDEYLDACRKGDIIEIADALGDQLYILCGTILRHGLQHKIEEVFLEIQRSNM
                     SKLDANGQPIFREDGKVMKSELYTRPDIKKIIHENSEILS"
     gene            9332..>9809
                     /locus_tag="PK610_02965"
     CDS             9332..>9809
                     /locus_tag="PK610_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ34915.1"
                     /translation="MLETPSVGNQASSNQGFDIRELNERIQRESAFVDLLIHEMDKVI
                     VGQKYMVERLMIGLLSNGHILLEGVPGLAKTLAIKTLSETIDAKFSRIQFTPDLLPAD
                     LVGTMIYNQKKEEFTVKMGPIFSNFVLADEINRAPAKVQSALLEAMQERQITIGENT"
BASE COUNT         2926 a         2207 c         1957 g         2719 t
ORIGIN      
        1 tcttaatcaa agaatctgag aaagaccttc caattggagg gtcttttttt ttgaagcaat
       61 ataatcacac acaacttata ctatcttggt tcaacattca attgaaaaaa atcatcctta
      121 attcaaatta tcaatggcaa aaataattgg cctcggcggc atctttttca aatcccctga
      181 tccggctgca ttgcgtaaat ggtactgtga agtactagga ataaaagcag aagaatgggg
      241 aaccgttttt ccgtttaccg aaaatgaatc cggatacaat gtttggagcg ttatgaaatc
      301 cgataccgaa tatttctcac cttccacttc atcctttatg atcaatttcc gtgttgatga
      361 tctggatgta ttcattgctg aattgatcaa aaagaacatt agcctttgtg gtgatcctca
      421 aatgggcgaa tacggaaaat ttgcatggat acttgatccc gatggaaaca agcttgaact
      481 ctggcagccg ccgcagtcct gatacggtaa ataaaaaagc cccgcacatg cggggctttt
      541 tttatgctga ataaattaac ctccaaagtc gtcgaagcga acattctctt tgggtacacc
      601 ccaatcgtcg cacatttttg ttacggcgtt gttcatcatt ggtggaccac agaaatagaa
      661 ttcaatatct tctggagaat cgtgctttga taaatattga tcgatcaccg cctggtgaac
      721 aaaaccaacg aaaccatctc cggcagtatc attgatgtct tttttcacta cccaattatc
      781 ttcaggaagg ggttcagaca aaacaagata aaacttgaaa ttagggaagt ctttctccag
      841 atcgcggaaa tagtggatgt aaaataactc ctctttagaa cgaccaccgt accaatatga
      901 aactttacgt cctgttttaa tcgttttaaa cagctcgtaa agatgcgaac gcataggagc
      961 catacctgca cctccaccaa cgtacaacat ttcagcatcc gattcattaa tgaagaattc
     1021 accatatgga ccagaaataa ctgctttgtc accgggtttg aggttgaaga tgtaagaaga
     1081 cgcaataccg ggattaacat tcatccaagt attttttgca cgatcccatg gtggtgtagc
     1141 tacacgaacg ttaagcatga tcttacgtcc ttctgcagga taagaagcca tcgaataagc
     1201 acgaaccact tcttcgttat tcttcattac cagatcccac attttgaatt tatcccaatg
     1261 cttgcggaat ttatcaggtt cacccgggtg atcctgcgga tgggctgcaa tatccatttc
     1321 ggaatacttc actgtgcatg caggaatttt gatctggata tatccaccgg ctttgtagtc
     1381 catgtcctct ggaatctcaa cgataaactc cttaatgaaa gtagctacat tatagttgga
     1441 aaccactgta gcttcccatt ccttgattcc aagaatttct tcgtgaatat ggattttcat
     1501 atcctgtttc actttaacct gacaaccaag tctccagtga tctttcactt cttttcttga
     1561 aaagtgaggt acttctgttg gaagaatctc tcctccacct tcaacaacct ggcacttaca
     1621 ctgtacgcag gttccacctc caccgcaggc agaaggaaga aaaatcttct cattccccaa
     1681 cgtggaaagc aaagaacttc cggcgtttac ttcaagtact ttatcatcat tgataatgat
     1741 tttcacttta ccggaggcca ccagtttcga cttagcgtat agcagaagcg caaccagcaa
     1801 taaggttaca cctaagaata cgagaagggc cattacaaga gtctgtgtca tgacaatcgt
     1861 ttttgcaatg attaaaattt aattcccatg aagctcatga aggctatccc cataagtccg
     1921 gttagaatga aagtaatacc caaaccacga agcggtgcag gaacatgaga ataacggatt
     1981 ttctcacgaa tagcagcaag caaaacaatg gcgatgaacc atcctattcc actaccaagc
     2041 gcaaatgaag ttacatgagc aatacttgga tactgacgtt cctgcataaa gagcgcgcca
     2101 ccaagaattg aacagttaac agcaatcagc ggaaggaaaa tccccaacgc tccgtaaagc
     2161 gcaggaagaa acttttctac cgacatctct accagctgaa caaatgctgc aaccacagca
     2221 atgaacacaa taaaggaaag gaagctcaga tcgatcgatt tgtactcctc acccatccat
     2281 gccaatgcac ctggtttcaa aaaataattt tcgataaggt agttcaaggg aacagtcatc
     2341 gttaatacaa agatgacagc cataccaaga ccaacagctg ttttaacagt ttttgatacc
     2401 gcaagatagg agcacattcc aaggaagaat gcgaagatca tattgtcgat aaagatcgac
     2461 ttgatgaaaa tatttagtaa ttccatttct tctgtagttt agaaatcaat gtgcttcgat
     2521 gagtttggta ttccttgcgc gctgggccca gatgtacaat ccaacaagta tcaaggccat
     2581 tggtggcatg atcatgaagt tgttcagtac ataccatcca tcaggattat ccattgaata
     2641 gatccaattt gcaggtaata cctgagcatt ccacaatttg cctgagccaa ataactcacg
     2701 aacaattcca aggaaaataa ggatgatacc gtatccaata ccattaccta atccgtcgag
     2761 gatagatgga ccgggtttgt tacccaaagc aaatgcttcg atgcgtccca taatgataca
     2821 gttggtgatg atcagtccga caaacaccga aagactctta cttacatcgt aaacgtatgc
     2881 tttaagaacc tggtcgacga taataaccaa ccatgcaaca acaacgagct gtacgatgat
     2941 acgaatacgg ttaggaacga tattccgcat cagcgacata aagaagttcc ccataatagt
     3001 aacagctgta acggaggccg ccataactac tgaattatcc agttttacgg taacagccaa
     3061 tgccgaacaa atacccaata cctgtacagt aataggattc gaatcattaa gcggatcggt
     3121 gatcagcttg cggttctttt tcgaaaagag tccttctgaa ggagactttg cgggagcggt
     3181 agcttcactc atatactcag tttaatgcgg ttgatttaga catggtctta aaatatttgc
     3241 tgtaaatact catgcaacgc atcagcattt catctacacc aacagatgtg aatgttccgc
     3301 cggagattcc atctacagca tgatcggatt ttggtccgcc cggttttaca acacgaatag
     3361 aaacaaactc tccgttgtca tcatgaattt tctttccagt gaactgtgtt tcgaaaaacg
     3421 gagtggcaat ctcagcaccc aatccaggcg tttcgctctt gtgatcgaat gatgcaccgg
     3481 cgatggtatt caggtcttcg ccaactgcaa catatcccca aacaccagcc cagagacctt
     3541 gtccgcccac aggcattaca aaaaatcggc taccgtcttt ctcacaaata aaaagtgggt
     3601 atctgcgatc tgtagcaggc ttatccttga actcagaaag cgcattgatc gttaaagctt
     3661 ctgcaattac atccgtcttt acttcaacag cctgactatt tgcatcaaga ataactgcgt
     3721 ctttaacgta ttgctcaaac tttgttcctg catcctgcag cgtagcttca acctgtatgg
     3781 ccgcaagaat gttttgtttc ttatcgttca tcacgttttt ctcctgaacc ggcttgaggg
     3841 tttgggaaac gattaccagt gatgttccta ctaccacaca catcgcaatg gcgaagatga
     3901 tggtatatgc gttactgtct ttattgatgg ccataattaa gcagttttaa gtttggcacg
     3961 gttaaggcga cgcttgatgt tagcctgaac aacgtaatga tcgattgtgg gtgcaaatac
     4021 attgcttaac agaattgcca tcatcacacc ttcggggaat gcagggttga caaccctaac
     4081 aagaatggaa agcattccgg ccaatacacc gtaaataatt tttccggtag gcgtttgtgc
     4141 tgctgttaca ggatcggtaa ccataaacac cataccgaac ataaatccac ccatcatcaa
     4201 ctgatgcatc ggatcaactt ccatgtatgg atttgcgccc catgcattga aaatggtttg
     4261 cattaaaaat ccacctgcaa acatagatgc cataatacgg aaagaaccaa taccggtaag
     4321 aatcaaaatt cccgctccaa tcaaaattgc aagtccggaa gtttctccca ctgaaccagg
     4381 ctcaatacca atgaaagttg tccataaatc ggccactttt tcattcatta caacatcttt
     4441 cgtttccggg ttggtgacca agcctgcaag gtgaccaaga ttagtagcgc cggtagtacc
     4501 gtctacaaac tgtgttttgt caccactgat ccaaacttca cctgccataa acgttggata
     4561 ggcaaagaag agaaatgcac gtgcagttaa tgcaacgttt acaatattca ttcccgttcc
     4621 gccaaaaact tctttcgcaa ttaccaccga gaaagcagta gcaacagcta ccatccaaag
     4681 cggtacatca gcaggcatca caagaggaat aagcattcct gaaacaagaa atccttcctg
     4741 aatagcgtgt cctttggcga tacagaatgc gaactcaatt cccaatccaa caccgtaaga
     4801 aaccactaca agcggcagca ccttaatcag tccgtacaca aactcctgca tcattgtagc
     4861 aggaattccc aaggctgaat aatgccagta accaacattc cacatcccaa aaaccaaaca
     4921 agggatcaag gcgatgatca ccgtcatcat tgtacgcttc aggtcgatac tgtcgcggat
     4981 gtgcgcaccg gaatgggtgg catggttggg ggtaaatgcg aaagtggcaa atccatcgta
     5041 caaagggtgc agcttttcaa acttgccgcc cttcttgaac gaaggctcca tctttttcat
     5101 catgttttcc agaaacttca taggtgttct ctgtatggta taaacaattc tagttgactg
     5161 tagtttagaa gcattccttt ttcacaagat caagtccgcc gcggacaatt tcctgagagt
     5221 tgattttgga agtacaaacg aattcgcaaa gcgcaaaatc ttcaggatct acttcgtaaa
     5281 tacccatgtt ctccattccc tcaagatcat tcgcgagaat ggctttgatg agctgaacag
     5341 gataaatatc gaatggaaaa accttttcgt actgaccggt cattacaaat gcacgctctt
     5401 ctccattaat gttggtgtcg agattgtatt tcttaccggg cataagccag gttgggaaag
     5461 tacgtgatgc tgaaaatttg ccgaatccgg ggctaagcca gccttgagta aggaagaatt
     5521 tgtatttgtt tccttcttct attacagtaa cctgattatc ataatacccc aggtatccgt
     5581 tcttaccggc atttgaacct gtaagcacgt ttccggagat ataacggact tcttcgcttt
     5641 cgttaatgtt accttcagta atctgggcca tagttgcgcc ggttactaca cgataatatt
     5701 taggattttt cacatgtccg ccagtaagag caacgacttt actaaaatct gttttccctg
     5761 tacggaaata gcgtccgaaa atgataagat cctgtgggtt gatgtaccag ataatatctc
     5821 ctttatttat aggatcaata tggtggattt gcacgcctac atttcctgca ggatgcggtc
     5881 cgctgatggt gttgatctgt acaccgcggg aattggaaaa tacttttgac tgtccgagac
     5941 tgccactaac attaagatga acttttccat tggtaagttt actcaaagca tccacaccaa
     6001 gctggaaatc ctcaccctgt ccgtgtacga taaaatcgta gtctggcgca agaggagctg
     6061 tatcgaaaca gctcacaaat atcgatttgg gcatatcagc cgggttggca actccggaga
     6121 aaggacgttg acggagcaat ggccataagc cagactttaa cagactttct attagagctt
     6181 ctcgcgaaag tgatttcgga tcggcagaag aatacgtttc gtagctggtt tcagcatccg
     6241 cttgcacaag aatctcgaga actttacgtt tttctccacg aaccacagcg cttaccttac
     6301 cgctaacagg tgaaacaaaa cgaatgcgat cgttatactt gtcgataaac aatactgaac
     6361 cggcctttac agcatcacct tccttaacaa gaacttttgg tgtaagtccg tggaaatcgg
     6421 tgggtttaac agcatatgtt ccactaaccg ggagcgagac gagaactttc tctgcctcac
     6481 ccttgagccg gatgttcaag ccgcgcttga tctttatggc ttttgacatg gaaaaggatg
     6541 tttggtacgg gggcaaaatt ataaatttgt tgaacccaac aaaccttttt gtcgttttat
     6601 aaactttcat tgaatcaacc gcctgatgtc aacatcttac aatagactct accgtgcctc
     6661 aggcatactc ttttccttaa tttttgtcgc ctgtaatatg ggttccgtcg aaaacacagg
     6721 agttaacggg ggaaaagaca attcggacaa ctacaccggg attccccgaa aggaaatgaa
     6781 acatgaggat caggtttact atcaaagcat ccatactccc ttgtgttacc gtacaggatt
     6841 cgaccttcaa cctcctgtaa ttgcgcttaa tacacaggaa acggtaacta tttcatttga
     6901 tgatttcgaa accaacatca aggacctcta ttacggattg gtccattgtg atgcaaattg
     6961 gaaacccgat aatctgatgg aacaggaatt cctggaagga ttctttacct ataatatcag
     7021 ttcctataaa tacggtttta acacctatca accctatatc cattataatt tccaactccc
     7081 caattcttac acaaagatca cacgatcagg aaattacatc gtaaaagtgt ttgccaataa
     7141 cgatccaact caactcattt taacccggcg atttgtggtt tatgaaaatc aggtagatat
     7201 ccggtggcag tataaacgtc cgcaagatcc agagcaccgt acgaccaaac aggaaatcga
     7261 tttcagcatt ctttacggtt cactcaatgt acccgaccct attaatgacc ttaaagtagt
     7321 gttgttacaa aacaaccgtt gggataatgc tatacggaat ctgaagccca tgtttgtccg
     7381 cgatcgtgaa ttggtatatg atcttgatga accgaatatt ttcaacggta ttaatgagta
     7441 ccgctttttc gatttaaaga gtttgaacta caatacacca aacgttgccc ggattttgaa
     7501 ggacaccatc ccctatcatg ttttcctctt aaatgatgaa aggagaccct ataaaaaata
     7561 catcaccagt tttgacatca acggaaagtt cctgatcaaa agcgatcttg ccaatgatcc
     7621 gcataccgaa tcggattatg tttgggtgca cttttatctt ccctacgata cgccgatcga
     7681 ctccggtgat atttatttgt ttggtcaatt ctccgattgg caggcaaagg aagaattcaa
     7741 attaaggtac aattacagaa ccaaacgtta cgaacacgcc ttgtatctga aacaaggatt
     7801 ttacaattac cattatgcca tttggaatga taaatccaaa cgcattgatg aaacccttgt
     7861 ggaaggaaac cattttgaca ctgaaaacga ttataccatt ctggtctatt tttatgatat
     7921 cagggccaat tgcgatcgac ttattggtgt aagaaacctc aattccgtta agaattgacc
     7981 attccctaaa caggatttgg aaaaaacgat ggaagtctta tttttggttc gctatgataa
     8041 cccaagtgtc acaaggtgta agtgtaagtg tctttacaag ctatgaagag gaattatcca
     8101 atcctctgaa ccgccacttc atttttacct atcgcatacg tatcgagaat cagaataatt
     8161 ttaccataca attattgcgt cgccattggg aaattcagga ttcgctttct tttccgcgag
     8221 aagttgaagg cgaaggcgtt atcggagaac agccactttt ggaacccggt cactggtacg
     8281 aatacgagtc tgcctgcaac cttaccagta acgtaggaag tatgaaagga acctaccttt
     8341 ttcaacgcgt cgacaatggc aatttatttc aggcaaatat tccattgttc atgcttgtga
     8401 cgccggacaa attaaactag gcttcgtttc tcagcacttt tttcgggacc ctgaattagg
     8461 caattcttgt cattgatttt ttcccaactc aagtcgtaaa tactgcaacg taatcgtcca
     8521 tcgggcaaat gcgataaatg cgtaaggaat ccaaaccgaa acccccgaat ttccaattcg
     8581 tttagcggta attctctttc agattggttt ccgatacacc gttcaaggat caaataattg
     8641 gtacggatga tcctgtttac attttttgcc aggttttggg tgatcctgtg gttgcggttg
     8701 tggtattgaa ttcggcaggc gtcagagcaa tatttcttat cagctctacc gctaacggca
     8761 gatccgcaat caagacagaa tctcggagtt ttcatggatg atcttcttaa tgtcaggtct
     8821 ggtgtacaat tcacttttca tcacctttcc atcttcccgg aagatcggtt gtccgtttgc
     8881 atcgagtttc gacatgttgc tgcgctggat ctccagaaat acttcctcga tcttgtgctg
     8941 caaaccatgg cgaagaatgg tcccgcaaag tatgtacaac tgatcgccca gtgcatccgc
     9001 tatttcaatg atgtctcctt ttctgcaggc atcgaggtac tcatcattct cctcctgcat
     9061 cagacggtgc cggagcatgt actcctgctc tttcaccaaa acagtgggag aatggtggtt
     9121 atcgattaaa aatgcgtcat gaaaagcacg gacatggtcg atgacttcgt gcaaataaag
     9181 tcgggaattg tattgcatgc aaaccagatt tgggttccaa tttaagtttc ggcgggagat
     9241 aaacagtata aaaatgctta acaattttta acagcggtta ataaccctgt ggcgtattaa
     9301 aggagaaatt tgctttaata aatccactat catgctagaa actccatccg taggtaatca
     9361 ggcttcttca aaccagggct ttgatattcg agaattgaac gaacgtatac aaagggaaag
     9421 tgcctttgtt gatttgctga tccatgaaat ggataaggtc attgtcggac aaaaatacat
     9481 ggtagaacgc ttgatgatcg ggctattgtc gaacggacat atccttctcg aaggagttcc
     9541 gggtctggca aagacccttg ccatcaagac cttatcggaa acgattgatg cgaagttcag
     9601 caggattcag tttacgccgg atttgttgcc tgccgacctt gttggaacca tgatctacaa
     9661 ccagaagaaa gaagagttta ctgtgaaaat gggtcccatc ttttcgaatt ttgtgttggc
     9721 cgatgagatt aaccgtgcac cggcaaaagt gcaaagtgcc ctactggaag ctatgcagga
     9781 aaggcagatc accattgggg aaaatacct
//
LOCUS       DAOMYG010000055         9760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2320_length_9760_cov_11.746522, whole genome shotgun sequence.
ACCESSION   DAOMYG010000055 DAOMYG010000000
VERSION     DAOMYG010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 9760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9760
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2320_length_9760_cov_11.746522"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PK610_02970"
     CDS             <1..430
                     /locus_tag="PK610_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRJ34916.1"
                     /translation="HTQFIQSLISEGKLKLKEGGEFKGKRITFHDPCYLGRANDVYEA
                     PRELIQKLDVELVEMKRCKQKGFCCGAGGAQMFKEAEPGNKEVNVERTEEALALQPQV
                     IATGCPFCNTMMTDGVKNFNKENDVKVLDVAELIANAADL"
     gene            434..916
                     /locus_tag="PK610_02975"
     CDS             434..916
                     /locus_tag="PK610_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="HRJ34917.1"
                     /translation="MAEWTSLPEHSRVWIYQADRLLSATEASWVKEQAIAFVSNWSSH
                     GAKLEASVELFDQLFLVVFVNEDQAAASGCSIDKSVAFVKAIEKELGLSFMDRMKTAI
                     ATNDGPELVALNDFPRLKSEGKISDTTLVFDNLVNTKAEFERGWKKRIADSWHMRFLN
                     "
     gene            1013..7978
                     /locus_tag="PK610_02980"
     CDS             1013..7978
                     /locus_tag="PK610_02980"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ34918.1"
                     /translation="MLRCLAIAIFFLFSIPESFAQPLNANAGPNKNICSGASTTLGAT
                     PAATGGTGSYTYSWSPATGLSCTNCPNPVCTATTSTTYTLTVDDGNNTDNDNVNVNVT
                     QGATASFTFSPNNSCSSIPVVFANTSTPLAGSSFQWNFGDPASGSANTSTQMNPTHTF
                     VAPGNGTQTFTVSLTVTSSTGCTHTTTQNVTVNQSPAPELIDPFTSFRNCDGSIFNME
                     VFDNTNPTTNNSYVINWGDGTPDFVAGPGPFPSGSVTHIYSTMGIYTLTYTVTGTNGC
                     ANTFTQFVSNITNPAIGVSNPGNTNGCGPITLCFPLSSFAGNHSSTTYRVNYGDGSPE
                     DIFTHPPPATVCHTYTQSSCSSAGGAYTFSITARNSCDSSTATVTPIRVYTAPQANFT
                     ESPNPACVNSAVTFINSSVAGFNASCSQSTVHVWQWGDGTPNTTVFTNAPQTHTYTAP
                     GNYTVVLSTSNGCGTTTMTRTVCIEAPPVPNFTLSPTSGCVPLISNITNLSDTSLSCD
                     VNWNWVVTFNGSPCVPASGAFSFTNGTNANSWQPQITFNSQGTYTVTLQLINSCGTFP
                     ISQTVTVHKVPEITFPALPNICAGQSITPTATFLDCTAPITTYNWIFPGGVPATSNLQ
                     IPGSVSFAAAGNPTITVSATNICGTTNATQSVTITAAPPAPVAGSNSPLCEGATLDLT
                     ASNIANANYQWSGPNGFTATTQNPSITNVTVANAGSYSVFATVNGCAGPSASTNVVIN
                     PAPVVTIVPVNPSICAGQSVLLTGSGASSYVWNDGTGNVGTNATLNVSPAATTTYTLT
                     GTTAGCSAQVTTTVTVNPVPIVNAGPDLTLCNQPIGEQLNGTPAGGTWSGTGITPGGL
                     FTPSTVGVFVMTYSFTDGNGCTSTDNASITVNDPVPVVMGSNQSVCLNAAPINVTANP
                     AGGVWLGTGVSAAGIFTPSAIGTFTLTYTLGTGTCATSGTLDVTVNPLPVINPGAPIN
                     VCFDAAVQNLNATPPGGTWSGSGVSGLTFDPALAGVGVHNLTYSFTDGNGCSNTGNLS
                     TTVNALPIVNAGADQIVCDQPIPVVLSGTPAGGTWTGPNVTAGGSFTPNGTGVFALTY
                     SFTDGNGCSSNDQMNVTVVAPTPADAGLPQSVCHNAAAITLNGIPAGGTWSGTNITAG
                     GIFTPSTVGVFTLTYSFGTGTCATTDDVQVTVNALPIVNAGVDFQLCVDAAPLNLNGI
                     PAGGTWSGTGITNPSGVFSASVSGVGAHVLTYSFTDGNGCTNTDQLDATINALPVVNA
                     GADTTVCDLPIPVTLTGTPVGGTWSGANITAGGVFTPNGTGAFTVTYTFTLGTGCTAS
                     DDRVVNVIAPQQANAGPDQEICHNAAAITINGLPAGGTWSGSFVTAGGLFTPSTPGTY
                     NLTYTFGVGNCQTTDVMQIIVHPLPVVNAGVDQNICIDAAVTILTGTPVGGSWSGIGI
                     TNAALGTFDPFVAGVGAHNITYTFTHPVTGCLNSDVMVMNVQPLPVMGFSNNPIACVG
                     IAEPFTNTTTGAISYAWNFGDGNTSTQTNPQNAYAAVGTYTISLTATSLFGCVNSITG
                     SIDVFEPPVANFTLAPDSACGPLVVNFNNISSGQSISYNWDFGNGTTSALAAPGAVTY
                     PAGYLADTTYTIVLSVSNFCATVTHTEQVIVMPEPKAIFGTNVNVGCSPFTPEIANNS
                     VGLPDTYFWNFGDGTTGNQADALFGHTFFTGLEDTTFTVMLVVTNECGVDTAYHTITV
                     LPNTVNAFFNSSDTSGCVPLAVNFTQFSTGATFSSWDFGDGNVSTAPNPSHTFTQAGT
                     YLVQLFINDGCSFDTATISITVHPQPQIDFTTTPDSVCASQPFQFNNLSSNLASVNWN
                     FGDGNTSVLTNPIHTYGLSGTYMVTLSGVSTVFGCSTSVTRPVIVRTTPVASFVPDPL
                     FGCEPLPVSFQNTSTQAQFFSWNFGDGNSSVQVNPSHTYATSGTYTITLIATNLNGCS
                     DTAVQSVLVNPNPIAAFSTSSPSSCYSPVTVNITNLSSGAVGYSWNLGNGQTSALTNP
                     SVTYTQPGSYTITLIAETPFGCLDTATYVYVVYPTPVANFSIPSDTSCELYPVPFTNL
                     SQNATSYQWSFGDGSTSAQQNPVHNYADSGLYSVTLMVYGAGGCGDTITLNNTLTIFP
                     SPTAGFTWINVQNVDPLSGTVEFTNTSTGAIGYEWHFGNGQTSIEVNPVHRYNRYGNW
                     SAYLVATNEYGCMDTAYQNVVVDFFKGLFAPNAMYPGHPDFGVAHFIPTGVGLKSFRV
                     SIYDDWGNLIWECSDIVDTRPACAWDGTFNGAPVQQDAYVWKIEAFFLDESLWVGQLY
                     PNGLYRAAGTVTVIR"
     gene            7975..9021
                     /locus_tag="PK610_02985"
     CDS             7975..9021
                     /locus_tag="PK610_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRJ34919.1"
                     /translation="MMKFFKLFFALLLIHADVFGQDPNFSQFFHNPIYYNPANVALDN
                     GLRVRGNVRNQWGPIPGRFNTGCFSMDAAGINKTGLGIIALSNNEGEGRLRTMQAGLM
                     YSYRPVETKNFRLQAGFGASFVQKRIDWSRFVFSDQLDEVFGNVSATSFIPPGTDRVV
                     YPDFNAGVSARFNSGKVEEKGGKMTTTIGAGFHHLTRPKDAFVSDGNRLPLKMVIHGH
                     SNILSYNGVIYSPGFIYERQSNFTTFQVGINMYKNPVYAGFWFRNRNLNMQGKNYDSF
                     VFVLGLNSFMAHQRRAKFCYSYDFTISRLKTSSFGTHEFSVVWELNDIVTFSRIEKNK
                     RKRQKTRFLECVDF"
     gene            complement(9023..>9760)
                     /locus_tag="PK610_02990"
     CDS             complement(9023..>9760)
                     /locus_tag="PK610_02990"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HRJ34920.1"
                     /translation="MDSLISTVDSIAFTTEKIELKEGVCDKPDQSCITVNFEYPVFAE
                     THSAGTPLNTWIRDQITTPDEKGNVRSQKEFAEYLITLFKNDKNAIGDDMMPWSVENI
                     AGINFENKGLISLYVSGSSYTGGAHPNSFTSYGSFYKSNGKKITISEIVKDSVAFVKI
                     CEKHFRSFNGISPETDLTEQGFEFGDEGFIINTNFYISDRSITFLYNNYEIGPYIMGS
                     WAFSVPFADIQEQLTSDFKALIEIPIN"
BASE COUNT         2605 a         2157 c         2205 g         2793 t
ORIGIN      
        1 ccatacgcaa ttcattcagt cgctgatttc cgaaggaaaa cttaagctga aagaaggagg
       61 cgaattcaaa ggcaaacgca ttacgtttca tgatccctgt tacctgggca gagcaaacga
      121 tgtttatgaa gctccgcgcg aactgatcca gaagcttgat gttgaattgg tggaaatgaa
      181 gcgctgcaaa caaaaaggtt tttgctgcgg tgccggtggt gcacagatgt tcaaagaagc
      241 ggaaccagga aacaaggaag tgaatgtgga gcgcactgaa gaagcccttg cattgcaacc
      301 tcaggtgatt gcaacgggtt gtcccttttg caataccatg atgacagatg gcgtaaagaa
      361 tttcaataag gagaatgatg tgaaggtgtt ggatgtagct gagcttattg ccaacgctgc
      421 agatctttaa ggtatggctg aatggacctc actccctgaa cattcgcggg tatggattta
      481 ccaggccgat cgtttgcttt cagcaacaga agcatcatgg gtgaaagaac aggctattgc
      541 atttgtaagc aactggagct cgcacggggc taaactggag gcttcggttg agttgtttga
      601 tcaattgttt cttgttgtat ttgtgaacga agatcaggca gctgcatcag gctgttccat
      661 cgataaaagt gttgcttttg ttaaggcaat cgaaaaagag cttggtcttt cgtttatgga
      721 tcgtatgaaa acggcaatcg ctacaaatga tggtcctgaa ctcgttgcat taaatgattt
      781 tccacgacta aaatcggagg gaaaaattag tgataccaca cttgtgtttg ataatctggt
      841 gaacacaaaa gctgaatttg aacgcggctg gaaaaaacga attgccgatt cctggcatat
      901 gcgttttctg aattaaatct gcttagaatc atgatattgg atggcaaatt agggtgaata
      961 ccgtagtttg gatcatagtc aattgtacta tgtttgccat ggaacctcta ttatgttgcg
     1021 ctgccttgct atagccattt tctttctgtt ttcgattccg gaatccttcg ctcaaccctt
     1081 aaacgcgaat gccggtccga ataaaaatat ttgctccggt gcttcaacca cacttggtgc
     1141 aactcctgct gctacaggtg gaacgggttc gtatacctat tcttggtcgc ctgcaacagg
     1201 attgtcctgt acgaactgtc caaatcctgt ttgtactgca accaccagca ccacctatac
     1261 cttaactgtt gatgacggaa acaacaccga caatgacaac gtaaacgtaa atgtaacgca
     1321 gggagcaact gcatctttta ccttttcccc gaataattcc tgttcaagta ttccggttgt
     1381 atttgcaaac acatccactc ctttggcggg ttcaagcttc caatggaatt ttggcgatcc
     1441 tgcaagtggt tcggcgaata cgtcaacgca gatgaatccc acccatacat tcgttgcacc
     1501 gggtaacggg acacaaacgt ttaccgtttc tctcaccgtt acgagttcaa ccggttgtac
     1561 acacaccacc acacaaaacg tcaccgtcaa tcaatctcct gctcctgaac tgatcgatcc
     1621 ctttacttcg tttcgcaatt gcgatggttc cattttcaat atggaggtgt tcgataatac
     1681 gaatccaact accaataatt cctatgtgat caactggggt gatggtactc cggattttgt
     1741 tgccggtcca ggaccatttc cttcaggctc ggtaacacat atatattcta ctatgggtat
     1801 ttatacgcta acctacaccg taaccggtac caatggttgt gcgaatacat ttacccaatt
     1861 tgtttccaat atcaccaatc cggccatcgg ggtttccaat ccaggtaata cgaatggttg
     1921 cggacctatt accttgtgtt ttccattatc aagtttcgcc ggtaatcact cttcaactac
     1981 ttaccgtgta aattatggtg acggatcgcc ggaagatatt tttacgcatc caccaccggc
     2041 aacagtttgt catacctata cgcaatcttc ctgttcttct gctggtggtg cgtatacttt
     2101 ttccattacg gcacgaaaca gttgcgatag ttcaacggca acggtaacgc ctattcgtgt
     2161 gtataccgct cctcaggcaa attttacaga aagtccgaat cctgcctgcg tcaattcagc
     2221 ggtgacattt atcaattctt cagttgcagg gtttaatgca tcctgtagcc agagtacagt
     2281 acatgtgtgg caatggggcg atggaacacc caatacaaca gtttttacca acgcacctca
     2341 aacgcatacc tatactgcac caggaaacta caccgtagtg ttgagcacta gtaacggttg
     2401 cggaacaaca accatgacgc gtactgtttg cattgaagca cctcctgttc ccaattttac
     2461 cttgtcgcct acttcgggat gtgtgccttt gataagcaac attaccaatt tgtcggacac
     2521 ctcactttct tgcgatgtga actggaactg ggtagtaaca tttaatggtt caccttgtgt
     2581 gcctgcaagt ggagcattca gttttaccaa tggaaccaac gccaacagct ggcaaccgca
     2641 gatcacattt aattcacagg gtacgtatac tgtaacactg caactcatca attcgtgcgg
     2701 gacatttccg attagccaga cggttacagt acacaaagtt cctgaaatta catttcctgc
     2761 tttgcccaat atctgcgccg gacaaagcat cacaccaacg gcaacatttc tcgattgcac
     2821 agcaccgatt accacctaca actggatatt ccccggagga gttcctgcaa catccaattt
     2881 gcagattccg ggatcggtta gttttgcagc agcgggaaat ccgaccatta ccgtttctgc
     2941 aacgaacatc tgcggaacaa caaatgccac tcagtcagta acgataacag cagcacctcc
     3001 tgctccggtt gcaggtagca attctccctt gtgtgaagga gcaacacttg atctcactgc
     3061 ttcgaacatt gccaatgcca attaccaatg gagtggtcct aacggattta ccgcgacaac
     3121 acaaaatcca tcgatcacca atgttacagt agccaatgcc ggatcgtatt cggtatttgc
     3181 aacggtaaac ggttgtgcag gaccttcagc atccaccaat gttgtgatca acccagcacc
     3241 ggtagttact atagttcccg taaatccgtc tatctgtgca ggacaatcgg ttctgcttac
     3301 aggcagtggt gcatcaagct atgtttggaa cgacggaaca ggaaatgtag gaacgaatgc
     3361 aacattgaac gtttcacctg cagcaacaac aacgtataca ctcacaggaa caacagcagg
     3421 atgcagtgca caggtaacga caaccgtaac ggtgaatcct gtgccgattg taaatgcagg
     3481 tcctgattta acgctttgca atcaacccat cggagaacaa ctgaacggaa caccggcggg
     3541 cggaacatgg agcggaacag gaattactcc cggcggattg tttacgccat cgacggtagg
     3601 agtttttgtg atgacctatt cctttaccga tggaaacgga tgtacatcca ccgataatgc
     3661 cagtattacc gtcaatgatc ctgttcctgt agtgatggga tcgaatcagt cggtgtgttt
     3721 gaatgcggca cccatcaatg taacagcaaa tcctgcagga ggagtgtggt tgggtaccgg
     3781 tgtaagtgct gcaggaatat ttactccttc agcaattggt acattcactt taacctatac
     3841 gttgggaacc ggaacttgtg ctaccagcgg aacattggat gtcacggtga atccccttcc
     3901 tgtgatcaat cccggagcac caatcaatgt ttgttttgat gcagctgttc aaaatctgaa
     3961 tgcaacaccg ccaggaggga cgtggtcggg tagtggagtt agcggtctca ccttcgatcc
     4021 tgcgcttgcg ggtgtgggag ttcataacct cacctattca tttacggatg gaaacggttg
     4081 ttcgaatacc ggaaatttat cgacaactgt aaatgcactg cccattgtaa atgcaggagc
     4141 agatcagatt gtttgcgatc aacccattcc tgttgtacta agcggaacac ccgcaggagg
     4201 aacatggacg ggaccaaatg taactgcagg aggatctttc acacctaatg gaacaggtgt
     4261 atttgcactg acttattcgt ttaccgatgg taatggatgc agcagcaatg atcagatgaa
     4321 tgtaactgtt gttgcaccca caccggccga tgcaggattg ccacaatcgg tttgtcacaa
     4381 tgcagcagcg ataacgctta acggaattcc cgccggagga acatggagcg gaacgaatat
     4441 cacagccgga ggaattttta ctccttcaac ggtcggtgta tttactctta cctattcatt
     4501 cggaacagga acctgtgcaa ctaccgatga tgtgcaggtg acagtaaatg cattaccaat
     4561 tgtaaatgca ggagtcgatt ttcaactgtg tgttgacgct gctcctctaa atttaaacgg
     4621 aattcccgca ggaggaacat ggagtggaac gggaataacc aatccctccg gagtattttc
     4681 tgcatcagtt tccggagtag gcgcgcatgt gttgacgtat tctttcactg acggaaacgg
     4741 atgtacaaat actgatcaac tcgatgccac tatcaatgca cttcctgttg taaatgcagg
     4801 agccgatacg accgtgtgtg atttaccaat acccgttacg cttaccggaa ctcctgtagg
     4861 tggtacatgg agtggtgcca atattactgc cggaggagtt tttactccca acggaacagg
     4921 cgcatttacc gttacctata ctttcacttt aggaacaggt tgtacagcct ccgatgatcg
     4981 cgtggtgaat gttattgcac cacaacaagc caatgccggt cccgatcagg aaatttgtca
     5041 caatgccgct gccattacca ttaacggttt accagccggt ggaacatgga gcggaagttt
     5101 tgtaacagca ggaggattgt ttactccttc cactccgggt acatataatc tcacctatac
     5161 attcggtgtt ggaaattgcc agactactga tgtgatgcag attattgtgc atcctcttcc
     5221 ggttgtaaat gccggggtag atcagaacat ttgcattgat gcagcagtaa ctatattaac
     5281 cggaacacct gttggcggtt catggtcggg aattggaatt accaatgcag ccctcgggac
     5341 ttttgatcct tttgttgccg gagttggtgc ccataacatc acgtatacat ttacgcatcc
     5401 tgttacaggt tgtttgaatt cggatgtgat ggtaatgaat gtacaaccac ttccggtaat
     5461 gggcttcagt aataatccga ttgcctgtgt tgggatcgcc gaaccattta caaatacaac
     5521 aacaggtgct atttcctatg cctggaattt tggtgacgga aatacatcaa cacaaaccaa
     5581 tccgcaaaat gcttatgctg ctgttggaac atacactatt tcgcttactg caacgtcctt
     5641 gttcggttgt gtgaacagca taacaggaag tattgatgta ttcgaaccac cggttgccaa
     5701 tttcaccctt gcgcccgatt cggcttgcgg tccattggtg gtgaacttta ataatatttc
     5761 ttccggacaa tcgatttcct acaactggga ttttggaaac ggaacgactt ctgcactggc
     5821 tgctccgggt gcggtgactt atcctgcagg gtatttagca gatactactt acacgattgt
     5881 attgagtgtt tccaattttt gcgcaacagt aactcatacg gagcaagtca tagtaatgcc
     5941 cgaacccaaa gcgatttttg gaacgaatgt caatgtggga tgttcacctt tcacacctga
     6001 aatcgcaaac aacagtgtgg gattacccga tacctatttc tggaatttcg gtgatggtac
     6061 aacagggaat caagcggatg cattgtttgg gcatacgttt ttcaccggac tagaagatac
     6121 aacatttacc gtcatgctgg ttgtcaccaa cgagtgtggt gttgatacag cctatcatac
     6181 catcactgta ttgcccaata ctgtgaatgc atttttcaat tcgagcgata ccagcggatg
     6241 cgttcctctt gctgtgaatt ttacacagtt ctccaccgga gccacatttt cgagctggga
     6301 ttttggggac gggaatgtga gcacagcacc caatccttca catacattta cacaggcagg
     6361 aacgtatttg gttcagttgt tcatcaatga tggttgtagt tttgataccg caacgattag
     6421 tattactgtt catccgcaac ctcaaatcga ttttactaca acaccggatt ccgtttgtgc
     6481 aagtcagcct ttccagttca ataacctgag ttcgaatctg gcatctgtca actggaattt
     6541 cggagatggg aatacgagtg tattgacgaa tccgattcac acttatggtt tatcgggtac
     6601 ttacatggta acactcagtg gagtttctac ggtatttggt tgttcaacat ccgttacgcg
     6661 tccggtaatt gtacgaacaa ctcctgttgc atcatttgtt cctgatccgc tttttggttg
     6721 tgagcctttg ccggtaagtt tccagaatac aagtacgcaa gcgcaatttt tctcctggaa
     6781 ttttggtgat ggaaacagct cggtgcaggt gaatccttcg catacctatg ccacgtcagg
     6841 aacgtatacg ataacactga tcgcaaccaa cctgaacgga tgttcagata ccgcagtgca
     6901 aagtgtattg gtgaatccga atccgatcgc agcattcagt acgtcttcgc cttcatcctg
     6961 ttattctccg gttactgtaa atatcactaa cctttcttcg ggtgcagtag gttattcctg
     7021 gaatcttggt aacggacaaa caagcgcgct gaccaatccc agcgtgacct atacgcaacc
     7081 cggaagttat acgatcactc tcattgcaga aacgccattc ggatgtctgg atacggcaac
     7141 gtatgtgtat gtagtgtacc caacaccggt agcaaatttc agtattcctt ccgatacttc
     7201 ctgtgaattg tatcctgttc cttttacgaa tctttcgcaa aatgcaacat cgtaccaatg
     7261 gagttttggt gatggttcaa catccgctca gcaaaatccg gttcacaatt atgccgattc
     7321 cggattgtat tcggttacgc tgatggtata tggtgcagga ggttgtggtg atactattac
     7381 gcttaacaat acgctaacaa tttttccttc tccaacagca ggatttacat ggatcaatgt
     7441 tcagaatgtt gatccgctca gcggaacggt tgagtttacg aatacatcaa caggagcaat
     7501 aggatacgaa tggcattttg gtaatggtca aacctccatt gaggtaaatc ctgttcaccg
     7561 gtacaatcgt tacggcaatt ggtcggctta cctcgttgca acaaatgaat atggttgtat
     7621 ggatacagcc taccagaatg tggtggttga tttctttaaa ggtttatttg caccgaacgc
     7681 aatgtatccc gggcatcctg attttggagt tgctcatttt attccaacag gagtcggatt
     7741 aaaatcgttc cgtgtttcca tttatgatga ttgggggaat ttgatttggg agtgttcaga
     7801 tattgttgat acacgtcctg cctgtgcatg ggatggaacc tttaatggcg ctcccgtgca
     7861 gcaagatgct tatgtgtgga aaattgaagc gtttttcctc gatgaatcgc tttgggtggg
     7921 acaactctat cccaacggac tgtaccgtgc tgcaggaact gtaactgtga tacgatgatg
     7981 aaatttttca aactattttt cgcattacta ttgatccatg ccgatgtatt cggtcaggat
     8041 ccgaattttt cgcagttttt tcataatccc atttattaca atcctgcgaa tgttgcattg
     8101 gataatggtt tacgtgtgcg cggaaatgtt cgcaatcagt ggggacctat tcccggtcgt
     8161 ttcaatacgg gatgtttttc gatggatgca gcaggaatca ataaaacggg tttgggcatt
     8221 attgcattaa gcaataacga aggtgagggt cgtttacgca ccatgcaggc aggattgatg
     8281 tacagttatc gtccggttga aacaaaaaat ttcaggttac aggctggatt tggagcttcg
     8341 tttgtgcaga agcgaattga ctggagtcgt tttgtttttt ctgatcagct cgatgaagta
     8401 ttcggaaatg tgagcgctac ttcatttatt ccacccggca ccgaccgtgt tgtgtatcct
     8461 gatttcaatg caggagtcag tgcgcgtttt aacagcggaa aagtggagga aaaaggagga
     8521 aagatgacta caacaattgg cgctgggttt catcatctaa cacgacccaa agacgcattt
     8581 gtttcggatg gaaacagatt gcctttaaaa atggtgatac acggccattc gaatattttg
     8641 tcgtacaacg gagtaattta ttcacccgga tttatttacg agcgtcagtc gaatttcacc
     8701 acgtttcagg taggaataaa tatgtacaag aatcctgtat acgcaggttt ttggtttcgg
     8761 aacagaaatt taaatatgca gggcaagaat tatgattcct ttgttttcgt gttgggcttg
     8821 aattcgttta tggcgcatca acgcagagca aaattctgtt acagttatga ctttaccatt
     8881 tcacgattga aaacttccag ttttggtaca catgaattct ctgtggtttg ggaattgaat
     8941 gatattgtaa cctttagccg aatagaaaaa aacaaacgca agcggcaaaa gacccgtttt
     9001 ctggagtgcg tggattttta aattagttga taggaatttc gatcaatgct ttaaaatcgg
     9061 aggtaagttg ttcctgaata tccgcaaaag gaaccgaaaa cgcccagctt cccatgatgt
     9121 atggtcctat ttcatagttg ttgtacaaaa aggtaatgga tcggtcgctg atgtagaaat
     9181 tagtgttgat gataaatcct tcatcaccga attcgaatcc ctgttcagtg agatctgttt
     9241 ccggactaat tccattaaat gatcgaaaat gtttctcgca gatttttacg aaggcaaccg
     9301 aatcttttac gatctcgctg atcgtgattt tttttccatt cgacttataa aacgatccat
     9361 aggatgtaaa gctgttgggg tgcgcacctc cagtgtagga gctgccggaa acgtacagtg
     9421 aaatgagtcc tttgttttca aaatttattc ctgcaatatt ttcaaccgac catggcatca
     9481 tatcatcacc tattgcattc ttgtcgtttt taaacaaggt gatcaggtat tctgcaaatt
     9541 ctttttgact tctgacattt cctttttcat ccggagtggt gatctgatcc cgtatccagg
     9601 tattcaatgg tgttccggct gaatgtgttt ctgcaaaaac agggtattca aaattaacgg
     9661 tgatgcagga ttgatccggt ttatcacaaa caccttcttt tagttcaatt ttttccgttg
     9721 taaaagcaat cgaatcaact gtggaaatca gtgaatccat
//
LOCUS       DAOMYG010000056         9311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2473_length_9311_cov_8.850475, whole genome shotgun sequence.
ACCESSION   DAOMYG010000056 DAOMYG010000000
VERSION     DAOMYG010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 9311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9311
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2473_length_9311_cov_8.850475"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..325)
                     /locus_tag="PK610_02995"
     tRNA            complement(253..325)
                     /locus_tag="PK610_02995"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(290..292),aa:Phe,seq:gaa)
     gene            390..1148
                     /locus_tag="PK610_03000"
     CDS             390..1148
                     /locus_tag="PK610_03000"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRJ34921.1"
                     /translation="MFKKRDCVLLEVHSVELVLDLGNTALKAGIFLEGKFSHAERIPL
                     EEAPGYINSLSRNYTFGNCIFSSVSSKKDDFDWPKDTNILDFNSKTPVPVKNGYGSPL
                     TLGRDRLANACFAVSSFPGQNVLIIDAGTCLKFDLVTADGTYRGGSISPGLGMRYKAM
                     HTFTGRLPLLSTGAAKTVIGTNTEESMRSGAFMGMVFELKGTMQWYLEKYPDIKFCIT
                     GGDASLLAEDLNFPIFADPYLTLKGLYSILKHNA"
     gene            1141..2544
                     /locus_tag="PK610_03005"
     CDS             1141..2544
                     /locus_tag="PK610_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34922.1"
                     /translation="MRKTTLFFFLIVSLLFLNASAQQNTSSPYSRYAFGELNTNPLAQ
                     YNGMGNASIAVADSFLLNLSNPASFSNLMIHRPIFDIGVGGQRLQVRSEDVTEYNGSF
                     GLRNITLGIPISRRAGFSFGILPFSSVGYTISQTVAEPSIGNVTYTYTGTGGINRLYL
                     GAGYKILSKKNSFLSLGLRGSYLFGNIQRVRKAVYPGYTGIMHTKIRHNTIVSDFLFD
                     GGLLYKAKVFDNSTKKDSIKSNKPKPPGAKHFLSLGLTYTSSSQIGAKQEMIAHTFSS
                     IYFETPVDTVMYADTISGYMNFPQRIGFGFAYDIHKESDGGRIRRYMFSGQYEQQTWS
                     TYSEVFGNNTVQDQLRNSRSIGLGFQFTPDVSPYLRAKSRIWELTNYRMGLRYSDTYL
                     QLNNNQLKQYGISFGLGIPLTNSTKSLSMINFGCEVGRKGTTENQLLQENFFNFFVGF
                     SISPAIHEEWFHKRKYD"
     gene            2547..3890
                     /locus_tag="PK610_03010"
     CDS             2547..3890
                     /locus_tag="PK610_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34923.1"
                     /translation="MKTAITNSMKHIKIIALFAILAGSVFSANAQKEDWGTDSLECKK
                     CLSLYNEPLKNKNYAEALIHWRGVIRVCPKYKESVYINGAIIYRSIIDAEKDAAVKDK
                     LIDTLAWVYEKRMEYFGRSNEALESYGNDMIRYRQSTPSVAYKIIKELVDREQGKASC
                     AALMRYYQSLVLMYRKKEPGIDNNKMVEEYFKTKELLEQSIGNNPNDKNCTLANEEVD
                     KYGMPFLPCDKIYEYAKTKFNDVPKDNKEVRLNEMKKLLSVMNKRNCNDNEMYDKILA
                     EVLEAEPSADGFYSLGLSMNNKKKYAEANTNFKKAIELCGDCEKNGEYLLGAAKANLN
                     SGNYSTAASFARQAMAKDSKQAGQAYLIIAQAIARSGCGNNSFEQKFNYVLAVEYCQK
                     AKAADGSVADEANRLIGSYRGNFPTKTELFDRSMHDKESFQIGCWINESVRIPKD"
     gene            3890..4468
                     /gene="lptC"
                     /locus_tag="PK610_03015"
     CDS             3890..4468
                     /gene="lptC"
                     /locus_tag="PK610_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRJ34924.1"
                     /translation="MQERSSVRSRILLIPALIGAGMFFSCENDLQEVDRVARLGEIPQ
                     ETMEEVRLVYTDSGETRAILETPLIHKFYQPRERMEFPNGLQLTFYQKGMKMESRLTS
                     EYGILNQAERHLLVRNNVVFINFIRQDTLNTELLNWSQDSARVYTDKQVVVRGKTGVF
                     TSRGGLQAGEAFDWYEFNGVAATYYYNSKTDE"
     gene            4481..4702
                     /locus_tag="PK610_03020"
     CDS             4481..4702
                     /locus_tag="PK610_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34925.1"
                     /translation="MNKYYRFSEIMWLLLTITVTIMVVVLMISEESIEKSKWYLIIPL
                     LSAAMYIMRRSLRIRFEKNQSEGNAKGKK"
     gene            4705..5970
                     /locus_tag="PK610_03025"
     CDS             4705..5970
                     /locus_tag="PK610_03025"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRJ34926.1"
                     /translation="MDSTLFFYILITILFSAFFSGIEIAFITANKLRIELENKQGKFP
                     ARILSFFVRKPSRFIGAMLLGNNIALVMYGIFMAALLEPGIEFYITENRWLVLLIQTI
                     TSTIIILFTAEFLPKALFRIDPHRVLNLLAVPTALIYFILWIPMIILIGISEGILRIF
                     ARDNSGDGTDVGLGRIDLDHYLEEVTQSAAKNNHEIDQEVQIFRNALDFSNIKVRDCM
                     VPRTEMIAINVDSEIEALRSLFVETQLSKIFVYRDSIDNIIGYVHSYELFKQPQSIQN
                     ILLPVAFVPESQQAQTVLEEFTRQSRSIAVVVDEFGGTSGLVTIEDIIEEIFGEIEDE
                     HDTEEHTEQRINENEFLFAGRLEIDYLNETYQLNIPYSDEYETLAGYLINHLQDIPEK
                     YTRTTIGKYDFVVESVSGQRIETVRVIKS"
     gene            6176..8281
                     /locus_tag="PK610_03030"
     CDS             6176..8281
                     /locus_tag="PK610_03030"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRJ34927.1"
                     /translation="MAIIGKIRENSVLVLIIVGISLVLFILGDFLTNPGNSAGDNSVG
                     SAYGESIDAMEYDSRVNQEIDAEKQNRAMRGMPELNENDMDQIRENVFNSMVADIIRN
                     RELSHFPFSVSSEELNDMVHGVNLHPAIRQEPGFQGPDGQFSRDSLIRYLNYLESPTD
                     QAREMKRRWKQFEQDLKRDRIGIKYSTLITKGVYVTNAEAKRDYEDNNRTYKIRFVSK
                     RYFEIPDSMVTVTDEDIRAYYDKNKFRKQWEQQGSRVFEYVQIPLVPSEEDRAKTREE
                     LNNLKELFRESKNDSSFVMSYSENKYFADRFYNGSELSPGVDSLIQKLDSGDVVGPYE
                     EFGYMRIGKIRGVKFETEARIRHILLSKDKGDIATLNKRADSIKNVIKRNKNFEEMVT
                     KFTDDPGSVNNGGVYEWFGKGVMVEPFEKACLNGKTGDLVSVETSYGVHIIEILGKRE
                     AKRVKYATVDIKIVPGPETEATARGIAMDFLETIPDGTKFEESAQKANMVVLRDEIPA
                     NQRTMNQDEKSRELVRFVQTGMEHDVSEPILLGDFLIVAHISAVKPKGVPEFEDVKEM
                     MAVNARQEKKAEMLKKQMTGAKSLEEIATRVGGTVLEADVTFATPTIRGGGGNEYNVV
                     GTLFSMTAADKGAITVPISGKVGVYVVQLVDIVEPQPAADLSNNKRALGSLRRNRAGG
                     DAFNALKENAKVVDNRQLF"
     gene            8427..8627
                     /locus_tag="PK610_03035"
     CDS             8427..8627
                     /locus_tag="PK610_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HRJ34928.1"
                     /translation="MPDDFSGFSRRSNLDPEDYYLSEEGFIIFTEKYLLKRGYCCKSG
                     CKHCPYGYNKKTGKIEGKKGLN"
     gene            complement(8654..9226)
                     /locus_tag="PK610_03040"
     CDS             complement(8654..9226)
                     /locus_tag="PK610_03040"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRJ34929.1"
                     /translation="MKKILIVNAHPLAGSFCDAIVEHFVAGINSENQQVKTVCLRDLK
                     FDPILHGGYRNPTELEPDLRMAQDYISWSDHIVWIFPIWWGEMPALLKGFIDRTFLPG
                     FAFRYKKDSPMWDRLLKGRTTSIITTMDAPVWYHRWIMGGHGIRSMNKFILRFCGLKL
                     NRVTYFGSMRFSTDEQRKKMLEKVSRLASK"
BASE COUNT         2830 a         1985 c         1977 g         2519 t
ORIGIN      
        1 ccgtgctttt ccaaaatcac tatgctcctc cttaaaaatt aaaatccatg gaatatgtcc
       61 agatgtaaat tttgatttcc ccgaattgtg ctctttaaac cggcgatcaa aatcctctgt
      121 aattccaaca tacaacagtc cggatgatag actcttcaat acataaacgg tcaccataat
      181 cctgctcttt caatgaaaat tatttcataa aaaaacccgg tacgaaatac cgggttcctt
      241 tgtcttgttc tgtggtgtag gagggaatcg aacccccgac acaaggattt tcagtccttt
      301 gctctaccaa ctgagctact gcaccatcac taccttgcat tgatcaaggg tcggcaaaaa
      361 tagaaaaaat ttggcaccag caaaaatcaa tgttcaaaaa gagggattgt gtacttttgg
      421 aagtgcattc agtagaactt gtactagatc tcggcaatac cgctctaaaa gcaggaatct
      481 ttttagaggg aaaattttcg catgcggaac gcatcccgct ggaggaggca ccaggctata
      541 tcaactcttt atcaaggaat tacacctttg gcaactgcat cttttcaagt gtttcttcaa
      601 agaaagatga ttttgactgg ccaaaagaca ctaacatact ggatttcaac tcaaaaacac
      661 cggtacctgt gaagaatggg tacgggagtc cactaacgct tggtcgcgac cgactggcta
      721 atgcttgttt tgctgtcagc tcttttcccg gtcaaaacgt tttgattatt gatgccggaa
      781 cctgcctgaa atttgatctg gttactgctg acggaactta tcgtggcggc tccatatcgc
      841 ccggtttggg tatgcgctac aaagccatgc atacttttac aggacgttta ccgttgttat
      901 ctaccggagc tgcaaagacg gtgattggca caaacactga agaatcaatg cgatccggag
      961 catttatggg aatggttttt gagctaaaag gaacaatgca gtggtatttg gagaagtatc
     1021 cggatatcaa attttgtatc acgggaggcg atgcttctct tttggcggaa gaccttaatt
     1081 ttcccatctt tgccgaccct tatctgaccc taaaaggtct ttattcgatt ttaaaacaca
     1141 atgcgtaaaa caacgctttt cttttttttg attgtcagtt tgctttttct gaatgcaagt
     1201 gcacaacaaa acacttcatc tccttactcg cgctacgcat ttggagagtt aaataccaac
     1261 ccgcttgcgc aatacaacgg aatgggaaat gcttccattg cggtggctga ttctttccta
     1321 ttaaacctca gcaatccggc atccttttct aacctgatga tacatcgtcc gattttcgat
     1381 attggtgttg gcggacaacg tttacaggta cgttcagaag atgtgactga atacaacggt
     1441 tctttcggtt tacgtaacat aacactcgga attcctatta gcaggagagc aggttttagt
     1501 ttcggaattc ttccattcag ttcagtaggc tataccatca gccagaccgt agccgaacca
     1561 tccattggaa atgttaccta cacttatacc ggaaccggcg gaataaatcg cttgtatctt
     1621 ggtgcaggat acaaaatcct gagtaagaaa aacagttttt tgagtttagg tctccgtgga
     1681 tcttaccttt ttggaaacat acaacgcgta agaaaagcgg tttacccggg ttataccggc
     1741 attatgcaca ccaaaatcag acacaatact attgtaagtg attttctatt tgatggtggt
     1801 ttgctgtata aagcaaaagt gtttgacaat tccaccaaga aagactctat aaaaagcaat
     1861 aaaccaaaac ccccgggagc aaaacatttt ttgtcacttg gtttaaccta tacttcttcc
     1921 tctcaaattg gtgccaaaca ggaaatgatt gcacatacct tttccagcat ctattttgaa
     1981 actcctgttg acacggtaat gtatgccgat accatttcag gatatatgaa tttcccgcaa
     2041 cgtatcggtt ttgggtttgc ctatgatatt cacaaagaaa gtgatggcgg aagaattcga
     2101 aggtatatgt tcagtggtca gtatgaacaa caaacctggt cgacttattc cgaagttttt
     2161 gggaacaaca ctgttcagga tcaacttcgg aacagtcgca gtatcggact tggatttcag
     2221 ttcacacctg atgtttcacc ctatctccgt gcgaagtcaa ggatttggga attaacgaat
     2281 taccgaatgg gtttgcgtta cagcgacact tatttacaat taaataacaa ccagcttaaa
     2341 cagtatggca taagttttgg attaggcata ccgctaacca attctactaa atcgctttcg
     2401 atgatcaact ttggttgtga ggtcggaaga aaaggcacta ccgaaaacca gttgttgcag
     2461 gaaaatttct tcaacttctt tgtcgggttc tcgatatcgc cggcgattca tgaagaatgg
     2521 ttccataaac gtaaatacga ttaacaatga aaaccgcaat aaccaatagc atgaaacaca
     2581 tcaagatcat cgctctcttt gcgatacttg ccggaagcgt tttttccgcc aatgcacaaa
     2641 aagaagactg gggaaccgac agcctggagt gtaagaaatg tctttcgctg tacaatgagc
     2701 ctctgaaaaa caaaaattac gctgaagcac tcattcactg gagaggtgtt atacgcgttt
     2761 gtccaaaata caaagaatca gtttacatca atggtgcaat catttaccgc agtatcatcg
     2821 acgcagaaaa agatgcagca gtaaaagata agcttatcga tacccttgct tgggtgtacg
     2881 aaaagcgcat ggaatatttt ggccgtagca atgaagcgct tgaatcctat ggaaacgaca
     2941 tgattcgtta ccgtcaaagc accccttctg ttgcttataa aatcattaaa gaactggtag
     3001 atcgcgaaca aggaaaagca tcttgtgctg cgctgatgcg ttactaccaa tcactggtgt
     3061 tgatgtaccg taaaaaagaa cccggtatcg acaacaacaa aatggtggaa gagtatttta
     3121 aaacaaaaga attactcgaa caatcgattg gcaacaatcc gaacgataaa aactgtacgc
     3181 ttgccaacga agaggtagac aaatacggaa tgccctttct gccttgcgat aaaatttacg
     3241 aatacgccaa aaccaaattc aacgatgttc cgaaagacaa caaagaagtt cgtctaaacg
     3301 aaatgaaaaa actgttgagc gtaatgaaca agcgtaattg caacgacaat gaaatgtacg
     3361 ataagattct tgctgaagtt ctggaagcgg aaccttctgc agatggattc tacagccttg
     3421 gtctgagcat gaacaacaag aaaaagtacg ctgaggcgaa caccaatttc aaaaaggcaa
     3481 ttgagttatg cggagattgt gaaaagaacg gcgaatacct gttgggagct gcgaaggcaa
     3541 atcttaactc cggaaactac tctaccgctg caagttttgc acgtcaggca atggcaaaag
     3601 acagcaaaca ggcaggccag gcgtacctta tcattgcaca ggctattgca cgttccggtt
     3661 gcggaaataa ttcattcgaa cagaaattca attatgtcct cgctgttgag tattgccaaa
     3721 aagctaaagc agcagacggt tctgttgctg atgaagccaa tcgcctgatt ggttcgtaca
     3781 gaggtaattt cccaaccaaa accgaacttt tcgaccgcag tatgcacgac aaagaatcgt
     3841 tccagattgg ttgctggatt aacgaaagtg tacgcattcc gaaagactaa tgcaggaacg
     3901 atcgtctgtc cgatccagaa tacttctcat tccggccctt atcggggccg gaatgttttt
     3961 ttcgtgcgag aacgatctgc aggaagtaga ccgtgttgca cgtttaggag aaattccaca
     4021 ggaaacgatg gaggaagtgc gactcgttta caccgattcc ggcgagaccc gagcaattct
     4081 ggaaactccc cttatccata aattctacca gccccgcgaa aggatggaat tccccaatgg
     4141 tctgcaactc accttttacc aaaaaggaat gaagatggaa tcgaggttga catccgaata
     4201 tggaatacta aatcaggccg aaaggcattt gttggtcagg aacaatgttg tttttattaa
     4261 ttttatcagg caggacactc taaacacaga actactgaac tggagtcagg acagtgcccg
     4321 ggtgtatacc gacaagcaag tggtggttcg cggaaagacc ggagttttca ccagcagagg
     4381 cggtttacag gctggtgaag cgttcgactg gtacgagttc aacggtgtag ctgcaacgta
     4441 ttattacaat tcaaaaacgg acgagtaagt ccaaatgaac atgaataaat attaccgatt
     4501 ttctgagatc atgtggttgt tgcttacaat aaccgtaaca ataatggttg ttgttcttat
     4561 gatctctgag gaaagcattg aaaaatcgaa atggtacctg atcatacccc tgttgagtgc
     4621 ggccatgtat attatgcgtc gcagtttgcg aatcaggttt gaaaaaaacc agagcgaggg
     4681 caatgctaaa ggcaagaaat aaaaatggat tccaccctct ttttttacat tctcattacc
     4741 atcctttttt ccgccttctt ttcgggaatt gaaattgcat ttataactgc aaacaaactt
     4801 cgaattgagc ttgaaaacaa acaaggaaaa ttccctgcgc gcattctaag ttttttcgtc
     4861 cgaaaaccat cacgtttcat tggtgcaatg ttgttgggta ataatattgc tttggtgatg
     4921 tacggtattt tcatggctgc attattggag cccggtattg aattctacat caccgaaaac
     4981 cgctggttgg tattactgat ccaaacgatc acctccacca tcatcattct gtttacggca
     5041 gaattcttac ctaaagcatt attccgaatt gatccccacc gcgttctcaa tctgcttgct
     5101 gttccaacag cattgatcta tttcatacta tggattccta tgataatcct gattggtatt
     5161 tcagagggaa ttctacggat ttttgcacgc gataatagtg gtgatggcac cgatgttggt
     5221 ttgggcagaa tcgatctgga tcattatctg gaagaagtga cacaaagtgc tgccaaaaac
     5281 aatcacgaga ttgatcagga ggttcagata ttccgcaatg cacttgattt ttccaatatc
     5341 aaagtacgcg attgcatggt accccgcacc gaaatgatag cgattaatgt ggattcagaa
     5401 attgaagcac ttcgatcgct ctttgtggaa acacagcttt caaaaatctt tgtgtatcgc
     5461 gacagcatcg ataatattat cgggtatgtg cattcctacg aattgttcaa acaacctcaa
     5521 agcattcaga acattctcct gcctgtagca tttgttcccg aatcgcaaca agcacaaact
     5581 gtattggaag aattcacccg tcaaagccga agcatcgctg ttgttgtgga tgaatttggc
     5641 ggaacatccg gactagtcac tatcgaagac atcattgaag aaatttttgg tgaaattgaa
     5701 gacgaacacg ataccgaaga acatacagaa caacgaatca atgaaaacga atttttgttc
     5761 gccggaaggc ttgaaattga ttacctcaat gaaacctatc agctgaatat tccctatagc
     5821 gatgaatacg aaacccttgc cggctatttg atcaaccatt tacaggacat ccccgagaaa
     5881 tacacccgta ccaccatcgg caaatacgac ttcgtggttg aatcggttag cggtcagcgc
     5941 attgaaaccg ttcgggtgat aaaatcctaa ttggcatttc cgtttagaga tctaaattct
     6001 ctttatttct gaaattctta tataaacatt taataatcag ttgtttattc acccaattct
     6061 tgaagaaaaa agcatttgta gatgaactga aaatcaaatc attctgccgg gctgattttc
     6121 aattttcatt taaggattct cctgcttata ttcgcagccc taaattccca ctacaatggc
     6181 catcattggt aaaattcgcg aaaactcagt actggtactt atcatcgtag gtatttcgct
     6241 tgtattgttc attcttggtg acttcctcac caatcccggc aattcggcag gagataattc
     6301 tgttggttct gcttacggag aaagcatcga tgcgatggaa tacgatagtc gtgttaacca
     6361 ggaaatagat gcagaaaagc aaaaccgtgc aatgcgcggc atgcctgaac tgaacgaaaa
     6421 cgacatggat cagatccgtg aaaacgtatt caacagcatg gttgcagata tcatccgtaa
     6481 ccgtgagctg tcgcatttcc ctttttcagt ttcgtctgaa gaattgaacg acatggttca
     6541 tggtgtaaac ctccatcccg ctattcgtca ggagccgggc tttcagggac cggacgggca
     6601 attctcacgc gactcgctga ttcgttacct caactacctc gaatcgccaa ccgaccaggc
     6661 acgtgaaatg aaacgtcgct ggaaacagtt tgagcaggac cttaagcgcg atcgcatcgg
     6721 aattaaatat tctacattga tcaccaaagg tgtttatgtt accaacgcag aagccaagcg
     6781 cgactacgaa gacaataacc gcacttacaa gatccgtttt gtttcgaagc gttattttga
     6841 gattccggat tcaatggtta ctgttaccga cgaagatatc cgtgcttact acgataaaaa
     6901 caaattccgt aagcagtggg aacaacaggg ctcacgcgtg ttcgaatatg ttcaaattcc
     6961 tttagtaccc tctgaagaag atcgcgccaa gaccagagaa gagctgaaca acctgaaaga
     7021 actattccgc gaatcgaaaa atgattcatc gtttgtgatg agttattcag aaaacaaata
     7081 tttcgcagat cgtttctata atggcagtga gttaagtccc ggcgttgatt cactcatcca
     7141 aaaactggat tcgggtgatg ttgttggtcc ttacgaagaa tttggctaca tgcgaatcgg
     7201 aaagatccgc ggggttaaat tcgaaaccga agcgcgtatt cgtcacattt tgctgagcaa
     7261 agacaaaggc gatattgcca cattgaacaa gcgtgccgat tcaattaaaa atgtgatcaa
     7321 gcgcaacaag aatttcgaag aaatggtaac caaatttacc gacgatccgg gcagcgttaa
     7381 caatggcggt gtttacgaat ggtttggtaa aggagttatg gttgaaccct tcgaaaaagc
     7441 gtgtctgaat ggaaaaaccg gagatcttgt ttctgttgaa acttcttacg gagtgcacat
     7501 catcgagatt ctcggaaagc gtgaagcaaa gcgcgttaaa tatgcaactg tagatatcaa
     7561 gattgttccc ggtccggaaa ccgaagcaac agctcgcggc attgcaatgg atttccttga
     7621 aacgattccc gacggaacaa aatttgaaga atctgcacag aaggccaaca tggttgtatt
     7681 gcgcgatgaa attcctgcaa accaacgcac catgaatcag gacgagaaaa gccgcgaatt
     7741 ggttcgcttt gtacagacag gcatggagca tgatgtctct gaacctattc ttttaggcga
     7801 tttcctcatc gttgcgcata tcagtgcagt aaagccaaaa ggtgtacctg aatttgaaga
     7861 cgtaaaagaa atgatggcag taaatgcacg tcaggaaaag aaagctgaaa tgctgaagaa
     7921 gcaaatgaca ggcgcaaaat cacttgaaga gattgctacc cgtgtaggcg gaactgttct
     7981 tgaggccgat gttacttttg ccacacctac cattcgtggt ggtggcggaa acgaatacaa
     8041 tgttgttgga acgctgttct ccatgaccgc tgccgataaa ggagctatta ctgttccgat
     8101 cagtggtaaa gttggagtgt atgtggttca attggttgat attgttgaac cacaacctgc
     8161 agccgatctt tccaataaca aaagagcact aggaagtctg cgccgcaacc gtgccggtgg
     8221 agatgctttc aatgcattga aagaaaatgc caaagtggtg gacaaccgcc aactgtttta
     8281 attaatactt gctttttgtt aaagacccgc aattgcgggt cttttttttt ctgttcaggt
     8341 agtacgcgca gcaaaattcc attctaacaa aatatccaaa aatcattcgc atttttgcat
     8401 agttaagctc ccaacaagcc cccaacatgc ccgacgattt ctccggattt tcacgacgaa
     8461 gtaatcttga tcctgaagat tattatttaa gcgaagaggg ctttatcatt tttacagaga
     8521 agtacctgtt aaaacgcggc tattgctgca aaagcggatg caagcattgt ccgtatggct
     8581 acaacaaaaa aaccggcaaa attgaaggca agaaaggctt aaactaaagc ccggaatcat
     8641 tctccccctt actctacttt gaagccaggc gactcacttt ttcaagcatt ttctttcgct
     8701 gttcatctgt actaaaacgc atagacccga aataagtgac cctgttcaat ttcaatccgc
     8761 aaaagcgaag aataaattta ttcattgaac gaattccatg gccccccata atccagcggt
     8821 ggtaccaaac aggcgcatcc atagtagtga tgatagatgt agttctgcct ttaagtaacc
     8881 gatcccacat tggtgaatcc tttttatagc ggaatgcaaa accgggaaga aaagtgcgat
     8941 cgataaagcc ctttaataat gcaggcattt cgccccacca aatgggaaat atccatacaa
     9001 tgtggtcgga ccagcttata taatcctggg ccattcgcag atccggctcc aattcggttg
     9061 ggttgcggta tcctccatga aggataggat caaatttcag atcgcgcaga caaactgttt
     9121 tcacctgctg attctctgaa ttgattcctg caacaaaatg ctcaacaatg gcatcacaaa
     9181 aacttcctgc aagaggatgt gcattaacta ttaggatctt tttcataatg atttgttttg
     9241 gaaatggctt gaattaaaat gtccagcgga acatcggtac agggaaaaac ccttgctgat
     9301 attgtgtcac c
//
LOCUS       DAOMYG010000057         9274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2488_length_9274_cov_10.212713, whole genome shotgun sequence.
ACCESSION   DAOMYG010000057 DAOMYG010000000
VERSION     DAOMYG010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 9274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9274
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2488_length_9274_cov_10.212713"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1262
                     /locus_tag="PK610_03045"
     CDS             162..1262
                     /locus_tag="PK610_03045"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRJ34930.1"
                     /translation="MKVHLDLLPLQDWLPGLERPLFISGPCSAESEEQVRATAEAIAQ
                     SGKVKVLRAGIWKPRTRPDTFEGKGEQALPWLKNAAKQYGMYSATEVANAAHAEAALK
                     AGIDMLWIGARTTVNPFSVQEIADAIRGVDIPVLVKNPINPDLQLWIGALERVNRAGI
                     KKLGAIHRGFSSFEKSPFRNAPMWEIAIQLRTLLPELTLICDPSHIAGNRELLPLVAQ
                     KAIDLDMAGLMIEAHIDPSVALSDAQQQLKPEALLGLLSELNIRSASVNDPVFLNLLE
                     EMRKAIDELDDDILQRLAARMQIAEKIGEYKRDNQVTILQVQRWDEIIGKRLFQGKAM
                     GLSEEFVRKLLELVHQESIRRQTGVMNSKLTK"
     gene            complement(1322..1573)
                     /gene="purS"
                     /locus_tag="PK610_03050"
     CDS             complement(1322..1573)
                     /gene="purS"
                     /locus_tag="PK610_03050"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HRJ34931.1"
                     /translation="MKFKAEIDVMPHDALLDPQGKAVSNSMKNLNLSQISNVRIGKHM
                     TLNVDASNKEEAGKLVDEACKKLLHNPIMESYRFTISEQ"
     gene            complement(1557..2372)
                     /gene="pssA"
                     /locus_tag="PK610_03055"
     CDS             complement(1557..2372)
                     /gene="pssA"
                     /locus_tag="PK610_03055"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRJ34932.1"
                     /translation="MQLKKHIPNLFTLGNMLCGCLAIIACFSWDFQLSALLIFLGAVF
                     DFFDGFLARLLKVSGDLGKQLDSLADMVTFGLAPGIIVFQLLRFSLWTKDVNIDSLYP
                     MGAFIAMLIPLLSGYRLAKFNIDTRQSDSFIGLPTPANALFFSSIAYFLDLKSLAKSY
                     EFALLQFHQTPSYKLNFSETTFTSLEIFLFSPGFLISSTIIFSFLLIAELPLLALKFK
                     QFGFKGNEWRYSLLGLSALLLIIFQVSAIPIIVVLYILISILNNTIKSHEIQS"
     gene            2470..3546
                     /locus_tag="PK610_03060"
     CDS             2470..3546
                     /locus_tag="PK610_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRJ34933.1"
                     /translation="MRHLLLSFILLSSSSALFAQAPKYSNEFLSIGVGARSLGMSGSV
                     VGSTEDVTSGYWNPAGLNGIQSDMQVGLMHSEYFAGIAKFDYGSMAKRIDSSSVFGVS
                     IVRFGIDDIPNTIELIDPSGNINYDRISTFSAADYAFMFHYARKTAIPGLQLGATAKI
                     VHRRIGDFARSWGFGIDVAAQYRKGDWRFGAVARDITTTFNAWSYSLSDEMITTFLQT
                     GNEIPTNSIELTLPKLLLGAGRMFRIKENFSVLGEFGFDCSFDGKRNTLIKSNFISID
                     PHFGFEAGYKNIVFLRGGMGNVQRSMRIDQSGNETTFQPNIGIGVRIKRFAIDYALTD
                     IGDQSVAQYSNVFSLRFDIHKAGS"
     gene            3543..8732
                     /locus_tag="PK610_03065"
     CDS             3543..8732
                     /locus_tag="PK610_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRJ34934.1"
                     /translation="MKKILLLLLMVWSGLSVLGQSAGNEWIQFGGPGKYFVIPVTQSG
                     IHRIDYNTLQNAGLPLGTIDPRNFQVFGREKEQFIFVEGESDGSFDPGDFIEFYAEKN
                     DGSVDSLLYVGGRSSMPDTYYSLYNDTICYYLTWNSSLANRRMPQLNSAAYASFTPSD
                     WFWSEVYKKVALRYYQGPQIVGASSSHFLSGEGWYNDLLYASSGSSQIDLLPTSFPYQ
                     GAGAPDAFAKFVFSGNSNATTFPPLQPNHHMRVGFGPANTLFLDTTYVGYRMIRSDGV
                     FPNALLSGAGSRIQFQTVNDLGVASDIQQVAFSSIRYPRIPDLNTGTALEFFVPDHGS
                     QNHTRIDFQNPPTGNSFVYVLGDTLKRIAPEVNGAFSSVLVPNHFSGTEQRLWFSAES
                     AAISVAQIFPVNGTGDFTDYAQLNPDSVFLIITHSSLMGAAQQYALYRSSPAGGSYQT
                     LVVDVDELYHQYGCGIPKHILGVRRFLADITNEWSTAPSHLFLIGKGIREATESSNGT
                     FPGSRQSLSAYQQNLVPSYGYPSSDQLISAGLNGVFTALPSIPTGRLSASDPQTVLNY
                     LSKMQQYEAAQSPFSSYTIPDKEWQKQIMHFGGGTTTSEQADFSYCLSVYEALLEGVK
                     FGGNVQTFLKQSSDPIDPIEYAEVMQQIQDGVSIMTFFGHSTINGFDQNIDEPSNWNN
                     TGKYPFLIGNACYSGDIFQPGIISASEEFVFEHQKGTIAFLSTSKLGFVPNLHSFSTV
                     FFTRVANVDYGLSVGYILRQTIGQIQPSTVGANLLQENVCTQMTLHGDPALKINYHQA
                     PELVVRQQDVFFNPSTVTLADDSINVNVVVTNIGRATTDTVIVELRRHFPNGSDSIYF
                     KTLNGSFFKDTLTFRIPVLHNIAVGINQFEVLVDIPSFVTEHADETGNNQVMVPLFIS
                     SNGIIPVWPYEYAIVPDSVMDLKASTYNPLLGTRTYRFEIDTTDQFNSPVRRHQLVTA
                     PGGVIIARATNWLNSMSGIASLLEFADSTVYFWRVTPDSSVYSWHESSFQYISGLNGW
                     GQAHFYQFEANHHQTMNYSRASRSWYWDPNLRRIGCDVYSFAANSSEFFGTLWTLDGN
                     QQDYSGCGVDPAIHVAVIDPVTMEPWGTYGCDPVTGGSCPTCIMVNQDRQYGNVNDGC
                     ACRNRVEFYFSFRNTNPVEMDSLLSMVNNHIPDGHYVLLYTWRFNDYSTWTPAHYAMM
                     SALGAGDSIYPGRPNESWIVLARKGDPSFTKVVMGQTGPGFVTMNDTIVGFDFTGAMT
                     SVRVGPSSNWGSLYWKYKSLESPSRDSARISVIGITSSGSETLLFDTLFTPYDSILNL
                     GSLVDASVYPYLRLRGWFTDTTFFTPAQVQRWQLVYTPVPEAAVNGAAGFYLTPGADT
                     IPEGKPFELAIAIENISNQTMDSLLVHYWIEDANRIRHYLTYGRQDSLRPGQILLDTI
                     QIDTRGFSGLNSIWMEVNPVPLLSVNNTYDQLEQLHVNNFAQIPFFVQRDITNPILDV
                     TFDGMHILNGDIVSAKPFILVSLKDENPWMVMNQESDTSFFAVYLTDPTGNQRRIYFR
                     ENGQPVLLWYPSSGAGGKFRIEYQPQLFLDGKYTLLIQSSDKSGNNSGSNDYRIQFEV
                     VNRQTITEVLNYPNPFTTRTHFVFTLTGSELPDQMKIQIMTISGKVVREITMDELGPI
                     RIGRNITEFYWDGTDQFGDRLANGVYLYRVVTRYRGQDVEKRESGADEYFTKGIGKMV
                     LMR"
     gene            8743..>9274
                     /locus_tag="PK610_03070"
     CDS             8743..>9274
                     /locus_tag="PK610_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34935.1"
                     /translation="MIRIEYILLQNDAAFPAEQEFSALFMPGFWKDENKISVDSDASV
                     QFDQPGKWNYLDIELLDDDRITQATFRIDVFRESNYPRSAMFTDADIESLVREYRQGR
                     FNGSAEIKNIAGWCFTSLMLETEDPRMRKEFIQSVDRFLKAAGGIVVYPELMNPETFR
                     SFHSLVQDVPEPEIEPE"
BASE COUNT         2486 a         1972 c         2147 g         2669 t
ORIGIN      
        1 aagtgaaata ctaatcgaat gggtatggaa agtgaaaaga atagcaatga attccgactt
       61 cagttcctgc gaaatagcgg aaaaggagca attcctgctt acctttgtgt tctcagcgaa
      121 ttgctgattc acgctgcaca gattagaaag gaaaaatcca gatgaaagtc cacttagatc
      181 ttcttcctct tcaggattgg ttgcccggtc tggagcgtcc tttatttatt tccggtccct
      241 gcagtgcaga atcggaagaa caggtgaggg ctacagcaga ggccattgcg caatccggta
      301 aagtaaaagt actccgcgcc ggaatctgga aaccgcgtac gcgtccggat acctttgaag
      361 gtaaaggtga acaggctttg ccttggttaa aaaatgcagc gaagcaatac ggaatgtact
      421 cggcaactga agtcgccaat gcagcgcatg ccgaagccgc actgaaggca ggaatcgata
      481 tgttatggat tggtgcgcgg acaacggtca accctttttc tgttcaggaa attgcagatg
      541 ccatacgggg tgtggatatt cctgttctgg tgaaaaaccc gatcaatccc gatctgcaat
      601 tgtggatcgg tgcacttgag cgggtcaacc gtgctggtat aaaaaaactg ggtgccattc
      661 accgtggatt ttcttccttc gagaaatcgc cttttcgcaa tgcgcccatg tgggagattg
      721 ccattcagtt acggaccctc ttacctgagc tcaccctgat ttgcgatccg agtcatattg
      781 caggtaaccg cgaattattg ccattggtgg cgcaaaaagc gatcgatctg gatatggcag
      841 gattgatgat tgaagcacat atcgatccct ctgtagcatt aagcgatgca cagcaacaat
      901 tgaaaccgga agcattactc ggattattat ccgaactgaa tatccgcagc gcatcggtaa
      961 acgatcctgt ctttttgaat ttactggaag agatgcgaaa ggcgattgac gaattggatg
     1021 atgatattct tcagcgcctg gcagcacgta tgcagattgc ggaaaaaata ggagaataca
     1081 agcgcgataa tcaagttacc attctgcagg ttcagcgttg ggatgagatc atcgggaaac
     1141 gtttgttcca gggtaaggcg atgggactga gcgaagagtt tgtgcggaag ttgctggagc
     1201 ttgtgcatca ggaatccatt cgcagacaaa cgggcgtgat gaattcaaag cttacgaaat
     1261 aagcttcgga taaagttcaa tggggaaata aggaattccc tcccgaaata gaattgaagg
     1321 attattgctc gctgatcgta aagcggtagc tttccatgat agggttatgg agcagttttt
     1381 tacacgcttc atccaccagt ttaccggctt cttccttgtt gcttgcatca acgttgagtg
     1441 tcatgtgttt accaatacgc acattgctga tctgcgaaag gttgaggttt ttcatgctgt
     1501 tgcttactgc cttaccttgt gggtcgagaa gagcatcgtg gggcattacg tcgatttcag
     1561 ctttgaattt catgactttt gattgtattg ttgagtatgg aaataaggat gtacaaaact
     1621 acaataatgg gaattgcact gacttgaaat attattaaca acagagcaga tagtcctaac
     1681 agcgaatacc tccattcatt gcctttgaaa ccaaactgtt tgaattttaa ggcgagcaac
     1741 ggtaattcag caatcaatag aaaagaaaaa atgattgttg aggaaatgag aaatccaggg
     1801 gaaaaaagga aaatttctaa ggatgtaaac gtagtttcac tgaaattaag cttgtaagaa
     1861 ggtgtctgat gaaattgtaa aagggcaaat tcatagcttt tggctaaaga tttcaggtcc
     1921 agaaaatagg ctattgatga aaaaaacaag gcattggctg gggtcggtag tccaataaac
     1981 gaatcgcttt ggcgtgtgtc gatgttgaat ttagcgagtc tgtaacctga taacaaaggg
     2041 attagcatag ctatgaaggc tcccattgga taaaggctat caatgttgac atccttggtc
     2101 cataaggaaa aacgcaaaag ctgaaaaaca attattccgg gagcgagtcc aaatgttacc
     2161 atgtcagcaa gcgaatcaag ttgttttcca agatctccgc taacttttaa cagcctggcc
     2221 agaaatccat caaagaaatc gaatactgca cccagaaaaa taagtaatgc tgaaagttga
     2281 aaatcccaac tgaaacatgc aattatagcc aagcacccgc atagcatatt gcctagggtg
     2341 aatagattag ggatatgctt ttttagttgc acatgcaaat aaagcgaaaa ggacacaatt
     2401 ttagctctga ttttgagttt taatgcgaac ttctgaactc cttttttgtt tatttttagc
     2461 gtgtattcta tgcgacactt acttctctct ttcatacttc tctcttcttc gtctgcgctt
     2521 tttgcccagg cgcccaagta ttccaacgaa tttttgtcga tcggtgtggg tgcgcgttcg
     2581 ctggggatgt cgggttctgt ggtgggttca accgaagatg ttaccagcgg atactggaat
     2641 cctgcaggac tgaacggtat tcagagcgat atgcaggtgg gcttgatgca ctccgaatat
     2701 tttgccggaa ttgccaagtt cgattacgga agcatggcta aacgcatcga ttcttcctct
     2761 gtgtttggtg tttccattgt tcgttttggt attgatgata ttccgaatac catcgaactg
     2821 atcgatcctt cggggaatat caattacgat cggatctcta ccttttctgc tgccgattat
     2881 gccttcatgt ttcactacgc acgcaaaacc gccattcccg gattgcaatt gggtgctacc
     2941 gcaaaaatcg ttcaccgccg cattggtgat tttgcacgat catggggctt tggaatagat
     3001 gtcgcagcgc aataccgtaa aggcgattgg cgctttggtg cagtggccag agatattacc
     3061 accaccttca acgcctggag ttattcgctc agcgatgaaa tgatcaccac tttcctgcag
     3121 acaggtaatg aaattcctac gaactccatt gagcttacat tgccgaaatt gttattgggg
     3181 gcaggacgaa tgttccgtat caaagaaaat ttttctgtac tgggtgagtt tggtttcgat
     3241 tgcagtttcg atggaaaacg caataccctg atcaaatcta atttcatcag tatcgatccg
     3301 catttcggat tcgaagcagg atacaaaaac atcgtatttc tccgtggtgg aatggggaat
     3361 gtacagcgca gcatgcgaat cgatcaaagc ggaaatgaaa ccacttttca gcccaatatc
     3421 ggtataggtg ttcggataaa acgtttcgct atcgattatg cgcttaccga tatcggcgat
     3481 caatctgtag cacaatactc caacgttttt tcattgcggt ttgatattca caaagccgga
     3541 tcatgaaaaa aattctcctg ctgctactga tggtatggag cggattgtcc gtcttgggtc
     3601 agtcggcagg taacgaatgg atacagtttg gcggacccgg taagtatttt gttattcccg
     3661 ttacacaaag cggaattcat cgtatcgact acaacacact tcagaatgca ggattgccgc
     3721 tgggtacgat cgatccgcga aattttcagg ttttcggaag ggaaaaagag caattcattt
     3781 ttgttgaggg cgagtcggac ggaagttttg atccgggtga ttttattgaa ttctacgccg
     3841 agaagaatga tggttccgtc gattcactgc tctatgtagg aggtcgatca tcaatgccgg
     3901 atacgtatta cagtttgtac aacgatacca tctgttatta cctcacctgg aattcttcat
     3961 tggcgaacag acgtatgcct caattgaatt ctgctgcgta tgcttctttt acaccatccg
     4021 attggttctg gtcggaggtt tacaaaaaag tggcgctgcg gtattatcag ggtccgcaaa
     4081 tagttggcgc atcgtccagt cattttttat cgggcgaggg ctggtacaat gatcttttgt
     4141 atgcttcatc cggaagttct cagatcgatc ttttgccgac atcctttcct tatcagggag
     4201 cgggtgcacc ggatgcattt gcgaaatttg ttttttccgg caattcgaac gcaacaacat
     4261 ttccacctct gcaacccaat caccatatgc gggtcggatt tggtcctgcc aataccttgt
     4321 tcctcgatac cacttatgtc ggatatcgga tgatccgctc agacggtgtg tttcccaatg
     4381 ctttgctttc cggtgccgga tcgcgcattc aatttcaaac tgtaaacgat ctcggtgttg
     4441 cgtcggatat acaacaggtc gcattttcat cgatcaggta tccgcgtatt ccggatttaa
     4501 atacaggaac agcgcttgaa ttttttgttc ctgatcatgg atcgcaaaac catacccgta
     4561 tcgattttca gaatcctcca acggggaatt cttttgtgta tgtgttgggt gatacactaa
     4621 aacgtattgc tcccgaagtg aatggtgcat tttcctccgt tttagttccc aatcattttt
     4681 caggtacaga acaacgcttg tggttttctg cagaatcagc tgcaatttcc gtcgcacaaa
     4741 tttttccggt gaacggaaca ggcgatttta cggattatgc gcaactgaat cccgattcgg
     4801 tgtttttgat cattacgcat tcctcactga tgggagctgc acaacaatat gcactttacc
     4861 gatcttcacc tgcaggtggt tcttaccaga ctctggttgt ggacgtggat gaattgtatc
     4921 accaatacgg ttgtggtatt ccgaaacata ttttaggtgt gcgccgattt ttggcagata
     4981 tcaccaacga atggtccacc gctccttcgc atttgtttct gatcggtaaa ggaattcgtg
     5041 aagcaacgga atcttccaat ggaacatttc ccggatcgcg gcaatcactt tcggcgtatc
     5101 agcagaacct ggttccttct tatggttatc cttcttccga tcaactgatc tctgccggat
     5161 tgaatggtgt cttcaccgct cttccttcaa ttccaaccgg acgactatct gcttccgatc
     5221 cgcaaacggt gctgaattac ttaagtaaaa tgcagcaata cgaagcggca caatcacctt
     5281 tttcttcgta cacgattccc gataaagaat ggcaaaaaca aatcatgcat ttcggcggag
     5341 gaacaacaac atcggaacaa gctgattttt cctattgcct ttcggtatat gaagcattac
     5401 tcgaaggcgt gaaattcggc gggaatgttc agacctttct gaaacaatct tcagatccta
     5461 tcgatccgat cgaatacgct gaagtgatgc aacaaattca ggacggtgtt tccatcatga
     5521 cattctttgg tcactccacc ataaacggat ttgatcagaa cattgatgaa ccctctaact
     5581 ggaacaatac cggtaaatat ccatttctca ttggtaacgc atgttattca ggcgatattt
     5641 ttcaaccggg tattatcagt gcttccgaag aatttgtgtt tgaacaccaa aagggaacca
     5701 ttgcttttct atcgacttca aaactcggat tcgttcctaa tcttcattcc ttctcaaccg
     5761 ttttttttac aagagttgcc aatgtggatt acggattatc ggttggatac attctgcggc
     5821 agactattgg tcagattcaa ccctcaactg ttggcgcaaa cttattacag gaaaatgtgt
     5881 gtacgcaaat gacactgcat ggcgatcctg ccttaaagat caattatcat caggcaccgg
     5941 agctggtggt tcgtcagcag gatgtttttt tcaatccttc aacagtaacc ctcgccgatg
     6001 attcgatcaa tgtaaatgtg gtggtgacca acatcggacg cgcaacaacg gatactgtaa
     6061 tcgttgaatt gcgacggcat ttccccaatg gatccgattc catttatttc aaaacgctga
     6121 atggttcatt ctttaaagac acgcttactt tcagaattcc tgtccttcat aatattgcag
     6181 ttggcatcaa tcagttcgaa gtcctggtgg atattccgag ttttgtaacg gaacatgctg
     6241 acgaaacggg aaacaatcag gtgatggttc ccttgttcat cagttcgaac ggaatcattc
     6301 cggtttggcc atatgaatat gcaattgttc cggatagtgt aatggattta aaagcatcta
     6361 cctacaatcc actgcttggt acacgaacct atcgtttcga aattgataca accgatcagt
     6421 tcaacagtcc tgtacgaagg catcagctgg taacagctcc cggaggtgtg atcattgcgc
     6481 gtgcaacaaa ctggttgaat tccatgtcgg gcattgcttc gctattggag tttgccgaca
     6541 gtactgttta tttctggcgt gttactccgg atagttccgt ttattcctgg catgaaagtt
     6601 ctttccaata catctccgga ttaaatggtt ggggtcaggc gcatttttat cagtttgaag
     6661 cgaatcatca tcaaacgatg aattacagtc gtgccagccg ctcctggtac tgggatccga
     6721 atctccgcag aattggttgt gatgtttatt ctttcgctgc taattcttct gaattttttg
     6781 gaacactttg gacactcgac ggaaatcagc aggattacag tggttgtggt gttgatcccg
     6841 caattcatgt tgccgtcatc gatcctgtta ccatggaacc atggggaact tacggttgcg
     6901 atccggtaac gggtggatca tgtccaacct gcatcatggt gaatcaggat cgtcagtacg
     6961 gaaatgttaa cgacggttgt gcctgcagaa atcgggtaga attttatttc tcctttagaa
     7021 atacgaatcc cgtagaaatg gattctctgc tcagtatggt gaataaccat attcccgacg
     7081 gacattatgt gttgttgtac acctggcgtt ttaacgatta cagtacgtgg acacccgctc
     7141 attatgcgat gatgagtgca ttgggagcag gtgacagtat ttatcccgga cgaccaaacg
     7201 aatcatggat cgtgcttgcg cgtaagggag atccctcgtt taccaaagtg gtaatgggac
     7261 aaacaggtcc cggatttgtt accatgaacg atacgattgt cggatttgat tttaccggtg
     7321 caatgacatc cgttcgtgta ggaccatctt ccaattgggg ttcattatac tggaaataca
     7381 aatcactcga aagcccatcg cgcgattctg cacgtatttc tgttattgga attacgtcgt
     7441 ccggaagcga aacactttta ttcgatactt tatttacgcc ttacgattcc attctcaact
     7501 taggaagtct ggtggatgca tccgtgtatc cttacctgcg attgcgcgga tggtttacgg
     7561 atacaacttt tttcactccc gcacaggtgc agcgctggca gttggtgtac actcctgttc
     7621 ccgaagctgc agtgaacggt gcggcaggat tttatttgac acccggtgca gataccattc
     7681 ccgaagggaa accgtttgag cttgccattg ccattgaaaa catcagcaac caaacaatgg
     7741 attcgctgct cgtgcattat tggatcgaag atgcaaacag aatcaggcat tatttgactt
     7801 acggtcgaca agattccctg cggccgggac agattttatt ggataccatt caaatcgata
     7861 cccgtggatt ttccggattg aatagcattt ggatggaagt aaatcctgtt cctctgttgt
     7921 cggtgaacaa tacttacgat cagctggaac aattgcatgt caacaatttt gcgcagattc
     7981 cttttttcgt gcaacgggat attacgaatc ctattctgga tgtgactttt gatggaatgc
     8041 atattctcaa cggagatatt gtgagtgcaa aaccatttat tcttgtttca ctaaaagatg
     8101 aaaatccctg gatggtgatg aatcaggaat cggatacttc gttttttgca gtttatctca
     8161 ccgatcctac cggtaatcag cgcagaattt atttcagaga aaatggtcag cctgttcttt
     8221 tgtggtatcc ttcttccggt gcgggaggaa aattcagaat tgaataccag ccacaattgt
     8281 ttctggatgg aaaatacacc ttgttgatcc agtcgagcga taaatccggc aacaattcgg
     8341 gtagcaacga ttaccgcatt cagttcgagg tggtgaaccg gcaaacgatc actgaagtat
     8401 tgaattatcc caatcccttt accacgcgca cgcattttgt gtttacactc acagggagtg
     8461 aattgcccga tcagatgaaa attcagatca tgaccatcag cggaaaggtc gttcgcgaaa
     8521 tcaccatgga tgaattaggt cccattcgta tcggaagaaa catcaccgaa ttttattggg
     8581 atggtaccga tcagtttggt gatcgtctcg caaatggagt ttatctttac agagttgtta
     8641 cccgataccg cggacaagat gtcgaaaagc gggagtcggg tgccgatgaa tattttacaa
     8701 aaggcattgg taaaatggta ctgatgcgct aaacaaaaat gtatgatccg aattgaatat
     8761 atccttttac aaaatgatgc agcctttccg gctgaacagg aattttctgc gctttttatg
     8821 cccgggttct ggaaagatga aaacaagatc agtgtagaca gcgatgcttc cgttcagttc
     8881 gatcaacccg gaaaatggaa ttacctggat atcgaattgc tggatgatga cagaatcacg
     8941 caggcaacat tcagaataga tgtattccgc gaaagtaatt atccccgttc tgctatgttt
     9001 accgatgcgg atattgaaag cctggtgcgc gaataccgtc agggccgctt taatggttcg
     9061 gcagaaataa aaaatattgc aggctggtgt ttcacttcat taatgctcga aacagaagat
     9121 ccacgcatgc gcaaggagtt tatccaatcg gttgatcgtt ttctcaaggc agcaggtgga
     9181 atcgtagtgt atcccgaatt gatgaatccc gaaacgtttc gcagttttca ttcgctcgtt
     9241 caggatgtac ccgagccgga aattgagccg gaaa
//
LOCUS       DAOMYG010000058         9255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2492_length_9255_cov_8.722609, whole genome shotgun sequence.
ACCESSION   DAOMYG010000058 DAOMYG010000000
VERSION     DAOMYG010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 9255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9255
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2492_length_9255_cov_8.722609"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PK610_03075"
     CDS             <1..244
                     /locus_tag="PK610_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRJ34936.1"
                     /translation="DEKSFSDFKKESLTVFCGDGALKAKSLFEKSNFIFLPEIHPKAE
                     FMVELAEEKFSNGQFEDTAYFEPFYLKEFHTPQPKS"
     gene            complement(229..588)
                     /locus_tag="PK610_03080"
     CDS             complement(229..588)
                     /locus_tag="PK610_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34937.1"
                     /translation="MSDLLYYSENSIPHYVIHTKSTAIYSLLFFPLLLIIFSLSLILS
                     RHKLRVLHYSAIIAAIVMEAWSYLMYESPRHISFDEVFPAWVIAAGFFILNALLIHRR
                     LYKSIPDNSGILDQDFG"
     gene            complement(701..1528)
                     /locus_tag="PK610_03085"
     CDS             complement(701..1528)
                     /locus_tag="PK610_03085"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ34938.1"
                     /translation="MKKVIGSLLAVSGLLSCGGNNTTVEKEVIVKTDTVVITEQAPRD
                     SFMVFGHIEFPSTDGLTISASSYEVIPSEDYIILCHQAGYSRGEYREIAKELNKMGYN
                     CLAIDQRSGDKCNDMINETASRAKKDKKSCEYADAEQDILSAIDFVHKKTNHPIILWG
                     SSYSASLALKIAKGNEKVSAVVAFSPGEYLKGTNLSESISGLDKPVFATGAKKEMKDI
                     DKVLSGVTSPTKQVFTPSEEGVHGSSALWPSTANNAEYWTALKAFLDKLSPAVAENK"
     gene            complement(1584..1817)
                     /gene="atpC"
                     /locus_tag="PK610_03090"
     CDS             complement(1584..1817)
                     /gene="atpC"
                     /locus_tag="PK610_03090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HRJ34939.1"
                     /translation="MKVDILSPDSNLYSGEASAVSLPGKDGSLGILENHAPLITTLKK
                     GVVKLSTNEGEKTFDVGGGVVEVNHNKVIILAE"
     gene            complement(1867..3378)
                     /gene="atpD"
                     /locus_tag="PK610_03095"
     CDS             complement(1867..3378)
                     /gene="atpD"
                     /locus_tag="PK610_03095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRJ34940.1"
                     /translation="MSNKVYGKVAQVIGPVVDISFDNGVKLPNILDAVEVTKDNGQVV
                     VLEVQQHIGEDTVRAISMDSTDGFRRGLSVLSTGAPITMPVGDAIKGRLFNVVGDAID
                     GIKQVSKANGYSIHRPAPRFEDLSTSTEVLFTGIKVIDLIEPYAKGGKIGLFGGAGVG
                     KTVLIQELINNIAKGHDGYSVFAGVGERTREGNDLLREMIEAGIVKYGDKFMDSLHHG
                     GWDLSGIDAEQMKESKATFVFGQMNEPPGARARVALSGLTMAEYFRDGDEQSGGRDIL
                     FFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLASEMGAMQERITSTKRGSITSVQAV
                     YVPADDLTDPAPATTFAHLDATTVLSRKIAELGIYPAVDPLDSTSRILTPEIVGSEHY
                     DCAQRVKEILQRYKELQDIIAILGMDELSDEDKKVVHRARRVQRFLSQPFHVAEQFTG
                     LKGVLVPIEETIKGFNMIMNGDVDEYPEAAFNLVGTIEEAIEKGKKMMADATK"
     gene            3532..4482
                     /locus_tag="PK610_03100"
     CDS             3532..4482
                     /locus_tag="PK610_03100"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRJ34941.1"
                     /translation="MGPFTVKVYTSPESFKDVRNPVLTTGTFDGVHKGHRHIIDRLRS
                     IADEADGETVVFTFFPHPRMILFPDDTNLRLLNTQNEKIELLEKAGVDHLIIFPFSKE
                     FSRLNALEFVRDILVNQLHISRLVIGYDHQFGRNREGTIAHLRELSPMFGFQVEEIPA
                     QMVDSVNVSSTKIRAALTEGDIATAANFLGYNYPLTGLVVGGQQIGRSIGFPTANLKI
                     IDNHKLIPGDGVYAVNVSMKAGQFGGMLNIGSRPTINSGASQKSIEVNIFDFEGELYN
                     ELLKVEFIKKIRDERKFEDLSGLKLQLENDRLKAKEILFS"
     gene            4482..5054
                     /locus_tag="PK610_03105"
     CDS             4482..5054
                     /locus_tag="PK610_03105"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HRJ34942.1"
                     /translation="MRIILCILFFTPYLLPAQQDTMKTYTSLESALKNPEQVYRLKLQ
                     KQELKDFPKEIFLMKNLRQLDLSKNKIKVIPEEIGQLTQLEVLDISRNKIIDIPSTIG
                     QLINLRALILNQNFIESIPPEIGQLVKLVYLDMWSNNLWNFPEELTQLSNLKEFDLRN
                     IQLSFKEQEFIKKLLPNTKIHFSPACNCGK"
     gene            complement(5260..5895)
                     /locus_tag="PK610_03110"
     CDS             complement(5260..5895)
                     /locus_tag="PK610_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34943.1"
                     /translation="MKTIDRLSTSMGVRGNAPNKELALCCLKDPLLLQNLRPALLIYK
                     KGITADAAEVFTEVALQKPELVSPFFNDLFPLLKCKDNRARWEAMHAISAITFLNVEL
                     MRPLLPELERLVQEDKSIIVRDYVIEAIGNYSGVSLLCAKEALPYLEKFLSLENGRFA
                     GKCIFAMMQCAVFLPANAQLEKIANNYSLHEKASVQKAARKLLRILTQNNV"
     gene            complement(5895..7052)
                     /locus_tag="PK610_03115"
     CDS             complement(5895..7052)
                     /locus_tag="PK610_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ34944.1"
                     /translation="MINIISGISVAGIALAVTAMIIILSAINGLEGLVDTLSSKFDSD
                     IRITPLEGKSFDRNVISIAEIRSVEGVEAASEVVEELCVLKYGDNFVHGLLKGVQPEF
                     LQVSNVRENMIDGDTLLQMEDFYFCMVDVNIAATLELYVSDRPGEFQSVTIFAPMRNK
                     KMRVNRDPFNRHTLFLSGVFESSSDDELKPILVHRDLAASLLEYGESVSAYELRLKKG
                     SDVKQVKRSLQNKMGDAFKIQTRYEQNEILHKVSESEKFFVMIMLSFVLLLTSFNILA
                     SLTMLVIDKKKDIDVLISMGADKKMIRTIFFSEGLMINFFGGFIGLFIGASLVLAQQY
                     LHLIPLEGAVIDHYPVALKAFDFIWVAGIVGIVGIICSYVPVRFLVKRYFR"
     gene            complement(7192..8028)
                     /locus_tag="PK610_03120"
     CDS             complement(7192..8028)
                     /locus_tag="PK610_03120"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ34945.1"
                     /translation="MDYDPVKDSLGSFFNRSSTLRKVFYRLLDIVLLRSWHIQREIRK
                     WRRSAPQNAHILDAGSGFGQYSWYFSRLSKEWTILGVDVKRDYIADCNDFFRDQGRSN
                     VYFKVADLTRLNDEGAYDLILSVDVMEHIEEDVLVFRNFFKALRSGGVLLISTPSDKG
                     GSDALNNHGKSFIGEHVRDGYNMQEIKEKLKSAGFCKVKAHYSYGIPGRLAWKFAMKW
                     PMKMLGWSKGLFIILPVYYIVFLIPILILHYLDTFSPHRSGTGLIVRAEKNGNTREAQ
                     CK"
     gene            complement(8039..8377)
                     /gene="rbfA"
                     /locus_tag="PK610_03125"
     CDS             complement(8039..8377)
                     /gene="rbfA"
                     /locus_tag="PK610_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRJ34946.1"
                     /translation="MSTVRQNKVAALLQKELGNFFQRESAVYFGGSMISVTVVRVSPD
                     FSVAKVYLSIYAGPPREEVFQFIVSQTREIRYKLGQQIGKQMRIVPELIFYLDDSINY
                     AEEINQLLKK"
     gene            complement(8432..9160)
                     /locus_tag="PK610_03130"
     CDS             complement(8432..9160)
                     /locus_tag="PK610_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HRJ34947.1"
                     /translation="MMQSSVHTIPIYILHVRQGYEDRERFMKQQLDSFAMPFNWILEH
                     DLPDLKEEQIRRMFTGEMYGIRPEVSCAMKHIEAWKRIATSDEKGGLILEDDMLLNKE
                     FPVWMNKFLSEMDAKNQFSDPMLVSLENSTLMFVPRSERKEGQFLYKADRLRCAGAYY
                     LTREGAGFLLNELQKGPVEKPIDWWMGERKDLIIYWSHPTIAEQGSHNGMFASSLDHK
                     PKGIIRRIGFTVQRWVKSLKADRS"
BASE COUNT         2770 a         2058 c         1805 g         2622 t
ORIGIN      
        1 ggatgaaaaa tccttctcgg atttcaaaaa ggaatcgctt accgtttttt gtggcgacgg
       61 agcactaaaa gcaaagtctt tatttgagaa aagcaatttc atttttttgc ccgagattca
      121 tcccaaagct gaattcatgg ttgaactagc ggaagaaaaa ttttctaacg ggcaatttga
      181 agacaccgct tattttgagc ctttttacct gaaagagttt cacactcctc agccaaaatc
      241 ctgatcaagg atccccgaat tatccggtat acttttgtac agcctccgat gaataagtag
      301 agcgttcaga ataaaaaagc ctgcagcaat tacccaggca ggaaatactt catcaaaact
      361 tatatgtcgc ggcgattcat acattaaata cgaccatgcc tccataacga tcgctgcaat
      421 gattgccgaa taatgcaata cacgtagttt atgccttgaa agaatcaggg acaaactaaa
      481 aatgatgagc aacaaaggga aaaacaataa ggaataaatt gcagtacttt ttgtatgtat
      541 gacataatgc ggaattgaat tttccgaata atacaaaagg tctgacactc tagataagcg
      601 aatccaaaat ccggattgga ttgaaaaaaa tgcgagtccg atacacaacg gcaggacaat
      661 ccaaaacaaa ggaaaatatt tcaactattt atttagttga ttacttgttt tcagcgacag
      721 caggactcaa tttgtccaaa aatgctttta atgccgtcca atattctgcg ttattagcag
      781 tacttggcca caatgctgaa gaaccgtgaa cgccttcttc actgggagta aatacctgct
      841 tggtgggaga tgtaacaccg gaaagcacct tgtcgatgtc cttcatttcc tttttcgctc
      901 ctgttgcaaa aacaggttta tcaagcccgc taatgctttc gctaagattg gtacccttaa
      961 ggtattctcc gggagaaaat gcgacaacag cgcttacctt ttcgttgccc ttggcgattt
     1021 taagtgcaag cgaagcggaa taggatgagc cccaaagaat gatcggatga ttggttttct
     1081 tatgaacgaa atcgattgct gaaagaatgt cttgttctgc atcggcatac tcgcagcttt
     1141 tcttatcctt ctttgcgcga ctcgctgttt cgttaatcat gtcgttgcat ttatccccag
     1201 aacgctgatc gattgcaagg caattgtatc ccattttatt cagctctttt gcaatttcac
     1261 ggtactcacc acgactatat ccggcctgat gacaaaggat gatgtaatct tcagaaggaa
     1321 taacttcgta actgctagcc gaaatggtta gtccatcggt tgatgggaat tcaatatgac
     1381 caaaaaccat aaaggaatcg cgtggagctt gttcagtgat tacaacggtg tccgtcttaa
     1441 caatcacttc tttctcaacc gtagtattgt ttcctccgca ggaaaggagt cctgatacag
     1501 caagaagaga tccgattact tttttcataa tgctttcgtt ttctttaaac ataaaaaacc
     1561 ccgaaccgaa gtccggggtt gtattattct gcaagtataa taaccttgtt atggttcacc
     1621 tcaactactc ctccgccaac atcaaaggtt ttttctcctt cgttggttga aagcttaacc
     1681 actccttttt tcaatgtggt aatgagcggt gcgtggtttt ccagaattcc taatgatccg
     1741 tctttacccg gtaaacttac tgcagaagct tcaccggaat aaagattgct atcgggagaa
     1801 agaatatcaa ctttcataat tcattattta caacagttcg gctaagcgag ctttcgtcgg
     1861 tctgtattat ttcgtggcgt ctgccatcat tttctttcct ttttcgatcg cttcttcaat
     1921 cgttccaaca aggttgaatg ctgcttcagg atattcatcc acatcaccat tcatgatcat
     1981 attgaaacct ttgatcgttt cttcgatagg aacaagtaca cctttcaatc ccgtgaactg
     2041 ttctgcaacg tggaaaggct gagaaaggaa acgctgtacg cgacgtgcac ggtgaaccac
     2101 tttcttgtct tcatcagaaa gttcatccat accaagaatc gcgataatat cctgaagttc
     2161 cttgtaacgc tgaagaatct ccttaacacg ctgagcgcaa tcgtaatgtt cggaaccaac
     2221 gatttcagga gtaagaattc gtgaagtaga atccagtgga tctactgcag gatagattcc
     2281 caactcagca atcttacgac tcaatactgt tgtagcatca aggtgagcaa aagttgtagc
     2341 cggagcaggg tcggtcaagt catctgcagg cacatatacc gcctgtacag aagtaatgga
     2401 tccgcgcttg gtagaagtaa tacgttcctg cattgctccc atctcagaag caagagttgg
     2461 ctgataacca acagcagaag gcatacgacc aagaagtgca gatacctcag aacctgcctg
     2521 ggtaaagcgg aagatattat caatgaagaa aaggatatcg cgaccaccgg attgttcatc
     2581 accatcacgg aaatattcag ccatagtaag acctgaaagt gcaacccgcg cacgtgctcc
     2641 gggaggctcg ttcatttgtc cgaatacgaa ggtagccttc gactctttca tttgctcagc
     2701 atcgataccg ctcaggtccc atccaccgtg gtgaagtgaa tccatgaatt tatcgccata
     2761 cttcacaatt cctgcttcga tcatctcgcg cagaaggtca tttccttcac gggtacgctc
     2821 acctacccca gcaaatacag agtaaccgtc gtgacctttt gcaatgttat taatcaattc
     2881 ctggataagt acggttttac ctacaccggc accaccaaac agaccgattt ttcctccttt
     2941 tgcataaggt tcgatcaggt caattacctt gataccggta aaaagtactt cagtagaagt
     3001 agaaagatct tcaaaacgcg gtgctggacg gtgaatagaa tatccatttg ccttgcttac
     3061 ctgcttaatt ccatcaatcg catctccaac aacattaaac agacgaccct taatagcatc
     3121 tccaacaggc attgtaatag gtgctcctgt agatagcacg gatagaccac gacggaaacc
     3181 atcagtagag tccatagaga ttgcacgaac ggtatcttca ccgatgtgct gttgaacttc
     3241 aaggacaact acctgtccat tatctttagt gacttcaacg gcatcgagaa tgtttggtag
     3301 ttttactcca ttatcaaagg agatatcgac taccggtccg attacctgtg caacttttcc
     3361 gtagaccttg tttgacattg tattgaggat taaatgattg ttcctttttc gggcgcaaag
     3421 gtaggagaat tccccatttc caaaaggttc ctgttagtaa atttattttc ctaattttgc
     3481 gtcccgtcat gattcagatt cgcctttgtt ttcacggcgt ttatcggtcc attgggtcca
     3541 ttcacagtga aagtctatac cagcccagaa tcctttaaag atgttcgtaa tccggtactt
     3601 actaccggaa cctttgacgg tgtgcataaa ggccaccgtc atattatcga tcggctacgg
     3661 tcaattgcgg atgaagcaga tggagaaact gtggtattta cttttttccc gcatccccgc
     3721 atgatcttat ttcccgatga tacaaatctg cggttactga atacccagaa tgaaaagatt
     3781 gaacttctgg aaaaagctgg tgttgatcat ctcataattt tcccttttag taaagagttt
     3841 tcacgactga acgcacttga atttgtaaga gatatacttg tcaatcaact acatatttcg
     3901 cgtctggtga tcggatacga ccatcaattc ggaagaaaca gagagggtac gattgctcat
     3961 ctgcgggagc ttagcccaat gtttgggttt caggttgaag aaattcctgc tcaaatggta
     4021 gacagtgtga atgtttcgtc tacaaaaata cgagctgcct taacggaagg tgacatcgcc
     4081 actgcggcta attttctggg ttataattat cccctcacag gcctggttgt cggcgggcaa
     4141 cagattggga gaagtattgg ttttcctacg gcaaatctta agatcattga taaccacaag
     4201 cttattcccg gagatggtgt ttatgccgta aacgtgagca tgaaagccgg acaatttgga
     4261 ggaatgttaa atattggcag ccgaccaacg atcaacagcg gagcaagtca gaaaagcatt
     4321 gaagtaaata tatttgattt tgaaggcgag ttatacaatg aactccttaa agttgagttt
     4381 attaaaaaga tcagagatga gcgaaagttt gaagatctat ccggattgaa acttcagctc
     4441 gagaatgatc gtctaaaggc taaagaaata cttttcagct gatgcgcatt atcctttgta
     4501 ttcttttctt cacaccctat ctcctgccgg cacagcagga cacaatgaag acctatacca
     4561 gcctcgaatc tgcgctaaag aatcctgaac aggtttacag actcaaacta caaaagcagg
     4621 agctgaaaga ttttccgaaa gaaatatttc tgatgaaaaa tcttcgtcag ttggatctaa
     4681 gcaaaaataa aatcaaggtc attcctgaag agattggtca gctcacccaa ttggaagtgc
     4741 tcgacatttc ccgcaacaaa ataatcgaca taccctcaac gatcgggcag ctgatcaact
     4801 tacgcgcatt aattctgaat cagaatttca ttgaaagtat tcctcctgaa attggccaac
     4861 tggtgaaact tgtttacctc gatatgtgga gcaataatct ttggaatttt ccggaggaac
     4921 tgacgcaatt gagtaatctg aaagaatttg atttacgcaa tattcaattg agttttaagg
     4981 agcaggaatt cattaaaaaa ctgcttccca acaccaagat tcatttctct ccggcctgca
     5041 attgcggcaa ataatcaggg acaagcaccg gtgtgcacaa ctgttattcc gttcgaattt
     5101 gcgatgcatt cgttgcaata cgtcttccca tcacaaccac aaacagaagg acaatcttgc
     5161 gggcaaatcc cactatgatt tcgtttcatg gcaccatcgt agcaatcctg ctttgaacaa
     5221 gaaagcagca atagaaaaaa tataatccgg attccgattt cagacattgt tttgggttag
     5281 gatgcgtaat agttttcttg cggctttttg aacggatgct ttttcatgca aagaataatt
     5341 atttgctatt ttttccaatt gagcatttgc gggaagaaat actgcacatt gcatcattgc
     5401 aaaaatacat tttccagcaa atcgcccatt ttcaaggctc aggaattttt ccagatatgg
     5461 aagtgcttcc tttgcacaca aaagggaaac tccactgtaa tttccaatag cttcaatgac
     5521 ataatccctc acgataatgc ttttgtcttc ttgaaccaat cgttcaagtt caggcagtag
     5581 cggccgcatt aattccacat ttaagaaagt gattgcagaa atggcatgca ttgcttccca
     5641 gcgagcacga ttgtccttac attttaagag tggaaacaaa tcattaaaaa agggagaaac
     5701 taattcaggc ttttgcaatg ctacttccgt aaatacttct gcagcatcag ctgttattcc
     5761 ttttttgtaa atcagaagtg ccggacgaag attttgcaat aacaagggat cttttaaaca
     5821 acacaaagca agttccttat tcggggcatt cccacgaacc cccattgaag ttgaaagtcg
     5881 atcaatggtt ttcatcaccg aaaatatcgt ttaaccaaaa accgaacagg tacataagag
     5941 cagattattc ccacaatacc tacaattccc gctacccaaa taaaatcgaa tgcttttaat
     6001 gcaaccgggt aatgatcgat tacagctccc tcgagaggaa tcaaatgaag gtattgttgc
     6061 gccaaaacca aagatgctcc aataaacaaa ccaataaatc cgccaaaaaa attgatcatc
     6121 aagccctctg aaaaaaagat ggtgcgaatc atttttttat ctgcacccat actgatcaac
     6181 acgtcgatat cttttttctt gtcgatcaca agcatcgtta aagaggcaag aatgttaaag
     6241 ctggtgagca acaagacaaa agaaagcatg atcatcacaa aaaatttctc gctctcactg
     6301 actttatgta aaatttcatt ttgctcgtaa cgtgtctgga ttttgaatgc atcacccatt
     6361 ttattctgaa gggatcgctt aacctgctta acatcagaac cctttttgag gcgcaactca
     6421 tacgcagaaa ccgattctcc atattccaaa agtgaagctg ccagatcgcg atgaaccaga
     6481 ataggcttta attcgtcatc agaagatgat tcaaaaacgc cggaaagaaa tagtgtatgc
     6541 cggttaaagg gatctctgtt taccctcatt tttttatttc tcatcggcgc aaaaatggtc
     6601 accgactgga attcacccgg acgatcagaa acgtataatt cgagcgttgc agcaatgttt
     6661 acatcgacca tgcagaaata aaaatcctcc atctgcaata gcgtatcgcc atcaatcatg
     6721 ttttcccgga cattgctcac ctgcaaaaac tcaggctgaa cgcctttaag caatccatgc
     6781 acgaaattat cgccatactt cagcacacac aattcttcca caacttcact tgccgcttca
     6841 acgccttcaa ccgatcgaat ttcagcaatg gaaataacat ttcgatcgaa tgattttcct
     6901 tcaagtggtg taattcggat atccgaatca aatttcgaag agagagtgtc gactagtcct
     6961 tcaagtccgt taatggctga aagaataatt atcatagcag taacggcaag tgctattccc
     7021 gcaacggaaa tcccggaaat gatgttgatc acattggtgg atttcttcga aaagagatac
     7081 cggcgagcta tgaaaaatgg agtgcgcaga ttttttttct gcaatttagg aaatattcag
     7141 cggcaggatg tacccggtat aaaatgatcg gttattgcca gtgtctatta ttcatttgca
     7201 ctgtgcttcc ctggtatttc cattcttttc tgcccggaca attaaaccgg taccgctacg
     7261 gtgtggacta aacgtatcga gataatgaag gataagaatg ggaatcagga acacgatgta
     7321 ataaaccggg agaataatga ataagccctt cgaccatccc aacattttca ttggccactt
     7381 cattgcaaat ttccaggcca atcgtccggg gatgccatac gaataatggg cttttacctt
     7441 gcaaaaacct gcggatttca atttttcctt gatctcctgc atgttgtatc cgtcgcgaac
     7501 atgttccccg ataaatgatt ttccatggtt attcaaagca tcagatcccc ctttgtcact
     7561 tggtgtgctg atgagtaata cgcctccgga tcgaagtgct ttaaaaaaat tgcgaaatac
     7621 caatacatcc tcttcgatat gttccatcac atcaaccgaa agaatcagat catacgcccc
     7681 ttcatcattc agtcgggtta agtctgcaac cttaaaataa acattgcttc gaccctgatc
     7741 ccgaaaaaaa tcattgcaat cggcaatata atcgcgcttc acatccactc caagaattgt
     7801 ccactcctta ctcaatcggg aaaaatacca actgtattgt ccaaagccac ttcctgcatc
     7861 aagaatatgt gcattctgcg gtgctgatct gcgccatttg cgaatttccc tctgtatatg
     7921 ccaggaacgt aacaaaacaa tatccaagag ccggtagaac acctttctta gtgtgctgct
     7981 gcggttaaag aaggagccta atgaatcttt tacgggatcg tagtccatcg gacagctgtc
     8041 acttttttaa aagctgattg atttcctcag cgtaattgat gctgtcgtcg aggtaaaaaa
     8101 tgagttcggg aacaattcgc atttgttttc caatttgctg tcccagtttg tagcgaattt
     8161 ctctggtttg cgaaacaata aactgaaata cctcttcccg aggcgggccg gcataaatac
     8221 tgaggtaaac tttagccact gaaaaatcag gagacacgcg tacaacagtt accgaaatca
     8281 ttgaaccacc aaaatagaca gcactttctc tttggaaaaa atttcccaac tccttttgaa
     8341 gaagtgcagc tactttgttc tgacgtacgg tactcatggg cggcaaagat atttaaaata
     8401 tatgcacaga tgagccgcta atatgcttgt tttaagaacg atctgcctta agcgacttaa
     8461 cccatcgctg aacagtaaat ccaatgcgac gaattatccc ttttggttta tggtcaagcg
     8521 aagaagcaaa cattccgtta tgacttccct gctcagcaat tgttgggtgt gaccaataga
     8581 taatcaagtc tttacgctca cccatccacc aatcaatggg tttttctacc gggcctttct
     8641 gtaactcatt caacaaaaat ccggcaccct cacgagtaag gtaataggcg cctgcgcaac
     8701 gtaagcgatc ggctttgtaa aggaattgac cttctttgcg ctctgaacgc ggaacaaaca
     8761 tcaaagtgga gttctccaaa gaaaccagca ttggatctga aaattgattc tttgcatcca
     8821 tttcggaaag gaacttattc atccaaacag gaaactcctt attaagcagc atatcgtctt
     8881 caagaatcaa tccacctttt tcatcagaag ttgctatcct tttccatgct tcgatgtgtt
     8941 tcattgcaca ggaaacctcc ggtcgaattc catacatttc tccggtaaac attctccgga
     9001 tttgttcttc tttcagatcc ggcaaatcat gttccagtat ccaattgaaa ggcatcgcaa
     9061 aactatccaa ttgctgtttc atgaaccgtt cgcggtcttc gtagccctgg cggacgtgga
     9121 ggatgtaaat tggaatagtg tgaacagaag actgcatcat tttgcaaatc taaagctaaa
     9181 tcttgtccat ctaaaacatt gcctgcattt tcaaaagaaa tcataatgat ggcttagttt
     9241 atttatattg cgttg
//
LOCUS       DAOMYG010000059         9221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2503_length_9221_cov_8.535130, whole genome shotgun sequence.
ACCESSION   DAOMYG010000059 DAOMYG010000000
VERSION     DAOMYG010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 9221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9221
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2503_length_9221_cov_8.535130"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..1104)
                     /locus_tag="PK610_03135"
     CDS             complement(25..1104)
                     /locus_tag="PK610_03135"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRJ34948.1"
                     /translation="MNIRQHITTLLFRHDCVIVPGLGGFVANHHPSRVQDDLELVFPP
                     SKVIIFNKHLLTNDGLLAETIAREEELSFSDASEAIRIFTLELRDRMNKGERIPFEGL
                     GLLFLDEENSIQFQPDYSRNFLVEAFGLHPVVAREIVKEKTTSENDQFKEAIVIPIEK
                     AEEIHVRERSAMRRYWPVAAAMIPLIFYSIWIPTKTEFLATGRIEFADLNPFRTNEIV
                     YQARNNQPVFSIEKPVDEWAEIIANAGGPIARLPLNDHGSYLNISVTIDNPEAESTFV
                     SKRNASKRSEYHHHIIGGCFRELGNAEKLVSDLKAKGHDAYILDEKGGLHRVAINSFQ
                     SKSEATSALSKIRSEGINTAWLLEK"
     gene            complement(1123..1875)
                     /gene="kdsB"
                     /locus_tag="PK610_03140"
     CDS             complement(1123..1875)
                     /gene="kdsB"
                     /locus_tag="PK610_03140"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRJ34949.1"
                     /translation="MKVIGIIPARYASTRFPGKPLVDIEGTSMIMRVYQQASKTSLLN
                     EVIVATDDKRIFDHVLQHGGKAIMTSVDHHSGTDRCLEALQKTGKSFDIVVNIQGDEP
                     FIAPEQIEELIHCFDDAAAEIATLTRRIEDPNMLNDPNKVKAVKSNKDFALYFSRQCI
                     PFQKNIPESDWLKHQSYFLHVGLYGYRSHILHEISQLPVSPLEKSESLEQLRWLENGY
                     KIKIAETKHSSYGVDSPEDLKSLLITFGKKGS"
     gene            complement(1878..2852)
                     /locus_tag="PK610_03145"
     CDS             complement(1878..2852)
                     /locus_tag="PK610_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRJ34950.1"
                     /translation="MRNIPEYMVIVEKGLADLHFPDRPKELYDPLRYILTLGGKRMRP
                     VLLLMAHELFGGKAENALPQAIAIELFHNFSLIHDDIMDNAPLRRGKKTVHEKWNSNV
                     GILSGDAMLVIAYQQMSKCAADKLPEVVRVFNRTAIEVCEGQQYDMEFETRDDVSIEE
                     YINMIALKTSVLLGGALEIGAILANANKKDAEHLNEFGKNLGIAFQLQDDLLDAYGDP
                     DKFGKKVGGDIVANKKTYLVLKALKDADANQNKELQQLIQSGTIPEETKIRQVLELFN
                     ALSIPEKTRMAMDEYYAKSIHHLNAISLDASKKAPLEKLAESLMVREH"
     gene            complement(2853..3713)
                     /gene="rfbA"
                     /locus_tag="PK610_03150"
     CDS             complement(2853..3713)
                     /gene="rfbA"
                     /locus_tag="PK610_03150"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HRJ34951.1"
                     /translation="MKGIILAGGSGTRLHPLTLAVSKQLMPVYDKPMIYYPLSTLMSS
                     GIRDILIISTPHDLPHFKKLLGDGKDLGCNFTYEEQTVPNGLAQAFVIGASFVGKDSV
                     ALILGDNIFYGTGLGRNLRSHYDPKGAVIFAYHVSDPERYGVVEFDADMKAISIEEKP
                     KQPKSNYAVPGLYFYDNQVIDIAKNLKPSARGEYEITDVNKEYLGRGELQVSILDRGT
                     AWLDTGTFASLMQAGQFVQVIEERQGMRIGCIEEVAYTMGYINKEQLAKLADPLTKSG
                     YGTYLLNLIK"
     gene            complement(3735..6347)
                     /locus_tag="PK610_03155"
     CDS             complement(3735..6347)
                     /locus_tag="PK610_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRJ34952.1"
                     /translation="MLSVLALITAFWLYMAITGIRINHKFQTMLPEKDTANIVFQDMK
                     KRFGEDGMVMVIGINDKDLYTPEKFRNWKKLGADLRTIDGVDSVFSEADMYGLYKNDS
                     LKKFEILPLCREIPDTPEELDSTRKLIHSFPFYNGILYNDVTGAHLMMVFINPNKFNS
                     KERGTTIPEVVAIGEKYESVFGSIHFSGLPFIRETLFNTLKSELRFFIGFSAAVTCLI
                     LFLFFRSIRVIIACMIVIIVGVIWAFGTMGLLGYEISQLMALIPPLMIVIAIPNCIYL
                     ITKYHQEFMRAGNKYRALNMVIRKIGGATLMTNATTALGFSTFIITENDKLREFGMVS
                     AINVISLFFLSIIIIPIVFSYMRNPSVKQTKHLEKRWVEHSIDFLVNLVSRRRWLVYL
                     STIAVIIVGIIGVSLVRTTGAITEDLPDSSRVKKDLVFIEENFGGMVPFEIIIDFKSK
                     GQLKNPNHAIRNLQKVDSIQRVLDNEDYFSKSLAVTDALKFVNQAFNGGEMESYRLPG
                     KRDFIYLKPYFERLKENKNGPGLKGFIDSTETQSRITMQVKDLGAKQLVAIEYRLEKK
                     IDGILNPDRREVDSSFNAAFATKEIERDSSLKLFYENYPRIYNLLVEELADGDSVILA
                     QLEENPDKVYARHSDVDYKEKLQRAINNSRADVIFTGTAVAFAKGTKYLIGNLLSSLV
                     FAIVSISALMALLFRSLRMVMISMLPNLVPLLITGAIMGLFDIPLKVSTVLVFSIALG
                     ISVDDAIHFLAKYRQELKTGKSIKESAIHSISEAGVSMMYTSIVLFCGFLIFTFSEFG
                     GTKALGLLISITLMVAMLCNLVILPSLLMSLDKWVTTQAFREPYLEIFDEEIDEDLSS
                     LRIERKDDQEEQFL"
     gene            complement(6440..8866)
                     /locus_tag="PK610_03160"
     CDS             complement(6440..8866)
                     /locus_tag="PK610_03160"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRJ34953.1"
                     /translation="MRTVFILCQLILVLPLYAQYRISGTVLDMATREPLAFVNIISAD
                     GKFGASTDIEGRFTIQSSEKIELLRVSYIGYYSREYLVDHSLTRQVIYLEKGNTELET
                     VEVLPGINPADTLMELVYANRKRNDPEKSVHFSYTSYNKLIVTLAIDSALFLHPEKIA
                     QLDTNKKQVIELFEKQHLFMAESVTERQFRLPDNNKETVIASRISGFQNPVFNLLATE
                     LQSFTFYREYISLGGIQYEGPVAPNASKRYFFLIEDTLFTEKDTLWRISFRPKKGKSF
                     NGMKGVLLISSNGFALVQVIAEPANKKSQDLGIKIMQQYEFAASRQWFPVQLNTTLFF
                     PNVEIENMELVGYANAYLTNIKIDPEQKKKNSPAYIALETDPDAGKREENFWNGHRTD
                     SLTEKERTTYHVIDSLGKAQKLERRFNAMMYLLDGQVPIKKINLDLTRLISFNDFEGF
                     RAGLGLRTNDRISNYFSIGGYFAYGFKDKDWKYGADLLLKIPGKDVHLNLNWQHDLEE
                     TGGVTFFERRGMNLSQDLSELYRNRFDFSDKYEAVFGCRLNKHIKVFPYGNVQYRTSF
                     PGYGQQISMSNGIDLFRDEYILAETGLGLRIQLRERLIRSGNRIISEGSDFPLVWIKI
                     SHGTDQLASGELNYNKVDLRIEKRHRIRRMGHISYRLQAGMMDGQQPPLGILYNPRGT
                     WRPIRELNVVSGYGFEVMRTNEFLADKYAALHFRYEMGSLFFKGKFKPQFILASNHFS
                     GSLNQQKLLLPDVKVPTGIYSESGLLVNSIIRSGISGIGIGFFYRYGNHAFESTIDNM
                     AIKFSFSFVM"
     gene            8885..>9221
                     /locus_tag="PK610_03165"
     CDS             8885..>9221
                     /locus_tag="PK610_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRJ34954.1"
                     /translation="MRLPIFGEVIFYSDTARLFKTFSFRKAWNLLRLYRSFRASRRSK
                     SAVHKGMPVAISIEPTTACNLGCPECPSGLKVFSRPTGNLRLDLNKRILDELAPWLWY
                     VNFYFQGEPY"
BASE COUNT         2645 a         1964 c         1829 g         2783 t
ORIGIN      
        1 ggttgggcat tttttaatga agtattattt ttcaagcagc caggctgtat taattccctc
       61 gcttcttatt ttgctcagag cggaagttgc ttccgatttg ctttggaaac tattgatagc
      121 aacgcgatgc aaaccacctt tttcatcgag gatataagca tcatgcccct ttgctttcag
      181 atcgctaaca agtttttctg cattgcccaa ttcacggaaa catccaccga taatatgatg
      241 atggtattcg ctgcgcttag acgcattccg tttacttaca aatgtacttt cagcttcagg
      301 gttgtcgatc gtgacagaaa tattcagata gctgccatga tcatttaaag gaagacgagc
      361 aatgggccca cctgcatttg caatgatctc tgcccattca tcgaccggtt tttcaataga
      421 gaaaacgggt tggttgttgc gtgcctgata tacaatttca ttggtacgga aaggattaag
      481 gtctgcaaat tcaattcttc ctgttgccag aaattcagtt tttgtgggta tccagatcga
      541 ataaaaaata agcggaatca ttgctgctgc aaccggccag taacggcgca tagcagaacg
      601 ttcgcgaaca tgaatttctt ctgctttttc aatcggaatg acaattgctt ctttgaactg
      661 atcattctcc gatgtggttt tttccttcac aatttcacgc gcaactaccg gatgaagtcc
      721 aaatgcctcc accaaaaaat tgcgggagta atcaggttga aactgtatac tgttttcttc
      781 atccaaaaaa agaagaccca aaccttcaaa cggaatgcgt tctcctttat tcattcgatc
      841 acgaagttct aaagtgaaaa ttcgaatggc ttcagacgca tcgctaaacg aaagttcctc
      901 ttctctggct attgtctcag caagaagtcc gtcgttggtg agtaaatgtt tattgaaaat
      961 aatgactttg gaaggcggaa aaaccaactc aagatcatct tgcacacgcg aaggatgatg
     1021 attggcaaca aagccaccaa gtccggggac gataacgcaa tcgtgccgga aaagcaatgt
     1081 ggtaatgtgt tgccgaatgt tcatggattg gtaaagatac gctcaggaac cttttttccc
     1141 aaaagtaatt aacaatgact tgagatcttc cggactatca actccatagc tgctatgttt
     1201 agtctcagca atcttgatct tgtatccgtt ttccagccag cgtaattgct ccagcgattc
     1261 cgatttttcg agcgggctaa ccggtagttg gctgatttcg tggagtatgt gtgagcgata
     1321 cccgtacaaa ccaacatgca ggaagtagga ttggtgcttc agccagtcgg attcgggaat
     1381 gtttttttgg aagggaatac attgcctgga aaagtacaag gcaaagtctt tattgctctt
     1441 cactgcttta actttgttag gatcattcag catgttcggg tcttcaatac gtcgggtaag
     1501 cgttgcaatt tccgctgcag catcatcgaa acagtggatg agttcttcaa tttgttccgg
     1561 agcaatgaaa ggctcatcgc cttgtatgtt gaccacaatg tcaaatgatt tgcctgtttt
     1621 ttgcaatgct tcaaggcaac gatctgttcc gctatgatga tccacgcttg tcattattgc
     1681 tttaccacca tgctgcagca catggtcaaa aattcgctta tcgtcggtgg caactattac
     1741 ttcgttaaga agcgatgttt tagatgcttg ttggtagaca cgcataatca tggatgtccc
     1801 ttcaatatct acaagcggtt taccggggaa tcgggttgag gcgtatcgtg caggaatgat
     1861 tccgattact ttcatgctca atgctctctt accattaaac tttctgcaag tttttcaagt
     1921 ggtgctttct tcgaagcatc aagtgaaatg gcatttagat ggtggatgga ttttgcataa
     1981 tattcatcca tggccattcg ggttttttca ggaatagaca gagcattgaa aagctccagt
     2041 acttgtcgga ttttcgtttc ttccggtatc gttccggatt gaatcaattg ctgcaattcc
     2101 ttattttgat ttgcatcagc atcttttaat gcttttaaaa cgagataagt ttttttattg
     2161 gcaacaatat ctccgccgac tttctttccg aatttatcag gatccccata agcatccagc
     2221 agatcatcct gtaattgaaa tgcgataccc aaattttttc caaattcatt caaatgctca
     2281 gcatcttttt tattggcatt ggcaaggatg gcgccaattt caagcgctcc accaagaagc
     2341 acggaagttt ttaaagcgat catgttgatg tattcctcaa tactcacatc atcacgtgtt
     2401 tcaaattcca tgtcgtattg ttgtccttcg cagacttcaa tagccgtacg attgaatacg
     2461 cgaactactt cgggaagttt gtcagctgca catttcgaca tttgctggta ggcgattacc
     2521 agcattgcat ctcccgatag aattcccacg ttgctgttcc atttttcatg aacggttttt
     2581 tttcctcttc gcaacggagc attgtccata atgtcgtcgt gaattaagga aaaattatga
     2641 aataattcaa tggcgattgc ttgtggtaat gcattttcag cttttccccc gaataattca
     2701 tgggccatta gcaataatac gggtcgcatt ctttttccgc ccaatgtaag gatgtagcga
     2761 agtggatcat acagctcttt gggtcgatca ggaaaatgga gatcagcaag acctttctcc
     2821 actattacca tgtattctgg aatatttcgc atttatttaa tcagattcag caaatacgtt
     2881 ccataaccgc tttttgtaag tgggtctgcc agtttcgcca gttgttcttt gttaatgtaa
     2941 cccattgtat aggcaacttc ttcgatgcaa ccaatacgca taccttgtcg ctcctcaata
     3001 acctgcacaa actgcccggc ttgcattagt gaagcgaagg ttccggtatc cagccaggct
     3061 gttccacgat caagaatgga aacctgcaac tctcctcgac ccagatattc tttatttaca
     3121 tcagtaattt catattcacc tcgtgcactt ggcttcaggt ttttggcaat atcaattacc
     3181 tgattgtcgt aaaaatacaa accgggtacc gcataattcg atttgggttg ttttggtttt
     3241 tcctcaatgc tgattgcttt catatcagca tcaaactcaa ccacaccata tcgttcggga
     3301 tcactaacat gatatgcgaa aatgactgct cctttaggat cgtaatgcga tcgtaaattt
     3361 cttcccagac ctgtaccgta aaaaatatta tcgccaagaa taagcgcaac actatcctta
     3421 ccaacaaaac tagctccaat tacaaatgct tgtgcaagtc cattgggaac agtttgttct
     3481 tcgtaggtga aattgcaccc aaggtctttt ccatcaccca atagtttttt aaaatgcgga
     3541 agatcatgtg gagtagaaat tatcaggata tcgcggatac ctgaactcat taatgtagac
     3601 aaaggataat aaatcatcgg cttatcgtac accggcatca attgcttact taccgcaagt
     3661 gttaatgggt gaagacgcgt tccggatcca ccggccagaa taataccttt catgataatt
     3721 caattaacat ttagtcagag aaattgttcc tcctgatcat cttttctttc gattcgcaaa
     3781 ctgctgagat cctcgtcaat ttcttcatca aaaatttcca gataaggttc acggaaagct
     3841 tgtgttgtta cccatttgtc gaggctcatt aatagcgatg gtaaaatcac aagattacat
     3901 aacatcgcaa ccatgagcgt aatggagatc aataagccca atgcttttgt tcccccgaat
     3961 tcagaaaagg tgaaaatgag aaatccgcag aacaacacaa tcgaggtata catcatgctg
     4021 actcctgctt cagagatgga atgaatggca gattccttga tcgattttcc ggtttttaat
     4081 tcctgccggt atttagcaag aaagtgaatg gcatcgtcca cagaaattcc aagagcaata
     4141 gaaaaaacaa gaaccgtaga aacttttaac gggatatcaa agagccccat aattgctccc
     4201 gtaatcagca gaggaaccag attgggaagc atcgaaatca taaccatccg cagcgatcgg
     4261 aataacagtg ccattaatgc cgaaatagaa acaatcgcaa aaacaagact agataataaa
     4321 tttccgatta gatattttgt tcctttcgca aacgcaactg cagtgcctgt aaaaatcaca
     4381 tctgctcttg aattatttat ggcgcgctga agtttttcct tgtaatcgac gtcggagtga
     4441 cgcgcataaa ctttatcagg attttcttct aattgtgcaa gaataacact gtctccatct
     4501 gccagttctt caacaagcaa attgtaaatt ctggggtagt tttcataaaa caatttcaga
     4561 ctgctgtcgc gttcaatttc ttttgtcgca aaggctgcat tgaaggatga gtcaacttct
     4621 cttcgatccg gattgaggat gccgtcgatc tttttttcaa ggcggtattc aatcgctaca
     4681 agttgtttcg ctccaagatc tttcacttgc attgtaattc ggctttgtgt ttctgtgctg
     4741 tcaatgaaac cttttaagcc tggtccgttt ttattttctt ttagtcgttc aaaatagggt
     4801 ttcaggtaaa tgaaatcacg ctttcctggc aggcgatacg attccatttc tccaccgtta
     4861 aaggcctgat taacgaattt taatgcatcc gtaaccgcca gtgattttga aaaatagtcc
     4921 tcattatcta atacacgctg aatggaatct actttttgca aattgcggat ggcatggttt
     4981 ggatttttta actgcccctt actcttgaaa tcaataatga tttcgaatgg aaccattcca
     5041 ccgaaatttt cctcaataaa aaccaaatcc ttctttacac gtgaactgtc aggaagatct
     5101 tcagtgatcg ctcctgttgt gcgcacaagc gaaacaccaa ttataccaac aatgattaca
     5161 gcaatggtac tgagatatac cagccaacgg cgacgggaaa caagattgac caggaaatcg
     5221 atagagtgtt ctacccatct tttctcaagg tgtttggtct gtttcactga tgggttccgc
     5281 atatagctga acacaatcgg aatgatgatg atggatagga aaaacaatga aataacattg
     5341 attgcagaaa ccattccgaa ctcccttagc ttgtcgtttt ccgtaataat gaaagtggaa
     5401 aacccaagtg ctgtggttgc attggtcatt aatgttgcgc cgccaatttt acgaatgacc
     5461 atattcaatg cgcggtattt atttccggca cgcatgaatt cctgatggta ttttgtaatc
     5521 agataaatac aattgggtat tgcaataaca atcatcagcg gcggaatgag cgccatgagt
     5581 tgtgatatct cataacccaa cagtcccatt gttccaaatg cccatataac accaacaatt
     5641 attacgatca tacaggcaat aattacccgt atggaacgga aaaacaggaa gagaatcaag
     5701 caggttactg cagcagaaaa cccaatgaag aaacgcaatt cggattttag tgtgttaaat
     5761 aatgtttccc gaataaaagg caatcccgaa aaatgaatac tgccaaatac cgattcgtat
     5821 ttctctccaa ttgcaacaac ttcaggtatg gtggttccac gttctttgga attgaactta
     5881 tttggattaa tgaacaccat catcaggtgc gcgccggtta catcgttgta aaggattccg
     5941 ttgtaaaagg gaaaagagtg aatgagtttg cgggtactgt ctagttcttc aggagtatcc
     6001 gggatctccc tgcaaagcgg taaaatttcg aattttttaa gggagtcgtt tttgtataaa
     6061 ccatacatat ctgcttccga aaaaacagaa tcaacgccat caatggtacg taaatcagcg
     6121 ccaagttttt tccagtttct gaatttctct ggagtgtaaa gatctttgtc gttaatacca
     6181 atcaccatca ccatgccgtc ttcaccaaat cgttttttca tatcctgaaa aacaatattg
     6241 gccgtgtcct tttcaggaag catagtttga aacttatgat tgatgcggat tcctgttatt
     6301 gccatataca gccaaaacgc agtaatcaag gcaagtacag aaagcatctg tatgcggtat
     6361 cgaagaattt tagatgctat ccaggcccac atatggtaga gttgttgtca aaagtaggaa
     6421 aatgatgcga accaaatggt tacataacaa aggagaaact gaacttaatg gccatattgt
     6481 ctattgtcga ctcaaaagca tgattgccat agcggtagaa aaacccaata cctatgcccg
     6541 aaatacctga acggataata ctattcacca ataaaccgga ttcggagtat attccggttg
     6601 gaacttttac atcgggcaaa agcaactttt gctgattcag gcttccgcta aaatggttac
     6661 tggcaagaat aaattgcggc ttgaatttac ccttaaaaaa caaacttccc atttcataac
     6721 ggaaatgcaa tgcggcatat ttgtctgcca ggaattcatt ggtccgcatg acttcaaagc
     6781 catatcctga aacaacgttt aattcccgaa tgggtctcca tgtgccccga ggattgtata
     6841 aaattccgag gggcggttgc tgcccatcca tcattcccgc ctgtaaacgg tacgaaatat
     6901 gtcccattcg tcggatgcga tggcgctttt caatgcggag atctactttg ttgtaattca
     6961 gttctccact tgccagctgg tctgtaccgt gactgatttt aatccaaacg agagggaaat
     7021 cggaaccttc ggaaatgatt ctgttcccgc tcctgatcaa acgttcccgt aactgaatac
     7081 gaagccctaa gcctgtttct gcaagaatat attcgtcacg aaaaagatcg ataccattgc
     7141 tcatggagat ttgctgacca taacccggga aactcgttct gtattgaaca ttaccatagg
     7201 ggaacacttt gatgtgtttg ttcaaacggc agccaaaaac agcttcgtat ttatccgaaa
     7261 aatcaaaacg attacgatag agttcgctga ggtcctgact taagttcata ccccttcttt
     7321 cgaaaaacgt cactcccccg gtttcttcca ggtcgtgttg ccagtttaga ttgagatgca
     7381 catccttccc cggtattttc agtaacagat ctgcaccgta tttccagtct ttgtctttaa
     7441 atccataagc gaagtagcct cctatcgaaa aataatttga aatccggtcg tttgtccgca
     7501 atcccaatcc tgcacgaaaa ccttcaaagt cattaaagct gataagtcgt gtaagatcaa
     7561 ggttgatttt ttttatggga acctgaccat ccagtaaata catcatcgca ttgaaccgcc
     7621 gctctagctt ttgcgctttt cctaacgaat caattacatg ataagtggtc cgctcttttt
     7681 cagtcaaact gtctgttcga tgaccattcc agaaattttc ttccctttta cctgcatctg
     7741 gatcagtttc cagagcgata taggccggac tatttttctt tttctgttct ggatcaattt
     7801 tgatgttagt taaataggca ttggcataac caaccaattc catgttttct atttccacat
     7861 tcgggaaaaa caatgttgta ttaagttgca cgggaaacca ttggcgcgaa gcagcaaact
     7921 catattgctg catgattttt atacccagat cctgactttt tttattggct ggttctgcaa
     7981 ttacctgaac aagggcaaac ccattggaac tgataagcaa aacacctttc attccgttaa
     8041 aggattttcc tttttttgga cgaaaagaaa tacgccacag agtatccttt tcagtaaaca
     8101 gagtgtcttc tataagaaaa aagtagcgtt tacttgcgtt tggtgcaaca ggaccctcat
     8161 attgaattcc tccgagggaa atgtattctc tgtaaaaggt aaaactttgc aattccgttg
     8221 ccagcagatt gaacaccggg ttttgaaatc cggaaatgcg cgatgcaatc acggtttctt
     8281 tattgttgtc gggcaaacgg aattgtcgct cagttaccga ttctgccata aataaatgtt
     8341 gtttctcaaa aagctcaata acttgctttt tattggtatc aagctgtgcg attttctccg
     8401 gatgtagaaa gagtgcggaa tcaatggcaa gggttacaat caatttgttg taggaggtat
     8461 aagaaaaatg aactgatttt tcaggatcgt tccttttacg gttcgcataa acaagttcca
     8521 tcaaggtatc tgcaggattt attcccggca aaacctcaac ggtttccagc tcggtatttc
     8581 ctttttccag ataaatgact tgtctggtga gagaatgatc tacaaggtat tctctcgaat
     8641 aatagccaat ataagaaaca cgtaaaagct caattttttc agaagattga atggtaaacc
     8701 ggccttcgat atcggttgaa gcaccaaatt tcccatcagc gctgatgata ttaacaaaag
     8761 caagaggctc gcgggtggcc atatcaagta ctgtaccgct aatcctgtat tgagcataca
     8821 gcggcaaaac cagaataagt tggcaaagaa tgaaaactgt gcgcatggtt caaatataca
     8881 ttaagtgcga ttgcctattt ttggagaggt gattttttat tcggatacgg cacggctttt
     8941 caaaactttt tcattccgca aggcttggaa cttacttcgt ttgtaccgat cgttccgggc
     9001 atcacgcaga tctaaaagtg cagtccataa gggaatgcct gttgcaattt ctatagagcc
     9061 tacaacagca tgcaatcttg gttgtcctga atgcccaagc ggactaaaag tattttcacg
     9121 accaacagga aatcttcgtt tggatttaaa caaacgaatt ctcgatgaac ttgctccatg
     9181 gctatggtat gtcaactttt atttccaggg agagccctat a
//
LOCUS       DAOMYG010000060         9108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2541_length_9108_cov_9.389926, whole genome shotgun sequence.
ACCESSION   DAOMYG010000060 DAOMYG010000000
VERSION     DAOMYG010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 9108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9108
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2541_length_9108_cov_9.389926"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PK610_03170"
     CDS             <1..679
                     /locus_tag="PK610_03170"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRJ34955.1"
                     /translation="SVSQVFASNMEARHRRAVQHLLDKALQEKELLIQEMNHRVKNNF
                     GILISLIRLSKQAGKTKDPLVLLDEYEHRIMSMLRMHDLLSQSQYINKVDLSSYLKKL
                     AEEFKVSAENTMASVKFVSKGEVVMVNSGMALHIGLITTEIYLNSLKHGLPKQQHFVF
                     EIVMDVQPSSIIIKAGDDLKGFDYSTANRNGSLGLQLIADIASDLGAKMITPSGEDSF
                     YHFELPQ"
     gene            complement(729..2360)
                     /gene="groL"
                     /locus_tag="PK610_03175"
     CDS             complement(729..2360)
                     /gene="groL"
                     /locus_tag="PK610_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007743854.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRJ34956.1"
                     /translation="MAKDISFNIESREALKKGVDTLANAVKVTLGPKGRNVVIEKKFG
                     APHVTKDGVSVAKEIELSDPIANMGAQMLKEVASKTADVAGDGTTTATVLAQAIVTAG
                     LKNVAAGANPMDLKRGIEKAVKVVVEELKKMSQTVGDDNKKIEQVATISANGDSAIGK
                     LIAEAMAKVGKEGVITVEEAKGTETEVKTVEGMQFDRGYLSPYFVTNTEKMITDLDKP
                     YILITDKKVSSMKDILPVLEQVAQQGKQLLIVAEDVDGEALATLVVNKLRGALKIAAV
                     KAPGFGDRRKAMLEDIAILTGGTVISEEQGYRLDAADITHLGSCEKITIDKDNTTIIN
                     GKGSAEAIQQRVSQIKAQMESTSSDYDKEKLQERLAKLSGGVAVLYIGAATEVEMKEK
                     KDRVDDALHATRAAVEEGIVPGGGTAYIRCINGLDNAKPENDDEKTGIDIIRKSLESP
                     LRQIVENGGLEGSVIVNKVRDGKDDFGYNASTETYENLFKAGVIDPTKVSRVALENAA
                     SIAAMILTTECVLSEKKEKKAAAPAMPGGGMDDMY"
     gene            complement(2421..2720)
                     /locus_tag="PK610_03180"
     CDS             complement(2421..2720)
                     /locus_tag="PK610_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRJ34957.1"
                     /translation="MATATLEKVEVKVKPLADRVLIEPVQAESKTAGGIIIPDTAKEK
                     PQRGRVVAVGNGKKDEPMSVKVGDTVLYGKYSGTEISIDGTDYMIMKESDIYAII"
     gene            complement(2854..4446)
                     /locus_tag="PK610_03185"
     CDS             complement(2854..4446)
                     /locus_tag="PK610_03185"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ34958.1"
                     /translation="MRRILLFSLLMNLVFITSSKATHITGGDFSVKWLNGNNFQVTLK
                     LFRDCASGGAAFDSPINIRVFDNVTNAVVSNFSMTLLSVTPITLGDECYSPPSSICIE
                     EGFYQTTISLANNPNGYYMAWERCCRNPGIVNVNNPQNAGMVFYVQVPDPAIQNSNPE
                     FGSYPPEGYLCIGTTNYIDFSVTEPDGDSLAFELEDLYMGSLSTNLNPAPNSANPKPY
                     AICTWQAPFGLGNIVGGTPPMTINPQTGIITCNPTTTGIFAFAVMIKEYRNGVKISET
                     RREIQYYVLNCVFDDLPAIMLPDTVNIQVLTSGCFDVVVIDEDVTDTVSIFLSSNTFG
                     QGAALVTPDPYVTTPTPLYYYYFTNSSNIPDSILLPAPDLVMGSYVGIGGVGLRYCFP
                     TDCPDLEITGMNLDVAAFSLGCSGDTNFINRVVQIKVIPPQGIEEIVPNVFTPNGDGL
                     NDVFRLEGTPNPCWDSIHVEIYNRWGQLIYVSDEVEFKWDGKTKAGKEVPEGTYFVIL
                     KGTFGDTDVTRQYPVTLLRTKK"
     gene            complement(4509..5393)
                     /locus_tag="PK610_03190"
     CDS             complement(4509..5393)
                     /locus_tag="PK610_03190"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRJ34959.1"
                     /translation="MNQPQNILFIINPHSGIGKQKLMEEYIGKNLDRRLFTPTICYTE
                     RKGHAYDLAAEAQKNGIPIIAIAGGDGTVNEAGRALMGTNSSLLIIPTGSGNGIANHY
                     KIPDNAEAILRSLEHWKTIKTDVGMINEFPFFGFCGFGFDAHIAHEFAKLKSRGMAGY
                     TRLVFREFKKFIPQEIELRCNGEVIRENPFILSVANVSEYGNGVKIDPDADTCDGELG
                     VCSIRKVNPFQLAVLFFKSRKGNISHSEFYRKRKGDSFEIISSQALKAHIDGEPWDFT
                     DKHLKIRITDKQLQLLVP"
     gene            complement(5436..6857)
                     /locus_tag="PK610_03195"
     CDS             complement(5436..6857)
                     /locus_tag="PK610_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRJ34960.1"
                     /translation="MLNRRLTYIFIFGLISSMAVISGCRKDRLIDNPSAKLAFSQDTV
                     IFDTLFTTVGSTTRQLKIYNRNRGKVNIDRIYLAGNAGNSYRMNVDGLPGTSFSDIEI
                     DGGDSLYIFVEVTLNPNSLTTPLLVTDSIIFITNGNVQDVDLVAWGQDAYFHTPPNGA
                     TSPFFSLPCNSIWTNDKPHVIYGYPVVDSTCSLLIQPGTKIYSHPNSALVVYNGGTLN
                     ADGDPGNRIIFQGDRLEPNYDSLPGQWSGIILLEAGNSRIEHCDIRNASVGVRVQTIS
                     GTLSPLVMEYVRIENMSNFGVWNYVAGDISMTNCMINNCGQYAFICTGGGFVRINHCT
                     FANYWNYTDRRVPAFGMANFFEDATGALVLVDLNAEVSNSIIYGNEGNEFAVNMNAGA
                     VGNFVFNTCYIKSDQDLSNTTNFINISKNVDPRFEEPNEQRYKLQSISPCINTGNLIF
                     RIDYDLDFFTRDALPDIGCFEFQ"
     gene            6983..7237
                     /locus_tag="PK610_03200"
     CDS             6983..7237
                     /locus_tag="PK610_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002662609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HRJ34961.1"
                     /translation="MKQGIHPENYRLVVFKDMSNDYMFLCRSCADTKDTITYSDGNEY
                     PLVKLDISHMSHPFFTGKVKLVDTAGRIDKFKSRYDKFKK"
     gene            7354..8508
                     /locus_tag="PK610_03205"
     CDS             7354..8508
                     /locus_tag="PK610_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HRJ34962.1"
                     /translation="MKICLTDEQEWNRLLPLTYTRSVADIRVGILTIREKWEMILGEK
                     VYVEAPDYLAGRYEGVPKGEKVIRVNSAIIPDEHFVERVRVLQPGQAIEGNHGLMAWV
                     DGNDDVLLSAPFSGEIRLRHPWDIFMLNGLALERDFELITAGRTSQAISATNTVIGKG
                     KIFLEEGAKVEACIINAHEGPVYIGKNAEVMEGSVIRGGLALCEGAVLKMSAKIYGAT
                     TIGPYCKVGGEINNSVFFGYSNKAHDGFLGNSVIGEWCNLGADTNNSNLKNNYGTVKV
                     FSISENAFISTGIQFCGLIMGDHSKSGINTMFNTGTVVGVNANVFGGGFPPKHIPSFS
                     WGGADGFSTFDLDRSFDVSERVMSRRNLPFTDSDRDMMRQIFQLSSRFRG"
     gene            8590..>9108
                     /locus_tag="PK610_03210"
     CDS             8590..>9108
                     /locus_tag="PK610_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRJ34963.1"
                     /translation="MNENQFGDLFEFSGMIDGDTDLIPLITPEDEEQMNREATPDDLP
                     ILPIRNNVLFPGVVIPITVGRDKSIRLINDAYKAKTVIGVIAQKKQDVEAPGFEDLFR
                     FGTVAQVLRVLKMPDGSTTVIIQGKKRFEMLDLITEEPYIRANVRQYMDLPHGMKDDK
                     LSALVSSLKDLSL"
BASE COUNT         2467 a         1978 c         1984 g         2679 t
ORIGIN      
        1 gagcgtttct caggtttttg catcaaacat ggaagcccgc catcgacgtg ctgttcagca
       61 tttactggat aaagcattgc aggaaaagga attgctcatt caggaaatga accaccgggt
      121 gaaaaacaat ttcggaattt tgattagttt gatacgatta agtaaacaag ccggaaaaac
      181 aaaggatccg ctggtactac tggatgaata tgaacaccgt atcatgtcca tgctgcgcat
      241 gcacgatttg ttgtcgcaat cgcagtacat taataaagtt gacctatcat cctatctgaa
      301 aaaacttgcc gaagaattta aggtttcagc tgaaaacaca atggcttcgg tgaagtttgt
      361 ttccaagggt gaggttgtaa tggtcaattc agggatggcg cttcacatcg gattgataac
      421 gactgaaatt tatctcaatt cactcaaaca tggtctgcct aaacaacagc attttgtttt
      481 tgagatcgtg atggatgttc agccttcgtc catcatcatc aaagcaggag atgatctgaa
      541 aggatttgat tattcaacag caaatcgcaa cgggagtctc gggctccaac tcattgctga
      601 tatcgcttct gatctgggtg cgaaaatgat cactccttcc ggagaggata gcttctacca
      661 cttcgaatta ccgcaataaa aaatcccccg gcaatattga cgggggattt tgattttcgt
      721 tttggagatc aatacatgtc atccattcca ccgcccggca ttgcaggagc agcagctttt
      781 ttctcttttt tctcgctgag tacacattcg gttgtcagga tcatcgcagc aattgatgca
      841 gcattttcga gagcaacacg actcactttg gttggatcga tcactcctgc tttaaacaga
      901 ttttcgtaag tctctgttga agcattgtat ccaaaatcgt ctttcccgtc gcgaaccttg
      961 ttaacaataa ccgatccttc cagaccgccg ttttctacga tttgacgcag tggtgattcg
     1021 agtgatttgc ggatgatatc aattccggtt ttttcatcgt cgttttcagg cttagcattg
     1081 tcgaggccat tgatacaacg aatataggct gtaccaccac cgggaacaat tccttcttct
     1141 actgcagcac gagtagcgtg aagggcatca tcaacacggt cttttttctc tttcatttca
     1201 acttcagtag cagctccgat ataaagaaca gcaacacctc cgcttaattt tgccaaacgc
     1261 tcctgtaatt tttctttatc ataatcggag gatgtgcttt ccatctgagc tttgatttgt
     1321 gaaacacgct gctgaatggc ttctgcactt ccctttccgt taataatggt tgtattgtcc
     1381 ttatctatgg taatcttctc acaacttccc aaatgggtaa tgtcggctgc atccaaacgg
     1441 tatccctgct cttcagaaat cacggttcct ccagttagaa tggcaatgtc ttcaagcatc
     1501 gctttacgac gatcaccaaa acctggagct ttaaccgctg caatttttaa tgcaccgcga
     1561 agtttgttta ccactagtgt tgccagtgct tcaccatcta catcttctgc tacaatcaat
     1621 aactgttttc cttgttgcgc tacttgctca agtacaggta aaatatcctt catgctgctt
     1681 acttttttgt cggtaatcag aatgtaaggt ttgtcaagat cagtaatcat cttctctgta
     1741 ttcgtcacaa aataaggcga caaataccct ctgtcgaatt gcattccttc taccgtttta
     1801 acttcggttt cagttccttt tgcctcttca actgtgatca ctccttcttt tccgactttc
     1861 gccattgctt ctgcaatcag ctttccaatt gcgctgtcgc cgttagctga aatggtagca
     1921 acctgttcaa ttttcttatt gtcgtcgcct acagtttgcg acattttctt cagctcttcc
     1981 actaccactt tcacggcttt ttcaattccg cgctttaggt ccattgggtt ggctccagct
     2041 gcaacattct ttaatcctgc agttacaatg gcctgggcaa gaactgtagc tgtagttgtt
     2101 ccgtcgccag caacatctgc agttttacta gcaacttctt tcagcatttg tgctcccata
     2161 ttggcaatgg gatctgataa ctcaatttct tttgcaacag atacaccgtc tttggtgaca
     2221 tgcggtgctc cgaatttttt ttcgataacc acattacgtc cctttggacc gagtgtaact
     2281 ttaactgcgt tagcaagggt gtcgactcct tttttaagtg cttcgcgcga ttcaatgttg
     2341 aaacttatgt ctttggccat ggttgcttag tttaagtatg attttaattt gtaatgtgtg
     2401 aagttgattt gaatagtcga tcagatgatt gcgtagatat cgctttcttt catgatcatg
     2461 taatctgtac cgtcaatgga gatctccgtg ccggaatatt ttccgtaaag cacggtgtca
     2521 cctactttta ccgacatcgg ttcgtctttt tttccgttac ccactgccac gactctgccg
     2581 cgttggggtt tttccttggc tgtatcggga atgatgattc cacccgcagt cttactttcc
     2641 gcctgaacag gttcgatcag cactcggtca gccaaaggtt taacttttac ttcgactttc
     2701 tctaaagttg cagttgccat aggtaaatgt ttttttggtt agtacacgca tcacgacaaa
     2761 gcacgtacca atggccggaa atggtgagaa atggaaaaaa atggcagggt taactgccaa
     2821 ttcgatcagc ggatgtccga aaaaatgtca ggcctacttc ttggttcgca aaagtgtgac
     2881 aggatactga cgcgtaacat ccgtgtcgcc aaaagttcct ttcagtatca caaaataagt
     2941 gccctccgga acttccttac cggcctttgt ctttccatcc catttgaatt caacttcatc
     3001 tgaaacatag atgagttgtc cccaacgatt gtagatttca acatgaatgg aatcccaaca
     3061 agggttcggt gttccttcca gacggaaaac atcatttagc ccatccccat tgggagtgaa
     3121 tacattcggt acaatttctt cgatgccttg aggtggaatt acttttatct gaacaacccg
     3181 gttgatgaaa ttggtatcac ccgaacaccc cagcgaaaag gctgccacat cgagattcat
     3241 tccggttatt tccaggtccg gacaatccgt cgggaagcaa tacctcaagc caacgccgcc
     3301 tataccaacg taagaaccca tcaccaaatc cggagccggt agaaggatgg agtcaggaat
     3361 atttgaagaa ttggtaaaat aataatagta gagcggagtg ggagtggtaa cataaggatc
     3421 gggagttacc aaagccgcac cctgaccgaa tgtgttggag gaaaggaaaa tggatacagt
     3481 atcggttacg tcttcgtcaa ttacaaccac atcaaaacat ccactggtta aaacctgaat
     3541 atttaccgta tcgggaagca tgattgcagg aagatcatcg aaaacacaat tgagaacgta
     3601 atactgaatt tcacgtcgcg tttctgaaat tttaacaccg ttgcggtatt ccttgatcat
     3661 tacggcaaat gcaaaaattc cggtcgttgt agggttacac gtaataattc ctgtttgcgg
     3721 attgatggtc attggaggag ttccgccaac tatatttccc aaaccgaaag gagcctgcca
     3781 ggtacaaata gcatagggtt ttggatttgc tgaattaggt gcaggattga ggttcgtact
     3841 cagacttccc atgtaaagat cttctaactc aaaagccagt gaatctccat caggttcggt
     3901 tacactaaaa tcgatgtaat tggttgttcc gatacagagg tatccttcgg gaggatagga
     3961 tccgaattcg ggattggaat tctgaatagc aggatcaggg acctgtacgt aaaataccat
     4021 tcccgcattc tgaggattgt taacgtttac aatacccgga tttcgacaac aacgttccca
     4081 ggccatgtaa taaccgttgg gattattggc aagactgatt gttgtctggt aaaaaccttc
     4141 ttcaatacaa atagaggaag gcggactata gcattcatca cccaacgtaa ttggagtaac
     4201 gcttaaaagt gtcattgaaa aattggaaac aactgcattg gttacattat cgaatacccg
     4261 gatattgatc ggactgtcaa atgcagctcc accacttgcg caatcgcgga atagtttgag
     4321 tgtaacctga aaattgttcc cattcagcca ttttacgctg aagtctcctc cggtaatgtg
     4381 ggtggccttg ctggatgtaa tgaacacaag gttcattaat aaagaaaaga gtaaaattct
     4441 cctcatgaaa gtgtctggtt ttattcgaga cggaagatac gcactttccg ttgcctgaca
     4501 taaataaatc agggaacgag caattgaagc tgtttatcgg tgattctgat tttcaggtgc
     4561 ttgtctgtaa aatcccaagg ctcgccgtcg atgtgtgctt ttagagcttg tgaacttatt
     4621 atctcgaagg aatccccttt gcgctttcgg taaaattctg aatggctgat atttcctttc
     4681 ctggatttaa aaaacaagac agcaagctga aagggattta cttttcggat actgcaaaca
     4741 ccaagttcac catcgcacgt atcggcatcg ggatcaattt ttaccccatt tccgtattcc
     4801 gatacatttg caaccgacag aataaacggg ttttccctta taacttcgcc attgcaccgg
     4861 agttcaattt cttgtggaat gaattttttg aattcccgga aaacaagtcg cgtataccca
     4921 gccatgcctc gtgatttcag tttcgcaaat tcatgcgcaa tatgtgcatc aaagccaaaa
     4981 ccgcaaaaac caaaaaaggg aaattcattg atcattccaa catcggtttt gatcgttttc
     5041 caatgctcca atgatcgcag gatcgcttct gcattatccg ggattttata atgatttgca
     5101 attccgtttc ctgatccggt ggggatgata agtaggctgc tgttggtccc catcaatgca
     5161 cggcctgctt cattaacggt accgtcgcct cctgcaatgg cgataatggg aattccattt
     5221 ttttgagctt cagccgcaag atcataggca tgtcccttgc gttcagtgta acagatggtg
     5281 ggtgtgaata gtcttcgatc caggttttta ccgatatact cttccatcaa cttttgcttc
     5341 ccgattccgg aatgaggatt gataatgaaa agtatgtttt gtggctgatt caactcctca
     5401 ataatttatc ccaagccaca tcctaaattc attgcttact gaaattcaaa acaacctata
     5461 tcagggagtg catcacgggt aaaaaagtca agatcgtaat cgattcggaa aatcagatta
     5521 ccggtattaa tacaggggga aatggactgc agcttgtacc gttgctcatt gggctcttca
     5581 aaacgcgggt cgacattctt gctgatattt ataaaatttg tggtgttgct caaatcctga
     5641 tccgatttga tataacaggt gttaaacaca aaatttccaa ctgctccggc attcatattc
     5701 actgcaaatt cattgccttc attcccgtaa ataatactgt tacttacttc agcattcaaa
     5761 tcgactaaca ccaatgctcc ggtagcatcc tcaaagaaat ttgccatccc aaatgcagga
     5821 acccttctgt cggtatagtt ccagtaattg gcaaaggtgc aatggttgat tcggacaaat
     5881 cctccgcctg tgcaaatgaa ggcatactga ccgcaattat tgatcatgca attggtcatg
     5941 ctgatgtctc cggcaacata attccatact ccgaaattcg acatgttttc aatgcgcaca
     6001 tattccatta ccaatggtga taaggtcccg ctgatggttt gcacccttac gccaaccgat
     6061 gcatttctga tatcacaatg ttcaatgcgg ctgtttccgg cttccaataa aatgatcccg
     6121 ctccattggc cgggcaaact gtcgtagtta ggttccagtc ggtcgccctg aaaaataatt
     6181 ctgtttcccg gatctccatc ggcatttaag gtgccaccat tgtaaaccac cagagcggaa
     6241 ttgggatgcg aatagatttt tgtcccgggc tgaatgagca gtgaacaggt tgaatcaaca
     6301 acggggtaac cgtaaataac atgtggcttg tcgttggtcc aaattgaatt acaaggcaag
     6361 gaaaaaaacg gactcgttgc gccatttggt ggggtgtgaa agtaggcatc ctgcccccag
     6421 gcgaccaaat ccacatcctg aacattgccg ttagtaatga atatgataga gtctgttaca
     6481 agcaacggag tggttaaact gttgggattg agggtgactt ctacaaaaat gtaaaggctg
     6541 tcaccaccat cgatttcaat atcgctaaag ctggttcccg gcagaccatc aacattcatc
     6601 cggtacgaat ttcctgcgtt tccggccagg tagatcctgt cgatattgac ctttccccgg
     6661 ttccggttat aaatcttcaa ttgtcgcgtt gtggatccta cagtggtaaa aagtgtgtcg
     6721 aaaatgaccg tgtcctgcga aaatgccagt ttggcggaag ggttgtcgat cagccggtct
     6781 ttgcggcatc cgctgattac tgccattgaa ctgatcagtc caaaaatgaa aatgtaggtc
     6841 aggcggcggt tcagcatggt gcaaaaatag ggattattgt acttacggac ttttttagcg
     6901 attattgctg aaacctttct atttcctttt tggttggaaa ggattttttt ctatttttgc
     6961 agcccgataa taagtaaacg tcatgaaaca aggcattcac cccgaaaatt accgcttggt
     7021 tgtgtttaaa gacatgtcca acgattatat gttcctttgt cgctcttgcg cagacactaa
     7081 agacactatt acgtattctg atggtaatga gtatcctttg gtgaagctcg atatctcgca
     7141 catgtcgcat cctttcttta ccggtaaggt gaagcttgtg gatacagcag gtcgtatcga
     7201 taaattcaag agccgttacg ataagttcaa gaagtaattc tgatatactg ttcagaaagc
     7261 cccgactgaa aagccggggc ttttttttgc gataatgagg tagaccattt acctgtacag
     7321 catgtatatt tgatacccaa acaaccaatt cttatgaaaa tctgcctgac cgacgaacaa
     7381 gaatggaacc gtttactgcc tctgacctac acgcgttcgg tggctgacat aagggttgga
     7441 attctcacca tccgcgaaaa atgggagatg attctgggtg aaaaagttta tgtggaagct
     7501 ccggattacc tcgcaggcag gtacgaagga gtacccaaag gcgaaaaggt cattcgggta
     7561 aacagtgcta ttattccgga tgaacatttt gtcgaacggg tgagggtttt gcagccgggt
     7621 caggcaattg aaggtaatca tgggttaatg gcatgggtgg atggcaatga tgatgttttg
     7681 ctgagtgccc cgttttcagg ggaaattcgt ttgcgtcacc cctgggatat ttttatgctt
     7741 aacggtttag cgcttgaacg tgattttgaa ttgatcactg ctggaagaac atcacaagct
     7801 atttcagcca caaataccgt gatcgggaag gggaaaatct ttctggagga aggtgcaaaa
     7861 gtggaggctt gtataataaa cgcacatgaa ggtcctgttt atattggcaa aaatgcagag
     7921 gtgatggagg gatctgtgat tcgtggggga ttggcattgt gtgagggtgc agttttgaaa
     7981 atgtcggcca aaatttatgg cgcaactacc atcggaccct attgtaaagt gggaggagag
     8041 atcaataatt ccgtcttttt tggttacagc aacaaagcac acgatggttt cctcggaaat
     8101 tctgtcattg gcgagtggtg caatctgggg gcggatacca ataattcgaa tctgaaaaac
     8161 aattacggaa cggtaaaagt gttcagtatt tcagagaatg cttttatcag cactggtatt
     8221 cagttttgcg gactcatcat gggagatcat tcaaaatcag gaatcaatac catgttcaat
     8281 actggaaccg tagttggggt aaatgcgaat gtttttggag gtggatttcc tcccaagcac
     8341 atcccttcct ttagctgggg tggtgcagat ggattttcca ctttcgacct cgaccgctca
     8401 ttcgatgttt cagaaagggt aatgtcgcgc agaaacctgc cgtttaccga cagcgatcgc
     8461 gacatgatgc gccaaatttt ccagttaagt tccagattca gaggataaga atctgctttt
     8521 cctgacgtta ttacatgcct gcagcaatgg cacgttcatt gtcgttgttg cttcacatat
     8581 cctacatcca tgaatgaaaa tcaatttggc gacttgttcg aatttagcgg aatgattgat
     8641 ggcgacactg atcttattcc actcatcaca cctgaagatg aagaacaaat gaatcgtgag
     8701 gcgactcccg atgatctgcc tattttacct atacgtaaca atgtcctttt tccgggggtg
     8761 gtaattccta tcacagttgg aagagataaa tcgatccgtt taattaatga tgcctacaaa
     8821 gcgaaaacgg taattggcgt aatcgcacag aagaaacaag atgtagaagc acccggattt
     8881 gaagatcttt tccgtttcgg aactgtggcg caggttttac gtgtgcttaa aatgcccgat
     8941 ggaagtacaa cagtaattat tcaggggaag aagcgctttg aaatgcttga tctcattacc
     9001 gaagaaccat acatccgcgc aaatgtgcgt caatacatgg atcttccgca tggaatgaaa
     9061 gacgataaac ttagcgcgct tgtgagttcg cttaaagatt tatcactg
//
LOCUS       DAOMYG010000061         9061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2555_length_9061_cov_10.247724, whole genome shotgun sequence.
ACCESSION   DAOMYG010000061 DAOMYG010000000
VERSION     DAOMYG010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 9061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9061
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2555_length_9061_cov_10.247724"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2313)
                     /locus_tag="PK610_03215"
     CDS             complement(<1..2313)
                     /locus_tag="PK610_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ34964.1"
                     /translation="MKNTLLFVLALFWMSTLQAQQTIRGQVIDEASKAPLPGVNIVLI
                     GTDPPVGTSTDGNGYFKLQNVPLGRQSLKFSFIGYEDRTLTDLIVTAGKEVVLTVSMT
                     ESVEMLETVDVVYDRSADKDQSVNEMTIVGARSFNLEETRKYAGSLGDPSRMAANFAG
                     VVAGNDSRNDIVVRGNSPSGMLWQMEGLNIPNPNHFGALNSTGGPVSMINNNNLDKSD
                     FLTSAFPAQYGNALAGVFDLHLRKGNTEKHEFLGQIGFNGFEFGAEGPMGKKKKSSYL
                     LNYRYSTLGVFQQMGINFGTGTATPLYQDINYKFYFPVGKNGQLTFFGINGNSSVNFL
                     GNDVDTTQIDLYGDENTNTRVRYATYINGLSYNHQLSSKTNLRLVAGHSRTYERFKGD
                     SISIITREEFPSGEARFDTDKFSAHFIVNHKLNARNTIVGGVMTDYVKFGLFNKVVLG
                     GVTDVVRVDSDDVYILGQTYVQWKHRFNKGFSLVGGIHSQLLNISNSISAEPRLALRW
                     NFRKGMALSAGYGYHAQMQSVYTYFVQTNTTDGAVQTNKSLGFSNSHHIVLTHDWNVT
                     SYLRIKTELYNQQLFNVPIERNASSFSALNSGADFAPSNNDSLVNEGTGRNYGLELTI
                     ERFFNKGYYFLITTSIFDSKYKGSDGVERNTAFNTGYVLNVLGGKEFKLNDKGNVLAL
                     NIKTSFVGGRYLTPLNDTLSRLYGVAVYDNERAFSEKQSDYFRTDIKISYRKNLKKST
                     MEFSIDLQNVSNHKNVFRQSFNPRTGMMVTQ"
     gene            complement(2435..3034)
                     /locus_tag="PK610_03220"
     CDS             complement(2435..3034)
                     /locus_tag="PK610_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRJ34965.1"
                     /translation="MDPINALKAYYQQLNPSMTEEDWHFFQSVQRVRRVKKGEMIVKP
                     GEVCNHVSFVAEGLVRYFYFNEGKEITADFCPENCYTSEYCSFLTRRPTMDYLEALED
                     SILVEMDYEGMQSLYSRGALFERMGRLIAEGLFISLSTRNSTLMTKSPEDRYNEFFEA
                     HPQMFQRVPLYMIASYLGITPETLSRIRKRLTEKAKQNS"
     gene            3140..5179
                     /locus_tag="PK610_03225"
     CDS             3140..5179
                     /locus_tag="PK610_03225"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRJ34966.1"
                     /translation="MATAPKTVKKSILSTADFKKLVLQGIPDRLPKAKTWDPEINHAP
                     KRKEILSAEEKKLALRNALRYFPKKHHAVLAKEFAEELKSYGRIYMHRFRPDYEIYAR
                     PIQHYPAKTKQAAAIMHMLSNNLDHAVAQHPHELITYGGNGAVFQNWAQYLLTMQYLS
                     TMTEEQTLVLYSGHPMGLFPSHKDAPRVVVTNGMMIPNYSKPDDWERFNALGVTQYGQ
                     MTAGSFMYIGPQGIVHGTTITVMNAARMKSKGKDYKGMLFVTAGLGGMSGAQPKAATI
                     AGVVSITAEVNPKATYKRHEQGWVDEVYADVDKAIDRAEEARSKKMALSIAYDGNIVD
                     LWERLAQRNVKVDLGSDQTSLHNPWAGGYYPAGISYEESNRMMAEEPEKFKEEVQKTL
                     RRHAAAINKLNAQGMYFFDYGNAFLLEASRAGGDVKGTDREFRYPSYVQDILGPMCFD
                     YGFGPFRWVCTSADPKDLEATDKIALDVMRKIAATAPMEIKQQMQDNIRWIEEAQKNK
                     LVVGSQARILYADSEGRIKIAEAFNKAIKAKKIKSPIVLGRDHHDVSGTDSPYRETSN
                     IYDGSRFTADMAVHNFVGDGFRGATWISLHNGGGVGWGEVINGGFGMVLDGSKDADRR
                     LKNMLFWDVNNGIARRAWARNKEAMFAIQREMKRSKKLKVTLPNLVDDKNLEGLV"
     gene            complement(5260..5628)
                     /locus_tag="PK610_03230"
     CDS             complement(5260..5628)
                     /locus_tag="PK610_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34967.1"
                     /translation="MKKLLSTLVFVLIATFGFSQNLTATGGSAALGKAKASTGVFEFT
                     ATGLDIASVNQNAKTYASWFTVAAKEGKGSISLSITMHKNESQTRRVMHRYFVTLNVQ
                     TVTVDSTEMKVEDFVEKYLI"
     gene            5710..6390
                     /gene="pnuC"
                     /locus_tag="PK610_03235"
     CDS             5710..6390
                     /gene="pnuC"
                     /locus_tag="PK610_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HRJ34968.1"
                     /translation="METKHGRVFLGMEWQEVFFSTWFEWMGYPVSGLEFIGSIAGLVG
                     VYLAAKSNILTWPVGIVNVVCFFFVFYTARLYSDMLLQFYYFSVSIIGWVNWNKSGEQ
                     KLKHEWIGQQWRFRLPLITIVATLGIGYLIEHLHILFPSVLHEPADLPYLDTLIGVLS
                     IQATFLQARKKIDCWVIWIVTDALCTGVYWKKGIHLVSLEFLLFTMLAIFGLMQWLQK
                     HREELAGK"
     gene            complement(6421..7782)
                     /locus_tag="PK610_03240"
     CDS             complement(6421..7782)
                     /locus_tag="PK610_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRJ34969.1"
                     /translation="MFSIIDIETTGLDPKRDRITEIAIVVHDGLTVVERYSTLIHPER
                     SIPQTISRITGITNEMVAEAPKFYEVAKKILELTRDKIFVAHNVEFDFRFIQEEFRSL
                     GYSYKRDKLCTVRLSKKLIPGRKSYALGRLCEALQIPLHNAHRALADADATASLFSYL
                     MQLKSAHPVYKLQNLEAINTGRTQKIQQYILKKLPEKTGVYRFLGKGENILYIGKSTN
                     MRQRAIQHYQSKEKKARKMIGEVYNVEFEETGSELIALLLESEEIKRHQPPYNRARRK
                     KAFAYALESAIHDNGVLQFQIVPTADSKEPLKIFHTWISARAFLNNWMDNEYVCPKFC
                     GAFDMEGPCFHFQIRKCNGICCGKEDPEIYNARTQNLIETCAGDKSDQLIKGAGRNSH
                     EYSFVLMLEGKYAGYGYLNVDVTFDSLQEYRKRVVPFDYFPDADELIRSFLRSGTVDV
                     EAR"
     gene            7960..8766
                     /locus_tag="PK610_03245"
     CDS             7960..8766
                     /locus_tag="PK610_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34970.1"
                     /translation="MNAAALPFGRTRQAQHQFLPEKKSNLRAVKSDDKEIMLQPFRDL
                     ASAIVEMGQFKLSGERELSEGNKSLLFLRNTREDLHIKGEPVKEYLLIVASHESSGKS
                     FLATYHIPENRGNRYCHKLQALFAQQLSPAELQQQALRTIRNYESGRRDLYQLLHAFS
                     TVRADEELVEEVRNEVVRYLQQTLRNKPTLSGKGIVTQINALDNVMSVCRHSRHHTLK
                     TLFDSITTFSTYHLDIREPAQAEGLGADLNQHIFHLLEREWQAELAEIHP"
     gene            complement(8797..>9061)
                     /locus_tag="PK610_03250"
     CDS             complement(8797..>9061)
                     /locus_tag="PK610_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34971.1"
                     /translation="TNANKAIGPVVIVSKGKRGRPRKDLAPNVQLLEKKVAKLEKRLG
                     KMTGVLQEIADNSLLRKVFGIGSRRPGVKKSTGKRGRPAKNIN"
BASE COUNT         2647 a         1979 c         1926 g         2509 t
ORIGIN      
        1 ttgtgtcacc atcataccgg tgcgtggatt gaaactctga cgaaaaacat ttttgtgatt
       61 gcttacattc tgaagatcga ttgaaaactc cattgtgctt ttcttcagat tcttcctata
      121 agaaatttta atgtcggtac ggaaataatc ggattgcttt tcagaaaatg cacgttcgtt
      181 atcgtaaact gcaactccat acaatcggct taatgtgtcg tttaacggtg ttaaatacct
      241 tcctccaaca aaagaagttt ttatgttgag cgcaagaaca ttacccttat cattcaactt
      301 aaactccttg ccacctaata catttaatac ataaccggta ttgaacgcag tgtttcgttc
      361 tacgccatcg ctgcctttgt attttgaatc gaaaatagag gtggtgatca aaaaataata
      421 ccccttattg aaaaagcgtt cgatcgttag ctcaagtccg taatttctgc cggtgccttc
      481 atttaccaat gaatcgttat tgctgggtgc aaaatcagca ccactattca gggcagaaaa
      541 agaagaagcg tttctttcta tcggaacatt aaacagttgc tgattataca attctgtctt
      601 gattcgcaaa taggaagtta cattccagtc gtgcgttaat acgatgtggt ggctattgga
      661 aaagccaagt gatttattgg tctgcaccgc accgtccgtg gtgttggtct gaacaaaata
      721 agtataaaca ctttgcatct gtgcgtgata accgtatcct gcacttagcg ccattccttt
      781 tctgaaattc catcgcaatg ccaatcgcgg ttccgcgctg atactgttac tgatgtttaa
      841 caactgacta tgtatcccgc ccaccaatga gaagcctttg ttgaaccggt gcttccactg
      901 aacataggtt tgacccaata tgtaaacgtc gtcggaatcc acacgaacaa catccgtaac
      961 tccacctaaa acgactttat tgaacaaacc aaacttaacg taatctgtca ttacaccacc
     1021 aacaatagta ttgcgtgcgt tcagcttgtg attaacaata aaatgtgctg aaaatttatc
     1081 cgtatcaaac cgcgcttcac cacttggaaa ttcctcgcgg gtgatgatgg aaatggaatc
     1141 tcctttaaat cgttcatagg tacgactatg gcctgctacc aaacgcaagt tggttttgga
     1201 acttaactgg tgattataac ttaaaccatt gatgtaggtt gcataacgaa cgcgtgtatt
     1261 ggtattttca tctccataca aatcaatctg agtagtatcg acatcattcc ccagaaaatt
     1321 cacgctgctg ttcccattta taccaaaaaa agtaagctgt ccatttttac caaccgggaa
     1381 ataaaattta taattgatat cctggtaaag aggtgttgct gttcctgtac caaagttgat
     1441 tcccatttgc tgaaaaacac ccaatgtaga gtaacggtaa ttcaaaaggt aacttgattt
     1501 tttctttttc cccatcgggc cttcagcacc aaattcgaat ccgttaaaac cgatctgacc
     1561 aagaaattcg tgtttctcag tattcccttt tcgtaaatga agatcaaaaa ctccggccag
     1621 tgcattgccg tattgcgcag gaaatgctga tgtcaaaaaa tcggatttat ccaggttgtt
     1681 gttattgatc atgctaaccg gtccgccggt agaattcaat gctccgaaat ggttgggatt
     1741 gggaatgttc agtccttcca tttgccataa catgcctgaa ggagaatttc cgcgcactac
     1801 aatatcattg cgcgaatcat ttcccgcaac tactcctgca aaatttgcag ccattcggga
     1861 aggatcaccc aatgaacctg catattttct ggtttcctcc aaattaaagg aacgtgctcc
     1921 tacaattgtc atttcgttta ccgactgatc cttatccgcc gagcgatcgt aaaccacatc
     1981 aactgtttca agcatttcaa ccgattcagt catggaaaca gttaacacca cttcctttcc
     2041 tgcagtaaca ataaggtcgg ttaatgtgcg atcttcataa ccaataaaac tgaattttag
     2101 tgattgacgt cccaagggaa cattctgcaa cttaaaatat ccatttccat cggtggaagt
     2161 acctaccgga ggatcggtac ctattaaaac aatgttaaca ccgggtaagg gagcttttga
     2221 tgcctcgtca ataacctgac cgcgtatggt ttgctgtgct tgaagcgtac tcatccaaaa
     2281 aagagcaagt acaaaaagta aagtgttttt catatgcatt gatttctgca gcaaacatcg
     2341 tttcaattcg caacatatca ccttgactca aatcaagaag aaaggtaatt gcagtttttc
     2401 ttgatttgaa tcaagaatgc catttgaacc ggcttcagga attctgcttt gctttttcgg
     2461 taaggcgttt tcttattctg ctcagcgttt cgggagtaat acccagataa gaagcgatca
     2521 tatataaagg tacccgctga aacatttgcg gatgagcttc gaaaaattca ttgtaccggt
     2581 cttccggtga ttttgtcatt aaagtggaat tccgggtgga gagggaaata aaaagtcctt
     2641 ctgctatcag acgccccatc cgttcgaaca atgcaccgcg gctgtaaagg gattgcattc
     2701 cttcataatc catttccacc aaaatagaat cttccagtgc ctccagataa tccatggtag
     2761 gccgacgggt aaggaaggaa cagtattctg aagtatagca attctccgga caaaaatcgg
     2821 cagtgatttc tttgccttcg ttaaaataaa aataacgcac taatccttcg gctacaaaag
     2881 aaacatgatt gcagacttcg ccgggtttaa cgatcatttc acccttctta actcgacgaa
     2941 cccgctgtac cgattgaaaa aaatgccaat cctcttccgt catggacgga ttgagttgct
     3001 gataataagc ctttaatgcg ttaataggat ccatactgcc aatataggac gaaaatcctt
     3061 atttcatatt gcatgattcc tcagatttcc tcaaacagga actaaactct ttgtagtttt
     3121 gcagcctatc attttgatta tggctacagc accaaaaacg gttaagaaaa gcattctgag
     3181 tacggcagat tttaaaaaac ttgttttgca gggaattccg gatcgtttac ccaaagcaaa
     3241 aacatgggat cctgagatca atcatgcacc caagcgcaag gaaatattat ctgcagaaga
     3301 aaaaaagctg gcgctgcgaa atgcactccg gtattttccg aaaaaacatc atgccgtttt
     3361 agcaaaagaa tttgcagaag aattaaaatc atacggacgc atttatatgc atcgttttcg
     3421 tccggattat gaaatttatg cgcgacccat tcagcactat cctgcaaaaa cgaaacaggc
     3481 tgctgcaatc atgcatatgc tgagtaacaa tctcgatcat gcagttgccc aacatccaca
     3541 tgaactgatt acctacggtg gaaacggtgc cgtgtttcaa aactgggcgc aatacctgct
     3601 caccatgcaa tacctttcaa ccatgacaga agaacaaaca ttggtactgt atagtggtca
     3661 cccgatggga ttgtttcctt cgcacaaaga tgctccacgt gttgttgtta caaacggaat
     3721 gatgattccg aattacagca aacccgacga ttgggagcga ttcaatgcac ttggcgtaac
     3781 ccaatacgga caaatgacag caggttcgtt catgtatatt ggtccgcaag gaatcgttca
     3841 cggtacaacc ataacggtaa tgaatgccgc ccgaatgaaa tcgaagggaa aagattacaa
     3901 gggaatgttg tttgttaccg caggattagg cgggatgagt ggagcacaac ccaaagcagc
     3961 caccattgca ggcgttgtga gtataacggc agaagtaaat cctaaggcaa cctataaaag
     4021 gcatgaacaa ggctgggtag atgaagtata tgcagatgtt gacaaggcga ttgatcgtgc
     4081 agaagaagcg cgcagtaaaa aaatggcatt atccattgcc tatgatggga acattgttga
     4141 tttatgggag cggcttgcgc aacgaaacgt caaggttgat ttaggatcag accagacatc
     4201 attgcacaat ccctgggcag gaggatatta tcccgcagga atttcttacg aagaatcgaa
     4261 ccgcatgatg gcggaagagc ccgaaaaatt taaggaagaa gtgcagaaaa ctttacgccg
     4321 tcatgccgca gccatcaaca aactaaatgc acagggaatg tactttttcg attacggaaa
     4381 cgcattcctg cttgaggctt cacgcgcagg aggagatgta aaaggaactg atcgcgagtt
     4441 ccgttatcca tcgtatgtgc aggatatttt aggtccgatg tgtttcgatt acgggttcgg
     4501 tccattccgt tgggtttgca catcggcaga ccccaaagac cttgaagcaa cagataagat
     4561 tgcattggat gtgatgcgaa aaattgctgc gacagcccct atggagatca aacaacaaat
     4621 gcaggacaat atccgctgga tagaagaagc gcagaagaac aaattggttg ttggatcaca
     4681 ggcaaggatt ctttatgccg attcggaagg aagaataaaa attgctgagg cattcaacaa
     4741 agccattaaa gccaaaaaaa ttaaatcacc aattgtattg ggacgcgacc accacgatgt
     4801 ttccggaacg gattctcctt atagggagac atcgaatatt tacgatggtt cgcgatttac
     4861 agccgatatg gcggttcaca attttgtggg cgacggattt cgtggagcca catggatatc
     4921 gctgcacaat ggcggtggcg taggatgggg agaagtgata aatggcggat tcggaatggt
     4981 gctcgacgga agcaaagatg ccgatcgccg gttgaaaaac atgttgttct gggatgtcaa
     5041 taacggaatt gcacgcagag cctgggcaag aaacaaggaa gcgatgtttg caatacagcg
     5101 tgaaatgaaa cgaagcaaaa aattaaaagt aaccctgccc aatctggtcg acgataaaaa
     5161 tctggaagga cttgtctaat cgcctttcct ccgttttttc cctattaagc aaaaaaagag
     5221 cctccatgga ggctcttttt gtagtgtgga aatacagaat cagatcaggt atttttcaac
     5281 gaaatcttcg actttcattt cagtagaatc aacagtaact gtctgaacat tcagcgttac
     5341 gaaataacgg tgcattacgc gacgtgtttg tgattcgttt ttgtgcatgg tgatgctcag
     5401 gctgatggat ccttttcctt cttttgcagc aacggtgaac catgaagcat aggtttttgc
     5461 attttggtta acacttgcga tgtcaagccc ggtagcagta aattcaaata caccggttga
     5521 agcttttgcc ttacctaaag cagcagaacc gccagtggca gtaagattct gactgaaacc
     5581 aaatgttgcg atcagaacaa agacgagggt agaaagtagt tttttcattt tggaggtttt
     5641 aagaagggtc aatttagtga atttcacaac aaacagcaaa attcaggcca aacaaaacac
     5701 ctaacaccct tggaaaccaa gcacggaaga gtatttttgg gcatggaatg gcaggaagtc
     5761 tttttttcga cctggtttga gtggatgggc tatccggtga gcggactgga gttcattgga
     5821 agcattgcag gattagtggg agtttacctg gctgcaaaat ccaacattct tacctggccg
     5881 gtgggaatag ttaatgtggt ttgtttcttt tttgtttttt ataccgcccg tttgtattcc
     5941 gacatgctgc tgcaattcta ctatttttcg gtgagcatta ttggatgggt aaactggaat
     6001 aaaagcggag aacaaaaact taaacatgaa tggatcggtc aacagtggcg attccgtctc
     6061 cctctgatta ccattgttgc aacgctggga ataggatacc tgatcgagca cctgcacatc
     6121 ctttttcctt cagtgctgca tgaacccgcc gaccttccct atctcgacac gctgattggt
     6181 gttttaagta ttcaggcaac gtttttacag gcacgaaaaa aaattgattg ctgggtaatc
     6241 tggattgtaa ccgacgcatt atgcaccggg gtttattgga aaaaaggaat acatcttgtt
     6301 tcgctggagt ttctgctatt tactatgctt gcaattttcg gattaatgca gtggctgcaa
     6361 aaacaccgcg aagaattagc cggaaaataa attctgtcag gagacacttg tttccttctt
     6421 ctaacgcgct tcaacatcca ctgtgccgga acgcaaaaaa ctgcggatca attcatcagc
     6481 atccggaaaa taatcaaagg gaaccacccg ctttctgtat tcctgcagcg aatcgaaagt
     6541 gacatccaca ttcaggtaac cgtatcccgc atactttcct tcgagcatca gtacaaatga
     6601 atattcatgg gagttcctgc ctgctccttt tatcaattga tcggatttat cacctgcaca
     6661 ggtttcgatc agattctgtg tccgtgcatt atagatttca ggatcttctt ttccgcaaca
     6721 gattccgttg cattttctaa tttgaaaatg aaagcatggt ccttccatat caaaagctcc
     6781 gcaaaatttc ggacatacat attcgttgtc catccaatta tttaaaaatg cacgcgcact
     6841 gatccaggta tgaaaaattt ttagtggctc tttactatca gctgttggaa caatctgaaa
     6901 ctgcaaaaca ccattatcat gaattgcaga ttcaagcgcg taggcaaatg cctttttacg
     6961 acgtgcacgg ttatagggag gttggtgacg tttaatctct tctgattcga gcaacagcgc
     7021 aatcagttca gagcccgtct cttcaaattc tacattgtac acctctccaa tcatcttgcg
     7081 cgctttcttt tccttcgact gataatgctg aatggcacgc tgccgcatat tggtgctttt
     7141 gccgatgtac agaatatttt cgcctttacc taaaaagcga tagacacccg ttttctccgg
     7201 aagttttttt agaatgtact gctggatctt ttgtgttctt ccggtattga ttgcttcaag
     7261 attttgaagt ttatagaccg gatgcgcact ttttaattgc atcagatagg aaaacagaga
     7321 agcggttgcg tctgcatctg ccaaagcacg gtgcgcatta tgaaggggga tctgcaatgc
     7381 ctcgcacaat cgtccaagcg cataggattt tcttccgggg atcaattttt tacttaatcg
     7441 tacagtacac agtttatcgc gtttgtagga atatcccaac gagcggaatt cttcctgaat
     7501 aaagcgaaaa tcaaattcta cattatgagc aacaaaaatt ttgtcgcggg ttagttccag
     7561 tatttttttt gccacctcat aaaacttcgg cgcctctgcc accatctcat tggtgatacc
     7621 cgttattctg gaaattgttt ggggtattga acgctcggga tgaatcagcg tgctgtatcg
     7681 ttcaacaacc gttaatccgt cgtgcaccac aatagcaatc tcagtgattc tgtcgcgttt
     7741 gggatcgagt ccggtagttt ctatgtcgat gatgctgaac acaaatagat cacaatttac
     7801 gcgattttaa atccggagca gaggaaccgg taaattttag caaagtgcaa aagcagaacg
     7861 cagtctatct gcgttataaa taatttacag cgcatatcca ctgcgtgctg atggtgaaaa
     7921 tttgcatcat caatagttgt ttaatcaata cctcaaatca tgaacgcagc agcactcccc
     7981 tttggaagaa cccgtcaggc tcagcaccag ttcctgcccg aaaaaaagag caatctccgt
     8041 gcggtaaaat cggacgataa agagatcatg cttcaaccct tccgcgattt agctagtgcc
     8101 atcgttgaaa tggggcaatt caaactcagc ggcgaacgcg aattaagcga aggcaacaaa
     8161 agcctgctgt ttcttcgcaa tacaagagaa gaccttcata tcaaaggcga acctgtaaaa
     8221 gaatacctcc tgatcgtggc ttcgcatgaa agcagtggta aatcttttct ggctacttac
     8281 catataccgg aaaaccgcgg gaacaggtat tgccataaac tgcaagcact ctttgcacaa
     8341 cagcttagtc cggctgaact gcagcagcag gcacttcgca ccatacgtaa ttacgaatcc
     8401 ggtcgtcgcg atctttatca gttgcttcac gcattttcta cagtccgcgc cgatgaagaa
     8461 ctagtggaag aagtgcgtaa cgaagtggtt cgttacctgc aacaaacctt acgcaacaaa
     8521 cccacgctat ccggcaaagg catcgtgaca caaatcaatg cgctcgacaa tgtaatgtcg
     8581 gtatgccggc attcgcgtca ccacacgctc aaaacgctgt tcgacagtat taccaccttc
     8641 agtacctatc atctcgacat ccgcgaacct gcacaggcgg aaggattggg tgcagatttg
     8701 aaccagcata tttttcatct tctggaaaga gagtggcaag cggagctggc agaaattcat
     8761 ccataaaaaa gccccgaacg ttcggggctt gaataatcag ttgatgtttt tggcaggacg
     8821 accacgtttt ccggtagatt tttttacacc gggtcgacgc gaacctattc cgaaaacttt
     8881 acggagcaga ctattgtcgg caatctcctg caatactcct gtcattttac ccaggcgttt
     8941 ttcaagtttg gcaactttct tttccagtaa ttgaacatta ggcgcaaggt ctttacgggg
     9001 gcgaccacgt tttcctttcg aaactattac aacaggacca atggccttgt tggcattagt
     9061 g
//
LOCUS       DAOMYG010000062         9050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2558_length_9050_cov_9.116176, whole genome shotgun sequence.
ACCESSION   DAOMYG010000062 DAOMYG010000000
VERSION     DAOMYG010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9050
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2558_length_9050_cov_9.116176"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PK610_03255"
     CDS             complement(<1..322)
                     /locus_tag="PK610_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34972.1"
                     /translation="MIESLKPGTINKEDQTQQPDSFTTKEIDKKLKKKKLKKRKVDLV
                     VISDVHLGTYGCHAEELLRYLKSIKPKKVILNGDIVDIWQFKKSYFPASHLQVVKHLT
                     GLITK"
     gene            734..2725
                     /locus_tag="PK610_03260"
     CDS             734..2725
                     /locus_tag="PK610_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ34973.1"
                     /translation="MRRYIFFFCGFFLFVFSASAQQSDAFKKALADARDCSEAYDFKC
                     AESNYKIAVGLDPHHHAATAEYGILLSKYLNKRKDAFPVLETNLKNSKKGDTIPEVLI
                     EYGRCCHYMGKYTEAIAGFKAVLPFCTNTKSGNELRTLMNKYIQQCEYGQANSEGGED
                     LVLIHLNDAVNSTGSEYVPIYLPKDSTVLFTSRRSTGSGLIDGKDGKYYEEIYESKES
                     EGDLGTARKVNLKLSSKVPQHASVSSVSPDGNTIFLVINGKLFSSTYKNGELTEPVDL
                     GPNINMSKYQNHICMTADGKTVYFSSDSKNGQGGLDIWKSTKQSDGTWGKAENLGPDV
                     NTPDDEESPAILPDGKTLYFSSRGHRGFGGYDLFRSDWDGTKFTSVSNLGAPVNSPGD
                     DTYLILEQDGTFGYFASDRASGYGDMDIYILVFPQEDQEFACDFSDHISIKTDQKSKK
                     QRLILSAVLDDGLKAVEYAWKIDGRLIKKAKGDVLDTLFTDGKKHSVHVEARVVSGSR
                     IVLYCADAEVEHQPEALIAVTNETTITTVNTTTETKSNPEPKLITVYFDYDKSGIRPD
                     AASTLSQNAKVLKSNRELKVAVSGHADSRGTDEYNEELSRRRVNAVVNYLVSLGVSRS
                     QIITITAKGESELLNNCSDTVPCSDDDHQKNRRVEFKIQ"
     gene            2835..3224
                     /locus_tag="PK610_03265"
     CDS             2835..3224
                     /locus_tag="PK610_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34974.1"
                     /translation="MKIKQLTLLLFAVFASTLAVGQVQTTNSKLKQPADNTTAKPLGS
                     SGYTSSQQQILKMDVLAEDMKKSVELSQAVSSSDRSSYSAALQKRKEHNKLYAVKLKA
                     ELAKHRQGTPSHSAIQDELNRIAADLN"
     gene            3231..6404
                     /locus_tag="PK610_03270"
     CDS             3231..6404
                     /locus_tag="PK610_03270"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HRJ34975.1"
                     /translation="MKKLYTLIAAIAITGTAYAQPLTENFDDISQLVDWYAQNNSQPV
                     GVAGQGGWGQPGGAPPFNAFNGVANAYVYANFQAGSGVSTLSNWMFMPARYLQNGDVL
                     TFRTRKAAPDAFADRLEVRLSTNGNAMNVGTTATSVGDYTTVLLTINPTLITGVYPTA
                     WTQYTITISGLAQPTVGRIAFRYFVTNGGPSGANSDYIGIDNVVYTPVSAPTNDNCAG
                     ATSIPHGVNCTPVNGNIGGATNSGITACSGTSEDDVWFSFVATTPGAHIQVDGATGMD
                     AVVQVFSGSCGTLVNVTCQDATFDGGVEDIYLPNLTVGQTYYIRVHDYFNYVPNNTTF
                     TICVEQYTPCSLTAPGGAITETELCGADQNGGCLMPTPAYQSLNCGQTVFGNGYATNG
                     NRDTDWYSFTLNQTGNVSFSVQAEFPVDLFFVNISNCASPTVLASTTGAPCGTVTLNF
                     NFTTAGTYAVVVAPSTYDGYPCGTFNDYIGTLTLPTTIPGATAGGATSFCPGGNVTLN
                     GTGTGTYQWNLNGTPISGATAANYSANAAGSYTLALTNSNGCVGFSGSIPVTILPLDN
                     AVFTYASNTICTGSANSTPTVSVPGSFGASPAGLNFVSATTGEVDMTTSVAGSYTVTY
                     TTTGTCPNSSTQTINITNSPDATFSYSAAAYCGNGTDPLPVFGGGASSGTFTSAGGLS
                     IDAGTGEIDLAASAPGNYTVTNTIAASGACPQVTANFAVEVYAVPTAVVSGGGNVCND
                     GISTAPVTITLSGNGPWDFAYSDGVSPFTVNGHATNTYTIAAGTGSFTMTSVSDNNCS
                     GTVSGSATINVISLPTAVINGGGSACVGETVDVTVQLTGTAPWNFVITDGTNTFPVTG
                     ENSATHIFSATAAGMYTLVNLSDANCTGTVSGASIVSFNSLPTVALSSQGTTCVNYAP
                     IALTGGTPAGGTYSGPAVTAGSFNPAGAGVGTFSITYTFTDGNGCTNSASQNIVVDAC
                     LSVDEIESTIANLYPNPAADYVIIEGSSSIESLSLMDLSGKVISTQAFNSLNARLDLS
                     SVSSGIYFIEVITANGDHAVRKLVVNK"
     gene            complement(6522..7400)
                     /gene="sucD"
                     /locus_tag="PK610_03275"
     CDS             complement(6522..7400)
                     /gene="sucD"
                     /locus_tag="PK610_03275"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRJ34976.1"
                     /translation="MGVLVNKNSKVIVQGFTGSEGSFHAGQMIEYGTNVVGGVTPGKG
                     GTTHLDRPVFNTVQDAVEKAGADVSIIFVPPAFAADAIMEAAAAGIKVIVCITEGIPV
                     NDMVKVKEFLKGRNITLIGPNCPGVITAGEAKVGIMPGFVFKPGRIGIVSKSGTLTYE
                     AADQIVKAGMGVSTAIGIGGDPIIGTPTKEAVEMLMNDPETDAIVMIGEIGGNYEALA
                     SRWIKENGKKPVVGFIAGQTAPKGRKMGHAGAIVGGAEDSAEAKMKIMAECGIHVVAS
                     PAEIGAKMAEILGVTA"
     gene            7570..8949
                     /gene="mnmE"
                     /locus_tag="PK610_03280"
     CDS             7570..8949
                     /gene="mnmE"
                     /locus_tag="PK610_03280"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRJ34977.1"
                     /translation="MKKSATICALSTAPGSGAIAVIRVSGPASVAVVNGVFSKEISNA
                     GGYTAHYGQIQREGVIIDDVVLTVFRSPQSFTGEDSVEIACHGSKYIQQQLLQLLYEH
                     GCDPATPGEFTKRAFLNGKMDLSQAEAVADLIASESAAAHRLAMYQMKGGFSGEIGKL
                     RDQLIHFASMIELELDFSEEDVEFADRTQLRTLVVGIRSIVTRLVDSFAFGNVIRNGI
                     PVAIVGVPNVGKSTLLNALLNEEKAIVSEIAGTTRDSIEDVMMINGVEFRFIDTAGLR
                     ETNDIVENIGIERTWKKVNEADILLYLVDAVDSSSEEIEKVVKGFREKVGNESKKLIV
                     VANKTDKTPDTGNLSNKFGSIPEVIFISAKQGTNIDMLKEALFSYVSSNSFFGQDTVV
                     TNARHHAALQRAQLALQEVEQGMDAGLTGDLLAEHLRMALFHLGEITGQINTEDLLGN
                     IFSKFCIGK"
BASE COUNT         2566 a         1840 c         1921 g         2723 t
ORIGIN      
        1 ctttagtaat tagacctgtt aaatgcttta ctacttgtaa atgagaggcc gggaaataac
       61 ttttcttgaa ttgccagata tctacgatat ctccatttaa aatgaccttt ttaggcttta
      121 tgcttttaag atatcggagt aattcttctg cgtggcaacc gtatgttcca aggtgtacat
      181 ctgaaattac aacaagatca accttccttt tctttaattt tttcttctta agcttcttgt
      241 caatctcttt agtagtgaag ctgtccggtt gctgagtttg atcctctttg tttattgttc
      301 cgggcttcag tgattcaatc atttttccta ttttgcttca tagtaatagc gaaacaatct
      361 tcccatcctt tgatgaaaca aaaggaccag aatctttaac cacatgtcaa cgattccggt
      421 cctaatgtca gtcaaacgaa aagaatggaa gaaaagaacg cgcttttttg tagaataacc
      481 gattggatat tcaattgtat tgggatgaag attattcgtt gcaatcataa tttttccaat
      541 acaaagattt atttcttatt taaacccggc tttatggctg ttttattgct tggttatgta
      601 ttccatccaa agattacgga attgtaaatt cagatgtgat cgcgaagggt taagttgttg
      661 ttgttcagta gttttattta ttcaaagacc aagtgttttt ttattatttt taggcctctt
      721 atacaatggc taaatgaggc ggtacatttt tttcttttgt ggatttttcc tgtttgtttt
      781 ttcagcttct gctcaacaat cggatgcgtt taaaaaagca ttggcagatg cgcgtgattg
      841 ttctgaagcg tacgatttta aatgtgccga gagcaattat aaaatagctg ttgggctaga
      901 tccgcatcac catgctgcta cagccgaata tgggatcctg ctgagtaaat acctcaacaa
      961 acggaaagac gcatttcctg ttcttgaaac caacctgaaa aattccaaaa aaggcgatac
     1021 catcccggaa gtactgattg aatatggccg ttgttgccat tacatgggga aatatactga
     1081 agcaattgcc ggttttaagg ctgtacttcc attttgtacc aataccaaat caggaaatga
     1141 attaagaacc ttaatgaata aatatattca gcagtgtgag tacggacaag ccaactctga
     1201 aggaggcgag gatcttgtac tgattcatct gaatgatgca gtgaattcca cagggtctga
     1261 gtatgtgcct atttatttac caaaagattc tacggtgctt tttacttcgc ggcgttcaac
     1321 aggaagtgga ttgattgatg gtaaagacgg gaaatattat gaagaaattt atgaatccaa
     1381 ggaaagcgaa ggtgacctcg gtactgctcg taaggtgaat cttaaattgt catccaaagt
     1441 tcctcaacat gcttccgtgt cctctgtttc tcccgacgga aatacaatat ttctggttat
     1501 caatggaaag cttttttctt caacctacaa aaacggagaa cttacagaac ctgttgatct
     1561 gggaccaaac atcaatatgt caaaatacca gaatcatatt tgcatgacgg cggatggcaa
     1621 gaccgtttat ttttcctccg attcgaagaa cggacagggt ggactcgaca tttggaaatc
     1681 aacaaaacaa agtgacggaa cctggggcaa agcagaaaat ctgggtcccg acgtaaatac
     1741 acctgatgat gaagaaagtc ctgccatttt acctgatggc aaaaccttgt atttttcatc
     1801 acgtggtcat cgcggttttg gcggatatga tctgttcagg tcagattggg atgggacaaa
     1861 atttacctct gtaagcaatt taggcgctcc tgtaaattct ccgggcgatg atacttatct
     1921 gattcttgaa caggatggga ctttcggcta ttttgcatcc gatcgggctt ccggttatgg
     1981 cgatatggat atttatatcc tcgtttttcc acaggaagat caggaatttg catgtgattt
     2041 ttcagaccat atttccatca aaactgatca gaagtcgaaa aaacaacgac tgattttatc
     2101 tgctgtgttg gatgatggac taaaggctgt tgaatatgcg tggaaaattg atggtcgtct
     2161 gatcaaaaag gccaaaggag atgtattaga tacgctgttc acggatggaa aaaaacattc
     2221 tgtacatgta gaggcccgcg ttgtttccgg aagtcggatt gttttgtatt gcgcagatgc
     2281 cgaagttgaa caccaaccgg aagcgttgat agctgtaacc aatgaaacta ccattaccac
     2341 tgtaaacaca actaccgaaa caaaatcgaa tcctgaacct aaactgatca cggtttattt
     2401 tgattatgac aaatccggta ttcgtccgga tgcagcttca acactaagcc agaatgcaaa
     2461 ggttttaaag tcaaatcgag aattaaaagt agctgtgagc ggacatgctg attcgagagg
     2521 tacagatgaa tacaatgaag agttatcgcg acgaagggtt aacgctgttg taaattacct
     2581 ggtatcactt ggtgtttcac gttcgcaaat cattactata acagctaaag gtgaatccga
     2641 attattgaat aattgttctg ataccgttcc atgctccgac gatgatcacc agaaaaaccg
     2701 tcgtgttgaa ttcaaaattc aataactgaa aggatttagg tgttaaaagg tgcacatttt
     2761 tcaccttagc ttcatttttc gttgttttta atggcattct gatttaattt agtgagctca
     2821 taaaaactac taccatgaaa atcaagcaat taacattatt actgtttgca gtttttgcaa
     2881 gtacgctagc agttggccag gtacaaacca ccaattccaa actcaaacaa ccagccgata
     2941 atactacggc aaaacctctt ggatcttcag gatacacatc atcacagcaa caaattctga
     3001 aaatggatgt gcttgctgag gatatgaaaa agagcgttga actttcacag gctgttagtt
     3061 cttctgatcg ttcatcttat tctgcagctc ttcaaaagag aaaagagcat aacaagttgt
     3121 atgcagtaaa attaaaagct gaacttgcta aacacagaca aggcactcct tctcattctg
     3181 ctattcagga tgaattgaat cgtattgcag ctgatctaaa ctaaagaacg atgaaaaaac
     3241 tatatacact tattgcagca attgcaatta caggtacagc gtatgctcaa cccctcacag
     3301 agaattttga tgatatcagt cagcttgtag attggtacgc acaaaataac agtcagcccg
     3361 taggtgttgc aggtcaaggt ggttggggac aacccggtgg cgctcctccg tttaatgcat
     3421 ttaacggtgt tgccaacgct tatgtttatg caaactttca ggccggttcc ggagtttcta
     3481 ccttaagtaa ctggatgttt atgcctgctc gctaccttca gaatggagat gtgttaacat
     3541 tccgtactag aaaggctgct ccggatgcgt ttgcagatcg tcttgaggtg cgtttaagca
     3601 caaacggcaa tgcaatgaat gttggaacaa cagcaacctc tgttggagat tatactacgg
     3661 tattattgac cataaatcct actcttatta ccggagttta tccaacagca tggacccaat
     3721 ataccattac aatttccgga cttgctcagc caacggttgg gcgaattgcc tttcgttatt
     3781 ttgtaaccaa tggtggacca tcaggtgcga attctgatta tatcggtatc gataatgttg
     3841 tttatactcc tgtttctgca ccaacaaatg ataattgtgc gggtgctacc agtatacctc
     3901 atggagtaaa ttgtacacca gtaaacggaa acataggtgg agcaaccaat tcaggaatta
     3961 ctgcatgtag tggtacttca gaagatgatg tgtggttcag ctttgttgca acaacacccg
     4021 gtgctcatat tcaggttgat ggtgctacag gaatggatgc agttgttcag gttttttcag
     4081 gaagttgcgg aacgttggtc aatgtgactt gtcaggatgc aacgttcgat ggcggggttg
     4141 aagatattta tcttcctaat cttactgttg gtcaaaccta ttatatccgt gttcacgatt
     4201 atttcaatta cgtccccaac aatacaacat tcacaatttg tgtagaacaa tacacccctt
     4261 gttcattaac ggccccaggt ggagctatta ctgagacgga attgtgtggc gccgatcaaa
     4321 atggaggttg tttgatgcca actccggcgt atcaatcatt gaattgtggt caaactgttt
     4381 tcggaaatgg atacgctaca aacggaaacc gcgatacaga ctggtatagc tttacactca
     4441 accaaacagg taacgttagc ttttctgttc aggctgaatt tcctgttgat cttttctttg
     4501 tcaacatttc aaattgtgct agtccaacag tacttgcttc cacaacggga gcgccatgcg
     4561 gaacggttac gttgaatttc aattttacaa cagcaggtac atatgcggtt gtcgttgcgc
     4621 catctactta tgatggatat ccttgcggaa cctttaatga ttacatcgga acattaacct
     4681 taccaacaac cattccgggt gcaactgcgg gtggggcaac ttcgttttgt cctggcggaa
     4741 atgtaacact caatggtaca ggaacaggaa cttaccaatg gaatttgaat ggaactccaa
     4801 tttcaggtgc aacagctgca aactactctg caaatgctgc cggtagctat acgttggcat
     4861 taaccaattc aaacggttgt gttggtttct caggttctat tccggttaca atcctgcctc
     4921 ttgataacgc cgtatttact tatgcatcca ataccatttg taccggatct gcaaattcaa
     4981 cgcctacagt ttctgttccc ggaagtttcg gcgcttctcc cgcaggattg aattttgtaa
     5041 gtgccacaac cggagaggtt gatatgacaa cttcagttgc cggatcctat acagttacat
     5101 atactacaac aggtacttgc ccaaattcaa gtacccaaac catcaatatc accaactcac
     5161 cggatgcgac tttttcctat tcagctgctg cgtattgtgg aaatggaaca gatcctcttc
     5221 ctgtttttgg aggaggcgca agtagcggaa cattcacttc tgcaggtggt ttgtctatcg
     5281 atgccggcac aggagaaatt gaccttgctg cttcagcacc tggaaattat actgtgacca
     5341 ataccatcgc tgccagcggt gcttgtcctc aggtgactgc caatttcgct gttgaagttt
     5401 atgctgttcc aacagctgta gtttcaggtg gtggtaatgt ttgtaatgat ggaatttcca
     5461 cagctccggt tacaattact ctttctggta atggtccatg ggatttcgct tattctgatg
     5521 gcgtatcacc atttactgta aacggacatg ctacaaacac gtatacaatt gctgcaggta
     5581 ccggctcatt tacgatgacc agtgtaagcg ataataattg ttctggtact gttagcggtt
     5641 ctgctacgat caatgtcatt tcacttccaa ctgcagtaat caatggagga ggatctgcat
     5701 gtgtaggaga aacagtagat gttactgttc aattaacagg aacagcaccc tggaatttcg
     5761 ttatcactga cggaacaaat acattccctg taaccggtga gaattcggca acgcacattt
     5821 tctcagctac tgctgcagga atgtataccc tggtaaactt aagcgatgca aattgcacag
     5881 gtacagtaag tggagcttct attgtttcat ttaattctct cccaactgtg gcactttctt
     5941 cacaaggaac aacatgtgtg aattatgcac caatcgctct caccggagga acaccagcag
     6001 ggggaactta ttccggacct gcagttactg ccggatcgtt taatcctgcc ggtgccggag
     6061 ttggaacatt ctccattacg tacactttca ctgatggaaa tggttgtacc aattctgcgt
     6121 cacaaaacat tgttgttgat gcgtgtcttt ctgttgatga aattgaaagt actattgcca
     6181 atttgtatcc taatccggct gctgattatg ttattatcga aggtagctca agcattgaat
     6241 cccttagcct tatggatttg agcggaaaag tgatttctac ccaggcattc aattcgctta
     6301 atgcaaggct tgatttaagc tcggtttctt caggtatcta tttcattgaa gttataactg
     6361 ccaatggaga tcacgctgtt cgtaagctgg ttgtaaataa ataatcaaag ctttcatcca
     6421 atagtaaaag ccccgctcgt gcggggcttt tttttattca ttacaaaaaa aaacaccctg
     6481 ctaaacaggg tgttttatat agaaattgga tagtgttttg attaagcagt aacacctaag
     6541 atctctgcca tttttgctcc gatttcagcg ggagatgcta caacgtgaat tccacattct
     6601 gccatgattt tcatcttagc ttcagcagaa tcttctgcac cacctacaat tgcaccggcg
     6661 tgacccattt tacggccttt tggagcagtt tgtccggcga tgaacccaac tacaggcttt
     6721 ttaccgttct ctttgatcca acgtgatgca agtgcttcat agttaccacc gatttcacca
     6781 atcatcacaa ttgcgtcggt ttcggggtcg ttcatcagca tttcaacagc ttctttggtc
     6841 ggagttccaa taataggatc cccaccaata ccaattgcgg ttgatacgcc catccctgct
     6901 ttaacaatct gatcggcggc ttcgtatgta agtgttcccg atttggaaac gattccaata
     6961 cggcctggtt tgaacacaaa accgggcatg atacccactt tcgcttctcc ggcagtaatt
     7021 acacccggac agttaggtcc aataagcgta atgttacgtc ccttgaggaa ttctttcact
     7081 ttaaccatgt cgtttacagg aattccttca gtgatacaaa cgatcacctt gattcctgca
     7141 gcagcagctt ccatgatagc gtccgcagca aatgccggtg gaacaaatat gatagaaaca
     7201 tcagcccctg ctttttcaac agcgtcctga acagtattaa aaaccggacg gtcgaggtgg
     7261 gttgttccgc ctttgccggg ggtgacacca cccacaacat tggttccgta ctcgatcatt
     7321 tgtccggcgt ggaaagatcc ttcgcttccg gtaaagccct gtacaatgac ttttgaattc
     7381 ttgttcacta aaacgcccat ggtgtgtatt tttcggttta tgaattggca gcccaaatat
     7441 atcaatttcc tacacgtcaa tggcacttgc ttttaaagat gaaatccaca gtttggggtt
     7501 aaatagatat gccaagcacc ttgattatca aagaatccat gcagggattt tgtatttttg
     7561 cttttaatgt tgaaaaaatc tgcaaccata tgtgcattaa gtactgctcc cggtagtgga
     7621 gcaattgctg taattcgggt gtccggacca gcctctgttg cggtcgttaa tggggtgttc
     7681 agtaaagaaa tttcaaacgc cgggggatat accgcgcatt atgggcagat ccaaagagaa
     7741 ggtgtaataa ttgatgatgt ggttctaact gttttccggt ctccccaatc ctttaccgga
     7801 gaagacagtg ttgaaattgc ttgtcatggt tcaaaataca ttcagcagca actattgcag
     7861 ttgttgtatg aacacggctg tgatccggca acaccgggtg aatttaccaa aagagccttt
     7921 cttaacggaa aaatggatct ttctcaggcc gaagctgtag cagacctcat agcatcagaa
     7981 tcagcggctg cacaccggct tgccatgtac cagatgaaag gcggattctc tggcgaaatt
     8041 ggtaagctaa gagatcaact tattcatttc gcatccatga tcgaacttga actcgatttt
     8101 tcggaagaag atgtagaatt tgctgatcgc acacaactac gcacactggt tgtcggtatt
     8161 cggagtatcg taacacgttt ggtggattca tttgcattcg gtaatgtaat tcgtaacgga
     8221 attccggttg ccattgtagg agttccaaat gtgggtaaat cgactttgct taatgctttg
     8281 ctcaatgaag aaaaagccat tgtaagtgaa attgcaggaa caacccgaga ttccattgaa
     8341 gatgtaatga tgatcaatgg cgttgaattt cgttttattg atactgctgg attacgcgag
     8401 accaatgata ttgttgaaaa tattggaatt gaacgcacct ggaaaaaagt gaatgaagcc
     8461 gatattctcc tttacctggt agatgctgtc gacagctctt ccgaagaaat tgaaaaagtg
     8521 gtaaagggtt tcagagaaaa agttggaaac gaaagcaaaa agctgatcgt tgttgccaat
     8581 aaaaccgata aaactcccga caccggaaac ctgagcaata agtttggatc aattccagaa
     8641 gtaattttta tttctgccaa acaaggaacc aatattgata tgctgaaaga agcattgttc
     8701 agttatgttt cttccaattc tttttttggt caggacacag tagtcaccaa tgcgcgtcac
     8761 catgcagcat tacaacgcgc acaacttgct ttgcaggaag tagagcaggg gatggatgca
     8821 ggattgacag gagatctttt ggccgaacat cttcgtatgg ccttgtttca tttaggtgag
     8881 atcacaggtc agattaatac agaagactta ttggggaaca tctttagtaa gttttgtatc
     8941 gggaaataaa tcttctttcc ggaagatgta taggtttttt aaaaaataag tgttgtaata
     9001 gtatatatcc gtgaatttgt tttctaaaac gaaattttca atttttagtc
//
LOCUS       DAOMYG010000063         9024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2565_length_9024_cov_7.454008, whole genome shotgun sequence.
ACCESSION   DAOMYG010000063 DAOMYG010000000
VERSION     DAOMYG010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 9024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9024
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2565_length_9024_cov_7.454008"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /gene="def"
                     /locus_tag="PK610_03285"
     CDS             complement(<1..467)
                     /gene="def"
                     /locus_tag="PK610_03285"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010180344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRJ34978.1"
                     /translation="MILPIVAYGDPVLKKVAEEIDKDYPGLQQLIADMFETMYNANGV
                     GLAAPQIGKSIRLFIVDASPFAEDDEDGENEHLKDFKKVFINPIIEDETGEEWGFNEG
                     CLSIPGIREEVFREEKIKISYYDENFNFFEEEYDGFAARVIQHEYDHIEGVLFT"
     gene            653..1927
                     /gene="fahA"
                     /locus_tag="PK610_03290"
     CDS             653..1927
                     /gene="fahA"
                     /locus_tag="PK610_03290"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HRJ34979.1"
                     /translation="MNIRANDPNRRSWVEVPKGSDFPIQNLPFGVFKTQGEKPRVGVA
                     IGNKILDLKALHVLGHLPNQPFNINAFDTDSLNTMMSYGKKIVREFRNRISDLLEENN
                     PELRDNHNHVAQVLVEQAEAEMLLPVQIGDYTDFYSSIDHATNVGKMFRDPENALLPN
                     WKHLPVGYHGRSSSIVSSGTAIHRPKGQSKAPDQELPSFGPCKLLDFELETGFFTFPG
                     KPLGHSISTEEADEYIFGMVLLNDWSARDIQTWEYVPLGPFLAKNFASSISSWVVTLD
                     ALEPFRTKGATQVPAVLPYLSYQGEKNIDIHLEVIIQTPDGNDNTISNSNNKYMYWTI
                     EQQLAHHTVNGCNIRCGDFLGSGTISGPTRDTFGSMLELTWKGTQPLKMKDGSERKFI
                     NDNDTVVMRGYCEKDGLRIGFGEVKTKVLPAI"
     gene            1930..4137
                     /locus_tag="PK610_03295"
     CDS             1930..4137
                     /locus_tag="PK610_03295"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRJ34980.1"
                     /translation="MKPVIDPEKERQEILNAYRGLLRSCRASRDREDTDIIRKAFNLA
                     VEKHKNMRRKSGEPYIFHPIAVARICSEEIGLGATSIVAALLHDTVEDTDLTLEDVED
                     MFGKKVAKIIDGLTKITGFVDNTESIQAENFRKVLLTMAEDVRVILIKLADRLHNMRT
                     LGSMSKEKQLKIASETEYLYAPLAHRLGLNAIKTELEDLSLKFREPAAYNDIQSKLQK
                     TQAVRNRFIAQFVLPIKRQLDKEGFNYVIKSRTKSVFSIWKKIHQKNVPFDEIYDLFA
                     IRVILNSENEREKSDCWRVYSIVTDYYQPNPDRLRDWISTPKGNGYESLHTTVMSPTG
                     KWVEVQIRTKRMDEIAERGYAAHWKYKENNSAESGLDSWLNNIRELLENPQANALDFL
                     DEFKLNLFSEEIFVFTPKGELRTLPSGATALDFAFDIHSKVGESCIGAKVNTKLVPLS
                     HPLNSGDQIEVITSKKQKPKEDWLKFVTTAKARQKIKDALKEEKKIIAREGKEMLIKR
                     LEQYGILFSEKNIRKLEDHFNSSSSTDFYYRIAKGSIELGFLRDLEIKNGELFFPATG
                     EKVTVEDLIKQSGAKDTGNDLLVLGDDLKQIDYKLSPCCNPIPGDDVFGFVTVSDGIK
                     IHRVTCPNAVELMSNYGYRIVKARWKSQEAIEFLAGVHFKGIDDVGLVNKITSVISKE
                     LNINMRSIFFESHDGVFEGKVLLYVHDTHHLDRLMQNLLKIEGVHTVTRIDNQ"
     gene            4217..4684
                     /locus_tag="PK610_03300"
     CDS             4217..4684
                     /locus_tag="PK610_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRJ34981.1"
                     /translation="MSATQTKETVRQIFQGYLEQHGHRKTPERFAILNEIYDYEGHFD
                     IESLYVKMKNKKYRVSRATLYNTIELLLACDLVKKHQFGKSQAQFEKSYSKKQHDHVI
                     CTDTGEVLEFCDPRIQQIKETIEEIFGVEVLHHSLYFYANRKDAGKKTTVKKK"
     gene            4688..5035
                     /locus_tag="PK610_03305"
     CDS             4688..5035
                     /locus_tag="PK610_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRJ34982.1"
                     /translation="MEFSYKLSEQKPVVTATLSGRLMDKQSAQPLLDSLDKYIKAGSN
                     RIILDMSGMDYMNSSGLNVLVNILTKARNQHGEVVICNLSKKIQELFLITKLNTLFQV
                     TTDVTEAVESFKL"
     gene            5066..6337
                     /locus_tag="PK610_03310"
     CDS             5066..6337
                     /locus_tag="PK610_03310"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRJ34983.1"
                     /translation="MKVDVLLGLQWGDEGKGKIVDVLTPKYQIIGRFQGGPNAGHTLE
                     FNGIKHVLHTIPSGIFHDNVINLVGNGVVVDPVVLKREIDALKKLGVDVKKRLLLSRK
                     AHLILPTHRLIDQASEASKGKEKIGSTLKGIGPTYMDKTGRNGLRIGDILLPDFKEKY
                     QKLVEKHIKILSFHQFEYDLAALEPAFFEGIESLKELTFVDSEHYINQALREGKNVLA
                     EGAQGTLLDIDFGSYPFVTSSNTTTAGACTGLGIAPNRIGEVIGIFKAYCTRVGSGPF
                     PTELEDEDGDRLRKIGNEFGSTTGRPRRCGWIDLPALRYAITINGVTSLAMMKSDVLN
                     SFETIKVCTHYKIDGQLSTDMPYDLCSIKAEPVYKEIKGWNCDLNDVETFETLPKELL
                     DYVAFLEKEVGVPITVVSVGPDRTQTLVRKA"
     gene            6343..7686
                     /locus_tag="PK610_03315"
     CDS             6343..7686
                     /locus_tag="PK610_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRJ34984.1"
                     /translation="MFPQLRSLVKSDFPTREVNRLAFPAIVSAIAEPIISMCDTAIIG
                     QYGQPGDAGIGSVTIGTNFFLSIIWVFAQTRTAMSSVVSRYFGEHQLDKIRTLIPQVI
                     FMNFTLGVFFYLVTNLFAVEIFSLYNAEGAVLQQSVDYFSIRSIGFPFTLSTLLIFGV
                     FRGMQNTWISMVISVTGLVLNILLDIAFINGLGDVIPALGAKGVALASVISQIVMFGM
                     AIVMLIRKTPFNLNFLHPVNSEIYSLLKLTGNLIIRSIAVQTAYFFSGRIAASYGEHA
                     LNVHGVFWNIWLFSAFVIEGYCSAGNALAGKYFGEKNFESLWRMSQYIFSRTLLLGIG
                     LMLFYFLVYPFIGHLFLRNEEELSLFLSFFWIVALVQPFNALAFTYDELLKGLGRMRY
                     IRDTLIIATFVGFVPVIHLGYWMDWHLLSVWLAFIVWMSIRGIRLMWYFNRHFRY"
     gene            7836..>9024
                     /locus_tag="PK610_03320"
     CDS             7836..>9024
                     /locus_tag="PK610_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HRJ34985.1"
                     /translation="MNPYRFILFVFCLSISFSSISQTSDSLVNKEIREAEAHLEKLRQ
                     QEIAIKKKIELLKFESIQQALLTAGYPKGHDAVLFVVKHAAYITAYDEKLEQARWVAH
                     IILPDIAEGNFGRSNDFRIDSLVPTGTPEKYDYWNSGYDRGHLAPSADFRWSAVALSE
                     SYFYSNISPQRPELNRERWAELEDLLRAYVMINSRKLFVITGPVVSESKESIGVKNKV
                     IVPKYFFKATIDPSAGTGIAFIMPNGICTKTVLEYAVPIDSVEKLTGLNLFPNLNPAE
                     EKKMESVYDLSIWRIAKEEGNFSPLNPEELPKGKFNTSQAKYHNGEKITVCGKVVSTK
                     YTEKSGATFINLDRKFPDQIFTVQIWKDARTNFSYAPENELQGKTICVTGKITVNQGV
                     ATIT"
BASE COUNT         2727 a         1885 c         1906 g         2506 t
ORIGIN      
        1 gtgaaaagga ctccttcgat atggtcgtat tcgtgctgaa taacacgtgc tgcaaaacca
       61 tcatattcct cttcaaaaaa attgaagttt tcatcgtaat aggaaatctt gatcttttcc
      121 tcacgaaaaa cctcttctcg aataccggga atactcagac aaccttcatt gaagccccat
      181 tcctcccctg tttcatcttc gataatggga ttgataaata cctttttaaa atccttgagg
      241 tgctcgtttt caccatcttc atcatcctct gcgaaaggag atgcatcaac aatgaacaaa
      301 cggatacttt tcccaatttg gggagccgca agaccaacgc cattggcatt gtacatggtt
      361 tcaaacatat ccgcaataag ctgctgcaga ccgggataat ccttatcgat ctcttcagcc
      421 acctttttca ggacaggatc accgtatgca acaatgggta ggatcataaa ttttctttgg
      481 tgcacaaaag tagcagttat ttgcaatttt caccgtccct tttgctcagc gtgaacaacc
      541 tatgactgat ttttattttt tgctccctct tgaaaatcac aaatccaatt tggaacgaat
      601 ttcttctgaa taaatgcggg gaacagttat ttttacaaaa acatctgtca ctatgaacat
      661 ccgcgccaac gatccaaacc gccgctcatg ggtggaagta ccaaagggat ctgatttccc
      721 aatacaaaac ctcccttttg gtgtattcaa aacccaagga gaaaaaccac gtgttggtgt
      781 tgccattgga aataaaattc tcgatctcaa ggcgctgcat gttttgggac accttccaaa
      841 ccagccattt aacatcaatg cctttgatac cgattcgctc aataccatga tgagctacgg
      901 aaaaaaaatc gtccgcgaat tcaggaacag gatttcagac ctactcgagg aaaacaatcc
      961 cgaattacgc gacaaccaca atcatgttgc ccaggtgttg gttgaacaag ctgaagctga
     1021 aatgttatta ccggttcaaa ttggtgatta taccgatttc tattccagca tcgaccatgc
     1081 cactaacgtc gggaaaatgt ttcgcgatcc ggaaaacgca cttcttccta actggaaaca
     1141 tttacctgta ggttaccatg ggcgatcttc ctctatcgta agttccggca ctgcaattca
     1201 tcgtcctaaa gggcaatcca aggcacctga tcaggaactg ccttcttttg gtccgtgtaa
     1261 gcttctggac ttcgaacttg aaacaggatt tttcactttt ccgggaaagc ctttgggaca
     1321 ttctatttca acagaggaag ccgatgagta tattttcggc atggtgctgt tgaacgactg
     1381 gtcggcacgc gatattcaaa catgggagta tgtccccctc ggacccttcc tcgcaaagaa
     1441 ttttgccagc tctatttctt cctgggtagt aacactggat gctttagagc ctttccgaac
     1501 taaaggtgca acacaggttc ctgcagtatt accttatttg agttatcagg gtgaaaaaaa
     1561 tattgatatt catctggagg taataattca gacgccggac ggaaatgata atacgatttc
     1621 gaactcgaac aacaaataca tgtattggac catcgaacaa caattggccc atcatacggt
     1681 aaacggttgc aatattcgtt gcggcgattt tctgggctca ggaacgattt ccggtcccac
     1741 ccgcgatact ttcgggtcga tgcttgaact cacctggaag ggaacccaac ctctgaaaat
     1801 gaaagacggc agcgaacgaa aattcataaa cgacaatgac actgtagtta tgcggggata
     1861 ttgcgaaaag gatggtttgc gaatcggttt tggagaagtg aaaacgaaag tattacctgc
     1921 aatctgaaga tgaaaccagt aattgatccg gaaaaagagc ggcaggaaat tctaaatgcc
     1981 tatcgtggat tactccgcag ttgcagagcg tcccgcgata gagaagatac cgatatcatc
     2041 cgcaaagcat tcaatctcgc agtagagaaa cacaaaaaca tgcggcgcaa atcgggcgaa
     2101 ccgtatattt ttcatcctat cgctgttgcc cgtatctgtt cagaagaaat cggacttgga
     2161 gcgacttcca ttgttgccgc attgctgcac gataccgttg aagacacaga tcttacgctt
     2221 gaggacgtgg aagacatgtt cgggaagaag gtggcgaaga ttattgatgg cctcacaaaa
     2281 atcaccggat ttgtagacaa tacagaatcc attcaggcag aaaatttcag aaaagtactg
     2341 ctcaccatgg cagaagatgt cagggtgatt cttataaaac ttgcagatcg cttgcacaac
     2401 atgcgtaccc ttggctcaat gagcaaggag aaacagttga aaattgcatc agaaacggaa
     2461 tacctctatg ccccactcgc tcatcgtttg ggattaaatg caataaaaac agaactcgaa
     2521 gacctctccc taaaatttcg tgagcctgct gcctacaatg atattcaatc caaacttcaa
     2581 aaaacacaag ctgtacgtaa tcgttttatt gcacagtttg tattaccaat caagcggcaa
     2641 cttgacaaag aaggatttaa ttatgtaatc aaaagccgga caaaatcggt gttttccatt
     2701 tggaaaaaga ttcaccaaaa gaatgttcct tttgatgaga tatacgatct gtttgcaatt
     2761 cgcgtcatac tcaacagcga aaacgaacgc gaaaaatccg attgctggcg tgtttattcc
     2821 atcgtaacgg attattatca acccaatccc gaccgtttgc gcgactggat cagcacaccg
     2881 aaaggaaacg gttatgaatc gcttcataca acggtaatga gtccaaccgg aaaatgggtg
     2941 gaagttcaga ttcgcactaa acggatggat gaaattgcag aaagaggtta tgcggcacac
     3001 tggaaataca aagaaaacaa cagtgcagaa agcggacttg attcatggtt gaataatatc
     3061 cgtgaattgt tggagaatcc gcaggccaat gcgctcgact ttcttgatga gttcaaattg
     3121 aatttatttt ctgaagagat atttgttttc accccaaaag gcgaattacg aaccttacca
     3181 tcgggtgcaa cagcacttga ttttgcgttc gatatccatt caaaagttgg tgaaagctgt
     3241 atcggagcaa aagtcaatac caaactggtt ccattgagtc acccactcaa cagcggagat
     3301 cagattgaag taattacttc caagaaacag aaaccaaaag aagattggtt aaagtttgtt
     3361 acaacggcaa aagcgcgtca gaaaatcaag gatgcgctaa aggaagaaaa aaagatcatt
     3421 gcacgcgaag gaaaggaaat gctcatcaaa cgtttagagc aatatggcat ccttttcagt
     3481 gagaaaaaca tacgaaaact ggaagatcat tttaattcct ccagcagtac cgatttttat
     3541 taccgcattg ccaaaggaag catagaactt ggttttttgc gtgatctgga aattaaaaac
     3601 ggagaattat ttttccctgc aacaggtgaa aaagttaccg tagaagatct gatcaaacaa
     3661 agcggcgcaa aagatacagg gaatgacctc cttgtattgg gagatgatct caaacaaatc
     3721 gattacaaac tgtcgccttg ctgcaacccg attccgggtg atgatgtttt cggctttgta
     3781 acagtaagcg atggaataaa gattcaccgt gttacctgtc cgaatgcagt ggaattgatg
     3841 tcgaattacg gttaccgaat cgtaaaggca cgatggaaaa gtcaggaagc catcgaattt
     3901 cttgccgggg ttcactttaa agggatcgac gatgtgggat tggttaacaa aatcaccagc
     3961 gttatctcta aggaattgaa catcaatatg cgttcaatct tctttgaaag tcacgatggg
     4021 gtgtttgagg gaaaagtttt actctatgtg cacgacaccc atcatttaga ccgtttgatg
     4081 caaaatctcc tgaaaataga gggcgtacac acggttacac gtatcgacaa ccaataagac
     4141 atttgcctca tttctgttga aaagttgccc ttctgtgggg tgctattttt cttactttta
     4201 cccctcattt tcacctatgt ccgccacgca gaccaaagag accgttcgtc agattttcca
     4261 gggatacctg gagcaacatg gtcaccggaa aaccccggag cggtttgcca tactcaatga
     4321 aatttatgat tacgaaggtc attttgacat cgagtccttg tatgtgaaaa tgaaaaacaa
     4381 aaagtacagg gtgagccgtg caaccttgta caacaccatt gaactcctac tggcttgtga
     4441 tctggtgaaa aaacatcagt tcggtaagag ccaggcgcag ttcgaaaaat catattcgaa
     4501 aaagcagcac gatcacgtga tctgtacaga taccggtgaa gtcctcgaat tttgtgatcc
     4561 gcgtattcag cagatcaagg aaacaattga agaaatattt ggtgtagagg tattgcatca
     4621 ctccctctat ttttacgcaa acagaaaaga cgccggaaaa aaaacaacgg tcaaaaagaa
     4681 ataagccatg gaattcagct acaaactttc cgagcaaaaa ccggttgtaa ccgcaacact
     4741 gagtggacgt ctaatggaca aacaaagtgc acagcctttg cttgattcac ttgacaaata
     4801 cattaaggca ggtagtaatc gtattattct tgacatgagt ggaatggatt atatgaattc
     4861 atccggactc aatgttttgg tgaatatcct cactaaggca aggaatcagc atggtgaagt
     4921 agttatctgc aacctttcga aaaaaattca ggagttgttt cttattacca aactcaatac
     4981 gctttttcag gtaacaacgg atgttacaga ggcagttgaa agtttcaaac tttgatcaat
     5041 tattaattcg atatacagac aaacaatgaa agtggacgta ttactcgggc tccagtgggg
     5101 cgatgaagga aaaggaaaga tcgttgacgt tctcactcca aaataccaga tcatcggacg
     5161 atttcagggt gggccaaatg ccggtcatac actggagttt aacggaatca aacatgtatt
     5221 acacaccata ccatccggaa tttttcatga taatgtgatc aatcttgttg gtaatggagt
     5281 tgttgtcgat ccggttgttt taaaacggga aattgacgca ctaaaaaaac tcggggttga
     5341 tgtaaaaaaa cgtctgttgc tttcacgtaa agcgcacctt attttaccaa cacaccgctt
     5401 aatagatcag gcctccgaag cttcgaaagg gaaagaaaaa atcggttcta cacttaaggg
     5461 aattggtccg acctatatgg ataaaaccgg tagaaacgga ttacgtattg gagacattct
     5521 tttaccggac ttcaaggaga aataccaaaa gctggtagaa aaacacatca aaattctttc
     5581 attccatcag ttcgagtacg atctggctgc acttgaacct gcatttttcg aaggaattga
     5641 atcgttgaaa gaactcacat ttgtagattc agaacattac attaaccagg cgcttcgtga
     5701 gggtaaaaat gttttggccg aaggagcaca aggcacatta ctcgacatcg attttggttc
     5761 atatccattc gtaacctcat ccaacacaac aactgctggt gcttgtacgg gattaggaat
     5821 agcgccaaac agaattggag aagtcattgg tattttcaag gcttattgca cccgtgttgg
     5881 tagtggtccc ttccctactg aactggaaga tgaagatggt gaccgtctgc gtaaaatcgg
     5941 taacgaattc ggatcaacca ccggtcgccc gcgccgttgc ggatggattg accttcctgc
     6001 tttgcgttat gccatcacca ttaacggagt tacttctctt gcaatgatga agtcggatgt
     6061 tctcaattcg tttgaaacca ttaaagtttg tacgcattac aaaattgatg gtcagctcag
     6121 caccgatatg ccttacgatt tgtgctcaat aaaagcagag cccgtttaca aggaaataaa
     6181 aggttggaac tgcgacctca acgatgttga aacctttgaa acgctaccaa aagaattact
     6241 ggattacgtt gcatttctcg aaaaagaagt tggcgttccc attactgtag tctctgttgg
     6301 tcctgaccgc acacaaaccc ttgtccgcaa ggcctgacgg ccatgtttcc acagctgcgt
     6361 tcactggtaa aaagtgattt cccaacaagg gaagtaaatc gccttgcatt tccggcaatt
     6421 gtttcggcta ttgcagaacc tatcatttca atgtgcgata ccgcaatcat tgggcaatac
     6481 ggtcaaccgg gtgatgctgg aattggatcg gtaacaattg gaacaaattt ctttttatcc
     6541 atcatttggg tattcgcaca aaccagaaca gcaatgtcat ctgttgtttc caggtacttc
     6601 ggcgaacatc aactcgataa aatccgaacc ctcataccac aagtcatctt tatgaatttt
     6661 acgcttggcg tattcttcta tctggtgacc aatctttttg cggtggaaat attctcactt
     6721 tacaatgcgg agggagcagt attgcaacag tcggtagatt atttcagcat tcgctccatt
     6781 gggtttcctt ttacactttc cacactttta atttttggtg tattcagggg aatgcaaaac
     6841 acatggattt cgatggtgat cagcgtaaca ggattagtat tgaatattct gctggatatt
     6901 gcttttatca acggattggg tgatgtaatt cctgcactgg gagcaaaagg agttgcacta
     6961 gcatcggtaa tttctcaaat tgtaatgttt ggaatggcca tcgtaatgct gatccgaaaa
     7021 acccctttca atttaaattt tctacaccca gttaactcag agatctattc cttgctaaaa
     7081 ctcacaggca atctgattat tcgttctatt gcagtacaaa ctgcttattt cttttcagga
     7141 agaattgcag catcctacgg agaacatgcg ctgaatgttc atggtgtttt ttggaatatc
     7201 tggttgttct ctgcatttgt aattgaaggt tattgcagcg caggaaatgc tcttgcagga
     7261 aaatattttg gcgaaaagaa ttttgaatcc ctatggcgca tgtcgcaata cattttttca
     7321 agaacactct tgctgggaat tggattaatg cttttttatt ttctggtata tccctttatc
     7381 ggacaccttt tcctgcgtaa cgaagaggaa ttgtcgctgt ttctgtcctt tttctggatt
     7441 gttgcgctgg tccaaccttt caatgcgctt gcattcacct acgatgaact tttgaaaggt
     7501 cttggtagaa tgaggtacat ccgggatacc ctgattatcg caacctttgt tggctttgtt
     7561 ccggttattc atttgggcta ttggatggat tggcatttgt tatccgtttg gctggcattt
     7621 attgtttgga tgagtattag gggaatacgt ctcatgtggt attttaaccg ccacttcagg
     7681 tattgattag ccgtaatttt acaattcatt catcgtaagg actcattcac cctacttatt
     7741 aaccgttact ggtacaatga ttgtatatct taccgcatca ggaattcttt ttcattttag
     7801 tttgagctat tttagatttt agaaaaatgt accgaatgaa tccatatcgt tttatccttt
     7861 ttgtcttttg cctgagtata tccttctctt cgatttcgca aacaagtgat tcgctggtta
     7921 acaaggagat tcgtgaagcc gaagctcacc ttgaaaagct gcgtcaacag gaaattgcga
     7981 tcaagaaaaa aatagaactc ctgaaatttg aaagcattca gcaagccttg ttaacagcag
     8041 gctatcctaa aggtcacgat gctgttttgt ttgttgtgaa gcatgctgcc tacattactg
     8101 cttacgatga aaaacttgag caggcacgtt gggttgccca catcattctt cctgatattg
     8161 cagaagggaa ttttgggaga agcaatgatt tcagaattga cagtcttgta ccaacaggaa
     8221 ctcctgaaaa atatgattac tggaactccg gttacgatcg tggacacctt gctccttctg
     8281 cagatttccg atggtcggca gttgccttaa gtgagtcgta tttctactcc aatatcagtc
     8341 cgcagcgccc cgaattaaac agggaacgtt gggcagaact ggaggattta ttaagagcgt
     8401 atgtaatgat taacagcaga aaactatttg tgatcacagg tccagttgtt tcggaaagta
     8461 aagagagcat tggggttaaa aacaaagtaa ttgttccaaa atattttttc aaagcaacaa
     8521 ttgatccttc ggccgggaca ggtattgcgt ttattatgcc caatggaatc tgcactaaaa
     8581 cagttcttga atatgcggtt cctatcgaca gtgttgaaaa acttacggga cttaatctat
     8641 ttcctaacct gaatcctgcc gaagaaaaga aaatggaatc agtttatgac ctcagcattt
     8701 ggcgcattgc aaaagaggaa ggcaatttta gcccactcaa tccggaagaa ttgcccaaag
     8761 ggaaattcaa taccagtcag gccaaatacc acaacggcga aaaaattaca gtttgcggga
     8821 aggtcgtcag caccaaatac actgaaaaat cgggggctac ctttatcaat ctggaccgaa
     8881 aatttccgga tcagatcttc accgttcaga tttggaagga tgcccgaacc aatttcagct
     8941 atgcacctga aaatgagctt cagggaaaaa ccatttgcgt aaccggcaaa atcactgtaa
     9001 accaaggtgt tgccacaata acta
//
LOCUS       DAOMYG010000064         9013 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2569_length_9013_cov_10.601697, whole genome shotgun sequence.
ACCESSION   DAOMYG010000064 DAOMYG010000000
VERSION     DAOMYG010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 9013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9013
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2569_length_9013_cov_10.601697"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="PK610_03325"
     CDS             <1..827
                     /locus_tag="PK610_03325"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRJ34986.1"
                     /translation="EVKSMKAQRVTDALKKALQAELSINFVGNIPGERLRAVFEPESV
                     AGNRTEKKPRVHLERKQWNQESQIFIYEDKKAVQTQAYFFISGKPFDLKMAPEIAAFN
                     KYFGGDMSSLVFQEIREFRSLAYSTWGRYIAPSKAGQSSWFVSYIGCQSDKTNDAVNA
                     MLDLIRNMPQKPERWTSLQSSLIQSAQSERPGFKSIIETIEGWKYRGYNQDPNIEMIA
                     NYKTMEFENVVSFWKNEISQKPITITLVGNTGAFDMKALEKIGKITLVKKEQMMKK"
     gene            887..2218
                     /locus_tag="PK610_03330"
     CDS             887..2218
                     /locus_tag="PK610_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34987.1"
                     /translation="MEKKARIIASLDHFLDDQQQKLLADALQETPPVSIRIHPLKKFF
                     PENEKQVSWCSLGYYLSERPVFTRDPLFHAGCYYPQEASSMFLYHVLSEILPENPVRI
                     LDLCAAPGGKTTLAATLLRENDLLIANEIERPRCSVLRENVLKYGDPRVYVTNNAPEN
                     FSALEGFFDIIIVDAPCSGEGMIRKDPRALEEWSETNVRTCTVRQHDILQKIWPALKE
                     GGVLVYSTCTYNTDENEKQIENWISNHSAENIELNVPDEWNITKTKAGDVSCYRFFPH
                     KTRGEGFFLSALRKTETCNDHYIRKKDMVSPTREKEIAEWVLPQWQKFLIRNGDSIVL
                     QTHSTEIVRLKKHLRFALESYPVAVKKGKDIIPQPEFAMNIALNPDAFQSLELGQDEA
                     LRFLKTEDISTQGKPGWNLITYKQTPLGWIKQLQQRSNNYFPKEWKIRMEL"
     gene            complement(2234..3499)
                     /locus_tag="PK610_03335"
     CDS             complement(2234..3499)
                     /locus_tag="PK610_03335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRJ34988.1"
                     /translation="MAEIIRMPKLSDTMTEGVVAQWHKKVGDAVKSGELLADIETDKA
                     TMEFESYQNGVLLHIGVEKGKGAPVDSILAILGKQGEDISALLEEEKKKAVAPQAEVK
                     AELPVAKEEPVKIAAEAPKTVSAPVQKTVKTSAPVANANGRTKISPLAKRLAEEKGLN
                     PNLLQGSGDGGRIVKRDIENFGGMGMMSTGVESYTEVAVSQMRKTIARRLAESKFTAP
                     HFYLTLEIDMDRAIDARKAIADISGVKVSFNDMVIKAAALALKKHPAVNSSWLGDRIR
                     YNQHVHMGVAVAVEDGLLVPVVRFADTKPLSQISAEVRDYAQKAKDKKLQPSDWEGNT
                     FTISNLGMFGIEEFTAIINPPDACILAVGGIKQVPVVKNGAVVPGNIMKVTLSCDHRV
                     VDGATGAAFLNHFKQLMENPAVMLGQFSI"
     gene            complement(3514..4569)
                     /gene="pdhA"
                     /locus_tag="PK610_03340"
     CDS             complement(3514..4569)
                     /gene="pdhA"
                     /locus_tag="PK610_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HRJ34989.1"
                     /translation="MASETKAKKSTPGKLVATADKKVKFSKETYVKWYEDMLVMRKIE
                     EKAGQLYLQQKFGGFCHLYIGQEAVVAGTASACRPGDKHITAYRDHAHPIALGVHPKY
                     MMAELYGRSTGMSKGKGGSMHMFHKESNLMGGHGIVGAQIPMGAGIAFAEKYLGTDNV
                     CFASMGDGAVRQGALHETFNMAMLWKLPVIFIVENNNYAMGTSVERTTNVTDLSKLGA
                     SYEMPSFNVDGMSCEAVHLAIEEACERARRGDGPTLLNIMTYRYKGHSMSDPQKYRTK
                     EEVEEYKRQDPIEQVLATIRANKYLSEKEIEAIEQKVDAIVEESVKFAEESPLPDPSE
                     LYNDVYAEPNYPYIIEY"
     gene            complement(4609..5115)
                     /locus_tag="PK610_03345"
     CDS             complement(4609..5115)
                     /locus_tag="PK610_03345"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRJ34990.1"
                     /translation="MSSRVQKVEFQYESFDSRDELAQDEQELIAKAVEAAEMAYAPYS
                     RFHVGAALRLSGGRIVIGSNQENVAYPSGLCAERVAFFAAGAQFPGKTIEKVAIVAIS
                     EDFRVDEPVAPCGACRQSMSEYEIRQDQPIRLLLSGSSGKVLVIDSVLSLLPLVFNET
                     KLKKSPAQ"
     gene            5203..6771
                     /locus_tag="PK610_03350"
     CDS             5203..6771
                     /locus_tag="PK610_03350"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRJ34991.1"
                     /translation="MIAPERSFISFNLIGGCSRFILLFAALCISPLYTQAQNAGDLFF
                     GAATVHEIRLTFHQNQYWDSLTAGYANDVYIKADVEIDGSMLANSGAKFKGNSSYNNP
                     SNKKSFKLDFNEYIPGQKYNGLKKLNLNNCFKDPTFLREKLMLDFLGHRGVPAPRCTY
                     AELYINNVRWGLYTVVEEIDKTFLKRVFNEDKGNNFKGDPSGDLKWLGPAASNYYPKY
                     ELKTNETLNNWNDLILFINKLNMTSTAVLPDTLANYFNLEEYYTTWASHILFSNLDSY
                     LGSGHNYYVYHDSLSNKFRFITWDVNEAFGNFNMGMNVSQIESLSIFFTPNPAGNRPL
                     HERLLQNNSVKQRLADELCAMVNYDFSIWTLENKIDSLVTVIRPYVYADNLKFFSNQN
                     FEDNIDQTITVSGVPGGNTLPGIKSFLINRRNSVATQLAAWSCTVGVEDANPTELTAF
                     PNPGNGMLQILGPEGSSWDYSVFDLNGKKMSGGQFLLPGRINTESWDSNGLFLIRFNN
                     QQTNSSYVVKFLKL"
     gene            6786..7172
                     /locus_tag="PK610_03355"
     CDS             6786..7172
                     /locus_tag="PK610_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34992.1"
                     /translation="MKRILFPSLILLSILFTSCKKEEGVGGMATIKGKIWMKEYTGGV
                     LIDQYFAPDHDVFIIYGEGNTYYDDKISTSYDGSYEFRYLRPGRYKIFTYTKHEITGN
                     LSPEIRSVEISGKKDVIVLDDITVKD"
     gene            7169..7918
                     /locus_tag="PK610_03360"
     CDS             7169..7918
                     /locus_tag="PK610_03360"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HRJ34993.1"
                     /translation="MTLSSSIIEQFDAISLDEMDSVKLMNRTDTKFAFNSERLDEILS
                     LCKNDYRVLAINGKRSASYITLYFDTPDHSLYLAHHNGKPYRYKIRIRNYVESGLFYL
                     EIKQKIKGRTVKSRIKLKGFEEELSDRSKEFIEKTIGKKLELRPALWNNFRRITLVHC
                     VAKERVTIDTSLTFSLQNTSRETSELAIAEVKQENRNRNSGIIQILRKLGIRESGISK
                     YCVGSALIKPQLKYNTFKEKILFIDRLNKVA"
     gene            7923..8681
                     /locus_tag="PK610_03365"
     CDS             7923..8681
                     /locus_tag="PK610_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HRJ34994.1"
                     /translation="MLIDFFNDFLFQLQTDDLSTIDLPTAKLKAKAVKDSLLGMPIYD
                     DDFWKMIIRFAINLTVLVVMIRLVYYPTSKRKDYLFTYMMIGFISFFICIALKNKDID
                     TGMALGLFAIFSIIRYRTDAIPIREMTYLFVVIGISVTNALTGKDSSLVGLLFINLTL
                     LILVYGFERIWMLKHESSQLVIYEKIELIKPQKHQELIQDLEDRTGLKINRVSIGKID
                     FMRDIAQIMIYYYESDQRNISNYSASSGSDDGDD"
     gene            complement(8712..>9013)
                     /gene="ispF"
                     /locus_tag="PK610_03370"
     CDS             complement(8712..>9013)
                     /gene="ispF"
                     /locus_tag="PK610_03370"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRJ34995.1"
                     /translation="HHFPDTSNEFKGIDSKILLKRTYALVREKGYRVNNIDSMLCLEK
                     PKIKPHIDAMRESIASILEVEMDDVSIKATTNEKLGYVGKEEGLTASAVVLLIGV"
BASE COUNT         2701 a         2056 c         1791 g         2465 t
ORIGIN      
        1 gagaagtaaa atccatgaaa gcacaacgcg ttaccgatgc actcaaaaaa gctctacagg
       61 cagaattaag catcaatttt gtcgggaaca ttcccggcga acgtctgcgt gcagtttttg
      121 aaccggaaag tgtggccgga aacagaacag agaaaaaacc gagagttcac ctggaacgga
      181 aacaatggaa tcaggaaagc caaatattta tttacgaaga caaaaaagct gtgcaaaccc
      241 aggcctactt ttttatcagc ggaaaaccgt tcgacctcaa aatggctccc gaaatagcag
      301 cattcaataa atactttggt ggcgatatgt cctcacttgt tttccaggag atccgcgaat
      361 tccgatcgct cgcctattct acctggggaa gatatattgc tccgtccaaa gccggacaat
      421 cttcctggtt tgtttcctat atcggatgtc aatcggacaa aaccaacgat gctgttaatg
      481 cgatgcttga tttgatcagg aacatgcccc aaaaaccgga gcgttggaca tcattacaat
      541 caagcctgat acaatcggcc cagtcggaaa gacccggatt taaatccatc attgaaacca
      601 ttgaaggatg gaaatacaga ggatacaatc aggatcctaa cattgaaatg attgccaact
      661 acaaaacaat ggaatttgaa aatgtagttt ccttctggaa aaacgagatt agtcaaaaac
      721 caattaccat tacacttgta ggcaataccg gagcattcga tatgaaagcc ctggaaaaaa
      781 tcggaaagat cacgcttgta aaaaaagaac aaatgatgaa gaagtgattt ctgccttctt
      841 caacctgaat ttaaattcaa cataaacaca gacgctcgac aattaagtgg aaaaaaaagc
      901 tcgcatcatt gcctctttgg atcatttttt ggatgatcaa cagcaaaaac ttctggccga
      961 tgcactgcag gaaactccac cggtaagtat tcgtattcat ccgcttaaaa agttttttcc
     1021 ggaaaacgaa aaacaggttt cctggtgtag tttaggttac tacctttcag aacgtcctgt
     1081 ttttacccgc gatcctcttt ttcatgccgg atgttattat ccgcaggagg ccagttctat
     1141 gtttctgtat catgtattga gcgaaatttt acctgaaaac cctgtccgca tactcgatct
     1201 ctgcgctgct cccggaggca aaactacact tgccgcgaca ctactcagag aaaatgattt
     1261 actcattgcc aatgaaattg aacgaccgcg ctgctctgta cttcgagaaa acgttttgaa
     1321 atacggtgat ccgcgggtgt atgtcaccaa taacgcaccg gaaaactttt ctgcgctgga
     1381 aggatttttt gatatcatca ttgtcgatgc accctgttca ggagaaggta tgatacgaaa
     1441 agacccacgc gctctcgagg aatggtcgga aacaaatgtc cgcacctgca cagttcgtca
     1501 gcacgatatc ctccaaaaaa tatggccggc cctaaaagaa ggaggcgttc tcgtatacag
     1561 cacttgcacg tacaataccg atgagaacga aaaacaaatt gaaaactgga tcagtaatca
     1621 ttccgctgaa aatattgaac taaatgttcc tgacgaatgg aacatcacaa aaacaaaagc
     1681 aggtgatgtt tcctgttacc gtttttttcc tcacaaaacc cgaggtgaag gtttcttttt
     1741 atcggcactg cgaaaaacgg aaacctgcaa cgaccactac atccgtaaaa aggatatggt
     1801 gtctcctaca cgcgaaaaag aaattgcaga atgggtgcta ccacaatggc aaaaattcct
     1861 gatccgtaat ggcgactcca tcgtactgca aacccacagt actgaaatcg tccgactaaa
     1921 aaaacatttg cgttttgcac ttgaatccta cccggtggcc gtcaaaaaag gaaaggatat
     1981 cattcctcaa cccgaatttg caatgaatat tgccctgaat cccgatgctt ttcaatcgct
     2041 cgaacttggt caggatgaag cattgcgatt tctaaaaacg gaagacatca gcacacaagg
     2101 caaacccgga tggaacctta ttacctataa gcagacgcct ctgggatgga tcaaacaatt
     2161 acagcaacgc tccaataatt attttccgaa agaatggaaa ataagaatgg aactgtaacg
     2221 acaattatta ccctcagata gaaaattgcc ccagcatcac tgcagggttc tccatcagtt
     2281 gtttaaaatg attcaggaac gcagctcctg ttgcgccgtc aacaacgcgg tgatcgcagg
     2341 ataaggtcac tttcatgata ttaccgggaa caactgcacc attttttaca actggaacct
     2401 gttttattcc gccaacggca agaatacagg catcaggcgg attgatgatt gcggtgaatt
     2461 cttcgatacc aaacattcca agattggaga tggtgaacgt attgccttcc caatcggatg
     2521 gctgcaattt tttatctttt gctttttgtg cataatcgcg cacttcagca gagatctgcg
     2581 ataatggttt agtatcagca aaacgaacta caggaacaag taatccgtct tccaccgcaa
     2641 cagcaacccc catgtgtaca tgttgattgt aacggatgcg atcgcccaac cagctggagt
     2701 taacagcagg atgttttttc aatgccaaag cagctgcttt aatgaccata tcattgaagc
     2761 tgactttaac tccggaaata tcagcaatgg ctttacgtgc atcgattgca cgatccatat
     2821 caatttcgag tgtgaggtaa aagtgaggtg ctgtaaattt gctttccgcc aaacgacgtg
     2881 caatggtttt gcgcatttgc gaaacagcaa cttcagtata actttcaacg ccggtggaca
     2941 tcattcccat cccaccgaaa ttttcaatat cgcgttttac gattcttccg ccgtcgccac
     3001 taccctgcag gagatttgga tttaacccct tctcttcggc aagacgtttt gccaatggag
     3061 aaatttttgt gcgaccattt gcattggcaa caggtgcgct ggtcttaact gttttttgca
     3121 ccggagcaga aacagtctta ggtgcttcag cagcaatttt aacgggttcc tctttcgcta
     3181 caggaagttc agcttttact tctgcctgtg gagcaactgc ttttttcttt tcctcttcga
     3241 gaagtgcaga aatatcttca ccctgctttc caagaattgc taaaatggaa tctacaggtg
     3301 ctccctttcc cttttctact ccaatgtgta acaagacacc attctggtac gattcaaatt
     3361 ccatcgtagc cttgtcggtt tcaatatcag caagcaattc gcccgattta actgcatcgc
     3421 ctactttttt atgccactgc gcaaccacac cttctgtcat ggtatcgctg agtttgggca
     3481 tgcgtatgat ttctgccatg gtagtagaaa taattaatat tcgataatgt aagggtagtt
     3541 gggttctgcg tacacatcat tgtacagctc agacggatcc ggtaatggac tctcctctgc
     3601 aaacttcacg ctctcttcaa caatcgcgtc aaccttctgc tcgatcgctt caatttcttt
     3661 ttcgcttagg tatttattcg cacggatcgt tgccaatact tgttcaatgg gatcctgacg
     3721 tttgtattct tccacttctt ccttcgtacg gtatttctga ggatcgctca tcgaatgtcc
     3781 tttgtagcga taggtcatga tatttaacaa ggtaggacca tcgcctctac gtgcacgttc
     3841 gcatgcttct tcaatggcaa ggtgaacggc ttcgcaactc attccatcta cattgaaaga
     3901 tggcatttcg tacgatgcac ccaatttgga aagatcagtt acattggttg tgcgttcaac
     3961 cgatgttccc attgcgtaat tgttgttctc tacaataaag atcacaggca atttccacaa
     4021 catggccata ttgaatgttt catgcaatgc accttgacga acagctccgt cgcccatgga
     4081 tgcaaaacat acgttatctg tgccaaggta tttttctgca aatgcaattc ctgctcccat
     4141 tggaatctgt gctcccacta ttccgtgtcc gcccatcagg ttggattcct tatggaacat
     4201 gtgcatggat ccgccttttc ctttcgacat tcccgtgctg cgaccgtaca attcggccat
     4261 catgtatttg ggatgaacac ccaacgcaat aggatgcgcg tgatcgcggt aggcagtaat
     4321 gtgtttatca ccgggacgac aagctgatgc tgttccagca accacagcct cctggccaat
     4381 atagaggtga caaaaacctc cgaacttctg ttgcaggtaa agctgtccgg ctttttcctc
     4441 gatttttcgc atgacaagca tgtcttcgta ccatttcacg tacgtctcct tggagaattt
     4501 cactttcttg tccgctgtcg ccaccagttt accgggggtc gacttcttcg cttttgtctc
     4561 tgaagccatg gaatggattt tggatcgggc aaatataatg ataaacgcct actgtgcagg
     4621 cgacttttta agcttggttt cgttgaaaac aagaggcagc agactgagca cactatcaat
     4681 cacaagcact ttccccgaac tccccgaaag tagcaaacga atgggttggt cctgcctgat
     4741 ctcgtattcc gacatgcttt ggcggcatgc accacaaggc gcaacaggct catccacacg
     4801 gaaatcctca gaaatagcaa caatcgccac cttctctata gtcttacccg gaaactgtgc
     4861 ccctgcagcg aaaaaggcaa cacgctcagc acaaagtccc gatgggtaag caacattctc
     4921 ctggttactt ccaattacaa tgcgcccacc actcaatcgc aaggcagcac caacatggaa
     4981 cctggaatag ggcgcatagg ccatctcggc agcttcaacc gctttggcaa tcaactcctg
     5041 ctcatcttgg gccaattcat ccctcgaatc aaatgactca tactgaaatt caaccttttg
     5101 aaccctggac gacataatga attaagtgtt tacgtaactt taagttcatg ttaggagcaa
     5161 ttacagaccc taacagggaa taaagatact acttagctaa tcatgattgc acctgaaaga
     5221 tcatttatca gcttcaacct tattggagga tgcagcagat tcattttgtt attcgcagca
     5281 ctttgcattt ccccgcttta cacacaggca caaaatgccg gcgatctgtt ttttggtgcg
     5341 gctactgtgc atgagatccg tttaaccttt caccagaatc aatattggga ttccttaaca
     5401 gcaggatatg ccaatgatgt gtacataaaa gcagatgtag agatagacgg atcaatgctg
     5461 gccaacagcg gagcgaaatt caaaggcaat tcttcttaca acaacccgag caataaaaaa
     5521 tcattcaaac tcgatttcaa cgaatatata cccggacaaa aatacaatgg tctgaaaaaa
     5581 ctgaacctca acaactgttt caaagacccc acatttctgc gggagaaatt gatgctcgac
     5641 tttctgggac accgcggcgt tcccgctcct cgttgcacct atgcagaatt gtatattaat
     5701 aatgtacgat ggggattata tacagttgtt gaagaaatcg ataaaacatt tttaaaacgt
     5761 gtcttcaacg aggataaagg caacaatttc aaaggagatc catccggcga tttaaaatgg
     5821 ttaggccctg cagcttccaa ttattatccc aaatacgaac tgaagaccaa cgaaacgcta
     5881 aacaactgga acgatctgat tctgttcatc aataaactga acatgacatc cacagctgtt
     5941 ttacccgata cattggccaa ttattttaac ctcgaagaat attacaccac ctgggcatca
     6001 catattcttt tttcaaatct tgattcctat ttaggcagtg gccataatta ttacgtatac
     6061 cacgattcac tcagcaataa attcaggttc attacatggg atgtaaacga agcatttgga
     6121 aatttcaata tgggaatgaa tgtttcgcaa attgaatcac tatctatttt cttcacgccc
     6181 aatcccgcag gaaaccgtcc gctgcatgaa cgtttattac agaacaattc cgtaaaacaa
     6241 cgtttggcag atgaattatg tgcgatggtt aattacgatt tcagcatttg gacactggaa
     6301 aataaaattg attctctggt taccgttatt cgtccctatg tatacgccga taatctgaaa
     6361 tttttcagca atcagaattt tgaagataac attgatcaaa ccatcaccgt gagcggtgtt
     6421 cccggtggaa atacacttcc gggtattaaa tcatttctaa tcaaccgcag aaattcggtg
     6481 gccactcagc ttgcggcatg gtcctgcact gttggagtgg aagatgctaa cccgacagaa
     6541 ttgaccgctt tcccaaatcc cggcaatggt atgctgcaga tacttggtcc ggaaggaagc
     6601 agttgggatt attcggtatt cgacttaaat ggaaaaaaaa tgtcgggcgg acaattcctg
     6661 ctccccggaa gaatcaatac tgaaagttgg gattccaacg gacttttcct gattcgtttc
     6721 aataatcagc agacaaatag ctcatatgtc gtgaaatttt taaaacttta acaaaaccca
     6781 ttccaatgaa aagaatcctt tttccatcgt taatccttct gagcattcta tttacctctt
     6841 gtaaaaaaga agaaggtgtt gggggcatgg caaccattaa ggggaaaata tggatgaaag
     6901 aatacaccgg tggagttttg attgaccaat atttcgctcc cgatcacgat gtatttatta
     6961 tttacggcga agggaatacc tattacgacg ataaaatctc aaccagttac gatggttctt
     7021 acgaatttcg atacctacgc ccgggtcgtt acaagatttt cacctatacc aaacatgaga
     7081 tcacggggaa tttaagtccc gaaatccgct ctgtagaaat cagcggaaaa aaagatgtta
     7141 ttgttttgga tgatattacc gtaaaggatt gaccctctca tcttccatta tcgaacagtt
     7201 tgatgctatc agtcttgatg agatggatag tgtaaaactc atgaaccgaa ccgataccaa
     7261 gttcgcattc aatagtgagc gtctggatga aattctgagt ctttgcaaaa acgactaccg
     7321 ggttctcgca ataaacggaa agcgatcggc atcctacatt accctctatt tcgatacacc
     7381 cgatcattca ctgtatcttg ctcaccataa tggaaaacca tatcggtaca agatcaggat
     7441 cagaaactat gtggaatccg gattgtttta tctcgaaata aaacaaaaga taaagggacg
     7501 caccgtaaaa tcgcgcatca aactaaaagg gtttgaagaa gaattatccg atcggtcaaa
     7561 agaattcatt gaaaaaacga tcggtaaaaa actggaactg cgtcctgcct tatggaacaa
     7621 tttccgtcgt attaccctcg tgcactgcgt tgccaaagag agagtcacaa tcgatacctc
     7681 acttaccttt tcactacaaa acacctcgcg cgaaacttct gaacttgcca ttgcggaagt
     7741 aaagcaggaa aacagaaaca ggaattccgg catcattcaa atactccgaa aactgggcat
     7801 acgtgaatcc ggaataagca aatattgcgt tggctccgcg ctgattaaac cacaattgaa
     7861 atacaatacc tttaaagaaa agattctgtt tattgaccga ctaaataaag tagcctgaca
     7921 ccatgctgat tgattttttt aatgatttcc tgttccagct tcagacagac gatctgagca
     7981 ctattgattt acccacagcc aaactcaaag cgaaagccgt gaaagattca ttattgggca
     8041 tgcccattta cgatgatgat ttctggaaaa tgatcattcg ttttgccatt aaccttaccg
     8101 ttttagtggt aatgatacgt ctggtttact accccacatc gaaacgaaaa gattatctgt
     8161 tcacttacat gatgattgga ttcatttcgt ttttcatttg tatcgctctt aaaaataaag
     8221 atattgatac cggaatggcc ctggggcttt tcgcgatatt ctccattata cgttaccgaa
     8281 ccgatgccat tccaattcgg gaaatgacct atttatttgt agtgatcgga atctcggtta
     8341 ccaatgcatt aacaggaaaa gacagtagtc ttgttggact gctattcatc aaccttacat
     8401 tattgatcct ggtgtatggt ttcgagcgca tctggatgtt aaaacatgaa agctcgcaat
     8461 tggtgatcta tgaaaaaata gaattgatca aaccacaaaa acatcaggaa ctcattcagg
     8521 atcttgaaga ccgtacagga ctaaaaatta acagagtcag catcggtaag attgatttta
     8581 tgcgtgatat cgcgcaaatc atgatctact attatgagag cgatcagcgc aacatcagca
     8641 attattcagc atcatccgga agcgacgacg gtgatgattg atgattgcac gaagagagaa
     8701 aagcaagacg actaaacacc gatcagaaga accacagcag aagcagttaa cccttcttcc
     8761 ttaccgacat aacccaactt ttcgttggtg gtagctttga tggaaacatc atccatttca
     8821 acttcaagaa tagaggcaat gctttcacgc atggcatcga tatgcggttt tattttgggt
     8881 ttttcaaggc aaagcatcga atcgatatta tttaccctat agcctttttc ccttacaagc
     8941 gcgtaggtgc gtttcaataa aattttcgaa tctattcctt tgaactcatt ggaagtatcc
     9001 ggaaaatgat gtc
//
LOCUS       DAOMYG010000065         8925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2597_length_8925_cov_9.786020, whole genome shotgun sequence.
ACCESSION   DAOMYG010000065 DAOMYG010000000
VERSION     DAOMYG010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8925
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2597_length_8925_cov_9.786020"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PK610_03375"
     CDS             complement(<1..248)
                     /locus_tag="PK610_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ34996.1"
                     /translation="MRLLNTTLFLLLLGNTTFSQEHDTLLTETARYIAGIPCETEYFK
                     TLQQKSFYKAHSDFCAKSWEELSDSTLNPMVKWATEKN"
     gene            403..1008
                     /locus_tag="PK610_03380"
     CDS             403..1008
                     /locus_tag="PK610_03380"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRJ34997.1"
                     /translation="MSEIQQLIALLPATSKWSYDAYYAFSEKLVEEGKTTGPDQSPAM
                     IEYSRLNLHRMKKWNKVGDLLPGCANELKNVKPQTWYVLSEPWCGDAAQSLPFISKMA
                     GNSEGKIELVILLRDENPSIMDRYLTNGGRSIPKLIALSETGEELFTWGPRPEKAQEL
                     VVTLKNDASVSADEMKERLHKWYAENKGKDVQQEILNLIVS"
     gene            complement(1009..2043)
                     /locus_tag="PK610_03385"
     CDS             complement(1009..2043)
                     /locus_tag="PK610_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRJ34998.1"
                     /translation="MKQLTTLAILLSAFSLGAQDIHFSQYMESPMNLSPALAGTDASS
                     ARANLNYRNQWGSFDKAYSTMGASFDIGLMRDPNKSAFLGAGLSILNDKAGTGGLNRM
                     SVAGNLSSVIEVGNKSYLSVGIQGSFNQRSISMDAFKWDAQFQNLSYNSSLASGETGG
                     SLSKTFFDLGAGLAFVKHGNSSNLSNADDFSMIAGLAAYHINRPNQAFQGSDKMNMRY
                     NGFLQMSFGIGGSNMSIQPQLAYWRQGTLQEINTGMMFRYVLKPESQHTGFERGIALS
                     IGGFYRVKDALYPMMLLEYGDFALGMSYDVNVSSLTPYSQSRGGFEISLRYRDLNGLV
                     FGKPGQRRML"
     gene            complement(2047..5997)
                     /locus_tag="PK610_03390"
     CDS             complement(2047..5997)
                     /locus_tag="PK610_03390"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ34999.1"
                     /translation="MKTFSRSFASIERFSFGTLILFLGFQLSFSEANAQRGIKGNVTI
                     STANTIVNEYTRLTANAVSGSTSITVLDNTLDSVAARFTTPLAAGDLIFIYQAQGATI
                     NGTLSGGIGIPNDATWGGVTNYGSSGNYELAEVASVSGGTIINLTCGLRNDYTTAGRT
                     QVIRVPRYENLIINAGSGIIPHAWNGTIGGIVVVEVNTATTINGTINANGRGFRGGAL
                     DPNSQFNPNFTTILDIDGAIKGESIAGYQTDLTPNGGMYCMAAPANGGGGGNAHNAGG
                     GGGANGGDILLWDGRGNPSITTASWANAWNLEAPGFSTHTSSGGGRGGYSWSNSNQNA
                     TTLAPGSAAWGGDNRKIYGGYGGRPLDYSTGKIFFGGGGGAGDQNENFGGAGGNGGGI
                     IFLYSHGTVGGTGTITANGNNGNSTLIPAPGFGQCTGKDGAGGAGGGGCVIINSNGSI
                     AGISIVANGGNGGNQNLSCNTFTPAGDAYGTGAGGGGGYIAISNGAISRTTNGGDNGT
                     CNAPAMTEFPPNGATRGGAGVNNASIANHFLTVNPANINICASATATLTATLTGALPS
                     GASINWYDSQFGNTIIGTGLTFTTPVLTSTTTYYVGICPGFYRIPVTVTVGSTPVIDI
                     SNMLITNATCGNNNGAINNIIVTGATSYTWNGVNSATPDLSNAAPGSYTLIASGGGSC
                     TATAGPFTISTGGGPVINTASMVITPTSCGINNGSITGITATGTAPLSYTWNGAANND
                     ADTNQLAAGSYTLLVTDGLGCSAASGPHAIAASTAMSFNSSGIVITPATCGSTNGSIT
                     GIILNGGSAPISLQWNGTPAATTDISGIGSGNYTITATDNAGCTASSGPFTVTSTGGA
                     SINDANIVIVHTSCGNNNGSISGILVSGGTSPYTFAYNATNNPTADISNLTAGSYTFS
                     VTDDQGCVTASGPYVINPSSSPTVNVTGSNVSCFGANDGTATASGSGGTGILNYQWTS
                     GPAGAVFAGTGAGTFSVTVTDAAGCTATGSVTLTEPAEIIAAISGTNSICNGQNTTLT
                     ASGGTSYSWSSGDNTAATTVTPTITTTYTVTVTVGSCSTTSSVTVTVNDVPVASISGT
                     TSVCPGQSTTLTASGGSTYLWSSGETTPSITISPTANGPLSVTATNGCGSDNETVNLI
                     VFPAASASAGADVTIALGGSTTLSASAGTSYSWLPSTGLNQTNTQSVIASPGSTTTYI
                     VTITDSNGCTASDDVIVSIDMQMVVFVPDAFSPNGDGNNDQLFVRGAGIREFTFRVYD
                     RWGEKVFESTDLQSGWDGNYKGSLLNSGVFVYTLEGEFISGEKFDQKGNVTLFR"
     gene            complement(5963..7261)
                     /locus_tag="PK610_03395"
     CDS             complement(5963..7261)
                     /locus_tag="PK610_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35000.1"
                     /translation="MQKISSYLEKFQLVITAGLFLFLTFNMHSRAPIFSYHHVLWADA
                     AGYYSYLPATFEFGWKAEHFPDSADILTGQGFRLNSETNSIETKYTYGVALLQLPFYL
                     TGRLIAKISGSNENGFGVIGHRMIMLAGVFYALAALYFLKRILKKMYGAFSASMAVLF
                     LFSSTTLFYYGIDKAGFSHIYLFFLFTVLINMVDRLIYSPDFFRSLIFFAILLLCFLI
                     RPTSVFAFPLIFLTGVKSKADLFELLHALRKNRKHYLLPACLATLVLIPQFTYWYFIS
                     GNIFWYSYGDEGFSNLNNPHLLELWFSPNNGLFVYTPIVAVLLIISLLKFNRGIDSIS
                     FLVVFLFISWMFASWHDWRFGCSFGSRSFTEYLVFLAFPLAGLLNHLKEGRYHKLLLF
                     CCLMIGYIAFVNINTIYTYDDCFYGDTWDYENFFKIIRFY"
     gene            7314..>8925
                     /locus_tag="PK610_03400"
     CDS             7314..>8925
                     /locus_tag="PK610_03400"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRJ35001.1"
                     /translation="MNRKVRSLRLCLLFLLLPLFSAAQFDYPKTRKSADSEIFHGTTV
                     ADPYRWLECDTCAEVRSWVIEQNKVTQKWFAAITYRNEIRKRLAKIWSFSSMTPPSKK
                     ADKFFIYKNNGTQNHGVLYWFTQKDSTHKVLLDPNTFSVDGTTSLNSAVPDSKAKYIA
                     YSISHKGSDWSEIKIKEIQSGVDLKDHLKWVKFSGISWYKNGFFYSRYDAPKGAEYSV
                     RNEYHKVYYHTIGTNQSADVLIYSENDKPLRNFGVSLIPNSTWLALSATEGTSGNSVR
                     VADAKNWKPGGKLEWKVVADGFSDDYSPVGFVKGKLLFLTNASAPNRKLIAVNPNNLL
                     PGSQVVVIPERESVLESVSIANGKLVVKYLENVSSKLQVFSLEGNYERDIELPLIGMV
                     SGVSGSMNEPVIYYSMVSYTFPSRIYKVDLRNYQTSLYFKPEIDFKSENFETKQVWYT
                     SKDGTKVPMFITHKKGLKYDGNNPLFLFGYGGFNISYTPEFRVDRSVFLENNGIYCVA
                     NIRGGGEFGQNWHKAGTKLQKQNVFNDFI"
BASE COUNT         2572 a         1904 c         1875 g         2574 t
ORIGIN      
        1 atgttttttt ctgttgccca tttcaccatc ggattcaatg ttgaatccga taattcttcc
       61 cagctttttg cgcagaagtc ggaatgtgct ttataaaatg atttttgctg tagggtttta
      121 aaatactccg tctcacaggg aatacctgca atatatcggg ctgtttcagt caacagggtg
      181 tcgtgttcct gagaaaaagt agtgttgcct aatagcagta aaaaaagcgt tgtattaagt
      241 aatcgcataa tcattgaaat ggggtgtaaa agtacaaaat catgcaaatg ggtatgcccc
      301 cttcaacctg acagtgcagg gttccggagg catccgcaaa accaataagc ataattgaca
      361 tatatcacgt ttcgtgccac aattgctttc tatctttgtg ctatgagtga gatccaacaa
      421 ttaattgcgt tgcttcccgc cacttcaaag tggtcgtacg atgcctatta tgcattttca
      481 gaaaaactgg tggaagaagg aaaaactaca ggccctgacc agagtcctgc catgattgag
      541 tattcccgtc tcaatttgca ccgaatgaaa aaatggaata aagtagggga cttattacct
      601 ggttgtgcca atgagctgaa gaatgtaaaa ccacaaacct ggtatgttct ttctgagcca
      661 tggtgcggcg acgctgctca gtctttgcct tttatttcta aaatggcagg aaattctgaa
      721 ggcaaaattg aattggtgat cttgctaaga gatgaaaacc cgtccattat ggatcggtac
      781 ctcaccaatg gtggaagatc tattccaaaa ttgatcgcat tgagtgaaac aggcgaagaa
      841 ttatttacat ggggaccgcg tcctgaaaaa gcacaggaac tcgttgtgac cctaaaaaat
      901 gatgcttcgg tttcggcaga tgaaatgaaa gagcgattgc acaaatggta tgcagaaaac
      961 aaaggcaagg atgttcagca ggaaattctg aatctgatcg tttcctgatt acagcattct
     1021 tcgctgaccg ggtttcccaa ataccaatcc gtttaaatca cggtacctaa gagaaatttc
     1081 aaatcctcct cttgattgag agtaaggggt aagagaagaa acattaacat catatgacat
     1141 tcccaatgcg aaatcgccgt actcgagcaa catcattgga tacaatgcgt cttttacacg
     1201 gtaaaaacca ccaatggaga gggctattcc gcgttcaaat cctgtatgtt gcgattccgg
     1261 ttttaataca taacggaaca tcattccggt attgatttcc tgcaaggttc cctgacgcca
     1321 gtaggccagt tgtggttgaa tcgacatgtt gcttcctcct ataccaaaac tcatctgcag
     1381 aaatccattg taccgcatgt tcattttgtc gcttccctga aacgcttggt tcgggcgatt
     1441 aatgtggtag gctgctaatc cggcaatcat ggaaaaatca tcagcgttcg acaaattact
     1501 ggagttgcca tgttttacaa atgcgagtcc cgcacccaaa tcaaaaaacg ttttgctcag
     1561 cgaaccacct gtctctccac ttgcgaggga agaattgtaa gataagtttt gaaattgtgc
     1621 atcccattta aatgcatcca tagaaatact tctctggttg aatgatcctt gaattccaac
     1681 acttaaataa cttttatttc caacttcaat tacagatgac aagtttcctg ccacactcat
     1741 tctgttaagt cctcctgtac cggctttgtc attaagaatg gataatcccg cacccagaaa
     1801 tgcactttta ttgggatcgc gcatcagtcc gatatcaaag ctggcaccca ttgtactata
     1861 ggctttgtcg aagcttcccc actggtttct gtaattcaga ttggcacgtg cagaactcgc
     1921 gtctgtacct gcaagtgccg gtgaaagatt cattggactt tccatatatt gtgaaaagtg
     1981 aatatcctgc gcaccaagtg agaatgcaga taataatatt gctaaggtag ttaactgttt
     2041 catagattaa cggaataatg taacgttacc tttctggtca aacttttctc ctgaaataaa
     2101 ttctccttca agagtataaa caaacacacc tgaattcaac aaacttcctt tgtaatttcc
     2161 atcccaaccg gattgtaggt cggtgctttc aaatactttt tctccccaac gatcgtacac
     2221 gcggaatgtg aattctctga ttcccgctcc ccttacaaat aattgatcat tgtttccatc
     2281 gccatttggt gaaaatgcat caggtacaaa tacgaccatc tgcatatcaa tcgaaacaat
     2341 tacatcatca ctggctgtac atccatttga atcggtaata gtaacaatgt aagtggtggt
     2401 tgatccagga ctggcaatta cgctttgcgt gtttgtttga tttaatccgg tggaaggtaa
     2461 ccatgaataa cttgttcctg cacttgcaga aagtgttgtt gatcccccga gcgcaattgt
     2521 aacatccgcg ccggcagaag cagatgccgc tggaaatact atgagattta ctgtttcatt
     2581 atcactgcca catccgtttg ttgcggtaac actcaacgga ccgtttgcag tgggagaaat
     2641 tgtaatggaa ggagttgttt ctccactgct ccaaagatag gtacttccgc ccgaagcagt
     2701 aagtgttgta ctctgaccgg gacatactga tgttgttccg cttatacttg caaccggcac
     2761 atcgttaacg gtaaccgtaa cagatgatgt agtactgcat gagccaactg taacggtaac
     2821 cgtataggta gtggtaattg ttggagtaac ggtagttgcg gcagtattat ctccgctcga
     2881 ccaggaataa cttgttcctc cgcttgccgt taatgtggtg ttttgtccgt tacaaatgct
     2941 gttcgttcca ctaatagcag caatgatttc tgcaggctca gtaagggtaa cagaaccggt
     3001 tgctgtacat cctgcggcat ctgttacagt tacactaaaa gttcctgctc cggttcctgc
     3061 aaatacagca ccggcaggac ctgatgtcca ttggtaattt aaaattcctg ttccaccact
     3121 tccactggca gttgctgttc cgtcgtttgc accaaaacac gaaacgttag aacctgttac
     3181 attaactgtt ggagaggatg aaggattaat gacatagggt ccgctggcag ttacgcaacc
     3241 ttggtcatcg gtgaccgaaa aggtatacga tcctgcagtt aaatttgaaa tatctgcagt
     3301 tggattattt gttgcattgt aagcaaaggt atacggagaa gttccgccac taacaagaat
     3361 tccggaaatg cttccattgt tgtttccaca ggaagtatga acgataacaa tattggcatc
     3421 gttaatactt gcaccacctg ttgaagttac tgtaaatgga ccggagcttg cagtacaccc
     3481 tgcattatca gttgctgtga tggtatagtt cccactgcca attccactta tatcggttgt
     3541 tgccgctgga gtgccattcc attgtaaact tattggagct gatcctccat ttagaattat
     3601 tcctgttatg cttccattgg ttgatccgca tgttgcaggt gtaataacta tacccgaaga
     3661 attaaaactc attgctgttg atgctgcaat cgcatgaggt ccgcttgcag cgctgcaacc
     3721 taatccatct gttaccagca aggtgtaact tccggcagca agctgattgg tgtctgcatc
     3781 attgttggca gctccattcc acgtatacga aagcggtgca gtgcctgttg ctgttattcc
     3841 tgttatgctg ccgttgttta ttccgcatga ggttggtgta atcaccatgg acgctgtgtt
     3901 aattactggt ccgccaccag ttgaaatggt aaatggaccg gcagttgctg tacagcttcc
     3961 gccaccggaa gcaataagtg tgtaagagcc gggtgctgca ttgctcagat ccggtgttgc
     4021 ggagtttact ccattccagg tgtaggatgt cgctccggtg acaatgatat tattaattgc
     4081 accattgtta tttccacaag tggcgttggt gatcaacata ttgctgatat caattacagg
     4141 tgttgatcca actgttactg ttacaggtat tctgtaaaat cccggacaaa ttccaacata
     4201 ataggtagta gttgaggtga gaaccggagt tgtaaatgtt aatcctgttc caataatagt
     4261 atttccaaat tgagaatcgt accagttgat acttgcaccg gaaggtaaag ctcctgttaa
     4321 ggttgcagtg agtgttgcag ttgcagatgc gcagatgttg atattagcag gatttacagt
     4381 gaggaaatga ttggcaatac ttgcattgtt tacaccggcg ccaccacgtg ttgctccatt
     4441 aggtgggaat tctgtcatag caggtgcgtt acaggtacca ttatcaccgc catttgtagt
     4501 acgggatata gcgccatttg aaatggcaat atatccgccg cctccaccag cgccggtacc
     4561 gtaggcatcg cctgctggag taaatgtgtt acaggaaaga ttctgattcc cgccatttcc
     4621 tccatttgca acaatactga tacctgcaat tgaaccattg gaattgataa taacacagcc
     4681 accgccgcct gcaccaccag cgccatcttt tcctgtgcac tgaccaaatc cgggtgcagg
     4741 aataagcgtg ctgtttccgt tatttccgtt tgcagtgatt gttcctgttc caccaaccgt
     4801 accgtgtgag tagagaaaaa taattcctcc accgttaccg cctgcgcctc caaaattttc
     4861 attctgatcg cctgcaccac ctccaccacc gaaaaatatt ttccctgtgc tgtagtctaa
     4921 tggacggccg ccataaccac cgtaaatttt tctgttatct ccaccccatg ccgccgaacc
     4981 cggagcaagc gttgttgcat tctggttgct gttcgaccag gaatatcctc ctcttcctcc
     5041 acctgacgat gtatgtgttg aaaatccggg tgcttcaaga ttccatgcat ttgcccagct
     5101 ggcagtagtt attgaaggat tacctcttcc gtcccacaat agaatatcac caccgttagc
     5161 accgcctccg ccacctgcgt tgtgcgcgtt tccgcctcca ccaccattgg caggggctgc
     5221 catgcaatac attcctccat tgggagtgag atctgtttga taacctgcaa tactttcgcc
     5281 tttgatggct ccgtcaatat caagtatagt tgtgaagttc ggattgaatt gcgaattcgg
     5341 atcaagcgct cctcctctaa aacctcttcc attggcattg atagttccgt ttattgttgt
     5401 tgcagtattt acttcaacaa caacaattcc tccaatggtt ccgttccatg catgcggaat
     5461 tattcccgat cctgcattga tgatcagatt ttcataacga ggaacgcgaa tgacctgggt
     5521 tcttcctgca gtggtataat cattccgcaa gccgcaagta agatttataa ttgttcctcc
     5581 actcacgctt gctacttctg ccaattcata atttcctgat gacccataat tggtaacccc
     5641 tccccaggtg gcatcattcg gaattccaat tcctccactt aatgtgccgt ttattgtcgc
     5701 cccctgtgcc tgataaataa aaattaaatc tcccgcagcc aggggtgtag taaatcgtgc
     5761 cgcaaccgaa tccagggtgt tatccaatac agtaatactt gtacttcctg aaacagcatt
     5821 tgcagtgagc cttgtatatt cgtttacaat cgtattcgca gtggaaatag ttacatttcc
     5881 tttaattccg cgttgagcat tggcttccga aaaggataac tgaaatccca aaaataaaat
     5941 cagcgtgcca aatgaaaaac gctcaataga agcgaatgat cttgaaaaag ttttcataat
     6001 cccaggtgtc tccataaaaa caatcgtcgt aagtgtaaat ggtgttgata tttacgaaag
     6061 caatataacc aatcataaga caacagaaaa gtaaaagctt gtgatatctg ccttctttga
     6121 gatgatttaa tagaccggct aatggaaagg caagaaatac aaggtattcg gtaaaacttc
     6181 tgcttccgaa agaacacccg aatctccagt cgtgccagga agcaaacatc catgaaatga
     6241 acagaaacac cactaaaaaa gaaattgaat caattcctct attaaacttt agcaacgaaa
     6301 ttatcagcag aacagcaaca attggtgtat acacaaacaa gccattattg ggactaaacc
     6361 ataattccaa taggtgggga ttgttcagat tactaaatcc ctcatcgcca tacgaatacc
     6421 aaaatatatt tccactaata aaataccaat aggtgaattg aggaattaaa accagtgttg
     6481 ccaagcatgc aggaagcaaa taatgttttc tgtttttcct taaagcatgg agtagttcga
     6541 acagatctgc tttcgatttt acccctgtaa gaaagataag gggaaatgca aaaactgatg
     6601 tgggtcgaat taaaaaacaa agaagaagaa tcgcgaaaaa aatcagggat cgaaaaaaat
     6661 caggcgaata aattaatcta tcaaccatgt tgatcagtac agtaaaaaga aagaacaaat
     6721 agatatggga aaaacctgct ttgtcaatcc cataataaaa taacgttgtg gatgaaaaaa
     6781 gaaagagcac agccattgaa gcagaaaatg caccatacat ttttttcaaa atccttttta
     6841 aaaaatagag cgctgccaag gcatagaata cgccggcaag cataatcatt cgatgaccaa
     6901 ttacaccaaa accattttcg ttagatccac tgattttggc aatcaatctt ccggtcaggt
     6961 aaaacgggag ttgaagtaat gccactccgt aagtatattt tgtttcgatc gaatttgttt
     7021 cagaattcag ccggaatcct tgccctgtga gaatatctgc agaatcagga aagtgttctg
     7081 ccttccaccc aaattcaaat gtggccggca ggtatgaata atatcccgca gcatctgccc
     7141 ataacacatg gtggtaggag aatatcggtg cacgggaatg catattaaag gtcaggaata
     7201 aaaacaatcc tgccgttatt acaagttgaa acttttcgag gtatgaggaa attttttgca
     7261 ctccttaatc gatccatttg tatctttccg caagttacta atctgattgt tatatgaatc
     7321 gaaaagtcag gtctttacga ctttgcttgc tttttctttt gttgccgctg ttttccgctg
     7381 cccaatttga ctatcccaag acgcgaaaat cagctgattc tgagattttt catggaacta
     7441 cagttgccga tccttacagg tggttggaat gtgacacatg cgctgaggtt cggtcctggg
     7501 tgatcgagca aaataaggtg acccaaaaat ggtttgcggc gataacttat cgaaatgaaa
     7561 tacgcaagcg acttgcaaag atctggagtt tttcatccat gacacctcct tcgaaaaagg
     7621 cggataagtt ttttatctat aagaataacg gcacgcaaaa tcatggtgtt ctgtattggt
     7681 ttactcagaa agattcaacg cataaagttt tgctcgaccc taatactttt tctgttgatg
     7741 gaacaacctc gcttaattcc gcagttccgg attccaaagc gaaatacatt gcttattcaa
     7801 tttctcataa aggttccgac tggagtgaaa tcaagattaa ggaaattcaa agcggagttg
     7861 atcttaaaga tcatttaaag tgggtgaagt tctccggaat ctcatggtat aaaaatggtt
     7921 ttttttattc gaggtatgat gccccaaaag gagcggagta ttctgtacga aatgaatacc
     7981 ataaggttta ttatcataca attggtacga atcagtcggc agatgtgctg atttattctg
     8041 aaaacgataa accacttcgg aattttggag ttagccttat tccgaattca acctggcttg
     8101 ctttgagtgc aactgaggga accagcggaa acagtgttcg ggttgctgac gcgaaaaact
     8161 ggaaacccgg aggaaaatta gaatggaaag ttgtagcaga tggtttctcg gacgattata
     8221 gtcctgttgg ttttgttaag gggaaattgc tgttccttac caatgcaagt gctccaaaca
     8281 gaaaattgat tgctgtaaat ccaaataatc ttttgcctgg ttcccaggta gtagtgatcc
     8341 ctgaaaggga atcggttctt gaaagcgtgt cgatcgccaa tggaaagttg gtggtgaagt
     8401 accttgagaa tgtcagctca aaacttcagg tcttctcgct ggaaggtaac tatgaacgtg
     8461 atatcgaatt gccactgatt ggtatggtta gcggggtttc cggttcaatg aacgagccgg
     8521 taatctatta cagtatggtt tcttatactt tcccctcacg catttataaa gtggatttgc
     8581 ggaattatca aacttcactt tattttaaac ctgaaatcga tttcaaatct gaaaattttg
     8641 aaaccaaaca agtgtggtac acctcaaaag atggaacgaa agtcccgatg tttatcactc
     8701 acaaaaaggg attaaaatac gatggaaaca atcccttgtt tttatttgga tatgggggct
     8761 ttaatatcag ttatactcct gagtttcggg tcgaccgttc tgtttttctg gagaacaatg
     8821 gcatttactg tgttgcgaat atccgcggag gtggagaatt tggtcagaac tggcataagg
     8881 ccggaacaaa actccaaaag caaaatgtat ttaatgattt tattt
//
LOCUS       DAOMYG010000066         8913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2605_length_8913_cov_9.029465, whole genome shotgun sequence.
ACCESSION   DAOMYG010000066 DAOMYG010000000
VERSION     DAOMYG010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8913
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2605_length_8913_cov_9.029465"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PK610_03405"
     CDS             complement(<1..610)
                     /locus_tag="PK610_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35002.1"
                     /translation="MKNTSIFQLLSMATLLLSFSPAIAHSDVIDPKPPMVVNKGQWDN
                     RALYRMTIGGGEAWLENGGITFHFRNQDDMEKLHLLHHNKIQASDYSGIRNHVYRQNF
                     IGANTNAQANGTEWANFHHNYFLGDDQSKWRGQVPVAYTVQYSSFYSGIDLRIGSKDG
                     YFKYDFIVAPGADPAQIKWSYSHIEPRLVNGKLIFSTHAGEVV"
     gene            772..2142
                     /locus_tag="PK610_03410"
     CDS             772..2142
                     /locus_tag="PK610_03410"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRJ35003.1"
                     /translation="MSVIPAFIESNKDRFINELLDLLRMPSVSADPKFKADVLKTAEA
                     IKARLIEAGADKVELCATPGNPIVYGEKIIDSKLPTVLVYGHYDVQPADPYELWTSPP
                     FEPVIKDGKIYARGACDDKGQVYMHVKAFETMMRTGTLACNIKFMIEGEEEVGSDNLG
                     PFVKANKDRLKADVILISDTAIIANDIPSIDTGLRGLSYLEVEVTGPNRDLHSGVYGG
                     AVANPINILAKMIASMHDENNHITIPGFYDKVNELSTADRAELNKAPFDLNEYKKDLA
                     IDQEHGEKGFTTIERTGIRPTLDVNGIWGGYIGEGAKTVLPSKAFAKISMRLVPNQDS
                     HEITKLFKDHFQRIAPKSVKVEVRPHHGGEPIVISTDSPGYKAASQAMTDTFGKKPVP
                     TRGGGSIPIVALFKKELGLDSILMGFGLDSDAIHSPNEHYGVFNYLKGIETIPLFFRY
                     FAEYNK"
     gene            2146..2607
                     /locus_tag="PK610_03415"
     CDS             2146..2607
                     /locus_tag="PK610_03415"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HRJ35004.1"
                     /translation="MRILLFSLTVLLSSCLSYEQVEFRNVERVNIKRDSNKEMVLNLG
                     VKLYNPNGYKIKVIKSQLDLTISGTSAGKAILEDKVILRKRKEEVYDINISIDRSAVT
                     KALMASGINLLFTGKVPVEVKGWIKGRVFIFGKKFDVAFKEQIDPKQFKMN"
     gene            2609..4615
                     /locus_tag="PK610_03420"
     CDS             2609..4615
                     /locus_tag="PK610_03420"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRJ35005.1"
                     /translation="MPNRLSTESSPYLRQHAENPVDWFPWGEEAFSKAKAEGKLVLIS
                     IGYSSCHWCHVMEHETFSDVAAAEVMNRYFICIKVDREERPDIDQVYMNAVQLMSGQG
                     GWPLNCFVMPDGRPVYGGTYFPRENWLKVLQHLNAIWKDEPEKVEDYAQRLTEGVQLS
                     DLAIPEEGSNTFSAAYADQVVQRWKNHFDSIEGGPNRAPKFPLPNNYEFLLRYAIYRN
                     DDSVMKHVRLTLDKIALGGIYDQLGGGITRYSTDMIWKVPHFEKMLYDNAQIISLYSE
                     AFRFTRDPLYLEIAERSIRFCIEEWSKPESGFYSALDADSEGEEGKYYVWKKDELQGL
                     LGEEYSWFSEYYNINYLGEWEHGNYILLRRERDDAFARKHKMDIDDFLQKKKKAHHLL
                     MTVRKKRISPLLDDKILCSWNALMITALCDAWKCSGKNEYRDKAISTAHFILREMLKG
                     DGGLYHCFHSGKAYVDGFLEDYAFCIEAFTRLFSITGDELWLAYADKWATYVLHYFSD
                     ENDILFYFTSSKSEALIARKTELSDNVIPASNSVMARSLHELGGIMGKIEYTHRANRM
                     LQTVYSHMEAYGAGYSNWGMLLLDLLEEPKEVVVCGEDADTMALRILQDVPPHVRVYV
                     CIAESDLPLFKNRFKTGQSLIYVCRNKSCNLPVQSPEEALEQLT"
     gene            4612..5514
                     /locus_tag="PK610_03425"
     CDS             4612..5514
                     /locus_tag="PK610_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35006.1"
                     /translation="MKASGIIFFFFLLSVHLPAQIIDNAGGTAFTDDNFFNSSFIRTN
                     KVQIIRGSVSTKREGEPMIQRSQFFHYEFDTKGRIILMMSTFASYSSNRDTTIIQFEY
                     NDKGLLATKRRNDNNGFYSYNYEYDSLGRLIKETYCRDENCGVDRYHFKLGQRFVISS
                     ETYSYHQYVKNQKVKKFYNNYGLQYQEEFSYYNELGYLTEEVLRLTLSGKESKIQYEY
                     DEKGRISKKNDISYVFGFNEISNTYTYDKLGNLDEQAIFHNGKQKTLRSMLYDKTTYL
                     MTAQVMKDFETGLIYVFKYSYEFF"
     gene            complement(5637..6581)
                     /locus_tag="PK610_03430"
     CDS             complement(5637..6581)
                     /locus_tag="PK610_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HRJ35007.1"
                     /translation="MKSLVFKSLALFVAGIFQIQANAQPAERRISRKEYVSQWVDEAQ
                     RQMLEYGVPASITLAQGILESGDGNSPLARYANNHFGIKCHDNWNGETFIQDDDRRDE
                     CFRKYNTAGESYRDHSLFLKNRSRYASLFTLDQTDYRGWAKGLKKAGYATNPQYADIL
                     IQIIEDNGLNHYDVVNNIAAKKPSVARQEEFNGINQIQHKVKVHDNHIKFIIAKDGDT
                     YYKISKEFELGLWQIYKYNDLSKKDVLQPGDIIYLQPKRNKAQVETHRVQEGDTMHNI
                     SQKYGIKLSKLYKLNNITPGTGEPEPGTLLHLKSRKKA"
     gene            6779..8269
                     /locus_tag="PK610_03435"
     CDS             6779..8269
                     /locus_tag="PK610_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35008.1"
                     /translation="MRIIVLIIMILGVSGSCLAQIKQVARLELELYEEGADHTDILGP
                     HGLIVHGRINIKGRGDLWSIRRFNTDLKLIDSTGFAISERMILTQSWLTDDFSALYFL
                     FTEKAKRTYYKLLRFDTPTGKMSVKEAELPKGTFFNKMSVVGNSIFLSGTLDKKTALF
                     LFDFEAGSFTNVNYIPLQKYSVIGMDVPAEEPMLMVTDAKGMGKGFSFRVSFFDEKGI
                     PTFKPQAVISQPDYYCRSASLTLLGGNEYILTGSYSQGKKSTANGVYFTRYENDQQIM
                     LKYHSFSGFKNFYEYLNPRQKERMEKKAEKKAKKGKEEFIEAYIASHPVFTFNGNYMY
                     VGEIYYPTYRTEVYYTTGPNGQMMRQTRRVFDGYQYTHAVVLAMDKDGNKVYDHCFPM
                     WSGFKPMTVRRFLRIVVGSDQADLLFGNGSTVKGMRITGNNIAELNYGEIETGNENDK
                     VRWTSMTQTSYWYDNYFISYGSQSIKNSDENGKKRRTVFFVTKMQF"
     gene            8521..>8913
                     /locus_tag="PK610_03440"
     CDS             8521..>8913
                     /locus_tag="PK610_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35009.1"
                     /translation="MIKQLFFLTCLFLIVFPGRSQMREVARVENKYRQGADYMFLNRE
                     NGIVLGGLVFAEDKKPMTYAFKHYNTDLKLTDSVVFEVPMGFALFGNRKIEDDIYFVF
                     STYNKTPVYIIARYRIPEKKVELKRGTLP"
BASE COUNT         2583 a         1655 c         2022 g         2653 t
ORIGIN      
        1 ccacaacttc tcctgcatgg gtggagaaaa tcagtttgcc gttcaccagc cttggttcga
       61 tatgggaata actccatttg atctgcgcag gatctgctcc cggagctaca atgaaatcgt
      121 atttgaaata accatccttg cttcctatgc gtaaatcaat tccggaatag aaactgctgt
      181 actgtactgt gtaggctaca gggacctgac ctctccattt gctttgatca tcacccaaaa
      241 aatagttgtg atgaaaattc gcccactctg ttccgttggc ctgtgcattg gtgttcgctc
      301 caatgaaatt ctgacggtaa acatggtttc tgatccctga ataatccgag gcctgaattt
      361 tattgtgatg cagcaaatgc aatttttcca tgtcatcctg attgcggaaa tggaaagtga
      421 tcccgccgtt ttcgagccag gcttctcctc cgcctatggt cattctgtag agcgcgcgat
      481 tgtcccactg acccttattc accaccatcg gaggttttgg atcaattaca tcgctgtggg
      541 caatggccgg actaaaactc agaaggagag ttgccatgga gagcaactgg aaaatggatg
      601 tgtttttcat gttaaaattt gaaaaagagt gatttaccac aaatatcgga acaaagacta
      661 agaaaggcct ataaaggttg catcttggtt tattccgaaa aatcagacgg caatgcctgc
      721 caaaaatgaa gcgcggggat aaagcgtata tttgcccgaa atatttctaa aatgagcgtc
      781 atacctgcct tcatcgagtc caacaaggac cgctttatca atgagttact cgatcttttg
      841 cggatgccga gtgttagtgc tgacccgaag ttcaaggcgg atgtgctaaa aacagctgaa
      901 gcgatcaaag cccgattgat tgaagctggt gcggataaag tagaactctg cgctacaccg
      961 ggaaatccaa ttgtgtacgg cgaaaaaata attgattcca aattgccaac cgtattggtt
     1021 tacggtcatt acgatgtgca acctgcagat ccctatgaac tttggacatc acctccattt
     1081 gagcctgtga tcaaagacgg aaaaatttac gcacgtggtg cctgcgatga taaaggtcag
     1141 gtgtacatgc acgtgaaagc gttcgaaacg atgatgcgca caggtacgct tgcctgcaat
     1201 atcaaattca tgattgaagg tgaagaggaa gtgggatcag ataatcttgg tccattcgta
     1261 aaagcaaata aagatcgtct gaaagcggat gtgattttaa tttccgatac ggcgatcatt
     1321 gccaatgata ttccttctat cgataccgga ttgcgcggat tgagttatct ggaagtggaa
     1381 gttaccggtc cgaaccgtga tcttcacagt ggtgtttacg gtggtgccgt tgcaaatcca
     1441 atcaatattc ttgcaaaaat gattgcgtcg atgcacgacg aaaacaatca cattaccatt
     1501 cccggttttt acgataaagt aaatgaactg agcactgcag atcgtgcaga actgaacaag
     1561 gcgcccttcg atctgaatga atacaaaaaa gatcttgcta ttgatcagga acatggtgaa
     1621 aaaggattta ccactatcga acgcaccggt attcgtccta cactcgacgt aaacggaatt
     1681 tggggtggtt acataggaga aggtgctaaa accgttcttc cttcaaaggc atttgcaaaa
     1741 atttcaatgc gtctggttcc caatcaggat tcgcatgaga tcaccaaatt attcaaagat
     1801 cattttcaac gtatcgcacc taagagtgtg aaagttgaag ttcgtccaca tcacggtggt
     1861 gagccaattg ttatctccac tgattctccg ggttataaag cggcgagtca ggccatgact
     1921 gatacttttg gtaagaaacc cgttccaacg cgcgggggtg gaagtattcc gattgttgct
     1981 ttgttcaaga aggaacttgg tcttgactcc attctgatgg gatttggact cgacagtgat
     2041 gctattcact cacccaatga acactatggc gtattcaact atttgaaagg aattgaaact
     2101 attcccttgt tcttccgtta ttttgcggaa tacaacaagt aaagaatgcg catactgctt
     2161 tttagtctta ctgttttact gagttcctgt ctttcctacg agcaggtcga attccgtaat
     2221 gttgaacgcg tcaatatcaa gcgtgattca aacaaggaaa tggtgctgaa tctcggagtg
     2281 aaattgtaca atcccaatgg gtacaaaatc aaggtgatta aaagtcagct cgatttaacg
     2341 atttcaggta caagcgcagg aaaagcgatt ctcgaagaca aagtgattct gcgcaagcga
     2401 aaggaagaag tgtacgatat caatatttcc atcgatcgtt ctgcagtaac caaagcattg
     2461 atggcttccg gaattaattt gctttttaca gggaaagttc cggttgaagt aaagggttgg
     2521 atcaagggta gggtgtttat tttcggaaag aaatttgatg ttgccttcaa agagcagatc
     2581 gatccgaagc agttcaagat gaattgatat gcccaatcgt ctttcgactg agagtagtcc
     2641 gtatttacgg caacatgcag agaatcctgt cgactggttt ccctggggcg aggaggcatt
     2701 ttccaaagcc aaagcagaag gaaaacttgt attgatcagc attggttatt catcctgcca
     2761 ttggtgtcat gttatggagc atgaaacatt ttcggatgtt gctgctgcgg aggtgatgaa
     2821 ccgttatttt atctgtatta aagtagatcg cgaagagcgt ccggatatcg atcaggtgta
     2881 tatgaatgcc gttcagctga tgagcggaca agggggatgg ccattgaatt gttttgtgat
     2941 gccggatggt cgtccggttt acggcggaac gtatttcccg agggaaaact ggctgaaagt
     3001 gctgcagcat ctcaatgcga tttggaagga cgaacccgaa aaagtggaag attatgcaca
     3061 gcgcttaacc gaaggagttc agttaagtga tctcgccatt cctgaagaag ggtcgaatac
     3121 attttctgca gcatatgcgg atcaggtggt tcagcgttgg aagaatcatt tcgattcgat
     3181 cgaaggtggc cccaatcgtg cgcctaaatt tccattaccg aacaattacg aatttttgtt
     3241 gcgttatgcc atttacagaa acgatgattc ggtaatgaag catgttcgtt taacactgga
     3301 taaaattgcg ttaggcggaa tttacgatca gctgggtggt ggtattaccc ggtactccac
     3361 cgatatgatc tggaaggttc cgcattttga aaaaatgttg tacgataatg cgcaaattat
     3421 ctccttatac agcgaagcat ttcggtttac gcgcgatccg ctttatctgg aaattgcaga
     3481 gcgaagtatc cgtttttgta tagaggaatg gtcgaaaccg gaaagtggtt tttattccgc
     3541 attggatgcc gatagcgaag gtgaagaagg gaaatattac gtctggaaaa aggacgaatt
     3601 gcagggattg ctgggagaag agtacagttg gttttcggag tattacaaca tcaattatct
     3661 gggtgagtgg gagcacggaa attatattct acttcgcaga gaacgtgatg atgcatttgc
     3721 acgaaaacac aaaatggata tcgatgattt tcttcagaaa aaaaagaaag cgcatcacct
     3781 gttaatgacc gtacgaaaga aacggatctc tccattgctg gatgataaaa ttctttgttc
     3841 ctggaatgcc ttaatgatca ctgcgctttg cgatgcatgg aaatgcagtg gaaagaatga
     3901 atatcgggat aaagccatct caacagcaca ttttattctg cgtgaaatgc tgaagggaga
     3961 tggaggcttg tatcattgtt tccattcggg aaaagcgtat gtggatggat ttctggagga
     4021 ttatgcgttt tgcatcgagg cgtttacccg attgttttcc attacaggtg atgaactttg
     4081 gctggcctat gccgataagt gggcaacgta tgtgctccat tatttttctg atgaaaatga
     4141 catcttgttt tattttacct cttcaaaatc ggaagcactc atagcacgca aaacggaatt
     4201 gtcggataat gttattcccg cttcaaattc tgttatggcg cgttccttgc atgagttggg
     4261 cggtattatg ggtaagattg aatatacaca cagagccaat agaatgctgc aaaccgttta
     4321 ttcgcatatg gaagcttatg gtgccggata ttccaattgg ggaatgctcc tgctcgatct
     4381 tttggaggaa ccgaaagaag tggtggtttg tggtgaggat gccgacacga tggccttgag
     4441 gattttacag gatgttccgc cgcatgtgcg ggtgtatgtc tgcatagcgg aaagcgactt
     4501 gcctttgttc aaaaacaggt tcaaaaccgg acaatccctc atttacgttt gccgcaacaa
     4561 atcctgtaat ttgccggtgc aaagtccgga agaagcattg gaacaattga catgaaagca
     4621 agcggaatta tctttttttt ctttttactg tcggtgcatt tgcctgcgca gattatcgat
     4681 aacgcgggcg gaacggcatt taccgacgat aactttttta attcttcctt tattcgtacc
     4741 aacaaggtgc agattatccg cggtagcgta agcaccaaac gcgagggaga acccatgatt
     4801 caacgcagtc agtttttcca ttacgaattc gataccaagg gaagaattat tttaatgatg
     4861 tcgacctttg cttcgtacag cagcaatcgc gatactacca ttattcagtt tgagtacaac
     4921 gacaaaggac tgctggcgac caaacgtaga aatgataaca acggatttta ttcctataac
     4981 tacgaatacg attcgctggg cagattgatc aaagaaacct attgcaggga tgagaattgc
     5041 ggggtggacc gctaccattt taagctggga caacgttttg tgatttcttc tgaaacttat
     5101 agctatcacc aatatgtaaa gaatcagaag gtgaagaaat tttataataa ctacggactc
     5161 caataccagg aagaattttc gtattacaac gaattgggtt atctcaccga ggaagttttg
     5221 cgtcttactt taagcgggaa agaatcgaaa attcagtacg aatacgatga gaagggaagg
     5281 ataagtaaaa agaacgatat cagttatgtt ttcggattca acgaaatcag caatacatac
     5341 acctacgata aattagggaa tttggatgag caggctattt tccataatgg aaagcagaaa
     5401 acattacggt cgatgttgta cgataaaaca acgtatctga tgaccgccca ggtgatgaaa
     5461 gatttcgaga cgggattgat ttatgtgttt aaatattcgt acgagttttt ttaggagaag
     5521 atcctaaaat aactaaacga aaaagccccg cataaatgca gggcgattcc gaacaaacaa
     5581 caaaccaaac taagccacac tttgctacct cctgtgtgta ttggcatctt taaggcttaa
     5641 gcctttttcc ttgacttcag gtgaaggagc gttcccggtt cgggttctcc tgtgcctgga
     5701 gtaatattgt tgagcttata aagtttgctc agttttatcc cgtatttctg ggagatgtta
     5761 tgcattgtgt ccccctcctg aacacgatgc gtttcaacct gagctttatt tcttttgggt
     5821 tggagataaa taatatcccc gggttgcaga acgtcttttt tacttagatc gttgtatttg
     5881 tagatctgcc acaaacccaa ttcaaattct tttgaaattt tataatacgt gtcgccatct
     5941 tttgcaatga tgaacttgat gtggttatcg tgtactttaa ccttgtgctg aatctgattg
     6001 atcccgttga attcttcctg acgcgcaaca cttggttttt ttgcggcaat gttgtttacc
     6061 acatcataat gattcaatcc attgtcttcg atgatctgaa ttagaatatc tgcgtattgc
     6121 ggattggttg cataaccagc ttttttcaat ccttttgccc aaccgcgata atcggtctga
     6181 tcaagtgtga ataaagaagc ataacgactt ctgttcttca gaaacagaga atgatcgcga
     6241 tacgattcac cggctgtatt gtatttgcgg aaacactcat ccctgcggtc gtcgtcctgt
     6301 ataaacgttt ctccgttcca gttatcatga catttaattc cgaaatgatt gttggcataa
     6361 cgtgcgagtg gactatttcc atctccgctt tcgagaatac cctgcgcaag tgtaatggat
     6421 gcgggcacgc cgtattcgag catttgacgc tgagcttcat ccacccattg cgatacatac
     6481 tcttttctgg aaatccttct ttccgcgggc tgtgcattgg cctggatttg gaatattccg
     6541 gccacgaaca gtgctaacga tttgaatacc agtgatttca ttgcttgttt gtttattgtt
     6601 tgttgattgc ttgttgaaaa caggtagtct aacgcaggtt tttaacatag gtttcagaat
     6661 tgttaatatc tttttgaagt aaattatagg taagctttat aaatagctga attttagtgt
     6721 tttaagattt tttgtatatt ttaaattttt cctttttatc ttgcataaaa aaactgcgat
     6781 gagaattatt gttctgataa tcatgatttt aggcgtttca gggtcttgcc tggctcaaat
     6841 aaagcaagta gcgcgactgg aacttgaatt gtatgaagaa ggagccgatc ataccgatat
     6901 actgggacct catggtctta ttgttcacgg acgtatcaat attaaaggga gaggtgacct
     6961 gtggtcgatc cgccgattca atacggattt aaaacttatc gattctaccg gttttgcgat
     7021 tagtgaacga atgattctta cccaaagttg gttaacagat gatttttcgg ccttgtattt
     7081 tttgtttacc gaaaaggcaa agcgaactta ttataaacta ctccgcttcg atactccaac
     7141 cggaaaaatg tctgttaaag aagctgaatt acccaaagga acctttttta ataagatgag
     7201 tgttgtaggg aattcaattt ttttgtcggg cactttagat aaaaaaacag ctttattcct
     7261 tttcgacttc gaagcaggtt cctttacaaa tgtcaattat attcctcttc aaaaatacag
     7321 cgttataggg atggatgtgc ctgccgaaga acccatgtta atggtaaccg atgcaaaggg
     7381 aatgggtaag ggcttttctt tccgggttag tttttttgat gagaagggaa ttcccacatt
     7441 taaaccacaa gctgttattt cacaacccga ttattattgc cgaagcgcat ctttaacgtt
     7501 gttgggtggt aatgagtata ttttgacagg ctcctattcc caaggaaaaa aaagtactgc
     7561 aaacggggtg tattttacac gctatgaaaa cgaccagcaa ataatgttga aataccacag
     7621 tttttcaggc tttaaaaatt tctatgaata cctgaatccg cgtcaaaaag aaagaatgga
     7681 aaaaaaggcg gaaaagaaag cgaagaaagg gaaagaagag ttcattgaag cctatatcgc
     7741 aagtcatccg gtttttacat ttaatggaaa ctatatgtat gtgggtgaaa tttattaccc
     7801 cacataccgt acagaggttt attatacaac tggaccaaac ggccaaatga tgcgtcagac
     7861 tcgtcgtgtt tttgatggct accagtatac ccatgctgtt gtattggcaa tggataagga
     7921 tggaaacaaa gtgtacgatc attgttttcc gatgtggtca ggatttaaac caatgacagt
     7981 tcgtcgattt ttaagaattg ttgttggaag cgatcaagct gatttgttat ttgggaatgg
     8041 ctcaactgtt aaagggatgc ggatcaccgg taataatatt gccgaactca attatggaga
     8101 aattgaaacc ggaaatgaaa acgataaagt tcgttggaca tccatgactc agacttcgta
     8161 ttggtacgat aactatttca tctcttacgg tagccagtcc attaagaatt ccgatgaaaa
     8221 tggaaagaaa aggcgtactg tcttttttgt gacgaagatg cagttttgat ccctcgattg
     8281 aacttttttt atgtatgtta aattgttaac ccgataacga aaagattgga gagcaagaat
     8341 catcctttgc tttggaagta ttgaaaaaga tatataggga gcaacattga gcaaattcga
     8401 gctttgttta attcggtgga aaccattgaa taattaaggg gaccatttat ggtcaccaat
     8461 ggctaattgt ttttatttaa tgtatttttt tctatattag ttgcaatcaa acttattatt
     8521 atgattaagc aactgttttt tctaacctgt ttattcctga ttgtttttcc tggccggtct
     8581 cagatgagag aggtggctcg ggtggagaat aaatataggc aaggcgcaga ttatatgttt
     8641 ttaaaccgtg aaaatggaat tgttcttgga ggtcttgttt ttgctgaaga taagaaaccc
     8701 atgacgtatg catttaaaca ttacaatact gatttaaagc taacagattc agttgtattt
     8761 gaagtcccaa tgggtttcgc tttatttgga aataggaaaa ttgaagatga tatttatttt
     8821 gtttttagta cctataataa gacaccggta tatattattg ccagatatcg aataccggaa
     8881 aaaaaggttg aactaaagcg gggcacttta cca
//
LOCUS       DAOMYG010000067         8899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2609_length_8899_cov_9.996495, whole genome shotgun sequence.
ACCESSION   DAOMYG010000067 DAOMYG010000000
VERSION     DAOMYG010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8899
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2609_length_8899_cov_9.996495"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PK610_03445"
     CDS             complement(<1..312)
                     /locus_tag="PK610_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine lyase"
                     /protein_id="HRJ35010.1"
                     /translation="MSLEKHFSTFRENIIGQHQWFTGPEGEKRILYADWTASGRMYAP
                     IEDLLKNEIAPFVGNTHTETSVCGTTMTKAYHEALHIIKHHVNAGKDDAIITYGSGMT
                     AV"
     gene            complement(319..735)
                     /locus_tag="PK610_03450"
     CDS             complement(319..735)
                     /locus_tag="PK610_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRJ35011.1"
                     /translation="MKKNLFFLVIGVVFGTILTKGEVISWFRIQEMFHFQSFHMYGVI
                     GCAVVVGALSVQLIQRFNIKTIHGERINLEKKPAQYKAHILGGTLFGMGWALTGACPA
                     PIYALTGAGYYPMITVLFSAVMGTFTYALVKDKLPH"
     gene            complement(738..1295)
                     /locus_tag="PK610_03455"
     CDS             complement(738..1295)
                     /locus_tag="PK610_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRJ35012.1"
                     /translation="MLEILKQPWPWYITGPLIGLMVPLLLIFANRHFGISTSFRHICA
                     ACIPTKNPFFKYDWKKESWMLFLAGGIIIGGWIGGDALKNPNPMQLSNNAIELFASMG
                     FSDFTEISPSAIFGWESFSGWQGWLFMIAGGFLIGFGTRWANGCTAGHAIMGLSLLNP
                     GSLVAVIGFFIGGLAVTHLLFPQLF"
     gene            1508..2752
                     /locus_tag="PK610_03460"
     CDS             1508..2752
                     /locus_tag="PK610_03460"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HRJ35013.1"
                     /translation="MKQFFSCLLLMTLAASAYAQEKAKAYWTIESALQDPKKVKILIL
                     SDKDLTEVPGSIEKLINLEELSLNNNKLTVLPQWISGLKQLRKLDLSNNKLQSVPEVI
                     RDIASLKELRLEDNLLFTLPSWLGSMATLEKLDVSGNKLKMLTPDAGFPKSLLSLNIS
                     SNEMALFPVAVISCSSLRELMISRNKIKVLPELGALKNLEYIDFSFNQIDRIPASMSG
                     CSKLEIVKLRGNKLTEIQFLQAKTITSLDLGANAFVSFPSEIISMDSLQELLIDQNKL
                     NAIPDAFSKLQMIGRLDISTNAFPFFPEIICSLTRLKELRASANKFSSLPSCFSSLTQ
                     LTDLDLGKNAFTAIPSMLGKMQALSTIDFSDNKIKQVFDELSAWKHKVEIKMWGNGIL
                     REELAKFPDGELSQYVTLVLLK"
     gene            complement(2818..4215)
                     /locus_tag="PK610_03465"
     CDS             complement(2818..4215)
                     /locus_tag="PK610_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ35014.1"
                     /translation="MKIEQLYTNCLAEAAYYIESEGEAVIIDPLRETDPYTKKAAENN
                     SKIKYILETHFHADFVSGHVDLSKKTGATIVYGPTAQPAFTCHIAKDGEVLKVGKITI
                     TVLHTPGHTPESVSYLLKDENGKEHALFTGDTLFIGDVGRPDLAVKSNLTEKDLAGML
                     YDSLREKIMTLPDAVIVYPAHGAGSACGKNIGKETYSTLGEQKKNNYALQPMSKEEFI
                     KEVTTGILPAPQYFAKNAALNKGGYDSIDDVIERGNKALTIDAFATEIANGALVLDVR
                     KKEDFAAGYIPGALFIGLDGQFAVWVGALIKDLNQAILLICDEGREEEAVLRLARVGY
                     DNCKGFLKGGFSTWKNSGKAIAVIEQIKPENLAKAYATASIIDVRKPGEFEAEHVENA
                     ISLPLDFIAEKMNVLDKNKHYFVHCKSGYRSMIAVSILKANGITNLVDVIGGYDAIAK
                     TEVPVSAFVCPSTLK"
     gene            complement(4257..4688)
                     /locus_tag="PK610_03470"
     CDS             complement(4257..4688)
                     /locus_tag="PK610_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010523221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HRJ35015.1"
                     /translation="MLFAKGTKMYSIAHKKCPHCHEGEFFVSRNPYNLSKAGEVNENC
                     SVCKRKLSIEPGFYYGAMYVAYALAVAMYVSIYVATNVLYPSASTLVQILLIIGGLIL
                     TGPWLYALSKMIWGNFFIAYKGVEPTEKEILELGKRQVPKA"
     gene            complement(4703..5506)
                     /locus_tag="PK610_03475"
     CDS             complement(4703..5506)
                     /locus_tag="PK610_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRJ35016.1"
                     /translation="MLVIAGYIGAVLMGIVLGLIGGGGSILTFPVLVYFFEIEANHAT
                     SDSLFIVGITSFIGAIGYLKNQLVSMRSVLVFGLPSIVSVYFTKRYIFPPIPGEFPVF
                     GVIVSKDFAVTVLFALLMVIASFSMIKPIKVIRQDDYESTERYRYLLIMIIGLAVGFL
                     AGMVGAGGGFLIIPALVILAKLPMKKAIGTSLVIIFLNSLLGFAGGLSADANVNWNLL
                     LSLAALSIGGILIGLKLAKNIPGSKLKPAFGWFVLATGSLMLIDKFFNH"
     gene            complement(5506..5793)
                     /locus_tag="PK610_03480"
     CDS             complement(5506..5793)
                     /locus_tag="PK610_03480"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRJ35017.1"
                     /translation="MKKQELAKLENASNILKTVGHPMRIAIIELLRANRGLTVTEIHE
                     SLGILQAIASQHLALLKEIEVVGFRRKGRNVRYFISNKKVYKLIELAKGFN"
     gene            5904..7118
                     /locus_tag="PK610_03485"
     CDS             5904..7118
                     /locus_tag="PK610_03485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HRJ35018.1"
                     /translation="MNHERPPHFPLIDAVCTALAEIFEQRYYADKVIERTMKLNPKMG
                     SRDRAFLAETTYDVVRWWRLLHYCAATNEQTDRPSLLRVLSAYFFLFHDKKYAWKENS
                     GMDFSSVRQKHTEALAVRALRYSIPDWMDEMGERELGLQWEKELVALNEEAPVDVRSN
                     RLKTNPDALSMFLQEDGIETERVEHAPDALRLTERRNIFRNKWFLEGYFEVQDAGSQQ
                     IAAYLEVKPGMRVIDACAGAGGKALHLAALMENKGRLIAMDVEERKLQELKKRSARAG
                     VSIIEPKIIDSTKVIKRQEASADRLLLDVPCSGLGTLRRNPDAKWKLSPEFLEKVKKL
                     QKEILFSYAKMLKPGGKMVYATCSIFPSENHEQVKAFLAENPSFKLVHEQKLSPALTG
                     TDGFYMALIRKI"
     gene            7299..8000
                     /locus_tag="PK610_03490"
     CDS             7299..8000
                     /locus_tag="PK610_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HRJ35019.1"
                     /translation="MSNSALLKSSLVKKYWMSATGLFLCLFLAGHLAGNLQLLISPEN
                     GARDAFNMYGKFMTTNIFVKVLSIVTYISILFHAIDGIILTIQNRRARPVRYVKETPS
                     ASSSWASRNMALLGTVMLVFIVLHMLAFWKRMHFGPIETYIAANGEELKDLYSITVLS
                     FKDADNGLIVCMIYLVAMIAIAFHLMHGFQSAFQSVGIRHPKYTPLIKKVGYGFALLV
                     PLLFAIIPFYIRFML"
     gene            8018..>8899
                     /locus_tag="PK610_03495"
     CDS             8018..>8899
                     /locus_tag="PK610_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002677508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRJ35020.1"
                     /translation="MSKLNSKIPEGSVDKKWTDYKNHVKLVNPANKRSIDIIVVGTGL
                     AGSSAAASLAEMGYSVKAFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSTYRLFYDTI
                     KGGDYRAREANVHRLAEVSANIIDQCVAQGVPFARDYGGLLDNRSFGGTQVQRTFYAA
                     GQTGQQLLLGAYSALSRQIALGKVKMYNRHEMLDVVVVGGKARGIIARNLVTGEIERH
                     SAHAVLLCSGGYGNVFFLSTNAMGSNVTAAWKAHKRGAFFGNPCFTQIHPTCIPVSGD
                     HQSKLTLMSESLRNDGRI"
BASE COUNT         2507 a         1868 c         1923 g         2601 t
ORIGIN      
        1 caccgctgtc attcccgaac cataagtgat tatcgcatcg tctttaccgg cgttgacatg
       61 atgcttgatg atgtgtaagg cttcatgata ggccttggtc attgttgttc cgcaaacgga
      121 ggtttccgtg tgggtattgc ctacaaatgg cgcgatctcg tttttcagca aatcttctat
      181 gggtgcatac attcttccgc tggctgtcca gtcggcataa agaatgcgtt tttcgccttc
      241 cgggccggtg aaccattgat gttgtccgat gatgttttcg cgaaaggtgg agaaatgttt
      301 ttcgagcgac ataaaaattt aatgaggcag tttgtctttg acaagtgcgt aggtaaatgt
      361 ccccatcact gcgctaaaca gtacagtgat catggggtag tatccggctc cggtcaatgc
      421 atagatgggg gcaggacaag ctcccgtaag tgcccatccc attccgaaaa gtgtacctcc
      481 cagaatatgc gctttgtatt gtgcaggttt cttttcgaga ttaattcttt cgccatgaat
      541 ggttttgatg ttgaaacgct gaatgagctg aaccgagagg gctccaacca caacagcgca
      601 tccaattaca ccgtacatgt ggaaactttg gaagtggaac atttcctgaa tgcgaaacca
      661 cgaaatgact tcccctttgg taaggatggt tccgaatacc actccaatga cgagaaaaaa
      721 cagattcttt ttcatgttca gaagagttga gggaacagaa gatgagtaac agcgagtccg
      781 ccaatgaaaa acccgataac agcaaccagc gagccgggat tgagcaatga taatcccatg
      841 atggcatgtc ctgcagtaca tccattggcc cagcgagttc cgaatccgat caggaatcct
      901 ccggcaatca taaacaacca accctgccaa ccggaaaacg attcccaacc aaagatggct
      961 gaaggcgaga tctctgtgaa atcagaaaac cccatcgaag caaacagttc aatagcgttg
     1021 ttactgagtt gcatcggatt ggggttctta agcgcatctc cgccaatcca gccgccaatt
     1081 attattccgc ctgctaaaaa caacatccac gattcttttt tccagtcgta cttgaaaaat
     1141 ggattcttcg taggaataca ggcagcacag atatgccgaa aagaagtgga tattccaaaa
     1201 tgacggtttg caaaaataag caagagcgga accatgagcc cgatgagcgg acctgtaatg
     1261 taccatggcc agggttgttt taatatttcg agcatcgaat gatcttttgt caaaaataga
     1321 atgaaatatg gtcatgcaat gatgttgttt aaagtatttg ccctatcgtt tttgttaata
     1381 gatcttgttg ttggtttgga tcacaaacat ttacagttta ttatgcggct tacatcttaa
     1441 gatctcaaca ataccctaat aattgttccg atatacctac atttaattga ataaacggaa
     1501 gggttctatg aaacaatttt tttcatgttt gttgttgatg acgcttgctg cgtctgctta
     1561 tgctcaggaa aaagcaaaag cgtattggac tatcgagtct gcattgcagg atcccaaaaa
     1621 ggtaaaaatt ttaattcttt ccgataaaga cttaacagag gttcccggtt caatcgagaa
     1681 attaatcaac ctcgaagagt taagccttaa caacaacaaa ttgactgttt taccacaatg
     1741 gatttccgga ttaaagcaat tgcggaagct ggatctttcc aataataagc ttcaatctgt
     1801 tccggaggta atcagagata ttgcctcttt gaaggagctg cgcttggagg ataatttgtt
     1861 gttcactttg ccttcctggt tgggatcgat ggcaacattg gaaaaattgg atgtttccgg
     1921 caacaaatta aagatgctga cacctgatgc cgggtttccg aaatcgttac tgtctctgaa
     1981 tatttcttca aatgaaatgg cgctttttcc ggtggccgta atttcctgtt cctccttgag
     2041 ggaattaatg atcagcagaa ataaaataaa agtacttcct gagttgggag cattaaaaaa
     2101 cctggagtat atcgattttt cgttcaatca aatcgatcgg attcctgcgt caatgtcggg
     2161 ctgttccaaa ttggaaattg taaaactcag agggaataaa ttaaccgaaa tacaattctt
     2221 acaggcaaaa acgattacaa gtctggatct tggcgccaat gcatttgttt cgtttccttc
     2281 agagattatt tcaatggata gtttacagga attattgatc gatcagaata aattgaacgc
     2341 cattccggat gcattttcga aattacaaat gatcggcaga ctcgatatta gcaccaatgc
     2401 gtttcctttt ttccctgaga ttatctgttc actaaccaga ttaaaggaac taagggcatc
     2461 agcaaataaa ttttcgtcct taccttcttg tttctcttca ttgacccaac ttaccgatct
     2521 tgatctgggt aaaaatgcat ttactgctat accatcaatg ttaggcaaaa tgcaggcctt
     2581 gtcgacaatc gatttttctg acaataaaat caaacaggtt tttgacgaac tatcagcttg
     2641 gaaacacaaa gtcgagataa aaatgtgggg aaatggaatt ttgcgggaag agcttgcaaa
     2701 atttccggat ggtgaattgt cgcaatatgt taccttggtt ttattgaaat gaaaacgaaa
     2761 aatttggcat aaaaaaaggg cgtcatcacg acgccctttt tttatgtgaa agtgatctta
     2821 ttttaatgta gaaggacaaa caaatgcaga tacaggaacc tctgtttttg caatggcatc
     2881 ataaccacca attacgtcca ccaggttcgt tattccattc gcttttagaa tggatacagc
     2941 aatcattgac cggtatccgc ttttgcaatg aacaaaatag tgtttgtttt tgtcaagtac
     3001 gttcattttt tctgcaatga aatccagagg aaggctaatt gcattttcca cgtgctctgc
     3061 ttcgaattcg ccgggtttgc ggacatcaat gatgctggcc gttgcatacg cttttgccag
     3121 attttccggc ttgatctgtt caataacagc aattgctttc ccggaatttt tccaggtcga
     3181 aaaaccacct tttaaaaacc ctttacaatt atcataacca actcttgcca aacgaagaac
     3241 cgcctcttct tcacgcccct catcgcaaat aagaagaatt gcttgattaa gatccttgat
     3301 caaagcccca acccaaacag cgaattgacc gtcgagacca ataaatagtg cgccgggtat
     3361 atatcctgct gcaaaatctt cttttttacg cacatccaaa accagtgctc catttgcaat
     3421 ttccgtggca aaagcatcaa tggtgagagc tttgtttccg cgttcaatta catcatcgat
     3481 actgtcgtaa ccccctttgt tgagcgctgc attctttgca aagtattgtg gagcaggtag
     3541 aattcctgtt gtgacttctt ttataaattc ttccttgctc attggttgca aggcatagtt
     3601 gtttttcttt tgttctccta atgttgaata cgtttcctta ccaatattct taccgcatgc
     3661 tgaaccagct ccgtgcgcag gataaacgat tacagcatcc ggaagcgtca tgattttttc
     3721 acgtagggaa tcatataaca ttcctgcgag atctttttca gtcagattcg attttaccgc
     3781 aaggtccgga cgacccacat ctccaatgaa aagcgtatca ccggtgaaaa gtgcgtgttc
     3841 ctttccgttt tcatctttca gcaaataact taccgattca ggagtgtgtc ccggtgtatg
     3901 caacaccgtt atggttattt tgcccacttt aaggacttca ccgtcttttg caatatgaca
     3961 ggtaaaggca ggttgggctg ttggtccata gacaatggtt gcccctgttt tttttgaaag
     4021 atcaacgtga ccggaaacaa aatcggcatg gaagtgggtt tccaggatgt attttatctt
     4081 gctattgttt tcggcagctt tctttgtata cggatcggtt tcacgtaagg gatcaatgat
     4141 cactgcttca ccttcgcttt cgatgtagta ggctgcttct gcaagacagt tggtatagag
     4201 ttgttcaatc ttcatatgga taggattctt ccggtcaaca aatttagtga attcgattat
     4261 gctttaggga cttgtctttt acccagttcc aggatttctt tttccgttgg ttcaacgccc
     4321 ttataagcaa tgaaaaaatt accccatatc attttcgaga gcgcataaag ccatggtccg
     4381 gttaggatca atcctccaat gatcaataga atttgcacca atgtacttgc agatggatac
     4441 agaacatttg ttgccacata tatacttaca tacatcgcta ctgcaagtgc ataagccaca
     4501 tacattgctc cgtagtagaa gccgggttct atgctgagtt tccgtttaca aacactgcaa
     4561 ttctcgttaa cttcccctgc cttggaaagg ttatagggat tacgggaaac aaaaaattct
     4621 ccttcatggc agtgcggaca ttttttatgt gcaatgctgt acatttttgt gcctttagcg
     4681 aatagcataa gagggaagat gattaatgat taaaaaattt gtcgatcaac atcaggctgc
     4741 cggttgcaag aacaaaccat ccaaatgccg gcttgagttt tgatccggga atgttctttg
     4801 cgagcttgag tccaatcagt attcctccaa tcgataaggc ggcaagactg agtaacaggt
     4861 tccagtttac gtttgcatcc gcgctgaggc caccggcaaa tcccagcaat gagtttaaga
     4921 agatgatcac aagagaagtt ccaattgctt ttttcatggg tagcttggca aggatcacca
     4981 aagccggaat gatcagaaat ccgcctcctg cacctaccat gcctgcaaga aaacctaccg
     5041 ctaaaccaat gatcatgatc agtaagtaac gataacgttc tgttgattca tagtcatctt
     5101 gccgaattac cttgatcggt ttgatcatgg agaaggaggc gataaccatg agtaaggcaa
     5161 agagtactgt tactgcaaaa tccttactta cgataacacc gaaaacagga aattcgccgg
     5221 gaataggtgg aaaaatatag cgcttggtaa agtaaaccga aacaatactc ggtaaaccga
     5281 aaaccaacac cgagcgcatg ctaaccagct gatttttcaa atagcctatc gcccctataa
     5341 atgaggttat tccaacaata aataaggaat cggaagtagc gtggttggcc tctatttcaa
     5401 agaaataaac caataccggg aaggtgagta tggagccccc tccgccaata aggccgagaa
     5461 caatccccat caacaccgcc ccgatataac cggctataac aagcattagt tgaagccttt
     5521 agccaactca attagtttgt aaaccttttt gttactgatg aaatacctga catttctgcc
     5581 ttttctccgg aacccaacaa cttcgatctc tttgagcaat gccaaatgct gcgaggcaat
     5641 tgcctgcaga ataccgagtg attcatgaat ctccgttact gtgaggcctc tgttggcacg
     5701 gagcagttca ataatggcaa tcctcatggg gtggccaact gtttttaaga tgtttgatgc
     5761 gttttccagc ttagccagtt cttgcttttt catagttctg tagtgttttt tgcaaacata
     5821 ggtagaatcc tatgtttttt gggtgacttt tatcacatct ttagtttttt ttcagccttc
     5881 cttaaacttc ttacttttgg actgtgaatc acgaacgccc tcctcatttt ccgcttattg
     5941 atgcagtttg tacagcactg gcggagatct ttgaacaacg ttattatgcc gacaaggtca
     6001 ttgaaaggac catgaagctc aatccgaaga tggggtcgcg cgatagagcc tttcttgctg
     6061 aaacaaccta tgatgtagta aggtggtggc gcttgttgca ttattgtgca gcaacaaatg
     6121 aacaaacaga tcgaccatcc ttgcttaggg tattatcggc ctattttttt ctctttcacg
     6181 ataagaaata tgcctggaaa gaaaattccg gaatggattt ttcatctgtc cgccaaaaac
     6241 atacagaagc cttggcggtt agagcgttac gctacagtat tccggattgg atggatgaaa
     6301 tgggagaacg ggaattggga ctgcaatggg agaaggagct tgttgcttta aacgaggagg
     6361 caccggtaga tgtcaggtca aaccgattga aaaccaatcc cgatgcgctt tctatgttct
     6421 tgcaagaaga tggtatagaa acagaaaggg ttgaacatgc gccggatgca ttgcggctca
     6481 cggaacgaag aaatattttc agaaacaagt ggtttttgga agggtatttt gaagtgcagg
     6541 atgcaggctc ccaacagatt gccgcatatc ttgaagtgaa accgggaatg agggtcattg
     6601 atgcttgtgc aggtgcagga ggaaaagcac ttcatcttgc tgccttgatg gagaataagg
     6661 ggcggcttat tgccatggat gttgaagaac ggaaacttca ggagttgaaa aagcgttcag
     6721 ccagagccgg agtgagcatt attgagccaa aaattattga ttccactaag gttattaaac
     6781 gtcaggaggc aagcgccgat cgtcttttgt tagacgtgcc ctgttcgggc ttggggacat
     6841 tgcgccgtaa tccggatgca aaatggaagc tcagtcctga atttttggaa aaagtaaaaa
     6901 aacttcaaaa agagattttg ttcagctatg caaaaatgct gaagcccgga gggaaaatgg
     6961 tctacgcaac ctgtagtata tttccatctg aaaaccatga acaggttaaa gcattccttg
     7021 cggaaaatcc ttcctttaaa ttagtgcatg aacagaaact tagtcccgca cttaccggta
     7081 cagatgggtt ttatatggca ttgattcgga aaatttaaca ttgagcatta acttttttgt
     7141 ccgacaattg ttttctattc aatcaattgt aatcggaaaa cgaatcttat tcaaatgagc
     7201 accagacaaa attattagaa tcattctaaa taactttttt ctgtggttca atcgctttgt
     7261 gcggttacat ttgtgcaacc aaaacctcaa ttagcgctat gagtaattct gccctgctca
     7321 agtcctctct ggtaaagaaa tactggatgt cggcaacagg tttgttcctc tgcttgtttc
     7381 tggccggaca ccttgccgga aaccttcagc tcctgatcag tcccgaaaat ggcgcacgtg
     7441 acgcctttaa catgtacggg aaatttatga cgaccaacat ttttgtcaag gtcctcagta
     7501 tcgtcactta catttcaatt ctttttcatg cgattgatgg tatcattttg actattcaaa
     7561 acagaagagc acgtcctgtt cgttatgtta aggaaacacc atctgccagc agctcctggg
     7621 cttctcgcaa tatggcatta ttgggaacag taatgttggt atttatcgtt ttgcatatgc
     7681 tggcattttg gaagcgaatg cattttggtc caattgaaac atacattgct gccaatggcg
     7741 aggaactaaa ggatttatat tccatcaccg ttctttcatt taaggatgca gacaacggac
     7801 ttattgtttg catgatctac ctggtggcca tgattgcaat tgcattccat ttgatgcatg
     7861 gattccaaag cgcatttcag tcggtgggaa ttcgtcaccc aaaatacact ccccttatta
     7921 agaaggtcgg atatggcttt gcactactgg tgccattgct gttcgcgatc attccgttct
     7981 atattcgttt catgttgtaa aaataattta gctgatcatg agcaagctga attccaaaat
     8041 acctgaaggt tccgttgaca agaaatggac cgattataaa aaccacgtaa agctggtgaa
     8101 tcctgctaat aagcgttcta tcgatattat tgtcgttggt acaggtctgg caggatcttc
     8161 tgcggctgct tctcttgctg agatgggtta tagtgttaag gcgttctgtt ttcaggactc
     8221 cccgcgtcgt gcccactcta ttgccgcaca gggagggatc aatgctgcta agaattacca
     8281 gaatgatggt gactctacct acagattgtt ttacgatacc ataaaaggtg gagattaccg
     8341 cgcacgtgaa gccaacgttc atcgcctggc tgaggtttct gcaaacatca tcgaccaatg
     8401 tgttgcacaa ggtgttcctt ttgcacgcga ttacggtggg ttgctcgata accgatcctt
     8461 cggtggaaca caggttcagc gtacgtttta tgcagccggt caaaccggac aacaattatt
     8521 actcggggct tattcagctc tctcaagaca aatagcatta ggtaaagtaa aaatgtacaa
     8581 ccgccatgag atgcttgatg ttgtggttgt aggagggaag gcacgaggaa ttattgcacg
     8641 taaccttgta acaggagaga ttgaacgtca ttcggcccat gcagttttgc tgtgttcagg
     8701 aggttacgga aatgtatttt tcctctctac caatgcaatg ggaagtaacg tcactgctgc
     8761 ctggaaggcg cacaaacgcg gtgctttctt tggaaatcct tgttttacac aaattcatcc
     8821 gacctgtatt cctgtttccg gcgaccatca gtcgaaactg actttgatgt cggaatcact
     8881 tcgaaacgac ggacgcatc
//
LOCUS       DAOMYG010000068         8898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2610_length_8898_cov_9.201741, whole genome shotgun sequence.
ACCESSION   DAOMYG010000068 DAOMYG010000000
VERSION     DAOMYG010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8898
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2610_length_8898_cov_9.201741"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1112)
                     /gene="gldN"
                     /locus_tag="PK610_03500"
     CDS             complement(144..1112)
                     /gene="gldN"
                     /locus_tag="PK610_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HRJ35021.1"
                     /translation="MKRYLLIVMALVALGTPVSAQNVLDGVYVKEHIPTKKLIQYPHL
                     READVMWEKRIWRVIDTREKINFPLYYPLKRLTDRENLFDILRKHVLAAEIIPYNYVG
                     PDLTEYGGDMFFYPISPSLPDYREQANALFGEPEAPPTTPMVDQNGNQIVLGSDEYGY
                     PIYQYHPADKVIGKTPRGGDSIMPGRDTIYYSAEDIIGWEVKEDWFFEKQRSVMDVRI
                     IGIAPIVGSKNVNSLGVPRKLFWIYFPELRYIIQNYFVYNRQNDAQRMSFDDLFWKRM
                     FNSYIKKESNIYDRQIIDYTSGIDALLESEKIRIQMQDIEHDVWDI"
     gene            complement(1143..2870)
                     /locus_tag="PK610_03505"
     CDS             complement(1143..2870)
                     /locus_tag="PK610_03505"
                     /inference="COORDINATES: protein motif:HMM:NF023504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HRJ35022.1"
                     /translation="MAGGKETPRQKMIGMMYLVLTALLALNVSKEIVVAFITLNDKLD
                     QSTAAIEYKNAVGYQDFENYYNVPQTRELVKVWRPKAQQIEKLANRVHDFYLDETNKL
                     IKAVEGDAKEWVIQEEGGKRKLANLMDIGSKDDYDAATRLFVGDRSNIVEAGKALMTE
                     MHNFRDSLCYIISTYESGKNNFTFTPKFDGASMSPEELNAALGTANPKDTAKIKQLYG
                     MLTLPATMKNHDEEVPWQAGMFDHAPVVAAAALFTSLRNDVRNAESVAVDHLLGKVNA
                     PKFNFNKIEPLAFARTSYLNVGDSMNLSVMIAAYDSNEVAKIRFAVNDSNPANYKDHV
                     GKLSVQGSRPGAYTMYGLVSVKENGEEKWKPWKYMYEVGEPTGVIANEDLTVIYAGYE
                     HTFSASVSGYPQDRVSLSLPGISVSPKGGGKFSVKAPASLINKKIKAGVTVKTDGGGS
                     KSFAGPEFTVKPLPIPSSYLGNLSSVQSDVSLGELKTNAQFLRVAYDPSISLDPAKVK
                     FTVNSFEMTATVGAASKSFPAKGGQLTPDMLNFIKLAKPGTVIAFNSISAQGPAGGVR
                     VPPMVFKVK"
     gene            complement(2931..3746)
                     /gene="gldL"
                     /locus_tag="PK610_03510"
     CDS             complement(2931..3746)
                     /gene="gldL"
                     /locus_tag="PK610_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HRJ35023.1"
                     /translation="MKPGSKAWKVFMAKLYGFGAAVVIVGAMFKIMHWPGAGAMLVIG
                     LSTEAVIFIFSAFEPIHEDPKWELVYPELALGHMDDHDGHGAIEHHGKKGSVTEELDK
                     MLEEAKIEPELIQSLGNGLRSLTDATHKIGDVSNASVATNEYIDSLRGASKKVEQLSD
                     SYVKASESIMGLTNTQSEGASFGEQMQKLSTNLSALNNIYEMQLKGSSSHLEATSKLY
                     GGINELMQNLHDSLEDTKKYKENMSDLARNLTALNTIYGNMLAAMNINTGSRG"
     gene            complement(3809..5251)
                     /locus_tag="PK610_03515"
     CDS             complement(3809..5251)
                     /locus_tag="PK610_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRJ35024.1"
                     /translation="MKKLLFYSAIAVTLFACSPGNNGELVGVIPRPSWYQNTPYGMLY
                     IPAGSYNMGASDQDVPWAHTNRSKTVSVQAFYMDQTEISNNEYRQFVYYVRDSIARRK
                     LAEEFPEEFLMPRYDDEGNELDASEWDLNWSARLKYFDPDYMPLLSEMYLPDHERYYR
                     RKEFDVRKLMFEYYWIDLQAAAKKGRYNIKTNPEDPEHRTLSNPEDPITKDPVGRDLD
                     LGYRNKKGQNNAIRGHTDRSRFIIKEIINVYPDTLCWISDFTYSFNDPMTNMYFWHPA
                     YDEYPVVGVTWVQAKAFCVWRTQLLNFWLESMGEFYVQDFRLPTEAEWEYGARGGLDL
                     SPYPWGGPYIRNARGCFLGNFKPMRGRYMEDGGFHTVKVTSYHPNDFGLYCMAGNVSE
                     WCETAYDESNYEIIHDLNPERRYDALDHDPPAQKRKVIRGGSWKDIAYYLQTGSRSFE
                     YQDSAKSYVGYRCVMTYLGRGGPDQAEEAR"
     gene            complement(5351..6292)
                     /locus_tag="PK610_03520"
     CDS             complement(5351..6292)
                     /locus_tag="PK610_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRJ35025.1"
                     /translation="MRKFLLSMGAIVMGSVAYGQQDYQLSQFMYDRLSINPGYTGIDN
                     KICATSFFRSQWSGFDGAPKTFLMNIHGPVPLLRGGVGLSLFSDKIGFFNNFSARLNY
                     SYHLSIGNGDILGIGISAGLMKVSLKPDWIAIDPVNLDNSIPDGSQTQSTYDLAFGLY
                     YKSTNWYAGLSATHLTESDLVNLNVKNARHYYVLGGYDFQLPSNPDIMIRPSFRLESD
                     FTSTQLDFNANVLWKNMVWGGLSYRIKDAIVPMLGFQQPLGNDKMPGMIRIGYSYDVT
                     TSEIRDYSSGSHEIMLSYCFNLKPPVKITKSKTVRFL"
     gene            complement(6433..7605)
                     /locus_tag="PK610_03525"
     CDS             complement(6433..7605)
                     /locus_tag="PK610_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HRJ35026.1"
                     /translation="MKDISIFFEPGFIPEFRSESIIQGTLSAHTVFHRSGDFPDLNGM
                     DIAIIGVMEERNSYANKGCSKAPDEVRKFLYELSPAGHDYKIADLGNIKAGATIEDTW
                     YAVSQSVAFLIKRNILPIIIGGSQDITFANYLAYEKLEQTINMVTIDSRINLGDVEDQ
                     ISSRTFLGKIIMHQPNYLFNYCNVALQSHFADARSLELMDKLYFDNYRLGELRKDITA
                     CEPLIRNADILSFDLSAIRHADAPGNAESGPNGLFGEEACQLCRYAGMSDKLTSAGFY
                     ELNPARDSHGQTTHLVAQMIWYLVDGYYNRKKDFPIGDRSLFTKYRVTSPQFDHEILF
                     FKSNRSDRWWMDVPYPPDKRLKFERHHMVPCSYQDYLQASEGEMPDLWWRTYQKLS"
     gene            complement(7612..>8898)
                     /locus_tag="PK610_03530"
     CDS             complement(7612..>8898)
                     /locus_tag="PK610_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HRJ35027.1"
                     /translation="AMLPPMKVGQALEPSEITGTQRFTHHPPRYTEASLVKKLEELGI
                     GRPSTYAPTISTIQNRGYVVKEDREGVRRDYDLLSLKKGKITEEIKSENTGAEKSKLF
                     PTDIGMVVNDFLVKHFGDVLDFSFTATIEREFDDIAQGMVKWNAMIKRFYGPFHKDVE
                     KTLEHSDRATGERELGVDPTSKRKIVVKIGRFGPMVMIGNAEDEEKPRFASLRKDQSI
                     ATITLAEALDLFKLPRDLGMIDGKKVVANVGRFGPYVAHDGVFASIKKTDGDDPMTIT
                     LDRAKVLLADKRQANADRIIKAFPENSEIQVLKGRWGAFLKIGKKNFRIPKDLEPEKL
                     TLEKCMELAGDAGVKELKKGKSDGVTLKKAASKRPAAKKKTSAPRKKTGTASVKKAPA
                     KKAPAKTSVAKKTTGSKTTAAAKKKTTAVRKKTTRR"
BASE COUNT         2451 a         2040 c         1810 g         2597 t
ORIGIN      
        1 ttagcgttta aggacgcagc ataaccataa ccgttttttg aggtattcgg atttagtctt
       61 atactgaatc tatcattgaa aaaagacaat aaacgaaaag ccccggcaaa attgccgggg
      121 cttttgaaaa atattggttg agattaaata tcccaaacgt cgtgctcgat atcctgcatt
      181 tgaatacgaa tcttctcgct ttcgagtaac gcatcaatgc ctgaagtgta atcgataatc
      241 tgacgatcat agatattgga ctctttcttt atgtaactgt tgaacatacg cttccagaat
      301 aagtcatcga aagacatacg ctgcgcatcg ttctggcggt tgtacacaaa atagttttga
      361 atgatgtagc gaagttcagg gaaataaatc cagaacaact tacggggaac gccaagtgag
      421 ttaacgtttt tggatccgac aatcggcgcg attccgataa tgcgaacatc cattaccgaa
      481 cgttgctttt caaagaacca gtcttcctta acctcccagc caataatatc ctcagcactg
      541 tagtaaatgg tatcacgacc aggcataata gagtcgcctc cgcgtggtgt tttaccgatt
      601 accttatctg cagggtgata ttggtagata ggatatccgt attcatcaga ccccagaacg
      661 atttggttac cgttttggtc aaccattgga gtggttggtg gtgcttccgg ctcaccgaaa
      721 agggcatttg cctgttcgcg gtaatcagga agtgacggag agatgggata gaagaacatg
      781 tctccaccgt actccgtcag gtcaggtcca acgtagttgt atggaataat ttctgctgca
      841 aggacgtgtt tacgcagaat atcaaaaagg ttttcacgat ctgtgagacg ctttaatgga
      901 tagtaaagcg ggaagttaat cttctcacgt gtatcaatta cacgccagat acgcttttcc
      961 cacatcacat ccgcttcacg aagatgcgga tactgaatca gcttcttggt gggaatgtgc
     1021 tccttcacat ataccccatc aaggacgttc tgtgcgctca ccggagtacc gagagcaacc
     1081 agcgccataa ctatgagcaa ataccttttc atatttctgg tttttatttc cttttgcaat
     1141 tattacttca ctttgaacac cattggtggt acacgcactc ctcctgcggg accttgtgcc
     1201 gagattgaat tgaatgcaat aactgtaccc ggcttagcca acttgataaa gttcaacata
     1261 tcaggagtaa gctgtccacc cttagcaggg aacgacttac ttgctgcccc aacagttgca
     1321 gtcatttcaa atgaattcac tgtaaacttc actttagcag ggtcaagaga gatacttgga
     1381 tcgtaagcaa cacgcaggaa ctgcgcattc gtcttcaatt ctccaagtga tacatctgat
     1441 tgcaccgaac ttaggttacc caggtaagaa cttgggatag gcaatggctt aacagtaaat
     1501 tctggtccgg caaaagattt tgaacctcca ccatctgttt ttactgtaac tcctgctttg
     1561 atctttttgt tgatcaagct tgccggagcc ttaaccgaga atttccctcc acctttaggt
     1621 gaaactgaaa taccgggcag tgaaagcgaa acgcgatcct gtggataacc ggatactgaa
     1681 gcagaaaacg tatgctcgta acctgcgtaa ataaccgtaa gatcttcgtt ggcaattaca
     1741 ccggttggtt caccaacttc gtacatgtat ttccaaggct tccacttttc ttctccgttt
     1801 tctttaactg ataccaaacc atacatggta taagcccccg gacgacttcc ttgaacagaa
     1861 agcttgccaa cgtgatcctt atagtttgca ggattggaat cgttcacagc gaaacgaatt
     1921 tttgcaactt cattagagtc gtatgcagca atcattacgc taagattcat ggaatctcca
     1981 acgttcagat aacttgtacg agcaaatgca agtggttcaa ttttgttaaa gttgaactta
     2041 ggtgcattca cctttccaag aaggtgatcg accgctacag attctgcatt acgaacgtcg
     2101 ttacgcagtg aagtaaacaa cgcagcagca gctacaaccg gtgcgtggtc gaacatacct
     2161 gcctgccagg gaacttcctc gtcatgattc ttcatggttg caggaagagt cagcatgccg
     2221 tagagctgct tgatctttgc agtgtctttt gggttagccg ttcctaatgc agcattcaac
     2281 tcttcaggcg acattgaagc accatcgaat ttcggtgtaa acgtaaaatt gtttttaccc
     2341 gattcatagg tagaaatgat gtagcaaagt gaatcgcgga aattgtgcat ttcggtcatc
     2401 aacgctttac cagcttcaac aatattggaa cgatcaccta cgaataagcg agtagctgcg
     2461 tcgtagtcat ctttcgatcc aatatccatt aaattggcca gctttctttt tccaccttct
     2521 tcctgaataa cccattcctt agcatcacct tcaacagctt tgatcagttt attggtttca
     2581 tcaaggtaaa aatcatgtac acgattggcc aatttctcaa tctgttgagc tttcggtctc
     2641 cagaccttaa ccaattctct ggtttgcggt acgttgtagt agttttcaaa atcctgatag
     2701 ccgaccgcgt ttttatactc gatcgctgca gtagattggt ccagtttatc gtttagcgta
     2761 atgaacgcta caacaatttc cttggatacg ttaagtgcca gaagtgcggt gagtaccaag
     2821 tacatcatac cgatcatctt ctgacggggt gtttctttac ctccagccat agtttttagt
     2881 ttttagggaa tctcctgctc tcatcacaaa aagtgataag cttgggaaat ttacccgcga
     2941 cttccggtat taatgttcat tgctgcaagc atgttgccgt aaattgtatt caaggcagtc
     3001 aggttgcgtg caaggtcaga catgttctct ttatacttct tggtatcttc aagtgaatcg
     3061 tgcaggttct gcatgagttc attgattcca ccgtaaagct tagatgtagc ttcgaggtga
     3121 ctagaagaac ctttgagctg catttcgtaa atattgttca acgcagaaag gttagttgac
     3181 aatttctgca tctgttctcc gaaagaagca ccttcagatt gcgtgttggt aagacccatg
     3241 atcgattcag aagccttaac ataagaatct gaaagctgtt caactttctt ggaagctcca
     3301 cgaagactgt cgatatattc atttgttgca actgatgcgt tcgatacatc accaattttg
     3361 tgggttgcat cggtaagtga acgcaaaccg ttaccaagac tctgaatgag ttcaggttcg
     3421 attttcgctt cttcaagcat cttgtctaat tcttcagtaa ctgaaccttt tttaccgtga
     3481 tgctcgatag caccgtgacc atcatggtca tccatatgcc caagagcaag ctcagggtaa
     3541 acaagttccc acttcggatc ttcgtgaatt ggttcgaagg ccgagaaaat aaagatgacc
     3601 gcttcagtcg aaagaccgat aacgagcatc gctcctgcac cgggccagtg cataatttta
     3661 aacatcgccc cgacaattac aaccgctgct ccgaatccgt acagcttggc cataaacact
     3721 ttccacgctt tgcttcctgg tttcatagtt ctcttgcttt atttggtttt acattgtttg
     3781 attgcttttc cgaaaattgt tttgaccatt aacgagcttc ctcagcctgg tcaggtccac
     3841 cacgacccaa ataggtcatt acacagcggt atcctacata tgatttagca gaatcctggt
     3901 attcgaatga acgactacct gtctgtaaat agtaagcgat atccttccat gaacccccac
     3961 gaatcacttt acgcttttgc gctggtgggt catggtcaag tgcatcgtaa cgacgctctg
     4021 gattcaagtc gtgaatgatc tcatagttcg attcatcgta ggctgtttcg caccactcag
     4081 atacgtttcc tgccatacaa tacagaccga agtcgttagg atggtaagac gtaactttta
     4141 ctgtgtggaa tccaccatct tccatgtaac gtccgcgcat cggcttaaag ttaccaagga
     4201 aacatccgcg tgcattacgg atataaggac caccccaagg gtatgggctt aaatcaagac
     4261 caccgcgtgc accgtattcc cattctgctt ctgtaggcaa acggaaatcc tgtacgtaga
     4321 attctcccat cgactccaac cagaagttga gcaattgagt acgccaaaca cagaacgctt
     4381 ttgcctgaac ccatgttact cccaccaccg gatactcatc ataagccgga tgccagaaat
     4441 acatgttggt cattgggtca ttgaacgagt aggtgaagtc actgatccaa caaagtgtat
     4501 caggataaac gtttatgatt tccttaatga tgaaacgtga acgatcggta tggccacgaa
     4561 tagcgttatt ctgccccttt ttgtttctat aacctaaatc caagtcacgt ccaacaggat
     4621 cttttgtgat ggggtcttca gggttagata aggtacggtg ttccggatcc tcaggatttg
     4681 ttttgatgtt gtaacgacct tttttagcag ctgcctgcag gtcgatccaa taatactcga
     4741 acatgagttt acgcacatca aattccttac gacgataata acgttcgtgg tcgggaaggt
     4801 acatttccga taacaaaggc atataatcgg ggtcgaagta ctttaagcgg gcagaccagt
     4861 taagatccca ctcagaagca tcgagctcat ttccttcgtc atcataacgc ggcatcagga
     4921 attcctcggg gaattcttct gccaatttac ggcgagcaat agagtcgcga acatagtaca
     4981 cgaattgacg gtactcgtta ttcgaaattt ctgtttgatc catataaaag gcctgaacag
     5041 aaaccgtttt tgaacgattc gtgtgcgccc agggcacatc ctgatcactg gctcccatat
     5101 tatagctacc agcgggaata tagagcatcc catacggggt gttctgatac caactcgggc
     5161 ggggaataac ccctaccagt tcaccgttgt tcccagggga acaggcaaac aatgtcacag
     5221 caattgctga atagaacaac aactttttca tgctacctcc ttaaattgtt tctccgggtt
     5281 tcgcaacctc ctgaatcgga aagcccgtga atgcctttaa cgctttcaat tgtgcgattg
     5341 ttacaattat ttataaaaat ctgactgtct ttgacttagt aatcttaacc ggtggtttta
     5401 ggttaaagca atagctcaac atgatctcat ggctaccact gctataatca cgaatctcac
     5461 tagtggttac atcataagag taaccaattc tgatcatccc cggcatcttg tcgttcccta
     5521 aaggctgctg aaaacctaac ataggtacaa ttgcatcctt gatacggtaa ctcaaacctc
     5581 cccaaaccat gttcttccaa agcacattag cattgaaatc aagctgagtg gaagtgaaat
     5641 cagattccaa acggaaggaa ggacgaatca tgatatcagg attgcttggc aactggaaat
     5701 catatcctcc caaaacataa tagtgacgtg cgttctttac attcaggttt acaagatccg
     5761 actccgttaa gtgagttgca gaaaggcctg cataccaatt ggttgattta taatacagtc
     5821 caaaagcaag gtcataggtc gattgtgtct gacttccgtc gggaatcgag ttgtccagat
     5881 taacgggatc gatagcaatc caatcaggct taagagaaac tttcatcaat cccgcagaaa
     5941 ttccaatccc taagatatca ccattaccga tactcaagtg atatgaataa ttcaagcgag
     6001 cagaaaaatt attgaagaat ccaatcttat cgctaaagag cgataaacct acaccacctc
     6061 ttaatagggg tacgggacca tgaatgttca ttagaaaagt ttttggcgct ccatcgaaac
     6121 cagaccactg gctgcggaag aaactggttg cgcagatctt gttgtcaatc ccagtgtaac
     6181 cagggttgat cgacaaacga tcatacataa actgagacaa ttgataatcc tgctgaccat
     6241 aggcaacaga acccattaca atcgctccca tagagagtaa aaacttcctc atataaagta
     6301 gatgttactt tatttacacg gtttagattt ggatggaata ctccattcaa agtccggtaa
     6361 agttaagtaa aaattattcc cgaaaaaaaa tttcgcaaca ttttcacaaa attttgaaca
     6421 atcttttaag aatcaggaaa gtttttgata agtcctccac caaaggtcag gcatctcacc
     6481 ctcagaggct tgcagataat cctgataaga acagggcacc atatgatgcc tttcgaactt
     6541 caaacgcttg tccgggggat agggcacatc catccaccaa cggtctgatc tgttgctctt
     6601 aaagaataaa atttcgtggt cgaactgcgg actggtcacc ctgtatttag taaacagtga
     6661 gcggtcgcca atcgggaaat ccttcttacg gttgtaatac ccatcaacca ggtaccatat
     6721 catctgggca acaagatggg tggtttgtcc atgactatcc cgtgcagggt ttaattcata
     6781 aaatcctgct gaggtcagtt tatcactcat ccctgcatat ctgcaaagct gacaagcctc
     6841 ctcaccgaat agcccattag gtcccgactc agcattgccg ggtgcgtcgg catggcgaat
     6901 cgccgaaaga tcgaaggaca agatatctgc attgcgaatc aaaggttcac aggcggtaat
     6961 atcctttcgc agttcgccca gtcggtaatt atcgaaatac aacttatcca ttaactccaa
     7021 tgatcttgcg tcggcgaaat gggactggag cgcaacatta caataattga aaaggtaatt
     7081 gggctggtgc atgataatct tccctaaaaa ggttcgggag gaaatttgat cttcaacatc
     7141 acccaaattg attctggaat caatggttac catgttaatg gtctgctcca gtttttcgta
     7201 agcgagataa ttggcaaaag tgatatcctg actacctcca ataatgattg gcaaaatatt
     7261 cctcttgatg agaaatgcaa ctgattggga aacggcgtac caggtatctt caattgttgc
     7321 tcctgctttt atatttccaa gatcagcaat tttgtaatca tgtccggccg gcgagagttc
     7381 atagagaaac ttacggactt catcaggagc ctttgagcaa cccttattgg cataagaatt
     7441 tctctcttcc attacaccga taatggcaat atccatcccg tttaaatccg gaaaatctcc
     7501 actccgatgg aaaacagtat gagcagacaa agtgccctga attatgcttt cagaacgaaa
     7561 ctccggaata aaacccggct caaaaaatat tgagatgtct ttcattctat tttacctgcg
     7621 tgtagttttt tttctgacag cagtggtttt cttttttgca gcagcagttg ttttacttcc
     7681 tgtggttttc ttcgcaacag acgtctttgc gggtgccttt ttcgcaggtg ccttttttac
     7741 agaagccgtt ccggttttct ttcttggtgc acttgttttc ttttttgctg caggacgttt
     7801 ggatgctgcc ttttttaaag taactccatc actttttcct tttttcaact ctttaactcc
     7861 tgcatcacct gctaattcca tgcacttttc aagcgtaagt ttttccggct ctagatcttt
     7921 gggaatacgg aagtttttct ttccgatttt caaaaacgct ccccaacgac cttttaaaac
     7981 ctgtatttca ctattctcag ggaatgcttt gatgattcga tctgcattag cctgacgttt
     8041 atctgccagt aaaacttttg ctctgtcgag agtgatcgtc attggatcat ctccatccgt
     8101 ctttttgatt gaagcaaaca caccatcgtg agcaacataa ggaccaaatc gtccaacatt
     8161 ggctacgacc ttcttgccat caatcatacc gagatctctt ggcaacttaa ataaatcaag
     8221 cgcttctgcc aaggtgatcg ttgcaatact ttggtccttt cggagtgatg caaatcgtgg
     8281 tttctcttcg tcttccgcat ttccaatcat caccatcggt ccaaatcgtc cgattttcac
     8341 tacaattttg cgtttggaag ttggatctac ccccaattca cgttcaccgg ttgcgcggtc
     8401 tgaatgttcc agcgtttttt ctacatcctt gtgaaagggt ccatagaaac gtttgatcat
     8461 cgcattccat ttcaccattc cctgggcaat atcatcgaat tctctctcga tggttgcagt
     8521 aaaactgaaa tccaacacat cgccaaaatg tttaacgaga aaatcattaa cgaccattcc
     8581 tatatctgtg gggaatagtt ttgatttttc tgcgcctgta ttttccgact taatttcctc
     8641 ggtgatcttg ccctttttca gagaaagtaa atcataatca cgacgaactc cttctctatc
     8701 ctctttcact acatatccac gattctgaat agtggatata gtaggtgcat aagtggaagg
     8761 tcggccaata cctaattctt caagtttttt tacaaggctc gcttctgtat agcgtggagg
     8821 atggtgagta aaacgctggg ttccggtgat ttcggaaggt tcaagtgctt gaccaacttt
     8881 cattggtggc agcattgc
//
LOCUS       DAOMYG010000069         8810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2637_length_8810_cov_9.957738, whole genome shotgun sequence.
ACCESSION   DAOMYG010000069 DAOMYG010000000
VERSION     DAOMYG010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8810
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2637_length_8810_cov_9.957738"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1761
                     /gene="pckA"
                     /locus_tag="PK610_03535"
     CDS             148..1761
                     /gene="pckA"
                     /locus_tag="PK610_03535"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRJ35028.1"
                     /translation="MNEFGIKSKHSTLEELGLGKVANAYWNLTPSELVEETIVHGQGV
                     LSDTGALAIETGEFTGRSPKDRFIVCDSKTENTVWWGDINIKFTPEKFDALFNRMKAY
                     LANRDVYVRDAFACADPKYRMNLRVVTEFPWSNLFAYNMFLRPEQKELEDFKADWHIV
                     CAPGFQADPAIDGTRQHNFAVINFTRQMIIIGGTGYTGEIKKGIFSVLNYILPQEKNT
                     LSMHCSANIGAGGDTAVFFGLSGTGKTTLSADPHRGLIGDDEHGWADGSVFNFEGGCY
                     AKTIDLTEEKEPQIWKAIKFGALLENIGFKENTSSVDYLDSSITENTRVSYPIHHIEN
                     AVTPSAGGDPKNIFFLTADAFGVLPPISRLSPEQAMYHFISGYTAKVAGTEVGVTEPQ
                     TTFSACFGRAFLPLHPAKYAELLGKKMKEHNVRVWLINTGWSGGAYGVGERIKLRFTR
                     AMITAALNGSLENEEFETLPVFHLNVPKSCPDVPTEILNPRNTWSDKDAYDQTLNKLA
                     GQFVKNFEQYASGTNQDILNAAPKIHANA"
     gene            1909..5484
                     /locus_tag="PK610_03540"
     CDS             1909..5484
                     /locus_tag="PK610_03540"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ35029.1"
                     /translation="MNRLLFLISLMLLSSGLFAAAPSTQASGTTFTTRNSNSLSVGWT
                     RGNGGRCLVAVKLGTSTTTPPPSTVPNASQTPFSASTVFGSGSNLGNGNYVVYQGTGT
                     SVTITGLNPSTSYTVIVYEYNSTWQPLLGLYYNEYNTSYGVANLEGVYTLCTEPTTNA
                     TNLQFSAINYTSAGIAFTPGNGSNTLVSLEGAADGYQYASPVDGTSYTASTTYGSGSL
                     LTGDNRVVYNGTGTSFTVTNLQPAQTYRVRAFEFCGSGTGATWNYRVTGYTYADFTTL
                     NNPPTLNTISNRTICQNAGQQSVSLSGISDGSSAETQTVTITATSSNQTLIPNGNISV
                     SYTNPNSTGTLFFTAATGQSGTATITVTANDGFSATNTLVRTFTVTVNPIPAAAGAIT
                     GVNAACKNGSNYIFSVPTIANATTYNWTFPSGTVIVSGGTTNTVTVNFPSAMAQNSGT
                     ISVYGSNSFGCGNGTTSSKNITFDRAPTVAAAGADVVICSGTTQLQGNAASIGTGIWT
                     VVSGSASFNNASQNNTNVTGIAPGQSVTLQWAISNGVCPTSTDQVIVNYNPSAPQCLS
                     FADFFANNTTPCVGATVQFTDNSAGATSWSWNFGPNATPTTSILQNPTVVFNAAGPQT
                     ISLTIGGPNGPDSESRPGYITVSAAPGNASAIAGLTTVCAGETQVNYSINPIVGATNY
                     EWTLPAGATINSGDGTDAITVNFSGSASSGAIQVYGENQCGQGNSSTLNVTVNPLPQS
                     PGIISGDISVCQGENGVTYTIAAIPNATSVTWGVPSGAIINGSSTTQVLVDYPVSSVS
                     GDITIYGTNTCGDGPASTLSVTVAPLPDPAGPITGLNLISFCPPSGNQAYQITPVANA
                     STYDWTLPTGVSIVSGTGTATVTLAFANNAISGDIIVTPQNACGSNGPSALTLVVDQL
                     PAQDVCVVSVDETSQHNIVVWQKPIAFDIDSFYVYREIAGLGFTKIAAVHYDSLSQYL
                     DTDAGINPNATQYRYKVSALDTCGNEGPQSTFHQTIYQFPPQVAGNDIILDWEDYVGL
                     PAGFYYRILRDSANTDSWSAIDSVPSGITVYTDLNGLLEGPSIRYIIEIVIPNSCVAT
                     RAQNHNSTRSNRTQPIVASVEENSLLALVQLVPNPASDLTRISIAGSVSPMQVDVYDI
                     NGRLITSERWNTSSGELNISTFEAGVYVVRVMVNGQSAHLRLVKQ"
     gene            5543..5779
                     /gene="rpmB"
                     /locus_tag="PK610_03545"
     CDS             5543..5779
                     /gene="rpmB"
                     /locus_tag="PK610_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRJ35030.1"
                     /translation="MARVCQITGKKMMVGNNVSHSNRKTKRKFFPNLLVKKFFVEEEN
                     RWVELKVSANGLRTISKKGITACLKEAREKGFID"
     gene            5844..7280
                     /locus_tag="PK610_03550"
     CDS             5844..7280
                     /locus_tag="PK610_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HRJ35031.1"
                     /translation="MKYILILFLVLAMNPILHAQEHKHLTIKGDSSLYDQDMLPASFH
                     KQRREEVRKLMPDSSIAVFFAAPIRNRSNDVDYEYHQDPNFYYLSGFNESNAVLVITK
                     TPVRFGSVITNEIIFVQDKDPGSEVWTGRRLGADQAKNVLEIDVAFQSSAFGEYPFNF
                     GRFSKVLIVPQHGDVRDDKNDRGDLFSMIKMFNDQVDPLKGKVNRYKLPEIMATLRQI
                     KTAEEIILLRKAIDITCQAQMELMKTLRPGMKEYQTEAIVEFVFATSGAESEGFPSIQ
                     GGGSNGCILHYNTNRKTLTSKDLLVSDIGAEYHGYTADVTRTLPVNGRFNKEQAIIYD
                     IVLRAQDAGIAKCKIGNKFWDPHEAATEVITKGLLELGIIKKAGEVKKYFLHGTSHYL
                     GLDVHDPGLYGTLQANMVITVEPGIYIPEGSDCDPKWWNIGIRIEDDILITSKGPENL
                     SACVPRTMAEIERIMAQSSLFEQFIPGK"
     gene            7352..7534
                     /gene="rpmG"
                     /locus_tag="PK610_03555"
     CDS             7352..7534
                     /gene="rpmG"
                     /locus_tag="PK610_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRJ35032.1"
                     /translation="MAKKGNRVQVILECTEHKTSGMPGTSRYVTTKNRKNTPDRMERK
                     KYNPILKKVTVHKEIK"
     gene            7542..7694
                     /locus_tag="PK610_03560"
     CDS             7542..7694
                     /locus_tag="PK610_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015024932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HRJ35033.1"
                     /translation="MAKKTVATLQKGGKNLTKVIKMVRSDKSGAYSFKEEIVPNEKVK
                     DFFAKN"
     gene            7760..8719
                     /gene="ftsY"
                     /locus_tag="PK610_03565"
     CDS             7760..8719
                     /gene="ftsY"
                     /locus_tag="PK610_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRJ35034.1"
                     /translation="MGIFSFFSKDKKESLDKGLEKTKDNFFSRISRAIVGKTTVDDDV
                     LDELEEILVSGDVGVDTTLKIIKRIEARVARDKYVNTSELNKILKEEVASLLEENNSK
                     GNDSEITIPAGKKPYVIMVVGVNGVGKTTTIGKLAWQFKSQGKSVLLGAADTFRAAAV
                     DQLKVWSERAGVPIVAQGMGADPAAVAFDTVQSGAAQNADVIIIDTAGRLHNKVNLMN
                     ELGKIKKVMQKVLPDAPHEVLLVLDASTGQNAISQCREFTNATQVTALALTKLDGTAK
                     GGVVIGISDQFRIPVKYIGVGEKMEDLQAFNKNEFVDSLFKGA"
BASE COUNT         2584 a         1816 c         1906 g         2504 t
ORIGIN      
        1 caggatttag cagttttaaa gttatcaacc gaaatgaggc tgttaataac ctctgttttc
       61 ggtggataat gcgcaagact ttgcaggata acgggaacaa ggaataatta actttgtgcc
      121 ctgaaaatga taactaatcc ttcatttatg aacgaattcg gaataaaatc caagcattct
      181 acactcgagg agcttggttt gggtaaagta gcgaatgctt actggaatct tacaccgtct
      241 gaactggttg aagaaaccat tgtacacgga caaggggtat tgtcggacac cggagcactt
      301 gcaattgaga cgggagagtt taccggtcgt tcacctaagg atcgcttcat tgtatgcgat
      361 tccaaaactg aaaacaccgt ttggtggggt gatattaaca tcaaatttac tcccgaaaaa
      421 ttcgatgcgc tctttaacag aatgaaagcc tatctggcca atcgtgatgt ttatgttcgc
      481 gatgcatttg cctgcgccga tcctaaatac agaatgaatc ttcgtgtggt tacagaattt
      541 ccatggtcga atctttttgc ctacaacatg tttcttcgtc ccgaacaaaa ggaactggaa
      601 gatttcaagg cagactggca tatagtatgc gctccgggtt ttcaggcaga tcctgctatt
      661 gatggtactc gtcagcataa ttttgcagta atcaatttta cccgtcagat gattatcatt
      721 ggtgggacag gatataccgg tgaaatcaaa aagggcattt tctctgtatt gaattatatc
      781 cttccccaag agaaaaacac cttatcgatg cactgttccg caaatattgg ggcaggtgga
      841 gatactgcgg ttttcttcgg tttatccggc accggaaaaa caactttatc cgcagatcct
      901 cacagaggat taataggtga tgatgaacat ggttgggctg atggttctgt tttcaatttt
      961 gaaggcggtt gctatgctaa aacaatcgac cttactgagg agaaagaacc acaaatctgg
     1021 aaggccatca agtttggtgc gcttttggaa aacatcggtt tcaaagagaa tacatcttct
     1081 gttgattacc tcgatagttc aattaccgag aatacccggg ttagctaccc gattcatcat
     1141 atcgaaaatg ctgtcactcc ttctgccggt ggcgatccta agaatatttt ctttttgact
     1201 gcagatgctt ttggagtgtt gcctcccatt tccagactat ctcccgagca ggcgatgtac
     1261 cactttattt ccggttatac tgccaaagtt gccggaacag aagtaggagt aacagaaccg
     1321 caaaccacat tctcagcatg tttcggtcgc gcgtttcttc ccttgcatcc tgccaagtac
     1381 gcggagctat tgggtaagaa aatgaaggaa cacaacgttc gtgtttggtt gatcaatacc
     1441 ggttggtcgg gtggtgcata tggtgttggt gaacgtatta agttacgctt tacccgcgca
     1501 atgattactg ctgcattgaa cggaagtctt gaaaacgagg agtttgagac gcttcctgtg
     1561 tttcatttga atgttccaaa atcatgccct gatgttccaa cagaaattct gaatccgaga
     1621 aatacctggt cagataagga cgcatatgac cagacactga ataaacttgc tggtcaattc
     1681 gttaagaatt tcgagcaata tgcttccgga acaaaccaag atatcttgaa cgcagcccca
     1741 aaaattcatg ctaacgcttg attattaggt ttttaatact gaattcaaaa ggactgatgc
     1801 acgtcagacc tttttttgtt caaaactacg ctgtcaatat tgacactttt accttggaat
     1861 gaggcaaata aaccgataaa ttcgttcaaa atttcgataa gtatgatttt gaaccgttta
     1921 cttttcctta taagtctgat gctgctctca tccggacttt ttgctgcagc tccttcaact
     1981 caggcttctg gtacaacatt tactaccaga aacagtaatt ctctaagcgt aggttggaca
     2041 agaggaaatg gtggacgttg cttagttgct gtaaagcttg gaacaagtac taccacccct
     2101 ccaccctcta ctgttccaaa tgccagtcaa accccatttt cagcaagcac tgtttttgga
     2161 tcaggtagta atctgggtaa tggaaattat gttgtttacc aaggcacagg gacttcggtt
     2221 acaattacag gccttaaccc ttctacaagt tatacggtta ttgtgtacga atacaattca
     2281 acatggcaac cattattagg tctttattac aacgaataca acacttcata tggtgttgcc
     2341 aatctggaag gggtatatac tctatgcaca gagccaacaa ccaatgcaac caacctacag
     2401 ttcagtgcta ttaactatac atctgcagga attgcattta caccgggcaa tggatcaaat
     2461 acgcttgttt cccttgaagg agctgccgat gggtatcagt atgcaagtcc tgttgacggt
     2521 acttcttata ccgctagtac aacttatggt tcgggatcac tattaaccgg agataatcgt
     2581 gtagtttata atggaacagg cacctcattt acggttacca atcttcaacc ggcacaaact
     2641 tacagagtgc gtgcttttga attttgtggt tcaggtaccg gagctacatg gaattaccgt
     2701 gtaacaggtt acacttatgc agattttaca acactcaata accctccaac cctcaatact
     2761 atttccaacc gtactatctg ccagaatgca ggtcaacaat cggtttctct ttccggtatt
     2821 tcggatggta gttcagctga aacacaaaca gtgacaatca ccgcaacttc cagtaatcaa
     2881 accttgattc cgaatggaaa tatttcagtt tcttatacaa atccaaattc tacaggaaca
     2941 ttattcttta ctgcagcaac aggacagtca ggaacagcaa ctattactgt aactgccaat
     3001 gatggatttt cggcaacaaa cacattggta agaactttta ctgtaactgt aaacccaatt
     3061 cctgctgcag ccggagctat taccggagtt aatgctgcct gtaaaaacgg atccaattat
     3121 attttctccg ttccaacaat tgccaacgca accacctata actggacatt cccatcggga
     3181 actgtgattg tttccggtgg tacaacaaat acagtaacag tgaattttcc ttctgcaatg
     3241 gcgcaaaatt caggaacaat ctctgtttat ggttcgaatt cattcggttg tggaaatggt
     3301 acaacaagtt ccaagaacat tacgttcgac agagctccaa ctgtggctgc agccggagct
     3361 gatgttgtca tttgttcggg tactacgcag ttgcagggaa atgcagccag tatcggaacc
     3421 ggaatctgga ccgttgtttc tggaagtgca tcatttaaca atgcatctca aaacaacacg
     3481 aacgtaaccg gtattgcacc gggtcaatct gtaaccttgc agtgggcgat ttcaaatggt
     3541 gtttgtccaa cttcaacaga ccaggtgatt gtaaattaca acccttctgc accacaatgt
     3601 ttgtcgttcg ccgatttctt tgccaataat acgactcctt gtgttggagc cactgttcag
     3661 tttacagata attcagcagg tgcaacaagt tggagctgga attttggtcc taatgcaacc
     3721 ccaactactt ctatacttca aaacccaact gttgttttca atgctgcagg tcctcaaacc
     3781 atttcactta caattggtgg tccaaatggt ccggatagtg aaagtcgtcc cggatacatt
     3841 acagtttctg cagctccagg aaatgccagc gcaattgccg gtttaactac agtatgtgca
     3901 ggcgaaacgc aggtgaatta ctctattaat ccaattgtag gagcaaccaa ctatgaatgg
     3961 acattacctg caggagcaac catcaattca ggtgatggta ctgatgcgat tactgtgaat
     4021 ttttcaggat cagcatcaag tggtgctatt caggtgtatg gagaaaatca gtgtggtcag
     4081 ggtaacagca gtacattgaa tgttacagta aacccattac cacaaagccc gggaattatt
     4141 tcgggtgaca ttagtgtttg tcagggcgag aatggagtta cctacaccat tgcagccatt
     4201 ccaaatgcaa cttcagtaac atggggcgtt ccttcaggag ctattatcaa tggatcatct
     4261 actactcagg ttttggtaga ttatcctgtt tcttcagttt ctggtgatat tacaatttat
     4321 ggaacgaaca catgtggcga cggacctgca tccacacttt cagttactgt tgctccattg
     4381 ccggatcctg caggaccaat tactggatta aatctaattt ccttctgtcc accttcagga
     4441 aatcaggcat accagatcac gccggttgca aatgcttcta cctacgattg gacacttcca
     4501 accggtgttt ctatcgtttc aggaacagga actgctaccg ttaccctggc attcgctaat
     4561 aatgcaattt caggagacat cattgtgact ccgcaaaatg catgtggatc aaatggtcca
     4621 tcagcattaa cgctggttgt tgatcagtta cctgcacaag atgtatgtgt agtatctgta
     4681 gatgaaactt cacagcacaa tattgtggta tggcagaaac caatcgcatt cgatatcgat
     4741 tctttctatg tatatcgcga aattgcaggt cttggattca ccaaaattgc ggcagttcat
     4801 tacgattcat tgagtcagta tctcgataca gatgcgggga ttaatccaaa tgcaacccag
     4861 taccgctaca aagtttcggc tttggatacc tgcggaaacg aaggcccaca gagtacgttc
     4921 caccagacca tatatcaatt ccctccgcaa gttgcaggaa atgatatcat ccttgactgg
     4981 gaagattatg ttggattgcc agctggtttt tactaccgaa tcctgcgtga ttctgccaat
     5041 accgattctt ggtcggcaat tgactctgtt ccatcaggta ttaccgttta cactgacctt
     5101 aacggactgc tcgaaggccc atcaatccgc tacatcattg aaattgttat tccaaattca
     5161 tgtgttgcta cccgtgcaca gaatcacaat tcaacccgct ctaaccgcac ccagccaatt
     5221 gttgcctctg ttgaagagaa cagtttgttg gcattggttc agcttgttcc aaaccctgca
     5281 agtgacctta cacgaatcag cattgccggt tctgtatcac ctatgcaggt tgatgtgtat
     5341 gatatcaatg gacgtttaat aacttctgaa aggtggaata cttcctccgg agagctgaat
     5401 atcagtacgt ttgaggctgg agtgtatgtg gttcgggtta tggtgaatgg acaatcggct
     5461 cacttaagac tggtaaaaca ataataattc ttgtttaatc aaaaattagc gttatttttg
     5521 cgctcctaaa aattgaatag caatggcacg agtttgtcag attaccggta agaaaatgat
     5581 ggttggaaac aatgtttcac actccaaccg caagacaaaa cgtaagtttt tccctaacct
     5641 tttggtgaag aaattctttg tagaagaaga gaatcgctgg gtagagctaa aagtatctgc
     5701 caatggtctt cgtaccatca gcaaaaaagg tatcacggct tgtttgaaag aagctcgtga
     5761 aaagggtttt attgattaag cacgattttt gtaatcataa gaaacgttgt cgtaacccgg
     5821 caacgttttt ttatttttag cccatgaagt acattctgat tctatttctg gttcttgcaa
     5881 tgaaccccat tctccacgca caggagcata aacatcttac tataaaaggg gatagttcac
     5941 tttatgatca ggacatgtta cctgcatcct ttcataaaca gcgtagggaa gaagttagaa
     6001 aactgatgcc cgatagttct attgcggttt tctttgcagc acccattcgt aatcgctcca
     6061 atgatgtcga ttacgaatac catcaggacc ccaatttcta ttatttgagc ggttttaacg
     6121 agtcaaatgc tgttttggta atcaccaaga caccggttcg gtttggatcg gttataacga
     6181 acgaaatcat ttttgtgcag gataaagatc ctggtagcga agtttggaca ggcagaaggt
     6241 tgggcgcaga ccaggctaaa aatgtacttg aaattgatgt tgcatttcaa agttctgcgt
     6301 ttggagaata tccattcaat ttcggaagat tttcaaaagt cttgattgtt ccgcaacatg
     6361 gggatgtaag ggatgataaa aacgataggg gagacttgtt ttcgatgatc aaaatgttca
     6421 atgatcaggt tgatcctctc aagggaaaag tgaatcggta taagctaccg gagatcatgg
     6481 ctactttgag gcaaataaag actgctgaag aaataatcct gcttcgaaaa gcaattgata
     6541 ttacgtgtca ggcccaaatg gaattgatga aaacacttcg tccgggtatg aaagaatacc
     6601 agacagaagc tattgttgaa tttgtttttg ccacatctgg tgcagaaagt gaaggatttc
     6661 cttctataca aggtggtggt tccaatggtt gcattcttca ttacaatacc aaccgaaaaa
     6721 ccttaaccag caaagatctt ctcgtgtcgg atattggtgc agaataccac ggttacactg
     6781 cggatgtaac acgtactcta cctgtaaatg gacgatttaa taaggagcaa gccataattt
     6841 atgatatcgt tttacgagct caggatgcag gtattgccaa atgcaaaatc ggaaataagt
     6901 tctgggatcc tcatgaagca gctacggagg tcataacgaa aggattgctt gaactgggaa
     6961 tcattaaaaa ggcgggtgag gtaaaaaaat atttcctgca tggtacttct cattaccttg
     7021 gacttgatgt tcatgatccg ggattgtatg ggacattgca ggccaatatg gtaataacag
     7081 tagaacccgg tatttacatt ccggaaggca gcgactgtga tcccaaatgg tggaacatcg
     7141 gaattcgtat cgaggatgac atcctgatta ccagtaaagg accggaaaac cttagtgcct
     7201 gtgtaccacg cacaatggct gaaattgagc ggataatggc gcagagtagt ttatttgagc
     7261 aattcatccc cggaaaataa ttattccagt tgcttggata aaagaaaaat gtatctttgc
     7321 agcccgtttg tatcaattat tcaattaagc aatggcaaag aaaggaaata gggttcaggt
     7381 gattcttgag tgtaccgagc acaagacttc gggtatgcct ggaacttctc gttatgtaac
     7441 aacgaagaac cgcaaaaata ccccggatcg catggagcgt aagaaataca atccgatcct
     7501 caagaaagta actgttcaca aagagattaa ataattaagc catggcaaag aaaaccgtag
     7561 ctaccctgca aaaaggcgga aagaacctca cgaaagtgat taaaatggtt cgttcagata
     7621 aatccggcgc ttactcattc aaggaagaaa ttgttcctaa tgagaaagtg aaagattttt
     7681 tcgcaaagaa ttgatctgtt atcaagtata actaaaggct tcttccgtcg gaggaggcct
     7741 tttcttgttt tcaaggaata tgggcatatt cagtttcttt tctaaagaca aaaaagagag
     7801 tctcgataaa ggtctcgaaa agaccaagga taattttttc agcagaattt ccagagctat
     7861 tgtcggcaaa acaactgtcg atgatgatgt gctcgacgaa ttggaggaaa ttcttgttag
     7921 cggcgatgta ggtgttgata cgaccttaaa aatcatcaag cgtatcgaag cgcgtgttgc
     7981 ccgcgataaa tacgttaata cttcagaact caacaaaatt ctcaaagagg aagtcgcgtc
     8041 tttacttgaa gaaaacaaca gtaaaggcaa cgattcagaa attacaattc ctgcgggcaa
     8101 gaaaccctac gtaataatgg tagttggtgt aaatggagtg gggaagacta ctaccattgg
     8161 taaactggcc tggcaattta aaagtcaggg taaaagtgtt ttgcttggtg ctgctgatac
     8221 ctttcgtgct gctgctgttg atcagcttaa agtttggtca gagcgtgcag gtgtacccat
     8281 tgttgcgcag ggaatgggag ctgatcctgc agctgttgca ttcgatacag ttcaatccgg
     8341 tgctgctcaa aatgccgacg tgatcatcat cgatacggca ggacgtttgc acaataaggt
     8401 aaaccttatg aatgagctgg gtaagatcaa aaaagttatg caaaaggtat taccggatgc
     8461 tccgcacgaa gtattactgg ttttggatgc ttctaccggt cagaatgcaa tcagtcaatg
     8521 ccgcgagttt accaatgcaa cacaggtaac tgcactggcg cttactaaac ttgatggtac
     8581 agccaaaggt ggagtagtta tcggtatttc agatcagttc cgtattccgg taaaatacat
     8641 tggcgttgga gaaaaaatgg aagatctcca ggcttttaac aagaatgaat ttgttgattc
     8701 cctgtttaaa ggcgcttgat cagaaatcaa agcctccaaa cattgattca aatcctccgt
     8761 atcccgacga tttagccctt tttctgcctt cggtgcgtat ttccttacgg
//
LOCUS       DAOMYG010000070         8806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2640_length_8806_cov_9.176551, whole genome shotgun sequence.
ACCESSION   DAOMYG010000070 DAOMYG010000000
VERSION     DAOMYG010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8806
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2640_length_8806_cov_9.176551"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            367..2148
                     /locus_tag="PK610_03570"
     CDS             367..2148
                     /locus_tag="PK610_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRJ35035.1"
                     /translation="MRRFQRLYLTIVTRRQEYIGNRAFTVLLAILVGLMAALAAMFLK
                     TLIHFIEEEGIEWLPPYFFFFFPMIGIFIVLGLYGRVFKNAAGFHGIGGVLEAVRRKS
                     SILHYSLMYAQMITAAITIGFGGSSGLESPTVVTGSAIGSNSSRFFRLNYKYRTLFIG
                     CGTAATIAAIFNAPIAGVIFATEVILPQFSATIFIPILIASATGAFFSELFLGNNVLF
                     RVEGVVPFTLKEIPLIIVLGIFAGFVSVYYTQVFALLKSALGKVKAKWRKAFIGGLLL
                     GSLIFLFPVLFGEGYIGIRQIISGEFGKIWQESVFGNYGEENYYTPLLVFGLLILLKP
                     IAASVTVHSGGEGGQFAPSFVTGGFTGFFFFLIASNFLPAGEVLHPTNYILLGMSAVL
                     AGVMHAPLTGIFLVAEVTESYDMFIGLMLVSAISFFTKIYFDKVPIHFKGSKSAAEMD
                     STKHEFISLNNIRVADLVERKFQSGTPETRLSEFGDVFAGNDSVTIAVRSGDELVGIV
                     TEHQLRKLLGRDVSLSSIKLGDIMALPATYIDLKDSMDMVVHKFDTYDVHYLPVLNKG
                     KFIGFISRMKVLSSFREALKSSGDILG"
     gene            2319..3953
                     /locus_tag="PK610_03575"
     CDS             2319..3953
                     /locus_tag="PK610_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HRJ35036.1"
                     /translation="MNRLLLVLLLILPYSAFSQEEGKALKTKIEKVTVYLNGAEIAHQ
                     GSVSLSEGTSVLLVKGLSPKVVKESIRAQFSNGVKVLAVGFEQDRITKGKQDSVRLTR
                     ISDTIRIMDREKRRINAMQSALEAEKQTIISNSNLVHGQNNVSVAELQRLSELYKTRM
                     SEIFRLIHLNQENLMDIERVIVRYNDETQKLKSVVKVEYDPQIRITVHAPGPVTAQLS
                     LKYLTGGAAWAPTYDIISEGSDKGIRLEYNANVMNQSGEDWKDVKITLSTTDPLQSHK
                     LPGMAPWYLNYGGGSEQQSQNRKVSKEEAQASYGDIKILDNVQYEEMIVSNISVDFPI
                     TGNFSIPSNSRLYQLEVARHDLSASFRYIAIPKIERNAFLVARLTGWEKLNLIQGPSN
                     IYFRGTYIGKAMIDPVTFDDTLDISLGRDNKVIVNRVKVEDKDAIKVIGSNKKETYTY
                     KISVRNTNTNPINIQLSDQIPVSQHSEITVDIHEVSNAEREDYSGKLTWNLDIKSGET
                     KEFIVSFSVKYPKNKTVKVQKSDKSNYRSVKTVRFL"
     gene            complement(4033..6489)
                     /locus_tag="PK610_03580"
     CDS             complement(4033..6489)
                     /locus_tag="PK610_03580"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HRJ35037.1"
                     /translation="MGYTTAFAQPDTLKVPQSQLDQEVNYSARDSMRFDIAKQRVYLY
                     GNAHVSYGNTNLTAGYIEFSMTDNHVYATGITDSTGKTTEKPHFTDGGEELTAAEIEY
                     NFETKKGVIREVRSRQGEGYVHMEVSKKHVNDEIHLEHGKYTTCELDHPHYYFNLSKA
                     IIIPDDKIVSGPVNLVVMDIPTPLALPFGFFPNQKRESKGILIPQYGESPVLGFFLLN
                     GGYYMPLGKKGKADLQLLGDIYSRGSWGAKTILRYNTKYKHNGNFNLSFTDLKRSDPE
                     FPDFSRNKEFFIRWQHNQDAKAHPYRRFSANVNAGTRNNFRNNFNTVSQDYLSNTFQS
                     NISWTYSIPNKPINLSANLRHNQNSLTGLVNLTLPELAMNVNRFFPFQGIRKTGSATK
                     KWYENIGMTYASNMRNDLTVQDTLLRSEYLPGVTSRMRNGMRHSGTISTSFKLFKQKF
                     TLNPAASFTERWYLQSIQKEWDNITQQPRTDTLRGFKRAGDYSLSASLTTKIYGMYSF
                     KGKRNAKLRHVITPSINLSYHPDFSTQQSGYFGPNGTLATYSPFDIGIYGKPPAGEAG
                     LISLNLVNNIEMKFKSARDTVTGYKKLVLIENFTLTGSYNVFADSINYSNINLSGRIT
                     LWRNLGLVYAGTIDPYRYVNGRKTGDLLWDNGSIGNFLGNNLALSWALRSKTKTAAKG
                     SLNTLNEEDRNEISRNQDAYIDFTIPWSLSINYNIRKDKLFSSLGTENRITQSLMLRG
                     DLSLTQNWKIGFTSGYDFTNKGLTYTEVNIFRDLHCWEMRFNWIPFGIRKSYMIQINI
                     KSSMLQDLRLMRRRSWFDAF"
     gene            6613..7896
                     /locus_tag="PK610_03585"
     CDS             6613..7896
                     /locus_tag="PK610_03585"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRJ35038.1"
                     /translation="MKRLLSMRLNERKRKVFLVLLLVFFQSASLFAAKDTLGIRTVVI
                     DPGHGGKDPGCLGKFSHEADVALAISLKLGKMIKTYYGDDVKVIYTRTEDKFVELAER
                     ARIANKVNADLFICIHANASANTGSKGTETYVLGLHKADAQLKVAERENNVILVEDNY
                     ETKYQNFNPKDPDSYIYLTMVASAFRTQSNRFAEHIQKYVAGTPNVTDRGVKQAGFLV
                     LHQVNMPSVLVETGFLTNPEEEKILNNEQDQFNIAKAIFKAFVDYKSEVDLRNGLPQK
                     DRGNPDEVITGKKNGQILKTDSVVIKDNKTNNPPDNSLDKTPEIKSPFMGSSQAPVSA
                     LPYSEEKVVFKVQIRTSSKEIGKDPANFYGLQGVEYYKADNLYKYTYGKSNNFDEAKK
                     WESEAREKGFKEAFVVAFLNGERIDINVARGMANK"
     gene            7904..>8806
                     /locus_tag="PK610_03590"
     CDS             7904..>8806
                     /locus_tag="PK610_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRJ35039.1"
                     /translation="MKISREAKIGAVVVVAAAFLIYGINYLKGIDLFKKGREFKVEYT
                     DLNGLVTAGPVSFQGMKVGQVTSIDLHRRETGEYYYEVTFVIDNNDLQFPDDSKARLF
                     SSDLFGTRAIDIIPGLSGTMAQEGLKLSSETEESMISRVDAQIAPIKKKAESLMGSLD
                     TMVTAVSHVVGENAEDLGQTIESMKLTIRNLKQITDKVNGLLESETGKVSSILTKFES
                     ISGNIQRNNESIDKSLDNVAAITDSLKNAGLASTVKQLSTTVTELNSILTKVNTGEGT
                     LGALINSDSLYLTLVQTNDQLNRLV"
BASE COUNT         2422 a         1742 c         2085 g         2557 t
ORIGIN      
        1 ttcgaacccc cgaccctctg cttgtaaggc agatgctctg aaccagctga gctaaactcc
       61 caatatttca aacactcatc cggataaacc agtcccttga aaagggatcc tgaccgaagc
      121 gtcaggactg agctaaactc ccaatatttc aaacactcat ccggataaac cagtctcttg
      181 aaaagagatc cagaccggcc tgtcccgacc gttagcgtcg gggaagcgtc tggactgagc
      241 taaactccct gaattgggag tgcaaatata gcggcttcgg caatacgtgc aagaaatcgg
      301 tgaaaaaaaa atcatcagcc ctcaaaatag caatcgctag ccttataaat agtaagtttg
      361 agcaacgtgc gacgctttca gcgattatat ctgaccatcg ttacccggcg acaagaatac
      421 attggtaacc gggcctttac tgtgttgctt gccattctgg taggactcat ggctgcactg
      481 gcggcaatgt ttctgaagac cctgattcac tttattgaag aagagggaat tgaatggttg
      541 cctccctatt ttttcttctt tttcccaatg atcgggattt tcattgtgtt gggattgtat
      601 ggccgtgtat ttaaaaatgc tgccggattt catggaatag gaggggtgct ggaggccgtt
      661 cgacgcaagt cctcgatcct gcattattcg ctgatgtacg cccaaatgat cactgccgcc
      721 attaccattg gttttggagg aagcagcgga ttggagtcac caacggtggt tacgggctct
      781 gcgatcggat cgaactcatc gcggtttttc aggttgaatt acaaatacag aacactgttt
      841 attggttgtg gtactgcagc taccattgcg gctatcttta atgcgcccat tgcaggagta
      901 atttttgcaa cagaagttat tcttccgcag ttttctgcca ctatttttat tcccattctg
      961 atcgcttcgg cgacgggtgc gtttttttcg gagttgtttc tggggaataa tgtactgttc
     1021 cgggttgaag gtgtagtacc atttacactt aaggaaattc cgctgatcat tgtgctggga
     1081 atttttgccg gatttgtatc ggtgtattac acgcaggttt ttgcgttgtt aaaatcggca
     1141 cttggcaagg taaaagcaaa atggcgcaag gcgtttattg gtggtttgct tttgggatcg
     1201 cttatctttt tgtttccagt attatttggc gaaggataca tcggcattcg tcaaattatt
     1261 tccggagaat tcggcaagat ctggcaggag agtgtattcg gtaattatgg tgaagaaaat
     1321 tattacacgc ccttactggt ttttggattg ctgattctgc tgaagccaat tgcagcatct
     1381 gttactgtgc attcgggagg agaaggcggt cagttcgctc cttcgtttgt taccggtgga
     1441 tttacaggtt tctttttctt tttgatcgca tctaactttt tgcctgcagg agaagtttta
     1501 catccaacca attatatttt gctgggaatg tcggctgtgc tcgccggggt gatgcacgca
     1561 ccgctgaccg ggatttttct tgttgcggaa gtcaccgaga gttacgacat gttcattgga
     1621 ttgatgttgg tgtcggcgat ttcatttttc accaaaattt attttgataa ggtgcccatt
     1681 cattttaaag gatctaaatc ggctgccgaa atggatagta ccaaacatga attcatttcg
     1741 ctgaataata ttcgtgttgc agacctggta gagcggaaat ttcaatccgg tactcccgaa
     1801 acacgtttaa gtgagtttgg tgatgtgttt gctggaaacg attcggtaac gatagccgtt
     1861 cgttcgggtg atgaactggt tggtattgta acagaacacc agttgcgaaa attattaggt
     1921 cgcgatgtca gcctatcatc gataaaactt ggtgatatca tggctcttcc tgctacttat
     1981 atcgatttaa aggatagtat ggatatggta gtacataaat tcgacactta tgatgtacac
     2041 taccttcctg tattgaacaa aggaaaattc attggtttta tttcgcggat gaaggtactt
     2101 tcttctttcc gtgaagcgct aaaatcgagc ggtgatattc tgggataatg cctgggatgc
     2161 attccgtttc gatggttttt tatgatttac tttgctattg tgtgcctatt tcgcttcatt
     2221 tcaatgaata aacacgcatt ttttttatct tttcagctct gttcaacctt ttatcgcttt
     2281 aggtgtacag agcttcaacc acaacataaa ccctgcttat gaacagattg cttttagtac
     2341 tgcttttgat tttaccttat tccgcatttt cacaggaaga aggaaaagcc ctcaaaacaa
     2401 aaattgaaaa agttaccgtt tacctcaatg gggctgagat tgctcaccag ggttctgtga
     2461 gtcttagtga aggtacaagc gttttattgg taaagggatt gtcgccaaaa gtggtgaaag
     2521 aaagtattcg tgcccagttt tcgaacgggg ttaaagtatt ggcagtagga tttgagcaag
     2581 accgaatcac caaaggcaaa caggattcgg tccgtcttac acgaatttca gataccatac
     2641 gtattatgga tcgggagaag cgcaggataa atgcgatgca atcggcactt gaagcggaga
     2701 aacaaaccat catttccaat tctaatcttg tgcacggaca aaacaatgtt tccgttgcag
     2761 agcttcagcg cttatcggag ttgtacaaaa cgagaatgag cgaaattttc cgattgattc
     2821 acctgaatca ggagaacctg atggatattg aacgtgtaat tgtaagatac aatgatgaaa
     2881 cgcaaaaact gaaaagcgta gttaaagtgg agtacgatcc tcagataaga attacggttc
     2941 acgctcccgg accggtaact gcacaactga gtttgaagta cctgaccggt ggagctgcat
     3001 gggcaccaac gtatgatatt atttccgaag gttcagataa gggaattcgt ttggagtata
     3061 atgccaatgt gatgaatcaa tcaggagaag actggaagga tgtaaaaatt accctgagta
     3121 ccaccgatcc gcttcaatcg cataagttgc cgggtatggc tccctggtat ttgaattacg
     3181 gaggtgggtc agaacaacag tcgcagaaca gaaaagttag taaggaagaa gcacaggcca
     3241 gttacggcga tattaaaatt cttgataatg ttcagtacga ggaaatgatt gtatccaata
     3301 tttcggttga ttttccaatt accggtaatt tttcgattcc ttccaatagc cgcctttacc
     3361 aactggaagt tgcgcgccac gatctttctg cttcatttcg ttatatcgca attccgaaaa
     3421 ttgaacgtaa tgcatttttg gtcgcacgtt taacaggatg ggaaaagctt aatctgattc
     3481 agggtccgag taatatctat ttccgcggaa cctatatcgg aaaagcgatg atagatcctg
     3541 taacttttga tgataccctt gatatctcat taggaagaga taataaggta attgtaaatc
     3601 gcgtaaaagt ggaggataaa gacgccataa aagtgattgg ttcaaataaa aaggaaactt
     3661 acacgtacaa aatttcagtg cgcaatacca ataccaatcc cattaacatt cagttaagcg
     3721 atcagattcc tgtttcgcag catagtgaaa ttaccgtgga tatacatgag gtttcgaacg
     3781 ccgaacgcga ggattattcg ggcaagctaa catggaatct cgacatcaaa tcaggtgaga
     3841 ccaaagaatt tattgtttct ttctctgtaa agtatccgaa aaacaaaacg gtgaaagttc
     3901 agaaaagcga taagtcgaat taccgaagcg taaaaacggt tcgttttctc taacgagtat
     3961 tagaagataa ttatataatt attcttccgc tcgcactgag cctttcgaag tgtccatctg
     4021 tggaacctgc aatcaaaacg catcaaacca actgcgtctg cgcatcagtc ggagatcctg
     4081 caacatcgat gatttgatat tgatctggat catgtagctt ttgcgtattc caaagggaat
     4141 ccaattgaaa cgcatctccc agcaatgcag gtcgcggaag atgtttactt cggtatacgt
     4201 taatccttta ttggtgaaat cgtaacctga ggtaaatccg atcttccagt tttgggtgag
     4261 tgaaagatct ccacgaagca tgagcgattg ggtaattctg ttttccgtgc ccagactgct
     4321 aaatagtttg tctttgcgga tattgtaatt aatactaagc gaccagggta tggtgaagtc
     4381 gatgtaagca tcctgatttc tggaaatttc gtttcggtcc tcttcgttca atgtgttcag
     4441 gcttcccttt gccgcggttt ttgttttgct gcgcaaggcc cacgataaag cgagattgtt
     4501 acccagaaaa tttccgatac tgccattgtc ccaaagcaga tcaccggtct ttcttccatt
     4561 tacatagcga tagggatcaa tggttcctgc gtacaccaaa ccaagatttc tccataaggt
     4621 gatacgcccg ctcagattaa tgttggagta attgatggaa tctgcaaata cattgtagga
     4681 tcctgttaac gtgaaattct caatcagcac aagttttttg tatccggtaa cggtgtcgcg
     4741 tgccgattta aatttcattt caatattgtt caccagattg agcgaaatca gtcccgcttc
     4801 tcctgccggt ggtttgccgt aaataccaat atcgaaaggg gaataggtag caagcgttcc
     4861 gttcggacca aaatatccgc tttgctgtgt gctgaaatcg ggatggtagg ataggttaat
     4921 ggagggagta atcacatgac gaagttttgc gtttcgtttt cctttgaagg aatacattcc
     4981 gtaaatcttc gtagtaagtg atgcagaaag tgagtaatcg ccggcccgtt tgaatccgcg
     5041 caaggtatcg gttcgtggtt gttgcgttat attgtcccat tctttttgaa tactctgcaa
     5101 ataccagcgt tcggtaaaag atgctgcagg attcaaggtg aatttttgtt tgaatagttt
     5161 aaacgatgtg gaaatagttc cgctatgacg catgccgttc cgcattcgtg aagttacacc
     5221 gggtaggtat tcgcttctca ataaggtgtc ctgaacagtg aggtcgttac gcatattgct
     5281 tgcataagtc attccaatat tctcgtacca tttttttgtt gcacttcctg ttttacgtat
     5341 gccctgaaaa ggaaaaaaac gatttacgtt catcgccagt tcgggtaagg taagatttac
     5401 cagtcccgtt agcgagtttt ggttatgacg taaattggcg gatagattaa tgggtttatt
     5461 tggaatggaa taggtccagg aaatatttga ctgaaaggta ttactcaggt aatcctgcga
     5521 tacggtattg aagttgttgc gaaaattatt tcgtgttcct gcatttacat ttgcactgaa
     5581 tcgtcggtag ggatgtgctt ttgcatcctg attgtgttgc cagcggataa agaattcttt
     5641 gttacgcgaa aaatccggga attcgggatc gctgcgtttg aggtcggtaa aggagaggtt
     5701 gaaattaccg ttgtgtttgt atttggtatt gtacctcaaa atcgttttcg ctccccagct
     5761 tccgcgcgaa taaatgtcgc ccagcaattg caaatcggct ttgccttttt ttccaagcgg
     5821 catgtaataa ccaccattga gcaaaaagaa tccgagtaca ggcgattctc cgtattgtgg
     5881 aattaaaatg cctttcgatt cccttttctg attggggaaa aatccgaagg gcaatgcaag
     5941 cggtgttgga atatccatca ccaccagatt taccggaccg gaaacgatct tgtcgtcggg
     6001 aatgataatg gctttgctaa ggttaaaata gtaatgcgga tgatcgagtt cgcaggtggt
     6061 gtatttgccg tgttcaaggt gtatttcgtc gttgacatgt tttttcgaaa cttccatgtg
     6121 gacgtaaccc tctccctgtc tgctgcgtac ttcccgtatc actcctttct tggtttcgaa
     6181 attatattcg atctcagcag cagtgagttc ttcgcctcca tcggtaaaat gaggtttttc
     6241 tgtggtctta ccggtgctgt cggttattcc cgttgcataa acgtgattgt cggtcatact
     6301 gaattcgata taaccggcag tcaggttcgt gtttccatag ctcacatggg cattaccgta
     6361 gaggtaaaca cgctgtttgg cgatatcgaa acgcatggaa tcgcgtgcgc tgtaattgac
     6421 ttcctgatcg agttgcgatt ggggcacttt cagggtgtcg ggttgcgcaa aggcagttgt
     6481 gtaacccaaa aacagggtta tgaacaggta aattggggat aatactatgg caggactcct
     6541 gttcagcaaa aggtgtggta ctaattttgt tggtaagggt ggtcaaaact acacattaaa
     6601 aagctctgtt gaatgaaaag gctattgtcc atgaggttga atgaacgcaa gaggaaagta
     6661 tttcttgtgc tgttgcttgt gttttttcag tctgcatcgc tttttgctgc gaaagacacg
     6721 ctgggcattc gtacggtagt tatcgatccc ggtcacggcg gcaaggatcc cggttgtttg
     6781 gggaaatttt cgcacgaggc agatgtagcg ctggcgatat cgctgaagct gggtaaaatg
     6841 atcaaaacct attatggcga tgatgtaaag gtgatctaca cccgtaccga agataaattt
     6901 gtggaactgg cagaacgggc gcgtattgcc aataaggtca atgccgatct tttcatttgt
     6961 attcatgcca acgcctcagc caataccggt tccaaaggga cagaaaccta tgtgttggga
     7021 ctgcacaagg cggatgccca gttgaaagta gccgagcgcg agaacaacgt aattcttgtg
     7081 gaggacaatt acgaaaccaa gtaccagaat tttaatccca aggatccgga ttcctacatc
     7141 tacctcacaa tggtagccag tgcttttcga acgcagagca accggtttgc ggaacacatc
     7201 caaaagtatg ttgccggcac accaaatgtt accgaccgcg gtgtaaagca agcgggattt
     7261 ctggtattgc atcaggtaaa catgcccagc gtattggttg aaacaggttt tttgaccaat
     7321 cccgaagaag aaaaaattct gaacaacgaa caggatcagt tcaatatcgc aaaagctatt
     7381 tttaaggcct ttgtggatta taaatcggag gtggatttgc gcaacggatt acctcagaaa
     7441 gacagaggaa atccggatga ggtgataacc ggaaagaaga atggtcagat cctaaagacc
     7501 gacagtgttg tgatcaagga caataagacc aataacccac ctgataattc ccttgataaa
     7561 accccggaga taaagtcccc gtttatgggc tcttctcagg caccggtttc tgctttgcct
     7621 tacagcgaag aaaaggtggt ttttaaggta caaatccgaa ccagttccaa agaaatcggc
     7681 aaggatcctg ctaactttta tggtttacag ggagttgaat attacaaagc ggacaatctt
     7741 tacaagtata cttacggaaa aagtaataat tttgacgagg ccaagaagtg ggagagcgaa
     7801 gcgcgcgaga aggggtttaa ggaggctttt gtagtcgcct tcctcaacgg agaacgcatc
     7861 gatatcaatg tagccagggg aatggccaat aaatgacctc gttttgaaaa tcagtagaga
     7921 agcaaaaatc ggagcagtag ttgttgttgc agctgcattc ctcatttacg gaatcaatta
     7981 cctgaagggg attgacctgt ttaaaaaagg gcgtgaattc aaggttgaat acaccgacct
     8041 taacggactt gtaaccgccg gtccggtatc ctttcaggga atgaaagttg ggcaggtgac
     8101 cagtatcgac cttcaccgcc gtgaaaccgg agagtattat tacgaagtca cttttgtgat
     8161 tgacaataac gatcttcagt ttcctgacga cagcaaggct cgcttgttct cttcggatct
     8221 ttttggtacc cgtgctatcg atattattcc cggattatcc ggaaccatgg cacaggaagg
     8281 tttaaagctt tcatcggaaa cagaagagtc gatgatttcg agagttgatg cacagatcgc
     8341 accgatcaag aaaaaagcgg aaagtttgat gggttcattg gacaccatgg ttaccgccgt
     8401 ttcgcacgtg gttggtgaaa atgctgagga tcttggtcag accatcgaaa gcatgaagct
     8461 taccattcgt aatttgaaac aaattaccga taaggtgaat ggattgcttg aatcagaaac
     8521 aggaaaagtt tccagtatac tcaccaagtt cgaatcgatt tccggaaata ttcagcgtaa
     8581 caatgagtcg atcgataaat cgctggataa cgttgctgcc attaccgata gtttaaaaaa
     8641 tgcgggtctt gccagcactg tgaagcaact cagtacaact gtaacagaac ttaattcgat
     8701 tctcaccaaa gtgaataccg gagaaggaac acttggtgcg ctcatcaata gcgatagtct
     8761 ttatttgaca ttggttcaaa ccaatgatca gttgaaccgt ttggtt
//
LOCUS       DAOMYG010000071         8685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2696_length_8685_cov_9.674276, whole genome shotgun sequence.
ACCESSION   DAOMYG010000071 DAOMYG010000000
VERSION     DAOMYG010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8685
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2696_length_8685_cov_9.674276"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2893
                     /gene="dnaE"
                     /locus_tag="PK610_03595"
     CDS             <1..2893
                     /gene="dnaE"
                     /locus_tag="PK610_03595"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRJ35040.1"
                     /translation="SVEQLVEEAVAHGVKEMVLTDINNTSAAFDFVKECQEKGIRPAL
                     GIEFRDEEQKILYTGLAKNNKGYEELCRLLSTRLQDEQQLPDRAPESEHCFFIYPMEK
                     IAVKQLRKNEFIGVCHRQINKLIQLPFREQSHKMVVWQPVSIKADKHHYHLHRLLRSI
                     DKNILISKLKKEEHAAEDEHFISEKDLREKFILFPEINSNTQKIIEQCSIQLEYKTVK
                     NRKHFTSTEKDDRELLHKLAKEGLEYRYGKKNTRARERVEKELRIIEELDFSAYFLIT
                     HDIIHYARSRGFHHVGRGSGANSIVAYCMGITDVCPIELDLYFERFINPHRSSPPDFD
                     IDFSWDERDIIIDYIFKRYGKQYTAQIATYTTFKGSSIIRELGKVFGLPKEEIDGLAN
                     HPDKIEQRDNITKAIFHYGKMMENFPNYLGIHAGGIIISEEPLYNYTALQMMPKGFPI
                     VHWDMYVAEDIGFYKYDILSQRGLGHIKDAVDIVQKNRKINIDIHDVKNFKTDSKVRN
                     QIRSANTIGCFYIESPAMRQLLTKLRCDNYLTLVAASSIIRPGVASSGMMKEYIQRFH
                     HPKGFQYLHPKMEELLQETYGVMVYQEDVIKVAHHFAGIDLADADILRRAMSGKYRGK
                     GEMERITETFFRNCRERGYPEAIAKEVWRQIESFSGYSFSKAHSASFAVESYQSLYLK
                     TYFPLEFMVGVINNFGGFYRTELYVHEARMAGASIEAPCVNKSHYVTSITGTTIWLGF
                     VHMQHLARNTVNMLLENRHESGAFESLEDLVARLPITLDQLVLLIRMGSLRFTGKSKQ
                     ELLWQAHFLFNGKKRSAPSNDLFKAEVRKFDLPAFDEHPLQKAYDEIELLGFPLCGWF
                     DLLKTKFRGDIMAHEMLQHCGESIRMLGYLVTTKPVRTKHGDLMYFGCFIDYKGQFFD
                     TVHFPDTLERYRFKGNGIYLMRGTVADEFGSASLQVEKMEKMEYVPIQN"
     gene            complement(2906..3394)
                     /locus_tag="PK610_03600"
     CDS             complement(2906..3394)
                     /locus_tag="PK610_03600"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ35041.1"
                     /translation="MINWRKCSPNDISVIAALAQKIWRVHYPPIIGTEQVEYMLNKMY
                     SSDALANQFQEGQVFYLILEDELPVGFVAVSLKEVHHLFLHKFYIDDSMQGKGVGARV
                     FQFILDAYPQITTVRLTVNRQNYRSINFYFKLGFRIDQVADFDIGDGYVMNDFVMVYC
                     RP"
     gene            3541..4215
                     /locus_tag="PK610_03605"
     CDS             3541..4215
                     /locus_tag="PK610_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HRJ35042.1"
                     /translation="MAKFNDPTQHRPKYRENGFQFPKCAVITMKDPASIDHIRWGLIP
                     SWTRSRQDAFEIRIKTLNARAESIFEKPSFKTAILSKRCLVPATGYFEWMHEGKKKTP
                     YFISLNDAPLFSMGGIHESWIDPESGKAIETFSIVTTEANLLTGKIHNSKQRMPLILE
                     RADEEKWLDNKLSHSELEKLMRPHADQNMQAWTISDKLLQNAEDGDPKIIEEWVDPAG
                     LQGSLF"
     gene            complement(4226..6349)
                     /locus_tag="PK610_03610"
     CDS             complement(4226..6349)
                     /locus_tag="PK610_03610"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ35043.1"
                     /translation="MLRFLLLCFSLCVLSVNANAGFDIDSMRKATASVKPDTSRTLAY
                     FYQMKAIRRSGDYQTAIDLGYVGLEECSLVKDYHATGYIWGELGLSYDYLNQADSCKK
                     YFSKAFFYFARVERWDNVIGIFTNLGVHFLINEDYSNALYYMLAGLKVAERLNKPQYI
                     NVLSLNIGMIYFKLRDYEHARPYFIKTKESSEKSGDLVYQKYAEGNLAAVYFYTDNFD
                     SAYYYYTGVLSFYKMIGDKVELASFSNNMGAIHRKQNNLDSAEYYYNFSLALRKELGQ
                     EPTVEELNNLAILYEQKGEFRKAEELLNKALSINNPNFSRSQRESSYRILFQVLEKQG
                     KYKDAVHIGKKYLEIKDSIYKSSRLSEISDLRTQYEVEKNEDRVRKEEALEKEKLNAI
                     SREERKRRNLIIVFLSTGVLFVGLFSFFLFQRFRITKRQNEIIEKQRLEVTRQKHLVE
                     EKNKEVMDSITYAKRLQSAILPPVSAYSDHFRDSFVLYQPKDIVAGDFFFLDTVGPIV
                     IFAVADCTGHGVPGAMVSVVCSNALSRSVNEFGLTNPGKILDKVTDLVLETFSKGGEE
                     VRDGMDISLGSYNSETKELFWAGANNGLVIIRKNEITELKPDKQPVGKYLERRNFTAQ
                     RIVLQADDCLYLFSDGYGDQFGGELSSGKTGGKKFKEGNLKKLLLDICTIPMNQQEKL
                     LWNRLVEWRGELEQIDDICIMGLRI"
     gene            complement(6373..7128)
                     /locus_tag="PK610_03615"
     CDS             complement(6373..7128)
                     /locus_tag="PK610_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006265468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HRJ35044.1"
                     /translation="MFSGDIKQFMDEMTDRFERTDFIEADPISIPHQFNLKEDIEISG
                     FLSASIAWGQRITIVKNAGRMMQIMGNSPYDFVMDANPSQLERCSAFVHRTFNGSDFR
                     GVLLALRKIYSEDGGLEGVLSGEGTAFERIALLHRIFEMHLQERRTLKHISDPVSGSA
                     AKRLNMFLRWMVRSNKRKVDFGIWKNTSAADLICPLDVHTGNVGRKLGLLQRTQNDWL
                     AAEELTQSLREFDALDPVKYDFALFGLGVIDHF"
     gene            complement(7130..7783)
                     /locus_tag="PK610_03620"
     CDS             complement(7130..7783)
                     /locus_tag="PK610_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ35045.1"
                     /translation="MISIRSIEKSYGALKVLKGIDLDIAAGEIISIVGSSGAGKSTLL
                     HILGTLDVPDKGTYLFNEQDIAGMNNAGLASFRNKNIGFVFQFHQLLPEFNALENVCM
                     PALIAGNSLRDAEPRAVELLDYLGLKDRMHHKPSELSGGEQQRVAVARALMNKPSLVL
                     ADEPSGNLDSVNARELHKLFFELRDKFNQTFVIVTHNEELANMADRKLTIRDGVIVP"
     gene            7897..>8685
                     /locus_tag="PK610_03625"
     CDS             7897..>8685
                     /locus_tag="PK610_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRJ35046.1"
                     /translation="MNPSLVITILLSYFALLVTISWFTSRKADADSYFLGNKKSPWLA
                     VAFGMLGDSLSGVTFISVPGAVYTGNFAYLQLVFGYFAGYFVISEILLPLYYRLNVTS
                     IYSYLGIRIGSYSERTGSVYFLLSRLLGAGGRLYLAAGVIHLFVLQQLNIPFAVSVAV
                     IILLMLVYTLKGGIKTLVWTDVLQSAVLLLAVNLSILAIMHSTGKGIGEMISGIWSDP
                     HTETFFWDWKLKSFFPKMFIGGMLTAIAMTGLDQNMMQKNLSCKS"
BASE COUNT         2653 a         1992 c         1755 g         2285 t
ORIGIN      
        1 atcggtagaa caactggtgg aggaagcagt ggcgcatggt gtgaaagaaa tggtgctcac
       61 cgacatcaac aatacttcgg cggcgtttga ttttgtgaag gagtgtcagg aaaaaggtat
      121 tcgtcctgca ctgggtatcg agtttcgtga tgaggagcaa aaaatactgt atacgggtct
      181 tgcaaaaaac aacaaaggat atgaagagct atgccggcta ttgagcacac gactgcagga
      241 cgaacaacaa cttccggacc gtgcgccgga gagtgaacat tgttttttta tttacccgat
      301 ggaaaaaata gcggtgaagc aactgcgaaa aaacgaattc atcggagttt gtcaccgcca
      361 gatcaataag ctgatacaac ttcctttccg tgagcaatcg cacaaaatgg tagtttggca
      421 accggtaagc ataaaggccg ataaacacca ctatcacctg catcgcttat tacggagtat
      481 cgacaaaaac atactcatca gcaaactgaa aaaagaagag catgctgcgg aggacgaaca
      541 tttcatcagt gaaaaagacc tgcgcgaaaa attcattcta tttccggaaa taaatagcaa
      601 cacacaaaag atcattgaac aatgcagcat acagctcgaa tacaaaaccg taaaaaacag
      661 aaaacacttc acctcaacag aaaaagatga ccgcgaactc ctccacaaac tggcgaaaga
      721 agggcttgaa taccgctacg gaaaaaaaaa taccagagcc cgtgaacgtg tcgaaaaaga
      781 actgcgcatc atagaggaac ttgatttttc agcttatttt ctcattacac acgacatcat
      841 ccactacgca cgcagcagag gattccacca tgtaggaaga ggttccggcg ccaacagcat
      901 tgttgcgtat tgcatgggta taacagatgt ttgtccgatc gaactcgacc tctacttcga
      961 gcgcttcatc aatccccatc gcagttcgcc tcccgatttt gatattgatt tttcatggga
     1021 cgaacgcgac atcatcattg attatatttt taagcgttac ggcaaacagt acacagcgca
     1081 aattgccacc tatacaacat tcaagggaag ctcaatcatt cgcgaactgg gaaaagtgtt
     1141 cggtctgccc aaggaggaga ttgacggact ggccaatcat cccgataaaa tcgagcagcg
     1201 cgacaacatc accaaagcga tttttcatta tggcaagatg atggaaaact tcccaaacta
     1261 ccttggcatc cacgcaggtg gaattattat ttcggaagaa cccctgtata attacaccgc
     1321 attgcaaatg atgcctaaag gatttccgat tgtgcattgg gacatgtatg tagcagaaga
     1381 tataggattt tacaaatatg atattctatc ccagcgcggt ttggggcata ttaaagacgc
     1441 tgtggatatt gtacaaaaaa acaggaaaat caatatcgac atccacgatg taaaaaactt
     1501 taagaccgac agtaaggttc gtaatcagat tcgttctgcg aacaccatcg gttgctttta
     1561 cattgaatcg cctgccatgc gccaattgct tacaaaatta cgatgtgaca attacctcac
     1621 acttgtggcg gcctcgtcga ttatacgtcc gggcgttgcc agcagcggaa tgatgaaaga
     1681 atacattcag cgttttcatc acccgaaggg atttcagtat ctgcatccta aaatggaaga
     1741 attattacag gaaacgtatg gcgtgatggt gtaccaggaa gacgtgatca aggtagcaca
     1801 tcactttgcc ggaatagatc ttgccgatgc agacatctta cgaagagcga tgagcggaaa
     1861 atacagaggc aaaggagaga tggaacgcat taccgaaact tttttccgca actgcagaga
     1921 acgtggttat ccggaagcca tagcaaagga agtatggcgg caaattgaaa gtttttccgg
     1981 gtactccttt tcaaaagcac attccgcatc ctttgcagta gaaagctatc agagtcttta
     2041 cctgaaaacc tatttcccac tggagttcat ggtgggtgtc atcaacaatt ttggcggatt
     2101 ctaccgcacc gaactttatg tacacgaagc gcgaatggca ggcgcaagta ttgaagcacc
     2161 gtgcgtgaat aaaagtcatt atgttacaag catcaccggc accactattt ggctgggatt
     2221 tgtgcacatg caacacctgg cgcgcaacac ggtaaacatg ctactcgaaa accgtcatga
     2281 aagcggcgcc ttcgaatcgc tcgaagatct tgtagcgcga ctacccatta cgctggatca
     2341 acttgtattg cttatacgta tgggatcact gcgttttacc ggaaaaagca aacaggaatt
     2401 actatggcag gcgcattttc tcttcaacgg aaaaaagcgc agcgcaccct caaatgattt
     2461 gttcaaggca gaagtgagaa aattcgattt gcctgcattc gacgaacacc cactgcaaaa
     2521 agcatacgat gaaatcgaac tcctcggatt tcccctgtgc ggttggtttg atctgctgaa
     2581 aacaaaattc agaggcgaca tcatggccca tgaaatgctg caacactgcg gagaaagcat
     2641 ccgcatgctg ggttatctgg tcaccaccaa acccgtacgc accaaacacg gagatctcat
     2701 gtatttcggt tgtttcatcg attataaagg gcaatttttc gacaccgtac atttccccga
     2761 cacgctcgaa cgttaccgct ttaaaggaaa tgggatttac ctgatgcggg gaacagtagc
     2821 cgatgaattc ggatctgcga gtctgcaggt agagaaaatg gaaaaaatgg aatatgtccc
     2881 gatacagaac tgaacaaaaa ttaattcagg gacgacaata caccatcaca aaatcgttca
     2941 tcacataccc atcaccaata tcgaaatcgg caacctgatc gatacgaaaa cccagtttaa
     3001 aataaaaatt gatggaacgg taattctgtc gattaacggt taaacgtact gttgtgattt
     3061 gcggataagc atcgagaata aattgaaaca cccttgcacc aacacctttt ccctgcatgg
     3121 aatcatcgat atagaattta tgcaaaaaca aatgatgtac ttctttaaga ctaacagcga
     3181 caaaaccaac aggaagttca tcttcaagga tcagataaaa aacctgtccc tcctgaaatt
     3241 gattggcaag tgcatcggag gaatacattt tgttcagcat gtattccacc tgttcagtac
     3301 cgataatggg aggataatgc acacgccaga ttttctgagc cagggcggcg attacagaaa
     3361 tatcattcgg tgaacatttt ctccaattaa tcatactgca aatctataat aagaatggga
     3421 taagtagtaa attagatgaa aattcggaat tgggattttt taaaaagcaa ttaatacatc
     3481 gccaacgtgt gttattttaa gagtaatagt cagaaagttg aagacattga atcgcgctac
     3541 atggcaaaat tcaatgatcc gacccaacac cgaccgaaat accgggaaaa cggatttcaa
     3601 ttcccgaaat gcgctgtgat caccatgaaa gatccggcaa gtattgatca tattcgctgg
     3661 ggactcattc cgtcgtggac ccgcagcagg caggatgcat ttgagataag gatcaaaaca
     3721 ctcaatgcaa gggctgaaag tatttttgaa aaaccatcct tcaaaaccgc catactcagc
     3781 aaacgctgtt tggttccggc aacaggatat tttgaatgga tgcatgaagg gaaaaaaaag
     3841 acgccctatt ttatttcgtt gaatgatgca ccattgttct cgatgggtgg aatacacgaa
     3901 agctggatag acccggaaag cggcaaagca atagaaacat tctccattgt tacgactgag
     3961 gcaaatttac tgacgggcaa gatccacaac agcaaacagc gtatgccact tatcctcgaa
     4021 agagcggatg aagaaaaatg gctcgataat aagttatcgc atagtgagct tgaaaaactg
     4081 atgcgccctc acgcagatca aaacatgcaa gcctggacca tctccgataa actattgcaa
     4141 aatgcagaag acggcgatcc gaagatcatt gaagaatggg ttgatccggc cggattgcag
     4201 ggaagtttgt tttgattaaa tgtgatcaga ttcgcaatcc cattatacag atatcatcga
     4261 tttgctccaa ttcaccgcgc cattcgacca agcggttcca aagcaatttc tcctgttgat
     4321 tcatagggat tgtgcagata tccagcaaca actttttcag gtttccttcc ttgaattttt
     4381 taccccccgt ttttccggaa gataattccc caccaaactg atcaccatag ccatcggaaa
     4441 aaagatacag acaatcatca gcctgcaaca ctattcgctg cgctgtaaaa ttgcgtcgtt
     4501 cgaggtattt tccaacaggt tgtttatccg gtttaagttc tgtgatttca ttctttcgaa
     4561 taatcaccaa cccattattg gcacccgccc agaacaactc tttggtttcg gagttataac
     4621 taccgagaga gatatccatt ccatcgcgaa cttcttcacc accttttgaa aacgtttcca
     4681 gaaccagatc ggtaacctta tccagaatct taccgggatt ggttaatcca aattcattta
     4741 cagaacgact taaagcattg gaacaaacca cgctcaccat tgcacccgga acaccatgtc
     4801 ctgtacaatc tgcaacagca aaaattacga taggtcctac agtatccaga aagaaaaaat
     4861 cacccgcaac aatatccttt ggctgataca acacaaatga atcgcgaaaa tgatcgctgt
     4921 aagctgaaac aggaggaaga atggcagatt gcaatcgctt ggcataagta atggaatcca
     4981 tcacctcttt gttcttctct tcaaccaggt gtttctgacg cgttacttcc agtcgctgtt
     5041 tttcaatgat ctcattctga cgtttggtaa tccgaaaccg ctgaaacaga aaaaatgaaa
     5101 acaacccgac aaataacaca cccgtcgaca aaaacacaat aatcaggttt cttcgcttac
     5161 gttcttcgcg acttattgca tttagtttct ccttctcaag tgcttcttcc tttcgtactc
     5221 tgtcttcgtt tttctccact tcgtattgcg tgcgtaaatc actgatctcg ctcagtcggg
     5281 aagacttgta tatagaatct ttaatctcaa ggtatttttt tccaatatga acagcatcct
     5341 tatactttcc ttgtttttca agcacctgaa aaagaattcg ataactggat tctctttgac
     5401 tgcgtgaaaa attggggtta ttaattgaca aggccttgtt caataattct tcggctttcc
     5461 tgaattctcc tttttgctcg tataaaatcg caagattatt caactcttca acagtaggct
     5521 cctgaccgag ttccttacgc aatgcaagac tgaaattgta ataatattct gcactatcca
     5581 aattgttttg cttacgatga attgcaccca tgttgtttga aaaactggct aattcgacct
     5641 tgtcgccgat cattttgtaa aacgacaata caccggtgta gtaataataa gcgctatcga
     5701 aattatcggt ataaaaataa acagcagcta aatttccttc tgcatatttc tgataaacca
     5761 gatcaccact tttctcagaa gattcttttg tcttgataaa ataaggacgc gcatgctcat
     5821 aatcccttag tttaaaatag atcatcccaa tattgagcga aagcacattg atgtattgcg
     5881 gtttgtttaa acgttctgcg actttaagtc ctgccaacat ataatacaag gcattggaat
     5941 aatcttcatt gatcaaaaag tgtactccca gattggtgaa aatgccaatc acattatccc
     6001 aacgctctac tctggcaaaa taaaaaaacg cttttgagaa atactttttg caggaatcag
     6061 cctgattcag ataatcatac gacaatccca gctctcccca aatatatcct gtagcatggt
     6121 aatctttcac cagggaacac tcttccaatc caacatatcc taaatcaatg gcggtctgat
     6181 aatcaccgga tctcctgatt gctttcatct ggtagaaata cgccagggtg cggctggtat
     6241 caggcttcac tgaggcagtt gcctttcgca tggaatcgat atcgaaaccg gcattggcat
     6301 ttacggacaa aacacacagc gaaaaacaaa gcagaagaaa acgcaacatt gttcaggatt
     6361 tccctcaata tactaaaagt ggtcaatcac ccccaaacca aaaagtgcga aatcgtattt
     6421 tacagggtct aaagcatcaa attccctgag cgattgcgtc aactcctctg ctgcaagcca
     6481 atcgttctgt gtccgttgca aaagccccag ttttcgtcca acattaccgg tatgcacatc
     6541 caaaggacaa atcaaatcgg ctgccgaagt gtttttccag attccgaaat caaccttccg
     6601 tttgttgctc cttaccatcc agcgtaaaaa catattcaac cgtttagcag cagatccact
     6661 cacaggatca ctgatgtgct tcagtgttct tcgttcctgt aaatgcattt caaaaatcct
     6721 atgcaaaagc gcgatccgtt caaacgctgt tccttctccc gataaaacac cttcaagtcc
     6781 gccatcttca ctgtagattt tgcgaagcgc aagaagtaca ccgcgaaaat cggaaccatt
     6841 aaacgtgcga tgcacaaaag cagaacagcg ttccaattgc gaaggattgg catccataac
     6901 aaaatcgtaa ggagaattcc ccatgatctg catcattctg cctgcatttt ttacaatggt
     6961 aatacgctgc ccccaagcga tagaagccga gagaaaaccg gagatttcga tatcctcctt
     7021 aaggttgaat tggtgtggaa tagaaatggg atcagcttca ataaaatcag ttcgttcaaa
     7081 ccgatccgtc atctcatcca taaattgttt gatatcgccg gaaaacattt tacggaacaa
     7141 taactccgtc gcgaatggta agcttgcgat cggccatgtt agcgagttct tcgttatggg
     7201 tgacgataac aaaagtctgg ttgaacttat cgcgtaactc aaaaaataat ttgtgcaatt
     7261 cccgggcatt aaccgaatca agatttcccg aaggttcatc ggcaagtacc aaagaaggtt
     7321 tattcatcag cgcgcgggca acggccaccc tttgttgttc tccgcccgac aattcagatg
     7381 gcttgtgatg catccggtct ttcaatccca gataatcgag caattccact gcacgaggtt
     7441 ctgcatcgcg caacgaattt cctgcaatga gtgcaggcat acacacattt tccagcgcat
     7501 tgaattcggg taacaactga tgaaactgaa aaacaaatcc aatgttcttg tttctgaacg
     7561 aagcgagacc cgcgttattc attcccgcaa tatcctgctc gttaaagagg tatgtcccct
     7621 tatccggaac gtccagcgta cccagaatat gcaagagtgt cgacttcccc gctccggaag
     7681 aacctacaat tgaaatgatt tcaccggcgg caatatcaag atcaatcccc ttcaggacct
     7741 ttagagcacc gtaactcttc tcgatggagc gaatactgat catacagtca aataaacgaa
     7801 caaaatcgaa cttttccata cactgtaagc aagtctttgt ggaaatctcc tgccagccca
     7861 caccctcaaa agcagatcac agtgtatttt agcagtatga atccttcact ggtcattacc
     7921 attctcctca gctattttgc gctgctggtt accatctcct ggttcacttc ccgcaaagct
     7981 gatgccgatt cctacttcct gggaaacaaa aaatcgccct ggctggctgt ggcattcggt
     8041 atgctgggcg attcattgtc gggtgttaca ttcatctctg ttcctggtgc tgtttatacc
     8101 ggtaatttcg cttacctgca actggtattc ggctattttg caggatactt tgtgatctcg
     8161 gaaattttat tgccattgta ttaccgattg aatgtgacct ccatctattc ctatctggga
     8221 atacgcatcg gatcgtattc ggaacgcaca ggatctgttt attttctttt gtcgcgctta
     8281 cttggtgcgg gcggaagact ttacctcgct gcaggtgtga ttcatttgtt tgtattgcag
     8341 caactcaaca tcccctttgc ggtttctgtt gccgtaatca ttctgctcat gttggtgtac
     8401 acacttaaag gaggaattaa aacactggtc tggaccgatg tccttcaatc ggctgttctg
     8461 ttgttggctg tcaacctttc aattcttgcc attatgcatt caaccggaaa gggaattgga
     8521 gaaatgatca gtggaatttg gtctgatccg cacaccgaaa cctttttctg ggattggaaa
     8581 ttgaaaagtt ttttcccgaa aatgttcatt ggaggaatgc tcaccgcaat tgccatgacc
     8641 ggtcttgatc agaacatgat gcaaaaaaac ctcagttgca aatcg
//
LOCUS       DAOMYG010000072         8683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2699_length_8683_cov_8.228906, whole genome shotgun sequence.
ACCESSION   DAOMYG010000072 DAOMYG010000000
VERSION     DAOMYG010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8683
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2699_length_8683_cov_8.228906"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..1411
                     /locus_tag="PK610_03630"
     CDS             35..1411
                     /locus_tag="PK610_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ35047.1"
                     /translation="MIFRKRNRADEETRYSFGRDTWKQFRRNRPAYISFYVLLFMLFI
                     GMFASFLANDQPLYASYRGKTFYPAFAEHWIGKSVFGAEGVDSVQHPVTGNWERIQYD
                     IADWRALELGAVIWPLIPFSSSTQDILNRDFVSPDDEHFRRNEKGEVITATGKFRHRM
                     GTDVLGRDVLAGIIHGTKISLLVGIVSMSIALLIGLLLGSLAGFFGDHRIRLSRIRLF
                     LLVPGLLLAWFYAFVLRGYTISDAFGESTGSGFVQLLLSILLFVLILMMIIYGGKFLE
                     FGFFAKQVFLPVDSLISRGIEIFNSMPKLLLIITISAVIEERSIWLLMLIIGLSSWTE
                     IARFVRAEMLKVRELEYVQAARSMGLSEWRIIVKHALPNAMAPVFIYVAFGIASAILV
                     ESGLSFLNIGVPDDVVTWGSMLNVGRTEPEAWWMIVYPGLAIFITVTVYNLIGEGLRD
                     ALDPRLRK"
     gene            complement(1412..2992)
                     /locus_tag="PK610_03635"
     CDS             complement(1412..2992)
                     /locus_tag="PK610_03635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRJ35048.1"
                     /translation="MQNLINFLSKEKNLVLLFSLLLIPAMFINLGVQPLTADEPTRAV
                     VALEMEYSGNYIVSTINEVFYYKKPPLFNWLICAAFQISGSPSEFVLRLTAVLPMLLF
                     GWYLYKVFKRYTNPKIAFLSVAMFLTNARVISYDSYLGHIDMLYSWITFITFIVIYEG
                     LKKEKYLMLFVVSYFLSALAFLLKGLPTFIFQGTTLIVCFWMMGRFKKLFSLWHLIGI
                     LAFVIPVAGFFYTYNQYNELWGWVDQLWDQSKQRTVLDKAWYENFIHLFKFPVDHVGH
                     LAPWSLLAVPLFYKSIRNKIAENEFVKMMGIIMVANLPAYWLSPGYYPRYLLMLYPII
                     FLILAFAWENKNELLKSYEKDLFKVFAGLGVLISFGPLVLLLIDLGIPINPIFSVLIT
                     ISLLFLSYLMFRKSQMTLYFLALFILVLRIEFNLVAFPHRASTGGVAEYKEFAEQIVE
                     KSKGKKLYTISGVNHAVSFYIERERGEILKRVDFPDTTSYYIIFSDEIFGDYQTIIDY
                     HLSIENRPMKLVKYKQPF"
     gene            complement(2964..3857)
                     /locus_tag="PK610_03640"
     CDS             complement(2964..3857)
                     /locus_tag="PK610_03640"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRJ35049.1"
                     /translation="MFSKKSIKTWIKLLLTTLAIWFVFRKIDGEAILVELKKVNLPLF
                     LLALIAFLASKAVAAFRQNEFYEAAGGRQATVFNLKLTAIGMFYNLFLPGSIGGDAYK
                     VYLIKNNKPSLSTRMLISAAFLDRLSGVVILFFMMVIFVLFSEFTYLHEQWLYWLLIA
                     ALVLCIPVYFIILKFFFKSFSHKFLKTTHLSIWVQIGQVVCSLLLLYSMGINDHQMDY
                     LALFMASSVVAVLPFTIGGIGARELVFIYGAILFPIQEEPAVAFSILFFIITALSSFS
                     GLIFIPSIDKKTNAESDQLPQ"
     gene            4044..5222
                     /locus_tag="PK610_03645"
     CDS             4044..5222
                     /locus_tag="PK610_03645"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014938126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRJ35050.1"
                     /translation="MKEVFIVAAVRTPMGSFNGALSSVPATQLGATAIKGALDKIKLN
                     PNEVQEVYMGSVLQAGLGQAPARQAAKFAGLPDSVVCTTVNKVCASGMKSIALAAQSI
                     MLGDNDVVVAGGMENMSQVPHYLMGSRNGFRLGDAKLTDGMVSDGLTDVYNRQHMGNC
                     AELCAKEKNISREEQDAFAITSYQRSAAAWEAGKFNDEIVPVAVPQRKGDPVIVAKDE
                     EYTNVKLDKIPSLSPVFQKDGSVTAANASTLNDGASAVVLMSKEKMEKLGLKPLAKIV
                     SFADAEQAPEWFTTAPSIALPKALAKANLKVSDISYWELNQAFSVVGLANMKELGLDP
                     SKVDVNGGAVALGHPLGSSGCRIIVTLINVLKQNNGKYGAAGICNGGGGASAMIIENC
                     "
     gene            complement(5280..6617)
                     /locus_tag="PK610_03650"
     CDS             complement(5280..6617)
                     /locus_tag="PK610_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRJ35051.1"
                     /translation="MKTILIIGAGRSSSTMIKYLLEHSKQENWKIRVGDMDLALAEKK
                     IAGHPNGQAFQFNALDPEERKKEIAAADFVISMLPAALHIEVAKDCIALKKDVVTPSY
                     ISSAMKELDTDAKKAGIIILNEIGVDPGIDHLSGMKIIDEIHSKGGTVTAFESYCGGL
                     VAPESDNNPWGYKFTWNPRNVVLAGQGGAAQFIREGSLKYIPYHQLFKRIETIAISGH
                     GEFEGYANRDSLSYRSVYGLENIPTMLRGTLRKKGYCGAWNAFVQLGCTDDSYTMQGS
                     SKMTWREYINSFLDFHPTWTVEEKFCHYLNIGENSDLYKKIKWLGIFEKESIGIEKDA
                     SPAQILQKKLEEKWVLSPSDKDMIVMVHRFIYTLNGKKHELHSSMVYKGTDDTYTAMS
                     DTVGLPVAICTRMILNGLIKDKGVLLPIAKSIYEPVLKELETLGIKFEEKQIS"
     gene            complement(6658..7047)
                     /locus_tag="PK610_03655"
     CDS             complement(6658..7047)
                     /locus_tag="PK610_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HRJ35052.1"
                     /translation="MKNKLIFSGSLLMALAVILGALGAHALKAKLSTDQLLSFETGVR
                     YHLIHALGILALAGFSEQFGEKRLRTIAILMVLGILFFSGSIYLLATRDLYGMSGELR
                     FLGPVTPIGGMLFIAGWILTALSVKRN"
     gene            7125..8609
                     /locus_tag="PK610_03660"
     CDS             7125..8609
                     /locus_tag="PK610_03660"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HRJ35053.1"
                     /translation="MKSNYDLYCDHLRKLADVMFSSAVLQWDQETYMPSKGAEMRAGQ
                     LSTLAGIAHELSVDSTFGELLNKLKDDATLTERQKRNVELSLKDFNDRNKYTTEFVQT
                     LSLAVSEAFVAWDEAKKKSDFAIFSPKLKLIVELKRKEAELLGYKQHPYDALIDQYEP
                     GTTTADVERLFADIRNKLKPLIEKVLKQDAPRDEFMYRFFPRQKQWDLGMDILKQMGY
                     DLEAGRQDESSHPFTIHFNANDVRVTTRVSETNFHEMIWSTIHEGGHALYEQGLPRTE
                     YGLPLGEACSLAIHESQSRLWENNVGRSLSYWKANWKKVLDYFPEQMKDVSVEEFFKA
                     MNKVEPSLIRTNADELTYHFHVLIRFEIEKGLIEGSIEVEDLPQVWNAKYKAYLNIDV
                     PNDAEGVLQDIHWSHGSIGYFPTYSLGSFYAAQLFATACKQDDSIEQSIAAGNMKPLL
                     TWLRKNVHSEGKFYPAPKLIEKVCGEKPDFRYFMEYANAKYGVF"
BASE COUNT         2575 a         1648 c         1879 g         2581 t
ORIGIN      
        1 catttgctga cccaagaata acttataaca ggaaatgatt ttccgcaagc gaaatagagc
       61 agatgaagag acacgatatt ccttcggaag ggatacatgg aaacagtttc gacgaaacag
      121 acctgcctac atttcttttt acgttttact tttcatgctc tttattggaa tgtttgcttc
      181 ctttctggct aacgatcaac ctttgtatgc cagctaccgg ggcaaaacct tttatcctgc
      241 atttgcagaa cattggatcg gcaaatctgt gttcggagcg gagggagtag attctgttca
      301 acatccggtt acaggaaact gggagcgtat tcaatacgat attgccgact ggcgtgcgtt
      361 ggaattggga gctgttatct ggccgttgat tccgttttca tcttcaactc aagatattct
      421 caatcgcgat tttgtttcac cagacgatga acattttcgc agaaatgaaa aaggagaagt
      481 gatcacggcc acagggaaat tcagacatag aatgggtaca gatgttctgg ggcgcgatgt
      541 gcttgcagga atcatacatg gaaccaaaat ctctttattg gttggtatag tctctatgtc
      601 cattgcattg cttattggac ttttgcttgg atcactcgca ggatttttcg gtgatcatcg
      661 tatccgactt agcagaatca gattgtttct gcttgttccg ggtttgttac ttgcttggtt
      721 ttatgctttc gtacttcgag ggtatacgat atctgacgca tttggcgaaa gtaccggttc
      781 aggatttgtt cagttgttat taagtatatt gttgtttgta ttaattctga tgatgataat
      841 atacggtggg aaatttcttg agtttggatt ttttgcaaaa caggtttttc tgccagtaga
      901 tagcctcatc tcccgaggta ttgaaatttt taattcgatg ccaaaattat tattgattat
      961 tacaatttca gctgttattg aagagcgaag tatttggttg ttgatgctga ttatcggact
     1021 aagttcctgg acagaaatag ctcgatttgt cagggcggaa atgcttaagg ttcgcgaact
     1081 tgaatatgtg caggctgcgc gttcaatggg tttatcggag tggagaataa ttgtaaaaca
     1141 cgccttgccc aatgcaatgg cccctgtgtt tatttatgtt gcatttggaa ttgcatcggc
     1201 aatattggtg gaaagcggac tttccttttt gaatataggt gttcccgatg atgttgttac
     1261 ctggggctca atgctgaatg ttggtcgtac agaacccgaa gcctggtgga tgattgttta
     1321 tccgggattg gcaattttta ttacggtaac tgtttacaat ctcattggcg agggtttacg
     1381 tgatgccctc gatccgcgat tgcgcaaata attaaaacgg ttgcttgtat ttaaccagtt
     1441 tcatcgggcg attttcaatg ctgaggtggt agtcaatgat ggtttgataa tctccgaata
     1501 tttcgtctga gaaaatgatg taataggagg tagtgtccgg aaaatcgaca cgttttaaaa
     1561 tttctccccg ttcacgttca atgtagaatg aaacggcatg attaacacct gaaatggtgt
     1621 acagtttttt ccctttgctt ttttctacta tttgttcagc aaattccttg tattcggcta
     1681 cacctccggt cgatgcacgg tgtggaaagg caaccagatt aaattcaatg cgcaaaacaa
     1741 gaataaaaag ggcaagaaaa tataaagtca tttgactctt cctgaacatc aaatacgata
     1801 aaaaaagcaa ggagatagtg attaatacag agaatatggg attgatagga atacccagat
     1861 caatgagcaa taatacaagg ggaccaaatg aaatcaaaac acctaatcct gcgaaaacct
     1921 tgaataaatc cttttcgtag gattttaata actcgttttt gttttcccac gcaaatgcca
     1981 gaatcaggaa aattataggg tacaacatta ataaatacct ggggtaatat ccgggtgaaa
     2041 gccaatatgc aggcaaattg gcaaccatga tgattcccat catttttaca aattcgtttt
     2101 ctgcaatttt atttcggatc gatttgtaaa ataaaggcac tgccaataat gaccaaggag
     2161 ccagatgtcc gacatgatca acgggaaatt tgaatagatg aatgaaattc tcataccatg
     2221 ctttgtccag tactgtacgt tgtttggatt gatcccaaag ttgatctacc caaccccata
     2281 attcattgta ctgattgtaa gtgtagaaaa atcctgcaac gggaataaca aacgccagga
     2341 ttccgatcag atgccacagg gaaaataatt tcttgaaacg ccccatcatc cagaaacaaa
     2401 caatcaaagt ggttccctgg aaaataaagg tgggtaatcc ttttagtaaa aatgccagtg
     2461 ccgagaggaa ataggataca acaaacagca tcaggtattt ttcttttttt agtccttcat
     2521 aaattacaat aaacgtaatg aaggttatcc aggaatataa catatcgatg tgacccagat
     2581 aggaatcata tgaaattact cttgcgttgg tcaagaacat tgcaacactt aaaaaagcga
     2641 tttttggatt ggtgtatcgt ttaaaaacct tgtataaata ccaaccgaag agcagcattg
     2701 gcagaactgc ggtcaggcga agtacaaatt cgctaggact accgcttatt tgaaatgcag
     2761 cacagatcag ccagttaaaa agtggtggtt ttttgtaata gaaaacctca ttgatcgtag
     2821 aaacaatata attacctgaa tattccattt ccagtgcaac aactgcacgc gttggttcat
     2881 ctgcagtgag aggctgaact cccaggttga tgaacattgc cggaatcagc agcaatgaaa
     2941 aaagaagcac aagatttttt tctttactga ggaagttgat cagattctgc attcgttttt
     3001 ttatcgattg aaggaatgaa gattagtccg ctaaaagagg ataatgcagt tatgatgaaa
     3061 aataaaattg aaaatgccac tgcaggttct tcctgaattg gaaataaaat cgcaccgtaa
     3121 ataaaaacaa gctctctggc tccgattcct ccaatagtaa acggcaaaac agcaacaacc
     3181 gaggaggcca taaaaagtgc gaggtaatcc atttgatggt cgttgattcc catggaatag
     3241 agtaggagca gagaacatac tacctgacca atctgtaccc agatagaaag gtgggttgtt
     3301 tttagaaatt tgtgactgaa cgatttgaaa aagaatttca gaataataaa atatacagga
     3361 atgcacagaa ccaatgcggc aataagcagc caatacaacc actgttcatg caggtaggtg
     3421 aattcgctga acaatacaaa gatcaccatc atgaaaaata aaatcactac tccacttaag
     3481 cggtcaagaa aggcagcaga aatgagcatt cgtgttgata gtgatggttt attgtttttt
     3541 atgagatata ctttataggc atctcctcca atacttcctg gtaagaaaag attgtagaac
     3601 atcccaattg cagtcagttt aagattaaaa actgttgctt gtcttccacc tgctgcctca
     3661 taaaattcat tttgacggaa tgctgctacg gccttggagg ctaaaaaggc aattaaagcc
     3721 aacagaaaca atggaaggtt cactttcttt agttcaacta ggatagcctc tccgtcgatt
     3781 ttgcggaaga caaaccatat tgccagggta gtcagcaata gttttatcca ggttttgata
     3841 ctttttttcg agaacatcgg cgccaaagat cgcagttcct gcaaaagtaa ctcactgttg
     3901 cgacgtaaaa ttgatcattt caggtcaatt tgacgcaaat agtgctgtat ggggttcatt
     3961 ttctgcgata ttcccttttt ccaccaagag aaaccacgag gtcatttttt tgtaatttca
     4021 ccctctgaaa atcaactatg atcatgaaag aagtttttat cgttgctgcc gttcgtactc
     4081 ccatgggaag ctttaacggc gctttgtcaa gtgttcctgc tactcaactt ggtgctactg
     4141 ccattaaggg tgctttggat aaaatcaaac tgaatccaaa cgaggttcag gaagtttata
     4201 tgggaagcgt tctccaggcc ggtcttggtc aggctcccgc ccgtcaggct gctaagtttg
     4261 caggattgcc cgatagtgtt gtttgtacta ctgtaaataa agtttgtgct tccggaatga
     4321 agtcgattgc cttggctgct caaagcatca tgcttggtga taatgacgtt gtagttgccg
     4381 gaggaatgga aaatatgagt caggttcctc attaccttat gggctcccgt aacggattcc
     4441 gtcttggtga tgctaaactg accgatggaa tggtttctga tggtctaacg gatgtttaca
     4501 atcgtcagca catgggtaat tgcgctgaat tgtgcgcaaa ggaaaagaat atcagccgtg
     4561 aggaacagga tgcttttgcc atcacttctt accagcgttc tgctgctgca tgggaagccg
     4621 gaaaattcaa tgatgaaatt gtaccggttg cagttcctca acgaaaaggt gatcctgtta
     4681 tcgttgcaaa agatgaagaa tacacaaatg taaaattgga taaaattcca tcgctctcac
     4741 cggtatttca aaaagatggg tcagtgactg ctgctaatgc ttctactttg aacgatggtg
     4801 cttctgcagt agtattgatg agcaaggaaa aaatggaaaa gctgggtctg aaaccgcttg
     4861 ctaaaattgt ttctttcgca gatgctgagc aggctcctga atggtttaca acagctcctt
     4921 ccatcgctct tcccaaagcg cttgcaaaag caaatctaaa agtttcagat atcagttatt
     4981 gggaacttaa tcaggcgttt tctgttgttg gacttgccaa tatgaaagaa cttggtttgg
     5041 atccttcaaa agttgatgtg aatggtggtg ctgttgcgct tggacatcct ctgggaagtt
     5101 caggttgtcg tattattgtt acactgatca atgtcctgaa acaaaacaat ggaaaatacg
     5161 gtgctgctgg aatctgtaac ggtggtggtg gagccagcgc aatgattatt gagaactgtt
     5221 aaatctattt ccaacaataa ttgaaaagag cgtttcaatt tgaaacgctc tttttatttt
     5281 caggagattt gcttctcttc aaactttatt cccagtgttt cgagttcttt taacaccggt
     5341 tcgtaaatgc tttttgcaat gggaagaagg actcccttat cttttatcag accattaagt
     5401 atcattcttg tgcaaatggc aaccggtaat cctacagtat cactcattgc tgtataggta
     5461 tcgtctgtac ccttataaac cattgaagaa tgcaattcat gcttctttcc gttcaaggta
     5521 taaataaatc ggtgtaccat tacaatcata tccttatcgc tgggagataa tacccatttc
     5581 tcttcgagtt ttttctgaag gatctgcgcc ggactggcgt ctttttcaat tccgatggat
     5641 tccttttcaa aaatacccag ccatttgatc tttttgtaca gatcactgtt ttctccaata
     5701 ttgaggtagt gacaaaattt ttcttcaacg gtccatgttg gatgaaaatc gagaaatgaa
     5761 ttgatgtatt cccgccaggt cattttactg ctgccttgca tggtatagct gtcgtctgta
     5821 caaccaagtt gaacaaaggc attccacgct ccgcaatatc cttttttacg taatgttccc
     5881 cgcaacatgg ttggaatgtt ttccaatccg taaacagacc tgtagcttaa tgaatcgcgg
     5941 ttggcataac cttcaaattc gccatgacca cttatagcga tcgtttcgat gcgtttaaat
     6001 aattgatgat aggggatgta ttttaacgat ccttcacgaa taaactgtgc tgcaccaccc
     6061 tgaccggcaa gtacaacatt acggggattc catgtgaatt tgtatcccca ggggttattg
     6121 tcgctttcgg gagcaacaag tcctccgcag taggattcaa aggcagtaac ggttccacct
     6181 ttgctgtgga tttcatcaat gattttcatt cccgacagat ggtcgattcc ggggtcaact
     6241 ccaatttcat tgaggataat gatgcctgct ttttttgcat cggtatcgag ttccttcatt
     6301 gcagaggaaa tataggatgg agtaacaaca tcctttttca atgcaataca atctttcgcc
     6361 acttcaatgt gcaaggctgc aggaagcatg gagatgacaa aatctgctgc tgcaatttcc
     6421 tttttgcgtt cttccggatc aagtgcgttg aattgaaatg cttgtccatt cgggtgaccc
     6481 gcaatctttt tttctgcgag tgctaggtcc atatcgccaa cccgaatttt ccagttttcc
     6541 tgtttgctat gctccaaaag gtatttgatc atggtcgaag aagaacgacc tgcaccaatg
     6601 atgaggatgg ttttcataag ttgaatttca gggtaaaggt aaacacttcc gtaatattca
     6661 gttgcgtttt acggaaagcg cagttaagat ccatccagca atgaacaaca ttcccccaat
     6721 tggagtaacg ggtcccagaa aacggagttc accgctcatg ccatagagat ctctggttgc
     6781 cagcaggtaa atggaacccg aaaaaaataa aattcccaac accattaaga tggcaattgt
     6841 tcggagacgt ttttcaccga attgttcaga aaagcctgca agtgctaaaa ttcccaatgc
     6901 atgaatcagg tgatacctga ctccggtttc aaaggaaagg agttgatctg tacttaattt
     6961 agcctttaac gcatgcgctc ctaatgcccc gagaataaca gccaaagcca ttaacagtga
     7021 accggagaaa ataagtttat tcttcatgat tcaaatgtaa atgaataaag cgctttcttt
     7081 taggttcgta gtaaatagag cgaaaaaaag gtattttcgg gataatgaaa tcgaactacg
     7141 acctctattg tgaccatctg cgaaagcttg cggatgtgat gttttcctca gcggttcttc
     7201 aatgggacca ggaaacgtat atgccttcaa aaggagcgga aatgcgtgcc ggacagctat
     7261 ctaccctggc tggaatcgct catgagttaa gcgttgattc tacatttgga gagctattga
     7321 acaaattaaa ggacgatgca actttaactg aacgacagaa acgaaatgtt gaattatcac
     7381 tgaaggattt taacgataga aataaataca ccactgaatt tgttcaaacc ctttcactgg
     7441 cagtttctga agcatttgtg gcttgggacg aagcaaaaaa gaaaagtgat ttcgccattt
     7501 tttctcctaa attaaaactc atagtagaat taaagcgaaa agaagcagaa ttgttgggct
     7561 ataaacaaca tccctacgat gcacttattg atcaatacga accgggcaca actaccgctg
     7621 atgttgagcg tctgtttgca gatattcgga acaaattaaa accgcttatc gaaaaggtat
     7681 taaaacagga cgctcctcgc gacgaattca tgtatcgatt tttcccacgg caaaagcaat
     7741 gggatttggg aatggatata ttgaaacaaa tgggctacga tctggaagcc ggtcgacagg
     7801 atgaaagttc acatcctttt accattcatt tcaatgccaa tgatgttcgt gttaccacac
     7861 gcgtcagtga aacaaatttt catgaaatga tctggagtac cattcatgaa ggaggtcacg
     7921 cactttacga acaaggtttg ccacgaacag aatacggtct tcctttgggc gaagcatgtt
     7981 ccttggcaat ccatgaatcg caatcacgct tgtgggagaa taatgttggg agaagtttga
     8041 gttattggaa agcgaattgg aagaaagttt tggattattt tccggagcag atgaaagacg
     8101 tttctgttga ggagtttttc aaggcaatga acaaggttga gccatctcta attcgtacca
     8161 atgccgatga gctgacctat cattttcatg tcttaattcg atttgagata gagaagggtt
     8221 tgatcgaagg aagtattgag gtggaagatc ttccacaggt ttggaatgct aaatacaagg
     8281 cgtatttaaa tattgatgtc ccgaatgatg ccgaaggggt attgcaggat attcattggt
     8341 cgcacggtag tattggatat ttcccgactt attcgcttgg aagtttttat gcagcccaat
     8401 tgtttgcgac tgcttgcaaa caggacgatt caattgaaca gtcaattgcc gcggggaaca
     8461 tgaaaccttt attgacctgg cttcgaaaaa atgtgcattc agaagggaaa ttttaccccg
     8521 ctccaaagct gattgaaaaa gtgtgtggtg aaaaaccgga ttttagatat ttcatggaat
     8581 atgctaatgc aaaatatgga gtattttaat agtagttcag gattgttttt ttcttttgaa
     8641 tgcaaaacaa taccggtaac gtaatgccca aattctacct taa
//
LOCUS       DAOMYG010000073         8653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2716_length_8653_cov_9.697371, whole genome shotgun sequence.
ACCESSION   DAOMYG010000073 DAOMYG010000000
VERSION     DAOMYG010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8653
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2716_length_8653_cov_9.697371"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..344)
                     /locus_tag="PK610_03665"
     CDS             complement(45..344)
                     /locus_tag="PK610_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35054.1"
                     /translation="MSSTATPVLIPKEQIASLRFPSQEVQLSAEERKALFEKLTQATT
                     LGNMDHHKCRITFSDDEGMKVVETTIWATGEKNIVLKHGMTIPIHRIHDVNIYAG"
     gene            complement(406..1734)
                     /gene="tilS"
                     /locus_tag="PK610_03670"
     CDS             complement(406..1734)
                     /gene="tilS"
                     /locus_tag="PK610_03670"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRJ35055.1"
                     /translation="MHTLEKAFRKVIDEALPHLEEQHFLLAVSGGVDSVVLADLFFRS
                     RLNFTIVHCNFQLRGEESERDESFVKQLASRYDVECLVRSFDTLSYAGTNHLSIQMAA
                     RELRYDWFRELMRTQRYSQLVTAHHRNDQAETFFINLLRGSGIEGLSGMRILEGTLFR
                     PLLPFHRREIEGFANDTRLKWVEDSSNTSSDYLRNRIRMELIPVAEDIRSGAVEQIGE
                     AMDRLADVNAYYKLHMEDERRRILQKNEFGYEFVMEDVMKSPVPRLMLFELMRGFGFR
                     YPVIRQLLDHHQNNSGKKFFSSTHRAVLSRGVVVVTRITPEDLQIYEIVEGADNLQEP
                     IRLTIRRKKYTGEIPTSPFHVVLDASRIEFPLALRRWHKGDSFIPLGMKGRKKLSDFF
                     IDLKLSLVEKEQAWVLCSGDQLLWVVGHRIDERYKAHPETVEVLEISVEI"
     gene            complement(1744..2904)
                     /locus_tag="PK610_03675"
     CDS             complement(1744..2904)
                     /locus_tag="PK610_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HRJ35056.1"
                     /translation="MMESVSSFSEWYAQLSLSEENFLLLNCGPDAQPVFYSGKKWESG
                     PVEKIPEGDIVLALAYELNTAFENIQCGSDKGMDVPSIMWMHPATTIALPPLGDFLVP
                     AIEKQKRINFHGRISKEEYLQSVLSLQEHIRKGDIYEVNFCMEFFAEEVEVDPFVVFA
                     NLHSITRAPFSAFLRREEQFLLCGSPERFVQRKGDRLLSQPIKGTIRRGKSQEEDKVL
                     REQLRNDSKERSENVMIVDLVRNDLSRIAIRSSVRVDELMEVYTFPTVHQMISTISCE
                     LREGVNTYDILKAAFPMGSMTGAPKVKAMELIARYEQSRRGWYSGSVGFRKANGDCDL
                     NVVIRSLFYDRQKRYLSFSVGSAITAASDPEREYDECLLKAEAMIKAVNASS"
     gene            complement(2901..3905)
                     /gene="hemH"
                     /locus_tag="PK610_03680"
     CDS             complement(2901..3905)
                     /gene="hemH"
                     /locus_tag="PK610_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRJ35057.1"
                     /translation="MKTAILLLNLGTPDSTKTSDVRKYLREFLMDGRVIDINPVGRWM
                     LVNLIIAPFRAPKSAKEYRKLWTPQGSPLLLHGLKVKEDLQKILPENYSVEFAMRYQN
                     PSMQSVLEKMSRANYDRIIILPLYPQNASSTTGSSLEKAMQIISKWWVIPEIKMISQY
                     YDHPKYIQAFVERGREHDLKSYDHVVFSFHGVPERHVDKVYFDNRPCKDHHCEEGITD
                     ENKFCYKATCYNTAKRIAAELNISEYTVVFQSRLGRDPWLEPYADKTIEQLAKNGVKR
                     LLVFSPAFVADCLETTVEIGEAYKEMFEEHGGEHLQLVNSLNDHPTWVECLRDLAMQS
                     "
     gene            complement(3948..4376)
                     /locus_tag="PK610_03685"
     CDS             complement(3948..4376)
                     /locus_tag="PK610_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRJ35058.1"
                     /translation="MHKESLRITQQYVNDTAYAIVGCAIEVHRELGPGLLESVYENCL
                     VKELQSKGFEVKRQVTVPVLYKGEDAGAALKLDVLVEDLIIIELKAVESILPVHKAQL
                     LSYMKLAGKPKGLLINFFSENITKSGLVPMVNELFNELPK"
     gene            complement(4401..5804)
                     /gene="lpdA"
                     /locus_tag="PK610_03690"
     CDS             complement(4401..5804)
                     /gene="lpdA"
                     /locus_tag="PK610_03690"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014938447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRJ35059.1"
                     /translation="MSNYDVVIIGSGPGGYVCAIRCAQLGMKTALVERYNSLGGTCLN
                     VGCIPSKALLDSSEHYHNASHTFKEHGIDVKDLKVNLKQMIERKNGVVSQTVDGIRFL
                     MKKNKIDVYNGHGSFQSNTKVDIKSDDGKVTTIEGKKIVIATGSKPSAFPGMEIDKKR
                     VITSTEALNLNEVPKHLIVIGGGVIGLELGSVYARLGAKVSVIEYAPSIISTMDGTMG
                     KELQKSLKKLGFEFYFQHKVLSVKGSAKEATVTAEDAKGQKVEFKGDYCLVSVGRRPY
                     TDNLGLENAGVKLDERGRVHVDDHLQTNVPNIYAIGDVVRGAMLAHKAEEEGVFVAEY
                     MAGQKPHMNYNLIPGVVYTWPEVAAVGQTEEELKKAGRKIKIGQFPFKASGRARASMD
                     LDGLVKVIADEETDEVLGVHMIGPRAADMIMEAVVAMEYRASAEDIARICHAHPTFTE
                     ALKEAALDATGKRALHI"
     gene            complement(5958..7448)
                     /locus_tag="PK610_03695"
     CDS             complement(5958..7448)
                     /locus_tag="PK610_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ35060.1"
                     /translation="MENTIYWNETNIYQANIDLYAGLTVNQHTIDGPVVVKYNSNIIY
                     RAGNQVVLEPGFSTEPGCYFEAVIGPCENNPCPAPPFIPQSLELCGNTTVVLGPTSPP
                     DVGLLFSWSPATYLDNPNSPNPTFTAPSGVGTMNYTLTVTSVCGYLFNPPFSFEQQLN
                     YPVSIYYNDNPDPNPVLSLSNIQQSHMVFSMDAQVGPSTEKVCISLTDVSTGQVIATE
                     CFYADSDFDCCSFSFSNFDFSDATRLLIDPCKDYQVSVQVNNKCDANLATQSFLWNND
                     PTFSLVVLPNVITPNSDGVNDSWRVQATGVKRYEVEVRDINQRVYFATDIVNSFDFKI
                     WDGICNQGWPACNPGSPCLSDGTYFYILNLIDCNNVVHDYTGFITLLDGTPNGCLNPR
                     AADVSGNQQAEMNPEAESFLSTENQEIAVGLHLFPNPSDGRLTIVHDHLMGEIQILDV
                     SGRLVKSTSTSGSQTHLELGYLSNGHYLIKLHDQEGKVYFARWVKQ"
     gene            complement(8174..>8653)
                     /locus_tag="PK610_03700"
     CDS             complement(8174..>8653)
                     /locus_tag="PK610_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35061.1"
                     /translation="NDTKQREIFAEGFGPGYLYSLNFAASFGKKQFNRYRFGISYYYD
                     YLINSSDPGSEIILITEANRSFLKRKKSSFFVGLGFLLLPDIRAIFKLDNYSCSANYN
                     GQCSKLPYFYLYGSLGYKYSFSSGIFTGFSFYVFSDFVNNYYPYGGVSIGKNLNYKK"
BASE COUNT         2454 a         1997 c         1783 g         2419 t
ORIGIN      
        1 cgcaggatga acatctcctt tattcagctg aataaaggag atgttcatcc tgcgtaaata
       61 ttcacatcgt gaatacggtg gatggggatt gtcattccgt gtttcagaac aatgttcttt
      121 tcgccggtag cccaaatggt ggtctctacc accttcatcc cttcatcgtc cgaaaacgtg
      181 atgcggcatt tgtggtgatc catatttcca agggtggtgg cctgtgtaag cttttcaaaa
      241 agtgctttgc gttcttcagc gctcaactga acctcctgag aaggaaaacg aagagatgcg
      301 atttgctctt tgggaatgag tacgggagta gcggttgaac tcatggcttt actggttttg
      361 taaaatgttt ttacgaagaa ttttcgaaat agttatagtc cgggattaga tttctaccga
      421 aatttccaga acctcaaccg tttcaggatg agccttgtac cgctcatcaa tccggtggcc
      481 gaccacccac aaaagctgat cgcccgaaca aagcacccag gcctgttcct tttctaccag
      541 cgatagttta aggtcgataa agaaatcgga aagtttcttt cttcccttca tcccgagagg
      601 aataaaacta tcacccttgt gccagcgacg caatgccagc ggaaattcaa ttcgcgatgc
      661 atcgagtacc acatgaaaag gtgatgttgg aatttcgcca gtgtattttt ttcggcgtat
      721 ggttaaacga atcggctcct gtaaattatc ggctccctcc acaatttcat agatctgcaa
      781 atcttcggga gtaatccgcg ttaccaccac tacccccctg ctcaggacag cacggtgtgt
      841 ggatgaaaaa aacttttttc cgctgttgtt ctgatgatga tcgagcaact gacggatgac
      901 aggataacga aatccaaatc cgcgcatcag ttcaaacaac atcaaacggg gcacaggact
      961 tttcatcaca tcttccatta caaattcata gccgaattcg tttttttgga gaatgcgtcg
     1021 acgttcgtct tccatgtgaa gtttgtaata agcattcaca tcagccagtc gatccatagc
     1081 ttcaccaatc tgctcaacag caccggaacg gatatcttct gcaacaggaa taagttccat
     1141 gcgtatgcgg ttgcgcaaat aatcggaaga agtattggaa ctatcttcca cccatttgag
     1201 ccgggtatcg tttgcaaatc cttcaatttc ccggcgatga aaaggcagca gcggacgaaa
     1261 aagtgtgcct tcaagaatcc gcattccact taatccttca attcctgatc cgcgcagcaa
     1321 attgataaaa aatgtttcag cctgatcgtt gcggtggtgt gctgtaacca actgcgaata
     1381 gcgttgtgta cgcatcaact cacgaaacca gtcgtaacgc aattcgcgcg cagccatctg
     1441 aatggaaagg tgattcgtcc ccgcatagga aagcgtatca aacgagcgca ccaaacactc
     1501 cacatcgtaa cgcgaagcca attgcttcac aaacgattca tcccgctctg attcttctcc
     1561 gcgcaattgg aaattacaat gcacgatggt aaaatttaat cgcgaccgaa agaacaaatc
     1621 agccagtacc acgctatcta caccaccgct tacggcaagc agaaaatgtt gctcttccag
     1681 atgaggaagt gcttcatcaa ttactttgcg aaatgctttt tcgagtgtat gcatagttcc
     1741 tcctcaggac gatgcgttca cggctttgat catcgcttcg gccttgagca gacattcgtc
     1801 gtactccctt tccggatcgg atgctgccgt gatggcgctg cctactgaaa aggaaagata
     1861 gcgtttttgc cgatcataaa aaagtgaacg gatcaccaca ttgagatcgc aatcgccatt
     1921 ggctttgcgg aaaccaacag atcccgaata ccatccgcga cgcgattgtt cataacgggc
     1981 aatcagctcc atcgctttca ccttgggagc gcctgtcatg gagcccatcg gaaaagcagc
     2041 cttcaatata tcgtaagtgt taactccctc gcgtaactca caggaaatcg tggaaatcat
     2101 ctgatgcaca gttgggaaag tgtacacctc catgagttca tccaccctaa cactcgaccg
     2161 gatggcaata cgcgaaagat cattgcgaac aagatcaacg atcattacat tttcacttcg
     2221 ctctttcgaa tcattgcgca attgctcacg cagcacctta tcttcctcct gcgatttccc
     2281 tctgcgaatg gtgcctttaa tgggttgcga cagtaagcga tcgcccttgc gttgtacaaa
     2341 acgctccgga ctcccgcaga gtaaaaattg ttcctccctg cgcaaaaaag cagaaaaagg
     2401 agcgcgggta attgaatgca aattggcaaa cactacaaaa ggatctactt ccacttcctc
     2461 cgcaaaaaac tccatgcaaa aattcacttc gtaaatatct cccttacgaa tatgttcctg
     2521 caaactcaaa acagattgca ggtattcttc cttgctgatg cgaccgtgaa aatttattct
     2581 tttctgcttt tcaattgcag gaactaaaaa atcaccaagc ggtggtaaag caatcgttgt
     2641 tgcaggatgc atccacataa tcgaaggaac atccattccc ttatctgacc cgcactgaat
     2701 attttcaaaa gccgtattca gttcgtaagc aagtgcaagt acaatatcac cttccggaat
     2761 tttttccacc ggaccactct cccatttttt tccgctgtaa aaaaccggtt gtgcatcagg
     2821 accacaattc agtagtaaaa aattttcttc actaagggaa agttgtgcat accattcgga
     2881 aaaagaggaa acagactcca tcatgattgc atcgccagat cacgcaggca ttctacccag
     2941 gtgggatgat cgttcagact atttaccaat tgcaaatgtt cgccgccatg ttcttcaaac
     3001 atttccttgt aggcttcacc gatctcaacg gtggtttcga gacaatccgc cacaaatgcc
     3061 ggagagaaaa caagcaaacg cttcactcca ttttttgcga gttgctcaat ggttttatcg
     3121 gcatacggct ccaaccaggg gtcccgtccg agccgcgatt ggaagactac ggtgtattcg
     3181 gaaatattca attcggcagc aatgcgtttg gccgtattgt aacaggttgc tttgtagcaa
     3241 aatttatttt cgtcggtgat gccttcttcg caatggtgat ccttgcacgg acgattatcg
     3301 aaatacactt tatcgacatg tcgctcggga actccgtgaa aagaaaatac gacatgatcg
     3361 tacgatttca aatcatgttc acgtccccgc tccacaaaag cctgaatgta tttggggtga
     3421 tcgtaatact gactaatcat tttaatttcc ggaatcaccc accatttgct gatgatctgc
     3481 atcgcttttt cgagcgaaga tcccgttgtg gaagaagcat tctgcggata cagtgggaga
     3541 ataataatgc gatcgtaatt ggcgcgactc attttctcca gcacactttg catggatgga
     3601 ttctgatacc gcatcgcaaa ctcaaccgaa taattttccg gaagaatttt ttgcagatcc
     3661 tccttcacct ttaagccgtg caacaacaaa ggcgatccct gaggcgtcca tagttttcta
     3721 tattccttcg ccgacttcgg cgcacgaaaa ggtgctatga tcaggttcac caacatccat
     3781 cgtccaacag gattgatgtc gatgacgcgc ccgtccatga gaaactctct caggtacttg
     3841 cgtacatcag aagttttggt ggaatcgggg gtgccgaggt tgaggaggag gatcgctgtt
     3901 ttcatttttt caccactaag gcactaagat cacttaggta cactaagtta ttttgggagt
     3961 tcgttgaaga gttcatttac cataggaaca agtccggact tggtaatgtt ctcagaaaag
     4021 aaatttatta ataatccttt gggttttcct gccagtttca tgtaagataa caactgcgcc
     4081 ttatgcaccg gaagaatcga ttcaactgct tttagctcga taattatcag atcttccacc
     4141 aaaacatcga gtttcaaagc agctcctgca tcctcgcctt tgtataaaac cggaactgtt
     4201 acctgacgtt ttacttcaaa tcctttggat tgcagttctt ttaccaggca gttttcatac
     4261 accgattcca acaaaccggg gcctagttca cgatgaacct caatggcaca gcccacaatc
     4321 gcataagccg tgtcattgac atattgctgc gtaatcctta gtgattcttt gtgcactgag
     4381 tgtcttagtg gttaaagaaa tcaaatatga agtgcccttt tccctgtagc atctaaggcc
     4441 gcttccttaa gcgcttcggt gaaggtgggg tgcgcgtggc agatgcgtgc gatgtcttcg
     4501 gcggaggcgc ggtattccat ggctacaacg gcttccatga tcatatcggc agcgcgtgga
     4561 ccgatcatgt gtacgccgag tacttcgtca gtttcttcat cggcaatcac ttttaccaat
     4621 ccgtcgagat ccatgcttgc acgggcgcga ccgctggctt tgaaggggaa ttgtccgatc
     4681 ttgatttttc ttcctgcttt tttgagttct tcctcagtct gaccaacagc tgcaacttcg
     4741 ggccaggtgt aaacaactcc gggaatcaag ttgtaattca tgtgcggttt ttgtcctgcc
     4801 atgtattcgg caacgaacac accttcttct tcggccttgt gagcaagcat tgcaccgcgt
     4861 accacatccc caatagcgta aatgttgggc acgttggttt gcaaatgatc atccacatgc
     4921 acgcgaccgc gttcatcgag tttaacacct gcattttcga gtccgagatt atcggtatac
     4981 ggacggcgtc ctactgaaac gagacagtaa tcgcctttga attcgacttt ttgtcctttg
     5041 gcatcttctg ctgtaacggt agcttctttg gcgctgcctt taacgctaag aactttatgc
     5101 tggaaataaa attcgaatcc gagttttttc agtgacttct gcagttcttt acccatagtg
     5161 ccatccatgg tgctgatgat ggaaggagcg tattcaatca cagaaacctt tgcaccgaga
     5221 cgcgcataca ccgaaccaag ttcaagtccg atcacaccgc caccgataac gatgaggtgc
     5281 ttgggaactt cattcagatt cagggcttcg gtagaagtga tcacacgctt cttgtcgatt
     5341 tccattccgg gaaatgcgga aggtttggaa ccggttgcga tcacgatctt ttttccttcg
     5401 atagtggtta ctttaccatc atcgcttttg atatccactt tggtgttaga ctggaaggag
     5461 ccatgtccgt tgtaaacatc aattttattt ttcttcatca ggaagcggat accgtctacc
     5521 gtttgtgaaa ccacaccgtt cttgcgttcg atcatttgct tgaggttcac cttgaggtct
     5581 tttacatcaa taccgtgctc cttgaaggtg tgactcgcat tatgataatg ttccgacgaa
     5641 tcgaggagtg ctttggaagg aatacaaccc acattgaggc aggtgcctcc gagggaattg
     5701 tagcgttcaa ccaatgcggt tttcattccg agttgcgcgc agcgaatagc acaaacatat
     5761 ccgccgggac cggaaccaat aatgacaacg tcgtaattgc tcatgatgaa aggtttttat
     5821 ggaacgaaag tacgcaatag gcagcaatgc cggaagaagt tccggtaaac gattgaaaat
     5881 aagtggtttt tcatgggggc acaggatagt acaagtggct tgaaaaagcg gctcggtaag
     5941 atccatgctc tttgcgccta ttgcttcacc caacgtgcaa aatatacttt tccctcttga
     6001 tcgtgtagct taatgaggta atggccgttg gataaatagc caagctccag atgcgtttgg
     6061 cttcccgaag ttgaagttga ctttactaat cttcctgaaa catccaaaat ctgaatttct
     6121 cccatgaggt gatcatgaac aatcgttaac ctgccatcag aaggattggg aaatagatgt
     6181 agaccaactg caatttcttg attttcggtg gataaaaaag attctgcttc gggattcatt
     6241 tctgcctgtt gatttccgct tacgtccgcg gcccgaggat tcaaacagcc atttggtgtg
     6301 ccatccagca acgtgatgaa accggtataa tcatgaacca cattattaca atcgattaaa
     6361 ttgaggatat aaaaataggt gccatcactt aaacaaggag atcctggatt acacgccggc
     6421 catccctgat tgcatattcc gtcccaaatt ttaaaatcaa aggaattgac tatatccgtt
     6481 gcaaaataaa cacgctggtt aatgtcgcgc acttcaactt catagcgttt aactccggtt
     6541 gcctgaactc tccaggaatc attaactcca tcagaatttg gagttattac gttagggaga
     6601 acaaccaggg aaaaggtcgg atcattgttc caaagaaaac tctgcgttgc caaatttgca
     6661 tcgcatttgt tgtttacctg aacagatacc tgataatctt tgcaaggatc aatgagcaga
     6721 cgcgttgcat cactgaaatc aaaattggag aaggaaaaac tgcaacaatc aaaatctgag
     6781 tccgcataaa agcattctgt tgcgatgact tgtcctgttg acacatcggt gagtgatata
     6841 caaaccttct ctgtagatgg gcctacctga gcatccatag agaataccat atgcgattgc
     6901 tgaatattac tcaatgaaag gaccgggttc ggatcaggat tgtcattgta gtaaatggac
     6961 accggataat tcaattgttg ctcaaatgaa aatggaggat taaataaata accacatacc
     7021 gaagttactg ttagcgtata attcattgta cctacgccgg aaggggctgt gaacgttgga
     7081 tttggagaat tggggttgtc cagataggtg gcagggctcc aggaaaatag caaaccaaca
     7141 tcaggcggac ttgttggtcc aagaacaaca gtggtatttc cgcacaattc taaggactgc
     7201 ggaataaaag gcggggcagg acaaggattg ttttcgcaag ggccaattac tgcctcaaaa
     7261 tagcaaccgg gttcggtaga aaaaccgggt tcgagcacta cctgattgcc ggcgcggtag
     7321 atgatgttgg aattgtattt tacaacgaca ggaccatcaa ttgtatgttg gttaacagtt
     7381 aaaccagcat acaaatcaat gtttgcttga tagatgttgg tctcattcca ataaatagta
     7441 ttttccactg ccctaatatc tgcgcatgga tcctcttcaa atagttgaac atcatctata
     7501 agtaattttg tatatacagc acctcctgaa tcatcacaca accgcaatat taaattacta
     7561 agattactaa aattatctcc catcggagca ataaatgttc tgacttcctc aacccattca
     7621 aaagtagacg ttgtccaaac taaatttgcg ttaattgcct cccacttttg attattattt
     7681 gaatttgaat tccagtgctc accccatttg gtaaaaaaaa atcttaggca accagaccca
     7741 ctttctaatt tatacttata ctttactaca taccgcctac ccccgattag actataagga
     7801 attccatttt taactaatcc gatcctcaga ttctctattt ctgtggccat atgaacaaat
     7861 ctcgttgaat tgcctggaaa tacatactca ttacaagaag ggtcaatcca atcaggagta
     7921 ccattgtcgc ccaaaaaacc agggtttaat tgtgctactt tccacccata aagatcattg
     7981 ttaaaattat tcattccttg aacatgaatt gaaccaccag ggctgtaatg attactacac
     8041 cctggaacgg ctccattatt tccaaattca aatccggggt ttggaacaag atttggagca
     8101 tttggatctg tctggcaaac accgagctgt ccaattatga tgaacaatac tgaaattaca
     8161 agataaatta ttttcatttt ttatagttaa ggttttttcc tattgaaaca cctccgtatg
     8221 gatagtaatt atttacaaaa tcagaaaaaa cataaaagct aaatccagta aaaattccgc
     8281 ttgaaaaaga atatttatac cccaaagatc catataaata aaaatatggt aatttactac
     8341 actgcccgtt ataattagct gaacaagaat aattatctaa tttaaaaatt gctcgtatat
     8401 caggcaacaa taaaaatccc aatcccacaa aaaaacttga tttcttcctt tttaaaaaag
     8461 agcgattagc ttcggtaatt aaaatgattt ctgatcctgg gtctgaagaa tttattaaat
     8521 aatcgtagta ataagaaatg ccgaatcggt accggttaaa ttgtttttta ccaaaactag
     8581 ccgcaaaatt taacgaatac aaatagcctg gcccaaatcc ttctgcaaaa atttcccttt
     8641 gcttagtatc att
//
LOCUS       DAOMYG010000074         8628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2725_length_8628_cov_10.868657, whole genome shotgun sequence.
ACCESSION   DAOMYG010000074 DAOMYG010000000
VERSION     DAOMYG010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8628
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2725_length_8628_cov_10.868657"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2589)
                     /locus_tag="PK610_03705"
     CDS             complement(<1..2589)
                     /locus_tag="PK610_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HRJ35062.1"
                     /translation="MKKLYFLFTLFFSTASVQAQVAPLVNTTWNQGCYYNADCPTTGS
                     GGACGRVWTGCGATAYGQILKFYSFPVTGNGSHCNSTAPTHCVDFSAQSYNYSVMPAA
                     LSAHNSEVAKLLYHAGISLDMSWSGSVSLSGASQMALKKYYNYSLSIRGLLKALYTNT
                     EWENKVRSELDAGRPVFASGGGHFYIIDGYQLSPLRFHVNFGWGGLYDGMYNIHSIIA
                     GGNDYTPGNLIVGIQPRTQAVETTTDTLTVTAGGGNVPFEVGALAAWTITSNQGWAMP
                     NLTAGIAGYYSFNEGATVNIGTNATYIPRYATITATSGVATSSFVIQQSGILPSLSVN
                     PSLLTFSSLAGNQTFAISSDSIWTASSPDSWISITPNTGNGNLSVTVDVTANGPVART
                     GTVIVTRGNLQRTLSIEQSSNSSFWCTPALVISGTNGLTNVTLNTINRTSSNNEGYIH
                     TGLSTTLKRDSTYTFSATFTGSVAPAIWIDWNIDGDFNDAGEAIMPPAGSWYPSFTGT
                     KSLNFTVPSTAVEGTTRMRVYVKNFPSPTTSPCNTTDQGGDIEDYDIVVADHRNLDVN
                     PVALNFIASGQLQNVTVSSDSIWQVTCPAAWINLSAASGSGNGTLGITALANTATTPR
                     SALATFTRGFRTKTVLLEQQGEDSLLNIIETTLSIPATGNTYVVNVISNVDYSITSSA
                     AWITTNTASGSGISSFDVNVGLNALTTSQSGYVVVRSGTNSDTLWITQDANAATLGVS
                     PSLINFPETASDQLVSITTSGSWIASSNDAWLTIDINSGSGNGSLTVFAEENTGIART
                     GSITVTNGIINEVIVVNQSGVNGVTDFTTNEKPMVFPNPARDIITILSPDFMNNSRIE
                     IYSADGK"
     gene            complement(2602..3267)
                     /locus_tag="PK610_03710"
     CDS             complement(2602..3267)
                     /locus_tag="PK610_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ35063.1"
                     /translation="MKRISLLLVLTFGLYCNAFAQVRLSVISAPGSPDTTLMFQQVTY
                     LVVTKNTGNVTHFGSYQINIGVWDSVNQLPVIVHTQNQQPSSGSIAPNGNDTMLVVHS
                     IDSAAYRAGGNTIVIWPTPPTGTIVDTLYKQTYVIVFNDLSDLSSEPILIYPNPATER
                     FFIQSENPIEQVRIFDLNGRLILNSGKSPFVPTYELQTGTYLVEIELVTGAKRHSKIV
                     IQH"
     gene            3348..4811
                     /locus_tag="PK610_03715"
     CDS             3348..4811
                     /locus_tag="PK610_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35064.1"
                     /translation="MESALNRLVNSLDKEEIRHFKLFAGKYASDADRKDLSLFDYIRK
                     SGERYQEERIVKLLYASENKNSFYRLRNRLISEVSKSLLVQHYDEGDLNVVLYYLILS
                     RIFQQKQDYKNAWFYLRRAEKKAEAASAHDLLESVYGEIIRLSSEWLEVDPEDYIRKR
                     AANREVLSGIQQIDDILAVLTYRIKVSQNFSKGNLKVTRLLDATIREFSANKELAASP
                     VFRMKVYQSVSRILLQEHNYIELERYLMETYAAFTREKLFSRQNHDVKLQMLTYLVNA
                     LFKNKKYKDSLRYAEELGKATDDFQQLLKEKYMVYYYNALINNYAVLNKERAIEIILK
                     AQQERSIAKNKFYRIFLDLQLAMQYFDLKEFKRSSKLLVKVRHEENFKLFDAVFQLKI
                     VIMELMVRYELGDTDIVEREIPRTRKVFSEQLEDTANSRQSLMLDFLKKAISASGLRN
                     DTGLRKISIQLIDDEGYADSSDADLIDYKEWLKHKGF"
     gene            4980..6155
                     /locus_tag="PK610_03720"
     CDS             4980..6155
                     /locus_tag="PK610_03720"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ35065.1"
                     /translation="MKKLLLTLAVVLGGLSAFAQNFTFSGTITETGTGNPVANHTVTV
                     MADSSVMNFTGTAVTDASGNYTIVIPNGAQIGPNVNYWAWTDSCNGYASFTFQNMQGT
                     VNSATWNISVCGASTSCYATFTYQDSSSTGTIYFAPDNTNPAYTYAWSFGDGATSSLA
                     YPIHYYSAPGQYNVCLTVSNGTCTVTECSWIVVNPTNNACTANFWVYADSLNNTVYLV
                     NNSSWNANMQYVWDFGDGNTGTGQYPSHTYANYGTYLVCLTIADGAGCTDTYCQSVTF
                     QPFMNDDNSRSGFTLVVIAPTVLTVEEENAVTEFTAYPNPASEIITVDITSNINGNVS
                     IQVFDMLGKNAMEVPSAINAGNNRIELNLSTLQNGMYLLSVRSADVSSLQTIRIIKQ"
     gene            complement(6228..6953)
                     /locus_tag="PK610_03725"
     CDS             complement(6228..6953)
                     /locus_tag="PK610_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRJ35066.1"
                     /translation="MTESAKIFDDIVNDRRAVRKFHPTDYDPEAITRSLQRSRLAPSS
                     SNMQLYELYRVSDPEKLKLLSHYCMDQNTTETAKEMIVVVARPDLWKRRAKSNYDFVV
                     KHYANRESHKGMSTMTYFKKLMPLLYNNDIFGIRGTLKKLFVQVIALRKPMVRQVNGT
                     DIRIVVHKSAALVAQTFMLSMSAEGHDTCPVEGFDSLRVKKLLGLPWRAEINMIITCG
                     KRIPEGIYGERFRIPESEVMFTI"
     gene            complement(7073..7936)
                     /locus_tag="PK610_03730"
     CDS             complement(7073..7936)
                     /locus_tag="PK610_03730"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRJ35067.1"
                     /translation="MNTRLIITGLFALSAFTSILAQEPPAPPTPPAPPVPAGNEEKPD
                     TTQFDLGNMRVIMYEKSKSEKVEISDKKEDKEDSDHDFGVWSGFGLGVNAFMTYDNKI
                     GVPENANFLELDYARSMTVNFNFAEKRMRIIKDYLSINTGMGIQWNRYGIKNNYEIRY
                     NSDSIYGVQNSQINYTKNVLKATYIQIPLLLEINTSKNADKSFHIAAGVVGGYKLGSR
                     LKTRWEDEGRDFKSKSKGHYFFNPFQAYLHGSIGYGDVSLFVNYGLTRVFEKNKGPQV
                     YPVTGGILFHF"
     gene            complement(8167..>8628)
                     /locus_tag="PK610_03735"
     CDS             complement(8167..>8628)
                     /locus_tag="PK610_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35068.1"
                     /translation="QAGFSSGCTDVVQFELRHKKKFRKEIISIPQEIVEQQLPDVQDS
                     ATFAPGIQIQYANNSGDSLNNRTPDQEEESVAYAAPSDRLTIREFIQRKANRKLFGKE
                     TVTREEVNASIAQQASRLTGMDISYRSAQEQELKEVQFSVGNFGFSRKAVK"
BASE COUNT         2335 a         1740 c         1948 g         2605 t
ORIGIN      
        1 tttgccgtca gccgagtaaa tttctatccg tgaattgttc atgaaatcgg gagaaagaat
       61 ggtgataatg tcgcgtgcgg gattggggaa aaccattggt ttttcattgg tagtgaaatc
      121 ggtcactcca ttcactccgc tttggttgac aacgattacc tcattgataa ttccattggt
      181 aacagttata ctgcctgtgc gtgcgatgcc agtgttttct tctgcgaata cggtaagcga
      241 accatttcct gatccggagt taatatcgat cgttaaccat gcatcatttg agctcgcgat
      301 ccaggaaccg gatgtggtga ttgacaccaa ttgatcggat gctgtttcag gaaagttgat
      361 cagcgatggg ctaacaccca atgttgctgc attcgcatct tgagtgatcc ataacgtatc
      421 ggaatttgtt ccggatcgaa ccactacata accactttgt gatgtggtaa gtgcattgag
      481 tcctacgttc acatcaaaag aactgattcc gcttccactt gcagtattgg tggtaatcca
      541 tgccgcggat gaagtgatgg aataatcaac gttggaaatt acattcacaa cataagtgtt
      601 tcctgttgcc gggatgctta gggtggtttc tattatattc aacagactat cttctccctg
      661 ttgttccagc agaactgttt tcgttctgaa accgcgcgta aatgttgcaa gtgcgctgcg
      721 tggagttgtg gcggtatttg caagtgcggt aataccaagc gttccattgc ctgatcctga
      781 tgctgctgaa agattaatcc atgcagcagg acaagttacc tgccagatgc tgtcggaact
      841 tacagtaaca ttttgcaatt gtccggatgc aatgaagttg agtgcaaccg gatttacatc
      901 cagattacgg tgatcagcca caacgatatc atagtcttca atatctcctc cctgatctgt
      961 tgtgttgcat ggacttgtag ttggactcgg aaagtttttt acgtatacac gcattctggt
     1021 agtcccctca acagcggtgg agggaacagt gaaattaagt gatttcgttc cagtgaaaga
     1081 agggtaccac gatcctgccg gtggcataat ggcttctccc gcgtcgttaa agtcaccatc
     1141 aatattccag tcgatccata ttgcaggagc aacacttccg gtgaatgttg ctgagaaggt
     1201 gtaggtagaa tcgcgtttga gtgttgttga taatccggta tggatatacc cttcgttatt
     1261 ggaggacgtt ctgttaatag tgtttaatgt aacattggtt aatccgtttg ttccgctgat
     1321 tacaagtgca ggtgtgcacc aaaaactgct gttactcgac tgctcgatgg atagtgtgcg
     1381 ttgaaggttt cctctggtaa caatcaccgt tcctgttctt gcaacaggtc cgttggcagt
     1441 tacatccact gtaacactca aatttccgtt tccggtgtta ggtgtaatgc taatccagga
     1501 atcaggactg gaggctgtcc agatactatc tgaactaatt gcaaaagttt ggtttcctgc
     1561 cagtgaagaa aatgtcagca aagaaggatt tacagaaaga gatggaagaa ttccgctttg
     1621 ctgaatcaca aatgaagatg tagcaacacc tgaagttgct gtaatggttg catagcgggg
     1681 aatgtaagta gcatttgtgc caatattcac agttgcgcct tcattgaaac tgtagtatcc
     1741 tgctattcct gcagtgagat ttggcattgc ccatccctgg ttggaagtga tggtccacgc
     1801 tgccaacgca ccaacttcaa acggaacatt tccaccaccg gctgttactg ttagagtatc
     1861 ggttgttgtt tctactgcct gcgttctagg ttggatacct acaataagat ttccgggtgt
     1921 ataatcattt cctccggcaa taattgaatg aatgttatac atcccatcgt acaatccacc
     1981 ccaaccgaaa ttaacgtgaa aacggagggg gcttaattga taaccatcta taatataaaa
     2041 gtgaccgccg ccacttgcga atacggggcg gcccgcatcc agttcgcttc tcactttgtt
     2101 ttcccattcg gtattcgtat acagagcttt cagtaatccc cttatggaaa gcgaataatt
     2161 gtaatatttt ttcaatgcca tttgcgacgc accactgagt gatacgctac cgctccacga
     2221 catatcgagc gaaattcctg cgtggtacaa aagttttgcg acttctgaat tgtgcgcact
     2281 tagagcagcc ggcattacac tgtagttata ggattgagca gagaaatcga cgcaatgagt
     2341 tggtgctgta gaattgcagt gacttccgtt tcctgttaca ggaaaagaat agaattttag
     2401 gatttgtccg tatgcagtgg caccacaacc ggtccatact ctaccgcatg cacctcctga
     2461 gcctgtggtt gggcaatcgg cattgtaata gcaaccttga ttccatgttg tgtttaccaa
     2521 tggcgcaacc tgtgcttgaa ccgatgctgt gctaaagaaa agggtgaaga ggaagtatag
     2581 ttttttcata atctgaaacc attaatgttg aatcactatt ttagaatgac gtttcgcgcc
     2641 ggtaaccagt tcaatttcga caaggtatgt gcctgtttgc aattcgtatg ttggcacaaa
     2701 aggacttttt ccggaattca ggatgagtcg accattcagg tcgaatattc ttacttgttc
     2761 aatggggttt tctgattgaa tgaaaaagcg ttcagttgcc ggattggggt aaattagaat
     2821 aggctctgag gacagatcag aaaggtcatt aaacacaatc acataggttt gtttgtataa
     2881 agtatccaca atggtgcccg taggtggagt tggccaaata acgatggtgt tacctcccgc
     2941 acgataggcg gctgagtcga tggagtgcac caccagcatg gtgtcgttcc catttggcgc
     3001 gatgctgccg gatgagggtt gttggttttg tgtgtgcaca attaccggga gctggtttac
     3061 cgagtcccat accccaatat tgatctggta ggatccaaaa tgagtgacat tacccgtgtt
     3121 ttttgtcacc acaaggtaag ttacctgttg gaacataagc gtggtatcgg gggagcccgg
     3181 cgcgctgatc acgctcaggc gtacctgtgc aaaggcgttg caatacagac caaaagtcag
     3241 gacaagaagg agggaaatgc gtttcatggt aattcgcttt gtacaagatt acggcgaatt
     3301 attgatcggg ttgccttaac tttggataaa tcttacgcat tcaaactatg gagagcgcac
     3361 taaatcgttt ggttaattct ttggataaag aggaaatccg acactttaaa ttgtttgcag
     3421 gtaaatatgc cagcgatgca gatcggaagg atctaagctt atttgattat atccgaaaat
     3481 cgggtgagcg ttatcaggaa gaacgcatcg taaagttgtt atatgcaagt gaaaacaaga
     3541 acagttttta ccgactgcga aaccgattga tttcggaggt cagtaaaagt ttactggtac
     3601 aacattatga tgaaggagac ctgaacgtgg tattgtacta tttgattcta tcccgtatct
     3661 tccagcaaaa gcaggattat aaaaacgcat ggttttattt gcgcagagca gaaaagaaag
     3721 cggaagcagc cagtgcgcat gatcttctcg aatcagttta tggcgagatc atccgtttgt
     3781 catccgaatg gctcgaagtg gatccggaag attacatccg taaacgtgcg gccaatcgcg
     3841 aagtactttc tggaatacaa cagattgatg atattttagc agttctcact taccgaataa
     3901 aggtttcaca gaatttttcg aagggaaatc taaaagtgac ccgtttactt gacgcaacta
     3961 tccgcgagtt ttctgcgaac aaagaactag ctgcaagtcc ggttttccga atgaaagtgt
     4021 atcagtcggt cagccgtatt ttattacagg agcacaatta catagagctg gaacgctatc
     4081 tgatggaaac ctatgcagca tttaccagag aaaaattgtt ttcgcgtcag aatcatgatg
     4141 tgaaattgca gatgttgaca tatctcgtaa acgcattgtt caagaataaa aagtataaag
     4201 attcgcttcg ttacgctgag gaattgggaa aagcaacaga cgattttcag caattgctaa
     4261 aagagaagta catggtatat tattacaatg cgctcattaa taattatgct gtgctcaaca
     4321 aagaacgcgc catcgaaatc attctcaaag cacaacaaga acgatccatt gcaaagaata
     4381 aattttaccg tattttcctc gatcttcagc tggccatgca gtatttcgat ctgaaagagt
     4441 tcaagcgttc atccaaacta ttggtaaaag tccgtcacga agagaatttc aaattgttcg
     4501 atgcggtgtt tcagcttaag attgtgatca tggaacttat ggtgcgctat gagttgggtg
     4561 ataccgatat tgtagaacgc gaaatacccc gcacgcgcaa agtgttttcc gaacaactcg
     4621 aggatacggc aaattcccgc cagtcgctga tgcttgattt cctgaaaaaa gcaatctctg
     4681 cctccggttt gagaaatgat accggtttgc gtaaaatatc aatacaactt attgatgatg
     4741 agggttatgc ggattcgtca gatgcagatt taatcgatta caaagagtgg ttgaaacaca
     4801 aaggatttta aggtgaatga gtatgatatt aagtcatttt gtatatataa atagttgtta
     4861 ttggttaatt tgaatgagta agaaattgct aaaacactct ttttttacct atgaaaccga
     4921 tacatctttg tactgtccaa atgggacaaa ctaaatctga acacttaaaa cctatactta
     4981 tgaaaaagct cttactcaca ttggcagtcg ttcttggtgg actatctgcc ttcgctcaga
     5041 actttacgtt ctcgggcact attactgaaa caggaacagg aaatcctgtg gccaaccata
     5101 ctgttacagt tatggcagat tcttcggtaa tgaattttac tgggactgcg gttactgacg
     5161 catccggaaa ttacacgatt gtaattccga acggagcaca aattggtccg aatgtaaatt
     5221 actgggcatg gacagattcc tgcaatggtt atgcttcttt cacctttcag aatatgcagg
     5281 gaacagtaaa cagtgctacc tggaatattt ctgtgtgtgg tgcaagtact tcttgttatg
     5341 caacttttac ctatcaggat tcttcaagta caggaactat ttattttgct cctgataata
     5401 caaatcctgc atatacatac gcatggtcat ttggtgatgg tgcaacaagc tcattggcat
     5461 atcctattca ttactattct gcacccggac aatacaatgt ttgtcttact gtaagtaacg
     5521 gaacctgtac tgtaaccgag tgcagctgga tcgtggttaa tccaaccaac aatgcatgta
     5581 ctgctaattt ctgggtgtat gccgattcgc tgaacaacac agtatatctg gtgaataatt
     5641 cttcatggaa tgcgaatatg cagtacgtat gggattttgg tgatggaaat acaggcaccg
     5701 gtcaatatcc ttcacatacc tacgcgaact atggaacgta tcttgtttgt ttaacgattg
     5761 ccgatggtgc aggttgcacg gatacctatt gtcagtctgt taccttccag cctttcatga
     5821 atgatgacaa cagtcgttcc ggatttacac tggtagttat tgctccaact gtgttgactg
     5881 tagaagaaga aaatgctgta actgaattca cagcatatcc taatcctgca agcgaaatca
     5941 tcacggttga tattaccagt aatataaatg gtaatgtttc aatacaggtt tttgatatgc
     6001 ttggaaagaa tgcaatggaa gttccctcag cgatcaacgc cggaaacaac cgaattgaac
     6061 tgaatctctc aacacttcaa aacggaatgt atcttttaag tgtgcgttca gcagatgttt
     6121 caagccttca aactattcgt atcatcaaac aataaccgca ttactgcttg ctggaaaggg
     6181 cctcttcgga ggctcttttt ttttgttttg tagggaaaaa tctaatttca aattgtaaac
     6241 ataacttcgc tttccggtat cctgaatcgt tcgccataga tcccttcggg aattcgtttt
     6301 ccacaggtaa tgatcatgtt gatctctgct ctccagggta gtcccaataa tttttttacg
     6361 cgtaaagaat caaatccctc aacaggacaa gtatcgtgtc cttctgcact catcgacagc
     6421 ataaatgttt gcgcaaccaa agcagcgctc ttatgtacta caatgcgaat atcggttcca
     6481 ttcacttgtc ggaccatggg tttacgcagg gcaatgactt gcacgaatag ttttttcagt
     6541 gttcctctaa ttccgaaaat gtcgttgtta taaagcaaag gcatcagctt tttgaaatag
     6601 gtcatggtac tcatcccctt atgactttcc ctgttggcat aatgcttcac tacaaaatcg
     6661 taattgcttt tggcgcgacg tttccagaga tcaggccgtg caaccactac aatcatttcc
     6721 tttgccgttt cagttgtgtt ttgatccata caataatgtg aaagcagttt tagtttttca
     6781 ggatcgctga ccctgtagag ttcgtataac tgcatgttgc tgctgcttgg cgctaagcgt
     6841 gatcgttgca agcttcgtgt gattgcttca ggatcgtaat cggtagggtg aaattttcgg
     6901 actgctcgac gatcgtttac tatgtcatca aagatttttg cagattcggt catggggcct
     6961 gttttattaa taggacgaat ataaacgcaa aatagtgtaa aagaaaaacc ccgggtttcc
     7021 ccggggcctt cctctctcta ctttgttgcg attatgaaaa gaacgaactt gcttagaagt
     7081 ggaacagaat tcctcctgtt actggatata cctgaggacc tttatttttt tcgaagacac
     7141 gggtaagacc gtagttcacg aaaagggata catcaccgta accaatgctt ccgtgcagat
     7201 aagcctgaaa ggggttgaag aagtagtgac cctttgattt cgatttgaag tcgcgtcctt
     7261 catcttccca acgggttttc agtcgcgagc ctaatttgta tccgcctacc acacctgctg
     7321 caatgtgaaa cgatttgtca gcgtttttgg aggtgtttat ttcaagtaat aatggaattt
     7381 gaatgtaggt tgcctttagg acattcttgg tataattgat ttgtgaattc tgaactccgt
     7441 aaatagaatc gctgttgtag cggatctcgt aattgttttt tattccgtac ctgttccatt
     7501 gaatgcccat tccggtattg atgctgaggt aatctttaat gatacgcatt ctcttttcag
     7561 cgaaattgaa gtttacagtc atgcttcgtg cgtaatccag ttcgaggaag ttggcatttt
     7621 cggggacacc aattttattg tcgtaggtca taaaggcatt tacgcctaat ccaaatcccg
     7681 accacacacc aaaatcatga tcgctatctt ctttatcttc ttttttatcg ctgatctcaa
     7741 ctttttctga ttttgatttt tcatacataa tcacacgcat gttgccaagg tcgaattggg
     7801 tggtgtctgg tttttcctca ttacctgcag gtaccggtgg cgccggaggt gttggtggag
     7861 ccgggggctc ctgtgcgagg atcgaagtga atgcactgag tgcaaaaagg ccggtgataa
     7921 ttaatcgggt gttcatatcg ctttgtttta atgtgggacg ttcaatgatt ggattagtta
     7981 cagtttgttt ttgtttttat cgggtttttt ttggcgttta taagtaggac gctcaaggca
     8041 aggattagtt acagagctct gaattttttt tcggtattga agttgaatat agctttacag
     8101 aaaaagcaga ctttctggtg gataaatacc aaagcaatga tagagaagtt cgaaatgcta
     8161 aggagtttat tttactgcct tacgactgaa gccgaaattg ccaacgctaa actgaacttc
     8221 cttgagttct tgttcctgtg cacttcggta cgaaatatcc attccggtta gccgcgatgc
     8281 ctgctgtgca atggaagcat ttacttcttc gcgggtaact gtttccttgc caaaaagttt
     8341 tcgattggcc ttgcgttgaa tgaactcgcg aatagtaagt cggtctgagg gtgcagcata
     8401 tgcaacactt tcctcctcct gatccggtgt cctgttgttc aggctgtcgc ccgaattgtt
     8461 tgcatactga atttgaatac caggagcgaa agttgccgaa tcctgaacgt cgggaagttg
     8521 ttgttcgaca atttcctgcg gaattgaaat aatttctttc ctgaacttct ttttgtgccg
     8581 cagttcgaac tggacgacat cggtgcagcc gctgctgaat cccgcctg
//
LOCUS       DAOMYG010000075         8511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2777_length_8511_cov_7.674905, whole genome shotgun sequence.
ACCESSION   DAOMYG010000075 DAOMYG010000000
VERSION     DAOMYG010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8511
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2777_length_8511_cov_7.674905"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..549
                     /locus_tag="PK610_03740"
     CDS             172..549
                     /locus_tag="PK610_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRJ35069.1"
                     /translation="MDENQLSHIVIGKAIKIHKELGPGLLEGVYKECLAMSLEKEGLK
                     VEREKMLPVYFEDIKLTSAFRVDLLVDGKLLIELKSVNEIHDIHMAQTINYLRLGNLK
                     LGLLINFNVILLKDGIRRVVNKL"
     gene            888..1772
                     /gene="miaA"
                     /locus_tag="PK610_03745"
     CDS             888..1772
                     /gene="miaA"
                     /locus_tag="PK610_03745"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRJ35070.1"
                     /translation="MKKTLIVVLGPTASGKTALSIALARKYKTEIVSADSRQVFREMT
                     IGTARPTEQEWDGVQHHLLGHLSIHDNYNAALYERDATKILNAIFEKHDNAIVCGGTG
                     LYIKALLEGFDEVPPADETIRQQLETDLSEKGIEYLQEELKKRDPDFFAIAEIQNPQR
                     LMRAIEMVMVSGKTHTELRKGKKKTLPWIVRKIGIDLPREELYMRINRRVDEMMQNGL
                     LEEAKTLFPYKHLNALQTVGYTELFDHLEGKIDLEKAVELIKQHTRNYAKRQLTWFRR
                     DEEIIWVKPGVVPDLGIE"
     gene            2042..3226
                     /locus_tag="PK610_03750"
     CDS             2042..3226
                     /locus_tag="PK610_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35071.1"
                     /translation="MDRHIVLLILAECCLQLINAAFMMILLIYMQKEGYADYEAAYFT
                     KFRFLSVLLFSIPIGYYIRGRKIKPLFYFACIVVPLSSLVLLYAVHVHYDTLIMLSQF
                     IWGMGFVGLQIAAVPFILRNSSPGTMTEGISLSHATWSIAGVVSGIMIYGLHRMDPIA
                     FNERNLLVMLALAGFAGLVFIRLIDIRENLNDPEDNSIDEKGHDWDKIGIASIPIIII
                     ATGAGLTIPFISLFFYNIHGIDSHSFSLIASLALSLVFFSALMVPQIKNKLGFRIAVP
                     LTQSLAIISLVALAYTENYKGTSAGLFAALGFYALRQPLMNMATPMTTEVTMKYVGKR
                     NQEMMSALTASIWSGSWFISAYLFEIMREMEVSYMNIFLITAALYSVGVIAYMLLMRK
                     IE"
     gene            3310..3978
                     /locus_tag="PK610_03755"
     CDS             3310..3978
                     /locus_tag="PK610_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35072.1"
                     /translation="MSRLLFLFFLISGNAFGQWNIDSAKRALKIYKLGSYSYHESFIG
                     KSDFAAPLILTRDGGAAKFGDDETESVKGGTLVKVDKRGKEQWKYFIKPQFSEIESQS
                     IIEDQKGNIYAFVLSYDHKRYRGGTQRVLKFDSKGKMMWDKTYGLYSVMKSPTFSSIT
                     MQPSGQLYIRGQMVTDVPASGKDPLYRYWEGWINGEGTLTQKTGEVIDWKAGKWKKWM
                     DQQE"
     gene            4121..5308
                     /locus_tag="PK610_03760"
     CDS             4121..5308
                     /locus_tag="PK610_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ35073.1"
                     /translation="MTPPRFLLLALAAIQFTNIIDFMIMMPLGKQIMHIFRIDPSQFG
                     VVVASYTLAAWVSGMVAALFLDRFDRKKTLLFTYTGFIIGTFFCAFAPSYELLVGSRI
                     LTGLFGGILGAQVMAIVADFYPYEKRGKAMGVIMASFSAGAALGVPIGIYIARHFTWQ
                     APFIAVAITGTVILFASIYILPSMRSHLVKPADRSAMFSLYKSAFTVSSQRNALLMMI
                     VLMLGHFMVVPNVATHLQFNLNFSENELTLVYLFGGIVSFFSNPFVGKLSDQIGKEKM
                     FTIMVLLAAVPVLLITQITNAGVPLMLCITTLFFAFSGGRFSPAQALVSEAVPPNLRG
                     SFMSFVSAMQQLGAGLGALIAGYLMWTTADGRLEGIGTVGIIAVLTGFGSLLFVNRIR
                     SGC"
     gene            5503..6375
                     /locus_tag="PK610_03765"
     CDS             5503..6375
                     /locus_tag="PK610_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018674460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HRJ35074.1"
                     /translation="MRQLKITKQVTNRETLSLDKYLQEIGRVELITAEEEVQLARRIR
                     EGDQRALEKLTKANLRFVVSVSKQYQNQGLTLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNKIGSINKINKAFARLEQTYERIPTDDELAE
                     VLEITPEEVKQSIRYSGRHVSMDAPLKEGDDDSSSMYDVLIGEDSPSPEGGLIKDSLR
                     REIERSLTTLTPREADVVRLYFGLNGEHPLTLEEIGERFDLTRERVRQIKEKAIRRLK
                     HTSRSKLLKAYLGQ"
     gene            6489..>8511
                     /locus_tag="PK610_03770"
     CDS             6489..>8511
                     /locus_tag="PK610_03770"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HRJ35075.1"
                     /translation="MRILTLLILLLQVFTLHAQNTQQILHPGGKYPLVGQWQKLEIGI
                     QLEKTIMEEIQLFLRNKNEGLNPFAEEDIKIVSTFTSESGSTRKMEAFYYQEYRADYA
                     RNKWIRESATHPFRVRFAPYEKGTWKVKTVISIRGKQESELDEFTFNVVASESKGRLL
                     LPEKNAPNPFILTTEADGKGFFAIGENITHSNYIDLDVISHERHKKWLRQLADQGANW
                     VRLEYGAQNFLIDWMDINDYSSRLDRAWAMDELVELCMERNLYFILFNHHVEYDAPEV
                     DPHWKDRPTQWKNNSFCKQLNLKSVTETFSSEDAWKYFKRRFRYMFSRYGYAPQFAVW
                     EFSELDNIFGKDSEGKNVYFNNRSTRDTFRKFFIQLKSYIQNDLGFSDKHIAVSFANN
                     PKTNNNNDHIYSVCDLTFIHKYGERKDENFAMRSRLHEEIRQRFRKPCMMEEMGPRDA
                     NIYCCSKAQFHHDIWSNAFMGGFGTGMHWWWDRGIHDLSYYEDYRAMVIFFQEEDLAK
                     KEMLPKKLSDKNGNSASARSKRKWECFYLRDKKGEDVLGWINNATSFWGNENTDCMNE
                     LRSKKYLSNPCKMEDGAALACPPPCAAYIDYGGKDFKDSYNEVIDLNDLILEIPDLSP
                     GRFFGNKTNYRVEWWSTDKKGGMLSAEVTSTNATGTLKIKVPAMSKTAKH"
BASE COUNT         2577 a         1776 c         1785 g         2373 t
ORIGIN      
        1 caatcttttt aaggggagca aagaatgcca taggcatacg cgaaggtgtc ctaatgaaaa
       61 tttatggaaa cacattgata attctgggtt taccgaaata caaatgtgag ctaaaattga
      121 tttgaatatt tcatgggtca tcatttcatt tgtattctat aatggtaatt tatggatgag
      181 aatcaattat cacatattgt aattggaaaa gcaattaaaa tacataagga attgggacct
      241 ggcttgttgg aaggggtgta taaagaatgt ttagcaatgt ccttggaaaa ggaagggctc
      301 aaggttgaac gggaaaaaat gcttcctgtt tatttcgaag atataaaatt gacatctgcc
      361 tttagagttg atttgcttgt tgatggcaaa ctgctcattg aactgaaaag tgtaaatgaa
      421 attcatgata tccacatggc tcaaaccatc aactatcttc gattaggcaa cttaaagctt
      481 gggctattaa ttaatttcaa tgttatcctc ctgaaagacg gaatacgaag ggtggtaaat
      541 aaactttgaa tgaatctaaa ttttccacaa aggttaattt tcacttacat ccaacaatct
      601 aatttgcgat tccaattttc actttttcac cttcgcgata caccaaaggt gtatttttac
      661 ggtcagccgc cagcccaacc gtcagcgtcg gggagtcgaa ggggacttca cttaaaaaga
      721 taatgatata caacgcattt cctggcgaac caaacacaag gtctatccaa gatcaataat
      781 taatcctttg cggttaaaac cggtatatag cacttcaaaa tttctccttt aagtttttgc
      841 aatatgtatg ttgcattcta attgaaattg ctcatttttg tcttcctgtg aaaaaaacac
      901 ttattgtagt tttaggtcct actgcttccg gaaagacagc actcagtatt gccttggcaa
      961 gaaagtacaa gactgaaatt gtttcggctg attcacgtca ggtattccgc gaaatgacca
     1021 ttggcacagc tcgtcctaca gaacaggaat gggatggtgt gcagcatcat ttacttgggc
     1081 acctttccat tcacgataac tacaatgctg cactttacga gagggatgcc acgaaaattc
     1141 tgaatgcaat ttttgaaaag catgacaatg ccattgtatg cggaggaacc ggattataca
     1201 tcaaagcact actggaagga tttgacgagg taccaccggc tgacgaaact attcgtcaac
     1261 aactggaaac tgatctcagt gaaaaaggaa ttgaatacct gcaggaagaa ttaaaaaaac
     1321 gtgatcctga ttttttcgca atcgcagaaa ttcaaaatcc acaacgatta atgcgtgcaa
     1381 ttgaaatggt tatggtaagc ggaaaaaccc ataccgaatt aagaaaaggg aaaaagaaaa
     1441 cactaccctg gatagttcga aaaataggaa tcgatcttcc acgggaagaa ctttacatgc
     1501 gaataaaccg gagggtagat gaaatgatgc aaaacggatt acttgaagaa gcaaaaacat
     1561 tatttcccta caaacacctt aatgcattgc aaacggttgg ctatactgag ctctttgatc
     1621 atttggaagg aaaaatcgac ctggaaaaag ccgttgaact tatcaaacaa cacacccgca
     1681 attacgccaa gcgacaactt acctggttca ggagagatga agaaatcatt tgggtaaaac
     1741 cgggagtagt tcctgattta ggaattgagt aagcaatctc caattatcca aataactctt
     1801 gcgaaacgat atctgagcga ggacggaatt agttcaagtt cattctaaca atttcgaatt
     1861 gtaattttaa tcccggggct tcgactcacc taggctgcat gccactcagc ctcttctgtt
     1921 tcagccgcga ggtctgagcg tagccgaaga ccaatcctct ttaaaaatac agtgcccaaa
     1981 aaaacttcta ccttcgcaag cacattcttc gattgaaatg ctgccgggaa attttaaaaa
     2041 catggatcgc cacatcgttc tgcttattct ggcagaatgt tgcctgcagc tgatcaatgc
     2101 ggcatttatg atgatcctgc tgatttacat gcagaaagaa ggttatgccg attatgaagc
     2161 ggcttacttt acaaaatttc gttttctaag tgtattgctt ttttccattc ctattggcta
     2221 ctacatcagg ggaagaaaaa taaaacccct tttctatttt gcttgtattg ttgttccgct
     2281 ctcctctctt gttcttttat acgcagttca tgttcattac gacaccctga tcatgctttc
     2341 gcaatttatt tggggaatgg gctttgtcgg attacaaatc gcagctgttc cattcatcct
     2401 gcgtaattca tcaccgggaa caatgacaga agggatttcc ctgagtcatg ccacatggag
     2461 cattgccgga gttgtgtcgg gaattatgat ttacggtctc caccgaatgg atccgattgc
     2521 atttaatgaa cgaaacctct tggtaatgct cgcactggca ggatttgccg gactggtttt
     2581 tatccgactg attgacatcc gggaaaattt aaatgatccg gaagacaatt ctatagatga
     2641 aaaaggtcac gactgggata aaattggaat tgcatccatt cccattatca tcattgctac
     2701 cggtgcaggt ttaacaattc cttttatttc tcttttcttt tacaatattc acggaatcga
     2761 ttcacactca ttttcactta ttgcatcact ggcactcagc cttgtttttt tcagtgcctt
     2821 gatggtgcct caaataaaaa acaaactcgg attccgaatt gcagttccgc ttacacaaag
     2881 tcttgccatc atttcgcttg ttgcactggc ctatacagaa aactacaaag gcaccagcgc
     2941 aggactattt gctgcattgg gtttttatgc cttgcgtcaa cccctgatga atatggcaac
     3001 acccatgaca acggaagtca cgatgaaata cgttggcaaa cgcaaccagg aaatgatgag
     3061 tgcattaacg gcctccattt ggagtggaag ttggttcata agtgcctatc tgttcgaaat
     3121 catgcgggaa atggaagtca gttacatgaa tattttcctc attaccgctg ccttatattc
     3181 tgttggtgtg attgcatata tgttattgat gcgaaaaatt gaataagcat tcctgcatta
     3241 aaaaagattt caaccaccgg taagctttgg tccatttatt gatacctttc cattcagcaa
     3301 acaacattta tgtcccgact tctttttctc ttttttctga tttccggcaa tgcatttggg
     3361 cagtggaaca tcgacagcgc taagcgtgca ttaaaaattt acaaactggg gtcgtattcc
     3421 tatcacgaat cgttcattgg aaaatcagat tttgctgctc ccctgattct aacacgagat
     3481 ggaggcgctg caaaattcgg agatgacgaa acagaatccg taaaaggtgg tacattggta
     3541 aaagtggata agcgcggtaa agagcaatgg aaatacttta tcaaaccaca attcagtgaa
     3601 atagaatccc aaagcatcat tgaagatcaa aagggaaaca tctatgcttt tgtattgagc
     3661 tatgatcaca aacgctacag aggaggaaca cagcgtgtct tgaagtttga ttcgaaagga
     3721 aaaatgatgt gggataaaac ctatggattg tattccgtta tgaaatctcc tacgttttca
     3781 tccatcacga tgcagccgag cgggcaactg tatattcgtg gtcagatggt aacagatgta
     3841 cctgcatccg gcaaagatcc cttgtaccgc tattgggaag gatggatcaa tggtgaagga
     3901 acactgacac agaaaacggg agaagtcatt gactggaaag ccggcaaatg gaaaaaatgg
     3961 atggatcaac aagaataata gcctgacccg tctcaacctc tggatttatc ctgctaaaat
     4021 aagactgcaa gtcattgaat ctcgtaatca atcatcggga aacgcattgg taaaaaagta
     4081 tatttgtcgc gctctgaaaa gccgcaattt ccaactttcg atgacacctc cccgattcct
     4141 tcttcttgct cttgctgcta ttcagttcac caatatcatc gacttcatga tcatgatgcc
     4201 attgggtaag cagatcatgc acatcttccg tatcgatcct tcccaatttg gtgttgtggt
     4261 agcatcctat acccttgcag cttgggtatc cggaatggtt gctgccttgt ttcttgatcg
     4321 tttcgaccgg aaaaagacct tgttgtttac ttataccgga ttcatcatcg gaactttttt
     4381 ctgcgcattt gcaccttcgt atgaattatt ggttggttca cgcatcttaa ccggactatt
     4441 cggaggtatt ctcggcgcac aggtgatggc gatagtagcc gatttttatc cctatgaaaa
     4501 aagggggaaa gcaatgggag tgataatggc gtccttctct gcaggagcag cattgggagt
     4561 acccatcgga atttatattg cacggcactt tacctggcag gcaccattta ttgcagtagc
     4621 gataacagga acagtgatcc tttttgccag catatatatt ttaccatcca tgcgatctca
     4681 tttggtaaaa cccgctgatc ggtctgccat gttttcgctc tacaaaagtg cttttactgt
     4741 ttcgtcccaa cgcaacgcat tgctaatgat gatagtttta atgctggggc attttatggt
     4801 agttcccaat gtggcgaccc atctgcaatt caatttgaat ttttcagaaa atgaacttac
     4861 gcttgtgtat ctctttggag gtatcgtatc cttcttctca aatccttttg tcggaaaatt
     4921 atcggatcaa atcggaaaag aaaaaatgtt caccatcatg gtacttcttg cagctgttcc
     4981 cgttttgctc atcacacaaa tcacaaatgc aggagtaccc ctgatgcttt gtatcaccac
     5041 gctctttttt gcattcagcg gaggacgttt ctctcctgcc caggcattgg tatcggaggc
     5101 agtacctcca aacctgagag gcagttttat gagtttcgta tcagccatgc agcaacttgg
     5161 tgccggatta ggcgcgctta tcgcaggata cctcatgtgg accaccgccg acggacgact
     5221 tgagggaatt ggaaccgttg gtattattgc tgttcttacc ggattcggca gccttctctt
     5281 tgtgaaccgc atacgttcag ggtgttaaaa aaaagtcctt gttaagctac aaacctattt
     5341 catcatcaga aaggtgggtt ttgaccgtat tttaagcgat tccaccccta attaacacag
     5401 cgttaataag ttcatgacat ccctttacct catttctgcg tctttcctgt aaccaatcag
     5461 aaaatggttc gtttgagtac tgtagcctaa aacctattct caatgaggca attaaaaata
     5521 acaaaacagg tcaccaaccg cgagacatta tcgctcgaca agtacttaca ggaaatcggc
     5581 cgcgtagagc tgattaccgc agaagaggaa gtccagcttg cacgtcgtat tcgcgaaggt
     5641 gatcagcgtg ctttggaaaa actaaccaag gccaatcttc gctttgtggt ttctgtatcg
     5701 aaacagtacc aaaatcaggg attaaccctt ccggatctta taaatgaagg aaatctcggt
     5761 ctgatcaaag ctgcacaacg tttcgatgaa acacgtggat tcaaattcat ctcgtatgct
     5821 gtttggtgga tcagacaatc cattcttcag gcactcgctg aacaatcgcg tattgttcgt
     5881 ttaccactga acaagatcgg atcgatcaat aaaatcaaca aggcatttgc tcgtcttgaa
     5941 cagacctatg agcggattcc aaccgacgat gaacttgctg aagttttgga gatcactcct
     6001 gaagaagtaa agcagtccat ccgctattcc ggcagacatg tgtcgatgga cgcaccactc
     6061 aaagaaggtg atgacgattc ttcaagcatg tatgatgtat tgatcggaga agacagtccc
     6121 tctcccgaag gcggactgat caaagattcc ttacgcagag aaattgaacg ttcacttacc
     6181 acacttactc cgcgtgaagc agatgttgtg cgcttgtatt ttggattgaa tggtgaacat
     6241 ccgctaaccc ttgaggaaat cggtgagcgt ttcgatctta cccgtgaacg tgtgcgccag
     6301 atcaaagaaa aagcgattcg tcgtttaaaa catacatcca gaagcaaatt gctaaaagcc
     6361 tatctcggtc agtaagctgc attatacaga tatttcgaaa gagcctccat gagaggctct
     6421 ttttttttcc tgaacaaaga aatccacatt caatgatcag agaggtacct tttccataaa
     6481 ttcgtgctat gagaattttg accctattga tcctgctgct tcaggttttc acattacatg
     6541 cacaaaacac tcagcaaatc ctgcatcccg gaggtaaata tcctctggtt ggacaatggc
     6601 agaaattaga gatagggata cagcttgaaa aaacaatcat ggaagaaatt cagttgtttc
     6661 tgcgtaacaa gaatgaaggt ttaaatccgt ttgccgaaga agacatcaaa atagtaagca
     6721 cattcacttc agaaagcgga agtactcgaa aaatggaagc tttctattac caggaataca
     6781 gagcagatta cgcacgaaac aaatggattc gggaatccgc tactcatccc ttcagagttc
     6841 gttttgcgcc atacgaaaag ggaacatgga aagtaaaaac agtgatctca atacgcggta
     6901 aacaagaatc ggaattagat gaattcacat tcaatgtagt tgcttccgaa tcgaaaggac
     6961 gtttattact acccgaaaaa aacgcgccca acccattcat cctgacaacc gaagcggatg
     7021 ggaaaggatt ttttgccatt ggagaaaaca ttacccattc caattacatc gacctggatg
     7081 ttatcagtca tgaacgccac aaaaaatggc tccgacaact tgcagatcag ggtgcaaatt
     7141 gggtgcgatt agaatatggt gcccaaaact ttttgatcga ctggatggat atcaacgatt
     7201 acagttcccg tctcgatcgt gcctgggcaa tggatgaact tgttgaatta tgtatggagc
     7261 ggaatttata tttcattctg ttcaatcacc atgtggaata cgacgcaccg gaagtcgatc
     7321 cgcattggaa ggaccgtcct acccaatgga aaaacaactc gttttgcaag caactaaatc
     7381 tgaaatcggt aacagaaaca ttctcctcag aagatgcttg gaaatatttc aaacgacgtt
     7441 tcaggtatat gttctcccgc tacggatatg cacctcagtt tgctgtttgg gaattttccg
     7501 aactcgataa tatttttggc aaggacagtg aaggaaaaaa tgtctacttc aacaacagat
     7561 ccacgaggga tactttccgg aaattcttta ttcagcttaa atcctatatt caaaatgatc
     7621 ttggttttag cgacaaacac attgccgtta gttttgccaa caatccaaaa acaaacaaca
     7681 acaacgatca tatttacagt gtgtgcgatc tcacctttat ccacaaatac ggagagcgga
     7741 aggatgaaaa tttcgcgatg cggtcgcgct tacatgaaga aattcgtcaa cgttttcgca
     7801 aaccctgcat gatggaagaa atgggtccgc gtgatgcaaa tatttattgc tgcagcaaag
     7861 cacaatttca tcacgacatc tggagcaatg ccttcatggg aggattcgga acaggaatgc
     7921 actggtggtg ggatcgtgga atccatgatc tgagttacta cgaagactac cgggccatgg
     7981 tcatcttttt tcaggaggaa gacttggcca aaaaagagat gttgccaaaa aaactttccg
     8041 acaaaaacgg aaattctgca tcagcaagga gcaaacgcaa atgggaatgt ttttacttac
     8101 gtgataaaaa aggcgaggat gttttgggtt ggatcaacaa cgccacttcg ttttggggga
     8161 atgaaaacac cgattgcatg aacgaacttc gttcaaagaa atacctttcg aatccctgca
     8221 aaatggaaga tggagcagca ctagcctgtc ctcctccctg cgctgcttat atcgattatg
     8281 gaggaaagga ttttaaagac agttataatg aagtaatcga cctgaatgat ctcattctgg
     8341 aaattccgga tttaagtccg ggaagatttt ttggaaacaa aacaaactac cgtgtcgaat
     8401 ggtggagcac cgataaaaag ggaggcatgt tgtcggccga agtgaccagc acaaatgcaa
     8461 caggaacgct caagatcaaa gttccggcga tgtcaaaaac tgccaaacat c
//
LOCUS       DAOMYG010000076         8494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2781_length_8494_cov_10.028913, whole genome shotgun sequence.
ACCESSION   DAOMYG010000076 DAOMYG010000000
VERSION     DAOMYG010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2781_length_8494_cov_10.028913"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PK610_03775"
     CDS             <1..667
                     /locus_tag="PK610_03775"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRJ35076.1"
                     /translation="ASGQFGDDIREIIDNSIGQWSISGSVEKNWTPGEYPAPTKLFIE
                     KPDAIQTGIRIGRRMVNKTHADFAGLQVLNTALGGYFGSRLMANIREDKGYTYGIGSA
                     VVSLEKEGYFFIATEVGADVAKDAVAEIYKEIQLLREKTIPVEELQMVKNYMLGSFLR
                     NSDGPFAMADRFKALHFYGLDYSHYDRFLNTVNTITAQEIKDLAIKYLNPGDMTEILA
                     GKS"
     gene            745..4074
                     /locus_tag="PK610_03780"
     CDS             745..4074
                     /locus_tag="PK610_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ35077.1"
                     /translation="MIHNFSINIRTALRKLFVLTGLLLLLPQYGNSTHLLGGDITWTC
                     DGTGNYVFQLRIFRDCSGPSISIAGQSLRVWNHPSITQIPLIFVSQSDNSPVCTQVAG
                     GPVQITCASGNPNAVEEYIFRSTPVNLNAVPPAQGFAFTWDAFSRPACDNLQNALIYG
                     LTLRSFMYSNGGANSNPCNDSSPQFTSSPQYAFCAGEAANFGQNAYDPDLDSVVFSWG
                     TPIDQIAAGTFNPPAFPIAVPYSPNYSITSPLPGTALNASNVPGQINPATGDISFTSF
                     TTGKFNIVVKADSYRNGVLVSEVYREFLMVIVNCGPNTAPSATPVFGGGTSWSGTFLA
                     GDLIQFNLVASDPELLQDGSPQSVIMTATGLQFGTNFTDPANGCINAPCATLSNTLPQ
                     TGVQGLSTDFNWQTTCDHIPAGATQSQYSFTFFLSDDYCSVPGANSFTANITLLAPPS
                     VPSPDIRCADVAPNGDVTLSWNPSADPQSIFDRYEIYNSGTLIATLPAIGTNSFTHVG
                     ADAHLGIRSYHIITYSDCGGIRTTYSDTISTMFLQVNNPANGTAQLVWNPLRNPSLPS
                     ASAVYEIYREFPLGTWTLINTVPATQHFYSDTITVCDDSLNYRIEISDALGCHSTSSI
                     DGKTFQDQIAPYIPVITSVTVDTTSGLANINWEVNAAGDTQGYIILQNQGGNWIIIDT
                     VYGVNNTTYEYALSLAGLTSESYGIAAFDSCFSGNPPTANTSAMGTFHSSIYLTTSLN
                     ICAQRVTLNWNPYINWTGGVANYEIYASENGGAFQLIGNTNATNFVHQNVNRLSTFCY
                     LVIAYENGTTERSLSNRACRFIAQPSQPAWNYLQTATVSGNNVEVRCFTDLSATVLGY
                     RIERTTDLSLPFDPIGTAVPSGNPVSFFDYSADVGSSSYYYRAIAIDSCGQDALVSNN
                     GKTILLTIDANSLRGIVTLQWNAYQGWDGFVTEYRIYRSSNGVFDPAPIATVLPGMLF
                     YEDDVSMFLESDGEICYYVEAVESLNSFGIAETSISNLACAVIEPIVWVPNAFMVGGV
                     NEIFIPVISFADYSNYQLRIFDRWGQIVFETNDRAQGWDGKINNQLGREGVYVWVLEF
                     ADGSGQLFEKKGFVTLLKAPK"
     gene            4071..4724
                     /locus_tag="PK610_03785"
     CDS             4071..4724
                     /locus_tag="PK610_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35078.1"
                     /translation="MRSVFLLLIFSSLFFSACRKNKELVVEIKGRITNSGDQSSAAGV
                     TVKVKFQEIGSGSISSAFKTIATTTTDASGNYSVSFEKPAAAEYRFELSSPRHFATEI
                     NESADAFSANESNTRNFELDPVSFFSVRLKNNSPVGVQDQIIFQNTSESYSCSICCGN
                     TPVIKQGMTVDTSFVCRRKGGRFIRFSWTVTKGGMNQFYTDSVITPNYDTTYRLISY"
     gene            4728..6740
                     /locus_tag="PK610_03790"
     CDS             4728..6740
                     /locus_tag="PK610_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35079.1"
                     /translation="MKAFYTVFLLVFISFSFSASAQDEKKKKFLKDPADQATMDSAEF
                     FYQEKNYHIAIGKYKRLCENFPDEKILHFRIGVCQLNMQEAMEEALVHFEKLDEKAFE
                     REQSLYYRARALMLNYRFEEAIPLFEKYKEQKWSTFEFRFLSDIYITNCKNGIELLKQ
                     PVEAKIENIGLPVNTSASEYVPLINSEGSVLIYTYRGPRSTGGIQRSTNRNSVEAGSY
                     DEDIFISNFDPATGAWSAPEPLPFPVNTKANDASVYLSDDGHELYMFRSVDGDEGSLY
                     VSHLEGTEWSSPEKLYGKINSPWWEGSISISADGRTAYFSSERPGGFGGKDIYVAQLQ
                     PDGSWGNIRNAGPKINTHKDDDSPFIHPSGQFITFSSQGHNSMGGYDIYRCDLTNDST
                     WSDPINMGFPINTPGDDIFFVITGDAQAAYYSSGKAGGYGKQDIYKIIPGIFGKQIRM
                     IRVKGVVTVDDKPAASEIRITYEYTGVTQGIYTGNSASGKYLINLPSEKELVIYFKAE
                     GFEPQLRKINTKDVDAFLETTIDVQFYTDEYLRKLKSNGDSLKTIATNDGTLKTNTSG
                     TDDRFSKFGTTRVEGLRFHVQIGAYNMPQNFNYSTVMKLGKVEKQKLDDNITRFVIGT
                     FDTWAEAKEFCRLVIKAGIIDAFVTAEYQGKRYHIHELVEKGIFQP"
     gene            complement(6741..7481)
                     /locus_tag="PK610_03795"
     CDS             complement(6741..7481)
                     /locus_tag="PK610_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35080.1"
                     /translation="MRPVFLFACSFFLNASAFAQFNNSSNTIQFSNFSNPIEPRAQVQ
                     QRSTLHVSQTNTSTNTNRSNTNRSNPVRPTQQVQQAQQQAQQQARQTNVQTNRSVVNT
                     SNTSVANNDWAVNTEQSKTNEVEVIEVQQMEMNSGLDNVNAENVNVFQTNKPSEQENE
                     EVAEISVSTRESRTFDLPKLHWDKPSSLNNSGNDDSSSETRATVAVKRKKGSHYSQTG
                     GSRHSSARKKTTSGKVKRSGGMMPCVGF"
     gene            complement(7575..>8494)
                     /locus_tag="PK610_03800"
     CDS             complement(7575..>8494)
                     /locus_tag="PK610_03800"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRJ35081.1"
                     /translation="VLSNFNSVFGTPYKVIRVPMVPSTSGNWPGAPFGNGAYRNYPNF
                     VFVNKTAIVPVYRTQYDTTAIRILTEALPGYNIVTIDADNSGANLISAGGVIHCITNL
                     IGAADPMLISHQNLPDTYDDQNPYLVDAYITHKSGIASANLYWSLTPGTGYQAVAMTN
                     SGNGHWVANIPAQAVGSHVYYYVEGTAVSGKTQVRPMPAPAGYFHFQVLGTPSNVDEV
                     IAMEMDNIFPNPASSVTCIPVQTNLTFSGSLKMYDMLGKEVTEIYSGSFPMGESRYFI
                     HAEKFPAGVYSVVLERNGGGRFVQQLIIR"
BASE COUNT         2425 a         1980 c         1832 g         2257 t
ORIGIN      
        1 cgcatccggt cagtttggcg acgatattcg cgagatcatc gacaatagta ttggtcagtg
       61 gagcatcagc ggatcagtag aaaaaaactg gactcccgga gaatatcctg ccccaacaaa
      121 attattcatc gaaaaacccg atgccatcca aaccggaatc cgcatcggaa gaagaatggt
      181 aaataaaaca catgccgatt ttgcaggatt gcaagtcctg aacactgcac tcggcggtta
      241 tttcggatca cgacttatgg cgaatatccg cgaagacaaa ggatacacgt acggcatcgg
      301 atcggcggtt gtttcgctgg agaaagaagg ttatttcttt atcgccaccg aagttggtgc
      361 ggatgtcgct aaagacgcag tagcagaaat ttacaaggaa attcagcttc ttcgtgaaaa
      421 aacgattccc gtcgaagagc tgcaaatggt taaaaactac atgctgggtt ccttccttcg
      481 caacagtgat ggtccctttg ccatggccga ccgtttcaag gccctgcatt tttacggttt
      541 ggactactcc cactacgatc gatttttaaa cacggtaaat acaattacag ctcaggaaat
      601 caaagactta gcgataaagt atttgaatcc cggcgacatg actgaaattc tggccggaaa
      661 atcatgattt ttatacgcaa cttagagggg attcctgcgt catttgcata acaaatccac
      721 tctgttttcc gtttaagatc cggcttgatt cacaacttct ccataaacat ccgcaccgca
      781 ctacggaaat tgtttgtcct taccggactg cttttactcc ttcctcaata cggaaattcc
      841 acccatttgc tgggaggtga tattacctgg acatgcgacg gtacagggaa ttatgttttt
      901 cagctgcgta ttttccgcga ctgttccggt ccatccatca gcattgcagg acaatcgctg
      961 cgggtgtgga accatccaag cattacgcaa atccctctga tctttgtttc gcaaagcgat
     1021 aattctcctg tgtgtacaca agtagcaggc ggacccgtcc agatcacctg cgcatccgga
     1081 aatccgaacg ctgttgagga atacattttc agatcgacac ctgtaaatct aaatgcagtt
     1141 ccaccggcgc aaggatttgc atttacctgg gatgcctttt cccgaccggc ttgtgataat
     1201 ttgcaaaacg cattgattta cggattaaca ctgcgatcgt tcatgtacag caatggcgga
     1261 gcaaacagta atccctgtaa tgattcctca ccgcaattca cttcctcacc gcaatatgca
     1321 ttttgtgcag gagaagcagc caattttggt cagaacgcct acgatcccga tctcgacagt
     1381 gtcgttttca gttggggaac gcccatcgat cagattgcag ccggtacatt caatccccct
     1441 gcatttccca tagctgttcc ctattcaccc aattacagca ttacctctcc actacccgga
     1501 actgcactca atgcgagcaa tgttcccgga caaatcaatc ccgcaacagg cgatatttca
     1561 tttacatcct ttaccaccgg aaaattcaat attgttgtaa aagccgattc gtaccgcaat
     1621 ggtgttttgg tttcggaagt gtaccgcgaa ttcctgatgg tgattgtgaa ttgtggtccg
     1681 aacaccgctc cctcggcaac accggttttt ggtgggggca caagctggtc gggaacattt
     1741 cttgcaggag atttgattca gttcaatctt gttgcaagcg atccggaatt gttgcaggac
     1801 ggatctccgc aatcggtgat catgaccgca acaggattgc agttcggaac taattttaca
     1861 gatcctgcca atggatgtat caatgctccc tgtgcaacat tgagcaatac attaccacaa
     1921 acaggagtgc agggattgag caccgatttc aactggcaaa ccacctgcga tcatataccg
     1981 gccggtgcaa cgcaatcgca atacagtttt acttttttcc tcagcgatga ttattgcagt
     2041 gtacccggcg ccaattcatt tacggcaaat atcacactgc ttgcaccacc atccgttccc
     2101 tcacccgata tccgttgtgc cgatgtagca ccgaatggcg atgtaacatt aagctggaat
     2161 ccatctgctg atccgcaatc catctttgac cgttacgaaa tttacaacag cggaacactc
     2221 attgccacct tgcctgccat tggaacaaac agtttcacgc atgtaggtgc cgatgcacat
     2281 ttgggaatcc gttcctatca catcatcacc tattccgatt gcggaggaat acgcacaacc
     2341 tattccgaca ccatcagcac aatgtttttg caggtgaata atcccgccaa cggaacggca
     2401 caacttgtat ggaatccctt gcgcaatcca tcactaccca gtgcatcggc agtatatgaa
     2461 atctatcgcg aattccctct tggaacttgg acactcatca ataccgttcc tgcaacacag
     2521 catttttatt cggataccat caccgtttgc gatgattctt tgaactaccg aatagaaatt
     2581 tctgatgcac ttggatgtca ttccacttct tccatcgacg gaaaaacatt tcaggatcaa
     2641 atcgcaccct acattcccgt cattaccagc gtaacggtag acacaacaag cggactggcc
     2701 aacatcaatt gggaagtcaa cgcagcaggt gacacccagg gatacatcat acttcaaaat
     2761 cagggaggaa actggatcat catcgatacc gtttatggtg taaacaatac cacttacgaa
     2821 tacgcattat cactggcagg attaaccagc gaatcatacg gtattgctgc attcgacagt
     2881 tgtttttcag gtaatcctcc tactgccaac acctcggcga tgggaacgtt tcacagcagt
     2941 atttacctca ccacttcgct caacatttgt gcgcaaagag ttacgctcaa ctggaatccg
     3001 tatatcaact ggaccggcgg tgttgcgaat tacgaaattt atgcaagcga aaacggtggc
     3061 gcatttcagc tcattggcaa taccaatgca actaattttg tgcatcaaaa tgtaaaccga
     3121 ctttccacct tttgttatct ggttatcgcc tacgaaaacg gaactacgga acgatcactg
     3181 agtaacagag cctgcaggtt cattgcacaa ccatcgcaac ccgcatggaa ctatttacaa
     3241 actgcaacag taagcggcaa caatgtagaa gtgcgttgtt ttaccgatct cagcgcaaca
     3301 gtactcggct atcgcatcga acgcacaacc gatctgagtc tgcccttcga tcctattgga
     3361 acggcagtac caagcggaaa tccggtttcg ttttttgatt attcagcaga tgtcggatca
     3421 agtagttatt actaccgcgc aatcgccatc gacagttgcg gacaggatgc attggtatcg
     3481 aataacggga aaacgatttt gcttactatc gatgcaaatt cacttcgcgg aattgtaacc
     3541 ctgcaatgga atgcctacca gggttgggat ggatttgtaa ccgaatacag gatctaccga
     3601 agcagtaacg gcgtgttcga tcctgctcct attgcaacgg ttttgcccgg aatgttattc
     3661 tacgaagatg atgtttcaat gttcctcgaa agcgacggag agatttgcta ttacgtagaa
     3721 gcggtagaaa gcctgaacag ttttggcatt gcagaaacca gtatttcaaa tcttgcctgc
     3781 gctgttatcg aacccatcgt ttgggtaccg aatgcattta tggttggcgg ggtaaatgag
     3841 atttttattc cggtcatcag ttttgccgat tactccaatt atcaactgcg cattttcgac
     3901 cgctggggac aaatcgtgtt tgaaacaaat gacagagcac agggatggga tggaaaaata
     3961 aacaatcaat tgggtcgcga aggtgtttat gtatgggtat tggaatttgc cgatggctcc
     4021 ggacagctct ttgaaaagaa aggatttgtt actttgttga aagcacccaa atgagaagcg
     4081 tttttctact cctgattttt tcatccctct ttttttctgc ctgccggaaa aacaaggaac
     4141 tcgttgttga gatcaaagga cgcattacaa attcaggaga tcaatcttct gcagcaggtg
     4201 ttaccgtaaa agtgaaattt caggaaatcg gttcgggaag tatttcatcc gccttcaaaa
     4261 caatagctac aactacaaca gatgcttcag gtaattactc ggtgagtttt gaaaaacctg
     4321 cagctgcaga ataccgtttc gaattaagtt cgccaagaca tttcgccact gagatcaatg
     4381 aaagtgccga tgcgttcagc gcaaacgaaa gcaatacccg taatttcgaa ctcgatccgg
     4441 tatccttttt ctcggttcga ttaaaaaata attctcctgt tggtgtgcag gatcagatca
     4501 ttttccaaaa tacctccgag agctattcct gttccatttg ctgcggaaat acaccggtta
     4561 tcaaacaggg tatgactgta gacacctcat tcgtctgcag aagaaaaggc ggacgtttca
     4621 tacgtttttc ctggaccgtt accaaaggag gaatgaatca attctatacg gattcagtga
     4681 tcacaccgaa ttacgacact acttaccgcc tgatcagtta ttaatctatg aaagcattct
     4741 acaccgtatt tcttcttgtt ttcatatcat tctcttttag tgcaagtgct caggacgaaa
     4801 aaaagaaaaa attcctgaaa gatcctgccg accaggccac gatggattct gctgaatttt
     4861 tttaccagga aaaaaattac cacatagcca ttggaaaata caaacgctta tgcgagaatt
     4921 ttcccgatga aaaaatcctt catttccgca ttggagtttg ccagctcaat atgcaggaag
     4981 ccatggagga agcgcttgtc catttcgaaa aactcgatga aaaagcattt gaacgcgagc
     5041 aatcgcttta ctacagagcg agggcattga tgctgaacta ccgttttgaa gaagccattc
     5101 cattgtttga aaaatacaaa gaacaaaaat ggtcgacctt cgaattccgg tttttatcgg
     5161 acatctacat caccaattgc aaaaacggaa ttgaattatt gaaacaaccg gtcgaagcaa
     5221 aaattgaaaa cattggctta ccggtaaaca ccagcgcatc agaatatgtt ccgctgatca
     5281 attcggaagg atctgttctt atttacacct atcgaggccc gcgttctaca ggaggcattc
     5341 aacgaagcac caaccgtaat tccgttgaag caggttccta cgatgaagat attttcattt
     5401 ccaattttga tcccgcaaca ggcgcatggt cagcaccgga gccactcccc ttccctgtaa
     5461 acacgaaagc aaatgatgcc agcgtgtact tgtcggatga tggtcatgaa ttgtacatgt
     5521 tccgttcggt agatggcgat gaaggatcat tgtatgtttc gcatctggaa ggaacagaat
     5581 ggtcgagtcc ggaaaaactt tacggcaaga tcaattctcc ctggtgggaa ggaagtattt
     5641 ccatttctgc cgacggaaga acagcctact tttcaagtga gcgtcccggc ggatttggcg
     5701 gtaaggatat ttatgtagca cagttgcagc ccgacggatc atggggaaat attcgcaatg
     5761 caggaccaaa gatcaacacg cataaagacg acgattcacc atttattcat cccagcggtc
     5821 agttcatcac cttttcatcg caaggacaca attcgatggg cggttatgat atttaccgtt
     5881 gtgatttaac caacgactcc acctggtcgg accccatcaa tatgggtttc cctataaaca
     5941 cacccggcga tgatatcttt tttgtaatca ccggagatgc gcaggcagcc tactacagtt
     6001 caggaaaagc aggaggatac ggcaagcagg atatctacaa aatcattccg gggatattcg
     6061 gaaaacagat ccggatgatt cgcgtgaaag gtgttgtaac cgtcgacgac aaacctgcag
     6121 cttcagaaat ccggatcacc tacgaataca caggagtaac acaaggtatt tatacaggca
     6181 attctgcaag tggaaaatac ctgatcaatc ttccatcaga aaaagaactg gtcatctatt
     6241 ttaaagcaga aggcttcgaa ccacaattgc gaaagatcaa cacgaaagat gtggatgcat
     6301 tccttgaaac taccattgat gtacagtttt atacagacga atacttacga aaacttaaaa
     6361 gcaatggcga cagtttaaaa accattgcca caaacgacgg tacgcttaaa accaacacaa
     6421 gcggaaccga cgatcgcttt tcgaaatttg gaacaacgcg tgtagaagga ctacgtttcc
     6481 atgttcaaat tggagcctac aacatgccgc agaatttcaa ctacagcacg gtaatgaaac
     6541 tgggaaaagt tgaaaaacaa aaactggatg acaacatcac ccgttttgtt attggaacat
     6601 tcgatacctg ggcggaggca aaagagtttt gtcgcttagt gattaaagcc ggtataatcg
     6661 atgcatttgt tacggcagaa taccagggca aacgctacca tattcatgaa ttggtggaaa
     6721 aagggatttt ccaaccctga tcaaaatccg acacaaggca tcattccacc ggaacgcttc
     6781 actttaccgg aagttgtttt cttacgcgcc gaagaatgac gagaaccgcc ggtttgactg
     6841 tagtgcgatc ctttttttcg tttcactgca actgtagctc gcgtttccga agatgaatca
     6901 tcattacctg agttattcaa cgaggaaggt ttgtcccaat gcaatttcgg taaatcaaat
     6961 gttctgctct cgcgggtgga aactgaaatt tcagcgactt cttcgttttc ctgttccgat
     7021 ggtttattgg tttgaaaaac atttacattc tctgcattca cattatccaa tccactattc
     7081 atttccatct gctgcacttc tattacctcc acctcattgg ttttactttg ttctgtattc
     7141 accgcccagt cgttgttggc cacggaagta ttggaggtat tcaccacact tctgttggtc
     7201 tgtacgtttg tctgccgcgc ttgttgttgt gcctgctgtt gcgcttgctg cacctgttgt
     7261 gttggtcgga ccggattgga gcggttggtg ttggagcgat tggtgttggt ggaagtgttg
     7321 gtctgcgaca catggagtgt tgaacgctgc tgtacctgtg ctcttggttc tatcgggttt
     7381 gaaaaattgc tgaattgaat tgtattcgac gaattattga actgagcaaa agccgaggca
     7441 ttcaggaaga acgaacatgc aaacaaaaaa acagggcgca tagaggttat ttttagctct
     7501 gtacaccctg ttttaaataa ggttcaaccc attataaaaa taaaagccct gtactgacaa
     7561 ggcttttaga ataatcaacg aataatcagc tgctgaacga agcgtccccc accgtttctt
     7621 tcaagtacca ccgaataaac accggcaggg aatttttcag catgaataaa gtagcgcgat
     7681 tcacccattg ggaatgaacc cgaatagatt tctgtgactt cttttcctag catatcgtac
     7741 attttgagtg atcccgaaaa tgtgagattc gtttgcacag gaatacaggt aaccgatgaa
     7801 gcaggattcg ggaaaatatt atccatttcc attgcaatca cttcatccac attggaaggc
     7861 gtgcccaata cctgaaaatg gaaatatcct gcaggagccg gcataggacg aacctgtgtt
     7921 ttgccgctga cagctgttcc ttctacataa taataaacat gcgaaccaac agcctgcgca
     7981 ggaatattcg ctacccaatg accattacca gaattcgtca tcgccacagc ttggtatcct
     8041 gtacccggag taagcgacca atacaaatta gcagaagcaa ttcctgattt gtgagtgatg
     8101 taggcatcca ccagataagg gttctgatca tcgtaagtat caggcaaatt ctgatgcgag
     8161 atcagcattg gatcggcagc tccgataagg ttggtgatac aatgaattac accacctgca
     8221 ctgatcagat ttgcgccgga attatcagca tcgatcgtta cgatattgta tccgggcaag
     8281 gcttctgtta gaatacgaat cgcagttgta tcgtattgcg tacggtacac aggaacgata
     8341 gctgttttat ttacgaagac aaaattgggg taattgcgat acgcaccatt tccaaaagga
     8401 gcacccggcc agtttccgct ggtagaggga accatcggaa cacgaatcac tttatacggt
     8461 gttccgaata cggaattaaa gttactcagc acat
//
LOCUS       DAOMYG010000077         8455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2796_length_8455_cov_9.697024, whole genome shotgun sequence.
ACCESSION   DAOMYG010000077 DAOMYG010000000
VERSION     DAOMYG010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8455
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2796_length_8455_cov_9.697024"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PK610_03805"
     CDS             complement(<1..757)
                     /locus_tag="PK610_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35082.1"
                     /translation="MKLLRYTVFLLLFLPFVGKAQHEAVFNTKIITTYNVRSIPGLNT
                     AFSEFSPLIAGERLVFVSDRDFDYLNWGENRWKKRKFMNVFSAGFQLVADSAKTQSPV
                     IYSATLVGQNHSGPIAISPDGATIVFTQVGKKRHTVNRPQLYLTRKVDGKWSNPQLLS
                     FCDADHSYGHPAFSMDGNVLFFSSDREGTKGGKDIFYSRLENDAWGMPENAGVMINTS
                     GDEVFPFVHENVLYFSSNCSGGNGELDIYKSYFR"
     gene            complement(769..1716)
                     /locus_tag="PK610_03810"
     CDS             complement(769..1716)
                     /locus_tag="PK610_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRJ35083.1"
                     /translation="MKRTLHLFSLSLSALFFLVAFSGSTLVNAQQDALYSQYMFNPFM
                     INPAYAGSRETMSGTLILREQWRGIEGAPSTQTFSMHSPLPKENMALGINIFNDGIGP
                     TRNTGMLLTYAYHLKLGKAKLSFGLRGGMFNSRLDVSKLNYFDPNDRFSANGATRALV
                     PTFDFGTYLYTKSFFAGLSVNHLLENKINYPNLPADAYMVMKRHYLFSTGAAIQIGEN
                     TVFKPTALVKYTEGAPINIDINTSFLFSKALWLGAGYRSSKSVVIIAEYNITDYLRAG
                     YSYDIVFNKLKSFTTGTHEIMLGFDLNIKKTQSISPRYL"
     gene            complement(1726..6846)
                     /locus_tag="PK610_03815"
     CDS             complement(1726..6846)
                     /locus_tag="PK610_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ35084.1"
                     /translation="MAKLFGSLRHILILFPVFISISVKSADRYVTTTANAGPGSLRDQ
                     IGISAAGDRVIMGISGTVALTSPITVTTSVEVIGAFPVHNTINFSGVGINNCAFNVTG
                     GQLSIRGFLFHNPSLNLNVGAIQVANTASLLVNDCVFEQGRANTGGAILTNGPTIINS
                     SCFTQNSSVSDGGAIYVNGAAATCTLTNCTFSANNSNANGGAIRAINGPALVIRHCTF
                     KGNTATNLGKDLSVAIAGTTVTLHNNIFDQGVLGSLSNSGAANFVSQGGNIFTFANPG
                     FVTTSTNDAFSVLPALLNLGLLVTDGYGMRFHRINSNSSPATDNGTAGTLPVKDCRRA
                     PRIMQGNATMNPDAGAVEFSQYTVVNSNATGAGSFANAVSQVNANATPAPYYIDFIIP
                     APTPILLGATLTINNPVIIDGYIQSGSQIGGPGNVLNSLTSLTPIIILDGSGATTNGI
                     ILGNTAINSFISGLNIHGFVNNGIVIGGASGCKIHGNMVHSNGTHGIEIQGAAGSNTI
                     GDTSLYSINMISNNTARGIYINNSPSNTIKNNLVGTDQIGTTIFGTQAVGIEISGVAA
                     TGNLIGGVSHFFQGNILSGNTAHQVFINASANANTVSGNKFGLTYNGQNSLSSAAANG
                     VTISASNNTIGGTTLGRNNIICGQGGAGIFINGGTSNSIFKNNIGYSPIAAVSIANTT
                     GILISGTSTGNTIGASNGRNYISGNSGNGVRISSGAGGNTLTNNFIGISELNNAIGNA
                     GYGIFIETGSNGNAIGTALGGNYISSNGNSGIGISSSRNNSLLNNTIGLDSSRTLMRS
                     NTVHGIEINNGANTQVGGNFVCFNLGKGIDIVSDSVNLFNNLIGTDLTNSINQGNGGH
                     GISIAGHRNRVGNGLTADNTIAFNNGAGVAVISGTENGISKNIILNNSGLGIDLGAVG
                     VTPNDLNDADAGANTLINFADNFLSTQCGTGTTIAGEFYGIAGNTYRIDFYQVPGAGQ
                     DPSGHGEGNLWLGSSNITVTTTGYTFFSYFHATLLTTGTIVTATVSKFVAAGGYFETS
                     EFSQNTSVVAGITAVNHTSTNVTCAGSANGGASATPVGGIAPFTYSWFTSTNTQIAGQ
                     FLSTIGSLAPGSYYGVAMDASGCPDTTTVFTITEPLPITATASGSNPLCNGSCDGTIT
                     VTATGGTGIIQYSNDGGTTFQAPSVFNALCAGSYVNLVVTDQNNCNTPVPSVTLVNPP
                     VITVVASAAVDVTCPSLCDGSITLTATGGTGALNYSIDNGTTFNATTTFSGLCTGVYN
                     TQMQDANGCLTAIVPITVGTQTVLQANAGPDLSVCLGAPAILDGSGSTNALTYSWYAS
                     GNATLLGSTAVFTTSPPAGTTYVLTITNGTCTSNDTVVVNILPIPDPNVVYPFATSDT
                     LYICIGGSTLTPTITTPGGTFTATGGININAATGVADFSATSFGIFNIFYTTATCNQR
                     DTIVALILPDPVAPVSLQYDYTICSGDPIPTFNVDPTGVLGLMTWYNTSALTIPISVG
                     FAFTPPNLPATYNDYFAAVNYTNCIGPSTMFTVSVIDNTAITATSPVSICTGETTTLD
                     LVVNTGTLTSIVWSPGGTLNDSTIVQPTASPFQNTIYSYQALIGSCTLNGTIDVQIST
                     DPNCVFQEVYNAFSPDGDGVNELWILPAAGFFPNNTVTIFNRWGDAIREFTNYDNVSV
                     VWDGTNKGGQQLPSGTYFYVVRYHDADRQYSGWIQLTR"
     gene            complement(6974..7711)
                     /locus_tag="PK610_03820"
     CDS             complement(6974..7711)
                     /locus_tag="PK610_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35085.1"
                     /translation="MKRLILILLIFPPFGILKAQERMLTLGIQYKPIFASRFFGAGEI
                     ITKDDALEASITPTWGHSYGMIIRRGFTKMISYEGGISYVKRRYAINCTDVDSGFSSP
                     SDFSLVSYEIPNQMLIYIQLGERLYMNTAFGISLIAFASDVQSFATDRRFYHFSKLTT
                     NFLSAALIANVGFEYRTKKSGFFYFGASFHNPVKDITRSYIEYSKDNFSTYRADFGLR
                     GTYLTLDFRYFFHEAPIREQKNRTKKK"
     gene            complement(7708..>8455)
                     /locus_tag="PK610_03825"
     CDS             complement(7708..>8455)
                     /locus_tag="PK610_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRJ35086.1"
                     /translation="SALLIISTDSLNHFAISWDNVHPFNQSIPLDLFDTLFLLRLNST
                     TFGYHRPVSDQTIRKYGIRVGSPFGWRDGKNHNGVDIELDQWDSIPCAFDGVVRMAKN
                     YSGYGKVVIVRHYNGLETLYAHMARISVKSGQTVKAGQLLGLGGSTGNSTGSHLHFEI
                     RYKNIPLNPSNVIDFREGKLLSDTLMMRKMRTSFVCTPVGVHFHTVERGDYPHKIATR
                     YGLSVTQLCEMNNISSRTRLRVGQRLRVSS"
BASE COUNT         2361 a         1711 c         1872 g         2511 t
ORIGIN      
        1 ccctgaaata ggatttataa atatcgagtt cgccatttcc tccgctgcag ttgctggaga
       61 agtaaagcac attttcatgg acaaacggaa atacttcatc gccgcttgta ttgatcatca
      121 ctcctgcatt ttcaggcata ccccatgcat cattttccaa ccgtgaatag aaaatatctt
      181 tcccgccttt tgttccttct ctgtcgctgg agaaaaacag cacattccca tccatactga
      241 atgcaggatg tccgtatgaa tgatcagcgt cacaaaatga cagtaattgc gggttcgacc
      301 atttaccgtc gacttttctt gtaaggtaaa gttgaggacg gttaaccgta tgccgctttt
      361 ttcctacttg ggtgaaaact attgtagcgc catccggtga aattgcaata ggcccggaat
      421 gattttgacc aaccagcgtt gcggaataaa taacaggact ttgagttttt gcactatctg
      481 caacgagttg aaatccggcg ctgaaaacat tcatgaactt tcttttcttc cagcgattct
      541 caccccagtt gaggtaatcg aaatcgcggt cggaaacaaa taccagtcgt tcgcctgcaa
      601 tgaggggtga aaattcacta aatgcagtat tgagaccggg tatggatcga acgttgtaag
      661 tggttatgat tttggtattg aataccgctt catgttgtgc ctttccgaca aacggtagga
      721 acaacaatag aaaaacggta tagcgtaata acttcatggt gtggtgtcct acaggtatcg
      781 tggtgaaatg gattgcgttt ttttgatgtt caaatcgaaa ccgagcatga tttcatgtgt
      841 tccggttgtg aaacttttta gtttgttaaa tacgatgtcg taagagtacc cggcacgtaa
      901 ataatcggtg atgttgtatt ctgcaataat cactacagat ttactgcttc tgtatccggc
      961 tcccagccat aatgctttgc taaacaaaaa tgaggtgttg atgtcgatgt ttatcggtgc
     1021 tccttctgtg tattttacaa gtgcggtggg tttaaaaaca gtattctcac caatttgtat
     1081 tgccgcaccg gtagaaaaaa gataatgtcg tttcattacc atgtatgcat ctgctggaag
     1141 atttggataa ttgatcttgt tctccaacaa atgattgacg gatagtcctg caaaaaatga
     1201 ttttgtatag aggtaagttc cgaaatcaaa agttggaacc aatgcgcggg ttgctccatt
     1261 agcactgaaa cgatcattcg gatcaaagta atttagtttc gaaacatcta gtctggaatt
     1321 aaacattcca ccacgtaatc caaatgagag ttttgccttg ccaagtttaa gatgataggc
     1381 gtaggtaagc agcatcccag tgttacgtgt tggaccaatt ccatcattaa aaatgtttat
     1441 tcccaaagcc atgttttctt tgggtaacgg actatgcatg ctgaatgttt gagttgaagg
     1501 cgcaccttca attcctctcc attgttctct taagatcaat gtaccgctca ttgtttccct
     1561 gcttcctgcg taggcagggt ttatcataaa cggattaaac atgtattggc tgtaaagcgc
     1621 atcctgttgt gcattaacca gtgttgaacc ggaaaatgct accaggaaaa acagggcaga
     1681 aaggctaagc gaaaataagt gaagcgttct tttcatgttc ctttcttaac gtgtaagttg
     1741 aatccatcct gaatactgtc ggtcggcatc gtgatacctc acaacataaa aataagttcc
     1801 cgatggaagt tgttgcccgc ctttattggt tccatcccat acaactgaaa cgttatcgta
     1861 attcgtgaat tctctgattg catcacccca gcggttgaaa atggtaactg tgttgtttgg
     1921 gaagaatcct gcagcaggta gtatccacaa ttcatttacg ccatctccat ccggactgaa
     1981 tgcattgtac acttcctgaa aaacacaatt cgggtcggtt gagatctgaa cgtcgatagt
     2041 tccgtttaat gtacaggaac caattaatgc ctgataggag taaatagtat tttgaaatgg
     2101 agaagctgtt ggttgtacaa tggtcgaatc atttagcgtt cctccaggtg accaaacaat
     2161 acttgttaat gttcctgtat tcactacaag atctaatgtt gtagtttcac ctgtgcagat
     2221 tgaaacaggt gaagtagcag tgatggctgt gttgtcaata acagaaacag tgaacatggt
     2281 tgaaggtcca atgcagttgg tgtagttcac agctgcaaaa tagtcgttgt aagttgccgg
     2341 tagattgggc ggggtaaagg caaaacctac actaatagga atagtaagtg cagaagtatt
     2401 gtaccaggtc attaatccaa gtactcctgt tggatctacg ttgaatgttg gaatcgggtc
     2461 gccagagcaa atggtgtaat cgtattgaag cgatacaggt gctacaggat ccggaagaat
     2521 aagtgcaaca attgtatcgc gctggttaca ggttgcagtt gtatagaaaa tattaaaaat
     2581 gccgaagctt gttgctgaaa aatcagctac tcccgttgca gcgttaatgt taattcctcc
     2641 ggtagcagta aatgttcctc caggagttgt aatagtgggt gtaagtgtag atccaccaat
     2701 acagatgtac aaggtatcac tggttgcaaa aggatataca acatttggat ccggaattgg
     2761 taatatgttt acaacaacag tatcgttgct ggtacaggtt ccattagtga ttgttaaaac
     2821 ataggtggtt cctgctgggg gagaggtagt gaatactgct gtgcttccca ataaagtagc
     2881 atttccactt gcataccaag aatacgtaag ggcatttgtg ctcccggatc cgtcaagtat
     2941 tgccggcgcg cccaaacaga cgctaaggtc aggaccggca tttgcctgca acacagtttg
     3001 cgttccaact gttattggaa ctattgcggt gagacatccg tttgcgtctt gcatctgtgt
     3061 attgtaaacc ccggtacaaa gaccggaaaa tgttgttgtg gcattaaaag ttgtaccgtt
     3121 atcgatgctg taatttagcg cacctgttcc acctgtagca gtaagtgtaa ttgatccgtc
     3181 gcacaaagaa gggcaggtaa catcaacagc agctgatgca acaactgtta ttacaggagg
     3241 attaaccaat gtaactgagg gaaccggcgt gttacagttg ttctgatctg ttacaacaag
     3301 atttacataa gatccggcgc ataatgcgtt aaatacagat ggtgcctgaa aagttgttcc
     3361 tccgtcgttg gagtattgta ttattccggt tccaccggtt gcagtaactg taatagtacc
     3421 atcgcatgat ccattacata atggattact tcctgatgct gttgctgtaa tgggaagtgg
     3481 ttcggtaatc gtgaaaacag tggttgtatc cggacatccg cttgcgtcca ttgcaacacc
     3541 ataatagctt cctggtgcaa gtgatccaat ggtgcttaaa aactgtcctg caatttgtgt
     3601 atttgtgctt gtgaaccaag aataggtaaa cggagcaatt ccaccaaccg gggtggcaga
     3661 tgcgcctcca tttgctgatc ctgcgcaggt gacattcgtg ctggtgtggt ttactgcagt
     3721 aattccggca acaacagaag tattttgact gaattcagaa gtttcaaaat atccgcctgc
     3781 ggcaacaaat ttacttacgg ttgcggttac aattgttccg gtagttaata gtgttgcgtg
     3841 gaaataggaa aagaaagtat aaccggttgt tgtaacagtt atgtttgatg atcccagcca
     3901 taaattccct tctccatgtc cggaaggatc ctgaccggca ccgggtactt gataaaaatc
     3961 gattcggtaa gtgtttcctg ctattccata aaactcgccg gcaatcgttg tccctgttcc
     4021 acattgcgtg gaaaggaaat tgtcggcgaa attgatcaat gtattagcac ctgcatcggc
     4081 atcgttcaaa tcatttggtg taacaccaac tgctccaaga tcgattccca atccggaatt
     4141 attcagaatg atgtttttac taattccatt ttctgtgcct gaaattaccg caacacccgc
     4201 accgttattg aaggcaattg tattgtcagc tgttaatcca tttccaaccc tgtttctgtg
     4261 tcctgcaatc gaaataccat gaccgccatt gccttgattg atgctgttag taagatcagt
     4321 tccaattaaa ttgttgaaca gatttacgct gtcagataca atatcaattc cttttccaag
     4381 gttaaaacaa acaaaattgc ctccgacctg agtgtttgct ccgttgttta tttctatccc
     4441 gtgaacggta ttgcttcgca tgagcgtgcg tgaactgtca agtccaatgg tgttatttag
     4501 caatgaattg tttcgggagg aggaaatgcc aattcctgag tttccatttg aactgatata
     4561 gtttccaccc aatgctgttc caattgcatt accattggat cctgtttcaa taaaaattcc
     4621 gtaccctgca tttccaatcg cattattaag ctcactaatt cctataaaat tatttgtcag
     4681 cgtatttccg ccggcaccgg aagagatcct tactccgttt ccgctgtttc cggaaatata
     4741 gtttctgcca ttgcttgcgc cgatcgtatt cccagtggaa gtgccgctga tgagtatacc
     4801 agttgtattg gcaattgaaa ccgcggcaat aggtgagtac ccaatattgt ttttgaaaat
     4861 ggaatttgat gtacctccgt taatgaagat accagctcct ccctggccac agatgatgtt
     4921 attccgtcct aaagttgtac cgccaatcgt attattggat gcggaaatgg tgacgccatt
     4981 tgctgcagca gaacttaatg aattttgtcc gttataagtt aggccaaatt tgtttcctga
     5041 aacagtattt gcgtttgcgg atgcattgat aaaaacctga tgagcggtat ttcctgaaag
     5101 aatgtttccc tggaaaaaat gggacacacc tccaataagg tttcctgttg ctgctacacc
     5161 cgaaatttca ataccaactg cctgtgttcc gaaaattgtt gttccgattt gatcggttcc
     5221 aacaagatta tttttaattg tatttgaagg agagttgtta atgtaaatgc cacgtgctgt
     5281 attattggag atcatattga tactgtacaa agacgtatca ccgatcgtat tactacctgc
     5341 ggctccttga atttctattc catgggtccc attgctatgt accatgttcc catgaatttt
     5401 acaaccggac gctccgccaa ttacaattcc gttgtttaca aatccatgta tattaagtcc
     5461 gctgataaaa ctattgattg cagtattccc taagattatt ccgttggtag ttgctcctga
     5521 gccatccaaa ataattattg gtgttaaact ggtaagagag ttaaggacat ttccaggacc
     5581 accgatttgg cttccggatt gaatataacc atcaataatt accgggttgt ttatagtgag
     5641 cgtcgcacca agtaaaatag gtgttggagc cggaatgatg aagtcgatgt agtagggtgc
     5701 cggtgtagca ttagcattta cctgcgaaac cgcgtttgca aacgatcctg ccccggttgc
     5761 attcgaattc acaacagtgt attgcgaaaa ctcaacagct ccggcatccg gattcattgt
     5821 ggcatttccc tgcataatgc gtggtgccct tctgcaatct tttactggga gtgttcctgc
     5881 tgttccatta tctgttgccg gtgaggagtt gctgttgatg cggtgaaaac gcattccgta
     5941 tccatctgta accagcagac caagatttaa caatgctggt aaaaccgaaa aggcatcatt
     6001 ggtggatgta gttacaaatc ccggatttgc aaaagtgaaa atatttccac cttgactaac
     6061 aaaatttgct gctccggaat tggaaagcga acctaaaaca ccttgatcaa aaatgttgtt
     6121 gtgaagtgta actgttgtac cagcaatggc aaccgataag tctttaccaa gatttgttgc
     6181 cgtatttcct ttaaaggtgc agtgacggat aaccagtgcc ggaccattaa tcgctctgat
     6241 tgctcctcca tttgcattgg agttgtttgc tgaaaatgta caattggtta aagtgcatgt
     6301 tgcggcggct ccattcacat atatcgctcc tccgtctgat acgcttgagt tttgtgtaaa
     6361 gcagctggag ttaatgatgg ttggtccgtt ggttaaaatt gcacctcctg tatttgcccg
     6421 tccttgttca aacacacaat cattcaccaa tagactggct gtattggcga cttggatggc
     6481 tccaacattt agatttagag aagggttgtg aaacaaaaaa ccacgaatgc ttagctgtcc
     6541 tccggtaacg ttaaatgcgc agttatttat ccctaccccg gagaaattga tggtattgtg
     6601 aacggggaag gcaccaataa cttctaccga tgtggttacg gtaattgggg aagtcaaagc
     6661 gacggttccg ctaataccca tgattacccg gtctcctgct gctgaaattc ctatctggtc
     6721 cctcagtgaa cccggtcctg cgttggctgt agtggtaacg tagcgatcgg cactcttaac
     6781 cgatatggaa ataaataccg gaaataaaat aagaatgtgc cttaaagacc caaaaagttt
     6841 cgccatggtt tgtgtttggt gtgtgtcaca aaaacagacg gcaaaaatag gttaaagttg
     6901 cgccttgtaa gggtaaaggg gtgagaaaaa atggttaaag tttcgaccaa tcaaggaaaa
     6961 tacaggatgt accttatttc ttttttgtgc gattcttttg ttcccgaatt ggggcttcat
     7021 ggaaaaaata gcgaaaatct aacgttaaat aggttccgcg cagaccgaaa tctgctctgt
     7081 aagtgctgaa attatccttc gaatattcga tatacgacct tgtgatatct ttaacagggt
     7141 tgtgaaatga ggctccaaaa tagaaaaatc cggatttttt ggttcggtat tcgaagccaa
     7201 cattggcaat tagtgcggca cttagaaaat tagtagtcag tttcgaaaaa tgataaaaac
     7261 gtcggtctgt tgcgaaggat tgtacgtcgc tggcaaaggc aatgagtgag attccaaagg
     7321 ctgtattcat atacaaacgc tcacctaact gaatgtaaat tagcatttga ttagggattt
     7381 cgtaggaaac caaactgaaa tcggaaggag aggaaaagcc gctatctaca tctgtacaat
     7441 tgatggcata gcgccttttt acatagctga ttccgccttc gtaggaaatc atttttgtga
     7501 atcctctgcg aatgatcatt ccgtaactat gtccccaggt gggagtaata ctggcttcaa
     7561 gggcatcatc ttttgtaatg atttctcccg caccgaaaaa gcgacttgca aaaatgggtt
     7621 tgtattgaat tcctaatgtt agcatccgct cttgcgcttt aagaatgcca aaagggggaa
     7681 aaataaggag gatcagaatg agtcgtttca tgagcttaca cgtaaacgtt gaccaactct
     7741 taaacgggtt ctcgaagaaa tattgttcat ttcacaaagc tgagtaactg ataatccata
     7801 ccgtgttgcg attttatggg gataatctcc gcgttctact gtgtgaaagt gtactccaac
     7861 cggtgtgcaa acaaatgaag ttctcatttt acgcatcatc aaagtgtcgc tgagtaactt
     7921 cccttcacgg aaatcaatca cgttggaagg attaagaggt atgtttttgt atcggatctc
     7981 aaaatgaaga tgacttcctg ttgaattccc cgtgcttcca ccaagaccca gtaattgacc
     8041 ggctttaaca gtctgaccgc tttttacgct tatcctcgcc atgtgggcat aaagcgtttc
     8101 taacccgttg tagtgacgta cgataactac tttgccatag ccggaataat tttttgccat
     8161 tcttactact ccatcgaacg cacaggggat actgtcccat tgatcaagtt cgatgtcgac
     8221 tccattatgg tttttaccat ctctccatcc aaagggtgaa ccaacacgga tgccgtattt
     8281 gcgaatggtc tggtcgctga ccggtcgatg atatccgaat gtggtggaat ttaaacgaag
     8341 aagaaaaagg gtgtcgaaca gatccaatgg aatgctctga ttgaagggat ggacattgtc
     8401 ccaggaaatt gcaaaatgat ttaaagaatc tgtagaaatg attaaaagtg ctgat
//
LOCUS       DAOMYG010000078         8391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2829_length_8391_cov_7.945897, whole genome shotgun sequence.
ACCESSION   DAOMYG010000078 DAOMYG010000000
VERSION     DAOMYG010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2829_length_8391_cov_7.945897"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1821)
                     /locus_tag="PK610_03830"
     CDS             complement(<1..1821)
                     /locus_tag="PK610_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRJ35087.1"
                     /translation="MKIEQLLEDRVLVIDGAMGTMIQRYQLSEEDFRGDRFKGHSHPL
                     KGNNDLLAITRPDVLLAIHKQYLEAGADILETNTFSGTWIAQADYKLESAVYDINFLS
                     AQIAKKAVEEFYRENPEAEKTTPRFVAGAMGPTNRTASLSPDVNDPGFRAVSFDELVQ
                     AYAEQTRALIEGGVDLLLVETVFDTLNAKAALYAIQLVFEEKKMELPVMVSGTITDAS
                     GRTLSGQTVEAFLNSISHVKLLSVGLNCALGAKDMRPYLEELSAKAPFFVSSYPNAGL
                     PNQFGEYDESPHEMACTIHDFLESGFVNIVGGCCGTTPDHIAHIAEHARKSKPRKRPQ
                     IEPYLRLSGLESLTLRPESNFMNVGERTNVTGSRKFAKLIIEGNYDEALSIAREQVEG
                     GAQVIDINMDEGMLDAVEAMTRFLNLIAAEPDIARVPVMIDSSKFHVIEAGLKCTQGK
                     GIVNSISLKEGESAFIEHAKTVKRYGAAVIVMAFDEKGQADTLPRRIEICERAYGILV
                     DKVNFPPQDIIFDPNIFPVATGMEEHRRNAIDFFEATKWIKTNLPHAKISGGVSNVSF
                     SFRGNDVVREAIHSAFLYHGIKAGMDMGIVNAGQLVVYDEI"
     regulatory      complement(1903..2034)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2070..2333)
                     /locus_tag="PK610_03835"
     CDS             complement(2070..2333)
                     /locus_tag="PK610_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010137118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35088.1"
                     /translation="MRIGLVFLFLFLGALLFNSDVEAQCAMCKAVAETGSQGQDQSSI
                     GEQLNIGIIFLMAIPYLFILIIPMILFRRHIKGFFRELRDVHK"
     gene            complement(2345..2872)
                     /locus_tag="PK610_03840"
     CDS             complement(2345..2872)
                     /locus_tag="PK610_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HRJ35089.1"
                     /translation="MSDTIDLEKKYKPLIVSVSIAVPLVVALLFRVRIEGWDTAGLPP
                     VYATINALTAVLLIAAVASIKAGKRSLHERLIKLCILLSAAFLVMYVLRHMTSDPTPY
                     GGTGINRYFYYFVLVTHIALSVIIIPLVLFTYVKAWSGKFDQHKKWARITFPVWLYVA
                     VTGVVVYLMIFPYYQ"
     gene            complement(2865..3494)
                     /locus_tag="PK610_03845"
     CDS             complement(2865..3494)
                     /locus_tag="PK610_03845"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRJ35090.1"
                     /translation="MMKNSFLYMLLVFLFACTASEKQKNGSGLPFLGHHDIDPVNSDT
                     VYHIVPPFAFENQDGAIITNDNVKEKIHVVNFFFTSCPSICPKMMSQLKRLQKLSDGM
                     DIVILSYSVDPKRDSVPRLKRYADENGFSTVNWHLMTGDADEIYELGMTGYNLSAMED
                     ESADGGFLHSEMVVLVDKKGRLRGMYEGTQTKAMDQLLNDIKKLQKEDE"
     gene            complement(3481..4128)
                     /locus_tag="PK610_03850"
     CDS             complement(3481..4128)
                     /locus_tag="PK610_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35091.1"
                     /translation="MERLNKKIIFFLIMMFVPFTFFYLFSRGSQHFKRLPFFGTYSAD
                     SVPFSYPDFSFTSSTGVISKENLKGKIIIMTAMGSDCPDNCDFLVKPFRLDVYNGILK
                     KNKEFKDVIILSEMVDTIHTRLEDMPEKLKVDAQQWNFFVSENKLFWDVELNGNSILR
                     TDDKDRPGKMMWTRSILLIDPDFRLRGWYDGVHGVELRRLIDELRLLKKELNDEE"
     gene            complement(4132..4506)
                     /locus_tag="PK610_03855"
     CDS             complement(4132..4506)
                     /locus_tag="PK610_03855"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HRJ35092.1"
                     /translation="MERDDIIEYSLNTHHSEEDGRKIRKKIWQVTALLTLITAVEIFV
                     GVKFPRYAVSDTTWLMIKFFYTILTIIKAGYIVLVFMHLGDERKNLRWAILAPYGLFI
                     LYLIFICLTEGTFVNFSWTNFL"
     gene            complement(4536..5306)
                     /locus_tag="PK610_03860"
     CDS             complement(4536..5306)
                     /locus_tag="PK610_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRJ35093.1"
                     /translation="MSAHASHPVDASSLWGGKESPFSVSYGKLMMWFFLVSDALTFGG
                     LLTAYGFARHAYTGEWPASDHVFTHLPFTHAHAPLIYVALMTFILIVSSVTMVLAVEA
                     GHRMDKKGVIKWLGLTVVGGAIFVGSQAWEWYTFLAGPDGDFWNFGPAGDNSTLWYNS
                     LATLRDADGVAILNEHGHEQVGADGPQQYAMFFFFITGFHGFHVFSGVIINIVVLIQV
                     IQGIYHKRGHYEMVEKAGLYWHFVDLVWVFVFTFFYLL"
     gene            complement(5331..6089)
                     /locus_tag="PK610_03865"
     CDS             complement(5331..6089)
                     /locus_tag="PK610_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRJ35094.1"
                     /translation="MNTEATVAEDDRYSPEKLKARGAKGMLYIGIASIVMLFSAFCSA
                     FIVSKGGNFWVNLILPQAFWISTAIILLSSLTVNMALNYVKRNNQKMTSLFLSLTLVL
                     GILFSIYQWLGWGQLIERGNYFVGNIMDPDESGFFVKGEYGKDFTLTFDNKELDYKEG
                     RFFYPDGKELSAVQYENLKRQRNTASSYIYIFTALHLLHLIGGLIYLIFVTLASYRGK
                     YTAENHLRISLIAIYWHFLDLLWILLFVFLQFIH"
     gene            complement(6086..6997)
                     /gene="cyoE"
                     /locus_tag="PK610_03870"
     CDS             complement(6086..6997)
                     /gene="cyoE"
                     /locus_tag="PK610_03870"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRJ35095.1"
                     /translation="MRKFIPTLIAQQTYTTPDRLTFMLRLREYAVLTKLRLSFLVVVS
                     AITGYLMADTFAGVDFSLLIFGGFLVTGSSNGFNQVIEREYDKLMTRTQNRPLVKGSI
                     SITEALIVSSLFGLVGLVLLYLINPLSAILGFLALFSYVAIYTPMKRISPWAVFVGAF
                     PGAIPPMLGFVAATGHFGLLPGLLFFVQFMWQFPHFWAIAWVLDDDYRKAGYRLLPSK
                     FGRNHSSSFQILLYTCAMIIASASPYVMELTGTLSLIVCMVAGLFFYRYALNLHHSQE
                     NSDARKVMFASFVYLPIVQFIYVFDKL"
     gene            complement(7050..>8391)
                     /locus_tag="PK610_03875"
     CDS             complement(7050..>8391)
                     /locus_tag="PK610_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35096.1"
                     /translation="QWKPNDSDSTFPTHGFYVQPHFRAILQSDIANDTSYFGRGLGAG
                     ITYTNRNKFAANFSFVTDNSPYPEYLRPSISAGNAIPGMGYAHPTNEGYAFTAFSGYA
                     SWSPHKIFNFQAGSGKHFFGDGYRSLLISDNAYNYPYLRMSATFWQIKYVVLYSMMND
                     LGASQGDPKQFRRKYATTHFLNWNISRRFSLGIFETVVWQAKDSLLNRQFDVNYLNPF
                     VFFRPVEYAQGSADNVLMGLNAKLKITDHYQIYGQFILDEFLLNEMRADSGWWANKYG
                     YQLGAKSFSPFGLKNLYLQTEYNFVRPFTYSHRSVVQNWGHMGSPLAHPYGANFREWI
                     NIGRFTYKRFQFEETFLWTIYGLDTGSVSWGGDPFKSYSQRARNYHNHVGQGQRNEVL
                     FHQFRISWIVSPTWNARVFIGHQYRKQQTDLGIHETHMLVFGLSSWLWNQYIDR"
BASE COUNT         2652 a         1879 c         1555 g         2305 t
ORIGIN      
        1 tatttcatca tatacaacca actggcctgc atttacaata cccatatcca taccggcttt
       61 tatcccatga tacaaaaacg cagaatgtat ggcttccctt acaacatcat tacctctgaa
      121 tgaaaacgaa acattactaa ctccaccact tatttttgca tgcggcaaat ttgttttgat
      181 ccattttgtt gcttcgaaaa aatcaatcgc atttcgacgg tgctcctcca ttcctgtagc
      241 aaccgggaaa atatttggat cgaaaatgat atcctgagga ggaaagttga ctttgtcaac
      301 caatattccg taggctcgct cacaaatttc tattcgtcgt ggcaatgtgt cagcctgtcc
      361 cttttcatca aatgccatta caataaccgc cgctccataa cgtttaaccg ttttcgcgtg
      421 ttcgatgaat gcactttcac cttcttttaa tgaaattgaa ttcacaattc ctttcccttg
      481 tgtgcattta agcccggctt ctattacgtg gaatttcgat gagtcaatca tcactggaac
      541 ccgtgcaata tcaggttccg cagcaatcaa attcagaaag cgcgtcatcg cctcaacagc
      601 atcgagcatt ccttcgtcca tattgatatc gattacctgc gctccaccct ctacctgttc
      661 tcttgcaata gacaacgcct cgtcataatt gccttcaata atcagttttg caaattttcg
      721 cgaccccgtt acattggtgc gctcaccgac attcatgaaa ttactttccg gacgaagagt
      781 cagcgattcc aatccactca agcgcaaata cggttctatt tgtggccgtt ttcgtggctt
      841 tgacttgcgt gcatgttctg cgatgtgtgc aatatgatcg ggtgtagtgc cacaacatcc
      901 tccaacaata tttacaaaac cactctccag aaaatcatga atggtacacg ccatttcatg
      961 aggactctca tcgtattcgc caaactgatt gggtaaaccg gcattaggat aggaactcac
     1021 aaaaaacggt gcttttgcag aaagttcttc gaggtagggg cgcatatcct ttgcaccaag
     1081 tgcacaattc aatcccacac ttaataattt aacatgcgaa atcgaattta aaaatgcctc
     1141 gacggtttga ccggataatg ttcttccgct ggcatctgta attgtgccgg aaaccattac
     1201 aggaagctcc attttcttct cttcaaaaac aagttgaata gcatataacg ctgcctttgc
     1261 attaagcgtg tcaaataccg tttcgaccaa tagaagatct actccacctt ctataagcgc
     1321 tcttgtttgc tcagcataag cctgaaccaa ttcgtcaaaa ctaactgcac gaaaaccggg
     1381 atcatttaca tccggtgaaa gtgatgctgt tcgattggtt ggtcccattg ctcctgcaac
     1441 aaaacgaggt gtggtcttct ccgcttccgg attctctctg taaaactcct caactgcctt
     1501 tttagcaatt tgggcagata aaaaattgat atcgtaaact gccgattcca gtttgtagtc
     1561 cgcctgggcg atccatgtgc ctgaaaaagt gtttgtttcc agtatatccg caccggcttc
     1621 cagatactgt ttatgaatgg ccaataaaac atcaggccgg gttattgcca gcaagtcgtt
     1681 attgcccttt agaggatgac tatgcccttt aaaacgatcg ccacggaaat cttcttcaga
     1741 caattgataa cgttgaatca ttgtccccat tgcgccgtca ataacaagca cacggtcttc
     1801 aagtaattgt tcaatcttca ttttcaaaca tcattaaata aaaaatccct ccggtagccg
     1861 gcgggatcac atatgagaat tcatctgaag aatcaaccat ccgacttatc tttcctcgtt
     1921 ttcacgaggc aggaattggc accttctgtg caaaaaacaa cttttacagc tgcctaataa
     1981 tcagggttgc caaggcatca aagggctctg tccctccgcc tttctggata agtgtaccga
     2041 aagaactggt gtaaagataa ggaatagttt tatttatgga catcacgaag ctcccgaaaa
     2101 aagcctttaa tgtgacgacg aaacaggatc attggaatga tcaggatgaa caaataagga
     2161 atagccatca aaaagatgat accgatattt agctgctccc caattgagga ttggtcttga
     2221 ccttgagaac ctgtttcagc aacagccttg cacatggcgc attgcgcttc cacatcggaa
     2281 ttaaacaata aagctcctaa aaagagaaac aaaaaaacta atccaatacg cataaatcaa
     2341 aaaattattg atagtacggg aaaatcatca aataaaccac tactccggta acagctacat
     2401 ataaccaaac aggaaaagta attcttgccc acttcttatg ctgatcaaat tttcctgacc
     2461 atgcttttac gtaggtaaag agtacaagcg gaataataat aacagaaagc gcaatatggg
     2521 ttaccaaaac aaaataataa aaatacctgt ttatccctgt tcctccatat ggcgttggat
     2581 cacttgtcat gtggcgtaaa acatacatta ctaaaaaagc agccgataac agaatgcaaa
     2641 gtttaatcaa tcgctcatgc aaggatcgtt tccctgcttt tattgatgca acagctgcaa
     2701 ttaaaagaac tgctgttagt gcgtttattg ttgcataaac cggaggtaaa cctgcagtat
     2761 cccaaccctc aattcgcacc cgaaacaata gcgcaacaac taatggtact gcaattgaaa
     2821 ccgatactat tagcggctta tatttttttt caagatcaat tgtatcactc atcttccttc
     2881 tgcagtttct ttatatcgtt caacaactga tccattgcct ttgtttgcgt gccttcgtac
     2941 atcccgcgca atcgtccttt tttgtcaacc agtaccacca tttctgaatg caaaaaccct
     3001 ccatcagccg actcatcttc cattgcgctc aaattgtaac ctgtcattcc gagttcgtaa
     3061 atctcgtctg catcacctgt cattaagtgc cagttgacag ttgaaaatcc attctcatca
     3121 gcgtatcgct tcaatcgggg cacgctgtct ctttttggat caaccgaata agaaagaatg
     3181 acgatatcca ttccgtctga cagtttttgt aatcgcttaa gttgagacat catttttgga
     3241 caaatcgatg ggcatgatgt aaagaagaaa tttacaacat ggattttttc cttgacattg
     3301 tcattagtga ttattgctcc atcctgattt tcgaatgcaa aaggcggtac tatgtgataa
     3361 acagtgtctg agttaaccgg atcaatatcg tggtgcccca gaaaaggaag cccactgccg
     3421 ttcttttgct tttcacttgc tgtacatgca aataaaaaaa ccaaaagcat atataaaaaa
     3481 ctattcttca tcattcaatt cctttttcaa caggcgcaat tcgtcaatca atcttctcag
     3541 ctcaacgccg tgaacaccat cgtaccaccc tcttaatcta aaatccggat cgataagcaa
     3601 aatagacctt gtccacatca ttttgcctgg ccggtctttg tcgtctgttc tcaatattga
     3661 attgccattt agctccacat cccaaaacaa tttgttttcc gaaacaaaaa agttccattg
     3721 ctgtgcatcc acttttaatt tttcaggcat atcttcgagc cttgtatgta tagtgtcaac
     3781 catctctgaa agaataatta catccttgaa ctctttgttc tttttcagaa tgccgttgta
     3841 cacatccaga cgaaatggtt taacaaggaa atcgcaatta tcaggacaat cacttcccat
     3901 tgccgtcata ataatgatct ttcctttcag attctctttg gatatgacac ctgttgaact
     3961 tgtaaatgaa aaatcaggat agctaaaagg aacactatcg gcagaatagg tgccaaaaaa
     4021 aggcagtcgc ttaaaatgtt gactgcctct tgaaaaaaga taaaaaaatg taaagggcac
     4081 gaacatcatg atcaaaaaga agatgatctt tttgttcagc ctttccattt tttacaggaa
     4141 gttggtccag gagaaattca caaatgttcc ctctgtaaga caaataaaaa tgaggtaaag
     4201 tataaaaagt ccgtagggag caaggatggc ccatcgtaag tttttacgct catcaccaag
     4261 gtgcataaac accaaaacga tgtacccggc cttaatgatg gttagaatgg tgtaaaagaa
     4321 cttaatcatc aaccatgttg tatcgcttac agcatagcgt gggaatttaa ctcctacaaa
     4381 aatctctact gcagtgataa gtgtaagcaa tgctgtaacc tgccagatct ttttacggat
     4441 tttacgtccg tcttcttctg aatgatgggt gttgagtgaa tactcaataa tgtcgtctct
     4501 ttccatgact ggttcttatt aaaatgagtg aggaatcaaa ggagatagaa gaaggtaaat
     4561 acgaataccc aaacaagatc tacaaagtgc cagtaaagac ctgccttctc taccatttcg
     4621 taatgaccac gcttatggta aattccttga attacctgga tgagcaccac gatattgata
     4681 attacaccgg aaaaaacgtg aaatccgtgg aatccggtaa taaagaagaa gaacattgca
     4741 tattgctgcg ggccatctgc cccaacttgt tcgtgaccat gttcattaag aattgcaaca
     4801 ccatctgcat cacgcaatgt agcaagagag ttataccaaa gtgtggaatt atcacctgca
     4861 ggaccaaagt tccagaaatc accgtcagga ccggcaagga atgtatacca ctcccaagct
     4921 tgtgagccaa cgaaaattgc ccctccaaca actgtaagtc ctaaccactt gataacacct
     4981 tttttatcca ttctgtgccc tgcttctacc gcaagaacca tagttaccga ggagacgatc
     5041 agaataaacg tcatcaacgc aacataaata agtggtgcat gagcatgtgt aaacggcaag
     5101 tgggtaaaca catggtctga tgctggccat tctcctgtat aggcatgacg ggcaaagccg
     5161 taagccgtaa ggagaccacc aaaagttagg gcatccgata caaggaaaaa ccacatcatc
     5221 agtttaccat agctgacgga gaatggagac tcttttcctc cccaaagaga agatgcgtca
     5281 acaggatgtg aagcgtgtgc agacataggt tgtacgagtt tggcgcaagt ttaatgaata
     5341 aattgtaaaa agacgaacaa aagaatccaa agcagatcca aaaaatgcca gtaaatagca
     5401 atcagactaa ttctcaaatg attctctgcg gtgtatttac cgcgatagga tgccagagtg
     5461 acaaaaatca ggtaaatgag tccccctatc aggtgcaata aatgcagagc ggtgaaaata
     5521 taaatgtatg aacttgcggt attccgctgt cttttaaggt tttcatactg aacagccgag
     5581 agttcttttc catcaggata gaaaaaacgt ccttccttgt aatccaactc tttattatca
     5641 aaggttaatg tgaaatcctt accatactcc cctttcacaa aaaaaccgct ctcatcagga
     5701 tccattatat tccccacaaa ataattcccg cgctcaatta actgccccca tccaagccat
     5761 tgatatatag aaaacaaaat ccctaacact aatgttagtg acagaaaaag tgaagtcatt
     5821 ttctgattat tacgcttcac atagtttaat gccatattta cggtgaggga actaagcaaa
     5881 attattgcgg tagatatcca aaaggcctgt ggaagaatga ggtttaccca aaagtttcct
     5941 cctttcgaaa caataaaagc gctgcagaag gcggagaaca acatcacaat actggcgatt
     6001 ccaatgtaaa gcattccttt tgccccgcgt gcttttaact tttcgggcga atacctatcg
     6061 tcctcagcaa ctgttgcttc cgtattcata gcttatcaaa aacgtaaatg aattgaacaa
     6121 tgggtaaata aacaaaagag gcaaacatca cttttcgcgc atctgagttt tcctgagaat
     6181 ggtgcagatt tagcgcgtaa cggtaaaaaa acaatcctgc caccatgcaa acgatcaatg
     6241 aaagtgttcc tgtaagctcc attacataag gcgatgcgct tgcaattatc attgcgcagg
     6301 tatacagcaa aatctgaaaa ctagaggaat gattccttcc gaatttggat ggtaaaaggc
     6361 gatagccggc ctttcggtaa tcgtcatcaa gcacccaggc gattgcccaa aaatgcggga
     6421 attgccacat aaattgaaca aaaaataata gtccgggtag caaaccaaaa tgaccggttg
     6481 ccgccacaaa gccaagcatt ggtggaatag ctcccggaaa tgcgccaaca aaaacagccc
     6541 agggtgaaat acgtttcatg ggtgtgtaaa tggcaacata ggagaacaat gccaaaaacc
     6601 caagtattgc gcttaaggga tttatcaggt acaataatac caggccgacc aatccaaata
     6661 atgatgatac aataagggct tcggtaatgc taattgaacc ttttaccaat ggacgattct
     6721 gggttcgtgt cattaactta tcatattcac gctcgataac ctgattaaat ccatttgagg
     6781 aaccggttac caaaaatcca ccaaagatca acagcgagaa gtctacaccc gcaaatgtat
     6841 cggccatcag ataacctgta attgcagaaa caacgaccaa aaaagaaaga cgaagttttg
     6901 tcaaaaccgc atactcacgt aacctgagca taaaggttag ccgatccggt gtggtatatg
     6961 tctgctgcgc aatcaaggtt ggaataaact tgcgcaaagg taggattatt gaataaagtg
     7021 aatcgaaaaa aataaagatt ggaatgattc tatcgatcaa tgtattggtt ccataaccag
     7081 gaacttagac caaaaaccag catatgagtt tcgtgtattc ccagatccgt ttgctgttta
     7141 cggtattggt gtccaataaa aacacgggca ttccaggtag gtgatacaat ccaggaaatt
     7201 cgaaactgat gaaacaaaac ctcattacgc tgtccctgcc caacgtgatt gtgataattt
     7261 cttgctcgtt gtgaatacga tttaaacgga tcgccacccc aactcacaga acctgtatct
     7321 aaaccataaa tggtccacag gaatgtttct tcaaactgaa accgcttata ggtaaatcgc
     7381 ccaatgttga tccattccct gaagtttgca ccatacggat gtgccaatgg agaacccata
     7441 tgtccccaat tctgcactac cgatcgatgc gaataggtaa atggtcttac aaaattgtat
     7501 tcagtctgca agtatagatt cttcaaacca aacggagaaa atgatttagc ccctaactgg
     7561 tatccatatt tatttgccca ccagccgcta tcagcacgca tttcattcaa taaaaactca
     7621 tccaaaataa actgaccata aatctggtaa tgatctgtta tttttaattt ggcattcaag
     7681 cccatcaaca cattatccgc agatccttga gcgtattcca ccggcctgaa aaaaacaaaa
     7741 ggattcagat aattcacatc aaattgcctg ttgagcaagc tatcctttgc ctgccatact
     7801 acggtttcaa atattcccaa tgaaaatcgt cgggaaatat tccaattaag aaaatgcgtg
     7861 gttgcatatt ttcgtctgaa ttgttttgga tcgccctgtg aagctcccaa atcattcatc
     7921 atggaataca gtaccacgta ttttatttgc caaaaagtgg ccgacatccg cagataaggg
     7981 tagttgtagg cattgtcgct tatcaacaaa gaacggtaac catccccaaa aaaatgtttt
     8041 cctgatcctg cctggaaatt gaaaatttta tgcggcgacc acgatgcata gcctgaaaaa
     8101 gcagtgaagg catatccttc atttgttgga tgagcgtatc ccattccggg aattgcattg
     8161 cctgctgaaa tagatggcct taaatactca ggatagggtg aattatcagt aacaaaagaa
     8221 aagtttgcag caaatttatt tcggtttgta taggttattc cggctcctaa acctcttcca
     8281 aaataggatg tgtcattggc aatatcgctt tgaagtattg ccctgaaatg aggctgaaca
     8341 taaaaaccat gtgtgggaaa agttgaatcc gaatcatttg gtttccattg t
//
LOCUS       DAOMYG010000079         8388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2830_length_8388_cov_10.396256, whole genome shotgun sequence.
ACCESSION   DAOMYG010000079 DAOMYG010000000
VERSION     DAOMYG010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8388
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2830_length_8388_cov_10.396256"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1239
                     /locus_tag="PK610_03880"
     CDS             115..1239
                     /locus_tag="PK610_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRJ35097.1"
                     /translation="MDLSSRLSGQSRQELISFLRERVKELSCLYEFQKIFQKTDSDLI
                     QLLEEARRVIPAGMQHSEFAESFIILDAITLGDITKPDSKSFIEALIKIDGRNRGKII
                     VYYNSPDHSLNFLEEEKLLLNKLADEISWLVERTEQKAIQQQLEEQMTRSDKLVLVGE
                     IAAGFAHEINTPLGSILGFSELLMNMDLPEEAKRDLQRIHHAALHSREIVKRMMMLSG
                     DYPLQIVPNNIGECINWSVEMLGPQAAAKGVQISVTLDKEIDVFSFDRIQMIQVLLNL
                     ISNAVNALHEGGNVYVETRKEHDNIVLIVRDNGPGILPELREKIFTPFFTTGKIGQSA
                     GLGLAVVQSIIRNHGGEITMRSHTGKGTEFCITLPTALNK"
     gene            1236..2570
                     /locus_tag="PK610_03885"
     CDS             1236..2570
                     /locus_tag="PK610_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008236960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRJ35098.1"
                     /translation="MKQHRKTILVVDDSPDLLVLLRKQLEARDHIVFTAPGVMDAVTI
                     LESEMLDLLITDMRMPGIGGLQLVKYARIKRPDLPILVITGFPSVESAVEAVKFGATE
                     YLVKPFTANELYQSIENISGQKQHVESSDKENTETKEEDTFGIIGTHPLIVSLREHIR
                     KASGNNATVLIQGESGTGKELVARAIHYEGKGAGKPFVAINCGAIPESLLESELFGFV
                     KGAFTGAQESRIGLLIAADKGTVFLDEINATSPAFQAKLLRVLQDREVRMIGSTKAQQ
                     LDIRIIAAGNSLLEDMVERGTFRMDLFYRLNVVRINVPPLRDRTTDIPLLVSHFMEKF
                     KREYAKHHMEISPIAIEGLKRYDWPGNVRELENTIQRLVIECDRSIEYDDLPVVKSRK
                     TVTVSVQQDKFLTLKEMEEHYIRQVLEHTGNNKTEAARILGIDRKTLREKLK"
     gene            3024..4301
                     /locus_tag="PK610_03890"
     CDS             3024..4301
                     /locus_tag="PK610_03890"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRJ35099.1"
                     /translation="MSTTIEKKTGMYENVMRQFEKAADTMNLDPGIRSILSTTQNEIV
                     VHFPAKMDNGRIEMFTGYRVQHNNILGPYKGGLRYHPTVDIDAARALASWMTWKTALA
                     GLPYGGGKGGVQIDPSKYSLSELERITRRFAFALGENIGPDMDIPAPDVNTNAQMMAW
                     IADTYTSTRPATQRSNSMHVVTGKPVGSGGLEGRDRATGFGVVISIREWAKSSGMNLR
                     GMKYIVQGFGNVGQWTAHFLKKEGAILIAVQDASGTLYNENGIDPDLLLEYVRNNGGN
                     IAGFTGAETIDASAFFQTECDIVIPAALGNQITAANAGSINAKVIAEGANGPLDVDGE
                     TILLKRGIHIIPDILCNGGGVTGSYFEWLQNKNGSIWTIEDVLAGLEKMMVIAYGKVQ
                     HTADKYKTDHRNAAYIVALERIAQAYKERGVFP"
     gene            4298..5428
                     /locus_tag="PK610_03895"
     CDS             4298..5428
                     /locus_tag="PK610_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HRJ35100.1"
                     /translation="MSNQISKIGLIKEPVTETRVALLPEDVRKLVETSPGVSIVFEAN
                     LAVHIGISDSRYLEAGAIRMESSSEIIDYSDVIVCIQPVYDLRLSRKDKVFVGWFNCL
                     NQLNHLSIYKEGRFTILSLDLLPRTTIAQRMDSLSSMASLNGYKAVIKGTDQLGLALP
                     MMTTAAGTIKPGNTLVVGAGVAGLQAIATAKRLGARVSAFDVRAAAAEEARSLGARFL
                     EVKGEINKSSVGGYAVEQSAQMLIEQQELLHEAAISSVLIICTAQIPGRRAPVLITEK
                     SIYGMQPGSVIVDLAAETGGNTELTEKDKTIEVNGVTIIGNSNLHREIPYTASRLLSS
                     NFYHFIHHLLAKGMEDDLILQTAAVKNGRIIHPKIIQLIEES"
     gene            5428..5751
                     /locus_tag="PK610_03900"
     CDS             5428..5751
                     /locus_tag="PK610_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HRJ35101.1"
                     /translation="MEQLNQFFFEHTETIYFVVLCVLLGVEVISGVPAMLHTPLMSGA
                     NAIHGVVLIGALLVTLQLDSNDYAGLIIGALAVFLGTLNVAGGFFVTGRMLEMFKPKP
                     DKKRK"
     gene            5751..7142
                     /locus_tag="PK610_03905"
     CDS             5751..7142
                     /locus_tag="PK610_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRJ35102.1"
                     /translation="MIHSIVQMGYLISGIGFILGLKMMSSPQKAVRGNFIAASGMILA
                     VVLTIIYCLYVQPVIPYVSLLTVAVAIALGTIVGYLISYKVQMTAMPQMVSLFNAMGG
                     GSAMLIGWMELQHGVQLAGTWIILSAGAMIGAVSFSGSGIAYLKLSGKKVEAANYGVR
                     TLGFFLPLLLAGGMLIGHSWFSSQAIAITVLIGIALVYGVIFVLPIGGADMPVVISLL
                     NSFTGMAAGLAGIAYQNKVMIIGGILVGAAGLLLTLLMCKAMNRSIMNVLLGAFKKEN
                     KKSKGDVGNLGVIQETDASEVALNLAYAGKVAIIPGYGLAVAQAQHACHQLEKMLEEH
                     GVETIYVIHPVAGRMPGHMNVLLAEADVSYEKLIEMDEVNDQMDAFDVVIVIGANDVV
                     NPAAEQNSSSKIYGMPIIRAHRAKSVVVIKRSMNAGYAGIENELFHLPNCKMLFADAK
                     KGLNDIATAMKKF"
     gene            7142..7477
                     /locus_tag="PK610_03910"
     CDS             7142..7477
                     /locus_tag="PK610_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35103.1"
                     /translation="MRFFSGKEVQEFRQLDVVLVLHSARIAQGNASNMVLRVEGIREN
                     RKEFLETIELKNFKCDVSGNIRFYCNDPSQPLIIDLRNSIRVSISVYSEEYFDLNLHV
                     ITYKNLPGS"
     gene            complement(7482..>8388)
                     /locus_tag="PK610_03915"
     CDS             complement(7482..>8388)
                     /locus_tag="PK610_03915"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRJ35104.1"
                     /translation="YLNFSARTKNMSRSLLGHLAILIANLIYGINYTVAKEVMGTYIQ
                     PFGFILLRVAGTIALIWTTALLFVNEKVAKEDHRRLLLCGLTGVGINQMLFFWGLSLT
                     SPINSSIIMVTTPLLVTIIAGILLKEGISTKRGAGLFIGLTGAVILLVLKPVDNSVSG
                     DLFGDFITFLNACSYAVYLVLVKPLMKKYKPVTVMKWVFLYGLIPVLPFGTEQFFAIE
                     WSTFTTNAIWAVVFVVAGTTFLAYLLNTYGLVTLSPGVVSAYIYLQPALAALVAVMLG
                     KDQLDTVKIGSTALIFIGVYLVNKN"
BASE COUNT         2515 a         1532 c         1976 g         2365 t
ORIGIN      
        1 ggatgatttc agaatttgat gtggtttatt gcttatgggt tcataatggt aaatccttac
       61 ctttgagttg ttcagtgttt gggtgtttca tatgaattat caaatagtta gaagatggat
      121 ctttcttccc gtttaagtgg gcagtcccga caggaactca tctccttttt gagggaaaga
      181 gtcaaagaat tgtcgtgtct gtatgaattc cagaaaattt ttcaaaagac ggattcggat
      241 cttatccaac ttttggagga ggcaagacgt gttattcctg ccggaatgca gcactcagag
      301 ttcgctgaaa gttttataat tcttgatgca attacacttg gcgacatcac taaaccggat
      361 agtaaatcct ttattgaagc attgatcaag attgatggaa gaaacagggg aaaaatcata
      421 gtttattata attctccgga tcattcctta aattttcttg aagaggaaaa attgctttta
      481 aataaactgg ctgatgaaat ttcatggttg gtggaacgaa cggaacaaaa ggcgattcaa
      541 caacagcttg aagaacaaat gacacgctct gacaagctgg tgttggttgg tgaaattgca
      601 gcgggttttg cgcacgagat aaacacaccg ttaggtagta ttcttggttt ctccgagttg
      661 ctcatgaata tggatttacc cgaagaagcc aaaagagatc ttcagagaat tcatcatgca
      721 gcacttcact cccgtgagat tgtaaagcga atgatgatgc tttccggaga ttatcctttg
      781 caaatcgtgc ctaataatat cggtgagtgc attaactggt cggttgaaat gttgggaccg
      841 caggcagctg caaaaggagt tcaaatcagt gtaacactcg ataaggagat tgatgtgttt
      901 tcattcgaca ggattcaaat gattcaggta ttgcttaacc ttatttccaa tgcggtaaac
      961 gcactgcatg aaggagggaa tgtatatgtt gaaacacgga aagagcatga taacatagtt
     1021 ttgattgtca gggataatgg tccgggtatt ttgcctgaat taagagagaa aatattcaca
     1081 ccctttttta ccacaggaaa aatcggacaa tcagcaggtc tgggattagc tgttgtacaa
     1141 agcatcatca gaaaccacgg cggagaaatc acgatgagaa gtcatacagg aaaagggaca
     1201 gaattttgta ttacattacc tacagcatta aacaaatgaa acagcataga aaaacaattc
     1261 ttgtagtcga cgattctcct gatttgctgg ttttattacg caagcaactt gaggcaagag
     1321 atcatatagt tttcactgcg cccggagtga tggatgcggt gactatttta gaaagcgaga
     1381 tgttggattt gctgattact gacatgcgga tgccgggtat tggaggattg cagctggtaa
     1441 aatatgcaag aataaagcgt cctgatttgc ccatcctcgt gattacagga tttccgagtg
     1501 tagaatccgc cgttgaagca gtgaagttcg gagccacaga gtatctggtg aagcctttca
     1561 ctgcaaatga actttaccaa tcgatcgaaa atatttcggg acaaaaacag catgtcgaaa
     1621 gcagcgacaa agaaaacaca gagacgaaag aagaagacac attcggaatt attggcacgc
     1681 atccgctaat cgtttcccta cgggaacaca tccgtaaggc atctggaaat aatgcaacgg
     1741 ttttgattca gggggaaagc ggtactggta aagaattggt tgcaagagct attcactacg
     1801 aaggaaaagg cgcaggaaaa ccgtttgttg caatcaactg tggagccata cctgaatctt
     1861 tacttgaaag tgagttgttt ggatttgtaa agggagcatt tacaggcgca caggaatccc
     1921 gcattggttt gctcatagct gcagataagg gtaccgtttt tttagatgag atcaacgcaa
     1981 catctcctgc ctttcaggca aaactgttac gtgtattgca ggatcgtgaa gtaagaatga
     2041 tcggctcaac aaaagcccaa caactcgata tacggataat cgcagcagga aatagtttat
     2101 tggaggacat ggttgaaaga ggaacgttcc gcatggatct attttaccga ttaaatgttg
     2161 ttcggataaa tgttccaccc cttagagatc ggactactga tattccgtta ttggtttcgc
     2221 attttatgga gaagttcaaa cgtgaatatg caaaacatca catggaaatt tcccccatcg
     2281 ccattgaagg actaaagcgt tatgattggc ctggaaatgt cagagaactt gaaaatacta
     2341 ttcagcgtct ggttattgaa tgtgatcgat ctattgagta cgacgattta ccggttgtga
     2401 aaagccgaaa aacagttacc gtatcggttc aacaggataa atttctcacc ttaaaggaaa
     2461 tggaagaaca ctatattcgt caggttcttg agcataccgg aaataataaa acagaagcag
     2521 cacgtatttt agggatcgat cgtaaaacac ttcgggagaa acttaaataa cccggacatt
     2581 atacctgctt ttttcgtgca tttaaatagg ggagatattc cccgatggag tgttttgatc
     2641 cggggaataa taccccaatt cattttttag gattttagtt tgtttgatta tcaggatgtt
     2701 gatagtcgga tttccacatg gcatactttt cctataggga gttatagaat tcaatatcta
     2761 tcaggatgag tacaagttta tgtataagtt gtttgtggca aagcggatgt tcgctcaaag
     2821 cagatgttcg cgcaccaatt cttcaatgcg aagaattctc tttagctgaa ccttttccac
     2881 caaaaattaa agagcatagt ctgcatatat tttcagcacc aaagcattcc ggtatttgct
     2941 caacatgcga caaaaaccac aattgtgcat tacggaaaga aaatgaaata aaaatccatt
     3001 gtgaacacta tcaataaaac atcatgagca cgacaattga aaaaaagact ggcatgtacg
     3061 aaaacgtcat gcgacaattt gaaaaggcag cagataccat gaaccttgat cccggaatta
     3121 ggtcaattct ctctacaacg cagaatgaga ttgttgtcca tttccctgct aaaatggaca
     3181 atggtcgaat tgaaatgttt accggttacc gggtgcagca caataatatt cttggcccct
     3241 acaaaggagg acttcgatat catcccacag tggatattga tgcagcacgc gcgcttgctt
     3301 cgtggatgac ttggaaaaca gcattagccg gacttcctta tggtggaggc aaaggaggag
     3361 tacaaattga tccttcaaaa tattcattga gcgaacttga gcgcattacc cgtcgttttg
     3421 catttgcgct gggtgagaat atcggtcctg acatggatat tcccgctccg gatgtaaata
     3481 ccaacgctca aatgatggca tggattgccg atacctatac ttcgacgcgg cccgcaactc
     3541 agcgaagcaa cagtatgcat gttgtaaccg gcaaaccggt agggtctggc ggactcgaag
     3601 gtcgcgaccg ggcaacagga tttggtgtgg ttatttccat tagagaatgg gcaaaaagca
     3661 gcggaatgaa tttgcgcggt atgaaataca ttgtgcaggg atttggaaat gttggtcagt
     3721 ggacggctca ctttttgaaa aaggaaggag caatacttat tgcagtacag gatgcatcag
     3781 gaacattgta caacgaaaac ggaatcgatc ctgatctgtt acttgagtat gtgcgcaaca
     3841 atggcggtaa cattgctgga tttactggag cagagacaat tgatgcatca gcgttttttc
     3901 aaactgaatg tgatattgta attccggcgg ccctgggcaa ccagattact gctgctaatg
     3961 ccggtagtat caatgcaaaa gtgattgcag aaggagcaaa tggtccgctg gatgtagatg
     4021 gtgaaaccat tttgttgaaa aggggaatac atattattcc cgatatactc tgtaacggtg
     4081 gaggagtaac aggaagttac tttgagtggt tgcagaataa aaatggtagt atctggacaa
     4141 ttgaagacgt gcttgcaggt cttgagaaaa tgatggtaat cgcatatggc aaagttcagc
     4201 atacagcaga taaatacaaa accgatcatc gcaacgcagc ctatatcgta gccttggaaa
     4261 gaattgccca agcctataag gagagaggag tatttccatg agcaatcaga tttcgaaaat
     4321 cggcctgatt aaagagccgg ttacggaaac aagggttgct ttattaccgg aagatgtcag
     4381 aaagttagtg gaaaccagtc ccggtgtttc cattgttttc gaagcaaatc tggctgttca
     4441 cattggtatt tccgactcaa ggtatcttga agcaggagca atacggatgg aaagttcatc
     4501 ggagatcatt gattattctg atgtaatagt atgtattcag ccggtatatg atttacgtct
     4561 ttcgcggaag gataaagtat ttgtcggatg gtttaattgt ttgaatcagc tcaatcactt
     4621 gtcgatttac aaagagggca ggtttaccat tttaagtttg gatctgcttc cgcgtacaac
     4681 aattgcgcag cggatggatt cgctttcttc tatggcttcg ctaaacggct acaaagcggt
     4741 gatcaaggga acggatcagc ttggacttgc cttgccgatg atgaccactg cagccggaac
     4801 aataaagccc ggaaataccc ttgttgttgg cgcaggcgtt gcgggattgc aggctatagc
     4861 taccgctaaa cgtttgggcg cacgtgttag tgcctttgat gtacgcgctg cagctgcaga
     4921 agaagcacgt agtctgggtg cgcgattttt ggaagtgaaa ggagaaatca ataaaagttc
     4981 ggttggagga tatgccgttg agcaaagcgc tcaaatgcta atagagcaac aagaactttt
     5041 gcatgaggcg gctatttcct ctgtgcttat tatttgcacc gctcagattc ctggtagaag
     5101 agctccggtt ttgattacag aaaaatcaat ttatggaatg caacccggta gcgtaattgt
     5161 tgatctcgct gctgaaaccg gtggaaatac ggaactaaca gaaaaggata aaaccattga
     5221 agtaaacggt gttacgataa ttggaaattc aaatttgcac cgggaaattc catataccgc
     5281 atcccgactt ttatcttcca atttttatca ttttatacac catcttcttg ccaaaggaat
     5341 ggaggatgat ctgatccttc aaacagctgc tgttaaaaac ggccggataa tccaccccaa
     5401 aatcattcaa ctaattgaag aatcgtaatg gagcaattga atcagttttt ttttgagcat
     5461 acagagacca tctattttgt tgtgctctgt gttttattgg gcgtagaagt aatttcagga
     5521 gtgccggcca tgttacatac tccgctgatg tcgggagcaa atgcaattca tggtgttgtt
     5581 ctcattggag cgctcttggt cacacttcaa ctggatagca atgattatgc tggattgatc
     5641 ataggagcat tggcggtttt tcttggaaca ctgaatgttg ccggtggttt ttttgtaacc
     5701 ggacgaatgc ttgaaatgtt taaaccgaaa ccagataaaa aacgtaaata atgatacatt
     5761 ctattgtaca gatggggtat ttgatctcgg gaatagggtt tattctggga ttaaaaatga
     5821 tgagttcacc acaaaaagca gtgcgcggta attttattgc agcatcagga atgattctcg
     5881 ctgttgtgct aactattatt tattgtcttt atgttcaacc ggtcattccc tatgtttctt
     5941 tactaaccgt tgcagttgca attgcgctgg gtaccattgt tggttatctg atatcctaca
     6001 aagtgcaaat gacagccatg cctcaaatgg tttctttatt taatgccatg ggaggaggct
     6061 cggcaatgtt gattggttgg atggaactgc aacatggtgt acagttagcc ggtacatgga
     6121 taattctaag tgcaggtgcg atgattggag ccgtttcttt ttcgggaagt ggtattgctt
     6181 atttaaaact atcagggaaa aaagtggaag ccgctaatta tggtgtgcgc acacttggat
     6241 tctttcttcc cttgttgttg gccggtggaa tgcttatagg tcattcatgg ttttcttcgc
     6301 aggcaatagc aattaccgtt ttaattggaa ttgcacttgt ttacggggtc atttttgtac
     6361 ttcctattgg aggcgccgat atgccggtgg tgatttctct cctgaattct ttcacgggaa
     6421 tggcggcagg attggcagga attgcctatc aaaataaggt gatgattatt ggaggaattc
     6481 ttgttggtgc agcaggatta cttctaacac ttttaatgtg taaagcaatg aaccgctcaa
     6541 ttatgaatgt gttattagga gcattcaaaa aagaaaataa aaaatctaaa ggcgatgttg
     6601 gtaacctggg cgtcattcaa gagacagacg catcggaagt tgcattgaat cttgcttatg
     6661 ccggtaaagt agccattata ccgggatatg ggttagcagt tgcccaggcc caacacgctt
     6721 gtcatcaact tgaaaagatg ctggaagaac acggagtgga aacgatttat gtgatacatc
     6781 ctgttgccgg aagaatgccc ggtcatatga atgttttgct ggccgaagct gatgtttcct
     6841 atgaaaaact tattgaaatg gatgaggtaa acgatcagat ggatgcattt gatgtggtta
     6901 ttgttattgg cgcaaacgat gttgttaatc ctgctgctga gcaaaactca tccagtaaga
     6961 tctatggtat gccaataatc agagcgcacc gggcaaaaag tgttgttgta attaaacgta
     7021 gtatgaatgc aggatatgct ggcatagaga atgaattgtt tcacttgcca aactgtaaaa
     7081 tgttgtttgc agatgccaaa aagggattga acgatattgc tactgcaatg aagaaattct
     7141 aatgcgtttt ttttcaggta aggaagtaca ggaattcagg caactggatg tagttctggt
     7201 cttgcacagc gcacgtatcg ctcaaggcaa tgcgtcgaac atggtattaa gagtggaggg
     7261 cattcgtgaa aacagaaagg aatttttaga aacgatcgaa ctgaaaaact ttaaatgtga
     7321 tgtatcaggg aatatccgtt tttattgcaa tgacccttcc caaccactta ttattgacct
     7381 gagaaattca atacgcgttt caatttcggt gtattcagag gaatatttcg atttaaatct
     7441 tcatgttatt acctataaaa acttaccggg aagctaagcg ctcagttttt attcaccaga
     7501 tacactccaa taaaaatcaa tgctgtggag ccgattttta cggtgtcaag ctgatctttt
     7561 ccgagcataa cggctaccaa tgctgccagc gcaggttgca ggtaaatata ggcgcttaca
     7621 acacccggac tcagggtcac aagtccataa gtgtttaaca aataagctaa gaaggttgta
     7681 cctgctacta caaatacaac ggcccaaatt gcattggtgg tgaaggtaga ccattcaatg
     7741 gcaaaaaact gctctgttcc aaatggtaga acaggaatta aaccgtatag aaagacccat
     7801 ttcattaccg tgacgggttt gtattttttc attagtggtt ttaccagcac cagataaaca
     7861 gcgtacgaac aggcattaag aaatgtaatg aaatcgccaa agagatcgcc gctaaccgaa
     7921 ttgtcgaccg gctttagcac aagaagaata acggctcctg ttaatccgat gaataatcct
     7981 gcaccacgtt tcgtgcttat gccttcttta agaagtattc ctgcgataat ggtcaccagc
     8041 aagggtgtgg tcaccataat gatggaggag ttgatgggag aagtcagtga taatccccag
     8101 aaaaacaaca tttggttgat gccaacacct gttagtccgc ataacaataa ccggcggtga
     8161 tcttcttttg ccactttttc attcacaaaa agaagagcgg ttgtccaaat cagcgcaatg
     8221 gttccggcaa cacgcagaag aatgaagccg aagggttgaa tgtatgtccc cattacctct
     8281 tttgccacgg tataattgat accgtaaatg agattggcaa taaggatagc cagatgtcca
     8341 agtaggctgc gtgacatgtt ttttgttctg gcgctaaaat taaggtaa
//
LOCUS       DAOMYG010000080         8343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2847_length_8343_cov_10.476351, whole genome shotgun sequence.
ACCESSION   DAOMYG010000080 DAOMYG010000000
VERSION     DAOMYG010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2847_length_8343_cov_10.476351"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2308)
                     /locus_tag="PK610_03920"
     CDS             complement(<1..2308)
                     /locus_tag="PK610_03920"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ35105.1"
                     /translation="MVRYFSLLMFFTLLSLNGMAQPVQTIRGKVVDKETQMPLPGANV
                     RILGDSTGKSGASTDADGKFRIEGVPVGKRSVKISFIGYMDKVLDIEVSSAKEIVLSI
                     ELEESATTIAEVEVVARQRGEVNNDMALTSTRSFDVAETERYAGSRGDPARMASNFAG
                     VQGADDSRNDIVVRGNSPLGVLYKFEGFDLPNPNHFAIAGSAGGPVSVLNNKVLANSD
                     FFTSAFPAEYGNSTSAVFDLRMRPGNNQKHEFSGQLGFLGTELAAEGPINKKTGSSYM
                     LVYRYSTLSLFQSLGISLGTDAVPRYQDLSFKFNFPTKKGGNLSFFGIGGKSLIDILI
                     SNQTAPKADFYGEDDRDQYFRTQMGMTGISYTKTINEKTFMRVGIAQAHEQNRAEHFY
                     ILRHVDAASNLFVVDSIYQILNYRFRVSRTTGMVSFNTKLNKQHVLRYGINLNMIRFN
                     MWDSAVSFIDDQWKHRWQNDEYGVLGQAYLQWKWRPNEKLAMTAGLHSQFFSISQSIS
                     PIEPRIGMRYTINNKSAVFFGTGLHSQTQPYYTYFYRYQNPDGSLKAPHNKGMGFTKS
                     FHSVLGYDYAFNPTMRLKAETYYQYLYEIPVEQNPSSFSLTNMGSGFSRFFPDTLVNK
                     GTARNYGVEVTLEKFFNKSFFFLVTGSVFDAKYRGSDDTLRNTDYNTKYAANFLFGKE
                     FSINKKSTIGLGAKFTTAGGRWHGYVDSLASATNNELIFRKDGNNSMQFRPYYRFDLK
                     INYKINAKKITHEIALDLVNVLGVQNILN"
     gene            2440..3123
                     /locus_tag="PK610_03925"
     CDS             2440..3123
                     /locus_tag="PK610_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35106.1"
                     /translation="MKRLSNLIFYPALLIGVVYALYLWFSGQPIGSGEFVYTGDPNRW
                     TDVFFKVIFGLVLGAYLVNVVDRYSGLLFFLRAEKNEKILHNAVLGSQALRWSGMTFG
                     IGIPLALLCGGFLMIADTHSYLAGVVVVVSALEKLIYFVVCRSKKLFRAALTPNALVI
                     HNGGLQIFSFGGLRSIDSKYDELYFVYNENDIRSVFRYLTEEKEREAFFHHLELMAKD
                     KNIPVSIRR"
     gene            complement(3110..3814)
                     /locus_tag="PK610_03930"
     CDS             complement(3110..3814)
                     /locus_tag="PK610_03930"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF011369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HRJ35107.1"
                     /translation="MLRSRTSLAVLTILVVLLLDQWLKIWVKTHMILGESIHITDWFQ
                     ILFTENPGMAFGMQPGGEGSYWGKLFLSVFRLIACFFIGWYILTLIRKNERKLLIFSM
                     ALIWAGAFGNIIDSMFYGILFSDSFLFPAEFLPADGGYAGFLHGYVVDMFYFPLFTGH
                     FPEWFPFWGGQEFLFFRPIFNLADASISIGVVILIIRQKTFFGQHAKKKHKEEAEENQ
                     ETVNETPAQDASVSAE"
     gene            complement(3822..4223)
                     /locus_tag="PK610_03935"
     CDS             complement(3822..4223)
                     /locus_tag="PK610_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRJ35108.1"
                     /translation="MVNKKPAEVDTRTRFSDAELQEFREIILKKLDEARRDLELLRGS
                     LSHTDDHGTDDTSPTFKMMEDGSETLSREETAQLAGRQEKFISALESALVRIENKTYG
                     ICRVTGKLISKDRLRLVPHTTLSIEAKNLQN"
     gene            4663..4926
                     /locus_tag="PK610_03940"
     CDS             4663..4926
                     /locus_tag="PK610_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35109.1"
                     /translation="MAAAFFAGAAFFAGLAAAFLTGAFLAGAAFFAGLDVGFLATGAF
                     FAGAFLAGAAFLTGFAAFLAGAAFLTGFFAAAFAGFFFAAITN"
     gene            complement(4928..>8343)
                     /gene="ileS"
                     /locus_tag="PK610_03945"
     CDS             complement(4928..>8343)
                     /gene="ileS"
                     /locus_tag="PK610_03945"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRJ35110.1"
                     /translation="KGKVFERSVSTREGKTPYVFYEGPPSANGMPGIHHVMARSIKDI
                     FCRYKTLKGFQVKRKAGWDTHGLPIELGVEKELGITKEDIGKNISIAEYNEACRKAVM
                     KYTDVWNDLTRKMGYWVDMDHPYVTYENKYIESVWWLLRNLYDKGLLYKGYTIQPYSP
                     AAGTGLSSHELNQPGCYRNVKDNSAVAMFEVKQDGFSSLLKAFKAENSGIGNASILAW
                     TTTPWTLPSNTALAVGPNIEYVLVQSYNAYSHQPIAVVLAKDLVTKHFSDKASELKLE
                     DYKPGDKLIPWKVISACKGSDLVGIRYQQLLPYALPHENAEQAFRVIPGDFVTTSDGT
                     GIVHIAPTFGADDMRVAREAGVPPMLVLDEKAKAVPLVDLRGRFRQELNDPTFGLGGE
                     HVKEDYLSAEEKAAELQKQKELLKGIIADTSKLNYLSVDERIILKLQNEGKLFKKEKY
                     EHNYPHCWRTDKPILYYPLDSWFIKATAAKDRMIALNKTINWKPEHTGTGRFGEWLNN
                     LNDWNLSRSRYWGIPLPIWRNEEGEEKCIGSAEELKAECDKAIAAGFMKENPLKNFHP
                     GNFSEENYNSFDLHRPYADDIVLVSASGKPLKREADLIDVWFDSGAMPYAQLHYPFEN
                     KELIDNKTNYPADFIAEGVDQTRGWFYTLHAIASLCFDSVAYKNVVSNGLVLDKNGQK
                     MSKRLGNAIDPFSTLEKFGPDATRWYMITNAAPWENLKFDLDGITETQRKFFRALYNT
                     YAFFALYSNVDGFCYKETEIPLEKRPEIDRWILSRLNSLVKSVDQSYAEYEPTRAGRE
                     IEEFVLDHLSNWYVRLCRRRFWKGEYSEDKIAAYQTLYTCLETIAILAAPIAPFYSDQ
                     LFQDLNRVSKKHTAESVHLADFPTANEKAIDLYLEEKMGLAQRITSMVLALRKKQDIK
                     VRQPLARIMVPVLNSTFREQLQSVEELVLSEVNVKALEYLDQNNAILVKRIKPNFKTL
                     GKKYGKHMKAISAKVAEFGQEEIAGIEREGRFSFVTEGETIILELEDVEIQSQDIPGW
                     LVASEGALTVALDINISTELHQEGLAREVVNRIQNLRKESGLELTDKISVKIQRHNTL
                     NDAITNNLNYICSETLANTLELVDSVNGNGVEVEVDETIKTSILIEKER"
BASE COUNT         2018 a         1846 c         2007 g         2472 t
ORIGIN      
        1 ggttgagaat gttttgtact ccaagtacat tcaccaggtc aagcgcaatt tcatgcgtga
       61 tcttttttgc gttgatcttg taattgatct ttaaatcgaa acggtaatag ggacgaaact
      121 gcatggagtt gttaccgtct ttacgaaaga taagctcgtt gttcgttgca gaggccagag
      181 aatctacata accgtgccag cggccgcctg cggtggtgaa ttttgcgcca agtccaatgg
      241 tggatttttt attgatgctg aattcttttc caaaaaggaa attagctgca tattttgtat
      301 tgtaatcggt attacgcagg gtgtcgtcgc taccgcggta ttttgcatcg aaaacagaac
      361 cggtaaccaa aaagaagaaa cttttattga agaatttttc aagtgtaact tccactccgt
      421 agttgcgtgc ggtgcctttg tttaccaatg tatccggaaa gaatcgggaa aatccggaac
      481 ccatgttggt cagtgaaaac gaacttggat tttgctcaac aggtatttcg tacaggtatt
      541 gataataggt ttctgctttc aaacgcatgg tgggattaaa tgcataatcg tatcccaaaa
      601 cagaatgaaa acttttggta aagcccatac ctttgttgtg gggtgctttc agacttccat
      661 ccggattttg gtaccggtag aaatacgtgt aatagggttg tgtttgcgaa tggagccctg
      721 tcccgaagaa tactgcgctt ttgttgttga tggtataacg cattccgatt cgcggctcaa
      781 tgggtgaaat actttgcgag atgctaaaga actgactgtg caaaccagct gtcattgcca
      841 gtttttcgtt agggcgccat ttccattgga ggtaggcttg tccgagtact ccgtattcat
      901 cgttttgcca acggtgtttc cactgatcgt cgataaaact tactgcagaa tcccacatgt
      961 taaaccgaat catgttgagg ttgattccgt agcgcaatac atgctgcttg ttaagtttgg
     1021 tgttaaagct caccatgccg gttgtgcggc tcaccctgaa gcggtaattc aggatctggt
     1081 agatggagtc caccacaaat agattgctgg cagcatcgac gtgacgaagg atgtaaaaat
     1141 gttcagcgcg gttttgttcg tgtgcctgtg caatacccac acgcatgaat gttttttcgt
     1201 tgatggtttt cgtgtagctt attcccgtca tgcccatttg tgtgcggaaa tactggtcgc
     1261 gatcgtcttc accgtaaaaa tctgctttcg gcgctgtctg gttggaaata agaatatcga
     1321 tcagcgattt acctccaata ccgaaaaagg agaggttgcc cccttttttt gttgggaaat
     1381 tgaacttgaa ggagaggtcc tggtagcgcg gaacggcatc ggtacccaaa ctaattccca
     1441 aggattggaa aagactcaat gtggagtagc ggtagaccag catatacgag gaaccggttt
     1501 ttttattgat gggtccttct gcagcgagtt ctgttccgag gaatccgagc tgaccggaga
     1561 attcgtgttt ttggttgttc ccaggacgca tgcgcaaatc gaaaacggct gaagtggaat
     1621 tgccatattc agcaggaaat gcggaggtaa aaaaatcaga attcgccagt actttattgt
     1681 tgagcacact caccggtcct cctgcagaac ctgcaatagc gaagtgattg gggttgggca
     1741 gatcaaaacc ttcgaatttg taaaggaccc ccaatggcga gtttcctctt actacaatat
     1801 cgttccgcga atcgtctgca ccttgtacac ctgcaaagtt ggaagccata cgcgcagggt
     1861 cgccacgtga accggcgtaa cgttcggttt cggcaacatc aaatgatcgt gtgctggtga
     1921 gtgccatgtc gttgtttacc tccccacgtt gtctcgcaac cacttccacc tcagcaatgg
     1981 tcgttgccga ttcttcaagt tcaatggata atacaatttc tttggcggag cttacttcaa
     2041 tgtcgagcac tttatccata tatccgataa aactgatctt gacggatctt tttccgacgg
     2101 gcacaccttc tattctgaat ttgccatcag catcggttga tgccccgctc tttccggtgc
     2161 tatcgcccaa aatgcgcaca ttggctccag gtaatggcat ttgggtttcc ttgtcaacca
     2221 cctttccccg aatggtttgc acaggctgtg ccattccatt tagactgagc aatgtgaaaa
     2281 acatcaatag agaaaaataa cgaaccatag tacttatgat ttgggtttta cgcttcaaga
     2341 atacattatt tgagggctct acagaattga attttacagt tggacggatg aatttattca
     2401 atttcgcaaa tattgcgcgc acagtcttac ttttaccgca tgaaacgact ctccaacctt
     2461 attttttacc ctgcattatt aatcggtgtg gtgtatgccc tgtatttgtg gttttcgggt
     2521 caaccgatag gaagcggaga gtttgtgtat accggcgacc caaatcgctg gaccgatgta
     2581 tttttcaaag tgatattcgg acttgtgctc ggagcttatt tggtgaatgt tgtggacagg
     2641 tattccggat tgcttttttt cctgcgtgcc gaaaaaaacg aaaagatcct tcataatgca
     2701 gtactcggct cgcaggcctt gcgttggagc ggaatgacat ttggaatcgg aattcctctg
     2761 gcattattgt gtggcggatt cttaatgata gccgatacac atagttatct tgcaggtgtg
     2821 gttgtggtgg tttctgcact ggagaaactg atttattttg ttgtttgtcg cagcaaaaaa
     2881 ctattccgcg cagcattaac acctaatgct ttagttattc ataatggcgg cctgcagatt
     2941 ttttcctttg gcggattacg ctcaatagac agtaaatacg acgagttgta tttcgtgtat
     3001 aacgaaaatg atatacgttc ggtatttcgc tatttaacag aggagaaaga gcgtgaagct
     3061 ttttttcacc atcttgaatt gatggcgaag gacaagaaca ttcctgtttc tattcggcgc
     3121 tgacagaggc atcctgcgca ggggtttcat ttaccgtttc ctgattttct tcggcttcct
     3181 ctttgtgctt ctttttggca tgctgtccga agaatgtttt ctgccggata atcagaatca
     3241 ccacaccgat actgatggat gcatctgcga ggttaaagat cggtcggaag aagagaaact
     3301 cctgtccacc ccaaaacgga aaccattccg gaaagtgtcc ggtgaagagt gggaaataga
     3361 acatatccac cacataacca tgcaggaaac cggcatatcc accatcagca ggtagaaatt
     3421 cggcaggaaa gagaaagctg tcggaaaata agatgccgta gaacatggaa tcgataatgt
     3481 ttccgaatgc accggcccaa atgagcgcca tactgaagat gagcagtttt ctttcgtttt
     3541 tccggatgag ggtgaggatg taccaaccaa taaagaagca ggcgattaat cggaaaacag
     3601 agagaaagag tttgccccaa taacttcctt cgccaccggg ttgcatccca aatgccatcc
     3661 ccggattctc ggtaaagaga atttggaacc aatccgtaat atggatggat tcaccaagga
     3721 tcatgtgggt ttttacccag atcttcagcc attggtccag caaaaggacc acaagaatag
     3781 taaggacagc gagtgatgtc cgcgaccgta acaaagcgct attaattctg caggttctta
     3841 gcttcgatgc tcagcgtagt atgaggcacc agacgaagac gatctttaga gatcagctta
     3901 cctgtaacgc ggcagattcc gtaggttttg ttctcgatac gtaccaaagc actttcaaga
     3961 gcagaaatga atttctcctg acgtccggca agttgagctg tttcttcacg ggagagtgtc
     4021 tccgaaccat cttccatcat tttaaatgta ggagaggtat cgtcggtacc gtggtcatca
     4081 gtatggctca atgatccgcg caacagttca aggtcgcgac gtgcttcatc aagttttttg
     4141 aggatgatct cacggaactc ctggagttca gcatcagaga agcgggtacg ggtatctact
     4201 tcagccggtt ttttatttac cacaacagca ggtttttgcg gcttggtata aagcggaatg
     4261 gagattccac cgttaacggt cattaccgtg tctccggttg gagtaatgcg aatctctttt
     4321 gaagtaggat tatgtttttt tggaagaggt tttgccggtg gagcaggctt tgaagctgct
     4381 tttggagcag gggcaacagg ctttgcaggc gcatcttgtt tagctgccgg ttttgcagac
     4441 tcagccggtt tagccacagg tgcttttgca gccggagccg gtttagcagc cggagctgct
     4501 ttttgaggtt ctggtttttt tgcttccggt tttttgggag tcggagccgc ctttgcagga
     4561 gttttagcta caggagccgc cttttttaca ggctttgcag gtgcttttgc agccggagcc
     4621 gccttcttaa cgggcttagc cggagctgct ttcttcactg gtttggcagc tgctttcttt
     4681 gcaggagctg ctttttttgc gggtttggct gcggccttct tgaccggagc ttttttagca
     4741 ggagccgctt ttttcgcggg cttggatgta ggttttttgg ccactggagc tttttttgcg
     4801 ggggcttttt tagcaggagc tgctttctta acgggcttcg ccgccttctt ggcaggtgcc
     4861 gctttcttaa caggtttctt tgctgctgct tttgccggtt ttttctttgc cgccataact
     4921 aattgattta gcgttctttc tctattaaaa tactggtttt gatagtctcg tcaacctcaa
     4981 cttccacgcc gttaccatta acagaatcaa ccagttccag cgtgttggcc aatgtttccg
     5041 agcaaatata attcaaattg ttcgtaatcg catcgtttag ggtgttgtga cgctgaattt
     5101 taacgctgat tttgtcggta agctccagtc ccgattcttt gcggaggttc tgaatgcggt
     5161 ttaccacctc cctggccagt ccttcctggt gcagctcggt gctgatgttg atgtcgagcg
     5221 ccacggttaa ggcaccttcc gaagctacca accagccggg gatgtcttgc gactgaattt
     5281 caacgtcttc caattccaga atgatagttt ctccctcggt cacaaagctg aatcttcctt
     5341 cacgctcgat tccggcaatt tcttcctgac caaattccgc aacttttgca gaaatggctt
     5401 tcatgtgctt accgtatttt ttacccaatg ttttaaaatt gggtttgatg cgttttacca
     5461 gaatcgcatt gttctgatcg aggtattcca acgctttcac attgacctcg ctcaatacca
     5521 gttcttcaac agattgcagt tgctcgcgga aagtggagtt gagtacgggc accataatgc
     5581 gtgcaagggg ctgacgaacc ttgatgtctt gttttttcct tagcgcaaga accattgagg
     5641 tgattcgctg agccaatccc attttttctt cgagataaag atcaatagcc ttttcgttcg
     5701 ccgttgggaa atctgccaga tgcaccgact ccgccgtatg ttttttgctt acacggttga
     5761 ggtcctgaaa gagctgatcg gaataaaacg gcgcaatcgg agcagccaga atagcgatgg
     5821 tttccaaaca ggtgtacaat gtctggtagg ctgcaatttt atcttcactg tattctccct
     5881 tccagaagcg gcgacgacat aaacgaacat accaattgct caaatgatca agaacaaatt
     5941 cttcgatttc ccttcctgcg cgcgtgggct cgtattcggc gtaactctgg tcgacagact
     6001 taacaaggga gttgagtctt gatagaatcc agcgatcgat ctccgggcgt ttctcaagcg
     6061 gaatctcagt ttctttatag cagaaaccgt cgacattgga atacaatgca aaaaacgcat
     6121 aggtattata caatgcacgg aaaaactttc gctgcgtttc tgtaatgccg tcgaggtcga
     6181 atttcagatt ttcccaggga gcagcattgg tgatcatgta ccagcgtgtg gcgtcgggac
     6241 caaacttctc cagcgttgaa aacggatcaa tggcattgcc caaacgtttc gacattttct
     6301 gtccgttctt gtcgaggacg agcccgtttg aaaccacatt tttgtaagcg accgaatcaa
     6361 aacaaagtga agcaattgca tgtagggtgt aaaaccaacc acgcgtttgg tcaacacctt
     6421 cggcaatgaa atcggcggga taattcgttt tgttatcgat taattctttg ttttcgaaag
     6481 gataatgcaa ttgcgcatag ggcatagctc cggaatcgaa ccaaacgtca attagatccg
     6541 cttctcgttt caggggttta ccgcttgcag aaacaagtac aatatcatcg gcatacggac
     6601 gatgaagatc aaaggagttg tagttttctt ccgaaaaatt gccgggatga aaatttttca
     6661 gtggattctc tttcatgaaa cctgcagcga tggctttatc gcattcagct ttcagttctt
     6721 ctgcagagcc gatgcatttc tcttcgccct cttcatttct ccagatggga agcggaattc
     6781 cccaatagcg tgaacgggaa agattccagt cgttgaggtt attgagccat tcaccaaatc
     6841 gtccggttcc ggtgtgttct ggtttccagt tgatggtttt gttcagcgca atcatgcgat
     6901 cttttgctgc agtggctttg atgaaccaac tatcgagcgg ataatacaga atgggtttat
     6961 cggtcctcca gcaatgcggg tagttgtgct cgtatttttc tttcttgaat agttttcctt
     7021 cgttctgcag tttcagaatg atgcgctcat ccacgctcag gtagttgagt ttggaggtat
     7081 cggcgatgat acctttgagc agttcttttt gtttttgtag ctcagctgct ttttcttcag
     7141 cgctgaggta gtcttccttt acatgttctc cacccaaacc aaatgtcgga tcattcagtt
     7201 cctgacgaaa acgtccgcgc aaatctacaa gcggaacagc ctttgctttt tcatccaaca
     7261 ccagcatagg agggactccg gcttctcggg ctacgcgcat atcgtcggca ccaaatgttg
     7321 gagcgatgtg tacgattccg gtaccgtctg aagtagttac aaaatcaccg ggaataacac
     7381 gaaaagcctg ctctgcgttt tcatgcggca aggcgtaggg aagaagctgc tggtaacgga
     7441 tacccaccag atcagaacct ttgcaggcag aaataacttt ccagggaatg agtttatcac
     7501 ccggtttata gtcttccagt tttagctcgc ttgctttatc actaaagtgt tttgttacga
     7561 gatcttttgc aagcaccacc gcaatgggtt gatgtgaata agcattgtag gattgaacca
     7621 aaacatactc aatgttcggt ccaacggcga gagcagtatt ggaagggagt gtccagggag
     7681 tggttgtcca tgcaaggatg gaagcgtttc caatgccgct gttttctgcc ttgaacgctt
     7741 taagcaagga tgaaaaacca tcttgtttaa cctcaaacat cgctaccgcc gaattgtctt
     7801 ttacgttgcg gtaacaaccg ggctgattaa gttcgtgcga tgagagtcct gttcctgctg
     7861 ccggcgaata aggctgaatg gtgtagcctt tgtagagtaa tcctttgtcg tagagattgc
     7921 gcagcagcca ccagacggat tcgatgtatt tgttttcgta ggtgacatac gggtgatcca
     7981 tatccaccca ataccccatt ttacgggtaa ggtcgttcca cacatcggtg tacttcatta
     8041 ccgcttttcg gcaggcttcg ttgtattcgg caatgctgat gtttttgcca atgtcttctt
     8101 tggtaatacc caattccttt tccacgccaa gctcaatggg gagcccgtgg gtatcccatc
     8161 ctgctttacg ttttacctga aatcctttga gtgttttata gcggcagaaa atatccttaa
     8221 tggatcgggc cattacgtgg tgaatccccg gcattccgtt agcggaagga ggaccttcgt
     8281 aaaacacata aggggttttc ccttcgcggg tgctcactga gcgttcaaaa accttcccct
     8341 ttt
//
LOCUS       DAOMYG010000081         8326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2855_length_8326_cov_8.422561, whole genome shotgun sequence.
ACCESSION   DAOMYG010000081 DAOMYG010000000
VERSION     DAOMYG010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8326
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2855_length_8326_cov_8.422561"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="PK610_03950"
     CDS             <1..930
                     /locus_tag="PK610_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRJ35111.1"
                     /translation="NHDYVLIFTEKGKVFWIRAYEIPEGEKTSKGRAIQNLINIDGED
                     KVRAYINVKDVTDPAYFADKSIILCTKRGVIKKTELEAYSRPRSNGINAITIRENDTL
                     LEARLTNGKDHIMMALNTGRAILFQEDEVRDMGRTASGVRGIRIEDETKEEVVGMVTF
                     GNIDEEEVLVVSEKGYGKRSKVSDYRITGRGGKGVKTLNITDKTGQLVAIKNISDDND
                     MMIITKGGVTIRINASELRTMGRVTQGVRLINLKNSESIASVAVVNKGDEEEEAAEIA
                     IEGTNPDSPIVNEGENPATDAGTEIENNTPDVE"
     gene            970..2232
                     /locus_tag="PK610_03955"
     CDS             970..2232
                     /locus_tag="PK610_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ35112.1"
                     /translation="MKNLRNLTLGLLLAGLCFTGVDAQVNNRVSAKNAMDDFGKLQQS
                     NPAGAKNKLLEAKKYIDLAAAHDETKNDPKTLFYKGQIYFYVSMLSGAKDAQFVEFDA
                     EATGKEAFAAWKSAMANKNKKYDPKEDIYRMIFVPHTMSYQMGAKAFEKQKYDSAALY
                     FESCYELMNVMEVLDTLSCFNAGLAYDYHGEALTKKCAADKSTDCPEANKMYNKSADM
                     FKICADAGYQGSDLISRYAEVLTKAGKKDEAKAVIQAGRAKYPKNSALAIAEFNYYLS
                     IGDNEGAEKALAEAIKINPKDALLYFNAGAIYDEMKRYDDAKKAYENAIAINPNYFDA
                     YFNLGAMYYNQAVDIYKAINDIKDNAVYDKEKARADALFKEAIAPLEKARTLNSKDKN
                     NLIMLRTIYARLNMQEKWSEVDKAIKNG"
     gene            complement(2540..6124)
                     /locus_tag="PK610_03960"
     CDS             complement(2540..6124)
                     /locus_tag="PK610_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ35113.1"
                     /translation="MFRKGVIVFLLSGIPRLVFAQPSSLFMDATVEWVNVGDLDVAGD
                     QLTVEALIHYTGASVNIVSKHTDPSNVNYLLRIGSFEITTTAGFANFGGVAAAGVTLS
                     PGTTYHVAATYNGQFLRYYVNGCLTGEMPWTGTMAQNNLATAIGQQSACQCEQFLGYI
                     DEVRIWNVARTQAQIRDNMLDLPTPTLQPGLLAYYKFDGNSVNLQGNATFNGTVQGAA
                     AFQQIPYLYPSSLNQTVSSSNPVCDGDANGAINVQTSGAYTPYQYSIDGLNFQASPNF
                     TNLPAGNYTVVSRPQNNANCITTSAIQITDPLPITSNLQTTQITCFGANNGSASVNPS
                     GGDGPLYRVQWSTGQTGNSISNLGPGNYSVAITDSCARSGPELVQNGAFQNGTSGFTS
                     DYTSCNNCYNGFNDIPSGAFVVSHNSNLHHSGFNGLGNGGQGNFMIVNGSDVPNTNVW
                     CQTINVQPDTYYFFSTAVSSVVAASPAQLQFEVNGALLGPVFTAPAAAGVWSTFSSVW
                     YSGPSTTAVICILNQNTTLGGNDFGLDDISFKACISCTESFPFTITEPSQLQVNVSGT
                     DVSCNGGNDGTAQAVPSGGTGLIGYSWNTIPVQNTNTINGLTQGNYSVTVSDANLCTA
                     TGTVVIGFVSDPQITAIVDTDIDCSTPNGGSITITATGNNLQYSIDGGLTFQPSNSFT
                     GLNSGNYAIVVQENTCQQTANATITASGLLSIITNQTDVSCPGIADGSATVNILSGNG
                     NYTFNWNTVPIQNTQTINGLNQGTYTVTVIDGNGCNASSNVNIGVQANPQITAIASTD
                     IQCFGGSNGTITVTASGTALQFSIDGGLTFQASNQFNGLAAGVYPVLIQQNTCQDNGF
                     ATINEPDQLELDIATTNTTCNGQCNGVVVATVTGGVLDYNYGWSNNLAGAADVQASGV
                     CAGNYSITITDANGCTVNQQFSITEPPALEIDNAFISNETCLNDCDGSIDLTAQNAVN
                     YQLGTNPQQPGGSFGNLCPGTYSVTLADIEGCTLNQNFTILPGTTVQASFDADRFIVS
                     ELEPYVNFSNTSTGAVSFDWDFAAAGTSALPSPGFRFTGFLPGIYPVCLEVSNQQGCR
                     DSICRNITVREDLLVYVPNTFTPNFDGKNDVFKPVCLACIHSDYNFRIYNRWGEIVFQ
                     TEDPTAGWDGTLSGGIRAQSGTYVWHMQFMLDGDVVVHTMRGHVNLLR"
     gene            6367..7350
                     /locus_tag="PK610_03965"
     CDS             6367..7350
                     /locus_tag="PK610_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35114.1"
                     /translation="MKHLYFLIIFGISVIPGMGQNFPNDGTLGGWATASAHDKFSKDA
                     ITTSFKDADTTQPLPIYFGDTLKPIPIAYMPKGDSIGSYVLPPGLYEMTVQSYCIKAG
                     TYGPSQGDGYCYAALNGPQKNLMISALTNAKKHPRLLQQDVQVLLWAIIARARFNDMN
                     ERLKVITLTLLNAKEILQMNKTGLGLIPKPFLAKMATQLPPLVYEIVRAENQMRQLFY
                     NANTVYSEYERWALRSGMAPVDRPDIRRGRWCKHPCGYYIRFYPQSYNKTKVQVYVPE
                     DFSSRANPPCSVRTKYEGAELHGVLFNLLGGVAVPANTQSQRLLLSNTEAL"
     gene            complement(7351..7797)
                     /locus_tag="PK610_03970"
     CDS             complement(7351..7797)
                     /locus_tag="PK610_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HRJ35115.1"
                     /translation="MNKQECWMTCKVVLEQKLQDILHSLAEIQESANAETKSSAGDKH
                     ETARAMAQNEIELASVQLENTRQMLRDLERIDPAQSKPIIGSGSLVKTDTAVYFIGAA
                     IGKLNSSGSLVYAISPQSPIAREMLGKRAGDHFQFNGKEIAIRAVD"
     gene            complement(7810..8247)
                     /locus_tag="PK610_03975"
     CDS             complement(7810..8247)
                     /locus_tag="PK610_03975"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRJ35116.1"
                     /translation="MKKAIIHTEKGDMKVEFFHADAPNTVANFIKLSESGFYKGLSFH
                     RVIPNFVIQGGCPNGIGNGGPGYKIDCELTGGNQFHDRGVLSMAHAGRNTGGSQFFIC
                     HSRANTAHLDRNHTCFGKVVEGLEIIDQIRQGDKIVDIEIVEA"
BASE COUNT         2443 a         1808 c         1828 g         2247 t
ORIGIN      
        1 aaccacgact atgtactcat ctttactgaa aaaggaaaag tattctggat tcgtgcttac
       61 gaaattcccg aaggagagaa aacgagcaaa ggacgcgcaa ttcagaacct cattaatatc
      121 gacggtgaag acaaagtgcg tgcctatatc aatgtaaagg atgtaacaga tccggcgtac
      181 tttgccgaca aatccatcat tctttgtacc aagcgcggtg taatcaaaaa gaccgagctc
      241 gaagcttatt cgcgtccacg ctccaacggg atcaatgcca ttactatccg tgaaaacgac
      301 acacttctgg aagcacgtct taccaatgga aaagatcata ttatgatggc actcaataca
      361 gggcgagcca ttcttttcca ggaagacgaa gtacgtgata tgggaagaac agcttcgggt
      421 gttcgcggta tacgtattga ggacgaaacc aaggaagagg ttgtgggaat ggtcactttc
      481 ggaaacattg atgaggaaga agtccttgta gtatctgaaa aaggatacgg aaaacgttcg
      541 aaagtaagcg attaccgtat tacaggtcgt ggtggaaaag gtgtaaaaac actgaatatc
      601 accgataaaa ccggtcagct ggttgccatc aagaacattt cggacgataa cgatatgatg
      661 attattacca aaggtggagt aaccattcgt atcaatgctt ctgagcttcg cacgatgggt
      721 cgtgttaccc agggagtacg cctgatcaat ttaaagaaca gcgaatcgat tgcttctgta
      781 gccgttgtta ataaaggaga tgaagaagag gaagctgcag aaattgcaat tgaaggcacg
      841 aatccggata gtccgatcgt taatgaagga gaaaatcctg caacagatgc tggcacggaa
      901 attgaaaata acactccgga tgtggaataa ttgaaaggac tgcatcctta aaacagaaaa
      961 agaaataaaa tgaagaacct acgcaacctt acactcggac tattgctggc cggattgtgt
     1021 tttaccggag tcgatgctca ggtaaataat cgtgtttcag ctaaaaatgc aatggacgac
     1081 ttcggtaaac ttcagcaaag caatcctgcc ggagcgaaga ataaactgct ggaagccaag
     1141 aaatacattg acctggccgc tgcgcatgat gaaacgaaaa acgatccgaa aaccttattt
     1201 tataaggggc agatctattt ttatgtttcc atgctttcag gcgcaaaaga tgcacaattt
     1261 gtagaattcg atgccgaagc taccggtaaa gaagcctttg cagcctggaa atcggcgatg
     1321 gccaataaga acaagaagta cgatccgaaa gaggatatct accgtatgat ctttgtgccg
     1381 cataccatga gttatcaaat gggagcaaaa gctttcgaaa agcagaaata cgattcggca
     1441 gcattgtatt ttgaatcgtg ttacgagctg atgaacgtta tggaggttct ggataccctg
     1501 agctgtttca atgcaggact ggcttatgat taccatggcg aagccctcac caaaaagtgt
     1561 gcagccgata aaagcacaga ttgtccggaa gccaataaga tgtataataa gtccgccgac
     1621 atgttcaaaa tctgcgccga tgcaggttac cagggatcag accttatcag ccgttacgca
     1681 gaagttttaa ccaaagcagg caaaaaagac gaagctaaag cagtgattca agcgggtagg
     1741 gctaaatacc ctaaaaacag cgccttggct attgcagaat tcaactatta tttatcgatt
     1801 ggcgacaatg aaggagctga aaaagctttg gcagaagcca ttaaaatcaa ccctaaagac
     1861 gccctgttgt actttaacgc aggtgctatt tacgatgaaa tgaagcgtta cgatgacgcc
     1921 aaaaaggctt acgagaacgc gattgctatc aatcctaact attttgatgc gtattttaac
     1981 cttggtgcga tgtattacaa ccaggctgtt gatatttaca aagccattaa cgacatcaag
     2041 gacaatgctg tttacgacaa ggaaaaggcg cgtgcagacg cattgtttaa ggaagcaatt
     2101 gctccgcttg aaaaggccag aacccttaat tctaaagaca aaaacaacct tattatgttg
     2161 cgtactatct acgcacgttt aaacatgcag gagaaatggt cggaagttga taaggcgatt
     2221 aagaacggtt aataccatat taaatattag cacaaaaggc ggtccggttg gatcgccttt
     2281 tttatttgct gctttacgat ggaagagtgt attggtaagt tctttgttag tagtcagagg
     2341 ttaccagtta gttggacact tcggcaaatt ctgtgcttgt ggaaggatgg aagtagactc
     2401 atcccaagtt tagcacataa ggaagttaat tgactataaa ttactccttt tgaattactt
     2461 acaacttaac ataatgtaaa ttataagtaa tttaagcgat aataattttg gtcatagctc
     2521 ccagacataa ataatcggct cacctcaaaa gattcacatg tccgcgcatg gtgtgtacaa
     2581 ccacatcgcc atcgagcatg aactgcatat gccagacata ggtcccggat tgagcacgaa
     2641 tcccgccact aagcgttccg tcccagccgg ctgttgggtc ttcggtctgg aaaacgattt
     2701 ctccccagcg gttgtagatt ctaaagttgt aatcggaatg aatacaggcc agacaaaccg
     2761 gtttgaacac atcgtttttt ccatcgaaat tgggggtaaa tgtattgggc acatacacca
     2821 gcaaatcttc gcgtacagta atgtttctgc agatagaatc cctgcatcct tgttggtttg
     2881 acacctccag acaaacggga tatatgcccg gtaaaaagcc cgtaaaacgg aacccgggac
     2941 ttggtaaagc gcttgttcct gctgctgcaa aatcccagtc gaaagaaact gcaccggtgc
     3001 tggtattact gaaattaaca taaggttcta actcgctgac aataaaacga tctgcatcga
     3061 aagaagcctg aacggttgta cccggaagaa tcgtaaaatt ctgattaagt gtacagcctt
     3121 caatatcggc aagtgttacc gaataggttc ccggacagag atttccaaag cttccaccag
     3181 gctgttgtgg gtttgttcct aattggtaat tcacagcatt ttgagcagtt aaatcaatgg
     3241 aaccatcgca atcattcaaa caagtttcat tgctgataaa ggcattatca atttccaaag
     3301 ccggtggttc agttatcgaa aactgctgat ttacagtgca tccattggca tcggtaatgg
     3361 tgattgaata atttcctgca cacacaccac ttgcctgcac atcggcagct ccggccaggt
     3421 tattgctcca tccgtaattg tagtccaaca cccctcctgt tacagttgcc accactaccc
     3481 cattacactg accattacaa gtggtattgg tagtggcaat atccaattcc agttgatcgg
     3541 gttcatttat cgttgcaaat ccgttatcct gacacgtatt ctgctgaatg agtaccggat
     3601 aaacccctgc agcaagtccg ttgaattgat tcgatgcctg aaaagtaaga cctccgtcga
     3661 tgctaaactg caatgctgta ccgcttgctg taacagttat tgttccgttc gaacctccga
     3721 agcattggat atctgtgctt gcaattgccg tgatctgagg attggcctga accccaatat
     3781 tcacgttaga gctcgcatta caaccattgc cgtcgattac agtaacagta taagtacctt
     3841 gatttaaacc gttaattgtc tgtgtgtttt gtattggaac agtgttccag ttgaaagtgt
     3901 aattgccatt accgcttaga atatttacgg tggctgagcc atctgcaatc ccggggcagg
     3961 aaacatcggt ctggttggtg ataatactca ataaaccact ggccgtaatg gtggcattag
     4021 cggtttgctg acaagtattt tcttgtacta caatggcgta atttcctgaa tttaaacctg
     4081 taaatgaatt ggaaggctgg aatgtcaagc cgccgtcgat actgtattgc agattatttc
     4141 cggtagccgt aattgtaata cttccaccgt ttggtgtaga acaatcgata tccgtatcaa
     4201 caattgccgt aatctggggg tcggatacaa agccaattac aaccgttcca gtggcagtac
     4261 aaaggttggc atcgcttaca gtaacactgt aattgccttg tgttaatcca ttgattgtat
     4321 tcgtgttttg aacggggatt gtgttccagg aatagccgat taatccagtg cccccggagg
     4381 gtacggcttg cgctgttcca tcattaccac cgttgcagga tacatctgtt cctgaaacat
     4441 tcacttgtag ctggcttggt tcagtaatgg taaaaggaaa actttccgtg caggaaatgc
     4501 aagctttaaa ggagatatca tccagaccaa aatcattccc gccaagtgtg gtgttttgat
     4561 taagaataca gataactgca gttgtggaag gaccagaata ccaaaccgaa gaaaaggtag
     4621 accacactcc tgctgcagcg ggtgccgtaa acacaggtcc gagtaaagca ccgttcacct
     4681 caaactgcaa ttgggcagga cttgcggcta ccactgagct tactgccgtt gagaagaaat
     4741 aataggtatc tggctgaaca ttaatggttt gacaccatac attggtatta ggaacatccg
     4801 atccgttaac aatcataaaa ttgccttgtc caccattacc aagtccgtta aaaccgctgt
     4861 ggtgaaggtt ggaattgtga ctaaccacaa aggccccact gggaatatcg ttaaatccgt
     4921 tgtagcaatt gttgcaggaa gtatagtcac tcgtaaaacc ggatgtcccg ttctgaaaag
     4981 caccattctg taccaattca ggtccggatc gggcgcaact gtcggttatt gcaaccgaat
     5041 aattacctgg accgagattg gagatggaat taccggtttg acctgtactc cactgtaccc
     5101 ggtaaagcgg accatcacct ccacttggat ttacggatgc acttccattg ttagccccga
     5161 agcaggtgat ttgtgtagtt tgaagattgg aagtaatggg caatggatca gtaatttgta
     5221 ttgcagaagt ggtaatgcaa ttggcattgt tttgtggtct ggacacaaca gtgtaattac
     5281 cggccggcaa atttgtgaaa ttgggactcg cctgaaaatt caaaccatcg atggaatact
     5341 ggtaaggtgt ataggccccg ctggtttgaa cattgattgc tccattggca tcgccatcgc
     5401 aaacagggtt tgaactactt actgtctgat taagtgatga tggatacaga tatggaattt
     5461 gctgaaaagc agcagctccc tgaacggttc cgttaaaggt tgcatttccc tgcaggttta
     5521 ccgaattccc atcgaattta taataggcca ataagccggg ctgtagtgtg ggcgtgggta
     5581 aatcgagcat attatcacga atctgtgcct gcgtacgggc aacattccag attctcactt
     5641 catctatata acccaggaac tgctcacatt ggcaagccga ttgttgtccg attgcagtag
     5701 ccagattgtt ctgtgccatt gtgccggtcc agggcatttc tcctgttaaa caaccgttca
     5761 cataataacg aaggaattgt ccgttgtagg tagccgccac atggtaggta gttcccggac
     5821 taagcgtaac tcctgccgca gccactccac cgaaattggc aaatccagcc gtggttgtaa
     5881 tttcaaaact tccgatgcgc aacaggtaat ttacattgct gggatccgtg tgtttactta
     5941 cgatattaac cgatgctccc gtgtaatgta taagggcttc cacggtaagc tgatctccgg
     6001 ctacatccag atcgccaaca ttgacccact ccacagttgc atccatgaac aaggaagaag
     6061 gttgtgcaaa aactaaacgc ggaattcctg ataaaagaaa aacaatgacc ccttttctga
     6121 acatttcaac gtattgattt agagtgcaaa tttacatctg aacgggccaa aaattgttct
     6181 tggattttca ataattatca acccaaactc aatccgttta gtaaatcgct tcaagtttag
     6241 aaatgccttt aatcaactag ttatcaatca caaagcggta attaagaaca agtttgtaat
     6301 tcggatattt actgaaaatc attcatggct cagattttgt ggaaaagttc tacctttaat
     6361 tgagttatga aacaccttta cttccttatc attttcggaa tatccgttat tccgggaatg
     6421 gggcaaaact tccccaacga cgggacatta ggtggttggg ctaccgcatc tgctcacgat
     6481 aaattctcaa aagacgccat tacaaccagc ttcaaggatg cagataccac tcagccatta
     6541 cccatttatt ttggcgatac cctcaagcct atcccaattg cctatatgcc gaagggtgat
     6601 tccattggta gttatgtttt gcctcccgga ttatatgaaa tgacggttca gagttattgc
     6661 atcaaagcag gaacctacgg accaagtcag ggcgatggat attgttatgc ggccttgaat
     6721 ggtccacaaa agaacctgat gatttcagcg cttaccaatg cgaaaaaaca tccacgactt
     6781 ttgcagcagg atgtgcaggt tttactttgg gcaattattg ccagagcacg gtttaatgac
     6841 atgaatgaac gacttaaagt aataaccctt actttattga atgccaaaga aatattgcag
     6901 atgaacaaaa ccggattggg tttgatccca aaaccttttc tggcaaaaat ggcgacccaa
     6961 ttaccgccgc ttgtttatga aattgtaagg gccgaaaacc agatgcggca actcttttac
     7021 aatgcaaata ctgtctattc cgaatacgaa agatgggcat tacgttccgg aatggcaccg
     7081 gttgaccgtc ccgatattcg tcgcggaaga tggtgcaaac atccttgcgg ctattatatc
     7141 cgtttttacc cacaatccta caataaaaca aaagtacagg tttatgtccc ggaggatttt
     7201 tcgtcaaggg caaacccacc ctgtagtgtc agaaccaaat acgaaggagc tgaattacac
     7261 ggcgttttat tcaatctgct cggtggagtt gctgttccgg ccaataccca atcgcaacgc
     7321 ttattgctga gtaataccga agccttatga tcaatccaca gcccgaattg caatttcttt
     7381 cccgttaaac tgaaaatgat ctccggcccg ctttccaagc atttccctag cgatgggaga
     7441 ttgcggactg atggcataca ccaatgaacc tgagctgttc aattttccaa ttgcagcacc
     7501 aataaaataa acagcagtat ctgtttttac cagacttcct gatccgatta tcggtttact
     7561 ttgtgcagga tcaattcttt ccagatcacg cagcatttgt cgcgtgtttt cgagttgaac
     7621 agatgccagc tcaatttcat tttgggccat tgctcttgct gtttcatgct tatctccggc
     7681 gctgctcttg gtttcggcat tggccgattc ctgaatttca gcaagcgaat gcaaaatatc
     7741 ctgaagcttc tgctccaaaa cgaccttgca ggtcatccaa cattcctgct tattcacaca
     7801 gaataatgat caggcttcta caatctcaat atctacaatc ttatcgccct gacggatctg
     7861 gtcgatgatt tcgagtcctt ccaccacttt tccaaaacag gtgtggttgc gatcgagatg
     7921 tgcggtgttt gctctgctgt gacaaatgaa aaattgtgat cctccggtat ttcttccggc
     7981 atgtgccata ctcaaaacac ctctgtcgtg aaactgattg ccaccggtca attcgcaatc
     8041 gattttgtat ccgggaccac cgttaccaat tccgtttgga caaccgccct gaattacgaa
     8101 gttgggaata acacggtgga aactcaatcc tttataaaac ccgctttcgc ttagtttaat
     8161 gaagttggca acagtattcg gagcatcagc gtggaagaat tccactttca tatctccttt
     8221 ttcggtgtga atgatcgctt ttttcataat tcaattttgt gctgcaaaga taacaagcaa
     8281 tttattcccc aagggaaaag tatttcgatc cgaatcgttc gtgaat
//
LOCUS       DAOMYG010000082         8321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2859_length_8321_cov_8.769417, whole genome shotgun sequence.
ACCESSION   DAOMYG010000082 DAOMYG010000000
VERSION     DAOMYG010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8321
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2859_length_8321_cov_8.769417"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1115
                     /locus_tag="PK610_03980"
     CDS             132..1115
                     /locus_tag="PK610_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ35117.1"
                     /translation="MVRIGVLGAGHLGKIHIRCIKEVPELELVGFYDPDDANAADATE
                     KYGAKRFTDIESLIDACDALDIVTPTTSHHDTAQRCLRKFKHIFIEKPITTTVDEAKS
                     LINLAKEANVIGQVGHVERFNPAFSASVPFFHQPMFIECHRLAVWNPRGTDVSVVLDL
                     MIHDIDIILSVVKSNIKRISASGVAIVSDSPDISNARIEFDNGCVANLTASRISLKNM
                     RKTRFFQRDAYISVDFLEKKTEVVRMKQIEGEPDPFAFMIDLGNNKGKREIFFEKPDV
                     KETNAIRDELQAFAQAILSGNNPPVTLQDGLNALDVAYKIIGKMEVTSGIS"
     gene            1160..2050
                     /locus_tag="PK610_03985"
     CDS             1160..2050
                     /locus_tag="PK610_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35118.1"
                     /translation="MERRLYVIIALIITGFVSCQKDQGEIPSYIYIPDIRLTTTSAQG
                     STSDDIIDAWVYVDDNPVGCFPLPARVPVLAEGNRKISVYGGIKADGISTRRRRYIFY
                     QPFVTNSFTLRRGMVDTLKGVFEPVVSYYPSNQITIWYEDFTDPFVPFFQDAISDTTI
                     NRITDPGEVFEGAGCGLLTLSATQNFVQVATSENFDLPKGGIPVYLELNYKANQAFAV
                     GLRSIYPGNILNQENTIIRDTYDDNGVLVWKKIYCEFTELVSSNINAISHEIFFRVYK
                     DQGVSVPQVYIDNVRLIYGN"
     gene            2040..3449
                     /locus_tag="PK610_03990"
     CDS             2040..3449
                     /locus_tag="PK610_03990"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRJ35119.1"
                     /translation="MAIDPKARQVLKYFLSDLITAGLAWTLFFVFRKTWIEQTVIEFD
                     ENYYRGLIIIPLFWVTLYYLSGYYSNIFRKHRIQDLGFTLIQTLVGVLFLFFVVILDD
                     EVRTYTSYYYAFFALFGVHFLLTFLPRIFFTSRLVRRIHKRKIGFNTLLIGGNANALN
                     IYREIMDMKQSPGYKFVGFVSINGVDDQLREEHLPYLGKYKSGIQEVLKNHKVEEAII
                     AIESSEHESLKKIISDLEGDQLRLHVIPDMYDILTGSVRMTNIYGTPLIEIRSQLMPA
                     WQASVKRMMDVLISSVSLLLCLPMFLVIAIIIKLSSKGPVFFLQDRVGINGRPFRIIK
                     FRSMVVNAESNGPQLSSENDPRITGIGRFLRKTRLDEFPQFINVILGDMSLVGPRPER
                     QFYIDKIMEFAPHYRYLNKVRPGITSWGQVKYGYAENVEQMIQRMKYDLIYIENMSIA
                     LDIKIMFYTLAIIFKGKGK"
     gene            3506..3652
                     /locus_tag="PK610_03995"
     CDS             3506..3652
                     /locus_tag="PK610_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35120.1"
                     /translation="MAVYDRFLVSNPGPEEKSGFGLYLSLFGAGVVMSLLLWRLAKTA
                     AKHL"
     gene            3713..4306
                     /gene="hisH"
                     /locus_tag="PK610_04000"
     CDS             3713..4306
                     /gene="hisH"
                     /locus_tag="PK610_04000"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRJ35121.1"
                     /translation="MEIVVIDYKAGNIRSVLFALERIGANGILTDDPQRILNADKVIF
                     PGVGEASSAMEALRSRGLDKLIPTLKQPLLGICLGMQLLCSHSEEGNTNCLNVFPVQV
                     KRFPEKRAGVEKVPHVGWNTVSGLKGALFNDLPDPVYMYYVHSFYAEQSAFTTGVSRH
                     IIDYSASLQKDNFYAVQYHLEKSGDAGERLLRNFIAI"
     gene            4373..5578
                     /locus_tag="PK610_04005"
     CDS             4373..5578
                     /locus_tag="PK610_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HRJ35122.1"
                     /translation="MSKVLIIGAGGVGQVATYKCAMNTDVFTEIVLASRTKSKCDKIA
                     EDIKAKLGVTIKTDQVDADNVAELIALFKKHQPKLVVNLALPYQDLTIMDACLATGTH
                     YMDTANYEPKDEAKFEYKWQWAYQEKFKQAGLTAILGCGFDPGVTGVYTSYAAKHHFD
                     EIHYLDIVDCNAGDHGKAFATNFNPEINIREVTQKGKYWQDGKWVETEPHEIHMPLNY
                     PNIGPKESYLIYHEELESLVKNFPTIKRARFWMTFGLEYLTHLRVIQNIGMARIDPII
                     YEGKEIVPIQFLKAVLPNPGDLGENYKGETSIGCRIRGIKDGKEKTYYVYNNCSHEVA
                     FSETGTQAVSYTTGVPAMIGAMMFLTGQWTGAGVWNVEQFNPDPFMEQLNKQGLPWHE
                     LHNVDLELN"
     gene            5622..6755
                     /gene="nspC"
                     /locus_tag="PK610_04010"
     CDS             5622..6755
                     /gene="nspC"
                     /locus_tag="PK610_04010"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HRJ35123.1"
                     /translation="MIDLNKIPAPCYVIEEELLRKNLTLIRDVSQRAGVEIILAFKGF
                     AMWSTFPIVREYISGATASSVYEARLCFEEMKTKAHSYVVAISPTEIDDLLDYSSVLT
                     FNSVSQFERYKQKCIDRGVSIGIRVNPEYSEVTTELYNPCSPNSRLGMTSEHFSDRLP
                     DGVDGLHFHALCENDSYTLEKTLVAFEAKFGKLIPQCKWVNFGGGHLMTRDGYDVEHL
                     ISVLQSFKSRYPGVKVILEPGSAFAWRTGYLVSTVLDIVENNGVKTAMLDVSFTAHMP
                     DCLEMPYRPRIWGATDPVKGKPTYRMGGTSCLSGDYMFEYSFEQELQPGNKIVFDDMI
                     HYTMVKTTMFNGVNHPSYGIWTSKGEFRLVRKFAYEDYKMRLS"
     gene            6789..7514
                     /gene="hisA"
                     /locus_tag="PK610_04015"
     CDS             6789..7514
                     /gene="hisA"
                     /locus_tag="PK610_04015"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010178528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HRJ35124.1"
                     /translation="MILIPAIDIIDGKCVRLTKGDYSTRKVYNEDPLEVAMQFQDAGL
                     THLHLVDLDGAKASHIVNWKVLEKIAARTKLHIDFGGGLKSDKDLQIAFNSGAEKITG
                     GSIAVKSPDVFKQWIKDYGTERIILGADVKEEKISITGWTEDTDTYVCDFLNDYRSSS
                     GIEYVICTDIAKDGMLEGPSMELYSKILSEIPSLKLIASGGVSSVSDLEQLKEKGLFA
                     AIIGKAIYEGKISLGELAKMVSA"
     gene            7719..>8321
                     /locus_tag="PK610_04020"
     CDS             7719..>8321
                     /locus_tag="PK610_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRJ35125.1"
                     /translation="MLTHRIIPCLDIKDGRTVKGVNFVDLRDAGDPVELAVQYAAQGA
                     DELVFLDITATNEKRRTLAELVRKIASLLNIPFTVGGGISSVEDVSILLESGADKIAV
                     NSAAVKRPELITELASKFGSQCVVVAVDAKKVEGKWLVHLNGGKLQTEIDLFDWVKEI
                     ERRGGGEILFTSMDHDGTKNGFANEALEKITSLVNIPVIAS"
BASE COUNT         2363 a         1681 c         1869 g         2408 t
ORIGIN      
        1 tctgtttaaa ggcgtcaggt atctcgtgca tcacttagcg acatttcact gttgaaaatt
       61 actctgttct gctgcaccat gccctcgctc atgaagtaat tttatcacga acattttcaa
      121 cactaattat tatggtaaga atcggagtgc tgggcgcagg acatttgggt aagattcata
      181 ttcgctgtat aaaggaagtg cccgaactgg agctggtggg attttacgat cccgatgatg
      241 ccaatgctgc cgatgcaacc gagaaatatg gcgcaaaacg attcaccgat atcgaatcac
      301 tcattgatgc ctgcgatgca ctcgatattg ttacaccaac aaccagtcac cacgataccg
      361 ctcaacgttg cctgcgtaaa ttcaagcata ttttcattga aaaaccaatc acaacaacag
      421 tcgacgaagc caagagtctg atcaatcttg ccaaggaagc gaatgtgata ggacaagtgg
      481 gacatgtaga acgattcaat cctgcatttt cggcatcagt tccctttttt caccaaccta
      541 tgtttatcga gtgtcaccgc ctggcagttt ggaatccgcg tggaaccgac gtttctgttg
      601 tgcttgatct gatgattcat gacatcgata tcatattaag cgtagtaaaa tccaacatta
      661 aacgaatcag cgcttcaggt gttgccattg tttcggattc accggatatt tcaaatgccc
      721 gcatcgagtt cgataatggc tgcgtagcta atctaacagc ttcccgaatt tcattgaaga
      781 acatgcgtaa aacccggttt ttccagagag atgcttacat cagtgtcgat tttctggaaa
      841 agaagacaga ggttgtgcgc atgaaacaaa ttgaaggtga accggatcct tttgcgttta
      901 tgattgatct gggtaacaat aaaggaaagc gcgaaatctt ttttgaaaaa cccgacgtaa
      961 aggaaaccaa tgcaataagg gatgagctac aggcttttgc acaggccatt ctttccggta
     1021 ataatccacc tgtaacttta caggatgggt tgaatgccct ggatgttgct tataagatca
     1081 ttggcaaaat ggaagttact tccgggattt cctgagacaa taaaaaaaat aactttgcgt
     1141 tcctttagta ctctctccga tggaacgtcg tctttatgta attattgccc tcattatcac
     1201 agggtttgtt tcttgccaaa aagatcaggg ggaaattccg tcctacattt atattccgga
     1261 tatccggctt accactacct ctgcacaggg ttcaacgtct gatgacatca ttgatgcctg
     1321 ggtatatgtg gatgataatc ccgttggttg cttcccttta cctgcccgtg tacccgttct
     1381 tgcggaggga aaccgcaaaa tttcggtgta cggcgggata aaagcagatg gcatcagtac
     1441 ccgccgccgc cgttatattt tttaccagcc ttttgtcacc aattccttta cacttcgtcg
     1501 tggaatggtg gatacattaa aaggtgtatt tgagcccgtt gtaagttatt acccttccaa
     1561 tcaaattacc atctggtacg aggattttac cgatccgttt gtgccattct ttcaggacgc
     1621 aataagtgat accacgatta accgaatcac agatccaggt gaagtttttg aaggtgcagg
     1681 ttgtggattg ctaacgctct ctgctacaca gaattttgtt caggtggcaa cttccgaaaa
     1741 ttttgatttg cccaaaggag gaattcctgt ctacctcgaa ttgaactaca aagccaacca
     1801 ggcatttgca gtcggattac gcagtattta cccgggtaat atcctcaatc aggaaaatac
     1861 cattatccgc gatacctacg atgataatgg tgtgttggta tggaaaaaga tttattgcga
     1921 attcacagaa ttggtgagct ccaatattaa tgcaatttct cacgaaattt tctttagggt
     1981 ttataaagac caaggtgtta gcgttcctca agtttacatc gacaacgtgc gactcatcta
     2041 tggcaattga tccgaaagca agacaggttt taaaatactt cttatcagat ctgataaccg
     2101 ctggccttgc atggacattg ttttttgttt ttcgtaaaac atggattgaa caaactgtaa
     2161 ttgagtttga tgaaaattat taccgcggac tcatcatcat tccgcttttc tgggtaactc
     2221 tttattacct cagtggctat tacagcaaca ttttccgcaa acaccgtatt caggatctcg
     2281 gctttacact cattcaaaca ttggttggtg ttctcttcct cttttttgtg gtgatcctcg
     2341 atgatgaagt gcgtacttac accagttatt attatgcctt ctttgcgtta ttcggggtac
     2401 attttctcct cacttttcta ccacggatat ttttcacctc acgattggtt cgtcgtattc
     2461 acaaacgcaa aatcggattc aataccttgc tcattggagg aaatgcgaat gcactgaata
     2521 tctatcggga gatcatggat atgaaacaat cgcccggata taaattcgtt ggttttgtaa
     2581 gtattaatgg ggttgatgat caattgagag aagaacattt gccctatctc ggaaaataca
     2641 aatccggaat tcaggaggta ttaaaaaatc acaaagtaga ggaggcaatt attgccatcg
     2701 aaagctctga acacgaaagc ctgaaaaaaa ttatcagtga tctggaaggt gatcaattgc
     2761 gcttgcacgt aattccggat atgtacgata ttttgaccgg atcggtacgg atgaccaaca
     2821 tttacggtac tccgctcatc gagatccgca gtcagctgat gccggcatgg caggcttccg
     2881 ttaaacgcat gatggatgtg ctgatttcct ccgtttcatt attgctttgc ctccctatgt
     2941 ttttggtgat tgccatcatc attaaattaa gcagtaaagg tcctgttttc ttcctgcagg
     3001 atagagttgg aatcaatggc cgacctttcc gcatcattaa attccgctcc atggtggtta
     3061 acgctgaaag taatggtccg caattaagca gcgaaaacga tccgcgtata acaggaatcg
     3121 gtcgtttttt acgcaagaca cgtcttgatg agtttccgca atttatcaat gttattttgg
     3181 gcgatatgtc gctggttggg cctcgtcccg aacgtcagtt ttatatcgat aaaatcatgg
     3241 agtttgcacc gcattaccgt tacctgaata aagttcgtcc gggtattact tcatgggggc
     3301 aggtaaaata tggttacgca gaaaatgttg aacagatgat tcaacgcatg aaatacgatc
     3361 tgatttatat cgagaatatg tccattgcac ttgatatcaa aatcatgttt tatacgcttg
     3421 ctataatctt taaaggaaaa ggaaaatgat tgttattcgt gtcttacaga tcgtcatcgc
     3481 tttgatgtct ctcttttttc tgtttatggc agtgtacgac cgctttttgg tcagtaatcc
     3541 tggtccggaa gaaaaatcgg gttttggact atacctttcc ttgtttggcg caggtgtagt
     3601 gatgagtttg ctactctggc gtttggcaaa gactgcggca aaacaccttt aattttcaga
     3661 ttgcttttgc aattcctttt caatcgaaga aaagtttcct caaattcgcc acatggaaat
     3721 tgttgtgatc gattataaag ccggcaacat ccgttcagtg ctcttcgcgt tggagcgaat
     3781 tggagcaaat gggattctca ccgatgatcc gcaacgtatt ttgaatgccg ataaagtcat
     3841 ttttcccgga gtaggagaag catcctcggc tatggaagcg ctaagaagtc gtggattgga
     3901 taagttgatt cccaccctca aacaaccgtt gcttggaatt tgccttggaa tgcaactcct
     3961 ttgttcgcac tctgaggagg ggaataccaa ttgtctgaat gtgtttcccg tgcaggtaaa
     4021 gcgatttccg gaaaagcgtg cgggtgtaga aaaagttcct catgtgggtt ggaacacggt
     4081 ttccggctta aagggagctt tgttcaatga tttgccagat cccgtttaca tgtactatgt
     4141 acatagcttt tatgctgaac aaagtgcctt taccacaggt gtttcccgac atatcatcga
     4201 ctattcagca tccttgcaaa aggataattt ctatgctgtt cagtaccatc ttgaaaaaag
     4261 cggggatgca ggggaacgat tgctaaggaa tttcattgca atttgataat tcccctttgc
     4321 gtcgttccga cctccaaatt gattaatatt gcagcctcaa aaacaagtaa acatgtcaaa
     4381 agtcctcatt atcggagccg gtggggttgg tcaggtggcc acctacaaat gtgcgatgaa
     4441 taccgatgta tttaccgaga tcgtgcttgc aagccgcacg aagtcgaaat gcgataaaat
     4501 cgccgaagac attaaagcca aattgggtgt caccatcaaa accgatcagg tggatgccga
     4561 taatgttgct gaattgatcg ccttgttcaa aaagcatcag ccaaaactgg ttgtaaacct
     4621 ggcactccct tatcaggatc ttaccattat ggatgcctgt cttgccacag gtacacacta
     4681 tatggatacc gccaattacg agccaaagga cgaagccaaa ttcgaataca aatggcagtg
     4741 ggcttaccag gagaaattta agcaggctgg attaacagca attctcggtt gtggattcga
     4801 tccgggcgtt acgggtgtgt atacttcata tgcagcgaaa caccattttg atgaaatcca
     4861 ttacctggat attgtggatt gcaatgcagg agatcatgga aaagcgttcg ctaccaactt
     4921 caatcccgaa atcaatatcc gcgaggttac ccaaaaggga aaatactggc aggatggtaa
     4981 gtgggtagaa acggagccac acgaaattca tatgccgctc aattatccga atattggtcc
     5041 gaaagaatct taccttattt atcacgaaga actggaatcg ctggtgaaga atttccccac
     5101 tatcaagcgt gcccgtttct ggatgacatt cggtcttgag tatttaacac acttacgcgt
     5161 aattcaaaat atcggtatgg cgcgtatcga tcctatcatt tacgaaggaa aagaaattgt
     5221 acctattcaa ttcctcaagg ccgttctccc caatccaggg gatcttggcg aaaattacaa
     5281 aggtgaaact tccattggtt gtcgcatccg cggaataaaa gatggtaagg agaaaaccta
     5341 ttacgtatat aacaactgtt cgcacgaagt tgcattcagc gaaaccggaa cacaggctgt
     5401 ttcctatact acaggtgttc ctgcaatgat cggtgcaatg atgttcttaa ccggacaatg
     5461 gaccggtgcc ggtgtatgga atgtggagca attcaatcct gatcctttca tggaacaact
     5521 caacaaacaa ggattgccat ggcatgaatt gcataatgtg gatcttgaat taaattaatt
     5581 gtatccgggg gatttatcct ccggatataa gagatacccc aatgattgat ctcaataaaa
     5641 tccccgcccc ctgttacgtt atcgaagaag aacttctgcg gaagaacctt actttgatac
     5701 gtgatgtatc acaacgtgca ggagtggaga tcatacttgc gtttaaggga tttgcaatgt
     5761 ggagtacatt ccccattgtg cgcgaatata tttccggagc aacagcgagc tcggtttacg
     5821 aagcacgatt gtgttttgaa gaaatgaaaa caaaagcaca ttcgtatgtt gttgccattt
     5881 ctcctaccga aatcgatgac ttacttgact attcttcggt attgaccttc aattctgttt
     5941 cgcaattcga acggtacaaa caaaaatgta tcgatcgcgg agtttccata ggaattcgtg
     6001 ttaacccgga gtattctgag gtaacaacag agctatacaa tccatgttcg cccaattcgc
     6061 gtttgggaat gaccagcgaa catttttctg atcgacttcc tgatggagtg gatggattgc
     6121 attttcatgc cttgtgtgag aatgattcct atacactgga aaaaacactg gtagccttcg
     6181 aagcaaaatt cggcaagctg attccccaat gcaaatgggt gaattttggt gggggtcacc
     6241 tcatgacgcg cgatggttat gatgttgaac atctcatttc tgttttgcaa tcctttaagt
     6301 cacgctatcc gggtgtaaaa gtgatcctcg aaccgggatc tgcatttgca tggcgaaccg
     6361 gttacctggt ttcaactgtt ctggatatcg ttgaaaacaa cggcgtaaaa accgccatgc
     6421 tcgatgtaag ctttactgca catatgcccg attgtctgga gatgccttat cgtccgcgca
     6481 tttggggagc aacagatcct gtgaaaggaa aaccaactta ccgcatggga ggcacaagtt
     6541 gtttgagtgg cgattatatg ttcgaatatt cgtttgagca ggaattgcag ccgggcaata
     6601 aaatagtttt tgatgatatg attcattaca ccatggtaaa aacgactatg ttcaacggtg
     6661 taaatcatcc atcttacggt atatggacaa gcaaaggaga atttcgtctg gtgcgcaaat
     6721 ttgcatatga agattataaa atgcgcttgt cgtaattgaa cgttcacccg aaccgaaaca
     6781 gacatacaat gattctgatt ccagctattg atatcattga tggaaaatgt gtccgattaa
     6841 ccaaaggtga ttattccacc cgaaaagttt acaatgagga ccctttggaa gtcgccatgc
     6901 aatttcaaga cgcgggatta actcatttgc atctggttga tctggatggc gcaaaagcct
     6961 cgcatatcgt aaactggaag gtgttggaga aaatcgctgc aagaaccaaa ctgcatatcg
     7021 attttggcgg aggattaaaa tcggataagg acttgcagat tgcgttcaat tcgggcgcag
     7081 aaaaaattac cggtggaagt attgcagtga aatcaccgga tgtttttaaa caatggatca
     7141 aagactacgg tacagaacga attatcctgg gtgcagatgt caaagaggag aaaatatcca
     7201 ttaccggttg gacagaagac acggatacat acgtatgtga ttttttaaat gattaccgtt
     7261 cctcatccgg aatagagtac gtgatttgta ccgacatcgc caaagacgga atgcttgagg
     7321 gtccttcgat ggaattgtat tcgaaaatac tgtcggaaat cccatccctg aaactgattg
     7381 ccagtggtgg tgtttcatct gtttcggatc tggaacaatt gaaggaaaag ggtttgtttg
     7441 ccgccattat cggaaaggca atttatgagg ggaaaatatc tttaggcgag cttgctaaaa
     7501 tggtaagtgc ctgatatgcg gttgcaggaa agtagattct aaaggctttt ttttcttgaa
     7561 taacagtacc agacttttgg ttggtacatt tctgatcagc tagggcaatt tcagtgaatt
     7621 ttgatggtgc gcatagtgta aatacctaaa tttaagcaat caattgttgg cagaaaccga
     7681 agtgtttatc ggataaaaaa attgatccat tttaaactat gctcactcac cgcattattc
     7741 catgtctgga tattaaggac ggtcgcaccg taaagggtgt gaattttgtg gatctgcgcg
     7801 atgccggaga tccggttgaa cttgcagtgc aatatgccgc acagggcgct gatgaattgg
     7861 tgtttttgga tattacagct accaatgaaa agcggagaac acttgctgag cttgtcagga
     7921 aaattgcgtc gctattaaat attccgttta ctgtaggtgg aggaatttct tctgttgagg
     7981 atgtttctat tctgcttgaa agtggtgcag ataaaatagc agtgaattca gcagcagtaa
     8041 aacgcccgga attgattact gaactggcat caaaattcgg aagtcagtgt gtggtggtcg
     8101 cagttgatgc aaaaaaagtg gaaggaaaat ggcttgtcca cctcaatgga ggtaagctgc
     8161 aaactgaaat cgaccttttt gattgggtaa aagaaattga acgcagaggt ggtggtgaga
     8221 ttttgttcac ctcaatggat cacgacggaa caaaaaatgg tttcgccaac gaagctttgg
     8281 aaaaaatcac atcactcgta aatattcctg ttattgcttc c
//
LOCUS       DAOMYG010000083         8311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2862_length_8311_cov_11.582364, whole genome shotgun sequence.
ACCESSION   DAOMYG010000083 DAOMYG010000000
VERSION     DAOMYG010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8311
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2862_length_8311_cov_11.582364"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="PK610_04025"
     CDS             <1..1124
                     /locus_tag="PK610_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRJ35126.1"
                     /translation="RVEFSEKIKLGFGFTGGATDRRFDPSNVVVVDNNDPTLVFSFPI
                     RKTYFDLSAGIYLDVDRFQFGVAVPQMLANTVRVSMDNSHILERHYMGHLKYNWLISP
                     TLDLSLIPLARVMYVPGAPFQYDVNITADLKKIGWITATYRSQYAVAANIGFRIKQDL
                     VFGYAYNFVMNTTNAYGPVNQEILVGYTFGVKSNDREKKLLEEAERENERLRQELLRK
                     QKEYDSLNTIKEGLDRRIDEQERYYNDTINKLNEELRKLREDMKNTQTKVTEVKNTND
                     GPPVKRTEGDHFVELDKSTESPKGYYVIDGAYGSMENAQSQYDRLKGQFPEARIIFNE
                     RNQLHYILLTYSEDKPKVFDTHRKAKSIGEEKAWVLEYR"
     gene            1378..6255
                     /locus_tag="PK610_04030"
     CDS             1378..6255
                     /locus_tag="PK610_04030"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HRJ35127.1"
                     /translation="MKRFLRIYLLVTILLATGSISAQNICFRANNDFTSANGTNGVVN
                     PRFLAKGDINGDGKLDIITSNGNQTGNPSSLSIFIGQGNGLFTYIDTITIGPNSSNTQ
                     AITLQDFNADGKLDIAVVKRNSLGGTFRLAVLQGNGVGDFTPWVEMTAPNFTNDLVTG
                     DFDADGMIDIVTISGASSNNISFFKNNGAGFNAQVISNPTFLGAGYNIKVGQVDHLTD
                     SHLDVVITRVSGSRVTLMRGNGTGSFTTGFNVVIGTDPRDLDLGDLNGDGNMDVAVAM
                     NGSDSVHVVFTNGMGAITSISRYYSGGRGPTSIVMFDYDGDGDKDIAVSNVLDHVIGL
                     LINDGSGNFTLQRHASFIDPSEILTGNFDSDLKEDIVFTGNSRRLMYMGNNSPNGFLS
                     YNNLYYPTINTQPNSSSIADFNGDGNTDLVTANSSSNNFSLFLGNGSGGYTFSSNTSA
                     SAMPLAIQSGDFNGDGFKDVLLVESSAQQLSLFPGDGSGNFGFPTSVGLPGTPISLSV
                     GDLDNDGDLDAVVTSNTSNAVYIYQNIAGVFSLAGTLSSGVNGPNNVSINNFNGDALP
                     DLAICNNGTSNVQIYTGTGSCNFTSGSSIVVSAGPRMVTSGDFNGDGNKDISVCYGSS
                     TISYANGTGTGTFSAAVNLTGITGAGQIVWLLSADLNADGFDDVVTCNNASSDVSALK
                     GGGLGLSFQQAFYSGYKPVHIAQGLVNNDLLPDIAICNSDATGASSVNVMLNRSAVIT
                     NSGAGSFCIGGGVTLTSTQAYSYQWSTVATTQSINVTTSNSYTVTTSNYNSSCNSISQ
                     PEVVTVNPLPTVSGVTGTTTICGSSSTSLTANSSASSPVFEWYDAVSGGTLLFTGATF
                     VTPVLGVNTSYWVQVTDGSSGCVSSPRFQVDVVIGDVLPPTINNCPSNITINVGAGQC
                     SATASWTAPSATDNCGSATINQTIGLPSGSVFPVGTHNIQYTAVDGSSNSSSCSFTVT
                     VVDNEAPVFTTCPSNISQNVAPASCNALVTWSAPTASDNCFATVTQTSGPASGSTLAV
                     GNYSVVYTATDAASNTSTCTFTITIVDNLSPTITGCPSNQTLSNTPGTCGRVVTWTAP
                     TATDNCAGATITQTAGPASGSTLPIGVTTITYTATDGASNTSTCSFTITVNDTQAPVI
                     AGCPSNQTQSNTPGTCGRVVSWTVPTVTDNCSATITQTAGPSPGNTFPVGVTNVTYTA
                     TDAAGNVSTCSFTVTINDTQGPTITGTPSNITLSATTGTCSATATWTPPTATDNCTVP
                     TIVQTSGPPSGSSFNAGTTPIVYTATDGAGNTSTVTFNVTVVDAAPPTFTNCPSNITI
                     CQGNPVTFPTPTATDGCDPSPTVTQIAGLPSGSVFPLGNTTVTFRATDASGNFTNCTF
                     TVTVNSSPTVSFVLADDNACVGDPLMSLTTGTPFGGIYSGTGVSGSAFNPATAGVGAH
                     TITYTVTNGNGCSAFATDVMTVFANPTVTFTMSPASICYNAAPVSVSGGSPAGGTYTG
                     SGIAAGVLTPTIGIVGTHTATYTVTNSNNCSASATSTYTIGCVDIDEQDIRSTFMSVY
                     PNPSNGNFMVEFAVPLNSPAMLNVFNAVGQWVTSIEVEGAQSAINISTLERGIYFITL
                     DIEGMRYVNKVIIER"
     gene            6396..7208
                     /gene="proC"
                     /locus_tag="PK610_04035"
     CDS             6396..7208
                     /gene="proC"
                     /locus_tag="PK610_04035"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRJ35128.1"
                     /translation="MKIAIAGFGNIGRIYARSIVKLNLTERKNVMILARSEQSKRTAL
                     DDGFECIHESPALIADAEIVVIAVKPQDFSVLASFLKQFLKEDQIIVSLMAGITIQQI
                     KENLAHSKIIRAMPNSPIALGMGMTGFCSSSDVMSKELRKAEQLLSTTGRTLYIDDEK
                     KMDAVTAVSGSGPAYFYFFVKSMIESAEKLGFESSVAALLVKQTMLGAFHVWNSSEDD
                     LDKLISTVASKGGTTQEALKVFEQRGFASTIESGLKKAEERGRELSGELNKH"
     gene            7243..>8311
                     /locus_tag="PK610_04040"
     CDS             7243..>8311
                     /locus_tag="PK610_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRJ35129.1"
                     /translation="MLRIKNKTEYELLYQQSVSDPARFWDAVASSFVWRKKWDTIIDG
                     DFSSADIRWFQGAKFNITENCLDRHLEVNGNKLAFIWEPNDPNEEERRFTYKELFNEV
                     CKCANMLRNNGVKKGDRVCIYLPMVPEAVISMLACARIGAIHSVVFGGFSAASLAGRM
                     QDCDNVVLITSDGAYRGDKEISLKKVADDALELCPSVKKVIVLQRTGAIVNMEEGRDQ
                     WWHDAMNGMSTQCEAEEMDAEDILFILYTSGSTGKPKGVVHTCGGYMVYTQYSFENVF
                     QYQPDDLFWCTADVGWITGHSYITYGPLLAGASQVIFEGVPTYPDAGRFWKIIDKYNV
                     SLFYTAPTAIRALEAAGTSFVE"
BASE COUNT         2388 a         1604 c         1826 g         2493 t
ORIGIN      
        1 accgcgttga attcagtgaa aaaataaaac ttggcttcgg atttacagga ggtgcaaccg
       61 acaggagatt tgatccgagc aatgttgttg ttgtcgacaa taatgatccg acattggttt
      121 tttcttttcc gataagaaaa acctattttg atttgtcggc aggaatttac ttggatgttg
      181 atcgcttcca atttggagtc gctgttcctc agatgttggc gaatacagtg cgtgtttcaa
      241 tggacaattc ccatattctg gagcgccatt atatggggca tttaaaatac aattggttaa
      301 tttctccaac tcttgatctt tcattgattc cattggcgag agtcatgtat gtgccaggtg
      361 caccttttca gtatgacgta aatattacgg ccgatctgaa aaaaattggt tggatcacgg
      421 cgacgtatag gagtcaatat gcagttgctg caaacattgg atttcgcata aagcaggatc
      481 ttgtatttgg gtatgcatac aattttgtga tgaacactac taatgcttac gggccggtga
      541 atcaggaaat cttagtaggg tatacatttg gtgttaagag caatgatcgt gagaagaaac
      601 tacttgaaga agcggaacgt gagaatgaac gcctgcgtca ggagttgttg cgtaaacaaa
      661 aagaatatga ctctttgaat acgatcaaag aaggtttaga tcgccgtata gacgaacagg
      721 agcgatatta taacgacaca ataaataagc tgaatgagga attacgcaag ctgcgtgagg
      781 acatgaagaa tactcagacc aaagtgactg aggtaaaaaa cacaaatgat ggtcctccag
      841 taaaacgaac cgaaggggat cattttgtag aattggataa atcaacggag tcgccgaaag
      901 gatattacgt tattgatggt gcttatggaa gcatggaaaa tgcacagtcg caatacgaca
      961 ggctgaaagg gcagttcccg gaggcaagga ttatctttaa tgagcgcaac cagttacatt
     1021 acatcttgtt gacttattct gaggataagc cgaaagtgtt tgatacccac cgtaaggcca
     1081 agtctatcgg cgaagaaaag gcctgggtgt tggaataccg ataaatccat ttatcagttt
     1141 agattcgatt tttgtcccaa aaccactgtt caaagcgata atccgaatta ttaagttttt
     1201 gtttttcaag gtcttatgtt tggaatccaa tccctaccca ataatggtaa gacctaaacc
     1261 tataacattc cattgttaat aaccgtaaga agcgctatct tagaaggctt gaatactaga
     1321 gaattcagtc tttcaggttg ttaataagta ctgaaattaa ttgttcaaac atacatcatg
     1381 aagcgatttt tacgcatcta cctacttgta acaattttat tggcgacagg ttccatttcg
     1441 gctcaaaaca tttgtttcag ggcaaataat gattttactt ctgcaaatgg aactaacgga
     1501 gttgtaaacc cgcgattcct tgcgaaaggt gatattaacg gagacggaaa actggacatt
     1561 attacttcca atggaaatca aacaggaaat ccatcttctc tttcaatttt tatcgggcaa
     1621 ggaaatggtc tgtttactta tatcgatacg attactattg gtccaaacag ttcaaatacg
     1681 caagcaataa ccttgcagga ttttaatgct gatggtaagc ttgatattgc tgttgtaaaa
     1741 agaaattctt taggtggcac atttaggctt gcggtgttgc aaggtaacgg agttggggat
     1801 tttacacctt gggtggaaat gacagcaccc aattttacga acgatttagt tacaggtgat
     1861 tttgacgcag acggcatgat tgatattgtt acaatatcag gcgcatcatc aaataatatt
     1921 tcctttttta agaataacgg tgcggggttt aatgcacagg taatttcgaa tcccactttt
     1981 cttggtgcgg gttacaacat caaagttgga caagtggatc accttactga cagccatctt
     2041 gatgttgtaa ttacacgtgt gtctggatcc cgagttacac tcatgcgagg aaatggaacg
     2101 ggatcgttta ccaccggttt taacgttgta atcggaacag atccgcgtga tttagatctg
     2161 ggcgatttaa atggtgatgg taatatggat gttgctgttg caatgaatgg atctgattct
     2221 gttcacgtgg tttttaccaa tggaatggga gcaattacat ccatttcgag atattattcc
     2281 ggtggtcgcg ggccaacttc aattgttatg tttgactatg atggtgatgg cgataaggat
     2341 attgctgtga gtaatgttct cgatcacgta atcggactat tgatcaatga tggctcaggt
     2401 aatttcacgc ttcagcgaca tgcaagtttt atcgatccat ctgagatttt aaccggaaat
     2461 tttgattccg atttaaaaga agacattgta ttcaccggaa attcgcgtcg actgatgtac
     2521 atgggaaaca attctccgaa tggctttttg tcttacaaca atctttatta cccaacaatt
     2581 aatactcagc caaatagttc ttcaatagca gatttcaatg gcgacggaaa tacggatctt
     2641 gtaactgcaa attcttccag caataatttt tcattgttct tgggcaatgg aagtggtgga
     2701 tatacttttt cttcaaatac atcggcatcc gcaatgcctt tggctattca gtcgggcgat
     2761 tttaatggag atgggtttaa agatgtattg cttgttgaat catctgctca acaactttca
     2821 ttattccctg gtgatggttc aggaaatttt ggttttccta cttctgttgg actacccgga
     2881 acacccatta gtttatcagt tggtgattta gacaatgatg gggatcttga tgcagttgtc
     2941 acatccaata cttccaatgc agtttatatt tatcagaata tcgcaggagt atttagccta
     3001 gccggaacac tttcatcggg agttaacggt ccgaataatg tttccataaa taatttcaat
     3061 ggtgatgcat tacctgatct cgctatttgt aacaatggaa cctccaatgt tcagatttac
     3121 accggaaccg gttcatgcaa ttttacttca ggatcttcta ttgttgtaag tgccggacca
     3181 cgaatggtta cttccggtga ttttaatggt gacggtaata aagatatttc tgtttgttat
     3241 ggttcaagta caatatccta tgcaaacgga acaggaacgg gtacattttc tgctgcagta
     3301 aatctaaccg gaattacagg tgcaggccag attgtttggt tgttatcagc tgatctgaat
     3361 gccgacggat ttgatgatgt tgttacttgc aataatgcat cttcagatgt atcggcatta
     3421 aaaggcggag gtctaggtct ttcctttcaa caggcatttt attccggcta caaaccggtt
     3481 cacattgcac agggtttggt aaacaatgat ctgttgccgg atattgccat ttgtaattct
     3541 gatgcgaccg gagcaagtag tgttaatgta atgcttaacc gctcagcagt aattacaaat
     3601 tccggtgccg gttcgttttg tattggaggt ggtgttacat tgacatccac acaggcatat
     3661 agttaccaat ggagcacagt tgcaaccact caatctatta acgttacaac atctaattct
     3721 tacacagtaa caacttctaa ttataacagt tcctgtaata gtatttctca accagaagtt
     3781 gtaacagtaa atcctttgcc aacggtttcc ggtgttactg gaactactac aatttgcggt
     3841 tcttcaagca ctagcttaac agcgaattca tcagcatcat ctccggtatt tgaatggtac
     3901 gatgcggttt ccgggggtac attgcttttt acaggcgcaa cttttgtaac ccctgtattg
     3961 ggtgtaaata cttcttattg ggtgcaggtc acagatggat cttccgggtg tgtttcttca
     4021 ccccgcttcc aggttgatgt tgtaattgga gatgtgcttc ctccaactat taataactgt
     4081 ccttccaata tcacgattaa tgttggggca ggtcaatgta gcgcaacagc gtcatggact
     4141 gcgccttctg caacagataa ttgtggttct gcaaccatca atcaaacaat cggacttccg
     4201 tccggtagtg tttttcctgt aggaacacat aatattcagt atactgcagt agatggttct
     4261 tccaactctt cgagttgttc gtttacagtt acagttgtag ataacgaagc ccctgtattt
     4321 acaacatgtc cttcaaatat ttctcagaac gtagctccgg cttcatgcaa tgcattagta
     4381 acctggtctg ctccaacagc atctgataat tgttttgcaa ctgttactca aacatcaggt
     4441 cctgcttccg gcagcacact ggctgtaggt aattattcgg ttgtatatac tgctactgat
     4501 gctgcatcca acacttcaac atgtacgttt acaattacaa tcgtcgataa tcttagtcca
     4561 acaattaccg gatgtccttc gaaccaaaca ctaagcaaca cgccaggaac atgtggtaga
     4621 gtagttacct ggaccgcacc aaccgcaaca gataattgtg ccggcgcaac cattacgcaa
     4681 accgcaggtc cggcaagtgg ctcaacgctt ccaataggtg taacaacgat aacctataca
     4741 gcaactgatg gcgcaagcaa tacttctaca tgctctttta caatcactgt aaatgatacg
     4801 caggcacctg ttattgcagg ttgtccttca aatcaaacac aaagcaacac acccggaaca
     4861 tgcggtagag ttgtttcatg gacagttcca acagttactg ataattgttc cgctacaatt
     4921 acccaaacag caggcccttc gccgggaaat acatttcctg ttggtgttac caatgtaacc
     4981 tacactgcaa ctgatgctgc cggtaacgtc tctacttgtt cgtttactgt caccatcaac
     5041 gatacgcaag gaccaaccat taccggaacg ccatctaata ttactttatc agcaacaaca
     5101 ggcacctgtt ccgcaactgc aacctggact cccccaacag caaccgataa ttgtactgtt
     5161 ccaaccatcg ttcaaacttc gggacctcct tcaggttcaa gttttaatgc gggtacaaca
     5221 ccgattgttt acacggcaac agatggtgca ggaaatacat ctacagttac attcaatgtt
     5281 acagtggtag atgcggcacc accaacattt acaaactgtc cttccaacat taccatttgt
     5341 cagggtaatc ctgttacgtt tcctacacca acggctacgg atggttgcga cccaagtcca
     5401 acagttacac agattgcagg tttgcccagc ggaagtgttt tcccattagg aaacaccact
     5461 gttacttttc gcgcaacaga tgcttcagga aactttacca attgtacatt cacagtaact
     5521 gttaacagtt cgccaacagt ttcatttgta cttgcagatg ataatgcctg tgtgggagat
     5581 ccgctaatgt cgctaactac aggtacacca tttggtggaa tttattcagg cactggagtt
     5641 tctggatctg catttaaccc ggcaacagca ggcgttggtg cacacacaat tacatacaca
     5701 gtaacaaatg gaaacggttg ctctgctttt gcgaccgatg tgatgacagt atttgcaaat
     5761 cctactgtta cgttcacaat gtctccggct tcaatttgtt ataacgctgc acctgtttcc
     5821 gtatcaggag gtagtccggc aggtggaacg tataccggct ccggaattgc cgctggtgta
     5881 ctaactccaa ctattggaat tgtgggaaca cacacagcaa cctacacagt aacaaactcg
     5941 aacaattgct ctgcatctgc taccagtact tatacaattg gttgtgtgga tattgatgaa
     6001 caggatattc gttcaacttt tatgtcggta tatccgaatc catcgaatgg gaatttcatg
     6061 gttgaatttg cagttccttt aaatagccct gcaatgttga atgtatttaa tgctgtcgga
     6121 caatgggtaa cttcaatcga agttgaaggg gcacaaagtg cgattaacat tagcactctt
     6181 gaacgtggaa tatatttcat caccttggat atcgaaggga tgcgttacgt gaacaaagtg
     6241 ataatagaac gataatagtg ttactattgc agaatgattg caccgaaaca aaatattaac
     6301 ccgaatattc cagagatgta agtcccggga aaattcatta ttgatattaa tctatcccgg
     6361 gtcaaaatga tccgggattt ttatttattg acgctatgaa aattgcaatt gctggtttcg
     6421 gaaacattgg caggatttat gcccgctcaa ttgtgaaatt gaatttaacg gaaagaaaaa
     6481 atgtgatgat tcttgcccga tccgaacaat cgaaaagaac agctttagat gatggatttg
     6541 aatgtattca cgaatcccct gctttaattg ctgatgcaga gattgttgtt atcgcagtta
     6601 aacctcagga tttttcagtg ctggcatcat tccttaagca gtttcttaag gaagatcaga
     6661 tcatagtttc gttaatggca ggcataacca ttcagcagat aaaggaaaat ctcgcgcact
     6721 caaaaatcat tagagcgatg ccgaattctc ccattgcatt gggaatgggt atgacaggtt
     6781 tttgctcgtc ttcagatgtg atgagcaaag aacttcgcaa ggcagaacaa ttgttatcta
     6841 caacaggacg aacattgtat attgatgatg aaaaaaagat ggatgctgta acagcagtta
     6901 gcggaagcgg cccggcatat ttttattttt ttgtaaaaag catgattgaa tctgctgaga
     6961 aacttggatt tgaatcttcg gttgcggcct tattggttaa acaaactatg cttggggctt
     7021 ttcatgtttg gaacagctct gaggatgatt tggataaatt gatttcaacg gttgcttcaa
     7081 agggaggaac cacgcaggaa gcgcttaaag tttttgagca acggggattt gcttctacca
     7141 tcgaatcagg gcttaaaaaa gcagaagaac gcggtaggga attatccggt gaattaaaca
     7201 aacactgaca gtctttttag tagattaggt aaaaatcata ttatgttgag gataaagaat
     7261 aaaacggaat atgagttatt gtaccaacaa tcggtaagcg atcctgctcg tttttgggat
     7321 gcagttgcgt catcttttgt atggcggaaa aagtgggata ccattattga tggtgatttt
     7381 tcttcagccg atattcggtg gtttcagggt gctaagttta atatcaccga aaactgtctt
     7441 gatcgccatt tggaagtaaa tggaaataaa ctggcattta tttgggagcc caacgacccg
     7501 aatgaagaag agcgaagatt tacttacaaa gagcttttta atgaggtttg taaatgtgca
     7561 aatatgttgc gcaataatgg cgtgaagaaa ggcgatcggg tttgcattta tttgcccatg
     7621 gttcctgaag cggtgatttc aatgctcgct tgtgccagaa taggagcaat acattccgtt
     7681 gtatttggtg ggttttcagc cgcatcttta gccgggagaa tgcaggattg tgacaatgtg
     7741 gttttgatta cttccgatgg tgcctaccgt ggcgataaag aaatttctct aaagaaagtt
     7801 gctgatgatg cattggaatt gtgtccctct gttaagaagg tgattgtatt gcagcgtacg
     7861 ggtgcaatag tgaacatgga agaaggcaga gatcagtggt ggcatgatgc aatgaatgga
     7921 atgagcacgc aatgcgaggc agaggaaatg gatgcagagg atatattgtt tattttgtat
     7981 acttcaggtt ccaccggtaa accaaaaggt gttgttcaca cttgtggcgg atatatggtg
     8041 tatacacagt attccttcga aaatgtgttt caatatcaac ctgatgactt gttctggtgc
     8101 accgcggatg ttggctggat aacaggtcat tcttacatta cttacgggcc gcttcttgcc
     8161 ggtgcttctc aggttatttt tgaaggtgtt ccaacttatc ctgatgccgg tagattttgg
     8221 aaaattattg ataagtacaa tgtgagttta ttttacactg caccaactgc gatcagggca
     8281 ttggaagcag cagggacatc ttttgttgaa c
//
LOCUS       DAOMYG010000084         8306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2867_length_8306_cov_9.939886, whole genome shotgun sequence.
ACCESSION   DAOMYG010000084 DAOMYG010000000
VERSION     DAOMYG010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8306
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2867_length_8306_cov_9.939886"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PK610_04045"
     CDS             <1..228
                     /locus_tag="PK610_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35130.1"
                     /translation="IEKRNDYFKAVHSKLREKGELIVVDFKKGDLPVGPPNAMKVGPN
                     TAKAELEEAGFTFVQMDDASLEYQYLLKMKK"
     gene            complement(331..744)
                     /locus_tag="PK610_04050"
     CDS             complement(331..744)
                     /locus_tag="PK610_04050"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HRJ35131.1"
                     /translation="MVQLRTRLLLLFLFFCCSTLSAQQTSFSFPDSVHLSLTEVQDSV
                     FVFYDTLSSNTENTKQRRPRRLVASLLCITLGPFGVHRLYLGTTPNVPVAYTLTLGGG
                     VGILPVVDLLLIVFSNDITPYLNNPNVFMWSEGEQ"
     gene            789..1007
                     /locus_tag="PK610_04055"
     CDS             789..1007
                     /locus_tag="PK610_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35132.1"
                     /translation="MKKILLYLSLTIALAITAFLVYRFIYTLNTGPSEEQLQEHKSHD
                     DEVVAPSKLDALEASFAEEDSTASDSLK"
     gene            1086..2156
                     /locus_tag="PK610_04060"
     CDS             1086..2156
                     /locus_tag="PK610_04060"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HRJ35133.1"
                     /translation="MKKSMLLLSMILVLVSACGSGSDYKSSDETSLETSGAAMNNDES
                     APSEIYYEAEESIKEDGDDENATQTLSAANAGRNLSYGYISPGAKTQPMVSERLFSSA
                     AVAFNVNDSLRPFIISADLRFRVANVEKSTYAIEDVVIANKGYVESTFLRSTVQSSRT
                     IPISRDSTLEQVDYVLENRMTLRVPSKHLDRVLRKIGAESEFFDHRNLTAENITFDLV
                     DKELERKRLAKYHSRLSKAIDDKGRKLESIESAEEALLRADERNDRNTVEKLRMLDKV
                     EYSTLEVFIYQNESVFTKVAANRKNIAQYEPSFWSKLGDSFIEGWEIVQDFILVLARA
                     WFLLLIAGFALWFLLKKYRTSK"
     gene            2175..2627
                     /locus_tag="PK610_04065"
     CDS             2175..2627
                     /locus_tag="PK610_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35134.1"
                     /translation="MKPGKNVRNIFFLLVLPGFLMLFSCTAPEHEISENKTSDSLGQL
                     LIDTALHFEEVKQLSLMLNKGHAGETQLSAIITARPSSAFDHYWIEIGESINGEFITY
                     FHFLFFPEAEEWKVYDEKTDAVMDRSQWKTERMNAPLLQHKTPPPDRE"
     gene            complement(3010..3444)
                     /locus_tag="PK610_04070"
     CDS             complement(3010..3444)
                     /locus_tag="PK610_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35135.1"
                     /translation="MNTLLLLTALLLNPSVDTNVVLHYYHGGGMYYGSQDYYIRYDSV
                     VVEDMDHGLVLRRAYAMNDQLRKEVKEAIGTYRLNTIRTLPCGIIYDKATTSVKLTRG
                     TDVLAYVSSGATTQVVEADRDRFHAFVDWMTNFIHRISAIGK"
     gene            complement(3548..4930)
                     /locus_tag="PK610_04075"
     CDS             complement(3548..4930)
                     /locus_tag="PK610_04075"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRJ35136.1"
                     /translation="MKRASIYVMIGSPFAIAGVIYLTEYGWFANMPVAIPLLVTLLAF
                     ASLFLSFAFFYMHSPRRKKAMIVLGVIGLLITMLGYIFKLQHWPGANVQTIVGVLIIC
                     FSYSPLRFYEKYLRWKGLSLSHRDALYLSISELLGSVFFFLGILFKFMHWPYANFMMT
                     SGALVLGASVLGWNRKFKQEVVQRKQMQDDLHSANDLLAEKNKEITDSINYAKKIQDA
                     YLPPMRVFEHYFQEGFILFQPKDIVSGDFYWFFKEGFAENKASDWVYLAVADCTGHGV
                     PGAIMSVICCNALNDVAVTRAIQSPEKILDETRQLVVRTLKSENGSGRQDGMDISLCR
                     FNYKSGELQFAGAHNPAIVVKAGGAEVIELGADKQPIGFFERAFPFTLKEYQLQKGDT
                     LYLFTDGYADQFGGPKGKKLKFANLQKLLQSMHNIPMQQQKQQLTDAFENWRGNLEQI
                     DDVCVIGVRL"
     gene            5133..>8306
                     /locus_tag="PK610_04080"
     CDS             5133..>8306
                     /locus_tag="PK610_04080"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35137.1"
                     /translation="MKKQFLLLIIAVHLFSFPYYGNSQALQSAFPQLNAGGQINAIAE
                     DSVNNILYLAGNFTHVNGIARRNLAAVDLATNTVLSSFNPISTMNGSILCLARYGTRL
                     YVGGEFTSVNGSGAVPYFCRIDLNASGNNGTLNTVYQPGGASPVQDMVLDGSTLYLAG
                     QGVLIDHLPSGSLRLAIAAIDAINGNLLSYDPASRPANGLLTFTSCTIVKLLLAPTRI
                     YACGQQFGGNGNEGIIALDKSNGDAIPSFNPVFNFAKVIDAERRNGKIYILNSKIWPL
                     GYSLMEFDENTNVMTPGAFMITGGTPESIGIYQDELFIAGSFTNVLSQSRNGFAAVDL
                     NTLSCTPFSPNPIGVLDHPRAIQISRNNLYISDAALTTISSQSRIGLAKYCMKPHPAN
                     AISISDASICPNQQGIIATVPAARYATTYTWNYTGTGINLTGTGTFNTLNASSNPSSG
                     NLIVTPQSYCAINGSSSQLPLTIEQAPFVYAGNDTGLTCIQTSVLLSGNSNAQSANYV
                     WTNPQLIQTFNQNISIQSAGEYHLSVTDGITGCTAKDTVLVIADTSRPAINLPVGNFL
                     LSCSDTTLFLSGNVFPATASSYWRLQGGGLYSDPFLCQSPGQYYLVGIDNTNGCRDSN
                     FIFVQQNIQAPQLFLLHPNQSFILDTLNCRDTVLNISGMAIPGTTDLEWSDGSNTLNG
                     NTISITNGGLWALSGIRQDNGCQSVINFYIYEWKTPPQNQITSDTLLLNCSFPEKWTD
                     NFTSTINTSIEIKKDTSWLPYPQLIDTPGIIHYSIRDLRNECVLRDSFIVIHSNTFSF
                     AGSTDSVVCMNTEFLPIPDPINAQQNLYYSSSGIPPSSFVPVCTIVFDTTVVIHVTDS
                     TCSGDYVINLLLPPPRADSLFSVSSCIDSSGSVSVISYGGAPPFLYSINQSPFQLSNS
                     FTSLPYGEYTLEIKDNYQCRSIYEITLESTSALPVNRFMVDTRASREDTIVIVNTTLP
                     QPDSINWAFSDSLQFIDQTGSSLILKITDTLPHLIRSTAFFLGCESSSERLIQWREKI
                     MISDSIYISILVFPNPSSGIVTIRISSN"
BASE COUNT         2480 a         1907 c         1674 g         2245 t
ORIGIN      
        1 attgaaaaac gaaacgatta ttttaaagca gtacactcca aactccgcga aaagggagaa
       61 ttgattgttg tcgattttaa aaaaggtgat ttgccggtgg ggccgcccaa tgcgatgaag
      121 gtaggtccaa acaccgcaaa agcagaactg gaagaggcag gatttacatt tgtgcaaatg
      181 gatgatgcct cgttggagta tcagtatttg ctgaagatga aaaaataatt ggcatcacct
      241 gcaattaaga ctttttaaga attccgttta cttgtttcca aaactaactt gggttacctt
      301 tcccccacac tcggtattta ttagcagaac ctactgctct ccttcgctcc acatgaatac
      361 gttggggttg ttgagatagg gtgtgatgtc gttggagaaa acgatgagca gcagatcgac
      421 cacaggaaga attcctacgc ctccgcccag tgtgagggtg taggcaacag gaacgtttgg
      481 tgtagtgccc aaatacaaac ggtgcacgcc aaaaggaccc aatgtaatgc acaacagaga
      541 agctacaagt cgtcgcggac ggcgttgttt tgtattttct gtattagaag aaagggtgtc
      601 gtaaaaaaca aaaacactat cttgaacttc agttaaagaa aggtgtacag aatcaggaaa
      661 tgaaaaagaa gtctgttgcg cggaaagagt gctgcagcaa aaaaataaaa aaagtaaaag
      721 aagacgcgta cgcaactgaa ccatgatcca aaaatacgaa tcaaaagcat agacctaatc
      781 aaaacgaaat gaaaaagatc ttgttatacc tatctcttac cattgcgctg gcaataacgg
      841 cctttcttgt ttatcgtttt atctatacct tgaataccgg tccgagcgaa gaacaattgc
      901 aggaacataa atcgcacgac gatgaagtgg tagcgccttc caaactcgat gcactcgaag
      961 ccagctttgc agaagaagat tccactgcaa gtgatagttt aaaataaacc aattccattt
     1021 cagcacaata ccatcgggag aagttcttcc gcaggaaaaa gtatatccaa ccaaaaccat
     1081 ttgccatgaa aaagtcaatg ttattactga gcatgattct cgttttagtt tccgcctgcg
     1141 gctccggcag cgattacaaa agttcagatg aaacgagcct ggaaacatcc ggcgccgcta
     1201 tgaataacga cgagtccgcc ccaagcgaaa tttattacga agcggaggaa tcaataaaag
     1261 aagacggtga tgatgagaat gctacacaaa cattatccgc agccaatgcc gggagaaatc
     1321 tttcatacgg atatatatct ccaggtgcaa aaacacagcc aatggtgagc gaacgcttat
     1381 tctcttctgc agctgttgca tttaatgtaa acgattcttt gcgtccattt atcatttcgg
     1441 ccgaccttcg ttttcgtgta gccaatgttg agaaaagcac atacgccatc gaagatgtag
     1501 tgattgccaa taagggttat gtagaatcca ctttcctgcg cagcactgta caaagttcgc
     1561 gtaccattcc catttcgcgg gattcaacac ttgagcaggt ggattatgta ctcgaaaacc
     1621 gaatgacatt gcgtgtgccg tccaaacacc tcgatcgtgt attgcggaaa atcggtgcgg
     1681 aatccgaatt ttttgatcac cgtaatctca ccgcagaaaa catcaccttc gacctggttg
     1741 ataaagaact cgagcgcaaa cgtcttgcaa aataccattc gcgactttca aaagccatcg
     1801 atgataaagg aagaaaactg gaaagcattg aaagcgcaga agaagcactt ttacgcgcgg
     1861 atgaacgtaa cgaccgcaac actgttgaaa aacttcgtat gctggataag gttgaatatt
     1921 ctactcttga agttttcatt tatcagaacg aaagtgtgtt taccaaagtt gctgccaatc
     1981 gtaaaaacat tgcgcaatac gaaccttcct tctggagcaa actgggcgat tcctttatcg
     2041 aaggttggga aattgtgcag gatttcattc ttgtgcttgc ccgcgcatgg ttcctgttgc
     2101 tcattgcagg atttgcactt tggttcctgc taaaaaagta ccgcacatct aaataatttt
     2161 tcgatcttta gtgcatgaaa cccggtaaga acgtcaggaa catctttttt ctcctcgttc
     2221 ttcctggttt tcttatgctc ttttcctgca ccgcaccgga gcatgaaata agcgaaaaca
     2281 aaacaagcga cagcctcggg caactattga ttgataccgc actccatttt gaagaggtga
     2341 aacaattatc gctgatgctg aataaaggtc atgcaggaga aacccaactg agcgccatca
     2401 ttaccgcacg accatcaagc gcatttgatc attactggat tgaaatcggc gaatccatca
     2461 acggagaatt cattacgtat ttccattttc tattttttcc cgaagcagag gaatggaaag
     2521 tgtacgacga aaaaacagat gctgttatgg atcgttctca atggaaaacg gaacgcatga
     2581 acgctcctct actgcagcac aaaacaccac cgccggatag ggaatgatga ttggtgaatg
     2641 gtgatcccga cgctaaaggt cgggacaggc cggtgaacag tgaacggtga acagtaatca
     2701 gtgaacagtg aacggttctg aagtagaagg gtgctctact tattaaattt agattgaata
     2761 tcaattttcg ggtgtgcact tttcccaatg gtagcgtttt tcaaaacaaa ttcttcttgt
     2821 ccgccgactt gtccactgac ttgtccactg aaatgaaacg aaagtggaag tggaacaaag
     2881 ggtgtaggag gatgcggtaa aaccaccaag aacgtattcc aatgaaaaat ctattcctgc
     2941 agatctccat tcgttactcc ctactattaa cttttaacta ttaactccta actaaagact
     3001 cataacttct tactttccta ttgcactaat gcggtgaatg aaattcgtca tccaatctac
     3061 aaaagcatga aagcgatctc tatcggcttc tactacttgt gttgttgctc cggaagaaac
     3121 atatgccagg acatctgtcc cccgcgttaa ttttaccgat gtggttgcct tatcgtaaat
     3181 aataccgcag ggaagtgtac ggatagtatt caaacgataa gtgccaatgg cctctttcac
     3241 ttctttgcgc aactgatcat tcattgcata cgcacgccgc agtaccaatc catggtccat
     3301 gtcctccacc actacactat cgtaacggat ataataatcc tgcgagccat aatacatgcc
     3361 accaccgtgg taatagtgca gcacaacatt ggtatccacc gaaggattca acaataaagc
     3421 ggtaagaaga agaagcgtgt tcataatatc agattttata gctgtacacg tgccggagaa
     3481 aaaggttcag ggggggtgaa aggtgaacag taaattcgaa attcgttctc gttcccagtc
     3541 ccgttctcta caaacggact cctataacgc acacatcatc gatttgttcc aggttccctc
     3601 tccagttttc aaacgcatca gtgagttgtt gtttttgttg ctgcatggga atgttgtgca
     3661 tcgattgcaa tagtttttgc agattggcga attttagttt cttgcctttg ggtccgccaa
     3721 actgatcggc gtaaccgtcg gtgaaaagat agagtgtatc gcctttttgc aattggtatt
     3781 ctttcagtgt aaatggaaat gcacgctcga aaaatccgat cggctgttta tcggcaccca
     3841 gttcaatcac ttcggctcct cctgccttca caacaatagc aggattgtgt gcgccggcaa
     3901 attgtagttc tccgcttttg taattgaatc tgcataagga aatatccatc ccgtcttgtc
     3961 tgcctgatcc gttttcgctt tttaatgtgc gtacaacgag ctgacgtgtt tcatcgagaa
     4021 tcttttcggg cgattgtatt gcgcgggtta cagcaacatc attgagtgca ttgcagcaaa
     4081 tcacactcat gatcgcaccc ggaacaccat gacctgtaca atcggctaca gcgagatata
     4141 cccagtcgga tgctttgttt tctgcgaatc cttctttgaa aaaccagtaa aaatcgccgc
     4201 ttacaatatc cttgggctga aacaaaataa aaccctcctg aaaataatgt tcaaaaacgc
     4261 gcataggagg aagataggcg tcctgaattt ttttagcgta attgatgcta tccgtgatct
     4321 ccttgttttt ttccgcgagc aaatcattag ccgaatgaag atcgtcctgc atttgtttgc
     4381 gttgcaccac ttcctgttta aacttccggt tccatcccaa tacagaagcc cccagaacca
     4441 aagcacccga tgtcatcatg aaattagcat aaggccagtg catgaacttg aagaggattc
     4501 ccaaaaagaa aaaaacagag cctagcaatt cggaaatact caggtacaat gcatcgcggt
     4561 gtgaaaggga aagtcctttc cagcgaaggt acttttcgta aaaccgcaag ggagaataac
     4621 tgaaacagat aatcaacaca ccaactatag tttgcacatt tgctcccggc caatgttgaa
     4681 gtttgaaaat ataccccaac atggtaatca gcaatccgat cacgcccaat acaatcattg
     4741 cctttttacg ccgaggcgag tgcatgtaga aaaaggcaaa actcaaaaac aaggaagcga
     4801 atgccaacag tgtaaccaac aatggaatcg ctaccggcat gtttgcaaac caaccgtatt
     4861 cagttaaata gatcacacct gcaatggcaa atggagaccc gatcatcacg tatatggagg
     4921 cgcgtttcat tatgttaaat gtagggatat ttagtgaaca gtgatcctga cgctccctga
     4981 tgatctttgg cggtcccttt acaactccca aaatcccctt ccctccatct gttcatcaac
     5041 ccatccatcc ctccccaccg aactgttcac ttaacaagta agttggtaca gaatcgcttg
     5101 tatgccgttt ttcgtgcggc tagttttgaa gcatgaaaaa acaattcctt ctgttgatca
     5161 tcgcggtaca ccttttcagt ttcccctatt acggaaattc acaggctttg caaagcgctt
     5221 ttccgcagtt aaatgccggc ggacaaatca acgctattgc cgaggacagt gtaaacaaca
     5281 ttttatacct cgccggaaat tttacgcatg taaacggaat cgcgcgccgg aatcttgctg
     5341 cagtggatct tgccaccaat accgtactca gttccttcaa tcccatcagc accatgaacg
     5401 gtagcatact ttgcctggcg cgttacggca cacgtttgta tgttggcggg gaatttactt
     5461 cggtgaacgg ctccggtgct gttccgtatt tctgtcgcat cgatcttaac gcaagcggca
     5521 acaacggaac actcaacacc gtttaccagc cgggcggcgc ttctcctgtt caggatatgg
     5581 ttttagatgg cagcaccctc tatcttgccg gtcaaggtgt gctcatcgat catttaccca
     5641 gcgggtcgct gcgtttggcg attgccgcta ttgatgctat caacggaaat ctgctgagct
     5701 acgatcctgc cagtcgtcct gctaacggac tcctaacctt tacttcctgt accattgtaa
     5761 aactattgct ggctccaacg cgcatctatg catgcggaca acaattcgga ggaaacggca
     5821 acgaaggcat cattgccctg gataaatcaa acggagatgc aattccctct ttcaatcctg
     5881 tgttcaattt tgcaaaagtg atcgatgcgg aaaggagaaa cgggaagatc tatattctga
     5941 attcaaagat ttggcctctg ggatattcat taatggaatt tgatgaaaat acaaatgtga
     6001 tgacacccgg tgcatttatg attacaggag ggactccgga atcgatcgga atatatcagg
     6061 atgagttgtt cattgccgga tcttttacaa atgtcctaag ccaatcacga aacggttttg
     6121 ctgctgttga tctgaatacc ctttcttgca cgccattttc tccgaaccca atcggtgttc
     6181 ttgatcaccc gagagccatc cagatatcga gaaataatct gtacatcagt gatgctgctt
     6241 taaccacaat ttcttctcaa agccgaatcg gccttgctaa atactgcatg aaaccacatc
     6301 ctgcaaatgc catttcaatt agtgatgcaa gcatctgtcc aaatcaacaa ggcataatag
     6361 caacagtacc ggctgcgcgc tatgcaacta cctacacttg gaattataca gggacaggaa
     6421 ttaatttaac cggaacaggg acattcaaca cacttaatgc ttcgtcaaac ccgtcctccg
     6481 gaaatttaat tgtaacacca caatcctatt gcgcaataaa cggttcatcc tcacaactac
     6541 cactaacaat tgaacaagct ccttttgttt atgcaggaaa tgataccggt ttaacatgta
     6601 tccaaacttc agttttactt tcaggtaaca gtaatgcaca atcggcaaat tacgtttgga
     6661 ccaatccgca acttatacaa acattcaatc aaaacatttc catacaatca gcaggagaat
     6721 atcatctatc ggttacagat ggaattaccg gctgcacggc aaaggacacg gtattggtta
     6781 tcgcagatac gagcaggcct gcaatcaatc ttcctgttgg aaattttctt cttagctgca
     6841 gtgatacaac actttttctc tccggaaatg tatttcctgc aacagcatct tcctattgga
     6901 gattgcaagg tggcgggctg tactctgatc catttctttg tcagtcaccc ggtcaatact
     6961 atttggtagg aattgataat acgaatggtt gcagggacag caatttcatt tttgttcaac
     7021 aaaacataca agctccacaa ctttttttac tccaccctaa tcagagtttt attctggaca
     7081 ccctcaactg cagagatacc gttttaaaca tctccggcat ggcaattcca ggtacaactg
     7141 atttggaatg gtctgacggt tcaaatacac tcaacggaaa taccatttcc attacaaatg
     7201 gcggattatg ggccttgtcg ggaattcgtc aggataatgg atgtcaatca gtaataaatt
     7261 tttacatcta cgaatggaaa acaccaccac aaaatcaaat tacttcagac acactgttac
     7321 tgaattgctc gtttccggaa aaatggacag acaactttac aagtaccatc aacacaagca
     7381 ttgaaataaa aaaagacact tcctggttgc cgtatccgca attaattgat acgcccggaa
     7441 ttattcacta ctcaattcgt gatttaagaa atgagtgcgt actaagggat agttttattg
     7501 tgatccatag caatacattt tcatttgccg gatcaacaga ttcagtggta tgcatgaata
     7561 cagaattcct acctattccg gatccgataa atgcccaaca aaacctctac tactcatcat
     7621 cagggatacc accaagctca tttgttccgg tctgcaccat tgtgtttgat acaactgtgg
     7681 taattcacgt tacagattca acatgtagtg gggactatgt aataaatcta ttgcttccac
     7741 cgccaagagc tgattcacta ttttcagtct catcctgcat agacagctcc ggaagtgttt
     7801 cggtaatttc ctatggtggt gcaccgccat ttttgtattc gatcaatcag tccccatttc
     7861 agttgtccaa cagttttaca tcactacctt atggagaata tacattggaa ataaaagaca
     7921 actatcagtg tcgatccata tacgaaatta ctcttgaatc aacttctgca cttccggtaa
     7981 acagatttat ggtagacaca agagcatcgc gggaagacac tattgtcatt gtaaatacca
     8041 ccttacccca accggattca ataaattggg cattttctga ttcacttcaa ttcattgacc
     8101 aaacaggtag ctctctaata cttaaaataa ccgacaccct tccacacctc ataagaagca
     8161 ccgccttctt tcttggttgt gaatcatcat cagagcgatt gatccaatgg cgtgaaaaaa
     8221 taatgataag tgactccatt tacatttcaa tacttgtctt tcccaaccca tcatccggaa
     8281 tagtaaccat tagaatttca tccaat
//
LOCUS       DAOMYG010000085         8257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2891_length_8257_cov_8.311022, whole genome shotgun sequence.
ACCESSION   DAOMYG010000085 DAOMYG010000000
VERSION     DAOMYG010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8257
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2891_length_8257_cov_8.311022"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PK610_04085"
     CDS             complement(<1..860)
                     /locus_tag="PK610_04085"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRJ35138.1"
                     /translation="MKKLMIAGLLAGCLNVSLEAQRNLPIGLTPEEEQLMMSGSFQFG
                     STQRGVTAVPSFPVRTMAQWEEVQSLVLTWTSYPAILAQIADAAQEEAQVIIHCSDSN
                     AVKTYLTNAGVPLYNIRYIETSFNSIWIRDYGAHTIYKNEVDSLLLLEWIYNRPRPAD
                     DAMPNVYAAFKGIGIHSTTQSPNDLVNTGGNWMVDGFGTAFASKLILDENEPGNPYGV
                     SAKTEAEIDTIMKRYMGISRYIKMETLPYDDIHHIDMHMKLLNEETILVGQFPAGISD
                     GPQIEANLLYV"
     gene            1047..1382
                     /locus_tag="PK610_04090"
     CDS             1047..1382
                     /locus_tag="PK610_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRJ35139.1"
                     /translation="MDIENAKAQMKKGVLELCILSILNRQESYPGDIIEEMKASKIIL
                     VEGTLYPLLTRLKNAGILTYRWEESTSGPPRKYYKLTPEGEEFLQELLGAWQELVSAV
                     KTVSVKKLN"
     gene            1390..2952
                     /locus_tag="PK610_04095"
     CDS             1390..2952
                     /locus_tag="PK610_04095"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRJ35140.1"
                     /translation="MNKTITANVGGFVFNIEEQAYESLSRYLAAVRRSLGADECVDEI
                     MQDIEHRIAELFREFLQKEHKEVIGQQNVAEVIAIMGEPAAFAQEESRTENEQASTTW
                     SDEQTDKQFFRDSDNSMIGGVCSGISAYFGWDPLFLRILFVILMIGFGSGLLLYVILW
                     IIIPEAKTTSEKLRMRGKKVDVETIKQRFNDFKKDVEHLSTPEGKRKMRRASSRMADA
                     MSESAKNFYDIFGRALGGVLLILGLLFLIWLIKATISSSFMISLSEQGITAVNFEEFG
                     NAFFGTSTRTNLVYFAVIGLILMPILGLLFAAVRLLFRIPFKTKAIGAVMGLITSACF
                     ITLTVIGIQTGTDFAKENSQVTTTIIQTNSDTLYLDSSSDIYFSDHFKHHDDTFFELL
                     KFEEKEIVYGYPLVDVEQSPDSNFRVNINREARGSNQTEAISRAKNIDYQFAVQDSLV
                     TFNPWFSSPRADLIRGQQIKVNIQIPNGKTIHLSQRSDRIIYDIQNEEDLSDEDMVGF
                     YWTMKEKNLHRK"
     gene            complement(3040..3651)
                     /locus_tag="PK610_04100"
     CDS             complement(3040..3651)
                     /locus_tag="PK610_04100"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ35141.1"
                     /translation="MKKLLFSLGVPALFSTVQAQTTHTVTAVGFTYSPDTVECIVGDD
                     VLFSLSSLHNAVEVSQATFLANGSTSNGGFTVPFGGGSINMSVAGTYYFACANHSLSG
                     MKGVIIVSNATSVKEENAAFSVNVFPTKMEDILNIRMELPVAGMPVIKIFNMLGAEVL
                     SALTQTLNAGAAQMQLNVASLTAGTYFVRIEFAGLVKTMKVIR"
     gene            3774..6212
                     /locus_tag="PK610_04105"
     CDS             3774..6212
                     /locus_tag="PK610_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRJ35142.1"
                     /translation="MLKEKESVKNELTFEQFRDQVIEDFKLANLSREASLLGRKEVLT
                     GKAKFGIFGDGKELAQIALAKQFQNGDFRSGYYRDQTFMMAIGELNVQQYFAALYAHT
                     DTEHEPASGGRQMGGHYSTRSLNNDGTWKNLTEKKNSSADISPTAGQMPRLLGLAQAS
                     KLFRNNENLHSFTQFSNKGNEIAIGTIGDASTSEGLFWETMNAAGVLQVPMLMNVWDD
                     GYGISVPKKFQTTKESISEALKGFQRNEKQKGYEIFNVKGWDYAALCDTYARAAAICR
                     KEHIPVLVHVEEVTQPQGHSTSGSHERYKSKEILDWTRDFDCVKKFREWILEYSQQSG
                     KVIATEAELDEIQNNAKKDVRAAQKKAWEEFCAPLKNELNQVLALLDELAQESKQSAF
                     IQKQAESLRTAIDPIRRDILTAARRSLHYAAGENSNAVTKLSAWIRQSISDNYGRYSS
                     HLYSESAESALKINEVAPRYSASSEQVDARLVLRENFDHILGTNPLVLIFGEDSGNIG
                     DVNQGLEGMQAKYSDLRVTDTGIREATILGQGIGLSMRGLRPIAEIQYLDYLLYALQI
                     MSDDLATVQYRTKGGQKAPVIIRTRGHRLEGIWHSGSPMGAIINAVRGVYVCVPRNMT
                     QAAGFYNTLLASDDCGLIIEPLNGYRKKEVMPDNLGEFRTPLGIPEVVQEGTDLTIVS
                     YGATFNLCVEAAQQLAEKGISAELIDVRTLLPFDLNHIIADSLAKTNRLLVVDEDVPG
                     GASAYILDKVMVEQNGYFHLDSAPVTLAAKEHRPAYGSDGDYFSKPSVEDIFETAYHI
                     MHDADPASFPALYR"
     gene            6272..6598
                     /locus_tag="PK610_04110"
     CDS             6272..6598
                     /locus_tag="PK610_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ35143.1"
                     /translation="MIKRILLLALVFQVSILKADPPPPVQVICEQVIAGLRQQLESTN
                     IVLTSNVDKTLKLRISKNYEIFDSKGKLVKKGSGKEIDVSDLASGRYTIKFDKDSNQI
                     EYFTRN"
     gene            6677..7486
                     /gene="murQ"
                     /locus_tag="PK610_04115"
     CDS             6677..7486
                     /gene="murQ"
                     /locus_tag="PK610_04115"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRJ35144.1"
                     /translation="MFRKTTESESHYRDLEKMSTADLLSGINNEDKKVALAVEKTIPG
                     IAALVDEIVARMQKGGRLFYMGAGTSGRLGIVDASECPPTFGVPHGWVIGLIAGGDAA
                     IRKAVEFAEDNTEQGWKDLLEHAIGEEDTVIGIAASGTTPYVIGALKTCREKGILTAC
                     ITCNPGSPVTEVCDHPLVAEVGPEFVTGSTRMKSGTAQKLMLNMISTSVMIRLGRVKG
                     NRMVDMQLSNVKLIDRGTRMVMEALNIPYDEAKALLNQYGNVRKAIEQGNR"
BASE COUNT         2480 a         1846 c         1810 g         2121 t
ORIGIN      
        1 acataaagca agttcgcttc aatttgcgga ccatcagaaa tacctgcagg aaattgacca
       61 acaagaatgg tttcctcatt gagtaatttc atatgcatat cgatgtggtg aatatcatca
      121 tatggcagtg tttccatttt aatgtagcgc gaaattccca tgtaacgttt catgatggta
      181 tctatctctg cttccgtttt tgcgcttaca ccgtaaggat ttccgggttc attttcatcg
      241 agaataagtt tggatgcaaa tgcagttccg aatccgtcta ccatccagtt tcctccggta
      301 tttacgaggt cgtttggcga ttgggtggtg gaatgaattc cgattccctt aaatgcagca
      361 tatacattcg gcattgcatc atcggcagga cgcggacggt tatagatcca ttccagcaat
      421 aaaagtgaat ccacttcatt cttataaatt gtatgtgctc cgtaatcgcg gatccagatg
      481 gaattgaatg aagtttcgat ataacgaatg ttatacaagg gaacaccggc attggtgagg
      541 taggttttta ccgcattgga atcgctgcaa tgaataataa cctgcgcttc ttcctgagca
      601 gcatctgcaa tctgagccag aatggctgga tagcttgtcc aggttaacac cagcgattgt
      661 acttcctccc actgtgccat tgtgcgcacc ggaaatgatg gaaccgcagt aacaccgcgc
      721 tgggttgatc caaattgaaa gcttccactc atcatgagtt gctcttcctc aggagtgagt
      781 ccaatgggaa gatttcgttg tgcctccagc gacacattta aacatccggc caaaagtccg
      841 gcaatcatca gttttttcat agcattcagt aaccctacaa tgtaaaaaga atcgcttcag
      901 tgccctaacc acaggataaa tgaagaattc taaaaaaata tttcattgaa tttcaacgat
      961 ttaagtattt tttcagtact atgtattgca aaatactgtt ttatgtacat atctttgcaa
     1021 tgtcaagaaa caacatcaca ggaattatgg atatagaaaa tgcaaaagcg caaatgaaaa
     1081 aaggggtcct ggagctttgt atactctcca tcctcaacag acaggagtcg taccccggag
     1141 atatcattga agaaatgaag gcatctaaga tcatattggt tgaaggaaca ttgtatcctt
     1201 tactcacccg attaaaaaat gccggaatcc tcacctaccg ctgggaagaa tcgacatccg
     1261 gtccgccgcg taaatattac aaactcactc ccgaaggcga agaatttctt caggaattac
     1321 ttggagcctg gcaggaactt gtaagtgctg taaaaactgt aagtgtaaaa aaattgaact
     1381 gaacccacca tgaataaaac tatcaccgcc aacgttggag gatttgtctt caacatcgaa
     1441 gaacaggctt atgaaagtct gagtcgctac ctcgccgctg ttcgtcgctc tctgggagct
     1501 gatgaatgtg tcgacgaaat catgcaggat atcgagcacc gcattgctga attgtttcgc
     1561 gagtttctgc aaaaggaaca caaagaagtt atcggtcagc aaaatgttgc agaggtaatc
     1621 gccataatgg gagaacccgc tgcatttgca caggaagaat cgcgtactga aaatgaacag
     1681 gcatcgacta cctggagcga tgaacaaacc gacaaacaat ttttccgcga ttcggataat
     1741 tccatgattg gcggtgtttg ttcgggaatc tctgcttact tcggttggga tcctctcttc
     1801 cttcgtatac tgtttgttat actgatgatc ggattcggat caggattact gctctacgtt
     1861 attttgtgga tcatcattcc cgaagcgaaa accaccagcg aaaaactgcg tatgcgcgga
     1921 aagaaagtgg atgtggaaac cattaaacaa cgcttcaacg acttcaaaaa agatgttgaa
     1981 cacctcagta ctcccgaagg aaaacgcaaa atgagaagag cctcctcacg aatggcagat
     2041 gcaatgagtg aatcggcgaa aaacttctac gatattttcg gacgcgcatt aggcggtgtg
     2101 ttgctgatac tcggactcct ctttttgatc tggctcatca aggcaacgat ttccagcagc
     2161 tttatgatca gcttatccga acagggaatt accgctgtga atttcgaaga attcggtaat
     2221 gctttctttg gaacaagcac ccgcaccaat cttgtctatt ttgcagtaat cggattgata
     2281 ctaatgccta ttttgggatt gctctttgca gcagtgcgtt tattgttccg tattccattt
     2341 aaaacaaaag ccatcggtgc ggttatggga ctcatcacct cggcatgttt tataacgctt
     2401 acggtaatcg gaattcaaac tggaaccgat tttgcaaaag aaaattcgca ggtaactacc
     2461 accattattc aaacgaatag cgatacgctt tacctcgaca gttcctccga tatttatttt
     2521 tccgatcatt tcaagcatca cgacgacacg ttttttgaat tgctgaaatt tgaggaaaaa
     2581 gaaattgttt acggataccc gctggtagac gttgaacaat cacccgactc caatttccgt
     2641 gtgaacatca accgcgaagc aagaggttcc aatcagaccg aagccatcag cagagcaaaa
     2701 aacatcgact accaatttgc agtacaagat tcattggtca ctttcaaccc ctggttcagt
     2761 tctccgaggg ccgatctgat ccgtggtcag caaataaagg tcaacatcca aattcccaat
     2821 ggtaagacca tccatctcag tcaacgcagc gacaggatca tttacgatat tcaaaacgaa
     2881 gaagatttgt cggacgaaga catggttggt ttttactgga ccatgaaaga gaaaaatcta
     2941 caccgcaaat aacaagagag gcagcagcaa gagagaggca gcagcaacaa aaaaaggggg
     3001 atgatgatca tccccctttt cggtttatta aaaatggtat cagcgaatca ccttcattgt
     3061 ttttaccaga ccggcaaatt caatacgcac aaaataagtt cctgcagtta gtgaagcaac
     3121 attcaattgc atctgtgcag cacctgcgtt aagggtttgt gttagagcag aaagcacttc
     3181 agcacccagc atattgaaaa ttttgatcac aggcataccc gcaaccggaa gttccatacg
     3241 aatgttcagg atatcctcca ttttcgttgg aaacacattc acagaaaaag cagcattttc
     3301 ttcttttaca gaagtcgcat tggagacaat gatcacgcct ttcattccgg ataaggaatg
     3361 gtttgcgcag gcaaaataat aggttcctgc aaccgacatg ttaatgctgc cgccgccaaa
     3421 gggaacggta aatcctccgt tagaggtaga accgttggca agaaatgtag cctgactcac
     3481 ttccacagca ttgtgcaggc tggaaagaga aaacaataca tcatcaccaa caatacattc
     3541 aacggtatcc ggagaatagg tgaatcccac tgcagttaca gtatgcgtgg tttgtgcctg
     3601 aacagttgaa aaaagtgccg gaacaccaag ggagaataga agttttttca tcaacaattg
     3661 gatttatttg cttcaaatat aacaaaatag acgaaagtaa caagcttgaa attctccttt
     3721 tcggggggct tttttcctta tctttatacc ccgaaaacaa caatctattc gagatgctga
     3781 aagaaaaaga gagtgtgaaa aacgaactga cttttgagca gttccgcgat caggtcattg
     3841 aagactttaa acttgccaac cttagccgcg aagcttcttt gctgggaaga aaggaagtcc
     3901 ttaccggtaa agccaaattc ggaatattcg gcgatggtaa ggaattagcg cagatcgccc
     3961 tggccaaaca atttcaaaac ggagatttcc gttccggata ttaccgcgac caaaccttta
     4021 tgatggccat cggtgagctg aacgtgcaac agtatttcgc agcattgtac gcccacaccg
     4081 ataccgaaca cgaacccgca tcaggcggac ggcaaatggg cggtcactac tctacccgtt
     4141 cgctgaacaa tgatggtacc tggaaaaacc tgaccgaaaa gaaaaattca tcggcagata
     4201 tttctcctac tgcaggacaa atgccgcgtc tgctgggatt ggcacaggct tccaaactgt
     4261 tccgtaacaa tgaaaatctt cattcgttca cgcaattctc caataaagga aacgaaatag
     4321 ccatcggaac cattggtgat gcatccactt ccgaaggtct gttctgggaa accatgaatg
     4381 cagcgggtgt gttgcaggtg cctatgctga tgaatgtatg ggacgacgga tacggaattt
     4441 ctgttccgaa aaaattccag accaccaaag aaagcatctc cgaagcactg aaaggattcc
     4501 agcgcaacga gaaacaaaaa ggatatgaaa tttttaatgt aaaaggttgg gattatgctg
     4561 cgctgtgcga tacctatgcg cgtgctgctg ccatctgcag aaaagaacat attccggtat
     4621 tggttcatgt ggaagaagta acgcaaccac aaggacactc aacttccggc tcgcatgagc
     4681 ggtacaagtc gaaagaaatt ctcgactgga cccgcgattt cgattgcgta aagaaattcc
     4741 gtgaatggat cctcgaatac agtcagcaat caggaaaagt gattgccacc gaagcggaac
     4801 tcgacgaaat tcaaaacaat gcaaaaaagg acgttcgtgc cgctcagaaa aaagcatggg
     4861 aagaattttg tgcaccactc aaaaatgaat tgaaccaggt tttggcgctc ctggatgaac
     4921 ttgcacagga atccaaacaa tctgcattca tccaaaaaca ggcagaatca ttgcgcacag
     4981 ccatcgatcc gattcgtcgc gatattctta ccgccgcacg tcgttcgctt cactacgcag
     5041 caggggaaaa cagcaatgca gtaaccaaac tatctgcctg gatccgtcaa tccatttccg
     5101 ataattacgg tcgctacagt tcgcacctct acagtgaatc tgcagaaagt gcgctgaaaa
     5161 taaatgaagt tgcaccgcgt tattctgcct cttccgaaca ggttgatgca cgtttggtat
     5221 tgcgcgaaaa cttcgatcac attctcggaa ccaatccgct ggtactgatc ttcggagaag
     5281 attccggtaa tattggtgat gtgaaccagg gactcgaagg aatgcaggca aaatattctg
     5341 atctgcgtgt aaccgacacc ggtattcgcg aagccacgat acttggtcag ggaattggat
     5401 tatccatgcg cggattacgt ccgatcgcag aaatacaata cctcgattat ctcctctacg
     5461 ctttacagat catgagcgat gatctggcca ctgtacaata ccgcaccaaa ggcggacaaa
     5521 aggcaccggt catcattcgc acacgcggtc atcgtctgga aggtatctgg cattccggtt
     5581 ctccaatggg agccatcatc aatgccgtac gcggagtgta tgtatgcgta ccgcgtaaca
     5641 tgacgcaagc agcaggattc tacaacacac tgctggcatc cgacgattgc ggattgatca
     5701 ttgaacctct gaacggttac cgcaaaaaag aagtgatgcc tgataacctc ggagaattcc
     5761 gcacaccatt gggtattccc gaagttgtgc aggaaggaac tgacctcacc atcgtatcct
     5821 acggagcaac ctttaacctt tgcgttgaag ctgcacagca actcgcagaa aaaggaatca
     5881 gcgctgaact cattgatgtg cgcaccttgc ttcccttcga cctgaaccat atcattgccg
     5941 atagtcttgc aaaaaccaat cgcttactgg ttgtcgacga agatgtgccc ggtggtgctt
     6001 ctgcctacat actcgataag gtgatggttg agcaaaacgg ctatttccat ctcgattccg
     6061 cacctgtaac acttgctgca aaagaacatc gtcctgccta cggttccgac ggtgattatt
     6121 tttccaagcc aagcgtggag gacattttcg aaacggccta tcacatcatg catgatgctg
     6181 atcctgcaag ctttcctgcc ctttaccgct gattttttca gcaggaattt caggcatgaa
     6241 tattgcaaga acaacatgat cgcttaaaaa aatgataaaa cggattctgc ttctcgcttt
     6301 ggtttttcag gtcagcatcc tgaaagccga tccacctcct ccggtgcagg tgatctgcga
     6361 acaggtgatc gccggtttac gtcagcaatt ggaaagcacc aatattgtgc ttacatccaa
     6421 tgtcgataaa accctcaagc tgcgcatcag caagaattac gagatcttcg acagcaaagg
     6481 aaaactggtg aaaaaaggaa gcggaaaaga aatcgatgtt tccgatctcg catccggacg
     6541 atacaccatc aagttcgata aagactccaa ccagatcgaa tacttcacca ggaattaatc
     6601 cgatctgcaa agaatagaag agcgtcaaac ggcgctcttt ttttattctc tatattcggc
     6661 ttgtaaatcc ttgttcatgt tccgcaaaac caccgaaagc gaatcgcatt accgtgatct
     6721 cgaaaagatg tcgacagcag acctgctttc cggcatcaac aacgaagaca aaaaagttgc
     6781 gctggccgtt gaaaaaacca ttcccggaat tgcagcgctg gtggatgaaa ttgttgcacg
     6841 tatgcaaaaa ggcggccgct tgttttatat gggtgcggga accagcggac gattgggcat
     6901 tgtggacgca tctgaatgtc cgcctacgtt tggtgtaccg cacggatggg tgatcggact
     6961 gattgccggt ggagatgccg ccattcgcaa agcggtggaa tttgcagaag acaatacgga
     7021 acaaggttgg aaggatttat tggaacacgc cataggagaa gaagataccg taattggcat
     7081 tgctgcatcg ggaacgacgc cttacgttat tggcgcattg aaaacctgca gggaaaaagg
     7141 aatcctcacc gcatgcatca cctgcaatcc gggtagtccg gttactgaag tctgcgatca
     7201 tccattggtg gcagaagtgg gacccgaatt tgtaacagga agcacccgaa tgaaatcggg
     7261 aacagcgcag aagctgatgc tgaatatgat ctccacatca gtgatgattc gtttgggacg
     7321 cgtaaaagga aaccgcatgg tggatatgca actgagcaat gtaaaactca tcgaccgcgg
     7381 cacacgtatg gtgatggagg ctttaaacat tccgtacgac gaagccaagg cattgctgaa
     7441 ccaatatggc aacgtgcgga aggcaatcga acaaggcaat aggtaatccg aatgaaattc
     7501 cggagaaaat agatcaattg aaaaagcaat tactgttcct tcaccgcttt ctaccgttcc
     7561 ttcagtttct gttatgcaac tcctttcaac attcattgat ttacattatt tttttctaat
     7621 tttaattcat gctctatcgc aattctttca caagaaagtt gatttcaatt tcactcattt
     7681 tgtgtgtatg ctggattgtt tttggagttc aatacaaatt tctggcggat caaaaagcca
     7741 ttcaaaaaga aataaaaaaa agaatcaagc aaggtgttcc tgaatccgaa ttgtttgaat
     7801 tcgatttaaa taaaatcagt aaggatccgg gtttttcttg ggtaaaatca aacgagttca
     7861 ttttgggtaa tgaaatgttt gacattgtcc atcgaaaagg taacctggct ttttgtatca
     7921 atgacaaaca ggaagctcag ttgtttcaat atttagatga aatgaccaga aatgcctgga
     7981 acgcaaaaca cgattttaaa cgatttgttt ccattcaatt tttttttacc ggaaatgaat
     8041 ttacatccga tcccatttcc gttattcgat actttaaaat tccggaaaaa gactatgggc
     8101 ttcttcccgg attctgttta gagaaagaat atcctccgcc cttttcgggg acctgctgat
     8161 aattctttgt tgggctacaa tactacgccc atcaatgctt tatcacactg aaggggattg
     8221 tttcaatttt gaaaatcaaa atccttcaag gatatac
//
LOCUS       DAOMYG010000086         8225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2908_length_8225_cov_10.063525, whole genome shotgun sequence.
ACCESSION   DAOMYG010000086 DAOMYG010000000
VERSION     DAOMYG010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8225
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2908_length_8225_cov_10.063525"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1251
                     /gene="aroB"
                     /locus_tag="PK610_04120"
     CDS             172..1251
                     /gene="aroB"
                     /locus_tag="PK610_04120"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRJ35145.1"
                     /translation="MNKQTTHHHPVEIGSLAFSGLEELLVSSGLKEARKFILVDENSL
                     QYCFPVLVSQIEALRQAELIEIESGEHNKNITICTHIWEALSDMQTDRQALFINLGGG
                     VISDMGGFIASTYKRGIRFINIPTTLLSQVDASVGAKVGIDLNGLKNQIGLFAEPLAV
                     FIDPVFLSTLPTRELLSGFAEVIKHALIADKSYWELILNSHPLGNADWEPIIQKSVAI
                     KQSIVEADPTEKGFRKVLNFGHTIGHAVESLSLEGGRTPLTHGESVAIGMICESYLSE
                     RKRKMNKEELSSISTLITSLYEHRVFEDMDTHRLIELMKNDKKNKDDSISFTLLDGIG
                     KPVHDLSCTADEISDSFRYYHSLYR"
     gene            1251..2495
                     /gene="aroA"
                     /locus_tag="PK610_04125"
     CDS             1251..2495
                     /gene="aroA"
                     /locus_tag="PK610_04125"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRJ35146.1"
                     /translation="MNIQVTAPYKQICSRLALTGSKSESNRVLIIAALSKEKFTIHDL
                     ASARDTQTLIDILVQLPDVHEGVEQVFDVGPAGTAMRFLAGLLSISPGRFLLTGSERM
                     KNRPIGILVDALRSIGAEISYAGKEGFPPLRIRGKKLSGGVVNLNPGVSSQFVSSLML
                     IGPVLPDGLNISFTKEPVSRPYLNMTMELMRYFGAEINWEGNEIRIASSPYQSRDFCV
                     EADWSAASYWFEVVALAKEADLFLGGLHSESLQGDRVVREIFEMLGVQSHYEEGGIRL
                     IKNQDAIVPEFLNFDFSDCPDIAQTLACTCAGLGIGGHFTGLKTLRIKETDRLTALQN
                     ELAKFGVRTEIPSDDEMILFSGELKTPSVPVNTYEDHRIAMAFAPLALVAGPVIIEER
                     SVVDKSYPAFWTDLVQVGFELN"
     gene            2518..5277
                     /locus_tag="PK610_04130"
     CDS             2518..5277
                     /locus_tag="PK610_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ35147.1"
                     /translation="MNNRIRFFIVFAGFILLQLLPTDRIRFTGDAMGGNDPSDPDGRL
                     AWELERHADPSTGLIPAGIRQRELAFAATLPKNFQRSINFSLIGPKELGGRTRALAFD
                     VRDQNILLAGGVTSGIWRSTDAGAHFERVSDIGDLNSISCIVQDKRAGHEDTWYAGTG
                     EYYGVVSGTEFTSQMSGNGIYKSTDNGLSWSLLSSTTSNTPQTMYRNGDMDFVWKIVV
                     DPTAAGNSTVLAAVFNGIFRSTDGGTSWQPVLGFDSTTTANSDYTDIIVTPGGVFYAA
                     LSSDGPTKGIWRSVDGVNWNSIQSGFPTTYRRIAMAYNPSNENEVMCVMESPSSGTNG
                     HSLFKYTYVSGNGSGSGGVWENRSANLPAGSCTGFFDFDFGYFQTQGGYDMHINWHPS
                     NSNIVFLGGTNLYRSNDQFTSPVYKWVGGYRCNQSNLAQYVYPNHHPDLHGMVFLDSL
                     RAMTMSDGGVHLSTNVLADSVHWISLNNGYVTSQFYTVSIEEGNAYNDITLGGAQDNG
                     IWFTKFRHIDSLWKWVYRGDGSYTGIPNGRPHWIFSIQQGKMYKMIMTDDGDTILGTR
                     IDPVGIAATNNFINCFVLDPSNTNRLYMITRNAKIWRNDSLGTIPVIGDIYNRITHGW
                     TEITQSTLTLSQGNITVCAISPANPGRVYYGSNKSHVLKLDQAAGPTPVKTAISASIF
                     PPNSYVSSIGTNEFDGDELLVTFSNYGIRSVFHTPDAGVTWDDVSGNLEEFPDGSGNG
                     PAVYWSLIYPSAPEKIYLVGTSVGLYSTNQLNGINTIWQQEAPGVIGNSIVNMIKARA
                     SDGKMVVATHGNGIFSAQLTPLFLSSPELKSAELHFATYPNPFAEAITIDLYLPSEGK
                     LDVRVFDISGREIRVIYSGTSGSGEQRLIWQREDANGREIASGTYVLSVAHNGKRLTR
                     KLIVR"
     gene            5325..5744
                     /locus_tag="PK610_04135"
     CDS             5325..5744
                     /locus_tag="PK610_04135"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRJ35148.1"
                     /translation="MRKSYITRRENFNAAHKLWHDEWSEEKNFEVFGKCANKNWHGHN
                     YNLFVTVKGVPDPVTGFIVDLKALSVLIRNEVIEKLDHRNLNVDVDFMKGIKPSTENL
                     AIKIWEILEEPVRTLGGELHNIRLWETENNYAEYFGE"
     gene            5753..6388
                     /gene="folE"
                     /locus_tag="PK610_04140"
     CDS             5753..6388
                     /gene="folE"
                     /locus_tag="PK610_04140"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRJ35149.1"
                     /translation="MANPTDIISENSLDGYERVEQYNEGTLKEISDAYSKIIPALGED
                     PKREGLLKTPERVAKALQFLTHGYDLNPKEILQSAMFAEEYRQMVIVKDIEVYSMCEH
                     HMLPFFGKAHIAYIPNGHIVGLSKIPRVVDAFARRLQVQERLTTEIRDCIQETLSPLG
                     VAVVIEASHMCMQMRGVQKQNSVTTTSAFTGEFLKDPTRKEFITLIGSHLK"
     gene            6427..7170
                     /locus_tag="PK610_04145"
     CDS             6427..7170
                     /locus_tag="PK610_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HRJ35150.1"
                     /translation="MSNNMFNTSTNVGSGGSSSFSGVIPRTFVANVFSYMLAALAITG
                     VVAYAIGSNLELFVRLFISETGRPNPLFWIVSFAPLGLVLLMNFAFNRLSAPVLLGVF
                     ILYAGMMGLSLSSIFIMYTTGSIAAVFFITAGTFGVMAFLGYTTKTDLTKFGSILMMG
                     LIGIIIASIVNIFMQSSGLEWLISIIGVLVFTGLTAYDMQKIKEMGEQVGSGHELASK
                     MAIMAGLTLYLDFINLFIMLLRLLGNRNE"
     gene            7260..8138
                     /gene="fabD"
                     /locus_tag="PK610_04150"
     CDS             7260..8138
                     /gene="fabD"
                     /locus_tag="PK610_04150"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRJ35151.1"
                     /translation="MKKAYVFPGQGSQFSGMGKDLYEQSPLAKEMFEKANSILGFRIT
                     DIMFSGSDEELKQTKVTQPAIFLHSVILAATLGESFKPDMVAGHSLGEFSALVANKTL
                     SFEDALVLVSKRALAMQKACEAEPSTMAAVLGLDDAKVEAICTATEGVVVAANYNCPG
                     QLVISGAVDAVNRACEALKAAGAKRALLLPVGGAFHSPLMEPARVELEAAINATQFHT
                     PVCPVYQNVTASAVIDPEAIKKNLVAQLTAPVAWTQSVRQMTADGATQYVEVGPGKVL
                     QGLVKKISPEVEVASA"
BASE COUNT         2304 a         1579 c         1891 g         2451 t
ORIGIN      
        1 gatcatggcg gtgtattatg ctgcaaatat atctgtaaaa gcaaccggta agccgttttt
       61 ttgggtcaaa atagaggcaa ctattgatct ctaccgggag atgatttgag caaaggtatt
      121 ttgctgtttt tcatgtgcga tagtctcatt ttggctatct tgcacatagc aatgaacaaa
      181 caaacaacac accatcatcc ggtagagatt ggttctctgg ccttttcagg actcgaagaa
      241 ttacttgttt cctccggact aaaagaggcc aggaagttca ttcttgtgga tgaaaattcg
      301 ctgcaatatt gttttccggt cttggtcagt caaatagagg cattgcgtca ggctgaatta
      361 attgagattg aaagcggaga gcataacaaa aacatcacca tctgcacaca tatttgggaa
      421 gcgctttccg atatgcaaac cgatcgccag gcattgttta tcaatctagg tgggggagtg
      481 atcagcgata tgggaggttt tattgcatca acctacaaac gaggcattcg ttttatcaat
      541 atccctacca cattacttag tcaggtggat gcttctgttg gagcaaaagt cgggatcgat
      601 ctgaatggat taaaaaatca aattggattg tttgccgaac ctcttgctgt atttatcgat
      661 cctgtatttt tatctacact tccgacgcgt gaattgcttt ctggatttgc tgaagtgatt
      721 aaacatgcat tgatcgccga taaatcgtat tgggagctga tccttaattc gcacccgttg
      781 ggaaatgcgg attgggaacc tatcattcaa aagtctgttg cgataaaaca atcaattgtt
      841 gaagctgacc caacagaaaa gggatttcga aaagtgttga atttcggaca tacaattggt
      901 catgctgttg agagtttatc gctggagggt ggacgcactc cgcttaccca cggcgaatcc
      961 gttgcaatag gcatgatctg tgaaagttat ttgtctgaac gcaaacgtaa aatgaacaag
     1021 gaagaactat cgtcgatcag cactttgatc acttcattgt atgagcatcg tgtgtttgag
     1081 gatatggata ctcatcgtct gattgaatta atgaaaaatg ataaaaagaa caaagacgac
     1141 agtatcagtt tcaccttgct ggatggtatt ggaaaacctg ttcatgacct ttcctgtacc
     1201 gcagatgaaa taagtgattc atttcgctat tatcatagtt tataccgtta atgaatatac
     1261 aggtaacagc tccttacaaa caaatttgtt ccagacttgc tctcacaggt tcaaaaagtg
     1321 aaagcaaccg ggtgctgatt attgctgctt tatcaaaaga gaaatttaca atacacgatc
     1381 ttgcaagtgc gcgggatact caaacattga ttgatatttt agttcagctc cccgatgttc
     1441 atgagggtgt tgaacaggtt tttgatgttg gtccggccgg aacagccatg cggtttctcg
     1501 cgggattgtt atccataagt ccgggtagat ttttattaac cggaagtgag cgaatgaaaa
     1561 atcgtcctat tggaatactt gtggatgcac ttcgttccat cggcgcagag atcagttatg
     1621 caggtaaaga aggatttcct cctttgagaa ttcggggtaa aaaattgagc ggaggagttg
     1681 tgaacctgaa tccaggtgtg agcagtcagt ttgtttcatc gctgatgctc attggacctg
     1741 tattgccgga tggattgaat atttcattta caaaagaacc tgtttcgcga ccctatctga
     1801 acatgaccat ggagctaatg cggtattttg gtgctgagat taactgggag ggaaatgaaa
     1861 tccgtatagc atcatctcca tatcagtcgc gtgatttttg tgttgaagct gattggagtg
     1921 cggcttcgta ttggtttgaa gtggttgctc tcgcaaaaga agccgatttg tttttgggcg
     1981 gacttcattc cgaaagttta cagggcgatc gtgttgttcg tgaaattttt gaaatgcttg
     2041 gagtacaatc acactatgaa gaaggtggaa tccgcctcat caagaatcag gatgcaattg
     2101 ttcctgagtt tctcaacttt gatttttctg attgtcctga tattgcgcaa acgcttgcat
     2161 gtacctgtgc cggccttggg attggcggac attttaccgg attaaaaact ttacgaataa
     2221 aagagacaga tcgtctcact gcattgcaaa atgaattggc aaaatttggt gtgcgtacag
     2281 aaatcccttc tgacgatgaa atgattttgt tttcaggaga actaaaaacg ccctcagttc
     2341 cagttaatac ctacgaagat catcgaattg caatggcatt tgctcccttg gcattggtag
     2401 ccggtccggt aataattgaa gagcggagtg ttgtagataa gtcctatccc gcgttttgga
     2461 ccgatcttgt tcaggttgga tttgaattga attaatagtt aagaaataaa tgtttgtatg
     2521 aataaccgta ttcgcttttt cattgttttt gcaggattca ttcttcttca gctccttcca
     2581 acagaccgta ttcgttttac cggagatgcg atgggtggta atgatccttc cgatcctgat
     2641 ggacgacttg catgggaact tgagcggcat gctgatcctt caaccggatt gatcccggca
     2701 ggtatacggc aacgagaact tgcttttgca gcaactcttc caaagaattt tcaacgttca
     2761 ataaattttt ctttgattgg accaaaagaa ttgggtggac gaacacgcgc tcttgcattt
     2821 gatgttcgtg atcagaatat tttgctggcc ggtggtgtta caagcggaat ttggagaagt
     2881 acagatgcag gtgcacattt tgagcgtgtt tctgatatcg gtgatctcaa tagtatttca
     2941 tgtatagttc aggataaaag agcaggacat gaagatacat ggtatgccgg tacaggcgaa
     3001 tattacggag tagtcagtgg aactgaattc acctcacaaa tgagtggaaa cggaatttat
     3061 aaatcaaccg acaatggttt gagttggtct ttacttagtt ctacaacttc aaatacaccg
     3121 caaaccatgt acagaaatgg cgatatggat tttgtttgga aaattgttgt tgatccaaca
     3181 gcagcaggaa attctactgt actggcagct gtatttaacg gtatctttag aagtactgat
     3241 ggtggtacaa gctggcaacc tgtgcttggg tttgattcaa ccaccactgc aaattctgat
     3301 tatacagata tcattgttac gccaggcgga gttttttatg cagcattatc aagcgatggt
     3361 ccaactaaag ggatttggcg ttcggtagat ggagtgaact ggaattccat tcaatccggt
     3421 tttccgacaa cctacagaag aattgcaatg gcttataatc cttccaatga aaatgaagtg
     3481 atgtgtgtaa tggaatctcc aagttccggt acaaatggac attcattgtt taaatacacg
     3541 tatgtctccg gaaacggatc aggaagcggt ggtgtttggg aaaatcgttc agctaattta
     3601 cctgccggaa gttgtacagg tttttttgat ttcgattttg ggtattttca aacacaaggc
     3661 ggatacgaca tgcatatcaa ttggcatccg tctaattcga atattgtttt tcttggcggg
     3721 acaaacctct accgatcaaa tgatcagttt acaagtccgg tttacaaatg ggtaggtggt
     3781 taccggtgca atcaatccaa tcttgcacag tatgtttatc ccaatcacca ccccgatctt
     3841 catggaatgg tgtttctgga ttccttgcgt gcaatgacca tgtcggatgg tggagtacac
     3901 ctgagtacga atgtgcttgc cgattctgtt cactggattt cattaaataa cggttatgtc
     3961 acttcacagt tttatactgt atctatcgag gagggaaatg cctataacga tatcacacta
     4021 ggtggagccc aggataatgg tatttggttt acgaaattca ggcatatcga ttcactctgg
     4081 aaatgggtgt accgcggaga tggttcgtat accggaattc caaacggacg accacattgg
     4141 atcttttcaa ttcaacaggg aaaaatgtat aaaatgatca tgaccgatga tggtgatacc
     4201 attttgggaa cacgcattga tcctgttgga attgctgcta ccaataattt cataaattgt
     4261 tttgtccttg acccttccaa tactaatcgt ctctatatga ttacgcgcaa tgcaaaaatc
     4321 tggagaaatg attcactcgg aacaattccg gttattggtg atatatacaa tcgaattaca
     4381 catggctgga ccgagatcac tcaaagtaca ttgaccttat cacagggaaa cataacggtt
     4441 tgtgcaattt ctcctgctaa tccgggtcga gtatattacg gttcaaacaa aagccatgtt
     4501 ttgaaattgg atcaggctgc agggcccact cctgtgaaaa cagcaatttc tgcttctatc
     4561 tttcctccca attcctatgt aagttctatc ggaaccaatg aatttgacgg agatgaatta
     4621 ttagtaacat tttccaatta tggaattcga agtgtctttc atactccgga tgcgggcgta
     4681 acctgggatg atgtttccgg taatcttgaa gaatttccgg atggaagcgg taatggtcct
     4741 gctgtgtatt ggtcattgat ttatccttcc gctcccgaaa aaatttatct ggttggaact
     4801 tcagttggat tgtattcaac gaatcagtta aacggaataa ataccatttg gcaacaggag
     4861 gcaccgggtg ttatcggtaa ttcaattgtg aatatgatca aagcgcgtgc ttcggatggg
     4921 aaaatggtag ttgctactca tggaaacgga atattttctg cacagttgac tcccctgttt
     4981 ttgtccagcc ccgaattaaa atcggcagaa cttcattttg caacctatcc caatccattt
     5041 gccgaggcaa ttacgattga tctttatttg ccctcagaag ggaaattgga tgttcgggta
     5101 ttcgacatca gcggtaggga aatcagggtg atttactcag gaacaagcgg tagcggtgag
     5161 cagcgcttaa tctggcaaag agaagatgca aacggcaggg aaatagcttc ggggacgtat
     5221 gtgttatctg tagcgcataa tggaaagcgt ctgacgcgga agctgattgt ccgttaattg
     5281 ctccttgtgt ccgattactc gtatatttgc aatgtttaat tgcaatgaga aagtcataca
     5341 taacccgcag ggaaaatttc aatgccgcgc acaagctgtg gcatgatgag tggtcggagg
     5401 agaagaactt cgaggttttt ggaaaatgtg ccaataaaaa ctggcacggt cataattata
     5461 acctgtttgt tacagtgaag ggagttcctg atcctgttac cgggtttata gttgatttaa
     5521 aagccttgag cgtattaatc cgcaatgaag taattgaaaa actggatcac cgaaatctca
     5581 atgtggatgt agattttatg aagggaatta agccgtccac cgaaaatctc gcgatcaaga
     5641 tctgggaaat ccttgaagag ccggtacgaa cactgggagg agaattgcac aatatccgac
     5701 tttgggaaac agaaaataat tacgcagaat atttcggaga ataagcattg atatggctaa
     5761 tccaactgac ataatttccg agaactcgct tgacggttac gaacgcgttg aacagtacaa
     5821 tgaaggcact ttgaaggaga tttcggatgc ttattcgaag atcattcctg cacttggtga
     5881 agatccaaag cgggaagggt tactcaaaac tccggaacgt gttgccaagg ctcttcagtt
     5941 tttaacacat ggttatgatc tgaatcccaa agagatactt caatccgcta tgtttgccga
     6001 agaataccgt cagatggtaa ttgtgaagga tattgaagtt tattccatgt gcgagcacca
     6061 tatgttacct ttttttggga aagcacacat tgcctatatc ccgaacggac atattgtcgg
     6121 actttccaag attccacgtg ttgttgatgc ctttgcacgg cgtttgcagg tacaggaaag
     6181 actgacaaca gagatccgcg actgtattca ggagacgttg agtcctcttg gagttgcagt
     6241 ggtgatcgaa gccagtcata tgtgcatgca gatgcgaggt gttcagaagc aaaattcagt
     6301 aaccacaact tctgctttca caggtgaatt cctgaaagac ccaacccgga aggaattcat
     6361 tacactgatt ggatcacatt taaaataata attcactaaa ttcgttataa cctaaaaacc
     6421 ataaccatga gcaacaatat gttcaatacc tcaaccaacg ttggaagcgg cggatcctct
     6481 tctttttccg gcgtaattcc ccgcacgttt gtagccaatg ttttctccta catgctggca
     6541 gcgcttgcca ttactggtgt agttgcatac gccatcggaa gtaatcttga gttgtttgtt
     6601 cgtctcttta tttccgaaac cggtcgtcca aacccattgt tctggatcgt atcatttgca
     6661 ccactcggat tggttctttt gatgaatttt gcgtttaacc gtttatcggc tccggtattg
     6721 ttaggcgttt ttatccttta tgcaggtatg atgggattgt cgcttagttc aatctttatc
     6781 atgtacacca ccggttcaat tgcagcagta ttttttatca ctgcaggtac atttggtgta
     6841 atggcattcc tgggttacac taccaaaaca gaccttacca aattcggatc cattctgatg
     6901 atgggactga tcgggataat tatcgcaagc atcgtaaaca tctttatgca aagctcaggt
     6961 cttgagtggt taatcagcat cattggagtg ttggttttca caggactcac agcatatgac
     7021 atgcagaaaa ttaaggaaat gggtgagcag gttggttccg gtcacgagct tgctagcaag
     7081 atggcgatta tggccggact tacactctac cttgatttca tcaacctttt cattatgctg
     7141 ctccgtttac tcggaaatcg caatgaataa gatcattgaa ttatagtttg aaaaaccccg
     7201 ctgtaagcgg ggttttttgt tttctacctt tttctatctt cgtccgtaaa ccactaatca
     7261 tgaaaaaggc atatgtgttt cccggacagg gatcccaatt ctccggaatg ggaaaggacc
     7321 tttatgagca atcaccgctt gcaaaggaaa tgtttgaaaa agcgaattcc attcttggat
     7381 ttcgaattac ggacatcatg ttttcaggat ctgatgaaga attaaaacaa accaaggtta
     7441 cccaacctgc tattttttta cactctgtaa ttcttgctgc taccttggga gaatctttta
     7501 aacccgacat ggttgcagga cattctctgg gggaattttc tgcactggtt gcaaacaaaa
     7561 cactttcgtt tgaggatgcg ctggtacttg tttcaaagcg tgcattagca atgcagaaag
     7621 catgtgaagc agaaccttcc actatggcgg ctgtgcttgg attggatgat gccaaagtag
     7681 aagcgatatg cactgcaaca gaaggtgtag ttgttgctgc gaattacaat tgcccgggtc
     7741 agctggtgat ttccggtgct gttgatgctg taaatcgtgc ttgtgaagca ttaaaagctg
     7801 caggtgcaaa acgtgcgtta ttactgcctg ttggaggagc atttcattct ccccttatgg
     7861 agcccgctcg cgttgaattg gaagcagcaa tcaatgcaac tcaattccat acaccggttt
     7921 gtccggttta ccaaaatgtg acagcaagtg cagttattga tccggaagca attaaaaaga
     7981 atcttgttgc gcagttgacc gcacctgttg catggacaca atcagtgcgc caaatgactg
     8041 cagatggtgc aacgcaatat gttgaagtag gtcccggaaa agtattgcag ggactcgtga
     8101 agaaaatttc gcccgaagta gaagtggcaa gtgcttaatg ctaatacagt aaaataaaaa
     8161 agtcccgttg ttgcgggact ttttttattt aatgagattg tattaaatct tgatctgaaa
     8221 aataa
//
LOCUS       DAOMYG010000087         8108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2946_length_8108_cov_9.119955, whole genome shotgun sequence.
ACCESSION   DAOMYG010000087 DAOMYG010000000
VERSION     DAOMYG010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8108
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2946_length_8108_cov_9.119955"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PK610_04155"
     CDS             <1..415
                     /locus_tag="PK610_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35152.1"
                     /translation="RSKRWSAIERKPFPLIYQGTPYTGILCTQLKSYALGKQNQKLTE
                     PLEEIQLNMDVDVDSIFLVYQNDSIQRMYRFAPDGNFITPKHYIIKPYSSALTPVFYI
                     YTSEEKKNKVYLIKHENLVWSKRKKAWTIRKKDLR"
     gene            429..1634
                     /locus_tag="PK610_04160"
     CDS             429..1634
                     /locus_tag="PK610_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRJ35153.1"
                     /translation="MKENKKIVVIGGGAAGFFGAINAAAFHPGAEILILEKGTQLLGK
                     VKVSGGGRCNVTHACFDPNELVRFYPRGEKELLGPFHQFMTGDTVAWFSERGVELKTE
                     SDNRMFPQSNSSQTIIDCFLSEAQKSGIQIRTQCGIHSLHRENEKWIVKTENETIAAD
                     KILLTAGSSIQVWELLRKLGHTIIDPVPSLFTFNCKDPRIATLPGISVPHAIVRIVGS
                     TIETEGPLLITHWGFSGPAVLRSSAWGANWLAEKNYSCTFMISWNGQTQKETLAELRT
                     LRDNEGKKICSASPLYEIPQRLWKTICDYCSITDIKWASLSNKQIELLAKEISAAEFQ
                     LEGKSTFKDEFVTAGGIDLAEVNMKTMESKLFPGLFFAGEILNIDAITGGFNFQAAWT
                     TSFIAAKSL"
     gene            complement(1641..2219)
                     /locus_tag="PK610_04165"
     CDS             complement(1641..2219)
                     /locus_tag="PK610_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRJ35154.1"
                     /translation="MKNKLRQHLEKSTALTDKEWGVLSSRLREQYLQRSSILSSAGRP
                     SEAMHFVVKGSVRSFINRDEGEITWNFYFPDGIATDFQSFLTGEPSPLDFETLEDTLL
                     ITLDKQSLPDMYKEAPELIRFENLFARRAFLDMRQRMESFLLQSAEERYLNFLNRHPD
                     IVSSLPGSYIATYLGLTPQSLSPIKRRLFKGK"
     gene            2349..5075
                     /locus_tag="PK610_04170"
     CDS             2349..5075
                     /locus_tag="PK610_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRJ35155.1"
                     /translation="MEPEKKKRWYSKVWKWARNTLLVLASIVAILVSLIVVFEEKIKR
                     YAISYLNQYLTTEVRVESIDLDVFSRFPNASLRFTNVVIMDPPHVKKLRDTLLYAKEL
                     HLEFGIWDLFGDDISVKNITALKADIRHYTDENGNANHEIWKEPETKSESTGFGFNLE
                     LLFLKDSKLRFVHKPGKQDHAFLLDELQLKGKFTEKAFAMKTGFKGTIRHIKNRGITL
                     IKNKSAEVQVGFDMDRNAKIHTISTGELLLGDMRFGIEGTIDDKEATQLDLAINGKDI
                     RIESLLKTFPELLGDDLEAYRSKGKLDFASSVKGPSADPDIAATFTISNAELSEKATG
                     IKLYDLNFKGDFKSKNKNEKERLLIEELSGKFSDGSFSGKLKLSDFERPFLFADLKGN
                     FNLATLHQFFKFKEVQKADGGLQIAARLETRFTHDTETEKWSMDVQKANGSAAVKDMN
                     LKLTDHHVAYKNLNGKFTLSNNNAFVEGLKGHLGKTDIAIDGVLENLVPYLLLRNEKL
                     TILADLRSELADLNDLFAIDNSEELKENNAATSGTDVKFPNNINFNLNATIGRIEYST
                     FNAGKIKGNLRMIDKVFQGKNLSMVFAKGHCSGELEVDGSNAKELGITGKVDISEMDI
                     RETFTLFRDFGQNVVKQENIKGLLSASVEFGFITDSSLRINEKKIISIAKIQITNGEL
                     IALQSLKDVSDYMRKDKKIKMALGKNIDDLEKRLSHIRFSELKNTLQIRNGKVIIPEM
                     EIESSALSIRFWGEHGFDNTIDYHINFRFRELKTKMEETEYGYIEDDGTGFRVYIHMY
                     GDINNPQFENDSKERKQDRKEYNQAEKQNIKAILKQEFGLYKKDSTLKINETPEQKVK
                     FMLEWEQDQPTDQPTDPNKDKTKRDNKDRMDKFKKKLGVQEDTSKTGFRHEQ"
     gene            5109..6677
                     /locus_tag="PK610_04175"
     CDS             5109..6677
                     /locus_tag="PK610_04175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRJ35156.1"
                     /translation="MNLYSRKQRWKIVLLFIALILTGASLWYSSKIVGKIREDERLRV
                     KIWSEAVRSSVNQLYVTGKLFDKLREDERKNVRRWAKAMQELGKDLNDYTFAMEIVQE
                     NKTIPVILTDSKGRYTSALNLDISEDSIRKNLKNARPDQDDVWYRNEARKIFEDTIYK
                     MVDEWKGNYPPIVINFRGKPINTVYYRESKLIAELEEKKDSLSKTFRQELVKNQALVP
                     VIFTDSTKTVVIETNFDSVEIATPGRLDELIAEMSKQNEPIMVRLNENEVGYIFYRDS
                     EVLRQLRLFPYIQLLIISLFILIGYLLFSTFRRAEQNQVWAGMAKETAHQLGTPLSSL
                     IAWLEILKGQGVDSQTISEMNKDIQRLQMVTDRFSKIGSEGKLESTDVQQLIVQSVSY
                     LRPRISSRVEIHIIEEVVPVMAKLNAPLFEWVIENLIKNATDAMEAEGKITIQFSSNL
                     NKVMIDISDTGKGIPHGNFKTIFEPGYTTKKRGWGLGLALAKRIVEYYHKGEIYVLRS
                     EPDKGTTFRIVLNK"
     gene            complement(6681..7235)
                     /locus_tag="PK610_04180"
     CDS             complement(6681..7235)
                     /locus_tag="PK610_04180"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRJ35157.1"
                     /translation="MNPLYTQRLIIRPVEREDIPALFAYRSDPEVSRYLQRDYHSVDD
                     VKYLVDETSRVADVPGTWFQLVVLAKDEGMIIGDIGIHFPAGDAQQSQVEIGYTFAKQ
                     HQGKGFAREAVSAVVEFIFAELNKRRITAGLDPENRASERLLQRIGFVKEAHFRKSYY
                     CKGEWVDDVIYALLKEEWKPGENR"
     gene            7441..>8108
                     /gene="hemW"
                     /locus_tag="PK610_04185"
     CDS             7441..>8108
                     /gene="hemW"
                     /locus_tag="PK610_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRJ35158.1"
                     /translation="MIYLHIPFCKQACHYCDFHFSTSMGFRSAVLEAMNREIEQRKEE
                     WNSGSMQTIYFGGGTPSLLSKEELTELLQTIRTNFIIDKNAEITLEANPDDITPEKLS
                     EWKAFGINRLSIGIQSFRDEDLKLLNRAHQAEEALQSIRNAQEAGITNITIDLIYGIP
                     GLEISDWIANLDLAIETDVPHISAYCLTVEPGTALESMVKKGKISPMNEDQGSEHFLI
                     LRDKL"
BASE COUNT         2622 a         1761 c         1704 g         2021 t
ORIGIN      
        1 acgcagtaaa cggtggagcg ccattgaacg gaaaccgttc ccgttgattt accaaggcac
       61 cccctatacc ggaatacttt gtacccaact aaaatcgtat gcactgggta aacaaaatca
      121 aaaattaaca gaaccccttg aggaaattca gctcaatatg gatgtcgatg tggatagtat
      181 atttctggtt taccaaaacg acagtatcca acgcatgtac aggttcgcac cggatggtaa
      241 tttcattacc cctaaacact acataataaa gccatacagc tctgccctaa cacctgtatt
      301 ttacatttac acttcggagg agaaaaaaaa caaggtatac ctgataaaac acgaaaacct
      361 ggtatggtcg aagcgaaaaa aagcatggac tattcgtaaa aaggatttga gataatgctt
      421 tgttgtttat gaaagagaac aaaaaaatcg ttgtaatagg tggaggagcc gcagggtttt
      481 tcggtgccat aaatgccgct gcctttcatc cgggcgctga gattctgatc cttgaaaaag
      541 gaacacaatt actgggtaaa gtaaaagttt caggtggcgg tagatgcaat gttacccacg
      601 catgcttcga tccgaatgag ttggttcgct tttaccccag aggagaaaaa gaattgcttg
      661 gcccctttca tcagttcatg acaggagata cggttgcatg gtttagtgag cgcggtgttg
      721 aattaaaaac cgaaagcgac aaccggatgt ttcctcaaag caattcatca caaaccataa
      781 tcgattgttt tctttcagaa gcacaaaaat caggaataca aattcgtact caatgtggta
      841 ttcattccct tcacagggaa aacgagaaat ggattgtaaa aacagaaaac gaaaccattg
      901 ctgccgacaa aatacttctc acagcaggta gtagtataca ggtatgggaa cttctgcgca
      961 aattgggtca caccattatc gaccctgttc cttcactttt cactttcaac tgtaaagatc
     1021 cgcgaatagc tacattgcct ggaatttccg ttccccatgc aattgtgcgg attgttggaa
     1081 gtacaatcga gacagaaggc cccttgctaa ttacacattg gggatttagc ggtcctgccg
     1141 ttctgcgttc ctctgcctgg ggagcgaact ggctggctga aaaaaactat tcctgcacat
     1201 ttatgatcag ctggaatggt caaactcaaa aagaaacatt agcagagctt cgcaccttgc
     1261 gcgacaacga gggtaaaaaa atatgcagcg catcacccct gtacgaaatc ccgcaacgac
     1321 tttggaaaac aatttgtgat tattgttcca taaccgacat caaatgggct tcgctatcca
     1381 ataaacaaat tgaacttttg gcaaaagaaa ttagtgctgc agaatttcag ctcgaaggaa
     1441 aaagcacgtt taaagacgaa tttgtaaccg ccggcggaat tgatcttgcg gaagtaaaca
     1501 tgaagaccat ggaaagcaaa ctgtttcccg gtttgttctt tgccggagaa atattgaaca
     1561 tcgatgccat caccggtggg ttcaactttc aggcggcatg gacaacctcg ttcatcgctg
     1621 ctaagagcct ttagaaaaat ttatttcccc ttaaaaagtc ttcgtttaat agggctgaga
     1681 ctttgcggag ttaatccaag gtaagttgcg atataactac ccggaagact gcttacaata
     1741 tcgggatgac ggttcagaaa attcaaatag cgctcttcgg cagattgaag aagaaaggac
     1801 tccatgcgct gacgcatatc cagaaatgcc cgccgtgcaa aaagattctc aaaacgaatc
     1861 aactcaggcg cttctttgta catgtcaggc aaagattgct tatcaagcgt gatcaataaa
     1921 gtgtcttcaa gggtttcgaa atccaaagga ctgggctcac ctgtgaggaa actctggaag
     1981 tcggttgcta ttccatcggg aaaataaaag ttccaggtaa tctcaccttc gtccctgtta
     2041 ataaaggatc gaaccgagcc tttcacaaca aaatgcatgg cttcactcgg ccttcccgct
     2101 gaggataata tagaagaacg ttgcaagtac tgttcccgta atcgggagga taagacaccc
     2161 cattccttat cggtcaacgc tgtgctcttc tccaggtgtt ggcgaagttt gtttttcatc
     2221 gttaccaaaa tacgattttc tccctcatta cgttagtatt caggtaatgc tgtattaaca
     2281 tccgtctttg cataaatgca tttagggcga atcgtattgc atgtccttgc agactaactt
     2341 gcagtcgaat ggaaccggag aagaagaagc gttggtacag caaggtctgg aaatgggccc
     2401 ggaatacgct attggttctt gcaagcattg ttgccattct cgtttcactt atcgttgttt
     2461 ttgaagaaaa aattaaacga tatgcgatta gctatctcaa tcaatacctt accacagaag
     2521 taagagtaga gtccatcgac ctcgatgttt ttagtcgttt tcccaacgct tccttgcgct
     2581 ttaccaatgt agtcatcatg gatccgccac atgtaaaaaa gctgcgcgac actcttttgt
     2641 acgctaaaga gctgcacctc gaatttggga tttgggattt atttggggat gatatcagcg
     2701 taaaaaacat taccgcactt aaagccgaca ttcgccatta caccgatgaa aacggaaatg
     2761 ccaatcacga aatatggaag gagcctgaaa caaaatcgga atcgacaggg ttcggtttta
     2821 atctcgagct tttgtttctt aaagacagca aacttcgctt cgttcacaag cccggtaagc
     2881 aggaccatgc atttctgctt gatgaactgc aactaaaagg caagttcacg gaaaaagcct
     2941 tcgcaatgaa aacaggcttt aaaggaacca tccgccatat taaaaacaga ggcatcacac
     3001 ttataaaaaa caaaagcgcc gaagtacagg taggtttcga tatggaccga aacgccaaaa
     3061 tacacaccat tagcacagga gaacttttgc tgggcgacat gcgctttggc attgaaggta
     3121 caattgatga taaggaagca acacaactgg atctggccat caatggtaaa gacatacgca
     3181 tcgaatcgct tttgaaaaca ttccctgaat tattagggga cgatctggaa gcctaccgct
     3241 ccaaaggcaa attggatttt gcgtcgtccg taaaaggacc ttcagctgat ccggatattg
     3301 cggcaacatt caccatcagc aatgcggaac taagtgaaaa agcaaccggc atcaaattgt
     3361 acgatctcaa tttcaaaggt gatttcaaaa gcaaaaataa aaatgagaaa gagcgcttac
     3421 tgatcgaaga attatccggg aaattcagcg atggttcgtt tagcggcaaa ctaaaactta
     3481 gtgattttga gcgccctttt ttattcgccg acctaaaagg aaattttaac ctcgcaacac
     3541 tccatcaatt tttcaaattt aaggaagtgc aaaaggcaga cggtggattg caaatagcag
     3601 cccgattgga aacccggttc acacatgaca ctgaaacaga aaagtggagc atggatgtac
     3661 aaaaggcaaa tggaagtgca gcagtaaagg acatgaacct taaacttacc gatcatcatg
     3721 ttgcttataa aaacctaaat ggaaaattta cactcagcaa caataatgcg tttgtagagg
     3781 gactgaaagg tcatctcgga aaaacagaca tcgctattga tggcgtactt gaaaatttag
     3841 tcccgtacct gttacttcga aatgaaaaac taaccatact ggcagatctt cgttctgaac
     3901 tggcagatct taacgatctt tttgctattg acaatagcga agagcttaag gaaaacaatg
     3961 cggcaacatc cggtactgat gttaagttcc cgaataatat caattttaat ctcaatgcta
     4021 ctataggaag gatcgaatac agcacattta atgcaggtaa gatcaaaggg aacttacgga
     4081 tgatagataa agttttccaa gggaaaaacc tgagcatggt gtttgcaaaa ggccactgtt
     4141 caggagaact tgaagttgac ggaagcaatg caaaagaact tggaattaca ggaaaagtag
     4201 atatttctga aatggacatc cgtgaaacat tcacgctgtt ccgggatttt ggtcagaacg
     4261 ttgtaaaaca ggaaaacatt aaaggactgt tatccgcatc agtagaattt ggatttatca
     4321 cagactcctc cctgcgcatc aacgaaaaaa agatcatcag tattgcaaag attcaaatta
     4381 ccaacggaga attgatcgca ttgcagtcac tgaaagatgt ttccgattac atgcggaaag
     4441 acaaaaaaat caaaatggcc cttggcaaaa atatcgatga cctggaaaaa cgattgtcac
     4501 acatccggtt ctctgaatta aaaaatacgc tccagatcag aaacggaaaa gtaattatcc
     4561 cggagatgga aatagaatcg tcagccctga gtattcgctt ttggggcgag catggttttg
     4621 ataatacgat tgactaccat ataaatttca gattcagaga gctaaaaacc aaaatggaag
     4681 aaaccgaata cggatacatc gaagacgatg gtacgggttt cagggtatat attcatatgt
     4741 atggcgacat taacaatccc caattcgaaa acgacagcaa agaacgaaaa caggatcgca
     4801 aggaatacaa ccaggcggaa aaacaaaaca tcaaagccat cttaaaacag gaatttggct
     4861 tgtataaaaa agacagcacg ctaaaaataa atgaaacgcc tgaacaaaaa gttaagttca
     4921 tgctggagtg ggaacaggat caaccaaccg accagccaac cgatccgaat aaagataaaa
     4981 ccaaacggga caataaagac aggatggaca agtttaaaaa gaagcttggg gtacaggaag
     5041 acacttcaaa aaccggtttc cggcacgaac aataatactt atcttggtaa tcataaacta
     5101 ctgatccgtt gaatctctat tcgcgtaagc aacgctggaa gattgtatta ttatttattg
     5161 cgcttattct tacaggtgct tcgctttggt attcaagtaa aatagttgga aagatccggg
     5221 aagacgagcg tttgcgtgtc aaaatatggt cggaggctgt tcgttcttcg gtgaatcaat
     5281 tgtatgtaac aggtaaacta ttcgataagc ttcgtgaaga cgaaagaaaa aacgtgcgtc
     5341 gctgggcaaa ggcaatgcag gaacttggca aagacctgaa cgattacacc tttgcgatgg
     5401 aaatcgtaca ggaaaataaa accattcctg ttattctaac cgatagtaaa ggccgataca
     5461 cctcagcact taatctggac atcagtgaag atagtatccg aaaaaatctg aaaaacgccc
     5521 gtcctgatca ggatgatgtg tggtaccgca acgaggcgcg taaaattttt gaagacacca
     5581 tctacaaaat ggtagatgaa tggaaaggaa actatccccc tattgttatc aattttcgtg
     5641 gtaaacccat caataccgtt tactaccgcg aatcaaaatt aattgcggag ctggaagaga
     5701 aaaaagattc gctcagcaaa acatttcgtc aggaactcgt aaaaaaccag gcgctggtcc
     5761 ccgttatatt tacagattca actaaaacag tagttattga aaccaatttc gattctgtag
     5821 aaatagcaac ccccggaaga ttagatgaat taattgctga aatgagtaag caaaacgagc
     5881 cgataatggt tcgtctgaat gaaaatgaag tcggatacat tttctaccgc gactctgaag
     5941 ttttgcggca attgcgcttg tttccctata tccaattgct cattatctca ctgtttattc
     6001 ttataggcta tcttttgttt tccacatttc gaagggcaga acagaatcag gtttgggcag
     6061 gaatggctaa agaaacggct catcaattgg ggactccttt atcctcgctt attgcctggc
     6121 tggaaatttt aaaaggtcag ggagtagatt ctcaaaccat aagcgagatg aataaggata
     6181 ttcagcgctt acaaatggta acagaccgct tctccaagat tggttcagaa ggcaagctgg
     6241 aatcaacgga tgtacagcag ctcattgtgc aatccgtttc gtatttacgc ccccgtattt
     6301 cgtcgcgtgt tgaaattcat atcatcgaag aggttgttcc cgtaatggct aaactcaacg
     6361 cccccttgtt tgaatgggta atagaaaacc tgataaaaaa tgctactgat gccatggagg
     6421 cagaggggaa aataaccatt caattcagca gcaacctcaa caaggtaatg atcgacatca
     6481 gcgataccgg aaaaggaatt ccacatggaa actttaaaac aatttttgag cccggttaca
     6541 ccaccaaaaa acgcgggtgg ggattgggtc tggcgcttgc caaacgcatt gtagaatatt
     6601 accacaaagg agaaatctac gttctgcgct ccgaacccga taagggtacc actttcagaa
     6661 ttgtgctgaa taaataatcc ttaacggttt tctccgggtt tccattcttc tttcagcaag
     6721 gcgtagataa catcatctac ccactcgcct ttgcaataat aactcttcct gaaatgggcc
     6781 tcctttacaa atccgattcg ctgtaacaaa cgctccgaag ccctgttttc agggtcgagt
     6841 cctgctgtaa ttctccgctt attgagttcg gcaaaaataa attcaaccac cgccgaaacc
     6901 gcttcccttg caaatccttt tccctgatgt tgctttgcaa atgtatatcc aatctccacc
     6961 tgactctgct gtgcatcacc cgccggaaaa tgaatgccga tgtcgcctat gatcattcct
     7021 tcgtccttag ccaacactac caattggaac caggtgcccg gaacatccgc tacacgtgat
     7081 gtttcatcta ccaaatactt cacatcatct acggaatgat aatcgcgctg caaataccgc
     7141 gatacttccg gatctgaacg atacgcaaat aatgcaggta tatcctcccg ttcaacagga
     7201 cgaatgatca gacgttgggt gtaaagcgga ttcataactg atatcaacca caatactaca
     7261 acatttaggt cctacaaacc aactatcttc ataatcataa aaacctcctc cacaagttct
     7321 atcttcgcaa actaaaccta tcagcacatg gttcgacctc aagggagaac catcctgacc
     7381 gacctgcccc gaccgtagcg tcggggtaac gtcaggattc ccacattcgc acactaaaaa
     7441 atgatctacc tccacatccc tttctgcaaa caagcctgcc actactgcga ttttcatttc
     7501 agtacatcga tgggtttccg ttctgcggta ctggaggcca tgaacaggga aatagaacaa
     7561 cgcaaggaag aatggaacag cggctcaatg caaaccattt acttcggcgg aggaactccc
     7621 tcgctcttga gtaaagaaga gctgaccgaa ctcctgcaaa cgattcgaac aaattttatc
     7681 atcgataaaa atgcagaaat cacgctcgag gccaatcccg acgatatcac gcctgaaaaa
     7741 ctaagtgaat ggaaagcctt cggcatcaac cggctcagta ttggcataca atcctttcgt
     7801 gatgaagatc tcaaattgct aaatcgcgca catcaggctg aggaagcatt acaatccatc
     7861 cgcaatgcgc aggaagccgg gatcaccaac atcactatcg atctcattta cggaataccg
     7921 ggactcgaaa tctccgattg gattgccaac ttagacctgg ccatcgaaac ggatgtgcct
     7981 cacatatcgg cgtattgcct gactgttgaa ccgggtactg ccctcgaaag catggtgaaa
     8041 aaaggcaaga tcagtccgat gaatgaagat cagggttctg agcacttttt gatactgcgc
     8101 gataaact
//
LOCUS       DAOMYG010000088         8097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2952_length_8097_cov_9.022756, whole genome shotgun sequence.
ACCESSION   DAOMYG010000088 DAOMYG010000000
VERSION     DAOMYG010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2952_length_8097_cov_9.022756"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1750
                     /locus_tag="PK610_04190"
     CDS             167..1750
                     /locus_tag="PK610_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35159.1"
                     /translation="MIKQLFITLFFACVSVFGDAQTFSWAQCTVNTGMEYIDVCVGKN
                     NSILVLADYTSLSYRTAEEIFFDSKGSVKIDQLWRSSQLLFCFDSEGKLKWYTSIVGK
                     SNRVEKITSTKSGGFVLLADIEGSHSAIPGAKQVKNQASPSRSNDDDDDEEEEEEDET
                     DSDDFPLRYGKFQLNNQSGLFCRAGWNLVHLSADGRLIQIDSLGWNEEQDFEMSQFKS
                     HPDGDFLISGHFVKGKLSPSPDHAAGVSGAMFLAKVGADGKLRWADVSRSQSPSCCSY
                     RGDLSHVSISADGWIVHSGTLYGGGVFGGQIVTSDLRYSAGAGSSDFMDGFLACYDPK
                     GKLQWVKTAGNISGFCNAVVSTSDRVFISYNLHNGEQLYGMKMDTSLRRKSFLLSFDK
                     KGNFLSVRSMTTTIHHMIPSGENGLIIYGDADRYNKHRVFDDRVSFGERDLMFLATMD
                     KNLNVTGFHGFRLLVSRNGEQDLIPLSNGDVFMWGQLWGALPIEANILDKAFPAAKVY
                     GGSPYCGYISKDVFTPIKK"
     gene            2184..5444
                     /locus_tag="PK610_04195"
     CDS             2184..5444
                     /locus_tag="PK610_04195"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRJ35160.1"
                     /translation="MRYLILVSFCLLFTDGSAQKADSLLAEWKSYKLLPKSNFSQDTN
                     AVKVCLALTRESSVNGDSALLFVAEARRISQNVKWIKGLAMTDYRKAVMLSSMGDQDA
                     AIVLFTASAENWKKVLPPSGGIDAAQCLSRIGSCYVNSGKPKEALPFFEKSIELYTRL
                     DIYTGIVSQCNLLGLANQSMERDSIAIYWFSKGIPICEKHNDKLRKGILLGNIGLSHA
                     SLGEYSKALQVYDQALKIDEELGNFNGQLRHIGNMAASYRAMGELELALNLAQKALKI
                     SRELNNQRGISSNLNGIAIIQCLIGDYQKALENNFEALEIDTKLGHLSGIQSEVGNIG
                     LTYQYIGDAPLALVYHAKSLEMAEQAGSLSGQISTLNNIGIVYFMQNLYSKALEIHLR
                     ALELCNQHQNKKDFVYVLGSIANDYKGLKKLDTAVLFYERAIVYCKELGMNANLAHNT
                     ANMAEVYLDKKEFKKSLQNNTEALVMYQKMGMLPNEVRSYGNIAMTYHKMNDHVLAEK
                     NAVVSLEKAINISTPEVIRYASRIGYIVSLKSNTPQASLNRLSLLRKSSWQQMELSYF
                     SLSEREREEFFELVESDFGDYFDFTVKHHRQFPALTDTMYNIVLGIKGLSLRSNTYLR
                     NSILQSKDTVLISQYNRLVELKNRIARELASGVRNIPLENEANELERSVIASSKVFSD
                     FDRIKKLDWKDVRNGLKKDECAIEFVDYETRFDSSSSVLYAALIVKKDSEHPELVQLC
                     TQKELERILGNTQEKNVNYVKRLYGTKGKYRSELYAKIWQPLEAQLKGIKKIYYSPSG
                     LLHTVSFSALSGGGNSFIADNYNLQHYSSTGIIAFAANQMQLKNAEVLLMGGIDYSGS
                     NGKGWSYLPGTEEEVKTLNSLFTANKLNTSYYSSLNASEAIVKERGAKADIIHIATHG
                     FSFSQGKPENKSEYVEYGDVVFRGTGSYADLSFISNSNPMMRSGLVMAGANTVWERDP
                     ASKTEDGILTALEVSNLNLSKTKLVVLSACETGLGQVKGSEGVYGLQRAFKMAGAQFV
                     IMSLWQVPDKETSEFMKLFYGRLLKTNDVRMSFENAQKSMRKKYDPYYWAAFVLVE"
     gene            complement(5445..5951)
                     /locus_tag="PK610_04200"
     CDS             complement(5445..5951)
                     /locus_tag="PK610_04200"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRJ35161.1"
                     /translation="MKSKKQTEYPFLAEVLRETSGIRFHYIEFPLSAEKLFGKKGSIR
                     VILTLNKASFKRSLMPQSSGFHRLILGGDIRKKTGISEGDTVSCSLRFDPEIPELIIP
                     EELDAIFEMEPEINQQFNKLPPGMKRQICTWVAEGKKIETRVNRSLEIMKRFQNGKFH
                     FGGKESTR"
     gene            6411..7292
                     /locus_tag="PK610_04205"
     CDS             6411..7292
                     /locus_tag="PK610_04205"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRJ35162.1"
                     /translation="MIYAVMLAYWGVGLTAGAAMLGLNIFSALYYHVYSLEMNVSSLR
                     TIDAGVKTALIVEIVVVMLAIALLLYQFVNNYRISLSKLSDVNEELKEANAMLSAKNA
                     ENVLLLKEVHHRVKNNLQIIISLLRLQSGGLPKESMERFDDAINRILTMALIHRKLYQ
                     SSDLSVIEIEAYVRDLVSGLTDKETIKLTVDSEVKNFGLKTIVPLGLMLNELLSNSYK
                     HAFKEKESGEIILRIRTHGTDAFLLEYSDSGIWIESEASSERFGLNLIETLTEQMEGS
                     IERKGSSYVFKLKNLDI"
     gene            7384..>8097
                     /locus_tag="PK610_04210"
     CDS             7384..>8097
                     /locus_tag="PK610_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35163.1"
                     /translation="MIVGARLSAFGGSIPIISSSGAVVDGHELPKGKLVELMHPAQYA
                     GQYCFIQTSKGWGLYDRNFSNWVLDPEFDFLEQLDEWIVVVKNAKVRMFHLRTRTYLN
                     DYDAGFSAILTAYDGDKSWYVQEGHKWKNLTWIEGKPSIPKDQEGYFDKKAYTTHSVK
                     IEEGKILVEVRTRFPKPAFPLVNIDAFGTAYDSLDKHGNLVYPPHSPDLIGVSIYDLK
                     SKKSIIRSTDYYKANLLNGR"
BASE COUNT         2386 a         1400 c         1833 g         2478 t
ORIGIN      
        1 taatggctga ccgaaaattt ggtttggctg ttgaggatgg aaaaatttta gttcaacctt
       61 cctaccagac aatacatcga cctgtattga atgagtcttt gttagagatc aaacaaggtg
      121 aggttttgtg ctacggtgat ttagaaggta aagtgtacaa agacgaatga taaaacaact
      181 tttcataaca ctgttttttg cgtgtgtatc tgtttttgga gatgcgcaga ccttctcatg
      241 ggcacaatgc acagtgaata ccggcatgga gtatatcgat gtttgtgtgg gcaaaaacaa
      301 ttcaatactg gtgttggctg attatacttc actcagttat cgaacagctg aagaaatatt
      361 ttttgattct aaaggaagcg ttaaaataga tcagctatgg cggtcatcac aattgttgtt
      421 ctgttttgat tcggaaggca aactgaaatg gtacaccagc attgtgggaa agagcaacag
      481 agtagaaaag attacctcta caaaaagcgg tggatttgta ttgctggccg atattgaagg
      541 atcgcattca gcaatacccg gagcaaaaca ggtaaagaat caagcatctc catcacgtag
      601 caatgatgat gatgacgatg aagaagagga ggaggaggat gaaacggatt cggatgattt
      661 tcctttgcgt tacggtaaat ttcagctcaa taaccaaagt ggtttgtttt gcagggcagg
      721 atggaacctc gtacacctga gtgctgatgg aagactaatc caaattgatt cattgggctg
      781 gaatgaggaa caggattttg agatgtcgca gtttaagtca caccccgatg gggattttct
      841 catatcagga cattttgtca aggggaaatt atcgccttct cctgatcatg cagctggagt
      901 ttcgggtgcg atgtttcttg caaaggttgg tgcagatgga aaattacgct gggcagatgt
      961 aagcagaagt cagtcgccat cctgctgctc ctatcgggga gatctttcac atgtttcaat
     1021 ttctgccgat ggctggatcg ttcattccgg aacattgtat ggaggtggtg ttttcggcgg
     1081 acaaatcgtc acttccgatc tcaggtattc tgcaggagca ggctcttcgg attttatgga
     1141 tggttttttg gcttgttatg atccgaaagg aaaattacaa tgggtaaaaa cagcgggaaa
     1201 tatcagcgga ttttgcaatg cagttgtttc gacctctgat cgggttttta tcagctataa
     1261 tcttcataat ggggaacaac tgtacggaat gaaaatggac accagcttgc gtagaaaaag
     1321 ttttcttttg agtttcgaca aaaaaggcaa ttttcttagt gttcggtcaa tgactaccac
     1381 gatacatcat atgattccat ccggtgaaaa cggattgata atttacgggg atgcagaccg
     1441 ctacaataaa catcgtgtat ttgatgatcg tgtttcgttt ggcgaaaggg atttaatgtt
     1501 cctggccacg atggataaga atttgaatgt tacaggcttt catggcttta gattactggt
     1561 gtcgcgaaat ggtgaacagg atttgattcc gctttcaaat ggagatgtat tcatgtgggg
     1621 acagctttgg ggggctttac ccatagaagc gaacatatta gacaaagcct ttccggcagc
     1681 taaagtctat ggtggatcac cctattgcgg gtatatcagt aaagatgttt ttactccgat
     1741 aaaaaaataa agggtgacaa ttgccacccc taacactgaa attttttgtt taccacaccg
     1801 aagattttcc aatgttctgc tggaggattt cataaccttg tccgaggcgt tttggatgtg
     1861 cccggaaatt gttgacatcc ccggaatcag gctaaatgca gaaacacaat ttcaaagcaa
     1921 caagggctca tcaacttgtt ttttttgaga tgttgtaggg atgcgagtct gtacattgcc
     1981 taactatgat tttgtattga cccacctgat tatttatttt taaccaatta tggttgagcg
     2041 tataccttta aattcaagcc gtacttgtga attttattga aataggaatt caacattaac
     2101 gtattgtgcg gatcacagca agcttgttca tagttgcaat ttattattct ttatatttcg
     2161 actctctatt ttatattcta tccatgcgtt atttaattct agtctctttc tgtttactat
     2221 ttacagacgg ttctgcacaa aaggcagatt cacttttagc tgaatggaaa tcatacaaat
     2281 tgttacccaa aagcaatttt agccaggaca ccaatgctgt aaaagtttgc ctggcattaa
     2341 ctcgtgaatc ctcagttaac ggcgattctg cactgttatt tgttgcagaa gcccggcgaa
     2401 tttcacagaa cgtgaagtgg ataaaaggac tggcgatgac ggattaccga aaggcagtaa
     2461 tgctttcatc gatgggagat caggatgctg ccattgtttt atttaccgcc tctgctgaaa
     2521 actggaagaa agttcttcct ccatcaggtg gaatagatgc agcacaatgt ctttcccgga
     2581 ttggaagttg ctatgttaac tcgggaaagc caaaagaagc cttgccattt tttgaaaagt
     2641 caattgaatt atacaccagg ctggatattt ataccggaat agtttcacaa tgtaatctat
     2701 taggtttggc caaccagagt atggaaaggg attctattgc catttattgg ttttcaaaag
     2761 ggattccaat ttgtgagaag cataacgata agctgcgaaa gggaattttg ttagggaaca
     2821 ttgggctctc acatgcaagt ttaggagaat attcaaaagc acttcaggtt tacgatcagg
     2881 cactcaagat cgatgaagaa ttggggaatt tcaatgggca gttgcgacat atcggtaata
     2941 tggcagcatc gtatcgtgct atgggtgaat tggaattagc attgaatctt gcacaaaaag
     3001 ctttgaaaat tagcagagag ttaaacaacc aaagagggat ttcaagtaat cttaacggaa
     3061 ttgcaattat tcaatgtctg attggagatt accagaaagc cttggagaat aattttgaag
     3121 cgcttgaaat tgatacaaag ctggggcatc tttcgggaat tcaatcggag gttggcaata
     3181 tcggattaac ctaccagtat attggcgatg caccattggc tttggtttac cacgcaaaaa
     3241 gtcttgagat ggcagagcag gcagggtctc tctccggtca gatcagcaca ttgaacaata
     3301 tcggaatcgt ttattttatg cagaatttgt attccaaagc attggaaatt catttacgtg
     3361 cactagagtt atgcaatcag catcagaaca aaaaggattt tgtttatgta ttgggtagca
     3421 ttgcgaatga ttataaggga ttaaaaaaat tggatacagc ggtgttgttt tatgaacgcg
     3481 ccattgtcta ttgcaaagaa ttgggaatga atgcaaatct tgcgcacaat accgctaaca
     3541 tggcggaggt ttatcttgat aagaaggaat tcaaaaaatc cttgcagaat aatacagagg
     3601 cattggtaat gtatcagaag atgggaatgc ttcctaacga agtacggtcg tacggaaata
     3661 ttgcaatgac ctaccacaaa atgaatgatc atgtacttgc agaaaagaac gctgtagttt
     3721 ccttggagaa ggccattaac atttcaactc ctgaggtgat tcgttatgca tcacgtattg
     3781 gatatattgt tagtttgaag agcaacacgc cacaggcttc attgaacagg ctttctttat
     3841 tgagaaaatc ttcctggcaa caaatggagt taagttattt ttcattgtcg gaacgcgaac
     3901 gcgaggaatt ttttgagttg gtggaaagtg attttggaga ttattttgat tttacggtta
     3961 agcatcatcg acaatttcct gcactgaccg ataccatgta caacattgtt ctggggataa
     4021 aaggactctc gcttcgatcg aatacctatt tgagaaattc tattttacag agcaaggaca
     4081 ctgtattgat cagccagtat aatcggcttg tggaattgaa aaacaggatt gcaagggaac
     4141 ttgcttccgg ggttcgtaat attcctttgg agaatgaagc caacgaattg gaacgatccg
     4201 ttattgcctc atcgaaagta ttcagtgatt ttgaccgaat taaaaagctc gactggaagg
     4261 atgtacgaaa tggattaaag aaagatgaat gtgcaattga gtttgtagat tacgaaacgc
     4321 gttttgattc atcttcatcg gttctttatg ctgcattaat cgttaagaag gatagtgaac
     4381 accccgagct tgtgcaattg tgcacgcaaa aggagcttga aaggattctt ggaaatacac
     4441 aggagaaaaa tgtgaattat gtgaagcgct tgtatgggac gaaaggaaag tacagatctg
     4501 aattgtatgc gaaaatctgg cagccacttg aggcgcagtt gaaagggatt aaaaaaatct
     4561 attattcccc gagtggttta cttcatactg tttccttttc tgcattaagc ggaggcggta
     4621 attcctttat cgctgataat tataatttac aacattactc aagcaccggc ataattgcgt
     4681 ttgcagcgaa tcagatgcaa ttaaagaacg cagaggtatt gctgatgggt ggaatcgatt
     4741 attcgggctc aaatggaaaa ggctggtcgt atttaccggg tacagaagaa gaagtgaaaa
     4801 cactgaattc actttttacc gcaaataagc tgaatacgtc ttactactct tcattaaacg
     4861 caagcgaagc aattgttaag gaaaggggtg caaaagcaga tatcattcat atcgcaacac
     4921 atggattttc gttttcgcag ggtaaaccgg aaaacaaatc tgaatatgtg gaatatgggg
     4981 atgtggtttt taggggaaca ggttcgtatg cggatttaag tttcatcagt aattccaatc
     5041 ccatgatgcg atctggtttg gtgatggctg gtgcaaatac agtttgggag cgagatcctg
     5101 cttcaaaaac agaggatgga atcttaaccg ctttggaagt ttccaatctt aatttgagta
     5161 aaacaaaatt ggttgttctt tctgcctgcg aaacaggctt gggtcaggtg aagggaagtg
     5221 aaggcgtgta tggattgcaa cgcgcattta aaatggcagg agcgcaattt gttattatga
     5281 gtctttggca ggttcccgat aaggaaacct cagagttcat gaagttgttt tatggccgcc
     5341 ttctaaaaac gaatgatgtt cggatgtcat ttgaaaatgc gcaaaaatct atgcgcaaaa
     5401 agtatgatcc gtactattgg gcagcattcg tattggtgga gtaatcagcg agtactctcc
     5461 ttgccgccga aatgaaattt tccattttga aagcgcttca tgatttcaag tgaacgattg
     5521 actctagttt caattttttt gccttctgct acccatgtgc aaatctggcg tttcattccc
     5581 ggtggcaact tattgaattg ttgatttatc tccggttcca tttcaaaaat ggcatcaagt
     5641 tcttccggaa ttatgagttc cggaatttca ggatcaaatc gtaaactgca tgaaacggtg
     5701 tccccttcac ttattccggt ttttttgcgg atatctcccc caagtatcaa tcggtgaaaa
     5761 cccgaagatt gtggcatgag tgatcgttta aacgatgctt tatttagagt gagaattaca
     5821 cgtatcgaac ccttttttcc gaataacttt tctgctgata gtggaaattc tatataatga
     5881 aatcgaattc ctgatgtttc acgtaacact tctgctaaaa atggatattc tgtttgtttt
     5941 ttcgatttca tgatctaatc taataaaaat taattgatac aatcctgttg ggactcagga
     6001 tactggcaat tgaaaagtct tctatgcctg ccaggccctg aaaacatgtt gatatattgt
     6061 tatttttaag atgtgaaacg gaatggctac acctggccac gagatttatg cctactacat
     6121 ctgctgaaca aaaacatttt gaaataagta agatccggct tgctgttcgg ctttgtgctt
     6181 tattgtgttg catgtttgga ataatgacag caatcgaatt ttttggatca cttcaaaatt
     6241 tttatctcga tggattttgt tttttgcttt cgcttggatt tctactattt cttctgaaaa
     6301 caaaaaatta taaacctatt tatctaatag gctctttctc aataaccatt atttgttttt
     6361 tttcacttaa taattttctc cacgatacgc atcatgccgt tttcctttgg atgatttatg
     6421 cagtaatgct tgcttattgg ggtgtaggcc ttactgcagg tgcagctatg cttgggctga
     6481 atattttttc ggccctttac taccatgtgt attccctgga gatgaatgta agttcattac
     6541 gaactataga tgccggtgta aaaacagcac ttattgttga gatagtggtt gtaatgctgg
     6601 caattgcatt gttgttgtat cagtttgtga acaattacag gatctcccta tcaaaactca
     6661 gtgatgttaa tgaggagttg aaggaagcaa acgccatgct ttctgctaaa aatgcggaaa
     6721 atgttctttt gttgaaggag gtacatcaca gggtaaaaaa caatttgcag atcatcatta
     6781 gtttgcttcg cttgcaatca ggtggacttc caaaagaatc aatggagcgg tttgatgatg
     6841 ctattaacag aatccttaca atggccctga tacatcggaa actttatcag tcgagtgatt
     6901 tgtcggttat tgaaattgaa gcctatgttc gcgatttggt ttccggatta acagataaag
     6961 aaacaattaa attgacagtt gattcagagg ttaaaaactt cggcctaaag acaattgttc
     7021 ccctgggatt aatgctgaac gagttgttat ccaactccta taaacatgct ttcaaggaaa
     7081 aggaaagcgg agaaattatt ctacgaataa ggactcatgg aacggatgct tttttgcttg
     7141 aatacagcga tagcgggatc tggattgaaa gtgaagcatc atccgaacgt ttcggtttga
     7201 atttgattga aaccctgacc gaacaaatgg aagggagtat tgaacgaaag ggctcttcat
     7261 atgtgttcaa actcaaaaat cttgatattt aattagggta aacaccttag taaccaattc
     7321 ccataatccg gttatattag tgttatcaag atgtttacta tgcttcgtta tctgcttata
     7381 ttaatgattg tcggtgctcg cttgagtgct tttggaggat ctattccaat tatcagttct
     7441 tccggcgctg ttgtggatgg gcatgaatta cctaagggta agttggtgga attgatgcat
     7501 ccggcgcagt acgcaggaca atattgcttc atacagacct ctaaaggctg gggattatac
     7561 gatcgcaatt tttcgaactg ggtattggat cctgaatttg attttctgga acagctcgat
     7621 gaatggattg tggtggtaaa aaatgcgaaa gtccgtatgt tccatttgcg gacccgtacc
     7681 tatcttaacg attacgatgc cggtttcagt gcaattctta cggcatatga tggtgataag
     7741 tcctggtacg tgcaggaagg tcataagtgg aaaaatctga cttggataga aggtaagccg
     7801 tcaataccaa aagatcagga aggctatttc gataagaagg cctatactac acacagtgta
     7861 aaaattgaag agggaaaaat tcttgttgaa gtccgtacgc gatttcccaa acctgccttt
     7921 cctctggtga acatcgatgc attcggaaca gcgtacgatt cgctggacaa acacggcaat
     7981 ctcgtttacc ccccgcatag tccggattta attggtgttt cgatttacga tttaaaatca
     8041 aaaaaatcga tcatacgttc aaccgattat tacaaagcca atttgctgaa cggaaga
//
LOCUS       DAOMYG010000089         8055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2969_length_8055_cov_8.855875, whole genome shotgun sequence.
ACCESSION   DAOMYG010000089 DAOMYG010000000
VERSION     DAOMYG010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8055
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2969_length_8055_cov_8.855875"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..705)
                     /locus_tag="PK610_04215"
     CDS             complement(118..705)
                     /locus_tag="PK610_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRJ35164.1"
                     /translation="MSIMLGTVFFALIQNSIDYGFRTGIYIASGVIASDIILIIISLF
                     GASLIPEGGKVEFVIRAIGAVFLMTYGLLNLRKKKEVQFPETTEQHKFQYFALGFVLN
                     IINPANLFWWIAVSANLKAVAAYSTVETGLYYTGALSGIFGVEVLIAYLATTLKKFIT
                     KRFLRNVDIVVGILFIGFSIALFWPVVNAWLELYR"
     gene            835..1719
                     /locus_tag="PK610_04220"
     CDS             835..1719
                     /locus_tag="PK610_04220"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRJ35165.1"
                     /translation="MQNFNETVLFLITTPLFVIFIGLEIIVSNAQRNHRYSSLGFWEN
                     MYLMIINTGIDLGMRIVALYFFTELLTVRFFEWEYSVIYWILLFVFEDLTFWTIHYVD
                     HYVRLFWAVHVTHHNSQEYNLTVGLRSSLFEPLYRFLYFIPMVLGGFRVEDIFFAYSV
                     TQLYGVFLHTQYVKNFGPLDYLFISPSQHRVHHGSNVRYLDKNMGMFLNIWDKLFGTY
                     EKETEQVVYGITKNIESHDPRTVIFHEFKSIADDVRNAPTFKAKFMYIFGPPGWSHDG
                     SRKTSKQLRKELHFQGNK"
     gene            complement(1735..2157)
                     /locus_tag="PK610_04225"
     CDS             complement(1735..2157)
                     /locus_tag="PK610_04225"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRJ35166.1"
                     /translation="MENHYRFLEQLYYRGPINGLLKQSIEVRADKCVITHEVDPQFFH
                     AGNALHGAMYFKLLDDSAYFACAGREKEFFIVTISFTLDLMRPVDSGKLRAEGCCTGE
                     ENGILQGESILYNEEGKVVAKGNGKFARSKTRIADLAE"
     gene            complement(2164..2817)
                     /locus_tag="PK610_04230"
     CDS             complement(2164..2817)
                     /locus_tag="PK610_04230"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRJ35167.1"
                     /translation="MKRILISTLSAFVLIACGGEQKTETKTGDTLAVEETKAQVCTYT
                     YQHDSTRVEWIAYKFTEKTGVKGRFRYTEVNNTTSSTSMYDVLKGAAISIPIDSLETN
                     DKGRNEKIVKFVFGKMNNTKTITGTLKGFAEDGSSATLVIKMNEIEKEINAPASWEGE
                     KVTLKTDINLGDFNALSSVDAITNACKENHKGSDGIAKVWPDVTIIVSTTLKKECAE"
     gene            complement(2836..3336)
                     /locus_tag="PK610_04235"
     CDS             complement(2836..3336)
                     /locus_tag="PK610_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HRJ35168.1"
                     /translation="MIKVKVNGNKEFSIVPKSNGEGELNGAHYVCSETEVKPGTYHVL
                     YKNGSYTIDVLRHDAESKTMQVMVNGTRYTLELRDKYDELLQSLGMSAGTKTIREMRA
                     PMPGMVIQIMVNAGDTVSKDQPLLILEAMKMENVLKAPGDAVIKSINISKGNAVEKNQ
                     VLISFE"
     gene            3441..6122
                     /locus_tag="PK610_04240"
     CDS             3441..6122
                     /locus_tag="PK610_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRJ35169.1"
                     /translation="MKRIHFLLAVAVLAVSSCKTGTGGTSTTKGGKDPKQSTEKPVTN
                     ATPPKQEEPRQPHYNPSVTRVNDIIHTRLEVSFDYKKTYLHGKATIDVRPYFYPVTQI
                     ELDAKGFDVHKVQMKSAANKDLLFGYDGKVLKITLDRKYTKEETYQLYIEYTAKPNEL
                     DMGGSAAIAGDKGLYFINPDSSEVGKHVEIWTQGETEASSCWFPTIDKPNEKMTHDLF
                     ITVREEFVSLSNGVLIASKKHGNGMRTDHWKMAQPHAPYLVMMTVGDYAVVRDKWRKP
                     SGQEIDVTYYVEREYEQYARDIFGKTPKMLDYFSRILGVEYPWDKYAQVVVRDYVSGA
                     MENTTATIHGEFLYRTRRELLDGHNESIIAHELFHHWFGDLVTCESWANLPLNESFAN
                     YSQYLWDEYEHGEDEADHHAFTEMSGYIMSSRQGGYVDMVRYNYHDKEEMFDAHSYNK
                     GGRILHMLRGIVGDAAFFESLKVYLTENKFQPAEMHQLRLAFEKVTGQDLNWYFNQWF
                     FAKGHPILKITQEYSEQKKMVSVIVEQKQNLDQVPLYVLPVDIDVYTATGKTTHSVKV
                     ENRIDTFRIEVSGTPLLVNFDAQKRLLGEKDDIKPIEQWIHQYKNAPRYLDRREAIEE
                     CGKSNNEEAHKLLVEALNDKSPFLQELAMRNMKKAIKTQKDVIKSSLSEIALNNKGKS
                     VRATAIRQLGRNFPEDASLIPIYEKAVNDSSYNVMSAGLESISKTDGKKAMEMARKYE
                     SEKNSEIKMTVAEIYSEHGTAAEHEFFLSTYKALTGFSRYEFISIYGNYIRRQNDTEV
                     EKGLALFEDVARNGSPWWLKFSGYQQLSMLQSHYSKKELEMTTLAESLRKEGKTMDAD
                     QAQADAVRAKNMSEKIVALINDIKSKETDKNILQYVK"
     gene            6200..6559
                     /locus_tag="PK610_04245"
     CDS             6200..6559
                     /locus_tag="PK610_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35170.1"
                     /translation="MKKLAIISVAMGAAFAVLALYVQFSLAPSADALEALENLEFISG
                     MQSGEEIGPSPTRMLWFEAHEKKMNFSYIVLFGGALTALLGLVAGIKKEKLGWIGFLL
                     GLTAFLIGAMHGTHIFS"
     gene            complement(6566..6919)
                     /locus_tag="PK610_04250"
     CDS             complement(6566..6919)
                     /locus_tag="PK610_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRJ35171.1"
                     /translation="MSKSIQIDRAIGNYLRVLRATKNLSQENLADELKLSVAAYSNLE
                     RGKTEFTIGRIMQIARIFKIDWKELIDAAIQGNDQKKNPLYHLEEEVKQLREEMSAIR
                     NSINKTTPRPYKKKK"
     gene            7062..7550
                     /locus_tag="PK610_04255"
     CDS             7062..7550
                     /locus_tag="PK610_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35172.1"
                     /translation="MISKFKWSFSALVLIVTLSSCKKDKYGDADIISSNELVLNNWVQ
                     LYNDGVNYEHQASVSWDAINQAAVDKGALTGYLKDAGVWVALPYGYADEDFGVNINFM
                     FSSGNIKVNASGYSFTGSVPASTFNGYAVRFVLVTPNVKAANPDVDWSNYEMVSARFG
                     LK"
     gene            7556..7903
                     /locus_tag="PK610_04260"
     CDS             7556..7903
                     /locus_tag="PK610_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35173.1"
                     /translation="MKNILFFFLVLTSCKATYYSGSYGQLNQTQVVLSTSNFKHLGTF
                     SGISSIKKMKLSIKDREGLISEARKNLLQNAKSAGVELTGSRTLVNVSVDMIQNPKRV
                     VVTVTGDIIEFLK"
BASE COUNT         2271 a         1598 c         1741 g         2445 t
ORIGIN      
        1 ctgatgaaat tctattgctg acggagaacg caattcggca cattgaagaa cgttttcttt
       61 ttatcaattc actagaagtt ttgtggtatt tcctcaatga aaataactgt acagtcttta
      121 cctgtataat tcaagccagg catttactac cggccagaaa agcgcaatcg agaaaccaat
      181 aaacaggatc cctaccacaa tatcgacatt gcgcagaaag cgttttgtga tgaatttttt
      241 gagtgtggtg gctaaatatg caatcagcac ttctacgcca aatattccgc ttagtgctcc
      301 ggtatagtac aaaccagttt caacggtgga ataagcggca acggctttca ggtttgcgct
      361 tactgctatc caccagaata aattagcagg attgatgata ttcaatacaa aacctaaagc
      421 gaaatattga aatttgtgtt gttcggtggt ttccggaaat tgcacttctt ttttcttgcg
      481 aagatttagc agtccgtatg tcattaaaaa cacagctcca attgcacgta ttacaaattc
      541 tactttaccc ccttcaggaa ttaaactagc gccgaatagt gaaatgataa tgaggatgat
      601 gtcggatgca atgactccgc tggcaatata gattccggtt cgaaagccgt aatcgatgga
      661 attttggatg agggcaaaaa aaactgtccc aagcataatg ctcatcacca ttccggtgcc
      721 taatcctatc agaagagctt ccagggtaga atttttctgc taagctaagg cttttctgca
      781 ttcatgctga attcatattg cctttgattt gtttaccttt tgtacatttg tgctatgcag
      841 aattttaacg agaccgttct atttctgatc accactccgc tgtttgtgat atttatcgga
      901 ttggagatca ttgtctcgaa tgcgcagcgc aatcaccgct attcttcgct tgggttttgg
      961 gagaacatgt atctcatgat catcaatacc ggaattgatc tggggatgag gattgtcgcg
     1021 ctttactttt ttacggagct gcttactgtg cgtttttttg aatgggagta ttcggttatc
     1081 tactggattt tgctctttgt attcgaggat ctgacttttt ggaccattca ctatgtcgat
     1141 cattatgtcc gtttattctg ggcggttcat gtaacgcatc acaattcgca ggaatacaat
     1201 ttaacggttg gattacgctc ttccttgttt gagccccttt atcgttttct ttattttatc
     1261 ccgatggtgc ttggtggttt tcgagtggag gatatttttt tcgcctattc ggtaactcag
     1321 ctttacggtg tttttctgca tacacagtac gtaaagaatt tcggacctct cgattatctt
     1381 tttatttccc cttcacaaca ccgtgtgcat catggaagca atgtgcgtta ccttgataag
     1441 aatatgggga tgtttctcaa tatctgggac aagctattcg gcacctacga gaaagaaaca
     1501 gaacaggtgg tttacggtat tacgaaaaac atcgaatcac acgatccgcg aacagtaatt
     1561 tttcatgaat tcaaaagcat cgctgatgat gttagaaatg caccaacatt caaggcaaaa
     1621 ttcatgtaca tcttcggccc tccgggatgg agtcatgatg gtagcagaaa gacatcaaaa
     1681 caattgcgca aagagttgca cttccaagga aataagtgat tgcagctttt acggttattc
     1741 agccaagtca gcaattctcg ttttcgatcg tgcaaatttt ccgtttcctt ttgcaactac
     1801 ttttccttct tcattgtaaa gtatcgactc tccttgtaga attccgtttt cttctccggt
     1861 gcaacatcct tctgcgcgaa gttttcctga gtcaaccgga cgcattagat ccagcgtaaa
     1921 tgaaatggtc acaataaaaa attccttttc tcttccagca caggcaaaat aggcggaatc
     1981 atccagcagt ttaaaataca tggcgccgtg caatgcattt cctgcatgaa aaaattgggg
     2041 atctacttcg tgagtaatga cacatttgtc agcacgtact tcaatgctct gttttaaaag
     2101 tccgttgatc ggaccacgat aatagagttg ttcgaggaaa cggtaatgat tttccacctt
     2161 caattattcc gcacactctt ttttgagcgt agtagaaacg atgatggtta catccggcca
     2221 aacctttgca atgccgtctg atcccttgtg gttttcttta caggcattgg taatggcatc
     2281 aacagaggat agcgcattga aatcgcccag attgatatct gttttcaatg taactttttc
     2341 gccttcccag cttgccggag cgttgatctc cttttcgatt tcattcatct tgatgacaag
     2401 cgttgcgctt gaaccatctt cagcaaatcc ctttaatgtg ccggtgattg tttttgtatt
     2461 gttcatttta ccaaaaacaa attttacgat tttttcattc ctgcccttat cgttggtttc
     2521 aagactatcg ataggaatgg aaattgccgc acctttaagc acatcgtaca tggaagtaga
     2581 gctcgtggta ttgtttacct cggtgtaacg aaagcgtccc ttaactccgg ttttttcggt
     2641 gaacttatag gcgatccatt ctacgcgtgt agaatcgtgc tggtaagtgt aggtacacac
     2701 ctgcgctttg gtttcttcaa ccgcgagtgt gtcgccggtt tttgtttctg ttttttgttc
     2761 gcctccgcaa gcgataagta caaatgcaga taaagtagag ataaggatcc gtttcataat
     2821 cttgaaaaat tgggtttatt cgaatgagat gagtacctgg ttcttttcaa cagcgttgcc
     2881 cttcgaaatg tttatcgatt ttataacagc gtcacccgga gctttcaata cgttttccat
     2941 tttcatcgct tcgaggataa gtagtggttg atctttgctt accgtgtcgc ccgcattcac
     3001 catgatctgg attaccattc cgggcattgg agctctcatc tcacggatgg tttttgttcc
     3061 ggcactcatt ccaagggatt gaagcaactc gtcgtactta tccctcaact caagcgtata
     3121 gcgtgtgcca ttcaccataa cctgcatggt tttgctttct gcatcatgtc gaagcacatc
     3181 gattgtgtag cttccgtttt tgtaaagcac atgataggtt ccgggcttca cttctgtttc
     3241 gctgcagaca tagtgcgcac cgttgagctc tccttcgccg tttgactttg gaacaatgct
     3301 gaactccttg ttgccgttga cttttacctt gatcaccgtt gatacttgtt ttataacgct
     3361 aaactaaaca ttttgccaca tatacaaaga aagttccccg tatgggtgaa aatcggatat
     3421 ttgcgaacta actgattatc atgaaaagaa tacattttct acttgctgtt gcagtactgg
     3481 ccgtgtcatc ctgcaagacc ggtaccggtg gaacatctac caccaaaggg ggcaaagatc
     3541 ccaagcaaag caccgaaaag ccggtaacaa atgcgactcc accaaagcag gaagagccac
     3601 gtcaaccaca ttataaccca tcggttacac gagttaatga tatcattcac acccgactcg
     3661 aagtgagctt cgattacaaa aagacatatc tgcatggaaa agcaaccatc gatgtgcgtc
     3721 cgtattttta tccggtaacc caaatcgagc ttgatgccaa gggtttcgat gtccataagg
     3781 tacaaatgaa gagtgctgcc aataaggatt tgttgtttgg ttacgatgga aaagttttga
     3841 aaattactct cgatcgcaaa tacacgaaag aagaaacgta tcagctttat atcgaataca
     3901 ctgcaaaacc aaacgaactc gatatgggag gcagtgctgc aattgcgggc gacaaggggc
     3961 tctattttat caatcccgac agcagtgagg taggcaagca tgtagagatc tggacacaag
     4021 gcgaaactga agcaagttcc tgttggtttc ctacaatcga caagcccaat gaaaaaatga
     4081 cacacgatct tttcattacc gtgcgcgaag aatttgtttc cctctccaat ggagttttga
     4141 tcgcctccaa aaaacacggt aacggaatgc ggaccgacca ttggaaaatg gcacaaccac
     4201 acgctccata tttggtgatg atgacggtgg gagattatgc tgttgttcgt gataaatgga
     4261 gaaagccaag cggtcaggaa attgatgtga cgtattacgt ggaacgcgag tacgaacaat
     4321 atgcgcgcga tatttttggg aaaacaccga aaatgctcga ctatttttca cgaatactgg
     4381 gggtagaata cccatgggac aaatacgcac aggtggtggt acgcgattat gtatccggtg
     4441 cgatggaaaa tacaacagca actattcatg gtgaattttt gtaccgtaca cgtcgcgaat
     4501 tgttggatgg acataacgag tcgattattg cacacgaatt gtttcaccac tggtttggag
     4561 atttggtaac ctgcgaatca tgggctaatc taccgctcaa cgaatcgttt gccaattaca
     4621 gtcaatacct ctgggacgaa tacgagcacg gtgaagatga agcggatcat catgccttta
     4681 cagaaatgag tggttatatc atgagctctc gtcagggtgg ttatgtagac atggttcgct
     4741 ataattacca cgacaaggaa gagatgtttg atgcacactc ctacaacaaa ggagggcgca
     4801 tccttcatat gttacgtggt atcgttggtg atgctgcatt ttttgaatcg ctgaaagttt
     4861 accttaccga aaacaaattt caacctgccg aaatgcatca gttgcgtttg gcttttgaaa
     4921 aagttaccgg tcaggatttg aactggtatt tcaatcagtg gttttttgcc aagggtcatc
     4981 cgatactgaa gattactcag gaatattccg agcaaaagaa aatggtatct gttattgttg
     5041 aacagaaaca gaatctcgat caggttccgc tttatgtatt acctgttgat atcgatgtat
     5101 acactgcaac cggaaaaacc acgcactcgg taaaagttga aaacagaata gatacgttta
     5161 gaattgaagt tagcggaaca cctcttcttg taaattttga tgcgcaaaaa cgcttattgg
     5221 gagagaaaga tgatattaag cctattgagc agtggattca ccaatataaa aatgcgccaa
     5281 ggtacctcga ccgccgcgaa gctattgagg agtgtggaaa gagcaacaat gaggaagcgc
     5341 ataaattatt ggtggaagca cttaatgata aatcgccctt tttgcaggaa cttgccatgc
     5401 gtaatatgaa gaaggcaatt aaaacacaaa aagatgtgat caaatcgagt ctgtctgaaa
     5461 ttgcacttaa caacaaaggg aagtcggtac gtgcaactgc aatacgtcag ttgggaagaa
     5521 atttcccgga agatgcaagt cttattccta tttatgaaaa agcggtgaac gattcttcgt
     5581 acaatgttat gtctgccggt ttggaaagta tttcaaaaac agacggcaaa aaagccatgg
     5641 aaatggcacg taagtacgaa agcgaaaaga acagcgagat aaaaatgaca gtggcagaaa
     5701 tttactctga acatggtact gctgccgagc atgaattttt ccttagcaca tataaagcac
     5761 tcacaggatt tagccgctat gaattcattt ctatttacgg aaattatatt cgtcgccaaa
     5821 acgataccga agtagaaaag ggtctggcac ttttcgaaga tgttgcacgc aacggaagtc
     5881 cctggtggtt gaagttttcg gggtatcagc agctttcaat gttgcagtcg cattattcaa
     5941 aaaaggagct cgaaatgact acccttgctg aaagtttacg caaggaaggc aaaacaatgg
     6001 acgctgacca ggcgcaggca gatgctgtac gggcaaaaaa catgagtgag aaaattgttg
     6061 cgctgataaa tgacattaaa tccaaagaga ccgacaagaa tatccttcaa tatgtgaagt
     6121 gaggaatttc ttagtgaatc ttagtggcct tgtgtcttag tggtttattt tctaaattgt
     6181 agtccaaaac caaacaacca tgaagaaact agccattatt tctgttgcaa tgggcgctgc
     6241 atttgctgtg cttgcattgt atgttcaatt tagtcttgcc ccttctgccg atgctcttga
     6301 ggcgctcgaa aacctcgagt ttatttcggg catgcaaagc ggagaggaaa tcggtccaag
     6361 cccaacccgc atgctttggt ttgaggctca tgaaaagaaa atgaatttta gctacatcgt
     6421 tctttttggc ggagcactga cggccttgct tggacttgtt gccgggatta aaaaagaaaa
     6481 gttgggatgg atcgggttcc tacttggttt gaccgccttt ttgattggag ccatgcacgg
     6541 tacgcacatt ttctcttaag agtttttatt tctttttctt gtagggtctg ggtgttgttt
     6601 tattgatgct attacgaatc gcggacatct cctcccttaa ttgtttaacc tcctcttcca
     6661 aatggtagag ggggtttttc ttttggtcat ttccctgtat tgcggcatca attaattctt
     6721 tccaatcgat tttgaatatt ctggctattt gcatgatccg acctatagta aattctgttt
     6781 tacctcgttc cagattcgaa taggcagcta cggatagttt aagttcgtct gccaggtttt
     6841 cctggctcag gttttttgtt gcccttaata cacgcaggta gtttccaatt gcccggtcga
     6901 tctgaatgga tttgctcata atgcaaagtt ccattaagct tgggattcag atgtttataa
     6961 taaataaaag aattttagta tttatagaat tattttatat ttgctgtatg gcgacccccg
     7021 aaaagcccaa atagagtagg gagataatta atttttattg tatgatttca aaatttaaat
     7081 ggtctttttc tgctcttgta ctgattgtga cactttcctc ttgtaaaaag gataagtacg
     7141 gtgacgcaga cattatttct tccaatgaat tggtattgaa taattgggtt caactttaca
     7201 atgatggtgt taattatgag catcaggcct cagtttcatg ggatgctatc aatcaggctg
     7261 cagttgacaa gggagcattg acaggatatt taaaagacgc aggtgtttgg gttgcattac
     7321 catatggcta tgcggatgaa gattttggag taaacatcaa ttttatgttc agttcaggaa
     7381 acattaaggt gaatgcatcg ggatattcct tcactggctc agtaccggca tctacattta
     7441 atggatatgc agtaaggttc gtattagtca cgcccaatgt taaagccgct aacccggatg
     7501 tggattggag caattacgaa atggtttctg cccgctttgg attaaagtaa tatccatgaa
     7561 aaacatccta ttcttttttt tagttcttac atcatgtaag gctacatact attcagggtc
     7621 ttatggtcag ttaaaccaaa ctcaggtcgt actatctacg tcgaatttca aacaccttgg
     7681 tacttttagt ggaatctcat ccattaaaaa gatgaagctg agtattaagg atcgggaagg
     7741 attaatctct gaggcccgga agaatcttct tcagaatgca aaatctgccg gcgtagagct
     7801 aacgggtagt cgaacgcttg ttaatgttag tgtagatatg attcagaatc ccaagcgagt
     7861 ggtggttact gttaccggag atataattga gtttttgaag taaatccaat agggatttat
     7921 tctattgtct gctaaaacaa atgaaaacaa tgaagaaaat tttacttttt gctttgactc
     7981 tggcactaat tgatttgatt ttaatcataa ccggcgacct taaaccaaaa gaaggagagt
     8041 atgataagac ttttt
//
LOCUS       DAOMYG010000090         8012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_2991_length_8012_cov_8.457710, whole genome shotgun sequence.
ACCESSION   DAOMYG010000090 DAOMYG010000000
VERSION     DAOMYG010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8012
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2991_length_8012_cov_8.457710"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PK610_04265"
     CDS             <1..416
                     /locus_tag="PK610_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRJ35174.1"
                     /translation="NLLNNNFGNCSLIDTVFYKSNIEKCDFSLSDFSGAEITETNMEN
                     NMVNDVVWKFTKFSRTNISHMIFTGTIEDCHFEHCSFYGVKFENATILNTFFKYNERF
                     KKVLFVNCKVDKITYAFLKNNSANLEGISIIEFSE"
     gene            complement(479..1579)
                     /locus_tag="PK610_04270"
     CDS             complement(479..1579)
                     /locus_tag="PK610_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019976715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRJ35175.1"
                     /translation="MRLFFGFIVLFCSALQLNAGSDSLQTGKTNGGLVIDGFADVYYA
                     FDFNQPASGNRQPFMFNHNRHNEVNLNLGFVRVGLEKENYRAKLALHTGTYVNANYAA
                     EPGLLKSLFEGNIGFSLNKKRTVWIDAGILPSHIGFESAVSLDNTTLTRSLLAENSPY
                     FMSGIKLTYNNDKGLEISGMLLNGWQRIQRVPGNSLPSFGTQITYSPTDKFSFNWSSF
                     TGTDDPDSTRRLRVFNNFYSKIQLSKKLGIIAGFDIGMQQSQKHASNYHMWLSPVLIA
                     QVKLHPKWNSALRLEYYHDENGVMIYTGTPNGFQTSGVSLNFDYVRSENVMWRIEGRM
                     LNARDEIFERNGSPVRNNFFITTSVAVRFGGE"
     gene            1915..2304
                     /locus_tag="PK610_04275"
     CDS             1915..2304
                     /locus_tag="PK610_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HRJ35176.1"
                     /translation="MFTAEHIKNAHSKVKSGADFPMYIKEIKAMGVTRYETFVKDGRI
                     DFFGADDHCVQIPEKYNSITIADHANTEGFKSGLLDHQQGKTDYPTFIAMCANTGIDK
                     WKICMNKMTCTYFDKSGNIVLEEKIPQ"
     gene            complement(2641..5274)
                     /gene="alaS"
                     /locus_tag="PK610_04280"
     CDS             complement(2641..5274)
                     /gene="alaS"
                     /locus_tag="PK610_04280"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009388981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRJ35177.1"
                     /translation="MTAAEIRKTFLEYFRSKQHEIVPSSPMVVKNDPTLMFTNAGMNQ
                     FKDLFLGNSPIKHPRIADTQKCLRVSGKHNDLEEVGVDTYHHTMFEMLGNWSFGDYFK
                     KEAIEWSWELLTEVYKIDKDRMYVTVFEGSKDDGLGFDQEAYDHWKKFIAEDRILKGN
                     KKDNFWEMGDTGPCGPCSEIHVDCRPDAERAKGDGASLVNNDHPQVIEIWNNVFMEFN
                     RKADGSLEKLPAQHVDTGMGFERLVRVLQGKSSNYDTDVFMPLIRKIENLSRKKYNGA
                     DTSDAQEKINIAMRVIADHIRAISFSIADGQLPSNNGAGYVIRRILRRAIRYGYQSLE
                     MRDPFFFQLVAVLAAEMGDAFPELRSQKELIEKVIREEEVSFYRTLENGIRRIENVCE
                     ELKSGKEKVIDGSTVFELYDTFGFPVDLTSLIARGHGLSIDESGFQKELEKQKERSRA
                     ATSIETDDWVVLREDQEEEFIGYDHLETNISIVRYRKVKQKGKELFQLVFDYTPFYPE
                     GGGQVGDKGYLQSGNEKVFIIDTKKENNLIVHFSEKLPADPSSQFIGVVDRSKRKDTA
                     CNHSATHLLHEALRDILGTHVEQKGSLVNSEYLRFDFSHFSKMSDEEIRNVEKQVNER
                     IRENLQLDEHRAMPVDDAKAMGAMALFGEKYGDKVRVIRFGSSVELCGGTHVRSTGEI
                     GMFKIVSEGAVAAGIRRIEAITGTKADEHYRKQEDLLNQVSDLLKRPADVVKALTDLV
                     QQNAELNKKMELLAKEKAGSIKSELLKKVRSVNGVNIIAQKVDLDTANIKDIAYQLKG
                     EVKSLFLLLANEQDGKPGITVMIDESLVNERKLHAGNIVRELAKEIKGGGGGQPFFAT
                     AGGTDASGIARVLERVEELVG"
     gene            5615..6592
                     /locus_tag="PK610_04285"
     CDS             5615..6592
                     /locus_tag="PK610_04285"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRJ35178.1"
                     /translation="MTKVKYKYNPQTLTYEKVEPGIRDRIRKALPYLAGSIVFAVAII
                     LIYENQSFFKSPREQAFERENEQLRQQYDLLKKQMREMEIVLSDIQLRDDNIYRVIFE
                     SEPYPNHKRDRATGGSNKYRQFAEYESGQLMTETWQALDRIEKKLYAQSKSFDEVIKL
                     AKTKEKMLSSIPAIIPVNRKDLRAGIGPYGWRIDPIYKTRAMHTGMDFPSETGTPVYA
                     TGDGKVINVESNYWGYGNIVLIDHGFGYQTMYAHLSAFKAKIGQVVKRGEVIGLVGST
                     GKSTAPHLHYEVLRNGEKVNPVNYYYNDLSPAEYEEMLKAASSSGTSFD"
     gene            6601..6957
                     /locus_tag="PK610_04290"
     CDS             6601..6957
                     /locus_tag="PK610_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRJ35179.1"
                     /translation="MPYKERPTQKLYYSIGEVAAMFGVNTSLIRFWEKEFDVIKPTKN
                     KKGNRLFTAEDIENFKKIFTLVKGEGYTLQGAKEKIRELDHKEEELINDELDKRAVIE
                     KLIMLKDKLRQIKDQL"
     gene            complement(6948..>8012)
                     /locus_tag="PK610_04295"
     CDS             complement(6948..>8012)
                     /locus_tag="PK610_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35180.1"
                     /translation="KLFFRGGSRRDQQLLQYDFESNTLKTIPLPGNVQDAKFLFYVPD
                     GSSSILHVALSAGKGKGFKFYSHFFNENGEFAKKSQRVIEEPGYYCRTASMTELSENE
                     YILVGTYSKGKAVTTNGVYFARYENDNQVVNKRYSFSDFPEFSKKVGDGRTGFNNWGN
                     VERQTNRKTQKGKTDFLEANVLIHSLFKFQGNYVYIIECYYPTFRTEYSSMTNQNAST
                     ARSVFDGNVFTHALVLGLDHLGNVVYDFTFDLPKKLLKDYQARRQLGSIRKDDAVDIY
                     FKYPKDLECMRLSNNQLTEIKKVRTDSDSLDAVYSAKSQHWYGDYYIRYEMIKRLIPG
                     EKKKEETIFETVKYKKEIHN"
BASE COUNT         2431 a         1870 c         1511 g         2200 t
ORIGIN      
        1 ccaatctgct caacaacaat tttggtaatt gttcgctcat tgatacagtg ttttacaaaa
       61 gcaatataga aaaatgtgac ttttcattat ctgatttttc cggagcagaa attacagaaa
      121 cgaacatgga aaacaacatg gtaaatgatg ttgtctggaa attcaccaaa ttttcccgca
      181 caaatatcag ccatatgatt ttcaccggca caatagaaga ttgtcatttt gaacactgtt
      241 cattctacgg agtgaaattt gagaacgcaa caatccttaa tacctttttt aaatacaacg
      301 aacgatttaa gaaagttctg tttgtaaact gtaaagtgga taaaataact tatgcattct
      361 taaaaaacaa tagtgctaat ttggaaggca tttcaattat agaattcagt gaatagtgaa
      421 cggtgaaaag tgaacagcag caactcctaa cttataactc ctaactttta cccgctaact
      481 actctccccc gaaccgaaca gctactgaag tcgtgataaa gaaattgttc cgcacaggtg
      541 atccatttct ctcaaaaatt tcatcacgcg cattgagcat tcgtccttcg attcgccaca
      601 tcacgttttc gctgcgcaca taatcgaaat tcagggaaac acctgaagtc tgaaaaccat
      661 tgggtgttcc cgtataaatc attacaccgt tctcatcatg gtaatattcc aatcgcaggg
      721 cagaattcca tttcgggtgt agttttacct gcgcaatcag aacaggactc agccacatat
      781 gataattgga agcatgtttt tgactttgct gcattccgat atcaaatcct gcaatgattc
      841 ccaacttttt cgaaagctga attttgctgt aaaaattatt gaacaccctt aaacgtcgcg
      901 tactgtcggg atcatcagta ccggtgaaac tgctccaatt gaacgaaaat ttatctgtcg
      961 gcgaatacgt gatttgtgta ccgaaagaag gcagtgagtt accgggaaca cgctgaatgc
     1021 gctgccaacc gtttaaaagc attccggaaa tttccaatcc tttatcgttg ttgtacgtta
     1081 gctttattcc cgacataaaa tacggtgaat tctcagccaa tagagacctt gtaagtgtgg
     1141 tattgtctaa ggaaaccgcc gactcaaatc cgatgtgtga aggcaaaata cctgcatcga
     1201 tccatactgt tctcttttta ttcagcgaaa agccaatatt cccttcgaac aaacttttca
     1261 gcaaaccggg ttcagcggca taattcgcgt tgacataagt accggtgtgc aatgcaagtt
     1321 ttgcgcggta attttctttc tccaacccaa cacgaacaaa accaaggttc agattgacct
     1381 cgttatgtcg gttgtgattg aacataaaag gctgtcggtt cccggaagca ggctgattaa
     1441 aatcgaaggc ataatacaca tccgcaaatc catcgatcac aaggccacca ttcgtcttgc
     1501 cggtctgcaa actatctgat cctgcattca actgaagtgc ggaacaaaac agaacgataa
     1561 aaccaaaaaa taatcgcata cggttcagac ttcaacttta caggtagcag gcactactga
     1621 attggacatt caaaactacg acaaaatttc aattgaaatt gcatgatagt tgtcaggaaa
     1681 acacaccaaa aacccgagca atagtgggac ttccggcttg ataattagga ttacgcattc
     1741 cactatagaa accctgagtc ccgccttgag aaacaaacaa taaaaatgca gaacacaagg
     1801 agtactcaca agttcatctc aaatgctttt ccctcattca ctcattgcat tcacagtagt
     1861 tttgagtatt ttcagttttc aaattccgca taaaatcatc taaaccaaat tctaatgttt
     1921 accgcagaac acatcaaaaa cgctcacagc aaagtgaaat caggtgcaga tttcccgatg
     1981 tatatcaagg aaataaaagc aatgggtgtg accaggtacg agacctttgt taaggatggt
     2041 cgaattgatt ttttcggagc tgatgatcat tgcgttcaaa ttcctgaaaa atacaattcc
     2101 attacaattg cagaccatgc caacacagaa ggattcaaat caggactact agaccatcag
     2161 cagggaaaaa ccgattaccc gacattcatt gctatgtgtg cgaataccgg aattgacaaa
     2221 tggaaaatct gcatgaataa aatgacctgt acttatttcg ataaatccgg aaacattgtt
     2281 ttggaagaga aaataccgca ataatttcag gatagaatgc gggtgaacgg tgatcagtga
     2341 tcccgacgcc attggtcggg acaggccagt gatcagtgaa cggtaattgg tgaacagaaa
     2401 acaaacaatt tgccgcaaag taaagattat tgatgggtgg ataatttcgg attcgcagtc
     2461 accccgatga tcacggcttc cggaagatct tttcaaaagg tattccagca atactttcta
     2521 cacaagatga attatccatt caccaccaca acctaagcgt cagtactggc atactagcac
     2581 atgattcgac cgaaggaaga atcatcccga ccgactgaag ggagcgtcgg gattagcaca
     2641 ttaccccact aactcctcca ctctctctaa cacccgcgca atcccactcg catcagtgcc
     2701 acctgcagtt gcaaagaaag gctgaccacc gccaccgcct ttgatttctt ttgcaagctc
     2761 gcgcacaata tttcccgcat gcagttttcg ctcgttcacc agactctcat caatcatcac
     2821 cgtaataccc ggtttaccat cctgctcatt ggcgagtaag agaaacaatg attttacttc
     2881 gccttttaac tgataggcga tgtcttttat attggccgta tcgagatcta ctttttgcgc
     2941 aataatattt acaccattca ccgaacgtac ctttttaagc aattcggatt ttatgcttcc
     3001 tgctttttct ttcgccagca gttccatttt tttattcagc tctgcatttt gctgaacaag
     3061 atcggtcagt gcttttacca catcggccgg acgcttcaac aaatcgctta cctgattcaa
     3121 taagtcttcc tgtttgcggt aatgctcgtc agcttttgtt ccggtaatcg cttcgatacg
     3181 acgaattcct gcagctacag caccttccga aacaatttta aacataccga tttcacctgt
     3241 agaacgaaca tgtgttccac cacacaattc aacgctcgaa ccaaaacgga tcacgcgtac
     3301 tttgtcgcca tatttttctc cgaacaaggc cattgcaccc attgctttcg catcatcaac
     3361 aggcattgca cggtgttcgt ccaactgaag attttcgcgg atgcgttcat tcacctgctt
     3421 ttctacattg cgaatttctt catcgctcat tttcgaaaag tgagaaaagt cgaaacgcag
     3481 atattccgaa ttcacaagtg aacctttttg ttcaacatgt gtccccagaa tatcgcgcag
     3541 tgcttcatgc agcaaatgtg ttgccgagtg attgcaggca gtatctttcc gcttgctgcg
     3601 atctaccaca ccaatgaatt gcgaagaagg atctgcaggt aatttttcag aaaagtgaac
     3661 tatgagattg ttctccttct tcgtatcgat gatgaatact ttctcgtttc cactttgtaa
     3721 atatcctttg tcgccaactt gtccgccacc ttcaggatag aaaggcgtat aatcgaaaac
     3781 aagctgaaac aattcttttc ctttctgttt cactttgcgg taacgcacaa tggaaatgtt
     3841 ggtttcaaga tgatcgtacc caatgaactc ttcctcctga tcttcgcgca aaacaaccca
     3901 atcatcggtt tcgatactgg ttgctgcacg cgaacgttct ttttgctttt ccagttcctt
     3961 ctgaaatccg gactcatcaa tactcaatcc atgtccacga gcaatgagcg atgttaaatc
     4021 gaccggaaat ccaaacgtat cgtacaattc gaaaacggta gatccatcga tcactttttc
     4081 ttttcctgat ttcaattctt cacacacatt ctcgatacgg cgaatcccgt tttccagtgt
     4141 gcggtagaat gaaacctctt cttcgcgaat cactttttcg atcagctctt tctgtgaacg
     4201 caattcagga aatgcatctc ccatttcggc agccagtact gctaccagtt gaaagaaaaa
     4261 aggatcgcgc atttcgagcg actggtaccc gtaacgaatg gcacgacgaa gaatgcgtcg
     4321 gatgacatat cccgcaccat tgttcgaagg caattgtccg tcggcaatac tgaatgaaat
     4381 cgcgcggata tgatcggcga taacacgcat tgcaatattt attttttcct gcgcatcaga
     4441 agtatcggca ccattgtact ttttacgcga caaattttcg atcttacgaa taagcggcat
     4501 gaaaacatcc gtatcgtaat tggaagattt cccttgcaaa acacgaacaa gacgttcaaa
     4561 ccccattcct gtatcgacat gttgagcagg caatttctcc aatgaaccat cagccttgcg
     4621 gttgaattcc atgaacacat tgttccagat ttcaatcacc tgcggatgat cgttattcac
     4681 cagcgaagca ccgtcacctt tggcgcgttc tgcatcggga cggcaatcga catgaatctc
     4741 actgcaaggg ccacagggac cggtatcacc catttcccag aaattatctt ttttattccc
     4801 tttgaggatg cggtcttctg caataaattt tttccaatga tcatacgctt cctgatcgaa
     4861 acccaaacca tcgtccttgc ttccttcgaa tacggtgacg tacatacgat ctttatcgat
     4921 cttgtacact tctgtcagta attcccatga ccattcgatc gcctccttct tgaaataatc
     4981 gccgaaactc cagtttccca acatttcgaa catggtatgg tgataggtat ccacacccac
     5041 ttcttcgagg tcgttgtgtt taccggaaac gcgcaaacat ttttgagtat ctgcaatacg
     5101 cggatgtttt attggagaat taccaagaaa aagatccttg aactgattca ttcccgcgtt
     5161 ggtaaacatg agtgtaggat cattctttac caccattggt gaggaaggaa caatttcgtg
     5221 ttgtttcgac cggaaatatt ccaggaaggt tttgcgtatt tcggcagctg tcatcatagg
     5281 gaaaaacttg cgaggcgcaa atgtaggaaa acagccccat cacaagggtg aaaaagggga
     5341 ataaaatgag gtaagagaat gggaacggga acggagacta ggacgaggac gaggacaatt
     5401 ataattacct attaccaatt acccagacac caaattaccc tatacccaat taccaattta
     5461 ccaattacct aattacccag acacccaacc accccctccc ccttgccaat caacgtctta
     5521 catcatcaat cccaacccaa taactccaaa cataaaacga atatctcccc cctcttccct
     5581 tccatttccc gctgaatcgt taacttagac gctcatgacc aaggtcaaat acaaatacaa
     5641 tccgcagacc ctcacctacg aaaaggtaga accagggatt cgcgaccgga tcaggaaagc
     5701 acttccttat ctggcgggaa gtattgtttt tgctgttgcg atcattctga tttacgaaaa
     5761 tcaatccttt ttcaaatcac cccgcgagca ggcctttgag cgggagaacg aacaactgcg
     5821 tcagcaatac gatctcctga aaaagcaaat gcgcgaaatg gaaattgtgc tctccgatat
     5881 tcaactgcgc gacgataaca tttaccgcgt tattttcgaa tcggaaccct atcccaacca
     5941 caaacgcgat cgtgcaaccg gcggaagcaa caaataccgt cagttcgccg aatacgaaag
     6001 cggtcagctc atgaccgaaa cctggcaggc gcttgatcgc atagagaaaa aactatacgc
     6061 acaatcgaaa agtttcgatg aagtgattaa actcgccaaa acgaaagaaa agatgctgag
     6121 cagtattcct gctatcatcc ctgtaaaccg aaaagatctc agggcaggaa ttggtcctta
     6181 cggctggcgt atcgatccga tctacaaaac aagagccatg cacaccggaa tggatttccc
     6241 cagcgaaaca ggcacaccgg tatatgcaac aggcgacgga aaagtgatca atgtggaatc
     6301 gaactactgg ggatacggta acattgtatt gatcgatcat ggtttcggtt accagaccat
     6361 gtatgcccac ctcagtgcat ttaaggcgaa aatcggacag gtagtaaaac gcggagaagt
     6421 gatcggactc gtgggtagta ccggaaaatc gacagctccg catttgcatt atgaagtact
     6481 gcgcaatggc gaaaaggtaa atcccgtgaa ttattattac aacgatcttt cgcctgcaga
     6541 atacgaagag atgttgaagg cagcatcttc atccggaacc tccttcgact aatcatcacc
     6601 atgccctaca aggaacgccc cacacaaaaa ctgtactact ccattggaga agttgcagct
     6661 atgtttggcg tgaatacatc actgatccgc ttttgggaaa aggaatttga tgtaatcaaa
     6721 cccacaaaaa acaaaaaagg gaatcgtctc tttactgcgg aagacattga gaatttcaaa
     6781 aaaatattca ccctggtaaa aggcgagggt tataccttac agggcgccaa ggaaaaaata
     6841 agagagttgg atcacaagga agaagaactg atcaatgacg aactcgacaa aagagccgtc
     6901 attgaaaagc tgatcatgct caaagataaa ttacgacaga tcaaagatca gttgtgaatt
     6961 tcttttttgt atttgactgt ctcaaatatt gtttcctctt ttttcttctc accgggaata
     7021 aggcgcttga tcatttcata cctaatgtaa taatccccgt accaatgctg tgattttgct
     7081 gaataaaccg catctagaga atctgaatcg gtcctgactt ttttgatctc tgttaattgg
     7141 ttattggaca aacgcatgca ttctaaatct ttaggatact tgaaataaat atcgacagca
     7201 tcatctttgc gtatacttcc aagttgacgt ctggcttgat aatcttttaa cagctttttg
     7261 ggcaaatcga atgtaaagtc atatacaaca ttccctaaat gatccaatcc aaggactagt
     7321 gcatgggtaa acacattacc atcaaaaact gatcttgcag tacttgcatt ttggttcgtc
     7381 atactactgt attcagttcg gaatgttgga taatagcatt caataatgta aacataatta
     7441 ccctgaaact tgaacaatga atgaatcagg acattggcct ccaaaaaatc cgtttttccc
     7501 ttttgggttt tccgattggt ctggcgctca acatttcccc aattattaaa tccggttctg
     7561 ccatcaccaa cttttttgct gaattcaggg aaatctgaaa aactgtatcg tttattcaca
     7621 acctgattat cgttttcgta acgcgcaaaa taaactccat ttgttgttac agcttttccc
     7681 ttcgaataag tccccacaag gatatattca ttctctgaca attctgtcat tgatgcggta
     7741 cgacaataat agcccggttc ctcaattacc ctttgggatt tttttgcaaa ctccccattc
     7801 tcattaaaaa aatggctgta aaatttaaat cctttacctt ttcctgcaga aagggcaaca
     7861 tgtaaaatag aactggaacc gtctggaaca taaaatagaa actttgcgtc ctgaacatta
     7921 cccggtagag gaattgtctt taaagtgttg ctctcaaaat catactgaag tagttgttga
     7981 tctctgcggg atccaccacg aaaaaataat tt
//
LOCUS       DAOMYG010000091         7957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3014_length_7957_cov_8.653505, whole genome shotgun sequence.
ACCESSION   DAOMYG010000091 DAOMYG010000000
VERSION     DAOMYG010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7957
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3014_length_7957_cov_8.653505"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2629)
                     /locus_tag="PK610_04300"
     CDS             complement(<1..2629)
                     /locus_tag="PK610_04300"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ35181.1"
                     /translation="MLQKPVIHSITIVCLMLCAVINAFSQSKEIDSLRKVSAELSGTK
                     KADAMILLARRMYSFDKEQSVTLFKDAETYCSGIHYKGGLSDIYLAMGKMNRTEKNYP
                     LAMDYFKRAHAAAADPFDPDRVGPAIIGEISVLKAQNKYEEAGKLAEESVAWARTQSS
                     QALLADMLGQVGQIIFQKGDYKSALKIFEEQRLIRNKIGPESEVGGLTMNIGVMHYRM
                     GDLEGSLPYYNFALESYTRQKDTMNLGHVWVNFAMTYNDLGQIPKALECMDKASYYYL
                     AINDMNNYSNIISNSTFIYINAGEYGKAMKMIVPALERFEKNNDKKNMATAYGSMSSV
                     NLHMRDTVKALEYIDRAVALQREIGLSGSLGYSLIGKGNILSAKGQLKEAEICYREAM
                     SIRMKNNDKMGMAGSHLSLGNNFKRMALYDSSLYHYQTALQIYESSGKKQSVAGLYSN
                     IGVVYYDLGEYDKALENYQKAFEIRKDLGLKQDLYETYLTYSNVYKKMGNYEKAYEYY
                     QKYFIEYDSLNNATARKEVLDLQTKYDTDKKQKEIELLSARERMQGLAFEKQNALLMF
                     ERLQLEQKGKEVELLNKDKSLKEAELLREREAKMRKQKEVDALQQENLLKEEINAKQR
                     QMTSIFIGAFVLMLITSGVIFRFYRQNRRAKIIISQQKEEVEQKKNQIELQKELMEEK
                     NREITDSIRYAKRLQDAILPPTGFFRSLLPDSFVFYQPKDIVAGDFYWIEKSDDTIFL
                     AVADCTGHGVPGAMVSIVGHNGLERCVKEFGLKDPEAILDKLNELVEGTFSRSEGQVR
                     DGMDIALCIIKKSGDSTELTYSGANNPLWIIRADNTLEEIKADKQPIGRYDNRKPFTA
                     KKVRLLDGDQIYLFSDGYAD"
     gene            2716..4347
                     /gene="pruA"
                     /locus_tag="PK610_04305"
     CDS             2716..4347
                     /gene="pruA"
                     /locus_tag="PK610_04305"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HRJ35182.1"
                     /translation="MPKGIYNVPAAHNEPVKSYAPGSAERKELQAMLKALRSVKTDIP
                     MYIGGKEVRSGVKSRLAPPHDHQHTLGFYHKSDKKHIKQAIDAALKAKKNWEDLSWEH
                     RASIFLKAAELIAGPYRAKLNAATMLGQSKNAYQAEIDSACEIIDFLRFNVQFMTEIY
                     AQQPVSSPGIWNRMEWRPLEGFVYALTPFNFTAIAGNLPASAAMMGNVVVWKPSNTQV
                     YSANVLMEIFIKAGLPDGVINLIYPSGPDAAEVIFNHADFAGIHFTGSTAVFQNIWQT
                     IGNNIHKYKSYPRIVGETGGKDFILAHPSADPAIVATAISRGAFEFQGQKCSAASRAY
                     VPSNIWPEVKKQVLADLKSMKMGGTEDFTNFINAVIDEKSFDKLTSFIMQAKKDKNAE
                     VIAGGKCDKKKGYFIEPTIIQAKDPHYVTMCEELFGPVLTVYVYKPEKFNETVKLVDT
                     TSIYALTGSIISQDRYAIEYATRNLRNAAGNFYINDKPTGAVVGQQPFGGARGSGTND
                     KAGSMINLLRWVSPRTIKETFVPPVDYRYPFLQKD"
     gene            4469..4864
                     /locus_tag="PK610_04310"
     CDS             4469..4864
                     /locus_tag="PK610_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35183.1"
                     /translation="MKTLFSIVFCCASVALFAQHPQAGKQIHAPTQANTVTPSKDKQP
                     TVNSQVGTKSQASGNNGTVYSEKQVPTENYSSGRTRQTSTPSRSGTATHSASQRNPEY
                     DVEENIPVDIDALQLELRNCNNKIQISVL"
     gene            5087..6049
                     /locus_tag="PK610_04315"
     CDS             5087..6049
                     /locus_tag="PK610_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010134147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HRJ35184.1"
                     /translation="MTKILANDGIDKSGQVLLEKAGFQVITEKVAQDQLANAINENNY
                     EVVLVRSATTVRKEVMDACPNLKMIGRGGVGMDNIDVAYGREKGIDVFNTPGASSQSV
                     AELVFSHLFSMARFLYDANRNMPVKGETEFDKLKKNYAKGIELRGKTIGIIGFGRIGQ
                     SVATYALGCGMKVIAHDPFVKDAHVELEIHGHGKVKVNIKSSSMDELLANADFISLHV
                     PKQKDGSAVIKSAEIEKMKAGVCLVNTARGGVINEDDLIAGLNSGKIAHAALDVFENE
                     PTPRKDLLSHPKISLTPHIGASTVEAQDRIGVEIAEKIISFYKK"
     gene            6059..7303
                     /locus_tag="PK610_04320"
     CDS             6059..7303
                     /locus_tag="PK610_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRJ35185.1"
                     /translation="MAVIRPFKAIRPTRDKAHLVASRSVNLYKQRILNAKLEENPYTF
                     MHVILPEFGSKITTKPNTTERFKLVKKNFEAFRKKGIFIQDKNDCLYIYRQLKDGYSY
                     TGIIAGAAVDDYLNGVIKIHEQTLTKREEIFKNYLDVCGFNAEPVLLSYSDNNTVDKV
                     LKRYTAERSEYEFTTADGVTHFLWIVAEKKDIAIICKAFEKIPAVYIADGHHRSASSA
                     LLAKDRRSKLKKYSGKEMFNYCMAFFISESQLNIYDFNRVVKDLNGLSAKEFLDQLRE
                     KFDVESKGKKCYRPKKLHNFSMYLEGEWYSLTAKKGSFNSKHPVGNLDAQILTDNILS
                     PVLGIHDLKTDSRIEFVGGLQGMEGLQRKVDEGKMKVAFGLFPVKVDQLKQIADTNNI
                     MPPKTTWIEPKLRSGLVIQSLD"
     gene            7320..>7957
                     /locus_tag="PK610_04325"
     CDS             7320..>7957
                     /locus_tag="PK610_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRJ35186.1"
                     /translation="MSISENLHHIESEIPAHVRLVAVSKTKPNEQILEAYAYGQRDFG
                     ENYIQELTAKAEALPKDIRWHAIGHIQTNKVKYIAPFVHLIHGVDSLKLLQEINKQAA
                     KSNRVIDCLLQVFIASEESKFGLDENEVKEILSDEIIRTLPNIRIVGLMGIASNTDDE
                     DQVRKEFRNLKSLFDSVKQLHPGITILSMGMSGDYKMAIEEGSTMIRVGSLI"
BASE COUNT         2410 a         1695 c         1566 g         2286 t
ORIGIN      
        1 gatcggcata tccatcggag aacaggtaaa tctgatcccc atcgagcaaa cgcacttttt
       61 ttgctgtaaa aggtttacga ttatcgtagc gcccaatagg ttgtttatct gctttgattt
      121 cttccaatgt attatcggct cgaatgatcc acaaaggatt atttgctccg gaataagtga
      181 gttctgtgga atcgcccgac tttttaatga tgcataatgc aatatccatc ccgtcacgaa
      241 cctgtccctc actgcgcgaa aaggttcctt ccaccagttc attcaattta tcaagaatgg
      301 cctccggatc ctttaaaccg aactctttca cacaacgctc taaaccgtta tgtccaacaa
      361 tactaaccat tgcaccggga acaccatgtc cggtacagtc tgcaacggct agaaaaatag
      421 tatcgtcgga tttttctatc caataaaaat cgcctgcaac aatatctttc ggctgataaa
      481 aaacaaaaga atcgggcaag agtgagcgaa aaaaacctgt tggtggaaga atagcatcct
      541 gcaagcgttt agcataacga atggaatcgg tgatctcccg gtttttttct tccatgagtt
      601 ctttctgcaa ctcaatctga ttctttttct gttctacttc ttccttttgc tgactgataa
      661 taattttcgc ccttcggttt tgacggtaga agcggaaaat gactcctgaa gtaatcagca
      721 tcaaaacaaa agcaccaata aaaatagaag tcatttgccg ttgtttggca ttgatctcct
      781 ccttcaataa attctcctgt tgcagcgcat ctacttcttt ctgcttccgc atttttgctt
      841 cgcgttcgcg caataattct gcttctttga gcgatttatc tttgttcagt aattcaactt
      901 cttttccttt ttgctcaagt tgcaaacgct caaacattaa taaggcattc tgcttttcaa
      961 aggctagtcc ctgcattctt tctcttgccg acaacaactc aatttctttt tgctttttgt
     1021 cggtatcata tttggtttgc agatcaagta cctcttttcg ggcagtggca ttattcaaag
     1081 aatcgtattc aataaaatac ttctgataat attcataggc tttttcgtag ttgcccattt
     1141 ttttgtagac attagaatac gttaggtagg tttcgtacag atcctgcttt aatcctaaat
     1201 ccttccttat ttcaaatgcc ttttgatagt tttccagtgc tttatcatat tcccccaaat
     1261 cataatacac tacaccaata ttagaataca atcctgcaac actttgcttt tttcctgaac
     1321 tttcataaat ctgtaatgca gtttgataat ggtaaagcga agaatcgtaa agagccatcc
     1381 gcttaaaatt attccctagt gacaaatgag agcctgccat tcccatttta tcattgttct
     1441 tcatccggat actcatggct tctctgtagc aaatctctgc ctcttttaac tgaccttttg
     1501 ccgataaaat attcccttta ccaataagcg aataacccaa agaacccgaa agaccaattt
     1561 cccgttgcaa tgcaaccgcc ctatcaatat attcaagtgc tttaacagta tcccgcatat
     1621 gcaggtttac actcgacatg ctgccatatg cagttgccat gttttttttg tcgttattct
     1681 tttcaaaacg ttccaaagca ggcacaatca ttttcattgc cttcccgtat tcacctgcat
     1741 tgatgtaaat aaacgtggag ttggaaatga tattggaata attattcata tcattaattg
     1801 ccagataata atatgaagct ttatccatac actccaatgc cttcggtatt tgacctagat
     1861 cattgtatgt catcgcaaaa ttcacccaaa catgtccaag gttcatcgtg tctttttgcc
     1921 gggtataaga ttccagagcg aaattatagt agggaagtga tccttccaga tctcccatac
     1981 ggtaatgcat taccccaata ttcatggtaa gccctcccac ttccgattcc ggacctattt
     2041 tatttctgat tagtcgttgc tcttcaaaaa ttttgagggc acttttatag tctccttttt
     2101 gaaagataat ttgtcctacc tgtcccagca tatccgccag taaagcctga ctggattgcg
     2161 ttcttgccca ggctactgat tcttctgcaa gtttaccggc ctcctcgtat ttgttttgtg
     2221 cttttaaaac actgatttca ccaataattg cagggccaac tctatccggg tcaaaaggat
     2281 cagctgctgc agcgtgggcc cttttaaaat aatccattgc aagcggataa tttttctcgg
     2341 ttcggttcat cttacccatt gccaggtata tatccgacaa tccacctttg tagtgaattc
     2401 ctgaacaata cgtttcagca tctttaaaca atgtaactga ttgctcttta tcgaaggaat
     2461 acattctcct tgcaaggaga atcatggcat cggccttttt ggttcctgag agctctgcgc
     2521 ttacttttcg caatgaatct atttccttac tctgtgaaaa tgcatttatc acagcgcaca
     2581 acatcaggca gacgattgta atcgaatgaa ttaccggttt ttggagcatg gtgtccaaat
     2641 ttaattattt gtgtgagtgt gctaactttt ttctgatgca gatttgatta tttttggatt
     2701 caaatcccat cttttatgcc aaaaggaatc tacaatgtac cggctgcaca caacgaaccg
     2761 gtaaaaagct acgcacccgg aagtgctgaa cgaaaagaat tacaggcgat gcttaaagcc
     2821 ttacgttccg taaaaacgga tatccccatg tacattggcg gaaaggaagt tagaagtggg
     2881 gtaaaatcca ggctggcgcc tccgcatgac catcagcata cactgggttt ttaccataag
     2941 tcagacaaaa agcatattaa gcaagctata gatgctgcac taaaagccaa gaaaaactgg
     3001 gaggatcttt cctgggaaca tcgggctagc atttttctga aggctgccga actgattgca
     3061 ggtccctacc gtgcaaaact gaatgcggcc actatgctcg gtcaatcaaa gaacgcctat
     3121 caggccgaga tcgattcggc ctgcgaaata atcgactttt tgcgcttcaa tgtgcaattc
     3181 atgaccgaaa tctatgccca gcaaccggtg tcttcacccg gaatttggaa ccgtatggaa
     3241 tggcgtccac tcgaaggatt tgtatatgca ctcactccgt tcaattttac ggctattgct
     3301 ggaaacctac cggcaagtgc ggcaatgatg ggaaatgtag tggtatggaa accttccaat
     3361 actcaggtct attctgccaa cgtattgatg gaaattttca taaaagcagg attgccggat
     3421 ggcgttatca atctgattta ccccagtggt ccggacgcag cagaagtgat attcaatcat
     3481 gctgattttg caggaatcca ttttacggga agtacagcgg tttttcagaa tatctggcag
     3541 accattggta acaacatcca caaatacaaa agttatcccc gcattgttgg cgaaaccgga
     3601 ggaaaagact tcattcttgc ccaccccagc gcagatcctg ccattgtagc aactgctatt
     3661 tcaagaggtg catttgagtt tcagggacag aaatgttcag cagcttcaag agcgtatgta
     3721 ccttccaata tctggccgga agtaaaaaaa caggttcttg ctgatctgaa aagcatgaaa
     3781 atgggtggca cagaagattt caccaatttc atcaatgcgg ttatcgatga aaaatcattt
     3841 gataaactta cttcctttat catgcaggcg aaaaaagaca aaaatgcaga agtcattgca
     3901 ggcggaaaat gcgataagaa aaaaggatat ttcattgaac ctacaattat ccaggcaaaa
     3961 gatccacatt atgtaactat gtgcgaagag ttgttcggac ctgtactgac tgtttatgtg
     4021 tacaaacccg aaaaattcaa cgaaactgta aaattggtcg acactacttc catttatgca
     4081 ttaaccggtt ccatcatttc acaggatcgt tatgcaattg aatacgcaac ccgcaacctc
     4141 agaaatgctg ctggtaattt ttacattaac gataaaccaa ccggagctgt agtcggtcaa
     4201 caaccctttg gcggagcaag aggctcaggc accaacgata aggccggatc aatgatcaac
     4261 cttctgcgct gggtttcccc cagaacaatt aaggagactt ttgttccacc tgtggattac
     4321 cgttatccgt tccttcagaa agattaaatc cgaaagtcct gcaaatgcgg gacttttttt
     4381 tgcagtaaag caggtttcta caaacaggaa cgatttccct atgtcgggcg aatcattttt
     4441 tttattaaat aacagaaaat taatgcctat gaaaacgctt ttttccatcg ttttttgttg
     4501 cgcttccgtt gcactattcg cacagcatcc tcaagcagga aaacaaatac acgctcctac
     4561 acaagcaaat acggtcactc cttcaaagga caagcagcct actgtaaata gtcaggttgg
     4621 tacaaaatca caggcttccg gaaataatgg aacagtttat tcagaaaaac aggttcctac
     4681 agaaaattat tcatcgggtc gtacacgtca aacaagcacc ccatcccgct ccggcacagc
     4741 tactcattca gcttcacagc gaaatccgga atacgatgtt gaggagaata ttcccgttga
     4801 cattgatgct ttgcaattgg agctgaggaa ctgtaataac aaaattcaga taagcgtact
     4861 ttagtggttt aacatgacag aaacccaccg caaattgatg ttggcaaatg ccgataaata
     4921 ttgttttgat gaccttagtg tcctgtttat gcttcatctg gcacctgcct gcgcaacaat
     4981 tgcttttttc tgaaaagaat caattatcat ttttaacggc tccgttcttc acggggccgt
     5041 ttttttatgt ggctattgat tacatttgag aaaaaaatct cccatcatga ccaaaattct
     5101 cgcaaacgac ggtatcgaca aatccggaca agtgcttctc gaaaaggcag gttttcaggt
     5161 aatcactgaa aaagtagctc aggaccaact ggctaatgct atcaacgaaa acaattacga
     5221 agtggtcctt gttcgatcag caacaactgt ccgcaaggaa gtgatggatg catgtcccaa
     5281 cctgaaaatg attggtcgcg gtggtgtagg aatggataat attgatgttg cctacggacg
     5341 tgaaaaagga attgatgtat tcaacactcc cggcgcatct tctcaatcgg tagcagagct
     5401 ggtgttttcg catttgttca gtatggctcg ttttctctat gatgccaacc gtaacatgcc
     5461 ggtaaagggt gaaacagaat ttgataaact caaaaagaac tacgctaaag ggatagaatt
     5521 acgaggtaag accatcggaa ttattggttt cggaagaatc ggacaatctg ttgcaacgta
     5581 tgctcttggc tgtggaatga aagtaattgc acacgatcca ttcgtaaaag atgcacatgt
     5641 tgaacttgaa attcacggtc acggcaaagt gaaagtgaac attaaaagtt cttcaatgga
     5701 tgaattactt gccaatgccg atttcatttc attgcatgtc cccaaacaaa aagacggatc
     5761 tgcagtaatt aagtcagctg aaattgaaaa aatgaaagca ggagtttgtc tggtgaacac
     5821 tgcacgtggt ggagtaatta acgaggacga tctgattgcg ggactgaaca gcgggaaaat
     5881 tgcacatgcg gcattggatg tattcgaaaa tgagcctaca ccgcgcaaag accttctttc
     5941 ccaccctaag atttcactta ctcctcacat cggcgcttct actgtagaag cacaggatag
     6001 gattggtgtg gagatcgcag aaaaaatcat ttcgttttac aagaagtaat ttattgttat
     6061 ggcggtaatc cgtcccttta aagcgattcg cccaacacgc gacaaggctc atctggtagc
     6121 atcccggtcg gtgaaccttt acaagcaacg cattctaaat gccaagctcg aagagaatcc
     6181 ctataccttc atgcatgtca ttcttcctga gttcggatca aaaatcacta ctaaaccaaa
     6241 tacaactgaa cggttcaagc tggtgaagaa aaatttcgaa gcctttcgga aaaaggggat
     6301 ttttattcag gataaaaatg attgtcttta tatctaccgt cagctaaaag acggctactc
     6361 ctacaccgga atcattgcag gtgcagcggt ggatgattac ctgaatggtg taatcaaaat
     6421 tcatgagcaa acacttacca aacgagaaga aatttttaaa aactacctcg atgtatgtgg
     6481 attcaatgca gaacctgttt tactctctta cagtgacaac aacactgtag ataaagtttt
     6541 gaaacgttac acagccgaac gatcggaata tgaatttacc acagcagatg gcgtaacaca
     6601 tttcctgtgg attgttgctg agaagaaaga tattgccatt atttgcaaag cctttgagaa
     6661 aattcctgct gtatatattg ctgacggaca tcatcgaagt gcatcatccg ctttgctggc
     6721 aaaagatcgc cgctctaaac tgaaaaaata cagcggtaaa gaaatgttca attactgcat
     6781 ggcctttttt atttctgaaa gtcagttgaa catttacgat tttaaccggg tagtaaaaga
     6841 tctcaatggt ttaagtgcaa aggaatttct tgatcaactg cgtgaaaaat ttgatgtgga
     6901 gtcgaaagga aaaaagtgtt accgtccaaa aaaacttcac aatttcagca tgtaccttga
     6961 aggggaatgg tattcgctta ctgcaaaaaa aggaagtttt aattccaaac atccggtggg
     7021 aaatcttgat gcacaaattc ttaccgacaa cattctctcc cctgttttag gaattcatga
     7081 tctaaagacc gattcccgaa ttgaatttgt tggtggatta caaggcatgg aaggtttgca
     7141 gcgtaaggtt gatgaaggaa aaatgaaagt tgcgtttgga ttgttccctg taaaagtgga
     7201 tcaactcaaa cagattgcag ataccaacaa catcatgcca cccaaaacga cctggattga
     7261 accaaaactg agaagcggat tggttattca gagtcttgat tgatttacga ataaggaaaa
     7321 tgtcaattag cgaaaacctg caccacatcg aatcggaaat tccagcgcat gtaagacttg
     7381 ttgctgtttc gaaaacaaaa ccaaacgaac agattctgga agcttatgca tacggtcagc
     7441 gtgatttcgg ggaaaattat attcaggaat taacggccaa agctgaagca cttcccaaag
     7501 acatacgctg gcatgccatt ggacatattc aaaccaacaa ggtaaaatac attgcacctt
     7561 ttgtacacct cattcacggt gtagacagcc tgaagctatt acaggagatc aacaaacaag
     7621 ctgcaaaaag caatagagtg attgattgtc ttctgcaggt tttcattgca agcgaagaaa
     7681 gtaaattcgg actggatgaa aacgaagtaa aagaaatttt aagtgacgaa attattcgaa
     7741 cactacccaa tattcgaatt gttggtttga tgggaatagc ctccaatact gatgatgaag
     7801 atcaagttcg aaaagaattc cggaatttga aatcactgtt tgattctgta aaacaacttc
     7861 atccaggcat caccatactc tctatgggaa tgagcggaga ttataaaatg gcaattgaag
     7921 aaggaagcac catgattaga gttggaagct taatttt
//
LOCUS       DAOMYG010000092         7951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3016_length_7951_cov_9.813576, whole genome shotgun sequence.
ACCESSION   DAOMYG010000092 DAOMYG010000000
VERSION     DAOMYG010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7951
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3016_length_7951_cov_9.813576"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PK610_04330"
     CDS             complement(<1..648)
                     /locus_tag="PK610_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HRJ35187.1"
                     /translation="MIYLLLSILFSTSLLVIFKFFEKNKINNTTGIVVNYITAASTGF
                     LMSGNIPDPARMFASSWSPWALLIGSVFISLFFIVALSAQRNGMAITSVANKMSLVIP
                     VIAAMFLYNEQLSLLGAGGIVLALCGVIFTAIPSQQSGGGIALASWLFPALIFFGSGI
                     SDTLIKYAETYHLQSVSREYFTAVLFSAAGIWGLFYFILQQIRNKFKVPAKDILAG"
     gene            complement(645..1691)
                     /gene="ribD"
                     /locus_tag="PK610_04335"
     CDS             complement(645..1691)
                     /gene="ribD"
                     /locus_tag="PK610_04335"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRJ35188.1"
                     /translation="MQKHKQFMQRALELAASGSGLTAPNPMVGCVIVCEDKIIGEGFH
                     QRYGGPHAEVNAIASVNDQELLLRSTLYVTLEPCSHFGKTPPCSDLIIEKGIPHVVVA
                     VTDPHAAVNGKGIQRLREAGIEVDTGVLEKEARRLNKRFFSYHEKKRPYIILKWAQTT
                     DGFIDKKRDSAIAMQNRISGEESKLLVHKWRTEEQAILVGKNTVLFDDPQLTARLFSG
                     PQPLRIVFDKHLELPAGKKIFNSDARTLILNAKKSGDEGNLLYHTIDWSNALASLMEL
                     LNSMQIQSLLVEGGSDTLQRFINEGLWDEMRIFHSPLRFAEGTPAPLVQVRESTFSAV
                     GNDQLEWIFNDRQS"
     gene            1836..2708
                     /gene="prmC"
                     /locus_tag="PK610_04340"
     CDS             1836..2708
                     /gene="prmC"
                     /locus_tag="PK610_04340"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRJ35189.1"
                     /translation="MQLPSDNRFSTTVERFHELLDTEWGREEVKAILSILAAEYLGWN
                     RADMVLNKEKALSESELLKFHFALKELLRARPVQYIIGHTPFYGSSFRVDENVLIPRP
                     ETEELVDLICKTYQNTAAKILDIGTGSGCIAVSLAKHLPLAEVSAIDVSPGALQLARA
                     NAESNQVNIHWHELDILQADSNFFPAKQLDVIVSNPPYVLKDEASSMKRNVLEHEPHL
                     ALFVEDSDALLFYRRIVALAEHWLKQGGRLYFEINEQFAQEGISLFSELLWRNTQIHH
                     DLQGKDRMLSAEKI"
     gene            2763..4907
                     /locus_tag="PK610_04345"
     CDS             2763..4907
                     /locus_tag="PK610_04345"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ35190.1"
                     /translation="MLRRIVFVLFVALQSVSTYAANLDSLLLNVERAPSDTAKGRALL
                     ELGRWYIEVNPDTTLILVEQGISLLSASSSRELLCDAYRLKGGVLRFRGEYNEAMYWF
                     KKALAVLEGSKDLRRYGSSYNNVGLVYWDLGNYVLAIENLLISLKATEASGNYESMIS
                     TLINIGLVYDYQDKVELAKHYYARAASIAKKVNDLSSLSLTYNNLGYMYMRSKDFDSS
                     AMYLHASLRLAIDNRYMPNAGYATNNLGALHAEMKQMDSAYFYIDSSLRIREKLGDQF
                     GIGMCYLNFGKWDLEAKKYSSSEKWLLKTKELADITGSKVLIMDVAQALAALYQASGD
                     YRKAYTNMETYYQLKDSLGNEESRRKTVEMRSEFEIDKKQKELDLVRKDAQIDSIAKE
                     SELKRQRLMIGFTLALVALMLFFLVFVYRQFKAKKKANEEITVQKSIIEEKNKEILDS
                     IHYARRIQRALLASDHLLKKHLHEYFVLYQPKDIVSGDFYWASEATVKDAKSAGQKSF
                     TTDETSGHKFIFCVADCTGHGVPGAFMSLLNITKLNEVITERGERIPSVIFDKVREEL
                     IASLNPEGVEEESKDGMDAVLIAFDRSSGNLQLACANNPVWIIRSGELIELKPDKMPV
                     GTHHGTLKPFTPHDFKLHSGDMIYLFTDGYADQFGGPKGKKFKYAQFADLLKEISSWP
                     AEQQKLRLQEVIAQWKGNLEQNDDILIVGIRV"
     gene            4932..>7951
                     /locus_tag="PK610_04350"
     CDS             4932..>7951
                     /locus_tag="PK610_04350"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRJ35191.1"
                     /translation="MIRLLRFIPVLLIVLPFPLRAEKDTSFFLGQPRITKALPSRYET
                     GVRFGVRFLDVEQGLPSSYIMGAAQDSLGRMWFSTDGGGICVYDGDVFQLYGTEEGIP
                     LASGNVFFFDSRGDLWYGNSNKGIIRIHGNSVTHFRHDSLINHNLAFIREDQKGTIFI
                     GSGNQVYRLEKDVLVQQETNTVRAGAGLRDLRFLNKQIYLAAGDSLYIFDSDWNFLAL
                     HAVPVQKIIPIENDHVQLVTRDALIDFNKGKQKVLIADLRTGIDMISDGVYDARSKTT
                     MLASWGRGVILHSEKGVLFFDRRFGMRSDRLYTIFIDNKQRIWYCTDGGGVQVLTAPS
                     NENFSESAGLSVPYIMEIAEVDQELWVGTRGGGMFRRTNNADVPFEKFSEQSGLKSDY
                     VYAIHQTASGEVWIGTHRDGLFIRRNGKFIRHDTKSGFYSNTVWGIESDASGKVYLAT
                     FAGLVIWDGNSFNMYTTDHGLPGNRIYSIAIDKKGEVWLGTSAGLACYHSDGKIDAYT
                     RSDGLPDDIVWDIEPTADGLWLATHGGICRFENGVFTVWNVSHGLSTNMVWALKWSDD
                     QLWAGTEKGVQCFSLVQSQLQLSRTLGFADGFTGNDVTPASIYLQKNGKILFGTSRQL
                     VEYDPAFDKVGKEPALQPLLLGLELFYRTYDFAASGDSLPVFGSLPHGMVFAHDQNTI
                     TLSYLSVDVLSSGALSYRIELEQDNEKISAQISNQQKVTFTNLAPGKYKISISASSDG
                     VVWSEPAQWSFSILPPFWQTWWFRILFLLVAGGMVWLFILFRTRALRKSQKRLEGLVK
                     ERTAEITLKNDMLEEKNKEILDSMTYARRIQNALFASDELLSRSLNEFFLFYRPREAV
                     SGDFYWASQVVDNNEERFLICVADCTGHGVPGAFMSLISIAFLNEAIRERKITSPGSV
                     LNHVREDIIRALDQEGKNESRDGMDAVLLSFSKDLRNVRFALANNPLWIIRRAELIEI
                     KPDKMPVGPHLGVMKDFTQHEMQLESGDQLFLFT"
BASE COUNT         2101 a         1711 c         1958 g         2181 t
ORIGIN      
        1 accagccaga atgtcttttg cagggacttt aaacttgtta cggatctgct gaagaatgaa
       61 ataaaacagt ccccaaattc ctgctgcaga aaatagaacc gctgtaaagt attcgcggct
      121 tacactttgc aaatggtagg tctcagcgta tttgatgagc gtatcggaaa tgcccgatcc
      181 gaaaaagatc aatgcgggaa acaaccagct tgccagcgca attcctcctc cggattgttg
      241 ggatggtatg gctgtgaaaa taactccaca caaggccagt acaattcctc ctgctccgag
      301 taaactcaat tgttcattgt atagaaacat cgcagcaatg acaggaatca ccagcgacat
      361 tttatttgcg acagaggtaa ttgccattcc gttgcgttgt gcgctgaggg cgacaataaa
      421 aaacagactg ataaaaacag atccgatcag caacgcccag ggcgaccagc tgcttgcaaa
      481 catgcgggcc ggatcaggaa tatttccgct catgagaaaa ccggtgcttg cagcagtgat
      541 gtagttgacc acaattccgg ttgtgttatt gattttgttc ttctcaaaaa atttgaagat
      601 caccaatagc gatgtcgaaa aaagaatact tagcagcagg tagatcatga ctgcctatca
      661 ttaaaaatcc attccagctg atcattgcca acagcgctga atgtgctttc acgaacctga
      721 acaagtggag caggagttcc ttcagcaaaa cggaggggcg aatgaaaaat acgcatctcg
      781 tcccacaatc cttcattgat gaatcgctgc aaggtatcgc ttccgccttc caccaggagc
      841 gattggatct gcattgaatt taatagttcc atcagcgagg caagagcatt gctccagtcg
      901 atagtgtggt acaatagatt tccttcgtcg ccggattttt ttgcattcag aatgagtgtg
      961 cgtgcatcgg aattgaatat tttttttccg gcaggaagtt caaggtgttt gtcgaataca
     1021 atacgcaatg gttggggacc tgaaaacagt cgtgctgtca attgcggatc gtcgaagagg
     1081 accgtgtttt tcccaacgag aatcgcttgt tcttcggttc tccatttgtg caccagcaat
     1141 ttgctttctt ccccactgat gcggttttgc atcgcgattg ctgagtctct ttttttatcg
     1201 ataaaaccat cggtggtttg tgcccacttg aggatgatgt acggacgctt tttctcgtga
     1261 tagctgaaga aacgtttgtt cagccgacgc gcttcttttt cgagaacacc ggtgtcgact
     1321 tcgatccctg cttctcgcaa tcgttgaatg ccttttccgt ttaccgctgc atgaggatct
     1381 gttaccgcga caactacatg tggaattccc ttttcgatga tgagatcgga acagggaggg
     1441 gttttgccga aatgcgaaca aggttcgagc gtgacgtaga gtgtcgatcg aagcaggagc
     1501 tcctggtcgt ttaccgaagc gatggcattc acttctgcat gcggaccacc ataacgctga
     1561 tgaaaacctt caccaatgat tttgtcttca caaacaatca cgcagcccac catcggattg
     1621 ggagcagtaa gtcccgatcc cgaagcagca agctccagtg cgcgttgcat aaattgcttg
     1681 tgtttttgca tggcacgaat ttacatgatt tttagcagcc cgcttcatgt cagtgtaaag
     1741 gaagaaaaag ccattttccg aaggccaatt cggtatctca attcagaatc tctcttgata
     1801 atagcaccaa aatcaggcaa tttctatctt tgagcatgca attgccatcc gacaaccgat
     1861 tcagcacaac cgtggagcgt ttccacgaac tgctggatac ggaatggggc agggaagagg
     1921 tgaaagccat cctttccatt cttgctgcgg aatacctggg atggaacaga gccgatatgg
     1981 tgctcaataa agaaaaggca ttatcggaat cggaattgct gaagtttcat tttgcactga
     2041 aagaattgct gcgcgcgcga ccggtgcagt acattatcgg acatacaccc ttttacggat
     2101 cttcattccg tgtggatgaa aatgtgctga taccccgtcc cgaaaccgaa gaactggtgg
     2161 atctgatctg caaaacctat cagaatactg cagcaaaaat tcttgatatc ggaacaggca
     2221 gcggatgtat tgcggtttcg cttgcaaaac atctaccgct tgcagaagtg tccgctattg
     2281 atgtctctcc cggagccttg caactggcac gtgcgaatgc ggaatccaat caggtaaaca
     2341 ttcattggca cgaactggat attttgcagg ccgattccaa cttttttcct gcaaaacaat
     2401 tggatgtgat cgtgagcaat ccgccttatg tgttgaagga tgaagcatcc tccatgaaac
     2461 ggaatgtgct ggagcacgaa ccacatctgg ctttgtttgt tgaggatagc gacgctttgt
     2521 tgttttaccg gcgtattgtt gccttggcgg agcattggtt aaagcagggg ggacgattgt
     2581 atttcgagat caacgaacag tttgcgcagg agggaatctc acttttttcg gaattgctgt
     2641 ggagaaacac acaaattcac cacgatctgc aggggaaaga ccgtatgctg tcggctgaaa
     2701 aaatctgatc ggcatgtacg aatagtgatt atattgtaat cgcttttcac caacaattgc
     2761 cgatgttacg cagaatcgtt tttgttttgt ttgttgcctt gcagagtgtt tccacttatg
     2821 cagctaatct cgattcttta ttgcttaatg tagagcgtgc tccttcggat actgcaaaag
     2881 gaagagcatt actcgaattg ggcaggtggt acatcgaagt aaatcctgat accacgctta
     2941 ttctagtgga gcagggaatt tcattgttgt ctgcatcttc ttcccgcgaa ttgttgtgcg
     3001 atgcttatcg gttaaaaggc ggtgttttac gttttcgcgg tgaatacaat gaagccatgt
     3061 actggttcaa aaaagcactt gccgtactcg aaggctcgaa ggatcttcgt cgttatggaa
     3121 gttcctacaa caatgtcgga ctggtgtatt gggatcttgg caattatgta cttgctattg
     3181 agaatttact catctcactt aaggcaaccg aagcttccgg taattacgaa tcgatgattt
     3241 ctacactgat caatatcgga ttggtgtacg attatcagga taaagtggaa ctggctaaac
     3301 actattacgc gagggcagcc agcattgcca aaaaagtgaa tgatctttct tcgctttctc
     3361 ttacctacaa caatcttggg tacatgtaca tgcgcagtaa agattttgac agctctgcca
     3421 tgtatttgca tgcttcgctt cgattggcta ttgataatcg ctacatgccc aatgccggat
     3481 atgccaccaa caatctcggt gcactgcatg cggagatgaa acaaatggac agcgcctatt
     3541 tttacattga ttccagtttg cgtattcgag aaaaattggg tgatcagttt ggtatcggaa
     3601 tgtgttacct gaatttcggg aagtgggatc tggaagccaa aaaatattcc agctcagaaa
     3661 aatggctgct caaaaccaaa gagctcgcag acatcaccgg atcaaaagtg ctgatcatgg
     3721 atgttgcaca agcgcttgct gctttgtacc aggccagtgg cgattaccgc aaggcgtaca
     3781 ccaacatgga aacgtattat cagctgaagg attcattggg caatgaggag agtcggagaa
     3841 aaacagtgga gatgcgttct gaatttgaga tcgataaaaa acaaaaggaa ttggatcttg
     3901 tccgcaaaga tgctcagatc gacagtatcg ccaaggagag tgaactgaaa cgtcagcgac
     3961 tgatgatcgg ttttacactt gcgctggttg cattgatgtt gttctttttg gtgtttgtgt
     4021 accggcagtt caaagcgaag aaaaaagcaa acgaagaaat tacggtgcaa aaatccatca
     4081 tcgaagaaaa gaacaaggag attctcgaca gtattcacta tgcacgaaga attcagcggg
     4141 cattgctggc ttccgatcat ttgctcaaga aacaccttca cgagtatttc gttttgtatc
     4201 agccaaaaga tattgtaagc ggcgatttct attgggcatc ggaggcaaca gtgaaggacg
     4261 caaaaagcgc aggacaaaaa tcattcacta cggatgaaac ctccggacat aaatttattt
     4321 tttgtgttgc cgattgtacc ggacatggtg ttcccggtgc gtttatgtca ttgctgaata
     4381 tcaccaaact caacgaagtg atcaccgaac gaggtgaacg tattccttca gtaattttcg
     4441 ataaggtgcg cgaagaactc atcgcatcgc tcaatcccga aggtgtggaa gaagaatcga
     4501 aagacggaat ggatgctgtt ctgattgcct tcgatcgttc ttcagggaat ctgcaactgg
     4561 cttgtgccaa taatccggtg tggatcattc gctcggggga gctgatcgaa ctcaagcccg
     4621 ataaaatgcc ggtgggtact caccacggta ctttaaaacc atttacccct cacgatttca
     4681 agctgcacag tggcgatatg atctacctct ttaccgatgg ctatgccgat cagtttggcg
     4741 gacccaaagg caagaagttc aagtacgcgc agtttgccga cttgctgaaa gaaatttctt
     4801 cctggcctgc cgaacagcaa aaacttcgtt tgcaggaggt aatcgcccaa tggaaaggta
     4861 atttggaaca aaacgacgat attctcatcg tgggtatccg tgtctaatag ccaatcccgt
     4921 atatttactt cgtgatccga ctgctgcgct ttattccggt actgctgatt gtcttgcctt
     4981 tcccactgcg ggcagaaaag gatacttcgt tttttctggg tcaaccacgc attacgaaag
     5041 ccttgccttc gcgttacgaa acaggtgtgc gctttggtgt acgtttcctt gatgtggagc
     5101 agggattgcc ttcctcgtac atcatgggtg ctgcgcagga ttcattggga agaatgtggt
     5161 tctctaccga tggcggtgga atttgtgtgt acgatggaga tgtctttcag ttgtacggta
     5221 ccgaagaggg aattccgctg gcttccggaa atgttttctt tttcgattcg cggggtgatc
     5281 tgtggtacgg taacagcaac aaaggaatca ttcgcattca tggcaattcc gttacgcatt
     5341 ttcgtcacga cagcctgatc aatcataacc tggcttttat ccgcgaagat cagaagggga
     5401 cgatcttcat cggttcggga aatcaggtgt atcgtctcga aaaggatgtg ctcgttcagc
     5461 aggaaaccaa tacggtgcgt gcaggtgccg gattgcgtga tctgcgtttt ttgaataaac
     5521 agatttacct ggctgcaggt gattcgttgt atattttcga tagtgattgg aattttctcg
     5581 ccttgcatgc tgttccggtg caaaagatca ttcccattga aaacgatcat gtgcaattgg
     5641 tcacccgcga tgcgctgatc gatttcaata agggaaaaca aaaagtactc attgcagatc
     5701 tgcgtacggg tatcgatatg atttcggacg gtgtgtatga tgcgcgcagc aaaacaacca
     5761 tgctggctag ttggggcaga ggagtgattc ttcattcgga aaagggtgtg ctcttttttg
     5821 atcgtcgctt cgggatgcgg agtgataggc tgtacacaat ttttattgat aacaaacaac
     5881 gcatttggta ttgcaccgat ggcggtggtg tacaggtgct cactgcgcct tccaacgaaa
     5941 atttttcgga gtcggcagga ctcagtgttc cttacatcat ggaaattgca gaagtggatc
     6001 aggaattgtg ggtgggtacg cgtggtggtg gtatgtttcg caggacaaat aatgcggatg
     6061 ttcccttcga aaagttcagc gaacaaagcg gattgaaaag tgattatgtg tacgccatcc
     6121 atcaaacggc ttcgggagaa gtgtggatcg gtacgcaccg cgatggtttg ttcattcgcc
     6181 gtaacggaaa atttattcgc cacgatacga aatcaggttt ttattccaat accgtttggg
     6241 gaatagaaag cgatgcatcc ggaaaagttt acctcgcaac gtttgcagga ttggtgatat
     6301 gggacggtaa ttctttcaac atgtacacta ccgatcacgg attgcccggt aatcgtattt
     6361 acagcatcgc cattgataaa aaaggtgaag tttggctggg aacctctgca ggattggcct
     6421 gttatcacag cgacggaaaa attgatgctt atacacgcag cgatggtttg ccggatgata
     6481 ttgtgtggga tattgaaccc acagctgacg gattatggct ggcaacgcac ggtggtatct
     6541 gtcgtttcga gaatggtgtg ttcacagttt ggaatgtgag tcatggttta tcgaccaaca
     6601 tggtttgggc gctcaaatgg agcgacgatc aattgtgggc gggtaccgaa aaaggggtgc
     6661 agtgtttttc gctggtgcaa tcgcaacttc aactcagcag gacactcggt tttgcagatg
     6721 gatttaccgg gaatgatgta acacctgctt ccatttatct gcagaagaat ggaaaaattc
     6781 tttttggtac ttcacgtcaa ttggtggaat acgatccggc tttcgataag gtaggaaaag
     6841 aaccggcatt gcaaccgctc ttgctcggac tcgaattgtt ttaccgcact tacgattttg
     6901 ctgcatccgg agattccctt cctgttttcg gatcacttcc ccacgggatg gtgtttgcgc
     6961 atgatcagaa taccatcacg ctttcttatt tgtcggttga tgtgctttca agcggcgcac
     7021 tatcgtaccg tatcgaattg gagcaggaca atgaaaagat cagtgcacag atcagcaatc
     7081 agcagaaagt aacgtttacc aatctcgctc ccggaaaata caagatcagt atatccgctt
     7141 cttccgatgg agtagtgtgg tcggaacctg cgcagtggtc gttttctatt ctgcctcctt
     7201 tttggcagac ctggtggttc cggattttat ttcttcttgt ggctggagga atggtgtggt
     7261 tgttcattct gttccgcacc cgcgcattgc ggaaaagtca gaaacgtctc gaaggattgg
     7321 taaaagaacg cacagcagag atcacactga aaaacgatat gctggaagag aaaaacaagg
     7381 aaattcttga ctccatgacc tacgctcgga gaattcagaa tgcattgttt gcctccgacg
     7441 aattgctctc acgatcgctc aatgagtttt ttctcttcta tcgtccccgc gaagccgtaa
     7501 gtggcgattt ttactgggcc tcgcaggtag tcgataataa cgaagaacga tttttgattt
     7561 gtgtagcaga ttgtacagga cacggtgttc ccggtgcttt catgagtttg atcagtatcg
     7621 ccttcctcaa tgaagccatc cgcgaacgaa aaataacatc acccggcagc gtactcaatc
     7681 atgtgcgcga agatatcatc cgtgcgctcg atcaggaagg aaaaaacgaa tcgcgcgacg
     7741 gaatggatgc tgtattgtta tccttcagca aagatttacg caatgttcgt tttgcacttg
     7801 ccaataatcc gctttggatc attcgtcgtg ctgaactcat cgaaataaaa ccagataaaa
     7861 tgcccgttgg tccgcacttg ggtgtgatga aagatttcac gcaacacgaa atgcaactcg
     7921 aatccggcga tcagcttttt ctcttcaccg a
//
LOCUS       DAOMYG010000093         7912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3044_length_7912_cov_7.068219, whole genome shotgun sequence.
ACCESSION   DAOMYG010000093 DAOMYG010000000
VERSION     DAOMYG010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7912
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3044_length_7912_cov_7.068219"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..1062)
                     /locus_tag="PK610_04355"
     CDS             complement(163..1062)
                     /locus_tag="PK610_04355"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRJ35192.1"
                     /translation="MQRTFTEDLRHQFKTGSIYTKLIILNTAVFLVMIFISIFLHLNN
                     YPRPIAIAAILDWFAPSGNMQHNLTHPWSIFLYMFLHIDVFHFIMQMFLLYFTGRIFM
                     EYLDEKKLLNTYIFGGLSGLLFYITAMNVFPVFNGFENSPLLGASAATLALLVAVSFH
                     LPQTEIMLFGAFRVKLIYIALLFIAIDLIAIPNDNRGARIAHLGGTFYGIMAAGFFRK
                     PKFVLNTFDLTTLFSPEQWFKKKSRMKVEYSKRPISDIEYNENKVSKQKRIDSLLEKI
                     NRSGYESLTKEEKDFLFKASKDV"
     gene            complement(1066..1752)
                     /locus_tag="PK610_04360"
     CDS             complement(1066..1752)
                     /locus_tag="PK610_04360"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRJ35193.1"
                     /translation="MIEHYFRTMPPVIKNFLIINVLVFLATSMYVGPDFNLKSFLAVY
                     FVKSDFFQPYQIITHMFTHVNFMHLFFNMFAMVMFGTILERQLGTKRFFILYFSCGLG
                     ALALYQSVAFIEYSVYLDQINSGTNSIAAPFALLEILYTPAMGASGAIFGMLMLFMLL
                     YPETEFILFPIPFRIKAKYYLPVLIYIEFYFGLQQLKGNESNVAHFAHLGGALIGLVF
                     YLVWRKKRIV"
     gene            complement(1752..3566)
                     /gene="mutL"
                     /locus_tag="PK610_04365"
     CDS             complement(1752..3566)
                     /gene="mutL"
                     /locus_tag="PK610_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRJ35194.1"
                     /translation="MSNIIRLLPDSVANQIAAGEVVQRPASAVKELMENAVDAGASKI
                     RLVVKDAGRTLLQVIDNGCGMTADDAQLSFVRHATSKIGNAEDLFRIQTKGFRGEALA
                     SIAAVAQVEMKTKRREDELGTRIVIEGSEIQEVHADPCQDGTQFLVKNLFFNIPARRN
                     FLKSDNVEFKHILEEFERVALPHPSIQFELNHNGNEVFNLPSQNLRQRIVALFGKTYN
                     ERLVPIEENTEIVAIKGFLLKPEFTRKTRGEQYFFVNDRFIRSPYLHHALQKAYEGLI
                     PKENFPGYFVFLSVPTNSIDVNIHPTKTEIKFEDERSIYAILHSAARRAIGFHNISPS
                     IDFNTETAIDLPLPDKDRIPQAPVIRVDPNYNPFDSEKKNTAYPTEYKHQKKQAAQNW
                     ESIYETLKEEPRKQEQLFEEGSDVNQKRVTVQLQGKYILCPVKSGFWLIDQHRAHSRI
                     LFERFIVSLAQHTGMSQQMLFPTMVELTPGDFALVRDLDEDLRHLGFELSDMGGNSIA
                     VNGVPAEAGDIHPGTLIEKMLEDLKHSGNAAGKEKSERIARSLAESLAIKTGKTLMTE
                     EMNRLVDELFACESPYVSPSGKPVIVTFTAEELEKRFA"
     gene            complement(3573..3890)
                     /locus_tag="PK610_04370"
     CDS             complement(3573..3890)
                     /locus_tag="PK610_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35195.1"
                     /translation="MISLRSNYFKTKKPRQFSYLPRYYNERKENLELRKKAFSNEAEE
                     ISAQDMETRIRQGFSRGGIQRNHNSRNHLRQNNTNRMVILVGLVCTALYVIYKMDLWE
                     FFF"
     gene            4066..7047
                     /locus_tag="PK610_04375"
     CDS             4066..7047
                     /locus_tag="PK610_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRJ35196.1"
                     /translation="MNKSISILLAGAFLLLLLSSFVFPVDSKGPSTNPLWKQKPRFLD
                     VDSRWADSLMLVMSPEERIAQLFMVAAYSNRDQVHEAEIMHLVTEHKVGGLIFFQGGP
                     MRQAHLTNRYQSAARVPLMIGIDGEWGLAMRLDSTMKFPKQMTLGAINNDTLVYLMGL
                     EIGRQCRRLGIHVNFAPVVDVNSNPDNPVINFRSFGEDRVNVARKSFAYMLGLQDKFV
                     LANAKHFPGHGDASADSHHTLPVINRTKKQLDSLELFPFRYLIRTGLASIMVAHLYVP
                     ALEKAENTATTLSKKVVSGLLKKDMAFEGLVFTDALNMQGVSAFYKPGEVELKALLAG
                     NDVLLFAEDVPNAINLIKGAIARGEISQADIDQRCRKLLLAKKWVGLDRERLVKTEGL
                     MQDLHTPYGKLIQRKLYQESLTLLENKNEILPITDLEKKTIAIVTLGDTMNNPFMQSV
                     SRYATVKKFALGLEPKGSEIGRLIENTRDVNLFIIGLHPFTNAPKDNYGLTEEAIGLV
                     NQLNQSREVVLAYFGNAYGLRHFKGASLLDGLIMCYENSSVGQDLAAQAIFGGIGVEG
                     TLPVNVSEYFHLNEGMCFSGGMRFEYTSPEAVGIRSSNLDKIDSLALLGIREKAYPGC
                     QILIAKKGKVFYNKSFGFHTYENNRPVREDDIYDLASVTKITSTVAVLMHLTDQEKFK
                     LDSKLGDYIPEILDSSVYSKINIRKMLAHEAGLVPWIPFYVKTLKNGLPDPRIYRTTP
                     SDSFPYRVAENLYILKTYKDSIIKEIKSTPLRNQRNYLYSDVGYYLLQEIIQKITGKP
                     LDVLTDSLFFKPLGATTLCYNPRKQFPLDRIPPEEDDKVFRRQLIQGDVHDQGAAMLG
                     GVGGHAGLFASANDLAKMMQMLMNYGEYGGRRYLSAQVVKEYTGCQFCPTNRRGAGFD
                     RPTMSDGLGPTCNCVSYESFGHTGFTGITAWADPGEDIIYLFLSNRSYPIADNNKILK
                     MGLRTDIQQVIYTAIRKGNPF"
     gene            complement(7084..>7912)
                     /locus_tag="PK610_04380"
     CDS             complement(7084..>7912)
                     /locus_tag="PK610_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRJ35197.1"
                     /translation="PYSYTIDRGYMFEKYMNQNLDSVFNKRLYEYIVPEKTSKIPTMM
                     FCPTIINDGRRLIISAQPVSHLVNCRPDVEIDELPGQEYVEFTKLFQDQNAWNLKFSS
                     ALRMSASFPYVMPMVTLPSEPAIEVMDAGYRDNYGIRVALRYIYVYRNWISSNTDGVV
                     ILQIRDRQKEVEENRVKNSVFKRLVKPLGNLYDNIFYTQDFDNDQLLQYAGSWLNSKL
                     DIVEFHMIQNDKQRTSLSWHLTKLEKAHVIQSLDSIPANKIAMEKLRNLLMPAEQQN"
BASE COUNT         2299 a         1596 c         1716 g         2301 t
ORIGIN      
        1 aaaaaaaccg gataggccaa tccgaaaaat gctaccggcc ataaccatga aggatcggta
       61 tagcaagcta aataggagat gagtaatgcc agtccattgc caatggtaaa ccacaatacc
      121 aaacgccaaa caaagttaag cggacggtcc tgtaaaaacc gtttaaacat ctttactggc
      181 tttgaaaagg aagtcttttt cttccttggt cagactttcg taaccggagc ggttaatttt
      241 ctccagcagg gaatcgattc gcttttgttt ggagactttg ttttcattgt attcaatatc
      301 gctgatcgga cgtttgctgt actcaacttt cattcgtgat ttctttttaa accactgttc
      361 gggagagaac agggtagtga gatcgaaagt gttcagtacg aatttaggtt ttctaaaaaa
      421 tcctgccgcc ataatcccgt aaaatgttcc tcccagatga gcgatacgtg ctccacggtt
      481 atcattgggg atagcaataa ggtcgatggc aataaacaat aaggcaatat aaatcagttt
      541 tacccggaac gcgccgaaga gcataatttc tgtttgagga aggtgaaatg aaaccgcaac
      601 caataatgcg agtgttgcag ctgatgcacc aagtagcgga gagttttcga atccgttaaa
      661 tacagggaaa acattcattg cggtgatgta aaacagcagc ccgcttagtc cgccaaaaat
      721 gtaggtgttc aacagtttct tttcatccag gtattccata aaaattctcc cggtaaaata
      781 caggaggaac atttgcataa taaaatgaaa cacatcaata tgcaggaaca tataaaggaa
      841 aatactccat gggtgcgtca ggttgtgctg catgtttccg cttggtgcaa accagtcgag
      901 tattgctgca attgcaattg gacgcgggta attgttcagg tgcagaaaga tggatataaa
      961 aatcatcacc agaaataccg cagtattcag gatgatcagc ttcgtataga tggatcctgt
     1021 tttgaattgg tgacgcaaat cttctgtaaa tgttcgttgc atgatctaca caattcgttt
     1081 ttttcgccag acaagataaa ataccaatcc aattaatgca ccacccaagt gggcaaagtg
     1141 ggcaacgttg gactcatttc cttttaattg ctgtaatcca aaatagaatt cgatataaat
     1201 caataccggc aaatagtatt ttgctttaat ccggaatgga ataggaaata gaataaattc
     1261 agtctcggga tacaaaagca taaacaacat caacattcca aatatggcac cggatgcgcc
     1321 catagccgga gtatataaaa tttctaaaag cgcgaatggt gctgcaatag aattagtgcc
     1381 ggaattaatc tgatctaaat aaacggaata ttcaataaag gcaacagact gataaagtgc
     1441 aagcgctccc aaaccacagg aaaaatagag aatgaaaaag cgtttggtcc ctaattgtcg
     1501 ctccagaata gtcccaaaca tcaccatcgc aaacatgtta aaaaacaaat gcataaaatt
     1561 tacatgcgta aacatatgcg tgatgatctg gtaaggctga aaaaaatcgg attttacgaa
     1621 gtacacagcc agaaaggact tgaggttgaa gtccggtcct acgtacatgc tggtagcaag
     1681 aaataccaat acgttaatga tcaaaaagtt tttgatcacc ggaggcattg tacgaaaata
     1741 atgttcaatc atcaggcaaa tcgtttttca agctcttcgg cagtaaaggt aacgataacc
     1801 ggtttacccg agggacttac gtaaggactc tcacaggcaa acaattcgtc caccaatctg
     1861 ttcatttctt ccgtcattaa tgtttttccg gtttttatgg ccaacgactc tgcaaggctg
     1921 cgggcaatgc gttctgattt ttcttttcct gcagcattac ctgagtgctt cagatcttcg
     1981 agcattttct caattaatgt tccggggtga atatcaccgg cctctgcagg tacaccgtta
     2041 acagcaatgg aatttcctcc catatcgctt aactcaaatc caaggtgacg cagatcttcg
     2101 tccagatccc ttaccaaagc aaaatctccc ggagtgagtt cgaccatagt aggaaaaagc
     2161 atttgctgac tcattccggt atgttgtgca agcgaaacaa taaaacgctc aaataaaatt
     2221 ctcgaatgtg ccctgtgctg atcgataagc cagaaaccac tttttacggg acaaagaata
     2281 tattttccct gcaattgaac agtaaccctt ttttggttga catccgatcc ttcctcaaac
     2341 agttgttctt gcttccgggg ttcttctttc agtgtttcgt agatcgattc ccaattttga
     2401 gctgcttgtt ttttttggtg cttgtattcc gtagggtagg cagtgttttt cttttcgctg
     2461 tcgaaaggat tgtaattggg atcaacccga atcaccggtg cctggggaat tctgtcttta
     2521 tcgggaagag gaagatcaat cgcagtttcg gtattgaaat cgatgcttgg ggagatatta
     2581 tggaagccta ttgctcttcg tgcagctgag tgtaaaatgg cataaatgga gcgttcatct
     2641 tcgaacttga tctccgtctt ggtaggatga atatttacat caatgctgtt ggtgggtacc
     2701 gacagaaaga caaaatagcc ggggaaattt tctttgggga tcaatccctc atacgctttt
     2761 tgaagggcat gatgcaaata gggcgaacga atgaaacggt cgttaacaaa aaagtactgt
     2821 tctccccttg ttttacgggt aaattccggt ttcagcaaaa agccctttat agcaacaatt
     2881 tctgtgtttt cttcgatggg aaccaaacgt tcattgtagg tttttccaaa tagcgcaaca
     2941 attcgttgtc tcagattttg agacggaagg ttgaagactt cattcccatt gtggttgagt
     3001 tcaaattgta tagaaggatg tggtaatgct acacgttcaa attcttccag aatatgtttg
     3061 aattcaacat tatctgattt caaaaaattt cttcgcgcag gtatattaaa aaacaggttt
     3121 tttacaagga attgtgttcc gtcctgacac gggtctgcat gcacttcctg aatttctgaa
     3181 ccttcaataa caatgcgggt ccctaattca tcttcgcgcc gtttggtttt catttctacc
     3241 tgagcaaccg cagcgatcga tgccaatgct tcgccacgaa aacctttggt ctgtattcga
     3301 aaaagatcct ctgcattgcc aattttactg gtagcatgac gcacaaaact taattgtgca
     3361 tcgtcggcag tcataccaca gccattatca ataacctgca agagtgttct gcccgcatct
     3421 tttaccacca gcctgatttt gcttgcaccg gcatccactg cgttttccat caattccttc
     3481 acggcagaag caggacgttg aacgacttct ccggctgcga tctgattggc taccgaatcg
     3541 ggtaataagc ggatgatgtt tgacattggc ggttaaaaga aaaactccca aagatccatc
     3601 ttgtagatca catagagtgc agtgcaaacc agtccgacca aaataaccat gcggttggtg
     3661 ttgttttgcc tgaggtggtt ccttgaattg tggttacgct gaattccacc tcttgaaaat
     3721 ccctgacgga tacgggtttc catatcctga gccgaaattt cttccgcttc atttgaaaaa
     3781 gcttttttgc gaagttccag attctccttg cgctcattgt aataacgtgg cagataggaa
     3841 aactgacgtg gcttcttggt cttgaaataa ttggatcgta gcgatatcat agcagtacag
     3901 ttacaaaatt aacgaaaatt cctcatcaga aaaacggaaa atggcaggga tattgtttga
     3961 aataccacca gagcttaacc aagatgtgtt gataattcag taccggcctc gctttctgag
     4021 ggaagggtct gactattttc gaatccgtgt atgtgtgtaa atcgtgtgaa taagtcaatt
     4081 tctattttgt tggcaggtgc ttttttactg ctgctgcttt cttcttttgt gtttccagtt
     4141 gacagcaagg gcccctcaac caatcctctt tggaaacaaa agccccgctt tctggatgtg
     4201 gattcccgtt gggcagattc gctcatgttg gtgatgtcgc ccgaggaacg cattgcgcag
     4261 ttgtttatgg ttgctgccta ttccaacagg gatcaggtgc atgaagcaga aatcatgcat
     4321 ttggtgacag aacataaagt cggtggactc atcttctttc agggtggacc aatgcgacag
     4381 gctcatctca ccaaccgtta tcaatctgca gcacgggtac cgctaatgat cggtattgac
     4441 ggcgaatggg ggttggccat gcgactggat agcaccatga agtttcccaa gcaaatgaca
     4501 ttgggtgcaa tcaataacga tacactcgta tatctgatgg gattggaaat cggcagacaa
     4561 tgccggcgac ttggaataca tgtcaatttt gcacccgttg tagatgtgaa ttccaatccg
     4621 gataatccgg tcattaattt cagatctttt ggtgaagaca gagtaaatgt tgcccgaaaa
     4681 tcctttgcct acatgctggg attgcaggat aaatttgttc tggccaacgc aaaacatttt
     4741 cccggacacg gagatgcatc tgccgactcc caccataccc tgcctgtaat aaaccgaact
     4801 aaaaagcaac tcgattcact ggagttgttt cctttcaggt atttgattcg aacaggtctt
     4861 gcttccatta tggttgcaca tctctatgtt cctgcactcg agaaagcaga aaatacagca
     4921 acaaccctga gtaaaaaagt cgttagcgga ttgctcaaaa aagacatggc ttttgaggga
     4981 ttggtattta cagatgcatt gaatatgcaa ggagtgagtg cattctacaa gcccggtgaa
     5041 gttgaattaa aagctttgct tgccgggaat gatgtcttgc tttttgcaga agatgttccc
     5101 aatgccatta atctaatcaa aggcgcaata gcaagaggag agatctcgca ggcagacatt
     5161 gaccaacgtt gccggaaact gttgcttgca aaaaagtggg ttggactcga tcgtgaacgt
     5221 ttggtaaaaa cagaaggttt gatgcaggat ctgcatacac cctatggaaa attgattcaa
     5281 cgcaaattat accaggagtc gcttacttta cttgagaata aaaatgaaat tcttccgata
     5341 acagaccttg agaaaaaaac gattgcaatt gtaacgcttg gtgataccat gaacaatcca
     5401 ttcatgcaat cggtatcgcg ctatgccacc gtaaaaaaat ttgcattagg attagaaccc
     5461 aaaggaagcg aaatcggccg gttgattgaa aatacaaggg atgtaaattt gtttattatc
     5521 ggactgcatc cttttaccaa tgctccgaaa gataattatg gattaactga agaggccata
     5581 ggtctcgtta accaattaaa tcagagcagg gaggttgtac ttgcatattt tgggaatgca
     5641 tatggactcc ggcattttaa gggagccagt ttattggacg gattaattat gtgctatgaa
     5701 aattcttcgg ttggtcagga tctggctgcg caggcaattt tcggtggaat aggagtagag
     5761 ggaacgcttc ctgtaaatgt ttcggaatac tttcacctca atgaagggat gtgtttttca
     5821 ggtggaatgc gatttgaata tacaagtccc gaagcagttg ggatccgttc ctcaaacctg
     5881 gataaaatag attccctggc cttacttgga attcgtgaga aggcatatcc cggttgccaa
     5941 attcttattg ctaagaaggg taaagttttt tacaataaga gttttggttt tcatacctac
     6001 gaaaataatc gtccggttag agaagatgat atttatgatc ttgcctcggt taccaaaatc
     6061 acatcaacag ttgcggtttt aatgcatctt actgatcagg agaagttcaa gttggattct
     6121 aaactgggag attacattcc tgaaattctt gattcttctg tttacagcaa aatcaatatc
     6181 cgtaaaatgc ttgcgcatga agcaggattg gttccctgga ttccgttcta tgttaaaaca
     6241 ctaaagaacg gacttccgga tcctagaata tatagaacaa ctccttctga cagttttcct
     6301 tatcgcgttg cagagaatct ttatattcta aaaacataca aggattctat cattaaagag
     6361 atcaaatcga cgccactccg aaatcagcgt aattatttgt acagtgatgt cggctattat
     6421 ttgctacagg agattattca aaaaataaca ggtaaaccat tggatgtgct taccgatagt
     6481 ttatttttta aaccgctggg tgcaacaaca ctttgttaca atccccgaaa acaatttccg
     6541 ctcgatagaa taccgcctga ggaagatgac aaggttttcc gtcggcaact gattcaagga
     6601 gatgtgcatg atcagggagc tgcaatgttg ggcggagttg gtggtcatgc aggattgttt
     6661 gccagcgcta atgatttggc caaaatgatg cagatgttaa tgaattatgg agaatacggc
     6721 gggcgccgat atctttctgc tcaggtggtg aaagagtata ccggttgcca gttttgcccg
     6781 acaaaccgac gcggagcagg attcgaccgg cctaccatgt cagacggttt aggaccaact
     6841 tgtaactgcg tgtcctacga aagctttggt cataccggct ttaccggaat tactgcctgg
     6901 gccgatcccg gtgaggatat catttattta tttctttcta accgaagcta cccgattgcc
     6961 gataacaata agattttgaa aatgggatta agaacagata ttcagcaggt catttatact
     7021 gccatccgaa aaggaaatcc gttttaacgg ttaatcagaa tccaaaatgg gtaaacttcc
     7081 attttagttt tgctgttctg ctggcatcag taaatttctt agtttttcca ttgcaatttt
     7141 gttcgccgga atactgtcta gactctgaat aacgtgtgct ttttccagtt tggtcaggtg
     7201 ccagctcaag gaagtacgct gtttatcgtt ttgtatcata tggaattcaa caatatcaag
     7261 ttttgaattc aaccaagatc ctgcatattg caaaagctga tcgttgtcaa aatcctgtgt
     7321 gtagaaaata ttatcgtaca aatttccaag tggttttacc aatcgtttga ataccgagtt
     7381 tttcacccgg ttttcttcta cttctttttg ccggtctctg atctgcagaa tgacaactcc
     7441 atctgtatta ctgctgatcc agtttctata tacgtaaatg tatcgtaatg caacccggat
     7501 cccatagttg tcgcgatatc ctgcatccat aacttcaatt gccggttcgc ttggaagtgt
     7561 aaccattggc atcacatagg ggaaagaggc actcatacgc aaagccgatg agaattttaa
     7621 attccaagcg ttctgatcct ggaatagttt ggtgaattct acatattcct gtccgggtaa
     7681 ttcgtcaatt tctacatccg gacggcaatt aacaagatgt gaaaccggtt gagcagatat
     7741 aataaggcga cgtccatcgt taataatagt aggacaaaac atcatggttg gaatttttga
     7801 tgttttttcc ggtacgatgt attcgtaaag tcgtttattg aataccgaat ccaaattctg
     7861 gttcatgtat ttttcgaaca tgtatccacg gtcaatcgtg taggagtagg gg
//
LOCUS       DAOMYG010000094         7899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3052_length_7899_cov_8.780342, whole genome shotgun sequence.
ACCESSION   DAOMYG010000094 DAOMYG010000000
VERSION     DAOMYG010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3052_length_7899_cov_8.780342"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1692)
                     /locus_tag="PK610_04385"
     CDS             complement(<1..1692)
                     /locus_tag="PK610_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRJ35198.1"
                     /translation="MFEKIIALSMRNPLMVFILVGAIIAGGLWSLRNIAIDAVPDITN
                     NQVQVVTSTPSLAAPEVEQVITFPLENKLRNIPNVEEVRSISRFGLSVITVVFREEVP
                     QLDARQLVKEQIDIAGAEIPASIGVPELMPITTGLGEIYQYVLKVDKGFEDRYNDMEL
                     RTIQDWVVKRQLSGTPGIIEISSFGGRVKQYEVAVDPSLLRQHELTIAEVFDALENNN
                     ENSGGSYIERGPNAYYIRSYGRVESKDDIEGIVIKSKAKNPLRIRDIGTVEWGSPNRF
                     GAMTMDGKGEVVGGIVLMLKGANSSEAIENVHRSVAEIQRTLPEGISIYGYLDRSVLV
                     GKTISTVSKNLIEGGLIVVFVLVLMLGNFRAGLIVASVIPLSMLFALILMKYFGVSAN
                     LMSLGAIDFGIVVDGAVIIVESVLHVLHVKYAGQTLSRSKFREVVGHSAQGILQSAAF
                     GVLIILIVFVPILSLSGIEGKMFRPMAQTVSFALLGALLLSLTYVPVIAALTLSRKIH
                     SGEGFSDRMIGFFKRIYQPALERVLRQPKTTISMAFLVFVFSIGVFSQMGGEFIPT"
     gene            complement(1752..2117)
                     /locus_tag="PK610_04390"
     CDS             complement(1752..2117)
                     /locus_tag="PK610_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35199.1"
                     /translation="MIRNVFIILSSLIVLLHNLVPHDHHSDFGNMEDHELHAVSDDLI
                     DPLLLVFHEDLSGSLDNVLLKEKKNDVQPVFYLLPTFVCFVVNNQPLTLTIQPFSFFS
                     EREITGHYYHHLLRGPPAC"
     gene            complement(2198..3658)
                     /locus_tag="PK610_04395"
     CDS             complement(2198..3658)
                     /locus_tag="PK610_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRJ35200.1"
                     /translation="MNKKFIFGIAGPLLGIITHFWLVQRGLDPAQGKAAALLVWMATW
                     WVSEAVNIYITALLPIVFLPVCGVLKMEEVAPNYMHEIIFLFIGGFLFAYALEKWGLH
                     RRIALRIILSVGKTPASVLFGVMFAAYFLSMWINNTATTAMLLPAVMAIGVQLERDER
                     LEGITAPLLIGLGFAASIGGMATLIGTAPNMIFLKEYTSAFPNGTSIGFATWMMFGIP
                     ISIVIFLGCYYALRFQFRKVMIKESIDLTRCHSEYHALGKTSYEERWMLFFFIMAILM
                     WFFLSELKFGSLTIPPWTSLFGLPPKMIVESTVAMIIAFLLFFIPSRDGKDTLLSFAE
                     VRRLPIGIIFLFGGGFAISKAVVSTGLDQSMAGALSGVAHLHPVLIVLILAVFMTLLS
                     EFASNTASLQLVFPVLVSFVGALPVDPLLVLIPVTLAASCGFMLPIATPPNTIVFGSE
                     RITAGQMIKAGFMVDLVGIIVITISAFTLIALVLGV"
     gene            complement(3658..4749)
                     /locus_tag="PK610_04400"
     CDS             complement(3658..4749)
                     /locus_tag="PK610_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HRJ35201.1"
                     /translation="MPKFSNQPDFEMRVIVVLCFAMLLSNPVFAQKKSKKMGAKFVLV
                     IHGGAGTILKENMTPEKEKAYMDKLTEALIAGYKVLKDSGTSIQAVQAAIMIMEDSPL
                     FNAGKGSVYTNDERIEMDAAIMDGKTGKAGSVAGVRTVKNPITAAIAVMNRSEHVLLT
                     GEGADAFAKKSGCTIVDTSYFFDLNRLQQLRKVKEQEKAVLDHDGEQGMHTDSSAIEF
                     NVDLNPDKKFGTVGAVALDVHGNLAAGTSTGGMTNKRYGRVGDVPIIGAGTYADNATC
                     AISCTGHGEYFIRNVVAYDVAALMKYKGWSLEKASHHVVMEKLASLKGMGGLIAVDRE
                     GNYTMPFNTPGMYRGAIRADGKVEVYIYR"
     gene            4733..5518
                     /locus_tag="PK610_04405"
     CDS             4733..5518
                     /locus_tag="PK610_04405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ35202.1"
                     /translation="MLNLGIFWKYLKYRLRSKTRYDVHSPFLFAFIEEVLKNKGNYYA
                     YHKIEPLRRKLLKDKRIIQINDLGAGSKTSKGSVRKVRSIAQTALTPPKRAQLIHRIA
                     VKCKAKTSIELGTSLGITTLYLATANSSGQVITFEGSSKIRKIALEQFNAAGVKNIRS
                     IPGNFDSSLPEYLKENPIIDLALIDGNHRMEPTLRYFNQILKNCHNDSILIFDDIHWS
                     EEMESAWETIVKDDRVTLTLDIFFFGIVFLKKEVQKEHYILRY"
     gene            5627..6175
                     /locus_tag="PK610_04410"
     CDS             5627..6175
                     /locus_tag="PK610_04410"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRJ35203.1"
                     /translation="MKIYTKTGDKGQTSLIGGTRVLKSSLRIEAYGTVDELNSWIGVL
                     RDHDTSHTHTATLLEIQDRLFTIGAILASEPGKSKMKIPELHDTDVEFLEAGIDALNE
                     SLPEMKSFVLPGGHPSVSFCHVARCVCRRSERICVDLSQNEEVAEIVIRYLNRLSDYL
                     FVLSRKLSQDQHAIESPWKPRM"
     gene            6305..6526
                     /locus_tag="PK610_04415"
     CDS             6305..6526
                     /locus_tag="PK610_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HRJ35204.1"
                     /translation="MYWTLELASYLEDAPWPATRDELIDYALRAGAPMEVVENLQEME
                     DEGEIYESIEEIWPDYPSKEDFFFNEDEY"
     gene            complement(6593..7726)
                     /locus_tag="PK610_04420"
     CDS             complement(6593..7726)
                     /locus_tag="PK610_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35205.1"
                     /translation="MKKLVYLFLLPVVIAACSSEPSKDTEKSDSNVLVQEDIYNFDKV
                     EAFAATVADSVREASRKVFLNGIDLYRNQKQPEKAIAEFRKAILMCPDAKSYYELGNA
                     LMDIRNYEEAISSYHIAEQLNYHPLSKVMYNLACAYSMTENGEEGLRYVRLAIENGYA
                     NSKHLLSDTDLSFVRGLPDFKGVYEEAMSGVSSPEAALFDLFMVGFPDSQIPLIVKAE
                     ESQKIKLINSIAYDFELFVPQMVNPSFSREVGDEFYYLAKINETEKYVALMYAGVQVM
                     YDTPPVYHILMTYDHNGKVIDKLEVGGFHYHDEPLKGYKIGKDMSVEVSEYQMVYEKS
                     VDEFGYDNNPVSASEFLSKRTFAIQENGKIKQTSGEPMAFWRR"
BASE COUNT         2512 a         1775 c         1482 g         2130 t
ORIGIN      
        1 cgtaggaata aactctcctc ccatttggga aaagactcca attgaaaaaa caaacacaag
       61 aaatgccatt gaaattgttg ttttaggttg gcgaagaact ctttcaagag cgggttgata
      121 gatgcgttta aaaaacccaa tcattcgatc agaaaaccct tctccggaat gaattttcct
      181 acttaaagtc agtgctgcaa ttaccggtac atatgttaaa cttaataaca gagcgcccaa
      241 caaggcaaaa ctcactgttt gtgccattgg acgaaacatt tttccttcaa taccggaaag
      301 tgacaggata ggcacaaaca caatgagtat gatcaatact ccaaatgcag cagattgaag
      361 aataccctga gctgaatggc caaccacttc cctgaattta cttctgctca gcgtttgccc
      421 tgcatatttt acatgcaaaa catgcaacac gctttcaacg atgatcactg caccgtccac
      481 cactattccg aaatctattg cacccagcga catcagattt gcagacactc caaaatactt
      541 catcaggata agcgcaaaca acatactcag cggaatgacc gaagcaacaa tcaaacctgc
      601 gcgaaaattc cccaacatga gcacaagaac gaatacaaca attaatcccc cttcaatgag
      661 atttttactt acggtagaaa ttgttttccc cacaagaaca gatctgtcga gataaccata
      721 aatagaaatt ccttccggca acgtgcgctg aatttctgca acactacggt ggacgttctc
      781 aattgcctcc gaagaattgg caccttttaa catcagaaca attccaccaa ccacttcacc
      841 ttttccatcc atggtcatcg ctccgaaacg attcggagaa ccccattcaa cagttccgat
      901 atcacgaatc cgtaatggat tttttgcctt tgatttaatt acaattcctt caatatcgtc
      961 cttcgattct acccgaccat aacttctgat gtaataggcg ttaggtcctc gttcaatata
     1021 actaccacca ctgttttcgt tgttgttttc gagcgcatca aaaacttcag caatggttag
     1081 ctcatgttga cgcagcaaag acggatcaac tgcaacttca tattgtttta cacgaccacc
     1141 aaaagagcta atctcaataa ttccaggagt gcccgacaat tgccgcttca ccacccaatc
     1201 ctgaatggta cgaagctcca tatcgttgta ccgatcctca aaacccttat ctactttcaa
     1261 aacatattgg taaatctctc ccaaccctgt tgtaatgggc attagttcgg gaacaccaat
     1321 tgaagctgga atttcagccc ctgcaatatc tatttgttct tttaccaatt gacgagcatc
     1381 caattgtgga acttcttcac ggaataccac agtaatgact gaaagtccaa atcggctaat
     1441 tgaacgaacc tcttcaacat tcggaatgtt tcttaattta ttctcaagcg gaaaggttat
     1501 aacctgttcc acttcaggag cagcaagcga aggtgtactt gttactacct gaacctggtt
     1561 attggtgata tccggcactg catcgatagc aatatttcgg agcgaccaca aaccacctgc
     1621 aataatagca cctacgagta taaataccat caagggatta cgcatggaaa gcgcaatgat
     1681 cttttcaaac atagaattgg aaattaaaat tggaaatgaa tcctaattgg attcttagtt
     1741 aaacaaaatg cttaacaagc aggaggtccc ctgagtaaat gatgatagta atgaccagtg
     1801 atttcccttt cagaaaaaaa cgaaaacggt tgaatcgtaa gagtcaatgg ttgattgttg
     1861 actacaaaac aaacgaatgt gggcagcaaa taaaataccg gctgaacatc atttttcttt
     1921 tctttaagaa gcacattgtc caacgatcct gaaagatcct catgaaaaac gagcaacaaa
     1981 ggatcgatca gatcatcaga aactgcatga agttcatgat cctccatatt accaaaatcc
     2041 gaatggtgat catgcggaac cagattgtgc agcagcacaa ttaaagatga caatatgata
     2101 aacacgttgc gaatcacatt tcaaatttag gataaatgat taacatatca ggtaacatct
     2161 atcacactat gacaattatc agcagccctg ttgaatacta aaccccaagt accaaggcta
     2221 tcagggtaaa ggctgagatg gtaattacaa tgataccaac caaatccacc ataaatccag
     2281 ccttaatcat ttgtccggcc gttattcgct cgctaccgaa aacgatggta tttggtggtg
     2341 ttgcaatggg taacataaac ccacaggaag ccgccaaggt aacaggtatc agcacaagca
     2401 aaggatcaac cggaagcgca ccgacaaagg aaaccaggac aggaaagacg agctgcaggg
     2461 aagccgtatt ggatgcgaat tcggaaagca gggtcataaa cacagcaaga atcaacacga
     2521 ttaataccgg gtgcaaatgt gcgacacctg ataatgctcc tgccattgac tgatccaggc
     2581 cggtactgac aacagctttg gagattgcaa atccaccacc aaacagaaaa ataattccaa
     2641 tgggtaatct gcgaacctca gcaaatgaaa gtaacgtatc ttttccatcg cgcgagggaa
     2701 taaaaaacag cagaaaggca atgatcattg caaccgtaga ttcaacgatc atttttggtg
     2761 gtaacccaaa taggcttgtc cacggaggaa tagtaagtga tccaaatttt aattccgaaa
     2821 gaaaaaacca catcaagatt gccataataa aaaagaaaag catccaccgt tcttcatagg
     2881 acgttttccc taatgcatgg tattctgaat ggcagcgtgt gagatcaata ctttctttta
     2941 tcatcacttt gcgaaattga aatcgcaagg cataataaca acccagaaaa atcactatac
     3001 taatcggaat accaaacatc atccatgtgg caaacccgat tgatgtccca ttgggaaatg
     3061 cagaggtata ttcttttaga aagatcatat tgggtgctgt tccgattaga gtagccattc
     3121 ccccaataga tgcagcgaag cccaaaccta taagaagtgg ggccgtaatt ccttcaagac
     3181 gctcatccct ttcgagctga accccgatag ccattactgc aggaagcaac attgcagttg
     3241 tagctgtgtt gttgatccac atggacaaaa agtatgctgc aaacattact ccgaataaaa
     3301 cggaggcagg tgtttttcca acagacagaa ttattcgcaa ggcaattctt ctgtgtaaac
     3361 cccatttttc gagtgcgtag gcaaataaaa atcctccaat gaaaagaaaa ataatctcat
     3421 gcatataatt gggcgccact tcttccattt tgagcacacc acatacggga agaaaaacaa
     3481 ttggcaatag agccgtgatg taaatgttta cagcttcgct aacccaccag gtagccatcc
     3541 acaccaataa tgccgccgct tttccctgtg caggatccaa tccgcgctgt accagccaga
     3601 aatgagtaat gattccaaga agcgggcctg caattccgaa aataaatttc ttattcatca
     3661 gcggtaaata tatacttcaa ctttaccatc tgcgcgaatc gcccctctat acataccggg
     3721 agtattaaac ggcatggtat aattcccttc cctgtcaact gcaattaatc ctcccattcc
     3781 tttaagggaa gccaactttt ccattaccac atgatgggat gctttttcga gcgaccatcc
     3841 tttgtacttc atcaaggcag caacatcata agcaactaca tttctgataa aatattcgcc
     3901 gtgaccggta catgatattg cacaggttgc attatcggca taggtaccgg ctccaattat
     3961 gggaacgtct cctactctgc cgtaacgttt gttggtcata cctcctgttg acgttcctgc
     4021 agcaagattt ccgtgtacat caagtgcaac cgcaccaact gtcccaaatt ttttatcggg
     4081 attcaaatca acattaaatt caatggcaga agagtcggta tgcataccct gctcaccatc
     4141 gtggtcgagc actgcctttt cctgctcttt cacctttcgc aattgctgaa gtcggttcag
     4201 atcaaaaaaa taacttgtat ccactattgt acatcctgac ttttttgcaa atgcatctgc
     4261 gccttctccg gtaagtaaaa catgctcact cctgttcatc acagcaattg cagcagtgat
     4321 cggatttttc actgtacgaa ctcccgcaac agatcctgcc tttccggttt taccatccat
     4381 tatggcggca tccatttcaa ttcgttcatc attggtataa accgagcctt tacctgcatt
     4441 gaacaaagga ctgtcttcca taatcataat tgcagcctga actgcctgaa tagaagttcc
     4501 gctatccttt agcaccttgt aacctgcaat taatgcttcg gttaatttat ccatgtatgc
     4561 tttctctttc tcaggagtca tattctcctt taaaatggta ccggcgccac cgtgaataac
     4621 aagtacaaat tttgctccca tcttcttgct ctttttctgt gcgaaaacag gattacttag
     4681 taacattgca aaacacaaga ctacaattac tctcatttca aaatcgggtt gattgctaaa
     4741 cttaggcatt ttttggaaat acctcaagta ccggcttcgt tcaaaaacga ggtacgatgt
     4801 gcattctcca tttcttttcg catttattga agaagtatta aaaaacaaag gcaattatta
     4861 cgcctatcac aagattgaac cgctgaggag aaagctcctg aaggataaaa ggatcataca
     4921 gatcaatgac ctaggtgcag gatccaaaac aagcaaggga tctgttcgca aagtgagaag
     4981 tattgcacag actgcattaa ctcccccaaa acgcgctcag ctgatacatc gtattgctgt
     5041 aaaatgtaag gcaaaaacga gtattgaact tggaacctcc cttggaataa caacacttta
     5101 tttggcaaca gcaaattcct caggacaagt aatcaccttc gaaggaagtt caaaaatccg
     5161 gaaaattgcg cttgaacaat tcaatgctgc tggagtcaag aacattcgtt ccattccggg
     5221 aaattttgat tccagtttac cggagtattt aaaagaaaat ccaatcattg atcttgcgct
     5281 tatcgatggc aatcaccgta tggaacccac cctgcggtat tttaaccaga tcctgaaaaa
     5341 ttgtcacaac gacagcattc tgattttcga tgatattcat tggtctgagg agatggagtc
     5401 ggcctgggaa actattgtca aagatgatcg cgttacactt acactcgaca ttttcttttt
     5461 cggaattgta tttctgaaaa aagaagttca aaaggaacat tacatactcc gctattaatt
     5521 caaaataaac cattactcca ttctgattgt tcaatggtag tcaaatgcag gaaaaatgaa
     5581 gatgatggtt aatccttatc tttggtaaaa tcaatcagga aagcctatga aaatttacac
     5641 gaaaacaggt gataaagggc aaacatccct aatcggaggt acccgtgtat tgaagtcgag
     5701 tttgcgaatt gaagcatacg gcacggttga tgaattgaat tcctggattg gagttttgcg
     5761 tgatcatgat acttctcaca cgcatacagc aacattactg gagatacagg accggttgtt
     5821 taccatcggt gctattttag cgagcgaacc cggaaaatcg aaaatgaaaa tcccggaact
     5881 gcacgataca gacgtcgaat ttcttgaagc aggaattgat gctttgaatg aaagcttacc
     5941 ggaaatgaaa agctttgtat tgcccggtgg acacccctct gtttccttct gccatgttgc
     6001 ccgttgtgtt tgccgtcgtt cagaacgcat ctgcgtggac ctctctcaaa acgaagaagt
     6061 agccgaaatt gtgatccgat atctgaaccg tttatcggat tatctcttcg ttctgtccag
     6121 aaaactcagc caggatcagc atgcaattga aagtccctgg aaaccaagga tgtgaaaaac
     6181 aacacgatta aactgtgaat caatgggtta cgcaatgata actccttttg acatttcctg
     6241 atcataatag aacaaaaaat ctactttcgc gtaatcttca aaaatcaaca agaaaggaga
     6301 cgttatgtac tggactcttg aactcgcatc ctacctggaa gacgccccct ggcctgccac
     6361 gcgtgatgaa ttaatcgact atgccttacg tgcaggtgca ccaatggaag ttgtagaaaa
     6421 tcttcaggaa atggaagatg agggtgaaat ctatgaaagc attgaggaaa tctggcccga
     6481 ttatccatcg aaagaggatt tctttttcaa tgaagacgag tattaaatct ttttaatgag
     6541 cataaaaaag ccccgaaaat attcggggct tttttattat aacgcgttga aattatcttc
     6601 tccaaaaagc cataggctca cctgaagtct gtttgatttt tccgttttcc tgaattgcga
     6661 atgtacgctt gctcaaaaac tctgatgcag aaacaggatt gttgtcgtat ccaaactcat
     6721 ccacactttt ttcatacacc atttggtatt cgcttacttc aaccgacata tcctttccga
     6781 tcttataccc tttcaaaggt tcatcatgat aatgaaaacc gccaacctca agtttgtcga
     6841 tcacttttcc gttatgatcg taggtcatga gaatatgata tacaggaggt gtatcgtaca
     6901 tcacctgaac accggcatac attaaagcaa catacttttc ggtttcatta atcttggcca
     6961 gataataaaa ctcgtcacct acttcacgac taaacgaggg gttgaccatt tgcggaacaa
     7021 ataattcgaa gtcgtaggca attgaattga tcaattttat tttttgcgat tcttctgctt
     7081 ttacgataag cggaatctgc gaatcgggaa aaccaaccat gaataaatca aataatgcgg
     7141 cctccggaga ggaaacgccg ctcatggctt cttcatatac ccctttaaag tcaggcaatc
     7201 cgcgtacaaa agataaatca gtatcagaaa gcaggtgttt tgaatttgca taaccgtttt
     7261 caattgcaag acgaacataa cgtaatcctt cttccccgtt ttctgtcatg gaataggcac
     7321 aagccaaatt gtacatcact ttagaaagcg ggtgataatt caattgttct gcaatatgat
     7381 aagaggaaat ggcttcttcg tagttacgga tatccattaa tgcattaccc aattcgtaat
     7441 aggattttgc atcggggcac ataagaatag ctttacggaa ttctgcgatc gctttctcgg
     7501 gttgcttttg attgcggtaa agatcaattc cattcagaaa caccttgcgc gatgcttcgc
     7561 gaacagaatc cgcaacagtg gcggcaaagg cttcgacctt atcgaaattg taaatatcct
     7621 cctgaaccaa aacattggag tcagattttt cggtatcctt tgaaggttca gatgaacacg
     7681 ctgcaattac aacaggcagc aggaaaagat aaactagctt tttcatagat tcatttcagt
     7741 attataaaga taatgcaaag gcaggaggaa tggaacagcg aataataatc aaaaggcaac
     7801 ctttacacat ccaattctgc atttctggct tgaagccctg aaaatgagtg cgaagaattc
     7861 agattacgga tccatttaag aacgagcgta atgcttata
//
LOCUS       DAOMYG010000095         7852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3073_length_7852_cov_11.013210, whole genome shotgun sequence.
ACCESSION   DAOMYG010000095 DAOMYG010000000
VERSION     DAOMYG010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7852
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3073_length_7852_cov_11.013210"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..562)
                     /locus_tag="PK610_04425"
     CDS             complement(71..562)
                     /locus_tag="PK610_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35206.1"
                     /translation="MKNLVLMLLGIGMMATVSAQEPEKKGESHKEKMEAAKIGFITKE
                     LQLTPEEAQKFWPVYNQREAELDEVKKAMRKIHRDKKIEEMSDAEVDKSMEDLMLLRQ
                     RELDIEKKYHTEFKKVLPVKKVAQLYHAEMKFRHEVMKEWKERHGHGEHGQKGQPDKK
                     GSK"
     gene            complement(620..1162)
                     /locus_tag="PK610_04430"
     CDS             complement(620..1162)
                     /locus_tag="PK610_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35207.1"
                     /translation="MNSNSTYQPSNDSLSAPSGYHEEFHRNLMQKLERDEWQNEAPVL
                     ASLNKPRELLAPDNYFAALPELVQNKLSSGKVVSIQSRLWFRLAAAAAIIALVVNFLR
                     PDTNEFSGEISLSEVEQSTLDNYLDVEFSAYLDEDDLAEIVSNENNRSFSFSASTEEL
                     TLYLEEEADTDDMAELFAEN"
     gene            complement(1159..1746)
                     /locus_tag="PK610_04435"
     CDS             complement(1159..1746)
                     /locus_tag="PK610_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ35208.1"
                     /translation="MIEKSDKELLALFLQGDNANKAFSLIVQKHQRKLYAIIRRVLID
                     HDDTNDVLQNTFIKAWNGLSSFREEAQLSTWLYRIASNETLSFLRQSKRDRHLSIDQL
                     YTDSNYSVGNAIGPSAEEIEKKLHHAIQQLPEKQRLVFTMKYYEDLTYEQMSDILGTS
                     VGALKASFHIAAKKIEEEITNGALNLSPANASNNK"
     gene            complement(1775..2728)
                     /locus_tag="PK610_04440"
     CDS             complement(1775..2728)
                     /locus_tag="PK610_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008639158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRJ35209.1"
                     /translation="MEKFKIKNQQDTRSGFGEGLLELGRKNPNVVALCADLTGSLKMD
                     AFEKEFPERFFQTGIAEANMIGLAAGMTVGGKIPYTGTFANFSTGRVYDQIRQSVAYS
                     DKNVKICASHAGLTLGEDGATHQILEDIGMMKMLPGMTVINPCDFNQTKAATIAIAEH
                     VGPVYLRFGRPKVPNFTEENQKFEIGKALMLNEGVDVTIFATGHLVWKAIEAGRILLE
                     KGINPEIINIHTIKPLDQETVLASVRKTKCCVTAEEHQAAGGLGESIAQVLARNFPAP
                     MEMVAVNDTFGESGTPDELMVKYGLDTPDIVRAAERAVARK"
     gene            complement(2733..4112)
                     /locus_tag="PK610_04445"
     CDS             complement(2733..4112)
                     /locus_tag="PK610_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRJ35210.1"
                     /translation="MKRYLFSGILLLFFIAGAQAQTKPEQKTRILFIFDASNSMNYPW
                     QGSSRIAVARQVMLRTIDSLGNIPNLEIALRVYGHQTPLMPGQQDCNDTKLEVPFNGD
                     LQGNIKKIRNTIGYVECRGTTPIARSLEKAGADFPEDKNVRNVIILVTDGIEACDEDP
                     CAVSRALRSKGIIVKPFVIGVGLSLDHLTSLECIGTVYDASTPETFQTILGVVVSQAL
                     NNTTAQININNIQKLPKETNISFTLFDERTGAVKYHYIHSLNKAGNPDTLTLDPLLTY
                     RLVVHSLPTVEKKGIRLTPQKHNTIEIDAPQGSIETKISTYNKYAPVFCVVRKQGESQ
                     TLNAQKFPEKQDYLVGKYDIEVLTLPRIYIRDVIVEQSKVTKIEIPLAGVLDLNTPNG
                     GYGSIFVIEDGKPVWVCDLKDEERKHFINLQPGKYKVVYRNKRSDKTVYSAEKSFTIT
                     SGQTTVISL"
     gene            complement(4114..4962)
                     /locus_tag="PK610_04450"
     CDS             complement(4114..4962)
                     /locus_tag="PK610_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRJ35211.1"
                     /translation="MADTSTLKRTCSQVRRDIVRMVAAVQSGHPGGSLGCAEYFVAQY
                     FHNLKHDPKKFNMDGKNEDLFFLSNGHISPVWYSVLARTGYFPVSELATFRKLNTRLQ
                     GHPATHDHLPGIRMASGSLGQGLSVALGAALTKKLNGDKQLVYSLHGDGELQEGQIWE
                     AALFAAHRKIDNVIATVDYNGRQIDGDVENVLSLGNLRSKWEAFGWTVTEIENGNDID
                     QVINGLDHAKTLTGKGKPVIILMRTEMGMGVDYMMGTHKWHGSAPNAEQTEKALAQLE
                     ETLGDY"
     gene            5134..5514
                     /locus_tag="PK610_04455"
     CDS             5134..5514
                     /locus_tag="PK610_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35212.1"
                     /translation="MRTIALFILFLFAGILAQAQKSDWDQRLESAYTVKELTEMKKSD
                     PSKLAFLNAVATKGWVIMDLPSEKGSATEIRGSITVPDQKNVNLFALNLFPEKTNWQY
                     YRISGTDKMLVVYSEDHIRSQMNK"
     gene            5539..>7852
                     /locus_tag="PK610_04460"
     CDS             5539..>7852
                     /locus_tag="PK610_04460"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ35213.1"
                     /translation="MKNRIIALLILLSPAAMAQTITITDPDYDQANPITCANFDDGSV
                     QNFFDAGGAGGNYGNNQNEVIVICPDINALVGSKVTVAFAINVGFTWDVLAGDFVSVF
                     DGTSTAAPLLGTHNSVTDPNGFTYTASFANPTGCLTIQFTSDASGTGTGWQANVSCGN
                     PPQPYDPHILGFVNGAGPNALNPLDTGYIDICLEDSVMLVAAPNFMYSSENNQLNYGV
                     PFGYSQNNNNSTFLWNFSDGTSQTGDTVWFSPPNRSGYLVTLRITDPVGYIQIMKCKI
                     RVSTIPSFAGTMPVEDTICLGEQTYLIGGVTATDTVGVSPTVGAFEIGGIFAGLTYLP
                     DGSGQNYTTSVTITDFLPGQTITSASDIVSFCVTMEHSYLGDLEMGLTCPNGTMINIF
                     NSYGPGGLFPGGFNGGGTFLGEADDTGNGTPGIGYEYCFEDNAPWMTLGQERAAGNTQ
                     NLTAPPSPSAGQTMPSGTYDPEVSFASFIGCPINGNWTITIRDNLGIDDGYIFEWGIF
                     FNPAINPNTEFYSPLISTEMWQSSPDIISGQGDTAIVVRPTTSGPHFYTFQVTDNFGC
                     TYDTTIRVIVLPVPTLPASGQTCDLFFNVTGTTAPGAVSWSTISQPSGSIVNYSSTTA
                     LNPTITVNQTGEYQFAVTDAICNFSDTITVYYLEDPAPSLPDGTVCSDEFLVLDATEP
                     GSTYLWSTGETTAVITAMSAVPGPVAFTVAVSNLCATIVDTSIVMFEDCSVIIPNVIS
                     PNGDGENDKFVVVGLENNPNSVLEIYDRWGL"
BASE COUNT         1963 a         1721 c         1701 g         2467 t
ORIGIN      
        1 aattagtgaa tctgatgaaa tagtggaagg ccggagcaaa aaaaaggact tcgttaagaa
       61 gtccttttgt tcatttactg ccttttttat ccggttgtcc tttttggccg tgttcaccat
      121 gaccgtgtcg ttctttccac tctttcatta cttcatgacg gaatttcatt tctgcatggt
      181 agagctgagc cacttttttt acggggagta cctttttgaa ttcggtgtgg tacttctttt
      241 caatatcgag ttcgcgttga cgaagcagca tgaggtcttc catggatttg tcgacttcag
      301 catcactcat ttcctcaatt tttttgtcgc gatggatttt gcgcattgct ttttttacct
      361 catccagttc tgcttcgcgc tgattgtata caggccagaa tttctgagct tcttctggtg
      421 taagctgaag ttccttggta atgaaaccga ttttggcagc ttccattttt tctttgtgcg
      481 attcgccttt tttttcaggt tcctgtgcac ttactgtcgc catcatcccg attccgagca
      541 gcatcaatac tagatttttc attgtaagtg atttagatta attcgtttaa taatcgtatt
      601 gtgtttgtat ttatgcattt caattttcgg cgaaaagttc agccatgtcg tccgtatccg
      661 cctcttcttc caggtaaagt gttaattctt cagtactcgc agaaaaagag aatgagcggt
      721 tattttcatt cgatactatt tcagcgagat catcttcatc caggtaggcg gagaattcaa
      781 catccagata attgtcgagt gtcgattgct ccacttcgct gagtgaaatt tcgccggaga
      841 attcattggt atcaggtcga agaaaattca ctacaagtgc aatgatagca gcagctgccg
      901 caagtctgaa ccataagcgc gattgtatcg aaacgacctt gcccgacgaa agtttgtttt
      961 gcaccagctc cggaagtgca gcaaaataat tatccggggc tagcagttca cgtggtttgt
     1021 tcagcgaggc aagcacaggc gcttcattct gccattcatc ccgctccagt ttttgcatga
     1081 gattcctgtg aaattcttca tggtaccctg atggggcgga aagtgaatcg ttggaaggtt
     1141 gatatgtaga attgctgttc atttgttgtt tgatgcatta gccggcgaaa ggtttaatgc
     1201 gccgttggtt atttcctctt ctattttttt tgccgcaata tgaaaacttg cttttaaggc
     1261 cccaaccgaa gtgccaagaa tatccgacat ctgctcgtag gtcaggtctt cgtagtactt
     1321 catggtaaaa accaatcgtt gtttctcagg taattgctga atggcgtggt gaagtttttt
     1381 ttctatttct tcagcactag gaccaatcgc attgccaacg gagtaattac tgtcggtata
     1441 aagttggtcg atggacaaat gtcggtcgcg tttgctttgc cgaagaaaac tcagggtttc
     1501 attactcgca atcctataca gccaggtgct caattgcgct tcttcgcgga aagaggataa
     1561 tccattccag gctttgataa atgtgttttg cagtacgtcg ttcgtatcgt catgatcgat
     1621 caatacgcga cgaataatag catatagctt tcgctggtgc ttttgtacaa tcagtgagaa
     1681 agctttattt gcgttatctc cctgtagaaa caaggccagt agttctttat cgcttttttc
     1741 gatcataaca ctacggccgc tatttccgtg ggccttattt acgggctaca gctctttctg
     1801 ctgcgcgaac aatatcaggt gtatctagtc cgtatttaac catcaactca tccggtgtac
     1861 cgctttcgcc aaaagtgtcg tttaccgcta ccatttccat aggtgcaggg aaattacggg
     1921 ccagcacttg tgcaatgctt tctcccaatc ctcccgctgc ctgatgttct tcggcagtaa
     1981 cacaacattt tgtcttgcgt acagaagcta agacggtttc ctggtccaaa ggtttaatcg
     2041 tgtgaatatt gatgatttca ggattaatcc ctttttcaag cagaatgcgt cccgcttcaa
     2101 ttgctttcca taccaaatgt cctgtggcga aaatggttac atccacacct tcattcagca
     2161 ttagcgcttt tccaatctca aatttttgat tctcttctgt aaaattgggg actttaggtc
     2221 ggccaaatcg gaggtaaacc ggacctacat gttcggctat tgcaatggtg gctgctttgg
     2281 tttgattaaa atcgcaagga ttaatcaccg tcattccggg cagcattttc atcattccta
     2341 tatcttcaag gatctggtga gttgcaccgt cttctcccag tgttaatcct gcatgcgatg
     2401 cgcatatctt tacgttttta tcggaatacg caacgctctg acgaatctgg tcatacacgc
     2461 gtccggttga gaagttggca aatgttccgg tatagggaat ttttccacct acggtcattc
     2521 ctgctgccag tccgatcatg ttggcttctg ctattcctgt ctggaaaaag cgttcaggaa
     2581 attctttttc gaaagcatcc atcttcagcg atccggtaag gtcggcgcat agtgcaacta
     2641 cattcgggtt ttttctccct agctccagga gtccttctcc aaatccgctg cgggtgtctt
     2701 gctgattttt gatcttaaat ttttccatgg tgttataaac tgataacggt ggtttgtccg
     2761 gatgttatgg tgaatgattt ttctgctgag tacacagttt tatcgctgcg tttgtttcgg
     2821 tacacgactt tgtatttccc gggttgaagg ttgatgaaat gtttgcgttc ctcatctttc
     2881 aaatcgcaaa cccacaccgg ttttccatct tctatcacga agatcgaacc atatcctccg
     2941 ttaggtgtgt tgaggtcgag cactcctgca agaggaattt caattttggt cactttactt
     3001 tgctcaacaa ttacatcacg aatataaatt cggggcagtg ttaatacttc tatgtcgtat
     3061 ttgcctacca gataatcttg cttttccgga aatttttgag cgtttaaggt ttgcgattct
     3121 ccttgcttgc ggaccacgca aaatacaggt gcgtatttgt tgtaagtgga aattttcgtc
     3181 tcaattgaac cttgcggagc atcaatttca atggtattgt gcttttgagg cgttaaacga
     3241 attcccttct tctcaacagt tggaagcgaa tgaacaacca aacggtaggt taaaagcgga
     3301 tcaagtgtta gtgtatccgg attccctgct ttgttcagcg aatggatgta gtggtacttc
     3361 acagcccctg tgcgttcgtc gaataaagtg aaactgatat tggtttcttt gggtagtttc
     3421 tggatgttat tgatgttgat ttgtgcagtg gtattgttca aagcctgact aaccacaact
     3481 cccaatatgg tttgaaaggt ttccggtgtg gatgcgtcgt aaactgttcc gatacattcc
     3541 agcgaagtga ggtggtccag gcttaatccg acaccaatca caaatggttt tacaatgatt
     3601 cccttgctgc gtagtgctct tgaaactgca cagggatctt catcgcatgc ttcgattccg
     3661 tcggtgacaa gaattattac gttgcgtaca tttttatctt ccggaaaatc ggcacctgct
     3721 ttttcaagtg agcgtgcaat gggagttgtt cccctgcatt ctacataacc gatcgtgttg
     3781 cgtatttttt taatgttgcc ttgcagatct ccattgaaag gaacttcaag tttggtgtcg
     3841 ttgcaatcct gttgaccggg cataagtggt gtttggtgac cataaacccg caatgcaatt
     3901 tccagattcg gaatattacc tagtgaatcg atggtgcgca acattacctg tcgtgccact
     3961 gcaatgcggc tgcttccctg ccagggataa ttcatggaat tggaagcatc aaaaatgaaa
     4021 aggatacgtg ttttctgttc aggcttggtt tgtgcctgtg cgcctgcaat gaaaaaaagc
     4081 aggagaatgc ctgaaaacag gtagcgtttc attttaataa tcaccgagtg tttcctcgag
     4141 ttgagcgagt gctttttcgg tttgttctgc gtttggtgct gatccatgcc atttgtgtgt
     4201 acccatcatg taatcaactc ccatgcccat ttcagtacgc atcagaataa ttacaggttt
     4261 acccttgcct gtcagggtct tggcgtgatc aagtccgttg ataacctggt cgatgtcgtt
     4321 tccgttttca atttccgtta ccgtccagcc aaaggcttcc catttcgatc tcaggtttcc
     4381 aagagagaga acattttcaa catcaccgtc aatttgcctt ccattgtaat ctactgttgc
     4441 aatgacatta tcgattttgc ggtgcgctgc aaataaagcg gcttcccaaa tctgtccttc
     4501 ctgcaattct ccatcgccat gcagactgta aaccaattgt ttgtcgccgt tcagtttttt
     4561 agttagagca gcacccagtg caacacttaa tccctgaccc aaagaacccg aagccattcg
     4621 gatacccgga aggtgatcgt gcgtggcagg atgtccttgc agacgagtat ttaactttcg
     4681 gaaggtagcc agttcgctta cagggaaata gcccgtgcgt gccaatacgc tgtaccaaac
     4741 cggtgagata tgaccgttgg aaaggaagaa taaatcttca ttctttccat ccatgttaaa
     4801 ttttttcggg tcgtgtttta aattatgaaa atactgagcc acaaagtatt ctgcacatcc
     4861 caatgaacct ccggggtgac cactttgaac tgctgcaacc attcttacaa tatcacgtct
     4921 tacctgcgaa caggttcttt tcagggtact ggtgtctgcc atttgaatcg ggattaaggc
     4981 ctcaaatgta aatgtttgca tagggagcta atccctcggg ttaataactt ttgaaaattg
     5041 ggtgatatcc attctttgca tttaaagagt tcaataaaaa acatgccgta aacattctga
     5101 taaaaaatta actttacaac aaaccaaaac atcatgagaa cgattgcact attcattctt
     5161 ttcctttttg caggaatcct tgcgcaagct cagaaatcag actgggatca acgtcttgaa
     5221 tccgcatata cggtgaaaga attgacggaa atgaaaaaat cagatccttc caaactcgct
     5281 tttcttaatg cggttgccac aaaaggatgg gttattatgg atcttccttc tgaaaagggt
     5341 agtgcaacgg aaattcgcgg ttcaatcact gttcctgatc agaagaatgt taacctgttt
     5401 gcgctcaatc tctttcctga aaagacaaac tggcagtact acaggatttc aggaaccgat
     5461 aaaatgctgg tggtctactc agaagaccat atccgcagtc agatgaataa ataagctata
     5521 ccgaaatcca aaataagcat gaaaaaccgg attattgcac tattgattct gctttcaccg
     5581 gcagcaatgg ctcaaacaat tacaattact gatcccgatt acgatcaggc gaatccaatt
     5641 acctgtgcca attttgacga tggttcagtt cagaatttct tcgatgccgg tggcgccgga
     5701 ggtaattatg ggaataacca gaatgaagtc atagtgatat gccccgatat caacgcattg
     5761 gttggtagca aagtaacagt agcatttgca ataaatgttg gctttacctg ggatgtattg
     5821 gccggtgatt ttgtttctgt ttttgacgga acttctactg ctgctcctct tttaggaacg
     5881 cataacagtg taacagaccc caacggtttt acctatacag ccagttttgc aaatcccaca
     5941 ggatgtttaa ctattcagtt tacttccgat gcctcgggaa caggaacggg ttggcaggcg
     6001 aatgtttctt gcggtaatcc tcctcagcca tatgatccgc atattttagg atttgtaaac
     6061 ggagccggac ccaacgcact gaatcctctc gacactgggt atattgatat ctgtctcgaa
     6121 gattcagtaa tgctggttgc tgctcctaat ttcatgtatt catctgaaaa taatcagttg
     6181 aactatggtg ttccttttgg ttatagtcag aacaataaca acagcacatt cctctggaat
     6241 ttttcggatg gtacttctca aaccggagat acagtttggt tttctcctcc taaccgctct
     6301 ggttatcttg taacgctgcg tatcaccgat cctgttggtt atatccagat catgaaatgt
     6361 aagatccgtg tatcaaccat accaagtttt gctggtacaa tgcctgtgga agacacgata
     6421 tgccttggag aacaaacgta tcttattggt ggtgttaccg ctactgatac tgttggtgta
     6481 tctccaactg ttggtgcttt tgagatcggt ggaatttttg caggtttaac ctatctgccg
     6541 gatggttcag gacagaatta tacgacatct gttaccatca ccgatttcct tcccggtcaa
     6601 accattacct ctgcatcaga tatcgtttca ttttgcgtta ccatggagca ttcctattta
     6661 ggtgacctcg aaatgggact tacctgccca aatggaacta tgatcaacat cttcaacagt
     6721 tatggtcccg gtgggttgtt tcccggcgga tttaatggtg gaggtacttt tcttggtgaa
     6781 gccgatgata ccggaaacgg aacacctggt attggttacg aatattgctt tgaagacaat
     6841 gctccctgga tgaccttagg ccaggagcgt gctgcaggta atacccagaa tcttacagca
     6901 cccccaagtc cctctgccgg acagaccatg ccttcgggga catacgatcc tgaagtatcg
     6961 tttgcaagtt ttattggttg tcccatcaat ggaaactgga caattaccat tcgtgataat
     7021 ttgggtatcg atgacgggta tattttcgag tggggaatct tctttaatcc tgcaattaac
     7081 cctaatacag aattctattc tcctttgatt tcaacagaaa tgtggcagtc gagccccgac
     7141 attatttccg ggcaaggtga tactgcaatt gttgtgcgtc ctaccacttc aggaccacat
     7201 ttttatacgt tccaggttac ggataatttt ggttgcacat acgatacaac tattcgtgta
     7261 attgttttac ccgttccaac cttaccagca agcggacaaa cctgcgacct tttctttaac
     7321 gtaacaggaa caacagcacc tggcgcagtg agttggtcaa cgatttcaca gccttccgga
     7381 agtattgtga attattcgag cactacggcc ttgaatccta caataacggt gaaccaaacg
     7441 ggcgaatacc agtttgcagt taccgatgca atttgcaatt ttagtgacac aattacggtc
     7501 tattacctag aagacccggc cccaagtttg cctgacggaa cggtttgttc agatgagttt
     7561 ttagttctcg atgcaacaga acccggaagt acctacctgt ggagcaccgg cgaaactaca
     7621 gcagtgataa cagcaatgag cgcggtgcct ggtcctgttg cttttacagt agcagtgtcc
     7681 aatttatgcg caaccatcgt tgatacttcc attgttatgt ttgaagattg cagtgtaatc
     7741 attccaaacg tgatctctcc aaatggcgat ggtgaaaacg ataagtttgt tgtggttggc
     7801 cttgaaaata acccgaattc tgtccttgaa atctacgatc gctggggatt aa
//
LOCUS       DAOMYG010000096         7843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3076_length_7843_cov_10.909348, whole genome shotgun sequence.
ACCESSION   DAOMYG010000096 DAOMYG010000000
VERSION     DAOMYG010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7843
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3076_length_7843_cov_10.909348"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..586
                     /gene="nusG"
                     /locus_tag="PK610_04465"
     CDS             35..586
                     /gene="nusG"
                     /locus_tag="PK610_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRJ35214.1"
                     /translation="MGEITKKWYVVRAVSGKEKKVRDQVELEMNRLGYSDLVPQVLIP
                     TEKIYQIRNGKKVSKERSYLPGYVLIEATLTGEIVHAIKNLSNVIGFLGAEKGGDPVP
                     LRASEVSRILGKYDELSESEEEINIPYIVGESVKVIDGPFNNFTGVIEEINEEKKKLK
                     VMVKIFGRKTPLELNYMQVEKEN"
     gene            613..1050
                     /gene="rplK"
                     /locus_tag="PK610_04470"
     CDS             613..1050
                     /gene="rplK"
                     /locus_tag="PK610_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRJ35215.1"
                     /translation="MAREITGFIKLQVKGGAANPSPPIGPALGSKGVNIMEFCKQFNA
                     RTQEKAGKVLPVVITVYSDKSFDFIVKTPPVAIQLIEAAKIKSGSAEPNRKKVGSVSW
                     DQVRKIAEEKMPDLNCFTVESAMSMVAGTARSMGLTVSGDKPF"
     gene            1062..1757
                     /gene="rplA"
                     /locus_tag="PK610_04475"
     CDS             1062..1757
                     /gene="rplA"
                     /locus_tag="PK610_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004916771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRJ35216.1"
                     /translation="MARMTKKQKEVAGKYDLEKVYALDDASKIVKEITTTKFDASVDL
                     AVRLGVDPRKANQMVRGTVSLPHGTGKNVRVLVLCTADKEKEAKDAGAEFVGLDDYID
                     KIKGGWTDVDVIITMPSIMGKVGALGRVLGPRGLMPNPKTGTVTQEIGKAVTDVKAGK
                     IDFKVDKTGIVHASIGKVSFDAQKIADNANELIQTLLKLKPSSAKGTYVKSIFMSSTM
                     SKGVEIDTKSVNI"
     gene            1776..2309
                     /gene="rplJ"
                     /locus_tag="PK610_04480"
     CDS             1776..2309
                     /gene="rplJ"
                     /locus_tag="PK610_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRJ35217.1"
                     /translation="MTREQKNQAITELTDLIKANKVMYLADTSSLDAEATHKLRKECF
                     NKKIQMKVVKNTLLQKAMERVEEVDYSPIYAALKGNTALMFSDTGNLPARLIKDFRKG
                     QKGVERPILKGAYVEESIYVGDNMLDTLCNIKSKNELLGDVIALLQSPAKNVISALQS
                     GKHTIAGLVKTLQEKNN"
     gene            2349..2723
                     /gene="rplL"
                     /locus_tag="PK610_04485"
     CDS             2349..2723
                     /gene="rplL"
                     /locus_tag="PK610_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRJ35218.1"
                     /translation="MADVKALAEQLVNLTVKEVNELAQVLKDEYGIEPAAAAVAVAAP
                     GAGGGAPAEEKTSFDVILKNGGANKLAVVKLVKELSGLGLKEAKDLVDGAPGTLKEGV
                     SKEEAEQLKAKLVEAGAEVEIK"
     gene            2845..6657
                     /gene="rpoB"
                     /locus_tag="PK610_04490"
     CDS             2845..6657
                     /gene="rpoB"
                     /locus_tag="PK610_04490"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRJ35219.1"
                     /translation="MATTGKKSKRISFAKSKSTFDYPDFLDIQLKSFQEFFQLETTPE
                     NRENEGLFKVFAENFPITDTRNQFVLEFLDYFIDPPRYTIEECIERGLTHSVPLKAKL
                     KLYCTDPEHEDFETIVQDVYLGTIPYMTPKGSFVINGAERVIVSQLHRSPGVFFGQSR
                     HANGTKLYSARIIPFKGSWIEFATDINNVMYAYIDRKKKLPVTTLFRAIGYQSDRDIL
                     EIFNLADEIKVSKKALKEVVGRRLAARVLRTWIEDFVDEDTGEVVSIERNEVLIDREV
                     ILEDQHIDLIVDSGVKSIILHREEVNSGDFSIIYNTLQKDTSNSEKEAVEYIYRLLRN
                     AEPPDEETARSVIDKLFFSEQRYDLGEVGRYRINKKLGLNMDPGTRVLTKVDIIAIVK
                     YLIELINSKTDVDDIDHLSNRRVRTVGEQLYNQFGVGLARMARTIRERMNVRDNEVFT
                     PIDLINAKTLSSVINSFFGTNQLSQFMDQTNPLAEITHKRRLSALGPGGLSRERAGFE
                     VRDVHYTHYGRLCTIETPEGPNIGLISSLCVFAKVNRLGFIETPYRRVVEGKVLLGED
                     PIYLSAEEEDNRVIAQANADIDTGGKFQGDKVIARLEADFPVVEPKDVALMDVGTNQI
                     ASIAASLIPFLEHDDANRALMGSNMQRQAVPLLRPEAPIVGTGIEELVARDSRVLINA
                     EGEGVVEYVDANQITIRYERTDDERFVSFDGDTKTYNLTKFQKTNQSTSINLKPIVKK
                     GDKVSRGQVLCEGYATKDGELALGRNLKVAFMPWKGYNFEDAIVISERVVREDIFTSI
                     HIDEYILEVRETKRGLEELTADIPNVSEEATRDLDESGMIRIGAEVKEGDILIGKITP
                     KGETDPSPEEKLLRAIFGDKAGDVKDASLRVGPSVKGVVIDKKLFSRAIKDRKMKAQE
                     KPIVDQLDKEYEKEVADLRNVLVEKLLQIVGEEKSAGVFNNFKEEIIKKGVKFSSKAL
                     FAIDYSTINPTNWTSVEKMNKMINRLIHNYNIKANDLLGIYKRKKFQLTVGDELPTGI
                     VKLAKVYLAQKRKLRVGDKMAGRHGNKGVVAKIVREEDMPFLADGTPVDIVLNPLGVP
                     SRMNLGQIYETVLGWAGLKLGMKFATPIFDGAHIDEINKLTDEAGVPRYGRSYLHDGG
                     TGVRFDQPATVGVIYMIKLSHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEME
                     VWALEAFGAANILQEILTVKSDDVVGRAKAYESIVKGENLPQPGIPESFNVLMHELRG
                     LGLNITLE"
     gene            6685..>7843
                     /locus_tag="PK610_04495"
     CDS             6685..>7843
                     /locus_tag="PK610_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRJ35220.1"
                     /translation="MAFRKENKVKSDFDKIVISLASPEMILERSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIS
                     LVVPVAHIWYFRSLPNKIGYLLGLPSKKLDQVIYYERYVVVQAGIATAADGAQLNMMD
                     FLTEEEYLDILDKLPKENQYLDDNDPNKFIARMGADALQELLRRINLEELSYELRHKA
                     NTETSQQRKSEALKRLQVVQAFRDSNERIVNKPEWMVIKVVPVIPPELRPLVPLDGGR
                     FATSDLNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAVDSLFDNSRKSSAVK
                     TESNRPLKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELKLHECGLP"
BASE COUNT         2283 a         1684 c         1874 g         2002 t
ORIGIN      
        1 atgatatttt taaataaata atgcaccggt aacgatggga gaaattacca aaaaatggta
       61 cgtcgtacgc gctgtctccg gcaaggagaa aaaagtgcgt gatcaggtcg agctggagat
      121 gaacaggctg ggttattctg accttgtgcc tcaggtactc attcctaccg aaaagatcta
      181 ccagatccgc aatggaaaaa aagtgagcaa ggaacgcagt taccttccgg ggtatgtgct
      241 gatcgaagct acacttaccg gcgagatcgt gcatgctata aagaacctgt cgaatgtaat
      301 tggctttctt ggcgcggaaa aaggaggaga tcctgttccg ctgcgcgctt ccgaagttag
      361 ccgcattctc ggtaaatacg atgaactcag cgaaagcgaa gaagagatca acattcctta
      421 catcgttggc gaatctgtta aagtaattga cggtccgttc aacaacttca ccggagtgat
      481 cgaagagatc aacgaagaga agaaaaagtt gaaagtaatg gtgaagattt ttggtcgcaa
      541 gacacctctc gagctgaact atatgcaggt tgaaaaagaa aactaatgtt taacaaaaac
      601 taatagctgg caatggcaag agaaattacc ggctttatca agctacaggt gaaaggcggc
      661 gcagccaatc cttcaccacc aatcggtccc gctctcggtt ccaagggtgt taacatcatg
      721 gaattttgca agcagttcaa tgctcgcacg caggagaagg ccggaaaagt gttgccggtg
      781 gtaatcaccg tctacagcga caagtcgttc gactttatcg taaaaacccc tccggtagca
      841 attcagctaa tcgaggcggc aaagatcaag tcaggttccg ctgagcccaa ccgtaagaaa
      901 gttggatctg tttcatggga ccaggttcgt aagatcgctg aagaaaagat gcccgacctg
      961 aactgtttca ctgttgaatc agcaatgagc atggtggcag gaacggcacg cagcatggga
     1021 ttaactgtaa gcggtgataa acccttctaa aaagttgtgt tatggctcgt atgactaaaa
     1081 aacaaaagga agttgcagga aagtatgacc ttgaaaaggt gtacgcactg gatgacgctt
     1141 ccaagatcgt taaggagatc accacaacga aattcgatgc atctgttgat ctcgcagttc
     1201 gtctcggtgt tgatccgcgt aaagccaatc aaatggtacg cggaaccgtt tctctccctc
     1261 acggtacagg taagaatgtt cgcgttcttg tgctttgtac tgcagacaag gagaaagaag
     1321 cgaaagatgc cggtgcggaa ttcgtgggac tcgatgatta catcgataaa atcaaaggcg
     1381 ggtggacgga tgttgatgtt atcatcacca tgccctccat catgggtaaa gtaggtgcac
     1441 ttggacgtgt gcttggtcct cgcggactaa tgccaaatcc aaagacaggt accgttactc
     1501 aagaaatcgg aaaggctgtt acagacgtta aggctggtaa gatcgacttt aaagttgata
     1561 agaccgggat cgttcatgct tccattggca aagtttcgtt cgacgcacag aagatcgcag
     1621 acaatgcaaa tgaattgatc cagactcttc tcaagctgaa gccctcttct gcaaaaggaa
     1681 cttacgtgaa gagtatcttc atgagcagta ccatgagcaa gggcgtcgag atcgatacga
     1741 aatcagtcaa tatctaaccc ttatcccaga aaactatgac aagggaacaa aaaaatcagg
     1801 caatcacaga actgaccgac ctgatcaagg ctaacaaagt aatgtacctg gccgacacat
     1861 catctctcga tgccgaagca acacataagc ttcgcaaaga gtgcttcaac aagaagattc
     1921 agatgaaagt cgtgaagaac acattgcttc agaaagctat ggaaagagtc gaagaagtag
     1981 attactctcc tatttatgca gcacttaagg gcaacactgc acttatgttc tcagacacag
     2041 ggaaccttcc tgcgcgttta atcaaagatt tccgcaaagg acaaaaaggt gttgagcgtc
     2101 ctattctgaa aggtgcttat gttgaagaat caatttatgt tggagacaac atgcttgata
     2161 cactttgcaa catcaagtcc aagaacgaat tgctcggcga tgttatcgct cttcttcagt
     2221 cacctgccaa gaatgttatc tctgctcttc agagtgggaa acacactatc gcaggactgg
     2281 ttaagacact gcaagaaaaa aacaactaat caatcaatcg ttaaacccca atttaaatca
     2341 atagaaaaat ggcagacgtt aaagctctcg ctgaacagtt agttaatctt actgttaaag
     2401 aagtgaatga actcgctcaa gtactgaaag acgagtatgg tatcgagcct gctgcagcag
     2461 cagtagcagt agcagcacct ggtgcaggtg gtggtgcccc cgctgaagag aaaacatctt
     2521 ttgatgtgat ccttaagaac ggtggagcta acaaactagc tgttgttaag ctggtgaagg
     2581 aactctccgg tcttggtctg aaagaagcga aagacctggt tgacggtgct cctggtaccc
     2641 tcaaagaagg tgtctcaaaa gaggaagctg agcaactcaa ggctaaactc gttgaggctg
     2701 gagcagaggt tgagatcaag taatccgctc tattacaata cagtggttaa ggcaccggtt
     2761 tttccggggc cttaaccttt ttgtgttttc agcaggttga atggtcaaac ggtcttggtt
     2821 cttcattttt taaaacaaaa tcccttggca actacaggta aaaagtcgaa acgcataagt
     2881 ttcgcaaaaa gtaaaagcac tttcgattat cccgattttc tggatatcca gttaaagtcg
     2941 tttcaggagt ttttccaatt ggaaactaca cctgagaaca gagaaaacga aggtctgttt
     3001 aaggtattcg ccgagaattt cccgattacg gacaccagaa atcagttcgt actggaattc
     3061 ctcgactact tcatcgatcc tccacgttac accattgagg agtgtatcga gcgcggtctc
     3121 actcacagtg tgcccctgaa ggccaaatta aagctctatt gtaccgatcc ggagcacgaa
     3181 gatttcgaaa ctatcgtgca ggacgtttac cttggaacta ttccgtacat gactcccaaa
     3241 ggatcatttg tgatcaatgg agctgagcga gtaatcgtat cacagcttca ccgttcacct
     3301 ggtgtattct tcggacaaag ccgccatgct aacggaacca aactttattc cgcgcggatt
     3361 attccgttca agggatcgtg gattgagttt gctacggaca ttaacaatgt gatgtatgcg
     3421 tacatcgacc gtaagaaaaa attaccggta acaacgcttt tccgtgccat tgggtaccaa
     3481 agcgatcgtg atattctcga aatctttaat cttgccgatg agatcaaggt ttccaaaaaa
     3541 gcactcaagg aagttgtcgg acgtcgtctt gctgcgaggg tactccgtac ctggatcgag
     3601 gacttcgttg atgaagatac aggtgaagtt gtttccattg agcgtaacga agtgttgatt
     3661 gatcgtgaag taattctcga agaccagcac atcgacctga ttgttgattc aggagttaaa
     3721 tccatcattc tccacagaga agaagttaac tcaggtgatt tctcgatcat ttacaataca
     3781 cttcagaaag atacttccaa cagcgaaaaa gaagctgtgg aatacatcta ccgtttgctg
     3841 cgtaacgcag aaccaccgga cgaggaaact gcacgttcgg taatcgacaa actcttcttc
     3901 tcggagcagc gttatgacct tggagaagta ggacgttacc gcatcaacaa aaaactgggt
     3961 ctgaatatgg atccgggtac acgcgtactt accaaggtag acatcattgc cattgtgaag
     4021 tacctcattg agctgattaa ctcaaaaact gatgttgatg atattgacca cctttcgaat
     4081 cgtcgtgttc gtacagtagg tgagcagctt tacaatcagt tcggtgttgg tcttgctcgt
     4141 atggcgcgta ctattcgtga gcgtatgaac gttcgcgata acgaggtgtt cactccgatt
     4201 gatttgatca atgcgaagac actttcttcg gtaatcaatt cgttcttcgg aaccaaccag
     4261 ctttctcagt tcatggacca gaccaatccg ctggcagaaa tcacgcacaa gcgtcgttta
     4321 tctgcactcg gtcccggtgg tctttcccgt gagcgcgcag gattcgaggt tcgtgacgta
     4381 cactacacgc actatggtcg tttgtgtacc atcgaaactc ctgaaggtcc gaacatcggt
     4441 ctgatctctt cactttgcgt atttgcgaaa gtgaaccgct taggatttat tgaaacacct
     4501 tatcgcaggg ttgtagaagg caaagttctc cttggagaag atccgattta ccttagcgct
     4561 gaggaagaag ataacagggt aattgcccag gcaaatgctg atatcgacac tggcggaaaa
     4621 ttccagggtg ataaagtaat tgctcgtctt gaggctgact tcccggttgt agaaccgaag
     4681 gatgttgcgc ttatggacgt tggtacaaac cagattgcct ctattgcggc ttcgcttatt
     4741 ccattcctgg agcatgacga tgccaaccgt gctctgatgg gatcgaacat gcagcgtcag
     4801 gctgttccac tacttcgtcc ggaagctcca attgttggaa ccggtattga agaattggtt
     4861 gcccgcgatt cacgtgtatt gatcaatgca gaaggcgaag gcgtagttga gtatgttgat
     4921 gccaaccaaa tcaccattcg ctatgagcgc acagacgatg agcgtttcgt tagcttcgat
     4981 ggcgatacca aaacgtataa cctcaccaaa ttccaaaaga ccaaccaaag tacatccatc
     5041 aacctgaagc caatcgttaa gaagggcgac aaggtttcaa gaggtcaagt gctttgcgaa
     5101 ggttatgcta caaaagatgg tgaactcgca ttgggacgca acctgaaagt agcgttcatg
     5161 ccatggaagg gttacaactt tgaggatgcg attgtaattt ctgagcgtgt agtgcgtgaa
     5221 gatattttca cttcgattca cattgatgaa tacattcttg aagttcgtga aacaaaacgc
     5281 ggtcttgaag aacttactgc tgatatacca aacgtatctg aagaagcaac ccgtgatcta
     5341 gatgaaagcg gaatgatccg aatcggtgct gaggttaaag aaggcgatat tcttattggt
     5401 aaaatcacac caaaaggaga aacagatcct tcacctgaag aaaaacttct tcgcgcaatc
     5461 tttggtgaca aagccggaga tgtaaaagat gcatcacttc gtgtgggtcc ctctgtaaaa
     5521 ggtgttgtta tcgataagaa attattctcg cgcgctatca aagatcgcaa gatgaaggcg
     5581 caggagaaac ctatcgttga tcagctagat aaggagtacg aaaaagaagt tgctgacctt
     5641 agaaatgttc tggttgagaa attacttcaa atcgttggcg aagagaaatc tgccggtgta
     5701 ttcaataatt tcaaagagga gatcatcaaa aaaggtgtga agttttcttc aaaggcactt
     5761 tttgcaattg attattctac tatcaatcct accaactgga cttcagttga gaagatgaac
     5821 aagatgatca atcgtttgat ccataactat aacattaagg cgaacgattt gcttggtatt
     5881 tacaagcgta aaaagttcca gcttactgtg ggtgatgaat tacctaccgg gattgtgaaa
     5941 cttgccaaag tttatcttgc tcagaagcgt aagcttcgtg taggagataa aatggcaggt
     6001 cgtcacggta acaagggtgt tgttgcgaag attgttcgtg aagaagatat gcctttcctt
     6061 gccgacggaa ctcctgttga catcgttctt aacccactgg gtgtaccatc gcgtatgaac
     6121 ctcggacaga tctatgaaac agttcttggt tgggcaggat tgaaactcgg aatgaagttc
     6181 gctacgccga tttttgacgg agcgcacatt gatgagatca acaagcttac cgatgaggct
     6241 ggtgtaccgc gttacggaag aagctatctg cacgatggtg gaaccggagt acgattcgac
     6301 cagcctgcta cagtaggagt gatctacatg atcaaactga gtcacatggt tgacgacaag
     6361 atgcacgcac gttcgattgg tccctactca ctcattaccc agcagccgct tggtggtaag
     6421 gcgcagtttg gtggacagcg ttttggtgaa atggaagtat gggcactcga agcattcggt
     6481 gctgccaata ttctccagga gatcttaacg gttaaatcgg atgacgttgt tggtcgtgcc
     6541 aaggcgtacg aatcgattgt gaaaggtgaa aaccttccgc agccgggtat ccctgaatcg
     6601 ttcaacgtac tgatgcacga attacgtggt ctcggtctca acatcacatt ggaataatcc
     6661 tcactaacgt tcaataattg aaacatggct ttcagaaaag aaaataaagt caagagcgac
     6721 ttcgataaga tcgtcatctc cctcgcttct cccgagatga tactcgaacg ctccagcggt
     6781 gaagtgctga agccggaaac gattaactat cgtacttaca aacccgaacg tgacggtttg
     6841 ttttgcgaga gaattttcgg tccggttaag gattacgaat gccactgcgg aaaatacaag
     6901 cgtattcgct ataagggaat cgtttgtgac cgttgcggag ttgaagtaac ggagaagaaa
     6961 gtacgtcgtg agcgtatggg gcacatttca cttgtggttc ctgttgcaca catctggtat
     7021 ttccgttcac ttccaaacaa gatcggttac cttcttggat taccttcgaa gaaacttgat
     7081 caggtaattt actatgagcg ttacgttgta gttcaggccg gtattgcaac tgcagccgac
     7141 ggtgcacaac ttaacatgat ggatttcctt acagaagaag aatacctcga tattcttgat
     7201 aaacttccga aggaaaacca atacctcgat gataacgatc ctaacaagtt catagcccgt
     7261 atgggagctg atgccttgca ggaattactt cgtcgtatca acctcgaaga gctatcctac
     7321 gagcttcgtc acaaggccaa cactgaaaca tcacagcaac gtaagagcga agcgctcaag
     7381 cgtctacagg tagtgcaggc tttccgcgat tccaatgagc gtatcgtgaa taagccggaa
     7441 tggatggtga tcaaagttgt tcctgtaatt ccacctgaac ttcgcccact cgttcccctg
     7501 gatggtggtc gttttgctac ttccgatttg aacgatcttt accgtcgcgt aattatccgt
     7561 aacaatcgtc tgaagcgtct gatcgagatc aaggctcctg aagtgattct ccgtaacgaa
     7621 aagcgtatgc ttcaggaagc ggtggactct cttttcgata actctcgtaa atcttctgcg
     7681 gttaaaaccg aatcgaaccg tccgcttaaa tcactttctg attcattgaa aggtaagcaa
     7741 ggtcgtttcc gtcagaacct gctcggtaag cgtgtagact attctgcacg ttcggtaatc
     7801 gtcgttggtc ctgaattgaa acttcacgag tgtggtcttc caa
//
LOCUS       DAOMYG010000097         7791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3110_length_7791_cov_8.478025, whole genome shotgun sequence.
ACCESSION   DAOMYG010000097 DAOMYG010000000
VERSION     DAOMYG010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7791
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3110_length_7791_cov_8.478025"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..1038)
                     /locus_tag="PK610_04500"
     CDS             complement(343..1038)
                     /locus_tag="PK610_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ35221.1"
                     /translation="MSNHILLVEDEESLLEVVKLNLEMEHYSVHAVMNGKEALDAALK
                     NNYDLILLDVMLPGMDGFEVCRKLRQSGLLTPVLFLTAKGTTEDRITGLKLGADDYLS
                     KPFHLEELLLRVKILLNRNKGQQEKQSEEAFFEFGNFRIDYSTYEIFEKGKRKAELGK
                     REIQLLRLLLTQPGKVVSREEILDKIWGEDAFPTSRTIDNYILVFRKHFEKNPREPRY
                     FHSVRGVGYKFTP"
     gene            complement(1067..1957)
                     /locus_tag="PK610_04505"
     CDS             complement(1067..1957)
                     /locus_tag="PK610_04505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ35222.1"
                     /translation="MLSVLIPVYNFDVRPLVEDLSTQAQRSGKAVEIICLDDGSKAEF
                     KNQNRSLSQLPGVSYEELPHNTGRSIIRNLLAKKASYDHLLFIDCDSQTESTSYIWNY
                     INALKKDAVIYGGRSYAPDAPQDHAYFLRWEYGRQREVLSPEKRKQHPYRSFMTNNFV
                     VPKSLFLSIGMDESLKGYGHEDTLFAQELRERKIEVLHIDNPLRHIGLETNKVFLEQT
                     ANGVRNLHRLIQRNKVLPDNRLFSMYKKLRGTIFGTIFYSWFRRNKQKIESNLCGLTP
                     SLRKFDLYKLGLLLEMDRSS"
     gene            complement(1959..2669)
                     /locus_tag="PK610_04510"
     CDS             complement(1959..2669)
                     /locus_tag="PK610_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ35223.1"
                     /translation="MSTVISIQNARIFQNEKLLLDEVSLDLSPGDFVYLIGRTGSGKS
                     SFLKTLYGELPLTEGQGTVGGFDLKKLKRRQIPFLRRKIGIVFQDFQLLTDRTVNDNL
                     LFVMKATGWKDKKKMNFSCEQLLQMVGLEGQGQKFPHQLSGGEQQRVSIARALVNEPE
                     IILADEPTGNLDPETSAEILNLMFSIAKNGTAVLMATHDFTIMERFPMRTIKCENGKL
                     SEVSPAAHFNPLNPLSRG"
     gene            2825..5992
                     /locus_tag="PK610_04515"
     CDS             2825..5992
                     /locus_tag="PK610_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ35224.1"
                     /translation="MKTHTPSGAKTPVAMAQTVAWMRGVVMLLLFVQPLYAQKTKIYT
                     DKYSVYREALDLYDKEKYSVARQKFDEAIKTLNDPLDEVSINAEYYAALCALKLFHPD
                     AEYLLERFVATHPESPQVRTVYMHLGRHFFQNKKWKKSIEYFDKVDRFHLTEKETHEF
                     HYKRGYAQFQLKDYKAAAVSFNEIRNKENDYQEPATYYYSHIAYFEGNYQVALDGFEK
                     LKSSENFKAMVPYYIAQIYYLQKRYDDVVAYVPPILDTAKGKQKNELSHLVGDSYYKL
                     KKYDIAVPYLEQFIASVTPTREENYQMGYAYYRSGFYDKAVPFLNKAVNGKDEMAQYA
                     NYHLADCYLRLNKKEYARNAYEGAAKLDIDRKVQEDAMFSYAKLAYELSNNPFHEAIE
                     ALQSYLKKYPEGPRRDEAYSFLINVYLTTRNYQAALDAIAQIKTKDFRVQTAYQMATF
                     NLGVDLFQKSRYVDAIKAFDNVKVYPLDKRLNAESKYWIAECKYKMKNYDEAIEAYSV
                     FQQEPGAFSSKLYNVANYNVAYAYYDKGMDELERKQNSKDNASLTSSLYAFKKFIAEP
                     QEKDKVKLADASLRAGDLHYYRKENENAIEYYNKAYDYNSGSRDYALYQIAHCRHLTR
                     KYDDAVATYNRLIRDYPSSNFVPDATFEIGEVYRENQSYQKSIDTYKQFISSYPSNQK
                     VRKATGYIALQFYQLKNYKESERYYRMLLESPNQNDIQTALDGLKNVLIALDREAEWV
                     ALVKQYDKRGESTKEIETTLWESLDRSYSANDCEKSIEKANGYLQQFPNGAYVMQARF
                     YRAECLHSKGQRELSVSDYEYVFGIAGNPFMEITSLRLGNFYFQAKNYEKSTIYYTQL
                     EKVASGATNRLNAQIGLMRGNFLVKQFGQSQDYAARVLKDATVTGDLKSEAHFIQGLS
                     YLELNDLSNAKTELQKSIDITKTGWRWAEARFNLCLITYKQGDYKKCETEIMNFVKVK
                     PNFDYWLGRSYILLADVYLGLNDPFQAKSTLRSVIDHYKGDDEIVPTAQKKYDEILER
                     ENQQNNSRKMSTGESENTEEEGGNE"
     gene            5992..7728
                     /locus_tag="PK610_04520"
     CDS             5992..7728
                     /locus_tag="PK610_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35225.1"
                     /translation="MKKLALLLVCSLSFYGTNELFAQESGGNGNYMTVIEVGLPRSLV
                     RTNRLSENPRPADTVIAVPPMTYSNVPSQAKTEFTPDKLTAANVFPKQALPKLYRFYF
                     RGGVGSYASPLAELYVNDIYNKKGSWGAHLKHFSSNSGIDDLGFSGFSDNMANVYGKL
                     YVNKLEIGGDFSYKRNVVHYYGFNPDSFPEYKERDTIRQRFQYFGANAYIATFKPDSN
                     KINHRENIGFYHFSDRYGANENFFTVTSAMNKRMGKEVYGLDMNIRYNGFLPTSNSYG
                     CITCLSEPQTATQQDNFIGELNPHIVSKGENWIAKAGLKVSMDVYSGTSYFYFYPDAE
                     FRYSLFNDIFVPYAGIDGGIQRNSYKTLADENPFLMTFQQDLRNTVRKINVYGGIRGT
                     WSSTLSFNAKVGYTALKDQVMFVTDTTFSPQTRFGMVYDDGGLLTMSGQLSYQQREKI
                     KVILRGEYFRYKLENEAYAWNIPDLKVTLSGIYDLYDKIIVRADVFFVGQRYGRSLVV
                     VDGGEQLEGQNIWAYRLKPFVDANLGVEYRYTKRISAFLNVNNIAASRYQYWYKYPVQ
                     SINVMGGFTVSF"
BASE COUNT         2265 a         1572 c         1785 g         2169 t
ORIGIN      
        1 cctgttcgaa tttcatgagt ttaatgggca aaaagatcta tggaattcaa aaaggatagg
       61 aaaagtgcta tgtgctaaaa agagaaaacc tgaaaagagt tctttaggag gaggagtgaa
      121 ctcttttcag gtaatcttgc aaggaaaata aaaccagtag aatcgtggag gtagcaaatg
      181 agccagattc taactataag acgaatatct gatttaccgg ttgcctgagt ccataaaaat
      241 tatgcgaacg gtcggattat tcgggggaac ggcaaaaaat cggggataaa tggaataagg
      301 gcaaattaac ccactgaaaa ccagttaaat ggatttagtc gctcagggtg tgaatttgta
      361 tccaacccct cgaacagaat gaaaataacg gggttcgcgc gggtttttct cgaaatgttt
      421 tcggaaaacc aggatgtaat tatcgatggt gcgcgatgtc gggaaggcgt cttcgcccca
      481 gattttatcc agaatttcct cgcgtgaaac gactttcccg ggttgggtaa ggagtaggcg
      541 gagcaattga atttcgcgct tgcccaattc ggcttttcgt tttccctttt caaaaatttc
      601 gtaagtgcta tagtcgatcc ggaaattccc gaattcaaaa aatgcttctt cggattgttt
      661 ttcctgctga cctttatttc tgttcagcag gatttttaca cgcagtaata attcttccag
      721 gtgaaaaggt ttactcaggt aatcgtcggc gccgagtttt aaaccagtaa tacgatcttc
      781 tgttgtgcct ttggcagtta gaaacaatac tggtgtaagc aaaccgcttt gacgtagttt
      841 tctgcatacc tcaaaaccat ccattccggg aagcatgaca tccagcaata tcaggtcgta
      901 attgtttttt aaagctgcat ccagcgcttc ttttccgttc atcactgcat gaaccgaata
      961 gtgttccatt tcgaggttga gtttaacaac ttcgagcaga ctttcttcat cttcaacaag
     1021 cagaatgtga ttgctcataa cggcaatatc gtaaaaattc aattaatcag ctgctgcgat
     1081 ccatctcgag caggagtcca agtttataca aatcgaattt tcgcagtgag ggagtcaatc
     1141 cgcagaggtt ggattcaatt ttctgtttgt tgcgacggaa ccagctgtaa aaaatagttc
     1201 caaaaatagt tccccggagc tttttgtaca tagaaaaaag gcggttatcg ggcaatacct
     1261 tattgcgttg gatgagccga tgtaaatttc ttacgccatt agctgtttgt tccagaaaca
     1321 ctttgttcgt ttccagtccg atatgccgca acggattatc aatatgcaat acttcaatct
     1381 tacgctcgcg caattcctgt gcaaagaggg tgtcttcatg gccataaccc ttcagcgatt
     1441 catccattcc gatggagaga aacagtgact ttggaacaac aaaattattg gtcatgaaac
     1501 tccggtaagg atgttgtttc cgtttttccg gagagagcac ttcgcgctga cggccatatt
     1561 cccaacgaag aaaataggca tgatcttgcg gtgcatccgg tgcataggat cgtccaccgt
     1621 atataacagc atctttttta agcgcattga tatagttcca gatgtacgat gttgactctg
     1681 tttgtgaatc gcaatcgata aacaagagat gatcgtagct tgcttttttt gcaaggagat
     1741 tcctgatgat gcttcttcct gtattgtggg gtaattcttc gtagctaaca ccgggaagct
     1801 gactgagtga tctgttctga tttttaaatt ccgctttgga accgtcgtcg agacaaataa
     1861 tctccactgc tttgccgctg cgttgcgcct gtgtgctgag gtcttccacc aggggtctga
     1921 catcaaaatt atagacgggt ataagaacgg agagcatatc agcccctgga taaaggattg
     1981 agcggattaa aatgcgcagc gggactaact tcactcagtt ttccgttttc gcatttgatg
     2041 gtacgcatgg ggaaccgttc cataatggtg aaatcgtgcg tagccatcag tacggctgtc
     2101 ccgttttttg caatggaaaa catgagattg agaatctccg cagaagtttc cggatcaagg
     2161 ttgcccgtag gttcatcggc aagaatgatc tcgggttcat tcaccaatgc ccgcgcaatg
     2221 gaaacccttt gttgttcacc accggagagc tgatgaggaa atttttgtcc ctgaccttcc
     2281 agtcccacca tctgtaacaa ctgctcacag gaaaaattca ttttcttctt gtctttccat
     2341 ccggttgctt tcattacaaa cagcaaattg tcgttaacag ttctatcggt aagcaattgg
     2401 aaatcctgaa acacaatacc gatttttcga cgcagaaagg gtatttgacg gcgtttcagt
     2461 ttcttcaggt cgaaccctcc aacagtaccc tgaccttcag tcaatggcag ttcgccgtaa
     2521 agggttttga gaaagctcga tttaccgctt cctgttcgtc cgatcaggta aacgaaatca
     2581 cctggagaca gatcaagcga tacctcatcg aggagaagtt tctcattctg aaaaatgcgg
     2641 gcgttctgga tgctgattac ggtcgacatg aaactattgg gattcggttg cgtaaagatg
     2701 gcaataaaag cgctgcaagc cttgcagaag cgacaagtta catgcacttt atgttaacag
     2761 aaatgggttg aaaactatta cgctttattg aggatggcct tcttggtcgg actaatttta
     2821 gtaaatgaaa acccatactc cttcgggagc aaaaacccct gttgccatgg cgcaaactgt
     2881 tgcatggatg cgtggagttg tgatgctgtt actttttgtt cagccactct acgcacagaa
     2941 aacgaaaatt tataccgaca agtattcggt ataccgtgag gcacttgatc tttacgataa
     3001 ggaaaaatat tctgttgccc gtcagaaatt cgatgaagcg atcaaaacac ttaacgatcc
     3061 tttggatgaa gtttccatca atgctgaata ttatgctgca ttgtgcgcgc tgaagttgtt
     3121 tcatccggat gcagagtatc tgctggaacg ctttgtggct acgcatcccg aatctccaca
     3181 ggtgcggaca gtttatatgc atttgggacg tcattttttt cagaataaga aatggaaaaa
     3241 gtcgattgaa tatttcgaca aggtcgaccg ttttcatctt acggaaaaag aaacccatga
     3301 attccactac aaacgtggtt atgcccaatt tcagttgaag gattataaag cggctgctgt
     3361 ttcgtttaat gagattcgca acaaggaaaa tgattaccag gaaccggcaa cgtattatta
     3421 ttcgcatatc gcctatttcg agggtaatta tcaggtagca cttgacggat ttgagaaatt
     3481 gaagtcgagc gaaaacttca aggcaatggt tccctactat attgcgcaga tctattacct
     3541 gcaaaaacgt tatgatgatg tagttgccta tgtaccgcca attcttgaca ccgccaaagg
     3601 caaacaaaaa aacgagttga gtcatttggt aggcgattcc tattacaaac taaaaaaata
     3661 cgatatcgca gttccttatc tggaacaatt tattgcatcg gtaactccta cacgcgaaga
     3721 gaattaccag atgggttacg cctattacag gagcggtttt tacgataagg cagtcccttt
     3781 tctgaataag gctgtaaacg gaaaagatga aatggcacaa tacgccaatt atcatttggc
     3841 cgattgttat ttacgcctga acaagaaaga atatgcacgc aatgcctacg aaggagcagc
     3901 gaaactggat atcgaccgca aagtgcagga agatgcgatg ttctcgtatg cgaaactggc
     3961 ctatgagtta tccaacaatc catttcacga agccattgaa gcattgcaat cgtacctgaa
     4021 gaaatacccg gaaggtccgc gtcgcgatga ggcgtattct tttctgatta atgtgtacct
     4081 cacaacgcgt aactatcagg ctgcacttga tgccattgcg cagatcaaaa caaaagattt
     4141 tagagtgcaa actgcctatc agatggccac ctttaatctg ggtgtggatc tttttcagaa
     4201 gtcgagatat gtagatgcaa ttaaagcgtt tgataacgtt aaagtatatc cgctcgacaa
     4261 acgactgaat gcagaaagca aatactggat agcggagtgc aagtacaaaa tgaaaaatta
     4321 cgatgaggcc atcgaagctt attcagtttt tcagcaggag cccggtgcgt tttcttcgaa
     4381 attatataat gttgccaact acaatgttgc ctatgcctac tacgacaaag gtatggatga
     4441 gctggagcgt aaacagaatt cgaaagacaa cgcatcgctg acctcaagtc tttatgcgtt
     4501 taaaaagttc atcgctgagc cccaggaaaa agataaagtt aaactcgccg atgcaagtct
     4561 gcgtgccggt gatttacatt attaccgtaa ggaaaatgaa aatgccattg agtattacaa
     4621 caaggcttac gattacaatt ccggatcacg cgattacgcc ttgtatcaga ttgcgcactg
     4681 tcgtcacctg acccgcaaat acgacgatgc agttgcaact tacaaccgat tgatacgcga
     4741 ttacccttca agtaattttg tgcccgatgc tacttttgaa attggtgaag tgtaccgcga
     4801 aaatcaaagt tatcagaaga gtattgatac ctacaagcaa tttatttcga gttatccgag
     4861 caatcaaaaa gtacgaaaag caacagggta cattgcactg cagttttatc aattgaaaaa
     4921 ctacaaggaa tcggagcggt attaccgaat gcttctggag agtccaaacc agaatgatat
     4981 ccagactgca ctcgacgggt tgaagaatgt tctgattgcg ctcgacaggg aggccgaatg
     5041 ggttgcattg gtgaagcagt acgataaacg cggggagtca accaaagaaa ttgaaaccac
     5101 attatgggaa tcgcttgatc gctcgtattc ggcaaacgat tgcgaaaagt cgatcgaaaa
     5161 agcgaacggt tacctgcagc agttcccgaa tggagcttac gtgatgcagg cgcgattcta
     5221 cagagctgaa tgccttcact ccaaagggca acgcgaattg tctgtttctg attacgaata
     5281 tgtattcggc atcgcaggaa atccctttat ggagatcacc agtttgcgtc ttggtaattt
     5341 ttatttccag gccaagaatt acgagaagag cacgatttat tatacccagc ttgaaaaagt
     5401 cgcttcagga gctaccaatc gtttgaatgc acaaatcgga ttgatgcgtg gaaattttct
     5461 ggtgaaacaa tttggacagt cgcaggatta tgcagcacgg gtattgaaag acgccacggt
     5521 aaccggtgat ttgaaatcgg aagcgcattt tattcaggga ctcagttacc tggaattaaa
     5581 cgatctcagc aatgcgaaga cggaactgca aaaatcaatc gacattacca aaacaggatg
     5641 gcgctgggca gaggcacgct ttaatctttg tttgatcact tacaaacaag gcgattacaa
     5701 aaagtgcgaa acagaaatca tgaattttgt gaaggtgaaa ccgaattttg attactggct
     5761 tggacgatcg tacattcttc tggcagatgt ctatctcgga ctgaatgatc ccttccaggc
     5821 gaaatctacc ttgcgaagtg tgattgatca ttacaaaggg gatgatgaaa ttgttcctac
     5881 agcgcagaag aaatacgatg aaattctcga gcgcgaaaat caacagaata attccaggaa
     5941 aatgtcgacc ggtgagtcgg aaaataccga agaagaagga ggaaatgaat aatgaaaaag
     6001 ttagctcttt tactcgtttg ttcgttgtcg ttttacggca caaacgaact tttcgcgcag
     6061 gaaagcggtg ggaatggcaa ttacatgacc gtaattgaag tcggcctgcc ccgctcgctg
     6121 gttcgcacca atcgcctttc ggaaaatccg cgtcctgccg atacggttat cgctgttcct
     6181 ccaatgacct atagcaatgt cccctcacag gctaaaaccg aattcactcc ggataaactc
     6241 actgcagcca atgtttttcc taaacaggcg ctgccaaagt tgtaccgttt ctatttccgg
     6301 ggcggtgttg ggagttatgc ttcaccactt gcagaattgt atgtaaacga catttacaac
     6361 aagaaaggaa gctggggcgc gcatttgaaa catttttcct ccaattccgg aatcgacgat
     6421 ttaggattca gcggtttttc ggataacatg gctaacgtat atggtaaatt gtatgtaaat
     6481 aagttagaga taggcggtga tttttcgtac aagcgaaatg tggtgcatta ttacggattc
     6541 aatcccgata gttttccgga gtacaaagaa cgcgatacca ttcgtcagcg ttttcagtat
     6601 tttggggcga atgcctatat cgcaacattc aagcccgatt ccaacaagat taaccacagg
     6661 gagaatattg ggttttatca tttcagcgac aggtacggag ccaatgaaaa tttctttacc
     6721 gtaacaagtg caatgaataa acgcatgggg aaagaagtgt acggattgga tatgaatatc
     6781 cgttataatg gttttttgcc tacgagtaat tcgtacggat gcataacctg tttgtcggag
     6841 ccgcaaactg caacgcaaca ggataatttt attggcgaat tgaatccgca tattgtgagt
     6901 aaaggggaaa actggatagc aaaagcgggt ttaaaagtga gtatggatgt gtattccggt
     6961 acgagttatt tttatttcta tcctgatgct gaattcaggt acagtttgtt caatgatatt
     7021 tttgttccct atgcaggaat cgatggtggt atacagcgta acagctataa aacccttgcc
     7081 gatgaaaatc cattcctgat gacctttcag caggatttgc ggaatacggt aagaaagatc
     7141 aatgtgtacg gtggtattcg cgggacatgg agttctacct tgtcgtttaa tgcgaaagtg
     7201 ggatacaccg cgctgaagga tcaggttatg tttgttacgg acaccacatt ttctcctcag
     7261 acccgattcg gcatggtcta cgacgatgga ggattgctta ccatgagtgg gcagttgagc
     7321 tatcagcaac gcgaaaaaat aaaggtgatt cttcgtggtg aatatttccg ctacaaactc
     7381 gaaaacgaag cttatgcatg gaatatcccg gatttaaaag ttacgctttc gggtatttat
     7441 gatctctacg acaagattat tgtccgcgct gatgttttct ttgtggggca acgttacggt
     7501 cgttctttgg tggttgtaga tggcggggag cagttggaag gacagaacat ttgggcctac
     7561 cgtctgaaac cttttgttga tgcaaatctt ggggttgaat accgttatac caagcggatt
     7621 tctgcattcc tgaatgtgaa taatatcgca gcttcacgtt atcagtattg gtacaaatat
     7681 cctgtgcaat ccattaacgt aatgggtgga tttacagtgt cgttttaagc ctgtttttca
     7741 gatactaaca tttaattcgg aaaagcacct tttcaggtgc tttttcttat t
//
LOCUS       DAOMYG010000098         7791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3111_length_7791_cov_7.266029, whole genome shotgun sequence.
ACCESSION   DAOMYG010000098 DAOMYG010000000
VERSION     DAOMYG010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7791
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3111_length_7791_cov_7.266029"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..675
                     /locus_tag="PK610_04525"
     CDS             61..675
                     /locus_tag="PK610_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRJ35226.1"
                     /translation="MKIICIGKNYADHAKELNSAVPEEPVFFLKPDTAVLPKQSAFFY
                     PDFTNNLHHEIEIVLKIGKNGKHIDPKFALTYVTEIGLGIDFTARDLQEKAKQKGLPW
                     EIAKAFDNAAVVGNTFIPIASTGAIPELNFRLDINGNTVQNGKASDMVFPVETLISYV
                     SKFITLKKGDLLFTGTPAGVGPVKIGDRLQGYLMDKKLIDFKIN"
     gene            complement(678..2348)
                     /locus_tag="PK610_04530"
     CDS             complement(678..2348)
                     /locus_tag="PK610_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRJ35227.1"
                     /translation="MQSIMGWSRGLLLILLCLTIQFSKAQTGVFFVAFNDRNGSIHTI
                     DRPEEFLSERALQRRKDQGIPIRENDLPVSGVYVSVLKGMNGVKVVKTSKWLNGAVIE
                     ISDSLSFVQLQGLSFVRQIHRIDNRKEGKEKQLDPDNLRDMYLEMHADHLYGSSFNQI
                     RMLGGNLMHRMGYDGKGKVIAVLDAGFPGIYQMECFRKMQDEGRLLGTWDVVENESDV
                     TDNNSLHGTVVLSCMASYWPGIFIGTAPGAAYWLLVTENIDSETLQEEYNWVIGAEFA
                     DSVGADIINSSLGYTTFDDSLQNHSYADMDGNTTPCSIGADIAASKGILVVNSAGNMG
                     VDPWHHIVAPSDGDSVLAIGAVNENREFAFFSSHGYSADGDVKPNVAAQGWNAVVVWP
                     WNEVGLANGTSFSSPILSGMAATLWQSHPGRTNMEIFHAIEESAHLFPLYDSLMGFGI
                     PDFYRAYINLGGTPHYELDPLEILDVYPVPFSRDLYIDMNVAAQSSCIIAFYDVAGRP
                     LKEEVVQLVSGSINKLVISDPLAGFPSGVYAMRITWNNGTTRMKIVKF"
     gene            complement(2323..3000)
                     /locus_tag="PK610_04535"
     CDS             complement(2323..3000)
                     /locus_tag="PK610_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35228.1"
                     /translation="MRNKLLLFSLLLALSACRREEDPALSDLGYSYFPLEVGKYVIYS
                     VTEIDVDAPISRFDTTRYQIKEKLESVFTDNAGQPCIRVERYRRPTPANPWVINDVWY
                     FYRNERNAQKVEENERFIRLSFPINYDARWNGNAFLPADEWIYRYENIGVSYSNPYQT
                     FSKTVEVNQRFRYNFVEYENCREIYADGIGMVYKQYIVTTINNGDSTSINRGKLLHQY
                     AIDYGLE"
     gene            complement(2990..3799)
                     /locus_tag="PK610_04540"
     CDS             complement(2990..3799)
                     /locus_tag="PK610_04540"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HRJ35229.1"
                     /translation="MMKAKQSGLFLVLFFLLIGTAWSQQINQTDAKGKKQGMWDVKYP
                     NSQVSKYKGTYKDGKPQGEFIHFYESGKVKCKIKYATDGKTSRAVYFHDDSGKRLSDG
                     KYINQKRDSVWNFYDREGIKSSTEEFKNDLLHGKRTIYYLSGKVSEVIQYSNGKKNGE
                     STRYFEDGKVMMKSTYKDDIQIGEALTYHPGGKVMFKENYENGKLEGLRIVYDESGKE
                     ISRNWFSGGRMLTKEEEEKYIIELQKKNQQKTPQQKQQPKQTPNGTKQGAK"
     gene            complement(3818..4951)
                     /locus_tag="PK610_04545"
     CDS             complement(3818..4951)
                     /locus_tag="PK610_04545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ35230.1"
                     /translation="MKIAVVGPAWPLRGGIANFNEALALSLQDEGHDVRIYSFSLQYP
                     SILFPGSTQFDNGPAPQGLHIETCINSINPFSWFRSAKTIKAWKPDLVIVRFWLPFMG
                     PSLGCVSRRLRKIAPVIAITDNVIPHEHRPGDRLLTRYFVRSCDAFIAMSRAVLLDLD
                     RFDKRKPREYLPHPIYSIFGDQLEKSDALANLQLDPEFRYLLFFGIVRKYKGLDLLLE
                     AIARVDHKSLKIKLLIAGEFYEDKEEYLKLIREKGLEDVILLHDKYIPSTEVRNYFCA
                     ASLVTQTYRTATQSGVTQIAYHFSRPMLVTDVGGLAETVPHGKVGYVTSLEPSVIAAS
                     ITDYFANSREEEMARNVEEDKKRFDWSFFIKGIMTLKDKLNRS"
     gene            complement(4948..5925)
                     /locus_tag="PK610_04550"
     CDS             complement(4948..5925)
                     /locus_tag="PK610_04550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRJ35231.1"
                     /translation="MKIQNYPYEISIVVPLKSEEESLPELAQWIDRVCVEHNYSYELI
                     LIDDGSDDNSWEVIEQLSSSNPSVKGIRFRRNYGKSAALHVGFQAVRGRVVITMDADL
                     QDSPDEIPDLYKMITEQKFDLVSGWKKKRHDPLMKTIPTKLFNWATRKMSGIYLHDFN
                     CGLKAYRHDVVKSVEVMGEMHRYIPVIAKWNGFVKIGEKVVHHQARKYGSSKFGWNRF
                     INGFLDLFTITFVSRFSKKPMHFFGALGTLMFAIGFLLALYMGIDKLFIHPDAEKIAL
                     RPVFYIALVTMVLGTQLFLTGFLAELLVRNSPASDNYQVSERKGDLPQA"
     gene            complement(6058..6801)
                     /locus_tag="PK610_04555"
     CDS             complement(6058..6801)
                     /locus_tag="PK610_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HRJ35232.1"
                     /translation="MSASLMASDVVFFGELHNNAIAHWLQLRLTQAAFEVKGKQLLLA
                     AEMFEADNQSGMDEYLAGKIDEKQFAEKVRLWPNYATDYKPLVDFAKDNGLRFICANI
                     PRKYASLLYKKGVDSLMVISPEEKAWIAPLPFPYDANVGSYKRMIELSGGHGGENMPK
                     SQAIKDATMAHFISTNFKPGNFCIHFNGSWHSDDWEGILWYMKHYAPKLKLATITTVQ
                     QKNPLKLEKENKGRADFVLVVAEDMTNTH"
     gene            6785..7000
                     /locus_tag="PK610_04560"
     CDS             6785..7000
                     /locus_tag="PK610_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35233.1"
                     /translation="MSEALILENFTGLPSLLNNAKATGSSAKARSGFVPDNNRIVQTI
                     SVRIIMNLFSKIPILCPQGSIQCIFAE"
     gene            6997..>7791
                     /gene="mnmA"
                     /locus_tag="PK610_04565"
     CDS             6997..>7791
                     /gene="mnmA"
                     /locus_tag="PK610_04565"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009421636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRJ35234.1"
                     /translation="MKRVVVGLSGGVDSSVAAWLLQEEGYEVIGLFMRNWTDDSVILN
                     NECPWIDDSTDAMLVAEKLGIPFQTIDFSAEYKERIVDYMFSEYEKGRTPNPDVLCNR
                     EIKFDVFLEAALKLGADFVATGHYCRKTTTTANGKEIHHLLAGKDPGKDQSYFLCQVS
                     QEQLSKALFPIGHLLKSEVRKIAHEIGLATADKKDSQGLCFVGQISLPEFLQQKLDTR
                     KGKVIEIPNDISLFGEYESLPETDFIALSKPFVYSEEMGKQVGEHQG"
BASE COUNT         2271 a         1745 c         1543 g         2232 t
ORIGIN      
        1 gggtgatttt ccgctttaca cgaaaaaaat cattaccgaa ctacgaaaca attcaaaagc
       61 atgaagatca tttgtattgg gaaaaactac gccgatcatg ccaaagaact caacagtgca
      121 gtacctgaag aacctgtttt ctttttgaaa cccgacactg ctgttcttcc aaagcaaagc
      181 gcgttttttt accctgattt tacgaataac ctccaccacg aaatcgagat cgttcttaaa
      241 atcggaaaaa acggtaaaca tatcgatccg aaatttgcat taacgtatgt aacagagatc
      301 ggactcggaa ttgattttac agcgcgtgat ctgcaggaga aagcaaaaca aaaaggtttg
      361 ccctgggaaa ttgcaaaagc atttgataat gccgctgttg ttggcaatac atttattcca
      421 attgccagta caggtgccat tcctgaactg aatttcaggt tggatatcaa tggcaatacc
      481 gtgcaaaacg gtaaagcatc ggacatggtt ttcccggttg aaactctgat ttcctacgta
      541 tcaaaattca tcacactcaa aaaaggtgat ctactgttta ccggtactcc ggcgggtgtt
      601 ggtccggtga aaattggaga tcgactgcaa ggttatttga tggacaaaaa gctgatcgat
      661 tttaaaatca actgagctta aaatttcacg attttcatac gtgtagttcc attattccaa
      721 gtaatccgca tagcataaac tcccgaagga aatcctgcaa gcggatcact aatcaccagt
      781 ttattgatgc tcccgctaac aagctgaaca acctcctctt taagcggtcg accggcaaca
      841 tcgtaaaaag cgatgataca ggatgattgt gcagcaacat tcatgtcgat atataaatca
      901 cgtgaaaaag gtaccggata tacatccaga atttccagag gatccaactc ataatgagga
      961 gtgccgccta gattgatgta ggctcggtaa aaatcaggaa ttccaaatcc cattaacgaa
     1021 tcgtagagag gaaataaatg tgcactctct tcaatggcat gaaaaatttc catgttggta
     1081 cgacccggat gtgactgcca cagcgttgcc gccatgcctg aaagtatagg agaagaaaaa
     1141 gaagtaccat ttgctagtcc aacctcgttc caaggccaca ccacaacggc attccatccc
     1201 tgtgctgcaa cattgggttt tacatcacca tcagcagaat aaccatgcga agaaaaaaat
     1261 gcaaattccc tgttttcatt tactgctcca atagcaagta cactatcgcc atcggaaggt
     1321 gcaacaatgt gatgccaggg atcaaccccc atatttcctg cgctgtttac aacaagaatt
     1381 cccttagaag ctgcaatatc tgcaccaatt gaacagggtg tagtattacc gtccatatcg
     1441 gcatacgaat ggttttgcaa actatcatcg aaagtggtat agcccaatga agaattgata
     1501 atatcggcgc ctacactgtc tgcaaactca gcgccaataa cccaattgta ttcctcctgt
     1561 aaggtttcgc tatcgatatt ttccgtaacc agcaaccaat acgcagctcc cggtgcagtt
     1621 ccaataaaaa taccgggcca atacgatgcc atacaggaaa gcactaccgt tccgtgcaat
     1681 gaattgttat cggtaacgtc ggactcattt tctactacat cccaagtgcc gagtagtcgc
     1741 ccttcatcct gcatttttcg gaaacattcc atctgataaa ttccgggaaa accggcatca
     1801 agaaccgcta tcacttttcc ctttccatcg tagcccatcc gatgcatcaa atttccaccc
     1861 aacattcgaa tctgattgaa tgaacttcca taaaggtgat cagcatgcat ctcaaggtac
     1921 atgtcgcgca aattatccgg atccaattgc ttttcttttc cttctttgcg attgtcgatg
     1981 cgatgaattt gccgaacaaa cgaaagacct tgcaactgaa caaaggacaa tgaatcgctg
     2041 atttctatca ctgcaccgtt gagccatttg ctggttttga caactttaac cccgttcatt
     2101 ccctttagga cagaaacata aacaccggaa acaggaagat cattttcccg aatgggaatt
     2161 ccctgatctt tccttcgctg aagtgcacgt tccgacaaaa attcttcagg tcgatcaata
     2221 gtatgtatcg aaccgttgcg atcattgaat gctacaaaaa atactcccgt ctgcgccttt
     2281 gaaaactgaa tagtgaggca caataaaatc aaaagaagtc ctctactcca acccataatc
     2341 gattgcatac tgatgcagga gtttaccacg gttaatggat gtggagtcgc cattgttgat
     2401 tgtggtaact atgtattgct tataaaccat cccaatacca tctgcgtaga tttcgcggca
     2461 attttcatat tccacaaaat tatatcggaa acgttgattc acctcaactg ttttactgaa
     2521 tgtttggtaa ggattcgagt atgaaacccc aatgttttca tagcggtaaa tccattcatc
     2581 ggcaggaaga aatgcatttc cattccagcg tgcatcataa ttgatcggga aacttagtcg
     2641 gataaatcgc tcgttctctt ctactttctg tgcgttgcgc tcattacggt aaaaatacca
     2701 aacatcattt ataacccatg ggtttgccgg tgttggccgg cgatatcgtt caacacggat
     2761 acagggctga cctgcattat cggtaaatac cgactcgagc ttttccttga tttggtaacg
     2821 tgtggtgtcg aaccgactta taggtgcgtc tacatcaatt tcggtaacgg aatagatcac
     2881 atacttaccc acttccaaag gaaaatagga ataccccaaa tcagacaatg ccggatcttc
     2941 ttctcgtcgg caagccgata gagcgagcaa taatgagaag aggagtaatt tatttcgcac
     3001 cttgtttggt tccgtttggt gtttgtttgg gttgttgttt ttgctgtggt gttttttgct
     3061 ggttcttttt ctgtaattcg attatgtact tttcttcttc ttcttttgtg agcatccttc
     3121 ctccggagaa ccaattcctc gaaatctctt ttccactttc atcgtaaaca atccgaagtc
     3181 cctcaagttt accgttctca taattttcct taaacatcac ttttccgccg ggatggtagg
     3241 taagtgcttc tccaatctga atatcatctt tataggtcga cttcatcatc acttttccgt
     3301 cttcaaaata acgggtggat tcaccattct ttttaccgtt actgtattga attacttctg
     3361 aaacctttcc ggataaataa taaatggtcc gctttccatg cagcaggtcg tttttgaatt
     3421 cttcggttga acttttaatc ccttctctgt cgtaaaaatt ccaaacacta tctcttttct
     3481 ggttaatgta ttttccatcc gataaccttt taccggaatc atcatggaaa taaactgctc
     3541 tggaggtctt gccatccgta gcatatttga ttttgcattt aacttttccg ctttcgtaaa
     3601 agtgaatgaa ctcaccttgt ggcttgccgt ccttgtaggt gcctttgtat ttcgaaactt
     3661 gtgaattggg atatttcaca tcccacattc cctgtttctt tcctttggca tctgtctggt
     3721 tgatctgttg cgaccaggca gtccctatca aaaggaaaaa cagcactaaa aataacccgc
     3781 tttgcttcgc tttcatcatc tctcaaattt aatcaattca gcttctgttg agcttatctt
     3841 ttaaagtcat tattccctta ataaaaaacg accaatcgaa ccgcttttta tcctcttcca
     3901 cattccgtgc catctcttct tctctgctgt tggcaaaata atcggtgatg gatgctgcta
     3961 taacggaagg ctccaggcta gttacataac caaccttacc atgtggcact gtttcggcca
     4021 atcctcctac atctgtaaca agcattggcc gcgaaaaatg ataagcaatc tgagttacgc
     4081 cactttgtgt tgctgttcga taggtttggg taaccagact cgctgcacaa aaataatttc
     4141 ggacctcggt cgatggaata tatttatcat gcagaaggat cacatcttcc aaacctttct
     4201 cccggatcaa tttcaggtac tcttccttat cttcataaaa ctctccggct ataagcagtt
     4261 ttattttcaa tgatttatga tcaacacggg ctattgcttc caaaagaaga tcaagtccct
     4321 tgtacttgcg aacaatccca aaaaacaaca agtacctgaa ttccggatcc aattgaaggt
     4381 tagccagagc atcacttttt tcaagctgat ctccgaaaat cgaataaata ggatgaggaa
     4441 ggtattcgcg tggcttacgc ttatcaaatc gatcgagatc caaaagcact gcacgcgaca
     4501 tggcgataaa ggcatcgcag gaacgaacaa aataccgggt caacaatcga tcgccgggtc
     4561 tgtgttcgtg aggaattaca ttatcggtga tagcaattac cggtgcgatt tttctcaatc
     4621 ttcgggaaac acaccctaaa gatggaccca taaacggaag ccagaatctt acaattacaa
     4681 gatccggttt ccatgctttt attgtttttg ctgaacggaa ccaggaaaag ggattgatcg
     4741 aattgatgca ggtttcgata tgcaatcctt gtggggcagg accattatca aattgggtac
     4801 tccctggaaa aaggatggat ggatactgaa gggaaaaaga ataaatacga acatcatgtc
     4861 cctcatcttg tagtgagaga gccaatgcct cattaaagtt ggcaattccg cctctgagag
     4921 gccaagctgg tccaactacc gcaattttca cgcctgaggt agatctcctt ttcgttcaga
     4981 aacctggtaa ttgtcgctcg ccggagaatt ccgtaccaat aattcagcta aaaatccggt
     5041 caggaaaagc tgggtaccaa gaaccatggt aaccaatgca atatagaaca caggacgaag
     5101 agcgatcttt tcagcatccg ggtggataaa gagtttatcg atacccatat acaatgccaa
     5161 aagaaaacca atggcgaaca ttaaagtacc caatgcaccg aaaaaatgca tgggtttttt
     5221 actgaaccta ctgacgaatg ttatggtgaa taaatccaaa aatccattga tgaaacggtt
     5281 ccaaccaaac ttggatgatc cgtattttcg ggcctggtga tgcactactt tttcacctat
     5341 cttaacaaag ccattccact tggcaatcac cggaatataa cgatgcatct cacccattac
     5401 ttcaaccgat tttactacat cgtgacgata agctttaaga ccgcaattga agtcatggag
     5461 gtaaatcccc gacatctttc tggtggccca gttgaagagt tttgtgggga ttgtcttcat
     5521 gagaggatca tgccttttct ttttccagcc tgataccaga tcgaatttct gttcggtgat
     5581 cattttgtac aggtcgggaa tttcatccgg cgaatcctga agatcggcgt ccattgtaat
     5641 caccactctg ccccgaacgg cctgaaaacc gacatgcagc gctgccgatt taccataatt
     5701 acggcgaaag cgaattcctt ttactgaagg attggatgaa gaaagttgtt caatcacttc
     5761 ccaggaatta tcatcactgc catcgtcgat gaggatcaat tcataggaat agttatgctc
     5821 aacacataca cggtcaatcc attgagcaag ttccggtaag gattcttctt cgctctttaa
     5881 gggcacaaca atggaaatct cgtatgggta attctgaatt ttcataggat gcaaaaaaca
     5941 aatatttagg cttggtctga taggaaccga aaaggaacta tgagcaaagc ccggttaata
     6001 tcccgaacat aggaattatc ggcttttgat tagtattcct taggttattt taggatttta
     6061 gtgggtgttc gtcatatcct cggctaccac caacacaaaa tctgccctgc ctttgttctc
     6121 tttttccagt ttgagcggat ttttttgctg aactgtagta atagtagcca gtttaagttt
     6181 tggtgcataa tgtttcatgt accacaaaat accctcccaa tcgtcggaat gccatgatcc
     6241 gttaaaatgg atgcaaaaat taccgggttt aaaattcgtg ctgatgaaat gtgccatcgt
     6301 tgcatccttt atagcctgcg acttcggcat attttcaccc ccatgtccac cgcttaattc
     6361 aatcatccgt ttgtaactgc caacattggc atcatatgga aaaggcagtg gcgctatcca
     6421 ggctttttct tcaggactaa taaccataag ggaatcgaca ccctttttat acaacagact
     6481 ggcatacttt cggggaatat ttgcacagat aaaacgtaga ccattatcct ttgcaaaatc
     6541 taccagaggc ttataatcag ttgcataatt gggccaaagc cggacctttt ctgcaaattg
     6601 cttttcatca attttcccgg caaggtattc gtccataccc gattggttat cggcttcaaa
     6661 catttcggca gccaataaca gttgctttcc cttaacttca aatgcagctt gagtgagtcg
     6721 cagctgaagc cagtgtgcga tggcattatt atggagttct ccaaaaaaga ccacatcgct
     6781 cgccatgagc gaagcactca ttttagaaaa ttttacgggt ttgccttctt tattgaacaa
     6841 tgcaaaagct actggctcct ctgcaaaagc cagaagtggg tttgttccgg ataataatag
     6901 aattgtgcaa acaataagtg tacggatcat catgaatctt ttctcaaaaa taccaattct
     6961 ctgccctcaa ggttcaatac aatgtatctt tgctgaatga aacgcgttgt tgtcggacta
     7021 tcaggtggtg tagactcaag tgttgcggcc tggttgctgc aagaggaggg ttatgaagtg
     7081 attggtctgt ttatgcgaaa ctggacagac gattctgtta ttcttaataa tgaatgtccc
     7141 tggatcgacg acagcacaga tgctatgctg gtggcagaaa aattgggaat accgtttcaa
     7201 accatcgatt tttccgcaga gtacaaagag cgtatcgttg attatatgtt cagcgaatac
     7261 gaaaaaggac gaacacccaa tcctgatgtt ttatgcaaca gagagatcaa attcgacgtt
     7321 tttctggagg ccgcattaaa actgggtgcc gattttgttg caacaggaca ttactgccgt
     7381 aaaacaacaa caactgctaa cggaaaggaa atccatcatt tgctggcagg aaaagatccc
     7441 ggtaaagacc agagttattt cctgtgtcag gtatcgcagg aacaattaag taaagcactt
     7501 tttccaattg gacacctgct caaaagtgag gtcagaaaga ttgcccatga gattggtttg
     7561 gcaactgcag acaaaaaaga ttcgcaggga ctgtgtttcg ttggtcagat cagtctgccc
     7621 gaattcctgc agcaaaaact ggatacccgc aaaggaaaag tcattgaaat tcccaacgac
     7681 atttctctgt tcggcgaata cgaatcacta cctgaaacgg attttattgc attaagcaaa
     7741 ccctttgttt attcagaaga aatgggaaaa caggtgggtg aacatcaggg a
//
LOCUS       DAOMYG010000099         7779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3121_length_7779_cov_8.856939, whole genome shotgun sequence.
ACCESSION   DAOMYG010000099 DAOMYG010000000
VERSION     DAOMYG010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3121_length_7779_cov_8.856939"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="PK610_04570"
     CDS             complement(<1..921)
                     /locus_tag="PK610_04570"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HRJ35235.1"
                     /translation="MFAILGIVLLGTVGLCALEGYSIIDAFYMTLITISTVGFGEVEP
                     LSPEGRLFVSGMIVTSFVIIGVAAASISSYVLDGYYREDLFKQQKRKILAAMKDHVIV
                     CGYGRVGSQTVERLLKGNHKVVVIEKNISGIIFERRDDLLIIQGDATQDETLAEAGIE
                     KAHTLIAALPSDADNLFVVVSAHTINPSLNIVSRASDRSSMKKIRNAGANHVIMPDTL
                     GGEYLAQLVVQPDLMEFISMISQQADDMVNLEEVGFNSLPDGFHYKTIGQLNSDSLTG
                     CTIIGFRKPSGEIEVNPSPQTEVIPNSKLFV"
     gene            complement(966..1193)
                     /locus_tag="PK610_04575"
     CDS             complement(966..1193)
                     /locus_tag="PK610_04575"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRJ35236.1"
                     /translation="MINKIQAYFERQAFGVCNWWGHKLGIDTLRVRKSFIYLSFITVG
                     SPLVLYLIMAFILENKRYFKPGSRKRSIWDI"
     gene            complement(1183..2466)
                     /locus_tag="PK610_04580"
     CDS             complement(1183..2466)
                     /locus_tag="PK610_04580"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HRJ35237.1"
                     /translation="MTEDYLHHVWKYRLFTSGDLKTTSGETIFILHPGQHNSDSGPDF
                     FDARIRIGSTLWAGNVEIHVRASEWRKHRHQFDEAYDNVILHVVFQHDEEVMNSRGRQ
                     LLCMELSNRIDPEGYERYKAFIWGRRDFSCTGQFSELPEVLIRSQFDRALAERLERKI
                     IPIRRDIAECNNNWNEVIYRALARNFGFRVNTLPFEWLARNTPHHFVRKVSGDRMYME
                     SLLFGQAGFLDQEDADEYQRNLTIRYDLVQQRLQLPVFERPVWKFSRMRPVNFPTIRL
                     AQFAILMHEETNLEAMLLRSTGAEEWSERLRLTPSDYWMRHYRFGKLSRLNPKRMGLD
                     SSQNIIINSVVPLLFCYGRDKGVDRYSESALELLEEVMPEKNRITTRFRHAGRKALNA
                     MDTQSQIEMWNMYCGPKKCLNCTIGKQLLENRYDQ"
     gene            complement(2540..3364)
                     /gene="pyrF"
                     /locus_tag="PK610_04585"
     CDS             complement(2540..3364)
                     /gene="pyrF"
                     /locus_tag="PK610_04585"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRJ35238.1"
                     /translation="MNRNELITQIRRKGSFLCVGLDTDPQKLPALFAKDEDAVFNFNK
                     AIIDATHDLCVAYKPNTAFYESLGVSGWIALEKTIDYLNTKYPDHFTIADAKRGDIGN
                     TSAMYAKAFLDKMNFDSVTVAPYMGEDSVTPFLGKEGKWVILLALTSNKGALDLQTLN
                     VGPADQVEEKLFERVLKVSGQWGNASNMMYVVGATQASMLERIRQIVPDHFLLVPGVG
                     AQGGSLEDVARFGMNKDCGLLVNASRSILYASSGSDFAEKAREEALNMQQQMAALL"
     gene            complement(3366..4439)
                     /gene="prfA"
                     /locus_tag="PK610_04590"
     CDS             complement(3366..4439)
                     /gene="prfA"
                     /locus_tag="PK610_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRJ35239.1"
                     /translation="MRSKLLAIADRYREVSELITDPAVIADMKRYVRLNKEYKDLGPI
                     VSASDEYEKTIEGIDGTLDLLRTEKDEAFREMAKAELDSLQQRKAELEDEIKVLLIPK
                     DPEDSKNAVVEIRSGTGGDEAAIFAGDLYRMYTRYFAKKGWQVEVMSVSESEVGGYKE
                     VSLLVSGEEVYGFLKFESGVHRVQRVPETEAQGRVHTSAATVAVLPEADEVDVEINMN
                     DIKKDVFRASGAGGQHVNRTESAVRLTHIPSGLVVECQDERSQIKNVERALKVLRSRL
                     YDRELSKALEERAQKRKTMVSTGDRSAKIRTYNYPQGRVTDHRIGLTLYNLAAIMEGD
                     IEEIIDALRMAENAEKLKSGLEE"
     gene            complement(4466..5647)
                     /locus_tag="PK610_04595"
     CDS             complement(4466..5647)
                     /locus_tag="PK610_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRJ35240.1"
                     /translation="MSDNRYNQRGVSASKEDVHNAIANLDKGLFPKAFCKIVPDLLTG
                     SEEHCVVMHADGAGTKSSLAYAYWKETGDLSVWKGIAQDAIVMNLDDLLCVGAVDNIL
                     LSSTIGRNKHLIPGEVISAIINGTEELLEELRSYGIGIWSTGGETADVGDLVRTIIVD
                     STVTCRMRRDAVVSNHTIAPGNVIVGLSSFGKANYEKEYNGGMGSNGLTSARHDVFNK
                     SIAKKFPQTYDPAVPEDLAYSGGLNLTDEIIVDENTREKMTAGKLVLSPTRTYAPIIK
                     AMLTEYRHHIFGMVHCSGGAQTKVLHFVDDVHVIKDNLFPVPPLFKMIHEQSGTEWSE
                     MYKVFNMGHRMEIYTDEKTADGLIALSRSFGVDARVVGRVEDSEKKKLTIRSPFGEFI
                     Y"
     gene            complement(5711..>7779)
                     /locus_tag="PK610_04600"
     CDS             complement(5711..>7779)
                     /locus_tag="PK610_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ35241.1"
                     /translation="LVLTLSASAQKNHGKEAEAAYLNEQFFTAKDLYKVAAEKEKKPE
                     KKMFYYFRVGECYRFLTDVDKAEEFYQRCVDGNYKDAIVYLRLADAQREQGKYKEAEK
                     NYTTYFQKSGDVQGKNGAEACKLAQEWKKTPTRHIIENELILNTEYYDFTPMFMDKKN
                     TTLVFSSSRPGGVGTIEEKRTGGGRTDLWVTERDKKGKWGEPKILPQGINTDDNEGAA
                     VLDRKGDVMYYTNCPNEKKKNLGCDILVVERKGDKWGTPTKIPSLKPHDSISVGHPAL
                     TGDDMALIFASDMPGGEGGKDLWMVTYDKRAKSWSDPVNLGPKINTPGDEMFPFIHSD
                     GSLYFSSDGHLGMGGLDMFRAEAVGTEKKWENPKNLGYPMNSPQHDFGIVFEGKDKGF
                     FTSNRVGGKGRDDLYSFVMPPLLYELKIFVLDEETKQPIKDIEVKLAGTDNTMFSLKT
                     NDAGSVSFITAAEGKRYVNEATNYTVEATLPKGFLKSMELKKQFTTVGVDKSTNWEFT
                     FYMKKYVGKPLRLPMIVYAYDKADLIVDLEGTKKENETKRPVNSQDSLLYLYNMMVEN
                     PTIKVQLRSHTDFRGNDAYNEKLSQRRAQTCVDFLVSKGIPKERLVAKGMGEKEPTYK
                     VVGRDTVWLSEPFIKKMTSVEEQNAAHQMNRRTDFKILNFDYVPTEADLKLQWIDLPW
                     MKRRED"
BASE COUNT         2195 a         1824 c         1413 g         2347 t
ORIGIN      
        1 cacaaacaac tttgaatttg gaataacttc tgtttgcgga gaggggttaa cctctatttc
       61 gcctgaaggt tttctgaaac caataattgt acaacctgtt agagaatcgg aattcaactg
      121 accaattgtt ttgtagtgaa atccgtcagg taaactgtta aagccaactt cttccaggtt
      181 aaccatatca tcagcctgtt gggatatcat actgatgaat tccattaaat caggctgaac
      241 gaccaattgc gccaaatatt ctcctccaag ggtatccggc atgatgacat gattggctcc
      301 tgcattgcgg atttttttca tggaagagcg atctgatgct ctggacacaa tatttaatga
      361 tggattgata gtatgtgcag aaacaactac aaataaattg tctgcatcag agggcaatgc
      421 agcaatcagt gtatgcgctt tttcaatccc tgcttctgcc agagtctcgt cctgtgttgc
      481 gtctccctga atgatcagca aatcatcgcg acgttcaaat ataattccgg aaatgttttt
      541 ttcgataaca acaaccttgt gattcccctt tagcaagcgt tctacagttt gcgatccaac
      601 acgaccgtat ccacaaacaa ttacatgatc tttcatagcg gctagaattt ttcttttttg
      661 ctgtttaaat aaatcctctc ggtaataccc atccaataca taggaactga tacttgcagc
      721 agcaacacca ataattacaa aactggttac aatcataccg ctaacaaata gacggccttc
      781 gggactcaat ggttcaactt cgccaaatcc aactgtagaa atagttatca atgtcatgta
      841 aaatgcatct ataatggaat acccttctag tgcacataat ccaactgtac ctagtaatac
      901 aatccctaag attgcaaaca aataatatct gaatcggtaa aaaagtaaaa accccattga
      961 aggaattaaa tatcccagat actccgctta cgggagccgg gtttgaagta tcgtttattc
     1021 tccagaataa aggccataat gagataaaga accaatggtg aacctacggt aataaatgaa
     1081 agataaataa aggatttacg cacccttaat gtatcaatgc caagtttatg accccaccaa
     1141 ttacaaacac cgaaagcctg acgttcaaaa tacgcctgaa ttttattgat catacctgtt
     1201 ttcaagcaat tgttttccta tagtgcaatt taagcatttt ttaggcccgc aatacatgtt
     1261 ccacatctca atttgtgatt gggtatccat ggcattcaga gcctttctgc ctgcgtgtct
     1321 gaaccgggtt gttatcctgt ttttttcagg catcacttct tcaagtaatt ccaatgcaga
     1381 ttcagaatac cgatccactc ccttgtccct gccataacaa aaaagcagag gaaccaccga
     1441 attgataatt atattctgcg atgaatccag ccccatacgt ttggggttca aacgcgataa
     1501 tttcccaaac ctgtaatgac gcatccaata atcggaaggt gttagccgga gccgctcgct
     1561 ccattcttct gcacctgtcg atcgcaaaag cattgcttcg agattggttt cttcatgcat
     1621 taaaattgca aactgtgcca agcgaatggt gggaaagttt accggtctca ttcgggaaaa
     1681 tttccaaaca gggcgttcga atacgggcaa ttgcaaacgt tgctgcacca gatcatatcg
     1741 aattgtaagg ttgcgctggt attcatctgc atcctcctga tccaggaatc cggcttgacc
     1801 gaacaacaat gactccatat acatccgatc accgcttacc ttacgaacga agtgatgcgg
     1861 agtatttcgt gctaaccatt caaaaggaag cgtgttaacc ctgaaaccaa aattccgtgc
     1921 aagcgcgcga taaatgactt cgttccaatt gttattgcac tccgcaatat cacgccgaat
     1981 cggaattatt tttcgttcca aacgctcggc taatgccctg tcaaattgtg aacgaataag
     2041 cacttccggt aactcagaaa attgtccggt acatgaaaaa tcacggcgtc cccagatgaa
     2101 tgccttgtaa cgttcatacc cttcaggatc tattcggttg gataattcca tacacaacaa
     2161 ttgtctgcca cgggaattca tcacctcctc gtcgtgctga aaaacaacat gaagaatcac
     2221 attatcgtaa gcttcatcaa attggtgccg gtgctttctc cattcagaag cgcggacatg
     2281 aatttcaaca ttacctgccc atagagttga accaatccga atgcgcgcat caaaaaaatc
     2341 gggaccagaa tcactgttat gctgaccagg atgaaggatg aaaatagttt ctcctgaagt
     2401 agtttttaaa tcgcctgaag taaacaacct gtacttccag acatgatgca gataatcttc
     2461 ggtcatgatg caatatctga atcaagcaag aagacaacaa atcaataatt atgtgtagtg
     2521 aacggttcga ttgaaacaat tacaacaacg ctgccatttg ttgttgcatg ttcaatgctt
     2581 cttcacgtgc tttctctgca aaatcacttc ctgaggatgc atataaaata cttcgcgatg
     2641 cattgaccaa aagaccacaa tctttattca ttccgaagcg tgccacatct tcaagactcc
     2701 ctccttgtgc gccaacaccg ggtacaagta aaaaatgatc aggaacaatt tggcggattc
     2761 tctcaagcat agacgcctgt gtagctccaa cgacatacat catattcgaa gcattgcccc
     2821 attggcccga aacttttaat acacgttcaa aaagcttttc ttctacctga tccgccgggc
     2881 caacattgag cgtctgtaaa tctagcgcac ctttgttact ggtaagtgcc aatagtatca
     2941 cccatttccc ttcctttccc aaaaaaggtg ttacactatc ttcgcccata taaggagcca
     3001 ctgtaacaga atcaaaattc attttgtcga ggaatgcttt cgcatacata gcagaggtat
     3061 ttccaatatc ccctcttttt gcgtctgcta tggtaaaatg atcagggtat tttgtgttga
     3121 gataatcgat cgttttctcc aaagcaatcc aaccggaaac tccgagcgat tcataaaacg
     3181 cagtatttgg tttataggcg acacataaat catgtgttgc atcaataatt gctttattga
     3241 aattaaaaac cgcgtcttca tcttttgcaa ataacgcagg aagtttttgc ggatcggtat
     3301 caagaccaac acaaaggaat gatcctttcc ttcgaatctg ggtaataagt tcgtttcggt
     3361 tcattttatt cttctaatcc gctttttaat ttttccgcat tttctgccat gcgtaatgca
     3421 tctatgatct cttcaatatc accctccatg attgccgcca aattgtatag cgttaaacct
     3481 atgcgatgat ctgttactct gccttgtggg tagttataag ttctgatctt tgcagatcgg
     3541 tcgcctgtac taaccatagt ctttcgtttc tgcgcacgct cctcaagagc tttactcaat
     3601 tctctatcgt acaaccttga acgcaacact tttaatgcac gttcaacgtt tttgatctga
     3661 cttctttcat cctggcactc cacaaccaaa cctgatggta tatgcgttaa acgaacagca
     3721 gattctgttc tgtttacgtg ctgaccacct gcaccggatg cacgaaaaac atcttttttg
     3781 atatcgttca tgtttatttc aacatccacc tcatctgctt ccggtagaac agcaactgtt
     3841 gctgctgaag tatgtaccct tccctgcgct tctgtttcgg gaactcgctg aacacgatgc
     3901 acgcctgatt cgaatttcag aaaaccataa acctcctcgc cagaaaccag taatgaaacc
     3961 tccttgtacc ctcctacttc actttcactt actgacatca cttctacttg ccaccctttt
     4021 tttgcaaaat aacgtgtata catccggtac aaatcgccag caaaaattgc ggcttcatct
     4081 ccacctgtac ctgaacgaat ctcaacaact gcattcttcg aatcttccgg atcttttgga
     4141 atgagcaaaa cttttatctc atcttccaat tcagctttac gttgctgcaa ggagtcaagt
     4201 tcagcttttg ccatttcacg aaatgcttca tccttttctg tacgcaataa gtccagcgtt
     4261 ccatcaatac cctcaatagt cttttcgtat tcgtcgcttg cgcttacaat tggacccaaa
     4321 tctttgtatt ccttattcaa acgaacatac ctcttcatat cagcaatcac tgccggatcg
     4381 gtaatgagtt cactcacttc gcggtagcga tctgcaatgg caagtaattt tgaacggata
     4441 tccacttatt gaataattgt ttccattaat aaatgaattc tccaaatgga gaacgaatgg
     4501 tcagcttttt cttttccgaa tcttcaacgc gtccaacaac acgagcatca actccaaatg
     4561 aacgtgataa tgctattaat ccatctgcag ttttttcatc ggtgtaaatt tccatacggt
     4621 ggcccatatt gaaaacttta tacatttcac tccactctgt acctgattgt tcgtgaatca
     4681 ttttaaataa tggtggaaca gggaataaat tatcttttat tacatgaaca tcatcaacaa
     4741 aatgtaagac tttggtttga gcgcctccgc tgcaatgtac cattccaaaa atatgatgtc
     4801 gatattcagt aagcattgct ttaataattg gcgcataggt tcgagtcgga gaaagaacca
     4861 gcttaccggc agtcattttt tccctggtat tttcgtcgac tatgatctca tcagtaagat
     4921 tcaatccacc tgaataggca agatcctccg gtacagcagg atcatacgtt tgtgggaatt
     4981 ttttcgcaat cgatttattg aaaacatcgt gacgtgctga tgttaatccg ttgctaccca
     5041 ttccgccatt gtattccttt tcataattgg ctttaccaaa tgaagagagt ccaacaataa
     5101 catttcccgg agcaatagtg tggttagaca caaccgcatc ccgacgcatt ctgcacgtta
     5161 ctgttgaatc aactataatt gttcgaacaa gatcaccaac atcagccgtt tctccgccag
     5221 ttgaccaaat gccgatgcca tacgagcgca attcttcaag taactcttcc gttccgttta
     5281 tgatggcgga aatcacttca ccaggaatca gatgcttgtt tcttccaatg gtggaagaga
     5341 gtaaaatatt atcgacagca ccaacacaga gtaaatcatc aaggttcata acgattgcgt
     5401 cctgcgcaat tcctttccat acggatagat cacctgtctc tttccagtat gcgtaagcaa
     5461 gtgaagattt tgttcctgcg ccgtcagcat gcatcacaac acaatgttct tcactacctg
     5521 taagcagatc cggaactatt ttgcaaaatg cctttggaaa caagccttta tccaggtttg
     5581 caattgcatt atgaacatct tccttggagg ccgaaacacc tcgctgattg tagcgattat
     5641 cagacataga gtacaaaaat aagaaaagca tccgctgcgg cggatgcttt tccaaatgaa
     5701 tacatttcga ttaatcttct ctgcgcttca tccagggaag gtcgatccat tgaagtttca
     5761 aatcagcttc agtgggaaca taatcgaagt taaggatctt aaagtctgta cgacggttca
     5821 tctggtgagc agcgttttgc tcttcaactg aggtcatttt cttgatgaaa ggttcgctca
     5881 accaaacagt atcacgacct acaaccttat aagttggttc cttttcaccc ataccttttg
     5941 ccaccagtcg ctcttttgga atccccttac ttacaaggaa atctacgcaa gtttgagcac
     6001 gacgctgaga aagtttttca ttataggcat cgtttccgcg gaagtcggtg tgtgaacgaa
     6061 gctgtacttt gatagttgga ttttcaacca tcatattgta caggtaaagc agtgaatcct
     6121 gagagtttac aggacgcttt gtttcgtttt cttttttggt tccttcaagg tcaacaataa
     6181 gatctgcctt atcgtaagca taaacgatca taggaagacg caatggctta ccaacatatt
     6241 ttttcatgta gaatgtgaat tcccagttgg tggacttatc aactccaaca gtggtgaatt
     6301 gtttcttaag ctccatactt ttcaggaagc ctttaggaag agttgcttca actgtgtaat
     6361 tggtcgcttc attcacataa cgctttcctt cagcagcagt aataaatgat actgaaccgg
     6421 catcgttggt ttttaagctg aacatggtgt tgtcagttcc tgccaacttc acttcaatat
     6481 ccttgatcgg ttgttttgtt tcttcatcga gaacaaaaat cttcaattcg tacaacagcg
     6541 gaggcataac gaaactatac agatcatcac gtcctttacc accaacgcgg ttagaagtaa
     6601 agaacccttt atcttttcct tcgaatacaa ttccaaaatc gtgttgcggt gaattcatcg
     6661 ggtaaccgag gttctttggg ttctcccact ttttctcagt tcccacagct tcggcacgga
     6721 acatatccaa acctcccata ccaaggtgcc catctgaaga gaaataaagc gatccgtctg
     6781 aatgaatgaa agggaacatt tcatcacccg gggtattgat ttttggacca agatttacag
     6841 gatcactcca ggattttgca cgcttatcat acgttaccat ccaaagatct tttccacctt
     6901 ctccacctgg catatcagaa gcaaaaatca aggccatgtc atctccagta agagcagggt
     6961 gccctactga aattgaatcg tgtggcttga gggaaggtat ttttgtagga gttccccact
     7021 tatcaccttt tctttcaact acaagaatgt cgcaaccaag gttctttttc ttttcgttcg
     7081 gacagtttgt gtaatacatt acatcacctt tacgatcaag aactgcagct ccttcgttat
     7141 cgtcggtatt aattccttgc ggaagaattt ttggctctcc ccattttcct tttttatcac
     7201 gttctgttac ccaaagatca gttctacctc caccggtacg cttttcttca atagtaccta
     7261 caccaccagg acgagaagat gaaaacacca gagtggtatt tttcttgtcc atgaacatgg
     7321 gtgtaaagtc ataatattct gtattcagga taagttcatt ttcaatgatg tggcgagttg
     7381 gtgttttttt ccactcttgt gccaacttac aggcctcagc accattcttt ccctgaacat
     7441 caccgctctt ttggaaatag gtggtgtagt tcttttccgc ttctttgtat ttaccctgtt
     7501 cgcgctgtgc atctgcaagg cgaaggtata cgatggcatc tttgtagttt ccatccacac
     7561 aacgctgata aaactcttca gccttatcta cgtcggtaag gaatcgataa cattcaccca
     7621 cacggaaata atagaacatt tttttctctg gcttcttctc tttttcagcc gcgaccttat
     7681 aaaggtcctt agcagtaaag aactgctcat tcaaataggc agcttcggct tctttgccat
     7741 ggttcttttg cgctgacgca ctgagagtta ggactaatg
//
LOCUS       DAOMYG010000100         7755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3131_length_7755_cov_8.018442, whole genome shotgun sequence.
ACCESSION   DAOMYG010000100 DAOMYG010000000
VERSION     DAOMYG010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7755
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3131_length_7755_cov_8.018442"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /gene="lpdA"
                     /locus_tag="PK610_04605"
     CDS             <1..1217
                     /gene="lpdA"
                     /locus_tag="PK610_04605"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRJ35242.1"
                     /translation="EYIQHAADYGISVGNAKADFNGMIKRSREVANGMSKGIDFLMKK
                     NKIDVIKGTGKLKPGKKLDVTGEDGKVTTYEAKNIIIATGARSRQLPNLPQDGKKVIG
                     YREAMSLPNQPKSMVVVGSGAIGVEFAYFYNSIGTKVTIVEFMPTIVPVEDEEVSKQL
                     ERSFKKQGIDIMTEASVEGVETKGNGCVVKIKTKKGEEKIECDIVLSAVGIQANIENI
                     GLEEVGIVTDKGKILVNEYYTTNIPGYHAIGDVVPGPALAHVASAEGIICVEKIAGHH
                     PEALDYGNIPGCTYCNPEVASVGMTEKQAKEKGIEIKVGKFPYSASGKASAAGAKDGF
                     VKLIFDAKYGEVLGGHMIGANVTEMIAEIVAVRKLETTGHDLIRTVHPHPTMSEAVME
                     AAAAAYGEVIHL"
     gene            complement(1299..1667)
                     /locus_tag="PK610_04610"
     CDS             complement(1299..1667)
                     /locus_tag="PK610_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35243.1"
                     /translation="MDVKKQLSLVGTGMVLVSCFLPFISFFGMSISLFGASAGEALLV
                     IILGAAAGGLDFMGQKQGNKMFIIIALVAAVLGLLLSLYWMMKASGMAGTGLYVMVAG
                     FAVALAGTAMSFKNFGAAKN"
     gene            complement(1788..3272)
                     /gene="dacB"
                     /locus_tag="PK610_04615"
     CDS             complement(1788..3272)
                     /gene="dacB"
                     /locus_tag="PK610_04615"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HRJ35244.1"
                     /translation="MRYSIVVVFVCCIPFLAVAQQIKDTTKNNVEKAVEEFLKDPDLK
                     NIALTFHAVEVSTRKAVASYNAEMSIVPASCMKLITTATALEILGGGYKFKTVIQYSG
                     TLDTVKRVLNGDIFILGGGDPTLGSKYFAKDDPSAFLIDWARAIKAAGIDSITGNIIA
                     DAEIYGWEMVPSTWAWGDLGNGYGAAPCGVSIYDNTCVLKFNTGSKAGDTARIECVMP
                     YVPDLEFSSDVYAGNTTDDNAYIFGAPYSYQRVVKGVLPKGKEAFEVKGTIPDPSYLA
                     AFELMHALLAEGIAVGGRPNTVREMKMKGAFTSPVRTDIYTHYSPAVASIVNLTNTFS
                     VNLFAEHLINQIGLIKYGNGTVGSGAAAMLEFWAKKGIDTKGLYITDGSGLSRFDAVS
                     ASHFVQILLWMTQSKSFSTFEKSLPVAGRSGTLSGFCKGTRAEGKVAAKSGTMTRVKA
                     YSGYVTSKAGKRIAFSVVVNNFNCSTKQMEKKLEKIMVALVDYV"
     gene            3347..5206
                     /gene="asnB"
                     /locus_tag="PK610_04620"
     CDS             3347..5206
                     /gene="asnB"
                     /locus_tag="PK610_04620"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRJ35245.1"
                     /translation="MCGITGAFQPTGISSTTSAEVRSALNELRHRGPDNHSFFDSPNC
                     CLGHARLSIIEPSAASHQPFYSKDKRYVIVFNGEVFNFRELRKSLQDLGIDFTTDSDT
                     EVVLYGLILEGTNFLNKINGFFAFAFYDQHSNEILLARDRFGEKPLYWNTSQNGLFFA
                     SELGALIKYAFKKTIDKSALWLYFRLSYIPAPHSIFNEIKKLEPGYFIKSTINGITSK
                     KWYKPESKNLLNNKAEAREKLAVLLENAVQRRLIADRPIGAFLSGGIDSTIVCGIAAK
                     QMDQLNTFSISFPEYPYFDERMYAESAAKHSGTNHTTIPIGKTELANAIDPVLNQLSE
                     PFADSSSVPMFVLSREVGQHIRVALSGDGADELFGGYNKHRALLRSLSASTANSLAGF
                     AQYFIPSALTSRNGKWKDRLRLIKKYGKGLQLNVRNRYFEWSGFSDESLVGQLFTPEY
                     CGAENELLIQHCTEAITHGTMEEFLLNDQALVLTNDMLYKVDQMSMAHALEVRSPFLD
                     HEIVEFAQQIPAEWKLNAKQGKLILRETFKEYLPESILNRSKRGFEVPLEEWISTDLQ
                     MRIKEEWLSADYIKKQGIFNPSTIQNILHEPQLNAHLIWMLVVFQSWYRRNCE"
     gene            5208..6368
                     /locus_tag="PK610_04625"
     CDS             5208..6368
                     /locus_tag="PK610_04625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ35246.1"
                     /translation="MPRVLRIINRFNLGGPTFNAAYLTKFLEPEFETLLIGGLKDESE
                     AGSEFILENLGIQPMILEEMRRAPGLSKDYKTYKRIRAIIKEFKPDIVHTHASKAGAI
                     GRLAAHHEGVKAIVHTFHGNVFKGYFGATKNILYKNLERYLAKRTHAIIAISDLQKRE
                     LAEEHRICAAEKIRVIPLGFDLLKFKTEKEKKRKAFREKWSIHENEIVISIVGRLVPI
                     KNHKLFIDAMRLVAEQSNIPVKAMIVGDGESRASLEEYIKVNCPKDHPFVFTSWIREV
                     DEVTAGSDIIALSSLNEGTPVSLIEAQAAGTPIVSTDVGGIRDIVIPEKTALLSPSED
                     AESFAFNLLRLINQPSLRSEMSEQGWPHVEDRFHYSRLVKDMRQLYLELLKA"
     gene            6409..7173
                     /locus_tag="PK610_04630"
     CDS             6409..7173
                     /locus_tag="PK610_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRJ35247.1"
                     /translation="MIYRRLLVLALFALSLPGCYINSNIMLRTDRNYVFDSIPDTVGR
                     AYKISPNDIINFRLFANDGFRLIDIASGLDNDQGNRAMFRNFMSYLVEGDGTAKLPIL
                     GKVPLSGLTVREAEFKLETMYSEFYVKPFVQVQVTNKRVIVFPGGGGNAAVITLENNN
                     TTLMEVLAMAGGIAQRGRAARIKVIRKIDNVRHVYLVDLSTIEGIRHADMIVQANDYI
                     YVEPVPQIANEILRDVAPIISLLTSAVLIYTVVQRL"
     gene            7183..>7755
                     /locus_tag="PK610_04635"
     CDS             7183..>7755
                     /locus_tag="PK610_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35248.1"
                     /translation="MIDEKIQRQSESFSQYKERITNFSNEFELGLFLYIARKSLRWVL
                     LFLITSLLAAWLYLRYTPPVYESLSVIQISEENNMKQVIPGINSFEDEQSGVDKALEL
                     MRSEFLFKKSIEKLDFTVSFFSEGQILTENKYPAKYLRFSDLTIIDSTIFGERIDIFS
                     NSNQITLNCRNQTFTTESGGLLQSPWFRARI"
BASE COUNT         2351 a         1689 c         1607 g         2108 t
ORIGIN      
        1 ttgaatacat ccagcacgcc gccgattatg gaatttcagt tggaaatgca aaagctgact
       61 tcaacggaat gatcaaacgg agtcgcgagg ttgccaatgg aatgagcaag gggattgatt
      121 tcctgatgaa gaagaataag attgatgtca ttaagggaac cggaaaactg aagcccggaa
      181 aaaaacttga tgtaaccggt gaagacggaa aggtgacaac atacgaagcc aaaaacatca
      241 ttattgctac cggagctcgt tcacgtcaat tacccaacct tccgcaggat ggcaaaaaag
      301 tgatcggata ccgtgaggcg atgtccttac ccaaccaacc aaaatcgatg gttgttgttg
      361 gttccggtgc tattggtgtt gaatttgctt atttctataa ttccatcgga acaaaagtga
      421 ccattgttga gttcatgcca acgattgttc ctgttgaaga tgaggaagta tccaaacaac
      481 ttgagcgttc cttcaaaaag cagggtattg atattatgac tgaagcctct gttgaaggtg
      541 ttgaaaccaa aggcaacggc tgtgttgtaa aaattaaaac caaaaaagga gaagaaaaga
      601 tcgaatgcga tattgttctc tctgctgtgg gaatccaggc gaatattgaa aacatcggac
      661 tggaagaagt aggaatcgta accgacaagg ggaagattct cgttaatgaa tattacacca
      721 ccaatattcc cggttaccat gcaatcggcg atgtagttcc cggtcctgca cttgctcacg
      781 tagcttctgc agaaggaatc atttgcgtag aaaagatcgc aggtcatcat cccgaagcat
      841 tggattacgg taatattccc ggttgtacct attgcaatcc ggaagttgca tccgttggaa
      901 tgactgaaaa gcaggctaaa gaaaaaggca ttgaaataaa agtcgggaaa ttcccctact
      961 ctgcttcagg aaaggcttct gctgccggag cgaaagatgg ttttgttaaa ctcatttttg
     1021 atgctaaata cggtgaagta ttaggaggac acatgatcgg agccaacgtt acggaaatga
     1081 ttgcagaaat tgttgccgta cgtaagcttg aaacaaccgg ccatgatcta atccgtacag
     1141 tgcatccgca tccaactatg agcgaagcag tgatggaagc cgcagctgct gcatacggcg
     1201 aagtgattca cctctgataa aatgtaaact caaaaaaaat ccccttcgca aaacgaaggg
     1261 gattttttta tgcgctcaat gagcgttgat tgtcaatttt agttctttgc agcaccaaag
     1321 tttttgaatg acattgcagt ccctgcaaga gcaacagcaa aacctgctac cattacataa
     1381 agacctgtac ctgccatacc gctggctttc atcatccagt aaagagaaag taacagaccc
     1441 aatacagctg caactaaagc aataatgatg aacattttgt taccctgctt ctgacccata
     1501 aaatcaagtc cgccggcagc agcaccaaga ataataacta aaagagcttc acctgcagaa
     1561 gcaccgaaaa gagaaatgct cattccgaag aaactgatga aaggaagaaa gcagctaaca
     1621 agcaccatcc cggtacctac taaagacaat tgttttttaa catccatgtt gtatgatttt
     1681 ggttttggtt acaagagaga gttgaaattt gtgctcctaa attataaaaa atgaacaatt
     1741 tgtgaagcga ctaaaaaaaa atcaggcgaa aggttattaa caaattccta cacataatcg
     1801 accaatgcga ccatgatctt ttcgagtttc ttttccatct gtttggttga acaattaaaa
     1861 ttattgacca caaccgaaaa cgcaattcgc ttcccggctt tgcttgtaac atatcccgaa
     1921 taagccttaa ccctggtcat ggtaccactt tttgcagcca cctttccttc ggctcttgta
     1981 cccttacaaa aaccggaaag tgtaccactt cgaccagcta ccggtagtga tttttcaaat
     2041 gttgaaaaac ttttactttg cgtcatccat aaaagaatct gaacaaaatg tgaggccgaa
     2101 accgcatcaa aacgtgataa accacttcca tcagtgatgt acaaaccttt cgtatcgatg
     2161 ccttttttgg cccaaaactc cagcatcgcc gcagcacctg agcctaccgt tccattaccg
     2221 tattttatca ggccgatttg attgatcaaa tgttcggcaa agagatttac cgaaaaagta
     2281 ttcgtgaggt ttacaattga agctactgcc ggagaatagt gcgtgtaaat atcggttctt
     2341 accggagagg tgaatgcacc cttcattttc atttcgcgaa ccgtattcgg acgaccgcct
     2401 actgcaatgc cttcagcaag gagagcatgc atcaattcaa atgcagcaag ataactggga
     2461 tcaggaatcg ttccttttac ctcaaaggct tcttttccct ttgggagaac tccctttaca
     2521 acccgttggt aggaataagg agccccgaaa atgtaggcgt tgtcgtcggt tgtattgccc
     2581 gcatacacat cacttgaaaa ttccagatca ggaacataag gcatcacaca ttcgatgcga
     2641 gctgtatcac ccgctttact acctgtatta aacttcaata cacaagtatt atcataaatg
     2701 gaaacaccac aaggagcggc tccataacca tttcccagat caccccaggc ccaggtggag
     2761 ggaaccattt cccaaccgta aatttcagca tcggcaatga tgttaccggt gatggaatcg
     2821 atgcctgcag ctttaatagc ccgcgcccag tcgataagaa atgctgatgg atcgtctttt
     2881 gcgaaatatt ttgaccccag tgtaggatct cctcctccta aaatgaaaat atctccgttc
     2941 aggacgcgct ttaccgtatc caaggttccg gaatactgaa tgactgtttt aaacttataa
     3001 cccccgccca aaatctcaag tgctgtggcg gtcgttatta gtttcataca cgatgccgga
     3061 acaatactca tttccgcatt gtaggaggca acggctttac gcgtggaaac ttctactgca
     3121 tgaaaagtta atgcaatgtt tttcagatcg ggatctttta aaaattcctc aaccgctttt
     3181 tccacattgt tcttcgttgt gtcctttatc tgttgtgcaa cagctaaaaa cggaatgcaa
     3241 caaacgaaaa ctactacaat cgagtaccgc atgataagtc taaatatact atgttttctg
     3301 ttactgaact gactttgtta tctttgtcca tgcaaatgct gtgcccatgt gcggaataac
     3361 cggagctttc caaccaactg gaatcagcag taccacctct gcggaagtcc gctcagccct
     3421 gaacgaactg cgccatcgtg gccccgacaa ccactccttt ttcgattcgc cgaattgttg
     3481 cctcggtcat gcccggcttt cgatcattga accctctgcg gcttcgcatc aaccctttta
     3541 ttcaaaagat aaacgatacg tgatcgtatt caacggtgaa gtatttaatt tccgtgaatt
     3601 acgaaaatcc cttcaggatc tgggcatcga ttttacaacg gattccgata cggaggtcgt
     3661 tctgtacggg ttaattctgg aaggaacgaa ctttctgaat aaaataaacg ggtttttcgc
     3721 cttcgcattt tatgatcagc atagcaacga aattctcctt gcgcgtgata gattcggaga
     3781 aaaacctttg tactggaata cttctcagaa tggcctcttt tttgcatccg aattaggagc
     3841 cttaataaag tatgccttta aaaaaacgat agataaatct gccctttggt tgtatttccg
     3901 tttgagttat atacccgcac cacacagtat ttttaatgaa ataaaaaaac tcgaaccggg
     3961 ttatttcatc aaaagcacca tcaatggaat taccagtaaa aaatggtata agcctgagtc
     4021 aaaaaacctg ttaaacaata aagccgaagc gagggagaaa ttagcagtcc tgttggaaaa
     4081 tgcggttcag cgaagactta ttgctgatcg tccgattggc gcatttttaa gtgggggaat
     4141 tgattcgacc attgtttgtg gtatagctgc aaaacaaatg gatcaactga atacattttc
     4201 cataagtttt ccggaatatc cttatttcga tgaacggatg tatgccgaaa gcgctgcaaa
     4261 acattcgggc accaatcaca caactattcc cattggaaaa accgaattgg ccaatgccat
     4321 tgatcctgta ttgaatcaat tatcagaacc ctttgcggat tcatcttctg ttcccatgtt
     4381 cgttctgagc agagaagttg gtcagcacat ccgtgttgca ttatccggcg acggagcaga
     4441 tgaattattt ggaggctaca acaaacaccg tgcattatta agatcgcttt ccgcctctac
     4501 cgcaaattct ctggccggtt tcgcccagta ttttatccct tctgcactca catccagaaa
     4561 tggcaaatgg aaagatcgat tgcgattgat aaaaaaatac ggaaaaggat tgcaacttaa
     4621 tgtccgcaac aggtattttg aatggtcggg attttctgac gaatcactgg taggacaatt
     4681 gtttacaccg gaatattgcg gagctgaaaa tgagctgctt atccaacatt gtaccgaggc
     4741 gattacccat ggaacaatgg aagaatttct gttgaatgat caagcactcg tcctcaccaa
     4801 cgacatgttg tataaagtcg accagatgag tatggctcat gcattagagg ttcgttcgcc
     4861 cttccttgac catgagatcg tagaatttgc tcagcagata cctgccgaat ggaaattgaa
     4921 tgctaaacag ggaaaactca ttcttcgcga aacctttaag gagtatttac cggaatctat
     4981 tcttaacaga tcgaaaagag ggttcgaagt acctctcgaa gaatggataa gtactgatct
     5041 tcaaatgaga ataaaagaag aatggttatc tgctgattac attaaaaagc agggaatttt
     5101 caatccaagt actatacaaa acatccttca tgaaccacag ttaaatgcgc acctgatctg
     5161 gatgttagtt gtttttcaat catggtaccg tcgaaattgt gaatgatatg ccaagagtac
     5221 tgcgaataat caaccggttt aatttaggtg gtccgacatt taatgcggcc tacctcacca
     5281 aattccttga acccgaattt gagacgcttc tcattggagg attaaaagat gaaagcgaag
     5341 caggcagtga attcattttg gaaaatcttg gcattcaacc aatgatcctg gaagaaatgc
     5401 gaagagcacc cggactaagc aaagattaca aaacctataa gcgaattcga gccatcatta
     5461 aagaatttaa accggacatt gtgcatacgc atgcttccaa agccggagca ataggtcgtt
     5521 tggcagcaca tcatgaaggg gtgaaagcca ttgtacatac tttccatggt aatgtgttta
     5581 aaggatattt tggcgctacc aaaaatatct tgtacaaaaa cctcgaacgt tatcttgcaa
     5641 aacggacaca tgccattatt gccatttctg atttacaaaa aagagaatta gcagaagaac
     5701 atcgcatttg tgcagctgaa aaaattcgtg tcattccact tggattcgat ctcttaaaat
     5761 tcaaaaccga aaaagaaaaa aaacgaaaag cattcagaga aaaatggagc atccatgaaa
     5821 atgaaattgt gatttccatt gttggtcgtc tggtccccat taaaaaccac aaactgttca
     5881 tcgatgcgat gcgtttggta gctgaacaat ccaatattcc tgtgaaagca atgattgttg
     5941 gagacggtga atcacgcgct tctttggaag aatacatcaa ggtcaattgc cccaaggatc
     6001 atccttttgt ttttacctca tggatcagag aggttgatga agtaacagcc gggtcggata
     6061 ttattgcttt aagctcccta aacgaaggca cccccgtaag cctcattgaa gcccaggccg
     6121 ccggaacgcc aattgtaagt acagatgttg ggggtatccg ggacattgtc atccctgaaa
     6181 aaacagcgct attatcgcca agcgaagatg ctgagtcctt tgcattcaac ctcttgcgac
     6241 taatcaacca gccatccctc agatccgaaa tgagtgagca gggctggcca catgttgagg
     6301 atcgctttca ttacagtcgt ctggttaaag acatgcgtca gctctatctt gaattgctaa
     6361 aagcctgata gattgccctg ttttgcttac ttttggaaaa aaaacacgat gatctacagg
     6421 cgacttctcg tccttgctct ttttgcgctt tcactaccgg gctgttatat caactcaaac
     6481 attatgttgc ggaccgaccg caattatgtt tttgattcta ttcccgacac cgttgggcgt
     6541 gcctacaaaa tcagtccgaa cgacatcatc aatttccgat tgtttgccaa tgatggattt
     6601 cgtcttatcg atattgcttc aggattggat aacgatcagg gaaaccgcgc catgtttcgg
     6661 aatttcatga gttacctagt tgaaggcgac ggaacagcta aacttccgat ccttggaaaa
     6721 gttcctcttt ccggattgac agtgcgtgaa gcagagttca aactggaaac gatgtattcc
     6781 gaattttatg taaagccatt tgtacaggta caggtaacca acaaacgggt aattgtattt
     6841 cccggaggtg gaggaaatgc cgctgtgata acactggaaa acaacaatac cacattaatg
     6901 gaggtgcttg ccatggcagg tggaattgca cagcgtggaa gagcagcccg cattaaagtg
     6961 attcgcaaaa ttgacaatgt tcgtcatgtt taccttgttg acctctccac catcgaaggt
     7021 attcgtcacg cagatatgat cgtgcaggca aacgattaca tatacgtaga acccgttcct
     7081 caaatcgcga acgaaattct tcgggatgtt gctcctatca tttccttact taccagcgca
     7141 gtgttgatct atactgttgt tcaacgactg taatcctttt tcatgatcga cgaaaagatt
     7201 cagcgacagt ccgaaagttt ttcccagtac aaggaaagaa tcaccaattt cagcaatgaa
     7261 tttgagcttg gtttgttttt gtatatcgcc aggaaatccc tgcgttgggt tctattgttt
     7321 ttaatcactt ctctgctcgc tgcgtggttg tatcttcgtt acacacctcc tgtttatgaa
     7381 tcgctgagtg ttattcagat cagtgaagag aacaacatga agcaagtcat tcccggcatc
     7441 aattcatttg aggatgagca atcgggagta gacaaagcac tggaactaat gcggtctgaa
     7501 ttccttttca aaaaatcaat tgaaaaactt gattttactg tcagcttttt ttcagaggga
     7561 caaattctca ccgaaaacaa ataccccgct aaatacctcc gcttttctga tctaaccatc
     7621 attgactcta ctattttcgg ggagcggatt gatatttttt ccaatagcaa ccagatcact
     7681 cttaattgca gaaaccaaac tttcactacc gaaagcggag gattacttca gtcgccatgg
     7741 ttcagagcta gaatc
//
LOCUS       DAOMYG010000101         7688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3174_length_7688_cov_9.938949, whole genome shotgun sequence.
ACCESSION   DAOMYG010000101 DAOMYG010000000
VERSION     DAOMYG010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3174_length_7688_cov_9.938949"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..2291
                     /locus_tag="PK610_04640"
     CDS             63..2291
                     /locus_tag="PK610_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ35249.1"
                     /translation="MKRFLLAIVLLCLAGFAQNAGAQQFYQEEFDGTVCAAGSGCDPS
                     LVGWSVTNTGTNGANANRFFISCQENGNNAGQCGSGCGSDQSLHIGNVSTSLAAFIFC
                     PTGDCGAAYDDSSPAEATDKRAESPVISCVGQSNITCTFVYMENGEGSNDDASFWYFN
                     GATWAQLDPIAKSPLTCAPQGTWTNYSVTLPASANNNPNVRIGFRWVNNGNGNASDPS
                     FAVDDIRLISNPTTPPLAAFTQSATNICAGDPVTFTDQSTGGPTSWSWSFPGGTPATS
                     TAQNPVITYATPGTYSVTLTVTNGNGSNSTTSTNLITVTTCTSPPTAAFSASLTTICS
                     GDAVNFTDLSTGSPTSWSWTFTGGTPATSTVQNPSVTYATPGTYTVTLTVTNANGSDT
                     ETITNMITVNNCSTPPTAAFSANTTAICIGQQVTFTDQSTGTPTGWSWSFPGGTPATS
                     TLQNPVITYSTPGTYDVTLTVTNSGGSNSTTTTNLIVVSNCTVPVASFTPSATNLCAG
                     ACVTFNNTSTDATLYNWTFPGGTPSSSTLQNPGTICFNTPGSYQVVLTVSDGITSSST
                     TSTITVNPNPTVFANADTTIDIGNSAILTAVATGLGTYIWTPSTGLSSPGSATTTAAP
                     LTTTTYTVTFTENGCSATDQVTVFVNVIEGIGVPNAFSPNNDGINDVLMVYGQGIQGV
                     TFIVYNRYGQKMFETNDPNIGWDGKYDQKMVNTGVFVWYLEYSLVSGASGTLKGNVTL
                     TR"
     gene            2310..3326
                     /locus_tag="PK610_04645"
     CDS             2310..3326
                     /locus_tag="PK610_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRJ35250.1"
                     /translation="MQKVKIIFSAMLLGWSVQVFGQQDIHFSQFYTASAYINPATAGM
                     FNGDIRGVMNYRSQWKSVSEPFTTFTGSVDARVANGSEGFLSIGGVFYNDKAGSAKLT
                     SGQYGLNIAWAAKLKEGMYWTFGVQPSLLTKSINGADLYYGNQWIGTEFSSAVANNET
                     FVNDKFMAFDVSAGSYWLYRPHKETAYYLGLAGAHLTRPNVSFLGTKDKMLMKFTFHG
                     GAEFELSNSNMAILPNFLVKMQGVNRIINFGSDVKYIIQKQSHYTGYSNEISAGLGAY
                     YRVGDAFWGALQFNWTGFTLAFSYDINLSNLSVATNGNGGMEMMLMYRAGIGAGKGKS
                     TRLL"
     gene            complement(3365..4183)
                     /locus_tag="PK610_04650"
     CDS             complement(3365..4183)
                     /locus_tag="PK610_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35251.1"
                     /translation="MKKLSTLLFVALFSFNAEAHYEKPLFSYSFSYLNNYPLSAMRPQ
                     LDGVNGFDIHAYGPLKGSRLQLGFSFAYGNYGNKNSKIDFATQDGSRFIADMTISNYY
                     LSPNMRFKYDFRPYASAVRPYAEVTGGVTFFQTSLNIWDPEDETNCEALETHFLKKDN
                     TWTTYAGAGVEISLTSLINKGEHSDCAMDFSVFFSGGFRTGGKVEYMSVDMDDQNLQT
                     HAGHHGTSADKSPFNVDFVNTVTQERHQHHIAYVFRSPIMMYEFQFGIRMHFGK"
     gene            complement(4217..7669)
                     /locus_tag="PK610_04655"
     CDS             complement(4217..7669)
                     /locus_tag="PK610_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HRJ35252.1"
                     /translation="MKKAAAFFFCVVAFPIFAQHKSIHQEQSETYATAGHSAEDYYSI
                     NSPSPKTRVLRNNCTPNKIIYGWHPYWSNGLQSNYDWDLLTHFSYFSYEVNYVNGNAN
                     NTYNFATTQSVTDALNNGLKVTLCVTLFSDHATFLNNNTSKQTLITNLINLISARGAH
                     GVNIDFEGLPASQSLNFRNFMNDLANQMHSAIPGSEVSTVLYAVDWNNVLDVANMTAV
                     DHFIIMGYDYYWTGSTTAGPNDPLFQFGTSYNYTLSKSITYYLNKGIPPAKLILGLPY
                     YGREWPVSSHTLPATTTGSGLSRTYRSVRDNVPGIFSAANRRYEPASKSTYYNFNSGG
                     LRQCFITEADDMRDRMDLINKRGIGGMGIWALGYDDGYNDFWDAISDNFTDCSTIPCS
                     DTLTDIGGGRFKNYYDNENYTYTIAPPNAGSVTLTFSSFSLENNYDFLYVYNGPGTTS
                     PQFPGSPFTGTTLPPALTGTTGAITLRFTSDNSTTSAGFVATYQCTSDNQPPTTGINA
                     SNNWKTANFSISFTDQDAGSGVARSFWQVMDNNGTEWRANGSHGFFNDNFSGTIHSDW
                     TQQSGTWIINSGRLAHTDESNGNTNIFAAVTQTSSYQYLYHWQGKMSGTGTNRRSGIH
                     FFCDNPSLPNRGNSYFVYFRVDQNKCQIYEVVNDVFTLMTDHSISIAANTWYDYKITY
                     DPTSGTIKAYVNNVLVSQWTDSTPLIAGNSISLRSANVNMEYDDVKVYRSRTTTENVS
                     LGSSSAMVRYQNNGPANSSCRIRTMIIDNANNWSNSASQDQNIDWTPPTTPAYLQEGI
                     SVDIDTTHENLNRSASWGSSNDPHSGIQTYSISIGTTPGDSSILNWQNTGNVTGDTIT
                     GINLVFGQSYYLNIRAVNGASLRSLLISSNGFYVTQPTAAPIANFTSGAMNACTAFPF
                     LIFNSSTNATGFLWNIPGGNPSTSTATHPEILFPSSGNYTVTLTANGPSGTDVLSQQL
                     TITLIDPPVASFSVNDTLLYLPGAYLAISNWSQNASSYSWNFGDSTFSTDPAPWHLYN
                     QSGIYQVLLLAENSVCPSDTAMATVYVFAPTGTNDAIAESGTIILNNQTLYIQWDIPQ
                     QLECGIELIDIQGKQILYSTPSGYSIQNGVSIHVPELSSGVYLCRLFFPTFVKTVKLV
                     FIQE"
BASE COUNT         2079 a         1628 c         1763 g         2218 t
ORIGIN      
        1 ggcaagtaaa aatctcttca tggccatagt tttctcctat atttataacg aaaactattg
       61 ccatgaagag atttttactt gccattgtgt tgttgtgtct tgccgggttt gctcaaaatg
      121 caggcgctca gcagttttac caggaggaat ttgacgggac tgtttgtgca gcaggttcgg
      181 gttgcgatcc atcacttgta ggttggtcgg tcaccaatac cggaaccaat ggtgccaatg
      241 ccaatcgctt ttttatcagt tgccaggaaa acggaaacaa tgccggtcag tgcggatccg
      301 gttgcggttc tgatcaatcg ctgcatattg gaaacgtatc cacatcgctc gctgcattta
      361 ttttttgtcc gaccggcgat tgcggtgctg cctacgatga cagcagtccg gccgaagcaa
      421 ccgacaaacg tgcggagtcg cctgtgatca gttgtgtggg gcaatccaac atcacctgca
      481 cattcgttta catggagaac ggagaaggaa gcaacgacga tgcctcattt tggtatttta
      541 acggcgctac atgggcgcaa ttagatccca ttgctaaatc gccacttacc tgtgcaccgc
      601 agggtacctg gaccaattat tccgtaacac ttcctgcttc tgcaaataat aatcccaatg
      661 tccgaatcgg ttttcgctgg gtgaacaatg gaaatggtaa tgcatccgat ccatcttttg
      721 cggttgatga tatccgattg atttcaaatc caaccacacc accccttgct gcatttacac
      781 aaagtgcaac aaacatatgc gcaggcgatc cggttacgtt tacggatcag tcgaccggtg
      841 gtccaacgag ctggagttgg tcattccccg gagggacacc agcaacgagc actgcacaaa
      901 atcctgtgat cacttacgca acacccggta cctattccgt tacgcttact gtaaccaatg
      961 gaaatggaag caattctacg accagcacca atctgataac cgtcaccacc tgtacctcac
     1021 caccaacagc ggcattctct gccagtttaa caacaatctg ttcaggagat gcggttaatt
     1081 ttacggatct gtcaaccggc tcgcctacaa gctggagctg gacatttacc ggcggaacac
     1141 ctgcaacaag tacggtgcaa aatccttcgg ttacctatgc aacgccggga acatatactg
     1201 ttacacttac cgtaaccaac gccaatggaa gtgataccga aaccattacc aatatgatta
     1261 cggtgaacaa ttgttcaacg ccacctacag ctgccttttc tgcaaataca actgcaattt
     1321 gtatcggaca acaagtaacg tttacagatc agtccaccgg cactcctacc ggttggagtt
     1381 ggtccttccc cggaggtacg cctgcaacga gcacattgca gaatccggtg attacctatt
     1441 ccactcccgg tacctatgat gtaacgctta cagttacaaa ttccggtgga agtaattcaa
     1501 caaccacaac caatctcatt gttgtttcca attgcaccgt tccggttgct tcttttacac
     1561 cttctgcaac caacctgtgc gcaggagcat gtgttacctt caataacacc agtacagatg
     1621 caacactcta caactggaca ttccccggag gaacaccatc gagttcgact cttcagaatc
     1681 ccggtacgat atgtttcaat acaccgggtt cataccaggt tgtgcttacg gtgagtgatg
     1741 gaataacatc cagttccaca acgtcaacga taactgtaaa tccgaatcca accgtttttg
     1801 ccaatgcaga tacaaccatc gatattggta actccgccat tttaactgct gtcgcaaccg
     1861 gattgggtac ctatatctgg acaccctcaa caggattgtc atcgcccggt tcagcaacaa
     1921 caactgctgc tcccttaaca actacaacct ataccgtgac cttcaccgaa aatggatgtt
     1981 cggcaaccga tcaggtcact gtttttgtta atgtgataga aggaatcggc gttcccaatg
     2041 ctttttctcc caataacgat ggaataaacg atgtgctgat ggtgtatggt cagggaattc
     2101 agggcgtgac gtttatcgtt tacaatcgtt acggacaaaa aatgtttgag acgaacgatc
     2161 cgaatattgg gtgggatgga aaatacgatc agaagatggt gaataccggt gtcttcgtct
     2221 ggtatctgga atattcgctg gtgtcgggcg caagcggaac tctgaaagga aatgttactc
     2281 taacacgtta attccaaacc gaagaaatca tgcaaaaagt aaaaatcata ttttctgcga
     2341 tgctcctcgg atggagtgtt caggtttttg gtcagcagga tattcatttc tctcagtttt
     2401 atactgcgtc cgcctatatc aatccggcaa cagcgggaat gttcaatggc gatatccgcg
     2461 gcgtgatgaa ttaccgttcg caatggaaat cggtaagcga accttttaca acatttaccg
     2521 gatctgttga tgcccgtgtt gcaaatggca gtgaaggttt tttgagtatt ggcggcgttt
     2581 tctacaatga taaagcagga tctgcaaaat tgacatccgg tcagtacgga ctcaacattg
     2641 cgtgggcagc aaaactaaaa gaggggatgt actggacatt cggtgtacaa ccttcattgc
     2701 tgaccaaaag cattaacgga gcagatttgt attacggcaa tcaatggata ggaacagaat
     2761 tcagttctgc agttgccaat aatgaaactt ttgtgaacga taaattcatg gcctttgatg
     2821 taagtgcagg atcgtattgg ttgtatcgtc cccataagga aactgcttat tatctgggtt
     2881 tagcaggagc gcacttaact cgtccgaatg taagttttct tggcaccaag gataagatgc
     2941 tgatgaaatt cacttttcat ggcggagctg aatttgaact atcaaattcc aacatggcga
     3001 tccttcccaa tttccttgtg aaaatgcagg gcgtaaaccg tattatcaat tttggtagcg
     3061 atgtgaaata cattattcag aaacaatcgc attacaccgg atattccaat gagatttctg
     3121 caggattggg tgcgtattac cgcgtgggag atgcgttttg gggagcgtta cagtttaact
     3181 ggacaggctt cacccttgca ttcagttatg atattaatct gtcaaatcta tccgttgcca
     3241 ccaatggaaa tggcggaatg gaaatgatgc tgatgtaccg tgcaggaatt ggtgccggaa
     3301 aagggaaatc gacccgattg ttataaagaa aaaatcccgc agatcgcggg attttttttt
     3361 gattctattt tccgaagtgc atccgtattc cgaattgaaa ttcatacatc attatcggcg
     3421 aacgaaaaac gtaggcgatg tgatgttgat gtctttcctg agtaaccgtg ttgacgaaat
     3481 caacattgaa aggagattta tcagcagagg tgccatgatg acctgcatgt gtttgcagat
     3541 tctgatcgtc catatccaca ctcatgtatt ccacttttcc tccggtgcga aatcctcctg
     3601 agaagaaaac ggaaaaatcc atggcgcaat cggaatgctc gcctttattg atgagtgaag
     3661 tcaaggagat ttccactcct gcaccggcat aggtggtcca ggtattgtcc tttttcagga
     3721 aatgagtttc caatgcctcg caattggttt cgtcttccgg atcccagatg ttgagtgagg
     3781 tttggaaaaa ggtaacgcca ccggtgactt cagcataggg acgaacggcg cttgcatagg
     3841 gtctgaaatc gtatttgaaa cgcatattcg gacttaagta ataattgctg atggtcatat
     3901 ccgcaataaa acgcgagcca tcttgtgtgg caaaatcgat cttggagttt ttgtttccgt
     3961 aattgccgta tgcaaaggaa aaaccaagct gtagtcgcga tccttttaag ggaccataag
     4021 catggatgtc gaatccattt actccatcga gttgcggacg cattgccgac agcgggtaat
     4081 tgttcagata actaaagcta tagctgaata aaggtttttc atagtgagct tctgcgttaa
     4141 acgaaaacag agccacgaac aatagggtag atagtttttt catacaaccg gttttgcttt
     4201 aaaaaggtag caaaaattat tcctgaatga acaccagttt aacggtttta acaaatgttg
     4261 ggaaaaacag tctgcataaa tagactccac tgctcagttc aggaacatga attgaaacac
     4321 cgttttgaat ggaatacccg gatggcgtgc tgtacaagat ctgcttgccc tggatatcaa
     4381 tcagctcaat tccgcattca agttgttgag gtatatccca ttgtatatat aaagtttgat
     4441 tgtttaggat tattgttcca ctttcagcaa ttgcatcatt tgtacccgtg ggcgcaaaaa
     4501 catacacagt tgccattgct gtgtcggaag gacaaacgct gttttctgca aggagcaaca
     4561 cctgataaat tccggactga ttataaagat gccatggagc cggatcggtt gaaaaagtgc
     4621 tgtctccaaa attccaggag taggatgatg cattctgact ccaattgctg attgccagat
     4681 acgcacccgg taggtaaagc aaggtgtcgt ttacagaaaa cgatgctacc ggaggatcga
     4741 tcaaggttat tgtaagttgt tgtgaaagta catctgtacc actcggacca tttgctgtta
     4801 gtgtgactgt ataatttcca ctcgaaggaa acaaaatttc cggatgagta gctgttgaag
     4861 tagatgggtt tccgccagga atattccaca gaaaaccggt agcatttgta gatgagttga
     4921 agatcaaaaa tggaaatgct gtgcaggcat tcattgcccc cgaagtgaaa ttagcaatcg
     4981 gagctgctgt tggttgtgta acgtaaaaac catttgagct gatcagcaat gaacgtaatg
     5041 atgcaccatt tactgctctg atgttgaggt agtaggattg accaaaaaca agatttattc
     5101 cagtgatggt atcgcctgta acattccctg tgttttgcca gtttaaaata ctactgtcgc
     5161 cgggtgttgt tccgatgctg atggaatagg tttgaattcc cgaatgtgga tcgtttgaac
     5221 ttccccaact ggcagatcgg tttaagtttt catgggtggt atcaatatca actgaaattc
     5281 cttcctgtaa ataggccggt gttgttggtg gtgtccagtc aatgttttga tcctgactcg
     5341 cactgttgct ccaattgttg gcgttgtcga tgatcattgt gcggattcgg caagaagaat
     5401 ttgcaggtcc gttgttttga taccttacca ttgcggatga tgagcccagc gaaacgtttt
     5461 ctgttgttgt tcggctgcgg tatactttta catcatcgta ttccatattc acgtttgctg
     5521 aacgcaatga aatactgttt cctgcaatta gcggagtact gtctgtccat tgggaaacca
     5581 gaacattgtt tacataagct ttaattgtgc cgctagtggg atcgtaagtg attttataat
     5641 cgtaccaggt atttgcagca atggatatag agtgatccgt cataagcgta aaaacatcat
     5701 tcaccacttc gtaaatctga catttgtttt gatcgactct gaaataaacg aaataggaat
     5761 tgccgcggtt gggtaatgat ggattgtcgc agaaaaaatg gatgccgctg cgtcgattcg
     5821 ttcctgttcc gctcattttt ccctgccagt gatagaggta ttgataggaa gatgtttgtg
     5881 taaccgccgc aaaaatattt gtattgccat tgctttcatc cgtgtgtgcc aaacgtcctg
     5941 aattaatgat ccaggtgccg ctttgttgtg tccagtcgga gtgaatggtg ccggagaaat
     6001 tatcattgaa aaaaccatga cttccattcg cacgccattc agtaccgttg ttgtccatta
     6061 cctgccaaaa ggaacgagct actccactgc cggcatcctg atcggtaaaa cttattgaga
     6121 agttggcggt tttccagttg ttgcttgcgt ttattcctgt ggttgggggt tgattgtcgc
     6181 tggtgcactg ataggtggct acaaaccccg ctgaagtggt tgaattgtcg ctggtaaaac
     6241 gcagcgttat tgctcctgtt gttcctgtga gtgccggagg tagcgttgtt cctgtaaatg
     6301 gtgatcccgg aaattgcggt gatgtggtac cgggaccatt atacacatac agaaaatcat
     6361 aattgttctc caatgaaaaa gaggagaaag ttaaagtaac agatcctgcg tttggaggag
     6421 caatggtgta ggtgtagttt tcgttgtcgt aatagttttt aaaccgcccg cctcctatat
     6481 cggttagtgt atctgaacag ggaatggtgc tgcaatcggt aaagttatcg ctgatcgcat
     6541 cccagaaatc gttatagcca tcatcatatc ccaatgccca aattcccatt ccaccaattc
     6601 ctcttttgtt gatgagatcc attctgtcac gcatatcatc tgcttcggta ataaagcatt
     6661 gtcgcaatcc accactattg aaattgtaat aggtgctttt acttgctggt tcgtaacgtc
     6721 ggttggctgc tgagaaaatt cccggaacat tgtcgcggac acttctatac gttcttgata
     6781 gtccgcttcc tgttgtagtt gctggaaggg tatgcgaact taccggccat tctcttccgt
     6841 aatagggtaa tccaagtatc agttttgcag ggggaattcc tttgttcaga taataggtga
     6901 tgcttttgga taaggtgtaa ttgtagcttg ttccgaattg aaacaatgga tcgtttggtc
     6961 ctgccgttgt acttcctgtc caataataat catagcccat tataataaaa tgatctactg
     7021 cagtcatgtt tgctacatcc agtacattat tccaatcaac agcataaagc acggtgctga
     7081 cttctgatcc cggaatggca ctgtgcatct gattggcaag atcgttcatg aaatttcgga
     7141 aattgaggga ttgcgatgca ggtaatcctt caaaatcgat gtttactcca tgggcgcctc
     7201 ttgctgaaat gagattaatc aggttggtga tgagcgtttg ttttgatgta ttgttgttta
     7261 aaaaggtagc gtgatctgaa aagagcgtaa cacaaagcgt tacctttaag ccgttgttca
     7321 acgcatcggt gaccgattgt gttgttgcaa aattataagt gttgtttgcg tttccgttca
     7381 cataatttac ttcgtaggag aaataactga aatgcgtgag caaatcccaa tcgtaattgg
     7441 attgtaaacc attgctccaa tacgggtgcc atccgtaaat tattttattg ggtgtacaat
     7501 tgtttcgcaa aacccttgtt tttggagaag gactattaat ggaataataa tcttcagcag
     7561 agtgtcctgc tgttgcatag gtttcacttt gttcctgatg aatgcttttg tgttgcgcaa
     7621 aaatggggaa tgccactaca cagaaaaaaa atgctgctgc ttttttcatc gtacaggatt
     7681 cacgaatc
//
LOCUS       DAOMYG010000102         7672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3181_length_7672_cov_9.420244, whole genome shotgun sequence.
ACCESSION   DAOMYG010000102 DAOMYG010000000
VERSION     DAOMYG010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7672
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3181_length_7672_cov_9.420244"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PK610_04660"
     CDS             complement(<1..397)
                     /locus_tag="PK610_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35253.1"
                     /translation="MITTDNYFDYIKKINISSLPENLKKGHEFVDKATRGGSSWEAYK
                     SDKIREVMDTYFKKLYEHTQEKKVSRPESSKAKKETVQKEKKTIHKEKPQPVESTKRQ
                     VEHFPEEFKFIRRFVNMNGKTKTADQIWNF"
     gene            complement(416..5284)
                     /locus_tag="PK610_04665"
     CDS             complement(416..5284)
                     /locus_tag="PK610_04665"
                     /inference="COORDINATES: protein motif:HMM:NF025240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="strawberry notch C-terminal domain-containing
                     protein"
                     /protein_id="HRJ35254.1"
                     /translation="MEKVALEFDIAEKNTVKELTELAIVMRAREFSLRTHFSIEEKFK
                     NILELYESQVNLSHRTSQSILLQQYSTPTPLSFLAGIFCEINKDTVTVFEPSAGNGLL
                     TIAADPKNVVVNEVDSLRRGNLKLQGYKQVLKQDATKPFIGFEKKFDAVLTNPPFGTL
                     DHDIRFDTFPIRTLDHLMALRALDCMKDNGKAAIIIGGHTKWDDKGRIQAGRNRIFFN
                     YLYSHYNVVDVIPIDGHKLYSRQGTAFDTRLILIHGRKSTVSGVAPVKNPDRDTPVRS
                     YEELYARIMEDMESNYLPLIQNYRSLKKKYPDAALLILKDELFYTFEEDAVRVSTILN
                     TELVKVSDGAVMIPKTEFPSESIELMMARLVRNGVRVGLVEELKASQPKKPMTDLERE
                     AEYLKSLLDDQLGAPYQPASQSCVVLNTQVPDSMSFEIQSALERIKQEVGGDIDAFVR
                     HRLGYASKVELCKALSAEQIDAVAMAIYNIEAYNEGMIIGDQTGIGKGRIAASIVRYA
                     VKQGLRPVFLTEKANLFSDLYRDLIAIGSAHLIPFIVNARESKTDIKDEDGEVIHTAL
                     SPSEQKTIFESEKVPARYDFEMATYSQFNSPEKKPEKPNFLMKQSDGNIFILDEAHNS
                     SGSSNTGEFLQRVVSHTKGVVFLSATFAKRPDNMPIYAMKTAISEANMSRDELVDAIN
                     QGGVALQEVMASQLVAEGQMIRRERSFEGVEVNYIALTDHETEHRAIADNITEIVRDI
                     IAFQTSHVDKQVEELDKIAVTEGKEVELREGTSQAGVDNQPYFSKVFQVINQMLFSIK
                     AEAVADRAIQRLKEGKKPVIAFASTMGSFIEDMENEKGLPVGDGDLVNADFAEVLRKG
                     LDGILRYTEKDVNGDAIHKKFAVSELPVDAQMEYNRIWEKIKKASTGITISPIDVIIR
                     KLTQAGYSVAEVTGRKYEMQINPKTNMGLVVTRKRINTNDAFRQFNNNEVDVLMINQS
                     GSTGASAHAIPTSKVPASEVRQRVMIVLQAELDINTEVQKRGRINRTGQILKPIYDYV
                     NSAIPAEMRLMMMLQKKLKSLDANTTSNQKQSTKILDVPDFLNKYGDKVVKEYLLENP
                     QVNDLIGDPLHLKKSGSDASSQDESRGDNESIEDAAHRVSGRVAVLSTAMQQDFYNEI
                     ADRYSDYVEYLKQISEYDLEVETMNLQTETISTKIIKAGKGGRSAFGDDSILETVRAN
                     VLKKPFSKVELENILRESLQGLKPEELKQDTIHEFESWFENKLKEDLLENENKYNLLI
                     VNIPHEKKLLKIKEKQGDKAYQAAIKEREEELSKARMAAQVNIEKTYENRRTYLLRMF
                     KFFHVGRKVNYPVESFEQGQQLIPSVFLGFVIDKKKKNPFAPSSVKLRFAISNSSKYI
                     AIPASYSKDISSIIGASVDVSQPDEETLLELWEQLIKESTTDRKIRHIITGNLLQAFS
                     DFRGKLVSYTTLSGDTKKGILMPENWSPGEAVQNKVVVPIYKALPLIKSLSNGAAIYA
                     GAVALFRHGEGYKMLVPSSRSKGGDIYLDSDLLPLVEKNNFEKTSDKMVAFIPFKNIE
                     PVVKILQEKFGLSVTVSESNFEMIQQNSGRMDTKKKPIVLPPAEETITIDLNMLELEA
                     EALLLMLELEAA"
     gene            complement(5375..6010)
                     /locus_tag="PK610_04670"
     CDS             complement(5375..6010)
                     /locus_tag="PK610_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35255.1"
                     /translation="MKNWNASRIVLWITVVQFFFTLTFYWFSFQKIAGNNPVYDKGAT
                     ETIESDVKQINKDYSISESNYKKAIDSLNTKLFYTDLLLKKEMFISKERKNDLENILS
                     QRWDTLSTEIRLSECDSLRERTKEFIIAQEIKDTLFQNKIAQLTALHAESQKQLFTCD
                     TTGRRLNDQINAALIHTKQCEELMKKQKRQNRILKIGMGIIAVITVGLAVK"
     gene            complement(6007..6399)
                     /locus_tag="PK610_04675"
     CDS             complement(6007..6399)
                     /locus_tag="PK610_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35256.1"
                     /translation="MNQKQEQKVKRSPIIKEIFKDKNGFFNMRELTTALMVIMVTVGW
                     VGQQFFGFILPDFMFFGFISLIASGCFGYSIEKKQRHLDKQESPTVMNIISEVVQGFT
                     QANRKKPQASKEPEETNVEFEKEEEEEK"
     gene            complement(6353..7165)
                     /locus_tag="PK610_04680"
     CDS             complement(6353..7165)
                     /locus_tag="PK610_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010181381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35257.1"
                     /translation="MAEKLHPIGSERKSRSGGGRGVMGVLGKVKTVLRMKGFEIYDRP
                     YELNIVGIRSHHSKANRFDDEMHVFFKTNSGGWEHYIFKITTDPGTYWLHNPMMPQGT
                     AILKEGQYKNVYQIGLHRGKYYALIQGGGTVTVIRDYNRDAILDWYNGKEQKGWFGIN
                     IHRAMQKGTAKYVEEFSAGCQVFENAADFDLFMTLAQRHRSLYGNKFTYTLIDYRAMK
                     RASLRRWVTGIGIGAVALALFFPAVKEILSEILNDETNESETRTKSETVSNH"
     gene            complement(7099..7563)
                     /locus_tag="PK610_04685"
     CDS             complement(7099..7563)
                     /locus_tag="PK610_04685"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRJ35258.1"
                     /translation="MSRNVKYIVLHCTDTSPLASMEAIKRYWKEHNKWERPGYHYIIK
                     SNGEVVKLLNENLVSNGVLGFNQQCINIAYIGGKTKEGKHADTRTPQQEDAMYELIMW
                     IKERHPNAELKGHCEFPNQGGRTCPNFDVKEWQKNYIPSVVRENPDQEEDEE"
BASE COUNT         1925 a         1664 c         1440 g         2643 t
ORIGIN      
        1 gaaaattcca aatctgatct gcggttttgg ttttaccatt catgttcaca aacctgcgaa
       61 taaacttaaa ttcttcagga aaatgttcca cctgtctttt agtggattca acaggttgcg
      121 gtttttcctt atgaattgtt ttcttttcct tctgaactgt ttcctttttt gctttagatg
      181 attcaggacg tgaaactttt ttttcttgcg tatgctcata aagtttttta aagtacgtat
      241 ccattacctc acgtatttta tcgctcttat atgcctccca tgaagagcct cctcttgttg
      301 ccttatcaac gaactcatgc ccttttttca agttttcagg taaagacgaa atgtttattt
      361 ttttgatgta atcgaaataa ttgtcagtgg taatcatggt aaaatatttt ttaggttagg
      421 ctgcttcgag ttcaagcatt agtaaaagtg cttcggcttc cagttcgagc atgttcaggt
      481 caatggtgat ggtttcctct gccggtggta atacaatcgg tttctttttg gtgtccattc
      541 gtccgctgtt ttgctgaatc atttcaaagt tggattcgga aacggtaacc gataaaccga
      601 atttctcctg gagaattttt accacaggtt caatattctt aaacgggata aaggcaacca
      661 ttttatccga agtcttttca aaattgtttt tctccaccaa tggaagtaaa tccgaatcga
      721 ggtaaatatc tcctccctta cttcgggatg aggggacaag cattttatac ccctcaccgt
      781 ggcgaaaaag agcaactgct ccggcataaa tggctgcacc gttagaaaga gatttgatta
      841 atggcaatgc tttgtatatc ggaactacta ctttattctg taccgcctcg cctggagacc
      901 agttttccgg catcaggatt cccttcttag tgtcaccgga aagtgtggta taactcacta
      961 actttcctct gaaatcagaa aatgcctgta ataagtttcc ggtgatgatg tgacggattt
     1021 ttctatcggt ggtgctttct ttaatgagtt gctcccatag ttccaataaa gtttcttcgt
     1081 ccggctgtga cacgtccaca cttgctccaa tgatggagga tatatctttt gaatacgaag
     1141 ccggaatagc aatgtattta ctactgttag aaatggcaaa gcgtagtttt acagaacttg
     1201 gtgcaaaggg attctttttc tttttgtcaa tgacaaatcc taaaaacaca cttggtatta
     1261 attgctgtcc ctgttcaaag gattccaccg gataatttac ttttcttccc acatggaaaa
     1321 atttgaacat gcgaagtaaa tacgttcttc tattttcata agttttttca atgttaacct
     1381 gcgcagccat tcttgctttg cttaattctt cttcacgctc tttgatagct gcctgatacg
     1441 ctttgtcacc ttgcttctct tttatcttta aaagtttttt ctcatgggga atatttacaa
     1501 taagtaaatt gtatttgttt tcattctcca ataaatcttc cttgagtttg ttttcaaacc
     1561 aagattcaaa ttcatggatg gtatcttgtt tgagttcttc cggttttagt ccttgcaaag
     1621 attcacgcag tatattttca agttccactt ttgaaaacgg ttttttcaaa acattggcgc
     1681 gaactgtttc taaaatacta tcatcaccaa atgcacttcg tccacctttt ccggctttga
     1741 ttattttggt agaaatggtt tccgtttgca ggttcatggt ttctacttcc aaatcatatt
     1801 cgctgatttg tttgaggtat tccacataat cactataacg gtcggcaatc tcattgtaaa
     1861 aatcctgttg cattgccgtt gatagaacag ctacgcgacc agaaacacgg tgagccgcat
     1921 cttcgatact ttcattatca ccacgacttt catcctgaga ggaggcatcc gaaccgcttt
     1981 ttttcaagtg aagcggatca ccgatcaaat cgtttacctg tggattctcc aatagatatt
     2041 ccttaaccac tttatcaccg tacttattta aaaaatccgg cacatctaaa atcttggtgc
     2101 tttgcttttg gttggaagtg gtattggcat ccaaagattt taatttcttt tgtagcatca
     2161 tcatcagtcg catttctgcc ggaattgccg agttcacgta atcgtaaatc ggttttagga
     2221 tttgcccggt gcggtttata cgaccacgtt tttgcacttc ggtatttata tccagttccg
     2281 cttgcaatac gatcattacc cgttgcctta cttcacttgc cggaactttg cttgttggaa
     2341 tggcatgtgc cgatgcaccc gtacttcctg attgattaat catcagaacg tccacctcgt
     2401 tgttgttgaa ctggcgaaaa gcatcgttgg tgtttatccg ttttcttgtc acaaccaatc
     2461 ccatattggt tttaggattg atctgcattt catacttacg tccggtaact tctgctaccg
     2521 aatatccggc ttgtgtgagt ttacgaatga taacatcaat aggagaaatg gtaattccgg
     2581 tggatgcttt tttgatcttt tcccaaatgc ggttgtattc catttgtgca tcaacaggaa
     2641 gttccgatac cgcaaacttt ttatgaatgg catctccgtt tacatctttt tcggtgtagc
     2701 gtaaaattcc gtccagtcct tttctgagta cttctgcaaa atctgcattg actaaatctc
     2761 cgtccccaac aggtaaacct ttttcgtttt ccatgtcctc aataaaactt cccattgtag
     2821 aggcaaaagc aatcaccggt tttttacctt ctttcaatcg ttgtatggct cgatccgcca
     2881 ccgcctcggc tttgatgcta aagagcattt gattgatgac ctggaagact tttgagaagt
     2941 agggttgatt atccactcct gcctgtgaag ttccttcacg cagttccact tcttttcctt
     3001 cggtaacggc aattttatcg agttcctcca cctgtttatc tacatgcgaa gtttgaaagg
     3061 cgataatatc ccgaacgatt tccgttatgt tatcggcaat ggcgcgatgt tcggtttcgt
     3121 gatcggttaa ggcaatataa tttacttcta caccttcaaa ggaacgttcc ctgcgtatca
     3181 tttgcccttc ggccacaagc tgtgaagcca ttacttcttg caaagcaaca ccgccctggt
     3241 taatggcatc tactaattca tccctgctca tgttcgcttc tgaaattgcg gttttcattg
     3301 cgtaaatcgg catgttatcc ggtcgctttg caaaagttgc cgatagaaat accactcctt
     3361 tggtatgaga aaccacacgc tgcaaaaatt ctcctgtatt ggatgagcca cttgaattat
     3421 gtgcttcgtc aaggataaaa atatttccat cggactgttt cattaagaag ttgggcttct
     3481 ccggtttttt ctctggtgaa ttaaactgcg aataagttgc catttcaaaa tcgtaacgtg
     3541 caggaacttt ttcactttca aaaatggttt tttgttcgga aggtgaaaga gccgtatgta
     3601 tcacttcacc atcttcatct ttaatatccg ttttcgattc acgagcattt acgataaacg
     3661 gaattaaatg tgcagagcca attgctatca agtcacggta caaatcggaa aacaagtttg
     3721 ctttctctgt aagaaaaacc ggacgaagac cttgcttgac tgcgtatcgc acaatagaag
     3781 ctgcaatacg tccttttccg attcctgttt gatctccaat aatcattccc tcgttatagg
     3841 cttcgatatt gtagatcgcc attgcaacgg catcaatctg ttcggcagaa agtgccttgc
     3901 ataattctac tttactcgca taacccaatc ggtgacgaac aaaggcatca atgtctccac
     3961 cgacctcttg ttttattctc tctaatgcag actgaatttc aaaactcatc gaatcgggaa
     4021 cctgtgtatt caacactaca caactttgtg aagccggttg ataaggtgcg cctaattggt
     4081 catccaataa tgatttcaaa tattctgctt cacgttccaa atcggtcatt ggttttttcg
     4141 gctgtgatgc tttaagctcc tcgactaaac caacgcgcac accgttacgc accaatctcg
     4201 ccatcattag ttcgatggat tcagagggaa attccgtttt aggaatcatt accgctccgt
     4261 ccgatacttt aacaagttct gtatttaata tcgtagaaac acgaactgcg tcttcttcaa
     4321 acgtgtaaaa caattcgtct ttcagaatta gtaaagcggc atcgggatat tttttcttta
     4381 atgaacggta attttgtatt agtggtaaat agttactttc catgtcttcc attattcttg
     4441 cgtacaattc ttcgtatgat cttactggcg tatcacggtc gggatttttt actggagcaa
     4501 ctccacttac tgtgcttttt cttccgtgaa ttaggattaa gcgcgtatca aaggctgttc
     4561 cctggcgtga atagagttta tgaccgtcta ttggaattac atcgaccacg ttgtaatgcg
     4621 aataaaggta attgaaaaaa atccggtttc ttccggcttg tattcttccc ttatcgtccc
     4681 atttggtatg accacctatg ataatggcag cttttccatt atctttcata caatccaggg
     4741 cgcgtaatgc cattaagtga tcgagtgtac gaatgggaaa tgtatcgaaa cgaatatcat
     4801 ggtcgagtgt gccgaatggt ggattggtta agactgcatc aaactttttt tcaaatccga
     4861 taaatggttt tgtggcatct tgttttaaaa cctgtttgta gccttgcaat tttaaatttc
     4921 ccctgcgaag tgaatccact tcattcacca ccacattttt tggatcggct gcaattgtga
     4981 gcagaccgtt acctgcgctt ggttcaaaca cagtcacggt gtctttattt atttcgcaga
     5041 aaatacctgc aagaaaactc aagggtgtag gtgtggaata ttgctgcaat aaaatacttt
     5101 gcgaagtgcg gtgcgaaaga ttgacctgac tttcgtacag ttctaaaatg ttcttgaatt
     5161 tttcttcaat agaaaaatgc gtgcgcagag aaaattccct ggcacgcatt acaatggcaa
     5221 gttccgtgag ttctttaacc gtattctttt ccgctatatc aaattcgagc gcaacctttt
     5281 ccacgcttcg ttttgtatgc ttagaaccga tctgtaactc acggagcatc cttcttacaa
     5341 acaagttata attctgctcg tatgccatct tattttactt aactgctaat ccaactgtga
     5401 taactgcaat aattcccatc ccgattttta aaatccggtt ttgccttttc tgtttcttca
     5461 tcaactcctc gcattgcttt gtatgtatca aagccgcatt gatctgatcg tttaaccgcc
     5521 tacctgtcgt atcacaggta aacaattgtt tttgcgactc agcatgaagg gcggttaatt
     5581 gtgctatctt attctgaaaa agggtgtctt ttatttcctg ggcgataata aactctttgg
     5641 ttcgctcccg taatgaatca cactcagaaa gtcttatttc tgttgaaagt gtgtcccatc
     5701 gttgtgaaag aatgttttcc aaatcatttt ttcgctcttt ggaaataaac atttcctttt
     5761 tcaataacag gtcggtgtaa aacaattttg tattaagact atcaatggct tttttgtagt
     5821 ttgattcgct tattgaatag tccttattga tttgctttac atcactttca attgtctcag
     5881 tcgctccttt atcataaacc ggattgttcc ctgctatttt ttggaaacta aaccagtaaa
     5941 aggtgagtgt aaaaaagaat tgcaccacag ttatccataa tacaatccgg cttgcattcc
     6001 agtttttcat ttttcttctt cctcttcttt ttcaaattca acattggttt cttccggctc
     6061 tttactggct tgtggcttct tgcggtttgc ctgtgtgaac ccttgcacca cttcactgat
     6121 gatattcatc accgtgggcg attcctgctt gtctaggtgt cgctgctttt tctcaatgga
     6181 ataaccaaag caaccggagg cgatcaggga gataaaccca aaaaacataa agtcaggtag
     6241 gataaatccg aaaaactgct gacctaccca tcctacggtg accattatca ccatgagtgc
     6301 ggtggttagt tcccgcatat tgaaaaatcc gtttttgtct ttgaagatct ccttaatgat
     6361 tggagaccgt ttcacttttt gttcttgttt ctgattcatt tgtttcatcg tttaaaattt
     6421 ctgataagat ttctttgact gccggaaaga atagtgcaag ggcaaccgca ccaattccga
     6481 ttcctgttac ccatcgtctg agtgaggcgc gtttcattgc gcgatagtca atcaacgtat
     6541 aggtaaattt gttgccatat aacgatctat gtctttgcgc aagggtcata aacagatcga
     6601 agtcggctgc gttttcaaat acctggcatc cggcagaaaa ttcttccacg tatttggcgg
     6661 ttcctttttg catggcgcgg tgaatgttta ttccgaacca tcccttttgt tccttcccat
     6721 tgtaccaatc caaaatagca tcgcggttat aatcccgtat cacagttacc gttccaccgc
     6781 cttgtatgag cgcgtaatat tttcctctgt gcaatccgat ctgatacacg ttcttgtatt
     6841 gtccttcctt gaggatggct gtgccttgag gcatcattgg attatgcagc cagtaggttc
     6901 ctgggtcggt agtgatcttg aaaatgtaat gctcccaacc accgctgttg gttttgaaaa
     6961 aaacgtgcat ctcatcatcg aagcgattcg ctttagaatg atgcgaacgt attccgacaa
     7021 tgttcagctc atagggtctg tcgtaaatct caaaaccctt catgcgcaaa accgttttta
     7081 cttttccaag aacacccatt actcctcgtc ctcctcctga tcgggatttt ctctcactac
     7141 cgatgggatg taatttttct gccactcctt cacatcgaag ttgggacagg tacgacctcc
     7201 ctgatttgga aactcacaat gccctttgag ttctgcatta gggtgacgtt ctttaatcca
     7261 cataatgagt tcgtacatgg catcctcctg ttgtggagtt cgtgtatcag catgttttcc
     7321 ttctttggtt tttccaccga tataggcaat gttgatacat tgctgattga atcctaatac
     7381 cccatttgat accaaatttt cattgagcag tttaactact tcgccattgg atttaatgat
     7441 gtagtgataa cctgggcgtt cccatttgtt gtgttccttc cagtaccgct tgatagcttc
     7501 catagaagca agcggagaag tgtccgtgca atgaagcacg atgtatttta cgtttcttga
     7561 catttttttt gattagttgt tttgaataat tctcactgat ttttctttta caatcaatct
     7621 tcgcttaccg tccattgttt gaaaaagtgt gaatcctttt ttggtttcta tt
//
LOCUS       DAOMYG010000103         7639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3206_length_7639_cov_11.005670, whole genome shotgun sequence.
ACCESSION   DAOMYG010000103 DAOMYG010000000
VERSION     DAOMYG010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7639
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3206_length_7639_cov_11.005670"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            527..1729
                     /locus_tag="PK610_04690"
     CDS             527..1729
                     /locus_tag="PK610_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ35259.1"
                     /translation="MNKGWIIITLCGFGLSLQAQNLVSNPSFEIKSECPVENGKLEKA
                     VGWINPSKATPDLFCHCAQNKLAPVSTPQNGMGYQDPYSGSCYAGLFVYTVNDTLYGE
                     YIQTKLDHPLVKGQTYHVSFWYSLADHSMYKAVSIGIVLTQEKLKKKGNERFHELPSI
                     VNDEPMSNDTLQWQLFHSSYIAKGGEEWLTIGPFGEYRYDEKMRKKIKVPARYKYMIP
                     GEAAYFYIDEVYVSDKPCPFDSTRAVAKTKHSHQHPHKEIVKKDTLRKNEKKNEIVSS
                     NVISETKTEVKKEEPVIFKELVFESGKSVILPASYAELDRMVSLMKTKPEMKIMITGH
                     TDNTGNEKSNLELSRQRAKAVADYLISKGIDSKRINHEGKGSAEPRAENSSEEGKAKN
                     RRVEFILK"
     gene            complement(1736..1972)
                     /locus_tag="PK610_04695"
     CDS             complement(1736..1972)
                     /locus_tag="PK610_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HRJ35260.1"
                     /translation="MEDPGIPDNKQLLALVSTRMPYGKYKDWYLYRLPVSYLEWFARK
                     GFPKGKLGDLLQLMYVIKLNGLEDLLKPLITITK"
     gene            2171..5404
                     /locus_tag="PK610_04700"
     CDS             2171..5404
                     /locus_tag="PK610_04700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ35261.1"
                     /translation="MRVFFQRLSFLLLLVFAIGPNLIAQTLIINEVSNGPTGNKEYVE
                     LVVIDTTIVYDCNATTPPCIDIRGWIFDDNSGYHGAGGVASGAIRFANHPLWACVPVG
                     TIILIYNNGDPNTSVPPNDLSTTDGNCVIIAPVASIYFDRNATTPGAAACSYPAAGWI
                     NGGDWTMTALANSGDCARIVDLSGCEVFSLCYGATNSNTLIYFNSGGSGAQNVWWFNS
                     GNPYTTGSWSEGCASIATCGSDEQTPGAPNNAANAAYIAQFNNNCLPIPPLTTNLSNS
                     SNAVCGCAGSATVAVSGSIAPYTIIWTNSSFSPIGQTGLLATNLCAGTYHAIINSSIN
                     CADTITVTIAASSAINPQLNFSPVSCAGSANGQASVNPIGGTGPYTILWSPAPGSGQG
                     TTTATGLTPGNYSVQITDNTGCTQSASFTITQPSALNAVLSPVNPTCNGACDGSITSA
                     VSGGTGSINLIWSNGTNGANINGLCAGNYSVTLTDQNNCTATFSSQLTDPAAIDVIIS
                     HTDAGCGGVPGTATAIANGGTGAFSYIWFDSQFNVYPSTPTISNLLPDDYTVVVSDAN
                     GCSVAGMVTVTQGFPPSLSETHTDLLCNGNLSGTIDLSISGIAPFQIAWTGPLGFTSS
                     QEDIGNLSAGVYTAIVNDASGCNAILSITLTEPNALVVSLNNPGTVCSSSSTTITTNV
                     SGGTSPYSYNWGGSHTSSSLTFQTVSDTLVCVAVSDANNCPVVNECRSVSVFPSLVYN
                     GPADVTLCEGSSTNLSLSVSGGTGAPYGITWSDGSSIISTTNALSVNPTGTISYFISV
                     QDACAANAVFDTVEVQVIAVPVPAIAAINTSGCAPLTVQFSESNAVAGLNCVWTFGTA
                     GSNDDCGATSFTFTQAGCYDISYSLITPSGCTSSVTAQQLVCVFPDPVASFTPSSQQV
                     SSYNNVVTMNNSSFGASDYLWTFNGLPIGTDLNPQITFPSEEAAVFEVCLNATSNLGC
                     VDSTCRFIESIPDLAVYVPNAFTPNHDPINNSFSPVISGYPNEYLFRIFNRWGELIFE
                     SKTPGESWDGNYLGVPCQVDTYVWYLMVYVENPGKEYRYRGHVTLFR"
     gene            complement(5508..5936)
                     /locus_tag="PK610_04705"
     CDS             complement(5508..5936)
                     /locus_tag="PK610_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35262.1"
                     /translation="MDHKTLGIQIGFDEERPFGVIMDWYVGNGVATLACFSTGDASLY
                     FSGGTTMIGGIGIPAVKELAPQYVMNAAKVIPLCSKVNAFPAPDPQTVKFYILTNKGK
                     YAGSESMAQFQTGQSSWFPLFIDANTIMAELRKKHEGQPK"
     gene            6398..7522
                     /locus_tag="PK610_04710"
     CDS             6398..7522
                     /locus_tag="PK610_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ35263.1"
                     /translation="MQSPFVYGRIAEGSAFANRKQELQRLSGNLRNKINTIVLGPRRI
                     GKTSLVRKITAEYSGLSYYRFCYIDAYKVNDEESFYSYFASEVIKACSSNVEEAIEYS
                     RQLMAKSSSRLAVSLTQSRDYNLAVVTEDEIGDDAMDFGNEIARKKNFTLIICIDNFQ
                     NIERFEQSLLFQKKFKTAIEKHDKTVYCIAGTRTSFLNDAFLNPDQPLYQMGDLIQLD
                     RIALDELAPLIMNRFSESGKSCAMMFAERICKEMVCHPYFTQQLAHIVYMNANGKVDD
                     AVISRSVEEMIERNLVSFQREYENLSILQANFLRMLLDGVEDGFTTKPMIEKYKLSSS
                     AAVIRVIDSLLKKEVIERRQGKFCFVDPTFHYWMQRVVKG"
BASE COUNT         2117 a         1779 c         1575 g         2168 t
ORIGIN      
        1 caatgtatac atcttcattc cataatcatc agctcctagt tttcgggcta aagtagaatc
       61 gtaagtttct gaatttgtgt tctgggcaaa aagtgataaa gaatgaacta caaaaagaag
      121 aatcaatata tgtttcattg acacaggttt tatcaaaaat agtcattcca taggttcgtc
      181 tcactatcca attgtgaccg aactactcac ttgacataaa aactggtgct atttcacttg
      241 attttctcag ttaacataaa gaactttgca gcatcgcagg cgagtagacc tccgggattt
      301 taaaaatccc ggaggtcttc gtcagggctg ccggatttga atgggtgttt cctttaatca
      361 actccggaaa aaacacatgc ccattcctta tatatccata ttcttcggct ccaacagctt
      421 tcaaatgttc cttattattt acttctaaat aagttttgtc taaaatttga cgaaaacaac
      481 aggcgggttg tttctaaata ataacctgtg cactatcttt ccgctcatga acaaaggctg
      541 gataattatt acgctatgtg gttttgggct ttcattgcag gcgcaaaatt tagtgagcaa
      601 tccgtcattt gaaatcaaaa gtgaatgccc ggttgaaaat gggaaacttg agaaagccgt
      661 tggctggatc aatcccagta aggctactcc tgatctgttt tgccattgcg ctcaaaataa
      721 actggcccct gttagcacac cgcaaaacgg aatgggctat caggatccat acagcggatc
      781 ttgttatgca ggtttgtttg tgtacacggt taacgatacg ctatacggag agtatattca
      841 aaccaaactc gatcatccct tggtgaaagg ccaaacctac catgtgtcgt tttggtattc
      901 gctggccgat cacagcatgt acaaagcggt ttccattggt attgtgctta cacaggaaaa
      961 actaaagaaa aaaggcaacg agcgctttca tgaattgcct tcgatcgtta acgatgagcc
     1021 gatgagcaac gatactttgc aatggcagtt gtttcactca agttatattg caaaaggtgg
     1081 agaagaatgg ctgaccatcg gtccttttgg agaatacagg tacgacgaaa aaatgcgtaa
     1141 aaaaattaaa gttcctgcaa ggtataaata catgattccg ggtgaggcgg cttatttcta
     1201 catcgatgaa gtatatgtaa gcgacaagcc ttgtccgttc gattctacac gtgccgtcgc
     1261 gaaaacaaaa cactcccacc aacaccctca taaggaaatt gtaaaaaaag acactctgcg
     1321 caagaatgaa aagaaaaatg aaatcgtttc ttccaatgtg atttccgaaa ctaaaacgga
     1381 agtcaaaaaa gaagaacctg taatttttaa agaacttgtt ttcgaaagcg ggaaatccgt
     1441 tattcttcct gcatcttatg ccgaactgga tcgcatggtt tcgctaatga aaacaaaacc
     1501 tgaaatgaaa attatgataa ccggacatac cgataatacc ggtaatgaaa aatccaatct
     1561 ggaactttca cgacaaagag caaaagctgt tgccgattat ttaatttcaa aaggaatcga
     1621 ttcaaaacgg attaaccacg aaggcaaggg cagtgcagag ccacgagctg aaaattccag
     1681 cgaagaagga aaagccaaaa atcgccgtgt cgagtttatc ctgaagtaag tagaattact
     1741 tcgtaattgt aattaatggc ttcagtaaat cttccagtcc gttcaatttg atgacataca
     1801 tcaattgcaa caagtcgccc agcttcccct ttggaaatcc tttgcgcgca aaccactcca
     1861 aataactaac gggcagccga tacaaatacc aatccttgta cttgccataa ggcatgcgcg
     1921 tactcaccaa ggctagcagc tgcttattgt ccgggattcc gggatcttcc atggcctaaa
     1981 gataaaaggt tctttttcgt caccttaaga ttcagtctca cctttaatac caggggtttc
     2041 agcaaaattt aacccctgtt taacctttgc tgaatgcatt tagggtattt ttgtactgcc
     2101 gcagtgaaca cccttgaggt tttctccctt acccgagatc agccgaagcc gcgcggaacg
     2161 atgtgttcgc atgagggtat tctttcaacg tttgtcattt ctgttgctct tggtctttgc
     2221 cattggccca aaccttattg cacaaacact catcatcaat gaagtttcca atggtccaac
     2281 cggcaacaaa gaatatgttg aacttgtggt tatcgataca accattgtct acgactgcaa
     2341 tgccacaacg cctccttgta tcgacatccg tggttggatt ttcgacgata atagcggcta
     2401 ccatggtgca ggtggagtag catcaggcgc catccgattt gccaatcatc ctttgtgggc
     2461 ttgtgtaccg gtggggacaa tcattctcat ttacaacaac ggcgatccca acactagcgt
     2521 tcctcccaac gatctctcta ctaccgacgg aaattgcgtt atcatcgctc cggttgcaag
     2581 catctatttc gatcgcaatg ctactacacc tggtgcagca gcctgcagtt atccggcagc
     2641 aggatggatc aacggtggcg actggaccat gaccgctttg gccaattcgg gcgactgtgc
     2701 acgtattgtc gatttaagcg gatgcgaagt tttttcattg tgctatggcg caacaaattc
     2761 caatacgctg atttatttta attccggcgg ctccggcgca caaaatgtat ggtggttcaa
     2821 ttcgggtaac ccctatacta caggtagctg gagtgaaggt tgtgcttcca tcgcaacatg
     2881 cggaagtgat gaacaaactc cgggagcgcc caacaatgca gccaatgcgg cgtatattgc
     2941 tcaattcaat aacaattgtt tacctattcc tccgcttaca actaacctca gcaactcaag
     3001 caatgctgtt tgtggatgcg cgggttcggc tactgttgca gtaagcggtt ctatcgcacc
     3061 ttacacaata atctggacaa actcctcctt ctcccctatc ggacaaacag gcctgcttgc
     3121 tactaacctc tgcgcgggaa cttatcatgc aattattaat tcttccatca attgcgccga
     3181 cactattaca gtaactattg ccgcttcatc ggcgatcaat ccgcaactta atttcagtcc
     3241 tgtaagctgt gcaggaagcg caaacggaca agcatccgta aatcccattg ggggtacagg
     3301 tccatacacc attctttggt cgccggcacc gggttccgga cagggaacga ctactgcaac
     3361 ggggttaacg cccggaaatt acagcgtgca aattaccgac aacacaggat gtacacaatc
     3421 tgcttcattt accataacac aacccagtgc actcaatgca gtactaagcc cggtcaaccc
     3481 aacttgcaat ggtgcttgcg acggtagtat tacttcagct gtttcgggag gcacaggtag
     3541 catcaattta atatggtcga acggtacgaa tggcgctaac attaacggac tgtgcgcagg
     3601 taattattct gtaacactta ccgatcaaaa caactgcact gcaacatttt cttcacagct
     3661 aaccgatcct gccgccattg atgtaatcat atcgcatacc gacgcagggt gtggtggtgt
     3721 tcccggcact gcaacagcga ttgctaatgg tggaacggga gctttttcct atatctggtt
     3781 cgactcacaa ttcaatgttt atccttcaac tcctacaata agcaacttac ttccggatga
     3841 ttacactgtt gttgtttcag atgccaatgg ttgctctgtt gcaggaatgg taacagtaac
     3901 tcaaggtttt cctccttcac tttctgaaac gcacactgat cttttatgca atggtaattt
     3961 gtcaggaaca attgatctga gtatttcagg aatcgctcct tttcaaattg cttggaccgg
     4021 gccattagga tttacttcct cgcaagaaga tattggcaac ctttctgctg gtgtgtatac
     4081 tgcaattgtt aacgatgcaa gcggatgtaa tgctatactt tcgatcacgc tcactgaacc
     4141 gaatgctctt gtagtaagcc tcaacaatcc gggaacagtt tgttcttcct cttcaaccac
     4201 tattaccaca aatgtaagcg gtggaacttc tccatattcc tacaattggg gtggctcaca
     4261 tacttcctct tcgttaactt ttcagactgt ttcagataca ctagtttgtg ttgccgtttc
     4321 agatgcaaat aattgtccgg tagtaaatga atgccgctct gtttctgttt ttccttcact
     4381 ggtttataat ggtccggcag atgttactct gtgtgaagga tcaagcacca acctttcgct
     4441 ttccgtatcg ggaggtacag gcgctcctta cggaataacc tggagtgatg gctcaagtat
     4501 tatctctaca acaaatgctc tttccgtaaa tcccaccggt accatttcct attttatttc
     4561 cgttcaggat gcctgtgcgg ctaacgctgt ttttgataca gtagaggttc aggtaatcgc
     4621 tgtgcctgtt ccggcaattg ctgcaataaa cacttccggt tgcgcgccct tgacagtgca
     4681 gttttcagaa agcaatgctg ttgcaggatt aaattgcgtg tggacattcg gcacagcagg
     4741 ttcgaacgat gactgtggag caacgtcatt tacgtttacg caagcgggtt gttacgatat
     4801 ttcttattcg ctaattacac cctccggttg tacctcatcg gtaactgcac aacaattggt
     4861 ttgtgtcttt ccggatcccg ttgcatcatt cactccctcc agtcaacagg tgtcgtcgta
     4921 taacaacgtg gttacaatga ataactcctc attcggagcg agtgattatt tgtggacatt
     4981 taacggactt cccatcggca ctgatcttaa tcctcaaatc acttttccaa gcgaagaagc
     5041 tgcagttttt gaagtttgtc tgaatgcaac aagtaatctt ggttgtgtgg atagcacctg
     5101 caggttcatt gaatccattc cagatcttgc agtgtatgtt ccaaacgcat ttacgcctaa
     5161 ccacgatccg ataaacaatt ccttctctcc tgttatttca ggttatccca acgaatacct
     5221 gtttcgcatc ttcaaccgtt ggggtgaact gattttcgaa agtaaaactc cgggggaaag
     5281 ctgggatgga aattatttgg gcgtgccttg tcaggttgat acctacgtat ggtacctgat
     5341 ggtttatgta gaaaatcctg ggaaagaata ccgataccgc ggacacgtta ccctgttccg
     5401 ataacaaaaa acttcacctg atttttactc ggaagaactg attacggacg atcaagcaac
     5461 tgaataatgc tttttgccat gttgctgcct tcattggtaa aatgttttta tttgggttga
     5521 ccttcatgtt tctttctcaa ctctgccatg atggtattgg catctatgaa cagaggaaac
     5581 caggaagatt gtccagtctg aaattgagcc atactttctg atcctgcata tttcccttta
     5641 ttggtcagga tgtaaaactt cacagtttgc ggatcgggcg caggaaatgc atttacctta
     5701 ctgcaaagtg gaatcacctt tgctgcattc atcacatatt gcggagcaag ttccttcacg
     5761 gccggaatcc cgatgcctcc gatcatggta gttccgccac tgaaatacaa acttgcatca
     5821 ccggtagaaa aacaagctaa cgttgccact ccattgccaa cataccaatc cattatcact
     5881 ccaaacggac gctcttcatc aaagccaatc tgaataccca aggtcttatg gtccatattc
     5941 aaagcctgat ttctcagatt ctcgtaaggg tttacattga ctttagcttc cggttccttt
     6001 ttgcgcttat ctcaactgtc tccgctgcca agaataatcc aggcaaaaac aacagcaata
     6061 atcactaaca gaacgatcca aacgacttgc atatactcag gattttcccc gaatatccag
     6121 ctttttcccg ctcatgaagg atccgatctg gcaggttatg aacagcactc gggaatccaa
     6181 ttgttaatcg gtttaactat tcacatcttg ttcatttcgt cgaataccca taaatttcta
     6241 gcgaacttat ttgttagtat gataaaagtt gctaggtttg ttaacacagg agtcctgaac
     6301 gattgttgat aacccaagac ttaattcgga ttaaggcttt tgggggtgaa agaaggcccg
     6361 ggcttggatt cccgaaccaa actgttaata accggatatg cagtcaccct ttgtctacgg
     6421 acgaattgcc gaaggatcgg ctttcgccaa ccgcaaacaa gaactccagc gtctgagcgg
     6481 aaacctccgc aataagatca ataccattgt gcttggtccg cgtagaattg gcaaaacatc
     6541 actggtacga aaaatcaccg ctgaatattc cgggctctct tactaccgtt tttgctatat
     6601 cgatgcctac aaggtgaacg atgaagaaag tttctacagc tattttgctt ccgaagtgat
     6661 caaggcttgt tcatccaacg tggaagaagc cattgaatac tcacgccagc tgatggcaaa
     6721 atcctcttca cgtttagctg tttctttaac gcaatcgcgc gactacaatc ttgcagtagt
     6781 taccgaagat gaaataggtg atgatgctat ggatttcggt aatgaaattg cacgaaaaaa
     6841 gaatttcaca ctcattatct gtattgataa tttccagaat attgagcgct tcgagcaaag
     6901 cctgttgttt cagaaaaaat ttaaaaccgc aatagaaaaa cacgataaaa cagtgtattg
     6961 cattgcgggt acacgcactt cctttctcaa tgatgctttc ctgaacccgg atcaaccact
     7021 ataccaaatg ggtgatctta tccaattgga tcgcatcgct cttgatgagc tggcaccact
     7081 gatcatgaat cgtttttctg aaagcggaaa atcatgcgct atgatgtttg ccgagcgtat
     7141 ctgcaaggaa atggtttgtc acccctattt cacccagcaa ctggcgcata ttgtttacat
     7201 gaatgccaat ggcaaagtgg acgacgccgt aatttcacgc tcggtagaag aaatgattga
     7261 gcgcaatctc gtatccttcc agcgcgaata cgaaaacctc agtattctcc aggccaactt
     7321 tctgcgaatg ctgctagatg gtgtggaaga tggatttacc accaaaccaa tgatcgaaaa
     7381 atacaaactc agctcctcgg ctgccgtcat tcgtgtgatt gacagtctgc tgaagaaaga
     7441 ggtcattgaa cgccgccagg gtaagttttg ctttgttgat cccacctttc attactggat
     7501 gcagcgcgtt gtaaaaggct gattatctcc ctctcattat tcgattttcc ttagctttgc
     7561 gcctctattc caaaaggcat gtcagcgcaa taccgtacct acgaatcact taacttacct
     7621 tctgttgccc gggagatcc
//
LOCUS       DAOMYG010000104         7627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3213_length_7627_cov_7.053222, whole genome shotgun sequence.
ACCESSION   DAOMYG010000104 DAOMYG010000000
VERSION     DAOMYG010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3213_length_7627_cov_7.053222"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1554
                     /locus_tag="PK610_04715"
     CDS             169..1554
                     /locus_tag="PK610_04715"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRJ35264.1"
                     /translation="MKPITVISLLLIGQITFGQNNVHKIDSIMTSCFSQGNFNGNVLI
                     FEKDQVIYKKSFGFSNESTKEKLNENSIFELASVSKQFTATAIVLLKEKGKLSYEDEI
                     SKYIPELSDYSKITIRNLLTHTSGLPDYMEIMDSTFDKNKIATNKDIISTFSKLKPKT
                     LFEPNTKWEYSNTGYALLASIIEKVSGMTYGDYLNKAIFKPLKMKNTSVYRRRYEPRI
                     IPNYAYGYVYSDSLKKYVLPDELDELKMVIWLDGIVGDGTVNSTVVDLLKWDRALRSN
                     KFISKESKDEIFTSAEINDKTKTNYGFGWVIEDNGVYGNIVSHTGGWPGYMTLIERHL
                     QNDKTIIVLMNYVHNTTNLPVAELRRALYNIEPHKFIALTNEETELYAGNYMNSSGRV
                     IKLICENGVLFRPTDNGEKFELKAISSTKFVIMNYYPEIFYEFILKGNKVEKLLMTQP
                     ELKFSRELIRQ"
     gene            1581..2480
                     /locus_tag="PK610_04720"
     CDS             1581..2480
                     /locus_tag="PK610_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRJ35265.1"
                     /translation="MKSGITLLVCLLHPLLVLSKEKVKLSDLEKFAPTEIYPEDVFLD
                     TTTNKRAMVIVAHDDDDCAMSGTLFRLKQKGWIIMQICLQQHPSKNGKLPARFLYHHY
                     RKILSNTNYRPGKDTTTLSYMPIPYEMIDRQYLTQVIGDTLISIINNFKPSVLFSLDD
                     VKGGYGHPDHIFMSRLVKSLLQSGKIPPVKIYQSVFTPHMEKEIVNTWLKARLDKWGF
                     PHPGLEADKLYEITGMPEPTVQVDMEANGSEKMRYLNAYPKDVRRTLSKFIPYFTEFK
                     AKEYFRLFNREFFRIVDPVKVHR"
     gene            2612..4330
                     /locus_tag="PK610_04725"
     CDS             2612..4330
                     /locus_tag="PK610_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HRJ35266.1"
                     /translation="MTNGKAGGKHPKGLYVLFFTEMWERFGYYLMLGIFSLYMLDSAA
                     NGGMGFDKATKSDIYGTYLGLVYLTPFIGGLLADRILGYRRSVVIGGLLMAAGYFGLG
                     LNNDVGTLYISLLLIILGNGFFKPNISTLVGNLYNNDQYRANKDAGYNIFYMGINIGS
                     FICNFVAAYFRINFGWGWAFWAAGFGMLLGLAIFMIWTKHVKEADVIKPVQKEDKSIG
                     YILASVFIPLFIMGIIGWFIPGNLLGSDANDAFIFGCIPVVLFYVMLWKRASEEDKKP
                     IAALLTVFACVVVFWAVFHQNGDALTVWAEEYTDREIPKPAESVFTTLKIDQTVVNDP
                     DLFAVPDSTFKKRMKSGEERIQALIKANDKTEYKLIQDSLALESHSRTYFQNLPEEKR
                     PPVGQNLKLISTEIFQSINPFWVVLLTPIIVGFFGFLRRRNLEPSTPAKIAYGLLITG
                     ISALVMVMAVWSVDIHDEKASAWWLINSYLVITIGELFLSPMGLSLVSKLSPPRLTAL
                     MMGGWFLSTSVGNKLSGMLGGLWEGIDNKANYFFINFALCALAFVLLFSQLKWLKKTM
                     QDKNIF"
     gene            4363..5847
                     /locus_tag="PK610_04730"
     CDS             4363..5847
                     /locus_tag="PK610_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019974842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HRJ35267.1"
                     /translation="MSKELTLEEIQNFEGKYPKQLWYLFLTEMWERFSFYGMRGMLTV
                     FMVTQLMFPEKDANLKYGAIQAFVYAFTFIGGLFADKILGFKKSLFWGGSLMIIGSMI
                     LAFSPVDLFFIGISFTVIGTGFFKPNISTMVGALYKEGDSRRDAGFGLFYTGINLGAF
                     LGGILCVYIGKFHSWNIAFSLAGIVMTIGLITFLFTQKTMGPIGDSPLKALTKQKRNL
                     REYLVYAVSLACIPFILIMVQNTEYTDLFMYIIGPCTLIYLGYEMTKLSAPEVKKLLA
                     ALVFIIFSIVFWAFFEQSGGSLSLFALYNLNDTLLGVNGVDPNVINNSANSVFVIAFS
                     ALLGLLWLWMAKRKIEPNTLIKFGLGFLFLALAFYIFYATKFFADENGKTSLDVFTFA
                     YLVITFGELCLSPIGLSIMTKLAPKHLWGVMMGMWFLASAYGQYVAGILGAGMTSPDE
                     NATAAVKLEAYTSGYEQLALYALICGGVLILISPFVRKLMQEVK"
     gene            5994..>7627
                     /locus_tag="PK610_04735"
     CDS             5994..>7627
                     /locus_tag="PK610_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase/hydrolase"
                     /protein_id="HRJ35268.1"
                     /translation="MSVKHLILLSSTVLLAMCSPKTEEHKSDASTNEDATRIKSKHLN
                     VKDEHSYANIDKVYATHLFLDINLDFDKKLVKGVATYDITNEGGDTIIFDSKFLAIEK
                     IVIDDKTEATFALGEDDELLGTPIFVPISRDAKKVAIHYSTTDHCEALQWLTPEQTAG
                     KKQPYLLTQGEAILTRTWIPIQDTPGNRITYKAKVSAPKDIMVVMSATNPTEKSADGV
                     YNFTMDQPIPSYLIALAAGDLVFKPIGNRTGVYTEPSMLDKCADELADTEKMLEAAER
                     LYGPYKWERYDIIVLPPSFPFGGMENPRLTFATPTIIAGDRSLTSLIAHEMAHSWSGN
                     LVTNATWNDFWLNEGFTVYFERRIMEELYGKEYAEMLAQLGYQDLLNEVEGLAGSPDD
                     TKLKLDLSDRNPDDAMSEIAYEKGAFFLRMLEETAGRVKFDEFLRNYFTEHSFQPMTT
                     EIFLDYLDKNLVKKNNLKVNVDEWVHGRGIPSNISMINSSKFKAVDTEIGRWTTGTPA
                     KDLATKGWSTHEWMHFLKNIPQVLNKNQLKDLDNSFG"
BASE COUNT         2360 a         1565 c         1535 g         2167 t
ORIGIN      
        1 aattaagtca taaaaaagat ggcatgcaaa agaagcattc caaacaacct gtttcaaacg
       61 ttcaaatcca cttcctgagt atgcaaaggt tttttttagg aacctgatta caaaaacaaa
      121 aaccttcggg cattaacaca cttgtttaaa aatttaattg gaaaaaaaat gaaacctata
      181 accgttatat cacttctgct tattgggcaa atcacctttg gacaaaataa tgttcacaaa
      241 attgacagca taatgacttc atgctttagt caaggaaact tcaatggtaa tgtattgatt
      301 tttgaaaagg atcaagtgat ctataaaaaa tcatttggat tttctaatga aagcacaaaa
      361 gaaaagttaa atgagaattc catatttgaa ttggcttcag tatcgaaaca attcacagca
      421 actgcaattg tattacttaa agaaaaaggc aaactgagtt acgaagatga aatttcaaaa
      481 tatattcctg aattatctga ttactctaaa atcaccatca gaaacctgct tacccataca
      541 agcggcttgc ctgattacat ggaaataatg gatagcacat ttgataaaaa taaaattgct
      601 accaataaag atataatctc aacattttct aaactaaaac ccaaaacatt gttcgaacca
      661 aatactaaat gggaatacag caatacaggg tatgcattat tagcgtccat tatagaaaag
      721 gtatcaggaa tgacgtatgg cgactattta aataaagcca tatttaagcc tctgaaaatg
      781 aaaaacactt ctgtttacag aagaagatat gaaccccgaa ttatccccaa ttatgcatat
      841 ggttatgttt attcggatag tttaaaaaaa tatgttttac cggatgaatt agacgaatta
      901 aaaatggtga tttggctgga tggaattgtt ggggacggaa cagtaaactc aactgttgtt
      961 gatttactta aatgggatag agctctgcgc agtaataaat ttatttcaaa agaatcaaaa
     1021 gatgagattt tcacttcggc ggaaataaat gataaaacaa agaccaatta tggttttggc
     1081 tgggttatag aagacaatgg cgtctatgga aacatagtta gtcataccgg aggctggccg
     1141 ggatacatga cattaattga gagacatctt caaaatgata aaacaataat tgtcctaatg
     1201 aattacgttc acaacactac taacttaccg gtagcggagt taagacgtgc actctacaac
     1261 attgaacctc ataaattcat tgcacttaca aatgaggaaa cagaactata tgccggaaat
     1321 tatatgaaca gctccggaag agtaataaaa ctgatctgcg aaaatggagt gctttttaga
     1381 ccaactgata atggtgaaaa atttgagctg aaagccatat caagcaccaa atttgtaata
     1441 atgaattatt atcctgaaat tttttatgaa ttcattttaa agggcaataa agttgaaaag
     1501 ttactaatga ctcaacctga attgaaattt tcacgcgaat taatcagaca ataaaaactc
     1561 cacctgacat ttaacagatc atgaaatccg gaataaccct attagtctgt ttgcttcatc
     1621 ctttattagt attgagcaag gaaaaggtca aactcagcga cctggagaaa ttcgccccga
     1681 cagaaatcta ccctgaagat gtttttctgg ataccaccac caataagcgg gcaatggtga
     1741 tagtagcgca cgacgatgat gattgtgcga tgtcgggcac tttgttcagg ctaaaacaga
     1801 aagggtggat catcatgcaa atctgccttc agcaacatcc ttcaaaaaat ggaaagcttc
     1861 ctgcacggtt tctttaccat cattacagaa agatcctgag caacaccaat tacagacctg
     1921 gtaaagacac cactactcta tcctacatgc ccatccccta tgaaatgata gaccggcaat
     1981 atttaacaca ggtaattgga gatacgctta tttcgatcat caataatttc aaaccaagtg
     2041 tacttttttc cctcgacgat gtaaagggtg gttacggaca tcctgaccat atatttatga
     2101 gtcgactggt aaaatccctc ctgcagagcg gaaaaattcc tccggtaaaa atctaccaat
     2161 ccgtattcac gccccatatg gaaaaggaaa tcgtaaatac atggttaaaa gcaagactcg
     2221 acaaatgggg atttccccat ccgggtttgg aagcggataa actttatgaa ataacaggaa
     2281 tgccggagcc cacggttcag gtcgatatgg aagcaaacgg cagcgaaaaa atgcgttacc
     2341 tcaacgctta tcctaaagat gtccgccgca cgctgagcaa attcattcct tactttaccg
     2401 aattcaaggc gaaagagtac ttccggcttt tcaatcggga atttttcagg attgttgatc
     2461 cggtaaaggt tcacagataa aaaagacatg aacaaacccg ctgaataagt tgattatcaa
     2521 ccctcaacaa ctgaaaatat cgcattgagg gcttcacgga ttattccctc tttttcctat
     2581 attggcactc ctaaaaaact acggacagcc aatgaccaac ggaaaagcag gtggcaaaca
     2641 ccctaaggga ctttatgttc tcttcttcac cgaaatgtgg gaacgtttcg ggtactacct
     2701 catgctgggg attttctccc tgtacatgct cgattcggca gcgaatggag gaatgggatt
     2761 cgataaagca accaaatctg atatatacgg aacctatttg ggacttgttt acctcacccc
     2821 ttttattgga ggattgcttg ccgacagaat tctgggttat cgcagatccg ttgtgattgg
     2881 aggcttattg atggcagcag ggtatttcgg gttgggatta aacaatgatg ttggaacact
     2941 ctacatttcc ttattgctca ttattcttgg taacggtttt ttcaagccga acatttcaac
     3001 tcttgttgga aatctgtaca acaacgatca gtaccgcgcg aacaaagatg cgggttataa
     3061 tatcttctat atgggaatca acattggttc cttcatttgc aatttcgtag ctgcttattt
     3121 ccgtatcaac ttcggttggg gttgggcatt ctgggccgcc ggattcggga tgctacttgg
     3181 tctcgcgatt ttcatgatct ggaccaaaca cgtaaaagaa gcagacgtca tcaaaccggt
     3241 tcaaaaagag gataaatcca ttggatacat ccttgcatcg gtatttattc cattgttcat
     3301 tatggggatc ataggctggt tcattcccgg caatctgttg ggttctgatg ccaatgatgc
     3361 atttattttc ggatgtattc ctgttgtgtt gttctacgtg atgctttgga aacgggcttc
     3421 tgaagaagac aaaaaaccaa ttgctgcatt gcttactgtt tttgcatgtg ttgtggtttt
     3481 ctgggctgtt tttcaccaga atggagatgc acttactgtt tgggcagaag aatacaccga
     3541 ccgtgaaatt ccaaagcctg cagaaagcgt tttcactacc ctgaaaattg atcaaaccgt
     3601 tgtaaacgat cccgatcttt ttgctgttcc cgactccact ttcaaaaaaa gaatgaaatc
     3661 aggtgaagaa agaattcagg cattgatcaa ggcaaacgat aaaacagaat acaaactgat
     3721 tcaggattca cttgcacttg aaagtcattc gcgaacctat ttccagaatt taccggaaga
     3781 aaaacgtcca ccggtaggac aaaacctgaa actcatttct actgaaattt tccagtcgat
     3841 caaccccttc tgggtggtgc tacttacccc gatcattgtt ggatttttcg gattccttcg
     3901 tcgcagaaac ctggagccct ccacacctgc aaaaattgcg tacggactcc tgatcacagg
     3961 aatctctgca cttgtgatgg taatggccgt atggagtgtc gatattcacg atgaaaaagc
     4021 atcggcgtgg tggcttatca actcgtatct ggtaattaca ataggtgaat tattcctctc
     4081 accaatggga ttatcactgg tatccaaact ctccccgccg cgattaactg cattgatgat
     4141 gggaggatgg ttcctttcaa catctgtcgg aaacaaacta tccggaatgc tcggcggact
     4201 atgggaagga attgacaata aagccaatta cttctttatc aatttcgcat tgtgcgcact
     4261 tgcatttgta ctgttgttct cacagctgaa atggctcaag aaaaccatgc aggacaaaaa
     4321 catcttttaa ccgatcatcc ttaaatctga attcgcaccg ctatgagtaa agaattgaca
     4381 ctggaagaaa tccagaattt tgaaggaaaa tatcccaagc agctttggta tttgttcctc
     4441 accgaaatgt gggagcgctt ttccttttat ggtatgcgtg gaatgctaac cgtttttatg
     4501 gtaacacaac ttatgtttcc tgagaaggat gccaacttga aatatggtgc aatacaagca
     4561 ttcgtatatg cattcacttt tattggcgga ctatttgccg acaaaatact tggtttcaag
     4621 aaatcccttt tctggggtgg aagccttatg attatcggaa gtatgattct tgcattttcc
     4681 ccggtggatc tgttttttat tggcatcagc tttaccgtta tcggaacagg tttcttcaaa
     4741 ccgaatattt caacaatggt tggagcactt tataaagagg gtgattccag aagagatgca
     4801 ggttttggtt tattctacac cggtattaat ctcggtgcat ttttgggagg aatcctctgt
     4861 gtgtacattg gtaaattcca ttcctggaac attgcattct ctcttgccgg aatagtaatg
     4921 acaattggtt tgatcacctt tttgttcact caaaaaacaa tgggtcctat tggtgattct
     4981 ccactaaaag cccttacaaa gcagaagaga aatttgcgcg aatatcttgt ttatgctgtt
     5041 tctctcgctt gcatcccctt tattctgata atggtccaga acacagaata tactgatctg
     5101 ttcatgtata ttattgggcc ttgtactttg atttatttag gttatgaaat gacaaaacta
     5161 agtgcacctg aggttaagaa gcttttggca gcgctggtct ttattatttt ctccatcgta
     5221 ttctgggcat tctttgagca gagtggcgga tctcttagct tgtttgctct atacaatctg
     5281 aacgacactt tacttggagt taatggtgtt gatcctaatg taatcaacaa ctctgcaaac
     5341 tcggtatttg taattgcatt cagtgcatta ttaggactcc tttggttatg gatggcaaag
     5401 cggaaaatcg aacccaatac acttattaaa ttcggactcg gcttcttatt ccttgcactt
     5461 gcgttctaca tattctacgc aacaaaattc tttgcagatg aaaatggaaa aacatcgctt
     5521 gacgtattta cgtttgccta cctggtcatt acgtttggtg aactttgttt atctcccatc
     5581 ggactatcca ttatgaccaa actcgcacca aaacatttgt ggggcgtaat gatgggtatg
     5641 tggttccttg ccagtgcata cggtcagtat gtagcaggaa tacttggtgc aggaatgacc
     5701 tcaccggatg agaacgccac agccgcggta aaactggaag cctacaccag cggttacgag
     5761 caattagcct tgtatgccct catttgtggt ggcgttttaa tacttatttc gccatttgtg
     5821 cgtaaactca tgcaggaagt caaataagct tttaccctca ttttcaaagc ctcccgcgtt
     5881 aagtgggagg cttttttatt tattcatcgt ccgaaatgag aaggttatcg aaatgggact
     5941 tgtttcattt gcgtaaactt ctaatttagc gcaccctaaa caaacacagt ccaatgtcag
     6001 taaaacacct aattcttctg agcagcaccg ttcttttggc gatgtgctca ccaaaaacag
     6061 aagagcacaa atccgatgca tccaccaacg aagatgcaac gcgcatcaag agcaagcacc
     6121 ttaacgtgaa ggatgagcac agttacgcaa acatcgataa agtttacgca acccatttgt
     6181 ttctcgacat caaccttgat ttcgataaga agctggtaaa aggagttgct acatacgaca
     6241 tcaccaacga aggtggcgac actattattt tcgacagcaa gtttttagcg attgaaaaaa
     6301 ttgtgatcga cgataaaacc gaagcaactt ttgcactggg agaagatgac gaacttttgg
     6361 gaacacccat ttttgttccg atcagccgcg atgcaaaaaa ggttgcgatt cattacagca
     6421 caacagatca ttgtgaggca ttgcaatggc tcacacctga acaaactgcc ggtaaaaaac
     6481 aaccatacct gctcacacag ggagaggcta ttcttacccg tacatggatt ccaattcagg
     6541 atacaccggg taaccgaatt acctacaagg caaaagtatc tgcacccaaa gacataatgg
     6601 ttgtgatgag tgcaacaaac cccaccgaaa aatcggctga tggggtgtac aatttcacta
     6661 tggaccaacc gattccttct tacctgatcg cacttgcagc aggtgattta gtattcaaac
     6721 ccattggaaa ccgcaccggt gtttatacag aaccttcgat gctggataaa tgcgcagacg
     6781 aacttgccga tacagaaaaa atgctggaag cagctgagcg tttgtacggt ccttacaaat
     6841 gggaacgtta cgacatcatc gttcttccac caagtttccc tttcggcgga atggagaatc
     6901 cacgtttaac atttgcaaca ccaaccatta tcgcgggcga ccgttcgctc acttcgctaa
     6961 tcgcacacga aatggcgcac agctggtcgg gtaatctcgt taccaatgca acatggaacg
     7021 atttctggct gaatgaagga tttacagtgt acttcgaacg tcgtatcatg gaagaacttt
     7081 acggcaagga atatgctgaa atgcttgctc aactgggtta ccaggatctt ctcaatgaag
     7141 tggaaggcct tgcaggatca ccggatgata ctaaactgaa actcgacctt tctgaccgta
     7201 atccagatga tgcaatgtcg gaaattgctt acgaaaaagg agcattcttt ttacgtatgc
     7261 tcgaagaaac tgccggtcgc gttaagttcg atgaattcct gagaaattat ttcactgagc
     7321 attcctttca accaatgacc actgagattt tccttgatta cctcgataag aaccttgtga
     7381 agaaaaacaa cctgaaagtg aatgttgacg aatgggttca tggtcgcgga attccttcca
     7441 atatctcgat gatcaactct tctaaattca aagcggttga tactgaaatt ggacgctgga
     7501 ctaccggaac ccctgcaaaa gacctcgcta ccaaaggttg gtcaacgcat gagtggatgc
     7561 acttcctcaa aaacattcca caggttttga ataaaaatca gttgaaagac ctggataaca
     7621 gctttgg
//
LOCUS       DAOMYG010000105         7575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3247_length_7575_cov_10.503191, whole genome shotgun sequence.
ACCESSION   DAOMYG010000105 DAOMYG010000000
VERSION     DAOMYG010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7575
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3247_length_7575_cov_10.503191"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /locus_tag="PK610_04740"
     CDS             <1..1614
                     /locus_tag="PK610_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HRJ35269.1"
                     /translation="KVSGGLHGVGVSCVNALSTHLKAEVHRGGKQYVQEFSIGKPLYS
                     VKEVGTTDYRGTIVTFKPDESIFFVHEYKYDTLATRLRELAFLNKGVNLRLTDRRETN
                     ENGEFRNDEFYSQGGLKEFVRFLDQNREALMSDVMYMEGEKAGIPVEVAMVYNTSYSE
                     NIHSYVNNINTHEGGTHLAGFRRGLTRTLKKYADDSGMLKNLKFEISGDDFREGLTAV
                     VSVKVQEPLFEGQTKTKLGNNEVMGAVDQAVGEMLNNYLEENPKDARQIVNKVILAAT
                     ARHAARKAREMVQRKSVMGGTSLPGKLADCSERDPAACEVFFVEGDSAGGTAKQGRDR
                     RFQAIMPLRGKILNVEKAMYHKIFENEEIKNIYTALGVRVGTEEDSKALNLSGLRYHK
                     VVIMTDADVDGSHITTLILTFFFRYMKELIENGYIYIATPPLYMVKKGQTFRYAWNDE
                     QRDQYINELKGQGKDSSVNVQRYKGLGEMNAEQLWETTMNPEARTLRQVTIDNAAECD
                     RVFSMLMGDDVPPRREFIEQNAKYAKIDI"
     gene            1770..3710
                     /locus_tag="PK610_04745"
     CDS             1770..3710
                     /locus_tag="PK610_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HRJ35270.1"
                     /translation="MKHKWLLLLLLPVHVMAGELELKTSIKDVTVFQTGAQVTRNGIT
                     SVPAGESEIHLKDIPPGLKRESIQVRSNAEITLLSVSFETITNDVEPDREKNKLLEKK
                     KEEYEIRLEDMLARFSILQAEEQIISNLQQVSTTSTGVNVDQIVKAQEIMRTKMNLII
                     TEQLTLKRKIKDQQESLREVSQQISVLNGTKQTLSNDIVIRVSAKQETRADFSVSYIM
                     PNARWIPAYDIRVKNVGEPMQIEYKAQVQQHTGEDWSKVKLKLSTGDPSLGSKKPSIV
                     PWYINLNQPYQQPQQQSNFYKYTDAKFTKVSGRVFDAENGEALFAASVIATGTNIGVS
                     TDFDGKFSLTLPENASSLTVKYIGYNDQVIALTASEFNIYLKPSVVELAEVVVMQESA
                     RGRDNHSAQSISYNSVGSKSSGATVSREDIRTTAVRGSRSAASMPQVQPQLNIVSTEF
                     VITESYSIPSGQKPVSVTIQTVQTPAHYQYYCAPRLDKDVFLTAQIVDWEQYNFLEGN
                     ANVFFEGTFVGNTLMETKYLSDTLNISLGRDKSVKVERKKSKEYTKRQIMGGDQFTYR
                     HWDFTVRNGKSQKINIILEDQFPLAANSSIEIRREEKSGGDLNEETGIVTWKFDLDPQ
                     KNKELKLRYSVKHPKGTFVGLD"
     gene            complement(4030..5979)
                     /gene="yidC"
                     /locus_tag="PK610_04750"
     CDS             complement(4030..5979)
                     /gene="yidC"
                     /locus_tag="PK610_04750"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRJ35271.1"
                     /translation="MDRNSVIGLVLIAVILVVFSVMNAPSEEERLRAAQAQDSIARAE
                     AAIAKKEIITVEQDTVATTATLSAANDSTTVVAATGDSTTLALRDSILKAEENLRLAN
                     RYSIFAAAAQGEKTYHVLENDRLRLTFSNKGGQIVSAELKNYETYYNFTDKDSNIPLK
                     LFDEDSSSMGISFQVNGIQISTLDLFASTSAPEVQTTENADSVSLVYRFGTNDPEKFI
                     ELTYSLNRGTFVMGMQVQVNGLGKEDVPNTLELNWSSKLLSTERLVENERMLSSVFYK
                     YTDAGRTYLSETSSDKADLEKPTSWIAFKQSYFSVVLMSDKGISDDGSSVAVNQLTSA
                     KYIKEYVADLNIGSENSEKVNVPLKFYFGPNDIKELSQFNNGMEDIVNLGWGIFRWTN
                     KYLVIPIFNVLDSMNWNYGIIILILTLIVKIIILPLTYKNYKSSAKMRVLKPEIDEIT
                     KKMGSDDPMAKQQATMALYRSSGVNPMAGCFPMLLQMPVLIAVFRFFPSAVQLRQTGF
                     LWADDLSGYDSILDFGFNIPLYGDHISLFTLLMAVSTILITRMNSGQMDTGMPGMKFM
                     MYFFPIMMIFFFNNMSSGLSYYYFVSNVLSLAIMWGIKKWFIDEKKILAQIQANRKNP
                     KAQKKSAFMQRLEEAQRMQQQKKKK"
     gene            6252..7022
                     /locus_tag="PK610_04755"
     CDS             6252..7022
                     /locus_tag="PK610_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRJ35272.1"
                     /translation="MAMFLDLVWRHRFGVLGTLFLHLAFVIYSRNQVVFVYEPTVDFI
                     DMSAVPIQLLPPLEPQKQPEQQLDANGNVINKEFNAADQRENSEQNYDARFNKSNVEK
                     SVYDELKAFEKAEFDKLQSGKGNPGENNSKEVDITQQHKTDNNKQEKDPNSNQGTSSG
                     ALKGTTTVEYDLGGRRHEKMSKPSYTCIGSGTIVMKIKVNRSGKVTGASIDNSQSQIP
                     EECMREAALTYVYRAKFEASATAPEPQEGTITYRYIQQ"
     gene            complement(7267..>7575)
                     /locus_tag="PK610_04760"
     CDS             complement(7267..>7575)
                     /locus_tag="PK610_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRJ35273.1"
                     /translation="SPLWKLPKIETWMDHNAHHFFILCKDQSTRDRIRKHLAEKNIAS
                     SFHYIPLHTSSFISKQNAQHVSLPNAERFGETLLRLPLFNTLTEQEVDEVCKAVLGEQ
                     "
BASE COUNT         2310 a         1507 c         1767 g         1991 t
ORIGIN      
        1 aaggtttccg gtggattgca cggtgtgggt gtttcctgtg tgaatgcact ttctactcac
       61 cttaaagctg aggttcaccg tggaggaaaa caatatgtgc aggaattcag cattggtaag
      121 ccgctgtatt ccgttaagga agtaggaaca acagattacc gtggtaccat tgtaacgttt
      181 aagcccgacg aatcgatttt cttcgtgcat gagtacaagt acgatacgct tgcaacgcgc
      241 cttcgtgagc ttgcgttcct aaacaaagga gtgaatcttc gtcttaccga ccgtcgcgag
      301 accaacgaga atggtgagtt ccgtaatgat gagttttata gtcagggagg cctaaaggag
      361 tttgttcgat tcctcgatca gaaccgtgaa gccctgatga gtgatgtgat gtacatggaa
      421 ggcgaaaagg caggaattcc tgttgaagtg gctatggtgt acaacacatc ctatagcgaa
      481 aacattcact cctacgtaaa caatatcaat acccacgaag gcggaacgca ccttgccggt
      541 tttcgtcgcg gattgacacg tacgcttaaa aagtatgccg acgactcggg aatgcttaag
      601 aatttaaagt ttgaaatttc gggtgatgac ttccgtgagg gattaaccgc tgtagtttcg
      661 gtaaaagtgc aggaaccatt gtttgaaggt cagaccaaaa ccaaactcgg aaacaacgag
      721 gtgatgggtg cggtagacca ggcagtaggt gagatgctga acaactatct ggaggaaaat
      781 ccaaaagatg cgcgtcagat tgtaaataaa gtaatcctcg ctgcaactgc acgtcatgct
      841 gcccgtaagg cgcgtgagat ggtacagcgt aagagtgtta tgggaggaac atcacttccg
      901 ggtaagctgg ccgactgttc tgagcgtgat cctgcagcct gcgaagtttt ctttgttgag
      961 ggtgattctg caggtggaac agccaagcaa ggacgtgacc gccgattcca ggccatcatg
     1021 ccattgcgcg gaaagatcct taacgttgaa aaagcgatgt accacaagat cttcgaaaac
     1081 gaagagatca aaaatatcta tacagcttta ggtgtgcgtg ttgggacgga agaagacagc
     1141 aaggcattaa atctgagcgg acttcgttac cacaaagtag tgatcatgac cgatgcggac
     1201 gtggacggtt cgcacattac tacgctaata cttacgtttt tcttccgcta catgaaagag
     1261 ctgattgaaa acggatacat ctacattgcc actccgccgc tttacatggt gaaaaaaggt
     1321 cagactttcc gttacgcatg gaacgacgag caacgcgacc aatacatcaa tgagttaaaa
     1381 ggtcagggta aagacagcag tgtaaacgta caacgctata agggtcttgg tgaaatgaac
     1441 gcagaacagc tttgggaaac aaccatgaat ccggaagccc gcacattacg tcaggttact
     1501 atcgataatg ctgcggaatg tgatcgtgta ttctctatgc tcatgggtga tgatgttccc
     1561 ccacgcaggg aattcattga acagaatgcc aaatacgcga agattgacat ttaaaacagt
     1621 tccatacaaa tgagtaaaag agcaggccac gtgtctgctc ttttttttgg taacaatttc
     1681 ttcatacacg aaacagacaa ttagccatcg ttttttggtt tagggttatg gaaacagatt
     1741 caaagctgca tccactaaaa aaaacgatca tgaaacacaa atggcttttg ttgctgctgt
     1801 tgcctgtgca tgttatggca ggtgaactgg aattaaaaac aagcattaaa gatgtaacgg
     1861 tattccagac cggtgcgcag gttacacgaa acggaatcac ctccgtaccc gcaggtgaat
     1921 cggaaattca tttaaaggat attcctcccg gactaaaacg tgaaagtatt caggtaagat
     1981 cgaatgctga aattaccttg ctatccgttt catttgagac catcacaaat gatgttgaac
     2041 ctgacaggga aaagaacaaa ctgttggaga agaaaaagga agaatacgaa atccgtcttg
     2101 aagatatgct cgcacgattc agtattcttc aggcagaaga acaaatcatt tccaatcttc
     2161 agcaggtgtc taccacttct acgggagtga atgttgatca gatagtgaaa gcgcaggaaa
     2221 tcatgcggac aaaaatgaat ctgatcatta ctgaacaatt gactttaaaa cgaaaaatta
     2281 aagatcaaca ggaaagcctg cgagaagtca gtcagcaaat ttcagttctc aacggaacaa
     2341 agcaaacatt gagtaatgat attgtaatac gcgtttcggc aaaacaggaa acgcgtgctg
     2401 atttttcagt ttcgtacatc atgcccaatg cacgatggat ccctgcttat gatattcggg
     2461 taaagaatgt tggtgaaccc atgcagatcg aatacaaagc gcaggtgcaa caacacaccg
     2521 gtgaagattg gagtaaagtc aaactcaaac tttccacggg agatccatca ctaggatcca
     2581 aaaaaccaag cattgttccc tggtatataa acctaaatca accctaccag caaccgcaac
     2641 aacaatcaaa cttttacaaa tacaccgatg caaaattcac caaggtgagt ggaagagtgt
     2701 ttgacgcaga aaacggagaa gcattgttcg ctgcgtcggt aattgcaaca ggaacaaata
     2761 ttggcgtctc taccgatttc gacggcaaat tttcacttac gctaccggag aatgcaagca
     2821 gcttaactgt aaaatacatt gggtataatg atcaggtgat tgcacttaca gcatcagagt
     2881 ttaatatcta tctcaaacct tccgttgttg aattggctga ggttgtggtt atgcaggaat
     2941 cagcaagagg cagagacaat cattctgcac aatcgatcag ctataattcg gttggttcaa
     3001 aatcttccgg cgcaacagtt tcccgcgagg atatacggac aacagcagtt cgcggatcgc
     3061 gttctgcagc ctccatgcct caggtgcaac cgcagttaaa tattgtgagc acagaatttg
     3121 ttatcaccga atcctattcc attccttccg gacaaaaacc ggtaagcgtt accatccaaa
     3181 cagttcaaac tccggcgcat tatcaatatt actgtgcacc ccgacttgat aaggatgttt
     3241 ttctaacggc gcaaattgtc gattgggagc aatataattt tctcgaagga aatgcaaacg
     3301 tgtttttcga aggaacgttt gttgggaata cattaatgga aacgaaatac ctgagtgata
     3361 cacttaatat ttcgctggga agagacaaat ccgttaaagt agagcgtaaa aagtcgaagg
     3421 agtataccaa acgacaaata atgggtggcg atcagttcac ctatcgtcat tgggacttta
     3481 cagtgcgaaa cggaaaatcg cagaaaataa atatcattct tgaggatcag tttccgcttg
     3541 cagcaaactc ttcgatagaa attcgtcgcg aagaaaaaag cggtggagat ctcaatgaag
     3601 aaaccggaat tgtaacctgg aagtttgatc tcgatccgca aaagaacaaa gagctgaagc
     3661 ttcgctattc agtaaagcat cctaaaggaa catttgtagg attggattga ggatttaatg
     3721 tccgagatga tgattctcgt tgtgaagaga ggaatacttt ggagttacta ttggggaatt
     3781 tagagtgtaa cccgggggcg aatggagttt tctatgagga tatattgagt atagccggga
     3841 tgttcttgtt agcttaatcg ggtgctgtaa tggggtttct ctatcaggat gatgtggtta
     3901 tatctaagat cttttttgaa ttagccggtt gctgaagcac ccggttacgg atggaaggtg
     3961 gcttaatgac acagggactg aagcccctgt gagaaagaaa tgattgtttg aggcattggc
     4021 ctcaaataat catttctttt tcttctgctg ttgcatacgc tgcgcttctt cgagtcgctg
     4081 cataaaggca gatttctttt gggctttcgg attttttctg ttggcctgta tctgtgcaag
     4141 aatttttttc tcgtcgataa accatttttt aatcccccac ataattgcaa gtgataaaac
     4201 gttggagacg aaataataat aactcagacc agatgacatg ttgttaaaga aaaagatcat
     4261 catgataggg aagaagtaca tcatgaattt catgcccggc atgccggtgt ccatctgacc
     4321 gctattcatt cgggtaataa gaatagttga aacagccatc aacaaagtga acagagaaat
     4381 gtgatcgccg tagaggggaa tattaaatcc gaaatcgaga atagaatcgt aaccggaaag
     4441 gtcatcagcc caaaggaatc cggtctgacg aagttgaact gcagatggaa aaaagcggaa
     4501 tacagcaatc agcacgggca tctgtaagag catcgggaaa caacctgcca ttggatttac
     4561 acctgagctg cggtaaagtg ccatcgtcgc ctgttgcttg gccattggat cgtcgcttcc
     4621 catttttttg gtgatttcat caatctccgg cttaagcaca cgcatttttg cactcgactt
     4681 gtaattttta taagtgagag gaagaatgat gatttttacg atcaaagtaa gaatgagaat
     4741 gatgattccg taattccagt tcatcgaatc aagcacgttg aaaattggaa tgacaaggta
     4801 tttgtttgtc cagcggaaaa tcccccaacc aagatttaca atgtcttcca ttccgttgtt
     4861 gaactgcgaa agttccttga tgtcgttcgg accaaaatag aatttgagcg gaacatttac
     4921 tttttccgaa ttttctgaac caatgttgag gtcagcaaca tattccttga tgtattttgc
     4981 agacgtaagt tgattcacgg caacagaaga tccatcatcc gaaattcctt tatccgacat
     5041 cagcacaacc gagaaataac tctgtttgaa tgcaatccag gaagtaggtt tttccaaatc
     5101 ggctttatcg cttgatgttt cactcaggta tgttcttccg gcgtcggtgt atttgtaaaa
     5161 aacagaggag agcattcgtt cattctcaac aagcctttcg gtggagagaa gtttgctgct
     5221 ccaattcaat tcgagtgtat tgggaacatc ttctttaccg agtccattta cctgaacctg
     5281 cattcccatc acaaaagtcc ctctgttgag tgaataggtc agttcaatga atttttccgg
     5341 atcatttgtt ccgaagcggt aaacaagcga aacactatcg gcgttttcgg tggtttgtac
     5401 ttcaggagcg gatgtagagg caaaaagatc aagtgtgcta atctgtatgc cattcacctg
     5461 aaagctgatt cccattgacg aagaatcctc atcaaaaagt ttaagtggaa tgttgctatc
     5521 cttatctgtg aagttgtaat acgtctcgta gtttttcagt tcggcagaaa cgatttgtcc
     5581 gcccttattg ctaaacgtta aacgaaggcg atcgttttcc agaacatgat acgttttttc
     5641 accctgcgca gcagctgcga aaatagagta gcgattcgcc agacgcagat tctcttccgc
     5701 tttcaggata gaatcacgaa gagcgagtgt agtgctgtcg cctgtagctg caactactgt
     5761 tgtgctgtcg tttgcagcag aaagggtagc ggtggtagcg acagtatcct gttctacagt
     5821 aatgatttct ttttttgcaa tagccgcttc agcgcgggca atgctgtctt gtgcctgggc
     5881 agcgcggaga cgttcttctt cgctgggtgc attcattact gagaacacta ccagaattac
     5941 tgcgataaga accaatccga tgacggaatt tctatccatt gaggagatga ttgaataatg
     6001 agggggcaaa ggtaatattc aaagagatac tacttcaatc ccgcaaggag attattcgtg
     6061 aaaaggtaag actcaagagt ttaatggctt ggaaaaggct ttttcgggag ttctataaat
     6121 tgaaatgaat acttaatctg ctctttttta accgtttaat cacaattcaa tctgaataga
     6181 atgttctgat gaactgaacg tatcagaaac aaaggggtac ggactacaga tatccttagt
     6241 atttttgaag catggccatg ttcttggatt tggtatggag gcatcgattt ggagtgttgg
     6301 gaacactatt cctgcatctt gcatttgtga tttacagtcg caatcaggta gtatttgtat
     6361 atgagccaac tgtcgatttt atcgatatga gtgcggtacc cattcagtta ttgcctccat
     6421 tggaaccaca aaaacaaccc gaacagcaac tggatgcgaa tgggaatgtc atcaataagg
     6481 agtttaatgc agcggatcaa cgcgaaaatt ctgaacagaa ttacgatgcg cggtttaata
     6541 aatcgaacgt cgaaaaaagt gtttacgacg agttgaaagc attcgaaaaa gcagagttcg
     6601 acaaattaca atccgggaaa ggaaatccgg gagaaaacaa ttccaaggaa gtcgatatta
     6661 cgcagcagca taaaaccgac aacaataaac aggaaaaaga tccgaacagc aatcagggca
     6721 cgagcagcgg agcgttgaaa ggaacaacaa cagtggaata cgatctgggc ggacgtcgtc
     6781 acgaaaaaat gtcaaagcca tcttatacct gtattggaag cggaactatt gtaatgaaga
     6841 ttaaagtcaa ccgcagtgga aaagtcaccg gtgcttccat tgataattct caatcgcaaa
     6901 ttccggaaga gtgcatgcgt gaagcagcat tgacctatgt ttaccgcgcc aaattcgaag
     6961 catctgcaac tgcacctgaa ccgcaggaag gaacgattac ctatcggtac attcagcagt
     7021 gagagggaag cgaaccgcaa atcctccttc gttccacttc ggagaacgta aggagaatgg
     7081 gaagaaggat gttctgaagg gaaacaatgc ttcacttagc gaagcattga aggtgccttc
     7141 ggcactacat aaaggtgtac cactgtaaat tcttaacctg aagaaaaatt ttgaaacggg
     7201 accaagtcct gaagcgaaat cgactgcaag gagatttctg cttcagagcc gttcactgtt
     7261 caccatttac tgttcaccca aaacagcttt acacacctca tccacttcct gctccgttag
     7321 agtattgaac aatggtagtc gaagcaatgt ttcgccaaat cgttccgcgt tgggtaatga
     7381 aacatgttga gcattctgct tggatatgaa gctgcttgtg tgtaatggaa tataatgaaa
     7441 agacgatgcg atattttttt ctgcaagatg tttgcgaatc cgatcgcggg tggattggtc
     7501 tttgcacaga ataaaaaagt gatgtgcgtt gtgatccatc caggtttcaa ttttcggtag
     7561 cttccataac ggaga
//
LOCUS       DAOMYG010000106         7505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3296_length_7505_cov_9.285235, whole genome shotgun sequence.
ACCESSION   DAOMYG010000106 DAOMYG010000000
VERSION     DAOMYG010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7505
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3296_length_7505_cov_9.285235"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PK610_04765"
     CDS             complement(<1..662)
                     /locus_tag="PK610_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35274.1"
                     /translation="MRPNEDLFQLIQSMSREERGYFSKYADIHAKGGENNYKRLFSLI
                     CSIDDPTDEKVRKRLAGDPLLDYFSTAKNQLYFILLRTMRFSQRDLGFVNRTSELLSE
                     CDLLNARGLRNQMQKSIRRAEEFVFSNELVLPALELSDRNFDFHFEDHLGPTDLEQRM
                     ETLFRLREKLMDDLHEQHRMERWLVKMQLAIMRKNLSESTRNAGIRNILALLEQEESR
                     KPS"
     gene            complement(885..2828)
                     /gene="dxs"
                     /locus_tag="PK610_04770"
     CDS             complement(885..2828)
                     /gene="dxs"
                     /locus_tag="PK610_04770"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRJ35275.1"
                     /translation="MPDFKPGPLLSQIDSPLDLRSKFQEEDLPQISQELRDYIIDVVS
                     HIGNSHFGASLGVVELSVALHYAFNTPNDQLVWDVGHQAYGHKILTGRRDAFPTNRTY
                     KGLSGFPKRSESEYDTFGVGHSSTSISAAVGMAVANKYLGKEDLQHIAVIGDGAMTAG
                     LAFEGLNHGGVTNSNLLVVLNDNCMSIDPNVGALKEYLTDITTSHTYNKVKDEVWNLL
                     GKVSKFGPNAQAIAQKISDSIKGSLLKQSNLFESLNFRYFGPVDGHDVINLTRIMKDL
                     KDIPGPKILHCVTVKGKGYKHSEEGNATVWHAPGVFNKDTGEIIKIAPKSPQPPKYQD
                     VFGHSIVELAERNDKIVGVTPAMPSGCSLNIMMKAMPDRAFDVGIAEQHAVTFSAGLA
                     TQGLIPYCNIYSSFMQRAYDQVIHDVALQKLHVIFCLDRGGLVGADGATHHGAYDLAY
                     MRCIPNMIVASPMNEEELRDLMYTAQLEECKGSWSIRYPRGEGVMVNWKTPFKKISIG
                     AGRKVRNGEDIAILTIGPIGNHALKAAEQLAEQGISAAVYDMRFAKPIDEILLHEVFT
                     KFKKVITVEDGCIQGGMGSAVIEFMADNGYSAQVRRLGVPDRFVDHGTQAELYAECEY
                     DQTAMVKHAQEMVQKHKSISIAG"
     gene            complement(2940..3653)
                     /locus_tag="PK610_04775"
     CDS             complement(2940..3653)
                     /locus_tag="PK610_04775"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF001186.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRJ35276.1"
                     /translation="MAYLSALSVLVVKNLPSPTTIIIVAGGSGKRMASAQPKQFLPIH
                     GKAILHHTIEAFHKVDPTFRYVVVLPEEYIDYWKNHAAMKKIPVEQTLVAGGAERFFS
                     VKNALKEVPKSGLVAIHDGVRPLVSKHTIRYALETAAEKGNAIPVLPVVDSVREVFGE
                     STRPVDRSHYRLVQTPQCFDAELIHRAYAQDYYPSFTDDASVVEKLGVHINLVEGNRE
                     NIKITTSFDLKLAEVLLNI"
     gene            complement(3816..4865)
                     /gene="queA"
                     /locus_tag="PK610_04780"
     CDS             complement(3816..4865)
                     /gene="queA"
                     /locus_tag="PK610_04780"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRJ35277.1"
                     /translation="MKLSQFKFKLPPELIANHPAANRDESRLMVLNRETQTIEHRKFR
                     DIMEYFGDGDVMILNNTKVFPARMYGNKEKTGAKIEVFLLRELNRESLLWDVLVDPAR
                     KIRIGNKLYFGDDDSLVAEVIDNTTSRGRTLRFLFDGPYEEFKKTIERLGETPIPKYI
                     KREVEKEDADRYQTVFAQHEGAVAAPTAGLHFSKELMKRLELKGVNFAELTLHVGLGT
                     FRTVEVEDLTKHKMESEQVIIPALCADTVNMAKADRKRICAVGTTVMRGIETSVSTDG
                     FLKPFDGWTNKFIFPPYDFSIANSMITNFHMPMSTLLMMVTAFGGYDFVMKAYEEAIK
                     EEYRFYSYGDAMLII"
     gene            5033..7126
                     /gene="metG"
                     /locus_tag="PK610_04785"
     CDS             5033..7126
                     /gene="metG"
                     /locus_tag="PK610_04785"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRJ35278.1"
                     /translation="MNTAKRHTITAALPYANGPLHIGHIAGAYLPADIYVRYLKMRGE
                     DVAFICGSDEHGAAITIRAKKEGISPREIIDKYHSINKKAFADLGIAFDIFHRTSDPL
                     HHQTAQDFFIQLEKNGAFTKETSEQFFDEEHQQFLADRYITGTCPKCGNENAYGDQCE
                     KCGSSLNPTDLINPKSTLSGKAPVLRKTEHWYLPMERHEAWIREWIEKGTLDGKPHHD
                     PKEWKTHVVGQCKSWIDAGLQPRAMTRDLDWGVKVPVEGAAGKVLYVWLDAPIGYISA
                     TKQWAKDKGKNWEDYWKSPDTRLLHFIGKDNIVFHCIIFPILLKAHGEFLLPENVPAN
                     EFLNLEGDKLSTSRNWAVWVHEYLEELPGKEDELRYVLASIAPETKDSEFTWKDYQAR
                     VNNELVAIFGNFVNRVVVLMEKYYQGIVPEINGSIKTEQEKKLFGELIPSHWKSLEDN
                     MNRYRFRESLAAAMDIAREGNKYLAETEPWKLIKSDTERTKEILFNALQICAQLSVAF
                     EPFLPNTSNKLRGLLNIAPIDWKNAINGNLVSSGHTLGKPGLLFEKIDDIVIEKQMLK
                     LEESRKVYKELTAPSSSTVKTQKSDITFDEFSKMDIRVGTILAAEKVEKADKLLKLTV
                     DTGIDQRMIVSGIAEHYTPEEVIGKKVSVLINLAPRKIRGVESKGMILMAENAEGKLC
                     FISPVNTFDSGSEIR"
     gene            7164..>7505
                     /locus_tag="PK610_04790"
     CDS             7164..>7505
                     /locus_tag="PK610_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35279.1"
                     /translation="MSQAAESTLPYYPFEVRVYDAEIIIAELVQEIEAVEIEHMHALT
                     RALEEMGEGKKWKVVTVIHNYSLLDTKAREYASSEYAQRFTIANAIVVTSLATRIGAN
                     FFINFSKPPRPT"
BASE COUNT         2045 a         1709 c         1763 g         1988 t
ORIGIN      
        1 gaaggttttc gcgattcttc ctgctccaat aatgccagaa tgtttcgtat tccggcattg
       61 cgagtgctct cgctaaggtt tttacgcatg atggccaatt gcatttttac aagccagcgt
      121 tccattcggt gttgctcatg taaatcatcc atcagctttt ctctcaggcg gaaaagggtt
      181 tccatacgct gttcaagatc tgttggaccg agatggtctt cgaaatgaaa atcgaaattg
      241 cgatcactaa gttcaagtgc aggtaaaacc agttcattgg aaaaaacaaa ttcctccgcc
      301 ctgcggatcg atttttgcat ttggtttcgc aatccgcgtg cgttaagtaa atcacattcc
      361 gataataatt cagaagtgcg gtttacaaat ccaagatcgc gctgcgaaaa acgcatggtt
      421 cgcagtaaaa taaaatacaa ctgatttttc gcagtcgaaa aataatcgag tagaggatca
      481 cccgcaagtc gtttcctaac cttttcgtcg gtaggatcat caatactaca aatcaaacta
      541 aataaacgtt tgtaattgtt ttcacctcct ttagcatgta tgtcggcata cttggaaaag
      601 taacctcttt cctcccgcga catcgactgg attaattgaa acaaatcttc atttggacgc
      661 atatcgaatc ttttactggt tgggtggcaa accaataaaa agagattgat aaaaaatgtc
      721 gaattgtaag aatgaaagaa ttttgcgcaa aaagtaaatc gtgatttacg tggcgtttcc
      781 ttacaaaatt tacctcaggt aacaaaaagt aaataaataa ttagcgatta tactaattgc
      841 attagcacta aaaatttcag aaaagaaatc aattggcact tctattagcc agcgatgcta
      901 atggatttat gcttctgcac catttcctga gcatgtttta ccatggcagt ttggtcgtat
      961 tcgcactcgg cgtacaactc ggcctgtgtg ccatggtcta caaaacgatc gggcacaccc
     1021 aaacggcgaa cctgagcgct gtagccgttg tcggccatga actcgatcac tgcacttccc
     1081 attccgccct ggatgcaacc gtcttccacg gtgatcactt ttttgaattt ggtgaagact
     1141 tcatgcaaga ggatttcatc gatgggcttg gcaaaacgca tatcgtatac cgcagcagaa
     1201 atgccttgtt cggcgagttg ttctgcggct ttgagggcat gatttccgat cggaccaatg
     1261 gtgagaatgg caatatcttc tccgttgcgc actttgcgtc cggcaccgat gctgatcttt
     1321 ttgaaaggtg ttttccagtt caccattacc ccttcaccgc gcgggtaacg gatactccat
     1381 gaccccttgc actcttcgag ttgggcggtg tacatcagat cgcgcagttc ttcttcgttc
     1441 atgggagagg ccacgatcat attgggaata caacgcatgt aagcgagatc gtacgcaccg
     1501 tgatgcgtgg caccgtctgc accaaccaat ccaccgcgat cgagacagaa aatcacatgc
     1561 agtttttgca atgcaacatc gtgaatcacc tgatcgtagg cgcgctgcat gaaggaggag
     1621 taaatattgc agtagggaat caatccctgc gtggctaatc ctgccgaaaa agtgacggca
     1681 tgctgttctg caatccctac atcaaatgcg cggtcgggca tggctttcat catgatgttg
     1741 agcgaacatc cggaaggcat tgcaggagtt accccaacaa ttttatcgtt gcgctcggcc
     1801 aattccacaa tgctgtgacc aaacacatcc tggtacttgg gtggctgggg cgatttgggg
     1861 gcgatcttga tgatctctcc ggtatccttg ttgaacactc cgggtgcatg ccaaacggtg
     1921 gcgttgcctt cttcggaatg cttgtagcct tttcctttta cggttacgca atgcagaatc
     1981 ttcgggccgg gaatgtcttt gaggtccttc atgatgcggg tcaggttgat cacgtcgtga
     2041 ccatcgaccg gaccaaaata gcggaagttg agcgactcga acagattgct ttgtttgagc
     2101 agtgagccct tgatcgaatc ggaaattttt tgagcgatgg cctgggcgtt ggggccgaac
     2161 ttgctcactt tccccagcag gttccagact tcgtctttga ccttgttata cgtatgcgac
     2221 gtagtgatat cggttaagta ctccttgagc gcccccacat tcgggtcgat ggacatacag
     2281 ttatcgttga gaaccaccag aagattactg ttagtaacac ctccatgatt cagaccctca
     2341 aaggctaagc ctgctgtcat ggctccatcg ccgattactg caatgtgctg gagatcttcc
     2401 ttgccgaggt acttattggc cacggccatt cccactgcgg cggagatgga agtggatgaa
     2461 tgtccaaccc cgaaggtatc gtattcgctc tcgctgcgtt tggggaaacc tgagagtccc
     2521 ttataggtcc ggttggttgg gaaagcatcg cgtcggccgg ttaggatctt gtgtccgtaa
     2581 gcctggtgtc cgacatccca caccaactga tcgttgggag tattgaaagc atagtgcagg
     2641 gccacggaaa gctccacaac gccgagactg gcaccgaagt gtgaattacc gatatgggac
     2701 accacatcga tgatgtaatc gcggagttcc tgggagattt gcgggaggtc ctcctcctgg
     2761 aatttactgc gcaaatcgag cggtgaatca atctggctta aaagcggacc gggtttaaaa
     2821 tcgggcattg ttgggggttt ggagaaggga gaacaaagat agggcttttt tgttcggggt
     2881 ggcatagaaa cgacacccgc ttaagtgtag tatctgatcc agttcctatt taggattttt
     2941 taaatattca gcaacacctc agccaatttc aaatcgaagg acgttgtgat cttgatgttt
     3001 tcgcggttgc cttctacaag gttgatgtga acacctagtt tctctactac actggcatcg
     3061 tcggtgaagg aaggataata gtcctgcgca taggcacgat ggatcagttc cgcatcaaag
     3121 cactgtggcg tttgtaccaa gcgataatgc gaacgatcaa cgggacgggt actttcgcca
     3181 aacacctcgc gcaccgaatc caccaccggt aataccggaa tggcattgcc tttctctgca
     3241 gcagtttcga gagcgtaacg aatggtgtgt ttgcttacca atgggcgcac cccgtcgtgg
     3301 atggctacca atcccgattt cggcacctcc tttaaggcat tcttcaccga aaaaaagcgt
     3361 tctgctcctc cggcaacgag tgtttgctcc accggaatct ttttcattgc cgcatgattc
     3421 ttccagtagt cgatgtactc ttcgggcaaa accaccacat agcgaaaagt gggatccact
     3481 ttgtgaaaag cctcaatggt atgatgcaaa atcgccttgc cgtggatggg gaggaattgc
     3541 ttaggctgtg cagaggccat gcgtttcccg gagcctccgg ctacgatgat gatggttgtg
     3601 ggggaaggga ggtttttcac cactaagacg cttagggcac taaggtacgc caagaatagc
     3661 tttaacaaaa attagggagg ccgaaagacc tccctaattc aaaaatttcg tgttttaata
     3721 agggggcttc gactccccga agttgatggt cacttagtcc ctttctccct ttgtggtttc
     3781 attaaaaaag gcactttatc cattaacggt ctcaattaaa taatcaacat agcatcacca
     3841 taggaataga atcggtattc ttccttgatg gcttcttcgt atgctttcat tacaaaatca
     3901 taaccgccaa aggcagtaac catcatcaac aatgtgctca ttggcatatg gaagttggtg
     3961 atcatgctgt ttgcaatgga gaaatcgtat ggcgggaaaa tgaatttgtt cgtccagcca
     4021 tcgaagggct tgaggaaacc gtcggttgaa acggatgttt cgattccacg cattacggtt
     4081 gttccaacag cacatatacg tttacggtcg gctttggcca tgttaaccgt atccgcgcaa
     4141 agagcaggaa taattacctg ctcagattcc attttatgct tggtaagatc ttctacttct
     4201 actgtgcgga aggtgcccag accaacatgc agcgttagct ctgcgaaatt tacgcctttg
     4261 agttcaagac gcttcatcag ttccttggag aaatggagtc ctgcagtagg agctgcaact
     4321 gcaccttcat gttgtgcgaa aaccgtctga tagcgatctg catcttcctt ttccacttcg
     4381 cgtttgatgt atttcggaat tggagtttct ccgaggcgct caatggtttt tttaaattct
     4441 tcgtacggac catcaaacaa gaaacgaagt gtgcgtccgc gtgaagttgt attgtcgatt
     4501 acctcagcaa ccagcgaatc atcgtcgccg aagtacagtt tatttccgat acggatttta
     4561 cgtgcaggat ctaccaacac atcccagagg aggctctcac ggttcaattc gcgaagcaga
     4621 aatacttcga tttttgctcc ggttttttcc ttgttaccgt acatgcgcgc aggaaacact
     4681 ttggtattgt tgagaatcat cacatcacca tctccaaaat attccatgat gtcgcggaac
     4741 ttgcggtgtt cgatggtctg cgtctcacga ttcaacacca ttaaacggct ttcgtcgcgg
     4801 ttggcagcag gatggttagc gatgagttcg gggggaagtt taaacttaaa ttgactgagt
     4861 ttcatgcgtt taattaagtt actattcgat tgaaggggcg caaagataga ctgtgcctac
     4921 cccctttgtc aagcatttgg gagaatatat ttcgaaatca ccgggcgaag caattaagag
     4981 gccttcaggc gagagcgata ttgattacct ttgtgctctg aaaattacat ccttgaatac
     5041 cgctaaacgc cacaccatta ctgccgcatt gccctacgca aacggtccct tgcacatagg
     5101 tcacattgca ggagcatatc ttcctgccga catatacgtg cgttacctca aaatgcgtgg
     5161 cgaagacgtg gcctttattt gcggaagcga tgaacacggt gcagcgatta caatccgtgc
     5221 gaaaaaagaa ggtattagtc cgagggagat catcgacaag taccattcca tcaacaaaaa
     5281 agcatttgcc gatttgggta ttgcattcga tatttttcac cgtacgagtg atccattgca
     5341 tcatcaaacc gcacaggatt ttttcattca gctggaaaaa aacggggctt ttactaaaga
     5401 aacatctgag cagtttttcg atgaagagca tcagcagttt ttagccgatc gttacataac
     5461 cggaacctgt ccgaagtgcg gaaacgaaaa cgcatacggt gatcagtgcg agaaatgtgg
     5521 ttcgtcgctg aatccgacgg atcttatcaa cccaaaaagc acgcttagcg gaaaagcacc
     5581 tgttctgcgc aaaaccgaac actggtattt acccatggag cgtcatgaag catggatcag
     5641 agagtggatc gaaaaaggaa cgctcgacgg aaagccgcat catgatccga aagaatggaa
     5701 aacgcatgtt gtgggtcagt gcaaaagctg gatcgatgcc ggtttgcaac cacgcgccat
     5761 gacacgcgat ctcgactggg gagtaaaagt tccggttgaa ggcgcagccg gaaaagtgct
     5821 ctacgtttgg ctcgatgcac ccatcggata catcagtgcc accaagcaat gggcaaaaga
     5881 caaaggaaaa aactgggaag actactggaa atcgcccgat acccgtttgt tgcatttcat
     5941 tgggaaagac aacatcgttt tccattgcat catcttcccg atcttgctta aggcacacgg
     6001 cgaatttttg ttacctgaga atgttcctgc caatgagttt ttgaatctcg aaggcgataa
     6061 actatccacc tcccgcaact gggcagtatg ggtacacgaa tacctcgaag aacttcccgg
     6121 caaggaagat gaattgcgtt acgttctagc atccattgcg cccgaaacaa aagacagcga
     6181 attcacctgg aaggattatc aggcaagagt caacaacgag ctcgttgcga tctttggaaa
     6241 ttttgtgaat cgggtggtag ttttaatgga gaaatattat cagggaattg ttccagaaat
     6301 aaacggctcc atcaaaaccg aacaggagaa aaaattattc ggagagttaa ttccctcaca
     6361 ttggaaatcg cttgaggaca acatgaatcg gtatcgtttc cgtgaatccc ttgccgctgc
     6421 gatggacatt gcccgtgaag gaaataaata cctcgccgaa acagaaccct ggaagctcat
     6481 caaatcggat actgaacgca ccaaggaaat tcttttcaat gcattacaga tatgtgcaca
     6541 attgtcggtt gcatttgagc ccttcctacc caacaccagc aacaaactgc gtggcctgtt
     6601 aaacattgct cctattgatt ggaaaaacgc aataaatgga aatcttgttt cttccggaca
     6661 tactctcggt aaaccaggac ttctcttcga aaaaatcgac gatattgtga ttgaaaaaca
     6721 gatgttgaaa ctggaagaaa gtcgcaaagt ctacaaggaa ctcacagcac cttcttcaag
     6781 tacggtgaag acccaaaaat cagatatcac cttcgatgaa ttttcgaaga tggatattcg
     6841 tgtaggcact attttggctg cagaaaaagt ggaaaaggcg gataaattgc tcaaactaac
     6901 tgtcgatact ggaattgacc agcgcatgat tgtttccgga attgccgaac attatacacc
     6961 ggaggaagtg attggtaaaa aagtaagcgt cctcatcaac cttgctcccc gtaaaatcag
     7021 aggtgtagaa tcgaaaggaa tgattctgat ggcagaaaac gctgaaggaa agctttgttt
     7081 tatttcgcct gtaaatacgt tcgactcagg gtcggaaatc agataattcg atttctaaac
     7141 tgattttgcc ttaatattga ttcatgagtc aagcagctga atccacgctt ccctattatc
     7201 cttttgaagt acgcgtttac gatgctgaaa ttatcattgc tgagttggtt caggaaattg
     7261 aggctgtaga aatcgaacac atgcacgcac ttacaagagc acttgaagaa atgggtgagg
     7321 gtaaaaagtg gaaagtggtt acagtgatcc acaattacag ccttttggat accaaagcaa
     7381 gagaatacgc ctcaagtgaa tacgcacaac ggtttaccat tgcaaatgcg attgttgtca
     7441 cctcgcttgc cacacgaatt ggcgcgaatt tttttatcaa tttttccaaa ccgcctcgcc
     7501 caacc
//
LOCUS       DAOMYG010000107         7497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3303_length_7497_cov_8.925961, whole genome shotgun sequence.
ACCESSION   DAOMYG010000107 DAOMYG010000000
VERSION     DAOMYG010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7497
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3303_length_7497_cov_8.925961"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PK610_04795"
     CDS             complement(<1..580)
                     /locus_tag="PK610_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35280.1"
                     /translation="MFKKLVLTIVPVLLLNLNSWAQPDMSAYGVINPSSGLGLLSNES
                     VVLEYYNYGSDIQSGDSLLFSYSINNGPWVNETKILSALFPNFGYATYVFTTEADLSV
                     PNNYEVKVVVSHLSDTYRYNDTIVKTVIHAASPMLAFVSPFEVTQDQSNYPTIHGIGT
                     DFDTYELVYFIRGNDTIASPIWRTLAAGVMLSC"
     gene            complement(661..1152)
                     /locus_tag="PK610_04800"
     CDS             complement(661..1152)
                     /locus_tag="PK610_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35281.1"
                     /translation="MPVTRDLTKLKTVSISEYRKPKTLTEIETQEDGTPIQGPMDDFS
                     AEQFDQAWTEIANKMKAQGKDSIVVCLTKRKPELASDFKIMLTIDNKTMENQLEREKQ
                     DILDFIRQRLNNSAIIIETRMAEEGKEQAFLYTNKDKFKKLAEKNPHLILLQKRLNLD
                     IEF"
     gene            complement(1299..2498)
                     /locus_tag="PK610_04805"
     CDS             complement(1299..2498)
                     /locus_tag="PK610_04805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRJ35282.1"
                     /translation="MNTVLKLYTVQASGRFGSLYLHNAFRHMDNFIVSARKYRPQTFD
                     TVVGQSAITSTLKNAIASKHLAQAFLFCGPRGVGKTTCARILAKTINCMDLQPNTEAC
                     DKCESCQSFNDGRSLNIYELDAASNNGVDAIRSLIEQVSFAPQLGQYKVYIIDEVHML
                     STGAFNAFLKTLEEPPRHAIFILATTEKHKIIPTILSRCQIFDFNRIRLQDIADHLQK
                     IALSEGITAEPEALHVIAQKADGGLRDALSIFDQVVSFSGNNLTYNAVIDNLNILDYD
                     YYFRVTDAIFGKDIKNVLLIYDEILKKGFDGHIFINGLASHFRDLLVCKDPQTLILLE
                     TSENVKSKYQSQSQAAPAPLLIQALELYSKCDVQYKSSKNQRLLVELTLMQACSLDPQ
                     AEKKNLN"
     gene            2596..2958
                     /gene="rpsF"
                     /locus_tag="PK610_04810"
     CDS             2596..2958
                     /gene="rpsF"
                     /locus_tag="PK610_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRJ35283.1"
                     /translation="MSNRYETVFILTPVLSDDQAKEAVAKFKGILKDLKAKVVHEEFW
                     GLKKLAYPIQKKSTGFYQLFEYEADSQAVARLEVEFRRDERVLRFLTIKLDKHSIAYN
                     EKKRGGEFKSKKKEKAEA"
     gene            2985..3251
                     /gene="rpsR"
                     /locus_tag="PK610_04815"
     CDS             2985..3251
                     /gene="rpsR"
                     /locus_tag="PK610_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRJ35284.1"
                     /translation="MASDNDIRYLTPISIETTKEKYCRFKKSGIKYIDYKDPNFLLKF
                     VNEQGKILPRRVTGTSLKFQRKVSQAVKRSRHLALMPYVADMLK"
     gene            3272..3718
                     /gene="rplI"
                     /locus_tag="PK610_04820"
     CDS             3272..3718
                     /gene="rplI"
                     /locus_tag="PK610_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRJ35285.1"
                     /translation="MEVILKKDIEKLGYKDDVVKVKDGYGRNYLIPQGLAIVATGSAK
                     KVLSENLKQRAHKEAKVRQEAEKLGKALATVSVKVGAKAGENGKIFGSVNTIQVSDAL
                     KKLGYDVDRKHISIKNEPIKVLGKYEAVIRLHKDLTETITFEVVEE"
     gene            3816..5111
                     /locus_tag="PK610_04825"
     CDS             3816..5111
                     /locus_tag="PK610_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HRJ35286.1"
                     /translation="MKNTLQRYTDSPFIRNVFTLAAGSGLAQLISVGISPVLSRLFTT
                     DDFAVYAVFFSWMLVIAIAAALRLDTAIPLPASESEAIGLSRLAIKIALLVTLICLIV
                     VVVLLLIYSDDAEKSIPGFYILLPITMLTTVLMQVYNYLSTRWGSFRFNATSRVLTNI
                     VIAGVSVVLGYLQWGPLGLILGLLIGQTAGVAFMYFSMRKRIRETDIPEQRERPILLL
                     KKYSDFIWINTPHALLDTLQLAATIWILDFYFEDKVVGWFFLSWRILKMPLTFIGSAV
                     FQVFYSKASKEFNAGKNIQHSIGKIYGQMFLLGLPICLVLLAFGPALFAFVFGEEWRG
                     AGEMSRILSLWLFANFVVSPVSCIAMIAGKQKQAFLLAIIEHGSRLGLLAVGGWFGDL
                     ELALWLTTISGVIMMLVMMYWYRSIADGSKSRRVVMDEL"
     gene            5260..6105
                     /locus_tag="PK610_04830"
     CDS             5260..6105
                     /locus_tag="PK610_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ35287.1"
                     /translation="MSQHRIIIEPGRENNNYWRDIRKYRGLFYFLAWRDVLVRYKQTV
                     IGVAWSVMRPLLTIVVFTFVFHNLGNFSAGESIPYAIMVCAGMLPWQFFSGAFSEAAN
                     SLVANSNLLTKVYFPRLIVPASTVIVSLIDFFISLVILGGLCVYYQFVPDWRIVFLPL
                     FLMLAIVTAMGAGLFIAALNVKYRDFRYIIPFIVQFGLYISPVGFSSELIFGNESVPQ
                     YLKVIYALNPMVGVIDGFRWSILGDGTVLHWMEFGASCGISVLLLFTGVWYFRRMEKS
                     FADVI"
     gene            6107..7354
                     /locus_tag="PK610_04835"
     CDS             6107..7354
                     /locus_tag="PK610_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ35288.1"
                     /translation="MAAIITVENLSKSYMIRHEGRERYVALRDVVSNKAKSLFSGKRE
                     LTSSAYEEFKALDDVSFTIEQGDRVAIVGRNGAGKSTLLKILSRITSPTKGRIILDGR
                     VASLLEVGTGFHPELSGRENIFLNGSILGMSKAEIKSKFDEIVAFSEVERFLDTPVKR
                     YSSGMYVRLAFAVAAHLEPEILVVDEVLAVGDAAFQKKCIGKMQDVSKSGRTVLFVSH
                     NMSAVRNLCNKAILLQNGKLIVQDEVEPVLERYLASNQTGQLTGNYTRPDKKQSDVML
                     SSAAVVDAQGEERNAFEIIEQVGLKLNLTCNKPVPNLYGYVVVKNSNEEVIIESDTKD
                     LPPNALDGITEGNTTFLLTIPANTLSVGEYTVYLSFSSSFAADFSVETPGDVLRFAIS
                     DSITERGHNRRSYTSVLLNWQKK"
BASE COUNT         2016 a         1444 c         1753 g         2284 t
ORIGIN      
        1 agcaactcaa catcaccccg gccgccaggg tccgccagat cgggctggca atggtgtcat
       61 ttcctctgat aaaataaaca agttcataag tatcgaaatc tgttccgata ccatgaatgg
      121 tcgggtaatt ggattgatcc tgtgttactt cgaaaggtga tacaaatgca agcatcggac
      181 tcgcagcatg tatcacagtc tttacaattg tatcgttata acggtaagta tcacttaaat
      241 gactcacaac aacttttact tcgtaattgt tgggtacgct taagtcggct tctgttgtga
      301 atacataagt ggcataacca aaattcggaa acaatgccga taggatcttg gtctcattta
      361 cccaaggtcc gttattgatg gaataactaa aaagtaagct gtcgcctgac tgaatatcag
      421 aaccataatt gtaatattcc aatacgactg attcgttact taataatccc aatcctgaac
      481 ttggatttat aacgccgtat gccgacatat ccggttgggc ccaagaatta aggttaagta
      541 gtaaaacagg aacaatagta agtacaagtt ttttgaacat gttggataat tttgagcaaa
      601 tctaaaccaa ttaagcgaaa taagaccttg attggtagga taaaatgaag gaatggtcgt
      661 ttaaaactct atatcgaggt tgagtcgctt ttgcaagagg atcaaatgcg ggtttttttc
      721 cgctagtttt ttgaatttat ccttgttggt gtacaaaaag gcttgttctt ttccttcttc
      781 agccattcgg gtttcaatta ttatggctga attattcagc cgctgccgaa taaaatcaag
      841 gatatcctgt ttttctcttt caagctgatt ttccatcgtt ttattgtcga tggttaacat
      901 gattttgaag tcggaagcga gctccggctt gcgtttggtc aggcaaacaa caatggaatc
      961 cttgccctgg gctttcattt tattggcgat ttctgtccag gcctgatcga actgttcagc
     1021 actgaaatca tccattgggc cctgaatagg agtaccgtcc tcctgggttt caatttcagt
     1081 tagggttttg ggtttacggt attcgctgat cgaaactgtt ttcagtttgg taagatcgcg
     1141 cgtaactggc atggtttttc cgccggattc gaccggtgct tcgggtgcag aaggaagttt
     1201 tattgcagtt gcagaaggag cggctgagcg gctgagtcgg gtttgtgcct gaaggtaatt
     1261 gggattgaca tgtccgccct ccggaggaag gattacgatc agttcaggtt ttttttttca
     1321 gcctgaggat cgagggagca cgcttgcatg agtgtgagtt caaccagtaa ccgctggttt
     1381 tttgaactct tgtattgcac atcgcatttc gagtacaact ccagcgcctg aatcagtaat
     1441 ggagctggtg cggcttgtga ctgcgattga tattttgatt ttacgttttc agaggtctcc
     1501 agtaaaatca aagtttgtgg atctttgcaa acgagtaagt cacggaaatg tgaagcgagt
     1561 ccgttgatga aaatatgacc atcaaatcct ttcttgagaa tctcatcata gatgagcaaa
     1621 acgtttttaa tgtctttgcc aaaaatggca tcggttaccc tgaaataata atcgtaatcg
     1681 agaatgttga ggttgtcgat taccgcattg taggtgagat tgtttcctga aaagctgaca
     1741 acctggtcga aaatggataa ggcatcgcgc aaaccgccgt ctgctttttg agcaataaca
     1801 tgtaaagcct caggttctgc ggttattcct tcgcttagtg caattttttg gagatgatca
     1861 gcaatatcct gcaaacgaat ccggttgaaa tcgaagattt gacaacgcga aagaatggtt
     1921 ggtataatct tgtgtttctc ggtggtggca agaataaaaa tagcgtgacg tggtggttct
     1981 tcgagtgttt tgaggaacgc gttaaatgct cctgtactca gcatgtgcac ctcgtcgatg
     2041 atatacactt tgtactgacc aagttgagga gcaaagctga cctgctcaat aagcgagcgg
     2101 atagcgtcta caccattgtt cgacgcagca tccagttcat aaatattgag tgaacgacca
     2161 tcattaaacg attggcagga ttcgcattta tcacaagctt ccgtgttggg ttgcaaatcc
     2221 atgcaattga tggtctttgc cagaattcgt gcacaggtgg ttttaccaac cccgcgagga
     2281 ccacaaaaca aaaatgcctg ggcaagatgt ttgcttgcaa tggcattctt caaagtagag
     2341 gtaatggccg actgacctac aaccgtatca aaggtttgcg ggcggtattt tcgtgccgat
     2401 acaatgaagt tatccatgtg cctgaatgcg ttgtgcaaat ataagctgcc aaatcgccct
     2461 gatgcctgta ctgtgtataa ctttaggact gtgttcattt tcctcctttt tcacttgttc
     2521 cgataatcct ttttgctatc tttgccgtcc atttccgaat ggaagatttg taaaccatct
     2581 caaaatcaat aatttatgtc taaccggtac gaaactgttt tcattttaac tcccgttttg
     2641 tctgatgatc aggcgaagga agcagttgct aagttcaagg gtatccttaa ggatctcaaa
     2701 gccaaggttg tgcatgaaga gttctgggga ctgaaaaaac tggcttatcc aatccagaag
     2761 aagtccacag gtttttacca gctcttcgag tacgaagctg acagtcaggc agtagcccgt
     2821 ctggaagttg aattccgtcg cgatgaacgt gtgctacgct ttctgaccat caagctcgac
     2881 aaacattcga tcgcttacaa cgagaagaag cgcggcgggg aattcaaatc caaaaagaaa
     2941 gaaaaggctg aagcctgatg tccaacctca tttaaacaaa gattatggca agtgataatg
     3001 atatccggta cttaaccccc atcagtattg aaacgacaaa agaaaaatat tgtcgcttca
     3061 agaaatcggg catcaagtac atcgattaca aagaccccaa cttcttattg aagttcgtta
     3121 acgagcaggg aaaaattctt ccgcgtcgtg taacaggtac atctttaaaa ttccagcgca
     3181 aagtatcgca ggcagttaag cgttcacgtc acctcgcttt aatgccctac gttgctgata
     3241 tgctgaaata aaccatttaa cgaattgcgt catggaagtt attcttaaga aagacataga
     3301 aaagctcggc tacaaagacg atgttgtgaa ggtaaaggac ggttacggac gtaactacct
     3361 tatcccacaa ggtcttgcta ttgttgcaac cgggtcggct aagaaagtac ttagcgaaaa
     3421 ccttaagcag cgtgcccata aagaggcaaa agtgcgccag gaagctgaaa agcttggtaa
     3481 agcactcgct actgtatcag taaaagttgg agccaaagcc ggtgagaacg gaaagatctt
     3541 cggatctgtt aacaccattc aggtttctga tgcactgaaa aaactcggct acgatgttga
     3601 tcgcaaacat atcagcatca agaatgagcc tatcaaagtt ctaggaaagt acgaagcggt
     3661 tatccgtctt cacaaagacc ttactgagac catcactttt gaggtggtgg aagagtaaat
     3721 cgaacaattc cttcagcccc ggcttattgt cggggctttt tttttgcacc ccggcacgaa
     3781 tttcaggttt tgcaggttat tgaacggaac accttgtgaa aaatacctta caacgttata
     3841 cggactcacc attcattcgc aatgtattta cacttgcggc aggatctggt cttgcccaat
     3901 tgatttctgt tggtatttcg cccgtccttt cccgtttatt cacaaccgat gattttgccg
     3961 tatatgcagt gtttttttcc tggatgctgg taattgccat tgctgcagct ttgcgtttgg
     4021 atactgccat tcctcttcct gcgagtgaat cggaggcgat tggtctttcc cgcctggcca
     4081 taaaaattgc gttgctggtg acactcattt gcctcattgt ggtggtggtt ctgctgctaa
     4141 tctattcgga tgatgcagaa aagtcgattc ccggatttta cattttgctc ccgattacta
     4201 tgctcaccac agtgctgatg caggtgtaca attatttgtc gacccgctgg ggcagctttc
     4261 gtttcaatgc aacatcgcgg gtacttacca atattgtgat tgcgggtgtt tcggtggttc
     4321 tgggttacct gcaatggggt ccgcttggtt tgattcttgg tctgttgatc ggacaaactg
     4381 ccggtgttgc atttatgtat ttctccatgc ggaaacgcat acgcgaaacg gatattccgg
     4441 aacaaaggga gcgtccgatt ttactcctga aaaaatacag cgattttatc tggatcaaca
     4501 ctccgcatgc gctattggat acgcttcaac tcgcggccac catctggatt ctcgattttt
     4561 actttgaaga taaagtagtg ggctggtttt ttctgtcctg gagaatatta aaaatgccac
     4621 tcacatttat tggttctgca gtattccagg tgttttattc aaaggcctca aaagagttca
     4681 atgccggaaa aaatatccag cattccattg gtaagattta tggacaaatg tttttgttgg
     4741 gcttgccgat ttgtttggta cttcttgcat tcggacctgc actttttgcc tttgtgtttg
     4801 gcgaagaatg gagaggcgca ggagaaatgt cgagaattct ttcgctgtgg ttgttcgcca
     4861 attttgtggt gtcgccggtt tcctgcattg caatgattgc cggaaaacaa aagcaggctt
     4921 ttttgctggc aataattgaa catggctcgc gattgggatt attggcagta gggggttggt
     4981 ttggtgacct ggaactagcc ttgtggctga ctaccatttc gggtgttatt atgatgctgg
     5041 tgatgatgta ttggtacagg agtatagccg atggatcaaa aagcagacgt gttgtaatgg
     5101 atgaattata aggggattat taatgatttc aatgcgttat tgagtaattg gactgcattc
     5161 gtaaacaggt tctgaagttt gttcactctt ccatctaaac accagtcctt tacttttacc
     5221 tatctttgat aagtccttga agaaggacca acattcagca tgagtcagca tcgcatcatt
     5281 atcgaacccg gaagggagaa taataattac tggagagata tcaggaaata ccggggactc
     5341 ttttattttc ttgcttggcg cgatgtattg gtacgttaca aacaaacagt gattggagtt
     5401 gcatggagtg tgatgcgtcc tttattgacc attgttgttt tcacgtttgt cttccataac
     5461 cttgggaatt tctctgccgg agaaagtatt ccctatgcga ttatggtttg tgcaggaatg
     5521 cttccatggc agtttttttc cggtgcattc agtgaggctg caaattcatt ggtagccaat
     5581 tcaaacctcc tgacaaaagt atatttcccc cgattgattg ttccggccag tacggtaatt
     5641 gtaagtttga tcgatttttt catttccctt gtcattcttg gcggactatg tgtttattat
     5701 cagtttgtgc ccgactggcg aattgtattt ttgcccttat ttctaatgct ggctatagta
     5761 actgcgatgg gtgcaggttt gtttattgct gcccttaatg ttaaataccg cgatttcaga
     5821 tacatcattc catttatagt gcagtttgga ttgtatatct ccccggttgg atttagcagc
     5881 gaacttattt ttggaaatga aagtgtacct caatacctta aagtgatata tgcactaaat
     5941 cctatggtag gtgtaattga tggttttcgc tggagtattc ttggtgatgg aacggtcttg
     6001 cattggatgg agttcggagc ctcatgcgga ataagtgttt tgcttttatt cacaggagtt
     6061 tggtatttca ggcgcatgga gaaatcattt gccgatgtaa tttgatatgg ctgcaataat
     6121 cacagtagaa aatttgagca agagctacat gattcgtcat gaaggacggg agcggtatgt
     6181 ggcactcaga gatgtggtga gcaataaagc gaaatctctt ttttcgggga agcgtgaatt
     6241 gacttcttct gcctatgaag aatttaaagc gctggatgat gtttcattta caatagagca
     6301 aggtgaccgc gttgcaattg tcggacgaaa tggtgctggc aaatctactt tgttaaaaat
     6361 actatccaga attacatcac caaccaaagg aagaataata ttggatggtc gtgttgcaag
     6421 tttacttgaa gtaggtacgg ggtttcatcc tgaattgtcc ggaagagaaa atatcttttt
     6481 gaatggttca atcctgggga tgagcaaggc agaaatcaaa agtaagtttg atgagattgt
     6541 tgcattttca gaagttgaac gctttttaga tactcccgtt aagcgttatt cttccggtat
     6601 gtatgttcgt ctcgctttcg ctgtagcagc ccatcttgaa cctgaaattt tggttgtgga
     6661 tgaggtgctg gctgtgggag atgctgcgtt tcagaaaaaa tgcattggta aaatgcagga
     6721 cgtaagtaaa tcaggacgca ctgttctgtt tgtgagtcac aatatgtcgg cggtgcgtaa
     6781 tctttgcaat aaggcaattc tgctgcagaa cggaaaattg attgttcagg acgaagtgga
     6841 acctgtattg gagcggtatc ttgcgtcgaa tcaaaccgga cagcttaccg gtaattatac
     6901 gcgtcctgat aaaaagcaaa gtgatgtaat gttaagttcc gctgcagttg tggatgcaca
     6961 gggtgaagaa aggaatgcgt ttgagattat tgaacaggtt ggtttgaaac tgaatcttac
     7021 ctgcaataaa cctgttccca atttgtacgg atacgttgtg gtaaagaaca gcaatgaaga
     7081 agtaatcatt gaaagtgata ccaaagatct tcctcctaat gcattggatg gaattacaga
     7141 aggcaatacc acatttcttc ttactattcc tgcaaacacc ttgagtgtag gagaatacac
     7201 tgtttatctt tcattttcct ccagttttgc tgctgatttt tcagtagaga cacccggtga
     7261 tgttttacgt ttcgctatta gtgattccat tacggaaaga ggacataacc gccgatctta
     7321 taccagtgta ttattaaact ggcagaagaa atgagtcttc gcgattttat ccctcccgta
     7381 atttttaagg tgcgttcagc gttgatcaat gcggtgaaag gaaatgggtt aaatacagag
     7441 gagaaagcat tgctggagcg gaattcagta ttcaaaaaca aacataccgg aaaacga
//
LOCUS       DAOMYG010000108         7466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3319_length_7466_cov_11.389421, whole genome shotgun sequence.
ACCESSION   DAOMYG010000108 DAOMYG010000000
VERSION     DAOMYG010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3319_length_7466_cov_11.389421"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..3417
                     /locus_tag="PK610_04840"
     CDS             46..3417
                     /locus_tag="PK610_04840"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HRJ35289.1"
                     /translation="MKNRLLFAFFVFLSSFSFGQITITPGPTGAALTSSFMGNGLTAT
                     NVVINCNAQSYGTFANGNTTNLGLTNGLLMTTGLATQAPVIGGSSSGNDASFCVGTSV
                     NDPQLTSIEPSATNDVCIIEFDVTAVCNQLQVRFVFGSEEYPEYVNAGVNDAFGFFVS
                     GPNPGGGNYTNFNIARLPNNSIVSIDNVNAGVNSAFYVSNSGTTIEYDAFTTIITYSL
                     NLTPCANYHFKLAIGDAGDCIIDSGVFIDFIACTTPPINVTTASTAASCGANDGTATA
                     TVTGGAGPFNYSWNTTPVQTTATATGLAPGNYTVTVTSTAFPCLPPSTSTVTVGGSGA
                     APTVTVTPTTGTICNGGNVSLTAAGGTSYTWSPATGLSSTTGATVTASPTTTTTYTVT
                     GTGACGTGTASATITVNPVPTTTAGSNSPLCVGATLNLTATNSTGATYSWTGPGGFTS
                     TQQNPSLPNVTTGNAGTYTVTVSANGCTSTSTVNVAVNSTFNATITPAGPFCVNDPAI
                     TLLAVDAGGSWSGNGITNAATGIFTPSVAGAGTHTITYTIPGACGAAANTSITVNPAV
                     SAAITPVGPFCVGDPAITLTGATPGGTWSGNGITNASTGTFDPATAGQGTHTITYTIG
                     GACGDSQTTTITVNTTSNATITPAGPFCVNNANAFLAAVTPGGSWSGNGIVNASTGEF
                     SPTAAGAGTHTITYTISGSCGDVQTTTIQVIANANATVNPAGPFCVSDPATTLTSVNP
                     GGTWSGNGITSAANGTFDPSVAGNGTHTITYTISGVCGDAQSITINVVSILPTTITPV
                     NPICINASPITLSGASPGGTWSGTGITNTTTGIFDPAVAGAGTHTITYTIAGNCGSTS
                     TTNITVNPLPIISFNVDINSGCVPVTSTFTNTTPGASNCNWLIDGVPASTSCSMFTHT
                     FTQVNCFDITLVTTDNNGCSNTATINDMVCTFPIPNADFTFTPTNTTVLNPTVNFTNQ
                     STGGTIYQWDFAGLGTSTQTNPSFTFPDSAASYLVCLNVSNASGCSDDICKVVTIYDE
                     LLIYVPNAFTPDGDGKNDVFLPIVAGIDPNSYEFFIFNRWGELIFESQVPDRPWDGTY
                     RSQNSKQDVYVWKIKAKKQGSGEKVVYYGHVSLLR"
     gene            complement(3483..4412)
                     /locus_tag="PK610_04845"
     CDS             complement(3483..4412)
                     /locus_tag="PK610_04845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ35290.1"
                     /translation="MSSEQEYRIVHRKEIDADKWDALVADSSQGMIYHMSFYLDAICE
                     NWSAVMNVSGDIALPFAWKRRYGYFNIYQPFFSRYQAFLHRPDVAPDLIPILKTLPEA
                     HDMHLCLPQTLPAYPEGTLAEKWVYQLKKLPEQHSELPGTYSENTRRMIRKATDAGLR
                     MCDVQPDELIRFFRKEKGADIAEFRDDDYSRLLQLLLAGKKNNKLKVVGVTNQQELVA
                     VACFWFFKDRITYLKGTTSEKGRSTGAMFYLFDQVILQSSSEYKIFDFGGSRIPSIAT
                     FYHRFGAIDQDYFSFTIGRPPAHVRMLRKLLRK"
     gene            complement(4402..5697)
                     /locus_tag="PK610_04850"
     CDS             complement(4402..5697)
                     /locus_tag="PK610_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ35291.1"
                     /translation="MDNKIRKASVLIIIVAALGYFVDIYDLVLFGVVKTESLEQIMPG
                     ATAAARSDMGKFLFNMQMLGMLIGGVLWGVLGDKKGRLKVLFGSILLYSAANIVNAFI
                     TTIPAYTVVRIIAGIGLAGELGAGITLVSEMMPKEKRGYGTMIIVTFGALGAVVASLV
                     GAKGEAIGSLISSVTGSPIANWQVAYIVGGVMGLALLLLRIGTIESGMFTGIRSESKI
                     AKGDIRILLFNSQNLKKYLRCIFVGIPIWYVIGLLVMNSENEFAPFLGVEGVKNGKAV
                     MYAYIGLSVGDLISGLLSQLLRSRRKVVMLYLLLTLILIFVFLFFSHDISLQGYYILC
                     LLLGTASGYWAIFVTIAAEQFGTNIRSTAANTIPNFVRGSVNIITGLFGMLTAFAVPK
                     DVNALLVGLLFITLAFYSIVKLKETFSKDLDYVEDHHEL"
     gene            5785..6432
                     /locus_tag="PK610_04855"
     CDS             5785..6432
                     /locus_tag="PK610_04855"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HRJ35292.1"
                     /translation="MTLKPCFLRRRRTTCESWNTAIYTRKLGKSITLFADATLRFVTR
                     PWQWRQQLVRVAPEIKVLPKLYLGFGYAFSNQHVIDRPSNHSFEHRPHFQLVFQQNNE
                     KAIHRLRLRTEGRFIQSNSSGSDTDYFTRIRFQYRGAFPRKKNSSQHFVLAEETMLQA
                     NGINDFGFNQQRLYIGFRMNKEKTRAWEIAYMNILRVPNNSDIDLTHTLWINLFF"
     gene            6432..6728
                     /locus_tag="PK610_04860"
     CDS             6432..6728
                     /locus_tag="PK610_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35293.1"
                     /translation="MKALRTSLFILLTIAFVTPEYSCRSMAKAAAKHWTKRQVKKFKK
                     NCKSKLNGHLPEAKVDAFCGCATDKISQAFDLNKANQMDHIALLKEAASCLLNK"
     gene            6876..7145
                     /locus_tag="PK610_04865"
     CDS             6876..7145
                     /locus_tag="PK610_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35294.1"
                     /translation="MASRKDLKQLINSFCYDVIDDCLTLIELQKAGEEKIRPLLNDAI
                     ELRNDLMLRFGQARKAENPGAVYKAIYTDLENNTLEYIDRLNALV"
     gene            7185..>7466
                     /locus_tag="PK610_04870"
     CDS             7185..>7466
                     /locus_tag="PK610_04870"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRJ35295.1"
                     /translation="MFPKAHILFFLLVLPFTNGKAQDIHFSQFDLSPINLNPGNTGLF
                     DGTYRFAGNFRNQWRSVTVPYSTFSLSADANNFLEKKGLGAGIRINHDIA"
BASE COUNT         2273 a         1767 c         1423 g         2003 t
ORIGIN      
        1 aaaaataccc catcttactg ttgcactacc tgaaaccacc ccattatgaa aaatcgcctg
       61 ctgtttgcat tttttgtgtt tctatcttcc ttttcattcg gacaaatcac cataacaccc
      121 ggtcctaccg gtgctgcgct gacgtcctcc tttatgggga acggacttac ggcaaccaat
      181 gttgtgatca actgtaatgc acagtcttac ggaacatttg ccaatggaaa cacgaccaat
      241 ttaggcttaa ccaacggctt acttatgaca acaggtttgg cgacccaagc tccggtaatt
      301 ggtggtagct cctctggaaa tgatgccagt ttttgtgttg gaaccagtgt aaatgatccg
      361 caactgacat ctattgaacc aagcgcaaca aacgatgttt gtattatcga gtttgatgtt
      421 accgctgttt gtaatcagct acaggtgcga tttgtttttg gctcagaaga atatccggaa
      481 tatgtaaatg ccggtgtaaa tgatgccttc ggtttttttg tatcaggacc aaatcccggt
      541 ggtggtaatt acaccaattt caacattgca cgactcccca ataattcgat tgttagtatc
      601 gacaatgtaa acgcaggggt aaattcggca ttttacgttt ccaattcagg tactacaatt
      661 gaatacgatg cattcaccac gataattacc tattcactta atttaactcc atgtgccaac
      721 tatcatttca aattggcaat aggagacgca ggcgattgca ttatcgactc cggtgtgttt
      781 attgatttta ttgcctgtac aactccccca ataaatgtca caactgcatc aacggctgca
      841 tcatgcggtg caaatgacgg aacagcaact gccactgtaa caggaggagc cggaccgttt
      901 aactacagtt ggaatactac tccagtacaa accactgcaa ctgctaccgg tttagcaccc
      961 ggaaattata cagtcactgt tacctcaaca gcatttcctt gtcttcctcc atcaacttca
     1021 actgttactg ttggaggatc aggtgccgct ccaactgtaa ccgttactcc aacaacagga
     1081 acaatttgta acggaggaaa cgtgagcctg actgctgcag gaggaactag ttatacctgg
     1141 tctccagcaa ccggattgag ctccaccaca ggagcaacgg ttactgcttc tccaaccaca
     1201 acaaccacct atacggtaac cggaactggt gcatgtggta caggaactgc cagcgcaaca
     1261 ataacagtta atcctgtccc tacaaccact gcgggatcca attctcccct atgtgttggg
     1321 gcaacgttaa accttaccgc aacaaactca accggagcca cgtattcgtg gacaggtccg
     1381 ggtggattta catccacaca acaaaacccc tccttaccta acgttacaac aggaaatgca
     1441 ggaacatata ccgtaactgt atcggccaac ggatgtacat ccacttcaac tgtgaacgtt
     1501 gccgtaaact ctacttttaa tgccaccatt acacccgccg gaccgttctg cgtgaatgat
     1561 cctgcaatta ctttattggc agttgacgct ggaggatcct ggagcgggaa cggaattacc
     1621 aatgcagcca cgggaatatt tacaccttca gttgccggag caggaacaca taccatcacc
     1681 tatacaatac caggagcctg tggtgctgcc gcaaacacaa gtattacggt taatcctgcg
     1741 gtaagtgcag ctattacacc tgttggtcca ttttgcgttg gtgatccggc tatcaccctt
     1801 actggagcaa ctcccggcgg aacatggagc gggaatggta ttaccaatgc atccaccgga
     1861 accttcgatc cggcaacagc cggtcagggt acacatacga tcacttatac cattggaggc
     1921 gcatgcggtg actcgcaaac aacgactatt acagtcaata caacatccaa tgccacaatt
     1981 acacctgcag gacctttctg cgtaaacaat gcgaatgcat ttctcgctgc tgtaacaccc
     2041 ggtggatcat ggagcggtaa cggaattgtt aatgcctcaa ccggagaatt cagtccaact
     2101 gctgcgggtg caggaacgca taccataacc tataccatta gcggaagttg tggtgatgta
     2161 caaaccacaa ccattcaggt aatcgcaaat gcgaatgcaa ctgtaaatcc tgcaggaccg
     2221 ttctgcgtat ctgatccggc aacaacatta acctcggtaa atcccggagg cacatggagt
     2281 ggaaatggaa ttacaagcgc tgccaacgga acctttgatc cttctgttgc aggaaacgga
     2341 acacatacaa ttacctatac aataagcggt gtttgcgggg atgcacaatc cattacaatt
     2401 aatgtcgttt ccattttacc aacaacaatt actcccgtaa atccgatttg tattaatgct
     2461 tcacctataa cattaagtgg agcatctcca ggcggtacat ggagtgggac cggaattaca
     2521 aatacaacta cagggatttt tgatccagcc gttgcaggag ccggaacaca tacaattacc
     2581 tataccattg ccggaaactg cggaagcaca agtacaacaa acattacagt aaatccactg
     2641 ccaatcatca gctttaatgt tgatatcaat tcaggttgcg ttccggtaac cagcacattt
     2701 accaatacaa ctccgggagc aagcaattgt aattggttaa tcgatggtgt tcccgctagc
     2761 acctcttgtt cgatgttcac acatacattt acacaggtaa attgcttcga catcacactg
     2821 gtgactacgg acaataacgg atgttccaac actgcaacta taaacgatat ggtttgtact
     2881 ttcccaatac cgaacgccga tttcacattt acacctacaa atacaaccgt gttgaatcca
     2941 acagtaaact tcaccaatca atctacagga ggtacaattt accaatggga ttttgcagga
     3001 ctgggaacat caacgcaaac aaatccgtcc ttcaccttcc ccgacagtgc tgcatcctat
     3061 cttgtgtgcc tgaatgtatc caacgcatca ggatgttcgg atgacatttg taaagttgtt
     3121 actatttacg atgagcttct gatttatgta ccaaatgcct tcacgccgga tggagacgga
     3181 aagaatgatg ttttccttcc aatcgtagca ggtattgatc caaactccta tgaattcttc
     3241 atctttaatc gttggggtga attgattttc gaatctcagg tacctgatcg tccatgggac
     3301 ggaacctatc gctcacagaa ctccaaacaa gatgtttatg tctggaaaat caaagcgaaa
     3361 aaacagggaa gtggagaaaa agttgtgtat tatggtcacg tatccttatt gagataaaca
     3421 aatcccaaat attcaattgc ggagtctgag ctgggcttcg gatctgctca gactccgcta
     3481 atttactttc tcagtaattt cctcaacatg cgcacatgag caggaggtct gccgattgtg
     3541 aatgaaaaat aatcctgatc tattgcaccg aaacgatggt agaatgttgc gatagaggga
     3601 atacgagatc ctccgaaatc gaaaatttta tattctgaag aactctgcaa tattacctga
     3661 tcaaaaagat aaaacatcgc accagtgctt cttccttttt ctgatgtagt acctttgaga
     3721 taagttatac ggtctttaaa aaaccaaaaa caagctactg caacaagctc ctgctggttg
     3781 gtaactccca caacttttag cttgttgttt ttctttcctg caagcaacaa ttgaagcaaa
     3841 cgcgaatagt catcatctct gaattctgca atatcagcgc ctttctcttt tcgaaaaaac
     3901 cgaatgagtt catccggctg aacatcgcac attcgaagtc cggcatctgt cgctttgcga
     3961 atcattcttc gcgtattctc agagtatgta cccggcaatt cgctatgttg ctcaggtaac
     4021 tttttcaatt gatacaccca tttttcagcc aaagtccctt caggataagc cggcaatgtt
     4081 tggggaagac acaaatgcat atcgtgtgct tccggtaagg ttttgagaat gggaattaga
     4141 tccggagcaa catcaggacg atgaagaaat gcctgatacc ttgaaaaaaa aggctgataa
     4201 atattaaaat aaccgtagcg tcttttccag gcaaatggca aggcaatatc cccggagaca
     4261 ttcattactg ccgaccaatt ttcacaaatt gcatcgagat aaaaactcat gtgataaatc
     4321 attccctgag atgagtcagc taccaatgcg tcccatttat cagcatcaat ttccttgcgg
     4381 tgaacaatgc ggtattcctg ctcagagctc atgatgatcc tccacgtaat ccaggtcctt
     4441 tgaaaacgtt tccttaagtt ttacaatgga ataaaatgca agggttataa aaagcaatcc
     4501 taccagaagt gcatttacat ctttaggaac agcaaatgca gtgagcattc cgaataatcc
     4561 ggtaataata tttaccgaac cacgaacaaa attgggaatg gtattcgctg ctgttgaacg
     4621 aatatttgtc ccaaactgct ctgctgcaat ggtaacaaat atggcccagt atcctgaagc
     4681 tgttcccaat aacaaacaaa gaatataata tccctgcaga gaaatatcat gtgagaaaaa
     4741 cagaaataca aaaatcaaaa tcagtgtcaa caacaaataa agcattacca ctttccttct
     4801 gctgcgaagt agttgactta ataatcctga aatcaggtca cccaccgata atccgatata
     4861 agcatacatc actgccttcc cgtttttcac accttcaaca cccagaaatg gagcaaattc
     4921 gttttcagaa ttcataacaa gaagtccaat tacataccag atcgggattc ccacaaaaat
     4981 acaccggagg tactttttca gattttgaga attaaataac aaaatgcgaa tatccccttt
     5041 tgcaatttta gattctgagc gaattcctgt aaacatcccc gattcaatgg tgccaatccg
     5101 aagcagcaat aaagcgagtc ccatcactcc accaacgata taagcaacct gccaattggc
     5161 aatcggacta ccggtaaccg agctaatcaa acttccaatt gcctcacctt ttgcaccgac
     5221 caaagaagca actaccgcac ccaacgcacc aaaagttaca atgatcattg taccgtaacc
     5281 tcttttttcc tttggcatca tttctgaaac aagtgtgata cctgccccga gttcaccggc
     5341 aagaccaatt cctgcaataa tccgaacaac cgtataagca ggaattgttg tgatgaatgc
     5401 atttacaatg ttggcagcag agtacagtaa aattgatcca aacaaaacct ttagtcgtcc
     5461 ctttttatcc ccgagcacac cccacaaaac cccaccaatc agcattccca gcatctgcat
     5521 attaaacaga aatttcccca tatcgcttcg ggcagcggca gtggctcccg gcatgatctg
     5581 ttcaagactt tctgttttaa ccacaccaaa gagtaccaga tcatagatat ctacaaagta
     5641 accgagtgca gctacaatga tgataagaac tgaggctttc cggattttgt tgtccatgtg
     5701 atcgtgagga atataacgtg aaaatacaaa aggactgatt attattgtaa ttgaaatgcg
     5761 atttcctgct cttcttttag ttttatcact cttaagccct gcttccttcg caggcgtaga
     5821 acaacatgtg aaagctggaa cacggctatt tacacccgga aacttggaaa atccattacg
     5881 ctttttgcag atgcaaccct acgatttgtt accagaccat ggcaatggcg acaacaattg
     5941 gttcgggtag ctcccgaaat caaagtttta ccgaaattgt atttgggatt cggttacgct
     6001 ttttccaacc aacatgttat tgacagacca tccaatcact ctttcgaaca tcgtccgcac
     6061 tttcaattgg tttttcagca gaataatgaa aaagcaattc atcgtcttcg cttaagaact
     6121 gaaggccgat tcatacaatc aaattcttca ggcagcgata cggattactt caccagaatc
     6181 agatttcaat accgaggtgc cttcccgcgt aaaaaaaact catctcaaca ttttgttttg
     6241 gcagaagaaa ccatgcttca ggcaaacgga ataaacgatt ttggctttaa ccagcagcga
     6301 ttatacatcg gttttcgcat gaacaaggaa aaaacaagag cctgggaaat tgcctacatg
     6361 aatattctcc gtgtcccgaa taattccgat attgacctga cacataccct ctggatcaat
     6421 ttatttttct gatgaaagca ctacgcacct ccctttttat tctgctaacc atagcattcg
     6481 ttacaccaga atactcgtgt cgatcaatgg caaaagcagc agccaagcat tggaccaaac
     6541 gtcaggtaaa aaaattcaaa aagaattgca aatctaaact caacggacat ttaccggaag
     6601 cgaaagtgga tgcattctgt ggatgtgcca ctgacaaaat cagtcaggcc ttcgatctga
     6661 acaaagccaa tcaaatggat catattgcac tcctcaagga ggcggcttca tgtttattga
     6721 ataaataata ctttctggat tgaaagcaca cattattttt taatagaaaa aaagtgctgt
     6781 tttcatttta taaagattct cccttcattt caggaagcga atcagagaaa gaacatatat
     6841 aaatcatttg actatctttg tctcaaacta attatatggc atcgcgtaaa gaccttaaac
     6901 aactgatcaa ttcattctgt tatgatgtga ttgacgattg tcttacacta atcgaacttc
     6961 aaaaggcagg cgaagaaaaa atccgtccct tattgaatga tgccattgaa ttacgcaacg
     7021 atttaatgct ccgttttggt caggcaagaa aggctgagaa tcccggtgct gtttacaagg
     7081 ctatttatac cgacctggaa aacaacactc tggagtatat agacaggctc aacgcgcttg
     7141 tgtaagcact tgcgctgaat cgaacttttt gctattttta ggatatgttc ccaaaggcac
     7201 atatcctgtt ttttctgctt gtacttccat tcaccaatgg caaggctcag gacattcatt
     7261 tttcgcagtt tgatttatcg ccaataaatc taaatcccgg taatacaggt ctgtttgatg
     7321 gcacctaccg attcgccggt aattttcgca accaatggag aagtgttact gtaccttact
     7381 ccaccttttc actttctgct gacgcaaata attttttaga gaaaaaagga ttgggcgccg
     7441 gcataaggat caatcacgac attgca
//
LOCUS       DAOMYG010000109         7445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3329_length_7445_cov_9.496346, whole genome shotgun sequence.
ACCESSION   DAOMYG010000109 DAOMYG010000000
VERSION     DAOMYG010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7445
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3329_length_7445_cov_9.496346"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1773
                     /locus_tag="PK610_04875"
     CDS             <1..1773
                     /locus_tag="PK610_04875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ35296.1"
                     /translation="SIAQTGPGGVGANDGSSSLKLWFRGDNGVSVSGTMINSISNSAG
                     IAALDISESGANRPTLAAGAVNGYNEIVFSGTTMLRSGLTLTTTNFVTNQASSFTVSR
                     AANTTQTSCVYTTDPLVGTTRFSNHIPWSNTIYFDIGTCCSNDARLQVGSLTGLNNYS
                     IWTYDAEPTSGKQLYRDGTLLQDRPNTLAYNSHASHRFNIGGNTSGTNGFVGAVTEII
                     IFNAKINSAQRIIIDNYLSAKYNISLAANDVYLMDDPANGNYDHEVAGIGRINATNTH
                     TDARGTSIVRISSPSNLGNNEFLMWGHDNGTMTTNNTVDVDGTTIQSRLSRVWRASEF
                     TGDVGTVTIAFDVSGFTTVTGSDLRLIIDRDGDGFFDNDVAPISGAFAANTITFTGVN
                     LQNGDRFTLGSINVSQTPLPVELLLFNAIREGQDVRVRWSTASESNSDHFKVERSVNG
                     TTWTEIAHVHAQGNSVVQQNYFSLDLQPVFGMNYYRLKMVDQDASVNYSIIVPVDFSD
                     AVATAVSVFVNSFNGEVSLISENVPQQMQGIHIYDISGKIVFSHSFESTQLARIPTEQ
                     LSAGTYVIIVTSKDKSYSSKFIRY"
     gene            complement(1775..3415)
                     /locus_tag="PK610_04880"
     CDS             complement(1775..3415)
                     /locus_tag="PK610_04880"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRJ35297.1"
                     /translation="MKRITLLLFFLFSLINTQAAQPKLIVGLVIDQMRYDYLVRFASK
                     YSKNGFARLMNQGIHCTNAHYGYVPTYTGPGHASIYTGTTPSVHGIIANDWFDRKEGA
                     MVYCSEDKNVRTIGSDSKAGEMSPKRMQTSTIGDELKLFTNGKSKVIGLALKDRGAIL
                     PAGHSADGAYWFDDASGNFITSSFYRNDLPQWLIDFNEKKRTNHFLSQVWNTLLPIAA
                     YTESLPDNNTYEFVAKSETEPVFPHNLPEIVKANENRLSIIKSTPFGNTLTKELMFAA
                     IEGEQLGKDNTTDLLCVSFSSPDYIGHAYGPRSVETQDCYLRLDLEIAEILNYLDKNI
                     GKGQYLLFLSADHAASDNPNHLKSMRIPSGYLDLSKYKDSLNNYLLEFFSLNDPSERF
                     VRAIVNEQVYLNTDLIRRKGFDKEAIEQKTAEFMRNIQGVGNVITRSDMLRTCYQHGV
                     GKLLQAGFNELRSGDVIISLLPAWMDYGPKGTTHGTAYSYDTHVPILIHGQGILPQRI
                     TSRIDITQIAPGLCRYLDIPFPNGCFSDPIPEMLKGKK"
     gene            complement(3480..4139)
                     /locus_tag="PK610_04885"
     CDS             complement(3480..4139)
                     /locus_tag="PK610_04885"
                     /inference="COORDINATES: protein motif:HMM:NF021576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HRJ35298.1"
                     /translation="MKILPTSLKGWTGVLIIIAFSIMMFSISLPYFAMDKKADIDFLK
                     QKLNVYHIDSWRIGFYTHVFTSVLLLIAGITQFSSRIIKGFPRLHRRMGMTYLIVLIF
                     FSGPGAFVMGIYANGGPAARVSFVFLTLIWMAFTIFAWYYVRKKDYIAHGEFMIRSYA
                     LTLSAITLRLYLYFIHHFHFGLKPLDKYIIVAWLSWVPNLIIAEILIRREMAAKLMKK
                     Q"
     gene            complement(4139..4894)
                     /locus_tag="PK610_04890"
     CDS             complement(4139..4894)
                     /locus_tag="PK610_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35299.1"
                     /translation="MNRIILISLGLLMITSCGTSSEKSDQVNDNSTTTSNAKKYDTRQ
                     LEGMYMGQFGKATLMLQINYVKGKNASGFNILRGNRRNIKGTVENKGDRYFFILDEPG
                     NDKYDGKFEFEIDTTQYNLVGKWIPNDPKSAQLREFTLKRVEQKDADPNIVGDWQAGL
                     WSVHGIMTFKKDGTLVFDGYNYNEESSESENVRVNGTWLEDGEIIRLEIGKHPLLKKQ
                     LIELKKASEEGFGDMFLLKSGEDVTLYPYGMYF"
     gene            complement(4964..>7445)
                     /locus_tag="PK610_04895"
     CDS             complement(4964..>7445)
                     /locus_tag="PK610_04895"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HRJ35300.1"
                     /translation="LGFVNSNFAGVSGIDLQPASVVDNRLKTDVSLFGFNVSAYNNYI
                     GLKPGALVNNGSMFKPDMPAFNDTAFQANYLSERYNDIPKAIYFSTQLQLPSFMFQID
                     RKSAFAFTWRVRTMMNVDGLSKELARLAYNGLDYPSLWNTFHTSQNVSIQAMSWAEYG
                     ATYGRVLMDKGDHFLKAALRVKVLQGLGAAYMYMDNVQFQALNSDTIQITSNGRVGYG
                     HSTNFELDNESVKYKFISNFSMGFDLGAVYEYRPKIKDYLYDMDGEKELRRRDQNKYK
                     LRAGLSVIDVGAIRYKKGIRSNDFNTNAALWDLSALKFDTLRPIQSFDDTLINRFGTA
                     SGAKDYFSMNLPTAISVQVDYNIWKDFYINHTTYLAFQFKNNENKVHDISTFSLTPRY
                     DSKWFGLFVPVSYNLMGNFAVGTGVRLGPVIFGTTNLSPIIQYMKGDGSGKKDTYGMD
                     FHMVMKIPIPYGKPKDKDGDNVSDKKDKCQGERGIWEFAGCADRDGDHIQDSKDDCPD
                     EPGLPQFNGCPDKDGDGIIDKNDHCPDEAGTLEFNGCPDRDGDKIIDKEDECPDQAGP
                     IAFKGCPDTDNDGIPDPKDDCPNDRGLSEFNGCPDTDSDGVRDIEDKCPLIPGPILNF
                     GCPDKKLQKLTLAGSMLEEVTMEDGKFNFKQDIDKKAHKFRLLGTATDTISEVYVTGP
                     NLRGKFAKKNGSFFQFPQEAEAVELTPEEQAVVKKAFDNLEFATGKDIIKPESLASLD
                     ELAQLMKKYPGWKLKIEGHTDNVGVRAKNVELSKKRAEAVKKYISSKGISPTRFEVKW
                     YGPDRPVAPNDTEEGRQKNRRVEMTIVE"
BASE COUNT         2109 a         1522 c         1509 g         2305 t
ORIGIN      
        1 tcgatcgcac aaactggtcc cggtggtgtt ggcgcgaatg atggatcttc gagtcttaaa
       61 ttatggttcc ggggagataa cggtgtttct gtttccggta caatgatcaa ctcgatctct
      121 aattcagcag gtattgcagc tctagatatt tctgaatcgg gagctaatcg acctacattg
      181 gcagcgggag ctgtaaacgg gtataatgag atcgtttttt caggaaccac tatgcttagg
      241 agtgggttga ccttaacaac gacaaatttt gttacgaacc aagcaagtag ctttaccgtg
      301 agcagggctg caaatactac gcaaaccagt tgtgtttata ccacagatcc attagtagga
      361 actactcgtt tttcaaatca cattccttgg tcaaacacaa tttattttga tattggaaca
      421 tgctgttcga acgacgccag gttgcaagtt ggaagtttaa cagggttaaa taattattca
      481 atttggacgt atgatgcaga accaacatcc ggaaagcaat tgtataggga tggaacgctt
      541 ttgcaggatc ggccaaatac acttgcctac aatagtcatg cttctcatag gtttaatatt
      601 ggtggtaaca catcaggtac aaatggtttt gtcggggcgg ttacagagat aattatattt
      661 aatgctaaaa ttaattcggc gcagcgaatt ataatcgata attatctttc tgcgaaatac
      721 aatatttcgc tagcagcaaa tgatgtttat ttaatggatg atcctgctaa tggtaattat
      781 gatcatgaag tagcaggaat tgggaggata aatgctacca acacacatac tgatgcaaga
      841 gggacttcca tagtgcgcat cagctcacct tccaatcttg gtaataatga atttttgatg
      901 tggggtcatg ataacggtac aatgaccaca aataatacgg tagatgttga tggaacaaca
      961 attcaatcgc ggttgtcaag ggtttggcgg gcaagtgaat ttaccggtga tgttggtaca
     1021 gttacaattg catttgacgt ttcaggattt acaactgtta ccgggagtga tcttcgttta
     1081 atcattgatc gcgacggaga cggatttttt gacaacgatg tggctccaat ttccggagct
     1141 tttgcagcga acaccattac ttttaccggt gttaacttac aaaacggtga tcgctttact
     1201 ttaggctcaa ttaatgtcag tcaaacgcct cttcctgttg aattgctttt gtttaacgct
     1261 ataagggaag gtcaggatgt tcgcgttcgc tggagcacag caagtgaatc gaatagcgat
     1321 cattttaagg tagaacgatc agtgaatgga acaacctgga ctgaaattgc acatgttcat
     1381 gctcagggca attcggttgt acaacagaat tatttttcac ttgatcttca accggttttc
     1441 ggtatgaatt attaccgatt gaaaatggta gatcaggatg caagtgttaa ttactcgata
     1501 attgttccgg ttgatttttc agatgcagtt gcaacagcag tttctgtttt cgtaaattct
     1561 tttaatggag aagtaagtct gatctcggaa aacgttccac aacaaatgca gggaattcac
     1621 atttacgaca tctccggaaa aattgttttt tcacattcgt ttgaatctac acaacttgcc
     1681 cgaatcccaa cagagcaatt atctgcgggt acttatgtca ttattgtaac tagtaaagac
     1741 aagagttatt cctcaaaatt catccgatac tgatctactt ttttccttta agcatttccg
     1801 gtatcggatc cgaaaaacat ccattcggaa aaggaatatc gagataacgg caaagccccg
     1861 gagcaatctg cgtgatatcg attctgcttg taattctttg aggaagtatt ccctgaccgt
     1921 gaattaaaat agggacatgc gtatcataac tataagcagt accgtgcgtg gttcctttcg
     1981 gtccataatc catccatgcg ggtaataaac tgatgatgac atcacccgaa cgtaattcgt
     2041 tgaagccggc ctgcaataat tttccaaccc catgctggta acatgtgcga agcatgtcac
     2101 tacgtgtaat tacatttcct acaccttgaa tattgcgcat gaactctgct gttttttgtt
     2161 caatagcctc cttgtcgaat ccttttctgc gaatgagatc ggtattcaaa taaacctgtt
     2221 cgttaacaat ggcgcgtaca aaacgttctg agggatcatt cagcgaaaaa aattccagca
     2281 aataattatt gagtgaatct ttgtacttcg acagatcaag atagcctgat ggaatgcgca
     2341 tgcttttgag atgattcgga ttatcgctcg ccgcatgatc agcactaaga aagagcaggt
     2401 attgaccttt accaatattt ttatcgaggt aatttaaaat ctctgcaatc tccagatcca
     2461 atcgtaaata gcaatcctgt gtttctactg aacgcggacc ataagcgtga ccaatgtaat
     2521 ccggacttga aaaacttaca cagagaaggt cggttgtatt atcttttccc agctgttcac
     2581 cctcaatggc agcgaacatt aattcttttg taagtgtatt tccaaatgga gttgatttaa
     2641 taatactcaa tctgttctca ttggctttta caatttcagg caagttatgt gggaaaacag
     2701 gttctgtttc actcttggca acaaactcgt aggtgttgtt gtcgggtaat gattctgtat
     2761 atgcggcaat gggtaatagt gtattccaaa cctgagaaag gaaatgattt gttcgttttt
     2821 tttcgttgaa atcaatcagc cattgcggaa gatcatttct gtaaaatgaa cttgttataa
     2881 aattaccgct tgcatcatca aaccaatatg caccatccgc cgaatgtccg gcaggtaaaa
     2941 ttgctcctct gtcttttaat gccaatccga ttactttcga cttaccattg gtaaataatt
     3001 taagttcatc gccaatagtt gaagtttgca tgcgtttggg cgacatttct ccggctttgg
     3061 agtcgctgcc aattgttctt acatttttat cttccgaaca ataaaccatt gctccttctt
     3121 ttcgatcgaa ccagtcattg gcaatgattc catgtactga aggtgtggtt ccggtgtaaa
     3181 tggatgcatg tcccggtccg gtataggttg gtacgtatcc gtaatgtgca tttgtgcagt
     3241 ggattccctg attcattaaa cgtgcaaatc cgtttttgct gtatttggat gcaaagcgca
     3301 caagataatc gtaacgcatc tggtcgataa ccagtcccac aattaatttg ggttgtgcag
     3361 cctgtgtgtt gatcagacta aaaaggaaga ataaaagaag tgtgatacgt ttcatggtaa
     3421 gaatcttgtt gggtaaaaat agggacttca tattcagcct gtgtgaaaaa tcggtgaaat
     3481 cactgttttt tcatcagttt ggcggccatt tctctgcgaa tgaggatctc agctatgatg
     3541 agattgggaa cccagctcag ccatgcaaca atgatgtatt tatcgagtgg ttttaatccg
     3601 aaatgaaaat gatgaatgaa ataaagatat aaacgtaatg ttatagccga taaagtaagt
     3661 gcgtagctgc ggatcataaa ttcaccgtga gcgatataat cctttttcct gacataatac
     3721 catgcaaaaa ttgtaaaggc catccagatc agggtaagaa atacaaaact tactcttgca
     3781 gccggacctc cattagcgta tattcccatt acaaacgctc cgggaccgct aaaaaaaatc
     3841 aatacaatta ggtaagtcat tcccattctt ctgtgcaagc gtggaaatcc tttaatgatg
     3901 cgtgaactga attgtgtaat tcctgcgatg agtaataata cactggtaaa aacgtgtgtg
     3961 tagaaaccaa tacgccaaga atcgatatgg taaacattga gtttctgctt tagaaaatca
     4021 atatctgctt tcttatccat tgcaaaataa ggcaacgaga tcgaaaacat cattatgcta
     4081 aatgcaataa taatcaatac gcctgtccaa ccctttaaac ttgtgggaag aattttcatc
     4141 agaaatacat tccgtaggga tacagcgtta catcttcacc actcttgaga aggaacatat
     4201 cgccaaaacc ttcttcactg gcttttttca attcgataag ttgctttttc aaaagcggat
     4261 gtttcccgat ctcaaggcgg attatttcac cgtcttccag ccaagttccg ttaaccctta
     4321 cattttcact ttcagagctt tcttcattat agttgtaacc gtcaaaaacc aatgtcccgt
     4381 cttttttaaa tgtcataatt ccgtgtaccg accacaatcc tgcctgccag tcgccaacga
     4441 tgttcggatc ggcatctttt tgttcaacgc gctttaaagt gaattcccgc aattgagctg
     4501 attttggatc attgggtatc cacttgccaa caagattgta ttgagttgta tcaatttcaa
     4561 attcaaattt gccgtcgtat ttgtcatttc cgggttcatc aagaatgaaa aagtaacgat
     4621 cccccttgtt ctcaacggta cctttaatgt tacgacgatt tccccgcaga atattaaatc
     4681 cggatgcgtt ttttcctttt acataattga tctgcaacat caatgtagcc tttccaaatt
     4741 gacccatata cattccttca agctgacgcg tgtcgtattt ttttgcgttt gaagttgtag
     4801 tggaattatc attgacttga tcactttttt cggaagatgt tccgcatgag gtgatcatca
     4861 aaagaccgag actgattaga atgattctgt tcatgaaaat agaattagga aacagcagaa
     4921 aggtaacaaa aaaagtcccg acttgtgggt cgggactttt caattattcg acgatggtca
     4981 tctctacgcg tctgtttttc tgacgacctt cttcagtgtc attcggagca acaggacgat
     5041 cgggtccgta ccatttcact tcaaagcgtg tagggctgat gcctttggat gaaatgtatt
     5101 tcttaacggc ctccgcacgt tttttagaaa gctcaacatt cttggcgcga acacccacat
     5161 tgtcggtgtg accttcgatt tttagtttcc atccgggata tttcttcatc aactgcgcaa
     5221 gttcatcaag tgaagcaagc gattcaggtt tgataatatc ttttccagta gcgaattcaa
     5281 gattatcgaa tgcttttttc accacagcct gctcttcagg tgtaagttca actgcttctg
     5341 cttcctgagg gaactggaag aaagatccat ttttcttagc gaatttacca cgaagattag
     5401 gtcctgttac gtacacttca ctgatagtat cagttgcagt tcccaacaag cggaacttat
     5461 gcgctttctt gtcaatatcc tgtttaaagt tgaatttacc gtcttccata gtaacttctt
     5521 caagcatact accagcaagg gtaagtttct gaagtttttt atcaggacaa ccaaagttca
     5581 ggattggccc gggaataagc ggacatttgt cttcgatatc acgaacgcca tcactatctg
     5641 tatccggaca tccgttgaac tcgcttagac cacggtcgtt cggacaatca tctttcggat
     5701 caggtatgcc gtcgttatca gtatccggac atcctttgaa tgcaatcggt cctgcctgat
     5761 ctgggcattc atcttctttg tcaatgatct tatcaccatc acgatccgga catccgttga
     5821 attcgagtgt acctgcttca tccggacagt ggtcattttt atcgatgatt ccatcgccgt
     5881 ctttatcagg acaaccattg aattgtggta atcccggttc atccggacaa tcgtctttcg
     5941 aatcctgaat gtggtcgcca tctctgtcgg cacaacctgc aaattcccaa ataccacgtt
     6001 ctccctgaca cttatctttt ttgtcggata cattatctcc gtccttgtct ttcggtttac
     6061 cgtaagggat aggaattttc atcaccatat ggaaatccat tccatacgta tcttttttcc
     6121 ctgagccatc acctttcata tactgaatga tggggcttaa attggttgtt ccaaaaatca
     6181 caggaccaag acgaactcct gttccaacag caaaattccc catcaggttg tagcttaccg
     6241 gaacaaatag accaaaccat tttgaatcgt atcggggagt aagtgagaaa gtagaaatat
     6301 cgtgaacttt attttcgttg tttttaaatt ggaaagcaag ataggtggtg tgattgatgt
     6361 agaaatcttt ccagatgttg tagtcaacct gtactgagat tgcagttggg aggttcatcg
     6421 agaaataatc ttttgcacca cttgcagttc caaatcggtt gatcagggta tcgtcgaatg
     6481 attgtattgg acggagcgta tcgaacttaa gtgcactcaa atcccaaagc gctgcattgg
     6541 tattaaaatc attggaacga attccttttt tataccgaat tgcccctaca tcaattacag
     6601 aaagtcctgc gcgtaatttg tatttattct ggtctcttct gcgcaattct ttttcgccat
     6661 ccatatcgta gaggtaatct ttgattttgg gacggtattc gtaaactgcg ccaagatcga
     6721 agcccatgga gaagtttgaa atgaatttat acttcacact ttcgttgtca agttcaaagt
     6781 tggtggagtg accataacca actctgccat tacttgtaat ctgaatcgta tctgaattta
     6841 atgcctgaaa ctgcacattg tccatataca tgtatgcagc acctaaacct tgtaatactt
     6901 ttacacgaag agctgctttt aaaaagtgat cgcctttgtc catcagaacg cgtccatatg
     6961 ttgcaccgta ttcagcccag ctcatggcct ggatggaaac attttgtgag gtgtggaagg
     7021 tattccataa tgaggggtaa tccaatccat tataggcaag gcgagcaagc tctttgctta
     7081 aaccatcaac attcatcatt gtgcgtactc tccatgtaaa agcaaaagca gatttgcggt
     7141 cgatctgaaa cataaatgaa ggcaattgca attgcgtgct gaaatatata gcctttggta
     7201 tatcattata acgctcactt agataattcg cctgaaatgc agtatcgtta aatgcaggca
     7261 tatccggttt gaacatgctt ccgttgttta ctaaagcacc cggctttaat ccaatataat
     7321 tgttataggc agaaacatta aagccaaaca aggaaacatc tgtttttagt cggttgtcca
     7381 ccacagatgc gggttgcagg tcgattccgc tgacacctgc gaaattactg ttgacaaagc
     7441 caagg
//
LOCUS       DAOMYG010000110         7417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3345_length_7417_cov_7.861043, whole genome shotgun sequence.
ACCESSION   DAOMYG010000110 DAOMYG010000000
VERSION     DAOMYG010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7417
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3345_length_7417_cov_7.861043"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PK610_04900"
     CDS             <1..700
                     /locus_tag="PK610_04900"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ35301.1"
                     /translation="YCHADESTFQQADQLYKSQQYDSARVLFESMLAEDKQSAIVFFA
                     LGNCYYRLSDPVTAVYYYEKAIKLDPNNPDLKANIAFVRKQFKDKVNTDRSGISGWVF
                     SIVNDNHPNKWSGTAVILINFAFLSLILSRFLSAKKSRIAFIAGSVALVLGISRLIPA
                     WYQYSSITNHDQGIVKTDRTEIKSAPSENSSTVFILSEGTKVNITESNEFWLEVNIGD
                     ANVGWIQREQILEI"
     gene            697..1488
                     /locus_tag="PK610_04905"
     CDS             697..1488
                     /locus_tag="PK610_04905"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HRJ35302.1"
                     /translation="MSRTKPSIVILGTGNVAYALGIALFKNGYPIKQVYSRSHEKALQ
                     LARKLKASFTDDLRAIEIDASVYLLAISDDALPEFCKALPRLGGIVMHTSGSIPLSVL
                     KKQNHTAVIYPLQTLQKDHQPEWQKTSFLVEANDEVGLKMVKSIARTLTKKVIQCTSE
                     ERKQVHLAAVIASNFSNHLFALSQDILQSNKLPTDLLAPLIEETIKKSLKGDAKTKQT
                     GPARRGDQTILRKHLNMLNNDPLTKSIYKQISASIQKMYSKKSKK"
     gene            1485..1898
                     /locus_tag="PK610_04910"
     CDS             1485..1898
                     /locus_tag="PK610_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRJ35303.1"
                     /translation="MNYWLLKSEPDVFSWDDLVKKGPSVWDGVRNYQARNNLKAMKKG
                     DKALFYHSNIGREIVGIATITKPFFPDPTIDDERWVAVEVKAQKKFKNAVTLDTIKNT
                     PALAKLPLVTHSRLSVMPVSELDFVLLCDMGGIVS"
     gene            1982..2689
                     /locus_tag="PK610_04915"
     CDS             1982..2689
                     /locus_tag="PK610_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010520413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRJ35304.1"
                     /translation="MGRAFEYRKARKMARWSAMSTTFTKIGKEISMSVKAGGSDPNTN
                     SRLRAAMQNARSANMPKANVEAAIKKATDKDASNYEEVNYEGYGPNGVAIFIETATDN
                     TTRTVANMRTIFNKGGGSIGTSGSLDFVFERKANFTVKNTGINVEELELELIDAGLEE
                     IEADAEEIFIVTRFADFGAMQKALEGKGFEILSAEKVRTPLSTVEVTPEQREAIDKMV
                     EKFEEDDDVQQVFTNMA"
     gene            2914..>7417
                     /locus_tag="PK610_04920"
     CDS             2914..>7417
                     /locus_tag="PK610_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35305.1"
                     /translation="MIPLPLFRMTGILLLAFYSVISNAQTTYTWNGITSTDWNTSTNW
                     TPNGTPSPTDHVVIVTGSNQCTLPGNVDVTNFSMTSGTLNVGANTLTVTGTFSALNGT
                     ITGTGNLFANGSSVVFGNTIAGPTVSPNVSISTLSVRSQRTTYHGNVTILKTIGATAV
                     DSWRGGNTFNGTFYLNNASDDIAGNNGDIYFGSNNGDPVDVFNGRTTMIVSGAARIRI
                     PQNGSTIFNGVTHFLSNGFGGQHDRIQPARQGAANAVFNDSVYFTCASATSDMHIAYD
                     AGTSCVFNGPVVCNRLTGSGGAFNLGLDGTITLNSNLIVNNNSTGSITVTSGSGSSTL
                     ANGNSIFVGAEGFNSGSLDIRNFTQIGNTTQNLVFSSAAALFIGAAGSPCTFNANTIN
                     FSAGRVRSQSTTYNSTSISFSKTGATADDNGDNFFGGPVTFSNSGGAEFRFSSTIARD
                     VFASTVTLVNSGTGMISMSRTFDTDYDFNIEINNPSVTAGGGIQFGANGGSSTLASGR
                     TISVGGSGYEFGYLYLFRFNQLGGTPQTITLGANSTLLRMGSQANLVSGCNFSGNFTT
                     TAAGIEGTGNTFQGLSTFTQTSTAGGTRLWGGNTFQGAHVINVTGTAGIQCSQLFAAE
                     TYNSTVTVNLSGTGGVTLARSFDTQFPQNIVLTSTSTGSVAFGFNGGTSTLVNGRTIS
                     IGGLGYTGSFLYLYRFNQVGGTPQTISITGATSQIEMGSAGNATYGCVFDGDWTINAV
                     NYVIAGNTFNGNCTFTKSGAGSDDNSIGANTFNGSFSMTNNCSTNGIFMRFSNIFGAD
                     IYNGPVTLSSNCNIGGMIMGRTFDGFFNHDLIINSTNGYIYLGAGNTLSGVCYLANTR
                     TITTPIFTGGILFFRRFEQLGTTPQTINVTGTSAIEYGGSVASAVTNTFCIFNGDLNS
                     SSGGSTAINNGSFRRNTSISALRFPNVINSIFNENAGNTILSRIAGSNNDDNGGNNIF
                     HGNLTFNNTGSARFRLGINTYGGDTYFGNIEINNSGTGTVSFAYGNTSNPISGNINFI
                     NSNTGTIIFGEASTANSSTLAGTLSALSGNFTNGTISIRFMTQNGGANQFTGTASAVS
                     LVHNNCIVNGTMNISTSGSITITNSTYNNTVTSTVSGTSTIRTNTFQSNANLTSASFI
                     LGGTLAQGNTFNSTSTFLKNGTSNDDTNGGNIFNDQVNFNTSNATGRWRLGTTADDFN
                     GIVNFNQNAAGVLAPSYTAVSTYAGNVNFSSPAGVTITSGSGGGRSTFDGGNAQSINL
                     VSGNNPVFTRLTLNKTSNAVTLNCPITVTVDFLPSSGNLNTTTTNIVTLANNTTTTIG
                     NASSYVNGPMDYAMASNSASRRTLNFPIGKDSDWRPAVLEVSHNSNTSYTYRGELFNS
                     SAEALGWTKPVTVDLVSLVHWWDIDRFLTSTMVSSPNANLRTGAGDRPIITLHYGAND
                     GVTDPLNLVICKNTNGAPTTWDNIGGTGTAAVAGSITSTSNPTVFNSFSRFTLGNLLG
                     GTNPLPF"
BASE COUNT         2312 a         1544 c         1507 g         2054 t
ORIGIN      
        1 ttattgccat gcggatgaga gtacctttca acaggcagat caattgtaca aatcacaaca
       61 atacgattct gccagggttc tttttgaatc gatgctggca gaagacaaac aatcagcgat
      121 tgtctttttt gctttgggta attgctacta ccgcctgagc gaccctgtta ctgcagttta
      181 ctattatgaa aaagcgatta aactggatcc caataatcct gatctgaaag cgaatattgc
      241 ctttgtcaga aaacaattca aagacaaagt gaatacagat cgcagcggca tatcgggatg
      301 ggtgttttca attgttaatg acaatcaccc gaacaaatgg tcggggacgg ctgtaatact
      361 gatcaatttt gcatttctat cattgatact ctcgcgtttt ttgtctgcca agaaatcgcg
      421 gattgccttt atcgccggaa gtgttgcatt ggttcttgga atttccagac taattccggc
      481 atggtaccaa tacagcagta tcaccaatca cgaccaggga attgtaaaaa cagaccgtac
      541 tgaaatcaaa agcgcacctt ctgaaaacag cagcaccgtt tttattctat ctgaaggaac
      601 gaaagtaaac ataacagaaa gcaatgaatt ctggcttgag gtcaatatcg gcgatgccaa
      661 tgttggttgg attcaacgtg aacaaattct tgaaatttga gcagaactaa accatccata
      721 gtaattctgg gaacaggaaa tgttgcctat gccttgggaa ttgccttgtt taaaaatggc
      781 tatcccataa agcaggtata tagccgatcg cacgaaaaag ccttgcagtt ggcccgaaaa
      841 ctgaaagcat ccttcaccga cgatctgcgc gcaatcgaaa tcgatgcttc tgtttattta
      901 ctggcgatca gtgatgatgc cttacccgaa ttttgtaagg cgctcccacg tctaggcgga
      961 attgtaatgc atacatcagg aagcatacca ctctccgttc ttaaaaaaca aaatcacacc
     1021 gctgttattt atcccttaca gacattgcaa aaagatcatc aaccggaatg gcaaaaaaca
     1081 tcctttttgg ttgaagcaaa tgatgaggtc ggattaaaaa tggtaaaaag cattgcgcgt
     1141 acactgacta aaaaagtaat tcaatgcaca tccgaagaac gaaagcaagt ccaccttgct
     1201 gcagttattg caagtaattt cagtaatcac ctgtttgcgc tttcgcaaga tattctccag
     1261 tcaaataaac ttccaactga tcttcttgct ccgctcattg aagagactat caaaaaaagt
     1321 ctgaaaggag atgcaaaaac caaacaaaca ggacctgcac gaagaggtga tcaaaccatc
     1381 cttcgcaagc atctcaacat gctcaataac gacccactta caaaaagtat ttacaaacag
     1441 atttctgctt ctatacagaa aatgtattct aaaaaatcga aaaaatgaat tactggcttt
     1501 taaaatcaga acctgatgtg ttctcctggg atgatctggt aaaaaaagga ccttctgttt
     1561 gggatggagt tcgtaattac caggcacgaa acaatttaaa agctatgaaa aaaggcgata
     1621 aggcactttt ctaccacagc aacattggcc gtgaaattgt aggtattgca accattacca
     1681 aacccttttt tcctgatccg accattgacg acgagcgatg ggtagcagtt gaagtgaagg
     1741 ctcagaaaaa atttaaaaat gctgttacac ttgataccat caaaaatact ccggcacttg
     1801 ccaaactacc actagtaacc cattcgaggt taagtgtgat gccggtttcg gaattggatt
     1861 ttgttttgct ttgcgacatg ggaggaattg tctcataatc aaaatactga aaacagagtt
     1921 caattaattc attgctttag gctttcagct attttattac ttttgctcaa aattgattcc
     1981 tatgggacgc gcgtttgaat accgtaaagc aagaaagatg gcacgctggt cagccatgtc
     2041 gacgactttt accaagattg gtaaagagat cagcatgtcg gtaaaggcag gtggatcaga
     2101 tcccaatacc aatagccgcc tacgcgcagc aatgcaaaac gcacgttctg ctaatatgcc
     2161 taaggcgaat gtagaagcag ccattaaaaa agcaacagat aaggacgctt cgaattatga
     2221 agaagtgaat tatgaaggtt atggtccgaa tggagttgcc attttcattg aaacagcaac
     2281 tgataatacc acgagaaccg ttgccaatat gcgcaccatt ttcaataagg gcggaggatc
     2341 gatcggtact tcaggatcat tggattttgt attcgaacgt aaagccaatt tcacggtaaa
     2401 aaataccggg atcaatgttg aagaactgga gcttgaactg attgatgcag gtttggaaga
     2461 aattgaagcc gatgcagaag aaatctttat tgtaaccagg tttgcagatt tcggagcaat
     2521 gcaaaaagcg ttggaaggaa aaggttttga aattttaagt gcagaaaaag tccgcacccc
     2581 attaagcact gttgaagtca ctcccgaaca acgcgaagcc attgacaaaa tggtagagaa
     2641 gtttgaagaa gatgatgacg tgcagcaagt gtttacaaat atggcataac ccggatcaca
     2701 ggtcacttaa tacttgctat tcaatcttag taaagaaaag tctgtggatt aaccaaaaaa
     2761 cattcatccg aatccataaa atctaatttg attttcaatc atttaggcat cttgtgattt
     2821 atggaatatc aatagcattt ttttttctca agatttcttg tgtttagaat actccaggta
     2881 gtattttagt gtggaaaaca cttacttaca tctatgattc ctttaccgct attccgcatg
     2941 accggaatat tgctattagc attctattca gtaatctcta acgctcaaac cacctatacc
     3001 tggaacggaa taacttcgac ggattggaac acttcaacca actggacgcc taacgggact
     3061 ccatcaccca cagaccatgt tgtgatcgtt acaggatcaa accaatgtac actacccggc
     3121 aatgtggatg ttacaaattt cagtatgaca tccggtactt taaatgtggg agcaaacacg
     3181 ctaactgtaa caggaacttt ttctgctctc aacggaacga tcacgggaac aggaaatctg
     3241 ttcgccaatg gatcatccgt tgtttttgga aacacaatag caggtccaac agtatcacca
     3301 aacgtttcaa tttccacctt aagtgtacga tcgcaacgaa ccacgtatca tggaaacgta
     3361 acgattttaa aaacaattgg tgcaacagca gtagacagct ggcgtggtgg aaacactttt
     3421 aacgggacat tttacctaaa caatgcctca gatgacattg ccgggaacaa tggagacatt
     3481 tattttgggt ccaacaatgg agatcctgtt gatgttttca atggaagaac cacaatgatt
     3541 gtatcgggtg cagcacggat tagaattcct caaaatggtt ctacaatttt caatggggta
     3601 actcattttt tatccaatgg atttggtggg cagcatgata ggatacaacc tgcccgtcaa
     3661 ggcgctgcga atgccgtttt caatgattcg gtatatttta cttgtgcatc agcaacaagc
     3721 gatatgcata ttgcctatga tgcaggcact tcttgtgtat ttaacggacc tgttgtttgt
     3781 aatcgtttaa caggttcagg tggggcattt aatcttgggc ttgacggaac gatcacacta
     3841 aacagtaacc ttattgtaaa taataatagc acaggttcaa taactgttac cagcggttca
     3901 ggcagttcaa ctttggcaaa tggaaattca atttttgtag gagctgaagg atttaattca
     3961 ggatcgcttg atattcgtaa tttcactcaa attggaaata ccactcaaaa tttagtattt
     4021 tcctcagctg ctgcattatt tattggtgct gccggaagtc cgtgtacatt taatgcaaac
     4081 acgattaatt tttcagcggg gcgagttcgc tcacagagca ccacgtataa cagcacttcc
     4141 atttcatttt ccaaaaccgg tgcaactgcc gatgataacg gcgacaattt ttttggtgga
     4201 cctgtcactt tttcaaactc aggaggagca gaattcagat tttctagcac cattgcgagg
     4261 gatgtttttg catcaactgt tactcttgtt aacagcggaa caggtatgat atcgatgtcg
     4321 cgcactttcg ataccgatta tgattttaac attgaaataa acaatccgag tgtaactgcg
     4381 ggaggaggaa ttcaatttgg agcgaacgga ggatcatcaa cccttgcaag tggtagaacc
     4441 atttctgtag gaggcagtgg atatgaattt ggctatttgt atttgttccg ttttaatcaa
     4501 ttaggaggga ctccacaaac cataactctt ggagctaatt ccacacttct ccgaatgggt
     4561 tcacaggcaa atctggtttc gggttgtaat ttttcaggca attttactac aactgcagca
     4621 ggaattgaag gtacaggtaa cacctttcaa ggcttatcaa catttacaca aacctctaca
     4681 gccggaggaa caagattatg gggaggaaac acatttcagg gagcacatgt gatcaatgta
     4741 acgggaactg caggcattca atgctcacaa ttattcgcgg ccgaaacgta taattcaaca
     4801 gtaacagtta atttatcggg aaccggaggc gttacacttg cacgctcatt tgacacgcag
     4861 tttccacaga atatcgtttt aaccagcaca tcaacaggat ctgttgcatt tggttttaac
     4921 ggaggaacga gcactcttgt caacggaaga acgatcagca ttggaggatt ggggtataca
     4981 ggaagtttcc tttatctcta tcgattcaat caggttggag gaacaccaca aaccatttcc
     5041 attaccggag cgacttcaca aatagagatg ggttcggcag ggaatgcaac atatggatgt
     5101 gtttttgatg gagactggac gatcaacgct gtgaattatg tcattgcagg taatacgttt
     5161 aatggcaatt gcacattcac aaaatcagga gccggatctg atgacaacag cattggagcg
     5221 aacacattca atggaagttt ttcaatgacc aacaattgtt cgaccaacgg tatcttcatg
     5281 cgtttttcca atatttttgg agctgacatc tataatgggc ctgtaacttt atcttccaat
     5341 tgcaatatcg gaggaatgat tatgggccga acttttgatg gtttttttaa tcatgatctg
     5401 atcattaatt ccaccaatgg ttacatttat ctgggagctg gaaacacatt atcaggagtc
     5461 tgttatctgg ccaacacacg aaccattacc actccaattt tcaccggagg tattctcttt
     5521 ttccgtcgat ttgaacaatt gggtacaaca cctcaaacaa ttaatgttac aggaacttca
     5581 gcaatagaat atggtggaag tgttgcaagt gccgtaacca atactttttg catttttaat
     5641 ggtgatttaa attcctcttc cggaggaagc acagccatta acaatggttc attcaggaga
     5701 aataccagta tatctgcact ccgttttcca aatgtaataa attccatttt caatgaaaat
     5761 gcaggaaaca ctatcctttc gcggattgcc ggttcgaata acgatgataa tggcggaaat
     5821 aatattttcc atggtaatct cacgtttaac aacaccggtt ccgcacgttt tcggttagga
     5881 atcaacacct atgggggcga cacctacttt ggaaacattg aaatcaataa cagcggaact
     5941 ggaacagttt catttgccta cggaaacacc agcaatccga tttccggaaa tatcaatttc
     6001 attaacagca atacaggtac catcattttt ggagaagcct cgactgccaa ttcaagcaca
     6061 cttgcaggca ctctttcagc attatcaggt aatttcacca atggcaccat ttcgattcgt
     6121 tttatgacac agaatggtgg tgcaaatcaa tttaccggaa ctgcatctgc tgtttcactg
     6181 gtacacaaca attgcattgt gaatggaaca atgaatatct ctacatccgg ttccattacg
     6241 attaccaatt caacttacaa caatacggtt acatcaacag tgtccggcac aagcactatt
     6301 cgtacgaata cgtttcagtc taatgccaat ttaacttccg cttcgtttat acttggagga
     6361 acacttgcac aaggaaacac attcaattca acctccacct ttttgaaaaa cggaacatcc
     6421 aatgatgata ccaatggtgg aaacatattc aatgatcaag tcaatttcaa cacttccaac
     6481 gctacaggaa gatggcgtct tggaacaact gctgatgatt ttaatggtat tgtcaatttt
     6541 aatcaaaatg cagcaggagt actcgctcct tcctatactg cagtcagcac ctacgccgga
     6601 aatgttaatt tttcttcgcc ggcaggagta acaattactt caggatccgg tggaggacga
     6661 agtacttttg acggaggaaa tgctcagtcc atcaatttag tttcaggaaa caacccagtg
     6721 tttacccgat taaccctcaa taaaacctcc aacgcagtaa cgcttaattg cccaataaca
     6781 gtaactgtag attttctacc cagttccggt aatttaaata caacaacaac caatattgtt
     6841 acccttgcaa acaatacaac caccactatc ggaaatgcct cgagttatgt gaatggtcca
     6901 atggattatg caatggccag taacagtgct tccaggagaa cactgaattt tcccattggt
     6961 aaagactccg actggaggcc tgctgtactt gaagtttccc acaattcaaa cacttcctac
     7021 acctacagag gagaattatt taacagcagc gcagaagccc tgggatggac aaaaccggtt
     7081 acagtagatt tggtatcatt ggttcactgg tgggatatag atcgctttct taccagcact
     7141 atggtatcgt cacccaatgc gaaccttaga accggagctg gtgaccgtcc gattatcacc
     7201 ttgcattacg gtgcgaacga tggtgtaacg gatccgttaa atctggtaat ttgtaaaaac
     7261 acaaatgggg caccaacaac ctgggacaac atcggcggaa caggtacagc agcagttgca
     7321 ggaagtatta cttcgacatc caatccaacg gtatttaatt ccttcagcag atttacactt
     7381 ggtaatttat tgggaggaac caatccgtta ccgtttg
//
LOCUS       DAOMYG010000111         7397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3358_length_7397_cov_10.779760, whole genome shotgun sequence.
ACCESSION   DAOMYG010000111 DAOMYG010000000
VERSION     DAOMYG010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3358_length_7397_cov_10.779760"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PK610_04925"
     CDS             complement(<1..379)
                     /locus_tag="PK610_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRJ35306.1"
                     /translation="MAILDFQRPDKVIMISSDDRVGQFEFRPLEPGYGITIGNSLRRI
                     LLSSLEGYAITSVKIEGADHEFSSLKGVAEDVTEIILNLKQVRFKRQINAENEHVHIS
                     IKGQEKLTAGDLGRFTSSFQVLNP"
     gene            complement(390..998)
                     /gene="rpsD"
                     /locus_tag="PK610_04930"
     CDS             complement(390..998)
                     /gene="rpsD"
                     /locus_tag="PK610_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRJ35307.1"
                     /translation="MARYKGPKSKISRRFKENLFGRIDKALEKKNYPPGQHGPNKKRM
                     KQSEYAVQLNEKQKAKFIYGILERQFENLFKEASRMKGITGHNLLALCEARLDNTVYR
                     MGIGNTRRAARQLVAHRHVTVNGTVVNVPSYQLNPGDVVEVRERSKALEPIVNSVAAS
                     SKRFDWIDFNAGDMKGTFLNRPDREQIPENIKEQLIVELYSK"
     gene            complement(1028..1423)
                     /gene="rpsK"
                     /locus_tag="PK610_04935"
     CDS             complement(1028..1423)
                     /gene="rpsK"
                     /locus_tag="PK610_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009087288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRJ35308.1"
                     /translation="MAKQTDDKKKKKKKVAVEAVGQAHISATFSNIIISLTNATGQVI
                     SWSSAGKMGFRGSKKNTPYAAQVAAEECSKEAFENGLRRVKVFVKGPGSGRESAIRTI
                     HNSGIEVMEIVDVTPLPHNGCRPPKKRRV"
     gene            complement(1449..1826)
                     /gene="rpsM"
                     /locus_tag="PK610_04940"
     CDS             complement(1449..1826)
                     /gene="rpsM"
                     /locus_tag="PK610_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRJ35309.1"
                     /translation="MARIAGIDLPKNKRGEIGLTYIFGIGRSTARKILDNAGVSYDKK
                     VSEWNDDETGKIRTFIGDNFKVEGALRSEVSLNIKRLMDIGCYRGVRHRVGLPLRGQT
                     TKNNARTRKGKRKTVANKKKATK"
     gene            complement(1845..1961)
                     /gene="ykgO"
                     /locus_tag="PK610_04945"
     CDS             complement(1845..1961)
                     /gene="ykgO"
                     /locus_tag="PK610_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HRJ35310.1"
                     /translation="MKVKASIKKRSAECKIVRRKGRLYVINKKNPKFKQRQG"
     gene            complement(1974..2192)
                     /gene="infA"
                     /locus_tag="PK610_04950"
     CDS             complement(1974..2192)
                     /gene="infA"
                     /locus_tag="PK610_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRJ35311.1"
                     /translation="MAKQGVIEQDGTIVEALSNAMFRVELENGHIIIAHISGKMRMHY
                     IKILPGDKVRVEISPYDLTKGRISFRYK"
     gene            complement(2194..3498)
                     /gene="secY"
                     /locus_tag="PK610_04955"
     CDS             complement(2194..3498)
                     /gene="secY"
                     /locus_tag="PK610_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRJ35312.1"
                     /translation="MKDLVKTLKNIWKIEELRQRIVLTFTLILIYRVGSYVVLPGVNS
                     AGLNQGGPSGLAGLLDLFAGGAFSRASIFALGIMPYISASIIMQLLGIAVPSIQKMQR
                     EGESGRKKLNQWTRVLTIFITAFQAPGYIATSVPAAAQTESFSYPLTIMILIAGTMFI
                     MWLGERITDKGIGNGISLLIMIGIIAKLPGAFAQELVSRHTSGGYVVLLVEVVLLLLV
                     IAFSILLVQGTRRIPVQFAKRIVGNKQYGGVRNYIPLKVNSAGVMPIIFAQAIMFIPM
                     YINQATEGRWFSGLVNFEGFWYNFIFAIMIILFTYFYTAIMVNPRQMAEEMKRNGGFI
                     PGVKPGKQTESFIDSLISRITLPGAIFLALVAILPAFAMMIGIKQQFAYFYGGTSLLI
                     MVGVVLDTLQQIETHLLSRHYDGLMKSGKIKGRGQQMPSMVS"
     gene            complement(3521..3967)
                     /gene="rplO"
                     /locus_tag="PK610_04960"
     CDS             complement(3521..3967)
                     /gene="rplO"
                     /locus_tag="PK610_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010181911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRJ35313.1"
                     /translation="MKLNNLKPAKGSVKENKRLGRGQGSGRGGTSTRGHKGDKARSGH
                     KTKIGFEGGQMPIQRRLPKFGFKNINRVEYKGLNLDAIQALVEKRNLSEIDPEVLFAN
                     GLVGKNDLVKILGRGELKAKVKISAHAFSATAKSAIEGKGGETVVL"
     gene            complement(3983..4165)
                     /gene="rpmD"
                     /locus_tag="PK610_04965"
     CDS             complement(3983..4165)
                     /gene="rpmD"
                     /locus_tag="PK610_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009391662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRJ35314.1"
                     /translation="MGKIIITQIKSSIDRPKDQKATLRALGLTRIRQSVEKERNPQID
                     GMVRKIMHLVEVSEVK"
     gene            complement(4177..4695)
                     /gene="rpsE"
                     /locus_tag="PK610_04970"
     CDS             complement(4177..4695)
                     /gene="rpsE"
                     /locus_tag="PK610_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRJ35315.1"
                     /translation="MANENVKRVKATDIELKDRLVGVNRVTKVTKGGRTFSFSAIVVV
                     GNENGIVGHGLGKAKEVTEAISKGIDDAKKNLIKVPIVNGTVPHFQEGRFGGARVLLK
                     PAASGTGVIAGGAMRSVLESVGVKDVLAKCQGSTNPHNVVKATIEALVQLRDAYTVAQ
                     QRGISMDKVFNG"
     gene            complement(4718..5068)
                     /gene="rplR"
                     /locus_tag="PK610_04975"
     CDS             complement(4718..5068)
                     /gene="rplR"
                     /locus_tag="PK610_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRJ35316.1"
                     /translation="MATKESRRARIKRSIRTKLSGTTQRPRLSVFRSNKQIYAQIIDD
                     IKGVTLVSAGSLKNDDAQKVNKVDQARLIGKEIAEKAIKAGIQEVVFDRNGYLYHGRV
                     KTLAESARENGLKF"
     gene            complement(5079..5636)
                     /gene="rplF"
                     /locus_tag="PK610_04980"
     CDS             complement(5079..5636)
                     /gene="rplF"
                     /locus_tag="PK610_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRJ35317.1"
                     /translation="MSRIGKAPIEIPKGVEVKIDGNSVAVKGPKGELTQMVNPSVAIS
                     VEDNQIILKRTGEEKQTRALHGLYRALLNNMVVGVSKGYKIEQELIGVGYRANANGQQ
                     LELSLGFSHNVVFELPKEIKVITTAEKGQAPRIVMESHDKQLIGMVAAKIRSLRKPEP
                     YKGKGIRFLGENVRRKAGKSAGAKK"
     gene            complement(5655..6053)
                     /gene="rpsH"
                     /locus_tag="PK610_04985"
     CDS             complement(5655..6053)
                     /gene="rpsH"
                     /locus_tag="PK610_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRJ35318.1"
                     /translation="MVTDPIADYLTRVRNAIKANHKVVEIPASNLKKSMTKILFEKGY
                     ILNFKFEDDNKQGTIKIALKYHPVTKTSAIRKLERISRPGLRKYTGSDSLPRVLNGLG
                     IAILSTSKGLMTDKEARKENVGGEVLCFVY"
     gene            complement(6079..6348)
                     /gene="rpsN"
                     /locus_tag="PK610_04990"
     CDS             complement(6079..6348)
                     /gene="rpsN"
                     /locus_tag="PK610_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HRJ35319.1"
                     /translation="MAKESMKARDRKRAKMVAHFADKREALKKAGDWDALQKLPRNSS
                     STRVRNRCQLTGRPRGYIRQFGVCRNVFREMALNGLIPGVTKSSW"
     gene            complement(6358..6909)
                     /gene="rplE"
                     /locus_tag="PK610_04995"
     CDS             complement(6358..6909)
                     /gene="rplE"
                     /locus_tag="PK610_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019039500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRJ35320.1"
                     /translation="MSYVPRLKSKYKEEIIPALQKQFNYKSSMQVPRITKICINQGLG
                     DAVADKKLVESAVKELSTIAGQKAVPTVSRKDISNFKLRKGMPIGARVTLRGDNMYEF
                     LDRLISVSLPRTRDFRGVNAKGFDGRGNYTFGVKEHIIFPEIDIDKVNKILGMDITFV
                     TTATTNEEAFSLLKEFGFPFTKK"
     gene            complement(6909..7223)
                     /gene="rplX"
                     /locus_tag="PK610_05000"
     CDS             complement(6909..7223)
                     /gene="rplX"
                     /locus_tag="PK610_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRJ35321.1"
                     /translation="MQKKLHIKKGDTVLVIAGESKGKQGKVVTIDREKSRVIVEGVNL
                     VKRHTKPDAKNPQGGIVEKEAGIHISNLMLVADGTPTRVGRKVENGKIQRISKKSKGV
                     IK"
     gene            complement(7248..>7397)
                     /locus_tag="PK610_05005"
     CDS             complement(7248..>7397)
                     /locus_tag="PK610_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL14 family ribosomal protein"
                     /protein_id="HRJ35322.1"
                     /translation="GSYIRFDDNAVVLLNAQGEMRGTRIFGPVARELREKQYMKIVSL
                     APEVL"
BASE COUNT         1810 a         1756 c         1578 g         2253 t
ORIGIN      
        1 caggattcaa cacctggaaa gaactggtaa agcgaccaag gtcacctgca gtaagttttt
       61 cctggccttt gatgctgata tgaacgtgtt cgttctctgc attgatctga cgcttaaaac
      121 gaacctgctt gaggttaagg ataatttcag ttacgtcttc tgcaacacct ttcagtgaag
      181 agaattcgtg atccgcacct tcaattttca ctgaagtgat ggcgtatcct tcgagtgaag
      241 aaaggagaat acgacggagc gagtttccga tagtgatacc gtaaccgggt tcaagaggac
      301 gaaactcaaa ctgacctaca cggtcgtctg agcttatcat gatgactttg tccggtcttt
      361 gaaaatcaag gatggccata gatcgattat tatttagaat agagttcgac gatgagttgt
      421 tctttaatgt tttcggggat ctgttcgcga tcaggacgat tgaggaaagt acccttcata
      481 tcacctgcgt tgaaatcgat ccagtcgaaa cgctttgagc ttgctgcaac ggagttaacg
      541 attggctcca gcgcttttga acgctcgcgt acttcaacta catcaccggg attgagctgg
      601 taacttggta cgttaaccac tgttccgttt actgtaacgt gacggtgagc aacaagctga
      661 cgagctgcac ggcgggtatt accgatgccc atgcggtaaa cagtattgtc taaacgagct
      721 tcgcaaagtg ctagcaagtt atgacctgta atacctttca tgcgcgaagc ttccttgaag
      781 agattctcaa actgacgctc aagaattccg taaatgaatt ttgctttctg cttttcgtta
      841 agctgaacag cgtattcaga ttgcttcatg cgcttcttgt tcggaccatg ctgtccggga
      901 ggataattct tcttttcgag cgctttatcg atgcggccaa agagattctc cttgaagcga
      961 cgtgaaattt ttgatttggg gcctttgtat cgtgccatgt tgaattatta tttctaaaaa
     1021 aggaatatta aactcttctc ttcttgggag gacgacaacc gttgtgcggt agcggagtaa
     1081 cgtcaacaat ctccatcact tcaatacctg aattgtgaat ggtacggatt gctgattcac
     1141 gtcctgatcc gggtcctttt acgaacacct tcacacgacg gagtccgttc tcgaacgctt
     1201 ccttggagca ctcttcagca gcaacctgcg ctgcatacgg agtattcttt ttggaaccgc
     1261 ggaatcccat ctttccggca gaagaccagg agatcacctg tccggttgcg ttggtaaggg
     1321 agatgatgat gttgctgaat gttgcgctga tatgcgcttg tccaacagct tccactgcta
     1381 cctttttctt ctttttcttt ttgtcgtcgg tctgctttgc cataacctca attatatctg
     1441 ctcatttatt acttagtggc ctttttctta ttggcaacgg tcttacgctt ccctttacgg
     1501 gtacgcgcat tgttcttggt ggtttgtcca cgcagcggta agcctacgcg gtgacgaaca
     1561 ccacggtaac aaccgatgtc cataagacgc ttaatattca gagaaacctc agaacggagc
     1621 gcgccttcta ctttaaaatt atctccgatg aacgtacgga tctttccggt ttcatcatcg
     1681 ttccattcgc ttactttctt atcgtaagac actcccgcgt tatcgagaat ttttcgtgcg
     1741 gtgctacggc ctattccgaa aatataggtg aggccgattt ctcctctttt gttctttggt
     1801 aaatctatac cagcaatcct tgccatgttc gattattaaa atggttaacc ttgacgttgc
     1861 ttaaattttg ggttcttttt gttgatcaca taaagacggc cctttctccg aacgatctta
     1921 cattcggctg agcgcttttt aatggaggct ttgactttca tcttgctctg ttgttatttg
     1981 tatctgaatg atattcttcc ttttgttaaa tcgtagggtg agatctcaac acgcacctta
     2041 tctccgggga gaattttgat gtagtgcatg cgcatcttcc ctgaaatatg ggcgatgatg
     2101 atgtgcccgt tttccagctc aacacggaac atagcgttcg acaatgcttc aacgatggtt
     2161 ccatcctgct ctatgactcc ttgtttcgcc atcttaactt accatgctgg gcatttgttg
     2221 accacgtccc ttgatcttac cgctcttcat caatccgtca taatgacgac tgagtaagtg
     2281 cgtttcgatc tgctgaagtg tatcgagtac cacaccaacc ataatcagca atgaagttcc
     2341 gccgtagaaa taggcaaact gttgcttgat tccgatcatc attgcaaatg cagggaggat
     2401 cgccaccagt gcaaggaaaa ttgcaccggg gagggtaatg cgggaaatta aactgtcaat
     2461 gaacgattcg gtttgtttac ccggttttac acccggaata aatccgccgt ttctcttcat
     2521 ttcttctgcc atctgacgag gattcaccat gatagcagta tagaaataag tgaagaggat
     2581 aatcatgatt gcaaaaatga agttgtacca gaaaccttcg aaatttacaa gtccggagaa
     2641 ccaacgacct tcagttgcct gattgatgta cattgggatg aacatgatcg cctgagcaaa
     2701 aatgataggc attacacctg cagaattaac tttaagcgga atgtaattac gaactcctcc
     2761 gtattgtttg tttccaacaa ttcgcttcgc aaactgcacc gggattctgc gtgtaccctg
     2821 tacaagaaga atagagaacg caatcaccaa aagcagaagc acaacctcaa cgagtaacac
     2881 aacgtatccg ccgcttgtat gacgcgaaac caattcctga gcaaatgcac cgggtagttt
     2941 ggcaataata ccaatcatga taagcaatga aattccgtta ccgatcccct tatcagtaat
     3001 tctttcgcca agccacatga taaacatggt acctgcaata agaatcataa tagttagagg
     3061 atagctgaac gattctgttt gtgcagcagc cggaacagat gttgcgatat aacccggagc
     3121 ctggaatgct gtaatgaaaa tggtgagtac acgggtccac tgattcagtt tctttcttcc
     3181 gctttcacct tcgcgttgca ttttctgaat ggaaggcact gcaataccca acaactgcat
     3241 aatgatagat gcagagatgt atggcataat tccaagtgca aagatcgatg cacgagaaaa
     3301 cgctcctccg gcaaaaagat caagcaatcc tgccagacct gatggtccac cttggttcaa
     3361 tccggcagaa tttacccccg gaagcaccac gtaggaacca acacggtaga tcaggatcag
     3421 agtaaaggtc agcacaatgc gctgacgaag ctcttcgatc ttccagatgt tctttaatgt
     3481 ttttactagg tccttcatct atgttgtgat gttagttcga tcagagaacc actgtttctc
     3541 cacctttgcc ttcaatagct gactttgcag tagctgaaaa tgcatgtgcg gatattttaa
     3601 cttttgcttt cagttcacca cgacctaaaa ttttcaccag gtcatttttg ccaactaatc
     3661 cgtttgcaaa aagaacttcc ggatcaattt cgctcaggtt tcttttttct accaatgcct
     3721 gaattgcatc aaggtttaaa cccttgtact caacacggtt aatattcttg aaaccaaact
     3781 tgggaagacg acgctgaatt ggcatctgtc caccttcaaa tccgattttg gttttgtggc
     3841 cggaacgcgc cttgtcacct ttgtgaccac gtgtagatgt tccaccacgg ccggaacctt
     3901 gtccgcgacc aagtcgctta ttttccttta ctgaaccttt tgcaggtttg agattattca
     3961 gtttcatcgc tcaggtttgt ctttatttca cttcgcttac ttcgacaagg tgcatgatct
     4021 tacgtaccat gccatcgatt tgtgggtttc tttccttctc caccgactgg cgaatacggg
     4081 tgagtccaag cgcacgtaat gtggcctttt gatctttcgg acgatcgatc gaactcttga
     4141 tttgcgtaat gatgatctta cccatgtcgg ttataattat ccgttaaaca ctttatccat
     4201 tgaaattccg cgttgctgag ctacagtata agcatcacgt aattgtacga gcgcttcaat
     4261 tgttgctttt acaacgttgt gcgggttggt tgaaccctga cactttgcaa gtacgtcttt
     4321 cactccaacg ctctcgagca ctgaacgcat cgcaccaccg gcaataacac cggtaccact
     4381 tgctgcaggc ttaagaagca cacgagctcc accaaaacgg ccttcctgaa aatgagggac
     4441 cgttccgtta acgataggaa ccttaataag attcttcttt gcatcgtcaa tccccttaga
     4501 gatggcttca gtaacttcct tggctttacc aagaccgtgg cctactatgc cgttctcatt
     4561 acctaccact acgatggcag aaaaactaaa tgtacgtcca cccttggtaa ccttggtaac
     4621 acggtttaca cctaccaagc gatccttgag ctcgatatcc gtagccttaa ctctttttac
     4681 gttctcgttg gccatattgc gtattgttct ttcttaatta gaatttcaaa ccattttcac
     4741 gtgccgactc ggcaagtgtc ttaacacggc cgtggtaaag gtaaccgtta cggtcgaata
     4801 ccacttcctg aattccggct ttgatcgctt tttctgcaat ctctttaccg ataagacgtg
     4861 cctgatcaac tttgttaact ttctgtgcat cgtcgttctt gagtgaacct gccgacacaa
     4921 gagtaactcc cttgatatca tcaatgatct gcgcgtagat ctgtttgttg ctgcggaaga
     4981 ccgaaaggcg gggacgttgt gtagtaccgc taagtttggt ccggatgctg cgcttaatcc
     5041 tggctctgcg tgattcttta gttgccattt ttcacttatt attttttcgc accggctgat
     5101 tttccggcct tacgtctaac attttctcca aggaaacgga taccttttcc tttgtacggt
     5161 tcaggtttac gcagtgaacg tattttcgct gctaccattc cgatcagttg cttatcgtgc
     5221 gactccatca caatacgtgg ggcctgtcct ttttctgcag tggtaatcac cttgatttcc
     5281 tttggcaatt caaacacaac gttgtgtgag aaaccaagtg aaagttcaag ctgctgacca
     5341 tttgcattgg cacggtaacc tacaccgatc aattcctgct cgatcttgta acctttcgat
     5401 actcctacaa ccatattgtt aagaagagca cggtacaatc cgtgaagagc acgggtctgc
     5461 ttctcctcgc ctgtacgctt gaggatgatt tggttgtcct caacgctgat agcaacagaa
     5521 gggttaacca tttgggtaag ttcgcccttt ggacctttaa cagcaactga gttaccgtcg
     5581 atcttcactt ctactccctt agggatttcg atgggggctt ttcctatacg tgacatgatt
     5641 cgattctctt tggattaata aacaaaacac aacacttctc cacctacgtt ctctttacgt
     5701 gcttctttgt ctgtcatcag acctttggaa gtagagagaa tagcgattcc caatccgtta
     5761 agaacacggg gtaaggaatc ggatccggtg tatttacgaa gacctggacg gctgatacgc
     5821 tctaatttgc ggatggcgga ggtcttggtt actggatgat acttaagtgc gattttaatc
     5881 gtaccctgtt tgttgtcatc ctcgaactta aagttcagaa tgtaaccctt ttcgaaaagg
     5941 atcttggtca ttgacttctt gaggttcgac gctggaatct ccaccacctt atggttggct
     6001 ttgatagcgt ttcttactcg ggtcaaatag tctgcaatgg gatcggttac catttttaat
     6061 gctttatagc ggttattatt accaacttga tttagttact cccgggataa ggccgttgag
     6121 agccatttca cggaatacgt tacgacacac tccgaactga cggatatacc cgcgaggacg
     6181 tccggtgagc tggcaacgat tgcgtactcg tgttgaagaa gagttacgtg gaagcttttg
     6241 taaagcatcc caatcgcctg ctttcttcag ggcctcgcgc ttatccgcga agtgggctac
     6301 cattttcgca cgttttctgt cacgtgcttt cattgattct ttcgccatgg ctctgtctta
     6361 ttttttggtg aacgggaatc cgaattcttt gagcaatgag aacgcttcct cattggttgt
     6421 tgctgtggtt acaaaagtga tatccatacc gaggatcttg ttcaccttgt cgatgtcaat
     6481 ctccgggaat atgatatgtt ctttcacacc gaaagtatag ttaccgcgtc cgtcgaaacc
     6541 tttcgcattc actccacgaa agtcgcgggt acgtggcagc gaaacagaga tcaaacgatc
     6601 gaggaattcg tacatattat cgccacgaag cgttacacgt gcaccaatcg gcataccctt
     6661 acgaagtttg aagttcgaga tatcttttct tgatacagtt ggaaccgctt tctgacctgc
     6721 aatagtagaa agttctttta cggcagattc aaccagcttc ttgtcagcta ctgcgtcgcc
     6781 caacccctga ttgatacaga tcttggtgat gcgtggcacc tgcattgaac tcttgtagtt
     6841 gaattgcttc tgaagcgcag gaatgatttc ttccttgtac ttcgatttaa gtctgggaac
     6901 gtagctcatt acttaatcac cccctttgat ttttttgaaa tgcgttgaat ttttccgttt
     6961 tcaaccttac ggcctacacg ggttggcgtt ccgtctgcta ccagcatcag gtttgagatg
     7021 tggataccag cttccttctc cacgattcca ccttgtggat tcttagcgtc gggcttagtg
     7081 tggcgcttaa cgaggttaac accttccaca attacgcggc tcttttcacg atcgatagtc
     7141 acaactttac cttgcttgcc tttcgattct ccggcaatca ctaatactgt gtcgcctttt
     7201 ttgatatgta gtttcttctg cataatccta tttctgttca ggttgcatta aagcacttca
     7261 ggtgcaagtg aaacaatttt catgtactgc ttctcacgta gttcacgtgc taccggtccg
     7321 aaaatacgtg ttccacgcat ctcgccctgt gcattcagca ataccactgc attgtcgtcg
     7381 aaacggatgt aagaacc
//
LOCUS       DAOMYG010000112         7391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3367_length_7391_cov_8.550981, whole genome shotgun sequence.
ACCESSION   DAOMYG010000112 DAOMYG010000000
VERSION     DAOMYG010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7391
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3367_length_7391_cov_8.550981"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2293
                     /locus_tag="PK610_05010"
     CDS             <1..2293
                     /locus_tag="PK610_05010"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRJ35323.1"
                     /translation="NNNYVRDFSEDQFGNLWFATYGGGISKFNGQSFFHFNKQNGVFD
                     NDVRCLMFDNKNELWFGTFGGGIGKYDGKNILQLTEEQGLCNNYILCMEKTHDGKLWF
                     GTNGGGISIYDGTAFTTFDEETGLSNNTVLCITEDKNKSVWIGTNGGGVIRYDGRTFT
                     HFDEKTGLSNNTVRSILADRNGTIWFGTSGGGICNYDGEKFIHITEKEGLKSNYILSI
                     LEDKEGNIWFGTRFGLSKLSTAQQSELNNKIANGSTKESDVYFKNYAYEDGFLGIGVN
                     GGKTIYQDKKGLIWIGSNDRLTQLHPDGEIKNNKSPEIRITNLNLFNEKIPWQVLELK
                     QDSVLKLGNGVSVGDFEFSGTMNWNTLPENLSLAFNNNYLTFQYIGITMHQPDLVKYR
                     YILEGWDENWSSISTRTEVAYGNLQEGEYTFKVKAMNSEGLWSEPASYTFSIRPPWWK
                     TTWFKSSYISAFILLIIGSFRWRTASLRRRQKQLEQTVIERTEELVLKNQQVEKQKEI
                     VEEKNKEILSSITYAKRIQEAILPGFQRWKRYLPESFILYTPKDIVAGDFYWMEKSQN
                     FLFIAAADCTGHGIPGAMVSVICSNALTRAISEEKLSDTNKILNRVREIVIENLSGKE
                     EETVRDGMDICLIRIDTNKPGEIQFSGANRPLWIARNKQLLEFKPDKQAISFSESTKD
                     FNAALIQLEKGDTLYLFTDGYADQFGGNYGKKFMYKRLKELLIAISNDSPAQQKEKLH
                     TNFNTWKGELDQVDDVCFIGIRL"
     gene            complement(2364..5171)
                     /locus_tag="PK610_05015"
     CDS             complement(2364..5171)
                     /locus_tag="PK610_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRJ35324.1"
                     /translation="MNIKHLIIGLFYLFSGGDLFSQSRNNPEADSLLGVWRNEAQSDT
                     VRLFAVHTLIWEHLLFSQPDSAYRLSQQQELFSEKSGSKNYVADAINSQGVSLAIRGE
                     NDRALLKFEQVLKIKTELNDSSGIAGAIVNMGNIHRDIGNYALATSCYQNSRGIYAGM
                     KNWQGWANAIDNLGIVLSYSGNLTGAIDYFEKAKAIRLEKNDQRGLPTSYSNLATAYN
                     DLGVSDKAVQWYILAMELHTKNGNKQGIATAMNNLAIQYKQKGKYAAAMDMYRESLLL
                     RKKINDKNGISSTLNNIGNLYRVMNEKDSAMFYFHRSLEVRKSLNDKLAIAFAMNNIG
                     SYHHDLGNYDSALVYLNNALEIAQSIRSTSEIRRALRYQFATYYRRAAIPTAAEKLRG
                     LMEIRQKDIEINLALLAETDKEKYFATMEEDFLLHDAFMVNHNSTRPDLTIQSYNQAL
                     LLKGLILRGSGNLRADILASSDSLLIESYYSWLTVKRKIAKAYSRGKDATALEEEAQS
                     LEATLIRKSSAFKQHLEEKNVDWKQVQHSLVKGEAAIEFLRIPLLDLHRSENNLPDEV
                     MYVALVIKKDFLQPLYIPLCKEEHLQALIAQKSSDRKTKIESAYTFSGRESLYSLLCQ
                     PLESVLNDVRTIYFSPAGLLHKISFSAIRNRDGRYVSSVIELNPLLSTGSLIQRKKSS
                     ASGIGPLFLFGGVDYTDTLNANVGWTFLEGTLTEVESIQRKALQQKKSVKKFTGKNAG
                     EVQFKSCSGEKGILHIATHGFFYPDPSAAKDTTHVEIDAEIVFRSGARKEKDLFFLSS
                     PHSMMRSGLVFAGANKLWSEHSVSESEEDGILTAQEITGLDLSGFLLIVLSACETGLG
                     DVRGYEGVYGLQRAFKVAGAHYLIMSLWEVPDKETAEFMELFYGVLLKNGNIRKSFQI
                     AQNAMRKKYSPYYWAAFVLVE"
     gene            complement(5346..6350)
                     /locus_tag="PK610_05020"
     CDS             complement(5346..6350)
                     /locus_tag="PK610_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ35325.1"
                     /translation="MATPDRAQWDVQLFVDPNQIAVSLAGVCWTGTEFWLSKWSDDSL
                     FTANASGVKTSGFVIPGISATRSITSDGTFLYLANNSNTIYKVNPSTKTLVSSFTSSA
                     SGTTRYISYDPTLNSGAGGFWTGNFATDWVAISTSGAVLSTIAQSTHGLSGVYGLVYD
                     PYTPGGPYLWASDQSGPSTVMLIQHSILGVPTGLSYDPTGDIPVTPGVAGGAFACNNF
                     VSGTASLGLLSQNVALLVYEMGSNVSVNDADPIACFSVFPNPTSDAFSIEFESKSGGE
                     IQISVSDILGKTVHSANYYPLPGKNRIQINSLPLENGMYMVSLIQGENVVSTPLVVSK
                     "
     gene            6844..>7391
                     /locus_tag="PK610_05025"
     CDS             6844..>7391
                     /locus_tag="PK610_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35326.1"
                     /translation="MAKQTAKKTTKATTKKKVDKPTAKKAVKKTAPKTEKAKPTKKVI
                     KKAPAKPTVKAKKVVVADTPTVIKKTFDKITLISHKFTEPNEHPTKLKPNKLSVSEIN
                     GIGGNTYLKFHSAGIKTVEDFVNAGVSKISQVLGWTEKGGLPFYLRGLAVYQNRVIKI
                     KAPKNISQSPIYLDIETDLSQRT"
BASE COUNT         2364 a         1533 c         1569 g         1925 t
ORIGIN      
        1 gaacaataat tatgttcgtg atttttcaga agatcaattt gggaatttat ggttcgcaac
       61 ctatggtgga ggaataagca aattcaacgg acaatccttt ttccatttca ataaacaaaa
      121 tggagttttt gataatgatg tcagatgcct gatgtttgac aacaagaacg agctttggtt
      181 cgggactttt ggtggaggaa tcggtaaata cgatggcaaa aacattcttc agctaacgga
      241 ggaacaaggt ctgtgtaata attacatttt gtgcatggaa aaaacccatg atggcaaatt
      301 gtggttcgga acaaatggtg gtggaatttc gatttatgac ggaacagcat ttacaacttt
      361 cgatgaagaa actggcttaa gtaacaatac agtgttatgc attacagagg acaaaaacaa
      421 atcggtctgg ataggaacca atggcggagg agtcatcagg tatgacggac gaacattcac
      481 tcacttcgat gaaaaaaccg gattaagcaa caatacggta agaagtatac tcgccgaccg
      541 aaatggaact atctggtttg gaacaagtgg aggaggaata tgcaactacg atggtgaaaa
      601 atttatccat ataaccgaaa aggaggggtt aaaaagcaac tatatcctta gcatactgga
      661 agacaaagaa ggcaatattt ggtttggtac acgctttggg ttgagcaaac tttctacagc
      721 acagcagagt gaattgaaca acaagattgc caacggcagt acgaaagagt ctgatgtcta
      781 ttttaagaat tatgcctatg aagacggttt tctgggtatt ggcgtaaacg gaggaaaaac
      841 aatttaccag gataaaaaag gattgatttg gattggaagt aatgacaggt tgacacaact
      901 gcaccctgat ggagaaataa aaaacaacaa gtctcccgaa atcagaatta ccaatctgaa
      961 tttattcaac gaaaaaattc cctggcaagt actggaacta aaacaggata gtgttttaaa
     1021 gcttggcaat ggcgtaagcg ttggagattt cgaattttcg ggaaccatga actggaacac
     1081 cttacccgag aatctgagtc tcgccttcaa caacaactac cttactttcc aatacattgg
     1141 catcaccatg catcaaccgg acctcgtaaa atacagatac attttagaag gatgggatga
     1201 aaactggagt tcgatcagca cacgaacaga agttgcctat ggtaatttgc aggaaggaga
     1261 atatacgttc aaggtgaaag ccatgaacag cgaaggatta tggagtgaac ctgcatccta
     1321 caccttttcc attcggccac cctggtggaa aaccacatgg ttcaaatcaa gttatatctc
     1381 tgcattcatc ttactgatta ttggctcatt cagatggaga acagcttcat tgcgacgaag
     1441 acaaaaacaa ttggaacaaa ccgtaattga acgaactgaa gaacttgttc taaaaaatca
     1501 acaggtcgaa aaacaaaaag aaattgttga agaaaagaac aaggaaattt tgtcgagtat
     1561 aacttacgcc aagaggatac aggaagccat tttacccgga tttcagcggt ggaaaaggta
     1621 tttaccggaa agttttattc tctatacacc aaaagatatt gttgcaggtg acttctactg
     1681 gatggaaaaa agtcagaatt tcctgttcat tgcagcagca gattgtaccg gacacggcat
     1741 acccggagcc atggtgagtg taatctgttc gaatgcttta acaagagcca tctcggaaga
     1801 aaaactatca gacaccaaca aaattctgaa tcgtgtacgt gaaattgtga tcgaaaacct
     1861 atcgggcaaa gaagaagaaa ccgttcgcga cggaatggat atttgcctga tccgaataga
     1921 caccaacaaa cccggtgaaa tacaattcag cggagccaat cgtccgctgt ggatagcacg
     1981 gaataaacaa ctgctcgaat tcaagccgga taaacaagcc ataagttttt ctgaaagcac
     2041 gaaagatttt aacgctgctc ttattcaact tgaaaaagga gatacccttt atcttttcac
     2101 agatggatat gccgatcagt ttggcggcaa ctatggcaag aaatttatgt acaaacgtct
     2161 gaaagaatta ttgattgcca tttctaatga ttcgcctgca cagcagaaag agaaattaca
     2221 taccaacttc aacacctgga aaggagaact tgatcaggta gatgatgtat gttttattgg
     2281 aatccgtttg tagcaattct ccatcagttt tctttttctt caatatggaa aatacggaat
     2341 tatagttcct aaaacaaagt cccctactcc accaaaacaa aagcagccca gtaatacgga
     2401 ctgtattttt ttcgcatcgc gttttgtgcg atttgaaagg acttacggat attgccgttc
     2461 ttgagcaata caccataaaa caattccata aactcagccg tttctttatc aggaacttcc
     2521 cacaacgaca tgatcaggta atgtgctcct gctactttaa aggcacgctg caaaccatat
     2581 acaccttcat agccacgaac atctccgagt ccggtttcgc aggcggataa tacgatcaat
     2641 aaaaatccgg ataaatccaa tccggtaatt tcctgagcag tgagaattcc atcttcttcc
     2701 gattcggaaa ccgagtgctc cgaccatagt ttgttggcac cggcgaatac aagcccggat
     2761 cgcatcatgg aatgcggtga ggacaaaaag aacagatcct tttccttacg cgctccgcta
     2821 cgaaagacga tttctgcatc aatttccaca tgcgtggtgt cttttgcagc agagggatcg
     2881 ggatagaaaa aaccatgtgt tgcaatatgt aatattccct tctcacccga acaggatttg
     2941 aattgcactt caccggcatt ttttcctgtg aatttcttta ccgacttctt ttgctgcagc
     3001 gccttccttt gaatggattc cacttccgtt agtgtgccct ccaaaaatgt ccagcccaca
     3061 tttgcgttca gcgtatccgt ataatccact cctccgaata aaaacagcgg accaattccc
     3121 gatgcagaag atttttttcg ttgaatcaaa ctacctgttg acagtaaggg atttaattcg
     3181 attaccgaag aaacatacct accatcgcga ttccgaatag cagaaaatga aattttgtgc
     3241 agcaaacccg caggagaaaa ataaatggtc cgtacatcat tcaacacgga ttccaagggc
     3301 tgacacaata gcgaatacaa actttccctg ccggaaaaag tataagcaga ttcaattttt
     3361 gttttgcggt cggatgattt ttgtgcgatc aatgcctgca aatgctcttc cttacacaag
     3421 ggaatataaa gcggttgcaa aaaatctttt ttgattacca gcgccacata catcacttca
     3481 tccgggagat tgttttctga acgatgcaga tcaagaagag gaatgcgtaa aaattcgatg
     3541 gccgcttcgc cttttaccag tgaatgctga acctgtttcc aatcgacatt cttttcttcg
     3601 aggtgttgct taaacgcact cgattttcgg atcagtgtag cctcaagcga ttgcgcttct
     3661 tcttccagtg ctgttgcatc ttttcctctg gaatacgcct tggcaatttt tcgtttaacc
     3721 gttaaccagg agtaatacga ttcaatcagc aaggaatcgc tgcttgccaa aatatctgca
     3781 cgaagattcc cgctgccgcg caatattaat cccttcagca acaaagcctg gttgtaggac
     3841 tgaatggtga gatcaggacg cgtggaattg tgattcacca taaaagcatc atgaagcaga
     3901 aaatcctcct ccatggtggc gaagtacttt tccttatccg tttcggcaag taatgcgaga
     3961 ttgatttcaa tatccttttg gcggatttcc atcagtcctc tgagtttttc cgcagcagtt
     4021 ggaatggcgg cgcgacggta atacgtagca aactgatacc tcaatgcgcg acggatctcg
     4081 gaggtggatc ggatcgactg tgcaatttcc agtgcattat tcaaatagac caatgcgcta
     4141 tcgtaattac caagatcgtg atggtaggaa ccgatattgt tcattgcaaa agcaatagcc
     4201 aatttatcgt taagtgattt acgcacttcc agactccggt gaaaatagaa catcgcagaa
     4261 tccttttcat tcatcaccct gtacagattc ccaatgttgt tgagtgtaga agaaattccg
     4321 ttcttatcgt ttattttttt gcgcaggagc agactttccc tgtacatatc cattgcagca
     4381 gcgtacttgc ccttttgttt gtactgaata gccagattgt tcatagcagt tgcaatgccc
     4441 tgtttgttgc cgtttttggt atggagttcc attgccagaa tgtaccattg aaccgcttta
     4501 tcggagactc caagatcatt gtaagccgtt gcaagattgg aataagaagt gggcaatccg
     4561 cgctgatcat ttttttcgag acgaatggcc ttggcttttt caaaataatc gattgcaccg
     4621 gtaagatttc cggaatagga taaaacgatt cccaggttat caatggcatt tgcccatccc
     4681 tgccagtttt tcattcccgc ataaatacct ctgctgttct ggtagcagga agtcgccagt
     4741 gcataattgc cgatatcgcg gtggatattc cccatattaa cgatagcacc ggctattccc
     4801 gaggaatcgt tgagttcggt ttttattttt agtacctgct cgaatttgag cagtgcgcga
     4861 tcattttcac cgcggatggc caacgaaaca ccctgagaat tgatcgcatc ggccacatag
     4921 ttcttcgaac ccgatttttc cgaaaacaat tcctgctgct gtgaaagacg gtaggcacta
     4981 tcgggttgcg aaaacaagag atgctcccaa ataagcgtat gcaccgcaaa caagcgtacg
     5041 gtgtcggact gcgcttcatt tctccataca cccaaaagcg aatcggcttc cggattattg
     5101 cgggattggg aaaacaagtc gccgcccgaa aacaaataga ataagccaat gatgaggtgc
     5161 ttgatattca ttggcagtaa actaataagg aaaaagtgtt ttcgcaattt tattggacga
     5221 atggattcaa ttgttctctc aaagcaattt cctgaagcat ggagaatcgg tttgaaaaaa
     5281 gcaatcaaat cacatcgact gaagaagttt ccaaatcaac cttacagttt acaatttgct
     5341 ttctatcatt tgctcactac caacggcgta gaaactacat tctctccctg aataagagaa
     5401 accatgtaca tcccattttc gagaggaagt gaattgatct gaatgcggtt ctttccgggt
     5461 aaaggatagt agttcgcgga atgaacagtc ttacccaaaa tatccgaaac cgaaatctga
     5521 atttctcctc cggatttgct ttcaaattca attgaaaacg catcggaagt tggattggga
     5581 aaaacgctga agcacgctat cgggtctgca tcatttacag acacattcga acccatctcg
     5641 tagaccaata aagctacgtt ctgtgaaagt aacccgaggc ttgcagttcc ggaaacaaaa
     5701 ttattacagg caaatgcgcc acctgccact ccgggagtga cgggaatatc acctgtagga
     5761 tcgtaggata atccggtagg cacacccaaa atagaatgct gaatcagcat cacagtactg
     5821 ggtccactct gatcggaagc ccacaaatac ggaccaccgg gggtgtacgg atcgtaaacc
     5881 aaaccatata cacctgacaa accgtgtgta ctctgcgcga tggtagatag tacagcaccg
     5941 cttgtgctga tagcaaccca atctgtggcg aagttaccgg tccagaaacc acctgctcca
     6001 ctatttaggg tgggatcata actaatgtac cgtgttgttc cggatgccga cgaagtgaac
     6061 gagctcacca atgttttagt ggaaggattt accttgtaaa tggtattcga attgttggcc
     6121 agatagagga aagtaccatc cgaagtaatg gaacgggttg ccgatattcc ggggatcaca
     6181 aatccggatg tttttacacc tgaagcattc gctgtaaaga gtgaatcatc gctccacttc
     6241 gacaaccaga attcggtgcc tgtccaacac actcctgcca gactgacggc aatttgattg
     6301 ggatcaacaa aaagctgaac atcccattgt gcacggtcgg gtgttgccac ttgttgagca
     6361 gtgatgggag cagcatcgag agctgatttt gtgcaaaagc gccaagagga agagcagttc
     6421 caatgacaaa gaatagattc agtaaattga gtttcatagc agctgttttt ctcaaaattc
     6481 ctcatcctga aggcagtgaa aaatgacaag aatcaggata aaaaaccgat tgggtaagtt
     6541 ttcatttgct aataaagcat aaatggtaaa gtcggttcag gttaatttaa ttattgagca
     6601 gaagagggtc ccggttaaat agggttaaag gggaattggc acacaattgc tgcaagacgc
     6661 ttgaaagcaa acgcaatcaa agaagaaaaa aacctgaaaa ggaagaatta tatagatgat
     6721 ttatagaatt tttgataaat tggataatcc aattaaaagt aagcatagca cgccggatta
     6781 gcgagccatg cttaataaag gttgggttat aaatgagtta taagcgattt taaaaagacg
     6841 acaatggcga aacagacagc aaagaaaacg actaaagcga ccacgaaaaa aaaggttgat
     6901 aaaccgactg caaaaaaagc ggttaagaaa actgcaccta agactgaaaa agccaaaccg
     6961 acaaaaaagg ttatcaagaa agctcctgca aaaccgaccg ttaaggctaa aaaagtagta
     7021 gttgccgaca caccgacagt tattaaaaag acttttgata aaatcacttt aatctcacat
     7081 aaatttacag aacctaacga acacccgaca aagcttaaac ctaacaaatt aagcgtatca
     7141 gaaatcaacg gcattggagg aaacacctac ttgaaatttc attcggcagg aattaaaaca
     7201 gtcgaagact ttgtaaacgc tggtgtttcc aaaatttcac aagtattggg ttggacagaa
     7261 aaaggtggtc taccttttta tttaagaggg cttgcagttt accagaacag agtaatcaaa
     7321 ataaaagctc cgaaaaatat ttctcaaagc ccaatttact tagacatcga aaccgacctt
     7381 tctcaaagaa c
//
LOCUS       DAOMYG010000113         7368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3379_length_7368_cov_10.942705, whole genome shotgun sequence.
ACCESSION   DAOMYG010000113 DAOMYG010000000
VERSION     DAOMYG010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7368
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3379_length_7368_cov_10.942705"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PK610_05030"
     CDS             complement(<1..965)
                     /locus_tag="PK610_05030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ35327.1"
                     /translation="MNQNKHILVLAEWFPHEEDPQLGVFIQKHCEIISRFARVTVVFV
                     NGNDKQKEKIRIREEFKGYRLIRASYRKSWFRPLHLHRYTMAMKKIRKMVGDVDLIHI
                     QVVGRNYLASKFIFKGIPFVITEHWSGYLREGTEPLIGTTYTREAFQNAQAVSTVSAP
                     LATAIEGKFDRKDIQIIPNLIEAADSSTSMKSAALRMIIVADLVDEIKNISGIILALK
                     ELEPEENIHVEIIGEGKDRKDLESLSKTISNKHISIHFAGRKTNHEVLKELAVADFMV
                     MNSRTETFSMICAESIMAGTPVIATRCGGPESFVHSGNGILIGVD"
     gene            complement(1024..2304)
                     /locus_tag="PK610_05035"
     CDS             complement(1024..2304)
                     /locus_tag="PK610_05035"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRJ35328.1"
                     /translation="MVLNYIWIGFFLISFVVVIVRLFLEGDLSIVSFMMEEALRMAEF
                     SVMNIALPLAGIMIFWMGMMKIAENSGLVNVLAKMMWPFFKYIFPDVPKGHPAAGSMM
                     MNFSANFLGLDNAATPLGIKAMKELQSMNPTPETASNSQIMFLVLNTSGLTLIPTSII
                     ALRTANHSANPTDIFIPILLATTIATLVALTTVSIYQRINLLRAGFVIPVIGLLTIVA
                     SIVGLILYFATCNQSGLTPEQLSSCASEKMGTYSGFAGAFIILLILTSFVAYGAWKKI
                     NVYDSFIEGARDGFATVVQIIPYLVAMLVGIAVFRASGSMNLITDAIHFLVSLITVKT
                     EFVDALPTAMMRPLSGGGSRALMIETWNTYGIDSLRGHMTSVIQGSTETTFYTIAVYY
                     GAVGIRNVRHTIQCGLIADGSGIIAAIVISYLFF"
     gene            complement(2295..2873)
                     /locus_tag="PK610_05040"
     CDS             complement(2295..2873)
                     /locus_tag="PK610_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRJ35329.1"
                     /translation="MLTELNILVVDCGSSKVPDIVNALSQNGIRVGQKKLSELNGAEN
                     EYQGIVISGAPILLTEEDHKKYSSRILPLLNKERPLLGICFGHQLLGLLHNTGVVRCD
                     EDRGWQEITIIHPGGIFEGFNNWVMLKEDHCECIGLPDGFRRTAGSSVCANEAMQHEK
                     YPWYGVQFHPEVSGEQGQNIFNNFIKICRTWF"
     gene            complement(2873..3490)
                     /locus_tag="PK610_05045"
     CDS             complement(2873..3490)
                     /locus_tag="PK610_05045"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRJ35330.1"
                     /translation="MLLEIHPANPDERKIRKVVDCLNAGGVVVFPTDTVYAIGASLMK
                     PKAIEQMAKIKGVRAEKANFSLICYDLSSLSEFATVDTPVFKVMKKALPGPYTFILNA
                     NNKISRYFGYTKRTVGIRVPDNNIIRTIVRDLGHPIITTSVHDDDEILDYTTDPSVIH
                     ERYEKQVAIVIDGGYGHNEASTVIDCTEGYPQVIREGIGPTENLI"
     gene            complement(3626..4285)
                     /gene="fsa"
                     /locus_tag="PK610_05050"
     CDS             complement(3626..4285)
                     /gene="fsa"
                     /locus_tag="PK610_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HRJ35331.1"
                     /translation="MKFFIDTANLDQIREANDLGVLDGVTTNPSLMAKEGISGTERVR
                     KHYVDICSIVDGDVSAEVIATDYEGIIKEGEELAKLHKNIVVKVPMIRDGIKAIKYFT
                     QKGIRTNCTLVFSAGQALLAAKAGATYVSPFIGRLDDVSTDGLALIEDIRTIYDNYGY
                     DTEILAASVRHPMHIIDCAKIGADVATCPLSAIVALLKHPLTDSGLAQFLADHAKAAA
                     K"
     gene            complement(4407..4506)
                     /gene="ffs"
                     /locus_tag="PK610_05055"
     ncRNA           complement(4407..4506)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK610_05055"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4686..6566
                     /locus_tag="PK610_05060"
     CDS             4686..6566
                     /locus_tag="PK610_05060"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006261247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRJ35332.1"
                     /translation="MAQQSNYTEDNIRSLDWKDHIRLRPGMYIGKLGDGSAADDGIYI
                     LLKEILDNCIDEFVMGFGNKVDIRITEREVFIRDYGRGIPLGKMIDAVSKMNTGAKYD
                     TKAFKKSVGLNGVGTKAVNALSDSFMVASHREGQVKKAWFKQGELTKESKLEKSSEEN
                     GTAVTFTPDPTIFKNFHYRDGFISKMLWNYCYLNPGLTIYFNGQAYFSENGLLDLLKE
                     NMTEEPLYDIIHLRGDDIEVAMTHTNSYGEEYYSFVNGQHTTQGGTHQGAFREAVVKT
                     IREFFRKEYEAVDVRQSICAAVAVKVIEPVFESQTKTKLGSNEIEPGGKSMRAFIADF
                     LKEHLDNYLHKHPEVADALQRKILAAERERKELSGIRKLARERAKKSSLHNKKLRDCR
                     VHLTDMKDPRRLETTLFICEGDSASGSITKVRDVATQAVFSLKGKPLNTHGLTKKIVY
                     ENEEFNLIQAALDIEDDMENLRYNNIVVSTDADVDGMHIRLLLLTFFLQFFPDLVKNG
                     HVYILQTPLFRVRNKKETMYCYSEEEKQVAMKKLGRNPEITRFKGLGEISPDEFRMFI
                     GQNMRIEPVVVGKESIQHLLDYYMGKNSQDRQDFIIGNLRVEKDVVEAADEVEQTEET
                     EA"
     gene            6563..>7368
                     /locus_tag="PK610_05065"
     CDS             6563..>7368
                     /locus_tag="PK610_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008272128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRJ35333.1"
                     /translation="MIHEDENLDKDGGLFTGSDGDNDDQGRTTVLGGMYKDWFLDYAS
                     YVILERAVPSVVDGLKPVQRRILHSMRELEDGRYNKVANLIGHTMKYHPHGDASIGDA
                     LVQIGQKDLLIDTQGNWGNILTGDSAAAARYIEARLTKFALDVVFNPKTTQWQASYDG
                     RNKEPINLPAKFPLLLAQGGEGIAVGLSCKVLPHNFLELIDACVDYLKGKKFEIYPDF
                     PTGGSADFSNYNDGMRGGRVRCRAKIRMASGKQLIIDEIPFGTTTSSLIDS"
BASE COUNT         2129 a         1529 c         1648 g         2062 t
ORIGIN      
        1 ttgtcaaccc caattaaaat gccattaccg gaatgcacaa aagattccgg tcccccgcat
       61 cgggtagcaa ttacaggagt gcctgccatt atggattctg cacagatcat cgaaaaggtc
      121 tcggttcggc tattcattac cataaaatct gccaccgcaa gttccttgag gacttcatga
      181 ttcgtttttc ttcctgcaaa gtggatgctg atatgcttat tcgaaatggt tttgcttagt
      241 gattcaagat cttttcggtc ttttccctcg ccaatgatct caacatggat gttttcttcc
      301 ggttcaagtt ctttcaatgc gagaatgatt cctgaaatgt tttttatttc atccactaaa
      361 tcggcaacaa ttatcattcg cagtgcagca gatttcatgc ttgtgcttga atcagcagct
      421 tcaataagat tgggtatgat ctgaatatct tttctgtcaa atttcccttc aatagccgta
      481 gccaaaggag ccgatacggt gcttaccgct tgtgcatttt gaaaggcttc acgggtataa
      541 gtcgttccaa ttaaaggttc cgtgccctcc cgtaaatagc ccgaccaatg ttccgtaatt
      601 acgaatggaa tgcctttaaa aataaatttc gatgctaaat aattcctacc cacaacctga
      661 atgtggatca gatcgacatc ccccaccatt tttctgattt tcttcatagc catggtatag
      721 cgatgaaggt gcaaaggacg aaaccaactt ttccgatagc ttgctctgat cagccggtag
      781 cctttaaatt cctccctgat ccgaatcttt tctttttgct tgtcatttcc atttacaaaa
      841 actacggtaa cgcgggcaaa acgcgaaatg atttcacaat gtttttgaat gaatacaccc
      901 aattgggggt cttcctcatg aggaaaccat tcggcaagaa caagtatgtg tttgttttga
      961 ttcaaattga ttgcgctatg aaattgaatc tattgattag ggaggccaaa aattgtatca
     1021 attttagaaa aacaaataac tgatcactat tgctgcaata atacccgatc cgtcggcaat
     1081 taagccgcac tgaatggtgt gtcttacgtt gcgaatgccc actgctccgt aatacacggc
     1141 gatggtgtag aaggtagtct cagtgcttcc ctgaattacc gatgtcatgt gtccgcgtaa
     1201 actatcgatt ccgtaggtgt tccaggtttc gatcattaat gctctcgaac caccgccgct
     1261 gagtggtcgc atcatggctg ttggaagtgc atcaacaaat tctgttttta cagtgatcaa
     1321 tgaaactaga aaatgaatgg catcagtaat gagattcatg gaacctgagg ccctgaatac
     1381 agcaatgcct accaacatgg caaccagata aggaataatt tgaacaacgg ttgcaaaacc
     1441 atctctggcg ccttcaatga agctgtcgta aacattaatt tttttccagg caccataggc
     1501 aacaaatgat gtaagaatca aaaggatgat aaatgcgcct gcaaaaccgg aataggtgcc
     1561 cattttttcg gatgcgcagg aactgagttg ttcgggtgtg agtcctgact ggttgcaggt
     1621 ggcgaaatac aatatcaacc ctacaatact cgctacaatg gtgagtaaac caattacggg
     1681 aatcacaaat cctgctcgca ataaattgat gcgttgataa atggaaactg tagttaaggc
     1741 aacaagcgtg gcaatagtgg ttgcaagcag tatcggaata aaaatatcgg ttgggttggc
     1801 agaatgattt gctgttctca aggcgataat ggatgtggga atgagtgtaa gtccggaagt
     1861 attcagcaca agaaacatga tttgagaatt gcttgctgtt tccggtgtcg gattcatgct
     1921 ttgcaattct ttcatcgctt taataccaag tggtgttgca gcattgtcga gaccaagaaa
     1981 attggctgag aaattcatca tcatcgagcc ggctgcaggg tgacctttgg gtacgtccgg
     2041 aaaaatgtat ttgaaaaaag gccacatcat ttttgccaat acattcacta agcccgaatt
     2101 ctctgcaatc ttcatcatac ccatccaaaa gatcatgatg cctgcaagcg gtaatgcaat
     2161 attcatgaca gaaaattcag ccatccgcaa tgcttcttcc atcataaaac ttacaatgga
     2221 aagatctcct tcgagaaaaa ggcggacaat caccactaca aatgagatca gaaaaaatcc
     2281 tatccagata taatttagaa ccacgttctg cagattttaa tgaaattatt aaagatgttt
     2341 tgtccttgct caccggaaac ttccggatga aactgaacgc cataccaagg gtacttttcg
     2401 tgttgcatcg cttcattggc gcaaacagaa gatcctgctg ttcttcggaa tccgtcgggt
     2461 aacccaatgc attcacagtg atcttccttc agcatcaccc aattgttaaa tccctcaaaa
     2521 attcctccgg gatgaataat cgtaatctcc tgccagccgc ggtcttcatc gcagcgtacg
     2581 acgccggtgt tgtgaagcag acccaataac tgatgtccaa aacaaatacc cagcagcgga
     2641 cgctctttgt tcagcagtgg taaaatccgt gatgaatatt ttttgtgatc ttcctctgta
     2701 agcagtattg gtgctccgct tatgacaatt ccctggtatt cattttcagc tccgttcagc
     2761 tcagaaagtt ttttttgtcc gacacgaatt ccgttttgcg aaagtgcatt tacaatatcc
     2821 ggaactttac tgcttccgca atccaccacc agtatattga gttccgttag catcagatca
     2881 ggttttcggt tggaccaatt ccttcgcgga ttacctgcgg atatccttct gtacaatcaa
     2941 ttaccgttga tgcttcatta tgaccatatc caccgtcaat cacaatggca acctgctttt
     3001 cgtaacgctc atgaataacc gaaggatcag tagtataatc cagtatttca tcatcatcat
     3061 gtactgaagt tgtaatgata ggatgaccta aatcgcgtac aatagtgcgt atgatgttgt
     3121 tatcggggac acgaattcct acagtacgtt tggtatagcc gaaatacctg gagattttgt
     3181 tgttcgcatt gagaataaac gtgtacggac ccggaagcgc ttttttcatg actttgaata
     3241 ccggagtatc aacagttgca aattctgaaa gcgaactcag atcgtaacag atcagcgaga
     3301 agtttgcttt ttctgcacgg acacctttga tttttgccat ttgttcaatg gcttttggtt
     3361 tcatcaacga agcgccaatg gcatagactg tatcagtcgg aaaaacgaca actccacctg
     3421 cattcaggca atccaccact ttgcggatct tgcgttcgtc tggatttgca ggatggattt
     3481 cgagtaacat gactgaaaga taagaactcg ggtgactgaa acaaacggca atggaaaata
     3541 gaattcaaga ttgtgctgtt aatccgcgat ggcaaaaaaa aagctgcatc atttacgatg
     3601 cagctctttc aatagcattt gaatattatt tagctgctgc tttggcatga tcagcaagaa
     3661 actgtgctaa tccgctgtca gttagggggt gcttaagtaa ggcaacaatt gcagagagcg
     3721 gacaagttgc cacatcagct ccaatttttg cgcaatcgat aatgtgcata ggatgacgaa
     3781 ccgatgcagc cagaatttcg gtgtcgtatc cgtaattgtc gtaaatggtg cggatatctt
     3841 caattaatgc aagcccatca gtgctaacat cgtcgagtcg accaatgaat ggcgaaacat
     3901 aagttgcacc tgcttttgct gcaagtagag cttgtcctgc agaaaatacg agagtgcaat
     3961 tggtgcggat tcctttttga gtaaaatatt ttatggcttt aattccatcg cggatcattg
     4021 gaacttttac cactatgttt ttatgaagtt ttgcgagttc ttcgccttcc ttgatgattc
     4081 cttcgtagtc ggtagcaatt acttctgcgc ttacatcgcc atctacaata gagcagatat
     4141 cgacataatg tttccgaaca cgttccgttc cggagattcc ttctttggcc atcagagagg
     4201 gattggtagt tacgccatcc agtacaccaa gatcattggc ttctctgatt tggtcgagat
     4261 ttgcagtgtc gataaaaaat ttcattgtgc gttttttgtc aaaaatagcc tgcaggacga
     4321 caatgcccaa atgaaatagt taacagatgt tgataagaag ggtagtgggt aggttagcga
     4381 ctgatttcgc tgagaaagaa aaaaaaatag ggcaagctga ttgcatcgca ccgctacaac
     4441 cacccgtcct tgctccgttc ccggcctggg gaattggggt gggagctggt cgtgcaagac
     4501 ttgccccgcg caaagataga aaaagaccga cattcagatg ctttgcagtg aaaaaacagg
     4561 gaaaagcttg attttcttag tctttccgag gttgataact atgaaacaat gttgaaaaat
     4621 caaaatttat ccacagttct aaggggtttg cattgtgtaa ttttggaacc cttgtaaaac
     4681 cgactatggc acaacagagt aattatacgg aagacaatat ccggtccctg gactggaaag
     4741 accatatccg ccttcgtccg ggtatgtata tcggtaagct gggcgacggt tcggctgctg
     4801 atgatggaat ttatattctg ctcaaagaga ttctcgataa ctgtatcgat gaatttgtaa
     4861 tgggcttcgg aaataaagtc gatatccgca tcaccgagcg cgaagtgttt atccgcgatt
     4921 atggtcgtgg tatcccgctt ggtaaaatga tcgatgccgt ttcgaaaatg aatacgggag
     4981 cgaaatacga taccaaggca tttaaaaaat cagtgggatt gaatggtgtt ggtacaaaag
     5041 ctgtaaatgc actatctgat tcctttatgg tggcttctca ccgtgaaggt caggtgaaaa
     5101 aggcctggtt caaacaagga gagcttacca aagaatcgaa acttgaaaag tcttctgaag
     5161 aaaatggaac agcagtaacc tttactccgg atccaaccat tttcaagaat ttccattacc
     5221 gcgacggatt catctcgaag atgttgtgga attactgtta tctcaatccg ggactaacta
     5281 tttattttaa cggtcaggct tatttttctg agaatggtct tttggatctg ctcaaggaaa
     5341 acatgaccga agagccgctt tacgatatca ttcatttgag aggggatgat attgaggttg
     5401 caatgacaca caccaattcc tacggtgaag aatattacag ttttgtaaat ggtcagcata
     5461 ccacacaggg agggacacat cagggagcat ttcgtgaagc tgttgtaaaa acgatacgtg
     5521 aatttttcag aaaagaatac gaagccgttg atgtgcgtca gtcgatatgc gcagcagttg
     5581 ctgtaaaagt aattgaacct gtatttgaat cgcaaaccaa aaccaagctt ggctccaatg
     5641 agattgagcc cggtggtaaa tcgatgcgtg ctttcattgc tgattttctc aaggaacacc
     5701 tcgataatta cctgcacaaa cacccggaag tggccgacgc gcttcagcgt aaaatacttg
     5761 cagccgagcg agagcgtaaa gagttaagcg gaatacggaa actcgcccgt gagcgcgcga
     5821 agaaatcgtc gctgcacaat aaaaaattac gcgattgccg tgtacacctt acggatatga
     5881 aagatccgcg tcgtttggaa accacactgt tcatttgtga gggtgattct gcaagtggtt
     5941 ccatcacaaa agttcgcgat gttgccacac aggcagtatt ttctctaaag ggtaagccac
     6001 tgaatacaca tggacttacc aagaaaatcg tttacgaaaa cgaagagttc aaccttattc
     6061 aggctgcgct ggatatcgaa gacgatatgg agaatctgcg ctacaacaac atcgttgtgt
     6121 caacagatgc cgatgtcgac ggaatgcaca ttcgtctgct gttattgact ttcttccttc
     6181 agttttttcc agatctggta aagaatggtc acgtttatat tttgcaaacc ccgcttttcc
     6241 gtgtccggaa taaaaaggaa acgatgtatt gttattccga agaagaaaaa caagtggcaa
     6301 tgaaaaaact ggggcgcaat ccggagatta cccgattcaa gggattggga gaaatttcac
     6361 ctgatgaatt cagaatgttt atcggtcaga atatgcgcat tgagccagta gttgtaggaa
     6421 aagaatcgat acagcattta ctggattatt acatgggtaa aaactcacag gatcgtcagg
     6481 attttatcat tggcaatttg agggttgaaa aagatgttgt ggaagcagca gatgaagttg
     6541 aacaaactga agaaacagaa gcatgattca cgaagacgaa aatctcgata aggatggcgg
     6601 tttgtttacc ggatcggatg gcgacaatga tgatcaggga agaacgactg tattaggagg
     6661 aatgtataag gactggttcc tggattacgc atcgtatgta attcttgagc gtgcagttcc
     6721 ctctgtggta gatggcctga aaccggtaca gcgcagaatt ctccattcga tgcgcgagct
     6781 tgaagacgga cgctataaca aagttgcgaa tctgattggt cacacgatga agtaccaccc
     6841 tcacggtgat gcttccattg gtgatgcact tgttcagatt ggtcagaaag atttgttgat
     6901 cgatacacaa ggaaactggg gaaatatttt aacaggcgac agtgccgctg ctgctcgtta
     6961 tattgaagct cgccttacaa agtttgctct tgatgtggtg ttcaatccaa aaactacaca
     7021 atggcaggct tcgtatgatg gccggaataa ggaacccatc aatctacctg caaagtttcc
     7081 gcttttgctt gcgcagggag gggaagggat cgccgtagga ttatcctgta aggtactgcc
     7141 acataatttt ctggagctga tcgatgcctg tgtggattat ctgaaaggta aaaagtttga
     7201 gatctatcct gatttcccaa ccggtggttc tgctgatttc tccaactaca acgacggaat
     7261 gcgtggtggc agggtgcgtt gccgtgcaaa aatcagaatg gcttcaggaa agcaattgat
     7321 cattgatgaa atcccattcg gtacaaccac ttcttcgctt atcgattc
//
LOCUS       DAOMYG010000114         7350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3386_length_7350_cov_8.227553, whole genome shotgun sequence.
ACCESSION   DAOMYG010000114 DAOMYG010000000
VERSION     DAOMYG010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3386_length_7350_cov_8.227553"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..2107)
                     /gene="dnaK"
                     /locus_tag="PK610_05070"
     CDS             complement(209..2107)
                     /gene="dnaK"
                     /locus_tag="PK610_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRJ35334.1"
                     /translation="MSKIIGIDLGTTNSCVSVMEGNQPVVIPNSEGKNTTPSVVGFID
                     GGERKIGDPAKRQAITNPRNTVYSIKRFMGNTFDEVSKEVSRVPYEVVRGENNTPRVK
                     INDRMYTPQEVSAMILQKMKKTAEDYLGHEVTEAVITVPAYFNDAQRQATKEAGEIAG
                     LKVRRIINEPTAASLAYGLDKKNQEMKIVVFDCGGGTHDVSVLELGDGVFEVKATDGD
                     THLGGDDFDQKIIDWLAEEFQKEEGLDLRKDPMALQRLKEAAEKAKIELSSTSSSEIN
                     LPYIMPVNGIPKHLVRTLSRAKFEQLVDDLVKRTIEPCRSALKNAGLSVNDIDEVILV
                     GGSTRIPAVQAAVKSFFGKEPSKGVNPDEVVAVGAAIQGAVLTGEVKDVLLLDVTPLS
                     LGIETMGGVMTRLIDANTTIPSKKAETFSTASDNQPSVTIHVLQGERPMARDNRTIGQ
                     FNLDGIPPAQRGVPQIEVTFDIDANGILNVSAKDKATGKEQKIRIEASSGLSKEEIDR
                     MRKEAEANAETDRQAKESVEKLNAADSLIFQTEKQLNEFGDKLPADKKAPIEAALADL
                     KKAYETKDMPSIETAMNQLNQVFQSAAQEMYANTGGAPGADAGNQGAGAGDSEVTDVD
                     FEEVKDKK"
     gene            complement(2268..3317)
                     /locus_tag="PK610_05075"
     CDS             complement(2268..3317)
                     /locus_tag="PK610_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35335.1"
                     /translation="MKKLFVALSVSLALLSSCSTDFDIIGPYKDNTIVFGLLDKNESI
                     HFIKVNKSFLGTGNAYDYAAIKDSSEYALVSGNVEAWNSGTLVASYPLQDTTITNRDS
                     GAFYYPEQTMYYFIATLNQNYEYRLNLNINDGAKEVTGSTKLVYDFPLNTLTGNTLYP
                     FSLAYNNSSLVNNYPDYYIRFTPGINTRRIDTWVHFWYDEYTASGTTRKKIEWKVDSY
                     ITSSTAGTEPERVAVFAGDAFYRYVKLKIPSNANVIKRVARQMDIQFVAVSDDMYTYM
                     KVNEPSTGIVQERPSFTNLSNAIGIFSSRYTKWVRGKVLNKDSQKELCTGQFTYDLLF
                     CTDSLPWSTETYYCP"
     gene            3446..4264
                     /gene="panB"
                     /locus_tag="PK610_05080"
     CDS             3446..4264
                     /gene="panB"
                     /locus_tag="PK610_05080"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRJ35336.1"
                     /translation="MSASAKEIKRVTTHVLHEMKNRGEKISMLTAYDYSMARIVDDSG
                     IDIILVGDSASNVMAGHETTLPITLDQMIYHASSVVRAIHRCLVVVDLPFGSYQGNSK
                     EALNSAIRIMKESGAHAVKLEGGREIKESVERILTAGIPVMGHLGLTPQSIYKFGTYV
                     VRAKEEEEADRLIQDAHLLAESGCFAIVLEKIPAKLASRVAKEVKVPIIGIGAGQGVD
                     GQVLVVHDMLGINNEFQPRFLRKYADLNQIIGDAVKQYIVDVKAKDFPNEKESY"
     gene            4267..4956
                     /locus_tag="PK610_05085"
     CDS             4267..4956
                     /locus_tag="PK610_05085"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRJ35337.1"
                     /translation="MELQVIFEDNHLIAVNKQSGDIVQGDKTGDVPLSEVIKEYLKAK
                     YNKPGNVFCGVIHRIDRPVTGVVLFAKTSKALSRMSEQFRDKQVKKTYWAIVKNKPAS
                     EKGNLVHYLLKDERTNKSKAFSKEVKDAKFSELNFKLIASGERYHLLEVDPLTGRHHQ
                     IRAQLSAMGCPIKGDLKYGSERSNPDGSISLHARRIEFVHPVTGEQQIIEATVPDDRL
                     WKDLEKMASAG"
     gene            5013..5531
                     /locus_tag="PK610_05090"
     CDS             5013..5531
                     /locus_tag="PK610_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35338.1"
                     /translation="MNYFRISNRANTVLLSWEISSGNTCNGTSILRGVDSLQMNEVAA
                     IDGLCGDPGFAVSYSFADSFPPLGQKVFYRIDFGGLGLSKLLETVVYDLTPGYSVLYE
                     NDGFRVVFANVSGSITPFTLFDLTGRTILKSSTNQGSFFVPYSQLSTGVVVFAVELPE
                     GKSIRGKIAISR"
     gene            5682..>7350
                     /locus_tag="PK610_05095"
     CDS             5682..>7350
                     /locus_tag="PK610_05095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HRJ35339.1"
                     /translation="METIEISPKSALQKFFGFSKFKGHQEAIIKNLLAGNHSFVIMPT
                     GGGKSLCYQLPALMMEGTAVVVSPLIALMKNQVDSIRTFGSDDSVAHFLNSSLSKAEI
                     LKVKKNIMEGRTKLLYVAPESLTKQENIDFLTTIKVSFFAIDEAHCISEWGHDFRPEY
                     RKLRPMIDRVGENVPIIALTATATPKVQLDIQKNLGMQDATLFKASFNRDNLFYEVRP
                     KRDVVKEIVRFIKTKQGKSGIVYCLSRKKVEELAEVLRVNGIKALPYHAGLDASTRAK
                     HQDMFLMEDADVIVATIAFGMGIDKPDVRYVIHHDIPKSLESYYQETGRGGRDGGEGH
                     CLAFYSYKDVEKLEKFLNGKHVSEIEIGKQLLHEIVSFAETSMCRRKYLLHYFGEGFK
                     EEDCGKMCDNCRNPREKTEGKDEVALLLEAILETKEKHKPSHIVSILLGELTSDVRTY
                     KHNKLEVFGKGSESTDRFWNAVVRQTLVAGLIRKDIETYGVLFVTDKGREFLENPTSF
                     MVLREHDYEGDNSDDDEDIITSKGGGVGFDEQLFQMLNDLRKTIAKQK"
BASE COUNT         2073 a         1438 c         1652 g         2187 t
ORIGIN      
        1 acacaatcat gtggttccga taaaacggaa gaatcttctg gtagtacttc gccaaaagtt
       61 gaggaagttt ccggttcgga ggatactgtt gtagattcag ttgctgcacc ggttgaagaa
      121 tctacgaggg atattacagt taatgaagaa ggagtaaaat aaaaaaggct gatgcattgc
      181 atcagccttt ttctatcggt taaaagaatt actttttgtc tttcacttct tcaaagtcga
      241 cgtctgtcac ctctgaatca cctgcacccg caccttggtt tcctgcatct gcaccgggag
      301 cgccgcctgt attggcgtac atctcttgcg cagccgattg aaacacctga ttgagttgat
      361 tcatggctgt ttcaatactt ggcatatctt tcgtttcata tgcttttttc agatctgcaa
      421 gggcagcttc tattggcgct tttttatctg ccggcaactt atcaccaaat tcattgagtt
      481 gcttttctgt ttggaagatc agactgtctg cagcattaag tttttcaaca gattcttttg
      541 cctgacgatc tgtttcggcg ttggcttctg cttctttgcg cattctgtca atctcttcct
      601 tgctcaagcc tgaacttgct tcaattctaa tcttttgctc tttgccagtt gccttatcct
      661 tggccgaaac atttaaaatt ccgtttgcat caatatcgaa tgttacctca atttgtggaa
      721 caccgcgttg tgccggtggt attccatcca ggttaaactg accgatagtg cgattgtcac
      781 gcgccattgg acgttcgcct tgcaaaacgt ggatcgttac agaaggttga ttatcggaag
      841 cggttgaaaa ggtttcagcc tttttggacg gaatagttgt attggcatcg ataagtctgg
      901 tcataacacc acccatggtt tcaattccaa gtgaaagtgg agtcacatcc aatagaagta
      961 catccttcac ttcaccggtt agaactgcac cttgaatggc agcacctact gcaaccactt
     1021 catcaggatt cacacctttg gatggttctt taccgaagaa gctttttact gcggcctgaa
     1081 cggcgggtat tctggtagaa ccccctacta gaataacttc atcaatatcg tttactgata
     1141 atccggcatt tttaagtgca gaacggcagg gttcgatagt gcgcttaaca agatcatcaa
     1201 ccagttgctc aaattttgca cgactaagtg ttcttacaag gtgctttgga attccgttaa
     1261 caggcataat gtagggaagg ttgatttcac tactgcttgt actggaaagt tcgattttag
     1321 ctttttctgc ggcttctttt aaacgctgaa gagccattgg atccttacgc aaatcgagac
     1381 cttcttcttt ttggaattct tctgccagcc aatcaataat tttctggtca aaatcatcac
     1441 cacctaggtg cgtgtcgcca tctgtggcct taacctcaaa tacgccatca cccaattcaa
     1501 gtacggatac gtcgtgtgtt cctccaccgc agtcgaaaac cacaattttc atctcctggt
     1561 ttttcttatc caatccgtat gccagcgaag cagcagttgg ttcattgatg atgcgacgaa
     1621 ccttaagacc ggcaatctca ccggcttctt ttgttgcctg acgttgtgcg tcgttgaaat
     1681 atgcgggaac agtgattaca gcctcggtta cctcgtgacc gagataatct tcagccgttt
     1741 ttttcatttt ttggaggatc atagccgata cttcctgcgg tgtgtacatg cgatcattaa
     1801 tcttcacacg cggtgtattg ttttcaccac gaaccacttc atacggaaca cgtgaaacct
     1861 ctttcgaaac ctcatcaaat gtattcccca tgaaacgctt gatggagtaa accgtattgc
     1921 gcggatttgt gatagcctga cgctttgcgg gatccccgat tttgcgctct cctccgtcta
     1981 taaaacctac aactgatggt gtagtgttct ttccttcgct gtttgggata acaacgggct
     2041 gattgccttc cataacggaa acacagctgt tggttgtacc caggtcgata ccgataatct
     2101 tgctcattgg atttgttttt ctgatttaac gcaatacact actcaaggct tgtgccaagt
     2161 gaagaagcag gaaaaaacag gaaaaaatgg cagaaaacac ccctccttaa gagtaaaaaa
     2221 cctgcagaat ggtcaggaaa tcaaggcaaa aaccgtcaat ccgactgtca gggacaataa
     2281 tacgtttcgg tcgaccatgg cagagaatca gtgcagaaaa gcaagtcgta ggtaaattga
     2341 cccgtgcaga gttccttctg cgagtcttta ttcaaaacct ttccgcgaac ccacttggtg
     2401 tatcggcttg aaaaaattcc aatcgcattg ctgagattgg taaacgaagg tctctcctgt
     2461 acgattcctg ttgatggttc atttaccttc atgtaggtat acatatcatc gcttactgca
     2521 acaaattgaa tgtccatttg acgcgcaaca cgtttaatta cgtttgcatt gcttggaatc
     2581 ttgagtttta catatcgata gaatgcgtct ccggcaaata ccgcaactct ttcaggctcc
     2641 gttccggctg tagatgatgt aatgtaggaa tctactttcc attcaatttt ttttctggtg
     2701 gttccgctgg ccgtatattc atcataccaa aaatgaaccc aggtgtctat tcggcgggtg
     2761 ttaattcccg gtgtaaatcg gatgtaataa tcggggtaat tgtttacaag tgaactgttg
     2821 ttgtaggcaa gagaaaaagg ataaagtgta ttccctgtta aagtattcag agggaagtca
     2881 tataccagtt ttgtagaacc tgtaacttct tttgcgccat cgttaatgtt tagatttagg
     2941 cggtattcgt aattctgatt tagggtggca atgaagtaat acatggtttg ctccggataa
     3001 taaaaggcac cggaatcgcg attggtaatt gttgtgtcct gcaggggata gctcgcaaca
     3061 agggtcccac tattccaggc ttcaacatta cctgaaacca atgcgtattc tgaagagtct
     3121 tttatagcgg cataatcgta tgcatttcct gtacccaaaa aggatttatt caccttgatg
     3181 aaatggatgc tttcgttttt gtcaagcaat ccaaatacaa tggtgttgtc tttgtatggg
     3241 ccaattatat caaaatcggt tgaacaagag ctgagcagcg caagagatac actaagtgca
     3301 acgaaaagtt ttttcataag acttcaaaaa taccaaaaaa agaacatctg atgtgtaccg
     3361 ttccccgttc cagtttatcc gttgatcaac cattcctgag tttgtttgat gatttttatc
     3421 ctaactttga caaaaacaag aatttatgtc ggcatcggct aaggaaataa agagggttac
     3481 tacgcatgtt ctgcatgaaa tgaagaacag aggggaaaaa atctctatgc taacggcata
     3541 tgattattct atggcccgaa ttgtggatga ttccggtatt gacattatcc tggtaggaga
     3601 ctcagcttcg aatgtaatgg cagggcatga aacaaccctt ccgattacac ttgatcaaat
     3661 gatttaccat gcttcgtcgg ttgtacgggc aatacaccgt tgtctggtgg ttgtggattt
     3721 accatttgga tcttatcagg gtaattcaaa agaggcacta aattctgcaa ttcgcataat
     3781 gaaagaatcg ggcgctcatg ccgtaaagct tgaaggtgga cgagaaatta aggaatcggt
     3841 tgaacggatt ctcaccgccg gaattccggt aatggggcat ttgggactta cccctcaatc
     3901 catttataaa tttggaacct acgttgtgcg cgctaaggaa gaagaggaag cagatcgact
     3961 gattcaggat gctcatttgt tggcggagtc tggttgtttt gctatcgttt tggaaaaaat
     4021 tcctgctaaa ctcgcttcaa gggtggcaaa agaagtaaaa gtcccaatta ttggtattgg
     4081 ggcaggacaa ggggttgatg gtcaggttct tgttgttcat gatatgcttg gtatcaacaa
     4141 tgagtttcaa cctcgatttt tgcggaaata tgcagacctg aaccaaatta ttggtgatgc
     4201 cgtgaaacag tatattgttg atgtaaaggc caaagatttt ccaaacgaaa aggaatcgta
     4261 ctagaattgg aattgcaggt tatttttgaa gacaatcacc taattgcggt aaacaagcaa
     4321 tccggagata ttgtgcaggg agataagacg ggtgatgtgc ccttgtctga agtaattaag
     4381 gaatacttga aagcgaaata caataaaccc ggaaatgttt tttgcggtgt gattcaccga
     4441 attgatcgtc cggttaccgg tgttgttttg tttgcaaaaa ccagcaaggc tttgagccga
     4501 atgtcggagc agtttagaga caagcaggta aaaaaaacct attgggcaat tgtaaaaaac
     4561 aaacctgctt cagaaaaagg aaatttggtt cattacctgc taaaagatga acgaaccaat
     4621 aaatccaaag ctttttccaa ggaggtaaag gatgcaaagt tttcggaatt gaattttaaa
     4681 ctgatcgcat ccggcgaacg ttatcattta ttggaggtgg atccacttac cgggcgacat
     4741 caccaaattc gggctcaact ttctgcaatg ggttgcccca taaaaggcga tcttaaatac
     4801 gggtcagaac gcagtaatcc tgatggttca atttcattac atgcgagaag aattgaattt
     4861 gtacatcccg taaccggaga acaacaaata atagaagcta ccgttcctga tgacagatta
     4921 tggaaggatt tggaaaaaat ggcaagtgca ggttgatctt caccttgctg ttggtgttaa
     4981 tcgcccaatt cggcctgagt caatcatctt ttttgaatta tttcagaatt agcaaccgtg
     5041 ccaatacagt gttgttatcc tgggaaatca gttcaggtaa tacttgcaat ggaacaagca
     5101 ttttgcgggg tgtagattcc ttgcagatga atgaagtagc tgccattgat ggtttatgtg
     5161 gggatcccgg atttgcggtt tcctacagct ttgccgattc cttccctcca ctaggacaga
     5221 aggtattcta ccgaattgat tttggtggat tggggttaag taaattactt gaaaccgtcg
     5281 tctacgattt aaccccgggg tattctgttt tatatgaaaa cgatggtttt cgcgttgttt
     5341 ttgccaatgt ttccggtagc attacgccct ttactttatt tgatctgacc ggacgtacaa
     5401 ttttaaaaag ttcaacaaat cagggttctt tttttgttcc ctatagccaa ttatccaccg
     5461 gagtagttgt atttgccgtg gaattgcctg aaggaaagtc gattcgggga aaaattgcga
     5521 tttcccgata aatccaattt tgcagaaatt ttcatcgaag gattcttagg aatcatagag
     5581 actttccaaa aaagtgtcta agtgtgcccg gaaaccagtt ttttttcata tatattagta
     5641 gggtaattat gagtttagac agtcaaggag aaaaacagta aatggaaaca atcgaaatat
     5701 cccccaaaag tgcattgcag aagttttttg gcttcagtaa atttaaggga catcaggaag
     5761 ctataattaa aaacctgctt gcaggaaatc attcgtttgt gattatgcct acaggtggag
     5821 gtaaatcatt atgctatcaa ttacctgctt taatgatgga ggggacggct gttgttgttt
     5881 ctccgcttat tgcattaatg aagaatcagg ttgattcaat acggacgttt ggaagtgatg
     5941 acagtgtagc tcacttttta aattcttccc tcagtaaggc ggagatttta aaagtgaaaa
     6001 agaacataat ggaaggccgt actaagcttc tgtatgttgc gcctgaatcg ttaacgaaac
     6061 aagagaacat cgattttctc actacgataa aagtttcatt tttcgctatt gatgaggcac
     6121 attgtatttc cgaatgggga catgatttcc gtccggaata ccgtaagctg cgaccgatga
     6181 ttgatcgtgt tggtgaaaat gtacccatta ttgcactcac tgcaactgca acaccgaaag
     6241 tgcagctgga tattcagaaa aacctgggta tgcaggacgc tactttgttt aaggcttcgt
     6301 ttaatcgcga taaccttttt tatgaagttc gtccaaagcg cgatgttgta aaggagattg
     6361 tacgttttat caagaccaag caaggaaaat ccgggattgt atattgtttg tcccggaaaa
     6421 aagttgaaga gcttgctgag gtactccgtg taaatggaat caaggcattg ccatatcatg
     6481 cggggctgga tgcttcaaca cgtgcaaaac atcaggatat gttcctaatg gaggatgcag
     6541 atgttattgt ggccaccatt gcatttggga tgggtatcga caaaccggat gtgcgttatg
     6601 taattcacca cgatatccca aaatcacttg aatcatatta tcaggaaaca ggacgtggcg
     6661 gacgagatgg aggagagggt cattgccttg cattttatag ttacaaggat gtagagaaat
     6721 tggaaaaatt tctcaatgga aagcatgttt ctgaaattga aattggcaag caactgttgc
     6781 atgaaatagt ttcttttgcg gaaacctcaa tgtgtcggag aaaatatttg ttacactatt
     6841 ttggggaagg atttaaagaa gaagattgcg gaaagatgtg cgacaattgc cgcaatccac
     6901 gcgaaaaaac ggaaggtaaa gatgaggttg ctcttttgct cgaagcaatt cttgaaacga
     6961 aagaaaaaca caagccttcg catattgtaa gcatcttact gggtgaactt acctctgatg
     7021 tgcgaacgta caaacacaat aagctcgagg ttttcgggaa aggttcagag agtacagatc
     7081 gtttctggaa tgccgttgtt cgtcaaaccc tcgttgccgg gttgattcga aaggatattg
     7141 aaacttacgg tgtattgttt gttaccgata agggacgcga atttctggag aatccaacat
     7201 cctttatggt gcttcgcgag cacgattacg aaggcgataa ctccgacgat gatgaggata
     7261 tcattacatc aaaaggtggt ggagtcggtt tcgatgagca gcttttccag atgctaaatg
     7321 atttgcgcaa aacaatagca aagcaaaaga
//
LOCUS       DAOMYG010000115         7282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3434_length_7282_cov_9.781652, whole genome shotgun sequence.
ACCESSION   DAOMYG010000115 DAOMYG010000000
VERSION     DAOMYG010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7282
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3434_length_7282_cov_9.781652"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..939
                     /locus_tag="PK610_05100"
     CDS             136..939
                     /locus_tag="PK610_05100"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRJ35340.1"
                     /translation="MKTRIALMSLVLSGSMLVAKANLQNGENQFRKVNNQSFKPGEVL
                     RYRLHYGFVDAGEATLRVKECDRTMNGRKMIHVEGVGTSISAFDWFFKVRDRYESYLD
                     AEGIFPWLFIRRVNEGGYEINQDYSFMQHKQKVDNGKGKTFETPAMVQDMISAFYYAR
                     TLDFTNAKPGDEFMIPMFYDDTNFPLKIKFVKRETINVRMGKFKCLKFAPVVEKGRVF
                     KSEEDLAVWVTDDVNKIPLLAKAKILVGSIKMEVVETKNLAGPISKIEK"
     gene            1093..2373
                     /locus_tag="PK610_05105"
     CDS             1093..2373
                     /locus_tag="PK610_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRJ35341.1"
                     /translation="MKKLQSLLLIFICCLTLFMVSCDEKQKDEAGSVRSVSPNKLPEI
                     EYGFLLDTFHVVRDKVKGGMTLSHVLAPHGISQQHINLTDQKARSDAKMTFISEGHRY
                     TLLKNKKDTTKTHVFIYEKNRIEYVIFRFEKDVTAQLIQRPVSVVEREMSGIIEKNSN
                     LVFTIKEQLNDDAVAGELAETIAQVFAWTIDFFKLYPGDEFRVVYEEKQVEGVPYSIG
                     KIKAAYFYNISAPYYAFRWEQNGEIGYWDENGKGMKRPFLKAPLKFSRITSGYSSNRL
                     HPVQKVWKAHLGTDYAAPTGTPIMATADGTVEAAGYSGGNGNYVKLYHNKTYQTGYLH
                     MSKIESGMKKGVRVKQGEIIGYVGSTGLATGPHVCYRFWKNGSQIDPRGEKFQSTEPI
                     KESDMGRYKEYILPLRSRLDSIVAKASPQKDSLQ"
     gene            2370..3728
                     /locus_tag="PK610_05110"
     CDS             2370..3728
                     /locus_tag="PK610_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35342.1"
                     /translation="MKHFLLLLHTICITYAIAQSPCSTPENYIPSGDEPEVIVRVKLH
                     VVQYSKTDPRNYTEKDTAALKQQFDLINLFYNEMEAPTLKPTKEVDHLRSARIRFQVV
                     EIRFHQDERLWDRIGLHPAENRQLPAAIDSISSGRKEIWIAGNHMYRIGNKKAQFRRE
                     GMQHLDMQFDTAFFHREKKMTKIIFRESVPVEYAQGKITFYMEEDKNCHDDLFHNLAQ
                     SDSSYLHVFYTGASARKMAFGCGPSPYYLNVSNYTNGGEWANAQLSAHELGHCVGLSH
                     TDFPQFDDLPKKDKFGFIDCDSIEVSNNIMGYNKCRRYLSPKQIAWVHQEYSLKPYRI
                     RTTAYCTYNPQYTWTIRKNTTWNRAMVIGGDLVIRKGKTLTVNCMVSLPEGAHIILEE
                     GAKLILNGNVITNNCGGTWNGVLYCRKFSGDKSRLKPVKKKKGNVQLQNGGRFERMQQ
                     QN"
     gene            complement(3777..5246)
                     /gene="cysS"
                     /locus_tag="PK610_05115"
     CDS             complement(3777..5246)
                     /gene="cysS"
                     /locus_tag="PK610_05115"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015362726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRJ35343.1"
                     /translation="MEHPLNVYNNLTRSKERFVPIQSPFVGMYVCGPTVYNYMHLGNL
                     RTFITFDMVYRYLVHLGYKVRYVRNITDVGHITTEFDEGVDRIGAQAKLEQLEPMEIV
                     QKYTNNFHDVLNQFNCIPPSIEPTATGHIIEQIEMVKQILENGFAYEADGSVYFDVRA
                     YMQKHKYGALSGRNVDELFENTRELDGQDEKRFFADFALWKKASPEHIMKWPSPWGEG
                     FPGWHLECSAMSTKYLGEEFDIHGGGMDLKFPHHESEIAQNTACCGHGPVRYWLHGNM
                     LTVNGEKMSKSKGNSFLPLELIAGSHPLLDQGYSEMTVRFFMMQSHYASTLDFSNDAL
                     KAAEKGYKRFMTAIDALEKVKAGTASTFSVDDLRRDCYAAMNDDFNTPILIAGLFECV
                     KQINLMLEGKAALTDADLSDFKSMVHAFVFDVLGFRRENESAGNTKALDAAMQLVIEM
                     RRKARENKDWTTSDTIRDQLKAAGIVIKDTKEGVDWMLE"
     gene            complement(5359..6228)
                     /locus_tag="PK610_05120"
     CDS             complement(5359..6228)
                     /locus_tag="PK610_05120"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HRJ35344.1"
                     /translation="MRNLLLKNLSVLMFAISSMVASAAAGQGDLNQTDANGWKQGHWV
                     ITASMKATPGFKPDQVVEEGDYKDNKKTGMWKMYYPNGNVKQEAEYKNNRPSGKYTNY
                     YESGKVEEEGNWKNNANTGNFKRYHPNGQVAQEKTFTESGKTTGKVVYYFPNGKKELE
                     YGSNNGVEDGEMTRYYPNGDVKEKKNFSGGKVDAGSEKEFKMVNPPVKLEEEPSKVAT
                     TDPNLKANEADVKVKDGYNKLYDDQKRLVQDGEFKGGKLFNGKWYKYDKNGLLLKIEV
                     YKNGKYVGDGQIE"
     gene            complement(6386..>7282)
                     /locus_tag="PK610_05125"
     CDS             complement(6386..>7282)
                     /locus_tag="PK610_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRJ35345.1"
                     /translation="ELVFELRRIYGSDQVVSCDLKEDDKTDPLYEQADALDKERILAI
                     VKKHQITQIYLLAALLSATAEKNPAFAWKLNMESLFHVLEMAKEGIIKQIYWPSSIAV
                     FGPTTPREHTPQYTVMEPSTVYGISKQAGERWCEYYHKKFNVDVRGLRYPGLIGYKSL
                     PGGGTTDYAVDIFHQALKSGSYECFLEADTALPMMYMEDAIRATIEIMQAPTEKIKIW
                     SSYNLAGISFTPDQIAGAIRKHIPSFTITYQPDFRQAIADSWPKSIDDHAAQSDWGWK
                     AAYDLDTMVETMLKNIPQFWKL"
BASE COUNT         2103 a         1643 c         1508 g         2028 t
ORIGIN      
        1 ggaaatctaa gcaggccatc ggaaaaacaa cgcgaacagg tcattgattt tcaacgtgtt
       61 gaaaaatgat agctccggaa ataacatttg gaacgctttt tgcgttttta ccaacaagaa
      121 ccaaaagaaa agcctatgaa aacccgaatc gccctgatga gtcttgtatt gagcggaagc
      181 atgctcgtag caaaggccaa tctccagaac ggggaaaacc agttccgtaa agtgaacaac
      241 caatccttta aacccggaga agtattacga taccgtctgc actacggatt tgtagatgca
      301 ggggaagcaa ccttgcgggt taaagaatgc gaccgcacca tgaacggaag aaaaatgatt
      361 catgttgaag gtgtgggaac ttccatttcg gcattcgatt ggtttttcaa ggtgagagac
      421 cgttacgaat cgtatctgga tgcagaaggt atttttccct ggttgtttat ccgcagagtg
      481 aatgaaggtg gttatgagat caatcaggac tactcgttca tgcagcacaa acaaaaagtg
      541 gataatggca aaggaaaaac atttgaaact cctgctatgg tgcaagatat gatctcggct
      601 ttttactacg ccagaacgct tgattttacc aacgcaaaac ccggcgacga attcatgatt
      661 ccgatgtttt acgacgacac taattttccg ctcaagatta aattcgtaaa aagagaaacc
      721 atcaatgtgc gcatgggaaa attcaaatgc ctcaagtttg cacctgtcgt tgaaaaagga
      781 cgcgttttca aatcggaaga agatctggct gtttgggtaa cggatgatgt aaacaaaatc
      841 ccgctgcttg caaaagcaaa aatcctggta ggctccataa aaatggaagt ggtagaaaca
      901 aaaaatcttg ccggacctat ctccaaaatt gaaaaataaa cgatgtttca tacctgggaa
      961 aagtcctgct taaaacagcg ggactttttc atttacaccg acgcagataa ttcttcacat
     1021 gctactctta ataaatcctt tcagcgcaaa acatcaattc tctacctttg acagaaccaa
     1081 acccaacctg ttttgaaaaa attacaatca ctccttttga tttttatctg ttgtctgacc
     1141 ctttttatgg tttcatgcga tgaaaaacaa aaagatgaag caggttctgt acgcagtgtt
     1201 agtccgaaca aactccctga gatcgaatac ggttttctgc tcgatacttt tcatgttgtt
     1261 cgtgataaag taaaaggagg aatgacctta tcgcatgttt tggctccgca tggtatcagt
     1321 cagcaacaca tcaaccttac cgatcagaaa gcaagaagtg atgcaaaaat gactttcatt
     1381 tcggaagggc atcgttatac tttactgaaa aacaaaaaag acactacaaa aacacatgta
     1441 ttcatttacg aaaaaaacag aattgaatat gtgatcttcc gctttgaaaa agacgttact
     1501 gcacaattga ttcagcgtcc ggtttcggtt gtggaaagag agatgtcggg catcattgag
     1561 aaaaattcca atctcgtttt taccattaag gaacagctca acgatgatgc cgttgcaggt
     1621 gaactggcag aaacgattgc acaggtattt gcgtggacca tcgacttttt taagttgtat
     1681 cccggagatg aattcagagt ggtttacgaa gagaaacagg tagaaggcgt tccctattcc
     1741 attggaaaaa ttaaagcagc ctatttctac aatatcagcg caccctatta tgcttttcgc
     1801 tgggaacaaa acggagaaat cgggtattgg gacgagaatg gaaaaggaat gaaacgtcca
     1861 ttcttaaaag caccacttaa attctcgcgc atcacttccg gttattcatc caaccgattg
     1921 catcctgtac aaaaagtctg gaaagcgcat cttggcaccg attacgctgc accaactgga
     1981 acccccatta tggccaccgc cgacggaact gtagaagccg caggatacag tggtggaaat
     2041 ggaaactacg tgaaattgta ccacaacaaa acctatcaga ccggctatct gcacatgagt
     2101 aaaattgaaa gcggaatgaa aaaaggtgtg cgtgtgaaac agggagaaat catagggtat
     2161 gttggatcca ccggactcgc aaccggccca catgtttgct accgtttctg gaaaaacgga
     2221 tcacagatcg atccccgcgg cgagaaattt caatcaacag aacccattaa ggaaagcgat
     2281 atgggtcgct ataaagaata cattctcccg cttcggtcgc gactcgattc tattgttgca
     2341 aaagcatcac cacaaaagga cagtttgcaa tgaaacattt tctgctgctg ctacatacca
     2401 tctgcattac ttatgcaata gcacaaagtc cgtgcagcac accagagaac tacattccaa
     2461 gcggagatga gcctgaagta attgttcgcg taaaacttca tgtggtacaa tattccaaga
     2521 ctgatccgcg gaactacacc gaaaaagata ccgcagcttt aaaacaacag tttgatctga
     2581 tcaatctttt ctacaacgaa atggaggctc caacgctcaa gccaacaaag gaagttgatc
     2641 atttgcgaag tgcgcgtatc cgatttcagg tagtggagat tcgatttcat caggacgaaa
     2701 ggttatggga tcgaatagga ttacatcctg cagaaaaccg acaacttccg gcagcaattg
     2761 attccatttc ttccggtaga aaagaaatat ggatcgcagg caaccatatg tacagaattg
     2821 gaaataaaaa agcacaattc cgaagagagg gaatgcaaca cctcgatatg caattcgata
     2881 ccgccttttt tcacagggag aaaaaaatga caaaaattat tttccgcgag tctgttcctg
     2941 ttgaatacgc gcaaggcaaa atcactttct acatggaaga agacaagaat tgtcacgatg
     3001 atctattcca caatcttgca caatccgaca gttcctattt gcatgtattc tacaccggcg
     3061 cctctgcacg taaaatggca tttgggtgtg gcccttcacc ctattacctc aacgtatcca
     3121 attacaccaa tggaggagaa tgggccaacg cacagctatc tgctcacgag cttggacact
     3181 gtgtgggatt atcacatacc gattttccgc agttcgatga tcttcctaaa aaagacaaat
     3241 tcggtttcat cgattgcgac agtattgagg tgtcgaacaa cattatgggt tataacaaat
     3301 gccgcaggta tttatcgccc aaacaaattg cctgggtaca tcaggagtat tcgctaaagc
     3361 cctatcgcat tcgcaccaca gcatattgca cctacaatcc gcaatacacc tggaccatcc
     3421 gcaaaaacac aacatggaac cgcgcaatgg taattggtgg cgatctggtc attcgcaaag
     3481 gaaaaacgct cacggttaat tgcatggttt cccttccgga aggagcgcat atcattttag
     3541 aagaaggagc aaaactaatc cttaacggca atgtaatcac caacaattgc ggaggcacat
     3601 ggaatggcgt gttgtattgc agaaaattca gcggcgataa atcacgacta aaacccgtta
     3661 aaaagaaaaa aggaaatgtc cagcttcaaa acggaggaag attcgaacgg atgcaacagc
     3721 agaattaaat ttcttctgca aaacaaatag tgcattacag aaaaatacac tgtcgattat
     3781 tccagcatcc agtctactcc ttctttggtg tcctttataa caatacctgc tgcctttaac
     3841 tgatcacgaa tagtatcgct tgttgtccag tctttatttt cacgggcttt tctgcgcatt
     3901 tcaataacca attgcattgc tgcatcaagt gcttttgtgt ttcctgccga ctcattttca
     3961 cgtcgaaaac caagaacgtc gaaaacaaaa gcatgcacca tcgatttaaa atcagaaaga
     4021 tcagcatctg tcaatgctgc ttttccttcc agcatcaaat tgatttgttt tacgcactca
     4081 aacaatcccg caatgagaat gggtgtatta aaatcatcgt tcattgctgc ataacaatcc
     4141 cttctcaaat catccacact aaaggttgaa gctgttccag ctttaacttt ttcaagtgca
     4201 tcgatagcag tcataaatcg tttatatcct ttttctgcag cttttaatgc atcgtttgaa
     4261 aaatcgagtg ttgatgcata atgcgattgc atcatgaaaa aacgaacggt catttccgaa
     4321 taaccctggt cgagtaatgg atgagatcca gcaatcagct ccaacggaag aaatgaattg
     4381 cctttcgact tactcatttt ttcgccgttt acggtaagca tatttccatg aagccaataa
     4441 cgcacaggac catgtccgca acaggcggta ttttgcgcta tctccgattc gtgatgcggg
     4501 aatttcagat ccattccacc gccgtgaata tcgaactcct caccaaggta ttttgtgctc
     4561 atggctgaac attccaaatg ccaacccgga aatccttctc cccagggcga aggccatttc
     4621 ataatatgtt caggtgaagc ttttttccat aaggcaaaat ccgcaaaaaa acgtttctca
     4681 tcctgaccat ccaattcgcg cgtgttctca aataactcgt ccacatttct tccgctcaag
     4741 gctccgtatt tgtgtttttg catataggca cgaacatcaa aatacaccga accatcggct
     4801 tcgtaggcaa aaccgttttc aagaatctgt ttcaccattt ctatctgttc aatgatatga
     4861 ccggtagcag tgggttcaat acttggagga atacaattga actgatttaa tacatcgtga
     4921 aaattattgg tgtacttctg cacgatttcc attggctcca attgctcaag cttggcctgc
     4981 gcaccaattc gatctactcc ttcatcaaat tcggtggtga tatgacccac atcggtgatg
     5041 ttgcgaacgt acctaacctt gtagcccagg tgaaccaggt agcggtaaac catatcgaac
     5101 gtaatgaacg tacgcaaatt accaaggtgc atgtaattgt aaacggtagg accacaaaca
     5161 tacattccga caaaaggcga ttgtatcggc acgaatcgtt ctttggatcg tgtgaggtta
     5221 ttgtagacgt tcaagggatg ttccatgagg taaaaataca cactattttg agcccgctaa
     5281 aacgagtttt tgcatttgcc ctaaaacaaa aactcccaac catcaggccg ggagtttttt
     5341 gtccgattgc tttgctcctt attcgatctg tccgtcgccg acgtacttac catttttgta
     5401 tacttcgatc tttaagagaa gtccgttctt atcgtacttg taccatttac cgttgaacag
     5461 tttgccacct ttgaactcac cgtcctgcac aaggcgtttc tgatcatcgt aaagcttgtt
     5521 gtatccatct ttcaccttca catcagcctc attggctttc aggttaggat cggtggtagc
     5581 aactttgctt ggttcttctt ccagtttaac aggaggattt accattttga attctttctc
     5641 agatccggca tctacttttc cgccgctgaa attctttttc tcttttacgt cgccattagg
     5701 ataatagcgc gtcatttctc cgtcttccac cccattattg gatccgtact caagttcctt
     5761 tttaccattg gggaagtaat acaccacttt acctgtggtt tttccgctct cggtaaaggt
     5821 tttttcctga gctacttgtc cgttaggatg gtagcgcttg aagtttccgg tgttggcgtt
     5881 gtttttccag ttaccttctt cttccacttt tccactttcg tagtagttgg tgtatttacc
     5941 ggaagggcgg ttgtttttgt attcggcttc ctgcttgacg ttaccgttcg ggtaatacat
     6001 cttccacatt ccggttttct tattgtcttt atagtcgccc tcttccacta cctgatccgg
     6061 tttaaacccg ggtgtggctt tcattgacgc tgtaatgacc caatggcctt gtttccagcc
     6121 gttggcatct gtctgattta aatcgccctg tccggcggca gcagaagcta ccatggagga
     6181 gatggcgaac atcaggacac tgagattttt gagtaggagg ttccgcataa ttctcaattt
     6241 tatcctctga agcaatttac gtcatttctt ttccaaggtt gcctgcattt cgcccatcgg
     6301 ggggtattcg tcgacaaacc ggggtattaa aaggacgtta agttctcttc tgaatggttt
     6361 aaatatagtt acgatttcaa cattttcaaa gtttccaaaa ttgggggatg tttttcaaca
     6421 tcgtttcaac catggtgtcc aaatcgtatg ccgctttcca tccccagtcg gattgagcgg
     6481 catgatcatc gatactcttt ggccaggaat cggctatagc ttgcctgaaa tcaggttgat
     6541 acgttattgt aaaggaagga atatgcttgc gaatggcccc tgcaatttgg tcgggagtga
     6601 agcttatccc tgccagattg tagctcgacc atatttttat tttctctgtt ggagcctgca
     6661 tgatctcgat ggttgcccgg atggcatcct ccatatacat cattggcaag gcggtatccg
     6721 cttccaaaaa acattcgtac gaacctgact ttaaagcctg atgaaaaata tcgaccgcat
     6781 aatccgttgt tccgccaccg ggcagcgatt tgtatccgat caaaccgggg taacgcagtc
     6841 cccgcacgtc tacattgaac tttttgtggt agtattcgca ccaccgctca ccggcctgtt
     6901 tggaaatacc atataccgta ctgggctcca ttacggtgta ctgcggagta tgttcgcggg
     6961 gcgtggttgg accaaaaaca gcgatggagc tgggccaata gatctgtttt atgattccct
     7021 ctttcgccat ttcaagaaca tgaaagagac tttccatatt cagcttccag gcaaaggcag
     7081 gatttttttc tgctgtggca gataagagtg ctgcaagtaa atagatctga gtgatctggt
     7141 gtttttttac aatggcaaga atacgttctt tatccagcgc atctgcctgc tcgtacaggg
     7201 ggtcagtttt atcgtcctct tttagatcgc aggaaacaac ctgatcgctg ccatagattc
     7261 ggcgcaattc aaaaaccaac tc
//
LOCUS       DAOMYG010000116         7245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3455_length_7245_cov_11.678164, whole genome shotgun sequence.
ACCESSION   DAOMYG010000116 DAOMYG010000000
VERSION     DAOMYG010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7245
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3455_length_7245_cov_11.678164"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..4102)
                     /locus_tag="PK610_05130"
     CDS             complement(17..4102)
                     /locus_tag="PK610_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ35346.1"
                     /translation="MKHLINRLLFVTVVTGISLISNTSKASHVPGGNITYECIGPNQF
                     AVTLTLFEDCGTAFEGNFNQTLQVSNSCGLANPTLSLPNVIYQQEVSQLCPQQIGQSE
                     CSGGSLPGVYMHQWTGIITLPAGCDSWTFAYSSCCRNTAVNSTFQDSYYWSATLNSTT
                     APCNNSAQITSQPIPYVCINQQVNFNMAAIDPDGDNLVFSLVPAMTTGPATTITYAGG
                     YSGASPINGITINPSTGQITFTPNILGNFIVAVLIQEYDANGNLTGTVVQDFQFEVIN
                     CVNQVPAVPVGGITNYTGGGAQTGPNSIQVCEGDNFCFNLTFTDTDVANTLQVTSNIQ
                     QIFPGATITSTGTNPVTIQVCHTVPSGAPANSIISFEVEDNACPIPGVNSFPITVTVI
                     NSTYAGMDQIMCLNQGAQLNANGGSNFNWTVLSGAPITIGTNFSCNNCPNPIANPNIT
                     TTYVVTSNLSGGCTNVDTVTINVVPDFSFVINQASPTSCLQDPINISVVTTPAGAFTY
                     NWTPATYLSSTTSPNVVVNATAPGTYNYTVTITSPGGCVKTGTTSLVVFPAMSPTPTA
                     SASPNMLICGGGVTQLSANLGGGVPATCGPSTSGGCSGPTTAINPGTQSGSNTTTSYP
                     AIWGNWYKSSHTQMIFTAAELNAMGFMGGKITRLNIPVTQINGTTLYKNVTVMMGCTS
                     QSQYTGQTWITALTPVFGPANVPIAVGNNYITLSTIYEWDGISNLVVDICSDNTSDAS
                     WTQNSISAQTTTAQPRCQFLYQDFQSTCGFTQLPNFASPSTIRPKIEFTTCPSVPDTT
                     QFSYLWTPSANVVNPTSLTTNASPTQTTNYTVLVTNNNGGCTATASVQVIVDCLCYPA
                     NPAITNVSCNGGSNGAITATMVGTTGPWTVNWYNSAGVLLQTTPNVNTTDILTGLPAG
                     TYTIETIDTALCARDTTITITQPTIVTGNAGTDAVICIGNTTTLSATATGGTAPYTFA
                     WSNGATGSSNAVTPAANTCYDVIVTDANGCTSVADQVCIFLNPPIIATAMNDATICPG
                     ASIALTATATGGNGGPYTYVWTLNGNNVGTGTPLNVTPPANGSQYCVSVTDNCGTPAG
                     TDCVIINFEVVPTPTFTADNLESCIPLITNFTNTTDPSLYTTATWNFGDGNSSTTQPT
                     VGHSYPVPGCYDVTLSLETAIGCTATTTLPSYVCARPYPVASFLFGPQPTNLFAPDIT
                     FISNSTPDAITFFYDFAGLGTSTLPATTFSFPNDNPGNYPVTFAVSNQFGCTDTIVET
                     IIINGIYTIYAPNAFTPDGDGRNDIFFVTGEGFDPTRFEFFIFNRWGEVIFQSESAAN
                     GWNGMHNGQKAKNDVYVWKIKSKDQWTGQKFETYGHVTLIR"
     gene            4252..4857
                     /gene="coaE"
                     /locus_tag="PK610_05135"
     CDS             4252..4857
                     /gene="coaE"
                     /locus_tag="PK610_05135"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRJ35347.1"
                     /translation="MKDSNNREITIGLTGNIGAGKTTIAAVFQEFGIPVFDADKVGRR
                     LLDTDQDIKNGLIKIFGKDILTNNLPDRKKIANLVFSDDSLLIELNKLIHPRVGEVFR
                     EWRKNQMAKVVIREAAILIESGTFRDLDILIVVTCPENIRLKRVMERDHSNESEVRAR
                     MNKQMNEEEKVALADFVIINDGKTLVIPQVLDVLQKLKINN"
     gene            4867..6408
                     /locus_tag="PK610_05140"
     CDS             4867..6408
                     /locus_tag="PK610_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRJ35348.1"
                     /translation="MIIPDPDQLKLLDKRTIEIQGIRSLDLMERAALKWLDEFLNDLN
                     LISRVGKGSGDQTIHIFCGPGNNGGDGLVVARHLMIQGFSPIVYVLNIGKKPTEDHLE
                     NRKKAEQKGITIIDLKESAQLPEFRENDCVIDALFGFGFVGKLEGFAAELVEHINTSN
                     TRIYSIDIPSGLPSNENPEGKTIVRASVTFTFHSPKLSFFFPENAQYTGRWKVVDIGL
                     EWSELKADRNAVHFILPESLAVFLKPRAKFSHKGDYGHALLLAGGRGKVGAAQLSGCA
                     ALKSGCGLLTIATPLCGEIPLQTALPEAMVIADAENDFISMLPHLDKYDAIGAGPGIG
                     LDKQTGNVIKLLIQQTQVPLVLDADALNILAENKTWMSFLPAGTILTPHPGEFDRLFG
                     RHTSGYDRYITLKEKAQKHNVIIVLKGAYTCIAHPDGSTWFNSTGNPGMAKGGSGDVL
                     TGVILGLLARGYSPFQATMIGVWIHGKAGDLCLITFGQESVLASNICEKIVDAFSSLE
                     RRHYY"
     gene            6515..7225
                     /locus_tag="PK610_05145"
     CDS             6515..7225
                     /locus_tag="PK610_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRJ35349.1"
                     /translation="MDISKVRILVSAIQKLSLARDLDRIMDIVRRSARQLVGADGATF
                     VLKEGEKCYYADEDAISPLWKGQKFPISACISGWCMINKKFVVIEDIYNDERVPIEAY
                     RPTFVKSLAMVPIRTINPVGAIGVYWAKLHSPTDEDVEVLQSLADITSVSIENVFVYE
                     ELKEQNKILYDIAFLQSHQVRVPVAQLKGLFELINFDDLSFEKNSEIFERIKITVDSF
                     DKTILEITEMSSKIKYYQ"
BASE COUNT         2123 a         1283 c         1704 g         2135 t
ORIGIN      
        1 tgtttatagg attatattat ctgatcaaag taacatgacc gtatgtttca aatttttgtc
       61 cggtccactg atcttttgat ttgattttcc aaacgtaaac gtcgtttttc gctttctgac
      121 cattatgcat tccgttccac ccgttcgctg cactttctga ttggaaaatt acttctcccc
      181 aacggttgaa gatgaagaac tcaaaacggg taggatcaaa accttctcct gttacaaaga
      241 agatgtcatt tctgccatcc ccatccggag taaatgcatt tggcgcatag atggtgtaaa
      301 ttccattgat gatgatggtt tctacaattg tatccgtaca tccaaattgg ttacttaccg
      361 caaaggttac cggatagttg cccggattat cattcgggaa actaaatgtt gttgcaggta
      421 gtgtagaagt tccaaggcct gcaaaatcat agaagaatgt aattgcatcc ggcgttgaat
      481 tgcttatgaa cgtgatgtca ggcgcaaaca gattggttgg ttgtggacca aagaggaagc
      541 tggcaacagg gtatggacgt gcacaaacat agctgggcag tgttgtggtt gctgtacatc
      601 caattgctgt ttccagtgaa agcgttacat cataacaacc cggtacagga taagaatgtc
      661 caacagtagg ttgtgtagtt gatgaatttc catcaccaaa attccaggtt gctgtagtgt
      721 aaagtgaagg atctgttgta ttggtaaagt tggtgattaa aggaatacaa ctttccagat
      781 tgtcagcagt aaatgttggt gtgggtacca cttcaaagtt gataattaca caatcagttc
      841 ctgcaggggt tccgcagtta tcagttacac ttacacagta ttgagatccg tttgctggag
      901 gagttacatt taatggtgtt ccagttccta cgttattgcc attaagagtc cagacgtagg
      961 tataaggtcc gccatttcca cctgtagctg ttgcggttaa tgcaatggat gcacctggac
     1021 aaattgttgc atcattcata gcagttgcaa tgatgggagg attgaggaag atacaaacct
     1081 gatcggcaac tgaagtacat ccgttagcat ctgttacgat tacatcataa caagtatttg
     1141 ctgcaggggt aacagcattt gaagatcccg tggcaccatt cgaccacgca aaagtgtatg
     1201 gagccgtccc tcctgttgct gttgcagaaa gtgttgtcgt gttgccgatg caaattactg
     1261 catcagtacc tgcattaccc gttacaatag ttggttgagt aattgttatt gtagtatctc
     1321 ttgcgcaaag tgctgtatca atagtttcaa ttgtataggt tcctgccggc agaccagtta
     1381 aaatatcggt agtatttacg tttggagtag tctgcaaaag tactccggca ctgttgtacc
     1441 agtttactgt ccatggaccg gttgttccaa ccatagttgc cgtaattgca ccattgctac
     1501 ctccattaca acttacgttg gtgattgcag ggtttgcagg gtagcacaaa caatcaacaa
     1561 ttacctgcac gcttgctgtt gcagtacatc cgccattgtt gttggttaca agaacagtat
     1621 agtttgtggt ttgcgttgga cttgcattcg tagttagaga agttgggttt actacgttag
     1681 cgcttggtgt ccataaataa ctgaactgag tagtatccgg tacggatgga caagtagtga
     1741 attcaatttt tggtcgaatg gttgaaggtg atgcaaaatt cggcaactgc gtaaatccgc
     1801 aagtactttg aaaatcctga tacaggaatt gacaacgggg ttgcgctgtt gttgtttggg
     1861 cggaaatact gttttgtgtc caagatgcat ccgaagtgtt atctgaacag atatcgacaa
     1921 cgagattaga gataccgtcc cattcgtaaa tggtagacaa agtaatgtaa ttgttcccga
     1981 ctgcaatggg aacgtttgca ggaccaaaaa caggagtgag agccgtaatc caagtttgac
     2041 ctgtgtattg actctgactt gtacagccca tcataactgt gacgttttta taaagtgtag
     2101 ttccgttgat ttgagttacc ggaatgttta aacgagtgat tttaccaccc ataaatccca
     2161 ttgcgtttaa ctcagctgct gtaaagatca tctgagtatg cgacgattta taccaattcc
     2221 cccaaatggc agggtagctt gttgttgtgt tggatccaga ttgagtcccg gggtttatag
     2281 ctgttgttgg tcctgaacat ccgcctgagg tactcgggcc acaagttgca gggactccgc
     2341 cgccaaggtt tgcagaaagc tgggtaaccc cgccaccgca aattaacatg tttggactag
     2401 cagatgctgt tggcgttggt gacattgcgg ggaaaacaac gagggaagtt gttccggttt
     2461 ttacacatcc acctggagaa gttatagtaa ccgtataatt atatgttccc ggtgcagtag
     2521 cgtttacaac aacattagga cttgttgttg aacttaggta tgtggcaggg gtccaattgt
     2581 atgtaaatgc cccagcaggg gtggtaacaa ccgaaatatt gattggatcc tgcaaacatg
     2641 aggttggaga agcctggtta atgacaaaag agaaatcagg aacaacgtta attgtaactg
     2701 tgtccacatt ggtgcaacca cccgataaat tactggtgac aacatacgta gtggtaatgt
     2761 ttggatttgc gatggggttc ggacagttat tgcaactgaa gtttgttcct atcgtaattg
     2821 gtgcacctga tagaacagtc cagttaaagt tagatcctcc gttagcattc agctgcgcgc
     2881 cctgattcaa acacatgatt tgatccattc ccgcataggt agaatttatt actgtaacag
     2941 tgataggaaa ggagttcact ccgggaatag gacaagcatt atcctcaacc tcaaatgaaa
     3001 taattgagtt tgcgggtgca cctgatggta cggtgtgaca aacctgaata gtgacgggat
     3061 tggttcctgt tgaggtaata gtcgcccctg ggaaaatctg ttgaatgttg gaagttacct
     3121 ggagagtgtt ggccacgtca gtgtctgtaa atgttaggtt gaaacaaaag ttgtctcctt
     3181 cacaaacttg tatactgttc ggtccggttt gtgcaccgcc accggtgtag tttgtaatac
     3241 ccccaacagg aactgccgga acctggttta cacaattgat aacctcaaac tggaaatcct
     3301 gaacaactgt tcctgttaga tttccgtttg catcgtattc ctgaattaag acagcaacaa
     3361 taaaattccc aagaatattt ggtgtaaagg tgatctgtcc ggtagaagga ttaatagtta
     3421 tcccgttaat tggacttgct cctgaatacc caccagcgta ggtgattgta gttgcaggac
     3481 cggttgtcat agcagggaca agtgaaaaca ccagattgtc tccgtctgga tcaattgcag
     3541 ccatgttaaa gttcacctgc tggttgatac atacgtaagg aataggctgc gaggtaatct
     3601 gagcagaatt attacaaggc gctgttgtac tgtttaatgt agcagaccaa taataggaat
     3661 cctgaaaggt cgaattcacc gctgtattac gacagcagga tgaataagca aatgtccagg
     3721 agtcgcaacc ggcaggaaga gtaatgatgc ctgtccattg gtgcatatat accccgggaa
     3781 gactaccgcc cgaacattca gattgtccga tttgttgcgg acacagctga gaaacctctt
     3841 gctggtaaat tacgtttggc aaggaaagcg tggggtttgc taatccacaa ctattgctaa
     3901 cctgaagggt ttggttaaaa ttaccttcaa acgcagttcc acaatcttca aatagtgtga
     3961 gagtaacagc aaactggttt ggtccgatac attcgtaggt gatgttacca ccgggaacgt
     4021 gagatgcttt actggtgttg gaaatcaggg agatcccggt aacaacagta acaaatagca
     4081 ggcgattgat taaatgtttc atcctattaa cttagcttta ctaaaacatg acagatatca
     4141 ggcaaaaagg ttgcctaatt ggccgaaaca aatttacctt gcctttgcct gaaaatcaga
     4201 tgaaaagaag gagaattcaa gtatttaaca tttcgcattt tatttgttaa aatgaaggat
     4261 agcaataata gagaaatcac aataggatta actgggaata ttggggcagg aaagactaca
     4321 atagcggctg tatttcagga gtttggtata ccggtatttg atgcagacaa ggttgggaga
     4381 cggcttttgg ataccgatca ggatatcaag aatggactaa ttaaaatctt tgggaaggac
     4441 atccttacta acaacctgcc tgatcgaaaa aaaatagcaa atcttgtttt ttccgacgat
     4501 agcctgctga tcgaactgaa taagttgatt catccccggg tgggggaggt gttccgggaa
     4561 tggcgcaaaa atcagatggc aaaggttgtg attcgggaag ctgcaatatt gattgaaagc
     4621 gggactttta gggatctgga tatattgatt gttgttacat gtccggagaa tatccggttg
     4681 aaacgagtaa tggaacgtga tcattcgaat gaatcggaag ttcgggcacg catgaataag
     4741 caaatgaatg aagaagaaaa agttgctctt gcagattttg tcatcataaa tgatggtaaa
     4801 acgcttgtca ttccacaggt gctggatgtt ttgcaaaaat tgaaaatcaa caattaaaag
     4861 atactcatga ttattcccga tccggatcaa ctaaaacttc ttgataaacg cacgattgaa
     4921 attcagggta ttcgttcact ggatctaatg gagagggcag cattaaaatg gttggatgaa
     4981 tttctcaacg atctaaatct tatttctcga gtggggaaag gttccggtga tcagaccatt
     5041 catatttttt gcggtccggg aaacaatgga ggggatggtt tggttgtggc aagacatctg
     5101 atgattcagg gattttctcc tatagtgtat gttttgaata ttggtaaaaa gccaactgaa
     5161 gatcacctag aaaacaggaa aaaggcagaa caaaaaggga ttacgataat tgatttgaaa
     5221 gagtctgcac aattgccgga atttcgtgaa aatgattgtg taattgatgc actttttgga
     5281 tttggttttg taggcaaact ggaaggtttt gcggctgaat tggtggagca catcaacact
     5341 tccaatacca gaatttattc aattgatatt cccagtggtt tgccttcgaa tgaaaatccg
     5401 gaaggaaaaa caatagtgcg agcaagtgtc acattcactt ttcattcgcc aaaactatct
     5461 ttctttttcc cggagaatgc acaatataca ggacgatgga aagtggttga catcggattg
     5521 gaatggagtg agttaaaagc tgatcggaat gctgttcatt ttattcttcc tgaatctctt
     5581 gctgtatttc taaaaccaag agcaaaattt tcacataaag gcgattatgg tcatgctctg
     5641 ttacttgccg gaggaagggg taaagtggga gcagcacaat tatcaggatg tgcagcacta
     5701 aaaagtggat gtggattatt gaccattgca actcctttat gtggggaaat acccttgcaa
     5761 actgcattac cggaagcgat ggtcattgca gatgcggaaa acgattttat cagcatgctt
     5821 ccacatttgg ataaatatga tgcaattggt gccggtcccg gaattggttt ggataaacag
     5881 accggcaatg tgatcaaatt gttgatccag caaacacagg tgcccttggt tcttgatgcg
     5941 gatgcattaa atattcttgc tgagaataaa acctggatgt cgtttttacc agccggaaca
     6001 atcctaactc ctcatccggg tgaatttgat cgcttgtttg ggagacacac atccggttac
     6061 gacaggtaca ttaccttaaa ggaaaaagca caaaagcaca atgttatcat cgtgttgaaa
     6121 ggcgcatata cctgcatcgc acatcctgat ggcagtacct ggttcaactc aacaggaaat
     6181 cccggtatgg caaaaggcgg ttcaggtgat gttttaacag gagtgatact gggcttgctc
     6241 gccagaggat actcaccatt tcaggcaaca atgattggag tatggattca tggaaaggca
     6301 ggggatttat gtctgattac gtttggacaa gaatcagttt tggcatcaaa tatttgcgaa
     6361 aaaattgtgg atgcgttctc gtctttagaa agaaggcact attattaata gtgtttttta
     6421 tctctttttt tttaatgttt aagattgatt gtttttttag tatctttcta tttggcattt
     6481 tcgggtaatt agttaaatca gtaaacctat tattatggat atatcaaagg tcagaatatt
     6541 ggtttcagct attcagaaat tgtcacttgc aagagatttg gataggataa tggatatcgt
     6601 acggagatct gccagacaat tagttggcgc tgatggggcg acatttgtat tgaaagaggg
     6661 agagaaatgc tattatgctg atgaggatgc gataagtcct ctttggaagg gacagaaatt
     6721 tccgatttct gcttgtatta gtggttggtg tatgattaac aagaaatttg tagttattga
     6781 agatatttac aatgatgaga gggttcctat tgaagcatat cgcccaactt ttgtaaaaag
     6841 tcttgcaatg gtaccgataa gaacaattaa tcctgttggt gcaattggag tatattgggc
     6901 aaaattacat tcaccaacag atgaagatgt tgaagttttg caatcgctag ctgatattac
     6961 ctcagtatca attgaaaacg tttttgtata tgaggagtta aaggagcaga ataaaatttt
     7021 atatgatatt gcatttttgc aatctcatca ggttcgtgta cctgtagctc aattaaaagg
     7081 attatttgag ttgattaatt ttgatgattt gtcatttgaa aaaaatagtg agatttttga
     7141 acgaattaaa atcacagttg actcattcga taagacaata ttggaaataa cagaaatgtc
     7201 cagcaaaatt aaatattatc agtaaataaa tttttccgcc tgatt
//
LOCUS       DAOMYG010000117         7155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3518_length_7155_cov_11.598028, whole genome shotgun sequence.
ACCESSION   DAOMYG010000117 DAOMYG010000000
VERSION     DAOMYG010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3518_length_7155_cov_11.598028"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /locus_tag="PK610_05150"
     CDS             <1..1437
                     /locus_tag="PK610_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35350.1"
                     /translation="SRNDTREIAKRLKGETLFDSCKNIWQFVYGHIAYEKDEKGKEQI
                     RRPSRSWADRVRGVDCDDYTVFISSVLTHLGIPHSLRIAKYWWSEDKFAHIYPVVHTE
                     DGKQITVDCVVNRFNYEEPYTEKRDRKMDLYYLNGIERPTTVDAVDLTDGGLSELGKV
                     KILQKIGQGVKKVVHAVNKVNPATALLRAGVLASMKLNVFKVAENVRFAYLSDAEAQK
                     RKMNMAKFAKLKQVKDKLEKIFYGAGGQPENLKEAILTGKGNASKEVSGLMGNETQMP
                     LRDILGQRIFTSEIESLDGLGEPATGAALAAASAIMASLAAIIKSIGGLKGEDSGATP
                     TDASANANITDPNLTLPTENVNLDEFTIPTEEVTDDPGTNLQKRSTTTVTETGDGTNT
                     NKNADGTDPKTGWWAENKKWALPTGIAVGVAGLSFAAYKIAQNKKKEGKKGKKGGEGL
                     SGIHHGKKRKKKHYKKSKKKKAHAVRWK"
     gene            1467..2090
                     /locus_tag="PK610_05155"
     CDS             1467..2090
                     /locus_tag="PK610_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35351.1"
                     /translation="MKDEKNKYEKKKDRKSLKERMKNPSPHLKEAGQGLLRDLVVGVV
                     AGGATGAIIGRPSLVVGLVVNAAGHFMEMPGISSFGMGMMAANGFQGDGVTLSGEETA
                     MERAKQRLLNYKNNFTQKIYLDKIMKKADEKKTTESDKPVGEVKYFVYPNTDNADMSG
                     MDMTALDNIENQIIQSAENYQQKTPVPKVEKEVSGNDETLMMRDPLY"
     gene            2276..3004
                     /locus_tag="PK610_05160"
     CDS             2276..3004
                     /locus_tag="PK610_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35352.1"
                     /translation="MLGQLELIAESEYNNAGDLLGIDPMNVNGELLGALKAMNPIARQ
                     KVINAIAKNPAPSKGSRAEMEKHFRELQPHIKEELTKGNLRLADTLIYSIKAVNSKTV
                     KIFETQTDKEIGLTNISNAKLPKNMSMLVSGIILLVGTAAGATKDDSMATNFGKIEAF
                     PAIANGEFSLKANKKTIVPEIGNYVFKTENNHLLTLGYFKLANPRLIQDDVQIEMTIE
                     LGTVTGIPANTYVYAALHGTATIP"
     gene            3144..3428
                     /locus_tag="PK610_05165"
     CDS             3144..3428
                     /locus_tag="PK610_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35353.1"
                     /translation="MTRKERNFYNTNERKITALKKKGNIITFKERWAYGNGNAIQIIV
                     TGFKKEKSGAVKIEGRAYDSLWYKSMDELLNAVDWNFMNRNYEPDKRDFL"
     gene            3425..3814
                     /locus_tag="PK610_05170"
     CDS             3425..3814
                     /locus_tag="PK610_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35354.1"
                     /translation="MKETKNDKQIKRFDFSPANGSNQSYEFEVDKTAEFVTAVVLTSD
                     RDDRLFYRGSCKVEIGGIEYLPENFEAKLLMCGINVAPNSRVYQLGNVPVGNGKVKIT
                     YKDTNHPDAPFVAYRVSLYVHTLLKEC"
     gene            3808..4344
                     /locus_tag="PK610_05175"
     CDS             3808..4344
                     /locus_tag="PK610_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35355.1"
                     /translation="MLKDKVHIHSVNVTQVAETISFRLPLPRDLKRITGLFLTENTIL
                     RGVGDSVGKFSKADFLYATLKVGDLTLKERGKSDMFFAGEVIRNDSSMLYGDFVELDF
                     YNRPIPISPADFYKHEWFKAGTKWEEHILDLVPCSDYLIGFYRDRFNEFFAVGSNYRV
                     NIYLLYESEIKQKVCARN"
     gene            4329..4703
                     /locus_tag="PK610_05180"
     CDS             4329..4703
                     /locus_tag="PK610_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35356.1"
                     /translation="MRTELALKHSENRMKELGWKSVEMNLRHVRLLPSSKTTFAIGSD
                     MWILMEEQDLRNASGTYQFPASPIRIESANGVFDVLDNKVHEQEHEHSGKMEITNKVN
                     RLLYVMFLQITPKRKCSDGKKK"
     gene            4687..5478
                     /locus_tag="PK610_05185"
     CDS             4687..5478
                     /locus_tag="PK610_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35357.1"
                     /translation="MEKRNERFQDNTLEKIKRILEVWDEKGTPRFYSVQVDGETIVSR
                     TNNPGEFDHVKAFVFHNTEQIRIAYYRGNEIEPVEEVTYTVQKEKEKSGGLSGVEIDE
                     RISQALEAERRKWEHEQLKKELSETKKELTEAEDHIDNLETLVEELKSSKNKLGNVHV
                     GEVVSVMVESFLKRNVDKIKRLPGGSTLAGILADNEGGENNEPQKETEATYEKVEDEQ
                     DGWWNDVQKAFNQQELEYVKAIVTILAHEKNKIQDVFSIIQPGQK"
     gene            5492..5674
                     /locus_tag="PK610_05190"
     CDS             5492..5674
                     /locus_tag="PK610_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35358.1"
                     /translation="MQKFTYKITLEAENEKQAQEKMAALTVMARKLTAREIKKISEVV
                     EENGSTLKTAKNFLGL"
     gene            5674..6429
                     /locus_tag="PK610_05195"
     CDS             5674..6429
                     /locus_tag="PK610_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35359.1"
                     /translation="MSKQTAIGNKGQQNHPTPEAARGQNEYYPQQMQGEEKKDSWKQT
                     VLYGALAVGVTGLTIWAVRKMVQEAVSTREETKTLEDGSSATIAKQIKMAFDNDGWWG
                     TDEEALRRALRSTPSKDDFDKVKKSYQKLYHRSLLEDMADELTSTEYNEMLQIISAKP
                     KKKGDKVEPNYDAWAKRLRSAMELYYMGMFPGTDEEAIYAVFNEMPGATAWSKVKSAY
                     TRIYGDDLYQDLIGEMWSSEIDDLIAKVKRKGS"
     gene            6433..>7155
                     /locus_tag="PK610_05200"
     CDS             6433..>7155
                     /locus_tag="PK610_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35360.1"
                     /translation="MEKEKNKEQQTKKSNAKKIAIIGLGIAAVGAGTYFGWQYFKSQK
                     NKNVALDDDEETVPYTPSVQTTPVQTSYTPAYVPSTTTSSFPLKKGSRGEKVRQLQNA
                     LISRYGASVLPKYGADGDFGSEMATALANNGWPATIDETTFNVITAGSSSSSTSSFYP
                     MTTATNLYLAVVLRNFDSVLKELKKFRNVEDYKSANEYFKKKEIGGVSWTIVNALLTK
                     FTDSSQKDQFRKEFLRMGLKFDG"
BASE COUNT         2532 a         1334 c         1743 g         1546 t
ORIGIN      
        1 agccggaatg ataccaggga gattgcaaaa agattaaaag gagaaacgct ctttgattcg
       61 tgcaaaaaca tttggcaatt cgtgtacggg cacatcgcgt atgaaaaaga tgagaaggga
      121 aaggaacaga tacgcagacc gtcacgaagc tgggcggata gggtacgcgg agtggattgt
      181 gatgattaca cggttttcat ctccagcgta ctcacccacc tgggcatacc acacagttta
      241 cgcatagcga aatactggtg gagtgaagac aagttcgcac atatctatcc ggtggttcac
      301 acggaagatg gaaaacaaat cacagttgac tgtgtagtga accgatttaa ttacgaggaa
      361 ccatacacag aaaaaagaga tagaaaaatg gatttgtatt acttaaacgg aatagagcga
      421 ccgacaacag ttgatgcggt tgatctaacg gacggaggat tgtcggagtt gggaaaagtg
      481 aaaatcctgc aaaagatcgg tcagggagta aaaaaggtgg ttcatgcggt gaacaaagta
      541 aatccggcaa cggcattact cagagccgga gtgttggctt cgatgaaact caatgttttt
      601 aaagtagcgg aaaatgttcg ctttgcttac ctgagtgatg cagaagcgca gaaacgcaaa
      661 atgaacatgg cgaagtttgc caagctgaaa caggtaaaag ataaactgga aaaaatcttt
      721 tacggagcag gtggacaacc ggagaatttg aaggaagcaa ttttaactgg caagggcaac
      781 gcatcaaaag aggtcagcgg attaatgggt aacgaaactc aaatgccact gagggatata
      841 ttggggcaac gcatattcac aagtgaaatt gaatccctcg atggacttgg agaacctgct
      901 accggtgcag cccttgccgc agcttccgca atcatggctt cacttgcagc gattatcaaa
      961 agcattggag gacttaaagg cgaagattca ggagcaacac cgacagatgc cagcgcaaac
     1021 gcgaacatca cagatccaaa tctcacccta ccaacggaaa atgtaaacct cgatgagttt
     1081 actatcccta cggaagaagt tactgatgat ccagggacga atttacaaaa gcgaagtacg
     1141 accaccgtta ctgaaacagg agacgggacg aacaccaaca aaaatgctga tggtactgat
     1201 ccgaaaacgg gttggtgggc tgaaaacaag aaatgggctt taccaacagg aattgcggtc
     1261 ggggtggcag gattgagctt tgcagcctac aaaatcgcac aaaacaaaaa gaaagaggga
     1321 aaaaagggta aaaaaggagg tgaaggtcta tcaggaatcc atcacggcaa gaagcgcaag
     1381 aaaaaacact acaaaaaaag taaaaagaaa aaagcacatg ccgtgcggtg gaagtgaaat
     1441 aatgttgaat taaaaacacg caagtgatga aagacgaaaa aaataagtac gaaaagaaaa
     1501 aggatcgcaa gtcgctcaag gagcgaatga agaatccaag cccgcacctc aaagaagcgg
     1561 ggcaaggtct gctcagagac ctggtggtgg gagtggttgc gggaggtgca acgggggcga
     1621 taattggaag accttcattg gtggttggat tagtggtgaa tgccgcaggt cactttatgg
     1681 aaatgccggg aatatccagt ttcggtatgg gaatgatggc tgccaacgga ttccagggag
     1741 atggagtaac actcagcggt gaagaaacag caatggagag agcgaagcaa cgactactca
     1801 attacaaaaa caatttcacc caaaagattt acctggataa aatcatgaag aaagcggatg
     1861 agaaaaaaac aaccgaaagt gacaagccgg ttggtgaagt aaaatacttc gtctatccca
     1921 ataccgataa tgctgacatg agcggtatgg atatgaccgc tttggacaat atcgaaaatc
     1981 agatcataca atcagcggaa aactatcagc agaaaacgcc tgttccaaaa gtggaaaagg
     2041 aagtaagcgg aaatgatgaa acgctgatga tgcgcgatcc cctctactaa cgggtaaagg
     2101 gctaccgcag ggaagagttc cttaatcaaa agacgagaag taaaaaaatc tgccagagag
     2161 cggacaggga gattcttgcg cctgaaaaaa cggaaagtac ttgggtagcc caaccgtagg
     2221 gggcaaaaaa agaaaagtga agaaggaaaa aaagtttaac cattaaaaca aaaaaatgtt
     2281 aggacaactg gaactgattg cagagagcga atacaacaac gctggagatc tgctcggaat
     2341 tgacccgatg aatgtcaacg gagagttgct tggcgcactc aaagcaatga atcctattgc
     2401 acgtcaaaaa gtaattaatg ccattgcaaa aaatcctgca ccaagcaaag gatcacgcgc
     2461 agagatggaa aaacatttcc gcgaattgca accgcacatc aaggaagaat tgacaaaagg
     2521 aaacctgcgc cttgcagata ctttgatcta ctcgatcaaa gcggtgaatt caaaaacggt
     2581 gaaaattttt gaaacacaaa ccgacaaaga aattggattg accaatatca gcaacgcaaa
     2641 attgccgaaa aatatgtcaa tgcttgtgag tggaatcatt ctcttggtgg gaacagcagc
     2701 aggtgcaacc aaagacgatt cgatggcgac caattttgga aaaattgaag ccttccctgc
     2761 cattgcaaac ggagagtttt ctcttaaagc aaataagaaa accattgtac cggaaatcgg
     2821 aaactatgtt ttcaaaaccg aaaacaatca cctgttaaca cttgggtatt tcaaattggc
     2881 aaatccgcgc ctgatccagg acgatgtgca gattgaaatg accatcgaat tgggaactgt
     2941 gacaggcatt ccggcaaaca catacgtgta tgcggcattg cacggaacgg caactattcc
     3001 ataaccaaaa atctttcatg ttcacacaag cccgaaggga aaaagtgatg cggagtttaa
     3061 actgccattt ctccgcacac ttttcctgaa gggttatgaa agaaaatcaa aaaatgaaat
     3121 gggggtaaag tgatttgaaa acaatgacaa ggaaggaacg gaatttctat aacaccaatg
     3181 aaaggaaaat cactgctctt aaaaagaaag ggaacatcat cactttcaaa gagcggtggg
     3241 cttacggaaa cggaaatgcc attcagatca ttgttacagg attcaaaaag gaaaaatccg
     3301 gtgctgtaaa aatcgaagga cgtgcctacg attccctatg gtacaaaagc atggatgaac
     3361 tactgaatgc tgtcgattgg aattttatga atagaaatta cgaaccggac aaaagagatt
     3421 ttttatgaaa gagacaaaaa acgacaaaca gatcaaacga tttgattttt ctccggctaa
     3481 cgggtcaaat cagtcctacg aatttgaggt agataaaacc gcagaatttg taacagcggt
     3541 ggtattgact tctgatcgtg atgatcgttt gttctatcgt ggttcgtgca aggttgagat
     3601 tggtggtatt gaatacttac ccgaaaattt tgaagccaag ttgctaatgt gcggaatcaa
     3661 tgtagcaccc aatagcagag tgtatcaact cggaaatgtt ccggttggaa atggcaaagt
     3721 gaaaatcact tacaaggaca cgaaccatcc tgatgcgcct tttgtggcat accgtgtttc
     3781 gctttatgta catacgcttt taaaagaatg ctaaaagaca aagtacatat ccattctgta
     3841 aatgttaccc aggtagcgga aacaatcagc tttcgtttac cccttccaag ggatttaaaa
     3901 cgaataacag gattgttcct tacggagaat actatactcc gtggagtagg tgattcggtt
     3961 ggtaaatttt ccaaagcaga ctttctctat gccacgctca aggttggtga tttgactttg
     4021 aaggaacgtg gaaaatcgga tatgttcttt gcaggtgaag tgattcgtaa tgatagttca
     4081 atgctctatg gagattttgt tgaactggat ttttacaacc gaccgattcc aatttctccc
     4141 gcagactttt acaaacacga atggttcaaa gcaggaacga aatgggaaga acacattttg
     4201 gatttggttc catgttctga ttacctgatc ggattttacc gcgaccgctt caacgaattt
     4261 ttcgctgtgg gaagcaatta cagggtgaat atttatttgc tctatgaatc ggaaataaaa
     4321 caaaaagtat gcgcacggaa ttagcactca aacatagtga aaaccgaatg aaagagcttg
     4381 gttggaaaag cgtagagatg aacctgcggc atgttcgttt gcttccttcg agtaaaacca
     4441 ctttcgctat tggcagcgat atgtggattc tgatggaaga acaggacttg agaaacgcaa
     4501 gcggaactta tcagtttccg gcaagtccga ttcggattga atctgccaac ggagtatttg
     4561 atgtactcga caacaaagtg catgagcagg aacacgaaca cagcggaaaa atggagatca
     4621 ccaataaagt taacagactt ctgtacgtga tgtttttaca gatcacacca aaaagaaaat
     4681 gcagcgatgg aaaaaagaaa tgaacgattt caggacaaca cccttgaaaa aataaaacgc
     4741 atccttgaag tatgggatga aaagggaaca ccgcgatttt attcggtgca ggtagatggc
     4801 gagacaattg tatcgcgcac taataaccct ggagagtttg accatgtgaa agcatttgtg
     4861 tttcacaata ccgaacaaat ccgcattgct tattacagag gaaatgagat tgagcctgtg
     4921 gaagaagtaa cctatacggt tcaaaaagaa aaggaaaaat cgggtggact tagcggtgta
     4981 gagattgacg aacgcatttc tcaggcgttg gaagcggagc gcagaaaatg ggaacacgaa
     5041 caacttaaaa aagagttaag cgaaaccaaa aaggaactca cagaagcgga agaccacatt
     5101 gacaaccttg aaacattggt ggaagaacta aaaagttcca aaaacaaatt aggcaatgtg
     5161 catgtgggtg aagtggtgag cgtgatggta gagagttttc tcaagcgaaa cgtggacaaa
     5221 ataaaaagac ttccgggagg ctctaccctt gcaggtattc ttgccgacaa tgagggaggc
     5281 gaaaacaatg aaccacagaa ggaaaccgaa gccacctatg agaaagtgga agacgagcaa
     5341 gatggttggt ggaacgatgt gcaaaaggca ttcaatcagc aggaacttga atatgtgaaa
     5401 gccattgtta ccattctcgc tcatgagaaa aacaaaattc aggatgtttt ttcaatcatt
     5461 cagcctggac aaaagtaagg aggaaaaata aatgcaaaag ttcacttata aaattacgct
     5521 tgaagcggaa aacgaaaaac aggcacagga aaaaatggct gccctaacgg taatggcaag
     5581 gaagctaact gccagggaaa ttaagaagat ttccgaagta gtagaagaaa acggaagcac
     5641 attgaaaact gcgaaaaact ttttaggact gtaatgagca aacaaactgc catagggaat
     5701 aaggggcaac agaaccatcc gacaccggaa gctgcacggg gacaaaacga atactaccca
     5761 cagcaaatgc agggtgaaga aaaaaaagat tcgtggaagc aaacagtgtt gtatggtgcg
     5821 ttggcagttg gtgtaacagg gcttacgatt tgggcggtgc gaaagatggt acaggaagca
     5881 gtcagtaccc gtgaagaaac gaaaacgctc gaagacggaa gctctgccac cattgccaag
     5941 cagattaaaa tggcgtttga caatgacggt tggtggggaa cggatgaaga agcattgaga
     6001 agggcactca gatcgactcc aagtaaagat gattttgata aggtgaaaaa atcctatcag
     6061 aaactttatc accgcagttt gcttgaggac atggctgatg aattgacctc gacagaatac
     6121 aatgaaatgc ttcagatcat ttctgccaaa cctaaaaaga aaggcgacaa ggtagaaccc
     6181 aattacgatg cctgggcaaa gcgactcaga tcggcaatgg aactctacta catgggaatg
     6241 ttcccaggaa ccgatgagga agccatttac gcagtgttta acgaaatgcc tggtgcgaca
     6301 gcctggtcga aagtgaaaag tgcctatacc cggatttacg gtgatgatct gtaccaggac
     6361 ttgattggag aaatgtggtc atccgaaatt gatgatttga ttgcaaaagt gaaacgaaaa
     6421 ggaagctaag tgatggagaa ggaaaaaaat aaagaacagc aaacaaaaaa gagcaacgca
     6481 aaaaaaattg cgatcattgg tttgggtata gctgccgttg gcgcaggtac atatttcgga
     6541 tggcaatatt tcaaaagtca aaagaataaa aacgttgcgc tcgatgatga cgaggaaaca
     6601 gttccttata caccatcggt tcaaactact ccggttcaga cttcctacac acctgcgtat
     6661 gttccttcga caacaacaag tagtttccct ctgaaaaaag gaagtcgcgg agaaaaagta
     6721 cgtcaactgc aaaacgcact catctcacgt tacggagcat cggtgctacc gaaatacgga
     6781 gcggacggag atttcggaag tgaaatggca actgcacttg ccaataacgg atggcctgcc
     6841 accatagatg aaaccacttt taatgtgatt actgccggat catcttcatc atccaccagt
     6901 tcgttttatc caatgacaac agccacgaac ttgtatttag cagttgtgct gagaaatttc
     6961 gatagcgtat tgaaggaact gaaaaaattc cgcaacgtgg aagactacaa aagtgccaat
     7021 gagtatttca agaaaaaaga aatcggtggc gtgagttgga caatcgttaa tgcactcctt
     7081 accaaattca cagacagttc acagaaagat cagttccgca aagagttttt acgaatggga
     7141 ttgaaattcg atggc
//
LOCUS       DAOMYG010000118         7150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3523_length_7150_cov_7.628612, whole genome shotgun sequence.
ACCESSION   DAOMYG010000118 DAOMYG010000000
VERSION     DAOMYG010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3523_length_7150_cov_7.628612"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PK610_05205"
     CDS             complement(<1..634)
                     /locus_tag="PK610_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HRJ35361.1"
                     /translation="MKKRALILGFLIAQLPVWAALNPVVDSIPMRDGKKLAADVYIPI
                     GCTSCPTILIQTPYNRLLYRFGLPLGVGQQIDNSPYVYVIVDWRGFYGSSSAMIASPN
                     RGQDGYDVVDWISQQSWSDGKVGTWGPSALGKIQYETAREQHPAHLCAVPLVASPRFE
                     YEEYYHGGVYRTEYLQQLDALGYGLSSVVLANPVKNLTWTFAYNQSNYPAS"
     gene            802..2313
                     /locus_tag="PK610_05210"
     CDS             802..2313
                     /locus_tag="PK610_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35362.1"
                     /translation="MIKSLTKSEKRFFKLSSSLQEGEKNYLKIFDSIDKQQEYDEESI
                     KNQFNGETFIRHFPSEKNHLYKLILKSLRSYHADNSISSILRQEIKNIEILFKKGLYK
                     ECHKFLQRSKKLAIEHEKFYYQFELINIEKQLLEEDYESGEFDKDLNALVEEETQCIE
                     KLRNLAEYHIIFSRINFVFRREGFAHNEEERAIVQEIEDYHLIKGKNTALSARASTIC
                     YYIKGLCATTNRQYQDAFTFFLKTKEIFDKNPHIRTDLAKRYLKTLTSLLYGYIDTNN
                     IEAANNLIKVLVDLKEEDGFDSLEMEVKIFTSTHIAQLMLLNKTGDFRKAIELSKDVN
                     SAMDKYGERINKEQRLLFTYTISYAYFGAGDYKEALQWINDVLNMNEQTLRRDVFNFA
                     RVFNLLIHLELGNYDLLEYTVKSTVRYLNKKEKDYQSETVFIKHLRKLIRSNSDMERK
                     EIYEEMYQEFRELLNNPIEQVILEYVDILSWLESKRKNISFAEAVRARSLTNA"
     gene            complement(2296..2754)
                     /locus_tag="PK610_05215"
     CDS             complement(2296..2754)
                     /locus_tag="PK610_05215"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HRJ35363.1"
                     /translation="MKRYLFAILFLAACSSEHELGRLEGSWREDSGTETFFLEDWTMN
                     ADNSLSGLGRMCSGKDTLSMERLRIEKKNDTWHYRVFLQTESGEVNTDFKLMNSIQGD
                     SLVFENKENDFPAQIIYVFLGKDELTIYLNPASFNSTDKPIVLHMLRIGK"
     gene            complement(2751..3515)
                     /gene="rlmB"
                     /locus_tag="PK610_05220"
     CDS             complement(2751..3515)
                     /gene="rlmB"
                     /locus_tag="PK610_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRJ35364.1"
                     /translation="MEYNDKDNPDLLFGTRAVIEAVRSGREINKIMVQRGLSNELFNE
                     LKNELRGSEIQIQFVPSEKLNRLTRKNHQGVIGFVSPVSYSRIEDLLPIVFEEGRVPL
                     FVILDRITDVRNFGAICRTAECMGADAVIIPSRGGAQITSDAVKTSAGALNRLPVCKE
                     DNLKKTIHYLAASGIKVVSCTEKAELELSGSDLTVPLCIIMGSEEDGISGEYLKLSDE
                     RVKIPMAGSIASLNVSVAAGMMLYEVTRQRISVLQQ"
     gene            complement(3515..4771)
                     /locus_tag="PK610_05225"
     CDS             complement(3515..4771)
                     /locus_tag="PK610_05225"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HRJ35365.1"
                     /translation="MNSIRFFWVVCFACVFSAQASGQRVIRTDQQRTKADPVKPDVVV
                     YHSSLDYSEVHFRISSRDIMYARTESGEFTAHLVVNYRLFTLESVKQVVDSGTLTIND
                     TKSDVWEKFIGGSFKVKMPQGSTYSLKIQFVDKKKNSSMELNITADKKEYFNRQNFLL
                     RSSEVVNCSPYLQQRNVSVESPFHKGKQFWVRVYRRSFSLAPPPFSEQRLQQFKYEAD
                     SIYQVRMDEQGKFNLILPEGGFVHIVPDTSSRVGLSLFHFPKYFPRVMTPDALIEPLR
                     YICSSEEFKTLRTAIDQKKSIDQFWLDRSSSRERARELIRNYYNRVQESNRRYSSYLE
                     GWKTDRGMIYLIFGEPAFIDRTTDSETWIYGDRFSTASLRFNFLKIQNPFSDNDFLLQ
                     RMPVYKPEWYKAVDVWRSGRVYYYLN"
     gene            complement(4810..6897)
                     /locus_tag="PK610_05230"
     CDS             complement(4810..6897)
                     /locus_tag="PK610_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ35366.1"
                     /translation="MKKLLTLVLTLSLFSALAQQKDMPFEKDLFKDRKDEFKTAKKAF
                     EAGEEFFKQYQFDKAEVEFDKAYAFNPNYSKLNYYLGMCVLNSSYKFKALNYFKKAYE
                     LNPNVAPEINLLIGIGYHINGDWDNGIKHYEYHKKVLNPKNTDTLEYINKKISECKTG
                     KDLVAKPKRVWVDNLGKNVNTEFPEYGPFISADESVIIYTGRRSDTRGGKKDEGDGKF
                     FEDIYLSKRNPKNGEWEKAINIGENINTENHDAPSGLSPDGKLLYIFYGWKGGGDIYQ
                     SKLKDGQYGKPEKLSSNVSSKYYESSASVSFDGKELYFASERPGGFGGEDIYVSRWDE
                     KKKEWGPAENLGANINTKYRETGVFLHPDGETMYFSSQGHSTMGGFDIFMSKRDEKGV
                     WGAPVNIGYPINSPDDDVFFVVSGSGRYGYYSSFRQDGYGEKDLYRITFLGPEKPPIP
                     STEDNLIASLVNPVREIVIEPKVEVSTVNLAILKGIIRDARTLQPLEAKLELIDNDKN
                     ELVTTLTSDSKSGAYLVTIPAGKNYGISVKAEGYLFHSENVDIPKASGYKEYEKNVDL
                     KKVEVGQTIVLRNIFFDYDKATLRDASRNELERLIKLLNENPTLRIEISGHTDTQGDA
                     TYNQKLSENRAKAVVEYLVRAGISASRLEYKGYGESKTQISEADILKMKTKTEREDAH
                     QQNRRTEFKILSK"
     gene            complement(6915..>7150)
                     /locus_tag="PK610_05235"
     CDS             complement(6915..>7150)
                     /locus_tag="PK610_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35367.1"
                     /translation="IKMQKYKEAVADCDKAIQLDAAYGVAYLNRGIAKEMLRDLAGAC
                     SDWENAAAMGVESGETYYNIYGVCSEVEKYNQK"
BASE COUNT         2188 a         1555 c         1273 g         2134 t
ORIGIN      
        1 ttgaagctgg atagttggat tgattgtatg cgaatgtcca tgttaggttt ttgacaggat
       61 tcgcaagtac aactgaagat aaaccataac ccagggcatc caactgctgt aaatattcag
      121 ttcgataaac acctccgtga taatactcct catattcaaa tcggggactt gcaacaagag
      181 gcacagcaca taaatgagct ggatgttgtt cgcgagcggt ttcatactgt attttaccaa
      241 gtgccgatgg cccccatgtt cctacctttc catcactcca tgattgttga ctaatccaat
      301 ccacaacatc ataaccgtcc tgcccccggt ttggtgaagc tatcattgca gaagaggacc
      361 cataaaatcc cctccaatcc acaattacat acacataagg agaattatcg atttgctgac
      421 caacacccaa aggcagtcca aaacgataca aaagtctgtt ataaggcgtt tgaataagaa
      481 tggttggaca agacgtgcag ccgatgggga tgtaaacatc ggcagcaagt tttttaccat
      541 cacgcattgg tatagaatca actacgggat tcaatgcagc ccagacaggc aattgagcaa
      601 taaggaatcc aagaataagt gctctttttt tcataactat caaatatact tcgcttatca
      661 ttgaaataaa agaagtcgaa tggggaaact ttgagtgctt gattccttta tcttgatttc
      721 ttatctttaa acacagaaaa acaccttctt acaggcttta attgcaggaa tcctatgaaa
      781 cccagcacgg aactcttcga attgataaag tctcttacga aatcggagaa acgcttcttc
      841 aaactcagct cttcgcttca ggaaggagaa aagaattatc tcaagatttt cgactccatc
      901 gacaaacaac aggaatatga tgaagagtcc atcaaaaatc aattcaacgg ggaaacgttt
      961 atacggcatt tcccatctga aaagaatcat ttgtacaaac tgattctgaa aagtctgcgt
     1021 tcgtaccatg ctgataattc cattagttca attttaaggc aggaaataaa aaacattgaa
     1081 atactcttta agaaaggcct gtacaaggaa tgccataaat ttttacagcg ctcaaaaaaa
     1141 cttgcaatcg aacatgaaaa attctattat cagtttgaac tcatcaatat tgagaaacaa
     1201 ctcctcgagg aggattatga atccggtgag tttgataagg atttaaatgc tttggtagaa
     1261 gaagaaacgc aatgcattga aaaacttcgc aatctggcag agtaccatat tattttttca
     1321 aggatcaact ttgtattccg tcgtgaaggc tttgcgcaca atgaagaaga gcgggcgatt
     1381 gtgcaagaaa tcgaagacta tcacctcatc aaaggaaaaa acacagcact gtcagcacgg
     1441 gcctcaacca tttgctatta catcaaagga ttatgtgcta ctacaaaccg tcagtatcag
     1501 gatgcattca ccttcttttt gaagaccaaa gaaatttttg acaaaaaccc acacatcaga
     1561 accgatctgg caaaacgcta tttaaaaact cttaccagtt tactttacgg ttatattgac
     1621 accaacaata ttgaagcagc aaacaacttg atcaaagtac ttgttgacct taaagaagag
     1681 gatgggtttg actcccttga gatggaagta aaaatattta cttccaccca tattgcccag
     1741 cttatgcttc ttaataaaac cggtgacttc cgtaaggcga ttgaactttc caaggacgta
     1801 aattctgcaa tggataaata cggtgagcgt attaacaaag agcaacgact tctatttacc
     1861 tataccattt cctacgcata ttttggagca ggtgattaca aggaagcctt gcaatggatc
     1921 aatgatgtac tcaacatgaa tgaacaaaca ttacgtcgtg atgtgttcaa tttcgcccgt
     1981 gtatttaatc ttctcattca cctggaactt ggcaactacg atttgctgga atacactgta
     2041 aaatcgaccg tgcgatatct gaataaaaaa gaaaaagatt atcagtctga aactgtattt
     2101 atcaaacatt taagaaaact aatcagaagc aattctgata tggagcgaaa agagatttac
     2161 gaagagatgt accaggaatt cagagaactt ttaaacaacc ctatagagca ggtaattctg
     2221 gaatatgttg atattctttc gtggttagaa agcaaacgca aaaatatcag ctttgcggaa
     2281 gcagtacgcg cacgatcact taccaatgcg taacatatgc aacacaattg gcttatcagt
     2341 ggaattaaat gaagcaggat tgaggtagat tgttaactca tctttcccca gaaatacata
     2401 aataatttgt gcaggaaagt cattctcttt gttctcaaat accaatgagt ctccctgtat
     2461 tgaattcatc aatttaaaat cggtatttac ttcgccgctt tcggtttgaa gaaacaccct
     2521 gtaatgccat gtatcatttt tcttttcaat cctcagccgc tccatcgaga gcgtatcttt
     2581 tccggaacac attcgtccta gtccgctcag cgaattgtca gcattcatcg tccaatcttc
     2641 cagaaaaaac gtttccgttc ctgagtcttc cctccacgaa ccttctagcc ggcctaattc
     2701 atgctcggaa gaacatgctg ctaaaaagag tattgcaaag aggtagcgtt tcattgctgc
     2761 aatacagaaa ttctctgccg tgtcacttcg taaagcatca tccctgcggc aaccgataca
     2821 ttaagggaag cgatagatcc tgccattggg attttcaccc gttcatcact cagttttaaa
     2881 tattctcctg aaataccatc ttcttctgaa cccataataa tacaaagcgg aacagttaaa
     2941 tcagatcccg ataattccag ctctgctttt tcggtacacg ataccacctt gattccgctt
     3001 gcagccaaat aatgaatggt ttttttcaaa ttatcttctt tgcaaacagg aagccgattc
     3061 aatgcaccgg cagatgtttt tactgcatca gaagtgatct gagcacctcc acgcgaggga
     3121 ataattacag catccgcacc catacattct gcagtacggc agatggcacc aaaatttctt
     3181 acatcagtta ttcgatcgag gattacaaaa agcggtaccc tgccttcttc aaatacaata
     3241 ggtaagagat cctcaattcg ggaataagaa acaggactaa caaaaccaat tacaccctgg
     3301 tgattctttc gggtaagacg atttagtttc tcagagggta caaactgaat ttgaatctca
     3361 gatccccgta attcattttt gagttcatta aataactcat tactcagacc acgctgaacc
     3421 atgattttgt tgatctctct gcctgaccga acagcttcaa taactgcacg tgttccgaat
     3481 aacaaatcgg gattatcttt atcgttgtac tccatcaatt caaataataa tataccctac
     3541 cacttctcca aacatcaacc gccttgtacc actccggttt ataaaccggc atgcgttgca
     3601 atagaaaatc attatcggaa aaggggtttt gaattttcaa aaaattaaag cgcaaacttg
     3661 ccgtgctgaa acgatcacca taaatccatg tttcactatc tgttgtacga tcgataaaag
     3721 ccggttcgcc aaaaatcaga taaatcatcc ctctgtcggt tttccaacct tcaaggtaac
     3781 ttgaatacct gcgatttgat tcctgcactc tgttgtaata attgcggatc aattctcggg
     3841 ctctttcacg actggaagac cgatccaacc aaaactgatc gatggatttt ttctgatcga
     3901 tggctgttct caacgtttta aattcctcag aactacagat ataccggagg ggttcgatca
     3961 aagcatcggg agtcattacg cgtgggaaat actttgggaa atggaataaa gacaatccaa
     4021 cacgcgatga agtatcggga acaatgtgta caaatcctcc ttcaggtaaa atcaggttaa
     4081 attttccctg ctcatccatc cgaacctggt aaatactgtc ggcttcatat ttaaactgtt
     4141 gcaagcgctg ttctgaaaat ggtggaggcg ctagtgaaaa tgatcgtctg taaacccgga
     4201 cccaaaactg ttttccctta tgaaatggtg attctacact cacattccgc tgctgcaagt
     4261 aaggtgagca attcactact tccgatgatc gtaataagaa attctgccga ttaaaatact
     4321 ctttcttatc tgcagtaata ttcaattcca ttgaagaatt ttttttctta tcaacaaatt
     4381 gaatcttcaa agaataggtt gatccttgtg gcattttaac tttaaaactg ccgccaataa
     4441 atttttccca aacatcgctt ttggtgtcgt taattgttag cgtaccacta tcaacaacct
     4501 gttttacact ttcgagcgtg aacaagcggt aattcacaac caaatgagct gtgaattcgc
     4561 ctgactctgt acgtgcatac ataatatctc ttgaggaaat ccgaaaatga acttcagaat
     4621 aatccaatga ggaatggtaa acaacaacat cgggtttgac gggatcagct tttgttcttt
     4681 gctgatcggt acggataacc ctttgtcctg aagcctgcgc tgaaaacaca caagcaaaac
     4741 atacaaccca aaaaaagcgt attgaattca tattataaat taagcggtcc cgattgggac
     4801 cgctcgattt tattttgaaa ggattttaaa ttctgttcga cggttctgct ggtgggcatc
     4861 ttcgcgttct gtttttgttt tcattttaag aatatcagct tcggaaatct gtgttttcga
     4921 ttcgccatag cctttgtatt ccaatctgct tgcacttatt ccggcacgaa caagatactc
     4981 aacaacagcc ttcgctctgt tttcagaaag cttttggtta taggttgcat caccttgtgt
     5041 atcggtatgc ccgctgattt caatacgaag agttgggttt tcgtttaaga gtttgattaa
     5101 acgctccagc tcgtttctgg atgcatcacg aagtgttgct ttatcataat cgaagaaaat
     5161 attacgcaat acaatcgttt gtccaacttc tactttcttc agatccacat tcttctcgta
     5221 ctctttgtaa cccgaagctt ttggaatgtc aacgttttcg gagtggaaaa gataaccttc
     5281 agctttaaca gaaataccat aattttttcc tgctggtatg gtaaccaaat aagcacctga
     5341 tttggagtca ctggttaaag ttgttacaag ctcgttctta tcattatcga tcaactcaag
     5401 ttttgcttcg agtggctgaa gtgttcttgc atcacgtatt atccctttaa gtatggcaag
     5461 atttacagtg ctaacctcaa ctttgggttc aataacgatt tcgcgaactg gattaacaag
     5521 tgaagcgata aggttatcct ccgtggaagg aattggtggt ttttccggtc caaggaatgt
     5581 aatacgatac aaatcctttt cgccataacc atcctgacgg aaagaggaat aataaccgta
     5641 gcgaccacta cctgaaacca caaagaacac atcatcatca ggagaattga tcggatatcc
     5701 aatgtttacc ggagctcccc aaacaccttt ctcatcacgt ttgctcatga aaatatcaaa
     5761 gccacccata gtactatgac cttgtgaaga aaaatacatt gtttcaccat cagggtgtag
     5821 gaatacacct gtttctctgt atttggtatt aatgttggca cctagattct cagccggacc
     5881 ccattctttt ttcttttcat cccaacgaga aacataaatg tcttcaccgc caaatccacc
     5941 aggacgttcc gaagcgaaat acaattcttt accatcgaaa gaaacagatg cagaggactc
     6001 gtaatacttc gagcttacat tgcttgatag cttttcgggt ttaccgtatt gaccatcctt
     6061 caacttggac tgataaatat ctcctccgcc tttccagcca taaaaaatat aaagcaattt
     6121 accatcgggc gaaagtccgg aaggcgcatc gtgattttcg gtattgatat tctcaccaat
     6181 attaattgcc ttctcccatt ctccgttttt cggatttctt ttcgataaat agatgtcctc
     6241 gaagaattta ccatctcctt catccttctt tcctcctcgg gtatcactgc gacgaccggt
     6301 gtaaataata actgattcgt cagcagaaat aaatggtcca tattccggaa actccgtgtt
     6361 cacattctta cccagattat ctacccaaac acgttttggt tttgcaacca aatctttccc
     6421 ggttttgcat tcactaattt tcttattgat gtattcaagg gtatctgtgt tttttggatt
     6481 gagtactttt ttatgatact cataatgctt aatcccgtta tcccaatcac cattgatatg
     6541 ataaccaatt ccaataagca aattgatttc aggcgcaaca tttgggttga gctcgtaagc
     6601 ttttttgaaa tagttcaagg ctttgaactt ataagaagag ttcaaaacac acattccgag
     6661 gtaataattc aacttcgaat aattcggatt aaaggcatag gccttatcaa attccacttc
     6721 cgctttatcg aattgatact gcttaaaaaa ttcctctccg gcttcaaaag ctttttttgc
     6781 ggttttaaat tcatctttgc ggtccttgaa aagatctttt tcaaatggca tatccttttg
     6841 ttgagccaat gcgctaaaca atgaaagcgt aagaaccagt gtgagcagct ttttcatata
     6901 aattcctcct gtaatcattt ctggttatat ttttcaactt ccgaacaaac tccataaatg
     6961 ttgtaatatg tttctccgga ttcaacaccc attgcagctg cattctccca atcactgcaa
     7021 gctccggcca aatcgcgtaa catttctttt gcaatgccac gattcaagta tgcaactcca
     7081 taagcagcat ccaactgaat agctttgtcg caatcggcaa ctgcttcttt gtatttttgc
     7141 atttttatgt
//
LOCUS       DAOMYG010000119         7149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3524_length_7149_cov_9.900902, whole genome shotgun sequence.
ACCESSION   DAOMYG010000119 DAOMYG010000000
VERSION     DAOMYG010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7149
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3524_length_7149_cov_9.900902"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2465
                     /gene="hrpB"
                     /locus_tag="PK610_05240"
     CDS             <1..2465
                     /gene="hrpB"
                     /locus_tag="PK610_05240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HRJ35368.1"
                     /translation="ILPELKKKLSDNILLLLQAPPGAGKSTWLPLELLQEPWLNNKKI
                     LLLEPRRLAARTVAQRLAHNLGEDTGEIIGYRIRFETKISHKTRIEVVTEGILTRMLQ
                     NDNALEEYGLLLFDEFHERNLNADLALALSREIQQVLRPDLRILIMSATLGCESLASE
                     LGNVPVVISEGRQFPIEYRYHEPNTQLQLAENCVALIQKASAETEGDILAFLPGSGDI
                     LRAAELLEKNNSSLSVATLYGDMPFSEQQHALLPDSNGKRKVILSTSIAETSLTIEGI
                     RVVIDSGFTRSPRFDTRSGFTKLETVKISQDTATQRAGRAGRTSSGICYRMWAKHTHQ
                     QLTEHRKPEILDADLAQLTLELAGWGQENIESLKWVTAPPIANVAQAKTILKNLNAID
                     DKGKITPEGKKILEIPSHPRIAHLLLTGKKQSCPALACDVAALLEERDPLRRSESADL
                     LLRIEALHKWRKKERTDADRHSLERIERVSLQWRKLLKTETIDEHINPFAVGSLIAAA
                     FPERIARKTEQEGRYRLGNGRMARLPKNDSLSDADWIAVATLDAGSGEGKIFLAAPFD
                     HNDLLKIATEHETIRWDNREELFIARKEWKVGDLIVKEVPLNTITSDQRSNAICEALK
                     RSGKEMLDWSDELLQLQNRVLSLRKWNPEMMLPDLDTDVLIQNASHWLAPYLSEARTG
                     NDLKKINLHNAIMHSIDWNVQQEIDSLAPKKIKVPSGSEIALEYFEDGRSPILAVRLQ
                     ECFGLTETPTVNQGKTKVLMHLLSPGYKPVQITQDLPSFWNNLYHEVRKELKIRYPRH
                     SWPEDPWKAEAVRGAVKRRSEK"
     gene            complement(2536..3474)
                     /locus_tag="PK610_05245"
     CDS             complement(2536..3474)
                     /locus_tag="PK610_05245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HRJ35369.1"
                     /translation="MRTRFSSLLVLVLFSCSLEAKLNELKEFGNNPGNLRAFLHSPIN
                     FSDTNSAPLVVVLHGCNQDAHAIFDEAGWGMLSDKMGFYVLCPEQKLLNNGMRCFNWF
                     LGADNSANQGELGSILQMINYTISLKGIDKKRIFIYGVSAGGYMSVACMANYPGVFKS
                     GAILAGGPYFGPGTVPLGKKLSANQLAQRVFDLFPGAAPTFPSMLILHGTKDPVVDYS
                     NDELLFDQWSLLHGLPLPASSDTIVKKENTFYRKQNFNKDSLLLVELLALKGLGHYLS
                     VDPGNGVEQGGKMGLFAKDKNWFSTLYVVKFFGLLH"
     gene            complement(3474..3869)
                     /locus_tag="PK610_05250"
     CDS             complement(3474..3869)
                     /locus_tag="PK610_05250"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRJ35370.1"
                     /translation="MPDFKKYYEIPASPEDVYNALTNPFMIEIWTGEKAEMSTEPGSE
                     FSLWDGSIVGKNLEFEEGKRVVQQWYFGEQEEPSIVNIKLHEHKKGTSLELKHTNIPD
                     DDFNDIVEGWNESYMAALIEFFEHSESPE"
     gene            complement(3898..4458)
                     /locus_tag="PK610_05255"
     CDS             complement(3898..4458)
                     /locus_tag="PK610_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35371.1"
                     /translation="MSVLLSLTEIASQCPLYLNYAKGIEEGSFLFECADLILNRSQLI
                     CFRNKFRITEAELFVFQEGHWDPFTLGAEGKKAGDMQSKPGQWFLHGSGIEYTFGEPG
                     KFRAILLRGIEPVEGKGEKTENAWDTREQLLRMCNDDLIRNLRMEQASFAKRDIQFSA
                     RLNLQRKIDPKFADLPYRFSAIPLVE"
     gene            4636..>7149
                     /locus_tag="PK610_05260"
     CDS             4636..>7149
                     /locus_tag="PK610_05260"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRJ35372.1"
                     /translation="MQIRVFSQEPFAYTLNDENGLPSNEVYRVTQDNEGYIWIGCDAG
                     LFRYDGLNFIPFSNPKQNSRGISGLVFDHENRLWCHNFSGQLFRVEKNKLELICDFSN
                     EANKYQITHGDKRGFWKISSSAITLYDFNGKLISSYAQPLLNDAFSYGLAVKYFNDKL
                     WLDLPNRGLHFFRESTGELISVAEPFVINQDCQARDFFVKDSMLYLLRSETYPSTWMH
                     LYEIDAKKINMRRIIDLHNLVQIRNYFFYEDRLHQLWIGSSFGAICYPDVRAFTSPRM
                     VIYTNSRVSSVLHDKEGNYWFSTLQRGILMAPDLDVIYMDTLNSPLKNNAICHLKMLS
                     NDLLAIGNFNGQFQLYYEDWFLPKVILPESVNAFIGITVKDIEEYGENILISRGRLFI
                     YNKINGKAFYDKDFSNIRDMCIWQDTLYIITPISVNKASLNELEEKKGLDFITIKNSG
                     GWKVEVDRSSGDVYYAMNDGLFVYSQGKLTKILNENESINVSCIAERKGSIWVGTQSM
                     GLLEIKNKKIVRSRLKPESSVDYNSIKLVYADSIDVWIITDQKIYRLDPEKDHLEIVS
                     KNIGISSKEITSVTGNKSYLYLGTAKYMQRISRKISAVNSTPPDVKLTLITCENDTIK
                     SQKNGIRLNYDFTNLQFHFIALSYGSWNKLRYEYRMQGLDTSWNTNLFNNTTAIYSSL
                     PPGEYTFQVRALNESGISGNYIEVPIVVSPPLWQKGWFYLIVSIVAIVLVVFFFQYRI
                     RLIRKRNTLEKKVIASQLTALKAQMNPHFMYNALNSIQDLMLSQDLQNSNRYLGRFSS
                     LMRKVLDASGKESITLEEECAMLELYLDLEKLRFGDGFSF"
BASE COUNT         2214 a         1539 c         1432 g         1964 t
ORIGIN      
        1 agatactgcc cgaattaaag aaaaaacttt cggacaatat tctgcttttg cttcaggctc
       61 ctccgggtgc aggaaaaagt acatggcttc ctctcgaatt gttacaggaa ccctggttaa
      121 acaataagaa aattctctta ctcgaaccac gcaggctcgc agctcgcaca gttgcgcaac
      181 gattggctca taatcttggt gaagataccg gagagattat tggttaccgt atacgctttg
      241 aaacgaaaat aagtcataag acccggattg aagttgttac agaaggaatt cttacccgaa
      301 tgttgcaaaa cgataatgca ctggaagaat atgggcttct attgtttgat gaatttcacg
      361 agcgcaatct gaacgccgat cttgcacttg cactcagcag ggaaatacaa caggtattac
      421 gtcctgatct ccgcatcctc attatgagtg caacactggg ctgtgagtcc ctggcaagtg
      481 aactcggtaa tgttccggta gtgattagtg aaggacgaca attcccaata gaataccgtt
      541 accacgaacc caatacccaa cttcagcttg cagagaattg cgtggcattg atccaaaaag
      601 ccagcgcaga aacagaaggg gacatccttg cctttttacc gggatcagga gacatattgc
      661 gcgctgcaga acttttggaa aaaaataatt cttcgctatc cgttgcaacg ctatatggag
      721 atatgccttt ttcggaacag caacatgcat tgctacccga ttcaaacggg aaacgaaaag
      781 ttattctctc cacctccatt gcagagacca gccttaccat tgaaggaatt cgcgttgtta
      841 ttgattcagg ttttacccgg agtccacgtt tcgacacccg ctcgggattc actaaactcg
      901 aaacggtgaa gatcagtcag gacaccgcca cacaacgcgc aggacgcgca ggaagaactt
      961 catcaggaat ttgttaccgc atgtgggcaa agcacaccca tcagcagttg acagaacacc
     1021 gcaaacccga aatacttgat gcagatcttg ctcaattgac acttgaatta gcgggctggg
     1081 gacaggaaaa tattgagtcg ctaaagtggg ttaccgctcc gccaatagca aatgttgcac
     1141 aggcaaaaac cattcttaaa aacctgaatg caattgatga caagggaaaa attactccgg
     1201 aaggaaaaaa aatactggag atccccagtc atccccgtat tgcccattta ttgctgacag
     1261 gaaagaaaca atcgtgcccg gcacttgcat gcgatgtagc cgccttattg gaagaaagag
     1321 atcctttacg aagaagtgaa agtgcagatc tgttactccg cattgaagcg ctccacaaat
     1381 ggcgaaaaaa agaacgaacg gatgccgacc gtcattcatt agagcgcatc gaacgcgttt
     1441 cgctacagtg gagaaaacta ctgaaaaccg aaacaattga tgaacacata aatccctttg
     1501 ctgttggttc cttaattgca gcagcatttc cggagaggat tgcacgaaaa acggaacaag
     1561 aagggcgtta tcgactagga aacggaagaa tggctcgtct cccgaaaaat gatagtctaa
     1621 gtgatgccga ttggatcgcc gttgcaacac tcgatgccgg aagtggcgaa ggaaaaatat
     1681 ttcttgccgc accatttgat cataatgatc tgctcaaaat tgccactgaa catgaaacca
     1741 tccgatggga taacagagaa gaacttttta ttgcgcgcaa agaatggaaa gtcggcgatc
     1801 tgatcgtaaa agaggttcct cttaacacca ttacttccga tcaacgcagt aatgccattt
     1861 gcgaagcact caaaagatca ggaaaagaaa tgttggattg gagcgatgag ctgctgcaat
     1921 tacaaaaccg tgtgttatcc ctgcgcaaat ggaatcctga aatgatgcta ccggacctag
     1981 acacggacgt tttgattcaa aatgcttcac actggctggc gccctattta tcggaggcaa
     2041 gaaccggcaa tgacctgaag aaaataaatc tgcacaatgc gattatgcat agtatcgatt
     2101 ggaatgtgca acaggaaatc gattcgctcg ctccaaaaaa aataaaggtt ccgagcggat
     2161 cagaaattgc attggagtat tttgaagatg gtcgttctcc tatccttgct gttcgactcc
     2221 aggaatgttt tgggttgact gaaacaccaa ctgtaaatca aggaaagaca aaagtattaa
     2281 tgcatcttct gtcgccggga tataaacccg ttcaaatcac acaggacctt ccaagttttt
     2341 ggaacaatct ctaccatgaa gtcagaaaag aattaaaaat ccgctatccc cgtcactcct
     2401 ggccggaaga cccctggaaa gcggaggcag ttagaggagc tgtaaagcga agaagcgaga
     2461 aataggatgt tggaaacgac aaaaaattaa aagggatgaa ttgctttccc atagaattgc
     2521 aaaaatccta attacctagt gcaataatcc aaaaaacttt accacataca aagtgctgaa
     2581 ccaattcttg tcttttgcaa ataagcccat ctttcctcct tgctcaacac catttccagg
     2641 atctacagaa aggtaatggc ctaatccttt tagcgccagc aattcgacaa gcaaaaggct
     2701 atccttgtta aagttttgct ttctgtaaaa ggtattttcc ttttttacga tggtgtcaga
     2761 agatgccgga agtggtaagc catgcaagag gctccattga tcaaagagta attcatcatt
     2821 ggaataatca acaacgggat cttttgttcc atgcagtatc agcatcgacg gaaatgtagg
     2881 tgcggcaccg ggaaacaaat caaaaacacg ttgcgccaat tgattcgcag aaagtttttt
     2941 cccaagtggt actgtccccg gaccaaaata gggcccaccg gctagaattg ctccggattt
     3001 gaatactccc ggataattgg ccatacaggc aacgctcata tatccaccgg cagaaacccc
     3061 gtaaataaaa attcttttct tatctattcc ttttaaacta attgtatagt tgatcatttg
     3121 caaaattgat ccaagctctc cttggtttgc tgaattatca gcgccaagaa accaattgaa
     3181 acatcgcatt ccgttattga gtaatttttg ctccgggcat aatacataga accccatttt
     3241 atcggatagc atcccccatc ctgcctcatc aaaaattgca tgtgcatcct gattgcaccc
     3301 atgtaaaaca acaaccaatg gagcgctgtt ggtgtcagaa aaatttattg gggaatgaag
     3361 aaaggcgcgt aaatttccgg ggttgtttcc aaactccttt agttcgttta gtttggcctc
     3421 cagagaacat gaaaacaaaa ccaatactaa caaactagaa aaacgagtcc gcattattcg
     3481 ggactttccg aatgctcaaa aaattctatc agtgctgcca tatagctttc attccaacct
     3541 tcaacaatat cgttgaaatc atcatcggga atattggtgt gtttcagttc cagtgatgta
     3601 ccctttttgt gttcatgcag cttgatgttc acaatagatg gttcttcctg ctcgccgaaa
     3661 taccattgct gtacaactct tttgccctcc tcaaactcca ggttttttcc cacaatactg
     3721 ccatcccata acgaaaactc acttcccggc tctgtcgaca tctctgcttt ctcgccggtc
     3781 cagatttcaa tcatgaaggg attggtcaat gcgttgtaaa catcttctgg agaagcaggt
     3841 atttcgtagt actttttaaa atcgggcata tctctaattt tatctgtaaa ctaattttta
     3901 ctctactaat ggtattgctg aaaaacgata aggtaaatcc gcaaacttgg gatcaatttt
     3961 tcgctgcaaa ttcaatcgcg cagaaaactg aatgtctcgt tttgcgaatg aagcttgctc
     4021 catccgcagg ttgcgtataa gatcatcatt acacatccgt agtaattgtt ctcgggtgtc
     4081 ccaggcgttt tctgtttttt ctcctttgcc ttccacaggc tcaatacctc tcaataaaat
     4141 cgctctgaat tttccgggtt ctccaaatgt gtattcaatc cccgatccat gaaggaacca
     4201 ttgtccgggt ttgctctgca tatcacctgc tttctttcct tcagcgccca gcgtaaaggg
     4261 gtcccaatga ccttcctgaa aaacaaataa ctctgcttct gtaatacgga atttgtttct
     4321 gaaacagatg agttgagaac ggttcagaat caaatctgcg cattcaaaga ggaaagaccc
     4381 ctcttcaatg ccctttgcat agttaaggta caaaggacac tggcttgcaa tttctgtaag
     4441 agataagagt accgacacct tgcaaagata ggaaatagga atgagaagaa acgagggata
     4501 ccccttgagt ttcattagaa aaacaatccc tcgtaaattt atggctttat tcagtatttt
     4561 cgtttcatgc ttattcggat gaactatgta accccaatta tattccggct ccttttcatt
     4621 caaatgctgt ttctgatgca aatacgggtc ttttcgcaag aaccttttgc ttacaccctg
     4681 aatgatgaaa acggattacc ctccaatgaa gtttaccggg taacgcagga caacgaaggt
     4741 tatatttgga ttggttgtga tgccggtttg tttcgctatg acgggctgaa ttttatccca
     4801 ttttccaatc caaaacaaaa ctcaagagga atttcaggtt tggtttttga ccacgaaaac
     4861 cgcctatggt gccacaattt cagcggacaa ttattcagag ttgaaaagaa caaactggaa
     4921 ttgatctgtg attttagcaa cgaagcaaac aaataccaga tcacccacgg agataaaaga
     4981 ggcttctgga aaatttcaag ctctgcaata acattatacg atttcaatgg aaaactaatc
     5041 tcctcttatg ctcaaccttt gttgaatgat gcgttctcct atgggcttgc tgtaaaatat
     5101 ttcaatgaca aactttggct tgacctcccc aaccgaggct tacatttttt cagagaaagc
     5161 accggagaac tcatttctgt tgctgaaccc tttgtaatca accaggattg tcaggcaagg
     5221 gatttttttg ttaaagattc tatgctttac cttttgcggt cggaaaccta cccaagtacc
     5281 tggatgcact tgtatgagat cgatgcaaaa aaaataaata tgcgtcgcat tattgacctc
     5341 cacaatctcg ttcagattcg aaactatttc ttttatgagg atcggctcca tcaattgtgg
     5401 ataggatctt cgttcggcgc aatttgttat cctgatgttc gcgcatttac atccccacgt
     5461 atggtgatct acaccaattc cagagtgtcg tctgttctgc atgataaaga aggaaactat
     5521 tggttcagca ccttacaacg tggaatatta atggcgcctg accttgacgt gatctatatg
     5581 gatacactga acagtccact taaaaacaat gctatttgtc accttaaaat gttatcgaac
     5641 gacctgctgg cgattgggaa ttttaatggt caatttcaat tgtattatga agactggttt
     5701 ttacccaaag taattttacc ggaaagcgta aatgcattta ttggaattac agtgaaagac
     5761 attgaggaat atggagaaaa tattctcatt tcaagaggtc ggttattcat atacaacaag
     5821 atcaatggaa aagcttttta cgataaggat ttctccaaca tacgtgatat gtgtatttgg
     5881 caggacacac tttatatcat tacacctatt tccgttaata aggcttcact taatgagctt
     5941 gaagagaaaa aaggactaga ctttataaca ataaaaaatt caggaggatg gaaagttgaa
     6001 gttgatcgct catcgggtga tgtttactat gctatgaacg atgggttgtt tgtttattca
     6061 cagggaaaac ttacaaaaat tctcaatgaa aatgaaagca taaacgtaag ttgcatcgca
     6121 gaaagaaagg gttcgatttg ggttggaaca cagtcgatgg gacttcttga aatcaagaat
     6181 aaaaagattg tccgctctcg gttaaaacct gaatcttctg tagactacaa ttcaataaaa
     6241 ttggtatatg ctgacagtat agacgtttgg ataattacag atcaaaaaat ttaccgcctt
     6301 gatccggaaa aagatcattt ggaaatcgta agcaaaaaca ttggaataag cagtaaagaa
     6361 attacatccg ttaccggtaa taaatcctat ctctatttag gaactgcaaa atacatgcaa
     6421 cgcatctccc gcaaaatttc tgcagttaac tcaacgccac ccgacgttaa acttacactc
     6481 atcacttgcg aaaacgatac gataaagtcc caaaaaaacg ggatccgact taattatgat
     6541 tttaccaatc ttcaatttca ttttattgct ctctcttatg gaagttggaa taaactgcga
     6601 tacgaatacc gaatgcaagg gctagatacc agttggaaca ctaacttatt taataacaca
     6661 accgcaattt attcatcact tcctcctggg gaatatacat ttcaggttcg cgctttaaat
     6721 gaaagcggaa tttccggaaa ttatattgaa gtccccatag ttgtatctcc tccgctatgg
     6781 caaaaaggct ggttctacct cattgtttcc attgtcgcta ttgtattggt cgtgttcttc
     6841 tttcaatacc gcattcgact gattagaaaa agaaacacgc tggagaaaaa agtaatagca
     6901 tcccagttaa ccgcgcttaa agctcaaatg aatcctcact tcatgtataa cgcactcaac
     6961 tccattcagg atttgatgtt gtcgcaggat cttcaaaact caaaccgata cctgggaaga
     7021 ttcagttcac tcatgcgaaa agtactcgat gcatcaggaa aagaatcgat cactctcgaa
     7081 gaggaatgtg ctatgctcga gctgtacctc gatcttgaaa aactacggtt tggtgatggc
     7141 ttttctttc
//
LOCUS       DAOMYG010000120         7069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3584_length_7069_cov_7.780011, whole genome shotgun sequence.
ACCESSION   DAOMYG010000120 DAOMYG010000000
VERSION     DAOMYG010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3584_length_7069_cov_7.780011"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1519)
                     /locus_tag="PK610_05265"
     CDS             complement(23..1519)
                     /locus_tag="PK610_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRJ35373.1"
                     /translation="MAREFQIQVLPEDTINDAQLRKAIAKSIGVSETDLGPYRIDRRS
                     IDARGRQVKFTMKITVFDPNEKIPGETYVFEAKNVSQAEEVHIIGAGPAGLFAALRVI
                     ELGKKPVIFERGKTVRDRRRDLAILTKNHIVNPESNYCFGEGGAGTYSDGKLYTRSTK
                     RGDVKQILSLLVAHGADESILIDAHPHIGTNKLPGIIEAIRDSIRKCGGEVHFESKLT
                     DLSTENGNIRSITINGDQKIPVNNLILATGHSARDIFYLLHKKNILIEEKTFALGVRA
                     EHPQSIIDQAQYHCTVRGPYLPPASYSLVEQVKDTGVYSFCMCPGGIIAPCATSQEEI
                     VTNGWSPSRRNNPFANSGIVTTVTEEELKNFRQFGPLSALKFQEAIERACWIAGGKTQ
                     TAPAQRMIDFIEGKTSSTLPDCSYHPGIKSVSLKEVLPETLASRLKFGFKAFAQKMKG
                     YGTNEAVLVAPESRTSSPVRIPRNKTSLHHPQIKGLIPCAKNERLRNQ"
     gene            complement(1523..2254)
                     /locus_tag="PK610_05270"
     CDS             complement(1523..2254)
                     /locus_tag="PK610_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRJ35374.1"
                     /translation="MSHRIHTILALVLFLLGSPFLLAQKERVGNLKNFDKKPYHFGFI
                     LAYNTSNFYLDTYNAFPTSDSLMWIGAERQPGFDLGPLASLNLSKNISLRFIPAISFQ
                     DRRLRYGFYRTTNGELQNDEVIRRVESTYINLPFNLKLRTDRVNNFAAYAVVGYQYSI
                     DMASQKDVVNTFGREAIVRIRRPDHAWQVGGGLDFFLPYFKFGLELKVSSGFKNLLLQ
                     DNNMYTSPIDRLRSKVVLFSFTFEG"
     gene            complement(2303..3034)
                     /gene="ubiE"
                     /locus_tag="PK610_05275"
     CDS             complement(2303..3034)
                     /gene="ubiE"
                     /locus_tag="PK610_05275"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HRJ35375.1"
                     /translation="MGTPVKPYNQDDSKKQQVAQMFNNISHRYDFLNHFLSMGIDHIW
                     RRKAIGILRKESPKHILDIATGTGDFAIAALKLHPEKVTGIDISVGMLEKGREKMKKR
                     GYDNRVEMLEGDSENLPFADASFDAITVGFGVRNFENLEKGLGEMLRVLRPGKTAVIL
                     EFSKPRRFPVKQSFNFYSRRVIPFIGRTISKDKAAYTYLPESVEAFPEGKDFLRILEK
                     VGYTDVKAKTVSGGIATIYTGHKSR"
     gene            3357..3881
                     /locus_tag="PK610_05280"
     CDS             3357..3881
                     /locus_tag="PK610_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRJ35376.1"
                     /translation="MAGVLVSVDEPQKVEACFVLGGSSYERGLFAVKMFEKGYADKFI
                     CTGENVPSILEALGQAMTEAEVTAFYMRSAGIPDSSLAVINKSTSTREEAKVIREYCE
                     SNGLKKVMLLSSKFHTARMRKVFRSEFRSSSVEWIIVGAPSTKYNEFKWWESEEGMIA
                     LNNEWMKTIWYWFK"
     gene            3931..5544
                     /locus_tag="PK610_05285"
     CDS             3931..5544
                     /locus_tag="PK610_05285"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HRJ35377.1"
                     /translation="MDFFLNIKWMYFLGGLGLFLFGIRLLEDALVQLVGRSFKRFLKR
                     HTGTPVKAVLAGTITTAIIRSSSMVTLLVMSFTGAGIIGLKSGLGIILGANLGTTVTG
                     WIVSAVGFSFDLKALTLPFFALGGLMSMFSKSRLREGGHFLIGFSLMFLGLDFLKDAF
                     AELSGKFDLSGWEGSPKIFFFLAGFGLTAIIQSSSASVAIYLSMLASGVVSLEQAIFL
                     AIGSDLGTTVTALLGTINANAVRRKTGWGQFFMNVFQALLSIALFPLYVWFLQEVMGL
                     HNPLYLLVAFQSLANIVLIIVMIPLLGWFTRFIDKKIKTGEVHLTGFLHEVQASEPFS
                     ACVALQNEALKFGKETLRLNESIVAETFQGSVLFEKYDRLKSYENEINQFVVLMQQSQ
                     MPEQRSKQLTALSSSIRDFAFCAKEMKDIRHNLEDLLNSSDDEKFRMFHLIRSLQNDY
                     YKAVHAILKDPGTFQHTVNEYLSGMNRDITSKMQEVAMKLISNPKKHPSVNFSSLMNM
                     IREIESSNISMLKAVENYLVSVSLSNSES"
     gene            5574..6512
                     /locus_tag="PK610_05290"
     CDS             5574..6512
                     /locus_tag="PK610_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRJ35378.1"
                     /translation="MARKLSEIDLRKVGVYLVGNRSEYEHIFKDSLWILLGIACAAFG
                     LEGFIVPNGLLDGGVTGVSLIMAKLTPLNVPFYIFVLNLPFIFLGFRQVSKIFAVKTF
                     VAIIVLSAVLLVLQTMHVPRITDDKLLIAVFGGFFLGAGIGFAMRGGCVIDGTEVLAL
                     YMTRKLSMQVGEIILLINVLIFLIAASLFGIEMALYSILTYMAASKTVDYITNGIEEY
                     TGVIIISPEHEKIRKEIISQLGRGVTIYNGKRGLRGKKALESGLNDRDILFTVVTKLE
                     LTRLRNIVDEIDDSCFMTTHAMTGVKGGMIKQRKLH"
     gene            complement(6669..>7069)
                     /locus_tag="PK610_05295"
     CDS             complement(6669..>7069)
                     /locus_tag="PK610_05295"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HRJ35379.1"
                     /translation="TEIKWMTWTEAVAANKKKPKMIFVDVYTAWCGWCKVMDNKTFAV
                     EEVAKYMSEHFYCVKFDAEQKEDVTYRKQVYKYNSQYRSHELAVNFLDGQMSYPSFVI
                     FDKNEKRLEIIKGYIEVEPWMAKLKTIVGK"
BASE COUNT         1850 a         1463 c         1636 g         2120 t
ORIGIN      
        1 ttcaggagca accagcactg cttcattggt tccgtaacct ttcatttttt gcgcagggga
       61 taagaccttt gatttgaggg tgatgaagac ttgttttatt acgcggaatt cgaacgggtg
      121 atgaggtgcg gctttcagga gcaaccagca ctgcttcatt ggttccgtaa cctttcattt
      181 tttgcgcaaa tgccttgaaa ccaaatttta gtcgcgatgc cagcgtttcc ggcaatactt
      241 ctttcaagga aacacttttt attcccggat ggtaggaaca atcgggtagg gtggaagatg
      301 tttttccctc gatgaaatct atcatgcgtt gtgcaggtgc agtttgtgtt ttgcctccgg
      361 caatccaaca ggcacgttct atggcttcct gaaatttcaa tgcgcttaag ggaccaaact
      421 gacgaaagtt ttttaattct tcttcggtaa ctgtggttac aattccggaa ttggcaaatg
      481 gattatttct gcgtgatggc gaccaaccgt tggtaacaat ttcttcctgc gaagtggcgc
      541 aaggggcaat gattcctccc ggacacatac aaaacgaata gactccggta tcttttacct
      601 gctctaccag cgaataggat gcgggtggga gatatggtcc gcgaacggtg caatggtatt
      661 gtgcctgatc gatgatgctt tgcggatgtt ctgcacgcac accgagcgcg aatgtttttt
      721 cctcgatgag aatattcttt ttgtgcaaga ggtaaaaaat atcacgcgcc gaatgtccag
      781 ttgcaagtat cagattgttg accggaattt tttgatcacc attgattgta atggaacgaa
      841 tattcccgtt ttcggtgctg agatcggtga gtttggattc gaagtggact tcaccaccac
      901 atttgcggat gctgtcgcga atggcttcaa tgatgccggg taatttattg gttccgatat
      961 gaggatgtgc atcaatcaga atagattcat ctgctccgtg tgcaaccaat agcgagagaa
     1021 tttgttttac atcaccgcgt tttgtagaac gggtatataa ttttccatcc gaataggttc
     1081 ctgctccgcc ttctccgaaa cagtaattgg attcaggatt tacaatgtgg ttttttgtaa
     1141 ggatggcaag atctcttctc ctgtcgcgaa cagtttttcc gcgttcaaaa atgaccggtt
     1201 ttttacctaa ctcaatcact cgcaaggccg caaataatcc tgcgggaccg gcaccgataa
     1261 tgtggacttc ttctgcctga cttacatttt tggcttcgaa aacatacgtt tctcccggta
     1321 ttttttcgtt gggatcaaaa acggtgattt tcattgtgaa ttttacctgt cgtccccgtg
     1381 catcgataga tcggcggtcg atgcggtagg gccccagatc cgtttcgcta acaccaatgg
     1441 atttggcaat ggctttgcga agttgtgcat cgttgatggt gtcttcgggc aggacctgaa
     1501 tctgaaactc gcgggccacg atttatcctt cgaaggtgaa tgaaaacaac accactttag
     1561 aacgtaaacg atcaatgggt gaagtgtaca tgttgttgtc ctgaagcaac agatttttaa
     1621 atccggaaga aacttttaac tccagtccaa acttaaaata aggcaggaaa aaatccagtc
     1681 caccaccaac ctgccaggca tgatccggtc ggcgtatgcg cacaatggct tctctgccga
     1741 aggtgttgac gacatctttt tgcgatgcca tatcaatgct gtattggtaa ccgaccaccg
     1801 cataagctgc aaaattgttt acccggtcgg tacggagttt gaggttgaat ggaagattga
     1861 tataggtgga ttctacgcgt cgtattactt cgtcgttttg caattctccg ttggtagttc
     1921 tgtaaaatcc gtaacgcaaa cgacggtcct gaaacgaaat tgcaggaata aatcgcaagg
     1981 aaatattttt actcagattg agcgaggcca gcggaccaag atcaaagcca ggttgtcttt
     2041 ctgcaccaat ccacatcagc gaatcggatg taggaaaagc attgtaggta tcgaggtaaa
     2101 aattggatgt attgtaagca agaatgaatc cgaagtgata cggtttcttg tcgaaattct
     2161 tcaggttacc cacacgctct ttctgggcaa gcagaaatgg acttcccagg agaaacaata
     2221 caagcgcaag aatggtgtga attcgatgtg acattacctc caaaaataat cattaaaacg
     2281 gcagtagttc cgttttgttg ccttaacggc ttttgtgacc ggtataaatc gttgcaattc
     2341 ctccgctgac ggttttggct ttaacatcgg tataccccac tttttcgagg atgcggagaa
     2401 aatccttccc ttccggaaag gcctccaccg actcaggtaa ataggtgtat gctgctttat
     2461 cttttgagat ggttctacca atgaagggaa tcactcgcct cgagtagaaa ttgaacgatt
     2521 gcttaaccgg aaatcgacgt ggtttgctga attccagaat gacagcagtt ttgccgggac
     2581 gaagaacacg aagcatttct cccagcccct tttccagatt ttcgaagttt cgaactccga
     2641 atccaacggt aatggcatcg aaagaagcat ctgcaaaagg aaggttttcg gaatcgcctt
     2701 ccagcatttc aacacgatta tcgtaaccgc gctttttcat tttttcgcga cccttttcca
     2761 gcattccaac agaaatatcg atgccggtaa ctttttcagg atggagcttg agtgctgcaa
     2821 ttgcaaaatc gcctgtgcct gtggcaatat ccagaatatg tttggggctt tccttgcgca
     2881 aaattccaat ggcctttctt cgccaaatat ggtcgatacc catactcagg aaatgattca
     2941 gaaaatcata acggtgcgaa atgttattga acatctgtgc gacctgctgt tttttgctgt
     3001 cgtcctgatt ataaggcttt acgggtgttc ccatgggtgg aattgaggtg caaaggtagt
     3061 gtttttcgac ttaaggaaat aacacgaatc ggacccaatt gtttcgaaac aggctaaatc
     3121 aaactttgtg cgctctaatt attcgtttcc tatcttcgtt gtgccgaacg ttaggcagta
     3181 ttttttgatg ctgctgaaaa tgaaatccga tggagaaaaa acgttgagct tacatgtaga
     3241 tggaaataaa aacacgactc aaaaattttt ttcacaagcg acggatcatt cggtggtcag
     3301 tattttttgc aattctgcta ttgctttttg ttttccgctt ttcaataatg cgtggaatgg
     3361 caggcgtact cgtttcggtt gatgagccgc agaaagtgga agcctgtttt gtattggggg
     3421 gaagctccta cgaacgcgga ttatttgctg tgaagatgtt cgaaaaggga tatgccgata
     3481 aatttatctg taccggtgag aatgttccat ccattctcga agcactcgga caagccatga
     3541 cagaggctga agtcacggcg ttttatatgc gctctgcagg aatacccgat tcttcgctgg
     3601 cggtgatcaa caaatcgact tccacccgcg aagaggcaaa ggtgattcgt gaatactgtg
     3661 agtcgaacgg tttgaaaaaa gtaatgttgc tctcctccaa atttcacacc gcacgcatgc
     3721 ggaaagtttt tcgctcggaa ttccgctcct cttcggtcga atggatcatt gtgggagctc
     3781 ccagtacgaa atacaatgaa ttcaaatggt gggagtctga agaaggaatg attgcgctga
     3841 ataacgaatg gatgaaaacg atctggtatt ggtttaaatg aggtgtgtca tattcgcata
     3901 acgatcagct ggtaaatttg caatcataga atggattttt tcctgaacat aaagtggatg
     3961 tattttttgg gtggactcgg actttttttg ttcggtatcc gtctgctcga agatgcactt
     4021 gttcagttag tgggacgttc attcaaacgt ttcctgaaac gacacacggg gactcctgta
     4081 aaagcggttt tggcaggaac aattaccacg gcgattattc gcagcagttc aatggtcacc
     4141 ctcttggtga tgtcgtttac cggagctgga atcatcggat taaaaagcgg tttgggaatc
     4201 attttaggcg ctaatctggg tacaacggta accggatgga tcgtttctgc ggttggattt
     4261 agttttgatc taaaagcgct tactcttccc ttttttgcat tgggaggatt gatgtccatg
     4321 ttcagcaaga gccggctccg agaaggtggt cattttctga tcggattcag cctgatgttt
     4381 cttggactgg attttttgaa agatgcattt gcggaattat caggcaagtt tgatctcagt
     4441 ggctgggaag gtagtcccaa aatatttttc tttttagcag ggtttggact taccgctatt
     4501 attcagtcga gctcagcatc tgttgcgatc tatctttcga tgcttgccag tggtgttgta
     4561 tcactcgagc aggctatttt tcttgctatc ggttccgatc tgggaactac ggttaccgct
     4621 ttattgggaa ccatcaatgc aaatgcagta cgtagaaaaa caggatgggg acagttcttt
     4681 atgaatgtat ttcaggccct gctttccatt gcattgtttc cgttgtatgt ctggttttta
     4741 caagaagtaa tgggattaca taatcccctc tatttgctgg ttgcttttca gtcgcttgca
     4801 aatattgttt tgataattgt tatgattccg ctgctgggat ggtttacgcg tttcattgat
     4861 aaaaagatca aaacgggtga agtacaccta accggatttt tgcatgaagt acaggcatcc
     4921 gaaccttttt ctgcatgtgt cgccctgcag aacgaagcgt taaaatttgg aaaagaaacc
     4981 ttgcgtctga acgaaagtat tgttgcagaa acgtttcagg ggagtgtgct ttttgaaaag
     5041 tacgatcgat taaaatccta tgagaacgag atcaatcaat ttgtggtgct catgcagcag
     5101 tcgcaaatgc cggagcagcg ttcaaaacaa cttaccgcgc tgagttcatc cataagagat
     5161 tttgcttttt gtgccaagga gatgaaagat atccggcata acctcgaaga ccttttgaac
     5221 agttcggacg atgagaaatt cagaatgttt catttgattc gttccctgca aaacgactat
     5281 tacaaagcgg ttcatgccat actaaaagat ccgggcacct ttcaacatac agtgaacgaa
     5341 tacctcagtg ggatgaacag ggacatcacc tcaaaaatgc aggaagtggc aatgaagctg
     5401 atcagtaatc ccaaaaaaca tccttctgtg aatttttctt ccctcatgaa tatgatccgg
     5461 gaaatcgaaa gttccaatat ttccatgctg aaagcagttg aaaactacct ggtttccgtt
     5521 tctctttcga acagtgaaag ctgattggtt tttttttctg acattgctgc gaaatggcac
     5581 gtaaactatc agaaatcgac ctgcgaaagg ttggtgttta ccttgttgga aaccgctcgg
     5641 agtatgagca tattttcaaa gattcgcttt ggattctcct gggtattgca tgtgcagcat
     5701 tcggactcga aggttttatt gttcccaatg gtctgctcga cggcggtgta acaggtgtgt
     5761 cgctgatcat ggccaagctt accccgctaa atgttccctt ttacatcttt gtactcaatc
     5821 ttccttttat tttccttggc tttcggcagg tatccaaaat atttgcggtg aaaacctttg
     5881 tggcaattat tgtgctttct gcagtgttgc tcgttctgca aaccatgcat gttccgcgga
     5941 ttaccgacga taaattactc attgcagtgt tcgggggatt ttttctcggt gcaggtatcg
     6001 gctttgcaat gcgcggcgga tgtgttattg atggaacaga agtccttgcc ttgtacatga
     6061 cccgcaaact gagcatgcag gttggggaga tcattcttct aatcaatgtt ttgattttcc
     6121 tcatagcagc aagtctcttc ggtattgaaa tggctttgta ttcgatcctg acttacatgg
     6181 cagcttccaa aacggtcgat tatatcacaa acgggataga agaatacacc ggtgtaatca
     6241 tcatctctcc cgaacacgaa aagatccgca aagaaattat ttcccaattg ggacgcggag
     6301 ttaccattta caatggaaaa cgcggattgc gcggaaaaaa agcattggaa agcggattaa
     6361 acgaccgtga tattctcttt actgtcgtca ccaaactgga gctgacccgc cttaggaata
     6421 ttgttgatga aattgacgat tcctgtttca tgacaacgca tgccatgacg ggggtaaaag
     6481 gaggaatgat caaacagcgg aaattacatt aagccggctt acctgaaata aaaggagctg
     6541 atataaacca aggtaaaaaa aatcagcgcg agtaaaacat aaacaatgat cctgttttcc
     6601 tcacgcggag gcagaaatcc gaaagtcttc agatcccacc agcgtttgtc gtgcggttgt
     6661 tccattgttt atttgcctac aatggttttc agttttgcca tccagggttc aacttcaatg
     6721 tatcctttaa taatctccag gcgcttttcg ttcttgtcga aaatcacgaa actgggatac
     6781 gacatttgtc cgtcgaggaa attcacagca agctcatgtg agcggtattg cgaattgtat
     6841 ttataaacct gcttgcggta ggtcacgtct tctttttgtt ctgcatcaaa ttttacacag
     6901 taaaaatgct cgctcatgta tttggcaact tcttctactg caaaggtttt attgtccatt
     6961 accttacacc atccgcacca ggctgtgtaa acatcaacaa aaatcatttt tggttttttc
     7021 ttgttggccg caacagcttc tgtccaggtc atccatttaa tttctgtgg
//
LOCUS       DAOMYG010000121         7059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3593_length_7059_cov_7.839806, whole genome shotgun sequence.
ACCESSION   DAOMYG010000121 DAOMYG010000000
VERSION     DAOMYG010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7059
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3593_length_7059_cov_7.839806"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PK610_05300"
     CDS             <1..398
                     /locus_tag="PK610_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRJ35380.1"
                     /translation="AHLRYHWMETIVYRTIEYLPLAMIGFGIQDFFIVHMITLSIGHF
                     NHANFTVPLGPLKYIFNNPEMHIWHHARELPNRHGVNFGLSLSIWDYLFKTAYIPHSG
                     RDIALGFEGDEKFPQDFVHQELYGFKKEE"
     gene            449..1165
                     /locus_tag="PK610_05305"
     CDS             449..1165
                     /locus_tag="PK610_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HRJ35381.1"
                     /translation="MKSLIFSFFLFAFTFNIASADPVDHATWDAMLKKYVTAKGNVNY
                     KAWQKDTTDLVAYLKTISDNPPQSSWTPNQKKAYWMNAYNAFTVRLVLARYPVKSIKD
                     IGGIVSPWKLKFFTIAGKKTDLDYIEHSVLRVEFNDPRIHVGINCASYSCPRLSNIAF
                     TADNVEAELEKLMKEFVNDPTKNKITAEKIEISEIFNWFEKDFTKNGSLIDFLNKYSN
                     VKINAGAKKSHMKYNWNLNQ"
     gene            1207..1542
                     /locus_tag="PK610_05310"
     CDS             1207..1542
                     /locus_tag="PK610_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HRJ35382.1"
                     /translation="MEKSYYNPEDLKKFGNIAEFDAELAAKFFDYYGEVFKEGALTAR
                     EKSLIALAVAHAVQCPYCIDAYTQDTIAKGCSEEQMMEAVHVTAAIRGGASLVHGVQM
                     MNKAKKLVM"
     gene            1542..2603
                     /gene="arsS"
                     /locus_tag="PK610_05315"
     CDS             1542..2603
                     /gene="arsS"
                     /locus_tag="PK610_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305816.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HRJ35383.1"
                     /translation="MALIKSLKSRNSDLAEPAEQLKLLNGDIFREGKLPTFREKLMEI
                     NAFPLKPAAIKIFQMNIGYMCNQTCKHCHVDAGPDRKEIMSRETMQRCIDIISEHKFD
                     TVDITGGAPEMNPDFRWFVSELRKTEVREIIVRCNLTIILANAKYNDLPEFFKANRIR
                     VVSSLPFYNADRTDRQRGEGVFAKSVEALKLLNAVGYGLPDSGLILDLVYNPGGAFLP
                     AEQSGLERDFKKELKDHFGIQFNMLLCLNNLPVSRFLEYLVESDNFDEYMDLLVRSFN
                     PGAVQGVMCRDTISVDYDGFIYDCDFNQMLKMKVDRNASQHINDFDIKKLTERNIVIN
                     QHCFGCTAGAGSSCQGATT"
     gene            2669..3124
                     /locus_tag="PK610_05320"
     CDS             2669..3124
                     /locus_tag="PK610_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRJ35384.1"
                     /translation="MLGTQNDFDKMVHSKIRHVVPLIEPSELKKKIESGIEITLLDAR
                     EKSEYTVSHLKNAIHVGYDRFDISSIAKLPKNRLTIVYCSIGVRSEDIAEKLRKSGFA
                     DVKNLFGGIFKWVNNGYPVYSNGNTETNAIHPYSKSWGKWLTKGHKRYE"
     gene            3117..3722
                     /locus_tag="PK610_05325"
     CDS             3117..3722
                     /locus_tag="PK610_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HRJ35385.1"
                     /translation="MSNRTNLLMVFVRNARIGKVKTRLAKKIGDEEALEIYVRLLQYT
                     AEVCGSANAEKAVFYSEYIDESDEFSVSEFQKFLQLGEDLGERMKNSFVKGFGRGYEK
                     IIIVGSDCMELTTEIIDEAFVSLDKNDVVIGPAKDGGYYLLGMKKLYPEFFRNKVWST
                     SNVFLDTLLDIQKMSLKFHALPMLNDVDEESDLTDELRNLL"
     gene            3719..4411
                     /locus_tag="PK610_05330"
     CDS             3719..4411
                     /locus_tag="PK610_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013620388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HRJ35386.1"
                     /translation="MILFELSIIIPVLNEEENIATLIDLLHERSYGISAEIIVVDGGS
                     SDRTMEISLSKNATVISSAKGRPMQMNKGAERASGKILWFLHADSIPPVHYDKFILEA
                     VQSGYLSGCFRMQFQTSNLLLRFFGWLTRFNSEYCRGGDQSLFVTADVFDKSGKYNEQ
                     YLIFEDNEILPRIKKLCKFKVIQHTLFTSIRRYRDNGIYRLQYRFMRLHTMYRRGATI
                     DELLVYYKKHVK"
     gene            4408..5226
                     /locus_tag="PK610_05335"
     CDS             4408..5226
                     /locus_tag="PK610_05335"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRJ35387.1"
                     /translation="MTETEKIKQSAEFLQANGIADVSIGIVLGTGLGNLITKIEIEKE
                     IPYESIPGFPVSTVEFHSGKLIFGKINGKQVLAMNGRFHAYEGYSMKQITFPIRVMKL
                     LGIETLLISNAAGGMNLEFRKGDLMVVADHINLQPDNPLIGKNLDDFGPRFPDMSEAY
                     DKDLRKRLLEIAIRENIKVVEGIYVSVPGPNLETAAEYRFLHRIGADAVGMSTVPEVI
                     VARHQSMRVAAISVITDECDPDHLQPVNIAEIIEVAGKAEVKLSTLFSALIAEL"
     gene            5288..6256
                     /gene="arsM"
                     /locus_tag="PK610_05340"
     CDS             5288..6256
                     /gene="arsM"
                     /locus_tag="PK610_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis arsenite
                     methyltransferase ArsM"
                     /protein_id="HRJ35388.1"
                     /translation="MSYHDTTKDFYKQAALTPDVGLCCTTTPIWKLPELDIPVIMQQM
                     NYGCGSTVHPRDLSGSPTVLYVGVGGGMELLQFAYFSRKKNGVIGIDVVDEMLDASGK
                     NFVEAEQLNPWFKSDFVDLRKGDALHLPVADNSVDVASQNCLFNIFKIDDLKLALQEM
                     YRVLKPNGRLVMSDPISEDVIPDALRNDEKLRAACLSGVIPLQEYIRLITSVGFGTVE
                     IRAKRPYRILDPMHYSTDKQIYIESVEVCAIKDPMPADGPCVFTGKSAIYFGKDEFFD
                     DGKGHVLMQNQPLAVCDKTAGALSDLKRKDIFVSESTWFYDGGGCC"
     gene            complement(6319..>7059)
                     /locus_tag="PK610_05345"
     CDS             complement(6319..>7059)
                     /locus_tag="PK610_05345"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRJ35389.1"
                     /translation="AILPPDDLVKETLPDSFILYRPKDIVSGDFYFVYPVTTSENPPR
                     KLSLFAAVDCTGHGVPGAFMSILGNNILKATLTEKEVNSPADALNYLNRNIISSLSQS
                     KQDVAIRDGMDIALCALESSSGTLHYAGANNPVWVVSKRSGAVECIELNPDKQPIGNY
                     GNNSPFTNHSMKVEKGDMIYVFTDGYADQFGGPKGKKFKYSQLKELLLSIYSLPCPKQ
                     REILEQTFTSWKGNLEQLDDVCLIGVRV"
BASE COUNT         2126 a         1253 c         1563 g         2117 t
ORIGIN      
        1 cagctcacct tcgctaccat tggatggaaa cgattgttta cagaaccatc gaatatcttc
       61 cgcttgcaat gatcggattc gggattcagg attttttcat cgtacatatg atcacgcttt
      121 ccatcggaca ttttaatcat gcaaatttta cagtgccatt aggacctctc aaatacatat
      181 tcaacaatcc cgaaatgcat atctggcatc atgcccgcga attgccaaat cgccatggcg
      241 tgaattttgg cctaagtcta agtatttggg attacttgtt taaaaccgcc tacatcccac
      301 attctggacg agatattgcg ctgggatttg aaggagatga gaaatttccg caagattttg
      361 tacatcagga gctttatggc ttcaaaaaag aggaataatg tttgcatgag aggagggaag
      421 actctatttt tgactaaacc tacccaatat gaaatcgctg attttttcct ttttcctttt
      481 tgctttcacc tttaacatcg catctgcaga tcctgttgat cacgcaacct gggatgccat
      541 gctcaaaaag tatgtaacag ccaagggtaa tgttaactac aaagcctggc aaaaggatac
      601 aacggatctg gttgcttatc ttaaaaccat ttcagataac ccgcctcagt cttcctggac
      661 acccaaccaa aagaaggcgt attggatgaa cgcttacaat gcgtttacag ttcgtctggt
      721 tttagccagg tatcccgtta aaagtattaa ggatattgga ggaatagtta gtccctggaa
      781 actgaaattc tttacgattg ccggaaaaaa aacagatctt gattatatcg aacacagtgt
      841 tctacgggtg gaattcaatg atccgcgtat ccatgttgga atcaattgtg cttcttattc
      901 ttgtccccgc ttatcaaaca ttgcgtttac cgcagataat gtggaggctg aattggaaaa
      961 attgatgaag gagtttgtta acgacccaac caaaaacaag atcacagctg aaaagatcga
     1021 gatctctgaa atctttaatt ggtttgaaaa agattttacg aaaaatggtt cccttattga
     1081 ttttctaaac aagtactcga acgttaaaat caatgccgga gcaaaaaaat cacacatgaa
     1141 atacaactgg aatttgaatc agtaaaaaat agttcttaaa taataattaa tcaaaaaata
     1201 gtcaaaatgg aaaagtcata ttacaatccc gaggatctga aaaaatttgg aaacattgca
     1261 gagtttgatg cagaactagc agcgaagttt tttgattatt acggtgaagt attcaaggaa
     1321 ggtgctttaa ctgcgcgcga aaaatccttg attgcattgg ccgttgcaca tgctgttcaa
     1381 tgtccgtatt gcattgatgc ctatactcag gatacaattg caaagggatg cagtgaagag
     1441 cagatgatgg aagctgtcca cgttactgct gcaattcgtg gtggtgcgtc tttggtacat
     1501 ggcgttcaaa tgatgaacaa agccaagaaa ttggtgatgt aatggcattg atcaaatcac
     1561 ttaaatcgcg taacagcgat cttgccgaac cagcggaaca actgaagctc ttgaacggag
     1621 atattttccg tgaagggaaa cttccaacat tcagggaaaa gctaatggag attaatgcat
     1681 ttccattaaa acctgctgca ataaaaattt ttcagatgaa tattggttac atgtgtaacc
     1741 aaacctgcaa acattgccat gtagacgccg gtcctgaccg taaagagatt atgtcgcgtg
     1801 aaacgatgca gcgctgtatt gatattattt cggagcataa attcgatact gtcgatatca
     1861 ccggtggtgc acctgaaatg aatcctgatt ttcgttggtt tgtttcagag cttcggaaaa
     1921 cagaagtccg ggaaatcatt gtccgctgta accttaccat catccttgcg aatgccaaat
     1981 acaacgactt gcctgaattt tttaaagcga accgcattcg tgtggtgagt agtcttcctt
     2041 tttacaatgc cgatcgtacc gacagacaac gaggtgaggg tgtatttgca aaatctgttg
     2101 aggcgcttaa attattgaat gcagttggat acggtttgcc tgactccgga ttgattctgg
     2161 atcttgttta caatcccggc ggtgcttttt tacctgcgga acaatccgga cttgaacgcg
     2221 attttaaaaa agaactcaag gatcatttcg gaattcaatt caacatgttg ctttgtctca
     2281 acaatctgcc cgttagtcgt tttcttgaat acctggtaga atcggataat tttgatgagt
     2341 acatggatct actggtgcgt tcctttaatc cgggagctgt acaaggtgta atgtgtagag
     2401 atacaatatc ggtagattac gacggattta tttatgattg tgatttcaat caaatgttaa
     2461 aaatgaaagt cgatcggaat gcttcgcagc acatcaatga tttcgatata aaaaagttaa
     2521 ccgaacggaa tattgtgatc aatcagcatt gtttcggatg cacagcagga gccggttcga
     2581 gttgtcaggg tgctactaca tgatcaatcg taaattcatt tccattggga tttttctgat
     2641 cctgacagtt ttcctggaat cattttttgt gttaggaacg caaaatgatt tcgataaaat
     2701 ggtgcatagt aaaatccgac atgttgtacc actgattgaa ccttccgagc tgaaaaagaa
     2761 aattgaatcg ggtattgaaa ttacgcttct cgatgcccgg gaaaaatctg agtatacagt
     2821 cagccatttg aaaaatgcca ttcatgttgg ctacgatcgc tttgatatca gcagcattgc
     2881 taagttgccc aaaaacagat taaccattgt ttattgcagt attggtgttc gctctgagga
     2941 tattgcggag aaattgcgga aatcagggtt tgcagatgtg aaaaacctct ttggtggaat
     3001 ttttaaatgg gttaataacg gttatcctgt ctattccaac ggcaatacag aaaccaatgc
     3061 cattcatcct tacagcaaat catggggtaa atggcttaca aaaggacata agcggtatga
     3121 gtaacaggac caatctatta atggtttttg ttcgaaatgc caggataggt aaggtgaaaa
     3181 cgcgtcttgc aaaaaaaatt ggcgacgaag aagcattgga aatatatgtt cgtttgttgc
     3241 aatataccgc tgaagtttgt ggttctgcta atgcagaaaa agctgtcttc tattcagagt
     3301 atatcgacga aagcgatgaa ttttcggtgt ctgaattcca aaaattcctt cagttagggg
     3361 aagatcttgg agaacggatg aagaattcat ttgtaaaagg ttttggcagg ggatatgaaa
     3421 aaattataat tgtcggaagc gattgcatgg agttaacaac ggagatcatc gatgaagcgt
     3481 ttgtttcgct tgataagaat gatgttgtga tcggccctgc aaaggatgga ggttattatt
     3541 tgttggggat gaaaaaatta taccccgaat ttttcagaaa taaggtttgg agtaccagca
     3601 atgtttttct ggatacactt ctcgatatcc aaaaaatgag cttaaaattt catgccttac
     3661 ccatgttgaa cgatgtggac gaagaatcgg atcttacgga tgaactcaga aatctgttat
     3721 gattcttttt gagctgagca tcattattcc tgtcctaaat gaggaagaaa atattgcaac
     3781 actaattgat ttgttacacg aacgttcgta cggtatttcc gcagaaataa ttgttgttga
     3841 tggtggaagc agtgatcgga ccatggagat ttccctttca aaaaatgcta ctgtgatttc
     3901 ttctgccaaa gggcgtccaa tgcaaatgaa taaaggtgca gaacgtgctt caggtaagat
     3961 tttatggttt ttgcacgcgg acagtattcc accggtgcat tacgataaat tcattcttga
     4021 ggcggttcaa tccggttatt tatcaggttg tttcagaatg caatttcaga caagtaatct
     4081 tttgcttcgg ttctttggtt ggcttacccg attcaattcg gagtattgca ggggaggaga
     4141 tcaaagttta ttcgtaacag ctgatgtatt tgataaatca ggaaaataca atgaacagta
     4201 tttgattttt gaggataatg aaattctgcc gcgaataaaa aaactttgta agtttaaggt
     4261 aattcaacat acacttttta cttcaatcag gagatacagg gacaatggga tttatcggct
     4321 tcaataccgg tttatgcgct tgcatacaat gtacaggcga ggagctacga ttgacgaatt
     4381 gctggtgtat tataaaaaac acgtaaaatg acagaaacag aaaaaataaa gcaatcagct
     4441 gagtttttac aggccaatgg aattgcagat gtttcaattg gaattgtgct tggtaccgga
     4501 ttgggaaatt tgattacaaa aattgagatc gaaaaagaaa ttccttacga gtccatacct
     4561 ggttttcctg tgagcacggt ggaatttcat tccggaaaat tgattttcgg aaagatcaac
     4621 gggaagcagg tattggccat gaacggtcgg tttcatgcct acgaaggcta ttcaatgaaa
     4681 cagattactt ttcctattcg ggtgatgaag ttattgggaa ttgaaaccct cttaatttca
     4741 aatgcggctg gtggaatgaa tttggaattt cgcaaaggtg atttgatggt tgtcgcggat
     4801 catatcaacc ttcagccgga taatccctta ataggaaaaa accttgatga ttttggtcct
     4861 cgttttccgg atatgagcga agcttacgat aaagatctga gaaaacgttt gttggaaatt
     4921 gcaataaggg agaacataaa agttgttgag ggaatttatg tttcagttcc cggtcccaac
     4981 cttgaaactg cagcagaata ccgcttttta caccgtattg gtgcggatgc agttggtatg
     5041 agtacagtcc cggaagtaat agttgcccgt catcaatcca tgagggttgc ggctatttcg
     5101 gtgattacgg atgagtgtga tcctgatcat cttcaaccgg tcaatattgc tgaaataatt
     5161 gaagtggccg gaaaagctga agtgaaactt agtactttgt ttagtgcttt aattgcagaa
     5221 ttgtaagtat tgattgaatg cactaaactt ctacagatca tttgtatttt aatttgaaca
     5281 ctgaacgatg tcttaccacg ataccacgaa agatttttac aagcaggcag cacttactcc
     5341 cgatgttgga ctttgttgta ccactactcc catttggaaa cttcctgaat tggatattcc
     5401 tgtgatcatg caacaaatga attacggatg cggaagtact gttcaccctc gtgatctttc
     5461 cgggtcgcca actgtcctgt atgttggcgt tggtggcgga atggagttac ttcagtttgc
     5521 atatttcagc cggaaaaaaa acggagtcat tggaattgat gtggtggatg aaatgctgga
     5581 tgcttcggga aagaattttg tggaggctga acaattgaat ccatggttta agtctgattt
     5641 cgttgatttg cgaaaaggtg acgcattgca cttaccggta gcagataatt ccgttgatgt
     5701 ggcctcacag aattgcttgt tcaatatttt taagatcgat gatctgaagt tggctttgca
     5761 ggaaatgtac agggtattga aaccgaacgg acgattggta atgagtgatc cgatcagtga
     5821 agatgttatt cctgatgcat tacgaaacga tgaaaaactc agagctgcat gtcttagcgg
     5881 tgtcatccct ttgcaagaat atatccgatt gattacttca gttggatttg gaaccgttga
     5941 aatcagagcc aaacgtccat accgaatcct tgatccgatg cattattcta cggataagca
     6001 gatttacatt gagagtgttg aagtatgtgc aatcaaagat ccaatgccag cggatgggcc
     6061 ttgtgttttt acaggaaaat cggccattta ttttgggaag gatgaatttt ttgatgacgg
     6121 aaaaggtcat gtgttgatgc aaaaccaacc tcttgcggta tgtgataaaa ctgctggcgc
     6181 tttgagtgat ttgaaaagga aagatatttt tgtttccgaa tcaacctggt tttatgatgg
     6241 gggaggctgt tgttaggatg ttgcaaatct gaatgtataa aaggtaaaat gcgaagctaa
     6301 attgttgcat tacacatttt atactctgac accaattaaa caaacatcat ccaattgctc
     6361 gaggtttcct ttccaggaag taaacgtttg ttccaaaatt tcccgctgct tcggacaggg
     6421 gagggaataa atactcagta gtaattcttt cagttgactg tatttaaatt tctttccttt
     6481 tggtccgcca aactgatcgg cgtagccatc ggtaaagacg tagatcatat ctcctttttc
     6541 aactttcatg gaatggttgg taaatggaga attgtttccg taattaccaa ttggttgttt
     6601 atccggattc agttcaatac attcaacagc accggatctt ttggaaacaa cccaaacggg
     6661 attgttcgct cctgcataat gaagagtgcc actactgctt tccaaggcac ataaggcgat
     6721 gtccattcca tcgcgaatag ctacatcttg tttggattgc gatagagagg aaataatatt
     6781 tctgttcaga taatttaaag catcagcagg agagttgact tccttttcag ttaatgtggc
     6841 tttcaggata ttgttaccca aaatcgacat aaaggcgccc ggaaccccgt gccctgtaca
     6901 atcaactgca gcaaaaagcg ataattttcg tggaggattt tctgaagtgg taacaggata
     6961 cacaaaatag aaatcgccac ttacaatatc cttaggtcgg tataaaataa atgaatccgg
     7021 tagagtctct tttaccaaat catccggtgg aagaatggc
//
LOCUS       DAOMYG010000122         7058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3596_length_7058_cov_9.941882, whole genome shotgun sequence.
ACCESSION   DAOMYG010000122 DAOMYG010000000
VERSION     DAOMYG010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7058
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3596_length_7058_cov_9.941882"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1129)
                     /locus_tag="PK610_05350"
     CDS             complement(182..1129)
                     /locus_tag="PK610_05350"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRJ35390.1"
                     /translation="MNALTATDFKFPGQKSFYKGKVRDVYNINDQFLVMVVSDRISAF
                     DVVLPKGIPYKGQVLNQLAGHFLSATSDIVPNWVIAQPDPNVTIGHLCEPFKVEMVIR
                     GYLAGHAAREYKAGKRILCGVKLPEGLRENDKLPEPIITPTTKASEGHDEDISREEII
                     AKGIVSEEDYVQLEKYTRAVYQRGVEMAAEKGLILVDTKYEFGKKDGKIYLIDEIHTP
                     DSSRYFYMEGYEERQAKGEEQKQLSKEFVRQWLIAGGFQGKDGQQIPEMDDAFVSSVT
                     ERYIELYEKVSGKKFERGTTTAIEQRVYDNVMRFLKATV"
     gene            complement(1141..2214)
                     /locus_tag="PK610_05355"
     CDS             complement(1141..2214)
                     /locus_tag="PK610_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRJ35391.1"
                     /translation="MKEKVFEINAINPIELFGVNDAKLDLIRKHYPKLKLVARGTTLK
                     AIGDQDNLESFEKKLELLIEHYNKYHRLTESNIEQLLLDDSSEAVKPTGGDEVLVYGN
                     SGLQIKARTPNQRKLVEKMAEHDMVFAIGPAGTGKTYTAVALAVRALKNKEVRRIILT
                     RPAVEAGENLGFLPGDLKEKLDPYLQPLYDALREMLPPEKLREYLEQGTIQIAPLAFM
                     RGRTLDKAFVILDEAQNATEAQMKMFLTRMGMSAKFVITGDVTQVDLPRHQPSGLIRA
                     IRVLQDIETIGFVFLDGRDVIRHKLVKYIIDAFEKKGNTADEAENPPKGRIGGQEKKE
                     EHRDAVKTKKTTSRTPIRKSARK"
     gene            2275..3129
                     /locus_tag="PK610_05360"
     CDS             2275..3129
                     /locus_tag="PK610_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HRJ35392.1"
                     /translation="MFSKIAISRMAIITLTSDMGDRDFYVAAVKGAILSRLPDSRIVD
                     ISHHIAPFDIAQASFVLRNSYPSFPQGSIHIIGINPEKISRKGPFDMRDEAGHLVVRH
                     KGHTFIGADNGIFSLLFDEAPEEVFEITIQLNEDPGGFPMKNVFAPIACLLAAGTPLE
                     EIGLPHAEIRERAVFRPVVESGMLKGTVIYIDFYGNVITNITRELFEQVRKGRKFTLL
                     FRSEDYEINEISSTYGDVPEGEKLALFGASGHLEIAINKGVEGSGGGASGLFGLKLND
                     TVRIEFED"
     gene            3303..3998
                     /locus_tag="PK610_05365"
     CDS             3303..3998
                     /locus_tag="PK610_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35393.1"
                     /translation="MEFLMNRQFGPTPSDTLSDEPEIVWYYKAYISKYDLNQRLFSTS
                     ILDTGIVNFEDKTVLAKLKTVFSTGMKQIKTNQPAIKNLTPEYLSLCDFQQKCNIISL
                     VHNKNLDSSFIVYNKKSYFIDIPRDSLHNEAYRNMEGIRISSLQVSSIRIFRHERKKL
                     IFIHLGTGDEIHMGYLTDDASKKGQTIDGFPITICKENKPSFPFTTLSNSVYIEPILH
                     HGFGVDAFLVLSD"
     gene            complement(4068..6194)
                     /locus_tag="PK610_05370"
     CDS             complement(4068..6194)
                     /locus_tag="PK610_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HRJ35394.1"
                     /translation="MEKIISLIANRIGLRVPQVVNTVKLLDEGATIPFISRYRKEMTG
                     SMDEVEVMRVKFEYDKLKALVTRKAGILETIEEQGKLTPELKAKIEACYDPTDLEDLY
                     LPYKPKRKTRSVVAREKGLEPLALKLFAQKDNDVESLAENFLSDEVKTVEDALEGARD
                     IIAEMISEDATVRNKIRFLYSREAQVSSKLVKGKDLDGAKFKDYFDWKEALSRIPSHR
                     LLAMFRGENEGFLRVRIGVDKEEALEVIERHVVKSRNESADQVKDAIADCWSRLLEPS
                     IETEFRISSKEKADEGAVKVFAENLRQLLLSPPLGQKRILALDPGFRTGCKLVCIDEK
                     GDLIHNETIYPHPPQSETADAMRTLSRLVEQYKIEAISIGNGTASRETEQFVKRCRFD
                     REVKVFVVSEAGASIYSASSVAREEFPSYDVTVRGAVSIGRRLMDPLAELVKIDPKSI
                     GVGQYQHDVDQGMLKRELDAVVESCVNNVGVSLNTASKHLLTYVSGLGPILAENIVEF
                     RSKNGPFRSREDLKQVPRLGPKAFEQSAGFLRVEDSANPLDNSAVHPESYAIVEKMAK
                     KLGKQVKELIGNDTITKQLKADEFITEKAGKETVQDIIKELAKPTRDPRKAAKVFEFA
                     DGIFKMEDLREGMIVPGIVTNITNFGCFVDVGVKQDGLVHVSQLANRYVSDPNEVVKL
                     HQHVKVKVMEVDIPRKRIGLSIKEAE"
     gene            complement(6375..6854)
                     /locus_tag="PK610_05375"
     CDS             complement(6375..6854)
                     /locus_tag="PK610_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35395.1"
                     /translation="MIKYFFIAFLFVPVYSNGQSWTDADKKQLKKDCMKDAVKALIPG
                     AANDYCSCYVEGFLSRFPLKTTPVSDTALMVIANRCIEEVKYRYPGGPFIDRWNDPAK
                     ASFISGCKQKLLNVKMDADAYCQCMLVKVMEVQPDPRLVNAIDPLILDKIASACLGR"
     gene            complement(6854..>7058)
                     /locus_tag="PK610_05380"
     CDS             complement(6854..>7058)
                     /locus_tag="PK610_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35396.1"
                     /translation="SVTNPSIFLEAGVSLKLRLKFFFFGGTAGWQLDVSKKGWRENGS
                     YVSGGPKTSQSGMVIMGQAGFYF"
BASE COUNT         1884 a         1717 c         1414 g         2043 t
ORIGIN      
        1 gaaaagctat ttttccaatc ctgaagctga aaaggaagaa aagttcaaat tactgcaaac
       61 cattaccacc atcatcaagg tgcacgatga taaccagatt ctggattgat cttcgttttc
      121 caaaacaaca attcctgctt tgattttttt ccgggtgaaa tttcaggcgt actgaaagtt
      181 cttacaccgt cgcctttaaa aagcgcatca cattatcgta cacccgctgc tcaatggcag
      241 tagttgttcc gcgttcgaat ttctttccgc tcactttttc atacagttcg atatagcgct
      301 cggttacgct gcttacaaaa gcatcgtcca tttccggaat ctgttgaccg tctttcccct
      361 gaaatccgcc tgcaattaac cactgacgca caaactcctt ggagagttgt ttttgctctt
      421 ctcctttggc ctgacgttct tcgtagcctt ccatatagaa ataacgcgat gaatcgggag
      481 tatgaatttc atcgatcagg tagatcttcc cgtccttctt tccgaattcg tatttggtat
      541 cgaccagtat caatcccttt tcagcagcca tttctactcc acgctgataa acggcgcgtg
      601 tgtatttttc gagttgcaca taatcttctt cgctcacaat tcccttggca atgatctcct
      661 cgcgcgaaat atcttcatcg tgtccttcgc tggctttggt ggttggtgta atgataggtt
      721 caggtaattt gtcgttttcc cttaatcctt ccggcaattt caccccgcac aaaatgcgtt
      781 ttcctgcttt gtattcgcgt gccgcatggc ctgcaagata tccgcgtatg accatctcca
      841 ctttaaaggg ttcgcataaa tgtccgatcg ttacattcgg atccggttgt gcgatcaccc
      901 agttgggaac gatatcggaa gtggccgata aaaaatgtcc ggcaagctga ttgagcactt
      961 gtcctttgta aggaattccc ttgggcaaca ccacatcaaa agcagaaatg cgatcgctca
     1021 ccaccattac caggaactga tcgttgatat tgtacacatc gcggactttt cctttgtaga
     1081 aacttttttg tccgggaaac ttaaaatctg tagcggtgag tgcgttcata gttgttgcca
     1141 ttattttctt gctgatttac gaatgggagt tcgtgatgtc gttttttttg tttttacagc
     1201 atctctgtgc tcttcttttt tctcttgtcc gccgatgcgt cccttaggag gattttccgc
     1261 ttcgtctgct gtatttcctt ttttctcaaa tgcatcgatg atgtatttca ccagtttatg
     1321 gcgaatcaca tcgcgaccat cgagaaacac aaatccgatg gtttcaatat cctgtaatac
     1381 acgaatagcg cgaatcaatc cggaaggctg gtgacgtgga agatcgacct gtgtgacgtc
     1441 gccggtaatg acaaacttcg cactcattcc catacgggtg aggaacattt tcatttgtgc
     1501 ttcggtagcg ttttgcgctt catccagaat aacgaaagcc ttatcaagcg ttcttccacg
     1561 cataaaagcc agtggagcga tctgaatggt tccctgttcg aggtattcgc gtaatttttc
     1621 gggcggcagc atttcgcgca aggcatcgta gagcggttgc agataaggat cgagtttttc
     1681 cttgagatca cccgggagga aacccagatt ctcaccggcc tcaacagcag gacgtgtaag
     1741 aatgattctt cgcacctcct tgttcttgag tgcacgcaca gcaagcgcta cggcagtgta
     1801 ggttttacct gtacctgcag gtccaatcgc aaacaccata tcgtgttccg ccattttctc
     1861 caccagttta cgctgattgg gagtacgcgc cttgatctgc aatccggaat tgccatagac
     1921 caacacctca tcgccacctg taggtttcac cgcttcagaa gaatcgtcga gcagaagctg
     1981 ttcgatattg ctttcggtga ggcgatggta tttgttgtag tgctcaatga ggagctccag
     2041 tttcttctca aacgattcca ggttatcctg atcgccaatt gccttgagcg tggtgccacg
     2101 ggcaacgagt ttgagttttg gataatgctt gcggatgaga tcaagtttgg catcatttac
     2161 cccgaataat tcgatgggat tgattgcatt gatctcaaaa actttttctt tcaattgaat
     2221 tggatttaga tcacagcaca caaaaagtgg gcttgctgtt tttttggatt acttttgttt
     2281 tcaaaaatag caatttcccg aatggccatc atcaccctca cctccgatat gggcgaccgc
     2341 gatttctacg tggccgcggt gaagggtgcc attctcagcc gcctgcccga cagcaggatc
     2401 gtggatatct ctcaccacat agcccctttc gacattgcac aggcctcctt tgttctccgc
     2461 aactcctacc cttcattccc gcagggcagc attcatatca ttggcatcaa ccccgaaaaa
     2521 atcagcagaa aagggccttt cgatatgcgc gatgaagcag gacacctggt tgtcaggcac
     2581 aagggtcaca cctttatcgg ggccgacaat ggtattttct ccctgctttt cgacgaagcg
     2641 ccggaggagg tttttgagat taccatccaa ctgaacgaag atccgggagg ctttcccatg
     2701 aaaaatgtgt tcgcccccat tgcctgtctg cttgctgccg gcacaccact ggaggagatc
     2761 ggactacccc atgcagaaat ccgcgaaaga gccgtttttc gtcccgtggt agaatccggc
     2821 atgctaaaag gcactgtaat atacatcgat ttctacggca acgtgatcac caacatcacc
     2881 cgcgaattgt ttgagcaagt gcggaaggga cgcaaattca ccctgctttt ccgcagcgaa
     2941 gattacgaga tcaacgagat aagcagcact tatggcgatg ttcccgaagg agagaagctc
     3001 gcactctttg gcgcatccgg tcacctggaa atcgctatca acaaaggcgt agaaggctca
     3061 ggcggcggag cgtcaggatt attcggcctg aaactgaatg atacggtgag gatagagttt
     3121 gaagattaaa agacgttata cccttgtatt caactcaatt ctgaataata aaaacgatca
     3181 cgatgaaatg gctgcctctt ttgcttctat gtttctgttt gcccgtggat ggtaatagct
     3241 gtttaaacgc atttcagcat aaaaattttc ctgttggggt gtattctgat acactctttt
     3301 gcgtggaatt cttaatgaat agacaatttg gaccaacacc ctcagatacc ttatctgatg
     3361 aaccggaaat tgtatggtac tacaaggcat acatttcaaa atacgacctg aaccaacggc
     3421 tcttttccac ttctattttg gacacaggca tcgtaaattt tgaagacaaa acagttcttg
     3481 caaaacttaa aacagttttc agcacgggga tgaaacaaat caaaaccaat caacctgcaa
     3541 taaaaaacct tacaccggaa tatctttcct tatgcgattt tcaacagaag tgcaatatta
     3601 tctcgttagt gcataataaa aacctggact cttcatttat agtttacaat aaaaaatcct
     3661 atttcatcga tataccaaga gatagtttac acaatgaagc ctacaggaat atggaaggaa
     3721 taagaataag ttcacttcag gtcagttcga tccgaatatt taggcacgaa agaaagaaac
     3781 tgatctttat acaccttggc acaggtgatg aaattcacat gggttatcta accgatgatg
     3841 cttctaaaaa agggcaaaca atagatggct ttccaataac aatctgtaaa gagaataaac
     3901 catcattccc gtttacaaca ctttcaaatt cggtttatat tgaaccaata ttacatcatg
     3961 gtttcggcgt tgatgcattc cttgttctga gcgattaata caacaacatc caattaaaaa
     4021 agcccaatga aattcacagg gcttttccta taaaaaaatc tattcgtcta ctccgcttcc
     4081 ttaatggata atccaattcg cttgcgggga atatccactt ccatcacttt cacttttaca
     4141 tgctggtgca gcttaaccac ttcattggga tcgcttacat aacggttcgc caattgagaa
     4201 acatgcacca aaccatcctg tttcactccc acatccacaa aacaaccaaa attggttatg
     4261 ttggtaacaa tgccaggaac gatcattcct tcgcgcagat cttccatttt aaaaatacca
     4321 tcggcaaatt caaacacctt cgctgctttg cgcggatcgc gggttggttt tgccaattcc
     4381 ttgatgatat cctgcaccgt ttcctttcct gctttttccg ttatgaattc atcggctttt
     4441 aattgtttgg tgatggtatc gttaccaatc agctctttta cctgcttgcc cagttttttc
     4501 gccatcttct ccacgatcgc ataactctcc gggtgaactg cagaattatc aagaggattc
     4561 gcactgtctt ccacacgcaa aaatccggca gattgttcaa atgctttcgg acccaagcgc
     4621 ggtacttgtt tcagatcttc acgcgaacgg aaaggaccat tcttcgatct gaactctaca
     4681 atattttctg caagaatagg acccaatccg gaaacgtaag tgagcaaatg tttggaagcg
     4741 gtattgagcg aaacgcctac gttgttcacg caactttcca ccaccgcatc cagttcgcgc
     4801 ttgagcattc cctgatcaac atcgtgctgg tactgcccta ctccaatact tttcggatcg
     4861 atcttcacca actcagcaag cggatccatc agtcggcgac cgatagaaac tgcaccgcgt
     4921 acggtaacat cgtaactcgg aaattcttcg cgcgccaccg atgatgcaga ataaatggaa
     4981 gcaccagctt cactcacaac aaatactttt acctcgcgat cgaaacgaca acgcttaaca
     5041 aattgctccg tttcgcgact ggcggttccg ttgccaatgg agatcgcttc aattttatat
     5101 tgctccacca agcgcgataa agtacgcatg gcatcggctg tttcactttg cggcggatga
     5161 ggataaatgg tttcgttatg aatcaaatct cctttttcat caatacacac caatttacat
     5221 ccggttctga atccgggatc aagcgcgaga atacgttttt gtcccaatgg cggacttaac
     5281 aacaactgac gtaaattctc cgcaaatact tttactgctc cttcatcggc tttttccttg
     5341 gatgaaattc taaattccgt ttcaatcgaa ggctcgagta aacgactcca gcaatcggca
     5401 atggcatctt ttacctggtc agcactttca ttgcgcgatt ttacgacgtg acgttcgatt
     5461 acttccaacg cttcttcttt atcaacaccg atgcgaacac gcaaaaaacc ttcgttttct
     5521 cctctgaaca ttgccagcaa acggtgtgat ggaatgcgcg acaatgcctc tttccaatcg
     5581 aaataatcct tgaattttgc accatccaaa tcttttccct tcaccagttt ggaggatacc
     5641 tgtgcctcgc gtgaatacaa aaaacgaatt ttgtttcgca cagttgcatc ttcgctgatc
     5701 atctccgcaa taatgtcacg cgcaccttcc aatgcatctt ccactgtttt aacttcatcc
     5761 gaaagaaaat tctcggcaag cgattccaca tcgttatctt tttgcgcaaa taattttaaa
     5821 gcaagtggct ccaatccttt ttcgcgggca acaacggatc tggtttttcg cttgggctta
     5881 tagggcagat agagatcttc aagatcggta ggatcataac aagcttcaat tttcgctttc
     5941 aattcaggtg tcagctttcc ttgttcttca attgtttcaa gaatgcctgc cttgcgcgta
     6001 accagtgctt tcagcttatc gtattcgaat tttacacgca tcacttccac ttcatccata
     6061 ctgccggtca tctctttacg gtaacgggaa atgaaaggga tggtcgcgcc ttcgtcaagc
     6121 aatttaaccg tattcaccac ctggggaaca cgcaatccga ttcggttagc aataagagaa
     6181 atgatctttt ccatgacttg tgtttgttcc gcaataataa gataaaggca gattgcacat
     6241 gggaaaactt attgacctca gcgaaaaaac ttgtttaaaa attgtactcg ctcttaataa
     6301 gtttaaacaa gcggatttgg aagtaatacg ggaaattcta agtgattccg aaaataaagg
     6361 aaatgtttcg caaactatct accgagacat gcagaggcga tcttatcgag aatcagcgga
     6421 tcaatggcat tcaccaatcg gggatcgggt tgaacttcca ttacttttac cagcatgcac
     6481 tgacaatatg cgtctgcatc catttttacg ttcagcagtt tctgtttgca gccggaaatg
     6541 aaggatgcct tggcaggatc gttccaacgg tcgataaatg gtccgccggg ataacggtat
     6601 tttacttctt cgatacatcg attggcaatt accatcaaag cagtatccga aaccggtgtc
     6661 gttttcagcg ggaagcgcga caaaaatcct tcaacatagc aactgcaata atcatttgca
     6721 gcaccgggaa taagtgcttt caccgcatct ttcatgcagt ctttcttcag ttgcttttta
     6781 tctgcatctg tccagctttg tccgttcgaa taaaccggaa caaacaaaaa cgctatgaag
     6841 aaatacttaa tcatcaaaaa taaaatcctg cctgacccat tattaccatt ccgctttgcg
     6901 atgtttttgg tccgccggaa acatacgatc cgttttcgcg ccaccctttt ttcgaaacat
     6961 ccaactgcca acctgcagtt cctccgaaaa agaaaaattt caaacgcaat ttcagcgaaa
     7021 cgcctgcttc aagaaaaatg gatggattgg ttacactt
//
LOCUS       DAOMYG010000123         6949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3675_length_6949_cov_9.719031, whole genome shotgun sequence.
ACCESSION   DAOMYG010000123 DAOMYG010000000
VERSION     DAOMYG010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6949
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3675_length_6949_cov_9.719031"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..970
                     /locus_tag="PK610_05385"
     CDS             230..970
                     /locus_tag="PK610_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35397.1"
                     /translation="MKTLSTSVYATPYFRNTFVATLLFLIGTFLFKDVKSITPSTELP
                     SGLIFRIKTERNDTLLNTEEKIAFSPSFITQHECEFGIITDYIRAQKTKNMLRDQGFL
                     KTEIVAYFNRSPISIDDAFLLLNNRNSSEIKITSSISIREMDSLLGLLQKEEPYFAIS
                     ISMNDAQTVNRFFNLSGKLDIRVNLNGTKEIVFGKFSTIDDANVYLELLTDQGIVSTS
                     ISAWSQSGRAIPIERVLSGSDAPDIAGQ"
     gene            1228..1494
                     /locus_tag="PK610_05390"
     CDS             1228..1494
                     /locus_tag="PK610_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35398.1"
                     /translation="MMRGRHSAVTDPNLKVQVGTGMLYISYPCRTSNVQYNIATPNGK
                     VAIKGDFDNEADDNNIDISRLNSGNYTLYIIDGADLIKYPFSIK"
     gene            complement(1537..2325)
                     /locus_tag="PK610_05395"
     CDS             complement(1537..2325)
                     /locus_tag="PK610_05395"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ35399.1"
                     /translation="MTPHFCTYNGKKIRYYTEGAGPVVVLLHGFLESSFIWHYFSKQL
                     SQRFKIISIDLPGHGSTDVFSEVHTMPLMADVVVAVLKQEKEKKAKVLGHSMGGYAAL
                     SFAERYADYCEGIGLINSTAFPDTEEKKADRLRAIRVLEMSPAVFIREAIPNLFAPFN
                     VERFSEEIEMIRTEALKTSVYGASACLRGMKDREDKTGLIRSGKFPVLFIAGKYDNVI
                     AANLSVEQMELHPSVSGLLLENSGHMCFLEEREKTLEAIREFVG"
     gene            complement(2322..3614)
                     /locus_tag="PK610_05400"
     CDS             complement(2322..3614)
                     /locus_tag="PK610_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013620971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HRJ35400.1"
                     /translation="MKYLPLNAKLFVDNRKRFAAMLKPGSIAVFNSNDLLPTNADGHM
                     TFRQNNDLFYLSGIDQEESILVLFPFAHDPAHREVLFLRETNEEIAIWEGAKHSKRSA
                     YETSGIKTVYWLSQFDRVFKMLAFEAETICLNTNEHLRAHIETQTRDARFIQWIKEHF
                     PLHSLERVAPLMHQIRSVKSGLEIELMQTAIDITEKAFRRILGYIKPGVMEYEIEAEI
                     IHEFVRNRSRGFAYTPIIASGFNACVLHYIDNNAECKDGDVILLDVGAEYANYNADMT
                     RCVPVNGKFTKRQKDVYNAVLRVMRESMKMLRPGTMLADYHKEVGKIMEKELTGLGLL
                     SETDVKNQDPSMPAYKKYFMHGTSHFLGLDVHDVGLWHKPIQPGMVFTVEPGIYIREE
                     NLGIRLENNVMVTESDPRDLMAAIPVEADEIEALMKKK"
     gene            complement(3734..5077)
                     /gene="accC"
                     /locus_tag="PK610_05405"
     CDS             complement(3734..5077)
                     /gene="accC"
                     /locus_tag="PK610_05405"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRJ35401.1"
                     /translation="MFHKILIANRGEIALRVIRTCKEMGIQTVAVYSTADKDSLHVRF
                     ADEAVCIGPPPSKDSYLNMSRIIAAAEITNADAIHPGYGFLSENSKFSKICQDHNIKF
                     IGATPYQIDSMGDKASAKDTMKKAGVPVIPGSDGLLADMAEAKKIAAKIKYPIILKAT
                     AGGGGKGMRVVWKEEDLEAAWDSARQEAGAAFGNDGVYMEKFIEEPRHIEIQVAGDQF
                     GNACHLSERDCSIQRRHQKLVEETPSPFMTPELRKKMGDAAIKAVKAIKYEGVGTIEF
                     LVDKHRNFYFMEMNTRIQVEHTITEEVIDYDLVKEQIKIAAGVAISGKNYEPQLHAIQ
                     CRINAEDPFRNFAPSPGKITNYHSPGGHGIRIDTHVYAGYVIPPNYDSMVGKLITVAR
                     TREEAITKMQRALDEYIIEGIKTSIPFHQALLKNEDFRAGNFTTKFVEDFWNPGK"
     gene            complement(5201..5686)
                     /gene="accB"
                     /locus_tag="PK610_05410"
     CDS             complement(5201..5686)
                     /gene="accB"
                     /locus_tag="PK610_05410"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRJ35402.1"
                     /translation="MNLNEIQDLIKFVSKSGVTEVEIEKKDFKIVIRAKAAIVQAAGP
                     VVTQVAAPAQQMIAAAPVTAAPQAEAPVAPAAGGDDSKYVTIKSPMIGTFYRSPGPEK
                     PVFVNVGDTIKAGDKICIIEAMKLFNEIEAEISGKIVKVLMDDAKPVEYDQPLFLVEP
                     A"
     gene            complement(5709..6707)
                     /locus_tag="PK610_05415"
     CDS             complement(5709..6707)
                     /locus_tag="PK610_05415"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020215091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRJ35403.1"
                     /translation="MSRITAAITAIGGYVPEYVLTNEELSTMVDTNDEWIKSRTGISE
                     RRILKGEGKALSDICVEAVKQLLSKRGIGPEDIDLLIVGTVTSDYNFPSTSNVVCDKL
                     GAKNAWGYDLSAACSGFIYALATGAQFIETGRYKKVVVVGADMMSSILDYTDRTTCVI
                     FGDGGGAVLLEPDTEGYGVKDFILRADGSGRKYLCQPAGGSLKPASHETVDNKQHYVY
                     QEGKAVFKFAVTNMADVSAEIMEKNKLTADDVTWLVPHQANLRIIDATAERMGLDKAK
                     VMINIQKFGNTTAGTIPLLLWEWESKLRKGDNLILSAFGGGFTWGALYLKWAYNSN"
     gene            complement(6704..>6949)
                     /locus_tag="PK610_05420"
     CDS             complement(6704..>6949)
                     /locus_tag="PK610_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HRJ35404.1"
                     /translation="NVVLKEAEGLYWLMRKRGIRDEYFDRFNYENYGGTPVLGVNSNV
                     IIGHGISNATAIKNMILLSRDVVKAGIPEKIKGVFAK"
BASE COUNT         1913 a         1646 c         1391 g         1999 t
ORIGIN      
        1 agttttgtat gcacgcatac caatttaggt ctgtttggat tttccattcg tcgaaaaaaa
       61 acaactcttt gcccttttat cctgtaaaaa caaccaaaca ccagcctgat cccgcccgaa
      121 gagatcagga accagagcta gcaatgacat tagaagcttc gtgaagcccc tttattgtaa
      181 ggggatggat ccggcccatt gccgggagtt gaactgtaat gcttcagtta tgaaaacgct
      241 ttctacttcc gtttatgcga caccttattt cagaaatact tttgtcgcta ctcttttgtt
      301 cctgattggt acttttcttt tcaaagatgt aaaatcaatc actccctcaa cagaacttcc
      361 ttcgggattg atcttccgca tcaaaacaga gaggaatgat accttgttga atacagaaga
      421 aaaaattgct ttttcacctt ctttcataac gcaacacgaa tgtgaattcg gtatcatcac
      481 cgattacatc cgggctcaga aaacaaaaaa catgctgcgt gatcagggat tcctgaaaac
      541 tgaaattgtt gcctatttta atcgcagtcc gatcagcatt gatgatgcat tcctattatt
      601 gaacaatcgc aattcatcgg aaataaaaat tacttcttca attagtatcc gggaaatgga
      661 ttcactactt ggtctcttac aaaaagaaga gccctatttc gccatctcca tctcgatgaa
      721 cgacgcacaa accgtgaacc gcttcttcaa cctcagcgga aaactcgata tacgtgtaaa
      781 ccttaacgga accaaagaaa tcgtattcgg taaattctca accattgatg atgcaaacgt
      841 atacctcgag ttattgacgg accagggcat cgttagcaca tcaataagcg catggagcca
      901 atccggacgt gcaattccaa ttgaacgcgt cctgagtggc tcagacgcac cagacattgc
      961 cggtcaatga ataccaattc cgaacaaaac aaagggatgg gccttccatc cctttattgt
     1021 tggtacaatc tttgctaaac cgtgttagac aaggattttt taaaaaatga agcagaaatt
     1081 caacgattgt aaagggcggt tggtgggcca attttggggc ttaggcaaaa tacccattta
     1141 tagtggcgtc gaaacaacaa ccatttgcgt cttttgtatg ttcaatacca catcgcaagg
     1201 aaacaaatgg ccacaatttt acaaagcatg atgagaggaa gacattcggc tgttaccgac
     1261 cctaacctta aggttcaggt aggaacagga atgttgtaca tcagctatcc ttgccgtact
     1321 tcaaacgtcc aatacaacat cgccacaccc aatggaaaag ttgcgataaa aggtgatttt
     1381 gacaatgagg ccgacgataa caacattgat atctccagat taaacagcgg gaattacacg
     1441 ctgtatataa ttgacggcgc cgacctgatc aaatatccat tttccattaa gtaagcggag
     1501 cacctgactt tttttcgcag gaaacattaa atcttcttag cctacaaatt cccggatagc
     1561 ttccagtgtt ttttcgcgtt cctctaaaaa acacatgtga ccggaattct ccaatagcaa
     1621 acctgaaaca gaaggatgca attccatttg ttcaacggat agattggctg caataacgtt
     1681 atcgtacttt cctgcaataa acaacaccgg gaatttcccg gatcgaatta atccggtttt
     1741 atcttcacgg tctttcattc cccttaaaca cgctgaagca ccatacactg atgtctttaa
     1801 ggcttcggta cgaatcattt caatttcttc agaaaaccgc tcaacattaa atggagcaaa
     1861 taaatttgga attgcttccc gaataaaaac tgcaggactc atttcaagaa cacgaattgc
     1921 acgcaaacga tctgccttct tctcttccgt gtcgggaaat gcagtagaat taatcaatcc
     1981 gattccttca caatagtctg cataccgctc cgcaaaggac aatgcagcat atccacccat
     2041 tgaatgcccc aggacctttg cttttttttc tttttcctgt ttcaatacgg ccacaacaac
     2101 atccgccatc agcggcatcg tatgcacctc cgaaaacaca tccgtcgatc cgtgaccagg
     2161 cagatcaatt gaaattattt tgaagcgttg agataattgc tttgaaaaat agtgccagat
     2221 aaaggaagac tccagaaaac cgtgcagcaa tacaactacc ggtcctgctc cctctgtgta
     2281 ataccgaatt ttctttccgt tataggtgca aaaatgtggt gtcatttctt tttcatcaag
     2341 gcctcaatct catctgcctc aacaggaata gccgccatca gatcacgtgg gtcgctctct
     2401 gttaccatta cattattctc cagacgaatt cctaaatttt cttcgcgaat atatattccc
     2461 ggctcaacag taaacaccat tccgggctga ataggtttgt gccaaagtcc tacatcgtgc
     2521 acatccaagc caagaaaatg tgaagtacca tgcatgaaat atttcttgta tgcaggcatt
     2581 gaaggatcct gatttttaac atcagtttca ctgagcagtc caagtccggt taattccttc
     2641 tccattattt ttccaacttc cttgtgataa tccgcaagca tggtacctgg tctcaacatc
     2701 ttcatcgatt cgcgcattac acgcaataca gcattgtaaa catccttctg acgctttgta
     2761 aattttccgt tgacaggaac acaacgcgtc atatccgcat tgtaattggc atattctgca
     2821 ccaacatcca gcaggatcac atcaccgtcc ttgcattctg cattgttgtc gatataatgc
     2881 aaaacacatg cgttgaatcc actggcaata ataggtgtat aggcaaatcc ccgcgaacgg
     2941 ttgcgaacaa actcatgaat gatttctgct tcgatctcgt actccattac acccggtttg
     3001 atgtaaccaa gtattctgcg gaatgctttt tcggtaatat cgatagccgt ttgcatgagt
     3061 tcgatttcca atccggattt tacgctgcgg atctggtgca tcaatggagc aactcgttca
     3121 agtgaatgca gtgggaaatg ttcttttatc cattgaataa accgggcgtc gcgtgtttgg
     3181 gtttcaatat gtgcacgcaa atgttcgtta gtattcagac aaattgtttc agcttcaaat
     3241 gccagcattt taaatacccg atcgaactga gacaaccaat acactgtttt tatccctgaa
     3301 gtttcataag cactacgctt gctgtgttta gctccttccc aaatggcaat ttcttcgttg
     3361 gtctcgcgta aaaacaaaac ttcgcgatga gcaggatcgt gcgcaaaggg aaacaaaaca
     3421 agaatacttt cttcctgatc aatacccgac aagtaaaaca gatcattgtt ctgacgaaac
     3481 gtcatgtgac catccgcatt ggtaggcaac agatcgttcg agttaaacac cgcaattgat
     3541 cccggcttaa gcatagctgc aaaacgcttg cggttatcaa cgaataattt ggcgttaagt
     3601 ggaaggtatt tcatgcggca gtgttttcct caaattgaca aaatatcggg gaagaatgca
     3661 aaccaaaatt tcaggaaaca aaaaaagcgg ttcagttaat tgaaccgctt taaaatttat
     3721 tggtgaaatg tgattattta ccgggattcc aaaaatcttc cacaaacttg gtggtaaaat
     3781 ttccggcgcg gaaatcttcg tttttcagca atgcctgatg aaagggaatg gatgttttta
     3841 ttccttcgat aatgtactcg tcaagcgctc tttgcatctt ggtaatcgcc tcttcgcgcg
     3901 tacgtgcaac ggtgatcagt tttccaacca tcgaatcgta gttcggggga atcacataac
     3961 ctgcgtatac atgcgtgtcg atacgaatac cgtgtccgcc cggtgaatgg taattggtga
     4021 ttttaccggg actgggtgca aaattgcgga atggatcttc tgcattgata cgacactgaa
     4081 tagcgtgcaa ttgcggctcg taattttttc cggagatcgc aacaccggct gcaattttga
     4141 tttgctcttt taccaggtca taatcgatta cttcttctgt aatggtatgt tccacctgaa
     4201 tacgggtgtt catttccatg aagtaaaaat tgcggtgttt gtcaaccaga aactcgattg
     4261 ttccaacacc ttcgtatttg atggctttaa ctgctttgat tgcagcatca cccatttttt
     4321 tacgcaactc aggagtcatg aaaggagaag gcgtttcttc aaccagtttt tggtgacgac
     4381 gctgaatgga gcaatcgcgc tcggagaggt gacaggcatt accaaattga tcaccggcaa
     4441 cctgaatttc gatgtggcgt ggttcttcga tgaacttctc catgtacaca ccgtcgttac
     4501 caaatgcagc acctgcttcc tgacgtgcgc tatcccatgc agcttccaga tcttcttctt
     4561 tccataccac acgcattcct ttaccaccac caccggcagt agctttaaga atgatcgggt
     4621 attttatttt agcagcgatc tttttcgctt cggccatgtc ggcaagcaat ccatcagaac
     4681 ccggaataac aggtactcct gctttcttca tggtgtcttt ggcacttgcc ttatcgccca
     4741 ttgaatcgat ctgataaggt gttgcaccaa tgaacttgat gttgtgatcc tgacaaatct
     4801 tcgaaaattt tgaattctca gagaggaaac cgtaacccgg atgaatggca tctgcattgg
     4861 tgatttctgc agcagcgatg atacgcgaca tgttcaggta agaatccttg ctgggtgggg
     4921 gaccaataca aactgcttca tctgcgaagc gaacatgcag ggaatcttta tcggcggtag
     4981 aataaacggc taccgtttgg atgcccattt ccttgcaggt acgaataacc cgcaacgcaa
     5041 tttctccgcg gtttgcgatg agaattttat gaaacatttt atctccttct tctccggttt
     5101 tctttttgcc ggatgatttt ttgaaaatag ccataacgaa atgcttaaat gaaaacagag
     5161 ccgtatcctc cgatacggct cgtaactatt tcttttgcga tcaggcaggt tctaccagga
     5221 acaatggctg atcgtattcc accggcttgg cgtcatccat cagcaccttc acaatcttac
     5281 cggaaatttc cgcttcgatt tcgttgaaga gtttcatggc ttcgatgata cagatcttat
     5341 caccagcttt gatggtgtct cctacattca caaaaacagg tttttcaggt cccggtgagc
     5401 ggtagaaggt tccgatcatt ggtgatttga tggttacata cttcgaatcg tcaccaccgg
     5461 cagcaggagc aacaggtgcc tcagcctgag gtgcagcggt aacaggcgca gcagcaatca
     5521 tctgctgggc aggtgcggct acttgtgtta caacaggccc ggcagcttgt acaattgcag
     5581 cttttgcacg gatcacgatc ttaaaatcct ttttttcaat ctcaacttcg gtaactcccg
     5641 atttagagac gaatttgatc aggtcctgga tctcattcag attcatacgc ttgattttgg
     5701 ttttgctttc agttggaatt atatgcccat ttcaaatata gcgctcccca ggtaaatcct
     5761 cctccaaaag cagaaagaat gaggttgtcg cccttgcgca gtttgctttc ccattcccag
     5821 agcaaaaggg gaatggttcc tgcggttgta tttccgaatt tttgaatatt gatcatcacc
     5881 ttggccttat ccagtcccat acgttcagca gtggcgtcga tgatgcgcaa attggcctgg
     5941 tgcggcacca gccaggtaac atcatcggct gtaagcttat tcttttccat gatctcggca
     6001 gaaacatcgg ccatgttggt cacggcaaat ttgaaaacgg ccttaccttc ctggtaaacg
     6061 taatgctgtt tgttatccac ggtttcgtga gaagcgggtt ttaaagaacc ccctgcaggc
     6121 tggcataaat attttcttcc ggatccgtcg gctcgcaata tgaaatcctt tactccatag
     6181 ccttctgtat cgggctcaag caatactgct cctcctccat caccaaaaat tacgcaagtg
     6241 gtacgatcgg tataatcgag aatggatgac atcatatccg ctcccacaac aaccactttt
     6301 ttgtaacgac cggtttcaat gaattgtgcg cctgtagcca aggcataaat aaatcctgaa
     6361 caggcagcag agagatcgta accccaggca tttttggcgc ccagtttatc gcaaaccaca
     6421 ttactggtgg atgggaaatt gtagtcgctg gtaactgttc ccactattag caaatcaatg
     6481 tcttccggac caattccacg tttactgagt aattgcttga ctgcttcaac gcaaatatca
     6541 gacaatgcct ttccttcgcc cttgagaatg cgtcgctcgg aaataccggt tctcgatttg
     6601 atccattcgt cgttggtatc caccattgtg gaaagctcct cattggtgag aacgtactcg
     6661 gggacatatc ctcctattgc cgtaattgct gctgtaatgc gactcatttt gcaaacactc
     6721 ctttgatttt ttcgggtatg ccggctttca ccacatccct gctcaaaagt atcatatttt
     6781 taatcgctgt agcattggaa atgccatgac caattatcac gttggaattc acgcccaata
     6841 cgggagttcc gccataattc tcatagttga aacgatcgaa atattcgtct ctgatccccc
     6901 gtttacgcat cagccagtat aacccttcag cttctttcaa aacaacatt
//
LOCUS       DAOMYG010000124         6946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3678_length_6946_cov_12.336526, whole genome shotgun sequence.
ACCESSION   DAOMYG010000124 DAOMYG010000000
VERSION     DAOMYG010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6946
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3678_length_6946_cov_12.336526"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PK610_05425"
     CDS             complement(<1..505)
                     /locus_tag="PK610_05425"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRJ35405.1"
                     /translation="MRKTLYILLLLAFACSGEHKHDHGHEHDSTGHEHGEHEHGHHHG
                     DANAYMNESEFDELVQRFEDPEREKWQKPEVVIAFIGDLKGKTLVDIGAGTGYFAFKL
                     ADGAEKIIAADADERFIDYIENKNKDAKKNNLITRKAEYASAPLEANEADVVLLVNVY
                     HHIEKRND"
     gene            complement(729..2573)
                     /gene="glmS"
                     /locus_tag="PK610_05430"
     CDS             complement(729..2573)
                     /gene="glmS"
                     /locus_tag="PK610_05430"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRJ35406.1"
                     /translation="MCGIVGYIGTKDAYPILIKGLKRLEYRGYDSAGIALVEGGHLNV
                     YKKKGKVAELEAFATDKNIKGTIGIGHTRWATHGQPNDENAHPHFSGDSNLAIIHNGI
                     IENYATIKEELSKRGHVFTSETDTEVLIHFIEEIRNKEKVDLFEAVRIALNEVHGAYA
                     IVVIDKSNPDRLIAAKKSSPLVIGIGEEGEFFLASDATPIVEYTKNVVYLEDEEIAVI
                     DRKEGLKLKNIKNQDKRLSIQELEMQLEALEKGGYEHFMLKEIYEQPRSIRDCMRGRI
                     SATQGWVQLGGIKDFEQKLTSTKRIIMVACGTSWHAGLVGEYLFEELARIPVEVEYAS
                     EFRYRNPIVDENDVVIAISQSGETADTLAAIQLAKSKGATIIGICNVVGSTIARSTDA
                     GSYTHAGPEIGVASTKAFTAQVTVLTLMALMIGQKRGTIPANRFNRLLIELDSIPEKV
                     EEVLKSNSQIKYIADIYKDARNALYLGRGNSFPVALEGALKLKEISYIHAEGYPAAEM
                     KHGPIALIDEEMPVFVIATQGPSYEKVVSNIQEVKARKGKIIAIVTKGDTVVKGLADH
                     IIEIPDTDDILVPLLSVIPLQLLSYHIAVMRGCNVDQPRNLAKSVTVE"
     gene            complement(2577..3917)
                     /locus_tag="PK610_05435"
     CDS             complement(2577..3917)
                     /locus_tag="PK610_05435"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HRJ35407.1"
                     /translation="MKTTSFSKAQRSFMAAVFFVALSLGACRKSDADLGATVQPADDI
                     LNGTVMDTLTLRTFTRVSDSLRTDELANNLIGSINDPVFGKAKAGIFTQIRLAANAPD
                     FTPGDIIIDSLVLSLVYQSYYGKLDAQQFHVYEITDTLYQDSAYYSTTVKQTDPTDLV
                     VGGYSIQTPNTLSWFHDPIIGDSSKPQLRLRLDNSLAQRFANESGNATLADNDNFTRW
                     FKGLHVTVDNSAQAVGNGALMYIDMLDPNTKVTMYYRRISTGDTVRFSFLINDRCARY
                     TRYTHDYSGTEVALQLADSTLGNNYFYLQAGAGLQAEIHIPHLMELNNNGNIIVNLAE
                     LILPVQYFNLDPLTPAPQIILFGINDEGESYTVADLFLGDLIFGGYYDASRKVYRFSI
                     TRHIQRILNGTHPNKGLRLAVVASSISGNRSILSGAGADNRDKPSLKIIYTKYQ"
     gene            complement(3930..4751)
                     /locus_tag="PK610_05440"
     CDS             complement(3930..4751)
                     /locus_tag="PK610_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRJ35408.1"
                     /translation="MEKLRVLYVSQEITPFLPETTLSTISRKLPQGVQEIDKEVRVFM
                     PRYGCINERRHQLHEVIRLSGMNIIINDNDHPLIIKVASIPSARMQVYFIDNEDYFKR
                     KNVVSDDSGKFFDDNDERSIFFCRGVLETVKKLGWVPDVIHCHGWMTALMPAYVKRFY
                     GDDPHFADTKVVYSVYDNGFEGSLDKKLAGKMKSDGFTDGDVTHLKAPDFVNLTKMAV
                     QHADGVILGSEKVDAKINSFLKESGKPVLQYQEEDRYIPIYNEFYDKVAEALLVD"
     gene            4876..5739
                     /gene="panC"
                     /locus_tag="PK610_05445"
     CDS             4876..5739
                     /gene="panC"
                     /locus_tag="PK610_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRJ35409.1"
                     /translation="MKTLAFPHELTSLSDTLKREGKSLGFVPTMGALHEGHLSLVREA
                     KSKNDVVVASIFVNPTQFNDPKDFERYPRVVEEDQRMLESAGCDVLFLPSVKDMYPDD
                     ASQQAVSFGFLETVLEGAHRPGHFSGVGMVVKRLFEIVKPSTAYFGSKDYQQVMVIRS
                     LVEQFSIPVEIIAMPTIRESDGLAMSSRNRLLSAEERTIALALSKALFTAKENYPSQS
                     IPELLQHAIATLSRPDIRLEYFVICDGDTLREITDKSQTKNPVALVAAHVGKVRLIDN
                     LGLGRAVNSEQ"
     gene            6362..>6946
                     /locus_tag="PK610_05450"
     CDS             6362..>6946
                     /locus_tag="PK610_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ35410.1"
                     /translation="MKIEQIYTGCLAEAAYYIESNGEAAIIDPLREVEPYIKKAKENN
                     ATIKYVFETHFHADFVSGHVDLAAKTGAAIVYGPTKMETGFKAHIAHDGELFRIGNIS
                     IELIHTPGHTMESSCYLLRDENGKETAIFTGDTLFIGDVGRPDLAQHVISDLTEDKLA
                     GHLFDSLRNKIMPLNDDIVVYPGHGAGSACGKNMS"
BASE COUNT         1763 a         1655 c         1608 g         1920 t
ORIGIN      
        1 aatcgtttcg tttttcaatg tgatggtaca cattcaccaa tagcaccaca tcggcttcgt
       61 tcgcttcgag aggagcagaa gcatattcag ctttgcgggt gatgagattg ttttttttcg
      121 cgtccttgtt tttgttttcg atgtagtcta taaaacgttc atcagcatcg gcagcaataa
      181 ttttttccgc accatcggcc agcttaaatg caaagtaacc ggtacccgca ccaatgtcca
      241 ccagcgtttt ccctttcaga tcgccaataa acgcaatcac cacctccggt ttctgccact
      301 tctcccgctc cggatcctca aagcgctgca ccagttcatc gaattcactc tcgttcatgt
      361 acgcattcgc atccccatga tgatgcccgt gctcatgttc cccatgctca tgcccggtac
      421 tgtcatgctc atgcccatga tcgtgcttat gctcacccga acaggcgaaa gcgaggagga
      481 gaaggatgta tagtgtttta cgcatgggag gggggtaatt ggttaatctt ccttcggtaa
      541 accttcggcg gacaagttgg gtaatcctcc ttcgctaaac cttcagcgga caagttggaa
      601 tattggtaat aatacggatt tcatcggcaa cacctattca ttcgcaaact agcacattcg
      661 cacattctca cattctcaca ttctctcatt cacccattcg cacactagca cattcgcaca
      721 ttatccttct actccaccgt caccgatttc gccaggttac gcggctgatc cacgttgcac
      781 ccacgcatta cggcgatgtg gtacgataac aactgcaaag gaatcaccga gagtagcgga
      841 acaagaatat catccgtatc cggaatctca atgatatgat cggccagtcc ttttaccacg
      901 gtatcacctt tggtaacaat ggcaatgatc tttcctttgc gggcttttac ttcctgaata
      961 ttgctcacca ctttttcgta cgatggtccc tgcgtggcga tcacaaacac gggcatctct
     1021 tcgtcgatca gtgcgatcgg accgtgtttc atttctgcag caggatagcc ttcggcgtgg
     1081 atgtaggaaa tttctttcag cttcagcgcg ccttccaatg caaccggaaa actgtttccg
     1141 cgaccaaggt aaagtgcgtt gcgtgcgtct ttgtagatgt cggcaatgta tttgatctgc
     1201 gaattgctct tgagtacttc ttccaccttt tcgggaatgg aatcgagttc gatgagtaaa
     1261 cggttaaagc ggttggcagg aatggtgccg cgtttttgtc cgatcatcag cgccataagg
     1321 gtaagcactg ttacttgtgc cgtaaacgcc ttagtagagg ccacaccaat ttccggaccg
     1381 gcatgggtgt acgatccggc atcggttgag cgtgcaatgg ttgaacccac cacattacaa
     1441 ataccgataa tggttgcacc tttcgatttg gcaagctgaa tggcagccag cgtatcggcc
     1501 gtttctcccg attgtgagat ggcaatcact acatcgtttt catcaacgat cggattgcgg
     1561 taacggaatt cggatgcgta ttctacttct acgggaatgc gcgccagctc ttcgaaaagg
     1621 tattcgccca ccagtcctgc atgccatgat gttccgcagg ctaccatgat gatgcgtttg
     1681 gtggaagtaa gtttctgttc gaaatctttt attcctccca gctgcaccca gccttgcgtg
     1741 gcactgatcc ttccgcgcat acaatcgcga atggaacggg gctgctcgta aatctccttg
     1801 agcatgaaat gttcgtagcc gcctttttcc agcgcttcga gttgcatttc cagttcctgt
     1861 atcgagagac gtttgtcctg gttcttgatg ttcttcagtt tcaatccttc cttgcgatcg
     1921 atcacggcaa tttcttcgtc ctcgaggtac accacatttt tggtgtattc tacaatgggg
     1981 gtggcatcgg atgcgagaaa aaattcgcct tcttctccaa taccgatcac caacggagat
     2041 gatttttttg cagcgatcag tcggtcggga ttgctcttgt cgatcaccac aatggcatac
     2101 gcgccgtgta cttcgttcaa tgcaatacgc accgcttcaa agagatctac tttttccttg
     2161 ttgcggatct cttcaatgaa atggatgagg acttctgtat cggtttcaga ggtaaaaacg
     2221 tgaccgcgtt tggataattc ttccttaatg gtggcgtagt tctctatgat tccgttgtga
     2281 ataatagcga gattactatc acccgaaaaa tgcgggtgtg cgttttcgtc gttcggctga
     2341 ccatgcgttg cccagcgggt gtgaccaata ccgattgtac ctttgatgtt tttatcggtg
     2401 gcaaaagctt cgagttctgc aacctttcct tttttcttgt acacgttcaa atgaccacct
     2461 tccaccaagg caatacctgc cgaatcgtat ccgcggtatt cgaggcgttt tagtccttta
     2521 atgaggatag gataagcgtc cttagttcct atgtatccaa cgattccgca catggctcac
     2581 tggtatttgg tgtaaataat ttttagactt ggcttgtcgc gattgtcggc gcccgcacca
     2641 cttaatatac ttctgttccc ggaaatggaa gaggcaacaa cagctaaacg caatccttta
     2701 ttcgggtgtg ttccgttcag gatgcgctgg atatgtcgtg taatggaaaa gcgatagact
     2761 ttacgactgg catcatagta accaccaaaa atgagatcgc ccaaaaagag gtctgcaaca
     2821 gtgtagcttt cgccttcgtc gtttattcca aaaagaatga tttgcggcgc aggagtgagc
     2881 ggatcgaggt tgaagtattg tacgggtaag atcagttctg caagattaac gatgatgttg
     2941 ccattgttgt tgagctccat caaatgcgga atatgaattt ccgcttgtaa tcctgctccc
     3001 gcctgcaggt aaaaataatt atttcccagt gtgctgtctg ctaattgcag tgctacttcg
     3061 gttccggaat aatcgtgtgt gtacctggtg taacgtgcgc aacgatcgtt gatgagaaaa
     3121 gaaaaacgaa cggtatctcc ggtgctgatg cgacggtaat acatcgtcac ttttgtattg
     3181 ggatcgagca tatcgatata catcaatgct ccgttgccaa cagcttgtgc gctgttatct
     3241 actgttacat gcaatccttt gaaccaacgt gtgaagttgt cattgtcggc cagtgttgca
     3301 tttccgcttt cattggcaaa acgttgtgca agcgaattat ccaaacgcag gcgcaattgt
     3361 ggtttggaag aatcgccgat gatggggtcg tgaaaccagg aaagcgtatt gggtgtttga
     3421 atggaataac cacccacaac aagatcggtg ggatcggttt gtttaacggt ggtggaataa
     3481 taggccgaat cctgatagag tgtatcggtt atttcgtaaa catgaaattg ttgtgcatca
     3541 agtttgccgt agtacgattg gtaaacgagc gatagcacca gcgaatcgat aataatatcg
     3601 cccggagtga aatcgggtgc gttggcggcc agacggatct gcgtgaaaat accggctttc
     3661 gcttttccga aaacaggatc gttgatgctt ccgatcaggt tattggccag ctcatcggta
     3721 cgcaacgaat ccgatacccg cgtaaaagtg cgcagggtaa gggtgtccat tactgttccg
     3781 ttgaggatat cgtctgcggg ttgaacagtg gctccgaggt cggcatctga cttgcggcac
     3841 gcacccagac taagggcaac aaaaaagaca gcggccataa atgaccgctg agctttagag
     3901 aatgatgtag ttttcaattt cattgtggtt tagtccacca gaagtgcttc ggcgactttg
     3961 tcgtagaatt cgttgtagat ggggatgtag cgatcttctt cctggtattg aagaacgggt
     4021 ttgccgcttt ctttgaggaa agaattgatt ttcgcatcca ctttttctga tccgagaatg
     4081 acaccatcgg catgctgaac agccattttg gtgaggttaa caaaatcggg agcttttagg
     4141 tgggtaacat cgccgtcggt aaatccgtcg gacttcattt tcccggccag ctttttatca
     4201 agtgaacctt caaaaccatt atcgtaaaca gaataaacca ccttggtatc tgcaaaatgc
     4261 ggatcgtcgc cataaaaacg tttaacgtaa gcaggcatca gagctgtcat ccagccgtgg
     4321 cagtgaatta catcgggaac ccaacccagt ttctttacgg tttcaagaac accgcggcag
     4381 aaaaagatcg aacgctcgtc gttatcgtca aagaactttc cggaatcatc actaacaacg
     4441 ttctttcttt tgaaatagtc ttcgttatcg ataaaataaa cctgcattct tgcagaaggg
     4501 atagaggcca ccttaatgat gagcgggtga tcgttgtcgt tgatgatgat gttcatccct
     4561 gagagacgaa tcacttcatg cagttggtga cgacgctcat tgatgcatcc gtaacgtggc
     4621 ataaagacgc gtacttcttt gtcgatttcc tgtacgccct gaggcaattt gcgtgaaatg
     4681 gtagagagag tggtttccgg aagaaaagga gtaatctcct gggaaacata aagtacacga
     4741 agtttttcca taatggggtt ttacgttcct gcagaattgc attgcgggta caaaaatata
     4801 gaaaaaaaac ggcactttca acaaatcgac ttagatttgc ccgctccgct acccacccac
     4861 ctccccctca agcccttgaa aacattagcg tttccgcacg aattgacctc tctttccgac
     4921 accctgaaaa gggagggtaa gagcctcggt tttgtgccca ccatgggcgc tttgcatgaa
     4981 ggacacctct cccttgtccg cgaggccaag tccaaaaacg acgttgtggt ggccagtatt
     5041 tttgtgaatc cgactcagtt taatgacccc aaagatttcg aacgctatcc ccgcgtagtg
     5101 gaagaagatc aacgcatgtt ggaatcagct ggatgcgatg tgctttttct tccttcggta
     5161 aaggatatgt atcccgacga tgcttcacaa caagctgttt cctttggctt tctggaaact
     5221 gtattggaag gcgctcaccg tcccggccat ttttccggtg tgggaatggt agtaaagcgt
     5281 ctgtttgaaa tcgtaaagcc atcaactgcc tatttcggga gcaaagacta ccagcaagta
     5341 atggtgatcc gttcgctggt ggagcagttt tccattcctg tggagatcat tgccatgccc
     5401 accatccgcg aaagcgacgg actcgccatg agttcgcgca accgcttgtt aagtgctgaa
     5461 gaacggacaa tagcacttgc acttagtaaa gccttgttta ctgctaagga aaattaccca
     5521 tcacaaagca ttcccgaact cctgcagcat gccatcgcaa cactcagccg ccccgacatc
     5581 cgcctcgaat atttcgtcat ctgcgacggc gacaccctcc gagagattac cgataaatcg
     5641 caaaccaaaa accccgtggc actcgtagcc gcacacgtag gcaaggtgag gttgattgat
     5701 aatttggggt tgggcagggc tgtgaatagt gaacagtaaa cggttgtcaa tgcctgaata
     5761 aggttgacgg cttaaggtta atttacttca ttcccaaata cacaattaga cacctatttc
     5821 caagtgttac ccctttgcgc agcgccgaag gtgactttgc tctccttgaa aaaatccgaa
     5881 ttggtattct caaaacaaat tcagaagagg ataattattc aagcagatca caaacagatt
     5941 tctttgcggt aagtgtttta gtattctacg tgctattttg tcgcctatgg agtaaggttc
     6001 atgcgcttcc cgtgtctttg cgatcaaatc ggagtgtaat attgccttat cagtaacatt
     6061 tgttactcac cactattcaa ccatccacca aatgtgacat ttgtcattct gcatcatttt
     6121 ctacctgccg atctttgtat tgtaaaattt ctgccatgta cacaacagtt tttgttattg
     6181 tcctgctcca cctgattgcc gggtttggat acttgtttta taaactggaa tgggcaaaat
     6241 tgaacaagaa ggaagatccg gaaaaccact aatatgaaca gcacttgcag cacacgttgt
     6301 ccctgatgga aagtgacaat ttctcactcc accatttcgt tcaattaaaa aaacagtcac
     6361 tatgaaaatc gaacaaatct ataccggatg tctggccgaa gcggcgtatt acatcgaaag
     6421 caatggcgaa gcagccatca ttgatccgct tcgcgaagta gagccttaca tcaaaaaggc
     6481 caaagaaaac aatgcaacaa tcaaatacgt tttcgaaacg catttccatg ccgatttcgt
     6541 ttcgggtcac gtcgatctcg ctgccaaaac aggtgcagct attgtttatg gtcctaccaa
     6601 aatggaaacc ggatttaaag cacacattgc gcacgacgga gaattgttcc gcatcggaaa
     6661 catctccata gagctcatcc acacgcccgg acataccatg gaaagctcct gttacctgct
     6721 ccgcgatgag aacggaaagg aaacagccat tttcaccggc gatacgctct ttatcggcga
     6781 tgtaggtcgc cccgatctgg cacagcacgt gatttcggat ctgaccgaag ataaactggc
     6841 aggacatttg ttcgattcgt tgcgtaacaa gatcatgccg ctcaatgatg atatcgttgt
     6901 gtatcccggt catggtgccg gcagtgcatg cggaaagaac atgagt
//
LOCUS       DAOMYG010000125         6917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3698_length_6917_cov_10.975517, whole genome shotgun sequence.
ACCESSION   DAOMYG010000125 DAOMYG010000000
VERSION     DAOMYG010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3698_length_6917_cov_10.975517"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..431
                     /locus_tag="PK610_05455"
     CDS             93..431
                     /locus_tag="PK610_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35411.1"
                     /translation="MDSPKNLDHYSEAKFLFDSGYDSKAILARLLEKGIDDNQAQETL
                     NKLKKEHLEKRRKRGLVFIAIGGICLFSGFLLSAILFHSNSSPAIPLYGMTSVGIVLL
                     LVGMADCLGL"
     gene            complement(489..6707)
                     /locus_tag="PK610_05460"
     CDS             complement(489..6707)
                     /locus_tag="PK610_05460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ35412.1"
                     /translation="MKRLLLFSLGVITLAGNLFAQAPGCPNIVAPADIVLPCTQACTT
                     LTATPFHVGATTTYTVGATSATPVIPYNQAGGTAIVVGGDDVYSGVINLPFPFCYYGN
                     TYTQCVVGSNGVLTFNPGLANGYCPWFFSVDCPNAALPLNSIFGIFMDEWPGQCGQVK
                     FHLIGTAPCRMLVVNYDDVCQYSCLTSARTNSQIVLYETTNVIEVYTNKKQSCAWNGG
                     RAIIGIQNATGAVGMAAPGRNTTNAIWTPPATGEGWRFTPNGAPIYNVTWWQGATQIG
                     AGNSINVCPTASPTTYTARATYTRCDGLVINVNDDVVVTLSALTAPAITTVAETCNNY
                     NNGSVTINNPVGAGPYTVTITGPANPPPVVEPNTAAGVATFNNLPDGTYNYLVTSANG
                     CTVNGTFSIAAGPPCCNVTANGTNALCNGGSTGTASANPVGQAPFNYSWNSAPVQNSQ
                     TATTLPAGTYTVTMTDASGCTATASYIVTQPTALTGTLSQVNVTCNGLCNGSITVNGA
                     GGGTGALQYSINGGPFQASNTFNGLCAGAYTVIVRDANNCTRTLNATITQPAVLTLAA
                     GSLVPATCGNPNGSVVLAAGGGTVNYQFNIGGPNQASGTFTGLAPNTYTATVTDQNGC
                     TATTSFTIANQPGPVASIGTQSNVSCAGGVNGSVVINAAGGTGALTYDLNPGPPPQAS
                     NTFTGLAAGAYSVIVADANGCSNTVNFTITQPTQLTFTSVSQNISCNGVCDGQITVTA
                     NNATPPYQYSSNGGLTFQASNVLSGICAGTINVVVRDANGCLANANVILTQPAALTAT
                     YTPTNPICQGICNGQIAVATSAGGTAPYQFSVDGGALQAGTSFTGLCSGNHTMLIRDA
                     NGCQVTAVVNLVDPPGYTVNTISSTSSNCGFNDGAFTVQATGGNAPYTYNNITVGVSN
                     ATGVFPDLVAGAYQVVVTDALGCQEIQFVGVNDIQMNGLLLGSTNPTCPGACNGTVST
                     IATSGFGTISYDLDNGMQSAFGSGDFSGICDGSHAITMTDQGLCIFVVPFTLVAPPSI
                     QFTTAVTNTLCNGGSDGIITFNAPSGGTPPYQYSVDNGVTFQAGSVFTGLAAGTYNVV
                     VRDGNNCTQNGTATIGQPNPLNISSSHTDLTCFGNNSGTLLIVGTGGTGTIQYSNNGG
                     TTFGATFSFFGLAAGVYNIAIRDANGCIVNSTATIVEPPLLTATYATTPALCNTSCDG
                     TIQVNANGGTAPYLYSSNNGVVFQTASLLENLCAGSYQVQVVDDNGCLVGSNQLITEP
                     TAVAFTTVITPSTCGNPNGSIVINANGGNGTFLYSIDNGITFQVPNTFNGLAAGIYNI
                     VVEDGNGCQENATADVQNQSSPVINALFATDLLCNAVCNGQLDVTATGGTGALNYDIG
                     GATQPGGIFASLCAGNYTVTVTDANGCTASQNASITEPPVLTLASTPTDLNCFGDNSG
                     EISLVAAGGVTPYLYSYDNGVTQSSFSTQTGFSAGNYTTIVTDANGCQTTSPVVVAQP
                     TQMTITNQNTVNASCFGVCDGEVSITISGGTTAGFYTHQWANGIGGPNQNTVLGLCAG
                     SYSVTVVDDNACSIDALFNVTQPVAVVVSSFTATNPTCAGSCDGQLTAIGVNVVDYSI
                     DGVNFQVSPTFTGLCSGVYTLTGRDINGCTVSLTATLTEPTPVVLDPIAPITICAGFT
                     GVLSSQANGGTPPYNYIWNTGDDAQYYTVTSLVNTTFTVTAFDLNGCPSAPQTGDVSV
                     IPSFVPTTTGDVNVCPGSPASITASATGGMPGYIFTWLYENDTLVDSPTLTFVPSAPG
                     TVMLVAEDGCPDLDTIYINVGFHVTPQPTATVTPSSGCAPLTVDFVNTTPASQVGTDC
                     SWSFGDGGANPGCGAQSHVYTTPGCYDVTLTVVSPEGCVGSQTFDDIVCVYGDPIADF
                     TYNPSTPTIINSSINFVDASTNGATYQWDFAGMGTSTQQNPTFNFVNVDTGFYQVCLT
                     VTSPQGCVDDTCKMVYIFDEFLVYVPNAFTPDGDGINDVFMPVLTGLNPEHFELLIFN
                     RWGEVIYQTNNPTKGWDGRSKGIMSKQDVYVWKIRAKDALLDIDKEFVGHVTLLIE"
BASE COUNT         2085 a         1496 c         1444 g         1892 t
ORIGIN      
        1 aagaccacaa accagccgga caacagggaa acctgacaac tatcatttta ttgaacggca
       61 caaaatcact aaattctagc acatgaaaat ctatggactc accaaagaac ctcgaccatt
      121 attcagaagc aaaattcctt tttgattccg gttacgactc gaaagctatt ctggcacgtc
      181 tgctcgaaaa gggtattgac gacaaccagg cacaggaaac gctgaataag ctaaaaaaag
      241 aacacctcga aaaaagaaga aaaagaggtt tggtttttat cgcgattggc ggaatttgcc
      301 tgttctccgg attcctttta tctgcgatat tgtttcattc caattcatcg cctgcaattc
      361 ctttatatgg catgacctct gttggtatag ttttattact tgtaggcatg gctgattgtc
      421 tcggattata aatcaaaaaa aaaccactgc agattgcagt ggtttttttt catcaatgat
      481 ttctattatc attcaattaa cagagtaacg tgtcccacga attctttatc aatatccaat
      541 aaagcatctt tcgcgcggat cttccacacg tatacgtcct gtttagacat tattccttta
      601 cttcttccat cccacccttt tgttggatta ttggtttgat aaatcacttc tccccaacga
      661 ttgaaaataa gtaattcgaa atgttcagga tttaatccgg taagaaccgg cataaacaca
      721 tcgtttattc catcaccatc aggagtaaat gcgttaggaa catacaccag gaattcatca
      781 aaaatgtaaa ccattttgca agtgtcatcc acacatccct gaggcgatgt aacggtcaga
      841 caaacctgat aaaatccggt atcaacattt acaaaattga atgtcggatt ttgttgtgtt
      901 gaagttccca tacccgcaaa atcccactgg tacgttgctc cgtttgttga agcatccaca
      961 aagtttattg aagaattgat aatggttggc gtggatgggt tgtaagtgaa atctgctatt
     1021 ggatcaccat aaacacatac aatatcatca aacgtttgag atcccacaca gccttccggt
     1081 gaaacaacag taagtgtaac atcataacaa cccggagtag tataaacatg agactgcgca
     1141 ccgcaaccgg gattagcacc gccatcaccg aatgaccatg aacaatctgt tccaacctga
     1201 gaagcaggag ttgtatttac aaaatcaacc gttaatggtg cacatcctga agatggagta
     1261 acagttgctg ttggttgagg agtaacatgg aaacctacgt taatataaat tgtatcaaga
     1321 tcaggacaac catcttcggc aaccagcata actgtacctg gtgctgaagg aacaaatgta
     1381 agagtaggac tatcaaccaa tgtatcgttt tcatataacc aggtgaaaat atatccaggc
     1441 attcctcctg ttgcacttgc agtaattgaa gcaggtgaac ccggacaaac attcacatca
     1501 ccagtagttg tgggaacaaa gcttggtatt acactcacat cacctgtttg aggtgcagaa
     1561 ggacagccgt tcaaatcgaa tgccgtaaca gtgaacgtag tatttacaag agacgtaacg
     1621 gtatagtatt gtgcatcatc gccggtgttc cagatatagt tgtaaggtgg tgttcctccg
     1681 tttgcctgtg aagaaagcac accggtaaat ccggcacaaa ttgtaattgg agcgattgga
     1741 tcaagtacta caggagtcgg ttctgttaag gttgcagtta atgataccgt acaaccatta
     1801 atatctcttc ccgtaagtgt ataaacacct gaacacaatc cggtgaaagt cggagaaacc
     1861 tggaagttca ctccatcaat actgtagtca acaacattca caccgattgc tgttagctga
     1921 ccatcgcagg aacctgcaca ggttggattg gttgcagtaa aactacttac aacaactgca
     1981 accggctgag tcacgttaaa cagcgcatca attgaacatg cattatcatc aacaacagtt
     2041 acagaatagg aaccggcaca taatcctaaa acagtattct ggtttggacc accgattccg
     2101 tttgcccact gatgcgtata aaaacctgct gttgttccac cggaaatggt gatactaact
     2161 tctccgtcgc aaactccaaa acaagaagca ttaacagtat tctgattagt gattgtcatt
     2221 tgagtgggct gcgcaacaac cacaggtgag gtagtctgac aaccgtttgc atctgtaaca
     2281 atggtagtat agttgcctgc tgaaaatccg gtttgagtag agaaagaaga ttgggttact
     2341 ccattatcat aactgtataa atacggagta acaccaccgg cggctaccaa tgaaatttct
     2401 ccggaattat caccaaagca atttaagtcg gttggcgtag atgcgagtgt aagaacagga
     2461 ggttcagtta ttgatgcatt ctgagaagct gtacatccat ttgcatctgt aacagttact
     2521 gtataatttc ctgcgcaaag tgaagcgaaa atacctcctg gctgtgtggc tcctccaatg
     2581 tcataattta atgcaccagt tccaccggtt gcagttacat cgagttgacc attgcaaact
     2641 gcattgcaaa gtaagtccgt tgcaaaaaga gcgttgatta caggagagga ttggttctgt
     2701 acatctgctg tagcattctc ctgacatcca tttccatctt ctacaacaat attataaata
     2761 cctgctgcaa gtccattaaa ggtatttggc acctggaatg ttattccgtt atcaatgcta
     2821 taaagaaacg ttccgtttcc gccgtttgca ttaatcacaa tcgaaccatt cggatttccg
     2881 caggtagatg gagtaattac agttgtaaat gcaactgcag ttggttctgt tattagttga
     2941 ttcgaaccga caagacatcc attatcatca acaacttgaa cctgataaga acccgcacat
     3001 aagttttcaa gcaatgaagc cgtttgaaaa accacaccat tattggaact gtacaaataa
     3061 ggagcggttc ctccgttagc attcacctga attgttccat cgcaagatgt attacaaagt
     3121 gcaggcgttg tggcataagt tgcagtgagc aatggaggtt caacaattgt tgctgttgaa
     3181 ttcacaatac aaccgtttgc atcccgaata gcaatattgt aaactcctgc agctaatcca
     3241 aagaaagaaa aagttgcacc aaaagttgtt ccgccattat tgctgtattg aattgttcct
     3301 gttccacctg ttccaacaat cagaagtgtt cctgaattgt ttccgaaaca agtgagatca
     3361 gtatggctcg aagaaatatt taatgggttt ggttgaccaa ttgtagctgt gccattttgt
     3421 gtacagttat ttccatcgcg cacaacaacg ttataggtac ctgctgctaa tcctgtaaaa
     3481 acagatccgg cctggaatgt aacaccattg tcaacgctgt attgataggg aggtgttcct
     3541 cctgaaggag cattgaatgt tattattcca tctgacccac cattacataa agtattggtt
     3601 acagcagtgg taaattgaat ggatggtggt gctaccaatg taaatggtac tacaaaaata
     3661 cacaaacctt ggtcggtcat cgtaatggca tgagatccat cacaaattcc tgagaagtca
     3721 cctgaaccaa atgcagattg cattccgtta tccagatcgt atgaaatagt tccgaaacct
     3781 gaagttgcaa ttgttgaaac tgtaccatta catgctcccg gacaggtcgg gtttgttgag
     3841 cctaacaaca atccattcat ttgaatgtcg tttacaccaa caaattgaat ttcctgacaa
     3901 cccaacgcat ccgttactac cacctggtat gcgccggcaa ccagatcagg aaaaacacct
     3961 gttgcattag acacacctac agtaatgtta ttataagtat aaggcgcatt tcctcctgtt
     4021 gcctgaacgg taaacgcacc atcattaaat ccacagttgg atgaagttga acttatagtg
     4081 ttaactgtat atccaggagg atctacaaga tttactactg ctgttacctg acaaccgttc
     4141 gcatctctga ttaacatggt atgattacca ctgcacaatc ctgtaaatga tgttcctgcc
     4201 tgcaatgcac ctccgtcaac agaaaactga tagggagcag taccgccggc acttgtagca
     4261 accgcaatct gaccattaca aattccctga caaattggat ttgttggtgt ataagtagca
     4321 gtaagtgcag cgggctgagt aagtataacg ttagcgtttg caagacatcc attggcatcg
     4381 cgcacaacaa cattaattgt acctgcacat atgccgctaa gaacattcga tgcctggaaa
     4441 gttaaacctc cattgcttga atattgataa ggtggtgtag cattgtttgc tgtaacagtt
     4501 atttgaccgt cgcaaactcc attacacgaa atattttgcg acaccgatgt aaacgtaagc
     4561 tgcgtaggtt gagtaattgt gaagtttaca gtatttgaac atccgtttgc atcggcaaca
     4621 ataacagaat atgcacctgc agctaatcct gtaaaagtat tggatgcctg aggtggaggt
     4681 ccaggattaa gatcataggt aagtgctcct gtaccgcccg cagcgttaat tactacagaa
     4741 ccattaacac caccggcaca actaacattc gattgtgttc caatagatgc taccggtccg
     4801 ggttggtttg caatggtgaa agaagttgtg gcagtacaac cgttctgatc ggttacagtg
     4861 gctgtataag tattgggagc taatccggta aacgttccgc ttgcctggtt cggaccacca
     4921 atattgaatt gataattcac tgttcctcct ccagcggcaa gaacaacaga gccattcgga
     4981 tttccacatg ttgcaggaac caaacttcct gcagccaaag taagaacagc aggttgagtg
     5041 atggttgcat tcaaggtgcg tgtacagttg tttgcatcac gaacaattac agtgtatgct
     5101 ccagcacata agccgttaaa tgtattggac gcctgaaacg gtccgccgtt aatggaatac
     5161 tgcaatgcac ctgtgccacc accggctcca ttcactgtaa tcgaaccatt acataatccg
     5221 ttacatgtaa cgttaacctg agacaatgtt cctgttaatg ctgttggttg agttacaata
     5281 taggatgctg ttgcagtaca acccgaagca tctgtcatgg taacggtata agttcctgca
     5341 ggcaaagtag tagctgtttg cgaattctga acaggcgcac tgttccaact gtaattaaag
     5401 ggtgcttgtc caacaggatt cgcgctggct gttcccgttg atccaccatt acaaagtgca
     5461 tttgttccat ttgcagttac gttacaacaa gggggaccgg cagcaatgga aaaggttccg
     5521 tttactgtac atccgttcgc gctggttaca agataattgt aagtgccatc cggaagatta
     5581 ttaaacgttg ccacaccggc agctgtatta ggttctacta caggaggagg attggccgga
     5641 ccggtgattg taacggtata gggacctgct ccaacgggat tattgatggt aaccgaccca
     5701 ttattataat tgttacaggt ttctgctacg gttgtaattg caggagcggt taatgcagaa
     5761 agagtaacca ccacatcatc atttacattg atcaccaaac catcgcaacg ggtatatgtt
     5821 gctcgtgctg tataagtggt gggtgatgcg gttggacaaa cgttaattga atttcctgca
     5881 ccaatctgcg ttgcaccctg ccaccatgtt acgttataaa tcggcgcacc attgggagta
     5941 aaacgccatc cttcacctgt tgcaggtgga gtccaaattg cattggtagt attccttccc
     6001 ggagcagcca taccaactgc accggtagca ttttgtatac cgatgattgc acgaccacca
     6061 ttccatgcgc aagattgttt tttgttggtg taaacttcaa ttacgttggt ggtttcatag
     6121 agaacaatct gcgaatttgt tcgtgcagat gttaaacagc tatattgaca aacatcatca
     6181 taatttacta ccagcattcg gcaaggtgca gtaccaatca ggtgaaattt tacctgacca
     6241 cactgaccgg gccattcatc cataaaaatt ccgaaaattg aattcagcgg taatgcagca
     6301 ttcggacaat caactgaaaa aaaccaggga caatatccat tggccaaacc agggttaaag
     6361 gtgagcacac catttgaacc aaccacacat tgcgtatagg tattcccata atagcaaaat
     6421 ggaaacggaa gattgattac accgctataa acgtcatcac ctccaacaac aattgctgtt
     6481 ccgcctgcct gattataggg gataaccggc gttgcacttg ttgctccaac cgtataggtt
     6541 gttgttgctc caacgtgaaa gggtgtagca gttagcgtgg tacaggcttg cgtacagggc
     6601 agcacaatat ctgcaggtgc aacaatattc ggacaacccg gagcctgggc gaataaattc
     6661 ccggcaagcg taattacacc aagggaaaag agcaataatc tcttcatagc aagtagtaga
     6721 aatggttaag aaaaggtaca atttccttac ttaacaaaga cgctatttcc cacagaggtt
     6781 gcctgatttt acaccaaaac cgaatttttg cttaccaaaa atggggatca ggatcaattg
     6841 tttcttttta aacaatttgt taaatattaa ggtaagtgaa tgatggatct ggggcaacta
     6901 aggcaaggtt tttgcgt
//
LOCUS       DAOMYG010000126         6897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3714_length_6897_cov_9.322713, whole genome shotgun sequence.
ACCESSION   DAOMYG010000126 DAOMYG010000000
VERSION     DAOMYG010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6897
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3714_length_6897_cov_9.322713"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="PK610_05465"
     CDS             complement(<1..1092)
                     /locus_tag="PK610_05465"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HRJ35413.1"
                     /translation="MAFRTIIEPFRIKMVESIKMSTTEERASYLKNAHYNPFLLNSDQ
                     VLIDFLTDSGTSAMSAEQWAGMMRGDESYAGASSWRRMYKVISELTGMPFVLPTHQGR
                     AAERILYGHLGGPGKTFISNTHFDTTRANIEFSGAEAIDIVIAEGKQPSLEHPFKGNM
                     DVNALERLIREKGPENIGGVILTVTNNSGGGQPVSLQNAKEVSAICKKYGVRLFLDCC
                     RIAENAYFIKHREEACAHMSYKEIARTLFALTDGAIMSAKKDALVNMGGFLSLKDEEL
                     ADACKNLLIITEGFTTYGGLSGRDMDAMAIGLEEVFDPDYLHYRIRSTAYLGEHLDKM
                     GIPVMKPIGGHAVYVDAKAFYPHIPAHEYP"
     gene            complement(1299..1643)
                     /gene="rplT"
                     /locus_tag="PK610_05470"
     CDS             complement(1299..1643)
                     /gene="rplT"
                     /locus_tag="PK610_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRJ35414.1"
                     /translation="MPRSKNKVASKQRRKKVLDSAKGYFGRAKNVYTVAKNVVEKAGV
                     HAYSGRKQKKRLYRSLWIQRINAGVRPHGMSYSEFIGKATAKGITLNRKALADLAMNN
                     PEAFKAIVDQVK"
     gene            complement(1754..1948)
                     /gene="rpmI"
                     /locus_tag="PK610_05475"
     CDS             complement(1754..1948)
                     /gene="rpmI"
                     /locus_tag="PK610_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRJ35415.1"
                     /translation="MPKMKTNSSAKKRFKVTATGKVKRKHAFKRHILTKKSTKAKRAL
                     THSGLVHDADMNNVKAMLVM"
     gene            complement(2111..2614)
                     /gene="infC"
                     /locus_tag="PK610_05480"
     CDS             complement(2111..2614)
                     /gene="infC"
                     /locus_tag="PK610_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018943135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRJ35416.1"
                     /translation="MHKINNRITAREVRVVGEGIEPQVYNLATALRLAEERELDLVEI
                     SPTAVPPVCRIVDYKKFLYELKKKQKEQKAKQIQVTVKEIRFGPNTDEHDFNFKLKHA
                     QEFLTEGNKVRAYVFFKGRSIVFKERGEILLLKFAQALEEIGVVEKMPSLEGKKMFIL
                     INPKKKK"
     gene            complement(2692..4638)
                     /gene="thrS"
                     /locus_tag="PK610_05485"
     CDS             complement(2692..4638)
                     /gene="thrS"
                     /locus_tag="PK610_05485"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRJ35417.1"
                     /translation="MIKIKLPDGSIREVAAGTSALAIAQGISEGLARNVLAAEVNGQV
                     VDATRSINEDATLKLLTWDDQNGKSTMWHSSAHLMAEALESFYPGVKFGIGPPIENGF
                     YYDVDFAGQAFSDSDLKKIEEKMLELARQKNPYVRKEVSKKEAVEYFLQKGDEYKLDL
                     LEGLEDGTITFYAQGAFTDLCRGPHIPNTGFIKAVKLLSIAGAYWKGDEKNKQLTRIY
                     GITFTKAKDLEEYLLMLEEAKKRDHRKLGKELELFTFSEKVGMGLPLWLPKGTALRER
                     LEQFMRSAQKKAGYQQVVTPHIANKNLYVTSGHYEKYGKDSFQPIQTPAEGEEFFLKP
                     MNCPHHCEIYKAVPRSYKDLPLRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCR
                     PDQVEEEFKKVIDLVLLVFKALDFNDYVAQISLRDPENPGKYIGSTENWEKSEAAIIK
                     AANEKGLKTVVELGEAAFYGPKLDFMVRDAIGRKWQLGTIQVDYNLPERFELEYTGSD
                     NAKHRPVMIHRAPFGSLERFVAVLIEHCAGNFPLWLTPDQAIIMPISEKYNDYAHEVL
                     QLLNNYDIRSLIDERNEKIGKKIRDAEVKKVPYMLIVGEKEQADGMVSVRRHGEGDKG
                     SFTPEQFAKMVNDDIASRLENLTK"
     gene            complement(4766..5329)
                     /locus_tag="PK610_05490"
     CDS             complement(4766..5329)
                     /locus_tag="PK610_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35418.1"
                     /translation="MMKRLVSFVFTASLVAIVGYSAAQVLNKKADEKPDPRVKAQENA
                     TKVTAYKQKSRDNLKPYRYDGTNVTHFSYSGFTQKREIEVLLFNGNEYRLVFNAEGAT
                     KPLDIEIYDKPSDNQGRVKLHEVKSVTGQENISIQTDVLNAKYKELKNSKDVVLKRIY
                     VDYIISANDTREPASGFMVLTYGYKNV"
     gene            complement(5341..6198)
                     /locus_tag="PK610_05495"
     CDS             complement(5341..6198)
                     /locus_tag="PK610_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35419.1"
                     /translation="MKFRIFSHFVPVAAASCLLLSSCGGGEEQTTVPVADTLEETIEV
                     DTMSSYSLLPSPLQVAMIFDKSGMGFEGSFTNKLDAVSKYSTNWEKSINFGVYSADLA
                     YAVLNERSKEAGEIIKVVRDLSSEIGLSTVFDSEDVLTRFEKNLGNKDSVLDILFVIE
                     EKTDDYIDENGEHELGNIMFAGAWIEGMYIGAQSSLKEKKKDVGAKLSEQMIICTNLI
                     EALSMDAPNADKDALIADLKDLDATYKGLGSVKGLEGDEAYNVNLTEAEIKTLAEKLI
                     ALRNKIVKA"
     gene            complement(6258..>6897)
                     /locus_tag="PK610_05500"
     CDS             complement(6258..>6897)
                     /locus_tag="PK610_05500"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRJ35420.1"
                     /translation="RSSGPELTRQFFALMAINKFLPLTNSISNASGNALSGGKSTVSE
                     MISSQMSNWLSQISDEFDIGLNYRPGDEISSEEIAVAFSTQLLNDRLTVSTNVGVAKG
                     NSANRNPNQLIGDFNVEYKINKDGTFRVRGYNESNEFDITRTAQAPFTQGVGVYYTEE
                     FDKLSDVKLLRKIRSWFRKKEDYKHHDDNSESRVPDQKGEPGLAEEPRLQKK"
BASE COUNT         1731 a         1608 c         1462 g         2096 t
ORIGIN      
        1 gggatattcg tgtgcgggaa tgtgcggata aaacgccttt gcatccacat acacggcatg
       61 tccacctatg ggtttcatta cgggtattcc cattttatcg aggtgttctc caaggtaagc
      121 ggtgcttctg atgcggtagt gcaaataatc tggatcgaat acttcttcca atcctatcgc
      181 catggcatcc atatctcttc ccgataatcc tccataagtt gtaaatccct ctgtaatgat
      241 gagcaaattc ttacaggcat cggccagttc ttcatctttc agtgagagga atcctcccat
      301 gttgaccaat gcatcttttt tggcactcat aatcgcacca tcggtcagcg caaacaaagt
      361 ccgcgcaatt tccttgtagc tcatgtgagc gcaggcttct tcgcggtgtt tgatgaaata
      421 tgcattttct gctatacgac aacaatcgag gaataagcgt acaccgtatt ttttgcagat
      481 agcactaacc tcttttgcat tttgcaaact caccggctga ccaccaccgc tgttgttcgt
      541 gacagttaga ataacacctc caatattttc aggacctttt tcccggatca atcgctccag
      601 cgcattcaca tccatatttc ccttaaaggg atgttcgagt gaaggttgtt ttccctccgc
      661 aataacaata tcaatcgcct ctgccccgct gaattcgata ttggcacgtg tggtatcgaa
      721 atgggtattg ctgatgaacg tttttcccgg tccgccaagg tgtccgtata agatccgctc
      781 agctgctctt ccctggtgcg ttgggagcac aaaaggcatt cctgttaatt ccgatatcac
      841 cttgtacatc ctcctccagc ttgatgcacc ggcataggat tcgtcaccgc gcatcattcc
      901 tgcccactgt tctgcactca ttgcagatgt tccgctatcg gtaagaaaat cgatcaatac
      961 ctgatcagaa ttcagcagaa aaggattgta atgcgcattt ttcaaatagg aagcacgttc
     1021 ttcagtagtg ctcattttaa tactctccac cattttgatg cggaaaggct caatgattgt
     1081 ccggaaagcc ataactcaga atttgagttt gaaatacgga ttgtgcagcg aaacggataa
     1141 tgacgggaat caggattttt tcaaatggag aacagtaaac ggtgaacagt gaatagtaaa
     1201 cggtgaacga ggtaccattt gtcacctttc acttgtcacc aataaaccga aaagcccttt
     1261 tcaattgaaa agggcttttc ggtttattga atgtatggtt actttacctg atcaacgatc
     1321 gctttgaatg cttcaggatt gttcatggca agatcggcaa gagctttacg gttaagtgta
     1381 attccctttg ctgttgcttt accaatgaat tcgctgtacg acattccatg cggacgaaca
     1441 ccggcgttga tacgctgaat ccacaatgaa cggtaaagac gcttcttttg tttgcgtccg
     1501 gaataggcgt gtacgcctgc tttttctaca acgtttttgg caacggtgta gacattctta
     1561 gcacggccaa aatatccttt ggcactgtcg agcacctttt tgcggcgctg cttggatgct
     1621 actttgtttt tactacgtgg cataatttgt tacgtttttg gaagagagtg gatcctttta
     1681 ttcttgaacg gcggaccgct tgtttactgt tcttccgggt taaactttga accgtttttc
     1741 gattggtgat cgattacatt acgagcatag ctttcacatt gttcatgtcc gcatcgtgta
     1801 ccagtccgct gtgcgtaagt gcacgtttag ccttggtaga ctttttggtg agaatgtgac
     1861 gcttgaacgc atgctttctt ttcacctttc cagtagccgt caccttgaag cgcttcttgg
     1921 cactggagtt agttttcatt tttggcattg ttcttattat ttagttaacg tttactttgt
     1981 tcggtggact gaagcccact gttacagggg attcagattc cgggtgatgt ctgcttccgg
     2041 ggactgaagt cttccggtta caaggggtgc gactgcgtcg cgggtataag ggaggtaaag
     2101 actgaagcct ttacttcttt ttcttcggat tgatgaggat gaacatcttt tttccttcaa
     2161 gagatggcat cttttcaaca accccaattt cttccagcgc ctgtgcgaac ttcaggagaa
     2221 gaatctcccc acgttcttta aagacaatgg atcgtccttt gaagaacaca tacgctctaa
     2281 ccttgttgcc ttccgtcaga aactcctgtg catgtttcaa tttgaaattg aaatcatgct
     2341 catcagtatt cggaccgaat cggatctcct tcacggtcac ctgaatctgc ttggcttttt
     2401 gttccttttg ctttttcttc agctcgtaaa gaaacttttt atagtcaacg attctgcaaa
     2461 ccggtggaac ggctgttgga gaaatctcta ccagatcgag ctcacgctct tctgcgagtc
     2521 ttagggcagt ggccagatta taaacctgtg gctctatgcc ttctcctact acacgtacct
     2581 cgcgggcggt gattcggttg ttgatcttat gcaactcctc tttttgagga cgtccgcggt
     2641 tatattcgcg gggtctggtc tgtgctataa ttgttctttt ttagtgacta cttattttgt
     2701 caggttttca agtctggaag cgatatcatc atttaccatt ttcgcgaatt gctccggagt
     2761 aaatgaaccc ttatcccctt ctccatgcct gcgcacggat accattccat ctgcctgttc
     2821 cttctcaccg acgattagca tatacggcac ttttttcacc tctgcatccc tgatcttttt
     2881 gccgatcttt tcgttgcgct cgtcaatgag gctgcgaata tcgtaattat ttaacaattg
     2941 taaaacctca tgggcataat cattgtactt ctccgagatg ggcatgatga tggcctggtc
     3001 gggtgttaac cacaatggga agttacccgc acaatgctcg atcagaacag caacaaagcg
     3061 ctcgagcgat ccaaagggtg ccctgtgaat cataacgggt ctgtgcttgg cattatccga
     3121 accggtgtac tccagctcaa aacgctccgg taagttgtaa tctacctgaa tagtacctaa
     3181 ttgccacttt cttccaatgg catctcgtac catgaaatcg agttttggac cataaaacgc
     3241 tgcttctcca agctcaacaa cggtcttcaa tcccttttca ttagcagctt tgatgatggc
     3301 ggcctccgat ttttcccaat tttcagttga gccaatgtat ttacccggat tctccggatc
     3361 gcgtaatgaa atctgtgcaa cgtagtcatt gaagtccaat gccttaaaca ccaataacac
     3421 caaatcgatc acctttttga attcctcctc aacctggtcg gggcggcaaa aaatatgggc
     3481 atcgtcctga gtaaaaccac gaacgcgggt cagtccgtgt aactcaccac tttgttcata
     3541 gcggtacaca gtaccaaact cagccaaacg caatggcaaa tccttatacg aacgtggtac
     3601 cgctttatat atttcacaat ggtgaggaca gttcattggc ttcaggaaaa actcttctcc
     3661 ttcagcaggt gtttgaatcg gctgaaatga atcctttccg tacttctcgt agtgaccgga
     3721 agtgacgtat aaatttttgt tggcgatatg aggagtaaca acctgctggt atcctgcctt
     3781 cttttgtgca gatcgcatga attgttccaa gcgttcgcga agagcagttc ccttgggtaa
     3841 ccaaagcggt aatcccattc caactttttc ggaaaaagtg aacagctcaa gttctttacc
     3901 cagtttgcgg tgatcccgtt tttttgcttc ttcgagcatc agcaaatact cctcgaggtc
     3961 ttttgccttt gtaaaagtga taccgtaaat gcgggtgagc tgcttattct tctcatcacc
     4021 tttccaataa gctccggcaa tactcaacaa ttttacagcc ttgatgaatc cggtattggg
     4081 aatatgcgga cctctgcaga gatcagtaaa tgcaccctgc gcgtaaaaag taatggttcc
     4141 gtcctcgagt ccttcaagaa gatcgagttt atactcatca cccttttgga ggaaatattc
     4201 gacagcctct tttttgctga cttcttttcg cacataagga tttttctggc gtgcaagttc
     4261 cagcattttc tcttcgatct ttttcagatc actatcggaa aaagcttgcc cggcaaaatc
     4321 aacatcgtag tagaatccat tttcaattgg aggaccgatt ccgaatttca ctccgggata
     4381 aaatgactca agagcctctg ccatcaggtg ggcagatgag tgccacatgg tagattttcc
     4441 gttttgatca tcccaggtaa gtaatttcag tgttgcatct tcattgatgc tccgcgttgc
     4501 atccacaact tgtccgttaa cttcggcagc aagcacatta cgtgcaagtc cttcgcttat
     4561 tccctgtgca atggccagag cagaagttcc cgcagccacc tcacgaatgg agccatcggg
     4621 taatttaatt ttgatcatgt agatagaatt ggagtgcgaa aataatgtat ttggtgaaag
     4681 gataagtgtg cattgggatt ggttagaaat gtaagcttaa aaaaaaaggc cggacaagcc
     4741 ggccttttag tactagtgac ttgtgttata cattcttgta tccatacgtg agaaccatga
     4801 atcctgaggc aggctcccgg gtatcattcg cagagataat gtaatcaaca taaatacgct
     4861 tcaaaacaac atctttactg tttttaagct ccttgtactt cgcgttaagc acatctgttt
     4921 gaatagagat attttcctga ccggtaacgc tttttacttc gtgtaattta acgcgaccct
     4981 gattgtcaga aggcttatcg taaatttcaa tatccaaagg ctttgtagct ccctcagcat
     5041 tgaataccag acggtactcg tttccgttga acaacagcac ttcaatctca cgcttctggg
     5101 taaaaccgga ataactgaag tgagttacat tggttccatc atagcggtaa ggcttgaggt
     5161 tatcacggct tttttgcttg taagcagtaa ctttggttgc gttttcctgt gccttaacac
     5221 gcggatctgg cttctcatct gccttcttgt tcagaacctg agcagcactg taaccaacga
     5281 tggctacaag tgaggcagta aatacgaatg atactaatct tttcatcata acaccgggaa
     5341 ttaggcttta acgatcttgt tacggagtgc aatcaatttc tctgccaaag tcttgatctc
     5401 ggcttcagtc agattaacat tgtaggcttc atcaccttca aggcctttta cagagcctaa
     5461 tcctttgtag gttgcatcca ggtctttaag gtcagcgata agcgcgtctt tatcagcatt
     5521 gggggcatcc atagaaagtg cctcaataag gtttgtgcag ataatcattt gctcggagag
     5581 cttagctccg acatcttttt tcttttcttt taaagatgac tgagcaccaa tgtacattcc
     5641 ttcaatccac gcaccggcaa acataatgtt acccaattcg tgttctccgt tctcatcgat
     5701 gtagtcgtcg gttttttcct cgatcacaaa aaggatgtca agaactgagt ctttgttacc
     5761 cagattcttt tcgaaacggg taagaacatc ctcggaatca aacaccgttg aaagaccgat
     5821 ctctgagctc agatcacgaa ccactttaat gatttcacct gcttctttag aacgttcgtt
     5881 aagaacagcg tatgctaaat cggctgagta aacaccaaaa ttgattgact tctcccagtt
     5941 ggtcgaatat tttgaaactg cgtcaagctt gtttgtgaaa ctaccttcga agcccattcc
     6001 ggacttatca aagatcattg ccacctgaag agggcttggc aacaggctat aggaagacat
     6061 ggtatctacc tcaattgtct cctctagcgt gtcggcaaca gggacagtag tttgctcctc
     6121 accaccaccg caggaggaaa gtaacaagca agaagctgct gctacaggta caaaatgact
     6181 gaaaattctg aacttcataa atgattgggt ttcggctagc gaatttagac aaagattcgg
     6241 actgagcgaa aaaggcatca ctttttttgc agcctgggct cttcggcaag gccgggttcc
     6301 cctttttggt caggaacacg cgactccgaa ttatcatcat ggtgcttgta atcttccttt
     6361 ttccggaacc aggaacggat tttccgcaag agttttacgt ccgaaagttt atcaaactct
     6421 tctgtataat aaaccccaac accctgagta aaaggagctt gagctgttct ggtgatatcg
     6481 aactcgttcg actcattgta tcctctaacc ctaaatgttc cgtccttgtt gattttatac
     6541 tccacattga aatcgccgat aagctggttg gggttgcggt ttgcgctgtt tccttttgcc
     6601 acacccacgt tggtacttac agttaagcga tcgttgagca actgggttga aaatgcaaca
     6661 gcaatttcct cagacgagat ttcatcgccg ggtcggtaat tcaatccgat atcgaattca
     6721 tcactgatct gtgaaagcca attactcatt tgactggaga tcatttccga aacggtagac
     6781 tttccacctg aaagtgcatt accggaagcg ttgctgatcg agttggttaa tggcaaaaat
     6841 ttattgatcg ccattaatgc aaaaaactgg cgtgtgagct ccggtcctga agagcga
//
LOCUS       DAOMYG010000127         6896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3717_length_6896_cov_10.024558, whole genome shotgun sequence.
ACCESSION   DAOMYG010000127 DAOMYG010000000
VERSION     DAOMYG010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3717_length_6896_cov_10.024558"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PK610_05505"
     CDS             <1..851
                     /locus_tag="PK610_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35421.1"
                     /translation="CALMKKVSLHQTMKHLLLPILFFLSATNSFSANLVLEGNYQGKN
                     LYVQNPFAAGNIGFCTIRVMVNDNVTTDEVNSSAFEIDLSLFKLNVGDAIVVKIEHKE
                     GCVPKVLNPEVLKPKSTFEVTDMQVSPSGKLTWKAKGENGKLPYVIEQKVWNKWIPVG
                     EVDGNGTPAEHAYEFQLTPHSGENTVRVKQVDYTNRPRYSQPKTFSDPSVKEVDFVPK
                     KVKNSPIKFVEAGTEKGVKTRYEIYDSYGNIVKKGYGDEVDVSGLKSGAYYINYDNKD
                     AQFIKN"
     gene            925..998
                     /locus_tag="PK610_05510"
     tRNA            925..998
                     /locus_tag="PK610_05510"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:959..961,aa:Ile,seq:cat)
     gene            1075..1683
                     /locus_tag="PK610_05515"
     CDS             1075..1683
                     /locus_tag="PK610_05515"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRJ35422.1"
                     /translation="MIDAIILDLGGVLLNIDYQKPIDAFKALGVEVLYSQKDQGKLFD
                     DLETGKISSADFRNEVRKLSSDALSDQQIDKAWNSILLDLPENRVELLYKLKQKFPLY
                     LLSNTNEIHAQSFEEEIIREYGHNVFEEVFSGIYYSHRIQKRKPNAEAFLHVLKENGL
                     HEATTLFIDDSPQHIVGAKNLGIQAYQLNGELEFLLKEIGVI"
     gene            complement(1684..2085)
                     /gene="mce"
                     /locus_tag="PK610_05520"
     CDS             complement(1684..2085)
                     /gene="mce"
                     /locus_tag="PK610_05520"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRJ35423.1"
                     /translation="MNKVEHIGIAVKSIQESARVFELLLGAPAYKEEQVESEGVKTAF
                     YQVGETKIELLEATKPDSAIAKFIEKKGEGVHHIAFDVTDIEKEISRLSAEGFELIHK
                     TGKDGADNKIIAFLHPKSTNGVLVELCQEKL"
     gene            2214..2525
                     /locus_tag="PK610_05525"
     CDS             2214..2525
                     /locus_tag="PK610_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRJ35424.1"
                     /translation="MHTGLRIKAARIASGLRQKDLALRVDRSVHLIAYIERTGKISAT
                     MKKRIYLSLNMNEEEYSESVLPLKNKVLVLEENLLLAKQSLLLMSLQMKALEERIKDH
                     H"
     gene            2564..3451
                     /locus_tag="PK610_05530"
     CDS             2564..3451
                     /locus_tag="PK610_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35425.1"
                     /translation="MKSIILYLLFSIQPDTSAAVRSVVSVPEILLHRAEALWVEDRSS
                     IKKALASKITSHVPLIVHVFVPLCDNLNQGIVPVNSKLGDGMNLNTNLYWGAGYGVRS
                     YFKLKTDWKLSLQQANPDSNILERVVFRKTMSNGTAIIMIADAYRGDRMKQCLRSYMH
                     SLAGILNDSISLSDKQYPFHSGADLLIFNGHNGLMDDTLAPVLNADKRPKDAMIIACY
                     STSYFRPYLQRSGGYPLVTTTGLLAPEAYVIHAAIENWALQKSEKEIRIAAGDAYYAK
                     HPSSTQGGCRGLFRTGWAD"
     gene            3495..4520
                     /gene="galE"
                     /locus_tag="PK610_05535"
     CDS             3495..4520
                     /gene="galE"
                     /locus_tag="PK610_05535"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016419658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRJ35426.1"
                     /translation="MSTGKKIIVTGGAGFIGSHTVVELCKAGFHPVIVDDFSNSEPFI
                     VERLNELCGTTLPFYECDCTDKNAFRKIFEKERPSGIIHFAACKAVGESVAQPLKYYH
                     NNIGSLVVVLELMKDFGVNDLVFSSSCTVYGQPEQVPVSESSPSKQAMSPYGYTKVVC
                     EQIIEDVIASGQALRCVLLRYFNPIGAHPSGRIGELPRGVPNNLIPYITQTAAGQRSS
                     LTIHGNDYSTVDGTCIRDYIHVVDLADAHVKALEWIHSETDEPLREVFNVGTGKGETV
                     LEVVNTFKKISGIDVPYTVGPRRSGDVEQIWASTEKVEQLLGWKARLSLADALRDAWN
                     WQKQLKA"
     gene            4517..5209
                     /locus_tag="PK610_05540"
     CDS             4517..5209
                     /locus_tag="PK610_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRJ35427.1"
                     /translation="MRGILVFVFLLNTPIAFCQISAEEAYKGIIPGEYNSPERMHRWM
                     MDVYQDRWEPLPNGIQSKWYSFGIAASRMIDIPLNKRSTIAFAFGPGIGSHNVHHNGA
                     FTNTIDTSGIRRYRIEPLPTGYSYKRNKIRLTLIELPFQLRIRSKRDGFFFYPGFKAG
                     WLVGNHSLIVDDQGKYKRYNYKGFEKLQYGPTLHMGYNRLALYAYYSLTPVMKEGEDF
                     RTFSIGLSLNFF"
     gene            complement(5206..5931)
                     /gene="rnc"
                     /locus_tag="PK610_05545"
     CDS             complement(5206..5931)
                     /gene="rnc"
                     /locus_tag="PK610_05545"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRJ35428.1"
                     /translation="MFSFFRYKDPDEKAISAFVRNKFGIRIHKADLYLQAFLHKSHLR
                     DEPKAHERSNERLEFLGDAVLDAIVAEYLYRHFPSETEGFLTKIKSRIVKRETLNMLG
                     EKLNLDSHIRHTRFGINNPKSLLGNAFEALIGAIYLDQGFEVTRKVVLHQILEKYIDL
                     NAIVQVETDYKSRIIIWSQRERRRIDFVLLLEENLGAEMRYEIELRIDQEPFSKASAR
                     TKKEAEQTASRDAWVTLFPGETD"
     gene            complement(5937..>6896)
                     /locus_tag="PK610_05550"
     CDS             complement(5937..>6896)
                     /locus_tag="PK610_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HRJ35429.1"
                     /translation="DRAGVIWGSGIGGLKTFQDECQGFFAGDGTPRFNPFFIPKMIAD
                     IASGHISITYGFRGPNFTTVSACASSTNAINDAFTYIRMGKADIIITGGSEATVHETG
                     VGGFNALHALSTRNDSPETASRPFDKDRDGFVLGEGAGCLILEEYEHAKKRGAKIYAE
                     VVGGGMSADAHHLTAPHPEGLGARNVMKYALSDAGIRVEDIDYINVHGTSTPLGDVAE
                     TKAILGVFGDHSYKLNISSTKSMTGHLLGAAGAIEALACVLAVSKNIIPPTINHFTDD
                     PDLDPRLNLTFHKPQERVVNYALSNTFGFGGHNASIILKKFQG"
BASE COUNT         1979 a         1544 c         1455 g         1918 t
ORIGIN      
        1 attgcgcctt aatgaaaaaa gtgtcattac atcaaacgat gaaacacctg cttcttccaa
       61 ttctcttttt cctgtctgcg acgaacagct tttctgctaa tctcgtactg gaagggaact
      121 accagggtaa gaacttatac gttcaaaacc ccttcgctgc cgggaacatt ggtttctgca
      181 ccattcgtgt tatggttaac gataatgtaa ccaccgatga agttaactct tctgctttcg
      241 agatcgactt gtcgctcttt aagctaaatg ttggtgatgc cattgttgtg aaaatcgaac
      301 acaaagaagg ttgcgtgcca aaagtcctta acccggaagt attaaaacct aaatctacat
      361 tcgaagttac cgacatgcag gtgagtcctt ccggtaagct tacctggaaa gcaaaaggag
      421 aaaacggaaa attaccttac gttatcgaac agaaagtctg gaacaaatgg attcctgttg
      481 gagaagttga cgggaatggt acaccggcag aacacgctta cgaatttcaa ttgacaccac
      541 acagcggcga aaacaccgtg cgcgttaaac aggtcgacta caccaaccgt cctcgttatt
      601 ctcaacccaa aacgttctct gatccttccg ttaaggaagt tgatttcgtt cctaaaaaag
      661 taaagaattc ccccattaaa tttgttgaag ccggaactga aaaaggagtt aaaacacgct
      721 acgagattta cgactcttac ggtaacattg taaagaaagg ttacggagat gaagtagatg
      781 tttccggtct taaatccgga gcttactata tcaactacga taataaagac gctcagttca
      841 tcaagaatta atcgtccact taaagatacc agaaccccgg accacaaatc cggggttttt
      901 ttatatttgc actcctttaa taccgggcct atagctcagc cggttagagc atctgactca
      961 taatcagagg gtccctggtt cgagcccagg tgggcccacc cgtgtaagaa agaaccctgt
     1021 tcgtattgcg aatagggttt tttgcttttg attccgttct gtttctttgc gcttatgatt
     1081 gacgcaataa ttttagatct gggtggtgtt ctgctcaaca tcgattacca aaaaccaata
     1141 gatgcattca aggcattggg agttgaagtg ctgtattcac aaaaagatca aggtaaatta
     1201 ttcgacgatc tggaaaccgg taaaatctct tctgcagact ttcgcaatga agtaagaaaa
     1261 ttaagcagcg atgcactcag tgatcagcaa atcgacaaag catggaatag tattctgctc
     1321 gacctgcccg aaaacagggt tgaacttttg tataagctaa aacaaaaatt tcccctgtac
     1381 ttgcttagca ataccaacga aattcatgct caatcgttcg aagaggaaat aatccgtgaa
     1441 tacggacaca atgtttttga ggaagtattc tccggtatat actattccca ccgaattcaa
     1501 aaaagaaaac cgaatgcaga agcctttctc catgtgctta aagaaaacgg tctccatgaa
     1561 gcaacaacat tgttcatcga cgactcaccg caacacattg tcggtgcaaa aaacctgggt
     1621 atacaggctt atcaattgaa tggcgaattg gagttcttgc taaaagaaat cggcgtaata
     1681 taatcaaagc ttttcctgac aaagctcaac caaaacacca ttggtggact ttggatgcaa
     1741 aaacgctatg atcttattat ctgcgccgtc ttttccggtt ttgtgtatca attcaaaccc
     1801 ctcagcgcta agccttgaaa tttccttttc aatgtcagtc acatcaaaag caatatgatg
     1861 tactccttct cccttctttt caataaattt agcaatagcg ctgtcgggtt ttgtcgcctc
     1921 aaggagttca atctttgttt cgcccacctg gtaaaaagct gtttttaccc cttcgctttc
     1981 aacctgttct tccttgtaag caggcgctcc cagcaaaagc tcgaaaaccc gtgctgactc
     2041 ctgaatgctt tttacagcta ttccaatatg ctcaactttg ttcatcttcc aaaaatacag
     2101 ctcttttaat tgaacggtat tgcttaaccg gacaaaatct gtctgaatta gacaaaatct
     2161 gtcagtagtt attcggttta ttctgcgtga tttgtactac taaaaaccaa accatgcata
     2221 caggccttcg gatcaaagca gccaggattg catcaggact ccggcaaaaa gatttggccc
     2281 tcagggttga ccgttcagtg catctcattg cctatatcga acgaaccggg aaaataagtg
     2341 ccacaatgaa aaagagaata tacctctctt taaacatgaa cgaggaagaa tattctgaaa
     2401 gtgtactccc gctaaagaac aaagtattgg ttctcgaaga aaacctcctt ctcgccaagc
     2461 aatcgcttct attgatgagt ttgcaaatga aagcactcga agaaagaata aaagaccatc
     2521 actaagacag atacgaaaaa ccggtttcat ccgttttaca gctgtgaaat caattattct
     2581 gtatctgctg ttcagtattc aacccgacac ctcggcagcc gttcgatcag tggtaagtgt
     2641 tcccgaaatt ctcttgcatc gggcagaagc attgtgggtg gaagaccgct cctcaattaa
     2701 aaaagcactg gcaagcaaaa taacttcaca cgttcctctg attgtacacg tatttgtgcc
     2761 cctgtgcgac aacctcaatc agggcatcgt tcctgtgaat tccaagcttg gtgacggaat
     2821 gaatcttaac accaatctct attggggagc ggggtacggt gttcgttctt acttcaagtt
     2881 aaaaacagac tggaaactct ccctccagca ggcgaatccg gacagcaata ttctggaaag
     2941 agttgttttc cgaaaaacca tgagcaatgg aacagcaatt attatgattg cagacgccta
     3001 tcggggagat cgcatgaaac aatgcttaag aagctatatg catagtttgg caggcatact
     3061 taatgacagc attagccttt cagataaaca atatcccttt cacagtggcg ctgatttgtt
     3121 aatctttaac gggcataacg gactaatgga tgatacactt gcgccggttc tcaacgcaga
     3181 taaacgacca aaagacgcca tgattatcgc ctgctattcc acctcctatt tcaggcccta
     3241 tctgcagcgt tcaggagggt atccgcttgt aaccactacc ggtcttttgg ctcccgaagc
     3301 ttatgttatt catgctgcaa ttgaaaattg ggcacttcag aaatcagaga aagagattcg
     3361 cattgctgca ggagatgctt attacgccaa acatccgtcc tcaacacaag gaggctgcag
     3421 aggattgttc cgtacaggtt gggcggatta attcatacat ctccttttgt attatctttg
     3481 gtcggaaacg caagatgtcg acaggaaaaa aaataattgt aacaggcggt gccggtttta
     3541 ttggttcgca tacagtagtt gaattatgca aagccggttt tcatcctgtt atcgtagatg
     3601 atttttccaa ttcggaaccc ttcatcgttg agcggttaaa tgaattatgc ggcaccacat
     3661 taccatttta tgagtgtgat tgcacggata aaaatgcatt tcgaaaaatt ttcgaaaagg
     3721 aacgaccttc ggggatcatc cattttgctg cctgcaaagc agttggagaa tctgtagcac
     3781 aacccttaaa atactaccac aacaatattg gctcacttgt ggtagtgctc gaattaatga
     3841 aagattttgg ggtgaacgat cttgtgtttt cttcctcctg cacggtttat ggccagcctg
     3901 aacaggtgcc cgtttcagaa tcctccccct caaaacaggc aatgtcgccc tacggataca
     3961 ccaaagttgt ttgtgagcag atcatcgaag acgtgattgc ttcaggacag gctttgcgtt
     4021 gcgttttatt gcgctatttc aatccgatcg gcgctcaccc ctcaggaagg attggagagc
     4081 ttcccagagg tgttccaaac aatctgattc cctacattac ccaaacagcg gcaggacaac
     4141 gatcttctct tactattcac ggaaatgatt attccaccgt tgatggcacc tgcattcgcg
     4201 attacatcca tgttgttgac cttgcagatg cccacgtaaa ggcactggaa tggatccatt
     4261 ctgaaacaga tgaaccctta cgtgaggtct tcaatgttgg aaccggcaag ggagaaactg
     4321 tgcttgaagt cgtaaacact tttaagaaga ttagtggaat tgatgttccc tatacggttg
     4381 gtccacgaag atccggagat gttgagcaga tctgggcctc aacagagaaa gttgaacaac
     4441 tactgggctg gaaagccaga ctcagtttag cagatgcttt gcgcgatgcg tggaattggc
     4501 aaaaacaact caaagcatga ggggaatatt agtttttgtt tttcttttga atacgcccat
     4561 tgcgttttgt cagatcagtg cagaagaagc ctataaagga atcattcccg gagagtacaa
     4621 ttcaccggaa cgcatgcatc gctggatgat ggatgtttat caggatcgtt gggagccact
     4681 cccaaatgga attcagtcga aatggtattc attcgggata gccgcatcac gcatgattga
     4741 tattccacta aataaaagaa gcaccattgc ctttgccttt ggtccgggaa tcggttcgca
     4801 taatgttcat cataatggtg ctttcaccaa cacgattgat acttccggaa ttcgtcgcta
     4861 taggattgag ccacttccta cagggtattc ctacaagcga aacaaaatcc gattaaccct
     4921 gattgaactt ccctttcagt tgcgcatcag aagcaagcgt gacggattct ttttttatcc
     4981 tggatttaaa gcgggatggc tggttggtaa tcattcctta attgtagatg accagggaaa
     5041 atacaaacgc tacaattata aagggttcga aaaacttcag tatggtccta cccttcatat
     5101 gggatacaac cgactggctt tatatgcata ctattcactt acaccggtga tgaaagaagg
     5161 agaagatttc agaacctttt ccattggttt atcgcttaac tttttttagt cggtctctcc
     5221 cggaaacaac gtaacccatg catctcttga tgctgtttgt tctgcttctt tttttgtgcg
     5281 tgcgcttgct tttgaaaacg gctcctgatc aatccgcagt tcgatttcat aacgcatttc
     5341 cgcaccgaga ttttcttcca acaacaaaac aaaatctatt cttcgccgct cgcgttggga
     5401 ccaaattatg atgcggctct tataatcggt ttcaacctga acgatagcat tcagatcgat
     5461 gtatttctca agaatctgat gcagcactac ttttcgtgtg acctcaaaac cctggtcgag
     5521 ataaatggca ccaatcaagg cttcaaatgc atttcccaat agcgattttg ggttgttgat
     5581 tccaaaccgt gtatgacgga tatgactgtc taaattcagt ttttcgccaa gcatgtttag
     5641 tgtttcccgt ttaacaattc gcgatttaat ttttgtcagg aaaccttctg tttcggaagg
     5701 gaaatggcgg tacaaatatt cagctacaat ggcatcgaga acagcatcac caagaaactc
     5761 caatcgttca ttgcttcgct cgtgtgcttt gggttcatcc cgcaaatgtg atttatgcag
     5821 aaatgcctgc agatagagat ctgccttgtg tatacgaatg ccaaatttat tcctgacgaa
     5881 agcgcttatt gctttttcat ccggatcttt atagcgaaag aaggaaaaca atggatttac
     5941 ccttggaact tcttaaggat gatggaggca ttgtgtccgc caaatccgaa ggtattgctc
     6001 aatgcgtaat ttacaacgcg ctcctgaggc ttatggaagg tgagattcag acgtggatcc
     6061 aagtccgggt cgtctgtaaa atggttgatg gttggtggaa tgatattctt gcttaccgcc
     6121 agcacacagg ccaaagcttc aatggctccg gcagcaccaa gtaagtgtcc ggtcattgat
     6181 tttgttgagc tgatgttgag cttgtacgaa tgatctccga aaacaccaag aattgctttt
     6241 gtttcggcaa catctcccaa aggagttgag gttccgtgaa cgttgatgta atcgatatct
     6301 tctacgcgaa taccggcatc gctaagtgcg tatttcatca cattgcgcgc accgagacct
     6361 tcagggtgtg gagcggtaag gtgatgggca tctgccgaca ttcctccacc aactacttca
     6421 gcataaattt ttgcgccgcg ttttttggca tgctcatact cttccaaaat caaacatccg
     6481 gctccttctc ccaatacaaa gccatctcga tccttatcaa aaggacgtga agctgtttcg
     6541 ggtgaatcat tgcgggttga taaggcgtga agagcattga atccacctac tccggtttca
     6601 tgcacagttg cttcagagcc tccggtaata atgatatcag ccttacccat acggatatag
     6661 gtgaaggcat cattgattgc attcgtagag gaagcacagg cagaaacagt ggtgaaattt
     6721 ggtcccctga aaccataggt gattgaaata tgccctgaag caatatcggc aatcatctta
     6781 ggaatgaaaa agggattgaa acgcggagtt ccgtcaccgg caaaaaagcc ctgacattcg
     6841 tcctgaaaag ttttcaagcc accgatacca gatccccaga tcactcccgc acggtc
//
LOCUS       DAOMYG010000128         6877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3736_length_6877_cov_8.816916, whole genome shotgun sequence.
ACCESSION   DAOMYG010000128 DAOMYG010000000
VERSION     DAOMYG010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3736_length_6877_cov_8.816916"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PK610_05555"
     CDS             complement(<1..637)
                     /locus_tag="PK610_05555"
                     /inference="COORDINATES: protein motif:HMM:NF020442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HRJ35430.1"
                     /translation="MTGFISSVSEFASTISRFSDHSTLYRGVSKSEYALIPSIGRENF
                     KLLNTLPESTLQKFEKDLILLFQANSYQHLRHNNLPLLELLAFGQHHGLKTRLLDWTK
                     SALAALFFAVEKNPNDNGKVFLFKSRSHKYLNSDSVCEIKDLADINQNSMFIPLNSTP
                     RINVQQGLFMVFDKPNEPYENDKITYIEIHHKDKPKIQKEFYLLGINNYTLF"
     gene            complement(735..2021)
                     /gene="eno"
                     /locus_tag="PK610_05560"
     CDS             complement(735..2021)
                     /gene="eno"
                     /locus_tag="PK610_05560"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRJ35431.1"
                     /translation="MSTIAQIEARQILDSRGNPTIEVDVITANGILGRAAVPSGASTG
                     AHEAAELRDGDKGIYLGKGVTKAVANVNDALNKELHGVPVFNQNIIDRLMIQLDGSSN
                     KSKLGANAILGVSLACAKAAAQEAGLPLFRYIGGVNATTLPIPMMNILNGGAHADNKI
                     DIQEFMVMPVGAETFSEGLRMGTEIFHNLKAVLKKAGHNTNVGDEGGFAPNLGSNEEA
                     IEMVLRAIESSGYKVGEDVYIALDAASTEFYNAEKGLYIFESTGKQMSSDEMVAYWAD
                     WAKKYPIISIEDGMAEDDWAGWKKLTDTLGSEIQLVGDDLFVTNTERLAKGIEGGIAN
                     SILVKVNQIGTLTETIEAVDMAKSWAYTSVMSHRSGETEDSTIADLAVALNTGQIKTG
                     SASRSDRIAKYNQLLRIEEMLGPGAYYPGKDFRYVK"
     gene            complement(2092..3216)
                     /gene="carA"
                     /locus_tag="PK610_05565"
     CDS             complement(2092..3216)
                     /gene="carA"
                     /locus_tag="PK610_05565"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRJ35432.1"
                     /translation="MKYQVKPSALLVLEDGTVFHGKAAGIIGTTTGEIAFNTGMTGYQ
                     EIFTDPSYLGQIVVMASPHIGNYGVHKDEVESDSVKISGLVCKKFSVNFSRPSGESSL
                     QTYFERDNIVGICEVDTRALVRHIRNAGAMNAIISSEILDPEILKQKLKTVPSMDGLE
                     LSSRVSTKEAFTSGDAASPFKVALLDLGVKKNIVRCLVERGCYVKVFPMQASLDEMLA
                     FEPNGFMLSNGPGDPGVMSDTIEKVKAIVATGLPIFGICLGHQILALSQGLPTKKMHN
                     GHRGINHPIKNLITGKSEITSQNHGFVVDKEALSNRSDIEMTHVHLNDGTLAGIRLKG
                     KPVFSVQYHPEANPGPYDARYLFDDFVHNMKVHSKIKVNN"
     gene            3426..>6877
                     /locus_tag="PK610_05570"
     CDS             3426..>6877
                     /locus_tag="PK610_05570"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ35433.1"
                     /translation="MKTIEISIFTRMRHLFTIVLVFMLLAPSFGHEHGNDERYYRFLP
                     NQGQWEHAIRYKAKINSGALFLLEDSWVFHFQDNSVLLKAHLGEPVKPEDDRIFGQSY
                     KVKFIGAKTKGFEQKEKGEEYYNFFLGNDKDKWASKVFPYKHITYNNIYQGIDLRLYS
                     KDEQLKYDWIIAPGSDPSLIVQEYEGVEKIRITTIGDLEIKTTVGTVIEKAPKAFQII
                     RGKKVEVPCRFTLKGKQLSYEFPNGYDRSFELIIDPVLVFGSYTGSTADNFGMTATYS
                     SQGHLFTGGMVYNIGYPTTLGAYSVTSNVNNSVYGVTDIVITKFSPNGTSLVYSTYLG
                     GGTTNQGTETVHSLICNANDELFLFGVTSSTDFPTTAGAYDQTFNGGSFIGFYQNGSY
                     FYNAGTDIFVSKLSFDGANLLASTYIGGTGNDGVNYNQNFQQGVNWVSNYDSLQTNYG
                     DQFRGEIMVDDLGNCYVASMTKSSDFPIVNAYQSTSGGQQDGVVFKLDQNLSNLQFST
                     YIGGSNKDACYGIKVDAAYNVFVTGGTASANFPSTPGTLHTTYQGGKTDGYVIKLSPT
                     GNTLLASTFIGTNAYDQTFFIELDRFGSVYVLGQTSSPAGMPPVNVNYSNANSGQFIV
                     KMNNALNTLEYRTLFGNGSGNFNISPSAFLVDVCGNVYVSGWGANILQNNPLTGMPVT
                     ANAFQPGSGDGFNFYLIVFSRNIDNLLYATYFGGNQSREHVDGGTSRFDANGVVYQSV
                     CAGCGSNDDFPTTPGAWSNINLSTNCNNGVFKYDMEIEPEADFVTDKLEGCSPLTIHF
                     DNTSPLYSEFLWDFGGGDTTSTIVNPVYTFTTPGTYTVYLIVRDSICLLLDTAEKVIT
                     VYPPLQLFTSDTVICSPQTVALLANSNGTSTNFYWSSNGNFSDTLNAPITDSTLSVFV
                     SGTTNYYVNVANTWCSYTDTVTVFVPDLDISITSINGICTGDTVALTVTNNTPLFPLS
                     YNWFPDSLILSGDGSSTVLVSPDYDNWIYVTGTTAEGCTITDSTFINVDGPPTGLVSA
                     TADQYAILAGASTVLHAQPNGYSYTWTPAFTLDNPNSQHPTATPTETTIYTVTISNGG
                     CSLTDTVRVRIVEIFCGEPNIYIPNAFTPNGDGENDFLFVRGNNITKLVFRVYDRWGE
                     KVFET"
BASE COUNT         1948 a         1401 c         1417 g         2111 t
ORIGIN      
        1 ggaaaagggt atagttgtta attcctaata gatagaattc cttttgaatt tttggtttgt
       61 ctttatgatg aatttcaata taagtgattt tatcgttttc ataaggttca tttggtttgt
      121 caaataccat aaataatcct tgttgcacat taattctagg agtactgttt aagggaatga
      181 acattgaatt ttgattgata tctgctaaat ctttaatttc acaaacacta tctgaattca
      241 aatatttatg tgacctagat ttgaacaaga aaacctttcc gttatcatta ggatttttct
      301 caacagcaaa aaatagagct gcaagggcac tttttgtcca atccaataac ctagttttta
      361 aaccatgatg ttggccgaaa gcaagtaact caagtaatgg taaattattg tgccttagat
      421 gttggtatga attggcttgg aaaagtagta tgagatcttt ttcaaatttt tggagggttg
      481 attcaggtag tgtgttcaga agcttgaaat tttcccttcc aattgatgga attaaagcat
      541 attctgattt ggaaacaccc ctatatagag ttgagtgatc tgaaaatctg ctaattgttg
      601 aggcaaattc agaaacactt gaaataaatc cagtcatttt ttgtaaaaat caagtcctaa
      661 cccaagatac gagaatttgg ataaaaaaac gccccttctg tcgaagaggc gtttttgatt
      721 ttataagaat ttgattattt cacatacctg aaatccttac cgggataata agcacccgga
      781 ccaagcattt cttcgatacg caataactga ttgtatttag cgatccggtc ggagcgtgag
      841 gctgatccgg ttttaatttg cccggtgttt aatgcaacag caagatctgc aatggtactg
      901 tcttccgttt ctccggagcg gtgactcatt acagaggtgt aagcccaact ttttgccata
      961 tcaactgctt caattgtttc cgtcaacgtt ccaatctgat ttactttaac caggattgag
     1021 tttgcaattc cgccttcaat tccctttgcc agtcgctcag tattggttac aaataaatca
     1081 tcacccacaa gctgaatttc gctgccaagt gtatccgtta attttttcca acctgcccaa
     1141 tcatcttctg ccattccatc ttcaatagaa atgatgggat atttcttcgc ccaatcagcc
     1201 caataggcta ccatttcatc cgaactcatt tgctttcctg tagattcaaa aatgtacagg
     1261 cctttttctg cgttgtaaaa ctccgtgctt gctgcatcaa gggcaatgta aacgtcctcg
     1321 cccactttat agcctgaact ttcaatggca cgtaacacca tttcaattgc ttcttcattg
     1381 gaaccaaggt taggtgcaaa accgccttca tctccgacgt ttgtattgtg accagctttt
     1441 ttcaaaacag ctttgaggtt gtgaaaaatt tctgtcccca tgcgcagacc ttcagaaaaa
     1501 gtttcagcac ctacaggcat aaccatgaat tcctgaatat cgattttgtt atctgcatgc
     1561 gcacctccat tgaggatatt catcatcgga attggcaatg tagttgcatt cactccgccg
     1621 atgtagcgga acaatggtaa acctgcttct tgagctgccg ctttcgcaca tgcaagggaa
     1681 acacctaaaa ttgcatttgc acccagtttc gatttgttgc tggatccatc caactgtatc
     1741 atcaatcggt caatgatatt ctgattgaaa accggaacac cgtgtaattc tttattgagt
     1801 gcgtcattaa cattggcaac agcttttgtc acaccttttc ccaggtagat gcctttatct
     1861 ccatcgcgta attcagcagc ctcgtgtgca cctgtagaag caccggaagg aacagcagca
     1921 cggccaagaa ttccgtttgc tgtaattaca tctacttcaa ttgtaggatt tccgcgagaa
     1981 tcaagaatct gacgagcttc aatttgtgca atggttgaca tggttggttt gttgttagtt
     2041 atcggttatc agtgaggagt tatcagtgat agaaattgct aattataaag attaattatt
     2101 cactttaatc ttcgaatgaa ctttcatatt gtgcacaaaa tcatcgaaga ggtatcgcgc
     2161 atcgtacgga ccgggatttg cttcggggtg gtattgcacg ctgaaaacgg gtttcccttt
     2221 aagtcgaatt cctgcaaggg ttccatcgtt gaggtgcaca tgtgtcattt caatatcgct
     2281 tctattggaa agcgcttctt tatccacaac aaaaccatgg ttctgagagg tgatctcact
     2341 ttttccggtg atgaggtttt taatcgggtg attgattccg cggtgaccgt tatgcatttt
     2401 tttggtgggt aatccctgcg ataaggcaag aatctgatgg cccagacata taccgaaaat
     2461 aggaagtccg gttgcgacaa tggctttcac tttttcgatg gtatcgctca tcactcccgg
     2521 atcaccggga ccattactca gcataaaacc gttcggttca aaagcaagca tctcatcaag
     2581 tgaagcctgc atcgggaata cttttacata acatccacgc tccacaaggc aacgtacaat
     2641 gtttttctta acaccaagat cgagtaatgc aactttaaaa ggtgatgcag catctccgga
     2701 ggtgaatgct tctttcgtgc ttacccgaga agaaagttca agtccatcca ttgacggaac
     2761 agtcttcagt ttttgtttga gtatttccgg atccagaatt tcggatgaaa taatggcgtt
     2821 cattgcgccg gcgtttctga tatggcgcac aagtgcacgc gtatctacct cgcagattcc
     2881 aacaatatta tcccgctcga aataggtctg aagggaactt tctcctgaag gtctggagaa
     2941 attcactgag aattttttac agacaagtcc tgagatcttt acggaatccg attccacctc
     3001 atccttgtga acaccgtagt tgccaatatg cggtgaagcc ataacaacta tttgtccgag
     3061 ataggagggg tcggtaaaaa tttcctgata tccggtcatt cccgtattaa aggcaatctc
     3121 ccctgtggta gtgccaatga tcccggctgc tttgccgtgg aaaacagtgc cgtcttcaag
     3181 aacgagaagg gcggacggct tcacttggta tttcatggca caaatataaa tggaggaaag
     3241 caaaagggag ttcagaatgt tgctgtggtt ttgaacaaaa aatgcgggtt attcacgaag
     3301 acagaccggt ttatacccca tttgactccc atattgccat gaaggcaacc tgaatgattc
     3361 ctttttcgtc attataatcg agtcttgatt ccgttaatca cccagggcgg ttctttttcc
     3421 gctttgtgaa aaccattgaa atcagtatat ttacaaggat gcgacatttg tttacaatag
     3481 tgctggtttt catgcttttg gcgccttctt ttggtcacga gcatgggaat gatgagcgtt
     3541 attaccgctt cctgcctaat cagggacaat gggagcatgc aatcaggtac aaggcaaaaa
     3601 ttaacagcgg ggcattgttt ttactggaag attcctgggt ttttcatttt caggataatt
     3661 ctgttttgct aaaggctcat ttgggagaac ctgtaaagcc ggaggatgac aggatttttg
     3721 gtcagtctta caaagtgaaa tttatcggtg ccaaaaccaa aggttttgag cagaaggaaa
     3781 aaggtgagga gtattataat tttttcctgg gaaacgataa agataaatgg gcatcaaaag
     3841 tttttcccta caaacacatt acctacaaca atatttatca gggaatcgat cttcgcttat
     3901 actcaaaaga tgagcaatta aaatacgatt ggataattgc tcccggatca gacccttcac
     3961 ttattgttca ggaatatgaa ggagtagaaa agatccgaat taccaccatt ggagatctgg
     4021 aaatcaaaac aactgtagga accgtaatcg aaaaagcccc gaaagctttt cagatcataa
     4081 gaggaaagaa agttgaagtt ccctgcaggt ttacgcttaa ggggaaacaa ctgagctacg
     4141 aattccccaa tgggtacgac cgttcttttg aattgattat tgatccggtt cttgtttttg
     4201 gtagttatac aggctcaaca gccgataatt tcggaatgac agcaacctat tcttcgcaag
     4261 gccatttgtt caccggggga atggtatata atatcggtta tccaacaact ttgggagcgt
     4321 attccgtaac aagtaatgtg aacaattcag tatatggagt aacggatatt gtcattacca
     4381 agttttctcc caacggaacc agtctggtct attcaaccta tctgggcggc ggaactacga
     4441 atcagggaac agaaacggtt cacagtttaa tttgcaatgc caacgatgaa ttgtttttgt
     4501 ttggagtaac cagttctacc gattttccta caacagcagg agcctatgat cagactttca
     4561 atggagggag ttttattggt ttttaccaga atggttccta cttctacaat gcagggactg
     4621 atatttttgt ttccaaactt tctttcgacg gtgccaattt gcttgcatcc acctacatcg
     4681 gcggaacagg aaatgatggt gtaaattata accagaattt ccagcaaggt gtaaactggg
     4741 tttccaatta cgattccctg caaaccaatt acggagatca gtttcgcggt gaaattatgg
     4801 tggatgatct tgggaattgc tatgtggctt ccatgacaaa atcttcggat ttcccaattg
     4861 taaatgcgta tcaaagtacc tccggcggac aacaggatgg tgttgtgttt aaactcgatc
     4921 agaacctgag taatcttcaa ttcagcacct atatcggcgg aagcaataaa gatgcttgct
     4981 atggcataaa agtagatgca gcatacaatg tttttgtaac cggaggaacg gcttcagcga
     5041 attttccctc aacgccgggt acattgcata ccacctatca gggtgggaaa acggatgggt
     5101 atgtgatcaa actttcgcct actggaaata cattacttgc ttcaaccttt attggaacca
     5161 atgcctacga tcagacattt tttattgaac tcgatcgctt tgggtcggtt tacgtgttgg
     5221 gacaaacatc cagcccggct ggaatgcctc ctgttaatgt gaattattcg aatgccaatt
     5281 cagggcagtt cattgtaaaa atgaataatg cattaaacac attggaatat cgtaccttat
     5341 ttggaaatgg cagtggtaat ttcaatattt cgccatctgc ttttcttgtt gatgtatgtg
     5401 gaaatgtata tgtttccggt tggggagcaa atatcctgca gaacaatccg ctaacaggaa
     5461 tgccagtcac tgccaacgca tttcaacccg gaagcggaga tggatttaat ttttacctca
     5521 ttgttttctc ccgaaatatt gataatctac tttacgctac ctatttcggg ggtaatcaaa
     5581 gtcgcgaaca tgtcgatgga ggtacaagtc gttttgatgc caatggtgta gtttaccaat
     5641 cggtttgtgc aggatgtggt agtaatgatg attttccgac aacgcccggt gcatggtcaa
     5701 atataaattt gtcaaccaat tgcaacaacg gtgttttcaa atacgatatg gaaattgaac
     5761 cggaagcaga ttttgtaaca gataaactcg aaggctgttc accacttact attcattttg
     5821 ataataccag tccattgtat tcagaattcc tatgggattt tggcggtggc gataccacgt
     5881 ctacaattgt caatccggtc tatactttta ccactcccgg aacatacaca gtatatctta
     5941 ttgttcgcga ttccatttgt cttttgctcg atactgcaga aaaagtaatc acagtttatc
     6001 ctcccttaca attgtttact tccgatactg taatctgctc tccgcaaacg gttgcactgc
     6061 tggcaaattc gaatggtacg tcaacgaatt tttactggtc aagcaacggg aatttttccg
     6121 acacattgaa tgcgccgata acggatagta cattgagtgt atttgtttcc ggtactacca
     6181 attattacgt caatgttgca aatacctggt gttcttatac cgatactgta actgtatttg
     6241 ttcctgatct tgatatctca atcacgtcaa taaacggaat ttgtacagga gatacggttg
     6301 cattaactgt taccaacaat acgccgttgt ttccattaag ctacaattgg tttcccgatt
     6361 cacttattct atccggagat ggatcttcaa ctgtattggt aagtccggat tatgataact
     6421 ggatttacgt aacgggaacc actgcagaag gatgtaccat tacggattca acatttatta
     6481 acgtagatgg accaccaacg ggattggttt cagctactgc tgatcaatac gcaattcttg
     6541 caggggcaag caccgtttta catgcccaac ctaatgggta ttcgtatacc tggacaccag
     6601 cttttacact cgataatccc aattcacaac acccaactgc tacacctaca gaaacaacca
     6661 tttatactgt tacaatttcc aatggaggtt gttcactaac agatactgtc agggtgcgta
     6721 ttgtagaaat tttctgtggt gagccgaata tttatattcc caacgcattt acacccaatg
     6781 gcgatggtga aaatgatttt ctttttgttc gtggaaacaa tatcacaaaa ctagttttca
     6841 gggtgtacga tcgttgggga gaaaaagtat ttgaaac
//
LOCUS       DAOMYG010000129         6861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3755_length_6861_cov_9.838525, whole genome shotgun sequence.
ACCESSION   DAOMYG010000129 DAOMYG010000000
VERSION     DAOMYG010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3755_length_6861_cov_9.838525"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PK610_05575"
     CDS             complement(<1..663)
                     /locus_tag="PK610_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRJ35434.1"
                     /translation="MLSIKEVQAPIHSEIAEFEIRFREAMKSRTVLLDKITHYIVKRK
                     GKQMRPMFVFLSAKTCGEISDSAYTGAGLIELLHTATLVHDDVVDDSNERRGFFSLNA
                     LWKNKIAVLVGDYLLSKGLLLSIDKGEFRLLQIVSNAVREMSEGELLQIEKARKLNIT
                     EEIYYEIIRQKTASLVAACCACGAASTKCDEAAIDKMYQFGEHVGMAFQIKDDIFDFD
                     LKG"
     gene            complement(707..2311)
                     /locus_tag="PK610_05580"
     CDS             complement(707..2311)
                     /locus_tag="PK610_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35435.1"
                     /translation="MRPIVFFLLFLVLCSNSGLTQLVVQWSEEFKFDRRVSIAGMMSD
                     PNFFYVLKRNTSGKGNPAPYFLEQFDASSMAIRRQMEIRFPVAPHSPGVVFHDLLSVD
                     GKLWLFLQGTNKRGRNGRLYVAPVDMDQAKIGEAILIDSFANPVASADFRIEESWNGK
                     TILVMHNDPFDIKYNSEKFYYKVYNNNMNLLWKKEIEMPFRDRMFKVTHHLLDHKGNV
                     HLVSAVHQEREKGDQRDNAVSNNKYHLISYYHEQNLVKEFEISLGEKWISALTFDMAP
                     DGQLVLAGFYSNAANYSISGTFYLRIDPETRSVVQSNMKAFDKNFLLEFLPERKVKKG
                     IELSDFYFDHLVVREDGSAMLIAEQYYMQVVYTYNDPYMNGMYGWGYPYGGYSPYYRS
                     NYNYQYHYNDIIVVSIDPEGNIDWTKKIPKRQMSSNDGGYYSSYTLASNERMVYVLFN
                     DNPRNSAEFRKGTAEPYAMTKPSKSQAMMVTIDREGKFVYNALFAQKESGLILRPKLC
                     YQYSADRLILYSQKGSRYRFGAIGLP"
     gene            complement(2429..3490)
                     /gene="rlmN"
                     /locus_tag="PK610_05585"
     CDS             complement(2429..3490)
                     /gene="rlmN"
                     /locus_tag="PK610_05585"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRJ35436.1"
                     /translation="METVKTDIRSLSFEELKSRFEEMGEKTFRAKQVYEWLWKKSIHD
                     FDGMTNLSLELRNKLKERFVIYNITLSDRQVSADRTIKCAFSLHDGKVVEGVLIPTNS
                     RMTACISSQVGCSLTCAFCATGKLKRMRNLEASEIYDQVVTLKNEAENTYGIPLSNIV
                     YMGMGEPLLNYRSTLESVSKITSPEGLGMSPQRITVSTAGIAKMIMKLGDDGVKFNLA
                     LSLHAANDEKRNKIMPINEQNTLDALAEALRYFYDKTGTRVTYEYIIFKEFNDSLQDA
                     RELAEFCKHVPCKVNIIEYNPIDDGEYRQADSEKVDAFARYLESRNIVVNIRRSRGKD
                     IDAACGQLANKNKAAYKED"
     gene            complement(3550..>6861)
                     /locus_tag="PK610_05590"
     CDS             complement(3550..>6861)
                     /locus_tag="PK610_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRJ35437.1"
                     /translation="PYPGAGPKVIEDKITRKIEKEVNTIRGVDKIKSTSIFGYSTIIV
                     EFNFDVTPEQGLRKVKDAVDKARGDKDFPRDLPAEPNVFEMNFSEFPIMNINLSGNYS
                     VDELTEFGEFLEDEIEKIEEISKVEIRGVQKKQVSISINKFEAESRMVSFGDIENAIT
                     SENMTMSGGEVKMSGIARDVRVEGEFASLEEIRNIIVKRDYYNIVRLRDVAEVTFGDE
                     DTTSFARQNMKPVVMLDVIKRSGTNLLEASDKIFDVVNEAKAKGRIPSDLELTYTNDM
                     SFKTRDQVSSLENNIISGVILVVLILQLFLGLRSALFVGVAIPMSMFLSFIILNVMGV
                     TLNIMVLFSLVLALGMLVDNGIVVIENVYRLTNEGYSLFDAVKYGVGEIALPIISSTA
                     TTVAAFIPLALWPGLMGEFMQYLPLTLMIVLSSSLFVALVINPALAILLMKTVEERPA
                     LAKVIRNAMIGFGLGLLLLLLGKFTLGNLALTFGALVLINYYFLDAATNFMQNKLLPT
                     LERRYRAFLEYALVKRPLTIFLSTIAMLFLSFMLVGVFQPKVLFFPENEPAYVNVFVE
                     LPVGTDIRLTNEITKSAELIIDSTLAKYDDVIMLAEKPLGDTVVVDSFRLVSSMIAQV
                     GEGTSDPMEGPSMGNTPNKARITISFAEAPYRQGHRTSDIKREIEQALEGRFPADVKI
                     VVAKDAAGPPQQPPINIELYGDDYDSLIVEAENMRTFLDLQNVPGVDELKLDVEIGKP
                     ELPIEVDREVARRLDVSTAQVAMTIRTALFGKDVSTYKIGDEDYDINVRYKEDYRHDL
                     QALLDQKITFRDMLSGKLVQVPIRTVILPPKNKSSYSAVKHKNTDRVVTIFSNVKEGE
                     NPTEIVNQLKEKLTSYNVGNNVSWKFTGQQEDMEKEMKFLSGALMIAVFMVFLIIVAQ
                     FNSISSPAIIMTSVILSLIGVLLGLIIFQMDFVIIMTMIGIISLAGVVVNNAIVLIDY
                     TILLINRRREELGIGEDENLPMDEIVDCIAKAGGTRLRPVMLTAITTLLGLIPLAVGI
                     NVNFITLYSEYDAQFFIGGDNVMFFGPLSWTVIFGLTFATFLTLVITPIVFLLIHRLK
                     VRLYKRTKWKLLKTY"
BASE COUNT         2026 a         1584 c         1289 g         1962 t
ORIGIN      
        1 gcctttcaga tcaaaatcga aaatgtcgtc tttgatctga aaggccattc caacgtgctc
       61 gccaaattgg tacattttat caatagcagc ttcatcacat ttggtagagg ccgccccgca
      121 agcacaacaa gcagcaacta aagacgctgt tttctggcgg ataatttcat aataaatctc
      181 ctctgtaata ttaagctttc ttgctttttc aatctgaagc aattctcctt cgctcatttc
      241 acgaaccgca ttgcttacta tttgcaggag tcgaaattct cctttatcga ttgaaagcaa
      301 taatcccttc gagaggagat aatcacctac caatacagct attttatttt tccataaagc
      361 attcagtgaa aagaatcccc gccgctcatt cgaatcgtca accacatcat cgtgcaccaa
      421 tgttgcagtg tgcaataatt caataagtcc ggccccggta tatgcgctat cactgatttc
      481 cccacatgtc tttgcgctaa ggaatacaaa catcggacgc atctgttttc cctttcgctt
      541 cacgatgtaa tgcgtgatct tatcaagcaa aaccgtacga cttttcatcg cttcacgaaa
      601 acggatttca aactcggcta tttccgagtg aatgggtgct tgtacttcct ttatgctaag
      661 catatgttgc gaagataagg gggaattttt aatgaaagat aaatttttag ggcaagccaa
      721 tcgctccgaa tcggtagcgg cttcctttct gactgtagag tatcaggcga tcagcagagt
      781 actgataaca gagcttggga cgaagaatta atcccgactc cttttgagca aacaaggcat
      841 tgtaaacaaa cttcccttct ctatcgatgg taaccatcat ggcctgactc ttggagggtt
      901 ttgtcattgc ataaggctca gctgttccct tacggaattc cgcagagttt ctgggattat
      961 cgttaaacaa aacatatacc atgcgttcat tgctcgccaa ggtgtaagat gaataatacc
     1021 caccgtcatt ggaagacatt tgtcgttttg gaattttttt tgtccaatca atatttcctt
     1081 cgggatcaat acttaccaca atgatgtcgt tgtaatggta ctgataattg taattgctgc
     1141 ggtaataagg ggaatatccg ccatagggat atccccatcc gtacatgcca ttcatgtatg
     1201 gatcgttata cgtgtaaacc acttgcatgt aatattgttc cgcgatcagc attgcgctac
     1261 cgtcttcacg cacaacgagg tgatcaaaat aaaaatcaga aagttcaata ccctttttca
     1321 ctttacgttc gggtaaaaat tcgagcagaa aatttttatc aaacgctttc atgttcgatt
     1381 gcactacact tcgcgtttca ggatcgatcc gcaaatagaa tgttccgctg atggaataat
     1441 tcgctgcatt actgtaaaaa ccagcaagca ctaactgtcc atcaggagcc atatcaaatg
     1501 ttaaggccga gatccatttt tctcccaatg aaatctcaaa ttcctttacc agattttgtt
     1561 cgtgataata agaaatcaaa tggtacttgt tattggatac tgcattatcg cgctggtcac
     1621 ctttttcccg ttcctgatgc acagcgctga ctaaatgtac atttccttta tgatccagta
     1681 aatgatgcgt taccttgaac atgcgatcac gaaaaggcat ttcgatttcc tttttccaaa
     1741 gcaaattcat attgttattg taaactttgt aataaaattt ttcgctgttg tatttaatat
     1801 cgaatggatc attatgcatc accagaatgg tttttccgtt ccatgattct tcaatacgga
     1861 aatcggcaga tgcaacaggg tttgcaaacg aatctattag aatagcctca ccaatttttg
     1921 cctgatccat atcgaccggg gctacataca atctaccgtt tctcccccgt ttgtttgtgc
     1981 cttgtaagaa aagccagagc ttcccatcaa cagaaagcaa atcatgaaaa accacaccgg
     2041 gagaatgtgg tgctacggga aaacgaattt ccatctgacg acggatggcc atgcttgatg
     2101 catcgaactg ctcaagaaaa tagggggcag ggtttccttt accggaagta tttcgtttta
     2161 acacataaaa gaaattcgga tcactcatca ttcccgctat acttaccctt cgatcgaact
     2221 taaactcttc actccattgc acaacaagct gagtcaaccc gctattgctg cacaacacta
     2281 aaaaaagtag gaaaaaaacg attggtctca tttgtgtact ccgcagtgct taaagatacg
     2341 aatcaggtgt attgacggtt tcaataaatc aaaattatat cctgcttgtg ctttcaatta
     2401 cctccagctc aaacagtatt ttcgaaaatc aatcctcttt gtaggctgct ttattcttat
     2461 ttgccagctg accacatgca gcatcaatat ccttaccgcg gcttctgcgg atgtttacaa
     2521 caatgttgcg cgattcgaga tagcgggcaa aggcatccac tttttcggaa tctgcctgac
     2581 ggtattctcc gtcatcgatt ggattgtatt caataatatt caccttgcag ggaacatgct
     2641 tgcaaaactc tgcgagctca cgggcatcct gtaaagagtc attaaactct ttgaaaatga
     2701 tatactcata agtgacacgc gtacccgttt tgtcgtaaaa ataacgcagg gcctcggcaa
     2761 gcgcatccag cgtattttgc tcattgatcg gcatgatctt gttccgcttc tcatcattgg
     2821 cagcatgcag ggaaagagca agattaaact ttacaccatc atcacccaac ttcataatca
     2881 tcttcgcaat accagctgta gaaaccgtta tccgctgagg agacattccc aaaccttccg
     2941 gagaagtgat tttgctgacc gactcaagtg tgctacggta gttcaacaaa ggctctccca
     3001 ttcccatata aacaatattg ctaagcggaa tgccatatgt attctccgcc tcgttcttaa
     3061 gtgttacaac ctgatcatat atttcactgg cttcaaggtt acgcattcgt ttcaacttac
     3121 ctgttgcaca aaacgcgcag gtaagcgagc aaccaacctg cgatgaaata caagcggtca
     3181 ttctggaatt agtggggatg agcactcctt ctactacttt tccatcgtgc aaggagaatg
     3241 cacacttaat ggttcgatct gctgaaacct gacgatcgga taatgtgata ttataaataa
     3301 caaaccgttc cttcaacttg ttccttagct ccagcgacaa gttggtcatt ccatcgaaat
     3361 catgaatcga ctttttccac agccactcat aaacttgctt ggcacgaaaa gttttctctc
     3421 ccatttcttc aaaacgcgat ttcaactctt caaatgagag ggatcgaata tctgttttga
     3481 ccgtttccat aggacaaaga taaggtgatt aaacgacaat gcccgaattt atgaatccgg
     3541 gcattgtcat taataggttt taagcagttt ccatttcgtc cgcttgtaca gcctcacctt
     3601 taatcggtgt atcaacagga atacaattgg tgtaattacc agtgttaaaa aggttgcgaa
     3661 tgttaatccg aaaattacag tccagctcaa cggaccaaaa aacattacgt tatccccacc
     3721 aataaagaac tgtgcatcat attcggaata caaggttata aagttaacgt tgataccaac
     3781 cgcaagtgga atgagtccta acaaggtagt aattgccgta agcattacgg gacgaagtcg
     3841 ggtaccaccg gctttggcga tacaatctac aatttcatcc attggcaggt tttcatcttc
     3901 accaattccc agttcttcgc gtcgacgatt aatgagcaga atggtatagt cgattaaaac
     3961 gatagcgttg ttcaccacga ctcctgccag tgaaataata ccgatcatgg tcatgataat
     4021 cacaaaatcc atttggaaaa tgataagtcc aagcagaaca ccaatcaaac tcagaattac
     4081 ggatgtcatg ataattgccg gtgaactaat agaattaaac tgagccacaa taatgaggaa
     4141 aaccataaat acagcgatca tcaaagcacc ggaaagaaat ttcatttcct tttccatgtc
     4201 ttcctgctga ccggtaaatt tccagctcac gttatttcca acattgtagg atgttaactt
     4261 ctccttcaat tgattaacaa tctccgttgg attttctcct tcttttacgt ttgaaaaaat
     4321 ggtaacaacc ctatcggtgt ttttatgctt aactgccgaa taggaagatt tgtttttggg
     4381 tggaagaata actgttcgga taggaacctg taccagtttt ccgctcaaca tatcacgaaa
     4441 agtgattttc tgatcgagca aggcctggag gtcatgacga taatcttctt tgtatcgaac
     4501 attaatatca tagtcctcat cgccgatttt gtaagtggaa acatctttac caaacaatgc
     4561 tgtacgaatg gtcattgcaa cctgggctgt agaaacatcc aatcggcgcg caacttcacg
     4621 atcaacttct atgggtaatt caggttttcc gatttcaaca tccagcttca attcgtccac
     4681 acctggaaca ttctgcagat caagaaatgt ccgcatattc tcagcctcta ctatgagcga
     4741 atcgtaatca tcaccataaa gttcaatatt gattggcggc tgttgtggtg gacctgctgc
     4801 gtctttggca actacaattt ttacgtctgc gggaaaacgc ccttcgagcg cttgctcaat
     4861 ctcacgtttg atatctgaag tacgatgtcc ctgacgatag ggggcttctg caaatgaaat
     4921 ggtgatacgc gccttattgg gtgtattacc cattgaaggg ccttccatcg gatctgatgt
     4981 gccttctcca acctgagcga tcatcgaaga aaccaaccgg aacgaatcca ccaccacagt
     5041 atctcccagc ggcttttctg caagcataat cacatcatcg tatttcgcca atgttgaatc
     5101 gatgataagc tctgcacttt tggtaatctc gttggtaaga cgaatatccg ttcccacagg
     5161 aagttccaca aatacattta catatgcagg ttcgttttcc ggaaaaaaca aaactttagg
     5221 ctggaaaacc cccacaagca taaacgaaag gaacaacatt gcaatcgtgc tcaggaaaat
     5281 agtgagcggt cgctttacta atgcatactc caaaaacgca cggtaacgac gttccaatgt
     5341 aggcaagagc ttgttctgca taaagtttgt tgcagcatcc agaaagtaat aattgatcaa
     5401 taccaatgcg ccgaaagtaa gagcaaggtt accaagcgtg aactttccta aaagcagtaa
     5461 caataaacct aatccgaacc caatcattgc attgcgaatg acctttgcca atgcagggcg
     5521 ctcttctaca gttttcatca agagaattgc tagagcagga ttaataacca atgccacaaa
     5581 caacgaagaa ctcaatacga tcatgagtgt gagtggcaga tattgcatga actcacccat
     5641 aagaccgggc caaagggcaa gagggataaa cgcggcaacg gttgttgcag ttgaggagat
     5701 aattggtagc gcaatttccc ctaccccata ctttaccgca tcaaacaggg agtagccttc
     5761 gttggttaat cggtaaacat tttcaataac cactattccg ttatccacca gcattcccag
     5821 ggcgagtacc aatgagaaca ataccataat gttcaaggtt actcccatca catttaggat
     5881 aatgaaagaa aggaacatcg acataggaat tgcaacacca acaaataatg cgcttcgtaa
     5941 tcccaaaaac aattgtaaga tcagaacaac aagaataact ccggaaataa tattgttctc
     6001 cagactcgaa acctgatcgc gtgttttaaa cgacatatcg ttggtgtaag tcagctcaag
     6061 atctgaaggt attctgcctt ttgcttttgc ctcatttacc acatcaaaaa tcttgtccga
     6121 cgcctcaagc aaattggtgc cggaacgttt gattacatca agcatcacaa caggcttcat
     6181 gttttgtcgt gcaaatgatg tagtatcctc atcgccaaaa gttacttcag caacatcacg
     6241 cagacgaacg atgttgtaat aatcgcgttt cacaataata tttcggatct cctccaggct
     6301 ggcgaattca ccttcaacgc gaacatcacg ggcaataccc gacattttca cttcaccgcc
     6361 cgacatggtc atgttttcag aagtaatggc attttcaata tcaccaaaac tcaccatgcg
     6421 cgattctgct tcgaacttat tgatggaaat actaacctgc tttttctgca caccgcgaat
     6481 ttccaccttg gagatttctt caatcttttc aatttcgtct tccagaaact ctccaaactc
     6541 tgtcaattca tcaaccgaat aatttccgga aagattgata ttcataattg ggaattccga
     6601 aaaattcatc tcaaaaacat tcggctctgc tggaaggtcg cgagggaaat ctttatcacc
     6661 acgggctttg tctaccgcat cttttacctt ccgtaatccc tgctcgggag taacatcgaa
     6721 attgaattca acgataatag ttgaataacc gaaaatgctg gttgatttga ttttgtccac
     6781 tccacgaatg gtattcactt ccttctcgat cttacgtgtg atcttgtctt caatcacttt
     6841 cggaccggct ccgggataag g
//
LOCUS       DAOMYG010000130         6837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3771_length_6837_cov_11.200531, whole genome shotgun sequence.
ACCESSION   DAOMYG010000130 DAOMYG010000000
VERSION     DAOMYG010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6837
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3771_length_6837_cov_11.200531"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..2193)
                     /gene="rnr"
                     /locus_tag="PK610_05595"
     CDS             complement(43..2193)
                     /gene="rnr"
                     /locus_tag="PK610_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRJ35438.1"
                     /translation="MAKNNSHSSSKKLEQFLRKEILIHMTENPDRSFNYKQLAKAIGV
                     DDNREKQMLMDILDDLREDGKLKEVDRGRYVIKQDRKYLTGTIEINSRGNGYLLSGQE
                     GIQDVFINKNDLNRSLNGDTVKVLLHPSRRDKQPEGEVVEIIQRSTDTFVGRIELSKN
                     FAFLIPDSQRMHVDIFIPLEKLNGARHMDKAIVQLGDWPSHATNPFGKVIEVLGKAGE
                     NETEMHAILAEFGLPYHYPKDVENYANKLDPAISSAEVSKRRDFRSITTFTIDPEDAK
                     DFDDALSIQFLREDKGKKIYEIGVHIADVSHYVVPESILDKEALNRATSVYLVDRVVP
                     MLPEILSNQICSLRPNEDKLTFSVVFEIDEDANVRKEWFGRTVIHSDRRFTYEEAQKI
                     IEGNDGDLMNEIRIMDKMAKIIRDKRMANGALGLESVEVKFRLDDKGKPIGVYTKVSR
                     DANKLIEEFMLLANKAVAKYIGEPDGKKKIYPMVYRIHDEPKEEKLKELSTFVSLLGY
                     KLKSYDSRRLPQAIAELLKDIKDPQVAETIQTYTIRSMAKAIYDVNNIGHYGLAFDYY
                     THFTSPIRRYPDLIVHRMLQHFLDGDKGPSKSQLELWCKHSSAQEKKASDAERASIKY
                     KQVEYMKENIGKIFDGVVSGVAEWGIFVEVAESKCEGMISLRSIRDDMYVYDQKALCV
                     RGTKTKKTFRLGTKVKIKVKGADMMKRQIDFELM"
     gene            2287..3654
                     /locus_tag="PK610_05600"
     CDS             2287..3654
                     /locus_tag="PK610_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35439.1"
                     /translation="MKGKSLFLLIGELNKAERHQLLNTCKRSGDKRHLVLYQLMKKHT
                     GTKDTFEDVLFSVAKPLFGGEKDEKEKDKTMRRFIDFSLKEIEQIKLRNFLQEDQLLR
                     HFLLSRIYESKGDASIERRYLERTGELSDQLHDRFMKAYYVDRMVDHTSRTHTKKEMK
                     ILQELLAEKNALIQKNYHAELARIYDLLSVLRFEDKELAEGLKGLVLEDDEVDMLIAL
                     SGGSPEEIDYLVARARFAFFEPAKFTAFIQRSLASLKKIRDGREKERLNRKISFLFCQ
                     HAFHFGEAPGKLLEYAQALSSSLNGLELFYFHLARILDLHSRKKPLPEPKELSKIPME
                     PENQFRIEFLQALVYFLKGEHSAAVKLLNQVSYVSNFQVSTWSRLMEFRLHLNKGNLN
                     LCDSLSGRLLRHFQGNKGKEFTLSSSKELFALFQDELKVKSKKPGKKSATELCALHKA
                     IVLQR"
     gene            3705..4514
                     /locus_tag="PK610_05605"
     CDS             3705..4514
                     /locus_tag="PK610_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ35440.1"
                     /translation="MMRLLFLVLFVLSGLLAHSCTIDSYVLTRPTCLYSADGQLQIQA
                     SGAVGITYSIDGGLTFSASNTFSNLAPGTYFIVIQSPLPCSVSDTVYIAPQSQLSAGG
                     TVSTNTIFPGESVVFSSYSHNATSTLFDFGDGSDTTGLTQVVHSYNQIGAFTPMLIVS
                     DGICFDTITFSVTVAGESSLYVPNVFTPNNDDVNDLFFPRAFGMRSLECTIMNRYGEI
                     VHSWIGTKGFWDGYTFPAGVACSEGTYFYWIKAEGLDGKSYNVNGSITLLR"
     gene            complement(4583..4909)
                     /locus_tag="PK610_05610"
     CDS             complement(4583..4909)
                     /locus_tag="PK610_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35441.1"
                     /translation="MERKEAERLIDSITQDYLKNGSSEKVINDLKTLRNFFIEIKDPT
                     LTKIIRLTYEYLESNEDFDIVLEDFEEDSDVNSFEYLMQLVANYDNEYNREELNEYKF
                     RMMEAL"
     gene            complement(4925..6478)
                     /locus_tag="PK610_05615"
     CDS             complement(4925..6478)
                     /locus_tag="PK610_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRJ35442.1"
                     /translation="MSAVMTDFGIAETLKVLGIKDKNSGASTGNYWFATRGEEIVSYS
                     PVNGEKIASVHAATEAEYEALILKANEAFKVWRKVPAPKRGEIVRQMGEELRKYKNEL
                     GKLVSYEMGKSYQEGLGEVQEMIDICDFAVGLSRQLHGLTMHSERPNHRMYEQYHPLG
                     IVGIISAFNFPVAVWSWNSMLAWVCGDVCIWKPSEKTPLCAIACQNIIADVLKRNEVP
                     EGVSCVIIGDHTVGELMSNDKRVPLVSATGSTRMGKAVGKAVGERLGRSLLELGGNNA
                     IIITENADIDIALLAVVFGAVGTAGQRCTTTRRLIIHESMYETVKNKIKSAYNQLRIG
                     NPLDQNNHVGPLIDKQAVEMYLHALKEAEKEGAKVVVEGGVLTGDGFESGCYVKPAVY
                     EAKNSFKIVQEETFAPILYIMSYKTLDEAIELQNGVVQGLSSSIMTLNMREAEQFLST
                     AGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKVYMRRQTNTINYGNQLP
                     LAQGIKFDI"
     gene            complement(6568..>6837)
                     /locus_tag="PK610_05620"
     CDS             complement(6568..>6837)
                     /locus_tag="PK610_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRJ35443.1"
                     /translation="FGSIGGMFPKEWDWQQFWLLTAFISIILAFMNLLPIPALDGGHV
                     LFLLYEMVRGKAPNQRFLEIAQTVGMLLLLGLILYANLNDVLKLF"
BASE COUNT         1773 a         1483 c         1457 g         2124 t
ORIGIN      
        1 ccgtgtgatt tgaagaattt cacgctgttt tgttatgatt gattacatca gttcaaaatc
       61 gatctgacgc ttcatcatat ccgcgccttt taccttgatc tttacttttg tccccaagcg
      121 gaatgtcttt tttgtttttg ttccgcgcac acaaagtgct ttctggtcgt aaacatacat
      181 gtcgtcccga atgcttctta atgagatcat cccttcgcac ttactttctg ctacctcaac
      241 aaaaattccc cattctgcca cacccgaaac tactccgtcg aatattttgc caatattctc
      301 tttcatgtac tctacctgtt tgtacttgat cgaagcccgt tctgcatcgc ttgccttctt
      361 ttcctgggca gaggagtgtt tacaccataa ctccaattgg gattttgaag gccctttatc
      421 gccatccagg aagtgttgaa gcattcggtg tacaataagg tcgggataac gccgtatggg
      481 tgatgtaaag tgggtgtaat agtcgaacgc caggccgtag tgaccaatat tgtttacatc
      541 gtatatagcc tttgccattg aacggatggt ataggtttgt atggtttcgg caacctgtgg
      601 gtctttaatg tcttttagta attctgcaat cgcctgtggc agtcttcgcg aatcgtacga
      661 ttttagctta taacccaaga gcgaaacaaa ggtggagagc tccttcagtt tttcttcctt
      721 gggctcgtcg tgaattctgt aaaccattgg ataaatcttc ttttttccgt ccggttcacc
      781 aatgtatttt gcaacggctt tattggcaag caacataaat tcttcgatca gcttgtttgc
      841 gtcacgcgat actttggtat atacgccgat tggttttcct ttatcatcca ggcggaattt
      901 tacctcaaca ctttccagtc cgagagcgcc atttgccatt cttttatcgc gaatgatttt
      961 ggccattttg tccatgatcc ggatttcatt catcagatca ccgtcgttgc cttcaattat
     1021 tttttgcgct tcttcataag taaatctgcg gtcggaatga atgactgttc tcccaaacca
     1081 ttctttgcgc acattcgcat cttcatctat ttcgaatacc acagaaaaag tgagcttgtc
     1141 ttcgttcgga cgcaaggagc agatctggtt ggaaagtatt tccggtaaca ttggcaccac
     1201 ccggtctacc aggtaaacag atgtggctct gttcaaggct tctttatcaa gtatactttc
     1261 gggtacaacg taatgcgaaa catcggctat atgaacccct atttcataga tctttttacc
     1321 cttgtcttca cgcaggaact gaatcgataa ggcatcgtcg aaatcttttg cgtcttcagg
     1381 atctattgtg aatgtggtta tcgatcgaaa atcgcgacgt ttggacactt ctgccgaact
     1441 tatggcgggg tcaagtttat tggcgtaatt ttcaacgtcc tttggataat gataaggtaa
     1501 tccgaactct gcaaggatgg catgcatttc tgtttcattt tccccggcct tgcctaaaac
     1561 ctctatcact tttccaaagg gattggtagc gtgtgagggc caatcgccaa gctgaacgat
     1621 tgctttgtcc atgtgcctgg cgccgttcaa tttttccaac ggaatgaaaa tatcaacgtg
     1681 catgcgttgc gaatcaggaa taagaaatgc aaagttttta cttaactcga ttctgccaac
     1741 aaatgtatcg gtagatcgct gtattatttc taccacctct ccttccggtt gtttatctct
     1801 gcgggagggg tgcagcaaaa cttttaccgt atcaccattc aatgaccggt taagatcgtt
     1861 tttattgata aagacatcct gaatcccttc ctggcctgat aataagtaac cattaccccg
     1921 actgttgatt tcaatggttc ctgttaggta tttacggtct tgtttgatca catatctacc
     1981 cctgtccact tctttgagct tgccgtcttc tctcaggtcg tcgagaatgt ccatgagcat
     2041 ttgcttctcc ctgttgtcgt ccactccaat tgctttggca agttgcttgt agttgaacga
     2101 ccggtcgggg ttttcggtca tgtgaatcag gatttcctta cgcagaaact gttcaagctt
     2161 tttagaactg gaatgtgaat tattttttgc catacagcgg tttggggtct gttaaaggta
     2221 gtactttgat gcactgtatt ggttcagtca gcttttgttt tacgacataa tagtgttaac
     2281 aagtccatga aaggcaaatc actttttctt ctgatcggag agcttaataa ggcggaacga
     2341 catcaattgc tcaacacgtg taaacgctcg ggcgataaac ggcatttggt tttgtaccag
     2401 ttgatgaaaa agcataccgg aacaaaagac acttttgaag atgttctgtt ttcggtagca
     2461 aaaccattgt ttggaggtga gaaagacgaa aaggaaaaag ataaaaccat gcggcggttt
     2521 atcgattttt ccctaaagga aattgaacaa ataaaactgc ggaatttttt gcaggaagat
     2581 caattgttgc gccatttcct tctttcccga atttacgaaa gcaaaggcga tgcctctata
     2641 gagcggcgtt acctggaacg aacaggcgag ctttcggatc agttgcacga tcgttttatg
     2701 aaagcgtatt atgtagatcg catggtagat catacttcgc ggacacatac caaaaaggaa
     2761 atgaaaattc tgcaggaact actcgcggaa aaaaatgcac tgatccaaaa aaattaccat
     2821 gccgagcttg cgcgtattta tgacttgttg tcggtgttgc gttttgagga caaagaacta
     2881 gctgaaggtt taaagggact tgttcttgaa gatgatgaag tggatatgtt aattgcactt
     2941 tccggaggta gtcctgaaga aattgattat ttggtagcca gagcgcgttt tgcttttttc
     3001 gagccggcaa aattcacggc ctttattcaa cgttcacttg cttcactaaa aaagatccgt
     3061 gacggaagag aaaaggaacg gttaaaccgt aagatttcat ttcttttttg tcagcacgct
     3121 tttcattttg gtgaggcccc gggtaaactg cttgagtatg cacaagcgct ttcctcttct
     3181 ttaaatggat tggagttatt ttattttcat ctggcacgta tacttgatct tcattccaga
     3241 aagaaacccc ttcccgagcc aaaagagctt tccaaaatac caatggagcc ggagaatcag
     3301 ttccgcatcg agtttttgca ggctctggtt tattttctga aaggagaaca ttctgcggct
     3361 gttaaattgc tgaatcaggt gtcttatgtt tccaactttc aggtttctac atggtcaagg
     3421 ttaatggagt tcaggctgca cctcaacaag ggtaacctga acctgtgcga tagcctttcc
     3481 ggacgattgc tgcgtcattt tcaaggcaat aaagggaagg aatttaccct ttcttcaagc
     3541 aaggagctgt ttgcattatt tcaggatgag ctgaaagtaa aaagtaaaaa gccgggtaaa
     3601 aagtcggcaa ccgaactttg tgctttgcat aaggctattg ttttacaaag gtaaagcgac
     3661 aaaaaggctt atatttacgt cttaccgcag aaagcgacag aagaatgatg cgattgctgt
     3721 ttttggtcct gtttgttttg tccggattgt tagctcattc ctgtacaatc gacagctatg
     3781 tgctaaccag gcctacctgt ttgtattctg ctgacggaca attgcagatt caggcatcag
     3841 gtgcggtggg tattacttat agcatagatg gcgggctaac gttttcggct tctaacacat
     3901 tcagtaatct tgcacctgga acttatttta ttgttatcca atcgccatta ccttgttcgg
     3961 ttagcgatac agtttacatt gctcctcaga gtcagctttc tgctggagga acggtttcta
     4021 caaataccat atttcctgga gagtcggttg ttttttccag ctattcgcat aatgcaacaa
     4081 gcacactatt tgattttggt gatggcagcg acacaacggg attaactcag gttgtgcatt
     4141 cctataacca aattggagcc tttacgccaa tgttgattgt tagtgacgga atttgttttg
     4201 atacgattac cttttcagtt actgttgcag gagagtcgtc cttgtatgtt cctaacgtgt
     4261 ttacaccgaa caatgatgat gtaaatgatt tgttttttcc tcgtgctttt ggcatgcgtt
     4321 cattggagtg cactattatg aaccgctatg gagaaattgt gcattcatgg ataggaacaa
     4381 aaggtttttg ggatggatac acctttccgg ccggcgttgc ttgttcggag gggacctatt
     4441 tttattggat caaagccgaa ggattagatg gtaaatccta taacgtaaac gggtcgatca
     4501 cgctcctgcg ttagtggggc atatctctat aaacagcaaa gggccctttt cggggccctt
     4561 tgttttttat attgtgtttc gcttaaagcg cttccatcat acggaatttg tattcattga
     4621 gttcttcgcg gttgtattcg ttatcgtaat tggcaacgag ctgcatcagg tactcaaagc
     4681 tgtttacatc cgaatcttct tcaaagtctt caagaacaat atcgaaatct tcattgcttt
     4741 caaggtattc gtaagttaag cgaataattt ttgtaagcgt agggtctttt atctcaataa
     4801 agaagttgcg aagtgttttt agatcattaa tgaccttttc gcttgaacca ttcttaaggt
     4861 agtcctgcgt gatgctgtct attagacgct cggcttcttt tctttccatt gccggttttg
     4921 ttttttagat atcgaatttt attccttgtg ccaaaggtaa ttgatttccg taattaatcg
     4981 tgttggtttg gcgacgcata taaaccttcc atgcatctga acccgattca cgacctccac
     5041 cggtctcttt ttcaccacca aaagcaccac caatctcagc tccggaggtt ccgatattta
     5101 cattggcaat gccacagtct gaacctgctg tagataaaaa ctgttcagct tcgcgcatgt
     5161 ttagggtcat gatcgaagaa gatagaccct gaacaacgcc attttgaagc tctatagcct
     5221 catcaagcgt tttgtacgac atgatgtata gaatcggggc aaatgtttct tcctgaacaa
     5281 ttttaaagct gtttttcgct tcgtaaacag cgggcttaac ataacagcca ctttcaaatc
     5341 catcgccggt gagaacgccg ccttcaacta caactttggc tccttctttt tctgcttctt
     5401 ttaaagcatg gagatacatt tctacagcct gcttgtcgat gagaggacca acgtgattgt
     5461 tttggtcgag cggattaccg atacgtaatt ggttgtaggc tgatttgatt ttgtttttta
     5521 ccgtctcata catcgattca tgaatgatga gtcgacgagt ggtggtgcag cgttgtcctg
     5581 ctgtacctac cgcaccgaat accaccgcaa gcaatgcaat gtcaatatcg gcattttcag
     5641 taatgataat agcattgttc ccgccaagtt caagcaatga tcttcccagg cgttctccaa
     5701 cggcttttcc tacagccttc cccattcggg tggatccggt agcagaaacc agcggcacgc
     5761 gcttatcgtt gctcatgagc tcacctaccg tatggtcacc gataattaca cacgaaacgc
     5821 cttccggtac ttcgttgcgt tttaacacat cggcaatgat attttggcag gcaatagcac
     5881 aaagcggtgt tttctcagag ggtttccaaa tacaaacgtc tccacaaacc catgccagca
     5941 tcgaattcca ggaccaaacc gcaacgggaa aattaaaagc agaaataatg cctacaattc
     6001 ccaatgggtg gtattgctca tacatgcggt gattcgggcg ctcgctgtgc atggttaagc
     6061 cgtgaagctg acgggaaagt ccaacggcaa aatcacagat atcaatcatc tcctgaactt
     6121 cgcccaatcc ttcctggtac gatttaccca tttcatagga tacaagcttg cccaattcgt
     6181 ttttgtattt gcgaagctct tcacccatct gacgaacaat ttcgccacgt ttaggcgcag
     6241 gtacttttct ccatacttta aaagcctcat tcgctttaag aatcaaggcc tcatattctg
     6301 cttctgtagc ggcatgtaca gaggcaattt tttctccgtt aacaggagaa taagaaacaa
     6361 tttcttcacc acgggtagca aaccagtaat taccggtaga tgcgcctgag ttcttgtctt
     6421 tgattcccaa aaccttcagg gtttcggcta taccgaagtc ggtcattact gctgacatat
     6481 cactaaaatt tagagtgcaa agttagccaa aaagactacc tgtaccaaag gaaaatatac
     6541 agcggtgata aatgctacac agaagcatta aaagagcttc agcacatcat tcaggttggc
     6601 gtacaagata agccccaata atagaagcat ccccactgtt tgagcaattt caaggaatcg
     6661 ctgattgggt gcttttccgc gaaccatctc ataaagtaaa aacaacacat gtccaccatc
     6721 gagcgcagga attggcaaga ggttcatgaa agcgaggatg atggaaataa atgcagtaag
     6781 gagccagaat tgttgccagt cccattcctt gggaaacatg cctccaatgg agccaaa
//
LOCUS       DAOMYG010000131         6826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3783_length_6826_cov_9.362576, whole genome shotgun sequence.
ACCESSION   DAOMYG010000131 DAOMYG010000000
VERSION     DAOMYG010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3783_length_6826_cov_9.362576"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PK610_05625"
     CDS             complement(<1..312)
                     /locus_tag="PK610_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35444.1"
                     /translation="MKNDEIVQIVYCEHFGWKEMLYIQLYIDGSFVLRSRKGADGMPV
                     SVRTTELSEEVKQLFLLSRNVFEENGIHFFKKNEDDCTRSLLFIGESQQRKKQLHYDE
                     KQ"
     gene            477..2846
                     /locus_tag="PK610_05630"
     CDS             477..2846
                     /locus_tag="PK610_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HRJ35445.1"
                     /translation="MKRLLFLAVFSMLSGLLSAQLNMTLRSHIDYQATRNSGCSNIWG
                     YVDENNNEYAIVGLNKGTSIVDVTDPNNPVEVFFIPDSNSIWREVKIWHDYAYVTTEE
                     AVGVMIIDLSPLPASTTLPVYRFYGNGGPSFTRAHSLFIDENGILYINGANRGNQGVI
                     FYDLNVSPTNPPEVGMYDVYYVHDSYARGDTLYAAHIYDGFFQVIDISTKSNPIVLAQ
                     QVTPMAFTHNCWISNDGDALFATDEVGGAPISAYDISDLGNIREIDQIKSNPGSNSIP
                     HNVLYRDGYLVTSYYRDGVVIHDAADPANLIEVGNYDVSPLTGNGFNSIWGAFPYFPS
                     GTIVASDIENGLYVFTPNYVRGCYLHGMVRNQVSNDPIANAQIAILNHPIVTSSLING
                     IYKTGIVSPGSYDIVFYKPGFEPDTVYNVNLIAGDTVFANGFLNPLNPITVTGQVLEQ
                     ITNAPIQGAIVEFRNQDFLFTTTTDAAGNYILSDMMAGTYDFSAGHWGHWNQCEENVS
                     FTASINSKNASLKKGYYDDFRLNFGWTSIDNAVSGNWVREIPIATGLPDGTPTNPHFD
                     VDTDCGKEAFITGNQLGTYTAADVDGGPAVLISPVFDATQYTDPWLAYYRWFFIRNTP
                     WAPNDTLFIYLSNGMDTVLLEAVHFFNTDLSQWIEVGFPISSFITPTANMRMILSIED
                     DQSLGYHILEAGLDRFYVSEGDQLSDGTVENKNGIRLFPNPNNGQFSVEMLNGSQIGN
                     WSIFDVNGRLIQAGNTEHSRLTLSDTGIEDGLYLFLTENGEALRLMIVR"
     gene            2892..4298
                     /locus_tag="PK610_05635"
     CDS             2892..4298
                     /locus_tag="PK610_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRJ35446.1"
                     /translation="MNENLLAGFTPLSTEKWIELLNKELKGKDYKDSLIWHTREGFSL
                     EPFYNTSSEAGSPGVYPYRRTRNTQSQQWEIDQEIQISAMPEEANKSALKALDSGASS
                     ITFCGTISSKNEMKSLLKDIGIQFIEVRFRGMNNPTEVYQWYAELFEQTGADSQAMRG
                     AVENDPISQGLLNGGFGEYHAHSVTKLAKERKANQGRFRTVTINASIYHNAGATTVQE
                     LAFALAHGHEYLNALVNAGLTIDDAASHIEFRFASSVDFYFSLAKNRAFRELWANVIK
                     AYQPKHSCSHNAFIIGETSGLFQSHQDPYSNLLRFTTEAFAGIAGGCDAFSIAAFDGT
                     NSDFSTRMGRNIQLLLREESHLDKIVDPAGGSFFVEELTQQLIQFAWEKFRSIEKDGG
                     ILNYIVTGKLVEECSFSAEQLRSDFRSKKIRMIGVNVYPDPQKKKADLTSKIPASLKE
                     FKALECFRISETEIPASV"
     gene            4298..4705
                     /locus_tag="PK610_05640"
     CDS             4298..4705
                     /locus_tag="PK610_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35447.1"
                     /translation="MIRNGFHIISTAQGGTGTFSKWSDGLLLYELEAGNDVHTTISDL
                     EKDVSAAKSVQNGERSIFLSDIRQLRHLSRDKQQFISKQMPGFSTAAAALVASPLSRI
                     VMNGFILLFRPDIPTRLFDDPEKAIEWLKKQAT"
     gene            4702..>6826
                     /gene="scpA"
                     /locus_tag="PK610_05645"
     CDS             4702..>6826
                     /gene="scpA"
                     /locus_tag="PK610_05645"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HRJ35448.1"
                     /translation="MRPEFPPITIVDSNENKNQPKAQENAWITPEQISLSSFYRKEDV
                     KDTVQSSFIAGAPPFLRGPYASMYAIRPWTIRQYAGFSTAEESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDHERVKGDVGKAGVAIDSVLDMKILFDQIPLNEMSVSMTMNGAV
                     LPIMAFYIVAAEEQGVKPEELSGTIQNDILKEFMVRNTYIYPPGPSMRIIGDIFRYTS
                     QHMPRFNSISISGYHMQEAGATADIELAYTLADGLEYLRTGRDAGLDIDDFAPRLSFF
                     WAIGMNPFMEIAKMRAARILWSKIVNQFNPKNPKSLALRTHCQTSGWSLTEQDPFNNV
                     ARTCIEALAAAFGGTQSLHTNALDEAIALPTDFSARIARNTQLYLQEETNICKQIDPW
                     GGSYYVEQLTNDLAQRAWELISEVEELGGMAKAIETGLPKLRIEEAAARRQARIDSGK
                     DVIVGVNAYLSDEKQEIDILEVDNQKVRLSQIERLKQLRSERDDLKVKKALADLEACA
                     KTGEGNLLELAVVAARLRASLGEISTALENVYGRYKAQIRAVSGIYSSEVKQDQDFIN
                     ACRLADEFASREGRRPRIMVAKLGQDGHDRGAKVIATSFADMGFDVDIGPLFQTPAEA
                     ARQAIENDVHILGVSSLAAGHKTLVPEVITELKKAGREDIIVIAGGVIPQQDYSYLFD
                     VGVATIFRPGTKIPKAAATPTSKRYE"
BASE COUNT         1977 a         1366 c         1518 g         1965 t
ORIGIN      
        1 ctgcttttcg tcgtaatgga gctgtttttt cctttgttgt gattcaccaa taaaaagaag
       61 actccgggtg caatcatctt cattcttttt gaaaaaatga attccgttct cttcaaaaac
      121 atttctgcta agcaaaaaaa gttgtttcac ctcttcagat aattcagtcg tacgaacgga
      181 aacgggcatt ccatcagcgc ctttacgcga acgcaaaacg aaagacccat ctatatacaa
      241 ctgaatgtaa agcatttcct tccagccgaa atgctcacaa taaacgatct ggacgatttc
      301 atcatttttc ataaggcaat attcgttgat gaaaatctta attcccaacg atttaatacc
      361 atattgcctg ttaagtgaac ggttcggtgg ccgttttttc gaattattct accctttcat
      421 cactgttttt ttggttgtgc tttcctttat gtactttgta gaatatttct ccaccaatga
      481 agcgactcct ttttcttgca gttttcagta tgctgtccgg attactttct gcacaattga
      541 acatgactct tagaagtcat atcgattatc aggcaacgcg aaattcgggt tgcagcaaca
      601 tttggggata tgtggatgag aacaacaatg aatacgccat tgttggattg aacaaaggaa
      661 cttcaatcgt agatgtaacc gatcccaata atccggtgga agtttttttt attcccgaca
      721 gcaattccat ttggagagaa gtaaaaatct ggcatgatta tgcctatgtc accaccgagg
      781 aagcagttgg agtaatgatc atcgatttga gtccattacc cgcaagtaca actttgccgg
      841 tttatcgttt ttatggcaat ggaggacctt cattcacacg ggcacactcc ttgtttatcg
      901 acgagaatgg aattttgtac atcaacggtg caaatcgagg aaatcaggga gtgatttttt
      961 acgaccttaa tgtaagtcca accaatcctc ccgaagttgg gatgtacgat gtatattatg
     1021 tgcatgattc gtacgcgaga ggagatacgc tatatgcagc gcatatttac gacggttttt
     1081 ttcaggttat agatatcagt acgaaatcca atcccatagt acttgctcaa caagttactc
     1141 caatggcatt tacccataat tgctggatca gtaacgatgg tgatgcactt tttgctactg
     1201 atgaagtagg aggagcgccc atttcagcat acgatatcag tgatctgggg aacatacggg
     1261 agatcgatca gattaaatcg aatccgggct ccaattcaat tcctcataat gttctttacc
     1321 gagacggtta tttagtcaca tcctattacc gagacggagt tgtaattcat gatgctgccg
     1381 atcccgcaaa tcttattgaa gtgggtaatt atgatgtttc tcctcttacc ggaaatggat
     1441 tcaacagtat ttggggtgcc tttccttatt ttccctcagg aacaattgtt gcttctgaca
     1501 ttgagaatgg attgtatgtt tttacaccca attatgttcg cggatgttat ctccatggta
     1561 tggttagaaa tcaggtgtcg aatgatccga tagctaatgc gcaaatcgct attttaaatc
     1621 acccgattgt tacttcttcg cttataaatg gaatttacaa aacggggata gtgagtccgg
     1681 gctcgtatga tatcgttttt tacaaacccg gatttgaacc tgatacggtt tacaatgtaa
     1741 atctgatcgc cggagacact gtttttgcaa acggttttct taatccgcta aatccgatta
     1801 cagttaccgg acaagtactg gaacaaatca ccaacgctcc tatacaaggt gcaatcgttg
     1861 aatttcgtaa tcaggatttt ttgtttacaa ccaccaccga tgccgctgga aattatattc
     1921 tcagcgatat gatggcaggt acttatgatt tttctgccgg tcattgggga cattggaatc
     1981 agtgtgagga gaatgtttca tttacagcat ccatcaattc gaaaaacgcg agtttgaaaa
     2041 aagggtatta cgacgatttt cgactcaatt tcggttggac aagcatcgat aatgcagtca
     2101 gtggaaactg ggtgagagaa atacctatag caaccggatt accagatggc acacctacca
     2161 atccgcattt tgatgtggat acagattgcg gcaaagaagc atttattacc ggaaatcagt
     2221 taggaaccta cactgctgct gatgttgatg gtggtccagc agtgctgatc tctccggtgt
     2281 ttgatgcaac gcagtatacc gatccctggc tggcttatta ccgctggttt tttatccgta
     2341 atacaccctg ggctcctaat gataccttgt tcatttacct aagtaatgga atggatactg
     2401 tgctattgga agctgtacac ttttttaata cagatctgtc gcaatggatt gaagttggat
     2461 ttccaatttc ttctttcatt acgccaactg ccaatatgcg catgatactt tccattgaag
     2521 atgatcaaag tcttggttac catattctcg aggccggact cgatcgtttt tatgtgtcgg
     2581 agggagatca gttaagtgat ggtactgtgg aaaataaaaa cggaattcgg ctttttccaa
     2641 atcccaataa cgggcaattc tcggttgaaa tgttgaacgg atctcaaata gggaactgga
     2701 gtatttttga tgtgaacgga cgattgatac aggcgggaaa tacggaacat tcgaggttaa
     2761 cgctctcgga tacagggatt gaagacggat tatacctctt tttgaccgaa aatggtgaag
     2821 ccttgcgact aatgattgtc cgctgacagg tgagggggaa ttgctaaatt agcagcctca
     2881 atctaccatc aatgaacgag aatttacttg caggatttac ccccttatca acggagaaat
     2941 ggattgagct tctgaacaag gagcttaaag gaaaagatta caaggattct ctgatatggc
     3001 atacgcgtga gggattttca ctggagccat tttacaacac gtcaagcgaa gccggttcac
     3061 ccggagtgta tccttaccgc cgtacacgca atacccaatc tcaacaatgg gaaattgatc
     3121 aggaaattca gatcagtgct atgccggagg aagcaaataa atcagcatta aaagcgcttg
     3181 attccggagc ttcatcaatt acattttgcg gtacgatttc ttcgaagaat gaaatgaaat
     3241 cattgttgaa ggatatcggt atccaattca ttgaagttcg ttttcggggt atgaacaatc
     3301 caactgaagt ttaccaatgg tatgcagaac tttttgagca aacaggagca gattcgcagg
     3361 ccatgcgagg tgcggtagaa aatgatccga tttcacaggg attattaaat ggaggttttg
     3421 gagaatacca cgcacattcc gttacaaaac ttgcaaaaga acggaaagcc aatcagggac
     3481 gattccgtac tgtaacgatt aacgcatcga tttatcataa tgcaggtgca acaacagttc
     3541 aggaattagc atttgcgctt gcacatggac atgaatattt gaatgcactt gttaatgcgg
     3601 gattgactat tgacgatgca gcttcgcata ttgaattccg atttgcatca tccgtagatt
     3661 tttattttag tcttgcaaaa aaccgtgcat ttcgtgagtt atgggcgaac gtaattaaag
     3721 cgtatcaacc gaaacattcg tgttcacata atgcctttat cattggagaa acttcaggtt
     3781 tattccaatc gcatcaggat ccctattcca atttgttgcg ttttaccacc gaagcatttg
     3841 caggaatagc aggtggatgc gatgccttta gtatagcagc attcgatggt acaaactccg
     3901 atttcagtac cagaatggga agaaatatcc agctcttgct ccgtgaagaa tcgcatttgg
     3961 ataagattgt tgatcctgcc ggaggatcat tttttgtgga ggaacttact caacaactca
     4021 ttcagtttgc atgggaaaaa tttagaagta ttgaaaaaga cggaggaatt ctgaattata
     4081 ttgtaacagg aaaactggtt gaagagtgtt cattttctgc tgaacagtta cgatccgatt
     4141 tcagatctaa aaagattcgg atgattggtg taaatgtata tcctgatccc caaaaaaaga
     4201 aagcagattt aacttctaaa attcccgctt ctctaaaaga attcaaagca ctggagtgtt
     4261 tccgtatttc cgaaactgaa attcctgctt ccgtttaatg atcagaaacg gattccatat
     4321 catttccacc gctcaaggtg gcaccggcac attttcgaag tggtcggatg gtttgttgct
     4381 ctatgaattg gaagccggaa atgatgtgca tactacaatt tctgatctgg aaaaagatgt
     4441 atcagcagcg aaatctgttc aaaacggaga acgatcgatt ttcctttccg atattcgtca
     4501 actccgtcat ttaagtcgcg acaaacaaca atttatttca aagcagatgc ctggattttc
     4561 tacggcagct gcagcattgg ttgcatcacc tttatcacgt attgtcatga acggattcat
     4621 tttattattc cgtcctgata ttccaacccg cttatttgat gatcctgaga aggcaattga
     4681 atggctaaaa aaacaggcaa catgagacct gaatttccac caatcacaat tgttgattcc
     4741 aacgaaaaca agaatcaacc caaagcacag gaaaatgcct ggattacacc ggaacagatt
     4801 tcactttcat cgttttaccg aaaggaagat gtaaaagata ctgttcaatc ttcttttatt
     4861 gcaggagcac caccattcct gcgcggaccc tatgcgagta tgtacgctat tcgtccctgg
     4921 accattcgtc agtatgccgg attttccact gcggaagaat cgaatgcatt ttaccgccgc
     4981 aacctcgctg ccggtcaaaa aggtttgtct gttgccttcg atcttgctac acatcgcgga
     5041 tacgacagtg atcatgagcg ggtaaaaggt gatgtaggaa aagcgggtgt cgctatcgat
     5101 tcggttttgg atatgaaaat tcttttcgat cagattccgc tgaatgagat gtcggtttcc
     5161 atgaccatga acggtgctgt attgcccatc atggcatttt acattgttgc agcagaagaa
     5221 caaggagtta aacctgaaga attatcagga acaattcaga acgatattct gaaagagttt
     5281 atggtccgca atacgtacat ctatcctccc ggaccgagca tgcggattat cggtgatatt
     5341 tttcgatata cttcacaaca catgccgagg tttaacagca ttagcatttc cggataccac
     5401 atgcaggaag caggtgcaac agcagatatt gaacttgcgt atactctggc cgacggactt
     5461 gaatatttac gcaccggtcg cgatgctggt ttggatattg atgattttgc accgcgactt
     5521 tccttctttt gggctattgg tatgaaccca tttatggaga ttgccaaaat gagagcagca
     5581 cggatattgt ggagcaagat tgtaaaccaa ttcaatccga aaaatccaaa atcacttgca
     5641 ttgcgaacgc attgtcagac atcaggatgg agtttaacgg aacaggatcc ctttaacaat
     5701 gttgctcgca catgcattga agcattggca gctgcatttg ggggaacaca gtccttgcac
     5761 accaacgctc ttgatgaagc cattgctttg ccaaccgatt tttctgcacg aatagcccgc
     5821 aatacccaat tgtacctgca ggaagaaaca aatatttgca aacaaattga tccctggggc
     5881 ggttcgtatt atgtggaaca gctgacaaac gatttggcac aacgagcatg ggaactgatc
     5941 tctgaggtgg aagaattggg tggtatggcc aaggcaattg aaacaggtct gccgaagtta
     6001 cgcatcgaag aagctgccgc acgcagacag gcacgtatcg attcgggtaa ggatgttatt
     6061 gtgggggtga atgcttatct ttcggatgag aaacaggaaa ttgacatcct ggaggtggat
     6121 aatcagaaag tacgtttgtc gcaaattgag cgattgaaac aattgcgaag cgaacgtgat
     6181 gatttgaaag tgaaaaaagc attggcagat ctcgaagctt gtgcaaaaac gggtgaagga
     6241 aatttattgg aactcgctgt tgtggcagca cggttgagag caagtctggg agaaatttct
     6301 actgcgctcg aaaatgttta cggaagatac aaagctcaaa tcagggcagt atccggaata
     6361 tattcttcag aagtgaaaca ggatcaggat ttcattaatg catgtcgcct cgctgatgaa
     6421 tttgcatcgc gtgaaggtcg tcgtccgcgt atcatggttg ctaaacttgg tcaggatggt
     6481 cacgaccgtg gggcaaaggt tatcgcaact tcgttcgccg atatgggctt tgatgtggat
     6541 attggtccgc tgttccaaac ccctgctgaa gctgctcgtc aggctataga aaatgatgtt
     6601 catattcttg gggtttcttc attagcggca ggacataaaa cgcttgttcc ggaagtaata
     6661 actgagctaa aaaaagcagg acgagaagat atcattgtca ttgcaggagg cgttattccc
     6721 cagcaggatt attcgtatct ctttgatgtg ggtgttgcta cgatattcag accgggaacg
     6781 aaaattccaa aagctgctgc aacacccaca tcaaagagat acgaat
//
LOCUS       DAOMYG010000132         6807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3803_length_6807_cov_10.001185, whole genome shotgun sequence.
ACCESSION   DAOMYG010000132 DAOMYG010000000
VERSION     DAOMYG010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3803_length_6807_cov_10.001185"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PK610_05650"
     CDS             complement(<1..263)
                     /locus_tag="PK610_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRJ35449.1"
                     /translation="MNISDRDQATIWHPFTQMKLQGKLPAVVGAEGTKIFLEDGSVLL
                     DMISSWWVNVHGHAHPHSIERITEQVKKLDQVIFAGFTHEPAVV"
     gene            complement(324..968)
                     /locus_tag="PK610_05655"
     CDS             complement(324..968)
                     /locus_tag="PK610_05655"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HRJ35450.1"
                     /translation="MRTIFFLTLFLAGGFSLFAQDKDPKAKAVLDEVSKKIKGYTSYS
                     VEFSTNLKKDKVDENYNGKAIIKGDKYIIETKDQKIISDGKTVWTILLKEKEVYENPI
                     DESGDDELLNPTKMFNLWEKDFKYKWIKEEAVGGVTLIEIHLFPTNPAKAKFHTVIVK
                     INKATNEVHSVIVKGKEGDVLTYKLLKLVPNVPINDADFKFDKAKYPGYTIYKD"
     gene            complement(965..3532)
                     /locus_tag="PK610_05660"
     CDS             complement(965..3532)
                     /locus_tag="PK610_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRJ35451.1"
                     /translation="MAAKKNKFIEQSSEQDSSVADRPDRKERKKEKKPAAERPVSNRF
                     AFLRDERFQKIFGGALLLGALYLLIAFTSFLFTWKADQDKVESMSWLAFVFSSSDVVV
                     ENWLGKMGALLSHLFIHSGFGIASYLFILNFFIFGFRLITGIQLLPAGKMIKLSLLGL
                     ILVSATLGFLFHTGEWIFLGGAFGFHLNGWLIASLGKIGTLLLLGFSYFIIVVVYFNP
                     SFLIKRKEALQDGDVPPLDEEGKINIYNPTKEEDIIAEKKSQTIDLSRPQEEIPVSRV
                     DDEIRNEEVITEILLAEHEAEKAAATEAEELSSTVTTADDETASDELLIEIPDHGEGE
                     LNEDEVKNRLMEFGEYDPTLELSSYKLPSLDMLESYGGGTIVINKEELEQNKNRILDT
                     LRNYNIEIAKIKATVGPTVTLYEIIPAPGVRISKIKNLEDDIALSLSALGIRIIAPIP
                     GKGTIGIEVPNSKAEIVSMRSVVASEKFQHSDMELPVVLGKTISNETFVTDLAKMPHL
                     LMAGATGQGKSVGLNAILVSILYKKHPSQVKFVLVDPKKVELTLFNKIERHFLAKLPD
                     TDEAIITENKKVVHTINSLCIEMDARYELLKDAGVRNIKEYNAKFIQRRLNPEHGHKF
                     LPYIVLVVDEFADLIMTAGKEIETPIARLAQLARAIGIHLIIATQRPSVNIITGTIKA
                     NFPARIAFRVTSKIDSRTILDSGGAEQLIGRGDMLFSTGNDLIRIQCAFVDTPEVDQI
                     CEYIGGQRAYPSAFLLPEYVGEESDELGGAGDDGDRDDLFEDAARIVVSTQQGSASLL
                     QRKMKLGYNRAGRIIDQLEAVGIIGPFKGSKAREVLIKDVQVLEQFLNSLKNKDTNL"
     gene            complement(3626..4291)
                     /locus_tag="PK610_05665"
     CDS             complement(3626..4291)
                     /locus_tag="PK610_05665"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HRJ35452.1"
                     /translation="MKRILFFATVAVALLTAFAPGKDKKKSPGYEGKISYSMTYEDLP
                     AELENYASMLPKEMTMYIKGEQSRTEQASGMGTTITIVDKKKKEICILMDMMGAKTAY
                     KGSTDEMKDTTVKAKPEIKVTEETREIAGKKCSKVEITYPGEDAEVVWVTKDMPSGLT
                     RGFESLDGFPMEYTIKRSGMTILLTVTAVAEQKVSAEYFKVPDGYTVKPMSEMKNMMG
                     GGK"
     gene            complement(4306..5013)
                     /locus_tag="PK610_05670"
     CDS             complement(4306..5013)
                     /locus_tag="PK610_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35453.1"
                     /translation="MVNASLIYRLRSAIVACLAVVMLSSCGSDKVYEGTIEFDVSYPY
                     FRASGFMAGMLPDKMVMRFKGNKYKTEVKKGRAFSSSFIIDCDNKTLTTMFQFGQRRK
                     YAILEEAAVLKRRDEKFPIPTYMHTNEGDSIVGFLCRKSVAIFEELGQPEATLYYTDE
                     IGIKNPNWCFPYHAIDGVMLIYELQQFGIRMRFTAEKFDPAPIDDKIFEVPSNYNAVP
                     FAELEKEMEEMFSTIVD"
     gene            complement(5073..6464)
                     /locus_tag="PK610_05675"
     CDS             complement(5073..6464)
                     /locus_tag="PK610_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HRJ35454.1"
                     /translation="MENQYLDLIKQTFDFPTESFTVNDNELYFNDIPLMDIVKQYGTP
                     LKITYLPKIGDQIQKAKTMFNVAMAKVDYQGKYTYCYCTKSSHFSFVLEEVLKNGGHI
                     ETSSAYDMYIVKDLYERGFLTKEHYIICNGYKRDLYTEQVIELINEGFENLIPVLDNK
                     EELASYAEGIKKNVKIGIRIASEEEPTFDFYTSRLGIRYIDIIDFYTTHIKKNPNFEL
                     KMLHFFINTGIKDNIYYWSELAKCLNIYYRLKQVCPTLDTLNIGGGLPIRNSLNFEYD
                     YEYMIEQIVQQIKKFCVQKGVPEPNLFTEFGSYTVGESGANIYSVMSAKQQNDREMWY
                     MVDNSFITTLPDTWGINQRFILLAINNWDHEYLRVNLGGLSCDSQDYYNSEAHDNQVF
                     LPKFDKEEPLYIGFFHTGAYQESLSGYGGIKHCLIPSPKHVLIDRDENGEITTKLFAK
                     EQSAKSMLKILGY"
     gene            6558..>6807
                     /locus_tag="PK610_05680"
     CDS             6558..>6807
                     /locus_tag="PK610_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35455.1"
                     /translation="MNTLSIIEIKSEIGAGTRGASLGPDAIKVAALNKGDDLFFKNKP
                     VHVLAENYLLHTPVTTPNGKRISGLVRIFERITKAVSTE"
BASE COUNT         1882 a         1572 c         1329 g         2024 t
ORIGIN      
        1 acaactgcag gttcatgcgt aaatccggcg aagataacct gatccaattt tttcacctgc
       61 tccgttattc gctcaatgct gtgtggatga gcatgaccat gtacattcac ccaccacgaa
      121 gaaatcatat cgagtaacac actgccatct tccagaaaaa tcttcgtccc ttcagcccca
      181 acaacagcag gaagttttcc ctgcaatttc atttgggtaa agggatgcca gatagtggcc
      241 tggtcgcgat cgctgatatt cattccgcca aattagcaca aaaaaaaaga gaccctgagg
      301 tctctttctg ttgaagcaaa gaattaatcc ttgtaaatcg tataaccggg gtatttggct
      361 ttatcaaatt tgaaatcggc atcgtttata ggcacattag gtaccagttt caataatttg
      421 tacgtcagca catcgccttc ttttcctttt acaatcacac tgtgtacttc gttggttgcc
      481 ttattgatct tcactatcac cgtgtggaat ttcgctttgg caggattagt cggaaagaga
      541 tggatctcaa taagtgttac acctccaacg gcttcttcct tgatccattt gtatttgaaa
      601 tccttctccc aaagattaaa catcttggtt ggattcagca actcatcatc accgctttca
      661 tcgatcggat tttcatagac ttccttttcc ttcagaagaa tggtccaaac cgttttacca
      721 tcactgatga tcttttgatc cttggtctca atgatgtatt tatcgccctt gataattgct
      781 ttcccgttat agttttcatc cactttatct tttttgaggt tggtggaaaa ctcaacacta
      841 tagctggtat agcctttgat ttttttgctg acttcatcca atacggcctt ggccttgggg
      901 tctttatcct gtgcaaaaag gctgaatccg ccggcaagga aaagggtcag aaagaagatg
      961 gttctcataa gtttgtgtct ttgttcttta ggctattcaa aaactgttcc aaaacctgca
     1021 catccttaat caggacttca cgggccttgg atcctttgaa gggtccgatg atgccaactg
     1081 cctcaagctg atcgattata cgtcctgccc tgttgtatcc cagcttcatt tttcgctgaa
     1141 gcaaagaggc cgaaccttgt tgggtgctga caacaatgcg tgctgcatcc tcaaataaat
     1201 catcccgatc gccatcatct cctgctccac ccaattcatc gctttcttca ccaacatatt
     1261 caggcagcaa aaaggcgctc gggtatgcac gctgaccacc aatgtactcg cagatctgat
     1321 ctacttctgg agtatccaca aacgcacact gaatacggat gagatcgttt cctgtgctga
     1381 acaacatatc accacgccca atcagctgtt ctgcacctcc agaatcaaga attgtgcggg
     1441 agtcaatttt ggaagtaacc ctaaacgcaa tacgcgccgg aaaattggct ttaatggttc
     1501 cggtaatgat atttacagaa ggacgctgtg tggcaataat gaggtgaata ccgatagcac
     1561 gggcaagttg tgcaagtcgg gcaatgggtg tttcaatttc ttttcctgcc gtcatgatca
     1621 aatctgcaaa ctcatccacc accaaaacaa tgtaaggaag gaatttatgt ccgtgctcag
     1681 gatttagtcg gcgctgaatg aattttgcat tgtactcttt gatgtttcgc acaccggcat
     1741 ccttcagcaa ttcataacgc gcatccattt cgatacataa tgaattgatg gtgtgcacca
     1801 cctttttgtt ctcggtaatg atcgcttcgt cggtatcggg tagtttggcg aggaaatgcc
     1861 gctcaatctt gttgaataaa gtcagctcca cctttttcgg atcgacaagg acaaatttta
     1921 cctgtgaagg atgtttcttg tagagaatag aaaccagaat ggcatttagt cctacagatt
     1981 taccctgacc ggtagcacct gccatgagaa ggtgaggcat ttttgcgagg tcggtaacaa
     2041 acgtttcgtt cgaaattgtt tttccaagca caaccggaag ttccatatcc gaatgctgga
     2101 atttttccga agcaaccaca ctgcgcatcg aaacgatttc ggctttggaa ttcggtactt
     2161 caataccaat ggttccttta ccgggtatcg gtgcaatgat acgtatgcca agtgcagata
     2221 aacttagtgc gatatcatct tccagatttt tgatcttcga aatacgcact cccggagcag
     2281 gaatgatctc gtacaaggta actgtaggac caacagtggc ttttattttg gcaatctcaa
     2341 tattgtaatt gcgcagcgta tcaagaattc tgtttttgtt ttgctcgagt tcttccttgt
     2401 tgataacgat cgttcctcct ccataacttt ccagcatatc gagcgatggc agtttatacg
     2461 aggacaactc cagtgtcgga tcgtattcac caaactccat cagacgattt ttcacttcat
     2521 cctcattcaa ttcgccttca ccatgatccg gaatttcaat cagcaattcg tcagaagcag
     2581 tttcatcatc cgcagttgta actgtgctac tcaattcttc cgcttcagtt gcagctgctt
     2641 tttcagcttc gtgttcggcc agcagaattt cggtaatgac ttcctcgttc ctgatttcat
     2701 catcaacgcg cgacaccgga atctcttcct gcggacgact cagatcgatg gtctgacttt
     2761 tcttttcagc aattatatct tcctctttgg tgggattgta tatattaatc ttcccttctt
     2821 catccaatgg aggcacatca ccatcctgca acgcttcctt gcgtttgatc agaaaggaag
     2881 gattaaaata aacgactaca ataatgaaat aggaaaatcc gagtaagaga agcgttccga
     2941 tttttccaag tgatgcaatc aaccagccat tcagatgaaa gccaaaagct ccgccaagaa
     3001 aaatccattc gccggtatga aacaaaaaac cgagtgttgc agaaaccaaa atcaatccca
     3061 acaaggacag cttgatcatt tttcctgcag gtaagagttg tattccggta atgagacgaa
     3121 aaccgaaaat gaaaaaatta aggataaaca aatacgatgc aataccgaat ccggaatgga
     3181 taaaaaggtg agaaagcaag gctcccattt ttcccagcca gttttcaacc acaacatcag
     3241 aagaagaaaa cacgaaggcg agccacgaca tcgattcaac tttatcctga tccgctttcc
     3301 aggtaaacaa gaacgaggta aacgcaatca gcaaataaag cgctcccaat aacaatgcac
     3361 ctccaaaaat tttctgaaaa cgttcatcac gcaaaaaagc aaaacgattg gaaaccggac
     3421 gttctgccgc aggtttcttt tcctttttgc gttccttacg atcaggtctg tcagcaacag
     3481 aagaatcctg ttctgaagac tgctcaataa acttattttt tttggctgcc atccgtaaat
     3541 tagggtaagc taaagtagcg cctatttccc ctaatgaagg ctggtcttaa agtgtagtat
     3601 gaacagtgaa gtgttgatgg ccggattatt ttccgccacc catcatgttc ttcatttcac
     3661 tcatcggctt tacggtatag ccgtcgggca ctttaaaata ttctgcagat actttttgct
     3721 cagccacggc agttaccgtt aagagaattg tcataccact tctcttgatg gtatactcca
     3781 ttggaaaacc atccaaagac tcaaaaccac gcgtcaaacc ggaaggcata tcttttgtga
     3841 cccaaactac ctctgcatct tcaccgggat aggtgatttc aaccttactg cactttttac
     3901 ccgcaatttc acgagtctct tcggtgactt taatttccgg cttcgctttt acagtggtgt
     3961 ctttcatctc gtcggtagag cccttgtacg ctgttttcgc ccccatcata tccatcagga
     4021 tacagatctc cttcttcttc ttatcgacaa tcgtaatggt tgtccccatt ccgctggcct
     4081 gttctgtgcg cgattgttcc cctttgatgt acatggtcat ttccttgggc aacatcgaag
     4141 cataattttc cagttccgcg ggtaaatcct cgtaggtcat ggaatagctg atcttccctt
     4201 cataaccggg agattttttc ttgtctttac cgggggcaaa tgcagtaagc aaagcaacag
     4261 ctaccgttgc aaaaaagaga atccgtttca tggtaaattg agttttcagt cgacaatcgt
     4321 gctgaacatt tcttccattt ccttttcgag ttcggcaaaa ggaacagcgt tgtaattgga
     4381 aggaacttca aagatcttat catcaatagg agccggatca aacttctcag ctgtaaatcg
     4441 catccggatc ccgaattgct gcaattcgta aataagcatt acaccatcaa ttgcgtgata
     4501 agggaaacac cagtttggat tttttattcc gatttcatct gtgtaataaa gcgttgcttc
     4561 aggctgacct aattcttcga aaatggcaac actttttctg cataaaaatc caacaattga
     4621 atctccttca ttggtatgca tgtaagtggg aataggaaac ttttcatctc tgcgtttcag
     4681 tactgcagcc tcctcaagaa ttgcatactt tctgcgctga ccaaactgaa acatggtcgt
     4741 aagtgtttta ttgtcgcaat caataatgaa tgaacttgaa aacgcgcgtc cctttttaac
     4801 ttcggttttg tacttgtttc ctttgaaacg catcaccatt ttatcgggta acattcccgc
     4861 cataaatcca cttgcgcgga aatacggata gcttacgtca aactcgatgg taccctcata
     4921 tactttatca cttccgcagg atgataacat caccactgcc aggcaggcaa ctattgcact
     4981 gcgtaatcga taaataagtg aggcattcac catgaaggca caatttcagc ccacaaatat
     5041 atcagaaatt cggacaaacg gtgtattgac cattaatagc ctaaaatctt caacatcgac
     5101 ttggcactct gctctttagc aaaaagcttg gtcgtaatct ccccgttttc atcacggtcg
     5161 atcagtacgt gcttgggact tggaatcaaa caatgcttga ttccgccgta gcccgaaagc
     5221 gattcctgat aggctcctgt gtggaaaaac ccgatgtaca atggttcttc tttatcaaat
     5281 ttcggaagaa acacctggtt atcatgcgct tcagaattgt aataatcctg actgtcacaa
     5341 cttaatccac ccaggttaac ccgcaggtat tcatggtccc agttatttat tgccaaaaga
     5401 ataaagcgct ggttgattcc ccatgtatcc ggaagagtgg tgataaagga attatcgacc
     5461 atgtaccaca tctcgcggtc gttttgctgc ttggcgctca ttaccgaata gatatttgct
     5521 ccgctttcgc caacagtgta ggatccaaac tcagtaaaaa gattgggctc gggaactcct
     5581 ttttgcacac agaatttttt gatctgctgt acgatttgct cgatcatgta ttcataatcg
     5641 tattcgaaat tcaatgaatt acgaataggc aatccaccgc cgatgttcag tgtatccagg
     5701 gttggacaaa cttgtttcaa acgatagtag atattcaggc attttgccag ctccgaccaa
     5761 taatagatat tatccttaat tccggtattg atgaagaaat gcaacatctt caattcgaaa
     5821 ttgggattct tcttaatgtg agtagtgtaa aaatcgatga tgtcgatata ccgaattcca
     5881 agacgggaag tataaaaatc gaaagtcggc tcttcttcag atgcaatccg gattccgatt
     5941 ttcacatttt tcttgatccc ttccgcatag cttgccaact cctctttgtt atccaataca
     6001 ggaatcagat tttcaaatcc ttcattgatc aattctatga cttgttccgt atacaaatcg
     6061 cgtttgtacc cattgcaaat gatgtaatgt tctttggtaa gaaaaccgcg ttcgtacaag
     6121 tcttttacga tgtacatatc gtatgcggaa gaggtctcga tatgaccgcc attcttcaac
     6181 acttcttcca atacaaagga gaaatgcgaa cttttagtgc agtaacaata tgtatatttg
     6241 ccctgataat cgaccttggc catggccacg ttgaacattg tctttgcctt ctgaatctga
     6301 tcaccgattt tcggcaggta agtaattttt aacggagtac catactgttt tacgatatcc
     6361 ataagcggta tatcattaaa atacaactcg ttatcgttta ctgtaaagct ttctgtcggg
     6421 aaatcgaagg tttgcttaat caggtcaagg tactggtttt ccattagttg cgatttaact
     6481 cgattagatc gttaattttc aagcaaagta agtcacccgg attgaaaccg ggccgcgaat
     6541 gtaaataaat tagtgttatg aatacgttaa gtatcataga aatcaaatca gaaattggag
     6601 ccggaacccg gggagccagc cttggtccgg atgcgattaa ggtagctgca ctcaataaag
     6661 gagacgatct cttctttaaa aacaagcctg tgcatgttct tgctgaaaac tatctgcttc
     6721 atacaccggt aactacccct aacgggaaac gtatttccgg tttggtgcgg atctttgagc
     6781 gcatcaccaa agccgtttcc actgaat
//
LOCUS       DAOMYG010000133         6802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3807_length_6802_cov_8.715281, whole genome shotgun sequence.
ACCESSION   DAOMYG010000133 DAOMYG010000000
VERSION     DAOMYG010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3807_length_6802_cov_8.715281"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="PK610_05685"
     CDS             complement(<1..1002)
                     /locus_tag="PK610_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35456.1"
                     /translation="MILQRPLFWFFLLSFGFVGTHLYLTYYGIPFAAFLPFGLLVAFT
                     ALFRLDWVYWMVVAFTPLSININEFGILDLGLYLPTEPLLFGVLVLFVGKMIVEGGYD
                     RRYLRHPVSILILLQLFWMFVTAITSQDVLVSLKYVLARTWFVVPGYFFVMLLLHEKK
                     TIRLMIWLSILPLCAVVLYTLVNHAMHGFEEKPAHWVMSPFYKDHTSYGAILAMYIPV
                     AIGMYFNKSLSPIVRALIVFISTVLVLGVIFSYTRAAWVSLIGAFGLLMVLLLRIRLS
                     TIVLGLALGGFVFFSFYDQIIMNLERNKTDSSDNLADHATSISNISSDASNLERINRW
                     "
     gene            complement(1005..2012)
                     /locus_tag="PK610_05690"
     CDS             complement(1005..2012)
                     /locus_tag="PK610_05690"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HRJ35457.1"
                     /translation="MTNPKPAGDFSSTTLLLLLWKWRMRIIIVMGVTVVVSSVVALMI
                     REKYKSTAIIFPAKSSSIVLGKMLNPSQGILQFGEEEEAEQVLQILNSSQIRDQIISK
                     YNLMQHYEIDTASKFKNTELVKEYEDNVKCSRTRYGSVTIEVLDYHPDTAALIANDIA
                     RLLDSAKNKMLQDRGGEAFRVVEQEFLALKSDVDALNDTLTKLGEMGVIGSSAGVAAL
                     YEAQANAIQARNELALRKIDEQINLNKKYGAAYSSFLEQRELKNMRLDEMEGTYLQAR
                     ADAYSNVSHHFDVEQATPAEKKSYPVRWLIVFISVITSFFFMIFFIIALEKIRELRQM
                     A"
     gene            2100..3233
                     /locus_tag="PK610_05695"
     CDS             2100..3233
                     /locus_tag="PK610_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRJ35458.1"
                     /translation="MRIAVNTRFLLPGKMEGFGWYSYETLRRIVVAHPEHEFIFLFDR
                     PFDQRFVFASNVTPVVIGPQARHPFLFWIWFEVSVKRALIKYKADVFVSPDGYLSLGT
                     NVPSVAVMHDLNFEHYPKDLGWLVRNYYKRYFPKFARKADRIVTVSESSRKDIIDTYG
                     ISPEKIDVSYNGVNEDLHPLTPDECEKVREQYTEGKPYFIFVGALHPRKNLARLFHAY
                     DLFCDRNKNSHKLVIVGEKYLWNSEINTAFSRMKHRESVVFTGHLPMEQLGNVLAAAR
                     ALVFVSVFEGFGLPIVEAMRCEVPVITSSVSCMPEIAGGAALLCDPFSVEAIAEALDR
                     MANDSGLREQLIAKGKENGKRFSWNHAAEGLWNSIEKTLHAKK"
     gene            3220..3819
                     /locus_tag="PK610_05700"
     CDS             3220..3819
                     /locus_tag="PK610_05700"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRJ35459.1"
                     /translation="MLKSRFSKEFTEAGCDEAGRGCLAGPVVAAAVILPEGYKHRFLN
                     DSKKLTEKVRLELRWEIMETAVAWAIGSCSPKEIDEINILKASFEAMHRAITQLKQKP
                     QLLLIDGNRFTPYAGIDHQCIIGGDAKYLSIAAASILAKTERDMIMEKLHEEHPLYHW
                     NQNKGYPTAQHRKAIEQFGSTFHHRKSFTLLPPQMKLEF"
     gene            3904..6636
                     /locus_tag="PK610_05705"
     CDS             3904..6636
                     /locus_tag="PK610_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35460.1"
                     /translation="MLRKILNIFAITLLLVFTIWLGIRLFHRIANPQADLSHAVPNDA
                     ALVFRLSDPILLWNQLNSSSLLWSELKSVHPFRQTHNALAFCDSLITTDTEFRSSFSG
                     KPVLFSVHPAGNKGIETMLAIELPSGGEKKCLSLLERVLKQTFSVREFKSESIYNLTL
                     GNAPWQILIKENFLTISSSATLAERISMQLDQDISIATEAGWKEVYATSDIGAQANIF
                     IHHQNFSKLIHALVQEDQNDALTGIYPYAYFTEFDLSLRSGTVSLNGFSFAPDSLGSM
                     LNIFSKHEPQPFEVARYLPDNTAAFLWLGINDGESFRTSIDAYRQSKGTLQQYRKEIN
                     SFNADKDCNIERQLVSWIGNEIVLFSNGKADTSAKKENLFLAIRINDMADPISELNAL
                     ADKLDTNATRIRDQDGMQMRRLLANDLFSFLFGPAFDGIDNPWYIRIEDYMIFANSGE
                     ALKQYLMDISSDRTLGKNITYYNFVSENLSDRANLSVYVNPSKFKSLLPQFFNNTTSE
                     ELENPNSLLWKFDALAWQFSNSGKGMFYNNLYLKYNGTGKQDSKSLWEVAVDTTVNMQ
                     PLLVKNHITGTIDVVVQDENYSFYLISSKGEILWKKQLEERLTGDVRQIDFFGNGKLQ
                     LLFATTTAIHLIDLKGNYLKGFPYRLKEEVSGPLGVFDYESNNNYRILIPCGRNIVNI
                     DKEAKVTTGWEPEKFSSSINQAPVFFRCDNKDYLFVIDTKGEIRIIDRKGKPRYNLSF
                     GLENRSSNKVLLEQGSSIEGTSLIYSDTSGRIIRTFLNGNADTARTGLKSSSHRFAYI
                     DANEDGKKDIVLLDSNKIDITEWSGKRIGVYHFPERVSSHLMVFALKDGSIRIGAVAP
                     GVGEIYLLNSSGQLHSKFPLTGTTAFPLSDINGDGSLEVVSGTGSRRIVCYSLN"
BASE COUNT         2082 a         1500 c         1375 g         1845 t
ORIGIN      
        1 ccaacgattg attcgttcca gattcgaagc atccgaggaa atattagaaa ttgaagttgc
       61 gtgatcagca agattatccg aagaatccgt tttatttctt tccaggttca taatgatctg
      121 atcgtagaac gagaaaaaaa caaagcctcc cagtgccaat ccaagtacaa tggtagaaag
      181 tcgaatacgc aacaataaaa ccatgagtaa tccgaatgca ccaataagac tcacccacgc
      241 ggcgcgggta tagctgaaaa taacacctaa cacaagaaca gtgctgatga aaacaatcaa
      301 ggcccgaaca atgggactta aactcttgtt gaaatacatt ccgattgcaa ccggaatata
      361 cattgcgaga atggcgccat aggaagtatg atctttgtaa aaaggcgaca tcacccaatg
      421 cgcaggtttt tcctcaaagc cgtgcatggc gtggttaacg agtgtatata aaaccacagc
      481 acacaaaggt aaaatggaaa gccagatcat taagcggatc gttttctttt cgtggagtaa
      541 tagcattacg aaaaaatatc ctggcacaac aaaccaggtc cgggcaagta catatttaag
      601 tgaaaccaga acatcctgac tggtaattgc tgttacaaac atccagaaca attgcaggag
      661 aatgagaata gaaacaggat gacgaagata acgacgatcg tatccacctt caacaatcat
      721 ttttccgaca aacaacacca acactccaaa caataaaggt tcggttggca aatacaaacc
      781 gagatccaat attccaaatt cattgatatt gatggatagc ggagtaaatg caacaaccat
      841 ccaatacacc caatcgagac gaaaaagagc agtgaatgca accaataaac caaagggaag
      901 aaaggctgca aagggaattc cgtaataggt taaatagagg tgtgttccga caaaaccgaa
      961 agacaacagg aaaaaccaaa ataacggtcg ctgcaggatc atgatcaggc catctggcga
     1021 agttcacgaa tcttctcgag ggcaatgatg aagaagatca taaagaaaaa cgaagtgatt
     1081 accgagatga aaacgatcag ccaacgcacc ggatacgatt ttttctctgc cggagtagcc
     1141 tgttcaacat cgaagtgatg cgacacattg gaataagcat ccgcccttgc ctgcagatag
     1201 gttccttcca tttcatccaa tcgcatattt tttaattccc tttgctcaag aaaagaagaa
     1261 taagctgcac catatttttt gttcaggttg atctgctcat caattttgcg caaagcaagt
     1321 tcattgcgtg cctgaattgc gttggcctgc gcctcgtaca atgctgctac tcctgccgaa
     1381 ctaccaatta cacccatttc ccccaacttg gtcagcgtat cgtttaaggc atccacatcc
     1441 gattttaaag caagaaattc ctgctcaaca acacgaaatg cttctccacc acgatcctgc
     1501 aacattttgt ttttggcaga atccaacaaa cgggcaatat catttgcgat taaggcagca
     1561 gtatcagggt gataatccaa tacttcaatg gttactgacc cataacgcgt tcttgaacat
     1621 tttacgttgt cttcgtattc ttttaccaat tccgtgtttt tgaatttact ggcggtatcg
     1681 atttcatagt gctgcatcag gttgtactta ctgatgatct gatcgcggat ctgtgaagaa
     1741 ttgaggatct gtaggacctg ttctgcttcc tcctcttcac caaactgaag gataccctga
     1801 gaagggttca acatttttcc aagtacaata ctgcttgatt ttgctgggaa aataattgca
     1861 gtcgatttgt atttctccct gatcatcagc gccacaactg acgaaacaac cacggttaca
     1921 cccattacaa taatgatgcg cattctccat ttccagagca aaagcaataa ggtggttgaa
     1981 ctgaaatcac cggcaggttt tgggtttgtc atggttcgtt ttttaatatc aggcgacgaa
     2041 aatacggaaa aaggcgcgtt ttattgtctg cccccgaaaa tggctatctt gcatcgtaaa
     2101 tgcgcattgc tgttaacacc cgctttctac tgcccggaaa aatggaggga tttggctggt
     2161 atagctacga aacactacgc agaattgtgg tagcccaccc tgaacacgaa ttcatctttc
     2221 ttttcgaccg ccctttcgac cagcgcttcg tatttgcatc gaatgtaact ccggtggtga
     2281 ttggtccaca ggcgcgtcat ccctttttat tttggatatg gtttgaagta tctgttaaac
     2341 gagcactcat aaaatacaag gccgacgttt tcgtttctcc tgacggatac ctgagtcttg
     2401 gcacaaacgt gccctctgtt gcagtaatgc acgacctcaa ttttgagcac tatccaaaag
     2461 atctcgggtg gctggtgcgt aattattaca agcgctattt cccgaaattt gcccgcaaag
     2521 ccgatcggat tgtaacggta agtgaatcat cgcgaaaaga tatcatcgac acttatggaa
     2581 tttctccaga aaaaattgat gtttcctata acggagtgaa tgaagatttg catccgctta
     2641 cgcctgatga atgtgaaaaa gtccgcgaac aatacactga aggaaaacct tattttattt
     2701 ttgtaggggc attgcatccg agaaaaaatc ttgcgcgctt atttcatgct tacgatctct
     2761 tctgcgatag aaacaaaaac tctcataaac tggtgatcgt cggcgaaaaa tacctttgga
     2821 attccgagat caataccgcg ttttcacgaa tgaaacatcg cgaaagtgtg gtattcaccg
     2881 gtcatttacc aatggagcaa ttgggaaatg tactcgccgc tgcccgtgca ttggttttcg
     2941 ttagtgtttt tgaaggattt ggattaccca ttgttgaagc gatgcgttgt gaagtacctg
     3001 taataacatc aagcgtaagt tgtatgccgg aaattgccgg aggtgcagca cttctatgcg
     3061 atcccttttc tgttgaagct attgcagaag cattggatcg aatggcaaat gattccggtt
     3121 tgcgggagca attgatagcg aagggaaaag aaaatggtaa gcgatttagc tggaatcacg
     3181 cagcagaagg attatggaac agtattgaaa aaacgctgca tgctaaaaag tagattcagt
     3241 aaagaattca cagaagcagg ttgcgacgaa gcaggcagag gatgtttggc aggcccggtt
     3301 gttgcggctg cagtaatatt accggaagga tacaaacatc gtttcttaaa tgattccaaa
     3361 aaattaaccg agaaagtcag gcttgaactc cgatgggaaa tcatggaaac agcagtagcc
     3421 tgggccattg gaagttgttc acccaaggag atcgatgaga tcaacatcct taaggcctcc
     3481 ttcgaagcaa tgcaccgggc tataacgcaa ctcaaacaaa aaccacaatt acttcttatc
     3541 gatggtaacc gctttacccc ttatgcaggt attgatcatc agtgtatcat tggcggagat
     3601 gcgaaatacc tgagtattgc tgcagcatct attctggcaa aaacagaaag ggatatgatc
     3661 atggaaaaac tccacgaaga acatcctctc taccactgga accagaacaa aggataccct
     3721 accgcacagc atcgaaaagc tattgagcaa tttgggagta cctttcatca ccgaaagagc
     3781 tttaccttgc tacctcctca aatgaagctg gaattttgac ctgctaacat tactttgttt
     3841 atactctccc ctgttctctc ccctacaagc gtgacggaat cctaattttg atcccagatc
     3901 accatgctca gaaaaattct caatattttc gccatcaccc tgctccttgt gtttaccatt
     3961 tggctgggca tccgtttatt tcatcgcatc gcaaatccgc aagcagatct ttcacatgca
     4021 gttcccaatg atgccgcgct cgtcttccgc ctcagcgatc cgattttgtt gtggaatcag
     4081 ttaaacagct caagcctgct ttggtcagaa ttgaaatccg ttcatccctt ccgccaaaca
     4141 cataatgcac ttgctttttg cgattcactt atcactaccg atactgaatt cagatcgtct
     4201 ttttccggca aacccgtgtt attttctgtt caccctgccg gaaataaggg cattgaaact
     4261 atgcttgcca ttgagctccc ctccggtgga gagaaaaaat gcctatcctt gcttgagcga
     4321 gtgcttaaac aaactttttc ggttcgcgaa ttcaaatcag aatccattta caacctaacc
     4381 ctcggaaatg ctccctggca aatattaatc aaagagaatt ttctgacaat aagctcaagc
     4441 gcaacacttg ccgaacgtat ttccatgcaa ctggatcagg atatctcaat tgccaccgaa
     4501 gccggttgga aagaagtata tgccacttcc gatatcggtg cacaggccaa tatctttatc
     4561 catcatcaga atttttccaa attgattcat gctctggtcc aagaggacca aaacgatgct
     4621 ttaacaggga tttatcctta tgcctatttt acagaattcg acctgagtct tcgttcaggt
     4681 accgtttctc ttaatggttt ctcctttgct cctgatagtt taggatcaat gctgaatatt
     4741 ttttcaaaac atgaaccaca accttttgaa gtcgcacgct atttaccgga taacacagct
     4801 gcatttttgt ggttgggaat taatgatggg gaaagtttca gaacttctat tgatgcctat
     4861 cgtcaatcaa aaggaacctt acagcaatac cgaaaggaaa tcaatagttt caatgccgat
     4921 aaagattgca atatagagcg ccagctggtt tcatggatag gcaatgagat cgttctcttt
     4981 agcaatggaa aagctgatac cagtgctaaa aaagaaaacc tttttcttgc cattcgcatc
     5041 aatgatatgg cagatccaat aagcgaattg aatgcgcttg cagataaact cgacaccaat
     5101 gctacacgca ttcgtgatca ggatggaatg caaatgcgca gactgcttgc caacgatctt
     5161 ttttcctttt tgttcggacc tgcatttgat ggcatcgaca acccctggta cattagaatt
     5221 gaagactata tgatctttgc aaactcggga gaggctttga aacaatattt aatggatatc
     5281 agctccgatc gcacccttgg aaaaaacatc acctactaca atttcgtatc tgaaaacctt
     5341 tctgatcgag ccaatctttc agtgtatgtt aatccctcga aattcaaatc cttgttgcct
     5401 cagttcttta ataacaccac cagtgaagaa cttgaaaatc cgaattcctt actctggaaa
     5461 tttgatgctc ttgcatggca gttcagcaat tccggtaaag gaatgttcta caacaaccta
     5521 tatctgaaat acaacggaac aggtaaacaa gattcaaaat ctctttggga agttgctgta
     5581 gatactaccg taaacatgca accacttctg gtgaaaaacc acattacagg aacaatagat
     5641 gttgttgtac aggatgagaa ttacagcttc tatctgattt ccagcaaagg agaaattctt
     5701 tggaaaaaac aacttgagga acgcctgaca ggtgatgtgc gccagatcga cttcttcggt
     5761 aatggtaaat tacaattgct ctttgcaacc actacagcaa ttcacctgat cgatctcaaa
     5821 ggaaattacc ttaaaggttt cccctaccgt ttgaaagaag aagtaagtgg tcctcttggt
     5881 gtattcgatt acgaatcgaa caacaattac cgtattctta ttccttgcgg aaggaacatt
     5941 gtaaacatcg ataaagaagc taaagtcaca accggatggg aacctgaaaa attctcctcc
     6001 agtattaacc aggctcctgt tttcttccgt tgcgacaata aagattattt gtttgtaatt
     6061 gatacaaaag gggagattcg catcatcgac cgcaaaggaa aaccacgcta caacctgagc
     6121 tttggtttgg aaaatcgttc gtccaataaa gtgttactcg aacaaggatc aagcattgag
     6181 ggcacatctc tcatttactc cgacaccagc ggaagaatca ttcgcacttt ccttaacggc
     6241 aatgccgata ccgcacgaac aggattaaaa agttcaagtc accgcttcgc atatatcgat
     6301 gccaatgaag atggaaaaaa agatattgtg ctacttgact ccaataaaat tgacatcaca
     6361 gaatggtccg gaaaaagaat tggtgtgtat catttccctg agcgagtgag ttcgcacttg
     6421 atggtatttg ctctaaaaga cggttccatt cgaattggag cagttgcccc gggtgttgga
     6481 gaaatctatt tactgaacag cagcggacaa cttcacagca agtttcctct taccggaacc
     6541 acagcttttc ccttaagcga catcaatggg gacgggagtc ttgaggtagt ttcaggtacc
     6601 ggatcaagac gaattgtctg ttattcactt aattaaaaca aaaaaaccgt aaattaattt
     6661 ccggttgaaa cgaatatgcc attacgaagg gattacctat ttttggttaa ttctttcgat
     6721 atgaaaaaaa tactagttgc acttttagtt gtgcttaacg ttatctccgt ttcagcacag
     6781 gatttccttg gctttgtcaa ca
//
LOCUS       DAOMYG010000134         6798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3811_length_6798_cov_9.439122, whole genome shotgun sequence.
ACCESSION   DAOMYG010000134 DAOMYG010000000
VERSION     DAOMYG010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6798
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3811_length_6798_cov_9.439122"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..718
                     /locus_tag="PK610_05710"
     CDS             2..718
                     /locus_tag="PK610_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRJ35461.1"
                     /translation="MRLSYVFRLFAPLFLKGNKVECTVCKKHYRKFLPYGTHQRDNVL
                     CPNCLTLERHRLFWLYLNEKTDFFTAKHEVLHIAPEQCFYKRFRSMSNLRYVTADLES
                     PLADVHMDMHHMPFKNEEFDIVFANHVLEHVDDPHQCMTEVLRVLKPGGWAIMQVPID
                     YSRETTYEDSSITSPADREKHFWQKDHLRLFGRDYPERLRKAGFSVDENNFSAALPDD
                     VRERFRLQKEEVLYICKKIT"
     gene            809..2287
                     /gene="guaB"
                     /locus_tag="PK610_05715"
     CDS             809..2287
                     /gene="guaB"
                     /locus_tag="PK610_05715"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRJ35462.1"
                     /translation="MTITKDKFIGEGLTYDDVLLVPAYSEVMPREVDIRSKFTKGITI
                     NLPVVAAAMDTVTESELAIALAQQGGIGVIHKNMSIEAQANEVRKVKRSESGMIQDPI
                     TLQVNATVSDALKLMAENKIGGIPVVDANQKLVGIVTNRDLRFEKVMTRPVAEIMTSS
                     NLITAKAGMDLRSAEGILQSNKIEKLPVVDQEGKLVGLITYRDIIKIKEHPNSCKDQY
                     GRLRVAAAVGVTGDTLDRVSALVAAGVDAITIDTAHGHSKGVIEVLKKVKASYPDLQV
                     IVGNIATGEAAKALAEAGADAVKVGIGPGSICTTRVIAGVGVPQLSAVMMVAEALKGT
                     GVPLIADGGIRYTGDVVKAIAAGASTVMVGSMFAGVEESPGETIIYEGRKFKSYRGMG
                     SIEAMQQGSKDRYFQDMEDDIKKLVPEGISGRVPYKGRLTEVTYQLIGGLRAGMGYCG
                     SKDIPTLQEKSKFVRITMAGFRESHPHNITITREAPNYSYNQ"
     gene            2308..4260
                     /locus_tag="PK610_05720"
     CDS             2308..4260
                     /locus_tag="PK610_05720"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRJ35463.1"
                     /translation="MKTRHILGIFLAILFAVSASAQTDPVLMWVNEKPIHKSEFEAIF
                     RKNQKETGVSREALDEYLVLFTNFKLKVTEAEELGMDTVSKFKEELAGYRKQLARPYL
                     VDNEMTDKLIAEAYERMKFELRASHILVKCDIDAEPKDTLIAYQKALKFRERILKGED
                     FGKAASVKGGSDDPSAIKNKGDLGWFTAFMMVYNFENACYNMNLGDLSMPVRTRFGYH
                     IIKLTGKRPARGTIRVAHILIASKDEDSNEAKAQAKKKAEEILGRIQAGGDFGTLANQ
                     FSEDPSSKGKGGELSPFSTGRMVEEFEEASFALNKDGDVSGIVKTAYGYHIIKRLELK
                     PIDSFEEMKATLKQKVQRDVRSQLPRKSFVAKLKTKYNFKEDKAALKPFFTVVDTNIF
                     YGKWDATKAAKLNKFMWSFNGKNYTQADFTNFLNKSQRRTQREDVSNFVAKQYEKWTS
                     DLIIDFEDARLEEIYPDFRMLMKEYRDGILLFELTDQKVWTKAIKDSVGLASFYEKNK
                     NNYLWQKRYVVDIFYCANAKIAKALRKDIAKGKMSNEALLEKYNKESQLNLKIESGKY
                     NEEENVTLKTNLPKAKGLSADIQLNGQVVIINAKEIIEPTPKAINEAKGLITSDYQSY
                     LEKEWLEQLRKKYPVRVDNTVLYSIK"
     gene            4267..5106
                     /locus_tag="PK610_05725"
     CDS             4267..5106
                     /locus_tag="PK610_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRJ35464.1"
                     /translation="MLRFLLPVFVFVACSSGKGEKEENVLATVYDKNLTLDKVLPLIP
                     EGINPEDSVAFVKGFVESWIRDMVIVHQAEKTLPEETLNVEARLENYRRSLLVYAYEQ
                     EYARQKLDTIVSDEEIEAHYEANKDDFRLADYIVKVLFVKLVRDQPGKANVAKWYKST
                     QQKDLMELEKFCVENAVSFYNDSESWIYLSDLMQEIPIRTDDRATFLKSTQKLEFEDE
                     EFAYFLLIHDYRLKDALSPLSFERENIKSRILNARTNEIVRKMRTDLLNSSQSQIRNY
                     VEN"
     gene            5093..6442
                     /locus_tag="PK610_05730"
     CDS             5093..6442
                     /locus_tag="PK610_05730"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRJ35465.1"
                     /translation="MLRIKRLFSVAGLFLTIQAAGQGKNIDRIIAVVGDRPILFSEVE
                     MQRMQAVAQGLPFGPEKRGEILDEILFQNLLITHAELDSLTVSDAQVTNEMEGRLRYF
                     EQQIGGRKKLEEFYGKSIAQIKEEFFDMIKDKLMAQAMERKITEGIKVTPNEVRAFYN
                     RIPNDSLPLINSMVELAQLTIVPKVSDEEKKRTRIKLEAVRKRIMSGASTFCVEAIDS
                     DDPGSNGNCGEWDFIPRGTFVPQFDAIAFRLKDGEFSEVFETDYGFHFMQLIQRRGDE
                     YKGRHILFVPKISPLEMKASSLRLDSLYQAIRDGKISWEKACEKYSTDAESKRNGGRM
                     MNPMNGDTKFDVADLDPQLFLTIDKMKPGQISLPVGYTTQDGKPGFRLLKLISRSAPH
                     QANLTDDYQLIQNAAQNDKESKTVDDWARNKITSTYVWIADDFKQYTYRYNWIRHNN"
     gene            6442..>6798
                     /locus_tag="PK610_05735"
     CDS             6442..>6798
                     /locus_tag="PK610_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35466.1"
                     /translation="MLAVPLVSLDPYKRAKIWYAAGFVVAGLTLLFFSFTANPSKDQA
                     MVLGGLSLAFLLTGAFLYLRAGKIHLCGTFKLNGTSLIVERLDHSLEQSLKEMGSVRF
                     TDEGFHKLHGNHGKIFG"
BASE COUNT         2066 a         1288 c         1610 g         1834 t
ORIGIN      
        1 aatcagatta agttatgtgt ttcgtttgtt cgctcctttg tttttaaagg gcaacaaagt
       61 tgagtgtacg gtatgtaaaa agcactaccg taaatttctg ccctatggaa cgcatcaaag
      121 ggacaatgta ctttgtccga attgcctgac tctggaaaga catcgtttat tctggcttta
      181 tctgaatgaa aagacggatt tctttacagc taagcatgaa gtattgcata tcgctccaga
      241 acaatgtttt tacaagcgtt tccgttccat gagcaacctt cgttatgtta cggccgatct
      301 tgaatctccc ttagcagatg tgcatatgga tatgcatcac atgccattta aaaacgaaga
      361 attcgatatt gtatttgcca atcacgtttt ggaacatgtt gatgatccgc atcaatgtat
      421 gacggaagta ttacgtgtgc ttaagcccgg aggatgggca attatgcagg tccctattga
      481 ttactccagg gaaacgacct acgaggacag cagcattaca tctcctgccg accgcgagaa
      541 gcatttttgg caaaaggatc atttgcgcct gttcggaaga gactatcctg aacgcctgcg
      601 caaagccgga ttttcagttg atgaaaacaa tttttccgct gctcttcctg atgatgtacg
      661 cgagcgtttc cgcttgcaaa aggaggaggt tctttacatc tgtaaaaaga tcacgtagtt
      721 aaatcttctt aacaaccgaa gcctcagttt ccaatcatag aatgctcctt tttcctatct
      781 ttgcccaatt ttaattgaag gagcgaatat gacgatcaca aaggacaaat tcattggcga
      841 agggctgaca tacgatgatg ttttactggt acctgcttat tcggaggtta tgccccgcga
      901 agtagatatc cgttctaaat tcacaaaggg aataacgatc aaccttccgg tagttgctgc
      961 tgcgatggat actgttactg agtctgaact ggcaattgcc cttgcccaac agggtggaat
     1021 aggggtgatt cacaaaaaca tgagcatcga ggcgcaagcc aacgaggtac gtaaggtgaa
     1081 acgatcggag agtggaatga ttcaggatcc gatcacattg caggtaaatg ctacggtttc
     1141 tgatgctttg aaactgatgg cagaaaataa aattggcgga attcccgttg ttgatgcaaa
     1201 tcaaaaactc gttgggattg taaccaatcg cgatctccgc tttgagaagg tgatgacacg
     1261 tccggtcgct gaaatcatga ccagctccaa tctgattaca gccaaggcag gaatggatct
     1321 acgcagtgct gaaggtattc ttcaatccaa taaaatcgag aaattacccg ttgttgatca
     1381 ggaaggcaaa cttgtaggct tgatcactta tcgcgatatt atcaaaatca aggaacatcc
     1441 caattcctgc aaagatcaat acggaagact tcgtgttgct gctgcagttg gtgttacagg
     1501 agatacactc gatcgtgtat ctgcattggt tgcggccgga gtagatgcaa ttaccatcga
     1561 tactgcccat ggtcattcca aaggggtgat tgaagttttg aaaaaggtaa aagcaagcta
     1621 tcccgatctt caggttattg tggggaatat cgctaccggt gaagcagcca aagcattggc
     1681 cgaggcagga gctgatgcgg tgaaggtggg tattggtccg ggttctattt gcaccacacg
     1741 tgtaattgca ggggttggtg taccacaatt atcggcagtg atgatggttg ccgaagcact
     1801 caaggggaca ggtgttcctc tcatagcaga tggtggaatt cgttataccg gtgatgtggt
     1861 taaagcaatt gctgcaggtg catctactgt tatggttggc tccatgtttg caggagtgga
     1921 agagtcgccc ggagaaacca ttatttacga aggaaggaaa tttaaatcct atcggggaat
     1981 ggggtcgatt gaagctatgc agcaaggttc gaaggatcgc tatttccagg atatggaaga
     2041 tgatattaaa aaactggttc ctgaagggat ttcaggacgc gtcccctaca aaggtcggct
     2101 tactgaggtc acttaccaac tgattggagg attacgcgca ggaatggggt attgcggatc
     2161 gaaagatatt cctacgcttc aggaaaagag caaatttgtg cgaatcacca tggcaggatt
     2221 cagagagagt catcctcaca atattacgat tactcgggaa gcacccaatt acagttataa
     2281 tcaatgatcg ttaacaatag caacaccatg aaaacacgtc acattctcgg aatttttctt
     2341 gcaattctgt ttgcagtatc ggcaagcgca cagaccgacc ccgtgcttat gtgggtcaat
     2401 gaaaaaccca tccataaatc cgaatttgaa gcgattttcc gcaagaatca aaaagaaacc
     2461 ggtgttagtc gtgaggcact ggatgagtat ctcgtattgt tcaccaattt caaactgaaa
     2521 gtaaccgaag ccgaagaact cggaatggat accgtttcga aattcaagga agaacttgca
     2581 ggatacagaa aacaacttgc gcgtccttat ctggtagata atgaaatgac cgataaactc
     2641 attgcggagg cctacgaacg aatgaaattc gaattgcgtg ccagtcatat tcttgttaag
     2701 tgtgatatag atgcagagcc taaggataca ctgattgcct atcaaaaagc attgaaattc
     2761 cgtgagcgca ttctcaaagg agaagatttt ggaaaagctg catctgttaa aggtgggtca
     2821 gatgatccga gtgccattaa aaacaaagga gacctgggat ggttcactgc gtttatgatg
     2881 gtttataatt ttgaaaatgc atgttacaac atgaacttgg gagatctgtc gatgccggtg
     2941 cgtacgcgtt tcgggtatca catcatcaag ctcacaggaa agcgtcctgc tcggggtacc
     3001 attcgtgttg cgcatattct gattgcatca aaggatgaag attccaatga agctaaggca
     3061 caggcaaaaa agaaagcaga agaaattctt ggccgtattc aggcaggtgg tgattttgga
     3121 actttggcca atcagttttc cgaagatcca agttcaaaag gaaaaggtgg agaactttct
     3181 cccttcagta caggtcgtat ggttgaggaa tttgaggagg cttcatttgc attgaacaaa
     3241 gacggtgatg tttcgggaat tgttaaaacc gcttacggct atcatatcat taagcgactt
     3301 gaacttaagc ctatcgattc attcgaagaa atgaaagcaa cgctcaagca aaaagtacag
     3361 cgtgatgttc gctctcaact tcctcgtaaa tcgtttgtgg ctaaactgaa aacgaaatac
     3421 aacttcaagg aagataaagc tgcattgaaa cccttcttta ctgtggtgga tacgaatatc
     3481 ttctacggaa agtgggatgc aaccaaggct gccaagctga ataaattcat gtggtcgttc
     3541 aatggtaaga attacacaca agccgatttc accaatttcc tgaataagag tcagcgcaga
     3601 acacaaaggg aagatgtttc caattttgtg gctaagcaat atgaaaaatg gacttccgac
     3661 ttaatcatcg attttgaaga tgctcgtctt gaagaaattt accctgattt ccgaatgctg
     3721 atgaaagaat accgcgacgg aattttattg tttgagttaa cggatcaaaa agtatggaca
     3781 aaagccatta aggattctgt tggacttgcg agcttctacg agaaaaataa aaacaattac
     3841 ctctggcaaa aacgttacgt agttgatatt ttttattgcg ccaatgctaa aattgcaaaa
     3901 gcccttcgca aggatatcgc taaaggaaaa atgagcaatg aagctttgtt ggaaaaatac
     3961 aataaggaat cacaactcaa cctgaagatt gaaagtggta aatacaacga agaagaaaat
     4021 gtaacgctga aaaccaattt gccaaaagca aagggtcttt ctgccgatat tcaattgaat
     4081 ggacaggttg ttatcatcaa tgccaaggag attattgaac caacacctaa ggccattaac
     4141 gaagcgaagg gattgattac gtcggattat cagtcttacc tggaaaagga atggctggaa
     4201 caattgcgta aaaagtatcc ggttcgtgtt gataatactg ttttatacag cataaaataa
     4261 tccgggttgt tacgatttct gttgccggtc ttcgtttttg ttgcttgttc ttccggaaaa
     4321 ggagagaagg aagaaaatgt gttggccaca gtgtacgata aaaatctgac gctcgataaa
     4381 gttctaccgc ttattccgga aggaattaat ccggaagaca gtgtggcatt tgtgaaggga
     4441 tttgtggagt cctggatacg cgatatggta atcgtacatc aggcagaaaa aacgcttccg
     4501 gaagaaacac tcaatgtaga agcgcgactg gaaaactacc gtcgctcctt gctggtttat
     4561 gcctacgaac aggagtatgc gcgtcagaaa ttggatacga ttgtaagcga tgaagaaatt
     4621 gaagcacatt atgaggccaa taaggatgat tttcgtttgg ccgattacat tgtaaaagtg
     4681 ttgtttgtaa aactggtccg cgatcaacct ggtaaggcca atgttgccaa atggtacaaa
     4741 agcacacagc aaaaagattt aatggagttg gagaaatttt gtgtggaaaa tgccgttagt
     4801 ttctataatg attcggaaag ctggatttat ttaagtgacc tgatgcagga gattccaata
     4861 cgcaccgacg atcgtgcaac atttttgaag tcgacccaaa agctggagtt tgaggacgaa
     4921 gagtttgcct attttttgct gattcacgat tatcgcttaa aagacgcatt gtcgccatta
     4981 agttttgaac gagaaaatat taaatcgagg attttgaatg cccgtaccaa tgagattgtg
     5041 cgaaagatgc ggacagatct tttaaactcc tctcaatcac aaatacgaaa ctatgttgag
     5101 aattaagcgc ctattcagtg ttgcaggttt gtttctgacc attcaggcag cagggcaagg
     5161 caagaacatc gaccgcatta ttgcagttgt tggtgaccgt cctattttat tttctgaggt
     5221 agaaatgcaa cgcatgcagg ctgtagcgca aggattgcca tttggccctg aaaaacgtgg
     5281 tgaaattctg gatgaaattt tatttcagaa tttattgatt acccatgctg aattggactc
     5341 gcttacggta agtgacgctc aggttaccaa cgaaatggaa ggtcgtctgc gttattttga
     5401 acaacagatc ggaggtcgca aaaagctgga agaattttac ggcaagagca ttgctcaaat
     5461 caaggaagag ttctttgata tgatcaagga taaactaatg gcgcaggcta tggagcggaa
     5521 aattacggaa ggaataaaag taactcccaa cgaagttcgc gcattttaca accgtattcc
     5581 caacgacagt cttccactaa taaattcaat ggtagaactt gctcagttaa ccatagtccc
     5641 aaaagttagt gatgaggaaa agaaacgcac acgcattaaa ctagaggccg tccgcaaacg
     5701 gatcatgtct ggtgccagta cgttctgtgt agaagcgatc gattcggatg atccgggttc
     5761 aaatggtaat tgcggagaat gggatttcat tcctcgcgga acatttgtcc ctcaatttga
     5821 tgccattgcc tttcgtttga aagacggtga gttttctgaa gtatttgaaa cggattatgg
     5881 ttttcacttt atgcaattga ttcagcgaag aggagatgaa tacaaggggc gtcatatcct
     5941 ttttgtacct aaaatttctc ctttggaaat gaaggcatcc agtttgcgac tggattcgct
     6001 ctatcaggca attcgcgatg ggaagataag ctgggaaaag gcatgtgaga aatattctac
     6061 agatgcagaa tccaaacgaa acggtgggag gatgatgaat ccgatgaacg gagatactaa
     6121 atttgatgtt gccgatctgg atccgcaact gtttctgact atcgacaaaa tgaaaccagg
     6181 acaaatctca ttgccggtag gctataccac ccaggatgga aaaccgggat tcaggctgct
     6241 aaaattaatc agcagaagtg ctccgcatca ggcgaattta acagacgatt atcagctgat
     6301 acagaacgct gcccagaacg ataaggaatc gaagacagta gatgattggg ccagaaataa
     6361 aatcacctct acctatgtgt ggattgctga cgattttaag caatacacgt atcggtataa
     6421 ttggattcgc cataacaact gatgttagcc gttccacttg tatctcttga tccctataag
     6481 cgagccaaaa tatggtacgc tgccggattt gttgttgccg gattaacctt gttgtttttt
     6541 tcctttactg ccaacccaag taaggatcag gcgatggttt taggtggatt gtcgctagct
     6601 tttttactta ccggtgcatt cctttatctg cgtgcaggaa aaattcattt atgcggaacc
     6661 tttaagctca atggaacaag tttgattgta gaacgtcttg atcattccct cgaacaatca
     6721 ttgaaggaaa tgggaagtgt acgttttacc gatgaaggat ttcacaagtt gcatggaaac
     6781 catggaaaga ttttcggg
//
LOCUS       DAOMYG010000135         6793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3815_length_6793_cov_7.643811, whole genome shotgun sequence.
ACCESSION   DAOMYG010000135 DAOMYG010000000
VERSION     DAOMYG010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6793
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3815_length_6793_cov_7.643811"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="PK610_05740"
     CDS             complement(<1..1297)
                     /locus_tag="PK610_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRJ35467.1"
                     /translation="MAGKEKKESGLWEYSPAPESASIGKIAPKYGLFIDGKFIAPAKG
                     RYFPSINPATEAKLSMIAEADAADVDKAVKAARKAFGSWSRLSASERGKYLYRIARMI
                     QERAREFAVIETLDGGKPIRESRDIDIPLAAAHFFYYAGWADKLEYAFPNRKPKPIGV
                     AGQIIPWNFPLLMAAWKIAPALAAGNTVVLKPAETTSLTALKLAELFSDCGLPPGVVN
                     IVTGAGATGAALVNHKGIDKIAFTGSTDVGKIIQKAIAGTTKKSTLELGGKAANIIFE
                     DATIDQAVEGIVNGIYFNQGHVCCAGSRLFVQESIHEEVVRKLKHRIKSLRVGDPMDK
                     NTDVGAINSKAQLETIQKYVRLGKEEGAEIFQPSCNLPDKGFWCPPTLFTNVSQSHTI
                     AREEIFGPVLAIQTFRTEAEVIAKANNTPFGLSAGVWTDK"
     gene            complement(1300..2214)
                     /gene="deoC"
                     /locus_tag="PK610_05745"
     CDS             complement(1300..2214)
                     /gene="deoC"
                     /locus_tag="PK610_05745"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRJ35468.1"
                     /translation="MTQRKISGIPATSPRIDQVGVEERCARFTSRSIKSTTKMQGLHM
                     VLSMIDLTTLEGADTEGKIRQMCYKASHPHESIKGLPATAAVCVYPTMVGVAKKALQG
                     TGVNVASVSTAFPSGQGPTEVKLADTKLALDFGADEIDMVISRGRFLQGEEQFVYDEI
                     AMIKELCGKKARLKVILETGELGTLDRVRRASDIAIAAGADFIKTSTGKISPAATMPV
                     TLVMLEAIRDHYFSTGEMVGMKPAGGISSAKSALHYLVMVKETLGEEWLNKDWFRFGA
                     SRLANDVIMQIAKQTTGNYQSGDYFSKD"
     gene            2355..2960
                     /gene="cysC"
                     /locus_tag="PK610_05750"
     CDS             2355..2960
                     /gene="cysC"
                     /locus_tag="PK610_05750"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HRJ35469.1"
                     /translation="MSGKGNHIFPVSDRLLQRSDKESLLNQKGLVVWMTGLSGSGKST
                     IAIGLEKRLHQDGILVKVLDGDNVRTGINNNLGFSESDRKENIRRIAEVSKLFVDCGI
                     VCINCFVSPTKEIRQQAKDIIGGTDFYEVFINTPLEICEQRDVKGLYKKARAGEIADF
                     TGISSPFEPPVIADIEIQTKGKPVEKSVEELYQFIIKKIKS"
     gene            2963..3868
                     /gene="cysD"
                     /locus_tag="PK610_05755"
     CDS             2963..3868
                     /gene="cysD"
                     /locus_tag="PK610_05755"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015754623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HRJ35470.1"
                     /translation="MAGYHLTHLRELEAESIYVLREVAAQFENPALLFSGGKDSIVCF
                     HLAQKAFWPAKIPFTLVHIDTGHNFQETLDFRDELVKKNNVNLIVGSVQESIDAGKAT
                     EEKGINASRNALQTVTLLDTIEKYKFDAAIGGARRDEEKARAKERFFSHRDEFGQWDP
                     KNQRPELWNLFNGKKNIGEHFRVFPISNWTEMDVWQYILQENIEIPSIYFTHERDCFV
                     RDGVIYAHSSFMQLKPTEKVERRTVRFRTIGDMTCTGAVVSEASTLSQIIEEVASTRV
                     TERGSRIDDKRSEAAMEDRKKAGYF"
     gene            3878..5161
                     /gene="cysN"
                     /locus_tag="PK610_05760"
     CDS             3878..5161
                     /gene="cysN"
                     /locus_tag="PK610_05760"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HRJ35471.1"
                     /translation="MNNLPTDNLSGYLDMDLLRFTTAGSVDDGKSTLIGRLLYDSKSI
                     FEDQMEAIERASQKKGEEHVNLALLTDGLRAEREQGITIDVAYRYFATPKRKFIIADT
                     PGHIQYTRNMVTGASTANLAIILVDARKGIVEQTCRHSFIASLLQIPHIVYCINKMDL
                     VDYDKDTFFRIKKELEDFSAKLDVRDINFIPISALNGDNVVDRSTKMNWYDGQTLMYL
                     LENVHIAGDYNHIDARFPVQYVVRPMTTEHHDYRGYAGRVASGVLRVGDDITVLPSGF
                     ESKIKSIDTSTGMLEEAFAPMSVCLTLEDEIDISRGDMIVRRNNLPEQSQDVEAMVCW
                     FTERPLQTGGKYAIKHTSKDVRCMIKDIRYKMDINTLHRNEEDKNIKMNDIARVVLRT
                     TAPLFTDKYHRNRFTGSFILIDEGTNETVGAGMII"
     gene            5191..5994
                     /locus_tag="PK610_05765"
     CDS             5191..5994
                     /locus_tag="PK610_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35472.1"
                     /translation="MRKSSLLLLFGFLSIHSCRRADRDLDRDISETKDQVFGTYVWLD
                     VFSRVSEACSFEPGLRMLECGNVMIDTLSSPMSILIDFGPTDCTGNDGRTRSGKIFAS
                     LSSGYNDSATIVQVQLQNYRFNGQNIQGQMTITNNGRDTQSRRKFAVSVQQSTIQFSA
                     SNRTSIWSSSLTLLLNSGETTVAISDDSFLVSGTFSGKSSKGNLYTGNISQPISYQLS
                     CGEIVSGSIDVLPENLNKRVALFGDGNCDAIILVNVNGFEEEFNPESID"
     gene            complement(5991..>6793)
                     /locus_tag="PK610_05770"
     CDS             complement(5991..>6793)
                     /locus_tag="PK610_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35473.1"
                     /translation="LLFGKPQKALELLQNEWDRFKEYQIPERSVFSETMMTKLGLLYL
                     RCAIAASKSSIASEAIMVLRLSKHDVNSTLEFKALEMEYHMNSGNLDQACRMEPEAVE
                     MLWKDESGTTNLLENNLAFQCLRTCFFSGRTKEFNAMANRLVAGKDACKDSLFFKVYP
                     LLILSALDQGEAMLVRSYTATYSRKLSQCNCLNTPEITFLEMVRKLNGKTPFDRASTM
                     NRYFNAFSLLCSDPVEAAIVQSLDVFKWMESIHNIADGKQLSPVKIAV"
BASE COUNT         1996 a         1447 c         1401 g         1949 t
ORIGIN      
        1 ctttatctgt ccatacaccc gcagacaatc cgaatggtgt gttattcgct tttgcaataa
       61 cctcagcctc tgttctgaat gtctgtattg ccagcacagg tccaaatatt tcttcccggg
      121 caattgtgtg tgattgagaa acgtttgtaa ataaggttgg cggacaccag aaacctttgt
      181 cagggaggtt gcaggaaggc tgaaaaattt cggcgccttc ttcttttcca aggcggacat
      241 atttttgaat ggtttccaat tgcgctttcg aatttatggc tcctacgtct gtgtttttat
      301 ccattggatc accaacacga agggacttaa tgcgatgttt taactttcga actacctctt
      361 catgtatact ctcctgaaca aacaaacgtg aaccggcaca acaaacatgg ccctggttga
      421 aatatattcc atttactata ccttcaacag cctgatcgat agttgcgtct tcaaaaatga
      481 tgtttgcagc ttttcctcca agctcaagag ttgatttttt tgttgttcca gcaatagctt
      541 tctggatgat tttgcccaca tcagtagagc cggtaaatgc gattttatca atgcctttat
      601 gattcaccaa tgccgcacct gtagcgcctg cgcctgtaac aatgttaact acaccgggag
      661 gaaggccgca gtcagaaaac aattctgcca atttcaaggc tgtaagtgat gttgtttccg
      721 ctggctttaa aaccactgtg tttcctgctg ccaatgccgg agcaattttc caggcagcca
      781 ttaacaaagg aaaattccag ggaatgatct gaccggctac accaattggt tttggttttc
      841 tgttgggaaa agcatactcc agtttgtctg cccaacctgc ataatagaaa aagtgcgcgg
      901 cagccagagg aatgtcaata tcacgacttt cacgaatggg tttcccgcca tccaatgttt
      961 ctatcactgc aaattcacgg gcacgctcct gaatcatgcg ggcgatgcgg tacaaatatt
     1021 ttccacgctc cgatgcagaa agtcgcgacc aggatccaaa tgcctttcgt gcagccttca
     1081 ccgctttgtc aacatctgca gcatctgcct cggcaatcat gctcagcttt gcttcagttg
     1141 ccgggttaat acttgggaag tatcgtccct ttgccggtgc tataaacttt ccgtcaatga
     1201 acaggccata ctttggtgca atttttccaa tagaagcact ttcgggtgca ggggaatact
     1261 cccacaaacc tgattctttt ttttcttttc ctgccatttt cagtcttttg aaaaataatc
     1321 tccggattga taatttcctg tggtttgttt tgcgatttgc atgatcacat catttgcaag
     1381 gcgactggca ccaaacctaa accaatcttt attcaaccat tcttcgccta gcgtttcttt
     1441 taccatcacc aaatagtgta aagcagattt tgcgcttgaa attcctccgg caggcttcat
     1501 gccaaccatt tcaccagtac tgaaataatg atcgcgaatg gcttccagca ttaccaaagt
     1561 taccggcatg gttgcagcgg gtgatatttt tcctgttgat gttttaataa aatcagcccc
     1621 agcggcaatc gcaatgtcac ttgccctgcg aacacgatca agtgtgccca actcgcctgt
     1681 ttccaaaatc acttttaatc gggctttctt tccgcacaat tcctttatca tagcaatttc
     1741 atcatataca aactgctctt ctccctgcaa aaaacgaccc ctggaaatta ccatgtctat
     1801 ttcatccgca ccaaaatcca acgcaagctt ggtgtctgcc aatttaactt ctgtgggccc
     1861 ttgaccggaa ggaaaagctg tagatacaga agcaacatta acacctgttc cctgcagcgc
     1921 cttttttgcc actccaacca ttgtagggta cacacaaact gcagcagtgg caggcaagcc
     1981 ctttatgctt tcgtgaggat gtgaggcctt gtaacacatc tggcggattt tcccttcagt
     2041 atcagcacct tccaaagtgg ttaaatcaat catgctcagc accatgtgta gcccttgcat
     2101 ctttgttgtc gatttaatac ttctggaagt aaaacgtgca cacctttcct cgactcccac
     2161 ctgatcgatg cggggtgaag ttgccggaat gcctgaaatt tttcgttgtg tcataggcag
     2221 caaaatgtac tgaaatcaaa tatagaatta ataattcact aagcgattac cataaaatcg
     2281 gacttaaaaa tcgaatagga caattcagca ctttccttgc gctaccagtt gtttattagt
     2341 gtaaatttgt gattgtgtca ggaaaaggca accatatttt cccggtttca gaccggctgc
     2401 ttcaacgatc ggataaagag tcgttactga atcaaaaggg ccttgtggtt tggatgacag
     2461 gattatccgg gtctggcaaa tcgaccattg ccattggttt agaaaagcgc ctgcatcaag
     2521 atggaattct tgtaaaagtt cttgacggcg ataatgtcag gaccggtatc aataataatc
     2581 ttggtttttc cgaatccgac cgaaaagaga acattcgaag aattgctgaa gtatcaaaac
     2641 tgtttgttga ttgtggaatt gtctgcatca attgttttgt tagcccaacc aaagaaatac
     2701 gacaacaggc aaaagatatt attggcggca ctgactttta tgaagttttt ataaataccc
     2761 ctcttgaaat ttgcgaacaa agagatgtaa aaggacttta caaaaaagca cgtgccggag
     2821 agattgcgga ttttacaggt ataagttctc cttttgaacc gccagtaata gcagacatcg
     2881 aaatccaaac caagggaaaa cccgttgaaa aatctgttga agaactgtat caattcatta
     2941 ttaaaaaaat caaatcctga ctatggcagg ctaccattta actcacctga gagaattaga
     3001 agctgaaagc atttatgtgc ttcgtgaagt tgccgcgcaa tttgaaaacc ctgccttatt
     3061 attttccgga ggcaaagaca gcattgtctg ttttcatctt gcacaaaaag cattttggcc
     3121 ggcgaaaatt ccgtttacgc ttgtacacat tgataccggg cacaatttcc aggaaaccct
     3181 tgatttcagg gatgagctgg tgaagaaaaa caacgtgaat cttattgttg gcagtgttca
     3241 ggaatcgatc gacgcaggga aagcaacaga ggaaaaaggt attaatgcca gcagaaatgc
     3301 gcttcaaaca gttactctgc tcgataccat tgaaaaatat aaattcgatg ctgccattgg
     3361 gggggctcgt cgcgacgaag aaaaagccag agccaaagaa cgctttttct cacatcgtga
     3421 tgaatttgga caatgggatc caaaaaatca gcgaccagaa ctttggaatc tgttcaatgg
     3481 caaaaaaaat atcggagaac attttcgcgt gtttccaatt tccaattgga cagaaatgga
     3541 tgtttggcaa tatatcttgc aggaaaacat agaaattcca tccatctact ttactcatga
     3601 acgcgattgt tttgttcgcg atggagttat ttacgcgcat tcatcgttca tgcagttaaa
     3661 gccaaccgaa aaagttgaac ggagaactgt tcgttttcgg actattggcg atatgacttg
     3721 tacaggagca gttgtatcag aagcatccac cctttcacaa atcattgaag aagttgcctc
     3781 gacaagagtt accgaacgcg gatcaagaat tgacgacaaa agatctgaag ccgcaatgga
     3841 agaccgcaaa aaagcaggat atttttaacc gatcatcatg aataatttac ctacagataa
     3901 tttaagtgga tacctcgata tggacctcct tcgctttaca acagcaggca gtgttgacga
     3961 tggaaaaagc accttgatcg gcagattgct ctatgattct aaatccattt ttgaagatca
     4021 gatggaagca atagagcgtg ccagtcagaa aaaaggagaa gaacatgtaa atcttgcttt
     4081 actcactgat ggtttacgtg cagagagaga gcagggaatt accattgacg tcgcctaccg
     4141 ctattttgcc acaccaaaac gcaaattcat tattgcagat actccgggac atattcagta
     4201 caccagaaac atggttacag gagcgtctac ggccaatctt gcaattattc tggtggatgc
     4261 cagaaaaggc attgttgaac aaacgtgtcg tcactcattc attgcttcac ttttacagat
     4321 cccccatatt gtgtattgta tcaataaaat ggatctcgtt gactatgaca aggacacctt
     4381 tttcagaata aaaaaggaat tggaggattt tagcgcaaag cttgatgttc gtgatattaa
     4441 ttttatcccc atttcggctc ttaatggcga caatgtagta gacagatcaa ctaaaatgaa
     4501 ctggtacgat ggccaaaccc taatgtacct attggagaat gttcacattg caggcgatta
     4561 caaccacatt gatgcgcgat ttcctgttca atatgtagtt cgccccatga cgactgaaca
     4621 ccatgattac cgaggctatg ccgggcgggt tgcaagtggt gtacttcgtg ttggtgatga
     4681 cattactgta cttccttctg gcttcgaatc gaaaatcaaa tcaatagata caagcaccgg
     4741 gatgcttgaa gaagcatttg caccaatgtc cgtttgttta acacttgaag atgaaattga
     4801 tatttcaaga ggagatatga ttgtccgccg gaacaacctg ccggaacaaa gtcaggacgt
     4861 ggaggctatg gtgtgttggt tcacagaaag acccttgcaa acaggaggta aatacgccat
     4921 caagcacaca agtaaagatg ttcgttgcat gataaaggac atccgataca aaatggatat
     4981 caatacgctg caccgaaatg aagaggataa aaacattaaa atgaatgata ttgcccgcgt
     5041 ggtgttacgc actacagcgc ctttatttac agataaatac catcggaata ggtttaccgg
     5101 gagttttatc cttatcgatg aaggaacaaa tgaaacagtc ggagccggaa tgattattta
     5161 atgaatttta ttttttcata cttttaaggg atgcggaaat catcccttct tcttttgttt
     5221 ggatttcttt ctatccactc ttgtcgtaga gccgataggg atttggaccg tgatatcagt
     5281 gaaacaaagg atcaggtttt cggaacctat gtgtggcttg atgttttttc gcgtgttagt
     5341 gaagcgtgtt catttgagcc cggtctgcgt atgctcgaat gcggcaatgt aatgatcgac
     5401 accttgtcgt cacccatgag catactaata gattttggac caaccgattg caccggtaat
     5461 gatggccgaa caagaagcgg gaaaattttt gcaagtttat cttccggata caatgattca
     5521 gctactattg ttcaggttca attacaaaat tatcgattca acggacaaaa cattcagggg
     5581 caaatgacaa taaccaataa cggcagggac acacaatcac ggagaaaatt tgctgttagt
     5641 gttcaacaat caacgattca attttcagct tcaaatagaa cttcgatttg gtcatcatca
     5701 ctaaccttat tgctaaattc cggagagact accgttgcta tttcagatga ttcatttctt
     5761 gtttcaggca ctttttccgg aaaaagctcc aagggaaatt tgtatacggg aaatatttca
     5821 caacctattt cctatcaact gagttgtggt gaaatcgtaa gcggtagcat tgatgttttg
     5881 cctgaaaacc ttaataagcg agttgcccta tttggggatg ggaattgtga tgcgattatt
     5941 ttagtgaacg taaatggttt tgaagaagaa tttaatccgg aatcaataga ttaaacagca
     6001 attttaaccg gactcagttg ctttccatct gcaatattgt gaatcgattc catccattta
     6061 aaaacatcca aagactgcac aattgcagct tcaacggggt ctgaacatag aagagaaaaa
     6121 gcattaaaat atctgttcat tgttgaagct ctgtcgaaag gtgtttttcc atttagcttt
     6181 ctcaccattt ccaaaaaggt aatttccggt gtatttaaac aattacattg ggaaagtttt
     6241 cttgaataag ttgcagtata cgatcgaacg agcattgctt cgccctgatc cagtgctgaa
     6301 agaatgagta gaggatagac tttaaaaaac aagctgtctt tgcaagcatc tttccctgca
     6361 accaatctat tagccattgc attgaactcc ttggtacggc cggaaaaaaa acaggtcctg
     6421 agacattgaa atgccagatt gttctcaagg agatttgtag ttcccgattc atctttccaa
     6481 agcatttcaa ccgcctccgg ctccatacga catgcctgat ccaagttacc ggaattcata
     6541 tggtattcca tttcgagggc cttgaattca agtgtagaat tcacatcatg cttgctcaaa
     6601 cgcaatacca tgattgcttc ggaagcaatt gaagatttag aagcggcaat tgcacaacgc
     6661 aggtataaca aacccaattt tgtcatcatc gtttcggaaa aaacagaccg ttccggtatc
     6721 tggtattctt tgaagcgatc ccattcattc tgcaacaatt ccagcgcttt ttgaggtttc
     6781 ccaaacagta atg
//
LOCUS       DAOMYG010000136         6750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3839_length_6750_cov_12.170426, whole genome shotgun sequence.
ACCESSION   DAOMYG010000136 DAOMYG010000000
VERSION     DAOMYG010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3839_length_6750_cov_12.170426"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1896
                     /locus_tag="PK610_05775"
     CDS             121..1896
                     /locus_tag="PK610_05775"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002665658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRJ35474.1"
                     /translation="MERFYDLFFTYPGFTCAELGKQANDFSKINNEPITFTQANFWIF
                     FLLVYGIFTLVHKKQNLRTWFLLAVSCFFYFKTSGLFLILLVFSTLLDYNIGNAIFKS
                     NRQGKRKFLLFLSMFGNLFVLGYFKYAFFFTDSFNQLFHTNYEFFNHFSYWTNSFWGS
                     EVLCVDRLILPVGISFYTFQTMSYTIDIYRKELQPVRNIIDFGFFVSFFPQLVAGPIV
                     RAKDFIPQMYQPFSLTKNDFGWSLWQILRGLVKKIVIADYLGANFVDHAFQNPDNFPG
                     FVLVVAMWAYSMQIYGDFSGYTDIAIGVSRLLGFQLNTNFNSPYKAINVADFWKRWHI
                     SLSSFLKDYLYIPLGGNKTASWVSWLFLPFIIAFIAGLIEYYFDVAFTVAGIEFSFGF
                     WTLSFILMVLVLLLMLIDSVRKFVTTDINLLITMLLGGLWHGNNLKFIIWGGLNGLGL
                     VIYKYWKRISPYEKSNFFLVHFWKIFFTFNFITFTRVFFRASDMENANLYMNRIWNNF
                     GLFTDEGFNNSAWNLMMTTYSIVFLLMIAAYLIHWMPSAGKEKIMNGFIRLPSYAQVV
                     VIVLSSILIYQAVSADFQAFIYFAF"
     gene            2069..5758
                     /locus_tag="PK610_05780"
     CDS             2069..5758
                     /locus_tag="PK610_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ35475.1"
                     /translation="MKQFKLLVGTLLLSLGSFTAFAGGPPSAVCTDRNEYVADVKEVG
                     ATGNYSLIGGTGALSRAAQTYFYSGSGTLTTVRVHGAVPVSFFRTRLVAKLYNVDATN
                     RPTGIVATSYYEDFYWFESYQDFDFPGVGPAITGNFAVSVELYNPGSPSDRFDVAYTG
                     DGEGNGEDLASLAGSSTGFNWSSAMTSFSKDGDFYIEPVVRHDISASFTKNAVCLGVG
                     QSVGFTNTSALTLAPMFNLANPHFEWDFGDGSAVATTTNASHSYATPGFYTISLTTYF
                     QGYGGTSCSHVVTQQVSVGLAASATNTNALCYGNNGSLTASATGGKTPYMYSLDGVAY
                     QSSATFSRPAGTYSVFVRDALGCVASTSVTIGQPTQVVIGSVPSTNSSCGNTNGALLI
                     NAAGGTAPLQYSINGGTNFQSSNSFSSLGAGIYNIVVKDANNCSVSTSATVIDNAGPS
                     IAFQAQTNVACHGSSTGSIAVIGSGGTGTLTYSINGTTFQSNGVFNNLPAGSYGVIVK
                     DQAGCINGLTIDITEPGALAQFGLNVNQHVSCNGGNDGQLQVAVGVGGIGTYSYSING
                     VNFQSSPVFSNLSAGSYTITVKDVAGCSTTLSAVINQPSALNLTASAVGASCWNATNG
                     TITLSANGGTSPYYYTVGESFSTTNNVFEGLGAGTYTVQVTDANGCTATSSIAVTQPA
                     PIQASITTGASTCGGSDGTLGVIGSGGSGAPYTYSIDNITFGAAGTYSGLAAGNYFIV
                     VRDNAGCEVVFNTIITTTTGPSINTVSSTNVSCFGGNDGSISVTNVTGGTGTLYYSLD
                     GSAFQTSNTFTGVTGGHHIIVVQDQVGCSSSDDITITEPAEIIVGTSINDHVSCFGYN
                     DGSVSVNAAGGIGQIVYSLNGGFFQSANTFNSLTAGLYQVVAKDAAGCTSSANFIVFQ
                     PTQILVGAGYLNVTCNGAQNGEIFVNSSGGTGTHEYSLNNVTYTSNSSFTGLNGGNYT
                     VFVQDDNGCVASIPVTLAEPAAIVLSSMVSNVSCAGGSNGVVNLSVSGGTPVYTFAWN
                     NGSTNEDIFNLVAGNYSVIVTDDNGCIKTATYSVTAPTNPIIINGVITNATGATNANG
                     SVDVTITGGTPPYSYSWSNGAVVEDLANVLPGVYSIQVTDAAGCQTSVGFTVNFTVGV
                     DEAVAMEAANIYPNPANYVVNVDAGDQNIRGIRMVNMVGQIVYSAQPNATKTTIDLSS
                     IASGVYFVQIEGDNGMVTKKIEVVK"
     gene            complement(5841..6509)
                     /locus_tag="PK610_05785"
     CDS             complement(5841..6509)
                     /locus_tag="PK610_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35476.1"
                     /translation="MIWKSFCFLGVMVCLNANASKHAPISVNKKVAAVVADSMQFVFG
                     SNKNVPEKFRLPFYYALQFYPELQSLHISVKEKKISTTMAARPSSGSVVFRFRKKRHY
                     IVFVNSDTKGKAPHYDEFSFNAQIGVFGHELAHFLKYIKQGVFSLMGDALKYRKDSFK
                     SHYECYTDSITIARGLGWQCYDFSTQLQNNQRIDSSYKSRKEELYYTPRKIFALMSDL
                     GYDM"
     gene            6542..>6750
                     /locus_tag="PK610_05790"
     CDS             6542..>6750
                     /locus_tag="PK610_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28 family protein"
                     /protein_id="HRJ35477.1"
                     /translation="MKTRILFSLLCLLTFSTSKAQSDSITVRRIYDIALTQGECYENL
                     RVLCKQVGHRLSGSAQAEKAVVWGK"
BASE COUNT         1870 a         1395 c         1347 g         2138 t
ORIGIN      
        1 cagggtccat tttacacttg aaggctacaa tttaaaaggt gatttgtttt ttgaagcctt
       61 tttaaaatac cttaatgaat ttgaataccg aagactgatt aaactgagta acgaaaaata
      121 atggaacggt tctacgatct gttttttacc taccccggat ttacctgtgc cgaacttggt
      181 aagcaggcga acgacttttc caagatcaat aatgaaccta tcacctttac tcaagccaac
      241 ttctggattt tctttttact tgtttacgga attttcacac tcgttcataa aaagcaaaat
      301 cttagaacct ggtttctgct tgcggtaagt tgctttttct attttaaaac aagcggacta
      361 tttttaattc ttcttgtttt ctccaccctt ctggattaca acattggaaa tgcgatattc
      421 aaatccaatc ggcagggtaa acgaaagttt cttttattcc tttcgatgtt tgggaatcta
      481 tttgtattgg gttattttaa atacgcattc ttttttacag attcctttaa tcaactcttt
      541 cataccaatt acgaattctt taatcacttt tcctactgga caaattcatt ttggggatct
      601 gaagtattgt gtgtagaccg attgattctt ccggtaggta tttccttcta cacttttcaa
      661 acgatgagtt acaccatcga catttacaga aaagaacttc aaccggtacg gaatattatt
      721 gattttggat tttttgtcag cttctttcct caattggttg ccggtccgat cgtacgcgcg
      781 aaggatttca taccacagat gtatcagcca ttttctctta caaaaaacga ttttggctgg
      841 tcgctatggc aaatcctgcg aggattggtc aagaagattg taattgcaga ttaccttgga
      901 gcaaactttg tggatcatgc ctttcagaat ccggataatt ttccgggatt tgtattggtt
      961 gttgccatgt gggcttattc gatgcagatc tacggcgatt ttagcggtta taccgatatt
     1021 gccattggtg tttcccgact acttggtttc cagctgaaca ccaatttcaa ttcgccttat
     1081 aaagctataa acgttgcaga cttttggaaa cgctggcata tttccttgtc atcgttttta
     1141 aaagactacc tctacattcc tttaggcggc aataaaactg caagctgggt ttcatggcta
     1201 ttcctaccat ttattattgc atttattgca ggattaattg aatactattt tgatgttgcc
     1261 ttcactgttg cagggattga attcagtttt ggattctgga cactctcctt tattcttatg
     1321 gtgctggtat tactgcttat gctgatagat tctgtacgga aattcgtaac aacagatatc
     1381 aatctcctta ttacaatgct ccttggtgga ttgtggcatg gtaacaattt aaaatttatt
     1441 atctggggag gattaaacgg actgggatta gtcatctaca aatactggaa acgaatttct
     1501 ccatacgaga aatcgaattt cttccttgtt catttctgga aaatattctt caccttcaat
     1561 ttcattacgt ttacaagggt atttttccga gcttcagata tggagaatgc caacctatat
     1621 atgaatcgga tctggaacaa ttttggatta tttacagatg aaggttttaa taacagtgcg
     1681 tggaatctga tgatgaccac ctattcaata gtattccttt taatgattgc tgcataccta
     1741 atacattgga tgccttctgc cggaaaagaa aaaataatga acggctttat ccgcttacca
     1801 tcttacgcac aggtagtcgt tattgtacta tcaagtatac ttatctatca ggctgtctct
     1861 gcagatttcc aggcattcat ctatttcgca ttttaggatt tcatcttttg cggtcataaa
     1921 tattcctgac ccgcatcagt ttcaggtacg atcgtgacca ttgtcatgct tttttcatcc
     1981 gtatgtctga ctacgctcat cctttcaagg cttaacacag aactaatttt ggactgtaaa
     2041 cattaagtct aactaaaccc tgaaaacaat gaaacaattt aaactccttg tcggaacgtt
     2101 attgttatcg ttaggaagct ttacagcctt cgccggagga ccaccctcgg cagtttgtac
     2161 tgatcgtaac gaatacgttg ccgacgttaa agaagtagga gcaacaggca actattcttt
     2221 aattggcggg acgggagctc tctctcgtgc tgcgcaaacg tatttttaca gcggatcagg
     2281 tacacttact acggttcgtg tacatggtgc ggttccggta tcatttttcc gtacccgtct
     2341 tgtagcaaag ctatataatg ttgatgcaac caatcgccca accggtattg ttgcaactag
     2401 ttattatgaa gatttctatt ggttcgaatc gtaccaggat ttcgattttc ccggtgttgg
     2461 tcctgctata acaggaaact ttgctgtttc agtagaactt tacaatccag gtagcccttc
     2521 cgatcgtttc gatgtagctt atacaggaga cggtgaagga aacggtgaag atcttgcttc
     2581 acttgcaggt tcttcaaccg gctttaactg gagcagtgcg atgacaagct tcagcaaaga
     2641 cggtgacttc tacattgaac ctgttgttcg tcacgacatt tctgcttctt ttaccaagaa
     2701 tgcagtttgt cttggtgtag gtcagtcagt aggtttcaca aatacttctg cactaacact
     2761 cgcaccaatg tttaaccttg ccaatccaca ttttgagtgg gattttggtg atggatctgc
     2821 agttgccaca actaccaatg catcgcattc gtatgcaact cccggtttct acactataag
     2881 tcttactact tacttccagg gttatggcgg aacatcttgc tcgcatgtcg taacgcaaca
     2941 agtttctgtt gggttagctg catctgcaac aaacacaaat gctttgtgtt atggcaacaa
     3001 tggtagcctt acagcatctg caaccggtgg aaaaacacct tatatgtata gtctcgacgg
     3061 agtagcttac caatcttctg ctacattttc tcgtcctgcc ggaacatata gtgtttttgt
     3121 aagagatgca cttgggtgtg ttgccagtac atctgtaact atcggacaac ctactcaggt
     3181 tgttatcgga tcagttcctt caaccaactc ttcttgtggt aacacaaatg gtgcacttct
     3241 tattaatgct gcaggtggaa cagctccact gcaatacagc attaatggag gaacaaattt
     3301 ccaaagtagt aactcgttct ccagtcttgg tgccggtatt tacaacatcg ttgtaaaaga
     3361 cgctaacaac tgttctgttt ctacttcagc aactgtaatt gacaatgcag gtccaagtat
     3421 cgcattccag gcgcaaacta atgttgcgtg ccatggttca agcacaggaa gcattgcagt
     3481 tatcggatca ggcggaacag gcactcttac ttatagtatc aatggaacaa ctttccagtc
     3541 gaacggtgta ttcaacaacc ttcctgccgg aagttacggt gtgatcgtta aagaccaggc
     3601 aggttgtatc aatggtctca ctattgatat tacagagccc ggggcacttg ctcaattcgg
     3661 acttaacgtg aatcagcatg tatcatgtaa cggaggaaat gatggtcaat tgcaagttgc
     3721 agttggagtt ggaggaattg gaacttattc atacagcatc aacggtgtta atttccagtc
     3781 atcacctgtt ttctctaatc tttctgcagg atcttatact atcaccgtaa aagatgtagc
     3841 aggatgttct actacacttt ctgctgtaat caatcagcct tctgctctta accttactgc
     3901 cagtgcagtt ggagcatctt gctggaatgc aacaaacggt actattactc tttcagcaaa
     3961 tggtggaact tctccatact actacacagt aggtgaaagt ttttcaacaa ccaacaatgt
     4021 attcgaagga cttggtgcag gtacttatac tgtacaggta acagatgcca acggttgtac
     4081 tgctacttca tctattgccg ttacacaacc tgctcctatt caggcttcaa ttactacagg
     4141 tgcgtctacc tgcggtggtt ctgatggaac acttggcgta attggatcag gtggatcagg
     4201 tgcaccttat acttattcta tcgacaatat tacttttggc gctgccggta cctattcagg
     4261 tcttgcagca ggtaattact ttattgttgt tagagacaat gcgggatgcg aagtagtatt
     4321 caataccata atcactacta ctacaggtcc tagcatcaat actgtttctt ctacaaacgt
     4381 ttcttgcttc ggtggaaacg acggcagtat cagcgttaca aacgtaacag gtggaacagg
     4441 aactctgtat tacagtcttg acggttctgc tttccaaacc agcaacacgt ttaccggtgt
     4501 aactggtggt catcacatca tagttgttca agatcaggtt ggttgcagca gcagcgatga
     4561 cattaccatt actgaacctg cagaaatcat tgttggaact tctatcaatg atcatgtaag
     4621 ttgttttggc tacaacgacg gaagcgtttc tgtaaatgca gcaggtggaa ttggtcagat
     4681 tgtttacagt cttaacggag gattcttcca atctgcaaat acgtttaatt cattgactgc
     4741 aggtctttac caggttgttg caaaagatgc agcaggttgt acctcatctg cgaatttcat
     4801 tgtattccaa cctacacaaa ttcttgtagg tgcaggatat ctgaacgtaa catgcaacgg
     4861 tgcccaaaat ggtgagattt ttgtaaactc cagcggagga acaggtactc atgaatattc
     4921 actgaacaac gttacctaca catctaatag ttcttttact ggtctaaatg gtggtaacta
     4981 cactgttttt gttcaagacg acaacggttg tgttgcatca attcctgtaa cacttgcaga
     5041 acctgcagcg attgttctct cttcaatggt gagcaatgtt tcttgtgctg gcggaagcaa
     5101 cggtgtggtt aacctttctg taagcggtgg aactccggtt tatacgtttg cttggaacaa
     5161 cggttcaacc aacgaggaca tctttaacct tgttgccgga aactattcag taattgtcac
     5221 tgatgataat ggttgtatta aaacagcaac ttattctgtt actgctccta ctaatccgat
     5281 catcatcaat ggtgtaatta ctaacgcaac aggcgcaaca aatgcaaatg gttcagttga
     5341 tgtaactata actggtggta cacctcctta ttcttacagc tggtcgaatg gcgcagttgt
     5401 tgaagatttg gcaaatgttt taccgggtgt atacagcatt caggtaaccg atgcagcagg
     5461 ttgtcagaca agtgttgggt ttacagtaaa ctttacagtt ggtgttgacg aagcagttgc
     5521 tatggaagca gcaaacatct atcctaaccc tgcaaactac gttgtaaatg tagatgcggg
     5581 agaccagaac atccgcggaa tccgtatggt gaacatggta ggacaaattg tttacagtgc
     5641 acaaccaaat gctacaaaaa caacaatcga tctttcttct attgcatccg gtgtttactt
     5701 cgttcaaatc gaaggcgaca atggaatggt aaccaagaaa attgaagttg taaaataatt
     5761 tgaaaatacc ctgatcacaa gaggggagcc ttagggctcc ctttttgttt ttatcaaaac
     5821 cggatgttgc atttcaaaag ctacatatcg taccccaaat cactcattaa ggcaaaaatt
     5881 tttcttggtg tataataaag ttcctctttt cttgatttgt aagaagagtc aattcgttgg
     5941 ttgttttgaa gctgggttga aaaatcataa cactgccatc ctaaacctct ggcgatggta
     6001 atactatcgg tataacattc atagtgtgat ttaaaactgt ccttccggta tttaagcgca
     6061 tctcccatta atgaaaaaac tccttgttta atgtatttca gaaagtgagc aagttcatga
     6121 ccgaaaacac caatctgagc attaaaagaa aattcatcgt aatgtggtgc tttccctttt
     6181 gtatctgaat tcacaaatac aatataatga cgcttttttc taaaacgaaa cacaacagaa
     6241 cccgatgatg gccgcgccgc cattgttgta cttatttttt tctctttcac tgaaatgtgc
     6301 aaggattgca actcaggata aaactgcaag gcataataaa atggtaatct gaatttctcg
     6361 ggaacgtttt tgttggatcc aaaaacaaat tgcatggaat cggcaacaac agcagccact
     6421 tttttgttta cgcttatggg agcgtgtttg gatgcatttg cgttcagaca caccattaca
     6481 cccaaaaaac aaaaactctt ccaaatcata ttctttgtaa atttagccag accaaccgga
     6541 aatgaaaaca agaattctct tctccctact ttgtttgctg accttcagca cttccaaagc
     6601 ccaatccgac tccattaccg ttcgtcgcat ttatgatatt gctctaaccc agggcgaatg
     6661 ctacgaaaac ctaagggtgt tatgtaaaca agtcggacat cgtttgagcg gttcagcaca
     6721 agcggaaaaa gcggttgtct ggggaaaaca
//
LOCUS       DAOMYG010000137         6682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3897_length_6682_cov_9.021126, whole genome shotgun sequence.
ACCESSION   DAOMYG010000137 DAOMYG010000000
VERSION     DAOMYG010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3897_length_6682_cov_9.021126"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PK610_05795"
     CDS             complement(<1..316)
                     /locus_tag="PK610_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase group 1"
                     /protein_id="HRJ35478.1"
                     /translation="MPGKHISVAVTNDLLTDQRVHKVCTYLCEKGNTVTLIGRKRKNS
                     TELPARAYKTVRMRLLLEKGAFFYAFFNLRLFFRLLFSKSDIIVSNDLDTLLACRMAS
                     RLK"
     gene            480..1496
                     /gene="murB"
                     /locus_tag="PK610_05800"
     CDS             480..1496
                     /gene="murB"
                     /locus_tag="PK610_05800"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009087948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRJ35479.1"
                     /translation="MNLLENISVKSYNTFGIDVSTRFLTEAHSVDDLKEIIEDRRFAA
                     VPMMILGGGSNVLFTENYFGLIVLNRLHGISLEREDENHYYVKAGAGENWHAFVMWCV
                     ERNYAGLENLSLIPGCVGASPMQNIGAYGVEMKDHFHHLEALNTETLETERFEFGDCR
                     FGYRESVFKRELKGQYIITSVTFRLDKQPKFRTDYGAIAQELEAMGVKDLSIRAVSDA
                     VIRIRRSKLPDPQVLGNAGSFFKNPEITEEQKIQLLAMHPDAVVYPLENGNYKAAAGW
                     LIEKAGWKGKRIANYGVHEKQALVLVNYGGAQGKEIFDLSSQILDDVKSRFGIQLERE
                     VNIV"
     gene            1682..2641
                     /locus_tag="PK610_05805"
                     /pseudo
     CDS             1682..2641
                     /locus_tag="PK610_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
     gene            complement(2936..3334)
                     /locus_tag="PK610_05810"
     CDS             complement(2936..3334)
                     /locus_tag="PK610_05810"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ35480.1"
                     /translation="MNLILLRGLPGAGKTTLAKLLSEEKWPCYSVDDFFTDEKTGDYS
                     FDHKQNHLAYKQCEENTRIALQSGIEKVFVHNTFTIDWELEPYFKMAAEFGYALHIVT
                     VENYHGNTNVHQIPNEQLQKMAEKYKVRLM"
     gene            3536..4342
                     /locus_tag="PK610_05815"
     CDS             3536..4342
                     /locus_tag="PK610_05815"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ35481.1"
                     /translation="MKIQGKTIVVTGGGNGIGRELSLQLLKGGARVIAVDLFADALAE
                     TKKLAGDRAGNFSSHVLNIADRNAVEQFRDTVLQSGPVDGLINNAGIIQPFIKVDELD
                     YTAIERVVNVNFYGTLYLTKSFLSHFKSRPAAHIVNISSMGGFLPVPGQSVYGASKAA
                     VKLLTEGLHLELKNTNVKVTIVFPGAIATNIAANSGVNIEMKPGSEKPQKFKALPVQD
                     AAAKIIKAMEQDAFRVTVGSDARFMDIIYRISPRRAAYFIYNKMKSMLGE"
     gene            complement(4354..5418)
                     /locus_tag="PK610_05820"
     CDS             complement(4354..5418)
                     /locus_tag="PK610_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRJ35482.1"
                     /translation="MEDFLAKEFYGNTAEQWLITLGIIFGSVILAKIAYWIIGKTIKQ
                     ITSRTKSKLDDILVDQFEEPGVMAIIIFGSWYGVGLLSTTEEVKQFLDNTFILATAIN
                     ITWFVSRLVNSILEEYLTPYVQKSDNTLDDALLPVLRRGLRILIWSIGIIMGLNNAGF
                     DVAALIAGLGIGGLAIALASQDTVKNVIGGLMIILDKPFKIGDRIMIDSFDGTVEDIG
                     VRSTRIRTMDGRQVIIPNMIFSDKPITNITREPARRIMVTLGLVYQTTPEQMEIARQT
                     LKEIAGSHELIEHENTRVFFGNFGPHSLDITLVYFIIAGNDYFEVQNTINTSILKEFN
                     ALGLQFAYPTQTVYKIDQKA"
     gene            5534..6151
                     /locus_tag="PK610_05825"
     CDS             5534..6151
                     /locus_tag="PK610_05825"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRJ35483.1"
                     /translation="MNIAIIGTGNVGGALATRWAEKGHRIFLGVRNSNEFKGMHLLSN
                     SNTTAHSIHDAVAQAEVVLIATPAPLAVEVAKSLGDSTGKVIIDSMNIVLGRGPQGYT
                     TTAEAILDHTGSRDVVKCFNTTGFNNMLNPTYGDQVLDMFVAGDSEKGKSIAVQLALD
                     AGFAACYSVGGNDRFALMEQFAFFWINLAMMQGQGRDIGFKLLKR"
     gene            complement(6169..>6682)
                     /locus_tag="PK610_05830"
     CDS             complement(6169..>6682)
                     /locus_tag="PK610_05830"
                     /inference="COORDINATES: protein motif:HMM:NF017747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HRJ35484.1"
                     /translation="LYIFPHTSNYSDRNFDFRFSTAIIEQAESEFTSLPGVERILMLL
                     EGKLIIRHAAHYEKELFPFDSDQFSGSWNTSSVGTGRDLNLMLCNGASGSIRAIEIPT
                     GERWELQSEPSWNSHDLFWLYKGKLDHPDGKEFLSEGDLLIYSPEENEFPIGNAKEDC
                     ILIHAVVKIP"
BASE COUNT         1868 a         1273 c         1618 g         1923 t
ORIGIN      
        1 gttttaaccg cgaagccatt ctgcaggcca gtaaggtgtc gagatcgttc gaaacaatta
       61 tatcggattt ggaaaacaga agtcggaaaa aaagacgcag attaaaaaaa gcataaaaga
      121 atgcaccttt ttcaagcagc aagcgcatac gtaccgtctt gtaggctctt gctggaagtt
      181 cagtgctgtt ttttcgcttt cttccgatga gcgtaacggt atttcctttt tcgcacaggt
      241 aagtacacac cttatgcacc cgttgatcgg tgagcagatc gttggtgact gcaactgaaa
      301 tgtgtttgcc tggcattcga cgaaaatacg gtaaaaagac ttttgtaaga ttcgatttcc
      361 tgtaactgtg ggcgaataag gtctgaaatc acgatttggt cttcagaaat cactttccgc
      421 tgttgaatac tatttgccgg gagggtatca gcagactcca tttccgatag atttgctgta
      481 tgaatctgct ggaaaacatc tccgtaaaga gctacaatac atttggtatt gatgtcagca
      541 cccgttttct caccgaggcc cattcggtgg atgatctcaa ggaaataatc gaagaccgtc
      601 gcttcgctgc tgttcccatg atgatacttg gtggaggaag caatgtgttg tttactgaga
      661 attatttcgg tttaattgtt ctgaaccgac ttcatggtat ttcgctggag agggaagatg
      721 aaaatcatta ttacgtaaaa gcaggagccg gagaaaactg gcatgcgttt gtgatgtggt
      781 gtgtggaaag aaattatgca ggacttgaaa atttatcgct gattccggga tgtgttggag
      841 cttctccaat gcagaatatc ggagcatacg gtgtggaaat gaaagatcat tttcatcatc
      901 tcgaagcatt gaataccgaa acactggaaa ccgagcgctt cgagtttggt gattgtcgtt
      961 ttggttaccg cgaaagtgtt ttcaaacggg aactcaaagg acaatacatc atcacctcgg
     1021 taacttttcg tttggataag caacccaagt tccgaaccga ttatggtgca attgcgcagg
     1081 agttggaagc gatgggtgtt aaggatcttt ccattcgtgc cgtttcggat gctgtaattc
     1141 gtattcgccg aagtaaatta cctgatccgc aggttttagg caatgccgga agttttttca
     1201 agaacccgga aattacagaa gaacaaaaaa tacaattact cgcaatgcat cctgatgcgg
     1261 ttgtttatcc gctcgagaat ggaaactaca aagcagcggc cggatggctt attgaaaaag
     1321 ctggatggaa aggaaagcga atagccaatt acggcgttca tgaaaaacag gcgctggtat
     1381 tggtcaatta cggcggagcg cagggaaaag aaattttcga tctctcttcg caaattctgg
     1441 atgatgtgaa atcccggttc ggtattcagc tggagcggga agtcaatatc gtttagatca
     1501 gcatttgcct gcgaaaattc acacttcatt caggacttga ttaccgtttt gagcaggcaa
     1561 tatttttgtc ttgctgttct gatttggcgt taagtccatg taaataagcg cattaacagc
     1621 tttatttatc ggtgttgcta attctcattt ttcgtaaatt gcgcccctaa tccagaatgg
     1681 aatggccaat taccttgatt ttgaaaagcc cattgaagat ctgattcacc agatcgaaga
     1741 cgcgaagagc attgcagaga aaacgaatgt tgatatgggc aaggcaatca aagaccttga
     1801 aaagaaattg cagcaaacac gtatcgatat ttttgcgaac ctgagtccat ggcaacgtgt
     1861 tcaggcatcg cgtcatcctg atcgtcctta ttcgcttgct tacatcaact acatcaccga
     1921 taatactttt caggaacttc atggtgatcg taatgtgcgc gacgataaag cgattgttgg
     1981 cggattcggt aagattggtg gtaacggaca aaccgttatg tttatcggtc atcagaaggg
     2041 aattaatttg aagatgcgtc agtaccgtaa tttcggtatg gccaatcctg aaggataccg
     2101 taaagcttta cgtttgatga agcttgcaga aaaatttggt aaacccattg taacattcat
     2161 cgatacaccc ggagcatatc ccggattgga agcagaagaa cgtggtcagg gtgaagctat
     2221 tgcccgcaat atttttgaga tgatgcgact gcgtgtacct gtgatctgtg tgatccttgg
     2281 agaaggcgca tcgggtggtg cattgggaat tggtgtaggc gatcgtgtgc ttatgctgga
     2341 gaatacctgg tattcggtga tctctcccga atcctgttca tccatattat tccgcagctg
     2401 ggaaaagaag gagattgcag caacagcact caaacttact ccgcaggata tgctgaagaa
     2461 caagttgatc gatggcatca ttaaagaacc tgttggtggg gctcatactg atccgctcgg
     2521 cgcacatgaa attgtgaagc aggaactgat gcgtcagctt gcagagttga aaaaaatcaa
     2581 agccgataaa ctggtaagcg aacgtatcga taaattctgt ggcatgggag tctttatcga
     2641 tgaagatgca cctgccggaa aacagaaagc ggaaaagaaa accgccaaaa aaaacagccc
     2701 gaccaaaaag acaacacctg caaaaaaagt atccaaaccc gcagcgaaaa aagcagtagc
     2761 caaaaaacct gctgtaaaaa aatcagctcc caaaaagatt actgcaaaga aaacagcagt
     2821 gaaggggaag aagtgatttt ggtgaagggt gaacagtgaa aggtgagcag tgatctgtga
     2881 acagcgctga gtgtttttag aagggttgtt caattagagg tgaataattg gtgatttaca
     2941 tcaaacgcac tttgtatttc tctgccattt tttgcaattg ttcattggga atctgatgaa
     3001 catttgtgtt gccgtggtaa ttttctacag tgacaatatg caaggcatag ccaaattcag
     3061 cagccatttt gaaatagggt tcgagttccc aatcgatggt aaaggtgttg tgaacaaata
     3121 ctttttcgat gccggattgt aacgcgattc gtgtattttc ttcgcattgt ttgtaggcga
     3181 ggtgattttg tttgtgatcg aaggaataat ctcctgtttt ttcatccgta aaaaaatcat
     3241 ccaccgaata gcagggccat ttctcttcgc tgagaagttt cgccaatgtt gttttaccgg
     3301 caccggggag tccgcgaagg aggatgaggt tcatggggtg tttggttaat gtgagaatga
     3361 gtgaatcccg acgctccttt tcgtcggtcg ggatgattct cccttctgtc gaatcatgtg
     3421 gaaatgtttt taagagacaa aaataggtgt ttttttaaat agaattggct ttgctgacta
     3481 ttgtcatgtt tttgaattga agtttggatt agtattgtta aatattgaaa atcctatgaa
     3541 aattcaagga aaaaccattg tagttaccgg tggaggtaat ggaattggaa gggagctttc
     3601 gcttcagtta ctaaaaggcg gtgctagggt tattgctgtt gatctttttg ctgatgctct
     3661 tgctgaaaca aaaaaactgg caggagatcg tgccggtaat ttttcttcgc atgtgctgaa
     3721 tattgcagac aggaatgctg ttgaacagtt cagagatact gttttgcagt caggaccagt
     3781 ggatggtctg atcaataacg cgggcattat tcagcctttt attaaagtgg atgaactgga
     3841 ttatactgct attgagcggg tagtgaatgt gaatttttac ggaacacttt acctcaccaa
     3901 gtccttttta tctcatttta aatcacgacc tgcagcgcat atcgtgaata tttcaagtat
     3961 gggtggattt cttcctgtgc ccggacaaag tgtatatggt gcttcaaaag cagcagtgaa
     4021 attgcttaca gaaggattgc atcttgaatt gaaaaataca aatgtaaaag tgaccatcgt
     4081 tttccccgga gcaattgcaa ccaatattgc agcgaattcc ggtgtgaata ttgaaatgaa
     4141 accgggatcg gaaaagccac aaaaattcaa ggcactaccg gttcaggatg ctgctgcaaa
     4201 aattattaag gcgatggaac aggatgcctt tcgtgttacc gttggttctg atgcacggtt
     4261 tatggatatc atctaccgca tcagtccgcg acgtgctgcg tatttcatct acaataaaat
     4321 gaaatcaatg ttgggcgaat aataatttat tcctcaggcc ttctggtcga ttttgtacac
     4381 tgtttgcgtg gggtaggcaa actgcaatcc tagtgcattg aattctttta gaatcgaagt
     4441 attgatggtg ttttgcactt cgaaatagtc gttacccgca atgatgaagt ataccagtgt
     4501 gatatcgagc gaatgcggtc caaaattccc aaagaatacg cgcgtgtttt cgtgttcgat
     4561 caattcgtgg ctgcctgcaa tttctttcag tgtttggcga gcaatttcca tttgttcggg
     4621 agtggtttgg taaacaagtc ctaacgtaac cataattctt cttgcgggtt ctcgggtgat
     4681 gttggtgatg ggtttatccg agaaaatcat attcggaata atcacctgcc tgccatccat
     4741 tgtgcggatt cgggtacttc tcacaccgat gtcttcaact gttccgtcaa aggaatcgat
     4801 catgatgcgg tctccgatct taaatggttt gtcgagaatg atcatcaatc cgcctatgac
     4861 attctttacg gtgtcttgtg atgcaagtgc aatggcaaga cctccgattc cgagtcctgc
     4921 aataagcgcg gctacatcaa atcccgcatt gttgagtccc atgataatac cgatcgacca
     4981 gatcaatatc ctcaatccac ggcgaagaac ggggaggaga gcatcatcga gggtgttgtc
     5041 gcttttctga acgtagggag taaggtattc ttccagaatg gaattcacca gtcgcgatac
     5101 aaaccaggtg atgttgatgg ctgttgcaag gatgaaggta ttgtcgagaa attgtttcac
     5161 ttcttccgta gtagagagca atcctacacc ataccatgag ccaaaaatga taattgccat
     5221 tacaccgggt tcttcgaatt ggtcgaccag aatatcgtcg agtttcgatt tggtgcgaga
     5281 ggtgatctgt ttgatcgttt ttccgattat ccaatacgct attttggcca ggataactga
     5341 tccaaaaatt attccgagtg tgatcagcca ttgttcggca gtattgccgt aaaattcttt
     5401 ggcaaggaaa tcttccatag ttattgggtt taatgcctgc gaatatagtc agaaaacaat
     5461 aattgtgttt ggattgttga tagcatcggt ttcagtgtga aaaagactta tttttagttc
     5521 aaaattaccg gaaatgaaca tcgcaatcat cggaacagga aatgtaggcg gagcactggc
     5581 tacccgctgg gcagaaaaag gacatcgtat ttttctcggt gtacgcaata gcaatgagtt
     5641 taagggcatg catctgctct cgaattccaa taccactgct catagcattc atgatgctgt
     5701 agcgcaggct gaggtggtgc tgattgccac tcccgcgcca ttagctgtgg aggttgcaaa
     5761 aagtttggga gacagcaccg gaaaggtgat catcgacagc atgaatatcg ttttgggaag
     5821 gggtcctcag ggatatacta caactgcgga agcaattctt gatcatacag ggagtcgcga
     5881 tgtagtgaaa tgtttcaata caaccggttt caataatatg cttaatccca cttacggcga
     5941 tcaggttctt gatatgtttg ttgcgggcga cagcgaaaaa gggaagtcca ttgctgttca
     6001 attggcgctg gatgcaggtt ttgcagcttg ttattcggta ggtggcaacg atcgttttgc
     6061 gctgatggaa caattcgctt ttttctggat caatctagct atgatgcagg ggcagggcag
     6121 ggatattggg tttaaattgt tgaagcgata aaacaagcgc aatcttgctt aggggatttt
     6181 caccaccgca tgaatgagta tgcaatcttc cttcgcatta ccaatgggga attcgttttc
     6241 ttcgggagaa taaatcagta aatcgccttc gctgagaaat tcttttccat caggatgatc
     6301 taattttccc ttgtacaacc agaacaaatc gtgcgaattc catgagggtt cactctgcaa
     6361 ttcccagcgt tctcctgttg gaatttctat ggctcttatg gatccgcttg ctccgttgca
     6421 taacatgaga ttaagatctc tgcctgtacc aacggaactt gtgttccacg atccggagaa
     6481 ttgatcactg tcgaaaggga acaattcctt ttcgtaatga gctgcgtgac gaatgattag
     6541 ttttccctcc agcaacatca gaatgcgctc tactccgggg agtgaagtga attcgctttc
     6601 ggcttgttca atgattgcag tgctgaaacg aaaatcaaaa ttgcggtcgg aataattact
     6661 tgtatgcgga aaaatatata ac
//
LOCUS       DAOMYG010000138         6667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3907_length_6667_cov_7.667423, whole genome shotgun sequence.
ACCESSION   DAOMYG010000138 DAOMYG010000000
VERSION     DAOMYG010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3907_length_6667_cov_7.667423"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PK610_05835"
     CDS             complement(<1..287)
                     /locus_tag="PK610_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35485.1"
                     /translation="MHPEIQFYHSSLENSEQKICEFLEHTINSVLKKQESKIWHAHPV
                     WFIDENPIVGYSKQKKGIRLMFWSGADFEEAAEFADGASVEASAAASVMAPS"
     gene            complement(372..1655)
                     /gene="murF"
                     /locus_tag="PK610_05840"
     CDS             complement(372..1655)
                     /gene="murF"
                     /locus_tag="PK610_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013549577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRJ35486.1"
                     /translation="MIEQLYSHYCKYPQVVTDTRKLIPGSLFFALKGPNFDANQFAEQ
                     ALEQGCAMAVVDDPNVVKDNRFFLVGDVLTALQELAKYHRQQLKIPVLALTGSNGKTT
                     TKELMHAVLSEQFNTLATSGNLNNHIGVPLTLLSIRPEHEFAIIEMGANHQKEIAALS
                     RIALPEFGLITNIGKAHIEGFGGVEGIKKGKGELYDFLRTGNFLAFVNADSDVLMEMS
                     EGMNRKLYGHGEGNYMRGLNKGANPFLEMEIAENGKSISVKTQIVGKYNFDNALAAAA
                     VGKYFGLSLQQIKAGLEKYVPENNRSQILQKGNNTLILDAYNANPSSMRVALENFAEM
                     KASNKFFIIGDMLELGDASPEEHLSMIELSRQLGLKGAFVGPLFGSQIKDSSLLHFLD
                     TAAAKKFIAEHPFQQYHILIKGSRGMRLEGLLEVI"
     gene            complement(1749..2771)
                     /locus_tag="PK610_05845"
     CDS             complement(1749..2771)
                     /locus_tag="PK610_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRJ35487.1"
                     /translation="MLGSKKTGLLARVLTVFIFLTASPLLKAQGGETYEGKEIVDTLR
                     MNNSTIILFADKTWMYVSSTNFNGVMNDYLHKVVSTDTSLNFKTTWYNEIPFTYENDI
                     SRLHDTIWICTVDSTYSKFSMPTNGQVLSKFKYRGSRFHHGVDIDLNVGDTVRAAFSG
                     RVRYAQYNKGGFGNLVIIRHHNGLETYYAHLSKITVQVDQEVHSGELIGLGGNTGRSY
                     GPHLHFECRFYDNAFDPYEIIDFDNKRLQNENLFIHPGLFDYRAVSRKNTQPKGHSHE
                     PVMAGTGQTVKNSNSAGAKYYTVRSGDNLSVIARNHGTTVDKLCQLNGMNRNDVLRVG
                     QKLKVK"
     gene            complement(2896..3942)
                     /gene="meaB"
                     /locus_tag="PK610_05850"
     CDS             complement(2896..3942)
                     /gene="meaB"
                     /locus_tag="PK610_05850"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRJ35488.1"
                     /translation="MSGHDDSSYINPSFRRKKAPSLVDTETLFTDLTKGDRAALSRAI
                     TLIESEREDLQEAASNLLQRCLPLSGKSFRIGITGLPGAGKSTFIEAFGKFLTNKGFK
                     VAVLAIDPTSAVHKGSILGDKTRMESLSTDPNAFIRPSPSGAALGGVARKTRESMYLC
                     EAAGFDIVLVETVGVGQSETEVHSMVDFFLVLTIAGGGDELQGIKRGIMELTDLLLIN
                     KDDGTNTDATRRSKAEYAAALHLFPAPESGWTPRVETCSALENKGIDKAWSIIDEFRT
                     LTLSNGWIERSRKEQNLFWLRTSIFNQLEYSFFHHPDIKSQLPDLEQKVADLSISPFE
                     AAKKLLEIYRQSPK"
     gene            complement(3988..4548)
                     /locus_tag="PK610_05855"
     CDS             complement(3988..4548)
                     /locus_tag="PK610_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35489.1"
                     /translation="MRKTLLFIGALFILCSSGNDLQVRVCYGMALVNSANSQIASFAV
                     LTLQDNRIIRTQHISVDMFILQATGNATSLANPDAKDFFAENGISDCYPDYEMHSDKY
                     IGYWCPLITDLWKIRYKRDPKNAHLRQDEGWAKGYYAPSYEQMKYLEKEYGVKNMNDF
                     FIGEKLFKLLKDVTDTTWQNAYKSLP"
     gene            complement(4795..5367)
                     /locus_tag="PK610_05860"
     CDS             complement(4795..5367)
                     /locus_tag="PK610_05860"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRJ35490.1"
                     /translation="MVKEELRIEYLERMRSLTTGQVEDSSQSLASLFFREFPLYEECV
                     SIFLSITRNNEINTFYFINQLQREFPGVQLAVPVSDFSSGTMTHRRYHLHTTLKINKL
                     GIPEPIDGEVVEASCFDLVLVPLLCFDKNGSRVGYGKGFYDRFLSTCRPQTQKIGLSL
                     FPPVELIEDTNAHDIRLDAVITPGEIFRFN"
     gene            complement(5371..6147)
                     /locus_tag="PK610_05865"
     CDS             complement(5371..6147)
                     /locus_tag="PK610_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HRJ35491.1"
                     /translation="MNEFLLIGLTACAASLLTFFSGFGLGTLLTPVFMLCGFDLQISI
                     ALTAVVHLLNNLFKLLLTGKHFNKEVVLKFGIPAIAGAFIGSFILGNVGEPTLLYSGF
                     IGSTIVQVEFFKLIIAIIMIVFALMEIIPALNFSAEKKWLPFGGLLSGFFGGLTGHQG
                     ALRSAFLMKLGLTKESFIASGITIACMIDISRLSVYADKFAISAWQSQWQLLLTATLS
                     AFIGAIAGQQLLKKMTIRILHLSVSVLIILFSFALALGLI"
     gene            complement(6144..6446)
                     /locus_tag="PK610_05870"
     CDS             complement(6144..6446)
                     /locus_tag="PK610_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRJ35492.1"
                     /translation="MFGNMMEQLQQMKQKMEESKQRLERITVEGVSGSGKVKVTMNGN
                     RKVTGIQIDPSLLQADPEELEDLLIMAFNAALEQAESVNEAEMQSTAKGIMPNLGL"
BASE COUNT         1874 a         1403 c         1422 g         1968 t
ORIGIN      
        1 gagggcgcca tgacgcttgc agccgcactt gcctccacgc tggcgccgtc cgcgaactcc
       61 gccgcctcct caaaatctgc accgctccaa aacattaaac ggatcccttt tttttgtttg
      121 ctgtagccaa caatggggtt ttcatcaata aaccaaaccg gatgcgcatg ccagatttta
      181 ctttcctgtt ttttcagaac tgagttaatt gtgtgttcaa gaaactcgca aattttctgt
      241 tcagaatttt caagacttga atgatagaat tgaatttccg gatgcattag cagttggatt
      301 ttgtactttg aaagatacga ataggatcgg taatggtgaa atttagatag ataacgacct
      361 gatgatttgt tttagatcac ttcaagcagt ccttccagtc gcatacctct tgatcctttt
      421 ataaggatgt ggtattgttg aaagggatgt tcagcaataa atttttttgc tgctgcagta
      481 tccagaaagt gaagcagcga tgaatcttta atctgtgatc caaacaaagg tcctacaaaa
      541 gcgcctttta atccgagttg cctgctcagt tctatcatcg atagatgttc ctccggagag
      601 gcatctccca attcaagcat atcgccaata atgaagaatt tgttggatgc cttcatttct
      661 gcaaaattct caagggcaac gcgcatgctc gaagggttgg cgttgtaagc atccagaatc
      721 aaagtgttgt ttcccttttg cagaatttgt gaacggttgt tttcggggac atatttttcc
      781 agtccggctt ttatctgctg caaacttaaa ccaaagtatt ttccaacagc agcggcagcg
      841 agtgcattat cgaaattgta ttttccaacg atctgtgttt ttaccgaaat actttttccg
      901 ttttctgcta tttccatttc cagaaaggga ttcgcacctt tgtttagtcc acgcatataa
      961 tttccttcgc cgtgaccata taatttcctg ttcatgcctt ccgacatttc catcagcaca
     1021 tcggaatctg catttacaaa agccagaaaa tttccggtgc gtaaaaagtc atagagttcg
     1081 cctttacctt ttttaattcc ttcaacaccc ccaaatccct caatgtgggc ttttccaatg
     1141 tttgttatta atccgaattc gggaagtgca attctgctaa gtgctgctat ctctttttgg
     1201 tgattggcgc ccatttcgat aattgcaaat tcatgttccg gacgaatgga taataatgtt
     1261 agagggacgc caatatgatt gtttaaattt ccgcttgtag caagcgtgtt gaactgttct
     1321 gaaagcacag catgcatcaa ctctttagtg gtcgtttttc cattgctgcc tgttaaggcc
     1381 agtacgggga tttttagttg ttgacgatga tacttcgcca attcctgcag tgcggttaaa
     1441 acatcaccca ccagaaaaaa gcgattgtct tttacaacat tggggtcgtc caccactgcc
     1501 attgcgcatc cttgttcaag cgcttgttct gcaaattgat tggcgtcaaa atttggacct
     1561 ttcaacgcga aaaataaaga acccggaatg agtttacggg tatcggttac aacctgggga
     1621 tatttgcaat agtgagaata gagctgctca atcatgaagc aaatttcact acgaaacctg
     1681 cgcccaaaac cttgtttgag atagaattat taacagagga aagggtatta gtgctttaaa
     1741 aagggcaatt atttcacctt tagtttttgt ccgaccctta acacgtcgtt gcgattcatg
     1801 ccgtttagct ggcaaagttt atccacagtg gttccgtggt tgcgtgcaat aacagataga
     1861 ttgtcgccgg agcgaacagt gtagtatttt gcacctgcac tgttagaatt ttttacggtt
     1921 tgtccggttc ctgccattac aggttcgtgc gaatggcctt taggctgcgt gtttttgcga
     1981 gaaacagcgc ggtaatcgaa tagaccggga tgtataaata ggttctcgtt ctgtaaacgc
     2041 ttgttgtcaa agtcaataat ctcgtaggga tcgaacgcat tgtcgtaaaa acggcattcg
     2101 aaatgaaggt gcggaccata agatcttcct gtattaccgc caagcccgat gagttcgccg
     2161 gaatgaactt cctgatcaac ctgaacagtt attttactca aatgggcgta ataggtttcc
     2221 agtccgttgt ggtggcgaat aatcaccaga tttccgaaac cacctttgtt gtattgggca
     2281 taacgaactc ttcctgaaaa tgctgcacga acagtatcac ctacattaag gtcaatatca
     2341 accccatggt gaaagcgcga accgcggtat ttgaatttgg agaggacctg tccgttggtg
     2401 ggcatagaaa atttactgta ggtagagtcc acagtgcaaa tccaaatggt atcgtgcaga
     2461 cggctgatgt cgttttcgta ggtaaaagga atctcgttgt accaggtagt cttgaaattc
     2521 agactggtat cggtactcac cactttatgg aggtagtcgt tcatcacccc attaaaattg
     2581 gtgctcgaaa cgtacatcca ggttttatcg gcaaataaaa taatggtgct gttgttcatg
     2641 cggagggtat ccacaatctc tttgccctca taggtttccc caccctgggc cttaagcagg
     2701 ggcgatgcgg tcagaaaaat gaagaccgtc agaacccttg cgagtaatcc tgtttttttg
     2761 ctacctaaca cagttgttac ttttccttaa gcggcctgaa attagcattt ttatcgaaag
     2821 tagaaaccta aagaagtgtt aaaatccccc aaaaaggagg ctagtaaaat ggtaatttac
     2881 tgtaaaacag caatattact ttggggattg acggtagatt tcaaggagtt tttttgcagc
     2941 ttcgaacgga gaaatactca aatcagccac tttttgttcc agatccggta attgcgactt
     3001 aatatccggg tggtgaaaaa agctgtattc caactgattg aaaatactgg tgcgcaacca
     3061 gaacaggttt tgttctttcc tcgatcgttc aatccaaccg ttggagaggg tcagcgttcg
     3121 gaattcatca ataattgacc atgccttgtc aatccctttg ttttccagcg cagaacaggt
     3181 ttcaacgcgg ggtgtccagc cggattcagg ggcgggaaac agatgcaatg cagcggcata
     3241 ttcagctttt gagcggcggg tggcatcggt gtttgtgcca tcatccttat taatgaggag
     3301 caggtcggta agttccatga tgcccctttt tattccctgg agctcatctc caccaccggc
     3361 aatggttagg accaggaaga aatccaccat cgaatggact tctgtttcac tttgaccaac
     3421 acctactgtt tcaaccaaaa caatatcaaa tccggctgcc tcgcacagat acatgctttc
     3481 gcgggttttg cgcgcaacac ctcccagggc agcaccggag ggactcggtc gaatgaaggc
     3541 attgggatcg gtcgataaac tttccattcg ggttttatcg ccaagaatgg atcctttatg
     3601 cacagcgctg gtggggtcaa tggcaagtac agccaccttg aatcctttat tggtcaggaa
     3661 ttttccaaac gcttcaataa atgtgctttt tccggctccg ggtaatccgg taattccaat
     3721 tctgaatgat tttccactta agggtaagca acgttgcaat aggttggatg ctgcttcctg
     3781 cagatcttcc cgttctgatt cgatcagtgt aatagctctg cttaatgctg cacgatcacc
     3841 tttcgtaaga tcggtgaata atgtttcggt gtcaaccaac gagggtgctt ttttcctgcg
     3901 aaaagaggga ttgatatagg aagaatcatc gtgacctgac atagcgctaa attagtggat
     3961 aggattgaaa gtgccgaaat ccgaatctca tggcagggat ttatacgcat tttgccaggt
     4021 tgtatccgtt acatccttaa gcagcttaaa gagtttttct ccaatgaaaa agtcgttcat
     4081 attcttcaca ccgtattctt tttccagata tttcatttgc tcatacgatg gcgcataata
     4141 cccctttgcc caaccctcgt cctgacgaag gtgagcgttt ttgggatcgc gtttgtagcg
     4201 aattttccaa aggtcggtga tgagcggaca ccaataacct atgtatttgt cgctatgcat
     4261 ttcgtaatcg ggataacagt ctgaaattcc attctccgca aaaaaatctt tagcatcagg
     4321 attggcaaga gaagttgcat tacctgttgc ctgaagaata aacatatcca cactgatgtg
     4381 ctgtgtgcga atgatacgat tgtcctgcaa ggtgagtaca gcaaaagaag caatctgcga
     4441 attcgctgaa tttaccaatg ccattccata gcatacccta acctgcaaat cgttacccga
     4501 actgcatagt ataaaaagcg cgccaatgaa aagcagtgtt tttcgcacga acgaaagata
     4561 aggaaattta aagtccattt tcaggaggca ctatcaggtt actgttgtaa cagcaaatct
     4621 caggccctta tttctttctg aaaatgaaaa acagcaagat aacacagatg atactcatca
     4681 ctattccggg aataatgctt tttggaagaa agaaagagac caataaggcg ggcaccataa
     4741 gtatgctcaa taagtgaaag cgaccctggt gattggagtt gttaaacatg tggtttaatt
     4801 gaacctgaaa atttcaccgg gagtgatgac ggcatcaagt cggatgtcgt gtgcgttggt
     4861 gtcttcaatt aattctacag gaggaaacaa actcagtcca attttttgtg tttgcggtcg
     4921 gcatgtgctt aaaaagcggt cgtaaaaccc tttgccataa cctacacgtg aaccattttt
     4981 atcgaagcag agaagcggca ccagtacgag gtcaaaacag gaggcttcaa ctacttcacc
     5041 atcgatgggt tctggtattc cgagtttgtt tattttgagt gtggtatgca agtggtatcg
     5101 gcgatgtgtc attgttccgc tgctgaaatc acttaccggt actgcaagct gtacccccgg
     5161 aaattcgcgt tgaagctggt taatgaaata gaaggtattg atttcattgt tcctcgtaat
     5221 ggaaaggaaa atggaaacac attcttcgta aaggggaaat tcgcgaaaaa acagagaagc
     5281 aagcgactga ctactgtctt ctacctgacc tgtagttagc gaacgcatac gttcaaggta
     5341 ttctatccgt agttcttctt ttaccatgat ttaaatcaat cccaaagcca gtgcaaaaga
     5401 aaacaaaata ataagtactg aaacactcag gtgtaaaatg cggatggtca tttttttcaa
     5461 aagctgttgt ccggcaattg ctccaataaa agctgaaagc gtagcagtca gcaataattg
     5521 ccattgactt tgccatgctg aaattgcgaa tttgtcggcg taaacactta agcgggaaat
     5581 atcaatcatg catgcaatgg taattccact tgcaataaag ctctcttttg ttagccctag
     5641 cttcattaag aatgcgctac gtaaggcacc ctggtgaccg gttaatccac caaagaaacc
     5701 gctaagtagt cctccaaacg gcaaccattt tttttctgct gaaaaattca atgcaggtat
     5761 gatttccatc aatgcgaata cgatcataat aatggcaata atcagtttga aaaattcaac
     5821 ctgcacaatt gttgaaccaa taaatccgga atacaaaagt gttggttcgc caacattacc
     5881 taaaatgaat gatccgataa aagctccggc aattgcagga atgccaaatt tcaatacaac
     5941 ttctttgtta aaatgttttc ctgtcagcag taacttgaat aagttattca acaaatgcac
     6001 tactgcagta agggcgattg aaatctgtaa atcgaatccg cagagcataa ataccggtgt
     6061 tagcaatgtg cctaatccga atcctgaaaa aaaagtcagg agcgaagcag cgcaggcggt
     6121 aagaccgatc agtaaaaact cgttcataat ccaaggttgg gcatgatgcc tttcgctgtc
     6181 gattgcattt cagcttcatt tacactttct gcttgttcca atgcagcgtt aaaggccata
     6241 ataagcaagt cttccaattc ttcagggtcc gcttgaagta aggacggatc aatctgtatg
     6301 ccggttactt ttctgtttcc gttcattgtt actttcactt taccggagcc ggaaacgcct
     6361 tcaacggtaa tacgctcaag acgttgtttc gattcttcca ttttttgctt catctgctga
     6421 agctgctcca tcatattacc gaacatatag cgatttttta caaatttaga taaatacgtt
     6481 taattggaaa atggaattga taaaagtgat cgacgattaa gatagattta acaaggagaa
     6541 attcaactat ttttgataaa gctatactac cgaactataa aatcgtttta atttttcagt
     6601 aattgattcc ataccgaatt tttcattcat ttcagatttc attttctgtt tgtcgtaagc
     6661 gttatgg
//
LOCUS       DAOMYG010000139         6662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3910_length_6662_cov_7.776903, whole genome shotgun sequence.
ACCESSION   DAOMYG010000139 DAOMYG010000000
VERSION     DAOMYG010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3910_length_6662_cov_7.776903"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..535)
                     /locus_tag="PK610_05875"
     CDS             complement(146..535)
                     /locus_tag="PK610_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRJ35493.1"
                     /translation="MSTFSKSERLHRKKIIEQLFTKGGQSMLAHPVRMVWQDADLEDV
                     VQVMFSAPKRNFKKAVDRNRIKRIMREAYRLNKSELRKCCEEKQKKIAVAFLFVGKEM
                     PKGQEVRDKIIVLLQRLNKELETNSAE"
     gene            complement(622..1368)
                     /locus_tag="PK610_05880"
     CDS             complement(622..1368)
                     /locus_tag="PK610_05880"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRJ35494.1"
                     /translation="MAIKSILISQPAPETEKNPYADLSKRFKLKVDFRPFIHVEGVDS
                     KEIRKDKVSLPEHTAVILTSRNAVDHFFRVCKDIRYTVPDTMKYFCISESTAYYLQKY
                     VVYRKRKIFHGKQKFIELLDVIKKHKEENFLLPCSDIHKQEITDVLDQHNIKYTKSVM
                     YKTVASDLSDLADIKYDMLVFFSPQGIESLLQNFPKFKQNKTLIAAFGATTAAAVKTA
                     GLRLDLQAPMPNAPSMTMAIEHFIKNHKEK"
     gene            complement(1401..2216)
                     /locus_tag="PK610_05885"
     CDS             complement(1401..2216)
                     /locus_tag="PK610_05885"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HRJ35495.1"
                     /translation="MPENIPIGTLPDTSQSVGWLPAVQPVTKDQLSVIKEKMSAGFQW
                     GSSLHPDKIDQSESVVVFGVLMLLLITFVVFRIVYSNRFNQLAETFISPRYMRQALRE
                     ELSLTHPFTLVMLLQYAVCAGLLIYRFNIRIPAHVYPADGIVQASIFSGIIFGLIISR
                     AILYRFIQFLIGEDGGLTENRYSMVLFTEVTGIFILPFTILAWFGPRDYIIPLIVFCG
                     AIGLIIYIFRIIRGFLGGLNSGTAGVYIFLYFCALEILPLIVLVRYISDRYQG"
     gene            2302..3504
                     /locus_tag="PK610_05890"
     CDS             2302..3504
                     /locus_tag="PK610_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRJ35496.1"
                     /translation="MAKTDLFQAPDYYQLDELLTDEHKMVRDAARAWVKKEVSPIIEE
                     HYEKATFPNHLLKGLAEIGAFGPYIPTEYGGAGLDQISYGLIMQELERSDSGLRSTAS
                     VQSSLVMYPIYKYGNEEQRKKYLPKLASGEWIGCFGLTEPDHGSNPGGMITNFKDAGD
                     HVILNGAKMWISNAPFAQIAVVWAKDESGRIHGLVVERGMEGFSTPEMHGKWSLRASA
                     TGELIFNNVKVPKENILPGRSGLGAPLSCLDSARYGIAWGAIGAAMDCYDSALRYSLE
                     RTQFDVPIGAFQLTQKKLAEMLTEITKAQLLTWRLGVLRNDGRATSAQISMAKRNNVH
                     MAITIAREARQIHGAMGITNEYSIMRHMMNLESVITYEGTHDIHLLITGMDVTGIPAF
                     KTAPLEKK"
     gene            3575..3847
                     /locus_tag="PK610_05895"
     CDS             3575..3847
                     /locus_tag="PK610_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35497.1"
                     /translation="MKAAFYFVVLVFLPWVLIFWFFKRFERGTKKALLHLLTMLALQG
                     VFFLGAYFSDDQSSVMLWTITSTFFYTAHTFIFFVHGLLKYLDTID"
     gene            complement(3877..5088)
                     /locus_tag="PK610_05900"
     CDS             complement(3877..5088)
                     /locus_tag="PK610_05900"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRJ35498.1"
                     /translation="MQLKKFLYLIADLGSNPFTDPGKSRLYRAANLFVLNALFWMLYS
                     FLWDLFDNAEDDSIGIYVLPWIMAFQLFVLWLNRKGNTFLALTLLNFSSSSMLVFFAI
                     RAGEEAGIHFHFISLIIGTCIMFAYKEARNYFILNLAFALVGFILLIYGFKNIPPEYV
                     QSPEELIITRRVHFFIVMFVTVFFSFIVLVIFNRQEKELRKALEEKETLLSEVFHRVK
                     NNMAVILGLINLKKNQSHSEEAVEALLQCHMRVMSMAMVHNRIYENKSLNNIQLDIYL
                     SELSEEVLNSLNAENLIELKKDLHPTKSDISKAIPIGLIVNEVITNAFKHGFKSHSGK
                     LEIHLHPNTETNGKAILIIKDNGPGIALEKTANTDSLGMSLIYSLSEQIDGECNFHNE
                     DGLVFKLTFPY"
     gene            complement(5092..>6662)
                     /gene="hemC"
                     /locus_tag="PK610_05905"
     CDS             complement(5092..>6662)
                     /gene="hemC"
                     /locus_tag="PK610_05905"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRJ35499.1"
                     /translation="LNTMKNITIGSRGSDLALWQARFIQLELRRIGIESTIEIISTRG
                     DEIQNLSFDKMEGKGFFTKEIEEALLNKKIDLAVHSHKDLETTSPEGLTVAAVSYRED
                     PRDLLLIRKDCTDIRELFNIKYNAKVGTSSARRKSQLMAHRPDLDLADLRGNVPTRIQ
                     KLREGQYDAIMLARAGVHRLEIDLSEFHVELLDPRVFIPAPAQGVLGLQVRNTDTDLI
                     EALKKIHHKDVEVLIESERSVLRKLEGGCQLPLGVYCIKENDIYKLWVSHASQWKGIP
                     RRFYLEGTDAHLLSEEVLKKINSKTPKRIFITRNLKLTDYFHRALSANGYAVKGASLT
                     RYQEVPFTGLPDCDWIFFSSKNCVKFFFAQEPKIPSGVKIGSIGGATAEALKKRGIKP
                     DYIGQSNDTVVIGKEFANMVRGQHILFPQSTASFRTVQKQFADQRSLTDLIVYDTIPD
                     EEAEIADSDVLVFTSPTNAMLYLRKKKITEKHTVIAIGKSTAEELKQKGNSNIHLPWN
                     TTELAMADAVTTLN"
BASE COUNT         1888 a         1387 c         1405 g         1982 t
ORIGIN      
        1 tctttgtata cagcaccaaa aatttcaaga ttttttgtga tctcaaattc attgctgttt
       61 tgcggacgaa acgataaacc gatgaatcct gtaaagagga tcaaagcgcc ggatataaaa
      121 agggttctgg ttttcatatg caatcttatt ctgctgaatt tgtttccagc tctttattca
      181 aacgttgtaa aagtacaatt attttatccc tcacctcctg acctttgggc atctccttgc
      241 caacaaacag aaaggcaaca gcaatttttt tctgcttttc ctcacagcat tttcgcagct
      301 cagatttgtt taatctgtag gcttctcgca taattctctt gatccggtta cggtctacag
      361 cctttttgaa gtttcgttta ggggcactga acatcacctg tacaacatcc tccagatctg
      421 cgtcctgcca aaccatacgc accgggtgag ccagcatcga ttggcctcct tttgtgaaaa
      481 gctgttcgat gattttcttt cggtgaagcc gctcggattt tgaaaaggtg gacaaggttt
      541 tttttttaca agttacaaca tccccctctc cggagtagat tcttgataaa gtaaatgtga
      601 acggaagatt gttggagaag attatttctc cttgtggttc ttgatgaaat gttcaatcgc
      661 cattgtcatg ctcggagcat tgggcatcgg cgcctgaaga tcaagacgaa gtcctgccgt
      721 ttttaccgct gctgcagtgg tagccccaaa ggctgcaata agggttttgt tttgcttgaa
      781 ttttgggaaa ttctgcaaca atgattcaat tccctgtgga gagaaaaaaa ccagcatgtc
      841 gtacttgata tctgcaagat cactcaaatc acttgctacc gttttataca ttaccgactt
      901 ggtgtacttg atgttgtgct ggtccagtac atcggtaatt tcctgtttgt ggatatcaga
      961 gcagggcaac agaaaatttt cttctttatg tttcttaatg acatccagaa gctcaatgaa
     1021 tttttgttta ccgtgaaaaa tctttcgctt acggtaaaca acatatttct gcagataata
     1081 ggctgtcgat tcgctgatgc aaaaatactt catggtatct ggcacggtgt agcgtatgtc
     1141 tttacatacc cgaaagaaat gatcaaccgc attacggctg gtaagaatca ctgcggtgtg
     1201 ctcaggcaaa ctgactttat ccttacggat ctctttgcta tcgacgcctt caacatgaat
     1261 aaaaggcctg aagtccactt tcagcttaaa gcgcttactg aggtccgcat acggattctt
     1321 ctccgtttcc ggtgccggct gcgagatgag tatggatttt atcgccaatg ctgtgagatt
     1381 taaaccatcc ttaaaatctg ttacccctga taccggtcgg atatgtaccg taccagcact
     1441 atgagcggca ggatttcaag ggcgcaaaaa tacaaaaaaa tatagacgcc tgccgttccg
     1501 ctgttaagcc cgcccaaaaa acccctaata attctgaaaa tatagattat cagtcctata
     1561 gcgccacaaa aaacaatcag gggaatgata taatccctgg gcccaaacca tgcaaggatg
     1621 gtaaagggga gtatgaaaat tcctgttact tcggtgaaaa ggaccatgga gtaacgattc
     1681 tctgtaagcc cgccatcctc accaatcaaa aactgaatga aacggtaaag gatcgcacga
     1741 gaaatgatca gtccgaaaat gattccgctg aagatggaag cctgcacaat accgtctgcc
     1801 ggataaacat gtgcgggtat tcgtatgtta aacctgtaaa tcaaaagccc agcacaaaca
     1861 gcatattgca acaacataac caacgtgaac ggatgggtca gggaaagttc ctcccgaagc
     1921 gcctgccgca tgtatcgggg tgaaataaaa gtttcggcaa gttgattgaa ccggttggaa
     1981 taaacaatcc gaaaaacaac aaatgttatc agcaaaagca tgagaacacc aaagaccaca
     2041 acactttctg actggtcaat tttatcggga tgaagggaac tgccccactg gaaaccggca
     2101 ctcatttttt ccttgatcac actcaattga tcctttgtta ccggctgtac tgcaggtaac
     2161 cagccaaccg attgggaggt gtcgggtaaa gtgccgatag gaatgttttc tggcattcag
     2221 caaaagtaga caaagacctt catcatcagg aaggaagtcg actgtgattt tttacctaat
     2281 ttcgcaccaa aaccgttaaa aatggccaag accgatcttt ttcaggcacc cgattactac
     2341 cagctcgatg agttgctgac cgatgaacat aaaatggttc gtgatgcagc aagagcatgg
     2401 gtaaaaaaag aagtttctcc catcatagag gaacattacg aaaaggcgac ttttcccaat
     2461 catttactca aaggactcgc tgagatcgga gctttcggtc cttacattcc aacggaatat
     2521 ggtggagcag gtctcgacca gatttcatac ggattaatca tgcaggaact cgagcgttca
     2581 gacagtggtt tgcgttctac agcttcagtg caaagctcat tggtgatgta tccgatctac
     2641 aaatacggaa atgaagaaca gcgcaaaaaa tacttgccca agttggcttc cggtgaatgg
     2701 attggatgtt tcggattaac ggaacctgat catggttcga atccaggtgg aatgatcacg
     2761 aattttaaag atgcaggtga tcatgtaatc cttaacggag ctaaaatgtg gatttccaat
     2821 gctccatttg cgcagatcgc tgtggtttgg gcaaaggacg aaagcggaag aattcatgga
     2881 ttagtggttg aacgtgggat ggaagggttc tctacacctg aaatgcacgg taaatggagt
     2941 cttcgtgctt ctgcaaccgg agaattgatc tttaacaatg taaaggttcc aaaagaaaat
     3001 attcttccag gaagatccgg tcttggcgct ccgttgagtt gtctcgattc ggcgcgttac
     3061 ggaattgcct ggggtgctat tggtgcagca atggattgtt acgattcagc tttgcgctat
     3121 tcgcttgaac gtacacagtt tgatgttccc atcggagcct tccagttaac ccagaaaaaa
     3181 ctggcagaga tgcttacaga aataaccaag gcgcaattgc tgacatggcg tttaggtgtg
     3241 ctgcgaaatg atggtcgcgc aacttctgct cagatatcaa tggcaaagcg gaacaatgtg
     3301 catatggcaa ttactattgc acgtgaagcc cgtcagatcc atggtgcaat gggtattacc
     3361 aacgaatatt ccattatgcg tcatatgatg aatctggagt ctgttatcac ctatgaagga
     3421 acacatgata ttcacctcct tattacagga atggatgtta ccggtattcc tgcttttaaa
     3481 acagctccgc tggaaaaaaa ataattttct gaatcaaaaa atcccgtggt attccgcggg
     3541 atttttttgt ttaccattac gaatctgttc actcatgaaa gctgcttttt actttgttgt
     3601 tttagtcttt ttaccctggg tgctgatttt ttggtttttt aaacgctttg agagaggaac
     3661 aaaaaaggcc ttacttcatt tgttaaccat gttggcattg caaggagtgt tttttttggg
     3721 agcctatttt tccgatgatc agagttcggt aatgctttgg accattacct caactttttt
     3781 ttacacggca catacattta tttttttcgt gcacggattg ttaaagtatc tcgatacaat
     3841 tgactgaagc tgcacctaaa aaggctctcc gctattctaa taggggaaag tcagcttaaa
     3901 gaccaatcca tcttcattgt ggaagttgca ctctccatca atttgttcgc tgagggaata
     3961 aatcaaactc attccaagag aatcggtatt agcagttttt tcaagcgcaa ttcccggacc
     4021 attatcctta attatcaaaa tggcttttcc atttgtttct gtgttaggat gcaaatggat
     4081 ttcaagtttt cccgaatgcg atttaaagcc atgtttaaaa gcattggtga ttacctcatt
     4141 aacgataagt ccaatcggaa tcgctttgga gatatcgctt tttgttggat ggagatcttt
     4201 ttttaactca atcagatttt ctgcattcag cgagttcaac acctcctcgg aaagttcact
     4261 taggtagatg tcgagttgaa tattgttaag ggatttattt tcgtagattc tgttatgcac
     4321 cattgccatc gacatcaccc gcatatggca ttgcagtaat gcttcaacag cttcttctga
     4381 atgagattga ttttttttca ggttaattaa cccaagaatg acagccatat tgttctttac
     4441 cctgtgaaac acttccgaga gcagggtttc tttctcttcc agcgccttac gcagttcttt
     4501 ttcctgcctg ttaaaaatta ccagtacaat aaaagagaaa aacaccgtaa caaacataac
     4561 aatgaaaaaa tgaactctcc gggtgataat taattcctct ggtgattgaa catattccgg
     4621 ggggatattt ttaaagccat aaataagaag gataaatcca accaacgcaa atgccagatt
     4681 cagaataaaa taatttctgg cctctttgta agcgaacatt atacatgtac caataataag
     4741 cgatatgaaa tggaagtgga tgccagcttc ttctcctgct cgtatcgcga aaaacacaag
     4801 cattgaagat gatgaaaaat tcaatagagt aagcgcaaga aatgtatttc cctttctgtt
     4861 cagccacaat acaaaaagct gaaaggccat gatccatggt aaaacataaa taccaattga
     4921 atcatcttct gcgttatcaa ataaatccca caaaaaggag tagagcatcc aaaacagagc
     4981 gttcagcaca aataagtttg ctgcacggta caaacgtgat ttgcccggat cagtaaatgg
     5041 atttgatccg aggtcggcaa tgaggtaaag gaattttttt aattgcacag attaattcaa
     5101 agtggttact gcatcggcca tggcaagttc tgtggtgttc catggtaaat gtatgttgga
     5161 gttgcctttt tgctttaatt cttctgcagt tgatttacca atggcaatta cagtgtgttt
     5221 ttcggtgatc ttctttttgc gcaggtaaag catggcgttg gttggactgg taaataccaa
     5281 tacgtcgctg tccgcaattt ccgcttcttc atcaggaatg gtgtcataaa caatcaagtc
     5341 agtgagtgaa cgctgatcgg cgaattgttt ttgtacagtt ctgaaacttg cagttgattg
     5401 tggaaataaa atgtgttgac cacgaaccat atttgcaaat tcctttccga ttacaacagt
     5461 atcgtttgat tgtccgatgt agtcgggttt tattcctctt ttcttcagtg cttcggcagt
     5521 tgctccccca atactcccaa tctttacacc ggaagggatt tttggttctt gtgcgaaaaa
     5581 gaattttacg cagtttttag atgagaaaaa gatccagtca caatcgggca gaccggtaaa
     5641 tggcacttcc tgataacgtg ttagtgaagc gcccttgaca gcataaccat tcgcacttaa
     5701 tgcacgatga aaataatcgg tgagtttaag attgcgggta atgaaaatac gtttcggtgt
     5761 tttgctgttt atttttttta gcacttcttc actcagcaga tgtgcatcag ttccttccag
     5821 ataaaatctt cttggaattc ctttccattg actggcatga gaaacccaca atttataaat
     5881 atcgttctct ttaatgcaat acacacctag cggaagttga catcctcctt cgagttttct
     5941 cagaacagaa cgttcgcttt caattaatac ctcaacatct ttgtgatgga tttttttaag
     6001 cgcttcaatg agatcagtgt ctgtgttgcg aacctgtaat cccaatacgc cctgtgcagg
     6061 tgcggggatg aatacacgtg gatccagtaa ttcaacatga aattcactca agtcgatctc
     6121 caaacgatgt acgcctgcgc gcgcaagcat tatagcatcg tactgacctt cgcgtaattt
     6181 ttgaatgcgg gtaggtacat tccctcgcag atcggcaaga tccagatccg ggcggtgggc
     6241 catcagttgc gattttcttc tcgcagatga tgtgcctact ttggcattgt atttaatatt
     6301 gaacaattcc cggatgtctg tgcaatcctt gcggatcagt aatagatcac gagggtcttc
     6361 ccggtaggag actgccgcaa ctgttaaacc ttcaggagat gttgtttcga gatccttgtg
     6421 ggaatgtacg gccaggtcaa tttttttgtt caacaacgct tcttcaattt ctttcgtgaa
     6481 aaaacctttg ccttccatct tatcgaaact cagattctgt atttcatccc ccctggtgct
     6541 aatgatttca atagtggatt caattccgat ccgacgaagc tcgagttgaa tgaaacgagc
     6601 ctgccaaagt gcaagatcgc ttccgcgtga accaatagtt atgtttttca tagtattcaa
     6661 aa
//
LOCUS       DAOMYG010000140         6607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3960_length_6607_cov_8.567766, whole genome shotgun sequence.
ACCESSION   DAOMYG010000140 DAOMYG010000000
VERSION     DAOMYG010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3960_length_6607_cov_8.567766"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PK610_05910"
     CDS             <1..717
                     /locus_tag="PK610_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylbenzoic acid--CoA ligase"
                     /protein_id="HRJ35500.1"
                     /translation="VRPDSNPAEHLFAEQSFDFAAMIPLQISNALESNEGKQKLNAIK
                     TIIIGGGESSERLIRKLQGLQCSCYATYGMTETVSHIALRKLNSQEKSESYTLLKGIR
                     VDVDDRSCLIIDAPHLHDERLITNDVVEFTDLNKFRWLGRADHVINSGGLKIHPEQVE
                     KQIAEIITDRRFYLSAKPDDVLGQRPVLVLEGDILDKHTEKMLLSHLKESLPRNQSPA
                     EIVYINTFKETPTGKVIRSI"
     gene            805..2640
                     /locus_tag="PK610_05915"
     CDS             805..2640
                     /locus_tag="PK610_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HRJ35501.1"
                     /translation="MFRKLLSVSLLSFVLFACGGNKTASENPEQNNQSEIQKEVQIYL
                     DEYNQKFQELLYAQNEAQWAMLTRIVPGDTTASHNAGLADEAMSKFTGSKANTDKAQN
                     YLTKKSELSEIQVRQLDYILFLAGGSPEAAGDIVKERIKAQNEQSEKLYSFNLTIDGK
                     KVSTNDIDAILKTENNVAKRLKAWSASKEVGKGLKAGLVKLQDLRNRSVQALGYSDYF
                     SYQVSEYGMSSEELRGVCQDMIRELWPLYRELHTWARYELAARYKQPVPEMLPAHWVP
                     NRWGQDWSGMINVEGFDVEGALEKKSAEWIMQEGEAFYVSLGFESLPKSFHEKSSLYP
                     LPKDANYSKNNHASAWHMNNDRDVRSLMSVEANSEWWETVLHELGHIYYFLEYSNPDV
                     PIILRNGANRAYHEAFGTMIGLASMQKSFLQGRGLIDTNAESDPTQVLLREALSYVVV
                     IPWAAGVMTDFEYELYSKNLPADQYNKRWWELVGKYQGIVPPTTRGEEYCDAATKTHI
                     SDDPAQYYDYAMSNILLFQFHEHIATKILKQDPHNTNYWGNKEVGNFMKELMRPGASV
                     DWKDHLKKNIGSEMSAKAMVNYFSPLMDYLKKVNEGRTCTLPETI"
     gene            complement(2686..4548)
                     /locus_tag="PK610_05920"
     CDS             complement(2686..4548)
                     /locus_tag="PK610_05920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ35502.1"
                     /translation="MEKFDLTEKAVERRIVKIEIMRTIFLVFLVVTIFSARINAQAVY
                     QLNINNINAAIYSQGGLFSPYETQGFEAPGGSGLSTIFASSFWLAGYDSDSLNFLRAN
                     IETYDSFMDLTTGPYQFGMDSTTVNSLAAIYNRVYPIKRAEVINFQVNFGTPGYQIPQ
                     NILDWPGTYSFNSLNYVIAPFHDQNQDGIYNPYDGDYPKMKGDEAVFIVRNDFAPHVC
                     SGGTGVGSEYHFLIYAFNDPIVASLNNTVFAEVTIINRGSVSLENAFLGCWTDLDIGN
                     AEDDFIASNVAGGYYYGYNGDTFDDPGSGQYGYGNNPPVQAVVFLGGPKYASDGLDNG
                     IDTDPQSWPDAIPYSGVGAFFDDGIIDNERMGMTGFRHYFLNDQSVSPATGGPNNPDS
                     YYKYLSGKWLDGTPLFYGGTGHLSSIGAQNNGLTPCNFAFPGSSDPYWWNTNGQPMVQ
                     TPIWSEELMMNTPSDRRGVGTCGPFNFLPGDTVVFEVAYVFAWDSLGVHTGGSLGLVD
                     DYVNEVRELWTYDTTTLYESFAVNTSEIEHDFNPDLIGLFPNPSMDFVNIDCSIVKGK
                     VRIEIFDIGGSLLFSKLDLGSIITVDVVDFTPGYYLVRVSTSQWQTEKKLIIIK"
     gene            complement(4724..>6607)
                     /locus_tag="PK610_05925"
     CDS             complement(4724..>6607)
                     /locus_tag="PK610_05925"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRJ35503.1"
                     /translation="DNSFGLLTVCMNITHTWDADLDVQLVSPGGMAILLFSGVGGSGD
                     DFLNTCLDMSAPNPIGSAGAPFTGSFRPMGMLGNFNNGSNGNGVWKLRITDTYPADQG
                     NLLDWSISFGANPPQPFPFTDSDLPLIVITTNGQTILNDPRIVCDMGIIDNGPGNRNA
                     LTDMYNNYNGRIAIEIRGSSSQSFPKKSYAFETQNLSGANLNVPLLGMPAENDWILYA
                     PYTDKTMMRDALAYRLGRDLGAYAPRYAFAELFINGQYMGVYCLEEKIKRDNNRVNIA
                     TLTTTDTTGNELTGGYILKIDRTDGPGSYFTSAYPGNFGNINFVYSDPEGHELHPLQK
                     NYIETTIDHFEDVLAGPQFMHPQNGYRKLIHVPSFIDYFLVNELSNNVDGFRLSTFLY
                     KNKNSIDSLIHMGPLWDYNLGFGNADYCSGASTSGWSYIHSGNCGDVPFWWDRFMQDS
                     TFVRELRCRYEELRQTFFSASYLNGFIDSVAQVLNESKDRNYLAWPILGTYVWPNPFV
                     GNTYQQEIVYMKSWINSRLNWMDANIPGACPNLGLAEHSSALYLALFPNPANTQITFA
                     FSSSTVITDLFIYDRLGKLLKQQQFSGSQLTLSVEDLSPGIYFVQWNSGSNQGVQKFV
                     ISR"
BASE COUNT         2163 a         1436 c         1278 g         1730 t
ORIGIN      
        1 gttcgtcccg actcaaaccc tgctgaacat ttatttgctg aacaatcttt cgattttgcg
       61 gcaatgattc cccttcagat cagcaatgcg ctggagagta acgaaggcaa acaaaaatta
      121 aatgcaataa aaaccataat tatcggcggc ggagaaagtt ccgaaagact tatacggaag
      181 cttcaaggac ttcaatgcag ttgttacgcg acctatggta tgacagaaac agtttcgcac
      241 atcgcattac gaaaacttaa cagtcaggaa aaatcggaat cgtacaccct gctaaaaggg
      301 attcgcgtgg atgtggatga tcgatcctgc ctgattattg atgcacccca tttgcatgat
      361 gagcgtttga tcaccaatga tgttgtagaa tttaccgatc tcaataagtt tcgctggctg
      421 ggtcgtgcag atcatgtaat taattcggga ggactaaaaa ttcatcctga gcaagtggaa
      481 aaacaaattg ctgagataat tacagacaga cgcttctacc tcagcgcgaa acctgatgac
      541 gttttaggtc agcgtccggt tctggtattg gaaggagaca tccttgacaa acacaccgaa
      601 aagatgctgc tcagccattt aaaagaaagc ctgccgagga accaatctcc ggctgagatc
      661 gtgtatatca acacattcaa ggaaacgccg acagggaaag tgatcaggtc gatctgagta
      721 aattttacca gtggaagaat aagcggacag acggacatca ttcaatcggt tttatttcta
      781 atattgaaaa ataaccttga aaccatgttc agaaaactat tatcagtcag cctgctttct
      841 ttcgtgcttt tcgcttgcgg aggaaacaaa acagcatccg aaaatccgga gcaaaacaat
      901 cagtcggaaa ttcagaaaga agtccagatt tatctcgacg agtacaatca gaaatttcag
      961 gaactgctgt acgcacagaa cgaagcacaa tgggcaatgc taaccagaat cgttcccggc
     1021 gacaccaccg catcgcacaa tgcaggactt gcggatgaag ccatgagtaa attcactggc
     1081 agtaaagcga atacggataa agcgcaaaac tatcttacaa aaaaatcaga actcagcgaa
     1141 attcaggttc gtcagctcga ctatattctt tttttggcag gaggttctcc tgaagctgcg
     1201 ggtgatatag tgaaagaacg catcaaggcg cagaatgagc aaagtgaaaa attgtacagt
     1261 ttcaatctta cgatcgacgg aaagaaagtt tccacgaatg acattgatgc catccttaaa
     1321 acagaaaaca atgttgccaa acgtctaaaa gcatggtcag cttcgaaaga agttggaaaa
     1381 ggattaaaag caggtctggt aaaattgcag gatcttcgca accggtcggt tcaggcattg
     1441 ggttattccg attatttcag ctatcaggtt agcgaatacg gaatgagttc agaagaatta
     1501 cgcggtgttt gtcaggatat gattcgcgag ttatggccgc tttaccgtga attacacacc
     1561 tgggcaagat atgagcttgc agcacgctac aaacaacccg tacccgaaat gctacccgca
     1621 cattgggtac ccaaccgttg gggtcaggac tggagtggaa tgattaacgt ggaaggtttt
     1681 gatgtggaag gtgcacttga aaaaaaatcg gctgaatgga tcatgcagga aggcgaagcc
     1741 ttttacgtat cactcgggtt tgaatcacta ccgaaaagtt ttcatgaaaa atcaagtcta
     1801 tatccattgc caaaagacgc caattacagc aaaaacaatc atgcatcagc atggcacatg
     1861 aacaacgacc gcgacgttcg ctcattgatg agtgttgaag caaacagtga atggtgggaa
     1921 acggtgctac acgagttggg acatatttat tattttctgg aatattccaa tccggatgta
     1981 cccatcattc tgcgcaacgg agccaacaga gcctaccatg aagcatttgg aactatgatt
     2041 ggattagctt cgatgcaaaa atcattttta caaggacgcg gactgatcga taccaatgca
     2101 gaaagtgatc ccacacaagt attgcttcgt gaagcgctca gctatgttgt ggtaattccg
     2161 tgggctgcgg gtgtaatgac tgattttgaa tacgaactct attcaaaaaa tctgcctgcc
     2221 gatcaataca acaaaagatg gtgggaacta gtaggaaaat accagggaat agttcctcca
     2281 acaacacgcg gagaggaata ttgtgatgca gcaaccaaaa cgcatatcag tgatgatcct
     2341 gctcaatact acgattacgc aatgagtaat attcttttgt ttcagtttca tgagcacatt
     2401 gcaacaaaaa ttctgaaaca agatccacac aacaccaatt actggggcaa caaggaggtt
     2461 ggaaatttca tgaaagaact gatgcgtcct ggagccagcg tagattggaa agatcattta
     2521 aagaaaaaca ttggcagcga aatgagcgcg aaagcaatgg taaactattt ttctccgttg
     2581 atggactatc tcaaaaaggt gaacgaaggc cgaacctgta ctttaccgga aacgatttaa
     2641 atccaaaaaa aataaaaatc cccgcttcac tcagcgggga tttttttatt tgattatgat
     2701 taattttttc tctgtttgcc attgacttgt tgagacacga acaagataat aaccaggagt
     2761 aaaatcaaca acatcaactg taataattga acccaaatct aacttactga acaataaact
     2821 tcctccaata tcaaatattt ctattcgtac ttttcctttg acgattgaac aatcaatatt
     2881 tacaaaatcc atggacgggt tggggaatag gcctattaaa tctggattaa aatcatgctc
     2941 aatttcactt gtattcacag caaaagattc atacaatgtt gttgtgtcat atgtccataa
     3001 ttctcttact tcatttacgt aatcatctac aagtcccaaa gaacctccag tatgaactcc
     3061 taaagaatcc caggcaaaaa cataggcaac ttcaaacaca actgtatccc cgggtaaaaa
     3121 attgaaagga ccacatgttc caacccctct tctgtcagaa ggcgtattca tcattaactc
     3181 ttcactccag attggagttt gaaccatggg ttgaccattt gtattccacc aataaggatc
     3241 tgaacttcca ggaaaagcaa agttacacgg cgttaatcca ttgttttgag ctcctatcga
     3301 tgacaaatgt cctgttcctc cgtaaaaaag cggtgtccca tccaaccatt tacctgataa
     3361 gtatttataa tagctatctg gattatttgg tcccccagtt gcaggagaaa ctgattgatc
     3421 atttagaaaa tagtgtcgaa aaccagtcat ccccattctt tcgttatcaa ttattccatc
     3481 atcgaaaaac gctcctaccc cagaataggg aattgcatcc ggccaagatt gaggatcagt
     3541 atctattcca ttatccaaac catcagaagc atatttaggc ccaccaagaa aaacaactgc
     3601 ctgtacaggc ggattatttc cataaccata ttgcccggaa cccggatcat cgaaagtatc
     3661 gccattatac ccataataat atccacccgc gacattgctt gctatgaaat catcttcagc
     3721 attaccgata tctaaatctg tccaacaacc caaaaaggca ttttccaatg aaacacttcc
     3781 acgattaatt atggttacct cagcaaaaac tgtattgttc aacgaggcta ctattggatc
     3841 attaaatgca taaatcaaaa aatggtattc cgatccaact ccggtccccc cggaacatac
     3901 atgaggcgca aaatcattcc tgacaataaa aacagcttca tcacctttca ttttaggata
     3961 atctccatcg tagggattat aaattccatc ttggttttgg tcgtggaaag gagcaatgac
     4021 ataattaagt gaattaaaac tataagtccc cggccaatcc aaaatattct gaggaatctg
     4081 atatccgggt gttccaaaat tcacctggaa attaattacc tcagccctct taattggata
     4141 aacccgatta taaatcgcag ccaaactatt aacagtagta gagtccatgc caaactgata
     4201 tggccctgta gtcaaatcca taaagctgtc gtatgtttca atatttgccc tcagaaaatt
     4261 taaggaatcg gaatcataac cagcaagcca aaaactagaa gcgaaaatgg tgctcaaccc
     4321 acttccaccg ggggcttcaa atccctgcgt ttcataggga ctaaacaagc ctccttggct
     4381 ataaatagcc gcatttatat tgtttatgtt tagctgataa actgcttggg catttattct
     4441 tgctgaaaaa atcgttacaa caagaaatac aagaaaaatc gtcctcataa tttcaatttt
     4501 cacaattcta cgctctactg ctttttcagt caaatcaaat ttctccaatt attaacaggt
     4561 ctaagaaaaa aacagcccct atatcctaag caattatttc caataaacga tctgattcaa
     4621 tttagaatcc ggaattattt aacaggtccc aaccagcaaa tacccagtca aaaaaatatt
     4681 aagtgagcaa taaagttgat aaatgcactc attaacaggt ggcttacctg ctgatcacaa
     4741 atttctgaac accctgattc gatccggaat tccattgaac gaaataaata ccgggtgata
     4801 aatcctctac cgaaagtgta agctgacttc cgctaaactg ctgttgtttc aacaatttcc
     4861 ccaagcgatc gtaaataaaa agatcagtga tcactgttga ggaagaaaat gcaaatgtaa
     4921 tttgtgtatt tgcaggattt ggaaacaatg ctaaatacag agctgaagaa tgctctgcaa
     4981 gaccaagatt tggacaagca cccggaatgt ttgcgtccat ccaattcaga cgactgttta
     5041 tccagctttt catatacacg atttcctgct ggtaagtatt acccacaaaa ggattcggcc
     5101 aaacataagt ccccaggatc ggccatgcca aataatttct gtcttttgac tcattcaata
     5161 cctgggcaac tgagtctata aagccattca ggtatgaagc actgaaaaaa gtctgacgca
     5221 attcctcata cctgcaacgc agttcacgta caaaagtgct gtcctgcata aatcggtccc
     5281 accaaaaggg aacgtcacca caatttccgc tatgaatata gctccatcct gaagtagaag
     5341 ccccgctgca gtaatcggca ttgccaaacc ctaaattata atcccacaat ggccccatgt
     5401 gaatcaaact gtcgatggaa tttttattct tatacagaaa agtgctcaaa cgaaatccgt
     5461 ctacgttatt tgaaagttca ttcactaaaa aataatcgat aaaagaagga acatgaatga
     5521 gtttacggta tccgttttga ggatgcataa attgcggacc cgccaaaaca tcctcaaaat
     5581 gatcaatagt agtttctata tagttttttt gcaatggatg caattcgtgt ccttccggat
     5641 cagaataaac aaagttgata tttccaaaat taccgggata cgccgatgta aaatacgatc
     5701 cgggtccatc cgtgcggtca attttaagaa tatagccacc ggtcaattca tttccagtag
     5761 tatctgtagt tgttagtgtg gcaatgttta ccctgttatt gtcccgttta attttctctt
     5821 ccagacagta cactcccatg tattggccat tgataaataa ttcagcaaaa gcatacctgg
     5881 gcgcgtaggc tccaagatcg cgacccaaac gataagcaag ggcatcacgc atcattgttt
     5941 tgtcggtata gggagcatac aaaatccaat cattttcggc aggcattccc agaagaggaa
     6001 cattcaggtt cgcgcctgat aaattttgtg tttcaaaagc ataacttttt ttcggaaaag
     6061 attgtgagga tgaaccgcga atctcaatgg caattcgtcc attgtaatta ttgtacatat
     6121 cggttagcgc attccggtta cccggaccat tatcgataat acccatatca caaacaattc
     6181 gcggatcgtt gagaatggtt tgtccgttgg ttgtgatcac aattagcggc aaatcagaat
     6241 ctgtaaatgg aaaaggctga ggcggatttg caccaaagct gatgctccag tccaataaat
     6301 tcccctgatc ggcaggatag gtatcggtga tgcgtaattt ccagactccg tttccattcg
     6361 atccgttatt aaaattcccc aacataccca taggacgaaa actccctgta aatggtgcac
     6421 cggctgaacc aatgggattc ggagctgaca tatcgagaca agtattcaaa aaatcatctc
     6481 cactaccccc aacaccggaa aaaagtaaaa tggccattcc gcccggacta accagctgaa
     6541 catccagatc agcatcccag gtatgggtga tgttcataca aaccgttaaa agcccgaacg
     6601 aattatc
//
LOCUS       DAOMYG010000141         6601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3966_length_6601_cov_9.865414, whole genome shotgun sequence.
ACCESSION   DAOMYG010000141 DAOMYG010000000
VERSION     DAOMYG010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3966_length_6601_cov_9.865414"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1804
                     /locus_tag="PK610_05930"
     CDS             <1..1804
                     /locus_tag="PK610_05930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ35504.1"
                     /translation="GFPELLIDKGSNKIDLIHQYSYARYVDSVQVNHFGPFRYSLMST
                     PYAHLPGKIEFLREDESDHLVYRVDDRTLIVLTRPVERFINQATSFSYLFTLFCLIVI
                     AGVVYRQFSRGFSGIHLTLQGKLQFLLVSVLLVSLVLFGLGTRYYVAGQYRAKNYNII
                     SEKIQSVRIEVTHKLGRERELSSDLRNYIGYILGKFSGVFLTDINLYDDNGLLVATSR
                     PRIFQSGLLGMQMDPEAFRQMNEERRSEFIQEERIGNMNYLSAYVPLLNDHSQLLGYL
                     NLPYFAKQNTLETELTGFLVAILNIFVVLFALSILAALFVSNWITKPLRLVQQSIAGI
                     QLGRTNKPIAYSGRDEIGSLVEEYNKKVQELERYAQELAATERESAWREMAKQVAHEI
                     KNPLTPIRLSIQHLQRSLHSDDPDWKEKLDRFTHTVIEQIDALSNIANEFSNFAKMPK
                     AEEEVLNLKSVLTNAVELYKDTPDVTVRLSRTDLQDALVYADKDQLIRVVNNLIKNAI
                     QAIPEDREGKVEISLRRNENRYLVEVKDNGSGIPHDRIDKIFTPNFTTKSTGMGLGLA
                     MVKNIVENHKGRIWFETSHNVGTSFYFTLPVHES"
     gene            1966..2748
                     /locus_tag="PK610_05935"
     CDS             1966..2748
                     /locus_tag="PK610_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRJ35505.1"
                     /translation="MSYQNLLLRSENGIAYLTINRPDKLNALNKQTISELHTAFSALD
                     KDASVRAIILTGSGEKAFVAGADISEFAAFSIDQGKALSKDGHDQLFDFVERLGTPVI
                     AAVNGFALGGGLELAMSCHVRVASNNAKMGLPEVSLGVIPGYGGTQRLAQLIGRGKAF
                     ELIFTAGMISAAEAIQWGLVNHVTTPAELLAKCEEIAFKIIKNSPSAIASAIRAVNAG
                     FADGVNGYQAEIDEFGKCFGTGDFKEGTTAFLEKRKPQFSGK"
     gene            2781..4289
                     /locus_tag="PK610_05940"
     CDS             2781..4289
                     /locus_tag="PK610_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRJ35506.1"
                     /translation="MSVLRKLASQTAIYGLSSIVARFLNYLLTPLHTSKSVFSESEYG
                     VISEMYAYVAFLIIFLTYGMETAFFRFYSKEENDKRSVFTTTFWTLFVSSSLFIFVAV
                     LFSGSIAGLLDYSNHPEYIVWFAVVVGLDALSSIPMAYLRAEGKAMRFAMVNIISVAV
                     NILLNLFFLAYCMPIVKSGNANWLTDTFYNPAIGVGYVFIANLASSIVKFILLSPEIA
                     RSFSRFSFSLLEKMLLFGLPLMIAGFAGMINETIDRILLKKILSEPLGTDGALAQVGI
                     YSAVYKLSIIITLFIQAFRYAAEPFFFAQEKEKNSGTIYARVMNYFVIVCAVIFLGVM
                     LYIDVLKHFVPNQAYWNALHIVPVLLFANIFLGIYYNQSIWYKLSGRTMYGAWIAVFG
                     AILTLVLNVILIPVFGFVGSAWTTFACYGSMMVLSYFLGRRFYPVPYKVGKVFFYLTM
                     AFAMYYGSTFIPIGWGWERAIIHSFILGVYMAMIWFIERPLKNLNSHESQSH"
     gene            4270..4701
                     /gene="dut"
                     /locus_tag="PK610_05945"
     CDS             4270..4701
                     /gene="dut"
                     /locus_tag="PK610_05945"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRJ35507.1"
                     /translation="MKVKVINRSKHSLPSYETIGSAGMDLRANIAEPIVLKPLERKLV
                     PTGLFIELPIGTEAQVRPRSGLALKKGITVLNSPGTVDADYRGEIGVILVNLSNEDFI
                     INDGERIAQMVIARHERAEWEQSESLEDSQRGAGGFGSTGV"
     gene            4701..6491
                     /gene="uvrC"
                     /locus_tag="PK610_05950"
     CDS             4701..6491
                     /gene="uvrC"
                     /locus_tag="PK610_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRJ35508.1"
                     /translation="MQTLREVVLSLPANPGVYQYFDADGKLLYVGKAKNLKKRVSSYF
                     NKEHDNARIAIMVQKITEVKVVVTETELDALLLENNLIKNLRPRYNVLLRDDKTYPWI
                     VIRREAFPRVSYTRNKDRDGSEYFGPYANTKSMHALLDLIRQMYPLRTCTLPLSQENI
                     AKQKFKICLEFQIGNCMGPCEGKQSEADYNEMIADIRRIIRGNTGDVLRELRERMKRF
                     SEEMLFEKAQIIKEKIDSLENYKSKFTVVSATVTDVDVFTIVADDKFGYVNYFKVIDG
                     AIVQSHTLEMKKMLDESNEELLRIAITELRQKFQSESREIVIPFELEYGDLVVTVPQR
                     GDKKQLLELSERNARYYMMDKHRQESVKDPERNVERIMETMKKDLRLTEQPRHIECFD
                     NSNIQGTNPVSACVVFRDGKPSKREYRHFNVRTVEGPNDFATMEEVIFRRYSRLIEEK
                     QELPQLIIIDGGKGQLGAALNSIDKLGLRGKIAVIGIAKRLEEIYYPGDNLPMYIDKR
                     SESLKIIQHLRNEAHRFGITHHRNRRSKGAVKSELLEIDGIGEKTMQDLIRKFKSIKR
                     LKEAAENEIAEVVGPAKAKILVAYFTDNKE"
BASE COUNT         1871 a         1256 c         1517 g         1957 t
ORIGIN      
        1 tggttttccc gaattgctca ttgacaaagg atctaacaaa attgatctca tccaccagta
       61 ttcttatgcc cgctatgtag atagtgttca ggtgaatcat tttggtcctt ttcgttacag
      121 tttaatgagt acgccttatg ctcatttgcc gggaaaaatt gaatttttac gcgaggatga
      181 gtcggatcac cttgtatata gagttgatga ccggacactc attgtattga ctcgtccagt
      241 tgaacgattc atcaatcagg cgacatcctt ttcgtatttg ttcacgctat tttgtctgat
      301 cgttattgca ggagtggtat atcgacagtt ttcccggggg ttttccggta ttcacttaac
      361 gctgcaaggt aaacttcagt ttctgctggt ctcggttctg ctggtatcgt tggtgttgtt
      421 tggtctcgga acgcgttatt atgttgcagg tcaataccgc gcaaaaaatt acaacatcat
      481 ttccgaaaaa atacaaagtg tgcgaatcga agtaacacat aaactcggtc gtgagcgtga
      541 gttgagttct gatctccgaa attatattgg ttatatcctt gggaaattct cgggagtttt
      601 tcttactgat attaatcttt acgatgataa tggattgctt gttgcaactt cgcgaccacg
      661 tatttttcaa agtggattgc tgggaatgca aatggatcct gaagcattcc ggcaaatgaa
      721 cgaagaacgc agaagtgaat ttattcaaga ggaacgtatt ggaaatatga attacctgag
      781 cgcttatgtt ccgctgctga atgatcacag tcagttgctc ggctatctca atcttccgta
      841 tttcgctaag caaaacacac tggaaacaga attgaccgga tttttggttg ccattctcaa
      901 tatttttgtt gtgttgtttg cattgtccat tcttgcagcc ttgtttgttt ccaactggat
      961 caccaaacca ttgcgactcg tacagcaaag tattgcagga attcagctcg gaagaaccaa
     1021 taaaccaatt gcgtattcgg gtcgcgatga aattggttcc ttggtggaag aatacaacaa
     1081 gaaggttcag gaactggagc gatatgcgca ggaacttgca gctaccgagc gcgaaagcgc
     1141 ctggagggaa atggccaaac aggtcgcgca tgaaattaaa aatccgctca ctcccattcg
     1201 gttgagcatt caacatttac aacgctcatt gcattcggat gatccggatt ggaaagagaa
     1261 actcgatcgt tttacacata ccgtaattga acaaattgat gcgctttcca atattgcaaa
     1321 tgagttcagt aattttgcaa aaatgcccaa ggcagaagag gaagtgctga atttgaaatc
     1381 ggtgcttacc aatgctgttg agttgtacaa ggatacaccg gatgtaaccg ttcgtttgtc
     1441 gcgaaccgat ttacaggatg ctcttgtgta tgcagataaa gaccagttga tcagagtagt
     1501 gaataatctg attaaaaacg ctattcaggc tattccggaa gacagagaag gtaaggttga
     1561 gatttcattg aggagaaacg aaaatcgcta tctggtagaa gttaaggaca atggctccgg
     1621 aattccgcat gatcgcatag ataagatttt cactccgaat tttaccacaa aatctacagg
     1681 gatgggactg ggtcttgcaa tggtgaagaa tatcgtggaa aaccacaagg gaagaatctg
     1741 gtttgaaacc agtcacaatg ttggcacctc cttttatttt acgcttccgg tgcacgagtc
     1801 ataatcgaac gaatactaaa ccgtaaaggg ttacttctaa aattcgaaca gaatattgct
     1861 gaagaatact cagagtgccg gtggacaagg taaaattccc ttataaaacc ttaaattcat
     1921 tcccatatct atcgaattac tacatttgcc taaacaccat ttaccatgag ttatcaaaat
     1981 ctgcttttgc gttctgaaaa cggcatcgcc taccttacaa ttaatcgtcc ggataaactc
     2041 aatgctttaa acaagcagac aatttcggaa ttgcatactg cgttttccgc attggataaa
     2101 gatgcttctg tccgcgcaat tattttaacc ggttcaggag aaaaagcatt tgttgccgga
     2161 gcggatattt ccgaattcgc tgccttttcg atcgatcagg gtaaagcgct tagtaaagat
     2221 ggtcacgacc agcttttcga ttttgtggaa cgactgggca caccggttat cgccgctgta
     2281 aatggctttg ctctgggtgg tggattggaa ttggccatga gttgccacgt tcgtgtagct
     2341 tcgaataatg caaaaatggg attgcctgaa gtgtctttgg gagttattcc gggctatgga
     2401 ggcacacagc gattggcgca gttgattggt cgtggtaaag catttgaatt gatttttact
     2461 gcaggtatga tctctgctgc agaggcgata caatggggtt tggtgaatca tgttactaca
     2521 ccggcagaac tgctggcaaa gtgtgaagag attgctttta aaatcattaa aaattctcct
     2581 tctgccattg caagcgctat tcgtgcggtt aatgccggtt ttgcggatgg tgtaaacgga
     2641 tatcaggcgg aaatcgatga attcggaaag tgtttcggta ccggtgattt taaagaagga
     2701 acaacagctt ttcttgaaaa aagaaaacca cagttttcag gtaaataatc aggaggccgg
     2761 ttttccggcc tttttcaggt atgagtgtat tacgtaaact ggcatcacaa accgcaatct
     2821 atggattgag ctccatcgtt gcccgttttc tgaattatct cctaacaccg ttacacacca
     2881 gtaaatccgt tttctccgaa agcgaatacg gggtgatcag cgaaatgtat gcttatgttg
     2941 ctttcctgat tattttcctg acctacggaa tggaaactgc atttttcagg ttttattcca
     3001 aagaggaaaa cgacaaaaga tctgttttta ccaccacgtt ctggacactc ttcgtaagca
     3061 gttcgctgtt catatttgta gcggttctgt ttagcggttc catagccgga ttactcgatt
     3121 acagcaatca tcccgaatac attgtttggt ttgcagtagt ggtgggactg gatgctcttt
     3181 cttcaattcc catggcctac ctgcgcgccg agggaaaggc catgcgtttt gcaatggtga
     3241 acattatcag tgttgctgta aatattttac tcaatttgtt ttttcttgcc tattgtatgc
     3301 ccattgtaaa gagcggaaat gcaaactggc ttaccgatac attttataat cctgctattg
     3361 gtgttggtta tgttttcatt gctaacctgg cttcgagtat tgttaagttt attttattgt
     3421 cgcctgaaat tgcgcgttct ttttcgcggt tcagtttttc attgctggaa aagatgttgt
     3481 tgtttggctt accacttatg atagccggtt ttgccggaat gatcaatgaa acgatcgaca
     3541 gaattctgct caagaaaata ctaagcgaac cactcggtac tgatggcgct ttagctcagg
     3601 tgggaattta cagcgcggtt tataaattgt caatcatcat tactctcttt atccaggctt
     3661 ttcggtacgc tgccgaaccg ttcttttttg ctcaggaaaa agaaaaaaat tccggaacga
     3721 tctacgcccg ggtaatgaat tattttgtga tcgtgtgcgc agtgattttt ttaggtgtaa
     3781 tgctctacat tgatgtgctc aaacattttg ttcctaatca ggcttactgg aatgctttac
     3841 atatcgttcc tgttttgctg tttgcaaata tctttttggg aatttattat aaccaaagca
     3901 tctggtacaa actcagcgga agaacaatgt acggtgcatg gattgcagtt tttggtgcaa
     3961 tactaacatt ggtgctaaac gtgattttaa ttcctgtatt tggctttgtt ggatcagcct
     4021 ggaccacttt tgcctgttac ggaagcatga tggttctttc ttattttctc ggtcgccggt
     4081 tttatcccgt tccctataaa gtagggaaag tttttttcta tttgaccatg gcttttgcta
     4141 tgtattatgg atcaacattt attcccatcg gatggggatg ggaaagagca attattcatt
     4201 cgttcattct gggagtgtat atggccatga tctggttcat tgaacgccct ttaaaaaatc
     4261 tgaattcgca tgaaagtcaa agtcattaat cgttccaaac attcgctccc ttcatacgaa
     4321 accatcggtt cggctggaat ggatctacgt gccaatattg cagaacctat tgtgttgaaa
     4381 ccactagaac gtaaactggt tccaaccgga ttgtttattg agttgccaat cggaacagaa
     4441 gcccaggtta gaccgcggtc cggacttgca ttgaaaaaag gcatcactgt tttgaactca
     4501 cctggtaccg ttgatgcgga ttaccgtgga gagattggtg taatacttgt taacctttcc
     4561 aatgaagatt ttattatcaa cgatggcgaa cgtattgcgc aaatggtgat agcgcgccat
     4621 gaacgtgccg aatgggagca aagtgaatca ctcgaagact cgcagcgggg agcaggcgga
     4681 tttggtagta ccggtgtgta atgcaaacat tgcgtgaagt agtcctttca ctccccgcca
     4741 atccgggagt ttatcagtat ttcgatgctg atggaaaact tctttacgtt ggaaaagcaa
     4801 aaaacctcaa aaagagagta agctcctact tcaacaagga acatgataat gcacgtatcg
     4861 ccatcatggt ccagaaaatt acggaagtaa aggttgtggt gacagaaacc gaactggatg
     4921 cgctgttact cgaaaataat ctgatcaaga atcttcgtcc gcgttataac gttttattac
     4981 gcgatgataa aacctatccc tggatcgtta ttcgaaggga ggctttcccc agagttagtt
     5041 atacccgaaa caaggaccgc gacggatcgg aatattttgg gccttatgcc aatacaaaat
     5101 cgatgcatgc actgcttgat ctcatcagac agatgtatcc attacgaacc tgtacccttc
     5161 ctttgtcaca ggagaatata gccaagcaaa aatttaagat ctgtcttgag tttcagattg
     5221 gtaattgcat ggggccttgc gaaggcaaac aatccgaagc cgattacaat gaaatgattg
     5281 cggatatccg tagaatcatc agaggaaata caggtgatgt tttgagggag ctgcgcgaac
     5341 ggatgaagcg tttcagtgaa gaaatgctgt ttgaaaaggc acagatcatt aaggagaaaa
     5401 tcgattcact ggaaaactat aaatcgaaat tcacggttgt ctctgcgacg gtaacggatg
     5461 tggatgtctt taccattgta gccgatgata agtttggcta cgtgaattat tttaaggtga
     5521 tagacggtgc cattgttcag agccatacgc tggaaatgaa aaaaatgctc gatgaatcca
     5581 acgaagaatt actgcggatt gcaataactg aactgcggca aaaatttcag agcgagtcgc
     5641 gcgaaattgt gattccgttt gagttggagt atggtgatct tgttgttact gttccgcaac
     5701 gtggggataa aaagcaattg cttgaattgt cggagcgaaa tgcacgttat tacatgatgg
     5761 ataaacaccg tcaggaaagc gtaaaagatc ctgaacgcaa cgttgaacgg atcatggaga
     5821 ccatgaaaaa agatctgcgt cttactgaac agccacgtca tatcgagtgt tttgataact
     5881 cgaatattca gggcaccaat cctgtttcag catgtgtggt gtttcgcgac ggaaaaccga
     5941 gtaaaaggga ataccgtcat tttaatgtgc gtacagttga aggtccaaat gattttgcaa
     6001 caatggagga agtgattttc cggagatatt cgcgcttaat tgaggaaaaa caggaattgc
     6061 ctcagctgat cattatcgac ggtggtaaag gtcagttagg agctgctttg aacagtattg
     6121 ataaattggg attacgaggg aagattgcag tgataggaat tgcaaaaaga ctggaggaga
     6181 tttattatcc cggagataat ttaccaatgt atatcgataa gcgatcagaa tccttaaaga
     6241 tcattcagca cctgcgcaat gaagcgcatc gttttggaat tacccatcat cgcaaccgca
     6301 gaagtaaagg cgcagtaaag agcgaattac tggagataga tggaatcgga gaaaaaacaa
     6361 tgcaggatct tattcgtaag ttcaaatcaa taaaacgcct gaaagaagct gcagaaaacg
     6421 aaattgcaga agttgttggg cctgcaaagg cgaagatact tgttgcgtac tttactgata
     6481 acaaggaata gaagccattc aaaggggaat tctaccgtct ttccggtttt attcatcagg
     6541 aattactttt tcacgtattt tgtttaaaga ttcatctgct atgaaaaagt tatttttctt
     6601 t
//
LOCUS       DAOMYG010000142         6586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3978_length_6586_cov_8.692237, whole genome shotgun sequence.
ACCESSION   DAOMYG010000142 DAOMYG010000000
VERSION     DAOMYG010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6586
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3978_length_6586_cov_8.692237"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..2806)
                     /locus_tag="PK610_05955"
     CDS             complement(194..2806)
                     /locus_tag="PK610_05955"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HRJ35509.1"
                     /translation="MKRIPLVLLLLKALLSSAQSPCTGNFHLQAAMQNPESSNQLFHL
                     AAEGDRSVLEDYLSKHGGRIKYSTGSGYFIVLKGSDLPGFCALPGIKKVVYDASQPTI
                     LNDTMRVRNNIDSIHQGFSPLVSSFSGRGVLVGIIDTGVDFLHPDFLDSNGRTRILAI
                     WDQSAGFDPVLTPHYGYGRVWDSTQIMAGQCTQVDASAHGSTVAGTTSGNAGATGHHK
                     GIAPESNLLVVKTNFSANNWLQTIADATDWMYHIGDSLGMPVVINASVGTYSGSHDGR
                     DPAARFIDSLIRAKRGRLFVCAAGNSGNSSVYGNYHLRQQPGNDTTFSWFQYNASSAL
                     GYGAVYFEAYADTADLNLMQFSIGADAHTPVYSYRGRTPFRSVVDVGTGILTDTLWNG
                     PNKLAVVDFSLERIHDVYKLSVHLKQPDSSQYLFRFISTGNGTLDVWSASWMGISGMV
                     QPAALPSVGMYPEMARYTAPDSAQVIVSSFSCLPTVITTANYVGSNAYVDYNGTLQTF
                     PSVVNTISVNSSRGPTRDNRMKPEIAATGDVTMSAGPLASMAWMRINEPHKVSQDSMH
                     IRNGGTSMAAPVVAGIGALLLEKCPNLRWDEFMDLITQTAKSDAQTSGTPNITWGYGK
                     IDGFRALSASNYSPQWSGNTVFCNGDSSYIQAGNYTEYQWSNGNNNQGQWFFQSDTVS
                     LQVVDERGCFSREEEILLAETASLPTPVFTISLPLPPEFILETTLTGYDLWWTVVNGT
                     DTIYSGNANPVQITSDITLYICATLTITDSNGCSSTSQVECESWWSVEENDMQNAFVL
                     WPNPAGHSFNLEMLSSQQVHSWMIFSLDGKLIRQGAITTNTQNIVIDLPSGIYSVGVV
                     IEGGTVWKKLVVIN"
     gene            2946..3518
                     /locus_tag="PK610_05960"
     CDS             2946..3518
                     /locus_tag="PK610_05960"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HRJ35510.1"
                     /translation="MDNAKNRMLDIFDIYEKMERNNIMLSFKGTVTSELLTSILQIME
                     SRLDTTDDSPKIKKKVFNVLVECLQNLYHHIDEVDELDGPFSSKSAIFMMAKNGDDYI
                     IMTGNFILNQNVNGLRDKLDAINAMDREQLKEYYKSVLNNGTMSAKGGGGLGMIDIAR
                     KSGQKLEYAFQPVDSDNSFFSLNVKVTPNV"
     gene            3524..3904
                     /locus_tag="PK610_05965"
     CDS             3524..3904
                     /locus_tag="PK610_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HRJ35511.1"
                     /translation="MENIQIDGTPKTPTVTFSKDNGVLEIRGRSIPENSIEFYKPLID
                     WIEAYGKSPKGDTMVNIKLEYFNTSSSKCILDVFKKLETVSGTNMTINWYYEEDDEDM
                     LEAGEDYQAIINIPFKMIEVAELD"
     gene            complement(3947..4363)
                     /locus_tag="PK610_05970"
     CDS             complement(3947..4363)
                     /locus_tag="PK610_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35512.1"
                     /translation="MNADEKQGISSELIQLPFATMKFREEGILEIRINPYHITTSDDL
                     RFLHKLKGGKGGGKKYPNLIVMGFHSSADHTARAFSASEESQAYRKADAFVISSLAQR
                     IIANFYIKVQRPKVPTRFFNNEELALNWLRTFLPKK"
     gene            4625..5677
                     /locus_tag="PK610_05975"
     CDS             4625..5677
                     /locus_tag="PK610_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ35513.1"
                     /translation="MKNSLLLVALLLGLSASSQTLNSPESVEYNDASGEWFISNRAAH
                     TILRRSPSGTLSTFASGLTTGPHGMEIVGNRMFVCDGGTLREYNINTGANVQNVNLGA
                     SFLNGITHDNNGNLYITDFTAKNIYRFNMNTGAYNIFVGSLVKSPNGIIFDEDDDRLV
                     FVNWGSSAPIMAIAMADSSLSTLTTTSLGNIDGIAMDCQGNFYVASWSPNRITMFDNS
                     FTGAGTNMGYTGLSSPADIYFAQATDSLGVPNSGNNTVTFYSITSCLAPNAIEEQTPA
                     SMLYFFPSSDGQIRLEFSGFDQNKSVEIMDISGKQVFVQQTQSTSINTQLPEGIFIVR
                     VNDGIRSVTKKIVNLR"
     gene            5710..>6586
                     /locus_tag="PK610_05980"
     CDS             5710..>6586
                     /locus_tag="PK610_05980"
                     /inference="COORDINATES: protein motif:HMM:NF016301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HRJ35514.1"
                     /translation="MTLLFFFVVNSFLSFRMIQLGKEYKMEMSEYTDILSFKERLLNG
                     KEWLLGSWEANARLEKAQSHIDNALFALEMQNWYSSAFIISALLFLFIGYLAFIKDAK
                     APRIRAFTLISVAALCLLNGIFMPMLEIGAYNLDLQIPIVFEDPFFHNKLDMSKEFSG
                     HMYYYYQNKSVVDLIGMLFAQKNFVVGVAILSFSILLPVLKLSLSLLMLANRNIRHNT
                     GIKWFVSKIGKWSMADVFVAAAFLSYLSFNNMNSGIETEAHSLPGLYYFLAYCILSIA
                     SSLFIEKAIHLEEAAY"
BASE COUNT         1846 a         1438 c         1426 g         1876 t
ORIGIN      
        1 gaagatctgc gaaagtacga tggtgtcgag gtgaccggac tggtggagtc ggtaggagtt
       61 aagaacatcg ttttaacatt ttttatcgtt ttcgagttct atgtattgag ttggtatttt
      121 aatctgatct atcgttacat ttgatttctg ctaattatca ttttagaaaa aaagccgctt
      181 ttgggcggct tttttaattt ataactacca gctttttcca aaccgttcct ccttcaatca
      241 ctactcctac cgaatagatt cccgaaggca gatcgatgac aatgttttgt gtgttggtag
      301 tgattgctcc ctgacggata agtttaccat cgagtgaaaa gatcatccac gaatgaactt
      361 gctgagagga tagcatttcg agattgaaag agtgtccggc aggatttggc cagagcacaa
      421 atgcgttttg catatcgttt tcttccacac tccaccacga ttcgcattcc acctgcgacg
      481 ttgaactgca gccattggaa tcggtgatgg ttaaggttgc acaaatgtac aaggtgatat
      541 cggaagtgat ctgcaccgga ttggcatttc cgctgtaaat cgtatcggta ccgttaacga
      601 ctgtccacca caaatcgtaa cccgttagcg tggtctccag aataaattca ggtggtaatg
      661 gcaatgaaat ggtaaaaaca ggtgttggaa gacttgctgt ttctgcaagc aaaatctctt
      721 cttcacgact aaaacacccc cgttcatcaa ctacctgtag cgagacagta tcactttgaa
      781 aaaaccactg cccctggttg ttattgccgt tgctccattg gtattcggtg taattgcctg
      841 cttgaatata cgatgaatct ccattgcaaa acacagtatt tcccgaccac tgcggcgagt
      901 aattactggc ggacaatgca cggaatccgt caatttttcc atagccccag gtgatattgg
      961 gcgtgcctga agtttgcgca tcactttttg ccgtttgggt gatcaggtcc atgaattcat
     1021 cccaacgtaa attcggacat ttctccaata gcaatgcacc aatccctgcc acaaccggcg
     1081 ctgccatgga agtcccccca ttacggatat gcatgctgtc ctgcgaaact ttatgcggtt
     1141 cattgatgcg catccaggcc attgaagcaa gcggaccggc actcatcgtt acatctcctg
     1201 tggcggcaat ttcgggtttc atgcggttat cgcgtgtagg accacgactg gaattcaccg
     1261 agatcgtatt caccactgag gggaaggttt gaagtgttcc gttataatcc acataagcat
     1321 tagatcctac ataattcgct gtagtaatta cagtgggtaa gcaggaaaag gaagaaacaa
     1381 tcacctgtgc agaatcaggt gcggtatagc gtgccatttc agggtacatg ccaacagaag
     1441 gcaatgcggc aggctgtacc attcccgaaa tgcccatcca ggaagcagac catacatcca
     1501 gcgttccgtt acctgtgctg atgaatcgaa aaagatattg ggaagaatcg ggttgtttca
     1561 gatggaccga caacttgtat acatcgtgaa tgcgctcaag tgaaaaatcc acaaccgcga
     1621 gtttgttcgg accattccac aaggtatccg taagaatgcc tgttcccaca tccaccacac
     1681 tgcgaaaagg cgtcctgccc cgataagaat aaaccggtgt gtgtgcatct gccccaatgg
     1741 agaattgcat cagattcaga tcggctgtgt cggcatatgc ttcaaaatac acggctccgt
     1801 atcctagagc cgatgatgca ttgtattgaa accaggaaaa ggtggtatcg ttaccgggtt
     1861 gctgacgcaa atggtaattt ccgtaaacac tgctgttacc tgaattccca gcagcacaca
     1921 caaagagtcg tcctcttttg gcccgaatca ggctatcaat aaaccgcgca gcaggatccc
     1981 gtccgtcgtg cgatccgctg taggtaccca cgctggcatt gatcaccacg ggcataccta
     2041 aggaatcgcc aatatgatac atccaatcgg tggcatcggc aatggtctgc aaccagttgt
     2101 tggccgagaa attggttttt acgaccagca gattactctc cggagctatt cctttatgat
     2161 gacctgtggc tcctgcgttt ccggacgtgg ttccggccac agttgaaccg tgtgcgctgg
     2221 catcgacctg cgtacattga ccagccatga tttgggtgga atcccatacc ctgccgtaac
     2281 cgtaatgggg tgtcagtacc gggtcaaaac cagccgactg atcccaaatg gcgagaatac
     2341 gtgttcggcc attgctatcg agaaaatcgg gatgaaggaa atctacacct gtatcaatga
     2401 tgcccaccaa aacacctctt cccgaaaaag atgaaaccag cggagaaaag ccctgatgga
     2461 tggaatcgat attgttgcgc acccgcatgg tatcatttag aatagtaggc tgtgaagcat
     2521 cgtaaacaac cttttttatt ccgggtaaag cacaaaagcc aggtaaatca ctccccttta
     2581 aaacaataaa ataaccggaa ccggtgctgt atttaattcg gcctccatgt ttggataaat
     2641 agtcctccaa aaccgatcta tctccttcag cagcaagatg aaagagctgg ttggacgact
     2701 ccgggttctg cattgctgcc tgcaggtgga aattaccggt acagggcgat tgtgcagaag
     2761 acagcagcgc tttaagcaat agcaggacaa gcgggattcg tttcatcagt ggatataaag
     2821 ttacaaaagt cggaacagaa cttgagggcc ggcccctcat ttcgttcgta gaagcttttt
     2881 ccaccatctt ttgggtatat ctaaaaaaat ccctttagtt tgtaatatct aaatcttaga
     2941 tatatttgga caacgcaaaa aacaggatgc tggatatttt tgatatctac gaaaagatgg
     3001 aacgcaacaa catcatgctc tcattcaagg gcacggtgac gtctgagttg ctgacctcta
     3061 ttctacaaat tatggaatcg cgtctggata ccaccgacga ttcgcctaag atcaagaaaa
     3121 aggtcttcaa tgtacttgta gaatgccttc agaaccttta ccatcacatc gatgaagtgg
     3181 atgaactgga tggtccgttc agttcgaaat ctgccatatt tatgatggca aaaaatggtg
     3241 acgattacat catcatgact ggtaatttca ttctcaacca gaatgtaaat ggtttgcgtg
     3301 ataaactcga tgccatcaat gcgatggatc gtgaacaatt gaaagagtat tacaaatccg
     3361 ttttaaacaa cggaacgatg tcggcaaaag gcggaggtgg cttagggatg attgatattg
     3421 cacgtaaatc gggtcaaaag cttgagtatg cttttcagcc ggttgacagc gacaattcct
     3481 ttttctcatt aaatgttaaa gtaacaccca acgtataaac aatatggaaa acattcaaat
     3541 tgacggcaca ccaaaaacgc ctactgttac cttcagtaag gacaatggag tacttgaaat
     3601 ccgcggccgc tcgattcctg agaattcaat tgaattctac aaaccactta ttgattggat
     3661 cgaagcatac ggtaagtctc caaaaggcga taccatggta aatatcaagc tggagtattt
     3721 caatacttct tcttccaaat gtattcttga tgtgttcaaa aaactcgaaa ctgtttcggg
     3781 taccaatatg accatcaatt ggtattacga agaagatgat gaagacatgc tcgaagccgg
     3841 tgaggactac caggctatca tcaacattcc gtttaaaatg atcgaagtgg ctgaacttga
     3901 ttaagcctca tcagtaatat ttagaaggga gaccaatggt ctccctttat ttttttggta
     3961 aaaatgtccg aagccagttt aaagccagtt cttcattgtt aaagaagcgt gtcggcacct
     4021 taggtcgctg tacctttatg taaaagttgg caatgatccg ctgggccagt gaactgatca
     4081 caaaagcatc agctttcctg taagcctgac tttcttccga ggcactgaat gctcttgctg
     4141 tatgatcggc actggaatga aaacccatca cgatcaaatt ggggtatttt ttccctccgc
     4201 cttttccccc ttttaacttg tgaagaaatc gtaaatcatc gcttgtggta atatggtaag
     4261 gattgatacg gatttcaagt ataccctcct cccgaaattt catggtggca aatggtaatt
     4321 gaatcaattc agaacttatt ccctgctttt catccgcatt cattccttgt ttgattttgc
     4381 attaaagtat acaaaatatt ctatcaggga ccatattccg aaaaaaatca gccgtacagt
     4441 taatagatcg gtactgccca aagctttagc ggtaaaatgc agggcagaat agatcagaac
     4501 tggataggta atcctgaaga ggaacttttg agcttttggt agttcacgta atgttttgat
     4561 tagcttcacg cctccaaaat acaagcatac aactgaattt cctatcttga ggaaaattcg
     4621 atccatgaaa aacagcttac tgcttgtcgc tcttctgctt gggctttctg cttcatcaca
     4681 aacccttaat tcacccgaaa gtgtggagta caacgatgcc tcaggggaat ggttcatttc
     4741 caatcgcgca gctcatacca ttttgcgtcg ttctccttca ggtacattat caacctttgc
     4801 cagtggtcta acaacaggtc cgcacggaat ggaaatagtg ggtaaccgca tgtttgtttg
     4861 cgatggtgga accttacgtg aatacaatat caatacaggg gctaatgtgc agaatgtaaa
     4921 tctaggagcc tcgtttctca atggaatcac gcacgacaac aatggaaatc tttacatcac
     4981 tgattttact gcgaaaaata tttatcgttt caatatgaat accggagcct acaacatatt
     5041 tgtgggatca ctggtaaaat ctcccaacgg aattattttt gatgaagacg atgatcgtct
     5101 cgtttttgtg aactggggat caagcgcacc cattatggca attgcaatgg ccgattcttc
     5161 gctcagtaca cttaccacaa cttcattggg gaatattgac ggaattgcga tggattgtca
     5221 gggcaatttt tatgttgctt cgtggagtcc gaaccgcatt accatgtttg ataattcatt
     5281 taccggagct ggaaccaata tggggtatac aggattatca agtcctgccg atatttattt
     5341 tgcacaggct accgattcgc tcggtgtacc taattcagga aacaacacag ttaccttcta
     5401 ctctattact tcttgccttg cacccaacgc aatcgaagaa cagactcccg cttcaatgct
     5461 gtatttcttt ccaagttccg acggacaaat ccgcctggag tttagcggtt ttgatcagaa
     5521 taaatccgta gagatcatgg atatttccgg gaagcaggtt tttgttcaac aaacgcaatc
     5581 tacctcaata aatacgcagc tacctgaagg tatttttatt gttagggtaa acgatggtat
     5641 ccgttctgta actaagaaaa tagttaacct ccgatgaagg aaaagcgatc gctgattcaa
     5701 ctgttttggt tgaccctcct gtttttcttt gtggtcaatt catttctttc tttccgcatg
     5761 attcaattgg ggaaagaata caaaatggaa atgtcggaat acaccgatat actcagtttt
     5821 aaggagcgat tactaaacgg aaaagaatgg ttgctgggaa gctgggaagc caacgcacgg
     5881 ctggaaaaag cacaatcgca tattgataat gccttgtttg cactcgaaat gcagaattgg
     5941 tattcttctg cattcatcat ttcagcattg ctctttttgt ttatcggtta cctggctttt
     6001 atcaaggatg ctaaggctcc gcgtattcgc gcatttactt tgatttcggt agcagcattg
     6061 tgcctcctta acggaatttt tatgcccatg ctcgagattg gcgcctataa tctcgatttg
     6121 caaattccaa ttgtgtttga agatcctttt tttcataata aactcgatat gagcaaagag
     6181 tttagtggtc atatgtatta ttattaccaa aataaatcgg ttgtagatct gatcggaatg
     6241 ctctttgccc aaaagaattt tgtggttgga gttgcaattc tttcgtttag tattctgtta
     6301 ccggttttaa aactgagttt gtcgctactg atgctcgcta accggaacat tcgtcataac
     6361 accggtatta aatggtttgt aagtaaaatc gggaaatgga gcatggcgga tgtatttgtt
     6421 gcagctgcat ttttatcgta tttatcgttc aataacatga actccggcat tgaaacagaa
     6481 gcgcattctt tacccggact gtattatttt ctggcgtatt gcattttgtc catcgcctct
     6541 tcccttttca tcgaaaaagc catacacctc gaagaagctg cttacc
//
LOCUS       DAOMYG010000143         6578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_3988_length_6578_cov_10.768052, whole genome shotgun sequence.
ACCESSION   DAOMYG010000143 DAOMYG010000000
VERSION     DAOMYG010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3988_length_6578_cov_10.768052"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..499
                     /locus_tag="PK610_05985"
     CDS             224..499
                     /locus_tag="PK610_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35515.1"
                     /translation="MTAKITLMTFPLFRKYPNNASFFRLDSAEEFLELKIMGSRYSLI
                     SIKAKILPDRNYIADMIAMENDHWIASSEEEFERALNECRQNRKELL"
     gene            complement(491..1756)
                     /locus_tag="PK610_05990"
     CDS             complement(491..1756)
                     /locus_tag="PK610_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35516.1"
                     /translation="MIAIREPRSVFGSPSGRVLFLLCASLVFCLLVTRKAFIPADVDY
                     PRVPMLPFFDPELPLLLLSLLFGLLMFSLGILPFLKDRKKVVVFIALLFLVFFLDDID
                     RFQPWFFIHFPMIVFVALRNENGFQFARWGMIFMYFWGGLHKLNQNFAWEVFPYLMKP
                     FKMTSIHYLPPEEIGSYSLPSAFHLAWIVPAVELLIALFLLLPVLRKAGVFLAIGLHL
                     ITLYILGPFGHNWNSVVWIWNTELLLLVVVLFYRDSTLLQEQFAFFRKGKLAVAFLVL
                     MGIAPVLWYFNTWPHALSYHLYSGYNPQVNFWFGKKELSGRNSFMRPPDEGVIENNPK
                     IVGTPWEDFTFYDVERRESFIMVDHHAMKEMNVPIFAEEFYFRLLGKKLCKCFGYSGN
                     DAGIRMELRTKFKAHLVERSLSCRELLLK"
     gene            complement(1779..2831)
                     /locus_tag="PK610_05995"
     CDS             complement(1779..2831)
                     /locus_tag="PK610_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRJ35517.1"
                     /translation="MMRALFLFAGILSAVLSVRAQDIHFTQVLNNPLHINPANAGGYD
                     GYERVVLNYRNQWSAANATYSTMGFSFDMPFFQGKRSDKAHLGAGLTFFSDRAGDSRF
                     GITQGALSLAGIVPFDKNNRLIAGLQIGYGQRSADISKLQFGTQFNGQEHDPNLFSGE
                     SNTMNSFGYMDMGAGLRYEYVNKNDHIQGWDFSRVSIGAAMYHINQPEMHYLAGSSEK
                     INRKLVVHGSAHFDLPNSTFSLIPFFAYFSQGPLSELNMGTLLRIELNPGTKITGLLT
                     ESGLLAGLQWRRMDAFIPYVGYEFSGFSVGFTYDHNYSTLKTVSRGIGAFEISLKYHN
                     VKGALFTRRTGSRMYD"
     gene            complement(2828..5281)
                     /locus_tag="PK610_06000"
     CDS             complement(2828..5281)
                     /locus_tag="PK610_06000"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRJ35518.1"
                     /translation="MRVLLAVFFLVCAPFSFAQVVINEYSCANVNGFTDNYGEREDWV
                     EFYNPSGAAIDLAGFHLSDRLNNPTKWTFPSGSNVPAGGFLRVVCSGRNEVSGGFIHT
                     GLKLTQTKPEVLVFAAPGGSVLEAFPLNPTLSNHSRGRTPDGGATWTLFQTPTPGASN
                     GAASPEYSARPLMGMPAGMYSGTISVSITTTDPTATIRYTVNGSEPTAASPAYSGPIS
                     ISTTTVLRARGFSSTPGVPSSFIESNTYFINVSHSIPVLSLFGDDVGVLLGGTQIDPI
                     ANVEYFTSTGVLLAEANGEANKHGNDSWAYNQRGIDFIARDQFGYSYALKNTLFSRKS
                     RDEYQKIIIKCGANDNYPFEDGAYIRDAYLHTLSHDGNLHLDERTYEPCVMYVNGQYW
                     GLYEIREKVDDHDFTSHYYDQDEFNVQFLKTWGATWSEYGGPQAQTDWNALRAFINAN
                     DMSIQANFDYVDSLYNWKSLCDYFILNSYAVCMDWLNWNTAWWRGMDPNGDKKKWRYV
                     LWDLDASFGHYINYTGIPNTGPDADPCDPEGLPNPGGQGHTEILNKLMDNPGFNAWYV
                     NRWIELTNTTFSCTNMIAKLDSLINRISPEMPAQIARWGGTLTAWQGHVQEMRDFINT
                     RCANIDDGLVDCYNLSGPYQVTIDVDPPGTGMVYLNDLLIPTYPYTTTLYGGIPNQLT
                     AVPNPNYVFVDWTMSANTPLPNTTDSIITIDFVANDLVVAHFKSPVAVFIPTGFSPNG
                     DGVNDVLQIMGEGILSLNLAIYDRWGQRVFQTTDVNTFWDGTFNGQPLNSGVFAYKVF
                     AVLADGTEISQSGNITLMR"
     gene            complement(5436..5915)
                     /gene="rplQ"
                     /locus_tag="PK610_06005"
     CDS             complement(5436..5915)
                     /gene="rplQ"
                     /locus_tag="PK610_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRJ35519.1"
                     /translation="MRHGKTVNHLGRQHGHRRALMANMACSLVQHKRINTTVAKAKAL
                     RKYIEPLITKSKNDTTHNRRTVFSYLQSKDAVTELFRVVSPKVADRPGGYTRIIRTGF
                     RLGDSAEMCMIEIVDFNELLLDTKGDKKAKTTRRGRTSKAKKAEGAAPAAEEGASEE"
     gene            complement(5955..>6578)
                     /gene="rpoA"
                     /locus_tag="PK610_06010"
     CDS             complement(5955..>6578)
                     /gene="rpoA"
                     /locus_tag="PK610_06010"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRJ35520.1"
                     /translation="VLNPDLVICTMDKNVNLNMEITVAKGRGYVSAEENKASNAPVGT
                     IAMDAIFTPIRNVRYNIENYRVEQKTDYEKLVIEVSSDGSIHPKEALKEAAKILIQHF
                     MLFSDEKITLDTIEKQETEEFDEESLHMRQLLKTKLVDMDLSVRALNCLKAADVETLG
                     ELVAYNKNDLLKFRNFGKKSLTELEDLVHAKGLHFGMNVAKYKLDKD"
BASE COUNT         1980 a         1531 c         1337 g         1730 t
ORIGIN      
        1 aatgatccct tgctgtcatt ttttcaaaat tacattgttt tagttaaaac accctattct
       61 tcctgtttgt tcccaaagca aactatcccc tgaaacagaa atcacccctg aagcagaaat
      121 ctccttgcag tcgatttcgc ttcagaatcg atttccctca gggattgggg ctacctaatc
      181 gggaatcggg caattggaaa tcggtggtta aatcaaacta tctttgacag cgaaaatcac
      241 actcatgacc ttccctctct tccgcaaata ccccaataac gcgtccttct tccggcttga
      301 ttcggcagag gaatttctgg aattgaaaat tatggggagt cgctattcgc tcatttctat
      361 taaagcaaaa atattgcccg accgcaatta catagccgat atgatcgcca tggaaaacga
      421 tcactggatt gcttcttcgg aggaggaatt tgaacgagcg ctgaacgagt gccggcagaa
      481 ccgcaaagaa ttactttaac aaaagctcgc gacagcttaa cgaacgctca accaaatgtg
      541 ctttaaattt tgtccgcaat tccatgcgta ttcctgcatc gttgcccgaa tacccaaaac
      601 acttgcagag tttcttacct aataaacgga aataaaattc ctcggcaaaa atggggacat
      661 tcatctcttt catggcatga tgatcgacca taataaaact ttcccttcgc tccacatcat
      721 aaaaagtaaa atcttcccaa ggcgttccta caattttcgg attgttttcg atcacacctt
      781 catcaggcgg acgcataaag gaatttcttc cgctaagctc cttttttcca aaccagaaat
      841 taacctgcgg attatagccg ctgtacaaat gatacgacaa ggcatgcggc catgtattaa
      901 aataccacaa aactggggca atgcccataa gtaccaaaaa agcaacggct aattttccct
      961 tacggaaaaa tgcaaattgc tcctgaagta aagttgagtc gcggtaaaac aatactacta
     1021 caagcagcaa taattcggtg ttccaaatcc acacaacaga gttccagttg tgaccgaatg
     1081 gacccagaat ataaagcgta atcagatgca aacctatcgc caaaaaaaca ccggcctttc
     1141 tgagaacagg tagcaataaa aaaagtgcta tcagcaattc cacagccggc actatccaag
     1201 caagatgaaa agcagaaggc aaactgtacg aaccaatttc ctcgggagga agataatgaa
     1261 tggaggtcat tttaaaaggc ttcatcagat aaggaaacac ttcccaggca aaattctgat
     1321 tcaacttatg caaaccaccc cagaaataca taaatatcat tccccagcgc gcaaactgaa
     1381 atccgttttc attgcgcaat gcaacaaaaa cgatcatcgg aaaatgaatg aaaaaccagg
     1441 gctgaaagcg atcgatatca tccagaaaaa aaacaagaaa aagcagcgca atgaacacca
     1501 caactttttt cctgtcctta agaaagggca aaatacctaa ggaaaacata agtaatccga
     1561 acaataagga aagcaaaagc aagggtaatt ccggatcaaa aaacggaagc atgggaacac
     1621 gtgggtaatc cacatcagcc ggaataaatg ctttgcgggt gacaagcaaa caaaaaacaa
     1681 gcgaggcgca aagcaaaaaa agcacccttc ctgaaggact accaaaaact gaacggggtt
     1741 cgcggattgc aatcataccg caaaataaag cgaaagaatc aatcgtacat cctgctgcca
     1801 gtgcgacgtg tgaacaatgc acctttaaca ttatggtatt tcaacgaaat ctcgaatgca
     1861 ccaattccgc gcgatactgt tttcaaggtt gaataattgt gatcgtaggt aaatccaact
     1921 gaaaaaccgg aaaattcata tcccacgtag ggaataaagg catccattct cctccactgc
     1981 aatccggcaa gcaagcccga ttcggtaagc aatcctgtaa tctttgttcc gggatttaac
     2041 tcaatgcgca acagcgttcc catgttaagc tcggataaag gaccctgcga aaaatacgcg
     2101 aaaaagggga tcagcgagaa tgtggaattc ggtaaatcaa aatgcgccga accatgtacc
     2161 accaacttac gattgatttt ctcactgcta ccggccagat aatgcatttc gggctgattg
     2221 atatgataca ttgctgcacc gatgcttaca cgactgaaat cccagccctg aatatgatcg
     2281 ttcttattga catattcgta acgtaaacct gcacccatat ccatgtatcc aaatgaattc
     2341 atggtattgg attctccgga aaataaattc ggatcatgct cctgaccatt gaactgcgtt
     2401 ccgaattgca gcttggaaat atccgcagaa cgttgtccgt aaccgatctg caatcctgca
     2461 atcaaacgat tgttcttatc gaacggaaca atacctgcaa gcgatagtgc tccctgtgta
     2521 attccaaatc gtgaatctcc tgcacggtcg ctgaaaaaag ttaatcctgc gccaaggtgt
     2581 gccttatccg aacgcttccc ctggaagaaa ggcatgtcaa atgaaaatcc catggtagaa
     2641 tacgttgcat tggcagccga ccactgattt ctataattca ataccacacg ctcgtaccca
     2701 tcgtaaccac ccgcattggc aggattgatg tgcaagggat tattcaacac ctgcgtgaag
     2761 tgaatatcct gcgcgcgaac agaaagaact gcgcttaaaa ttcctgcaaa aagaaagagt
     2821 gctctcatca tcgcatcagt gttatgtttc cactctgact aatttctgtt ccgtctgcaa
     2881 gaaccgcgaa aactttatag gcaaacacac ccgaattaag cggctgacca ttaaacgtac
     2941 cgtcccagaa agtatttaca tccgttgtct gaaaaacacg ttgtccccag cgatcataaa
     3001 tagcaaggtt gagcgaaagt attccctctc ccataatctg caacacatcg ttgacgccgt
     3061 ctccgtttgg tgaaaatcct gtgggaataa ataccgcaac aggcgattta aaatgtgcca
     3121 ccaccaaatc attggcaaca aaatcaatag taataattga atctgttgta ttgggtaaag
     3181 gcgtatttgc actcatcgtc caatcgacaa acacatagtt tggattggga acagcggtaa
     3241 gttgattcgg aattcctcca tacaatgttg tggtgtaggg atacgtcgga atcaataaat
     3301 cattcagata aaccattcct gttccgggag gatctacatc aatagttacc tgataaggac
     3361 cggagagatt gtaacaatca accagaccat catcaatgtt agcacaccgc gtattgatga
     3421 aatcacgcat ctcctgaaca tgtccttgcc atgccgtaag tgttcctccc caacgcgcaa
     3481 tctgagcggg catttcaggt gatatacgat tgattaagct atccagcttg gcaatcatat
     3541 tcgtacagga gaaagtcgta tttgtaagtt cgatccaacg gtttacatac catgcattga
     3601 aacctggatt atccatcagc ttgttcaata tttcagtgtg accttgcccg ccgggattgg
     3661 gtaagccttc cggatcacaa gggtcagcat caggacctgt attggggatt cccgtataat
     3721 ttatatagtg accgaaggat gcgtccaggt cccacaaaac atatctccac tttttcttat
     3781 cgccattagg atccattcct ctccaccagg ccgtattcca attgagccag tccatacaaa
     3841 cggcatagga attcagaata aaataatcgc ataaactttt ccagttatac agcgaatcaa
     3901 cataatcgaa attcgcctga atgctcatat cgtttgcgtt gataaatgca cgtaaagcat
     3961 tccagtcggt ttgcgcttgc ggaccaccgt attcagacca ggttgctccc caggttttca
     4021 gaaactgtac attgaattca tcctgatcgt aataatggct ggtgaaatca tgatcatcca
     4081 ctttttcacg aatctcatac aatccccagt attgaccgtt cacgtacatt acacatggct
     4141 cgtaagtacg ttcgtcgaga tgtaaattac catcgtgtga tagtgtatgc aaatacgcat
     4201 cacgtatgta agcgccatct tcaaatgggt aattatcgtt ggcaccacac ttaataatta
     4261 ttttctggta ttcatcgcgc gatttgcgac tgaacagggt attctttaat gcataactgt
     4321 agccaaactg atcgcgggca atgaaatcaa ttccccgctg attataggcc caggaatcat
     4381 taccatgctt atttgcttct ccattggctt ccgcaagtaa aacaccagta gaagtgaaat
     4441 attcaacatt tgctatggga tctatttgag tgccacccaa taaaacgcca acatcatctc
     4501 caaacaatga cagaacggga attgaatgcg aaacgttgat gaaataggta ttactctcaa
     4561 taaacgaaga gggcacaccg ggtgtggaac tgaatccacg tgcacgcaaa accgttgttg
     4621 tggatattga gatcgggcct gaataggcag gcgatgctgc agtgggttca gaaccgttta
     4681 cggtatagcg aatggttgcg gttggatctg tggttgtaat ggaaaccgaa atggttccgc
     4741 tgtacattcc ggcaggcatc cccataagcg gtcgtgctga atattccggc gatgcggcac
     4801 cgttacttgc cccgggtgta ggagtttgaa aaagcgtcca tgttgctccg ccatccggag
     4861 ttctacctct actatgatta cttaatgttg gatttaatgg gaaagcctca agaacagatc
     4921 ctccgggtgc ggcaaaaaca agaacttccg gttttgtttg tgtgagctta aggccggtgt
     4981 gaataaatcc tcccgatact tcatttcttc ctgaacacac tacacgcaaa aaccctcctg
     5041 caggaacatt ggatccggag gggaatgtcc atttggtagg attgttcaag cgatcgctca
     5101 aatgaaaacc tgcgagatca atcgcagcgc cggagggatt gtaaaattca acccaatctt
     5161 cccgctctcc gtaattatcg gtaaagccat ttacattggc gcaggaatat tcattaatca
     5221 caacttgtgc aaacgaaaat ggtgcacaaa caagaaaaaa tacggcaagc aaaaccctca
     5281 tgggtaataa gaattagtcc cctaaattta gtgaatattt acaattgatt aagcctttgg
     5341 ttcagccgat tgctcctttg aattggtttc ctaatgctga gcaaaaaaaa agtcccgata
     5401 cgaataccgg gactcttgaa tttaagatag ttagcttatt cttcagaagc accttcttct
     5461 gcagcaggag cagcaccttc agcttttttt gctttggatg tacgtccacg acgagttgtc
     5521 ttggccttct tgtcgccttt ggtatcgagc aacaactcgt tgaaatcaac gatttcgatc
     5581 atacacattt ctgcgctgtc acctaagcgg aatccggtgc ggatgatacg ggtgtaacca
     5641 cctggacgat ctgcaacttt aggagaaacc acgcgaaaga gttccgtaac agcgtccttg
     5701 ctttgcaggt agctgaatac agtacgacgg ttatgcgttg tgtcgttctt cgacttcgtg
     5761 attagtggct cgatgtactt gcgcaatgct tttgctttgg caacagtagt gttgatacgc
     5821 ttatgctgca ccaatgagca agccatattc gccataagcg ctcttctgtg cccatgctgc
     5881 ctgccgaggt ggttgactgt tttaccgtgt ctcatttttc ttcagatatt tctttccctt
     5941 tcaggagtgt tatactagtc tttgtcgagt ttgtacttcg caacattcat tccgaaatga
     6001 agtccctttg catgcacaag atcctcaagc tctgtaagtg atttcttacc gaagttgcgg
     6061 aacttcagaa ggtcgttctt attgtatgct acaagttcac caagtgtttc aacatcggca
     6121 gccttgaggc agttcaatgc acgaacagaa aggtccatat caacaagctt cgtcttaagc
     6181 agctgacgca tgtgcaatga ctcttcatca aattcttcag tctcctgctt ctcgatagta
     6241 tcgagagtga tcttctcatc agagaaaagc atgaaatgct gaataagaat ttttgctgct
     6301 tccttcaatg cttctttcgg gtgaattgaa ccgtcagaag aaacttcgat aacgagcttt
     6361 tcgtagtcag ttttctgttc aacacgatag ttttcgatgt tgtaacgtac attacggata
     6421 ggtgtgaaaa tggcatccat agcgatggtt ccaaccggag cattgcttgc cttgttttct
     6481 tcagcagaaa catatccgcg acctttggca acagtaatct ccatgttgag attaacgttc
     6541 ttgtccatag tgcagattac cagatcagga ttcaacac
//
LOCUS       DAOMYG010000144         6543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4019_length_6543_cov_9.049014, whole genome shotgun sequence.
ACCESSION   DAOMYG010000144 DAOMYG010000000
VERSION     DAOMYG010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4019_length_6543_cov_9.049014"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PK610_06015"
     CDS             complement(<1..787)
                     /locus_tag="PK610_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35521.1"
                     /translation="MKKMAVLFIMLFAIVSCSTENTGNSSAGNPEFDRLVKENEELKR
                     ELGGKDSAMNASIRILNEIEENLSVIRQKEGVINKSRNNDPELAVDQKDRIIEEIQVI
                     NSLMEDNRNKIASLRGKLKKGDMKIGELEKMIENLLTKIDDQEQTINSLREELAKYDI
                     AMDELTATLNETMAVVEEKEAELKTAYYVIGSTKELKENGILTKEGGFIGIGKIKKLR
                     EDFNKDYFTQIDISKTSEVILGGKKAEHRALPCWWQSSPRRTRP"
     gene            870..2648
                     /locus_tag="PK610_06020"
     CDS             870..2648
                     /locus_tag="PK610_06020"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRJ35522.1"
                     /translation="MRSFLICLGLLSILNGKSQSIDYTLRMGAPHTHYLEVEMVVSKL
                     SGKTLDVKMPVWAPGSYMVREFARNVDFFEATADGKKLPWKKTDKNTWSIATNGSKEI
                     RINYNVYAFEMSVRTSFLDDSHGYINGTSVFMYVKDKKNLSGKLTIEPHKNFKSISTG
                     LTYISNQNGKFQYSFPDYDFLADCPIEIGNQEIFSFNASGIEHEVAMYGEGNYDKERL
                     KQDMAKVVESCTKVFGENPNQKYVFIIHNLTVGSGGLEHLNSTTLQVNRWTYSGSAYT
                     DFLSLVAHEYFHLWNVKRIRADVLGPFDYDKENYSTLLWVLEGFTSYYDELLLLRSGF
                     HTQSEYLSVLSRIINTVENTPGAKIQSASESSFDAWIKGYRTNENSVNSQISYYTKGQ
                     LLGALLDIEIIQATNGSKRLDDLLSYLYQEFYKKQKRGFSDVEFRKAAEKIAGKELKI
                     FFEEYVEKTTAIDYEKYFAPLGMTLKKTSREKEIAFGARLTDSGGKLIVKEVTKGTSS
                     YESGLNVNDEIISCNGFRVDQTSFNKMIAASKIGDEIDLLISRDNIMQALKIKLLPNG
                     AWDHQVTVLQSTGENQEKLRMKWLGN"
     gene            complement(2659..2910)
                     /locus_tag="PK610_06025"
     CDS             complement(2659..2910)
                     /locus_tag="PK610_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35523.1"
                     /translation="MEFTKPGTYYSNKPTNKQQTLNTMKKQATLSRGIVMASAVVLLG
                     AMSLTSCSKGQHCPAYTGYKKYKKVATMPVVLKERKQIA"
     gene            2979..3572
                     /locus_tag="PK610_06030"
     CDS             2979..3572
                     /locus_tag="PK610_06030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ35524.1"
                     /translation="MIIGILHSSAGLSDEKLMEKYRSTGNTWFVGELYQRYSRPVSAM
                     CYSYFRNTEDAEDTAMEIFEVIMVDLKKHEIQNFKPWLHSVVRHYCLRKLDKSRKREA
                     NHQEFKKSGDPFMELSSELSLDSMNGEPSPDLIEELKKAIDSLKDDQRTCVELFFIQD
                     KSYQEIADITGYPLMKVKSHIQNGKRNLKIYLESLHG"
     gene            3565..4536
                     /locus_tag="PK610_06035"
     CDS             3565..4536
                     /locus_tag="PK610_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35525.1"
                     /translation="MANLNTYHSVPEDLKSFYEGISEDDLAAVDQIAMRVNKRVQSIA
                     GKAPLLYRSSFYIGSAIVVCGIALAVLFSYPSQESATSSLINTQKTILNNPGENNHSI
                     GGGIEEEDEKKEHVSSNEITKTGQLNKEEKSIQEGQQLNELEQTIETPLKNEEKQIEK
                     SQPIVDETRRMVEEENVLIFLRIAQVAVMGKTDLASLSGTSTRNTDVTNPNIGAKTST
                     KTSNNSYSLEDMPYHTGGDQGLISYISRNLNNKIHIRKKDAVYSVNVVFEVTAKGKVE
                     NVEILDDIPAPMADVIKQVILNDTGWNAGKKSGKKGALQLIVNISFV"
     gene            complement(4539..5966)
                     /locus_tag="PK610_06040"
     CDS             complement(4539..5966)
                     /locus_tag="PK610_06040"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ35526.1"
                     /translation="MNSSRTEHIAQLFRSSLRIEPTVDQQLFMDRFSHFLCGSERYRI
                     FILKGYAGTGKTTMMGELVRFLPMLRMNTELLAPTGRAAKVLSGHTGQRAYTIHKKIY
                     MLRNKAGYSSFELAHNPHSNTLFIIDEASMIGSGSSSNQDGFRSRDLLEDVLRYIYRG
                     KNCFALIVGDPAQLPPVGMADSPALSEEFIRDNFRLDVHSIELKQVVRQASDSGILYN
                     ATRLREQLSANANGFPKIETKGRPDVRQITGTELQDELEWAFGQYGAEGVRIITRSNK
                     RANLFNMQVRNRVFWIENELNGGDELMAVKNNYFWLPADSPAGFIANGDSLTVQKVKA
                     IREYHGLRFADVEVKLSDYPDLPEVEMRILIDTLQTEGPHLPAEKLKALFEAISEDYS
                     DEPDQKLRKEKIMNDPWYNALQVKFAWSVTCHKAQGGQWPVVFVDQGYLTEEMIDREY
                     LRWLYTAFTRASYRLYLINFSEKFF"
     gene            6069..>6543
                     /locus_tag="PK610_06045"
     CDS             6069..>6543
                     /locus_tag="PK610_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35527.1"
                     /translation="MNALKNIGYLAGKYFAQILVITLGLVVLMIGMKTDPDTGFKQNS
                     LFVLGGVALLLAGTISTLYVAEVINRMLHTIFLYVLLPIGVITYGYLDYRSIMDEVEY
                     RERVAMVNRETQQRLKDIRDAQVEFRAMYNRYAPSFDSLRWFIQEGRAMVINRLGD"
BASE COUNT         1940 a         1369 c         1359 g         1875 t
ORIGIN      
        1 gcgggcgcgt tcggcgcggt gacgattgcc accaacatgg cagggcgcgg tgttctgctt
       61 tttttccgcc taaaatcact tcactggttt tcgagatatc aatttgggtg aaataatcct
      121 tattaaagtc ttcacgtaac ttcttgatct ttccaatgcc aataaatcca ccttctttcg
      181 taaggatgcc attttccttt aattcttttg ttgaaccgat tacgtaatag gcggtcttta
      241 attcagcttc tttttcctct accactgcca tggtttcatt aagtgttgca gtgagctcgt
      301 ccattgcaat atcgtacttg gccagttctt cgcgaagcga gttaatggtt tgttcctgat
      361 cgtcgatttt ggtcaacagg ttttcaatca ttttttcgag ttcgccaatc ttcatatctc
      421 cctttttcaa ctttccacgg agactggcaa ttttattccg gttatcttcc attagtgagt
      481 tgatgacctg aatttcttct ataatgcggt ctttttgatc cacagccaat tcgggatcat
      541 tgttcctgct tttattgatt actccttctt tctgacggat taccgaaagg ttctcttcaa
      601 tttcattcag tattcggata gaagcattca ttgccgagtc cttccctcct aattcacgtt
      661 ttaactcttc attttctttc acaaggcggt caaactccgg atttccggca ctgctgtttc
      721 cggtgttctc ggtagaacag gaaacgattg caaaaagcat gataaaaagg acagccattt
      781 ttttcataaa gaagtgaatt tgagttcaaa caaaaataac aaaagttcga ttcggacagg
      841 gtgcttatat ttggggaaat acaataatca tgagatcatt ccttatttgt ctgggactat
      901 tgtccatttt gaacggaaaa tcacaatcca ttgattacac gctccgaatg ggggcgccgc
      961 atacccacta cttagaggtt gagatggtcg tttcaaaatt gtcgggtaaa accctggatg
     1021 taaaaatgcc ggtatgggct ccgggatcgt atatggtacg tgaatttgca cggaatgtgg
     1081 acttttttga agctactgca gatggcaaaa aactgccatg gaaaaagacc gataaaaaca
     1141 cctggagtat tgcaacaaac ggatcaaaag aaatcagaat caactacaat gtttacgcat
     1201 tcgaaatgtc ggttcgcact tcctttttag atgattcgca cggatatatc aatggcacaa
     1261 gtgtttttat gtatgtaaag gacaaaaaga atttgagtgg aaaattaact attgaacctc
     1321 ataaaaactt taaatccatc agcaccggat tgacttacat ttcgaatcaa aacgggaaat
     1381 tccaatacag ttttcctgat tatgactttc tggctgattg tccaattgag atcgggaatc
     1441 aggaaatctt ttcctttaat gcatccggaa ttgagcatga agtcgccatg tatggagaag
     1501 ggaactacga caaggaacgc ctcaaacaag acatggccaa agtggtggag tcctgcacaa
     1561 aggtgttcgg cgaaaacccc aatcagaaat acgtttttat tattcataat ctaaccgtgg
     1621 gctccggtgg attggaacat ctcaactcta ccacactgca ggtaaaccga tggacctatt
     1681 caggatcggc gtatacagat tttctgagtc ttgttgctca tgaatatttc catttgtgga
     1741 atgtaaaaag aatcagagca gatgtattgg ggccctttga ttacgacaag gaaaattaca
     1801 gcacactctt atgggtattg gaaggattta catcgtatta tgatgagttg cttcttttac
     1861 gttccggttt tcatacccag agcgaatacc tttctgttct gagcagaata ataaatactg
     1921 tagaaaacac tcccggtgcc aaaattcaat cagcctccga atcgagtttt gatgcatgga
     1981 taaagggata ccgtacaaac gaaaacagtg taaattccca aatttcctat tatactaaag
     2041 gtcagttgct gggcgcattg ctcgatattg agatcataca agctacaaac ggatcgaaac
     2101 gactcgatga tcttttgagt tatctctatc aggagtttta caaaaaacaa aaacggggat
     2161 tcagcgacgt ggaattcaga aaagctgccg aaaaaattgc gggtaaagaa ttgaaaatct
     2221 ttttcgaaga atacgttgaa aaaaccactg ctatcgacta cgaaaaatat tttgctccct
     2281 tgggcatgac gcttaaaaaa acctcgcgcg aaaaggaaat tgcatttggc gccagattga
     2341 ctgattccgg aggaaaatta atcgtgaaag aagttactaa aggaacatca tcctatgaat
     2401 cgggacttaa tgttaatgat gaaattattt cgtgcaacgg gttcagagta gatcaaacct
     2461 ccttcaataa aatgatcgct gcttcgaaaa tcggagatga aattgatttg cttatttcca
     2521 gagataacat tatgcaggca ctaaaaataa aactgctacc caatggcgcg tgggaccatc
     2581 aggtaacagt tcttcaatca actggtgaaa accaggaaaa gcttcgtatg aaatggctgg
     2641 gcaattaacc caactctatc aggctatttg ttttctttct ttgagaacaa caggcatggt
     2701 agctaccttt ttgtacttct tatatcctgt ataggccgga caatgttgtc ctttagagca
     2761 tgaagttaga gacattgctc ctaatagaac aacagcggat gccattacga ttccgcgaga
     2821 aagggtagct tgctttttca tggtgttgag ggtttgttgt ttgtttgttg gcttgttact
     2881 gtaataggta cccggttttg tgaattccat aaaattttgt ggaattttta taaaaaatgt
     2941 cctaaattgt agactatagc ctataaatca gatcattcgt gatcatcgga atcttacata
     3001 gcagtgcagg actcagcgat gaaaagctga tggagaagta tcggagtacc ggaaatacct
     3061 ggtttgtagg tgaattgtac caaaggtatt cgcgtccggt tagtgccatg tgttattcct
     3121 atttccgaaa taccgaagat gcggaagata ctgccatgga aatttttgag gttataatgg
     3181 tcgaccttaa aaaacatgaa atacagaatt tcaaaccctg gctccattcc gttgtaaggc
     3241 actattgcct gagaaagctt gataaatcaa gaaaaagaga ggcaaatcat caggaattca
     3301 aaaaaagcgg agatcctttt atggaattat cctccgaatt gtcccttgat tctatgaacg
     3361 gggaaccatc acccgacctc atcgaagaat tgaaaaaggc cattgatagc ttaaaagacg
     3421 atcaacgcac atgcgtagaa ctcttcttta ttcaggataa gtcctaccag gaaatcgctg
     3481 atatcaccgg ttatccttta atgaaagtaa aaagtcacat acaaaacgga aaacgcaatc
     3541 tgaaaatcta tctcgaaagt ctccatggct aatcttaata catatcattc tgttccggaa
     3601 gacctgaagt ccttttacga agggatttct gaagatgatc ttgccgctgt ggatcagatt
     3661 gccatgcggg taaataaacg tgtacaatcg atagcaggta aagctccttt attataccgc
     3721 agttcctttt atataggaag tgccattgtc gtttgtggta ttgctttggc agtgctgttc
     3781 tcctaccctt cgcaggaaag tgctacatca tcactaatca atacccaaaa aacgattctt
     3841 aataatccgg gtgaaaacaa ccattcaatt ggaggcggca tagaagaaga ggacgaaaag
     3901 aaagaacatg tatcgtctaa tgaaatcacc aaaacgggtc agcttaataa ggaagaaaag
     3961 tccattcagg aaggtcagca attgaacgaa ttggaacaaa ccattgagac tcctcttaaa
     4021 aacgaagaaa agcaaatcga gaaatcccag ccaattgttg atgaaacaag gagaatggtt
     4081 gaagaagaaa atgttctcat tttccttcgc attgcacaag ttgccgtgat ggggaaaaca
     4141 gatttagcct cactttcagg aacatcaacg cgaaacacag atgttactaa ccctaatatt
     4201 ggagctaaaa ccagcactaa aacttcgaac aacagctatt cgctcgagga catgccttac
     4261 cacaccggtg gcgaccaagg cttgatttct tatatctcca gaaacctcaa caataaaatc
     4321 catatccgta aaaaggatgc ggtttattcg gtcaacgtag tatttgaagt tacggcaaaa
     4381 ggcaaggtgg aaaatgtgga gatactggat gatattcctg ctccaatggc cgatgtcatt
     4441 aaacaggtaa ttctgaacga tacaggatgg aatgccggta aaaaatcagg taaaaaagga
     4501 gcgcttcaac tgatcgttaa catcagtttt gtctaagctc aaaagaattt ctcgctgaaa
     4561 ttgataagat aaagcctgta actggcgcgg gtaaatgcgg tgtacagcca acgtaaatat
     4621 tcacggtcta tcatctcttc cgttaaatag ccctgatcca caaaaaccac cggccattgt
     4681 ccgccttggg ctttatggca tgttacagac cacgcaaatt ttacctgcag cgcgttgtac
     4741 cagggatcgt tcatgatctt ttccttcctt aatttctggt cgggctcatc agaataatct
     4801 tccgagatcg cctcaaataa tgcctttagt ttttcggccg gtaaatgagg tccttctgtt
     4861 tgcaacgtat caataagaat ccgcatttcc acttcgggta aatcgggata atcgctcagc
     4921 tttacttcta catctgcaaa acgaagtcca tggtactctc gtatcgcctt cactttttga
     4981 actgtaagcg aatctccatt ggcaataaac cccgcaggtg aatcggcagg taaccagaaa
     5041 taattgtttt tcacagccat cagctcatct cctccgttca attcattttc tatccagaat
     5101 acacggttgc gcacctgcat attgaacagg tttgcccgct tgttggagcg tgtaataatg
     5161 cgaacgcctt ctgcaccata ctgaccaaaa gcccattcca gctcatcctg taattcggtc
     5221 ccggtaattt gtctgacatc aggtcttcct ttcgtttcta ttttgggaaa gccatttgca
     5281 ttggcagaaa gctgctcccg cagacgggta gcattgtaca agattcctga atcggaggct
     5341 tggcggacga cttgttttaa ctcaatggaa tgaacatcca agcggaaatt atcgcgtatg
     5401 aattcctcac tcaatgcagg actgtctgcc attccaaccg gtggtaactg tgcgggatcc
     5461 ccaactatca aggcaaaaca gttctttccc ctatagatgt accgtaacac atcttccagt
     5521 aaatccctgg atctgaaacc atcctgattt gaactacttc cggaaccaat catggaagcc
     5581 tcatcaatga tgaacagggt gttggaatgg ggattatgtg ccagttcaaa agaggaatat
     5641 cctgccttgt tcctcaacat gtaaatcttc ttgtgaatgg tataagccct ttgtccggta
     5701 tggcctgaca aaactttggc tgctcttcct gtgggtgcca atagttcggt attcattctt
     5761 aacattggca gaaagcgcac caactccccc atcatggttg tttttccggt tccggcatat
     5821 cccttcaaaa taaagattct atagcgctca gagccacaca ggaaatgaga aaaacggtcc
     5881 ataaacaact gctgatcgac cgttggctca atcctgagac tgcttcgaaa taattgggcg
     5941 atatgttccg ttctggagct gttcaaacgc accaatatta agcaacatcg gcttaataaa
     6001 ggtttaaaaa ggcgattgaa aaaaaattgt agatttgtcg gcactttgca taacagaaat
     6061 caactggcat gaacgcactt aaaaacattg gttacctagc tggcaagtat tttgcccaga
     6121 ttcttgtaat tacacttgga ttggttgtcc tgatgattgg catgaaaacc gatcccgaca
     6181 ccggattcaa acagaacagc ctcttcgtgc ttggcggtgt tgccttattg ctggcaggaa
     6241 caatttccac gctttatgtt gccgaagtga tcaaccgtat gcttcacacc atttttctgt
     6301 atgttttgct gcccattggt gttatcactt acggttacct cgattacaga tccattatgg
     6361 acgaagtgga ataccgcgaa agagttgcca tggtaaaccg tgaaacacaa caacgtctta
     6421 aggatatccg tgatgctcag gtggagttcc gtgcgatgta caaccgttat gcacctagtt
     6481 ttgattcact gcgctggttc attcaggaag gtcgtgcaat ggtaattaac cgtcttggcg
     6541 atg
//
LOCUS       DAOMYG010000145         6498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4061_length_6498_cov_8.922862, whole genome shotgun sequence.
ACCESSION   DAOMYG010000145 DAOMYG010000000
VERSION     DAOMYG010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4061_length_6498_cov_8.922862"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..472
                     /locus_tag="PK610_06050"
     CDS             161..472
                     /locus_tag="PK610_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HRJ35528.1"
                     /translation="MILYNVTVNIDEQVHEEWLQWMKDVHIPDVMRTGMFIENKICKI
                     LAESEGGVSYSVQYLCKDMPTFKKYESDFAPALRKDHSDKYGGRFAAFRTLLEVVHKV
                     N"
     gene            complement(487..3288)
                     /locus_tag="PK610_06055"
     CDS             complement(487..3288)
                     /locus_tag="PK610_06055"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ35529.1"
                     /translation="MVPEIILAQDFSVKGTLKDKSNGSPLPGANVVLIHRRDSSLIKG
                     STTDANGRFVITGLRRGGYRFQASFIGYSTLRKDTVMVFDDVNLGDLYLEPSSTELDD
                     ITVEERMVRVEIKGDTAEYNAKAFKVNPDATAEDLVKKMPGITSENGQMKVNGENLGR
                     VLVNGRPFFGEDPNAALRNLPANMVDKIQVFDQQSDQAAFTGFNDGTGTRTINILTNQ
                     KIGDGNFGKWYAAYGTDDYYNAGFSLNYFKGARKLSFVGMSNNINQQNFSQEDLLGVN
                     AANSSQGGGGSGGRGGPPQMAGMNWRGNQSGDFTNAAQSGIATTHSLGVNYSDRLGKK
                     AFLSGSYFGNYSDRFQEKWTNRNFIQSSDSSNNYTETSQSDNLTWNHRVNLRLDVNID
                     STSVLTWQPKGSFQSSVSDAVLNGSNTGAENLITTLMSNLNGSDMTAYVLGQTMTYRK
                     RLKKKGRTISAEWTSNYNSRYGDGYLNSRNVYFYSTDSLEILDQINDQNSLNHTQSVN
                     ITYTEPIKEKGQLQITYNPSFNWNTSNKTTLNYDSLASDHIVMDTSLTSKFITNYFVQ
                     KGGLLYRFTKDNNFNFQVGANAQYATLTSDQSFPMAFAIDKAFFNILPTASAYYAFNK
                     ESNIRLNYRTSTNQPNSNQLQDVIDNTNPILLRGGNPDLAQSFTHSLFGRWMKSKIIE
                     GTSLFWMMHASFTENYIGNSTFIATRDTVLRDNILLQRGSQYTSYANMSGNKSMRTYA
                     NYGFPMAPLKSNANVYASLGLNQTPGLVNGLKNISNNYSITGGFTLASNFSDKIDFTV
                     TTNSTYSIVRNSLQTNSDNNYLIQNSTGKLNWIFGKGFVFATDVTHSMYRGLQQEFNR
                     DFILWNAGLGYKFLKDRKLDVRLIAYDLLNQNNSINRQVTETYIEDTRSNVMNRYFQL
                     QLTYTFRNFNQQKPEEKKP"
     gene            3501..4289
                     /gene="rsmA"
                     /locus_tag="PK610_06060"
     CDS             3501..4289
                     /gene="rsmA"
                     /locus_tag="PK610_06060"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009746672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRJ35530.1"
                     /translation="MLFSRMVRPKKHLGQHFLIDLSIAHRVSDSLTGFGNYRQVIEVG
                     PGTGALTRFLLERKEFLTSVVEVDSESVEYLKEYFPQLNGRIYSEDFLRMKIGQLFDG
                     PLAVVGNFPYNISSQIVFKVIENRDQVPEMVGMFQKEMAERIAEPPGSKAYGIISVLT
                     QAFYDVEYLFTVHENVFNPPPKVKSAVIRLKRNNRNEMGCDPKFFIQVVKTAFNQRRK
                     TIRNSIKSLNPERSEHPYFDLRPERLSVEQFVELTNFLMPLPIS"
     gene            4494..5846
                     /gene="mgtE"
                     /locus_tag="PK610_06065"
     CDS             4494..5846
                     /gene="mgtE"
                     /locus_tag="PK610_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRJ35531.1"
                     /translation="MQFPITREIIDQLSEAIANGNDGFLMDFLKDLHGADIAEIINEL
                     DGDDGKYLFRLLENEQAADTLTELDEDVREELLESLTTREIAAHIEEMDSDDAADVVG
                     ELPEEKQREVISQIEDKAQVSDIEALLNYEEGTAGAIMAREFVKANLNWPVDRCIVQL
                     RKQAQEVEHVYTVYVVNDEDQLEGLLSLKSLLVANPKQTIGEVYQSKNIKFIQANQSI
                     EEAARLMERYDLVVLPVVDDQMRLIGRITIDDVVDVIREEAEKDYQMASGLSEKVESS
                     DTVWVISRARLPWLLIAMIGGIFGSMVIGQYEDVIGIHPEMAMFIPLIAAMGGNVGIQ
                     SSAIVVQGLAADEIQMQGMAARLFKELIIGMVNGLVCASVILGYSMLTADSLNLGFTV
                     SIALLTVICFAAVFGTFVPLLLNKYKVDPALATGPFITTTNDILGVTIYFLVGHFLYF
                     "
     gene            5945..>6498
                     /locus_tag="PK610_06070"
     CDS             5945..>6498
                     /locus_tag="PK610_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ35532.1"
                     /translation="MKVCFIDTVHPILKERLESRGVRCVMHTSTPQIELEQLIGSYDG
                     IVIRSRIKMDKRFLSKAANLKFIARSGAGMENIDLEFCEQKGIICFNSPEGNRDAVGE
                     QAIGMLLLLFRNILRADAQVRSGIWDREGNRGHELSGKTVGIVGFGNMGSALASKLTG
                     FGCRIVAYDKYKSGFATSTIEEVD"
BASE COUNT         1814 a         1190 c         1442 g         2052 t
ORIGIN      
        1 ggtttttaaa accccggagg tcttatggat ttgagacaaa gcaaagttcg gaggtgttgg
       61 ggataaaatc aatggaaaaa agaccaaaaa tgcagttaag tgaacggttc ggcagcggat
      121 tcgggtgttt tcttgttatt attgctgaaa atcgagagtg atgattcttt acaacgttac
      181 agtaaacatt gatgagcaag tccatgaaga atggcttcaa tggatgaaag atgttcatat
      241 tcctgatgta atgcgcacag ggatgtttat agagaataaa atctgtaaga ttctggctga
      301 gtcggagggg ggcgtatcgt attctgttca gtatttgtgc aaggatatgc ctacatttaa
      361 aaaatatgaa tcagattttg cacctgcatt gcggaaagac cattcagaca agtacggcgg
      421 tagattcgct gctttcagga ccttactcga agttgttcac aaggtgaatt aattactgct
      481 tgccaattat ggttttttct cttccggttt ttgctgattg aaatttcgga atgtgtaggt
      541 cagttgcaat tgaaaatagc gattcataac gttcgaacgg gtgtcctcaa tgtaagtttc
      601 agtcacctgg cgattaatgc tgttgttctg gttgagtaaa tcgtatgcaa ttagtcgtac
      661 atcaagtttg cgatctttca gaaatttgta tcccaatcct gcattccaaa ggataaaatc
      721 gcggttgaat tcctgctgga gtcctctgta catgctatgt gtaacatcgg ttgcaaaaac
      781 aaatcctttt ccaaaaatcc aattgagttt tccagtgctg ttttgaatca gataattatt
      841 atcggaattg gtttgtaaag aattccgcac aattgaatag gtggagtttg ttgtaactgt
      901 aaaatcaatt ttatcactga agtttgatgc aagtgtaaat cctccggtta tgctataatt
      961 gttggagatg ttctttaatc cgtttaccaa tccaggtgtt tgattcaatc caagacttgc
     1021 gtacacattt gcattgcttt ttaacggtgc catagggaaa ccatagtttg cataggttcg
     1081 catagatttg tttccgctca tgttggcata ggaagtatac tgtgatccac gctgcagtaa
     1141 aatattatcg cgcaacacag tgtctcttgt tgcaataaaa gtggaatttc caatgtagtt
     1201 ttcagtaaaa cttgcatgca tcatccaaaa cagggatgtg ccttcaatga tttttgattt
     1261 catccaacgg ccaaacaaac tgtgggtaaa cgattgcgca agatcgggat ttccgccacg
     1321 cagaagaatg ggattggtat tgtcaatcac atcctgcaac tgatttgaat tgggttgatt
     1381 cgtcgacgta cggtaattca aacggatatt actctcttta ttgaatgcat aatatgcaga
     1441 ggcagttggg agaatattga aaaaagcttt gtcgatagca aatgccatcg gaaaactctg
     1501 atctgatgtt aatgtagcat actgtgcatt ggctccaacc tgaaagttga aattattatc
     1561 cttggtgaat cgataaagca atccgccttt ctgaacaaaa taattggtta tgaatttact
     1621 ggttaacgaa gtatccatta caatgtggtc gcttgcaagg gaatcatagt tcagtgttgt
     1681 tttgtttgat gtgttccagt taaaactggg gttataagta atctgaagct gacctttttc
     1741 ttttatcggt tctgtatatg ttatgttaac actttgggtg tgattcaaac tgttctgatc
     1801 attgatttga tctaaaatct ctaatgagtc ggtggagtag aaatatacat tacgagaatt
     1861 caaatacccg tcgccatatc tcgaattgta attacttgtc cattccgccg aaatcgtccg
     1921 gccttttttc tttaatcgtt tgcgataggt catggtttgc ccaagcacat atgcagtcat
     1981 gtccgaacca tttagattac tcattaaggt agtaatcaga ttctctgctc cggtattact
     2041 tccattaaga acggcgtctg atacactaga ttgaaaactg ccttttggct gccatgtaag
     2101 cactgatgtg gaatcaatgt ttacatcaag acgcaaattc acccgatgat tccaggtgag
     2161 gttatcgctc tgactggtct ctgtgtaatt gttggaagaa tcactcgact gaataaaatt
     2221 ccggtttgtc catttttcct gaaaacgatc ggagtagttt ccaaaataac ttcctgataa
     2281 aaatgctttt tttccaagac gatcactgta atttacaccc agggaatgtg ttgttgcaat
     2341 accggattgc gctgcattgg tgaaatcacc ggattggttt cctctccagt tcatcccagc
     2401 catttgtggc ggaccacctc tgcctccgct tcctcctcca ccttgtgatg agttagccgc
     2461 atttaccccc aacagatctt cctgtgaaaa attctgctgg ttgatattgt tactcatacc
     2521 aacaaaagaa agtttccggg cgcctttgaa ataatttaaa ctgaatcctg cattgtaata
     2581 gtcgtcggta ccgtaagccg cataccattt accgaaattt ccgtctccga ttttctggtt
     2641 ggtaagaatg tttattgtgc gtgtacctgt tccatcgttg aaacccgtaa atgcagcctg
     2701 atcactttgc tgatcaaaaa cctggatttt atcgaccata ttcgcaggta aattacgaag
     2761 agcagcattt ggatcttctc caaaaaacgg acgcccgtta accaaaactc ttcctaggtt
     2821 ttcgccattc actttcatct ggccattttc agaagtgatt ccgggcatct tttttaccag
     2881 atcttcagca gtagcatcag gattgacttt gaacgccttt gcattgtatt ctgcggtatc
     2941 ccccttgatt tcgacacgta ccatgcgttc ttcgacggtg atatcatcaa gctccgtgga
     3001 gctgggttcc aaatacagat ctcccaggtt aacatcatca aataccatta cagtatcttt
     3061 tctcaacgtt gaataaccaa taaaactggc ctgaaaccga tagcctcctc tgcgcaatcc
     3121 tgtgataacg aatcgaccat tggcatccgt tgtactccct ttgatcagtg aagaatctct
     3181 acggtgaatc agtacaacat ttgctcccgg aaggggactt ccattggatt tgtccttaag
     3241 cgttcctttt actgagaagt cctgagcaag aatgatttca ggcaccatta aaagggtgaa
     3301 tagcaatagg tttcgaaaat accggagggt gatgtgatgt gtcatggcaa gtatagacgc
     3361 ttggttttgg aaatggttta atccgcgcct ggaattttta caaatctact cttaattgat
     3421 ctaatcgtgt gcgttttggg atgagcggta aggaattgca attgccaaca atttccattt
     3481 gaggatgaaa tgaatgtttt atgctatttt cacgcatggt tcgacctaaa aaacatctag
     3541 gacagcattt ccttatcgat ttatccattg ctcaccgtgt ttctgattca ctaacaggat
     3601 ttggcaatta ccggcaagtg attgaagtgg gtccagggac aggggctctt acccggtttt
     3661 tgttggagcg taaggagttt ctgacatcag tggttgaggt tgattcggag tctgttgagt
     3721 atttgaaaga atattttcca cagctaaatg gaagaattta ttctgaggat tttttgcgaa
     3781 tgaaaatagg acaactattt gatggtcctt tagctgttgt tggaaatttt ccttataata
     3841 tttcatcgca aattgttttc aaggtgattg aaaatcgtga tcaggtaccg gaaatggttg
     3901 gtatgtttca gaaggaaatg gcagagcgaa tagcagaacc tccgggaagt aaggcttatg
     3961 gaattatcag tgtactcaca caagcttttt atgatgttga atacttgttt acagtacatg
     4021 aaaatgtatt caatcctccc ccaaaagtta aaagtgctgt tattcgtctg aagcgaaata
     4081 atcgcaatga aatgggatgt gatccaaagt tttttatcca ggtggttaaa accgctttta
     4141 atcagcgcag aaaaaccatt cgcaattcca taaaatcctt aaaccctgaa cgaagtgagc
     4201 atccctattt tgatcttcgc ccggagcgat tgagtgttga acagtttgtt gagctgacaa
     4261 attttctgat gccattaccc atttcataat caatttcgga gtgaaattta attcgatttc
     4321 tgatatttta agtctcgaca aaggaattgg atcccttccc atttttgctt gcgactgtga
     4381 tctttaagtc acttattttg ttcagaaggt cgtcccatgc ggcgaccttt ttgtttgcag
     4441 ttgcctgact tttagtgggt tggttttact gattcaaaac ctatctttgc accatgcaat
     4501 ttccgattac aagagagatc attgaccagc taagcgaggc tatcgctaac ggtaacgatg
     4561 gttttctgat ggatttcctg aaggatctcc acggtgcgga tattgctgaa atcatcaacg
     4621 agcttgatgg agatgacgga aagtatttat tccgtttatt ggaaaacgag caagctgcag
     4681 ataccttaac agagcttgat gaagatgttc gcgaagaact tctggaatcc ttaactacac
     4741 gtgagattgc agcacacatc gaggagatgg attcggatga tgctgccgat gttgtaggcg
     4801 aattaccgga agaaaaacag cgcgaagtaa tctctcagat cgaagataaa gctcaggtaa
     4861 gtgatattga ggccttattg aattatgaag aagggactgc aggcgcaata atggcgaggg
     4921 aatttgtaaa agccaatctg aactggcctg tagatcgttg tattgttcaa ttgcgcaaac
     4981 aagctcagga agtagaacat gtttataccg tttatgttgt aaacgacgaa gatcaattgg
     5041 agggtttact ttcattgaaa tctctcctgg ttgccaatcc caagcaaaca attggtgaag
     5101 tctatcagag taaaaacata aaatttattc aggctaatca gtccatcgaa gaagctgcac
     5161 gcttaatgga acgttacgat ctggttgtat taccggtggt ggatgatcaa atgcgattga
     5221 ttggaagaat taccattgat gatgtggttg atgtaatccg cgaagaagca gagaaggatt
     5281 accaaatggc atcaggtctt tctgaaaaag ttgaatcttc agataccgtt tgggtgattt
     5341 cacgtgcgcg tttgccctgg ctgctgattg ccatgatcgg aggaattttc ggttcaatgg
     5401 ttattggtca atacgaagat gtgattggca ttcatcccga aatggccatg ttcattccac
     5461 tgatcgctgc aatgggaggg aatgtcggaa tacaatcttc cgccattgtt gtgcagggac
     5521 tggctgctga tgaaattcaa atgcagggaa tggccgctcg tttattcaaa gaacttatca
     5581 ttggtatggt aaatggattg gtttgtgctt ctgtgatttt aggttacagt atgcttactg
     5641 cagattcact caatcttggt tttactgtaa gcattgctct gttaacagtg atttgttttg
     5701 cagcggtatt cgggacattt gttccgcttt tgcttaataa atacaaagtt gatcctgctt
     5761 tggcaacagg tccgtttatt accacaacca atgatatcct gggtgtaacg atctattttt
     5821 tggttggaca ttttctttat ttctgatcag aataaagcgt atctaaattt ttcttacctt
     5881 tttaaacgat ctgttatgaa tttaattaat tggaaattgg taattagata attggtattc
     5941 gaaaatgaaa gtctgtttta ttgatacagt tcatccgatc ctcaaagagc gattggaatc
     6001 gcggggagta cgttgcgtta tgcatacttc aactccgcag atagaattgg aacagctaat
     6061 cggtagctac gatggaatag tgatccgcag ccggattaaa atggataagc gttttttgag
     6121 taaagctgcc aatctcaaat tcattgcacg tagtggagca ggaatggaaa acatagatct
     6181 cgaattttgt gagcaaaaag gaatcatttg ttttaattct ccggaaggca atcgtgatgc
     6241 agtaggtgaa caggctatcg gaatgttgtt gttattgttc agaaatattt tgcgcgcaga
     6301 tgcacaggtt cggtcgggta tttgggaccg tgaagggaat cgtggtcacg aactgtcagg
     6361 aaaaacggtt ggaatagtag gttttggaaa tatgggaagt gccctcgcat ccaagcttac
     6421 cggatttggt tgcaggatag ttgcatatga taaatacaaa agcggatttg cgacatccac
     6481 aattgaagag gttgattt
//
LOCUS       DAOMYG010000146         6458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4088_length_6458_cov_9.305950, whole genome shotgun sequence.
ACCESSION   DAOMYG010000146 DAOMYG010000000
VERSION     DAOMYG010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4088_length_6458_cov_9.305950"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PK610_06075"
     CDS             <1..270
                     /locus_tag="PK610_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35533.1"
                     /translation="GKWHLSYVDDSSVIDNPHIIGMEFREDGTIRQFDGGVSYEAKYI
                     ISDDTTSISVIEGGKPVTEFKLRLLTRERMEMEEGFHVVTFLKVK"
     gene            complement(347..1519)
                     /locus_tag="PK610_06080"
     CDS             complement(347..1519)
                     /locus_tag="PK610_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRJ35534.1"
                     /translation="MSRILIIDDEKPIRRALREILEYEKFEVDEAEDGPSGLEKAREG
                     NFDVVLCDIKMPKMDGLEVLETLQKESVDSPVVMISGHGNIETAVEAIKKGAFDFIQK
                     PLDLNRILVTIRNAMDRVELVQETKSLKQKISKGKSSEIIGESKAIAEIKDMIDRVAP
                     TDARVLITGPNGSGKELVARQLHEKSARNKGPFVEVNCAAIPGELIESELFGHEKGAF
                     TSAIKQRLGKFESAEGGTLFLDEIGDMSLSAQAKVLRALQENRITRVGGEKDIKVNVR
                     ILAATNKDLRKEIENNNFREDLYHRLSVILIHVPALNDRKDDIPLLVEHFSKLICEEQ
                     GMPVRSFDKGALKALQELNWTGNIRELRNVVERLLILGGKSISEADVKAYAVPRHG"
     gene            complement(1626..2444)
                     /locus_tag="PK610_06085"
     CDS             complement(1626..2444)
                     /locus_tag="PK610_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35535.1"
                     /translation="MRFLFSLLFVFQSCLLLAGNDEPFTQKGYINVGFRWNKYQTERS
                     IYYPAFNNSLSRQDTIGADFSGKNTCFYFNMERMERFLYWEMDMAVSGKGEGELENYN
                     YFDAQSSHFSEQNHQLNFRSARFDVGLYLGGLIKGKFGVFLGGHYGYNNTFVSGYPTS
                     DYKISQYGGVNRGLGGMLIFPFIKGQFRFTLLFNKIDNNELKLNATGNTAGFVWNIHF
                     DKRRSGGLTIGYYTNNLRFKDYPFKAYDGSDSHEFKHGYFQLGILFPFFHWLNE"
     gene            complement(2512..3435)
                     /locus_tag="PK610_06090"
     CDS             complement(2512..3435)
                     /locus_tag="PK610_06090"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35536.1"
                     /translation="MKYAFLDLGTNTFNLLVVELTEKGIRKLHVGRIPSRLGKGGMKE
                     KMITPEAFDRGIQAIKEHQSTALQYGEVKFHAFATSMIRDAGNGPEFVKQVLLETGIT
                     INVVNGEREAELIFKGVCQAFRSEQPFLIMDIGGGSTEFIIAQNKELLWKKSYQLGVT
                     RLYEQFPHSDPMNSAEKETTEKHLFEVMTELFEQMKKYNVQTLVGSAGSFESYAEIIH
                     RRSFGKGIDLSIPAHHIPGEEFKSLLQEVLKSDHAQRTRLEGLIEFRRDLIVMASLLI
                     DTIFRSHPFNEVYFSVYSLKEGIMAELIGTE"
     gene            complement(3531..5135)
                     /locus_tag="PK610_06095"
     CDS             complement(3531..5135)
                     /locus_tag="PK610_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35537.1"
                     /translation="MKRFFKTIPFQSVFVLLLCTQTIFAATTIPTRGYTGKRFSMQGS
                     DASSQFIQHSNIGMPLKGYVVGNDGNVTEHVIAYQEPEKLLAHEFDLAICREANGKKV
                     DVLNPQSEPNFASWVKKDQLSAFYVGDQLFVKGSKNKWYILMSEGAIREIVSLTKVVN
                     NGVTGYAVTEMIQKLDQEPVGVTGLTLSFKNTMSSMTNEHTVLSEKIKKKESGYRLLN
                     LNTVLKEYNTWYNLNYPGQVNYVMPYSYSIPVSTTDELGELFFTSIKDKNYDIIVGLT
                     PSMNDFLATAKSKTTDPEQLKQAEKELKPSMNKIVMDKNKESFFALLNEFATADRSQM
                     QLEKFVFTKNESRSQSSNIDQGQGELHFTANNTPYRIAIKKMFRLNSGWKISEIVLHK
                     TDVISSVEEPAPEEPKEEIAEAPARYDLPEKFSGLLGEWTFVKAIMNGKDVSKEFRQF
                     YIHDQAITLKYLFGGKIIYPVQAETELKITHGYWTYNPNEERDLQIITYMTNGSATER
                     FKIISITDKEFIYMDPKMKTTFYFKR"
     gene            complement(5285..>6458)
                     /locus_tag="PK610_06100"
     CDS             complement(5285..>6458)
                     /locus_tag="PK610_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ35538.1"
                     /translation="ELGLTHQVFKDKPNVVEFDYRTTHLEAKEFLEGDQYDLALYINK
                     NTINGDKALLLYKKIPSLSIQSYINSELDAVMESYRLMKANTGPEVQKFINEEYPKIK
                     RHIELETKNAESGEKAKTKHYAAVVGYVFALLIYMFIFLYGVQVMRGVIEEKTNRIVE
                     IIISSVKPFELMLGKIIGIAMVGLTQFILWVLLTGLILILAQSLFFPDFYDAANQANF
                     TQVANGISPEQAAINFESNEIYDLIFIRINWALMLFMFLFYFIGGYLMYSALFAAIGA
                     AVDSESDTQQFMMPITIPLVFGFIVAQMAIENPESNAVFWSSMIPFTSPVVMMVRVAM
                     GFEPGTIWQLYLSMALLVAGFITCTWIAGKIYRTGILMYGKKVSYKELFKWMFYKG"
BASE COUNT         1656 a         1386 c         1419 g         1997 t
ORIGIN      
        1 ggcaaatggc atctgtcgta tgtcgatgat tcctcagtga tcgacaatcc gcacattatc
       61 ggcatggagt tccgtgagga tggaaccatt cgtcagttcg atggcggtgt gagctacgaa
      121 gcgaaataca tcatcagcga cgataccact tctatttctg taattgaggg aggcaaaccg
      181 gtaaccgaat tcaaattgcg tttactcacc agagagcgca tggaaatgga agaaggtttc
      241 catgtggtca cttttctcaa agtgaagtga gcgcattcgc tgcaagaaat ttttccagtt
      301 gttcaatgac ggtttccgga tgtaaacaca ttcttccgta ttcggattat ccatgccttg
      361 gtacggcgta ggcttttaca tcggcttcgc taatgctttt tcctccaaga ataagcaagc
      421 gctccaccac attgcgcaat tcgcggatgt tgcctgtcca gtttaattcc tgcagtgctt
      481 ttaatgcacc tttatcaaac gaacgcacgg gcatgccttg ttcttcgcag atcagtttgc
      541 tgaagtgttc tacgagcagc ggaatatcgt ccttgcggtc gttgagtgcg ggaacgtgga
      601 tgagaataac gctgagacgg tggtagaggt cttcgcggaa attattgttt tcgatttctt
      661 tgcgaagatc tttattggtg gccgcgagaa tacgcacgtt aaccttgatg tctttttctc
      721 cgcctacacg tgtgattctg ttttcctgta aggcacgcaa cactttggcc tgtgccgata
      781 aactcatatc gccgatctcg tcgagaaaca aggttccgcc ctctgcactt tcgaattttc
      841 cgagacgttg ttttatcgcc gatgtaaacg ctcctttttc gtgaccaaac aattcactct
      901 cgatcaattc gccgggaatg gctgcgcagt tgacttctac aaagggacct ttattgcgtg
      961 cagatttttc atgaagctga cgtgctacca attccttacc acttccgttc ggaccggtaa
     1021 tgagcacacg cgcatcggtg ggtgcaacgc gatcgatcat gtctttgatc tcggcaatag
     1081 cttttgattc tccaatgatc tcactcgatt ttcccttgct gattttttgt ttgagcgatt
     1141 tggtttcctg taccagctca acacgatcca tggcattgcg aatggttacc agaatacggt
     1201 taaggtcgag tggtttctgt ataaaatcga atgctccttt tttgatggct tcaacggcag
     1261 tttcaatatt gccatgaccc gagatcatca ccacgggaga atcaacactt tctttttgca
     1321 gggtttccaa aacttccagt ccgtccatct ttggcatttt gatatcgcac aataccacat
     1381 caaaatttcc ttcccttgct ttttccagtc cagatggacc atcttctgct tcgtccactt
     1441 caaacttctc gtattccagg atttcacgaa gtgctcttcg aatgggtttt tcatcgtcga
     1501 ttatgagaat tctggacata gtaacgggtt tgagttccct caaatatact acgagaatgg
     1561 caattgtgcg aaccgccctg aattttgccc gggcagcagc ttttttccct ttgtgaagca
     1621 ggaatttatt cgttcagcca gtgaaagaag gggaagagaa ttccaagctg gaaatagccg
     1681 tgcttaaact catgcgaatc gctgccatca taagctttga agggataatc cttaaagcga
     1741 aggttattgg tgtaataacc gatggtaagg cctcccgaac gtcttttatc gaaatggatg
     1801 ttccacacaa agcctgcagt attgccggtt gcgtttaatt ttagttcgtt gttgtcgatt
     1861 ttattaaaga gcagtgtaaa gcgaaattgc cctttgatga aaggaaaaat caacataccg
     1921 ccaagaccgc ggttcacgcc tccgtattgc gaaattttat aatcgcttgt gggataaccg
     1981 gagacaaagg tgttgttgta tccgtaatga ccgcccagaa atacgccaaa ttttcctttg
     2041 atcaatccac ccaaatacaa accgacatca aagcgtgcgg agcgaaaatt gagttggtga
     2101 ttttgttcgc tgaaatgact gctttgtgca tcgaaataat tgtaattctc caattctcct
     2161 tcaccttttc cgcttactgc catatccatt tcccaataga gaaaacgctc catacgctcc
     2221 atgttgaaat aaaagcaagt gttttttccg gaaaaatctg cgccaatggt gtcttgccgg
     2281 ctcagtgaat tattgaatgc aggataataa atggaccgct cggtttggta cttgttccag
     2341 cgaaaaccga cattgatgta tcctttttgg gtaaaaggct catcgttgcc ggccaatagc
     2401 agacaacttt ggaaaacaaa caagagagaa aataaaaagc gcataagatt ccgtttaata
     2461 cgacgaaact agcttggaaa tctgagaaag gaactaaggg aattgtcgta tttattcggt
     2521 cccgatcagt tcggccatga tgccttcttt cagtgaatac accgagaaat atacctcatt
     2581 gaagggatgc gatcggaaaa tggtatcgat aagcaaactt gccattacaa tcaggtcgcg
     2641 acggaactcg atcaatccct cgagccgggt gcgttgggca tgatcgcttt tcagtacttc
     2701 ctgcagcagc gatttaaact cttcaccggg gatatgatgt gcgggtatgc ttaaatcgat
     2761 tcccttgcca aatgatcttc ggtgaatgat ctctgcataa ctttcaaaac ttcctgctga
     2821 accaaccagt gtttgtacat tgtacttttt catctgctca aataattctg tcattacttc
     2881 gaacaaatgt ttttcggttg tttctttttc tgcactgttc atcgggtccg aatgcgggaa
     2941 ttgctcatac aaacgtgtta caccgagctg atagcttttt ttccagagta attccttgtt
     3001 ttgcgcgatg ataaattcgg tgctgccacc gccaatgtcc atgatcagaa atggttgttc
     3061 agaacgaaat gcctgacaaa cgcctttaaa gatcaactcg gcttcccgct ctccgtttac
     3121 gacattaata gtaattccgg tttcaagtaa tacttgtttt acaaattcag gaccattacc
     3181 ggcatcacgg atcatggatg ttgcaaaggc atggaatttt acttctccgt attgcaatgc
     3241 agtcgactga tgttctttta tcgcttgtat tcccctgtcg aatgcttcgg gtgtgatcat
     3301 cttctctttc attcctcctt ttccaagtcg tgagggaatg cgtccaacat gcagtttgcg
     3361 gattcctttt tcggttaatt caactaccag gagattgaac gtattggtgc cgagatcgag
     3421 aaatgcgtat ttcatcgtca aaagataata aagcttttga tacacggcaa aacagcacat
     3481 aaaaaaaggc gatccctttc gggaccacct tttttttcag ggtataggac ttatcgttta
     3541 aagtagaaag tggttttcat ttttggatcc atgtaaatga attccttatc ggtaatactg
     3601 atgatcttaa aacgctctgt agctgatccg ttggtcatgt aggtgatgat ctgcagatcg
     3661 cgttcttcgt tggggttgta tgtccaatac ccgtgtgtaa tctttaattc cgtttcggct
     3721 tgtacgggat agatgatctt tccaccgaaa aggtatttta aggtgatggc ctggtcgtga
     3781 atgtagaact gacgaaattc tttggaaaca tcctttccgt tcatgatggc ttttacgaat
     3841 gtccattccc ctaatagccc gctgaatttt tcgggtaaat cgtaacgggc gggtgcttct
     3901 gcaatttctt ctttgggttc ttccggagca ggttcttcaa ccgaggaaat tacatccgtt
     3961 ttgtggagta cgatctcaga aattttccat ccgctgttca gacgaaacat ttttttgata
     4021 gcgatccggt aaggcgtgtt gttagctgta aaatgcaatt cgccctggcc ttgatctatg
     4081 tttgagctct gactgcggga ttcgttttta gtaaagacaa acttttctaa ttgcatttgc
     4141 gaacgatctg ctgtggcaaa ttcattgagc agtgcaaaaa aggattcctt gtttttatcc
     4201 attacaattt tgttcatgga aggctttagt tctttttctg cctgtttcaa ttgttcggga
     4261 tctgttgttt tgcttttcgc tgttgctaaa aaatcattca tgctgggagt aagtccaacg
     4321 atgatatcat agttcttatc cttaatggaa gtgaaaaaca actcgcccaa ttcatcagtt
     4381 gtggaaacag gaatgctgta ggaatagggc atcacataat ttacctgtcc gggataattg
     4441 aggttgtacc aggtattgta ttctttaagt accgtattca gattcagcaa tcggtaaccg
     4501 gattcttttt tcttgatctt ttcggaaagc actgtgtgtt cgttggtcat tgaactcatg
     4561 gtgtttttaa aacttagtgt gagtccggta acgcccaccg gttcctgatc gagtttctgg
     4621 atcatttcgg ttacagcata tcctgttaca ccgttgttga ccaccttggt gagggaaacg
     4681 atttcacgaa tcgcaccttc cgacatcagg atgtaccatt tgtttttgct tccttttacg
     4741 aagagctgat cgcccacata aaatgcactg agctgatctt tttttaccca gcttgcaaaa
     4801 ttgggttcgc tttgcggatt gagcacatcc actttttttc cgttggcttc acgacagatg
     4861 gccagatcga attcgtgcgc cagtaatttt tcgggttcct gataggcaat tacatgttct
     4921 gttacattgc catcgttacc tacaacatat ccttttaacg gcataccaat gttgctgtgc
     4981 tggataaatt gagaagaagc atccgatccc tgcatcgaaa aacgtttgcc ggtgtagccg
     5041 cgtgttggaa ttgttgttgc tgcaaaaatt gtttgggtgc aaagcagcag cacaaaaacc
     5101 gattgaaagg gaatcgtctt aaaaaagcgt ttcatactaa tttgggttgg ttagtattac
     5161 caaattaggg tgaaaatccc gctaattgat cctgtttttg caggatgaca ggtatttttt
     5221 acgaatggaa aagctaaaac ggttcaaata ggaaaaaact gatacttatc agttcaaggg
     5281 cgactcatcc tttgtagaac atccacttaa agagttcttt gtagctcact tttttgccgt
     5341 acatcagaat tccggtgcgg tagatttttc cagcgatcca ggtgcaggta atgaatcctg
     5401 ctaccaatag cgccatactc aggtacaatt gccaaatcgt tccgggttca aagcccatgg
     5461 caacacgaac catcatcact accggagaag taaagggaat catgctcgac cagaatactg
     5521 cattgctttc gggattttcg atggccattt gcgcaacgat aaatccgaat acaagcggta
     5581 tggtaatcgg catcataaat tgctgcgtat ccgattcact atccactgct gctccgatag
     5641 ctgcgaagag tgcgctgtac atgaggtatc cgccaatgaa gtagaaaagg aacatgaaca
     5701 acatcagcgc ccagttgatg cggatgaaaa taagatcata gatctcgttg ctttcaaaat
     5761 tgatggccgc ttgttccgga ctgatgccat tggcaacctg cgtaaagttg gcttgattgg
     5821 ctgcatcgta aaaatcgggg aagaagagcg attgcgcgag aatgagaatg agtccggtta
     5881 acaataccca caaaataaac tgggtgagtc caaccatcgc aataccgata attttaccca
     5941 acatcagttc aaacggtttt accgatgaaa tgatgatctc cacaatacgg tttgtttttt
     6001 cttcgatcac accgcgcatg acctgcacac cgtacaggaa gatgaacatg taaatgagca
     6061 gagcaaaaac atatccaact actgcggcat aatgtttggt ttttgctttt tctccgctct
     6121 ctgcattttt tgtttcgagc tcgatgtgtc gtttaatttt cgggtattct tcgttaatga
     6181 atttttgaac ttccggtccg gtgttcgctt tcattaagcg gtaactttcc attaccgcat
     6241 ccaattctga attgatgtag gattgaatgg agagtgaagg aatctttttg taaagcaaca
     6301 atgctttatc gccgttgatg gtgtttttgt tgatgtataa ggcgaggtcg tactgatcgc
     6361 cttccagaaa ttcttttgct tcgaggtgag tggtgcggta gtcgaattct actacgttgg
     6421 gtttgtcttt gaacacctga tgcgttaatc cgagttca
//
LOCUS       DAOMYG010000147         6432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4113_length_6432_cov_7.386075, whole genome shotgun sequence.
ACCESSION   DAOMYG010000147 DAOMYG010000000
VERSION     DAOMYG010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4113_length_6432_cov_7.386075"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PK610_06105"
     CDS             <1..307
                     /locus_tag="PK610_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35539.1"
                     /translation="SASYLLHYSTFSMMREQIMASSSTIFQDDTGIPYKHFKSASSWR
                     TVLYGNYTKPVKDFGSFTFQDDLDSVYSASKGLPSLPFHLGYHWGDRKQSQQLFIRK"
     gene            304..621
                     /locus_tag="PK610_06110"
     CDS             304..621
                     /locus_tag="PK610_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35540.1"
                     /translation="MKTRFFLLLLFFVTVSVGNANAIGEVSNEQYWKDVRKRMPEFSK
                     IFKKHKDFRLEILVTQIDRNAANGIERLKRYHFGDSLSYFYPASIVKLPASIFCWRKF
                     VNS"
     gene            594..1703
                     /locus_tag="PK610_06115"
     CDS             594..1703
                     /locus_tag="PK610_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRJ35541.1"
                     /translation="MLEKIREQLKNGSVESFVYFDSAFFCGNNRYENYYPLYHRVTQK
                     ESFRDFAKRMHLPADYVFKLNAHLHPDSLLPLNTSIRLSEWKYQLTLRDLLIAMLVYS
                     DNDAFNKVYEIISQDFFLMRFREMNYKSRVVRRLMYCPPGGDTLAPEYTLTDRDSVTV
                     LVQTRSKSLSSRMRFESEPVLVGKRHMVDDRIVKGGKDFANHNWVRLNEMHYLLEKLV
                     LHEHLDQDKRLRIGPFEQRTLIRLLGNHPREITRMRTKSFDGLPDAYLNFLLFGGTEE
                     TIPDNLRSINVAGWAFGFTTDCAYIIDTKTKSEIFISARIYTNKDKVIGNDKYEYDKI
                     ARPFLKRLGQVVLEKEQKREKETLPHLMSVAGLFK"
     gene            complement(1696..2139)
                     /locus_tag="PK610_06120"
     CDS             complement(1696..2139)
                     /locus_tag="PK610_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HRJ35542.1"
                     /translation="MMKQTLVKLILAGVFSFSPLYISVHYIFLSDETTATFIPELTLE
                     NVYKELIRKEIQHPEIVIRQVIAETQWLKCRNCSKQFGNLFGFTTKQGYLKFDNWVAS
                     IAYYKQWQDAYYKGGDYYQFLHRIGYATAPNYIRLLKQIRLPQLT"
     gene            2269..2721
                     /locus_tag="PK610_06125"
     CDS             2269..2721
                     /locus_tag="PK610_06125"
                     /inference="COORDINATES: protein motif:HMM:NF017434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HRJ35543.1"
                     /translation="MNQSLTATKKILLGGKEKVHFEEINKHNVLARIDTGAKTSSIHC
                     EKVWVEREGNKKVLCAFLLRKTTQVTRFKKFTMKEVKSSNGISQKRYAIKLRVRIGDF
                     ETLTEFTLTNRDKMSYPVLLGRKVLRKAFIVDVSRNFIQSGDGTPAEN"
     gene            2805..3710
                     /gene="rimK"
                     /locus_tag="PK610_06130"
     CDS             2805..3710
                     /gene="rimK"
                     /locus_tag="PK610_06130"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HRJ35544.1"
                     /translation="MKIAVLSRNSKLYSTRRLVEAAKMRGHDVVVLDHLSCTMLNEHG
                     KPRMFYRGQEILGIDAVIPRIGSSVTFYGTAVVRQFEMMKVFSVNSSVSITRSRDKLR
                     SLQIFSRHDIGIPKTVFANHPKDIDNLLRLVGGSPVVIKLLEGTQGIGVVLAETEKSA
                     KSIIEAFYGLDASFLVQEFIEEAKGADIRCFVVGGRVVGAMRRQGLEGEFRSNLHRGG
                     KAEVIKITRDERRAALLAAKAMGLNVAGVDMLQSKRGPLILEVNSSPGLEGIEGATGV
                     DIAGRIIEFIESKFHQGYKKDKIVG"
     gene            complement(3714..5801)
                     /gene="argS"
                     /locus_tag="PK610_06135"
     CDS             complement(3714..5801)
                     /gene="argS"
                     /locus_tag="PK610_06135"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRJ35545.1"
                     /translation="MTPEILLREKIIACIETIQPGIGMHDQVVLQKTKKEFTGDLTLV
                     TFPFAKALRKSPEETSELIGRHLKEYSGIVTEYQVVKGFLNISLADSLWIATLATALK
                     KDFGKAEQASGKTYLVEYSSPNTNKPLHLGHLRNNFLGYSVSEILKANGHNVVKTQVI
                     NDRGIHICKSMLAWKKSGKNETPQSSGLKGDKLVGKYYVEFDKQLREELKPILQSIYE
                     KGDLSLFNEKEKATITAHLLKHVQLNEVLSKEMSTVNATSFQVFGGEIQKMTEELIEK
                     KSGAKSLLKSFESFEKPDNQQKELIKQLRSIAGIESKISDIAGEIKELAQNKTALMQE
                     AQQMLLLWEKGDKETIALWEKMNAWVYEGFNATYKTIGVDFDKLYYESSTYLLGKEKV
                     LEGLSKGLFFKKEDGSVWCDLTADGLDEKLVLRGDGTSVYITQDIGTALLRYDDFPAM
                     NGMIYTVGNEQDYHFKVLFLILKKLGYSWANECFHLSYGMVDLPSGKMKSREGTVVDA
                     DDLAEEMVREAESKTRELGKYDEIPSEEREPLFHTLGLGALKYFILKVDPRKRMLFDP
                     SESIDFQGNTGPFLQYTHARIRSLLRKAENPAYAYDAVQHLLPEEREILKTLTEFPEV
                     IAEAGKNFSPALIANYIYELVKCYNHLYQNTVILKEEDESKRSLRLALSEATANAIRN
                     GMQLLGINVPEKM"
     gene            complement(5798..>6432)
                     /locus_tag="PK610_06140"
     CDS             complement(5798..>6432)
                     /locus_tag="PK610_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HRJ35546.1"
                     /translation="KAHPDKRLGVVWVDAHADLHSPYTTPSGNLHGMPLATALGNDNL
                     EDKINDIQEETRLGWEKLKNVGGISPKILPTDLVFIGVRDTEPAEDHVMVNNNIRNFK
                     VNEVRQKGTAKVVQETLEYLKACDLIYVSFDVDSMDPEVSRGTGTPVAGGFTAEEAHE
                     ITAGLVSSSKCSVFEIVEVNPCLDDKINKMAETAFSIVKNIVAVADKKSI"
BASE COUNT         1742 a         1301 c         1361 g         2028 t
ORIGIN      
        1 gtcggcatcc tatttattgc attattcaac gttttcaatg atgcgtgaac aaattatggc
       61 atccagctcc acgatatttc aggacgatac agggattccg tacaaacatt tcaaatcagc
      121 ttcgtcgtgg cgaacggtgc tatatggtaa ttacaccaaa ccggtaaagg atttcggttc
      181 gtttacattc caggatgatc tggattctgt ctattccgct tctaaaggat taccatcgct
      241 accctttcac cttgggtatc attggggcga cagaaaacag agtcaacaat tatttatcag
      301 aaaatgaaga cacgattttt tcttctgctt ttattttttg taacagttag tgttggaaat
      361 gcaaacgcca ttggagaggt ttccaacgag caatactgga aagatgtgcg aaagagaatg
      421 ccggagtttt cgaaaatatt taaaaagcat aaggatttcc gtttggagat tctggtaacc
      481 caaattgaca gaaatgcggc aaatgggatt gagcggttga agcgctacca ttttggtgat
      541 tcgctcagct atttttaccc tgccagtatt gttaaattgc cggcttccat tttttgctgg
      601 agaaaattcg tgaacagcta aaaaatggtt ctgtagaatc atttgtctat ttcgattctg
      661 cctttttctg cggaaataac cgctatgaaa attactaccc gctttatcac cgtgttaccc
      721 aaaaggagag ttttcgcgat tttgcaaagc gcatgcatct gccggcagat tatgttttta
      781 aattgaatgc gcatttacat cccgattctt tacttcctct gaatacttct atccgccttt
      841 cggaatggaa atatcaactg actttacggg atcttttgat tgccatgttg gtgtacagtg
      901 ataatgatgc tttcaataag gtttatgaaa taatcagtca ggatttcttt ttaatgcgat
      961 tccgggaaat gaattacaaa tcaagggtag ttcgccgttt gatgtattgt cctccgggtg
     1021 gcgatacgct tgctcccgaa tacacactta ctgatcgcga ctctgttact gttttggtac
     1081 aaacccgaag caaatccctt tcctcacgta tgcgatttga atcggagccg gtactggtgg
     1141 ggaaacgtca tatggtggac gacaggattg taaagggcgg aaaagatttt gcaaaccaca
     1201 attgggtgcg cttaaatgaa atgcattacc tcctggaaaa gcttgtgctt catgaacacc
     1261 tggatcagga caaacgatta agaattggtc ctttcgaaca acgcacgctt atacgtttgt
     1321 tgggcaatca tccgcgggag atcactcgta tgcgtaccaa atcatttgac ggattacccg
     1381 atgcttactt gaatttcctg ttgtttggcg gtaccgaaga aactattccc gataacctgc
     1441 gaagtatcaa tgttgcagga tgggcatttg gatttacaac agattgtgcc tacatcatcg
     1501 atacgaaaac aaaatccgaa atttttattt ccgcaaggat ctacaccaat aaagataaag
     1561 tgatcggtaa cgataaatac gaatacgata aaattgcgcg cccgttttta aaacgactgg
     1621 gacaagttgt tctggaaaag gagcagaaac gcgaaaaaga aacactccct catttgatga
     1681 gtgttgcggg tctctttaag taagctgtgg caaacggatc tgtttgagca gtcgaatata
     1741 gttcggtgca gtagcgtatc cgatcctgtg aaggaactga taataatctc cgcctttata
     1801 ataagcgtcc tgccactgct tataataagc gattgaggct acccaattgt caaatttcag
     1861 gtatccttgt tttgtggtga aaccaaacag gttaccgaat tgcttcgaac agttgcggca
     1921 tttcaaccat tgggtttcgg caattacctg gcgaatgaca atttcaggat gctgaatctc
     1981 tttacggatc aattccttgt acacgttttc cagcgtcagt tcgggaatga acgttgccgt
     2041 tgtttcgtcc gataaaaaga tgtagtggac agagatgtaa agcggggaaa acgaaaagac
     2101 cccggcaagg ataagtttaa ctaaggtttg cttcatcata gcttaatcat tgactagcgg
     2161 gtgcaaaggt cgaccattca gcttccttat caaaccttgt tttttgaaca gcgtcatgtt
     2221 ttctgtcatt ttgacgtata tttaacattc attatcaaaa aatacgctat gaatcaatcg
     2281 ttaacggcga caaaaaaaat cttgcttggt ggaaaagaga aggttcattt tgaagaaatt
     2341 aacaaacata atgtgcttgc gcgcatagat acgggtgcga aaacttcttc aattcactgc
     2401 gaaaaagtgt gggtggaacg tgaaggaaac aagaaagtgc tctgtgcatt tttattgcgc
     2461 aaaacaacac aggtaacgcg gttcaagaaa ttcacgatga aagaagtaaa aagctccaat
     2521 ggtatttcgc aaaaacgata tgctattaag cttcgcgtac gcattggtga ttttgaaaca
     2581 cttacagaat ttacacttac caatcgcgat aaaatgagtt atccggtttt gttgggacgc
     2641 aaggttttac gcaaggcgtt tatagtggat gtcagccgga actttattca gtcgggcgac
     2701 ggtactcctg ccgaaaatta atcgctaaag ttttttttga ggactttgtt ttttgcattc
     2761 atccttttct gattttagta atgccataag acaaggcaac tgttatgaag atcgctgtat
     2821 tatcccggaa ttcgaaattg tattcgacgc gacgtttggt tgaagccgca aaaatgcgtg
     2881 gtcatgatgt ggtggtgctc gatcaccttt cctgtacaat gctgaacgaa catggtaagc
     2941 cccgcatgtt ttatcgtggt caggaaattc tcggaattga tgctgttatt ccgagaattg
     3001 gttcatcggt taccttttac ggaacagctg ttgttcgtca gtttgaaatg atgaaagttt
     3061 tcagtgtgaa ttcatccgtt tcaattaccc gttcgcgcga taaacttcgt tcgctgcaaa
     3121 ttttttcccg tcacgatatc ggaattccga aaacagtttt tgccaatcac cctaaagaca
     3181 ttgataatct tcttcgtctg gttggtggat cacccgttgt aataaagttg ttggaaggaa
     3241 cacagggaat tggtgttgtt ctcgcagaaa cggaaaaatc agcaaagtcg attatcgaag
     3301 ccttttacgg tttggatgcg tcgtttcttg ttcaggaatt cattgaagaa gcaaaaggag
     3361 cagatatacg ctgttttgtt gttggcggaa gagtcgttgg cgcaatgcgt cgtcagggac
     3421 tcgaaggtga gtttcgctca aacttgcatc ggggtggtaa agcggaagtc attaaaataa
     3481 cccgggatga gcgtcgcgct gcattacttg ctgcaaaggc gatgggatta aatgttgccg
     3541 gagtagatat gttacaatcc aaacgcggac cattgatcct tgaagtgaat tcctcacccg
     3601 gactggaagg tattgaaggt gcaacgggag tggatattgc aggacgaatc attgaattca
     3661 tcgaatccaa gtttcatcaa gggtacaaaa aagataagat tgtaggataa tccttacatt
     3721 ttttcaggaa cgtttattcc caataattgc attccgttgc gaatggcatt tgccgttgct
     3781 tcggagagtg caagtcgtag tgatcgtttc gattcatctt cttccttcag aataacagta
     3841 ttctggtata aatggttgta gcactttacc agttcgtaga tataatttgc aatcagtgcc
     3901 ggcgagaaat tttttccggc ttcagcaatt acctcgggaa actcggtgag tgttttcaga
     3961 atttcacgtt cttccggaag cagatgttga acggcgtcat atgcataggc aggattttct
     4021 gctttacgca acagagaacg aatgcgtgcg tgggtatact gtaaaaacgg accggtattc
     4081 ccctggaaat cgatagactc tgaaggatcg aataacatgc gttttcgtgg atctactttg
     4141 agaataaaat attttaatgc tcccaatcct agtgtatgaa agagaggttc gcgttcttcg
     4201 gaaggaattt cgtcgtattt cccaagttct cgagtttttg attctgcttc acgcaccatt
     4261 tcttcggcaa gatcatcagc atccacaaca gttccttcac gcgatttcat ttttccggaa
     4321 ggaagatcga ccattccata ggataaatga aaacactcat ttgcccagga gtatcccagt
     4381 ttttttaaaa tgagaaacaa cactttgaaa tgataatcct gctcgttacc taccgtgtag
     4441 atcattccgt tcatagcagg gaaatcatca taacggagaa gtgcggtacc gatatcctga
     4501 gtgatataca cagacgtacc atcgcctcgc aaaactaatt tttcatcaag accatctgca
     4561 gtaaggtcac accaaactga tccatcttcc tttttgaaaa acaaaccttt gcttaatcct
     4621 tccaacactt tttctttacc aagaaggtag gtgctggatt cgtaatagag tttgtcaaaa
     4681 tcaacaccta tggttttata cgtcgcattg aatccttcat agacccatgc attcattttc
     4741 tcccaaagtg caatcgtttc tttatcgccc ttttcccaga gcaaaagcat ttgttgcgct
     4801 tcctgcatca gggcagtttt attttgtgcg agttccttta tctcacccgc aatatcactt
     4861 attttcgatt ctattcctgc aatggaacgt agttgtttga tcagttcctt ttgctgatta
     4921 tcgggttttt caaacgattc aaaactcttt aacaaggatt ttgctcctga ttttttttca
     4981 atcagttctt cggtcatttt ttgtatttca ccaccgaata cctgaaaaga tgtagcattt
     5041 accgtactca tctcttttga aagtacttca ttcagttgca catgttttaa aagatgcgcg
     5101 gtgatcgttg ctttttcttt ttcgttaaag agcgacaaat cacccttctc gtaaattgac
     5161 tgaagtatag gttttaattc ttcccgcagc tgtttgtcaa attccacata atatttccca
     5221 accagtttat ctccttttaa tccgctgctt tgtggtgttt catttttacc tgattttttc
     5281 caggcaagca tggatttaca aatgtgaatt ccacgatcat tgatgacttg tgtctttact
     5341 acattatgac catttgcttt cagaatctcg cttaccgagt aacctaaaaa gttatttctt
     5401 aaatgaccta agtgtaatgg tttgttggta ttgggcgatg aatattccac caggtaggtt
     5461 tttccgctgg cttgttctgc ttttccaaaa tcttttttca gagctgtagc cagtgttgca
     5521 atccaaagtg aatctgcaag cgaaatgttg aggaatcctt ttacgacctg gtattcggtt
     5581 actataccgg aatattcctt cagatggcgg ccaatcagtt ctgaggtttc ttccggcgat
     5641 ttgcgcagtg cttttgcgaa tggaaaagta acaagcgtta aatcgccggt aaattctttt
     5701 ttcgtttttt gcaatacaac ctggtcgtgc attccaatgc cgggttgaat cgtttcaata
     5761 catgcaatta ttttttcgcg gagaaggatt tcgggtgtca tattgatttt ttgtctgcta
     5821 ctgctacaat gttttttaca atactgaatg cggtttcggc cattttattg attttatcgt
     5881 cgagacatgg attgacttcc acaatctcaa acacactgca cttggacgat gaaaccaatc
     5941 ccgcagtgat ttcatgcgct tcttctgcgg tgaatccacc tgctacaggt gtgcctgttc
     6001 ctcttgatac ttccgggtcc atgctatcaa catcaaagga aacatagata agatcacatg
     6061 ctttcaggta ttccaacgtt tcctgaacaa ccttggctgt acctttttgg cgtacttcgt
     6121 ttaccttaaa gtttctgatg ttattgttta ccataacatg atcttctgcg ggttccgtat
     6181 cgcgtacacc aataaatacc aaatctgtgg ggagtatttt tggactaatt cctccaacat
     6241 tcttcagttt ctcccatccc aatcgggttt cttcctgaat gtcattgatc ttgtcttcga
     6301 ggttatcatt acccaatgca gttgccaaag gcattccgtg aaggtttccc gatggagttg
     6361 tgtacggcga atgaaggtcg gcatgtgcat ctacccaaac cactccaagg cgtttatcgg
     6421 gatgtgcttt tt
//
LOCUS       DAOMYG010000148         6426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4118_length_6426_cov_9.488306, whole genome shotgun sequence.
ACCESSION   DAOMYG010000148 DAOMYG010000000
VERSION     DAOMYG010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4118_length_6426_cov_9.488306"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PK610_06145"
     CDS             complement(<1..991)
                     /locus_tag="PK610_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRJ35547.1"
                     /translation="MRYKLFGKSGLRVSELCLGTMTFGEEWGYGANKEESRKIWNAFL
                     DAGGNFIDTANRYTEGTSEKFIGDFIKEGDRRDELVIATKCTLFTQPNRINDSGNHRK
                     GMVQNLESSLKRLQLDYVDILYVHAWDFTTAEDEVLRALDDLVRSGKVMYIAVSDTPA
                     WMISRCNAIAELKAWSRFAGMQVEYSLITRDAERDLLPLAKYTDMGVTAWAPLAGGAL
                     TGKYLGNNNDPKRLKEGSRRLNEKSMAIAQKVVDIAKEINCPPAHVALNWVRKNSAHI
                     IPIIGSRNVQQFSESLGCFHSTLSDEHMKQLDEVSSIELGFPHDFLTQDGVKMV"
     gene            1218..2204
                     /locus_tag="PK610_06150"
     CDS             1218..2204
                     /locus_tag="PK610_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRJ35548.1"
                     /translation="MSGHQQQNVRLSFLILVLGTGIMAVKFLAWYLTHSNTILTDALE
                     SIVNVLAGSFSLFALWLSSKPRDEEHPYGHGKIEFISAGFEGALILLAGLGILVKSVF
                     NLVHPAHLHDLGTGIGLSVATGGLNYLFGVILVMRGKKSRSLALTANGEHLKSDGYTS
                     AAMVGGLIIVFFTGYVWLDSVVAIIMAMLIVYMGYRIVRRSLAGIMDEADLDVIDEIL
                     EVLNRNRKPEWIDVHNLRVIKYGTTYHIDCHVTQPWYFDLKHSHDSIEELSQIIEREV
                     QAEVEFFIHTDPCIPSSCKICEVQDCKFRSEVVNEKIIWTRTTLLTNQKHGL"
     gene            2201..2614
                     /locus_tag="PK610_06155"
     CDS             2201..2614
                     /locus_tag="PK610_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35549.1"
                     /translation="MIGHLKRKKYFGIVVLIFLSFFSSCKKENIEKACPDFLGEWHSD
                     TLTETATGRRIETYLKVNGDFGEYGFACSVNCTSCECTKLFSGRIRINVDRKLLYFGK
                     ALGGGITRLKIGALPQQDQNGRWWCKINDIVLYKR"
     gene            2617..3189
                     /locus_tag="PK610_06160"
     CDS             2617..3189
                     /locus_tag="PK610_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRJ35550.1"
                     /translation="MRIFLLDNYDSFTWNLEHYLQGLGAEVEVRRNNEVDVEFAASFD
                     RIVLSPGPGLPKDSGILMDVIKRYQNEKPILGVCLGLQAIAIHFGAQLRQLDKVAHGV
                     STSCSITDFSDPLFAGLPPVIEIGRYHSWVVDEKTLPAELIITSEDERGHIMSLRHRK
                     LPIHAVQFHPESVLTPHGKKILRNWMDIRI"
     gene            3429..6089
                     /locus_tag="PK610_06165"
     CDS             3429..6089
                     /locus_tag="PK610_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35551.1"
                     /translation="MLRKIAVLNSKVYGKAVVRVDDCDSLQLVGPNNIGKSTLIYALN
                     FLFIIDGNKMTFSGQRKGDKETIHHYFPTPNQSYTIFEIYKNKAYCILVKRDTEGELE
                     YYHFPAEYRDDLFFREDGAQQRLLKFDEVQERMIEAGLDLVQFKNKSEVFSFVYSRGN
                     DNDAIVWLEENVKSDGLSNNFSKVYRYLINSKLITNKTLKEALIIADNRENEVLNFSQ
                     KNKKDIIDLLRLNDEIKNIKNVKTEFLDFREIVNQYESKSRILSELTYQFNESYVSTL
                     PEMEERIHKKAKEINDLYTEIGEYLRPKEQDLNRKIGQKESDINNQTRLMDDKRLALD
                     EISAIEDMEFLNASLENLEKSRKEIESQITQVESQRYTSNQIKARLDVLAASISEKEN
                     QQRNYNDLLIHKISDDADTRKLLNTVLSERITSLPSGKILDKVKRAAKNLKIFDGEID
                     ISGMSLKEFKSIDEINEELYELRREEEQFKRLLDTTKNSEKVNDELKSIKNEIEEIRE
                     KMRMLKTKPQIEKELSTFRLEVESLRESKEALERELKELTQEILTKEHMLQAMREDRK
                     KTEERVNQLRERKQEVEFLNLKPVECEVTDSLENIYTKMKLHYNDRLELKVTKDKAFD
                     ALRHVLKSSIADESEFIRYFEEEVACLHDKEKSIDGLLQSISTQFANPAYTMMKRFEE
                     FKQFIYTKFNKTLATTRISNIESLQIELLDNKRVLGELAAISSIQEINGQMMLDFDQT
                     ENLRILNNYLDNSRKVHFDELFDIELHLEIKGTIKKVDLANQVESDGTDRMIRLVIIM
                     SIINRLAINEHDNRITLFIDEVATIDKQNRPELVKFCREHNFIPIFAAPDPVPGFSKY
                     YFIYPNKGKININERLNAIYSEPLAEMDNQ"
     gene            6101..>6426
                     /locus_tag="PK610_06170"
     CDS             6101..>6426
                     /locus_tag="PK610_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35552.1"
                     /translation="MSQLKAEPRTILYFIWNHFDLLNELFVEQMEKGYIRDTSLRNIV
                     SKYEFDVRDKLSEYKILRSINDDFEIRPVIFQLIEFVQNEFKPVLPETIEKYGFSILS
                     LFRKIK"
BASE COUNT         1872 a         1327 c         1391 g         1836 t
ORIGIN      
        1 tcaccatttt tactccgtcc tgcgttaaga agtcgtgtgg aaagccgagt tcaatgctgc
       61 tgacctcatc caattgtttc atgtgttcat cggaaagcgt tgaatggaaa caacccagac
      121 tttcactgaa ttgctgaaca tttctcgatc cgataatagg aatgatgtgt gctgaatttt
      181 tacgaaccca attgagcgca acatgtgccg gcggacaatt aatttctttg gcaatatcca
      241 ctactttttg agcaatggcc atgctttttt cattaagtcg gcgtgaacct tcttttaatc
      301 gtttcgggtc gttattattg ccgaggtatt ttcccgttaa tgcgcctccg gctagtggtg
      361 cccatgcggt tactcccata tcggtgtatt ttgccaaggg taaaaggtca cgttcggcat
      421 cgcgtgtaat cagcgaatac tcaacctgca ttcctgcaaa gcgcgaccat gcttttaatt
      481 ctgcaatcgc attgcaacgc gaaatcatcc atgcaggtgt gtcggatact gcgatataca
      541 tcaccttgcc tgaacgtaca agatcgtcca gcgcgcgtaa aacttcatct tcggctgtgg
      601 tgaaatccca agcgtgaacg tagagaatgt ctacataatc gagttgcaac cgttttaaac
      661 tgctttccag attctgcacc attcccttgc ggtggttacc actgtcgttt atgcggttag
      721 gctgtgtaaa cagagtgcat ttggtggcga tgaccaattc atctcttcga tcaccttctt
      781 tgatgaaatc gccaataaat ttttcgctgg ttccttcggt atatcggttg gccgtatcaa
      841 taaaatttcc gcctgcatcc agaaatgcat tccatatttt tcggctttct tccttgtttg
      901 caccatatcc ccactcttca ccaaatgtca ttgtacctaa acataattcc gaaacacgaa
      961 gtccgctttt accaaataat ttgtaacgca tacacgattt tttttggtaa gatattgcat
     1021 tttgggattt aatgtttgtc ggttgaaaaa aaagagggga aatccacctg agggatgccg
     1081 tagtccattg ttttgttaag actttcctct aattaatgta tcctgcagac cattggtaat
     1141 gctgacgtaa aacaaaatat cccggttaaa aattcagtgt gcgcatttca gtgcttttaa
     1201 tgctttatct ttactttatg agtggccacc agcagcagaa tgtccgactt tccttcctga
     1261 ttttagtatt gggaaccggt attatggcgg tgaaatttct ggcatggtac cttacccaca
     1321 gtaataccat cctcactgat gctctggaaa gtattgttaa tgttttggca ggatcttttt
     1381 ctttgtttgc attatggctt tcgtccaaac cacgcgatga ggagcatccc tatggacacg
     1441 gtaaaattga attcatttcg gcaggatttg aaggcgcatt gattttgctt gcaggattgg
     1501 gtattctggt taagtctgtt ttcaatcttg tacatcctgc acatttgcat gatctgggaa
     1561 cgggtattgg attgtcggta gcaaccggag gattgaatta tttgtttgga gttattctgg
     1621 taatgcgcgg aaaaaaatcc cgttcgcttg cactaactgc caatggtgaa cacctcaaaa
     1681 gtgatggata tacatctgct gccatggtcg ggggattgat cattgtcttt tttacgggat
     1741 atgtttggct tgattcagtt gttgctatta ttatggcaat gttaattgtt tacatgggtt
     1801 accgcattgt acggcgttca ctggcaggaa taatggatga agctgatctg gatgtgattg
     1861 atgaaattct cgaagtattg aacaggaacc gaaaaccgga atggatcgat gtacataatt
     1921 tacgtgtcat caaatacgga acgacctacc atatcgattg ccatgtaacc caaccctggt
     1981 atttcgattt aaaacattcg cacgatagca tcgaggaatt atcgcagatt attgaacggg
     2041 aagttcaggc ggaagttgaa ttttttattc atactgatcc ttgtatccct tcatcctgca
     2101 aaatttgtga agtgcaggat tgtaaattca gaagcgaggt ggtaaatgaa aaaataattt
     2161 ggacaagaac tactttgctg accaaccaaa aacacggttt atgattggtc atttaaaacg
     2221 aaaaaaatat tttggaattg tagtcctcat ttttctgagt tttttctctt cctgcaaaaa
     2281 agaaaatatt gagaaggcct gtcccgattt tttaggtgaa tggcattccg acacactcac
     2341 agaaacagcc accgggagac gtattgaaac ctacctaaaa gttaacggtg attttggtga
     2401 atatgggttt gcctgctctg ttaattgcac ttcctgcgaa tgcacgaaat tgttttcagg
     2461 tcgtattcgt atcaatgtcg atcgaaaatt gctttacttt ggcaaggcct tgggtggcgg
     2521 aattacccgg ttaaaaatcg gagcattgcc acagcaggat caaaatggaa gatggtggtg
     2581 taaaatcaat gatattgtgc tttataagcg ataaacatgc gaatattctt acttgataat
     2641 tacgatagtt ttacctggaa tcttgagcac taccttcaag gactcggtgc tgaagttgaa
     2701 gtgcgtcgta acaatgaagt agatgtcgaa ttcgcagcat cattcgatag gattgttctc
     2761 tcacccggac caggccttcc caaggattcg gggatattaa tggatgtgat aaaacgctat
     2821 caaaatgaaa aaccaattct gggtgtttgt cttggactac aggcaatcgc cattcatttt
     2881 ggagcacaac ttcgtcagct ggataaagtt gcgcatggag tctctacttc ctgttctatt
     2941 accgatttca gcgatcctct ttttgcagga ctccctcctg tgattgagat tggtcgttac
     3001 catagctggg tagtggatga aaaaacactg cctgctgaac tgatcattac atccgaagat
     3061 gagcgtggac atatcatgtc tttgcgtcac cggaaattac ccattcatgc tgtgcagttt
     3121 catcctgaat ctgtactcac ccctcatgga aaaaagatcc tccgaaactg gatggatatc
     3181 cggatctgat tcgagcctca aagtcctttc acattgttca tttgttgata aaagaaaggc
     3241 aggtattcaa caatttttgc ccttttttat tcccatcatt tttctttttc cacagtctta
     3301 atcctttgct gataaaggga tacgggttta ttaacagtcg gagctccggt gttgagaaat
     3361 ccttcatgtt ctcaaccgtt ccgggggtgg ggcttgccta tctttgttag aatcaaattt
     3421 caagcgaaat gctacgtaaa atcgcagttc ttaattcaaa ggtttacgga aaagctgtag
     3481 ttcgggtcga cgattgcgac tcattacaac ttgtcggacc aaacaacatt ggtaagagta
     3541 cgcttatcta tgcgctgaac tttctgttta ttatcgacgg aaataaaatg accttctcgg
     3601 gtcagcgtaa gggcgataag gagacgatcc accactattt tccgactccc aaccagagtt
     3661 ataccatctt tgaaatttac aagaacaaag cctattgtat tctggtgaaa cgcgataccg
     3721 aaggtgaact ggaatattat catttccctg ccgaataccg cgacgatctt ttcttccgcg
     3781 aagatggtgc gcaacagcga ttgctgaagt ttgatgaggt tcaggaacga atgatcgaag
     3841 caggtcttga cctggtgcag ttcaaaaaca aatctgaagt tttcagtttc gtttacagtc
     3901 gtggtaacga caacgacgct attgtttggc tggaagaaaa tgtgaagtcc gatggtcttt
     3961 ccaataactt ctccaaagtt taccgatacc tgatcaattc caagctgatc accaacaaaa
     4021 ccttgaagga agcactcatc attgcggata accgtgaaaa cgaagtgctc aatttctctc
     4081 agaagaacaa aaaggacatt atcgatcttc ttcgtttaaa tgatgagatt aaaaatatta
     4141 agaacgtaaa aacagaattc ctcgattttc gcgagatcgt aaaccaatac gaatcgaaat
     4201 caagaattct aagtgagctg acctatcagt tcaacgagtc ttatgtttct actcttccgg
     4261 agatggaaga acgcattcat aagaaggcca aggaaatcaa tgatctttat actgagatcg
     4321 gcgaatacct gcgtccgaaa gaacaggatc ttaaccgtaa gatcggtcag aaagaaagcg
     4381 acatcaacaa tcagacacgt ctgatggatg ataaacgtct tgctctggat gagatcagtg
     4441 ctattgagga tatggaattc ctgaacgctt cgctggagaa tctggagaag agtcgcaagg
     4501 aaatcgaatc gcagatcaca caggtagaga gtcagcgtta tacatccaac cagatcaaag
     4561 cgcgtctgga tgtgcttgct gcatcaatca gcgagaaaga aaatcagcag cgtaactata
     4621 acgatctgct cattcataag atcagtgatg atgccgatac acgcaaatta ctgaatacgg
     4681 tattatcgga acgcatcaca tcgcttcctt caggaaaaat tctggataag gtaaagcgtg
     4741 cagcaaagaa tcttaagatt tttgatgggg agatcgatat cagcgggatg tcactgaagg
     4801 aatttaaatc gattgatgag atcaatgaag aattgtacga attacgtcgt gaagaagagc
     4861 agttcaaacg tcttcttgat accaccaaga attctgaaaa agttaacgat gaattgaaaa
     4921 gcatcaaaaa cgagatcgaa gagatccgtg aaaagatgcg tatgctcaag accaagccgc
     4981 agatcgaaaa ggaattatct acattccgcc tggaagtaga aagtctgcgc gaaagtaagg
     5041 aagcacttga gcgtgaattg aaagagctca cacaggaaat tcttacgaag gaacacatgt
     5101 tacaggctat gcgtgaagac cgcaaaaaga ccgaagaacg tgtgaatcaa ttgcgtgagc
     5161 gcaagcagga agtggaattc ctcaacctta agccggttga atgtgaagtg accgattcgc
     5221 tggagaacat ttataccaag atgaagttgc attacaacga tcgtcttgaa ttaaaagtga
     5281 ccaaggataa agcgtttgat gccttgcgtc atgtacttaa gagttcaatt gccgatgaat
     5341 ctgaattcat ccgttatttc gaagaagagg ttgcttgttt gcatgataaa gagaaatcga
     5401 tcgacggact cctccaatca atttctaccc agtttgccaa cccggcgtac acaatgatga
     5461 agcgttttga ggaattcaaa caattcatct ataccaaatt caacaagaca ctcgcaacaa
     5521 cacgtatttc gaatatcgaa tcgttgcaga tcgagttact ggataacaaa agggtcttgg
     5581 gtgaacttgc cgcaatcagt tcaattcagg agatcaacgg tcagatgatg cttgatttcg
     5641 atcagacaga aaacctgcgc atcctgaaca attacctcga taattcacgt aaagtgcatt
     5701 ttgatgaatt gttcgacatc gaattgcatc tggagatcaa ggggaccatt aagaaggtgg
     5761 atcttgccaa tcaggtggaa tcagatggta ccgaccgtat gattcgtctg gtgatcatca
     5821 tgtcgatcat taatcgtctt gccattaacg agcacgataa ccggatcaca ttgttcatcg
     5881 acgaggttgc aaccattgat aagcaaaacc gtccggagct ggtaaaattc tgtcgcgaac
     5941 acaacttcat tccgattttc gccgccccgg atcctgtgcc cggattcagt aagtattatt
     6001 tcatctaccc caacaagggc aagatcaaca tcaacgagcg actcaacgcc atttacagcg
     6061 agccgcttgc agaaatggat aaccaataag gacccgcgcc atgagccaac tcaaagccga
     6121 accgcggacc atcctctact ttatctggaa tcacttcgat ctccttaacg agctctttgt
     6181 ggaacagatg gagaaaggtt atatccgcga cacatcattg cgtaacatcg tttccaaata
     6241 cgaattcgat gtgcgcgata aattgagcga gtacaaaata cttcgttcaa tcaacgacga
     6301 tttcgagatt cgtccggtta tcttccagct gatcgagttt gtacaaaacg aattcaaacc
     6361 tgtacttccc gaaacaatcg agaaatatgg attttcgatt ttgtcgcttt tccgcaagat
     6421 caaaga
//
LOCUS       DAOMYG010000149         6289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4219_length_6289_cov_9.252807, whole genome shotgun sequence.
ACCESSION   DAOMYG010000149 DAOMYG010000000
VERSION     DAOMYG010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4219_length_6289_cov_9.252807"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PK610_06175"
     CDS             <1..858
                     /locus_tag="PK610_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRJ35553.1"
                     /translation="ELARIIHDTTLPKGAFSVLPCDRNTGNALVTSNDIQVLSFTGSP
                     SVGWKMKDTAGKKKTVLELGGNAGVFIAADANIDHAVERCLVGAFAYSGQICIHTQRI
                     FVEDKVFEEFVEKFMPRVEMLKTGDPSQKDTELSSMIDEENAIRVDHWVKEAIAAGAK
                     MLCGGNRNGNFYEATILTGTTASMKVHAEEIFGPVVCVEKVNNKEDGIRQLNNTRFGL
                     QAGLFTNDHAFIQKAFKKLEVGGVLVNEVPTVRFDHMPYGGVKDSGQGREGVKYAMLD
                     FLEPKILVL"
     gene            complement(880..1215)
                     /locus_tag="PK610_06180"
     CDS             complement(880..1215)
                     /locus_tag="PK610_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35554.1"
                     /translation="MNRASITAFVFASLLFGFLYIMFTNKTLVFKTFEVQMETEDYFS
                     GEKKTAPIKSNLGCVLDGTCILTFNGWMKVLVFFAGLPLMMFFFVRWRINKKIAKALE
                     KQQQGTVDA"
     gene            1313..3715
                     /locus_tag="PK610_06185"
     CDS             1313..3715
                     /locus_tag="PK610_06185"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HRJ35555.1"
                     /translation="MRIIITLLLGFWTSNLLHSQLIVTTTQTPLQLVQNTLVGGGVAV
                     SNVTFNGVPGVNPHPQIGLFNGVNTTLNMNTGLILASGQVTNAIGPNTSGSIATMYGA
                     FSSDPDLNAISNFAVFDAAILEFDFVPSGDTVKFSFIFGSDEYIEYVGSGINDAFGIF
                     LSGPGISGPFSNGAVNMATVPGTSTPISIDNISPFTNAMYYVDNGNGFTAPFNSNPMY
                     VQYDGLTVKITAAYPVQCGQTYHIKFAIGDGADDFIDSGVFIEGGSFSSNAPSVSLIT
                     PTLPNATQGVVYEGCLIGSTVDFTFVRPDVGSNDTVFFNLSGNAINGVDYSLISPSYL
                     VFPPGQDSVTLTITIPNDFVVEGVDTLIISVLGFTACGSQTTNSAALYIHDPYNVLPF
                     AGNDTVYNCPGQTFSYNGVVLNGNPPYNYSWSNSVTGQSINYTITQLGGDTLILSVID
                     GCGYSGNDTIFYSQVPPAPLFANAGPDTTLLCAGQTVLLQGSHNGGSGIPVTYSWGSA
                     GNTQNILVQPLSTTMYVLSVTNTCNQFDLDTVFVIVPPYVPLSVIVSDTVVAVNCAGD
                     LATFSGQIISGGNPPYSYFWSNMVNDTITTISVLGDTLIALTAVDGCGLDTTLFFRAE
                     IAGGNFALDVSSGRHCLNSDSTALVPFTINGGVAPYSFSWTTPVGVTSITVDVPNQNF
                     LIADAQTGAYVYTITDGCGTVITDTSSVIMISCALDIPNVISPNGDGLNDVFFIDGLQ
                     FHPNSVLIVYNRWGQQVYYDGNYQNNWDGNGLSAGTYYYIIELTDGTVPSQYHGTLSI
                     LY"
     gene            complement(3789..5039)
                     /locus_tag="PK610_06190"
     CDS             complement(3789..5039)
                     /locus_tag="PK610_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007093112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRJ35556.1"
                     /translation="MALDIFEKIKLNRGPLGKHSKESHGYFTFPKLEGPIGNKMIFRG
                     KEVLVWSLNNYLGLANHPEVREADAKAAADFGLAYPMGARMMSGNSNYHEQLENEISA
                     FMKREDTILLNFGYQGMVSAIDSLVDRNDVIVYDSESHACILDGMRLHFGKRFVFQHN
                     DMDSLEKQLARAVKVAEEKDGGVLVITEGVFGMAGDQGKLKEIVALKKKYPFRLFVDD
                     AHGFGTMGPTGAGTDEEQGCQEGVDIYFGTFAKSMSLIGGFISSTENIVEYLRYNMRS
                     QIFAKALPMPIVIGALKRLELLRSRPELREKLWTIANALQSGLKEAGFNIGNTNTMVT
                     PVFLTGTLGEATNLTFDLRENHSIFCSIVVYPVVPKDVIMLRLIPTAAHTLEDVSYTI
                     ETFKKVAVRLKKGEYHSDKIVNVN"
     gene            5221..6042
                     /gene="bamD"
                     /locus_tag="PK610_06195"
     CDS             5221..6042
                     /gene="bamD"
                     /locus_tag="PK610_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRJ35557.1"
                     /translation="MKLKHSLITGLISILLLSACSEYGKVLKSSDSGLKLTKAYEYFE
                     KKDFDRALPLFEDLVGSGVYRGTEKAERVYYSYAWCHYHLKEFYLAAYYFKMFSKTYP
                     NSVYAEECLFMSAICNVKNSPNWSLDQTETIDAINELQLFLNRFPESRRRDTCNIIMD
                     QMYNKLETKAFENAYLYYKIENYKAASIALTSMVDEYPNSRYVERAMYLIVKSDYLLA
                     MNSIESKKLERFEQTLKSYTNFVSLFPQSTYRKELEGYQQNAAKLAELLKKKTGS"
     gene            6039..>6289
                     /locus_tag="PK610_06200"
     CDS             6039..>6289
                     /locus_tag="PK610_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004916340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRJ35558.1"
                     /translation="MSYKNSTAERTTVTRDLDKLEVRTGNLYEAIVVIGKRANQISVE
                     MKEELTSKLQEFATHTDNLEEVFENREQIEISRHYEKLP"
BASE COUNT         1725 a         1324 c         1401 g         1839 t
ORIGIN      
        1 gagctcgcac gaatcattca cgataccacg cttcccaaag gagcgttttc tgtgctgcct
       61 tgcgacagga ataccggaaa tgcattggtg acttccaatg atattcaggt tttatcattt
      121 acgggatcgc cttccgttgg gtggaaaatg aaagatacag caggtaaaaa gaaaacagtg
      181 cttgaattgg gaggtaacgc cggtgtattt attgctgccg acgccaatat tgatcacgct
      241 gttgaacgtt gtcttgttgg tgcctttgcg tatagtggtc agatttgcat ccacacgcag
      301 cgaatttttg tggaggataa agtattcgaa gaattcgtgg aaaagttcat gccgcgcgtt
      361 gaaatgctca aaacgggcga tccttcgcaa aaggatactg agctttcttc aatgattgat
      421 gaggaaaacg caattcgggt cgatcattgg gtaaaagagg cgatcgccgc cggtgcgaaa
      481 atgttgtgtg gtggaaacag aaacggaaat ttttatgaag caaccatttt aaccgggacc
      541 accgcatcca tgaaagtgca tgcagaagaa atttttggtc cggttgtttg tgttgaaaaa
      601 gtaaacaaca aagaagacgg aattcgtcag ctcaacaata cccgatttgg attacaggca
      661 ggattattta ccaacgacca tgcatttatt caaaaagcat ttaagaaact ggaagtagga
      721 ggagttctgg taaacgaagt tcccacggtg cgttttgatc acatgcctta cggtggagta
      781 aaggatagtg gtcagggcag agaaggagta aagtatgcaa tgctcgattt cctcgaacca
      841 aaaattttgg ttttgtaaat gcaatcgctg ttttaatcct taagcatcaa cagtgccttg
      901 ttgttgtttt tccaatgctt ttgcgatctt cttattgatt ctccagcgga caaaaaagaa
      961 catcattaaa ggtaagcctg caaaaaacac gagtactttc atccatccgt tgaaggtaag
     1021 gatgcaggtg ccatcaagta cacaacccag attggattta atgggtgctg tttttttctc
     1081 tccggaaaaa taatcttccg tttccatttg cacttcaaac gttttaaaga caagcgtttt
     1141 gttggtgaac attatataaa gaaacccgaa taaaaggctt gcaaatacaa atgcggtgat
     1201 tgaggctctg ttcatggctg gaaatggttt acaggactaa tttattcaca ttttcggagt
     1261 ctgtgcaacc ttcagctgct ttttggtgtc tgattaatga aaacagctac ttatgcgtat
     1321 tattattact cttttattgg ggttttggac ctcaaatctt ctacatagtc aattgattgt
     1381 aaccacaact caaacgccgc tgcaattggt tcaaaatacg cttgttggtg gtggagtggc
     1441 ggtttcaaat gtcacgttta acggagttcc cggtgtaaac cctcatcccc aaattggctt
     1501 gtttaatgga gtaaatacta cattaaatat gaacaccgga ttgatactcg cttccggtca
     1561 ggttacaaat gcgattggtc caaatacaag tggaagtatt gcaaccatgt atggtgcctt
     1621 tagctcagat cccgatctga atgcaatttc caattttgca gtatttgatg ctgcaattct
     1681 cgaatttgat ttcgttccct ccggggatac cgtaaaattt agtttcattt tcggaagtga
     1741 tgaatacatc gaatatgtag gaagcggaat caatgatgca ttcggaatct ttttgagcgg
     1801 acccggtatt tcaggtccat tttctaatgg tgcagtgaat atggcaacag ttcccggaac
     1861 aagcacaccc atttcaatcg ataatatcag tccgtttaca aatgctatgt attatgtgga
     1921 taacggaaac ggattcacag ctcctttcaa ttcaaatccg atgtatgtgc aatatgacgg
     1981 acttactgtt aaaattactg cagcgtatcc tgttcagtgc ggacaaacct atcatataaa
     2041 atttgctata ggtgatggtg cagatgattt tattgattcc ggtgtgttta ttgaaggtgg
     2101 aagcttttct tcgaatgcac cgagcgtttc actgattact ccaaccttgc ccaatgccac
     2161 tcagggtgtt gtatacgaag gttgtcttat cggttctacc gttgatttca ccttcgttcg
     2221 gcctgatgtt ggctctaacg ataccgtatt ctttaacctg agtggtaatg cgatcaatgg
     2281 tgtggattat tcgctaatct ctccttctta tctggttttt cctcccggac aggatagcgt
     2341 tacgcttacc attaccattc ccaacgattt tgttgttgaa ggtgtagata ctttaatcat
     2401 tagtgtactc ggatttacag cctgcggttc acaaacaacg aattcggctg cgctttatat
     2461 tcatgatcct tacaacgtac ttccatttgc aggaaatgat acggtgtata attgtcccgg
     2521 acaaacattc tcctacaatg gcgttgtgtt aaatggaaat ccgccctaca attattcctg
     2581 gagtaattcg gttacaggtc agtcgatcaa ttataccatc acccaattgg gaggcgacac
     2641 gctgattctt tctgtaatcg atggttgcgg gtattccgga aacgatacta ttttctattc
     2701 tcaggtacca ccggctcctt tgtttgccaa tgcgggtccg gacaccactt tgctttgtgc
     2761 aggacaaacc gttctcttgc agggaagtca taatggtggt tctggcatcc cggtaaccta
     2821 ttcatggggt tctgcaggaa acacacagaa tattcttgtg caaccgcttt caactaccat
     2881 gtatgtattg tcggttacca acacctgcaa tcaatttgat ctggatactg tttttgtaat
     2941 tgttccaccc tatgttccgc ttagcgtaat tgtttccgat actgttgttg ccgttaattg
     3001 tgcgggtgat ttggctactt tttccggaca aatcatttca ggtggtaatc cgccttacag
     3061 ttatttctgg agcaatatgg tgaatgatac catcaccacc atttccgttt taggcgatac
     3121 attaattgcg ttaacagcag tggacggttg cggtttggat accactttgt ttttccgggc
     3181 agagatcgca ggaggaaatt ttgcgctgga tgtaagttca ggtcgtcatt gcctcaatag
     3241 cgacagtacc gcactagttc catttaccat caatgggggt gtagcaccct acagtttcag
     3301 ctggacaact ccggttggag taacaagtat tactgtggat gtaccaaatc agaattttct
     3361 tattgcggat gcacagacag gcgcttatgt ttataccata accgatggtt gtggaacggt
     3421 aattacagat acttcttccg taatcatgat cagttgtgcg ctcgatattc ccaatgtgat
     3481 ttctcccaat ggcgatggtt taaatgatgt gttctttatt gatggattgc agtttcatcc
     3541 caacagtgtt cttattgttt acaacagatg gggacagcaa gtatactacg atggaaacta
     3601 ccagaacaac tgggatggaa acggattatc tgcaggaaca tattattaca tcatagaact
     3661 cactgacgga acagttccat cacaatacca cggaacgctt tctattctgt attgattgca
     3721 ttaagtctta attctaaata aaaaagccct gacgaattcg tcagggcttt ttttatggaa
     3781 tgcaaatatt agttgacgtt tacgatctta tcggagtggt attctccttt tttaagtcta
     3841 acggcaactt ttttgaatgt ttcaatggtg tagctaacat cttcgagcgt gtgtgcagca
     3901 gttggaatta aacgcagcat gatcacatct ttcggcacaa ccggatacac aacgatcgaa
     3961 cagaaaatac tgtggttctc acgaagatca aatgtaaggt tggttgcttc acccaatgtt
     4021 cctgtaagga aaaccggagt aaccattgta ttggtatttc cgatgttaaa cccggcttct
     4081 ttcagtccgc tttgcagtgc atttgcgatg gtccacaatt tctcgcgcaa ttcaggacga
     4141 gagcggagca gttcaagacg ctttaatgca ccgataacga tgggcatagg taaggccttt
     4201 gcgaaaatct gcgagcgcat attgtaacgc aggtattcaa cgatgttttc ggtggaggaa
     4261 atgaatccgc cgatcagcga cattgatttt gcgaaagtgc caaagtagat atccacgcct
     4321 tcctgacagc cttgttcttc gtcggtaccg gcaccggttg gacccattgt tccaaaacca
     4381 tgagcatcgt cgacaaagag acggaaagga tattttttct tgagtgcaac gatttccttg
     4441 agtttaccct ggtcgccagc cataccgaaa acaccttcgg taattaccag cacgccacca
     4501 tctttttctt cagcaacttt aactgcacgc gccaattgct tttcgaggct gtccatgtcg
     4561 ttgtgctgga aaacgaaacg ctttccgaaa tgcagacgca ttccatcgag gatacaggca
     4621 tgcgattcgc tgtcgtaaac aattacatcg ttacggtcaa ccagactatc gattgcagaa
     4681 accattccct gataaccgaa gttgagcagg atggtatctt cacgcttcat gaaagcggag
     4741 atttcgtttt cgagttgttc gtgataattg gagttaccgc tcatcatacg cgcacccatc
     4801 gggtaggcaa gcccgaaatc ggcagcagct ttggcgtcgg cttcacgtac ttcggggtgg
     4861 ttagccaggc cgaggtagtt gttcaggctc caaacgagca cttctttccc acggaagatc
     4921 attttgttac caatgggacc ttccagtttt ggaaaggtga aataaccgtg cgactctttc
     4981 gagtgttttc caagtggacc gcggttgagt ttgatcttct caaaaatatc caaagcaata
     5041 gagattaagg gttaatactg ttcgaggcac ggattttttt tccgaaccgg aatacaaaat
     5101 taccacgatg ctgcgttaat ggcaatagct ggagaataaa ttatcaacag ccctggaaac
     5161 aggattcccc caaccgtttt cagacatttg atttggcatt attatctata tttgcacccc
     5221 atgaaattga agcactccct cattaccgga ctcattagca tccttttgct ttcggcttgt
     5281 tccgaatacg gaaaggtcct taagagctcc gattcgggat tgaagctgac caaagcctac
     5341 gaatatttcg agaaaaagga cttcgaccgt gccttgccgc tgtttgaaga cctggtggga
     5401 agcggtgttt atcggggtac tgaaaaggct gagcgcgtgt attatagcta cgcatggtgc
     5461 cattatcacc ttaaggaatt ttatttggcg gcctattact tcaagatgtt ttccaagacc
     5521 tatccaaaca gtgtatatgc tgaggagtgt ctgtttatga gcgccatctg taacgttaag
     5581 aattcaccca actggtcgct cgatcagacc gaaaccatcg atgccatcaa tgaactgcag
     5641 ctcttcctga accgttttcc cgaatcgcgt cgccgggata cctgcaatat tatcatggat
     5701 caaatgtaca ataagctgga aaccaaagct ttcgaaaacg cgtacttgta ttacaagatc
     5761 gaaaactaca aggcagcatc cattgcactc acgagtatgg tggatgagta tccgaacagc
     5821 cgatacgttg agcgtgctat gtatttgatc gtcaaatcag attacctgct ggcgatgaat
     5881 tcgatcgaat caaaaaaact ggagcgtttt gagcaaacat taaaatctta tactaatttt
     5941 gtatcccttt tcccgcagag tacataccgt aaagagctgg aagggtatca acaaaatgct
     6001 gcgaaattgg cagagttatt aaagaaaaag acaggatcat gagctacaaa aattcaactg
     6061 cagagcgtac aacagtaaca cgcgacctcg acaaactcga agtgcgcacc ggtaatctgt
     6121 atgaagcgat cgttgtaatt ggcaagcgtg caaatcagat ctctgttgag atgaaggaag
     6181 aattgacttc caaattgcag gagttcgcaa cacacaccga taacctggaa gaagttttcg
     6241 aaaaccgcga gcagatcgag atctctcgcc attatgagaa gcttcccaa
//
LOCUS       DAOMYG010000150         6285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4223_length_6285_cov_8.106902, whole genome shotgun sequence.
ACCESSION   DAOMYG010000150 DAOMYG010000000
VERSION     DAOMYG010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4223_length_6285_cov_8.106902"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="PK610_06205"
     CDS             complement(<1..1012)
                     /locus_tag="PK610_06205"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRJ35559.1"
                     /translation="MTDQLDREEERLEDENGEELFEHHRIKADKGQSLLRIDKFLFDR
                     LPNTSRNRIQLAAKNGNILVNNKAVKPNYKVKPFDDISIVLPYPVREIELIPQNIPIH
                     IFYEDEDLAVINKPAGLVTHPGYGNYSGTLVNAMMYHFSEISSMRKEGNDVRPGLVHR
                     LDKNTSGIMVVAKTDEALTHLAKQFFDRTTERTYYALVWGDVKEEEGTIVGHIGRSLQ
                     DRKVFRVYEDGSIGKHAVTHYSVVERLGYVTLVKCKLETGRTHQIRVHMKYIGHPLFN
                     DNEYGGDKILKGTTFAKYTAFVENCFSLIPGQALHARTLGFIHPRKNEWMQFDSELPE
                     GFA"
     gene            complement(1009..3246)
                     /locus_tag="PK610_06210"
     CDS             complement(1009..3246)
                     /locus_tag="PK610_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ35560.1"
                     /translation="MQGQKTKALILICTLVQLCTYSYAQNEALSPLSENFDLTSTKKE
                     VRMFPSLFVYEIDTILLPFLDDFSRDRFKPYNAQAGDANVTSQTFFRLYDAAGTVLLH
                     DSVKYMRNPTYYITYDSIAGVLVTTINPLDSVLITVNDLTSYPITGVQEYVYPRYSII
                     DTLWQPSSPDTIYIQNVDLVQDSVYVNFVSSAGDNSLWVDRFAYLNNRFPIGPPTIGV
                     ATLDGLNDEGYPYDFVNTTLTGVCDYLTSKPIDLDIKPNTFPYTAADSVYLSFFYQQT
                     GRGNQPEVGDSLILEFWSPSANDWFRQWASPGALAFNDFRQVLVPVLQNMFFENGFRF
                     RFRNIGNLSGSLDHWHIDYVYMAANRNRNDTIRQDVAYQYDALSLLKEFSRMPWEHYK
                     LNPTFYMADTMSLYQRNNDNVARIIVAQNMVVKYQGVTQGTITAPNIPSVAAVSNFSS
                     LFDINASGYSFDPLVADSCAVEFDITASFNPGSNFQLNDTLHFKQVFNDEYAYDDGTA
                     EAAYGPQGANAYLAYKFEIQQADTLRSIRIHFSPSVNDVSNKLFILHVWDASGPGITP
                     GSVIYQNTFLQTPEYVMENNGFYEYPLAQPVVVNGTYFIGWKQLDPDKLNIGFDLNTN
                     RQSKIYYRTAASWIQTGFQGALMMRPVFDRCIGDVTSVEDFSPEEMQLRLFPNPATDE
                     VVIDSDLNNGTIEIYDLGGRLISTTITTGTVRLGVSLLQNGVYLVAFINDQGARAVRK
                     LIIQR"
     gene            complement(3260..4048)
                     /locus_tag="PK610_06215"
     CDS             complement(3260..4048)
                     /locus_tag="PK610_06215"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRJ35561.1"
                     /translation="MRTLGNIGRFLISRQFLFHFGAGLLTMILLFYCTVWYLKSYTHH
                     GKENYITLPDFIGMPLEEIDGKLTSLGLRYQIDSVSTDKYPRGTVIRQDPGPTDSTGQ
                     FVKEGRTVYLTVVSKSPLMVSMPDLVYKSKKHAEGILKIVGLKVKYTYKPYPDCKDCV
                     IEQKYKARPISKGDKVEKGSTIELVLGQGRGGGTETVPDLTGKTIDEANSSLNRVALS
                     LYAASCEGCVTRKDSVSARIYRQSPGAGGEASSGSEVTVWLRND"
     gene            4099..5175
                     /locus_tag="PK610_06220"
     CDS             4099..5175
                     /locus_tag="PK610_06220"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRJ35562.1"
                     /translation="MSKRGKITNKRMGKQSAFGEYPRWEAGYFCKKYSMKTRIAVLSG
                     GYSHEAQISRKSASTVMRHLPTDRYDLMQIDISEEGWFALSGDEKIPVDKNDFSVIVN
                     GKKHQFDAAFIVIHGTPGEDGKLQGYFDMLKLPYTTSGVLTSALSFNKFACNTLLSQM
                     GIRCSKSLIIRKGDPISSENIAMTIGFPMFIKPSDGGSSFGVSKVKQAEEIEKAISTA
                     LEHGTEALIEAFVNGTEVTCGVFSVNGKVTVLHPTEIVTENEFFDFDAKYQGKSKEIT
                     PARLSEEVTNLVRATTAEVYRLLNLRGMARVDFIIQENVPFMIEVNTVPGLSDASILP
                     QQARHAGIELSTLFSSVLEDALKR"
     gene            complement(5165..5746)
                     /locus_tag="PK610_06225"
     CDS             complement(5165..5746)
                     /locus_tag="PK610_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010520944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HRJ35563.1"
                     /translation="MKIVFASQNQHKIDEIAALLDDSIQISGLDEYNLKEEIPETGST
                     LTENALQKARFVSGRFGVNCFADDTGLEVSALNGAPGVYSARYAGEQKSAEANMKKLL
                     LELQGKVDRSACFKTVIALLLDGKEYLFEGVMNGNIITERRGSGGFGYDSLFVPEGSS
                     LTFAEMSAQEKNKCSHRAKAVEKLIAFLNELNA"
     gene            5863..>6285
                     /locus_tag="PK610_06230"
     CDS             5863..>6285
                     /locus_tag="PK610_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRJ35564.1"
                     /translation="MIIGAQKSGTTSLLRYLGEHPQIESHKANEFSYFYSDEEYESGW
                     AKAEEYYFGKKNSDKNFVVAKHANLYSSAKAIERLYEQFPQCKLVLLLRNPVSRSYSS
                     YLMEKMYDRVDYEFDELPELLEKKDGRQLESWQEEAFIG"
BASE COUNT         1875 a         1378 c         1286 g         1746 t
ORIGIN      
        1 ccgcaaaacc ttccggtaat tccgaatcaa actgcatcca ctcatttttt cgaggatgaa
       61 tgaatccaag tgtgcgtgca tgcagcgcct gaccaggaat aagcgaaaaa cagttttcca
      121 caaatgctgt gtatttcgca aatgttgtcc cttttaaaat tttatcgcct ccgtattcgt
      181 tgtcattaaa caaagggtga ccaatatact tcatatgtac ccgaatctga tgtgttctac
      241 cggtttcgag tttgcatttc accagtgtaa catatcccaa tcgttcaaca actgaataat
      301 gcgttaccgc atgctttcca atagaaccat cttcgtaaac ccgaaaaact tttcggtctt
      361 gcaatgatct gccgatatgt ccaacaattg ttccttcttc ctccttcaca tcaccccaaa
      421 ccagcgcata ataagttctc tcggttgtac gatcgaaaaa ttgttttgca agatgagtca
      481 gcgcttcatc cgttttcgca acaaccatta ttccggaagt gtttttatcc aggcgatgca
      541 ccagtcctgg acgaacatca tttccttctt tccgcatgct gctgatttca ctgaaatggt
      601 acatcatggc attcaccaat gtgcccgaat aatttccata ccccggatgt gttactaaac
      661 cggcaggttt atttatcact gcaagatctt cgtcctcata aaaaatatga atcggaatat
      721 tctgcggaat caattcaatt tcccgaaccg gataagggag tactattgag atatcatcga
      781 agggtttaac cttataattc ggcttcaccg ctttattgtt caccaggata ttcccgtttt
      841 ttgctgcgag ctgaatcctg tttctcgaag tattgggaag gcggtcaaaa agaaatttat
      901 caatgcgaag aagtgattga cctttgtccg cttttattcg gtgatgttcg aataactctt
      961 caccattttc atcttccaac cgttcctctt cgcggtctaa ttgatccgtc atcgctgaat
     1021 aatcagttta cgaactgctc gagctccctg atcatttata aaggcaacca ggtacactcc
     1081 attttgcaat aaagaaacac ccaaacgaac agttcctgta gtgatggtag tagaaattaa
     1141 tctgcctccc aaatcgtaga tttcaatcgt accgttattc agatctgaat ctatcaccac
     1201 ttcatcggtg gcaggatttg gaaacaaacg gagctgcatt tcttccggag aaaaatcttc
     1261 taccgatgtt acgtcgccaa tacacctgtc aaacaccggg cgcatcatca aagcaccctg
     1321 aaaaccggtt tggatccacg aagccgcagt tcggtaatag atctttgatt gtcggttggt
     1381 gtttaaatca aaaccgatat tcaatttatc aggatcaagt tgtttccatc cgataaaata
     1441 agttccattt acaacaacgg gctgggccaa gggatattca taaaacccgt tgttctccat
     1501 cacatactcg ggtgtttgca aaaaagtatt ctggtatata acacttcccg gagttattcc
     1561 gggtccgctt gcatcccaaa catggagaat aaaaagtttg ttggaaacat catttacact
     1621 tggtgaaaaa tgaatgcgga tagaacgcaa agtatcggct tgctgaattt caaacttata
     1681 cgctaaatac gcattcgcac cctgtggacc ataagcagcc tctgcagtac catcatcata
     1741 agcgtattcg tcgttaaaaa cctgtttgaa atgcagggta tcgtttaact gaaaattgct
     1801 tcccggatta aatgatgcgg tgatatcaaa ctcaactgca catgaatcgg caaccaaagg
     1861 atcgaacgaa taacctgatg cattgatatc gaaaagcgaa ctgaaatttg atactgctgc
     1921 aacacttgga atatttggcg cagtgattgt tccctgagtt actccctggt attttacaac
     1981 catattctgc gcaacaataa tacgtgccac attatcattg ttacgctggt aaagcgacat
     2041 cgtgtctgcc atataaaatg tgggattcaa cttataatgc tcccatggca tacgggaaaa
     2101 ttccttcaac agagaaagcg cgtcatattg gtaagccaca tcctgacgga tggtatcgtt
     2161 cctatttcta ttggccgcca tgtaaacata gtcaatgtgc cagtgatcga gacttcctga
     2221 caaattacca atgttgcgaa aacggaaccg gaacccattt tcaaagaaca tattctgcag
     2281 tacaggaacc aatacctgcc tgaaatcatt aaatgccaga gcaccgggac ttgcccattg
     2341 acgaaaccaa tcgtttgcac ttggcgacca aaactccagg atgagtgaat cacccacttc
     2401 cggttgattg cctcttcctg tttgctgata gaaaaaactc aggtaaacag aatcggcagc
     2461 tgtgtaagga aaagtattgg gtttaatatc caaatcgata ggcttgcttg taaggtaatc
     2521 gcaaaccccg gttaatgtag tattgacaaa atcgtatgga tatccctcat cattcaaacc
     2581 gtcgagcgtt gcaacaccaa tggttggagg accaattgga aaacgattat tcaggtaggc
     2641 gaagcgatcg acccaaagtg aattatctcc tgcagaagac acaaaattta cataaaccga
     2701 atcttgaacc aaatctacat tctgaatata aattgtatct ggagaagagg gctgccaaag
     2761 cgtatcgata atggaataac gtggataaac atactcctgc acaccagtta tggggtaaga
     2821 agtaaggtca ttaacggtaa tgagcacgct atccaatgga tttattgttg taaccaaaac
     2881 tcctgcaata ctgtcgtagg taatgtaata tgtaggattt cgcatatact ttacggaatc
     2941 atgcaggaga acggttccgg cagcatcata aaggcgaaaa aaggtttgtg atgtaacatt
     3001 tgcatcaccg gcctgtgcat tgtagggctt aaagcgatca cgactgaaat catccagaaa
     3061 aggaaggaga atagtatcga tttcgtaaac aaaaagcgaa ggaaacatcc tcacttcttt
     3121 tttggtactg gtcagatcaa aattctcgga tagcggactg agtgcttcgt tttgtgcgta
     3181 tgaatatgtg cacaactgca ccaatgtgca gatcagaata agtgcttttg ttttttggcc
     3241 ctgcatttga aagaatggct taatcgttac gtaaccaaac agtaacctct gaacccgatg
     3301 aagcctctcc accagcaccc ggagattgac ggtagatccg ggcactgaca gagtctttgc
     3361 gggttacgca tccttcacag gaagcagcat agagactgag tgctactctg tttaaactcg
     3421 aattagcttc atctattgtc ttaccggtta aatcaggtac cgtttctgta cctccacccc
     3481 tgccctgacc cagaaccagt tcgatggtac tacccttttc cactttatcc cctttggaaa
     3541 ttggacgtgc cttatatttc tgttcaatca cacaatcctt acaatccgga taaggcttat
     3601 aagtgtactt aaccttgaga ccaacaatct ttaatattcc ttctgcatgc ttttttgact
     3661 tgtagaccaa atccggcata ctcaccatca gcggagattt tgaaacaact gtcaggtaaa
     3721 cggtccttcc ttctttcaca aattgccccg tagaatcagt tggcccagga tcctgacgaa
     3781 taacggtacc tctgggatat ttatcggtgc taacggaatc gatctggtaa cgaagtccga
     3841 gcgaagttaa tttcccatct atctcttcca gcggcatccc aataaaatca ggtagggtaa
     3901 tataattttc cttgccatga tgggtatagc ttttcaggta ccaaacggta caatagaaca
     3961 ataggatcat ggttaagagt cctgctccaa aatgaaacag gaactgcctg gagatgagga
     4021 aacgaccgat gttgccgagt gtgcgcataa gtgattaatg gacaaatata accccaattc
     4081 cgccacaatc ggaagagaat gagtaagcga ggcaaaataa ctaacaaacg catggggaaa
     4141 caatcggctt ttggagagta tccacgatgg gaagcaggtt acttttgtaa aaaatactcc
     4201 atgaaaaccc gcattgcagt cctgtcaggt ggatactccc acgaagctca gatctcaaga
     4261 aagagtgcct caaccgttat gcggcacctc ccaacggatc ggtatgattt aatgcaaatc
     4321 gatatttcgg aggaaggttg gtttgcgcta tccggtgatg aaaaaattcc ggtcgacaaa
     4381 aacgactttt cagtaatcgt aaatggcaaa aaacatcagt ttgatgctgc attcattgtt
     4441 atccacggaa ccccgggaga agatggaaaa ctgcaagggt atttcgatat gcttaagcta
     4501 ccgtacacta cttcgggtgt attgacctct gcattgagtt ttaataaatt cgcctgtaac
     4561 acactgcttt cccaaatggg aatacgctgt tcaaaatcgc tgattatccg aaagggagac
     4621 cccatcagca gtgaaaatat agctatgact attggctttc caatgttcat caaaccaagc
     4681 gatggcggtt ccagctttgg ggtttccaag gttaaacaag cagaggaaat agaaaaagca
     4741 atttctaccg cccttgagca tggtacagaa gcactgattg aagcctttgt taatggaaca
     4801 gaagtcacct gtggggtatt ttccgtaaat ggtaaagtca ctgttttaca tcctacagaa
     4861 attgtaacag aaaatgagtt tttcgatttt gatgcaaaat accaggggaa atcgaaagaa
     4921 atcacaccgg caagactgag tgaagaggta acaaacctgg tgcgagccac cacagcagag
     4981 gtatatcgat tgcttaatct tcgtggtatg gcacgtgtcg attttatcat tcaggaaaat
     5041 gttccgttca tgattgaagt gaatactgtg cccggacttt cagatgccag tattttgcct
     5101 caacaggccc gacatgcagg catagaatta agcaccttat tttcttcagt tcttgaggat
     5161 gcgcttaagc gttgagttca tttagaaaag cgatgagttt ttcaactgct tttgcccgat
     5221 gactgcattt attcttctcc tgagcgctca tctcggcaaa ggttaaggaa cttccttcgg
     5281 ggacaaacaa ggaatcgtat ccaaaaccac cactgccacg acgttcggtg atgatgtttc
     5341 cgttcataac tccctcgaac aagtattctt ttccatcgag aagtaatgca attactgttt
     5401 taaaacacgc acttcggtca acttttcctt gcagttcgag cagtaacttt ttcatattcg
     5461 cttctgcact cttctgttcg ccggcatacc gtgcagaata gactccggga gccccattca
     5521 gggcagaaac ctcaagtccg gtatcatctg caaaacaatt caccccaaat cgacctgaaa
     5581 caaaacgagc tttttgcaat gcgttttccg taagtgtact gccggtttca ggaatttctt
     5641 ccttcaaatt gtattcatcc agacccgaaa tctgaatgga atcgtccaat aaagccgcaa
     5701 tctcatctat cttatgttgg ttctgggaag caaaaacgat cttcatatca aatttttccc
     5761 caaagttaat gctttataaa tgctataagg cagtaagtga gtatctttgt acgaactact
     5821 tgtgcgggga tgttggataa cataacacgg aaaatagatt taatgatcat tggcgcccag
     5881 aaatccggga ctacctcgct gctgcgttat ctgggagaac atccgcaaat agaaagccat
     5941 aaagcaaatg aattcagtta tttctattcg gatgaagaat atgaaagtgg ttgggcaaag
     6001 gctgaggaat attatttcgg gaaaaaaaat tcagataaaa actttgttgt tgccaagcat
     6061 gcgaatttgt attcatcggc taaagcaata gagcggcttt atgaacagtt tcctcaatgc
     6121 aagttggtat tgttattgag aaatcctgtt tccagatcct actccagcta cctgatggaa
     6181 aaaatgtacg atcgtgtcga ctatgaattt gatgaattgc cggaattgtt ggagaaaaaa
     6241 gatggaaggc aactcgaatc ctggcaagaa gaggcattta ttggt
//
LOCUS       DAOMYG010000151         6263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4239_length_6263_cov_8.731637, whole genome shotgun sequence.
ACCESSION   DAOMYG010000151 DAOMYG010000000
VERSION     DAOMYG010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4239_length_6263_cov_8.731637"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PK610_06235"
     CDS             <1..378
                     /locus_tag="PK610_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRJ35565.1"
                     /translation="DFIQRYGKKDRTERNVLMISRLTTVGWGIIAIGVAMFANRMGSL
                     IEAVNVLGSLFYGTMLGIFLVAFMFKFIKGNAVFYAAILAEVVVVILFIRDDAAFLWL
                     NMIGCIAVILFSILIQTLIPKKS"
     gene            550..1218
                     /locus_tag="PK610_06240"
     CDS             550..1218
                     /locus_tag="PK610_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRJ35566.1"
                     /translation="MQNRINNNYWQPQPYIENLHFEESQIYHVYNRANGSELLFRNDN
                     NYYFFLRKIKQYISPVCTIYALCLMPNHFHLLIRIKEKKEVLDFIKSEENKIKSDEEN
                     FCMHEITSMQFRRLQISYAKALNKQLSRKGSLFIQNIKRRNIDSDQYFFNVLDYIHNN
                     PVNSGFSYRAGDWKFSSFNSMVNDKGCCSGWIARDELIAICGGQSELKIILTNKIGSL
                     SAAY"
     gene            complement(1240..2115)
                     /locus_tag="PK610_06245"
     CDS             complement(1240..2115)
                     /locus_tag="PK610_06245"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HRJ35567.1"
                     /translation="MIKKSIFMMGLSLAITMLASENLFGQLSVPAPSPTITVKQGFAT
                     SNIELSYSRPSVRGRVVFGDLVPYDKVWRTGANGATTIYFGEEVSVGGKKLPKGKYGL
                     LTIPGKSEWTVILSKDTTVTSPADYKQENDAVRMKIKPVKLPAMVETFSIEIANVRTK
                     GDGCDIRIVWERTMISFTVTADIDTKIMAQIDESMKGDKKPYYQAARYYYENKKDLRK
                     ALEWADEAVKARPDAYWIILLKARIQFDLGDYDGCIATANQSKEKAKADQNDDYVKMN
                     DKLIADAQKLQLKKK"
     gene            2267..2845
                     /locus_tag="PK610_06250"
     CDS             2267..2845
                     /locus_tag="PK610_06250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010516310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRJ35568.1"
                     /translation="MANNFFAHTTAVIDEGAQIGEGTKIWHFSHIMPNCKIGNHCNIG
                     QNVVISPEVILGNNVKVQNNVSIYTGVTCDDDVFLGPSMVFTNVINPRSAVNRRDQYA
                     KTHVGRGVSIGANATIVCGNNIGEFAFIGAGAVVTKEVPAFALVVGNPAKQTGWMSEY
                     GHKLSFNTEGIAECPESGQRYELKKGIVSRIS"
     gene            2855..3880
                     /locus_tag="PK610_06255"
     CDS             2855..3880
                     /locus_tag="PK610_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ35569.1"
                     /translation="MESKIKFGVIGQGHIGKRHAEMIRRNPECELVAVCDILPSEITN
                     SPAADLPYYSDVKPMLQSHPEIEVVSICTPNGLHAQHSLLALENKKHIVVEKPMALNK
                     RDCEEIIFKALQAHKHVFCVMQNRYSPPSQWIKSLIESEKLGKIHMVQLNCYWNRDDR
                     YYKKGNWKGSLDLDGGTLFTQFSHFIDIMYWLFGDIQNIQARFADYTHKDLTAFEDSG
                     IVNFEFSQGGMGCINYSTSVWDKNLESSITIIGENGSVKIGGQYMDQVEVCHIQDYKM
                     PELAPTNPANDYGHYKGSANNHHYIFTNVIDTLKGRTTMTTNAMEGLKVVEIIERIYA
                     LKNSNKG"
     gene            3901..5226
                     /locus_tag="PK610_06260"
     CDS             3901..5226
                     /locus_tag="PK610_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRJ35570.1"
                     /translation="MKKQLPSSAKNKDLYKLIAEKKESVAVIGLGYVGLPIALAFARK
                     IKVIGFDINAQRVEMMKKGIDPSNELAKEEFKGCDISFTANPADLKKAKFFVVAVPTP
                     IDEHNLPDLSPLLGASTTVGRALKKGDYVVFESTVYPGCTEEDCIPVLEKESGLKFKK
                     DFKVGYSPERINPGDREHTITKIKKVVSGCSAESLDIIAKVYELIIEPGVHRAATIKV
                     AEAAKIIENTQRDVNIALMNELSIIFNKMGINTFDVLEAAGTKWNFLRFYPGLVGGHC
                     IGVDPYYLTHKAEALGYHSRVINSGRYVNDSMGFYVAKNTVKKIIAAGKNISKSKVLV
                     MGATFKENVSDIRNSKVADVVKELKSFGVKVDVVDPHANSAELKHEYGFGLNKLGKGY
                     DGVIICVNHKEYLGLDEKFFTGILSKGGVLTDVKGIYKNKIKKITYWSL"
     gene            5226..6215
                     /gene="rfbB"
                     /locus_tag="PK610_06265"
     CDS             5226..6215
                     /gene="rfbB"
                     /locus_tag="PK610_06265"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRJ35571.1"
                     /translation="MGSTTKILITGGAGFIGSHVVRRFIKNYPSYQIYNLDKLTYAGN
                     LENLRDIDKSPNYHFIKGDIVDSEFIQQLFSEHDFDGIIHLAAESHVDRSIANPIEFV
                     MTNVVGTVNLLNAARKGDKKRRFYHISTDEVYGSLGTSGLFSEQTKYDPHSPYSASKA
                     SSDHFVRAYYDTYGLDIVISNCSNNYGPNHFPEKLIPLSINNIRNNNALPVYGKGENV
                     RDWLYVEDHASAIDLIYHKGKSGETYNVGGLNEWKNIDLIRFLCKTMDVKLGREIGTS
                     ERLITFVKDRAGHDLRYAIDASKLRNELGWKPSVTFEEGLEKTVEWYLRNTAR"
BASE COUNT         1975 a         1213 c         1273 g         1802 t
ORIGIN      
        1 gatttcattc aacgttatgg caaaaaagac agaacagaaa ggaacgtgtt aatgatatca
       61 cgtctcacaa cagttggatg gggaattatt gcaattggag tcgcaatgtt cgcaaataga
      121 atgggcagcc ttattgaagc agttaatgta ttaggatcac ttttttacgg aactatgctg
      181 ggaattttcc tggttgcatt tatgtttaag tttattaagg gaaacgctgt tttttatgca
      241 gcaattcttg cagaagtagt ggtagtaatt ctatttataa gagatgacgc tgcctttctg
      301 tggcttaata tgattggatg tattgctgtg atactatttt ccattcttat tcaaacactc
      361 atccccaaaa aatcctgatc gaactgttca cttaacagtg attttggtat ttattcgcta
      421 ggttttttct tctgcgcaaa agaactttgc ccaactagtc tattccagta gacctccggg
      481 atttttaaaa tcccggaggt ctgcggggaa taatggacag gggatacaac tttcaattta
      541 ttctcaacca tgcaaaaccg aataaataac aattattggc agccacaacc ttatatagaa
      601 aatctgcatt ttgaggagtc acagatctac catgtatata atcgggcaaa tggaagtgaa
      661 ttactatttc gtaatgacaa caattattat tttttcctta ggaaaataaa acaatacatc
      721 agtcctgttt gcactatata tgcattatgt ttgatgccta atcactttca tttacttatt
      781 cgcatcaagg aaaaaaaaga agtgcttgat tttattaaat cagaggagaa taaaatcaaa
      841 tctgatgagg agaatttttg catgcatgag attacctcca tgcaattcag gagactgcag
      901 atttcttatg caaaagcact taacaagcaa ttgagcagaa aagggagctt gtttattcag
      961 aatattaaac gaagaaacat tgactctgac caatactttt tcaatgttct ggattacatc
     1021 cacaataatc cggtcaatag tggcttctcg tatcgtgctg gtgattggaa attttcatcc
     1081 tttaacagca tggtgaacga caagggatgc tgttcgggat ggattgccag agatgagtta
     1141 attgcaatat gcggcggaca atcagaattg aaaattattt taacaaacaa aataggcagc
     1201 ctttcggctg cctattgaat attccttaaa cggaaaaaat tattttttct tcaattgtaa
     1261 tttttgtgcg tcagcaataa gcttgtcgtt cattttcacg taatcgtcgt tctgatcagc
     1321 tttagctttt tcctttgact gattagcagt agcaatgcaa ccatcgtaat caccaagatc
     1381 gaattggata cgtgccttta gaagtatgat ccaatatgca tccggacgag cctttacagc
     1441 ttcatctgcc cactcaagtg ccttacgcaa atcttttttg ttctcgtaat agtaacgtgc
     1501 agcctggtaa tatggctttt tatcgccttt catggattca tcaatctgag ccatgatttt
     1561 agtatcgata tcagcagtaa cagtgaaaga aatcattgta cgctcccaaa caatgcgaat
     1621 atcgcaaccg tcgcctttgg tgcggacatt ggcaatttca atactgaagg tttctaccat
     1681 tgcaggcagt ttaaccggct tgatcttcat acgaaccgcg tcgttttcct gcttgtaatc
     1741 tgcaggtgat gttacggttg tatccttact caggataact gtccactctg atttacccgg
     1801 aattgtaagc aaaccgtatt taccttttgg aagtttttta cctccaacag aaacttcctc
     1861 tccaaaataa attgtagttg cgccattggc accggttctc catactttgt cataaggaac
     1921 caggtcacca aaaacaaccc ttccacgaac tgaaggacgg ctgtaactta attcaatgtt
     1981 cgatgttgca aatccttgct taactgtaat tgtaggactt ggggcgggaa cgcttagctg
     2041 tccaaaaagg ttctcggaag ccaacattgt gattgccagc gataaaccca tcataaaaat
     2101 gctcttcttg atcataatgt ttttattttt ttcaaatgta agcgattaat tgtcattaca
     2161 atgattttcc gtcacttaaa cagtggttta tgaattttgt tcaattcatc gtattatcaa
     2221 tccttattgc ctcaggttga gtatatttgc cattctcaaa atcgccatgg cgaacaattt
     2281 ttttgcacat acaacggctg tgatagatga aggggctcag attggtgaag gaaccaaaat
     2341 ctggcacttc agtcacatca tgcccaattg caaaatcgga aaccactgca acattggcca
     2401 gaatgttgtg atctcacctg aagttattct gggaaacaac gttaaagtgc aaaacaatgt
     2461 gagcatttat actggtgtta cctgtgatga tgatgttttt cttggacctt cgatggtttt
     2521 taccaatgtg ataaatcccc gcagtgcagt taaccgtcgc gatcaatatg caaaaacaca
     2581 tgttggaaga ggagtttcaa taggtgccaa cgcaacaata gtttgcggaa acaacatagg
     2641 agaatttgca tttatcggcg ctggtgcagt tgtaaccaaa gaagttcctg catttgctct
     2701 tgttgttggc aatcccgcaa aacaaaccgg gtggatgagc gagtatggac ataaactttc
     2761 ctttaacacg gaaggaattg cagaatgtcc ggaatcgggt cagcgctacg aattaaaaaa
     2821 aggaatcgtt tcacgtatca gctaattttc tatagtggag agtaaaatta aatttggtgt
     2881 aataggtcag ggccacattg gcaagcgtca cgcagaaatg attcgccgta acccggaatg
     2941 cgaactggtg gcggtttgcg atattcttcc ttcagaaatc acaaactctc ctgcagcgga
     3001 tctgccttat tattccgatg taaagcccat gcttcagtct caccccgaaa ttgaagtcgt
     3061 tagtatttgt acccccaacg gattacacgc ccaacattcg ctgcttgcac ttgagaacaa
     3121 aaaacatatt gtggttgaaa aaccaatggc attgaataag cgcgattgcg aagaaattat
     3181 cttcaaagca ttgcaagccc ataaacatgt gttttgtgtc atgcaaaaca gatactctcc
     3241 cccttctcaa tggatcaagt cgctgattga atccgaaaaa cttggaaaaa ttcatatggt
     3301 ccaactgaat tgctattgga acagagatga tcgttactac aaaaaaggga attggaaagg
     3361 aagtcttgac cttgacggcg gcacattgtt tacccaattc tcgcacttca tcgatatcat
     3421 gtactggctg tttggagata ttcaaaatat tcaggctcgc tttgctgact atacccataa
     3481 agatctcact gcatttgaag attcaggaat tgtaaacttt gaattcagtc aaggcggaat
     3541 gggatgcatt aactactcca cttctgtatg ggataaaaat ttggaaagct caataactat
     3601 cattggtgaa aacggaagtg taaaaatcgg cggtcaatac atggatcagg ttgaagtttg
     3661 tcacatccag gattacaaga tgccagaact tgctcctacc aatcctgcca acgactacgg
     3721 tcattacaaa ggatcggcaa ataatcacca ttacattttc accaatgtaa tcgatacact
     3781 taaaggcaga actaccatga ctaccaatgc gatggaagga ctaaaagtag ttgagattat
     3841 agaacggatt tacgcactga aaaattccaa caaaggataa ccaaaggata ttatttgaat
     3901 atgaaaaagc agttaccatc ttctgccaaa aacaaggacc tctacaagct gattgctgaa
     3961 aaaaaagaat cggttgctgt tataggatta ggttatgtag gcttacccat tgcacttgca
     4021 tttgcgagaa aaattaaagt gattggcttc gacatcaatg cccagcgcgt tgaaatgatg
     4081 aaaaagggaa ttgatccaag taacgaatta gcaaaagaag aatttaaggg ttgcgatatt
     4141 tcattcactg caaatcccgc tgatctgaaa aaagctaaat tttttgtggt tgcagtaccc
     4201 actcctatag atgaacataa tcttcctgac ctgagtccgc tgcttggtgc atccactact
     4261 gttggaagag cacttaaaaa gggagattat gtcgtatttg aatcaacggt atatcctggt
     4321 tgcacggaag aagattgtat accagtactt gaaaaggaat cgggcttgaa attcaagaag
     4381 gattttaaag taggttactc tcctgaacgt atcaatccag gagacagaga acacacaatt
     4441 acaaaaatta aaaaagtggt ttcaggatgc agtgcagaat cgcttgacat cattgctaaa
     4501 gtatatgagc tgatcattga gcctggcgtg catcgggctg ctaccattaa agttgcagaa
     4561 gctgcaaaaa tcattgaaaa cacccagcgc gatgtgaata ttgcattgat gaatgaattg
     4621 tcaatcatct tcaataaaat gggcataaac acctttgatg tactcgaagc tgccggaaca
     4681 aaatggaact ttcttcgctt ttatccgggt ttggttggcg gacattgtat tggtgtcgac
     4741 ccgtattatc tcacccataa agctgaagcc ctgggttatc attcgcgggt aataaacagc
     4801 ggaagatatg taaatgattc aatgggattt tacgtagcga aaaacacagt aaaaaagatc
     4861 atcgcagccg gaaagaacat aagcaaatcg aaagttcttg ttatgggggc cacattcaag
     4921 gaaaatgtaa gcgacatccg aaattcgaaa gttgcagatg tggttaaaga attaaaatcc
     4981 ttcggtgtaa aagttgatgt tgtagatccg catgccaact ctgccgaact taaacatgag
     5041 tatggatttg gattaaataa attaggcaag ggatacgatg gtgtaattat ctgcgttaat
     5101 cataaagagt atttaggtct cgacgaaaaa ttcttcaccg gaattttaag caaaggagga
     5161 gtactcaccg atgttaaggg aatctataag aacaagatta aaaaaatcac ttattggagt
     5221 ttataatggg cagcacaaca aaaatactca tcaccggagg tgccggtttt atcgggtcac
     5281 acgttgtaag aagattcata aagaattatc cttcttatca gatttacaac ctcgataagc
     5341 tcacctatgc cggtaacctg gagaacttgc gcgatattga taaatcacca aactatcatt
     5401 ttattaaagg tgatatcgtg gacagtgaat tcattcaaca attattcagt gaacacgatt
     5461 tcgatggcat tattcacctc gcagctgaat cgcatgtcga ccgttccatt gctaatccaa
     5521 ttgaatttgt aatgaccaat gtagttggca cagtaaatct gttaaatgct gcacgtaaag
     5581 gagataagaa aagaagattc tatcacatca gtaccgatga agtttatggt tcacttggca
     5641 caagcggatt attttcagaa caaaccaaat acgatccgca ttcaccatac tctgcatcca
     5701 aggcatcatc cgatcatttt gtccgcgctt attacgacac gtatggtttg gatatcgtaa
     5761 tttccaattg cagcaacaat tatggtccca accatttccc tgaaaaactg attccacttt
     5821 ccattaataa tatccgcaac aacaatgcgc ttccggttta cggaaaagga gaaaatgttc
     5881 gcgattggtt gtatgtagaa gatcacgctt cggccattga tttaatttac cataaaggaa
     5941 aatccggcga aacatacaat gtgggaggat tgaacgaatg gaaaaacatt gatctgattc
     6001 gtttcctgtg caaaaccatg gatgtaaaac ttggtcgtga aattggcact tcagaacgac
     6061 tcatcacctt cgtaaaagac agagccggcc atgatctacg ttatgccatc gatgcttcca
     6121 aattgcgcaa cgaactggga tggaaacctt cagttacgtt tgaagaagga ttggaaaaaa
     6181 cagtagaatg gtacctccga aacactgcac gttgatgttg ctgccgccgc caacgccgaa
     6241 tccctggagg aagtgccggg acg
//
LOCUS       DAOMYG010000152         6257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4245_length_6257_cov_8.115285, whole genome shotgun sequence.
ACCESSION   DAOMYG010000152 DAOMYG010000000
VERSION     DAOMYG010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4245_length_6257_cov_8.115285"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PK610_06270"
     CDS             complement(<1..375)
                     /locus_tag="PK610_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35572.1"
                     /translation="MMKKKITGFLMVLLAMQSCQTSAPQPENASSEDSVSTHVDSVPE
                     LNSTEAYSQFEQLYLKGNSSEVPETVCTELFGKSSDFPGSPMFTVRTIGMADAKKTFK
                     GFLLTNNLSNYLAVFSQDEKKEL"
     gene            complement(372..956)
                     /locus_tag="PK610_06275"
     CDS             complement(372..956)
                     /locus_tag="PK610_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HRJ35573.1"
                     /translation="MKYNLSEITAVIQDRRTIAPEFFSDRKVPREIVEKILNNAIWAP
                     NHGMTQPWRFKVFMGDSIAKFGEAHAELYKASVNETDFDPGKYSKLKDRPLLSSVVIA
                     ICMKRNEAGKIPEIEEVEAVACAVQNMYLTCTAYGLASYWGSGGMTYSEGMKEYLGLG
                     AQDKCLGFFYIGYPKGEWPKGQRRPIEYVTEWME"
     gene            1110..3338
                     /locus_tag="PK610_06280"
     CDS             1110..3338
                     /locus_tag="PK610_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ35574.1"
                     /translation="MKACLVSICLSVALTVNAQVNEPDTTTVLSEVTILGKSANTLPG
                     SGAVIEARKLSQFNQPDINKVLRTVPGVNVRDEEGFGLRPNIGLRGTQVNRSAKITLM
                     EDGVLMAPAPYADPSAYYFPTFARMESVEVLKGSSQIKYGPYTIGGALNLISTSIPNA
                     FRGFAQFSYGSFGTNQQRIWIGDSHKNFDYVFEVNRIASNGFKELDNKGNTGFDRRDV
                     MGKMRWHTDASAKLQQSLTLKFVNMTEQGNESYLGLTFDDFKANPNRRYSATQKDILD
                     MKHHHVSLTHSITPLKGFSIVTTGYYASTFRDWARVNTIAGKSVNAILADPVANDTAY
                     QVMTGNTNGTVAYQSAARTYTVKGIQTNLKFELQTGSVKHIFQAGVRYHEDQADRYAT
                     RSTYNMLNGTMILTTPGVTGNNENQIRGAKSVASYFQYDFNFKKLTVSPGIRHEQITF
                     DIMNYGTSDNARMGTALKTASNQLSILLPGIGINYAIKDYMSVFGGAHKGFSPPGMPS
                     TTTTVQAKVETAMNYELGYRYNKNGFKTQVVGFYNDYDNILGSDNVSGGGAGTGDMYN
                     AGNATIMGAEVSIEYNILSRLNKENNIRMPLGIAYTFTDAKFDETFINAGGDWGTGTI
                     NQGDYIPFITPHLFTATLGLETNKFNTTLISRYVGDTRTIPGQGELITPGENVKYNDV
                     NTIAGYWVFDLSANYRFYQGVSAYLSANNLLNTKYIVANLPQGYRPGMPFAVNVGLKA
                     NF"
     gene            complement(3433..4704)
                     /locus_tag="PK610_06285"
     CDS             complement(3433..4704)
                     /locus_tag="PK610_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRJ35575.1"
                     /translation="MEPLAERMRPGKLENYIGQEHLIGENAILRKTLATGNIPSMILW
                     GPPGVGKTTLAQLISTQLKRPFYTLSAINSGVKDIREVIEKVGNQGLFGGNSAVLFID
                     EIHRFSKSQQDSLLGAVERGIITLIGATTENPSFEVIPALLSRCQVYVLRHLEKEDLE
                     KIIRQAIANDELLNKREIELIETDALIKISGGDARKLLNALELVIQSSSPGKISITNE
                     SVTSVIQNNLALYDKNGEQHYDIISAFIKSIRGSDPNAAVYYLARMVEGGEDPLFIAR
                     RMLILASEDIGNANPNALLIATSTFQAVSMVGWPESRIILSQCALFLATSPKSNASYE
                     AINAAQELVRTTGSLSVPLSIRNAPTRLMKELGYGEKYKYAHSYKGNFAEQEFLPEAI
                     SGTKIYNPGNNKREEEIRAWLKARWKEKYGY"
     gene            complement(4706..5446)
                     /locus_tag="PK610_06290"
     CDS             complement(4706..5446)
                     /locus_tag="PK610_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018942041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRJ35576.1"
                     /translation="MKAVIIDDNSESRASLRADIENFCPEIEIIAEADSVVSGTKLLR
                     QVQPQIVFLDIQMGDGSGFDLLEILGDVSFNVIFTTASDAHAVKAFRFSAIDYLLKPV
                     DPDDLIKAVAKIQGSKKTSGESLDLLMEHSKNGNKPLKRLALNTLDKIHVVNISDIVR
                     CEADVNYTMFYFLDKSKVLVTKTLKDFEEMLSDHSFLRVHQSHLVNTNYIKEFVKGDG
                     GYLLMQEGSNVPVSTRKRNAVVEALNTL"
     gene            5523..>6257
                     /locus_tag="PK610_06295"
     CDS             5523..>6257
                     /locus_tag="PK610_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35577.1"
                     /translation="MRLQTLVFAIISFLFTSGTVHSGGSAWPGMPYHHVKVYLYNLDG
                     KLFGRHSPIKEGIPDPTLFGEGVTLNTESQNALNKWLRQDLSLLNEGMAGCYIPHHAI
                     IFYDDQNKPIAWMSACFMCTGIRFYFPVPKMKKVVYNSKTEQMANRQIADLRKIVEGA
                     GIPVFDTAEEYGKYMQKNKKVSNERVCLDTAFIRNYYPEKLTMDVLMKSISADSIDAK
                     FSTTHHAKVTAGGDAYSFYECRYKTSR"
BASE COUNT         1813 a         1294 c         1289 g         1861 t
ORIGIN      
        1 caactctttt ttttcatcct gcgaaaaaac agcaaggtag ttggagagat tattggtaag
       61 gagaaatcct ttgaacgttt tttttgcatc tgccattcca atcgttctta ccgtaaacat
      121 tgggctgccc ggaaaatcgc tgcttttccc gaatagttcc gtgcatacag tttccggaac
      181 ttcagatgaa tttcctttaa ggtataattg ctcaaactgt gaatatgctt ctgtagagtt
      241 aagctccgga acggaatcaa catgagtgga aacagaatcc tctgatgagg cgttttcggg
      301 ttggggtgcg ctggtttgac aggattgcat ggcaagaagc accatcaaaa aaccggtaat
      361 tttttttttc atcattccat ccattcggtt acgtactcaa taggacgtcg ttgtcccttg
      421 ggccattctc cttttggata accaatatag aaaaatccga ggcatttatc ctgagcgccc
      481 aatccaagat actctttcat cccctccgaa taggtcattc cccctgatcc ccaatacgaa
      541 gccaatccat atgcagtaca ggtcaagtac atgttctgta cagcacaagc taccgcctcc
      601 acttcttcaa tctccggaat ttttcccgcc tcgttgcgct tcatgcaaat agcaatcact
      661 actgaagaaa gtagcggtcg atcttttagc ttggaatatt ttccgggatc aaaatcagtt
      721 tcgttcaccg atgctttgta taattctgcg tgtgcctcac caaattttgc gatcgaatca
      781 cccataaata ctttaaacct ccagggttgc gtcatcccat gattgggagc ccagatggca
      841 ttgtttaata ttttttctac gatttcacgc ggaacttttc ggtcactgaa aaactccggt
      901 gcgatggttc ggcggtcctg aattactgct gttatttcac ttaagttgta cttcatctgt
      961 tccggctttt ttacaaaatt aggacttgaa ctgcatcagc cgattttatt tcccttttat
     1021 tcaaaatacc tgctttgtgg tattgtctga acatgctgcc cattctactt tcgtgtcgta
     1081 atttataatt aatccaaaca aaaataagga tgaaggcatg tcttgtatcg atctgtttat
     1141 cagtagcttt aaccgtaaat gcgcaagtta atgaaccgga tactacaacg gttttgtcgg
     1201 aagtaaccat ccttggtaaa agcgccaata ccctacccgg atccggagca gtaattgaag
     1261 ccagaaagct ttcccaattc aaccaaccgg atataaataa ggtacttagg acagtgcccg
     1321 gagtaaatgt aagagatgaa gaaggctttg gcttgcgccc aaacatcggg ttaagaggaa
     1381 cacaggttaa ccgaagtgca aaaattactt taatggaaga tggagtgttg atggctcctg
     1441 ctccctatgc ggatccttct gcctattact tcccaacttt tgcgcgtatg gaaagcgtag
     1501 aggtgttgaa aggaagcagt cagatcaaat acggtcctta tacaatcgga ggcgcattaa
     1561 acttaatatc cacatcaatt cccaacgcat tcagaggctt cgcacagttt tcttatggga
     1621 gttttggcac aaatcagcaa agaatctgga taggtgacag tcataagaac ttcgattatg
     1681 tttttgaagt aaaccgtatt gccagcaacg gatttaagga attggacaac aagggtaata
     1741 cggggtttga tcgcagagat gtgatgggta aaatgagatg gcatactgac gcatccgcta
     1801 agttgcagca atcacttacc ttgaagtttg taaacatgac tgagcaagga aatgaatctt
     1861 atctcggttt aacatttgat gattttaaag ccaatccaaa cagaaggtat tccgctacgc
     1921 aaaaagatat tctggatatg aaacatcacc atgtttcttt aactcattcc attacacctt
     1981 taaaaggatt ttcaattgta actacaggtt attatgcgag tacattcaga gattgggcac
     2041 gtgtaaatac aattgcagga aaaagcgtca atgcgattct tgctgaccct gttgcaaatg
     2101 atactgcata ccaggttatg accgggaata caaatggtac agttgcttat caaagtgctg
     2161 cacgaaccta taccgttaag gggattcaaa ccaatctgaa gtttgagctt cagacaggtt
     2221 cggtcaagca tatatttcag gccggagttc gttatcatga ggatcaggcg gaccgctatg
     2281 caacacgttc tacctacaat atgctcaacg ggacaatgat tctgactaca ccgggtgtta
     2341 caggaaataa tgaaaatcag atacgtggcg caaaaagtgt agccagttat tttcagtacg
     2401 attttaattt caaaaaacta acagtaagtc ccggaatccg ccatgagcag atcacatttg
     2461 atatcatgaa ttatggtact tcagacaatg cccgcatggg aacagccttg aaaactgcat
     2521 ccaatcaact ttcaatttta cttcccggaa taggaattaa ttatgccatc aaagattata
     2581 tgagtgtttt tggtggagct cataaaggat tctctcctcc tggaatgccg tctacaacca
     2641 caacagtaca ggcaaaagtt gaaaccgcga tgaattacga attgggttat cgttacaata
     2701 agaatggatt taaaacacag gttgtagggt tttataacga ttatgataac atccttggtt
     2761 cagacaatgt ctcgggtgga ggagcaggaa caggtgatat gtacaatgcc ggaaatgcaa
     2821 cgattatggg agcagaagta agcattgaat acaatatcct ttcgcggtta aataaagaaa
     2881 acaatatacg tatgccattg ggaattgcct acacatttac agatgcaaaa tttgatgaaa
     2941 catttataaa cgcaggtggc gactggggta caggaacaat caatcaagga gattatattc
     3001 catttattac tccgcatttg tttactgcaa cattaggcct tgagacgaat aaattcaata
     3061 caacacttat ttcaaggtat gtgggcgata cccgcacaat cccgggtcag ggtgaattaa
     3121 tcacgccggg cgaaaacgta aagtacaatg atgtgaacac cattgcagga tattgggtgt
     3181 tcgatttaag cgcgaattac agattttatc agggtgtgtc agcgtatttg tcggccaata
     3241 atctattgaa cacaaaatat attgttgcaa accttccgca aggatacaga ccgggaatgc
     3301 catttgctgt aaatgttgga ttaaaggcaa acttttaaat tcttctgttc acacctgtaa
     3361 ttattcactt catcagtgcg taattgcagg tgagaatggc actttaaaat ccgtttgaag
     3421 aagggagctt gatcaatacc cgtatttttc tttccatcgt gcttttaacc aggcacgaat
     3481 ttcttcctcg cgtttgttgt tgccgggatt gtaaattttt gttccgctaa ttgcctccgg
     3541 taaaaattcc tgttccgcaa aattaccctt gtaggagtgc gcgtatttgt atttttcacc
     3601 gtatcccagt tctttcatca agcgcgttgg tgcattgcgt atggagagtg gtacagacag
     3661 acttccggtt gtgcggacta attcctgtgc tgcattaatg gcttcatagg atgcgttgct
     3721 ttttggcgag gtagcaagaa acagtgcgca ctgcgacaga attattctcg actccggcca
     3781 acctaccata ctcactgcct gaaatgtgga tgttgcaatc aacaaggcgt tgggatttgc
     3841 attgccgata tcttctgaag caagaataag cattcgtcgc gcgataaata gcggatcttc
     3901 tccgccttct accattctag ccaggtagta gacagctgca ttcggatcac ttcctctaat
     3961 cgatttaatg aatgcagaaa tgatatcgta atgttgttct ccgtttttat cgtaaagtgc
     4021 aagattgttt tggatgacgg atgttaccga ttcgttggta atactgatct tgccggggga
     4081 agaagattgg ataaccagct caagtgcgtt cagtaatttt ctggcatcac cacctgatat
     4141 ttttattagc gcatctgtct cgatcaattc aatttcccgc ttgttcagca gctcatcatt
     4201 tgcaatcgcc tgcctgatga ttttttcgag atcttctttt tcaagatgcc gtaaaacgta
     4261 aacctgacac cgggatagca atgcgggaat tacctcgaac gatggatttt cggttgtagc
     4321 accaatcaga gtaatgattc cgcgttcaac agcacccaat aatgaatctt gttgcgattt
     4381 actgaaacgg tgaatttcat caatgaacag aaccgcactg ttgccaccaa ataacccctg
     4441 gttgccaact ttctctatga cttcacgaat atccttaaca ccggaattga tggcgcttaa
     4501 tgtataaaag gggcgtttta attgagttga aataagctgg gcaagtgttg tctttcccac
     4561 accgggaggc ccccacaaaa tcatcgatgg aatattcccg gtagcaagtg tctttcgtaa
     4621 aatcgcattt tcaccaataa gatgctcctg cccgatataa ttttcgagtt taccgggtcg
     4681 cattcgttcg gctaagggtt ccatattaca aagtattcaa tgcttcaact acagcattgc
     4741 gtttacgtgt agaaaccgga acattcgatc cttcctgcat gaggaggtat ccaccatcgc
     4801 cttttacaaa ctctttgatg taatttgtgt taaccagatg gctttggtgt acgcgcaaaa
     4861 acgaatgatc acttaacatc tcttcgaaat ccttgagcgt ttttgttacc agtactttgc
     4921 ttttatcaag gaaataaaac atagtataat tcacatctgc ttcgcaacgt acgatatcag
     4981 aaatatttac cacatgtatt ttatcaagtg tatttagtgc taatcttttt agtggtttat
     5041 tcccgttttt ggaatgttcc attaaaagat ccaatgattc cccggaagtc tttttactac
     5101 cctggatctt tgcaactgct tttatcagat catccggatc aaccggcttg agcagataat
     5161 cgatcgctga aaaacgaaat gctttcacag catgtgcatc agatgccgtt gtgaaaatta
     5221 cgttgaatga aacatctcca agaatttcca gcaaatcaaa tccgctgcca tctcccattt
     5281 gaatgtcgag aaatacgatc tgtggttgaa cctgccgcaa taattttgtg ccgctaacca
     5341 ctgagtcagc ctcagctatg atctcaatct ccggacaaaa attttcaatg tcggcacgca
     5401 agctggcacg cgattcgctg ttatcatcaa taattactgc tttcatgtgg atcggaatta
     5461 aaacaaatat acaaaacgca ggccccaatt ggattcagtt ttttacgata ttgtaagtag
     5521 ttatgagact tcaaacactt gttttcgcaa ttatttcttt cctattcacg tcaggaactg
     5581 tacacagcgg aggttcagca tggccgggaa tgccttacca tcacgttaaa gtgtatttat
     5641 acaatcttga cggtaaatta tttgggcgac attctcctat taaagaggga attcctgatc
     5701 ctaccttatt tggtgagggt gtgacattga ataccgaatc tcaaaatgca ctgaacaaat
     5761 ggttgaggca ggaccttagt ttactgaatg aaggtatggc aggatgttat attcctcatc
     5821 atgcaattat tttttatgat gatcaaaaca aacctattgc atggatgtcg gcatgtttca
     5881 tgtgcacggg aattcgtttc tatttccctg tccccaaaat gaaaaaagta gtttacaatt
     5941 cgaagaccga gcaaatggcc aataggcaga tagcggattt acggaagatc gttgaaggtg
     6001 caggaattcc ggtttttgat acagcagagg aatacggaaa gtatatgcaa aaaaataaaa
     6061 aggtttctaa tgaaagggtt tgtttggata ctgcctttat tcgaaattat taccctgaaa
     6121 aattgactat ggatgtatta atgaagtcca tatcagctga ttcaatcgat gccaaatttt
     6181 ctaccacaca tcacgcaaaa gttacggcag gtggcgatgc ttattctttt tatgagtgcc
     6241 gttacaaaac ctcccgt
//
LOCUS       DAOMYG010000153         6213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4285_length_6213_cov_8.467197, whole genome shotgun sequence.
ACCESSION   DAOMYG010000153 DAOMYG010000000
VERSION     DAOMYG010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4285_length_6213_cov_8.467197"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..907)
                     /locus_tag="PK610_06300"
     CDS             complement(128..907)
                     /locus_tag="PK610_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35578.1"
                     /translation="MLFSLKAIAITAAIPFLTSDLPVGDAKTEGTTCNCTSLSTKENI
                     VVDGQTGQPFSGACLNEYRVEWISALKKKKMNNEMQAVVNGRKSINYYNNGILERTEE
                     QFSSGHKSAEYHFATGEKGAVVYHGAARQWHENGSNKYAGTFENGLPVGEHVWYDSRG
                     SRVELRKVTAVNTTNVTVDIEQTQWYDYQSKKSITRFTAIWDGKTAVTDKKNVSFFSF
                     YPSGKKKEVTIYDSMSSGYTKHEFYDEKGNLIQGGDKLQMK"
     gene            1108..2523
                     /gene="tig"
                     /locus_tag="PK610_06305"
     CDS             1108..2523
                     /gene="tig"
                     /locus_tag="PK610_06305"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRJ35579.1"
                     /translation="MNVTQEKIDDLNAVIKVKLTPEDYRPKYEATLKDYSRKAQVKGF
                     RPGHVPMAMIKKMYGKSILADEVNKLLNDSLYNYINENNLEVLGNPLPIEEQERVEFH
                     ENAEMEFRYEIGFAPKIDLNLSGFKVPYQALKVDDEMLNKQIDDLRRRYGKLVNAEES
                     IATDMLFGKFEELDENGNLKEGGISNTSTISIEFLKDEATKKQLTGKKPGDVVDLDPR
                     TVSQGESDLAAMLGVEREALADLHSKFRFTINEIKRIELAELNTELFDKLFGEGTIAT
                     EEDFRNRVSTDLKNMFAFDSDRLFLREATKQLLDNLSIALPDSFLKKWIVATNEKPIT
                     DEQLEKDYPSYARGLKWQLIENKIIKDNEIKVGNEEAVDYTKGYLVSQYNRYGLPAPE
                     EDQLNQSAMRILSNKEEAKRVYDELYSRKVLAFLKDAMNLAVTEHVYEEFMKIAGAQD
                     HNHDHEHDHDHEHDHEHHHHH"
     gene            2561..3229
                     /gene="clpP"
                     /locus_tag="PK610_06310"
     CDS             2561..3229
                     /gene="clpP"
                     /locus_tag="PK610_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRJ35580.1"
                     /translation="MFPKDEFQKYAVKHKGISSLHLEKYIEASMTPYIIEERQLNVAQ
                     MDVFSRLMMDRIIFLGTGIYDQVANIIQAQLLFLESVDAKKDVQIYMNSPGGSVYAGL
                     GIYDTMQFIAPDVATICTGMAASMGAVLLCAGAAGKRSALKHSRVMIHQPLGGAEGQA
                     SDIEITAREILKLKKELYEIISKHSGQPYEKVWQDSDRDYWMIADEAKSYGMIDEVLE
                     RHRK"
     gene            3274..4515
                     /gene="clpX"
                     /locus_tag="PK610_06315"
     CDS             3274..4515
                     /gene="clpX"
                     /locus_tag="PK610_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRJ35581.1"
                     /translation="MKEKNEVKCSFCGRSKRETNVLIAGITGHICDHCVSQAELIVKE
                     EVSSRTQGDLEHQLKLSKPAEIKTALDAYVIGQDDAKKVLSVAVYNHYKRLLQKGNTK
                     EDDVEIEKSNVILVGPTGTGKTLLAKTIAKMLNVPFCIADATVLTEAGYVGEDVESIL
                     SRLLQAADYDVAAAERGIVFVDEIDKISRKGDNASITRDVSGEGVQQALLKLLEGSVV
                     NVPPQGGRKHPEQKMIQVNTRNILFVCGGAFDGIDKIIARRVNTQTIGYSVEEEVHRI
                     DKENMLRFISPRDLKSFGLIPELIGRFPVLTYLDPLDADALRRILTEPKNALIKQYVK
                     LFEMDNIRLKFDKEVIDFMVEKAVEFKLGARGLRSICEAILNDAMFELPSDKNTETLH
                     ITLEYAREKFGKSKVMRLKVA"
     gene            complement(4516..5013)
                     /locus_tag="PK610_06320"
     CDS             complement(4516..5013)
                     /locus_tag="PK610_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HRJ35582.1"
                     /translation="MDFSFRNASINDLSEIVAIYNATIPGRMVTADLEPVTIESRKEW
                     FEKHNAQTRPLWVAEKNGKIAGWLSFHSFYGRPAYSITCELAIYIHSDFRGQKLGETF
                     LQKAIDSAPSLGVENLLGFIFAHNEPSLKLFRKFGFSEWGNLPDVARIDGKNVTLLIL
                     GKKTI"
     gene            5111..5905
                     /locus_tag="PK610_06325"
     CDS             5111..5905
                     /locus_tag="PK610_06325"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRJ35583.1"
                     /translation="MKQYHDLLSHILEKGVVKTDRTGTGTISVFGYQMRFDLQEGFPL
                     VTTKKVHLKSIIHELLWFLQGETNIRYLKENGVSIWDEWADENGNLGPVYGHQWRSWP
                     TPDGGKIDQITQLVDMIKKNPDSRRLLVSAWNVAEVNKMALPPCHTFFQFYVANGKLS
                     CQLYQRSADTFLGVPFNIASYALLTMMMAQVCDLQPGDFVHTFGDAHLYSNHIEQAKL
                     QLSRDLRKLPVMKINPSVKDIFGFKFEDFELLNYDPHPHIKAPVAV"
     gene            5907..>6213
                     /locus_tag="PK610_06330"
     CDS             5907..>6213
                     /locus_tag="PK610_06330"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRJ35584.1"
                     /translation="MRISLIVATAPDGAIGKDNKLLWHLPADMKYFRETTSGHCVITG
                     RKNYESIPEKFRPLPNRTNIVLTRNKNCNFPGAISVSSLQEAIKVCQQHNETEAFIIG
                     "
BASE COUNT         1830 a         1184 c         1405 g         1794 t
ORIGIN      
        1 gattcccgtt ctgcggattc atggaataaa attgttccac ctatgtcgcg aaaggcaaag
       61 cttagtcagg aagatgaaag tcttattctc cgttatgtgg taacaatggc aaaaaagaac
      121 tgatcgatta cttcatctga agtttatctc caccctgaat cagatttcct ttttcatcat
      181 aaaattcatg tttggtgtac ccggaggaca tggagtcgta tatggtcact tcttttttct
      241 ttccgcttgg ataaaagctg aaaaaggaaa cgtttttttt atcggtcacg gccgtttttc
      301 cgtcccaaat tgccgtaaag cgcgttatgc tttttttgct ttgataatcg taccattggg
      361 tttgttcgat atcaacagta acgttggttg tatttactgc tgtgacttta cgtaattcaa
      421 cacgactgcc gcgactgtcg taccatacat gttctccaac cggtaagcca ttttcaaaag
      481 tacctgcgta tttattgctt ccgttctcat gccactgacg agctgctccg tggtaaacaa
      541 ctgccccctt ttctccggtg gcaaaatggt attcggccga cttgtgtccg gatgaaaatt
      601 gctcttctgt tctttccaaa atgccattgt tgtaataatt gatcgatttt ctgccattta
      661 caactgcctg catttcattg ttcatttttt tcttcttcag cgcagaaatc cactccactc
      721 tgtactcatt caaacaagca ccggaaaaag gttgaccggt ttggccatct accacaatgt
      781 tttccttggt actgagtgaa gtacaattac aggtagtgcc ctcagttttc gcatcaccta
      841 ccggtaagtc actcgttaag aaggggatag ctgctgtgat tgcaattgct ttgagggaga
      901 atagcatggt gtgtttgatt ttgggacacg aaaataatac ggctatccag gaaataggtc
      961 caaaaatcgc ccaaaagccg gtttttctcc acggagagca ttaaagcccg ttttaacgtc
     1021 gaagtagtgc tctcttaccg gttagagttg aatgatttgc tatctttgca gccccttgaa
     1081 gaagagggtt tttataaaat gatgaatatg aacgtaacac aggaaaagat cgacgatctg
     1141 aacgcggtta tcaaggtgaa actaactccc gaagattatc gtcctaagta tgaagctacc
     1201 cttaaggatt attcccgcaa ggcacaggtt aaaggattcc gtccgggaca tgttcctatg
     1261 gcgatgatca agaagatgta tgggaaatca atccttgcag atgaggttaa caagttgttg
     1321 aatgatagct tgtataatta catcaatgaa aataatctgg aagtattagg taatcctctt
     1381 ccaattgagg agcaggaaag agtagaattt cacgaaaatg ccgaaatgga atttcgttat
     1441 gagattggat ttgctccaaa aattgatttg aatttatcag gattcaaagt tccttatcag
     1501 gctttgaagg tggatgatga aatgctgaat aagcaaattg atgatttgcg cagacgatac
     1561 ggtaaactgg taaatgctga ggaatctatt gccactgata tgcttttcgg gaaattcgaa
     1621 gagcttgatg aaaacggaaa tttaaaagaa ggcggtatta gcaacaccag tacaatttct
     1681 attgagtttc ttaaggatga agccacaaaa aaacaattaa cagggaagaa acctggcgat
     1741 gttgttgatc tggatccccg cactgtttcc cagggcgaat cggatttagc agccatgtta
     1801 ggtgtggagc gcgaagcgtt ggctgatttg cattctaaat tccgatttac cattaatgaa
     1861 atcaaacgca tcgaacttgc cgaattgaat actgagttat tcgacaaatt attcggagaa
     1921 gggactattg ctactgaaga agacttccgt aacagggtat caacagatct caaaaacatg
     1981 tttgcttttg attctgaccg tttgttctta cgcgaggcaa caaagcaatt actcgataac
     2041 ctgagcattg cacttcctga ttctttcctg aagaaatgga ttgttgctac caacgaaaag
     2101 cccattacgg atgagcagtt ggaaaaggat tatccctcgt atgcgcgtgg actgaagtgg
     2161 cagttgatcg aaaacaagat catcaaggac aatgagatca aggttggcaa tgaggaggct
     2221 gtagattata ccaagggcta ccttgtgtca cagtacaatc gttatggatt acctgcacct
     2281 gaagaagacc agctcaacca gagcgcaatg cgtattcttt cgaacaaaga agaggccaag
     2341 cgtgtttatg atgagttgta cagccgtaaa gtcttggctt tcctaaaaga cgctatgaat
     2401 cttgcggtaa ctgagcatgt atatgaggaa ttcatgaaaa tagccggtgc acaggatcac
     2461 aaccatgacc acgagcatga tcatgaccac gagcatgatc acgaacatca ccaccatcac
     2521 tgatttttgt tatttttaac cgaatcaaaa ccattgaacc atgtttccaa aagacgaatt
     2581 ccagaaatac gcggtgaagc ataaaggcat cagcagcctt cacctcgaga aatatattga
     2641 agcatcaatg acaccttaca tcattgagga acgtcagctg aatgttgctc agatggacgt
     2701 attctcacgt cttatgatgg acaggattat tttcctgggt acaggaattt acgatcaggt
     2761 agctaacatc attcaggcgc agcttttgtt tcttgagtcg gttgacgcta aaaaggatgt
     2821 acagatttac atgaattcac ccggtgggtc ggtttatgcc ggtttaggaa tttatgatac
     2881 tatgcaattc atcgcccccg atgttgcgac aatttgtaca ggaatggcgg cttcaatggg
     2941 agctgtactt ttatgtgcag gagctgcagg aaagcgttct gctttgaagc attcgcgggt
     3001 tatgattcac caacctttgg gtggagcaga aggtcaggcg tctgatattg aaatcaccgc
     3061 acgtgaaatc ctgaaactca aaaaggagtt gtacgagatc atttccaagc attccggtca
     3121 accttatgag aaagtttggc aggatagcga tcgtgattac tggatgattg ctgatgaagc
     3181 aaaatcgtac ggtatgattg atgaagtact ggaacgtcac agaaaataat tgaaaactaa
     3241 aaggcccttc ggggcctttt tattccttgt ctgatgaaag agaagaatga agttaagtgt
     3301 tccttttgcg gaagatctaa acgggaaaca aatgttttga ttgccggaat taccggtcat
     3361 atttgtgatc attgtgtttc gcaagcagaa cttattgtca aggaagaagt atcatctcga
     3421 acacaaggag atcttgagca ccaattaaaa ttatcaaagc ctgcagaaat taaaactgca
     3481 cttgatgcat atgtaattgg gcaggatgat gcaaaaaagg tattgagtgt tgctgtttat
     3541 aatcattaca aaaggctgct tcagaaggga aataccaaag aggacgatgt tgagattgag
     3601 aaatcgaacg tgattttagt gggtccaacc ggtacaggaa aaacattgct ggctaagact
     3661 attgcgaaaa tgctgaacgt acctttttgc attgccgacg ctactgtgct tactgaggcg
     3721 ggttatgttg gagaggatgt agagagtata ttatcacgtt tgcttcaggc ggcagattat
     3781 gatgttgctg cggctgagcg tggaattgta tttgtagatg aaatcgataa gatttcccgc
     3841 aagggagata atgcatccat aacacgtgat gtaagtggtg aaggcgtaca acaggcatta
     3901 ctgaaacttt tggaaggttc tgtggtaaat gttccccctc agggaggaag aaaacatccg
     3961 gaacaaaaaa tgattcaggt aaatacacgt aacatattat ttgtttgtgg tggtgctttt
     4021 gacgggatcg acaaaatcat tgcacgccgt gtcaacacac aaaccatcgg ttattctgtg
     4081 gaagaagaag tgcatcgcat cgataaggaa aatatgttga ggtttatcag tccacgtgat
     4141 cttaaatcgt tcggattgat tccggaactt attggtcgtt ttcctgtatt aacctatctc
     4201 gatccgctcg atgccgatgc cttgaggaga atattaacgg agccaaaaaa tgctttgatc
     4261 aaacaatatg taaagttgtt tgaaatggat aatatccgtt tgaagtttga caaggaggtg
     4321 attgatttta tggtcgaaaa agcggttgaa tttaaattgg gagcaagagg attgcggtcc
     4381 atttgtgaag ccatccttaa tgatgccatg tttgaattgc cttccgataa gaacacagag
     4441 acactgcata tcacattgga atacgcaagg gaaaagtttg gcaaatcaaa agtgatgcgg
     4501 ctaaaagtgg cctgatcaga ttgttttttt acccaggatc aacagcgtta cattctttcc
     4561 gtcgatacga gccacatcgg gtagattacc ccattcgcta aatccgaatt ttctgaaaag
     4621 tttcagactt ggttcattgt gagcaaagat gaatccaaga agattttcaa cgccaagcga
     4681 tggcgcagaa tcgattgctt tttggagaaa ggtctctccc agtttctgtc ctctgaaatc
     4741 gctatgaata taaatggcca gttcacaggt gatggaatag gcaggtcttc cgtaaaacga
     4801 gtgaaaactc agccaaccgg caatttttcc gtttttttct gctacccaaa gcggacgggt
     4861 ttgtgcattg tgtttctcaa accattcttt gcgtgattca atagttacgg gttcaagatc
     4921 tgcagttacc atgcgaccgg gaatagtggc attgtagatg gcgactattt cagacaaatc
     4981 atttattgaa gcattacgaa agctgaaatc catttgctgt tgaaaatttc gggtctgcgt
     5041 tcaaaagtaa gcgattatac tcaagggaag agcctcaatg catcgtaatt tcgtgaagta
     5101 aatccattcc atgaaacagt atcatgacct cctcagccac atccttgaaa aaggagttgt
     5161 aaaaaccgac agaacaggca ccggtacaat cagtgttttt ggttatcaaa tgcgctttga
     5221 tcttcaggag ggattccctt tggttactac caaaaaagtt catttgaaat ccattattca
     5281 tgagttgctt tggtttttgc agggcgaaac caatattcgt tacctcaaag agaacggtgt
     5341 gagtatttgg gacgaatggg cggatgagaa tggaaatctt ggtccggttt acggtcacca
     5401 atggcgcagt tggcccactc ctgatggtgg aaaaattgat cagatcacac agttggttga
     5461 tatgattaaa aagaatccgg attcacgccg attgctggtg agtgcatgga atgttgctga
     5521 agtaaataaa atggcgcttc ctccttgcca cacgttcttt cagttttatg ttgcaaacgg
     5581 caaactttcc tgtcagttgt accaacgaag cgctgatact tttttaggtg ttccattcaa
     5641 tattgcttct tacgcattgc taacaatgat gatggcacag gtttgtgatc ttcaacccgg
     5701 agattttgtt cacaccttcg gtgatgcgca tttgtattcc aatcatatcg aacaggccaa
     5761 acttcagtta tcacgcgatt tgcgcaaact tcctgtgatg aagatcaatc cttctgtaaa
     5821 ggatattttc ggattcaaat tcgaagattt cgaattgctg aattacgatc cgcatcctca
     5881 tattaaagca cctgttgctg tttgatatga gaatcagttt gattgtagct acggctcctg
     5941 atggtgctat tgggaaagac aataaattgc tttggcattt acctgccgat atgaaatatt
     6001 tccgcgaaac aacgagcgga cattgtgtaa ttaccggaag gaaaaattac gagtcgatac
     6061 ccgaaaagtt cagacctctc cccaaccgta caaacattgt tttaacacgg aataagaatt
     6121 gcaattttcc cggagcaatt tctgtttctt cattgcagga agctattaaa gtctgccaac
     6181 agcataatga aacggaagca tttataattg gtg
//
LOCUS       DAOMYG010000154         6208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4289_length_6208_cov_7.500894, whole genome shotgun sequence.
ACCESSION   DAOMYG010000154 DAOMYG010000000
VERSION     DAOMYG010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4289_length_6208_cov_7.500894"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PK610_06335"
     CDS             <1..334
                     /locus_tag="PK610_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35585.1"
                     /translation="QQFQKPFHPFFHFVGLDENGKKGGMVSEIVAHQPAKFVSIRHYG
                     FLDGDKEITTGEQVEKWAGGHENYSFEENNGNTTVTVDLDSIEEYLDYFNNTYPAAMD
                     KLKQISEH"
     gene            1061..3175
                     /locus_tag="PK610_06340"
     CDS             1061..3175
                     /locus_tag="PK610_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HRJ35586.1"
                     /translation="MQSFRHELEDLNNPVVEKDIIELERKIRLFREGKIDEEKFRSLR
                     LARGVYGQRQPGVQMIRIKIPYGKLNVAQLRRIADVADEYSNGNLHTTTRQDIQIHYV
                     SLDRTPQLWADLEKDAITLREACGNTVRNITASYLAGIDPNEVFDVSPYAHALFDYFL
                     RHPVGQELGRKFKIAFSSGEKDDAFTFMHDLGFIPKVIIENGEEQRGFKVVLGGGLGA
                     QPVLAKTAFEFLHEDQIIPFTEGVIRVFDRHGERARRHKARIKFLIEEIGLEKFLTLV
                     KEEQKALRVQSFKIDRHKVPVAVLPKNKKAPHVEDLDIDAYDAWLKTNVIEQKQRGYF
                     AVKIRLSTGDFSTTAARQLASLVEDFAGDDIRITINQGLLLKFILPENLPFVFHQLNA
                     IGLAEPGFDSALDITSCPGTDTCNLGITSSYGITRELERVIKSEYPELIYNNDIKIRI
                     SGCMNSCGQHTIANIGFHGSTLKSGQLVVPALQVLLGGGPLGNGEGKISDKVIKVPAK
                     RGPAVLRTLLNDYEEYSEEQEYFNAYYERKGEKYFYDLLKPLASTETILPEELIDWGH
                     DETYKQAIGVGECAGVAIDLVATMLFETEEKLLSAKESFEEGRFADSIYFSYSTLVSG
                     AKAWLVNHGIKTNSQHGIIKDFEIHAEDTFFAGQSFSSIAFAIRDREPDQQFAASYLE
                     QALQFFDQLKQLRQPQIAAQLN"
     gene            3177..3968
                     /gene="cobA"
                     /locus_tag="PK610_06345"
     CDS             3177..3968
                     /gene="cobA"
                     /locus_tag="PK610_06345"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRJ35587.1"
                     /translation="MKTLTHISPQLTLVGAGPGDPELITLKGIRAVQKADVILYDALI
                     HPDILLHASAKAIKVFVGKRAGQHSFTQDEIHRLIAEYALNKGHVVRLKGGDPFVFGR
                     GHEELEFAESLGIPTRIIPGISSSIAVPELQGIPITRRGTNESFWVITGTTRSGKLSS
                     DVALAAQSSATVIILMGMSKLREIRETFEKLGKKNTPFAIIQNGTLANEKNGFGYLHQ
                     IEDIAKKNQLGAPAIIVIGEVVLDHKIFRQEVLHKHYFKSRIAEN"
     gene            4003..4623
                     /locus_tag="PK610_06350"
     CDS             4003..4623
                     /locus_tag="PK610_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HRJ35588.1"
                     /translation="MNQLFPLFLRTDRLHFLIAGGGDVAEEKLQALLRQYPDAHVVVI
                     AEHFETNLVRLAQSHAFVVLRQKAFSASDLNNIQVALAATRDAAFNLSIVQEARKRNI
                     LVNAADMPELCDFYLGAVVKKGNLKIGISTNGSSPILARRLREFFEDVLPDDIDQLIN
                     DLYAVRKSLKGDFQEKSKTLSELTASFRYSGGLLQAARNPDNEVWD"
     gene            complement(5368..5820)
                     /gene="smpB"
                     /locus_tag="PK610_06355"
     CDS             complement(5368..5820)
                     /gene="smpB"
                     /locus_tag="PK610_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRJ35589.1"
                     /translation="MSKVSIRNKKASFEYHLNDVYVAGIMLTGTEIKSIRASKASINE
                     AYCFLHNGELWVKGMHIAEYEPGSYNNHAPLRERKLLLNAREIEKIEKELKVKGTTLI
                     PLEIFLNEKGIAKLKIALATGKKLFDKRDDLKLKDAKREMDRVKKARL"
     gene            complement(5865..>6208)
                     /locus_tag="PK610_06360"
     CDS             complement(5865..>6208)
                     /locus_tag="PK610_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35590.1"
                     /translation="DTRRIEFLLTEFYKNSNEQGLEKVYSLLHPSVRASTDEERFKTF
                     LTTNFTNTGPYIKHSWKSSNLCRNIRPDAIDAYMVVLEVDFENVKTQDIFVLEGTTKG
                     FSILYYYTSIP"
BASE COUNT         1874 a         1310 c         1390 g         1634 t
ORIGIN      
        1 gcaacaattt cagaaaccat tccacccttt tttccatttt gttggactgg atgaaaatgg
       61 gaaaaagggt ggaatggttt ctgaaattgt tgcgcatcag cctgcaaaat ttgtttccat
      121 caggcattat ggttttttgg atggcgataa agaaattaca accggagaac aagtcgaaaa
      181 atgggcgggt ggtcacgaaa attattcttt tgaggaaaac aacggcaaca ccacagtaac
      241 tgtcgatttg gattccattg aggaatacct ggattatttt aacaacacct atcctgctgc
      301 aatggataaa ctaaaacaga tttcggagca ttgatttatt cgcaagtaca attgtttttg
      361 aatccattaa tgataattga tctgcaattt tttatcattg taaatactta aaaaacaatc
      421 acttaaataa tttttacaaa attcttcatc cctgtgtttg gaattgatgg gggaattaac
      481 atagatttgt cgcgatgaaa aacaccaatc tgttcaccat tatgatgcct gcaatgatgc
      541 attgctgctg gtatcgtatt gcctgaacgg aggagaattt catctatagt tagatacaaa
      601 aacgtcaaca tagaaaggcc tccggaaacc cgggggcttt tttttttgat ccacaaccac
      661 cataacaaga aaataaacag aataccaaaa caacaagcat gaaaacgatg aagcaaatta
      721 tgatgtactg tttttgttgt tgccccatgc gcaatatgat gtggcatcat gcccatgtgc
      781 accggaaagt gctttgtccc gatcgaatgc gatgttgacc gcagactatc attaccacga
      841 caaggggctt ttccgggaag gagaagtccc ttttttattt ccaaaacgat cattcacatt
      901 taaaacatac tccaatgcaa ctcacagaat cacaacgtca gcttttctta gcaatatcac
      961 ttcggctttc aaacgtaagc ataaccgaaa aggcaaaacc cgcacgcaag agcagcacga
     1021 aaaaaaacaa accgaatccc attcataacg ccgcctgatc atgcaaagtt tccgacatga
     1081 actcgaggat ttgaataatc cggtagttga aaaagacatc attgaactgg aacgaaaaat
     1141 ccgtttgttc cgcgaaggta aaatcgatga agaaaaattc cgcagtctgc gtctggctcg
     1201 cggtgtgtac ggacagcgtc agccgggtgt tcagatgatc cgcatcaaaa ttccctacgg
     1261 aaaactgaat gtggcacagt tgcgaagaat tgcagatgtg gccgatgaat actccaatgg
     1321 caatttgcat actacaacgc ggcaggacat ccagatccat tatgtaagtc tcgaccgaac
     1381 tccacaactt tgggcagatc ttgaaaaaga tgcaatcacc cttcgcgaag cttgcggaaa
     1441 tacggtgcgg aatattactg catcctattt ggcaggtatc gatccgaacg aagtttttga
     1501 tgtgagtcca tacgctcatg cactgttcga ctattttttg cgtcaccccg ttggacaaga
     1561 acttggaaga aaattcaaga ttgcattttc atcgggagag aaggatgatg catttacatt
     1621 tatgcacgat ttgggattta ttccgaaagt gataattgaa aacggcgaag aacagcgcgg
     1681 atttaaagta gtattgggtg gcggactagg tgcacaacct gttcttgcaa aaacggcatt
     1741 cgaatttttg cacgaagatc agatcattcc atttacagaa ggtgtaattc gtgttttcga
     1801 ccgacatgga gaacgagcaa gaagacataa ggcaaggatc aaatttctca ttgaagaaat
     1861 cggactcgaa aaatttctta ccctggtaaa agaagaacaa aaagcactgc gtgttcagtc
     1921 gttcaaaatt gatcgccaca aagtgcctgt tgctgtccta ccaaaaaata aaaaagcacc
     1981 ccatgttgag gatctggata ttgatgccta tgatgcctgg cttaaaacca atgtcatcga
     2041 gcaaaaacaa cgcggctatt tcgcagtgaa gatccgatta agtacaggtg atttcagcac
     2101 caccgctgca aggcagttag catcgctggt tgaagatttt gcaggagatg atattcgcat
     2161 aacgatcaat cagggattac tactcaaatt cattcttccc gaaaatcttc catttgtttt
     2221 tcatcagttg aacgcgatcg gactcgcaga acccggattc gacagcgcac tcgacattac
     2281 atcctgcccc ggtaccgaca cctgtaatct tggtattacc agcagctacg gcatcacccg
     2341 cgaactggaa cgggtcatca aaagcgaata tcctgaactg atttacaata acgatatcaa
     2401 aatccgcatt agtgggtgca tgaacagttg tggtcagcat accattgcga atatcggatt
     2461 ccatggcagc acgcttaaat caggacagct tgtcgttccc gcgctgcagg tcttgctggg
     2521 tggtggtccg cttggaaacg gtgaaggaaa aatttcggac aaggtgataa aagttccggc
     2581 gaaaagaggt ccggctgtat tgcgaacact gctcaacgat tatgaggagt attcggagga
     2641 acaggaatat ttcaatgcgt attacgaacg caaaggcgaa aaatattttt acgatctgct
     2701 gaaaccattg gcatcaacag aaacgattct tcccgaagaa ctcattgatt ggggtcacga
     2761 cgaaacctac aagcaagcta ttggtgtggg cgaatgtgcc ggtgttgcga ttgatctggt
     2821 agcaacgatg ctttttgaaa cggaagaaaa attactgagt gcaaaagaga gttttgaaga
     2881 aggacgattt gccgacagta tttacttcag ctattccaca ttggtaagcg gggcaaaggc
     2941 atggttggtg aatcacggaa tcaaaacgaa ttcgcagcat ggaattatca aggactttga
     3001 aattcatgca gaggatactt tttttgccgg acagtccttt tcctccatcg cttttgctat
     3061 tcgtgatcgt gaaccggatc aacaatttgc tgcaagttat cttgagcagg cacttcaatt
     3121 tttcgatcaa cttaaacagc tgcgtcaacc acaaatagca gcacaactga attaacatga
     3181 aaacacttac acacatatcc ccgcaactaa cactggtagg cgcgggaccg ggagatcctg
     3241 agctgatcac gctaaaagga attcgtgcgg tacaaaaagc agatgtcatt ttatacgatg
     3301 cactgatcca tccggatatc cttttacatg catcagcaaa agcgatcaaa gtctttgttg
     3361 gcaaacgcgc aggtcagcac agcttcacgc aggatgaaat tcaccgactg attgcagaat
     3421 acgcattgaa taaaggacat gtagtacgac tcaaaggcgg agatcctttt gtatttggca
     3481 gaggtcatga agaactggaa ttcgcagaat cgctcggaat acctacacgc atcattccgg
     3541 gaatcagttc atcgattgca gttccagaat tgcaaggaat tccgataacc cgcagaggaa
     3601 ccaatgaaag tttctgggta atcaccggaa caacacgcag cggaaaatta tcgtcggatg
     3661 ttgccttggc cgcacaatcg agtgctacgg tgatcatact gatgggcatg agtaaactcc
     3721 gggagatccg cgaaacattt gaaaagctgg gcaaaaaaaa tacgccattt gccattattc
     3781 agaacggaac attagcgaat gaaaaaaacg gattcggcta tttgcatcag atcgaagaca
     3841 tcgcgaaaaa aaaccaattg ggtgcaccgg ccattattgt aattggcgaa gttgtgctcg
     3901 atcacaagat cttcaggcag gaagtcctgc acaaacatta tttcaaaagc agaattgcgg
     3961 aaaattaatt tccccgctgc taaaaccgaa aaaaaaagac tcatgaatca gttattcccc
     4021 ttatttcttc gcaccgaccg tcttcatttt ctcatagcag gaggaggcga tgttgccgaa
     4081 gaaaaattac aagccctatt gcgccaatac cctgatgccc atgtggtggt tattgcagaa
     4141 catttcgaaa caaacctggt gcgactcgct caatcgcatg catttgttgt gttgcgacaa
     4201 aaagcgtttt ctgcaagtga tctgaacaat atacaggtag cgcttgcagc cacccgcgat
     4261 gctgcattta atttatcgat tgtacaggaa gcacgaaaac gaaacattct ggtgaatgcc
     4321 gccgatatgc ccgagttgtg cgatttttat ttaggtgcag tcgtgaaaaa aggaaatctg
     4381 aaaatcggta tttccaccaa cggctcctct cctattctgg cgcgacgact ccgtgaattt
     4441 ttcgaagacg tgttacccga tgatatcgat cagctgatca acgatcttta tgctgtgcgt
     4501 aaatcgctga aaggagattt tcaggaaaaa tcaaaaacac tcagtgagct tactgcatcc
     4561 ttccggtatt cgggaggctt attgcaagct gcgcgaaatc cggataatga ggtgtgggat
     4621 taaaggaacg ggaacgggaa cggggacggg aaagggaacg gggacgggaa cgaaagcgag
     4681 aacaaggaca gtgaaccgca aagaaaatgg gttgggtggg ttgataattc caaaattgta
     4741 gtccattcaa catcttgagt tacatcatga ttttttaagt ggagcaaagg cacctacggt
     4801 gctgcgctaa ggtagtacca ttcatgtttg tcctgaaaat tgaaaatggt gtattattaa
     4861 ttatagcaca aactcaattt ttcagcagga actcagatgg aattccttcg cgcaatgccg
     4921 aaggcatctt tgctccactt gaaaaaacat gtaatgttag actgtttaaa atgaaaatcc
     4981 ggcttcatca ttatcctcaa tcaaaacaaa aaaccctttg cggtaaaacc ttagtgactc
     5041 tcggtgccct aagtgtctaa gtggttagaa aaaaatttat caggtacacc ttctcgcaat
     5101 gccgaaggca tctttgctcc acttgaaaaa aatccaaaat ggtagaaccg taaaaatagt
     5161 agactacata aaaccttttt cacaaatcaa gacaattccc tttgcggttc cctttactcc
     5221 tgccataggg aaatgcccaa cccggtctat cggccccata agggttaccc atattgtgaa
     5281 agaggcctcc ataggcgatt gaaaattgga aattagtaat tgaaaatccc gacgctctcc
     5341 taaagttcgg tcggttcagg ttggaagtca caatctcgct ttcttaacgc gatccatttc
     5401 gcgtttggcg tctttcagtt tgagatcgtc gcgtttgtcg aatagttttt ttcctgtggc
     5461 gagagcgatt ttcagtttgg cgatgccttt ttcattgaga aaaatttcga ggggaatcaa
     5521 agtggttcct ttcactttca attctttctc aattttttcg atctcgcgcg cattgagcag
     5581 aagtttgcgt tcgcgcaggg gtgcgtggtt gttgtaacta ccgggctcgt attcggcaat
     5641 gtgcatgccc ttcacccaca attcgccatt gtgcagaaaa caatacgctt cgttgatact
     5701 tgccttgctt gcacggatcg atttgatctc ggtaccggta agcataattc cggctacgta
     5761 cacatcattc agatggtact caaacgaagc tttcttattg cggatactga ccttgctcac
     5821 ggtgcaaagt tagcaaatta gcggtacagt ggctgtgctg agtgttatgg aatgctggtg
     5881 taatagtaga gaatggaaaa gcccttggtg gttccctcca gaacgaaaat atcctgtgtt
     5941 tttacattct cgaaatcgac ctccaatacc accatgtaag catcaatggc gtcgggacga
     6001 atattcctgc agaggttgga acttttccag ctgtgtttga tgtacggacc ggtattggtg
     6061 aagttggtcg tgaggaacgt tttaaatcgc tcttcatcgg tagaagcgcg aaccgaagga
     6121 tgcagcaggg aatacacttt ttccaatcct tgttcattac tgtttttgta aaattcggtg
     6181 agcagaaatt cgatgcgacg cgtatcat
//
LOCUS       DAOMYG010000155         6185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4313_length_6185_cov_8.175041, whole genome shotgun sequence.
ACCESSION   DAOMYG010000155 DAOMYG010000000
VERSION     DAOMYG010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4313_length_6185_cov_8.175041"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PK610_06365"
     CDS             <1..409
                     /locus_tag="PK610_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HRJ35591.1"
                     /translation="PNPAELIINGKLDSILEELKRIYDIVIVDLPPVGLVTDGIPIVQ
                     MADYPIYVFRANYSKKNFVQNADRLVNENNIQRLSVVLNSVDTDRGSSGYNYGYGYGY
                     GYGYGYTYGGNYYTEGTNATEKKIRFFRRKNKK"
     gene            406..960
                     /gene="rfbC"
                     /locus_tag="PK610_06370"
     CDS             406..960
                     /gene="rfbC"
                     /locus_tag="PK610_06370"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRJ35592.1"
                     /translation="MKIVSEPLPGLFLLEPSVFGDERGYFFESWNHGKFCELIQKEVH
                     FVQDNQSLSGKGVVRGLHLQAPPFAQGKLVRVVRGTVLDIALDIRKNSPTYGKHFSAE
                     LSEKNHRMMWIPEGFAHGFSVLEDQTIFLYKCTNFYNKASEHCIRYNDSALNINWKTE
                     QAIVSEKDKEGLLLSDFESPFTYV"
     gene            953..2050
                     /locus_tag="PK610_06375"
     CDS             953..2050
                     /locus_tag="PK610_06375"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRJ35593.1"
                     /translation="MSDQEYYTGIFILVFSVFIALTVFINGVLLKFVKTLGIREQSTT
                     IIRWSPVSKPSLGGITFFLIFLLSISSFSFFFPGNIIFHDNHSLALIIICILGFLLGL
                     GDDAYNTNPLLKLFVQTLCGVVLWLSGAGIKITPYEWLNITLTIIWVIGVMNSINMLD
                     NMDGITASVSASIILSFILIQIVSDKIHLFDMFVLCAVLASVIGFLFHNWNPSRMFMG
                     DTGSQFLGVFLAYYGIRYFWNGTDIQSSLIQSKQITLVLIGFMIPILDSSIVIINRLL
                     RGQSPAVGGKDHTTHHLSYLGYSDSQVGFIFLGLSAFSTLLILVMVRLIDGWSHLYTI
                     AFWCWFFVVFCVLFIITRKNKNYVSQGKQGQ"
     gene            2022..3002
                     /locus_tag="PK610_06380"
     CDS             2022..3002
                     /locus_tag="PK610_06380"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRJ35594.1"
                     /translation="MSAKESKGSNRLRNIAFFSIIACCVIVAMRALTFSVNIPAEKNN
                     DTLYREHVAENYKIFSLATPDKMEFAGERVPLEDIDIRERLDKELTINTYWHSATMFM
                     IKRSNRWFPTIERILREEGIPEDFKYIAAIESNFDHVVSPAGASGFWQFLRATGQSYG
                     LEVNGFVDERYNLEKATRAACKYFKDAYAVLGNWTLVAASYNMGVGGVQSKVKEQQSK
                     NYYDLYLNSETHRYVFRALAAKEILSKPADYGFNVRPKDLYPAYETKTIEIDSSISDL
                     KSFAQTQGTNLKILKLLNPWLRDSSLPNKVRKKYALLLPKEDFALKPVAP"
     gene            3017..5848
                     /gene="uvrA"
                     /locus_tag="PK610_06385"
     CDS             3017..5848
                     /gene="uvrA"
                     /locus_tag="PK610_06385"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRJ35595.1"
                     /translation="MSKQSIEEESISVYGARVHNLKNVDISIPRNKLVVFTGLSGSGK
                     SSLAFDTIYAEGQRRYIETFSSYARSFLGNLERPDVDKITGLSPVISIEQKTVSKNPR
                     STVGTITEVYDFLRLLFARAGEAYSYVTGEKMVKYSDSQIQELILTDFNKKKIAILAP
                     VVKGRKGHYRELFEQIKKMGFLRVRVDGKIKELTERISLDRYKIHDVEIVVDRIEVKA
                     DNEQRISESIKTAMKHGKGSLMILDMEDDSTRYFSRSLMCPTSGISYPEPAPNLFSFN
                     SPYGACPKCDGIGEVTEADLSKIIPDPSKSIKQGAIAPLGEFKNALILRQIEGLLAHH
                     KLKITTPVDEIPESVISKVLYGTDEIFKLPNATGITEQVSNYEGVVTFVLRHSQENPS
                     KTIQRWASGFMNQVKCKECNGSRLKKESLYFRIAGKNIHELASLDLYQLKDLFDSIEK
                     KLSARQLKIATEILKEIRSRIGFLLDVGLNYLTLDRSARSLSGGEAQRIRLATQIGSE
                     LVGVLYILDEPSIGLHQRDNTRLIQSLKKLRDIGNSVIVVEHDKEMIMESDFVVDIGP
                     GAGINGGKIVEATEPSRLKKGDSITTAYLTGKKGIPVPKNRRPYSGKNLLLKQASGHN
                     LKNVTIELPLQQLVCITGVSGSGKSSLINETLYPILSRHFYNSVQTPLPYKKIEGLEH
                     IDKVIEIDQSPIGRTPRSNPATYTNMFNDIRNLFAMLPEAKVRGYSPGRFSFNVKGGR
                     CETCQGGGLRTIEMNFLPDVYVECETCGGKRYNKETLEVRFKGKSISDVLNMTISNAV
                     EFFEGIPAIRQKLVTMESVGLGYLTLGQPSTTLSGGEAQRIKLSTELSKRETGRTLYI
                     LDEPTTGLHFEDIRLLLDVLNRLVDNGNSVLVIEHNLDIIKVADHVIDMGPEGGSGGG
                     EIIAMGTPEQVALVKGSHTGKYLKAELKG"
     gene            5967..>6185
                     /locus_tag="PK610_06390"
     CDS             5967..>6185
                     /locus_tag="PK610_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRJ35596.1"
                     /translation="MTDQEKKTHEKFVLKNWSDIKSENSWTIFKVMSEFVEGYEKLAK
                     IGPCVTIFGSARTKPEQNYYQLAEKIAFQ"
BASE COUNT         1854 a         1291 c         1286 g         1754 t
ORIGIN      
        1 accaaaccct gcagaattga tcattaacgg aaagctggat tccatactcg aagaacttaa
       61 gcgaatttac gatatcgtta tagttgatct tcctccggtt ggacttgtta ccgatggtat
      121 tccaattgta caaatggccg actatcccat ttatgttttc cgtgcgaatt attcaaaaaa
      181 gaatttcgtg cagaatgctg atcgacttgt gaatgaaaac aatattcagc gtttatcggt
      241 agtattgaac agtgtggaca ccgaccgagg ttcttccggc tacaattacg gatatggtta
      301 tggatatggt tatggatatg gatacacata tggcgggaat tattacactg aaggaacgaa
      361 tgcaactgaa aaaaaaatcc ggtttttccg gagaaaaaac aaaaaatgaa aattgtatct
      421 gaaccgctac ccggattatt tttgcttgaa ccatcggtat ttggtgatga gcgcggttac
      481 ttttttgaat cctggaacca tggtaaattt tgtgagctga ttcaaaaaga agttcatttt
      541 gtacaggata atcaatcctt atccggcaag ggtgttgttc gcggtttaca tcttcaggcg
      601 ccaccatttg cacaaggcaa actcgttcgt gttgttcgtg gtaccgttct tgatattgcc
      661 ttggatattc gtaagaattc gcctacttat ggaaagcatt tttcagcaga gctttcggaa
      721 aaaaaccatc gcatgatgtg gattcccgaa ggatttgcac atggattttc tgttttggaa
      781 gaccagacca ttttccttta caaatgcacc aacttttaca ataaagcatc tgaacattgt
      841 attcgttata acgattctgc cttaaacatc aattggaaaa cagaacaggc cattgtttcg
      901 gaaaaagata aagaaggatt gttgttgtcg gattttgaat ctccttttac gtatgtctga
      961 tcaggaatat tatactggaa ttttcattct tgtattctca gtatttattg cacttactgt
     1021 ttttatcaat ggggttttgc tgaagtttgt taaaacactc ggcatacgcg aacaatccac
     1081 aaccattatc cgctggagtc cggtttcaaa accttctctt ggtggaatta cattttttct
     1141 cattttcctt ttatcgatat cgagtttctc ctttttcttt cccgggaata tcatttttca
     1201 cgataatcac tctctggccc tgatcatcat ttgcattctg ggtttcctgc ttggactggg
     1261 agatgatgca tacaatacga atccgcttct taaactattt gtacaaacgc tttgcggagt
     1321 tgtactttgg ttaagtggtg ccggaataaa aatcacccct tacgaatggc tgaatatcac
     1381 tttaaccatc atttgggtta tcggcgtaat gaattcgatc aacatgctcg ataatatgga
     1441 cggtatcact gcttccgtat cggcatcaat aattctcagt tttattttga ttcagattgt
     1501 aagcgacaaa attcatttgt tcgatatgtt tgttttatgt gctgttctcg cctctgtgat
     1561 cggatttctc tttcacaact ggaatccctc acgcatgttt atgggggata ccggaagtca
     1621 gttccttggt gttttcctgg cgtattatgg cattcgttat ttctggaacg gcaccgatat
     1681 ccaaagtagt ttaattcaat ccaaacaaat taccctggtg ctgattggct ttatgattcc
     1741 aatcctcgat tcgagtatag tgatcattaa ccgattactc cgcggacaat cgcctgctgt
     1801 tggtggtaaa gatcatacca ctcatcattt gtcgtacctg ggttattccg actcgcaggt
     1861 tggatttatc tttttgggat taagcgcctt ctccaccctt ttaattctgg taatggtacg
     1921 cttaatcgat ggttggtcac acttatacac tattgctttc tggtgctggt tttttgttgt
     1981 attttgtgta ttattcatta taacccgtaa aaacaaaaat tatgtcagcc aaggaaagca
     2041 agggcagtaa tcgcttacgg aatattgcat ttttctcaat cattgcctgt tgtgtcattg
     2101 tagcaatgcg tgcattaacg ttttcggtaa acatacccgc agaaaaaaac aacgataccc
     2161 tttaccgcga acatgtagcg gagaactaca agatcttttc gcttgccact cccgataaaa
     2221 tggaatttgc aggagagcgt gttccgcttg aggatattga tattcgcgaa cgactggata
     2281 aggaactcac cattaacacc tactggcact cagcaacgat gtttatgatc aaacgatcga
     2341 accgctggtt tccaactatt gaacgcatac tccgtgaaga aggaattcct gaagatttta
     2401 aatacatcgc ggccattgaa agcaatttcg atcatgtggt ttctccagca ggggcatcgg
     2461 gattctggca atttcttcgt gcaacaggac aaagctacgg gctggaagtg aatggttttg
     2521 ttgacgagcg ctacaacctc gaaaaagcga ccagagcagc ttgtaagtat tttaaggatg
     2581 catatgcagt tctcggtaac tggaccttgg ttgctgcttc ttataacatg ggtgttggag
     2641 gagttcaatc caaagtcaag gaacaacaat ccaaaaatta ttatgatctg tatctgaatt
     2701 ccgaaaccca ccgatatgta ttcagggcat tggctgctaa agaaatttta tctaagcctg
     2761 cagattacgg atttaatgtt cgtccaaagg acctctaccc tgcctacgaa acaaaaacca
     2821 ttgagatcga cagcagtatc tctgatctga aatcttttgc ccaaacgcaa ggaacaaacc
     2881 tgaaaatcct gaagctgctc aatccatggc tgagagattc ttcgctgccc aataaagtga
     2941 gaaaaaaata cgcattgcta cttcctaaag aagatttcgc tcttaaaccg gtagcgccct
     3001 aagttgttat aataccatgt cgaaacaatc cattgaagaa gaatccattt ccgtatacgg
     3061 tgcccgcgtt cataatctga aaaacgtcga catctccatt ccccgtaaca aactggtcgt
     3121 ttttaccgga ctaagcggaa gcggaaaatc atcgcttgcc ttcgacacca tttacgcaga
     3181 gggacaacgt cgctacatcg aaaccttttc ttcgtatgcg cgatcgttcc ttggcaacct
     3241 ggagcgacct gatgtagaca aaataaccgg attaagtcct gttatctcca ttgaacaaaa
     3301 aacagtaagt aaaaatccgc gatcgaccgt tggaaccata actgaggttt acgatttttt
     3361 gcgactcctt ttcgccagag ccggtgaagc gtacagctat gttaccggag aaaaaatggt
     3421 gaagtactcc gacagtcaga ttcaggagct catccttacc gatttcaaca agaaaaaaat
     3481 tgccattctt gctcccgttg taaaaggaag aaaaggtcat taccgcgaac tttttgagca
     3541 gatcaaaaaa atgggctttt tacgggtgcg cgtcgatgga aaaatcaagg agctaacaga
     3601 acgaatcagt ctcgatcgct ataaaattca cgatgtagaa attgttgtgg atcgtataga
     3661 agtgaaagcc gacaatgaac aacgcatcag cgaatccatt aaaacagcaa tgaaacatgg
     3721 caagggatca ctaatgatcc tcgacatgga agatgacagc acacgctatt tcagtcgctc
     3781 actgatgtgt ccaacttcag gaatttctta tcctgaaccg gctccaaatc tcttttcatt
     3841 caactcgccc tatggcgctt gtccgaaatg cgacggaata ggagaagtaa cggaggcaga
     3901 cctcagcaag atcattcctg atccttcaaa aagcattaaa cagggagcaa ttgcgccact
     3961 tggtgaattt aagaacgcgt tgattcttcg tcagatcgaa ggtttacttg ctcatcacaa
     4021 actgaaaatc acaacacctg ttgatgaaat tcctgagtcg gtgatcagca aagtgctcta
     4081 cggaactgac gaaattttca aattacccaa tgcaacaggc ataaccgaac aggtgagcaa
     4141 ttatgaaggt gttgtaacct ttgtattgcg acactcgcag gaaaaccctt ctaaaaccat
     4201 tcagcgctgg gcttccggat tcatgaatca ggtaaaatgc aaagaatgca atggcagtcg
     4261 tctgaaaaaa gaatccctgt attttcgcat tgcaggaaag aacatacatg aactcgcttc
     4321 acttgatctt tatcaactga aagatctttt cgattcgatt gaaaagaaac tttcagcgcg
     4381 gcaacttaaa atcgcaaccg aaatcctcaa ggaaatccgt tcacgaatag gttttcttct
     4441 ggatgttgga ctcaattacc tcaccctcga ccgaagcgca cgctcactca gcggtggtga
     4501 agcgcaacga atcagacttg caacgcagat tggatcggaa ctggtgggtg tattgtatat
     4561 tctggatgaa ccttcaatcg gacttcacca acgcgacaat acccggttaa ttcaatcctt
     4621 gaaaaaactt cgtgatatcg gtaactctgt tattgtggta gaacacgata aggaaatgat
     4681 catggaatcc gatttcgttg tggatattgg tcccggcgca ggaattaacg gtggaaaaat
     4741 cgttgaagcc actgaacctt cgcgattaaa aaaaggagat tcaatcacca ctgcctatct
     4801 caccggaaaa aaaggaattc ccgttccgaa aaatcgtcgg ccttattccg gaaaaaactt
     4861 attgctgaaa caggccagcg gacataattt aaaaaatgtc accattgaat tgcctttgca
     4921 gcaacttgta tgtatcacag gtgtatccgg aagcggaaaa tcgagtttga tcaacgaaac
     4981 gctttacccg attttaagca ggcatttcta caactcggtg caaacaccat tgccctataa
     5041 aaaaattgaa ggactcgaac atatcgataa agtaatagaa attgatcaat cgcctatcgg
     5101 acgcacaccc cgatccaatc ctgctacgta tacaaacatg ttcaatgata tccggaactt
     5161 atttgcaatg ttacccgaag caaaagttcg cggttacagt cccggcagat tctccttcaa
     5221 cgtaaaaggt ggccgttgcg aaacttgtca gggtggtgga ttgcgaacaa ttgaaatgaa
     5281 ttttcttcca gacgtatatg tagaatgtga aacctgcgga ggaaaacgct acaataaaga
     5341 aacactggaa gtacgattta aaggaaaatc aataagcgat gttctaaaca tgaccatctc
     5401 gaatgctgtt gaatttttcg aaggtattcc agcgatacgg caaaagttgg tcacaatgga
     5461 atccgttgga ttgggttatc tcaccttagg acagccgtcc actacacttt ccggaggaga
     5521 agctcagcga attaaactct caacggaatt atccaaaaga gaaaccggaa gaacacttta
     5581 cattttggat gaacctacaa ccggattgca tttcgaagat atccgcttgt tgctggatgt
     5641 attgaatcgt ttggtggaca acggaaattc agttctggtt atcgaacaca atctggatat
     5701 cataaaagtt gcggaccatg ttattgatat gggtcccgaa gggggaagtg gtggtggtga
     5761 aattattgcc atgggtactc cggagcaggt tgcattggta aaaggtagtc ataccggaaa
     5821 atacctgaaa gcagaattaa aaggataatt ccttatttcc gttacctgtt acaaaactac
     5881 cgtaaaccgg ttgtcataac cattttggtt tttggccgta caatggaaca gattttgatt
     5941 atacatcatt gctacaccct atcaccatga cagatcagga aaagaaaaca cacgaaaaat
     6001 tcgttcttaa gaactggagc gatattaaat cagaaaacag ttggaccatt tttaaagtga
     6061 tgtctgaatt tgttgaaggc tatgaaaaac tcgcgaagat tggtccctgc gtaaccatat
     6121 tcggttcagc gcgcaccaaa ccggagcaga actattacca actcgcagaa aaaattgcat
     6181 ttcag
//
LOCUS       DAOMYG010000156         6170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4318_length_6170_cov_7.703679, whole genome shotgun sequence.
ACCESSION   DAOMYG010000156 DAOMYG010000000
VERSION     DAOMYG010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4318_length_6170_cov_7.703679"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PK610_06395"
     CDS             complement(<1..636)
                     /locus_tag="PK610_06395"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRJ35597.1"
                     /translation="MSISRRKFLPLLGAAALSPSLMSFSEETQTDTISKILPPALKKG
                     ATIAVTSPAGALRDLSTATKFKSKLQSLGFNVVMGETVNAKFGYLAGEDDLRVNELQH
                     FFSDPAIDAIVCMKGGYGCMRIIDKLDYALIRKNPKIFMGFSDITALLNAVYLKSGVV
                     TFHGPVGNSSWNDFSLLHINSVLMHGHQIIYRSPAKPEDEVKVVRAGKAEGI"
     gene            complement(649..2433)
                     /locus_tag="PK610_06400"
     CDS             complement(649..2433)
                     /locus_tag="PK610_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015361660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HRJ35598.1"
                     /translation="MRRLTALLFSALLLASCSKPAETDPGKLLSRKWIDEKDTTQFLL
                     FNVLPDGKQSVSGNVKGIQNEPLSGTWNLKGAELKLILVRSSETAIPLDSAVFYSGPA
                     GSEVKFYNNNNPVTRMDASGSSDLLLERLFFIDTLSANQLVLHNDAGFAAEFEYTPQV
                     YNPPFSLESLLRGLIGLMALVIITWVFSENRSKVNWRLVGIGLTLQIVFAIGVLKVPF
                     VESMFEGISAFFIKVINFTQEGTDFLFRSFVSGKIESPLANFVVKVLPTVIFFSALTS
                     LLFYWGILQKVVYGLAWVMRKTMRLSGAESLAAAGNIFLGQTEAPLLVKPYIGSMTRS
                     ELLCLMTGGMATIAGGVLAAYVGFLGGDDPEQQLYFAKHLLAASVMSAPAAIIAAKIL
                     LPETESFNTEMKIPRDRIGTNALEAITNGTSDGLRLAANVAAMLLVFIALIAMGNFVM
                     EEIIGEYTGLNAIIRENTVYSGLSLQFFVGYIGSPIAWIMGVPSEDTILVGQLLGEKT
                     ILNEFYAYTSLGELKAAGKFHHEKSIVMATYVLCGFANFASIGIQIGGIGSLAPNRKS
                     ELSKLGFRALLGGTMACLLTAVVVGMFL"
     gene            complement(2437..3048)
                     /locus_tag="PK610_06405"
     CDS             complement(2437..3048)
                     /locus_tag="PK610_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRJ35599.1"
                     /translation="MEKIKLEIVGLSYSQTQSGAYALVLGEAGGKRRLPIIIGGFEAQ
                     AIAIELEKMTPSRPLTHDLFRSFCERFAVNVKEVIIYNLVEGIFYAKVMCEGEDGKTA
                     EIDARTSDAIALAVRFSCPIYTYEFILSSAGIILDEEAVRSTADEDDEIDEEMDLGEL
                     THPADFSKMSVNELEKGLQEAIDNEDYERASRIRDELSNRKNN"
     gene            complement(3124..4089)
                     /locus_tag="PK610_06410"
     CDS             complement(3124..4089)
                     /locus_tag="PK610_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRJ35600.1"
                     /translation="MSVLIFADTSNGKVNKNALEAASYGAKVASQLGTTATVITYGAT
                     ANDALAAFGEQGVSKVLVNRSLSGADSQQVTRLVLAAAEKEASDLIVFSHDLLGRAVS
                     PRVSARLNAGLVAGAIDYPETSGGFSVKVNLFSGKAYGFIEVHSAKKVISILPNSLGL
                     ANAGGSAVVEDFAADCGTAAVCVKEVKKNEGEIALPEAEMVVSAGRGMKGPEHWGIIE
                     ELAKELGAATACSRPVADIGWRPHHEHVGQTGIAIAPNLYIAVGISGAIQHLAGVNRS
                     KVIVVVNTDPEAPFFKAADYGIVGDAFQVVPQITEALKKHKAAQH"
     gene            complement(4113..4853)
                     /locus_tag="PK610_06415"
     CDS             complement(4113..4853)
                     /locus_tag="PK610_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRJ35601.1"
                     /translation="MKILVCISKAPDTTTKIAFSDNDTKFNETGVQYIVNPYDEWYAL
                     VRGLELTENSGGSVTIITVGTTADEPVIRKALAIGANEAVRIDAEATDAYFVARQIAE
                     YAKGKGFDMVLLGKETIDFNGSQVGGMVAELLDLPYVSIASKLDVNNNVATIERDVQG
                     GTEVVEANLPLVVSCAKGMAEQRIPNMRGIMAARTKPLTVVPPAGADRLTSVKKFAMP
                     AAKSACKMIPADHAGQLIELLHNEAKVI"
     gene            5133..6113
                     /locus_tag="PK610_06420"
     CDS             5133..6113
                     /locus_tag="PK610_06420"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HRJ35602.1"
                     /translation="MREVQFREALREAMNEEMRRDERVFLMGEEVAEYNGAYKVSQGM
                     LDEFGPKRVIDTPIAELGFAGIGVGAAMNGLRPIVEFMTWNFAILAADQIINSAAKML
                     QMSGGQYNVPIVFRGGNGTAGQLAATHSQSFEAFYAHVPGLKVITPSNPYDAKGLLKA
                     AIRDNDPVVFLESEKMFGDKGMIPEGEYVLPIGVADIKRKGKDVTIVSFGKIMKVALS
                     AAEELAKEGIEAEVIDLRTIRPIDYDTVIESVKKTNRLVVVEESWPLASISSEIAYWV
                     QRYAFDHLDAPVWRVTQSDTPFPFATSLMAEALPNNERVIRAVKEVLYKF"
BASE COUNT         1773 a         1511 c         1267 g         1619 t
ORIGIN      
        1 aatgccttca gcctttcccg cacgaacaac tttcacttca tcttccggtt ttgccggtga
       61 gcggtaaatg atttgatgtc cgtgcataag cacagaattg atatgcaata agctgaaatc
      121 gttccaggag gaatttccaa ccggaccatg aaatgtaact accccacttt ttagataaac
      181 tgcattaagc aaagcagtga tatcggaaaa gcccatgaaa attttcgggt tcttccttat
      241 gagtgcataa tccaatttat caatgatccg catgcagcca taaccacctt tcatgcatac
      301 aatcgcatca atcgccggat ccgaaaaaaa atgctgcaat tcattcaccc tcaaatcatc
      361 ctcaccggcc aggtagccga attttgcgtt taccgtttca cccattacca cattaaatcc
      421 gagcgactgc aatttacttt tgaattttgt ggcggtggaa agatcgcgta atgctccagc
      481 aggagaagta acagcaatag ttgctccctt ttttaatgca ggtggaagga ttttcgaaat
      541 agtatccgtt tgcgtctcct ctgaaaaact catcagcgaa ggactaagtg ctgcagctcc
      601 cagaagtggc aaaaatttac gtcgggagat actcatttcc ggttcgtttt ataaaaacat
      661 tcccactact acagcggtga gtaaacatgc catggtacca cccagcaatg cgcggaatcc
      721 aagtttcgat aattcagatt tacggttagg cgccaatgat ccaataccac caatctgaat
      781 cccaatagag gcgaaattgg caaaaccgca caatacatag gttgccatca caatcgattt
      841 ttcatgatga aattttcccg cggccttcag ctcaccaagc gaagtatagg cataaaattc
      901 attgaggatc gtcttctctc cgagtaactg tcccacaaga atggtatcct cagaaggcac
      961 acccatgatc catgcaatag gggaaccaat atagcccaca aaaaactgca aagacaaacc
     1021 actatataca gtgttctcgc gaatgatggc attgagtccg gtgtattcgc caatgatctc
     1081 ttccatcaca aaattaccca ttgcaatcag cgcgataaaa acaagcaaca tagccgccac
     1141 atttgcagca agtcgcaatc cgtcggaagt tccgttggtg attgcttcca gtgcattggt
     1201 gccgatgcga tcgcgtggaa ttttcatttc cgtattgaag ctttccgttt cgggcagcaa
     1261 aattttagca gcaatgatcg ccgccggtgc cgacattacc gaggctgcaa gcaaatgttt
     1321 ggcaaaatac aattgctgtt ccggatcatc gccaccgaga aaacctacat aggcagcgag
     1381 tacaccacct gcaatagttg ccattcctcc tgtcatcaga cacaacaatt ccgaacgcgt
     1441 catactgccg atatatggct tcaccagcaa gggtgcttcg gtctgaccaa gaaaaatatt
     1501 tcctgcagcg gccagacttt ctgctcctga taaacgcatt gtttttcgca tcacccaagc
     1561 caaaccataa accacttttt gcaaaatccc ccaatagaac aacaaactcg taagtgcaga
     1621 aaagaaaata accgttggta aaaccttcac cacaaaattg gcgagcggac tttctatttt
     1681 tccactcaca aaacttctaa aaaggaaatc agtcccctcc tgtgtgaaat tgatcacctt
     1741 aataaagaaa gcagaaatgc cttcgaacat tgattccaca aaaggaactt ttaatactcc
     1801 tattgcaaaa acaatctgca gtgttaaccc gatacccacc aatcgccagt tcactttact
     1861 tctgttttca ctgaacaccc aggtaatgat caccagcgcc atcaaaccga tcagtccgcg
     1921 taacaaagat tccaacgaaa atggcggatt gtacacttgt ggtgtgtatt caaattcggc
     1981 agcaaagcct gcatcattat gtaaaaccaa ttgatttgcg ctaagtgtat caataaaaaa
     2041 caagcgctcc aataaaagat ccgacgaacc cgaagcatcc attcgggtaa caggattatt
     2101 gttgttatag aatttcacct ccgaaccggc aggaccggaa taaaaaactg cggagtcgag
     2161 cgggatggcg gtttcagatg atcgaactaa aatcaatttc agttctgcac ccttgagatt
     2221 ccatgtcccg gataaaggtt cattctgaat tccttttaca tttccggaaa cgctttgttt
     2281 tccatccggt agtacattaa aaaggaggaa ttgcgttgtg tctttttcat cgatccattt
     2341 acgcgaaagt aattttccgg gatcggtttc agcaggttta ctgcaggagg ccagcagcaa
     2401 tgcactaaag aggagcgcag tgagccggcg catagcttaa ttatttttcc ggttggaaag
     2461 ttcgtcgcga atgcgggatg cacgctcgta atcttcattg tcgattgctt cctgcaagcc
     2521 cttctcgagt tcattcaccg acatcttcga gaaatcggca ggatgggtca attcacccag
     2581 atccatttcc tcgtcaattt catcatcctc atcggcagta ctgcgtaccg cctcttcatc
     2641 cagaatgata cctgctgaag ataaaatgaa ttcgtaggta tagatcggac aagaaaaccg
     2701 tactgcaagc gcaatagcat cagaggtacg tgcatcaatc tccgccgttt tcccatcttc
     2761 accttcacac atcaccttag cgtaaaaaat gccttcaaca agattgtaaa tgatcacttc
     2821 tttcacgttt acggcaaaac gctcgcaaaa ggaacgaaaa agatcatggg taagcggacg
     2881 actgggcgtc attttttcaa gttcgatggc gatggcctga gcttcaaatc cgccgataat
     2941 gatcggtaaa cgtcgcttcc ctcctgcttc acccagtaca agcgcatagg cacctgattg
     3001 ggtctggcta tatgataatc ctacgatttc gagcttgatc ttttccatgt aaaaaacaaa
     3061 cagggaagca aatgcttccc tgtaaatccg agtataaaga tattaaaaaa aatcagtgca
     3121 aaatcaatgc tgcgctgcct tgtgtttctt taatgcttcg gtaatctggg gaacaacctg
     3181 aaaggcatcg cccacaatac cataatctgc tgccttaaaa aaaggagctt cgggatcggt
     3241 gttaaccact acaataacct tggaacggtt aaccccggca aggtgctgaa tagctcctga
     3301 gatacctaca gcaatataca ggttaggcgc aatggcaatt ccggtctgac caacgtgttc
     3361 atggtgagga cgccatccga tatcggcaac aggacgcgag catgccgttg ccgcacccag
     3421 ttcttttgca agttcctcga tgatacccca atgctcagga cccttcatac cacgaccggc
     3481 agaaacaacc atctctgctt ccggaagagc gatttcacct tcgtttttct tcacttcctt
     3541 cacacagaca gcagcagttc cgcaatccgc agcgaaatct tcaacaacgg cagagccacc
     3601 ggcatttgcc aatccgagag aattgggaag aatggagatt accttttttg cagaatgtac
     3661 ttcgataaaa ccgtatgctt tacctgaaaa caaattcact tttaccgaaa atccgcctga
     3721 agtctcagga tagtcgatcg ctcctgctac caatccggca tttaaacgcg cagaaacacg
     3781 cggcgaaaca gcgcgaccaa gcaagtcatg cgaaaagacg ataaggtcgg aagcttcctt
     3841 ttctgcagca gcaagcacca atcgtgtaac ctgttgcgaa tcggcaccgc taagactgcg
     3901 gttgaccaac accttactta caccctgctc accaaaagca gcaagtgcat cattggcagt
     3961 agcaccataa gtgataactg tagcagtagt tcccaattgg gaagcaacct ttgcaccata
     4021 cgacgcagcc tcaagcgcat tcttgttgac ttttccgttg gatgtatctg caaatatcaa
     4081 gactgacata attctctttt tttagtaaat gatcaaataa cttttgcttc gttgtgaagc
     4141 aattcaataa gctgaccggc atgatcagca ggaatcattt tgcaggccga ttttgcagca
     4201 ggcattgcaa atttcttaac agaagtaaga cgatctgctc ctgcaggcgg aaccactgta
     4261 agcggctttg tacgtgcagc catgattcca cgcatattgg gaatacgttg ttcagccatc
     4321 ccttttgcac aggaaacaac aagcggaaga ttcgcctcaa ccacttctgt accaccctga
     4381 acatcgcgct caatggtagc aacattattg ttcacatcca gtttcgaggc aatggaaaca
     4441 taaggaagat caagcagctc tgccaccatt ccgccaacct gtgatccgtt gaaatcgatg
     4501 gtctctttac ctaagagcac catatcgaaa ccttttcctt ttgcatattc tgcaatctga
     4561 cgagcaacga aataggcatc agtagcttct gcatcgatac gtaccgcctc atttgctcca
     4621 atggcaaggg ctttacgaat aacaggctca tcagctgtag ttccaaccgt aatgatggtt
     4681 actgatccgc cggaattttc tgtcagttca agtccacgca ccaaagcgta ccattcatca
     4741 tagggattga caatatattg aacacctgtt tcattgaact tggtgtcgtt atcactaaag
     4801 gcgatctttg ttgtggtgtc gggcgccttg ctgatgcaaa ccagaatttt catgtgtttg
     4861 ttttttgttg gggagcaaaa ataggaatta tcgactttga ggaaacaaaa aaatccagag
     4921 gatgttcatt caaaaaacct tacatttttg acaccaaaga ttcttggttc attttgataa
     4981 tcaaccgcta aggctctgac aggtctctat tgaatttcat tattgttcta attctccttt
     5041 atttcaatcc caatgatttt tcctttttta ctccatttaa agcctgtgaa cctgcctttt
     5101 tctatttttg caggactata aaaattggcc ctatgagaga agtccagttc cgtgaagccc
     5161 tccgtgaagc gatgaatgaa gaaatgcgcc gcgatgagcg tgtgtttttg atgggtgaag
     5221 aagttgccga atacaacggt gcctataaag tgagccaggg gatgctcgat gaatttggtc
     5281 ctaaacgggt gatcgacaca cctattgccg agcttggttt tgcaggtatt ggtgtgggtg
     5341 cagctatgaa tggtttgcgt cctatcgttg aattcatgac atggaacttc gcaattcttg
     5401 ctgccgatca gatcatcaac agtgctgcaa aaatgctgca gatgtcgggc ggacaataca
     5461 atgtccccat cgtttttcgc ggtggtaacg gaactgccgg tcagcttgcc gctacccact
     5521 ctcagagttt cgaagcgttt tatgcgcatg ttccgggatt gaaagtgatt actccttcta
     5581 atccctatga tgcaaaagga ttacttaagg ccgctatccg cgataacgat cccgttgtat
     5641 ttcttgagtc cgaaaaaatg ttcggagata aaggcatgat tcccgaaggt gaatacgttc
     5701 tgcctatcgg agtagccgac atcaaacgca aaggcaagga tgtaaccatc gtttcctttg
     5761 gaaaaatcat gaaagttgcc ttatcggcag ccgaagaatt agcaaaagaa ggcattgaag
     5821 cagaagtaat tgatcttcgc actatccgtc cgatcgacta cgatactgta attgagtccg
     5881 taaaaaaaac caaccgtctg gtggtagtcg aagaaagctg gccgcttgct tctatttctt
     5941 ctgagattgc ctattgggta caacgctatg cattcgatca cctcgatgca ccggtatggc
     6001 gggttaccca atcagacact ccattccctt ttgccaccag cctgatggcg gaagcgcttc
     6061 ccaacaacga acgtgttatt cgcgccgtta aagaagtgtt gtacaaattc tgatcagaac
     6121 taatcttctt tcagccaacc aatcgcctca tcgtcatccc gaaaaacctt
//
LOCUS       DAOMYG010000157         6107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4382_length_6107_cov_9.238764, whole genome shotgun sequence.
ACCESSION   DAOMYG010000157 DAOMYG010000000
VERSION     DAOMYG010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4382_length_6107_cov_9.238764"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1314
                     /gene="kbl"
                     /locus_tag="PK610_06425"
     CDS             124..1314
                     /gene="kbl"
                     /locus_tag="PK610_06425"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HRJ35603.1"
                     /translation="MYGKFQEHLRNELQTIEESGLFKRERIITTAQGASVKVSSGQEV
                     LIFCANNYLGLSSHPRVVEAAHKTLDRHGYGMSSVRFICGTQDIHKELEEKIAGFLGT
                     EDTILYAACFDANGGVFEPLLGEEDAIISDELNHASIIDGVRLCKAQRFRYKNCDMND
                     LEEQLKKAEGCRFKMIVTDGVFSMDGFVAPLDKICDLADKYNALVMVDESHATGFIGK
                     TGRGSIELRNVIGRVDIITGTLGKALGGAMGGFTTGKKEIIEMLRQRSRPYLFSNSLA
                     PHIVGASIAVFDMLSETTELRDKLDANITYFKEGMRKIGLEFKDGESAIVPVMLYDAK
                     LAQEFAARMLKEGIYVIGFFFPVVPKGQARIRVQLSAAHEKEHLDKAIAAFEKVGKEL
                     GVIK"
     gene            1380..1721
                     /locus_tag="PK610_06430"
     CDS             1380..1721
                     /locus_tag="PK610_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35604.1"
                     /translation="MKKDEPATQDPGHYPLLEQYWQDIKDSSHQVLFSNRKWIDLYAG
                     DTVSVNHYELYAESPLKNGIPSPNGKVLWLEESPGNFYEFEILELDSFRLKLRHVEAD
                     RIHFFTKSSRQ"
     gene            1878..2333
                     /gene="rplM"
                     /locus_tag="PK610_06435"
     CDS             1878..2333
                     /gene="rplM"
                     /locus_tag="PK610_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016478129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRJ35605.1"
                     /translation="MDSISYKTIHPNKETVKKEWLLVDATDQPLGRLTSQIAKLLRGK
                     HKPSFSPHVDCGDHVVVINAEKVKFTGNKLSEKQYVRYTGYPGGQRFATPEQVLAKQP
                     GRVVETAIKGMLPKNRLGRAVFHNLHVIAGTEHKYQAQNPKTVNLNDIF"
     gene            2339..2725
                     /gene="rpsI"
                     /locus_tag="PK610_06440"
     CDS             2339..2725
                     /gene="rpsI"
                     /locus_tag="PK610_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018675372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRJ35606.1"
                     /translation="MEVINALGRRKAAVARVYLNSGKGTVTINNTDLKEYFPTAVLQY
                     KINQSFLLTGTEGKYDVTVNVDGGGVNGQAEAIRLGIARALVELDETMKPALKAAGLM
                     TRDPRMVERKKPGQKKARKRYQFSKR"
     gene            2851..3714
                     /gene="rpsB"
                     /locus_tag="PK610_06445"
     CDS             2851..3714
                     /gene="rpsB"
                     /locus_tag="PK610_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRJ35607.1"
                     /translation="MSKVTFNELLDAGVHFGHLKRKWNPNMAPYIFDEKKGIHIIDLN
                     KTIAKLEEASAAIRQIARSGKKVLFVATKKQAKDVVADRVKNVNMPFVTERWPGGMLT
                     NFATIRKSVRKMSNIDKMGADGTLNTISKRERLQVSRQRAKLEKNLGTIADMTRLPAA
                     LFVVDVLKEHIAVAEAKRLNIPVFAMVDTNSDPNQVDFAIPSNDDASKSIAKILDVMI
                     AAITEGLSERKVDKASSGEEDGEEVTDNKRIANFDEEVDAEGKKKAPAKPAAKPAAKP
                     TSAKRPAPRSK"
     gene            3744..4574
                     /gene="tsf"
                     /locus_tag="PK610_06450"
     CDS             3744..4574
                     /gene="tsf"
                     /locus_tag="PK610_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRJ35608.1"
                     /translation="MSSVAISAADVNKLRQQTGAGMMDCKKALIEANGDFEAAVDILR
                     KKGQKVAANRADRSAGEGCVIAAVSADKKFGVVLSLNCETDFVAKNEEFVQLTNDFVK
                     LALDKKSKSKEDFLALDFNGISVNEKLVERTGVIGEKIEIGSFETLEGEFIGHYIHAG
                     NRLATIVAFNKATADDKAKQVAMQVTAMNPISVDEASVPADVVARELEVGKELAIQEG
                     KPAEMAEKIAQGRLNKFYKETTLLNQEFISNPKQTIKQFLAEGDKDLTATAFKRVALS
                     "
     gene            4637..5344
                     /gene="pyrH"
                     /locus_tag="PK610_06455"
     CDS             4637..5344
                     /gene="pyrH"
                     /locus_tag="PK610_06455"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRJ35609.1"
                     /translation="MKYKRILLKLSGEALMGDKQFGIDNQRLNQYAKEIKEIHDKGIQ
                     VALVIGGGNIFRGVQAEQGGMERTQGDYMGMLATMINSMALQAALEQMGVNTRLQSAI
                     KMEQIAEPFIRRRAIRHLEKNRVVIFGAGTGNPFFTTDTAASLRAIEIEADVILKGTR
                     VDGIYTADPEKDPSATKYDRVTFTEVYEKGLNVMDMTAFTLCNENKVPIVVFDMNKSG
                     NLSKVVSGDKVGTLVEF"
     gene            5377..5937
                     /gene="frr"
                     /locus_tag="PK610_06460"
     CDS             5377..5937
                     /gene="frr"
                     /locus_tag="PK610_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRJ35610.1"
                     /translation="MSDVKTIIDLCKEEMNQAISHLENELAKVRAGRANPSMLESVMV
                     DYYGSTVPMSQVANVSTTDARTIRIQPWEKNMLNPIAEAIMNSNLSLNPQNNGEVLII
                     SIPPLTEERRKELCKKAKSEGEDAKISIRNHRRDANEAVKKLQKDGLPEDEAKDGEAK
                     IQDLTNTFSTKIDQLVEQKEKDIMTV"
BASE COUNT         1852 a         1227 c         1437 g         1591 t
ORIGIN      
        1 atcgtgccat ttgggtcttt aaagataatt tctttcacca aaacagcgaa gaaataggga
       61 ttatttggaa ttcctctaaa tttgagcgct aaatcacatt ttcaatcttc caatccaacc
      121 actatgtacg gcaagtttca ggaacatctg cgcaatgaat tacagactat cgaagagtcg
      181 ggtcttttta agcgtgagcg aatcatcaca acagcacagg gcgcctcggt aaaagtgagt
      241 tccggtcagg aagttttgat tttttgtgca aacaattatc tcggactttc ctctcatccg
      301 cgtgtagtgg aagctgcgca taaaaccctt gatcgccacg gatacggtat gagctcagtt
      361 cgtttcattt gcggtacaca ggatattcat aaggaactgg aagaaaaaat tgccggattt
      421 ttaggtacgg aagacacgat tttgtacgcc gcatgtttcg atgccaatgg tggagttttt
      481 gagccattac tgggagaaga agatgcaatc atatctgacg aattgaatca tgcttccatt
      541 attgatggag ttcgtttatg taaagcacag cgtttccgtt acaagaactg cgatatgaat
      601 gatctcgagg agcaattaaa aaaagcggaa ggatgccgtt ttaaaatgat tgttaccgac
      661 ggcgttttct caatggatgg atttgttgct ccattggata aaatatgcga tctggcagat
      721 aaatacaatg cactcgtaat ggtagatgaa tcccatgcaa caggattcat cggtaaaacc
      781 ggacgagggt caattgagtt acgaaacgta attggacgcg tagatatcat tacaggcaca
      841 ctgggtaaag ccctcggagg agcaatgggt ggatttacca ccggaaaaaa agaaatcatt
      901 gaaatgttgc gtcagcgttc gcgaccgtat cttttctcca attcactggc tccgcatatt
      961 gtgggtgcat ctattgcggt tttcgatatg ctgagtgaaa ccaccgaatt gcgcgataag
     1021 ctggatgcca atatcaccta ctttaaggaa ggaatgcgga aaatcggact tgaattcaag
     1081 gatggagaat cggcgattgt tccggtgatg ttgtatgacg ctaaattggc tcaggagttt
     1141 gccgcacgta tgcttaagga aggtatttat gtgatcggtt tctttttccc tgttgttccg
     1201 aaaggtcagg cacgcattcg tgtacaatta tcggctgcac acgaaaagga acacctggat
     1261 aaagccattg ctgcattcga aaaagtaggc aaggaactag gggtgatcaa gtgatgaggt
     1321 tgtttgtcct tttctctttt ctcttttgtt cctgttcgga tgctatgaat actgcagaag
     1381 tgaaaaaaga tgaacctgca acgcaagatc cgggacatta tcccttgctt gaacagtatt
     1441 ggcaggatat caaggacagc tcgcatcagg ttctgttttc aaaccgaaag tggatcgacc
     1501 tgtatgccgg agatactgtt tcggttaatc attacgagct ttatgctgag agtccgctta
     1561 aaaacggaat tccttctcca aacggaaaag tgctttggct ggaggaaagt ccgggtaatt
     1621 tttacgaatt cgagattctt gaactcgatt cttttcgcct gaaactccgt catgtagagg
     1681 ccgacaggat tcacttcttc acaaagagct cgagacaata ggctgtggat cagttagaaa
     1741 caccgtcaat acggcctttg caatattttt ttgcccgcat tcacaggttc gtgttgaaaa
     1801 gtttggctga ttagaattat gttgtaaatt tgcagcccca atttccggga acgaattgat
     1861 ttaaaaaaag aataaaagtg gacagtatca gctacaagac cattcatcct aataaggaga
     1921 cggtcaagaa ggaatggctc ctcgtagacg cgaccgatca gcctcttggt cgcttgacca
     1981 gccagatcgc gaagttgctt cgtggtaagc ataagcccag cttctcacct catgttgatt
     2041 gtggtgatca tgttgtggta attaatgccg aaaaggtgaa gtttacaggt aacaagcttt
     2101 ccgagaagca gtatgtgcgt tatacagggt atcctggcgg acagcgtttt gctactccgg
     2161 agcaggttct tgctaagcaa ccgggtcgag tggtagaaac tgcaattaaa ggtatgcttc
     2221 ccaagaatcg tcttggacgc gctgtgtttc ataacctgca tgttattgcc ggaactgagc
     2281 acaaatacca ggcacaaaat ccaaaaaccg ttaatcttaa cgatattttc taattacaat
     2341 ggaagtcatc aacgcactcg gacgccgtaa ggcagcagtt gcccgcgttt atttaaacag
     2401 cggtaaggga actgtaacca tcaacaatac tgaccttaag gagtatttcc ccacggctgt
     2461 ccttcagtac aaaatcaatc agagtttcct gcttaccggt acagaaggta agtacgatgt
     2521 taccgtaaat gtagacggtg gcggagtgaa tggacaagct gaggctattc gccttggtat
     2581 tgcccgcgca ctagtggaac ttgatgaaac catgaagcct gcgctcaaag cagccggact
     2641 tatgacacgt gacccccgta tggtggaacg taagaagcct ggtcagaaga aagcacgtaa
     2701 gcgctaccag ttctcgaagc gttaattcgc ttttggaatc ggagctcgtt tagcatccaa
     2761 acttgccgga ctcatcgggt gttcccctgg tggctaccgg caggttgctt tataaacaac
     2821 aagtaaaagg aacgtaaaca aaattaaaaa atgtcaaaag ttacattcaa cgaactgctt
     2881 gatgcagggg tgcacttcgg tcacctgaaa agaaagtgga atccaaacat ggcgccttat
     2941 atcttcgacg agaagaaagg tatccacatt atcgatctta acaagaccat cgcaaagctt
     3001 gaagaagcat ctgctgctat cagacagatt gcacgctcag gtaaaaaagt acttttcgtt
     3061 gctaccaaaa agcaagctaa agacgtagtt gcagatcgcg tgaaaaacgt gaacatgcct
     3121 ttcgttactg aacgctggcc cggtggtatg ctcaccaatt ttgcgactat ccgtaaatcg
     3181 gttcgtaaaa tgtcgaacat cgacaaaatg ggagcagatg gtacattgaa tacaatttca
     3241 aagcgtgagc gtctacaggt ttcccgtcag cgcgcgaaac ttgaaaagaa ccttggaaca
     3301 attgcagaca tgacacgtct tcctgctgca ttgtttgttg ttgacgttct taaagaacat
     3361 atcgcagttg cagaagccaa gcgcctgaac attcctgttt ttgcaatggt tgataccaat
     3421 tcagatccta atcaggttga tttcgcgatt ccttcaaacg atgatgcatc aaagtcaatt
     3481 gcaaaaattc ttgatgtaat gatcgctgct attactgaag gtttgagtga gcgtaaggtt
     3541 gacaaagcat catcaggaga agaagatggt gaagaggtga ccgacaacaa gcgtattgca
     3601 aactttgatg aagaagttga tgcagaaggc aagaaaaaag caccggctaa acccgctgct
     3661 aaacctgctg ctaagcctac atctgcaaag cgccctgcgc cacgctcgaa ataatttttt
     3721 aacgatttaa aacactaaac aatatgtcat cagttgcaat ttccgccgca gatgtgaata
     3781 aactacgcca gcaaaccgga gccgggatga tggattgtaa gaaagcactg atcgaggcca
     3841 atggcgattt cgaagctgct gtagatatcc ttcgtaaaaa aggacagaaa gttgccgcaa
     3901 accgcgccga tcgttctgcc ggagaaggat gtgttattgc tgcagtttct gccgataaaa
     3961 aattcggcgt ggttctttcc ttgaattgcg aaaccgattt cgttgcgaaa aacgaagagt
     4021 tcgtgcagct aaccaatgat tttgttaagc ttgcgcttga taaaaaatca aaaagcaaag
     4081 aagattttct tgcattggat tttaacggca tcagcgtaaa cgaaaaactc gttgagcgta
     4141 ccggagttat cggtgagaaa attgagattg gttcattcga aacactcgaa ggagaattta
     4201 tcggtcatta tatccatgcc ggaaatcgtc ttgctacaat tgttgcattc aacaaggcaa
     4261 ctgcggatga taaagcaaaa caagtggcaa tgcaggttac agcaatgaat cctatctctg
     4321 tggatgaagc ttctgttcct gctgatgttg tggcgcgtga acttgaagtt ggtaaagaac
     4381 ttgctattca ggagggaaaa cctgctgaaa tggctgagaa aattgctcag ggccgcctga
     4441 acaaattcta caaagaaacc acactcctta atcaggagtt catcagtaat cccaagcaaa
     4501 ccattaagca attccttgca gaaggagata aagatctcac cgcaacggca ttcaagcgag
     4561 tggcactgag ttaatttatt ccctatgaaa agagagacat tgtctctctt tttttttgaa
     4621 ttgataaacg caaaacatga aatacaaaag gatccttctt aaactcagtg gcgaagcgct
     4681 catgggcgat aagcaattcg gaatcgataa tcagcgtctc aatcaatatg cgaaagagat
     4741 caaggagatt cacgataagg gaattcaggt tgcattggta attggtggtg gaaatatttt
     4801 tcgcggcgtt caggcagagc agggtggaat ggagcgtacg cagggcgatt atatgggaat
     4861 gcttgcaacc atgatcaact ctatggcctt acaggcagcg ctggaacaaa tgggagtaaa
     4921 tacacgttta cagtctgcaa ttaaaatgga acagattgcc gaaccattca ttcgccgccg
     4981 tgctattcgt cacctcgaaa aaaaccgtgt tgtgatcttt ggtgcaggaa caggaaatcc
     5041 cttttttacc accgataccg cagcatcatt gcgtgcaatt gaaatcgaag ctgatgttat
     5101 cctgaaggga acccgtgtag atggaatcta tactgctgat ccggaaaagg atccgtctgc
     5161 tacgaaatat gatcgtgtca cgtttaccga ggtttatgaa aagggactga atgtaatgga
     5221 tatgaccgct tttacgcttt gcaatgagaa caaggttccc attgtagttt ttgatatgaa
     5281 caagtccgga aatttgagca aggttgttag tggagataaa gttgggacct tggtagaatt
     5341 ctgattttct tattttcgca taatacatct atcactatga gcgacgttaa aaccatcatt
     5401 gatctatgta aggaggaaat gaatcaagcc atttctcacc ttgaaaatga acttgccaag
     5461 gtgcgggcag gaagagctaa tccatcgatg ctcgaaagcg ttatggtgga ttattacgga
     5521 tctactgtgc cgatgagtca ggtggcaaac gtaagcacta ccgatgcgcg taccattcgt
     5581 attcagccat gggaaaagaa catgctgaat ccgattgccg aggccatcat gaacagcaat
     5641 ctttcgctga atccgcagaa caacggagaa gtactgatca tttccattcc gccacttacc
     5701 gaagagcgca gaaaagaatt gtgcaaaaag gcaaagtcgg agggagaaga tgccaagatc
     5761 agcattcgta accatcgcag ggatgccaac gaagctgtaa agaaattaca aaaggatggc
     5821 cttcccgaag atgaagcaaa agacggagaa gcaaaaattc aggacctcac caatacgttc
     5881 agtacaaaga tcgatcaact ggtagagcag aaagaaaaag atatcatgac tgtgtaaatt
     5941 caaaaaagcc ccggacatcc ggggcttttt gttcaactaa tttattgttc aggtgtactg
     6001 aatgaaaata aggaatatga accatttttt tgctgtactg tttctgctac tttttaatgt
     6061 gcaaataaac gcagaatcaa atttttcatt ttcaaaggat gggaaga
//
LOCUS       DAOMYG010000158         6092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4400_length_6092_cov_8.152228, whole genome shotgun sequence.
ACCESSION   DAOMYG010000158 DAOMYG010000000
VERSION     DAOMYG010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4400_length_6092_cov_8.152228"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..423)
                     /gene="rplS"
                     /locus_tag="PK610_06465"
     CDS             complement(64..423)
                     /gene="rplS"
                     /locus_tag="PK610_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRJ35611.1"
                     /translation="MDRLATIANELNESKNHSKFKAGDTVTVHYKIKEGNKERIQQYQ
                     GVVIQRRGAGITETFTVRKMSNNIGVERIFPVNSPFIDKVEVNKSGVVRRARVFYLRD
                     LTGKAARIKEKKHAVKA"
     gene            554..2662
                     /locus_tag="PK610_06470"
     CDS             554..2662
                     /locus_tag="PK610_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ35612.1"
                     /translation="MLKTLFSCFFILILVNQSFTQYQSLYREDFTTNSGAWPLYKEGT
                     YTAEVTGGAYAINLKKEGMYWWIWKPVTINPSKPFAIHASFTLKGVSKTGHFGIAWNV
                     KNDKNNYTFAVSREGKHGLFETKEGSYDYKVKWTPSAKKVQMETNHVLKLKSDGSNYT
                     FFLDDVQLCQVPFFKFAFDKSVGFFVENTINVQVDYLEVFQDRGTINAVTGTDLGVVK
                     KENLGANINTAYPEKKPTISHDGKHLYFTRSDDPQNFGDDKSDDIWYAELGNEGLFGK
                     AINLGRPVNNKNNNSCIGVARDNSWIMLKGQYDSNGEYSKQGFSIAHRNGSGWNNPVN
                     IEVKNFYNNNQYQEACINPDGDVLLFAIEREDSYGDKDLYVSKKMSDGSWSEPVNCGG
                     VINSFAAEIGPFIAGDGKTVYFASEGHPGYGDADVFMSVRLDDTWTNWSTPVNLGPTI
                     NTAKWDAYFSTDAKGEWGYMVSTENSIGRTDIFRFKLPQQAKPKPTCVVKGTIINAKT
                     NEPMIGELTFHILETDSMLGKIYATPTDGRFSMILKEGFRYSIDVSYPGYLGERVSVD
                     MSGVTESIEKELVVYLKPIERNTTIELNNVYFEPDKSELLYHSKAQLHWVVLFMEANP
                     NAVILIGGHSSKGNGSEKYYQKLSEKRAKAVYDYLVDLGIPKKRMKYKGFGSSQPVYT
                     SGSAADNGRNRCVNFTIIQN"
     gene            complement(2728..4185)
                     /locus_tag="PK610_06475"
     CDS             complement(2728..4185)
                     /locus_tag="PK610_06475"
                     /inference="COORDINATES: protein motif:HMM:NF021597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HRJ35613.1"
                     /translation="MKRLLLVTLFISSIGILQSKAFCGFYVATADAKLFNKSSQVILV
                     RDGNHTVITMSNDFQGNVKDFAMVVPVPVVLQRNDIRVVNQSLFQSLDAYSGPRLVEY
                     YDENPCYPNYLYDSAMPTLSESVSSTRNESRKKSAEGSGVKIEAEYTVGEYDILILSA
                     IESTGLKTWLTANGYQIPNTAEEVLEPYIKSNMKFFVVKVNLDNKKALGFDNLRPLQI
                     SFDSPKFMLPIRLGMANANGDQDMIVYAFTKTGRVECTNYRTVKIPTDRNVPLPVRQK
                     FGKFYVDLFEKEYKYQGRNCVFLEYAWNVTPSWGGMKCDPCVGPPPLFTDLSQAGVSW
                     SNSGTPVFFTRLHVRYSRSKFPQDLQFQVTPNTEHFQGRYILTNPAYGDLSCDAGQEY
                     LRDLSFRRKREVEELAVLTGWDPSSYYSYITEYNHMIKQNNIRKNELIPVVPVNDHSD
                     GPGGSGKLIFTLLFITVVGSLFILLQRIPVKPVSA"
     gene            complement(4197..5015)
                     /locus_tag="PK610_06480"
     CDS             complement(4197..5015)
                     /locus_tag="PK610_06480"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HRJ35614.1"
                     /translation="MFRLILKEPPVFLRDARNFQILFLSTFLLFGIWNLGWETDILKI
                     GVILATGLAVQSIGIMWSGAPWHSLKSALITCLGLSLLFKANSYSTIALCVFLAIAGK
                     FIIRFNGKHLYNPANFGIVLTILLTGDAWISPGQWGSSAVILFLVGSAGLMVVLRAGR
                     LDTSIAFLGTLFFLEFCRTIVYQGWDYDFLMHKFTNGSLLLFTFFMITDPVTTPNHPI
                     SRLLWASMIAVLTFFLGNWEQIHTAPVWALFFITPFTILFDKLLVAKKFQWINA"
     gene            5135..5653
                     /locus_tag="PK610_06485"
     CDS             5135..5653
                     /locus_tag="PK610_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HRJ35615.1"
                     /translation="MNYKLKLNSITTFIFDFDGVLTDGVVYLLPPKDFIRTMHVRDSY
                     AIQYAVRKGYRIAVITGGNNEMVRERMEYLGVKDVFLKAADKMEVFQSYTSSNNIDPS
                     TILYMGDDLPDFHVMRKVGIPACPNDAAEEIKSIATYVSPHKGGQGCVRDVIEQVLKV
                     HRKWFDKDDLTW"
     gene            5698..>6092
                     /locus_tag="PK610_06490"
     CDS             5698..>6092
                     /locus_tag="PK610_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35616.1"
                     /translation="MLRTIFYIAFTLLALKIQAQQRIVITGTVTEVPENGAKGRPLRD
                     VKVYVENQPKSVVYTNRNGRYILNVEYTDDVRPVIVFHSMLHDLQKREIKKSELRKSG
                     GDTLRIDIVLDFVTLPTVEVSAAPDTVFGS"
BASE COUNT         1790 a         1171 c         1368 g         1763 t
ORIGIN      
        1 caacgttgag caaaaaaaaa gccgtctgtt aggacggctt tctgtatcaa aagaggttga
       61 ggattaagcc ttaaccgcat gtttcttttc tttaatacga gcagctttac cggtaaggtc
      121 acgcaggtaa aatacacgtg cacgacgtac gacacctgat ttgtttactt ccactttatc
      181 aatgaaaggt gagtttacag ggaagatacg ctcaacaccg atgttgttcg acattttgcg
      241 aacagtaaag gtttcagtga tccctgcacc gcgacgctga ataacaacgc cctggtactg
      301 ctggatacgc tccttgttac cttctttaat tttatagtga acagtgacag tgtcaccagc
      361 cttgaattta gagtgattct tgctttcgtt cagttcattt gcgattgttg ccagacgatc
      421 catgacttat cattttttag ggctgcaata gtacgaagat tttttcattt gtcccccata
      481 gagtcgtaaa aaaaaccttt tcgcttgaat tgctgcgaca aaaatatcgt tttttcaggt
      541 aaatttggag gctatgttga aaacactatt ctcctgcttt tttattttaa tacttgtaaa
      601 tcagtcgttt acacaatatc aatcgttgta tcgcgaagat tttaccacca attcaggagc
      661 ctggcctttg tataaggaag gtacatatac cgctgaggtt acaggcggtg cttatgcaat
      721 caacctcaaa aaggagggga tgtattggtg gatatggaag ccggttacca tcaatccctc
      781 caaacccttc gcaattcatg cctcattcac gttgaaaggt gtttcgaaga caggacattt
      841 tggcattgcc tggaatgtga aaaacgacaa gaacaattac acatttgctg tttcgagaga
      901 aggtaaacat ggtttatttg aaacgaaaga aggttcgtac gattacaagg taaaatggac
      961 gccctcagcg aaaaaagtac agatggaaac caatcatgta cttaaactta aaagcgatgg
     1021 aagcaattac acgttttttc tggatgatgt gcagctgtgt caggttccgt ttttcaaatt
     1081 tgcttttgat aaaagtgtag gcttctttgt ggagaatacc atcaacgttc aggtagatta
     1141 tctggaggta tttcaggata gagggacaat aaacgctgta acaggtacgg atctgggtgt
     1201 ggtaaaaaag gaaaacctgg gtgcaaacat caacactgct tatcctgaaa aaaagcctac
     1261 tatttctcac gatggtaaac acctgtattt tacccgcagc gatgatccgc aaaattttgg
     1321 ggacgacaaa agcgatgaca tctggtatgc ggaattaggc aatgaaggtt tatttggtaa
     1381 agccataaac ctcggtaggc cggtgaacaa taaaaacaac aattcctgta ttggggttgc
     1441 acgagataat tcgtggatta tgctcaaggg gcaatacgat tccaatggag aatattccaa
     1501 acaaggattt tcaattgcac atcgcaatgg gagcggttgg aataatcctg taaatattga
     1561 agtgaagaat ttctacaata acaatcagta ccaggaagct tgcatcaatc cggatggcga
     1621 tgttttgctt tttgccattg aacgggaaga ttcctatggc gataaagatt tatatgtttc
     1681 gaaaaaaatg tcggatggtt catggtccga gccggtgaat tgtggtggag taatcaactc
     1741 gtttgcggcg gaaatcggac ccttcattgc cggggatggc aaaaccgttt atttcgcttc
     1801 agaaggacat cccggttatg gagatgccga tgtgtttatg tcggttcgtt tagatgatac
     1861 ttggaccaac tggagcacgc cggttaatct tggtccaacc attaacaccg caaagtggga
     1921 tgcctatttc tctaccgatg ccaaagggga atggggctat atggtttcca ctgaaaattc
     1981 gattggtcgt accgatatat ttcgtttcaa gcttccgcag caagcaaagc caaaacctac
     2041 atgtgtggta aaaggaacca tcatcaatgc caagactaac gaacctatga ttggggaatt
     2101 aacttttcat attctggaaa ccgattccat gctcggaaaa atttatgcaa caccaaccga
     2161 tggacgattt tccatgatcc ttaaagaagg ttttcgttat tctattgatg taagttatcc
     2221 gggttacctg ggggagcgcg tgagtgtgga tatgtcggga gtaacagaat ccatcgaaaa
     2281 agaattggtt gtatacctca aacccatcga acggaatacc actattgaat tgaacaatgt
     2341 gtatttcgaa cccgataaat cggagttgtt gtatcactcg aaagcacaat tgcattgggt
     2401 ggtcttgttt atggaggcca atccaaatgc ggttattctt attggtggac attcctcaaa
     2461 aggaaatgga tctgaaaaat attatcaaaa actttcggaa aagcgggcga aggctgtata
     2521 cgattatctg gttgacttag gtattccaaa aaagagaatg aagtacaaag gatttggttc
     2581 gagccagcct gtttacacca gtggttctgc tgcggataat ggtcgtaacc gttgtgtgaa
     2641 ctttaccata attcagaatt aaggatcata ttaaaaaaca aaaatgctca atgatttcat
     2701 tgagcatttt tatttttagc tagggtatca tgcagaaacc ggtttaaccg gaatgcgttg
     2761 cagcaaaata aacagcgatc ctaccaccgt aataaaaagc agggtaaaga tcagtttacc
     2821 gctaccaccg ggtccgtcag aatgatcgtt aacaggaaca actggaatta gttcgttttt
     2881 acggatgttg ttttgcttga tcatgtgatt gtattcagta atataactgt aatacgaaga
     2941 aggatcccaa ccggtgagta cggcaagttc ttccacttca cgttttctgc ggaacgacag
     3001 atcgcgaagg tattcttgtc ctgcatcaca actcaaatcg ccataagcag gatttgttaa
     3061 gatgtaacgt ccctgaaaat gttcggtatt gggtgtaacc tgaaactgga gatcctgtgg
     3121 gaatttgctg cgactatatc gaacatgcaa tcgggtaaag aaaaccggtg tgccggaatt
     3181 cgaccagctt acaccggctt gtgagaggtc agtaaagagt ggaggagggc caacacaggg
     3241 atcgcatttc attccacccc agcttggagt aacgttccat gcatattcaa gaaaaacaca
     3301 atttcttccc tggtatttgt attccttttc gaaaaggtca acataaaatt tcccgaattt
     3361 ctggcgaacc ggcagaggaa catttctgtc tgttgggatt tttaccgtgc ggtagttggt
     3421 gcattccacg cgacctgttt tggtgaacgc ataaacgatc atatcctgat cgccgttggc
     3481 attggccatt cccaaacgga tgggaagcat aaattttggt gaatcgaaac tgatttgaag
     3541 cggacgaaga ttatcgaatc caagagcttt tttgttatcg agatttacct tcactacaaa
     3601 gaacttcatg ttgcttttga tgtaaggttc caaaacttct tctgctgtat tcggaatctg
     3661 gtatccattt gccgttaacc atgtttttaa tccggttgat tctattgccg acaaaatgag
     3721 aatatcatat tcgcctacag tgtattcggc ttcaattttc actcctgaac cttctgcaga
     3781 tttttttcga ctttcgttac gggttgagct caccgattcc gacaatgtcg gcattgccga
     3841 atcgtacaaa tagttcggat aacaggggtt ttcatcgtaa tactccacca gacgggggcc
     3901 ggaatacgca tcgagcgatt gaaaaagtga ttggttaaca acccgaatat cgttgcgttg
     3961 cagtacaaca ggtaccggta ctaccatcgc aaaatctttt acgtttccct ggaaatcatt
     4021 gctcatggta atgaccgtat gatttccatc gcgaacaaga attacctgtg aggatttatt
     4081 aaagagtttt gcatctgctg tagcgacata aaatccacag aaggctttgc tttgcaatat
     4141 tccgattgag gagatgaaaa gcgttactaa aagaagtcgt ttcatattcg gtaagattaa
     4201 gcgttgatcc actggaattt ttttgctacc agcaatttat cgaagaggat ggtgaaagga
     4261 gtgatgaaga aaagtgccca aacgggagcg gtgtgaattt gttcccagtt accaaggaag
     4321 aaggtgagaa ctgcgatcat ggatgcccat aataagcggg atatcggatg gtttggagta
     4381 gtgaccggat cagtgatcat gaaaaaggtg aacagcagca atgaaccgtt tgtgaatttg
     4441 tgcatcagaa aatcgtaatc ccatccctgg taaacgattg ttctgcaaaa ttcaaggaag
     4501 aacaatgttc ctaagaatgc aatacttgta tcgagtcgtc ctgcgcgcaa taccaccatc
     4561 aatcctgcag atccaaccag aaaaagaatt acagccgaac ttccccactg acccggggag
     4621 atccatgcgt cgccggttaa aagtatggtt agtacaattc cgaagttggc tggattatag
     4681 agatgttttc cattgaaccg aataataaat ttacccgcaa tggcaagaaa tacacaaagt
     4741 gcaatcgtgc tgtaggagtt ggctttgaac aataatgata aacccaaaca ggttattaat
     4801 gcacttttca gcgaatgcca gggtgcgccc gaccacatga tgccgatcga ctgtactgcc
     4861 aatccggttg ctagtatcac tcctattttt aggatatccg tttcccatcc taaattccat
     4921 ataccgaaca gtaagaatgt agaaagaaaa aggatctgaa agtttctcgc atcgcgcaga
     4981 aaaaccggtg gttcttttag aatcagtcga aacatagtga tcagttttga tgctgtacaa
     5041 cctttttgtg ttttggttca ttttttttcg aaatgccttg ttttcaagta atttggtttt
     5101 agtgcatgtc aaattctaat tgtattttca cgctatgaat tacaaattga aattgaattc
     5161 catcaccact ttcatttttg attttgacgg ggtgctgaca gatggcgtgg tttatctcct
     5221 gccaccaaaa gattttataa gaaccatgca tgttcgcgat tcctatgcga ttcaatatgc
     5281 cgttcgtaaa gggtatagaa ttgcggtaat aaccggaggc aataatgaaa tggtaaggga
     5341 acgcatggaa tacctcggag taaaggatgt ctttctaaaa gcagcagata aaatggaggt
     5401 ttttcaatct tatacttctt ccaataatat cgatccttca accattttgt atatgggcga
     5461 tgatcttcct gattttcatg taatgcgtaa agtcggaatt ccggcttgtc cgaacgatgc
     5521 tgctgaggaa attaaatcga ttgccactta tgtttctccc cacaagggcg gacagggctg
     5581 tgtgcgcgat gtgattgaac aggttctaaa ggtgcacaga aaatggtttg ataaggatga
     5641 tctgacctgg tagatttttt tttatggaat cggaatgctt tttgcatctg aattgaaatg
     5701 ttgcggacca tcttctatat cgctttcact ttactggcgc tgaaaatcca ggctcagcaa
     5761 cgtattgtta tcaccggtac agttactgaa gtgcctgaaa atggtgcaaa aggacgtccc
     5821 ttgcgtgatg taaaagttta tgtagaaaat caaccaaaat cggtagtgta tacaaatcgt
     5881 aatggaaggt atatcctaaa tgtggaatac acagatgatg tgcggcctgt gattgttttc
     5941 cattcaatgc tgcacgattt acagaagcgg gagatcaaaa aaagtgaact gcgtaagtca
     6001 ggtggcgata cactgcggat tgatatagta ctcgattttg ttacccttcc taccgttgaa
     6061 gtatccgctg cacctgatac cgtatttggt tc
//
LOCUS       DAOMYG010000159         6090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4403_length_6090_cov_8.806959, whole genome shotgun sequence.
ACCESSION   DAOMYG010000159 DAOMYG010000000
VERSION     DAOMYG010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4403_length_6090_cov_8.806959"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..1416)
                     /locus_tag="PK610_06495"
     CDS             complement(73..1416)
                     /locus_tag="PK610_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRJ35617.1"
                     /translation="MMMAKRKNQVKIFVLDTSVILHDHNSINNFDDNFVAIPIVVLEE
                     LDNFKRGHDSKNFEAREFIRIMDKLSGNQSLQDWIPLEGNNKGKFKIVMTGKHGGTDA
                     NVVYGERKADHRILNAALSLMDEEKDKQVVLVSKDINLRLKAKALNILAEDYETGKIS
                     DLSNFNTGRIAIENVDPDEIRKIFTDGFTENTSILNGDKVNNQFYILKSDKSSTLAYY
                     NPFVERVERVEKQFTYGIKPRNAEQTFALHAIMNDDVKLVTIQGVAGTGKTLLALAGA
                     IEQRKKYNQIILARPIVPLSNRDIGYLPGDANEKINPYMEPLWDNLKFIKSQFSENEK
                     RYKAIDEMVQEQKIVVTPLAFIRGRSFSNIFFIIDEAQNLTPHEVKTIITRAGENTKI
                     IFTGDIFQIDTPYLDEQSNGLSYIIDRLKGSKLYAHVTLEKGERSELANLANDLL"
     gene            1660..2358
                     /locus_tag="PK610_06500"
     CDS             1660..2358
                     /locus_tag="PK610_06500"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HRJ35618.1"
                     /translation="MKRLILLSLPLFLFSCRPWRYDVGPVITEDRNLAPFTALSVYGE
                     PDIVFTLDTGIVVRVEAPEKLQDDIHTSVSNGRLRVEQTGWREWFNGKTKVYIAQTGF
                     DDLYFSGSGKISGAPLIMNNGIIKHFGSGDLDLTISSPKLFIQLFGSGDLTLQGQIPS
                     LELSTTGSGDINAEPVYAVKAIVNCTGSGDIRVRVSDTLQVNISGSGDVRYWGNPSVL
                     NVNITGSGDLIHMN"
     gene            2384..3031
                     /locus_tag="PK610_06505"
     CDS             2384..3031
                     /locus_tag="PK610_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35619.1"
                     /translation="MKTLLLIVAALSLQIAQANSSDSLRKRFTNDDETLLGKGRPLGF
                     FVAWQNKPMLLNEQAAWMTGGQISLVFGRKLNVGFAGYGLVSPVKSDRLDANGYRMYF
                     DMGYGGLMLEPVLFTRKMIHLTIPVLLGGGGVGFRQGYDPYDPQWEDIDWDNYNGDAF
                     FIAEPGLNLELNLFKWMRFHAGASYRFATDGYFGSSLSGQLSGWSANFGLKLGWF"
     gene            3219..3746
                     /locus_tag="PK610_06510"
     CDS             3219..3746
                     /locus_tag="PK610_06510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRJ35620.1"
                     /translation="MQDCKRNDRSAQEELYRLYSGAMYNVSYRILQNREEAEDVLQES
                     FLDAFTSIQTFRGEASFGSWLKRIVVNRSINETKKRKVHFGELKSDVAEEPEESQHDL
                     PDCTIEDVKTAMKKLPEGFRIVFSLYMFEDWSHRDIAEKLGITESTSKSQLNRAKKKM
                     AELIVEQTSKGHGKG"
     gene            3712..4281
                     /locus_tag="PK610_06515"
     CDS             3712..4281
                     /locus_tag="PK610_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35621.1"
                     /translation="MNKQAKDMEKDNWKSFLDENRDAFEHDRPSERVWGNIRAQLPEA
                     APAMVKLSTVLKVAAVAVLVVSISFWVLLDRKSESVAKQVVHDSENLSSSGLVLAQLS
                     PEYREMEVFYVSRIQSAMSEVKDLPEDEELLRSLRELDTEFGNLREEMKENVNEAEII
                     EAMIMTYKMKLDLLERYLDSWKEIEDDKN"
     gene            4305..5411
                     /locus_tag="PK610_06520"
     CDS             4305..5411
                     /locus_tag="PK610_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35622.1"
                     /translation="MNKILDKTVLLCILMLPLCGFAGTEHKILPFTKAKSIEKSFPLK
                     AQTQVEVVSKYGDIKFRNWDKDSVRFEVNVIAESDQEDYAEMLLGLAEIKFIAAGGSV
                     RSELVWGEQLNAVKRNSMEMMLNLKKNHRIRIDHVVYLPKSTSVLIENRFGDVEMETI
                     TGKLIAKVHHGDFRAGLVSEARLLNVKYGRIETEEITVGEIQLSFGSMNLLAATELRI
                     QSSGSEIRIGNAGQLHVKSSNDKFDVEKLHSLSGNSSLTSFRIRELLYAIDFDIRFGD
                     IVVRKISRDYDHINLQSGSSDIELIFDPAASFGFDLDLDNVRSLVINSVSKKINNDSQ
                     IEKRRIISGSFGKQTDSPVKVKLRTKAGNVRMEQ"
     gene            5466..>6090
                     /locus_tag="PK610_06525"
     CDS             5466..>6090
                     /locus_tag="PK610_06525"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HRJ35623.1"
                     /translation="MRRIIFSLLCVFVFLPELEAQIVNIENQRLRNPKNGWSGHLDLS
                     FNVTKNTRMIYQIGNRNRIYWKKDQHLVSFLTDFLYVNAGRQNYVNYGFQHARYSYNS
                     KQFKRIFLEGFEQLQYNQIQLIRFRSLLGGGARAVVLDRDSASVSLGAFVMGEYEEEA
                     TELVNRQIRYSTFFSFDFQFNKVTGINSITYYQPDFISPDDYRVSTEV"
BASE COUNT         1669 a         1150 c         1438 g         1833 t
ORIGIN      
        1 ggcaaaagcg ctcagcactg atgtatccac cgagttactg aatacatttt ttggaatagg
       61 aaatccgttg ttttacaaca gatcatttgc aaggttagcc aactcagaac gttctccttt
      121 ttccagggtg acgtgtgcat agagcttgct tcctttcagt cggtcgatga tgtaggataa
      181 tccgttcgac tgttcgtcga ggtacggtgt atcgatctgg aaaatatctc ctgtaaaaat
      241 gatcttggtg ttttctccgg cgcgggtaat gatggttttt acttcgtgcg gagtcaggtt
      301 ttgtgcttca tcaataataa agaaaatatt ggagaaactt cttccgcgga taaatgcgag
      361 tggtgtaaca acaattttct gttcctgcac catttcatcg attgccttgt agcgcttttc
      421 attttcggag aactgactct taataaattt cagattgtcc caaagcggtt ccatataagg
      481 gttgatcttc tcattggcat ctccgggcaa gtatccgata tcgcggttgc tgagtggtac
      541 gatgggacgc gcaagaatga tctgattgta tttttttcgt tgctcaatag ctcctgcaag
      601 cgcaagcaat gtttttccag ttcctgctac accttgtatg gtaactagtt tcacatcatc
      661 gttcatgatg gcgtgcagtg caaaggtttg ctctgcatta cggggtttga taccgtatgt
      721 gaattgtttc tccacacgtt ctacacgctc cacaaaggga ttgtaataag caagagtaga
      781 ggacttgtcg ctttttagaa tgtagaattg attattcacc ttgtcgccat tcagaatgga
      841 ggtgttttcg gtaaagccgt ctgtgaatat tttacgaatt tcatcgggat caacattttc
      901 aatcgcaatt ctgccggtat tgaaattact cagatcactg atctttcctg tttcatagtc
      961 ttcggcaaga atattcagcg ctttggcttt taaacggaga ttgatgtctt ttgaaacaag
     1021 aacaacttgc ttgtcttttt cttcatccat taaactcaag gcagcattca gaatacggtg
     1081 atcggctttt cgttcgccat acaccacatt cgcatccgtg ccgccgtgtt ttcccgtcat
     1141 cactatcttg aactttcctt tattgtttcc ttcaagcgga atccaatcct gcagactctg
     1201 attgccggat agtttatcca tgatgcggat gaattccctt gcttcgaaat ttttggagtc
     1261 gtgtccccgc ttaaaattgt cgagctcttc gaggactaca atcgggatgg ctacaaaatt
     1321 atcatcaaaa ttattgatgg aattgtggtc gtgaagaatc acggaggtgt ccaggacgaa
     1381 aatcttcacc tggttcttgc gcttggccat catcatgctg taaaggttta gaacgttgtt
     1441 agtgcaattt aacaggcttt actaaccgaa gagagagaag gagccaaaag aaatgaagag
     1501 ggaaatgtgg attactgtcg ataaacagct ttccttaccg gtgttcatta tttgtgtcat
     1561 cctagtgaaa agccttgatt ttattcggtt tttaaaaaat gtttgaaaat cctgtgcaac
     1621 ctttttcaac ttctttgtcc ttgaagtaaa cacaaaaaaa tgaaacgtct tatcctgctt
     1681 tcacttcctc tttttctttt ttcctgccgg ccctggcgtt atgatgttgg tccggtaatt
     1741 accgaagacc gcaatctggc tccgtttaca gcactttctg tttatggtga accggatatt
     1801 gtttttacgc tggataccgg aatcgtggtt cgtgtggaag caccggaaaa attacaggac
     1861 gacatccata cttctgtaag caatggtcgc cttcgggtgg aacaaactgg ttggcgggag
     1921 tggtttaacg gaaagacaaa agtttatatc gcgcaaacag gttttgatga tctttatttt
     1981 agcggatcag gaaaaatttc cggagcacct ttgatcatga ataatggtat catcaaacat
     2041 ttcggtagtg gtgatttgga tttaacgatt agttcaccaa aactttttat tcagcttttc
     2101 ggttcagggg acctcacact acaaggccaa attccatcgc ttgaactcag caccacaggc
     2161 agtggagaca tcaatgcaga accggtttac gcagttaaag caattgtcaa ttgtaccggt
     2221 tctggtgata tccgtgtacg cgtttcggat accttgcagg taaacattag cggcagcggc
     2281 gacgtgcgct attggggaaa tccttcggta ttgaatgtta acattaccgg ttccggtgat
     2341 ttgatccaca tgaattaatt gtaaaacgaa aatgcatatg aaattgaaaa cactacttct
     2401 tattgttgct gcgctttctt tgcagattgc acaagccaat tcgtccgata gtttacgtaa
     2461 acgatttacc aacgacgatg aaactttact ggggaaaggc cgtccactcg gattttttgt
     2521 tgcatggcag aataaaccaa tgttactcaa tgagcaggca gcatggatga caggcggaca
     2581 aatctcgctc gtgtttggtc gtaaactgaa tgtgggattt gcaggatacg gattggtttc
     2641 acctgtaaaa agtgatcgtt tggatgccaa tggataccgc atgtattttg atatgggata
     2701 tggcggtctg atgctggagc cggtgttgtt tacacgaaag atgatacacc ttacaattcc
     2761 tgtactgctt ggtggagggg gagttggttt tcgccagggg tacgatcctt acgatccgca
     2821 atgggaagat attgattggg acaattacaa cggtgatgca ttttttattg cagaacccgg
     2881 attaaatctc gaattgaatt tgttcaaatg gatgcgtttt catgccggag cttcttaccg
     2941 ttttgcaacg gatggctatt ttggttcatc attaagcggc caactttccg gctggagcgc
     3001 taatttcgga ttgaagttgg gttggttttg atgaagcaga aacctcctat cttcggttta
     3061 cgctttatca ttggcgagga agcggaaata agcgaatttg gacttgctga aggcaaaagc
     3121 tggggtacgt ggtcgcaatt agtaatttag cacattcaaa gccaagataa aaagcaattc
     3181 aggatttggt aactacacca cacatacacg ataagcttgt gcaagactgt aagcgcaacg
     3241 atcgctcagc tcaggaggag ttgtatcgat tgtacagcgg ggcaatgtac aatgtgagtt
     3301 accgcatatt gcaaaacaga gaagaagcag aagatgtttt gcaggagtcg tttcttgacg
     3361 cattcacttc cattcaaact ttccggggag aagcgtcgtt tggatcatgg ttaaagcgaa
     3421 ttgtggtgaa tcgttcgatc aatgaaacga aaaaacgaaa agtacatttt ggtgaattaa
     3481 aatcggatgt ggcagaagaa ccggaagaga gtcagcacga tttacccgat tgtacaattg
     3541 aggatgtaaa aacagctatg aaaaaattgc ctgaggggtt tagaatcgtt ttttcattgt
     3601 acatgtttga agattggtcg caccgtgata ttgcggagaa attggggata accgaatcga
     3661 cgtccaagtc gcagttgaac cgtgcgaaga aaaaaatggc agaattaatt gttgaacaaa
     3721 caagcaaagg acatggaaaa ggataactgg aaatcgtttc tcgacgaaaa ccgtgatgcg
     3781 tttgaacacg atcgtccttc tgaaagagta tgggggaata ttcgcgcgca acttccggaa
     3841 gctgcaccgg ctatggttaa gttgagcaca gttcttaaag tagctgcagt ggctgtactt
     3901 gtggtgagta tctctttttg ggtattgctc gatcgtaaaa gcgaatctgt ggcgaagcag
     3961 gtggttcatg attcggagaa tttgtcttct tccggattgg ttcttgcgca gctttcacca
     4021 gagtatcgcg aaatggaagt attttatgta agtcgtattc aatcggcaat gagtgaagtg
     4081 aaggatttac cggaagacga agagttgctt cgttctttgc gtgaactcga taccgaattt
     4141 ggaaacctcc gtgaagaaat gaaagagaac gtgaatgagg cggaaattat tgaggcgatg
     4201 atcatgacct ataaaatgaa actggattta ctggaacggt atctcgattc ctggaaagaa
     4261 attgaagacg ataaaaactg atcatacaca ctaactgagg gtatatgaat aaaattttag
     4321 ataaaacggt gttgctgtgt atcctgatgc tgccgctttg cggatttgca ggcacagaac
     4381 acaagatact tccgtttaca aaagcgaaaa gtattgagaa gtcttttccc ttaaaggcgc
     4441 aaacacaggt tgaagtcgtt tcgaaatatg gcgatattaa attccgtaac tgggataagg
     4501 atagtgtacg ttttgaggta aatgtgattg ccgaaagcga tcaggaagat tatgcagaaa
     4561 tgcttctagg acttgcagaa attaaattca ttgctgcagg tggttctgtg cgctcagaat
     4621 tggtgtgggg cgaacaatta aatgccgtta aacgcaattc gatggaaatg atgctcaatc
     4681 tgaaaaaaaa tcatcgcata cgtatcgatc atgttgtgta tttgcccaag tcgacatctg
     4741 ttttaatcga aaatcgtttt ggggatgttg aaatggaaac cattaccggt aaattgatag
     4801 ctaaagttca tcatggcgat tttcgtgcgg gattggtttc ggaagcacgt ttactcaatg
     4861 tgaaatacgg acgcatcgag actgaggaaa ttacggttgg cgaaattcag ctttcattcg
     4921 gctccatgaa tttgcttgcc gcaactgaac ttcgtataca atcatcgggt tcagaaatcc
     4981 ggatcggtaa tgcaggacaa ctccatgtga aatcttcgaa cgataaattc gatgtcgaaa
     5041 agctgcacag tctaagcggg aattcttcgc tgacatcttt tcgtatccgc gaattgcttt
     5101 atgctatcga ttttgatatt cgttttggag atattgtggt tcgtaagatt tcacgcgatt
     5161 acgatcacat caatctgcag tcgggttcga gtgatattga actcattttt gatccggcag
     5221 cttcctttgg tttcgatctg gatctggata atgttcgtag tctggtgatc aattctgtga
     5281 gcaagaaaat caataacgat agtcagatcg aaaagcgaag gatcatatcc ggtagtttcg
     5341 gaaagcagac cgattcaccc gttaaggtga agttgcgaac gaaagcagga aacgtgcgta
     5401 tggagcaata aacaattttc gggatttgtt ttttgtatgg ggctaaacag taattttgcc
     5461 cacccatgcg ccggattatt ttttctttat tgtgtgtgtt tgtgtttctt cccgaattgg
     5521 aagcgcagat tgtcaatatt gaaaatcaac gtctccgcaa tcccaaaaac ggttggagcg
     5581 gacatcttga tctttccttt aacgtcacca agaacacccg aatgatatat cagatcggta
     5641 atcgcaaccg gatatattgg aaaaaggatc agcatttggt ttctttttta accgattttc
     5701 tttacgtgaa tgcaggtcgg caaaactatg tcaattacgg atttcagcat gcgcgttatt
     5761 cttacaattc caaacagttc aaacgcattt ttctggaggg ctttgagcag ttacaataca
     5821 atcagatcca gttaatccgt tttcgttcac tcctgggcgg aggcgcccgt gcggtggtgc
     5881 ttgatcggga ttcggcctct gttagtttgg gggcttttgt aatgggcgag tacgaagaag
     5941 aagcaacaga attggttaac cgtcagattc gatacagtac gtttttctcc ttcgattttc
     6001 aattcaataa ggttacaggc atcaattcca taacgtatta ccagcccgat tttatctcac
     6061 ccgatgatta cagggtgtcg actgaagttt
//
LOCUS       DAOMYG010000160         6090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4404_length_6090_cov_8.096437, whole genome shotgun sequence.
ACCESSION   DAOMYG010000160 DAOMYG010000000
VERSION     DAOMYG010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4404_length_6090_cov_8.096437"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..2935)
                     /gene="carB"
                     /locus_tag="PK610_06530"
     CDS             complement(119..2935)
                     /gene="carB"
                     /locus_tag="PK610_06530"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRJ35624.1"
                     /translation="MPKDPSIKSVLIIGSGPIIIGQACEFDYSGSQAARSLREEGIEV
                     TLINSNPATIMTDKVVADNVYLKPLEPESIEYILQHHKIDAVLPTMGGQTGLNLCIKC
                     EEQGIWEKYNVKLIGVDIKAIEITEDREKFRQLMNQIGIGQAPSFIAKSFLEGKEIAQ
                     KIGFPLVIRPSFTLGGTGASFVHKAEDFDRLLTRGLHASPIHEVLIDKAVIGWKEFEL
                     ELLRDKNDNVAIICSIENFDPMGIHTGDSITVAPAMTLSDSTYQKMRTMAIKMMRSIG
                     NFAGGCNVQFAVSDDEKEDIIAIEINPRVSRSSALASKATGYPIAKIASKLAIGYQLD
                     ELNNQITKTTSAYFEPTLDYVIVKIPRWNFDKFQGAERELGLQMKSVGEVMGIGRSFQ
                     EALQKACQSLEIRRNGLGADGKELTNQEQILQSLEHPSWNRLFHIYDAIKLGIPFKTI
                     KEKTKIDEWFLRQIEELVMMEKEIEKHTIQNIPYELLFEAKQKGYADRQLGHLLRCLE
                     SEVFQKRQDQNIRRVYKLVDTCAAEFPAQTPYYYSTFESENESVVSNKKKIVVLGSGP
                     NRIGQGIEFDYSCVHGVLAAKECGYETIMINCNPETVSTDFDTADKLYFEPVFWEHIY
                     EIIQHEKPEGVIVQLGGQTALKLAEKLHRYGIKIIGTSFEALDLAEDRGSFSSLLKDN
                     NIPYPKFGVVESAEQAIELSKDLGFPLLVRPSYVLGGQKMKIVINEQELETHVVDILR
                     DMPENKILLDHFLSGAIEAEADAICDGENVHIIGVMQHIEPAGIHSGDSYAVLPPYNL
                     GDLVMQQIEDHTRKIAVALRTVGLINIQFAIKDEIVYIIEANPRASRTVPFIAKAYDE
                     PYVNYATKVMLGVNKVTDFKFNPRKEGYAIKIPVFSFEKFPNVNKELGPEMKSTGEAI
                     RFIDDLKDPYFRQIYAERNLYLSR"
     gene            3074..3898
                     /locus_tag="PK610_06535"
     CDS             3074..3898
                     /locus_tag="PK610_06535"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ35625.1"
                     /translation="MESRSVESNGMSLEYFSFGNGPQVLLCFHGFGRRAEDFFYFREE
                     LEKNFTVYSFNVFHHGKSEYPGDRIERNTLRPDELKALISLFLDQHSIERFSLMGYSM
                     GGKITLMLLHLFAERINHMYLLAPDGIVYNQWYRFTSKNRIGNSIYKSILRKPGFFFF
                     ILRLLRKTRLISDKFMRFVMANLDTRNKRELVYKVWMTLRNIDPSPSESSKIIRKLNL
                     PVYLFYGKHDRIIKSSSGKSFASKAGDSVRFIEVECGHNLFTPDTKSCLKKVFSIE"
     gene            complement(3888..4370)
                     /locus_tag="PK610_06540"
     CDS             complement(3888..4370)
                     /locus_tag="PK610_06540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ35626.1"
                     /translation="MHNDPGKTEGYLDFISPVLNALNEPISGKNKLLDYGAGPHAFLG
                     GHLEKFGWNVSVYDPFFYPDKIVLENNYDFILLVEVMEHFHRPAAEFDHLFSKLKPGG
                     KIVCMTNMHAEKINFDTWYYKNDPTHVFFYHQKAIEFLAEKHKCSNLKTEGRLIVFTQ
                     "
     gene            4694..>6090
                     /locus_tag="PK610_06545"
     CDS             4694..>6090
                     /locus_tag="PK610_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35627.1"
                     /translation="MKNIDFKKWLPYLAAIVLFAGLTLSYFKPITDGYELRQADVINY
                     KGMAKEIQDFRKIYGQEPLWTNSMFGGMPAYQIDVQNSSNILLKIDRVLQLGFPGVIG
                     YFFLMFIGFYILMLCMNVDPWLAIVGAVAYAFSSYFMIIVEAGHNTKVHALAYAPAVL
                     GGFLLLMRGRMWLGAAVFTFFLALQLLSNHVQITYYLFLLLFIIGIGEFIKLIKEQGV
                     ILNFAMRTGLIGLAGTLAVMASSSNLWNTYEYGKDTTRGASELTISADGKMGSADQGL
                     DKDYITGWSYGIDESWTFVFPSAKGAGSGYILSDKDFQDSKKVKPQVKAFIQEKAMES
                     QQSQGRAGFMVNRYWGDQPFTSGPTYMGALVVFLFVLSFVFVTDPLKWAVLIASVLFL
                     MLGWGRNLMGFTEFFIENFPGYNKFRSVSMIISMIELVMPFMAILFVKEVITKREEMK
                     AKTRKFLIASGITGG"
BASE COUNT         1609 a         1246 c         1352 g         1883 t
ORIGIN      
        1 tttttattgt cgggtacctt ttcctctgtt cggttggatg cagcacgtcg gaaaactccc
       61 atcagccaca taatggctac ggcaataatg aggatctccc aaatattgac cttacccatt
      121 aacgactgag atacaagttg cgttcagcat agatctgacg gaaataggga tctttcaaat
      181 catcaataaa acgaatggct tctccagtcg atttcatttc tggtccaagc tccttgttca
      241 cattcgggaa tttctcgaag gagaaaacag gaattttgat cgcataacct tctttgcgcg
      301 gattgaattt gaaatcggtg accttgttaa ctcccaacat caccttagtt gcgtaattca
      361 cataaggctc atcgtatgct tttgcaataa agggaacagt acgcgatgca cgtggattgg
      421 cttcgatgat gtatacgatt tcatctttta ttgcaaactg aatattgatc aatcctacag
      481 tgcgaagcgc aacggcgatc ttgcgtgtat gatcttcaat ttgctgcatc accagatctc
      541 ccaggttgta gggtggaagt actgcatacg aatctcctga gtgtataccc gctggttcaa
      601 tatgttgcat tacgccaatg atgtgaacgt tctcgccgtc gcaaatggcg tcggcttcgg
      661 cttctattgc tcctgaaaga aaatgatcaa gcaagatctt gttttcgggc atgtcgcgca
      721 gaatatctac cacgtgggtt tcaagctcct gctcgttgat tacaattttc attttttgtc
      781 cgccaagaac ataactcgga cgaacaagca gtgggaaacc taaatctttt gataattcga
      841 tggcttgttc tgcactctcg accactccga atttcggata gggaatgttg ttgtctttga
      901 gcaaactcga aaaactacca cggtcttctg caagatcaag cgcctcgaag cttgtgccga
      961 taatttttat tccgtaacga tgtagctttt cggcaagttt caaggcagtc tgaccaccaa
     1021 gctgcacgat tactccttca ggtttttcat gctgaatgat ctcgtagata tgctcccaga
     1081 ataccggttc aaaatagagt ttatctgctg tgtcaaaatc ggtcgatact gtttcggggt
     1141 tgcaattgat catgatggtt tcgtaaccgc attcttttgc agcgagtacg ccatgcacac
     1201 aactgtagtc gaactcgatt ccctgaccaa ttcggtttgg accggagccc agcaccacaa
     1261 tttttttctt gttgctaact accgattcat tttcgctttc gaaagtcgaa tagtagtaag
     1321 gtgtttgtgc aggaaattcc gctgcacagg tatcaactaa cttgtaaacc ctgcggatgt
     1381 tctggtcttg ccgtttctgg aaaacttcac tttccagaca acgcaacaaa tgacccagct
     1441 gacgatcagc atatcctttt tgtttggctt caaacaagag ttcgtaaggg atattctgaa
     1501 tggtgtgttt ttcgatttct ttttccatca tcaccagttc ttcaatctgg cgaaggaacc
     1561 actcgtcgat tttggttttt tccttgatgg ttttaaaagg gatgccaagt ttaatggcat
     1621 cgtaaatatg aaacagtcga ttccagctgg gatgctccag gctttgcaga atttgttcct
     1681 ggttggtgag ctctttacca tcggcaccaa gtccgttgcg acgtatctcg agcgactgac
     1741 acgctttttg taaggcttcc tgaaaagaac ggccgatccc catcacttct ccaaccgatt
     1801 tcatttgcag ccccaattcg cgttctgcac cttgaaactt gtcgaaattc caacggggta
     1861 tttttacaat aacataatcg agtgtaggtt caaaatacgc cgaagtagtt ttggtgatct
     1921 gattattcag ttcatccagc tgatatccga tagccagttt agaagcaatt tttgcaatgg
     1981 gataccctgt tgcctttgat gcaagtgcag aggatcgcga aacgcgtggg ttgatctcaa
     2041 tggcgatgat gtcttctttt tcatcgtcgc tcactgcaaa ctgaacatta catcctcctg
     2101 caaaatttcc aatggagcgc atcattttaa tggccattgt acgcatcttt tggtaggtgc
     2161 tgtcggatag tgtcattgcc ggtgcaaccg taatggaatc gccggtgtga attcccatcg
     2221 gatcgaaatt ctcgatggaa caaatgatgg ctacgttgtc gtttttatcc cggagtaatt
     2281 caagctcaaa ttctttccag ccaattactg ctttatcaat gagtacttca tgaataggcg
     2341 aagcatgcag accacgtgta agcaagcggt caaaatcttc ggctttgtgc acaaatgatg
     2401 ctcctgttcc acccagtgta aaactcggac gaatcaccag cgggaaaccg atcttttgtg
     2461 ctatttcttt tccttccaga aaggattttg caatgaaaga aggtgcctga ccaataccga
     2521 tttggttcat gagttgacga aacttctcac gatcctctgt tatctcgatg gctttgatat
     2581 caacgccaat cagtttcaca ttgtactttt cccagattcc ctgttcctca cattttatgc
     2641 aaaggttcag tcccgtctga ccacccatcg taggcaaaac agcatcaatt ttatggtgct
     2701 gaaggatgta ttcgatcgat tccggttcca gaggcttgag gtacacattg tcggccacta
     2761 ctttatccgt cataatggtt gccggattgg aattgataag cgtgacttct atgccttctt
     2821 cgcgtaatga acgggctgcc tgtgagccgg aatagtcaaa ttcgcaggcc tgtccgatga
     2881 tgatgggtcc cgatccgatg atcagaactg atttaatgct ggggtccttg ggcatagagc
     2941 aatgaactga ggttgaaaat cgggcaaagg taagcgctga gagggacttg gcattgtgga
     3001 aaactgtgcg atttaggggg atttttgaac aaaaacttca attttcaggc tcaagagaat
     3061 gtaacttagc aaaatggaaa gcagaagtgt agaaagcaac ggaatgagtc ttgagtattt
     3121 ttcctttggt aatggacctc aggttttgtt gtgcttccac ggatttggac gtagggcaga
     3181 ggattttttc tatttcaggg aagagctcga aaaaaacttt actgtctatt cctttaacgt
     3241 gtttcaccac ggaaaatccg aatatccggg tgatcggatc gaaaggaata ccttgcgtcc
     3301 ggatgaactt aaagcactga tcagtctttt tttagatcaa cattccattg agcgtttttc
     3361 tctgatgggt tacagcatgg gagggaaaat taccttgatg ctattgcatt tgtttgctga
     3421 aagaataaac cacatgtatt tgttggctcc cgatggtatt gtgtacaatc agtggtaccg
     3481 atttaccagc aaaaacagaa ttggaaacag tatctataaa tcaatcttac gtaaacccgg
     3541 ttttttcttt tttattctcc ggttactacg gaaaacccgt ttgattagcg ataaattcat
     3601 gcggtttgta atggctaatc tggatacccg caacaagcga gaattggtct ataaagtttg
     3661 gatgaccctg cgcaatatcg atccttctcc atccgaaagt tcaaaaatta ttcggaaact
     3721 aaatcttccg gtttatcttt tttatgggaa acatgatcgt attattaaaa gcagcagtgg
     3781 aaaatccttt gccagtaaag caggcgattc tgtgcgattt atagaggtgg aatgcggtca
     3841 caatctgttt acacccgaca ccaaaagttg cttgaaaaaa gtattttcta ttgagtaaaa
     3901 acaatcagtc ttccctctgt tttcagattg ctgcatttgt gtttttctgc cagaaattca
     3961 attgcttttt ggtgataaaa aaatacatga gtgggatcgt ttttgtaata ccatgtatcg
     4021 aaatttattt tttcagcatg catgttcgtc atacaaacga tttttcctcc gggttttagt
     4081 ttggaaaata agtgatcaaa ttccgcagcc ggccggtgaa aatgttccat gacttccaca
     4141 agtaaaatga aatcgtaatt attctcaagg acgattttat cgggataaaa aaagggatca
     4201 tacaccgata cattccaacc gaatttttct agatgtccgc ccaggaatgc atgaggcccg
     4261 gcaccgtaat ccaatagctt atttttcccg gaaattggtt catttaatgc gttcagaacc
     4321 ggactaataa aatccagata cccttctgtt tttccgggat cattatgcaa ttcatacctg
     4381 gttttttccc tctcaggttc ggggaaatgt tcatgatgaa ggaatatact tttgcagaag
     4441 gtacatcgga ggtataatct gtcactatga atggaaaaag cctcggattt tgccttgcaa
     4501 agggggcaat gggaagaaaa tgaagaagtt aaattttcct tctgatgcat tgattaatag
     4561 cctttttact gcttattgtt tgggaaggca gctgatttta gttgttaaag atattgaaaa
     4621 caaggcgact gaggtttaat tgagcgcgtc tcattcgcag cttttattac atttgatttc
     4681 tccaaattat ccaatgaaaa acatcgattt caaaaagtgg cttccttatc tggcagcaat
     4741 agtattgttc gcagggctta cgctctccta tttcaaacca attaccgatg gttatgagtt
     4801 aaggcaggcc gacgttatca actataaggg aatggcaaag gaaattcagg atttccggaa
     4861 gatttatggt caggagcccc tttggaccaa cagcatgttt gggggtatgc ctgcttacca
     4921 gatcgatgtt cagaatagtt ccaatattct cctcaaaatt gacagagtac ttcaattggg
     4981 tttcccggga gttattggat atttcttcct gatgtttatc gggttttaca tcctgatgct
     5041 ctgtatgaat gtcgatccct ggctggccat tgtcggtgcc gttgcttatg catttagttc
     5101 gtatttcatg atcattgttg aagcggggca caatacgaaa gtccatgcac ttgcatatgc
     5161 acctgccgtt ctcggtggtt ttctgctgct tatgcgggga cgtatgtggc tgggagctgc
     5221 cgtatttaca tttttcctcg cgcttcaatt gctttctaat cacgtacaga taacctacta
     5281 tttgttctta ttgcttttca taatcggtat tggagaattt attaagctga ttaaagaaca
     5341 gggggtgatc ctgaattttg caatgcgaac cggtttgatc ggactggcag gaacactcgc
     5401 cgtaatggca tcatcgagta atttgtggaa tacctacgaa tatggtaaag acactacacg
     5461 cggagcatct gaactcacta tcagtgctga tggaaaaatg ggttctgcag atcagggtct
     5521 cgataaagat tatattaccg gatggagtta cggaatcgat gagtcgtgga cgttcgtttt
     5581 cccgagcgcg aaaggagccg gatctggtta tattctttcc gacaaagatt ttcaggattc
     5641 caaaaaagtg aaacctcagg ttaaagcctt tatccaggaa aaagcaatgg aaagtcagca
     5701 atcgcaggga cgtgccggat ttatggtcaa tcgttattgg ggagatcagc cgtttacttc
     5761 tggtcccaca tacatgggcg cattggttgt ttttctgttt gtactgagtt ttgtgtttgt
     5821 tactgatccg cttaaatggg cggtattgat cgcatcggtg ttattcctta tgctgggatg
     5881 gggtagaaac ctgatgggat tcacggaatt cttcattgaa aattttccgg ggtacaacaa
     5941 attccgatcg gtttctatga tcatttccat gatcgaattg gtgatgccct ttatggccat
     6001 tctatttgtg aaggaagtaa ttacgaagcg ggaagaaatg aaagccaaaa ccaggaagtt
     6061 tttgattgca tccggaataa ccggtggatt
//
LOCUS       DAOMYG010000161         6081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4413_length_6081_cov_7.691503, whole genome shotgun sequence.
ACCESSION   DAOMYG010000161 DAOMYG010000000
VERSION     DAOMYG010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4413_length_6081_cov_7.691503"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="PK610_06550"
     CDS             complement(<1..868)
                     /locus_tag="PK610_06550"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ35628.1"
                     /translation="MSDTITKGDKKLIRSWAFYDWANSAYPLVITTAIFPIFYENVTR
                     SDVKDELGNIIGHSDTVTFWGHHFHNTELYSYVVGLSFILVSLFSPILSGVADYTGRK
                     KLFMRIFCYLGAVSCASLYFFNKDYLGLSLLSVFFASIGFWGSLVFYNAYLPEIAEPE
                     DHDSISALGFGLGYLGSSILLIINLVLIQAFEMNARWAFVTVAIWWIGFAHISFARLP
                     KGQRNGEPRKNILTNGFHELGNVWKELKHHKQLKRFLASFFIYSMGVQTVMLMAVLFA
                     KKEIEGLEESSLI"
     gene            complement(929..2083)
                     /locus_tag="PK610_06555"
     CDS             complement(929..2083)
                     /locus_tag="PK610_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ35629.1"
                     /translation="MKLRILLSAVLLSSLVVNAQDDKNLVKNPSFEAINGKLKKPKQL
                     ELATNWTSPTAEKADLYSRSVKELPVSAPENTNGKETPADGNNYAGVMMYSFGDKAPR
                     TYIMTELLGPLKAGVKYCVRFDVSLSDNSKYAVNNIGAHVSKKPFGFEDKRSIMAETH
                     VKNSKNKIYNNTYGWETVCAVFTASGGEKYLTLGNFSPTKDTKNEKVTKPKDSKAQQL
                     PFAYYYIDKVSVFQLDSIEECQCEAKEKIEEATVIVGEGYTSNKEFKLKEKIAHEKVF
                     FTHLSTDINQIGLESINVLVDLMNNNPDAVIEVHAHSESSEVAAATKVDTAKDLAKRR
                     GEAVVKQLEKAGISTNRIVLVIDDDKDPVSKGESESEKARNRRVTFQLRE"
     gene            complement(2080..3117)
                     /gene="hemE"
                     /locus_tag="PK610_06560"
     CDS             complement(2080..3117)
                     /gene="hemE"
                     /locus_tag="PK610_06560"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HRJ35630.1"
                     /translation="MQLKNDLILRAARGEQTERYPVWLMRQAGRILPEYRAVRSKLRD
                     FKELVETPERAAEVTIQPVDILDVDAAIIFSDILVVPEAMGFTYQMLEAKGPWFEHPL
                     NGPDDLKRLNVPDVHDQLHYVMEALKITKKELNGRVPLIGFAGAPFTILAYIIEGQGS
                     KTFSKARKLLYTQPDLCHTALDRITRTTIAYLKAQIAAGADMVQLFDSWAGFPTPEQY
                     REFGSKYIRQICEAITEVPVTVFAKGAYFAREEFAKIPCRTIGLDWNMDMRESRAIVG
                     PEKTLQGNLDPCVLYGSPEVIRKETIKMLESFDGGLHIANLGHGVYPDTDPEHVKVFI
                     DTVKAYQKPRS"
     gene            complement(3136..3486)
                     /locus_tag="PK610_06565"
     CDS             complement(3136..3486)
                     /locus_tag="PK610_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35631.1"
                     /translation="MKTFFLSLFCFLLFSFSVAAQTQGEVVILRVEYAKKSSGIEHRM
                     QLDAGISTQHSLSGKVINEKGVVRIKTDEKTESFTNEVDLLSYLQKNGFRLLQVFNQT
                     IMGETYLVYLLERP"
     gene            complement(3532..4149)
                     /locus_tag="PK610_06570"
     CDS             complement(3532..4149)
                     /locus_tag="PK610_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35632.1"
                     /translation="MKKCITLLQALALTMMLFIGSTQKSNAQAMEQGNVGVNVYYGFP
                     NLASYLIRSFADEGADAKLTSIGPIGGNFEYMASDKVGVGLEVNYSSFGIEWDDADSA
                     SGVVYNYKASVNRTRIMPRFNIHFGASENFTGYFGIAAGYLMTKYKLTTNDPNRQNES
                     SPGLLPFAMRIAFGGRYYFSDNIGIHMELGLGGGALIHAGLSFKF"
     gene            4265..4858
                     /locus_tag="PK610_06575"
     CDS             4265..4858
                     /locus_tag="PK610_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35633.1"
                     /translation="MKTLKLSVITLIAGSFLFTSCGGGVSEELKKELGAFETEWTKAG
                     EAATAFGASISEEDSAMAQMRPSELPDSLKMNATPEQLAVIDSLNTVCSGHTEKVAEI
                     KSTFDGFKASWDKDSKDWADWKAKVEKGEVKEEEAKTELENWKKKLAAANTSIGEWQS
                     ALDGVSSECSATCKAQKDAIAAIPAAPVKEEKGKKKK"
     gene            complement(4911..6008)
                     /locus_tag="PK610_06580"
     CDS             complement(4911..6008)
                     /locus_tag="PK610_06580"
                     /inference="COORDINATES: protein motif:HMM:NF025749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4421 family protein"
                     /protein_id="HRJ35634.1"
                     /translation="MFYHKKIIVVIALVLLAGTSFCQVDSLPSKKRKSSYLDTNYVVR
                     YKDRLVVSLYQSTRNYKFDMSPIGLKDSSGADLIKYSTNSNLGTGIGFDYDKISFSIG
                     RSTPGTEKEIRQKGKTSSFNLAFSLNGKRHRIETFYRSYMGFYDSNYGKFGYQANDTN
                     IIHYQNPRMSVKEFRTKFFWYFNKKRRFSYAAAYSNTQRQLKSAGTFLVAANVYGLVV
                     KSDTGLIPYPSRPFYPESPDLIRVRSIGFSFKPGFSYNLVLFKRLFANVTFLWGPELQ
                     FRKFSDTDRGDKKSAAFGFSEAEFRGSIGYNGKYLYFYYFLMSDLNRISTKTIFIDKT
                     LIYQGGVIGYRFKFNNKFSKWLQANKYYNMI"
BASE COUNT         1721 a         1288 c         1301 g         1771 t
ORIGIN      
        1 taatcagact gctttcttcg agaccttcaa tttctttttt tgcaaacaaa acagccatca
       61 gcattacggt ctgtactccc atgctgtaaa taaaaaaaga agccagaaag cgtttgagtt
      121 gtttgtggtg ttttaattct ttccaaacat ttcccaattc gtgaaatcca ttggtaagaa
      181 tatttttacg cggttctccg ttgcgttgtc ctttgggtaa tcgggcaaaa ctgatgtgcg
      241 caaaacctat ccaccaaatg gccacagtca caaaagccca acgcgcattc atttcgaagg
      301 cctgaatcaa taccagattt atgataagca aaattgaact tcccaggtac cctagtccaa
      361 acccgagagc actaatgctg tcgtgatctt cgggttcggc tatttcgggg agataggcat
      421 tgtaaaatac cagtgaaccc cagaatccaa tggatgcaaa aaagaccgac aataaactta
      481 gtcccagata atctttattg aaaaaataaa gtgatgcaca ggaaactgct cccaggtaac
      541 aaaatatacg catgaacagt ttcttcctgc cggtataatc tgctacacct gatagtattg
      601 gtgagaagag ggaaaccaaa ataaacgaca atcccactac atacgaatac aattccgtgt
      661 tgtggaaatg atgtccccaa aacgtaacag tatccgaatg cccaattatg tttccgagtt
      721 catccttaac atccgaacgg gttacgtttt cgtaaaaaat cggaaaaatg gctgtagtaa
      781 ttacgagcgg ataagcagag ttagcccaat catagaatgc ccatgatcga atcagttttt
      841 tatcgccttt ggtaatggtg tccgacatcg aagcgaagat aaaaaaaagg gaggtccaat
      901 gacctccctt taaatatcga tactatgatt attctcttaa ctggaatgta acacgtcggt
      961 ttctggcttt ttctgattcc gattctccct tggaaacagg gtctttgtca tcatcaatca
     1021 ccagaacaat tcgattggtt gaaattcctg ctttttcaag ttgtttcacc acagcctcac
     1081 ccctgcgttt ggccaggtcc ttggctgtgt ccaccttggt tgctgctgca acttctgagc
     1141 tctcagaatg cgcatgcacc tcaataacag cgtcagggtt gttgttcatg aggtcaacga
     1201 gtacgtttat cgactcaaga ccaatctgat tgatgtctgt gctcaaatgc gtgaagaata
     1261 ctttctcatg cgcaattttt tccttgagct tgaactcttt gttggaggta tatccctcac
     1321 caacaattac agtcgcttct tcaatcttct cttttgcttc gcactgacat tcttctatgc
     1381 tgtcgagctg gaaaaccgat actttgtcga tataataata cgcgaaagga agctgttggg
     1441 ctttagagtc ctttggcttg gtcacttttt catttttggt atcctttgtt ggactaaagt
     1501 ttccaagtgt caggtatttt tcaccaccac ttgcagtaaa aactgcacaa acggtttccc
     1561 atccataagt gttgttgtaa attttattct tcgaattttt tacatgcgtt tctgccataa
     1621 tagagcgctt gtcttcaaat ccaaaaggct ttttcgaaac gtgcgcacca atgttattta
     1681 cggcatactt cgagttgtcg gaaagactta catcgaatcg tacacaatat tttacaccgg
     1741 cttttaatgg gcctaataat tcggtcataa tgtaagtacg gggcgcttta tcgccgaatg
     1801 aatacatcat aacaccggca taattgtttc cgtctgcagg tgtttctttt ccattggtgt
     1861 tttcgggtgc acttaccggt aattctttaa ccgagcggga atacaaatcc gctttttcag
     1921 ctgtaggtga agtccagttt gttgccaatt caagctgttt tggcttttta agctttccgt
     1981 taatagcttc aaagctgggg ttttttacca ggtttttatc gtcctgtgcg tttaccacca
     2041 gactgctcaa cagcactgcg ctcaatagga ttcttagttt catgatcgtg gtttttggta
     2101 tgcttttact gtgtctatga atactttaac atgttcagga tcggtgtcgg ggtaaacacc
     2161 gtggcctaaa ttagcaatat gcagtcctcc gtcaaaactt tccagcattt ttatggtttc
     2221 cttccgaata acttcaggac taccatataa aacacatgga tcgagatttc cctgcaaggt
     2281 cttttccggt ccaacaatag cacggctttc gcgcatatcc atgttccagt cgagaccaat
     2341 tgtccgacag gggatttttg caaattcttc acgtgcaaaa taggctcctt ttgcaaaaac
     2401 ggttacgggt acttcagtga tcgcttcaca gatctgtctg atgtatttgc ttccgaattc
     2461 gcggtattgt tccggtgtgg gaaatccagc ccaggaatcg aacagttgca ccatgtctgc
     2521 cccagctgca atttgtgctt tcaggtaggc gatggtggtg cgcgttatcc ggtcgagggc
     2581 agtatgacaa agatccggtt gagtgtataa caacttccgt gccttgctga atgttttgct
     2641 tccctgacct tctataatgt aggccagaat agtaaaagga gcacctgcaa aaccgatgag
     2701 cggaactctg ccattcagtt cttttttggt gattttcagc gcttccataa cataatggag
     2761 ctggtcgtgt acatcgggaa cattcaatcg tttaaggtcg tccggtccgt ttaaaggatg
     2821 ttcgaaccat ggccctttcg cttcgagcat ttggtaagta aagcccattg cttcgggtac
     2881 taccaaaata tcagagaaaa tgattgctgc atcaacgtca aggatatcaa ccggttgaat
     2941 ggtaacctct gcggctcgtt caggtgtttc caccaattct ttaaaatcac ggagtttgga
     3001 gcgtacagcc cggtattcag gaaggattct tccggcctga cgcatcagcc aaacggggta
     3061 acgttctgtt tgttcacccc ttgccgcacg gagaatgagg tcgtttttta gttgcatgcg
     3121 ggcaaaaata cacaatcagg gcctctcaag gagatatacc agataagttt cgcccattat
     3181 tgtctggtta aaaacctgaa gcaagcgaaa tccatttttc tgcagatagc ttaaaaggtc
     3241 aacttcgttg gtaaaggatt ccgttttttc gtcggtcttt atacgaacaa cgcctttttc
     3301 gttgatcact ttaccgctta gggaatgttg ggttgaaata cctgcatcca attgcatccg
     3361 gtgttcgatg ccggaagatt ttttagcata ttccacgcgc aaaatcacaa cctcaccctg
     3421 agtttgtgca gctacggaaa atgaaaaaag caggaagcag aaaagtgaaa ggaaaaaagt
     3481 tttcatggtt ggaaagaaaa aaggtccccg tttttggtgg ggaccttatg attaaaactt
     3541 aaagctcaat ccggcgtgga taagtgctcc accaccaagt ccgagttcca tatggatacc
     3601 aatattgtcg gagaaataat agcgaccacc aaaagcaata cgcattgcga aaggcaataa
     3661 tcctggactg gattcattct gacggtttgg atcgttagtg gttaatttgt atttagtcat
     3721 taaataaccg gccgcaatac cgaaatagcc cgtgaaattc tcgctagcac caaaatggat
     3781 attgaagcgg ggcatgatgc gggtacggtt aacgctggct ttatagttat aaactacacc
     3841 ggaagcagag tcagcatcgt cccactcgat cccgaatgag gaataattta cttcgagccc
     3901 aacaccaact ttatccgatg ccatgtattc aaagtttccg ccaattggtc caatcgaggt
     3961 tagcttggca tctgcacctt catccgcaaa gcttctgatc aggtaggacg ccaggttagg
     4021 gaaaccgtag taaacattta caccgacatt cccttgttcc atggcctgag cattggattt
     4081 ttgggtgctg ccgatgaaca gcatcatggt cagggcaagt gcctgcaaca gagtaataca
     4141 ttttttcatt ttaatgggtt ttgattaatc tgattaaaat tagaaaaaaa aatgaagtgc
     4201 acaaaccgca acttcgattt cgaattaaac ttgcagatat atttgcaaaa aaaaactcaa
     4261 aaccatgaaa acactaaaac ttagcgtaat tactcttatt gcaggatcct tccttttcac
     4321 ttcttgtgga ggtggcgttt ctgaagaatt aaagaaggaa ctcggtgcat tcgaaacgga
     4381 atggacaaaa gcaggggaag ccgcaactgc ttttggagct tctatctctg aagaagattc
     4441 agccatggct caaatgcgtc cttctgaact gcctgattcc ttaaaaatga atgctactcc
     4501 tgagcagctg gctgttattg attcactgaa tacggtttgc agtggtcata cagaaaaagt
     4561 tgctgaaatc aagtcgacct ttgatggctt caaagcttca tgggataagg atagcaagga
     4621 ttgggcagat tggaaagcaa aagttgaaaa aggtgaggtt aaggaagaag aagcaaaaac
     4681 tgagctcgaa aactggaaaa agaaactcgc tgctgcaaat accagtatcg gtgaatggca
     4741 aagcgcttta gatggcgttt catcagagtg ttctgctacc tgcaaagcgc agaaagatgc
     4801 aattgcagct attcccgcag cgccagttaa agaagaaaaa ggcaaaaaga aaaagtaagc
     4861 aagtattcat aaacagaaag cccgaaccta caggttcggg ctttctgttt ttagatcatg
     4921 ttgtaatatt tattggcctg aagccacttg ctgaatttgt tattgaattt aaaccggtat
     4981 cctatcactc ctccctgata gatgagtgtt ttatcaatga aaattgtttt agtagatatt
     5041 ctgttcaggt cgctcatcag gaaataatag aaatataggt acttcccgtt gtacccgata
     5101 cttcctctga attctgcttc ggaaaatcca aatgccgcac tttttttatc gcctcggtcg
     5161 gtatcgctga atttcctgaa ctgcaattcc ggtccccata agaaagttac gttggcaaac
     5221 aagcgtttaa acagtacaag attatagctg aatcccggct taaaactgaa tccgatggaa
     5281 cgcacccgta tgaggtcagg actctccggg taaaaggggc gggaggggta gggaatcaat
     5341 cctgtatctg actttaccac caatccgtaa acattcgccg cgacaaggaa tgtacctgcc
     5401 gatttgagct gacgttgtgt attggaatag gcggctgcat acgagaacct gcgcttttta
     5461 ttgaaatacc agaaaaattt tgtcctgaat tccttaacgg acattctcgg gttttgataa
     5521 tgtatgatat tggtgtcgtt ggcctggtat ccgaatttac cgtagttgct gtcgtaaaaa
     5581 cccatgtaac tgcggtaaaa ggtttcaata cggtgacgct ttccatttaa tgaaaaagcc
     5641 agattaaacg atgaggtttt acctttctgt cggatctcct tttccgttcc aggggtactg
     5701 cgaccaatac tgaacgatat tttatcgtaa tcgaatccga ttccggtacc cagattggag
     5761 ttggtgctgt acttgatcag gtctgcgccg gagctgtcct ttagacctat aggtgacata
     5821 tcaaacttat agttcctggt gctttggtaa agagaaacaa ccagacgatc cttatagcga
     5881 accacatagt tggtgtctaa ataggaggat ttcctttttt ttgagggaag ggaatcaacc
     5941 tgacaaaaac tagtgccggc taatagcacc aatgcaataa caacaattat ctttttatga
     6001 taaaacatga ttaaagctag gcaatttttg aaatccagaa aggtcaattt ggcttaacaa
     6061 ctaattaaac ttgataggaa t
//
LOCUS       DAOMYG010000162         6073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4419_length_6073_cov_10.845630, whole genome shotgun sequence.
ACCESSION   DAOMYG010000162 DAOMYG010000000
VERSION     DAOMYG010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4419_length_6073_cov_10.845630"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PK610_06585"
     CDS             complement(<1..689)
                     /locus_tag="PK610_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35635.1"
                     /translation="MIRYIFALAFSFIFCSGFSQNENDALRYSMIQFGGTARFVGMAG
                     SMGALGADASCASTNPAGYGRFTRSEFSLGLNYLDAGSKVSFNGTESQAGRGNFNVSQ
                     FALIGAFKPSYSEDWKQIQFGVSYERYALFHNRISINGENNSSLLDAFVGEANGVAEQ
                     YLLADRPFYSGPAYWAYLIDPLDTIANTYISAIPDGRVEQTRTIDRKGSIGDIHCLLG
                     MPPEAFGGIAIA"
     gene            complement(703..2004)
                     /locus_tag="PK610_06590"
     CDS             complement(703..2004)
                     /locus_tag="PK610_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35636.1"
                     /translation="MIAAVAGFSLTAMSWMSCGLSGKSDLYADHDDVYTQRGRYVQGG
                     TVATTSNTKSTIDTTEQDYYDPSYSGATAATNTRSTYDEDDYYYSNRMRRSGSCGNYG
                     YYSPHYYTSPCYSCGGYGYNPYGNGYNNWGNNSWGYNPYGYNNWGYNNYNNWGYGSYG
                     YNSWGNGYAYNPYGYGYNPYGYGYNPYGYGYNPYGYSSYGNSYGGWGGSEMGSNNYYG
                     HRNGIGTGSTSSGSYSARPRTSEGVESTSPELANGYQTKSAERNSVYSEAQKGSFVSR
                     SATTSTNNGTVVQSRPVDRTNMMPSSSNTVSRTQSSGSATNYNSSNSGVSRSTNSGNT
                     TNSSAWSGTRSGGSNSGTSTSSGVSRSGSTSSGSSGTPTRTTRTESSGSSGSYQSAPT
                     RSTGGSGSSGGGSRSSGSSSGGSSGGGGSRSSGGGGTRGPR"
     gene            2127..3602
                     /gene="proS"
                     /locus_tag="PK610_06595"
     CDS             2127..3602
                     /gene="proS"
                     /locus_tag="PK610_06595"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRJ35637.1"
                     /translation="MSKAITSKEKNYSDWYNDLVVEADMAEHSDVRGCMVIKPHGYAI
                     WEKMQATLDKMFKDTGHVNAYFPLFIPKSYLSKEAAHVEGFAKECAVVTHYRLKNAED
                     GSGVVVDPEAKLEEELIVRPTSETIIWNTYKGWIQSYRDLPLLINQWANVVRWEMRTR
                     LFLRTAEFLWQEGHTAHATREEAIEEAEKMLNVYAEFAEHFLAMPVIKGIKTASERFA
                     GAEETYCIEALMQDGKALQAGTSHFLGQNFAKAFDVKFANKEGKLDFVWATSWGVSTR
                     LMGALIMTHSDDKGLVLPPRLAPLQVVIVPIYKGEEELAKISERALEFKSALQKKGIS
                     VKYDDSDKQRSGWKFAEYELKGVPVRVAIGPRDFQNGTVEIARRDTLTKEVVSHEGLA
                     DRIEKMLEEIQQSLFDKAKSRLVASTRKADSYDAFKKMLDEDGGFILAHWDGTPETEE
                     RIKEETKATIRCIPLDQEKEDGKCMVSGKPSNGRVVFARAY"
     gene            3610..4305
                     /locus_tag="PK610_06600"
     CDS             3610..4305
                     /locus_tag="PK610_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35638.1"
                     /translation="MENTSPSANDLILKSLKEKSSVKQDVFYLSKARFSEFKKILAEF
                     ANLVSKEASKIDERLKVVFTDKGEYEAQLQVAGDVLVFHLHTNVFLFDSDHSLWKTSY
                     LKDDASRGYCGIIHVYNFLSDSFKYNRLGDLGYLVARVFVNRENHFMVQGKRQLGFLY
                     NDFINSQFSPEQMKAVVESIVLYTIDFDLFTPPYDLIKEVSLMEIKELSDTLQMKTGK
                     RLGFKFQADSDDF"
     gene            4386..4457
                     /locus_tag="PK610_06605"
     tRNA            4386..4457
                     /locus_tag="PK610_06605"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4419..4421,aa:Glu,seq:ttc)
     gene            4503..4757
                     /gene="rpsT"
                     /locus_tag="PK610_06610"
     CDS             4503..4757
                     /gene="rpsT"
                     /locus_tag="PK610_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRJ35639.1"
                     /translation="MANHKSAIKRIRSNDAKRVRNRYYHRTTRNAVKAIRTSNNKKEA
                     LGLVSAVFSMLDKLAKRNVIHKNKAANIKSSIHHHVNALA"
     gene            4888..5583
                     /gene="radC"
                     /locus_tag="PK610_06615"
     CDS             4888..5583
                     /gene="radC"
                     /locus_tag="PK610_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRJ35640.1"
                     /translation="MEERKNTIRQWAEDDRPREKLIRLGRRALSDAELLAILISSGNE
                     RETALDLSRRLLSEAAGNSLNRLARLSVGDLQKFRGIGEAKAITILAALELSVRRRAA
                     EKEIRPTISGSKDAFQYIEPLLSDLSHEEFYVIYLNRANVVISHARVSSGGITGTVVD
                     ARMVYKGVLDAQATSIILCHNHPSGNLRPSNEDISLTKKLKEGGKLLDINVLDHLIVC
                     NNAFYSFADDGLI"
     gene            5628..>6073
                     /locus_tag="PK610_06620"
     CDS             5628..>6073
                     /locus_tag="PK610_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010134067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HRJ35641.1"
                     /translation="MKKILTIVGARPQIIKAAALSRVIRNQFSDVLQEKILHTGQHYD
                     DSMSAVFFRELGIPQPDFQLSVGSGSHGQQTAKMLEGIEKVLLDEAIDALVVYGDTNS
                     TVAGALAASKLRIPVVHIEAGLRSFNKSMPEEINRILTDHVSSLLFT"
BASE COUNT         1726 a         1269 c         1408 g         1670 t
ORIGIN      
        1 gcgatggcga tgccgccgaa agcctctggc ggcatgccga gcaggcagtg tatgtcacca
       61 atggaccctt ttcgatctat agttcttgtt tgttctacac gtccgtcagg aattgcagaa
      121 atgtaggtgt ttgcaatagt atcgagcgga tcgataagat aggcccaata agcaggacca
      181 gagtagaagg gacgatctgc taacaggtat tgttcagcaa ctccgttcgc ttctcctaca
      241 aatgcatcca gtaatgaact gttgttttct ccgtttatcg aaattctatt gtgaaacaag
      301 gcataccgtt catacgacac accaaattga atctgtttcc agtcttcgga gtagctgggt
      361 ttaaacgcgc ctatcaaagc aaattgggaa acattaaagt tccctctgcc tgcttgcgat
      421 tctgtaccgt taaaactgac tttagagccg gcatccagat agtttaatcc caatgaaaat
      481 tccgatcgtg taaatcgtcc ataaccggcc ggattcgttg aggcgcagga agcatctgca
      541 ccgagagcac ccattgaacc ggccattcct acgaaccggg cggtacctcc aaactgaatc
      601 atggaatacc gcaatgcatc attttcattc tgggagaaac ccgaacaaaa aatgaaagaa
      661 aaagcaagtg caaagatgta acgtatcact attcaaacga tgttaacgtg gaccacgagt
      721 tcccccgcca ccgctgctgc gactaccgcc acctcccgaa gagcctcccg aagaagatcc
      781 tgagctgcgg cttccgccgc cgctacttcc ggatccgcct gtagagcggg ttggtgcaga
      841 ttggtaagat cctgaagacc ctgaagattc tgtgcgtgtt gtgcgcgttg gtgttccgga
      901 tgaacctgaa gaagttgaac ctgaacgtga aactccactt gaggttgaag tgccggagtt
      961 gcttccacct gaacgtgttc cgctccaggc agatgaattg gtagtgttac ctgagttggt
     1021 agaacgacta actcctgagt tggatgaatt gtaattagtt gcagaaccag atgactgtgt
     1081 tctactcact gtattactcg aagaaggcat catgttcgtg cgatcaaccg gtcgcgactg
     1141 caccaccgtt ccattgttgg ttgaggtggt agcagaacgg ctgacaaaag atcctttttg
     1201 agcttctgaa taaacagaat tgcgctccgc gcttttggtt tggtagccgt ttgcaagttc
     1261 aggacttgtt gattctactc cctcactagt acgtggtctt gcggaataag atccggaaga
     1321 agtagagcct gttccgattc catttcggtg tccataataa ttattggagc ccatctctga
     1381 acctccccag cctccatagc tgttcccata tgagctatat ccataggggt tatatccata
     1441 tccgtaaggg ttataaccgt atccataagg attgtagcca tatccataag ggttgtacgc
     1501 gtagccatta ccccaactgt tgtagccata agagccgtat ccccagttat tgtagttgtt
     1561 gtatccccaa ttgttatatc cgtaaggatt gtacccccag ctattgttgc cccagttatt
     1621 gtacccgttt ccatagggat tgtatccgta tccaccgcag ctgtaacagg gagatgtgta
     1681 gtaatgcgga ctgtaataac cgtagtttcc acaagagccg gatctgcgca ttcggttgga
     1741 ataatagtaa tcgtcctcgt cgtatgtgct tctggtgtta gttgctgcag tagcccctga
     1801 ataggaaggg tcataataat cctgctctgt agtatcgata gtgctttttg tgtttgaggt
     1861 tgttgccaca gttccaccct gcacatatct gcctctctgg gtgtacacat catcgtgatc
     1921 ggcatacaga tctgatttcc ctgacaatcc gcaggacatc caactcatag ccgtcagcga
     1981 aaaccctgca accgcagcga tcatcctgaa atttgaattc tttgttgcca taggaaccat
     2041 cctttctgtt aaaacgtgtg gaacgattaa ttgttacttt tgcaaagtac aaaaaatgta
     2101 ccattttaac gtcacagaat tgtcagatga gcaaggcgat tacctcaaaa gaaaagaatt
     2161 actccgattg gtacaatgac ctcgtagtag aagcagatat ggccgagcat tcggatgtac
     2221 gcggatgtat ggttataaag cctcacggat atgctatttg ggaaaaaatg caggccaccc
     2281 ttgataaaat gttcaaggat acaggacacg tgaatgccta ttttccgctt tttataccga
     2341 agagttatct aagtaaagaa gcagcgcatg ttgaaggttt tgccaaggaa tgtgcagttg
     2401 ttacccatta cagacttaaa aacgctgaag atggaagtgg tgttgttgtt gatccggaag
     2461 ccaaattgga agaagagctt attgtacgcc ctacttccga aaccattatt tggaatacat
     2521 acaaagggtg gattcaatcg taccgcgact tgcccctgct tattaaccaa tgggcaaatg
     2581 tggttcgctg ggaaatgcgt acacgtttgt ttttgcgcac cgctgagttt ctctggcaag
     2641 aaggccacac agcgcatgct actagagaag aagcaatcga agaagctgaa aaaatgttaa
     2701 atgtgtatgc tgaatttgcc gaacattttt tagcaatgcc agtgataaag ggaattaaaa
     2761 cagcatccga acgatttgca ggtgcagaag aaacgtattg catcgaggca ttaatgcaag
     2821 atggcaaggc tcttcaagcc gggacatccc attttcttgg acagaatttt gccaaagcgt
     2881 ttgatgtgaa atttgccaac aaagaaggga agcttgattt tgtttgggca acatcatggg
     2941 gtgtttcaac acgattaatg ggagcattaa tcatgacaca ttcagatgac aaaggactcg
     3001 tattacctcc tcgattggct cctttacagg ttgttattgt accgatttat aagggcgaag
     3061 aagaacttgc aaaaatttct gaaagagcat tggaatttaa atccgcactt cagaaaaaag
     3121 gtatcagtgt aaaatacgat gattccgaca agcaacgttc aggttggaaa tttgcagagt
     3181 acgaactgaa aggagtgcct gttcgcgttg caattggtcc acgagatttc caaaatggaa
     3241 ctgtggaaat agctcgtcgt gatacattaa ctaaggaagt agtttcacac gaaggcttgg
     3301 cagatcgtat tgagaaaatg cttgaagaga ttcagcaaag tttgtttgat aaagcgaaat
     3361 cccgtttggt tgcgagtaca cgcaaggcag attcttacga tgcattcaaa aaaatgcttg
     3421 atgaagacgg tggatttatt cttgcacatt gggacggcac tcccgaaacg gaagaacgca
     3481 taaaagaaga gaccaaagca acaatccgtt gtattccatt ggatcaggaa aaagaagatg
     3541 ggaaatgtat ggtatcaggt aaaccttcga acgggagagt agtatttgca cgagcatatt
     3601 gattgaacta tggaaaacac ttcgccatct gccaacgatc tgatactgaa atcgctcaag
     3661 gagaagtcct cggttaagca ggatgtgttc tatctgtcga aagcccggtt ttccgaattc
     3721 aaaaaaattc tggcagaatt cgccaatctt gtaagtaaag aggcctccaa aatcgatgag
     3781 cgattaaaag ttgtatttac ggataaaggt gaatacgaag cacaattgca ggttgcggga
     3841 gatgttttgg ttttccattt gcataccaac gtctttttgt tcgatagcga tcattcactt
     3901 tggaagacat cctatctcaa agatgatgct agcagagggt attgcgggat aattcatgta
     3961 tataattttc tttctgattc gtttaaatac aacaggttag gagatttagg atacctggtt
     4021 gcccgtgttt ttgtgaaccg tgaaaaccat tttatggtac agggtaaacg gcaattgggc
     4081 tttttgtaca acgatttcat caattcccaa ttcagtccgg agcaaatgaa ggctgttgtt
     4141 gagtcgattg tgttgtacac catcgatttt gatttgttta ccccaccata cgacctgatt
     4201 aaggaagtta gtttaatgga aatcaaggag ttgagtgaca ccttgcagat gaagaccgga
     4261 aagcgtcttg gctttaaatt tcaggccgac tcagatgatt tttaatttct caacaatttt
     4321 tttcaccgat ttcttcgcgg ttaattttct ttttacatac atttgcactc ccaaaatgaa
     4381 gggatggccc gttcgtctag gggttaggac acaagatttt cattcttgga acaggggttc
     4441 gattccccta cgggctacta aaaatcttcc gggcaaccgg gtgaacattt aaagaaaaga
     4501 caatggcaaa ccacaagtca gcaatcaagc gtattcgttc aaacgacgcc aagcgtgttc
     4561 gtaatcgtta ttaccaccgc actacacgta acgctgttaa ggcaattcgt acttccaaca
     4621 ataagaaaga agctcttgga ttggtaagcg cagtattttc catgcttgat aagctggcaa
     4681 agcgcaatgt catccacaag aacaaagcag cgaacatcaa gtccagcatt caccaccacg
     4741 tgaacgcact ggcataattc caatacattg tttgagaatg atagggctcc cgaaacggag
     4801 ccctattgtt ttttactccc ggtccaaaca ctcagttaac accaaaattg gcctgcaact
     4861 gtctttttct tttacgtggt ttgtgcaatg gaagaacgaa aaaataccat tcgccaatgg
     4921 gcagaagacg accgtccgcg tgagaaacta attcgtttgg gacgcagagc gctttcggat
     4981 gctgaattgc tggccattct gatttcttca ggaaatgaac gtgaaacagc attggatctg
     5041 tcccggaggt tgttatcaga ggctgcagga aacagcttga accgtctcgc ccgattaagt
     5101 gttggcgatc ttcaaaaatt ccgggggata ggagaagcaa aagcgattac catacttgct
     5161 gcgcttgaat tgagcgttcg ccgcagagca gctgaaaagg aaatccgccc aactatttca
     5221 gggagtaaag acgcttttca gtatatcgaa cccttgttaa gtgatcttag ccacgaagaa
     5281 ttctatgtga tttaccttaa tcgcgcgaat gtagtaataa gccatgccag ggtaagttca
     5341 ggagggatta caggaaccgt tgtggatgca cgaatggttt ataaaggggt gctggatgcg
     5401 caggcaactt caattatcct ttgccataat cacccttccg gtaatctaag gcccagtaat
     5461 gaagatatct cccttacaaa aaaacttaag gaaggcggta aattacttga tataaatgtg
     5521 ctggaccatc tcatagtgtg taacaatgcc ttctatagtt ttgcggatga tggcctgatt
     5581 tgaattgtag tatacgaatt accctgtagg ttcgtacttt tagggtaatg aaaaagattc
     5641 ttaccatagt tggtgcccga ccgcaaatca ttaaagcagc agccctaagc agggttatcc
     5701 gtaatcagtt ttctgatgtt ttacaggaaa aaatcctgca cactggtcag cattacgacg
     5761 atagtatgtc cgccgttttt tttcgtgagc ttggcattcc tcaaccggat tttcagttaa
     5821 gtgtaggttc aggatcgcat gggcagcaaa ctgcaaaaat gctggaagga attgagaagg
     5881 tgttattgga cgaagcaatt gatgctcttg ttgtttatgg agacaccaac tctacggttg
     5941 caggagcact tgctgcgtct aagttgcgga ttccggttgt tcatattgag gcaggattaa
     6001 gatcgttcaa taaatcaatg cctgaagaaa tcaatcgaat tcttaccgat catgtttctt
     6061 ctttattgtt tac
//
LOCUS       DAOMYG010000163         6066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4427_length_6066_cov_9.956247, whole genome shotgun sequence.
ACCESSION   DAOMYG010000163 DAOMYG010000000
VERSION     DAOMYG010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4427_length_6066_cov_9.956247"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="PK610_06625"
     CDS             complement(<1..765)
                     /locus_tag="PK610_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRJ35642.1"
                     /translation="MKSIKYIFLLLLLPQQLLAQSVQFAARVSRNSLGVDENFQLEIA
                     TNTDGKVTLPDLSSFRIIGGPNRSSFSSTSFSNGQRIQVNTYSWTYWLMPVKEGEFTI
                     GAAKLIFEGKEYTTDPIKIKVGKAKDPSFYQDNSRQQIFISLRTNRSKVYEGEPVVVS
                     YTVNNRLMRASIEDHEFPNQTGCWMQIIDPGQKGWPSYTEKSEGMDYQVTIFKKEILY
                     PNSFGTLKLKPYKMKILGQDRFFTKQFDLNSNSASLE"
     gene            complement(765..1553)
                     /locus_tag="PK610_06630"
     CDS             complement(765..1553)
                     /locus_tag="PK610_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ35643.1"
                     /translation="MVKATLFTGIILLFLVPRAKGQDQEFLGRKYLREANAHYAKGEY
                     KEAMAKYQLALQNDPSNVKSGYNLGNAMYRDKNYSEAIKAYESNALQLHDPLQRAKAF
                     HNLGNAHMQAQDYQKAIDAYKRALKDNPNDEDTRYNLAYAQQMLKQQQQQNEKNKDQN
                     KDQNKDQNKDKNKDQNKDQNKDQNKDQNKDQNKDQNKDQNKDENKAENKKNGDQSKQQ
                     RPNQLSKEQAQKILEALNGNEKKLQDKINKKKEKAIVTGIEKDW"
     gene            complement(1523..2674)
                     /locus_tag="PK610_06635"
     CDS             complement(1523..2674)
                     /locus_tag="PK610_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRJ35644.1"
                     /translation="MNNASTYRVRTLVVLVLISELLFWSAAISLWLYLQTNVEEFRME
                     NVELLQLLYGLPVLGLSFLLFARWKNNALKRFAEHSLINRIDPGSSTSKSFLRFFLFR
                     SAMAMFIIALANPQYGKTKKEAVASGIDLMIALDVSNSMLAEDMNAEMNRLRVARLSI
                     EKLLGKLHGDRIGLVVFAGASYTQLPITTDYSAARLFLSGVSTNMMSSQGTAIGSAID
                     TCMRAFNSEDETKKAIIVISDGENHEDDAIGSAKRAESEGVIVHTIGVGSPEGSPIPV
                     YENGQKTGVKRDNEGNTVITKLNEAMLTEIANAGGGSYTRASGSDLGLDRVVRSISSM
                     EKKEYLAEMYTDYEDQFQIFIGLGLLLLVLESLITEKPNPWLRRLYSQA"
     gene            complement(2667..3695)
                     /locus_tag="PK610_06640"
     CDS             complement(2667..3695)
                     /locus_tag="PK610_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRJ35645.1"
                     /translation="MNKEASFDFGWLDFSWADKHLFWLLLVIPLLAVWYYYIHYKRSQ
                     AIRFSSLNGIASSGWNSIPALLFSLRLFALGVLILAFARPQLQEDETFTRKSAEGIDI
                     VMALDISGSMMAEDFKPNRLVVAKEVAINFIKERPNDRIGLVVYEGQAFTQCPLTTDH
                     NVLIRLFNDVEPGMIQDGTAIGMGLATAVNRLKESDGKSKVVILLTDGVNNQGNIDPL
                     TAAEIAREFGVRVYTIGVGKNGKAPFPAIDPFGRKVRTMMDVKIDEPMMKSIAEMTGG
                     KYFRAENRDKLESIYKEIDQLEKTLIKVTEFRVDPPEKFHSILLIGMLLLAVEFLLRH
                     FIFRMIHE"
     gene            complement(3688..4626)
                     /locus_tag="PK610_06645"
     CDS             complement(3688..4626)
                     /locus_tag="PK610_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35646.1"
                     /translation="MKHILVNILLICVPLFGFSQEVKLQLNLGEIRIGEPCKLNIEVR
                     LPNEKSIIGWPEFTDTFSTSKLEIISKSAPAMVNQLTGQMEYTVTSFDSGYHAIPPVS
                     VLIDGDSILSNALLIKVNTVEVDTTKQFRDIKNIEEEPFSWKDYFLSIWYWILDNWYI
                     VLSIVILTIALVLYIIRKRKKNELPPPPPLVAPHIEAIERLRIIEKEQQWQKGFYKDY
                     YTSLSDCLRHYIERRFNVAALEQTTDEVVRSLRLIDLDEESRRHLVYVLKLSDLAKFA
                     KEQPAPHENESALKRSFEFVQRTQSVEPEIPSKENE"
     gene            complement(4623..5495)
                     /locus_tag="PK610_06650"
     CDS             complement(4623..5495)
                     /locus_tag="PK610_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018943039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRJ35647.1"
                     /translation="MDTRELLKKVRKIEIKTKGLSEQIFSGEYHSAFKGRGMAFSEVR
                     EYFPGDDIRTIDWNVTARFNHPYVKVFEEERELTVTLLLDLSGSSDFGTKGSFKRELI
                     AEIAAVIAFSALKNNDKVGAIFFTDTVERFIPPKKGKTHILRIISDIISFEPKSKGTD
                     LAEPLRFLTNAIKKRSIAFVLSDFITENAFDDTLRIAARRHDIVALEIFDERELELPN
                     AGIIRLRDNESGKTRIINSSLRSVRTKYKANMLRRKDALKSRFRKAGVDHAAIATGSN
                     YIRPLMQLFKSRGK"
     gene            complement(5504..>6066)
                     /locus_tag="PK610_06655"
     CDS             complement(5504..>6066)
                     /locus_tag="PK610_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013620715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRJ35648.1"
                     /translation="TFKLPEPFLVLATQNPVEQEGTYPLPEAQVDRFMLKVVLTYPKK
                     EEEKLIIRANLAPEGMPKPNKVLTPEVILSARKLVREVYMDEKIEQYIVDIIHATRFP
                     AQYGLTKIEPFISFGGSPRASINLALAAKAYAFIRRRGYVIPEDVRSVCHDVLRHRIG
                     LTYEAEAENVTVESIINEILNRVEVP"
BASE COUNT         1591 a         1299 c         1222 g         1954 t
ORIGIN      
        1 ttccagtgat gctgaattgc tgttaaggtc aaattgtttt gtaaaaaaac gatcctggcc
       61 aaggattttc attttatagg gcttcagctt aagtgttccg aaggaattgg gatagagaat
      121 ttcttttttg aaaatggtga cctgataatc catcccctcc gatttttcag tataactcgg
      181 ccagcctttt tgtcccggat caatgatttg catccaacaa ccggtctggt tggggaactc
      241 atgatcttcg atggatgcac gcattagcct attgtttacc gtataactga caaccacagg
      301 ttctccttcg taaactttac tcctgttcgt gcgaagcgaa atgaagattt gctgccggga
      361 attgtcctgg taaaatgatg gatcttttgc cttgccaact ttgatcttga tgggatctgt
      421 tgtgtattct tttccctcaa aaatcaattt ggcagcacca atggtaaatt ctccctcttt
      481 aaccggcatt aaccaatagg tccagctgta ggtattaact tgtatgcgtt gtccattgga
      541 aaagctggtg ctggagaaac tacttctgtt tggaccacca attattcgaa atgaagacag
      601 atcgggtagg gttacttttc catctgtatt ggttgcgatt tccaattgaa aattttcatc
      661 cactcccaga ctgttacgcg aaactctggc ggcaaactgt acgctttgag cgagcagctg
      721 ctgaggaagc aataatagta agaagatgta tttaatgctt ttcattacca gtccttttca
      781 atgcccgtta caatagcctt ttcttttttc ttgttgatct tatcctgaag ctttttctca
      841 tttccgttta atgcttcaag aattttttgc gcttgttctt tgcttaattg attcggtcgc
      901 tgctgttttg actgatctcc atttttttta ttttccgctt tgttttcgtc cttgttctga
      961 tccttgttct gatctttgtt ctggtccttg ttttggtcct tgttttggtc tttgttctgg
     1021 tccttgtttt ggtctttgtt cttgtcctta ttctggtctt tgttttggtc tttattctga
     1081 tccttatttt tttcgttttg ttgctgctgt tgtttcagca tttgttgggc ataggcaagg
     1141 ttgtatcggg tatcttcatc gttgggattg tcttttaatg cacgcttata agcatcaatt
     1201 gctttctggt agtcttgcgc ctgcatatgt gcatttccca aattatgaaa cgcttttgcc
     1261 ctttgcaacg ggtcgtgaag ctgaagcgca ttgctttcat acgctttaat tgcttctgaa
     1321 taatttttat ccctgtacat cgcattacca agattgtaac ctgattttac gttggaagga
     1381 tcattttgta aggccaattg atacttggcc atcgcttctt tgtattctcc ttttgcataa
     1441 tgtgcatttg cttcacggag gtattttctt ccgagaaatt cctgatcctg accctttgca
     1501 cgggggacaa gaaacagcag gattatgcct gtgaataaag tcgccttaac catgggttgg
     1561 gtttttcagt gattaaactt tccaatacca ataacaataa tccaagtcca ataaaaatct
     1621 ggaactgatc ttcgtaatcg gtatacattt ctgccaggta ttctttcttt tccattgagg
     1681 aaatactgcg taccacacga tccaatccaa ggtcactgcc acttgctctg gtataacttc
     1741 cgcctccggc attggcaatt tcagtaagca ttgcttcgtt cagtttggtt ataacagtat
     1801 tcccttcatt gtcgcgctta acccctgttt tttggccgtt ttcataaaca ggaattggac
     1861 taccctcagg cgatccaacg ccaatggtgt gcacaatcac accttctgat tcagctcgtt
     1921 ttgctgaacc aatggcatca tcttcgtgat tttctccatc tgaaattaca atgattgctt
     1981 ttttcgtttc atcttccgaa ttgaatgcac gcatacaagt atcaatagcg ctgccaatgg
     2041 ctgtgccctg tgaactcatc atgtttgtgc taacgccgct caaaaagagt ctggcagcag
     2101 agtaatcggt agtaattgga agttgtgtgt aggaagctcc ggcaaaaaca actaatccaa
     2161 tacgatcgcc atgtaatttc cccaataatt tctcgatgga aagacgggca acacgtaagc
     2221 gattcatttc cgcattcatg tcttcagcca gcatactgtt ggagacgtcc aatgcaatca
     2281 tcagatcgat tccggatgca acggcttctt ttttggtttt tccatattga ggattggcaa
     2341 gtgctatgat aaacatggcc atagcacttc tgaaaaggaa aaagcgaaga aaagattttg
     2401 aagtggaact accgggatcg atcctgttga tcagactatg ttctgcaaaa cgttttaacg
     2461 cattgttctt ccatctggca aaaagtaaaa acgacaaacc caaaacaggt aatccgtaca
     2521 gcaattgtaa aagttcaaca ttttccatcc ggaactcctc tacattcgtt tgcaggtaca
     2581 accaaagact gattgcagca ctccagaata aaagttcact gatcagcaca aggacgacca
     2641 atgttcgaac gcgatatgta ctggcattat tcatggatca tacggaaaat aaagtgtctg
     2701 agcagaaact ctacagcaag aagcagcatg ccgatcaata aaatcgagtg aaatttttca
     2761 ggaggatcaa ctctgaattc ggttactttt attagcgtct tttctaattg atcgatctct
     2821 ttgtaaatgg attccagctt atctctgttc tctgccctga agtattttcc tccggtcatt
     2881 tcggcaatcg acttcatcat cggctcgtcg atttttacat ccatcatcgt gcggactttt
     2941 ctaccgaacg gatcaattgc agggaaaggt gcttttccat tttttccaac accaatcgta
     3001 taaaccctta ctccaaattc tcttgcgatt tctgctgcag taagcggatc aatatttccc
     3061 tggttattta caccatcggt taacagaatt acaactttac tttttccatc actttctttc
     3121 aacctgttca ctgcagtcgc taaccccatt ccaatggcag taccgtcctg aatcattccg
     3181 ggttctacat cattaaaaag acgaatcaac acattgtggt cggtggttaa cggacattga
     3241 gtaaatgctt ggccttcata aactaccagt ccaattctgt cattaggacg ttccttaata
     3301 aaattaatgg cgacttcctt tgcaacaacc aggcgatttg gtttgaaatc ttctgccatc
     3361 atactccctg aaatatccag tgccatcaca atatcaattc cctctgcaga tttacgggtg
     3421 aaggtttcat cctcctgcaa ttgcggacgg gcaaaggcaa ggatgagaac acccaaagcg
     3481 aatagacgaa gcgaaaaaag tagtgcagga atactattcc atcccgaaga tgcaatccca
     3541 ttcagtgaag agaaacgtat tgcctgactg cgtttataat ggatgtagta ataccaaaca
     3601 gcaagtaaag gaatgaccaa gagcaaccaa aataggtgct tatctgccca tgaaaaatcg
     3661 agccagccaa aatcgaagct tgcttcttta ttcattttcc tttgatggta tttccggttc
     3721 taccgattgt gtgcgttgca caaattcaaa cgaacgtttc aatgccgatt cgttttcatg
     3781 tggcgcaggt tgttcctttg caaacttggc gaggtccgat aatttcagaa catacaccaa
     3841 atgtcgtctc gattcttcgt ccagatctat tagtcgaagt gatcgcacaa cttcatcagt
     3901 agtttgttca agggctgcaa cattaaaacg acgttcaata taatggcgta aacaatcgga
     3961 taatgaagta taatagtctt tgtagaatcc tttttgccac tgctgttcct tttcaattat
     4021 tcgcaatcgc tcaattgctt caatatgggg tgcaacaaga ggtggaggag gagggagctc
     4081 gttctttttt cgtttgcgaa tgatgtatag aaccaaagca atggttagaa ttacaatgga
     4141 aaggacgatg taccagttgt ccaatatcca ataccaaatg cttaaaaagt aatctttcca
     4201 ggagaagggt tcttcttcaa tatttttaat atcccggaat tgtttagtag tatcaacttc
     4261 aacggtattt accttgatca acagcgcatt tgataaaata gaatctccgt cgattaaaac
     4321 actgaccggt ggaatagcgt ggtaaccgct gtcgaacgat gtgactgtgt attccatttg
     4381 tccggttagc tggtttacca ttgccggagc agattttgaa atgatttcaa gctttgaagt
     4441 actgaatgta tctgtaaatt ccggccatcc gataattgat ttttcattgg gaagacggac
     4501 ttcaatatta agtttacagg gttctccgat tcggatctct cccagattta attgaagttt
     4561 tacttcttgc gaaaatccga acagcggaac acagatgagc aatatgttta caaggatgtg
     4621 tttcatttgc ccctgctttt aaacaattgc attagcggac ggatataatt acttcctgta
     4681 gcaattgcag cgtgatcaac tccagcttta cggaatcggc ttttgagcgc gtctttgcgc
     4741 cgtaacatgt ttgccttgta tttagtgcgg acagatcgaa gtgatgaatt gatgattctg
     4801 gttttgccac tttcgttgtc gcgcaaacgg atgattcccg cattgggtaa ttccaactcg
     4861 cgttcgtcaa aaatttcgag cgctacaata tcatgccgtc gcgccgcaat acgcaaggtg
     4921 tcgtcaaatg cattttcggt aatgaaatcc gacaatacaa atgcaatact acgtttctta
     4981 atggcattgg tgagaaagcg taatggttct gccagatctg ttcctttgct tttaggttca
     5041 aaactgataa tatcgctgat gattctcaga atatgagttt tccctttttt tggaggaata
     5101 aatcgttcaa cagtatcggt aaaaaaaatc gcgcccacct tatcattgtt tttcagagca
     5161 gaaaatgcaa tgactgccgc aatttctgca atgagttcgc gcttaaagct gccttttgtt
     5221 ccaaaatcgg atgagcccga aagatccaga agaagtgtca ctgtaagttc acgttcttct
     5281 tcgaatactt taacataagg atgattaaac cgtgctgtta cgttccagtc gattgtacgt
     5341 atatcgtcgc ccggaaaata ttcccttact tcactaaagg ccattcctct tcccttaaag
     5401 gcagaatggt attcgcccga aaagatctgt tcgctcaagc ccttggtctt gatttcgatc
     5461 ttccgcactt tcttcagtaa ctcccgggtg tccagcatag caatcaggga acctctacgc
     5521 gattaaggat ttcgttgatg atgctttcca ccgtaacatt ttctgcctcg gcttcatagg
     5581 tgagaccgat acggtgacgt aaaacatcgt ggcaaactga acgtacatct tctggtataa
     5641 catagcctct tctgcgaatg aatgcatatg cttttgctgc cagtgccaaa ttgatggatg
     5701 cccgaggaga accaccgaac gaaatgaaag gttcaatttt agttaaaccg tattgcgcag
     5761 gaaaacgggt ggcatgaatg atgtcaacaa tgtattgctc aattttttca tccatataaa
     5821 cttcacgaac caatttcctg gctgaaagaa tcacttctgg ggtaagtact ttattgggct
     5881 tgggcattcc ctcaggtgca agattggcac ggatgatcag tttctcctct tcctttttgg
     5941 gataagttag aactaccttt aacataaaac gatcgacttg tgcttcaggt agtggatagg
     6001 ttccttcctg ttctaccggg ttttgggttg ccaatacaag aaagggttcg ggtagtttaa
     6061 aggtat
//
LOCUS       DAOMYG010000164         6054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4436_length_6054_cov_9.669278, whole genome shotgun sequence.
ACCESSION   DAOMYG010000164 DAOMYG010000000
VERSION     DAOMYG010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4436_length_6054_cov_9.669278"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PK610_06660"
     CDS             <1..843
                     /locus_tag="PK610_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35649.1"
                     /translation="DRIVDVCWIDGLNGEDPLRSAYYLAAPQNVAVTHNSGQAAGEQF
                     RMKPYVQLLSSNRAIEDFLPKEGVAKTEGSFRFVPVGVVKEKALLSATWFDISFGDVA
                     LEGEKNYNNLRGEIIGSYDLMTGVPVDVVMKNKSNGFLRLETEGISVTYMVVNGKVGD
                     LYSSWFKRRDNKHLISYTRTIISGILLGDVAEFNEMIGNTTNVKPYVIRSGAMEEVRT
                     ALKDWTGEIESLTKITGTSELIPEGETHVYSVLIPGQIELLFSGEGSGGYGLRSIRAP
                     FIKK"
     gene            complement(864..1604)
                     /locus_tag="PK610_06665"
     CDS             complement(864..1604)
                     /locus_tag="PK610_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35650.1"
                     /translation="MKKIILICIILKTQLAFGQNKTEISFGISNPGQVEKTLEDFYFI
                     AHPTAFQDDAHYGNEKRVKFNLGFSFHLRDSLELRLRVGYSKRVNHYSQAFSNTHWSV
                     NDFQSVFEISPSFGFSKRLGRFRFSTGIEIPVYIIGKLKEDLWYQTYASSGTIDLAST
                     SEISMPGGTIIGLNNYVRVSTFLSKQLFLYSEINYGLLFASLGGNFRNVTTSTFPIEE
                     TLINEFEKTYTKWYFSPAQIQFGIGLKF"
     gene            complement(1749..2228)
                     /locus_tag="PK610_06670"
     CDS             complement(1749..2228)
                     /locus_tag="PK610_06670"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRJ35651.1"
                     /translation="MEEEIRKKKRYTPQQAWPLIQNFCAYRERCHQEIRDKLYSYSLT
                     SDDVENIIVKLIEEKYLDEERYARAFVSGKYRIKKWGRSRIVRELKMRKISEYCIKKG
                     LEEIDPDEYYENLKHLVEKKYNSIKGDHPAIKRKKTAHYLMTKGYEGDLVWDVVKEI"
     gene            complement(2232..2855)
                     /locus_tag="PK610_06675"
     CDS             complement(2232..2855)
                     /locus_tag="PK610_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ35652.1"
                     /translation="MQITLNTAGRRFNKEWIFRGLSRTFNSGESIGIIGGNGSGKSTM
                     LQVVSGFLIPSEGSITYNHETKQIDNETIYRYVSMASPFLELYEEYTASELFAFHSAL
                     KPMRDKIDAALFLETLQLENIRNKALKNYSSGMKQRVKLALAILSDTPLLLLDEPCSN
                     LDKKAISWYQSLLLAHSENRIVLVSSNSDENELFNCTERLSVEDYKR"
     gene            complement(2870..3646)
                     /gene="lpxA"
                     /locus_tag="PK610_06680"
     CDS             complement(2870..3646)
                     /gene="lpxA"
                     /locus_tag="PK610_06680"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRJ35653.1"
                     /translation="MSQPTANIHPDAKIGKGTVVEPFATIMNDVVIGDNCHIGPNVVI
                     YPATRIGNNVKIFPGAIIGPDSQDLKYAGEYTTTEIGDNTVIREYVTIHRGTKDRMKT
                     VVGSNCLLMAYVHVAHDCIVGNNVIIANSVGLSGHVTIDDHAIIEGMAAVQQFIHIGA
                     HSFIGGGTMVRKNVPPFVKAAREPISFIGVNTVGLRRRGYSDFQITQIEDIYRIIYVQ
                     NSNVTTALKIVEFELPASEEKDQIVTFIKNSTKGIMRGIS"
     gene            complement(3643..5052)
                     /locus_tag="PK610_06685"
     CDS             complement(3643..5052)
                     /locus_tag="PK610_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018675890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HRJ35654.1"
                     /translation="MKEFQRTLKAPAKLSGIGLHTGAPVNITILPASDNHGYKFQRMD
                     VEGQPIIKADADLVISTERGTTLEHNGVRVHTTEHLLAALYGMQVDNALIQIDGPEIP
                     IMDGSSKPFVAAIEAVGYEQQKAEREYFEFKENLPWEDLEKGIEMLAVPAQDFRLTVM
                     VDYNSPVLGTQHATMYNLNEFKENIASCRTFVFLRELEYLAKKGLIKGGAPDNAIVLV
                     DREEVNENELKELASLLGKEDLKIQFKGIGILNNTKMQYENEPARHKLLDIVGDLALV
                     GKPIKGHILAARPGHSGNISFAKIIKDIMRAEKKAGVVKHFDLTKEPLHDIYKIEKML
                     PHRYPFLLVDKIMSMTENGIVGVKNVTMNEPYFMGHFPGNPVMPGVLQVEAMAQCGGI
                     FALSSVPDPENWSTYFMKIDGVKFKRKVIPGDSIVFDLELITPIRRGIVHMRGTAYVN
                     GQVASEAEMMAQIVKERNL"
     gene            complement(5049..>6054)
                     /gene="lpxD"
                     /locus_tag="PK610_06690"
     CDS             complement(5049..>6054)
                     /gene="lpxD"
                     /locus_tag="PK610_06690"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009780154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRJ35655.1"
                     /translation="EGNPDATVSNLAKIEEGTPGTLTFLANPKYAEFIYSTGASVAIV
                     GIDFKAEKALPDQLTLIRVAEPYSSFAKLLEAYNQFRGDKKGIEQPSFIDEQASVGED
                     VYIGAFAYVGKNARIGKGAKIYPHCYIGDNVRIGENTTIFPGVKIYHDCIIGANTTIH
                     ASSVIGADGFGFAPNSENNYAKVPQIGNVVIEDHVEIGSNVSIDRATLGSTVIRRGVK
                     IDNLIQIAHNVEIGENTVIAAQTGVAGSTKLGKNMMVGGQVGIVGHLRIADGVKIAAQ
                     SGIGQNIEEKDAIVQGSPAFGIGDYKRSYVLFRSLPQLREQINTLAKEIKSLKQDPNS
                     "
BASE COUNT         1617 a         1262 c         1262 g         1913 t
ORIGIN      
        1 gatcggatag tggatgtgtg ctggatcgat ggactcaatg gagaggatcc tctccgttcg
       61 gcttactacc ttgccgcccc gcaaaatgtg gctgtaacac ataatagcgg gcaagctgct
      121 ggtgaacaat tcaggatgaa accttatgtt caattgcttt catccaatcg cgctatcgaa
      181 gattttcttc ccaaagaagg tgttgcaaaa acagaaggta gttttcgctt tgttcctgtt
      241 ggtgttgtta aggaaaaggc tctgctttct gcaacctggt tcgatatttc ttttggtgat
      301 gtggcacttg aaggagaaaa gaattacaat aaccttcgcg gcgaaatcat cggttcatat
      361 gatttaatga ccggtgttcc tgtggatgtt gtaatgaaaa acaaaagcaa tggttttctc
      421 cgactcgaaa cagaaggaat tagcgttacc tatatggtcg taaatggaaa agttggtgat
      481 ttgtattcat cctggtttaa gcgtagggat aacaaacacc tcatctccta tacgcgcacc
      541 attatttcgg gaattcttct gggtgatgta gctgagttta atgaaatgat cggaaacacc
      601 accaatgtaa aaccttatgt aattcggtcc ggggcaatgg aggaagtacg cactgcgctt
      661 aaggactgga ccggagaaat tgaaagcctt accaaaataa ccggaacttc tgagctcatt
      721 cctgaaggcg aaacccacgt gtattcagtt ctcattcccg gacaaattga attacttttt
      781 tccggagagg gtagcggtgg ttatggtttg cgtagtattc gcgcaccttt catcaagaaa
      841 tagttgaacg gaaagcgttt aactcaaaac tttaatccga tcccaaattg gatctgagca
      901 ggtgaaaaat accatttcgt ataggttttt tcgaattcat taattaatgt ttcttcaatg
      961 ggaaatgttg atgtggttac attacggaaa ttgcctccta agcttgcgaa taaaagaccg
     1021 taattaattt ctgaatagag gaacaattgc tttgaaagaa atgtgctaac tctaacgtaa
     1081 ttattcaaac caataatagt accgccaggc atgcttattt cagaggttga agccaaatct
     1141 attgtgcccg aagaagcata ggtctggtac cagagatctt cttttagttt tccgataata
     1201 tataccggaa tttcaatccc ggttgaaaat ctaaatctac caagacgttt cgaaaaacca
     1261 aaggaaggac tgatttcaaa gacagattga aaatcattga ccgaccaatg ggtgtttgaa
     1321 aatgcctgtg aataatggtt cactcttttt gaatatccca ctcgcaaacg cagttcaaga
     1381 ctgtcgcgca aatgaaaact gaaaccgaga ttgaatttta cccgtttttc atttccataa
     1441 tgtgcgtcat cctgaaatgc agtcggatgc gcaataaaat aaaagtcttc aagcgttttt
     1501 tctacttgcc ccggatttga aattccaaag ctgatttctg ttttattttg accaaaagct
     1561 aattgggtct ttaatataat gcagattaga attatttttt tcatgccgta aattattgaa
     1621 tttcgcgttg tgtactattg taaatataca aagttgaaac agttgaacaa ttttttccaa
     1681 ttcagtgccg ctgcatgatt cgaaaaaaga atatgtaaaa cttccgttct tcaccaaagg
     1741 tatcttactc aaatttcctt cactacatcc caaaccaaat ctccttcata gccttttgtc
     1801 atgaggtaat gcgctgtttt ttttcgctta attgcaggat gatcgccttt tatggaattg
     1861 tatttttttt ctacaaggtg ttttaaattc tcgtaatatt catccggatc aatttcttcg
     1921 agtccttttt tgatgcagta ctcactgatc tttctcattt ttagttcgcg tacaattcgg
     1981 cttcgacccc attttttaat gcggtatttt ccgctcacaa atgcccgtgc atatcgttct
     2041 tcatcaaggt atttttcctc aattagtttc acaatgatat tctctacgtc atctgaagtt
     2101 aaggaatagg agtagagctt atcgcggatt tcctgatgac aacgttctct gtaagcacag
     2161 aagttttgaa tcagcggcca ggcttgttgc ggagtgtagc gttttttttt acgaatctct
     2221 tcttccacct ttcagcgttt ataatcctcc acagaaagcc tttcagtgca attgaaaagt
     2281 tcattttcat cgctgttgga tgaaaccaat acaatgcggt tttctgaatg agcaaggagt
     2341 aatgattggt accaggaaat ggcttttttg tcaagattag aacaaggctc atcgagaagc
     2401 aggaggggtg tgtcggatag aatcgcaagt gcgagtttca ccctttgttt cattcctgaa
     2461 gaataatttt ttaaggcctt attgcgaata ttttccaatt gaagtgtttc aagaaacaat
     2521 gctgcatcta ttttatcccg catgggtttt aatgcggaat gaaaggcaaa caattctgaa
     2581 gctgtatatt cttcataaag ctcaagaaag ggtgaagcca ttgatacata tcgataaatc
     2641 gtttcgttat cgatttgttt tgtttcatga ttgtaagtga tactgccttc agaaggaatt
     2701 aaaaaaccac tgactacctg aagcatggtc gattttccgg atccgtttcc tccaattata
     2761 cctatgcttt caccgctgtt aaaagtccgc gacaaaccac ggaagatcca ctctttgttg
     2821 aatcgtcttc cggctgtgtt gagggtgatt tgcataggtg aaactgcgct caggaaattc
     2881 cgcgcatgat gcctttggtg gaattcttaa tgaaggtgac aatctgatct ttttcttcac
     2941 tcgcaggtaa ttcgaattct acaattttca atgcagtggt tacattgctg ttctgaacgt
     3001 aaattatccg gtaaatatct tcgatctgtg taatctgaaa gtcagaatat cctctgcgac
     3061 gaagcccaac agtgttcact ccaataaagg agatgggttc acgtgctgcc tttacaaatg
     3121 ggggtacatt tttacgaacc atcgttcctc caccaatgaa ggaatgtgca ccaatatgaa
     3181 tgaactgctg taccgcagcc attccttcaa tgatggcatg atcgtcgatt gtaacgtgtc
     3241 cggataatcc tacactgttt gcaatgatca cattgttgcc aacaatacag tcgtgcgcaa
     3301 cgtgtacata ggccataagc aggcaattgc ttcccactac cgttttcatt ctgtctttgg
     3361 ttccgcgatg gatcgtgaca tattcgcgga tcacggtgtt gtcgccaatt tctgttgtgg
     3421 tgtattcacc tgcgtatttc agatcctgag agtcgggacc aataatcgct cccgggaaaa
     3481 ttttcacatt gtttccgatg cgtgttgcag gatagatcac aacgtttgga ccaatatgac
     3541 aattgtcgcc aatcacaaca tcattcatga ttgttgcgaa tggttcaact actgtgcctt
     3601 tgccaatctt ggcatcagga tgaatatttg cagttggctg actcataaat ttctttcttt
     3661 gacaatctgt gccatcattt cagcttccga ggcaacttgt ccgttaacgt aggctgttcc
     3721 tctcatgtgt acaattcctc ttcgaatcgg agtaatgagc tcaagatcga atacaattga
     3781 atcgccggga atgactttgc gtttgaattt tacgccatca attttcatga agtaggtcga
     3841 ccaattttcc gggtctggga cagaacttaa tgcgaaaatt cctccacact gagccatcgc
     3901 ttcaacctgc agaactccgg gcataacagg gttgccggga aagtgtccca tgaagtaggg
     3961 ttcattcatg gtaacatttt ttacacccac aattccgttt tctgtcatcg acatgatttt
     4021 atcgacgagc aggaaaggat atcggtgcgg caacattttt tcaatcttgt agatatcatg
     4081 caaaggctct ttcgtgagat caaaatgttt cacaacgcct gcttttttct cagcacgcat
     4141 gatgtccttg atgatttttg caaatgaaat attaccactg tgaccgggac gggcagcgag
     4201 aatatgtcct tttattggtt tacccaccaa cgcaagatca cctacgatgt ccagcagttt
     4261 atgtcgcgcc ggttcgtttt cgtactgcat ttttgtgttg ttgagaatgc caatgccttt
     4321 gaattggatt ttcagatctt cttttccaag taatgaagca agttctttca attcattttc
     4381 attcacttct tcacgatcca ccaaaacaat ggcattatcg ggtgcgccac ctttgatgag
     4441 tccctttttt gcaaggtatt cgagttcgcg cagaaaaacg aaagttctgc aggaggcgat
     4501 attttccttg aactcattga ggttgtacat cgtggcgtgc tgtgttccca atacaggaga
     4561 attgtaatcg accattacag tgagacgaaa atcctgcgcc ggtactgcca gcatttcaat
     4621 tcctttttca agatcttccc aaggaagatt ttccttgaat tcgaagtatt cgcgttctgc
     4681 tttttgttgt tcgtatccaa ctgcttcgat ggctgcaaca aaaggtttag aagacccatc
     4741 cataatggga atttcaggac catcaatttg tataagcgca ttatcaacct gcattccgta
     4801 caatgcagcg agcaaatgtt cggttgtgtg tacccgaacc ccattgtgtt caagtgtagt
     4861 acctctctcc gtagaaatta ccagatcggc atctgctttt atgatgggtt gtccttccac
     4921 atccattcgc tggaatttgt agccgtgatt gtctgatgcc ggaagtatgg tgatgttaac
     4981 gggagcgccg gtatgtaatc cgattccgga tagttttgct ggtgctttca gtgtgcgttg
     5041 gaattctttc atgaattcgg atcttgttta agcgatttga tttccttggc taaagtattg
     5101 atttgttcgc gaagctgtgg cagggagcga aacagaacgt aggaccgttt gtaatcccca
     5161 attccaaagg caggagaccc ttgaacaatg gcgtcttttt cttcgatgtt ttggccaata
     5221 ccggattgtg cggcgatttt aacaccatcg gcaattcgta agtgaccaac aattccaact
     5281 tgtccgccca ccatcatgtt tttaccaagc ttggtgcttc ccgctacgcc ggtttgtgct
     5341 gcgattacgg tattctctcc aatctcaaca ttgtgtgcga tctgaataag attgtcgatc
     5401 ttaactcctc ttcggataac tgtagaacca agtgttgctc tgtcgataga aacattggag
     5461 ccgatttcaa catgatcttc gatcaccaca ttcccgatct gaggaacttt ggcataattg
     5521 ttttcgctgt tgggtgcaaa accaaatcca tctgcaccga tcactgagga tgcgtgaatg
     5581 gtagtgttgg ctccaataat gcaatcgtga tagattttta caccgggaaa aatcgtggtg
     5641 ttttctccaa tgcggacatt atcaccgata tagcaatgcg gataaatttt tgctcctttt
     5701 ccaatgcggg cattctttcc tacataggca aatgcgccaa tgtaaacatc ttccccaacg
     5761 gacgcttgtt catctataaa gctgggttgt tctattcctt ttttgtctcc gcgaaactga
     5821 ttgtaggctt caagtaattt tgcgaaactg gaataaggct cagcaacgcg aatcagagta
     5881 agctgatctg gaagtgcttt ctccgctttg aaatcaattc ctacaattgc aacagatgct
     5941 cctgtagaat agatgaactc tgcatatttg ggattggcaa gaaaagtcaa agtacccgga
     6001 gtaccttctt caatctttgc aagattgctt acagttgcat caggattccc ctct
//
LOCUS       DAOMYG010000165         6035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4449_length_6035_cov_8.528595, whole genome shotgun sequence.
ACCESSION   DAOMYG010000165 DAOMYG010000000
VERSION     DAOMYG010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4449_length_6035_cov_8.528595"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..552)
                     /locus_tag="PK610_06695"
     CDS             complement(142..552)
                     /locus_tag="PK610_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007097056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRJ35656.1"
                     /translation="MNCTLSRKASFNAAHRLHNPKWSAEKNQEIFGICNRPNYHGHNY
                     TLIVSVTGEIDPETGYVMDTKILADLIQEHVEERFDHKNLNLDVAEFRDLNPTAENIA
                     VVIWNILRPHIVNSMQLKITLYETERNSVEYGGK"
     gene            complement(555..1106)
                     /gene="idi"
                     /locus_tag="PK610_06700"
     CDS             complement(555..1106)
                     /gene="idi"
                     /locus_tag="PK610_06700"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013188467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HRJ35657.1"
                     /translation="MNLPEQEYVILVDRNDQQVGTMEKMEAHRLGALHRAFSVFVFNS
                     NGEMLLQQRALGKYHSPGLWTNTCCSHPRPEEKTPDAAQRRLSEEMGFVTPLEKAFSF
                     IYKVQFENDLFEHELDHVFIGEYDEEPKINPEEVAAYRWTHPDEVMQEMQDDPELFTA
                     WFRICFEEMYDWYNAHLKNKKAS"
     gene            complement(1117..1623)
                     /locus_tag="PK610_06705"
     CDS             complement(1117..1623)
                     /locus_tag="PK610_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35658.1"
                     /translation="MRIVLLFLFTCFTFTIHANELNKIRALYFEAGKDSKKSEQLIKA
                     IPEDKAAKDPLQKAYRGAALTISADNSMNPFEKLNVFNKGKALLEEAIKTEPANTEMR
                     FLRFSVQSECPSFLNYSMNISEDKNHLLNHWKTFSQQFAGSTFLNNVREYLKASKKLS
                     AEEKAKLS"
     gene            complement(1623..2465)
                     /locus_tag="PK610_06710"
     CDS             complement(1623..2465)
                     /locus_tag="PK610_06710"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HRJ35659.1"
                     /translation="MADKAFFDTVSLRCSKMTTRAYSTSFSMGIRFLNKRFHDPIYAI
                     YGFVRFADEIVDTFHDYDKARLLEKFRKDTYEAIEEKISLNPILNAFQEVVNRYRMPR
                     HLIDTFLKSMEMDLNQNVYDQSGYEEYILGSAEVVGSMCLKVFVEGNEKLYNELEPYA
                     MKLGSAFQKINFLRDLRDDVNALGRVYFPGVDMSEFNEEVKKQIEKDIEVDFARGYEG
                     IQKLPKDSRFGVYVAYVYYYRLFAKIKALPANRILQERVRIPNGKKYSLFVTSYVRHS
                     FNLI"
     gene            complement(2469..3956)
                     /gene="crtI"
                     /locus_tag="PK610_06715"
     CDS             complement(2469..3956)
                     /gene="crtI"
                     /locus_tag="PK610_06715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HRJ35660.1"
                     /translation="MQKTAIVIGAGFAGLSTASCLAKLGMKVTLLEKNEQTGGRARVY
                     RKDGFSFDMGPSWYWMPDVFEKYFALFDKKPSDYYELERLSPSYRIYFAPNDFMDVPS
                     DMKELEAMFESYEKGAGEKLKKFLADGGYKYDVGVNDLVYRPGLSITEFLDKRVMGGL
                     FKMHLLKSFASYIRKYFSHPRLLQLLEFPVLFLGAKPENTPALYSLMNYADMQLGTWY
                     PKGGMMKIVEGMTSLAKEKGVDVRLNTPVTRLETKNGKIVAVHTLNEILTADVIVASA
                     DYHHIEQKVLDKESRKYTENYWDKRVLAPSSLIFYLGLNKKLNGMLHHTLFFDEDFGQ
                     HAKEIYDNPQWPSKPLFYTSCPSVTDPTIAPEGHEAFFILIPVAPGLNDTEEIREKYF
                     NLVVDRFEHLTNQKIREHIVLKRSYAHKDFIGDYNAFKGNAYGLANTLRQTAILKPKM
                     KSKKVSNLYYTGQLTVPGPGVPPTLISGQVAAKEIAKQFHLPTKL"
     gene            complement(3958..4467)
                     /locus_tag="PK610_06720"
     CDS             complement(3958..4467)
                     /locus_tag="PK610_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRJ35661.1"
                     /translation="MTAIEFNQHVIMMRPRLKAFAVSLTSDQEDAKDLLQDTILKAFT
                     YREKFTDSTNLGAWLFTIMKNTFINNYRRRKKANTIIDGTKDLHFVNIPQTKGSISPE
                     STYSENELLKGISELSDEYRIPFNMHLEGHKYKDIADEVGVPIGTVKSRIFLARQILM
                     KKFKDYRYN"
     gene            complement(4827..>6035)
                     /locus_tag="PK610_06725"
     CDS             complement(4827..>6035)
                     /locus_tag="PK610_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ35662.1"
                     /translation="VAVEPPPAWRVQTPEPTRKSMSSIYIPEPCHENWAAMSPTEKGR
                     FCAICTKEVVDFTRKTEKDILQHLEQAEGKTCGVFLPSQLSESHMKAKPATAREFRVK
                     KWLAASLAFLGLFNLGKEAKAQKMGKVAIKGDVMPIETHNANTQPSIIHGQVTTPDGK
                     AVAQATVIINSGDEQIGEIKTMANGSFRIEIAAGKIKNNSISISASHDFRYKYLEQVY
                     INKASTRVGIVLEEEYALLGEVAWVEPQENIDTIPHAEYTTVVEESLITKVDSTFVSC
                     NVIMGDTVLVHENLPEEIVAPDILEKVEEVIVDAAIPLEERQPETEIVQLEHTRIYPN
                     PGKEQLFIESDTDALHQVTISDLQGKIIHNEQFSGMRTSFNGSTLAPGIYLIRIQTGE
                     QSETHRWVVR"
BASE COUNT         1564 a         1270 c         1308 g         1893 t
ORIGIN      
        1 agtgtttcca taatggcccg gaaatggtga gtgatggcag tgatcttttc ttcatcgctt
       61 aattcgaagg catcatcacg cagtggtgtt tcgatgccgg ttaaaaaatg ttcgttgcct
      121 tcttcttcgt aaacaagcag attattttcc gccgtattca acactgttcc gttcagtttc
      181 atagagtgtg atcttgagtt gcatactgtt aacaatatgc ggacgcaaaa tgttccagat
      241 cactacagca atattttcgg cagtgggatt aagatcacga aattccgcaa catcgaggtt
      301 gaggttttta tgatcaaagc gttcttcaac gtgttcctga ataagatcag caagaatttt
      361 ggtgtccatc acataacccg tttcaggatc gatctctccg gttaccgaaa caatgagggt
      421 gtaattgtgt ccgtggtaat ttggtcggtt gcagattccg aagatctcct gatttttttc
      481 agcgctccat ttcggattgt gcaaacgatg tgcagcgttg aaggatgctt tacgggatag
      541 ggtacaattc attttcagga tgcttttttg ttttttaaat gggcattgta ccaatcgtac
      601 atttcctcga aacagatccg gaaccatgca gtgaacagtt caggatcgtc ctgcatttcc
      661 tgcatcactt catcgggatg tgtccagcgg taggctgcta cttcttccgg attgatcttc
      721 ggctcttcat catattctcc aatgaaaaca tgatcgagtt cgtgttcaaa cagatcgttt
      781 tcaaattgaa ctttataaat gaaactaaag gctttttcca acggtgtaac aaatcccatc
      841 tcttcactca ggcgacgctg tgcagcatcc ggagtttttt cttccggacg aggatgtgaa
      901 caacaggtat tggtccataa accgggcgaa tggtattttc cgagcgctcg ttgttgcaat
      961 agcatttcac cattgctgtt gaacacaaat acagaaaatg ctctgtgtag cgctccgaga
     1021 cgatgggctt ccattttttc catggtgcca acctgttggt cattacgatc aacaagaatt
     1081 acatattcct gttcgggaag gttcattgga tgcaatttac gaaagtttgg ccttttcttc
     1141 ggcggaaagt ttttttgagg ctttcaggta ttcccttaca ttattcagaa acgtgctgcc
     1201 ggcaaattgc tgactgaatg ttttccagtg gttaagcaaa tgatttttat cctcgctgat
     1261 gttcatggag taattcaaaa acgaaggaca ttcagactgc acactaaatc gcaaaaatcg
     1321 catttcggta ttggctggtt ccgttttgat ggcttcttcc aaaagtgctt tccctttgtt
     1381 gaacacattc agtttctcaa acggattcat ggaattatca gctgaaattg ttaatgcagc
     1441 acctcggtag gctttttgta aaggatcttt agctgcttta tcttccggga tagccttgat
     1501 cagttgttca ctctttttag aatcttttcc tgcttcaaaa tacaaggcac gaattttatt
     1561 cagctcgttg gcgtgaatgg taaacgtgaa acaagtaaag aggaagagta aaactattcg
     1621 catcagatca aattaaaact gtgacgaaca taggaggtaa caaacaaact gtattttttc
     1681 ccattgggaa tacggacccg ttcctgcaga atcctgttag caggcagcgc ttttatcttt
     1741 gcaaacaggc ggtaataata tacataagca acataaacgc caaagcggga atcttttggt
     1801 agtttctgta tgccttcgta gccacgtgca aagtcaactt cgatgtcttt ttcaatttgt
     1861 tttttgactt cctcattgaa ttccgacata tctacccctg ggaaatatac acgtcccaat
     1921 gcgttaacat catcacgcag atcgcgcagg aagttgattt tttgaaaggc agaacccagt
     1981 ttcattgcgt aaggttcaag ttcgttgtat agtttttcat ttccttcaac aaaaaccttc
     2041 aagcacatcg atcccacaac ttcagctgaa cctaaaatat attcttcgta tcccgattgg
     2101 tcgtaaacat tttggttcag gtccatctcc atacttttca aaaatgtatc gataagatgc
     2161 cggggcatgc gatagcggtt aactacttcc tggaaagcat taagaatggg gttcaatgaa
     2221 attttttctt caatggcttc gtaagtgtct ttcctgaatt tctccagaag cctggccttg
     2281 tcgtagtcat gaaaagtatc cacaatctca tccgcaaaac gaacaaatcc gtaaatggca
     2341 taaataggat catggaaacg tttgttcagg aagcggattc ccatggagaa agatgtactg
     2401 tatgcacgcg ttgtcatctt gctgcaacgg agcgatacgg tgtcgaaaaa agctttatcg
     2461 gccatgaatt agagtttggt tggaaggtgg aattgtttgg ctatttcttt tgctgctact
     2521 tgtccggaaa ttaatgttgg aggaacgccc ggaccgggaa ccgtgagctg acctgtgtaa
     2581 taaagattcg aaactttttt gcttttcatt tttggtttaa gaatcgcagt ctgacgcaag
     2641 gtgttggcga gtccgtaggc attgccttta aacgcgttgt aatcgccaat gaaatccttg
     2701 tgtgcataac tgcgctttaa aacaatgtgt tcgcggatct tctgattggt gaggtgttca
     2761 aaacgatcaa caaccagatt gaaatatttt tcgcggatct cttctgtgtc gttcaatccg
     2821 ggagcaaccg gaatgagaat gaaaaaggct tcgtgtcctt ctggggctat ggtggggtcg
     2881 gttaccgatg gacaagaagt ataaaataaa ggtttgctcg gccattgggg attgtcataa
     2941 atttctttgg cgtgctgacc aaaatcttca tcaaaaaaca atgtgtgatg aagcattccg
     3001 tttagttttt tattcagacc gagataaaaa atgagcgatg atggcgccaa cacgcgtttg
     3061 tcccaatagt tttcggtgta ttttctgctt tccttatcca gcaccttctg ctcaatatga
     3121 tgataatcgg cactggcaac aattacgtcg gctgtgagta tttcattgag tgtgtgtacc
     3181 gcaacaattt ttccgttttt ggtttcgagt cgtgttacag gtgtgttcag acgtacatca
     3241 acaccttttt ctttggcaag cgaggtcatt ccttccacaa tcttcatcat tccgcctttt
     3301 ggataccagg tgccgagttg catgtcggca taattcatca ggctgtacaa tgcaggagtg
     3361 ttttccggtt ttgcaccgag aaagaggact ggaaattcta aaagttgaag cagtcgtgga
     3421 tgcgaaaagt atttccggat ataggaggcg aatgatttga gcaaatgcat cttgaacaat
     3481 ccgcccatta cacgcttgtc gaggaattcg gtgatggata atccgggacg gtaaacaaga
     3541 tcatttactc cgacatcata tttgtatcca ccgtcagcca gaaatttttt gagtttttct
     3601 cccgcacctt tttcgtacga ttcaaacatc gcttccagtt ctttcatgtc tgatggaaca
     3661 tccataaaat cgttgggcgc aaaataaatt ctgtaggaag gagataagcg ttccagttcg
     3721 taataatctg atggtttctt atcgaacagc gcgaagtatt tttcgaatac atcaggcatc
     3781 cagtaccagc tcggacccat atcaaaactg aatccatcct tgcggtaaac acgtgcacgt
     3841 ccgcctgtct gttcgttttt ctccaaaagg gtgactttca tccccagctt ggccaggcaa
     3901 cttgcggtgg aaagtccggc gaaaccggct cctataacta tcgctgtttt ttgcatctta
     3961 attgtagcgg tagtctttaa attttttcat aaggatctgc cgtgcaagaa aaatacgact
     4021 tttcactgtg ccaattggta cacctacttc gtctgcgatg tctttgtact tatgtccttc
     4081 gagatgcatg ttaaaaggaa tgcggtattc gtcggacaat tcactgatcc ctttaagaag
     4141 ttcattttcc gaataggttg attcaggcga aatgcttcct ttcgtttgag ggatgtttac
     4201 aaaatgcaag tcttttgttc cgtcgatgat ggtattcgct tttttgcggc ggcggtagtt
     4261 gttgatgaag gtgttcttca ttatggtaaa caaccaggcc ccaagattgg tgctatccgt
     4321 aaatttttca cggtacgtaa atgctttcag aatagtatcc tgaagcaaat cctttgcatc
     4381 ttcctgatcg gaggtgagac taacggcaaa agccttcagt cggggacgca tcataataac
     4441 atgctgattg aattcgatgg ctgtcatggc gtttagtttt gttgaacaaa tctacgtaaa
     4501 cattaaacaa accaccaagt ttttgttgaa ctattttgtt aaaaagcttt aacaaaacaa
     4561 gataaataat tgattatcaa tcattggtgt atatacaaat tttcagaaac ggacgtcatt
     4621 taagggtaaa cggccctgaa gcgaaatcga ctgcacggag atttcttctt caggttcaat
     4681 tttccgttca ccgttcaccg gcctgtcccg accgtaccga aggggagcgt cgggatcacc
     4741 attcaccaaa aaggcagagg gaccgaacca aatccgcgcg cgcaggatat tgatccgatc
     4801 ccccctcgga aggactaaag aaaaatttag cgcaccaccc agcggtgggt ttcgctttgt
     4861 tcacccgtct gtatacggat gaggtaaatt cccggagcta aagtggatcc gttaaaggat
     4921 gttctcattc ccgaaaattg ttcgttgtgt atgatcttgc cctgaagatc acttatggtt
     4981 acctgatgca atgcatctgt atcgctttca ataaagagtt gctcttttcc cggattggga
     5041 taaattcgtg tatgttccaa ttgaacaatt tcggtttcgg gttgacgttc ctccaatgga
     5101 atcgcagcat caacgattac ctcttcaact ttttccagaa tatccggagc aacaatttcc
     5161 tccggaagat tttcatgtac aagaactgta tcccccataa ttacgttgca cgacacaaat
     5221 gttgaatcaa cttttgtaat taatgattcc tcaactactg tggtgtattc tgcatgtgga
     5281 atagtatcga tgttttcctg tggctcaacc catgcaactt ctcctaataa tgcatattct
     5341 tcttccagaa caattccaac acgagtactc gccttattga tgtacacctg ttccaggtat
     5401 ttgtatctga aatcgtgtga tgcacttata ctaatggagt tgttttttat ttttccggca
     5461 gcaatttcaa ttcggaagga accgttagcc atggttttta tttctccgat ttgttcatct
     5521 ccgctattga taataacagt cgcttgtgca acggctttcc catcgggagt tgtaacctgt
     5581 ccgtgaatga tacttggttg tgtatttgcg ttgtgtgttt caatgggcat aacatctccc
     5641 ttgatcgcca cttttcccat tttttgtgct ttggcttctt tgccgagatt gaataatccg
     5701 agaaaagcca atgaggcagc aagccatttt tttacacgaa attctcttgc agtagccggt
     5761 ttagctttca tgtgtgattc gcttagctgt gatggaagga atacgccaca cgtcttccct
     5821 tcggcttgtt ccaaatgctg cagaatgtcc ttttcagtct ttcgcgtgaa atcgaccact
     5881 tcttttgtgc aaatggcgca gaaacgtcct ttttcggttg gactcatagc cgcccagttt
     5941 tcgtgacagg gctccggaat gtagatggaa gacatgcttt ttcgtgttgg ttcaggagtc
     6001 tgtacacgcc aagcaggggg aggttcaacg gcaac
//
LOCUS       DAOMYG010000166         6018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4476_length_6018_cov_10.645145, whole genome shotgun sequence.
ACCESSION   DAOMYG010000166 DAOMYG010000000
VERSION     DAOMYG010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4476_length_6018_cov_10.645145"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PK610_06730"
     CDS             complement(<1..361)
                     /locus_tag="PK610_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRJ35663.1"
                     /translation="MLRRLNCLLWIVLWAIPISGLAQDPAFFRISSEQGLPSNEVYTV
                     HQDKKGFIWIGSDAGLFRYDGFRFKRYKSSGQKSRALSGLTESNDGSIYAYSFSGQIF
                     HTQGDSLVELPDWRKPLN"
     gene            397..1029
                     /locus_tag="PK610_06735"
     CDS             397..1029
                     /locus_tag="PK610_06735"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HRJ35664.1"
                     /translation="MEPYIRNVNSVLPIAYLPPVSYFAEYTRHANVLIESMEHFPKQT
                     LRNRCTILGANGPITLIIPVEKGRSGKTPVTDIRIAHRDNWQRNHWRTILSAYKNAPF
                     FEYYEHLFSPVFEKKHDWLMEFDLELLQVLDKCLNLKKNLQLSSVYIESYPSDFTDFR
                     SKSFNENDTESFPEYTQVFSDRHPFIPNLSVIDLLFNSGPDSVNYLMHLP"
     gene            1174..1557
                     /locus_tag="PK610_06740"
     CDS             1174..1557
                     /locus_tag="PK610_06740"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRJ35665.1"
                     /translation="MLLFLPQIMPELTAIHIRIRSLREARNISQDQIAERLGISSRSY
                     ARLESNDVDLTVERLIRIAEIMDIKPIEILDPDQDPRSVLRDMELLQKEFMNQVVLPS
                     KKREDLLKKLEILLEILNSQSQVLG"
     gene            1615..2874
                     /locus_tag="PK610_06745"
     CDS             1615..2874
                     /locus_tag="PK610_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRJ35666.1"
                     /translation="MDASRRKFLSTTAKAAGGLGLLSVSDLSFSQIAPPLNTSEDDFW
                     SWVRHSYTVSQNIVNLNNGGVSPQPKVVQEAFEHYNRFSNEVPTYYMWRVLDKGREDV
                     RRDLAKLAGADPDEISINRNATEALDIIIYGIDLKKGDEVIVTNYDYPNMRHAWMQRE
                     QREGIKLVWIDIPVGLDNEEAVVKLFTDAISTKTKVIHLTQVINWTGHILPAKRICEE
                     AKKKNILTVMDAAHSFAHYEMNLHDIGCDFAGTSLHKWLCAPFGTGMLYVKKERIANL
                     WPIMSSENPKSDDIRKFEALGTRSFPAEMGIGRAISFHESIGSKRKEERLRWLKNYWV
                     NKVKQHPKIKFYSPLSDHASCAIATVGIEGKKATEIESYLFDKFSVHTVAIEWQKVNG
                     IRVTPHVYTSTSDLDKLVKGLLELADR"
     gene            2984..3562
                     /locus_tag="PK610_06750"
     CDS             2984..3562
                     /locus_tag="PK610_06750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ35667.1"
                     /translation="MNGMQNNNTKYSREEELLQINAAKADPARFAVLYDHYYKPIFLF
                     IHHRIRDKQLSGDLTAQVFLKAMINLGKYRDQGHPFSSWLYRIASNEVNMYFRQSKRE
                     SDIEVKESDAVEMMEEINELYSEENCTLILEALAELPEELNVLIELRFMQKLSFLEIA
                     GMYNITEANAKMKVYRALEKLKQKFNERRGRK"
     gene            3559..3765
                     /locus_tag="PK610_06755"
     CDS             3559..3765
                     /locus_tag="PK610_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35668.1"
                     /translation="MKKYKLHKENSGQQISDEEMMKYKDFSRLKHQYDTVTKRPKKPL
                     YKNPYMFFVLVLIILLALLLAGEL"
     gene            complement(3777..4379)
                     /locus_tag="PK610_06760"
     CDS             complement(3777..4379)
                     /locus_tag="PK610_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35669.1"
                     /translation="MKRVLFFSAVVLLLCSSFSWFQSRDAVVESKSGLGVFIMCEPVQ
                     AYDKLFVVKTAHKSSKYVGGLPVGVVLGIDDPIDRLVQRALRKGRKKNIDAIITSDAR
                     MAVAIKFRGAPTVDERLRARPVSISGVDVYVKSKPQSSYLEVATISLKTENRLTVPGI
                     DTDRLAKMIQTLIEQGKKAGYTFQGLITSDGVSATLISYK"
     gene            complement(4461..4877)
                     /gene="tnpA"
                     /locus_tag="PK610_06765"
     CDS             complement(4461..4877)
                     /gene="tnpA"
                     /locus_tag="PK610_06765"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HRJ35670.1"
                     /translation="MIKTSSKTLIYIHVVFSTKHRSPTIRPEVEGAVYSIIRAQCIER
                     KISLIALGGMPDHVHMLLVLPCHLPLSEVVGRIKGASSKAVNDAGLVPGGFAWQVGYG
                     AFSVSEQGVNAVRRYIENQKEHHSTKGPLSDWELPD"
     gene            5069..6004
                     /gene="dinB"
                     /locus_tag="PK610_06770"
     CDS             5069..6004
                     /gene="dinB"
                     /locus_tag="PK610_06770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRJ35671.1"
                     /translation="MERNIVHMDLDSFFVSVECQLNPKLIGKPVIIGGTSDRGVVSSC
                     SYEARRFGVHSAMPIRMAKKLCPDALIVRGDYESYTKYSGMVTDIVRENVPVYEKSSI
                     DEFYVDLSGMERFFSSYKLATELRQKIIRETGLPISFGLSQNKTVSKVATGEAKPNGQ
                     QKVDRGTEKPFLAPLSIKKIPMVGEKTYTLLRSMGVEKVHTLQQMPPEMLHTALGEHG
                     RMIWERANGIDESPIIPYSERKSISTETTFEEDTIDVEMLNYKLVSMCEELAFSLRKE
                     EKLCSCITVKIRYSNFDTHTKPSPSINFPNVATQP"
BASE COUNT         1841 a         1414 c         1216 g         1547 t
ORIGIN      
        1 tattaagtgg ctttctccaa tcaggcaatt caaccagaga atcgccttgg gtatgaaaaa
       61 tctggccgga aaaagaatat gcataaatac ttccatcatt tgattctgtc aatccggaaa
      121 gcgccctcga tttttgtccg gaagacttat accgtttaaa gcgaaaccca tcatacctga
      181 ataaacctgc atcgctgcca atccagataa atcctttttt atcctgatgc accgtgtaaa
      241 cttcattact cggaaggcct tgttcggacg agatccgaaa aaatgcagga tcctgcgcta
      301 aaccggatat tggtatagcc cacagaacaa tccacaacaa acaattcaat cgcctcagca
      361 cagaacgaaa ataggaattc aaacagagtc tgcgatatgg aaccttatat tcgcaatgtg
      421 aattctgtgt tacccatcgc ctatcttccc cctgtctcct attttgcgga gtacacacgg
      481 catgcgaacg tgctgatcga gagcatggag cactttccga aacaaacctt gcgaaaccga
      541 tgtactattc tgggagcaaa tggtccgatt acacttatta tacccgttga aaaaggcaga
      601 agtggcaaaa cgccggtgac ggatatccgc attgcccatc gcgacaattg gcagcgtaac
      661 cattggcgga ccatcctctc agcgtataaa aacgccccct tttttgagta ttacgaacat
      721 ttattttctc ctgtattcga aaaaaaacac gattggctta tggagtttga tctcgaactg
      781 cttcaggttc tcgacaaatg tttgaatctc aaaaaaaacc ttcaactaag ctcagtatac
      841 attgaatcct atccatctga ttttacagat ttcagaagca aatcattcaa tgaaaacgat
      901 acggaatcct ttcctgaata tactcaagtg ttttcagaca gacatccatt tatccccaac
      961 ctttcggtta tcgatctgct tttcaatagt ggtccggatt ctgtaaacta tctcatgcat
     1021 ttaccgtagt aatttttcac tttaacatca ataattgtta attaaaacat tgttttcgaa
     1081 tgcaattatt tgctttaaat tattgattta tcattcatta gaattttaat aatcccctct
     1141 atttttagct aaaatcatcc tgacaattcg ctaatgctgc tatttttacc gcagattatg
     1201 ccggaactta cagctattca tatccgcatt cgctctttgc gtgaagcaag aaatatttcg
     1261 caggaccaaa ttgccgagcg acttggcatc tcttcccgaa gctatgcaag acttgaaagt
     1321 aatgatgtgg atcttacggt agaacgactt attcgcatcg ctgagattat ggacatcaaa
     1381 cccatcgaaa tactcgaccc ggatcaggat ccaagaagtg tattaagaga catggaattg
     1441 ttgcaaaaag aattcatgaa ccaagtcgtt ctcccttcaa aaaagagaga agacctgctc
     1501 aaaaaacttg aaatcctgct cgaaatcctg aattcacaaa gtcaggtgct cggttagtaa
     1561 attctccctt ccattattcc ccattatcgg ttatattgca ggaaagctat tactatggat
     1621 gcatcccgca ggaaattttt atcgactaca gccaaagctg ccggtggact tggattgttg
     1681 tcggtaagcg atctatcatt ttcgcagatt gctccaccac tcaatacaag tgaagacgat
     1741 ttttggtcgt gggtgcgtca ctcctacact gtatctcaaa acatcgttaa tctcaataat
     1801 ggcggagtga gtcctcagcc caaagtcgtt caggaagctt ttgaacatta caatcgtttt
     1861 tcgaacgaag ttcctactta ttacatgtgg agggttctgg ataagggaag agaagatgtg
     1921 cgtcgcgacc ttgcaaaact tgccggtgct gatcccgatg aaatttcaat aaaccgcaac
     1981 gccaccgaag cgctggacat tatcatttac ggaattgatc tgaaaaaagg agatgaggta
     2041 atcgtaacca attacgacta tcccaacatg cgtcacgcct ggatgcaaag ggaacaacgc
     2101 gaaggcatca aactcgtgtg gatcgatatt cctgttggtc tcgacaatga ggaagcagtc
     2161 gtgaaattat ttaccgatgc gatcagtaca aaaacaaaag tgatacactt aacacaagtc
     2221 ataaactgga caggtcatat tttacctgca aaacgcattt gtgaagaagc aaagaaaaaa
     2281 aacatcttaa cggtaatgga tgccgcccat tcttttgcgc attacgaaat gaatctgcac
     2341 gatattggtt gtgactttgc aggaacatca ttgcataaat ggttatgcgc accctttggt
     2401 acaggaatgt tgtatgtgaa aaaagaacgt attgcaaacc tgtggccgat aatgtcgagc
     2461 gaaaatccga aaagcgatga catccgcaaa ttcgaagcct taggcacacg ttctttccct
     2521 gcggaaatgg gaattggcag agcaattagt tttcatgaat ccatcggaag taaacgcaag
     2581 gaagaacggc ttcgctggct gaaaaactac tgggtgaaca aggttaaaca acatcctaaa
     2641 atcaaattct attctccgct ttccgatcat gcctcgtgtg ctattgcaac ggttggaatt
     2701 gaaggtaaaa aggcaaccga gatcgaatcc tatttatttg ataagttcag cgtacataca
     2761 gtagccatcg aatggcagaa agtaaacgga atcagagtaa caccacatgt ctacacttca
     2821 acaagtgatc ttgacaaact ggtaaaggga ttgctcgaat tagccgatcg ctaagttctt
     2881 ctgccggaaa ctggtcagcg aacaccgcaa ctgattaatt taaagaggat taaaaaaacc
     2941 tgttactttt tcatgaaagc acattgtagt attagtgatg tgaatgaacg gcatgcaaaa
     3001 caacaacacc aaatacagca gggaggaaga gcttttgcag atcaatgctg caaaggccga
     3061 tcccgcccgc tttgcggtgt tgtatgatca ctattacaaa cccatttttc tctttatcca
     3121 tcaccgcatc cgcgacaaac aacttagtgg cgatctgact gcacaggttt ttctgaaggc
     3181 gatgattaac cttggcaaat accgcgacca aggccacccc ttttcttcgt ggctctaccg
     3241 cattgcatcg aatgaagtaa acatgtactt ccggcaatcg aaacgcgaat cggatatcga
     3301 agtaaaagaa tcagatgctg ttgagatgat ggaagaaata aacgaacttt actccgaaga
     3361 aaactgcacc ctgattctgg aagcgcttgc agaacttccg gaggaattaa atgtgctgat
     3421 tgagcttcgg tttatgcaaa aactttcgtt cctggaaatt gcaggaatgt ataacataac
     3481 agaagcaaat gccaagatga aagtgtacag ggcacttgaa aaactgaaac aaaaattcaa
     3541 cgaacgccgg gggcggaaat gaaaaaatat aaactacata aagaaaatag tggtcagcag
     3601 atctctgatg aagagatgat gaagtacaag gatttttcgc gtttgaaaca ccaatacgat
     3661 acagttacca agcgtccgaa aaaaccactt tacaaaaacc cttatatgtt ttttgtactg
     3721 gtactgatta tcctcctggc cttattactt gcaggagaat tgtaagcaca ttccatctac
     3781 ttgtaagaaa tcagcgttgc acttacccca tcacttgtga tcaatccctg aaaagtatat
     3841 cctgctttct ttccctgttc gatcaaggtc tgaatcatct ttgccagtct gtcggtatca
     3901 attcccggca cagttagtct gttctccgtc tttaaggaaa tggtagctac ttcgaggtac
     3961 gacgattgtg gtttgctttt cacatacaca tccactccgc ttatacttac cggacgcgca
     4021 cggagtcgtt catccactgt tggcgctcct ctgaatttta tcgctactgc catccgtgca
     4081 tcagaggtaa tgattgcgtc aatatttttt ttccgtccct tacgtagagc gcgttgcacc
     4141 aggcgatcga tgggatcatc tattcccaac accactccca ccggaagtcc gcctacatac
     4201 ttggacgatt tatgcgcggt cttaacaaca aaaagtttat cgtacgcctg aaccggttcg
     4261 cacatgataa atacccctaa tccggacttg gattccacca ccgcatcgcg cgactgaaac
     4321 caggaaaagg aggaacataa cagtaaaaca actgcagaga aaaataaaac acgtttcata
     4381 gctcaatgat ttggaaatgg aatattgcaa gaatagttcc aaaacacaag aacaagataa
     4441 tgactttcct cagcagcgca tcaatccggc aattcccaat cggacaatgg tccctttgtg
     4501 gaatgatgtt ccttttggtt ttcgatatac cttctaaccg cattcactcc ctgctccgac
     4561 acagagaatg cgccatatcc cacctgccac gcaaatccac ccggaacgag accggcatcg
     4621 tttactgctt tggaacttgc gcctttaatc cttccaacca cttcagagag tggcagatga
     4681 cacggtagca ccaacaacat atgcacatga tccggcatgc ccccgagggc aatcaatgag
     4741 atctttcgct caatgcattg tgcacggatg atcgaatata ccgccccttc aacctccggg
     4801 cgaatcgtag gactacggtg cttcgtggaa aacacgacat gtatgtaaat caatgtcttt
     4861 gacgatgtct taatcatgta actgtcttat tatgaaacga aattaatcat aatgacgaat
     4921 ttttcatcat ttaatgatga aaaattcgtc ggtatatcta tatttggaac ggggttcccg
     4981 gatatgtaac cgggggctta agcccccggc tacatatcgg atgcccccgg ctacatatcg
     5041 gatgccacgg actgaatgac atacaactat ggaacgcaac atcgtacata tggatctcga
     5101 ctccttcttc gtatcggtcg agtgtcagct caacccgaag ctcatcggca aaccggtcat
     5161 cattggcggc acgagcgaca ggggtgtggt ctcttcatgc agctacgaag cgcgtcgttt
     5221 cggtgtgcac tcagcaatgc ctatacgtat ggcgaaaaaa ctgtgtcccg acgctctcat
     5281 cgttcgtggc gactacgaga gctacaccaa atattcggga atggtaaccg atatcgtacg
     5341 cgagaatgtt cctgtatatg aaaaatcatc gatcgatgaa ttctatgtcg acctcagcgg
     5401 aatggagcgc ttcttcagca gctacaaact ggcaacggaa ctgcgtcaga aaattattcg
     5461 tgaaacagga ctacccatct cctttgggtt atcgcaaaac aaaaccgtat cgaaagtcgc
     5521 caccggagaa gcaaaaccaa acggtcagca aaaagtagat cgcggaacag aaaaaccttt
     5581 tctcgctcca ttatccatca aaaaaattcc gatggtgggc gaaaaaacct acaccctgtt
     5641 gcgctcaatg ggagtggaaa aagtacacac cctgcagcag atgccacccg aaatgctgca
     5701 tactgcattg ggcgaacacg gacgcatgat ctgggaacgc gcgaacggca tcgacgagag
     5761 tccgataatc ccctactccg aacgcaaatc gatctctacc gaaacaacat tcgaagaaga
     5821 caccatcgat gtggaaatgc tcaactacaa acttgtaagc atgtgcgaag agctcgcttt
     5881 ctccttacga aaagaagaaa aactctgctc ctgcatcacc gtaaaaatcc gctattcaaa
     5941 cttcgacacg cacaccaaac cgtccccgtc aatcaacttc cccaacgtag caacccagcc
     6001 ctgatttcga agggatta
//
LOCUS       DAOMYG010000167         5998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4502_length_5998_cov_7.872960, whole genome shotgun sequence.
ACCESSION   DAOMYG010000167 DAOMYG010000000
VERSION     DAOMYG010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4502_length_5998_cov_7.872960"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PK610_06775"
     CDS             complement(<1..183)
                     /locus_tag="PK610_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35672.1"
                     /translation="MVKAKRYKAAILPYVLVITLAGGIVVSSIMLRFYLSLKESDLLL
                     NQWIIEKDLNSVLNYGM"
     gene            complement(194..856)
                     /locus_tag="PK610_06780"
     CDS             complement(194..856)
                     /locus_tag="PK610_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35673.1"
                     /translation="MKNWNHLLLIMFLITPTVLRAQEENLRLFQINTNGEHIKAEAYV
                     KPQRIKVNNHLYYYWIKAREIHFSQGGYEGNLLHGSYVSFYPSEQLKEQGRFYKGLKN
                     GEWKKWHPNGAVAEISNWHKGMLNGEVLRFNEAGRRIYKAHYKGNVLHGRELVFVQDT
                     ISSQRVYKRGVEKVKKVKAEKKRKFKKSGIQVMEEKTQEPENADPKDRGRKASKKKSS
                     GN"
     gene            complement(853..1365)
                     /locus_tag="PK610_06785"
     CDS             complement(853..1365)
                     /locus_tag="PK610_06785"
                     /inference="COORDINATES: protein motif:HMM:NF022800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HRJ35674.1"
                     /translation="MNKKLLNIVLIVVVCGIWSVVIYRFIKNQSSQEMVIDIENQAGP
                     VSDFSTKREDFPLSSISRDPFLDGTTPLRKINKGGRNLVSGTSVSTNSGKEKIQKTVH
                     VADVAPMNLRFQGVVKNNTTNATVALIAFNGKSVRLCPGQIHEGYKLEKIYRDSVMLT
                     RNKERIIARK"
     gene            complement(1365..2309)
                     /locus_tag="PK610_06790"
     CDS             complement(1365..2309)
                     /locus_tag="PK610_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35675.1"
                     /translation="MASLLLILSGCQDFFVRDLTGDTVVLSSPANGTQTVALSHQFTW
                     EEIKGAEYYNLEIVTPDFGAIQSYVLDTTISSTRFSQTLFPGTYQWRVKAGNFGGETQ
                     FSQPFTLIIDTSSDLSNQVVVLNSPFSGFVTNDSTPSFSWQTIFIADSYVFTLKSGTV
                     WNSGIVIYSDTLLATSMALPAINALPEGKYTWGVKAMNAISSSSYTIRNLHIDRTDPP
                     AVSLQSPSNFSLLSTGILNFNWNRPADVGTQPSNRADSVYVYSDTTLTNLFSRSGTST
                     TSIALTLTNPGWYRWYVISFDQAGNASDTSQKFKFQLN"
     gene            complement(2302..2832)
                     /locus_tag="PK610_06795"
     CDS             complement(2302..2832)
                     /locus_tag="PK610_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35676.1"
                     /translation="MGKLNYKRKFLAVAVLVPVLALLSWFRLIDPTLELKGQCRSMEQ
                     SLDSSKGYETDYLLLRNELDQLNQMLGKEAGSVEEINRGIVEFVSKNSDELNISLTEF
                     PAVHSFQKNQYRIFTHQAEIEGSYMAILRLVYKMEREFNLARICSIRFVTRQDKIKKV
                     KKLYGIIYVQNIHRLG"
     gene            complement(2832..4028)
                     /locus_tag="PK610_06800"
     CDS             complement(2832..4028)
                     /locus_tag="PK610_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35677.1"
                     /translation="MLPFLQHIADSKEKKLNSIELAFMADGMVKVLYPVQFLMRKKGV
                     EFAGLEEVSNLPSEWKKTLNKEIALLITFSGKGIITKRIEKGEGYNFHIRTILPNANP
                     EEFYWQVIDCRDHQMVSVIRKTQIDDFLKEVCASNYLIAGVFLGAINAFPLAGITGSP
                     EISASGFRIEMEANEPSAIVPDQNFSEELFVDGKKILPTQLVAVGSGFGYYSDVPGIE
                     TSYHTKEELGYRNLLNKIGFGALAVLMIILGFNYYFRNYYQERFLLLSEIMTATAGER
                     QEMAGIQLELGEKRELIHSTGLSMHHWLSYYANDIGESVPLEIVLTDMIICPLQGKRM
                     EPGEPAEFLAGQILITGTTPGSEILHQWVERLKSRDWAREVIVESFKQKEDMDLSQFT
                     LQITLS"
     gene            complement(4029..5918)
                     /locus_tag="PK610_06805"
     CDS             complement(4029..5918)
                     /locus_tag="PK610_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35678.1"
                     /translation="MKHFLIVLTSLISMNCYSQDRFDKMRNKLEVQVVDMPGLSQSIQ
                     MNVNGVRIQDFLATIAENHKLNISVDPSLNLEVYYNFSDAQVLDVLIFLCRQYNLDIE
                     TTGTIMLFKKFTEPVPAIVPYSPRKPKVNYAKETNFLSLDLKRDSLEYVVQEITEQSM
                     YNIIVSPAAKGHQVSVFIANRPFENALELMCDANGLKLIQKGETVYLIDKGENQTSPG
                     TPVSKTFKAATVAVNGLDVKTNNKGTISVRAENVPLKDIIREVSNQMLINYFLFNEPT
                     GNTSLYIENAPYDDFLGYLLTGTSYTYKKQDSIYMIGDRKIEGLRKTQLIKLKYRPVE
                     KIMNFIPAELKKDVEIQEFIELNGLIVSGSSLRIDEIQQFIRQIDQLVPVVMIEVIIV
                     ESKRNHNVTTGVQAGLGTTNNTTPTNGGVSPGVNLEVSPSTINGIINGFNAAGVFNLG
                     NVSPRFYLSVQALEENGNIHVRSVPKISTLNGNLASMKVGSQEYYLEQTQNVYASNTT
                     QTIVNNQYKSVNADLTIKIKPFVSEDNQVTLTITVEQSDFTAKIAPTAPPGQVTRTFE
                     SMIRVRDGDMIVLGGLEEKTVENNASGLPGVARIPVLRSLFGKNSRKKSKSHLTVFIR
                     PTIVY"
BASE COUNT         1707 a         1304 c         1070 g         1917 t
ORIGIN      
        1 cattccgtaa ttcaatacag agttcaaatc cttttcgatg atccactgat ttaacagaag
       61 atctgattct ttcaatgaga ggtaaaaccg caacataatc gaagaaacaa caatgcctcc
      121 ggccaaagta atgaccagta cgtatggcaa tattgccgct ttgtatcgtt ttgccttaac
      181 caaaggattt tacttaatta ccgctgcttt tctttttcga tgctttacgt ccccgatctt
      241 tcggatctgc attctcaggc tcttgagttt tttcttccat cacctgaatt cccgatttct
      301 taaactttcg ctttttttcc gctttcacct tcttaacctt ttctactcct cttttataaa
      361 ccctctggga agaaatggta tcctgaacaa aaactaactc acgtccatgc aatacgtttc
      421 ccttgtaatg tgctttgtaa atgcgtcgtc ctgcttcatt aaaccgcagc acctctccgt
      481 ttaacattcc tttatgccaa ttactgattt ctgcaacagc accattggga tgccactttt
      541 tccattctcc gttcttgaga cctttataaa atcgtccctg ttcctttagt tgttcgcttg
      601 gataaaaact cacataactt ccatgcaata aattcccctc gtatcctcct tgcgaaaaat
      661 gaatttctct tgcttttatc caataataat acaagtgatt attcacttta attctctgcg
      721 gttttacata agcttctgct ttgatgtgtt cgccgtttgt attgatttga aaaagacgta
      781 aattttcctc ttgcgctctt aatacagtcg gtgtaatcaa aaacatgatc aacaacaagt
      841 gattccaatt tttcattttc gtgcaattat tcgttcttta ttgcgggtaa gcattaccga
      901 gtcgcggtaa attttttcaa gcttatatcc ctcatgtatt tgtccgggac acaatctcac
      961 actttttcca ttaaaggcaa tcagtgcaac tgtggcattg gtcgtattgt tttttaccac
     1021 tccctgaaat cgtaaattca ttggagcaac atcagctaca tgcacagttt tttggatctt
     1081 ttcttttcca ctatttgttg aaactgatgt tcctgatact aaattacgtc cccccttgtt
     1141 tatctttctt aaaggagttg ttccatcaag aaacggatca cgactaattg aactcaatgg
     1201 aaaatcttcc cgtttagttg aaaaatcaga aaccggtcct gcttgatttt ctatatcaat
     1261 taccatttcc tgagatgact gatttttaat gaaacgatat atcactaccg accagattcc
     1321 gcaaacaaca acaatcaata cgatatttag cagtttctta ttcattaatt caattgaaat
     1381 ttaaacttct gcgaagtatc cgatgcattt ccggcctggt cgaaactaat gacataccat
     1441 ctgtaccaac ccggattggt gagtgtaaga gcaattgaag ttgtactcgt tccacttctc
     1501 gaaaacaaat tggtcaatgt tgtgtctgaa taaacataca ctgaatcagc tctgttgctg
     1561 ggctgagttc caacatcagc cgggcgattc caattgaaat tcaaaatacc ggtggacagc
     1621 agtgaaaaat ttgaaggtga ctgcaatgaa actgcaggtg gatcagttcg gtcgatgtga
     1681 agattgcgaa tcgtgtaact actggaagag atcgcattca tagcctttac tccccaggta
     1741 tattttcctt ccggtaaagc attaattgca ggcaatgcca tagatgttgc cagcagggta
     1801 tcggaataga tgactatccc ggaattccaa actgttccag attttaaggt aaagacatag
     1861 ctgtcggcaa taaaaatagt ttgccatgag aatgacggcg tagaatcgtt ggtaacaaaa
     1921 cctgaaaacg gcgaattaag caccaccacc tgattgctga gatcactgct tgtatcaatt
     1981 atcaaagtga atggttgaga gaattgagtt tcaccaccaa aattccctgc cttcactcgc
     2041 cattgatacg taccgggaaa aagggtctgt gaaaatcggg tgcttgaaat ggtagtatcc
     2101 aacacgtagg attgtatggc gccaaaatca ggtgtcacaa tttcgagatt gtaatattct
     2161 gcacctttta tttcttccca ggtaaattga tgggataatg ccactgtttg cgttccgttt
     2221 gcaggagaac tcaatacaac cgtatctccg gtaagatccc tgacgaaaaa atcctgacat
     2281 cctgaaagaa tcaggagcaa actagccaag ccggtgaata ttctgaacat agatgatccc
     2341 ataaagtttt tttacttttt ttattttgtc ctgacgcgtt acaaagcgaa tggaacatat
     2401 tcgtgccagg ttaaattcgc gctccatttt atatacaagt cgtaaaattg ccatgtatga
     2461 accctcaatt tctgcctgat gcgtgaaaat ccggtattgg tttttctgaa agctgtgtac
     2521 agcaggaaat tcagtaagcg aaatgtttaa ttcatctgaa tttttactaa caaactccac
     2581 tattccccta ttgatttctt ctaccgaacc ggcttccttt cctaacattt gattcaattg
     2641 atccaattca ttgcgcaata acaaataatc tgtttcatat cccttactcg aatccagaga
     2701 ttgttccatg ctcctgcact gccctttcaa ctcaagtgta ggatcaatca atctgaacca
     2761 ggataacaat gccaatacag gcaccaacac tgccaccgcc agaaatttcc gtttgtaatt
     2821 taattttccc atcaggaaag cgttatttgt aaggtaaact gcgacaaatc catatcttct
     2881 ttttgcttaa aagattcaac tattacttct cgtgcccagt ctctcgattt taagcgctcc
     2941 acccattgat ggagaatctc tgaacctggc gtggttccgg taattagaat ctgacccgcc
     3001 aaaaattctg caggttcacc gggttccatt cgctttccct gtaatggaca aataatcata
     3061 tctgtaagca caatttcaag aggaacactt tcaccaatat cgtttgcgta atacgacaac
     3121 cagtgatgca tgctgagtcc ggttgaatga atcaattccc tcttttcacc cagttccaat
     3181 tgtattcccg ccatttcctg acgctcaccg gcagtggcag tcatgatttc acttaacagc
     3241 aaaaaccgct cctgataata attccggaaa taataattaa acccaagtat gatcattaac
     3301 accgctaatg caccaaatcc aattttattc aatagatttc tgtaccccag ttcttctttg
     3361 gtatggtagg aagtttcaat tccgggaaca tctgaataat acccaaatcc ggaacctact
     3421 gcaactaact gagtggggag aatttttttc ccatcaacaa atagttcttc ggaaaaattc
     3481 tgatcaggaa cgattgctga tggttcgttc gcttccattt caatgcgaaa tccggaagca
     3541 gaaatttccg gagagccagt tattcctgcc aatggaaaag catttattgc tcccagaaaa
     3601 acgccggcaa tcaaataatt ggatgcacat acttctttga gaaaatcatc gatctgagtt
     3661 ttgcgaatca cacttaccat ttgatgatca cgacaatcaa tgacctgcca atagaactct
     3721 tcaggattag cattgggtaa aatagttcgg atgtgaaaat tgtatccctc acccttttcg
     3781 attcgttttg taataattcc tttgccggaa aatgtaatca gcaatgcaat ttccttattc
     3841 aaggtcttct tccattctga tggaagattt gaaacttctt ccagtccagc aaactcaacg
     3901 ccttttttgc gcatcagaaa ctgcacggga taaagtacct tgaccatccc atcagccata
     3961 aacgcaagct caattgaatt cagcttcttt tctttcgaat cagcgatatg ttgtaaaaat
     4021 ggaagcactt aatacactat ggttggacga ataaatacag taagatgtga tttgcttttc
     4081 tttcgggaat tctttccaaa aagagagcgt aataccggaa tacgggcaac cccgggcaaa
     4141 cctgaagcat tgttttcaac tgtcttttct tcgagtccgc ccaaaacaat catatctcca
     4201 tcgcgtaccc tgatcatgga ttcgaatgtt cgcgttacct gaccgggtgg tgcagtaggt
     4261 gcaattttcg ctgtgaaatc agattgctca acagtaatgg ttaaggtgac ctgattatcc
     4321 tccgatacaa atggtttaat ttttatcgtg agatctgcat ttacagattt gtattgattg
     4381 tttacaatag tttgagtggt attggatgca taaacattct gagtttgttc gaggtaatat
     4441 tcctggcttc cgactttcat cgatgccaga ttaccgttta gtgttgaaat tttagggaca
     4501 ctacgcacat ggatgtttcc attttcctca agcgcctgaa cagagaggta aaaacgcggg
     4561 ctcacgttgc ccagattaaa tacacctgcg gcattaaatc cgttgatgat tccattaatg
     4621 gttgatggag aaacttccag gttaacgccc ggtgaaactc caccattggt tggtgtagta
     4681 ttattagttg ttcccaatcc agcttgtaca cctgtagtta cattatgatt gcgctttgat
     4741 tcgacaatga taacctcgat cattaccaca ggaaccaact gatcaatttg tcggatgaat
     4801 tgctgtatct catctatacg caaagaagat ccacttacaa ttaaaccgtt gagttcaata
     4861 aactcctgta tctctacatc ctttttaagt tcagcaggta taaaattcat gattttctca
     4921 acgggcctgt acttcaattt tatcaattgc gtttttcgaa gtccttcaat ttttcggtcg
     4981 cctatcatat atatgctgtc ctgcttcttg taggtatagg atgttcccgt aaggagataa
     5041 ccaagaaaat cgtcgtaggg tgcattctcg atatacagag aagtattccc tgttggttca
     5101 ttgaataaaa aataattgat cagcatctga tttgatactt ccctgataat atcttttaaa
     5161 ggtacattct cagctctaac actgattgtt cccttgttat tcgtttttac atcaagtccg
     5221 tttactgcga cagtggctgc tttgaatgtt ttgctcactg gtgttccggg gcttgtttga
     5281 ttttcgccct tgtcgatcag gtaaactgtt tcaccttttt ggattagttt aagcccattg
     5341 gcatcacaca taagttccaa tgcattttca aacggacggt ttgcaataaa aacactgacc
     5401 tgatgtccct tcgcagccgg agatacaatg atgttgtaca tactttgttc tgttatttcc
     5461 tgtaccacat attctaagct gtctcgcttt aaatcaaggg aaagaaaatt tgtctctttg
     5521 gcataattta ccttaggttt tctgggtgaa tatggaacaa tagcaggaac aggttcagta
     5581 aactttttga aaagcatgat tgttcctgtt gtttcaatat ccagattata ttgacggcat
     5641 aaaaaaatca gtacatccaa cacctgagca tcggaaaaat tatagtacac ttccaaattg
     5701 agtgaaggat ctacactgat attcagtttg tgattctccg caatagtagc caggaaatcc
     5761 tgaatccgaa caccgttcac attcatctga atgctttgcg ataagccggg catatcaact
     5821 acctgtactt cgagtttatt tcgcatttta tcaaacctat cctgcgaata gcaattcatc
     5881 gaaatcaggg aggtcaatac gatcagaaaa tgtttcattt tattgtgtat tcataagtaa
     5941 tggataaact tcctcaagtg acgtctctcc ggatttaaaa agctggaatg cattctca
//
LOCUS       DAOMYG010000168         5972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4534_length_5972_cov_8.341220, whole genome shotgun sequence.
ACCESSION   DAOMYG010000168 DAOMYG010000000
VERSION     DAOMYG010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4534_length_5972_cov_8.341220"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PK610_06810"
     CDS             <1..389
                     /locus_tag="PK610_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRJ35679.1"
                     /translation="KEFEMQQSVKALIRGGFINAAHDISDGGLWVTLAEMGMPRGLGF
                     DIESDSELRLDAFLFGESQGRVVVTVSEEAQAEFIEFMATQETPFTLLGHVTKGKVVV
                     DGEHYGFVHEVKPVYDNSLGEKIMSN"
     gene            391..861
                     /locus_tag="PK610_06815"
     CDS             391..861
                     /locus_tag="PK610_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35680.1"
                     /translation="MARTRRTPEEQDAIRAMVAEKQTEMIAAKGDKAVREGRIIIIIL
                     SVLVAIAAVAEYFMYGEDPIILAVYAPFILAFVAFAIFYYRNPFALSIAALSVYGFMQ
                     IAGALADPTNLAKGIIIKVIIIAGLIKAIKSARDFRVRNKNEQKTGSDILDQDF"
     gene            complement(865..2844)
                     /locus_tag="PK610_06820"
     CDS             complement(865..2844)
                     /locus_tag="PK610_06820"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRJ35681.1"
                     /translation="MIRLLVITLFCCIGTLRAQDRMEIVNRAKEDLKQAKSDSARIFF
                     LNKISWNISYSDLDSGLYYGNLALELAQSTNDRSQLGIVYTTLATIHQDKGNLSIAAE
                     LYEKSIKAHEAINDKSGLATTLSNYSNLEGLRGNRKKAKELLFTALGYFKLENNKRSI
                     ALVTSNLGTHYLEENKLDSAEYFLEIGNSYCKEYAITDLLSSNTAVLARVHSKQGKKK
                     QAIEEIEFALELLSIRKSPYDLVQVEQIYADMLTDYQDYYQAEKYYLSAREKAEMVGM
                     KDHIMKINLSLSGLYEKMGLLHKSLAEYKNYAALKDELNEASALEKLNHMSYRMENER
                     KSKEIELLRQKSRIQDLDQDRQHEQIRTQNIIIIGGALLFLLVIVMSIHLFRSNKRKQ
                     QTNALLTEQNHIIEHKNKEITDSITYARRIQDAILPSKDVLNTIFPEHFILYLPKDIV
                     AGDFYWVLEHKGKIFFAVCDCTGHGVPGAMVSVVCHNALQSVVREMNITDPGEILNHV
                     RTLVTETFQSKQSEVRDGMDVSLGVLDKSKAVLTWAGANNPLWIVRSNTAAIQEIKAH
                     KQAIGKTDDPTPFPSHTIQLEKGDLIYLFSDGFADQFGGKDGKKFKSSGLKELLRSVA
                     VNPIALQHQLLHEAHLQWKGDYEQVDDICFFGVRV"
     gene            2914..4791
                     /locus_tag="PK610_06825"
     CDS             2914..4791
                     /locus_tag="PK610_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007093835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRJ35682.1"
                     /translation="MNYLSVEELSKNYGEKNLFSGISFGIDQGQRVALVAKNGAGKST
                     LMKILAGKEVADSGKAVFRKDLRIGFLEQDKQFTPGTTVIESALDSDDPAALAVKNYT
                     AATHSGNEEQLQQAMDSMTATNAWDYETRVAQILGKLGLHDATQLVDSLSGGQTKRLA
                     LAQVLIRQPEFMILDEPTNHLDLDMIEWLEVFLNGSEITLFMVTHDRYFLERVCNEIV
                     ELDGGQLYRYKGNYSYFLEKKAEREMNEQSEISKAKNLYRKELDWMRRQPKARGTKAR
                     ARIDAFHDTESIASKRIKKDEIEFNVNMQRIGGKILEFHKVKKQFGERIILQGFDYLF
                     KRGEKIGIVGPNGVGKTTFLKMIMGQEEPDGGKITLGETVAIGYYSQEGMKLNEDKRV
                     IEVIREIAEFIPLAGGKKMSALQLLERFMFPSQMHYQYVSKLSGGERRRLYLLTILMK
                     NPNFLILDEPTNDLDVFTLTALEDYLENFEGCVLVVTHDRFFMDRIVDHIFYLPGAGE
                     VKDISGNYSDFREEMKAYQSEERRQVQADRVDKAEKKAEAARDKEKKKLTFKEKFEFD
                     QLDKEIPLLEKDRKELEARLSNGALSVDELSELSSKLGVLMSTIEEKTLRWMELAELQ
                     E"
     gene            4866..>5972
                     /locus_tag="PK610_06830"
     CDS             4866..>5972
                     /locus_tag="PK610_06830"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRJ35683.1"
                     /translation="MRLFLFFFVLFFFGFTSANAALVKGIVRDEAGEPMPYVNVSVKG
                     KSTGTTTDSKGQYQIQLSAGTHTLIFTFVGFEKQEKTFSLRANETLQLDISMVSGTQY
                     LTDITVASDYKDFAREIVRKAREYRRNQRDRLKNYSVSGYQRVSLEKEYNKKFADSIA
                     GSDSTFEVKDTVKRIKETNYLVESVSTFSFDPPGKTREDFFAFFDHSDVRTGDPLFRG
                     GNVGFTIEYGEGSIAPSLEQEANPYVVLRNEEVYFFDIYDNLINAPGICTKQLLSPLA
                     ASAALNYSIDYAGETEYLPGKKAYRIKVKPVFPAEALFEGTLLIADSSFALVGCDLTI
                     NPRVLTFCRDFRVKIKYEEVSPGFFLPVKREFIYT"
BASE COUNT         1684 a         1189 c         1383 g         1716 t
ORIGIN      
        1 ataaagaatt tgaaatgcag caatcggtaa aagcgctgat tcgaggtggt ttcatcaatg
       61 ctgcacatga tatttccgat ggcgggttat gggttacgct tgcagaaatg ggaatgccac
      121 gtggattagg atttgatatc gaatccgatt ctgaattgcg tctggatgca tttctcttcg
      181 gcgaatctca gggtagagta gtggttactg ttagtgaaga agcgcaggct gaattcatcg
      241 agtttatggc tacacaggaa actcctttta ctttactcgg acatgttacc aaaggtaaag
      301 tggtggtaga cggagaacat tatggttttg ttcatgaagt aaaacctgta tatgataatt
      361 cactcggtga aaagataatg agcaactgat atggccagaa caagacggac tcctgaagag
      421 caagacgcta ttcgtgctat ggtagcggaa aagcaaaccg aaatgattgc tgcaaagggc
      481 gataaggcag ttcgcgaagg aaggatcatt atcatcattt tgtctgtact ggttgctatt
      541 gccgctgttg cagaatattt catgtacgga gaagatccga ttatattagc tgtgtatgct
      601 cctttcattt tggcttttgt agcgtttgcc attttttatt accgcaatcc gtttgcactt
      661 tccattgcag cactttcggt atatggcttt atgcaaattg caggtgccct agcggatccc
      721 acgaatcttg cgaagggaat cattatcaaa gtgattatca ttgcaggatt gatcaaggcc
      781 attaaaagtg ccagggattt tagggttcga aataaaaacg aacaaaaaac gggatcggat
      841 attctggatc aggattttta atccttatac gcgaactccg aaaaagcaga tgtcatccac
      901 ttgttcgtag tcgcctttcc attgcaggtg agcttcgtgc aataattgat gttgcaacgc
      961 aattggattc acagcaaccg atcgcagcaa ttcctttaag cctgaacttt tgaatttctt
     1021 tccgtctttt ccgccaaact gatctgcaaa accatcactg aaaagataaa tcaaatcgcc
     1081 tttttctaat tgaatcgtat gacttggaaa gggagttggg tcgtctgttt ttcctatggc
     1141 ctgtttatgc gctttgatct cctgaattgc agctgtattt gaacgcacaa tccataaggg
     1201 attatttgca ccggcccagg taagaactgc tttcgattta tccaacactc ccagagaaac
     1261 atccatgcca tcgcgaactt ccgattgctt cgattgaaat gtttcagtga caagtgtgcg
     1321 gacatgattt aagatttctc cgggatcggt aatgttcatt tcgcgaacaa cggattgcaa
     1381 ggcgttgtgg caaaccacac tcaccattgc gccgggaacg ccatgaccgg tacaatcgca
     1441 taccgcaaaa aagattttgc ctttatgttc cagcacccaa taaaaatctc ctgctacaat
     1501 atcttttggt agataaagaa taaaatgctc cggaaagatc gtgttcaata catccttgga
     1561 aggtagaatc gcatcctgaa ttctgcgtgc ataggtaatg gaatcggtga tctccttgtt
     1621 tttgtgttcg atgatatggt tctgctcggt gagcagggca ttcgtttgct gcttccgttt
     1681 gttggaacga aacaagtgga tcgacatcac gatgaccagt aaaaaaagta atgcaccgcc
     1741 aatgatgatg atgttttgag tacgtatctg ctcgtgctgc ctatcctgat cgagatcctg
     1801 aatgcgcgat ttctggcgta gtaattcgat ctctttcgat ttccgctcat tctccattcg
     1861 gtaactcata tggttgagtt tttcaagagc agaggcttca tttaattcat ctttcagagc
     1921 cgcataattt ttgtattcgg ctaaactttt atgcagtaat cccatttttt cataaagacc
     1981 cgataaactc aaattgatct tcatgatatg atctttcatt cccaccattt ccgccttttc
     2041 ccgggcactt aaatagtatt tttcggcttg ataataatcc tgatagtcgg taagcatatc
     2101 cgcatagatc tgttcgacct gaaccagatc gtaaggtgat ttacgaatgc tcagtaattc
     2161 aagtgcaaat tcaatttctt cgatggcttg ttttttcttt ccttgtttgg aatgcacccg
     2221 cgcaagtact gcggtgtttg aggataacag atcggtaatt gcatactcct tgcaatagct
     2281 gttcccgatt tcaagaaaat attctgcaga atccaatttg ttctcctcca gataatgggt
     2341 tcccagattg cttgttacca gggctatgct tcgtttgttg ttttcaagct tgaaataacc
     2401 cagtgctgta aaaagtaatt ccttcgcttt tttccgattt cctcttaatc cttcaagatt
     2461 ggaataattg ctcagggtag tagccaatcc ggatttgtca ttgatcgctt cgtgggcttt
     2521 tatggatttt tcatagagtt ccgctgcaat tgataaattc cctttgtcct gatgaatcgt
     2581 tgcaagtgtt gtgtaaacaa ttcccagctg acttctatca ttagtgcttt gcgcaagttc
     2641 caatgccaga ttgccgtaat acaatccact gtcaagatcc gaataactga tgttccagga
     2701 gatcttattc aggaagaaaa tacgagcgga atccgatttg gcttgtttca gatcctcctt
     2761 tgcccggttt acgatttcca ttcggtcctg agcccggagt gtgccgatgc agcagaataa
     2821 agtaataacc agaaggcgaa tcatggatgc taatataatt actttatact ctgtgttttc
     2881 tgctgacatg atcttttgtg ctttctttgc aggatgaatt atttgtctgt cgaggagctt
     2941 agtaaaaatt acggggaaaa gaacctgttt tcgggaatca gttttggtat tgatcagggg
     3001 cagcgtgtag ccctggtggc aaagaacggt gcggggaaat caacactcat gaagattctt
     3061 gccggaaaag aggttgccga ttcgggaaag gctgttttca gaaaagattt gagaattgga
     3121 tttctcgagc aggataaaca gtttactccc ggaaccaccg tgattgaatc ggcgctcgac
     3181 agtgatgatc ctgctgcttt ggcagtgaaa aattatactg cggcaacgca cagtggtaat
     3241 gaggaacagt tgcaacaggc tatggatagt atgaccgcaa ccaatgcctg ggattatgag
     3301 acacgggtag cacagattct cggtaagctc ggactccatg atgccactca gttggtcgac
     3361 tccctgagtg gaggacaaac caaacgattg gccctggcgc aggtgcttat tcgtcagcct
     3421 gaattcatga ttctggacga gcctacgaat caccttgatc tggatatgat cgaatggctg
     3481 gaagtttttc tgaacggatc ggagatcacg cttttcatgg ttacgcacga tcgttatttt
     3541 ctcgagcggg tttgcaatga gattgtggag ttggatggcg gacaattata ccgatacaaa
     3601 ggcaattatt cctacttcct tgagaagaag gccgaacgtg agatgaatga gcagtcggaa
     3661 atctcgaagg caaaaaattt ataccggaag gaactcgatt ggatgcgaag gcaaccaaaa
     3721 gcacgcggaa caaaagcgag ggcgcgtata gacgcatttc acgatacgga atcgatagca
     3781 tcaaagcgaa tcaagaaaga tgagatcgaa tttaatgtaa acatgcaacg catcggtgga
     3841 aagattctgg agttccacaa agtgaaaaag caattcggcg aacgcatcat tttgcaggga
     3901 tttgattatt tgtttaagcg cggtgaaaaa atcggaattg ttggtccgaa tggagtaggg
     3961 aagaccacct ttctcaaaat gatcatgggc caggaagaac ccgacggagg caagataaca
     4021 ttgggtgaaa cggtagcgat tgggtattac tctcaggagg gaatgaagct gaatgaagac
     4081 aaacgcgtaa ttgaagtaat acgggaaatt gcagagttca ttcccctagc aggaggaaaa
     4141 aagatgagtg cattgcaact gcttgagcgc ttcatgttcc cctcacagat gcattaccaa
     4201 tatgtatcga aactgagtgg gggtgagcgc aggcgattgt accttctcac cattctgatg
     4261 aagaatccga attttctgat tctcgatgag ccaaccaacg atcttgatgt gtttacactg
     4321 accgcgcttg aagattacct ggaaaatttt gaaggatgcg ttcttgttgt tacccacgat
     4381 cgatttttta tggatcgtat tgtcgatcat attttctacc tccccggtgc aggtgaggtg
     4441 aaggatattt caggtaatta cagcgatttc agagaagaaa tgaaggctta tcagtcggaa
     4501 gaacgacgac aggttcaggc agatcgtgtg gataaggcag aaaagaaagc agaagcagct
     4561 cgcgataaag agaagaaaaa attaacgttc aaggagaagt ttgaattcga tcagctcgat
     4621 aaagaaattc cattgctcga aaaggaccgc aaggaactcg aagcccgctt atccaacggt
     4681 gccctgagcg tggatgagtt gtcggagctg tcctctaaat tgggggtatt gatgtcgact
     4741 atcgaagaaa aaaccttgag gtggatggaa ttggctgaac tacaggaatg accatttatc
     4801 acatccacgg attttatccg attgattgct gtaacttttc ggcggtttaa tcgtctgaca
     4861 accaaatgcg cttatttcta ttctttttcg tgctattctt tttcggattt acttccgcaa
     4921 atgctgcatt ggtaaaagga attgtacgtg atgaagcagg cgaaccaatg ccctacgtaa
     4981 atgtttcggt aaaaggaaaa agcacaggaa ctacaaccga ctccaaaggg caataccaga
     5041 tccaattatc tgccggaaca cacacactga tttttacgtt tgttggattc gaaaaacagg
     5101 aaaagacatt ttctcttcgt gcaaatgaaa cccttcaact cgatatttca atggtatcgg
     5161 gcacacaata cctcaccgat ataactgttg cgtccgatta caaggatttt gcaagggaaa
     5221 tcgtaagaaa ggcaagagaa taccgacgca atcagcgcga ccgcttaaag aattattctg
     5281 ttagcggata tcaacgtgta tcactggaaa aggaatacaa taaaaagttt gcagacagta
     5341 ttgccggtag tgattctact ttcgaagtga aggataccgt aaaaaggatc aaggaaacca
     5401 attacctggt tgaatcggtt tccacatttt cttttgaccc accgggaaaa acaagggaag
     5461 atttttttgc attcttcgat cattctgatg ttcgaaccgg tgatccgctt tttagaggag
     5521 gaaatgttgg tttcacaatc gaatatggag aaggttccat tgctccctct ctcgagcagg
     5581 aagcaaatcc ttatgtggta ctgcgcaatg aagaggttta tttttttgat atttacgata
     5641 acctgatcaa tgctccgggc atctgtacta aacaattgct atctcctctt gcggcatcag
     5701 cggccttgaa ttacagtatc gactatgccg gagaaacgga gtatttaccc ggtaaaaaag
     5761 cctaccggat caaggtaaaa cctgtctttc ctgctgaggc tttatttgaa ggtactttgt
     5821 tgattgccga tagcagtttt gcgttggtag gatgcgatct caccatcaat cccagagttt
     5881 taaccttttg cagggatttc cgggtaaaaa taaaatacga agaagtgagc ccgggatttt
     5941 tccttccggt aaaacgcgaa ttcatttata cc
//
LOCUS       DAOMYG010000169         5972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4535_length_5972_cov_7.031604, whole genome shotgun sequence.
ACCESSION   DAOMYG010000169 DAOMYG010000000
VERSION     DAOMYG010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4535_length_5972_cov_7.031604"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1040)
                     /locus_tag="PK610_06835"
     CDS             complement(150..1040)
                     /locus_tag="PK610_06835"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRJ35684.1"
                     /translation="MKNLIPFIVIALFASCSGGFSGVEGEEEKADSLGAKHQDTSVNA
                     TLPDIDSMLQKDVMDTTVFDQKSVKELRSPGGVSIEILERTEQGTSIGKGDVVRIKYK
                     GKLPDGTVFDSSDPIGMPLPYFVGVNMSVKGWDDALPLLQTGDKVRMKVPAKLGYGKK
                     GYGKLIPPNTDLVFEMEIVDKMKGDKRPSGLVVYRTVERSGALIKDAQAVSIHFYGWI
                     YSTGKLFDSSHQYGKTHDLVIGLERTLPCWDEALKTMRKGEKAILFSPAALAYAEKGI
                     PEVVPANADLVFSIEVVDVK"
     gene            complement(1037..1570)
                     /locus_tag="PK610_06840"
     CDS             complement(1037..1570)
                     /locus_tag="PK610_06840"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRJ35685.1"
                     /translation="MHKFTILFFVTVLMFSCKEKAPDVIEVKYEDIKENMIEMNRGWT
                     EEEEQIIRNYIRRRNWKMTTSGTGIRYMVYKKGDTLSPLAKEGDIVFVNYEIRLLEGD
                     TLCYSSEGEPESFMVAMDHVENGMHEVVTYLRKGDKAKVIMPYNRAYGLIGDMDKIPP
                     QAPLVYDLEVMDIKKAE"
     gene            complement(1563..2567)
                     /locus_tag="PK610_06845"
     CDS             complement(1563..2567)
                     /locus_tag="PK610_06845"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRJ35686.1"
                     /translation="MIPAGKIAELIAKAEHIVITAHKSPDGDAVGSTLGLFHALTHAG
                     KDCNVILPDGFAQFIAWMPGAGKILLHDKEPDRCAELISQSDLIFCLDYNSLDRTGNL
                     ASLLVEATAPRVLIDHHPFPSDEFAVRFSDTAHSSTCEMVTELLLAMDMKQHITRDAA
                     QCLYSGLVTDTGSFRYSCTSPRTHQIAAELIGTGIENAKIHNQLFDTQSEGRLKLLGY
                     ALSEKMVVDKANQTAWFVLSKAELKRFNHQKGDTEGLVNYGLAIEGIRAAAIFVEHED
                     KVKISLRSKDSVPVNLIAKEYFSGGGHTNAAGGIWESDMDSLIEKWKSIYPAFMATHA
                     "
     gene            complement(2630..3280)
                     /locus_tag="PK610_06850"
     CDS             complement(2630..3280)
                     /locus_tag="PK610_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35687.1"
                     /translation="MRFSAIILLTALLSPVVVQAQKCKGVQFECTNVRKEEGKKKGTK
                     GEPAWTVTGQSRYGNFAYGDTTEVSFTVFKNVNYRISLCSLDPQIQGKIMFQIVERKT
                     QAQTEKYMVTEEVPKTDKDGNILVDQDGNPIMETRTVEKTRRVYGKKPVTRYNNAEDS
                     NKQWVEFTTDQDRTLIIRVVLPSVGDKPKNSLSPQGFACVGLLIEQQMGPQAGGWK"
     gene            complement(3303..4190)
                     /locus_tag="PK610_06855"
     CDS             complement(3303..4190)
                     /locus_tag="PK610_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35688.1"
                     /translation="MNKGSRIVLAAALMSATIAGCGGGEETSSVTTVVDTNQVVEPTE
                     PKVFYQIPTPNEFFSVIKEIGGKSRPELMNPVANTEKYTETRFKALNFGIYSADLAYA
                     SCYEIGQSALEYFKAVEKMGNELDISSAFDESVFKRIEQNLTEGDSLVSISNETYFDA
                     YNYLEENDRGNILALVVAGGWVESIYVVSNLAGPYKDKNPLIERMAQQGHTLTNVIEF
                     MRKYNDDPAVGMTIAQLEEIQMIYETLEVVEKEITTENTGDKLNFSGNAEFKFSKETY
                     EDLTNKVKELRTKYVEAKN"
     gene            4330..4749
                     /locus_tag="PK610_06860"
     CDS             4330..4749
                     /locus_tag="PK610_06860"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRJ35689.1"
                     /translation="MATNRTFTMIKPDGVENKNIGKILDLITNSGFRIIAMRYTRLTT
                     EQASKFYEVHKERPFYGELVEYMTSGPIVAAILEKDNAVAEFRKLIGATDPTQAEPGT
                     IRKLYAESKAKNAVHGSDSDENAEIEGNFFFSGFERF"
     gene            complement(4792..5085)
                     /locus_tag="PK610_06865"
     CDS             complement(4792..5085)
                     /locus_tag="PK610_06865"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HRJ35690.1"
                     /translation="MKRKSNQTTLKAAIERYLEAFKLDSKMDQMDVKSSWESVMGTPI
                     ARRTKDLYFKDKTLFVLLDSSVLREELMMGKTRIIQLLNEHAGKAVVEDIVFK"
     gene            complement(5082..>5972)
                     /gene="recF"
                     /locus_tag="PK610_06870"
     CDS             complement(5082..>5972)
                     /gene="recF"
                     /locus_tag="PK610_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRJ35691.1"
                     /translation="VQGLYEEKGESVEVSCSQKKGQKKSFKRNKKEYDRLSDHIGQIP
                     LVMISPADADLINEGSEVRRKFIDTIISQYDRHYLEQLIDYNKVLAQRNALLKRFGET
                     GSYSQELLEVWDIRLVELARTIHANRHQFLSGFSVLFTRFFRIISGGKEEVTIDYKSD
                     LSEDDFFSVLKRSLPRDRATEYTQVGIHKDDLIFKMGGHPIKRFGSQGQQKSFLIALK
                     IAQYYHLLEVKGKAPILLLDDIFDKLDEHRVSRMMEMVTAPGFGQVFVTDTDREKLPR
                     LLDQLKVEHKEFIIEQGALA"
BASE COUNT         1615 a         1392 c         1179 g         1786 t
ORIGIN      
        1 cgtcggcggc cttgacggcg tcgccgaggc cgatgcccag cgcgagcagc agggtcttaa
       61 aatgtgcaaa ggaatttagc taataaattt ttccttgtaa atgaatcgga taatcttaaa
      121 aaagaaagat tgaaaaaacc ggaattccct tacttcacat ctacaacctc aatgctgaaa
      181 acaagatccg catttgcagg tacaacttca ggaattccct tttccgcata tgcaagggca
      241 gcgggactga ataatatcgc cttttctcct ttacgcattg ttttcagtgc ttcatcccaa
      301 caaggcagcg tgcgctcaag tccgattacc aggtcatgcg ttttgccgta ttggtgagat
      361 gaatcaaaca gcttaccggt agaatagatc catccgtaaa aatgaatcga cacggcctgg
      421 gcatctttta tcaaagcacc ggaccgttca acagtacggt atacaactaa tccggaagga
      481 cgtttgtcac ctttcatttt gtcgacgatt tccatttcga aaacgagatc ggtatttgga
      541 ggaatcagtt ttccatatcc ttttttccca taacccaatt tcgccggaac tttcattctg
      601 actttatctc cagtttgcaa caagggcaga gcatcatccc aacctttaac cgacatattt
      661 acacctacaa aataaggcag aggcattccg ataggatcac ttgaatcgaa caccgttcca
      721 tccggcaatt tccctttgta tttgatgcga actacatcac ctttcccaat ggaagtccct
      781 tgttctgtgc gctcgagaat ttcaatactt actccacccg gcgaacgcaa ttccttaaca
      841 cttttctgat cgaacaccgt agtatccatt acatcctttt gcaacataga atcgatatca
      901 ggcaaagtgg cattgacact ggtatcctga tgttttgctc cgagtgaatc tgctttttct
      961 tcctcacctt ctactccact aaatcctccc gagcaggaag caaataatgc gatgacaata
     1021 aatggaatca gatttttcat tcagcttttt ttatgtccat tacttcaaga tcgtaaacca
     1081 aaggagcttg cggaggaatc ttatccatat caccaataag tccgtatgca cggttgtaag
     1141 gcatgatcac ttttgcttta tcacctttac gcaaataagt caccacttcg tgcattccgt
     1201 tttcaacatg gtccattgcc accataaatg attcgggctc tccttctgat gaatagcaaa
     1261 gcgtatcacc ttccagcaga cggatttcat aattgacaaa tacaatatca ccttctttag
     1321 caagaggaga aagtgtgtcg ccctttttgt aaaccatgta gcgaatccct gttcctgagg
     1381 ttgtcatttt ccaattgcgg cggcgaatat aattccgaat aatctgttct tcctcttctg
     1441 tccagccacg attcatttca atcatatttt ccttgatgtc ttcgtatttc acttcaataa
     1501 catcaggcgc cttctccttg caggaaaaca tcaatacggt aacaaaaaaa aggattgtga
     1561 atttatgcat gtgttgccat gaatgcgggg taaatactct tccatttttc aatcagcgaa
     1621 tccatatccg attcccaaat accaccggca gcattggtat gaccgccacc ggaaaaatat
     1681 tcttttgcaa taaggtttac gggaactgaa tcttttgaac gcaatgaaat tttcacttta
     1741 tcctcatgtt ccacaaaaat cgctgctgca cgtattcctt caatggctaa tccgtaattc
     1801 accaatcctt ctgtatcgcc tttctgatga ttgaatcgtt tcaactctgc tttgcttaat
     1861 acgaaccatg cagtctgatt ggccttatca actaccatct tttcagataa tgcatagcct
     1921 aataatttca atcgtccttc agattgtgta tcgaagagtt gattatgaat ttttgcattt
     1981 tcaattccag ttccaattaa ttctgcagcg atctgatgag ttcgtggcga agtgcaggaa
     2041 tagcggaatg aaccggtatc tgtaaccaaa ccactgtata aacattgtgc tgcatcgcgt
     2101 gtgatgtgct gcttcatgtc cattgccaac agtaattctg ttaccatttc gcaggtagaa
     2161 ctgtgggctg tatctgaaaa ccttacggca aattcatcag atggaaaggg atggtgatcg
     2221 atcaataccc gtggcgctgt tgcttcaact aaaagcgaag ccaggtttcc ggttctgtcg
     2281 agggaattat aatcgaggca aaaaatgagg tcgctttgtg aaatcagttc cgcacaacga
     2341 tccggttcct tatcgtgcaa caaaattttt cctgctcctg gcatccaggc aataaattgt
     2401 gcaaagccat cgggtaagat cacgttacaa tcctttcccg catgagtaag tgcatgaaat
     2461 aatcccaggg tagaccccac tgcatctccg tcaggactct tatgagcagt aataacgatg
     2521 tgttctgctt ttgctatcaa ctctgctatt ttccctgctg gtatcatatt ggactgaata
     2581 aaaaaacccg ttctaataca gaacgggttg aaatgatttt gttacacaat tatttccacc
     2641 caccggcttg tggtcccatt tgttgctcga tgagcagacc aacacaggca aaaccttgtg
     2701 gtgaaagtga attctttggt ttatccccta ctgaaggcaa tacaacacga atgattaagg
     2761 tacgatcctg gtcggttgta aattctaccc attgcttatt ggagtcttct gcgttattgt
     2821 agcgggtcac cggtttttta ccgtaaacac gacgcgtttt ttcaactgta cgagtttcca
     2881 tgatggggtt accgtcctga tcaaccaaaa tatttccgtc tttatcggtc ttgggaactt
     2941 cttcagtaac catgtacttt tcggtttgag cctgtgtttt gcgctcaacg atctggaaca
     3001 tgatctttcc ctggatctga ggatcgagtg aacataaaga aatacgatag ttcacgtttt
     3061 tgaatactgt aaatgaaact tcggtggtat caccgtaagc aaagttaccg tagcgcgact
     3121 gaccggttac tgtccatgca ggttcgcctt tcgtgccttt cttttttcct tcttccttac
     3181 ggacgttggt gcattcgaat tgaacgcctt tgcacttttg tgcctggacc accaccggtg
     3241 aaaggagggc agtaagaaga ataatagctg aaaatctcat atctaactct ttacggtatt
     3301 tattagttct ttgcttctac atattttgta cgcagttcct taaccttgtt ggtaaggtct
     3361 tcgtatgttt ccttgctgaa cttgaattca gcatttcctg agaagttcag tttatcaccg
     3421 gtattctcag tagtaatttc cttttctaca acttcaagcg tttcgtagat catctggatc
     3481 tcttcgagct gagcaattgt catgccaacg gcaggatcat cgttgtactt acgcatgaat
     3541 tcgataacgt tggtaagggt atgtccctgt tgtgccatac gctcgataag cgggtttttg
     3601 tctttataag gacctgcaag gttagatacc acatagatac tttccaccca tccaccggca
     3661 actaccagtg cgagaatgtt accacggtcg ttttcttcca ggtagttata ggcatcaaaa
     3721 taggtctcat tggaaatgct taccagcgaa tcaccttcag ttaggttttg ctcgatgcgc
     3781 ttgaaaaccg attcatcaaa tgcagatgaa atatcaagtt cattccccat tttctcaacg
     3841 gccttgaaat actcaagtgc agactgacca atttcatagc aggatgcgta ggcaaggtcg
     3901 gcagaataaa taccaaaatt gagcgccttg aaacgggttt cggtatactt ttcagtgttc
     3961 gcaacagggt tcatcagttc aggacgactc tttcctccga tttccttaat tacagagaaa
     4021 aattcgttag gtgttggaat ctggtaaaat accttgggtt ctgtaggctc aactacttga
     4081 ttggtatcaa caacagtggt gacagatgaa gtttcttctc cgcctccgca accagcgatt
     4141 gtagccgaca tcagtgcagc tgcaagaacg atccgggatc ctttgttcat gggtttgtct
     4201 gggttaaatg aggtaacaaa aatagtaata tttccattcc tgcaacggtc gggccaaagg
     4261 ttgttgagga ttgtccggag ccggtatttt tatacctttg cgccctgatt tgaaaattta
     4321 agcaaagcta tggctaccaa cagaacattt actatgatca aacccgatgg tgttgagaac
     4381 aaaaacatcg gaaaaatcct tgacctcatt accaattccg gattccgtat tattgcaatg
     4441 cgttataccc gtttaacaac cgaacaagct tcaaaattct acgaagttca caaagaacgc
     4501 cccttttacg gtgaactggt tgaatacatg acctccggtc ctatcgtagc cgcgattctt
     4561 gaaaaagaca acgcagtagc cgaattccgt aagctcatcg gagctaccga tcctactcag
     4621 gctgagcccg gtacaatccg caaactgtat gctgaatcaa aagcaaaaaa tgcagtacac
     4681 ggttccgaca gtgatgagaa tgctgagatt gaagggaact tcttcttcag cggtttcgaa
     4741 agattctgaa tctaatatcc ctgaagcggg tgattgacac tcgcttcggg gctatttaaa
     4801 cacaatatct tcaaccactg cctttcctgc gtgctcgttc agtagttgaa taatacgggt
     4861 ttttcccatc attaattctt cgcgtagcac agaagaatcg aggagcacaa acaatgtttt
     4921 atccttaaaa tacaaatcct ttgtacgacg tgcaatagga gttcccatta cactttccca
     4981 ggatgatttc acgtccatct gatccatctt actatccagc ttaaaggctt cgagataacg
     5041 ttcaatggcg gcttttaagg ttgtttgatt gcttttccgc ttcatgccaa tgccccctgt
     5101 tcgataataa attctttgtg ctcaactttg agctgatcga gtaatcgcgg tagtttttcc
     5161 cggtcggtat ccgttacaaa cacctgacca aatcccgggg ctgtaaccat ttccatcatc
     5221 cgtgatacac ggtgctcatc cagtttgtcg aaaatatcat ccagcaataa aatcggcgct
     5281 ttccctttta cctctaataa gtgataatac tgggcaattt ttagcgcaat caaaaaactc
     5341 ttctgctgac cctgtgaacc aaagcgctta atggggtgac cacccatttt aaaaatcaga
     5401 tcatccttgt gaattcccac ctgtgtatat tccgttgctc tatcacgggg aagtgaacgt
     5461 ttgaggacag aaaaaaaatc atcctcgctg agatcggatt tgtaatcgat ggttacctct
     5521 tccttaccac cggaaatgat tcggaaaaaa cgggtaaaca gaacagaaaa tcctgacaaa
     5581 aactgatgac ggttggcatg tatagttctt gccagctcaa ctaaacgaat atcccaaacc
     5641 tcaagcaatt cctgactgta tgaacctgtt tcgccaaagc gtttcagcaa ggcattgcgc
     5701 tgggcaagta ctttgttgta atcgattaac tgctcgaggt aatgacggtc atactgactg
     5761 atgatggtat caatgaattt tcttcgcact tcgctcccct cgttgatcag gtcggcatca
     5821 gcgggagaaa tcattacaag gggaatttgt ccaatatgat cgctcagccg gtcgtactcc
     5881 tttttatttc gcttgaaact ctttttctga ccctttttct gggaacagga aacctctact
     5941 gactccccct tttcttcgta cagcccctgg ac
//
LOCUS       DAOMYG010000170         5954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4547_length_5954_cov_8.550941, whole genome shotgun sequence.
ACCESSION   DAOMYG010000170 DAOMYG010000000
VERSION     DAOMYG010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4547_length_5954_cov_8.550941"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PK610_06875"
     CDS             complement(<1..406)
                     /locus_tag="PK610_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRJ35692.1"
                     /translation="MKIIVPMAGLGKRMRPHTLTVAKPLIPVAGKPVVQWLVEDIIRV
                     CGQKVDEIAYVVGRFGKENEDRLLKIAADLGAKGSLHYQDEPLGTAHAILCAAEALNG
                     PVVVAFADTLFRADFTLDGSKDGIIWVKKVDDP"
     gene            complement(592..2763)
                     /locus_tag="PK610_06880"
     CDS             complement(592..2763)
                     /locus_tag="PK610_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013750809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRJ35693.1"
                     /translation="MKRVYYLSLFSFTLIACGGEKTEPTAAPSILGDTLYAKPAVSYP
                     LTRTENVTDTYFGTTVEDPYRWLENDTSAETKNWVVEQNKTTYSYLEQIPFRDGFKKR
                     LEEIYNYERSGAPAKVGEYYFYYKNDGLQNQSVIYVRKGLDGKENVFMDPNAMSTNGT
                     VSIGLMGHSKDNKYIAWSRSDAGSDWQEIHVTEIETGKELPDVMKWVKFSDATWYNDG
                     FFYSRYPAPKKGMELSATNAFHSVYYHKLGDPQEKDVLIFEDRANPLRYHYVSLTEDD
                     QYLVLYAASGTNGYECHYKATDLKKGGFTALFTGFENKSSIIDHKDGFFYVTTDIDAP
                     TYRLVKIDPKNPAKENWKDIIPAGKDLLEGVSTGGGKLYAQFLKDVSTHVYQYDYSGK
                     MEKEIEFPAPGTAGGFGCRKEEKEMFYTFTSFTYPTTVYRYDIESGKSEVFFKPSLKF
                     NPDDFESKQVFYTSKDGSKIPMFIVHKKGMKPDGKRPTLLYAYGGFNVSLTPSFNVSL
                     IPILENDGIYALANLRGGGEYGEEWHQAGMLGKKQNVFDDFIAAAEYLIKEGYTNSSK
                     LGISGGSNGGLLVGACMTQRPDLYKVAFPAVGVLDMLRYHKFTVGFGWIPEYGSSEES
                     ESSFKNLFAYSPLHNVKEGTNYPATMVLTGDHDDRVVPAHSFKFAAMLQAKHKGDNPV
                     LIRIETDAGHGAGKPTSKVIEETADKWAFFFFNTNSEVIYK"
     gene            complement(3107..5437)
                     /locus_tag="PK610_06885"
     CDS             complement(3107..5437)
                     /locus_tag="PK610_06885"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRJ35694.1"
                     /translation="MFRSIPISIRVTAAFFIISFTAVSVVGFLSYIRGKESLQEASFS
                     RLTLVREIKADQIEEYFHHIADQALTFAEDHTIIEALKGFTKGFQEIKNEPTPLEQRK
                     AELEKYYREEFIPRLDSNIYSKVNIANYLPQEEAAIRLQDIYISSNPFGVKKKHMLDD
                     ARHESFYSKHHSEYHPYIRKYLKKFGFYDIFLIDSTGNIVYTVFKEVDFGTSLENGPY
                     KKSQLAHVYHKAKGSFEPEHVVLDDYAPYDPSYNAHAAFVAAPIREHGHEIGVLVFQL
                     PIDRINDIMTNRHSWQDVGLGKTGETYIVGEDFTLRNQSRFLIEDRENYLKTIASTGT
                     PDSIVTKIRNLGTSIGLQTCKTIGTENALRGEKGTAVFPDYRGVNVLSAYQPLKIKDL
                     HWVLLSEIDEDEAFNDLYALRKLLIIEFISLLFAIGIISYLISRHITLPLKKLTQSAR
                     QLAKGDMETPIEVTSKDEIGILALSFKKMQLAIHKLFTELKEINAGLEEKVKDRTREI
                     QHQKEMVEEKNKEILDSILYAKRLQNAILPPMEKVKEVLPDSFILFKPKDIVSGDFYW
                     MEALSSNGGGKLDDILIAAVDCTGHGVPGAMVSVVGSNGLNRSVKEFGLRKPSEILDN
                     LRSLVEETFSTSESEVKDGMDIALCRINTDKQTVHYSGAHNPLWILRKDAEQIEEIRG
                     DKQPIGKFDYGKPFSNHEFQLAKGDCIFFFTDGFSDQFGGSRGKKFRAAQLMETLLEL
                     RNLPMEAISTELNNRFETWKGNLEQIDDVCVIGVRF"
     gene            complement(5517..>5954)
                     /locus_tag="PK610_06890"
     CDS             complement(5517..>5954)
                     /locus_tag="PK610_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRJ35695.1"
                     /translation="YKGNTPVGEVLGIELGRQIRDSPHFGRIDAIAPVPLHPKKERVR
                     GYNQSLFIANGIASVLETRVEPQLITRVHNNATQTRRNRFERYRNVETVFQVKEPELY
                     YGKHILLVDDVITTGSTLEACCNSLAQSEKDQARVSIATLAVA"
BASE COUNT         1549 a         1290 c         1296 g         1819 t
ORIGIN      
        1 agggatcatc aactttcttt acccagatta taccatcctt acttccatcg agcgtaaaat
       61 cagccctgaa gagtgtatct gcaaaggcaa ccactacagg tccgttaagc gcttctgcag
      121 cgcacaaaat ggcatgagct gttccaagtg gttcgtcctg ataatgtaaa cttcccttag
      181 cgccgaggtc agctgctatc ttcagcaaac gatcttcatt ttctttaccg aaacgaccta
      241 caacatacgc gatctcatct accttctgac cacaaacccg gatgatatct tccaccagcc
      301 attgaactac aggttttccg gcaaccggaa ttagcggttt ggcaacggta agcgtgtgtg
      361 gacgcattct tttaccgagt ccggccattg gtacaatgat cttcatagca taggtttgat
      421 tggtaaattt ccgattaaaa gcaacaagtg ctaatgatcc tgctcagaat tttgaactac
      481 tgaggtttga aaaactattt cggcaggaat gtaataacaa gacaagcatt gaataggttc
      541 aaaaaaagcg gcgatggttt acatcgccgc ttcgtatttg gttaaccgtt gttatttgta
      601 aatgacttca gaattggtgt tgaagaagaa aaaggcccat ttgtcggcag tttcttcaat
      661 gacttttgag gttggtttgc ctgcgccatg accggcatcg gtttcaatac ggatcagcac
      721 gggattatcg cctttgtgtt ttgcctgaag cattgcagca aacttaaacg agtgtgcagg
      781 aacaaccctg tcgtcgtgat cgccggtaag taccatggta gccggatagt ttgtcccttc
      841 tttaacgttg tgcaagggtg aataggcaaa aagattttta aagctggatt cgctttcttc
      901 acttgaaccg tattcgggaa tccaaccaaa tcctaccgta aatttgtgat aacgcaacat
      961 gtcaagcacg ccaacagcag gaaaggcaac cttataaaga tcgggacgct gagtcataca
     1021 tgcgcctacc agtaatcctc cgttcgatcc accggaaatt ccaagtttcg atgagttggt
     1081 atatccttct ttaatgaggt attctgctgc agcgatgaaa tcgtcgaaca cattctgttt
     1141 tttacccaac ataccggcct ggtgccattc ctcaccgtat tctccaccgc ctcgcagatt
     1201 ggctaatgca taaataccat cgttttcaag aataggaata agcgatacgt tgaatgaagg
     1261 tgtaaggctg acgttgaagc cgccatatgc atacaacaat gttggacgtt taccgtcggg
     1321 cttcattcct tttttgtgca cgatgaacat tggaattttg gacccgtctt tactggtgta
     1381 gaaaacctgt ttcgattcga aatcgtcggg attgaatttg agactgggct taaagaaaac
     1441 ttcagatttt ccactttcaa tatcgtaacg gtaaactgta gtgggatagg taaacgaagt
     1501 aaaagtgtag aacatttctt tttcttcttt tctacatcca aatccacctg ctgtaccggg
     1561 tgcagggaat tcaatttctt tttccatttt accgctgtag tcgtattggt aaacatgggt
     1621 gctgacatcc ttcaggaatt gagcgtacag ttttcctccg ccggtgctta ctccttcaag
     1681 cagatctttt cctgcaggaa ttatatcctt ccagttttcc ttcgcaggat ttttcggatc
     1741 tattttcacc aagcggtatg taggagcatc aatatcggta gtcacataaa agaaaccgtc
     1801 tttatgatca atgatgctgc tcttgttttc gaatccggta aataaggccg tgaatccacc
     1861 ttttttcaga tcagttgctt tgtaatgaca ctcatatccg tttgttcccg atgcggcata
     1921 caatacgagg tattgatcat cttcggtgag tgaaacgtaa tgataacgca agggatttgc
     1981 tcgatcttca aaaatgagaa catctttttc ctgaggatca ccaagtttgt ggtaatacac
     2041 gctgtggaat gcgtttgtag ctgacaattc cattcctttt ttaggagcgg gataacggct
     2101 gtaaaagaat ccgtcgttgt accaggtggc gtcagaaaat tttacccatt tcatcacatc
     2161 gggtaattct tttcccgttt caatttcggt aacgtgaatt tcctgccagt cagatcccgc
     2221 atctgatcgc gaccaggcaa tgtatttgtt gtctttcgaa tgtcccatca gtccgataga
     2281 cactgttccg ttggtcgaca tggcattggg gtccataaac acattctctt taccatcgag
     2341 acctttgcgc acataaataa ccgactgatt ttgcaatccg tcgtttttat aatagaaata
     2401 atattcacca accttggcag gtgcaccgct acgttcgtaa ttgtaaattt cttcaagacg
     2461 ttttttaaac ccgtcgcgaa atggaatttg ttcgagatag ctgtaggttg ttttgttctg
     2521 ttccactacc cagttcttgg tttctgcaga agtgtcattt tccagccagc ggtaaggatc
     2581 ttcaaccgta gtgccgaaat aggtgtcggt aacattttcg gtacgggtta agggataact
     2641 cacggcaggt ttggcataca gggtgtcccc tagaatggaa ggggcagcag taggttctgt
     2701 tttttcgcct ccacaggcaa ttagtgtgaa agagaagagc gataaataat aaacgcgttt
     2761 catagagttg atttagcagt actaaaaaaa gaaattagga ctattgatgc aaaaaattgt
     2821 ggggagaggt tgtgggaaat gctggtcggt tgcagtctcc ggcttcgact ccgcttagct
     2881 atcggttttg ggctatcggt catgacgtct agtgaattgg agtgcgcaaa aatcgcatag
     2941 aaaacaaaaa catcatccac cctgacgctc cttatcagtc ggtcagggtc gtgatgtcta
     3001 gtgtaattgg actgcgctaa aatcgcacag aaagtaaaaa catcatccac cctgacgctt
     3061 cttatcagtc ggtcagggtc gtgatgtcta gtgtaattgg agtgcgctaa aatcgcactc
     3121 caattacaca gacatcatcg atctgctcga ggtttccttt ccaggtttcg aagcgattgt
     3181 tgagttcggt gctgatggct tccattggta ggttgcggag ttccagcagc gtttccatga
     3241 gttgtgctgc ccggaatttt ttaccgcgac ttccgccgaa ttgatcagag aatccgtcgg
     3301 tgaagaaaaa gatgcagtcg ccttttgcaa gctgaaattc gtgattgctg aatggttttc
     3361 cgtaatcgaa tttgccaata ggttgtttgt cgccacgaat ctcttcgatt tgttcagcgt
     3421 cttttcggag aatccaaagt ggattgtgtg caccggagta atgaacggtt tgtttgtctg
     3481 tgtttatcct gcaaagtgca atgtccatgc catctttcac ttcgctttct gaggtagaaa
     3541 acgtttcttc aacaagactt ctcagattat ccagaatttc agatggtttg cgcaatccaa
     3601 actcctttac gcttcggttt aatccgttcg agccaacaac actgaccata gcgccgggta
     3661 cgccatgacc ggtacaatct actgctgcaa ttaaaatatc atcgagtttt ccgccaccat
     3721 tcgaagaaag tgcttccatc caataaaaat cgcctgaaac aatatctttt ggtttgaaga
     3781 gaataaagct gtcgggcaat acctccttca ctttttccat tgggggaaga atggcattct
     3841 gcaatcgttt ggcataaaga atggaatcga gaatttcctt gttcttctct tccaccattt
     3901 ctttctgatg ttgaatttcg cgcgttcgat ctttcacctt ttcttccagt cccgcattaa
     3961 tttctttcag ctcggtaaac aatttgtgga tggccaactg cattttttta aagctcaacg
     4021 ccagaatacc aatttcatct ttactcgtta cttcaattgg agtttccatg tcgcccttgg
     4081 caagttgacg cgcactctgg gtaagttttt tcaagggtaa tgtaatgtga cgggaaatca
     4141 gataactgat tattccaatt gcaaaaagca aactgatgaa ttcgataatc aataatttcc
     4201 ttaacgcata aaggtcgttg aatgcttcat cctcatctat ctcactcagt aatacccaat
     4261 gaagatcttt aatttttaat ggttgatatg cactcagaac atttacgcca cggtaatcag
     4321 gaaaaaccgc ggtgcctttt tctcctctta atgcgttttc agtgccaatt gttttgcagg
     4381 tttgcagtcc gatactagtt ccaaggttac ggatcttggt tacaatggag tccggtgttc
     4441 ccgtactggc aattgtcttt aaatagtttt ccctgtcttc aatcagaaat cgggattgat
     4501 tacgtagggt gaaatcttct cccacgatat aggtttctcc tgtttttccc agaccaacat
     4561 cctgccagga atgacggttg gtcatgatgt cgttaatacg gtcgatcggt aattgaaaaa
     4621 cgagtacccc gatttcatgg ccgtgttccc gaatgggagc tgcaacaaat gcagcatgtg
     4681 cattgtaaga tggatcatat ggtgcatagt cgtcgagtac cacgtgttcc ggctcgaatg
     4741 aacctttggc tttgtggtag acatgcgcaa gctgactttt tttgtaagga ccattttcca
     4801 gtgaggtgcc aaaatcaact tctttaaata cggtatacac gatattaccg gtggagtcga
     4861 tcagaaaaat atcgtagaaa ccgaatttct tcaggtattt tctgatgtag gggtgatact
     4921 ctgagtgatg tttggaataa aaactttcat ggcgcgcatc gtcgagcatg tgttttttct
     4981 ttaccccaaa tggattggaa gaaatatata tatcctgtaa tcggatggct gcttcttcct
     5041 gcggtaggta gttcgcaata tttactttcg aataaatatt gctgtcgagt cgcggaatga
     5101 attcttcccg gtaatatttt tcaagttctg cctttcgctg ctcaagagga gtaggttcat
     5161 ttttgatttc ctgaaaaccc tttgtaaatc cttttaaggc ttcaatgatg gtatggtcct
     5221 cagcaaatgt gagcgcctga tcagcgatat gatggaaata ctcttcaatc tgatcggcct
     5281 tgatttctct aaccagtgtg agtcgactaa aactggcttc ctgcagcgat tccttacctc
     5341 tgatgtagga taaaaagcca actaccgaaa ctgccgtaaa actgataatg aaaaaggcag
     5401 cagttaccct gattgatatg gggattgatc gaaacacggt gcaatatagg aaatccccat
     5461 cagccaacag gagaaacccg gactaagcga ttaatagtca ggttagaagg ggtttttcag
     5521 gctacagcca gcgtggcaat acttactctg gcctgatctt tttcggattg ggcaagggaa
     5581 ttacaacagg cttcaagggt tgagcctgtt gtaatcacat catccaccag caggatatgt
     5641 ttaccgtagt aaagttcagg ttctttcacc tggaatacgg tttccacatt tcggtaccgt
     5701 tcaaagcgat ttctgcgtgt ttgagtggca ttgttatgga ctcttgtaat aagctgtggc
     5761 tccactcttg tttccaatac cgatgcaata ccgttagcga tgaacaaact ttgattgtat
     5821 cctcttactc tctctttttt gggatgaagg ggcaccggtg caattgcatc aattctccca
     5881 aaatggggcg aatcgcgtat ctgccttcct agttctatcc ccaacacttc ccctaccgga
     5941 gtattgcctt tgta
//
LOCUS       DAOMYG010000171         5953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4549_length_5953_cov_7.962191, whole genome shotgun sequence.
ACCESSION   DAOMYG010000171 DAOMYG010000000
VERSION     DAOMYG010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4549_length_5953_cov_7.962191"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..669
                     /locus_tag="PK610_06895"
     CDS             217..669
                     /locus_tag="PK610_06895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRJ35696.1"
                     /translation="MARSVNKVILIGNLGKDPEVRRMENNIAKATFALATGETYRDKN
                     TNELKTITDWHNIVVWRGLADVAEKYLKKGMQVYIEGRLKTRSYQDQSGVTKYITEVV
                     ADNLVMLTRGNAESGSTSAATAQGHGNEDMSGGGYEISGPDGGDDLPF"
     gene            690..2021
                     /gene="gldE"
                     /locus_tag="PK610_06900"
     CDS             690..2021
                     /gene="gldE"
                     /locus_tag="PK610_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HRJ35697.1"
                     /translation="MEPPSVHALSDLFLIILHAPSAGTVISMVIIGILLICSAVISAS
                     EVAYFSLTAKDLQTIRESEDPVHRRIAGILEKPQQLLATILVANNMVNVAIVILSTTA
                     LSDMVDFEHSPAWFEFIVEVIGVTAMILIIGEVVPKIYANKFSLRVAGVMSKPLLVLK
                     QLLWPISQMLITSSNFINKRVKKKSSTMTVDELGHALEITLGEGEATQGEKKILEGIV
                     RFGNTDARQIMKPRMDVVAVSIDLNFYGLIALIIDSGFSRIPVYEGTLDTIKGVLYIK
                     DLIPHLNASDDFKWQSLIRDAFFVPENKKIDDLLKEFKDAKIHLAIVVDEYGGTQGIV
                     TLEDVIEEIVGEITDEFDDEKISYSRIDERNLVFEGKTSLIDIYKVIGIEGEEFEKAK
                     GESDTLAGFLLEQSGRMLQAGEKVQFGEYTFTIEAADKRRIKQIKMTLPAE"
     gene            2018..2599
                     /gene="gldD"
                     /locus_tag="PK610_06905"
     CDS             2018..2599
                     /gene="gldD"
                     /locus_tag="PK610_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HRJ35698.1"
                     /translation="MKYSFLKFLVVIGIAFFSACSEEEPGVPKPRGYMRIDFPEKEYI
                     EFRGDCPYTFDFPSYARIDHIGRDTVTWCNKNIEFVPFRGTMHLTYKRLQGNVARFIE
                     ECHNMAYDHSIKATAIERKQFVNDTAQVFGLLYDIQGNAASPLQFYITDSTTHFIRGA
                     LYFQAKPNYDSILPVLTYVREDVERFIESLRWK"
     gene            2729..3082
                     /locus_tag="PK610_06910"
     CDS             2729..3082
                     /locus_tag="PK610_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35699.1"
                     /translation="MSYKEKVTTMYNMVNSGQILDAFEKYYHNDVVMQEMGEAERVGK
                     DANREYEKNFVGSVKAINGGGVDAITSDESNGVTMVESWMDITFQNDARVKLEQVAVQ
                     YWKGDQIVKERFYHK"
     gene            complement(3134..3697)
                     /locus_tag="PK610_06915"
     CDS             complement(3134..3697)
                     /locus_tag="PK610_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35700.1"
                     /translation="MRIREVWFVVVFLHAMLLLLSCNKGKNDPDSCNGKTRRPVKVVI
                     DDRVNQIDTIPIPARLDSLGKIVVPEVKWGTGRQDLELRTYTVRAKVHKLSKERDGDY
                     HIRLIDDNENYLICEAPNPGCSYARQSRYLQNYIRVREFLDQNKKTLEGKTVTLTGVA
                     FIDIDHYYKRKQAENNLELHPVLDIHF"
     gene            complement(3717..5837)
                     /locus_tag="PK610_06920"
     CDS             complement(3717..5837)
                     /locus_tag="PK610_06920"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HRJ35701.1"
                     /translation="MKRLHVIILAAIVSAAACKSGGKSSKVEVSGSDKNTAVAVEAVK
                     EEMPSFTFETVAGTIDFQDMDPKIDPRKDFYSFANGNWVRNNPVPATENRWSSFNILS
                     DDNNRKLRAILEEAAAGKYPKGDHRQLIGDYYASFMDSAKRDKEGIAPIQTDLKKIDA
                     IKSKKELAAVIAHHHNYGIHPLFDTGIDQDLKDINRHMAYISQSGLLLPNKDYYFKSD
                     SSSVKLRNNYIAHLSRMFVFLGYDKTKADKAAASVLDLETQLAEASMGPVEMRNIEAQ
                     YNKMSIADFKKSVPSFDWNKYFNDRGLAKADTIIVGQPAFFKRVEAMITSVSLDVWKD
                     YLKIHFFEAASGKLSSEIEQEVFSFFSTQLRGTKKMKPRWERAINSIGWTAIGEALGH
                     AFVDKHFKPESKLKVNEMVDNIMFVFRGRLDKLEWMSPVTKTKALEKLASFTRKLGYP
                     DKWTDYSTLSITRESYFKNWMEQSKFKVTENTQKLGKPIDRSEWQMAPHIVNAYYNPT
                     WNEIVFPAGIMQPPFFDPNAEDAVNYARMGAVIGHEFLHGFDDQGAQFDATGQFVNWW
                     TSDDSLKFAARTNKLVEHFNSFEALPGIFVNGELTLGENIADLGGLTMSYYAYQRSLE
                     GKERKKINGFTAEQRFFVAFAQVWKENHTESSMKLQVFTNPHSPGKYRVLGPLANMPE
                     FFQAFNVKEGDPMRRAADKISLIW"
BASE COUNT         1759 a         1248 c         1228 g         1718 t
ORIGIN      
        1 acaggcatca gttaagccat cgcactattt atgctcaatt ctcggagatc cgtttaagca
       61 gaaaatttaa gccccctcaa ggctggatcg gtgtagcact gaatgagatg cctgacgttg
      121 ccattccccg tttgatcgag cgctttctgg aagagcattt ctcttcccat ggtgtttagc
      181 tttttgtata tttgttcggc atcattgtag tcagctatgg caagaagcgt taataaagtc
      241 attttaatcg gcaaccttgg gaaggatccc gaagtgcgtc gtatggaaaa caacattgcg
      301 aaagcaacat ttgcattggc aaccggtgaa acctaccggg ataaaaacac caatgaatta
      361 aaaaccataa ccgattggca caatattgtt gtgtggagag gtttggcaga tgttgcggaa
      421 aagtacctta agaaaggcat gcaagtctat attgaagggc gattaaaaac ccgctcttat
      481 caggatcaga gtggtgttac caaatacatt accgaagtag ttgcagacaa tcttgtaatg
      541 cttacccggg gtaatgcaga aagtggatca acatctgcag caactgctca gggtcatggc
      601 aacgaagaca tgagtggcgg cggttatgaa atttccggac ctgatggcgg agatgatctg
      661 ccattttgag ttgaaccaaa gcaaaagcat tggaacctcc ctcggtacac gctctatctg
      721 atctttttct gatcattctt cacgcaccct cagcgggcac tgttatctcc atggtaatta
      781 ttgggatact tttgatctgc tctgccgtaa tttctgcttc tgaagtcgcc tatttttcat
      841 tgacagcaaa agatctccaa acaattcgcg aaagtgaaga ccctgttcat cgccgcattg
      901 ccggaattct ggaaaaaccg caacagttac ttgctacaat tctagtagca aacaatatgg
      961 ttaacgttgc cattgtcatt ctgagcacaa cggccttaag cgacatggtt gattttgaac
     1021 attcaccggc atggtttgaa ttcattgtag aagtgattgg tgtaacagca atgatcctca
     1081 tcattggtga agttgttcca aaaatttatg ccaacaaatt ttcacttcgg gttgcaggag
     1141 tgatgtcgaa accattactg gttttgaaac aactgttatg gcctatttca caaatgctca
     1201 tcaccagctc caatttcatc aataaacgtg tcaagaagaa aagctcaaca atgacggtag
     1261 atgaacttgg tcacgccctt gaaataacac ttggagaagg agaagccacg cagggagaga
     1321 aaaaaattct ggaaggaata gtacggttcg gtaacaccga tgcgcgtcag attatgaaac
     1381 cccgcatgga tgttgttgct gtttccattg atttgaattt ttacggttta attgcactca
     1441 ttattgattc agggttttca cgaattcctg tttatgaagg aacactggat accattaaag
     1501 gagtgctgta catcaaagac ctcattccac atttaaatgc cagtgatgat tttaaatggc
     1561 agagcctgat acgagatgca tttttcgttc ctgaaaataa aaaaattgat gatctgctga
     1621 aggaattcaa agacgcaaaa attcaccttg ccattgtggt tgatgaatat ggaggcacgc
     1681 agggaattgt tacactggag gatgtcattg aagaaattgt tggcgagatc actgatgaat
     1741 ttgatgatga aaaaatttcc tattcaagaa ttgatgaacg caatcttgtt tttgaaggaa
     1801 agacctcact tatcgatatc tataaagtga tagggatcga aggtgaagaa tttgaaaagg
     1861 ccaaaggaga aagtgatacg cttgcaggat ttcttcttga acaaagcgga aggatgttgc
     1921 aggccggtga gaaagtgcag tttggagaat acacctttac cattgaagct gccgacaagc
     1981 gcagaatcaa acaaataaaa atgacacttc ctgcagaatg aaatacagtt ttctaaaatt
     2041 cctggttgta attggcatcg catttttttc cgcatgcagc gaagaagaac ccggcgtacc
     2101 caagccaaga ggatacatgc gtattgattt ccctgaaaaa gaatacatcg aattccgtgg
     2161 agattgtcct tacactttcg attttccttc ctatgcgcga attgatcata taggtcgcga
     2221 tactgttacc tggtgtaata aaaacattga gtttgttccg ttcagaggaa ccatgcattt
     2281 aacgtataaa cgtctgcaag gaaatgttgc ccgttttatt gaagagtgtc ataatatggc
     2341 ctacgatcat tccattaaag caacggccat agaacgcaaa caatttgtga acgataccgc
     2401 gcaggtattc ggattgcttt acgacattca gggaaatgca gcttctccgc ttcaatttta
     2461 tattactgac tccactactc attttattcg tggcgcgctt tatttccagg caaaacccaa
     2521 ttacgattcc atactcccgg tactaacgta tgttagagaa gatgtagaac gctttatcga
     2581 aagcctacgc tggaagtaat tttaaggaaa atttatcatc tcaattgaaa tttaaagcga
     2641 tgataatcaa taatataata aaatattaat tgttgtataa acaactattg tagcaatgtg
     2701 ttatatttgc tgaactaaat tcaaaacaat gagctacaaa gaaaaagtta cgaccatgta
     2761 taatatggtc aattcaggtc agatccttga tgcatttgaa aagtactacc acaacgatgt
     2821 tgtgatgcag gaaatgggag aagctgaacg cgttggaaaa gatgcaaacc gcgaatacga
     2881 aaagaatttt gtcggttctg ttaaagccat taatggtggc ggcgtggatg cgattacctc
     2941 agatgaaagc aatggtgtta caatggtaga gagctggatg gacatcacct ttcagaatga
     3001 tgcacgtgta aaactggaac aggttgcagt tcaatactgg aaaggcgatc aaattgtaaa
     3061 agaacgtttc tatcacaaat aatccctaaa ccctgaaaaa aagaaagcga tgttctcaca
     3121 tcgctttttt tatttagaaa tgaatatcta gcacagggtg tagctcaaga ttattctccg
     3181 cctgtttgcg tttgtaatag tgatcaatat cgataaacgc cacgccggta agtgttactg
     3241 ttttaccctc cagtgttttt ttattctgat ccaaaaactc tctcacacga atataatttt
     3301 gcaaatagcg cgattgtcgt gcgtatgaac aaccgggatt tggtgcttcg catatcaggt
     3361 aattctcgtt atcgtcaatt aaacgaatgt gataatctcc atcgcgttcc tttgataact
     3421 tgtgcacttt agcacgaact gtgtaggtcc gtaattccag atcctgccga ccggttcccc
     3481 atttcacttc tggtacgact atcttcccaa gagaatccaa tcgtgcagga atggggatcg
     3541 tatcgatctg gtttacgcga tcgtcaatta caacttttac cggacgacgt gttttcccat
     3601 tgcaggaatc gggatcattc ttgcccttat tacaggaaag cagtaacaac atcgcatgca
     3661 aaaacacaac tacaaaccaa acctcacgaa ttctcatccc ttaaaagtgc ttacaattac
     3721 cagatcaaag aaattttatc cgctgctctt cgcatgggat caccttcttt tacgttgaat
     3781 gcctgaaaaa actcaggcat gtttgccaat ggtccaagca cacggtattt tccgggtgaa
     3841 tgtggattgg taaagacctg caatttcata ctgctctcag tgtggttttc cttccatacc
     3901 tgagcaaatg caacgaagaa acgctgctct gcagtaaatc cgtttatttt cttacgctcc
     3961 ttaccttcaa gtgaacgttg ataagcataa tacgacatgg ttaatccacc taaatcggca
     4021 atgttttctc ccaaagtcaa ttcaccgttg acgaaaattc cgggtagcgc ttcgaaggaa
     4081 ttaaaatgtt caacaagttt gttggtacgg gcagcaaact tcaatgaatc gtcggatgtc
     4141 caccagttca caaactgacc ggttgcatca aattgcgcac cctgatcatc aaatccgtgc
     4201 agaaattcat gtccgatcac tgctcccata cgggcgtagt taacagcatc ctcagcattc
     4261 ggatcgaaaa acggaggttg cataattcct gcaggaaata caatttcatt ccatgtagga
     4321 ttgtaataag cgttaacgat atgaggagcc atttgccatt cgctgcggtc gatcggctta
     4381 cctaactttt gtgtgttctc cgttacctta aacttcgatt gctccatcca gttcttgaaa
     4441 taagactcac gtgtgatact gagcgtagaa tagtcggtcc acttatcagg atagccgagt
     4501 ttacgtgtaa aagaagccaa tttttcgagt gcttttgttt ttgtaaccgg actcatccac
     4561 tccagcttat ccaaacgtcc gcggaaaaca aacatgatat tgtcgaccat ttcattcacc
     4621 ttcagtttgc tttccggctt gaagtgctta tctacaaatg catgtccgag cgcttcgcca
     4681 atggcagtcc agccgatgga attgattgcc cgttcccagc gtggtttcat tttttttgtt
     4741 ccgcgaagtt gcgtactgaa aaagctgaac acttcctgtt caatttcgct gcttagcttt
     4801 ccggatgcag cttcaaagaa atgaatctta agatagtctt tccaaacatc gagagatact
     4861 gaagtaatca ttgcctctac ccgcttgaaa aatgccggct gaccaacaat gatagtatcc
     4921 gctttcgcca aaccgcggtc gttaaaatat ttattccaat cgaacgaagg aactgatttt
     4981 ttgaaatcgg caatactcat tttattgtac tgcgcttcga tgttgcgcat ttcaacagga
     5041 cccatcgatg cttctgccaa ttgggtttcg aggtcgagaa ctgaagctgc tgctttgtcg
     5101 gctttggttt tatcgtaacc taaaaacacg aacatgcgag aaagatgcgc gatgtaattg
     5161 ttgcgaagtt taactgatga agaatcgctc ttgaaataat aatccttgtt gggcaacaac
     5221 aatccgcttt gcgaaatata tgccatgtgg cgattgatgt ccttcagatc ctgatcaatt
     5281 ccggtatcga acaaaggatg gatcccgtaa ttgtgatgat gagcaataac agcagcaagt
     5341 tctttcttcg atttaatggc atcaattttt ttgagatcag tctgaatggg tgcaatcccc
     5401 tctttatcgc gttttgcact atccatgaaa cttgcataat aatcaccgat cagctgacga
     5461 tgatcgccct ttggatattt tccggctgcc gcttcttcga gaattgcacg aagcttacgg
     5521 ttgttatcgt cggaaagaat attaaaacta ctccagcggt tttctgttgc aggaaccgga
     5581 ttgttacgaa cccagttacc atttgcaaac gaataaaaat ctttacgagg atcgatttta
     5641 ggatccatat cctgaaaatc gattgtacct gcaacggttt caaatgtaaa cgaaggcatt
     5701 tcttccttca ctgcctcaac ggcaacagct gtgttcttat ccgaaccact tacctcaacc
     5761 ttagaacttt ttccacccga tttacaggca gctgcactta caatagcagc aagaataatt
     5821 acatgtagtc ttttcataat tgtagaatga agtccgaaat taatgaattt gaagcaatcc
     5881 cccacaaaaa tgcattctgc taattatccc gctgataccc agcagcactt attctgccct
     5941 aagcattgcg ata
//
LOCUS       DAOMYG010000172         5927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4577_length_5927_cov_12.192439, whole genome shotgun sequence.
ACCESSION   DAOMYG010000172 DAOMYG010000000
VERSION     DAOMYG010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4577_length_5927_cov_12.192439"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..2598)
                     /locus_tag="PK610_06925"
     CDS             complement(121..2598)
                     /locus_tag="PK610_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ35702.1"
                     /translation="MRRFFALFSFVIAASPIVAQINVQWESRFNDIATNTPQIVADMF
                     VDPAGNVYVTGSSYSVANGYNIVTIKYNNSGAQLWINNFNGSGNGLDEARALFVDASG
                     NVYVTGYSFRGGSNYDYTTIKYNSAGAQQWQQYYDAGASLFDDASDIVVDATGNVYVT
                     GSGQIASNNTNIRTVKYNSAGTQQWAANFSSSGSNLDQGKIIALDAAGDVYVAGNAFN
                     TGQDLNYRVLKYNAVTGAQVWTVQYNHTLNSYDYPTDMKLDASGNVYVTGYSYNGAVS
                     DDDIHTIRINSAGTITHTVIFNGTANGADISNAMQIDASGNVYVVGRAKNSGTAEDFL
                     VIKYDANLNQIWSDSYNGLGGNYDQASDIAIDNSGNVYATGYSYLPSSNNDYFTIKYN
                     PTTGARIWTTRFNGTANNSDQAKVINVDASGNVYVSGDSRGSGTGSDYSTIKYCQLFT
                     NAGSDQQICIGGNVQLNATGGSTWNWTPSTGLSSTTIPNPVANPSSTTTYIVSSTDGN
                     GCTDLDTIQVVVNPLPGPVITPDGPTSFCVGDSVKLSASGFTSYSWNTGSMNPEITVM
                     TAGTYTVTVTDAMMCNNSTSVTVTVNSLPTVDAGTVPPVCAGSSVQLQATGAQTYLWN
                     ANPTLSNITIANPIATPNTSQWYYVTGTDANGCSNYDSVQVSLNPLPATPTITFVSAN
                     YTLVTNQTSGVQWYLNGVPVAGGTTQTINVLNNGQYWVTYTNANGCTSINSDTITILD
                     VSVDELEAIGLVVFPNPVSDNLTIKANDVISAAIRVCDLAGRVMIQLPFSTGNRFQVD
                     VSGLVAGTYLILFETESGISVQRIIKQ"
     gene            2750..>5927
                     /locus_tag="PK610_06930"
     CDS             2750..>5927
                     /locus_tag="PK610_06930"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ35703.1"
                     /translation="MNGTLRRYFFAIVFSFAAIIPAGATHIVGGSLTYEHLGGSTYRI
                     LLKLYRDCSPASAPQIALQNSQVIEIYNANGTFNSSVTLPRISLSFVPLNLDTCVADP
                     GICVQEGLYAGIVNNLPPVTGGYHMYFETCCRNSSIVNLVNPLNAGEGFYCRISDNTL
                     LLTNSSPQWVNFPPVFVCQGQPINFNHGATDPDGDSLVYSFYRPLSDIDYLVANDLTF
                     TAGVPNFAFVNYQAGFGFNNPLGGANLTISNGGLVDGIPANIGQYVVGVRCDEYRDGI
                     LIGSIYRDFQFNVVVCPPPALAGIGPVDGCAGSAIQFSNTSSVTANGFQWNFGDGSPV
                     STATNPSHTYPGIGTFTVQLIAQVGTPCADTAYRTFPISFSDASFNRPDSVCINTPVN
                     FINTSTAAPNNTVNSWLWNFGDGSPTSSLPNPNHTFPGGGTYNVQLIVNSTQGCRDTI
                     VRPIFVQGLPLAIAGPDTSACNNNPLVGLSGSIANAAGGIWLNGSGTFIPNNSTLVTD
                     YIPSAAEVAAGQLSLVLATVGNGLCPQDVDSLHIVFIDGPLVNAGPDIQACKDTAYVP
                     LNGSVQFAGGGIWSSSGTGTFSPANDQLNATYIPSTADTLAGSVWLYLTATNVGNCIT
                     TTDSLNLSFYNPPVATILNNNVACSGDPIPLQVNSTTGQGVWTTLGSGLFQPDSSIIS
                     WYQPGANDIATGTVTLIFTSTNNGGCKPARDTINVSIIPSPVPVFTFTEPCFGIASTF
                     DDASTAVGGVTAWSWNFGDGSPLSTTQDPTHLFNTSGTFNVTLIVTSGNGCRDTLTQT
                     VNVHHLPQPAFLTSYPCLNGGTSFQDLSSVMNSTLTTWSWTFGDGGVSANQNPTHQYP
                     ASGSYSVTLTVTSALGCSSSVTQNTNVLPGPNAAFSVSDNSVNQFETVVFTDQSTPAG
                     QITGWLWDFGDNSPQVNTQNSSYAYNGSGTYIVTLVVTDNNGCIDTARNTVIVFLPPD
                     IPNAFTPNGDGSNDFLQIFGGPFRELELNIYNNWGQVIYTTTDATDCGGYVCVGWDGK
                     FNGIDQPMSVYVYQLRAVTEDGVEHILTG"
BASE COUNT         1604 a         1371 c         1191 g         1761 t
ORIGIN      
        1 acccggctta tagataagtc aggatcgatt tatgtcggtg atttccctgc aggattttat
       61 tttttacagc tcaggaataa aggcacctct cagtttgccc gattgatgat tgttcgttga
      121 ttattgtttg ataatccgct gaacacttat tcccgattct gtttcaaaaa gaatcagata
      181 agtacctgca accagacccg atacatccac ctgaaaacga ttaccggtac tgaacggtaa
      241 ttggatcatc acccttccgg caaggtcaca aacacgaatt gcagcagaaa taacatcgtt
      301 tgctttaatg gtaagattat cgcttacagg attggggaaa acgactaatc ctattgcttc
      361 gagctcgtca acactaacat ccagaattgt aattgtatct gaatttatac ttgtacatcc
      421 gtttgcgttg gtatacgtta cccaatattg tccgttgttg agcacgttaa tggtttgagt
      481 ggtacctcct gcaacaggaa caccattcag ataccattgc actccggaag tctggttggt
      541 taccagcgta taatttgcac tcacaaaagt aattgtagga gtagcaggaa gtgggtttaa
      601 gctaacctga acagaatcat aattgctgca tccgtttgca tctgtaccgg taacataata
      661 ccattgactt gtattaggtg ttgcaatggg gttggctatc gtgatattgc taagggtggg
      721 gttagcgttc cacaaatagg tttgcgcccc ggtagcctgc agctgtacag aagatcctgc
      781 acaaacagga ggaacagttc cggcatcaac agtgggcaaa ctatttactg taaccgttac
      841 agaggtcgag ttattacaca tcattgcatc tgttactgtt actgtataag tacctgcagt
      901 cattacagta atttcaggat tcattgatcc ggtattccat gaataggaag taaaacctga
      961 agcagagagt ttaaccgaat ctcccacaca aaaagatgtt ggaccatcag gagtaattac
     1021 cggcccggga aggggattta ctacaacctg aatcgtatcc agatctgtac atccgtttcc
     1081 gtctgttgaa cttacgatat acgttgtggt agatgatgga tttgctaccg gattaggaat
     1141 agttgttgaa ctcaatcctg ttgatggggt ccagttccag gtagaacctc ctgttgcatt
     1201 aagctgaaca tttcctccga tacaaatttg ttggtctgat ccggcattgg taaataactg
     1261 gcaatacttg attgttgaat aatcactacc ggttcccgat ccgcgggaat ctccacttac
     1321 atatacattt cctgatgcat ccacattgat cacctttgcc tgatcgctgt tattggctgt
     1381 tccgttaaat cgcgttgtcc agatgcgcgc tcctgttgtt ggattatact ttattgtaaa
     1441 ataatcgttg ttagaagaag gtaaatagct gtaacctgtt gcatatacat ttcctgaatt
     1501 atcaatggca atatctgagg cctgatcata attcccgcct aatccgttat aagaatcact
     1561 ccagatttgg ttgagatttg catcatattt gattacaaga aaatcttcag cggttcctga
     1621 attttttgcg cgaccaacaa catatacatt tccgcttgca tcaatttgca ttgcgttgga
     1681 aatatcagca ccattggctg ttccgttaaa aattactgta tgtgttatcg ttcctgcaga
     1741 attaattcga atggtatgaa tatcatcatc actcaccgct ccattgtagg aatatccggt
     1801 tacatacaca tttccggaag catccaattt catatcggta ggataatcgt acgaattaag
     1861 cgtatggttg tactgtactg tccagacctg cgcacctgtt acagcgttgt attttaaaac
     1921 gcggtaattc agatcttgac cggtattaaa tgcatttcct gccacataaa catcacctgc
     1981 agcatctagt gcaattattt ttccctgatc aagattgctt ccggatgaag aaaaatttgc
     2041 cgcccactgc tgggttcctg ctgaattgta cttaacagtt ctgatgttgg tattattcga
     2101 tgctatttgt ccagatccgg ttacatacac gtttccggtt gcatctacaa caatatccga
     2161 agcatcatca aacaatgagg caccagcatc gtaatactgc tgccattgct gagctcctgc
     2221 tgaattgtac ttgatggtgg tgtaatcgta attcgaacct cctctgaaag aatatcctgt
     2281 tacataaaca ttgcccgaag catccacgaa aagtgcgcgg gcttcatcta aaccgtttcc
     2341 ggagccatta aaattattaa tccacaattg tgctccgcta ttattgtatt tgatcgtcac
     2401 gatattatat ccgttcgcaa cggaataaga ggatcctgtc acataaacgt ttcctgcagg
     2461 atcaacaaac atatctgcaa cgatctgagg tgtatttgta gcaatatcat taaatcggga
     2521 ttcccactgg acattgatct gcgcaacaat tggagaggca gcaattacaa atgaaaataa
     2581 agcgaaaaaa cgtctcatca gcgaaatggc attagtgtat aacgagctaa tttaaatcaa
     2641 aagttccata acccgaaatc agaacgcttt ttcacgttta gctatccaat ttcttgtctc
     2701 taatggtttt agttttctat atttactcct taaacaaatt cccttatcca tgaacggaac
     2761 tttacgccgt tattttttcg ctatagtttt ttcatttgct gcaattattc cagccggtgc
     2821 aacgcatatt gttggtggtt ctcttaccta tgagcatctc ggtggtagta cttaccgcat
     2881 tttgctcaag ctatatcgcg attgttctcc tgcatctgca ccgcaaatcg cattgcaaaa
     2941 ctcgcaggtc attgagatct acaacgcaaa tggaacattt aattcttctg ttactttacc
     3001 aagaatctca ttgtcatttg tcccacttaa cctcgacacc tgcgtggccg atccgggtat
     3061 ttgtgttcag gaaggacttt atgcaggtat tgtaaacaac cttccgccag ttaccggagg
     3121 atatcacatg tattttgaaa cttgttgccg aaattcttcc attgtaaacc ttgtaaatcc
     3181 tctcaatgca ggagaaggtt tttattgcag aatttctgac aatactcttc tacttaccaa
     3241 cagctctccg caatgggtca acttccctcc cgttttcgtt tgtcagggac aacccatcaa
     3301 tttcaaccac ggagctaccg atcctgatgg tgattcattg gtctattcat tctaccgccc
     3361 actttccgat atcgattacc ttgttgccaa tgatctcacc tttactgctg gtgttcccaa
     3421 cttcgctttt gtgaactatc aggccggatt cggtttcaat aatcctctcg gcggagcaaa
     3481 tttaaccatc agtaatggcg gactggtcga cgggattcct gcgaatattg gtcagtatgt
     3541 tgttggtgta cgttgcgatg aataccgtga tggaattttg atcggaagta tataccgcga
     3601 tttccagttc aacgtagtag tttgccctcc gcctgcactt gcgggaattg gtccggtcga
     3661 cggctgtgcg ggctctgcca ttcagtttag caatacatca tcagtaactg ctaacggatt
     3721 tcaatggaat tttggagatg gctcaccggt ttcaacggca acaaatcctt cgcataccta
     3781 tcccggtatc ggaaccttta ctgttcaatt gatcgcacag gtgggtacgc cttgtgctga
     3841 tactgcctac agaacttttc cgatttcttt ttcagacgca tccttcaacc gaccggattc
     3901 cgtttgtatc aacacccccg tcaacttcat caatacttcc actgctgccc caaataacac
     3961 tgtcaattcg tggctgtgga attttggaga tggctctcct acttcatcat tacccaatcc
     4021 caaccatacc ttccctggtg gaggaacata caacgttcaa ttgatcgtga attctacaca
     4081 aggatgtcgc gataccattg ttcgtccgat ttttgttcag ggattacccc ttgccattgc
     4141 tggtcctgat acttccgctt gcaataacaa ccctcttgtt ggattaagcg gatcgattgc
     4201 aaatgcagct ggcggaatct ggctcaatgg ctcaggtact ttcattccga acaacagcac
     4261 attggttacc gattatatcc ccagtgcagc agaagttgcg gcaggacaat tatcactcgt
     4321 acttgcaaca gtaggtaatg gattatgccc gcaggatgtc gattcactcc acatcgtttt
     4381 cattgatggt ccactggtaa atgccggtcc cgacatccag gcttgtaaag acactgcgta
     4441 tgtacccctc aacggttcag ttcaatttgc aggaggtgga atttggtcta gcagcggaac
     4501 aggaacgttt tctcccgcca atgatcagtt aaacgcaacc tacattccca gcaccgccga
     4561 tacactggca ggatctgttt ggctgtactt aactgctacc aacgtaggaa attgtatcac
     4621 cacaacggat agtctaaatc tgagttttta caatcctccg gtagcaacca ttctcaataa
     4681 caatgttgcc tgcagcggag atccgattcc acttcaggta aattcgacta caggacaagg
     4741 tgtttggaca actcttggca gcggattatt tcaacccgac agttctatta tttcatggta
     4801 ccagccagga gcaaacgata ttgcaacagg aacggtaaca ttgattttca cctctaccaa
     4861 caatggcggt tgcaaacccg cgcgcgacac gatcaatgta tccataattc catcacccgt
     4921 tcctgttttc acctttactg aaccatgttt tggtatagcc agtacattcg acgatgcctc
     4981 aactgcagtt ggaggagtaa cagcttggtc ctggaacttt ggagatggta gtccactatc
     5041 aacaacacaa gatccaacac atcttttcaa tacttccgga acgtttaatg taacactgat
     5101 tgtaacctca ggtaacggat gccgggatac gcttacacaa acagtcaatg tgcaccactt
     5161 accacagcct gcatttctaa cctcctaccc ttgtttgaat ggaggaacct catttcagga
     5221 tttatcttca gtaatgaatt caaccctcac aacatggtca tggacatttg gcgatggtgg
     5281 tgtatctgca aaccagaatc ctacgcacca atatcctgct tcaggcagtt attccgttac
     5341 gctaaccgta acctcagcat tgggatgtag tagttccgta acacaaaaca ctaacgttct
     5401 tccaggacca aacgcagcat tttcagtaag tgacaattcc gtaaaccaat ttgaaacggt
     5461 agtattcact gatcaatcca caccagcagg acaaatcacc ggttggttat gggattttgg
     5521 tgataattca ccacaggtaa acactcaaaa cagcagctac gcatacaatg gctccggcac
     5581 ctatattgta accctggttg taaccgataa taacggttgt atcgacacag cacgcaatac
     5641 agtaatcgtt ttccttcctc ctgatattcc aaatgcgttt acacctaatg gtgacggaag
     5701 taatgatttc cttcagattt tcgggggacc gttccgcgaa ttggaactga atatttacaa
     5761 caattgggga caggttattt acacaacaac cgatgcaaca gattgtggag gatatgtttg
     5821 tgttggatgg gatggaaaat tcaacggcat tgatcaaccc atgagtgttt atgtatacca
     5881 actccgggct gttactgaag atggtgtaga acatatttta accggag
//
LOCUS       DAOMYG010000173         5910 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4596_length_5910_cov_9.364475, whole genome shotgun sequence.
ACCESSION   DAOMYG010000173 DAOMYG010000000
VERSION     DAOMYG010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4596_length_5910_cov_9.364475"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PK610_06935"
     CDS             <1..335
                     /locus_tag="PK610_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35704.1"
                     /translation="IPTTISSTGDFRLVVEINGIIIGIESQGDPNTNLQNRLIDLADN
                     FHCEIILCSSRTRGDTVFAVDNLANTRGFQTIWTSTYQIANTANHNLVNRLKAKHLLD
                     LLQGLSLI"
     gene            1020..1460
                     /locus_tag="PK610_06940"
     CDS             1020..1460
                     /locus_tag="PK610_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRJ35705.1"
                     /translation="MAKTKTTYTGQDVTDFINSYVDNDLKKADSFQLIKLMQEWSDSE
                     PKMWGPSIIGFGNYHYKYASGHEGDAPVLAFSPRKAALTLYVYSDTEKSKKLLTDLGK
                     FKMSKACIYVKKLSDINTDNLREICIESIKYISEHHECSCRVKN"
     gene            1656..2306
                     /locus_tag="PK610_06945"
     CDS             1656..2306
                     /locus_tag="PK610_06945"
                     /inference="COORDINATES: protein motif:HMM:NF027591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4919 domain-containing protein"
                     /protein_id="HRJ35706.1"
                     /translation="MNKKLTISIVTLLISVSTFGQTETKIIVPDFKDKYSDYVRQLEA
                     GETDIDYQDFRFSFIESEQFIIANKNSTKLDSLKREMYAKMKTKSYSEIVDITKQMLS
                     IDYTSMIAHKILRQTYNIIGDTANSIKYKTIQFGLLNSIVKKGDGQTCATAWAVIQLS
                     EENFILEMLGAQLQKQSIDNDGGLCDKMEVKMDGKKKTYYFETSKVFLGYKKLGLE"
     gene            complement(2536..2772)
                     /locus_tag="PK610_06950"
     CDS             complement(2536..2772)
                     /locus_tag="PK610_06950"
                     /inference="COORDINATES: protein motif:HMM:NF019984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HRJ35707.1"
                     /translation="MSFEECIARINEVQRGWINYFQGTSIHGKLRDFDGWLRNRLRYC
                     IWHHWKKPERKRTLLRHSLRRVKEDNRLLHRAKF"
     gene            complement(3815..>5910)
                     /locus_tag="PK610_06955"
     CDS             complement(3815..>5910)
                     /locus_tag="PK610_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRJ35708.1"
                     /translation="QLDLKSLEFVRIDPALKYYSADSSLRIELDRKEAKDFRLYQGKN
                     KKIVPLTTAPKKYFSEDPLRAAIAPDAKTIVVIFRDRVHLFDRNTLKEKVIWIEDELK
                     YPMNDNWLDPIIEFSPTGNKLLLSANSNRPYYDAALLFLDLRSGKIEHLLKNKNAKSR
                     GQAEWLALSNDSTLKVYEKEIVYKKYSESPESYAEERLLCYECADYSIFKNFDERMYT
                     YGKGMEYRRNDSLIATFYLFQDLTYFVVLPNNYYFTNSLDLSQMIFRVNGRSYPFEQF
                     DLRYHRPDIVLKQLGYASPKLIEAYHQAYKKRLAKLGLEEQMLQDDFHLPEIRIDGFE
                     RLPVITSDTSIGLNLQLRDEKYEIDRIRVWVNDVAVYGQAGIPVHKKGTVQQKISVPL
                     TAGKNKIQIAVLNAAGVESLKETFVLQSNAAVKKPDLYMIAIGAGDFEQTQFNLRYPA
                     KDAADMVQLFSESKKYGQVFSKTLTGKEVTKTSILQLKQFLLQANVQDEVIVFFAGHG
                     VLDAQLDYYLATYDMNFLSPTQKGLAYSDLEGLLDGIRPQKKLLILDACHSGEIDKSG
                     AELSNETILYEKNEDLAFRNVGTATVKLGASASTELMRSLFLDLRKGTGATVISSAGG
                     AELSMEGSAWKNGLFTYCLLEGIRSAKADLNKDGQIQLSELQSYLALRVAALSNSMQQ
                     PTSRIVNKEVDFVVW"
BASE COUNT         1749 a         1346 c         1210 g         1605 t
ORIGIN      
        1 ctatacctac aaccatttca tctaccggag attttcgctt agttgtagaa attaatggaa
       61 taattatagg cattgaaagt caaggcgacc caaatacaaa tttacaaaat agacttattg
      121 accttgccga caattttcat tgtgaaatta ttttatgttc atcccgaaca agaggtgaca
      181 cagtttttgc ggttgacaat ttagctaata caagaggatt tcagactatt tggacttcaa
      241 catatcaaat tgcaaataca gcgaatcaca atcttgtaaa cagactaaag gcaaaacact
      301 tattagattt acttcaagga ctttcactaa tatgacagcg aacaaacaca aaaagagaga
      361 agaacgaacc gctaacagca cctacccaaa agtggcggtt cagtggttaa atcaagcttt
      421 gtgcttctat caaagtttct gcttggttga cagtgaagtg cttcgaaatc gccaccttcg
      481 ggtagctgca aaacgttagc ggtaatggta accggacgta aaaccaaatt gacaatgacc
      541 aaatttatat tgacattttt aatgatagca ttagtaacaa tgtctatcat ttttgcttgg
      601 acatttgtca acaggtggac aatgcccttt aactctgagg ggaattactt tgacttgaat
      661 accggagttt catatcatga acagtcagta atattttacg gagtaataac aacaattctt
      721 atgctgttta ccgctttgac aggcttttat ctgaaaagaa agtttacaca tcaagatggc
      781 aaaacaaaat cataaaatat gttcgagaga agccaaaatg aacaaaccac taccgctaac
      841 agcacatttg caattggcgg ggtttcgtgc tccgcagaca gtttagtggt agccgaaagt
      901 tttgttctcc gcatcaacat ttgtgctaaa aatcccgcca tcgccccccc aaaaaaacgt
      961 tagcggtcat tgtaaaaacg accacccaat cacgagtgac aactatttga ataaaaaata
     1021 tggcaaagac aaagacaaca tacacaggac aggacgttac cgactttata aactcttatg
     1081 ttgacaacga cctaaaaaag gctgacagct ttcaacttat taagctaatg caagagtggt
     1141 ctgactctga acctaaaatg tggggcccga gtattatcgg ttttggtaat tatcattata
     1201 aatacgcaag cggacacgaa ggagatgcac cagttcttgc attttcacca cgcaaagcag
     1261 ccttgacact ttatgtttac tctgacacag agaaaagtaa aaaactactg actgaccttg
     1321 gaaaatttaa aatgagtaaa gcctgtattt atgttaaaaa actttcagac atcaatactg
     1381 acaaccttag agaaatttgt attgaatcaa taaaatacat tagtgaacat cacgaatgtt
     1441 cttgtagagt aaaaaactaa caatgacaaa taaagataga aacactgctg gtaacacggg
     1501 ttttgcgtct ggctggctga agtgcaaacc tggagctttg tgcttctatt caagttcagt
     1561 gctggttgat cccgcacgtg cgggactccg aaacccgccc gaacgcaaag cccgaaaacg
     1621 ttatggctca ttttaaaaac gacacagcaa acattatgaa taagaaatta acgatttcta
     1681 tagtgacact tttaataagt gtttcaactt tcggacagac agaaaccaaa attattgttc
     1741 cagattttaa ggataagtat tctgactatg tgagacaatt agaagcagga gaaacagaca
     1801 ttgactacca agactttcgg ttcagtttca tagaaagtga acaatttatt attgcaaaca
     1861 agaactcgac aaaacttgac agcttaaaga gagaaatgta tgctaaaatg aagacaaaga
     1921 gttattctga aatagtcgac ataacaaagc aaatgctgag tattgactat acaagcatga
     1981 ttgctcataa aattttgcga cagacttata atattatcgg agacacagca aattcaataa
     2041 aatataagac tatacagttt ggacttctga attcaatagt caaaaaagga gacggacaaa
     2101 cttgtgcaac agcttgggca gtcatacaac tatcagaaga aaattttatc cttgaaatgt
     2161 taggtgcaca acttcaaaag caaagcatag acaatgacgg tggactttgt gacaagatgg
     2221 aagtcaaaat ggacggaaag aagaagactt attattttga aacgagcaag gtatttcttg
     2281 gctataaaaa acttggactt gaatagaatg acaagaaaaa caagccataa tcgagtagac
     2341 tgcctcactg aatttctcca gtgaggtgcg cccccgcctc tgcgagtcgg gtaaatctca
     2401 caccatcagc cgcggctgac ggtttcccga aaaaatttcg ggacaggctc caactatatg
     2461 ggttcaataa aatgtaaatg tccagtagcg attgataccc tcgtttcttt agccgagact
     2521 tagtgatcgt tgtgtttaga atttggctct gtgcaacaac ctattgtcct cttttaccct
     2581 tcgtagacta tggcgtagaa gggtcctctt tctctcaggt ttcttccagt gatgccatat
     2641 gcaatagcgc aggcggttgc gcaaccatcc gtcgaagtcg cggagtttgc catgaatgct
     2701 tgtcccttga aagtagttga tccaccctcg ctggacttca ttgatcctgg ctatgcactc
     2761 ctcgaaactc ataggcgtgg tttttcgggt gatggttttt agtcgttgct tgagtctttt
     2821 ccacactttc tctgttacta ccaattgata cttgcccttt actctattcc ggtagttgaa
     2881 ctaaaaaaag gaagggtcag cgacaacgta gtaggtcaaa ttcaacgtta catgggttat
     2941 gtcctagaag aacttgccga gaaaaatcaa actgttaagg gcgttatcat tggtctagaa
     3001 gacgatatca gaattaaaag agcattgtct gttacaaaca acattgaatt ctatcgatat
     3061 caggttaatt tcagattgtt taaggggtaa aggagaggaa tattctatca attcgtttcc
     3121 tccgaaaatt tccaccaacc gcaccacccg gattcctcca caaggtcgga atttgtacgc
     3181 gcagattcac acgaatcaac aaaagaaaga gacgaaacca ctcataacca ctaacccata
     3241 actcattact aaacccataa acaaacgcaa gggacggaac gatcgctcat acgctaattt
     3301 gcagattcgc taattttttt cctccgtgtc ctcttacgcc tccgcggttt aatttccctt
     3361 agtgaaactt agtacccttc gtgtctaagt ggtaaaaaat ccgagcccgc gagaaaaccc
     3421 tccgtgtcct tccaccacag gcggacaagc ttccttcctc tgcggtaaaa atccttagtg
     3481 tccattagtg ctcttagtgc cttccgccta cggtattcac tcttactttt cttagtgttc
     3541 ctaagtgtat ttagtgtcta agtggtgaga tactagaatc ctccggtgga caagcagtgg
     3601 ttccctcctc cgagcctgcg aggaaaactc tgtgtccttc caccacaggc ggacaagctt
     3661 ccttcctctg cggtaaaaat ccttagtgtc cattagtgct cttagtgcct tccgcctacg
     3721 gtattcactc ttacttttct tagtgttcct aagtgtcttt agtgtctaag tggtgagata
     3781 ctagaatcct ccggtggaca agtagtggtt cccctcacca aacaacaaaa tccacctcct
     3841 tattcacgat cctcgacgtc ggctgctgca tgctattcga aagcgccgcg acacgaagcg
     3901 ccagatagga ttgcaattcc gacaattgga tctgtccatc cttattgaga tctgcttttg
     3961 cggaacgaat accctcgagc aggcaataag taaacaatcc atttttccag gcgctaccct
     4021 ccatggaaag ctctgcacca cctgcgctgg agataacggt ggcaccagta cccttgcgga
     4081 ggtcaaggaa gagcgatcgc attaattccg tgctggcaga agctcccagc ttaacagtgg
     4141 ccgtacccac attgcggaag gcgagatcct cgttcttttc gtagagaatg gtttcgttgc
     4201 tgagttcggc gccgctctta tcgatctcac cgctatggca ggcatcgagg atgagcagtt
     4261 ttttctgggg acgaatacca tcgagcaatc cctcgagatc ggaataggcc aatccctttt
     4321 gcgttgggga tagaaaattc atgtcgtagg tagcgaggta ataatccagc tgcgcatcca
     4381 gcacaccatg acccgcaaag aagacgatca cttcatcctg cacattggcc tgcagcagga
     4441 attgtttcaa ctggagaatg gaagtcttgg tgacctcctt gcccgtgagt gttttcgaaa
     4501 agacctgtcc gtatttttta ctctccgaaa aaagctgcac catatccgcc gcatccttgg
     4561 ccgggtaacg gagattgaac tgcgtctgtt cgaagtcacc cgccccaatg gcgatcatat
     4621 acagatccgg ttttttgacc gccgcattcg attgcagcac gaaagtttcc ttgagactct
     4681 ccacccctgc tgcattgagc accgcgatct ggatcttatt ctttcccgct gtgagcggca
     4741 ccgatatttt ttgctgcacc gtcccttttt tgtgcaccgg gatacccgct tgtccgtaca
     4801 ccgccacatc attcacccac acacgaatgc gatcgatctc atacttctca tcacgcaatt
     4861 gcagattcaa tccgatcgag gtatcagaag tgatcaccgg caaacgctcg aaaccatcga
     4921 tcctgatctc cggaagatgg aaatcgtcct gcaacatctg ttcctccaat cccagtttag
     4981 ccaaccgttt tttatacgcc tggtgatagg cctcaataag tttaggtgat gcatagccca
     5041 gttgtttcaa cacgatatcc ggacgatgat aacgcagatc gaattgctcg aaaggatagg
     5101 atcggccatt cacccggaag atcatctgac taagatcaag gctattggtg aaataataat
     5161 tattcggcag caccacaaaa taggtaagat cctggaagag atagaacgta gcgatcagtg
     5221 aatcgttgcg cctgtactcc attcccttgc cataggtgta catgcgctca tcgaagtttt
     5281 tgaaaatgga atagtcggca cactcgtaac aaagcaagcg ctcctccgca taggattccg
     5341 gcgattcgct gtatttttta tagacgatct ccttctcata caccttgagc gtgctatcat
     5401 tgctcagcgc caaccattct gcttgtccac gacttttcgc atttttattt ttgagcagat
     5461 gttcaatttt cccactgcga agatccagga agagcagcgc agcatcgtag tagggtcggt
     5521 tggaatttgc agaaagcagt agcttatttc ccgttgggga gaactcaatg atcggatcca
     5581 gccagttatc gttcatcgga tatttcagtt catcttcgat ccagatcact ttttccttga
     5641 gcgtattccg atcgaagaga tgcacccgat ccctgaagat caccacgatc gtttttgcat
     5701 ccggagcaat cgccgcccgc aaaggatctt cactgaaata tttttttgga gcggtagtca
     5761 gcggaacgat ctttttgttt tttccctgat acaagcgaaa gtccttcgct tccttccgat
     5821 ccagttcaat acgcaaggaa gaatccgccg aataatattt caaagcagga tcgatgcgga
     5881 caaattcaag acttttgaga tcgagttggt
//
LOCUS       DAOMYG010000174         5887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4625_length_5887_cov_9.076303, whole genome shotgun sequence.
ACCESSION   DAOMYG010000174 DAOMYG010000000
VERSION     DAOMYG010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4625_length_5887_cov_9.076303"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..303
                     /locus_tag="PK610_06960"
     CDS             142..303
                     /locus_tag="PK610_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35709.1"
                     /translation="MSHDNHGHDHGHDHGHDNHGSEHIFFDGGGVAGVSWIYMLAALG
                     ILAYMVFAG"
     gene            complement(358..2235)
                     /gene="asnB"
                     /locus_tag="PK610_06965"
     CDS             complement(358..2235)
                     /gene="asnB"
                     /locus_tag="PK610_06965"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRJ35710.1"
                     /translation="MCGINGIFGIEKIGSPDTVIGKMNDAIAHRGPDAAGVWKNDQLA
                     LGHRRLSIIDLSPAGNQPFISADGKTVIVFNGEIYNYRELKTQLGDFNFRTNSDTEVL
                     LAAWFKWGKECVHRLDGMFAFAIWDNDKQELFIARDRMGVKPLYYSQSGNSFLFSSEL
                     RGLLASGLVDRALDEEALTDYLKYQTVHAPTTLLRNVKMLLPGSCMVVTDTEFSIYSY
                     WDAASNTNYDSKGREYTEVVGEVRALLDASVKKRLIADVPFGAFLSGGIDSSAVVGLM
                     AQHSSQPVKTFHISFGEKEFSEAEYARKVADRFSTNHTEILIHPDEFLRDLPAALNAL
                     DHPSGDGPNTWVVSRATKQAGVTMALSGIGGDELFAGYDVFRQLHGLSSKQWMQYFPM
                     LLRRPAGSALMALRKGVRSDKLDEFLRLEYFELEYVYPLSRQVLKNKKTAALLGRKQL
                     PVSAVKKILDAGIGSGTAGAGLPGLSRISYAEMYTYLQNVLLRDTDQMSMAHALEVRE
                     PFLDHQLISYVMGIRDSYKFPDRPKKLLVDAMGDLLPKAIWDRPKMGFTFPWKHWMKN
                     ELRDFCEERINYLSQSPYFNAKELTLLWNAFLNDDPRYPWSRIWHLVVLGHWMQKNAM
                     N"
     gene            complement(2405..3520)
                     /locus_tag="PK610_06970"
     CDS             complement(2405..3520)
                     /locus_tag="PK610_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010522975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRJ35711.1"
                     /translation="MRRKSRYIYDLKQWPDFNWRTEEIVSLLSEVRYLQGRILGRMDA
                     LGFDLRNEAAFDTMTLDVIKTSEIEGEILDRDQVRSSLARRLGLDIPGEVNSDRNVDG
                     MVEMMLDASINCFQPLKEDRLFAWHATLFPLGRSGMYPITTANWRTDANGPMQVVSGA
                     LGREKIHFRAPDATLVKTEMRAFIRWFNKKNPEVDLVLKAAIAHLWFVTVHPFEDGNG
                     RIARALTDMLLAQSDRSTQRFYSMSAQIRLERKKYYEILERTQSGTMDVTEWIKWFLQ
                     CLIRAIQSTESQLNRVLFKADFWSKNATTVMNERQKKMMNKLLDGFEGKLNSSKWARM
                     MKCSNDTAIRDINDLIEKGILEKEDAGGRSTSYRLVC"
     gene            complement(3951..5012)
                     /locus_tag="PK610_06975"
     CDS             complement(3951..5012)
                     /locus_tag="PK610_06975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ35712.1"
                     /translation="MENKRIVFLYTEIAGYILSCISELLRHGNYEVTLVRWPLNKEAP
                     FDFKFPEGLKVEDRTQFNDDALMSRVDSIDPHFIFCSGWVDKGYLKVCKKYRDRIPVV
                     VGIDNQWEGNWRQRMASLLSFRMIKPYFNAAWVPGEKQREFAGKLGFPSDKIYTGFYS
                     ADTAHFFNYYNRFKAQKEQEFPRRFIYVGRYLSFKGVNELWDAFAELQQENPSDWELW
                     CLGTGDLWEKRKEHDKIKHFGFVQPGEIENFIRDTGVFILPSTFEPWGVVIHEFAASG
                     FPIICTNKVGAASRFVQDGKNGFVVSPSDKNALKTAMKKMMQCSPDELNKMGEASHEL
                     ANGLTPKVWVETLGSMLMC"
     gene            complement(5002..>5887)
                     /locus_tag="PK610_06980"
     CDS             complement(5002..>5887)
                     /locus_tag="PK610_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35713.1"
                     /translation="QYYSTGLPAKTVLSNIEAINRDQPLFLAHLGDFFLDVRNDIPAY
                     TENLLNRFEFPVYNAVGNHDLSGNVYQENFGATWFAFNVGIDRFIFLDTEIADGSIRD
                     EQQSFLLRSLDSLPEKARVFIFAHRLIWAEDHPDLKNLFTDNTRSANPGNFRVAILPE
                     LKKIAEHHPVVFAGGSMGNVPSSFFFHKEKEADITYIATAIRDTPRDALLRVEVSGSG
                     IHYTAFHLPLSPEAYDLSYYRPEQKTKSPFNWRLVPLYTTQAVSHRYFWYGVLFMVVP
                     IALVVGMMMRRKKRKNGK"
BASE COUNT         1705 a         1443 c         1133 g         1606 t
ORIGIN      
        1 tatttgaggg agtgacctct tttcgccttc atttactatt tgtgtttata aatcaacgaa
       61 ttacaaattt attttgggta cagatttttt tatccaccta aaggaaaatt cctatttttg
      121 aatcaacaaa attgaactac catgagccac gacaatcacg gacacgacca cgggcatgac
      181 cacggtcacg ataaccacgg aagcgagcac attttctttg atggaggtgg tgtagctgga
      241 gttagctgga tttatatgct ggctgcactg ggcattcttg cctacatggt atttgcagga
      301 taaaatttat tcattgtaaa tagtaaaggg aacacgttgt gttccctttt tttgtttcta
      361 attcattgca ttcttctgca tccaatgccc caacaccacc aggtgccaga ttctcgacca
      421 agggtagcgc ggatcatcat tgagaaaggc attccagagc aatgtgagtt cctttgcgtt
      481 gaaataaggg ctttgtgaaa gataatttat acgctcttca cagaagtcac gcaattcgtt
      541 tttcatccag tgtttccagg ggaaggtgaa tcccattttg gggcggtccc agattgcttt
      601 gggaaggaga tcacccatag catcaaccaa tagttttttc ggacgatccg gaaatttgta
      661 actgtcgcga atccccataa cgtaggagat gagctgatga tcgagaaaag gttcacgcac
      721 ttcgagtgca tgcgccatac tcatctgatc ggtatcacgc agcaaaacat tttgcagata
      781 ggtgtacatt tctgcatagg aaatacgact caagcccggc aatcctgcac ctgctgttcc
      841 gcttccaatt ccggcatcca gaattttttt cactgcgctt accggcaatt gttttcgtcc
      901 caggagtgct gctgtttttt tattcttcag cacctgtcgc gaaagcggat aaacatactc
      961 cagctcaaaa tattcgagac gcaaaaattc atccagctta tccgaacgaa cacccttacg
     1021 caatgccatc aatgcacttc ccgcagggcg acgaagtaac atgggaaaat actgcatcca
     1081 ctgtttggaa gagagaccgt gcagctgacg aaaaacatcg tatcccgcaa acaactcatc
     1141 acccccaata cctgataaag ccatggtgac tcctgcttgt tttgtagcac gcgataccac
     1201 ccaggtattc ggaccatcac ccgaaggatg gtcaagcgca ttgagcgcag cgggcagatc
     1261 gcgaagaaat tcatccggat gaatgaggat ctcagtatgg ttggtagaaa accgatcagc
     1321 aactttacgt gcatattctg cttcgctgaa ttccttttct ccaaacgaaa tatgaaaggt
     1381 ttttacaggc tgcgaagaat gctgagccat caacccaaca accgcagagg aatcaattcc
     1441 ccctgaaaga aatgccccaa aaggaacatc agcaataagt cgttttttta cagaagcatc
     1501 cagcaacgca cgaacctctc caacaacttc agtgtactct cttcctttac tatcgtaatt
     1561 tgtgttagac gctgcatccc aataagaata tatagaaaat tccgtatcag taactaccat
     1621 gcacgaaccc ggtaagagca tttttacatt ccgaagtaac gttgttggag cgtgaaccgt
     1681 ttggtacttc aggtaatcgg tcaatgcctc ttcatccagg gcgcgatcca ccaatccact
     1741 cgccagtaat ccacgcagct cgctcgaaaa gaggaatgaa tttccacttt gtgaataata
     1801 caaaggcttc acccccatgc gatcacgtgc gatgaataat tcctgtttgt cattgtccca
     1861 gattgcaaag gcgaacatcc catcgagacg atgaacacat tcttttcccc atttgaacca
     1921 agctgcgagc aatacttctg tatcggaatt ggtgcgaaaa ttaaaatctc ccagttgggt
     1981 ttttaactcc cggtaattat aaatctctcc attaaaaaca atcaccgtct ttccatctgc
     2041 cgaaataaaa ggctgattcc cggctggaga aagatcgata atagaaagtc gacgatgtcc
     2101 gagcgccaat tgatcgttct tccacacccc tgcagcatcc ggaccacgat gtgcaatggc
     2161 atcgttcatt ttcccgataa ccgtatcagg tgaacctatc ttttcaattc cgaatattcc
     2221 gtttattccg cacatattat aattttttca tcctgaagtg aaatcgaatg caatgagatt
     2281 tctacttcag gaccaattta ccaattgcca aattaaagaa ccggaacgag atcaggacga
     2341 ggacgaagaa agaagacgct ctcctctcct agcatcagat ttaacaggcc agcacaccag
     2401 caatttagca tactaaccta taactcgtac tccttccacc cgcatcttct ttttccaaga
     2461 tacctttttc aatcaaatca ttgatgtccc tgatcgctgt atcgttcgaa cacttcatca
     2521 ttcttgccca tttggaggaa tttagttttc cttcaaatcc atccagtaat ttattcatca
     2581 tttttttctg ccgctcattc attactgtcg ttgcgttttt actccagaaa tcagccttga
     2641 ataaaacacg attcagttga ctctcggtcg attgaatggc acgaatgagg cactgcaaaa
     2701 accattttat ccactccgtt acatccattg ttccggattg cgttcgttcc aaaatctcat
     2761 aatacttttt gcgttccaaa cgaatctgtg cagacatact gtaaaaacgc tgcgtgctcc
     2821 gatcagattg agcgaggagc atatctgtta gcgcacgtgc tatacgacca ttcccatcct
     2881 caaagggatg caccgtcaca aaccacaaat gagcaattgc agccttcagc accagatcaa
     2941 cttcaggatt tttcttattg aaccaacgaa taaatgcgcg catttcggtt tttaccagtg
     3001 tagcatccgg cgcgcggaaa tggatctttt ctcgtcctaa tgctcctgaa actacctgca
     3061 tcggtccatt agcatcggtt ctccaatttg cggttgtaat gggatacatt ccgcttcgac
     3121 ccagtgggaa aagtgtagca tgccaggcaa acaagcgatc ttctttcagt ggctgaaaac
     3181 agttgatcga ggcatccagc atcatctcaa ccattccatc cacgtttcgg tcagaattta
     3241 cctcgcccgg aatgtccaaa cccaacctgc gtgcaagcga tgaacgcact tggtcgcgat
     3301 ccaaaatttc tccttcaatt tcactggtct tgataacgtc tagcgtcatc gtgtcgaatg
     3361 ctgcttcatt gcgtaaatca aatcccaagg catccatccg acccaaaatg cgcccctgca
     3421 aatacctgac ttcactcaat agcgaaacga tctcctccgt tcgccaattg aaatcaggcc
     3481 attgttttaa atcatagata taacgagatt ttcgacgcat attctgcgac aaatataaag
     3541 gatattcgcc gcaaacacaa attaatcacc gcaaatttag cgacgattag gcttcgtatt
     3601 caccgtaaac cggtaaatca actgttatat tgcatgctcc ccgggcggaa caatgggtgg
     3661 agaacgatcc tcctacattg tgcttcggcg gacaagggac gaggaaagaa gacggggacc
     3721 agggatgaag acgaatgtac gagtgagatg aatctgcctt cactgtgtta cggcggacaa
     3781 gtgtgcgaat gaattctgct tcacgatgaa ggttttattc taagagggtt tttaaaattt
     3841 cgatttcttt cgaagaacaa tctcaaatgg tacaaggatt cgttcgcaca ttgttcgacc
     3901 ggaagaagaa caaccctgac cgtaagcgtc aggattccca cattcacaca ttagcacatt
     3961 agcatactac ccaacgtttc cacccaaact ttcggagtta atccattggc cagttcatga
     4021 cttgcctcac ccattttgtt tagttcatca ggagagcatt gcatcatttt tttcattgct
     4081 gtcttcaatg cgttcttatc ggaaggtgaa accacaaatc cgttttttcc gtcttgcaca
     4141 aaacgagaag cagcgccaac cttattggtg caaataatag ggaatccgct tgcagcaaat
     4201 tcgtgaatga ctactcccca aggctcaaaa gtgctcggaa gaataaatac tccggtatcg
     4261 cgaataaaat tttcgatctc tccgggttgt acaaaaccaa agtgtttgat cttatcgtgc
     4321 tccttacgtt tctcccacaa atctcccgta cccaaacacc ataattccca atcagaggga
     4381 ttctcctgct gcaattcagc aaatgcatcc cataattcat ttacgccttt aaacgaaaga
     4441 tatcgtccca cataaataaa cctcctgggg aattcctgtt ccttttgcgc tttaaagcga
     4501 ttgtaataat taaaaaaatg agcggtatca gccgaataaa atccggtgta aattttgtcg
     4561 gaaggaaatc ccagtttacc tgcaaactcc cgttgttttt ccccgggcac ccaggctgca
     4621 ttgaagtacg gtttgatcat tcgaaacgac aagagggatg ccatacgctg cctccaattt
     4681 ccttcccact gattatcgat gcccactacc acaggaattc ggtctctgta ttttttgcac
     4741 actttcagat agcctttatc tacccaacca ctgcaaaaaa taaagtgcgg atcgatcgaa
     4801 tctaccctac tcattaatgc atcatcattg aactgagtcc ggtcttcgac ctttaatcct
     4861 tccggaaatt taaaatcaaa tggcgcttcc ttattcaatg gccaacgcac caaagtaact
     4921 tcatagtttc cgtgccgcaa caattcactg atacaggata aaatatatcc tgcgatttcg
     4981 gtataaagaa atacgatgcg cttattttcc attttttctt ttcttcctgc gcatcatcat
     5041 tccaacaaca agcgcaatgg ggaccaccat aaacaaaacc ccataccaga aatacctgtg
     5101 cgatacagcc tgggtagtat aaagcggtac cagtcgccag ttgaaaggcg atttcgtttt
     5161 ttgttcagga cggtaatacg aaagatcata cgcttccggc gacaatggca aatgaaaagc
     5221 agtatagtgt attcccgaac cactcacttc aaccctcaaa agtgcatcgc gcggtgtatc
     5281 gcgaatggct gttgcgatat aggtaatatc tgcctccttt tccttgtgaa agaaaaacga
     5341 agaaggcaca ttgcccattg atcctcctgc gaaaacaaca ggatgatgtt ctgcgatctt
     5401 ttttaactcc ggcaaaatag caaccctgaa attaccggga tttgcggatc gtgtattatc
     5461 ggtaaacaga ttttttaaat ccggatgatc ttctgcccag atcaaacggt gcgcaaaaat
     5521 aaaaacacgt gctttctccg gtaatgaatc aagtgatctc agtaaaaatg attgctgttc
     5581 atcgcgaatg ctgccatctg caatttctgt atcgagaaaa ataaaacgat cgatccccac
     5641 attaaaggca aaccaggtag caccaaaatt ctcctgatac acattgcccg ataaatcatg
     5701 attacctacc gcattataaa ccggaaattc aaaacgattc agaagatttt cggtataagc
     5761 aggaatatca tttcggacat ccagaaaaaa atcacccaag tgcgcgagaa acaagggttg
     5821 atcgcgattt attgcctcaa tattactcaa cacagttttt gcgggcagac ctgttgagta
     5881 atattga
//
LOCUS       DAOMYG010000175         5871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4647_length_5871_cov_9.099897, whole genome shotgun sequence.
ACCESSION   DAOMYG010000175 DAOMYG010000000
VERSION     DAOMYG010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4647_length_5871_cov_9.099897"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>5871
                     /locus_tag="PK610_06985"
     CDS             <1..>5871
                     /locus_tag="PK610_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35714.1"
                     /translation="MSRQQIAKNNTDSLAIVQVTYYDHHGRAALSSLPAPSTQPNLKY
                     RFRWNRPSDPGLSNISYSKEYFDKEQTECVVSPSAMSSAFGAGNYYSTSNNYTTGFQG
                     YVPDAEGYPFVQIQYMPDGTGRASKIGSAGPEFQINDPHSVSMEYGSATQQRLNQLFG
                     TDAGYEVQYQKNTIVDGNGQRVVSYLDGSGRVVATALAGEAPSNVMTLNTNTVTNVQT
                     PIIEPGDDAQSSSTPGELNFESVISVDFEGEHIFSYGISPGTMANECHPELCMDCVYD
                     LEFTITDVLCGQVIYTHSKTINGAALDNSCNGLALDTIGTSVIAVDLVPGSYQISKKL
                     KVNQASLEYYLAQYLELNTCLLDYSDFLEQALAEANTSGCNSSPCILSCIQSLGTREA
                     YIALGNTGAQWDVLFAACLNDCEATSTAYCDNFRQSMLDDVKPNGQYGLVTFSAGTYS
                     STDPLSVYYVSNDLPNTNAYWRNPVGGYKNSDGSTSYILNSSNVLVTPEHASITLTEF
                     INAFQDSWADALLPYHPEYCYLSFCESQEASHQSDMLMLGVQTYDEAKALGYLNPLLM
                     NSGQGVPANAQSASTFVDPFYTNNPSIQSQFVNEMRNYHSFNGVNYTIWQMAWFLTYC
                     NGDTTVSGMTSCLTGFNEFGTNTCTKDLLWETFRDLYLELKANIYSDLQARYAIENKC
                     YSGCFGTTAFNDDAHDFDCYNASISPTCSVTKYDADQDPLQPCSDFTVSLYSGKIPIF
                     ADRRQSPYYGLNSGALSTLVNGQISTFQGDFCETMCEQKIEEWMQDLSECGLTTPQYN
                     AIKAEFLELCELGCDAQHASGASSLPTGVYTTGGNQSIHEILIDVLGSGYETAVCSEL
                     LINDVQPYGVVPDFQETYKLDTCGCDKVLYIKQLFLSGQPLPPGITSMDQLFAYTYGT
                     PLTGVKNFACRCESAWTAGYPSTPYHPSLPWLSTSATYLSTTTPFLYIPSGVACPSCV
                     NCETINGLVSSFHTRFTGLSSTHSNYGTLLATYINNALGYQLSSDEYLDFIEACGQES
                     NVPSCTPTQELHDLQNLLYSLSTLPSGGFLTTSSNPVLMSNLSIWAGSTLAGYTAGST
                     EQYWTNFGTNGANPDQSPLTLYIGNGSPCTIVLTLASGSTNRWSKLSDIKNLTFVQNG
                     CTSSYQFSATATFSTPGGWVTETIFGTTSCYPMATCVCGENLKLCNEFNFVPTIDPCV
                     QTVLNTAEANAQSAYEEYMEGIIAEFTANYLSVCIGAAANSEYLNWSYPSNEYHYTLY
                     YYDQAGQLVRTVPPKGVDGTFTASGVDANRPSGSRIAPSHTLTTEYRYNSFGQVVEQK
                     TPDGGVSKFWYDKFGRIAVSQNAKQEPGYYYSYTRYDIYGRPFEVGQVQKSSITQISE
                     AIVKNPSSLDAWIISGTRTEVTRTTYDRSISVSVVASKFTNGQNHLRNRISTVAHYFS
                     YSTITPDLSFNHATHFSYDDHGNVTEMIQDMPDLGPIQHQYKKIQYVFEQISGNVKKV
                     LYQKGSADQFLHEYFYDADNRIKEVYTSRDSTIWDRDAKYFYYRHGPLARVEHGHNKV
                     QGEDFAYTINGWIKGVNSGTLNTTYDMGKDGVTGYLSGNTNVHNRVAHDKFGYILGYF
                     NQDYKPVSGATPGNYFEPAYSGTGFGNASPSLYNGNIRHMITAIPTASNQVMGYAYQY
                     DQLNRIKSMNAFRFVSGNSWSTGTSTTDYQTTYKYDANGNIEELKRRAVGLLMDDLAY
                     SYVPNTNRLDKVDDLAGATPTISDDIEGIQSNGNYTYTAIGELKSDAQENIADITWRV
                     DGKVRRVTRNIAGKVEMEFEYDAFGRRVLKIEKLTTTGGSPVGQDQWTYTYYVNDASG
                     NVMATYKLTKGVTTETFKLDNQHIYGSKRLGMLVRDYTLTTRNVSTGINTHSAAEARN
                     HRSGRRYYEYSNHLGNVLLVTSDRKIPYYSGSTFLRYEPQMISFSDYYPFGSIMPGR"
BASE COUNT         1672 a         1389 c         1243 g         1567 t
ORIGIN      
        1 atgagtcgac aacaaatagc gaaaaataac actgattcac ttgcaattgt tcaggttacc
       61 tattatgatc atcacggtag ggcggcatta agctcattgc ctgcaccttc aactcaaccc
      121 aatctgaaat accgattcag atggaacaga ccttctgatc cgggattgtc aaatattagc
      181 tattccaaag agtatttcga caaggaacaa accgaatgtg tggttagtcc aagtgcaatg
      241 agttcggcct ttggtgccgg aaattactat tctacatcta ataattatac caccggattt
      301 cagggttatg taccggatgc agaaggatat ccattcgtac aaatacaata catgccggat
      361 ggaacaggac gagccagcaa aattggttct gcaggaccgg aatttcagat caacgatccg
      421 cattctgtat caatggaata cggaagcgca actcaacagc gactgaacca actttttgga
      481 acagatgcag gctatgaagt gcaataccaa aaaaacacta ttgttgacgg aaacggacaa
      541 cgggttgtat cttatcttga tggaagcggt agggttgtgg caacagcatt agcaggagaa
      601 gcgccttcta atgtgatgac ccttaacacc aataccgtaa ccaatgttca aacacctata
      661 attgaacctg gagatgatgc tcaaagttca agcactccgg gtgaattgaa ttttgaatcg
      721 gtaatatcag tggattttga aggtgaacac attttctctt atggcatttc accgggaaca
      781 atggccaatg aatgtcaccc cgaattgtgc atggattgtg tgtacgattt ggagtttacg
      841 ataactgatg ttctatgcgg acaagtcatt tacacccatt caaaaacgat aaatggtgct
      901 gcccttgata attcatgcaa tggattggca ttagatacca ttgggacatc ggtaattgca
      961 gttgatttgg tgcccgggtc gtaccagatt tccaaaaaac ttaaagtaaa ccaggcatcg
     1021 ctggagtatt atcttgcgca atacctggaa ctgaatacct gtcttctcga ttattcggat
     1081 tttcttgaac aggctttggc agaagccaat acttcaggtt gtaattcttc accttgcatc
     1141 ctctcctgca ttcaaagtct tggaacaaga gaagcttata ttgcattagg aaataccggc
     1201 gcacaatggg atgttctttt cgctgcctgt cttaacgatt gtgaggcaac ttcaacagcc
     1261 tattgtgata atttccgcca atcgatgctc gatgatgtta aacccaacgg gcaatacgga
     1321 ttagtcacct tttctgcagg tacctattct tctactgatc cgctttccgt ttattacgta
     1381 agcaacgacc ttcccaacac gaatgcttac tggagaaatc ctgttggtgg atataaaaac
     1441 agtgatggtt caacctccta cattctaaat tcatccaatg ttttggtaac tcctgagcat
     1501 gcatcaatta ctttaaccga attcatcaat gcatttcagg attcctgggc agatgcatta
     1561 ttgccttatc accctgaata ttgctacctg agtttttgtg aatcgcagga agcctcccat
     1621 caatccgata tgctgatgct tggggttcaa acctatgatg aggcaaaagc attgggctat
     1681 ctcaatcctc tgcttatgaa ttccggtcag ggagttccag ccaatgccca atcggctagc
     1741 acatttgtgg atcctttcta caccaataat ccaagtattc aatctcagtt tgtaaatgag
     1801 atgcgaaatt accattcctt caatggagta aattacacca tttggcaaat ggcatggttc
     1861 ctgacgtatt gcaatggcga tacaacagtt tcaggaatga cttcctgcct caccgggttc
     1921 aatgagtttg gaacgaacac ctgtacaaaa gatcttttat gggagacatt tcgtgatttg
     1981 tatcttgaat taaaggcgaa catttacagc gatctccagg cacgctatgc tatcgaaaat
     2041 aaatgttata gcggatgttt tggtacaaca gcatttaatg atgacgccca cgattttgac
     2101 tgctacaatg caagcatcag tccaacttgc agtgtaacca aatacgatgc cgatcaggat
     2161 cctttacaac cttgctctga ttttacagtg tcattgtact ccggtaaaat accaatattt
     2221 gctgatcgcc gacaaagccc ttactacgga ttaaattccg gtgctttgtc taccctggtg
     2281 aatggccaga tttctacttt tcaaggagac ttctgcgaaa cgatgtgcga gcaaaagatc
     2341 gaagaatgga tgcaggatct ttcagaatgc ggattaacca ctccacaata taatgccatt
     2401 aaggccgaat tccttgaatt gtgcgaattg ggttgtgatg cccaacacgc ttcaggagcg
     2461 tcttccttac ccacaggagt atatactaca ggaggaaacc aaagcatcca cgaaattctc
     2521 atcgatgtat tgggatcagg ttatgaaact gctgtttgct cagaacttct gatcaatgat
     2581 gtgcagccct atggcgttgt gcccgatttt caggaaacct ataaactgga cacctgtgga
     2641 tgtgataagg ttctttacat caagcaactt tttctctccg gacaaccatt gcctcccgga
     2701 atcacttcca tggatcagtt gtttgcctat acttacggaa ctccactaac cggtgtgaaa
     2761 aattttgcct gccgttgtga aagtgcatgg acagccggtt atccttccac tccgtatcat
     2821 ccatcattgc cctggttgag cacttctgcc acttacctga gtactaccac accttttctg
     2881 tatatcccat caggcgttgc ttgtccttct tgtgtgaatt gtgagaccat aaatggattg
     2941 gtcagcagtt tccacacgcg gttcacagga ctaagttcta cgcattccaa ctacggaacc
     3001 ttacttgcca cctatatcaa taacgcattg ggttatcaac tttcttctga tgaatacctt
     3061 gattttattg aagcatgcgg acaggaaagc aatgttccat cctgtactcc aactcaagag
     3121 ctccacgatc tacagaattt actctatagc ctctctacat tgccttccgg tggcttcctg
     3181 actacctctt ccaatcctgt attgatgagc aatctttcca tttgggcggg atccacgctt
     3241 gcaggttata ctgcaggatc gactgagcaa tactggacca actttggaac caatggagca
     3301 aatccggacc agtcgccact tactctatac attggaaacg gatcaccctg caccatcgtt
     3361 cttaccctgg caagcggatc taccaacaga tggagcaagc tctctgatat taagaacctg
     3421 acatttgttc agaacggttg tacttccagc taccaattct cagctactgc tactttctcc
     3481 actccaggtg gatgggtcac tgaaaccatt ttcggaacaa catcctgcta cccgatggca
     3541 acttgtgtct gcggcgaaaa tctgaagctg tgcaatgagt tcaattttgt tccgaccatc
     3601 gatccatgtg tgcagactgt tcttaataca gctgaggcca atgcccaaag cgcttacgaa
     3661 gaatacatgg aaggaatcat tgccgaattc actgccaatt acctttcggt atgcattggg
     3721 gcagcagcca attcggagta cctcaattgg tcatacccct ccaacgaata tcattataca
     3781 ctgtactatt acgatcaggc cggacagctg gtgcgtaccg tgccacccaa aggtgttgat
     3841 ggaacattta cagcaagcgg tgtagatgcc aatcgtccat caggatctcg aattgctcct
     3901 tcgcataccc ttaccactga ataccgctac aatagctttg gtcaggtggt ggaacaaaaa
     3961 acacccgatg gcggagtgag taaattctgg tacgataaat tcggacgcat tgccgtttcc
     4021 caaaacgcca aacaggaacc cggctattat tactcctata cccgctacga tatttacgga
     4081 cgcccctttg aagtaggaca ggtgcagaaa tcgtccatca cccaaatttc agaagccatc
     4141 gtaaaaaatc caagctccct tgatgcctgg atcatctccg gaacccgcac cgaagttacc
     4201 cgcactactt acgaccgatc catcagcgtg agtgtggttg catcgaaatt taccaatgga
     4261 caaaatcatt tgcgcaaccg catttctacc gttgcacatt atttttccta ttccacaatt
     4321 actcccgatc tcagtttcaa ccatgccacg catttcagtt acgatgatca cggaaatgta
     4381 accgagatga ttcaggatat gcctgatctg ggtcctatcc agcatcagta caaaaagatc
     4441 cagtatgtct ttgagcaaat ttcgggcaat gtgaaaaaag tgctgtacca aaaaggcagt
     4501 gccgatcagt tcctgcacga gtatttttac gatgccgata accgcatcaa ggaagtgtac
     4561 acctcacgcg acagtaccat ctgggaccgc gatgccaagt atttttacta ccgacacggt
     4621 ccactggctc gtgtggagca tgggcacaac aaagtacagg gagaagattt tgcctatacc
     4681 atcaacggct ggataaaggg cgttaactcc ggtacactga ataccactta cgatatgggt
     4741 aaagacggtg taacaggata cctgagcgga aacaccaatg tgcacaatcg agtagcacac
     4801 gataagtttg gttatattct tggatacttt aaccaggatt acaaacctgt ttcaggcgca
     4861 acaccgggca attattttga gcctgcctat tcgggaaccg gctttggcaa tgccagtccg
     4921 agcttgtaca acgggaacat ccggcacatg attacggcta tacctactgc cagcaatcaa
     4981 gtgatggggt atgcctacca atacgatcag ctcaaccgca tcaagagcat gaatgccttc
     5041 cgttttgttt cgggaaattc atggtccacc ggtacatcca ctaccgatta ccagactact
     5101 tataagtatg atgccaacgg gaatattgag gaattaaaaa gaagggccgt tggtctgcta
     5161 atggatgatc tggcttattc ttatgttcca aataccaata gattggataa ggtagatgat
     5221 ttggctggtg ccactccaac aatttcggat gacattgaag gaatacaaag caacggaaac
     5281 tacacctata ccgccatagg cgagttaaaa tctgatgctc aggaaaacat tgccgacatt
     5341 acctggaggg tagatggaaa agtgcgcagg gtaacccgca atatagcggg caaagtagag
     5401 atggaatttg agtacgatgc ctttgggcgc agggtattga aaattgaaaa actcaccacc
     5461 accggaggat ctccagtggg acaagatcaa tggacctaca cgtattatgt gaatgatgcc
     5521 tccggaaatg taatggcgac ctataaacta accaagggcg taactacgga gacctttaag
     5581 ctcgacaacc aacacattta cggctctaaa cgcctgggaa tgctggttcg cgactacacc
     5641 cttaccaccc ggaacgtatc taccggaata aacacgcaca gcgccgctga agcccgcaat
     5701 caccgttcag gacggaggta ttacgagtac agcaatcacc tgggcaacgt attgcttgta
     5761 acctccgatc gaaaaattcc gtattatagc ggttcaacat tcttacgcta cgaaccacag
     5821 atgatcagtt tctcagatta ttatcccttt gggagtataa tgcctggtag g
//
LOCUS       DAOMYG010000176         5836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4681_length_5836_cov_9.183532, whole genome shotgun sequence.
ACCESSION   DAOMYG010000176 DAOMYG010000000
VERSION     DAOMYG010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4681_length_5836_cov_9.183532"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PK610_06990"
     CDS             complement(<1..345)
                     /locus_tag="PK610_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35715.1"
                     /translation="MSDGANKVHKLQEKLSMKDFKLNQLLEITEAINSNSSVEKLLDI
                     YNYVLREQLGIQKAMLFNFDGDWKLLMKYGVKGSIKDIDVQKDLMKIKDITVIESSSK
                     KSLNTFDVAIPVF"
     gene            complement(345..1277)
                     /locus_tag="PK610_06995"
     CDS             complement(345..1277)
                     /locus_tag="PK610_06995"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRJ35716.1"
                     /translation="MRIDPSTTLKEIAALIGAKTVGDDNHSITGINEIHKVVPGDIVF
                     VDHPKYYDKALQSAATTIIINKDVDCPKGKALLISDDPFRDFNIITRHYSPSSTQHLE
                     VCHDSSIAETAFLYPNVVVGHNVTIGENTIIHPGVVIYDNAVIGNDVIIHANAVIGAH
                     AFYYKKRPSGFDKMHTCGRVVIHDKVEIGASTTIDSGVTGDTVIGEGSKIDNHVHIGH
                     DTVVGKKCLFAAQVGIAGCVVVGDNVTLWGQVGIPSGLKIGDGAVLLGQSGIMGDAEP
                     GKTYFGSPAGEHKTKYRELAALRQLPEILSKLKS"
     gene            complement(1288..2025)
                     /locus_tag="PK610_07000"
     CDS             complement(1288..2025)
                     /locus_tag="PK610_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35717.1"
                     /translation="MKIRAILFLAVLIPVYSFGQNIIIKQDVLADQEESNYGPNKKYY
                     KSTFTGLGLLFGKPDATGSAINGYKSFYYESGSRKKRQMGSFFAIGRDLSLNFRSFSI
                     KQNDSKTFGGIAQHKAERLFNINGNLLIYTRYNFKPKRGNQLGKYLDLAGYVEYAMIT
                     RHVVTDKLDEALGGNTIKSYYRGLPYLNKWNYGVTARLGFRHMLLFCQYRFSDMFNRS
                     PQFPYQELPRFCAGICFDVPDEFSRDR"
     gene            complement(2029..3723)
                     /locus_tag="PK610_07005"
     CDS             complement(2029..3723)
                     /locus_tag="PK610_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRJ35718.1"
                     /translation="MTRLLSSLLLLLLPFISVAQEKFWVFFTDKNGVDFDPYTFFDQK
                     AIERRLLHGIDLCDESDFPVNTDYVAAVGELCDSLSYSSRWFNAVAVYGNEGQMELIR
                     QLPFVQSVEAMDWYISNVTERRRIDLSDFNTGQVALVKGQVSRMQADKFTAAKIDGKG
                     IRICVIDAGFAGFTTCEALRHTYNRDYVKDTYDFTRRDKSVYHGHSHGTMVVSCIAGK
                     VDSIQLGLAPYAEFLLARTEKAFSDKLREEENWLAAVEWADKNGAQIINSSLGYTSSR
                     YFRSQMNGKAGLISRAATIAAHKGILVVSSAGNEGDGAWKIVASPGDADSILTVGGIN
                     PWTGLHATWSSYGPSTDHRLKPNVSAFGHAIVGHGQGYAEQVGTSFASPLVAGFAACV
                     FQMHPDWKNMKVLSEIESSSDLFPYYDYAHGYGVPQAGYFTDSVSTDTIPTFTIVEKE
                     GKFSVQIDSSHYSLAEPIQGTYHRGMPGESKYEMESDWKQHHIPDQELSSSSSYVYTQ
                     VPDYVFYHIENRDGYVKQYFVISVHQTEVLEFLRSDFTKGEKLRIWYKGFMKTIEF"
     gene            complement(3771..4841)
                     /locus_tag="PK610_07010"
     CDS             complement(3771..4841)
                     /locus_tag="PK610_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ35719.1"
                     /translation="MNTKKILLIEDNPEMRENTAEILELAKYNVVTAPNGKEGVKLAK
                     SESPDLIICDIMMPELDGYGVLHMLGKENETASIPFIFLTAKSEKSDLRKGMGMGADD
                     YLTKPFDDMELLTAVETRIQKSDRIKASFSKDLSGLNEFYQEARSLDDLKRLSENRKI
                     KSFRKKETIYSEGAFPNSIFFLASGKIKTALNNDDGKELIGGLYNQGEFFGYLALLEE
                     QPYADSAVALEDSEVVIIPKEDFFALLHSNREVAARFIRMLSDNIREREERLLKLAYN
                     SVRKRVAEALLLLEQRYSESRNEKGDFSMAISRDDLANIVGTASETVIRTLSDFKEEA
                     LIEIKGSRITLLNQDKLQRMKN"
     gene            complement(4847..>5836)
                     /locus_tag="PK610_07015"
     CDS             complement(4847..>5836)
                     /locus_tag="PK610_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ35720.1"
                     /translation="DLFAQTKDGREFPVEISLSHFSTSDGQFVMSFIIDITERKRQEA
                     ELKSASERLMQTSEALAKLNTDLENKVQERTKELEIANKDLNDSKKEVLKALEVEKEL
                     NNLKSRFVTTASHEFRTPLGTILSSASLIARYEGSEETEKRMKHVERIRSAVGNLTEI
                     LNDFLSVEKLEEGIVRNNPVQFDLEDFIHSIIEELKTITKKGQQIAFRHKGNTTSVFL
                     DNQLLRNSLINLISNAIKYSEEGKEITVQSTMNGNSIRIEITDQGIGIPPEDQQHMFG
                     RFFRAKNANNIQGTGLGLNIVRKYIELMEGKIGFSSELGVGTTFFVELPYLKP"
BASE COUNT         1582 a         1241 c         1212 g         1801 t
ORIGIN      
        1 aaatacagga attgcaacgt caaatgtatt cagggatttt ttagatgaag attcaattac
       61 cgtgatgtct ttgattttca tcagatcttt ctgtacatcg atgtctttga ttgatccctt
      121 cacaccgtat ttcatcagca gcttccagtc accatcaaaa ttgaacaaca tcgctttttg
      181 aataccgagc tgttcacgta atacgtaatt gtaaatatca agtagtttct caacagaact
      241 attggagttg atcgcttctg tgatctcaag caattggttc agcttgaaat ctttcatgct
      301 caacttctcc tggagcttat ggactttatt tgcgccgtcg ctcatcagga ttttagtttg
      361 cttaagatct cagggagctg acgcaatgca gcaagctctc tgtattttgt tttgtgttca
      421 ccggcaggac ttccaaaata agtttttccg ggttcagcat ctcccataat gccggattgc
      481 ccgagcaata ctgcgccgtc tccaattttc aagcctgatg gaataccaac ttgtccccac
      541 aaggtcacgt tgtcgccaac aactacacat cctgcaattc caacctgtgc ggcaaacaga
      601 catttttttc caacaacggt atcatgaccg atatgtacat ggttgtcgat tttgcttcct
      661 tctccaatca ccgtatctcc ggtaactccg gaatcgatgg ttgttgatgc gccgatctct
      721 actttatcat gaattactac tctgccacag gtgtgcattt tatcaaaacc acttggtctt
      781 tttttgtaat agaaagcgtg agcaccaatt actgcgttgg cgtgaatgat cacgtcattt
      841 ccaatcactg cgttatcgta aataacaacc ccgggatgaa taatggtgtt ttctccaatg
      901 gtaacattgt gaccaaccac aacatttgga taaagaaatg cagtctctgc aatagagctg
      961 tcgtgacaaa cttccagatg ctgagtgctt gacggagaat aatgacgggt aatgatattg
     1021 aaatcgcgga aggggtcatc ggaaatcaat aatgcttttc ctttcggaca atcaacatct
     1081 ttattgatga taattgtagt tgctgcagat tgcaacgctt tgtcgtagta tttaggatga
     1141 tcaacaaaaa caatatctcc gggtacaacc ttatgaattt cgttgatacc ggtaatggaa
     1201 tggttgtcat cgcctacggt ttttgcaccg atcaatgcgg caatttcttt cagagtggtg
     1261 gaagggtcga tcctcatgga aacaagatta acggtcgcgc gaaaattcgt caggaacatc
     1321 aaaacaaatg ccggcacaga aacgcggaag ttcctgatag gggaattgtg gactgcgatt
     1381 aaacatatcg gaaaaacggt actgacaaaa aagaagcata tgacggaatc ccaatcgtgc
     1441 agttacacca taattccatt tgttgagata gggtagtccg cggtaatagg atttaatagt
     1501 gtttccccct aaagcttcat caagcttgtc ggtgactaca tgtcgggtaa tcattgcata
     1561 ttcaacatac ccggccaaat cgaggtattt tccaagctga ttgccgcgtt tgggtttgaa
     1621 gttgtatctg gtatagatca gtaaattccc atttatatta aagagacgtt ctgctttatg
     1681 ttgtgcaata cctccaaaag ttttactgtc gttttgctta atactgaaac ttctgaaatt
     1741 gagactaaga tcacgtccaa tcgcaaagaa acttcccatt tgtctctttt tccgactgcc
     1801 ggattcataa tagaagcttt tgtaaccatt gattgcagaa cctgtagcat caggttttcc
     1861 aaaaagaagt ccgagtcctg taaatgtaga tttgtaatac tttttattcg gaccgtaatt
     1921 actttcttcc tggtcggcaa gtacgtcttg cttaataatg atgttttgtc cgaatgaata
     1981 aacaggaatc aaaactgcta agaataggat cgctcgtatt ttcataaatc agaattcgat
     2041 cgttttcata aaacctttgt accagattct caacttttct cctttggtga aatctgaacg
     2101 taaaaattca agtacttctg tctggtgaac cgatatcaca aaatattgct taacatatcc
     2161 gtcacgattt tcgatatggt agaatacata atccggtacc tgtgtataaa cgtaagagct
     2221 ggatgaagat agttcctgat ccgggatgtg atgttgtttc cagtcggatt ccatttcgta
     2281 ttttgattct ccgggcatac ctctgtgata tgtgccttga atgggttccg caagcgaata
     2341 atgtgaggag tcgatttgta ctgaaaattt cccttccttt tctacaatgg taaaagtcgg
     2401 gatggtatcg gtagaaacac tatcggtgaa gtatcctgcc tgaggaactc cgtatccgtg
     2461 tgcataatcg tagtatggaa aaagatcgga actggattca atctcagaaa gaactttcat
     2521 gtttttccag tcaggatgca tctgaaaaac gcaggcagcg aaacccgcca caagtggaga
     2581 agcgaaagat gttcccactt gttctgcata accttggcca tgtcctacaa ttgcgtgtcc
     2641 aaatgcggag acattaggtt tcaatcgatg atcggttgat ggaccatacg aactccaggt
     2701 agcatgtaaa cctgtccatg gatttattcc tccaacagtg agaatggaat cagcatcacc
     2761 cggtgaagca acaattttcc atgcaccgtc gccctcgttt cctgctgaac tgactaccaa
     2821 aatgccttta tgcgctgcaa ttgtagctgc tcttgaaatt aaaccggctt taccattcat
     2881 ctgcgagcga aaatagcgcg atgatgtata tcctaatgaa gaattaatga tctgtgctcc
     2941 gtttttatcc gcccattcaa cagctgcaag ccagttttct tcttctcgca atttgtcgga
     3001 gaatgctttc tctgttcttg ccagcaaaaa ttcagcatag ggagctaatc ccagttgtat
     3061 ggagtccact tttcctgcaa tacaacttac caccattgtg ccgtgtgagt gaccatgata
     3121 aacgctttta tctctgcggg taaaatcgta ggtgtctttt acgtaatcgc ggttgtaagt
     3181 gtgacgcaat gcctcgcagg tagtgaaacc ggcaaatcct gcatcgatga cacaaatgcg
     3241 gattcctttt ccatcaattt tggctgcggt aaacttatcg gcttgcattc gggaaacttg
     3301 tccttttacc aacgcaacct gaccggtatt gaaatcggaa agatcaattc ttctgcgttc
     3361 tgttacattg ctgatgtacc aatccatcgc ctctacactt tgtacaaagg gaagttggcg
     3421 gatcaattcc atttgacctt catttccata aactgcaaca gcattgaacc atcgtgaaga
     3481 gtagcttaag gaatcacata attcaccaac tgcagcaaca taatcggtgt ttaccggaaa
     3541 atcggattca tcacacagat cgatcccgtg cagaagccgg cgttcaatgg ccttttgatc
     3601 gaaaaaagta tagggatcaa aatcaactcc gtttttgtcg gtgaagaata cccagaattt
     3661 ttcctgtgca accgatataa acggcagcaa aaggaggagt agtgaagaaa gtagtctggt
     3721 catgggacgg gtgtccctac aaacatagca aaaaaatgac gcgtaacagc ttagtttttc
     3781 atccgttgca gtttatcctg gttgagcagg gtaatgcggc tacctttgat ctcgatcagt
     3841 gcttcttcct taaaatcaga gagggtgcga atgactgttt cagaggcagt tcctacgata
     3901 ttagcgaggt cgtcgcggga aattgccata gaaaaatcgc ctttttcgtt gcggctttcc
     3961 gaatagcgct gttcaagtag caataaggcc tcggccacac gcttgcgaac tgagttgtag
     4021 gcaagtttga gcagtcgttc ttccctttca cggatattgt ctgagagcat ccggataaaa
     4081 cgggcagcca cttcgcgatt ggagtgaagc agggcaaaaa agtcctcctt gggaatgatt
     4141 actacttcag aatcctccaa cgcaacagcc gagtcggcat agggttgttc ctcgagtaat
     4201 gccagataac cgaaaaattc accttgattg tacaatccac cgatcaattc tttcccgtcg
     4261 tcattgttga gtgcagtttt tattttgcca cttgcaagaa agaaaatgct gtttggaaaa
     4321 gcaccttcgc tgtaaatggt ttcttttttg cgaaaggatt tgatttttct gttttcggat
     4381 aatcgtttca gatcgtcaag actccgtgct tcctggtaga actcattcaa tccgctaaga
     4441 tccttggaaa aagatgcttt tatccggtct gatttctgaa tccgtgtttc tactgcagta
     4501 agcaattcca tgtcgtcaaa tggcttggtg aggtagtcat ctgcacccat ccccatgccc
     4561 ttgcgcaaat cagatttttc actttttgcg gtgaggaaaa taaaggggat agaggctgtt
     4621 tcgttttctt ttccgagcat atgcaataca ccgtagccgt ccaattcggg catcataatg
     4681 tcacaaatga tcagatcagg actttcgctt tttgcaagtt tcacaccttc ttttccattg
     4741 ggtgcagtaa ctacattgta ctttgccagt tctaaaattt cagcggtgtt ctcccgcatt
     4801 tccggattgt cttcaataag aaggattttt ttggtattca ttgttttcag ggtttcaaat
     4861 agggaagctc aacaaaaaat gttgtgccga caccaagctc actggaaaaa ccgatttttc
     4921 cttccattag ctcgatgtat tttcgtacaa tatttaatcc taatccggtg ccctgaatgt
     4981 tgttggcatt ctttgcacgg aagaaacgac caaacatgtg ttgctgatct tcaggaggta
     5041 taccaatgcc ttgatcggta atttcaattc gaatggaatt accattcatg gttgattgta
     5101 cagtgatttc ttttccttct tcagaatatt taatagcgtt gctgatgagg ttgatcaacg
     5161 aattacgaag taattgattg tcgagaaaaa cggaagttgt gttcccttta tgacgaaaag
     5221 cgatttgttg tccttttttc gtaatggttt taagttcttc aatgatggaa tgaatgaaat
     5281 cctctaaatc aaattgaaca gggttgtttc tgacaattcc ctcttccagt ttttctacgg
     5341 agaggaaatc gttaaggatc tcagtgagat taccaacggc cgaacgaata cgctccacat
     5401 gtttcatccg cttttcggtt tcttccgatc cttcgtaacg agcgatcagc gatgcagatg
     5461 atagaatagt tccaagtgga gttcgaaatt catgagaggc agttgtaacg aaccgcgatt
     5521 ttaaattatt aagttctttt tctacttcaa gtgcttttaa aacttccttt ttggagtcgt
     5581 tgaggtcctt attcgctatt tccagttcct tggttctttc ctgaactttg ttttccaaat
     5641 ccgtatttag ctttgccaaa gcctcggatg tttgcatgag cctttctgaa gctgacttaa
     5701 gttctgcctc ctgccgcttc cgttctgtaa tgtcaatgat aaagctcata acgaactgac
     5761 catcagaagt tgaaaagtga cttaagctga tttcaacagg gaattccctg ccatcttttg
     5821 tttgtgcgaa aagatc
//
LOCUS       DAOMYG010000177         5793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4723_length_5793_cov_10.282503, whole genome shotgun sequence.
ACCESSION   DAOMYG010000177 DAOMYG010000000
VERSION     DAOMYG010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4723_length_5793_cov_10.282503"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..637)
                     /locus_tag="PK610_07020"
     CDS             complement(32..637)
                     /locus_tag="PK610_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007840704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HRJ35721.1"
                     /translation="MILIDDKLISDELLQRRFVCDLSACKGACCVEGDAGAPLEKDET
                     EILEKIYPKIKPYLRKEGVKAIEEQGTWIEDSDKDLVTPLVNGAECAYVIFDDKGITK
                     CGIEKAWEEGKVKFRKPISCQLYPIRVKKTRTGEILNYDEWPICKPACACGDKLDVKV
                     YRFLKDAITRRYGKAFFDQLVEADKLLEKESEAGKTVKKKK"
     gene            888..2435
                     /locus_tag="PK610_07025"
     CDS             888..2435
                     /locus_tag="PK610_07025"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRJ35722.1"
                     /translation="MSVTNEDKLKLIISHCKEMGFVFPSSEIYDGLSATYDYGQLGAE
                     LKNNIRRYWWTSMVQMHENIVGIDAAIFMHPTTWKASGHVDAFNDPLIDNKDSKKRYR
                     ADHLLEDHMEKIRQKIAKEVEKAQKKFGGSFEEAMFLQTNPNVQRHVTKISEIEGRMK
                     QCLEDNDLEALRQLIVDLEIVCPISGTRNWTEVRQFNLMFSTELGSVAGDSATIYLRP
                     ETAQGIFVNFLNVQKTGRMKIPFGIAQTGKAFRNEIVARQFIFRMREFEQMEMQFFVR
                     PGTEMEWYNFWKETRMKWHMALGMGAEHYRFHDHIKLAHYANAACDIEYDFPMGFKEL
                     EGIHSRTDFDLGNHEKFSGKKLQYFDPELNQNYVPYVVETSIGLDRMFLAVLSHAYIE
                     EKLEDGSERVVLRIPPALAPVKAAVFPLLGKDGLPEKAREIFNLLKLDHNCQYEEKDA
                     IGKRYRRQDAIGTPYCITVDHQSLEDNTVTIRERDSMKQERVSIEQLPAIIDQRVNMK
                     YLLAKVK"
     gene            complement(2468..4747)
                     /locus_tag="PK610_07030"
     CDS             complement(2468..4747)
                     /locus_tag="PK610_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ35723.1"
                     /translation="MRNFYVCVLLFACMQFSINVSAQCTDLFISEYIEGSGNNKSIEI
                     YNPTSAPVNLSTYALRLYSNGSATPSANFTLAGTLNPGEVHIVSNPSANALILAISNQ
                     TSGSAINFNGNDVVELMNGATAIDRIGIIGNDPVTGWTVDGITNGTLNHTLRRKPGIQ
                     SGNTTWAGNAENEWTVYAVDVFKYLDFHVMNTCGSTLSATATMATFCLNEGFYFNSNA
                     TGGNSVYTYYWEFGDATLNVVNAQNGGYNYSVAGTYNVTLVVTDGNGHVFSLVQSVLG
                     HPAGPTACVTQTGGMNGCGTDTLQVSSCSTGGTGTLSYLWFFDNGNTSTATTPPIQYY
                     GPGTYNAYMVVTDANGCDDTTAGIFSVNATENPSFSYSQTLYCSSQSDPTPTIAGTNG
                     GTFSCNGCVINPITGEIDLSASGDGNFTIKYVTPGTCKDSSTVLVSITTTTANSTISP
                     AGPFCELSVPGNLTAATPGGTWSGNGIIDANLGTFDPSAAGVGTTIITYTIGGACGSS
                     DTETISVLANAEVQIVNSDTSICNDFFGFFLQANSGGVWSGTYVSDNNNGNGFFSAAA
                     IPAGNYYAVYSIAGTCGDKDSILVTVNPMPTALFSSAVVGLTIQFTDQSTGAISWDWV
                     ITEGANVTNFSTQNPVYSYIDPSVSVNACVNIVSSNGCEATYCQTILPTSTDELQLEK
                     INIYPNPAMGANISISGLHAASVITVYDLLGQKIQSIPFNNPNGSGQVNISEISGGTY
                     FIEIRSGNQSLTRKIVILH"
     gene            complement(4802..5092)
                     /gene="gatC"
                     /locus_tag="PK610_07035"
     CDS             complement(4802..5092)
                     /gene="gatC"
                     /locus_tag="PK610_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRJ35724.1"
                     /translation="MKIDEKTISRLAELSRLEFDPASAAEIQKDLTRIIGFIEKLNEL
                     DTEKVEPLIFMGDSENILREDVDVQTITQDEALKNAPRKDSDYFRIPKVLSK"
     gene            complement(5082..5702)
                     /locus_tag="PK610_07040"
     CDS             complement(5082..5702)
                     /locus_tag="PK610_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRJ35725.1"
                     /translation="MLQLLLFLPYKTIRPKGFGFPKGPFVVIANHTSYLDILIMHGIL
                     RGRQFIFLGKESLKKFPIIGMFFKSGNMHVPVKREDKSEASQSLRTMMDRVDQGYIAI
                     IFPEGTQSKQAPKMNPFKAGAFKVAIEKQIPILPMTILENFKLLSQVNRLTGPSRPGL
                     CKVIIHEPIVTEGMTIEDLLPLQERCKDLIESDLRKAYPKEYNNEN"
BASE COUNT         1632 a         1207 c         1251 g         1703 t
ORIGIN      
        1 ttaaccaaaa gaatcacttt tttatagtac cctatttctt tttcttaacc gttttacctg
       61 cttcagattc tttttcaagt agcttatccg cttcaaccaa ttggtcaaaa aaggctttgc
      121 cgtaacgacg tgtaatcgca tctttaagga aacgatagac tttcacatcg agtttatcgc
      181 cacatgcaca ggctggtttg caaatgggcc attcatcgta attgaggatt tctccggtgc
      241 gggttttttt aacacgaatg gggtagagct ggcaactgat gggcttacgg aactttactt
      301 ttccttcttc ccaggccttt tcaatgccac atttggtaat tcctttatca tcaaaaataa
      361 cgtacgcaca ctcagctcca ttaactaatg gtgtaacaag gtctttatcg ctgtcttcaa
      421 tccatgttcc ctgttcttca atggctttaa ccccttcctt gcgcaggtag ggttttattt
      481 tcggatagat tttctccaga atttcagttt catccttttc aaggggggct ccggcatcgc
      541 cttcaacaca gcaggctcct ttgcaggcag ataaatcgca tacaaacctg cgctgcagca
      601 attcatccga aataagttta tcatcaatca gtatcacctt acaaaagtaa caaaatagaa
      661 ccctttaacc agattcagtt accgacaggt acgaaaggat ttcacttaag taggcctgta
      721 tcaggttcac agaagttggg aagtctgatg aagtaggata gcgtgatgca tggttgaaag
      781 gctgttaatc gcttcaacag caatgatcaa ggctgatatt gcggagaaag gcaaaaattt
      841 caattcagac ttacggttct ccttcatctt cttacttttg cggcactatg agcgtaacca
      901 acgaagacaa actaaaactg atcatctcac attgtaagga gatgggcttt gtgtttccat
      961 ccagcgaaat ctacgatgga ctttcggcaa cctatgacta tggccagctg ggtgctgaac
     1021 tgaaaaacaa catccgccgg tattggtgga caagtatggt gcagatgcat gagaacattg
     1081 tcgggattga tgctgcaatt tttatgcatc caacaacatg gaaggcttcc ggacatgtgg
     1141 atgcatttaa tgatccgctg attgataata aggactctaa aaagcggtat agagccgacc
     1201 atttgctgga agatcatatg gaaaagatcc gccaaaaaat cgcaaaagag gtggagaagg
     1261 cacaaaagaa atttggtgga agttttgaag aagcgatgtt tctgcagacc aatccgaatg
     1321 ttcagcgcca cgtaaccaag atcagcgaaa ttgaggggcg catgaaacaa tgtcttgaag
     1381 acaatgatct cgaagcattg cgccaattga tcgttgacct cgaaattgtt tgtcccattt
     1441 caggaacgcg gaactggaca gaagttcgtc aattcaatct gatgttctcc actgagcttg
     1501 gttctgttgc tggagattca gctaccatat atcttcgacc tgaaactgcg caaggtattt
     1561 ttgtgaattt tttgaatgta cagaagaccg gtagaatgaa aatacctttc ggaattgcgc
     1621 aaacaggaaa agcattccgg aatgagattg ttgcccggca gtttattttc agaatgcgtg
     1681 agtttgaaca aatggaaatg caattttttg ttcgacccgg aacggagatg gagtggtata
     1741 acttctggaa agaaacacgt atgaaatggc atatggcttt aggaatggga gcagaacatt
     1801 atcgttttca cgatcacatt aagctggctc actacgcaaa tgccgcctgc gatattgaat
     1861 acgatttccc aatgggcttc aaagagctgg aaggaattca ttcgcgtacc gatttcgatc
     1921 tggggaatca cgaaaaattc tcaggcaaaa aattgcagta cttcgatccg gaattgaatc
     1981 agaattatgt tccttatgtg gtagaaacat ccattggatt ggataggatg ttcctggcag
     2041 tgctttctca tgcttacatt gaagagaaac tggaagatgg aagtgaacgt gttgtacttc
     2101 gtattcctcc tgcgcttgct cctgttaagg ctgctgtttt tcctctgtta ggaaaagacg
     2161 gtttacctga aaaggcaaga gaaattttta atttgctgaa actcgatcat aactgtcaat
     2221 acgaagagaa ggatgcaatt ggtaaacgct atcgccgtca ggatgcaatt ggaacaccct
     2281 attgcattac agtcgaccac caatcgctcg aagacaacac agtaaccatc cgcgaacggg
     2341 attcgatgaa gcaggaacgc gtttccattg aacagctccc ggcaataata gatcagcggg
     2401 tcaacatgaa atacctttta gctaaagtga aataaaaaaa tccccgattt tgtcggggat
     2461 tttttcttta atgcagtatc acgatttttc gtgtgagcga ttggttcccg gatcttatct
     2521 caataaaata agttcctccg gatatttcag aaatattgac ctgcccgctt ccattagggt
     2581 tgttgaaagg tatggattga attttctgtc ccaacaaatc ataaacagtg ataaccgaag
     2641 cggcatgcaa tccggaaata ctgatattcg ctcccattgc agggttggga taaatattga
     2701 tcttctccaa ttgcaattca tcagttgaag tgggtaaaat ggtttggcaa taagtggctt
     2761 cacatccatt tgaacttaca atgttcacac atgcatttac tgatacactc gggtcaatat
     2821 agctgtaaac cggattttgt gtgctgaaat tggtaacatt agcaccttca gtaattaccc
     2881 agtcccaact aatggcgccg gtagattgat ctgtaaattg aatggttaaa ccaacaacgg
     2941 ctgaactgaa aagtgctgtt ggcatggggt taaccgttac caaaattgaa tctttatctc
     3001 cacaggtacc ggctatggaa tacactgcat aataatttcc ggcaggaatt gccgccgcgg
     3061 agaaaaaacc gtttccgtta ttgttgtcgc taacataggt gcctgaccat actccgcctg
     3121 aattggcttg taaaaagaaa ccaaagaaat cgttgcaaat actagtgtcg gaatttacaa
     3181 tctgaacttc tgcattggca agtacagaaa tggtctccgt atccgatgat ccgcaagctc
     3241 ctcctatagt gtaggtaatg atggtagttc ctactccggc agcagatgga tcgaaggttc
     3301 cgagattggc atcaataatt ccgtttccac tccaggttcc tcccggtgtt gctgctgtaa
     3361 gattacccgg tacactgagt tcgcaaaacg gacctgcagg tgaaatggtg gaatttgcag
     3421 ttgttgttgt tatcgaaaca agtaccgttg agctgtcttt gcaagtcccc ggagttacat
     3481 atttaattgt aaagtttcca tcgcctgagg cggatagatc gatttctcct gttattggat
     3541 taattacaca tccattacaa ctgaatgttc ctccgttagt tcctgcaatt gttggtgtgg
     3601 gatcactttg tgaactgcaa tacaatgttt gactatagga gaatgacgga ttttcagttg
     3661 cattcacact aaatattcct gcagtagtat catcgcatcc atttgcatca gtaactacca
     3721 tataggcgtt gtatgtgccc ggaccataat attgaattgg aggtgttgtt gcagtactgg
     3781 tgtttccatt gtcaaaaaac catagatagc ttagtgtgcc tgttcctccg gttgaacaac
     3841 tgctaacttg taatgtgtca gttccgcaac cattcattcc tcctgtttga gttacgcaag
     3901 cagttggtcc tgcaggatga cccaaaacac tttgtacaag tgaaaagaca tgtccgtttc
     3961 catctgttac aacaagcgta acattatagg taccagcgac agaataattg taaccgccat
     4021 tttgggcatt aactacattt agtgttgcat ctccgaattc ccaataatac gtgtaaacac
     4081 tgtttcctcc tgtggcattt gaattgaaat agaaaccctc attcaggcaa aaagtagcca
     4141 ttgttgcagt tgcacttagt gtagaaccgc aagtgttcat tacatggaag tcgaggtatt
     4201 taaaaacatc aacagcgtat acagtccatt cgttttctgc gttgcctgcc caagttgtat
     4261 ttccgctttg aattcctggt tttctgcgca aggtatgatt aagcgttcca ttggtaatgc
     4321 catcaacagt ccatccggtt actggatcgt ttccaatgat tcctatacga tcaattgccg
     4381 ttgcaccatt catcagttca accacgtcat tgccattaaa gttgattgcc gatccgctgg
     4441 tttggttgga tattgcaaga attaatgcat tagcacttgg gttcgatacg atatgaacct
     4501 ctccgggatt gagtgttcct gctaaggtga aatttgctga aggtgttgca gaaccatttg
     4561 aatacaatcg gagcgcatag gtgctcaggt taactggggc agaagttgga ttataaatct
     4621 ctatggattt attattacca cttccttcta tgtattcgga aataaataaa tcggtgcatt
     4681 gtgcacttac attaatgctg aattgcatgc acgcaaacaa cagcacacaa acgtagaaat
     4741 ttctcataac aagttttttt ggtattgaaa attagggcaa aaagagatac cgaacaatac
     4801 tttatttgga aagtactttt gggatgcgga aataatctga atcttttcgc ggcgcgtttt
     4861 ttaaggcttc atcctgtgta atcgtctgca cgtccacatc ttcccttaga atattctcac
     4921 tatcacccat aaagataagc ggctcaacct tttcggtgtc cagttcattc agtttttcaa
     4981 tgaaaccgat tatccgggtg agatcctttt gaatttcagc tgctgatgcg gggtcgaact
     5041 ccagtcgtga caactcggca aggcgactta tggttttctc atcaattttc attgttgtat
     5101 tcttttggat aggctttgcg cagatcactt tcaattaaat ccttgcatcg ctcctgtaaa
     5161 ggtagtaaat cttcaattgt catcccctct gtcacaatcg gttcatgaat aatcaccttg
     5221 cacaatccgg gacggctggg tcctgttaac cggttgacct gagataagag cttgaaattt
     5281 tccagaatgg tcatcggaag aatcggaatt tgtttttcga ttgctacttt aaacgcgcct
     5341 gctttgaagg gattcatttt aggcgcttgt ttactttgcg tcccttccgg gaaaataatg
     5401 gcaatatacc cctgatcaac acggtccatc atggtgcgta aggattggga tgcttccgat
     5461 ttgtcttcac gttttaccgg gacatgcata ttcccgcttt taaagaacat gccgataatt
     5521 ggaaattttt ttagtgattc cttgcccaga aaaataaact gtcttcctcg aagtattcca
     5581 tgcatgatca gaatatcaag ataggaggtg tggttggcaa ttacaacgaa tggtcccttt
     5641 gggaacccga atcctttcgg ccgaatagtt ttatagggaa ggaacaacag aagctgcaac
     5701 atggggtacc agaaccaaca ttgtatggca aacaaaggac gccacgcccg caagggcagc
     5761 ggaaacggag ggcaaggcat gaacgccctc atc
//
LOCUS       DAOMYG010000178         5788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4733_length_5788_cov_8.873888, whole genome shotgun sequence.
ACCESSION   DAOMYG010000178 DAOMYG010000000
VERSION     DAOMYG010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4733_length_5788_cov_8.873888"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..4230
                     /gene="porU"
                     /locus_tag="PK610_07045"
     CDS             388..4230
                     /gene="porU"
                     /locus_tag="PK610_07045"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HRJ35726.1"
                     /translation="MVKSQELRYKIDLDWQNPARISPEEDINIKTMNFKGASFTGSEK
                     PQAFWAYSYDLPSSVNSITVRTENEVWEEVPTDQLVLLDESQIPEIFAVNAEVAYARF
                     QPRAVLSFFPYRKDLFSGKWMRLMEVDLLIGGTIGSPLQQRGLRTFAASSVLANDGEW
                     FRIGVIYDGVYKISYEFLKGLGVIAPAISSSEVHLFGNSAGMLAEANAISKPDDLLQN
                     AISMIDGGDGVFGPGDYFLFYAKGPHKVNYNASGQLFTQQVHLYCDTSYYFINVGSTS
                     ITQKLVSQQSSSSSPATHIVNTFDDFMYLEQDKINFIKSGREWYGDLYDFTTEYTYNF
                     AFPNIDLTQSVRVKTDLAARTPGSGASNFLVTSPGTTGSVTVVIPGVGTGAYTAAANP
                     GNGIMTFMPSSSNVSVKVKFNKATSTSQGWLNYIQVQARRNLIHFGDQMHFRDKNSVG
                     AGNVAEFVLSGVSGSMQLWDVTEPDNASQLNVSVVSGQIVFRTNADIMHEYIAFRSNS
                     CPTPFAFGKVSEQNLHSLSPRDLIIITHPQFYAQAAELAAFHESEGTGTILVTTDQVY
                     NEFSSGMKDATAIKQFLRMFYERAAGDPALLPKYVLLFGDGSYDNKNRVGGNTSFIPT
                     YQSFESTMVTVSFVSDDYFVHLDPTENMNNSDQLDMAIGRIPCRNQVEAQGVVNKIKL
                     YSQNTGTTSLESASACDLTGTEFSMGEWRNWYGFVADDEDFNSYIDAAEQFADSLKVW
                     YPFLNVDKVFLDAYLQQSTPGGQRYPEVNEAIRSRVEKGALVLNYIGHGGEVGWAHER
                     ILDLATVNGWTNNPRLPLFMTATCEFSRFDDPGRVSAGEYVLLNANGGGIGLLTTTRL
                     VYSGPNEILNRNFNKVVLVRDNGVPRTLGDIFMQTKNLTISQISTSNTRNFTLLGDPA
                     VRLKQPYNKIVADSVNHVAIGSGTDTLKALTRITISGHMEDQNGNFLSGYNGIVFPTV
                     FDKEQILYSLGNDPGSSARQFDLRKNVIYRGKASVVNGRFEFTFVVPKDIAYQFGIGK
                     ISFYAHNGTDDATGVDLSIIVGGTNPNAPVDNDGPIVRLFMNDENFVSGGTTDETPVL
                     LARVQDENGVNTVGTGIGHDITAILDGNTSSPVILNEYYESDANTYQSGVVTYPFRTL
                     SEGPHNLRFKVWDVYNNSTDASVDFVVARSAEMALKHVLNYPNPFTTRTQFFFEHNQC
                     CTMLDVQVQVFTVAGKLVKTLTKSVFAEGYRSDPIEWDGRDDYGDKIGIGTYIYRIRV
                     RTPDGKMVEKFEKLVILN"
     gene            4307..5599
                     /gene="porV"
                     /locus_tag="PK610_07050"
     CDS             4307..5599
                     /gene="porV"
                     /locus_tag="PK610_07050"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HRJ35727.1"
                     /translation="MIKPSYGFAALFAVSSLGVNAQSNQQARAEQIQLNTITTAVPFL
                     LIGPDSRSGAMGDAGVAISPDVNALHWNPAKLAFTKEQMEFSMSYSPWLRNLVPDINL
                     AYLSGYSRLDKYSVAGFGLRYFSLGNITFTDQNGSVIRDYKPNEFSVDGAYARKMSDR
                     FSLSMAGRFIYSNLTGGVIVQGANTKAGKSVAVDVSGYYINDDVKAGDMKAELAFGFN
                     ISNIGAKMNYTNTAERDFLPANLRLGTAFSLVPDEYNKVTFAFDVNKLLVPTRPIYRL
                     DNQGNILYDADGNPQILSGIDNNVGVAAGIFNSFHDAPGFFQTDQSGNPVYDANGDKV
                     IQKGSKFKEELREFNLSPGVEWWYANQFAVRAGYFHEHASKGNRKYFTLGAGVRYSVF
                     ALDVSYLVSISQQNPLNRTLRFTLRFTFASKKDKDKVD"
     gene            5599..>5788
                     /locus_tag="PK610_07055"
     CDS             5599..>5788
                     /locus_tag="PK610_07055"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRJ35728.1"
                     /translation="MKLRIGYGFDVHRLSEGYDLWLGGVKIPAEKGAVGHSDADVLLH
                     AICDAMLGALSLRDIGHHF"
BASE COUNT         1568 a         1115 c         1341 g         1764 t
ORIGIN      
        1 cttggatccc gcgaaggctg ggttcaaata tagcgggttg gcgaagaatt gcgtgaattc
       61 agggtcttgt gccttggcat tttcaggcaa aagcatgaca atgctgaaca gcataaggaa
      121 cagataatga gcgggtctgg tgtaaaggtt gaacctcata ggaaacctga tttctgaatt
      181 ttaaggacca atattactaa tattttttaa catgaattgt tcatatgtca ataacaatga
      241 aaatatcttg gggatttgta cgttattttg gggaacaaaa gttgcccttt acttgttagt
      301 gtgctgataa tgagtcaaat taatttttat atgccttatt cccggaagac aactctcctg
      361 ctgattcttt tcattgggtt ctttcaagtg gttaaatcac aagagcttag gtataagatt
      421 gacctggatt ggcaaaatcc tgcccggatt tctcctgaag aagacatcaa tattaaaaca
      481 atgaatttca aaggcgcttc ttttacagga agcgagaaac ctcaggcttt ttgggcttat
      541 tcctatgatt tgccttcatc tgttaacagt attactgtaa gaacggagaa tgaagtttgg
      601 gaagaagtac caactgatca gctcgtattg ctcgatgaat cccagattcc ggagattttt
      661 gctgttaacg cggaagtagc ttatgcccgg tttcagccaa gagctgtatt atcgtttttc
      721 ccttatagaa aagatctttt ttcaggtaaa tggatgcgtt taatggaagt cgatctgctt
      781 attggaggaa caatcggatc tccattgcaa cagagaggtc tgcgaacatt tgcagcttct
      841 tctgttttgg caaacgatgg agaatggttc aggattggtg tcatttatga tggagtatat
      901 aaaatatcct atgaattttt aaaaggtttg ggtgtaatcg cccctgctat ttcatcttca
      961 gaagttcatc tctttggaaa ttctgcagga atgcttgccg aagccaatgc catttccaag
     1021 cccgatgatt tgctgcaaaa tgcaattagt atgatcgatg gtggagatgg tgtttttggt
     1081 ccgggtgatt attttttatt ctatgcgaaa ggtccgcaca aagtgaatta taatgcttca
     1141 ggacaattgt tcactcaaca ggtgcatttg tattgcgata cttcctatta ttttatcaat
     1201 gttggttcaa cttctattac acagaaatta gtcagccaac agtcttcttc atcttctccg
     1261 gcaacacata ttgtcaatac gtttgatgac ttcatgtatc tggagcagga taagatcaat
     1321 tttattaagt cgggcagaga atggtatgga gacttatacg atttcactac agaatacacg
     1381 tataattttg catttccgaa catcgattta acgcagtctg tacgcgtgaa gacagatctt
     1441 gctgcacgaa ctcccggaag tggtgctagt aattttcttg ttacatcacc aggtacaaca
     1501 ggaagtgtta ctgttgtcat tcccggagtt ggaaccggtg cttatactgc ggctgcaaat
     1561 ccaggaaatg gaattatgac ctttatgcca tcatccagta atgtctctgt aaaggtaaaa
     1621 ttcaacaagg ccacatctac atcgcagggt tggttgaatt atattcaggt tcaggcgcgg
     1681 cgcaatctta ttcattttgg cgatcaaatg catttccgcg ataaaaattc ggtgggagca
     1741 ggcaatgttg ctgagtttgt tttgagtggc gtttccggtt ctatgcagtt atgggatgtt
     1801 actgaacccg acaatgcatc acaacttaat gtatcggttg tttccggaca aattgtgttc
     1861 agaacaaatg cagatatcat gcacgagtat attgcattta ggagcaatag ttgtccaacc
     1921 ccatttgcgt ttggaaaagt aagtgaacaa aatttacatt cactctcacc acgtgatctt
     1981 atcatcatca cacatcccca gttttatgca caggctgcag agcttgcagc tttccatgaa
     2041 tcagaaggaa caggaacaat tctcgtaact accgatcagg tttataacga gttttcttca
     2101 gggatgaagg atgcgacagc catcaagcaa tttcttcgta tgttttatga gcgcgctgca
     2161 ggtgatcctg cattgttgcc gaaatatgtt ttgctttttg gagacggatc ttacgataat
     2221 aaaaacagag taggagggaa tacctcgttt attccgacct atcaatcgtt cgaatccaca
     2281 atggtgacgg tgagttttgt gagcgacgat tatttcgttc atcttgatcc gactgaaaac
     2341 atgaataatt ctgatcagct ggatatggcg attggaagaa taccgtgccg taaccaggtg
     2401 gaggcacaag gtgtggtcaa caagattaaa ctttattcac agaataccgg aacaacttca
     2461 cttgaatcag cctctgcatg tgatttgaca ggaacagaat tttcgatggg tgaatggaga
     2521 aactggtatg gttttgttgc cgatgatgaa gatttcaatt cgtacatcga tgcagcagaa
     2581 cagttcgcag attcacttaa agtgtggtat cccttcttga atgtcgacaa agtttttctt
     2641 gatgcctatt tgcagcaaag tactcccggc ggacaacgtt acccggaggt gaatgaagcc
     2701 atccgatcga gagtggaaaa aggtgctttg gtattgaatt atattggtca cggtggtgaa
     2761 gttggttggg cgcatgaacg tattttggat ctggctacag taaatggatg gaccaataac
     2821 ccacggcttc cgttattcat gacagccact tgtgagtttt cgcgtttcga tgatcccggt
     2881 cgtgtatctg caggtgagta tgttttactc aatgccaatg gaggaggaat tggtttattg
     2941 accaccacac gattggtgta ttcaggtcct aacgagatcc tgaaccgaaa tttcaataaa
     3001 gttgtgttgg tacgtgataa tggcgttccg cgtacgctgg gtgatatttt tatgcagaca
     3061 aaaaatctta ccatcagtca gatttcaact tccaataccc gcaatttcac attgcttgga
     3121 gatcctgctg tccgactgaa gcagccgtac aataagattg ttgcggattc tgtaaatcac
     3181 gttgctattg gatcaggaac agatacatta aaagctctta ccagaattac catttccggt
     3241 catatggaag atcaaaacgg taacttttta tccggataca acggcattgt ctttccgact
     3301 gtctttgata aggagcagat tttgtattca cttggaaatg atcccggttc ttctgctcgt
     3361 cagttcgact tgcgcaagaa tgtgatatac cgtggaaaag cgtcggttgt taatgggcgc
     3421 ttcgaattca cgtttgtagt tcccaaagac atcgcttatc agttcgggat tggaaaaatt
     3481 tccttctatg ctcacaatgg tacggacgat gcaacgggcg ttgatttgag cattattgtt
     3541 ggaggtacca atccaaatgc accggttgat aatgatggtc cgatcgttcg gcttttcatg
     3601 aacgatgaaa actttgtatc aggaggaaca accgatgaaa ctccagttct tttagcccgg
     3661 gttcaggatg agaatggagt gaacacagtt ggaaccggga ttggtcacga cataactgcg
     3721 atattggatg gcaatacctc ttctcctgta attctcaatg agtattatga atcggatgcc
     3781 aatacttatc agtcgggagt agtaacctat ccgttccgca ccctgagcga aggccctcac
     3841 aatctccggt tcaaagtttg ggatgtgtac aacaattcaa cggatgcaag tgtggatttt
     3901 gttgttgcgc ggtctgcaga aatggctttg aagcatgtcc tgaactaccc gaatcctttt
     3961 accacccgaa cccagttctt ttttgagcac aaccagtgtt gtaccatgtt ggatgtacag
     4021 gttcaggtat ttaccgttgc cggaaagctg gttaaaacac ttacaaagtc ggtttttgcc
     4081 gaaggttacc gatccgatcc aatcgaatgg gatggccggg atgattatgg tgataaaatc
     4141 ggtattggca cctacatcta tcggatacgg gtgcgcactc cggatggtaa aatggtggag
     4201 aaattcgaaa aactggtcat tttgaactaa ttcagtcaaa cggggttcta caataaaagc
     4261 acgctttttc cgtatatttg ttgactttta tttatccaat acgatcatga tcaaaccatc
     4321 ttacggattt gcagccttgt ttgcggtttc ttctttgggg gtcaatgccc aaagcaatca
     4381 gcaggcgcgt gcagagcaaa ttcagctcaa tacaattact acagcagtac cttttctgtt
     4441 aatcggtccc gactcccgtt ccggcgcaat gggggatgca ggagtagcca tttctccgga
     4501 tgtaaatgca ttgcactgga atccagcaaa actagccttt accaaggagc agatggagtt
     4561 ttcaatgagc tattcacctt ggttacgaaa tcttgttccc gatatcaacc tggcctattt
     4621 gtcggggtat tcccgtctcg ataaatattc tgtggctgga tttggacttc gttatttttc
     4681 gcttggtaac atcacattta ccgatcagaa cggaagtgtg attcgtgatt acaaacccaa
     4741 tgaattttcg gttgatggtg cttatgcacg taagatgtcg gatcgttttt cattatcgat
     4801 ggccggacga ttcatttact caaatctaac cggtggtgta attgtgcagg gtgccaatac
     4861 caaagccggc aaatcggtgg cggtagatgt ttccggatat tacatcaatg atgatgtgaa
     4921 ggcaggtgat atgaaagcgg aacttgcgtt cggatttaac atcagcaata ttggagcaaa
     4981 aatgaattat accaatactg cagagcgcga ctttcttcct gcaaatcttc gtttgggaac
     5041 agctttttcg ttggtacctg atgaatacaa caaggttact tttgcatttg atgtgaataa
     5101 attattggtg cctacgcgtc cgatttaccg tttggataat cagggtaata ttttatatga
     5161 tgcagatgga aatccgcaga tcttaagtgg aattgataac aatgtgggag ttgctgccgg
     5221 aatattcaat tcgtttcatg atgctccggg tttttttcaa actgatcaaa gcggaaatcc
     5281 ggtttatgat gccaacggcg ataaggtcat tcaaaaagga agcaagttta aggaagagct
     5341 gcgcgagttc aatttatctc ccggagtaga gtggtggtat gccaatcagt tcgctgttcg
     5401 tgccggttat ttccatgaac atgccagcaa aggaaaccgt aaatacttta cactgggtgc
     5461 aggtgtgcgt tatagcgtat ttgcacttga tgtgagttat cttgtttcga tctctcagca
     5521 aaatcctttg aacagaacct tgcgctttac cttgcgcttt acgttcgctt caaaaaagga
     5581 taaggataaa gtcgattaat gaaacttcgg attggttacg gattcgatgt tcatcgcctg
     5641 agtgaaggtt atgatctctg gctgggtggc gtaaaaattc ctgcagaaaa aggagccgtt
     5701 ggtcattctg atgctgatgt gctattacat gccatttgcg atgccatgct gggcgcttta
     5761 tccttgcgtg atatcggaca tcattttc
//
LOCUS       DAOMYG010000179         5776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4749_length_5776_cov_8.520888, whole genome shotgun sequence.
ACCESSION   DAOMYG010000179 DAOMYG010000000
VERSION     DAOMYG010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4749_length_5776_cov_8.520888"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PK610_07060"
     CDS             complement(<1..198)
                     /locus_tag="PK610_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35729.1"
                     /translation="MKKVFLLLFLVASIGASAQFKLDEADYGTKFKTLFDAAKKRFAT
                     EKTGAKTTISEGDFVAKYETTI"
     gene            complement(237..1715)
                     /locus_tag="PK610_07065"
     CDS             complement(237..1715)
                     /locus_tag="PK610_07065"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HRJ35730.1"
                     /translation="MKSRFLLLAMFAFPVFAIAQQEYSSFTSTGRGGATTFVTDYQAL
                     GINPAGVGMANPYGKKMTMGFTEFTFSLHSQALTKQELRSTIGSYVKGENQQTFTQQQ
                     KIDAAREFTDAGFAVNVDFGSFGFSFCDPKIGGFAFRVNDRFQWYSKLGKEASELLFL
                     GFNANYFDSLVYNNGMGNTVIANNPNMSQDSINNVVLGFTNTPKMISQLLDGTVMNMS
                     WTREYNLSYGRKVFGNDSTFALYGGVGVKLYQGLAYMDVNASNGQLYAFSSISPAFNI
                     DYGTAAQSNPSSVTQTGKIPKPVGMGYGFDFGVNLLIKGKLKIGAAMSNMGSITWDGN
                     VYTMKDTLVFDTDNPGAENYNIIGQFNDMGGDSGLFKWDGVSEKKVSLPTTYRFGASM
                     KLGKVAELGFDVIIPGNDEAGNFRKPIIGMGGDITPIRWVRFSAGFMTGGNYDFMIPL
                     GVTFIAQDGRWEGGIASRDVVTFFTQNGPTISFSTGFMRFRF"
     gene            complement(2111..2992)
                     /gene="hemF"
                     /locus_tag="PK610_07070"
     CDS             complement(2111..2992)
                     /gene="hemF"
                     /locus_tag="PK610_07070"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HRJ35731.1"
                     /translation="MTREHIESTFRTIQDEICSGLEQLDGKAFFQEDNWQRDGGGGGR
                     TRVISHGTLLEKGGVNFSAVHGELPAALKKEFGADNVQFFATGVSIVLHPVNPWVPII
                     HMNIRYFEMSSGESWFGGGIDLTPHYVRPQQADLFHQQMKNVCDRFNTEYYPKFSKWA
                     DDYFLIRHRNETRGIGGIFFDHLKPESESHKQSIFDFVCAVGRTFVPAYAEQVALNRN
                     LPYGEREVKWQHLRRGRYVEFNLVYDRGTKFGLETNGRIESILMSLPETASWLYNYQP
                     EPGSEEEKTLKYLQKVR"
     gene            complement(2989..5061)
                     /locus_tag="PK610_07075"
     CDS             complement(2989..5061)
                     /locus_tag="PK610_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRJ35732.1"
                     /translation="MIKRHLILFLYCIILCSVPLVSMAQPGDAKKETIEGKEYYIHTV
                     AKGETVYGISKIYKVSQEQILQSNPEAAKGLDVGQKLKIPVASSAVKDKTGADATEKE
                     YKALMAEGERFMSIQDYPGAEDAFIRALKVKPNDAIATSRIKEARDLAKAKKEVSPEP
                     AKNIEEGFTSYTVEKKETLYGISKKTGVAQEKILELNPDAKDGLKEGQVLRLPSAKKQ
                     NEPVGTIKNETPKDIPLGNTVLIEHVVAKGESIYGLSRKYKVSEDSLKIYNNGLQEGL
                     KLGQTLVVPVSKKYAEENNLRSYPDLRTMQADQKQNRERISNSTFEVALMLPFCLDKN
                     QSLMESRSSSANSPVELFEPTRQSLDFYHGVMLAVDSLRQYGLNIHLHVFDTARDSAK
                     LSKYVKEAEFAGLDLIIGPTEQVEMVAKAASEKKIAMICPFAYTNKILLGNATVSKAV
                     TTASVMINAVADHIGKLYATENIFLIDTRSKKDQPLYNAFRSELNAKVLSNGAKDSVK
                     MIRTEASSSKAWIDRLSKEKVNIFVIPSTDLSFVNSFFNTLQGVSLKTQYKDYQFMIF
                     GLEEWLKYDEIEANLKVKFNLHLPSPVFPSFVDSARTIPFIKAFRSKFSTDPDRFAMM
                     GFDVAYFYLGGFLHEGRDFSSHLDRYDVELVHTRFRFRKMGEDSGFLNDHSYILRYQD
                     FQLKVVYP"
     gene            complement(5058..>5776)
                     /gene="guaA"
                     /locus_tag="PK610_07080"
     CDS             complement(5058..>5776)
                     /gene="guaA"
                     /locus_tag="PK610_07080"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015782888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRJ35733.1"
                     /translation="GVDAKKRFYDALAGITDPEQKRKAIGRVFIEVFDDESKKHATAK
                     WLGQGTIYPDVIESVSVKGPSATIKSHHNVGGLPDFMKLSVVEPLRTLFKDEVRKVGK
                     ALEIDQAILGRHPFPGPGLGIRILGDITAEKVAILQEVDAIFINGLKKHNLYNEVWQA
                     GAILLPVQSVGVMGDERTYENAVALRAVSSTDGMTADWCHLPYEFLAHISNEIINKVK
                     GINRVVYDISSKPPATIEWE"
BASE COUNT         1589 a         1369 c         1162 g         1656 t
ORIGIN      
        1 aatggtagtt tcatatttgg ccacaaaatc accttcgctg attgtggttt tggctccggt
       61 tttttccgtt gcaaaccgtt tttttgctgc atcaaacaag gtcttgaact tagtgccata
      121 atcggcctca tcaagcttga attgcgctga agcaccaatt gatgccacta aaaagagaag
      181 tagaaagact tttttcatgt gcttattttt caaaatcaac tgcaataatc agaccatcag
      241 aatcggaatc gcataaaacc ggtgctgaat gaaatggttg gtccgttctg cgtaaagaag
      301 gtcaccacat cacgggaagc aattccacct tcccagcgac catcctgagc gatgaatgtg
      361 actcccagag ggatcataaa atcataatta ccgcctgtca tgaatcctgc tgaaaaccga
      421 acccagcgaa tcggggtgat atctcctccc ataccaatga tcggcttgcg gaagttccct
      481 gcctcatcat ttccgggaat gatcacatca aatcccaatt ctgctacttt acccagtttc
      541 atactcgctc caaaacggta agtggtgggc agactcacct tcttttcaga aacaccatcc
      601 cacttaaaca atccgctgtc gccacccata tcgttgaact gaccaataat attataattc
      661 tccgcacccg gattatccgt atcaaaaacc aaggtatcct tcatggtgta cacatttcca
      721 tcccaggtaa tgcttcccat attcgacatc gctgcgccga ttttcaactt acctttaata
      781 agcaagttca cgccaaagtc aaatccgtat cccattccta ccggcttagg tatctttccg
      841 gtttgggtaa cagatgaagg attgctctgc gctgctgttc cgtaatcgat gttgaaggcc
      901 ggagatatcg atgaaaaagc atacaactga ccattcgatg cgttgacatc catataggcc
      961 aatccctgat acagcttcac ccctacacca ccatacaaag caaaggtact gtcgttacca
     1021 aaaacctttc ttccatagga aagattgtat tcccgggtcc aggacatatt catcactgtg
     1081 ccatcgagca actgagaaat catttttgga gtattggtaa aaccaagaac cacattattg
     1141 atactgtcct gcgacatatt cggattgttg gcgattaccg tatttcccat tccgttattg
     1201 taaaccagac tatcgaaata atttgcatta aacccgagga agagcaattc gctggcttct
     1261 tttccgagtt tggaatacca ctgaaaacgg tcgttaactc gaaatgcaaa cccgccaatt
     1321 ttcgggtcgc agaatgaaaa accaaacgat ccgaaatcca cgtttaccgc aaatcctgca
     1381 tctgtgaatt cgcgtgcagc atcaattttc tgttgttgcg tgaatgtttg ctgattttca
     1441 cctttcacat acgaaccaat ggtactgcgc aattcctgtt tggtgagcgc ctgtgaatga
     1501 agtgaaaaag taaactccgt aaatcccatt gtcatttttt tgccatatgg atttgccata
     1561 ccaacaccgg caggattaat tcccaaggcc tgataatcgg taacaaaagt tgttgcccca
     1621 ccgcgaccgg tagatgtaaa agaagaatat tcctgctgtg caattgcaaa aacaggaaaa
     1681 gcgaacatcg ccaaaagaag aaatcgagat ttcataccgc agaaatttgt gcgaaaagat
     1741 agcttttttt tgtttcagat gaataagcca attgaccaat tacagattac taattaccag
     1801 ttatctgata acccagttac ctaattaccc aacttgtccg ccgaaggttt accgaaggag
     1861 gattgaccaa ttacccaact tgtccgccgt agtgaaacga aggaggatta cccagttacc
     1921 caacttgtcc gccgaaggtt taccgaagga agattaccca atcaaccaat tacccaactt
     1981 gtccgccgaa ggtttaccga aggaggatta ccaaattaac caattaccta gttacccaat
     2041 taccaatgac aaataacttc ttcccattac atcatgggtc tgaattctat cgccttagca
     2101 aacaattgaa ttatctgact ttttgaaggt acttcagggt tttttcttct tctgaaccgg
     2161 gttccggttg atagttgtac agccaggatg ctgtttctgg caggctcatg agtattgatt
     2221 cgattctgcc gttggtttcg agtccgaatt tggttcccct atcgtaaaca aggttgaatt
     2281 cgacataacg accgcgacgc aaatgctgcc attttacctc tcgttcgccg taaggaagat
     2341 tgcggtttag tgcgacctgt tcggcatagg caggtacaaa agttcgtcct accgcgcaga
     2401 caaaatcaaa aatcgattgc ttatgtgatt cactctcagg tttaagatga tcgaagaaaa
     2461 taccgccaat tccgcgggtt tcattgcggt gacggatcag aaaataatca tcagcccatt
     2521 tactgaattt gggataatac tcggtgttga agcgatcaca cacatttttc atttgctgat
     2581 gaaacagatc agcttgttgc ggtcgaacat aatgcggtgt gagatcaata ccaccaccaa
     2641 accaggattc tccactgctc atttcgaagt agcggatatt catgtgaatg atagggaccc
     2701 agggattaac aggatgcaaa acaatggaaa ctcctgtagc gaaaaactga acattatctg
     2761 caccgaactc tttctttagt gcagcaggta attcgccatg aacagcagaa aaattcacgc
     2821 cgcccttttc gagcaatgtg ccatgagaaa taaccctcgt gcgcccaccg ccaccgccat
     2881 cgcgttgcca attgtcttcc tgaaaaaacg ctttcccatc cagctgttcc agtccgctgc
     2941 aaatttcgtc ctgaatagtt ctgaaggtgc tttcgatgtg ctcgcgtgtc atggatacac
     3001 tactttcagt tgaaaatcct gataacgtag aatatacgaa tgatcgttca ggaacccgct
     3061 gtcctctccc attttacgga agcgaaagcg cgtgtgaacc aattcaacat cgtacctgtc
     3121 gaggtgtgaa gaaaaatccc ttccctcatg cagaaagccg cccagataaa aataggcgac
     3181 atcgaatccc atcatcgcaa aacgatcggg gtcggtactg aacttggagc gaaatgcttt
     3241 gataaaagga attgtccggg cagaatccac aaaagatgga aacaccggcg atggcaaatg
     3301 aaggttgaat ttcactttca gattggcttc gatctcatca tatttcaacc attcttccaa
     3361 tccgaaaatc ataaactgat aatccttgta ttgtgttttg agcgatacac cctgaagggt
     3421 attaaagaag ctgtttacaa aactcagatc agtacttggt atgacaaaaa tattgacttt
     3481 ctccttactc aaacgatcta tccatgcctt ggaagatgat gcttcggtgc ggatcatttt
     3541 tacggaatct tttgctccgt tactcaaaac ttttgcattg agttccgaac gaaatgcgtt
     3601 gtacagtggc tgatcttttt tcgaacgggt atcaatcagg aaaatatttt ctgtagcgta
     3661 caactttccg atatgatcgg caacagcatt gatcatcacc gatgcagtgg ttaccgcttt
     3721 ggatacagtg gcattaccaa gtagaatttt attggtatag gcaaacggac aaatcatggc
     3781 aatctttttt tctgatgccg cctttgcaac catttcaacc tgttcggttg gaccaataat
     3841 cagatccaat cctgcgaact ctgcctcttt tacatatttt gaaagttttg cagaatcgcg
     3901 cgccgtatca aaaacatgca aatgaatatt taaaccatac tgacgtagtg aatcaactgc
     3961 aagcatcaca ccgtggtaaa aatcgagcga ttgccgtgtg ggttcgaata attcaacagg
     4021 cgaattggcg gaagaggaac ggctctccat taaggattga tttttatcca gacaaaaggg
     4081 aagcatcaat gcaacctcaa atgtagaatt ggaaatccgt tcacggtttt gtttttgatc
     4141 ggcctgcatt gtgcgcagat caggataaga acgcaaattg ttctcttcgg catatttttt
     4201 cgagaccgga acaacaaggg tttgccctaa tttcaatcct tcctgcaatc cgttgttata
     4261 gattttcagc gagtcttccg atactttgta tttacgcgat aaaccataaa tggattctcc
     4321 tttggcaacc acatgttcga tcagaacggt attgccgagc ggaatatctt ttggtgtttc
     4381 atttttaata gtgccaacag gctcgttttg ctttttagcg gaaggcaaac gaagaacctg
     4441 accttctttt agtccgtcct ttgcatcggg attcagttca agaattttct cctgcgcaac
     4501 tcccgtcttt ttggaaattc cgtaaagggt ttcttttttc tcaaccgtat aagaggtgaa
     4561 tccttcttca atgtttttag caggttcggg agagacctct ttcttcgctt ttgccaggtc
     4621 tcttgcttct ttgatacgcg aagtggcaat tgcatcattc ggcttcactt tcaaggcacg
     4681 gataaacgca tcttcggcac ccggataatc ctgaatgctc ataaaccgct cgccctcggc
     4741 cattagtgcc ttgtattctt tttcggtggc atcggcacca gttttgtctt tcaccgctga
     4801 agaagcaacg ggaatcttta gtttttgtcc gacatccaat cctttggcag cttcggggtt
     4861 tgattgcagg atttgttcct gactaacctt ataaattttc gagataccgt aaactgtttc
     4921 tccttttgca acagtgtgga tgtagtattc ctttccttcg atcgtttctt ttttggcatc
     4981 gccgggttga gccattgaaa caagaggtac actgcataga ataatgcaat acagaaaaag
     5041 tatcaggtgc cgttttatca ttcccattcg attgttgcag gtggttttga gcttatatcg
     5101 tacacaacgc gattgatgcc tttcacctta ttgatgatct cattcgaaat gtgtgccaga
     5161 aattcgtaag gaaggtgaca ccagtcggcc gtcatgccat ctgtactgct tactgccctg
     5221 agtgccacgg cattttcata ggtacgttca tcgcccatca ccccaacact ttgcacagga
     5281 agcaggatcg ctccggcttg ccatacttca ttgtagaggt tgtgtttctt cagtccgttg
     5341 ataaaaatgg catcgacttc ctgcaggatg gcgactttct cagctgtaat atccccaaga
     5401 attctaattc ccaatccggg tcccggaaag gggtgtcggc ccaaaattgc ctgatcgatc
     5461 tccagcgctt ttcctacctt gcgtacttcg tccttaaaca aggttcgcag aggctcaaca
     5521 acactgagct tcataaaatc aggtaagccg ccaacattgt ggtgcgattt aatggttgca
     5581 ctcggacctt ttaccgatac cgattcaata acatcagggt aaatggtgcc ctgacccagc
     5641 cacttcgcag ttgcatgttt tttggactca tcatcaaaaa cctcaataaa tactcttcca
     5701 atcgctttac gtttttgctc aggatcagta attcctgcaa gcgcatcgta gaatcgtttt
     5761 ttagcatcca caccgg
//
LOCUS       DAOMYG010000180         5758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4766_length_5758_cov_8.542697, whole genome shotgun sequence.
ACCESSION   DAOMYG010000180 DAOMYG010000000
VERSION     DAOMYG010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4766_length_5758_cov_8.542697"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="PK610_07085"
     CDS             complement(<1..1264)
                     /locus_tag="PK610_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HRJ35734.1"
                     /translation="MIKSLHILITFLLFGGSISYAQNTGTKGKNPPPANVELLNADKV
                     IFDQRKGDAQRALGNVQVRHDNTLMYCDSAWIYQKRNVLFAFGHLHVKQGDSLNMYGD
                     SLFYDGNTGYGKLRGHIRMVELDMSMETDSLEFVSKEGRGWYSTGGIIRSAKNKNVLT
                     SKFGEYRSASKDIFFSGEVMLKHPDYTMTCDTLRYHTPTETAWFYGPTVLRTNDGVIL
                     CEGGWYNTRKDQSRFIKNVYMSNGEYKFRGDTLDYSQKSGLGTARGFFSLYDTTNKST
                     LSGKYGWFNEKLKSAFVTDSALMMRPFDDDTLYLHADTLFASREGKDSTQWMRAWRNV
                     RFFRNDVQGMCDTLDYHEKDSVLNLLGKPVIWQDDQQLSAQRMEVHFRNKELHRIDLI
                     GNCFISSFADSLCYNQIKGRTMHGYFKDE"
     gene            complement(1357..2583)
                     /locus_tag="PK610_07090"
     CDS             complement(1357..2583)
                     /locus_tag="PK610_07090"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014158803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRJ35735.1"
                     /translation="MSKIKVANPVVELDGDEMTRIIWKFIKDKLILPYLDVDIKYYDL
                     GMENRDATNDQVTIDAANAIKQHGVGIKCATITPDEERVKEFNLKQMWKSPNGTIRNI
                     LDGTVFREPIVCKNVPRLVTTWDSPIIVGRHAFGDQYRATDFVVPGKGKLMVKFTGED
                     GTVIEQEVYSFNGPGVALSMYNVDESIRGFASACMNMALVKGWPLYLSTKNTILKKYD
                     GRFKDIFQEVYDAEFKDKFKAAGITYEHRLIDDMVASAMKWSGKFVWACKNYDGDVQS
                     DSVAQGFGSLGLMTSVLVTPDGKVMEAEAAHGTVTRHFREHQKGNRTSTNPIASIFAW
                     SRGLAFRGKLDNNQELINFADALEKVCVETVESGKMTKDLAVCIYGDKVTADNYLYTE
                     DFLNEIDKGLKAKLGK"
     gene            complement(2733..4433)
                     /locus_tag="PK610_07095"
     CDS             complement(2733..4433)
                     /locus_tag="PK610_07095"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HRJ35736.1"
                     /translation="MIGFFFRQLRHARRYNKILSVLIKYGFDDYVAYLEENHRFPLIR
                     KIISRQRFKQAVNYSKWEKMRKVCEELGPTFVKFGQIISNRPDLLPDDLINELKKLQD
                     SVPPFPYKQVEEILHKEFGKPVEKLYASFDPTPIASASMAQVHKAVLHDGTIVAVKVQ
                     RPGIKVMISQDILIMRDIAKMLARRIPSLKQFDAEGLVEAFADSIQKELDFIHESWNL
                     QRFRHQFREDPEVYVPNYYKELSSGKVITQDFIFGVKISDLEKIKAQNLDPKILAKRT
                     LSSFYRQLFDYGFFHADPHAGNIIAQENNIVAYLDFGMMGSIMKRDMENLGNLILAIE
                     EKNVKRIIRYIHFLGDISIINDSRQLEFDVHEFVGKYSAAQSEKYQDNISDILLELKD
                     IIIKHNLKVPAHFFLLTRAMVSAEGIVRQLDPELSLTDEIRPYIISMIKKDHGLVSFG
                     RKFYNFANEFSSYMEDFPNDLRQVMRMAKNGQFKVDLQHKGVDPMIYTMEKVSRQMVL
                     TILIASFLVGSALLVVSQVPPLWYGMSAWAWFGFGVVFVLLVLMISSLSNYYRPEKED
                     "
     gene            complement(4443..4739)
                     /locus_tag="PK610_07100"
     CDS             complement(4443..4739)
                     /locus_tag="PK610_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35737.1"
                     /translation="MEKDIIKNLMYMAVGYASMNRERIEEFAKELTAKGKMTEEEGKK
                     MIQELIDRSKQVKEDIENKIENVSKEIYNSLHIATKDEMEEIRKRLSAIEEKLK"
     gene            4910..5677
                     /locus_tag="PK610_07105"
     CDS             4910..5677
                     /locus_tag="PK610_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRJ35738.1"
                     /translation="MSTIVCPVDFREPSFQAARIAAIIAQKSDAQLLFLHSAGSESDE
                     ARERVFTGMERLGFHVGSAQMKEPQFVVSPEGLSDSINALNHKKDIGLVVMATDGISN
                     LTELSAGTHTENVASKTNVPVLAIPAGAKVGTFNSILFATDYEKTSKADIESLLLFAK
                     IFNATVEIIHVSRDENPVSKGVFEIFSNDVRSLFGNDVTITRVIADDVAAVMIQLSVE
                     KNIDLMVVHKDKFQVNDRSKTSTLLRMAEFPLLVLRS"
BASE COUNT         1508 a         1281 c         1249 g         1720 t
ORIGIN      
        1 tttcatcctt gaaatagcca tgcatggttc gtcctttgat ctggttatag caaagtgaat
       61 cggcaaatga agagatgaaa caatttccga tgagatcgat gcgatgcaat tctttatttc
      121 tgaaatgcac ctccatacgt tgtgcactta attgctgatc atcctgccag attaccggtt
      181 tgccgagcag gttcagcacc gaatcttttt catggtaatc gagcgtgtcg cacatgccct
      241 gaacatcatt tctgaaaaaa cggacatttc gccacgcacg catccactga gtgctgtctt
      301 taccttcgcg tgaagcaaat aaagtatctg catgaagata cagggtgtca tcgtcgaaag
      361 gtcgcatcat taatgcgctg tcggttacga atgcagattt taatttttca ttgaaccatc
      421 cgtattttcc ggagagtgtt gatttgttag ttgtgtcgta aagcgaaaaa aatccgcgtg
      481 ctgtacccag tcctgatttc tgcgaataat cgagcgtatc gccgcgaaat ttgtattcgc
      541 cgttactcat ataaacattt ttgataaaac gcgattgatc ttttcgtgta ttgtaccatc
      601 ctccttcgca gaggatcaca ccatcgttgg tgcgcaaaac ggtaggacca taaaaccatg
      661 cggtttccgt tggtgtatga tagcgaagtg tatcgcaggt catggtgtaa tccggatgtt
      721 tcagcataac ttctccggag aaaaaaatat ccttgcttgc cgatcggtat tcaccaaatt
      781 tgctcgtgag aacattttta ttttttgcag agcgaataat tcctcctgta gaataccatc
      841 cgcgtccttc cttcgaaaca aattcaagcg aatcggtttc catgctcata tcgagctcca
      901 ccattcgaat atgtccgcgt aattttccgt agccggtatt gccatcgtaa aaaagagaat
      961 cgccgtacat attcagactg tcgccctgtt tcacatggag gtgtccgaac gcaaatagaa
     1021 cattacgctt ctggtagatc catgcgctat cgcaatacat gagggtgttg tcgtgacgca
     1081 cctgcacatt acccagtgcg cgctgggcat cgcctttgcg ttgatcgaaa atgactttgt
     1141 cggcattgag taactcaaca tttgccggtg gaggattctt tcctttggtt cctgtgtttt
     1201 gtgcatagct gattgatccg ccaaaaagca ggaaggttat caggatatgc agtgatttga
     1261 tcaaggttta acaacgctgg tttaggtatt atcgcatatt tcgttccaaa aaaatatcct
     1321 gcccttgcgg agcaggatat tcggtgttct gtttttttac ttaccgagtt tggctttcag
     1381 acctttgtcg atctcattaa ggaaatcttc ggtatagagg tagttatcgg cagtgacttt
     1441 atctccgtaa atacatactg cgaggtcttt cgtcattttt ccgctttcaa cggtttctac
     1501 acatactttt tcgagtgcat ccgcaaagtt gatcagctcc tggttgttgt cgagcttacc
     1561 acggaaggcc aatccgcgac tccatgcgaa gatggatgcg ataggattgg ttgaagtgcg
     1621 attacctttc tggtgttcgc ggaagtggcg tgtaactgta ccgtgtgcag cttctgcttc
     1681 catcactttt ccgtcgggag ttaccaatac agaagtcatc agtccaagcg aaccaaatcc
     1741 ctgtgcaacc gaatcagact gaacatctcc gtcgtagttc ttacaggccc acacaaattt
     1801 tccactccat ttcatggcac tggccaccat atcatcgatg aggcggtgct cataggtaat
     1861 tccggctgct ttgaatttgt ctttgaattc tgcatcgtac acttcctgga aaatatcctt
     1921 gaagcgtccg tcgtattttt tcagaatcgt attctttgta gaaaggtaaa gcggccatcc
     1981 ttttacaagc gccatgttca tacatgcact ggcaaatcca cgaatggatt cgtcgacatt
     2041 gtacatgctc aatgcaactc ctggtccgtt gaaggaatat acttcctgct caatcacagt
     2101 tccgtcctca ccggtgaatt tcaccatcag ttttcctttg ccgggtacca cgaaatccgt
     2161 agcacggtac tgatcaccga aagcgtgacg accaacaatg atcggagaat cccaggtagt
     2221 aaccagacga ggaacatttt tacatacgat cggctcgcgg aatactgttc cgtcgaggat
     2281 attgcggata gtaccattcg gcgacttcca catttgcttc aagttgaatt cctttacacg
     2341 ttcttcgtcg ggagtaattg tagcacactt gatcccaaca ccgtgttgtt taatggcatt
     2401 tgcagcatca atggtaacct gatcgttggt ggcatcacgg ttctccattc caaggtcgta
     2461 gtacttgata tccacgtcga ggtaaggaag gataagtttg tccttgatga atttccagat
     2521 gatccgggtc atctcatcac cgtcgagctc tacaacggga ttggctactt tgattttgct
     2581 catggttaaa tcgttttgtg agggtggcca aaaataactt ttttcacagc aaaacaagag
     2641 ggaaattatt cacaattgtt gggggatggt gaacagggaa cagtaaccta ttactcttat
     2701 cccctaactt ataacaaata tctattctgc catcaatcct ctttttcggg acggtaataa
     2761 ttggaaagtg aggagatcat taagaccagc aacacaaata ccaccccaaa gccaaaccag
     2821 gcccaggccg acattccgta ccatagtggg gggacctgcg atactacaag cagtgcagag
     2881 cctaccagaa aggaagcaat gagaatagta agaaccatct gccgggatac tttttccatg
     2941 gtgtaaatca taggatccac gcctttgtgc tgcaggtcga ccttgaactg accgtttttt
     3001 gccatgcgca taacctgacg aagatcgttg gggaaatctt ccatgtagct gctgaactca
     3061 ttggcaaaat tgtagaattt gcgtccaaat gaaaccaggc cgtgatcttt cttgatcatg
     3121 ctgatgatgt agggacgaat ttcatctgtg agcgaaagtt ctggatcgag ctgacgaaca
     3181 attccttctg ctgataccat tgcccgtgtg agaaggaaaa agtgcgccgg aactttcagg
     3241 ttgtgtttga tgataatgtc ttttagttcc aggaggatat ccgaaatatt atcctggtat
     3301 ttttcgcttt gggcagcgct gtatttgcct acaaactcat gaacatcgaa ttcgagttga
     3361 cgcgaatcgt tgatgatgga gatatcgccc aaaaagtgaa tgtagcggat gatccgtttt
     3421 acattttttt cctcaatggc aagaatcaga ttgccgaggt tttccatatc gcgtttcatg
     3481 atggaaccca tcatcccgaa atcgaggtag gcaacaatat tgttttcctg cgcaatgata
     3541 ttgcctgcat gcggatcggc gtggaaaaaa ccgtaatcaa aaagctgacg gtaaaaactc
     3601 gacaacgttc gttttgccag aattttcgga tcaagattct gtgcttttat tttttcaagg
     3661 tcgctgattt ttacaccgaa aatgaaatcc tgcgtgatca cttttcctga actcagttcc
     3721 ttgtaataat tgggaacata aacctccgga tcttccctga attgatgacg gaaacgctgc
     3781 agattccacg attcgtgaat gaaatccaat tccttttgaa tactgtcggc aaatgcttca
     3841 actaaacctt cggcatcaaa ttgtttgagc gaaggaatac gccgtgcaag catcttggca
     3901 atgtcgcgca taatgagaat atcctgagag atcatcacct taataccggg tcgctgcact
     3961 ttaacagcga ctatggtacc atcgtgcaaa accgctttat gcacctgcgc catcgaagcc
     4021 gaggctatag gagtggggtc gaagctggcg taaagttttt ctacgggttt gccgaattcc
     4081 ttatgcagaa tctcctccac ttgtttgtaa gggaagggtg gaacagaatc ctgtaacttt
     4141 ttcaattcat tgatcagatc atccggtaat agatcaggac ggttggagat gatctgcccg
     4201 aatttcacga aggtcggacc gagttcttca cagacctttc gcattttttc ccatttggaa
     4261 taattaacag cctgtttaaa gcgctgacgg gaaatgattt ttcggatgag agggaaacga
     4321 tggttttctt cgaggtaggc tacatagtcg tcgaatccgt acttgataag cacagataaa
     4381 attttgttgt agcgacgcgc atgcctgagc tgcctgaaga aaaatccgat cattcgggaa
     4441 aattacttca atttctcttc aatagcagaa agtcgcttgc gaatttcttc catttcgtct
     4501 tttgtagcaa tgtgaagact gttgtaaatc tccttgctga cattttcaat tttgttttca
     4561 atgtcctctt tcacctgctt ggaacgatca ataagttcct gaatcatttt tttgccttcc
     4621 tcttcggtca ttttgccctt tgcggtaagc tcttttgcaa attcttcaat gcgttcgcgg
     4681 ttcatggaag cgtatcccac tgccatgtac atcaggtttt tgataatatc tttttccata
     4741 attcaatttt ttacaaaggt gaaactctgt ttttccgcag cttatgacca gggtcatgat
     4801 tccagcggga taaactgata ctcatcaatt cccggcttaa aaggcaatcc caattttccc
     4861 tttcgcctga attgtggtat gttcgtacca aaatatgaaa attctcccaa tgagtacaat
     4921 tgtttgccct gtcgattttc gcgaaccttc ttttcaggct gcacggattg cagcaatcat
     4981 tgcccaaaaa agtgatgcac aacttttatt tctgcattca gcaggttccg aatcggatga
     5041 agcgagggaa cgtgtgttta cggggatgga acgtctcggt tttcatgttg gctctgctca
     5101 aatgaaagaa ccacaatttg ttgtttctcc tgagggactt tctgattcga tcaatgcact
     5161 gaatcataaa aaagatattg gtctggtggt tatggctacc gacggaataa gcaatcttac
     5221 agagctttct gccggaacgc ataccgaaaa tgttgccagc aaaaccaatg ttccggtgct
     5281 cgcaattcct gccggagcca aggttggcac attcaattcc attctctttg caaccgatta
     5341 cgaaaagacg tccaaagcag atatcgaaag cctgctcttg tttgcaaaaa tctttaatgc
     5401 aacagtagaa attatccacg ttagccgcga tgagaatccg gtttcaaaag gtgtattcga
     5461 gatattttcc aatgacgttc gatccctttt cggtaacgat gtgaccatca cccgcgttat
     5521 tgctgatgat gttgcagcag tgatgattca actttcggta gagaaaaaca tcgatctaat
     5581 ggttgtacac aaggataaat tccaggtgaa tgatcgttca aaaacatcca ctttgctccg
     5641 catggcagaa tttcctttgc tggtactaag aagctaatcg tttactgctt aatcagcagc
     5701 tgaatgggaa gccctaattt ctgacggaag atgttacctt cttcgtacat ttcttcat
//
LOCUS       DAOMYG010000181         5754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4772_length_5754_cov_10.282857, whole genome shotgun sequence.
ACCESSION   DAOMYG010000181 DAOMYG010000000
VERSION     DAOMYG010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4772_length_5754_cov_10.282857"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PK610_07110"
     CDS             complement(<1..252)
                     /locus_tag="PK610_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HRJ35739.1"
                     /translation="MSAKVRIAINGFGRIGRVFTRYLLEESNLELVAINDLTDSVTLA
                     HLLKYDSVHGRFPGEVVAGEKSIAINGRTIQVLAEKDPSM"
     gene            complement(327..3149)
                     /locus_tag="PK610_07115"
     CDS             complement(327..3149)
                     /locus_tag="PK610_07115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ35740.1"
                     /translation="MKNKNLLFSTISLGLLAGVVFNAASMANVSEKYTPRSAKGERPT
                     VDAAAEYYKSLRLNENGVYDEQLIKNTRDQVKAQLAAGEFARTANFHWYEEGPDNIGG
                     RTRAITIDRFFSGRVWAGSVSGGLFVSSNGGNTWGRVDSFDQTLAIASMGQTRGGRLF
                     VGTGFGGYFNEYFSGDGLFYSDDNGTTWIQVPSTSNFNWVEIIPDPVNPNKIWCATSN
                     GLKIVEGTSTLTVTNVAAPGGGQCFDVKVSPDGNVIVCAVASAGIRTWVSTDAGATFT
                     NVSGSGTGQIPASGVGRIEFAISFEKNSINNWNIYASSIKTNSTLFGQYFSDDNGLTW
                     AEIAPASSAGFDPFISANGQGIYNNVISAVPGRPDQALLGGIDVYAWNREQGLTPAFG
                     QWEQRSLWFASPLSPVYVHADNHEMEWDENNILYIGNDGGIGKSPDKGNLFFPANRGY
                     NVTQFYGIGFSADGDVIGGTQDNGTLLNRHTGVTMLTFEEVRGGDGFDCDISHMNPDV
                     MFASVYTGDMQRSDDGGFSFSPFFTPPTNAGFKTVGRLYENPKDFNSLETVGVQLGNS
                     TTLAGDILVPAGDTVWYVSKTFQTDLFYITPTNIVASTNGANIIQLQDYVQSLYAIAL
                     ASEVRITRQALRFSAAPSFTTVLTGEFAQCLEFSHDGNHLFVGTSDGDVWRISGLNTV
                     YAPVMDLSSVTTTRILQSAGASVEGIAIDMNDPEHVVITLSGYSSNRVRESTTAASTM
                     GTSSFSSIHGNLPNIPVYDAIINVNNPDQVIVGTEFGVYVTDNINATSTQWTFQSQVN
                     GPWLVPVYSVRQQWRKWEDGTNRPGEVYIGTFGRGIWRTEAFLSVKENDLNSSAARKI
                     LNVAIYPNPMQDNGRITFDLDEAANVNVSIYNISGQIVRSIALGNRPEGKLQVDVNVA
                     DLSNGTYIVRAQAGANVYQGRLVVTH"
     gene            complement(3360..4235)
                     /gene="lipA"
                     /locus_tag="PK610_07120"
     CDS             complement(3360..4235)
                     /gene="lipA"
                     /locus_tag="PK610_07120"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRJ35741.1"
                     /translation="METSEEGITVQNRQPKPKWLRVKLPTGESYKKVREIVSEHKLHT
                     ICESGNCPNMGECWGEGTATFMILGNVCTRSCGFCNVATGRPEAVDVFEPARVAQSVS
                     LMKVKHCVITSVDRDDLADGGSEIWAQTVNSIRRKSPETTLETLIPDFMGKWENLQRI
                     IDVKPEVVSHNLETVRRLTKQVRIQAKYDRSLEVLLRLKQAGLRTKSGVMLGLGETED
                     EILETIDDLISVQVDILTLGQYLQPSPKHLPVSEFVTPERFEKYREIALAKGFKYVES
                     GPLVRSSYHAEKHLF"
     gene            complement(4239..5000)
                     /locus_tag="PK610_07125"
     CDS             complement(4239..5000)
                     /locus_tag="PK610_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35742.1"
                     /translation="MSNLPIKPFVFLTSVLFVMPFLAWAQKPASEDIGLVYRKEVVGG
                     GILHSSGVGLHFRYGVQKSYKNKLSFGADFVNMRNPKEVKVSNPNYDDGRGYYYGKLN
                     GVMVLRPFFGERRILFQKLREQGVEVGYNWGIGPSIAMLKPVYLQVIYPTNSPFEMRT
                     VDERYDPSKHNVDNIYGRSRWGLGMDELKMRLGGHARFGLHFEFSPEEDRFKALEVGA
                     AFDLFPAKIPIMANELNKPYFFTLYLNFIFGRKHF"
     gene            5045..5452
                     /locus_tag="PK610_07130"
     CDS             5045..5452
                     /locus_tag="PK610_07130"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009780462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRJ35743.1"
                     /translation="MEISRIIYSGDLRTEATHLKSGKTITTDAPTDNNGKGDAFSPTD
                     LLATSLGSCMLTIMGIAASQHGFILGDIEVSIEKHMASAPRRVDKIVVTININEKGLS
                     EKDKKLLEASARACPVAKSLHPDLVQEVHFNYN"
     gene            complement(5443..5754)
                     /gene="ytxJ"
                     /locus_tag="PK610_07135"
     CDS             complement(5443..5754)
                     /gene="ytxJ"
                     /locus_tag="PK610_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HRJ35744.1"
                     /translation="MSDFDELVEKSVSDPSATFVVFKHSSRCSISDAAKGRMEREWES
                     VNPSVAVYFLDLITYRELSNYIASKIGVKHESPQLVIISKGKCIADYSHFEIRPSCIQ
                     L"
BASE COUNT         1759 a         1319 c         1132 g         1544 t
ORIGIN      
        1 catagaagga tctttttcag cgagcacttg aattgtgcgg ccattgattg caatactttt
       61 ctcacccgca acaacttctc ctggaaatcg tccatgaacg gaatcatact taagcaaatg
      121 tgcaagtgtt actgaatctg tcaagtcatt tatagcgacc aattcaagat tggactcctc
      181 aagcaggtag cgcgtaaaaa ctcttcctat tcggccaaaa ccattaattg caattcgaac
      241 ttttgctgac atgctgctta tttttccaaa tgtaaaacaa aaaaggccct gctattgtgc
      301 agggccttat actgaataaa ttaagattag tgtgttacaa ccaaacgtcc ctgataaaca
      361 ttcgcccctg cctgagcacg gacaatgtag gtaccattgg aaagatcagc tacgttgaca
      421 tcaacctgaa gcttgccttc aggacggtta ccaagcgcaa ttgaacgtac tatttgacct
      481 gagatgttgt aaatggaaac attcacattt gcagcttcgt cgagatcaaa cgtaatgcga
      541 ccattatcct gcattgggtt aggataaatt gcaacgttca agattttacg agcagctgaa
      601 gaattaagat cattctcttt cacactaagg aatgcttctg ttctccagat accacgtcca
      661 aaagtaccaa tgtaaacttc acctggacga tttgttccat cttcccattt gcgccattgc
      721 tgacgaacag aatagacagg taccaaccaa ggaccgttaa cctgactttg gaaagtccat
      781 tgggtagaag ttgcgttgat gttatctgtt acataaactc cgaattcagt tcccactatt
      841 acctgatcag gattgtttac gttaataatt gcatcgtaaa caggaatatt tggaaggtta
      901 ccatgaatag agctgaaaga agatgtaccc atagttgaag cggcagttgt tgattcccta
      961 acccggttag agctgtatcc acttaaagta ataactacat gttcagggtc gttcatatcg
     1021 attgcaatac cttcaacaga agcgccggca gactgaagaa ttctggtagt tgtaactgat
     1081 gaaagatcca taacaggagc atatacagtg tttaggccac tgattctcca aacatcacca
     1141 tcagatgtac ctacaaaaag gtgattacca tcatgactga attcgaggca ttgtgcaaac
     1201 tcaccggtta acacagtggt gaatgaagga gcagcagaaa atctgagcgc ctggcgagta
     1261 atacgtactt cagaagcaag agcgatagca taaagcgatt gaacataatc ctgaagttgg
     1321 ataatattag caccgtttgt tgatgcaaca atattggttg gcgtaatgta gaacaagtcc
     1381 gtttggaatg tttttgaaac ataccatacc gtatcacctg caggaaccaa aatatcgcca
     1441 gccagagtag tactgtttcc gagctgtaca ccaactgttt caagactatt gaagtctttt
     1501 ggattttcat aaaggcgacc aaccgtttta aacccggcat tagttggggg tgtgaagaat
     1561 ggcgagaaag agaatccgcc atcatcagaa cgctgcatat cgcctgtata aacagaggca
     1621 aacattacat ccggattcat atgtgaaata tcacaatcaa aaccatcacc accacgaact
     1681 tcttcaaaag ttagcatggt aactccggta tgacggttca atagtgttcc gttatcttgc
     1741 gttccaccga ttacatctcc atcagcagaa aaaccgatac cgtaaaactg ggttacgtta
     1801 tatccacggt ttgcagggaa aaacaggttt cctttgtcgg gtgacttacc aataccacca
     1861 tcattaccga tgtatagaat attgttttca tcccattcca tttcgtggtt atcagcatgc
     1921 acataaacag gcgacaaagg agaagcgaac cacaatgagc gttgttccca ctgaccaaaa
     1981 gcaggagtta aaccttgctc acggttccat gcataaacat cgataccacc aagcaaagcc
     2041 tgatcaggac gaccggggac tgcggaaatc acgttgttat aaattccttg tccgtttgcc
     2101 gaaataaaag gatcaaaacc tgcactggag gccggagcaa tctcagccca ggtcaaacca
     2161 ttatcatcag agaaatactg tccgaaaagc gttgagtttg tcttaatgct ggaagcgtag
     2221 atattccagt tattgatcga attcttttca aaagaaatag caaattcaat acgaccaaca
     2281 cctgaagccg gaatctgacc ggttcctgat ccggacacat tggtaaaggt tgcaccggca
     2341 tctgttgaaa cccacgtacg aatacctgca gaagctacag cgcaaacaat aacatttcca
     2401 tcaggagaaa ccttaacatc aaagcattgt ccgccaccgg gcgctgcaac attggtaaca
     2461 gtgagagttg aagttccctc aactattttc aatccattgc tggttgcaca ccagatttta
     2521 ttgggattaa ccggatcagg aatgatctca acccaattga aattagaagt tgaaggcacc
     2581 tgaatccagg ttgttccgtt atcatctgag taaaatagac cgtcccctga gaaatactca
     2641 ttaaaatatc ccccgaaacc ggttccaaca aacaaacgtc caccacgagt ttgacccata
     2701 gatgcaattg caagtgtttg gtcaaaactg tcaactcgac cccaggtgtt accaccattg
     2761 gatgaaacaa ataatccgcc acttacactt cctgcccata cacggccact aaagaagcgg
     2821 tcgatggtga tggcgcgggt gcggcctccg atattatccg ggccttcttc ataccaatgg
     2881 aaattggctg tacgagcgaa ctcaccagct gctaattgag ctttaacctg atcgcgggtg
     2941 tttttgatca attgttcatc gtaaacacca ttttcgttca aacgaaggga tttgtaatat
     3001 tcagccgccg catctacggt tgggcgttca ccctttgcag aacgcggggt gtatttttca
     3061 gatacattgg ccattgaggc agcgttaaaa accacaccgg caagcaagcc aagactgatt
     3121 gtcgaaaaaa gtaaattctt gttcttcata atcgcaacgt attactgcca ccttttctgg
     3181 caggcttaca aataaaacaa ctatttaagg aaatatcaac cccatttgag cgaaagggag
     3241 taaataatga gcagaaggta gccggtataa atccttgacc aggtaaacct gttgatttca
     3301 gaagctttcc gatctaattc agtccgtaaa aaataaggca attttataca agtctaagct
     3361 cagaagaggt gtttttcggc atggtaactg gatctgacta atggaccgct ttccacatat
     3421 ttaaatccct ttgccaatgc aatttccctg tacttttcaa atcgctctgg cgtaacaaac
     3481 tcagaaacag gcaaatgttt agggcttggc tgcaggtatt ggcccaatgt taggatatcc
     3541 acttgtacgc ttattaaatc gtcaatggtc tccagaatct catcttctgt ttcacccagt
     3601 ccgagcatta caccggattt tgttcgcaaa cctgcctgct tgagacggag taatacttct
     3661 aaactcctat cgtattttgc ctggatgcgt acctgtttgg ttaaacgcct taccgtttcg
     3721 agattgtgag aaactacttc cggtttaaca tcgataatgc gctgcagatt ctcccatttc
     3781 cccataaaat cagggatcaa tgtttcgagt gttgtctccg gacttttccg ccgaatcgaa
     3841 tttaccgtct gcgcccagat ttcagaccca ccatcagcta gatcgtcacg atcaactgat
     3901 gtaataacac aatgcttcac cttcatcaga gaaacactct gtgcaaccct tgcgggttca
     3961 aatacatcaa ccgcttccgg tctgccggtt gcaacattac aaaaaccaca agatcgggtg
     4021 caaacgttac caagtatcat aaacgtggcc gtaccttctc cccaacactc gcccatattc
     4081 ggacaatttc cgctttcgca aattgtatgc agcttatgtt cacttacaat ttccctgacc
     4141 tttttataac tttcgccggt aggtaatttt acacgaagcc attttggttt gggttgtcgg
     4201 ttttgcaccg ttattccctc ttcacttgtt tccatttctc agaaatgctt tcgtccaaaa
     4261 ataaagttca ggtacaacgt aaaaaaatac ggtttgttca gctcattcgc cataattgga
     4321 attttggcgg gaaacaaatc gaaagctgcg ccaacctcta atgctttaaa acgatcctct
     4381 tccggagaaa attcaaaatg aagaccgaat cgggcatgtc cgcctaacct cattttgagt
     4441 tcatccatac ccaaccccca gcgtgatcgg ccataaatat tgtcgacatt atgttttgag
     4501 gggtcatatc gctcatcaac agtacgcatt tcaaaaggac tattcgttgg gtatatcacc
     4561 tgaagataaa caggtttaag catagcaatg gatggaccta ttccccaatt gtaacctact
     4621 tcaacacctt gctcacgcag tttctgaaaa aggatccttc gctcaccaaa aaagggtcgc
     4681 aacaccatca caccattcag ctttccgtaa taatatccac gaccatcatc gtagttggga
     4741 ttcgaaactt ttacctcttt ggggtttcgc atattcacaa aatctgcccc gaaagaaagt
     4801 ttgtttttat aagatttctg aacaccatac ctgaaatgca aacccacacc actggaatgt
     4861 aatatgccgc ctccaacaac ttcttttcgg taaacaagac caatatcttc cgatgccggc
     4921 ttttgtgccc atgcaagaaa aggcatgaca aacaaaaccg aagtcaggaa aacaaatggc
     4981 tttattggta gatttgacat cacaacaaaa atacaactat tcaaccttaa agtttagtat
     5041 atcaatggaa atttcaagaa tcatttattc aggggacctt cgcactgagg caacgcatct
     5101 gaaatcagga aaaaccatta ccacagacgc tccaacagac aataatggca aaggggacgc
     5161 attttctcct acggatcttt tggcaacaag tttaggttca tgtatgctta caattatggg
     5221 aattgcagcg tctcaacacg gattcatcct tggggatatt gaggtaagca ttgaaaaaca
     5281 catggcatca gctccacgca gggtagacaa aattgttgta acgattaaca ttaacgaaaa
     5341 ggggctgagt gaaaaggata aaaaactact agaggcatcg gcaagagcat gtcccgttgc
     5401 gaaaagtctg catcctgatt tagtacaaga agtgcacttt aattacaatt gaatacaaga
     5461 agggcggatc tcgaagtgcg aataatcagc gatacatttt ccttttgaaa taatcaccaa
     5521 ttgaggtgat tcatgtttga ctccgatttt cgaagcaata taatttgaca attccctata
     5581 ggttataaga tcgaggaagt aaacagctac tgaagggttt acagactccc attcacgttc
     5641 catacgacct tttgccgcat ctgaaatgga gcatctggac gaatgcttaa atacaacaaa
     5701 agtggctgag gggtcagaaa cacttttttc aaccagttcg tcaaagtcac tgat
//
LOCUS       DAOMYG010000182         5754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4773_length_5754_cov_8.412178, whole genome shotgun sequence.
ACCESSION   DAOMYG010000182 DAOMYG010000000
VERSION     DAOMYG010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4773_length_5754_cov_8.412178"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /gene="rpsP"
                     /locus_tag="PK610_07140"
     CDS             complement(<1..188)
                     /gene="rpsP"
                     /locus_tag="PK610_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRJ35745.1"
                     /translation="MATKIRLARHGKKGKPVFHIVIADSRSPRDGRFIKKIGVYNPTS
                     NPATIDINFNEALAWLEKG"
     gene            998..2308
                     /gene="nhaD"
                     /locus_tag="PK610_07145"
     CDS             998..2308
                     /gene="nhaD"
                     /locus_tag="PK610_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HRJ35746.1"
                     /translation="MDIYAILMIVVFVVGYAAIALEHPIKVDKAASALLTGVLCWTLY
                     VFGGYTDHHGVELSLMHHLSEIASILFFLMGAMTIVELVDAHEGFSVITDRINTTNKI
                     TLIWAISFIAFFLSAALDNLTTAIVMISLLRKLIKDVETRWVFAGFVIIAANAGGAWS
                     PIGDVTTTMLWIKGQLPDVPSMIMHLFIPAIVCLIVPLLLAPIVLRKSTIERPVKVEE
                     LGHYTNPVTNREKYTVFLLGLGGLLFVPVFKTVTHLPPFMGMMLSLGVLWVVTEIMHG
                     SKNKEERRSVSVIGVLQKIDTASVLFFLGILLAVGALQVVGHLHTTAEFLDHALNKNI
                     YTINMSIGLLSAIVDNVPLVAAAQGMYEIAPEGLFAAGGSFWEFLAYCAGTGGSCLII
                     GSAAGVAVMGLEHISFGWYLKKIAPLALVGYIGGAITYILMFAF"
     gene            2383..4308
                     /locus_tag="PK610_07150"
     CDS             2383..4308
                     /locus_tag="PK610_07150"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ35747.1"
                     /translation="MKRPLLLVLGIISCSVWLLSAQENAKQFSIDEHLLKVGSHQQIR
                     AWGYQSNTLFSAAGICSQCHGYDIDQLASVDAMGNDVNVMDAWAGTIMANSAKDPLWK
                     AKVSHEVLVNPAHQNALESTCVKCHAPLGKYNALHLSQPYGMTELSTDSVGLDGVSCG
                     ACHQQRDTLIGKRFSGELFFTTDKTVYGPYTNPLGPMAGNTGFTPTYSPHINRAGLCA
                     GCHTLITQTSDLSGNLTGDHFIEQATYHEWLNSRFNNDIPNPQGISCQGCHIPRINDP
                     VKLSTGPPNLTRTPFGKHELVGANVFMLKLMKDNATALGLYSTARQLDSSIVRTERML
                     KQQSLSLTLQETSRTLDTAFYDLQLTNKAGHKFPSGYPSRRIYVEFVVRNDLGDTLFR
                     SGVRQNDELVNENPTYEPHYTMINSEQQIQIYEMVMGDVNDNVTTVLERGKSSLKDNR
                     LVPEGFTIGHPAYDTCKIIGGALSDPDFNRNGATEGTGADIIRYHIPLSGYTGALNVS
                     AKIYYESVPKRFLSEMFSYSSPEISLFQGMYNSSDKSPVLVAEALLIGGFQSVATVAS
                     PLLNIFPNPSTNGSVKFECGDKIDQIELFDLGGRRVRNIQPNSDAFFVDGSGLQGTYI
                     ARIRCGDHLLTRKIVFY"
     gene            4266..4967
                     /locus_tag="PK610_07155"
     CDS             4266..4967
                     /locus_tag="PK610_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRJ35748.1"
                     /translation="MRRSSAYPQNRILLMNPGEEAIIEQISQEIQSLFAGEGTGHDWH
                     HIERVFRLSLILAGKENADPFICALGALLHDIADHKFHPDDLGKNQRDAQAMMQKYAV
                     DQKSIQKVIQLIGEISYKGAGVATPVSTIEAACVQDADRLDAIGAIGIARAFAYGGSK
                     GRPLYDPDKKPVLHNDFDSYRKDEGHTINHFYEKLLLLKNRMNTTEGKRIADERHAFM
                     ELWMARFFGEWEGRC"
     gene            5000..5674
                     /locus_tag="PK610_07160"
     CDS             5000..5674
                     /locus_tag="PK610_07160"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRJ35749.1"
                     /translation="MKHLFSILLFTVVFFLPEVNAQTTRPATTATVKTDTTKQRKFIQ
                     FSGIIVTADSLRPIPFVNIFDKKTGRGTVSDYYGFYSFVAMEGDTIIFSSVGFKRSRF
                     VIPDTLTEDKYSLIQMMQPDTIVLPQVTVYPWPSKEQFAQAFVNMNLQDDYMARAYKN
                     MQDAQVRDAAIAMSNDAQMAYYQAMRMERYKLYYAGQVPPNNLLNPIAWYQFVQAWKN
                     GDFKQK"
BASE COUNT         1575 a         1248 c         1242 g         1689 t
ORIGIN      
        1 cctttttcta accaagccaa agcctcgtta aagttgatat cgatagttgc aggatttgaa
       61 gtaggattat aaactccgat cttcttaata aagcggccat cacgtggtga acggctgtcg
      121 gcgattacaa tgtggaaaac tggttttcct tttttaccgt gacgggcaag tctgatttta
      181 gttgccattt ttaattgttt taaaagggtc cgaaacccgg ttaattaata agtgaattgg
      241 gctgcaaaag tagaaaaaaa tacattcccc caaaggcttt agggtattta ttatttgcga
      301 caggtgcaaa aattaaaatt tcttaccaat aaattggaaa cgattctgat atagacttca
      361 tttgagcccg tgatcaggtt gtttcaaatt ggcttggtag tgattacctt ttggggttct
      421 gtttcgggac acccgcctgc ttttcaggaa attgccacct caacctacca gatttcccaa
      481 agcgattttt gtccggcagg tacccttttc aaagaagagg cctccgggtc tgccgctgtt
      541 tcattcgaat ccttttctcc cacctttccg ctgccagact ccaggttccc gggattgttg
      601 tcgtccgatg gaatctcaaa ggctttctgt cttcattcag aatctcaaca tccttcaatt
      661 gcaaaatcta ttttactggt taactgcgtt ttgcagatct gaagccctca catccggtag
      721 aattatgcgc cgaaaattgt cccggcaccg gatgcggaat gcatgcttaa tcattctgaa
      781 ggacctattt cctatcgcat taatttattt cccaatggag aacaacactt ccgaaggaaa
      841 gggatcgttt ttccttttcc tgatggttat tggagccatc atgaccttgc tttcgctgat
      901 agtcaattac ctcaattgaa agttcagccc tctgctttta tccaattaaa aaaagactaa
      961 tttcaccccg atttaaaaac cagaaaatcg aaatccaatg gacatctatg ctattttgat
     1021 gatcgttgtc tttgttgtag gatacgcagc cattgcgctg gaacatccta tcaaagtcga
     1081 caaggcggcc tctgcgttgc tcaccggcgt tttatgctgg acactttatg tttttggggg
     1141 atacaccgac catcatggtg ttgaactttc cttgatgcac catctttccg agattgcaag
     1201 tattcttttc ttccttatgg gagcaatgac cattgttgaa cttgtagacg cgcacgaagg
     1261 cttttcggta attaccgacc gaataaatac taccaataaa atcacgctca tctgggcaat
     1321 ttcatttatt gcctttttcc tgtcagccgc cttggataac cttacaacag ctattgtaat
     1381 gatatccttg cttagaaagc tgataaaaga tgttgaaaca cgatgggtat ttgccggttt
     1441 tgtgatcatt gccgccaatg caggaggtgc ctggagtccg attggtgacg taaccacaac
     1501 catgttatgg ataaaaggac aattgcctga tgttccttca atgatcatgc acctcttcat
     1561 tcccgccatt gtttgcctta ttgtacctct tttgctcgct ccgatcgtat tacgtaaaag
     1621 caccatcgag cgtcctgtta aagtggaaga actaggacat tataccaatc ctgtgacgaa
     1681 cagagagaaa tacactgttt tcctgttagg tttaggcgga ttactttttg tacctgtatt
     1741 taaaacggta acccaccttc caccttttat gggaatgatg ctttcattgg gtgttttatg
     1801 ggtggtaact gaaattatgc atggctccaa gaataaggaa gagcgtcgtt ctgtttcggt
     1861 tattggtgtt cttcagaaaa ttgatactgc atccgtactg tttttccttg gtattctttt
     1921 ggctgttgga gcacttcagg ttgttggtca tcttcacact actgctgaat tcctcgacca
     1981 tgcgctgaat aaaaacatct acaccatcaa catgtccatc ggtttgcttt ctgcaatcgt
     2041 agataacgtg ccgctggtag ctgctgctca gggaatgtat gaaattgccc ctgagggatt
     2101 atttgcagca ggtggaagct tctgggaatt ccttgcgtat tgcgcaggta ctggaggctc
     2161 ttgtttgatc atcggatcgg ctgcaggtgt tgctgttatg gggcttgaac acatcagctt
     2221 tggctggtac ctcaaaaaga ttgctcctct tgctcttgta gggtatattg gaggcgcgat
     2281 cacctacatt ttgatgttcg cattctaatc attttcaagc attaacgaac cccgtgacaa
     2341 acctcgccaa aggttttcac ggggttcttt atattagcag gtatgaaacg tccccttctg
     2401 cttgtattag gaatcatttc ctgttccgtc tggctgcttt ctgcgcagga aaatgcaaaa
     2461 caattttcca ttgatgagca tttattgaaa gtcggttcac accagcaaat acgtgcgtgg
     2521 ggctatcaga gcaatacttt attttctgct gcaggaatct gctcccaatg ccatgggtac
     2581 gatatcgatc agctcgcttc tgttgacgca atggggaatg atgtaaatgt tatggatgcc
     2641 tgggcaggaa ccattatggc taattcggca aaagatccgc tttggaaagc aaaagtgagt
     2701 catgaagttt tggtcaatcc ggcacaccag aatgcgttgg aatcaacttg tgtaaagtgt
     2761 catgctccat tgggaaaata caatgcatta cacctgagtc agccttatgg aatgaccgaa
     2821 ttaagcacag attctgttgg gctggatggt gtttcatgcg gtgcctgcca tcaacaacgc
     2881 gatactctta tcggtaaacg tttttccgga gaattgtttt tcacaaccga taagacagtt
     2941 tacggtcctt ataccaaccc tttaggacca atggccggaa atactggatt cactccaacc
     3001 tacagtccac acatcaacag agccggttta tgtgccggtt gtcacacact cattacgcaa
     3061 acatccgacc tcagcgggaa tttaacgggc gatcatttta ttgagcaggc tacgtatcac
     3121 gaatggctga attcccgatt taacaatgac atccccaatc cacagggtat ttcctgtcag
     3181 ggatgtcata ttccccgaat caatgatcct gtgaaacttt ctacaggtcc gccaaacctt
     3241 acacgaacac cattcggaaa acatgaattg gtcggtgcaa atgttttcat gctaaagctg
     3301 atgaaagata atgcaactgc gcttgggtta tactcaacag caagacaact cgattcaagt
     3361 attgtacgca cagaacgaat gctgaaacaa caaagcctct cactgacctt gcaggaaaca
     3421 tcccgtactc tggataccgc attttatgat ttgcaactga ccaacaaagc aggacataaa
     3481 ttcccaagcg gatatccttc acgtagaatt tatgtggagt ttgtggtaag gaatgatctt
     3541 ggagatacgc tattccgttc gggtgttagg caaaacgatg aattggtaaa tgaaaatcca
     3601 acgtacgaac cacattatac catgattaac tctgaacaac aaattcagat ttacgaaatg
     3661 gtaatgggtg atgtgaacga taatgtaact actgttctgg aaagaggaaa atcatcgctt
     3721 aaggacaacc gtttggtacc tgaaggattt acaattggac atcctgctta cgatacctgt
     3781 aagatcattg gaggtgcact tagcgatccg gattttaacc gcaacggagc aacagaagga
     3841 accggcgccg atattatacg ataccatatt ccactttccg ggtatacagg agcattaaat
     3901 gtgagcgcaa aaatttatta tgaatccgtt cccaaacgat ttctgagtga aatgtttagt
     3961 tattcctcac ccgaaataag tctgtttcag ggaatgtaca acagttccga caaatcgccg
     4021 gtattggttg cagaagcatt gctgattggc ggatttcagt ctgtagcaac ggttgcatct
     4081 cccctactta acatcttccc gaatccaagt acaaacggaa gtgtaaaatt cgagtgcggt
     4141 gataagatcg atcagattga actatttgat cttggtggaa gacgtgttag gaatattcaa
     4201 cccaacagtg atgcattttt tgtagatggg tccggattgc aaggcactta tattgccaga
     4261 atacgatgcg gagatcatct gcttacccgc aaaatcgtat tttattgatg aatccgggtg
     4321 aagaggcaat aattgaacag atcagtcaag agatccaatc actttttgca ggagaaggaa
     4381 ctgggcacga ctggcatcac atcgaaaggg tatttcgctt gtcgctaatt cttgcgggta
     4441 aagaaaatgc cgatcctttt atttgcgctc ttggtgctct gctgcacgat attgccgatc
     4501 acaaatttca tcccgacgat cttggaaaaa atcagcgtga tgcgcaagcg atgatgcaaa
     4561 aatatgctgt cgatcaaaaa agcattcaaa aggtcattca acttattgga gaaataagtt
     4621 acaagggcgc tggtgttgcc actcctgtaa gcaccattga agcagcgtgt gttcaggatg
     4681 ctgatcggtt ggatgccatt ggcgcaattg gaattgcccg ggcattcgca tacggaggat
     4741 cgaaaggtcg tccattatac gatcccgata aaaaacccgt tctacacaat gattttgact
     4801 cctaccgaaa agatgaaggt catacgatta accattttta tgaaaaattg cttttgctca
     4861 aaaatcgcat gaacacgact gagggaaagc gaattgctga tgaacgtcat gcctttatgg
     4921 aactctggat ggcacggttt ttcggggaat gggagggtcg ttgctaaaaa agggcctatc
     4981 tttgcaaaac agattgaaca tgaagcatct attctccata cttctattta ccgttgtgtt
     5041 tttcctgccg gaagtcaatg cccagacaac acgacctgct accacagcca cggtaaaaac
     5101 agataccacc aagcaaagga aattcattca attttccggt attattgtca ctgcagatag
     5161 tctgagacca attccctttg taaatatttt cgataagaaa acaggacgcg gaacggtttc
     5221 ggattattat ggattctact ctttcgttgc gatggaagga gacaccatca tattttcttc
     5281 agttggcttt aaacgttctc gcttcgtaat tccggatacg ttaacggaag acaagtattc
     5341 tctaattcag atgatgcaac ccgatacaat tgtcctgcct caggtaacag tgtatccctg
     5401 gccttcgaaa gagcaatttg cgcaggcttt tgtgaacatg aatcttcagg atgattacat
     5461 ggctcgtgcc tataaaaaca tgcaggatgc acaagtccga gatgcagcta tcgccatgag
     5521 taatgatgca caaatggcct attaccaggc tatgcgcatg gaacgctaca aactctatta
     5581 cgccggtcag gttcctccca acaatctgtt gaatccaatt gcctggtatc agtttgttca
     5641 ggcctggaag aacggggact tcaaacagaa gtaaattcgg ctttttcagc ttattacccc
     5701 taatcctcat ttgaggttta tttcactttt ttgcccctcc tttttttcgt gagg
//
LOCUS       DAOMYG010000183         5723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4808_length_5723_cov_10.058222, whole genome shotgun sequence.
ACCESSION   DAOMYG010000183 DAOMYG010000000
VERSION     DAOMYG010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4808_length_5723_cov_10.058222"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1833)
                     /locus_tag="PK610_07165"
     CDS             complement(<1..1833)
                     /locus_tag="PK610_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35750.1"
                     /translation="MQRILFVLVFLPSMLMAQIGPLGSWRDHLPYDRSIRVAAAGTKV
                     FCTTGSALFAFDADDNSIQRFSKANLLSDVAIRSMAYDAASASLVVGYANGNLDLIHV
                     PTLSKYNLTDIKRANIVGDKSIYHIHTENGKAYLSTGFGVVLIDIIRQEVKDTYFLSS
                     SGNRKVNAVTVHNDTVYAATDKGLYRGLATNPFLSDPNSWSKVLQFPTASRDSVFSDV
                     VLFNGEVYALFSSQGFTRDTVYRHTGNFWSPFVPLNGREIRSLQSSGGLLLASMHDAV
                     ESFDASLNQVDNIFGYTFGNPSPMHTVRSNGFYWIADNRLGLVRAINPWENNSIVPSG
                     PRSASSFDIDIEDGNVWVAPGFVYGAAWLNSYNSDFISGKVDDDWQTRAHMSDPLNLL
                     GQDSLFDVMAVAVDPNDPTHVFAGSLSFFGLIEVKDKVLVASFDEKNTPLPFMVGRPG
                     YCGITTLEYDKDGNLWMGVAYADRPLAVRKKNGTFVSYDLGSVIDNRVYRRMVVGKRS
                     GIKWVAIPAGNLSGGLFAWDDKSTMDDPTDDFYYFFGTGAGNGNLPSADVFAVAEDLD
                     NEIWIGTGAGIAVMYNQEAFSSGGNFDAQQILIQQDGNYQYLLET"
     gene            complement(1978..2853)
                     /locus_tag="PK610_07170"
     CDS             complement(1978..2853)
                     /locus_tag="PK610_07170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002679595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ35751.1"
                     /translation="MLTISRCPVCDGQSFPPFLTCKDFTVSEESFPIVSCSDCGFLFT
                     NPIPDLDVLGNYYKAESYVSHTSSSKGLINFLYQRVRKITLKRKLRLIQRIAKGNRIL
                     DFGCGTGHFLARLQAAKFDVTGVEPDADARAVAEKLNGIRPLQRHELSSLQPGFDVIT
                     LWHVLEHLPDLNKDISELIALLAPGGTLLVAVPNPTSGDAELYKEYWAGYDVPRHLYH
                     FSPTVIKRLFEKHGMECSACLPMPFDAYYVSMLSEKHKGGGMLRGFFNGWKSNRRARK
                     GGDKWSSQIYLFQKK"
     gene            complement(2887..4758)
                     /gene="mnmG"
                     /locus_tag="PK610_07175"
     CDS             complement(2887..4758)
                     /gene="mnmG"
                     /locus_tag="PK610_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRJ35752.1"
                     /translation="MLPDYDIIVVGAGHAGCEAAAAAANMGSKVLLVTMNMQTIGQMS
                     CNPAMGGIAKGQIVREVDAIGGYSGIVSDRSAIQFKMLNRSKGPAMWSPRTQNDRMLF
                     AQIWREMLEQTPNVHFWQDMVSGLIVENNCIKGIRLGMGLEVRAKAVVLTNGTFLNGL
                     IHVGEKQFGGGRMGEKAATGITEDLLQLGFESGRMKTGTPPRIDGRSLDYSKMEEQPG
                     DENPGRFSYTSTTPLDRQVSCYMTYTNDIVHDILRSGFDRSPMFNGAIQSTGPRYCPS
                     IEDKVNRFAQRERHQIFVEPEGWNTVEIYVNGFSTSLPEDVQYSALKQIPGFEKALMF
                     RPGYAIEYDYFPPTQLKHTLETKLVSNLYFAGQINGTTGYEEAACQGLMAGINAHLKI
                     NEKEPFILQRSEAYIGVLIDDLITKGTEEPYRMFTSRAEFRILLRQDNADLRLTPRAH
                     ELGLASSARLKAVERKLSETKQIVQFFKKESVDPIEVNSRLEELGTDPIRQKVKLHSV
                     LMRPQVAFDDLARHCLPLKHFLETGGPFEQESIEQAEIQMKYEGYIEREEEVANKMLR
                     LEDIGLPDDLDISRLSSLSAEAREKLVSIKPRTLGQASRISGITPADISILMIYLGR"
     gene            complement(4778..5203)
                     /gene="ybeY"
                     /locus_tag="PK610_07180"
     CDS             complement(4778..5203)
                     /gene="ybeY"
                     /locus_tag="PK610_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRJ35753.1"
                     /translation="MADTIQFHSLLTGFKLSNKSTISSWIDNAIREEGFKPGPISIVL
                     CSDEYLLEVNKMYLKHDYYTDIISFDYTDNQIVSGDLLVSVDRVAENALEMGIDPYDE
                     LDRVFIHGVLHLCGYKDKTSKDQQVMRKRENHYLQKRNM"
     gene            complement(5209..5634)
                     /locus_tag="PK610_07185"
     CDS             complement(5209..5634)
                     /locus_tag="PK610_07185"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ35754.1"
                     /translation="MEQAYASPVKEIHFPSRIENLTLVEKMIDEVCEQFKVNQDHYGN
                     ILIALTEAVNNAILHGNKQDPGKNITLCFENKDQDLLFMVQDEGNGFNFQDLPDPTDP
                     ANIEKPNGRGVFLMRNLADEVTFSSDGRRVQLRFKAFAN"
BASE COUNT         1632 a         1387 c         1202 g         1502 t
ORIGIN      
        1 tgtttcgagt aaatactgat aatttccatc ctgctgaatc aggatttgct gggcatcaaa
       61 atttccccct gaagaaaatg cctcctggtt atacatcact gcaattccag ctcctgtacc
      121 tatccaaatc tcattatcca gatcttcggc aacagcaaac acatctgcac ttggaagatt
      181 tccattgccc gcacctgtac caaaaaagta atagaaatca tctgttggat cgtccatggt
      241 cgacttatca tcccaagcaa aaagacctcc actcaaatta cctgcaggaa tggcgaccca
      301 tttaatccct gaacgcttgc caactaccat gcgtcgataa acccggttat cgatgacaga
      361 gcccaggtca taagacacaa atgtgccatt cttcttccgc actgcaagag gtctgtcggc
      421 ataagcaacg cccatccaca agttgccatc tttatcgtat tcaagtgttg ttattccaca
      481 ataacccgga cgtccaacca taaatggcaa tggtgtgttc ttttcatcga aggacgccac
      541 aagcacttta tcctttacct caatcaatcc aaaaaacgaa agagatcctg caaacacatg
      601 agttgggtca ttcggatcca cagcaacggc cattacatca aaaagagaat cctgtcctaa
      661 caaattcagg ggatcactca tatgagccct tgtctgccag tcatcatcaa cctttccaga
      721 aataaaatcg gagttatagg aattaagcca ggccgcgcca tacacaaaac ccggagccac
      781 ccaaacattt ccatcttcaa tatcgatatc gaacgaagaa gcagaccgtg gtccggaagg
      841 aacaatgcta ttattttccc aagggttaat agcccgaacc aaccccaggc gattatcggc
      901 aatccaatag aaaccattcg aacgaacagt atgcattggc gatgggttgc cgaaggtata
      961 tccgaaaatg ttatcgacct gattgaggct tgcatcaaac gattcaaccg catcatgcat
     1021 agaagcaagc aggagtccgc ctgatgattg cagtgaacga atttcccttc cattaagcgg
     1081 aacgaaggga gaccagaaat ttccggtgtg acggtatacc gtgtcgcggg taaaaccctg
     1141 tgagctgaac aaggcataga cctcgccgtt aaaaagtacc acatcgctaa aaacagaatc
     1201 gcgggaagca gtggggaact gcaaaacttt ggaccaggag ttcgggtcgc tcaaaaaggg
     1261 attagtggca agtccccgat acaaaccttt atctgtggct gcataaacag tgtcgttgtg
     1321 caccgttacc gcattcacct ttctgtttcc ggagctcgat aaaaagtacg tgtccttaac
     1381 ctcctgccgt atgatgtcga tcaacacgac tccgaatccg gtcgataaat atgcctttcc
     1441 gttttccgtg tggatatggt aaatcgactt atccccaaca atatttgccc ttttaatatc
     1501 tgttaggttg tacttgctta aggtaggaac gtggattaaa tcaaggtttc cgtttgcata
     1561 tcccacaacc aaagaagcag aagcagcatc ataagccatg ctgcgaatag caacatcaga
     1621 aagcagatta gccttgctaa aacgctggat ggaattgtca tctgcatcaa atgcaaacaa
     1681 ggcagaaccc gttgtacaga acactttggt tccggcagca gcaactctta ttgaacgatc
     1741 ataaggcaaa tgatcccgcc atgaacccaa agggccaatc tgagccatga gcatggaggg
     1801 caggaaaacg agtacaaaaa ggatccgttg catggtgtaa aatgcttttc agaacaacga
     1861 aattacctgt gttttggtat gaaaaaagcc ccaaaggccc tgctgtgaag aagtattgaa
     1921 aactcgaaaa cataaaaaaa gcgcagaaaa actgcgcttc aacggatgaa aatgatccta
     1981 ttttttctgg aaaagataaa tctggctcga ccatttgtcg ccaccctttc gggctcttct
     2041 gttcgatttc cagccgttga agaatcctct gagcattcct cctcctttgt gtttctcgct
     2101 taacatggaa acataataag catcaaaggg catgggtaaa caagcgctgc attccatgcc
     2161 atgtttctca aaaaggcgtt ttattacagt aggtgaaaaa tgatagaggt gacgcggaac
     2221 atcataaccg gcccaatact ccttgtacaa ttcggcatcg cccgaagtag ggtttggaac
     2281 agcaacgagc aaagttccgc cgggcgccag aagcgcaatc aactcgctga tgtctttgtt
     2341 cagatcaggc aaatgttcca acacatgcca taaagtaatc acatcgaagc ccggttggag
     2401 ggaggaaagt tcgtgtcttt gaagaggacg aataccattc aacttctctg ccacagcacg
     2461 cgcatcggca tcgggttcga cccctgttac gtcaaacttc gccgcttgaa gccttgctaa
     2521 aaaatgtccc gtcccacagc caaaatcaag gatccgattt ccttttgcaa ttctttgaat
     2581 cagccttaat ttccttttaa gcgtgatttt gcgtacccgc tgatacagga aattaatgag
     2641 cccttttgaa gaactcgtgt gcgaaacata gctttcggct ttgtaataat tccccaaaac
     2701 atccaggtcg gggataggat tggtaaagag aaacccacaa tcgctacacg aaacaatagg
     2761 gaaagattcc tcgcttacgg tgaagtcttt acaggtaaga aaagggggaa aggattgccc
     2821 atcgcataca ggacagcgag aaatggttag cattcggaat gtttcacgtg gaacaaaggg
     2881 acagttttac cgccccaaat atatcatcaa tatggatatg tcggccggtg taatgccgct
     2941 aatacggctg gcctgaccca aagtgcgggg cttgattgaa accaactttt cacgcgcctc
     3001 cgccgataag gagctcaaac gggaaatgtc caaatcatcg ggtagaccga tatcttcgag
     3061 acgaagcatc ttgtttgcga cttcttcttc acgctcaata tagccttcgt atttcatctg
     3121 aatctcggct tgctcgatgg actcctgctc aaaaggaccg cctgtttcaa ggaaatgttt
     3181 taggggcaaa caatgccttg caaggtcgtc gaaggccacc tggggacgca tcaataccga
     3241 atgaagcttt accttttgcc ggataggatc ggtgcctaat tcttccagtc ttgaattaac
     3301 ttcaatggga tcgacgcttt ctttcttgaa aaactgaaca atttgtttgg tctccgagag
     3361 cttccgttca accgctttca ggcgcgctga agaggcaaga cccagttcat gtgcccgcgg
     3421 ggtaaggcgc aaatcggcat tatcctgtcg caacaatatc ctgaactctg ccctggaagt
     3481 aaacatgcgg taaggctctt ctgtgccctt tgtgataagg tcatcaatga gaactccgat
     3541 atatgcctcc gagcgttgga gtataaaagg ttccttttcg ttgatcttta aatgcgcatt
     3601 tattcccgcc atcaagccct gacatgccgc ttcttcatag ccggttgtcc cgttaatctg
     3661 tccggcaaaa tatagattac ttaccagctt cgtttctaaa gtatgcttga gttgggtagg
     3721 ggggaagtag tcgtattcga tggcataacc cggacgaaac atcaaggcct tttcgaagcc
     3781 cgggatttgt tttaatgccg aatactgaac atcctcaggc aaggaagtcg aaaatccgtt
     3841 tacgtaaatc tccacggtat tccatccctc cggctccaca aaaatctggt ggcgctcacg
     3901 ttgagcaaag cggtttacct tgtcttcaat agaaggacaa taccttggcc cagtgctttg
     3961 aatggctcca ttaaacatgg gtgaccgatc aaatccacta cgaagaatat catgaacaat
     4021 gtcattggtg taggtcatgt aacacgacac ctgacgatcg agcggggtag tagaagtata
     4081 ggaaaaacga cctgggtttt catcgcctgg ttgttcctcc attttagaat aatccaaact
     4141 tcgcccatcg atacgaggag gtgtaccggt tttcattcta cccgattcaa atcctaattg
     4201 caatagatcc tctgttattc cggtcgctgc tttttcaccc attcttccac cgccgaattg
     4261 cttttccccg acatggatca atccatttaa aaacgttcca ttggttagta ctacagcttt
     4321 ggcccgaact tcaagtccca tgcccagacg gatgcctttt atacaattat tctctactat
     4381 taatcctgaa accatgtcct gccagaaatg gacgttggga gtttgctcca acatttcgcg
     4441 ccaaatctgt gcaaacaaca tgcgatcatt ttgagtgcgc ggactccaca ttgcaggccc
     4501 ctttgaacga ttgagcattt tgaactgtat ggcagagcga tcagaaacaa ttccggaata
     4561 gccaccaatc gcatcgacct cacgaacgat ttgacccttt gcaattccac ccatggcagg
     4621 gttgcaagac atttgtccga tggtctgcat gttcatggtc accaacaaaa ccttcgaacc
     4681 catattggca gcagcggctg cagcttcaca cccggcatga ccggcaccaa caactataat
     4741 atcgtaatca ggcagcataa ttgttccacg tgaaacatta catgtttctc ttttgtaaat
     4801 aatgattttc tctcttgcgc atcacttgtt gatctttact tgtcttatcc ttgtatccac
     4861 aaaggtgtaa cacaccgtgg ataaatacgc ggtcaagctc atcataagga tctattccca
     4921 tctccaatgc attttcagca acccgatcaa cggaaaccag caagtctccg ctaactatct
     4981 gattatcagt ataatcgaag ctaatgatat cagtataata atcatgcttt aaatacatct
     5041 tgtttacctc caaaaggtat tcatcggagc aaagcacaat tgaaatggga ccgggcttaa
     5101 atccttcttc ccggattgca ttatcaatcc aggaggaaat ggtgctttta ttggaaagct
     5161 taaaaccggt aaggagtgaa tgaaattgta ttgtgtctgc cactttgttt agttggcaaa
     5221 cgccttaaag cgcaactgaa ctcttctgcc atcactggag aatgttactt catccgcaag
     5281 gtttctcatc aagaaaacgc ctcttccatt tggcttttct atgttagcag gatcagtagg
     5341 gtcggggagg tcctgaaaat taaaaccatt gccctcgtcc tgaaccatga ataaaaggtc
     5401 ttgatcctta ttctcgaaac acaaggtgat gttcttaccg gggtcttgtt tattgccatg
     5461 aagtatggca ttgtttactg cttcggtaag cgcaatgagg atgtttccgt agtggtcttg
     5521 attgacctta aactgctcac aaacctcatc gatcatcttt tctaccagtg taagattctc
     5581 aatcctcgaa ggaaaatgaa tctcttttac cgggctagcg taagcttgct ccatttactt
     5641 ttcgattatg ttgaagtact cgttcacacg acctttataa taaggtgcta aagcaggagg
     5701 aacggtgcgc aaaagctcaa ctt
//
LOCUS       DAOMYG010000184         5707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4825_length_5707_cov_8.681175, whole genome shotgun sequence.
ACCESSION   DAOMYG010000184 DAOMYG010000000
VERSION     DAOMYG010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4825_length_5707_cov_8.681175"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..419)
                     /locus_tag="PK610_07190"
     CDS             complement(36..419)
                     /locus_tag="PK610_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009410371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HRJ35755.1"
                     /translation="MEEGYENNGNGRERDRDRDEIYSKAVRAGKRTYFFDVKTTRGSD
                     LYITITESKKRFDKDGLKVNYEKHKIFLYKEDFEKFVEGLNETVSKISELRENGYSGG
                     ELVQDNQVSNTNFNYTTVEFEDLDK"
     gene            complement(500..2287)
                     /locus_tag="PK610_07195"
     CDS             complement(500..2287)
                     /locus_tag="PK610_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010135794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ35756.1"
                     /translation="MRALKHLNAYFIRYKWMLLLGILFITISNYFGVRAPELIRYTFI
                     DIEKWISQGAEPGVDRNYIISQALTAALVYAGIYMLYALLKGLFLFFTRQTIIVMSRH
                     IEFDLKNEIYAQYQKLSLSFYRRNNTGDLMNRISEDVSRVRMYIGPAVMYITNLAVLF
                     VLVIYQMLAINPELTFYVLLPLPVMAFLVYTVSKRINKKSEEVQAQQSILTTHAQEAF
                     SGIRVIKTFAREDSIHNDFIKESKLYRSKVLSLVRTDALFAPVITLLIGLSTILTVYI
                     GCLKAFDGEVKVGVIAEFVIYVNMLTWPFATVGWVSSLIQRAAASQERINEFLHIEPE
                     IVNHNHNEMTIEGHLQFRNVSFTYPDSGIKALRAIDLEVKPGQTLAIIGKTGSGKSTL
                     ASLICRLYEATDGEVLLDKQDIRSANLDILRSSIGYVPQDVFLFSDTIANNIAFGLKQ
                     GSASQDKIVQSAKDACIHDNITGFKDGYETLVGERGITLSGGQKQRVSIARAIIREPR
                     ILIFDDCLSAVDTETEDQILKNLRRIMAGRTSVIISHRVSTVRHADKIIVLQQGSIIE
                     QGSHQELIDKKGYYFDLHRKQLLESQKIG"
     gene            complement(2381..3412)
                     /locus_tag="PK610_07200"
     CDS             complement(2381..3412)
                     /locus_tag="PK610_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRJ35757.1"
                     /translation="MKKITLEDIAKSLGVSKTLVSLILNNKAKEHGISPDTVDRVLKK
                     VKEMNYMPNQAARGLRTGKTNLIGLIVADISNPFYARISRAIEDLAWKKDYHLMVCSS
                     DEDPEKEAQLVRILREKMQVEGLIISPTTDNVEVFRDLKKIEFPFVLIDRNYPKFSCN
                     SVVVDNIRSSQSAVKHLIDSGAKRVALLTLSPAHISTLSERQEGYKLALKEAGIPFEE
                     DLVREVPFSDIKNSVKKTLQDWLKAKQPIDAVFCANNHLAVAVLETISDLGLRIPYDL
                     RMVSFDDVDVFKFSYPPVSAVAQPIEDIGTRAMEILFNNINDRASRESAEHVVLPAEL
                     VIRRSSSSL"
     gene            3681..4772
                     /locus_tag="PK610_07205"
     CDS             3681..4772
                     /locus_tag="PK610_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HRJ35758.1"
                     /translation="MEVKEIKTANADNPVISQMGMMGHEQVLFCQDEETGLRAIIAVH
                     NTVLGPALGGTRMWPYATEMEALTDVLRLSRGMTYKSAVAGLNLGGGKAVIIGDSRKD
                     KSEALFRRFGKFVNSLGGKYITAEDVGISPREMSWVNMETNHVAGLPGRSGDPSPVTA
                     YGVYMGIKAAAKEAFGNDSLNGKRVSVQGVGHVGEYLVGYLAKEGAKIFITDIHEETL
                     VRVANKYGATVVGTDEIYDLEVDVYAPCALGATVNDETLLRLKTPIIAGAANNQLKDE
                     KKHGEAVLAKGMIYAPDYAINSGGIINCYTEVKNLNNEWAMQNAENIYNTILSIIKES
                     KATNTPTYLVANRMAEQRIAAVGKLKRFM"
     gene            4812..>5707
                     /gene="nusB"
                     /locus_tag="PK610_07210"
     CDS             4812..>5707
                     /gene="nusB"
                     /locus_tag="PK610_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRJ35759.1"
                     /translation="MINRRHLRIKVLQALYSWFQSGDGDYGKSERELMKNIDKIYELY
                     LLYLLLLSDLQTIASNRIEDGKAKKLPTRQDLEPNLKFVENPVLELITKNKSLQAEVH
                     RRKLSWGLEMDMLKKIFQEIRNSDVFAKYMDDPGRNFVDAQEFVVTIFKDFIANSEAL
                     QNWLEEVNIHWLDDIDLVCAAVIKTIKGFQEKSDEFIRLLPLYKDPEEDEKFVKDLFR
                     KTIMNSTETEALISSKTDNWELERIASMDMILMKMAITEVSEFSTIPVKVTLNEFIEI
                     SKFYSTPKSNGFINGILDKVFA"
BASE COUNT         1620 a         1181 c         1219 g         1687 t
ORIGIN      
        1 aaagagacgg gttttagggg tgtaagtcga tttaattatt tatccagatc ttcaaactca
       61 acagtagtgt agttgaagtt ggtattcgat acctgattgt cctgaaccag ctctcctcct
      121 gaatagccgt tttcacggag ctcggaaatt ttggatacag tttcattcag tccttcaacg
      181 aacttttcaa aatcttcctt gtaaaggaaa atcttgtgtt tttcgtaatt gactttcagc
      241 ccgtctttat cgaaacgttt tttactctcg gtgattgtaa tgtacaaatc acttccacgg
      301 gtagtcttta catcaaagaa ataagtcctt tttccggcac gtaccgcctt ggagtagatc
      361 tcatcgcggt cacgatcgcg ctcgcgacca ttcccgttgt tttcgtaacc ttcttccatc
      421 tctggctctt ttggttgaac ttgatattgt tgcttgtgaa taccaaattt aacaactaaa
      481 gggacaaagc caaatttttt tatcctattt tttgagattc aaggagttgt ttgcggtgta
      541 ggtcgaaata atagcccttt ttgtcgatga gttcctggtg actaccctgc tcaataatgc
      601 ttccctgctg taatacaatg attttgtcgg catgtcttac agtagatacg cggtgactaa
      661 taatcaccga tgttcgtcct gccatgatcc gtcgcaggtt ctttaggatc tggtcttctg
      721 tttcggtatc tacagcggag aggcaatcat caaaaatgag gattcgcggc tcccgtatga
      781 tggcccgggc tatcgagact ctttgctttt gcccgcccga taaagtgatt ccgcgttccc
      841 caaccagggt ttcgtacccg tccttaaagc cggtaatgtt atcgtgaatg caggcatcct
      901 tggcagattg tacaatctta tcctgcgatg ctgatccctg ttttagtcca aatgcgatat
      961 tattggcaat cgtatccgaa aacaaaaaca catcctgtgg gacatagcct atcgacgaac
     1021 gaagaatatc cagattggcg gaacggatat cctgtttatc cagcaaaact tctccatctg
     1081 tggcttcgta aagcctgcaa ataagtgaag caagtgtcga tttccctgat cccgttttac
     1141 cgataattgc aagtgtttgt ccgggtttta cctccagatc aattgcgcgt aatgctttta
     1201 ttccgctatc gggataggta aagctgacat tcctgaattg caggtgacct tcaatggtca
     1261 tttcattatg attgtgattg acgatctccg gttcgatatg aagaaattca ttgatgcgtt
     1321 cctgcgaggc tgccgcgcgt tggatgagtg aactaaccca tcccacagtt gcaaagggcc
     1381 aggtaagcat atttacataa atcacaaact ctgcaataac accaacttta acctccccgt
     1441 cgaatgcttt taaacaaccg atgtaaacag taagaattgt acttaatcca ataagtaaag
     1501 taataacagg agcaaaaaga gcatcagtgc gtacaagact caacactttg ctgcggtaca
     1561 gtttgctctc cttgataaaa tcgttgtgaa tactatcttc tcgtgcaaag gtttttatta
     1621 cacggattcc tgaaaaggct tcctgtgcat gggtggtgag aatggattgt tgtgcctgaa
     1681 cttcttcgct ttttttgttg atgcgtttgc ttactgtata aaccagaaat gccattaccg
     1741 gaagcggcag aagaacataa aaagtaagtt ccggattaat agccagcatt tggtaaatga
     1801 caagaacaaa caatactgcc aggtttgtaa tgtacataac agcgggacca atatacattc
     1861 gcacacggct tacatcttct gaaatacggt tcatcaaatc tcccgtattg tttcttctgt
     1921 aaaaggataa acttagtttt tggtactgcg catagatttc attcttcaga tcaaattcga
     1981 tatgacgcga cataacaata attgtttgcc tggtaaaaaa cagaaacaat ccttttagca
     2041 atgcatagag catataaatt ccggcatata ccaatgctgc agtaagtgcc tgtgaaatga
     2101 tatagtttcg gtcaacaccc ggttctgcac cctgtgagat ccatttctca atatcaataa
     2161 aggtgtagcg aatcaattcc ggagcacgga caccaaagta attggagatg gtaataaaga
     2221 ggatgcccaa tagcagcatc catttgtagc ggataaaata agcgttaagg tgttttagtg
     2281 cgcgcatggg ggcggtaaaa ttaactaaaa aacaccttgc aggacttaat tgtttaagcc
     2341 ggattgaaga tgagctgcat tgagattaat taaacgtaat ttacaaacta ctgctggatc
     2401 ttctaatcac caattctgca ggtaaaacca cgtgttctgc tgattctctg cttgcacggt
     2461 cgttgatatt attgaaaagg atttccatgg cacgggttcc gatatcttca atgggttgag
     2521 caactgcact aaccggtggg taagagaatt tgaaaacatc cacatcatca aaacttacca
     2581 ttcgcaaatc atatggaatg cgcaatccta gatcacttat ggtttcaagt acggcaactg
     2641 cgaggtggtt gttggcgcag aaaacagcat caataggttg ttttgccttg agccaatcct
     2701 gtaatgtttt cttcacagaa tttttgatat cgctgaacgg aacttccctg accaaatctt
     2761 cttcaaatgg aattccggct tctttcaaag caagtttata gccttcctgt cgctcactga
     2821 gtgtggatat atgagcagga cttaaggtca acaatgctac ccgcttagct ccactgtcaa
     2881 taagatgctt tacagcagat tgcgagctgc gaatgttatc cactacaact gaattgcatg
     2941 agaatttcgg atagtttcgg tcaatcagta caaaggggaa ttctatcttt ttcagatcac
     3001 gaaacacttc aacattgtct gtagtgggac taataatcaa accttcaacc tgcatcttct
     3061 ctcggaggat gcgtaccaat tgtgcttctt tttcaggatc ttcatcggaa ctgcagacca
     3121 tcagatggta atcctttttc caggctaaat cttcaatcgc tcttgaaatc cgcgcataaa
     3181 aaggattgga aatatctgca acaataagtc cgatcaaatt ggtttttcct gttctcaaac
     3241 cacgcgccgc ctgattgggc atgtagttca tttccttcac cttttttaaa acccgatcaa
     3301 ctgtatcagg actaatacca tgttctttcg ccttgttatt gagaataagg gaaaccaggg
     3361 ttttggagac gcccagactt ttggcgatat cttccaaggt gatctttttc attaggaccg
     3421 tttttcgttg cgccaatgta agtcaatctg cggccaagtc ccaaaagttt actaaactat
     3481 tttaggtgct tcattaaaat tctgtccaat tgaggttcaa ttttaattta ctggtggtaa
     3541 ttacctgctc aaattccgga aagtgggcag gtgaatgggt tgtcatttaa ctgtatttaa
     3601 cgctctattt tattctcaat aacccttctg caaagggaga taatattacc tttgctcccg
     3661 aattcatatt taaacctcct atggaagtca aggaaatcaa aacagcaaat gccgataatc
     3721 cggtgattag tcagatggga atgatggggc acgagcaagt gttgttctgt caggatgaag
     3781 aaacaggttt gcgcgctatc atcgctgttc ataataccgt attgggccct gcacttggtg
     3841 gtaccagaat gtggccatac gcaactgaaa tggaagcgct tacagatgtt ttgcgtcttt
     3901 cgagagggat gacgtataaa tctgctgttg ccggtcttaa cctcggtggc ggtaaagctg
     3961 ttattattgg tgattcacgc aaagacaaat cggaggcgct gtttcgtcgt tttggaaagt
     4021 ttgtcaatag tttaggcggt aaatacatta ctgcagaaga tgttggaatt tctcctcgtg
     4081 aaatgagctg ggtaaatatg gagactaacc atgttgccgg tttgcccggt cgttccggcg
     4141 atccttctcc ggtaactgct tacggtgtat atatgggcat taaggccgct gccaaggaag
     4201 cttttggaaa tgattcgctc aatggaaagc gtgtttctgt acagggtgtt ggtcatgttg
     4261 gtgaatacct ggtcggctac ctcgccaaag aaggagcaaa gatctttatc accgatattc
     4321 atgaggaaac attggtacgt gttgccaata aatacggagc tacagtagtg ggtactgacg
     4381 agatttacga tcttgaagtc gatgtttacg caccatgtgc actgggtgct accgtgaatg
     4441 atgaaacttt attgcgcctc aaaacaccaa tcattgccgg tgctgcaaat aatcagctca
     4501 aggatgagaa gaaacatggt gaagcagtgc ttgcaaaagg aatgatttac gctcctgatt
     4561 acgccattaa ttccggcggt atcatcaatt gctacaccga ggtgaaaaac ctgaacaacg
     4621 agtgggcaat gcagaatgct gaaaatattt acaacactat ccttagcatt ataaaagaat
     4681 ctaaggcaac caatacacca acttatcttg ttgctaaccg tatggcagaa cagcgtattg
     4741 cagcagtagg taagttaaaa cgattcatgt gatgatgctt ttattcaaat tttcctaacg
     4801 ttcttttccc aatgattaac agacgacatt tacgcattaa ggttcttcag gcattgtact
     4861 cttggtttca gagtggtgat ggtgattacg gtaagtccga gcgcgagctg atgaaaaaca
     4921 ttgataagat ctacgagttg tatctcctgt atctgttatt gttatccgac ctgcaaacca
     4981 tcgcatccaa caggattgaa gacggtaagg caaaaaaact tccgacgcgt caggacctcg
     5041 aaccgaactt aaaattcgtg gagaatccgg ttttggaact catcaccaaa aacaaaagtt
     5101 tacaagccga agtacaccgc cgtaaattaa gttggggtct ggaaatggac atgctcaaaa
     5161 agatctttca ggaaatccgg aattcggatg tttttgcaaa gtatatggat gatcccggtc
     5221 gcaatttcgt ggatgctcag gagtttgtgg tgaccatttt taaagatttt atcgccaact
     5281 ccgaagcact ccaaaactgg ctcgaagaag taaatattca ctggctcgac gatatcgatc
     5341 tggtttgtgc agcggtgatc aaaaccatta aaggatttca ggaaaaatcg gatgagttta
     5401 tccgactcct gcctttgtat aaagatcccg aagaggatga gaaatttgtg aaggatcttt
     5461 ttcgcaagac gatcatgaat tctacggaaa ctgaagcact catttcctca aaaacagata
     5521 attgggagct cgaacgaatt gcatccatgg acatgatcct gatgaaaatg gcaattaccg
     5581 aggtttctga attttccacc attcctgtta aagtaaccct caacgaattc atcgagattt
     5641 ctaagtttta cagtaccccc aagagtaatg gattcatcaa tggaattctc gataaggtat
     5701 ttgccca
//
LOCUS       DAOMYG010000185         5673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4871_length_5673_cov_9.817373, whole genome shotgun sequence.
ACCESSION   DAOMYG010000185 DAOMYG010000000
VERSION     DAOMYG010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4871_length_5673_cov_9.817373"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1080
                     /locus_tag="PK610_07215"
     CDS             94..1080
                     /locus_tag="PK610_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRJ35760.1"
                     /translation="MKKFYLIPLLLIALSASGQDFHLSQYEAAALNQNPAMTGMFKGQ
                     LRVHGHYRNQWSAIATRPFTTGIIAADMPLKKVAVGMQFANFNAGAGHYNVFSALLSL
                     AYDLKIDKNNHHHISFGVQGGAFQKSVQFDKLYFGTQYTPFNGGSFDQSINPGESFAG
                     NSVIKGDLNAGLLYYYGKEAALLNPFAGVSFFHINQPKESFFNANNPLPMRWVAHGGV
                     KANISEKIQLLGKAFYMGEKNARELTYALDIHYYLAGPDAYLLFGPTFRNKDAAVIMG
                     GLKMGSFIYRLSYDINTSSLRPATNGRGGFEMSLTYIRSKTDNNPVKTCPRL"
     gene            1083..3134
                     /locus_tag="PK610_07220"
     CDS             1083..3134
                     /locus_tag="PK610_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ35761.1"
                     /translation="MKRYLPFLLILFFAAPVFGQSKKELIQLGDAAFMNGNYGSAVYF
                     YRKVVEHIPGKNDVTQPYEIQNNGGRNKKNKSSDSTAGPSNDPKEIYVTHRLAESYRR
                     LRDYNNAEYWYSKAVTMPTQQFPDARYYYAQTLMNNGKYQEAEDQFELHVNEASNESM
                     KKLSENKIVSTAFARNPENTKADTRTYWLDTSFNKGESSFGINFFTDESVIFAAARTG
                     NTTADPKEDGRYTTDLYIAAELDSGKFGVPQPFPSPVNSPANEGACVLSVDRSTFYFT
                     RWSPSNKNDCAIYVSKRFNRRWMEPMKLDINLEGYRTMMPMLNLDETKLFFASDRPGG
                     QGGLDIWFCPIDPEGNIGTPENLGSVINTPEDESSPYFHLITNTLYFASEGHRGFGGL
                     DIFKTVYDEEESIWKNPINLGAPINSSKDERYFILSNDQRSGYFASDRNPCNDCDSLA
                     MMNPHCEKIYGFQKESIIIDIEGYVYDAETEEPIMGAIVELKDVNGEWEPIILITDEN
                     GYYKTELRQGMEIFMKAKKTKFFADAASVNTVSITETTHLQQDFYLRRIPSGEIEIPG
                     IEYDFDRATLRPKSKEILDQLYDFLTLNSDLVVEIKSHTDCRGNDLYNLRLSKERAQS
                     CVDYLISKGIAKERLIPQGYGETEPLFKCDDIEKETVKDKQEEMHQRNRRTAFRVVKQ
                     E"
     gene            3336..>5673
                     /locus_tag="PK610_07225"
     CDS             3336..>5673
                     /locus_tag="PK610_07225"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35762.1"
                     /translation="MIKSLSIAIAVFVLSTPWAKAQIDTDFWFAAPEVSASLGDQPIT
                     LRLVSYSQAATVTISQPANGAFVPIVVNLPANAQNAVNLTPFLASVESPAANVVSNNG
                     IRIQSTQPIGAAYEIGAASNKEIISLKGAKGLGTEFYTPFQRFWNSGSTTPASTSSFE
                     IVASDNATTVLITPRAAMVGRAANVTFSITLNAGQTYSGREVDLLASTSLSGSIISSN
                     KPISVTVHSGAMSSGACMSTVADQITSTSHLGRNHIIHKTYATNERVYVMAVQNGTSI
                     TVTNSGTTSTLINWGETFEFPLSDTINYIQSDKPVYVFHITGNGCRLSGAQVPKLLCA
                     GTYSTAFARPVTDSFALVLYTRTGFENQFQLNGNASLIPASAFRTVPGTNGDYKSALI
                     WYSTANIPANTHQVVTNSGDIFGLGIRYGSTTAGSAYAFLSEFSSYPFVEAGTSTTVC
                     ANVPLNLNGIVGGGSVTGTWSSTGFGSFQNGLTNLSNTYIPSPLDTVISPIRIILSST
                     GPCPVQRDTLTLIVEPAPIVSASADQTVCSNNAQVQLNGSVTGGATTGIWSTIGTGSF
                     SPSATALNAIYTPGAAQISAGTATLVLTATNIGTCASVSDTMIVSITNGATADAGPAT
                     LSVCANNPVISLSGTIGGSSTTGRWTTTGNGVFSPSNLSLVTTYTPSVTEVNSGSVKL
                     YLESTNNGTCFPAIDSIMVTFTPRPTVSAGTNLLACTNDAGVTLNGSIGGPTTTGIWT
                     GGAGTFSPSNTALNASYQPTTTEISNGNIILTLTSTNNG"
BASE COUNT         1697 a         1276 c         1185 g         1515 t
ORIGIN      
        1 gtataccaac tccgggctgt tactgaagat ggtgtagaac atattttaac cggagacgtg
       61 acacttttgc gttaatcttt tatcgaagaa aaaatgaaga aattctacct cataccactt
      121 ctgctcattg cactatctgc aagcgggcag gattttcacc tttcgcaata cgaagcggct
      181 gcattgaatc aaaatcctgc aatgaccgga atgttcaagg gacaactgcg agtacatggt
      241 cattaccgca accaatggtc tgctattgca acccgcccat tcacaaccgg gattattgca
      301 gctgatatgc ccttaaaaaa agtcgctgtt ggaatgcagt ttgccaactt taacgcagga
      361 gccggtcact ataatgtttt ttctgcgctg ttgtcgctgg cttacgatct taaaattgac
      421 aagaacaatc atcatcacat cagttttggg gtgcagggtg gagcatttca aaaaagtgtt
      481 cagtttgaca agctttattt tggaacgcaa tacactccgt ttaatggcgg aagttttgat
      541 cagagcataa acccaggcga aagttttgca ggaaacagcg ttatcaaggg cgatcttaat
      601 gcaggattgc tttattacta tggtaaagaa gctgcattac tcaatccttt tgcaggagtc
      661 agttttttcc acatcaatca accaaaagaa tcatttttca acgctaacaa tccattgccc
      721 atgcgttggg ttgcacacgg aggagtaaag gcaaacatta gtgaaaaaat acaactgttg
      781 ggtaaggcat tttacatggg agaaaagaac gcccgtgaat taacctatgc actcgatata
      841 cattactatt tagccggtcc cgatgcgtat cttttatttg gaccaacatt cagaaataaa
      901 gacgcagcag taattatggg aggcctcaaa atgggaagct tcatttacag attaagttac
      961 gacatcaata cctcgtcact ccggccggca actaatggcc gaggtggttt tgaaatgtcg
     1021 ctgacgtata tccgttcaaa aaccgataac aatccagtta aaacctgtcc acgactctga
     1081 gcatgaaacg atatcttccc tttttgctga tactcttttt cgctgctccg gtttttggtc
     1141 agagcaagaa agaactcatc cagttgggtg acgcagcatt tatgaatggc aattacggat
     1201 ctgctgttta tttctacaga aaagttgtag aacatatacc tggcaagaac gatgttaccc
     1261 aaccttacga aattcagaat aatggtggcc ggaacaagaa aaacaaaagc agcgacagca
     1321 cagctggtcc aagcaatgat ccaaaggaaa tttatgtaac tcaccgactg gcagaatctt
     1381 atcgcagact aagagattac aacaatgccg aatattggta cagtaaagcg gtaaccatgc
     1441 caacgcaaca gtttcctgat gcaaggtatt attacgctca aacattaatg aataatggta
     1501 aataccagga agcagaagat cagtttgaat tgcatgtaaa tgaggcaagc aatgaaagca
     1561 tgaaaaagct ttcggaaaat aaaattgtta gtaccgcctt tgcacgaaat ccggaaaaca
     1621 ccaaagcaga tacacgtaca tactggctgg atacgtcttt taacaaaggt gaatcctcat
     1681 ttggaattaa ttttttcact gatgaatccg taatttttgc agcagccaga acaggaaata
     1741 ctactgcaga tccaaaagaa gatggccgct atacaaccga tctgtacatc gctgccgaac
     1801 tggacagtgg aaagtttggc gttccccagc cttttccttc tcctgtaaat tctcctgcca
     1861 acgaaggcgc ctgtgtattg tctgttgacc gaagcacttt ttattttaca cgttggtcgc
     1921 cctccaataa aaacgattgc gctatttacg taagcaaacg tttcaacaga agatggatgg
     1981 aaccaatgaa actcgacatc aatcttgaag gctatcgaac aatgatgccg atgttaaatc
     2041 tggatgaaac caaattgttt tttgcatctg atcgtccggg cggacaaggc ggattggaca
     2101 tctggttttg ccccatcgat cccgaaggca acattggcac accggaaaat cttggttcgg
     2161 taatcaacac ccctgaggac gaatcatctc cttattttca cttgatcacc aacacgcttt
     2221 attttgcttc tgaaggtcat cggggatttg gcggtttaga tatattcaag acggtttatg
     2281 atgaggagga aagcatctgg aagaatccga ttaatctggg agcaccgatc aattcatcaa
     2341 aagacgagcg ttatttcata ctaagtaatg atcagcgaag cggttatttt gcatccgacc
     2401 gtaatccatg taacgattgc gattcgcttg caatgatgaa tccgcattgt gaaaagattt
     2461 atggttttca gaaagaatcg atcatcattg atattgaagg atacgtatac gatgcagaaa
     2521 ccgaagaacc gattatgggt gctattgtgg agttaaagga cgtaaacgga gaatgggagc
     2581 ctattattct cattacggat gagaacgggt attacaaaac cgaacttcgc cagggcatgg
     2641 aaatattcat gaaagccaag aaaacaaagt tctttgcaga tgctgcgagt gtaaatacgg
     2701 taagcattac tgaaacaaca catcttcagc aggacttcta cctccgtcgt atcccaagcg
     2761 gtgagattga aattccggga attgagtacg atttcgacag agccactctc cgaccgaaat
     2821 ccaaagaaat tctggatcag ctgtacgact tccttaccct taacagtgat ctggtggtcg
     2881 agatcaaatc gcacaccgac tgccgtggaa acgatctcta caacctccgt ttatccaaag
     2941 aaagagcaca atcctgcgta gattacctga tctcaaaagg tattgccaaa gaacgtctaa
     3001 tcccccaggg gtatggggaa acagaacctc ttttcaagtg cgatgacatt gaaaaagaaa
     3061 cggttaagga caaacaggaa gaaatgcatc agcgaaacag aagaacggca ttcagggttg
     3121 taaaacagga gtaagacgca acattctgtc actttttacc gtctgtttat caccgaccaa
     3181 aatccgtcta tcaggtgatt aaccctttat tccattcgac cgacgcccat attttgagga
     3241 tttgtgggca tttatcccta attttgtgcc ccgaatgagg aaatctcaga aaacacaccc
     3301 attctgttgc tattgaataa attctacttt gaacgatgat aaaatcgctc tctattgcta
     3361 ttgcggtatt cgtattgagt accccttggg ctaaggccca gattgatacc gatttttggt
     3421 ttgcagctcc tgaagtttct gcttcattgg gagatcaacc cataacacta aggttggtga
     3481 gttattccca ggccgctact gtaacgatca gtcagccggc caatggagct ttcgttccta
     3541 ttgtcgtcaa tcttccggct aacgcacaaa atgcagtcaa tctaactccc ttcttggcca
     3601 gtgtggagag tcctgctgca aacgttgttt ccaataacgg aatacggatt caatctactc
     3661 aacctattgg agctgcctat gaaattggtg ctgcatccaa taaggaaatt atttcactga
     3721 aaggtgctaa aggattggga actgaatttt atactccttt tcagcgtttt tggaattcag
     3781 gttctacaac tccggcatcg acttcatcct ttgaaattgt tgcaagtgat aatgctacaa
     3841 ccgtattaat tactccaaga gctgcaatgg tgggaagagc tgcaaatgtt actttcagca
     3901 tcacactaaa tgccggacaa acatacagcg gaagagaagt tgatttactt gcttcaactt
     3961 ccttatcggg ctccattatt tcttctaaca aacccatttc tgtaaccgta cactccggag
     4021 cgatgagcag tggtgcttgt atgtcgactg ttgcggatca gattacttct accagtcatc
     4081 tcggaagaaa ccacatcatt cataaaacct acgccacaaa tgaacgcgtt tatgtaatgg
     4141 cagtacaaaa cgggaccagc attacagtaa ccaatagtgg aacgacttct actcttatca
     4201 actggggaga aacatttgaa tttccgcttt ccgatacgat taattacatt cagtcggata
     4261 aaccggttta tgtttttcac atcaccggaa acggatgccg cctctccggt gcacaggtgc
     4321 ctaaattgct ttgtgcagga acttattcaa ctgcatttgc acgtccggta accgattctt
     4381 ttgcgcttgt actttataca cgtaccggtt tcgaaaatca attccagctt aatggtaatg
     4441 catcgctgat tcctgcaagt gcgttccgga cagttccagg caccaatggt gattataaaa
     4501 gcgcactcat ttggtatagc accgccaaca ttcctgccaa cacccatcag gttgttacta
     4561 attcaggaga tattttcgga ttgggaattc gttatggaag tacaacagca ggttctgctt
     4621 atgcatttct ttcggagttc agttcttatc cattcgttga agccggaacc agtacaacag
     4681 tttgtgcaaa tgttccactt aacctaaacg gtattgttgg cggaggttca gtaacaggaa
     4741 cctggagcag tacaggattc ggttcatttc agaatgggtt gaccaaccta agcaacacgt
     4801 atattcctag tccgctggac acagtaatca gtccgattcg aattattcta tcgtcaacag
     4861 gcccctgtcc tgtacagcgc gacactttaa ctcttattgt tgaacctgct ccaattgtaa
     4921 gcgcatctgc agaccaaaca gtttgcagta ataacgcaca agtgcaatta aacggatcag
     4981 taacaggtgg agcaacaacc ggaatctgga gcacaattgg taccggatca ttctcgcctt
     5041 cagcaaccgc actaaacgcc atttacaccc cgggagcagc acaaatttct gcaggaacag
     5101 caacacttgt cctcactgca accaatatcg gaacatgtgc ctccgttagc gacacaatga
     5161 tcgttagcat taccaatgga gcaactgcag atgccggtcc tgccacatta tcggtatgtg
     5221 cgaataaccc agtaatatca ctcagtggta ctatcggtgg aagttccaca acaggacgct
     5281 ggactacaac aggaaacggt gttttttcac cttccaatct ttctttggtt acaacctata
     5341 ctccaagtgt tacagaagta aatagtggaa gcgtgaaact gtatttggaa tcgaccaaca
     5401 acggaacatg tttccctgca attgacagta taatggtaac ctttactccg cgtccaactg
     5461 tttctgccgg caccaattta cttgcctgta ccaacgacgc cggtgttacc ttaaacggaa
     5521 gcattggcgg acccacaact accggaatct ggacaggcgg agcaggtaca ttttcgcctt
     5581 ccaatactgc gcttaacgca agctaccaac caacaaccac ggaaatttca aatggaaaca
     5641 ttattctaac gcttacaagt accaacaacg gaa
//
LOCUS       DAOMYG010000186         5658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4889_length_5658_cov_7.922363, whole genome shotgun sequence.
ACCESSION   DAOMYG010000186 DAOMYG010000000
VERSION     DAOMYG010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4889_length_5658_cov_7.922363"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1239
                     /locus_tag="PK610_07230"
     CDS             67..1239
                     /locus_tag="PK610_07230"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HRJ35763.1"
                     /translation="MIYTIEWLTKVLLCQGVILLAYLFIKSEPLFSLRRVWMIGALLV
                     PFLLPFVEGIFLTNESDGHFFFYEFEPVTVYAGSKLESISWTAIMFWSVAIVIGLKLA
                     GFLRTVFFVLFRTKKIAHGLRVWNGSFSFSFFRIIILAEKDQNDEMVLAHESAHVQMH
                     HYFDLIVSEVVTGLFWFSPFVWVFNRELKKIHEFQADQYVLKVFGVIEYKECLFEKAT
                     GFRPLTGSHSFGSLTLKNRFMMMTKKSSALAGTRIAFATIMLFSWVGMVGFAAPEQPL
                     GSKSESQVPIVDLENQPDVYPVFPGGQDAMIKYLSGNINYPESARIAGKEGKVIVKFV
                     VDKTGKIKDVKIASGVEASLDAEAMRVVKEMPDWTPGEKGGKKVNVEMVLPIQFKL"
     gene            1412..4168
                     /locus_tag="PK610_07235"
     CDS             1412..4168
                     /locus_tag="PK610_07235"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRJ35764.1"
                     /translation="MDNYSYLSNAHPDAIEEMYSRYLSNPDSLEHGWKKFFEGFEFAR
                     TNFEDSGVLPENVTAEIKVMNLIHAYRQRGHLFTKTNPVRDRRKYTPTLDIENFGLAQ
                     SDLEKVFQAGAQIGIGAAKLKDIVAHLDQTYCQSIGVEYLYMRQPEVIDWLREKMEGS
                     KNTPSFSVDEKKTILNKLNQAVVFENFLATKFVGQKRFSLEGGESLISAMDMLMETGA
                     DLGIKDYVVGMAHRGRLNVLANILNKTYRDIFTEFEGRLSEDSLFDGDVKYHLGYSSD
                     QTSNNGKKVHISLTPNPSHLETVDAIVEGITRAKMDNLHNGDENSVCPVLIHGDAAVA
                     GQGIVYEVIQMSQLDGYRVGGTVHLVVNNQVGFTTNYLDARSSTYCTDVAKVTLSPVF
                     HVNGDDVEAVVFVAKLAMEFRQKFHRDVFIDILCYRKYGHNEGDEPRFTQPVLYKAIQ
                     RHPNPREIYVKKLVEQGSIEAGMAKDMERSFKDLLEGMLNEAKQTEKAKITSFLEGQW
                     AGVKMAAPDAFDKSPDTAIDKKVFLDIAGKIVSLPDGMNFFNKIQKLFEDRKTMIAND
                     QYDWAMGELMAYATLMHEGHHVRFSGQDVERGTFSHRHAVVKVEDSEEEYTPLNNFGA
                     KGQLRIFNSHLSEYGVLGFEYGYAMAAPNALTIWEAQFGDFFNGAQIIIDQYISSAEY
                     KWRRMNGLVVLLPHGYEGQGPEHSSARMERFLQMCAENNMQIVNATTPANQFHVLRRQ
                     IKREFRKPLICFTPKKLLRFPACVSSVKDFTSGGFQEVIDDVSVKAADVKKLVLCSGK
                     IYYEMMERKAQLGSKDVAFVRIEQLYPWPAKQIAAILSKYSNAKEICWLQEEAENMGA
                     YTFVMKKMKDVDLTYIGRKESASPATGYAKLHNAQSEEIFAKVFGAVNPVVESKSKKK
                     ESVK"
     gene            4199..5443
                     /gene="odhB"
                     /locus_tag="PK610_07240"
     CDS             4199..5443
                     /gene="odhB"
                     /locus_tag="PK610_07240"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HRJ35765.1"
                     /translation="MAIIEHKVPSPGESITEVVIARWIKSDGEFVDKDDELCEIDSDK
                     ATLTVNAEESGQLKIMAAEGDTVKVGQIICSVDSSVKGEAKPKTDAPKAETKKEEKKS
                     EPVPNPMPVAQEKSYATGHPSPAAKKLMDEKGISSSQVQGSGPGGRVTKQDVQNALTL
                     GFSYDKPAFGGTREQERKKMTTLRKKIAERLVSVKNETAMLTTFNEVDMKPIMDLRKK
                     YKDKFEKTHGIGVGFMSFFTKACTVALQQFPAVNAYIDGEEMVFNNYCDVGIAVSTPK
                     GLMVPVLRNAETLSLAGIENGIKEMAIKARDGKLSIDDMTGGTFTITNGGVFGSMLST
                     PIINPPQSAILGMHNIVERPVAIDGRVEIRPIMYVALSYDHRIIDGKESVSFLVKVKE
                     MLENPSRMLFDGRDPLEVVLGI"
BASE COUNT         1755 a          955 c         1317 g         1631 t
ORIGIN      
        1 tgaaggatat ccgtaaaggc aagaagtcgg aaaagaaaaa gaagaaaaag aagaaataat
       61 ccgagtatga tttatacaat cgaatggctg acaaaggtgt tgctttgtca gggtgttatt
      121 ttattggctt atttattcat taaatcagaa cctctttttt cattgaggag agtttggatg
      181 ataggtgcat tattggtacc ctttttactt ccgtttgtag agggaatttt cctgacaaat
      241 gaatcggatg ggcatttttt cttttatgaa tttgaacctg taactgttta tgccggaagt
      301 aaattggaaa gtatatcctg gactgctatt atgttctgga gtgtcgcgat tgtgattgga
      361 ttaaaactag caggtttttt aaggacggtt ttctttgttt tatttcgaac taagaaaatt
      421 gcacatggat taagagtttg gaatgggtca tttagttttt ccttttttcg aataataata
      481 ttggcagaaa aggatcaaaa cgatgaaatg gttcttgcgc atgaatcggc ccatgttcaa
      541 atgcaccatt attttgattt gattgttagt gaagtagtaa caggattatt ttggtttagt
      601 ccgtttgtgt gggtttttaa tcgcgaatta aaaaaaatcc atgagttcca ggcagatcaa
      661 tatgtattaa aggtatttgg agttatagaa tataaagaat gtttatttga aaaagcaaca
      721 ggattccgac cattaacagg gtcgcattca tttggaagtt taaccttaaa aaatcgtttt
      781 atgatgatga caaaaaaaag ttccgctttg gccgggacaa gaattgcgtt tgcaactatc
      841 atgctttttt catgggttgg tatggtagga tttgctgctc cggagcaacc actgggttcg
      901 aaatcagaat ctcaggttcc gatagttgat ttggagaatc aacccgatgt ttatcctgtt
      961 tttcccggag gtcaggatgc aatgattaaa tatctttcag gaaacatcaa ctacccggaa
     1021 tctgcaagaa ttgccggaaa ggaaggaaaa gtgattgtta agtttgtagt ggataagaca
     1081 ggaaagataa aagatgtaaa aattgcatca ggcgtcgaag caagtttgga tgctgaagct
     1141 atgcgcgttg tgaaagaaat gcctgactgg actcctggtg aaaagggagg aaaaaaggtg
     1201 aatgttgaga tggtattgcc aattcagttc aagctttgat ttcaacctga aatttgaccg
     1261 ggcgattcat tccggattaa taagcaggaa tgaatcgccc gtttgtttat taatttgttg
     1321 ttatacaggt tattgagtcc ggtcctgaag attatcttcc tgtgttttta tataggggaa
     1381 aagcgtaaat ttgtcgtcca aacacagtga aatggacaac tattcgtacc tgagcaatgc
     1441 acacccggat gctattgagg agatgtattc acgttacctt tccaatccag attctttgga
     1501 acacggatgg aaaaagtttt ttgaaggttt tgagtttgcg cgtacaaatt ttgaagacag
     1561 tggtgtatta cctgagaacg taacggcaga gatcaaggtc atgaacctga ttcatgctta
     1621 tcggcagcgt ggacatttgt ttacaaaaac taacccggtt cgcgatagaa gaaagtatac
     1681 acctacgcta gatattgaga attttggttt ggcccaatcg gatcttgaaa aagtatttca
     1741 agccggtgct cagattggaa tcggagcagc gaagttgaaa gatattgttg cgcatttgga
     1801 tcaaacctat tgccagagta taggcgttga gtatttatat atgcgccaac cggaagtaat
     1861 agattggttg cgcgaaaaaa tggagggaag taaaaacaca ccttcatttt ctgttgatga
     1921 aaagaagaca attcttaaca agctaaatca ggcagtagtt tttgaaaatt ttcttgctac
     1981 taaatttgtt ggtcagaaac gattttcact cgaaggtgga gaatctctaa tctccgcaat
     2041 ggatatgttg atggaaaccg gagcggatct tggtattaaa gattacgtgg taggaatggc
     2101 gcatcgtggc aggttaaatg ttctcgcaaa tattctgaat aaaacatacc gcgatatttt
     2161 cacagaattt gaagggcgtt tatcagagga ttcacttttt gacggagatg taaaatatca
     2221 cctgggttat tcaagtgacc aaacttccaa taatggaaaa aaggtacata tttcacttac
     2281 tcccaatcca tcgcacctgg aaacagttga tgccattgta gaggggatca cacgtgcaaa
     2341 aatggataat cttcataatg gagatgaaaa ttcagtttgt ccggtcttga ttcatggtga
     2401 cgcagctgtt gctggtcagg gaatcgtgta tgaggtaatt cagatgtcgc aattggacgg
     2461 ataccgcgta ggcggtacag tacaccttgt agtcaataat caggttggat ttacgacaaa
     2521 ctatctcgat gctcgttcat caacttattg tacggatgtt gcaaaagtta ctttatcgcc
     2581 tgtttttcac gtaaacggtg acgatgttga ggcagtggtt tttgttgcaa aactggcaat
     2641 ggaattccgt cagaaatttc atcgcgatgt tttcattgat attctctgtt atagaaagta
     2701 cggacataat gaaggggatg agccacgctt tacccagcca gttctttata aagccattca
     2761 gcgccatccg aatccacgtg agatttacgt gaaaaaactt gttgaacaag gttcgatcga
     2821 agccggtatg gcgaaagaca tggagcgaag ctttaaagat ctgctggagg gaatgctcaa
     2881 tgaagcaaaa caaactgaaa aggcgaaaat cacctcgttt ctcgaaggac aatgggcggg
     2941 tgttaaaatg gctgcacccg atgcgtttga taaatcacca gatacagcta ttgacaaaaa
     3001 agtatttctt gatatagccg gtaaaatagt ttcattaccc gatggtatga atttcttcaa
     3061 taagatccag aaactttttg aagacagaaa aaccatgatt gccaatgatc agtacgattg
     3121 ggcaatgggt gaactaatgg cctatgcaac tttaatgcac gaagggcatc atgttcgctt
     3181 ctctggtcag gatgtagagc gcggcacatt ttcacatcgt cacgctgtgg taaaagttga
     3241 agattcagaa gaagaatata ctcctttgaa caattttggt gcgaaaggcc aattgcgcat
     3301 atttaattcc catttgagcg aatatggcgt gcttggtttt gagtatggat atgcaatggc
     3361 tgctccgaac gcactgacaa tatgggaagc gcaatttgga gatttcttta acggagctca
     3421 aattattatt gatcaatata tttcatcggc cgaatacaag tggagaagga tgaatggtct
     3481 tgtggttttg ctaccgcatg gttacgaagg acaaggacct gaacactctt ctgcacggat
     3541 ggaacgattc ctgcaaatgt gtgccgagaa caatatgcaa attgtaaatg caactactcc
     3601 tgcaaatcag ttccatgttt tacgccgaca aataaaacgc gaatttcgta aaccattaat
     3661 ttgttttaca cctaaaaaac tgcttcgttt tccggcttgc gtttcttcag tgaaggattt
     3721 taccagcggt ggatttcagg aggtaattga tgatgtttcc gttaaagctg ctgatgtaaa
     3781 gaaactcgta ttgtgttcag gtaaaatcta ttacgaaatg atggaacgca aagcacaatt
     3841 aggcagtaaa gatgttgcgt ttgttcgaat cgagcagctt tatccatggc ctgcaaaaca
     3901 aattgcggct attctttcca aatattccaa tgccaaggag atttgctggt tgcaggaaga
     3961 ggctgagaat atgggcgctt atacttttgt aatgaaaaaa atgaaagatg tagatcttac
     4021 atacatcggt agaaaagaaa gcgcgagccc ggctacaggt tatgctaaac tccataatgc
     4081 acaaagcgaa gagatttttg ccaaggtatt cggagcagta aatccggttg tggaaagcaa
     4141 atccaaaaag aaagaatcag taaaataatt ttttaatcaa gtacatacac cctaaccaat
     4201 ggcaatcatt gaacataaag taccgagtcc cggcgaatca atcactgaag tagtaattgc
     4261 acgctggata aaatcagacg gcgaattcgt tgataaagat gatgagcttt gcgaaattga
     4321 ctccgataaa gcaacgctaa ccgtaaatgc tgaagaatcc ggtcagctta aaattatggc
     4381 cgccgaaggt gatactgtca aagttggaca gatcatttgt tcagttgata gttctgtgaa
     4441 aggtgaagct aaaccaaaaa cagatgcacc aaaagcagaa actaaaaagg aggaaaagaa
     4501 atcggaaccg gttccgaatc ctatgccggt tgcgcaggag aaatcctatg caacaggtca
     4561 cccttcgcct gcagcaaaaa aattaatgga tgaaaaaggt atttcttctt cacaggttca
     4621 aggatcaggt cctggaggaa gagtaacaaa gcaagatgtg caaaatgcgc ttacacttgg
     4681 tttctcttat gacaaacctg catttggtgg cactcgggag caggagcgta aaaaaatgac
     4741 aacgcttcga aaaaagattg ctgagcggtt ggtgagtgtg aagaatgaaa cagctatgct
     4801 gaccactttt aatgaggtgg atatgaaacc gataatggat cttcgcaaga agtacaaaga
     4861 caaatttgaa aagacgcatg gtatcggtgt aggattcatg tcgtttttca caaaggcttg
     4921 tactgttgca ttgcaacaat tccccgcggt aaatgcatat atcgacggtg aagaaatggt
     4981 gttcaacaac tattgcgatg taggtatcgc tgtaagtaca ccaaagggat tgatggttcc
     5041 tgtattgcga aatgcagaga cattgagtct tgccggtatt gaaaatggca ttaaggaaat
     5101 ggcaattaaa gcgcgcgatg gaaaattatc tattgatgat atgaccggtg ggacttttac
     5161 cattaccaat ggaggagtgt ttggttcaat gctttcaact ccaattatta atcctcctca
     5221 atctgcaatt ttgggaatgc acaacattgt tgagcgtcct gttgcaattg atggccgtgt
     5281 tgaaattcgt ccgattatgt atgtggcgct ttcttacgat caccgtatca ttgacggtaa
     5341 ggaatcggtt tctttcctgg tgaaagtaaa agagatgttg gagaatccat cacgcatgtt
     5401 gtttgatggc cgcgacccgt tggaagtggt tttaggaata taagtataaa aaaagagcct
     5461 caatcgaggc tctttttttt tggcttatgt agtttttccc cttaatgcat cggtaagatt
     5521 tgaagcgctt acattgatgc tttttcttct ggaaaccaga taaattaagg cagctggcca
     5581 aaaaacaaaa accaaaatca caaacacagt cggtcatcgt gcgagtgtcg gcagatggtc
     5641 agattcgtct cctagagc
//
LOCUS       DAOMYG010000187         5638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4919_length_5638_cov_9.605230, whole genome shotgun sequence.
ACCESSION   DAOMYG010000187 DAOMYG010000000
VERSION     DAOMYG010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4919_length_5638_cov_9.605230"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1892)
                     /locus_tag="PK610_07245"
     CDS             complement(<1..1892)
                     /locus_tag="PK610_07245"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35766.1"
                     /translation="MEVKLNASDGAAADEFGSSVAVYDSLLISGSPRNNASVTDCGSA
                     YIFRHNGTSWIEEQMLTALDAAIGDRFGYSVDIKGNVAIVGAPFDDDLGTNSGSAYIF
                     RFNGFAWIQEYKLIALDGNAGDEYGYSVSINDTVAVVGAYRSNNGNPGNGAVYVYRYN
                     GISWNQMQKLTAPAAQIDDFFGYSVDLHKDRIIAGCYQDDDMGVNSGSAFVFHYNGTT
                     WVQEQKLLPANGTAGDHFGFSVSINDTLALIGAYAKNTAATASGAAYVFTRTGSTWTQ
                     QQQLTASDGLTDDWFGFSVSISGRTLTCGAFHNDDLGIESGSAYLFRYTGSTWIPEMN
                     LRASDGAAQDNFGYCVSVSGNTIAIGSPLNDDNGSASGSVYTYDLCKHIPIQELCMVS
                     VDNNFNDILLLWEKPTTTLTSTYNIYRNSGSGPQLIYNQPYNLFSEHLDNTVNVDAQS
                     YSYHLTTTNHCGVESAAGTVHSTMHLTTTPGTPGQVTLNWTPQTGFVFPFYRIWRDTA
                     VTTNYVLIFATLNTQFAYTDNALVVGSPANYYMEVQRTAGSCNSGNTSRSAAYSNASD
                     PFSVSVNEIGTTPLELYPNPANEYIQFQIPDYNGEALTVDLFDNGGRRGTPPDERQHQ
                     PHSCRAP"
     gene            2037..2561
                     /locus_tag="PK610_07250"
     CDS             2037..2561
                     /locus_tag="PK610_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35767.1"
                     /translation="MQSIRKFFRLLEYASITGILIMALLFFTGHLSFSWLKFFVALFD
                     VLVVLFIFLNCTDDYKTPFEKLFRLIGWLVLLTGIVLIFVIYESVRPDAYWHWFNTIL
                     VTGLFAAQLSEINELKRSNDVLKLLNLVVTLGAYTVLIMTIHGFPMNFVLLFILLGVN
                     MLTMLATVLTGKLP"
     gene            complement(2562..4112)
                     /locus_tag="PK610_07255"
     CDS             complement(2562..4112)
                     /locus_tag="PK610_07255"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HRJ35768.1"
                     /translation="MQRNVNSCWILLFRLCIVLIPATVFAGNDTTLVLKKTIRGGLTP
                     KSIVHNGNGLFFAQNMMYSHTISVFDENYRLVKTISDRIQPDRFGIKDREGSFRGAPV
                     ECAFTPDGKYAYVSNYCMQGDGFEHPGCDTCYGKEYDISYIYKINTESLEIENIIPAG
                     SVPKYLAISPDGKKMLVSNWSSGDVTLFDLSTEKELKRFHVGRFPRGIAIDSKSRYAY
                     VTVMGGTSIQRIDLEELSSSKFMEVGKGPRHLCIDSRDSLLYVSVNNEGNIARINLHT
                     GNMQKLFVGSAPRSMVLGKGDRFLYVVNYSASTFSKIDLEQFSVAEKAATGKNPIGIT
                     FNERTNEIWVACYSGSVMVYKDLALPKGNLPLAGYFGMQPVFDDLYEGWNLLFSKKEI
                     ASHPIQGSSGKTPASSKAGNGVNQNEHFTEKSIANTLPNEKPAVATTTETGTFYVVTG
                     SFKNSENAGRKTKELIQSGFKGKMVDSGKGLQYAAIGGFESKDKAQTELDRYKSQTGL
                     DAWVWEKK"
     gene            4192..>5638
                     /locus_tag="PK610_07260"
     CDS             4192..>5638
                     /locus_tag="PK610_07260"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRJ35769.1"
                     /translation="MKKQLFLLLALIASQFSYAEEGMWIPSLLKKLNANELKAAGLKI
                     PVEKIWSTKESSIKDAIVHFGGGCTAEVLSYEGLILTNHHCGYSQIQAHSSLENDYLK
                     NGFWAMNRTQELPNPGLTVSFVVDMVDVSDQVMRGCIDENGRVNNEMIKANIERVRQA
                     NAKDGYEIAVRPFNYGNSFFMIITETYRDVRLVGAPPSSIGKFGGDTDNWVWPRHTGD
                     FTLFRIYAGKDNRPADYSPDNVPYRPKYSLPVSMMGVKENDFTMVFGFPGTTQQFLPA
                     AAVEFLVKYRNPARIKMRETALNIIDAEMRSSDLIRIQYSAKQARISNYYKKWIGENN
                     GLIRLNAIDKKKELEREFIDRAKRDRNDLGRFGQLPDRFVDLYIAWQPFGTAYDYFSE
                     LVYSGPEFLRYAQGFEKLVEAYSVKSVGNEGKSLLDRISAAVPGYFKNYNAATDKKLF
                     VALFPMYLDGTPEALQPDGIPAMKKKYKGDWN"
BASE COUNT         1531 a         1165 c         1243 g         1699 t
ORIGIN      
        1 ggggcgcggc atgagtgcgg ctgatgttga cgctcatctg gcggagtgcc gcgccttcct
       61 ccattgtcaa aaaggtcaac tgtcagtgct tctccgttgt aatcggggat ctggaattga
      121 atgtattcat ttgccggatt ggggtaaagt tccaatggag ttgtaccgat ttcgtttact
      181 gaaactgaaa agggatccga tgcattggaa taagctgcag atcggctcgt attgccggaa
      241 ttgcaagaac ctgcagttcg ctgtacttcc atataataat tggcaggaga acccactaca
      301 agtgcgttat ctgtataggc gaattgtgta ttaagcgtag caaaaatcaa aacgtaattg
      361 gtggtcactg cagtgtcgcg ccatattcgg taaaacggaa atacaaatcc tgtttgtggg
      421 gtccagttta aggtaacctg acccggcgtt ccgggagtgg tggttaagtg cattgtagag
      481 tgaactgtac ctgccgcact ttcaacaccg caatgattgg ttgttgttag gtggtaggaa
      541 tatgattgtg catctacatt aaccgtattg tctaaatgct cactgaagag attataaggc
      601 tggttgtaga tgagttgcgg tccggaacca ctgttgcgat aaatgttata cgttgaggta
      661 agagtggtcg ttggtttttc ccaaagcaat aaaatgtcgt tgaaattatt gtcgactgaa
      721 accatacaca gctcctgaat aggaatatgc ttgcaaagat cataggtgta aactgatccg
      781 cttgcagaac cattgtcgtc attaagtggt gaaccaattg ctattgtatt ccccgaaaca
      841 gaaacacaat atccaaaatt gtcttgcgct gcaccatcgc tggcacgcag attcatttca
      901 ggtatccagg tgcttcccgt gtatctgaat aaataagcag atccgctttc gatccctaaa
      961 tcatcattat gaaatgcacc gcaagtaagt gtcctgcctg aaatcgaaac tgaaaaacca
     1021 aaccaatcat cagtcagccc atcggatgcg gttaattgct gttgttgggt ccatgtgctt
     1081 cctgttctgg taaatacata ggctgctccc gaggccgttg cagcagtgtt ttttgcgtat
     1141 gctccaataa gtgccagtgt gtcgttaatg ctaaccgaaa atccaaaatg atcgcctgca
     1201 gtaccgttgg cgggaagtag cttttgttcc tgtacccagg tcgtgccatt gtaatgaaat
     1261 acaaaagctg aaccggagtt tactcccata tcatcatcct gataacatcc tgcaataatt
     1321 ctgtctttgt ggaggtcgac actataccca aagaaatcat caatctgcgc agctggagct
     1381 gttaatttct gcatttgatt ccagctgatc ccattatagc ggtaaacata aacagctccg
     1441 ttacccggat tgccattgtt cgaacgatat gcgccaacaa cagccacagt atcgttaatg
     1501 ctaacactat atccgtattc atcacctgca ttcccgtcaa gcgctatcaa tttgtattcc
     1561 tgaatccatg caaaaccatt gaaacgaaat atgtaggcag agcctgagtt ggttccaaga
     1621 tcatcatcaa acggcgctcc gacaatggcg acattcccct taatatcaac cgagtatcca
     1681 aatcgatcgc caatggcggc atccagtgcg gtaagcattt gctcttcgat ccaggatgtt
     1741 ccgttatggc gaaaaatata ggcggagcca cagtctgtta cagaggcatt atttctgggt
     1801 gatccggata taagtagact gtcatagaca gctaccgaac taccgaattc atcggcagcg
     1861 gcaccgtcag atgcgttgag tttgacctcc atttgtgcga ataacaagga ggaccaaaat
     1921 atggctatgc aagcagcagt taatttcatt agtgctaatt acggagtaat acaggaaaaa
     1981 gttgggtgat tgttgataaa gtggttggat gttgaaaaac tttgtagttt taccccatgc
     2041 agtcgatccg taaatttttc cgcttgcttg agtatgcgtc cattacaggg attttgataa
     2101 tggcgctgtt atttttcaca ggtcatctca gcttttcctg gctcaaattt tttgtcgctc
     2161 tttttgatgt attggtagtg ctgtttattt ttctcaattg caccgacgat tacaaaacgc
     2221 catttgaaaa acttttccgc ttaatcggct ggctggtatt gcttaccggt atcgtgctga
     2281 tattcgttat ctatgaatct gttcgtcctg atgcttactg gcattggttc aatacaatcc
     2341 ttgtaaccgg attgtttgct gctcagcttt ctgaaatcaa cgagctgaaa cgatccaatg
     2401 atgtgctcaa gctgcttaat ctggtggtta cgctgggtgc ttataccgtg ttgatcatga
     2461 cgatccatgg tttcccaatg aattttgtat tgttgtttat tttgttggga gttaacatgc
     2521 ttaccatgct tgcaacagtg cttaccggta aactgccctg atcatttttt ttcccaaacc
     2581 caggcatcca aacctgtttg cgatttataa cggtcaagtt cagtttgagc cttgtcttta
     2641 ctttcaaaac caccgattgc agcatattgc aatccttttc cggaatcaac cattttgcct
     2701 ttaaatcctg attgaatcaa ttccttggtt tttcttcctg cattttccga gtttttaaaa
     2761 gagcctgtta ctacgtaaaa ggtaccggtt tctgttgttg ttgcaacggc aggtttttca
     2821 tttggaagtg tgttggcaat tgatttttcg gtgaagtgct cattctgatt aacaccattt
     2881 cctgccttgg agcttgcagg tgtttttccc gatgatcctt gtatgggatg tgatgcaatt
     2941 tccttcttgc taaagagtaa attccagcct tcatacaaat cgtcgaaaac aggttgcatc
     3001 ccaaaatagc cggcaagagg aagattgcct ttcggtaatg caaggtcttt ataaaccatt
     3061 acacttcctg aataacaagc tacccaaatt tcgttggtac gttcattgaa ggtaatgccg
     3121 atcgggtttt tacctgttgc agctttttct gctactgaaa attgttccaa atcgatcttg
     3181 gaaaaagtag atgctgaata attaaccaca tataaaaacc gatctccctt gcctaatacc
     3241 attgaacgtg gagctgaacc tacaaaaagt ttctgcatgt ttcctgtatg cagattgatt
     3301 ctcgcaatgt tgccttcgtt gttcaccgat acataaagca atgaatcgcg gctatcgatg
     3361 cataaatggc gaggaccttt tcccacttcc ataaattttg aggaactcag ttcttcaaga
     3421 tcgatacgtt gaatggaagt gcctcccata acagtgacat aagcatatct gctcttcgaa
     3481 tcaattgcaa tccctctggg aaaacgccca acatgaaatc gctttaactc tttttctgtt
     3541 gagaggtcga atagggttac atctccgctg ctccaattgc tgacaagcat ttttttcccg
     3601 tcgggactaa tagccaggta ttttggaaca cttcctgcag gaataatgtt ttcaatttcg
     3661 agactctcgg tattaatctt gtagatatat gaaatatcgt attcctttcc atagcaagta
     3721 tcgcatcccg gatgctcgaa accatctccc tgcatgcaat aattgctgac ataagcatat
     3781 ttcccatcag gcgtaaaggc acattccaca ggtgcaccac gaaaactgcc ttctctgtct
     3841 tttattccaa accggtcggg ttgaattctg tcgcttattg tttttacaag ccggtaattt
     3901 tcatcgaaaa cactaattgt atggctatac atcatgtttt gcgcgaagaa taatccattg
     3961 ccattgtgaa caattgactt gggtgttaat cctccccgaa tggttttttt taaaaccaat
     4021 gtagtgtcgt tgccggcgaa tacggttgca gggataagca caatgcataa cctaaatagt
     4081 aaaatccaac agctgtttac attcctctgc attaggcaaa aatacgaata ctggcctttt
     4141 ggtttggtac ttgcctctaa aaaggagaaa ttaggcccat aaatcatttt catgaaaaag
     4201 caattattcc tccttttggc attgatagcg agtcaatttt catatgcaga ggaggggatg
     4261 tggatcccgt cgctgctgaa aaaactgaat gccaatgaac ttaaagctgc cggattgaaa
     4321 atccctgtcg aaaaaatttg gtcgacaaaa gaaagcagca taaaagatgc aattgtccat
     4381 tttggaggtg gatgtactgc tgaagttttg tcgtatgaag gtttgatcct gaccaatcac
     4441 cattgcgggt attcgcagat tcaggcgcat agttcgctgg agaacgatta cttgaagaat
     4501 ggtttttggg caatgaacag aacgcaggag ttgccaaatc ccggactcac agtttccttt
     4561 gttgttgata tggtggatgt aagcgaccag gtgatgcgtg gatgtattga tgagaatggt
     4621 cgtgtgaaca atgaaatgat taaagcaaat attgaacgtg ttcgtcaggc gaatgcgaaa
     4681 gacggttatg aaattgctgt tcgtcctttc aattatggca acagtttctt tatgatcata
     4741 acagaaactt accgtgatgt acgtctggtt ggtgcacctc cgtcgtcgat tggtaagttt
     4801 ggtggagata ctgataactg ggtttggcct cgacatacgg gcgattttac cttgtttcgg
     4861 atttatgcag gcaaggataa tcgtcctgca gattactctc ccgacaatgt gccttatcgt
     4921 cctaaatatt ctttgccggt atcgatgatg ggagttaagg aaaacgattt cacaatggta
     4981 tttggttttc ccggtactac ccagcaattt ctgcctgcag ctgctgtgga gtttttggtg
     5041 aagtatagaa atcctgcccg aataaaaatg cgcgaaacgg cactcaatat catcgatgct
     5101 gaaatgcggt cttctgattt gatccgtatt cagtattctg ccaaacaggc gaggattagc
     5161 aactattata aaaaatggat aggggaaaat aacggattga tcagattgaa tgcaatcgac
     5221 aagaaaaaag aattggagag ggaattcatc gatcgcgcaa aaagagaccg caatgatctg
     5281 ggacgatttg gtcagttacc ggatcgattt gttgatcttt acatcgcatg gcaacccttt
     5341 ggtacagcgt atgattattt ttcagaactg gtttattccg gcccggaatt tctccgctat
     5401 gcacaaggct ttgagaaatt ggtggaagcc tattccgtta aatcggtcgg taacgaaggt
     5461 aagagcctgc tggatagaat ttcagctgct gttccgggat attttaaaaa ttacaatgca
     5521 gcaaccgaca agaaattatt tgttgccctt tttcctatgt atttggatgg taccccagaa
     5581 gcactgcagc ccgatggaat tccagcaatg aagaaaaaat acaaaggaga ttggaatc
//
LOCUS       DAOMYG010000188         5631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4929_length_5631_cov_7.534971, whole genome shotgun sequence.
ACCESSION   DAOMYG010000188 DAOMYG010000000
VERSION     DAOMYG010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4929_length_5631_cov_7.534971"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..222)
                     /locus_tag="PK610_07265"
     CDS             complement(25..222)
                     /locus_tag="PK610_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35770.1"
                     /translation="MSGIDMNTNQENPKSIKETEQIQMLMSPEGSLALLALGHVGIQH
                     WREIRGKTGGKNPSVKTPTIP"
     gene            complement(231..3209)
                     /locus_tag="PK610_07270"
     CDS             complement(231..3209)
                     /locus_tag="PK610_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ35771.1"
                     /translation="MRIISTSIFILFSLAVFAGGNVFESKSSLEKTFYNNKWMPDKNY
                     QAELRELPAWQNFLQRNGDWWVMFNEESRKPHRAYGKPISVAGATMEDKARNFIQNEL
                     SGFNIPLHELELMGVAGNEKHQFVHFSQRHQGLKILESKLTVKLTNDGRVIMFGTDIF
                     SDIQLDLNSSIGNGGAINYAQTGITESVVSVVANPELFILPVPENKAYVYKLVHMVNV
                     HTIDNDLVPSDMLTLVDAHTGEILYRQNRVMHFDHKHKPPTGIDVATQGVAYPTNSYN
                     GSATLTMPNMDFTIASSPFITDAAGVISTGVPGPQPATFFLRGAWCNVRVNNVTPSFN
                     TTLNDGANVVDFTAGTIQDRSAYYHVNVVHDHCKQVLPAFAGMDFMLPTNVDLTTGNC
                     NAFYNGTSINFYALANGCTSFATVADVVYHEYGHGINDNFYSSLGSSFNNGAMNEGYA
                     DFWAYSITINPVVGLGTSTTDANSYIRRYDMNRKVYPVNLIGQVHADGEIIAGAWWDT
                     FLNLGSDMNQTIDLFADAFPGLQASALNGNEGKAFTDVLIDVLQADDDDADLTNGTPN
                     GMAIVDAFALHGITLISNAKLNHTTFGFHDAAEDIAINANLILNFPYTNYLSAVKCFY
                     KINTGAWNSGNMSNVSGNLYTIDIPAQPIGTIVYYYLSATDINNQMTAVTPIGAELTD
                     PNIPNIIMVGYALDKTDDAGDFTTELGSWQAQVPGDNASTGRWINAIPVGSYSTAGDT
                     STAVQTYYQRTLNGEFCWVTGNASSPNDALGTADVDGGHTTLRSAVIDMSGYVNPAVS
                     YYRWYINNPPSGANPNADWWQVSISNDGGTTWTFVENTKTSDRKWRRNAFRVADYVTP
                     TNNMKIRFVASDSLRPGQNLDGGSLVEAAVDDVQIWDNMDPNNVEESGVADGIQFVLR
                     PNPATDQVLVSFELYESSPIEVVLMDMSGRIIWNRSFGTVQPGISTQPVNVANVESGV
                     YQVVLRTGGKTLTRKLVIQH"
     gene            3452..4015
                     /locus_tag="PK610_07275"
     CDS             3452..4015
                     /locus_tag="PK610_07275"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRJ35772.1"
                     /translation="MKEYLEKALEVLKNGGIILYPTDTIWGIGCDAGNPEAVKRIYDL
                     KKRDDSKSMIVLVENTNRITRHVREVPEVAWDLIELSDKPLTVVFDRAYNLAPNLIGE
                     DGSIGIRVCMDEFCNQLIKKLNRPIVSTSANISGQASPRFYNDISNEIRVGVDHIVNL
                     RRNETTISQPSSIIRLKNDGQITILRK"
     gene            3984..5423
                     /locus_tag="PK610_07280"
     CDS             3984..5423
                     /locus_tag="PK610_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010520033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRJ35773.1"
                     /translation="MTVRSPFSVNKDRLQSALHHPVFQTIGTIADGCNQRVFVIGGYV
                     RDQLLGRPCKDIDLVTEGSGLELAEKVGKALGVKVNYFKNFGTAHLKYNELDIEFVGA
                     RKESYKRESRKPIVEEGTLQDDQNRRDFTINALAISLNKNNRGELIDPFDGVADLEKG
                     IIRTPLDPDITYSDDPLRMMRAIRFATQLQFSIEEKSFQSISRNKNRIEIISEERIAE
                     ELNKIILSPIPSIGFVQLFNTGLLELIFPEMAKLEGVEEVNGFSHKDNFYHTLQVLDN
                     LCRTSNDLWLRWAAILHDIAKPATKKYYPDQGWTFHGHEDLGARMVPGIFKKLKLPLD
                     HKMKYVQKLVFLHLRPIALTNGNITDSAVRRLLFEVGDDLEDLMKLCEADITTKNKAR
                     EERYLQRFQKVREVINEVEERDKIRNWQPPITGEMIMKAFNLQPCKEVGIIKNKIRES
                     ILDGEIGNNLEEATELMFTLGLEMGLKKI"
     gene            5429..>5631
                     /locus_tag="PK610_07285"
     CDS             5429..>5631
                     /locus_tag="PK610_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010522919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35774.1"
                     /translation="MRIYRFRVVLDTRDDVFCDVDINTEASFEEFYNAITNAFKFKGG
                     VMSSFYMSNENWDKGEEITLLDM"
BASE COUNT         1765 a         1136 c         1128 g         1602 t
ORIGIN      
        1 agttttttca aatataatta aaaattaggg tattgttgga gttttaacac ttggattttt
       61 tccaccggtt tttccacgaa tctcacgcca atgttgaatg ccaacatgac ctaaagcgag
      121 taaagccaaa gaaccctcag gactcataag catttgaatc tgttctgttt ccttgatgga
      181 tttcgggttt tcctgattgg tattcatatc tattccggac aaaaaattga ttaatgttgg
      241 attacaagtt tacgggtaag ggttttacct ccggttctca aaacaacttg ataaacgccg
      301 ctttcaacat tggcaacatt aaccggctgt gtgctgatac ccggttgtac agtgccaaag
      361 gagcggttcc atattattcg tcctgacata tccatcaaca cgacttctat aggtgatgat
      421 tcatatagtt caaacgaaac caaaacctgg tcggtagccg gattagggcg cagcacaaac
      481 tgtatgccgt ccgctactcc actttcttct acattgttcg gatccatatt atcccaaatc
      541 tgaacatcat caacagctgc ttccaccaat gatcctccgt caagattctg acccggacgc
      601 aaactatccg aggcaacaaa tcggatcttc atattatttg tgggggtaac gtaatcagca
      661 acacgaaatg catttcttct ccatttgcga tctgaagttt ttgtattctc tacgaaagtc
      721 caggttgttc ctccatcatt cgagattgaa acctgccacc agtcggcatt cggatttgca
      781 ccacttggag gattattgat gtaccatctg taataagaaa cggcaggatt tacatagcca
      841 gacatatcta tgactgcaga acgcaatgtg gtatgcccac catcaacatc cgcagtacct
      901 aaagcatcgt tcggagatga ggcatttccg gtaacccagc aaaattcgcc atttaatgtc
      961 cgctgataat acgtttgaac cgctgttgat gtatcgcctg ctgtagaata ggaaccaaca
     1021 ggtattgcgt tgatccacct tcctgtgctt gcattatcac ccggaacctg agcttgccat
     1081 gaacctagtt ctgttgtaaa gtcaccggca tcatctgttt tgtcgagtgc atacccaacc
     1141 ataataatgt tcggaatatt gggatctgtc aattcagctc cgataggagt taccgcggtc
     1201 atttgattat ttatatctgt tgctgacaaa tagtaataaa caatggtacc aataggttgc
     1261 gcaggaatgt caatggtata caagttaccg gaaacattcg acatattccc ggaattccat
     1321 gcacctgtat tgattttata aaagcacttt acggcactca gataattcgt gtatggaaaa
     1381 ttcaaaatca aattggcgtt gatagcaata tcttcagctg catcatgaaa accgaaagtg
     1441 gtatggttca attttgcatt tgaaataagt gtaattccgt gtaaagcaaa tgcatcaaca
     1501 atggccatac catttggagt accattagta agatccgcat catcatcgtc ggcttgtaat
     1561 acatcgatca aaacatctgt aaatgctttt ccttcattac cgttcaaagc agatgcctgt
     1621 aaaccgggaa aagcatctgc aaaaagatca atagtctgat tcatatcact accgagattc
     1681 agaaaagtat cccaccaggc accggcaatg atttcaccat ctgcgtgaac ttgcccaata
     1741 aggttaacag gatatacttt acgattcata tcgtaacgac gtatataact atttgcatca
     1801 gttgtgcttg tacctaaacc gacaaccgga ttaatggtaa tggaataggc ccaaaaatca
     1861 gcatatcctt cattcattgc accattatta aagctactgc ccagtgaact gtaaaaatta
     1921 tcattgattc catgtccgta ttcatggtaa acaacatcag caaccgttgc gaatgaggta
     1981 cacccgttgg caagtgcgta aaaattaatg gaagttccat tgtaaaaagc gttgcaattt
     2041 ccggtagtta aatcaacatt cgtgggtagc ataaaatcca ttcccgcaaa tgccggaagc
     2101 acttgtttgc aatgatcatg tacaacatta acatggtaat aagcggaacg atcctgaatt
     2161 gtacctgccg taaagtctac aacattggcg ccgtcattta atgtggtatt aaaacttggt
     2221 gttacattat tcactctgac attgcaccat gcaccacgaa gaaaaaatgt agcgggctgt
     2281 gggcctggta caccggttga tattacacct gcagcatccg taataaaagg agatgatgct
     2341 attgtaaaat ccatattggg catagtaagt gttgccgaac cattatagga attcgttggg
     2401 taagcaactc cctgcgtagc cacatcaata ccggttggtg gtttgtgctt gtgatcaaaa
     2461 tgcattaccc tgttttgtct gtacaagatc tcaccggtat gcgcatctac gagagtcaac
     2521 atatcagaag gaacaagatc attgtcaatc gtatgcacat taaccatatg tacaagttta
     2581 tagacatagg ctttgttttc aggaacgggc aaaataaata actcaggatt tgcaacaacg
     2641 gatacaaccg attccgtaat acctgtttgc gcgtaattga tagcgccacc attaccaatg
     2701 gatgaattta gatcaagctg aatgtcggaa aaaatatccg taccgaacat tataacacga
     2761 ccgtcgtttg taagtttcac tgtcagttta ctctccagaa tttttaatcc ctggtgacgt
     2821 tgtgaaaaat ggacaaactg atgtttttca tttcctgcaa cacccattaa ttcgagttca
     2881 tgcaacggaa tattaaaccc actaagctcg ttttgaataa agtttcgcgc tttatcctcc
     2941 attgtagctc ccgcaacaga aataggtttt ccgtaagcac ggtgtggttt tctgctttct
     3001 tcattgaaca tcacccacca atcaccattg cgttgcaaaa agttttgcca ggctggtaat
     3061 tcgcgcaatt cggcctgata gtttttgtca ggcatccatt tgttgttgta gaaggttttt
     3121 tcaagcgacg atttgctctc aaaaacattt ccaccggcaa acactgccag agaaaaaaga
     3181 ataaaaatgg aggtagaaat aatcctcata gaatcttccg gttggttaag ggctttaaat
     3241 tcatcaaata tctgcccgta accaaaccgt atcctaaata ttatcgaatt caacccattt
     3301 tgtttaaaac gagaattctc cttggtaaga atctcatgat gattactcga aaactcctaa
     3361 ttatccaact gaaatacaaa tgtttacatc ttaattgttg atttttttgg atgcgaagtg
     3421 atttaggact ttctctctac ttttgcagcc catgaaagaa tatctcgaaa aggcacttga
     3481 agtcctgaaa aacggaggaa ttatcctcta cccgaccgac accatctggg gtattggatg
     3541 tgatgctggt aacccggaag ctgtgaaaag gatttacgat ttgaaaaaac gtgacgatag
     3601 caaaagcatg atcgttttgg tggaaaacac aaaccgcatc acacgacatg taagggaggt
     3661 accggaagtt gcctgggatt taatcgaatt gagcgataaa ccattaacgg tggtctttga
     3721 tcgggcctac aaccttgcac ctaacctaat aggcgaagat ggttcgatag gtatacgtgt
     3781 ctgcatggat gagttttgca atcaattgat aaaaaaattg aaccgtccaa tcgtttcaac
     3841 ctctgcaaat atctccggtc aggcatcacc acgtttttat aatgacatca gtaatgaaat
     3901 tcgcgttggc gttgatcata ttgtaaattt gcgccggaat gaaacaacga tttcacaacc
     3961 ttcttcaatt atccgcttaa aaaatgacgg tcagatcacc attctccgta aataaggacc
     4021 gtctgcaatc tgcgttacac catccggtat tccagacaat tggaaccata gccgatggtt
     4081 gtaatcagag agtatttgtt attggtggat atgttagaga tcaactgctg ggcagaccat
     4141 gcaaggatat tgatctggtt acagaaggaa gtggacttga attggcagaa aaggttggca
     4201 aagcgctagg ggttaaagtg aattacttta aaaatttcgg gacagctcac ttaaaataca
     4261 atgagctcga tatagaattt gttggtgcgc gaaaagaatc ctacaaaaga gaatcgcgta
     4321 aaccaattgt tgaggaagga acattacagg acgatcagaa ccgccgtgat tttacaatca
     4381 atgcgttggc aatttccctc aataaaaata accgtggaga actgattgat ccttttgacg
     4441 gagttgcaga tctggaaaaa ggaattattc gcaccccact cgatccagac atcacttatt
     4501 ctgatgatcc attgcggatg atgcgggcaa tacgttttgc aacccaactt caattttcaa
     4561 ttgaagagaa atcatttcaa tcaatttcaa gaaataaaaa caggatagaa attatttcgg
     4621 aagaacgaat tgcagaggaa ctcaataaaa tcattctttc tccaattccc tctatcggtt
     4681 ttgttcaatt gttcaataca gggttacttg agttgatttt tcctgaaatg gcaaaacttg
     4741 agggagtaga agaagtgaat ggttttagtc ataaggacaa tttttaccat acccttcagg
     4801 tcttggataa tttgtgccgc acatccaacg atttatggtt gcgatgggct gcaatactgc
     4861 atgatattgc taaaccggca acaaaaaaat attaccctga tcagggatgg acttttcacg
     4921 gacatgaaga tttaggcgca cggatggttc cgggaatttt taaaaagtta aaactacccc
     4981 tggatcataa aatgaaatat gtccaaaaac tcgtgtttct ccatttaaga ccaatagctc
     5041 ttactaacgg aaacattacc gattctgcag tacgacggtt gctttttgag gttggtgatg
     5101 acctggagga tctaatgaaa ttatgtgaag cagatatcac tacaaaaaac aaagccaggg
     5161 aagaacgata cctacagcgc tttcaaaagg taagagaggt tattaatgaa gttgaagagc
     5221 gggataaaat tagaaactgg cagcctccta tcaccggaga aatgataatg aaagctttta
     5281 atttacaacc gtgcaaagaa gttggaatca taaagaataa aataagagaa tctattctag
     5341 acggtgagat aggaaacaac ctcgaagagg ccactgaatt aatgtttact ttaggtcttg
     5401 aaatgggcct gaaaaaaatc tgataaccat gagaatttac cgattccgag tagtactcga
     5461 cacaagggat gatgtttttt gcgatgttga catcaataca gaagccagtt ttgaagagtt
     5521 ttataatgcc attaccaatg cctttaaatt taaaggaggt gtaatgtcaa gtttttacat
     5581 gtcgaacgaa aactgggata aaggcgaaga aatcacccta ctcgatatga g
//
LOCUS       DAOMYG010000189         5623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4936_length_5623_cov_8.418642, whole genome shotgun sequence.
ACCESSION   DAOMYG010000189 DAOMYG010000000
VERSION     DAOMYG010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4936_length_5623_cov_8.418642"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PK610_07290"
     CDS             <1..547
                     /locus_tag="PK610_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRJ35775.1"
                     /translation="AENVNLPIILYNVPGRTGSNMRAALTLRIAREIPGIVAIKEASG
                     DLNQIMEIIANRPDGFLVYSGDDAMTLSIIAAGGDGCISVVANQVPSEFSRMIHAALA
                     NDLDLARNLHYKLLRLMNLNFIESNPIPVKTCLYMMGLLQNEFRAPMISMTNSSNLQV
                     LHEELRTLEIAVNDRSAELIA"
     gene            578..1903
                     /locus_tag="PK610_07295"
     CDS             578..1903
                     /locus_tag="PK610_07295"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019385985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRJ35776.1"
                     /translation="MNVMKFGGTSVGSPQRMKEVAAIIDRGENVVVVLSAVSGTTNKL
                     VDIGHAYSSNEKQKALDIINVLQSSYSVFLDELFSTNEGKTAGTEIIQNHFDLLRSFS
                     RNGFGVRQYKEILAQGEMISTQLFFQYMKECGKDAVLMNALDFMRIDEDGEPVMEQIK
                     RSLAQLLSQNTARFVITQGFICRNPLGEIDNLKRGGSDYSASLIGAAIGADEIQIWTD
                     IDGMHNNDPRIVEGTFPVRQLSFDEAAELAYFGAKILHPSSVNPARSAGIPVRLKNTM
                     KPDAPGTLIAEHASEPGTIKAIAAKDGIIAIRIVSNRMFLAYGFLRRVFEIFERYKTP
                     IDMITTSEVAVSLTIDQDVNLDQILSELNEIASVEIDRDLSIVCVVGDNLESESGFAG
                     RILAGLQDLPVRMVSCGGSKNNVSLLIASSNKNHALNLLNSTLFTKVTA"
     gene            1900..3081
                     /gene="lysA"
                     /locus_tag="PK610_07300"
     CDS             1900..3081
                     /gene="lysA"
                     /locus_tag="PK610_07300"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRJ35777.1"
                     /translation="MMFSKKLTSTFAEHRTPFYYYDIDLLEKTLQVLRKESDKFGFHV
                     HYAFKANCNDSVLQTILRSGLGADCVSGGEVQKALDAGFPGSRIVFAGVGKTDEEIEL
                     GIDASIFCFNCESVPELQIIQEIAERKGKKVDVALRINPNVNANTHKYITTGLEENKF
                     GISISQLPDVLEVLAECANLRLAGIHFHIGSQITDLTSFRNLCTRINEIQQWFRSRNI
                     SLEHINVGGGLGVDYYAPDEKPIAEFAAYFSLFNEFLDLNPGQQVHFELGRAIVAQCG
                     SLISKVLYVKNGLNTDFAIVDAGMTELIRPALYQSYHKIENLSGQLRNDKLAYYDVVG
                     PVCESSDCFGKRVELPSTQRGDVFAIRTAGAYGEVMASNYNLRGKVRSFTSADLLKEK
                     A"
     gene            complement(3082..5400)
                     /locus_tag="PK610_07305"
     CDS             complement(3082..5400)
                     /locus_tag="PK610_07305"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ35778.1"
                     /translation="MLISSALFAQTATIRGFVYDKATGEPISYANILIKGTGLGASAD
                     GNGFFQISKVTPGTYTIIISFVGYQPIQEEITLKAGEIFNKKFLLTEGTELAEIEVNA
                     EKQESLTAIKMSAIKATRKEINAVPTIGGESDIATYFQTVPGVVTTGDQGGQLYVRGG
                     APIQNKVLLDGMTIYNPFHSIGFFSVFDTDIIRTADIYTGGFNAEYGGRISSVMDITT
                     RDGNKNKFEGKVAASPFGAKLMLEGPLKKAKKPGGSTASYLISTKTSYLAQTSKILYK
                     YVDTAGLPFNFTDIYGKLSFNGSNGSKLNLFAFNFADSVKYQAVSDLKWNAFGVGSNF
                     VLVPEGNPVLISGDFSFSNYRITLDDKISPVRSSEVNGFNLGFDFKYFLKLDEVKYGI
                     EVEGFTTDFITYNALNREIRQEQNTTQIGAYVSYKLSRGRLLLEPSMRTMYYASLSSF
                     SPEPRIGAKFNLTEDIRFKAAAGVYSQNLIAANSDRDVVNLFYGFLSGSEDLPKKYVD
                     EKGNVVDRKHPLQKANHYIFGVEYDLNKYLTFNVEGYLKDFTQLTNINRNKIYNSDDA
                     TKPDILKKDYIVETGRAYGVDFVSKFNTKKTYLWVVYSLGKVTRWDGIQTYAPIFDRR
                     HNINMVFTQLMGKEDLWEVNARWNYGSGLPFTPTGGYYMNVNFLDVNTDYTTANSNDL
                     SLIYSSLNSGRLSDYHRFDITFKRRFEFKKVKKATEEGKPDETKITSKLELIASATNI
                     YNRRNIFYVERVTGQRVYQLPIIPTLGFNWAF"
BASE COUNT         1608 a         1081 c         1249 g         1685 t
ORIGIN      
        1 cgcagaaaat gtaaacctgc ctatcattct ttataatgtt ccgggacgta caggttcgaa
       61 catgcgtgca gcactcacgc ttcgcattgc ccgcgaaatt ccaggaattg tggctatcaa
      121 agaggcttcg ggcgacctca accagatcat ggaaattatt gccaatcgtc cggatggatt
      181 tttagtttac tccggcgatg atgcgatgac actttcaata attgctgcag gtggtgatgg
      241 atgtatttcg gtggtggcca atcaggtgcc ttcggaattt agtcgtatga ttcatgctgc
      301 attggcaaat gatcttgatc ttgcacgtaa cctgcattac aaattgctgc gtttgatgaa
      361 tcttaatttt atagaaagca atcccattcc ggtgaaaacc tgtttgtata tgatgggttt
      421 attgcaaaac gaatttcgtg caccaatgat ttcaatgaca aattcttcca acctgcaggt
      481 attgcatgaa gaattgcgta cacttgaaat agcagtgaac gatcgttcag cagaattgat
      541 cgcctgaatt ttagtagaac ggaaataaga tacagccatg aatgtgatga agttcggggg
      601 gacctcagtg gggtctcccc agcgtatgaa agaagttgcc gcaattattg atcgtggcga
      661 aaatgtagtg gtcgttctct cggccgtttc gggaaccact aataaactgg tggatattgg
      721 tcatgcttat tcctccaatg aaaagcaaaa agcgttggac atcatcaatg tgcttcagtc
      781 ttcctattct gtttttctcg atgaattatt ttctacaaat gaaggaaaga cagcaggaac
      841 tgaaattatt cagaatcatt ttgatctctt gcgttccttt tcgcggaatg gttttggtgt
      901 tcgtcagtat aaagaaattc ttgcccaggg agaaatgatc tctactcagt tgttttttca
      961 atacatgaaa gaatgtggta aagatgcagt gttgatgaat gccctggatt ttatgcgcat
     1021 tgatgaagat ggtgaacctg ttatggaaca gatcaaacgt tctcttgcac aactgctgag
     1081 ccaaaatact gcacgtttcg ttattaccca gggtttcatt tgcaggaatc ctctcggaga
     1141 aattgacaac ctcaaacgtg gaggcagcga ttattctgct tcactgattg gtgctgccat
     1201 tggtgcggat gagattcaga tctggacaga cattgatgga atgcacaata acgatccccg
     1261 tattgttgag ggtacatttc cggttaggca attatcgttc gacgaagcgg cggagcttgc
     1321 ctattttggt gcgaagattt tgcatcccag tagtgtaaat cctgcccggt ctgcaggaat
     1381 tccggtgcga ttgaaaaata ccatgaagcc ggatgcaccc ggaacactta ttgctgaaca
     1441 tgcttctgaa ccgggaacca taaaagcaat cgctgcaaaa gatggaatca tcgcaatacg
     1501 tattgtctct aacagaatgt ttctggcgta tggatttttg cgaagagtgt ttgaaatatt
     1561 tgaacgctac aaaacaccta tcgatatgat tacaacatcc gaagttgcag tatcactcac
     1621 gatcgatcag gatgtaaatc ttgatcaaat tctctctgag ttaaatgaaa ttgcctcagt
     1681 agaaatcgat cgtgacctga gtattgtttg tgttgttggc gataatcttg agagtgaaag
     1741 cggatttgca gggagaattt tggcaggact tcaggatttg cctgtgcgta tggtttcttg
     1801 cggcggatca aaaaacaatg tgtctttgct tatcgcttct tccaataaaa accatgcact
     1861 taatttattg aattctacat tgttcactaa agtcaccgca tgatgtttag caaaaaactc
     1921 acctcgactt ttgcggaaca tcgcacgcca ttctattatt acgatatcga tttgcttgaa
     1981 aagacacttc aggtcctcag gaaggaatct gataaatttg gatttcatgt gcattatgca
     2041 tttaaagcaa attgtaacga ttccgtttta caaactatcc ttcgttccgg cttaggcgca
     2101 gattgtgtta gtggcggaga ggtgcaaaaa gcattggacg caggatttcc cggaagtcgg
     2161 attgtatttg caggtgtagg taaaacggat gaagaaattg aactgggaat tgatgcttcc
     2221 attttttgtt tcaattgcga atctgttcct gaattgcaaa tcattcagga aattgctgaa
     2281 cggaaaggaa aaaaagtgga tgtagcatta cgcatcaatc ccaatgtgaa tgccaatacg
     2341 cacaagtata ttactacagg actggaagaa aataaattcg gaatcagcat ttctcaattg
     2401 ccggatgtac ttgaagttct tgccgaatgt gcgaatttac gtttggcggg aattcacttt
     2461 catattggct cgcaaattac tgatctcact tccttcagga atttgtgtac gcggataaat
     2521 gaaattcaac aatggtttcg ttctagaaat atctcccttg aacatatcaa tgttggaggt
     2581 gggctcggtg ttgattatta cgctcccgat gaaaagccta ttgccgaatt cgcagcttat
     2641 ttctccttgt tcaatgaatt tctcgatctg aatccgggac agcaggtgca tttcgaattg
     2701 ggaagagcca ttgtcgcgca atgtggctcg cttatatcga aagtactgta tgtaaagaac
     2761 ggactgaata ccgattttgc aattgtcgat gcaggtatga cggagttaat ccgtcccgca
     2821 ttgtatcagt cgtaccacaa aattgaaaac ctcagcggac aattacgtaa tgataaattg
     2881 gcgtactacg atgttgtagg gcctgtttgt gaatcgtccg attgttttgg gaagcgtgtc
     2941 gaattgcctt caacacaaag aggcgatgta tttgcaatac gaactgcagg tgcttacgga
     3001 gaagtaatgg cttcgaatta caacctcagg ggaaaagtaa ggtcttttac ttccgccgat
     3061 ttgctaaagg aaaaagcctg atcagaatgc ccagttgaat ccgagtgttg ggataatggg
     3121 taactgatac acacgctgac cggtaacgcg ctctacgtag aaaatatttc ttctgttgta
     3181 gatattggtt gccgatgcga taagttccag tttcgaagtg attttcgttt cgtcgggttt
     3241 gccttcttca gttgctttct tcactttctt gaattcgaag cgacgcttaa acgtaatgtc
     3301 gaaacgatgg taatcgctca aacgtccgga attaagtgaa ctgtagatga gcgaaagatc
     3361 attggagttg gcggtggtgt aatcggtatt tacatcgagg aaattcacat tcatgtaata
     3421 tcctcctgta ggagtaaatg gtaatccgga accgtaattc caacgtgcgt taacttccca
     3481 taaatcttct ttgcccatta attgggtaaa gaccatgttg atgttgtgac gacgatcaaa
     3541 aatcggagcg taggtttgaa ttccatccca gcgggttact ttccctaaag aatatacaac
     3601 ccacaaatat gttttttttg tattgaactt cgatacaaaa tccacaccgt atgcacgacc
     3661 agtttcaacg atataatctt tcttgaggat atcaggtttt gttgcatcgt cggaattgta
     3721 aattttatta cggttgatat tggttaattg tgtgaagtct ttgagatatc cctccacatt
     3781 aaaggtcagg tatttgttga ggtcatactc aacaccgaaa atatagtgat tggctttttg
     3841 caaggggtgt ttgcgatcaa caacatttcc tttttcatca acatattttt tcggaagatc
     3901 ttcacttcca ctgaggaaac cataaaatag attcacaaca tcacgatcgg aattggctgc
     3961 gattaggttt tgtgaataca caccagctgc tgctttgaaa cgaatgtctt cagttaaatt
     4021 gaatttagct ccaatgcgag gttccggaga aaaggaggaa agtgatgcat aatacatcgt
     4081 tcgcatactg ggctccagta ataaacgtcc gcgtgaaagt ttgtagctga cataagcacc
     4141 gatttgcgtt gtattctgct cctgacggat ctcgcggttc aatgcattgt aggtaatgaa
     4201 gtcagtagtg aatccttcaa cttcaatacc gtattttact tcatccagtt ttaggaaata
     4261 cttgaagtca aaaccaagat taaaaccatt tacctcactg cttcttaccg gagaaatttt
     4321 gtcgtccaac gtgatcctgt aattggaaaa agaaaaatca ccggaaataa gaaccggatt
     4381 tccctcagga accaatacaa agtttgaacc tacaccaaag gcattccatt tcaaatcaga
     4441 aacagcctgg tattttacac tatcagcgaa attgaaagcg aaaagattca gcttcgaacc
     4501 gttgctacca ttgaaggata attttccata gatatccgta aagttgaatg gcagtcctgc
     4561 tgtgtcaaca tacttataaa gaatcttgga cgtctgagca aggtaggagg tctttgtaga
     4621 aattaaatat gaagcagtag atcctcccgg tttttttgct tttttaagcg gaccttcaag
     4681 cataagtttt gctccaaaag gactcgcagc cactttacct tcaaacttat ttttgtttcc
     4741 gtctcgggta gtgatgtcca ttaccgatga aattcttcca ccatactctg cgttaaatcc
     4801 tccggtgtaa atatcagctg tgcgaataat atcggtgtca aacacagaaa agaaaccaat
     4861 ggagtggaat ggattataaa tcgtcattcc atcaagcagt actttgtttt gaatgggagc
     4921 accaccacga acatacagct gtcctccctg gtcaccggtt gttacaaccc ctggtactgt
     4981 ttggaaatag gttgcaatat ccgactcccc accaatggtt ggaacagcat tgatttcctt
     5041 tcttgtagcc ttaatagccg acattttaat tgcggtaagg gactcttgtt tttcggcatt
     5101 cacttcaatc tctgcaagtt ctgttccttc agtcaaaagg aattttttat tgaagatttc
     5161 acctgccttt aatgtgattt cttcctgaat gggttgataa ccgacaaacg aaatgataat
     5221 tgtgtaagtg ccgggcgtaa ctttagaaat ctggaagaaa ccatttccat cagcggatgc
     5281 acccaatcct gtacctttta taagaatgtt agcatagctg attggttctc ctgttgcctt
     5341 atcgtaaacg aatccacgga tagtggcagt ttgcgcaaat agggctgacg aaatcagcat
     5401 gaaaacgata attagcgagg aacggaagga gtatgtcatg atataaatag gaaagcgacg
     5461 aaattaacaa tattctgcaa ggtgtcagga caaatcgctc ttcaatttgg gaaaggtagg
     5521 aggactgact tttacggtaa aggtggggag taatgaacag gggggctgtt caaatcattt
     5581 tttgaggttt ttgctagatc gaaagagtgt cgtgtaggga aag
//
LOCUS       DAOMYG010000190         5621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4940_length_5621_cov_6.902264, whole genome shotgun sequence.
ACCESSION   DAOMYG010000190 DAOMYG010000000
VERSION     DAOMYG010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4940_length_5621_cov_6.902264"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..1581
                     /locus_tag="PK610_07310"
     CDS             232..1581
                     /locus_tag="PK610_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ35779.1"
                     /translation="MRGVLLVFFLINNLLHAQVQPCDKAISQLPGIEERAVKGEWETA
                     LSLCNTAMKSCPDRGEFLLLRADLFLKLDNIDSAEIDLRRYIFLFPNEGTGFRKMAGL
                     HYFIGGIDSSLHYLDRALKINAADTIALYNKGEVWFGERELDKAEKQYLHLLKIAPDH
                     LAARLRLCDIWISQDKTSACREQLEIMQRNHAQNLDVILTDAMLLIREEHFDRAAVVL
                     DNAQRIDSTEKAIYFTRALLYERTNKYSEALRQYNKIITIDPDDANAYFERGNLLIEM
                     DQFELAIADYKDVLSIDTSFADAWHQIGIALYELSDYEGSVKAMDEYLKRNPEDAEAW
                     FNRGNGKYAHQDYSDALIDYNEALNREENSDYRYNRAICHYALGNPSAAIRDLDEYLL
                     KESADAEGYYLRCLCHLEIQQFEDGCSDCEMARKMGKKDIPKNINKICNGKHRKKRK"
     gene            1553..2773
                     /locus_tag="PK610_07315"
     CDS             1553..2773
                     /locus_tag="PK610_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HRJ35780.1"
                     /translation="MESTERNENNEQELHDDSFYQQIAKTYHGLEELLADEIRELGGT
                     DVLVLNRAVSYSGDDELMYKVNLHSRTALAVLVPIETFKAEDPDDLYEKAMKVEWENY
                     FSCNDSFAIHHSINSDFFTHSQFAALKLKDAIVDRFRKVEGRRPSVDKENPQFRINIH
                     IFKQEVTLSFDSSGEPLFKRGYRQRQSQAPLNEILAAGMIMFSGWKGEKTFIDPMCGS
                     GTLPIEAAMIAMNIPAGWYRRRFGFMNWNNYDERLWNRIKDEAREKILKQPPHLIIGS
                     DILSENIDKCERNAHLIRCNQLKFAVRDFFDFQRPDNEAALVMLNPPYGERLDNEPEL
                     ENFYKAIGDEFKKKFTGCDAWLISSNMEAIKKIGLKADQKITLYNGPLECKLLHFPLF
                     EGSQKEKQGKVNGN"
     gene            2855..4351
                     /locus_tag="PK610_07320"
     CDS             2855..4351
                     /locus_tag="PK610_07320"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRJ35781.1"
                     /translation="MSTVFRKIFANASTRILILVFAVIIGITSYFLVTGYYTQLDLYE
                     KAELNKLRGIANTLARQIDGTAHDILYQQYPKKDQIQTTSQDPVYLEFHRILEEAHDA
                     NELETAIYTIVYEPKEKIFRFAVFSDTVYWRHEWEKFHDTHVQQYETGGIIHPYTDEN
                     GTWLSAFHPIRDHKGKVVAVVQVDSRFEPFILQTRKEIGIRAGISLAIIGLIGFVLFR
                     SIRGILKKEEQMTNEIIQSKEIIEAKNKDITDSIIYAKRIQEAILPSVDHIREQLKDS
                     FVLFLPRDIVSGDFYWYTDHADRVYIAAVDCTGHGVPGAFMSMIGNTLLNEIINQKDV
                     RDPGKALDLLDAGIRKALKQNDDNTETRDGMDLALVSLCKHFKDLTFSGAFRPLLHIR
                     GDELTEHRANRFPIGGGNAYVKTPFTTTTVQLQSGDLIYLFSDGYPDQIGGEGDKKFM
                     TRRFKELIMEHKNLPMAQQEKILLKSLQDWQGEHEQMDDILVIGIRVP"
     gene            4364..>5621
                     /locus_tag="PK610_07325"
     CDS             4364..>5621
                     /locus_tag="PK610_07325"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRJ35782.1"
                     /translation="MRVIIFFLTLFSIPSLHSQSIGIYFPEIVFDFGYVMEAEGKVYH
                     TFHFSNNGKETVEIKEIATSCGCMLPRASGKKLEPGEKGFIQVEYDPEGRPGKFIKSI
                     EIVFSSSSVSEQKFYLNIKGIVIGKEVLPAFKEDWKQTQLQIKPFKAVIVSASDFRFL
                     NQPELQTFINDITYEIDLNGFSTVELEILIPKGKKLSEPADQLFLPAKKYLISELRRR
                     NYSQNQIGFLEKGPTPTSQINSDAMGMIRIASRDLNNDSIPESGFFFRHTDQRNHFRE
                     LEKLQRSEDSIKAVRSGIRNYSQGTIKSIDLKSDHYRSFLEQVVRQALINNTIVIAAR
                     IESAALPDKIEKEKIALKKQLLVLSRQFLNSCREEGIPGEKIIFSVPAIIVHELSEKK
                     LPEKKLSLTLFTRFSQEDHFDLVELLA"
BASE COUNT         1758 a         1148 c         1174 g         1541 t
ORIGIN      
        1 ggtcgaatac accacatcta ccctgtcctt tttacttttc gacatgcttc tttgttttgt
       61 ctaaaaatcg taattcaaca ggttcagact cattgagaaa aaacattttt tccacagatc
      121 actagggata atggatatca acaagggaaa atccggatta cgcgttgttt tttcacacac
      181 gattcaccct aaaaacacgt ttaccgcagg aaagccgtat ttttgccgac catgagagga
      241 gtccttcttg tatttttcct gattaacaat ctgttacacg cacaggttca accctgcgat
      301 aaagccatat cccaattacc cggaattgaa gagcgggctg taaaaggtga atgggaaact
      361 gcattatcct tgtgcaatac cgcaatgaaa agttgtcccg accgcggtga atttctcctt
      421 ctgcgagccg atcttttttt aaagcttgac aatatcgatt ctgctgagat tgatttacgt
      481 cggtatattt ttctttttcc aaatgaagga acaggatttc gaaagatggc cggattgcat
      541 tatttcattg gaggaatcga ttccagtttg cattatctgg atcgtgcctt gaaaataaat
      601 gcagccgaca ccattgcatt gtacaataaa ggagaagttt ggtttggtga gcgggaattg
      661 gataaagcag aaaagcaata tttgcattta ctaaaaatcg cgcctgatca tttagcagcc
      721 cggttaagat tatgcgatat ttggatttcg caggacaaaa cttctgcatg cagggagcag
      781 cttgaaatca tgcaaagaaa tcatgcacaa aacctggatg taattcttac tgatgcaatg
      841 ttgctcatcc gcgaagagca tttcgacaga gcagcagttg ttcttgacaa tgcccagagg
      901 attgattcaa cagagaaggc catttatttt acccgtgctc ttctttatga gcggaccaac
      961 aaatactccg aggcacttcg tcaatacaat aaaattatca ctatcgatcc ggatgatgca
     1021 aacgcctatt tcgaacgggg aaatctgttg atagaaatgg atcagtttga acttgcaatt
     1081 gcagattaca aggacgtatt atcaatcgac acctcctttg ctgatgcatg gcaccaaata
     1141 ggaattgcac tttacgagtt aagtgattat gaaggatctg taaaagcaat ggatgaatac
     1201 ctgaaacgca atccggaaga tgcagaagca tggtttaacc ggggtaatgg taaatatgct
     1261 caccaggatt attcggatgc attaatcgat tacaacgaag cactcaatcg cgaagaaaat
     1321 tccgattaca gatacaatcg tgcgatttgc cactatgctc taggtaaccc gtctgcagca
     1381 attcgtgacc tggatgagta tcttctcaaa gaatcggctg atgcagaagg atattatctg
     1441 cgttgtttat gtcaccttga gattcaacag ttcgaggacg gttgttccga ctgcgaaatg
     1501 gctcgtaaaa tgggtaaaaa agatatccca aaaaacatca ataaaatctg caatggaaag
     1561 cacagaaaga aacgaaaata acgaacagga gctgcatgat gattcctttt accagcagat
     1621 tgcaaagact tatcatggtc tggaagaatt gctagcagac gaaattcggg aattgggagg
     1681 gacggatgta cttgtattga accgagctgt ttcctattca ggagatgatg aattgatgta
     1741 caaagtcaat cttcattcac gtactgcgct tgctgttttg gttccaattg aaacgttcaa
     1801 agctgaagat ccggatgatc tgtatgaaaa agcgatgaag gttgaatggg aaaattattt
     1861 ttcctgtaat gattcctttg cgattcacca ttcgattaac tcagattttt ttacgcatag
     1921 tcagttcgct gcacttaaac tgaaagacgc cattgttgac cgattcagaa aagtcgaagg
     1981 tcgtcgtccg agtgtcgaca aggaaaatcc acagttccgt atcaacatcc atattttcaa
     2041 acaggaagtc acactatcat tcgacagcag cggagaacca ctgtttaaac gcggataccg
     2101 tcagcgccag agtcaggctc cgctcaatga aattcttgca gcaggaatga tcatgtttag
     2161 cggatggaaa ggagaaaaaa catttatcga tccgatgtgc ggaagcggaa cattacccat
     2221 tgaagcagca atgatagcaa tgaatatccc cgcaggctgg tacagaaggc gttttgggtt
     2281 tatgaattgg aacaattacg acgaacgcct ttggaacagg attaaagatg aggcaagaga
     2341 aaagatcctc aaacaaccgc ctcacctgat tattggaagc gatatcctaa gtgaaaacat
     2401 cgataaatgt gaacgcaatg cccatttgat tcgatgcaat cagctaaagt ttgctgttcg
     2461 cgatttcttt gatttccagc gtcccgataa tgaagcagca cttgtaatgc taaatccccc
     2521 ttatggtgaa aggctggata acgaacccga acttgagaat ttttataagg caattggtga
     2581 tgaattcaaa aagaagttca ccggttgtga tgcatggctg atcagctcaa atatggaggc
     2641 aattaagaaa atcggattaa aagccgatca gaaaatcacc ctgtataacg gtcctctcga
     2701 atgtaaactc cttcatttcc ccttatttga aggttctcaa aaggaaaaac agggaaaagt
     2761 taacggaaac taaatcgtta ctgcttgaat ttcaatgaaa acttgtcctt atatcgcaga
     2821 tttttagtag aattgtaagc caaacaccct tgccttgagt acagttttca ggaaaatatt
     2881 tgccaatgcc tccacacgta ttctcatact cgtatttgct gttattattg gcatcacttc
     2941 ctattttctg gttaccggtt actataccca gcttgatctt tatgagaaag ctgagctcaa
     3001 caaattacgg ggaatagcca atacactcgc tcgtcagata gatggaactg ctcatgatat
     3061 tctttatcag cagtatccga aaaaagacca gattcagacc acatcacaag accctgtata
     3121 tctggaattc caccgaatac ttgaagaggc acacgatgct aatgaacttg aaacagccat
     3181 ttacacgatt gtatacgaac caaaggagaa gatttttcgc tttgcagttt tttcagatac
     3241 ggtttactgg cgacacgaat gggagaaatt ccacgatacc cacgtgcagc aatatgaaac
     3301 cggtggcata attcaccctt acacggatga gaatggcacc tggctttcgg cctttcatcc
     3361 tattcgcgac cataagggaa aagtagtagc agttgttcag gttgattccc gctttgagcc
     3421 ttttattcta caaaccagaa aagagattgg aattcgtgca ggaatatcat tggctattat
     3481 tggtctgatt ggttttgttc tcttccgatc tattcgcgga atattgaaaa aggaagaaca
     3541 gatgaccaat gaaatcattc agagcaagga gatcattgag gcgaagaaca aggatattac
     3601 agacagtatt atctacgcaa aaagaattca ggaagccatt ctgccctcgg ttgatcacat
     3661 tcgtgaacaa ttgaaagatt cgttcgtatt gttccttcca cgcgacattg tgagcggtga
     3721 tttctattgg tacaccgatc atgccgaccg tgtttacatt gcagcagttg actgtactgg
     3781 tcacggagtt ccgggtgcgt tcatgagcat gattggaaat acgctgctga acgaaatcat
     3841 caaccagaaa gatgttcgcg atcccggcaa agcgttggat ctgttggatg ctggaataag
     3901 aaaagctctt aagcaaaacg acgacaatac ggaaacacgc gatggtatgg acctcgcttt
     3961 ggtgtcattg tgtaaacact ttaaagacct tactttctcc ggagcatttc gtccactttt
     4021 gcatatacga ggtgatgaat tgaccgaaca tcgtgccaac cgtttcccta tcggcggagg
     4081 aaatgcctac gtaaaaacgc catttaccac cacaactgtt caacttcaat cgggcgattt
     4141 aatctatctt ttctccgatg gataccctga tcagattggt ggcgaaggcg ataaaaaatt
     4201 catgacccgc agattcaaag agctgatcat ggaacataag aatctcccca tggcccagca
     4261 ggaaaaaatc ctgttgaaat cacttcagga ctggcaggga gaacatgaac agatggatga
     4321 catcctcgtt atcggaatcc gggttcccta atcattcgtt ggtatgcggg tgatcatctt
     4381 cttcctgaca cttttttcca tcccgtcttt gcactctcaa agcataggaa tttatttccc
     4441 tgagatcgtt tttgattttg gctatgtgat ggaagccgaa ggaaaagttt atcacacctt
     4501 tcattttagc aataacggaa aagagacggt cgaaataaaa gagattgcaa cgtcatgtgg
     4561 ctgtatgctt ccccgggcat caggaaaaaa gctggaacct ggtgagaaag gatttattca
     4621 ggtcgaatac gatcccgaag gacgtccggg aaaattcatc aaatccattg agatcgtttt
     4681 ttcctcttcc tcagtttcag aacaaaaatt ttacctcaac ataaagggaa ttgttattgg
     4741 gaaagaagta ttaccggcct ttaaagaaga ctggaaacaa acccaattgc aaattaaacc
     4801 ctttaaagct gttattgttt cagcttccga ttttcgcttt ttgaaccagc cggagcttca
     4861 gactttcatc aacgatatca cttacgaaat tgacttgaac ggattcagca ctgtcgagtt
     4921 ggaaatatta atccctaaag gaaaaaagct atcagaaccg gcagatcaat tgtttcttcc
     4981 ggcaaaaaaa tacctgattt ccgaattacg aagaagaaat tattctcaga atcaaatcgg
     5041 ttttcttgaa aagggaccta cacccacttc tcaaataaac tcagatgcga tgggaatgat
     5101 ccgtattgca tcaagagatc tcaataacga ttccattcct gaatccggtt tcttttttcg
     5161 tcataccgat caacgcaacc acttcaggga actggaaaaa ctgcaacgta gcgaagactc
     5221 aataaaggca gttcgaagcg gaatcagaaa ttattctcag ggaacaataa aatcgattga
     5281 tttaaaatca gatcattacc gctcgtttct ggaacaggtt gttcgtcaag ccttaatcaa
     5341 caacactatt gtaatagctg cccgaataga aagtgctgca ttgcctgata aaattgaaaa
     5401 agaaaagatt gcattaaaaa aacaattact ggttctctcc aggcaatttt taaattcctg
     5461 cagggaagaa ggcattcccg gggaaaaaat aatttttagc gtaccggcaa tcattgtgca
     5521 tgaactctct gaaaaaaaat tacctgaaaa aaaattaagt ttaacgctgt ttacccgatt
     5581 ctcacaggaa gatcattttg atcttgttga acttctcgcc a
//
LOCUS       DAOMYG010000191         5601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4963_length_5601_cov_8.174540, whole genome shotgun sequence.
ACCESSION   DAOMYG010000191 DAOMYG010000000
VERSION     DAOMYG010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4963_length_5601_cov_8.174540"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /gene="ccsA"
                     /locus_tag="PK610_07330"
     CDS             complement(<1..1115)
                     /gene="ccsA"
                     /locus_tag="PK610_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRJ35783.1"
                     /translation="MIETYTGEHLLPGLIGRFFVSLTFVASLIAFYTYLRASKATGEE
                     QDKWKKWGRCTFLIAAASSFIFIGTMFYLLVVQYFEYDYVWEQSNTKMSFEYILVCFW
                     GGQQGSFMLWIFWHSVIGLFLLRFAKGWENEVLAVFALLLAFISSILLGIYIGDYRVG
                     TSPFALQRETIDGLSALWSLIPDYLLLDPRFADGRGLNPLLQNYWMIIHPPTLFLGFA
                     LTIVPFAYAAAALWRRSYSEWIKPALPWTFAGILVLGIGILMGGAWAYESLSFGGFWA
                     WDPVENASLVPWITLVGAGHLMLVNRNQSRSLYSTFFLTLISFILILYSTFLTRSGVL
                     GDTSVHSFTGDGMLGQLLFCLLFFIWASFTLLLVDKKA"
     gene            complement(1112..1483)
                     /locus_tag="PK610_07335"
     CDS             complement(1112..1483)
                     /locus_tag="PK610_07335"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HRJ35784.1"
                     /translation="MKKTHIIALLVIAVSIGVILTSLSGASTYANFTQAFEQPGKKFT
                     VVGHLDHEYEITSEPMRCSFYIRDKENNLKKVVYNKAKPQDFERSESVVLTGSADGDT
                     FFASEISMKCPSKYNDVNKTQ"
     gene            complement(1480..1689)
                     /locus_tag="PK610_07340"
     CDS             complement(1480..1689)
                     /locus_tag="PK610_07340"
                     /inference="COORDINATES: protein motif:HMM:NF040113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HRJ35785.1"
                     /translation="MKTLLFLILALLYSYDSFAGGIETSMHAAGKMNVTLGVIAIIFI
                     GIVVYLFLIDRKITRLEKEIKERQS"
     gene            complement(1686..2687)
                     /gene="ccsA"
                     /locus_tag="PK610_07345"
     CDS             complement(1686..2687)
                     /gene="ccsA"
                     /locus_tag="PK610_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRJ35786.1"
                     /translation="MALLLYVIVVSLNTPLGPGLTAVSPENLNTGENSEIKVIGYNTH
                     FRNNPPDMQHPGVWLESDGFHLTCNNVEVIDDQELHFSVEVPDELPSKSLHVFVSYDE
                     GTFMLPNAFRAEQSVKNDQLKNEAPESFSGSVTAFYFPNREILRESIRNLMFHVPMWF
                     TMMLLMTISVVYSIKYLSGLNPDHDLIARQAVNVGLLFGILGLLTGSLWARFTWGQWW
                     VSDTKLNGAAITTLIYFAYTILRGSINEEQNKARVAAVYNVFAYVILIVLLMVLPRLT
                     DSLHPGNGGNPAFSSYDLDNALRTVFYPAVLGWMGISLWILQLRNRTERIKQQIENA"
     gene            complement(2733..3401)
                     /locus_tag="PK610_07350"
     CDS             complement(2733..3401)
                     /locus_tag="PK610_07350"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HRJ35787.1"
                     /translation="MLIREIGVLIRKEFDLEIRNKVAIGGILLYVVTTVFLCYLSFRE
                     LQDTTVWNTLFWVILLFSALNTISKSFQTESRYRLLYLYTLVSPAAVILSKMIYNILL
                     MGITALVTLLFYSLFLGTSPLEGANAGMYLVALLLGCAGFAGIFTMIAAIAAKTGNQL
                     GIMAILGFPLILPLLLTLIGLSGIALRGLPWEYGTTELLILTGLNILIAALSYILFPY
                     LWRE"
     gene            3452..4186
                     /locus_tag="PK610_07355"
     CDS             3452..4186
                     /locus_tag="PK610_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35788.1"
                     /translation="MRKLLLILLMPLSIPVFSQNPEVVLTTGHTDQVNTVDFSKDGRW
                     LATGGNDNNIKLWDVRTAREYRTLAGSDGRIVDVHFTKDSRKIGALNYNEQLRVWECK
                     SGKELFVIDGISSTSESFQFVRNDSLVFFVTNNHEFALADANSGKILIKKKMEWMPMR
                     MVASPDGKWIYALDHTGKLIELSMPDFSEHRSTQLFTTFQYSPARLMVDPTGKWLAAA
                     YDDQTVRIYATAELKEKKVLMGHQSR"
     gene            4187..>5601
                     /locus_tag="PK610_07360"
     CDS             4187..>5601
                     /locus_tag="PK610_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRJ35789.1"
                     /translation="MDLCFSSDGKRLFSVDHFGFIRSWDPESGKQLFAMEKKYVSIRR
                     MCMHPFENILAYTAFKDVFYMEAVTGKDLKVFRSKANRVLSMSYDQKGKFLATAYDDL
                     SIKLWDLEKLRITKQLDGFFPVCFNSSGNRLICMSNQIRLKVYDAENGTSIKDLDTDS
                     ELIQNLSCSNNDRFVAGAGFLGVIKIWDLESGKMVRKLNGHVNGVYATAWSPDDRFLV
                     SCGMDQTVRVWDMEKGNEIATLSGHEVLVHDVAFSPDGKYFASVAWDKTVRIWETGSW
                     KLWKELKGHINIVTAVTFSGDSKYVISGGGNNVVAPADNSIRVWDVSTGSEVCVFRNP
                     TGQINHLEAERKGNLLFSCSNDGMVKVWDLKKCTEIGSLISANKTDHIIVTPDHYYTA
                     SRGAFASVSFRIDDQLFPFEQFDMRLNRPDIVASRIGKTPANLVNAFNYVYRKRLERL
                     NFREEDFGTKFQVPEVFLDRSA"
BASE COUNT         1727 a         1123 c         1259 g         1492 t
ORIGIN      
        1 gcttttttat caaccaaaag caaggtgaat gatgcccaga tgaaaaacaa caaacaaaac
       61 aaaagctgac ctaacattcc atcgccggta aaggagtgga cagaagtatc acccaagact
      121 ccgcttcggg taagaaacgt tgaatagagg atcaaaataa agctgatcag tgtaaggaag
      181 aaagtcgaat aaagtgagcg tgactgatta cggttgacaa gcattaaatg tccggcccca
      241 accaaggtga tccatggaac caacgatgca ttctcaacag ggtcccaagc ccaaaatcct
      301 ccgaaactca gcgattcata cgcccatgcc cctcccatca aaataccaat gcctaacaca
      361 agtattccgg caaatgtcca gggaagtgca ggtttgatcc attcggaata agaccgtcgc
      421 cataatgcgg ctgctgcata ggcaaatggc acaatagtca atgcaaagcc gaggaaaagc
      481 gttgggggat gaatgatcat ccagtaattc tgaagcaagg gattcaatcc gcgtccatct
      541 gcaaagcgag gatcgagcaa tagataatcg gggattaagg accaaagcgc gctgagccca
      601 tcaatggttt cgcgttgtaa ggcaaaagga cttgtcccaa cccgataatc gccgatataa
      661 ataccgagca aaatactcga aataaaagcc agcaacaatg cgaatactgc gagtacttcg
      721 ttttcccatc cctttgcaaa acgcagtaaa aacaatccta taactgaatg ccagaaaatc
      781 caaagcataa aacttccctg ctgtccgccc caaaagcaaa ccaggatgta ttcgaacgac
      841 atctttgtat tgctttgttc ccacacgtaa tcgtattcga aatactgcac cacgagcaga
      901 taaaacattg taccaatgaa aataaatgaa gatgctgcag caatcagaaa agtacagcgt
      961 ccccattttt tccatttgtc ctgttcttct ccggtagctt tactggcacg cagataagtg
     1021 taaaaagcaa taagggaagc aacgaaggta agcgaaacga aaaatcgtcc gattaatccg
     1081 ggcaggaggt gttctccggt gtaggtttcg atcactgtgt tttattaacg tcgttatact
     1141 tcgaaggaca tttcattgaa atctcacttg caaaaaaggt atcaccatcg gcagaacctg
     1201 tgagtaccac gctttcagaa cgttcaaaat cctgtggttt ggctttgttg tagaccactt
     1261 ttttcaggtt gttttcttta tcccgaatat aaaacgaaca acgcatgggt tcagatgtga
     1321 tttcgtattc gtgatccaga tgaccaacca ctgtgaattt ttttccgggt tgctcaaagg
     1381 cctgcgtaaa gttggcatat gtactagcac cgcttaatga agtaagtatt actccaatgg
     1441 atacagcgat taccaataaa gctatgatgt gggttttttt catgactgac gttctttaat
     1501 ttctttttca agacgcgtaa tcttacgatc gatgagaaaa agataaacta ctattccaat
     1561 gaagatgatt gcaattacac caagtgtaac attcattttt cctgcggcgt gcatagaggt
     1621 ttctattccg ccggcaaaag aatcataact gtatagcaat gccagaatta gaaaaagtag
     1681 agttttcatg cgttttcaat ttgttgtttg attcgttctg tcctgttgcg aagctgaaga
     1741 atccataatg atattcccat ccatcctaaa acagccggat agaacaccgt tcgcaatgcg
     1801 ttatccaaat cgtaactgct gaatgcggga ttgccaccgt ttccgggatg taaagagtcg
     1861 gtaagtcggg gaagcaccat gagaagtacg atcagtatga cgtaagcaaa cacattgtaa
     1921 acagctgcca cccttgcttt gttttgttcc tcgttgatcg atccgcgaag gatggtgtag
     1981 gcaaagtaaa tcagtgtggt aatggcagca ccgttcagtt tggtatcgct cacccaccat
     2041 tgaccccaag taaatcgggc ccacaaagaa ccggtgagca ggccaagtat tccgaaaagc
     2101 aatcccacat ttactgcctg ccttgcgatt aaatcgtgat caggatttag tccggaaaga
     2161 tatttgatgc tgtaaacaac tgaaattgtc atgagcaaca tcatggtaaa ccacatggga
     2221 acatgaaaca tcagattgcg aatactctca cgcaaaattt cgcgattggg aaagtagaat
     2281 gcagttactg atcctgaaaa actttccggt gcttcatttt tgagttgatc gtttttaaca
     2341 ctttgctcag cacgaaaagc gttgggaagc atgaaagtgc cttcatcata gctcacgaaa
     2401 acatgcaggg attttgacgg taattcatcg ggaacctcca ccgaaaaatg cagttcctgg
     2461 tcgtcaatta cttccacatt attgcaggtc aggtgaaatc catccgattc gagccaaact
     2521 ccgggatgtt gcatatccgg tggattgttg cggaaatggg tgttgtatcc tattactttg
     2581 atttcggaat tttctccggt attcaggttt tccggtgaaa cagcagttaa acccggacca
     2641 agtggggtgt ttaggctcac cacaatgaca tacagcagta gagccacact taaatatttc
     2701 caccagtttt tcattcggag agctgcttca gattattcgc gccaaaggta aggaaataag
     2761 atataggaga gggctgcaat gaggatgtta agacctgtta aaatgagcaa ttcggtggtt
     2821 ccgtactccc agggtaatcc cctgagcgct atgccagaaa gtccaattaa tgtaagcaat
     2881 aaaggcagaa tcagaggaaa gccaagaatg gccattattc ccaattggtt acctgttttc
     2941 gctgcaatag ctgcaatcat ggtgaaaata cccgcaaaac ctgcacagcc gagaaggagt
     3001 gcaaccaggt acatacctgc attcgcgcct tcgagtggac ttgtccccag aaataaggaa
     3061 taaaagagta gggtgaccaa agctgtaata cccatgagca ggatattata gatcatttta
     3121 ctcaaaatta cagcggcggg cgaaacgagg gtgtaaagat agaggagtcg gtaacggctt
     3181 tcggtttgga aacttttgga gatggtattc agggcagaaa agagcaggat tacccaaaat
     3241 aatgtattcc aaacagtagt atcctgcaat tcgcggaacg agaggtagca taaaaaaaca
     3301 gtggtcacca catacagcag aatacctcca attgccactt tgttgcggat ttcaaggtca
     3361 aattcctttc gtatgagcac accgatttcg cggattaaca tgttgctaag gtaatgaaaa
     3421 cagttgcacg gcttttgcta cctttatcac aatgcgaaaa cttcttctaa ttcttctgat
     3481 gccattgagc atcccggttt ttagtcaaaa tcccgaagtt gtactcacca cgggtcatac
     3541 cgatcaggtc aataccgtcg atttcagcaa agacggtcga tggcttgcta caggggggaa
     3601 tgataacaat atcaaattat gggacgtccg cacagcccgt gaataccgta cacttgctgg
     3661 gagcgacgga cgaattgttg atgttcattt cacaaaagat tcgcgcaaaa ttggagcgct
     3721 taattataat gaacagctga gagtttggga gtgcaaatca ggaaaagaac tttttgtaat
     3781 agatggaatt agctcaacct ctgagtcctt tcagtttgtg agaaatgatt cattggtatt
     3841 ttttgtaaca aacaaccatg aatttgcgct ggccgatgcc aattccggca aaattttgat
     3901 caaaaagaaa atggaatgga tgccaatgcg gatggtagca tcgcccgatg gtaagtggat
     3961 atatgcgctg gatcataccg gtaaactcat tgaactttca atgcctgatt tttctgaaca
     4021 cagatcaact caattattca caacatttca atattcaccg gcacgtttaa tggttgatcc
     4081 tacagggaaa tggttagctg ctgcatatga cgatcagact gtaagaatat atgcaactgc
     4141 tgaattaaag gaaaaaaaag tgctaatggg acatcaatca cgataggtgg atctctgttt
     4201 ttcatccgat gggaaacgtt tgttttcagt tgatcatttt ggatttatca gatcctggga
     4261 tcctgaaagt ggaaaacaat tgtttgcaat ggagaaaaaa tatgtgtcga tccgcagaat
     4321 gtgtatgcat ccattcgaga atattttggc ctacactgca tttaaggatg tattttatat
     4381 ggaagccgtc acaggtaagg atctcaaagt ctttcgttca aaggcaaaca gggtgttgag
     4441 tatgagttat gaccagaaag ggaaatttct agctactgcc tacgatgatt taagcattaa
     4501 gctatgggat ctggaaaaat tgcgcatcac caaacaactt gacggattct ttccggtatg
     4561 tttcaattct tccggaaacc gattgatctg tatgagtaat cagatccgat taaaagtgta
     4621 tgatgctgaa aatggcacaa gcattaaaga ccttgatact gatagtgaac tgattcaaaa
     4681 tctctcatgt agtaacaacg accgctttgt tgccggtgca ggttttttgg gtgtaataaa
     4741 aatctgggat ctggaatccg gaaaaatggt gcgtaaactc aatgggcatg taaacggagt
     4801 atatgctact gcatggagtc ccgacgaccg ctttctagtt tcgtgcggaa tggatcagac
     4861 tgttcgcgtt tgggacatgg aaaaagggaa tgagattgca actttaagcg gacatgaagt
     4921 attagtgcat gatgttgcgt ttagtccaga tggaaaatat tttgcttctg tggcttggga
     4981 caaaaccgtt cggatttggg aaactggatc ctggaaactc tggaaagaat tgaaagggca
     5041 tatcaatatt gtaaccgcag ttacttttag cggcgatagc aaatatgtta tttcaggagg
     5101 aggtaataat gttgttgcac cagcagataa cagcattcga gtttgggatg tttccaccgg
     5161 aagtgaagtg tgcgtttttc gcaatccaac aggacagatt aatcaccttg aagctgaacg
     5221 aaaggggaat ttattattct cctgttccaa cgatgggatg gtgaaagtgt gggatctgaa
     5281 aaaatgcaca gagatcgggt ctctaatttc tgcaaataaa acagatcaca ttattgttac
     5341 tcctgatcat tattataccg catccagagg tgcctttgca tctgtttcct ttaggattga
     5401 tgatcagttg ttcccatttg agcaatttga tatgcgatta aatcgtccag atatagtggc
     5461 atcgcgaatc gggaaaacac cagcgaatct ggttaatgca tttaactacg tatatagaaa
     5521 aaggctagaa cgattgaatt tcagagaaga agatttcggg acaaaatttc aggtacccga
     5581 ggtgtttctt gatcgatccg c
//
LOCUS       DAOMYG010000192         5572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_4986_length_5572_cov_8.512416, whole genome shotgun sequence.
ACCESSION   DAOMYG010000192 DAOMYG010000000
VERSION     DAOMYG010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4986_length_5572_cov_8.512416"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /gene="ispE"
                     /locus_tag="PK610_07365"
     CDS             <1..726
                     /gene="ispE"
                     /locus_tag="PK610_07365"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRJ35790.1"
                     /translation="PVSWRDILEAIPNETGLFSIEFSGLPIPGDPKDNLIYKAWKLLN
                     EKHGIPGVNVHLHKVIPMGAGIGGGSADGAFMLKLLNSLFALDLSERELMEMASSLGS
                     DCPFFIPNTPAFVTGRGEKMEQLLFNLDDYRLVLINPGLHFGTAEAYSGIVPNESGIS
                     TKEVIEKYPVAEWKNYLKNDFEIPAFKKYPELRHLKDALYANGALYASMTGSGSTLYA
                     LFHSIPENLGNCICKEGYTIKIC"
     gene            complement(735..1802)
                     /locus_tag="PK610_07370"
     CDS             complement(735..1802)
                     /locus_tag="PK610_07370"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRJ35791.1"
                     /translation="MITRIDRYIMGKFLGTFFFILAILMAIAIVFDFAEKVDDFIEFK
                     VSFWDLLTKYYINFVFHYSNLFSSLIIFISVIFFTSKMAKNNELIAVLCSGMSFNRML
                     RPFFLAATVLVVLSIFLNHLVLPRANKIRLDFEESIARHSVLLNNVHLEVEPGVIVYF
                     DNNHYGYLDAFCMEKWEEGSLKSVLTAQRAYYDTLNTKWSLQFYHIRYVSEYKDSIVR
                     GDKRDTTFNFAPSDLGYRNEFSSSMTTPELLRFIETEKRRGSTKVQLSQIELHQRTSY
                     PAAAYILTLIGVCVAGRKSRGGIGLNIAIGLVLAVTYIFSMRLTSVAATNAGLDASIA
                     VWIPNFMFLLLTFPLYRFAQK"
     gene            complement(1802..2932)
                     /gene="tgt"
                     /locus_tag="PK610_07375"
     CDS             complement(1802..2932)
                     /gene="tgt"
                     /locus_tag="PK610_07375"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRJ35792.1"
                     /translation="MQFQLKANDPGSSARAGIITTAHGEIPTPIFMPVGTQSTVKAVH
                     QRELKEDIKAKIILGNTYHLYLRPGTELLKSAGGLHKFMNWDGPILTDSGGYQVYSLS
                     ENRKIKEDGVTFRSHIDGSKHLFSPEYVMDVQRIIGADIIMAFDECTPYPCDREYATR
                     SMEMTHRWLKRCVDRFDATEPLYGYEQTLFPIVQGSTYDDLRKKSAETIASFNRPGNA
                     IGGLSVGEPEEEMYRHTEIVCNILPADKPRYLMGVGTPVNILENIALGVDMFDCVMPT
                     RNARNGMLFTSEGIINIRNERWKDDLSVLDPNGTTWVDQSYSKAYLRHLTVAGEMLAA
                     QIASIHNLGFYLWLVAEARKHILEGDFVSWKNHMVPKLKNRL"
     gene            3005..4120
                     /locus_tag="PK610_07380"
     CDS             3005..4120
                     /locus_tag="PK610_07380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ35793.1"
                     /translation="MKFGPEELLLIFFCAAAFVQLFYYFFFYFRIAFHKERHHNTLTP
                     PVSIIICARSESSKLVHNLPHIFDQDYPDFEVIVVNDRSWDDTKEILKAFQIRYPHLR
                     VINIEESSHEHYGKKMALTIGIKGAKNEWLLLTDADCKPLHRNWIKKMVEAQSANTEF
                     VLGYSPYQNKKGFLSKLIRFDTLLAGMNYLSFAKSGLPYMGVGRNLMYKKESFFAVRG
                     FKKHYHISSGDDDLFINQAANGKKTSVMFHPESHVVSMPKTSFVDWFRQKKRHFTTAP
                     HYKLRDKILLSLFPASYYLGIGLSIPLLVLNTYLWIILGIIGFRLLLQIVIFSRSMKW
                     LGDKDLLLWAPVLEFFVLILHPVIHLSNKIVKATKWN"
     gene            4111..4725
                     /locus_tag="PK610_07385"
     CDS             4111..4725
                     /locus_tag="PK610_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ35794.1"
                     /translation="MELEEGFENLSDKARYDFQLVIRAREGEQAAYAELMERYRDSIY
                     FMLLKMVNNKDDADDLTIEAFGKAFNRLDLYTPNFAFSTWLFKIASNNCIDFIRKKKK
                     NTLSIDSAFAGEDGDEMYIDLRSDALTPEEEAIKQQKEKLMRDVVQKLKPRYRQLIEM
                     RYFQQLAYEEIAEQLDLPLGTVKAQLFRARDLLYQVLKNSKDRF"
     gene            complement(5065..>5572)
                     /locus_tag="PK610_07390"
     CDS             complement(5065..>5572)
                     /locus_tag="PK610_07390"
                     /inference="COORDINATES: protein motif:HMM:NF020922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HRJ35795.1"
                     /translation="VKMEAKDAIFRINRDIRFSKDKYPYKMHMAAAVSRLGKKEPDVC
                     GFYFQLNPEKIQIWHGAYFIETPTLHQLRNYIAANLKTFTKLQRSKKFVDYYGEVVGD
                     GNSRLSKELKAAAEQEPLLFHKNLYWGAELPAKMITSKDLLKTIIEHYKAGKAMADFI
                     DEGMKGKQ"
BASE COUNT         1638 a         1225 c         1128 g         1581 t
ORIGIN      
        1 cctgttagct ggagagatat tttggaagcg attcccaatg aaaccggatt gttttccatt
       61 gaattttccg gtttgcccat acccggagat ccaaaagaca atctgattta caaagcctgg
      121 aaattactta atgaaaaaca tggtattccc ggagtgaatg tgcatttgca caaagtaatc
      181 ccaatgggag ccggaatagg tggtggaagt gctgatggtg catttatgtt gaaactattg
      241 aattcacttt tcgcactgga tctttcagaa cgggaactga tggaaatggc atcgagtctg
      301 ggaagtgatt gtccgttttt cataccgaat actccggctt ttgtaaccgg cagaggagaa
      361 aaaatggaac aacttctatt caatctggac gactatcgac tggtattgat aaatccagga
      421 ttgcactttg gtactgctga agcttatagt ggaattgtgc ccaatgaatc agggatcagc
      481 acaaaggaag ttatcgaaaa atatcctgta gctgagtgga aaaactatct gaaaaatgat
      541 tttgaaattc cggcttttaa aaaatatcct gaactccgcc atttaaaaga cgcactctat
      601 gcaaatggag cgctctatgc atccatgacc ggatcaggaa gtacgcttta tgcattgttc
      661 cattccattc cggaaaatct cgggaactgt atctgtaaag aaggctatac aattaaaatc
      721 tgctgatcga tttcttattt ctgagcaaat ctgtaaagcg gaaaggttag caaaagaaac
      781 atgaaattcg gaatccaaac tgcaatggat gcatccaaac ctgcgttggt tgctgcaaca
      841 gaggttaacc gcattgagaa aatataggtt actgccagca ccagtcctat cgcaatattc
      901 aatcctatac cacctctcga ttttcgtccc gccacacaaa caccaatgag tgttagaata
      961 tatgcagctg caggatagga tgtccgctgg tgaagttcga tttggctcag ctgaactttt
     1021 gtacttcccc tccgtttttc tgtttcgatg aaacgcaata attcgggagt agtcattgaa
     1081 cttgaaaatt catttctgta tccaagatca gaaggcgcga aattgaatgt cgtatcgcgt
     1141 ttatcgcctc ttacaattga atctttgtat tctgaaacgt aacggatatg gtagaattgc
     1201 agcgaccatt tcgtattcag ggtatcataa taagctctct gggcagtcag cacacttttg
     1261 agtgatcctt cttcccattt ttccatacaa aaagcgtcga ggtaaccgta gtggttgtta
     1321 tcaaaataaa caataacacc cggctctact tcgaggtgga cattgttgag caaaacggaa
     1381 tgtcgtgcaa tgctctcttc aaaatcaagt cggattttat tcgcccgcgg caggaccagg
     1441 tgattcagaa agatagacaa aacaacaagc acagtagccg caagaaaaaa gggacgcagc
     1501 atacggttaa agctcattcc actgcacaac actgcaatca attcattgtt ctttgccatt
     1561 ttggaagtaa aaaaaatgac agaaataaaa atgatgagcg aactgaaaag attggaataa
     1621 tgaaaaacga aattgatgta atattttgtg agaagatccc agaacgaaac tttgaattca
     1681 ataaaatcat ccactttttc agcgaaatca aatacgatcg caatcgccat caaaattgcc
     1741 aggatgaaga aaaatgtccc caggaatttc cccatgatat acctgtcaat tcgggtaatc
     1801 atcagagtcg gtttttaagt ttaggcacca tatgattttt ccaggaaaca aaatcgcctt
     1861 ccagaatatg ctttcgcgct tcggcaacca gccagaggta aaaaccaaga ttatgtatgg
     1921 atgcaatttg tgctgccagc atttcacccg ccacagtaag atgacgcaga taggctttgc
     1981 tgtaggactg atccacccat gtagttccgt ttggatcgag cacggagaga tcatctttcc
     2041 acctttcgtt gcgaatattt attattcctt cagaggtaaa caacattccg tttcgcgcat
     2101 tgcgggtagg cataacacaa tcgaacatat ccacacccaa agcaatattt tcaagaatat
     2161 ttaccggtgt acccacgccc ataaggtaac gtggtttatc ggctggtagt atgttgcaga
     2221 caatctcggt atgccgatac atttcctctt cgggttcacc aaccgacaat cccccaatgg
     2281 cattacccgg ccggttaaag gatgcgatag tttctgccga cttttttcgc agatcatcgt
     2341 atgtactccc ctgcacaata ggaaacaagg tttgttcata gccgtacaaa ggctccgtgg
     2401 catcaaagcg atcgacacaa cgtttcagcc agcggtgtgt catttccatg ctgcgtgttg
     2461 catactcccg atcgcatgga tagggtgtgc actcatcaaa agccatgatt atatcagccc
     2521 cgatgatgcg ctggacatcc attacatatt ccggagaaaa aaggtgtttg gatccatcaa
     2581 tatgcgaacg gaatgtaaca ccgtcctctt tgatttttcg gttttcgctg agcgaataca
     2641 cctgatatcc tcccgaatcg gttagaatcg gaccatccca attcatgaat ttatgaagtc
     2701 caccggcact tttcaacagc tcggtgcccg gccgcaggta gaggtggtag gtattaccaa
     2761 ggattatttt tgccttaata tcctccttta attcacgctg atgcacagct ttaacagtag
     2821 actgggtgcc aaccggcatg aaaataggag ttggaatttc gccatgcgca gtggtaatta
     2881 tacctgccct tgccgaggaa cccggatcgt ttgcctttaa ctgaaattgc atctgttgaa
     2941 aaaaagaggt gggtcaaaga taacaactta cagcgttctg tacttttgcc ccccacgact
     3001 tatcatgaaa ttcggacccg aagaactatt actgatcttt ttttgtgcag cggcatttgt
     3061 gcagttgttc tactacttct ttttctattt ccgaattgct tttcacaagg agaggcatca
     3121 caataccctt accccacccg tttcgatcat catttgcgcc cgatctgaat cgtcgaaact
     3181 cgtacataac ctccctcata ttttcgatca ggattatccc gatttcgaag tgattgttgt
     3241 taacgacaga tcctgggacg ataccaagga aatcctgaaa gcattccaga tccgttatcc
     3301 gcatttgcgt gtgattaata ttgaagaatc gagccacgaa cattacggta aaaaaatggc
     3361 ccttaccatt ggtatcaagg gcgcaaaaaa tgaatggttg ttattgaccg atgccgactg
     3421 caaacccctt caccgcaact ggataaaaaa aatggtggaa gcacaatctg ccaacaccga
     3481 atttgtactt ggttacagtc cctaccaaaa caaaaaagga ttcttaagca agctaattcg
     3541 ctttgacacc cttttagcag gaatgaacta tttatccttt gctaaatctg gtctgccata
     3601 catgggtgtt ggacgaaacc ttatgtacaa gaaggaatcc ttttttgccg tacgtggctt
     3661 taagaaacac tatcacatct catccggaga tgatgacctg ttcatcaatc aggctgcaaa
     3721 cggaaagaaa acatccgtaa tgtttcatcc cgagtcgcac gttgtttcga tgcccaagac
     3781 aagttttgtg gattggttcc ggcaaaaaaa gcgtcatttt accactgcac cccattacaa
     3841 gcttcgcgac aaaattcttc taagcctctt ccctgcaagc tattacctcg gaattggcct
     3901 cagcattcca ctacttgttt taaacacgta tctatggatt attttaggca tcatcggatt
     3961 caggctgctg cttcaaatag ttatctttag ccgttcaatg aaatggcttg gcgacaaaga
     4021 ccttttgttg tgggctccgg tgttagagtt ttttgttttg attcttcatc ctgtcattca
     4081 tttgagtaat aaaattgtga aagcaacgaa atggaattag aagaaggttt cgagaatctg
     4141 tccgacaaag cacggtacga cttccagctt gtcattcgcg ctcgcgaggg agaacaagct
     4201 gcctatgccg aattgatgga aaggtaccgc gactcgattt atttcatgtt gttgaagatg
     4261 gtcaacaata aagacgatgc cgacgatctc accattgaag cttttggtaa agcctttaac
     4321 cgtttagacc tttacactcc gaattttgct ttctccacct ggttgttcaa gattgcatcc
     4381 aacaattgta ttgactttat ccgcaagaag aaaaagaata ccctatccat cgacagtgca
     4441 tttgcaggag aagatggaga cgagatgtat atcgatcttc gttcggacgc actcactccg
     4501 gaagaggaag ccataaaaca acaaaaggaa aagctgatgc gcgatgttgt gcaaaagcta
     4561 aagccacgat atcgccaatt gattgaaatg cgttatttcc aacaattggc ctatgaggaa
     4621 atcgcagaac aactcgatct tccattggga accgtaaaag ctcaattgtt cagagcgcgt
     4681 gatcttttgt accaggtatt gaaaaactcc aaagaccgtt tttaaaagaa tccggttatt
     4741 gaaccacact tatcactttt caagctaaac tacccaccct tctccgaaat tgtatttgaa
     4801 agaaaccgca aagagaaaga gaggtgaaga attgaattta tttgcaccta ctccaatcga
     4861 tgattcatat tcatctgaaa tttccgatgc aggaaatatg attagtttaa aacggaatcg
     4921 agccacctac agtgtctacc aaaggttgta ccactgaaat tcccgcggta ctgaaggcgt
     4981 aggtagaatg agataaccaa ttgctgtatc acccctcact gttcaccgtt cactgtttac
     5041 ccttcactgt ttaccgtgca ctgttcactg tttacccttc attccttcat caataaaatc
     5101 agccatggct ttacctgcct tgtagtgttc gattatcgtt ttgagtaaat cttttgaggt
     5161 aatcatttta gcaggtagtt ctgctcccca gtaaagattc ttatggaaca acagtggctc
     5221 ttgttcagct gcagctttca attccttcga aagacgcgaa tttccgtcgc ccacaacttc
     5281 tccgtagtaa tccacaaact ttttcgaccg ttgtaatttg gtaaatgttt tgagattggc
     5341 agcaatataa ttccttagtt gatgcaaggt gggagtttca ataaaataag ctccatgcca
     5401 aatctgaatt ttctccggat ttaactgaaa ataaaatccg caaacatcag gctctttttt
     5461 ccctaatcga gaaacagctg ctgccatatg cattttgtaa gggtatttat cttttgaaaa
     5521 ccgaatatca cgattgatcc tgaagatggc atctttagct tccattttca ct
//
LOCUS       DAOMYG010000193         5550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5013_length_5550_cov_11.709372, whole genome shotgun sequence.
ACCESSION   DAOMYG010000193 DAOMYG010000000
VERSION     DAOMYG010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5013_length_5550_cov_11.709372"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PK610_07395"
     CDS             <1..817
                     /locus_tag="PK610_07395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ35796.1"
                     /translation="KRIPLVYDTHEYFTEVPELVNRPRVRRIWERIEERIFPKLEHII
                     TVNESIADMYHKKYGKTIHVVRNIPSIKTGNEKKSRQELHLPEDKTILIIQGAGINID
                     RGNEEMVMAMEHLSNCLLLIIGNGDVVEDLKKLSQQLQLQDRILFFPRMEYSELMQYT
                     RNSDIGITLDKDSNLNYKFSLPNKLFDFIHAGIPVLASDLPEVKRITLGYKTGHIVSS
                     HDPKTIAAAIKKMMDDPKEMEAMRNNTKTAALDLTWEKECETLNKIYRALGAV"
     gene            complement(831..1181)
                     /gene="crcB"
                     /locus_tag="PK610_07400"
     CDS             complement(831..1181)
                     /gene="crcB"
                     /locus_tag="PK610_07400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRJ35797.1"
                     /translation="MNIVYVFLGGGLGSVMRYLLSLGVPQLVNMRFPAATFLANTIAC
                     IILVLVATWAKGKSADGTMLFLVTGFCGGLSTFSTFSFENVQLLQQGNYLVASANIVL
                     SLIAGIGVIYLMWK"
     gene            complement(1183..1752)
                     /locus_tag="PK610_07405"
     CDS             complement(1183..1752)
                     /locus_tag="PK610_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRJ35798.1"
                     /translation="MDNLELIDRSLFFLINGHHSPFFDQLMELVSMRLIWIPAYVFLA
                     WALYRFYGVNGLLIGLVSAGILIALTDAGSNYLFKHNVQRYRPCHNTEIAHLVHLVNN
                     HCGGMYGFISSHAANFFGLATFFSFLLKSHWRKSPFLFFFAAILVSYSRIYLGVHYPS
                     DIAVAAIWGILWGWISYLLFQNLYSKKIN"
     gene            complement(1752..2741)
                     /gene="obgE"
                     /locus_tag="PK610_07410"
     CDS             complement(1752..2741)
                     /gene="obgE"
                     /locus_tag="PK610_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRJ35799.1"
                     /translation="MDSNFVDYVKICGRSGKGGAGSAHFHRGKGNPTGGPDGGDGGRG
                     GHIILRGNAQLWTLIHLKYRKHVIAEEGQNGGSNRMTGRSGRDEVLDVPLGTVARDAE
                     TGEILFEITKEGEERILVPGGRGGLGNDHFKTSTNQAPNYAQPGEPGREEWKILELKV
                     LADVGLVGFPNAGKSTFLSVVSAAKPEIADYPFTTMVPNLGIVQYRDYRSFIVADIPG
                     IIEGAHLGKGLGHRFLRHIERNSILLFMVPADAADIKKEFKILENELKMFNPELLHKD
                     RILAITKCDMLDEQMMEEMKQELPKGVETVFISSVTGFNIQELKDKLWRKLNS"
     gene            complement(2804..3385)
                     /locus_tag="PK610_07415"
     CDS             complement(2804..3385)
                     /locus_tag="PK610_07415"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRJ35800.1"
                     /translation="MLNIVLFGPPGAGKGTQSENLIAKYKLVHLSTGDIFRANIKGGT
                     ELGNLAKSYIDKGQLVPDEVTIRLLESEVNKNPSAGGFIFDGFPRTSAQAEALDSFLD
                     SKGQSITVMLSLEVPEEELKRRLLERGKTSGRADDADPNVIQKRIDVYNAETTPVKAY
                     YQQQNKCSEIHGVGSIDEIFSRLSSAIDAAAVK"
     gene            complement(3400..3933)
                     /gene="hpt"
                     /locus_tag="PK610_07420"
     CDS             complement(3400..3933)
                     /gene="hpt"
                     /locus_tag="PK610_07420"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRJ35801.1"
                     /translation="MKVTLKDKTFALYLREEEILGNIAEIGKRISADYAGRTPLLLGV
                     LNGSFLFAADLIREVKCDCQITFVKLASYIGTGSSGEVRELVGLAEDIRGRDIIVVED
                     IVDTGLTLEKVMDTLQKHSPASVKVATLLYKPEAYKGKHKIDYVAMEIPNAFIVGYGL
                     DYDGLGRNLRDIYQIVS"
     gene            complement(4017..4532)
                     /locus_tag="PK610_07425"
     CDS             complement(4017..4532)
                     /locus_tag="PK610_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRJ35802.1"
                     /translation="MKTIEFLHPYEYQMLETLVVVLVVIVLRAIVTRVFRRVTTAIEI
                     EHRRAKMLGRIVRVFINVLGVIILLMIWGVDQRELFLFVSSMLTLLGIAFFAQWSLLS
                     NITSAVLLFINHPAKVGSKIQIMDKDYPVTGTISEIGVFFITIKTIEGTTVTIPNAVF
                     IQRMISLLPEE"
     gene            complement(4547..>5550)
                     /locus_tag="PK610_07430"
     CDS             complement(4547..>5550)
                     /locus_tag="PK610_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRJ35803.1"
                     /translation="IKRFSILTKLTLIAVLLVVLAGSVVRMSGSGMGCPDWPRCFGQW
                     IPPTDISQLPADYKEVYAAKRAKKLEDFCRLLDRTGFSQEATAMRSDSSLLEEQDFNA
                     TKTWTEYINRLIGFVSGNLMLVQFILSLWWWKTDKRLIFFCLLNLILLAVTAWFGAIV
                     VATNLLPWVLTVHMVLALLIIVLQVVIIERASMQKEKIQVPKTIRVLLGLVIVIGLLQ
                     ILMGTQIRQQIDALYAQFGGEDRHLWIDQLTTIFYIHRSFSWMIVLLTIFIWYKLRES
                     SVELPGIKFLFSVIAIEVVSGATLAYFNMPALIQPLHLLLACMMIIVQMRMFLQTQS"
BASE COUNT         1716 a         1314 c         1125 g         1395 t
ORIGIN      
        1 aaaacgaatt cctctggtgt acgataccca cgaatatttt acggaagtcc ccgaactggt
       61 aaatcgtccg cgtgtccgcc gcatctggga gcggattgaa gaacgtatat tcccaaaact
      121 ggaacacatc ataaccgtaa acgaaagcat tgcggatatg taccacaaaa aatacgggaa
      181 aacgatccat gtcgtgcgca acattccatc catcaaaacg ggaaatgaaa aaaaaagcag
      241 gcaggaacta cacctacccg aagacaaaac cattctgatc atacagggag caggcattaa
      301 catcgaccgt ggcaatgaag aaatggtaat ggcaatggaa cacctcagca actgtttgct
      361 gctcatcatt ggcaatggag atgttgtgga ggacctgaaa aaattatcgc aacaattgca
      421 attacaggat cgcattctgt tttttccgcg gatggaatac agcgaattga tgcaatacac
      481 ccgcaacagt gatattggaa ttaccctcga taaagacagc aacctcaatt acaaattcag
      541 tctgcccaac aaactcttcg actttatcca tgcaggaatt cccgttttgg cttctgattt
      601 accggaagta aaacgcatca cattgggtta caaaaccgga catattgttt cttcacacga
      661 tcccaaaaca attgcagctg ccattaaaaa aatgatggat gatcccaaag aaatggaagc
      721 gatgcgcaac aacaccaaaa ccgcagcgct tgatcttacc tgggaaaaag aatgtgaaac
      781 actaaataaa atttaccgtg cgcttggtgc tgtttaaatc gccgcacaaa tcacttccac
      841 atcagataga tcacaccaat tcctgcaata agtgacagta caatattggc actagcaacc
      901 aggtaattcc cctgctgcaa caattgaaca ttctcaaaac taaacgtaga aaacgtactg
      961 agtccgccac aaaacccggt taccagaaac aacatggttc cgtctgccga tttacctttt
     1021 gcccaggttg caaccaacac cagaatgata caggcaatgg tattggcaag aaaagttgct
     1081 gcaggaaagc gcatgtttac caattgagga acacccagtg ataagagata acgcattaca
     1141 ctacccaatc ctccacccag aaaaacgtaa acgatgttca tctcagttta tttttttgga
     1201 atacagattt tgaaacaaca ggtaactgat ccatccccac aatatacccc aaattgcggc
     1261 aactgcaata tcggaaggat aatgtactcc cagataaatg cgcgaatacg aaaccagaat
     1321 tgcagcaaaa aagaacagga agggcgattt tctccaatgg gattttaaca aaaacgaaaa
     1381 gaaagtagcc aatccaaaaa aattggcagc atgcgaggaa ataaatccgt acatgcctcc
     1441 gcaatggttg tttaccagat gtacaaggtg agcaatttcg gtattgtgac agggacggta
     1501 acgctgcaca ttgtgtttga acagataatt agagcctgca tccgtaagcg caatcaaaat
     1561 accggcagat actaaaccaa tgagcaatcc atttactccg taaaaacggt acaatgccca
     1621 ggcaagaaaa acataggcag gaatccagat gagtcgcatc gaaaccagct ccatcaactg
     1681 atcgaaaaac ggagaatgat gtccgttgat cagaaaaaac aaagagcgat cgatcaactc
     1741 aagattgtcc atcagctgtt cagtttacgc cagagtttgt ctttgagttc ctgaatattg
     1801 aaacctgtta ccgatgaaat aaatacagtt tctactcctt tcggtaattc ctgtttcatt
     1861 tcttccatca tctgctcatc gagcatatcg catttggtaa ttgcaagtat gcggtcttta
     1921 tgcaataatt caggattgaa cattttcagt tcgttctcca gaattttgaa ttccttttta
     1981 atatcagcag catcggcagg aaccatgaag agcaatatgg aattgcgttc gatgtgacgc
     2041 aggaaacggt gtccaagtcc tttgcccaga tgcgcacctt caataattcc gggaatatcg
     2101 gcaacaatga aagagcggta atcgcggtac tgcacaatac caagattggg aaccatggta
     2161 gtaaagggat aatcggcgat ctccggtttt gcagcggata ctacagagag aaaagtcgat
     2221 ttccctgcgt tgggaaatcc taccagtcca acatcggcaa gtactttcaa ctcaagaatt
     2281 ttccattcct cgcgaccggg ttctccgggt tgtgcgtagt tgggagcctg attggttgat
     2341 gttttaaaat ggtcgttccc caaaccaccg cgaccgccgg gaacgaggat ccgttcttcg
     2401 ccttccttgg tgatctcaaa caatatctct cccgtttccg catcacgcgc aacagttcca
     2461 agcggaacat ccaatacttc atcgcgacca cttcttcctg tcattcggtt ggatcctccg
     2521 ttctgacctt cttctgcaat tacatgctta cggtatttca ggtgaatgag tgtccataac
     2581 tgtgcattgc cacgaagaat aatatgacca ccgcgaccac catcaccacc gtcaggtcct
     2641 cctgtgggat ttccttttcc gcggtggaaa tgcgcagaac ctgctccgcc ttttccggaa
     2701 cgaccgcaga tttttacgta gtcaacaaaa ttcgaatcca tgttcttttt ctaaacaaca
     2761 aaacctgtcc gcagacaggt ccggttggtg tgtttgcgat taattatttt actgctgctg
     2821 catcaattgc agaagaaagc cgtgagaaaa tctcatcaat gcttccaacg ccgtgaatct
     2881 cactgcattt gttttgttgc tgataataag cctttaccgg agtagtttcc gcattgtaca
     2941 catcaatccg tttctgaatc acattcggat cagcatcatc tgctcgtccc gatgtttttc
     3001 ctctttcaag caaacggcgt ttcaattctt cttcgggaac ttcaagcgaa agcataacgg
     3061 tgatgctctg accttttgaa tccagaaatg aatccagcgc ttctgcctgt gcggaggtgc
     3121 gtggaaaacc atcaaaaata aatccgcccg cagatggatt cttgtttact tccgactcca
     3181 gtaaacgtat cgttacttca tccggcacga gctgaccttt atcgatataa ctttttgcaa
     3241 gattaccgag ttctgttcca cccttaatgt tcgcacggaa aatatcaccg gttgagaggt
     3301 gaacaagttt gtactttgca atcagatttt ccgactgtgt acctttccct gcaccgggag
     3361 gaccaaataa cacaatgttc agcataggca tgattttaat tatgaaacga tctggtaaat
     3421 atctctgagg ttacgtccga gtccatcgta atcgagtccg tagcccacaa taaaagcatt
     3481 ggggatttcc atggcgacgt aatctatttt gtgttttccc ttgtaggctt cgggcttgta
     3541 aagcagtgtg gccactttaa ccgaagcagg agaatgtttc tgcaaggtat ccatcacttt
     3601 ttccagtgta aggccggtat ccacaatatc ctccacaaca ataatatcgc gaccacgaat
     3661 atcttctgcc aatccaacca attcccgcac ctcgccgctt gagcctgtgc cgatgtagga
     3721 agcaagtttt acaaaagtga tctgacaatc gcatttcacc tcgcgaataa ggtcagcagc
     3781 aaaaaggaaa cttccattga gtacaccaag caatagcgga gtacgaccgg cataatccgc
     3841 actgatgcgc ttgccaatct cagcaatgtt cccaagtatc tcctcctcac gcaggtaaag
     3901 ggcgaacgtc ttgtccttga gtgtcacctt catccgacaa aggtaacatt tttgaaattt
     3961 tcggccaact aactgataat ctgaagtcta acccgaaatt acgacttcag caacagctat
     4021 tcctcaggca gaagactgat catgcgctga atgaaaacgg cattaggaat agttaccgtt
     4081 gtgccttcaa tggttttgat ggtgataaag aacactccga tctcagagat agttccggta
     4141 acaggatagt ctttatccat gatctgaatt ttgcttccca cttttgcagg atgattgatg
     4201 aaaagcaaaa cagcggaggt gatatttgac aacaaagacc attgcgcaaa aaatgcaatt
     4261 ccaaggagag tgagcatgga agaaacgaag agaaacaatt cccgctgatc aacaccccag
     4321 atcatcaata atatgatcac tcccaaaaca ttgataaaaa cgcgaacgat acgtccaagc
     4381 atttttgctc tacggtgttc aatttcaatg gcagtagtaa cgcggcgaaa aacacgggta
     4441 acaattgcac gcaacacaat aaccactaat actactacca gtgtttccag catctggtat
     4501 tcgtaaggat gtaaaaattc gatcgttttc attggcgatt agcaaactat gattgggttt
     4561 gaaggaacat ccgcatctgc acaatgatca tcatgcaggc cagtaacaaa tgcaaaggtt
     4621 gaattagtgc aggcatattg aaataggcca gtgttgcacc gcttaccact tcaattgcaa
     4681 ttacagaaaa caagaatttt attccgggta attccaccga tgattcgcgc agcttgtacc
     4741 aaatgaaaat ggtcaacaat acaatcatcc acgaaaaaga acggtgaatg taaaatatgg
     4801 ttgtgagttg atcgatccac aaatgacgat cctctccacc gaattgcgcg tacaaagcat
     4861 cgatttgctg acgaatctgt gtccccatca atatctggag gagtccgatc acaattacaa
     4921 gtcccaataa aacacgaatc gttttgggta cctgaatttt ttctttctgc attgatgcac
     4981 gctctatgat gacaacctgc aaaacaatga tcagcaatgc aagaaccata tgtaccgtaa
     5041 gaacccaggg taagagattg gtagcaacca caatggcacc aaaccatgcg gttactgcaa
     5101 gcagaatcaa attgagcaaa cagaaaaaga tcaggcgctt atctgttttc caccaccaga
     5161 gcgaaagaat aaattgcacc aacatcaaat tacccgaaac aaatcctatt aagcgattga
     5221 tgtattctgt ccatgttttt gttgcgttaa aatcctgttc ctccaataaa gaagaatcgc
     5281 tacgcattgc agtggcttcc tgcgaaaaac cggtccggtc gagcagtcgg caaaaatcct
     5341 caagtttttt tgcgcgtttg gctgcgtaca cttctttgta gtcggcaggt aattgtgaaa
     5401 tatccgttgg aggaatccac tgcccgaaac aacgcggcca gtcgggacaa cccatgcccg
     5461 aaccactcat gcggacaaca ctacccgcca gaacaaccag cagaacagct atcagggtga
     5521 gcttcgtcag aattgagaat cgcttgatca
//
LOCUS       DAOMYG010000194         5543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5020_length_5543_cov_9.482690, whole genome shotgun sequence.
ACCESSION   DAOMYG010000194 DAOMYG010000000
VERSION     DAOMYG010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5020_length_5543_cov_9.482690"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PK610_07435"
     CDS             complement(<1..524)
                     /locus_tag="PK610_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35804.1"
                     /translation="MLEKFKSAYRFLSSKYQSIHLDYRVNPKPRYGHGNPPHKALYDS
                     INGNRKAYEELLRSFLKYDAQMHTIQDASLEKDQNKPAWNNGFLPGLDIVGIYGMIAE
                     YKPAKYIEIGSGNSTKVARKSITENHLKTEIISIDPFPRAAIDHLADRVIREPFENLS
                     DSRFIVETLNENDI"
     gene            complement(641..1168)
                     /locus_tag="PK610_07440"
     CDS             complement(641..1168)
                     /locus_tag="PK610_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35805.1"
                     /translation="MSRSLLVFFLFSLFSCMTTPQGDKRSAVDCVEILFFGKNTGDIA
                     GISIGDPSSAVMDARVGTPLYQDDLETIDSVSLCNEKNGHAELACRFVSGRLAEMELT
                     LFLKQETDLDPVFNLIKTTMDQRHDGGMQQQGVWFWQRNRERFLDQIYLLNASDRYKR
                     PVVQMLLSDGEGTPA"
     gene            complement(1187..1882)
                     /locus_tag="PK610_07445"
     CDS             complement(1187..1882)
                     /locus_tag="PK610_07445"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRJ35806.1"
                     /translation="MMQRAENILFLDIETVPLVYEFDRLDSGTQSLFDAKTRFGSREF
                     PDVNERYKKAGIYAEFGKIVCVSCGYYLFNRGKTELRIRSFAGDNEIDLLRQFCDFVT
                     KNFDEDFHRLCAHNGKEFDFPYLCRRMIVLGIPIPRILHLQERKPWEVPHIDTMELWK
                     FGDHKHYTSLDLLAHVLNVPSSKTDMNGSDVARVYWEEKDLQRIIEYCEQDVKTLALV
                     WERLCGVHAGIFV"
     gene            1998..2636
                     /locus_tag="PK610_07450"
     CDS             1998..2636
                     /locus_tag="PK610_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRJ35807.1"
                     /translation="MSDILEQIIRTRFKQFNEDALITEILEHGALHTCTTEEQIINPG
                     AYVKMVPLLVYGSIRISRQDEEGREIYLYHLYPGQTCAISLNCCMSMKPSEVLAIAED
                     ETEFISIPVQLLDEWTIKYKSWRNFMFETYRERFHELIQTIDNIAFHKLDERLVIFLN
                     EQIKAGKSKKLHLTHQQIAQSLGSSREVISRLLKQLERDGKVKLGRNEITMA"
     gene            2710..2991
                     /locus_tag="PK610_07455"
     CDS             2710..2991
                     /locus_tag="PK610_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRJ35808.1"
                     /translation="MNLQELVKDPKTVLIDVRTPMEYADQHIEGSLNIPLDTVPEEID
                     RIRNLNATVVLFCRSGARSGQALMYLQQAGIKNSYNAGGISNVMVLKMN"
     gene            2993..3301
                     /locus_tag="PK610_07460"
     CDS             2993..3301
                     /locus_tag="PK610_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRJ35809.1"
                     /translation="MFGLIKNLFGEKTDFRALMKEGAIIIDVRTPQEYKGGHIKGSQN
                     IPLYKIDAQVESIRKKGKPVITVCRSGGRSGMAADILRKKGIKVANGGPWNSLQRSIQ
                     "
     gene            3316..3516
                     /locus_tag="PK610_07465"
     CDS             3316..3516
                     /locus_tag="PK610_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35810.1"
                     /translation="MRIFRLALGAMIVGEGLHSGDSLLIVLGGVLIVQGILNMGCGFG
                     NTFTVHSDTKVEQEISYEKIDP"
     gene            3497..3709
                     /locus_tag="PK610_07470"
     CDS             3497..3709
                     /locus_tag="PK610_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HRJ35811.1"
                     /translation="MKKLILDRKKTILGIAAGAIAGWMYWYFIGCSNGSCGISSTWYM
                     STLYFSIGGGLLAESFSPAKKAEPKN"
     gene            complement(3714..>5543)
                     /locus_tag="PK610_07475"
     CDS             complement(3714..>5543)
                     /locus_tag="PK610_07475"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRJ35812.1"
                     /translation="VECYMDLAYYYFYLEKFQDSYHYCELARPHAERSGNDFRKLDVY
                     NTGGSALKALKRQKDALVLLHKAGDLLETTTSPIKRHLGSIYTNLGITYYELGEPAKG
                     FEYKVKALEFFRKTDNESGVFNVNIAIGIDLIEIEKKPELAVKYLHEAELKFKEHGSL
                     YTGQHLYRSLHQSYATMGNYEKAYQYFEKFLTIRDSSLNSEKQKQLGEMEAKYENSKK
                     EQQIEMLSRDQKIGQLMLLRNKEALEKEKAENQIKENSILLLEQESSLKEEQLAREKA
                     QTEAERNKAMLAQEQKKLAEKETQDQRRLNMMFGGASLLLIIFLISLYRGYRQKKKAN
                     EEIMQQKSIIELQKAIVEEKNKEVLDSINYAKRLQDAILPPIAYLTSLLRDSFILYKP
                     KDIVAGDFYWLEEVNNTRNEKLIYFAVADCTGHGVPGAMVSVVCSNALHRTVKEFGMT
                     DPGEILDKVTELVLETFSRSESDVKDGMDISLAVFNTNTKELFWSGANNPLWILREGN
                     ITEIKPDKQPIGSFDGRKPFTTHTIRPLAGDVFYLFSDGYCDQFGGEKGKKLKEANFR
                     KMLLAMNGASMQRQQQQLLTAFENWRGDLEQVDDVCVLGVRLF"
BASE COUNT         1611 a         1219 c         1115 g         1598 t
ORIGIN      
        1 aaaatatcgt tttcattgag tgtttccaca ataaaccttg aatcggataa attttcaaag
       61 ggctcacgaa taacacggtc tgccaggtga tctatggcgg ccctgggaaa gggatcgatg
      121 gaaatgattt cggtttttaa atgattttct gttatcgatt ttcgcgcaac ttttgtggaa
      181 tttcccgatc caatctcaat atactttgca ggtttatatt cagctatcat accataaata
      241 cctacaatat caagtccggg taaaaacccg ttgttccagg caggcttatt ttgatccttc
      301 tccaacgatg catcctgaat tgtgtgcatt tgcgcatcgt atttcaaaaa tgaccgcaac
      361 agttcctcat aggctttcct gtttccattt atgctatcat acaaagcttt gtggggtgga
      421 ttgccatgac cgtatcgcgg cttcggatta acacggtaat ccagatgaat gctctggtat
      481 ttggaagaaa gaaaacggta cgcagatttg aatttttcca gcattgcaac agtagagtta
      541 gttcatcatc agcattctgc cgatcacatc aattatgact ctaaaattag cacaaaaaac
      601 tgaggggaac atctttcaaa cccatctatt gagccgatct tcaggcagga gtgccttctc
      661 cgtcggacaa gagcatttga acaacgggtc ttttatagcg atcactggca ttcagcaaat
      721 aaatctgatc aagaaaacgc tcacgattgc gttgccaaaa ccaaacaccc tgttgttgca
      781 ttcccccatc atggcgctga tccattgttg ttttaatgag attgaaaacg ggatcgagat
      841 ccgtctcctg ttttaaaaac aatgtcagct ccatctccgc gagtcgaccg gaaacaaagc
      901 gacaggccaa ttcagcatga ccattttttt cattgcacag cgacaccgaa tcgatagttt
      961 ccagatcatc ctgataaagc ggcgttccta cccgtgcatc cattactgcc gaggaaggat
     1021 ctccaatcga aattccggca atatctcctg tgttttttcc aaaaaaaaga atctccacac
     1081 aatcaactgc agagcgtttg tcgccctgag gcgttgtcat gcatgaaaac aaggagaaaa
     1141 ggaagaacac caacaaagac cgggacataa actacttttt gcaaaactat acaaaaatcc
     1201 cggcatgcac accgcaaagc cgctcccaaa cgagtgccag ggtcttcaca tcctgttcgc
     1261 aatattcaat gatgcgttgt aagtcctttt cctcccagta aacacgtgcg acatcgcttc
     1321 cgttcatgtc tgttttcgaa ctcggcacat taagtacgtg ggcaagaaga tcgagagagg
     1381 tgtagtgctt atgatcgcca aatttccaaa gctccatggt gtcaatatgc ggaacttccc
     1441 atggttttct ttcctgcaaa tgaaggatgc gcggaatggg tataccaagt acaatcattc
     1501 gtctgcacag ataggggaaa tcaaattcct ttccgttatg cgcacacaag cgatgaaaat
     1561 cttcgtcaaa attcttcgta acaaaatcgc aaaattgcct gagaagatct atttcattgt
     1621 cacccgcaaa agaacgaata cgcaattcgg tttttccgcg gttaaagagg tagtatccgc
     1681 acgatacaca gacaattttc ccaaattccg cataaatccc tgctttctta taccgttcgt
     1741 tcacatcagg aaattcacgc gaaccaaaac gggtttttgc atcgaataag gactgcgttc
     1801 cggaatccag gcgatcaaac tcatagacaa gtggcacagt ttcaatatca agaaacaata
     1861 tgttttcagc tctttgcatc atgaaacaaa catacaagga tcaggaacaa gttgcagacc
     1921 aaagtgagtg cgccgattaa gattcggtcg gaaggaatag ggtaaatttt cttactttta
     1981 gagcataaat aagaaccatg tctgatattc tcgaacagat catacgtacc cgttttaaac
     2041 agttcaacga agacgcgcta attaccgaaa ttcttgaaca tggtgcattg cacacatgca
     2101 caaccgaaga gcaaatcata aatcccggag catacgttaa aatggttccg ctacttgtgt
     2161 atggttccat acgcatcagc agacaagatg aagaggggcg ggaaatttat ctgtaccatc
     2221 tctatcccgg tcaaacctgt gctatctcgc tcaattgttg tatgagcatg aaaccaagcg
     2281 aggttttggc aatagcagaa gatgaaaccg aattcatttc cattccggta caattactcg
     2341 atgaatggac catcaaatat aaaagctgga gaaattttat gtttgagacc tacagagagc
     2401 ggtttcacga attgattcag accatagata atattgcttt tcataaactg gatgaacgcc
     2461 tggtgatttt tttgaatgag caaatcaaag cgggaaaatc aaaaaaactt catctcaccc
     2521 atcagcagat tgcacaatcg ctggggtcat cacgtgaagt aatttcacga ttactgaaac
     2581 agctggaacg tgacggcaaa gtaaaactgg gaagaaacga gattacaatg gcctagtacc
     2641 tgctttgtga gatttgtcac tgccggattt tgcagggcat cctacctttg cactataaaa
     2701 tttagacgta tgaatctcca ggaattggta aaagacccaa aaacggtttt aattgatgta
     2761 agaaccccaa tggaatatgc agaccagcat attgaaggtt cattgaatat tcctctggat
     2821 actgttccgg aagaaattga ccgcatccgc aacctgaatg caacagttgt attgttttgt
     2881 cgctccggcg cacgcagtgg tcaggccttg atgtatctgc agcaggcggg tattaaaaac
     2941 agctataatg caggcggaat cagtaatgtg atggtcttaa aaatgaatta atatgttcgg
     3001 actgatcaaa aatttatttg gcgagaaaac ggattttcgt gccctcatga aagaaggcgc
     3061 aatcattatc gatgtgcgaa cgccacagga atacaaaggc ggtcacatta aaggatcgca
     3121 aaatatccct ctttataaaa ttgatgcaca ggttgagagc atccgaaaaa aaggaaagcc
     3181 ggtaatcact gtttgccgct ccggcggaag aagcggaatg gctgctgata ttcttcgcaa
     3241 aaaaggcatt aaagtggcca atggtggtcc ctggaacagt ttgcagcgaa gcatacagtg
     3301 aagcaactct cgtttatgcg tatttttcgc ttagcacttg gagcgatgat cgttggtgaa
     3361 ggacttcact cgggcgattc ccttcttatt gtgcttggag gagtgctaat cgtgcagggt
     3421 atattaaata tgggttgcgg gtttggaaat acctttactg tgcattcaga tacaaaagtg
     3481 gaacaagaaa tcagctatga aaaaattgat ccttgatcgg aaaaaaacaa ttttaggaat
     3541 tgcagcagga gcaattgcgg gttggatgta ttggtatttt attggttgca gtaatggatc
     3601 atgtggtatt tcatcaacat ggtacatgag cacactctat ttttcgattg ggggcggatt
     3661 gttagccgaa tctttttcgc cggcaaaaaa agctgaaccg aaaaattaaa ttttcagaac
     3721 aagcgaaccc ccaatacaca aacatcatcc acctgctcca aatcgcctct ccagttttca
     3781 aacgcagtta gtaactgttg ttgctgcctc tgcatggaag ctccattcat ggcaagcagc
     3841 atttttctga agttcgcttc tttcagtttt tttccttttt ctcctccaaa ctgatcgcag
     3901 taaccatctg aaaaaagata aaacacatca cctgcaagtg gccggatagt atgcgtcgta
     3961 aatggtttcc tgccgtcgaa actaccgatg ggttgcttgt ctggttttat ttctgtgata
     4021 ttaccctcac gcaaaatcca taaaggattg ttcgctcctg accaaaataa ttccttcgta
     4081 tttgtattaa atacagcaag agaaatatcc attccatcct tcacgtcact ttcagatcgg
     4141 gaaaatgttt ccagaaccaa ttccgtcacc ttatccaata tttctccggg atctgtcatt
     4201 ccgaattctt taactgtacg atgtaaggca ttggaacaaa caacactcac cattgcaccc
     4261 ggtactccat gtccggtgca atctgcaact gcaaaataga tcagtttctc attgcgggta
     4321 ttgttgactt cttcgagcca gtaaaagtcg cctgcaacaa tatcttttgg tttatagaga
     4381 atgaaactat cccgcaacaa agaagtgaga taggcaatag gcggtaaaat ggcatcctgc
     4441 agtcgttttg cgtaattgat ggaatcaagc acttctttgt tcttttcttc cacaatcgct
     4501 ttttgcaatt cgatgattga tttctgttgc atgatctctt cattcgcctt tttcttctga
     4561 cgatatcccc ggtagaggga aatcaggaat attatgagca ataatgatgc tccgccaaac
     4621 atcatattta atctgcgctg atcctgtgtt tctttttcag caagtttttt ctgctcttgg
     4681 gcaagcatcg ctttgttacg ttctgcttcc gtttgcgcct tttcacgtgc gagttgttct
     4741 tcttttaaac tggactcctg ctccagtaat aaaattgaat tttccttgat ctgattctcg
     4801 gctttttctt tctctaaggc ctctttgtta cgtaacagca tcaactgacc aattttctgg
     4861 tccctgctga gcatttcaat ttgctgttcc ttttttgagt tttcatactt cgcttccatc
     4921 tcccccaatt gcttttgctt ctcggaattc agggagctat cacgaatcgt tagaaatttt
     4981 tcaaaatact gataggcctt ttcataatta cccatagtcg catatgactg atgcaatgat
     5041 cggtaaaggt gctgaccggt ataaagcgat ccgtgttcct tgaattttaa ttccgcttca
     5101 tgcaggtatt tcactgccaa ttccggcttt ttttcgatct caatcaaatc aattccaatg
     5161 gcaatattca cattaaacac cccggattca ttgtctgttt tacggaagaa ttcaagcgcc
     5221 tttaccttat attcaaatcc ttttgcaggt tcaccaagtt cgtaatacgt aattccaaga
     5281 ttggtataaa tagaccccag atgccgcttg attggagatg ttgtggtctc gagcaaatcg
     5341 cctgccttat gcaataacac aagtgcatcc ttctgtcgtt taagcgcctt aagggcagag
     5401 ccacccgtat tatacacatc cagcttacga aaatcattac cggaacgttc tgcatgcgga
     5461 cgagccaatt cacaatagtg gtacgaatcc tgaaattttt ccagataaaa ataatagtag
     5521 gcgagatcca tgtaacattc cac
//
LOCUS       DAOMYG010000195         5537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5028_length_5537_cov_8.295695, whole genome shotgun sequence.
ACCESSION   DAOMYG010000195 DAOMYG010000000
VERSION     DAOMYG010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5028_length_5537_cov_8.295695"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PK610_07480"
     CDS             complement(<1..385)
                     /locus_tag="PK610_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35813.1"
                     /translation="MQTTVMKNVLLVFGLIFSLQASAQHPLIDSVLVRVRIDSLMHYA
                     EQISGEKGVVINGVTDTIKTRHKLKAGNELAYRFLIEKMGQYNVQIDSMVFSSTGKNI
                     LAIQPGAVYPNIYVILCAHYDSMPNS"
     gene            complement(405..>1309)
                     /locus_tag="PK610_07485"
     CDS             complement(405..>1309)
                     /locus_tag="PK610_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HRJ35814.1"
                     /translation="SPFTVHRSPFTVHRSPKMKHALVIQGGGFRTAFSSGVLDAFLEE
                     EYFPFDIIAAVSGGAIAASYFIAQQKEHCFRSICFLSEKGRFVNINRALRAGPLMDID
                     IFHAISNKHFPFDKTLATERLIGKKFAIVMTNRANGQPHYCDPTTTNWEEAVIASCSL
                     PFITKGKQHLNGNDYIDGAWGDPLPVEWAAEQGATTITVVRTSPPFERQNKSWFDRIG
                     ELYYLKNPSLRNAFSENHLRFNRSIDFIENPPDGIAIHQIAPDFALKAGLYTQSKEHL
                     EDDYRYGFALGKQYCTMQQTVISE"
     assembly_gap    1310..1409
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1596..1820
                     /locus_tag="PK610_07490"
     CDS             1596..1820
                     /locus_tag="PK610_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HRJ35815.1"
                     /translation="MAKTKITINNNGSLRVEGEFEIVDKNGNAYDLAGREVIGLCRCG
                     HSKNKPFCDGSHNGNFSHEAVAFALPPKKV"
     gene            1904..2362
                     /locus_tag="PK610_07495"
     CDS             1904..2362
                     /locus_tag="PK610_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35816.1"
                     /translation="MFDEDFNDDYFSGYEAKNLEESIRKHPLYLQAKNLHDVCGSITE
                     LLNGAEAEFSKRELQESGLVILSKTYAMLTIDSKLLRLVEASVLRMYAERIRISSLEL
                     KMFNRVDLQYSTLLRTELELYRAHFLDLMKETHDMSCEEDFEDDWGVIKC"
     gene            complement(2395..3345)
                     /locus_tag="PK610_07500"
     CDS             complement(2395..3345)
                     /locus_tag="PK610_07500"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRJ35817.1"
                     /translation="MSEYLHDHKNFPDLILILAEEKGIDPGLVEKDYWIMHVLYGLSK
                     NGFQFELKGGTSLSKGYKLIHRFSEDIDIHICPPASFKINENPKNSNKNNAEKRKNFF
                     DWLAQEISIPGIINSKRDPVFDDLELYRSGGIRLQYNTTFGQIEGIKEGILLEVGFDT
                     VAPNERLTISSWAIEKALENKVEIIDNRATGIPCYDPRYTFVEKLQTIATKFRNEQDT
                     GKKGMNFMRQYYDVFCLLGEPMIIDFIGSNEYQNHKRIRFPDADYEIPIHKNEAFILS
                     SPLQKSDFEKRYSSTASLYYQNQPSLDEILERIALHAHRL"
     gene            complement(3338..3928)
                     /locus_tag="PK610_07505"
     CDS             complement(3338..3928)
                     /locus_tag="PK610_07505"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HRJ35818.1"
                     /translation="MSGTEKLKKHLKVGHVYRREDLCKWSTAIDRHLEELVENGQLEK
                     LARGLYYVPKTSVFGKVPPAENFMIEGFLKDDRFLLVSPNLYNTLGLGTTQLYNKKIV
                     YNHKRHGKFRLGNREFEFRRKYAFPSKLSPEFLLIDLANNLNELAEDKDELIKKIRSK
                     ASQMPPSTLRLMVKKYASMKTKKMLAPLLNSSDKDV"
     gene            complement(4199..5275)
                     /locus_tag="PK610_07510"
     CDS             complement(4199..5275)
                     /locus_tag="PK610_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35819.1"
                     /translation="MRILVFLLFCSSFLRGNCTGENEAYYFNIGGGFAPLLLADLDSV
                     TRIKKTWQFLDTLKVLSPKGEAEHGFFIPVPMTFNATQPAETIEPIGFQRFRMGPINS
                     FRRDSIVFNGKTYRIYLHHTDDCFVGGRINHVTNTMWVETLGMVHSYTNFSGDHNMIL
                     FRCRDSVKQELLTAVLERMEKDYLVHFGYSYKKLLDRGMSAPFDDLPQAWYTEWKSAQ
                     SHLRLQSAEYTDNITHVTMKARIKNVSDLNYFINSECYIGPALAMVHYGEKAHPWNLL
                     ETHYGTHFTRALKDIILKPGEEHEFSFTFRKHNGCGGCTNVVYSGFQLYPHESRLAHW
                     LLGEPFEKNGVMVYMFPHREVKGE"
BASE COUNT         1491 a         1108 c         1227 g         1611 t
ORIGIN      
        1 tgctgttcgg catactgtcg taatgggcgc agagaatcac ataaatgttc ggatatacag
       61 caccgggttg aatggcaagt atgttttttc ctgtggatga aaacaccatc gagtcgatct
      121 gcacgttata ctgacccatc ttctcaatca gaaagcgata ggcaagctca tttcccgctt
      181 tcagtttgtg tcttgttttg atagtatcgg ttacgccgtt aatcaccacg cctttttcac
      241 cggagatttg ttcggcataa tgcatcagac tgtcgatacg tacacgcaca agcacagaat
      301 cgataagcgg atgttgtgcc gatgcctgca acgaaaagat caaaccaaaa acaagcaaca
      361 catttttcat aaccgtcgtt tgcataaatc taggcttttt tttgctattc cgaaattaca
      421 gtctgctgca ttgtgcaata ctgctttcct aaggcaaatc catagcgata atcatcctcc
      481 aggtgttctt tggattgggt atataaaccc gcttttaatg caaaatccgg cgcgatctga
      541 tgaatcgcaa ttccatccgg aggattttct ataaaatcga tggaacgatt aaaacgcaaa
      601 tgattttcgg aaaatgcatt tcgcaacgat ggatttttca gataatacaa ttcgcctatt
      661 cgatcgaacc aggatttgtt ttgtcgctcg aagggtggag atgtacgcac tacagtaatg
      721 gttgttgccc cttgctctgc tgcccactca accggcaaag gatctcccca tgcgccatcg
      781 atgtaatcgt ttccgttgag gtgttgtttg cctttggtga tgaacggaag tgaacaggaa
      841 gcaatgaccg cttcttccca attggtagtt gtgggatcgc agtaatgggg ttgaccgttg
      901 gcgcgattgg tcattacaat ggcgaatttt ttcccgataa gacgctcagt ggccaatgtt
      961 ttatcaaaag gaaaatgctt attggagatc gcatgaaaaa tatcaatgtc catgagcgga
     1021 ccggcacgca aggcgcggtt gatgtttacg aagcgaccct tttcagagag aaaacaaatg
     1081 gaccggaagc aatgttcttt ttgttgcgca ataaaatacg acgcagcaat tgcaccacct
     1141 gaaacagcag cgataatatc gaaaggaaaa tattcttctt ccagaaatgc atcaagcact
     1201 ccagaactga aagctgtccg gaatccacca ccttgtatga cgagtgcatg tttcattttt
     1261 ggtgaacggt gaacggtgaa cggtgaacgg tgaacggtga acggtgaaan nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gtgaaaagtg aaaagtgaaa agtgaaaagt
     1441 gaaaagtgaa aagtgaaaag tgaaaagtga aggagattgt ttgaatttac taaggtaata
     1501 aattttcaaa cggtattttt tggtggagga ggttgaattc attttatggg gattgacggg
     1561 aattgagtat tttggtgcaa cgaaaacaaa acatcatggc aaagacaaag attacgatca
     1621 acaacaacgg ttcactgcgt gtggaggggg agtttgaaat cgtggataaa aacggaaacg
     1681 catacgattt ggcaggacgc gaagtgatcg ggctttgtcg ctgcggacat tccaaaaaca
     1741 aacctttctg cgacggttcg cataacggga attttagtca tgaagcagtc gcttttgcgc
     1801 tgccgcctaa gaaggtgtaa gggttcaccg aaatgtaatt caggcgaaat attggttttt
     1861 ttgtcgggta aatttttctg tggtttgcag gaaaaaatac accatgtttg acgaagattt
     1921 taatgatgat tatttttcgg gctatgaggc taaaaacctg gaagaatcaa tccgtaaaca
     1981 tcccttgtac ctgcaggcaa aaaaccttca tgatgtctgt ggctcaatta ctgaactctt
     2041 aaatggcgcg gaagccgagt tttccaaaag agagctgcag gagtcgggtc tggtgattct
     2101 ctctaagact tacgcaatgt tgaccattga ttcgaaacta ttgcgtctcg ttgaagcttc
     2161 cgttttgcga atgtatgcag aacgcattcg gatttcttcc ttggaattga agatgtttaa
     2221 ccgtgtcgac cttcaataca gtaccttgct ccgaacggaa cttgagcttt acagagctca
     2281 tttcctcgac ttgatgaagg aaacacacga tatgtcatgc gaagaggact ttgaagacga
     2341 ttggggtgtt attaaatgtt gatgccaaaa ttggatcgta gtaatgaagc agcatcagag
     2401 acggtgagcg tgcaatgcaa ttctttccag gatttcatcg agacttggct gattctgata
     2461 atatagactg gcagttgatg aatatctttt ttcaaagtcc gatttttgta aaggagaaga
     2521 aaggataaaa gcctcgtttt tgtgaatcgg aatttcgtaa tcagcatccg gaaaccgaat
     2581 acgtttatga ttttggtatt cgttgcttcc aatgaaatcg attatcattg gttcgccgag
     2641 caggcagaaa acatcataat attgtcgcat gaagttcatg ccttttttac ctgtgtcttg
     2701 ttcgtttctg aacttggttg caatggtttg tagcttttca acgaaggtgt atcgcggatc
     2761 ataacatggg attccggtgg ctctattgtc aatgatttcc actttgtttt ccagtgcttt
     2821 ttcaatagcc caggacgaaa tcgtcaatcg ttcatttgga gctacagtat caaagccaac
     2881 ttcaaggaga attccttcct taatcccctc tatctgaccg aacgtagtgt tgtattggag
     2941 gcgtattcct ccgctcctgt acaattccaa atcatcaaat accgggtctc tttttgaatt
     3001 gataataccg ggaatgctaa tttcctgggc taaccaatca aagaagtttt tcctcttttc
     3061 tgcgttgttt ttatttgaat ttttaggatt ctcatttatt ttaaaagatg caggaggaca
     3121 aatatgtata tcaatgtcct cagaaaacct atggatgagt ttatagcctt tcgatagcga
     3181 tgttccgcct tttagttcga attgaaatcc atttttgctc aacccgtaca agacatgcat
     3241 gatccagtaa tctttttcaa ctaagcccgg atcaattcct ttttcttctg caagaatcag
     3301 aatgaggtcc gggaaatttt tatggtcgtg caggtattca gacatcctta tccgatgaat
     3361 tcaaaagtgg agcaagcatt ttttttgttt tcatgctggc atacttcttc accattaacc
     3421 gcaaagtgct tggcggcatt tgtgatgctt tggatcgtat tttttttatc aattcatcct
     3481 tatcctcggc tagctcattc aggttattgg ccagatcgat caaaagaaac tccggactaa
     3541 gtttagacgg gaatgcatac ttgcgtctga attcaaattc acggtttcct aatctgaatt
     3601 tgccgtgtct tttatggttg tacactattt ttttattgta cagttgcgta gtgcccaggc
     3661 caagtgtatt ataaagatta ggggacacca aaagaaaccg atcatccttg agaaaccctt
     3721 cgatcatgaa gttttctgca ggaggcactt ttccaaagac agaagtttta ggcacataat
     3781 acaaacccct tgcaagtttt tccagttgcc cgttttcgac caactcctca agatgcctgt
     3841 cgatggcggt tgaccacttg cataggtctt cccttcgata cacatgtccg acttttaaat
     3901 gctttttaag cttttctgtt ccgctcatat cctgtttatg gttggatgaa agtaaaaatt
     3961 tacttgctta tacgcaagta gttttagtaa agtttcatgc atttgaatcc aataatgtat
     4021 taaaatacaa tagtttagat tgttttaaat ggcatagatt tttttgtctc tgtcgttttc
     4081 aaagacagaa gataacgtag cgtatcgata tccctgtaag taggtcaatc cgatccggtt
     4141 gccgtggaaa ttacaactta aaaccggtca accttattca ggcattgcaa cgagatttct
     4201 actcaccctt cacctcccta tgcggaaaca tatacaccat cactccgttt ttctcgaagg
     4261 gttcgccgag cagccagtgg gctaaacgcg attcgtgggg gtagagttga aatccactgt
     4321 acaccacatt ggtgcagcca ccacagccgt tgtgttttct gaaggtgaat gaaaattcat
     4381 gctcttcacc gggtttcaga atgatgtctt ttagcgccct ggtgaaatgt gttccatagt
     4441 gcgtttccag caaattccag gggtgcgctt tttcgccgta atgaaccatc gctaaagcag
     4501 gaccgatata gcactcggaa ttgataaaat aattgagatc cgaaacgttt ttgatgcggg
     4561 ctttcatggt aacatgcgtg atgttgtcgg tgtattctgc cgattgcagc cgcagatgtg
     4621 attgagccga tttccattcg gtgtaccagg cttgtggtag atcatcaaac ggagcggaca
     4681 tccctctatc cagtagtttt ttatacgagt agccgaaatg taccaggtaa tctttttcca
     4741 tgcgttcaag caccgcagtg agaagttcct gcttaacgct gtccctgcaa cggaacaaaa
     4801 tcatgttgtg atcaccggaa aaattggtat agctgtgcac catcccgagc gtttctaccc
     4861 acatggtatt ggttacatga ttgattcgtc cgcccacaaa gcaatcgtcg gtatggtgca
     4921 aatagatgcg gtaggttttg ccattaaaaa cgatgctgtc ccgacgaaat gaattgatgg
     4981 gccccattcg gaagcgctga aaaccgatgg gttctatggt ttctgcaggt tgagttgcat
     5041 taaacgtcat cggaacggga ataaagaagc catgttcggc ttctcccttc ggggataaca
     5101 ccttaagggt atccaaaaat tgccaggttt tcttaatacg ggtaacgctg tccagatccg
     5161 ccagcaacag aggggcaaac ccgccgccga tattgaagta atacgcttcg ttttcgccgg
     5221 tgcaattgcc gcgcaaaaac gatgaacaga acagaagaaa tacaagtatc cgcattgttg
     5281 ttgttttgtg atgcggtaca ctcgggggga ttcccggttc aaatcatcag acttgcaaaa
     5341 gctgtcggtg acgttatttt gatggggttg atttatgcaa gtctccacag tgaagtgaaa
     5401 ggccattgcg aaataaaaaa ggtccgaaga acactcttca ggcctttcta cttaactgta
     5461 cccggggtcg gactcaccac cagtacttcg actgttggtc aagccctcct gaatgacttc
     5521 agtcgggcag gcttgtc
//
LOCUS       DAOMYG010000196         5531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5035_length_5531_cov_9.337107, whole genome shotgun sequence.
ACCESSION   DAOMYG010000196 DAOMYG010000000
VERSION     DAOMYG010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5035_length_5531_cov_9.337107"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PK610_07515"
     CDS             complement(<1..752)
                     /locus_tag="PK610_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35820.1"
                     /translation="MKKHLLLLLVSLLFGGIHAIGQTYTMGTAPAGNNATINTCSGTF
                     FDSGGSGGAYGNSQNFVVTICPSTPGQMIQLNITSFNTEANFDYLTLFSGTGTGGPIL
                     VGPVSGTAMPNPSTVVSQAANGCVTIRFTSDGSVTAAGFSIGISCVTGPTCTDGIQNG
                     QEAGVDCGGCSTCPPCPGSGITNATVTAASNIINLPCGGGNVNLSAVGNSTTVQLGSN
                     FNSGTPGPGWSVSPAGQFNNPCGNGPAGTGAH"
     gene            complement(772..1212)
                     /locus_tag="PK610_07520"
     CDS             complement(772..1212)
                     /locus_tag="PK610_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35821.1"
                     /translation="MRNFLLLLFVFVAFSVNAQDNKKADWTFLKAEKGIKVYYQYVDC
                     DFNIAPDAQYVLLKFENTLGMPVYVEYEQLLWYNGHCGLCTSDPNGESKKQIKVAANG
                     TTEGKCDETGDSSLKIFVKFNAFQTPDLLTKFEINNIIVTPISE"
     gene            complement(1439..3148)
                     /locus_tag="PK610_07525"
     CDS             complement(1439..3148)
                     /locus_tag="PK610_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35822.1"
                     /translation="MFQVLFSDCDTVQDVQRKLFRFFVLVFFFALVFLLSGGYVKAQS
                     VVVSQYFNAANEGDEWTELLVIDDNVDMRSWTIRDNGGSQGAWQTAITFRSIAFWQNL
                     RAGTVIILFHRTNNSGGSPNPVDINPSDGFLMLAVENSTYFSGGTFSSGSSMSIGGTG
                     DIVQLRNASSTHIHALSHRSTSGPDWTAITGPKLNHQNSASSGDAIFVCPGNSLSEYG
                     TDSPQDGTTWTTRNSTNLTFGLPNFCGGTSTPNSTFWRSLRGPQMTAQTVSASTSPTY
                     PTAALTFTWNSATDPVPGDNTTGYMVVRSSVGVFGTPVDGTTYTVGATIAGGGEVIAY
                     VDNTSSATHSYTDLSANDNGTYCYRVYAYRFGTDNINGNSFDVARGRAYNTTNFVDVV
                     CASNPLPVELLYFIGSVNNGKGNLNWSTATEHNNHFFIIDYSENGNDFSELVRVTGAG
                     NSSFPNIYSVIDDQLRNGQVFYRLFQVDFDGATRMEAQIVLDYFSEKIEVRFHKGLLT
                     ISNPNDEQLALEVFLMDGRKIVETLFRGDFSQSFETWSNTFMIYRLTSRTAVYSACIL
                     HLN"
     gene            complement(3160..>5531)
                     /locus_tag="PK610_07530"
     CDS             complement(3160..>5531)
                     /locus_tag="PK610_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ35823.1"
                     /translation="TLFFSWIIFFFGIVNAQVPTQCLEIESILVDACVPGSGCTSAAS
                     SNCSCEGKNEMVRFIVGPSPIPIGSLTVTWPNNSFLGIATPNATTAFLVATLQSTVQS
                     PCGRLMEPPGGIIPAGKRVLLITSTDMCTGANSFAGLQDTLYVIFQNPGNFQGHFANH
                     NNSGTATAAPTGASSLRTLSIGVSSPVCSDIVAYDRTLLVNQSGTYGGAAALNDGSRV
                     DFNFLGTPTYVNEGCTAPFVSTTLDISLTQINACFGDTLTINAIVDGFSQGIQWTGGN
                     GVFMNASSLSASYIVSASDAVNFYLYVLVSGTCSLPVKDSVLIHLTPLPQPEILVNGS
                     LSFCNGGAVNLSAGGVSSFSWSTGAVASSISVSVSGMVYLSSTNVCGTVFDSVEVEVF
                     SNPLVSFSLSPSICINDSPLSLIASPVGGVFSGTGVSGTMFDPSAAGSSTHFITYQFT
                     DGNGCSASSVQSITVIDVGAAQINDPGAICENAFVLNLTATPSGGVWSGSAITNPATG
                     LYDPALAASGSDTVIYIVSISGCSVSDTLIISLLEFPTVQITSSDTIILCSGQSATLQ
                     ATGTGVLTWSNGVTGNSISVSTTGNYSVSVSNACGTVTDGPVLVLVETVVASISPSSL
                     SGNVPLAGNFIATNSPGASFAWISNTGGSGSGAVYSETFSETGTFSVVLIATSPNGCI
                     AVDTVWVYAQELIEDSLYLFIPNVFSPNGDGQNDRFKILSYNYSVLEIEIFNRWGVSL
                     LFYDAKQTDWDGEFLESEAAEGVYYYIVKVVSVSGDTKVYSGTITLIRN"
BASE COUNT         1740 a         1218 c         1107 g         1466 t
ORIGIN      
        1 atgtgggcac cggtaccggc aggaccattt ccgcacggat tattaaattg acctgcaggt
       61 gaaaccgacc aaccgggacc aggagttccc gaattaaaat tacttcctaa ctgtaccgtt
      121 gttgaattac caacagctga aaggttcacg tttccaccac cacaaggaag gtttataata
      181 ttggatgcgg ctgtaacagt tgcattggtt attcctgaac cgggacatgg tggacaggta
      241 gaacatccac cgcagtctac accggcttcc tgaccatttt gaattccatc tgtacaggtt
      301 ggtccggtca cacaagaaat tccaatagaa aatccagcag cagttacaga accatctgaa
      361 gtaaatcgaa tcgtaacaca accattagct gcctgtgaaa caacagtact gggatttggc
      421 atggcggttc ctgaaacagg acctaccaat attggtcctc cggtacctgt acctgaaaac
      481 aaagtcaggt aatcgaaatt tgcttccgta ttaaaagaag taatatttaa ttgaatcatt
      541 tgtccggggg tacttggaca aatcgttaca acaaaattct ggctattacc ataggctccg
      601 cctgaaccac cagagtcaaa aaaggtacct gaacaggtat ttattgtggc gttatttcct
      661 gctggggctg tacccatggt gtaggtctgg ccaattgcat gaattccccc gaacaggaga
      721 gaaacaagca gtagaagtag gtgctttttc ataaggcgtt ctgttagggg attattcgct
      781 aattggtgta acaataatat tgttgatctc aaacttggtc aaaagatcag gtgtttgaaa
      841 tgcattgaac tttacaaaaa ttttgagtga ggaatcacct gtttcatcgc attttccctc
      901 agtggtgccg ttggctgcta cctttatctg ctttttcgat tcaccgttag gatcactggt
      961 acaaagtcca caatgaccgt tgtaccaaag cagctgttcg tattcaacgt aaacaggcat
     1021 acccaatgtg ttttcgaatt tcagcaatac atactgcgca tccggagcga tgttaaaatc
     1081 acaatctaca tattggtagt agactttgat gcccttttca gctttgagaa aagtccaatc
     1141 ggcttttttg ttatcctgag cattcacaga gaatgcaacg aaaacaaaca ggagtagtaa
     1201 aaaattgcgc atagtattgg ttttgatgaa agacggagaa tctattatag gttgcctgaa
     1261 aataagggta aaacaaatta attgtagtgc ttctacgatt ctcttatccg gcaggtttca
     1321 taggaggatt taaacctcac tgtttttcaa tcaattacaa gaggatcaaa ttccaacccg
     1381 ttattttgta accatttatg ggcaagttat ttttccgatt tgagggggta gcccctcatc
     1441 aattcaaatg tagtatgcag gcagaatata ccgctgtcct gctggtcaat cgataaatca
     1501 tgaacgtgtt gctccatgtt tcaaatgatt gagaaaaatc cccgcgaaat aaagtttcaa
     1561 ctatttttct gccgtccatt agaaatacct cgagggctaa ttgttcgtca tttggattgg
     1621 aaatagtcaa taacccttta tgaaatctga cttcaatttt ttcagagaaa taatccaaaa
     1681 ctatttgtgc ttccattcgt gtcgccccat cgaaatcaac ctgaaataaa cgatagaaca
     1741 cttgaccatt tctcaactgg tcatctatta cggaataaat gtttggaaaa gaagaattac
     1801 cggcacctgt aactctgact aattcagaaa aatcattgcc attttcggaa taatcaataa
     1861 tgaaaaaatg gttgttatgt tctgttgcag ttgaccagtt taaatttccc tttccgttat
     1921 taacactccc aatgaagtac aacaattcaa cgggcaatgg attacttgcg caaacaacat
     1981 caacaaaatt tgttgtattg tatgcacgcc ctcttgcaac atcaaaacta ttcccgttaa
     2041 tattatccgt accaaatcga taggcataaa cacgatagca ataggttcca ttgtcgtttg
     2101 cagataaatc ggtgtacgaa tgagtagctg atgaagtatt gtctacataa gcaatcactt
     2161 ctcctccacc ggcaattgtg gcgccaacag tatacgtagt tccatcaact ggtgttccga
     2221 atacaccaac actgctccga acaaccatgt agcccgttgt attatctcca ggaacaggat
     2281 cggttgcaga attccaggtg aaagtgagcg ctgctgtagg ataggttggt gaagttgatg
     2341 cggaaacagt ttgtgctgtc atttgcggtc cgcggaggga acgccagaaa gtagaattgg
     2401 gagttgaagt cccaccacaa aagttgggta gtccaaatgt cagattggta ctgttgcgag
     2461 tcgtccaggt tgtaccgtcc tgtggactat cagtaccata ttcactcaac gaattgcccg
     2521 gacaaacaaa aattgcgtca cctgaactag cactattctg atgatttaat ttcggtcctg
     2581 taattgctgt ccaatccggt cccgatgttg acctatgaga caaagcgtga atatgagtgg
     2641 aagaggcatt cctcaactga acaatgtcgc cagtccctcc tatcgacatt gatgacccgg
     2701 aactaaatgt tccaccagaa aaataagtgg aattctccac cgcaagcatc aaaaatccat
     2761 ccgatggatt aatatcgacc gggtttggcg atccacccga attatttgtt cgatgaaata
     2821 aaattataac tgttcctgca cgaagatttt gccaaaaggc tatgcttcgg aaggtgattg
     2881 cagtttgcca tgctccctga cttcccccat tatctctaat tgtccaactt ctcatatcaa
     2941 cattatcatc aatgaccaac aattcagtcc attcatcccc ttcatttgct gcattaaaat
     3001 attgcgacac caccacgctc tgcgccttta catacccacc actcagcaaa aaaaccaatg
     3061 cgaaaaaaaa gaccagcaca aaaaatcgaa atagtttgcg ctgcacatcc tgtacagtat
     3121 cacaatcgct aaaaagaact tgaaacattc gctccataat caatttcgaa ttaatgtaat
     3181 cgtgcctgaa tagacctttg tatcccctga aacagatact actttcacta tataataata
     3241 aaccccttcg gcagcttcac tctctaaaaa ttcaccgtcc cagtccgttt gtttggcatc
     3301 ataaaaaaga agtgatacac cccaccggtt gaaaatttca atctccaata cactgtaatt
     3361 gtaactcaga attttaaagc gatcattctg tccgtcgcca tttggtgaaa aaacattggg
     3421 aataaaaaga taaagcgaat cctctatcaa ttcctgagca taaacccaaa cagtatctac
     3481 tgcaatacaa ccattgggtg atgttgcaat aagtacaaca ctaaaagttc cggtttcaga
     3541 gaacgtttcc gaatatactg caccactacc cgatcctccg gtattgctta tccatgcgaa
     3601 gcttgcaccg ggagaatttg ttgctatgaa attgcctgca aggggaacat ttccggaaag
     3661 agaacttggt gaaatggacg caacaaccgt ttcaaccaga accaataccg gtccgtctgt
     3721 tactgtacca catgcattac ttacgctaac agaataatta ccggtagtgc taacagaaat
     3781 agaatttccg gtaactccat tactccaggt aagaactcct gttccggtag cttgcaaggt
     3841 tgctgactga ccactgcaca aaataattgt atctgaagaa gtaatttgaa cagttggaaa
     3901 ttccaaaaga gaaatgatca acgtatcaga aacactgcat ccggaaattg aaacaatata
     3961 tataacagta tcagagcctg aagcagccaa tgcgggatca tacaatcctg ttgctggatt
     4021 tgtaattgca gaacccgacc aaacaccacc cgatggcgtt gctgttagat ttaagacaaa
     4081 tgcgttttca cagatagctc cgggatcatt gatttgagca gctcccacat cgataacagt
     4141 tatgctttga actgaagatg ctgaacaccc attgccatct gtaaactggt aagtaataaa
     4201 atgtgtactg ctacctgctg ctgagggatc aaacattgtt ccgcttactc ctgtaccaga
     4261 aaacacacct ccgacgggag aagcaataag agacaatggc gaatcattaa tgcaaatgga
     4321 tggagacaac gaaaaggaaa caagcggatt ggaaaacact tctacttcca cactgtcgaa
     4381 aactgttccg caaacgtttg ttgagctcag ataaaccatt ccggaaacag aaacggaaat
     4441 ggaagaagca acagctcccg ttgaccaact gaaagatgaa actccaccgg cagataaatt
     4501 cactgcgccc ccgttgcaaa aacttaaaga tccatttacc agaatttctg gttgtggtaa
     4561 gggagtaagg tgaataagaa ctgagtcctt taccggaagg gaacatgttc cggaaaccag
     4621 aacatagaga tagaaattta ctgcatcaga tgcagaaaca atatatgaag cagacaagga
     4681 tgaagcattc ataaaaactc cgttaccccc ggtccattgt atcccttgag aaaaaccgtc
     4741 cactattgcg ttgatagtaa gtgtatcgcc aaaacaagca ttaatttgtg tcaatgaaat
     4801 gtcaagtgtt gtagaaacaa aaggcgccgt acacccctca ttgacataag tgggagtacc
     4861 taaaaaatta aaatcaactc ttgatccatc atttagggct gctgctccgc cataggttcc
     4921 ggattggttg actaaaagtg tacgatcgta ggcaacgata tctgaacaaa ccggacttga
     4981 aacaccaatg gaaagggttc gaaggctcga tgctccggtt ggtgctgcag tggcagttcc
     5041 tgaattattg tggttggcaa aatgtccctg gaaatttccc ggattctgaa agatcacata
     5101 cagcgtatcc tgtaacccgg caaaagaatt cgctccggta cacatatcgg tactggtgat
     5161 caataataca cgcttacctg caggaataat tccacccgga ggttccatca aacgtccaca
     5221 cggagattgt acagtagact gaagcgtagc aacaagaaaa gcagttgttg catttggtgt
     5281 tgctataccc aagaatgaat tgttcggcca ggttacggta aggcttccaa ttggaattgg
     5341 tgaagggccg acaatgaacc gtaccatttc gtttttccct tcgcaactgc aattcgaact
     5401 ggcagcagag gtacaccctg atcccggaac acaggcatca accagaatgc tttcaatttc
     5461 cagacattgt gttggaactt gtgcattaac tataccgaag aaaaaaatta tccaagaaaa
     5521 aaaaagagtc c
//
LOCUS       DAOMYG010000197         5530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5036_length_5530_cov_9.725114, whole genome shotgun sequence.
ACCESSION   DAOMYG010000197 DAOMYG010000000
VERSION     DAOMYG010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5036_length_5530_cov_9.725114"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="PK610_07535"
     CDS             <1..161
                     /locus_tag="PK610_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRJ35824.1"
                     /translation="ASGGAGSKSHFSDVFKNGKADAALAASVFHFGEISIPELKVYLQ
                     QNGISVRV"
     gene            171..764
                     /gene="hisIE"
                     /locus_tag="PK610_07540"
     CDS             171..764
                     /gene="hisIE"
                     /locus_tag="PK610_07540"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HRJ35825.1"
                     /translation="MKIDFEKENGLVPAIVQDAASGKVLMLGYMNQEAYNKTIEEKVV
                     TFFSRSKQRLWTKGETSGNKLQVVEIKSDCDSDALLIKAIPKGPTCHTGADTCWGETN
                     EDSLAFLRKLEMVIAERKSGNDESSYTASLFRKGINKIAQKVGEEAVEVVIEAKDSND
                     ELFLSESADLLFHYLILLQAKGFTLSDVTAVLASRHK"
     gene            803..1285
                     /locus_tag="PK610_07545"
     CDS             803..1285
                     /locus_tag="PK610_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRJ35826.1"
                     /translation="MVKSIIIAAAFQFSGNAIEVSTSVSSREILADSVVVYAKEFLGT
                     PYVWAGCSPKGFDCSGFVHYVFSKFGFSIGRSSSGLANAGSALAFEEADRGDLILFRG
                     TNPADKSVGHVGIVISPQGDSLQFIHASSSKKHFGVVITDYYRSGYPARFVAMRRMLQ
                     "
     gene            complement(1345..3222)
                     /locus_tag="PK610_07550"
     CDS             complement(1345..3222)
                     /locus_tag="PK610_07550"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ35827.1"
                     /translation="MRNFSFRVATICTTLLISNQVGAQCSLSITSTPASLNCGGGQVT
                     LQAVSSGGGNFALNNDFNLGNAGTGWTISPAGTFTNPCGPGLDGSTHMWMGSNTAAPR
                     TLQTAPLDVSCGGEICFDFRMSVQGDASPCEGPDLAGEGVYLEWSTDGGATWNTINYF
                     QPNTTGCFNATNNGGGSGCDGDYTAWANYCFPIPPIAQTSTTLFQWWQSGSSGNAFDH
                     WGIDNVTISSLVCSGYYYDWAHIPGFPDDSLITENVTQTTTFSVLYTNGIDDTCYATY
                     TVVVDTTIGVSTTPVAELCAGNNNGSVTTATNGGFAPYTYVLTGPSGSTNTTGSFTSL
                     APGTYNVAVTDNIGCFGSSTFTVTQGATVASTQNITPETCYLDNNASVQINSNGGSAP
                     YTYVLSGPVSGTNTTGSFNNLSAGNYTVNITDGNGCTASTTFTVNNTPDVVASFTSDY
                     AAGPAPLTVNFTNTSTGATTYNWSFGDGNTSTVVNPSNTYTTDSTFTVILVASNGPCA
                     DTTVLTLTTIQSIFFLPNVFSPNGDGVNSSFEFRTLNVKTLNCRIYNRWGTLVAELNS
                     PTAKWDGKDTDGKECPEGVYFYVLELTTVVAPIIYDPNPRQLTGTEFLFNGTVTVLRG
                     K"
     gene            complement(3291..4301)
                     /gene="metX"
                     /locus_tag="PK610_07555"
     CDS             complement(3291..4301)
                     /gene="metX"
                     /locus_tag="PK610_07555"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRJ35828.1"
                     /translation="MKQVFTYNLPLKLEYGGELPKVEITFHTYGEMSSTRDNVVWVFH
                     ALTANSDASDWWNGLIGEGKQFDPSKYFIVCANILGSCYGTTGPLSIDPSTRTPYYHR
                     FPFITIRDMVKAHRLLAKYLGVNKILFGLGGSMGGYQLLEWAAEESERFENIVLLATS
                     AAESAWGIAIHTTQRMAIEADPDWKENHPQAGKKGLMTARGIGMLTYRNYETYVRTQR
                     DEDGRIDGHSAESYIRYQGEKLARRFNAMSYYTLTKAMDSHHLGRERGSIESVLAQIQ
                     SNVLIIGISSDLLCPLAEQEFLARHIPHATYQLIDSSYGHDGFLIEFRAISDAISAHY
                     RS"
     gene            4401..4826
                     /locus_tag="PK610_07560"
     CDS             4401..4826
                     /locus_tag="PK610_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRJ35829.1"
                     /translation="MKIEIERRNSKFHFQAVNESGNTVDIDANPSIGGEGKGVRPMEL
                     LLAGLGSCSGIDIISILNKQKINPEKFSISVDGEREAGVVPSLFKIIRVTFSFEGEVD
                     AAKAKRAVELSMDKYCSVSKTLEPTAQIEYAIIVNGQRV"
     gene            4823..>5530
                     /locus_tag="PK610_07565"
     CDS             4823..>5530
                     /locus_tag="PK610_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRJ35830.1"
                     /translation="MKPKKQTEAIRTQVTVSGSKEHAVPMFLTSSFVFDDAEEMRAAF
                     ADEIERPIYSRFSNPNVDEFVEKMCKLEGTEAGYATASGMAAVFGTFAALLKSGDQIL
                     SCRSVFGATHTVFTKIFPKWNITAIYADIDKPESWESLLTPDVKMIYVETPTNPGVEI
                     IDLEWLGAFAKKHNLLLVVDNCFATPLLQQPKKFGADLIIHSATKFIDGQGRVMGGVV
                     LGPKNLIKEIYAFCRSTG"
BASE COUNT         1540 a         1120 c         1291 g         1579 t
ORIGIN      
        1 ttgcttccgg tggtgcaggc tcaaaatcgc atttttctga tgtttttaaa aatggaaagg
       61 cggatgctgc acttgctgcc agtgtgtttc acttcggtga aatttcaatc cccgaactaa
      121 aagtgtacct tcaacaaaat ggaatttccg tcagagtata agcgcaaaca atgaaaatcg
      181 atttcgaaaa agaaaatgga ctggttcctg caattgtcca ggatgctgca agtggcaagg
      241 tgctgatgct tggatatatg aatcaggaag cttacaataa aacgattgaa gaaaaggtgg
      301 tgacattttt ctcacggagt aagcaacgtt tgtggaccaa aggtgaaacg agcggaaaca
      361 aattgcaagt ggtagagatt aagtcggatt gcgattcgga tgcattgctc atcaaggcaa
      421 ttccgaaagg accaacctgc cataccggtg ccgatacttg ttggggagaa accaatgaag
      481 attctcttgc ttttttacgt aagctggaaa tggttatagc cgaacgtaaa tccggaaatg
      541 atgaatcgtc atataccgca tctttgtttc gaaagggaat aaataaaata gcacaaaaag
      601 taggggagga agctgttgag gtggttattg aagccaagga tagcaacgat gaattgtttc
      661 ttagtgaatc ggcagatctg ttgtttcatt accttatttt attacaggca aaaggcttta
      721 cactcagtga tgtaacagct gttttggcct cgaggcacaa atagtatttt ccttaaacac
      781 tgattaactg tatctttgtg ttatggttaa gtcaataatc atagctgcag cttttcaatt
      841 ttcaggaaat gctatagaag tttcaacgag tgtttcttcc cgcgaaatat tagctgatag
      901 tgttgttgta tacgcaaagg agtttctggg gactccatat gtatgggcag gttgttcacc
      961 gaagggattt gattgctcag gatttgtgca ttatgttttt tcaaagtttg gcttttcaat
     1021 tggaaggtct tcgtcaggac ttgcaaatgc cggatcagcc cttgctttcg aagaggcaga
     1081 tcgtggtgac ctgattcttt tcagaggaac caaccccgca gataaaagtg taggacatgt
     1141 tggaatagtg atcagccctc aaggcgattc acttcagttt attcacgcat catcttcaaa
     1201 aaaacatttc ggtgttgtca ttaccgatta ttaccgatcc ggctatcctg cccgatttgt
     1261 agcaatgcga agaatgttac aataaaaaac ctgcaacatc attgaagtgc tgcagggttt
     1321 ttgatttact ttctattccg aaaattactt ccctctcagt acagttactg ttccgttgaa
     1381 taggaattct gttccggtaa gctgacgtgg atttggatcg taaatgatag gtgctacaac
     1441 ggtagttaat tcgagcacat aaaaataaac gccttccgga cattcttttc cgtcggtgtc
     1501 ttttccgtcc cattttgcag taggtgaatt taattccgca acaagtgttc cccagcgatt
     1561 gtaaattcgg cagttaagcg tttttacatt aagtgtgcgg aattcgaaac ttgaattgac
     1621 accatctccg tttggagaga atacattcgg taaaaagaaa atcgactgaa tggtagtaag
     1681 cgttagaact gttgtatcag cacaaggccc gttggatgca accagaataa ccgtaaatgt
     1741 agaatctgtt gtgtaggtat tgcttgggtt aaccacagtt gaggtattcc catcgccaaa
     1801 cgaccaattg taagtagttg ctcctgtact ggtattagtg aagttcaccg ttaatggagc
     1861 cggaccagct gcgtaatccg aagtgaatga tgctaccaca tcaggagtgt tgtttacagt
     1921 aaacgtggtt gaggcagtac atccgtttcc atcggtaata ttcacagtgt aattacctgc
     1981 agaaagattg ttgaacgatc ctgttgtatt ggttcctgaa acaggtccgc ttaaaacata
     2041 ggtatacggt gccgaacctc cgttcgaatt gatttgtaca gaagcattgt tatcaagata
     2101 acaggtttcc ggtgtaatgt tttgggtgga ggcaactgtt gctccttgtg tcactgtaaa
     2161 tgttgatgag ccgaaacatc caatattatc tgtaaccgct acattatagg ttccgggtgc
     2221 aagtgatgta aaacttcctg tagtattcgt ggatcctgat ggtcctgtga gtacgtaggt
     2281 gtaaggtgca aatccaccgt tagttgctgt ggttacactt ccgttgttat tgcctgcaca
     2341 taattctgcg acaggagttg tgcttactcc aattgtagta tctaccacca ctgtgtaggt
     2401 agcatagcag gtatcatcaa taccgttggt atataaaacg ctgaaggtgg ttgtttgggt
     2461 gacgttttct gtaatcaacg aatcatctgg gaatccgggg atatgtgccc agtcgtaata
     2521 ataacctgaa caaacgagcg atgaaatggt aacattatcg attccccaat ggtcgaatgc
     2581 atttccggag cttccgcttt gccaccattg gaatagggtt gtagaagttt gcgcgatagg
     2641 aggaattgga aaacagtaat tggcccatgc agtataatcg ccatcacaac ccgatccacc
     2701 accattgttg gttgcgttaa aacatccggt tgtgttaggt tggaaatagt tgatggtatt
     2761 ccatgttgct ccgccatcgg tcgaccattc cagatacaca ccttcacctg ccaggtcggg
     2821 tccttcacaa ggggatgcat ctccctgaac ggacatgcga aaatcgaaac agatttctcc
     2881 tccgcaactt acatccagtg gtgcagtttg taatgtcctt ggtgcggcag tatttgatcc
     2941 catccacatg tgcgtgctgc catccaaacc agggccgcaa ggattggtaa atgttccggc
     3001 aggagaaatt gtccatcctg ttccggcatt tcccaaattg aaatcattat tgagtgcaaa
     3061 attacctcca cctgaagaaa cagcctgtaa tgtgacttgt cctccaccgc aatttaatga
     3121 tgcgggtgtt gaggtgatac tcaggctaca ttgtgcgcca acctgatttg aaatcaataa
     3181 ggtggtgcat atggttgcaa cccgaaatga gaaattcctc ataaagtgaa attagaatgt
     3241 gtagaataca aaaatcccca aaatgggaga agatttaccc catatctgat tcaggatctg
     3301 taatgagcgg aaattgcatc gcttatagcc ctgaattcaa tgaggaatcc atcgtgacca
     3361 tacgacgaat caatgagttg atacgttgca tggggaatgt gccgggccag aaattcctgt
     3421 tcagcaagag gacaaagcag atcactgcta atgccgatga tcagtacatt cgattgaatt
     3481 tgagccagca cgctttcaat ggatcctctt tctcttccga gatgatgact gtccatcgct
     3541 ttggtaagtg tataatacga catggcatta aatctccgcg caagtttttc gccctgatag
     3601 cggatatagg attctgcgct atgcccatca attctgccgt cttcatcgcg ctgggtacgc
     3661 acatacgttt cgtaattgcg gtaggtcagc ataccaatac cacgtgcagt cattaaacct
     3721 ttttttcctg cctgcggatg gttttctttc cagtcgggat cggcttcgat ggccattcgt
     3781 tgtgtagtat gaattgcaat tccccaggca gattccgcag ctgaggtggc cagcaaaacg
     3841 atgttttcaa aacgttccga ttcttcagca gcccattcca gtaattgata gccacccatt
     3901 gaaccaccca atccgaacaa aattttattt acaccaaggt attttgccag taaacgatgt
     3961 gcttttacca tatcgcggat ggtgataaat ggaaagcgat ggtaatacgg ggtgcgtgtt
     4021 gaaggatcga tactgagcgg gcctgttgtt ccgtaacacg atcctaaaat gttggcacaa
     4081 acaataaaat acttcgaagg atcaaactgt ttgccttctc caattaatcc gttccaccaa
     4141 tctgacgcat cgctgtttgc ggttaaggca tgaaataccc agacaacatt gtcgcgggtg
     4201 gaactcattt ctccataggt atgaaacgtg atttccactt tggggagttc acctccatat
     4261 tcgagtttta gcggaagatt atacgtaaaa acctgtttca tgtgctgggg caaatctagt
     4321 caaaatgata gattgtacag gaatctatcg ggcaataggg acatctcttt tcctatcgtt
     4381 gaagaacaaa gccccatgca atgaagattg aaatagagcg ccggaattcc aaattccatt
     4441 ttcaggccgt taacgaaagc ggtaatacag tggatattga tgccaatcct tcgataggag
     4501 gagaaggaaa aggtgtgcgt ccgatggagc tcttgcttgc aggattgggt tcatgcagtg
     4561 gaatcgatat catttccatt ctgaataagc aaaagatcaa tcccgaaaaa ttttccattt
     4621 cggtagatgg tgagcgtgaa gccggtgtgg ttccgtcctt gttcaaaatc attcgggtta
     4681 ccttttcttt cgaaggtgaa gttgatgccg caaaagcgaa acgtgctgtt gaattatcca
     4741 tggataagta ttgttccgtg tcgaagaccc tggaaccgac tgcgcaaatt gaatacgcaa
     4801 tcattgtaaa cggacaaagg gtatgaaacc aaaaaaacaa accgaggcaa tccgtacaca
     4861 agtaacggtt tcaggttcta aggaacacgc cgttccaatg tttctcacat ccagttttgt
     4921 atttgatgat gcggaagaaa tgcgcgctgc atttgcagat gagatcgaac gtccgatcta
     4981 ctcccgcttt tccaatccga acgttgatga gtttgtagaa aagatgtgta agctggaagg
     5041 tacagaagcg ggttacgcaa ctgcttcggg gatggctgct gtgtttggaa cattcgctgc
     5101 attattgaag agtggtgatc agatcctctc ctgccgttct gtttttggtg cgacgcatac
     5161 ggtgttcacc aaaatatttc cgaagtggaa cattacggct atttatgccg atatcgataa
     5221 accggagtcg tgggaatcgc tgctcactcc tgatgttaag atgatatatg tagaaacacc
     5281 aacgaatccg ggtgtggaaa ttattgatct ggagtggttg ggtgcattcg caaaaaaaca
     5341 caacttactt ctggtcgtgg ataattgttt tgcaacaccg cttcttcaac aacccaaaaa
     5401 atttggcgcc gatctgatca ttcattccgc tactaaattt atcgacggac aaggtcgcgt
     5461 aatgggtgga gtagtgcttg ggccaaaaaa tctgatcaag gaaatttacg ccttctgcag
     5521 aagtacaggt
//
LOCUS       DAOMYG010000198         5516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5062_length_5516_cov_9.317707, whole genome shotgun sequence.
ACCESSION   DAOMYG010000198 DAOMYG010000000
VERSION     DAOMYG010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5062_length_5516_cov_9.317707"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PK610_07570"
     CDS             <1..488
                     /locus_tag="PK610_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35831.1"
                     /translation="PFFLGWGVVHFQKMELRKSIKHKIIAGIDRGQLTLIKATPKNEH
                     LLHWHHSKEFEYQGEMYDIVEKSVSGDTTIYWCWWDNEETALNKTLNDLWLYALGKVP
                     NSNKQSQLLDFFFKSIYHDYSENNSAILFSNSAIKATYLNIRPTQNGFSTDFSPPPEA
                     A"
     gene            562..2991
                     /locus_tag="PK610_07575"
     CDS             562..2991
                     /locus_tag="PK610_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ35832.1"
                     /translation="MKRFVIAIACTCISLACSAQTIFVRDANNGRPVQGVVLSSKNPE
                     ASATTNEFGQADISALRGSDSILIRHFGYNVFIVSYAFLETKQFQISLSELSVTLKEV
                     IVESGRWRDLSLETPTRIERMNMQDAAFQNPQTAADLLGTSGYVFIQKSQLGGGSPML
                     RGFATNRVLLVVDGVRMNTAIFRAGNLQNVISLDANALQGVDVVFGAGSVVYGSDAIG
                     GVMDFRTLVPRFADSASKKPIVTGNVLMRSSSANSEKSGHFDFNTAFKKWAFTTSFTS
                     SEYGDLRSGSKGGDPYFYRPYYVETIDNKDYMVKNNDSTLQVGTNFSQINFMQKVSFK
                     ANNSLVFDYGFHFSETSKFNRYDRLYIMQTSGPYKNKLRWAEWFYGPQKWTMHRFGIT
                     LDKATRIYDKAQVIFAAQYFEESRNVREFMFRELLMQKENVKALSLNVDFEKRISPRL
                     SVNYGVEAVHNLVTSLATLTHVITLEEEPSVTRYPDGSTWESYSAFATGKYQLTEKIV
                     LNSGVRYNYYRIKASFDTTFFPFPFTNTVMQNGSISGSLGFVYSPNKTMQGYLNFASG
                     FRAPNIDDMGKVFESTPGYLVVPNPNLKPETVYNFEVGAVKTFGKYLRIDFAAYYTFL
                     FDGMIRKNFTFNGDTTIRYLGNKSRIQAVQNVSEIHVYGIQGGIEFNYKGFGITSNIS
                     YQSGKEQSPDSLIFYPLRHAAPLFGTTHFTYRYKTLKFDFYLIYNGRMDFEDLAITER
                     LNASYAKNELGQNYASSWYTLNLKAGYFINEYLGINIGVENLLDKMYRPYSSGLNAPG
                     RNFIVALRGRF"
     gene            3013..4452
                     /locus_tag="PK610_07580"
     CDS             3013..4452
                     /locus_tag="PK610_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ35833.1"
                     /translation="MKNYMNWSWLTVIIVLFSMNGYSQKIAAGGWHSLVVCTDSTVKA
                     FGENATGQLGNGNNTDQPSPVTPTGLPQIIAVSGGGDQLEAHSLALGANGKVYSWGSN
                     LYGGLGNGTLTNTNIPGQVLLLTDAIQISAGGWHSVALKADGTVWTWGWNIDGQIGDG
                     TFTDRILPTMVPGLTNVIQVVAGTYHNLALKNDGTVWAWGDNVSGQIGDGTTTDRTTP
                     VQVSNLSGVSKIAAGRFFSLAIKNDGTVWTWGENLYGQLGNGNTTDSHVPVQVSGLNG
                     ITTNIVAVGAFHCLIVKDDGTVWAWGRNTYGNLGDGTTTHRSTPVQTTGITDVVGMAA
                     GTNFSLLYKADGTFWGLGRNASGQLGDGTLLQRNTVNQSTLVCPIQHPVSTGNEIAIN
                     KVNLHCFPNPTFTGKINIVINNDDFYQVESGIISVHNTLGDCFLTLPLTKIEFPGQYE
                     LDLSNIPNGIYFIRLNLGEQTLIKKIIKL"
     gene            4462..>5516
                     /locus_tag="PK610_07585"
     CDS             4462..>5516
                     /locus_tag="PK610_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35834.1"
                     /translation="MKTIYIFFRSLAFRCQKMPDINCAIVFLMFAISANVNSQTISAG
                     WSHSSNLCGNNLYGWGANSFGQVGNGLLVDSYTPVQTFNTSGIRIISAGYRHTLMLRT
                     DSTVWAFGDNTYGQLGNGNNTSTPIAVKTNGLNQVISISGGKAGYHSLALKVDGTVWA
                     WGRNSEGQLGNGTTTDSNVPIQVLGLTDVIAIAGGEYHSLAIKSDGTVWAWGRNNEGQ
                     LGDGSNTNSNVPVQVLISDIIKISGGRYFSMALKNDGTVWTFGQNGHGQLGNGTTTNS
                     NTPVQVTGLTNVEHISGAAFHAMAILDDGSLYSWGRNDYGQLGDGTTTNSSLAIQIAG
                     ISNVIEIAGGTNYSLAKT"
BASE COUNT         1690 a         1066 c         1177 g         1583 t
ORIGIN      
        1 ctccgttttt tctgggttgg ggtgttgtac attttcaaaa aatggaactg cgtaaatcta
       61 ttaagcacaa aattattgca ggaattgacc gcggacaatt aacccttatt aaggcaacac
      121 caaaaaacga acatttactg cactggcatc attctaagga gtttgagtac caaggtgaaa
      181 tgtatgacat agtggaaaaa tcggtttctg gtgatactac tatttactgg tgctggtggg
      241 acaatgagga aaccgcatta aacaaaacgc tcaatgatct ctggctgtat gccttgggga
      301 aagtacctaa ttccaataag caatcgcagt tactggactt cttctttaaa tcaatttacc
      361 acgattattc agaaaacaac tctgcgattc tttttagtaa tagcgccatt aaagctacgt
      421 atttgaatat ccgccctaca caaaatgggt tttcgacaga tttttctccg ccgccggaag
      481 cagcataaag cctgttaatt tctgttttcc cttctattaa tttaagaaga ggaattttct
      541 attctttaag aattttcaat aatgaaacga tttgttattg caatcgcatg cacctgcatt
      601 tcattggcat gttctgcaca aactatattt gtgagagatg ccaataatgg caggcctgtt
      661 cagggtgttg tcctatccag caaaaatccg gaagcttcag caaccacaaa tgaatttggt
      721 caggctgata ttagtgcact cagaggttca gacagcatat taatccggca tttcggatac
      781 aatgtcttta ttgtaagcta tgcatttctt gaaactaaac agttccagat ctcactgtct
      841 gaattatctg taacactgaa ggaggttatt gttgaatccg gacgatggag ggatttatcg
      901 ctggaaacac ctaccaggat tgagcgtatg aatatgcagg atgctgcatt tcaaaacccg
      961 caaacagcag ccgatctgtt gggcacaagc ggatatgtct ttattcagaa aagtcaacta
     1021 ggcggtggtt cgcccatgct gcggggattt gcaacgaaca gggtattgct tgttgttgat
     1081 ggggttcgca tgaatacggc aatttttcgt gcaggaaatt tgcaaaatgt aatttccctc
     1141 gatgctaacg cattgcaggg tgtcgatgta gtttttggag ccggttctgt ggtttacggc
     1201 agtgatgcaa tcggcggggt aatggacttc aggactttgg ttccccgatt tgcagacagt
     1261 gcttcaaaaa agccaattgt taccggaaac gtcctgatga gaagctcttc cgccaattcc
     1321 gaaaaatcgg ggcatttcga ttttaatacc gcctttaaaa agtgggcatt tacaaccagc
     1381 ttcacttctt cggaatatgg agatctacga tcgggttcaa aaggtggcga tccgtatttc
     1441 tacagaccat actacgttga aaccatcgac aacaaagatt acatggtgaa aaacaatgat
     1501 tcaactttac aagtcggaac taatttcagc cagatcaatt tcatgcaaaa agtcagtttc
     1561 aaggcgaata attcgctggt gttcgactac ggatttcact tttcggaaac atcaaaattc
     1621 aacagatacg acaggcttta catcatgcaa acctccggcc cgtacaaaaa caaattacgt
     1681 tgggcagaat ggttttatgg tccgcaaaaa tggacaatgc accggtttgg tattacactt
     1741 gataaagcaa cccgcattta tgataaagca caggtgattt ttgccgcaca atattttgaa
     1801 gaaagccgaa acgtaagaga atttatgttc agggagcttc tgatgcagaa agagaatgta
     1861 aaagcactct cactgaatgt cgattttgaa aaacgtatca gtcctaggct ttctgtgaac
     1921 tatggcgtgg aagctgtgca caatcttgta acatcacttg caaccttaac tcacgtaatc
     1981 acgcttgaag aggaaccaag tgttacccga tatcccgatg gttcaacatg ggaatcatac
     2041 agtgcatttg caaccgggaa atatcaactg acggaaaaaa ttgttctgaa ttcaggtgtc
     2101 cgttataact actaccggat caaagcaagt tttgatacta cattttttcc gtttcccttt
     2161 accaataccg taatgcaaaa cggatcgatt agcggatccc ttggatttgt atacagtcca
     2221 aacaagacta tgcaaggtta tttgaatttt gcatccggat tccgcgctcc gaatattgat
     2281 gatatgggaa aagtatttga atcgactccc ggatacctgg tggtaccaaa tccgaatttg
     2341 aaaccggaaa cggtttacaa tttcgaagtt ggcgctgtaa aaacttttgg aaaatatttg
     2401 cgaatagact ttgctgccta ttacaccttt ttatttgatg gtatgatcag aaaaaatttc
     2461 acatttaatg gtgatactac tattcgatat cttggaaata aaagcaggat acaagctgtt
     2521 caaaatgttt ctgaaataca tgtttacgga atccaaggag gaattgaatt taattacaaa
     2581 ggatttggaa taacgagtaa catctcttac caaagtggta aagaacaaag tcctgacagt
     2641 ttgatttttt atccattacg ccatgctgca ccactttttg gtacaacgca ctttacgtat
     2701 aggtacaaga ctctgaaatt tgatttctac ttgatttata acggtcggat ggattttgaa
     2761 gatcttgcga tcacggaaag gttaaatgcc tcttatgcta aaaatgaatt gggtcagaac
     2821 tacgcatcat catggtatac actgaattta aaagctggtt attttatcaa tgaatatctg
     2881 ggaataaata ttggcgtgga aaacttactg gataaaatgt acaggcctta ctcatccgga
     2941 ttaaatgcac caggcagaaa cttcattgtt gcattacgtg gacgatttta aaaaaaacta
     3001 actcttttaa aaatgaaaaa ttatatgaat tggtcttggc ttactgtaat cattgtgctt
     3061 ttttcaatga acggttacag tcaaaaaatt gctgcaggtg gctggcattc ccttgtggtt
     3121 tgtaccgaca gcaccgttaa agcattcgga gagaatgcaa cgggtcagct gggtaacggc
     3181 aacaacactg atcaacccag tccggttact ccaaccgggc tccctcaaat cattgccgtc
     3241 tccggcggag gggatcagct cgaagcacat tctttagcat taggtgcaaa cgggaaagtc
     3301 tattcatggg gaagcaactt atatggaggc ttaggaaatg gaacacttac caataccaat
     3361 attcccggtc aagttttact tcttacagat gcgattcaga tttcagcagg aggatggcat
     3421 tctgttgcat taaaagcgga tggaactgta tggacatggg gatggaacat tgatggacaa
     3481 attggggacg gaacttttac cgacagaatt cttcctacaa tggtaccagg ccttacaaat
     3541 gtgattcaag ttgtagccgg aacgtatcac aacctcgctt taaaaaacga tggaactgtt
     3601 tgggcatggg gtgataatgt tagcgggcag attggggatg gaactacaac agatcggacc
     3661 actccggtac aagtgagcaa ccttagcgga gtttcaaaaa ttgctgcagg aagatttttt
     3721 tctcttgcca ttaaaaacga tggtaccgtc tggacatggg gcgagaatct ttacgggcaa
     3781 cttggtaacg gaaacacaac tgatagtcat gttccggttc aggtaagtgg attaaatgga
     3841 ataaccacca atatagtagc cgttggtgca tttcattgct tgattgtgaa agacgatggt
     3901 acagtttggg cctggggtag aaatacatat ggcaatctcg gtgacggaac tacaacgcat
     3961 cgaagcacgc cggtgcaaac aacagggatt acagatgttg ttggaatggc tgccggaaca
     4021 aacttctcgc ttttgtacaa agcagacggt acattttggg gcttaggcag aaatgccagt
     4081 ggacaattag gtgatggaac attacttcaa aggaataccg ttaatcaatc tacacttgtt
     4141 tgtcccattc aacatcctgt aagcaccgga aacgaaatag ccatcaataa agttaattta
     4201 cattgttttc caaacccaac cttcaccgga aaaatcaata ttgttatcaa taatgatgac
     4261 ttctaccaag ttgaatccgg aataatttct gtccataaca cccttggtga ttgctttcta
     4321 actttacctc tcaccaaaat tgagtttccc ggacaatacg aattagactt aagtaatatt
     4381 ccaaatggaa tttactttat tcgtttgaat ttgggtgagc aaactcttat caaaaaaatt
     4441 attaaactct aaaaaaatta catgaaaact atttatattt ttttccgttc gctggcattt
     4501 agatgtcaga aaatgccaga catcaattgc gctattgtgt ttcttatgtt tgcaatttct
     4561 gcaaatgtta attcacaaac tatttctgcc ggttggtcac actcttccaa tttatgcggc
     4621 aacaatttgt acggttgggg ggccaactca tttggacaag ttggaaatgg tctgttagtt
     4681 gattcctaca cgcctgttca aactttcaat acttcaggta ttcgcattat ctctgccgga
     4741 tacaggcaca cccttatgct gcgcaccgat agtacagttt gggcatttgg tgataatact
     4801 tatggacaac ttggaaacgg taacaatacc tcaactccaa ttgcagtaaa aacaaatggc
     4861 ttaaaccaag tgatttctat ttcaggaggt aaggcgggtt atcattcgct agctcttaaa
     4921 gtggatggaa ctgtttgggc atggggaaga aattcagagg gacaacttgg aaatggaact
     4981 acaactgaca gcaatgtgcc tatacaggtc ttaggcctaa cagatgttat agctattgcc
     5041 ggtggcgaat accattcttt agcaataaaa agcgatggaa cagtttgggc atggggaaga
     5101 aacaacgaag ggcaacttgg tgatggaagc aacacgaaca gcaatgttcc tgttcaagtt
     5161 ttaatttcag atattattaa aatctctggt ggccgctatt tttcaatggc acttaaaaat
     5221 gatggtacag tatggacttt tggtcaaaat ggccatggcc aacttggaaa tggcacaacc
     5281 accaatagca atacccctgt tcaagtaacc ggcctaacaa acgtagaaca catatccggg
     5341 gctgcatttc atgcgatggc aatattagat gatggctcac tttactcatg gggtagaaat
     5401 gactatggtc aattgggcga tggcactacc accaacagtt ctttagcaat acaaattgct
     5461 ggtatttcaa atgttattga aatagcaggt ggaacaaact actccttagc taagac
//
LOCUS       DAOMYG010000199         5510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5073_length_5510_cov_9.815582, whole genome shotgun sequence.
ACCESSION   DAOMYG010000199 DAOMYG010000000
VERSION     DAOMYG010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5073_length_5510_cov_9.815582"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..2356)
                     /locus_tag="PK610_07590"
     CDS             complement(89..2356)
                     /locus_tag="PK610_07590"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRJ35835.1"
                     /translation="MAFDIEMIKKVYAELPAKVDAARKMLGRPMTLAEKILYAHLAEN
                     LPSSPYERGKSYVDFNPDRVAMQDATAQMALLQFMQAGRPKVAVPSTVHCDHLITAKD
                     SSKADLDRAVKVNQEVYDFLSSVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTD
                     SHTVNAGGLGMIAIGVGGADACDVMAGLAWELKMPKLIGIKLTGKMNGWTSAKDVILK
                     VAGILTVKGGTDKVVEYFGPGAESLSCTGKGTICNMGAEIGATTSTFGYDASMARYLQ
                     STGRADVAELADKVKEHLTGDAEVYANPEKYFDEVIEINLSELEPYLNGPFTPDLATP
                     ISKMKEEAAKNGWPTKIEVGLIGSCTNSSYEDIARSASLANQAVAKNLKTKAQFNITP
                     GSELVRYTIERDGFIDTFNKIGANVFANACGPCIGMWDRAGAEKAEKNTIVHSFNRNF
                     AKRADGNPNTFAFVGSPELVTALAIAGDLSFNPLTDTLTNEKGEQVKLDPPSGNELPP
                     KGFAVEDAGYQAPAADGSKVNVLVSPTSDRLQLLDPFAAWEGVDLKGLKLLIKAKGKC
                     TTDHISMAGPWLKYRGHLDNISNNMLIGAVNAFNEKTDNVKNQLTGAYESVPKVQRAY
                     KAERIGSIVVGDENYGEGSSREHAAMEPRHLGVRAVLVKSFARIHETNLKKQGMLALT
                     FANKDDYNKIQEDDRFDITGLTSFAPGTPLTLVIHHSNGNSEEIKVNHSYNAQQIEWF
                     KAGGALNIIRASLKA"
     gene            2486..3631
                     /locus_tag="PK610_07595"
     CDS             2486..3631
                     /locus_tag="PK610_07595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ35836.1"
                     /translation="MSDRNRPVVLLFIDWYLPGFKAGGPIRSVANMVQHLGKDYDFRI
                     VTRNTDYTETTPYPTIESNTWVDGPFSEKVMYLSPEQCSKKGIQAILSGVNYDFVYIN
                     GIYSRIFSILPLELLRNEKKRKVIVASRGMLAPSAIAVKGFKKKLFLNWARLRGLYSN
                     VIFHATNATEERHIKDAIGKNALVQVADNFPALKVIPLSRKSGKESGVLRLVNIARIA
                     PEKNLEFALEMLRKVKDGKVRFEVYGPVYNEDYFNRCKSIAEQLPASCEVKFMGPVSP
                     EKITEILMRQDLLFMPTRGENFGHIILESLMAGTPVLTSDQTPFTNSGGVEALPLNDT
                     SAFAERLNEWLQMDEQTHQKKIEQSLEEARKKNDTFPLLERYRQLLG"
     gene            complement(3638..>5510)
                     /locus_tag="PK610_07600"
     CDS             complement(3638..>5510)
                     /locus_tag="PK610_07600"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRJ35837.1"
                     /translation="SEGNIKGYSEMLGSLLGTATKNRTKDQINKESDFIGASLNTSST
                     GIYGSSLKKHNEKLLELMSDILLNPVFNQDEMDKIKTQTLSGLQASKNTPESMAANVS
                     GFLNYGKNHPYGEITTEETVKNISLDDCKSYYETYFKPNVAYLAIVGDITPDEAKKLA
                     EKYFGGWRKGNVPQSPVPSVVFPKSTQLICVPRSNSVQSSVRITYPIDIKQSHPDALK
                     IKVLNEILGGGSSARLFRNLRETYNLTYGAYSSISTDQHIGNFNAFAEVRTAGTDSSV
                     NEFMKEIIRLRTEKVGQEELQGVINALTGKFAIGLEDPATIARFAINIDRYNLPKDYY
                     ENYLANLAKITADDVYEVAQKYLRPENAWIVVVGDRDKMPDLIARWKEKNALTFMDQY
                     GNPIIETRPAPSGLTAEKVIENYLNAIGGTANIKKIQSMVMRMSGELQGFKLEIVTKK
                     QNPKKKGLSKSSSSIILNGAMVVQKTVFDGAKGKQSGMAGEKMIEGEELEKMKLEAQP
                     HAELRYAELGYKLKLIGIDKINNKDAYKVEITDPKGEIEFTWYDVESGLAVKKEATQD
                     TPQGQVTSTTLMSDYREVNGVKFPYKIYQEVGPQIMDLTVTLIEINGKIDPKEFEVK"
BASE COUNT         1415 a         1190 c         1237 g         1668 t
ORIGIN      
        1 cttgatagga attgttactt gagaatcaaa aaagaacaaa aaaaaagaga gagaccaatg
       61 gcctctctcc tgaaatagga aattagaatt atgctttcag actcgcgcgg atgatgttaa
      121 gtgcgccacc ggccttgaac cactcaattt gttgagcgtt ataggagtgg tttaccttga
      181 tctcttcgct gttaccgtta ctgtggtgaa taacaagtgt aagcggagtt ccaggagcaa
      241 aggaagtgag cccggtaatg tcgaaacgat catcttcctg gatcttgttg taatcatcct
      301 tgttagcaaa agttagcgcc agcattccct gctttttaag attagtttcg tggatacgcg
      361 caaatgattt taccaataca gcacgaacac cgaggtgacg tggttccatt gctgcatgtt
      421 cacgcgaaga accttctccg tagttttcat cacctacaac aatagatcct attctttcag
      481 ctttgtatgc gcgctgaact ttcggtacac tttcgtaagc tccggtgagt tggtttttga
      541 cgttatcggt tttttcattg aatgcattca ccgcaccaat cagcatgttg ttggagatgt
      601 tgtcgaggtg accacggtat ttcaaccaag ggccggccat cgaaatatga tcggtggtac
      661 attttccttt tgctttgata aggagtttca atcctttcag atcaacacct tcccaggctg
      721 caaatggatc gagcaattgg agacggtcgg aagttggact taccagtaca ttcaccttcg
      781 aaccatcagc tgcaggagct tggtatccgg catcttcaac agcaaaacct ttgggaggta
      841 attcgtttcc ggatggagga tcgagtttta cctgttctcc tttttcgttg gtaagcgtat
      901 cggttaaggg attaaagctc agatcaccgg caatagccaa tgcagttacc aattcaggtg
      961 aaccaacaaa ggcaaaagta ttggggttac catctgcacg cttcgcaaaa ttgcggttga
     1021 aggagtgtac aatggtattt ttttctgctt tttctgctcc tgcacgatcc cacattccga
     1081 tacagggtcc acatgcgttt gcaaatacat tagcaccgat cttgttgaac gtatcaatga
     1141 atccgtcgcg ctcaatggtg taacgcacca attcagatcc tggcgtaatg ttaaattgtg
     1201 cttttgtctt tagattcttt gcaacggcct ggttggctaa tgatgctgaa cgtgcaatgt
     1261 cttcgtatga agagttggtg cagctaccaa tcaatcccac ttcaattttg gtaggccatc
     1321 cattctttgc agcttcctct ttcatcttag aaatgggagt agcaagatcc ggagtgaatg
     1381 gaccattcag atagggttcg agttcgctga ggttgatttc gattacttca tcaaaatatt
     1441 tttcaggatt ggcgtatact tctgcatcac ctgtaagatg ttctttcact ttgtctgcca
     1501 gttcagctac atcggcacga ccagtactct gaaggtaacg ggccatagag gcatcgtacc
     1561 cgaaagtaga tgttgtagct ccgatttctg cacccatatt acaaattgtt ccttttcctg
     1621 tgcaggagag tgattctgcg ccaggaccaa aatattcaac gactttatcg gttccacctt
     1681 taacggtgag aattcctgca actttaagaa tgacatcttt agcagaagtc catccgttca
     1741 tttttccggt cagcttaata ccaatcagtt ttggcatctt aagttcccat gccaatccgg
     1801 ccatcacgtc gcaggcatca gctccaccaa caccaatggc aatcatacct aatccacctg
     1861 cgttaaccgt atgcgaatcg gtaccaatca tcatcccgcc gggaaatgca tagttttcca
     1921 atacaacctg gtggatgata cctgctcccg gtttccagaa tccgatgccg tatttgttcg
     1981 atactgaaga aaggaaatca tacacttcct gattcacctt aactgcacgg tcgaggtcgg
     2041 ccttggagct gtcttttgct gtgatcaggt ggtcgcaatg cacggtagat ggaaccgcaa
     2101 ctttcggacg accggcttgc atgaactgaa gcaaggccat ctgggcagta gcatcctgca
     2161 tggctaccct gtcgggattg aaatcgacgt aagattttcc ccgctcgtat ggcgacgagg
     2221 ggaggttctc agcaaggtga gcataaagaa ttttctcggc gagggtcata ggacgcccca
     2281 gcatcttgcg tgcagcatct acttttgcag gtagttcagc atacaccttc ttgatcattt
     2341 caatgtcgaa agccatcgtg cagttgtttg gtttatgtgt aaaacgggta tgggcgaaaa
     2401 tacgaaaaaa agaattctgc aatagctaaa aacgggtatt cgatagaagt tcgtaatacg
     2461 tcggcggatt cgtactttcg cagggatgtc tgatcgtaac cgacctgtag ttttgctgtt
     2521 tattgactgg tatttgccgg gctttaaggc cggtggacct atccgttcgg tcgccaatat
     2581 ggttcagcat ttgggtaagg actatgattt tcgcatcgtt acccgaaata cagattacac
     2641 cgaaaccacg ccctatccca ctatcgaatc caatacctgg gtagacggac cattttcgga
     2701 gaaggtcatg tatttgtcgc ctgaacaatg cagcaaaaaa ggaatacagg ccatactttc
     2761 cggagttaat tatgattttg tttacatcaa cgggatttat tcccggattt tttccattct
     2821 gccattggaa ttgttacgca atgagaagaa aaggaaagtg attgttgcct ccagaggaat
     2881 gttggctcct tctgccattg cagtaaaggg attcaaaaag aaactgtttc tgaactgggc
     2941 ccgtttacgc ggattgtatt caaatgtcat ttttcacgcc accaatgcaa cggaagaacg
     3001 acacattaaa gacgctattg gaaaaaatgc gttggtacag gttgccgata attttcctgc
     3061 attaaaagtg attcctcttt cgcgaaaatc aggtaaggaa agcggtgtat tgagactggt
     3121 aaatattgca cggattgctc ctgagaaaaa tctggagttt gcactcgaaa tgctcaggaa
     3181 agtgaaagat ggaaaagttc gttttgaggt gtacggacct gtctataatg aggattattt
     3241 taatcgttgt aaatcgatag cggagcaatt acctgcatcc tgtgaagtga aatttatggg
     3301 acctgtttct ccagaaaaaa tcaccgagat cctgatgcga caggacctcc tgttcatgcc
     3361 tacccgcgga gagaatttcg gacatatcat tctcgaaagc ctgatggcag gaacacctgt
     3421 tttaactagc gatcagacac catttaccaa tagcggtgga gtagaagcac taccgttgaa
     3481 tgatacgtct gcattcgccg aaagattgaa tgaatggctg caaatggatg aacaaaccca
     3541 tcaaaaaaag attgaacagt cgttggaaga agcccgtaaa aaaaacgata ccttcccatt
     3601 gctggaaagg tatcgccaat tgttggggta atgactgtta tttgacttcg aattctttcg
     3661 ggtcgatttt cccgttgatt tcaataagtg taactgtgag atccatgatt tgcggaccta
     3721 cttcctgata gattttataa ggaaatttca cgccgtttac ttcacggtaa tcgctcatca
     3781 atgtagtaga agtaacctga ccttgtggtg tatcctgggt agcttctttt ttaacggcta
     3841 atccgctttc aacatcatac caggtaaatt caatttcacc tttaggatcg gtgatctcta
     3901 ctttataggc gtctttattg ttgatcttat caataccgat cagtttcagt ttgtaaccga
     3961 gttctgcata acgcaattca gcatgtggct gtgcttccag tttcattttt tccagttctt
     4021 caccttcaat cattttctct ccggccattc cgctttgttt gccttttgct ccgtcgaaaa
     4081 ctgttttttg cacaaccatg gcaccattca gaataatgct gctggatgat ttgctcaatc
     4141 cttttttctt gggattttgt tttttggtta cgatctccaa tttaaatcct tgcagctcgc
     4201 ccgacatgcg cataaccatg ctttgaatct ttttgatgtt ggcagttccg ccaatggcgt
     4261 tgaggtaatt ctcaattact ttttctgcag ttaatccgga aggagcggga cgcgtttcga
     4321 tgatgggatt tccatactga tccataaagg tcagcgcgtt tttctctttc cagcgggcaa
     4381 tgaggtcagg cattttatcg cgatcaccaa ctacaacgat ccatgcgttt tccggacgaa
     4441 ggtatttttg cgctacttcg taaacgtcgt cggcagtgat ctttgcaaga ttagccagat
     4501 agttttcgta ataatcctta ggtaaattat aacggtcgat attgatcgca aaacgtgcaa
     4561 tggttgcagg atcttcgagt ccgatcgcaa atttaccggt gagcgcattg atcacgccct
     4621 gaagttcttc ctgacccact ttttcagtgc gcagacggat gatttctttc atgaattcat
     4681 ttacggagct atcagttcct gctgtacgta cttctgcaaa tgcattgaag tttccaatgt
     4741 gctgatcggt gctgatgctt gaataggcac cataagtaag gttgtaggtc tcgcggagat
     4801 tgcggaacaa acgcgctgaa gaaccaccac caagaatttc attcagaact ttgatcttta
     4861 gtgcgtcggg atgactttgc ttaatatcaa tcgggtaggt gatgcgtacg ctcgactgaa
     4921 ctgagttcga acggggaaca caaatcagct gagttgattt cgggaatacc actgatggaa
     4981 cgggactttg tggtacgttt ccttttctcc atcctccaaa atatttttcc gccagttttt
     5041 tcgcttcgtc gggagtgata tcacctacaa ttgcaaggta ggctacattt ggtttgaagt
     5101 aggtttcgta atacgatttg caatcgtcga gcgaaatatt tttcaccgtt tcttccgttg
     5161 tgatttctcc gtaagggtga ttttttccgt agttcaaaaa accggaaaca ttggcagcca
     5221 tactttccgg ggtgttctta ctggcttgca gtccggatag ggtctgggtt ttgattttgt
     5281 ccatttcatc ctgattgaaa acagggttga gcaggatatc actcatcaat tcgagtagtt
     5341 tttcattgtg cttctttaag gaagaaccat aaattccggt tgatgaagtg ttcaatgaag
     5401 caccgataaa atcggattct ttattgatct gatctttggt tctgttttta gtggcagttc
     5461 caagtaagga tcccagcatt tcggaataac ctttaatgtt gccttccgac
//
LOCUS       DAOMYG010000200         5501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5091_length_5501_cov_7.924165, whole genome shotgun sequence.
ACCESSION   DAOMYG010000200 DAOMYG010000000
VERSION     DAOMYG010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5091_length_5501_cov_7.924165"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..2678)
                     /locus_tag="PK610_07605"
     CDS             complement(51..2678)
                     /locus_tag="PK610_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ35838.1"
                     /translation="MSKSNDPSFSKYKFRSLQCYCSTEWLSDSKKKYRSVFNRGETAY
                     IYAELCFYNKLFDEEEWNAQIRITCSFYEGDKESEICDIKLNRVVKTDENMAFIREGW
                     GNASYGMFWKKGTYLWRAYIDGELVGTHRFYVEDEQKVQSDQNVFFDVESLRFFEGDG
                     QAPAEADRVYYTSFHAANTRYVWGELKIRNKLPYPYHAEFFFNFYNDAGQLKGNSSEL
                     VYVKGEEFTVTSGWGSANKGTWFNDGYRLEISFMETLQAWAAFKVGDEFEEGIPNVLH
                     TITGQEKQEAVSGPKINEESLQQLLDKLDELVGMDNIKTKIRDYVKYLNFLKLREAKG
                     FKETQGISLHAVLTGNPGTGKTTIAKQLGKIYKEMGLLSKGHVHEVDRSDLVAEFIGQ
                     TAPKTKAAIEKARGGILFIDEAYSLFRNGEDNKDFGREVIEILLKEMSDGKGDLAVVV
                     AGYPQEMKGFLESNPGLKSRFNHFFHFEDYIPEEMMQIFELGLTQRSLTIADDGREFL
                     FTKFTQAYRDRDRSFGNARFVMSVVDEAKMNMGLRLMQHADIDALSESDLSTLSRDDI
                     YRIFAMGTTKSLDIRIDEKQLKEALDELNALVGLQNIKDDVMELVKLVKYYKEIGKDV
                     LNKFVLHSIFTGNPGTGKTTVARIFSKIFRALGILEKGHLIECDREGMVAGYSGQTAT
                     KTAHIIENAMGGVLFIDEAYALNQGRDDQFGMEAINTLLKRMEDNRDDFVVIAAGYTD
                     NMSEFLQTNPGLRSRFERVFQFKDYSPDELLLIADMMLKSEGLTAEEAARDHLGKYFL
                     TLWENRDKFFGNAREVRKVIGESIKNQHLRMAGVPASERNYEMIHTLTRADVEEFKVE
                     NIRSNAGSAKPVGFKSGER"
     gene            complement(2773..4026)
                     /locus_tag="PK610_07610"
     CDS             complement(2773..4026)
                     /locus_tag="PK610_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRJ35839.1"
                     /translation="MGETLHLELPFFANLLFLLVLARVLGEIFEHFRQPAMIGEITAG
                     IILGPSVLQITYFNEELKVFSEMAVFLLVIMAGLEINPKDIRGAMMGKKIWIAILGFV
                     IPIASGFLVGYLFQLDIMISIFLALCISITALPVSIRILIDLGKLHSDIGQKIIAVAI
                     FNDVIALMILGILIDIRGEASTFSEIASTTGVSLLKVLGFLLFVVIAYKIIDRATQKV
                     DFIKEQTDRLLNFLKGKESLFAIIFCFILVFASISESAGLHFIVGAFFGAMLITENLL
                     GKENFKQLEKTTTTITMGFLAPIFFAAIGLEFHIQSLNNIPLLLVVVAISFASKILGG
                     YLGARFSGMSHKGSLTIGIGLNARGIMELVIANIALQEGIIDIRLFSILVVMGLLTTM
                     STPILLKRAFAWIDKDKETAVPEVN"
     gene            complement(4113..>5501)
                     /locus_tag="PK610_07615"
     CDS             complement(4113..>5501)
                     /locus_tag="PK610_07615"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRJ35840.1"
                     /translation="ISGSNGLLRYTDQQNITKTTPLVSIRQFESANGSFRFSGSFGDS
                     TKIALQQLLIPELPYSDNSIGISFSSPTFDNTLPVLYSWKLVGYEEEWTEWTVTTLST
                     YTNLHEGRYTFLVRAKTPSGAESEIASFEFTILPPWYRTLWAYFLYAALFVLLIFISI
                     RIASYRLKQKNVQLEKIVSERTAEIAQKNTVLEHQNVQILHQKQEITDSITYAKRIQE
                     AILPIRMEIRKYFPDSFVLFKPKDIVSGDFYWFHHNDNISMVVCADCTGHGVPGAFMS
                     MICTDKLNHAVHEKNILQPGEMLSEVNRGIKRSLKQAGESHAQTKDGMDAAICAFHHA
                     SNTLTYSGANRPLWLIRNGTIEEYKPTKCAVGGFTSEDQIFEQISISIQAGDCVIMSS
                     DGYADQFGGEKGKKMMVKTFKEKLLEIATHPMSQQEQELDTYFENWKNNLTINGQVYE
                     QIDDVCVIGIRF"
BASE COUNT         1487 a         1238 c         1152 g         1624 t
ORIGIN      
        1 aggacaaaaa ccttaacttt aaacaacgag gattatgaaa aagtcaaagt ttacctttcc
       61 cccgatttaa agccaaccgg ttttgcgctt ccggcattgg agcggatgtt ttcaacttta
      121 aattcttcca catcggcacg ggtgagggta tggatcatct cataattgcg ttctgatgca
      181 ggtactcctg ccatgcggag gtgttggttt ttgatggatt ctccaatcac ttttcgtact
      241 tcacgtgcat tcccgaaaaa tttatcgcgg ttttcccaaa gcgtcaaaaa atatttcccc
      301 aaatgatccc gcgcggcctc ttctgcagta agtccttccg atttaagcat catgtcggca
      361 atgagtaaga gctcgtccgg actataatcc ttaaactgaa acacccgttc aaaacgcgat
      421 cgcaaaccgg gattggtctg caggaattca ctcatgttat cggtatatcc cgcagctatt
      481 actacaaagt catcgcgatt atcctccatg cgtttgagca gcgtgttgat ggcttccatg
      541 ccgaactgat cgtcgcgccc ctgattcaag gcataggctt cgtcgatgaa caatacgcct
      601 cccatggcat tctcgatgat gtgggctgtt ttggtggctg tttgccccga ataacctgct
      661 accattcctt cgcggtcgca ttcgatgagg tgacctttct ccagtattcc cagggcgcgg
      721 aagattttag aaaagatgcg tgcaaccgtt gttttacctg ttccggggtt tccggtgaaa
      781 atggaatgca gtacaaattt attcaatacg tcttttccga tttccttgta gtatttaacc
      841 agcttaacga gttccattac atcgtccttg atgttctgca atccaaccag cgcgttcaat
      901 tcatcaagcg cttccttcaa ttgcttttca tcaatgcgta tatcgagtga cttggttgtt
      961 cccattgcaa aaatgcggta gatgtcatcg cgactgagtg tactgagatc gctttcggat
     1021 aatgcatcga tatctgcatg ttgcattaat cgtaagccca tattcatttt tgcttcatct
     1081 accacactca ttacaaaacg tgcattacca aaacttctgt cgcgatcgcg gtaagcctgg
     1141 gtgaatttgg taaagagaaa ttccctgcca tcgtcggcaa tagtaaggga acgttgcgtt
     1201 aacccgagtt cgaagatctg catcatctct tcgggaatat agtcttcgaa atggaagaaa
     1261 tgattgaacc gcgatttcaa tccggggttg gattcaagaa atcctttcat ctcctgcgga
     1321 tatccggcaa caactacggc aagatcgcct tttccgtccg acatttcctt aagcagaatc
     1381 tcgatgactt ctcgaccgaa atctttattg tcttctccgt tgcggaagag cgaataagct
     1441 tcatcgataa ataatattcc gcctcttgct ttttcgatgg cagcttttgt ttttggcgct
     1501 gtttgtccga tgaattccgc aacaagatcc gagcgatcca cttcatgcac gtgacctttg
     1561 ctcaacaatc ccatttcctt gtagatctta cccaattgtt tggcgatggt tgtttttcct
     1621 gttcccggat ttccggttaa tacagcatgc agtgaaattc cctgtgtctc tttaaatcct
     1681 tttgcttcgc gcagttttaa aaagttcagg tatttgacgt aatcacggat ttttgttttg
     1741 atattatcca ttcccaccag ctcatccagt ttatccagaa gttgctgaag cgattcttca
     1801 tttatttttg gaccggaaac ggcttcttgt ttttcttgtc cggtaatggt gtgcaataca
     1861 ttcggaattc cttcttcaaa ttcatctccc actttaaatg ctgcccaggc ctgaagtgtt
     1921 tccataaatg aaatttcgag ccgatagcca tcattgaacc aggttccttt attggccgat
     1981 ccccatccgg aggttacagt gaattcttct ccttttacat aaaccagttc gcttgaattt
     2041 cctttcagct gaccggcatc gttgtaaaaa ttgaaaaaga attctgcatg ataagggtag
     2101 gggagtttgt tccggatttt taattcgccc catacatagc gtgtattcgc tgcgtgaaag
     2161 gaagtgtaat acacacgatc agcttctgca ggtgcttgtc cgtcgccttc gaaaaagcgg
     2221 aggctttcca catcgaaaaa gacgttttga tcggactgca ctttttgttc atcttccaca
     2281 tagaaacggt gcgttcccac taattctccg tcgatatatg cacgccataa ataggtgcct
     2341 tttttccaga acattccata ggaggcattt ccccatcctt cacgaataaa ggccatgttt
     2401 tcgtcggtct tcaccacacg attcaatttg atatcgcaga tttctgattc tttatctcct
     2461 tcgtaaaaac tgcatgttat tcggatctgt gcattccatt cttcttcatc gaaaagctta
     2521 ttgtaaaaac acaattcagc atagatataa gcggtctcgc cacgattgaa aacgctgcgg
     2581 tatttctttt tgctgtcgct tagccattcg gtagaacagt aacattgcag cgaacggaat
     2641 ttatatttgg agaaagaggg gtcgttggat ttgctcattg agatggactt tgttcccgaa
     2701 tataagcaaa aagtaaaaaa caggatcaga aatcagaagt aacagaagcg tatggaatac
     2761 ctgctttccg cattaattca cctcgggaac agctgtttcc ttgtccttgt cgatccatgc
     2821 gaatgcgcgc ttcagaagaa taggagtgct catggtggta agcaatccca tcaccacgag
     2881 gatcgagaat aagcggatat caatgattcc ttcctgtagg gcgatgttgg cgatgacaag
     2941 ctccataatt ccgcgggcat tcagaccaat gccgatggtg agtgatcctt tatgactcat
     3001 acctgaaaat cgtgcgccaa gataccctcc aagaattttt gatgcaaagg agatggcaac
     3061 cacaaccagt aacaatggaa tattattgag actttgaata tgaaactcca atccgatagc
     3121 tgcaaaaaag atgggagcaa ggaagcccat ggtaatggtg gttgtcgttt tttcaagttg
     3181 tttgaaattc tctttcccaa gcagattttc cgttatcagc atcgcaccaa agaatgcacc
     3241 aactataaaa tgcaatcctg cgctctcgga aatggatgca aataccagaa tgaaacaaaa
     3301 tatgatagcg aaaagcgatt cttttccctt gagaaaattc agcagtcgat cggtttgctc
     3361 cttaataaaa tctacttttt gagtagcccg gtcgataatt ttgtaagcga tgacaacaaa
     3421 caacaagaag cccaacactt tcagcaatga aactccggtg gtagaagcta tctctgaaaa
     3481 tgtgcttgct tctcctcgga tatcgatcaa aattccaagg atcatgagtg cgataacatc
     3541 attgaaaatt gcaactgcaa tgatcttttg accaatatcg gaatgtaatt tccccagatc
     3601 tatcaatatt cggatagaaa caggtagtgc tgtaattgaa atacacagag caaggaaaat
     3661 ggagatcatg atatccagtt ggaaaagata accaacaaga aatccggatg caatgggaat
     3721 gacgaaaccc agaatggcga tccatatttt tttccccatc attgcgccgc ggatgtcttt
     3781 tggattgatt tccaatccgg ccatgatcac aagaaggaat acggccatct cagaaaaaac
     3841 cttgagctct tcgttgaaat acgtgatctg taaaacggaa ggtccgagaa taattcccgc
     3901 cgtaatttca ccgatcattg cgggttgacg gaaatgttcg aagatctcac ccagcactct
     3961 ggcaagcacc agaagaaaaa gtaagttggc aaaaaagggt aactcaagat ggagtgtttc
     4021 gcccatggcc atggaataga gctcaaatat actccaattc acgaacgggg gtgaatttgt
     4081 cccgctattc tcttattatt ttacaggatg acttaaaaac ggatgccgat cacacagacg
     4141 tcatcaattt gttcgtaaac ctgtccgttg attgtcaggt tgtttttcca gttttcgaaa
     4201 taggtatcaa gctcctgctc ctgctgactc atagggtgtg tggcaatctc cagcagtttt
     4261 tccttgaagg tttttaccat catctttttc cctttttctc ctccaaactg atccgcataa
     4321 ccatcgctgc tcatgatcac acagtctccg gcctgaatgc taatggagat ctgctcaaaa
     4381 atctgatcct cggaagtgaa tcctccaact gcacatttag tgggtttgta ttcttcgatt
     4441 gtgccattgc gaatcaacca cagtggacgg ttcgctccgg aataggtgag tgtattgctc
     4501 gcgtgatgaa aggcgcaaat cgcagcatcc attccgtcct tggtttgtgc atgactttct
     4561 cctgcctgct tgagtgaacg cttgattcct ctgttcactt cactcaacat ttcaccaggt
     4621 tgcaagatgt ttttttcatg aacagcatga ttgagtttgt cggtacaaat catactcatg
     4681 aatgctcccg gaacaccatg accggtgcaa tcggcgcaaa caaccatgct gatattgtcg
     4741 ttgtgatgga accagtaaaa atcaccgctc acaatatctt ttggcttaaa caacacaaac
     4801 gaatccggaa aatattttcg gatctccata cgtatgggaa gaatcgcttc ctgaatacgc
     4861 ttggcatagg tgatggagtc ggtgatctct tgtttctgat gaagtatttg tacattctga
     4921 tgctccagga ccgtgttttt ttgtgcaatc tctgctgttc gttcgcttac aattttttcc
     4981 agctgcacgt ttttttgttt caagcggtag gatgctatcc ggatcgaaat gaaaatcaac
     5041 aaaacaaata atgccgcata caaaaaataa gcccagaggg tgcggtacca gggaggaagg
     5101 atggtgaatt caaaagaagc gatctcactt tctgctcccg atggagtttt agcacggact
     5161 aaaaaggtat atctgccttc atgaagattt gtataagtac tcagtgttgt aactgtccat
     5221 tccgtccatt cttcttcata acctaccagt ttccagctgt acaacaccgg taaagtatta
     5281 tcgaaagtgg gggaggaaaa cgaaatacca atggagttat cggaataggg caattcagga
     5341 ataagaagtt gttgcagcgc aatttttgtg gaatctccaa agcttcccga aaaacggaaa
     5401 cttccgttgg ctgattcgaa ttgacgaatg ctgaccaggg gtgttgtttt ggttatattt
     5461 tgctgatccg tataccgcaa taatccgttc gatccggaaa t
//
LOCUS       DAOMYG010000201         5485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5115_length_5485_cov_8.547514, whole genome shotgun sequence.
ACCESSION   DAOMYG010000201 DAOMYG010000000
VERSION     DAOMYG010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5115_length_5485_cov_8.547514"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="PK610_07620"
     CDS             <1..1091
                     /locus_tag="PK610_07620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRJ35841.1"
                     /translation="MGSIIQSTPLILTLRKNYPNAKIIFITTSGNKRLLELIPQVDEI
                     ITLNDSSFFALLFGAPSLIAKLIRKRIGLYFDLEIYSNFSSAITAMSCARNRVGFYLR
                     SDQYRLGLYTHMMFYNVNMPIRETYLQMARILPLKNENSELHNLCEETSRLRPEIITS
                     SIHSKNHIVINCNASDLREERKWPLDRFAGLINYILNNHTFTIYLIGASNEKAHVAEL
                     MKRIPENFRIINLAGKTNIDELIALIKHSQMMITNDTGPMHIAYSTSTPTLALFGPCS
                     PLQYGTFPNSIALYESVYCSPCVHEFLVPPCKGNNICMQKIDLEKAIHAFEQILNGVP
                     QAQNTNYNAITGRENGYLPGLVKRQKNE"
     gene            complement(1095..2909)
                     /locus_tag="PK610_07625"
     CDS             complement(1095..2909)
                     /locus_tag="PK610_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ35842.1"
                     /translation="MRLIVFLFLLLPFFASSQGSDQQLAVQYFQAGEFDKAALYYEKL
                     YNKNQSDYFYNYYLKCLLELKEFKEAEKLVKKQQKKNQGNLKYFVDLGDVYKQQGDVA
                     KAKKEYDNAIKDLTGDVQQVRNLALSFRDKGELDYALKVYEKGEKLNGGAVKFRADKA
                     EIYNLKGETQLMIEEYLAMLEDNPRDLDRVENILTFAVNMDDPNNARVELLRTEILKR
                     VQKNPDNVAYNELLIWFFQQKGDLNAAFNQVKALDKRRKEDGLRVLSFGAVCANNQQY
                     DLAARAFEYVITEKGKNGNYYIDARIQMVEVLSYKILNQGIYEQNDLTTLERLYNESI
                     TELGRNSGTVGLMRGYAKLKAFYMQDPKGGTEILFEALQMPGIRPNIEAEMKLELADI
                     ALMRGLIWDASLLYSQVEKAFKHEPIGHEAKLRNARISFYTGDFGWAQGQLDVLKAST
                     SKLISNDAMQLSLLITDNLGVDSIPEALLLYAEADLLVFQNRLAEAELKLDTLNKQYT
                     WHGLSDESVYLRYKIAMKRRDYTTAVSHLQNIIENYGSDILGDDATFKLAELYQFQFN
                     DSEKASELYKKLMFEYPGSLYVVEARKRFRILRGEKIN"
     gene            complement(2918..3460)
                     /locus_tag="PK610_07630"
     CDS             complement(2918..3460)
                     /locus_tag="PK610_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35843.1"
                     /translation="MKYRIVPFLIFILFTAFFSSCGGPDFSTEIRSIDSLRVEIDRIE
                     KELGTLDSAILDTISMHASNNVNILKKVYDTDTIDMVRARKITTYKSLRRVNLRANKQ
                     RAELWAEIHLSVKQLENLKKSLEHGDFELEEGRKYFQEEKLATRALFSAYDAYKGSYE
                     STRQLYDSLNPYVLEVIRGQ"
     gene            complement(3486..4751)
                     /gene="serS"
                     /locus_tag="PK610_07635"
     CDS             complement(3486..4751)
                     /gene="serS"
                     /locus_tag="PK610_07635"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRJ35844.1"
                     /translation="MLEISYLRENKKEAIARLKKRNYDFTSVVEQILQIDADRRRTQN
                     ELDSQLAEMNRISKEVGALMQQGKKDEANVAKEKTAELKNSTKQLQEKLTEFEAQQQQ
                     LLYTVPNTPSAIVPEGKTPEENLEVFRHGTIPVLKDAVPHWELAAKHDLIDFELGVKI
                     TGAGFPVYKGKGARLQRALINFFLDEATKAGYAETQVPHLVNEASGYGTGQLPDKEGQ
                     MYHAGIDNLYLIPTAEVPLTNIYRDVILKEEEMPVKLTGYTPCFRREAGSYGKDVRGL
                     NRLHQFDKVEIVQLAHPDDSYRILEEMVEHVKGILMKLGLHFRIIRLCGGDMGFTSAL
                     TYDFEVYSAAQEKWLEVSSTSNFETFQANRLKLRFKDKSGKTRLAHTLNGSALALPRI
                     VAALLENNQSAEGIRIPEVLVPYTGFDKI"
     gene            complement(4891..5148)
                     /gene="rpmA"
                     /locus_tag="PK610_07640"
     CDS             complement(4891..5148)
                     /gene="rpmA"
                     /locus_tag="PK610_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRJ35845.1"
                     /translation="MAHKKGVGSSKNGRESESKRLGIKVFGGQKAIAGNIIVRQRCTK
                     HHPGENVGIGKDHTLFALVDGTVVFQKKKNDRSYVSIEPAN"
     gene            complement(5182..5484)
                     /gene="rplU"
                     /locus_tag="PK610_07645"
     CDS             complement(5182..5484)
                     /gene="rplU"
                     /locus_tag="PK610_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009388776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRJ35846.1"
                     /translation="MVEIAGQQFKVAKDQRVYVHRLPGEEGSKVEFDKVLLVDNGGKI
                     SVGAPAVEGAKVTGKILKHLQDDKVVIFKKKRRKGYKVKRGHRQQFTQIQISDIKA"
BASE COUNT         1534 a         1225 c          996 g         1730 t
ORIGIN      
        1 ggatgggttc tattatccag agcacaccgc tgatcctaac acttcggaaa aattatccga
       61 atgcaaaaat tatttttata acaacgtccg gaaacaaacg attacttgaa ctgattccac
      121 aagttgatga aatcataacc ctcaacgaca gttctttttt cgcacttctt tttggggctc
      181 cttcactaat tgcaaaactg attcgcaaaa ggatcggttt atatttcgat ctggaaatct
      241 attccaattt cagcagcgca ataacagcga tgtcgtgtgc cagaaatcgt gttggttttt
      301 atcttcggtc tgatcaatac cgattggggc tatataccca tatgatgttt tataatgtta
      361 acatgcccat tcgagagacc tatctgcaaa tggctcggat tcttccacta aaaaatgaga
      421 attcggaatt acataatctt tgtgaagaaa catccagact gagacctgaa attattacgt
      481 catcgattca ttcaaaaaat catattgtaa tcaattgcaa cgcttccgat ctgcgtgaag
      541 aacgcaaatg gccattggat cgttttgcag gattgatcaa ttatattctc aacaatcata
      601 cattcaccat ttacctgata ggagccagca atgaaaaagc acacgttgct gaacttatga
      661 aacgaattcc ggaaaacttc cgaatcatta atcttgccgg taaaacaaac attgatgaat
      721 taattgcgct aataaagcat tctcaaatga tgattaccaa tgatacgggt cccatgcata
      781 ttgcctattc tacttcaacc ccaacactcg cattgtttgg tccatgctct ccacttcaat
      841 acggtacatt ccctaattcc atcgctttgt acgagtcggt ttattgcagt ccctgtgtac
      901 atgaattttt agttccgccc tgcaaaggca ataacatttg tatgcaaaaa attgatcttg
      961 aaaaagcaat acatgcattc gagcaaatcc ttaatggtgt tccgcaagct caaaacacta
     1021 attacaatgc cattaccgga agagaaaacg gatatttgcc aggattggta aagcgacaga
     1081 aaaatgaata aggatcaatt gatcttttca ccccttaaaa tccggaaccg tttacgtgct
     1141 tccaccacat ataagctacc ggggtattca aacatcagct ttttgtaaag ttcagacgcc
     1201 ttttccgaat cattaaattg aaactgatac aattccgcaa gtttaaacgt tgcgtcatcc
     1261 cctaaaatat cacttccgta attttcaatt atgttttgca aatgggatac tgcagttgta
     1321 taatctctgc gtttcattgc aattttataa cgcaggtaaa cagattcatc tgaaagtccg
     1381 tgccaggtgt attgtttatt cagcgtatca agtttcagtt cagcttctgc aagacggttc
     1441 tggaaaacca ataaatcagc ctccgcatag agtaacaatg cttcaggaat tgaatcaacc
     1501 cccagattat cggtgatcaa taaagacaat tgcattgcgt cgttgctgat gagtttagat
     1561 gtagatgcct tgagaacatc aagctgcccc tgtgcccaac caaaatctcc ggtgtaaaat
     1621 gaaattcgtg cgtttcgcaa ctttgcttcg tgacctattg gttcgtgttt aaatgctttt
     1681 tctacctgtg aatagagtaa cgaagcgtcc caaatcaatc cgcgcataag tgcaatatcg
     1741 gcaagttcga gtttcatttc agcctcaata ttgggacgta tccctggcat ttgaagtgct
     1801 tcaaaaagaa tttccgtacc gccctttgga tcttgcatat aaaacgcctt caacttagca
     1861 tatcctcgca tcaatccaac agttccagaa tttctcccaa gttctgttat cgattcgttg
     1921 tacaaacgct ccaatgtggt aagatcattc tgctcataaa taccttgatt gagaatttta
     1981 tatgacaaca cttccaccat ttgtatacgc gcatcgatat aataatttcc gttttttcct
     2041 ttttcggtga ttacatattc aaatgcacga gctgcaagat catattgttg gttattggca
     2101 caaaccgctc cgaaactcaa aacgcgcagt ccgtcttctt tacgacgttt atctaatgct
     2161 tttacctggt tgaaagctgc atttaaatct cctttttgct gaaaaaacca aatgagcaat
     2221 tcattataag caacattatc aggattcttc tgaacacgtt taagtatttc agtgcgcaac
     2281 aactctaccc ttgcgttatt ggggtcatcc atgttaaccg caaacgtgag aatattctca
     2341 acacgatcaa ggtcacgcgg attatcttcc agcatagcaa gatattcttc aatcatgagc
     2401 tgagtctcac ctttcagatt gtaaatttct gccttgtcgg ctctgaattt tacggcgcct
     2461 ccatttaact tctctccttt ctcatatacc ttcaaagcat aatccaattc acctttatct
     2521 ctgaatgaaa gtgccagatt acgtacttgc tgaacatcgc ctgttagatc ttttatcgca
     2581 ttatcatatt ccttttttgc ttttgcgaca tctccctgtt gtttgtaaac atcacccaga
     2641 tcaacgaaat acttcaaatt tccctgattc tttttctgct gctttttaac cagtttctct
     2701 gcttctttga attcttttaa ttcaagcagg cacttcagat aataattgta aaaataatcg
     2761 ctctggtttt tgttatacag cttttcgtaa taaagcgctg ccttatcaaa ttcgcccgct
     2821 tgaaaatact gcacagccaa ttgctgatca cttccctgcg aagaagcaaa aaaaggtagt
     2881 agcaggaaaa gaaatacaat cagtcgcatt tatgagttta ttgtcctcga attacttcta
     2941 aaacgtaggg attaagagaa tcgtataatt gtcgggtact ttcgtagcta cccttatagg
     3001 catcatacgc agagaaaagg gcccgggttg ccagtttttc ttcctggaag tatttccttc
     3061 cctcttccaa ttcaaaatca ccatgttcca gcgacttttt taaattctca agctgtttca
     3121 cggacagatg aatttctgcc caaagctcgg cacgctgttt atttgcacgg aggttaactc
     3181 tacgcaatga tttataggta gtaattttcc gggcacgaac catatcaatg gtatctgtat
     3241 cgtacacttt cttaagaata ttcacattgt tactggcatg catactgatg gtatccagta
     3301 tcgcggaatc caaggttcca agctcttttt cgatccgatc tatttcaact cgtaaactat
     3361 caatactgcg aatctccgtg gaaaaatcag ggcctccaca ggaactaaaa aaagcagtga
     3421 ataaaatgaa gataaggaaa ggaactattc tgtatttcat atgcttttca ttttgaccgt
     3481 taaaatcaaa tcttatcaaa acctgtgtat ggaacaagta cttccggaat acgaattcct
     3541 tccgcgcttt gattattttc aaggagagca gcaacaatac gtggcaatgc taatgcagat
     3601 ccattgaggg tatgtgcaag acgtgtcttt ccacttttat ctttaaatcg aagctttaaa
     3661 cgattagcct ggaaagtctc gaagtttgag gtagaagaaa cctcaagcca tttttcctgt
     3721 gctgctgagt atacttcaaa atcgtaagtc aatgccgagg tgaaacccat atctcctccg
     3781 cataaccgaa tgatacgaaa atgtaaaccc aacttcatta atatcccttt gacatgttct
     3841 accatctcct ctaaaatccg gtaagaatca tcaggatgtg caagctgaac tatctcaact
     3901 ttatcaaatt ggtgcaaacg attcaatcct cgtacatctt tgccgtagct tccggcttca
     3961 cgacgaaaac atggtgtgta tccggttagt tttaccggca tttcttcttc tttaaggatt
     4021 acatcgcggt aaatatttgt aagcggaact tcagcagttg gaatgaggta taaattatca
     4081 attcctgcgt ggtacatctg tccctcttta tcgggtaatt gcccggtacc gtatcctgaa
     4141 gcttcgttta caaggtgcgg aacctgggtt tcagcataac cagcttttgt tgcttcatcc
     4201 agaaagaaat tgatcagcgc acgctgaagt cgggcgcctt ttcccttata aaccggaaag
     4261 cctgcacctg taattttcac gcccaactcg aaatcgatca gatcatgttt tgcagcaagc
     4321 tcccaatgag gaactgcatc tttaagtaca ggaattgttc cgtgacggaa aacttcaaga
     4381 ttttcctccg gtgtttttcc ttccggaaca attgcactcg gagtattagg tactgtataa
     4441 agcaattgtt gttgttgtgc ttcaaactca gtgagctttt cctgcaactg tttggtgctg
     4501 ttttttaatt cagctgtttt ctctttagca acatttgctt catccttttt cccctgctgc
     4561 atcaaggccc caacttcttt tgaaattcga ttcatttcag caagttgcga gtcgagttca
     4621 ttttgcgttc tacgacgatc cgcatcgatt tgcagaatct gctcaacaac agaagtaaaa
     4681 tcgtaattgc gctttttcag acgggcaatg gcctctttct tgttttcgcg gagataagag
     4741 atctcgagca tggtgtgttt tttttgtaaa aatagttgaa ttaacggtaa accttacaaa
     4801 tgacagttgc attcctgagg caaccccatt gagcaaaaaa acaaaacccc atccggatcg
     4861 gacagggttt cgaattcggt tgatatgcga ttagttcgca ggctcgatag aaacgtatga
     4921 acggtcgttc ttcttcttct gaaaaacaac tgttccgtct accaatgcaa aaagagtgtg
     4981 gtcttttccg ataccaacat tttcaccggg atggtgtttg gtacagcgct gacgaacgat
     5041 gatatttcct gcgatggcct tttgaccacc aaaaacttta atgccgagtc gtttggattc
     5101 cgattcacga ccgttcttgg aactacctac gccttttttg tgtgccatgg ctctacggtt
     5161 tttttaaggt tctacttacg gttaagcttt gatgtccgaa atctggatct gagtaaattg
     5221 ctgacggtgt ccgcgcttta ccttgtatcc ttttcttctt ttctttttga agatgacaac
     5281 cttgtcatct tgcaggtgct tcaggatctt gcctgttact tttgcacctt ctacagccgg
     5341 ggcgccaacg ctgatttttc caccattgtc aacgaggaga actttatcga actccacttt
     5401 tgaaccttcc tcacctggga ggcggtgtac ataaacgcgc tgatcttttg caaccttaaa
     5461 ttgctgccct gctatctcta cgatt
//
LOCUS       DAOMYG010000202         5461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5152_length_5461_cov_7.661487, whole genome shotgun sequence.
ACCESSION   DAOMYG010000202 DAOMYG010000000
VERSION     DAOMYG010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5152_length_5461_cov_7.661487"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1236
                     /locus_tag="PK610_07650"
     CDS             229..1236
                     /locus_tag="PK610_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRJ35847.1"
                     /translation="MPANKEAYIRYRIIDSCLRNKQRPFPTLGELAEKCEEVLGKTFS
                     ESTIQKDIYAMRFDQGLGFEAPIEYSKLHKGYSYTDESYSISSIPLKEEELVAIEFAA
                     GILQQFTGVGLMGKFDEAITKIMESVNISRAMGEDNSSRIVQVEHHHTEIDPSLLETI
                     TQCIINKKVIRFNYHSFGSGKRSVKTLHPYLLKEYRNRWYVIGWSSGDKDMRTFGLER
                     ITEAELIKEKYYRHTDFDPDAYFKYSFGITVGDNKPEEIILEFKGYEKLYVKSQPLHS
                     TQKTIKESDQSLVIALQVIPSPELTMAIRSYGAEVKVIKPAWLAKEIKSGLQSALKNY
                     E"
     gene            1233..2273
                     /locus_tag="PK610_07655"
     CDS             1233..2273
                     /locus_tag="PK610_07655"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRJ35848.1"
                     /translation="MNNTEKIDRIANKQVDGRYIHGISLCIQNESRDLDHLISLGNLT
                     DDHPYFIASTTKLFTTAIILQLRNENKLRLEDPIGLFLSADQLAGLHQLKGIDSISKI
                     TIKHLLAQTSGIGDYFSGKPKGGVSLEKELMEGKDRYWSFEEAMDRARTISPAFFPGT
                     NGKALYSDTNFQLLGKIIENITGHSYAQAVDLRISKVLGLKTTYVYSDETDRTPLCLY
                     YKRNPLSIYKAMSSVQVDGGIVSNATESMVFLRAFFNGQLFPKSYLEELYSWNKIFFP
                     LQYGVGITRFKVPWFFSPFKSIPEIIGHSGLSGAFAYYCPAKGLYLTGTVNQIHSPSV
                     SYQMLIRILNLF"
     gene            2455..4443
                     /locus_tag="PK610_07660"
     CDS             2455..4443
                     /locus_tag="PK610_07660"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:NF037004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HRJ35849.1"
                     /translation="MLRMISTYHIQPQESNDAFSEKVMNRLIRRIDPIGLLFLEEEVK
                     DLRGFSSRLDDEWETENCGFIRLLQESYSKAINRAERIIQNLLETPFNYNEKESIQFY
                     TSENQFGKDENELKIKWKRFLKLEILDILFIRDPESGKIPTVAEALKNEKDARQKVKK
                     KEAKRIARKKEYPGGIENYVMEIYLDCIATAFDPHTQFFSERINNQFKESLSSETLSF
                     GIDWTEAKDGNPEVAEMNPGGPAWNSGEINIGDQVIFVEWQDGTTTSSNDLDVEDFIR
                     KSELATTTKITLTFQKSDGQVRKVSLQKEKMRSEENIISGFILEEKKRIGYITLPAFY
                     TQWETINPKGCAQDVAREIIKMKQENIEGLIIDLRFNGGGSMYEALQLAGIFIDEGAL
                     GIQKYSQTKPQIFKDPNRGTVYTGPLLILVNRHSASASEVFAAAMQDYNRAIIAGTTT
                     YGKATGQAVIPLDTTAQTKKNTVNVGPADFVKITMLALYRCTAKTHQARGIVPDIEIP
                     DISNYSSGGESEEETHLKLDSVDKKSYFKPFPSKDYTALREKSQQRLASDSLMIWLKK
                     HEKAIIQYYNENQVIDLDINTYYSNSNLERKIFEDYYRITGIPSEEIRFYLHAYDRDL
                     ISIDSFRKSIFENQMENLKMDRQLREAFYILSDYIQSQ"
     gene            4460..>5461
                     /locus_tag="PK610_07665"
     CDS             4460..>5461
                     /locus_tag="PK610_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35850.1"
                     /translation="MKAICTLLIFISGMGMINAQKFTNAGEYMGFIQKQYQEISKDTW
                     SYISAVAHGKSARKVEKRRMEVITTTQKALNTIKGLQPFDGDKTLRDSAVSYLTLSLN
                     VVREDYGKIMDLEAIAEQSFDAMEAYLLAQEKAGEKMDQAGEMMEVVTKAFAEKHGVT
                     LIESKDQLGKKMEIASEVIKYYNQIYLVFFKSYKQEAYMLDAVQKADVNGVEQNKTSL
                     NQFAKEGLEKLKEFKPYNNDGSIIAACNELLKFYVMETEEKISSITDFLIKKENFDKK
                     NAAFQAKKEKDRTQADIDQINAAGKEYNEAVNNYNKTNTELFNKRKALLDNWNKTVQT
                     "
BASE COUNT         1855 a         1111 c         1116 g         1379 t
ORIGIN      
        1 agtaattttt tcatgtctga tacagatttc agacagcaaa atctaaaaaa aaaaaatgaa
       61 ttcgcaaaat agttgtcagg tattttgcga taaaatcagt caattattac atctctagct
      121 ctcttcaatc gcttcactgc cgcgattgtt caaactataa gtatttaact gcgaggctaa
      181 gtaggctgtg caactgctcc acttttgcta tctttacaaa aaacctccat gccggccaat
      241 aaagaagcgt atatccgtta ccgaatcatc gattcctgtt tgcgcaacaa acaacgtcct
      301 ttccctaccc ttggtgagct tgcagaaaaa tgcgaggaag tgctgggtaa aacattttcg
      361 gaatcgacca tccaaaaaga catttacgcc atgcgcttcg atcagggtct tggatttgaa
      421 gctccgatcg aatacagcaa actgcacaaa ggctattcct ataccgacga aagctattcc
      481 atttcgagta ttccgctgaa agaagaagaa ctggttgcaa ttgaatttgc agcgggaata
      541 ctgcaacaat ttaccggggt tggactaatg gggaaattcg acgaggccat tacgaagatc
      601 atggaatcgg taaatatttc gagagcaatg ggcgaagaca attcaagccg tatagttcag
      661 gtagagcacc accacaccga aatagatcct tcgttactgg agaccattac ccaatgcatc
      721 atcaataaaa aagtcattcg tttcaactac cactctttcg gcagcggaaa acgttcggtg
      781 aaaacactgc atccttacct gctgaaagaa taccgcaacc gctggtatgt gattggctgg
      841 agttccggtg ataaggatat gcgtacattc ggtctggaac gaataacaga agctgaattg
      901 atcaaggaaa aatactaccg acataccgat tttgatccgg acgcttactt taaatattcg
      961 ttcggaataa cagtgggcga caacaaaccc gaagaaatta ttctggaatt taaaggctac
     1021 gaaaaactat acgttaaatc acaaccactc cacagcaccc aaaaaacgat caaagaaagc
     1081 gatcagagtc ttgttatcgc tctacaggtg attccctcac ctgaactaac gatggccatt
     1141 cgcagttacg gtgcagaggt gaaggtaatt aaacctgcat ggttggctaa agagataaag
     1201 agcggacttc aatctgcatt gaaaaactat gaatgaataa cacggaaaaa atagaccgca
     1261 ttgctaataa gcaagtcgac ggaagatata tccacggcat tagtttatgt attcagaatg
     1321 aaagtcggga tttggatcac cttatttcgc ttgggaatct aacagacgat catccgtatt
     1381 ttattgccag caccaccaaa ctttttacca ctgccatcat tttacaactc agaaacgaaa
     1441 acaaactgcg tttggaagat ccgatcggtt tatttctctc tgcagatcaa ttggcaggtt
     1501 tgcatcagtt gaaaggaatt gactcgattt caaaaattac gatcaaacat ttacttgcgc
     1561 aaacatcagg aataggggat tatttttcag gtaagcctaa gggcggggtt tcactggaga
     1621 aagaattaat ggaagggaaa gatcgttatt ggtcgtttga agaagccatg gaccgtgcgc
     1681 gcaccatttc acccgcattt tttccgggaa ccaatggaaa agcactctat tccgacacaa
     1741 atttccaact gctcggaaaa atcattgaaa acatcaccgg ccactcttat gcacaggcag
     1801 ttgatctgcg aataagcaag gtgcttggac tgaaaactac ctatgtgtat tcggatgaaa
     1861 cagacagaac accactctgc ttgtattaca aacggaatcc gcttagcatt tacaaagcca
     1921 tgagctcggt acaggtcgac ggtggaatag tgtcgaatgc cacagaaagt atggtttttc
     1981 tccgtgcttt ttttaacgga caattgttcc caaaatcgta tctggaagaa ttatatagct
     2041 ggaataaaat atttttccca ttgcaatacg gagttggaat tacgcggttt aaagtcccct
     2101 ggttcttttc tccctttaaa tccattcccg aaatcatagg ccattccggc ttatccggtg
     2161 catttgccta ttactgtcca gccaaaggct tataccttac aggaacagtc aatcaaattc
     2221 attccccatc ggtttcgtac cagatgctga ttcgcattct gaatttgttt taacattatt
     2281 ccccctctta accatttatt ttcactaata tattcaggga tttagcctga tttgtattta
     2341 ttttgcccaa taaattacgc aacattgatt tccagaatta tatccttttt tgtactggta
     2401 tttgcagcca aattttcaat cggccaaagc gatcactgct cgaaaccgct attaatgttg
     2461 cgaatgatca gcacctacca tatccagcca caggaaagca atgatgcctt ttctgaaaag
     2521 gtgatgaatc gactcatcag gcggatagat ccaatcggtt tactattcct tgaggaggaa
     2581 gttaaggatc tgcgcggatt ttcttctcgg ctggatgatg aatgggaaac ggagaattgc
     2641 ggttttattc gtttgttgca ggaaagttat tccaaagcga tcaatcgtgc agagcgaatc
     2701 attcagaatt tattggaaac acccttcaat tacaacgaaa aggagagcat tcagttttac
     2761 acctcggaaa atcagtttgg gaaagatgaa aatgaattaa aaattaaatg gaaacgcttt
     2821 ctgaaacttg aaatcctgga catccttttc atcagagatc ccgaaagtgg aaaaatccct
     2881 acggtagccg aggctttaaa aaatgaaaag gatgcaaggc aaaaagtcaa aaagaaagaa
     2941 gcaaaacgaa tagcgcggaa aaaagaatac ccgggaggaa ttgaaaatta cgtgatggaa
     3001 atctatctcg attgtatagc caccgccttc gatccgcata ctcagttttt cagtgagcgc
     3061 atcaacaatc agttcaagga atcactatca agcgaaacct tgagttttgg aattgattgg
     3121 acagaagcga aagatggcaa tccggaagta gcggaaatga atccgggcgg accggcatgg
     3181 aattcaggcg aaatcaacat tggtgatcag gttattttcg ttgaatggca agacggaaca
     3241 acaaccagca gcaatgacct tgatgttgag gatttcatac gaaaatcgga actggctact
     3301 accacaaaaa tcacgctcac atttcaaaaa tctgatggtc aggtgcgaaa ggtttcgttg
     3361 caaaaagaaa aaatgcgctc tgaggaaaac atcatttcag gatttattct ggaagaaaag
     3421 aaacgtatag ggtacattac cttacctgca ttttacacgc agtgggaaac catcaatccg
     3481 aaaggatgtg cgcaggatgt tgcaagggaa atcatcaaga tgaaacagga aaacatcgaa
     3541 ggactaatta tcgatctccg tttcaatggt ggaggatcga tgtatgaagc acttcagctt
     3601 gccggaattt ttattgatga aggagcactc ggaatacaaa aatattcaca gaccaaacca
     3661 cagattttta aggacccgaa caggggaaca gtctataccg gaccgctact cattctggta
     3721 aaccgccaca gcgcttctgc atccgaagta tttgcagcag caatgcagga ttacaacagg
     3781 gccattattg caggaacaac tacctacgga aaagccacag gtcaggcagt aattcccctc
     3841 gacacaactg cacaaacaaa gaaaaacaca gttaatgttg gtcctgctga ttttgtaaaa
     3901 atcacgatgc tggcactata ccgatgcaca gcaaaaacac accaggcgag aggcattgta
     3961 ccggatattg aaattcccga catcagcaat tattcatccg gaggagaatc ggaagaggaa
     4021 acacatctta agctcgattc tgtagataaa aaatcgtatt tcaagccctt tccgtcaaag
     4081 gactacacgg cattaagaga aaaatcacaa cagcgactgg caagcgacag tcttatgatc
     4141 tggctgaaaa aacatgagaa ggccattatt caatactaca atgaaaatca ggtgatcgat
     4201 cttgatatca acacctacta ttcaaattcg aatcttgagc ggaaaatctt tgaggactat
     4261 taccgcatta caggaattcc ctcagaagag attcgttttt acctgcatgc ctacgacaga
     4321 gatttgattt caatcgattc cttccgaaaa tcaattttcg aaaatcagat ggaaaatctg
     4381 aaaatggaca ggcaattgcg tgaagccttt tatatacttt ccgattatat tcaatcacaa
     4441 taaattaaat ccaaaaacaa tgaaagcaat ttgtacactt ctaattttca tttccggtat
     4501 gggaatgatc aacgctcaga aattcaccaa cgctggtgaa tacatgggtt ttattcagaa
     4561 acaataccag gaaatttcaa aagacacctg gagctacatt agcgctgttg cacacggaaa
     4621 aagcgcaaga aaagtggaga aacgcaggat ggaagttatt acaaccaccc aaaaagcatt
     4681 aaacaccatt aaaggactac agccctttga cggcgacaaa acattgcgcg actctgcggt
     4741 aagttatctt acgttgagtt taaatgtagt tcgggaagat tacggaaaga tcatggatct
     4801 ggaggcgatt gcagaacaaa gttttgatgc aatggaagcc tatctgctcg cacaggaaaa
     4861 agccggagaa aaaatggatc aagccggaga aatgatggaa gtcgttacaa aagcatttgc
     4921 tgaaaaacat ggcgttacac tgatcgaaag caaagatcag ctcggtaaaa aaatggaaat
     4981 tgcatctgag gttattaagt actataatca gatctacctt gttttcttta aatcttacaa
     5041 gcaggaagca tacatgctgg atgccgttca gaaagcagat gtgaacggag ttgaacagaa
     5101 taaaacatca ctgaatcaat ttgcaaaaga agggctggaa aaattaaaag aattcaagcc
     5161 ttataacaac gacggatcca ttattgcagc ctgcaatgag cttctaaagt tttatgttat
     5221 ggaaacagaa gaaaaaattt cttcaattac agatttcctc atcaagaaag aaaactttga
     5281 taaaaagaat gctgcatttc aggccaaaaa agaaaaagac cgtacacaag cggatatcga
     5341 tcagatcaat gcagccggaa aagaatacaa cgaagccgta aataattaca acaaaaccaa
     5401 taccgagctc ttcaataagc gtaaagcctt actcgataat tggaataaaa cggtacagac
     5461 a
//
LOCUS       DAOMYG010000203         5454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5161_length_5454_cov_10.488424, whole genome shotgun sequence.
ACCESSION   DAOMYG010000203 DAOMYG010000000
VERSION     DAOMYG010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5161_length_5454_cov_10.488424"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="PK610_07670"
     CDS             <1..1069
                     /locus_tag="PK610_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HRJ35851.1"
                     /translation="RDGRVSHAQLIAGPEGSGTLPLALAYAQYLLCSNKQENDSCGTC
                     SSCIRVSKLEHPDLHLSFPIVKEGTKVEKSDDRIAEFRKVFLEQPYMPLRHFLSELEE
                     ESKRPLIGTEEAGAIIHKLNYKSYEGEYKILVMWMAEFMNEVAANNLLKIIEEPPDNT
                     VFLLIAESTESILPTILSRTQLIKVPALTDDEMMWVLTEGHSIPEEEAYNITMLAEGN
                     YWAGLSMVLDLEDSGFSMQTFRAWMLLCHKKDIAGLISWSDEMASLTREQHKNFVRYA
                     LHIFRQCIIGAYTNNELLRAREDERDFISKFSQFVHGYNIVALMEEFNKSHYYLERNA
                     NPKLVFINLSFKVTALMFPKK"
     gene            1175..2602
                     /gene="ricT"
                     /locus_tag="PK610_07675"
     CDS             1175..2602
                     /gene="ricT"
                     /locus_tag="PK610_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019976822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRJ35852.1"
                     /translation="MGCESCSSKDGSPSGCRNNGLCSTGGCNKLEVFDWLANMELPAG
                     HKPFDLVEIRFKNGRKHFFKNTQHLPLHTGDAVAVEASPGYDVGVVTLTGELVKMQMQ
                     RRNVSPESSELRKIMRKANEADIEKWKEARSLEYETMHKARKMAIDLGLEMKISDVEY
                     QGDRTKATFYYTAEGRVDFRELIKVMAEQFKVRIEMRQIGARQEASRLGGIGSCGREL
                     CCSTWLTDFRTVSTSAARYQQLSLNPQKLAGQCGKLKCCLNYELDMYLEAYKEFPDNS
                     IRLQLKNGYAAHVKTDIFKRMMWYAAPGEFGITNIVGLKIDRVKEIIALNKNGQQVDD
                     LKGFVEVVEVVKEPDFDNVVGQDSLTRFDEKRNKKKGRHKDRRKDGKGGVQQQNTQGQ
                     NQGKPQGQNRPQQQGQQHQRSQHPHNRPQQKQQGEQGQQNQQGGQPKQNQQGQGGQGQ
                     RRDNKNRNRGPRRDNRPPKNDQPTT"
     gene            2602..3072
                     /locus_tag="PK610_07680"
     CDS             2602..3072
                     /locus_tag="PK610_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HRJ35853.1"
                     /translation="MMRIRFFVPLLFVLTLASCGDKPFFQQNTAIEDQSWNWNEPVSY
                     EVQVDDTVSSYNFYLDLRVTKSYEYSNIYVFFTTVFPDGKTARDTIQANLQDEFGNWY
                     GKPTGSLVDNRILFGPKRVFPVAGKYGFTIEQAMREKDLTEVADVGLRIERSKK"
     gene            3072..>5454
                     /locus_tag="PK610_07685"
     CDS             3072..>5454
                     /locus_tag="PK610_07685"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRJ35854.1"
                     /translation="MAKENSEKKSVDSGLGKRGKRMVLAIWWMIALGPIVSVWLVMFF
                     VSMGNLPSFDELENPSSNEATMIISGDGQILGKYFKENRTNVKYNQISRFVIDCLVAT
                     EDERYYDHSGIDFKALPRVFSGAITGNTNRGGGSTITQQLAKMLFHDKAKSTLERVKQ
                     KLQEWVIAVRLERAYTKEEIITMYLNKFDFVNNAVGIHSAARVYFNTTPDSLKIHEAA
                     LLVGMCQNPAMYNPKRFPERAMKRREIVLYQLMRSSENPLVKTKITREQYDSLRVLPI
                     ELDFQSTDHVEGPAPYFREELRKELTALFEKKDENGNFVYHKKDGSPYNIYNDGLRIY
                     TTLDSRMQRYAEWAVAEHLGKELQIDFDKDNKRWKNPPFSNDITKEKGDSLILASVHR
                     SKRYRILSGKLCGNCERTKDMAKKGDFHICGFCGDSLAYRTENEIDKIFNTPTKMRVF
                     TWRKPGNEFDKLMSPRDSIIYYKRFLQVGMVSMDPHTGFIKAWVGGPNFKHFQFDHVQ
                     VGKRQVGSTFKPFVYAAAFRDRVLSPCSTAPDIEHCIEVPLNARTNKLWCPRNAGVKY
                     TGEQIPLFWALAASMNNITAFIMQKEKPALVIKLVEDLGIPKGYLDPVPAICLGVCDL
                     SVLQIVAAQSAFANKGVYIKPMMYTRIEDRQGNVILDVQPETNEAMDEHTAYAMLEIM
                     KGATSGAINPFTGKVAGTALRMRSGKPYGGIKTAVAGKTGTTQNNSDGWFIGLTPDLV
                     TGVWVGCEDRSVRFSRTDLGQGANTAMPVWGYYMKKVYADKQLKISTSDFERPD"
BASE COUNT         1640 a         1095 c         1307 g         1412 t
ORIGIN      
        1 ccgcgatggc agggttagtc atgctcagct gattgcaggt cctgaaggct caggtacact
       61 tcctttggcg ctggcttatg cgcaatattt actttgttcc aataagcagg aaaacgactc
      121 ctgtggtaca tgctcgtctt gtatccgtgt ttcaaaactt gaacatcccg atcttcatct
      181 ttcttttccc attgtaaaag aaggcacgaa agtggagaaa tccgatgatc gtattgcaga
      241 gtttcgaaag gtttttctcg aacagcccta tatgccgctt cggcattttt tatcggaact
      301 cgaagaagaa agtaaacgtc cgctgatcgg gacagaagaa gcgggagcca ttattcataa
      361 actgaactat aaatcctatg agggggaata caaaattctt gtcatgtgga tggctgagtt
      421 catgaatgaa gtggctgcaa ataatttgct gaaaataatc gaagaacctc cggataacac
      481 agttttcctt cttattgcgg aaagcacgga aagcattctt cctacaattt tgtcgcgtac
      541 gcaactcatc aaagtccctg cgttaaccga cgatgaaatg atgtgggtat taaccgaggg
      601 gcactctatt ccggaagagg aagcgtacaa tattaccatg cttgccgaag ggaattattg
      661 ggcagggctt tccatggttc ttgatcttga agattcaggg ttcagcatgc aaacattccg
      721 tgcatggatg cttttgtgcc acaaaaagga tatcgcggga ttgatttcct ggagtgatga
      781 aatggcttcg ctcacaaggg aacagcacaa aaactttgtg cgctatgcct tgcatatttt
      841 caggcaatgt atcattggtg catacaccaa taatgagcta ttgcgcgcaa gggaagatga
      901 acgggatttt atttcgaaat tctctcagtt tgtgcacgga tacaacatcg ttgcgcttat
      961 ggaagaattt aacaaatcgc attattatct cgagagaaac gccaatccca aactggtttt
     1021 catcaacctt tcgtttaagg ttactgccct gatgttccca aagaaataag cctttttttg
     1081 cgcataatag gcgattgcct tcgtctgcta tttttcgaat ggaatatgcc gctttttcgt
     1141 acatttggac tttcaacaaa aaaccaccaa aacaatggga tgtgagagtt gctcaagcaa
     1201 ggatggaagt ccttccggat gccggaacaa cggtctttgc agtactggtg ggtgtaacaa
     1261 gctggaagtt ttcgactggt tggccaatat ggaattgcct gcagggcata agccattcga
     1321 ccttgtagaa atccggttta agaatggtcg caagcatttt tttaagaata cacaacacct
     1381 tccgctccat accggtgatg cagttgccgt tgaggcaagc cccggttacg atgtgggagt
     1441 tgtcaccctt accggtgaat tggtaaaaat gcaaatgcag cgccgaaacg tttcacccga
     1501 gtccagcgaa ctgcgtaaga tcatgcgcaa agccaatgag gctgatatcg aaaaatggaa
     1561 agaagctcgt tcgctcgaat acgaaaccat gcacaaggcc cgcaaaatgg caattgatct
     1621 tggtctcgaa atgaaaatca gcgatgttga gtatcagggc gatcgaacaa aagctacgtt
     1681 ttactatacc gcagaaggtc gcgtcgattt tcgtgagctg atcaaggtga tggctgaaca
     1741 attcaaagtg cgtatcgaaa tgcgccagat cggcgcacgt caggaagcgt cacgcctcgg
     1801 tggaataggc tcatgcggtc gtgaattgtg ctgctcaacc tggcttaccg atttcagaac
     1861 agtgtccacc agcgctgccc gttaccaaca actttcactg aatccgcaaa aacttgccgg
     1921 acaatgcggg aagctgaagt gctgcctcaa ttacgagttg gatatgtacc tggaagctta
     1981 caaggagttt ccggataaca gtatccgcct tcagctgaaa aacgggtatg ccgcccacgt
     2041 aaaaacagat atttttaaac gcatgatgtg gtacgccgcc ccgggtgagt ttggtattac
     2101 caatatcgta ggattaaaaa tagatcgcgt caaagaaatt attgccctga ataaaaatgg
     2161 tcagcaggtt gatgatctga aaggctttgt tgaagtggtt gaagttgtaa aagaacccga
     2221 tttcgacaat gttgtgggtc aggattcact tacccgattt gatgaaaaac ggaataagaa
     2281 aaaaggacgt cacaaagacc gccgtaaaga cggaaaaggt ggcgtgcaac aacaaaatac
     2341 acaaggtcag aaccaaggga aacctcaagg tcaaaaccgt ccgcagcaac aagggcaaca
     2401 acaccagcgt tcgcaacacc cgcataatcg tccgcaacaa aaacaacagg gagagcaggg
     2461 gcagcaaaat cagcaaggag gacagcccaa gcaaaatcag caaggacaag gtggtcaggg
     2521 tcagcgtcgc gataacaaaa accggaatcg cggaccgcgt cgcgataatc gtccccctaa
     2581 aaatgatcag cccactacct gatgatgaga attcgctttt ttgttccgct tctttttgtg
     2641 ctgacacttg catcgtgtgg cgacaaacct tttttccagc agaatacagc tattgaagac
     2701 caaagctgga actggaatga accggtgagt tatgaagtgc aggtcgatga tacggtaagc
     2761 agctataatt tctacctcga tttgcgcgtg acaaagagtt acgaatacag taatatctat
     2821 gtgtttttta caacggtgtt cccggatgga aaaactgcac gcgataccat tcaggctaat
     2881 ttgcaggatg aatttggaaa ctggtatgga aaaccaaccg gatcattagt cgataaccgg
     2941 attttattcg gacctaaacg cgtatttcct gttgcaggga aatatggttt tacaattgag
     3001 caggcaatgc gggaaaaaga ccttaccgaa gttgctgatg taggattacg gattgaacga
     3061 agcaagaagt aatggcaaaa gaaaattcag aaaagaaatc agttgacagc ggtttaggga
     3121 aacgcggtaa acgaatggtt ctggccatat ggtggatgat tgctttgggg ccaatcgtgt
     3181 cggtgtggtt ggttatgttt tttgtttcaa tgggaaatct tccatcattc gatgaattgg
     3241 aaaacccaag cagcaacgaa gcaaccatga tcatttccgg cgatggtcaa attcttggga
     3301 aatatttcaa agaaaaccgt accaatgtaa aatacaatca gatttcccgc tttgttatcg
     3361 attgtctggt tgctacagaa gatgaaaggt attacgatca ttcaggaata gattttaaag
     3421 cactgccacg cgtgttttca ggtgcaatta ccgggaacac caacagaggg ggaggaagta
     3481 cgattaccca gcagttggca aaaatgcttt ttcacgataa agcaaaaagc acgctcgaaa
     3541 gggtaaaaca gaaattacag gagtgggtaa ttgctgtgcg attggaacgg gcgtatacta
     3601 aggaagagat cattacgatg tatctcaata aatttgattt tgtaaacaac gcggtaggaa
     3661 ttcattctgc agccagagta tatttcaata ccactcctga ttcgctgaaa attcatgaag
     3721 cggccttgtt ggtgggaatg tgccaaaacc cggcaatgta taatcccaaa cgttttcctg
     3781 agcgtgcaat gaagcgtcgg gaaattgtgt tgtatcagct tatgcgtagt tccgaaaatc
     3841 cattggtaaa aacaaaaatt acccgtgaac agtacgactc acttcgggta ttgcctatag
     3901 aacttgattt tcaatcgacc gatcacgtag aaggtcctgc accttatttc cgtgaagaat
     3961 tgcgtaagga gctgactgcg ctttttgaga aaaaagatga aaacgggaat tttgtttacc
     4021 ataagaaaga tggttctccc tataatattt acaacgacgg attacgaatc tacactacac
     4081 tcgattcgcg catgcaacgg tatgctgaat gggctgttgc tgaacatctg ggtaaggagt
     4141 tgcagatcga ttttgacaag gacaataagc gctggaagaa tccacctttt tccaatgata
     4201 ttacaaaaga aaaaggagac tccctgatat tggcatctgt tcatcgctct aaacgataca
     4261 gaattttgtc cggtaagttg tgcggaaatt gcgagcgtac taaagacatg gccaaaaaag
     4321 gcgattttca tatttgtgga ttttgcggtg attcacttgc gtatcggaca gaaaacgaga
     4381 tcgacaaaat ttttaataca ccaacgaaaa tgcgggtgtt tacctggaga aaaccaggaa
     4441 atgaattcga taaactgatg agtccgcgcg attcaattat ctattacaaa cggtttttac
     4501 aagtgggaat ggtgtcgatg gatccgcata ccggatttat taaagcatgg gttggcggac
     4561 caaatttcaa acatttccaa ttcgatcacg tgcaggttgg taaacgacag gttggttcaa
     4621 cattcaagcc atttgtttat gcagcagcat tccgcgatcg tgtgttaagt ccatgttcta
     4681 ctgctcctga tattgaacat tgtatcgaag ttccattgaa tgcaagaaca aacaaattgt
     4741 ggtgtccgcg taatgcagga gtaaaatata ccggcgagca aatcccctta ttctgggcgc
     4801 ttgcagcatc aatgaataac atcacggcgt ttatcatgca aaaggaaaaa cctgctttgg
     4861 taatcaaact ggttgaggat cttggaatac caaaagggta tctcgatcct gttcctgcaa
     4921 tttgtttggg cgtatgcgat ctttccgtat tacaaattgt tgctgcgcag agtgcatttg
     4981 caaataaggg agtttacatc aaacccatga tgtacacccg aattgaagat cgtcagggaa
     5041 atgttattct cgatgttcaa cccgaaacca atgaggcgat ggatgagcat acggcttatg
     5101 caatgctcga gatcatgaaa ggagcaacaa gtggtgcaat aaatccgttt acaggaaagg
     5161 ttgccggaac agctttgcgt atgcgtagcg gaaaacctta tggcggaatt aaaacagcag
     5221 tagcaggtaa aacgggaaca actcagaaca attccgatgg ttggtttatt ggtttgacac
     5281 ctgatcttgt taccggagtt tgggtgggtt gcgaagatcg ttcagttcgt ttttccagaa
     5341 ccgatcttgg tcagggcgcg aatactgcaa tgccggtttg gggttattat atgaaaaagg
     5401 tgtatgcaga taagcagtta aaaatttcta catccgattt cgaacgacct gatg
//
LOCUS       DAOMYG010000204         5423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5191_length_5423_cov_14.114382, whole genome shotgun sequence.
ACCESSION   DAOMYG010000204 DAOMYG010000000
VERSION     DAOMYG010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5191_length_5423_cov_14.114382"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PK610_07690"
     CDS             <1..941
                     /locus_tag="PK610_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ35855.1"
                     /translation="LLALYFTSCVPQRKYEELEAKYKSCDQELTKLRDSSRQAIDAYK
                     ELSSQHERLKKATDALGRDTSILGASYRQMKEQYDKINKLNDEIMRKIEFLQKGSEAE
                     ASKLSVQLEATRLELQRKEDALKLFERELNVKKSELEAKEKRIKELEDILAKQEAASK
                     ALRDKVANALKAFEGKGLTVVQKNGRVYVSMEAKLLFASGSYKVDQEGKDAIVKLSKV
                     LEDTKDIEILVEGHTDTDKLNSPNVPRDNWELSVLRATSVVKMMLESSRMDPKRISAA
                     GRSEFLPVDAADKAKNRRIEIVLIPNLDELYKALDN"
     gene            complement(1009..3150)
                     /locus_tag="PK610_07695"
     CDS             complement(1009..3150)
                     /locus_tag="PK610_07695"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRJ35856.1"
                     /translation="MKRTLIVWSLLLVSLTLRADEGMWLLMHLKKMNMEDMQKKGLRL
                     TAEEIYDINKPGLKDAVVSLGGFCTAEVISKEGLLLTNHHCAFDAIQTFSTVENDYLT
                     NGFWAMDRKQELNVPGLFVNFLVRMEDVTEKVLAAGANMGVTARNEAMRRKMEELRKE
                     ATKGTHYTAEIKSFFDGNEYYLFIYETYRDVRLVGAPPSSIGKFGGDTDNWMWPRHTG
                     DFSLLRVYSGADGKPAEYSDKNIPLKPRHHLPVSLKGVNQSDFAMIMGYPGSTDRFLT
                     SYGVKQAIETEQPARVRIRGTRLEIMKADMDQSDKVRIQYASHYAAIANYWKYFIGQS
                     RGLKRLRVYEQKKAEEVAFQEWVNADAKRKIRYGQVLSDMEAAYSELDKVIVPDVYFN
                     ECIFGIEINTLFLKLYPLYGALNNTDITKDQLNGVVESVRTAVKDYFKDHNAPTDAKL
                     MAAMLRLYHSDVPAEFHPKELTDIHKKTKGDFMKWTVPYYAKSIFTDATRLDAFLKAP
                     EMKKMKADPGFALMLAFIDVYRTFSERSSAASSKLAEATTLYVEGIRILRTDKKFYPN
                     ANFTMRLTYGQVLEYFPQDGVHYLHYTTLDGIMEKEDPNNDEFVVPARLKELWKNKDY
                     GQYGQNGVLNVAFITNNDITGGNSGSPVINGNGELIGCAFDGNWEAMSGDIAFEHKLQ
                     RTISVDIRYVLFIIDKYAGAKHLVDEMTLVK"
     gene            complement(3285..>5423)
                     /locus_tag="PK610_07700"
     CDS             complement(3285..>5423)
                     /locus_tag="PK610_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35857.1"
                     /translation="NYTPCNAPVVTITQPAAAVTNASASISGTVQHHAGTTISVSVNG
                     TPVNATYSNGNFTANATLASGSNTILVTASGSCGTASSTATVTYTAPAPAPCGPRFNP
                     GNSAWEFCLITPSGTYTRDNLAANPNFTYTGPASSAYFKPIAGGGDAIVNGSAYPVQN
                     GSYYHFSGNLTVTVGSNQPGSMGHWTICIESNAAPVFGNGNNRPASPCQSTNQGGGNN
                     SGNGDSTVDFDINPNGSVTVSQTVCANIKCLGESVVYSNTQDAFVKISYSIDNGVTWS
                     NFNNNNYVNGGEQESVLVPTGSNVVLKAFCTTANGSWTNTIKTNTGSQYVYVLRNGDP
                     APNIQPFQNQASAEAFLAGVMTNGVITIGPNDVIYLFELRFVGNVGIDYQDAVMLVTF
                     DENGNCSNDNRGNTGGGSTGGNNSGRGNNKSPENTGGNTGTQNKGDGNINPNNNVNPN
                     NTGGKTNPVEGGSTNGVSPNNNTGGKTDPVNSGKDVQNNTPVNNREYTEAISAGDRSF
                     AEQRYADAKTHYARALTLKPGDPYATQKIKDCDQMLDRKRIEEDAQRKAQEDAQKKAQ
                     QDAQRKAQEDAQRKAQEDAQRKTQEDAQRKAQEDAQRKAQEDAQRKAQQDAQRKAQED
                     AQRKAQEDAQRKTQEDAQRKAQEDATKKAAAEKAAADAKKKQEEEAKKKAEEEKKKQE
                     SEKKAADPKTLPGGKVGVTVNEEGVKQNPK"
BASE COUNT         1355 a         1215 c         1230 g         1623 t
ORIGIN      
        1 ctttgctggc actttatttt acatcctgtg tgccacagag aaagtatgaa gaacttgaag
       61 ccaagtacaa atcatgtgat caggaactca cgaaactgcg ggatagctca cgacaggcaa
      121 ttgatgccta taaagaactt tcttcgcaac atgagcgtct gaaaaaggct acagacgctt
      181 taggtcgcga tacctctatt cttggcgctt cataccgtca aatgaaggag caatacgaca
      241 agatcaacaa gttaaatgat gagatcatgc gcaagatcga gttcctgcaa aaaggaagcg
      301 aagctgaggc ctccaaatta tctgttcaac ttgaagcgac acgactggaa ttgcagcgca
      361 aggaggatgc cttgaaattg tttgagcgcg aactgaatgt gaaaaaatcc gaattggaag
      421 ccaaggaaaa acgcatcaaa gaacttgagg atattctcgc aaaacaagaa gcagcttcaa
      481 aagcattacg cgataaagtg gctaatgcac ttaaggcttt tgaaggaaaa ggcttgacag
      541 tggttcagaa gaatggtcgc gtttatgtga gtatggaagc gaaactcttg tttgcatcgg
      601 gtagctacaa agtagatcag gaaggaaaag atgccattgt gaaactttca aaagtgcttg
      661 aagacaccaa agacattgaa attctggttg aaggacacac cgacactgat aaactgaact
      721 cgccaaatgt accgcgtgat aattgggagc tttctgtatt gcgcgccacc tcagttgtaa
      781 aaatgatgtt ggagagcagt cgaatggatc caaaaagaat ctctgcagcc ggtagaagcg
      841 agtttcttcc tgttgacgca gcggataagg ctaagaatag aagaattgag attgttctga
      901 ttccaaatct tgatgagtta tacaaagctt tggataatta atcctattca aataaaaaga
      961 aaagcccctg aattttcagg ggcttttctt tttgaatgta ttagataatt atttcaccag
     1021 tgtcatttca tcaacgagat gtttggctcc ggcatactta tcaataatga aaagaacata
     1081 gcggatatca actgaaatgg tgcgttgcag tttatgttca aacgcaatat cgccgctcat
     1141 ggcttcccag ttaccatcaa aggcacaacc aatcaactct ccgtttccgt taatcaccgg
     1201 actaccggag tttcctccag tgatatcgtt gttggtaatg aatgcaacat tgagaacccc
     1261 attctgtccg tactgaccat aatctttgtt tttccaaagt tctttcaaac gtgcaggcac
     1321 aacaaattca tcattgtttg gatcttcctt ctccatgatg ccatccaggg tcgtgtagtg
     1381 gagataatga accccatcct gagggaaata ttcaagcacc tgtccgtagg taaggcgcat
     1441 ggtaaagttg gcgttggggt agaatttttt atccgtacgc aggatgcgaa ttccttctac
     1501 atacaaagta gtcgcttctg cgagtttact gctggctgca cttgaacgct ccgagaaggt
     1561 gcggtacaca tcaatgaaag ccagcataag tgcaaacccg ggatctgctt tcatcttctt
     1621 catctcgggc gctttcagga aggcatcaag acgtgtagca tcagtaaaga ttgatttcgc
     1681 ataatagggt acggtccact tcatgaaatc accctttgtc ttcttatgga tatctgttag
     1741 ttcttttgga tgaaactcag caggaacgtc tgaatgataa agacgcaaca tagcggccat
     1801 taatttggca tctgttggtg cattgtgatc tttaaagtaa tcctttacag cagtacgaac
     1861 actttcaaca acgccattca gttgatcctt ggtaatgtcg gtattgttca aggcaccata
     1921 caatggatac aacttcagga acaaggtgtt gatctcgata ccaaagatgc attcgttgaa
     1981 atagacatcg ggaacaatta ctttatccaa ttcagaatag gccgcttcca tatcgctgag
     2041 cacctggccg tagcggattt tacgttttgc atccgcattt acccattcct gaaaggcaac
     2101 ttcttccgct tttttctgct cataaacacg aagtctttta agtccgcggc tttgtccgat
     2161 aaagtatttc cagtaattgg caatggcagc ataatgggag gcatactgaa tgcgtacttt
     2221 gtcgctctga tccatgtctg ccttcatgat ctccaaacgg gttccgcgaa ttcggacacg
     2281 ggcaggttgt tcagtttcaa tggcttgttt taccccgtaa gaagtgagga aacgatcggt
     2341 gcttccggga taacccatga tcattgcaaa atcgctttgg ttcacgcctt tcaatgaaac
     2401 tggcaggtgg tgacgaggtt tgagtggtat gtttttatcc gaatattcag ccggtttgcc
     2461 atctgcgccg gaataaacgc gcaatagaga gaaatcaccg gtatgacgtg gccacatcca
     2521 gttgtccgta tcacctccaa atttcccgat ggaacttggc ggggccccaa ccaaacgcac
     2581 atcgcggtag gtttcataga taaaaagata atattcgttt ccgtcgaaga atgattttat
     2641 ttctgcagtg taatgggttc cctttgtagc ttctttacgg agctcttcca ttttacggcg
     2701 catcgcttca ttgcgggctg taacacccat gttggcgcca gccgcaagta ccttttctgt
     2761 aacatcttcc atgcgaacca gaaaattcac aaacaaaccg ggcacattca attcctgctt
     2821 acgatccata gcccaaaagc cgttggtcag gtaatcgttc tctacggtgg aaaaggtttg
     2881 aattgcatca aacgcacaat ggtgattggt aagcaacaag ccttctttgg aaataacttc
     2941 tgcagtgcaa aatcccccca gagagacaac agcgtctttt agaccgggtt tgttgatgtc
     3001 atagatttct tctgcagtga ggcggaggcc ttttttctgc atgtcttcca tgttcatttt
     3061 cttcaggtgc atcagcagcc acatcccttc gtcggctctc aaggtgaggc ttacgagtaa
     3121 cagggaccat acgatcagtg ttcttttcat ttcttaaaaa ttggaaagcg aagataaaaa
     3181 gtatcggttc tgttgcgtgg ctttttcgat gagtggtaaa aatggaccac caagcgcaga
     3241 tttagggcat aaaaaaggcg ggcctaggcc cgccttatac tatgttactt ggggttttgc
     3301 tttacgccct cttcgttaac cgttacccca acctttcctc cgggaagggt tttagggtca
     3361 gctgcttttt tttcagactc ttgtttcttc ttttcttctt cggctttttt cttggcttct
     3421 tcttcctgct ttttctttgc atcagccgcc gctttttcag ccgctgcttt cttggtagca
     3481 tcttcctgag ccttacgctg tgcatcttcc tgtgtctttc gctgagcgtc ttcctgtgct
     3541 ttacgttgag catcttcctg agccttgcgc tgcgcatctt gttgtgcctt tcgctgagcg
     3601 tcttcctgtg ctttacgttg cgcatcttcc tgagccttac gttgagcatc ttcctgtgtc
     3661 tttcgctgag cgtcctcctg tgctttacgt tgagcatctt cctgggcctt gcgctgcgca
     3721 tcttgttgtg cttttttctg agcgtcttcc tgtgccttac gctgggcgtc ctcttctatg
     3781 cgcttacggt cgagcatttg atcgcaatct ttaatttttt gagttgcata aggatcgccc
     3841 ggcttaagtg tgagtgcacg tgcataatgc gtttttgcat cggcataccg ctgttcagca
     3901 aacgaacggt cgccggcact gatggcttct gtgtactctc tgttatttac gggagtgttg
     3961 ttttgaacat ctttcccgga attaactgga tcagtttttc cgcctgtgtt attgttgggc
     4021 gatacaccgt tggtacttcc tccttcaaca ggatttgttt tccctcctgt attgttgggg
     4081 tttacattgt tgttggggtt aatattgcca tcacctttgt tttgagttcc ggtgtttcct
     4141 ccggtgttct ctggagattt gttattgcct cttccactgt tgtttcctcc ggtggatcct
     4201 cctccggtat tacccctgtt atcgttcgag caattcccgt tttcatcaaa cgttacaagc
     4261 ataacagcgt cttgatagtc gatgccgaca ttcccgacaa accgcaattc gaagaggtag
     4321 attacatcgt tcggaccaat cgtaataacg ccatttgtca taactcccgc cagaaacgct
     4381 tctgctgacg cctgattttg gaagggctga atgtttggag cgggatcacc gtttctcaat
     4441 acatacacat actgacttcc ggtgttggtt tttattgtat tggtccagct tccgtttgca
     4501 gttgtacaaa acgctttcaa cactacgttg gatccggtgg ggacaagaac ggattcttgt
     4561 tccccgccat ttacataatt gttgttgttg aagtttgacc aggtaacacc attgtcgata
     4621 ctataggaga tttttacaaa agcatcctgt gtgttgctat aaacaactga ttctcccagg
     4681 catttgatgt ttgcgcaaac cgtctgacta acggtgacag aaccattagg attaatatca
     4741 aaatctacag tagaatcacc attaccggag ttgtttcctc caccctggtt ggtgctttgg
     4801 caggggctag ccggacgatt attaccatta ccaaaaacag gagcagcatt cgattcaata
     4861 cagatggtcc agtgacccat cgatccgggt tggttgctgc caactgttac ggttaggttt
     4921 ccgctaaagt ggtagtacga accattttgt acaggatacg ctgatccgtt aacaattgca
     4981 tcaccaccac ctgcaatggg tttgaaataa gcactgcttg caggaccggt ataggtgaag
     5041 tttggatttg cagcaaggtt atctctagta taagttccgc ttggagtaat caaacagaat
     5101 tcccatgctg aatttccggg attgaaacgt ggtccgcaag gagcaggagc aggcgctgta
     5161 taagttaccg ttgcagtact gcttgccgtt ccgcaagaac cggatgctgt aactaaaatg
     5221 gtattgcttc cgcttgctaa tgttgcgtta gccgtgaaat taccattgct ataagttgca
     5281 ttaacaggtg ttccgtttac actcacagaa atcgttgttc cggcgtggtg ttgaacagtt
     5341 ccgcttatgc ttgcagatgc gttggtaacc gctgcagcag gttgtgtaat agttacaaca
     5401 ggggcattac atggagtgta att
//
LOCUS       DAOMYG010000205         5394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5232_length_5394_cov_7.752575, whole genome shotgun sequence.
ACCESSION   DAOMYG010000205 DAOMYG010000000
VERSION     DAOMYG010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5232_length_5394_cov_7.752575"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1783)
                     /locus_tag="PK610_07705"
     CDS             complement(23..1783)
                     /locus_tag="PK610_07705"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35858.1"
                     /translation="MIVKYSLSFNQPQHRFIDVVVRIPAEKRKSVEVQLPAWRPGRYE
                     LGNFAKNVRRFAVTNEQGHALPFEKLNKDSWKIQVSGSEEIVVSYQYFSGDLNAGSTF
                     VDATQLYVNPVNCLIYDKERMNEGCEVLINVPAGWQLACGCKWHQESTEQGKNAFRLF
                     PMDFHELADSPFISSAELRHNDFIVEGTTFNIWIQGECRPDWERIIEHFSAFAKSQIE
                     AYGEFPVQSYHFLLQVLPVNFYHGVEHLTSSVNALGPGYELMRERYDDLLGLCSHELY
                     HAWNIKSIRPVEMFPYDYSKENYFKTGYVAEGVTTYMGDYFLHASGVFNLAAYFSELS
                     AQLQKHMDNFGRRNYSVADSSFDTWLDGYVPGVAERKVSIYTEGCLLAFIADVEIMRH
                     SKNKHRLHDVMYDLYHDFSKKGLGYSAKDYRKLIEKYAGNGMCSYFDTLINGAGDYLP
                     MLNNALEYLGWAVLSYDPPSVAERLYGMKISDSSAGSFLMQFHPDEPVALSGMAQGDK
                     LISVNGYLVNNDANKWIAYFGGENMQIAVLRGSRMLDFSISPAINGGFRTWRIIKADS
                     QTPAQEENYRNWATVKSKIK"
     gene            complement(1792..3195)
                     /locus_tag="PK610_07710"
     CDS             complement(1792..3195)
                     /locus_tag="PK610_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35859.1"
                     /translation="MLLTDKEIIEFGGVLKNCARNTLLQLWKAEGLNRKTEASEVYRK
                     VFGEPYSSKKDARLRHERRLLRDKLKEFLSARYLKRSGEIHEHNRDILYLEELLSRKE
                     WSTFLNEVKSVRKKLEIRHDQDGLIRVIRMELSVYVNQGKTEKENIQTIAELNKQLLS
                     LEHLVFDRKKAQAESVHVWCNRWMGESDTESLAQKINASISNEEEDKVVRYFRLKTES
                     LRAVSDRRMEQLEEMRSLAESIDDFQFPWSRELAVILSNLALEYSFRQQFDHSVELFR
                     ESLKYRKSIPEVNVHQIVYNLIGVLLRCEKIKDALELIETEGHALQRDPFLFSRFGLL
                     KGMALLLNGNWQDARVLHQELSGKLSEHDDLYHRVQELIILVMRNKRDTAERHIRNLR
                     DLLRGRTDVEHYKVFLKVLASCFKLENESISVKNALLRTIDPAVYSRGNVLPLIWLRK
                     WIEQNTKPSAGTGRGNA"
     gene            3292..4143
                     /locus_tag="PK610_07715"
     CDS             3292..4143
                     /locus_tag="PK610_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35860.1"
                     /translation="MFRILLAATFLIFTSVEGTAQVRQLMLEKAKQMSTTKQQSTQPA
                     DTTQKATPSNEPVKQEQQSNPIGFSGGSKKDVRPVYTFNDNMLVEITNYKKDGKKDGD
                     PVKVRYLFSKEAYLGSEMTIEGKKPEDKTESTSIVEFGKNQMVMLSKTKDGNSGFVMK
                     YDSQKIQEKAAKDTSSAPKFTKTGRTKTILGYLSEEWVSTDEKGNKTEIWISNQVKLD
                     VSGASAAFAGAKGSKSVNTQGYPQGAMLEMNYYEKNGEKTTWIVLEINLNKTTTISTE
                     GYTFMGF"
     gene            4156..5112
                     /locus_tag="PK610_07720"
     CDS             4156..5112
                     /locus_tag="PK610_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35861.1"
                     /translation="MRTTLLTVVVMTTLNSVAYSQTTANDGDWKKNYVVLNNTPQAEH
                     MIRGGDIDNLGFGWENNFNPFSGKSTETHFYPWTPDTTFAGTDRILIGSSVGKSEPPC
                     SADGYSGEWNALKTQFGKTTQAIKIPLSIPATTIVKDVMLQLFVDDFQSPVFCSSFQV
                     LLNGKRAPFIENIINKLNQSGPIGKLITIEIPAEYLSLFKEKQLEILIDDPTTGAHDG
                     YAIDFVKVLINPKPGAFKKYNHTVLVKDNLTGDPLKGVIVELANGKIVRTNDKGEAIL
                     QDLVLGYHPIRLTHCGYHEVFTGVDIEEKPEAPQTFVMEVQQ"
     gene            5249..>5394
                     /locus_tag="PK610_07725"
     CDS             5249..>5394
                     /locus_tag="PK610_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ35862.1"
                     /translation="MMSYDLIVLGSGPGGYVAAIRASQLGLKTAIVERESLGGICLNW
                     GCIPT"
BASE COUNT         1656 a         1249 c         1018 g         1471 t
ORIGIN      
        1 aggttttcca tctgcacttc tctcatttta tttttgattt tacggttgcc cagttacggt
       61 aattttcttc ctgcgcaggt gtttgcgaat cggctttaat tattcgccag gttctgaaac
      121 cgccgttgat agcaggagag atggaaaaat ccaacatgcg gctacctctt aaaacagcga
      181 tctgcatatt ctctccgcca aaataggcaa tccatttatt tgcgtcgtta ttaaccaggt
      241 atccgtttac cgaaatcaat ttatcaccct gtgccattcc tgaaagagca accggttcgt
      301 cgggatggaa ctgcatcagg aaacttcctg cagatgaatc agaaattttc attccgtaca
      361 atcgctcagc gacacttgga ggatcataac tcaacactgc ccaacccagg tattcgagtg
      421 cattgttcag cattggcaaa taatctccgg caccattaat caaagtatcg aaataactgc
      481 acataccgtt tccggcatat ttctctatga gttttctgta atcctttgca gaataaccta
      541 atcctttctt actgaaatcg tggtacagat cgtacatgac atcatggagg cggtgtttgt
      601 tcttgctgtg acgcatgatc tcaacatccg caataaatgc caataaacaa ccttctgtat
      661 aaattgaaac ttttcgctcc gcaacaccgg gcacataacc atcaagccag gtatcaaatg
      721 aagaatctgc aactgaataa tttcttctac cgaaattatc catgtgcttt tgtaactgtg
      781 cgctcaactc agaaaaatag gcagcaaggt taaatacccc ggatgcatga agaaaataat
      841 ctcccatgta cgtggttaca ccttctgcaa catatccggt tttgaaataa ttttctttgg
      901 aataatcata aggaaacatt tctacaggac gaatactctt gatattccag gcgtggtata
      961 attcgtgcga acacagccct aacagatcgt cgtaacgctc ccgcatcaat tcataacctg
     1021 gtcccaaagc attaacacta ctggttaaat gctctacacc atggtaaaaa tttaccggaa
     1081 gcacctgcaa caggaaatgg taggattgaa caggaaattc accataagct tcaatttgtg
     1141 atttcgcaaa cgcagaaaaa tgctcaatga tccgttccca gtcgggacga cattctccct
     1201 gtatccagat gttgaatgta gttccttcaa caataaaatc attatgacgc aattctgcag
     1261 atgaaataaa cgggctgtcg gcaagctcat gaaaatccat tggaaaaagc ctgaatgcat
     1321 ttttaccctg ctcggttgat tcctgatgcc atttgcatcc acaagccaat tgccatcctg
     1381 caggaacatt gatcaggact tcacatcctt cgttcattcg ttctttatcg taaatcaggc
     1441 aatttaccgg gttgacgtaa agctgcgtgg catcaacaaa agtggaacca gcgttaaggt
     1501 cgccggaaaa atactggtac gaaacaacaa tttcttccga tccggaaacc tgaattttcc
     1561 agctatcctt attcagtttt tcgaagggca aagcatgtcc ctgctcattt gtcacagcaa
     1621 aacgtcgtac atttttcgca aaatttccca actcgtaacg accggggcgc catgcgggta
     1681 attgcacttc tactgatttt cttttttcag caggtatccg aacaacaaca tcaataaaac
     1741 gatgttgagg ctggttgaat gaaagagagt acttaacgat catgttcaaa tttacgcatt
     1801 tcctcttccc gtgccagcag aaggttttgt attctgctcg atccattttc tcaaccaaat
     1861 caatggaagc acatttcccc tcgaataaac cgcaggatct attgtccgaa gcaaggcatt
     1921 ctttaccgaa atggattcat tttccaactt aaaacaggat gctaaaactt tcagaaacac
     1981 cttgtaatgt tcaacatctg ttcgtcccct caatagatca cgcagattcc ggatgtgtcg
     2041 ttcggcagta tcacgtttgt ttcgcatcac cagaattatc aattcctgaa ctctgtggta
     2101 caaatcatcg tgttctgata acttaccgga tagttcctgg tgcaaaacac gcgcatcctg
     2161 ccaattccca ttgaggagaa gcgccattcc tttgagtaaa ccaaaccgtg aaaacagaaa
     2221 aggatcgcgt tgcaaagcat gtccttccgt ttcaatcaac tcaagtgcat ccttgatttt
     2281 ttcgcatctg agcaaaacac cgatcaggtt atagacgatt tggtggacat taacttcagg
     2341 aatcgatttc cggtatttca aactttcccg aaaaagttca accgaatgat cgaactgctg
     2401 acgaaacgaa tattccaatg cgaggttaga caagatcaca gccagctccc tgctccaggg
     2461 aaactgaaaa tcatcgatgc tttctgcaag cgatctcatt tcttcaagct gttccattct
     2521 gcggtcactc acagcacgta aactttccgt tttgagacga aaataccgga ccactttatc
     2581 ctcttcttca ttggaaatgc ttgcattaat cttttgagcg agactttcag tatccgactc
     2641 ccccatccat ctgttgcacc acacatgaac actttcggcc tgtgcttttt tccgatcgaa
     2701 aaccaaatgc tcaagggaaa gaagctgttt gtttagttca gcaattgttt gaatattttc
     2761 cttttccgtt ttaccctggt tgacatatac actaagctcc attcgtatta ctcggatcaa
     2821 tccatcctga tcatgccgga tctccagttt ttttcggaca gatttcacct cgttcaaaaa
     2881 agtgctccat tcctttcggg aaagcaattc ctcgaggtaa aggatatcac gattgtgttc
     2941 atgaatttct ccacttcgtt ttagatagcg agcagataga aactccttca gtttatcgcg
     3001 taacaatcgt cgctcatgcc gtaaacgagc atcctttttc gacgaatagg gttctccaaa
     3061 aacctttctg tacacttcac tcgcttccgt ttttcgattt aggccttctg ctttccacaa
     3121 ctgaagcaag gtatttcttg cacaattttt caacacacca ccaaattcaa taatctcttt
     3181 atcagttaat aacatcaaat tttaaattat tttttcccca caaaatacaa attttccgtt
     3241 tggttgggac tataaaaagc agcaattttg cagaaacaaa aactgatgtc catgtttaga
     3301 atcctgcttg ccgcaacatt tctgattttt acttccgtag agggaactgc acaggtacgc
     3361 cagcttatgc ttgaaaaagc gaaacaaatg agcaccacca agcaacaatc cacgcaacct
     3421 gcagatacaa cccaaaaagc aactccgtcc aatgaacccg taaagcagga acaacaaagc
     3481 aatcctatcg gtttttccgg aggatcgaaa aaagatgtgc gtcctgttta cacgttcaac
     3541 gacaacatgc ttgttgagat taccaactat aaaaaagacg gaaaaaaaga tggtgatccg
     3601 gtgaaggttc gctacttatt cagcaaggaa gcctacctag gcagtgaaat gactatagaa
     3661 ggaaaaaaac cggaagacaa aaccgaatcc acttccattg ttgagttcgg gaaaaaccaa
     3721 atggtaatgc tatccaaaac caaagatggc aacagtggat ttgtgatgaa gtacgacagt
     3781 cagaaaatcc aggaaaaagc agcaaaggat accagttcag cacccaaatt cacaaagacc
     3841 ggacgcacaa aaacaattct cggttacctc agcgaagagt gggtttctac cgatgaaaaa
     3901 ggaaataaaa ctgaaatatg gatctccaat caggtgaaac tggatgtaag tggtgcttct
     3961 gcagcttttg ccggtgcaaa gggttctaaa tcggtaaaca cacaaggcta cccacaggga
     4021 gctatgcttg aaatgaacta ttacgaaaag aatggcgaaa aaacgacctg gatcgttctt
     4081 gagatcaatc tgaataaaac aacaactatc tctacagaag gctacacctt catgggattc
     4141 taaaaaaact caaccatgcg aacaacactt ttaacagtgg tcgtaatgac caccctgaat
     4201 tctgttgcct attctcaaac aaccgcaaac gacggcgact ggaaaaaaaa ctatgttgta
     4261 ttgaacaaca ctccgcaggc agaacatatg atacgcggtg gcgacattga taatcttggt
     4321 ttcggctggg aaaacaattt caatccattt tcaggaaaaa gcaccgaaac acatttttat
     4381 ccatggactc ctgatacaac atttgcaggc actgaccgca ttctgatcgg cagctcagtt
     4441 ggaaaatcag aaccaccatg cagcgcagat gggtattcgg gcgaatggaa tgcattgaaa
     4501 acgcaatttg gaaaaacaac acaagccatt aagatccctc tttctattcc cgcaaccacc
     4561 attgttaagg atgtcatgct gcaattgttt gttgatgatt tccaatcgcc tgttttttgt
     4621 tcctcctttc aggtactgct taacggaaaa cgcgctccct tcatagagaa tattatcaat
     4681 aaactaaatc aaagcggacc aattggaaaa ttgatcacca tcgaaattcc tgctgaatac
     4741 ttatccttat tcaaagagaa acaacttgaa attctaattg atgatccaac aacaggagct
     4801 cacgatgggt atgcaattga tttcgttaag gtactgatca atccaaaacc gggtgcattc
     4861 aaaaaataca accacactgt attggtaaaa gataatctga caggagatcc gcttaagggt
     4921 gtcattgtag aacttgcaaa cggaaaaatt gtacgcacca acgacaaggg agaagcgatt
     4981 ttgcaggatc tggtactggg ttatcacccc atccgattaa cgcattgtgg ttatcacgaa
     5041 gtgtttaccg gagttgatat tgaagaaaaa ccggaggctc cccagacatt tgtaatggaa
     5101 gtccagcagt gataagcaaa aggctatact taccaaacaa agcttaatcc actcataaac
     5161 aaggacaaac acccgtaact tcggttttgg aggtatcccg cagtattgtt actttcgtag
     5221 cccattcacg atcttaaaaa aacacagcat catgagttac gatcttattg ttctgggaag
     5281 cggtcccggt ggttatgttg ccgccattcg tgcttctcaa cttggactaa aaaccgctat
     5341 tgttgagcgc gaaagtcttg gtggaatctg ccttaattgg ggttgtatcc ctac
//
LOCUS       DAOMYG010000206         5380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5253_length_5380_cov_10.554742, whole genome shotgun sequence.
ACCESSION   DAOMYG010000206 DAOMYG010000000
VERSION     DAOMYG010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5253_length_5380_cov_10.554742"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /gene="hisG"
                     /locus_tag="PK610_07730"
     CDS             complement(<1..673)
                     /gene="hisG"
                     /locus_tag="PK610_07730"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015361230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRJ35863.1"
                     /translation="MQNNMIRLAIQKSGRLSEGSLELLNDCGISVSNGIGRLMTQASN
                     FPMEVLFLRDDDIPQYVQDGVADIGILGQNVVVEKERSIDEVLALGFSRCRLSLAIPK
                     NENYNGPRWFEGKNIATSYPVILQQFLDANKVKASIHEISGSVEIAPGIGLAEGICDI
                     VSSGSTLFSNGLKEVEVIFRSEAVLVANSSLSAEKKALLDKLTFRINAVRQSRNNKYV
                     LLNAPD"
     gene            734..883
                     /locus_tag="PK610_07735"
     CDS             734..883
                     /locus_tag="PK610_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35864.1"
                     /translation="MKSITSSLLNGNTTDDDGFCSCSWSKDGNGRSTGETEMSGFALF
                     DTMDS"
     gene            complement(938..1348)
                     /gene="rnpB"
                     /locus_tag="PK610_07740"
     ncRNA           complement(938..1348)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK610_07740"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1652..1933
                     /locus_tag="PK610_07745"
     CDS             1652..1933
                     /locus_tag="PK610_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35865.1"
                     /translation="MLVYIRTIETDLLNFKGTTMDEYNQLAGAIDNELGLLTSNCTMS
                     GQAHDELHKWLLPFIDSSDAFFDEEELSQKKEKLSALKLSFETFNTYFE"
     gene            complement(1999..5211)
                     /locus_tag="PK610_07750"
     CDS             complement(1999..5211)
                     /locus_tag="PK610_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRJ35866.1"
                     /translation="MRKFYLLLALLLMGVVPNALRAQTVYSEYQDGKIWFKIKEDVRL
                     NLPGFRMEQEALVNYNNLDLTTLNFLRPLSAQHQITKLSRPYWMVKNDLNLSNVFLLE
                     FNDFSNVDVIMNKIKQSGMVEYAERVPLLKHFLTPNDPSFSSSTQWGLFQINAQQAWN
                     ISTGSPTVVVAIVDDAIQTNHPDLTAVIYTNNGEIAGDGIDNDGNGYIDDRNGFDVAD
                     NDNNPNPPTNAFDHGTHVAGIAGARSNNSVGVASIGYNIRLLPVKATNSSSSVTHGYD
                     GVVYAVNAGAKIINMSWGGTGSSTTASNIINWASNQGVVLVAAAGNDNDNVLHYPAAY
                     TPCIAVASTTTGDVRSSFSCFGTWVDVAAPGSSIYSTTVGSSYGYKQGTSMASPMVAG
                     LAGLIKSVNPSMTATDIRNCILNTAVNINAANPSYVGQLGSGRIDAFAAVNCAAATLN
                     NPPVADFVANNTTISAGASVNFTNLSIYNPTTYSWTFAGGTPGTSTAANPTNIVYSTP
                     GTYTVTLTATNANGSDVETKTAYITVVPTSGCDRPNWSNLSQNGGTWTGTQYYTGAAV
                     GQDGWVNGKNVYNDREKAAYFNVSSNTQGYIEGCWIAFGEAYSATPSKIVPVKVYNRT
                     GANPGTLLATYNLTMGEIMADVQGNFWTEILFPTPIAVPASREFFISVDVNNLSWTAG
                     VKDTLSIISNTNGQTTPSAIWERQTNNNWYQYGTAGTWNLTASLYIIPFLTSDPASVS
                     ITSSATSVCAGSSINFDATGSNVGTAKLWRLNGGSPNGSNNMQVTSFYNTAGSYYAKF
                     EMLGGGCNLYKADSIQITVNPVPNLSITSSDDTICPAGSSQLNVSSAATSYVWSPPTG
                     LSSTNISNPVATPANTTTYTVTGTLGPCSKSGDVTVVVDNIPPVAEFLYSVPTCINAP
                     VTFNGAVSDFATAYNWTFSGGSIASSVSPGPSVTYAAAGFYPVTLQVTSECGFTDDTT
                     ITVQVIDCTISTGDILEDGGISAFIDNNNTIQVTFESASLDKYNLTLVNAMGQIVHTS
                     QEEFPAAGRVVRIPLEDKAQGIYFLRVDNGQRGVVFRFFR"
BASE COUNT         1576 a         1188 c         1143 g         1473 t
ORIGIN      
        1 cgtcgggcgc attgagtaaa acgtatttat tgttccgcga ctgacgaacc gcattgatac
       61 gaaaagtcaa tttatcaagc aatgctttct tctccgcact cagcgatgaa ttggcaacca
      121 aaactgcttc cgaacgaaaa ataacttcaa cttcttttaa tccgttactg aacaatgtgg
      181 aaccactgct tacgatatcg caaattccct ctgccaaacc aataccaggt gcaatctcta
      241 ctgatccgct gatttcgtga atggaagcct taactttatt tgcatcgagg aattgctgca
      301 gaatgacagg ataagaagta gcaatgtttt tcccttcaaa ccaccgtggt ccattatagt
      361 tttcattctt cggaatagcc aaggacaaac gacaacgcga aaatccaagc gccaggactt
      421 catcgataga gcgttctttt tctaccacca cattttgtcc gagaattcca atgtctgcta
      481 ccccatcctg aacatactgg ggaatatcat catctctcag aaacaaaact tccattggga
      541 aattggatgc ttgtgtcatt aacctgccaa ttccgttgga aacggaaata ccgcagtcgt
      601 tgaggagttc aagtgaacct tcggataatc gtcctgattt ttggatcgct aatcggatca
      661 tgttgttttg cattggagtt cgaaattagt caaaataaaa aaggcctcca ccgggagacc
      721 tctgccaaat aagatgaaat ccatcacctc cagtctcctg aatggaaata cgacagatga
      781 tgatggattt tgttcatgtt catggagtaa agatggtaac ggaagatcaa ccggcgaaac
      841 tgaaatgagc ggatttgccc tatttgatac gatggatagt taaatccggg aaatacaaga
      901 gtcaggaaag tcacccttcc tcctcttgca taacctgaat taaaaaaaac ctgtaagccg
      961 ggttctgtat tcgtttgcac gaattctcat cattgatctg ggatcgcatt cacatacgac
     1021 ctcaagcagc ctacccatca cagcgacctg ccgaagcaga tacggaaagc gagccgccct
     1081 ccctccggaa aaccggaatc tgtgacctac atggcctttc agcccgcaag gtttacccat
     1141 ccggaatgtc gccacccgga ccgtgagctc ttacctcaca ttttcaccct tacccttctg
     1201 aattgcttca gaatggcggt atactttctg ttgcactgtc tgttcctgca aagtcaccct
     1261 tacaggacct acccgttagg tagtgcggtg ctctgtgctg cccggacttt cctctgtaat
     1321 tgcttacagc gatgagacgg tttcggttgc aaatgtagtg caatttatgg caaatgcatt
     1381 ttccgttcgc agcttatcat ttttggagta tgacaactat cacaaaacgt cttaatcgaa
     1441 ctatgtacct tgcagaaaat ttcatccaat gaaaatccaa ctcatcctat tgctttttgc
     1501 cggaagttta cttgcagcat gttaagatca ttcaactgaa aaatccaaca ctattgctga
     1561 caccgacacc atacaggaac agcaccagca ccatgatcat gaagaaggaa ttcaattgaa
     1621 cgatggtaag aaatgggtcg tagaacccaa aatgcttgtt tacatccgta ctattgaaac
     1681 cgatcttcta aatttcaagg gaacaaccat ggatgaatac aaccaactgg caggggcaat
     1741 tgataatgaa ttgggattgc tcaccagtaa ttgcacgatg agtggtcagg ctcatgatga
     1801 attgcataaa tggcttcttc cttttatcga tagttccgat gctttttttg atgaagaaga
     1861 gctgagtcag aaaaaagaaa aactttctgc attgaaatta tcgttcgaaa cattcaacac
     1921 ctattttgaa taattcagat ttacatcaaa aaaaagcccc cttctcagga agggggcttt
     1981 tcattttatt gaaactgttt atcggaaaaa cctgaatacc actccgcgct gaccattatc
     2041 cacacgtaag aaataaattc cctgtgcttt atcttcaagc ggaatgcgaa ccactcttcc
     2101 tgctgcagga aactcttcct gcgaagtatg cacgatttgt cccattgcat tcacaagtgt
     2161 aagattatac ttatcaagtg aagctgattc aaacgttacc tgaatggtat tgttgttgtc
     2221 gatgaatgca ctgattcctc catcttcaag aatatcgcct gtagaaatag tacagtcgat
     2281 aacctgaacc gtgatggtgg tatcgtcagt aaatccacac tcggaagtaa cttgcaatgt
     2341 aacgggataa aatccggctg cagcatacgt tactgaaggt cccggtgaga cagatgacgc
     2401 gatactacca ccggagaatg tccagttgta ggcagtagca aaatcagaaa ccgcaccatt
     2461 aaacgtaact ggtgcgttga tacaggttgg tacactgtaa aggaattctg ccactggagg
     2521 aatattatct actaccactg ttacatctcc tgatttagag caaggaccga gtgtaccggt
     2581 tacggtatag gttgtagtat tggcaggtgt tgcaacagga ttagagatat tggtagaact
     2641 caaacctgtt ggaggtgacc aaacataact cgttgcagcc gaagaaacat tcagttgcga
     2701 tgaaccggca ggacagatgg tatcgtcgga tgaggtgatg gaaagattcg gaacaggatt
     2761 aacagtgatt tgtatggaat ctgctttgta gagattacaa cctccaccca gcatttcaaa
     2821 tttagcatag tacgatcctg ctgtattgta gaagctggta acctgcatat tgttcgatcc
     2881 gtttggacta ccaccgttca atcgccatag ttttgctgtt ccaacatttg atccggttgc
     2941 atcaaaattg atcgagcttc ctgcacaaac cgaagttgcg ctggatgtaa tactcaccga
     3001 tgcaggatcg cttgtgagga acggaatgat ataaagcgaa gcagtaagat tccatgttcc
     3061 tgcagttccg tactggtacc agttgttatt ggtctgacgt tcccagatag cagatggtgt
     3121 tgtttgtccg tttgtgttgg agataatcga caaggtatct tttacaccgg ctgtccatga
     3181 aaggttgttt acatcaacag agataaaaaa ttcacgactg gcaggaactg caattggtgt
     3241 tggaaataaa atttctgtcc agaaattccc ctgcacgtcc gccatgattt cacccatggt
     3301 gagattgtaa gttgcaagaa gtgttcccgg atttgctcct gtacggttgt acacttttac
     3361 aggtacaatt ttggatggag ttgcagaata cgcttcacca aatgcaatcc agcatccttc
     3421 gatgtaaccc tgcgtatttg acgaaacatt gaaataagct gctttttcgc gatcgttgta
     3481 aacgttcttt ccgtttaccc aaccatcctg accaacagca gctcctgtgt agtattgcgt
     3541 acctgtccat gttccgccat tctgcgaaag gttgctccag ttcggacgat cacatcctga
     3601 agttggaacc actgtgatat aagcagtttt tgtttccacg tctgaaccat ttgcattggt
     3661 tgcagtaagt gttaccgtat aagttcccgg agtactgtaa acaatattgg tggggttggc
     3721 ggcagtagat gtaccgggag taccaccggc gaatgtccag ctataggttg taggattata
     3781 aatactgagg ttcgtaaaat tcaccgaagc accagcagaa atggttgtgt tgtttgcaac
     3841 gaaatctgca acaggaggat tgttaagcgt agcagctgca caattcacag cagcaaaggc
     3901 atcaatacga ccggaaccta attgtccgac atacgaagga tttgctgcat tgatatttac
     3961 tgcagtattc agaatacaat tccggatatc ggttgcagtc atggaaggat tcaccgattt
     4021 aatcaaacct gcgagtcctg caaccattgg tgatgccatt gatgttccct gtttgtagcc
     4081 gtagctgctt ccaacagtag tagaataaat agaagatccc ggagcagcaa catctaccca
     4141 tgtaccaaaa caactgaagg aagaacgcac atcacccgtt gtagttgaag caacggcaat
     4201 acaaggcgta taggcagcag gataatgcaa tacattatca ttatcgttac cggcagcagc
     4261 tacaagaacc acaccttgat tagaggccca attgatgata ttggaagctg ttgttgaaga
     4321 gccggttcca ccccatgaca tattgatgat ttttgcacct gcatttaccg catacaccac
     4381 accatcgtaa ccgtgagtaa cagaggatga agagttggtt gctttaacag gaagaagacg
     4441 aatgttgtat cctattgaag ctactccaac tgaattgttc gaccgtgcac ctgcaatacc
     4501 tgcaacgtgt gtaccatgat cgaatgcgtt ggttggagga ttgggattgt tatcattatc
     4561 ggcaacatca aaaccattac ggtcgtcgat atatccatta ccatcgttat cgataccatc
     4621 acctgcaatt tcaccattgt tggtataaat tactgcggta agatcggggt gattggtttg
     4681 aatggcatca tcaacaattg caactacaac agttggacta cctgtagaaa tattccaggc
     4741 ctgctgagca ttgatttgaa acaatcccca ttgagtggaa gaagaaaaac tcggatcgtt
     4801 gggagttaaa aaatgcttga gtaagggaac gcgctcggca tattcgacca ttccactttg
     4861 cttgatcttg ttcatgatca catcaacatt tgaaaaatca ttgaactcca gcaaaaacac
     4921 attcgacaga ttcaaatcat ttttcaccat ccaatacgga cgagataatt tagtgatctg
     4981 gtgttgtgcg ctgagcggac gaaggaaatt gagcgttgta agatcaagat tgttgtaatt
     5041 caccaatgcc tcctgttcca tcctgaaacc cggaagattg agacgcacat cttctttgat
     5101 cttgaaccag atctttccat cctgatactc ggagtacacc gtttgtgcac gcaaagcatt
     5161 cggaacgact cccataagga gcaatgcaag tagtaggtaa aattttctca tggttttttg
     5221 gatttagccg cctcaatata cgaaggcgaa ttgcaatttg gagccctata ggccgcttat
     5281 ttaggcaacg taaaacagaa aatctgaacc gttaaaaaaa actccttttt gctcaaaacc
     5341 aaaacaatat cccttcaggc aaaacgtctt ggataaaaga
//
LOCUS       DAOMYG010000207         5376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5258_length_5376_cov_10.803232, whole genome shotgun sequence.
ACCESSION   DAOMYG010000207 DAOMYG010000000
VERSION     DAOMYG010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5258_length_5376_cov_10.803232"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="PK610_07755"
     CDS             <1..1150
                     /locus_tag="PK610_07755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ35867.1"
                     /translation="YDESLWDWEYISSSNEPLTPGKGFEVWLDDDGSYTSFSETIIDS
                     RGTPNRGNISRAVTVDNDGWNLIGNPYAAHIDWTTFRSTSGIFMYDHFMYWDEAEDDY
                     SFGGPGDEIAPHQGFWIEASSAGNVIFRETNKTTSNSSFFRSTQPGFFKLRLKSDGET
                     NFTSNTNFVFEDGLSKAYEQGSDVTFKKVPHPNAPSLYSLSTEGKRLRLNKMEAENNL
                     VLPLGFAVGVSGFYTISANELGLAQDAGFNCVVLEDKHTKTFTDLIEGSYRFYADGAS
                     DERFVLHLSKDNSACNRNLVSSASSELQGISITHTEQGIFVNYDLEDEMNSVISVTNL
                     LGQEIVSARSISISKGNIRVALPESFHGMFIVSVRQGDKMVTRKFFAP"
     gene            1282..1497
                     /locus_tag="PK610_07760"
     CDS             1282..1497
                     /locus_tag="PK610_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35868.1"
                     /translation="MKKRLYLILLLAILTVFAQESMALGMGMGMGPPNPPCGVAPFPP
                     CPVPLDGGLIFLAAAGTIYAGRRLGKK"
     gene            complement(1579..2853)
                     /gene="glyA"
                     /locus_tag="PK610_07765"
     CDS             complement(1579..2853)
                     /gene="glyA"
                     /locus_tag="PK610_07765"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRJ35869.1"
                     /translation="MKRDETIFHLIEEEKQRQTRGIELIASENFVSEQVMQAMGSVLT
                     NKYAEGLPSKRYYGGCEVVDKVEQLAIDRLKQLFGAEWANVQPHSGAQANAAVMLAVL
                     NPGDAILGFDLSHGGHLTHGSPVNFSGKLYRPHFYGVEETTGTINYDKVEATAKECKP
                     KLLICGASAYSRDWDYARLRSIADSVGALLLADISHPAGLIAAGLLNNPMPHCHIVTS
                     TTHKTLRGPRGGIIMMGQDFDNPWGHKTPKGEIKKMSALLDAAVFPGTQGGPLEHVIA
                     AKAVAFGEALSPEFKAYGQQVMKNAAVMADEFIKRGYKVISGGTSNHSMLIDLRSKNL
                     TGKAAENALGLADITVNKNMVPFDDKSPFVTSGIRVGTPAITSRGVKENEIAKIVELI
                     DKVLMNIGNESAIAEVRKEVNAMMSGKSLFHH"
     gene            complement(3102..4421)
                     /locus_tag="PK610_07770"
     CDS             complement(3102..4421)
                     /locus_tag="PK610_07770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRJ35870.1"
                     /translation="MKLRTKPFLLLFAVTLLIRLALLIAVQVSDPELHMLHDSYGYAS
                     IGENFFEHGVWSQSEEAPFYPDVFRTPVYPLLIGSIVQLGGDLLALMLVQILISLLTL
                     YFILRAVDLLSNGNTKVMLIIGWLYALDVPSIVMGNLVMTENVFAMLIVIACYFLFLP
                     LRHKSTKNSLSFFYAGIFLGLAILTRPVALYLPVLLIPFLLWKFRMQALWLVVPVMLL
                     TGSWYARNYAVHRQVFYSEIGNFNLLYFQAADLFAKEHGLTLDQARAHLYEQVQNEME
                     VIPREDPVLFYEMCGQKAKGIIASHPGGFLKNALKAQSNLFFRPLRGYLDYQLGVADV
                     YSASSPYGRSSFLGLFAEGSHGLTSLTIPLQLLMNLAVYAALLLSLFRMFRTDRTLFW
                     LALILIGYFVLVCSGPEIDARFRVAVLPLLLVFTTKGFSLPRREGIV"
     gene            complement(4418..>5376)
                     /locus_tag="PK610_07775"
     CDS             complement(4418..>5376)
                     /locus_tag="PK610_07775"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HRJ35871.1"
                     /translation="ISDEAQHFPELFSFIQVISDEVGKKGEFVLTGSQHFLLSQRISQ
                     SLAGRVFVTHLLPCTISEANRFEHWRNSPNAAMYSGFYPRIHESRIEPHLFYPSYIQT
                     YIERDVRQIINITRLGDFQRFMRLLAGRVGQVMNYSALANEVGVDMKTIKAWTQLLEA
                     SFVVFLLQPHHANYNKRIVKSPKLYFYDTGLVCNLLGVQSQEQLDAHWVRGSLFENMV
                     IADIKKYFHHHHRQLQLYFWRDSNGNEVDLLIEHSDGLDIAEIKSSTSVNASFFKGLD
                     YYSGLNQSVKRSFLIYGGEESRTRGRHLILPWHIAAQQIAEV"
BASE COUNT         1491 a         1313 c         1203 g         1369 t
ORIGIN      
        1 gtatgatgaa tcactatggg actgggaata tatttcctct tcaaacgaac ccttaactcc
       61 gggtaaaggt tttgaagtat ggttggatga tgatggttcc tatactagtt tttctgaaac
      121 aatcattgac agccgaggca ctccaaaccg gggtaatatt tcccgcgctg taactgtaga
      181 taatgacggg tggaacctta ttgggaaccc ttatgccgca catatagatt ggactacatt
      241 taggtctaca tcaggcattt tcatgtatga tcatttcatg tactgggatg aagcggaaga
      301 tgattattct tttggtgggc cgggcgatga aatcgctcct caccagggtt tttggattga
      361 agctagctca gcaggaaatg taattttcag agaaaccaac aagaccacat ccaactcaag
      421 cttcttccgc agcacgcagc ccggattctt taaactgcgc cttaaaagcg atggtgaaac
      481 aaacttcact tcgaatacca attttgtgtt cgaagacggc ctgagcaaag cctacgaaca
      541 aggaagcgac gttaccttca agaaagttcc gcaccccaat gctccctcgc tatacagcct
      601 cagcacagaa ggcaagcgtt tgcgtctgaa caaaatggaa gctgaaaaca atctcgttct
      661 gccactcggt tttgcagttg gagttagcgg tttctacacc atcagcgcaa atgaactcgg
      721 actcgcccag gatgcaggat tcaactgtgt ggtactggaa gacaaacaca ccaagacctt
      781 caccgacctt attgaaggca gttaccgttt ctatgcagat ggagcatccg atgagcgttt
      841 cgttctccac ctcagcaaag acaacagcgc ctgcaaccgt aatttggtct cttcagcctc
      901 atctgaatta caaggcatca gcatcaccca caccgagcaa ggcatcttcg tgaactacga
      961 tcttgaggat gaaatgaact ctgttatcag cgtcaccaac ctcctcggtc aggaaatcgt
     1021 tagcgcccgc agcatcagca tctccaaagg aaatattcgt gtggcactcc cagagagctt
     1081 ccacggtatg ttcattgtaa gcgtacgcca gggagataag atggtgacga ggaaattctt
     1141 tgctccgtga ggagaaaaga gttattagtt atgagatatg agttatgagt agggctatct
     1201 attaccctac ttttaactcc taactcttaa ctcttacctt ctaacttttt cctcctaact
     1261 aatttttcta tatttgtaat catgaaaaaa cgcctttacc tcatcctgct tctcgctatt
     1321 ctgacggtct tcgctcaaga atcaatggca ttaggtatgg gtatgggtat gggtccaccg
     1381 aatcctcctt gtggtgttgc tccatttcct ccgtgtccgg ttccactgga tggtggattg
     1441 atcttcctcg ctgcggcagg aacgatttat gcaggacgga gattagggaa aaagtaaatt
     1501 tttttttgaa ccacaaagac actcattgca cttagatgca cttagaaatt cttagtgttc
     1561 cttagtgggc tcagtgcctc agtggtgaaa aagagacttc ccactcatca tcgcattcac
     1621 ttcttttctc acttccgcaa ttgcactttc gttcccgatg ttcatgagta ctttatcgat
     1681 aagctcaacg atttttgcga tctcgttttc ctttacaccg cgtgatgtaa ttgcaggagt
     1741 acccacacga attccgctgg taacgaatgg tgatttatcg tcgaagggaa ccatgttttt
     1801 attgacggta atgtctgcaa gtccgagtgc gttttctgcg gctttaccgg tgaggttttt
     1861 agagcgcaga tcgatgagca tgctgtgatt ggaagttcct ccgctgatga ctttgtatcc
     1921 gcgtttaatg aattcatccg ccatgactgc agcatttttc atcacctgct gaccatacgc
     1981 cttgaactcg ggcgacagtg cttcaccaaa agccaccgcc ttggcagcga tcacgtgctc
     2041 gagtggtccg ccctgtgtac cggggaacac cgcagcatcc aataaggccg acattttctt
     2101 gatctctccc ttgggtgttt tgtgtcccca tgggttatcg aaatcctgtc ccatcatgat
     2161 gataccgcca cgtggtccgc gcagtgtttt gtgggtggtc gacgttacaa tatggcaatg
     2221 cggcatcggg ttgttgagca atcctgcagc aatgagtccg gccgggtgag aaatatccgc
     2281 caaaagcagc gcacccaccg aatcggcaat ggaacgcaga cgtgcataat cccaatcgcg
     2341 cgagtaagct gaggctccgc aaatgagaag ttttggcttg cattctttgg cagtggcctc
     2401 cactttatcg taattgatgg ttccggtagt ttcttccacg ccgtagaaat gcggacggta
     2461 aagtttaccg gagaaattca ccggagaacc gtgggtaagg tgtccgccat gagaaaggtc
     2521 gaaaccaaga atcgcatcgc ccggattaag caccgccagc atcaccgctg cattggcctg
     2581 cgccccggag tgcggctgta cattggccca ttctgcacca aagagctgct tgagacgatc
     2641 gatcgccaat tgttccactt tgtccactac ctcgcaacca ccatagtacc gcttggaagg
     2701 gagtccctcg gcgtacttgt tcgtcagcac cgagcccata gcctgcatga cttgttcgct
     2761 cacaaaattt tcggaagcga tcagctcaat accgcgggtt tggcgttgtt tttcttcttc
     2821 gatgagatgg aaaatggttt cgtcgcgttt catggttgta ggttgtgggt gcaaatgtag
     2881 aaatttcacc actaagtcac taagaacact taggtacact aagtcttagt gcttcttagt
     2941 gcaaaaaaat cactactaag tcactaagaa cacttaggtt cactaaatct tagtgattct
     3001 tagtgcaatt agtgccttag tggtgaaaac ctccttccct ttgcggttaa acccctcctt
     3061 gatccttcgt agcaacagcg aagaagggcc tccctttgcg gttaaacaat cccttccctc
     3121 cgcggtaaag aaaatccctt agtggtaaaa acaagcaaca aaggcaacac cgccacccgg
     3181 aaccgcgcat cgatctccgg tccggagcag accagcacga aataaccaat aaggatcagc
     3241 gccagccaga acagcgtccg atctgttctg aacatccgga aaagagaaag cagcaaggcc
     3301 gcataaacag caagattcat gagtagctgc aggggaatag taagtgatgt cagcccatga
     3361 ctcccttcgg cgaatagtcc gagaaaggag cttcgtccgt aaggagaact tgcgctataa
     3421 acatcggcaa cacccaattg gtaatcgagg taaccccgca gcgggcgaaa aaataaatta
     3481 ctctgtgcct tcaacgcatt tttcaaaaat cctccagggt gagaggcaat gattcccttt
     3541 gctttttgtc cgcacatctc gtaaaacaag accggatcct cccttggaat cacctccatt
     3601 tcgttttgca cctgttcgta caaatgcgcc cgtgcctgat cgagtgtgag tccgtgttcc
     3661 ttcgcaaaaa gatcagccgc ctggaaatac agtaagttaa aattcccgat ttccgaatag
     3721 aacacctgtc gatgcaccgc ataattccgt gcataccagc taccggtcaa gagcattacc
     3781 ggaaccacca accaaagggc ctgcattctg aatttccaaa gcaaaaaagg aatcagcaac
     3841 accggcaaat acagcgcaac cggtcgggtg agaatagcga gtccgaggaa gattccggcg
     3901 taaaaaaaac ttagtgaatt cttagtgctc ttgtgtctta gtggtaaaaa aagaaaatag
     3961 caagcgatta cgatcaacat cgcaaacaca ttctccgtca tcaccagatt ccccattacg
     4021 atggagggca catccaatgc atacagccaa ccgatgatca gcatcacttt ggtatttcca
     4081 ttggagagaa gatctactgc acggagaata aaataaaggg tgagaagaga aatcaggatt
     4141 tgtaccagca ttagtgcaag taaatcgcca ccgagttgaa cgatgctacc gatcaaaagc
     4201 ggatacaccg gcgtgcgaaa aacatccgga taaaacggcg cctcctccga ttgcgaccat
     4261 accccgtgct caaaaaagtt ctcgccaatc gacgcatacc cgtacgaatc gtgcaacatg
     4321 tgcaattccg gatcgctgac ttgtaccgca atgagcaatg ccagtcggat caggagggtg
     4381 acagcaaaga ggagtaaaaa gggctttgtg cgtaatttca tacctctgct atttgttgtg
     4441 ccgcaatatg ccagggaaga attaagtgcc gacctcgtgt tcttgattct tctccaccgt
     4501 aaatcagaaa ggatcgtttc accgattgat ttaacccgga ataataatcg agtcctttaa
     4561 agaacgatgc attcaccgag gtacttgatt ttatttctgc gatatctaaa ccgtccgaat
     4621 gttcaatgag aagatctacc tcattaccat tactatcccg ccaaaaataa agctgaagtt
     4681 gccgatgatg atgatggaaa tattttttga tgtcggcaat aaccatattc tcaaacaatg
     4741 agccacgcac ccaatgcgca tcgagttgtt cctgcgactg tacaccgagt aaattgcaaa
     4801 caagacccgt atcgtagaaa tagagttttg gcgatttaac aatccgcttg ttgtaattgg
     4861 catgatgtgg ttgcaagagg aaaacaacga aggaagcttc cagtaattgc gtccatgctt
     4921 ttattgtttt catatccacc cccacttcat tggcaagagc ggaataattc atcacctgac
     4981 caaccctccc cgcaagcaga cgcataaaac gctgaaaatc gcctagacgt gtaatgttaa
     5041 tgatttgccg aacgtcccgc tcgatatagg tttggatata tgatggataa aacaaatgtg
     5101 gttcaattct actctcgtga atacgcggat aaaatccgga atacatggct gcattgggcg
     5161 aattgcgcca gtgctcaaaa cgattcgctt cagaaattgt acagggtaaa agatgcgtta
     5221 caaaaacacg tccggcaaga ctttgcgaaa tacgttgaga aagcaaaaag tgctgcgaac
     5281 ccgtgagaac aaattcaccc tttttcccaa cctcatcgct gatcacctga ataaaggaaa
     5341 acaattcggg aaaatgctgc gcttcatcgc taataa
//
LOCUS       DAOMYG010000208         5366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5267_length_5366_cov_10.036340, whole genome shotgun sequence.
ACCESSION   DAOMYG010000208 DAOMYG010000000
VERSION     DAOMYG010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5267_length_5366_cov_10.036340"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="PK610_07780"
     CDS             complement(<1..1325)
                     /locus_tag="PK610_07780"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRJ35872.1"
                     /translation="MAIGFPAKYSQTVSLKKGDVLSLVSAVEQCLKELQWEITSSDLR
                     EIRANTNFSLSSWGELFTIQFAGNDMILTSSCKGTQLIDWGKNRRNIKKFLELWNKHN
                     QTTLEMFSGYSASENFETEHQVENSVWSGFLPSKGFFVTPSLIIINLLVFVAMVVSGV
                     HIMEPASLDLVNWGANFRPLTSGGEWWRLFTCCFIHIGILHVLMNMYALMYVGLYLER
                     IIGSVPFLILYLLTGVVSSVASLWWHDFTISAGASGAVFGMYGVFLALLLSKSIDEEI
                     RKALLPSIGIFIGYNLLAGAKGGIDNAAHIGGLLSGFVSGFALIPALRSNSSSGLVSK
                     SLIIISVVFMAFSYFVATNLSNDIPKYEKLMEEFSIHESAALKPYSLLENEGSPEDII
                     AAFSGDGMKEWLECKRIITEAIELDLPEHIRDVHGADRPGRRSGCVAGGV"
     gene            complement(1335..2297)
                     /locus_tag="PK610_07785"
     CDS             complement(1335..2297)
                     /locus_tag="PK610_07785"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HRJ35873.1"
                     /translation="MKSKNKDQFRTVFEPVEANRKITHKESVFFIGSCFSTHVSGRMK
                     NAGFQITANPFGVLFNPVSVSRALRMIGTLKKFRKQDFFHHDGLWHHFHLHGELSKTD
                     LDSAYSSANEMIENSNRALMSAGTLVITLGTAWVHEEIDTERIVANCHKLPGKQFRKR
                     KLGVNEIISDFSRLLEHLFNVNPGIRIIFTISPVRHLSDGFVENQWSKSTLNIAIHEL
                     IRRFEQTEYFPAYELIIDDLRDYRFYNSDMIHPNDQAADYVWNIFRKTYFDEYTEKLA
                     SEAEAYHLSIQHRAIHPDTEAHRKFIAQRTKEGDRIKKLLPWLV"
     gene            complement(2294..3121)
                     /locus_tag="PK610_07790"
     CDS             complement(2294..3121)
                     /locus_tag="PK610_07790"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ35874.1"
                     /translation="MKANSVRKISIIGCGWLGKPLAIHLRNAGYEVMATNTSGRSDSE
                     LDSSGVKICSLKATSEDLSFSDDAVFFCDVLIINFPPARNEEKASQHPLQIKLIAEQI
                     RKNKIQRVLFVSSTSVYPDLNTLVDENCEAPPVKASGKALLDAERILRDESGSKVTLI
                     RFAGLIGYDRLPARFLAGKKELKDGQSPVNLIHRDDCIRIIEKIISRDAFGEIYNGVC
                     DQHPLRADFYTRAAMNAGLPLPEFLDLPSDNWKIVSGEKLKNHLQYNYIHPDPMLLI"
     gene            complement(3118..3843)
                     /locus_tag="PK610_07795"
     CDS             complement(3118..3843)
                     /locus_tag="PK610_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ35875.1"
                     /translation="MIVQTKSLYVGYAHASGDCALLEDLNLSIDESEIIALLGANGSG
                     KSTLLRSLAGMKSILAGQLFINGKDTRTFSGEEWSRNVGMVLARTPDTGSLDVYGLIA
                     LGRIPYTGLFGRLKDDDHRIIAEVMEQMDLSDFAWRKVAELSDGERQRVMIARALAQQ
                     TPLVVLDEPTAFLDLSMRRKMAAKLRELAKEQKHSFVLSTHEIDLALNSATRFWIIGR
                     DRAFYDIQSSVEAEAIIQSEFGV"
     gene            complement(3840..4898)
                     /locus_tag="PK610_07800"
     CDS             complement(3840..4898)
                     /locus_tag="PK610_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRJ35876.1"
                     /translation="MNRLSQQYTAQRRKSFLLIFVLACAVPVLFLLDLFLGSVSIPFS
                     EVVTVLTGGTSEKEAWNYIILDTRLPQSLTALSAGAALACGGLLLQTFFRNPLAGPDV
                     LGISSGAMLGVAIVFMGGDLLGMHFSGSLSGFLQVFSAMTGAILVLVLVVFLSGVSRD
                     GVTVIIGGLMIAYLAGALVNLLQHAAAREGLQQFVFWGFGSFSSMDLTSSLRFSALII
                     VLLIPSFFLTRSLNAWVLGPVYAQSVGVSLKKFRLGIIISSGMLTAIVTAYCGPIAFI
                     GIAVPHLVRWVIRTEDHSLLLPAVVLLGAALSLFCSLITRLPVFETPLPLNAVTSLVG
                     APVVLIVILKQRHKGGFA"
     gene            complement(4895..>5366)
                     /locus_tag="PK610_07805"
     CDS             complement(4895..>5366)
                     /locus_tag="PK610_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ35877.1"
                     /translation="NEYIALIKQADHSGDSPTVFSGKAYNGEWHVPGGKSFAARLLVD
                     AGGNYLWRDDAHNNSLTCNMEVVMDKAMNAEWWVIVGACNDDFNLEQLRKEDPRYAEF
                     DAFKNGKVIFCNTNKCDYFGAAVAEPQVLLKDLLSFFQPKIVSGHTPVYFQQLK"
BASE COUNT         1716 a         1259 c          979 g         1412 t
ORIGIN      
        1 acgccgccgg caacgcagcc actccggcga cccggccggt cagcaccgtg gacatcacgg
       61 atatgttcag gtaaatccaa ttcaatggct tctgtaatta ttcgtttaca ttccagccac
      121 tccttcattc catcgccgga aaaagcagca ataatatctt ccggagatcc ttcattttcc
      181 agaagtgaat aaggtttcaa tgctgccgat tcatgaatgg aaaattcttc cattagtttt
      241 tcatacttcg ggatgtcgtt tgaaagatta gttgcaacaa aataagagaa tgccataaat
      301 acgactgaaa taattatcag tgattttgaa accagtcccg acgatgaatt cgatcttaaa
      361 gcgggaatta aggcgaaccc ggaaacaaat ccgcttagta atcccccaat atgagcagca
      421 ttgtcgatcc cgccttttgc tcctgccagc agattgtaac caatgaaaat tccgatactc
      481 ggcaataagg cttttcgtat ttcttcatca atacttttag acagcagcaa agcaagaaac
      541 acaccgtaca taccaaacac agcacctgaa gcacctgcac tgatggtgaa atcatgccac
      601 cacaaactgg caacactgct cacaactccg gttaacagat ataatatcaa aaatggaacc
      661 gatccaataa tccgctcgag gtaaagccca acatacatca atgcatacat gttcatcaac
      721 acatggagaa ttccgatatg gataaaacaa caggtaaata aacgccacca ctcaccaccg
      781 gaagtcagag gacgaaaatt cgctccccag ttgaccagat ccaaactcgc cggctccatg
      841 atatgtacac cggaaaccac catggcaaca aagaccaaaa gattgatgat gataagcgaa
      901 ggagtaacga aaaacccttt gcttggtaaa aatccagacc atacagaatt ttcaacttga
      961 tgttccgttt caaaattttc actcgctgaa tagccggaaa acatttctaa agttgtctgg
     1021 ttgtgtttat tccataactc caaaaacttt ttgatatttc ttctattttt tccccaatca
     1081 attaactgag tccccttgca ggaacttgta aggatcatat cgtttccggc aaactgtata
     1141 gtgaaaagtt caccccagga actaagagag aaatttgtat tcgcacgaat ttcacgcaaa
     1201 tcggaagatg tgatttccca ttgaagctcc ttcaagcatt gttcaacagc agaaacaagt
     1261 gaaagaacat cacctttctt cagactaact gtttgcgaat actttgctgg aaatcctatt
     1321 gccataattt cagattatac caaccaaggt aataactttt taattctgtc cccttctttg
     1381 gtgcgctgtg caataaattt tctgtgagct tcggtatccg gatgaatagc acggtgttga
     1441 atcgacaagt ggtacgcttc agcttctgag gccaattttt cggtgtattc gtcaaaatat
     1501 gtttttctga atatattcca aacataatcg gcagcctgat cattggggtg aatcatatcg
     1561 ctgttgtaaa atcgataatc ccgcaaatca tcaatgatca attcataagc aggaaaatat
     1621 tccgtttgtt caaaccgtcg gatcagttcg tgaatggcaa tattgagcgt gcttttgctc
     1681 cattgatttt ccacgaatcc atcacttagg tggcgaacag gactaatagt aaaaatgata
     1741 cggatgccgg gatttacatt aaacaaatgt tccagcaaac gggaaaaatc actgatgatt
     1801 tcattcacac ccagtttgcg tttgcgaaat tgtttaccag gtaatttgtg acagtttgca
     1861 acaatccttt cggtatctat ttcttcatgc acccacgctg ttcccagagt aataacaaga
     1921 gtaccggcgc tcatcaatgc gcgattcgaa ttctcgatca tttcattcgc agatgagtag
     1981 gcagaatcaa gatctgtttt cgataattca ccatgtaaat gaaaatgatg ccacaatcca
     2041 tcatggtgaa aaaaatcctg cttgcgaaac ttcttgagcg tacctatcat tcgtaaagcg
     2101 cgtgaaacag aaaccgggtt gaacagtacc ccaaaaggat tagctgtaat ctgaaaccct
     2161 gcatttttca ttcgaccaga aacatgagta ctaaagcagg agcctataaa gaaaaccgac
     2221 tccttatgcg tgatcttgcg gtttgcttca actggttcaa atacagtacg gaactgatct
     2281 ttgtttttac ttttcatatc aaaagcatgg gatcggggtg gatgtaattg tattgtaaat
     2341 gattcttcaa tttttcaccg gaaacaattt tccaattatc tgaaggcaaa tcaagaaatt
     2401 cgggaagagg taatcctgca ttcattgctg cccgggtata aaaatcagcg cgcaaagggt
     2461 gttgatcgca aacaccatta taaatctcac caaaagcatc tcttgaaatg atcttttcga
     2521 taatgcgtat gcaatcgtcg cgatgaatca ggttgaccgg cgattgccca tccttcaact
     2581 ctttttttcc agccaaaaaa cgcgcaggta aacgatcata accgattaat ccggcaaacc
     2641 gaattaatgt tactttgctg ccactctcgt ctcttaaaat ccgttctgca tccagtaagg
     2701 cttttccgct tgctttaaca ggaggtgctt cacagttttc gtcaaccaat gtattcaaat
     2761 ccggataaac tgatgttgag ctcacaaaca acacgcgttg aattttattt ttcctgattt
     2821 gttcagctat taattttatc tgaagaggat gttgggaagc tttttcttca tttcttgcgg
     2881 gaggaaaatt tattatcagc acatcacaga aaaaaacagc atcatccgag aaagaaagat
     2941 cctctgatgt cgctttcagg ctgcatattt ttactccgga ggaatccaac tcactatcac
     3001 ttctccccga tgtgttggta gccatcacct catatccggc atttctaaga tggatagcca
     3061 aaggttttcc taaccaacca cagcctatta ttgaaatttt tcggacagaa tttgctttca
     3121 tactccaaat tcgctttgaa tgattgcctc cgcttcaaca gatgattgaa tatcataaaa
     3181 cgctctgtca cgtccgataa tccaaaaacg tgtggctgaa ttcagtgcca aatctatttc
     3241 gtgggtagaa agcacaaatg aatgtttttg ctctttggcg agctcacgta attttgcagc
     3301 catttttcga cgcatcgaca aatcaagaaa agcggttggc tcatcaagaa caactaaagg
     3361 tgtttgttgc gccagcgcac gcgcaatcat cacccgctgg cgttctccat ccgataactc
     3421 agcaactttc cgccatgcaa aatcgctcaa atccatctgc tccatcactt ctgctattat
     3481 tctgtgatca tcgtctttta aacggccaaa caatcctgta tagggaatac gacctaacgc
     3541 aatcaaacca taaacatcta acgaacctgt atccggagta cgtgccaaaa ccattcccac
     3601 gttcctcgac cattcttctc ctgaaaaagt ccgggtatct tttccattga taaaaagttg
     3661 tccggccaaa atggacttca ttcctgcaag agaacgcaac aatgttgatt taccacttcc
     3721 atttgcacca agcaaagcaa tgatttcaga ttcgtcaata cttaaattca aatcttccag
     3781 cagcgcacag tcacccgaag catgcgcata acccacataa agtgattttg tttgaacaat
     3841 catgcgaagc ctcctttgtg acgttgtttt aaaataacga tcagaacaac cggtgcacct
     3901 acaagagaag taacagcatt tagtggaaga ggcgtttcaa aaacgggcaa acgcgtaatc
     3961 aaggaacaaa aaagtgaaag cgcagctccc aataaaacca cagcaggcaa aagcagcgaa
     4021 tgatcttcgg tgcgaataac ccatctgact aaatgtggaa ctgcaattcc gataaatgca
     4081 atcggaccac aatacgccgt aacaattgcg gtaagcatac cggatgaaat aattattccc
     4141 aatcgaaatt tcttcaatga aacacctaca gattgcgcgt acacaggtcc caacacccat
     4201 gcattgagcg aacgggtaag aaaaaatgaa ggaatgagta atacaattat taaagccgaa
     4261 aaacgaagag atgatgtcag gtccatacta ctgaagcttc caaatcccca aaaaacaaat
     4321 tgctgtaatc cttcacgtgc agcagcatgt tgcaacaaat tcaccaatgc acctgccaga
     4381 taagcgatca tcaatcctcc aattataaca gtaaccccat cacgcgaaac gcccgacaaa
     4441 aaaaccacaa gaacaagaac aagtattgcc ccagtcatgg cagaaaatac ctgaagaaat
     4501 cctgaaagac tacctgaaaa atgcatccct aaaagatcac cacccataaa aacaatcgct
     4561 accccaagca tagcaccgga cgaaatcccc aacacatccg gacctgcaag cggattacgg
     4621 aaaaatgtct gtaataacaa tccaccgcaa gccagagctg cacccgcaga aagtgcagtt
     4681 aacgactgcg gtagtcgtgt atccagaatg atataattcc aggcttcctt ttcagaagta
     4741 ccacctgtta aaactgttac tacctctgaa aatgggatag aaacagatcc caaaaaaagg
     4801 tcaagtaaaa acaaaaccgg aacagcacat gccaagacga aaatcagtaa aaaagatttt
     4861 cgtcgttgtg cagtatactg ttgtgatagt ctgttcattt gagttgttga aaatacacag
     4921 gagtatgtcc ggaaacaatt tttggctgaa agaaggaaag caggtctttc aacagcacct
     4981 gaggttcagc tacagcagcg ccgaaataat cgcatttatt ggtattgcaa aaaatgacct
     5041 ttccgttttt gaaagcatca aattcagcat acctaggatc ttcctttcgc aattgttcca
     5101 aattaaaatc atcgttacat gcacccacaa tcacccacca ttcggcgttc atggccttat
     5161 ccataactac ttccatattg caggtaagcg aattgttatg agcatcgtct ctccacaaat
     5221 aatttccacc ggcatccacc aataaacgtg ctgcaaaact tttccctccg ggcacatgcc
     5281 attctccatt ataggccttt ccggaaaaca cagtgggaga atcacctgaa tgatccgctt
     5341 gtttgataag agcgatgtat tcgttc
//
LOCUS       DAOMYG010000209         5354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5280_length_5354_cov_7.959426, whole genome shotgun sequence.
ACCESSION   DAOMYG010000209 DAOMYG010000000
VERSION     DAOMYG010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5280_length_5354_cov_7.959426"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2302)
                     /gene="pbpC"
                     /locus_tag="PK610_07810"
     CDS             complement(<1..2302)
                     /gene="pbpC"
                     /locus_tag="PK610_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HRJ35878.1"
                     /translation="MFRRTRQFISRFRWYFIAFGSWFLVWYYFALPKQLFTDPTCTVL
                     YDKKGDILGARIAEDGQWRFPHNENVPEKFAKALIFFEDKTFYRHIGISARGIGRALK
                     QNFSQGKVVSGGSTLTMQIVRLMRKGRGRSISEKIIEAVLATRIELAYTKNEILAYYA
                     SNAPMGGNVVGLDAAAWRYYGRTPDELSWAESATLAVLPNAPSHIFPGKNQSRLLEKR
                     NRVLKGLYEQHEFDTTTLITAIAEPLPSAPPDLPQMAPHLMDRLIRYGHKGKGIHTPI
                     DYQLQDHVNRILQRHHEKLQENQIFNAACIVVDVNEGNVIAYTGNVKNEDPEHGSDVD
                     IITAPRSSGSILKPLLYAGMLNDGLITPGMLFPDVPTHISGYSPKNYYQRYDGKNYYQ
                     RYDGVVPANRALSRSLNVPAVRMLQQYGIAKFHYLLKRIGFSSLVFPSSHYGLSLVLG
                     GAEVSLWDLAHVYASMARVLNQYPDFNSDLYKRPRLILEDTTAQPFSESIEPVLNPAS
                     IYFTMQAMVEVARPDEDANWQYYSGSSRIAWKTGTSFGFRDAWAVGVSPGYVVAVWVG
                     NADGEGRPGLVGIEAAAPILFDVFSRLPKTGWFKKPFDDMAKVPICRLSGHRASTLCS
                     PTDTIYIPQSSLATQACPYHQLLHLDQEGKFRVDGNCVDPSEMKNEAWFVLPPVMEYY
                     YKSRNPSYKPIPPYRSDCIAKTDEQQMEIIYPKKPSKIYIPIGIDGEPEKTVFEATHR
                     NKNAVIYWHLDEYFLGATSSIHQMELK"
     gene            complement(2393..>5354)
                     /locus_tag="PK610_07815"
     CDS             complement(2393..>5354)
                     /locus_tag="PK610_07815"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRJ35879.1"
                     /translation="VNFRNYSVADGLAQSQVFAITEDAGGYLWFGTKGGGLSRFDGLT
                     FKNYTIRDGLINDFVNCILKLRNGNLCIGTNIGISIYDGKSFNNYQVGRDSIGLPVQA
                     LAEDAEGKIWIATTKGMYLLKNDQITCFVCKHDHPREIISCIYIDNKQKVWYGDDFGL
                     NRIDSIEGKIRITPFRRKEGFTNVLVRTIREEKNGTLIIGTYGGGLHQLRGKMAVPFA
                     FNKNLESRIIHDLLIDSKGDWWFSTYDAGIYRLRTNDSSLVHYGLSEGLANDHASVLF
                     EDSWGNIWAGTSGGGVSRFSGERFKHYTERNGLPGNYVYSVCPQGDSLLWLGTSAKGV
                     VRMNLNSGIHQRFGVEQGFVEEKIKTIHTDSSGNIWLGTEGEGLWIYDSTGFKKITTK
                     NGLSGNWIKDILIAKSGDIWIATAGGGISVIQQREPLRIKKISEKNGLSRNRINALLE
                     DHQGRIWYASEGGGVGMISENGIKNFTTVNGLSGNLVRSLREDQNGFLWVATGGGGLS
                     RIDIYRDMSIDRITRDNGLSSDNLYLLEIDQQNNLWAGSENGVDRILLSENSEVREIK
                     HYGKAEGFMGIETCQNSVALTPDGSIWFGTIDGLTRHDPRNSDRNLLPPRLQFTSVNL
                     FYQPLQNTRFSAHVENWGKYRDQLELNYDENHIGFEFIGIDHRNPSSVKYQWKLEGAD
                     TSWSPLSLKHDVTYSNLLPGDYTFTVRAVNEDGIMTASPLNYHFRIKAPWWQQWWFRF
                     AMIILGMAIIAGIILLVTRRARKRVQSMREKLKVEKKLLELEQKALRLQMNPHFIFHA
                     LNSIQGLIVKKDEKTARLYLAKFSKLMRAILENSREQVIPLEKEMETLQDYLTLEQFT
                     RDHSFDFSISTEGEPDCSEIMIPSMLLQPFVENSIIHGFNGLDRRGKIEIVFRMVNNT
                     LECIVRDNGVGREKALQNRMQQDSHHKSMALIVTQERLDLLRKQSGEKTPAEPITITD
                     LAEGTEVLVRIQIS"
BASE COUNT         1376 a         1213 c         1108 g         1657 t
ORIGIN      
        1 gttttaattc catctggtga atggagctcg ttgcacccag aaaatattcg tccaggtgcc
       61 aataaataac ggcattttta ttgcggtgtg tagcctcgaa aactgttttt tccggttcgc
      121 cgtcaatgcc aatgggaata taaattttgc ttggcttttt tggataaatg atttccatct
      181 gttgctcatc cgtttttgca atgcaatcgc tgcgataagg tggaatcggt ttataggatg
      241 ggtttcgtga tttgtaataa tactccataa ccggtggcag aacaaaccag gcttcatttt
      301 tcatttcaga gggatcaaca caatttccat ccactctgaa ttttccttcc tgatcgagat
      361 gcagcaattg atgatacgga caagcttgtg ttgccaatga actttgcgga atataaatgg
      421 tgtcggttgg agaacacaag gttgaagcac ggtgtccgct taatcggcag atggggactt
      481 ttgccatatc gtcgaatggt tttttgaacc aacctgtttt tggtaatcgc gaaaagacat
      541 caaataatat aggagctgct gcttctattc caaccaagcc gggacgacct tcaccgtctg
      601 catttccgac ccatacagct acaacataac ccggactaac tcccacagcc caagcatctc
      661 tgaatccaaa actcgttcct gttttccagg cgatgcgtga agagccggaa tagtattgcc
      721 aattggcatc ttcatccgga cgcgcaactt ctaccattgc ctgcattgta aaataaatgc
      781 ttgcaggatt tagtaccggt tcgatgcttt ctgaaaatgg ttgtgcagtg gtgtcttcta
      841 aaattaaacg cggacgtttg tacaaatcgg aattaaaatc cgggtattga ttcagtactc
      901 gcgccatgga agcgtagaca tgtgccagat cccacaggct aacttctgca ccaccgagca
      961 caagtgataa tccgtagtgc gacgatggaa acaccaatga agaaaacccg attcgcttta
     1021 ataaataatg aaatttagca attccatatt gttgaagcat tcttacagca ggaacattga
     1081 gcgaacgtga caacgcacgg ttagcaggaa cgacaccatc gtaacgctgg taataatttt
     1141 taccatcgta acgctggtaa taattttttg gagaataacc cgaaatatga gtggggacat
     1201 ccggaaacaa cattcccgga gttattaatc cgtcattgag cattcctgca tacagcagcg
     1261 gttttaaaat cgatcccgag ctgcgtggtg cggtgatgat atctacatca ctaccgtgtt
     1321 cgggatcttc attttttaca tttccggtgt aggcaattac atttccttcg ttaacatcta
     1381 caacgataca agccgcattg aaaatttgat tttcctgtag tttttcgtga tggcgctgca
     1441 aaattcggtt gacatgatcc tgcaattgat aatcaatggg agtatgaatg ccttttcctt
     1501 tgtgtccata tcgtatcaga cgatccatta aatgcggagc catttgcggc aaatcgggtg
     1561 gggcagaagg caagggctct gcaatagcag tgatgagtgt tgtagtgtca aattcatgtt
     1621 gctcgtacaa tcctttcaaa acacggtttc ttttttcaag tagtcgcgac tgatttttcc
     1681 ccggaaaaat atgacttggt gcattgggca gaacagccaa ggttgcagat tctgcccatg
     1741 ataattcatc gggagtgcgt ccataatacc gccatgctgc agcgtcaagt ccaactacat
     1801 tccctcccat tggtgcattg gatgcgtagt aggccaggat ttcgtttttg gtatatgcaa
     1861 gttcaatacg ggtagcaagc actgcttcaa ttattttttc ggagatgctt cgtcctcttc
     1921 ccttgcgcat gagtcgtaca atctgcatgg tgagtgtact tccgccgctt actacttttc
     1981 cctgactgaa attctgtttc aatgctcgtc cgatccctct tgcgctaatt ccaatgtgcc
     2041 ggtaaaacgt tttgtcctca aaaaagatca atgccttggc aaatttctca ggtacatttt
     2101 cgttatgcgg aaatctccac tgaccgtctt ccgcaatgcg tgcacccagg atatctcctt
     2161 ttttgtcgta taagacagtg caggttggat cggtaaacag ttgtttgggt aaagcaaaat
     2221 aataccacac caaaaaccaa cttccgaagg caatgaaata ccatctgaag cgggagatga
     2281 attggcgtgt tcttcgaaac atgaggtaaa tataggtgct cctgggtaaa agcgaaatgg
     2341 cattgagtat ttgtgtggct taaaccatta tccgtttcca ggtaggtaaa tgtcaggaga
     2401 tctgtatgcg aacaaggact tctgttcctt ctgcaaggtc agtaatggta atcggttccg
     2461 caggtgtttt ttcaccactt tgtttgcgta acagatccaa tcgttcctgt gttactatga
     2521 gcgccatgga tttatggtga ctatcctgct gcattctgtt ctgtaaagcc ttctctcttc
     2581 caactccgtt atcgcggaca atgcattcga gggtattgtt gaccatacgg aatacgatct
     2641 cgatttttcc tcttctgtcg agtccgttga atccatgaat aattgaattc tctacaaaag
     2701 gttgaagcaa catggaaggg atcatgattt cggagcaatc tggttcgcct tcggtgctga
     2761 tgctgaaatc gaaggagtgg tctctggtaa attgttcgag cgtaagataa tcctgcaagg
     2821 tttccatttc cttttcaagc ggtatgactt gttcccttga attttcaaga atggctcgca
     2881 tcaactttga gaattttgca agataaagtc gggccgtttt ttcatctttt ttcacgatca
     2941 atccctgaat ggaattaagc gcatgaaaaa taaaatgagg attcatctga agccgcaacg
     3001 ctttttgttc gagttcgagc agctttttct ccactttgag tttttcgcgc atgctttgta
     3061 ctctttttcg ggctctgcgt gtaaccagta aaatgattcc tgcaattatt gccataccaa
     3121 gaatgatcat tgcaaaccgg aaccaccatt gctgccacca gggtgctttg atcctgaaat
     3181 gataattgag cggcgatgct gtcattatac catcctcatt caccgcccgc actgtaaaag
     3241 tgtaatctcc tggtaaaagg ttgctgtagg ttacatcatg ttttaacgaa agtggcgacc
     3301 atgaagtgtc ggctccttcc agtttccatt ggtattttac ggaggaagga ttgcgatggt
     3361 caattcctat gaattcgaat ccaatgtggt tttcatcgta attcaattca agctgatcgc
     3421 ggtattttcc ccaattttct acatgggcac taaaacgtgt gttttgtaaa ggctgataga
     3481 atagatttac agatgtgaat tgcaatcgtg gcggaagaag atttctgtcg ctgttgcggg
     3541 gatcatgtcg ggttaatcca tcaatggttc caaaccaaat gcttccatcg ggagttaaag
     3601 ccacagaatt ctgacaggtt tcaatcccca taaatccttc tgctttccca taatgtttta
     3661 tttctcttac ttctgaattt tcgcttaaca gaatccgatc cactccattt tcagaacctg
     3721 cccaaagatt gttctgctga tcaatttcaa gcaggtataa attatccgaa ctaagaccat
     3781 tatctcttgt aatacgatca atgctcatgt ctctgtaaat atcaatccgg ctaagtccgc
     3841 cgcctccggt tgcaacccac agaaatccgt tttgatcttc gcgtaaactc cttaccagat
     3901 taccggataa tccgttgact gttgtgaaat tttttatccc gttttcactg atcatgccaa
     3961 cgcctcctcc ttctgatgcg taccatattc ttccctgatg atcttcgagt aaagcattga
     4021 tcctgttgcg ggaaagtccg tttttttcgc tgatcttttt tatgcgcaga ggttctcttt
     4081 gttgaatgac acttattcct ccaccagcag ttgctatcca gatgtctccg ctttttgcaa
     4141 ttagaatgtc tttgatccag tttccgctta atccgttttt cgtagtgatc tttttgaaac
     4201 cggtgctgtc ataaatccac aatccttcgc cttctgttcc aagccaaatg ttgcctgatg
     4261 aatcggtatg aatcgttttt attttttctt ctacaaatcc ttgttccaca ccgaatcgtt
     4321 ggtgaattcc tgaatttaaa ttcatgcgta caactccctt cgcagatgtt cccagccata
     4381 acagcgaatc gccttgcgga caaacactat aaacatagtt tccgggcaaa ccattgcgct
     4441 cggtgtagtg tttaaatcgt tctcccgaaa agcgggatac ccctcctccg gatgttcctg
     4501 cccagatatt tccccagctg tcttcgaata aaacgcttgc atgatcgttt gccagtcctt
     4561 cgctgagtcc ataatgaacc agtgatgaat cattcgtacg aagacgataa attcccgcat
     4621 cgtaagtact gaaccaccag tcgcctttac tgtcgatgag cagatcgtga ataattctgc
     4681 tctccagatt tttattgaat gcgaaaggaa cagccatttt tccgcgaagc tgatgcaatc
     4741 ctcctccgta tgttccaatg atcagcgttc cgtttttttc ttcgcggatg gttcttacca
     4801 atacattcgt aaatccttcc tttcttcgga atggggtaat gcggatcttg ccttctatac
     4861 tgtcaatccg gtttagacca aaatcgtcgc cgtaccatac tttttgcttg ttgtcgatgt
     4921 aaatgcagga aatgatttct cgcggatgat cgtgtttgca aacaaaacag gtgatctgat
     4981 catttttgag cagatacatt ccttttgttg ttgcgatcca tatttttcct tccgcatctt
     5041 ctgccaaggc ctgaacgggc agaccaattg aatctcgccc aacctggtag ttgttgaatg
     5101 attttccatc gtaaatagaa atgccaatat tcgtaccgat gcacaaattt ccgttgcgca
     5161 gtttcagaat gcaattcaca aaatcattaa tgagaccatc gcgtatggtg tagttcttaa
     5221 acgtaagtcc gtcaaaccgc gataatcctc ctcccttggt tccaaaccac aaataaccgc
     5281 ctgcatcttc tgtaatggca aaaacctgcg attgggcaag tccgtctgcc acagaataat
     5341 ttctgaagtt aacc
//
LOCUS       DAOMYG010000210         5352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5286_length_5352_cov_9.603927, whole genome shotgun sequence.
ACCESSION   DAOMYG010000210 DAOMYG010000000
VERSION     DAOMYG010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5286_length_5352_cov_9.603927"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..882)
                     /locus_tag="PK610_07820"
     CDS             complement(115..882)
                     /locus_tag="PK610_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35880.1"
                     /translation="MKTSFKQATIIGLAALTSLSACRKDEDPPAQTPTPTPTSIKLEL
                     EHKVGSSALVLNSNLTDAFGTVFTISRADFYVNGFAFTNDMGDTILNHTPHYVVRPNV
                     TEIVLGNVSGTHAHEMSFNIGVDPASNLSVDPATQPAGSALGVQTPSMYWSWSSGYRF
                     VVIEGNADRNADGVPESMYQFHIGMNSLYKAIELHLHQDLTANASNTVHIEIDYLRFF
                     DGINMSLSTSHTHTMDNMPLATQFMNNADSVFSVHVH"
     gene            complement(927..1859)
                     /locus_tag="PK610_07825"
     CDS             complement(927..1859)
                     /locus_tag="PK610_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35881.1"
                     /translation="MKRILFFLFLLMYSVCAHACDICNVYSGITPSDFRNSFGIMYRS
                     RYLKGNFPAQGLPEVRHAAHQTDTGITKGKELFNVAELRFRLFGGKKKQWNHLFILPL
                     VNNYRSLNGYTQFDVYGLGDPTIISNRILFNTQSDSAEFTHRLSIGAGVKLPFGGRKF
                     HYLGKTADLDMQGSSGSTDFLLTMEYIGKKGPSGWIVFSSYKINTSGTESYKYGNSFN
                     LNLLMFYQAKLSPGKMLMPNWGFLLEKAAADRQSGATVDASGGRVGMLHLGLDYFSGR
                     MQWQFSYQPGLFSVMNGQQFPLINRFILGMTVSL"
     gene            complement(1913..2296)
                     /locus_tag="PK610_07830"
     CDS             complement(1913..2296)
                     /locus_tag="PK610_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35882.1"
                     /translation="MLRILTVFVLLTTFLTGSGIRTFITVRFHFQQEEIAKTRCENKA
                     KPELKCNGKCQLAKELKQAEEKSSDPAQPVNPSSYSFPDLHVFPLANTDFNLNLFPTK
                     VKRFGSLFYHPLSGTSELSAPPPEC"
     gene            complement(2297..3958)
                     /locus_tag="PK610_07835"
     CDS             complement(2297..3958)
                     /locus_tag="PK610_07835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HRJ35883.1"
                     /translation="MKKLLFSALLVVFAFTVKSQENIVPGQVMVMLHHQESPSAFVER
                     LNALYPGIAFRVEQVLSSDINIHLLRFNDLQYSHTEALNAVVRDKATALAQFNHMNVT
                     LRDTCPNDPSFGNQWAFYNNGSNGGSGTSDIEACNAWSVGRGGLTAQNDRIVVAVIDG
                     GFDLNHPDISYYVNPGEIPGNSIDDDNNGYVDDVNGWNASNNNANVQGGSWESHATHV
                     SGTVGAKGGNSTGVTGVNWDVDILPIKGSSGNESIVVAAYSYVLKMRRTYNTTNGAEG
                     AFVVSTNSSFGVDLGNPANYPIWCAMYDTLGKEGILSATATANANYNVDTQGDIPTAC
                     PSNWMISVTNTRSDDSKATAGYGATTIDLGAPGTNIYSTTMNGNYGNMSGTSMATPHV
                     AGTIALMWSVACTQLITDYKNDPDALALQMKSYLLNDGVDSVFALQNITVTGGRLNLY
                     KALLAVQEYDCTSGSIGIEETAATLLRFYPNPATDEITLIVPEGMEGLINISIFNMTG
                     QQVLSKSFSGQNMISLSLLDLCAGIYSISLSTSNNAAIFGTLIRQ"
     gene            4024..>5352
                     /locus_tag="PK610_07840"
     CDS             4024..>5352
                     /locus_tag="PK610_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRJ35884.1"
                     /translation="MSIQTSGNAPLSKGQGKNFLFYNASAGSGKTFTLVREYLRLALS
                     GDNPNYFRKILAITFTRKAAAEMKERVLHHLRVLSADPLSPLYDPLLLDEYRKVLSLP
                     NEMIRMRSERTLKAILHNYSELSVSTIDSFVHLIIRTFSRDLQLPMDFDVVMEKETIV
                     DEAVDRLIDDAGKDERITAMLNSWLQQNTSEGDRWDVANLMKEFANKVLFREDSVEPL
                     AKLSEIPAEELNTLILKLKDQVNTFRAAQVKVAEEIIDRITKMGLSVDDFSYKQTSAF
                     AAFLKVKAIGGRLEELKFGTRFTEVVEKDNWFSTDFKKKNGALVDEVRPFLNDAQKKI
                     CAALKDATYFAAMHLYREIHQVLLLHEIRSRIETIKEERDLLFIDDFNRLVSALVKYD
                     PAPFIYERVGERYDHIMIDEFQDTSVMQWHNFIPLVENTLAMGKTSLIVGD"
BASE COUNT         1505 a         1108 c         1208 g         1531 t
ORIGIN      
        1 aaggtgcctt ttgttgaagg cggatcttcg ggtgttcttt tgcaagaaac gagtcctgca
       61 atagcgagca gcgcgatggt tatcctgaat ttcatatttc tttagattaa atgattaatg
      121 cacgtgaaca ctgaatacag agtctgcgtt attcatgaat tgcgtagcca aaggcatatt
      181 gtccatggtg tgagtgtgcg atgtgcttaa ggacatgttg attccgtcaa agaagcgaag
      241 ataatcgatt tcaatgtgaa ccgtgttact tgcatttgca gttagatcct gatgcaggtg
      301 caattcaatg gccttgtaca atgagttcat gccaatatga aactggtaca ttgattccgg
      361 aacaccgtca gcgttacgat cagcatttcc ttcgatcacc acaaaacggt aacctgaact
      421 ccagctccag tacatcgacg gtgtttgtac acccaaagca gatcctgcag gttgtgtagc
      481 ggggtcaacg gaaagatttg atgcgggatc aactccaata ttaaaactca tttcatgtgc
      541 atgtgtgcct gatacattgc ccaaaacaat ttctgtaacg tttggacgaa caacataatg
      601 aggcgtgtga ttgagaatgg tatcacccat atcgttggta aatgcaaatc cgttcacata
      661 aaaatctgct cttgaaatgg taaacacagt accaaaagca tcggttaaat tggaattcaa
      721 taccaatgca gagctgccaa ctttatgttc cagttccagt ttaatggatg ttggtgtagg
      781 ggttggcgtt tgcgccggtg gatcttcgtc tttacgacat gccgaaagtg aagttagagc
      841 cgcaaggccg ataattgttg cctgtttaaa tgaagttttc atacttgtaa gttagtttaa
      901 tttcagttca ttttttgtga tatcccttac agacttacag tcatgccaag aataaaacgg
      961 tttataaggg gaaattgttg cccgttcatt acactgaata gcccgggttg gtagctgaat
     1021 tgccactgca tccttccact gaaataatcc agtccgaggt gcaacatccc aactctgcct
     1081 ccgctggcgt caactgtagc gccactctgg cgatctgcag cagctttttc cagcagaaat
     1141 ccccagttgg gcatcagcat ttttccgggt gatagtttag cctggtaaaa catcagcaga
     1201 ttcaggttaa atgaattacc gtacttgtaa ctttctgttc ctgatgtgtt gattttatag
     1261 ctggagaata ctatccaacc gcttggacct tttttcccaa tgtattccat tgttaagaga
     1321 aaatcggtgc ttccggatga cccttgcata tccagatcag cggttttccc cagatagtgg
     1381 aatttacgtc cgccaaatgg tagtttaacg cctgctccaa tgctcagtcg gtgtgtaaat
     1441 tctgctgaat ccgattgcgt attaaaaaga atcctgttgc tgataatggt cggatctccg
     1501 aggccatata catcaaactg ggtgtatcca tttaaactcc ggtaattgtt taccagagga
     1561 agaatgaaca aatgattcca ttgtttcttc tttcctccga ataaacgaaa gcgtaattca
     1621 gcaacattga acagttcctt tcccttggtg attccggtat cagtctggtg tgcggcatga
     1681 cggacttcgg gtaatccctg agcaggaaaa tttcctttca ggtaccgact tctgtacatg
     1741 atgccaaaac tgtttctgaa gtcgcttggt gtaatgcctg aataaacatt acagatatca
     1801 caggcatgcg cgcatacgga atacatcagc aggaaaagga aaaatagtat ccttttcatg
     1861 gtttgatttt ttgagtagta tcagcttttg ctgacgaaac agtgaaagag gatcagcact
     1921 caggtggagg cgcagagagt tcagaagttc ccgatagcgg gtgatagaaa agactgccga
     1981 agcgtttaac tttcgtgggg aataaattca gattgaaatc tgtatttgcc aaaggaaata
     2041 catgtaaatc gggaaaggaa tagctgcttg ggtttacagg ttgcgcagga tctgaacttt
     2101 tttcttctgc ctgtttgagc tctttggcaa gctgacactt accattacat tttaactccg
     2161 gtttcgcttt gttttcgcaa cgtgtttttg caatttcttc ctgctgaaaa tggaaacgaa
     2221 ctgtaatgaa tgtcctgatc cccgaacctg taagaaaggt ggtaagcaga acaaatacag
     2281 tcagaattct caacatttat tggcgaatta atgtgccaaa tatagcagca ttatttgaag
     2341 tgctcaaact gatagaataa atacctgcgc acaaatccag caaactgagg gagatcatgt
     2401 tttgaccact gaaacttttg ctcaaaacct gttggccggt catgttgaat atgctgatgt
     2461 tgattaatcc ttccattccc tccggtacga tgagcgtaat ttcatcggtt gccgggttgg
     2521 ggtaaaatct caatagtgtt gcagctgttt cttcaatgcc gattgatccg cttgtgcaat
     2581 cgtattcctg cactgccaat aaggctttgt atagattcag tcttccaccg gttacagtga
     2641 tgttttgcaa agcgaacaca gaatcgactc catcattgag taaataggac ttcatttgta
     2701 aagccaatgc atccggatcg tttttgtagt cggttattaa ttgtgtacag gcaaccgacc
     2761 acatcaatgc aattgttccg gcaacatgcg gggtagccat tgaggtcccg ctcatattgc
     2821 cataattgcc gttcatggtt gtggaataaa tatttgttcc tggtgcgcca agatcaatgg
     2881 tggttgcgcc ataacctgca gtagcttttg aatcgtcgct gcgggtattg gtaaccgaga
     2941 tcatccaatt gctggggcat gccgttggaa tatctccctg ggtatcgaca ttgtaattgg
     3001 catttgcagt agctgttgcc gatagaattc cttctttacc caatgtgtcg tacatagcac
     3061 accatatagg gtaattggca gggtttccaa gatcaacgcc aaacgaggag ttggtggaca
     3121 caacgaaagc gccttctgca ccgttggtgg tgttgtaggt gcgacgcatt ttaagaacat
     3181 acgaataggc agcaacaaca atagattcgt ttccgcttga acctttgatg ggaagaatat
     3241 caacatccca gtttacaccg gtaactcctg tcgaatttcc acctttggcg ccaactgttc
     3301 cggatacgtg ggtggcatgc gactcccagc ttccgccctg tacatttgcg ttgttattgc
     3361 tggcattcca tccatttacg tcatccacat aaccgttgtt gtcatcatca atgctgtttc
     3421 ccggaatttc gccgggattt acatagtaac tgatatccgg atgattcagg tcaaagccac
     3481 catcaatgac tgcaacaacg atccggtcat tttgcgcagt taatcctcct cttccaaccg
     3541 accatgcatt gcaggcttca atatcggaag taccgctgcc accgttggat ccgttattgt
     3601 aaaaggccca ctgattgcca aaagaagggt cgttcggaca ggtatctctg agagttacat
     3661 tcatatgatt gaattgtgca agagctgtcg ctttatctcg aacaactgca ttgagtgctt
     3721 cggtatgact gtattgcaga tcattgaaac gaagcaggtg aatattgatg tcagaagaaa
     3781 gcacttgctc aacacgaaaa gcaattccgg gataaagagc attaagtcgc tcaacaaatg
     3841 ctgacggaga ttcctggtga tgcagcatca ccatcacctg acccgggaca atattttcct
     3901 gtgattttac agtgaatgca aaaaccacca gtaaagcaga gaataatagc tttttcatta
     3961 atgaaggatt attccgaaag atcattaatt ttagggagaa aagcaagcga attgtagcaa
     4021 cggatgagta ttcaaacaag cggaaatgcg cccctatcaa aagggcaggg aaagaacttc
     4081 ctgttttata atgcttcagc aggatcaggc aagaccttta cccttgtgag ggagtatttg
     4141 cgccttgcac tctcgggaga taatcccaat tacttccgta agatccttgc catcactttt
     4201 acccgaaagg ctgcagcaga gatgaaagaa cgcgtgctgc atcatttacg tgtattgtcg
     4261 gccgatccgc tctccccgct ttatgacccg cttttattgg atgaataccg gaaagtttta
     4321 agccttccca acgaaatgat tcgtatgagg agcgaacgaa cgctaaaggc tatccttcac
     4381 aattattcag aactttctgt gtcaaccatc gatagttttg tccacctcat catccgaacc
     4441 ttttcccgcg atctccaact gccaatggat ttcgatgtgg ttatggaaaa agaaacaatt
     4501 gttgatgagg ctgtcgaccg attgattgac gatgcaggaa aagatgagcg tatcacagca
     4561 atgctaaaca gctggttgca acagaatacc agcgaaggcg atcgatggga tgtggcaaac
     4621 ctgatgaaag aattcgccaa taaagtactc tttcgtgaag acagtgttga accattagca
     4681 aagctttcag aaataccggc agaagaattg aatacactca ttttgaaact taaagatcaa
     4741 gtcaacacgt ttagggctgc gcaggtaaaa gtcgcggagg aaattattga ccgaatcacc
     4801 aagatgggtt taagtgtcga tgatttctcc tacaaacaaa cgagtgcttt tgcagcattt
     4861 ttaaaagtga aggcaattgg tggtcggctg gaagaattaa aattcggaac acgatttacc
     4921 gaggtagtgg aaaaggacaa ttggttttcc actgatttca aaaagaagaa tggcgcattg
     4981 gtagatgaag tcagaccttt tttgaatgat gcccagaaaa aaatttgtgc tgcattaaaa
     5041 gatgctactt atttcgcagc aatgcattta tacagggaga tccatcaggt gctcttgttg
     5101 catgagatcc gatcacgcat agaaacaata aaagaagaac gtgatctttt gtttattgat
     5161 gatttcaaca gattggtttc tgcattggtg aaatatgatc ccgctccgtt catctacgaa
     5221 agagtaggcg aacggtacga ccatatcatg attgatgagt ttcaggatac ttcggtaatg
     5281 caatggcata attttattcc tttggtggag aacacgcttg ctatgggaaa gaccagttta
     5341 attgtaggtg at
//
LOCUS       DAOMYG010000211         5340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5303_length_5340_cov_9.076254, whole genome shotgun sequence.
ACCESSION   DAOMYG010000211 DAOMYG010000000
VERSION     DAOMYG010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5303_length_5340_cov_9.076254"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PK610_07845"
     CDS             <1..341
                     /locus_tag="PK610_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35885.1"
                     /translation="ALELKKQVVRIVGDQRLSTYEFNGERAAFFQSMSWYYLAASFSN
                     VLAAIGAFFMRAYKREGFFIYLFSHILFFVAPFILVLRYEPRWGETAVLFVFAILFIA
                     LYARQLSHFK"
     gene            338..832
                     /locus_tag="PK610_07850"
     CDS             338..832
                     /locus_tag="PK610_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35886.1"
                     /translation="MKANTYDIKFTRITRFLLGDQSPRLFTQLVFYISLPVVFWYLMF
                     FSFTLVAMRMVDALPNANQVHEGIVRLGDMYSVSDMQGAFLRMSVIGLVSTGLMIAGI
                     LFLWRRWKIAYSLILPGIVGCLFCPWIFFGSEYFSREQSSWEWALPSTLCILVIIDWA
                     TRGK"
     gene            complement(862..1239)
                     /locus_tag="PK610_07855"
     CDS             complement(862..1239)
                     /locus_tag="PK610_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRJ35887.1"
                     /translation="MKTIVHTKEAPLPIGPYSQAVLINHTLYVSGQIAIDPATGNMMM
                     NTIEEETHQVMKNLSAVLKAVEMDFSHVVKASIFLSNMDDFPKVNTVYGSYFKDHYPA
                     RETVQVSRLPKDVNVEISVIAVK"
     gene            complement(1439..1648)
                     /locus_tag="PK610_07860"
     CDS             complement(1439..1648)
                     /locus_tag="PK610_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRJ35888.1"
                     /translation="MKSNVGTADRIIRILISAVLAGLFFSGKVTGTVGIIMVVAAGIF
                     TITAAIGFCPLYKLVGLSTCPVKKS"
     gene            complement(1738..2160)
                     /locus_tag="PK610_07865"
     CDS             complement(1738..2160)
                     /locus_tag="PK610_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35889.1"
                     /translation="MRFALFMAIFCLFLSACKKDPGERSMDRNMRLLCREKWVVKSVS
                     DSFVTTIPPFSLLEFEFKSEGKLVVSEPGLFVHGSFTLIQSTNKVLSIKANTVTSLSN
                     AIIYFNRIWTIQTLDRRKMIWGISGLDYIRAEFVHPDE"
     gene            complement(2210..2803)
                     /locus_tag="PK610_07870"
     CDS             complement(2210..2803)
                     /locus_tag="PK610_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase hexapeptide repeat family protein"
                     /protein_id="HRJ35890.1"
                     /translation="MIYEFNGFRPVVHESSFVHPQACVTGNVIIGKNVYIGPGAAIRG
                     DWGMIIIKDGCNVQENCTIHMFPGVTVTLHEGAHIGHGAIIHGASIGKNCLIGMNAVL
                     MDNVELDDECIVGALTFITAESKFPKRQLIVGNPGKAIKEVSDEMIDWKTKGTALYQQ
                     LPSELYAGLKPCEPLREIEPDRPQQEAMYKSWKEINK"
     gene            complement(2860..3297)
                     /locus_tag="PK610_07875"
     CDS             complement(2860..3297)
                     /locus_tag="PK610_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRJ35891.1"
                     /translation="MSMLKTFEDLDVYKKCREFRQDISQLVKTLPNTENHKLQSQLLR
                     SSRAVTSSIAEGFGRFHHLENIQFCRQSRGSLMETLEHLIIANDEGFISAETLEDFRM
                     KQNDCMRTLNAYISYLKKAKNRAFESAPATPYVQDQQFQEQQY"
     gene            complement(3356..4561)
                     /gene="pcaF"
                     /locus_tag="PK610_07880"
     CDS             complement(3356..4561)
                     /gene="pcaF"
                     /locus_tag="PK610_07880"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015362810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HRJ35892.1"
                     /translation="MKDVFISTAVRTPIGNFGGTLAAVRTDDLAAHVLRALMEKVPTL
                     DPAAIEDIVMGCANQAGEDNRNVARMASLLAGIPFSVPGETVNRLCASGMSAILNASR
                     GLQSGNGDIAIAGGVENMTRGPWVISKASGAFGRDQQMFDSSFGWRFINPKMQELYGT
                     DGMGETAENLVDLYKIEREAQDAFALWSQQKAAKATANGIHAQEIAAVTIPQKKGDPL
                     VFAADEFIKASTTMETLGKLRPAFRKDGSVTAGNASGLNDGAAAMLLATETGLKQHGL
                     QPMARIVSSAVAGVEPRIMGIGPVLASEKALKRAGLTLDQIDVLELNEAFAAQVLSCT
                     RKMGLADNDPRININGGAIALGHPLGMSGTRLVQTAALELQRSGKKYALCTMCIGVGQ
                     GYAIIIERI"
     gene            complement(4587..5012)
                     /locus_tag="PK610_07885"
     CDS             complement(4587..5012)
                     /locus_tag="PK610_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HRJ35893.1"
                     /translation="MDPIRLAEKVVDSMYNNDPFSKWLGIERIEDGAGISVLRMTVNK
                     EMLNGFGIAHGGITYSLADSALAFASNSHGKKCVSIETAISHLTAVKEGDILTARAVE
                     KNCSNKIALYEVDVTNQNGKLVASFRGTVYRTSQEWDVK"
     gene            5155..>5340
                     /locus_tag="PK610_07890"
     CDS             5155..>5340
                     /locus_tag="PK610_07890"
                     /inference="COORDINATES: protein motif:HMM:NF020922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 family protein"
                     /protein_id="HRJ35894.1"
                     /translation="MSVYFTPAFNKFFIDLAPNNNKDWFDVNRNRYEQDVKLPFRGFT
                     ADVIKEISKIDPTVKMEA"
BASE COUNT         1458 a         1137 c         1101 g         1644 t
ORIGIN      
        1 aggcattgga attaaaaaag caggttgttc gaattgttgg tgatcaacgt ttaagtacct
       61 atgaattcaa tggcgagcgc gcagcatttt ttcaatcgat gagttggtat tatctggcgg
      121 cttctttttc gaatgtgctt gcagcaattg gcgcattttt catgcgtgcg tataaacgcg
      181 aaggattttt catttatctc ttctcccaca tcttgttttt tgttgctcct tttattttgg
      241 ttttgcgtta tgaaccgcgt tggggagaaa ctgctgttct ctttgttttt gcaatcttgt
      301 ttatcgcatt gtatgcgcgt cagctatctc attttaaatg aaagcgaata cctacgatat
      361 caagtttacc cgaatcaccc gttttttact cggtgatcaa tccccgcgat tgtttacgca
      421 attggttttt tacatcagtt tacctgtggt tttctggtac ctgatgtttt tttcatttac
      481 cctggtggca atgcgtatgg tcgatgcgct acccaacgca aatcaggttc atgaaggaat
      541 tgttcgtttg ggtgacatgt attctgtaag tgatatgcaa ggtgcatttt tacggatgtc
      601 ggtaattgga ttggtatcga caggattaat gatcgccggt attctttttt tatggagaag
      661 atggaaaatt gcttactccc ttatcttgcc cggaatagtg gggtgcctgt tttgtccctg
      721 gatctttttt ggaagcgaat atttcagccg cgaacaaagc agttgggagt gggccttgcc
      781 ctctaccttg tgtattctgg taatcatcga ttgggcaacc cggggaaagt aattcttctt
      841 ttacccggtt gaatgcagtg attattttac agcaataaca gagatttcca catttacatc
      901 ctttggtaat cgggatacct gtaccgtttc acgtgcggga taatgatctt tgaaatagga
      961 accataaacc gtattcactt tcgggaaatc atccatgttg gaaagaaaaa tactggcttt
     1021 aaccacatgc gaaaaatcca tttcaaccgc ttttaaaaca gctgagagat ttttcatgac
     1081 ctggtgcgtt tcttcttcta tcgtattcat catcatattt cctgtggcgg gatcgatagc
     1141 gatctgacca ctgacataca acgtgtgatt gataagtaca gcctgactat atggaccgat
     1201 aggaagtggg gcttctttgg tgtgaacaat cgttttcatt ggcgatttgc atgaaatgaa
     1261 taaaaatacg gcgacagcaa tcactgagca attattgaac aatcgaatcc tgcttgccat
     1321 accgtaaaac taccgattcc aaattagatt tggcagtttc aagtaacaga atttcaatca
     1381 ccgaaaacga tctccggtga ttgaaataat aaaatgctaa atcgtgtctt agtttggctt
     1441 aactcttctt tacaggacaa gtagaaagcc ccaccagttt gtagagcgga caaaatccaa
     1501 ttgcggctgt tatggtgaag attcctgcag caacaaccat tataattcct actgttcctg
     1561 tcactttgcc agagaagaat aatccggcaa gtacggccga aataaggata cggatgatgc
     1621 gatctgcagt tccaacatta cttttcatag tcgttttttt tatcaaagta aatctacaat
     1681 agttgtatat acagtgataa gtatcacatt caaaaaatga cattcgtcag gaatgaatta
     1741 ttcatccggg tgaacaaact cagcacggat ataatcaagc ccactaatcc cccagatcat
     1801 tttcctgcgg tcgagcgttt ggatggtcca gatgcgatta aaataaatga ttgcattcga
     1861 caatgaagta acggtattcg cttttatgct gagcactttg ttggtggatt gaattagcgt
     1921 aaaactgcca tgaacaaaca aaccgggttc gctcactacc agttttcctt cggatttaaa
     1981 ctcaaattca agaagagaaa agggagggat ggtggtaacg aatgaatcgg aaactgattt
     2041 taccacccat ttttcacggc ataatagccg catatttctg tccatactcc gttctcccgg
     2101 atccttttta caggcggaaa gaaaaaggca aaaaatcgcc atgaaaagtg caaatcgcat
     2161 tcctcaagtt aagggaaata tctcacttcg ccggaaattt ccgggtgaat tatttattga
     2221 tctctttcca tgatttgtac atggcttcct gctgcggacg atcaggttcg atttcacgca
     2281 atggttcaca gggttttagg cctgcgtata attccgaagg aagttgttgg tacaatgccg
     2341 ttcctttggt tttccagtca atcatttcat cactcacttc cttaatcgcc ttaccgggat
     2401 taccaacaat gagctgacgt tttgggaatt tactttctgc cgtgataaag gtcagcgcac
     2461 ctacaataca ttcatcgtcg agttcaacat tatccattag tacagcattc ataccaatca
     2521 gacaattttt accgatgctg gccccatgga tgattgcacc atgaccaata tgcgcgcctt
     2581 catgcaatgt aacggttacg cccggaaaca tatgaatggt acagttctcc tgtacgttgc
     2641 atccgtcctt gataatgatc attccccaat cgccacgaat ggcagcgccg ggaccgatat
     2701 atacgttttt tccaataatt acattacctg taacgcaagc ctgtggatgg acaaaagaac
     2761 tttcgtgaac tacaggacga aaaccattga attcgtatat cataattttg aaattaaaag
     2821 aacgaccact gagttaccgg aatcgatcgt tctattggat caatattgtt gttcctggaa
     2881 ctgttggtcc tgaacatatg gcgtagccgg agcactttca aatgcacggt ttttcgcttt
     2941 tttcaggtaa gaaatatagg cattcaatgt gcgcatgcaa tcgttttgtt tcatgcggaa
     3001 atcttcaagt gtttctgctg aaataaatcc ttcgtcattt gcaatgatca aatgttccaa
     3061 tgtttccatc aatgatccgc gcgactgacg gcagaattga atgttttcca aatgatggaa
     3121 acgtccgaac ccctctgcaa ttgagcttgt aactgcacgt gatgagcgca gcaactgact
     3181 ctgtaattta tggttttctg tgttgggtaa tgttttaaca agttgagaaa tatcctgcct
     3241 gaactctctg cacttcttat acacgtcaag gtcttcaaat gtcttaagca tactcatggt
     3301 aataaatttt taataggtga tgtatgaatt ataaaattga ccggtaactc agtggtcaga
     3361 ttctttcaat gataatggca tagccttgtc ctacgcctat gcacattgta cacaaggcat
     3421 atttttttcc ggaacgttgc aattcaagag ctgcagtctg caccaaccga gtaccactca
     3481 ttccaagagg atgtcctaat gcaattgctc caccgttgat gttgattcgc ggatcattat
     3541 ctgccagtcc cattttacgt gtacaggata aaacctgtgc agcgaatgct tcattcagtt
     3601 caagaacgtc gatttgatcg agagtgagtc ctgcccgttt taatgctttc tctgaagcga
     3661 gaaccggacc aatacccatt atacgaggtt caactcctgc aaccgcggat gaaactattc
     3721 ttgccatcgg ttgaagtcca tgttgtttta aacccgtttc agtagcaagt aacatagctg
     3781 cagcaccatc gttgagtccg gatgcatttc ctgctgtaac actgccatcc ttgcgaaatg
     3841 cgggacgaag tttcccgagt gtttccattg tggtggatgc tttgatgaat tcatctgcgg
     3901 caaatacaag gggatcacct tttttctgtg gtattgttac tgcagcaatt tcctgtgcat
     3961 gaattccgtt tgctgttgct tttgcagctt tttgttgcga ccataatgca aatgcatcct
     4021 gcgcttcgcg ttcaatttta taaagatcaa ccagattttc tgcagtttct cccataccat
     4081 cagtaccata gagttcctgc attttcggat tgatgaaacg ccatccgaaa ctggaatcaa
     4141 acatttgctg atcgcgtcca aacgcaccgg aagcttttga gataacccat ggtccgcggg
     4201 tcatattttc tacaccaccc gcaatggcga tatcaccgtt tccgctttgc aaaccgcgtg
     4261 atgcattaag gattgcagac attccggaag cgcaaagtcg gttgactgtt tctccgggaa
     4321 cgctgaatgg gattcctgca agcaaggatg ccatacgggc aacgtttcgg ttgtcttcac
     4381 cggcctgatt ggcgcagccc atcacaatat cttcaatggc ggcaggatca agtgtcggaa
     4441 ctttttccat cagagcacgc aatacgtgag ctgcaagatc atctgtgcga acggcggcaa
     4501 gtgtgcctcc aaaattgcca atcggtgtgc gaacggcggt tgagataaat acgtctttca
     4561 tcaacgaatc agataacaac aataaatcat tttacatccc attcctgcga ggtgcgatag
     4621 accgtgccgc ggaaactggc cacaagttta ccattttggt tggtaacatc aacttcgtaa
     4681 agggctattt tgttcgaaca atttttttca acagctcgtg ctgtaagtat atctccttct
     4741 ttaacagccg ttaaatggga aattgcagtt tcaatactga cacacttttt tccgtgcgaa
     4801 ttgctcgcaa atgcaagcgc gctatctgcc agcgaataag taattcctcc atgcgcaatc
     4861 ccaaatccgt taagcatttc cttgtttact gtcatcctta acaccgatat cccggctccg
     4921 tcttcaatac gttctatacc caaccatttg ctgaatggat cgttattgta catactatca
     4981 actacttttt cggctaagcg aataggatcc ataaataagt tttcgttaaa aatacaaatt
     5041 gaagtgatag tggagattca atcggagcat ctaaattttt aattcaattc ataaaaattt
     5101 gaaattaggc ttgcgagaga ctgaaaaaaa agtaatttgc ctaaaagaaa tcttatgtcg
     5161 gtctatttca ctcctgcatt caataaattt tttatcgacc tggctcccaa taacaataag
     5221 gattggtttg atgttaaccg caacaggtat gagcaagatg taaaacttcc ttttcgggga
     5281 tttactgctg atgtgataaa ggagatttca aagattgatc caacagtgaa aatggaagct
//
LOCUS       DAOMYG010000212         5326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5329_length_5326_cov_10.465377, whole genome shotgun sequence.
ACCESSION   DAOMYG010000212 DAOMYG010000000
VERSION     DAOMYG010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5329_length_5326_cov_10.465377"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..813
                     /locus_tag="PK610_07895"
     CDS             148..813
                     /locus_tag="PK610_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35895.1"
                     /translation="MNRFYVRSFTVATALFLFGNAVAGPVANTVTSAGSAVIAQVARF
                     NVVNLGPQVEISWVGIKEKDASFYTVERSSDGVNFIEITRVSAASESPALLEYFEADR
                     DPLPGTSYYRLKQTLKSGEYLYSGQITVRRQDCGSELMGMNAGTEMDPVMFLASAAFG
                     NEEVLVVVRDREGKEFFSKIMLTFLDGKIVGFDPDGRIVDGDYIIIASSNDLLQQKKI
                     KMD"
     gene            963..1889
                     /locus_tag="PK610_07900"
     CDS             963..1889
                     /locus_tag="PK610_07900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ35896.1"
                     /translation="MISVRGLTKRYGEFAAVDHLNLRVEKGEFYSLLGPNGAGKTTTI
                     NMLNTLLDPSEGSVSINGLSLNADRNSIRRQIGVVPQEISLYDELSAFDNLRFFGSLY
                     GITESLLRVRIHHLLEEFGLIDRMNEKIKHYSGGMKRRINIASALLHQPALVFMDEPT
                     VGIDPQSRNNIYEAIQRLRKDGVTILYTTHYMEEAERFSDRIGIIDHGTIIAEGTLAQ
                     LYTRAGHGDSMVIELAEINEKQFIQIQNLNGVVVSRTENRIQFHGDDLRKKIPSIMLM
                     ITELGLDLIHLDIRKPDLEAVFLSLTGKNLRD"
     gene            1889..3103
                     /locus_tag="PK610_07905"
     CDS             1889..3103
                     /locus_tag="PK610_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ35897.1"
                     /translation="MFKIARKDLMLFFNDKKAVLLTFLMPMGLITLFVFAFGGNQKKR
                     DMNEMRLLVADEDQSKDSKELVAQLDALPTLRIEQTDWEKAKQHVSSGERSSALRIFP
                     SYSDSLQLGVSGFGFYFDQARQTEAAMIQGVLSGFLFAQEGDKVMRGSLGTQLETEFG
                     MEDSSSREMVLMMVEQMIAKNSMQDPSVSMMRLEEVRSDEGVNPTLVHAVGGTAVMTL
                     LFSVAAMGAGLLEEKEKGTLRKLLLAPIHPIQILFGKLISAVAIGFIQLWLMLIFAWL
                     VFGLQILPHIPSLFLIIFFTALCCGSFGVFLASFCTTRKQVEGLSTIIVLVMSAIGGS
                     MIPTFFMPEFMQQLSVLSVNYWSIQAFYDVFWRDAEWMVLFFKLMMLFSISLVMIAIA
                     IPLYKRNILRLS"
     gene            3141..4016
                     /locus_tag="PK610_07910"
     CDS             3141..4016
                     /locus_tag="PK610_07910"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRJ35898.1"
                     /translation="MKKYSIRDIEKLTGIKAHTIRMWEKRYNILEPDRTSTNIRKYSD
                     QELKRILNISILNSCGLKISRIAGLTDNDLAVKVLELQKENVSEDVFIERMVVAMIEL
                     NEELFEEVLDEAIEKKGFEQTMLLIVYPFLQKIGILWQTGSINPAQEHFISHLIRQKL
                     MVATDALKGKKADKETFILFLPVHEMHEIGLLFYNYLLRARGYQVVYLGQAVPFDDLE
                     KVAKIRPAKYILTFFVSPVPEDGLQEYVTKLHHTFPKTKIFVTGFQLGLEQDLSYPDN
                     IVPVKAVSEFLTHLK"
     gene            complement(4064..5284)
                     /locus_tag="PK610_07915"
     CDS             complement(4064..5284)
                     /locus_tag="PK610_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35899.1"
                     /translation="MKQKFLLFALPLLWLSASAQNILDNKVTFKYTQLPLVKITDNSR
                     TYALDVQMSYKQRNDDSLKAWEQARQNYLVTLEGYMNAYEMQTAQIDRQHLNNMVQWE
                     KQVAAGNTAAQPPVKPPYPGMNPPLRPKKPFILQDIFPASITAAIRPEGITLNAGSQT
                     RITLTFDGFEKGPVRLDKTGQAPTEKYSYVIQYRHPVTMRIDVPGRGTVFNQRVVETE
                     PFQSTRTKEFATKAELELWWFDNELPTWEERQRSAVNEMCNLITGYLTQNHGFPVITR
                     RIELYSAKQKGVDYTDVQKAYSAMEAGLLQLAYPDKIAQAHAKFNEALGVYNQILQES
                     NPNDRKARVDANVTSACLVNMTEAYIWLNDYTNAEMTANRVINIGVNKYVRDARAHLE
                     FIRIQQTRWNANQY"
BASE COUNT         1438 a         1066 c         1200 g         1622 t
ORIGIN      
        1 gggggtaatg atcctctagc ccccaaaccc taaacacgta ctaaacgcga tttttgttta
       61 cctattgaaa atcaataggt aataaggcct tcaaaatttc actttttaat agcaattcat
      121 taaattgcag tgttaaattt tggtacaatg aatcgattct acgttcgctc attcacagtt
      181 gcaactgcct tgtttctatt tggaaatgca gttgcaggac ctgttgcaaa taccgttacc
      241 tcagcaggat ctgccgttat cgctcaggtt gcccgtttta atgtggtaaa cctgggtccg
      301 caagtggaga tttcctgggt tggtattaag gagaaagatg cttctttcta taccgttgag
      361 cgttcttccg atggagtaaa cttcattgaa atcacaaggg tgagcgctgc aagtgagtcg
      421 cctgcattac tcgaatattt tgaagccgat cgcgatccac tgccgggtac gtcttattat
      481 cgccttaagc aaacactcaa atcaggagag tacctctatt ccggacagat caccgttcgt
      541 cgtcaggact gcggttctga gttaatgggt atgaatgccg gtacagaaat ggatccggta
      601 atgttccttg catctgccgc ttttggcaat gaagaggttc ttgttgtggt acgtgacaga
      661 gagggcaaag aatttttctc caagatcatg ctcaccttcc tcgatggaaa gattgttggc
      721 tttgatccag acggacgaat tgttgatggc gattatatca ttatagcttc ctctaacgat
      781 ttgcttcaac agaagaagat taagatggat tgatctcctg tattcttcaa tcacctcaaa
      841 taggcggtat ttatcttaaa acgcgcattt tcctgtagag gaggaagata gttttaattt
      901 atctcagcag acatttctac tcaagaaaca ttatcgtgcg ttgatttcgt actttcgccc
      961 ctatgatttc agtcaggggt cttacaaaac gttatggaga gtttgctgca gttgaccatc
     1021 tcaacctccg ggttgagaag ggtgaatttt acagtttgct gggtcccaat ggtgccggaa
     1081 aaacaaccac catcaacatg cttaataccc tgctggatcc cagtgaagga tcggtcagta
     1141 ttaacggatt atctctgaat gcagatcgaa attccattcg cagacagatt ggcgttgtac
     1201 cacaagaaat ctccttgtat gatgaattaa gcgcattcga caacctgcgt ttttttggct
     1261 cactttacgg tataacggag tccctgttaa gagtgaggat tcaccattta ctggaggaat
     1321 tcggactcat tgaccgaatg aatgaaaaaa taaaacacta ttccggcgga atgaaacgtc
     1381 ggataaatat tgcatctgct ttattgcatc aacccgcatt ggtttttatg gatgaaccaa
     1441 cagtaggcat tgatccgcaa agcagaaata atatttacga ggccattcaa cggttgcgca
     1501 aagatggagt gacgattttg tataccaccc actacatgga agaggcagag cggttttcag
     1561 atcgtattgg aatcattgat cacggtacca tcatcgcaga aggcaccctc gcccagttgt
     1621 acacgcgtgc cggtcatggc gattcgatgg tgattgagct ggcagaaatc aatgagaaac
     1681 agtttataca aattcaaaac ctcaatggag ttgtcgtaag ccgtaccgaa aaccggattc
     1741 agtttcacgg cgacgattta cggaaaaaga ttccttccat tatgttgatg attacggaac
     1801 ttggattaga tctgatccat ctcgatattc ggaaacccga tctggaagca gtgtttcttt
     1861 cacttaccgg aaaaaacctc agagactgat gtttaaaata gccagaaaag atctcatgtt
     1921 gtttttcaac gataagaagg cagtcctgct cacctttctt atgcctatgg gattgatcac
     1981 gcttttcgta tttgcatttg gaggcaatca gaaaaagcgc gacatgaatg aaatgcgctt
     2041 attggtagca gatgaagatc aatcgaaaga ttcaaaagag cttgttgcgc aattggatgc
     2101 tcttcccaca cttcgaattg aacaaacaga ctgggaaaaa gcaaaacaac acgtttcctc
     2161 cggcgaacgt tcttcggcat tgcgcatttt tccttcttat tcggattctc ttcaattggg
     2221 agtttccgga tttggatttt atttcgatca ggcccgtcaa actgaagcgg ccatgattca
     2281 gggtgttctt tccggatttc ttttcgctca ggaaggcgat aaagtgatga ggggatcgtt
     2341 gggaactcaa cttgaaacgg aattcggaat ggaagacagt tcttctcggg aaatggtgtt
     2401 gatgatggtg gagcagatga tcgcaaaaaa ttcgatgcag gatccgtctg tttcgatgat
     2461 gcgattagaa gaagtgcgat ctgatgaagg agtgaatcca actcttgttc atgctgtagg
     2521 cggaacggcg gttatgacac ttttattttc tgttgctgca atgggtgcag gattgcttga
     2581 agaaaaggaa aaaggaacac ttcggaaatt attgctggca cccattcatc ctattcaaat
     2641 tttattcggt aaattaatct ctgccgttgc tattggtttt attcagttat ggttgatgct
     2701 gatatttgcc tggttggttt tcggacttca aatccttccg catattcctt ccctttttct
     2761 gatcatcttt tttactgcat tatgctgcgg ttcatttggt gttttcctgg cctctttttg
     2821 cactacgcga aaacaggtgg agggtttatc taccattatc gtgctcgtaa tgagtgcaat
     2881 tggcggatcc atgattccaa ctttttttat gccggagttt atgcagcagc tttctgtcct
     2941 ttccgtgaat tattggtcca ttcaggcgtt ctacgatgtt ttttggagag atgccgaatg
     3001 gatggtcttg tttttcaagt taatgatgtt gttttcaatc agcctggtga tgattgccat
     3061 tgctattcct ctttataaac gaaatatttt acggttgtcc tgaacatttc aaatcgtacc
     3121 ttgttacaag ctgtacagtt atgaagaagt attccatacg cgatatcgaa aaattgacgg
     3181 gcataaaggc gcataccatt cgtatgtggg agaaacgcta taatattctc gagccggacc
     3241 gaacatcaac caatatccgt aaatacagcg atcaggaatt gaaacgcatc ctaaacatat
     3301 caattctcaa ttcctgtggt ttaaagattt cccgcattgc aggcttaact gacaatgatc
     3361 tggccgtaaa agtgctggag ctgcaaaagg aaaatgtctc tgaggatgtt ttcattgagc
     3421 gcatggtggt ggcaatgatt gaattgaatg aagagttgtt tgaggaggtc ctagatgagg
     3481 ccatagagaa aaaaggtttt gagcaaacaa tgcttctcat cgtttatcct ttcttgcaaa
     3541 aaataggcat actctggcaa actggaagta tcaatcctgc acaggaacat tttatctcac
     3601 acctcatccg ccaaaaatta atggtggcta ccgatgcact aaaagggaag aaagcagata
     3661 aggaaacgtt cattttgttc cttcctgttc atgagatgca tgaaattgga ttgctctttt
     3721 acaactacct gttgcgcgcc aggggttacc aggtggttta tcttggacaa gcagttcctt
     3781 tcgacgatct cgaaaaggtt gcaaagattc gtcctgcaaa atatatactc accttttttg
     3841 tttctcctgt gcctgaagat ggattgcagg agtatgtcac taagctgcat cacacctttc
     3901 ctaaaacaaa aattttcgtt acaggttttc aattaggatt ggaacaggat ttatcttatc
     3961 cggataacat cgttccggta aaagcagtaa gtgagttttt aacccacctc aaataaaaaa
     4021 agccggagta taaaccccgg ctaagaatta aatttttatt aaatcaatat tgattcgcgt
     4081 tccagcgtgt ctgttgaatg cggatgaatt caaggtgcgc gcgtgcatca cgtacgtatt
     4141 tgttcactcc gatattgatt acccgattag cagtcatttc tgcattggta taatcgttca
     4201 gccagatgta agcttctgtc atgtttacca gacaagctga tgttacattc gcatctactc
     4261 ttgccttgcg gtcgttcgga tttgattcct gaagaatttg attgtagact cccaatgctt
     4321 cgttgaattt ggcatgggcc tgagcgattt tgtcaggata ggcaagttgt aacaatcctg
     4381 cttccatagc cgagtaagct ttttgaacat cggtatagtc aacacctttc tgtttggcac
     4441 tgtaaagctc aattctgcgt gtaataacag gaaagccatg attctgtgtg aggtatcctg
     4501 taataagatt gcacatttca tttacagcag atcgctggcg ttcttcccag gttggtaatt
     4561 cattgtcgaa ccaccacaat tcaagctcag cctttgttgc aaattctttg gtgcgggtgc
     4621 tttgaaaggg ctctgtctct acaacacgct gattaaaaac agtgccacgt ccaggaacat
     4681 cgatgcgcat tgtaacagga tgacggtact ggatgacgta ggaatatttt tctgtaggtg
     4741 cttgtcctgt tttatccaaa cgaaccggac ctttttcaaa accatcaaaa gtgagtgtaa
     4801 ttctggtctg tgatcccgcg ttcagagtga ttccttccgg acgtatagca gcagtaattg
     4861 aagcaggaaa gatgtcttgc agaataaatg gttttttagg acgtaaagga ggattcattc
     4921 caggataagg cggctttacc ggaggctgag ctgctgtatt tcctgcagca acctgttttt
     4981 cccactgcac catgttgttg aggtgctgac gatcgatctg agcggtttgc atttcatacg
     5041 cattcatata tccttccaat gtaaccaggt agttctgacg ggcttgttcc caggctttca
     5101 gtgaatcatc gttacgctgt ttatagctca tctgcacatc caatgcgtag gttctggagt
     5161 tatctgttat tttaacaaga ggcaattggg tgtatttgaa agtcaccttg ttgtcgagaa
     5221 tgttttgagc tgaagcgctg agccagagca ggggaagggc aaaaagtaga aatttttgtt
     5281 tcatggtagg aagattttaa agggcacaaa gccaatatcg tgccat
//
LOCUS       DAOMYG010000213         5303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5360_length_5303_cov_9.404916, whole genome shotgun sequence.
ACCESSION   DAOMYG010000213 DAOMYG010000000
VERSION     DAOMYG010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5360_length_5303_cov_9.404916"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PK610_07920"
     CDS             complement(<1..544)
                     /locus_tag="PK610_07920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRJ35900.1"
                     /translation="MGLSAVIITFNEERNIARCIDSLYGVADEILIIDSGSADKTIEI
                     CKEKNVKWIQQIWLGYSAQKNFGNQQAVNDWILSIDADEAISSELKEAILQVKSNAPL
                     SVCSFNRLTNYCGTWIKHGGWYPDTKVRMFDRRKVKWEGEIHEQLIGFSSAEVKFLEG
                     DLLHYSYYSVDDHYRQTEKFT"
     gene            complement(587..1840)
                     /locus_tag="PK610_07925"
     CDS             complement(587..1840)
                     /locus_tag="PK610_07925"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRJ35901.1"
                     /translation="MEHQVKIGLFGFGCVGQGLYDVLSQSRGFRTTIKRIVVKDPSKK
                     RSLPASMFAYDKNEILNDPEINLVVELIDNAKDAYEIVSTALRNGKNVVSANKKMIAE
                     NFEELVRLQHEFGTSLLYEASSCGSIPILRNLEEYYDNELLRAVSGIFNGSSNYILTK
                     VQNEHLDYATALRQAQELGFAESDPTLDVEGFDAKYKLVIITGHTFGVFARPEDVLNL
                     GIGNLAVGDVEFAQSRGKRIRLVANTFQLDDQRISLFVTPRLVAKDDQLYNVNNEYNA
                     VILEAAFSDKQLFVGKGAGGHPTGSAVLSDISANAYHYRYEYKKYHQQARFTYSTEIE
                     LEVYLRSNDPVLFDEIGFTSISERGINYVSGVNYVIGTIGLKDLIRHRERINASGSFL
                     AATGEVIVLDGSRKERKQLSESVGI"
     gene            2085..4082
                     /locus_tag="PK610_07930"
     CDS             2085..4082
                     /locus_tag="PK610_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015024636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ35902.1"
                     /translation="MGKEPILEVRNLVTEFTSDGVVTKAVNDVSFTLHKGETIGIVGE
                     SGSGKSVTSLSIMRLIPSPPGRITGGQIIYHGQKETIDLLQVSEKEMQSHRGNDIAMI
                     FQEPMTSLNPVLTCGEQVAEAILRHERFPELSRFRLFLENNKNLFFIASSILFLIAFV
                     ILVSGEFNQIIGWVSLIAGFSLTLTGIYLSIFSKSRAVAKGRVIDLFKQVKLPRPERV
                     YDAYPHQLSGGQKQRVMIAMAMSCNPGILIADEPTTALDVTVQKTILDLMNDLQAKNN
                     LGILFITHDLGVIAELADKVVVMYKGKIVEQGSVLDIFTNPQHPYTKGLLACRPKLNN
                     RLRSLPTVSDFMNTDEQGNITETNVTIQSVIHNLIEDPYQRKSEQEKLYASEPILKVQ
                     NLKTWFPVGRNMFGKVSDYIKAVDDVTFDVYPGETLGLVGESGCGKTTLGRTILRLVE
                     PHEGKITFQGKDITFISQQEMRPIRKDIQIIFQDPYSSLNPRMTIGEAIMEPMRVHGI
                     LDTDKARKNRVVEILERVNMRPSHFSRYPHEFSGGQRQRICIARAIALQPKFIICDES
                     VSALDVSVQAQVLNLLNELKREFGFTYIFISHDLSVVKFMSDRMIVMNKGKIEELDLA
                     ERIYADPKSDYTRKLINAIPKGELDDIRKNIENKLLRENHA"
     gene            4196..>5303
                     /locus_tag="PK610_07935"
     CDS             4196..>5303
                     /locus_tag="PK610_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35903.1"
                     /translation="MDQGDGMKSYYVMKYHIENDPSYLQFTGMNYPYGEHYGFTDGMP
                     AFTWLFQTVPALQAYAIPIIHLSLFANLVLCALFVFLILKKFEISQWFAAIASIGILI
                     LQPQIHRLTGHPSLSFASFFPIAWYILICYLENPSRFLKILLIINPLFWFFIHAYLGF
                     MIVLFYLFSFIVIGITQREKGLKSQLWNIGFSVLPVIIFFSFIKLTDTITDRPTDPTG
                     LFDYTASMRSVFLPSHPPLNTFFHSMFESTNPGSWEGWSYIGITATVAVFAVFLSLFI
                     QLFKTRSIQSAISNTVPFHVRPYILPAILILLFSFGIPFNIFPKLLDYITPVKQFRGL
                     GRFAWVFYYVSTLLSVTILYYWWKKSRTENKLKRI"
BASE COUNT         1524 a         1178 c         1013 g         1588 t
ORIGIN      
        1 aggtaaattt ttctgtttgc cggtaatgat catcaaccga ataataggag taatgcagca
       61 aatctccttc gagaaatttc acttcagcag aggagaatcc aatgagttgt tcgtgaattt
      121 caccttccca ttttactttt ctccgatcaa acattctgac tttcgtatcc ggataccaac
      181 ctccgtgttt tatccaggta ccgcagtaat tggtcaaccg gttaaatgaa caaaccgaga
      241 gcggtgcatt cgattttacc tgaaggatgg cttcttttaa ttccgaggaa atagcttcgt
      301 cagcatctat cgacagaatc caatcgttga cggcttgttg attaccgaaa tttttctgtg
      361 cagaatatcc cagccagatt tgttgaatcc actttacatt tttctcctta caaatctcaa
      421 tcgttttgtc ggctgatccg gagtcgataa tcagaatttc atcggcaaca ccatacaaac
      481 tatcgataca gcgagcgata ttgcgttctt cgttaaaggt gataatgaca gctgaaagac
      541 ccatgacagt ctttcaaaga taaagctttt ctgcacctgt tcagattcag atgccaacgc
      601 tttcagataa ttgtttgcgc tccttacgag agccatccaa aacgatcact tctccggtag
      661 cagcaagaaa tgatcctgat gcgttgatcc tttcacgatg acgaatcaga tccttgaggc
      721 cgattgttcc gatcacataa ttcacaccgg agacataatt gatccctctt tcggaaatcg
      781 aagtaaatcc aatctcatca aacaaaaccg gatcattact acgcaggtaa acttccagtt
      841 caatttctgt ggaataggta aatctcgcct gttgatggta ttttttatac tcgtatcggt
      901 aatggtaggc attggcagaa atatcagaca atacagcact tccggttgga tgtccgcccg
      961 cacccttgcc cacgaacaat tgcttatccg aaaaagctgc ttccagaatt acagcattgt
     1021 actcattgtt cacattgtac aattgatcat cttttgctac aagtcgggga gtaacaaaca
     1081 aagaaatacg ctgatcatcg agctgaaacg tgtttgcaac aagtcgaatt cgctttcctc
     1141 ttgactgcgc aaattctaca tcacccactg caagattacc aattcccaga ttcagcacat
     1201 cttcaggtcg tgcaaataca ccgaaagtgt gacctgttat gataaccaat ttgtatttcg
     1261 catcaaatcc ctcaacatcg agtgtaggat cactttccgc aaagccaagt tcctgcgcct
     1321 gacgcaaagc cgttgcataa tccaaatgtt cattctggac tttagtaaga atatagttgg
     1381 aggaaccgtt aaagattccg cttacagcac gcaacaattc attatcataa tactcttcaa
     1441 gattccgcaa aataggaatt gaaccgcagg aagaagcctc atacaacagc gaggtgccaa
     1501 attcatgttg cagacgcacc agctcttcaa aattttcggc aatcattttc ttatttgcac
     1561 tcactacatt tttaccatta cgaagcgcgg ttgaaactat ttcgtatgca tcctttgcat
     1621 tgtcgatcaa ttccaccacc agattaatct caggatcatt gagaatttca tttttatcgt
     1681 aggcaaacat cgatgccggt aaacttcttt tttttgaagg atctttcaca acaatacgct
     1741 tgatggttgt tctaaagcca cgactttgcg aaagcacatc gtagagacct tgtcccacac
     1801 aaccaaatcc gaacaaacct atcttaacct gatgctccat agtctaattt tagcgggtca
     1861 aaaatagcat gctacctcgg ccttcccaaa gaaatacagg caaataagct gtcttttgcc
     1921 ctattaaaaa cagcccggaa ttgaaatcct gaagatccgt cgcttgccta tatggggtgg
     1981 tcgaaggaaa ctactcgctg agcgagaggg ggcccgaata caaaaagatc attgcctgag
     2041 tcattaaaac accttatttt tagcctcttg agaattaatc ccccatggga aaagaaccta
     2101 tactggaagt gcgcaacctg gttaccgagt tcaccagcga tggtgttgta accaaggcag
     2161 tgaatgatgt ctcctttacc ttacataagg gagagactat tggaattgtt ggagaatccg
     2221 gctcaggaaa gtcggttact tccctttcca tcatgcgcct tattccctca cctcccggaa
     2281 gaattacagg aggtcagatc atttatcacg gtcaaaaaga aaccatcgat ctgcttcagg
     2341 tttcagaaaa ggaaatgcaa tcgcatcgcg gaaatgacat tgccatgatc tttcaggaac
     2401 cgatgacctc actcaatccg gttctcactt gtggagaaca ggttgcagaa gccattttgc
     2461 gtcacgaacg cttcccggaa ctctcacgct ttcggctatt tctggaaaac aataaaaacc
     2521 tctttttcat tgcttcttcc attctgtttc tgatcgcatt tgtaattctt gtatccggcg
     2581 aattcaatca aataatcgga tgggtttcgc tgattgccgg attctcgctt acgcttaccg
     2641 gaatatatct ctccattttc tccaaaagca gggccgttgc aaaaggaagg gtaatcgatc
     2701 ttttcaagca ggttaaactt ccacggcctg aacgcgtgta cgatgcctat ccccatcagt
     2761 tatctggcgg acaaaagcag cgtgtgatga ttgcgatggc tatgagttgc aatccgggaa
     2821 ttctcattgc agacgagcct actacagccc ttgacgtaac cgttcaaaaa acaattctcg
     2881 atctgatgaa tgatcttcag gcgaagaaca atcttggtat tctctttatt acccatgatt
     2941 tgggtgttat tgcagagctt gcagacaagg tggtggtgat gtacaaagga aaaattgttg
     3001 aacaaggaag cgtacttgac atattcacca atccgcaaca tccgtataca aaaggacttc
     3061 ttgcctgtcg tcctaaattg aataaccgtc ttcgttcgtt acctacggta agtgatttta
     3121 tgaataccga cgagcaaggc aacattacag aaacgaatgt tacgattcag tcggtgattc
     3181 acaatttaat tgaagatccg tatcagcgaa agagtgaaca ggaaaaactt tatgcatctg
     3241 agccaatctt aaaagttcag aatctgaaaa catggtttcc tgtaggcaga aacatgttcg
     3301 gtaaagtgag tgattacatc aaggcagttg atgatgtgac ttttgatgtg tacccggggg
     3361 aaacattggg tcttgttgga gaatcaggtt gcggaaaaac aactttagga agaacaattc
     3421 ttcggttggt agagccccat gaaggaaaaa ttacttttca gggtaaagac atcaccttta
     3481 tttcacagca ggagatgcgt ccgatacgca aggatattca gatcatattt caggatccct
     3541 attcctcatt aaacccgagg atgaccatag gtgaagccat tatggaaccc atgcgcgttc
     3601 atggaattct tgacacagat aaggcaagaa aaaaccgcgt ggtagaaata ctggagcggg
     3661 taaatatgcg cccttcccat ttcagtcgtt atcctcatga attttccggc ggacaacgcc
     3721 aacggatttg cattgcgcga gcgattgcat tgcaaccaaa attcattatt tgcgacgaat
     3781 ccgtttctgc actggatgtt tctgtacagg ctcaggtttt gaatctgctg aatgaattga
     3841 agcgtgaatt tggatttacc tacatcttca tttcgcacga tttgagtgtt gtaaaattca
     3901 tgagcgaccg gatgattgta atgaacaaag gaaaaattga agagcttgat cttgccgaac
     3961 gcatttacgc cgatccgaaa tcagactaca cccgtaagct aatcaatgcc attccgaaag
     4021 gagaattgga tgatatccgg aagaatattg agaataaact tctaagagaa aatcatgcct
     4081 gatggtaggt tttctttata aaaaatcagc ttctctgttc gtcgtatttg gatcgacaat
     4141 ctttcttctt tctttttaca gtcacattat tttttcccca aattcatatt tgttaatgga
     4201 tcaaggggat ggaatgaaat cgtattatgt gatgaaatat cacatagaaa acgatccgag
     4261 ctacctgcaa ttcaccggaa tgaattaccc ttatggtgaa cattacggct ttaccgatgg
     4321 aatgcctgct tttacctggc tatttcaaac agtacccgca cttcaagctt atgccattcc
     4381 aatcatacat ctaagcttat ttgcaaacct tgttttatgt gctctgtttg tttttttgat
     4441 tcttaaaaaa tttgagatca gccaatggtt tgctgccatt gcatcaatag ggattctcat
     4501 tcttcaacca cagattcacc gtttaaccgg ccatccttcc ttatccttcg cttccttttt
     4561 tcctattgcc tggtatattc tcatttgtta cctcgaaaac ccgagtagat tcttaaaaat
     4621 acttttgatc attaatcccc tattctggtt tttcattcat gcctacttag gctttatgat
     4681 tgtattgttc tatctttttt catttattgt aattggaata acacaaagag agaaaggatt
     4741 gaaatcacaa ctttggaata ttggattctc tgttctgccg gtaattattt ttttcagctt
     4801 tattaaatta accgacacga tcacagaccg tccaacagat ccaacaggtt tgtttgacta
     4861 tacagcatcg atgcgatccg tctttttgcc aagccaccca ccgttaaata cattttttca
     4921 ctcaatgttc gaatcaacca accccggctc ctgggaggga tggtcctata ttggtattac
     4981 tgccactgtg gctgtttttg cagtttttct ttcgctattc attcaattat tcaaaacccg
     5041 atctattcag tctgcaatca gcaacaccgt gccttttcat gtcaggccat atattcttcc
     5101 ggctattctc atcctgttat tttcttttgg aattccattc aatatatttc caaagcttct
     5161 tgattacatc acgcctgtaa aacaattccg tggactggga agatttgcct gggttttcta
     5221 ttacgttagc acccttcttt ctgtaacgat actttactat tggtggaaaa aaagcagaac
     5281 tgaaaataaa ctaaaaagaa ttg
//
LOCUS       DAOMYG010000214         5302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5365_length_5302_cov_9.235563, whole genome shotgun sequence.
ACCESSION   DAOMYG010000214 DAOMYG010000000
VERSION     DAOMYG010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5365_length_5302_cov_9.235563"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PK610_07940"
     CDS             complement(<1..396)
                     /locus_tag="PK610_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35904.1"
                     /translation="MKKTATIISIGIPFLWLGFILAISFMEAPLKFTAPGIDLKLGLG
                     IGRIIFQTLNKIELFFLAALFSTFFSYQHKKKIVILLNILAVILLYQTFYLLPELDKR
                     AIQLLNNTDPGPSSLHFIYILLDAIKVLLL"
     gene            complement(393..1172)
                     /locus_tag="PK610_07945"
     CDS             complement(393..1172)
                     /locus_tag="PK610_07945"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRJ35905.1"
                     /translation="MFFPVALSLGLLGSFHCVGMCGPIALALPLNKKNYLTMTTGGLA
                     YNLGRVFTYSLIGLVFGLIGQGLFIAGYQNFLSVILGVFLLVSLIVGTLALRSKTNSY
                     AFRIVNGIKARFIPLFQKKSTGALFLIGSLNGLLPCGFVYLGLAGAMALGDAFKGSMF
                     MVGFGLGTIPAMLAVNLLGGIIGTKLRNSIRKLSPVVIGLMAVILILRGLNLGIPYLS
                     PELKEQKIEVVLENGEKNESTLIVPECCSKPKKKNEVKIDP"
     gene            complement(1175..1612)
                     /locus_tag="PK610_07950"
     CDS             complement(1175..1612)
                     /locus_tag="PK610_07950"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRJ35906.1"
                     /translation="MSWGYRIVILYVGFVAIIITLVSMSMSQRLDMVTPNYYQEELQF
                     QGKIDRREAARSLKEPLSWVVEEKSIRLLFPKEFSGQEINADITFFRPSDARQDKKMS
                     IKGTELVQEISIEGMEHGYWEMKIEWRAGEKAFYDETSFRINK"
     gene            complement(1609..3030)
                     /gene="ccoG"
                     /locus_tag="PK610_07955"
     CDS             complement(1609..3030)
                     /gene="ccoG"
                     /locus_tag="PK610_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HRJ35907.1"
                     /translation="MNEEHQSDWDYRDESFRDSIGTIKDGKRNWVFPTQPRGRLYSAR
                     KILSYFYLLVFFSLPFIKYNGHPLLMLNVLERKFIIFGYIFWPHDFFIFGIAMLTMIL
                     FVVLFTVVFGRLFCGWACPQTIFLELVFRRIEYLIEGDSSAQKALNKMPWNNYKIIRK
                     SLKFFLFFLLSFIISNTFLAYIIGADELFKIISEPVGQHFGGFIALLLFTGVFYFVFA
                     WFREQACLIVCPYGRLQGVLLDKNSIVVAYDYVRGEQREKFRKGETRTKGDCIDCGLC
                     VRVCPTNIDIRNGTQLECTNCTACIDACDHVMENVGLEKGLVRYASEAGIANKEKLTI
                     NKRIIAYSSVLFLLLTVLVFLLVSRSDIEATIVRTPGQLFQEQPDERISNLYNIKVIN
                     KTSNEIPVRLKLENVEGEIQMVGDAIKVDKESNTQSTFFVILPKSVVTERKTEIKIGV
                     YSGEKRIEMIKTSFLGPIKKKKK"
     gene            complement(3032..4213)
                     /locus_tag="PK610_07960"
     CDS             complement(3032..4213)
                     /locus_tag="PK610_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HRJ35908.1"
                     /translation="MKPNLFHKLILISGILFLPALSIAQDVNRPTVPDFDSTIYTNPV
                     FIALLASAILLMILIMVLSDILKSSLHHQREREKKKSGGTQAIMIALLLLAPALTQAQ
                     DIATTASGVEIPRISNDWYGMDGFTFWALVLFNLFELVVVFFFLSQIYSLLNSPAKAK
                     AIAERKTVSIIDRLNASVSLEKEKDILLDHNYDGIQELDNDLPPWWKYGFYVTIIAAF
                     AYMTHYHILKTGALQEEELANEFALAEKQQEEYRKKNANMIDENNVTFLTDESSINEG
                     RGIYVKKDCKACHGIAGEGTTIAPNLTDDYWIHGGGITDVFKSIKYGWTEKGMKAWGG
                     ELTPREMHVLSSYVKSLKGSNPAGAKAPEGVLFTEEGGSAATDTTENTTAITPSADTL
                     K"
     gene            complement(4242..4415)
                     /locus_tag="PK610_07965"
     CDS             complement(4242..4415)
                     /locus_tag="PK610_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HRJ35909.1"
                     /translation="MKFINYLTSIDGVGIFPVVSLIIFFTFFIALLVYIMKVDKKLIS
                     HMEQLPFEDNDQQ"
     gene            complement(4418..>5302)
                     /gene="ccoO"
                     /locus_tag="PK610_07970"
     CDS             complement(4418..>5302)
                     /gene="ccoO"
                     /locus_tag="PK610_07970"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HRJ35910.1"
                     /translation="NQILPMYAMRAIGGTMFLTGAIMMTYNLIKTVRKGNFLADEEAE
                     APALEKTYHKHKGEHWHRWIERKPVQMLVVSLFVILIGGLVEMIPTFLIKSNIPTISS
                     VKPYTPLELEGRDIYVREGCYVCHSQMVRPFRDEVARYGEYSKAGEFVYDHPFQWGSK
                     RTGPDLAREGVGNNKKSNAWHYNHMLDPAVPSPGSIMPPYPWLFDKHVDLEMLPKKIR
                     AMQTLGVPYPEGYDLIAANEFMAQADSIVADLEKNGLPAESTNEIVALIAYLQRLGRD
                     IYNAPKTDKTPVILTEKE"
BASE COUNT         1506 a         1124 c         1040 g         1632 t
ORIGIN      
        1 aagtagtaaa acttttattg catcaaggag aatatatata aaatgaagtg atgatggtcc
       61 gggatcagta ttattgagca gttgaatggc tcgcttgtca agttccggca acaggtagaa
      121 ggtttgatat aggagaatga cggccaaaat atttaggaga ataacaattt tcttcttgtg
      181 ttggtaactg aaaaaagtgg aaaagagggc agcaagaaaa aacaattcaa ttttattcag
      241 ggtctggaaa ataatccgac caatacccaa tccaagttta agatcaatgc ctggtgctgt
      301 aaattttaat ggtgcttcca taaaagaaat ggccaggata aatcccagcc ataaaaacgg
      361 aattccaatg gaaatgatag ttgccgtttt tttcacggat caatctttac ttcgtttttc
      421 tttttgggtt tgctgcaaca ctcagggacg attaatgtag actcattttt ttctccattt
      481 tccagaacca cttctatttt ttgttctttc aattcaggtg ataagtacgg gattcctaaa
      541 tttagtccgc gaagaataag aatcaccgcc attagtccaa ttaccacagg cgataatttc
      601 cgtatagagt tgcggagctt cgtaccgata attcctccca ataaatttac tgccagcata
      661 gcgggaattg ttccaagacc aaaaccaacc atgaacatac ttcctttaaa tgcatcgcca
      721 agtgccattg ctcctgcaag tcccaaatag acaaaaccac agggcagcag tccgtttaaa
      781 ctgccgatca aaaacaaggc tccggttgat tttttctgaa acagaggaat gaagcgtgcc
      841 ttaatgccat ttacaattct aaaggcatat gaattcgttt tagaacgcaa agccaatgtt
      901 ccaacaatca gacttaccaa tagaaatact ccaaggataa ctgagagaaa attctggtat
      961 cctgctatga acagaccctg cccgattaat ccgaatacaa gcccgataag cgaataagta
     1021 aatactctac caaggttgta ggccaatccc ccggtggtca tggtcaggta attttttttg
     1081 tttagcggta atgccaaagc aatcggccca cacattccaa cgcaatggaa gctgcccaat
     1141 aaacccagtg ataatgctac aggaaaaaac atgtttactt gtttattctg aatgatgttt
     1201 cgtcataaaa cgctttttct ccggctctcc actcaatttt catttcccaa taaccgtgtt
     1261 ccattccttc tatcgatatt tcctgcacaa gttctgtgcc tttaatactc atttttttat
     1321 cctgacgcgc atcactgggg cgaaagaagg tgatgtcggc attgatttcc tgtccgctga
     1381 attcttttgg aaaaagtaga cgaatgctct tttcttccac aacccagctt aagggttctt
     1441 tgagcgaacg tgcagcctct ctgcgatcaa tcttaccttg aaactgcaac tcttcctggt
     1501 agtagttcgg tgtgaccatg tccagtcgct gactcatgct cattgatacc agcgtaatga
     1561 tgatggctac aaaaccaaca tacagaataa cgatgcgata accccagctc attttttctt
     1621 ctttttaatt ggacctaaaa aacttgtctt tatcatttca atcctttttt cacctgagta
     1681 tacaccgatt tttatttcgg tttttcgttc cgtaacaact gatttcggaa gtattacgaa
     1741 aaaagtggat tgtgtatttg attctttatc tactttaatt gcgtctccca ccatttgtat
     1801 ttctccctcc acattctcaa gtttcagtcg gacgggaatt tcgtttgatg ttttattaat
     1861 gactttaatg ttgtataggt tactgattcg ttcatccggc tgctcctgaa ataattgccc
     1921 gggagtcctg acaattgttg cttcaatatc acttctgcta accaataaaa acaccaatac
     1981 agttaataga agaaagagaa cagatgaata agcaattatt cttttattta ttgtaagttt
     2041 ttctttattc gcgattcctg cttcagatgc ataccgcaca agtccttttt ccaaccctac
     2101 attttccatc acgtgatcgc atgcgtcaat acaggccgtg caattagtgc attccagctg
     2161 agttccgttt ctaatatcga tgttggtggg acaaacacgt acacacaatc cgcaatcaat
     2221 gcaatcgcct tttgtacggg tttctccttt tctgaatttt tcacgctgtt ctcctcttac
     2281 atagtcgtat gcaactacaa ttgaattttt gtcgagtaaa acaccttgca atcgtccgta
     2341 aggacaaacg atcaaacagg cttgttcgcg aaaccaggca aatacaaaat agaagacacc
     2401 tgtaaataat aacaatgcga tgaatccgcc aaagtgttgg ccaacaggtt cagaaatgat
     2461 cttgaataat tcatctgcac caattatata agccaaaaaa gtgttggaga tgatgaaact
     2521 caataagaag aacaaaaaga attttagcga tttgcgtata atcttataat tgttccaggg
     2581 cattttattc aatgcttttt gcgcactcga atctccttca ataaggtatt cgattctcct
     2641 gaaaaccaat tccaggaaaa ttgtttgtgg gcacgcccaa ccgcaaaata acctgccgaa
     2701 tactacggta aacagaacca caaaaagaat cattgtcagc atcgctatgc cgaaaatgaa
     2761 gaaatcatgt ggccagaaaa tgtaaccgaa tataatgaac ttccgctcca gcacgtttag
     2821 catgaggagc ggatgcccgt tgtattttat aaagggtaat gagaagaata ccagcaggta
     2881 aaaatagctg agaatttttc gtgcagaata caaccttcct cggggttgtg ttgggaagac
     2941 ccaattcctt ttcccgtctt ttatcgtacc tatggagtct ctgaaagatt catcacgata
     3001 atcccagtcg ctttgatgtt cttcgttcat tttattttag cgtatcagca cttggtgtaa
     3061 tcgcagtggt gttttcggtt gtatctgttg ccgcacttcc tccttcttca gtgaatagta
     3121 ctccttcggg tgcttttgct cctgctggat tactaccttt caaagattta acgtagctgg
     3181 aaagtacgtg catctcgcga ggggttaatt cacctcccca tgctttcatt cctttttcag
     3241 tccaaccgta tttgatggat ttaaatacat ccgtaatgcc accgccatgg atccagtaat
     3301 catctgttaa attaggagca atggttgttc cttctccggc aataccgtga cacgctttac
     3361 aatctttctt gacataaatt ccacgccctt cattgatcga ggattcatcg gtaagaaatg
     3421 taacattgtt ttcatcgatc atgttagcat tcttcttgcg gtattcttcc tgttgctttt
     3481 ctgccagagc aaattcattg gccaattctt cttcctgaag tgcgcctgtt tttagaatgt
     3541 ggtaatgtgt catgtaggca aatgctgcaa ttatagtcac ataaaaacca tatttccacc
     3601 atggcggaag gtcattgtca agttcctgaa ttccgtcgta gttgtgatcc aaaagaatgt
     3661 ctttttcctt ctcaagcgaa actgatgcat tcagacgatc aattattgat acagttttcc
     3721 gctcggcaat agctttggct tttgcaggac tgttgagtaa actgtaaatc tgacttagga
     3781 aaaagaaaac aacaacaagc tcaaataaat tgaataatac caaggcccaa aaggtgaatc
     3841 catccattcc gtaccaatca ttgctgatcc gtggaatttc aactccgctg gctgtagtag
     3901 caatatcctg tgcttgtgtt agtgcaggtg ccaaaaggag aagcgcaatc attatcgctt
     3961 gtgtgcctcc tgattttttc ttttcacgtt cacgttgatg atgaagtgat gactttaaga
     4021 tgtcggaaag gaccataatt aaaatcatca gtagtattgc tgatgccaaa agtgcgataa
     4081 aaaccggatt ggtataaatc gtactgtcaa agtccggaac tgtcggtctg ttgacgtcct
     4141 gcgcaataga aagtgcaggc aaaaacaaga ttccgcttat taggatcagt ttgtgaaaca
     4201 ggtttggttt catagctgta agttgtctgt tcataatccg tttattgctg gtcattatcc
     4261 tcaaagggta attgttccat atgactgata agtttcttgt ctactttcat gatatacacc
     4321 agtagtgcaa tgaagaaagt gaagaagata attagtgaaa ccacgggaaa gatgccaacg
     4381 ccgtctatcg aagtgaggta gttgatgaat ttcatactta ttctttttct gtaagaatta
     4441 ccggtgtctt atctgtcttc ggagcattgt aaatatcacg tcccaatcgt tgcagatagg
     4501 cgatcagtgc tacaatctca ttggtgcttt ctgcaggtag tccatttttt tcaagatcag
     4561 ctacaatgct gtctgcttgt gccatgaact cgtttgcagc aatgaggtca taaccttctg
     4621 gataaggaac tcccaatgtt tgcattgcac ggattttctt tggaagcatc tccagatcca
     4681 catgtttatc aaacagccat gggtaaggtg gcataatgga tcccggagaa ggtacggccg
     4741 gatcaagcat gtggttgtag tgccatgcat tcgatttttt attgttgcca acaccttcgc
     4801 gcgccaaatc cggaccagta cgttttgatc cccattggaa ggggtggtcg taaacgaatt
     4861 cacctgcttt ggagtactca ccgtaacgtg ctacttcatc gcggaatggg cgcaccattt
     4921 gtgagtgaca cacataacaa ccttcccgca cgtaaatatc acgtccttca agttccaggg
     4981 gagtgtaggg ttttacactg ctaatggtag ggatatttga tttaatcagg aatgttggaa
     5041 tcatttctac taatccgcca ataaggatca caaataagct tacaaccagc atctgcacag
     5101 gcttgcgctc gatccaacga tgccagtgtt cacctttgtg tttgtgatag gttttttcca
     5161 gggcaggagc ttctgcttct tcgtctgcaa ggaaatttcc cttacgaact gttttgatta
     5221 agttgtaagt catcatgatt gcaccggtga ggaacattgt tcctccgatt gcacgcattg
     5281 cgtacatggg cagaatctga tt
//
LOCUS       DAOMYG010000215         5290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5381_length_5290_cov_9.835912, whole genome shotgun sequence.
ACCESSION   DAOMYG010000215 DAOMYG010000000
VERSION     DAOMYG010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5381_length_5290_cov_9.835912"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PK610_07975"
     CDS             complement(<1..290)
                     /locus_tag="PK610_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35911.1"
                     /translation="MLFWLVLFGIITHSIGYKFGLGFLMLYPEYLGEVGFASHAILGF
                     ALAGFIMAFNMYSYVMHGYKFSFIATVSKPFYKFSINNFIIPTAFILLYVYQA"
     gene            complement(448..1440)
                     /locus_tag="PK610_07980"
     CDS             complement(448..1440)
                     /locus_tag="PK610_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HRJ35912.1"
                     /translation="MDHLVPLSGQNLSLINLRKGETKLGETMLINGRDPKPGKGKAGF
                     AVIGIKEDIGPRANFGKPGADKMWECFLGKFLNLQSNQFLTGSTIWLAGSIDCSEEMK
                     LAEGTPTIERLRELTSQIDKKVAETIREIMEIGMIPVVIGGGHNNAYGIIKGVSEAKK
                     RSIGVINIDPHADFRPIEGRHSGNGFSYAFEEGFLSTYFVLGLHEAYNSAYIIDHFNS
                     NPALAYSSFDHLLRGETTLEIQIERALEHMDKTPFGLELDLDSIAGFPSSAETESGYT
                     VNEARKIIMALSKETNPLYAHFPEGSPDHGTDSAALHGKTLAYLVSDFIKSNTK"
     gene            1607..5260
                     /locus_tag="PK610_07985"
     CDS             1607..5260
                     /locus_tag="PK610_07985"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ35913.1"
                     /translation="MRQILLLILGVFFITRVDAITVVGQNGLITANFTANCAPVNVNF
                     TSTFVPGTVNYVWYFGDGSPSSTLASPSHTFQYAGLYTVQVAYYDASFNLLAEDYMYI
                     DMNGEPQSIQAYTTTACANDRLSFWVPNGANSNPQIGYSYTWNFGDGNVETSPYPSTD
                     HIYSSAGIYTLSVTCNTPCGTFVKNLTVTIGTNVPMTSGYFSIYPQEICPGDQVYVNY
                     GGSTTQFITFGDGTFTTNAYTKSYSQPGTYPVTATLTNGCGNTFTYFDTVRVRTDIGF
                     GPNSGISVYNSSPACIGMEVYFSGPSNYSNVVWNYPDGTSGFGPWDSQMVNGAFSTTP
                     VSVHITNGCGRDTTIYTTVQIVTTIPVTEIDLTVPASVCTGSELGFSANGDNEQEQGF
                     TYAWNFGDGTTSTQFGGTHVYSASGTYNVSLTVTNGCGNTATQTTTVTAGTNIAPDPN
                     TGLFAAPDDGACVGDSVLVVVYPGLNGTYSWDFGDGSNETGYSLITVFGRQYAYKKHR
                     YAATGSYDLSMTYTNSCGLSVTRTFTYNIGSNIEASANVLFDETQQICLGEPIPFSAL
                     GGSQYAWDFGDNTGVLVTNSTFVPIYHTYDNPGSYNVTLVVTNGCGNTETETFNVFVP
                     DNRINISTSTTYSQCGQDNGIAIAVIDGGFPPYDIRWSNGETTVVADSLSSGIYVVNV
                     EDSKGCYNFALASVNDAQAPNVVVNNVGNVTCYGNNDGFIDINVIGSTAPYSYQWSNG
                     ATTQDIGGLEAGPYEVIVTDANGCRAVRSITVSEPGQVTVSFVKEQASCGLNNGHIQA
                     IASGTTGPFTYIWSSGQSGPGLYNVGLGIYTVNVIDAAGCITTKSVAMSEASSVQGPT
                     FGGPAILVAGVSPLNCNGGGANIDIEVVQSSGNLSYNWNTGASTQDVTVTQVGQYNVL
                     VTDLTTGCKAIEIFTITHAAPDGQNICMVTVDSIYSYNKVVWEKEISTEISHYNVYRE
                     SSQNGLYFHIGTVPYDSLSVFDDYVANPMITSWRYKLAAVDFCGVESDRSDFHKTIHV
                     TQNLGLGGTINLIWDHYRGFGYSTYNVIRYTNSNGWETIATVSSANTSYTDPTPPSDP
                     GLFYIIEAVPSSPCVSTRAINNNTTRSNRTQNPVAPPASVGEMIGAIEGIAVYPNPND
                     GNFTLQADFVQAEDAAVEIWSLDGKVIRSFDVQGVTGRYTTSIQMQEVPQGIYILRMS
                     SASSSFVRKVVVY"
BASE COUNT         1462 a         1096 c         1089 g         1643 t
ORIGIN      
        1 gcctgataaa cgtagagtaa aataaatgct gtggggatga tgaaattgtt aatggagaat
       61 ttgtagaacg gttttgaaac tgtggcaata aatgaaaatt tatagccatg cattacgtaa
      121 ctgtacatgt tgaaagccat aatgaaacct gcaagagcaa atccaagtat ggcatgtgat
      181 gcaaaaccta cttctccaag gtattcagga tagagcatga gaaaaccaag tccgaattta
      241 tatccaatag agtgggtgat gataccaaac aaaaccagcc aaaaaagcat caggaaatga
      301 ttccttttta aatgaaggaa aagaagctga atgggaaaga agaaaaaagc tttccggata
      361 ccgctaaacc aactagtttt ttcttccatc agatgtagtg ctttcttttc attgatacgc
      421 ttgcttttgt ataaggttcc tcttttttca ctttgtgttg ctttttataa aatcactcac
      481 caggtaggca agtgtttttc catggagagc ggcagaatct gttccgtgat cgggtgatcc
      541 ttccgggaaa tgggcatata atggattggt ttctttcgaa agtgccataa taattttgcg
      601 tgcctcattc acggtatatc cactttctgt ctctgcactg cttggaaatc ctgcaattga
      661 atccagatcc agttccaacc caaacggagt tttatccatg tgttccagtg ctctttcaat
      721 ttgtatttca agcgttgtct ctccgcgaag caagtggtca aatgatgaat aggctagtgc
      781 aggattcgaa ttgaaatggt caattatata ggctgaattg taagcttcat gcagacccaa
      841 aacgaaatag gtcgaaagaa atccttcttc aaatgcataa ctgaatccat tgccactgtg
      901 ccttccttca atcggacgga aatcggcatg tggatctatg ttgataactc cgattgatcg
      961 ttttttagcc tctgaaactc ctttgattat tccatatgcg ttgttatgcc caccgccaat
     1021 taccacggga atcattccga tttccataat ttcccggata gtttctgcaa ctttcttgtc
     1081 tatctgtgag gtcagctctc ttaatcgttc aatagtgggc gtgccttccg ccaatttcat
     1141 ttcttcactg caatcgatgc ttccggcgag ccaaattgtt gatccggtta aaaactgatt
     1201 ggattgtagg tttagaaact tgcctaaaaa gcattcccac attttatctg cccccggttt
     1261 tccgaaattt gctctgggtc caatatcctc cttgatgcca ataaccgcaa atccggcctt
     1321 tcccttaccc ggcttgggat cacgaccatt aatgagcatg gtttctccaa gctttgtttc
     1381 cccctttctt aaattaatta agctaaggtt ttgacccgaa agtggtacta gatgatccat
     1441 ggcgttaaaa ttccttccta aatgtaaatt ttttatacgc attctggtca aggtttgaga
     1501 atttggagct gatttaactc attctttttc cggttaataa gttcatgatc aaattttatg
     1561 gcttaatggg gctattgtct aaattcgtcg tctaaatcat ttaaaaatga gacaaatttt
     1621 acttttaatt cttggtgtat ttttcattac aagggttgat gcgattacag tggtaggaca
     1681 aaacggtttg attaccgcca actttaccgc caactgcgcc ccggtaaacg tgaattttac
     1741 ctcgacattt gtacccggaa cggtaaatta cgtgtggtat tttggcgatg gttctccatc
     1801 cagtaccttg gcatcacctt ctcatacttt tcagtatgca ggtttataca cagtgcaagt
     1861 ggcatattat gatgcgtcct ttaatttgct tgcagaggac tatatgtata tcgatatgaa
     1921 tggggagcct caatccattc aggcctatac aaccacagct tgcgctaacg atcgtttaag
     1981 tttctgggtt cccaatggtg caaacagcaa tcctcaaata ggttattcgt acacatggaa
     2041 ttttggggat ggtaatgttg aaacctcacc atatccttca acagatcaca tttattcttc
     2101 tgcaggaata tataccttaa gtgttacctg caatactcct tgtggcacat tcgtaaaaaa
     2161 tctaacagtt actattggca ccaatgttcc aatgaccagc gggtattttt cgatttaccc
     2221 acaggaaatt tgtcccggcg atcaggttta cgtgaattat ggcggttcaa caacacagtt
     2281 cattacattc ggtgatggta cttttacaac caacgcatat accaaatcct attctcaacc
     2341 gggtacctat ccggttactg ctaccttaac gaatggatgt ggaaatacct tcacctattt
     2401 cgacactgta cgtgtccgta ctgatattgg gtttggtcca aattcaggaa tatctgttta
     2461 caacagttct cctgcttgta ttggaatgga agtttatttc agcggtcctt caaattattc
     2521 aaatgtagtt tggaattacc ctgatggaac atccggtttt ggtccatggg attcgcaaat
     2581 ggtaaacggt gcgttttcaa ctacacctgt ttccgttcat atcaccaatg gttgcgggcg
     2641 cgatactaca atttacacca ctgttcaaat tgtaactact attccggtaa cagaaattga
     2701 tttaactgta ccggcttccg tttgtacagg ttctgaactt ggatttagtg ctaatggaga
     2761 caatgaacaa gaacaaggat ttacctacgc ctggaatttc ggagacggaa ctacgtccac
     2821 tcaatttgga ggtacacatg tttattctgc aagcggtact tacaatgtat ctttaacagt
     2881 aacaaatggc tgtggaaata cagcaaccca aactactaca gttacagccg gaaccaatat
     2941 tgctcccgat ccaaataccg gattgtttgc agcgccggat gacggagctt gtgttggtga
     3001 ctctgttctt gttgttgttt accctggttt aaacggaacc tattcatggg attttggtga
     3061 tggttcgaat gaaactggat atagtttaat tactgttttt ggtcgtcaat atgcctataa
     3121 aaagcaccgt tacgcagcta ctggttctta tgacttaagc atgacctata ccaatagctg
     3181 cggtttgtca gttacgcgta cattcactta caatattggt tcaaatattg aagcatcagc
     3241 caatgtattg tttgatgaaa cccaacagat ctgccttgga gaaccaattc ccttcagtgc
     3301 attgggagga tcgcaatatg cctgggattt tggtgataac accggggttc ttgtgaccaa
     3361 ctcaacattt gttccgatat atcatactta tgataatccc ggaagttata atgttacact
     3421 tgtggtaact aatggttgcg gaaataccga aacagaaacg tttaatgtat ttgtgcccga
     3481 caacagaatc aacatttcta ccagtactac ctacagccaa tgtggtcagg ataatggtat
     3541 agccattgcg gttattgatg gtggtttccc accttatgat atccgctggt cgaatggtga
     3601 aactacggtt gttgccgatt cactttcttc cggtatatat gttgtaaatg tagaagacag
     3661 caaaggctgt tacaattttg ctcttgcctc tgtaaatgac gcacaggctc caaatgttgt
     3721 tgtaaacaac gtgggtaatg ttacctgtta tggaaataac gatggattta tagatatcaa
     3781 tgttattgga agcaccgctc cctattctta ccaatggagc aacggagcaa ccacacagga
     3841 tatcggtggt ttggaagcag gtccttacga ggtaatcgtt acagatgcca acggttgccg
     3901 tgcagttcgt tctattacgg tttcagaacc tggtcaggtt accgtttcat ttgtgaaaga
     3961 acaagcatca tgcggactaa ataacggaca tatacaggca attgcaagcg gtacaaccgg
     4021 acccttcacc tatatctgga gctcaggaca aagtggtccc ggattgtaca atgttggatt
     4081 aggaatttat actgtaaacg ttattgacgc tgcaggttgt attactacta agagcgttgc
     4141 aatgagtgaa gcaagttcag ttcagggtcc tacttttggt ggtcctgcta tacttgttgc
     4201 aggtgtttct cctttaaatt gtaacggtgg tggtgcgaac atcgatatcg aagttgttca
     4261 atcatccggt aatctgagtt acaactggaa taccggtgca tcaactcagg atgtaaccgt
     4321 tacacaagtt ggtcaatata atgtgttggt tacagaccta acaacaggat gcaaagcaat
     4381 tgagattttt accattacac atgctgctcc tgacggtcaa aatatttgta tggtaacagt
     4441 tgactccatt tattcctaca acaaagtggt ttgggaaaag gaaatttcaa ctgaaatctc
     4501 ccactacaat gtttaccgag aaagctcgca aaatggattg tatttccaca tcggaactgt
     4561 gccttatgat agcctgagcg tgtttgatga ctatgttgcc aacccaatga tcaccagctg
     4621 gagatacaag cttgctgcag ttgatttttg tggtgtagaa tccgatcgtt ctgatttcca
     4681 taaaaccatc cacgtaacac aaaacctcgg tttaggtgga actattaatc tgatttggga
     4741 tcattatcgt ggatttgggt attctaccta caatgtaatc cgttacacca attcaaacgg
     4801 ttgggaaact attgccactg tttcttctgc aaatacttcc tatacagacc caactcctcc
     4861 aagcgacccc ggacttttct acatcataga ggcggttcct tcaagtcctt gcgtttctac
     4921 acgtgcaatt aacaataaca ccacacgatc taaccgtaca caaaacccgg ttgcaccacc
     4981 tgcttctgtt ggagaaatga tcggtgctat tgagggaatt gctgtttatc ctaatccaaa
     5041 tgatggaaac tttacccttc aggccgattt tgttcaggct gaagatgctg cggttgaaat
     5101 ttggtcattg gacggtaagg taatacgttc ctttgatgta caaggggtta cgggacgata
     5161 taccacctct attcagatgc aggaagttcc gcagggaatc tacattcttc gtatgagcag
     5221 cgcctcttct tcctttgttc ggaaagtggt cgtttattaa gctgataatt actaatttca
     5281 aaccctcggt
//
LOCUS       DAOMYG010000216         5269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5412_length_5269_cov_8.768316, whole genome shotgun sequence.
ACCESSION   DAOMYG010000216 DAOMYG010000000
VERSION     DAOMYG010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5412_length_5269_cov_8.768316"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..586)
                     /locus_tag="PK610_07990"
     CDS             complement(113..586)
                     /locus_tag="PK610_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35914.1"
                     /translation="MEGLIIHTVAINRQGELKHFEIPLSNNAKFITGLWYKVRLITDA
                     ESGVVTELKSRSPYQPEVIIGELSLSSNQREGVFYFGKIILDNANYGLLDYTGGVFRV
                     NNYSHAKFPQSLKVKVDADTAIVHGYIHDNWGVISGRNVRYEIDLYIYQQLKEKP"
     gene            complement(592..984)
                     /locus_tag="PK610_07995"
     CDS             complement(592..984)
                     /locus_tag="PK610_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35915.1"
                     /translation="MEENELHSVPKVFEIQITAAGQKVSETFELDKSVKKVHGVAFSS
                     EKPDLMYHRGYARLEINNKEYFPDNHAVQRVMNGINANLDNRYWPLKKVIIGNGKVKI
                     EYTDKPDGRTVFAAYTVLLYFDCEIVID"
     gene            complement(1128..1847)
                     /locus_tag="PK610_08000"
     CDS             complement(1128..1847)
                     /locus_tag="PK610_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35916.1"
                     /translation="MLGQLSAIEEYNNAGALMGTEMMESEELNAAIKAMPMVQRAKTM
                     QKLVGNPFPSKGSRAEMEKHFPELQKHIKDGLGKGELRLADTVIYSIKAASSKTIKMF
                     ETQDDKEIGLRNISNAKLPKNQSMLVSGIILLAGVSADATKDKIIATNYDKIELFPAI
                     ANGEFSLKANKKQIVPDTSNNLFKTSNLNLVPMGYYKLSNPRLIQDDVLIELTIELGT
                     MDGIAANTYIFAGLHGTITTP"
     gene            complement(1984..2694)
                     /locus_tag="PK610_08005"
     CDS             complement(1984..2694)
                     /locus_tag="PK610_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35917.1"
                     /translation="MNRYEKKAQQDKTLGILNDIEDTASDRKLPMGKRMGKGAGSAAI
                     KILSGIGGGIVGAGFGTWSLIPGFVLSATGEVIGSPELTCFGVGMVASGIIPDTSLNG
                     TEDDKKSKFQKAKDRMKTYGKGIMRKFWIDKLVKKEATKTETKTTENTSITTNAETST
                     TTTTDQSTSGMGEVKYYTHPQAEPDPADLAELEKYEEQIRESANSFAQENQISTSENM
                     SGFGDDDRFLPIEEEKIY"
     gene            complement(2727..4433)
                     /locus_tag="PK610_08010"
     CDS             complement(2727..4433)
                     /locus_tag="PK610_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35918.1"
                     /translation="MEAKTQRIVKSGWQFDHLFPKALFTETTVKKNALVEDTVRFIPE
                     VIAKTRWQVKRYVDQELKYLSLFDACEKLWHFIKDHIRYKKDETGKEQVRSPRRLWGE
                     KTGDCDCFTVFIGTCLSLLSELKNKKGSIMLRITKYGKDHFQHIYPVVITEEGKQIIL
                     DCVVEDFNYEEPYTEKKDFQLDINTLNKMDLNYLDGVPMKENSDTQLMEDNSELGKLF
                     DFLKRKTDPATGQKKPGILQKVGTAVKTAAKKVGTVTKKTLNVINKVNPATVLLRAGV
                     LASMKLNVMKVSQRLKYAYLSDAEAQKRGVDMVKFQKLKQIKDKIEKIFYGAGGDPKN
                     FKEAVLTGKGNEKREVPLSGLGNISSESAIGMNQNTPLTKILGQEMYYSEFMDGTNEI
                     EGLGSLGEPATGTAIAAASGVLASIAALLKNLGNLFPKKNKEAADFENVADANAEADK
                     VNASSDEVDLDKLTEDLENESRQTRSLTTDETTNADNSNGGDPPADGTWWERNKKWLK
                     PTLWGTGIATTLGLGYLALRPKKGAKKKDKSLEGVGSKPKPKKKGRKKAKTQKVKNIT
                     LK"
     gene            complement(4433..4930)
                     /locus_tag="PK610_08015"
     CDS             complement(4433..4930)
                     /locus_tag="PK610_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35919.1"
                     /translation="MGIVKKILIGTGITAAVAGVVTYVSRLNKMSTELVIVPTVKVHK
                     IALDGITLRVDVRMKNPTRTKIRIKFPFVKLIYKNSVVGSSQSVDKDIEVPAFGEAQV
                     DAMMVQIPFLGLFSIAGDLIKSLQDGTAVKVGVKTTTLINLGWKKIPYEDYQEYNLKN
                     ETPTT"
     gene            complement(4917..5120)
                     /locus_tag="PK610_08020"
     CDS             complement(4917..5120)
                     /locus_tag="PK610_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35920.1"
                     /translation="METTKTHNDSTSHMLLTGTILFANLDYNGLLDYAIKAILGGGIW
                     MVFKLGSDYFSGKIRKKQKDGNR"
BASE COUNT         1124 a         1178 c         1177 g         1790 t
ORIGIN      
        1 cgccgacctc taccacggcg aatgataaag tgctttaagc ggatatggta gttgtcgcca
       61 aagccgagtt catgcattct gcgtttaatg tattcctgtg ccaaagccgg tgtcatggtt
      121 tttcttttag ttgttggtag atatacagat caatttcata gcggacgttc cttcccgata
      181 tcactcccca attgtcatgg atgtatccgt gaacgatggc agtatcggca tcgactttta
      241 ctttcaggct ctgcggaaac tttgcgtgtg aataattgtt cacccgaaaa acgccacccg
      301 tataatccag taaaccataa ttggcattgt ccaggattat ttttccgaag taaaaaacac
      361 cttcacgttg gttacttgaa agcgagagtt caccaatgat cacttccggt tggtagggag
      421 agcggctttt gagttccgtt accacaccgc tttctgcatc cgtaatcagg cggactttat
      481 accacagccc ggtgatgaac ttcgcattgt ttgaaagagg gatttcaaaa tgcttgagtt
      541 caccttgtcg gttgatggct accgtatgaa tgatgagtcc ttccattggt attagtcaat
      601 tacgatttca cagtcgaaat acaacagcac ggtataggca gcaaatacgg tacgtccatc
      661 gggcttgtcg gtgtattcta tttttacctt gccgtttccg ataatcacct ttttaagtgg
      721 ccagtaacgg ttatcgagat tagcgttgat gccgttcatg acgcgttgca cggcatggtt
      781 atcgggaaag tattccttgt tgttaatctc caatcgtgcg tacccgcgat ggtacatcag
      841 atcgggtttt tcactggaga aagcaacccc gtgtactttt tttacgcttt tgtccagttc
      901 aaaggtctcc gatacttttt gtccggcagc tgtgatctgt atttcaaaaa ctttgggtac
      961 gctatgcaat tcgttttctt ccattttttt attgttgcgt tggtggtttt aatttttttc
     1021 aggttcggga tttcaggttt gcattcaagc agacctgaaa tccgttcctg tttttcattt
     1081 aggaataggt attcctaaag aactcgccct ttagggcgaa atcgccatta tggcgtggtg
     1141 atcgttccgt ggaggccagc gaagatgtag gtgttcgctg cgatgccgtc cattgtgccc
     1201 agctcgatag tcaactcaat cagtacgtca tcctgaatga gtcgcgggtt ggagagtttg
     1261 taataaccca ttggcacaag gttcagattg gaagtcttga acaggttgtt ggaagtatcc
     1321 ggaacgattt gctttttgtt ggccttgaga gagaactcac cgttggcaat ggccgggaaa
     1381 agttcaatct tgtcgtagtt ggtagcgatg atcttgtcct tggtggcatc ggcagaaact
     1441 ccggccaaca ggatgatacc gctaaccagc atagactggt ttttgggaag tttcgcgttg
     1501 gagatattgc gaagaccgat ttccttgtcg tcctgcgtct cgaacatttt aatggttttc
     1561 gaggaagcgg ctttgatgga atagatcacc gtatctgcca agcgcagttc accttttccc
     1621 aatccatctt tgatgtgctt ttgcagttcg ggaaaatgct tttccatttc tgcacgcgat
     1681 ccttttgacg ggaatggatt gcccactaac ttttgcatgg tcttagcgcg ttgcaccatt
     1741 ggcatggctt tgatggcagc gttgagttct tcgctttcca tcatttcagt acccatcaac
     1801 gcacctgcgt tgttgtactc ttcgattgct gataattgtc ctaacatttt tgttttgttt
     1861 tttgagtttt actttttttc tgttttgatt taggcagttc tttcccggat ggagaagtgc
     1921 gcagcttctc catgtggcga gtaaaatctc gccattagcc gttaaaaaac tggggtggct
     1981 tgcttagtag attttttcct cttctatggg aagaaacctg tcgtcatcac cgaaaccgga
     2041 catattctcc gaggtgctga tttgattttc ctgagcgaaa gaattagccg actcgcggat
     2101 ttgctcttcg tacttttcca attcagccag atctgccggg tcaggttcag cctgtggatg
     2161 cgtgtagtat tttacttcac ccatgccact ggtggattga tccgttgtgg ttgttgtaga
     2221 agtttcagcg ttggtcgtga tactggtatt ctcggtagtt ttggtttccg ttttggttgc
     2281 ttccttctta acgagtttgt ctatccaaaa tttccgcatg attcctttac cgtaggtttt
     2341 cattcggtct ttggcttttt ggaatttgga ttttttatca tcctcggttc cgttgagcga
     2401 agtatcggga atgatgccgg aggcgaccat gcccactccg aagcaggtca gttccggtga
     2461 accgatcact tctccggtcg cgcttaatac aaatcccgga atcagcgacc atgttccaaa
     2521 acctgctccc acaattccac ctccaattcc ggagagaatt ttaatggctg ctgatcctgc
     2581 gccttttccc atgcgtttgc ccattggcag cttgcggtcg gaggcggtat cctcaatatc
     2641 gttgagaatg ccgagggttt tatcctgctg tgctttcttt tcgtacctgt tcatgacttt
     2701 aatttttttt gatggttgtt acgtttttat ttcagggtga tgtttttcac cttttgggtt
     2761 ttggcttttt tcctgccctt tttcttaggt ttcggtttgg agccgacacc ttcgagtgat
     2821 ttatccttct ttttagcccc tttttttggc cgtagagcga ggtatccgag tccgagggta
     2881 gttgctatgc ctgttcccca taaagtcggt ttaagccatt ttttgttgcg ttcccaccat
     2941 gtgccgtctg ctggcggatc gcctccatta gaattatcgg cattggtcgt ctcatcggtg
     3001 gttagtgacc gggtctgcct tgattcgttt tccaaatcct cggtgagttt gtccaagtcc
     3061 acttcatcag aagaagcatt caccttatcg gcttcggcat tggcatcggc cacgttttca
     3121 aaatcggcag cctctttatt cttttttgga aataaattac cgaggttctt cagcaacgct
     3181 gcaatggaag ccagtacacc agatgctgct gcaatggctg tcccggtagc aggttcgccc
     3241 aacgatccaa gtccttcgat ttcgtttgtg ccatccatga actcggagta gtacatttcc
     3301 tgtccgagta ttttggttag cggtgtgttt tgattcatcc cgatagccga ttcgctggag
     3361 atatttccaa gaccgctgag aggaacctca cgcttttcat ttccttttcc ggttaaaaca
     3421 gcctccttaa aatttttggg atcaccgcct gccccgtaga atattttttc aattttgtct
     3481 ttgatctgct tgagcttttg aaactttacc atatccactc cgcgcttttg cgcttcagca
     3541 tcgctcaggt aggcgtactt tagtcgctgc gaaactttca ttacgtttag tttcattgaa
     3601 gccaatactc cggcacgaag caagacggtg gcagggttta ccttgttgat tacgttgagc
     3661 gttttcttgg taacggttcc cactttctta gcagcggttt ttactgccgt tcctactttt
     3721 tgcagtatgc cgggtttttt ctgtccggtg gcggggtcgg ttttcctttt gagaaaatca
     3781 aagagttttc caagttccga gttgtcttcc atgagttgcg tatcggaatt ctctttcatc
     3841 ggtacgccat cgagataatt taaatccatt ttatttagag tattaatgtc caactgaaaa
     3901 tctttttttt cggtgtaagg ttcttcgtag ttaaaatctt ccaccacaca gtccaggatg
     3961 atttgttttc cttcttcggt aatcactacc gggtagatat gctgaaagtg atccttccca
     4021 tacttcgtta tccggagcat aatgctccct tttttatttt ttaattcgct cagtagagag
     4081 aggcatgtgc caatgaaaac agtaaagcaa tcgcagtccc cggttttttc tccccataac
     4141 ctgcgcggtg agcgcacttg ttcttttcct gtttcatctt ttttgtagcg gatatggtct
     4201 ttaatgaagt gccataactt ctcacaggca tcaaaaagcg aaaggtattt tagttcctga
     4261 tccacgtatc gcttcacctg ccaccgcgtt ttggcgatca cttcaggaat gaatcgaacc
     4321 gtatcttcca ccaaggcatt tttcttaaca gttgtttccg taaagagtgc ctttggaaaa
     4381 agatggtcaa actgccatcc gctttttact atgcgctgtg ttttggcttc catcaggtag
     4441 taggtgtttc attttttaag ttgtactcct gataatcttc ataaggaatt tttttccatc
     4501 caaggttaat cagcgtagtg gtttttaccc caaccttgac tgctgttccg tcttgcagag
     4561 atttaatcaa atctcccgct atggaaaaca atccaaggaa aggaatctgt accatcatgg
     4621 catctacctg tgcttctccg aaagccggaa cttcaatatc cttgtccaca gattgcgaag
     4681 accccaccac ggagtttttg taaatgagtt ttacaaatgg gaattttatt ctgattttgg
     4741 tgcgggttgg atttttcatt cgtacatcca cgcgcagggt gattccatcg agagcgatct
     4801 tatgcacttt caccgttggt acaatgacca gttctgttga cattttgttt aacctgctca
     4861 cgtaggtaac cactcccgcc accgctgcgg tgatcccggt tccgattaat atttttttaa
     4921 cgattcccat ctttttgttt tttccggatt ttacctgaga aataatctga gcctaatttg
     4981 aacaccatcc aaatgccccc tccgagaatt gcttttatcg cgtaatccag taatccgtta
     5041 tagtcgaggt tcgcaaaaag gattgttccg gtcaggagca tatggctggt tgaatcatta
     5101 tgtgtttttg ttgtttccat tttttatttt gtgatgcgct ttcttacaat cacggtacaa
     5161 agttgaagca tgggtttgac tcactgttcg gttcatgtag ctaatggtag ctttgggtaa
     5221 tagttttgtc gctttgggta ataactatgt cgctaatggt cgctatcat
//
LOCUS       DAOMYG010000217         5264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5422_length_5264_cov_8.473603, whole genome shotgun sequence.
ACCESSION   DAOMYG010000217 DAOMYG010000000
VERSION     DAOMYG010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5422_length_5264_cov_8.473603"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PK610_08025"
     CDS             complement(<1..457)
                     /locus_tag="PK610_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ35921.1"
                     /translation="MSESTVLIIDDNLSRQAQLEQILDPLPCTVIRTDTSEKALDILR
                     YTEVSVIILDYNLKGMELHSFMDQIRNDPSSEDVHVILTLDELHRKSHIADLYRSGAV
                     DYIERPFQPETIRSMVKVFVRLFQKSKKVKELLLNILPREIASELKEAHK"
     gene            555..1928
                     /gene="hisS"
                     /locus_tag="PK610_08030"
     CDS             555..1928
                     /gene="hisS"
                     /locus_tag="PK610_08030"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015363558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRJ35922.1"
                     /translation="MAEKPSIPKGTRDFGPTEMTRRNYIFETIRRHFRRYGYLPIETP
                     AMENLKTLMGKYGEEGDRLIFRILNSGDFLEGANLQEIQTKGSLFAASQFSEKALRYD
                     LTVPFARFVVQHQNEISFPFKRYQIQPVWRADRPQKGRYREFFQCDADVIGSDSLLNE
                     IELIRLMDDVFSDLTIKTTIKINNRKILSGIAEVTGESDRIVEMTVAIDKLDKIGEEA
                     VLNEMREKNISENAIEKIKPLLALKGSPAQQFAQMRNVLSQSQVGLKGIEELEFIFNY
                     FGTTTHQLKTAILELDITLARGLNYYTGAIFEVKSNEGDLKVSITGGGRYDDLTGIFG
                     LKGMSGVGISFGADRIYDVLSSLERFPEDAGESTRLLFVNFGEQESRWVMDLLPILRN
                     AGINCELYPDKSKMTKQMKYADDKKIPYVALVGSSEMEQGKITVKDMRSGEQNSFGQN
                     ELIEKLK"
     gene            1931..3439
                     /gene="hutH"
                     /locus_tag="PK610_08035"
     CDS             1931..3439
                     /gene="hutH"
                     /locus_tag="PK610_08035"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HRJ35923.1"
                     /translation="MSTFEISPNPLTIKQIDQVLKQQHVLGLSSQSRQRIENCYSYLH
                     TKLKNSDLPIYGINTGFGSLCNTQIDKEDLSQLQENLVKSHACGSGEEVDPEIVRLML
                     LLKIQALALGHSGVSTATVDRLIFLYNEQAYPVVYQLGSLGASGDLAPLAHLSLALLG
                     LGMLNYNGKKISCEDFYKERNIQPLRLEAKEGLALLNGTQFMSAHGVHLMIRMHKLSA
                     MADCTGAVSLEAFFGLDTPFHPSLHKIRPHAGQAITASNFRHILSGSGLQKAKKNQVQ
                     DPYTFRCIPQVHGASKDQFRWIEQIVETEINSVTDNPTIFPEEDLILSGGNFHGQPIA
                     IAFDLLCIAAAELGSISERRIYKLISGQRDLPAFLTPEPGLNSGLMIPQYTAASIVSQ
                     NKQLCMPASVDSIDSSNGQEDHVSMGANAATKALRIVNNLERILSIELLNASQALSFR
                     TEGKSSALIENWMNEFRVKVPIIREDILMHRHMEEALKFIQTEQPITGVQSW"
     gene            3488..>5264
                     /locus_tag="PK610_08040"
     CDS             3488..>5264
                     /locus_tag="PK610_08040"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HRJ35924.1"
                     /translation="MTRILLTLTLLLAAIGSTAQDNKKYQGLLWEISGNGLSKPSYLY
                     GTMHVSEKLAFHLSDTFFIALKNVDLVALELDPGKWMEETLEMDNAFEQKEYVTSARS
                     ASGYYSSAFKPFFPEKNELMRLLRSKPRIINNMLYRSNEAYDEFEEDTYLDLFIYQTG
                     KKLGKNVIGLEDFMVSRKLVDKAYQGEDDEDDENYKNQKERNRILIRQMEKVKSMREY
                     QEDSYRNGDLDMLDSIYRLTNPGTVFLRYMLWERNVIMANQMDSIMKKQTLFTGVGAA
                     HLPGEKGVIELLRSKGYKVRPIVYTNINGDKRRTEIEKMKFPVTFKTRFPEDSAFSCE
                     VPGELFRTLDRPGKIEYLYNDMSNGSYYHIQRLAYLGKLDGKDQQHTLKRLDSLLYEN
                     IPGKIQSKKEIKASNGYSGWDIVNKTRRGDLQRYQILVGPLEIFVFKMSGPNEYISGK
                     EGDQFFNSIKWKSVDLSARSVYSPKAGGFTIMMPAVRVINRAYALRSGILRHAISASD
                     ENGDLYFMFHSQVNDFRYIEEDTFELNYLSEVFAEELKAKITRRSLVGPTELHFSIRK
                     EERNIRLRILIQGENYYMAGTTCTDS"
BASE COUNT         1636 a         1076 c         1158 g         1394 t
ORIGIN      
        1 gtttgtgcgc ctctttcagt tctgatgcaa tttcgcgcgg aagaatattg agcagaagct
       61 cttttacttt ttttgatttc tgaaagaggc gcacaaacac ctttaccatc gatcgaatgg
      121 tctccggctg aaagggacgt tcaatgtaat cgactgcgcc ggaacggtac aaatcggcaa
      181 tatgcgattt gcgatgcaat tcgtccagtg ttaaaatcac atgcacatcc tcgctggaag
      241 gatcatttct gatctgatcc ataaaagaat gcagctccat acccttgaga ttataatcca
      301 gaataattac agaaacctcc gtataacgca gaatatccag ggctttttcg gaggtgtcgg
      361 tgcggattac cgtacagggt aacggatcaa gtatctgctc cagctgggct tgccgggaga
      421 ggttatcatc gatgatcagg actgtagact cggacataga gttccgaaaa atagctaaaa
      481 aagagatatg cgattcaggc gaattatccc acttcattgc gctgaatgtg tatttttgat
      541 gtccacaacg cattatggca gaaaaaccat ccatccccaa aggcacacgc gattttggtc
      601 caaccgaaat gacgcgaaga aattacattt tcgagaccat ccgcaggcat ttccgacgtt
      661 atggctattt gcctattgaa actccggcga tggagaatct gaaaaccttg atgggaaaat
      721 acggtgaaga aggtgatcgc ttgatttttc gcattcttaa cagtggtgat tttttagaag
      781 gagccaactt acaggagatt caaacgaaag gttccttatt tgccgcttct caattcagtg
      841 aaaaagccct ccgctacgat cttactgtac cctttgcacg atttgttgtt cagcaccaga
      901 atgaaatcag ttttccattt aaacgctatc agatccaacc tgtatggcga gctgatcgtc
      961 cgcaaaaagg ccgttaccgc gagttcttcc aatgcgatgc agatgttatc ggatcagatt
     1021 ccttactcaa tgaaatcgaa ttgattcgcc tgatggacga tgtgttcagt gatctgacta
     1081 ttaaaaccac cataaaaatc aataaccgca aaatcctttc cggaatagca gaagtcaccg
     1141 gcgaatccga ccgcattgtg gaaatgacag ttgccattga taaactcgat aaaataggtg
     1201 aagaagcggt tttaaatgag atgcgcgaaa aaaacatcag tgaaaatgcc attgaaaaaa
     1261 taaaaccact cctcgcactt aagggaagtc ccgctcaaca atttgcacaa atgcgaaatg
     1321 tactgtctca atcgcaagtg ggattaaagg gaattgaaga gctggaattt attttcaact
     1381 atttcggcac cacaactcat cagctgaaaa ccgcaattct tgaactggac atcacgctgg
     1441 caagaggatt gaattattat accggtgcca ttttcgaagt aaaatcgaat gaaggagatt
     1501 taaaagtaag cattacaggt ggaggaagat acgatgatct caccggaata ttcggtttaa
     1561 agggaatgtc gggagtaggt atttcatttg gggctgacag aatctacgat gtactttcca
     1621 gtctggaacg cttccctgag gatgcaggag aatcgacgcg cttgttgttt gtcaattttg
     1681 gcgagcagga atcgcgttgg gtaatggatc tgctacctat acttcgaaat gcaggaatta
     1741 attgcgaact ctatcccgat aaatcgaaaa tgactaaaca aatgaaatat gccgatgata
     1801 aaaagatccc ctatgtggca ttggttggat catcggaaat ggaacaggga aaaattaccg
     1861 ttaaagacat gcgttccggc gaacaaaaca gctttgggca gaatgagctc atcgaaaaac
     1921 taaaatgatc atgagcacat tcgaaatcag tcccaacccg cttacgataa aacaaattga
     1981 ccaggtgttg aaacaacaac atgtccttgg tctttcatct cagtccaggc aacgcatcga
     2041 gaattgttat tcgtacctgc atacaaaact gaaaaattct gatttaccta tatacggaat
     2101 caacaccgga tttgggtcat tgtgcaatac gcaaatcgac aaagaagatc tttcgcagct
     2161 acaagaaaat ctagtgaaat cgcacgcctg tggttcaggt gaagaagttg acccggaaat
     2221 tgtccggctc atgttattac tgaaaattca agcacttgcg ctcggccatt caggtgtatc
     2281 aacggctaca gttgatcgtc tgatttttct ttacaatgaa caagcctatc cggtcgttta
     2341 ccaattggga tcgcttggtg cttcgggcga ccttgctccg cttgcacatt tatcgcttgc
     2401 acttcttgga ttgggaatgc tgaattacaa tgggaaaaaa atcagctgcg aagactttta
     2461 caaagaaaga aatattcagc cactccgact tgaagcaaag gaaggattgg ctttgctcaa
     2521 cggaacgcaa ttcatgagcg cacatggtgt acatctcatg attcgcatgc acaaactcag
     2581 cgcaatggca gattgcacag gagcggtttc ccttgaagct ttttttggtc ttgatactcc
     2641 tttccatccg agcctgcata aaatccgtcc gcatgccggt caggctatta cagcttccaa
     2701 tttcaggcat atcctctcgg gtagtggatt acaaaaagca aaaaagaacc aggtacagga
     2761 tccatatacc ttcaggtgta ttccacaggt tcatggagca agcaaagatc agttccgctg
     2821 gatcgaacaa attgtagaaa cagaaattaa ttcggttacc gataacccaa ctattttccc
     2881 ggaagaggat ttgattttat caggagggaa tttccacgga caacctatcg caattgcatt
     2941 tgatctattg tgcatcgccg ctgctgaatt gggaagtatt tcagagcgaa gaatttataa
     3001 actcatctcc ggtcaacgcg atctccccgc atttcttact cccgaaccgg gactgaattc
     3061 cggattaatg attcctcagt atacagcagc ctccattgtc agtcagaata aacaattgtg
     3121 tatgcctgcc tcggtggata gtattgactc aagcaatggg caggaagacc acgtaagtat
     3181 gggagctaat gcagcgacca aagcccttcg cattgtcaat aatcttgaac gcattttatc
     3241 gattgaattg ttgaatgcat cccaggctct aagtttcaga acagaaggga aaagttcagc
     3301 attgattgaa aactggatga atgaattccg agtgaaggtt ccaatcattc gggaagatat
     3361 tttgatgcac cgtcacatgg aagaggcgct taaatttatt caaactgagc agcccataac
     3421 tggtgtgcaa agctggtaag gcaattttac tgttactttt tcgttggttt ttattatacc
     3481 cattactatg acacgaatat tactcacact tacattgctt ttagctgcta ttggctcaac
     3541 ggcacaggac aacaaaaaat accagggatt actttgggaa atttcaggta atggtttaag
     3601 caagccttcg tacttgtacg gaaccatgca cgtaagtgaa aagctggcct tccacctgag
     3661 tgatactttt ttcattgcac tcaagaatgt agatctcgtt gccctggagc ttgatcccgg
     3721 aaaatggatg gaagagacat tggagatgga caacgccttt gaacagaaag agtatgtaac
     3781 ctctgcccga tccgcttccg gatattattc ttctgcgttc aaaccattct ttcccgagaa
     3841 gaatgagctt atgcgactac tgcgaagtaa gccaaggatt atcaacaaca tgttgtaccg
     3901 ctccaatgag gcatacgatg aatttgaaga agatacatat ctggatctct ttatctatca
     3961 gacaggaaaa aaattgggga aaaatgtaat tggactcgaa gattttatgg tctccagaaa
     4021 attagtggat aaagcatatc agggagaaga cgatgaagat gatgaaaact ataaaaacca
     4081 aaaggaaaga aacagaattc tgattcgcca gatggaaaag gttaaatcca tgcgcgaata
     4141 ccaggaagac tcataccgca atggcgattt agatatgctg gattccattt acagactcac
     4201 taatccggga actgtttttc ttcgctatat gctatgggaa cgcaatgtta ttatggcgaa
     4261 tcagatggat tccatcatga aaaaacaaac gctatttacc ggtgttggtg cagcccactt
     4321 accgggagaa aaaggtgtaa ttgaattatt acgtagtaag ggatacaagg tcagacccat
     4381 tgtgtatacc aatataaatg gagataagcg caggaccgaa atcgagaaaa tgaagttccc
     4441 tgtaacattc aaaacccggt tcccggagga ttctgccttt tcgtgtgagg tgcccggtga
     4501 attattcagg acacttgatc gccccggaaa aattgaatac ctctacaacg atatgtcaaa
     4561 cgggagttat tatcacattc aacgcttagc ctatttgggt aaattggatg gaaaggatca
     4621 gcaacataca ctgaaacgat tggatagttt gctctacgaa aatatccccg gaaaaatcca
     4681 aagcaagaaa gagatcaagg cgtccaacgg atattcaggt tgggatatcg tgaataaaac
     4741 cagaagaggt gatttgcagc gctaccagat tttggttggc cctctcgaaa ttttcgtttt
     4801 taagatgagt ggtccaaacg aatacatcag cggaaaagaa ggtgatcagt ttttcaacag
     4861 catcaaatgg aaaagcgtcg atctttctgc aagaagtgtt tatagtccta aagcaggagg
     4921 attcacaatt atgatgcctg cagtacgagt gattaaccgg gcttatgcac ttcgatcagg
     4981 aatattacgc catgccatta gcgcaagtga tgagaatggc gacttgtatt tcatgttcca
     5041 ttctcaggta aatgatttcc gatacattga agaagatact tttgaactca attatttatc
     5101 ggaagtattt gcagaggagc tgaaagcaaa aattacccga cgctcattgg tgggtcctac
     5161 agaacttcat ttcagcataa gaaaagaaga gcgaaatatc cgcttacgta tattaattca
     5221 gggtgaaaat tattacatgg ctggaactac ctgtactgat tcac
//
LOCUS       DAOMYG010000218         5233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5460_length_5233_cov_9.696408, whole genome shotgun sequence.
ACCESSION   DAOMYG010000218 DAOMYG010000000
VERSION     DAOMYG010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5460_length_5233_cov_9.696408"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PK610_08045"
     CDS             <1..427
                     /locus_tag="PK610_08045"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRJ35925.1"
                     /translation="TKPKQSYFDVTTIKLDRRFNVHATFNYAINDEIEIVPSVLAQFQ
                     GTYQEILIGSQVRYILVNEKGVYRAVRAGLFYRAKDAGYLSVGIDYDDWVAGISYDIN
                     ISDLNPASRYRGGIEFAVIRILNVYKPKMIQRRICPNYI"
     gene            429..2681
                     /locus_tag="PK610_08050"
     CDS             429..2681
                     /locus_tag="PK610_08050"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRJ35926.1"
                     /translation="MRILLLTLCLCVTFSAGAQTYKQYVKFADKNWDAGDYYSASLYY
                     KKALDIDSLDINVLWRYAECLRLYNDYEKAEAAYSKVHYREETKIFPLSLYYLASMQK
                     YNGKYKEAQKNFKMVNKQFSRNKKSFEYLKSKQEMQSCSFAMKANKKDTSGFKIYNAG
                     EKVNTTDSEFGSSWVDSVLYYASLRADNVKGDLEIHDPHYKIKVYKNPRQGSVFSTSQ
                     ALDTTINSILSHNANGCYSSDGSRFYFTRCIGTECTIMYSEIRNGKYGTPKEANGGVN
                     KEGYTSTQPMIAKIGERDILFFVSNMPNGEGKLDIWSSEITAKGDHTKPRNAGKTVNS
                     IDDEITPFYDSEEGALYFSSTWHDGFGGFDIFRSNGTPEKFGASQNLGFPLNTQWNDM
                     YYSVYPSEYVGFLTSNRKGSLFVKSPTCCNDIYLVEFPRKEIPQDTIPYKSLEDLNKY
                     LPVTLYFHNDEPNPRSNDTTTKLNYMTTYNAYTAMIDKYKEEYSKGLAADKAIKAQDD
                     IENFFIDYVDKGVSDLAIFTRLLLAELEKGQKIELSIKGFASPLAKTDYNVHLTKRRI
                     QSLINFLREYEGGVFIPYLEGTAPNGGQLFFVKIPFGEYSASNVVSDNFHDTRNSVYS
                     RSAALERKIEVQTVQRANKDSIQGEMRVNKESHDFGAAKSGSTLKTTFTVKNTGKGEL
                     KIENIMIGCDCISVEYTREPIPPGGSAEIVVTFNTTGYTGKNVRSVTLVTNGFPANKR
                     LVVTAELMSQ"
     gene            2678..4258
                     /locus_tag="PK610_08055"
     CDS             2678..4258
                     /locus_tag="PK610_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35927.1"
                     /translation="MKNGIVLATKNNRTHHYLHCAMRTLLLLFALHTTPLFLVAQPLT
                     TPEYSRGRIISSTQSDLNLCSISEWKNYKLVVRSNYKGDATRYTVEVFDHENNRAEFS
                     THKFTIPESDRLSFVEVVSLGDVTYLLVSKFSASEKATILFAYPVTEKGILEDERKEI
                     DRLPAYNAGNTGRFRVNVSPDHQFLVVLRENIYDKAKNETIALHRYDTDLNQVWTKTH
                     AFSILSKNQTVNIPFVNNKGDLYLIKKDRDKETFKYYILAAEAKGTIISEKPLQLGNI
                     FISDIRAALLQNGNLGVAGFYSTQGYDSYEGSFYFAFDSKTGVAVKHNESLNSEILTL
                     FMAKKDASKSGTALFGFNLHHLIAQENGGMYLIAEKTQQVTEKTAERYTYEDLLILNY
                     DEQGNIKWGKSIRKKQETWGDDGKWSSYNYFLYNDTLNILFNKVIFDDQLTKTGKKKK
                     ADEFGESTGYGTNWARVFPNGQIQITPLTGMHQGTVPLTLNPEILYSDTTGKVFVVTE
                     GHLKQHHLLLGMKFYNRK"
     gene            complement(4285..5190)
                     /gene="rluF"
                     /locus_tag="PK610_08060"
     CDS             complement(4285..5190)
                     /gene="rluF"
                     /locus_tag="PK610_08060"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018944273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HRJ35928.1"
                     /translation="MVNLFSMQSGGIRLNKYISESGLCSRREADRFIEQGNVFINGRR
                     AKIGDQVKAGDRVRVNGHELEPREKENLVFIALNKPVGVTSTTEEGVRDNIVQFVNHT
                     QRIFPIGRLDKDSQGLIFLTNNGDLVNKILRAGNQHEKEYVVTVNKPLTDAVIQGMAN
                     GVPMLGVTTKKCKIVQEAATVFRIVLVQGLNRQIRRMCEHFGYDVIKLERVRIMNIPL
                     KGLAPGDWRELTSQEMESLMSMIKDSSSETQVKPKQNQPGKKKPNTGNSGKAPFKGKR
                     NNSQGSHFGERRSKNSGKQNGKKRR"
BASE COUNT         1678 a         1127 c         1001 g         1427 t
ORIGIN      
        1 caccaaaccc aagcaaagtt attttgatgt aaccacaatt aaactcgaca gaagatttaa
       61 tgtgcatgca acattcaact atgccatcaa cgacgaaatt gaaattgtac cgtctgtttt
      121 ggcacaattc caaggcactt accaggaaat tctaattgga tcgcaagtac gctacatcct
      181 tgtaaatgaa aaaggcgttt atcgcgctgt aagagccgga ttattctacc gtgcaaaaga
      241 tgcaggctac cttagtgttg gtattgacta cgatgattgg gttgcaggaa tcagctacga
      301 catcaacatc tccgatctta atcctgcgag tagataccgc ggtggaattg aatttgcggt
      361 tatccgcatt ttgaatgttt acaaaccaaa aatgatccaa cgcaggatct gtccgaacta
      421 catctgatat gcggattcta ctccttacac tctgcttgtg tgtaaccttc tctgcaggag
      481 cgcaaaccta taaacagtac gtaaagttcg ctgataaaaa ttgggatgcc ggcgattact
      541 acagtgcgtc gctttattat aaaaaggcac tggacatcga cagtctcgac atcaacgtat
      601 tgtggcgtta tgcagaatgc ttgcgactgt ataacgatta tgaaaaagca gaagctgcct
      661 attcaaaggt tcattatcgc gaagaaacca aaatctttcc tttaagcctc tactaccttg
      721 ccagcatgca gaaatacaat ggcaaataca aggaagcgca gaaaaatttc aaaatggtaa
      781 acaagcaatt ttcgcgcaat aagaaatcgt ttgaatacct aaaatcaaaa caggagatgc
      841 agtcatgctc ttttgctatg aaagccaata aaaaggacac cagtggcttt aaaatttaca
      901 atgcaggaga aaaggtcaat accaccgatt cggaatttgg aagttcgtgg gttgattccg
      961 tattgtatta tgcatcgctt cgggccgata atgtaaaagg agatctggag attcacgatc
     1021 cccattacaa aattaaagtc tacaaaaatc cccgacaagg ttctgtcttt agtacaagtc
     1081 aggcgcttga cactacaatc aattccatcc tttcgcacaa cgcaaatggc tgttacagtt
     1141 cagatggttc acggttttat tttacccgtt gtattggtac cgaatgcacg atcatgtaca
     1201 gcgaaattcg caatggaaaa tatggaactc caaaagaagc caatggcggt gtaaacaaag
     1261 agggctacac ctcaacgcaa cccatgattg caaaaattgg tgagcgcgat attttgtttt
     1321 tcgttagcaa catgccaaac ggagaaggaa aacttgacat ctggtcgagc gaaatcactg
     1381 caaagggcga ccataccaaa ccgcgcaatg caggaaaaac tgtaaatagt attgacgatg
     1441 aaatcacgcc cttttacgat agtgaagaag gcgctttata tttttcatct acctggcacg
     1501 atggatttgg aggctttgat attttcagat ccaacggaac acctgaaaaa tttggtgcat
     1561 cacaaaacct tggatttcct ttaaacactc aatggaacga catgtactat tccgtttatc
     1621 cttctgaata cgtcggtttc ttaacatcga atcgtaaggg aagcttattt gtaaaaagtc
     1681 caacctgctg caatgacatt tacctggtcg agtttccgcg caaggaaatt ccccaagaca
     1741 caataccata caaatcactg gaagacctga acaaatacct gccagtaaca ttatattttc
     1801 acaacgatga accgaatcca cgcagcaatg acactacaac aaagttgaat tacatgacta
     1861 cgtacaacgc gtacacggcc atgatcgata aatacaagga agaatactca aaaggacttg
     1921 ctgctgacaa agcgattaag gcacaggatg atattgagaa ttttttcatt gattatgttg
     1981 ataagggagt gagtgatctt gcaattttta cccgtttgct attagccgaa ttggaaaaag
     2041 gacaaaagat tgaactttcc ataaaaggat ttgcatcacc acttgcaaaa acagattaca
     2101 atgtgcacct caccaaaaga agaattcaga gtctgatcaa ttttttacgc gaatacgaag
     2161 gcggtgtttt cattccctat ctcgaaggaa cagcaccaaa cggcggtcag ttgttttttg
     2221 taaaaattcc atttggggaa tattcagcga gcaatgttgt gagcgataat tttcacgaca
     2281 ccagaaactc agtttacagc agatctgccg ctttagaacg taaaattgaa gtgcaaactg
     2341 ttcaacgtgc aaacaaagac agcattcagg gcgaaatgag ggttaataaa gaatcgcatg
     2401 atttcggtgc tgcaaaatcc ggctcaacac ttaaaacaac atttacagtt aagaataccg
     2461 gaaaaggaga gctaaaaatt gaaaacatta tgatcggttg cgattgtatt tctgtagaat
     2521 atacccggga acccattcca cccggaggat ccgccgaaat agtggtgact tttaatacca
     2581 ccggatacac cggtaaaaat gtacgatccg taacactggt caccaatggt tttcctgcaa
     2641 acaaaagact ggtagttacc gctgaattga tgtcgcaatg aaaaacggca tcgttcttgc
     2701 aaccaaaaac aacagaacac accattatct ccattgtgct atgagaacac tattactact
     2761 ttttgcactt catacaacac ctctttttct cgttgcccag ccccttacta cacctgaata
     2821 ttccagagga aggatcattt catccacaca atcggattta aacctatgct caatttccga
     2881 atggaaaaac tacaagctgg ttgtccgatc aaattacaaa ggcgatgcta cccgttatac
     2941 tgttgaagtt tttgatcatg aaaacaaccg tgcagaattc agcacccaca aatttacgat
     3001 accggaaagt gatcgtcttt cctttgttga agttgtaagc ctgggagatg tcacctacct
     3061 gctggtttca aaattctctg catctgaaaa agcaactatt ttatttgctt atccggttac
     3121 agaaaaagga attctggaag acgaacgaaa agaaattgac cgattaccgg cttacaacgc
     3181 aggtaatacc ggccggttcc gtgtaaacgt ttcacccgac caccaattct tggttgtatt
     3241 acgggaaaac atttacgaca aggcaaaaaa cgaaaccatt gcattgcacc ggtatgacac
     3301 cgacctcaat caggtttgga ccaagacaca cgccttttcc attctcagca aaaatcaaac
     3361 tgtaaatatt ccatttgtaa acaacaaagg cgatctgtat ctcattaaaa aggatcgtga
     3421 taaagaaacg ttcaagtatt atattcttgc ggcagaagca aagggaacaa ttatcagtga
     3481 aaaaccactt caactaggaa atattttcat ttcagacata cgtgcagcat tgttacaaaa
     3541 cggcaatcta ggtgtagccg gattttattc tactcaggga tatgactcct atgaaggctc
     3601 tttttatttt gctttcgact ctaaaactgg agttgcagtg aaacacaatg aatcattgaa
     3661 tagtgaaatt cttacattat tcatggcaaa aaaggatgca agcaaatcag gcactgcact
     3721 gtttggattt aatttgcacc acctcattgc gcaggaaaac ggaggcatgt acctcattgc
     3781 agaaaaaacg cagcaggtca cagaaaaaac tgcagaaaga tatacttacg aagatcttct
     3841 cattttaaac tacgacgaac agggaaatat caaatggggg aaatccatcc gcaaaaaaca
     3901 agagacctgg ggtgatgatg gaaaatggag tagttacaat tactttttgt acaatgacac
     3961 cttaaacatc ttgtttaaca aagtgatttt cgatgatcag ctaacgaaaa caggaaaaaa
     4021 gaaaaaagca gatgaatttg gtgagtcgac aggctacgga actaattggg cacgtgtatt
     4081 tccaaatggt caaatacaaa tcacaccgtt aacaggaatg caccaaggaa ctgtaccact
     4141 aactctgaat ccggaaatcc tatacagtga cactaccgga aaagtttttg ttgtaacaga
     4201 aggacacctt aaacaacatc atttgttgtt gggtatgaaa ttttacaatc gaaaataaat
     4261 tatgatttga tttgggattg agatttacct cctctttttc ccattctgtt tgccggaatt
     4321 cttcgaacgc cgctcaccga aatgggaacc ttgagaatta tttcttttcc ctttgaacgg
     4381 tgcttttccg gaattccctg tattcggttt tttctttcca ggttggttct gttttggttt
     4441 tacctgagtt tctgaactcg aatctttgat catactcatc aaagattcca tttcctgact
     4501 cgttaactct cgccaatctc cgggagcgag tccttttaat ggaatgttca ttatacgaac
     4561 acgttcaagt ttaatgacat cataaccaaa atgctcacac atgcgacgaa tctgcctgtt
     4621 caatccttgt accagaacga tccgaaacac cgtggccgct tcctgtacga tcttacattt
     4681 ttttgttgtt acgcccagca ttggaacacc gttcgccatt ccttgaatta ctgcatctgt
     4741 gagcggttta ttcaccgtaa ccacatattc cttttcatgc tggtttccgg cgcgcaatat
     4801 tttgttgacc agatcaccgt tgttcgtcag aaaaatgagt ccctgtgaat ccttatccaa
     4861 acgtccgatt ggaaaaatcc gctgagtatg atttacaaac tgaacaatat tatcgcgcac
     4921 tccttcttct gtggtagaag taacaccaac aggtttattc aatgcgataa ataccagatt
     4981 ctccttttcc ctcggctcta attcatgtcc gttaacacga acacggtcac cggcctttac
     5041 ctggtcgcct attttcgctc tgcgtccgtt gatgaataca tttccctgct caatgaaccg
     5101 atctgcctca cgccttgaac acaagccgct ttcactgatg tatttgttta accgtatacc
     5161 accggattgc atagaaaaca gatttaccat ggtgaaaata caaataaacc acagctttta
     5221 atcagcaact gat
//
LOCUS       DAOMYG010000219         5230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5465_length_5230_cov_8.029758, whole genome shotgun sequence.
ACCESSION   DAOMYG010000219 DAOMYG010000000
VERSION     DAOMYG010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5465_length_5230_cov_8.029758"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PK610_08065"
     CDS             <1..457
                     /locus_tag="PK610_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HRJ35929.1"
                     /translation="ENCFVRKQSLLYATNFSVGVNIFFELNKRLAEMMNKHWDYEVEV
                     EETHHTQKLDAPSGTAITIAEGIVENLERKEKWIKGTDEDPAHVNIISHRIDNIPGTH
                     VVRYQSAIDDIEISHVAHNRKGFASGAVLAAEWLVGKKGIFTMRDVLDL"
     gene            469..2187
                     /locus_tag="PK610_08070"
     CDS             469..2187
                     /locus_tag="PK610_08070"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HRJ35930.1"
                     /translation="MEKTIANALLLLLIAVYFASLYKIFEKAGRNKKWEGFVPGYNVF
                     VWIKILNKPWWWILLMLVPGVNFLMLIVLNVELSRSFNLRSFKDDLLMVLFPWYFIYR
                     LAYNPELKFVGPLEFKNPAHKKPMGREWFDALMFAVIAATIIRTLFLEAFKIPSPSME
                     KNLLVGDFLFVSKVSYGAKLPETPISFPFTHNTMPVGNGKSYLEIFTLPHLRLPGFGS
                     VKRNDVVVFNFPAGDSVYTDNTNVTYYQFLNQVAFEQYINRFGDISITADSLLVQHFT
                     PFLKPTVDLLRADLMKQDKIISRPVDKRDNYIKRCVGIPGDVIEVIDRQLFVNGEPSG
                     NTDGMQFNYNINANSPIAAANNGQKFKSFLKEKFDVNSDDCNILDYDRGQFIVPLTNE
                     KLAQMQQIYGAANVRPVVKPKGHYQSIKSLYKIYDEYNYLTEGHNQYLCRDLRYLPIF
                     PNDIRYDWSEDNFGPLKIPACGETVELNLENLPMYARIIDVYEKNDLEIKDGKIYING
                     AESTAYTFKMNYYWLMGDNRQNSADSRFWGFVPEDHVVGKAVLVWFSTDPETGIRWNR
                     VFKGIR"
     gene            2535..3899
                     /locus_tag="PK610_08075"
     CDS             2535..3899
                     /locus_tag="PK610_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013549473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRJ35931.1"
                     /translation="MKVHFIAIGGSAMHNLAIALSRSGDVVTGSDDEIVEPSKGRLQR
                     EGLLPEKMGWDSARVTNNLDAVIVGMHARSDNPELAHARELGLRIYSYPEYVYERTKD
                     KTRIVIGGSHGKTTITSMILHVLNVTGIQHDYLVGAQLEGYDCMVGFSSDSKIAVIEG
                     DEYLSSPEDRRPKFHLYRPHIALISGIAWDHINVFPTFENYAEQFGIFIDLIEPNGAL
                     IYFNGDETLSGLAKTRGKQIEKLPYDAHPAKVKNGVTYLQTDEGKEIAISVFGQHNLQ
                     NLNGARLVLKRAGVNDEQFYHAIPSFRGASRRLELLGEKKNRSIFRDFAHSPSKLKAT
                     VAAVKEQFPERRLIACMELHTFSSLTAAFLNEYAGCMDQADIALVYFNPHTIAHKRLD
                     PISEEQVKKAFAREDLQVYTASSAIIAAIRKFDLGNSSLLLMSSGNFDGVDLPAFAKD
                     LLKN"
     gene            complement(3885..4439)
                     /locus_tag="PK610_08080"
     CDS             complement(3885..4439)
                     /locus_tag="PK610_08080"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HRJ35932.1"
                     /translation="MGPLFRLSLACAAIFAILKIILFQTGKHESLYMLVVFGNILLML
                     VSMAGGLFQLRKQQKKASTYADDVKTTMKSAALYVLFASSFAYLYYTKIDPQSVQERI
                     QVRVTQAEAQDFEELKKAHPDQLKSKTRHDFIEDERKFAAMIFSPFFMSTVMMISGLL
                     LGFFYSLLIAWFWRRFIDKASVFE"
     gene            complement(4439..>5230)
                     /gene="rho"
                     /locus_tag="PK610_08085"
     CDS             complement(4439..>5230)
                     /gene="rho"
                     /locus_tag="PK610_08085"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRJ35933.1"
                     /translation="TMSTRIMDLFAPIGKGQRGMIVAQPKTGKTVLLKEVANAIAENH
                     PEIYLIVLLIDERPEEVTDMARSVKAEVIASTFDEPADRHVKVSNIVLEKAKRLVECG
                     HDVCILLDSITRLARAYNTVQPASGKVLSGGVDANALHKPKRFFGSARKIENGGSLTI
                     LATALTETGSKMDEVIFEEFKGTGNMELQLDRKLSNRRMFPSIDILASGTRREDLLCD
                     KETLQRVWILRKHLADMNSIETMEFIRDRMKDTRNNDEFLVSMNS"
BASE COUNT         1544 a         1042 c         1143 g         1501 t
ORIGIN      
        1 ggaaaattgc tttgtaagaa agcaatcctt gttgtatgcc accaattttt cggtgggagt
       61 aaatatattt tttgagctga ataaacgtct tgctgaaatg atgaacaagc attgggacta
      121 tgaagtggag gtggaagaaa ctcaccatac ccagaaactc gatgctccaa gcggcacagc
      181 aataactatt gccgaaggga ttgtggaaaa tcttgaacga aaagaaaaat ggattaaagg
      241 gacggatgaa gatcctgctc atgttaacat aatttcacac cgcatcgaca atattccggg
      301 cacacatgta gttcggtacc aatctgcaat tgatgatatc gaaatcagtc atgttgcgca
      361 taaccgtaaa ggatttgcat ccggagcagt tttggcggca gaatggttgg ttggaaaaaa
      421 aggcattttc acaatgcgtg atgtcctcga tttgtaatac aatacactat ggaaaagaca
      481 attgctaatg ctcttttatt attattgatc gccgtttatt ttgcgagtct gtacaagatt
      541 ttcgaaaaag caggtcgcaa taaaaaatgg gaaggatttg ttcccggata caatgtattt
      601 gtctggataa aaatactgaa caagccctgg tggtggatac tccttatgct cgtacctgga
      661 gtgaattttc tgatgttgat tgtgctaaac gtagagcttt ccagaagttt caacttacgt
      721 tcgtttaagg atgacctcct catggtgctt tttccctggt attttattta ccgactggca
      781 tacaatcccg aattgaaatt tgttggtccg ctggagttta aaaaccctgc gcataagaaa
      841 cccatgggtc gcgaatggtt tgatgcattg atgttcgctg taatcgcagc tacgatcatc
      901 cgaacacttt tccttgaggc gtttaaaatt ccttcacctt ctatggagaa aaatcttttg
      961 gttggagatt ttctctttgt aagtaaagta agttatggcg ccaaattacc ggagacgcca
     1021 atatccttcc catttacaca caatactatg ccggttggga atggtaaaag ttatttggaa
     1081 attttcaccc ttcctcattt gcgcttaccc ggttttggat cggtaaaacg caatgatgtt
     1141 gttgtattca attttccagc cggtgattct gtgtatacag ataataccaa tgtcacctat
     1201 taccagtttt taaatcaggt tgcctttgag caatacatca atcgttttgg agatatttcc
     1261 ataaccgccg attctctttt ggtgcaacat ttcactcctt tcctgaagcc tacagttgat
     1321 ctattgcgtg cagatctgat gaagcaggat aaaatcattt ctcgtcctgt tgataagcgt
     1381 gataactaca tcaaacgttg cgttggtatt ccgggtgatg tgatcgaggt tatcgaccgc
     1441 caattgttcg tgaatggaga accatccgga aacaccgatg gaatgcaatt caattacaac
     1501 atcaacgcaa acagcccaat tgcagctgcg aacaacggac aaaaattcaa atcctttctg
     1561 aaggagaagt tcgatgtgaa ttccgacgat tgcaacattc tcgattacga tcgtggtcaa
     1621 tttatcgtac ctcttacaaa tgaaaaatta gcccaaatgc agcagattta cggtgctgcc
     1681 aatgttcggc cggttgtgaa accaaaaggt cattaccaga gcatcaagag tctttataag
     1741 atctatgatg aatacaatta cctaaccgaa ggtcacaatc agtatttgtg tcgcgatttg
     1801 cgttatctgc caattttccc gaatgatatc aggtacgatt ggtctgaaga taatttcggt
     1861 ccattaaaga ttcctgcctg tggagaaaca gtggaattaa atcttgagaa tctgccaatg
     1921 tatgcccgaa ttattgatgt gtacgagaag aacgatcttg aaataaaaga cggtaagatt
     1981 tacatcaatg gggcagagag tactgcctat acctttaaaa tgaattatta ctggctcatg
     2041 ggagataacc gacagaattc agccgattcc cgtttttggg ggtttgttcc tgaagatcat
     2101 gttgtaggta aggctgtttt ggtatggttc tcaaccgacc ctgaaacagg aatccgctgg
     2161 aaccgggttt tcaaaggaat acggtaagca aatcattatt cataaaaaaa ggctgtcgga
     2221 aaccgacagc cttttttatt ttgaacctga atcgcagagc ttcaggtata tagtaaagtt
     2281 ggcgggtggt aaaaaaaaag aaaacactac ttgctatgaa actttcacca cccgccggga
     2341 aaagaacgcc agttgagttg attggattaa aaatacgaga gtattacgtt ataaatacta
     2401 tttgagattc atgaaattga aactaaaatt gggtatttat tacacttcta gctttaacac
     2461 aattgtgtgt acaagtatgt aaagcgctaa gaaatcaagg cttaagcctt cttaggttag
     2521 caaaaaattt gtctatgaag gtccatttta ttgccattgg cggaagtgcc atgcataacc
     2581 tggcgatagc cctttccaga agcggagatg tggtaacagg ttctgatgat gagattgttg
     2641 aaccttcaaa aggcagattg cagcgtgaag ggctcttacc tgagaaaatg ggttgggatt
     2701 cggctcgggt cacgaacaat ctggatgcag tcattgttgg aatgcatgcc cgatcagata
     2761 atccggagct ggcgcacgca cgggaacttg gattgcggat ctattcgtat ccggaatatg
     2821 tttacgaacg cacaaaagat aaaacacgaa ttgtaatagg agggagtcac ggtaaaacaa
     2881 caattaccag tatgattctt catgtgctca atgtaaccgg aattcaacac gattacttgg
     2941 ttggggcaca attggaagga tacgattgta tggtcggatt ttcatccgat tcgaaaattg
     3001 cggtgataga aggagatgaa tatttgagtt cgccggaaga tcgccgtcct aaattccatt
     3061 tgtaccgtcc gcacatcgca cttatatccg gaatagcctg ggatcatatc aatgtgtttc
     3121 ccacgttcga aaattatgcg gagcaattcg gaatctttat tgatctgatc gaacccaatg
     3181 gagcactaat ttattttaat ggcgacgaaa cactttcggg tcttgccaaa accagaggga
     3241 aacagattga aaaacttccc tacgatgcac atcccgcaaa ggtgaaaaac ggggtgacct
     3301 atttacaaac tgacgaagga aaagaaattg caataagtgt ttttggtcaa cacaatttac
     3361 aaaacctgaa cggagcacgt cttgttctga aaagagcagg tgttaatgat gaacaatttt
     3421 atcatgccat tccttcattc agaggggcat cacgccggct tgaattgctt ggggagaaaa
     3481 agaacagaag tattttccgt gatttcgcgc attcaccatc caaattgaaa gcaacagttg
     3541 ccgcagtgaa agagcaattt ccggagagaa gattaattgc ctgtatggaa ttgcatacgt
     3601 tttcgagttt aacggccgct tttctaaatg agtatgcagg atgtatggat caggcggata
     3661 ttgcgctggt gtattttaat ccgcacacca ttgcgcataa acgactggat ccgataagtg
     3721 aagagcaagt aaaaaaagca tttgcccgtg aggatcttca ggtgtatact gcttcttcag
     3781 ccatcatagc agcaatacga aaatttgatc tgggaaacag tagtttatta ctgatgagct
     3841 cgggaaattt cgacggtgtt gaccttcctg cttttgcgaa agacttactc aaaaactgaa
     3901 gccttatcga taaaacgtcg ccagaaccag gcaatgagca gtgaatagaa aaagcccaat
     3961 aacaaaccac tgatcatcat tactgtactc ataaaaaacg gcgagaagat catcgctgca
     4021 aattttcgtt catcttcaat gaagtcgtga cgtgttttcg attttagttg atcaggatgt
     4081 gcttttttca actcttcaaa atcctgtgct tctgcctgtg taacgcgaac ctgaattctc
     4141 tcctgtaccg attgcggatc gattttggta taatagagat aagcaaatga gctggcaaac
     4201 aatacgtaca atgcagccga tttcattgtt gttttcacat catcggcata ggtgctcgct
     4261 ttcttctgct gctttctcaa ttggaacaat cctccggcca tactcaccag catcagcaga
     4321 atatttccaa atacaacaag catatataac gactcatgtt ttcccgtctg aaaaaggatg
     4381 attttcagta tcgcaaaaat ggccgcacag gcaagtgata atcggaaaag tggtcccatc
     4441 agctgttcat cgaaacgagg aattcatcgt tattccgggt gtctttcatt cggtcgcgga
     4501 tgaactccat agtttcaatc gaattcatat ctgccaggtg cttgcgaagg atccaaacgc
     4561 gctgaagggt ttccttatcg cacaacagat cttctcttcg tgtacctgac gcaagaatat
     4621 cgatggatgg aaacatacga cgattcgata atttacggtc gagctgaagt tccatattac
     4681 cggttccttt aaattcttcg aaaatgactt catccatttt agaaccggtc tcagtaagtg
     4741 cagttgcaag aatggtcagc gaaccgccat tttctatttt tcgtgccgaa ccaaagaaac
     4801 gttttggttt gtgcagcgca ttggcatcca caccaccgga taatacttta ccggatgcgg
     4861 gctgaaccgt attataagca cgggcaagac gagtgatgga gtcgaggagg atacaaacat
     4921 catgaccaca ttctaccaga cgttttgctt tttcaagaac aatattgctc actttcacgt
     4981 ggcggtcagc tggttcgtcg aatgtagatg caatgacttc tgcctttaca ctgcgtgcca
     5041 tgtcggttac ttcttccgga cgttcatcaa tcaatagtac gatcagatag atttcaggat
     5101 gattttctgc aatggcattg gctacttctt tcaacaatac cgttttaccg gttttcggtt
     5161 gtgctacaat catcccacgc tgacctttac ctattggtgc aaaaagatcc atgattcgtg
     5221 tggacattgt
//
LOCUS       DAOMYG010000220         5220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5474_length_5220_cov_7.816844, whole genome shotgun sequence.
ACCESSION   DAOMYG010000220 DAOMYG010000000
VERSION     DAOMYG010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5474_length_5220_cov_7.816844"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..2000
                     /locus_tag="PK610_08090"
     CDS             171..2000
                     /locus_tag="PK610_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ35934.1"
                     /translation="MPNFKACEEQLKKAQKLDPNFIEAYILLSQVYMDSGKSRDAIEQ
                     LKKSVEINPNFFPNSFYFLAQLELSEGLYEDAKAHAERYLAMPRLPEEMKAKATRLRD
                     NCDFAMKLKKNPVPFKPVNLGSGVNTERPEYFPTITADDQTLLFTRLVVDPKAVQGGI
                     QEDFFVSQKKDGQWIKAKSISARINTLHNEGAPTFSADGQMLIFTACEILDSYGEYRD
                     GFGSCDLFITRRIGEEWSVPVNLGNTVNTGNWESQPSFSADGKTLYFIRGTRSRDGRR
                     TGDIWWTSINPDGTWARPQRLSDVVNTDGNEASVCIHPDGQTLYFSSDGHPGMGGLDI
                     FVTRKKPDGGWSIPENLGYPINTHGEENSLLVSSDGKLAFFASDREGGFGDLDLYSFE
                     LPSSAQPQVTTYMKGIVYDVRTKKPLAARFELIDLKTGEVMVVSQSNGATGEFIVAIA
                     ANRDYALNVNRKAYHFYSKNFSLTEKKGNSDPYLMDIPLVPIDEPEVVTRLDNVFFDL
                     DKATLRPESRIELDKLYSFLQNNPNLKIQLNGHTDNRGDKMRNQKLSEDRAKSVVEYL
                     IAKGIDKSRLSWKGFGDTKPIVPNAQTEAEHQQNRRTEYQILN"
     gene            2003..2761
                     /gene="truA"
                     /locus_tag="PK610_08095"
     CDS             2003..2761
                     /gene="truA"
                     /locus_tag="PK610_08095"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRJ35935.1"
                     /translation="MPRFFLEIAYDGTLYHGWQLQPGVDTVQKEIEAALTKLNSNERV
                     PTLGCGRTDTGVHARQFFLHFDMAGLPGGEELFVFKLNRMLSPSISVYGVTQVGDHAN
                     ARFDATSRTYEYHIHQHKLPFLHNRSWNLWHTLDVDKMNTAAALLLSHRDFASFQKTG
                     GGAKTSICHVMKAQWEQNGDRLVFTIQADRFLRNMVRAIVGTLVQVGLGRLSVEEFQA
                     IIFAGERSEASDSAPGCGLYLTKVTYPYLRETER"
     gene            2758..4563
                     /locus_tag="PK610_08100"
     CDS             2758..4563
                     /locus_tag="PK610_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013188443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ35936.1"
                     /translation="MSKAGGKAFDFGVLKRVLRYVRPYRSLFVGSIFLTVLLAIIGIV
                     RTELLGYLIDFTTGNHTVRPFPGLVDLFEGLTQGKTPVVALNVVTLVIVFILIAEGFF
                     QYFQMLMANRVAQSVTIDLRTSLFSHLLRFRLKFFDTTPIGTLVTRVISDIETIADIF
                     YEGLISVAGDLVKLFVVIFIMFWVNWELALYTLIPVPLLIITTRIFQRSIKKSFMGVR
                     NQVARLNAFVQEHVTGMSIVQIFNREEVEMDKFKSINKEHRKAHIDSVMAYSIFFPVV
                     EILAALSIAFLVWIGTGLALEGRLTFGDIVKYILYVNMLYRPIRMLADRFNTLQMGVV
                     GSERVFSLLDSDHSIADKGTIEAGHFKGEVSMKDVWFAYNDEEYVLKGIDLEVKEGQT
                     IAFVGATGAGKSSLINLLSRLYEFQKGEIRIDGRDIRDYKLMSLRKNIAVVLQDVFLF
                     SDTIHNNISLRDPKISREEIIEASRRVGAHDFIMNLPGNYDYNVRERGSTLSVGQRQL
                     ISFIRAYVTNPSILILDEATSSVDTDSELLIQNAIEKLTEGRTSIVIAHRLSTIQQAD
                     LIVVMDHGKIIEVGSHQDLLAKNGQYKHLFELQFS"
     gene            complement(4830..5141)
                     /gene="ssb"
                     /locus_tag="PK610_08105"
     CDS             complement(4830..5141)
                     /gene="ssb"
                     /locus_tag="PK610_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRJ35937.1"
                     /translation="MNPEVRTLSSGKVMARFSLATTDTYKNEKGERLTETQWHNVIAW
                     GSPARVIEKYVKKGQEIAVEGKLTSRSYEDKSGIKRTVSEVVLSDVLLLGAKDGVKEK
                     N"
BASE COUNT         1462 a         1033 c         1176 g         1549 t
ORIGIN      
        1 ggtagctgca cgttatatta ccggtaactc agccggggta aacagcattt tcaggatttt
       61 cctgcgccag cgctcaaccc aaaagggaat acacaactgc caacaaaaag gcaattaaag
      121 catacgagga aggattaaaa gcctacaata ctttcgatcc gcgtacagga atgccgaatt
      181 ttaaagcatg cgaagaacaa cttaaaaaag cacaaaaact tgatccgaat ttcattgaag
      241 cctatatact cctttcgcag gtgtatatgg attccggaaa aagccgtgat gcaattgaac
      301 aactcaagaa atcggtggag atcaatccga atttctttcc gaacagtttt tattttctag
      361 cacagcttga gctgagtgaa ggattgtacg aagacgcaaa agcacatgcc gagcgttacc
      421 ttgccatgcc acgtttgccg gaagaaatga aagcgaaagc aacccgactg agggataatt
      481 gcgattttgc catgaaactg aaaaagaatc ctgtgccatt taaaccggtg aaccttggtt
      541 caggtgttaa tacagaacga ccagaatatt ttcctacgat caccgcagat gatcagacgt
      601 tattgtttac gcgtttggtg gtggatccaa aggctgttca agggggaatt caggaagatt
      661 tttttgtgag tcagaaaaag gatggacaat ggataaaagc aaaaagtatt tctgcccgca
      721 ttaatacctt acacaatgaa ggtgcaccaa cattttctgc tgatggtcag atgctgattt
      781 ttacagcttg cgaaattctc gattcctatg gcgaataccg tgatggtttc gggagttgtg
      841 atttatttat tacccgaaga atcggagaag aatggtctgt tcccgttaat ctcggtaata
      901 ccgtaaatac cggtaactgg gagtcgcaac cttcgttttc cgcagatgga aaaactttgt
      961 attttattcg tggtacaaga agtcgcgacg gacgaagaac aggagatatc tggtggacat
     1021 ccatcaatcc ggatggtacc tgggctcgtc cccaacgctt gtcggatgtg gtaaacactg
     1081 acggaaatga ggcgagtgtt tgtatacatc ccgatggaca aacattatat ttttcttctg
     1141 acggtcatcc gggcatgggt ggacttgata tttttgtaac gcgtaaaaag cctgatgggg
     1201 gttggagtat tccggagaac ctgggttatc cgatcaatac ccatggtgaa gaaaattcct
     1261 tgctcgtttc ttctgatgga aagcttgcat tttttgcttc cgatcgcgaa ggtgggtttg
     1321 gtgatctgga tctatacagt ttcgaacttc cttcaagtgc acaaccacag gtaacaacat
     1381 acatgaaggg aattgtttac gatgttcgca ctaaaaagcc attggcagca cgatttgaat
     1441 tgatcgatct taaaaccgga gaagtaatgg ttgtgagtca gtccaatggt gcaacaggag
     1501 aattcattgt agctattgct gctaatcgcg attatgcatt gaacgtaaac aggaaagcgt
     1561 accatttcta ttcgaaaaat tttagtctca ctgagaaaaa gggaaattca gatccttacc
     1621 tgatggatat tccacttgtg cctatcgacg agccggaggt tgtgacacgg ctggataatg
     1681 tgttttttga tctcgacaaa gcaacacttc gtcctgaatc gcgaattgaa ttggataagt
     1741 tgtattcgtt tttacaaaat aatccgaatc tgaaaattca gttgaatgga cataccgata
     1801 accgcggtga taagatgagg aaccaaaagc tttctgagga tcgtgcaaaa tcggttgttg
     1861 aatacctcat tgctaaagga atcgacaaat cccgcttgag ttggaaagga ttcggagata
     1921 caaaaccgat tgtgcccaac gcccaaacgg aggcagaaca ccagcaaaat cgtcgtaccg
     1981 aataccagat tttaaattga taatgccccg tttttttctg gagattgcat acgatggtac
     2041 gctctatcat ggctggcaat tgcagcccgg agtggacacc gtgcaaaaag aaattgaggc
     2101 tgcattaaca aagctgaatt ccaatgagcg ggtgccaaca ttgggatgcg gtcgcacaga
     2161 taccggtgtt catgcacgac aattttttct gcatttcgat atggctggtt tacccggagg
     2221 tgaagaatta tttgttttca aactcaatag aatgttgtcg ccttccattt cagtgtatgg
     2281 tgtaacacaa gtgggagatc atgccaatgc acgttttgat gccacatcgc gtacctatga
     2341 gtaccatatc catcaacata aacttccgtt ccttcacaat cgcagctgga atctctggca
     2401 tactttagat gtcgataaaa tgaacacagc tgcggcattg ttgttatccc atcgtgattt
     2461 tgccagtttc cagaaaacag gaggaggggc aaagacctca atttgtcacg ttatgaaagc
     2521 acaatgggaa caaaatggcg atcgactggt atttaccatt caggctgatc gctttctgcg
     2581 taatatggtc cgtgctattg tggggacact tgtgcaggtg ggattgggac gtttatctgt
     2641 tgaagaattt caggccatta tttttgcagg agaacgttcc gaggccagcg attctgcacc
     2701 gggatgcgga ttgtatctta ccaaagtgac ttatccttac cttcgcgaaa cagaacgatg
     2761 agcaaagcag gaggaaaggc atttgatttt ggcgtactta aacgagtgct tcgctatgtc
     2821 cgtccttacc gcagcttatt tgtaggttcc atttttctta cggttctgct tgcaattatc
     2881 ggaatagtac gtacagaatt actcggttac ctgatcgatt ttaccacagg taatcacacc
     2941 gttcgaccat ttcccggact tgttgattta tttgaaggat tgactcaagg aaaaactccg
     3001 gttgttgcgc tgaatgttgt tacactggtt atcgttttca ttctcatcgc agaaggattt
     3061 tttcagtatt tccagatgtt aatggccaat agagtggcgc aatcggttac catcgattta
     3121 cgcacatcgt tattttcaca tttgctccga ttcagattga aattctttga taccacccca
     3181 atcggaacac tggtaacacg tgtgatttcg gacatagaaa ccatagctga tatattttat
     3241 gaaggactaa tttccgttgc gggtgatctg gtgaaattgt ttgttgtaat ttttatcatg
     3301 ttctgggtga actgggagct tgccttatac acgcttattc cggttccctt attgatcatt
     3361 actacgcgga tatttcagag aagcatcaag aaatcattta tgggggtgcg taatcaggtt
     3421 gcgcgattaa atgcctttgt gcaggaacat gtaaccggga tgagcattgt tcagattttc
     3481 aacagggaag aagtggagat ggacaagttc aaatccatta acaaagaaca tcgaaaagca
     3541 catatcgatt cggtaatggc atattcgata ttttttccgg tagttgaaat tcttgctgca
     3601 ctttccatag cgtttctggt atggattggt accggtctgg cgcttgaagg aagattaacg
     3661 tttggagata tcgttaaata cattttgtat gtgaatatgc tttatcgtcc cattcgcatg
     3721 cttgccgatc gtttcaacac cttacaaatg ggagttgtag gttcagagcg tgtattcagt
     3781 cttttggata gcgatcactc cattgccgat aagggtacaa tagaggcagg tcattttaag
     3841 ggtgaagtaa gcatgaaaga tgtttggttt gcttataacg acgaagagta tgtgttaaag
     3901 ggtatcgatc ttgaagtgaa agaaggacaa accattgcat ttgttggcgc cacaggagca
     3961 ggaaaatctt cattgatcaa tttgctcagt cgtttgtatg aatttcaaaa aggagaaatc
     4021 agaatagatg gacgggatat ccgtgattat aaactgatgt cgcttcgcaa aaacattgct
     4081 gtggtattac aggatgtttt tcttttttca gacacgattc ataacaatat cagtttacgc
     4141 gatcctaaga tctcacgcga agagattatc gaggcatcca gaagagttgg ggcacatgat
     4201 tttattatga atcttccggg caactacgac tataacgttc gcgaaagagg aagtacttta
     4261 tcggttggtc aacgccagtt gatttcattc atacgtgcct atgttaccaa tccaagtatt
     4321 ctgattctcg atgaagcaac atcctctgtt gatacggatt cggaattgtt gatacagaat
     4381 gcaattgaaa aactgaccga aggacgcacc tccattgtta ttgcacatcg actttccacc
     4441 attcagcaag ccgatttaat tgtggtaatg gatcatggta aaattattga agtcggcagt
     4501 catcaggacc ttctggcaaa gaatgggcag tacaaacatc tttttgaatt gcaattttcg
     4561 taggatacca ttttttgtcg gatcgcttaa agcgagtaat ttgatttttg atagttgttt
     4621 ggtgaatgga aatggttatt ggttatcagt tattggttat tagccctgaa gcagaaatct
     4681 cccagctgcc gatttcgctt caggatcgat ttttgtttta ggtaccattc aacttcttac
     4741 ttttcactat tcactattca ccgtttacct tacacaaaaa aaatcccctc ttcgctattc
     4801 gcaaagaagg gattttcagg ttggacgggt cagttctttt ccttgactcc gtcttttgcg
     4861 ccaagaagca gtacgtccga caataccact tcggatactg tacgtttaat gccacttttg
     4921 tcctcgtacg atcttgaggt cagttttcct tcaacggcga tttcctgtcc ttttttgaca
     4981 tacttttcga taacccgtgc aggactgccc catgcaatga cattatgcca ttgcgtttct
     5041 gttaaacgtt cacctttttc gtttttgtag gtgtctgttg tggccagtga aaaacgggcc
     5101 atcactttcc cactggaaag cgtacggact tcgggattca tacccaggcg tccaatcaga
     5161 tttacttttg tacctacccc ataattccaa cagcataaaa atataaattc tttttcattt
//
LOCUS       DAOMYG010000221         5208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5495_length_5208_cov_7.973996, whole genome shotgun sequence.
ACCESSION   DAOMYG010000221 DAOMYG010000000
VERSION     DAOMYG010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5495_length_5208_cov_7.973996"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1859
                     /gene="pheT"
                     /locus_tag="PK610_08110"
     CDS             <1..1859
                     /gene="pheT"
                     /locus_tag="PK610_08110"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRJ35938.1"
                     /translation="GDKAELEFPHQKPLDLKKGIFPVKVRVEDQESCKRYAGITIGNI
                     KVAPSPQWLQNRLKAIGARPINNVVDITNFVMHELGQPLHAFDAAAIGGNTIVVRKAK
                     SGKRFITLDDVERELRDVDTMICDEEKELCIAGVFGGSKSGVNDSTQRIFLESAWFDP
                     AHVRKTARGHGLNTDSSFRFERGADPDMVVPALHRAAELIVELCGGVVESDISDFYPV
                     PFSPFEVDIDPEQINKLIGTTISPDEMQMILGLLSIKIVSTNKTWLLQVPPFRVDVQR
                     PADIAEEILRIYGYNRVPLPARMSSSLNPAPKPDRDAVRHTVSDLLSSNGFFEILSNS
                     LTRSDYASLAGSEQMKEEWHVRLLNPLSSELDVMRQTLLFSGLEAISHNLNRQQSDLR
                     FYEFGKIYFHFGNGKYEEQQRLSLFLTGRRETESWNASSNDVSFFTMKSAVMNVFARL
                     GLNAALRTVALDNTWFEDGQALEIAKKKVAEFGWIRGAITKKMDIRQTVFYADIHWDV
                     VMGLLHMNKVKYKEISKFPEVRRDLSLLLDEKITFSEIENIARQSEKKLLREVGLFDV
                     YQGKNLESGKKSYALRFVLQDPEQTLTDQVIDKTMERIQQSLEQQLGAQLRS"
     gene            1885..3180
                     /locus_tag="PK610_08115"
     CDS             1885..3180
                     /locus_tag="PK610_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRJ35939.1"
                     /translation="MYNLLYPHMNFFVKRFFRQFRYYGFEKIPKNEAVILAPNHQNAF
                     LDAIVMTYGLGWKNQLSFLVRASIFKKPLAAKFLYSLNMLPVYRKDHDGMENMDKNDE
                     VFDNCIYLLKNKRPLMLFPEATHTLRRRLLPLKKGVTRIAFSAEEQNDFALGVKIYPI
                     GINYSTPRDFYGDLLMKVGEPIHVSEYLELYKESPAKAHARIRTELEARMRELMIDIR
                     TDEHYELVEGWRQMDANNKGIDDVEKEFDNAKQLIARAEKFIGEHPEEVGEWHQKMAE
                     YRHLLNRNKLSDKHFSPSAPRFSPVLSGLLTVLLSPLIVFGGIHNFFPFVAARYIVKK
                     YIKDPGFQSSIKVAVAAFMFPVLHILCCLIYWMLSGSFGGALLYWFVILFTGEVALWY
                     YLKWEQFNLSRRTAAFIQTPDGKEAVSLRRDLSLVLDRF"
     gene            3211..4311
                     /locus_tag="PK610_08120"
     CDS             3211..4311
                     /locus_tag="PK610_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRJ35940.1"
                     /translation="MRTFFAFLLVALVGQAHSSGDTLRSYRVNDFFRAIPELDQRVEE
                     VFLRLSDEQRVAQMIMPAAGKLGKSTAHVQRLIREGKIGGAILLNGTKTEFTNFSRQF
                     DSISVANGHPRLLYSADAELSLINMKIKETQKVKYANKIRSFDVLRKETEKICAELNA
                     MGVQWNFAPVVDLSPNSTVSFRSFGLNKDTIIQFSNEFIRISQARNIIATAKHFPGHG
                     YVVGDTHKQLVFIDGEMKEVDVYKPLIRDSVLSIMIGHIAVKNNKKYDTEGLPSTVSR
                     KIVTGLLREEMGFRGLIVTDAMGMGGVVNVPQCGLKAAKAGCDVILMPRDEDAVHADI
                     LAEIKKDEVFRDQVYESVKRIIRIKIILGILS"
     gene            4344..5207
                     /locus_tag="PK610_08125"
     CDS             4344..5207
                     /locus_tag="PK610_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRJ35941.1"
                     /translation="MMKSSDGAQISAAQSAIDVKTVIEQLFTGMRTGDSSLVRQVFHV
                     SARLQSVVEQKGIPQLATESVDEFVKAVGTPHPEIWDEQIRSWKIDIDGDLASVWTDY
                     EFYLGKKYLHCGVNAFHLFKTAMGWKITQITDTRKKECQQSEKGISITEKVKPFELEI
                     NKVMDEWHMAAGKGDEDVFFGTMAEDFIYLGTDKTERWTKTKFWDFAKKYFERKEGAW
                     KFKPLSRKIYFTDDLSYAWFEEHLDTWMGVCRGSGVLRFSEGKWMLKHYNLSVTINND
                     KVQEFIKLKPN"
BASE COUNT         1467 a         1008 c         1280 g         1453 t
ORIGIN      
        1 gcggtgataa ggcggaattg gaatttcctc atcagaaacc acttgattta aaaaaaggaa
       61 ttttccctgt aaaagtgcgt gtagaagatc aggaatcctg taaacgatat gcaggcatta
      121 ccataggaaa tattaaagtt gctccttcac ctcagtggtt gcaaaatcgt ttaaaagcga
      181 tcggtgcgcg tcccatcaac aatgtggtgg atattaccaa ttttgtaatg catgaacttg
      241 gtcagccact gcatgctttt gatgctgctg ccattggagg aaataccatt gttgtacgca
      301 aagcaaaaag cggaaaacgt ttcattactc ttgatgatgt ggaacgcgaa ttgcgcgatg
      361 tggataccat gatttgcgat gaggagaaag aactttgcat tgcaggtgtg tttggcggat
      421 cgaagtcggg tgtaaatgat tccacgcaac gtatctttct cgagagcgcc tggttcgatc
      481 ctgcgcatgt gcgcaaaact gcacgtggtc atggtttgaa taccgattct tccttccgct
      541 ttgaacgagg tgccgatccg gatatggttg tacctgcctt gcatcgcgct gctgaattga
      601 tcgttgaact atgtggtgga gttgtggaat cagatatcag cgatttttat cctgtgcctt
      661 tttccccatt cgaggtggat atcgatccgg aacaaatcaa taaactcatc ggaaccacaa
      721 tttctccgga tgagatgcaa atgattctcg gattgttatc cataaaaatt gtttcaacca
      781 acaaaacctg gttactgcag gttccgccat tccgagtgga tgttcaacgt cctgccgaca
      841 ttgcggagga aattttgcgt atttacggat acaacagggt tcctcttcct gcgcgaatga
      901 gttcttcttt aaatcctgct ccaaaacccg atagggatgc ggttcgccat actgtttcgg
      961 atctgttatc ttccaacgga tttttcgaga tcctctccaa ttcacttacg cgtagcgatt
     1021 atgcttctct tgcgggaagt gaacagatga aagaggaatg gcatgtgcgt ctgctcaatc
     1081 cactgagctc tgaactggat gtgatgcgtc agactttgct tttttcggga ttggaggcga
     1141 tttcgcataa cctgaatcgt caacagtcgg atcttcgttt ttacgaattc ggaaaaatat
     1201 attttcattt tggcaacgga aaatacgagg agcaacaacg cttatcgctt ttcctaaccg
     1261 gcagaagaga aactgaaagc tggaacgcgt cttccaatga tgtttccttt tttacaatga
     1321 aatcggcagt gatgaatgtc tttgcccgac taggattgaa tgctgcttta cgcacagttg
     1381 cattggataa cacttggttc gaagatggtc aggcgctgga gattgcgaag aaaaaagtag
     1441 cagaattcgg atggatccgt ggtgcgatca ccaaaaagat ggatatccgt cagactgtat
     1501 tttatgccga tattcattgg gatgtggtga tgggattgtt gcacatgaac aaagtgaagt
     1561 acaaggaaat ttctaagttc cctgaagtcc gtcgcgatct ttcactcttg ctcgatgaaa
     1621 aaatcacttt ctccgaaatc gaaaacattg ctcgtcaatc ggagaaaaaa cttttacggg
     1681 aagttggatt gttcgatgtt tatcagggaa aaaatctgga aagcggaaag aaatcctatg
     1741 ccttgcgttt tgttttgcag gaccctgaac agacacttac cgatcaggtg atcgataaga
     1801 caatggaaag gattcaacaa tcactggagc aacaattggg agctcaactt cgatcgtaat
     1861 atgcgcgtaa ccggaaacag atggatgtat aacctgctgt acccgcacat gaattttttc
     1921 gtgaagcggt tcttcaggca attccgctat tacggattcg aaaaaattcc caaaaacgaa
     1981 gcggtcatcc tcgcccccaa tcaccagaac gcgtttttag atgctattgt gatgacttat
     2041 ggtcttggct ggaaaaatca gttaagcttt ctggtgcgtg cttccatttt taaaaaacca
     2101 ttggcagcaa aatttcttta ttcactcaat atgcttcctg tgtaccgaaa ggatcatgac
     2161 ggcatggaga atatggataa gaacgatgag gtatttgata attgtattta tctgcttaag
     2221 aataaacgtc cgctgatgct tttcccggaa gcaacgcata cgttacgccg acgtttactt
     2281 ccgctgaaaa aaggggttac acgaattgca ttcagtgccg aagaacaaaa tgattttgct
     2341 ttgggggtga aaatttatcc cattggaatt aattattcta ctccccgcga tttctacggc
     2401 gatctgctga tgaaagtggg tgagccgatt cacgtcagcg agtacctcga attgtacaag
     2461 gaaagtcctg ctaaggcgca cgcacgtatc cgtacagaac tggaagcccg tatgcgtgaa
     2521 ctcatgattg atattcgcac ggatgaacat tacgagctgg ttgaaggatg gcgtcagatg
     2581 gatgccaata acaaggggat tgatgatgtg gagaaagaat ttgacaatgc aaaacagttg
     2641 atcgcacgcg ctgaaaaatt tattggggaa catccggagg aagtcgggga atggcatcag
     2701 aaaatggccg aataccgtca tctgctgaat agaaataaac tgagcgataa acatttttct
     2761 ccttctgcac cgcgtttcag tccggtttta tcgggattgc taactgtgct gttgtcgcca
     2821 ttaatcgttt ttggaggaat tcataatttc tttccttttg tagctgcgcg ttatatcgta
     2881 aaaaaataca tcaaggatcc cggttttcag agctccatta aggttgccgt ggcggcattt
     2941 atgttcccgg tattgcatat tttgtgttgc ctgatttact ggatgcttag cggttctttt
     3001 ggtggagcac tcctgtattg gtttgttatt ctgtttaccg gtgaggtagc actatggtat
     3061 tacctgaaat gggaacaatt caatctttcc cgaagaacag cagcatttat tcaaacaccc
     3121 gatggcaagg aagcggtttc cctgcgtcgt gatttatccc ttgttcttga tcgcttttga
     3181 tttgggcaag gtatttgcag ttttacatgt atgagaacat tttttgcctt tttgcttgtt
     3241 gcgttagtcg gacaagccca cagttccggt gacaccttgc gtagttatcg tgtaaatgat
     3301 tttttccgag caataccgga gttggatcag cgtgtggaag aagtttttct gcgtctgagc
     3361 gatgaacaac gcgttgcgca aatgatcatg cctgctgcag gtaaactcgg taaatcgact
     3421 gcacatgtgc aacgactcat ccgcgaggga aaaatcggag gtgctatttt gctgaatggt
     3481 accaaaacgg aattcactaa tttctcccgc cagtttgata gtatttccgt cgcgaacgga
     3541 cacccgcgtt tactgtattc ggccgatgca gaactgagtc tgatcaatat gaagatcaag
     3601 gaaacacaaa aagtaaaata cgcgaacaag atccgctcat tcgatgtgct gcgaaaggaa
     3661 acagaaaaga tttgtgccga gctgaatgca atgggcgtgc agtggaattt tgctccggtg
     3721 gttgatcttt cacccaattc tacggtaagc ttccggagtt tcggattgaa taaggatacc
     3781 atcattcagt tttcgaatga attcatccgt atctcccagg caagaaatat tatcgctact
     3841 gcaaaacatt ttcccggaca tggatacgtg gtaggtgata cgcacaaaca actggtgttt
     3901 atcgatggag aaatgaaaga agtggatgta tacaagcctc ttatcagaga cagtgtattg
     3961 tcgatcatga ttggacatat cgcggttaaa aacaataaaa agtacgacac cgaaggattg
     4021 ccatcaaccg taagccggaa aattgtaacc ggattattgc gtgaggaaat gggttttcgc
     4081 ggattgatcg ttacggatgc gatgggaatg ggaggagtgg tgaatgtacc gcaatgtgga
     4141 ttgaaagcag ccaaagcagg ttgtgatgta attctaatgc cgcgtgatga agatgctgtg
     4201 catgccgata ttttggctga gataaaaaag gatgaagtct tccgcgatca ggtgtatgaa
     4261 agcgtgaagc ggatcatccg aatcaaaata attttaggca tcctctcatg aggcgctttt
     4321 tccttttagg atttgctttc ctcatgatga aatcatcaga cggagcacag atctctgctg
     4381 cacaatctgc cattgatgtg aagacggtca ttgagcaatt atttaccgga atgcgaacag
     4441 gtgatagcag cttggtccga caagtctttc atgtatccgc gcgcttgcag agtgtggtgg
     4501 aacaaaaagg aattccccaa ctggcaacgg agtcggtcga tgaatttgta aaggcagtag
     4561 gaacaccaca tcccgagatc tgggatgagc agatccgcag ctggaaaatt gatatcgacg
     4621 gcgatctggc atctgtatgg accgattatg aattttatct gggtaaaaaa tatttgcatt
     4681 gcggtgtaaa tgcatttcat ctgtttaaaa cagcaatggg atggaagatc acacagataa
     4741 ccgacacaag aaaaaaggaa tgtcagcaat cggaaaaagg gatttccatt actgaaaaag
     4801 taaagccatt cgaattggag atcaataagg tgatggatga atggcacatg gctgcaggaa
     4861 aaggcgatga agatgttttt ttcgggacaa tggcggaaga ttttatttac ctcggtacgg
     4921 ataaaaccga acgctggaca aaaacaaaat tttgggattt tgcaaagaag tattttgagc
     4981 gtaaggaagg agcatggaag tttaaaccgt tgtcgcgcaa gatctatttc accgatgatt
     5041 tgtcgtacgc ctggttcgaa gaacatctcg atacctggat gggtgtgtgc aggggaagcg
     5101 gtgtattgcg tttctcagaa gggaaatgga tgctgaagca ttataatttg tcggtgacga
     5161 tcaataacga caaggtgcag gagtttatta agctgaagcc gaattagg
//
LOCUS       DAOMYG010000222         5193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5513_length_5193_cov_12.992215, whole genome shotgun sequence.
ACCESSION   DAOMYG010000222 DAOMYG010000000
VERSION     DAOMYG010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5513_length_5193_cov_12.992215"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..4274
                     /locus_tag="PK610_08130"
     CDS             63..4274
                     /locus_tag="PK610_08130"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ35942.1"
                     /translation="MKKENLIFNRVNFSAVFTYSKATLLTLLLFIVFSNSNAQTFTYN
                     AWVAGSTSYSTTAGGNTMSVAYTESANNRTNSLASTTCFSSPTTLPNIPFYYNVATMN
                     IADYSSSTCYCSASNAQSGLVLAANWANNNATNFEQITITFTNPVCGATFNIWDINQN
                     FWSGDGSTYFTDKIDISGTDASSNPITAANIGISNCGSNTVTTSGNTKTITGVQNGCN
                     CGSHSVTLSGTVKTITIKYYPGGSTYNANPWSQYVILTSVVATAAPTAGITPGALACG
                     SSSTTLTATTNASSPTYAWTGPGGSTITNPNAASTSVTGAGTYTVTITSTAGCTNTAT
                     YTLTPSGSAPNLTAANDQTVTCSNPTISASSTDPVTYSWSGPGIVSGGATSSPTVNQS
                     GSYTVIVTNTLNGCTNTATINVTANTSPPGVTPGSPLALNCSTSSGTITATPGIPNVN
                     YLWSGPGIISGGTSQTPTVNAAGTYTVTVTSTTNGCTSTATVAVTSNTTPPTAIMGAP
                     FSLTCTTLSGNVSVTTNAGSPSYLWAGPGIVSGGTTSSPVVNASGTYSVTVTNLANGC
                     TNTGSVSISSNTAQPNVTAGSPLTLNCIILSGQISASTGIANPSYSWSGPGIVSGGTT
                     SSPTVNSAGSYVVTVTNPANGCTGTTTVAVTTNTNSPAVTASSSLVLNCSTTSGTVNA
                     STPVNNPSYLWSGPGIVSGGNTSSPTVNATGSYTVTITDPANGCTGTSSVSVSGNFTT
                     PNVAPGSAQALSCVSPTVSISASSSTGGAQFTWTDGSGNLAGSTPTSTSTTVGVAGTY
                     TVTVTNPINGCTSTATITVSPNAGQPQITIDPAPSITCVSSSATITGSSSTTNVSYAW
                     TDASGNPAGSNPTQTSTTVSTPGTYTLTITDNGTGCSSIGNITVVLNNQPPTVTIANP
                     SLMSCDFTPVSLSGITNAGNASFAWTNNSGNPAGTTPSAISTNVNAPGEYTITVLNLD
                     NGCSTSAMVTVELENAPIAFVASASDLTCSLVSISLDGTGSATGTDIEYSWSGPGIIT
                     GNSTMNPLVNLPGIYTITVTNATTGCFSTASVTVGQNILLPIADAGSSGSINCIASIV
                     DLDASLSTGNNISYFWSGPGVVTGNNLAQATANLAGWYFILVTSGDNGCTALDSVEIM
                     IDTIVPQLTTGGDSQIGCVSSTTQLSASGNGTTYVWSGPGILSGGNTATPIINQAGEY
                     IVVSTGSNGCTSSDTLNVSQVIGPIADFSVTPNQGPSPLLVTTTNNSSGNGISYLWNL
                     GNGTSSSALNPTTTYNVPGTYSIVLLVTDAAGCTDTTIRTIIVDGLPIDIPNGFTPNG
                     DGFNDYFVIVGLHQYPENKITIFNRWGDPVFSASPYLNNWDGSTSNEKLRISGDKVVD
                     GTYFFILELGAGLEPISGFVELKTR"
     gene            4279..>5193
                     /locus_tag="PK610_08135"
     CDS             4279..>5193
                     /locus_tag="PK610_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRJ35943.1"
                     /translation="MKRTLLYLLVFISVQAFGQNQINMGQYMVHQPFMNPASISSYSK
                     VTFAGFYRSQWTQFDGAPVTQGFNAIVPLKRNKHTVSFTAFHDAIGINNNSEVSASYA
                     YSMKTGLNAYLSFGISASLDLLQADYAKLHTIDPNDPLFQSASPLVPLPDFKFGMYFH
                     KNRFYAGLALPNFMNNKIEFTSSGASKGTSGFDFDNLHFYLHTGYAFRLNDKWDLNTS
                     TLIKEVSGAPVQFDLNAQLMFKRIIGFGTSYRSSREILGMITAQIIPTLKMSYGYEYN
                     IGQIGKFSNGSHEILLIYQLNPPKEPVVS"
BASE COUNT         1543 a         1113 c          991 g         1546 t
ORIGIN      
        1 gggtggcact tctttttaaa gccggttaag aattgaatat tgtatgtaga aaattctaaa
       61 ctatgaaaaa ggagaatttg atttttaacc gcgttaattt ttccgctgtt tttacttatt
      121 caaaagcaac gctactaaca ttattgcttt ttattgtctt ttcaaactct aatgcccaga
      181 cttttacata caatgcatgg gtagctggaa gcacttccta ttccacaacc gcaggaggta
      241 atacaatgtc cgttgcatat acagaatctg ctaataacag aaccaattct ttggccagta
      301 caacctgctt tagtagccca acaacgcttc ctaatattcc gttttattac aatgtggcaa
      361 ccatgaatat tgccgattac tcatcgtcca catgctattg ttctgcatcc aatgcacaaa
      421 gtggtctcgt gcttgctgca aattgggcga ataacaacgc aacaaatttt gaacaaatca
      481 ctatcacgtt tactaatccg gtttgtgggg cgacatttaa tatttgggat attaatcaaa
      541 atttctggag cggtgatgga tcgacttatt ttacagataa aatagatata tcgggaacag
      601 atgcttcatc aaatccaatt acagcagcca atattggtat tagtaattgc ggttcaaata
      661 cggtaacaac tagtggaaat accaaaacaa ttaccggggt tcagaatggc tgtaattgtg
      721 gttctcattc tgttactctt tcaggtacag taaaaacaat tacaatcaaa tattatcccg
      781 gtggatctac ctacaatgct aatccatggt ctcaatatgt tattttaact tcggtggttg
      841 caacagcggc accaacagca ggcattactc ctggagcttt ggcgtgtggt tcttcaagta
      901 ccacattaac agcaacaact aatgcctctt cacctactta tgcgtggaca gggccaggtg
      961 gctctaccat tacaaatcct aatgcagcga gtacctcagt aacaggtgca ggtacttata
     1021 cggttacaat aacttcaact gcaggttgta caaatactgc tacatatact ttaacgcctt
     1081 ccggatcagc accgaattta acagcagcaa atgatcagac tgttacttgt tccaatccaa
     1141 caatttctgc aagctcaaca gatcctgtaa cctattcttg gtcgggtccg ggaattgtta
     1201 gcggtggggc tacatctagt ccaacagtaa atcaaagtgg ttcctatacc gtaattgtaa
     1261 cgaatacatt aaacggttgt acaaacacag ctacaattaa tgttactgca aatacgagtc
     1321 caccgggagt tacgccagga tctcctttag cactcaattg ttcaacatct agcggtacaa
     1381 ttactgcaac accgggaatt cccaatgtca attatttatg gtccggaccc ggaattataa
     1441 gcggaggaac aagtcaaaca ccaactgtaa atgctgccgg aacttatact gttactgtta
     1501 ccagcacaac caatggatgt acatcaacag caacagttgc tgttacttcc aatacaacac
     1561 ctcccactgc aataatgggt gctccttttt cgctaacctg tacaacatta agtggcaatg
     1621 tttcggtaac tacaaatgca ggttcaccat cttatttgtg ggctggtccc ggaatagtaa
     1681 gcggggggac aacttcttct ccggtagtaa atgcatcggg tacatattcg gttactgtca
     1741 caaaccttgc taatggttgc acgaatacgg gatccgtttc catttcgtcc aacactgctc
     1801 agcccaacgt aacagccgga tcaccattaa cgcttaattg tataatatta tccggacaaa
     1861 tttctgcttc aacaggaatt gcaaatccat cctattcctg gagtggacct ggaattgtta
     1921 gtggaggtac aacttcaagc ccaactgtta attctgcagg aagctacgta gttactgtga
     1981 ctaatccagc aaatggatgt acgggaacaa caactgtcgc agtaactacc aatacaaatt
     2041 cgcctgcagt aacagcatca tcttcattgg tgctcaattg tagtacaacc agcggaacag
     2101 tgaacgcatc aacaccagta aataatcctt cttatttgtg gagcggaccg ggaatagtta
     2161 gtggaggaaa tacttcttct ccaacagtaa atgctacagg ctcatatacg gttacaataa
     2221 ctgatcctgc caacggttgc acgggaacct ccagtgtttc tgtttcaggc aactttacta
     2281 ccccgaatgt agctccaggg tctgcacaag ctctaagttg tgtttcaccc actgtatcaa
     2341 tttcagcgtc ctcatcaact ggaggtgcgc aatttacatg gactgacggc tccggaaatc
     2401 ttgcaggatc aacacctaca agtacttcaa caactgtagg tgtagcaggc acatatacag
     2461 ttacagttac caatccaatt aatggatgta catcaacagc aacaattaca gtttctccaa
     2521 atgcaggtca gccacagata acaatagatc ctgcgccatc tattacttgc gtctcttctt
     2581 ctgcaacaat aacaggaagt tcaagtacta caaacgtaag ttatgcatgg acagatgcat
     2641 ctggaaatcc tgcaggatca aatcccactc aaacttcaac tacagtatcc actcccggaa
     2701 catatacgct cactattacg gataatggta caggatgctc tagtattgga aacattacag
     2761 ttgttttaaa taatcagcca cctacagtta caatcgcaaa tccttcattg atgagttgtg
     2821 actttacacc tgtttcgctt tcaggaatta caaatgcagg caatgcttca tttgcgtgga
     2881 caaacaattc cggtaatcct gctggtacaa ctccatcagc aataagtacc aacgtaaatg
     2941 ctcctgggga atatacaatt acagtattaa atcttgataa tggctgttca acttcagcaa
     3001 tggttactgt agagttggaa aacgcaccaa ttgcttttgt tgcaagtgca tcagatctca
     3061 catgttcttt agtcagcatt tctcttgatg gaacaggttc agcaaccgga acggatattg
     3121 agtattcatg gagcggaccg ggaataatta ccggaaattc aactatgaat ccccttgtga
     3181 atttaccggg gatttataca ataacagtta caaacgcaac tacaggttgt ttttctacag
     3241 caagtgttac cgttggacaa aacatccttc tgccaatagc cgacgcagga tcatctggaa
     3301 gtattaattg catagcatct attgttgatc tggatgcatc attaagcact ggaaataata
     3361 tatcctattt ctggtcaggc ccaggtgtag ttaccggaaa caatcttgcc caagcaacag
     3421 ccaatctcgc tggatggtat tttatactag ttacaagtgg cgataatggt tgcacagctt
     3481 tggattctgt tgagattatg atagatacaa ttgtacctca attaacaacc ggaggtgatt
     3541 cacaaatagg ttgtgtatcc tctacaactc aacttagtgc atcaggaaat ggaacaacct
     3601 atgtctggtc aggacctgga attcttagtg gtggcaatac cgcaacacct ataattaatc
     3661 aggcaggtga atatattgtt gtttcaaccg gatcgaatgg ctgtacttca tcagatactt
     3721 taaacgtctc acaggtaatc ggtcctattg ctgatttctc ggttactcca aatcagggac
     3781 cgtctccatt gctagttaca acaactaaca attcatctgg aaatggaata tcctatttat
     3841 ggaacttggg taatggcaca tcttcaagcg cgctaaatcc tacaacaacc tataatgttc
     3901 cgggaaccta ttccatagta ttgcttgtta ctgatgctgc tggttgtaca gacacaacaa
     3961 tacgcactat tattgtggat ggacttccta tcgatattcc aaatggtttt acaccaaatg
     4021 gcgatgggtt taacgactat tttgtaattg tgggattgca tcaataccct gaaaacaaaa
     4081 taacaatatt caaccggtgg ggagatcctg ttttttcagc gagtccttac ctgaacaatt
     4141 gggatggatc tacctctaac gagaagttgc gtatttcagg tgataaagta gttgatggaa
     4201 cttacttttt tatactggaa ttaggggctg gtttagaacc tatatctggg ttcgttgaat
     4261 taaaaacacg ttgatactat gaaaaggact ttactgtatt tattggtgtt tatttctgtt
     4321 caggcttttg gtcagaacca aattaacatg ggtcaatata tggtgcacca acccttcatg
     4381 aatccggcct cgatatccag ttattctaaa gtaacgtttg ctgggtttta tcgttcacaa
     4441 tggactcaat tcgatggggc tcctgtaacc cagggattca atgcaattgt tccgcttaag
     4501 cgcaataagc acacagtttc gtttactgcc tttcatgatg caattggaat taacaataat
     4561 tcggaagttt ccgcatctta tgcttattcg atgaaaactg gtttaaatgc atatttgtcg
     4621 tttggcattt ctgcaagcct tgatttgttg caggccgatt atgctaagct gcataccatt
     4681 gaccccaatg accctctatt tcaatcagca tctccgctcg ttcctctacc tgattttaag
     4741 tttggaatgt atttccataa aaatcgtttt tatgcaggtc ttgcactacc taatttcatg
     4801 aataataaaa ttgaattcac atcttcagga gcatccaaag gaacttcagg ttttgatttt
     4861 gataatcttc atttctattt gcatactgga tatgcattca ggttaaatga taaatgggat
     4921 ttgaatactt ctacgttaat aaaagaagtt tccggtgcac cggttcaatt tgatcttaat
     4981 gcccagctta tgtttaagcg cataattggt tttggaactt cctatcgcag tagtcgggaa
     5041 atcttaggta tgataacagc gcaaatcatt ccgactctta aaatgtctta tggatacgaa
     5101 tataacatcg gacaaattgg aaaattctca aatggatccc acgaaatctt gttgatttac
     5161 caattgaatc ccccaaagga acctgttgtt tct
//
LOCUS       DAOMYG010000223         5182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5528_length_5182_cov_8.968012, whole genome shotgun sequence.
ACCESSION   DAOMYG010000223 DAOMYG010000000
VERSION     DAOMYG010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5528_length_5182_cov_8.968012"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..473)
                     /locus_tag="PK610_08140"
     CDS             complement(12..473)
                     /locus_tag="PK610_08140"
                     /inference="COORDINATES: protein motif:HMM:NF025454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HRJ35944.1"
                     /translation="MYPKGYASQYRQEFFEFLKLKMSRRLSSEKVRINWMKYKTGVDD
                     LYFRCEIDRSSARFCIDIQHRDPGVRELVYNQFLEMKTVLHQHVGEMYWLKDHHTEDG
                     LEISRIYAELPEANLFEKESWPEAFEFFEKKLRKMDKTWSMAKFTFFQLLK"
     gene            complement(477..821)
                     /locus_tag="PK610_08145"
     CDS             complement(477..821)
                     /locus_tag="PK610_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRJ35945.1"
                     /translation="MKYRILLKAAQVANAQKPLSLQKKKFMATMNDFCQSCSMPMAKD
                     LQRGGTNADGSKSKMNCSYCFQNGKFIDNFTTAKEMQQFLRKKFKEQGFGPLRRWFYT
                     SYIPRLERWKKK"
     gene            862..4053
                     /locus_tag="PK610_08150"
     CDS             862..4053
                     /locus_tag="PK610_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRJ35946.1"
                     /translation="MIRSLILLFLLLVSQTRALSELHAQTYLVIRPQGHKVQTREMCI
                     SKDGKTIFTGDFDKTIIAWDAANGMPIREYLGQIGPGNEGMVYALALSPDNLVLAAGG
                     WFGKDDESENLGDIRIYDVASGKIKKVIHGHANVINDLAFTADSKNVIAVDADGRIVQ
                     WNCSTGALVREYNYAKEVDVKDVFVNGSNFVTGDDKGNLVLWSTENSKPIKKDAFYKG
                     FQIRDVVISPDGKWIACSGERFISVYNEKLKRIADIDNEVVASFLRFSPDSKKILAGC
                     STTGKEHHCNVFALENNAWVEYGSYDGHTSSVICGGFINDQTMVTSGGESNEIVVWTI
                     KAKSEKAKVMRTMRGVGQSFFAAALADKQIAYTTTWTENFGLSHYTRQFDLVGRKDAK
                     IDSKTSWNRPLTQLEKYSLVRARGGVEMEQLNSKLLIQRDRKIVDSIVREYWNGSRHN
                     VFGFLPENWIVSGGSYGILSAYDLTGNEVSRFVGHEGDVWSLSVSADGKRMITASSDQ
                     TIRIWPLHEVGKPTTTSLPLSPMEYSNELEVGPTFKKVFQQLGVMREADGKSIADWRK
                     VIQVLNQNDFSCQFLENKLSESTRNFIYPVYSLFISTEGEWVVWNQEGYFTSSRRGSK
                     YVGYHINQGKEKEARFYPFEQFDLKYNRPDILLKDIGGVAPEIIEGYQLAYKKRLRKM
                     GFKEEQLGVQIHIPEIEIVQHSVNSGKAKIAFRASDSEFQLERLAISINDVPLYGSKG
                     KKIEGAKLESELEIELMPGRNKVQLSVINSRGAESMRETVVLHSGQEQLPELYLLSIG
                     VSEYKDKKFNLRYAAKDANDISELASRSTAFSKVNQLVLVNEKVDLLALEKAKTEFLS
                     RAKRNDVVMVFVAGHGVLDKDLNYFFASHTIDFNQPSKNGIPYEKLEELLDGLTAIRK
                     ILLMDTCHSGELDKEENEAGTQSAEVQSDISFRNAGTIGVVSKGGFGIKQSSQLATEL
                     FTDLRIGTGALVISSSGGSEYAMESAQWKNGLFTYSLLNGIASGKADVNHDQQITIGE
                     LQVYVAEQVQSLSGGAQQPAFRRENLEFDVRVW"
     gene            4148..4879
                     /gene="pgeF"
                     /locus_tag="PK610_08155"
     CDS             4148..4879
                     /gene="pgeF"
                     /locus_tag="PK610_08155"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HRJ35947.1"
                     /translation="MNLVQHPSGIQWYNAGQIAVPHAFFLRHGGVSPTPCDALNFSGS
                     LDEEENVKENRRRAFDALGWDIEKAAYLKQIHSTIICDAESGQQEGDALVTNKKGIPI
                     AVSGADCFPVLFHDPVISVIAAAHCGWKGTINRLAGKVILRMQELNCKPEHIHVAIGP
                     GISRMAYEVSPELIDQFREAGFSDHSFSGRHLDLAQCIRQNLMESGISEAHIQTLARC
                     TTDADFFSHRRDNGRTGRLWSVICL"
BASE COUNT         1488 a         1031 c         1223 g         1440 t
ORIGIN      
        1 gaaaatgatg attatttgag caattgaaaa aaggtgaact tggccatcga ccaggtctta
       61 tccattttgc gcaacttttt ttcgaagaat tcaaaagcct ccggccagct ttccttttca
      121 aataaattgg cttcaggaag ctctgcgtaa atgcgcgaaa tttccagtcc gtcctcggtg
      181 tgatggtctt tcaaccaata catctcacca acatgctggt gcaggacagt cttcatttcc
      241 agaaattgat tgtaaactaa ttcccgtact ccgggatcgc ggtgctgaat gtcgatgcag
      301 aaacgcgcac tgctgcggtc gatctcgcaa cggaaataca aatcatcaac accggttttg
      361 tatttcatcc aattgatacg caccttctcc gaagaaagtc gtcgtgacat tttcagtttc
      421 agaaattcaa agaattcctg acggtattgg gaggcgtaac cttttggata catgatttat
      481 ttctttttcc aacgctccaa acgcggaata taggaggtat aaaaccaacg acgcaaggga
      541 ccaaatccct gttccttgaa tttttttctc aaaaattgct gcatctcttt tgcagtagtg
      601 aaattatcga tgaatttacc gttctgaaaa caataactgc aattcatctt gcttttactg
      661 ccatcagcat tggttcctcc gcgctgaaga tcttttgcca ttggcatgct gcagctttga
      721 caaaagtcat tcattgtagc cataaacttc tttttttgta aagataaggg tttctgagca
      781 ttcgctacct gagcggcttt cagtaaaata cggtatttca cggaggacag tttccggaat
      841 tttgacatac tttcatttcc tatgatccga tcacttatcc tgctatttct gctccttgtt
      901 tcgcaaacaa gagcactaag cgaactgcat gcgcaaacct atcttgtaat tcgtccgcag
      961 ggacataagg ttcagacaag ggagatgtgt atcagcaagg atggtaagac catttttacc
     1021 ggcgattttg ataaaaccat cattgcatgg gatgctgcaa acggaatgcc gatacgtgaa
     1081 tacctgggac agattggtcc cggtaacgag gggatggttt atgcactggc tttgtcgccg
     1141 gataatttag tgttggccgc aggaggatgg ttcggcaaag acgatgaatc ggagaacctg
     1201 ggcgacattc gaatctatga tgtagcaagc ggaaaaatta aaaaggtaat tcatggacat
     1261 gccaatgtga tcaatgatct tgcatttact gccgacagta aaaatgtaat tgcagtagat
     1321 gccgacggac gaatagttca atggaattgt tcaacgggcg ctttggtgcg cgaatacaac
     1381 tatgcgaaag aagtggatgt gaaagatgtg ttcgtgaatg gatcgaattt tgtaaccggc
     1441 gatgataagg gcaatctggt attgtggtcg accgaaaaca gtaaacccat taagaaagat
     1501 gcattttaca aaggatttca gatccgcgat gtggtaattt ctcccgatgg aaagtggatc
     1561 gcctgttcgg gagaacgatt tatttcggtg tacaatgaaa aactgaaacg cattgctgat
     1621 atcgacaatg aagtggtcgc atcttttctg cgtttttcac ccgactccaa aaaaattctt
     1681 gcaggatgtt ctactaccgg aaaagaacac cattgcaatg tgtttgcact cgaaaacaat
     1741 gcctgggttg aatatggaag ttatgatggt cataccagtt ctgtgatatg cggtggattc
     1801 attaacgatc aaacgatggt cacttcggga ggagaaagca atgagattgt ggtttggacc
     1861 atcaaagcga aatcggaaaa ggcaaaagta atgcgaacaa tgcgtggagt agggcagtcc
     1921 tttttcgctg cggcacttgc ggataaacaa attgcttata ccaccacatg gacagaaaat
     1981 tttggtctct cgcattatac ccggcaattt gatttagtgg gaagaaaaga cgcaaaaatt
     2041 gattcaaaaa caagttggaa cagaccgctt acacaactgg aaaagtattc cctggtacgc
     2101 gcacggggtg gagtagagat ggagcagttg aattccaaat tactgattca gcgcgatcgt
     2161 aaaattgtcg attccattgt gcgtgaatac tggaatggtt ctcggcataa cgtattcgga
     2221 tttctccccg aaaattggat cgtttccgga ggttcctacg gaattttgag tgcctacgat
     2281 ctaacgggga atgaagtctc gcgttttgtc ggacatgaag gagatgtgtg gagtttgtcg
     2341 gtttctgccg atggtaaacg catgatcact gcatcttctg atcaaaccat tcgcatctgg
     2401 ccactgcatg aagttggaaa accaactact acttctttgc cgctttcacc aatggaatat
     2461 tccaatgagc ttgaagtagg acccaccttt aaaaaagtat ttcagcaatt gggagtaatg
     2521 cgtgaagctg atggtaaaag cattgctgat tggagaaaag tgattcaggt tttgaatcaa
     2581 aacgattttt catgtcagtt tctcgagaat aaattatcgg aatcaacacg gaattttatt
     2641 tatccggttt actccctttt catttccacc gaaggagaat gggtcgtatg gaatcaggaa
     2701 ggttatttta cctcctcccg ccgcggatcg aaatatgttg gttaccacat caatcaggga
     2761 aaagagaaag aggcgcgttt ttatcctttc gaacaatttg atctcaaata caatcgtccg
     2821 gatatccttc tcaaagatat tggcggggta gctcctgaaa tcattgaagg ctatcagttg
     2881 gcttataaaa aacgattgcg caaaatggga ttcaaagagg aacaattggg tgtgcaaatt
     2941 catattccgg agattgagat cgtgcagcat tcagtaaatt ccggaaaagc gaaaattgca
     3001 tttcgagcaa gtgattcgga gtttcagctc gaacgtttag cgattagcat taatgatgtt
     3061 cccctatacg gatcgaaagg aaaaaaaatt gagggcgcaa aactggaatc ggaattggaa
     3121 atagaactga tgcccggaag aaacaaagtg cagctaagcg tgatcaatag caggggagca
     3181 gaatcaatgc gcgaaaccgt tgtgctgcat tcaggtcagg agcaattgcc tgaattgtat
     3241 ttgctttcta tcggtgtaag cgagtacaaa gacaaaaaat tcaatctgcg ttatgctgct
     3301 aaagatgcaa atgatatttc cgaattggca tcacgaagca cggcattttc taaagtgaat
     3361 cagcttgtgc tcgtcaatga aaaagtagat ctgcttgcat tggaaaaagc aaagactgag
     3421 tttttatcca gggcaaaacg caatgatgtg gtgatggtgt ttgtggcggg acacggagtt
     3481 ttggataagg acctgaatta ttttttcgct tcacacacca tcgatttcaa ccagccgtcg
     3541 aaaaatggaa ttccctatga gaaactggaa gaattactgg atggtttaac tgcgatccgc
     3601 aaaatattac tgatggatac ctgtcattcc ggagagttgg ataaggaaga aaatgaagca
     3661 ggaacacaaa gcgccgaagt gcaatcggat attagtttta gaaatgcagg aaccattggt
     3721 gttgtcagta aaggcggatt cggaatcaaa caaagcagtc agctcgctac ggaattattt
     3781 accgatttac gaataggaac gggtgcattg gtgatcagtt cttcgggagg atcggaatac
     3841 gcgatggaaa gtgcgcaatg gaaaaacgga ttgtttacct acagtctgct caatggaatt
     3901 gcttccggta aggctgatgt caaccatgat cagcaaatca cgattggtga actgcaggtg
     3961 tatgttgcag aacaagtgca atccttatcc ggaggagcac aacaacccgc cttccgaagg
     4021 gaaaaccttg agtttgatgt gagggtttgg tgatttttcc attcggtagt cttgttttcc
     4081 gaaaatgaat agggatgctg caggcaaatc tgttttttta tgtcggattt tttttgaatg
     4141 tttgactatg aacttggttc agcatccttc cggaatccaa tggtacaatg ccggtcaaat
     4201 cgcggtgcct cacgcttttt ttctgcgtca cggtggtgtt tctcctacgc cttgcgatgc
     4261 gttaaatttt agcggttcac tggatgagga agaaaacgta aaggaaaatc gccgacgtgc
     4321 atttgatgcg cttggttggg atatcgaaaa agccgcttac ctgaaacaga ttcacagcac
     4381 catcatatgc gatgcagaat ccggtcagca ggagggcgat gcgttggtga ccaacaaaaa
     4441 aggaattcct attgcggtga gcggagcaga ttgttttccc gtattgtttc acgatccggt
     4501 gatttctgta attgctgcgg cgcattgtgg ctggaaagga accatcaacc gactggcagg
     4561 aaaagtaatt cttcgcatgc aggaattgaa ttgcaaacct gaacatatac acgttgccat
     4621 cggaccggga atttcccgca tggcctacga agtcagtccc gagcttatcg atcagtttcg
     4681 tgaagcagga ttttcggatc atagtttttc cggcaggcac ctggacctgg cacaatgcat
     4741 ccgacaaaac ctgatggaat ccggtatttc tgaagctcat atccagacgc ttgctcgttg
     4801 caccaccgat gctgatttct tctcccaccg ccgcgataat ggccgtacag gccgtttatg
     4861 gtcagttatt tgtctctaac ccgacttttt ttcacccgaa acttgcaggt tgagcaaaaa
     4921 cccttatttt tgccgtccct aaatcattgg ttcggtagtt cagtcctgac taaacgcagg
     4981 ttttgagaaa catctaccaa ttagttcttt aaaaaattga ttgaataata aacttgatcc
     5041 acgcacctgc gtttgtcagg atccagacag gctttgcctc gtctggattg gttaggctca
     5101 tctttactta ttcagaaaat aatatcaggt tcggtagttc agtcctgact aaacgcaggt
     5161 tttgagaaac atctaccaat ta
//
LOCUS       DAOMYG010000224         5134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5609_length_5134_cov_9.480213, whole genome shotgun sequence.
ACCESSION   DAOMYG010000224 DAOMYG010000000
VERSION     DAOMYG010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5609_length_5134_cov_9.480213"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2651)
                     /locus_tag="PK610_08160"
     CDS             complement(<1..2651)
                     /locus_tag="PK610_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRJ35948.1"
                     /translation="MKKFRFIFLLLIAVAANAQIRIVPQTGHIGWFTMDVSPDGKRML
                     SAGPEGKMILWDLVSFKAIWYEYIHRAPINQVKWRMDNRYFLTACEDSTVTVWDTESM
                     KKIRTYHSKYPITSAAFNPKADLITYGDTKGNIFMFHLVSNEQILHFNVGSSVNDMVW
                     VDNGYVLFAGTEKHGVMGFEMDKGKKILDMKMTDAVTELQLSPNNKILIINLAGGSTE
                     IWNPSSGKGYGSMQNAVVKSKNGYKQFSRPAATFDGQFVLMYDKDHNLQIGRLDPVLT
                     QIYDAKMLQNEIRMIKASPANNSFLLADMDGGIWICFFTENDFNDQSRLYWHKMLFEP
                     ERIRNLYFKPNDESIAMKGSYEYEFNFKTGDLTRREDDSTALENTTTTMKFFSPSRMA
                     DGIYYAIHTTKDEMYKIKDQEYVEPVTMYALSKDTAVVAYIDNNQLNIYHIKEARFLK
                     QEKVNLKGYTLFNGHLNNQFVTLDKKGLRFIECNTLQEKTLKDAGKLQLAWLTASEDN
                     SIVHACDTKGIYYSWETASGKAIAAKGDLAKIKISQFEVDKNNSMMIATTFDQRLVRI
                     ELASGKITHELNSKLPPVIHLSINHKCDRVAIADLDGVVHIFDTQLQKEIISIIPSPT
                     NGLIAYTPDNYYIATKEAAQNVALVNGNSVLGFEQIDLRYNRPDKVLSSIGIADAGVI
                     ASYERAWKKRMERSGIMESTGNEVPTVELANFKSIPLTTDQTELELHVKGTSQKGIKA
                     VKIWVRGVPVLGRNGKEFASKDIKNSIDIKLKAPLIFGTNKIEIATVSTNGTESEKVE
                     FLITGKKEIPQDLYIVSIGVSKYNDNRYNLRYADKDATDLVGALKNTRAFANVHSELI
                     TNENVTREKVIATKELLKKSRPNDVVLV"
     gene            complement(2778..4055)
                     /locus_tag="PK610_08165"
     CDS             complement(2778..4055)
                     /locus_tag="PK610_08165"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ35949.1"
                     /translation="MKKILLLILIVFTGTISFSQTVCNPGGNVFIYSNYDGGELNIDV
                     DLNIPNIKIGVVGYEATRVNITGTFSGNVTEVIYAGYPSTNSTHCSVPVAATSVNGVS
                     SSIVSILSFPAATYSNPNGYNSIICNYSCNTTSSQGGCNTADQVAHYFLIQFSGSMYS
                     HLTQYGCWVSSTTYRISDGGNCCAQPAVAPVANFTASATTICEGECIDFTDNSTGTPT
                     SWTWTLTGAATTTSSTQNPSGICYLNAGSYTVTLTASNAQGTSTSTSSITVTAPPAIP
                     VINLSGNNLITSGGSSYQWNLNGTPISGATTASYAPTANGNYTVTITNASGCSSTSLP
                     FTYNSVSINETNATDWAMVFPVPATEFIHLKIAESMNCTWGSITDINGKIILKFPVNT
                     HQMQLDISSIPAGTYILTLTGSENVLTKTIQIQ"
     gene            complement(4158..>5134)
                     /gene="lhgO"
                     /locus_tag="PK610_08170"
     CDS             complement(4158..>5134)
                     /gene="lhgO"
                     /locus_tag="PK610_08170"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HRJ35950.1"
                     /translation="EHNIKHDVCGKIVVATDEKELPNFEKIFKTGLENKIEGIEKIGP
                     DKIREIEPHCVGIAGIWVPCTGIIDFRGATEKMVEIALSKNTESRLLLNEEVKSIDHH
                     ETHSVIHTSKGKHIANKLIFCGGLQADRLAKMDKVSIREKVVGFRGDYYELTEQGKHK
                     VKNLIYPVPNPDFPFLGVHFTRMTDGEIECGPNAVFTFKREGYGKTDFSLRDSWDALT
                     YIGTWRLFMANMKFGINEYRRAFSKRLFLKTLQRMVPSLTMDDIHPGRAGVRALLLAR
                     DGDTRDDFRIETAGNSIHVLNAPSPAATASLAIGGQIADMAEKQFGLN"
BASE COUNT         1293 a         1038 c         1102 g         1701 t
ORIGIN      
        1 accagcacaa catcgtttgg tcttgatttt ttaaggagct cttttgttgc aattactttt
       61 tcgcgggtta cattttcatt ggtgatcagt tcgctgtgaa cattggcaaa tgctcttgtg
      121 ttttttagtg caccaaccag atctgttgcg tctttgtcgg cataacgcag gttgtagcgg
      181 ttgtcgttgt acttacttac accaatactc acgatgtaca gatcctgagg aatttctttt
      241 tttccggtga ttagaaattc tactttttcc gattcggttc cgtttgtact tacggtcgct
      301 atttcaatct tgttggttcc aaaaataaga ggtgctttta gtttaatatc aatagaattt
      361 ttaatgtctt tcgacgcaaa ctcctttccg tttcttccaa gtacaggaac accgcgtacc
      421 cagattttta cggcttttat tcctttctgg ctggttcctt taacatgaag ttcaagttct
      481 gtttgatctg tcgtgagagg tatcgatttg aaatttgcca gttcaacagt gggcacttca
      541 tttcctgtgc tttccattat tcctgaacgc tccattcgtt ttttccaggc gcgttcatac
      601 gaagcgatta ctccggcatc tgcaattcca atggaagaga gaactttgtc gggacgattg
      661 tacctgagat cgatctgttc aaatccgagt acggaattgc cgtttacgag agccacattt
      721 tgcgcagctt ctttggtggc tatgtaataa ttgtcgggtg tataggcaat caatccgttt
      781 gtaggagaag gaatgatact gatgatttcc ttttgtaatt gcgtatcgaa aatatgcaca
      841 actccatcca gatctgcaat agctacacga tcacatttgt gattgatgct gagatgaatt
      901 acaggtggta gtttactgtt gagctcatgt gtgatttttc cgcttgcgag ttcaatacgc
      961 accagtcgct gatcaaatgt ggtagcaatc atcatgctgt tgtttttatc gacctcaaac
     1021 tgactgattt ttatttttgc aagatcacct tttgctgcga tcgcttttcc gcttgcggtt
     1081 tcccagctat aataaattcc tttcgtatca caggcatgaa cgatactgtt gtcttcgctt
     1141 gctgtaagcc atgccaattg taatttgcct gcgtctttca gcgttttttc ctgaagtgta
     1201 ttgcattcaa taaagcgcaa gccttttttg tcgagcgtta caaattgatt gttgaggtgt
     1261 ccgttgaaga gtgtatatcc tttcagattg actttctcct gttttagaaa acgcgcttcc
     1321 tttatatggt aaatgttgag ttgattgttg tcgatatatg ctactacagc agtgtctttg
     1381 ctcagggcat acatcgttac cggttcaaca tattcctgat ctttgatttt gtacatttca
     1441 tccttggtgg tatgaatggc atagtaaatt ccatctgcca ttcttgaagg tgaaaagaat
     1501 ttcattgtgg tggtggtatt ttccagcgct gtggaatcat cctctcttcg tgtaaggtca
     1561 cctgttttaa aattgaattc gtattcgtaa gaacccttca tggcaatgga ttcatcgttc
     1621 ggtttaaaat acaggtttct gattctctcc ggttcgaaca acatcttgtg ccaatagagt
     1681 cgggattgat cattaaagtc attttctgta aaaaaacaga tccagatacc tccatccatg
     1741 tcagcaagga gaaatgaatt gttggcagga ctcgccttga tcatacggat ctcattctgc
     1801 agcatttttg catcgtaaat ctgggtaagc acgggatcca atcttccgat ttgtaaattg
     1861 tgatctttgt cgtacatcag cacaaactga ccatcaaaag tggcagcagg acgtgaaaat
     1921 tgcttgtaac catttttgct ttttacgacg gcgttttgca tactgccgta tccctttcct
     1981 gatgaaggat tccagatttc agtagagcca ccggcaagat ttatgatcag aattttattg
     2041 ttcggactca attgaagttc ggttacagca tcggtcattt tcatgtccag tattttcttc
     2101 cccttatcca tttcaaatcc catcactcca tgcttttctg tgcctgcgaa tagaacatat
     2161 ccgttatcga cccaaaccat atcgtttacc gaggagccca cattaaaatg cagaatttgt
     2221 tcattgctca ccaaatggaa cataaaaata tttcccttgg tatcgccgta ggtaatcaga
     2281 tctgcttttg gattgaatgc tgcgctggta ataggatatt tgctatgata ggttctgatt
     2341 tttttcatcg attccgtatc ccaaacagtt acggtagaat cttcgcatgc tgttaaaaaa
     2401 taacggttat ccatacgcca tttaacctgg ttgattggtg cgcggtgaat gtattcatac
     2461 caaatggcct taaatgatac taggtcccat aaaatcattt tcccttcggg accggcactc
     2521 agcattcgtt ttccgtcagg cgaaacatcc atggtaaacc agccgatgtg tccggtttgc
     2581 ggtacaattc taatttgtgc attagctgca acagcaatca ataggagaaa aatgaaccgg
     2641 aattttttca tggggaattg ttgaatagcg agaagctaaa gatagtgatc cgtatttttt
     2701 accaactaag gtatttaccg taaaagaaaa cgggaagtat tagcttcccg tttttagatg
     2761 cagtggtttt tggcgggtta ttgtatctgg atggttttgg tcagaacatt ttcacttcct
     2821 gtcagcgtga ggatgtaggt gcctgcagga atggaactga tgtcaagttg catttgatgg
     2881 gtgttgacag ggaatttcag gattattttt ccgttgatat ccgtaatact accccaggtg
     2941 cagttcattg attctgcaat tttaaggtga ataaattcag ttgccggaac aggaaatacc
     3001 attgcccaat ctgttgcatt tgtttcgtta attgaaacag aattataggt gaatggcagt
     3061 gaagtggaag agcatcctga agcattggtg atggttaccg tgtagtttcc gtttgcagta
     3121 ggtgcatagg aagctgttgt tgctccggaa atgggtgttc cgttaagatt ccattgatag
     3181 ctggatcctc cgcttgtaat gaggttgttt cctgaaagat tgataaccgg aatcgcagga
     3241 ggtgcggtta ctgtaatact tgaggttgat gtgcttgttc cctgtgcatt gcttgcagtg
     3301 agggttaccg tatagcttcc tgcattgaga tagcaaattc cgcttggatt ttgtgtgctg
     3361 cttgttgttg tggctgcacc ggttaatgtc catgtccagg atgttggtgt tcctgtggaa
     3421 ttgtcagtga agtcgatgca ttctccttcg caaattgttg ttgcagaagc tgtgaaattg
     3481 gctacgggtg ctactgcggg ttgtgcacag caattgcctc cgtcggatat gcggtaggtg
     3541 gttgaactta cccaacagcc gtattgtgtt aaatgcgagt acatgcttcc gctaaattga
     3601 atgagaaaat aatgcgctac ctgatctgcc gtattacatc ctccctggct ggaggtggta
     3661 ttgcagctgt aattgcaaat gatactgttg taaccgttcg gattggaata agtggctgca
     3721 ggaaaactaa ggatgctcac aatggaagat gacacaccat ttacactggt tgctgcaaca
     3781 ggcacactac aatgggtact gttggtgctc ggatagccgg cataaattac ctccgtaaca
     3841 ttcccggaaa atgtaccggt aatatttacc cgtgttgctt catatccaac aacgccgatt
     3901 ttaatattgg gaatgttgag gtccacatca atgtttaatt cgcctccatc gtaattggag
     3961 tagataaata catttccacc tggattacaa accgtttgtg aaaatgaaat tgttccggta
     4021 aatacgataa gaatgagcag tagaattttt ttcataacga ggctttttag gtttttgaaa
     4081 ttgcgtataa caatgccaat gaagagaagc atcaatcgca cctaaaaatt aagaaaaaaa
     4141 tgattgacag ccaaaaatta attcagtccg aattgttttt cggccatatc tgcaatttgt
     4201 ccgccaatgg caagactggc agttgcagca ggggaggggg cgtttaagac gtgaatggaa
     4261 ttgcccgcag tttcaattct gaaatcatca cgtgtgtctc catctcgtgc cagtaacaat
     4321 gcccttactc cagctcttcc cgggtgaata tcgtccatgg ttaatgaagg aaccattcgc
     4381 tgcagggttt ttaagaacaa acgtttcgag aaagcgcgtc ggtattcatt gattccgaat
     4441 ttcatatttg ccataaacaa ccgccatgtt ccaatatacg tgagtgcatc ccaactgtcg
     4501 cgtaacgaga aatctgtttt gccgtagcct tcgcgtttaa atgtgaatac tgcattgggt
     4561 ccacattcta tttcgccatc tgtcattcgg gtaaaatgca ctcccagaaa tgggaaatcc
     4621 ggattgggta caggataaat gagattcttc actttgtgtt ttccctgttc agtgagttca
     4681 taataatcac ctctgaaacc taccaccttt tcccgaatac tgactttgtc catttttgca
     4741 agacgatcgg cttgtagtcc accgcaaaaa ataagtttgt ttgcaatgtg ttttcctttg
     4801 gaagtatgga ttacggaatg cgtttcatga tgatcaatcg atttcacttc ttcattcaga
     4861 agcaatcgcg attccgtatt ttttgagagt gcaatctcta ccattttctc cgttgctccc
     4921 cgaaaatcta taattcccgt acatggcacc catattccgg caatgcctac acaatgcggt
     4981 tcgatttcgc ggattttatc cggaccaatt ttttcaatcc cttcaatttt attctctaat
     5041 cctgttttaa agatcttttc aaaattgggc aattcttttt cgtcggtagc taccacaatt
     5101 tttccgcata catcgtgctt gatattgtgt tcgc
//
LOCUS       DAOMYG010000225         5124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5620_length_5124_cov_7.321957, whole genome shotgun sequence.
ACCESSION   DAOMYG010000225 DAOMYG010000000
VERSION     DAOMYG010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5620_length_5124_cov_7.321957"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PK610_08175"
     CDS             complement(<1..809)
                     /locus_tag="PK610_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRJ35951.1"
                     /translation="MERIYLDNAATTALDPEVLDAMLPLMRDDFGNPSSTHHFGRKTR
                     SAIETARKTIANLLNASPGEIIFTSGGTEADNTAIIRSVIDLGVRHIVSSPLEHHAVL
                     HTIEEAEKQYGVRVTWLDANSNGHLDLTQIEQSLKTNDKTLVSLMHANNEIGNLLPIQ
                     KVADMCKANNALFHSDTVQTMGHYSIDTKMIAADFLACGAHKFHGPKGAGFLFKRENL
                     KTHPFIQGGAQERGYRGGTENLYGIIGLAKAFEIAHRDMQKHREHVSGLKK"
     gene            890..2542
                     /locus_tag="PK610_08180"
     CDS             890..2542
                     /locus_tag="PK610_08180"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRJ35952.1"
                     /translation="MSATADKKQDLDFNKNEDQMKLLISGLNRKLDVIHQGGGTKRIE
                     KLHAEGKLTARERLDYLFDKDRETLEIGEFAGYEMYEEHGGCPAGGVVVKIGYVSGKQ
                     CIVVANDATVKAGAWFPITGKKNLRAQEIAMENRLPIIYLVDSAGVYLPMQDEIFPDK
                     EHFGRIFRNNAVMSSQGILQIAAVMGSCVAGGAYLPIMSDEAMIVDKTGSIFLAGSYL
                     VKAAIGEDIDNETLGGASTHCEISGVTDYKCKDDKDCLNRIRNIMSKVGDYEKAGFNR
                     EKPADPKKDPKEIYGILPESRNKPYDMREIIARLVDDSDFEEYKELYGKSILCGLARI
                     DGWAVGIVANQRMIVKSKKGEMQQGGVIYSDSADKAARFIMNCNQKKIPLVFLQDVTG
                     FMVGSRSEQGGIIKDGAKLVNAMSNSVVPKFTIVIGNSYGAGNYAMCGKAYDPRLIAG
                     WPTAQVAVMGGAQAAKVLVQIEVASLKAKGEQITPEREKELFDKIKDRYDRQTTPFYA
                     ASRLWLDAIIDPLDTRKWVSMGIEMANHSPITRPYNVGVIQT"
     gene            2637..3269
                     /locus_tag="PK610_08185"
     CDS             2637..3269
                     /locus_tag="PK610_08185"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRJ35953.1"
                     /translation="MSVLVGRKAPSFKASAVVNGNQVVNDFSLDQFLGKKHVLFFFYP
                     KDFTFVCPTELHAFQNKLAEFEKRNVAVVACSTDTEESHWGWLQMSKDQGGIKGVTYP
                     IVADTTKTISDDFGVLAGEYDYDDEGNLVASGPMIAYRGLFLIDKSGTVMHQLVNMFP
                     LGRNVDEALRMVDALQFFEENGEVCPADWHKGAEGMKATHDGVANYLAKH"
     gene            3364..3594
                     /locus_tag="PK610_08190"
     CDS             3364..3594
                     /locus_tag="PK610_08190"
                     /inference="COORDINATES: protein motif:HMM:NF026900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm17 family immunity protein"
                     /protein_id="HRJ35954.1"
                     /translation="MNNNMLALYLIYSTGLILIGAGVFNWELLTRSNGTEYMNSKFGR
                     NSGRILFVVLGLGILAMGYYAMRTGKFEGTIE"
     gene            3591..4349
                     /locus_tag="PK610_08195"
     CDS             3591..4349
                     /locus_tag="PK610_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRJ35955.1"
                     /translation="MRHGYCHIPLAPCRADASDKSEMVTQLLFGEHFEVLEKQEKWSK
                     IRIAQDAYECWIDNKQYLTLFVDQFEELQRKKILRTSENMGRIMHQGNVMFVPIGSAL
                     PFFDKGTIKISNQEYSYNGAYNGQQHIAEIARVFLNAPYLWGGKSSMGIDCSGFTQVV
                     FSVCGIQLPRDAYQQAEIGEVVDFMEEAQEGDLAFFDNEEGRIIHVGIILNDQRIIHA
                     SGKVRIDKIDHEGIYNEDSKSYTHKLRLIKRVGN"
     gene            4488..>5124
                     /locus_tag="PK610_08200"
     CDS             4488..>5124
                     /locus_tag="PK610_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRJ35956.1"
                     /translation="MDFNKFTIKSQEAVQQAQNLAMEKQNQQIEAGHLLKGIFLVDEN
                     VTPYLLKKLNINTVNLEKALDRIIDGYPKVSGGQEYLSRNLNSIIQKAIGLLKTSGDE
                     FVSIEHLLLAMLSVNDTASQLLKDAGVTEKDLNAAIAELRKGSKVTSASQEETYNSLN
                     KYAKNLNDLAKNGKLDPVIGRDDEIRRVLQILSRRTKNNPILIGEPGVGKTA"
BASE COUNT         1560 a         1008 c         1194 g         1362 t
ORIGIN      
        1 cttttcttaa gacctgaaac atgttcccta tgtttttgca tgtcacgatg tgcaatttca
       61 aaagcttttg cgagaccgat gataccgtac aagttttcag ttcctccgcg gtatccacgc
      121 tcttgcgctc caccttgaat gaaaggatgg gttttgaggt tctcacgctt aaacaaaaat
      181 ccagcgccct ttggaccatg aaatttatgt gcaccacaag cgagaaaatc tgcagcgatc
      241 atttttgtgt cgattgagta atgacccatc gtctgaaccg tatcggaatg aaaaagtgca
      301 ttattcgcct tgcacatatc cgcaactttt tgaatgggaa gtaaattccc tatttcattg
      361 ttggcatgca tcaggctaac taaagtttta tcgttggttt ttagactttg ctcaatttga
      421 gttaagtcaa gatgtccgtt tgaattggcg tccaaccagg taaccctgac gccgtattgt
      481 ttctcagctt cttcgatagt gtgcaaaacg gcatggtgtt ccagggggct ggaaacaatg
      541 tggcgcacac ctagatcaat aacagaacgg ataattgccg tattatcagc ttcagtgcca
      601 ccggaggtaa aaataatttc tccgggagaa gcattcagca agttggcaat ggttttacgg
      661 gcagtttcaa ttgcagaacg tgttttacgc ccaaaatggt gtgttgatga aggatttcca
      721 aaatcatccc gcataagcgg taacatagca tccagcacct caggatcgag ggctgtagtg
      781 gcggcattat caaggtagat cctttccata ttgcaaaaat aaggaaaaac aaacggtgca
      841 ggtcggtgaa gccttatatt tggagtccaa ttttaaccca aaagagatta tgtcggcaac
      901 agcggataag aaacaggacc tggatttcaa taagaacgag gatcagatga aacttttgat
      961 ttcgggtttg aaccggaaac ttgatgtcat tcatcaggga ggaggaacca agcgcataga
     1021 gaaattgcat gcagaaggga aacttacggc cagggagcga cttgattatt tgttcgacaa
     1081 ggacagagaa acactggaaa ttggagagtt tgccggctat gaaatgtatg aagagcacgg
     1141 agggtgtcct gcgggtggtg tggttgtcaa gattggatat gtatcgggca agcaatgtat
     1201 tgttgttgcc aacgatgcta ctgtaaaagc cggtgcctgg tttcccatta ccggaaagaa
     1261 aaatctgcgt gcccaggaaa tcgccatgga gaatcgttta cccataatct accttgtaga
     1321 ttctgcaggt gtatacctac caatgcaaga tgaaattttc cccgataagg aacatttcgg
     1381 gagaattttc cgcaacaatg ctgttatgag ttcccaaggt atactgcaga ttgccgctgt
     1441 aatgggaagc tgcgttgccg gaggagccta tctgcccatt atgagtgatg aagccatgat
     1501 tgttgacaaa acaggaagca ttttccttgc aggatcctat ttggtgaaag ccgctattgg
     1561 tgaggatatc gataatgaaa cgctgggtgg tgccagtaca cattgtgaga tcagtggagt
     1621 taccgattac aaatgcaagg atgataagga ctgcctgaat cgtatccgaa acatcatgag
     1681 taaagttggt gattatgaaa aagctggatt caaccgtgaa aaaccggctg atcctaaaaa
     1741 agatcccaag gagatttatg gcattttacc ggaatcgaga aacaagccat atgatatgcg
     1801 agagatcatt gcacgattgg tggatgattc ggattttgaa gaatacaaag agctttacgg
     1861 aaaaagtata ttgtgcggat tagcccgaat tgacggctgg gctgtgggta ttgttgctaa
     1921 tcagcgaatg attgtaaaaa gcaagaaagg agaaatgcag caaggtggag tcatttacag
     1981 cgacagcgcc gataaggctg cacgatttat catgaattgt aaccagaaaa aaattccgtt
     2041 ggtgtttttg caggacgtta caggtttcat ggtaggctca cgatctgaac agggtggaat
     2101 cattaaggac ggtgccaaac tggtaaacgc catgagtaat tctgttgttc ccaaattcac
     2161 cattgtgatc ggtaattcat acggtgcagg aaattatgcc atgtgcggta aagcctatga
     2221 tccgcgtctg attgcaggct ggccaacagc acaggtggca gttatgggag gtgcacaggc
     2281 agcaaaggtg cttgttcaga ttgaagttgc ttctctcaag gcgaaaggag aacaaatcac
     2341 accggaaagg gaaaaagaat tgttcgataa aattaaagac cgttacgaca ggcaaaccac
     2401 tccgttttat gcggcatcac gactttggct ggatgccatt atcgatccat tggatacccg
     2461 caagtgggtt tcgatgggca tcgagatggc caaccactcc cctattacac gcccttataa
     2521 tgtgggagtt atccagacct gaagagcttg ttcaaaacaa attcggggat cattggttat
     2581 tgtatcaaac acagggctaa ttttgcactc attcacaaac taaaaaacaa aaaaatatgt
     2641 cagtacttgt aggaagaaaa gcaccatcat tcaaagcatc agccgtagtt aacggaaacc
     2701 aggtggtgaa tgatttttct ttggatcagt tccttggtaa gaagcacgtt ctgttcttct
     2761 tctaccctaa ggatttcacg ttcgtttgtc ctaccgagct tcacgctttt cagaacaaac
     2821 ttgcagaatt tgaaaaaaga aatgttgcag tagttgcctg ttcaaccgat actgaagaat
     2881 cgcattgggg atggcttcag atgagcaaag atcagggcgg aattaaaggt gtaacttatc
     2941 cgatagttgc ggatactacc aaaaccattt ctgatgattt tggagtattg gcaggtgaat
     3001 acgattatga tgatgaagga aacctcgtgg catccggtcc aatgatcgct taccgcggtt
     3061 tattccttat cgacaaatca ggtaccgtta tgcaccagct ggtaaatatg ttcccgctcg
     3121 ggcgtaacgt tgatgaggca ctccgaatgg tagacgctct ccagtttttc gaagaaaacg
     3181 gagaagtttg tcctgccgac tggcataaag gtgctgaagg aatgaaagcc acgcatgatg
     3241 gtgttgcaaa ttaccttgcg aaacactaat ctgttcaaaa cttacaatga aaagaccggt
     3301 catttgatcg gtctttttca tttttaaccc ggtttaggaa aaaggaaaat gtaaatttgc
     3361 accatgaaca acaacatgct tgcgctgtat ttaatttatt caacaggatt gattctgatt
     3421 ggcgcaggtg ttttcaactg ggaattgctc acccgttcga acggtactga gtacatgaat
     3481 agtaaattcg gaagaaacag tggtcgtatt ttatttgtag ttcttggtct tggcattcta
     3541 gcaatgggat actacgccat gagaaccgga aaatttgaag gcacaataga atgagacacg
     3601 gttattgcca tattcctctt gcgccctgtc gtgctgatgc ctccgataaa agtgagatgg
     3661 ttactcagct cctattcgga gagcactttg aagtgctgga aaagcaggaa aaatggagca
     3721 agattcgtat tgcccaagat gcatatgaat gttggattga taacaagcaa tacctcacac
     3781 tatttgttga ccaatttgaa gaactgcagc gaaaaaaaat attacgtacc tcagaaaaca
     3841 tgggtagaat catgcatcag ggtaacgtta tgtttgtccc cattggttct gcacttccct
     3901 ttttcgataa aggcacaatt aaaataagca accaggaata cagttacaat ggtgcatata
     3961 atggtcagca acacattgca gaaattgccc gtgttttttt aaatgcaccc tatttatggg
     4021 gtggaaaaag cagcatggga atcgattgtt cgggctttac ccaggtcgtc ttttccgtat
     4081 gcggaattca actgcccaga gacgcttacc agcaggccga aattggagag gtggttgatt
     4141 tcatggagga agcccaagaa ggagaccttg cttttttcga caatgaagaa ggaagaatta
     4201 ttcatgtagg aattattctg aacgatcagc gcatcattca tgcttccgga aaagtccgca
     4261 tcgataaaat cgatcatgaa ggaatttata atgaggattc aaaaagctac acgcataagc
     4321 ttagactcat aaaacgcgtt ggaaactgac attttaccct tttttttttc cagcacaaga
     4381 cattatttca tctattccaa gcttaaacac gccattattt ccgctttttt cgggaaatgg
     4441 ccattggttt acttcttgat gcagattaat ctgtaaaaac aacaatcatg gatttcaaca
     4501 aattcaccat caagtcgcag gaggcagtgc agcaggctca gaatctggca atggaaaaac
     4561 aaaaccagca aattgaagca ggacacttgc taaagggaat ttttctcgtt gatgaaaacg
     4621 taactcccta cctgctgaaa aaactcaaca ttaacacggt taatcttgag aaagcattgg
     4681 atcgcatcat tgacggttac cctaaagtga gtggcggaca ggaatacctt tcgcgcaacc
     4741 tgaacagtat aattcagaaa gcaattggtt tattgaaaac atccggcgac gaatttgtgt
     4801 cgatagaaca cctactcctc gcgatgttat ctgtgaacga taccgcttct caattgctga
     4861 aggatgccgg agtaaccgaa aaagacctta atgccgccat tgcagaacta cgaaaaggaa
     4921 gtaaagtcac cagcgcatcg caggaagaaa cctacaattc cttaaacaaa tacgccaaga
     4981 atttaaatga tcttgccaaa aacggaaaat tagatccggt tattggtcgc gatgacgaga
     5041 ttcgccgcgt attgcaaatt ctttcacgca gaacgaaaaa caatccaatt ctcattggag
     5101 aaccaggtgt tggtaaaaca gcta
//
LOCUS       DAOMYG010000226         5114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5640_length_5114_cov_7.892074, whole genome shotgun sequence.
ACCESSION   DAOMYG010000226 DAOMYG010000000
VERSION     DAOMYG010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5640_length_5114_cov_7.892074"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      <1..103
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            159..2612
                     /locus_tag="PK610_08205"
     CDS             159..2612
                     /locus_tag="PK610_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ35957.1"
                     /translation="MKKWMILLLGVLFVSTLKAQSGISGTVKNESGEPLSGVNVKVVN
                     SLLGAVTDSEGKFELIKIPSGDYKIRFTMIGYESQEHSVKVESGIQQLEIRLKFAVNV
                     LEEITVEASRTNNDKGIAFTDVSKEDIAKNNLGPDIPYLLDQTPSVVVTSDAGTGIGY
                     TGIRIRGSDPTRTNLTINGIPLNDAESQGTFLVNLPDFASSVDNIQIQRGVGTSVNGA
                     GAFGASINLSTNTYSEKAYGELSNSFGSFNTRRHTLKAGTGLINGKFTFDMRLSSIES
                     DGYIDRAFSNLKSFYTSSAWYGKNSSLRLNIFSGKEHTYQAWAGIPYDSLFTNRRYNP
                     YTYDNESDNYTQTHYQLFFNHRFGKKLIANIALHYTRGNGYYEQYKEDRSFSSINQPD
                     LVIGGDTISKTDWIQRRWLDNHFAGTVFSFIYEASDKLQLVFGGGSNIYSGKHFGEII
                     WAEYAVVPVRYRYYDNNANKTDMNVYLKADYLINDKWNVYLDLQQRLVDYTFLGTDEY
                     GMPLKQTVSHSFFNPKLGTVYRLNKQSSVYASFGVGNKEPNRDDYVSNPPNSRPLHET
                     LFDHEAGYRLKKQKMNVGVNFFYMDYYNQLAPNGKINDVGAVVRTNIDRSYRMGVELD
                     GAVQATKRVLIKANLTLSQNRAVEFTEYIDNWDTWGQDTIVYRNTDLAFSPSVVSGQE
                     ISFNLFRWEDTKNNEVEKEKQSGRIDFSLIGKYVGRQMLDNTGRNDAKVDDLTATDLK
                     FRSLDAYFILDARLTFRMKIPSVAEIDLNIMLRNVLDEMYSSNGWTYKYSLGGEYSAM
                     SGFFPQAGRNFLAGLTIRF"
     gene            complement(2620..3240)
                     /locus_tag="PK610_08210"
     CDS             complement(2620..3240)
                     /locus_tag="PK610_08210"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRJ35958.1"
                     /translation="MELIVVSSPDKFKSEIPIVRDLFAAGLTTLHIRKTKFSTAKLRE
                     YLEEVPEKYHSRIIIHTHHQLAYHYHLKGIHFTKHHRKNKAKLFFKRTMFHLRKPTML
                     MTRSFHQIETMRTDRHLYSYAFLNPYFSRIEPMKNSFDISNDFLAKLIKESKSPVFAS
                     GNIDLNNYHLLQNMNLQGFALSKAIWKYQGDKAALFQKISSEVASW"
     gene            complement(3242..3970)
                     /locus_tag="PK610_08215"
     CDS             complement(3242..3970)
                     /locus_tag="PK610_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HRJ35959.1"
                     /translation="MNLFDFFSNQHKNKKKLLGVLLDPDKAPEGEQLKTFILNCEKAG
                     VDIFLAGGSLITKGNLDLLIQSMKQFTKLPVLIFPGDSSQISNEADGILLLSLISGRN
                     PEYLIGQHVASASRLKQSKLQILPTGYLLIDSGTVTTAQYVSNTVPLPSEKPEIAANT
                     ALAGELLGMKLIYMDAGSGAKRCITEECIMAVRNTISIPLIVGGGINSIEKANAAWNA
                     GADMVVVGTLFEKQPEAITLFSKR"
     gene            complement(3976..4596)
                     /locus_tag="PK610_08220"
     CDS             complement(3976..4596)
                     /locus_tag="PK610_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HRJ35960.1"
                     /translation="MGLIANIVPNKASGIWIWKIIEEEKQLGLGLPDDILTAAGQFKA
                     ESRRKEWLAVRQILQIAAGKTELIYDPSGKPSLPGLYLSVSHTTGYSGVYLSTEYQGG
                     LDLEAISPRILKIAERFLNEQEKKRFSTDDIQLLTLLWSTKEALFKKHGGETTNFALN
                     QDILSVDQKTQEIKAVVKINNNTIEESLAFQIYEEVVMVYTQNKTS"
     gene            4680..>5114
                     /locus_tag="PK610_08225"
     CDS             4680..>5114
                     /locus_tag="PK610_08225"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRJ35961.1"
                     /translation="MSALFALVFAFNSVAQVLFPISVNHKDGYIDKSGKVIVEPKYDK
                     TFPFRNGLGRFQEEGKTGFLNASGKVVIEAQFDRATDFNDGLAAVSNGGKWSFIDEKG
                     AVKFEVNCNTAFPFSDGMARFQLSGKMGFVDKTGRVVVEPRYE"
BASE COUNT         1476 a          959 c         1089 g         1590 t
ORIGIN      
        1 gtgccttact atcaagggct gagatcatac ccgtttcccg tttgcttttc gggaagaacc
       61 tgatcagggt aatgcctgcg gagggatgaa acaaaagcac agtgtattta agttgttttc
      121 ccgatgtgtc tcagcaaagg gcaaataatg ttaacgccat gaaaaaatgg atgattttgc
      181 tattgggtgt gttgttcgtt tcaacgctca aagcacaaag cggtatttca ggtaccgtaa
      241 aaaatgaatc cggtgaacca ctttccggag taaatgtaaa agtggtgaat tcattattag
      301 gtgcggttac ggattcggaa ggaaaatttg aattgattaa gattccttca ggggattata
      361 agatccgatt cacaatgatt ggttacgaat cgcaggaaca ttcagtgaag gttgaatccg
      421 gcattcagca attggagatc agattaaaat ttgcggtcaa tgtcctagag gaaattacag
      481 ttgaagcctc acgtaccaat aacgataaag gaattgcatt taccgatgta agtaaagagg
      541 acatcgcaaa aaataattta ggaccagata tcccttatct tttggatcaa actccttcgg
      601 tggttgttac ttccgatgcc gggaccggga ttggttatac aggaattcgc atccgcggat
      661 cggatccaac acgaacaaat cttaccatta atggaattcc tttgaatgat gcagaatccc
      721 aaggtacatt tctcgtaaat ctacctgact ttgcgagttc agtagataat attcagattc
      781 aacgtggtgt agggacctcc gtaaatggtg caggtgcatt tggcgcaagc attaatcttt
      841 ctaccaatac ctacagcgaa aaagcatatg gagaattgag taattcattt ggttcattca
      901 atacccggcg tcatacttta aaagccggaa caggattgat aaatggcaag ttcacgtttg
      961 atatgcgact atcttccatt gaatcggatg gatatatcga cagagcattt tccaatttaa
     1021 aatcctttta cacttcatcg gcatggtacg gaaagaacag ctcgctaaga ctgaatatct
     1081 tttccgggaa agaacacact taccaggcat gggcaggaat tccatacgac tccttattta
     1141 ccaaccgtcg ttataatccg tatacctacg ataatgaatc ggataattat acccaaacgc
     1201 attatcagtt gtttttcaat catcgctttg gaaaaaaatt aattgcaaac attgctttgc
     1261 attatacacg agggaatggt tattatgagc aatacaaaga agatcgttca ttctcatcga
     1321 tcaaccaacc cgatttggtt attggaggtg acactatttc aaaaactgac tggattcaac
     1381 gcagatggtt ggataaccat tttgccggaa ccgtgtttag ttttatttat gaggcatcag
     1441 ataaattgca attggtgttc ggaggaggat cgaatattta ctccggaaaa cattttggag
     1501 aaataatctg ggctgaatat gcagttgttc ctgttcgcta tcgttattac gataacaacg
     1561 ccaacaaaac cgatatgaac gtttatctca aagctgatta tttgataaat gataaatgga
     1621 acgtgtactt agatctccaa caacgtctgg tggattatac atttctcggt acagatgaat
     1681 atggaatgcc gttaaaacaa accgtaagtc attctttttt taatcccaaa cttggcaccg
     1741 tttatcgttt gaataaacaa agctcagttt atgcatcttt tggtgttgga aataaagaac
     1801 caaatcgcga cgattacgtc tccaaccctc caaactcccg accattacat gaaacactct
     1861 ttgatcacga agccggatat cggctaaaaa agcagaagat gaatgtggga gtcaatttct
     1921 tctatatgga ttattataac cagctggctc caaatggtaa aatcaatgat gttggtgctg
     1981 tagtgcgtac aaacattgat cgcagttaca ggatgggcgt tgagcttgat ggagcagttc
     2041 aggcaacaaa acgggtgctt ataaaagcca atcttacatt aagtcaaaat cgggcggtag
     2101 aattcacgga gtatatcgac aactgggata cctggggaca agacactatc gtttatcgaa
     2161 atacagatct tgcattttct ccttctgttg tttccgggca ggaaattagt tttaatcttt
     2221 tccgatggga ggataccaag aacaatgaag ttgaaaagga gaaacaatca ggtagaatcg
     2281 atttttcgct gattggaaaa tatgttggcc gtcagatgct cgataatacc ggaagaaatg
     2341 atgcaaaggt tgacgatctg acagcaaccg atttaaaatt cagaagtctt gatgcctatt
     2401 ttattttgga tgcacgcctc acttttcgaa tgaagatacc ctctgttgcg gaaattgatc
     2461 tgaatataat gttgcgaaat gtgcttgatg aaatgtattc ttcaaacggc tggacctaca
     2521 aatattctct cggaggtgag tacagtgcga tgagtggatt ttttccacag gcaggacgaa
     2581 attttctggc cggattaacg attcggtttt aataatgatt taccatgatg cgacttcgct
     2641 gctgattttt tgaaataagg cagctttgtc gccttggtat ttccagatcg ctttggataa
     2701 tgcaaatcct tgcagattca tgttttgcaa aagatgataa ttattcagat caatattgcc
     2761 gcttgcaaaa acaggtgatt ttgattcttt gattaattta gcaagaaaat cattactgat
     2821 atcgaatgag tttttcattg gttcaatccg tgaaaaatac ggattcagaa atgcatacga
     2881 gtaaaggtgt cgatccgttc tcattgtttc tatctggtga aaagaacggg tcataagcat
     2941 cgttggtttt cttaaatgaa acattgtacg cttgaaaaat aattttgctt tatttttccg
     3001 gtgatgcttg gtgaaatgaa tacctttcag gtgataatgg tatgcaagct gatgatgggt
     3061 atggataatg atccgcgaat gatatttttc aggtacctcc tcgaggtatt cacgaagttt
     3121 tgctgtgctg aatttcgttt ttcttatatg caatgtcgtt aatcctgcag caaagagatc
     3181 gcgcacaatt gggatttctg atttgaattt gtccggactg ctaacaacga tgagttccat
     3241 tttatcgctt ggaaaataat gtaattgctt ccggttgttt ttcaaataga gtgcctacaa
     3301 caaccatatc agcaccggca ttccatgctg catttgcttt ttcaatactg ttaattccac
     3361 ctccaactat cagcggaatg gagattgtat ttcgtacagc cataatgcat tcttcggtaa
     3421 tgcatcgttt tgctccgcta cctgcatcca tatagatgag cttcatgcct aaaagttctc
     3481 cggctaatgc tgtgtttgca gctatttcag gtttctcaga aggaagaggc actgtgttgc
     3541 ttacgtattg cgcagtagtt actgttccac tatctatcag taaatatcct gtagggagaa
     3601 tttgaagttt cgattgtttc aagcgagagg ctgaggcaac atgttgtcca atcagatatt
     3661 caggatttct tcctgatatg agcgacaaaa gcaaaatgcc gtctgcttca ttggaaattt
     3721 gtgatgagtc tccgggaaat attaatacag gaagtttggt gaattgcttc atggattgaa
     3781 tcagtaaatc caaattccct ttcgtaatca aacttccacc cgcaagaaaa atatctaccc
     3841 cggctttttc acaattcagt ataaaagttt tcaattgctc tccttccggt gctttatccg
     3901 gatcgagtaa taccccaagg agtttcttct tgtttttatg ttgattggaa aagaaatcaa
     3961 aaaggttcat cttcttcagc ttgtcttgtt ctgtgtatat accatcacca cttcctcata
     4021 gatctgaaaa gcgagggact cttcaattgt gttgttgtta atttttacta ctgctttaat
     4081 ctcttgtgtt ttttgatcta ctgaaagaat gtcctggtta agggcaaaat tggtggtctc
     4141 ccctccgtgc ttcttaaata gcgcttcttt tgtcgaccag agcaaagtta acagttgaat
     4201 gtcatctgta ctaaaccgtt ttttttcttg ctcgtttaaa aatcgctctg caatttttaa
     4261 gattctggga gaaatggctt caagatccag gcctccctga tattcggtac tgaggtaaac
     4321 gcccgagtag cctgtagtat gcgaaacaga gaggtaaaga cctggtaaag agggtttgcc
     4381 ggaggggtcg taaattaatt ccgtctttcc tgctgcaatt tgaaggatct gacgaaccgc
     4441 cagccattct ttcctgcgcg attctgcctt gaattggccc gcagcggtaa ggatatcatc
     4501 gggaagtcca agacctaatt gcttttcttc ttcgatgatt ttccaaatcc agatacctga
     4561 agccttattt ggtactatat ttgcaatcag acccacagtg ttttaattca aattttatta
     4621 acttagtaca tcagttttta cccttcgtta cagatatgcg tccaatttca aaatatttct
     4681 tgtcagcact ttttgcactc gtcttcgcgt tcaactcagt tgcacaggtt ctttttccaa
     4741 tttcagtgaa ccacaaagat ggctatatcg acaaaagcgg caaagtgatt gttgagccca
     4801 aatatgacaa gacatttcca ttccgcaatg gtctgggtcg ttttcaggag gaaggcaaga
     4861 caggtttttt aaatgcttcc ggaaaggttg ttattgaagc tcagtttgac cgtgccaccg
     4921 attttaatga tggattggca gcagtaagca atggtggaaa atggtccttc atagatgaaa
     4981 agggagcggt taagtttgaa gtgaactgca atactgcatt tcctttctct gatggtatgg
     5041 cgcgttttca gctcagtgga aaaatgggtt ttgtggataa aacgggtcgg gtagtggtag
     5101 agccgcgtta tgaa
//
LOCUS       DAOMYG010000227         5110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5646_length_5110_cov_7.549951, whole genome shotgun sequence.
ACCESSION   DAOMYG010000227 DAOMYG010000000
VERSION     DAOMYG010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5646_length_5110_cov_7.549951"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1812)
                     /locus_tag="PK610_08230"
     CDS             complement(<1..1812)
                     /locus_tag="PK610_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35962.1"
                     /translation="MSRVVGISLEWVLVFIIFLVFAIKSSAFQTWLAQRIAGYLSNEL
                     NAEVSIGKVDIDFFDYATLEDVLVRDLRGDTVVFAPKIHCDISYLSIREEKLRLSKVE
                     LVNPHLELKKYEGDKVWNYDFLEEYFSSDKPKRRDTTTKPWDVYVDRIMLTNSHLDYH
                     NFNKPWQDFGVDFDHMAVRGLNASMSDFHKSGDTAVVHMDRLFLSENSGFLLDTLAAT
                     IKIHENYLGFDELLIATANTRLRSPRLSFFFSDIDDFDDFEEKVKMEAQILPSKVYMK
                     DISFFAPELKGFDRTVQLSGKVRGTVNELKIRQLDLALSDQTYIRGNIDLIQVTDFEN
                     VFIDAKIKEFSGTMAELATIPIPPFDSATIDFPPRFSKIGKFNGRGSFTGGTNDFVAY
                     TEVNTDVGSVKTDIRFYVNPKDGFFHYDGDLITNEFKAGYFYDMPELGNVSMKIDIKA
                     TGITVENLYADIQGDIFSLGFEGYNYSNIRLAKGKLSPDRFVGKLEVKDPNLKMNFDG
                     SIVFGDKEPVFNFNLDVREAHPVQLNLSKRDPSASLCFSVTADAIGSDIDVFEGRLSV
                     TSVDYYEKGKEYHVDSIYLAAEQLPEKRKALVVRSDVM"
     gene            1861..2895
                     /gene="tsaD"
                     /locus_tag="PK610_08235"
     CDS             1861..2895
                     /gene="tsaD"
                     /locus_tag="PK610_08235"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008991875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRJ35963.1"
                     /translation="MLINKDTTILGIESSCDETSAAVLKGGILLSNVIAGQKVHEQYG
                     GVVPELASRAHQQNIIPVVHQAIQQAGINKQDIDCVAFTRGPGLLGALLVGTSFAKSF
                     ALARNLPMVEVNHMQAHILAHFIRKPGQDESTMPQFPFICLTVSGGHTQLVLVKSPLQ
                     MEIIGETIDDAAGEAFDKAAKMLGLPYPGGPLIDKLAKEGNPEKFRFSEPHLPDLQYS
                     FSGLKTSILYFLRDAVKTDPAFIENNKADLCASIQHTIISILLKKLSKAIDIYKVKDI
                     AIAGGVSANSGLRNTLLQKQDEWNVKVHIPPFEYCTDNAAMIAMAGAFRHAEGLFASQ
                     NIAPLARMSF"
     gene            2986..3594
                     /locus_tag="PK610_08240"
     CDS             2986..3594
                     /locus_tag="PK610_08240"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRJ35964.1"
                     /translation="MSFELAPLPYANNALEPFIDAMTMEIHHDRHHAAYVNNLNAAIK
                     GTDLEGKSLLEIITNISKAPMAVRNNGGGHFNHDLFWNIMAPNKGGEPTGALADAIKS
                     TFGSFEDFKTQFNAAGATRFGSGWAWLIVKSDKSIAICSTPNQDNPLMDIAEVKGTPI
                     LGCDVWEHAYYLKYQNKRPDYMANWWNVVNWDAVADRYKAAL"
     gene            complement(3663..5054)
                     /locus_tag="PK610_08245"
     CDS             complement(3663..5054)
                     /locus_tag="PK610_08245"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HRJ35965.1"
                     /translation="MFSQFRITALLGFSVLLLLNSCKTSVKPEKPDESYVPVKTEINR
                     EISFINLPMEIALSDIQKQINAYVKGVIYEDNSFTDNDNDGLKVKVKKHEDIKVEGLQ
                     NKIRLTLPLSISGQYKTLGVTTSFNGILKTTYVSAITLKDNWKVETITKSAGHEWIKS
                     PAIDLYLFELPVTMIADAALEGQKEFIEKEIDKAIREYVDLKDYVGDVIRGLYQPMLV
                     SETYKTWFCLEPIEIYASQINTQKGIMKVSLGMKTYTETHIGPKPVVDSSRFPIMKIR
                     EDLPDDFHVGLVNMIKYPHAAALMKEQYVDNPYTYTEGKRSVTLTGFDMWGQKDKMVV
                     EVGLKGSVNGNIYLMGIPAYDSVSRNIVMRNVDFHMDTKNKLLKSANWLLHGKFAKVM
                     EKNMYFEIGKQLDQAKKDCQTYLDNYEISKGIVLKGKLNDISTRNVFLANEAIVTLVS
                     ANGKLSIRVEGME"
BASE COUNT         1520 a         1109 c          980 g         1501 t
ORIGIN      
        1 cataacatcc gatcgaacaa caagcgcctt tctcttttca ggcaactgtt ctgcggccag
       61 gtaaatggaa tcgacatggt attcttttcc cttttcgtaa taatctacac tggttaccga
      121 aagtcttcct tcaaatacat cgatatcact tccaattgca tcggcagtga cagaaaaaca
      181 caagctggca gaaggatcgc gctttgataa attcaattga acgggatggg cttcacgaac
      241 atcaagattg aaattaaaca ctggttcctt gtcgccaaac acaatacttc cgtcaaaatt
      301 cattttaaga tttggatctt ttacttccaa tttcccaacg aagcggtcgg gacttaactt
      361 cccttttgca agtcggatat tggagtaatt atatccttca aatcccagcg aaaaaatatc
      421 accctgtata tctgcatata gattctcaac agttataccg gttgctttaa tatcgatttt
      481 catactgaca ttacccaatt ccggcatatc ataaaaatag ccggccttga attcgttcgt
      541 gataaggtca ccatcgtaat ggaagaatcc atcttttgga ttaacataaa aacggatatc
      601 ggttttaaca gatcctacat cggtattcac ttcggtatac gcaacaaaat cgttggtacc
      661 tccggtaaaa gagcctctgc cattgaattt tccgattttg gaaaaccgtg gagggaaatc
      721 gatggtagcg ctatcgaacg gtggaatcgg aatggttgcc aattcagcca ttgtacctga
      781 aaattctttg attttggcat cgataaacac attttcaaaa tcagtaacct gaatcagatc
      841 aatattgcct ctgatgtagg tctgatcgct tagtgcaaga tcgagctgac ggattttcaa
      901 ttcattcaca gttcccctta cttttccgct cagctgcaca gttctgtcaa atcctttcag
      961 ttcaggagca aaaaaggaaa tatccttcat gtaaactttg cttggaagaa tttgggcttc
     1021 cattttcact ttctcttcaa aatcatcgaa atcatcaata tccgagaaga aaaacgataa
     1081 acgtggagag cgcaaacggg tattggctgt agcgattaac agttcatcaa aaccaagata
     1141 gttttcatga attttaatgg tcgcagcaag cgtatcgagt aaaaatccgc tattctcaga
     1201 gagaaacagg cgatccatat gcacaacagc agtatcaccg gatttatgaa aatcactcat
     1261 gctggcattc aatccccgaa cagccatatg atcgaaatca acaccgaaat cctgccacgg
     1321 tttattgaaa ttgtgataat cgagatggga attggtgagc attatccgat cgacatacac
     1381 atcccagggc ttggtagtgg tatcgcggcg tttgggtttg tccgaactaa aatattcttc
     1441 aaggaaatcg taattccaaa ccttatcgcc ctcatatttt ttcagttcga gatgcggatt
     1501 gaccaattct actttggaaa gcctgagttt ttcttcccgg atagagaggt aactgatatc
     1561 gcaatgaatt tttggagcga aaaccactgt atcaccacga agatcgcgga caagcacatc
     1621 ctcgagtgtg gcgtaatcaa aaaaatcgat atcgaccttg ccgatactga cctcagcatt
     1681 aagctcgttg gataaatacc cggcaattcg ctgagccagc caggtttgaa aagcggatga
     1741 ttttatggca aaaacaagga aaataataaa aacaagcacc cactccagcg agatacctac
     1801 tacacgcgac aatatcctga ttactttttt gatagttttg cagtgctgat ttctacgaac
     1861 ttgttaatca acaaagatac cacaattctc ggcatcgagt cgagctgcga cgagacctct
     1921 gcggcggtgt tgaagggagg gattttgctc tctaacgtaa ttgcaggcca gaaagtacac
     1981 gagcaatacg gcggtgtagt acctgaactt gcatccagag cgcatcagca aaacatcatt
     2041 ccggttgtgc atcaggctat tcaacaggca ggaatcaaca agcaggacat tgattgtgtt
     2101 gcgtttacaa gagggccggg actattgggg gcattattag taggtacctc ttttgcaaaa
     2161 tcttttgcgc tggcgaggaa tttacccatg gtggaagtga accatatgca agcccatatt
     2221 ctggcacact tcatccgcaa accgggtcaa gatgaaagta ctatgcccca atttccattt
     2281 atctgcctga ctgtttcggg aggtcacact caattggttc tggtaaaatc acctttacag
     2341 atggaaatta tcggagaaac cattgacgat gctgccggtg aggcttttga taaggctgca
     2401 aaaatgctgg gactccctta tcccggcgga ccattgatcg ataagcttgc aaaagaaggg
     2461 aatccggaaa aattcagatt ttcagagccc cacttacccg atcttcaata cagtttcagc
     2521 ggattaaaaa caagcatact ctatttcctt cgggatgcag taaaaacgga tcctgcattt
     2581 attgaaaaca ataaggcgga tttatgcgct tccattcagc acaccattat ttctattctg
     2641 ctaaaaaaac tgagtaaagc aatagatatc tacaaggtaa aagatattgc aattgccgga
     2701 ggagtaagtg caaattcagg actacgcaat accttgcttc agaaacaaga tgaatggaat
     2761 gttaaagttc acattcctcc atttgaatat tgcaccgata atgccgcaat gattgcaatg
     2821 gcgggtgctt tcaggcatgc ggaaggactc tttgcatcgc aaaacattgc tcccctggca
     2881 agaatgagtt tctgaattgt agagaaagta gagaaagatc tctccatttt tactgttgtc
     2941 attaaccaaa tcaatttaca tttgttgtac caaaaacaat caattatgtc atttgaacta
     3001 gcacctctac cctacgcaaa caatgcgctg gaacccttta tcgacgcaat gaccatggaa
     3061 attcaccatg atcgtcacca cgcagcatac gttaacaacc ttaacgctgc aattaagggc
     3121 accgacctcg aaggaaaatc ccttcttgag atcatcacca atatttcaaa agccccaatg
     3181 gcagtaagaa acaatggtgg tggacatttt aaccatgatc ttttctggaa cattatggct
     3241 ccgaacaaag gaggagaacc aactggcgca cttgctgatg caattaaaag cacattcggt
     3301 tcttttgaag atttcaaaac ccaattcaac gctgccggag ctacacgttt cggttctggt
     3361 tgggcctggt taattgtgaa atcagataaa tcaattgcta tttgctctac acctaatcag
     3421 gacaatccat taatggacat tgccgaagtt aagggaacac ctattttagg atgtgatgta
     3481 tgggaacacg cctattatct gaagtatcag aacaagcgtc cggattacat ggctaactgg
     3541 tggaatgttg ttaactggga tgccgttgct gatcgttaca aagcagcact ataattaagg
     3601 atttcaaaaa aaaaaccgac agagaattct gtcggttttt tttttgcttt ttattcaagc
     3661 aattactcca ttccttctac ccgaatggaa agttttccgt tggcagaaac aagggttaca
     3721 attgcttcgt ttgcaagaaa tacatttctc gtactaatat cattcaattt tcctttcaga
     3781 acgattcctt ttgaaatctc ataattatcc agataagtct gacaatcttt tttagcctga
     3841 tcgagttgtt ttccgatttc gaaatacatg ttcttctcca tgacctttgc aaactttccg
     3901 tgcaacaacc agttggccga ttttaaaagt ttgtttttgg tatccatatg aaaatcgaca
     3961 ttacgcatta cgatgttcct gctcacactg tcataggcag ggattcccat caggtaaata
     4021 tttccgttca cgctgccttt cagtccaacc tctactacca ttttatcttt ttgtccccac
     4081 atatcgaatc ccgtaagcgt aactgatcgt ttaccctcgg tgtaggtata tggattatcc
     4141 acgtactgtt ctttcatcaa tgcagcagca tggggatatt ttatcatgtt caccaaccca
     4201 acatgaaaat catccggtaa atcctcacgt attttcatga tgggaaacct gctcgaatca
     4261 acaaccggtt ttggtccgat atgcgtttct gtataggttt tcattcccaa tgaaaccttc
     4321 ataattccct tctgggtatt aatctgggaa gcgtaaattt cgatgggttc caaacaaaac
     4381 caggtcttgt aggtttcgga aaccaacatg ggctggtaca atcctcgaat aacatcgcca
     4441 acgtaatctt tcaaatcgac atattcgcga atcgctttat caatttcctt ttcaataaat
     4501 tccttctgtc cttcaagtgc tgcatcagca atcatggtaa ccggcaattc aaaaagatac
     4561 agatcgattg caggtgattt gatccattcg tgtcctgctg atttggtgat cgtctcaact
     4621 ttccaattgt ctttaagtgt aattgccgac acgtaagttg ttttcaatat tccgttaaaa
     4681 cttgttgtta ccccgagggt tttgtactgg ccactaatac ttaaaggcaa tgttagtctg
     4741 atcttgtttt gtaatccttc tacttttata tcctcatgtt ttttcacttt cacctttagt
     4801 ccatcgttat cattatcggt aaaggaattg tcttcataaa tcacaccttt cacataggca
     4861 ttgatttgct tttgaatatc tgaaagggca atctccattg gaagattaat aaatgaaatc
     4921 tcccggttta tttctgtttt tacaggaaca tacgattcat ccggcttttc cggtttcaca
     4981 cttgttttgc aggaattgag cagcagtaaa acgctaaaac ccagaagagc agtaattcta
     5041 aactgagaaa acatagaatt tctttttagc taaaatagag aataataagt ccggtacaag
     5101 cccgctaatg
//
LOCUS       DAOMYG010000228         5107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5648_length_5107_cov_9.336302, whole genome shotgun sequence.
ACCESSION   DAOMYG010000228 DAOMYG010000000
VERSION     DAOMYG010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5648_length_5107_cov_9.336302"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="PK610_08250"
     CDS             complement(<1..834)
                     /locus_tag="PK610_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ35966.1"
                     /translation="MKRIFHLAVFLIAPFALSAQTDTSQVNPEAQQKYNEGVAAYESK
                     KLDEAISRFTEALALDPKFEKALLNRGNAYYEAKKTDKAIADYNTCGQLNGPSADNAW
                     YMKGTAEMDLGQYEEAVKSFTNAANKKNGEAKYHYNLGVAQFQAGNYDDAIGSFSNAI
                     RLKPDYAFAYNDRASAKKKKGDLDGAIKDYESAIQSNPQLTFAYNNLGSVKRQKGDFT
                     GAIQEYNNAIRLKKDYYIAYNNRGMAKMDAGKFEEAIADFNEAIKLKADYAFAYNNRA
                     AC"
     gene            926..1813
                     /locus_tag="PK610_08255"
     CDS             926..1813
                     /locus_tag="PK610_08255"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRJ35967.1"
                     /translation="MQAIVYFLALPFLYLLSILPFPVLYLVSDLLYLLLFKVLGYRKK
                     VVLTNLHNAFPGKSEQEYSILVKKYYRYLCDLFLETFKTLTISKKSMLKHCQLHPEAL
                     SLFQRYHAEGKSVIIVMGHYGNWEWAGNAFSLSCPQQLYVIYHPLRNKWFNGLICRMR
                     TRFGTKLYAMSDTFKEMVRNRKEINATAFIADQTPSPENAYWTEFLNQDTPVFWGTEK
                     IASKMGYAVVYVCIDRTKRGYYTVYAETLFENPSDTQDGQISESHSRRLESDIIKKPE
                     LWLWSHRRWKHKRPGIPAG"
     gene            1836..2810
                     /locus_tag="PK610_08260"
     CDS             1836..2810
                     /locus_tag="PK610_08260"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRJ35968.1"
                     /translation="MRTGKELILATKPFTKESKGLSWYYTISTLILLCGSIVTALLIE
                     ETIVRTLASLFAGLVMVRFFVIYHDHQHGAILSKSSVATIVFKLFGVLILAPSSIWKR
                     SHDYHHNHNSKLFSASIGSYPIMTREKFLKSTPSEKRSYLATRHPLTIFFGYFSMFLV
                     GMCVQSFSSSPKRHWDSLVALILHFSLQFIVFYFLGWEAWLFGIIIPFILADMIGAYL
                     FYAQHNFPGVTFREKEGWTYHHAALESSSYMKMNFFWNWVTANIGYHHIHHMNSRIPF
                     YRLPEVMKSIPELQNARETSLSIKDIVACFRLKVWDPERNKMISLKEI"
     gene            complement(2812..3969)
                     /locus_tag="PK610_08265"
     CDS             complement(2812..3969)
                     /locus_tag="PK610_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRJ35969.1"
                     /translation="MVRFITLVNLLFWAGQIQAQGIPFHKGDFASAQAIAKSEGKLIF
                     VDFYATWCAPCKFMANQIFPDTILQNYFTANFISLQIDAERQERELLNRVNVEAYPTL
                     VFFQADGKVIYHSKGAMDAPTLLETATMVREYEKNKKAYEKNPSNLRSLSLYLTVLSH
                     SDPIQARSIALKHLSSITTEKCTIPENWSLIRSFDRDPDSRFFLYSLENFRFFVEQMP
                     GYQEYFTGCAGLIIERAVELRDEKLVQRYKDVATAAIGKMNMPVSESFLYEIDAYYYS
                     QTGQRDKHLASLDQWMMDPITNAGVICNTVFETVDRYGVDAYPFTLKWAQRAVEMEKS
                     FFTLMTLATVYRSKGMKKEALQTAESAQKLAGPEDDINFYESFIQEVNQMK"
     gene            complement(3999..>5107)
                     /gene="purB"
                     /locus_tag="PK610_08270"
     CDS             complement(3999..>5107)
                     /gene="purB"
                     /locus_tag="PK610_08270"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRJ35970.1"
                     /translation="EIEKITNHDVKAVEYFLKEKFETLGVGEQKEFIHFGLTSQDINN
                     TSVPLSVKQVVSDVYLPLLDEVTDRLSTLAEEWKNIPMLARTHGQPASPTRLGKEILV
                     FAERLSKQRIQLTSIPYSAKFGGATGNFNAHHVAYPKIDWVAFSNKFVNKTLGLDRSQ
                     TTTQIEHYDNIASLFDAMKRINTILLDFDRDVWAYISMDYFKQKIKEGEVGSSAMPHK
                     VNPIDFENSEGNLGIANAILEHLSAKLPVSRLQRDLTDSTVLRNVGVPFGHTVIGLKS
                     LLKGLDKLLLNEATVRRDLEDNWAVVAEAIQTILRRESYPNPYEALKALTRTNKKIDA
                     NAISEFITGLEVSEAVKQELRSISPYTYTGITTL"
BASE COUNT         1451 a         1086 c          984 g         1586 t
ORIGIN      
        1 gcatgctgcg cgattgttgt aagcaaatgc ataatctgct ttcagcttta ttgcttcatt
       61 gaaatcagcg attgcctctt caaactttcc tgcatccatt tttgccattc cacgattatt
      121 ataagctata taataatcct ttttcaatcg aattgcatta ttatattcct gaattgcacc
      181 ggtaaaatca cctttctggc gtttaacgga accaaggttg ttgtaagcga aagtcaattg
      241 cgggttcgac tgaatcgcgc tttcataatc cttgatcgct ccgtcgaggt cgcccttttt
      301 cttctttgca gaagcgcggt cattgtatgc aaaggcataa tcaggcttta accgaatagc
      361 gttcgaaaat gaaccaattg catcatcgta attcccggcc tggaattgag ccacaccaag
      421 attatagtga tactttgctt caccgttctt tttattggca gcattcgtaa aacttttcac
      481 ggcttcttca tactgcccta aatccatttc tgcggtacct ttcatatacc aagcattatc
      541 tgcagatggt ccgtttagtt gtccgcatgt attataatca gcaattgctt tatccgtttt
      601 cttcgcctca tagtatgcat ttcccctgtt gagaagtgcc ttttcaaatt tgggatcgag
      661 agcaagcgct tctgtgaatc gggatatcgc ttcatcaagt tttttgcttt catacgcagc
      721 aactccctcg ttgtattttt gttgcgcctc aggatttacc tgactggtgt cggtttgggc
      781 tgacaaagca aatggcgcta tcaggaaaac agctaaatgg aatatcctct tcatgtttga
      841 gaaatttgaa tcttaaaaat agcaaaaatc cagccaatgc ctttgggaaa accatctaat
      901 cagctataat ctctattttt gagccatgca ggccattgtc tacttcctag cgttaccttt
      961 tctttatctg ctttccatcc tcccgtttcc ggtattatac ctggttagcg atcttcttta
     1021 cttgctatta ttcaaggttt taggttatag aaaaaaggtg gttttgacca atcttcacaa
     1081 tgcatttccc ggcaaatccg aacaagaata ttccattctg gttaaaaagt actaccgcta
     1141 tctctgtgat ttgtttctcg aaacttttaa aacattgaca atcagtaaaa aatcaatgtt
     1201 aaaacactgt caattgcatc cggaagcttt aagccttttt cagcgctatc atgctgaggg
     1261 aaagagtgta attattgtca tgggccatta tggaaattgg gagtgggccg gcaatgcatt
     1321 tagtctttcc tgtccgcaac agctctatgt gatctaccat ccattgagaa ataaatggtt
     1381 caatggactc atctgcagga tgcgtacccg tttcggaaca aaactatatg cgatgagcga
     1441 cacgttcaag gaaatggttc gcaaccgaaa ggaaatcaac gccaccgcat ttattgccga
     1501 ccaaacacct tcgcctgaaa acgcctattg gaccgaattc ctcaatcagg acacgcccgt
     1561 attttggggt acagaaaaga tcgcttcaaa aatgggatat gcagtggtgt atgtttgtat
     1621 tgaccgaaca aaaagaggat actacactgt ttatgcagaa acactttttg aaaatccatc
     1681 cgatacacaa gacgggcaaa tatctgagtc ccactcaagg aggttagaat cagacatcat
     1741 taaaaaacct gagctctggc tttggagtca cagaagatgg aagcataaac gtccgggaat
     1801 tccagcgggc tgagatttca ctatttttgc agcgcatgcg cacaggcaaa gaactaatat
     1861 tggcaacaaa accttttacg aaagaaagta agggcttgag ttggtattat accataagca
     1921 cattaatctt attgtgtggt tccatcgtaa ctgcattgct tattgaggaa accatcgtac
     1981 gtactttagc ttctcttttt gcaggattgg ttatggtgcg cttttttgtc atctatcacg
     2041 atcatcagca tggggcaata ctttcaaaat catctgttgc aactattgtt tttaaattat
     2101 ttggggtttt gattcttgca ccatccagta tttggaaaag gtcgcatgat taccaccaca
     2161 accataattc caaactgttt agcgcaagta ttggatctta cccaatcatg acaagagaaa
     2221 aatttctaaa atcaacgcct tcagaaaagc gctcctatct tgctacccga catcccctga
     2281 ctattttctt cggttatttt tcgatgtttc tggttggaat gtgtgtacaa tcgttttcaa
     2341 gttctccaaa gcgacattgg gattcattgg tcgcattgat tctccatttc tcattgcaat
     2401 tcattgtgtt ctatttcctt ggttgggaag cctggttatt cggaattatt attcctttta
     2461 tccttgcaga tatgatcggt gcttaccttt tttatgctca acataacttt cccggagtta
     2521 cttttcgaga gaaagaagga tggacctacc atcatgctgc cctcgagtct tcgagctata
     2581 tgaaaatgaa ttttttctgg aattgggtta ctgcaaatat cgggtatcat cacattcacc
     2641 acatgaattc aagaataccg ttttaccgtt tgcccgaagt gatgaagtcc attccggaac
     2701 ttcaaaacgc aagagaaaca tccttatcca taaaggacat tgttgcatgt tttcgtttaa
     2761 aagtctggga tcctgaacga aataaaatga tctcactaaa agagatttaa tttatttcat
     2821 ctggttgact tcctgaataa aggattcata aaaatttatg tcgtcttctg gtcccgccag
     2881 tttctgagct gattctgcag tctggagtgc ttcctttttc ataccctttg agcgataaac
     2941 agtagccaaa gtcatcagcg taaagaatga tttctccatt tcaactgctc tttgtgccca
     3001 cttgagggta aatggataag catcaacgcc atatcgatca acagtctcaa aaacggtatt
     3061 gcaaatcaca ccggcattgg taatgggatc catcatccat tgatcaagtg aagccagatg
     3121 cttatcgcgc tgaccggttt gggagtaata gtaggcgtca atttcgtata aaaaactttc
     3181 ggaaactggc atgttcattt taccaattgc agctgtagcc acatctttat aacgttgaac
     3241 aagtttttca tcccttaact caacagcacg ttcaataatc aaaccggcgc atcccgtaaa
     3301 gtattcctga tagcccggca tttgctcaac aaaaaatctg aaattttcga gtgaatacag
     3361 aaaaaatctg gaatcaggat cacgatcaaa acttctgatt aaagaccaat tctcaggtat
     3421 tgtacatttt tcagttgtaa tagatgacaa atgtttcaat gcaattgagc gagcctgaat
     3481 tggatctgaa tgcgataaaa ctgtaagata caaacttaaa gaacggaggt tggatggatt
     3541 cttttcatag gccttcttgt ttttctcata ttcccttacc atagtggcag tttccaaaag
     3601 cgttggagca tccatagcac cttttgaatg ataaataacc ttgccatctg cctgaaaaaa
     3661 aactagcgtt ggataagcct ctacattgac gcgatttaaa agttcccgct cctggcgttc
     3721 cgcatcaatt tgaagtgaga taaaattcgc agtgaaatag ttttgcaaaa tggtatccgg
     3781 aaatatctga ttcgccataa atttacatgg tgcacaccag gtggcataaa aatcaacaaa
     3841 aatcaatttc ccttctgact ttgcaatggc ctgagcgctt gcaaaatcac ccttatgaaa
     3901 agggatgccc tgtgcctgaa tttgaccggc ccaaaaaagc aggttaacta gtgtaataaa
     3961 tcgtaccata aacatttcct cacaaaattc agaccgtttt acaaagttgt tattcccgtg
     4021 tatgtatagg gagaaattga ccgaagctct tgctttaccg cttcactaac ttcaagtcct
     4081 gttataaact ccgaaatagc gtttgcatcg atctttttgt tggtacgggt tagcgcttta
     4141 agcgcttcgt aaggattggg ataggactct ctgcggagaa tagtctgaat tgcttccgct
     4201 acaacagccc aattatcttc gagatcacgg cgaacggttg cttcattcag caataactta
     4261 tcaagaccct tcagcaaaga tttcaatccg ataacggtat gtccgaacgg aacaccaaca
     4321 ttacgaagaa ctgtgctatc ggttaagtcg cgttgtaaac gtgaaacagg taattttgcg
     4381 gagagatgtt cgaggatggc atttgcaatt cccagattgc cctctgaatt ttcaaaatca
     4441 atcggattca ctttatgtgg cattgcggat gatcccactt caccttcttt gatcttttgc
     4501 ttaaagtaat ccatcgaaat atacgcccaa acgtcacgat cgaaatcgag aagaattgta
     4561 ttgattcgtt tcatcgcatc aaataaggac gcaatattgt cataatgctc tatctgggta
     4621 gttgtctgtg atcgatcgag tcccaatgtt ttatttacga acttattgga aaatgcaacc
     4681 caatcaattt ttggataagc aacatgatgc gcattgaaat ttccggtagc acctccaaat
     4741 tttgccgaat aaggaatact tgtcagctga atacgttgtt tcgaaaggcg ctctgcaaaa
     4801 acaagtattt cctttcccaa acgtgttggc gaagccggct gtccgtgtgt acgcgcaagc
     4861 attggtatgt tcttccattc ttcggccaat gttgaaagtc gatcggtcac ttcatcaaga
     4921 agaggcaggt acacatcact aacgacctgt tttactgaaa gcggaacaga tgtgttattg
     4981 atgtcttgag aggtaaggcc aaagtgaata aattcctttt gctctccaac acccagcgtt
     5041 tcgaattttt ctttgagaaa atactctacc gctttaacat cgtgattggt gattttttca
     5101 atttcct
//
LOCUS       DAOMYG010000229         5095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5661_length_5095_cov_8.937302, whole genome shotgun sequence.
ACCESSION   DAOMYG010000229 DAOMYG010000000
VERSION     DAOMYG010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5661_length_5095_cov_8.937302"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /gene="ccoN"
                     /locus_tag="PK610_08275"
     CDS             complement(<1..1333)
                     /gene="ccoN"
                     /locus_tag="PK610_08275"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HRJ35971.1"
                     /translation="MQTEKFHYDNKIVKMFAYATILWGLVGMLVGLIAALQMIFPWMN
                     FANEYTSFGRVRPLHTNAVIFAFVGNGIFMGVYYSLQRLCKARMFSDKLSKIHFWGWQ
                     LIIVLAAVTLLLGYTTGKEYAELEWPIDILITLIWVVFGWNMFGTIIKRRERHLYVAI
                     WFYIATFVTVAVLHLVNSFELPVSFFKSYSWYAGVQDALVQWWYGHNAVAFFLTTPYL
                     GLMYYFVPKAADRPVYSYRLSIIHFWALIFLYIWAGPHHLLYTATPDWVQSLGVVFSF
                     MLIAPSWGGMINGLLTLRGVWDKVREDVVLKFLVVAVTAYGMATFEGPLLSLKSVNWI
                     SHFSDWTIAHVHVGALGWNGFLTFGVLYWLIPKMFRTELYSKKLANWHFWIGTLGILF
                     YAIPLYWAGFAQAMMWKEFTDEGMLRYPNFLETVNQILPMYAMRAIGGTMFL"
     gene            complement(1342..1524)
                     /gene="ccoS"
                     /locus_tag="PK610_08280"
     CDS             complement(1342..1524)
                     /gene="ccoS"
                     /locus_tag="PK610_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HRJ35972.1"
                     /translation="MSALFIMIGASLLLALGFLIAFIWAVKKGQYEDDYSPSVRILFD
                     NPPSDSDNKSTTTEDK"
     gene            complement(1639..4065)
                     /locus_tag="PK610_08285"
     CDS             complement(1639..4065)
                     /locus_tag="PK610_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HRJ35973.1"
                     /translation="MNSTATQEKTSCYHCGEDCPSVPLIREEKKFCCEGCRTVYDILS
                     QNGMCEYYRYNETPGNNRKSPDRQRYDFLDDEEVASRFITYKDDNQTHVNFYIPGMHC
                     SSCIWLLENLNKLHPGILRSRVNFITKELSLQFSTNLKLPEIASLLDRVGYPPHLSMD
                     SLSEQKVKSSYFDKRYFYKLGIAFFSFGNIMLLSFPEYFGIDAITESPYRSLFGYLNF
                     VLALPVMFYSASEFFVSAWKGVRIRSLNMDFPIALGILVMFIRSSWEIFSGTGGGYMD
                     TLASLVFLMLVGRSLQNMTYGRLSFDRDYKSYFPVSVTRLFEGKEKGIPVNKVETGFR
                     LLIRNNELIPADAILFKGDAHIDYSFVTGESTPVEKTLGEIIFAGGKQIGNPIEVEVV
                     REVSQSYLTRLWNEDVFNTDKSEHERLNNLSNRISKWFTLVILLIATGAALYWFREDS
                     SRAMHAFASVLIITCPCALALSTPFTLGNTMRIMGKWNFYLRSTDVIEKLAKVDTILF
                     DKTGTLTQAGKAQLEYKGEPLDQNTLELISTATRNSTHPLSRSISEHLDEFHNEIKLD
                     SFQEIAGKGIIASYKSKEVKIGSASWILNGEPQKEDIRSTEVYISVNGTPKGKFIIMN
                     EFRPGLEHVINDLKQENEIVLVSGDNDSDLERLLPFFGNKAEMHFRYSPFQKLELVKE
                     KQAEQKNVLMIGDGLNDAGALRQSDVGISVSEDINNFSPACDAILDSDRFTMLPSLLK
                     FAKIAVVIILISFGLSFLYNIVGLWFAVQGNLSPLVAAILMPLSSISVILFTTGATAL
                     MAKRTLNNKG"
     gene            complement(4062..4481)
                     /locus_tag="PK610_08290"
     CDS             complement(4062..4481)
                     /locus_tag="PK610_08290"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HRJ35974.1"
                     /translation="MEYFEFYIPALAIAFARILLGVLLFYQGIDKVFGLGIKRVSETY
                     KQELNKAWIPSWVFPITAHYSSWVELIGGGLLILGLFKVVVLPLIAFDFLLVAMAMST
                     MSPLWDMRHLFPRVVLLLLVMIVPFMIDIFSLDHFLK"
     gene            complement(4481..4891)
                     /locus_tag="PK610_08295"
     CDS             complement(4481..4891)
                     /locus_tag="PK610_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35975.1"
                     /translation="MNLVFTEDTKICELNKQFHEHYPYLKLQFYEDKIDLHSHQEAPG
                     SPIKDENKTIHDILHGRVSGSISMNGHAKVKTFEHNWKEIAGLWVQVFRKSGNVWLQT
                     TVTDDLSLADVNRFGEEMAKDVEKDALPDIHEQE"
BASE COUNT         1536 a         1060 c         1020 g         1479 t
ORIGIN      
        1 tgaggaacat tgttcctccg attgcacgca ttgcgtacat gggcagaatc tgatttactg
       61 tttcaaggaa gttcgggtag cgaagcattc cttcgtcggt gaattctttc cacatcatcg
      121 cctgagcaaa tcctgcccaa tagagtggaa tcgcgtagaa caaaattccg agtgttccga
      181 tccagaaatg ccaattggct aattttttcg aatacagttc cgtgcggaac atttttggga
      241 tcagccaata gagaacacca aatgtcagga atccattcca tccaagcgct ccaacgtgaa
      301 cgtgcgcaat ggtccagtcg gagaagtgag aaatccagtt tactgattta agtgaaagaa
      361 gtgggccttc gaaggttgcc ataccgtatg cagtaacagc aacaacaagg aatttcaaca
      421 caacgtcttc acgaacttta tcccataccc cacgcaacgt gagtaatccg ttaatcatac
      481 caccccacga tggagcaatc agcatgaagg agaaaactac accaagtgat tgtacccaat
      541 caggagttgc tgtataaaga aggtggtgcg gtcctgccca gatgtagaga aagatcaaag
      601 cccagaagtg aataatcgaa agacggtaag aatatacagg acgatcagcc gcttttggaa
      661 cgaaataata cattaatccg aggtagggtg tagtaaggaa gaacgcaacg gcattgtgcc
      721 cataccacca ttgtaccaat gcatcctgca caccggcata ccatgaataa cttttgaaaa
      781 agctaaccgg taattcgaaa gagttaacaa ggtgaagaac ggcaacagta acgaatgttg
      841 caatataaaa ccaaatggca acgtaaaggt gacgctcacg acgcttgatg atggtaccaa
      901 acatgttcca accgaaaaca acccaaatca aggtgatcag aatatcgatc ggccattcaa
      961 gttctgcgta ttcctttcct gtagtataac caagcaagag agttaccgct gcaagtacaa
     1021 taattaattg ccagccccag aaatgaattt tgctcagttt atcgctgaac attcttgcct
     1081 tgcaaagtcg ttgtaaggaa tagtacacac ccatgaatat cccgtttccg acaaatgcga
     1141 aaataaccgc attcgtgtgt aagggacgaa cccttccgaa tgaggtatac tcattggcga
     1201 aattcatcca ggggaatatc atttgcagtg cggctattaa tccgaccagc atacctacca
     1261 atccccagag gatcgttgcg taggcaaaca tctttacgat tttgttgtcg taatgaaatt
     1321 tttcagtttg cagagttgtg ttcatttgtc ttcggttgta gttgacttat tatctgaatc
     1381 cgacggagga ttgtcgaata gtatgcgaac ggaaggggaa taatcatctt cgtactgacc
     1441 tttttttaca gcccatataa aggcaattaa aaaacccaat gcaagtagga ggctcgctcc
     1501 aatcattata aataatgcgc tcattgacga agtatcacgc gtcaaattta tctgtgccta
     1561 aaacgatgca tactgatgtg ggtcagtagt aaaactgaga tttatcagtt ttaagctaat
     1621 aagaagatta ttaactgatt accctttatt attgagggtt cgttttgcca tcaacgctgt
     1681 tgcgccggtt gtaaatagga tgacagaaat cgaacttaaa ggcataagaa tggctgcaac
     1741 caagggcgac aaatttccct gaacggcaaa ccaaagtcct acaatattat ataagaacga
     1801 cagaccaaaa cttatcagaa tgatcaccac agcaattttc gcaaatttta aaagtgaggg
     1861 tagcatcgta aatcgatcgc tgtccaatat tgcatcacaa gccggagaaa agttgttgat
     1921 gtcttctgaa accgatattc ctacatcact ttgtcgcaat gcacctgcat cattcaggcc
     1981 atcgccaatc attagtacgt ttttttgttc tgcttgtttt tcctttacca attccagttt
     2041 ctggaaaggg gagtagcgaa aatgcatttc agccttattt ccaaaaaaag gcaataagcg
     2101 ctcaagatcc gaatcgttat ctccggaaac caaaacgatc tcgttttcct gctttaaatc
     2161 attgatcaca tgttcaagtc ccggacggaa ttcattcatg ataatgaatt ttccttttgg
     2221 agttccatta accgaaatat aaacttctgt gctgcgaata tcttcttttt gaggttcgcc
     2281 attaagtatc catgatgctg aaccgatttt tacttctttg gatttatagg aagcgattat
     2341 tccttttccg gctatttctt gaaaggagtc gagtttaatt tcattatgaa attcatcaag
     2401 gtgttctgaa atacttcggc taagcggatg tgtagaattc cgggttgctg tactaattaa
     2461 ttccagggta ttttgatcta atggttcacc tttgtactca agctgcgctt ttcctgcttg
     2521 agttaatgtt cctgttttat caaagagaat ggtatcaact ttggctaatt tttcaataac
     2581 gtccgtactt cgcaggtaga aattccattt acccataata cgcattgtat ttcccaatgt
     2641 aaatggcgtt gacaatgcga gtgcgcaagg acatgtaata atgagtactg atgcaaatgc
     2701 atgcattgct cttgaagagt cttctctgaa ccaatacaag gcggctccgg ttgcaataag
     2761 aaggataacc aaggtaaacc atttactgat tcggttactc agattattta gtcgttcgtg
     2821 ttccgattta tcggtattga aaacatcttc gttccaaagt cgtgtaaggt aactttgtga
     2881 aacttccctt accacttcaa cttctatcgg gtttccaatt tgttttcctc ccgcaaaaat
     2941 gatttctcca agtgtttttt ccactggtgt ggattctccg gttacaaaac tgtaatcgat
     3001 atgcgcatcc cccttaaata gaatggcgtc ggcaggaata agttcattgt tgcgaatgag
     3061 taggcgaaat ccggtttcta ctttgtttac aggtattcct ttttcttttc cttcaaacaa
     3121 acgtgtaaca gatacaggga agtaggattt ataatcccga tcgaacgaca atctcccata
     3181 ggtcatgttt tgcaatgaac gacctaccag cataagaaat acaaggcttg cgagggtatc
     3241 catataaccg ccaccggtac ctgagaatat ttcccatgaa cttctgatga acatgaccaa
     3301 tattcctagt gcaatcggga aatccatgtt tagcgaacgg atgcgaacac ctttccatgc
     3361 gcttacaaaa aattcggaag ccgagtagaa cattacaggt aaggcaagga caaaattgag
     3421 gtaaccgaat aagcttctgt agggagattc ggtaatcgca tcaattccga aatactcggg
     3481 aaaactcaac aacatgatat ttccgaaact gaaaaatgca attcccagct tatagaaata
     3541 ccgtttatcg aaatagctgc tttttacttt ttgctcactc agcgaatcca tacttaaatg
     3601 aggagggtaa ccaaccctgt caagtaatga agcaatctct ggaagtttaa gatttgttga
     3661 gaattgtaat gaaagttctt ttgtaataaa attaactctt gatcgcagga tgccggggtg
     3721 aagtttgttc aggttttcca gtaaccagat acacgaactg caatgcatgc ctgggatgta
     3781 gaaattgaca tgagtctggt tgtcgtcttt ataagtgata aaccttgatg caacctcttc
     3841 gtcatccaga aaatcgtagc gctgacgatc aggcgatttt cgattgttgc ccggtgtttc
     3901 attgtaacgg tagtattcgc acattccgtt ctgactgaga atgtcgtata cggtacggca
     3961 accttcgcaa cagaattttt tttcctcgcg aatcaatgga acggacggac aatcttcgcc
     4021 gcagtgatag cagcttgttt tttcctgagt cgccgtagaa ttcatttcag aaaatgatca
     4081 agagaaaaaa tatcaatcat aaatggaaca atcattacaa ggagtaataa tacaaccctg
     4141 ggaaataaat gacgcatatc ccaaagtggc gacatcgtac tcattgccat tgcaacaagc
     4201 agaaagtcaa aagcaattag cggaagtaca acaaccttga ataatccaag gatcaaaagt
     4261 cctcccccaa ttaattctac ccaggaacta taatgtgcag taatgggaaa aacccaggat
     4321 ggaatccatg ctttattcag ttcctgtttg tatgtctcag atactcgctt aattcctagc
     4381 ccaaatactt tatctatccc ttgatagaaa agcagaacgc caagcaatat tctagcgaat
     4441 gcaattgcca gtgccggtat ataaaattcg aagtattcca ttattcctgt tcgtgaatgt
     4501 ccggtagagc atctttttca acgtcttttg ccatttcctc tccaaaacgg tttacatcag
     4561 caagcgaaag gtcgtcggtc accgttgttt gcaaccatac atttcctgat tttctgaaaa
     4621 cctgaaccca taatcctgct atctctttcc agttgtgttc aaaggtcttt actttggcat
     4681 gaccattcat gctgatagag ccgcttactc gtccatgcaa aatatcatga atggttttat
     4741 tttcatcttt tataggtgaa cccggagctt cctgatgaga atgcaaatca attttgtctt
     4801 cgtaaaactg aagcttcaag taagggtaat gctcgtggaa ttgtttattc agttcgcaga
     4861 ttttagtgtc ttctgtaaat acaagattca ttgttccggt ttttatgatt gaatggttgt
     4921 gagtgcttgg taaaatagaa ctgatttttc agaactcatc tttgttgatt ttccattcct
     4981 tttcaggtag aaccaacaag ggaatgtgtg tgtgatatgc catttttttg ctgaatgatt
     5041 taccaaacag ccggtcgaac attccatgtt tccgatgaac aaaggccaac cagtc
//
LOCUS       DAOMYG010000230         5092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5668_length_5092_cov_8.619218, whole genome shotgun sequence.
ACCESSION   DAOMYG010000230 DAOMYG010000000
VERSION     DAOMYG010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5668_length_5092_cov_8.619218"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..657
                     /locus_tag="PK610_08300"
                     /pseudo
     CDS             49..657
                     /locus_tag="PK610_08300"
                     /inference="COORDINATES: protein motif:HMM:NF019583.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
     gene            637..1461
                     /locus_tag="PK610_08305"
     CDS             637..1461
                     /locus_tag="PK610_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 associated domain-containing protein"
                     /protein_id="HRJ35976.1"
                     /translation="MQGGTIVLEFGEEKTILENLPKYLATNSIPKAKQNKYVPIPYFS
                     TGEETYRDSLVIQIISPDPFCSIYYTTDGIAPNVKAKKYNSPLVVKESIVLKAIAVDP
                     TGVVSKLNFGSFNKIDHNWSVVIQSSVNPQYTAGGPFALVDGLLGDQNWRKGRWIGVQ
                     GQDFESVIDLKHSTRIMNVRANFLEDTRAWIWLPDEVSFFYSEDGKEWKEWRTIQNDS
                     ISNENSERIKGFEIKKSGMIQARYLKVKAKNHGKIPAWHPGSGNDAFIFIDEVQIN"
     gene            complement(1631..2941)
                     /locus_tag="PK610_08310"
     CDS             complement(1631..2941)
                     /locus_tag="PK610_08310"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRJ35977.1"
                     /translation="MKIAVVGTGYVGLVTGTCFAETGNDVICVDIDKEKVEKMRNGIV
                     PIYEPHLDVLFERNIKAGRLKFTTDLAEAIHPAQIVFLALPTPPGEDGSADLSYVLGV
                     AEQLGKMIKDYKVIVDKSTVPVGTAEKVKGAVAKNAMVDFDIVSNPEFLREGFAVDDF
                     LKPDRVVVGTSTARARTLMEELYKPFVRSGNPIIFMDEKSAELTKYAANAFLATKITF
                     MNEIANFCERVGADVDMVRIGIGSDSRIGKRFLFPGIGYGGSCFPKDVSALAKSGKEA
                     NFEFKIINAVMEVNERQKLRLIDKVRAYYKNDLSGKTFALWGLAFKPDTDDIREAPAL
                     YMITDLLAAGAKVRAFDPEAMENVKKIFGDKIEFTNDEYESIQGADALLIATEWSEFR
                     NPDFDRIGKSLKEKVIFDGRNLYDLSQMRKMGFYYESIGRETVQ"
     gene            complement(2977..4107)
                     /locus_tag="PK610_08315"
     CDS             complement(2977..4107)
                     /locus_tag="PK610_08315"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRJ35978.1"
                     /translation="MKKIQMVDLLTQYEKVQGEIDTAVLNVLRSSAYINGPEVKEFQK
                     ELESYLGVKHVIPCANGTDALQIAMMALGLQPGDEVITASFTYVATAEVIALLRLTPV
                     LVDVDPNTFQIDPEKVKKAITPKTKAIVPVHLFGQCSDMEAIMKIAKEHNLFVVEDNA
                     QAIGAEYIYSDGNRKKAGTIGTIGCTSFFPSKNLGCFGDGGAIFTNDDDLAGKLRMIA
                     NHGQSVQYVHDSIGVNSRLDSIQAAILRIKLRSLDNYCKARNKAADYYDKAFSSNSKI
                     TTPARAKNSTHVFHQYTLLLADGVDRNALREFLAAKGIPAMIYYPIALHMQKAYTDPR
                     YKEGDFPVTENLCKRVISLPMHTELDEEQLKFITDAVNEFVR"
     gene            4216..4965
                     /locus_tag="PK610_08320"
     CDS             4216..4965
                     /locus_tag="PK610_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35979.1"
                     /translation="MRFLLLLYTLILSSLCFSQASIKDSSILVTEIKMSGAAQVPVGQ
                     LAQRFSTNASIGLEVYVKTPSQLGFGLQGTFIFGNNVKETSILDGLRDSNGQIIDLNG
                     EYARVLLQERGFTITGQFGYLIPVLSPNPNSGILIKFGAGFMSHKIRIGTEKNPVPQL
                     TGEYLKGYDRLTYGLTLQQFVGYQYLNNRRMINFFFGFEIFEGFTKNRRAFNFDQVQA
                     DHSMRTDILTGIRAGWILPLYPRPAKAMYFN"
BASE COUNT         1422 a         1051 c         1057 g         1562 t
ORIGIN      
        1 tgaccggcgt cggctcggtg ctctccttcc tcccccagat catcattctg ttcctcgtta
       61 tcgccgttcg atcctcggga agtgaataat cattttactg aagcaaattc atggcaatac
      121 agtttttttg ttccgcatga tatcgatggg atgattcagt tttttggagg agcgaaacat
      181 tttgaagaaa agttgaactc gcttttcacg gcatcttctg caacgcttgg tcgtgatcag
      241 gctgatatta ctggtttggt tggtcagtat gcacatggaa atgagccaag ccatcatatg
      301 gcatatctct ataattacgt aggtcgtccc gataagaccc agaaaattat aaatcagata
      361 atgagttcat tttacacagc ggctcctgat ggtttgattg gcaacgagga ttgtggtcaa
      421 atgagtgcct ggtatgtttt atctgcgatg gggttctatc aggttacacc aggtgatcca
      481 ttttatagtg taggagttcc tttgtttgat caagtaaaaa taaaagtgga tgatgaaaaa
      541 atttgcacga tccattcaca atcccgttct gataagaatg cttttgttac aagagtaaat
      601 ggtgatttca gaatgtcggt cagtcatcag gaaataatgc agggtggtac aatagtgttg
      661 gaatttggtg aggaaaaaac tattctggaa aatctgccta aatatttagc aacaaattct
      721 attcccaagg ctaaacagaa taagtatgtt ccgattccct atttttcaac aggcgaagaa
      781 acctatcggg atagtttggt gattcaaata atttcaccgg atcctttctg ctcgatttat
      841 tatacaacgg atggaattgc acccaatgtg aaagcaaaaa aatacaattc tccattggtg
      901 gtaaaagagt caatcgtcct taaggcaatt gctgttgatc caaccggtgt tgttagtaag
      961 cttaattttg gatcctttaa caagattgat cataattggt ctgttgttat ccaatcatcc
     1021 gtcaatcctc aatatacagc gggaggtcca tttgctttgg tcgacggatt attaggtgac
     1081 cagaattggc gcaaaggccg atggataggc gtgcaggggc aggactttga atccgtaatt
     1141 gacttaaagc actccactcg gataatgaat gtcagagcaa attttcttga agatacccgc
     1201 gcatggatat ggcttccgga tgaagtttct tttttctatt ctgaagatgg taaagaatgg
     1261 aaagaatggc gtacaattca gaatgattcc atctctaatg aaaattcgga acggataaag
     1321 gggtttgaga tcaaaaaatc aggaatgatc caagccagat atttaaaagt aaaagcaaag
     1381 aaccacggca aaatcccggc ctggcatcca ggatcaggta acgatgcatt catctttatc
     1441 gatgaagtgc agatcaattg aatcttccac ctgaaaccat cctaaaacaa attaattccc
     1501 ttagacctcc gggattttta aaatcccgga ggtctgggca aagttattgg gtttgaaatt
     1561 tagaatcaag ccggaaaata ccaaatgtgc ttgttcgttt acggttcggc cgttgatgga
     1621 acggttagat ttattgcaca gtttcgcgac cgatactttc gtagtagaat cccattttgc
     1681 gcatctggct aagatcgtaa agatttcgac cgtcaaagat tactttttct ttgagggatt
     1741 ttccaattcg atcgaagtca ggattgcgga actcagacca ttctgttgca atgagcaatg
     1801 catcggctcc ttgaatggat tcgtattcat cgttcgtgaa ttctatttta tcaccaaata
     1861 ttttcttcac attctccatc gcttcagggt caaaagcacg cactttggct ccggcagcaa
     1921 gaagatcggt gatcatgtac aatgccggtg cttcgcggat gtcatcggta tcgggtttaa
     1981 atgccagacc ccacagcgca aacgtttttc cacttagatc atttttgtaa taggcacgaa
     2041 ccttatcgat caaacgaagt ttctgacgtt cgtttacttc cattacagcg ttaataatct
     2101 tgaattcgaa attggcttct ttacctgact tcgcaagggc agagacatct tttggaaagc
     2161 agctgcctcc atagccgatt ccgggaaaaa gaaagcgctt accaattcgt gaatctgagc
     2221 caattccaat tcgtaccata tctacatctg ctccaacacg ctcacaaaaa ttagcaatct
     2281 cattcatgaa agtgattttt gtagcaagaa atgcatttgc agcatacttt gtaagttcag
     2341 ccgatttttc atccatgaaa atgatggggt ttcctgaccg cacaaagggt ttgtacaact
     2401 cttccattaa tgttcttgca cgtgcagtag aggtaccaac aacaacacgg tctggtttta
     2461 aaaagtcatc cactgcaaat ccttcacgca aaaattccgg attggaaaca atatcgaaat
     2521 caaccatcgc atttttagca acagctccct tcactttttc tgctgtgcca acgggtactg
     2581 tacttttgtc aacgatcact ttgtaatctt tgatcatttt tccaagttgt tccgcaaccc
     2641 caagcacata cgaaagatcc gcagaaccat cttctccggg aggagtaggt aatgcaagaa
     2701 aaacaatctg tgcaggatgt atggcttcag ctaaatctgt tgtgaatttt aatcgtcctg
     2761 cttttatatt tctttcgaat aaaacatcaa ggtggggttc gtaaatggga acaatcccat
     2821 tgcgcatctt ctcaactttt tctttatcga tgtcgacgca aataacatcg ttgcctgttt
     2881 ctgcaaaaca agttccggta accaatccga catatcctgt tccaacaact gcaattttca
     2941 taataggtag attaaaaatt ctttttactg ctattcttat cgcacaaatt catttacagc
     3001 atctgtaatg aattttaact gctcttcatc aagttccgta tgcatgggta atgaaattac
     3061 acgcttgcat aaattctctg taacaggaaa atcgccttcc ttataacggg gatcagtgta
     3121 tgctttctgc atgtgaagtg caatagggta gtagatcatt gccgggattc cttttgcagc
     3181 aagaaattct cttaacgcat tgcggtcaac accatcagca agcagcagtg tgtactggtg
     3241 gaaaacatgt gtcgaatttt ttgcacgtgc gggcgtggta atttttgaat tggaagaaaa
     3301 tgctttgtcg taataatctg cagctttgtt tctcgctttg caataattat cgagtgagcg
     3361 aagcttaata cgaagtattg cagcctgaat gctgtcaagg cgagaattca caccgatcga
     3421 atcgtgaacg tactgtacgc tttggccatg atttgcaatc atgcggagtt ttcctgccag
     3481 atcatcgtca ttggtgaaaa ttgcaccacc atctccgaag cacccaagat tttttgatgg
     3541 gaaaaaggag gtacatccta tagtgccaat agttcctgct tttttacgat taccatcgct
     3601 gtaaatatat tctgcaccaa ttgcctgtgc attatcttct acaacaaata aattatgctc
     3661 tttagcaatt ttcattatgg cttccatatc ggaacattgc ccgaaaagat gaaccggaac
     3721 aatagctttt gttttcggtg ttattgcctt ttttactttt tcaggatcaa tctgaaaggt
     3781 attcggatca acatccacca ataccggggt gaggcgtaac aatgcgatca cttctgcagt
     3841 ggctacataa gtaaacgagg cggtaatcac ttcatctccc ggttgcagac caagtgccat
     3901 catcgctatc tggagtgcat cggttccgtt tgcacaggga ataacgtgct tcacgccaag
     3961 ataggattcg agctccttct gaaattcctt gacctctggt ccgttgatat aggccgaaga
     4021 acgcaataca ttaagtaccg ctgtatcgat ttctccctga actttctcat actgtgtgag
     4081 aaggtcaacc atttgtatct ttttcatgtt cgaaaagtat aggttgcgaa tatactcaaa
     4141 aacagaaccg ttaaaaggta aaatctactg tagaaaggga tactatgcat tcggtttttg
     4201 cgttactttt gatctatgcg attcctcctt ctcttataca ccctgatttt gtcttcttta
     4261 tgcttttccc aggccagcat caaggattct tctattttag ttaccgaaat caagatgagt
     4321 ggagctgcac aagtccctgt aggtcaactc gcgcagcgct ttagtaccaa tgcgtccatt
     4381 ggactcgaag tgtatgtgaa aacgccttct caactggggt ttggtcttca ggggacattt
     4441 atttttggga ataatgttaa ggaaacctcc attctcgacg gattaaggga ttccaatgga
     4501 caaatcatcg atcttaatgg cgagtacgca agggttctgc ttcaggaaag gggatttacc
     4561 attacaggtc aattcggata tctgattcct gtgttgagtc caaatcctaa ttccggaata
     4621 ttgataaagt tcggagccgg attcatgtcg cacaaaattc gtattggcac agaaaaaaat
     4681 cccgttccac agctcacggg tgaatacctg aaggggtacg accgattaac ctatgggctt
     4741 acgctgcagc agtttgttgg ctatcagtac ctgaataaca gaaggatgat caattttttc
     4801 tttggttttg aaatatttga aggctttacc aaaaacagga gagcgtttaa tttcgatcag
     4861 gttcaggccg atcattcaat gcggacagac atcctcactg ggattcgcgc cggatggata
     4921 ttgccactgt accctcgtcc tgcaaaggca atgtatttca actgatccgt tttttcagtt
     4981 cagaatcaag ctcgtttttt ggattatctt tggttgatga ttctgcttta tcgtttatca
     5041 attctgcttt atgttgcagc tattcgcatt gcttccttgt ttaatgccaa gg
//
LOCUS       DAOMYG010000231         5083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5678_length_5083_cov_9.904137, whole genome shotgun sequence.
ACCESSION   DAOMYG010000231 DAOMYG010000000
VERSION     DAOMYG010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5678_length_5083_cov_9.904137"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PK610_08325"
     CDS             complement(<1..725)
                     /locus_tag="PK610_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35980.1"
                     /translation="MAIALVPLKKSGIESCDLLIMVKNRQQLNHHKELSGHITLLTEK
                     LKAEKSELITFYQGSRKIVVGMFFNKDKDRSYKLENIRAFGNRIGKQFNALKSSEIAI
                     VGLKEVFSKEENFALLEGLELGNYQFLKYKSKKTDAYTLKSIQLEAGTLTETEIREFS
                     TLMDAVTLTKDLVNEPVITLDAVKFSKEIEKAGKANGFSVEVFNKSKIQSLNMGGLLG
                     VNMGSEVPPTFNILTYKPRGAKNT"
     gene            complement(819..1502)
                     /gene="elbB"
                     /locus_tag="PK610_08330"
     CDS             complement(819..1502)
                     /gene="elbB"
                     /locus_tag="PK610_08330"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HRJ35981.1"
                     /translation="MKKFAVLLSGCGVYDGSEIQEAVFTLLSISENGASYQCFAPNID
                     QHHVINHLTGEEMNEKRNALTEAARIARGEIKDLSAYEAGDFDGLVIPGGFGAAKNLT
                     KWAFEGPAGDINAEVKRSILDTINTNKPMAGLCMGPTVIAKALQGTSTKAELSVGTTT
                     EASPYDIGAISQGMEAAGAKAVMKSIREIAIDKQHNIVSAPCYMMEGSIVDVRKNIDQ
                     AVKKLIELA"
     assembly_gap    1537..1636
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1962..3020)
                     /locus_tag="PK610_08335"
     CDS             complement(1962..3020)
                     /locus_tag="PK610_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018942397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HRJ35982.1"
                     /translation="MKKLTLLSFSALVLFSSCFVEENKSDVKTDNKDTLPQEVNLYSH
                     RHYDIDREIYAEFEKETGIKVNVIEDDADKLMVRLKSEGENSPADILITADAGRLLKA
                     KEEGLLQSVKNETLESAIPANLRDEEGFWFGLTMRARVIVTHKDRVKEGAISSYAELA
                     DKKWKKKVLIRSSENVYNQSLIAALIANWGYDKTLAWAKGIVANMARDPKGGDKDQVL
                     AIAAGEGDIAVVNTYYLGKMLSSENPEEVKAAEMVRVIFPDQNGSGTHINISGAGIVK
                     TSPNKANAIRLLEYLTSAKVQEKFAKGNNEYPVVIGVATNELLQGWGDFVKDSLPLSN
                     LGKYNTDALKLFNEAGWK"
     gene            complement(3069..3983)
                     /locus_tag="PK610_08340"
     CDS             complement(3069..3983)
                     /locus_tag="PK610_08340"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HRJ35983.1"
                     /translation="MHSPQTVFLVRPDHFGFNAETAQSNAFQKNDQSANAELAKEEFN
                     TVIELLRREGIDHLVFPSREDVTTPDAVFPNNWISILPDGTLILYPMMAVNRRLERNM
                     AIVQELHQKFPVQTIIDLSEAEQSGHFLEGTGSIVFDHTHKIAYACISPRTDEKLLNE
                     LSKRIGYRAFAFEAIGPKSEQIYHTNVVMSVAENYAIVCLDAVPDTIQAQMLKSSLEL
                     TGKSVIPISFAQMNQFAGNMLEVRDKNGKAVLLMSSTAFHSLDTGQITEIEKHVRIAH
                     IAIPTIETLGGGSLRCMLAGIQIPRKGE"
     gene            4222..4971
                     /locus_tag="PK610_08345"
     CDS             4222..4971
                     /locus_tag="PK610_08345"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRJ35984.1"
                     /translation="MKIFMPIFVLLLLSSCSETQSEENEEKQLVVDTLSETVPVPVPI
                     VDTCELERRLIAAGLVAIEMVDPTILVDLKYSRDDNFMKEDVYGDLTRLYLQPDVAKK
                     LAKAQQLLKKHDSTLTLLVYDGVRPRSIQQMLWDKVDLPITEKGKFVSNPKNGSLHNY
                     GAAVDITIARISGEAIDMGANYDDTAMAAYPANEARFLKEGILTQEHIDNRKLLRKVM
                     QGAGFFGIQTEWWHFNSCTRAQAKEWYTIIE"
BASE COUNT         1272 a         1089 c         1078 g         1544 t
ORIGIN      
        1 gtgttttttg ctccacgggg tttgtaagtg agtatattga aagtaggagg gacttcactt
       61 cccatattca cacccaatag acctcccata ttgagcgact gaattttgct tttattgaaa
      121 acttcaacac tgaatccgtt agcttttcca gccttctcaa tttcctttga gaatttaaca
      181 gcatccaaag taatcaccgg ttcatttacc aaatctttgg ttaacgtaac agcatccatc
      241 aaagtactga actcacggat ttcagtttcg gttaatgtgc ctgcttcgag ttgaatgctt
      301 ttcagtgtat atgcatccgt ttttttgctc ttgtatttta gaaattgata attccccaat
      361 tcaagtcctt caagcaacgc gaaattttct tccttggaaa aaacttcttt gagtccgaca
      421 atcgctattt ccgaagattt taatgcgttg aattgttttc cgatacggtt tccgaaggca
      481 cggatgttct cgagtttgta ggagcggtct ttatctttat taaaaaacat ccccacaaca
      541 atttttctgc taccctggta aaaggttatc agttcacttt tttctgcttt cagtttttct
      601 gtaagcaaag tgatgtggcc ggatagttct ttgtgatgat ttaattgctg acggttttta
      661 accatgatca gcagatcaca ggattcaatt ccggattttt tgagcggcac taatgcgata
      721 gccataagaa agatgtttat gtaaaagtaa gagtcaatgc cgagaatggc aaaagttaaa
      781 caccaggaaa ggaaaagagt ttcttaatag ttgattattc aggctaattc aatcagtttt
      841 ttcaccgcct gatcaatatt ttttctgaca tccacaatcg aaccttccat catgtagcaa
      901 ggcgcagaaa cgatattgtg ttgcttgtcg atggcaattt cacgaatgct tttcatgaca
      961 gccttggctc ccgctgcttc cattccctga ctgatagcac cgatatcata gggagatgct
     1021 tcggttgttg ttcctaccga taattcggct ttggttgatg ttccctgcaa ggctttggcg
     1081 ataacggttg gtcccatgca caagcctgcc attggtttat tggtgtttat ggtatcgaga
     1141 atacttcgct ttacttcggc gttaatatca ccggccggtc cttcgaaagc ccatttggtg
     1201 aggtttttgg cagctccaaa tccaccgggg ataacgagtc cgtcgaaatc tcccgcctcg
     1261 taggcgctaa ggtccttgat ctcacctctt gcgattcttg ctgcttctgt tagggcattg
     1321 cgcttttcat tcatctcctc accggtaagg tggttaatga catgatgctg atcaatattg
     1381 ggtgcgaaac actggtagct cgctccgttt tcagaaatgg ataacagcgt aaaaacagct
     1441 tcctgaattt cggatccatc ataaactccg caaccggaga gaagaacagc gaactttttc
     1501 atgatctaaa tatagcaaaa ggtgaacgga aataatnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnngtgt gaatgtgtga atgtgtgaat gtgtgaatgt gtgaatgtgt
     1681 gaatgtgtga atgtgtgaaa aactggtaat ttacgaaaag caaggtgtat ctattgaaat
     1741 ttgcttattt aagataccta agaagaacaa gtcggaaccg gactaacagg atttcagcat
     1801 tctcaaatat gtcctacaaa gcttcagcca aaaatggggc gcgcattcct ctcattcgca
     1861 cattcccctc attcgcacat tcctctcatt cgcacgttcc tctcattcct ctcattcgct
     1921 cattcctctt attcgctcat tcccctcatt cgcacattca tttacttcca acccgcctcg
     1981 ttaaacagct tcagcgcatc tgtattgtat tttccaaggt tggaaagggg tagagagtcc
     2041 ttcacaaaat cgccccaacc ttgaaggagt tcattggtag caacaccaat aactacagga
     2101 tattcgttgt ttccttttgc aaatttttcc tgcactttag cactagtcag gtactcgaga
     2161 agacggatgg cgttggcttt gttaggtgaa gtttttacaa taccggcgcc actgatgttg
     2221 atgtgtgttc cgctaccgtt ttgatcaggg aaaatgacgc gtaccatttc tgccgctttc
     2281 acttcttccg gattttcgga ggagagcatt tttccgagat aataggtatt cacaactgca
     2341 atatctcctt ccccggctgc tatagccagt acttgatctt tatcgccacc ctttggatca
     2401 cgcgccatat tggcaacaat gcctttggcc catgcaagtg ttttatcata tccccaattg
     2461 gcaattaatg cagcaatcag cgattggtta tacacgtttt cagatgagcg aatcaatact
     2521 tttttcttcc attttttatc ggccaattct gcatagcttg atatagcgcc ttccttcaca
     2581 cggtctttgt gtgttacgat cacacgtgca cgcatggtta gaccaaacca aaatccttct
     2641 tcatcacgca gattagctgg aatagcagat tcgagtgttt cgttttttac ggattgcaac
     2701 aaaccttctt ctttggcttt cagtaagcgt cctgcatctg cagtaataag aatatctgcg
     2761 ggtgaatttt ctccttcgct ttttaaacgg accatcagtt tatcggcatc gtcttcaatc
     2821 acattcacct taattcctgt ttccttttca aattcagcgt agatctcgcg gtcgatatca
     2881 taatgtcggt gcgaatacag attgacctcc tgtggaagag tgtccttgtt gtcggttttt
     2941 acgtcagatt tgttttcttc aacaaaacaa gagctgaaaa gtacaagtgc ggaaaatgaa
     3001 aggagggtta actttttcat ggtttaaggt taagtcggct gcaaatatag agggcaaccg
     3061 aatacctttt actccccttt tcgaggtatt tgaattcccg ccagcatgca ccgcaaacta
     3121 ccgcctccca gggtttctat ggttggtatt gcgatatggg caatccgcac atgcttctca
     3181 atttcagtga tttgtccggt atcgagggaa tggaaagcag tggagctcat caacaggaca
     3241 gcttttccgt ttttatcccg gacctccagc atattgccgg caaattggtt catctgtgca
     3301 aacgaaatgg gtatgacaga cttgccggtc aattcgagcg aggatttcag catttgcgct
     3361 tgtatggtat caggaacagc atcgaggcaa acgatggcat agttttccgc caccgacatc
     3421 accacgttgg tgtggtagat ctgttcgctt tttggtccga tggcttcgaa tgcaaatgcg
     3481 cggtagccga tccgtttaga cagttcgttc agcagctttt catcggtgcg cggcgatata
     3541 caggcgtagg caattttgtg ggtgtggtca aagacgatac ttcctgttcc ttcgagaaaa
     3601 tgtccggatt gctcggcttc actgagatcg ataatggttt gtaccggaaa tttttgatgc
     3661 aattcctgaa caatagccat gttgcgttcc aacctccgat tcacagccat catcggatac
     3721 aaaatgagcg tgccatccgg aagaatactg atccagttat tggggaaaac cgcatcaggt
     3781 gtagttacat cttctctcga aggaaaaacc aaatgatcaa ttccttccct acgcagcagt
     3841 tcaatcaccg tgttgaattc ttccttcgcc agttctgcat tggccgactg atcgttcttt
     3901 tgaaaggcat tgctttgtgc agtctctgcg ttaaacccaa aatgatcagg tcggacaaga
     3961 aaaacggttt gcggtgaatg catttgcgaa tttacttgtt tttgatttgt aatcggttat
     4021 cagttaagag ttattggtta tcagttaaaa ggtattaggt atttgtgtaa ttgggtaatt
     4081 ggtaattgtg tattagttga gggttatcag gtaatccaga agcttaaggt cgggacaggt
     4141 tggatgttgg attcgtttta cgttctccgt tctcgttctc cgttcactgt ttaccaaaac
     4201 gaaaaatggc taatttgcgc tatgaaaatt ttcatgccca tttttgtttt attgctactg
     4261 tcttcctgtt ccgaaaccca atcggaagag aatgaagaaa agcaacttgt tgtggatacc
     4321 ttatcagaaa ccgttcccgt tcctgttccc atagtagata cctgcgagct tgaacgtcgc
     4381 ctgattgctg cgggactggt ggcgattgag atggtggatc cgacgatctt ggtcgatttg
     4441 aagtattcga gggacgacaa tttcatgaag gaagatgttt acggagatct tacccgtttg
     4501 tacctgcaac cggatgtcgc caaaaaactg gcgaaggcac aacaactcct caaaaaacac
     4561 gattccacgc ttacactttt ggtttatgat ggtgttcgtc cgcgaagcat acagcaaatg
     4621 ctatgggaca aggtggatct gcccatcaca gaaaaaggga agtttgtttc caatcccaaa
     4681 aacggatcac tgcacaatta cggtgcagcg gtggatatca ccattgcccg aataagtgga
     4741 gaagcgattg atatgggcgc caattacgac gacaccgcca tggctgccta tcctgccaat
     4801 gaggctcgtt ttcttaaaga ggggattctg acgcaggaac acatcgataa ccggaaacta
     4861 ttgcgaaaag tcatgcaggg agcaggtttc tttgggatcc agaccgaatg gtggcacttc
     4921 aattcatgta cccgtgccca ggctaaggaa tggtacacca tcatagagtg accgaacctt
     4981 tttggattat ctttgttaat tagaaaaaca aagcgactat gcgttccatt ttcattatta
     5041 cctcattggt tctttctttg tctgctgtgt atgcttttca gca
//
LOCUS       DAOMYG010000232         5082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5681_length_5082_cov_8.288442, whole genome shotgun sequence.
ACCESSION   DAOMYG010000232 DAOMYG010000000
VERSION     DAOMYG010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5681_length_5082_cov_8.288442"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /gene="trmB"
                     /locus_tag="PK610_08350"
     CDS             <1..811
                     /gene="trmB"
                     /locus_tag="PK610_08350"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRJ35985.1"
                     /translation="RVLITFFAIMGKNKLQRWAEMEGFPNVIQQSGKELLEADHPIKG
                     HWKKGMFHNDHPITLELGCGKGEYTVGLAKRFAERNFIGVDVKGARIWRGAKTAVDES
                     IKNAAFLRTRIDFIKAFFSKDEVDEIWLTFSDPQPREGKENKRLSSPYFLENYCSFLK
                     PDGLIHVKTDSDLLYVFTREVINKYKLKCLVDTADLYGDQFESFDTSTRDILDIRTHY
                     EQMWLAKGKKIKYLKFELPANFKMNPELRIELDPSLAELEKYYKTIEYKTR"
     gene            792..1154
                     /locus_tag="PK610_08355"
     CDS             792..1154
                     /locus_tag="PK610_08355"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRJ35986.1"
                     /translation="MSTKPVKLSEKNKDFFDLVYQVVRLVPYGRVTSYGAIAKYLGSA
                     RSSRMVGWAMNASHVQKGVPAHRVVNRNGELTGKMHFSSPDEMQQLLEAEGIVIVDDT
                     VIDFDKLFWDPVKELGFE"
     gene            complement(1206..4070)
                     /locus_tag="PK610_08360"
     CDS             complement(1206..4070)
                     /locus_tag="PK610_08360"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRJ35987.1"
                     /translation="MKRTLRITAGIASLCMLAGATSAQIAFTNGSSRLTNSVKSTNVL
                     TVADWNNDGLDDIIRLHEGYQLVIDEQRPGQNFLEHNFGTVGSGSGWAWGMAVGDFDG
                     NGYKDVVAGGYSSGTPLRVIMMNSTGTGITIQNLTPGGFFVQNITLGDINNDGHLDIF
                     VCDDNAMSHIFINDGNGAFTHNTTLMNFDVTATDDSGNYGSVWTDYDNDGDLDLYIAK
                     CRQSVSNPADGRRINVLFRNNGNGTFTEAAAPANINVGWQSWSASFGDIDNDNDFDLV
                     LTNHDYAAQLFINNGSGVFTENTTSGINTTGFTPIQSGFEDFDNDGFVDLLITGSSGA
                     KMFRNNGDLTFSQITVPFQAAPAIYTPSWGDLNHDGFIDVFASYGTIYNNPSSTNNDR
                     LYLGTPNGNHFLCVNLTGTISNPDAVGARVTIYGPFGLQTREVRAGESYGNQYSGLIH
                     FGLGSNTQVDSILIDWPTSGVQVIENPTVDNFITVVESNCVSPLTGNITSSNGSFAFC
                     TGQSVTLQAPTGTGYTYLWSNGSTASSINVNTTGEYSVVITQSGNACPAISPSVNVTE
                     NPDETPTISANGPTSFCYGESVTLAGPTGMNGYDWSNGEQTSSIVVTQAGTYSLDVTG
                     ACATFSSNNIVVDVMTAPSPTTSDVSIPVPGTANLSATGNSVVWYDAAVGGNQVGVGN
                     NWTTPFLNTTTSFFAEDMEIFGGDQYSGGKIYGPTPAAYGGNTTNPKTFFNVTEAGTL
                     ESVKVYTDRYGIRRIQVFNNAAQLVAFVDADIQADTVVVPLNIDLTPGTGYYITTDGA
                     TNSNIPGNAGNTTPRLMRHASGITFPYNVGPAISITGNDLSVGYYYFFYDWRFKTTEF
                     DCPGPRAEAIVTVVSPVGLNEVTEGGFRIFPNPTRDFLNIESKGNYAIEIIDLTGRAV
                     YSMSNANGNQRIDVNSLIPGVYFVRLNGASGKTIQKIVIQ"
     gene            complement(4150..>5082)
                     /locus_tag="PK610_08365"
     CDS             complement(4150..>5082)
                     /locus_tag="PK610_08365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ35988.1"
                     /translation="LDLDIIGTPYAAQDSLYIDLSLLNKAGHKFPSGYPSRRAFVQMI
                     VIKSNGDTLFRSGLMQPNGEIMHNTGPYQNHYSMINQETQTQIYEMIMGDVMGDRTTV
                     LERAALHLKDNRIPPLGFTTTHSAYDTIQIVGNASSDTNFNRNGATEGTGKDILHYHI
                     PLNGFMGEVSIYTKVYYQTVNPGWLNEMFAYNTPAIDSFRNWYNQSDQTPLLVARDSL
                     LQLALNVSVQDNLSNHIQVYPNPISDGIIRIENQGIVIQSLSIYDLTGRLIGTYSGQE
                     IKALNNLQLPSSSGTYILKINTEEGQLTKKVIRY"
BASE COUNT         1451 a         1000 c         1156 g         1475 t
ORIGIN      
        1 aagggtctta ataactttct ttgcaattat gggcaagaac aagctgcagc gctgggctga
       61 aatggaaggt tttccgaatg tgatacagca gtcgggaaaa gagctgttgg aggcggatca
      121 tccaatcaaa ggccattgga agaaaggaat gtttcacaat gatcatccca ttaccctcga
      181 attaggttgc ggtaagggtg agtatactgt aggattggca aagcgttttg cggaacggaa
      241 ttttattgga gtagatgtaa aaggcgcccg gatttggaga ggagcaaaaa ctgctgtgga
      301 tgaatcgatc aaaaacgcag cttttcttcg gactcgaatt gattttataa aagcgttttt
      361 tagtaaagat gaagtggatg agatctggtt gactttttct gatccacaac cacgtgaagg
      421 caaagaaaac aaacgactca gctccccgta ttttctcgag aactattgtt cttttttaaa
      481 gccggatgga ttgattcatg taaaaacaga tagtgatttg ttgtatgttt ttacacgtga
      541 agtgatcaac aaatacaaat tgaaatgttt agtagacacg gctgatcttt atggcgatca
      601 atttgaatcg tttgatacat ctacacgcga tattcttgat atccggacgc attacgaaca
      661 aatgtggctt gcaaaaggaa aaaagatcaa gtatctgaaa tttgaattgc ctgctaattt
      721 taagatgaat cctgaattgc gaattgaact cgatccttct ttggccgagc ttgaaaaata
      781 ctataaaacc attgagtaca aaacccgtta agttaagcga aaagaacaaa gattttttcg
      841 atctggttta ccaggtagta cgccttgttc cttatggtcg tgtaaccagt tatggagcaa
      901 tagccaaata ccttgggtct gcgcgttcat cacgtatggt gggatgggca atgaatgctt
      961 cacatgtgca aaagggcgtg cccgctcacc gggtagttaa tcgcaatgga gaattaaccg
     1021 gaaagatgca cttttcttct cctgatgaaa tgcaacaatt gctcgaagca gaaggtattg
     1081 ttatcgttga tgatacagta attgattttg ataaactttt ctgggatcct gtaaaggagc
     1141 taggatttga atagcaaaaa aaaatcccga ctgtaaagcc gggatttttt cattgtgcaa
     1201 atttattact gaataacaat cttctgaatg gttttaccgg atgcaccatt taggcgaaca
     1261 aagtaaacac cggggataag cgaatttaca tcaatgcgct ggttgccatt agcattgctc
     1321 atagagtaaa cagcacgtcc tgtaagatcg ataatttcaa tcgcatagtt tcctttgctt
     1381 tcgatgttaa ggaagtcgcg tgttgggtta gggaagatgc ggaatccgcc ttctgtcact
     1441 tcatttaatc caacgggact tacaaccgta acaatagctt cagcacgagg gccgggacaa
     1501 tcgaattctg ttgttttaaa tctccagtcg tagaagaaat aatagtaacc gactgataaa
     1561 tcatttccgg taatgcttat cgccggccca acattataag ggaatgtgat cccggatgcg
     1621 tggcgcatta aacgtggagt tgtattaccg gcgttccctg gaatgtttga atttgtagct
     1681 ccgtcggtag taatgtaata tcctgtacca ggagtaaggt cgatgttcaa aggaacaact
     1741 acggtgtcgg cctgaatatc ggcatcaaca aaagcaacca attgcgctgc attgttgaat
     1801 acctgaatgc gacgaatacc gtaacgatca gtgtaaactt tcacgctttc cagtgttcct
     1861 gcttcagtaa cgttaaagaa tgtttttgga ttggtagtgt taccaccgta agctgctggt
     1921 gttggtccgt agatcttacc gccactgtac tgatcgccac cgaaaatttc catgtcctca
     1981 gcaaagaaag atgttgttgt attgaggaaa ggagtcgtcc agttgttacc aacgccaacc
     2041 tgatttccac caactgctgc gtcgtaccat acaacactgt taccggttgc agatagattt
     2101 gcagttccag gaaccggaat tgaaacgtct gaagttgtag gtgatggagc agtcattaca
     2161 tccaccacaa tgttgttgga tgaaaaagtt gcacaagctc ctgttacatc aagtgagtag
     2221 gttcctgcct gggtaaccac tattgatgaa gtctgctctc cgttggacca atcgtaacca
     2281 ttcatcccgg ttgggccggc aagcgtaacg gattcaccgt aacagaaaga agttggtccg
     2341 ttcgcagaaa tagtaggtgt ttcatcaggg ttctcagtca cattaactga aggagaaata
     2401 gcaggacaag catttcctga ttgggtaata accacactgt attcacctgt agtatttacg
     2461 ttgatggaag aagctgttga tccgttgctc cagagataag tataaccagt tcctgttgga
     2521 gcctgaagtg ttacactctg tcctgtacag aatgcgaatg aaccattgga agaagtgatg
     2581 ttacctgtaa gaggagaaac acaattgctt tcaacaacag taatgaaatt gtctacggta
     2641 ggattttcaa tcacctgaac tccgcttgtt ggccaatcaa taaggataga atcaacttgt
     2701 gtgttgctgc ctaatccaaa atgaatgagt ccggagtact ggtttccata agattcaccg
     2761 gcgcgtactt cgcgggtttg aagtccgaat ggtccgtaaa tcgtaacacg tgcaccaact
     2821 gcatcaggat tggaaattgt accggtgaga tttacacaga ggaagtgatt tccatttggt
     2881 gtgccaagat aaaggcggtc gttgttggta gacgatgggt tgttataaat ggttccgtaa
     2941 gaagcaaaca catcgatgaa accatcatgg ttcaaatctc cccatgacgg agtataaata
     3001 gcaggtgctg cctgaaatgg aacggtaatt tgggaaaagg taaggtcacc attgttacgg
     3061 aacatttttg ctccggaact acctgtgatt aataagtcaa caaaaccatc attatcgaaa
     3121 tcttcaaatc cactctgaat tggtgtgaat cctgttgtat tgattccgga tgttgtgttt
     3181 tctgtgaata caccgcttcc gttgttgatg aataactgag cagcgtaatc gtggttggta
     3241 aggaccaagt caaaatcatt atcgttgtcg atatcaccaa aacttgcgct ccatgactgc
     3301 catccaacgt tgatgtttgc aggtgcagca gcttctgtga aggtgccgtt accgttattg
     3361 cggaaaagta cgttgatacg acgaccatca gcaggattac ttaccgactg acgacatttc
     3421 gcaatataaa gatcgaggtc accatcatta tcgtaatctg tccaaacaga accgtagtta
     3481 ccggagtcgt ctgttgcagt tacatcgaaa ttcatcaatg ttgtgttgtg tgtaaatgca
     3541 ccgtttccat cattgataaa aatgtgcgac atcgcattat cgtcacaaac aaaaatatcg
     3601 aggtgaccat cattgttaat gtctccaagc gtaatgtttt gtacaaagaa tccaccgggt
     3661 gtcaggtttt gaatggtaat tccagtaccg gttgaattca tcatgataac acgaagaggt
     3721 gttccggagc tgtatcctcc agcaacaaca tctttgtaac cgttaccatc aaaatcacct
     3781 acagccattc cccaagccca acctgaaccg cttcctaccg ttccgaagtt gtgttcaaga
     3841 aaattctgac cagggcgctg ctcgtcaatt accaactgat aaccttcatg gagtcggatg
     3901 atatcatcca aaccatcgtt gttccagtcg gcaacggtga gtacgttagt actttttaca
     3961 ctgttcgtaa gccttgagct tccgttcgtg aaggcaatct gcgcagatgt ggctccagcc
     4021 aacatacaaa gcgacgcgat acctgcggtg attcgtaaag ttcttttcat tttcagttag
     4081 taaatgtgca cgctgaaata tagccaatat tggttaagtt tttaagcgat ttggacgaaa
     4141 aggttaatat tagtaacgaa tgaccttttt cgttagctga ccctcttctg tgttgatttt
     4201 caggatgtaa gtaccgctgg aacttggtaa ctgcaagtta ttcagtgctt tgatttcctg
     4261 tcctgaataa gtccctatca atctgcctgt caggtcataa atgctcagtg attgaatgac
     4321 aataccttgg ttttcaatgc gtatgattcc gtcagaaatg gggtttgggt agacttgtat
     4381 atggttgctc aggttatcct gcacagaaac gtttaatgca agttgaagca gtgaatcacg
     4441 ggctaccaac aaaggcgttt ggtcggattg gttgtaccaa tttctgaagg aatcaattgc
     4501 aggtgtgttg taggcaaaca tttcattcaa ccatcctggg ttaactgttt ggtaatagac
     4561 ctttgtgtaa atactcacct ctcccataaa accgttgagt ggaatatggt aatggagaat
     4621 atcctttccg gttccttctg ttgcgccatt gcggttaaaa ttggtgtcgc ttgatgcgtt
     4681 gcctacaatt tgaatggtgt cgtaggctga atgagttgtt gtgaaaccta aagggggaat
     4741 acgattgtct tttagatgca aagcagctct ttcaagcaca gttgttctgt cgcccattac
     4801 atcacccata atcatttcat aaatctgggt ttgtgtttcc tgattgatca tggaataatg
     4861 attctggtaa ggacctgtgt tatgcatgat ctctccattg ggttgcatca aaccggatcg
     4921 aaacaaggta tcgccatttg attttatcac aatcatttgt acaaacgctc ttctggaagg
     4981 atatccggat ggaaatttat gtccagcttt attcaataga gataaatcga tatacaacga
     5041 atcctgagca gcgtatgggg taccaatgat gtccagatcc ag
//
LOCUS       DAOMYG010000233         5076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5687_length_5076_cov_8.190599, whole genome shotgun sequence.
ACCESSION   DAOMYG010000233 DAOMYG010000000
VERSION     DAOMYG010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5687_length_5076_cov_8.190599"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..307)
                     /locus_tag="PK610_08370"
     CDS             complement(20..307)
                     /locus_tag="PK610_08370"
                     /inference="COORDINATES: protein motif:HMM:NF013790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35989.1"
                     /translation="MTREQAKSRIDQLTAELNRHNDLYYIKSQPEISDFEFDKLLEEL
                     IHLETNFPEFVHSDSPSQRVGGDITKDFPVVKHTYPMLSLSNSYSKEEIIE"
     gene            complement(399..1316)
                     /locus_tag="PK610_08375"
     CDS             complement(399..1316)
                     /locus_tag="PK610_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35990.1"
                     /translation="MRLVVCIFLTVHSLCSFGQNMNTDIWTLDIELKPDSVYFSNPQN
                     ITSGHKGYDNQPWFSPDGKYIFYTAMTEHSTTDIFRYEIEGRKSIQLTTTPRTSEYSP
                     RITTDGTGISVVRVEEDGTTQRLWRFDLNGKKPKKIAPWLDSVGYYQFLSGELIVAFI
                     LGPAEQHGLRLINLKTKEEQVICNHVGRWFSGHLLKIPTRETSYENHTIGVYYTLSTA
                     SKSLLLDFWMNGPDMARKLIQLPEGAQDLLALTTGWLASSGDEIWFLSNQPNSDWKPL
                     PRKGFPSLKGISRMAIHPNGKTIALVCSE"
     gene            complement(1382..4705)
                     /gene="secA"
                     /locus_tag="PK610_08380"
     CDS             complement(1382..4705)
                     /gene="secA"
                     /locus_tag="PK610_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRJ35991.1"
                     /translation="MLGLLKKIFGSKKEKDIAEIKPYIDKINQEYAQLSGLSHDAFRA
                     RTQDLRKKIADFVHPEEAKIEEIRNKANVTTDMDEKERLLEEADKMVKLVDEKLEIIL
                     LEILPQAFAIVKETGRRFKENPEIAVTATPFDRELSVSNKVVSIDGDKAIWKNTWDAA
                     GTTITWDMVHYDVQLLGGVVLHQGKISEMMTGEGKTLVATLPIFLNALTGKGVHVVTV
                     NDYLAKRDSEWNAPIFMFHGLAVDCIDKHEPNSDRRRKAYRADIVYGTNNEFGFDYLR
                     DNMANSVDHLVQQKLHFAIVDEVDSVLIDDARTPLIISGPTPQGDKHEFYELKPKIER
                     LVAEQRKYINLCLTDAKKLLSGLGEDGRGNVDKDSLEKGGMAVLRAHRGLPRNKALIK
                     FMSEPGVKAIMQKTESQYMAEQQKEMHKVDKELFFVIDERNNQIELTEKGIELLSSGE
                     DSSYYIMPDVGAEIAKIQASGLATQEQTQLRDTLMRDYTIKAERIHTMNQLLRAYAMF
                     ERDVEYVIMDGKVKIVDEQTGRIMEGRRYSDGLHQAIEAKENVKVEAATQTYATITLQ
                     NYFRMYHKLAGMTGTAETEAKELWDIYKLDVVTIPTHRAIVRKDENDLVYKTAREKYN
                     AVIDEIASLREAGRPVLVGTTSVEISELLSRMLKLRNIPHQVLNAKLHQKEADIVAFA
                     GQSGTVTIATNMAGRGTDIKLGPGVKEAGGLAIIGTERHESRRVDRQLRGRSGRQGDP
                     GSSQFFVSLEDNLMRLFGSERIARMMDRLGMKEGEVIQHGMITKSIERAQKKVEENNF
                     GIRKRLLEYDDVMNVQRKTIYSKRRNALYGDRMDIDLSNMFYDVAESLVAEYQDVRDY
                     DTFRFECLRNLAIDAPVSEDEFLSGDTSEIAGKLFDLAMKTYRRNMDGMLERIYGVVK
                     NVYETQSSQYENIVVPFTDGIRTLQSVVNLKESYESGGRAALRTAEKSVLLAVLDNEW
                     KEHLREMDDLKQSVQQAVYEQKDPLVVYKLESYELFKKLIDKMNKEVISFLVKAQLPV
                     NTGEQQVQKAPAPRQELPKNVQTSRTEIPSYGDPSSSGDQQQRVKTQPVRVEKKVGRN
                     DACPCGSGKKYKNCHGIGE"
BASE COUNT         1372 a         1180 c         1020 g         1504 t
ORIGIN      
        1 tgctttcgct ttccaattcc tattcgataa tctcttcttt cgaataggaa ttggaaagcg
       61 aaagcattgg ataggtatgt ttcacaacag gaaaatcctt tgtgatatct ccaccaacac
      121 gctggctcgg ggaatcgctg tggacgaatt ccggaaaatt tgtttcgagg tgaatcagtt
      181 cctccagtaa tttatcgaat tcaaaatcgc tgatttcggg ctgacttttg atatagtaca
      241 aatcattatg cctgttcaac tcggccgtaa gctgatcaat tctggatttt gcctgttccc
      301 ttgtcataat gtaaagttaa tgagagcagg aatttgaaag cggtaaaaaa ggagatttta
      361 tcaacggaat cgcatcggta cgtgaataaa atcggggttt attcggagca aaccaaagca
      421 attgtttttc cattgggatg aatggccatt cgtgagattc ctttcaggga agggaatcct
      481 ttccgaggca atggtttcca atcggaattc ggctgattgg ataaaaacca gatctcgtcg
      541 cccgagcttg caagccagcc tgttgttaat gccaataaat cctgagcacc ttccggcagt
      601 tgaataagtt tccgggccat atcaggacca ttcatccaaa aatcgagtag caaactctta
      661 gatgctgtgc taagcgtata atacacccct attgtatgat tctcgtagga cgtttccctt
      721 gtaggtatct taagcagatg tccgctgaac caacggccaa catgattgca aatgacctgt
      781 tcttcttttg ttttcagatt aattaatcgc aaaccatgct gctcagcggg acccaaaata
      841 aaagctacga tcaattcacc ggaaagaaat tgataatacc caactgaatc cagccagggt
      901 gcaatttttt ttggcttttt tccattcaag tcgaagcgcc ataaacgctg ggtggtaccg
      961 tcttcctcca cacgaaccac cgatattccg gttccatcgg tagtgatgcg cggactgtat
     1021 tctgaagttc ggggtgtagt agttaattga atggatttac ggccttcaat ttcatagcga
     1081 aaaatatccg tggttgaatg ttcggtcatt gccgtgtaaa aaatatattt tccatccggc
     1141 gaaaaccaag gctgattatc ataacccttg tgacctgaag taatattttg cggattactg
     1201 aaataaactg aatccggttt caactctata tcgagggtcc agatatctgt attcatgttt
     1261 tgtccgaaag aacaaagcga atgaacagtc agaaaaatgc acactactaa tcgcatactg
     1321 tttcaaaatc atattattat ttaatccgaa aaaaaagagc ccccaaatgg aggctctttt
     1381 tttattcacc aattccgtga caattcttgt atttttttcc tgagccacaa ggacaggcat
     1441 cgttacgtcc aactttcttt tccactcgca ccggctgagt ttttacccgc tgttgttgat
     1501 caccacttga agaaggatca ccgtaacttg gaatttcagt tctggatgtt tgaacatttt
     1561 ttggtaattc ctgacgagga gcaggagctt tttgtacctg ttgttctccc gtattaacag
     1621 gaagctgtgc cttcacgagg aaagagataa cctctttgtt cattttatcg atcagctttt
     1681 tgaataactc gtacgactca agtttataaa ccaccaacgg atctttttgc tcgtaaactg
     1741 cttgctgaac actttgcttg aggtcatcca tttcgcgcaa atgttccttc cattcgttgt
     1801 ccaataccgc cagcaatact gatttttccg ctgtacgcaa ggcagcacgt ccaccgcttt
     1861 cgtacgattc tttcaggtta accaccgact gcaaggtgcg gataccatcg gtgaaaggaa
     1921 ccacgatatt ctcgtactga ctactttgag tttcatacac atttttaacc acgccgtaga
     1981 tgcgctcaag cataccatcc atatttctac ggtaggtttt cattgcgagg tcaaaaagtt
     2041 ttcctgcaat ttcacttgta tcacctgaaa ggaattcatc ttcagaaacc ggagcatcaa
     2101 ttgcaagatt gcgtaaacat tcaaaacgga aagtatcgta atcgcgcaca tcctgatatt
     2161 cggcaaccaa tgattcggca acatcgtaga acatattgga aagatcgatg tccatacgat
     2221 cgccatacaa ggcattgcga cgtttagaat aaatggtctt tctttgaaca ttcataacgt
     2281 catcgtattc gagcaatcgt ttacgaatac cgaagttatt ctcctccact tttttctgcg
     2341 cacgctcaat ggatttggtg atcataccat gctgaatcac ttcaccctct ttcattccaa
     2401 gacggtccat catacgcgca atacgttcag atccgaacaa acgcatcaga ttatcttcaa
     2461 gagaaacgaa gaactgtgaa gaaccgggat caccctgacg accggaacga ccacgcaact
     2521 gacgatcgac acgtcgcgat tcgtggcgct cagtaccaat gatcgctaaa ccaccggctt
     2581 ccttcactcc gggtccgagt ttaatatcag taccacgacc tgccatgttg gtagcaatgg
     2641 taactgtacc cgattgtccg gcaaatgcaa caatatccgc ttctttttgg tgaagctttg
     2701 cgttaagcac ctggtgagga atattgcgta gtttgagcat tcggctgagc aactcagaaa
     2761 tttctactga agttgttcct acaagaacag ggcgtcctgc ctcccgtaat gatgcgattt
     2821 cgtcaataac cgcattgtat ttttcacgtg ctgttttgta aacaaggtca ttttcatcct
     2881 tacgtacaat tgcgcgatgt gttggaatgg ttacaacatc cagtttgtag atatcccaca
     2941 attcttttgc ttctgtttca gcagtaccgg tcataccagc aagcttgtgg tacattctga
     3001 aataattctg caaggtgatg gtcgcatagg tttgcgttgc agcttccacc ttcacattct
     3061 ctttcgcttc gatagcctgg tgcaatccgt cggaataacg gcgtccttcc atgatacgac
     3121 cggtttgttc atcgacgatt tttactttac catccatgat cacatattcc acatcacgtt
     3181 caaacatcgc ataagcacgc agcaactgat tcatggtgtg gatacgctca gccttgatag
     3241 tataatcacg catcaaggta tcgcgcaatt gggtttgttc ctgagttgca agtccggaag
     3301 cctgaatttt tgctatttct gcgccgacat ccggcatgat gtaatagctg gaatcttcac
     3361 cggaagacaa taactcaatc cctttttccg taagctcaat ctgattattg cgttcgtcaa
     3421 taacaaagaa caattctttg tcgaccttat gcatttcctt ttgctgttca gccatgtatt
     3481 ggctttcggt tttttgcata atggctttta cgccgggttc tgacatgaat ttgatcagcg
     3541 ctttattacg aggcaaacca cggtgagcac gcaatacagc cattccccct ttttcaagtg
     3601 aatccttatc gacatttccg cgaccatctt caccaaggcc ggataataat tttttcgcgt
     3661 cggtcaggca caggttaatg tattttcttt gttcagcaac caaacgttca atttttggtt
     3721 tgagctcata gaattcatgt ttatctcctt gcggagtagg accggaaatg atcaacggag
     3781 ttcgcgcatc atcaatcaat acagaatcga cctcatccac aattgcaaaa tgcagtttct
     3841 gctgtacaag atgatcaact gaattggcca tgttatcacg caggtaatcg aaaccaaatt
     3901 cgttattggt accataaacg atatctgcac ggtaagcttt tcgtctgcgg tcggagttgg
     3961 gttcatgttt atcgatgcaa tcaacggcca gaccatggaa catgaaaatc ggagcgttcc
     4021 attcggagtc acgcttagcc aggtaatcgt ttactgtaac aacatgtaca cctttaccgg
     4081 taagtgcatt caggaaaata ggaagtgtag ccacaagggt ttttccctca ccggtcatca
     4141 tctccgaaat tttcccctgg tgcaatacaa caccacccag caactgaaca tcataatgca
     4201 ccatatccca ggtaatggtt gttccggccg catcccaggt atttttccag atcgctttat
     4261 cgccatcaat acttacaact ttgttgctta cactcagttc acgatcgaaa ggtgttgcag
     4321 ttacggcaat ctccggattc tccttgaagc ggcgaccggt ctcttttaca atagcaaatg
     4381 cctgaggcaa aatttcgagc aggataattt caagtttttc atccaccagt ttcaccattt
     4441 tgtcggcctc ttcgagaaga cgctccttct catccatatc ggtggtgaca ttggctttgt
     4501 tacggatctc ctcaatttta gcttcttcgg gatgaacaaa atctgctatc tttttacgca
     4561 gatcctgagt acgggcacga aatgcatcat gactgagtcc gctgagctgt gcatattcct
     4621 gattgatctt atcaatgtat ggtttgattt cagcaatatc cttttccttt ttgctaccga
     4681 aaattttctt gagtaatccg agcatgatgc gttttgattg gtaagtggta agtcgacaaa
     4741 agtaaacaat atttacgcat ccggcccctt cagaacacac atgggcagac ctgagcctca
     4801 ggtgtttgaa gcagaaagct tcgtaagcac cccaacacac ccaaaaaccg gacctgaggt
     4861 aattcttcag acagatcagc agaaaggctg acagaaaggc gttttcctgc ttttttttag
     4921 ggctgaagcc tgtagattat gccatttagg atgcaaggaa aaaaccgtcc ttcaaccaat
     4981 gcgcattttt agcacctttg cagagtgatt gtttatccca acgcaaaaat aaatctcgga
     5041 ctcaatatcc ttcgcaaaag aaacgatgga taccat
//
LOCUS       DAOMYG010000234         5061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5717_length_5061_cov_9.684978, whole genome shotgun sequence.
ACCESSION   DAOMYG010000234 DAOMYG010000000
VERSION     DAOMYG010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5717_length_5061_cov_9.684978"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="PK610_08385"
     CDS             complement(<1..708)
                     /locus_tag="PK610_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35992.1"
                     /translation="MLFVAAFCYTEQHSLRAQTIDKSGVITVRKLKDNNPRIAGKTGG
                     DITPAELCNALGITSQIAGVKIIHYEITYYRGGEVTREVEGNQVPPEICSYLNTTKPG
                     MMITFEKILAQDASGIKFQLNPMRFKLVEGGSPAKENVSITREINLNGFYQLRSNSSN
                     IEKTYLKLYNDSVVFIITSRDDAVIVNQGLEREYKKSGTAHGKYKINQGRLFIDLDNS
                     ISRFDYEGTYTASGLMLN"
     gene            complement(793..2640)
                     /locus_tag="PK610_08390"
     CDS             complement(793..2640)
                     /locus_tag="PK610_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35993.1"
                     /translation="MKPKIIIDREPVSSEEIQSRKDFRKVVSQVALMQKPFFKSTWFI
                     SSAVITGTMAAIAVVAIFAAHPPTEDQQEEALISEVKSDSIPANALLGSGYYSEDSPC
                     IKPPVPGAELQASTYTVDNQKGAVLKHSTGTEIRIPANSLTDESGKVIQGKAELQYRE
                     YHTVPEILLSGIPMTYDSAGTRYAFETAGMFDIHCVQNGKKVYLDEKKPISVKMQSTS
                     AEGRFNFYELDTAARNWVYLGKGKNEAIVNENEDVKEVITDVKEVNTNEEITTHHSVQ
                     SHPEVIKKTGMVEQAKKEVAKTEESKPVEPRKLNPDKYNFLVNVDPEEFPEMASFKST
                     RFEVGEESKNFDRTWFSKTWNDVRLREKSKGISYYLDLTVIQNGKPNTSTVVVYPAFD
                     GKDYEMARKRFDQQFTTYEKTLSDKKADLAKKEAELKAEIKKQEIEFARQEMARKEAE
                     KERERIRKQQIADANRISTNSYGQMVLISTGNQVRMMAARTFSVAKFGTFNCDSPHKF
                     PSERVLSATFSGKTEGEEHPIVAMETFLVNRKTKMYYPYSSSMLNQFGYNPKGDNMIL
                     IILADGKTAVFSAEDFQKIPNEVPMYHFTMRISDAPVRDASELEKLIFG"
     gene            complement(2640..3221)
                     /locus_tag="PK610_08395"
     CDS             complement(2640..3221)
                     /locus_tag="PK610_08395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ35994.1"
                     /translation="MLETGSRYHKTERQLQDELQVVEAAKRDPARFEPLYNAYYEQIF
                     RYIHQRIDDKDTAFDLCSQVFLKALTNIGKYQFRGVPFASWLYRIAQNEVYEALRSHQ
                     TRRVVSIETRQVSEFLEEIEEGHSEEMFRLLEKVLPELPEEDLQMIEWRFFEKRSFRE
                     IGDILGITENNAKVKGHRSLEKLKKMFKTYIPK"
     gene            3428..>5061
                     /locus_tag="PK610_08400"
     CDS             3428..>5061
                     /locus_tag="PK610_08400"
                     /inference="COORDINATES: protein motif:HMM:NF016994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HRJ35995.1"
                     /translation="MIRTLFLLLTIVAFATNARAQDTTKLDIADNGDYYSFRMLDNVI
                     NNYRIIFTGENHVFRKSNYRLELKMMKYLYHKAGFRHFIMEFGFSRGWLVDKYVQTGD
                     TSLFKVLSDYSYQEYALLYKGLYEFNKTLPDTAKIRIHSIDIERSLSTPLKVMDLLIP
                     KNRKIPASIALTIESIHSLAVMSDDEQRKAQQKNKEDFRTGAPFSLKRSLKFILQDLD
                     SNKAAFKELLGNDYPVFYKIEQGLRAEMDRSTYNDMSAVQGYLYRERFMHESLRELAA
                     EFPTGKFYGQFGRCHTSLETEDVWCGFYAFTTLATRLDSDSLFKGKVLAIAQYYPNGS
                     AHEKGVIDRDGVKPLLKISSPDGLTLIHHRDVVNDTARLKNRFQYLIINQADHRMDNM
                     KVDSTKPPVDYSTYESTIFLEGHYGLLGVNLNALNSTLFGLDPSAISFTSPLTVVGGS
                     YTQLEDGVFYTFMYSAIRNTEIGFSDSLYYGLKGGSFRFRVGGDFVRSKWLDVGPFFG
                     YGYQRLVLTRDEILAGPVGSGFFGATNRLRTSVTNPS"
BASE COUNT         1259 a         1072 c         1128 g         1602 t
ORIGIN      
        1 attgagcatt aatccgctgg ccgtataggt gccttcataa tcgaaacgtg aaatcgaatt
       61 atcgagatcg ataaacaatc ttccctggtt gattttgtat ttcccgtggg cggtgccgga
      121 ttttttgtac tcgcgttcca aaccctgatt tacaattaca gcatcgtctc gcgaggtgat
      181 gataaaaacg acactatcgt tgtagagttt caaataggtt ttttcgatgt tggaggaatt
      241 gcttcgtaat tgataaaatc cgttgaggtt aatttcccgt gttatcgata cattttcttt
      301 tgccgggctc ccaccttcca ccagtttaaa gcgcatcggg ttcagttgaa attttattcc
      361 tgatgcgtcc tgcgcgagta ttttttcaaa agtgatcatc ataccgggct tggtggtgtt
      421 caggtaggaa cagatctccg ggggaacctg attgccttct acttctctgg tgacttctcc
      481 tccgcggtaa taggtgatct cgtagtgaat gatttttact ccggcaatct gagaggtaat
      541 tcccaaggca ttgcacaatt ctgcaggggt gatgtcgcct cctgtttttc ctgcaatcct
      601 ggggttattg tcttttagtt tacgcaccgt aatcactccg gatttatcaa tggtttgtgc
      661 gcgtaacgaa tgttgttccg tgtaacagaa tgcggctaca aacagcatga agtagattga
      721 taatttccta aagcaatggt gtgttggcat agactcaaaa cgcatttggt ggcagaaggt
      781 tgcaggagat catcagccaa aaatcaattt ttcaagttca cttgcatccc tcaccggtgc
      841 atcgctgatg cgcatagtaa aatggtacat gggcacttca ttcggaatct tttgaaaatc
      901 ctccgctgaa aacacagctg ttttaccatc tgcaagaata atcagaatca tattatcccc
      961 tttgggattg tagccgaatt gattaagcat cgacgatgaa tagggataat acattttcgt
     1021 tttccggttt accagaaatg tttccatggc aacaatggga tgttcttctc cctctgtttt
     1081 tcctgaaaat gttgcggaaa gaacacgctc agatgggaat ttgtgggggg agtcgcaatt
     1141 gaaggttccg aattttgcaa cagagaaagt acgtgctgcc atcatgcgaa cctgatttcc
     1201 ggtgctgatc agaaccattt gtccgtagct gttggtgctg atgcggttgg cgtctgctat
     1261 ttgttgtttg cgtatgcgct ctctttcttt ttctgcttct ttgcgggcca tttcttgtcg
     1321 tgcaaattct atttcctgtt ttttgatttc cgctttcagc tcggcttctt tttttgccag
     1381 gtctgctttt ttatcactca gcgttttttc ataagtggtg aattgctgat caaaacgttt
     1441 ccgcgccatt tcgtaatcct tgccgtcgaa cgcaggataa acaacaactg ttgaggtgtt
     1501 gggttttccg ttttgtataa cggttagatc gaggtaataa ctgatccctt ttgatttttc
     1561 acgcaggcgg acatcgttcc atgttttgga aaaccatgtg cgatcgaaat tttttgattc
     1621 ttcgcccact tcaaatcggg tggatttaaa gctcgccatc tccgggaatt cctcggggtc
     1681 gacattcacc agaaaattgt atttgtcggg attgagtttg cggggttcaa caggcttgct
     1741 ttcttcggtt ttcgccactt cttttttggc ctgttcaacc attccggttt ttttgatgac
     1801 ttcaggatga gattggacag aatgatgagt tgttatttct tcatttgtgt tcacctcttt
     1861 tacatctgta atcacctctt ttacatcttc attctcattt acaattgctt cgtttttccc
     1921 ttttcccaga tatacccaat tgcgggcggc ggtatccaat tcgtaaaaat taaaacggcc
     1981 ttctgcactg gtcgattgca tttttacgct gatcggtttt ttctcgtcga ggtaaacttt
     2041 ttttccgttt tgcacgcaat gaatatcgaa cataccagct gtttcaaaag cataacgtgt
     2101 tcctgcagaa tcgtaggtca tgggtatgcc actaagcagg atttcaggta cagtgtggta
     2161 ttcgcggtac tgcagttctg cttttccttg tatcactttt ccggattcat cggtgagtga
     2221 attggcgggg atgcggatct ctgttcctgt actgtgtttt agaactgctc ctttttgatt
     2281 gtcgaccgta tacgtgctgg cctgcaattc tgctccgggt acaggtggtt ttatacaggg
     2341 agaatcttcg ctgtaatagc cgctgcccaa taaagcattc gccggaatac tatcgctttt
     2401 tacttcgctg atcagggctt cttcctgctg atcttcggtt ggaggatgtg cggcaaaaat
     2461 ggcaacaact gcaatggcag ccatagtgcc tgtaataaca gcagatgaaa taaaccatgt
     2521 cgatttaaaa aatggttttt gcatcagcgc cacctgggaa acgaccttgc ggaagtcctt
     2581 tcgcgactgg atctcctctg aggataccgg ttccctgtcg atgataattt taggtttcat
     2641 tattttggaa tataagtctt aaacattttt ttcagcttct ccagcgagcg gtgacctttt
     2701 accttggcat tgttttccgt aatacccaga atgtcgccga tctctctaaa agaacgtttt
     2761 tcaaaaaatc gccattcaat catttgcagg tcttcctcgg gtaattccgg taaaaccttc
     2821 tccagcaatc ggaacatttc ttcggaatga ccttcctcta tttcttcgag gaattcactc
     2881 acctgacgtg tctcaatgct cacaacccga cgtgtctgat ggctgcgtaa tgcctcatat
     2941 acctcgtttt gcgcaatccg gtagagccag cttgcaaaag gcactcctct aaactggtac
     3001 ttaccgatgt tggtcagtgc tttgaggaac acctgtgagc agagatcaaa ggccgtgtct
     3061 ttgtcatcga tgcgctgatg gatgtagcgg aagatctgtt cgtaatacgc attgtacaga
     3121 ggctcaaacc gcgcgggatc gcgtttggct gcttcaacta cctggagttc atcctgtagt
     3181 tgtcgctctg ttttatggta tctcgaaccg gtctccaaca tgctgattaa tggctgtttt
     3241 gactctataa tgaaagcccg caagaaagtt acaggttatt tctgtctttt ttttccgaac
     3301 gcttgaacag gccgatttgt accatttcat gaatcatgcc gttattagag ctgatttgtg
     3361 cttgatgaca actgttacaa aggaccatca ggcacaaaac acagtcattt ttagtatctt
     3421 tagtaccatg atcagaacac tatttctcct tctaaccatt gttgcttttg ctacgaacgc
     3481 acgggcacag gacaccacca aactggatat tgccgacaat ggggattatt acagtttcag
     3541 aatgctggat aatgtgatca ataattacag gatcatattt acgggcgaaa accatgtatt
     3601 cagaaaaagc aattaccggc tggagctgaa aatgatgaaa tacctttatc acaaagccgg
     3661 atttcgtcac tttattatgg aattcggttt cagcagaggc tggctggtcg ataaatatgt
     3721 gcagaccggc gatacttctt tgtttaaagt attgagcgat tattcgtatc aggaatatgc
     3781 actgctttac aaaggacttt atgaattcaa caagacctta ccagatacgg caaaaatcag
     3841 aattcactcc atcgatattg aacgatcgtt gagtacgcca cttaaggtaa tggatctgct
     3901 gattccaaaa aacaggaaaa taccggcttc tattgcatta acaatcgaat ccattcactc
     3961 gcttgcggtg atgtcggacg atgagcaacg caaggcacaa caaaaaaaca aggaagattt
     4021 ccggacgggt gctccgtttt cactcaagcg ttcgctcaaa tttattttgc aggatctgga
     4081 tagcaacaaa gctgcattta aggaattgct tgggaacgat tatccggtgt tttacaaaat
     4141 cgaacaaggg ttgcgcgctg agatggatcg ttcaacgtac aacgatatga gcgctgtaca
     4201 gggctatttg taccgtgagc gatttatgca tgaaagtttg agagagttgg ctgctgaatt
     4261 tccaacagga aaattttacg gacaatttgg tcgttgccac acttcattgg agacggaaga
     4321 tgtttggtgt ggattttatg cctttaccac tcttgcaact cgtttggata gcgactcgct
     4381 gtttaaaggg aaagtattgg caattgcaca atattatccc aatggcagcg cacatgaaaa
     4441 gggagtgatc gatcgtgacg gggtaaaacc attgctgaaa atttcgagtc ccgatggact
     4501 caccctcatc catcaccgcg atgtggtgaa cgatactgca cgtttgaaaa atcgttttca
     4561 atacctgatc atcaaccagg ccgatcatcg catggataac atgaaagtag acagtaccaa
     4621 accacctgtt gattacagta cctatgaatc gaccatcttt cttgaaggac attacggatt
     4681 acttggtgta aatctgaatg cattgaacag tacgttgttt ggattggatc cttctgcaat
     4741 ttcatttacc tctcccttaa cagttgttgg aggatcgtac acgcaattag aggatggtgt
     4801 attttataca ttcatgtatt ctgcaatacg caatactgaa attggatttt cagattccct
     4861 ttattacgga ttaaaaggcg gttcatttcg ttttcgtgtg ggaggagatt ttgttcgaag
     4921 caaatggctg gatgttggtc cgtttttcgg ttatggatac cagcgattgg tactcaccag
     4981 agatgaaata ctggcaggac cggtaggctc cggatttttc ggtgcaacaa atcgtttgcg
     5041 gacaagtgta accaatccat c
//
LOCUS       DAOMYG010000235         5047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5730_length_5047_cov_13.974760, whole genome shotgun sequence.
ACCESSION   DAOMYG010000235 DAOMYG010000000
VERSION     DAOMYG010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5730_length_5047_cov_13.974760"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2745
                     /gene="infB"
                     /locus_tag="PK610_08405"
     CDS             <1..2745
                     /gene="infB"
                     /locus_tag="PK610_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRJ35996.1"
                     /translation="RLTKVARELNVGLATIVEFLNKKGYKVENNPNAKIDDEIYGVLL
                     AEYQTEKSEKEKSRKVTSSTKEKRETVTITEVKRPTRDQDAEPEEEIMIKNVPVKNEP
                     EVVKPKVESDVKVSVIGKIDLGTVGKKKTTKKAAEPEVKKEKEKEKEVEKPVEKPVAK
                     KTTAKKTEEPAPEPVAEAPVAVETPVVVETPVETPVKEEAIPVVPEIETHKTEYSKLS
                     GPKIVGKIDLPVARDNQSSSSAGERKKRKRIKKVNIDQAAKQQTQRDNQKKRPERNAP
                     KPEVTAEDIQKEIKETLARLSSQGGKSKSSKIRREKRDIRTQLREEEMIRAAEEEKVL
                     KLTEFVTVSDLAKMMNASPTQIISACMTLGIFASINQRLDAETIAIIAEEFGYQVQFV
                     TADIQESIDEDDQDDPEDLVSRSPIVTVMGHVDHGKTSLLDYVRKANVIAGEAGGITQ
                     HIGAYAVQLDNGKKITFLDTPGHEAFTAMRARGAKVTDIAIIVIAADDSVMPQTKEAI
                     AHAQAAGVPMIFALNKIDKPEAKPDKIREDLANMDLLVEEWGGKFQSQEISAKKGLGV
                     KELLEKVLLEAELLDLKANPNRRAKGTVIESMLDKGRGYVSTILVSSGTLEVGDVLLA
                     GSYSGRVKAMFNERGSAIAKAGPSTPVSILGLDGAPNAGDLFNVMDDEREARNIATRR
                     QQLLREQGMRTHKHITLDEIGRRLAIGDFKELNIIIKGDVDGSVEALADSLLKLSTDR
                     IQVKIVHKGVGAISESDVLLASASNAVIIGFQVRPSAAARRLAETEEIDVRLYSIIYK
                     AIEEVKDAMEGMLSPEKQEKIIGTVEVRQVFRITKVGTVAGSYVQDGKITRQSKVRVI
                     RDGIVIHDGTLNQLKRFKDDVKEATAGYECGLSINGFNDINEGDIIEAYEEVEVKSKL
                     "
     gene            2856..3434
                     /locus_tag="PK610_08410"
     CDS             2856..3434
                     /locus_tag="PK610_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35997.1"
                     /translation="MSNFSAWWNSISTFEQTLWAIALGFSLFFLLQTIFTAFGGDTDS
                     ESAMGDAEDLGDGIDFQFLSLKNVVAFFTMFGWVALACHKAGLHPALTISLGTIAGVA
                     MVALMMVLFRSMAKLKHSGTLKTANAIGHTGEVYLTIPAGKGGQGKITILLQGTMREL
                     SAVTDEAQDIPTGKLVKVTGLFNEHVLIVAPV"
     gene            3473..5035
                     /locus_tag="PK610_08415"
     CDS             3473..5035
                     /locus_tag="PK610_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002663122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRJ35998.1"
                     /translation="MSEYVLIAIGVAVLFFFILLSAIFRRYKRCPSDQILVVYGKVGR
                     GKDGDRTAKCIHGGAAFIWPVFQDYAFMELTPMSIEVNLTNALSRQNIRVDVPSKFTV
                     GISTDPGIMENAAERLLGLGRADIHSLANDIILGQMRLVVAMMDIEEINSNRDKFLAN
                     VASNVEAELKKIGLKLINVNITDIKDESGYIEALGKEAAAKAINEAKKSVAEQERMGE
                     VGKAIAERERDVSMQQTQKERDVQIAETQRDRDTSIASAIKDREILIAQAKRDEQIGK
                     VEADRDTRIKSAEANALAVTGENTSRIQIAQSEAARREREAEALRIAIAAEKVQQAKA
                     LEEAYTAERNAEAARAERERASQNANIVVSAEIAKQKAIIEAQAEAEKIRERAKGEAD
                     AIYAKLEAEARGVYEMLTKQAEGMNALVNAAGGNSKNAALLMIADKLPELVKLQSEAI
                     QNIKIDKVTVWDGGQNSEGKGSTANFVSGLYKSVPPLKDIFDMAGLDIPTYLGKEKGK
                     NDNDIVDAEEAE"
BASE COUNT         1579 a         1132 c         1210 g         1126 t
ORIGIN      
        1 cgcctgacga aagtcgcacg agaattaaac gtgggactgg cgaccattgt agaatttctg
       61 aacaagaaag gctataaggt agaaaacaac cccaatgcca agatcgatga tgagatctat
      121 ggagtgttgc ttgccgaata ccagaccgag aaatcggaaa aggaaaaatc gcgcaaggta
      181 accagcagca ccaaagagaa gcgggaaacc gttaccatca ccgaagtgaa acgtccaacc
      241 cgcgatcagg atgctgagcc ggaagaggag atcatgatta aaaacgttcc ggttaaaaac
      301 gaaccggaag ttgttaagcc aaaagtggaa tccgatgtaa aggtttctgt aattggaaaa
      361 attgatctgg gaacagttgg taaaaagaag accaccaaaa aggcagcgga acctgaggtt
      421 aaaaaagaga aggaaaaaga aaaagaagta gaaaagcccg ttgaaaaacc ggttgccaaa
      481 aaaacaactg ccaagaaaac agaagaaccc gcgccggaac cggttgctga agctcctgtt
      541 gcagttgaaa cacctgttgt agttgaaact cccgttgaaa ctccggtaaa agaggaagca
      601 atacctgttg ttccggaaat tgaaacacac aaaacggaat acagcaaact gagcggacca
      661 aaaatcgttg gtaagatcga tcttcctgtt gcccgcgata atcaaagttc ttcttcggca
      721 ggtgagcgta aaaaacgcaa acgcattaag aaggtcaaca tcgaccaggc ggcaaagcaa
      781 caaacgcagc gcgacaatca gaaaaaacgt cccgaacgca atgcgccaaa accggaagtc
      841 acagcagaag atattcagaa ggaaatcaaa gaaactcttg cccgcttaag cagtcaggga
      901 ggaaaatcaa agtcctcgaa aattcgccgc gaaaagagag atatccggac tcagttacgc
      961 gaagaagaaa tgattcgtgc ggccgaagaa gaaaaagtgt tgaagcttac cgaattcgta
     1021 acggtttctg accttgcaaa aatgatgaat gcttcaccca cgcagatcat ctccgcctgt
     1081 atgacactcg gaatttttgc atcgatcaac cagcgtctcg atgcagaaac cattgcaatc
     1141 atcgcagaag aattcggtta ccaggtacaa tttgttactg ccgatattca ggaatcgatt
     1201 gatgaagacg atcaggatga tcccgaagat ctggtttctc gttctccgat tgtaactgta
     1261 atgggtcacg tcgaccatgg taaaacatca ttgctcgact atgtccgtaa agccaacgta
     1321 attgccggtg aggccggagg tattacacag cacatcggtg cctacgccgt gcagctcgat
     1381 aatggcaaaa aaatcacctt cctcgatact ccgggtcacg aagcgtttac cgcaatgcgt
     1441 gcacgtggag ccaaggtaac cgacatcgcc atcattgtaa ttgcagcaga cgactctgtg
     1501 atgccacaaa ccaaggaggc aatcgcacac gcacaagcgg caggtgtacc tatgattttt
     1561 gcgctcaata aaattgataa gccggaagcc aaacccgata aaatccgtga agaccttgcc
     1621 aatatggacc tcctcgttga agaatggggc gggaaattcc agagtcagga aatctcagcg
     1681 aaaaaaggtt taggtgtaaa agaactcctc gaaaaagtat tgctcgaagc agaattgctc
     1741 gatttaaaag caaatccgaa tcgcagagca aaaggaacag tcatcgaatc gatgctggat
     1801 aaaggacgcg gatacgtatc tacaatcctt gtttcttccg gtacactcga agttggagat
     1861 gtattgcttg caggatccta cagtggtcgt gttaaggcga tgttcaacga acgcggttca
     1921 gccattgcaa aagcaggtcc ttctactccg gtttccatcc ttggattgga cggtgcacca
     1981 aatgcaggtg acctcttcaa tgtaatggat gatgaacgcg aagcaagaaa tattgcaacc
     2041 cgtcgtcagc aactcttgcg cgaacaggga atgcgtacgc ataaacatat tacgctcgac
     2101 gaaattggtc gtcgtctcgc aatcggcgac ttcaaggaac ttaacatcat cattaaaggt
     2161 gacgttgacg gatctgtaga agcacttgcc gattctttgc tcaaactttc taccgaccgt
     2221 atccaggtga agatcgttca caaaggagtt ggtgctatca gtgaaagtga tgtattgctt
     2281 gcatcggcat ccaacgctgt tatcatcggt ttccaggttc gtccatcagc agccgctcgt
     2341 cgtcttgccg aaacggaaga aatcgatgta cgcctctatt ccatcattta caaagccatc
     2401 gaagaagtaa aagacgcgat ggaaggaatg ctctctccgg agaagcagga aaaaattatt
     2461 ggaacagtgg aagtgcgtca ggtatttcgt atcaccaaag tgggaaccgt tgcaggttca
     2521 tacgtgcagg atggaaagat cacacgtcaa tccaaagtac gcgttatccg cgatggtatt
     2581 gttattcacg atggcacact caaccagctt aagcgtttca aggacgatgt gaaagaagca
     2641 acagcaggat acgaatgcgg attgagcatt aacggcttta acgacattaa cgaaggcgac
     2701 atcattgaag cttacgaaga agtagaagtg aagagcaagc tctgatcact gagcctgatc
     2761 cttcgttgat ttccttctca ctgttgaaaa cctgcagggc ttttgctgta acttagtacg
     2821 acccctttcg tattacttgc tgctaaacca atttcatgag taatttttcc gcctggtgga
     2881 attccatttc cactttcgaa cagacactct gggcaatcgc actgggattc tcactgttct
     2941 ttttactgca aaccatcttc accgcttttg gtggcgacac cgattccgaa tcggccatgg
     3001 gtgatgccga agatctggga gacggtatcg attttcaatt tctgtcactg aaaaatgtag
     3061 ttgccttttt caccatgttc ggttgggttg cgcttgcttg tcataaagca ggattgcatc
     3121 cggcactaac gatctccctg ggaacaattg caggtgttgc aatggtagct cttatgatgg
     3181 tgcttttccg ctcaatggcg aaacttaaac acagcggaac actcaaaacc gcaaacgcca
     3241 tcggacatac cggcgaagtg tacctcacta ttcctgccgg aaaaggcgga caaggaaaaa
     3301 ttaccatttt gctgcaaggc accatgcgcg aactgagtgc ggttactgat gaagcgcagg
     3361 atattcccac cgggaaactt gtaaaagtta cgggactttt caacgaacat gtcctgattg
     3421 ttgctccggt ttgattttta gtctatattt tcacacataa atctttttta ctatgtcaga
     3481 atacgttttg attgccattg gcgtcgcagt tctcttcttc ttcattcttc tttctgcgat
     3541 ttttcgtcgc tacaaacgtt gtccatccga ccagattctt gttgtgtatg gtaaggtggg
     3601 tcgcggaaaa gacggagacc gaactgcaaa atgtattcat ggtggagccg cattcatctg
     3661 gcccgtattc caggattacg ccttcatgga acttacgcca atgtcgatcg aggtgaatct
     3721 gaccaatgcg ctttcgcgtc agaatatccg cgtggatgtt ccgtcaaaat ttacggttgg
     3781 tatctccacc gatcccggca tcatggaaaa tgcagcagag cgtctgctgg gattaggtcg
     3841 tgccgatatt cattcgcttg ccaatgatat tatacttggt cagatgcgtc tggttgttgc
     3901 catgatggac atcgaagaga tcaactccaa ccgcgataaa tttcttgcca acgttgcatc
     3961 gaatgtggaa gccgaattga aaaaaatcgg tttgaaactc atcaacgtaa acatcaccga
     4021 catcaaagac gagagcggat acattgaagc actcggtaaa gaagccgcag caaaagccat
     4081 caacgaagca aagaaatcag ttgctgagca ggagcgtatg ggtgaagtgg gtaaagccat
     4141 cgccgaacgt gagcgtgatg taagcatgca gcaaacccaa aaggaacgcg acgtacaaat
     4201 tgcagaaaca caacgcgaca gagatacttc gattgccagc gcgattaaag acagagaaat
     4261 tttaattgca caggcaaaac gtgatgaaca aattggtaaa gtggaagccg acagagacac
     4321 ccgtatcaaa tcggcggaag caaatgcatt agcagtaacc ggagaaaaca cctcacgcat
     4381 tcagatcgcc caatccgaag ctgcacgaag agaacgtgaa gcagaagcgc ttcgcattgc
     4441 aatcgcagca gaaaaagtgc aacaggcaaa agccctcgaa gaagcctata ccgcagaacg
     4501 aaatgcagaa gcagcccgtg cagaacgcga acgtgcatca caaaatgcca acatcgttgt
     4561 aagcgcagaa atagcaaagc aaaaagccat catcgaagca caggccgaag cagaaaaaat
     4621 acgtgagcgc gccaagggtg aagccgatgc gatttacgcc aaactcgaag cagaagcacg
     4681 tggtgtatac gaaatgctca ccaaacaggc cgaggggatg aatgcccttg taaatgcagc
     4741 cggaggtaat tccaaaaatg cggccttgct gatgattgcc gataaattac ctgaactggt
     4801 gaaactgcaa tccgaagcga ttcaaaatat caagattgat aaagtaaccg tttgggacgg
     4861 tggccagaac agtgaaggaa aaggatcaac tgcaaacttc gtttcaggtt tgtacaaatc
     4921 tgttccaccg ctcaaagata tttttgacat ggccggtctg gatattccta cttacctggg
     4981 aaaagaaaaa gggaaaaacg ataacgatat tgttgacgcg gaagaagcgg aataatcttc
     5041 tccgaat
//
LOCUS       DAOMYG010000236         5043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5741_length_5043_cov_6.709503, whole genome shotgun sequence.
ACCESSION   DAOMYG010000236 DAOMYG010000000
VERSION     DAOMYG010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5741_length_5043_cov_6.709503"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PK610_08420"
     CDS             <1..669
                     /locus_tag="PK610_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ35999.1"
                     /translation="LTLKIRIRGNSDVFFNGKIVGFSDRINTILVPLDFLRYSNLNFQ
                     TRQPTSPSRLILAVTDLSHPGIARMMEEKGYDTNQENLKGSRVKTILLVVLVLFLFIG
                     GVIVVLSILTFIQYGQILISRVNYELRVLLLMGYDHTKLANHYLGYVMIWLAIIFLSA
                     IGLLYLAEIELLKYLKEYELSTQPGISLYVWLAAFGLILVYTAINAWSIRINLRRLAK
                     NLQG"
     gene            747..2207
                     /locus_tag="PK610_08425"
     CDS             747..2207
                     /locus_tag="PK610_08425"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRJ36000.1"
                     /translation="MKKLLFLLALITTSGFSLKAQNPLLKFRINGIKDTTVHLAYYFG
                     NKLYYSDTSRADKTGTVIFGKKKTYEPGVYAVVIPGTRYFEIVLNNEPVEMNTDTSDL
                     NGKMVVVKSKENKLFYDYIQFINARRLESEPHREKMKTADSKSKEFEDAKAKLAQVDK
                     KVKDHQAAMFKDNPTSLTAKIIRLSTDPEIPEPPKNADGSLKDSLFAYKYAKAHYWDH
                     LDFSDPRLVRCPFYHNRLESYFKNMIAQVPDTMIVEADRLISKMLDNKDMFKYTVHYL
                     TYTFESSKQMCMDAAFVHLAFKYYKTGKAFWLEEDKVKKVIDRAEELAPILCNVAVHP
                     LSMPDTTMKWRRLYDVKAQFTILIFWDPECGHCKKEMPKFAEMYAKLKAQGVEIWAVS
                     SDRNDKWFKFIRDNKMNFINVAIPEEVYSNQELAKEWLGKGYTDLKSLNYRTAFDIYS
                     TPKVFLLDKDKKILAKQIDADQIEKLIEFYRNKEKK"
     gene            complement(2271..2552)
                     /locus_tag="PK610_08430"
     CDS             complement(2271..2552)
                     /locus_tag="PK610_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36001.1"
                     /translation="MSKTFGIVAGTIVVILALLMILQLWGVIHIGFLSFIKSGATLLI
                     LLGALIALFVIYGMFIWKGSDLSLREQRELAKKNQSQSNDVQSGRSSDQ"
     gene            complement(2598..3083)
                     /locus_tag="PK610_08435"
     CDS             complement(2598..3083)
                     /locus_tag="PK610_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRJ36002.1"
                     /translation="MEPRRSDNFYKWTSHSVYPVSLGLPASMLIRGYSSNNEELIKAG
                     WVQTGSLLLCTGLSQGLKIGIRRERPYKAHPGLFRAKLKTGPFSFPSGHSATAFASAT
                     SLCLAYPRWYVIAPSMLWAGTVAWSRMHLGVHYAGDVLGGLVIGVGSSLIMWGIGKAT
                     D"
     gene            3227..3787
                     /locus_tag="PK610_08440"
     CDS             3227..3787
                     /locus_tag="PK610_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018945477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HRJ36003.1"
                     /translation="MKSLLLLYLFLLPSKEEKFKTIIQHAKNQTLKEVTYDPSYKSIP
                     YPNGDVPEHLGVCTDVIIRAYRSIGIDLQKLIHEDMKIAHSEYNKRRKTDKLDTNIDH
                     RRTPNMETFLVRKKAGKPITDKGADYEPGDIVFWAVATGHVGLVVDEKVPGTDRYYIV
                     HNICCGPKKEDFLFEAKITGHYRWMP"
     gene            complement(3840..4700)
                     /locus_tag="PK610_08445"
     CDS             complement(3840..4700)
                     /locus_tag="PK610_08445"
                     /inference="COORDINATES: protein motif:HMM:NF006133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ36004.1"
                     /translation="MHVPFSYLHKKRSMEFKGKTVLISGGSRGIGKAIALHLGKSGAN
                     VVIAAKTAEPHPKLEGTIYSAAEDIIKAGGQAHAVMCDIREEDQVINAVEETVKTFGG
                     IDIVINNASAINLSPTSTLQMKTYDLMMDINVRGTFLFTKTALPHLKNSTHPHILTLS
                     PPLNLDPKWFSKHVAYTMSKYGMSMAALGFSEEYKKFGIASNSLWPRTTIATAAIMNI
                     VGGEQMMKMSRHPEIMAEAAHEILLRDPKVFTGNFCIDEDILKEKGISNFDQYRVDPN
                     TPLMTDLFLD"
     gene            4741..5025
                     /locus_tag="PK610_08450"
     CDS             4741..5025
                     /locus_tag="PK610_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ36005.1"
                     /translation="MNSSLRPMFVIPDLLRSGLWGWSWNRTSWPGKLKADDPANKKKL
                     IALQDSLLELPGAGHFLNRARFYTSLGEIEKALADYNQVIHEDPVPKPRR"
BASE COUNT         1494 a         1023 c         1089 g         1437 t
ORIGIN      
        1 ttgacactta aaatcagaat cagaggaaat tcagatgttt tctttaacgg taaaattgtt
       61 ggtttctctg atcgtatcaa taccattctt gtaccgcttg attttctgcg gtattccaat
      121 ctcaattttc aaacacgcca accaacttca ccttcgcgcc ttattctggc agttaccgat
      181 ctttcccacc cgggaattgc ccgcatgatg gaggaaaagg gttacgatac caatcaggaa
      241 aatctgaaag gaagcagggt taaaactatt cttctggtgg tattggttct ctttcttttt
      301 attgggggag taattgtcgt actctccatt ctcacattta tccaatacgg tcagatcctg
      361 atcagcaggg tcaattacga gctcagggtt cttttactta tggggtacga tcataccaaa
      421 ctggcaaacc actatctggg atatgttatg atttggctgg caataatatt tttaagtgcc
      481 ataggattgt tgtacctggc tgaaatagaa ttgttaaaat accttaaaga atacgaattg
      541 agcacacaac cggggatctc gctttacgtc tggttggcag cttttggact gattcttgtt
      601 tatactgcca tcaatgcctg gagcattcgt ataaaccttc gccgattggc aaagaacttg
      661 cagggttagg gttaatgatt tgttaaaggg tagtataaca tcgtttcatc ctttaaattc
      721 gaactgtaaa acatcgtgat atgaaaatga aaaagttact ttttctgctg gcactgatta
      781 ccacctccgg tttttctctt aaagcacaga atccactcct taaatttcgc atcaacggaa
      841 ttaaggacac tacagtacat ctggcttatt atttcggaaa taagttgtac tacagcgata
      901 catccagagc cgataaaaca gggaccgtta tttttggaaa gaagaaaact tatgaacccg
      961 gagtttatgc cgtggtaatt cccggtaccc gctattttga aattgtgctg aacaatgagc
     1021 cggttgaaat gaataccgat acttccgatc taaacgggaa aatggttgtg gtaaaatcaa
     1081 aggagaacaa gctgttttac gattacattc agttcatcaa tgcccgccgc ctggagtcag
     1141 aaccgcatcg tgaaaaaatg aaaacagccg attcaaaaag taaggaattc gaagacgcta
     1201 aggcaaaact ggcacaggtc gataagaaag ttaaggatca ccaggctgca atgtttaagg
     1261 ataacccaac ttcgcttact gcaaaaatca ttcgcctttc aaccgatcct gaaattcctg
     1321 aaccgccaaa aaatgctgat ggttcgttaa aagattcttt gttcgcctat aaatatgcga
     1381 aagcacatta ttgggatcac ctcgatttct cggatccgcg attagtgcgt tgcccgttct
     1441 atcataaccg actggaaagc tattttaaaa acatgattgc tcaggtcccc gatacaatga
     1501 ttgtggaggc cgatcgtttg atttcgaaaa tgctcgataa taaagacatg tttaagtaca
     1561 ctgttcatta cctcacttat acatttgagt cgagcaagca aatgtgtatg gatgctgcct
     1621 ttgtgcatct ggcatttaaa tattacaaaa caggaaaagc attctggctg gaagaagata
     1681 aggtgaaaaa agtgatcgat cgtgcagaag aacttgcacc cattctttgc aatgttgccg
     1741 tacacccgct ttccatgccg gatactacaa tgaaatggcg gcgtctctac gatgttaagg
     1801 ctcagtttac cattttgatt ttctgggatc cggaatgtgg tcattgtaaa aaagaaatgc
     1861 caaaattcgc agaaatgtat gcgaaactga aggcacaggg agttgaaatt tgggcagtaa
     1921 gctccgatcg caacgataaa tggttcaagt ttattcgcga taacaaaatg aactttatta
     1981 atgtagcaat tcctgaagag gtctattcca atcaggagct ggccaaagaa tggttgggca
     2041 aagggtatac agatttaaaa tccctgaact accgcacggc ttttgatatt tacagtactc
     2101 ctaaagtatt tcttttagat aaggacaaga agatccttgc aaagcaaatt gatgctgacc
     2161 agatcgaaaa actgatcgag ttttatcgga ataaagagaa gaagtaataa ttcttccaac
     2221 aaaaaaagcc cctgcgaatc gctggggctt ttttgtttta gcttttattt ttattgatca
     2281 gaacttctgc ctgactgaac atcgttggat tggctttgat tctttttggc taattctctt
     2341 tgttcgcgca atgaaaggtc gctccctttc caaatgaaca tgccgtaaat aacaaacaga
     2401 gcaatcaagg ctccgagaag gataagtagt gttgcgccac ttttaataaa gctgaggaat
     2461 ccaatatgaa taacgcccca taattgaagg atcattaaca gtgcaaggat cacaacgatg
     2521 gttcctgcta caattccgaa tgttttgctc attttgattg gttttgttgg atgtctacga
     2581 atatcgtaaa aaaatgttta gtctgtcgct ttcccgattc cccacatgat caacgatgaa
     2641 cccacgccaa ttaccagtcc acccaggaca tcccccgcat aatgtacgcc caaatgcatt
     2701 ctcgaccagg ctactgttcc cgcccaaagc atggatggtg caattacata ccaacgggga
     2761 taagccaaac agagacttgt agcgcttgca aaggcagtgg ctgaatggcc ggaagggaaa
     2821 ctgaatggtc cggtttttaa ttttgccctg aacagcccgg gatgtgcttt ataggggcgt
     2881 tcacgacgga tgcctatttt cagaccttga ctcaatccgg tacaaagcaa cagcgaaccg
     2941 gtttgtaccc agcccgcttt tatgagttct tcgttgttgg aggaatagcc tctgatcagc
     3001 atggatgcag gaagtcctaa cgaaaccgga tatacactat gggatgtcca tttgtagaaa
     3061 ttgtcagatc tacggggttc catggtatgt attttgctta ataaatccac acccagggat
     3121 tgtcccgcaa cggaattcac caggagcatg aaaaataacg gaattaccac tcgaacggac
     3181 ataacccaaa tgtaaatcat acgtagaacc ctgcaaatac ttttttatga aatcactcct
     3241 tctgttgtat ctgtttttgc ttccttccaa agaagaaaag tttaagacga ttattcagca
     3301 tgcaaaaaat cagacattaa aagaggtgac ctatgatcct tcgtacaaaa gcattcccta
     3361 tcccaatggt gatgtgcctg aacatttggg ggtttgtacg gacgtaatca tcagggctta
     3421 ccgatccatc ggtatcgatt tacagaaatt gatccatgag gatatgaaaa ttgcccattc
     3481 cgaatacaac aaaagaagga aaacagataa gctggatacc aatatcgacc accgccgtac
     3541 acctaatatg gaaacctttc ttgtgcgcaa aaaggcggga aaaccaatta ccgataaggg
     3601 agcggattac gaaccgggag acattgtttt ttgggcagtg gcaaccggac atgttggttt
     3661 agttgtagat gaaaaagttc cgggtaccga caggtattac attgtgcata atatctgttg
     3721 tggtccgaaa aaggaagatt ttttatttga agcaaaaata accggacact acaggtggat
     3781 gccttgatgg ggagaacagt gagcagttgt cgatgtctga ataagattgg ccggttttat
     3841 taatccagaa acagatctgt cataagcggt gtgttcggat cgacgcggta ctgatcgaaa
     3901 ttgctgatac ctttctcctt taaaatatcc tcatcgatac aaaagttacc agtgaaaact
     3961 ttcggatcgc gaagtaagat ttcatgagca gcttctgcca tgatttcggg gtgacgcgac
     4021 attttcatca tttgttcacc cccaacaata ttcataattg cagcagtggc aatggttgtc
     4081 cttggccaca gtgaattgga tgcaatgcca aattttttgt attcttcact gaatccgaga
     4141 gcagccatac tcatcccgta ttttgacatg gtataagcaa cgtgcttgga aaaccatttt
     4201 ggatcaagat tgagtggggg agagagggtg aggatatgtg gatgagttga attcttcaaa
     4261 tgtggaagcg cagtttttgt aaacaggaaa gtacctctaa cgttgatgtc catcatcaga
     4321 tcatacgttt tcatttgcaa tgttgacgta ggagaaaggt taatggcaga agcattattg
     4381 atcacgatat caatacctcc aaacgttttt acagtttctt caacggcatt aataacttga
     4441 tcttcttcgc ggatatcaca catgacggca tgcgcctgac cacctgcttt aatgatatct
     4501 tcggctgcag aataaatagt tccttcaagt tttgggtgtg gctctgcggt tttggcagcg
     4561 ataacaacgt tggctcctga ttttcccaaa tgcaaggcaa ttgcttttcc gataccacga
     4621 ctaccaccac tgatgagaac tgtttttcct ttgaattcca tacttctttt tttgtgaaga
     4681 taggaaaaag gaacatgcaa tgacgatgga ccttaaggga aggaattgta tttttgatca
     4741 atgaattcct ctctgcgtcc aatgtttgtt attcctgatc ttttgcgttc aggactatgg
     4801 ggatggtcgt ggaacagaac ctcctggccc ggaaaactaa aagcggatga tccagccaat
     4861 aaaaagaagc tcatcgcttt acaggactca ttgcttgaat taccgggtgc cggacatttt
     4921 ttgaacagag cgcggtttta cacctcactt ggggaaattg aaaaggcttt ggccgactat
     4981 aatcaggtca ttcacgaaga tccggtgccg aagccaagac ggtagtgttc tttgccattg
     5041 acg
//
LOCUS       DAOMYG010000237         5015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5791_length_5015_cov_8.303226, whole genome shotgun sequence.
ACCESSION   DAOMYG010000237 DAOMYG010000000
VERSION     DAOMYG010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5791_length_5015_cov_8.303226"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PK610_08455"
     CDS             complement(<1..397)
                     /locus_tag="PK610_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRJ36006.1"
                     /translation="MHALSNLRGSIVAIVTPFTADGSIDFQSYANLIKRQIEGGTNGI
                     VVAGTTGESAALTEDEYAALIRFTVQQVNGQVPVIVGAGSNVTARAIRHSQVAQREGA
                     DALLILNPYYNKPTEEGIFLHYKSIAENVN"
     gene            496..1155
                     /locus_tag="PK610_08460"
     CDS             496..1155
                     /locus_tag="PK610_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRJ36007.1"
                     /translation="MTTIPEVVEDIIRKSPFLEEALADGLINVSSLARKIRSEISERL
                     KKDVKEGAVIMAINRMAPSNYYKINLKIQNFMHSIGDVIVRSNLSDFTFTNSETLYVA
                     TADLLKEAGQHKELFCTFSAGVYETTVIVSDSLKDVVNRSFHKEKQISFSENLASITI
                     KLPTENTEISGIYYYILKRIAWEGINIREVISTSNEFTLVVNDVDIDRTFSILMSLKK
                     M"
     gene            1249..3684
                     /gene="gldG"
                     /locus_tag="PK610_08465"
     CDS             1249..3684
                     /gene="gldG"
                     /locus_tag="PK610_08465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HRJ36008.1"
                     /translation="MLALLLKEIRGFLSSLIGYIVISVFLLLMGLFIWVFSTPFNILE
                     SGFADLDPLFNLAPLVFLFLIPAITMRSFAEEKRTGTIELLLTRPLSDVQIILAKYFA
                     GLLLVLFTLLPTLLYYYTINQLGDPKGNVDTGGMWGSYLGLTMLSASFVSIGIFASAI
                     SQNQIISFILGVFLCFFVYLGFDFMGSYSIFGSFDSVIRDLGIYEHYHSISRGVIDSR
                     DVIYFVSVVIVFVLLTRLVLESRKKIHRLQLVINLAIVLVVNILGTFGFFRLDLTQEK
                     RHSLADATISFVENRLEDVITFQIYLTGELPADLKRIEREIKEKLDNMKAYAGDNIQY
                     EFFDPYSIEDEKDRFAFMDQLDYERKIRFSILELEEAGKISQKFLFPGATVSYGTQQS
                     VSFNFFSKQIIGKGENLKDLADATVNSIEYQLMDAMVRVTQPTRKRIAILEGHGEAHN
                     DQLGIIINALGEFYAVERVKIDNKINAIRDFNALLIIQPDSVFSEKDKFVIDQFIMKG
                     GKVAWFLDAMDVQRDTLMLRGQTFGIARDLNLDDQLFTYGVRINKEILLDYQCAPYAV
                     GPGGVHEWYFYPLLKPERDHPITQSIDPIKTEYANTIDLVGNDPEQRKSILLKSSERS
                     TAYKSPARINKGVIDLKDKLFNQKTNGKPVAVLLEGQFNSVFKDRLPPEFMNSPDYVF
                     KDRSVKSRMLIVADGDIPVNEVDYQEVNGQTVPRYRKLHSDKYNIRNPDGSPRFIYGN
                     KEFVLNAVDYLMGDTSLIDIRQRTVMIRRLNEDRVISERKYWQMVNVGLPILLIIAFG
                     IGQNYYRRRKYTR"
     gene            3697..4743
                     /locus_tag="PK610_08470"
     CDS             3697..4743
                     /locus_tag="PK610_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36009.1"
                     /translation="MKRRQLQLLISAAVLITLSVVVVYMKQKKSNSTFTKDLSDFTIE
                     DTASIGKLKITNQDKISAELVRKHAGYWELNGKFRARVDAIDLMLTTAKKVRVKNPVP
                     ESARENIIRNLSVNYHKVEYFDLNGKAIKTWYVGHATKDHDASYMLLETPSEGKSSLP
                     YVTYIPGFAGELSSRYFCVEEEWRFTGIFNYDPSEIIAVSIRNNDYPEESFEVKVLDK
                     NLFALHDQFGKKVERFDTAGVRQLVVNFKKIHFETLNRGILSPLQEDSLRKAKPYYEI
                     SVTDKKGEKKEIKVFHKKLPPHNMNNEPFEQDYPYDPERAFAQIPGGEIVVIQFFVFD
                     KILWPIDAFLPRKE"
     gene            4874..>5015
                     /locus_tag="PK610_08475"
     CDS             4874..>5015
                     /locus_tag="PK610_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRJ36010.1"
                     /translation="MKFIVSSTALLKQLQSISGVLNSSNTLPILDNFLFEINKKQLTV
                     SAS"
BASE COUNT         1440 a         1061 c         1036 g         1478 t
ORIGIN      
        1 ggtttacatt ttctgcgatt gacttgtaat gcaaaaagat tccttcttca gtaggtttgt
       61 tgtagtaagg attcaaaatc agaagagcat cagcaccttc acgttgagcg acctgtgaat
      121 gacggattgc ccgggcagta acattactac ctgcgcccac aatcacaggg acctgaccat
      181 taacctgttg aacagtaaat cggataagag ccgcatattc atcttctgtt aatgctgcac
      241 tttcaccggt tgttccggca acgacgattc cgttggtacc accctcgatt tgacgtttaa
      301 ttaagttggc ataggattga aaatcaatac ttccatcagc cgtaaaaggt gttacgatgg
      361 ctacaataga tccgcggagg ttgcttaaag cgtgcattat ttcttttttt tagcaaaact
      421 aaacatagac ttgcattgtt atgaaatttt acttacattt acacttacat ttaacactgt
      481 gtaataaatt ttaacatgac taccattcca gaagtcgtag aagatatcat ccggaaatcc
      541 ccttttctgg aagaagcttt ggcagatggg ctgatcaatg tctcctcatt ggcccgaaag
      601 attcgttctg agatctctga gcgtttaaaa aaggatgtta aagaaggtgc tgttattatg
      661 gccattaacc gcatggcgcc cagcaactat tacaagatca atctgaaaat acagaacttc
      721 atgcattcca ttggggatgt aattgtgcgt tccaaccttt ccgatttcac gtttaccaat
      781 tccgaaacat tatatgtggc aaccgcagat cttcttaaag aggcgggtca gcacaaggaa
      841 ttgttctgca ctttttcagc cggggtttac gaaaccacgg tgattgtgag cgattcattg
      901 aaggacgtgg tgaacaggag ttttcataag gaaaagcaga tttcgttttc cgaaaacctc
      961 gcttccatta ccattaagct ccccaccgaa aacactgaaa tctctggaat ctattactat
     1021 atcctcaagc gcatcgcctg ggaaggcatt aatattcgcg aggtcatttc cacctccaat
     1081 gagtttaccc tggtggtgaa tgatgtcgat atcgaccgga ctttctcgat tctgatgagt
     1141 ctgaagaaaa tgtgaccttt ctttttgagc attgctccgt tggttaattg ctgttaaagt
     1201 gcgattctcc attcggtgct aatgttattt ttgcttaaaa ctccatccat gctggcattg
     1261 ctgctgaaag aaatcagggg attcctaagt tcacttatcg gttacatcgt aatctcggta
     1321 ttcctgttgt taatgggatt gtttatctgg gtattctcaa ctccctttaa cattctcgaa
     1381 tcagggtttg ccgaccttga tcctcttttc aacctcgctc ctttggtttt tctttttctg
     1441 attcctgcta tcactatgcg cagttttgcg gaagaaaagc gtacaggaac catcgaatta
     1501 ttattgacgc ggcccttatc ggatgtgcag attattcttg cgaaatattt tgcaggatta
     1561 ttattggtgc tgttcacact gcttccaaca ctgctctatt attacaccat caatcagctt
     1621 ggcgatccga aaggaaatgt tgacaccgga ggaatgtggg gctcctatct tggcttaact
     1681 atgttgagcg caagctttgt gtcaatagga atatttgctt ctgctatttc gcaaaatcag
     1741 atcatctcct ttatacttgg agtattcctt tgcttttttg tttacctcgg tttcgacttc
     1801 atgggctcct actccatctt tggatccttc gattctgtta ttcgcgacct gggaatttac
     1861 gagcactacc attcgatcag ccggggtgtt atcgattcgc gtgatgtgat ctatttcgtt
     1921 tctgtggtga ttgtgtttgt actgctcaca cgactagtac ttgaatcaag aaaaaaaatt
     1981 catcgacttc aattggtcat caaccttgca attgttcttg tggtgaatat ccttggaaca
     2041 tttggatttt tccgtcttga cctcacccag gaaaaacgtc actcccttgc agatgcgacc
     2101 attagttttg tagaaaacag acttgaagat gtaataacct ttcagatcta tttaaccggc
     2161 gagttacctg cagatttaaa acgaattgag cgtgagataa aggaaaaact cgacaacatg
     2221 aaagcctatg caggggataa tattcaatat gagtttttcg atccctattc cattgaagat
     2281 gaaaaagacc ggttcgcctt catggatcaa ctcgattatg aacgcaagat ccgtttctca
     2341 atacttgaac tggaagaagc aggtaagatt tcgcaaaaat tcctttttcc cggagccacg
     2401 gtttcttacg gaacacaaca aagcgtttcg tttaatttct tttcgaagca aatcatcggc
     2461 aagggagaaa atctgaaaga cctggcggat gccactgtaa acagtattga ataccagttg
     2521 atggacgcaa tggtacgcgt tactcagccc actcgcaaac gaattgcaat acttgaaggt
     2581 catggcgaag cacataacga tcagttagga attatcatca atgcattggg agaattctat
     2641 gcagtagaac gtgttaagat cgataataaa attaatgcta ttcgcgattt caatgccctg
     2701 ctcatcattc agcccgacag tgtgttttct gagaaggata aatttgtgat agaccaattt
     2761 atcatgaaag gtggaaaagt agcatggttt cttgatgcaa tggatgtgca gcgtgataca
     2821 ctaatgcttc gcggacaaac atttggaata gcgcgcgatc tgaatcttga tgatcaactg
     2881 ttcacctatg gtgttcgtat caataaagaa attttacttg attaccaatg cgcaccctat
     2941 gctgttggcc cgggcggagt gcacgaatgg tatttttatc ctcttttaaa acccgaacgt
     3001 gatcatccca ttacacagag tattgatccg ataaaaacag agtatgcaaa cactatcgat
     3061 ttggttggga acgatcctga acagcgaaaa agtattctgc taaaaagttc ggagcggtct
     3121 actgcctaca aatcacctgc acgaatcaac aagggggtta tcgatttgaa ggacaaactc
     3181 ttcaaccaaa aaacaaacgg caagccagtt gctgttttac tggaaggaca attcaattcc
     3241 gttttcaaag atcgtctccc tcccgaattc atgaatagtc cggactatgt ttttaaagat
     3301 cgctccgtga aaagcagaat gctcattgtt gccgacggag atattcctgt gaacgaagtc
     3361 gactaccagg aagtaaacgg acaaacagta ccacggtacc gtaaacttca ttccgacaaa
     3421 tacaacatcc gcaatccgga tggctctcca cgatttattt acggcaataa agaatttgtg
     3481 ctgaatgcag ttgattacct aatgggcgac acatcactga tcgatattcg tcagcgtact
     3541 gtcatgatcc gtcgtttgaa tgaagatcgg gtgatcagcg aaagaaagta ttggcaaatg
     3601 gtgaatgtgg gacttcccat tttacttatc atagcctttg gaattgggca aaattattac
     3661 cgtcgccgca aatacacgcg ataaaaaatc agcaacatga aaaggagaca acttcaacta
     3721 cttatttcgg cagcagtgtt aatcacgctt tctgttgttg ttgtatacat gaagcaaaag
     3781 aaaagcaaca gtactttcac aaaagacctt agcgatttca ctattgaaga cacagcctcc
     3841 atcggaaaat tgaagattac caaccaggat aagataagcg ccgaactggt tcgtaaacat
     3901 gcaggctatt gggaactgaa cggaaaattc cgtgcacgtg ttgatgctat tgatttaatg
     3961 cttaccaccg caaaaaaagt tcgcgttaaa aatcctgttc ccgaatctgc cagagaaaat
     4021 ataattcgca atctttccgt gaactaccat aaagtagaat attttgacct caacggaaaa
     4081 gccattaaaa cttggtatgt tggtcatgcc accaaagacc acgatgccag ttacatgtta
     4141 cttgaaactc cttctgaagg aaaatccagt ctgccttatg tcacttacat tcccggattt
     4201 gccggagaat tatcgagtcg ctatttctgc gtggaagaag aatggcgttt tacgggaata
     4261 tttaattatg atccttcgga gattatagct gtttctattc gcaataacga ttatccggaa
     4321 gagagttttg aagtgaaagt gctcgacaaa aacctttttg ccttgcatga tcaattcggt
     4381 aaaaaagtcg agcgctttga taccgccgga gtacgtcagt tggtcgtcaa ttttaaaaag
     4441 atccattttg agacgctgaa ccgtggaata ctaagtccgc ttcaggaaga ttcgcttcgc
     4501 aaggctaaac cttattacga gataagcgtc accgataaaa aaggggagaa aaaggaaata
     4561 aaggtcttcc ataagaaatt gcccccgcac aatatgaata acgaaccctt tgagcaggac
     4621 tatccatacg atccggaacg ggcttttgcg cagatacccg ggggtgagat tgtggtgatt
     4681 cagtttttcg tattcgacaa aattctgtgg cccatcgatg ccttccttcc ccgcaaggaa
     4741 tgaaaaccgc ttgatatttc aaggttttac gggtttttca cgttgttaac attgggttgt
     4801 ttttaatcct tgaaaagttc cctgttcgtg ggggaccatt ccatctatat ttgttgagct
     4861 aaaaacctca tcaatgaaat tcattgtctc cagtacggcc ctgctcaagc agcttcaatc
     4921 catcagcggt gttttaaatt ccagcaacac gcttcccatt ctcgataatt tcctattcga
     4981 gatcaataag aagcagctta ccgtttctgc ctcag
//
LOCUS       DAOMYG010000238         4989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5827_length_4989_cov_10.328739, whole genome shotgun sequence.
ACCESSION   DAOMYG010000238 DAOMYG010000000
VERSION     DAOMYG010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5827_length_4989_cov_10.328739"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PK610_08480"
     CDS             complement(<1..777)
                     /locus_tag="PK610_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HRJ36011.1"
                     /translation="MKTRVIIHGGFFSESSTSMETKIAKQNALREIAGKAYTYLQDHS
                     AAETVVYAVALLENDILFNAGTGSQIQSDGKIRMSASLHDGNTLKFSGVINVEHVQNP
                     IYIAEHLMQVDDRVLGGEGAIRYARENGFENYNPEIPQRRNEYEAKLAASRTSTVGCV
                     ALDAEGRLSAATSTGGKGFEIPGRISDSATTAGNFCNADCGVSCTGVGEDIVSVALAT
                     KIVTRVSDGLTLEKAFEKSFAELKQIDGFAGAIAIDKNGNF"
     gene            complement(774..1655)
                     /locus_tag="PK610_08485"
     CDS             complement(774..1655)
                     /locus_tag="PK610_08485"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HRJ36012.1"
                     /translation="MAPKGILIIIGGAVDKGSFTESNFDKNVANNLNFFETGILKRII
                     TESFNKEHSRIEIITTASKIPKEVGPEYAKAFQYLGVENVHTLDIDKREQANDPEVLQ
                     RIHDADVVMFTGGDQLRLTSILGGTQFHDLLRQKYMNEHFVIAGTSAGAAAASTNMIY
                     QGSSSEALMKGEVKITQGLGFIDNVVIDTHFVQRGRIGRLFQAVVSNPQILGIGLGED
                     TGLLIKHNNNMEAIGSGLVILVDGREIKDTNLTSVELGSPISIEHLVVHVMSMQTHYD
                     LKHRKLEIGTAVKASSK"
     gene            1864..4497
                     /gene="cphA"
                     /locus_tag="PK610_08490"
     CDS             1864..4497
                     /gene="cphA"
                     /locus_tag="PK610_08490"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRJ36013.1"
                     /translation="MKIMSVQALRGPNIWSVTRKKLIQMRLDLEELEDFPTNKIDGFG
                     DRLKALIPSMYEHRCSEGTEGGFFSRVERGTWMGHVIEHIALEIQTLAGMDTGFGRTR
                     ETKTRGIYNVVFSYVEEKVGLFAAESSVRIADALIKGIDYDIQADIRKMREIREDVRL
                     GPSTGSIVDEAVARDIPWIRLGTNSLIQLGYGVNQMRFQATITCKTSNIAVDIACDKE
                     DTKRMLRNAAIPVAAGDICVDEEDLDATIKRIGYPIVIKPLDGNHGKGASINVKTWEA
                     AVEGLAHAKKYSRRVIVERFITGFDFRVLVIDNKVVAAAQRIPAHVTGDGKHTISQLI
                     EIVNKDPRRGYGHENVLTEIAVDRDTEELLLKNNQTLDSVPAKGEIVYLKSTANLSTG
                     GTSVDVTDMMHPENIFLAERISRVIGLDICGIDIMASNLTEPLKENGGVILEVNAAPG
                     FRMHLAPSEGLPRNVAAPVIDMLYPPGKPSRIPIIAVTGTNGKTTTTRLIAHMVKNMG
                     YKVGFTTSDGIYVQNHMLEKGDTTGPVSAEFILKDPTVEFAVLETARGGILRAGLGFG
                     RCDIGVITNIQEDHLGLNDIDTLDDLARVKSVVVKSVKKDGWAVLNAENTHCVKIAEE
                     LSCNIAYFAMDENHPVLREHCKKGGTAAVFENGFVTIKKGDWKIRVQKVTHIPLTMGG
                     RARFMIENVLAATLAGFLYGFKTEEMKLSLETFIPSAALTPGRMNVFNFRNFKVMIDF
                     AHNKAGYLAIEDFLSTVNSGHKVGIIAGVGDRRDDDIRDCGEIAARMFDHIIIRQEKH
                     LRGRTEEEIIDLILEGIKRVKKDVSYEIIPKEIEAIKHAITSAGEGTYITALSDVVTN
                     AIEIVQEYLDKETEEIENVNT"
     gene            4551..>4989
                     /locus_tag="PK610_08495"
     CDS             4551..>4989
                     /locus_tag="PK610_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRJ36014.1"
                     /translation="MRLGLLLLFFLFGQQVFSQDTLRTKGYYVIWDTLKMKERKDWTE
                     HFTSQGAADGTLVIYDQQKGKYQVYNRQRMVDRFIPASTFKIYNSLIALETGAVKSEK
                     ETFKWDGFDRGNGNWNRDQDMAFRCVTPHTRRLKRSPGTDVNPA"
BASE COUNT         1361 a         1051 c         1156 g         1421 t
ORIGIN      
        1 gaaatttccg tttttatcaa tggcaatggc tcctgcaaaa ccatcgattt gttttagttc
       61 agcaaacgat ttttcaaatg ctttttccaa tgttaatcca tcgcttaccc gtgtaacaat
      121 ttttgttgcc agtgccacgc tcacaatgtc ttctcccaca ccggtacacg aaacaccgca
      181 atctgcattg cagaaatttc ctgctgtagt ggccgaatca gaaattcttc ccgggatctc
      241 aaatcctttt cctccggttg aagtggctgc agaaagtcgt ccttctgcat ccaatgcaac
      301 acatcctaca gttgaagtcc tgcttgctgc cagctttgct tcgtattcgt tgcgacgctg
      361 ggggatttcg gggttgtagt tttcaaatcc attctcacgc gcgtatcgaa tcgctccttc
      421 tcctcccaat acacggtcgt ccacctgcat aagatgttcg gcaatataaa tggggttttg
      481 tacgtgctcc acattaataa ctcccgaaaa tttcagcgtg ttgccgtcgt gaagtgatgc
      541 actcattctt atttttccgt cggactgaat ctgtgatccc gttcctgcat tgaacaaaat
      601 atcgttctcc agtaatgcaa ctgcatacac tactgtttct gcggcactgt ggtcttgcag
      661 ataggtatag gcttttcctg caatttcgcg gagtgcattt tgttttgcaa tttttgtttc
      721 catactggtc gaggattcac tgaagaatcc tccgtggatg atcactcgcg ttttcatttt
      781 gaacttgctt ttacggctgt cccaatttca agtttgcggt gtttgaggtc gtaatgggtt
      841 tgcatgctca ttacgtgaac aaccaaatgt tctatcgata tcggacttcc caattcaaca
      901 gaggtgagat tagtgtcctt aatttctctt ccgtcaacca gaattaccaa tccagatccg
      961 atggcttcca tgttgttgtt gtgcttgatc aaaagtccgg tgtcttcccc gagtccgatt
     1021 ccaagaattt gcggattgct taccacagcc tggaacaatc gaccaattcg tccgcgctga
     1081 acaaaatggg tgtcgatcac aacattgtcg atgaagccca gtccttgtgt gatcttcact
     1141 tcacctttca tcagtgcttc actgcttgat ccctgataga tcatattggt agaagctgca
     1201 gcagcacctg cagatgttcc tgcaataaca aaatgttcgt tcatgtattt ctgccgtaga
     1261 agatcatgaa attgagtacc acccagaata gaagtgagtc gtagctgatc accgccggta
     1321 aacattacca catcggcatc gtgaatgcgc tgaaggactt ccggatcatt ggcctgttcg
     1381 cgtttatcga tatcgagtgt atgaacattt tctactccca gatactgaaa tgcttttgcg
     1441 tattccggtc cgacttcttt cggtattttc gaggcggtag ttatgatctc aatacgtgaa
     1501 tgttctttat tgaacgattc agtaatgatc cgcttcagaa ttccggtttc aaaaaaatta
     1561 agattgttgg ctacgttttt atcgaaatta ctttctgtaa aactaccctt atctactgcc
     1621 cccccaatga tgattaaaat acccttcggt gccattttct ttgagtttga gatagcaaaa
     1681 tagggataat atcgaccact tcacctcatc ggtacggggt tctttcaggt ggtctttgtt
     1741 gatttcctgc ttagcgatga aatcctcact ttcaggcgaa taatgatgga tttggtctaa
     1801 ggcggtgggt tggattttca ggtctcggat tttactgcta cattactttt aaatactatt
     1861 gccatgaaga taatgagcgt tcaggccctg aggggaccca atatctggag cgtgaccaga
     1921 aaaaagctga ttcagatgcg tctggatctc gaagagcttg aagattttcc caccaataaa
     1981 attgacggat tcggtgatcg tctcaaagca ttaattccga gtatgtacga acaccgttgt
     2041 tcggaaggaa cggaaggcgg attcttttcg cgtgtggaaa gaggaacatg gatgggtcac
     2101 gtgatcgaac atatcgctct cgaaattcaa acgctcgcag gcatggacac cggttttggt
     2161 cgcacacgcg aaacaaaaac ccgcggtatc tacaatgtag tattcagtta cgttgaagaa
     2221 aaagtaggat tgtttgcagc tgaatcttcg gtacgaattg ccgatgcatt gatcaaagga
     2281 attgactacg atatacaagc cgatatccga aaaatgcgtg agatccgtga ggatgtgcgt
     2341 ctaggtccaa gtaccggctc tattgttgat gaagctgtag caagagatat tccatggatt
     2401 cgcctgggaa caaattcgct tattcagttg ggttatggtg taaaccaaat gcgttttcag
     2461 gcgaccatta cctgcaaaac ttcgaatatt gcggtggata ttgcctgcga taaggaagat
     2521 actaaacgaa tgctgcgcaa tgctgctatt cctgttgctg caggagatat ttgtgtggac
     2581 gaagaagatc tggatgctac cattaaacga ataggttatc ctattgtaat taaaccgctg
     2641 gatggcaatc atggcaaagg agcctccatc aatgtaaaaa catgggaagc ggcagttgaa
     2701 ggattagcac atgccaagaa atattcgcgc agagtaattg ttgaacgttt tattaccgga
     2761 ttcgattttc gagtactggt aatcgacaat aaagtagtag cagcagctca gcgtattcct
     2821 gcccatgtta cgggtgatgg aaagcacacc atttcgcagc tgattgaaat tgtaaacaaa
     2881 gatccgcgtc gaggttacgg acatgaaaat gtgcttaccg aaattgctgt ggatcgtgat
     2941 acagaagaat tgttgctcaa gaacaatcaa accttggaca gtgttcctgc gaaaggtgaa
     3001 attgtatact tgaagtcgac tgcgaacctc agtacaggcg gaacatctgt agatgtgaca
     3061 gacatgatgc atcctgaaaa tatttttctt gcagagcgta tttcgagagt gatcggactt
     3121 gatatctgcg gaattgatat tatggcttcc aatttaactg agcctcttaa ggaaaacggt
     3181 ggagttattc tggaagtgaa tgccgcaccc ggattccgca tgcaccttgc accatcagaa
     3241 gggttaccac gcaacgttgc agcgcctgtc attgatatgc tctatcctcc cggaaaacca
     3301 tcacgtattc cgattatcgc ggttaccggt accaacggaa aaaccacaac aacgcgactg
     3361 attgcgcaca tggttaagaa catggggtac aaagttggat ttacaacttc agacgggatt
     3421 tatgtgcaga accacatgct tgaaaagggc gatactaccg gtcccgtttc tgcggagttt
     3481 attttgaaag atcctacagt tgaatttgcg gtactcgaaa cagcacgcgg aggaattctt
     3541 cgtgcaggat taggttttgg tcgttgcgat atcggtgtca tcacgaacat acaggaagat
     3601 catttgggac tgaatgatat tgataccctc gatgatcttg cgcgtgtgaa aagtgtggtt
     3661 gtcaaaagcg taaagaaaga cggttgggcg gtgctcaatg cagagaacac acattgcgtg
     3721 aaaattgcgg aggagctcag ctgcaacatc gcttattttg caatggatga aaaccatcct
     3781 gtacttaggg agcattgtaa aaaaggtgga accgcagccg tttttgaaaa tggttttgta
     3841 acgataaaaa aaggcgactg gaaaattcgc gtgcaaaagg ttacgcatat tccactcaca
     3901 atgggtggac gggcgcgttt catgattgaa aacgttttag cagcaacatt agccggattc
     3961 ctctacggat ttaaaaccga agagatgaaa ctttctcttg aaacatttat tccctccgca
     4021 gcccttacac cgggaagaat gaatgttttc aatttcagga attttaaggt gatgatcgat
     4081 tttgctcata acaaggcagg ttatcttgcc attgaagatt tcctgtcgac cgttaattcc
     4141 ggtcacaaag tgggtatcat tgccggtgtt ggtgatcgac gcgacgacga tatccgcgat
     4201 tgtggtgaaa ttgcagcacg tatgttcgat cacatcatca tccgtcagga aaaacatctg
     4261 cgtggaagaa ccgaagaaga gatcattgat ttgatcctcg aaggcatcaa gcgggtgaaa
     4321 aaggatgttt cgtatgagat cattcccaag gagatcgaag ccatcaagca tgccattaca
     4381 agcgccggag aaggcacgta tattaccgcg ctgagtgatg ttgtaaccaa tgcaatcgaa
     4441 attgtgcagg aatatctcga taaggaaacg gaagaaatag agaacgtgaa cacctgatga
     4501 tgaaacctgt ttttgtttgt tatttctccc taatccaata acttcgcatc atgcgtttag
     4561 gtttattact tctttttttc ctgttcggtc aacaggtttt ttctcaggat acactgcgga
     4621 ccaaaggata ttatgtcatt tgggatacgc tcaaaatgaa agagcgtaag gattggactg
     4681 agcatttcac ttcgcaggga gctgcagacg gaacacttgt gatttatgat cagcagaagg
     4741 gaaaatacca ggtctataac cgtcagcgta tggtcgaccg ttttattcct gcctcaacat
     4801 ttaaaattta caattcgctg attgcattgg aaacaggtgc ggtaaaaagt gaaaaggaaa
     4861 cgtttaagtg ggatggattt gatcgtggca acggcaactg gaacagggat caggatatgg
     4921 cttttcggtg cgtaacgcct cacacacgga ggctaaaacg ctctccagga actgatgtaa
     4981 atcccgcgt
//
LOCUS       DAOMYG010000239         4983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5837_length_4983_cov_7.808644, whole genome shotgun sequence.
ACCESSION   DAOMYG010000239 DAOMYG010000000
VERSION     DAOMYG010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5837_length_4983_cov_7.808644"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PK610_08500"
     CDS             <1..408
                     /locus_tag="PK610_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRJ36015.1"
                     /translation="DKALAGNRIGDISAAIQHHAEKNGFGVVRELVGHGLGTELHEDP
                     QVPNYGRKGYGPKIYDGLVIAIEPMINMGTRNIKQSSDGWTITTADNKPSAHFEHDVA
                     VVNGRPDILSTFSYVEEALRKKGAWMPSLNVSV"
     gene            complement(415..2340)
                     /locus_tag="PK610_08505"
     CDS             complement(415..2340)
                     /locus_tag="PK610_08505"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ36016.1"
                     /translation="MTKPFIFQSVFVVLLMFEVRAESPRKSYDVLLNLVEVSWKYTQT
                     NPDTAIILSLQGIEISKRQGHLQLESRFLSNMAEAHRTKGNYSMAIELLLESISIYEK
                     ISDAPGQASTLNRMGAVYLEMGQIDTAIDYFMRALVKYESLRDLVGSSRSLNNLGACY
                     FYEGDFRKAIDFFSRSLDVELLNNNKNGVCDSYVNIGSALFGLDEFSQAESYFMKALK
                     LAEELKDSSTIGSLYNNMATLKMEQGDYKNAISYFHNALDLAYKSDNNENIRQFLLNI
                     ANAYEKSGEYRLAFENLRVYIGFRDSTLNEQNQHAIAEMTTKYKSEQKEKENQLLKKN
                     GQIKDLELDKQKAILYGVSAALLLMAVLGLLIWRQYRQKIKANTILQEQRDTIAAQKK
                     DLTDSIVYARHLQDAILPETNRLSHFFTEHFLIYLPKDIVSGDFFWLMETEEDLYLAV
                     ADCTGHGVPGAMVSMVCHNALNRSVSEFGIKETNRILDKCAELIEQTFSANRQSMKDG
                     MDISLMRLTKEPGKIQWSGANNPFWIIRKGEVEVESLKPNPQPIGSFFGRSPFHAHEC
                     LLNTGDCIYLFTDGIVDQFGGPNGKKLKAVGLKKILLQFHHLPHEEQRLQVMSAFENW
                     KGDVEQIDDVCLVGIRV"
     gene            2434..2757
                     /locus_tag="PK610_08510"
     CDS             2434..2757
                     /locus_tag="PK610_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36017.1"
                     /translation="MESTGIVIEISEDRKSALIVPDKGGDPISYKGKELKPNIDVNEG
                     VSFVFLDGKATDVSLITTVKTEGEILREERELIRQLTVLINQRMGGTKRPPGGCVVKI
                     KTRDF"
     gene            complement(2826..>4983)
                     /locus_tag="PK610_08515"
     CDS             complement(2826..>4983)
                     /locus_tag="PK610_08515"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRJ36018.1"
                     /translation="FTYRDGRHGFPCLSKGGNFKQVPLSIDCYGMGTFILGKDTLLLV
                     SGVDGVYEVDRDRKPLRIFEGFPWTVKQSVTYANRIIIAEEGSLASMVRMDGHWEAEG
                     RVEGIEADVTNFVEETNGDLWLGTTDMGVIRTTTKIFKDKKTEILFYDESFGLTDGPV
                     VITQNKAGELYFGTDNGVCRFDEKKQRFFQDEKYKVPTKTGKSGIHRLSFDAKGQLWV
                     AVFYDEGKSYDIMYYRNNSWYKTPFLRYNSEIFHCIYHEPKGITWLGSAAGLLRYDQE
                     FLKDYQTPYHVGIRRVLSGNNPIFEGAFFDRDSVLLDLQPEDADVTLTFSQNNLTFEF
                     SALTYFDELGTEYSYMLEGQTEDWSKWSTNPRAIFTNIPEGSYTFKVKARNIYDIESP
                     VSEFRFVILPPWYRTLWAYIGYAILFIAFVWGAISVSTRSLRNIIEERTAEITLQKEE
                     IEKQKEIVEEKNKDILDSIKYAKRIQDAILPEEETMREVMGKDLFVLFQPKDIVSGDF
                     YWMRVKGPKVLFAAVDCTGHGVPGAFVSIVGNNGLNRAVNEFNLTQPAAILDNLTASV
                     EDSFRQQGHSDVRDGMDISLCSLEMTGFNEALLEWAGANNPLWIIRKDNPTAVEEYKA
                     DKQPIGKFENRKPFTNQQFELKKGDTIYVFTDGYADQFGGPAGKKFKYSQMKEFILSI
                     QEMDMIAQRNLLLERFAQWKSELEQIDDVCVIGVRI"
BASE COUNT         1507 a         1113 c          930 g         1433 t
ORIGIN      
        1 gacaaagcac tggccggtaa cagaattggc gatatcagcg cagccataca gcaccatgcc
       61 gaaaaaaatg gatttggagt agtacgggag ctggtaggac atggattggg cacagaactg
      121 catgaagatc cacaagtacc gaattacggc cgtaaaggct acggaccaaa gatctatgac
      181 ggattggtaa ttgcgatcga gcccatgatc aatatgggaa cccgaaacat caaacaatca
      241 tccgacggct ggacgattac cactgcagat aataaaccat cggcacattt tgagcacgat
      301 gtggccgtag ttaatggaag accggatata ctatcaacat ttagttatgt ggaagaagca
      361 ctccgcaaaa aaggagcctg gatgccctcg ttgaatgttt ctgtttaatc gtatttacac
      421 tctgatacca accagacaaa catcatcgat ttgttcaaca tcacctttcc agttttcgaa
      481 agcagacatt acctgaagcc gttgttcctc atgcgggaga tgatggaatt gtagcagtat
      541 tttttttaac ccgacagctt ttaatttttt accgttcggt ccaccaaatt ggtccacaat
      601 tccatcggta aacaaataga tacaatcgcc tgtgttaagt aaacattcgt gcgcatgaaa
      661 gggcgaccgt ccaaagaaag atccgatcgg ttgagggttt ggttttaaac tctccacctc
      721 tacttcacct ttccgaataa tccaaaacgg attatttgca cctgaccact gaattttacc
      781 gggctctttt gttagtcgca taagggaaat atccattcca tctttcatgc tttgacgatt
      841 ggcagaaaac gtttgctcaa tcaattcggc acatttatcg agaatcctat ttgtttcttt
      901 tattccgaat tcactcacac tacgattcaa tgcattgtgg caaaccatgc tcaccatggc
      961 accgggaaca ccatgtccgg tacaatcggc aactgcgaga taaagatctt cctcagtctc
     1021 cattagccag aaaaaatcac cgctaacaat atcctttgga agataaatca gaaaatgctc
     1081 ggtgaagaaa tgggaaagcc gattggtttc aggtaagatg gcatcctgca aatgccgggc
     1141 ataaacgata ctatctgtaa gatccttttt ttgtgccgca atggtatcac gttgttcctg
     1201 caaaatggta tttgctttta ttttctgacg gtattgcctc caaatgagca acccaagcac
     1261 tgccatcaac aacagagccg ctgacacacc atagagaata gccttctgtt tatccagttc
     1321 aagatctttg atctgaccat tcttctttaa aagttgattc tctttttctt tttgttcgga
     1381 cttgtacttg gtagtcattt cagcaatggc atgctgattt tgttcattca aagtagaatc
     1441 gcgaaaaccg atataaaccc gaagattttc aaacgccaaa cggtattccc cactcttttc
     1501 ataagcgttt gcaatgttca gcaaaaactg acggatattt tcgttgttat cggatttgta
     1561 ggcaagatca agtgcattat ggaaatatga aattgcattt ttataatccc cctgctccat
     1621 tttaagcgtc gccatattat tgtaaagact tccaatggtg gaagaatctt ttaactcctc
     1681 cgccaacttt aacgctttca taaaataact ctcggcctga gaaaattcat caagtccaaa
     1741 caatgcagat ccgatattta catagctgtc acaaaccccg tttttattgt tgttgaggag
     1801 ttcaacatca agagatcttg aaaaaaagtc gattgctttc cggaaatcac cttcataaaa
     1861 ataacaagca ccaagattat tcagcgaacg tgaggagcca accaagtccc ttaaactttc
     1921 atatttcacc agcgcacgca taaaataatc gattgcggta tctatttgcc ccatctcaag
     1981 gtagaccgct cccatcctat tcaaagtaga cgcttgtccg ggtgcatcgc ttattttctc
     2041 gtagatgcta atcgattcca gcagcaactc aatggccatt gaataatttc ctttggtacg
     2101 atgagcctcg gccatattac tgaggaaacg tgattccaat tgcaaatgcc cttgtcgttt
     2161 cgaaatttca atcccctgta aggataaaat aatcgctgta tccggattgg tttgggtata
     2221 cttccaggat acttctacca aattcaacaa aacatcataa ctcttccggg gactttcagc
     2281 acggacctca aacatgagca acacgacaaa aactgactga aatatgaagg gttttgtcat
     2341 gccggatgga aaaattcgat gtctgtagct ccaaaacata tcattaaata tagtttcttt
     2401 tattaaattg agccaactaa aaaaatcaga actatggaat ctacaggaat tgtaattgaa
     2461 attagtgagg atagaaaatc tgcactaatt gtacccgaca aaggcggaga ccctatctct
     2521 tataagggaa aagaattaaa gccaaacatt gatgttaacg aaggcgtttc atttgtgttt
     2581 cttgatggta aagcaactga tgtaagccta attacaacag taaaaacaga gggagaaatt
     2641 cttcgtgagg aaagggaact gatcaggcaa ctaaccgttt taattaacca acgtatgggc
     2701 ggaacaaaaa gacctcccgg aggttgtgtt gtaaagatta aaacaagaga cttttaatcc
     2761 aaagtcaaac caaaatcccc gcaaagcggg gattttttat tgatggaaat aaataatcga
     2821 aacaattaaa tccggactcc aattacacaa acgtcatcga tctgttcaag ctcactcttc
     2881 cattgggcaa atcgctcaag taataaatta cgttgtgcaa tcatatccat ttcctgaatg
     2941 gaaaggataa actccttcat ttgcgaatac ttgaatttct tacctgcagg tccgccaaat
     3001 tgatcggcat aaccatcagt aaatacataa atcgtatctc cttttttcag ttcgaactgt
     3061 tggttggtaa acggtttacg gttttcaaac tttccaatag gctgtttgtc ggccttgtat
     3121 tcctccactg cagtaggatt atctttccta atgatccaaa gcgggttgtt tgcaccggcc
     3181 cactccagca gcgcttcatt aaagccggtc atttcgagac tgcacaatga aatatccatt
     3241 ccatcgcgca catccgaatg accttgctga cggaaactat cttctacaga ggcggtgagg
     3301 ttatcaagaa ttgctgcagg ctgggttagg ttaaactcat ttacagcacg gttcaatcca
     3361 ttgtttccga caatagaaac aaatgcaccg ggtacaccat gacccgtaca gtcaaccgct
     3421 gcaaaaagaa cttttggtcc tttaacgcgc atccagtaaa aatctcccga tacaatatct
     3481 tttggttgga aaagaacaaa cagatctttg cccattactt cacgcatcgt ttcttcttcc
     3541 ggaagaatag catcctgaat tcgttttgcg tacttgatac tatcaagaat atccttattc
     3601 ttttcttcta caatttcctt ttgcttttca atttcttcct tctgaagtgt gatctctgct
     3661 gttctttctt caatgatatt ccgcaggctt cgggtagaaa cagaaattgc tccccataca
     3721 aatgcaataa ataaaatcgc ataaccgata taggcccaaa gtgtacggta ccaaggtggt
     3781 aaaatcacaa agcggaattc ggaaacagga ctttcgatat cgtaaatatt tcgggctttt
     3841 accttaaacg tgtaagaacc ttcggggata ttggtaaaaa ttgcacgcgg gtttgtcgac
     3901 catttgctcc aatcttctgt ttgcccctca agcatgtagg aatattctgt gcccaactca
     3961 tcaaaatagg tcaatgcaga aaattcaaac gtcaggttat tttgcgaaaa ggtcagcgta
     4021 acatcagcat cttcgggttg taaatcgagt agaacagaat cacggtcaaa aaacgctcct
     4081 tcaaaaattg gattatttcc ggataacacc ctgcggatcc ctacatgata cggtgtttga
     4141 taatccttga gaaattcctg atcataacgc aataatcctg ctgccgaacc aagccaggta
     4201 atgccttttg gttcatggta aatacaatga aaaatttcgc tgttatagcg taaaaatggc
     4261 gttttgtacc atgaattatt tcggtaatac atgatatcgt atgactttcc ttcgtcataa
     4321 aaaacagcta cccacagttg accctttgca tcaaacgaca aacggtggat tcccgattta
     4381 ccggtttttg tgggcacttt gtatttctca tcctggaaga aacgctgttt tttttcatcg
     4441 aaacgacaaa caccattgtc tgttccgaaa tacaattcac ctgctttatt ttgggtgatt
     4501 acaaccggcc catctgttaa gccaaacgat tcatcataaa agagaatttc cgttttcttg
     4561 tccttaaata tttttgttgt ggtacgaata actcccatat ctgttgtacc caaccagaga
     4621 tcaccattgg tttcctctac aaaattggtt acatcagctt caattccttc taccctgcct
     4681 tctgcttccc agtgaccatc cattcgaacc attgaagcaa gcgatccttc ttctgcaata
     4741 ataattcgat tggcataggt aactgattgc tttaccgtcc atggaaaacc ttcaaaaatt
     4801 cgcaagggtt tgcgatcgcg atcaacttca taaaccccat ctacaccaga aaccagcaat
     4861 aaggtatcct tgccgaggat aaatgttccc atgccgtaac aatcgatgga taaggggacc
     4921 tgtttaaaat tccctccctt ggataggcac ggaaatccat gtctcccatc ccggtaagta
     4981 aaa
//
LOCUS       DAOMYG010000240         4978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5848_length_4978_cov_9.601463, whole genome shotgun sequence.
ACCESSION   DAOMYG010000240 DAOMYG010000000
VERSION     DAOMYG010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5848_length_4978_cov_9.601463"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..636)
                     /gene="ruvC"
                     /locus_tag="PK610_08520"
     CDS             complement(52..636)
                     /gene="ruvC"
                     /locus_tag="PK610_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002976203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRJ36019.1"
                     /translation="MLQSQNQDTPAPSERIILGIDPGTNIMGYGVLLVKSKKMQLLDM
                     GVFRLEKLETQQDKLQRILEETLRVIDTFHPDEMAVEAPFFGKNIQSMLKLGRAQGVA
                     FAAALHRKIPVVEYSPSKIKQSITGKGSASKEQVAAMLSQLLQTEAKPKFLDATDALA
                     VAVCHYFQRNSVSGGKSYSGWEAFVKQNPNRKKS"
     gene            699..1799
                     /locus_tag="PK610_08525"
     CDS             699..1799
                     /locus_tag="PK610_08525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ36020.1"
                     /translation="MSTYIFSVLLIGVTALYCLTIIWFSMGVVRLVSLKKPKQTFTGK
                     VSVIVPFRNEHMRMKPLLESLSKQQLQNVEFIFVDDHSQDSTAEVIHSFAFSNYCILP
                     SNGSGKKSAIRTAVAKAKGEVLLFTDADCIVPSCWIEEMTLPFSAEGADLVAGPVSIK
                     GRGWLTGIQYWDQIALVASSAGSGAMGMPFMCSGANLAVNAACYPCDSILGDERSSGD
                     DVFLLHHLHREKKNIRFTWNKDACVLTLPESSWRAFFKQRMRWAGKASSYSNGMAVFV
                     SLLVLFCSASLLFGGLLSAIHPSLLSTLLICLGLKAAFDFLFLLLSERGLGHSGRLHY
                     FLPAFFFHVLYILPVAVLSQLVRAEWKNRKIN"
     gene            1803..3065
                     /locus_tag="PK610_08530"
     CDS             1803..3065
                     /locus_tag="PK610_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ36021.1"
                     /translation="MARKKTVQTSKEVSRSLSAMAWKRLKRNYPAMFGFFVILFACMI
                     AILGALIRPDKTTNAANAIPAVNTKPPGFEVKYLLVQKNDTIHPTTFFDKMLFGGEEN
                     KFRYVPITEFEFRGAEISVKEYVGEEENSQFRQISIPYLLADVLYPLHMNNRYSEDGQ
                     GNVSFYDIHNNKITRSIIEMQNEVESRCIIDQTYWLGTDRNGRDMLSRLMAGTIVSLS
                     VGLISVLISLIIGISLGAIAGYFRGWVDDVVMWLINVVWSIPTLLLVIAVTIALGKGF
                     WQVFVAVGLTMWVEVARIVRGQVLSIREKEFVEAGRALGFRAPRIILRHVLPNVMGPV
                     IVISAANFASAILIESGLSFLGVGVQPPMPSWGSMIAQHKDYITTEMAYLAVLPGIAT
                     VVMVLAFMLVGNGLRDALDTKAVDDVRS"
     gene            complement(3119..3481)
                     /locus_tag="PK610_08535"
     CDS             complement(3119..3481)
                     /locus_tag="PK610_08535"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HRJ36022.1"
                     /translation="MYTLKGQIKLVQPTQNISEKFRKREIVVVENSSQYPQFITVQFT
                     NDKCDLLDSFKPGEEVEIGFFLRGREWNDPKTGSTRYFNSIDGFKIMRTTNPIGAESP
                     DFPPANITGGDDIDDLPF"
     gene            complement(3509..4402)
                     /locus_tag="PK610_08540"
     CDS             complement(3509..4402)
                     /locus_tag="PK610_08540"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRJ36023.1"
                     /translation="MFRVDPKETPIPKLHGYLLGAVGPRPIAFASTIDKTGKANLAPF
                     SFFNVFSANPPILIFSPARSGRTNTTKHTYDNVKEVAEVVINIVNHDMVHQMSLASSE
                     YAKGVDEFIKSGFTPIASEKVKPFRVKESPVQLECKVNQVIELGNQGGAGNLIICEVV
                     LIHINETILDAKGVIDQQKIDLVARMGGNWYCRAHGDALFEIEKPLTTLGIGVDAIPG
                     DIRNSRFLSGNHLGQLGNVESLPDETDVNEYKLLELSDLFIAHEKDAAKLENELHKHA
                     ALLLDQNKVKEAWMTLLSFNN"
     gene            4765..4846
                     /locus_tag="PK610_08545"
     tRNA            4765..4846
                     /locus_tag="PK610_08545"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4799..4801,aa:Leu,seq:tag)
BASE COUNT         1264 a          976 c         1163 g         1575 t
ORIGIN      
        1 attactcagg aagagcttgc tgagattgcg aatttgatcc ggaattctta atcaggattt
       61 tttgcggttg ggattttgtt ttacaaatgc ctcccatccc gaatacgatt tccctccact
      121 tactgaatta cgctggaagt aatgacaaac agcaaccgcc agcgcatctg ttgcatccag
      181 gaatttaggt ttagcctcag tttgaagcag ctgtgagagc atagccgcaa cttgctcctt
      241 tgatgcactt ccttttcctg taatggattg tttgatctta cttggagaat attccaccac
      301 aggaattttg cggtgtaatg cagctgcaaa tgccactcct tgcgctctgc ccagtttcag
      361 catggattgt atgttctttc caaagaaagg agcctcaact gccatttcat cgggatgaaa
      421 ggtgtcaatt acacgaagcg tttcttcgag aatgcgctgt agtttgtcct gttgggtttc
      481 cagtttttcc agacggaaaa cacccatatc cagcaattgc atctttttgg atttcaccag
      541 caacacaccg taacccatga tgtttgttcc agggtcaatt ccaagtatga tgcgctcaga
      601 gggcgctggt gtatcctgat tttgtgattg cagcatttca tcaaagataa ttaacccatt
      661 tccgcgatat tgcgttctga aaaggaattt tccacattgt gtcgacttac attttttctg
      721 ttctgctaat tggagtaacc gcactttatt gcttaaccat catatggttt tcaatgggtg
      781 tagtacgatt ggtttcactt aaaaaaccaa aacaaacttt tacaggtaag gtttccgtta
      841 ttgttccgtt tcgcaatgag catatgcgga tgaagccctt attggagagt ttgtcaaaac
      901 aacaactgca gaatgtggag ttcattttcg tcgacgatca ttcgcaggat agcaccgcag
      961 aagtgattca ttcgtttgcc ttttccaact attgcattct tccttcaaac ggatccggga
     1021 agaaaagcgc aataagaacc gccgttgcca aggcaaaagg agaagtattg ttatttactg
     1081 atgccgattg cattgttcct tcctgctgga ttgaggaaat gaccctgcca ttttccgctg
     1141 aaggcgcaga tcttgttgca ggacctgtgt caattaaagg tagaggatgg ttaacaggga
     1201 tacagtattg ggatcagatt gccctggttg cttcttcagc cggatccgga gcaatgggta
     1261 tgccgtttat gtgcagcggg gcaaatcttg ctgttaatgc agcatgttat ccatgtgatt
     1321 ctattttggg ggatgaacgg agtagtggag acgatgtttt cctattgcat catcttcata
     1381 gggagaaaaa gaatattcga tttacctgga ataaagatgc ttgcgttttg actttgcctg
     1441 aatcttcttg gagggccttc tttaaacagc gaatgcgttg ggcaggcaaa gccagcagct
     1501 attctaacgg aatggctgtt tttgtatcct tattggtgct cttttgctcg gcatcactgt
     1561 tgtttggtgg gctgctttca gccattcatc cttcgctttt gtccactctt ctcatttgcc
     1621 ttggactcaa ggcagcattt gactttttgt ttttactttt gagcgaaagg ggtttgggac
     1681 atagtggccg acttcattat tttctacccg ctttcttttt tcacgtcctg tatattcttc
     1741 cggtggcagt gttatcgcaa ctggttcgtg cagagtggaa aaacagaaag attaattaag
     1801 caatggcaag aaaaaaaaca gttcaaacca gcaaggaagt ttcgcgatcc ctctcggcaa
     1861 tggcctggaa gcgattgaag cgaaattatc cggcaatgtt tgggtttttc gtgattctgt
     1921 ttgcttgtat gattgcaatt ctgggcgcgc tgattcggcc ggataaaacc acaaatgctg
     1981 ccaatgctat tcctgcagta aataccaagc cccccggatt cgaggtcaaa tatttactcg
     2041 tgcaaaaaaa tgacaccatt catccaacaa cgtttttcga taagatgttg tttggtgggg
     2101 aagaaaataa attccgttat gtcccaatta ccgaatttga atttcgcggt gcggaaatca
     2161 gcgtaaagga atatgttggc gaagaagaga attcacagtt cagacaaatc agtattccct
     2221 acttgctggc agatgtattg tatccactgc acatgaataa ccgttattcg gaagatggtc
     2281 agggtaacgt ttcattttat gatattcaca acaataaaat aacgcgttcc attatcgaaa
     2341 tgcaaaatga agttgaatcg cgttgcatta tcgatcaaac ctattggctg gggacagaca
     2401 ggaacggtag ggatatgctc agtcgactta tggctggtac gattgtatcc ttgtctgtgg
     2461 gacttatttc tgttttaatt tcattgatca ttggtatttc gctgggtgca attgccggtt
     2521 acttcagagg ttgggtagat gatgtggtaa tgtggttgat caatgtggtt tggtccatcc
     2581 ctactttgtt attggtaatt gcggttacta tagcactggg aaaaggattc tggcaagtgt
     2641 ttgttgctgt tgggctcaca atgtgggttg aagttgcaag aatagttcgt ggtcaggtgc
     2701 tttctattcg ggagaaggag tttgttgaag ccggacgtgc tttaggattt cgtgcacccc
     2761 gtattatttt gcgccatgta ttgccaaatg taatgggacc ggtaatcgta atttctgctg
     2821 caaattttgc gtctgctatt ttaattgaaa gcggtctttc tttccttggt gttggtgtac
     2881 aacctccaat gccttcatgg ggctcaatga ttgctcagca taaggattat attacgacag
     2941 aaatggctta cctggcagtt cttccgggaa ttgcaacggt agtgatggtg cttgcgttca
     3001 tgctggttgg aaacggcttg cgtgatgcgc tggatacaaa ggcagtggat gatgtcaggt
     3061 cctaaatgaa aaacataaaa aaagcccgga ttttccgggc ttttttgttt ctgataaatc
     3121 agaatggaag gtcatcgata tcatctccgc ctgtgatgtt tgccggaggg aagtcaggtg
     3181 attcagcacc aataggattt gtagtgcgca tgattttgaa accgtcgatt gaattgaaat
     3241 aacgagtgct tcctgtttta ggatcgttcc attctcttcc acgaaggaag aagccaattt
     3301 ctacttcttc accgggttta aatgaatcga gaagatcgca tttgtcgttg gtaaactgaa
     3361 cagtaatgaa ttgtggatac tgcgaagaat tttctacaac aacgatttcg cgtttacgaa
     3421 atttctcgct gatgttttgc gtcggttgaa cgagcttaat ttgtccttta agtgtataca
     3481 taacaaaaaa ttgatgagcg tattttggtt aattgttaaa gcttaatagt gtcatccatg
     3541 cttcttttac cttgttttga tcgagtagta aggcggcatg tttatgcaat tcgttttcga
     3601 gtttggcagc atctttttca tgagcaataa aaagatcgct gagttcaagt aatttgtatt
     3661 cgttcacatc tgtctcatca ggaagtgatt cgacgtttcc aagctgacca agatgatttc
     3721 cgcttaggaa acgggaattg cgaatatcac ctgggatagc gtcaactcct attcctaggg
     3781 tggtaagggg tttttcaatc tcaaaaagcg catcgccatg agctcggcag taccaattgc
     3841 ctcccatgcg ggctaccaga tcaatttttt gctgatcaat tacacctttt gcgtcgagaa
     3901 tggtttcgtt gatgtgtatt agaacaacct cacaaatgat caaattaccg gctcctccct
     3961 ggtttcccag ttcaataacc tggttcactt tacactccag ctgcactggg ctttccttaa
     4021 cccggaaggg tttaactttt tctgatgcta ttggagtaaa tcctgattta ataaactcat
     4081 caactccttt ggcgtattct gaactggcca gtgacatttg atgaaccatg tcgtgattca
     4141 cgatgttgat tacaacctca gcaacctctt ttacattatc ataagtatgc ttggtggtgt
     4201 tggttctgcc actgcgcgcc ggactaaaga tcagtattgg aggattagca ctgaagacat
     4261 taaagaaact aaaaggcgca aggttggctt tgcctgtttt gtcaatggtg cttgcaaagg
     4321 caattggacg aggtccaact gcacccagca gatagccgtg tagtttaggg ataggagttt
     4381 ctttggggtc gacgcgaaac atggtattgt gatgagatgt gcaaagttaa ccaaagggca
     4441 ggtaagaaaa ggcttaaaat atccacaagt taatgcttcg gttggagtag tgttaataag
     4501 cactttagga gaattggaat atttcagtat ttttgtgctc cgtttctgaa ggggatagga
     4561 tttccttcaa atcggtttga acctcgttct tttgtagtaa attttttatg cgggcgtggc
     4621 gaaattgccc tgacgcaagg ctcagacgag tcttcgccca ggtcaggatc cacgaagttc
     4681 taaacctcga caaatgtctc ggcaagaact ttcgggagta gacgcgattc gttttttata
     4741 gttcttttgt agtaaatttt ttatgcgggc gtggcgaaat tggtagacgc actagactta
     4801 ggatctagcg ccgcgaggca tgggggttcg agtccctccg cccgcacaac ctaaaaaaaa
     4861 gcgaatgatc agtggttcat tcgctttttt ttgctccata aaacagtttg tcgtcattca
     4921 ttttgggatt ttaaatcttt atcccaataa tgcatatgtc atcaacttgt tcgtggtc
//
LOCUS       DAOMYG010000241         4967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5863_length_4967_cov_10.763640, whole genome shotgun sequence.
ACCESSION   DAOMYG010000241 DAOMYG010000000
VERSION     DAOMYG010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5863_length_4967_cov_10.763640"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PK610_08550"
     CDS             complement(<1..308)
                     /locus_tag="PK610_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRJ36024.1"
                     /translation="MNFEYTEEQLAVRAAARDFAQNVLKPGVIDRDREMRFPKDEIRQ
                     LGELGFMGMMVDPKYGGGGMDTVSYALAMEEISKIDASVSVCMSVNNSLVCWGLEKFG
                     T"
     gene            380..1444
                     /locus_tag="PK610_08555"
     CDS             380..1444
                     /locus_tag="PK610_08555"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRJ36025.1"
                     /translation="MKSMHILGLMSGTSLDGLDMALCEFSHTDAYHYRIEATRFIPYN
                     KQWKKKLAEAMLLHSTDLLALDAEYGQWLGEQCVEFLKSHAGIPCDAIASHGHTVFHQ
                     PEKGFSLQIGSGDHIAAISGKLTVNDFRSGDIALGGQGAPLVPIGDLLLFGEYAMCLN
                     LGGIANISGTSEGKRRAFDISVANMALNHLAGKLGMDYDDKGNFSRKGTTLPALLKQL
                     NDIPFFQQDGPKSLGKEWFDKEFLPLIENSECPAEDCLSTVCEHIAFQLSNAMNRFNP
                     GKVLVTGGGAYNDYLIARIQAHTQNEIIVAEKELISFKEALLFAFLGWLRINECTNSL
                     ASVTGASRDACCGVIRMGKQ"
     gene            1552..2778
                     /locus_tag="PK610_08560"
     CDS             1552..2778
                     /locus_tag="PK610_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HRJ36026.1"
                     /translation="MKVKLKQFETKRPEIVFEWKDAETDAEGWVVINSLRGGAAGGGT
                     RMRKGLDKREVESLAKTMEVKFTVAGPPIGGAKSGINFDPADPRKKGVLERWYKAVAP
                     LLKNYYGTGGDLNVDEVHDVIPITNALGVLHPQEGVVNGHYGANETDKKKSISNLQSG
                     VSKVLNDVRYSPDVTRKYVVADMITGYGVAEAVKHFYAIYGGDAKGKKVIVQGWGNVG
                     SAAAYYLAQQGAIVVAIIDRDGGLVNENGFSFQEIRKLFIEKDGNKLKADNVIPFAEL
                     NKTVWDVKADIFLPCAASRLITEDQLNRMINAGVNVVSAGANVPFADPEIFFGPIGEK
                     ADEKLSLLPDFIANCGMARVFAYLMQPTATITDEAIFTDVSDTIRKALMRTHAKNKST
                     THIARTAFDIALDELL"
     gene            2822..3535
                     /locus_tag="PK610_08565"
     CDS             2822..3535
                     /locus_tag="PK610_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013304691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRJ36027.1"
                     /translation="MFNIPILAQTDLGEAASTAASGATGIIETTKEITLWEMIQAGGW
                     YIMVPMAIMSVLLIYISIERFLAINKAARGENSFMQKVKEYIHEGKIDAARNLCATTD
                     NPYARMVEKGITKIGKPLKDIEASIENVGKVEVSKLEKGVSLLATIAGAAPMIGFLGT
                     VIGMIMTFNEMRIDGQVEIQALSGGIMMAMVTTVAGLILGIFSYIMYNMLVAKVGDVI
                     SKMETTSLEFLDLLDEPGK"
     gene            3554..3952
                     /locus_tag="PK610_08570"
     CDS             3554..3952
                     /locus_tag="PK610_08570"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRJ36028.1"
                     /translation="MNFRSRNKVAAEASMSSMTDLVFLLLIYFVILATQISPGINVDL
                     PKTRPSHMPPMAAPCVVSVDADQQHYVATIAVTTDELEEAMRKELEKNPEKPTVLMRI
                     DETVPTGVTVNILDICKRNEWKVGIATKSE"
     gene            4004..4780
                     /locus_tag="PK610_08575"
     CDS             4004..4780
                     /locus_tag="PK610_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36029.1"
                     /translation="MNDTEKDKKRKRKAMFATFVFHVGLLLVLFLVSWKEPFPLPEPK
                     SVEIEFMGGSGSEGGSPSSSNATTNPATSNPQEEIKTQTESEVEVPRGNSKVTSPNES
                     RPNNSQTTTTSTTTTTSNNTTANTNPFGGGNGEKGNSVGNNVGEGDKGTGGGNKGGDG
                     TGDGTHKGPGVAMKGRKQVASPIPDNPNYERGRVVFTIYVNRDGYVTKAIFDASGSTT
                     NDPDLVASCKKELIGKQIVNADQTAAEEQKGSYMFEFNVR"
     gene            4784..>4967
                     /locus_tag="PK610_08580"
     CDS             4784..>4967
                     /locus_tag="PK610_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HRJ36030.1"
                     /translation="MSYRQTLDFLYSQYPVFQRIGASAYKPDLNNTIALLEVVNKPQK
                     AFRSIHVAGTNGKGSTS"
BASE COUNT         1487 a         1090 c         1147 g         1243 t
ORIGIN      
        1 gtaccgaatt tctccaatcc ccagcatacc agggaattgt ttaccgacat acatacagaa
       61 accgaagcat cgattttgga gatttcctcc attgccaatg cataagaaac ggtgtccatt
      121 ccgccgccac cgtatttggg atcgaccatc atgcccatga aaccaagctc acccaattga
      181 cgaatctcat ctttggggaa acgcatttca cggtcgcggt caattacgcc gggcttcagc
      241 acattctgtg caaaatcgcg cgctgctgca cgtactgcca gttgttcttc ggtgtattca
      301 aaattcatat tgcttgtttt ggtgttaagg gtggtaaaat tagaattttt atcgtgattt
      361 ttgtcctgat tctttttcca tgaaatccat gcacatactc ggtttaatgt caggcacctc
      421 gcttgatggt ctggatatgg cattgtgcga attcagccac accgatgctt accactatcg
      481 cattgaggct acccggttca ttccgtacaa caagcaatgg aaaaaaaagt tggccgaggc
      541 aatgctgctt cattcaactg atcttcttgc attagatgcc gaatacggac aatggctcgg
      601 agaacaatgt gttgaattcc tgaaaagtca tgccggcatt ccctgcgatg cgatcgcttc
      661 tcatggtcat accgtatttc atcaaccgga aaaaggtttt tcactgcaaa tagggagcgg
      721 agaccacatt gcagccatca gcggaaaact tacggtgaac gatttcagga gcggagatat
      781 tgcactgggc ggacaaggtg ctcccttggt tcctattggg gatttgctct tgtttggcga
      841 atatgcaatg tgtctaaatt tgggcggaat tgccaacatt agcggcacaa gtgaaggaaa
      901 acgcagggct tttgatatca gcgtagccaa catggcattg aatcatcttg caggaaaatt
      961 aggcatggac tacgatgata aagggaattt ttcacgaaaa ggaacaactt tacctgcctt
     1021 gctaaagcaa ttaaacgaca tccccttttt tcagcaagat ggacccaaat cacttggaaa
     1081 agagtggttt gataaagaat ttctgcctct cattgaaaat tcggaatgcc cggctgagga
     1141 ttgtttaagc actgtttgtg aacacatcgc ttttcaactg tcaaatgcga tgaatcgttt
     1201 taaccccggc aaagtattgg ttacgggtgg gggcgcatat aacgactatc tgatcgcacg
     1261 cattcaggca catacgcaga acgaaattat cgtggcagaa aaggagttaa tttctttcaa
     1321 agaagcgctc ctgtttgcct ttttgggttg gcttcggatc aacgaatgca ctaacagtct
     1381 tgccagtgta acgggagctt cccgcgatgc ctgctgcggc gtaatccgga tggggaaaca
     1441 gtaagtcatc ccagtccagt tctcaacatt cttgtattga taaccacgta agcatttctc
     1501 gttccaaggg gtgtcattgc ctaactttgg ccaactacat cgtgtgataa aatgaaagtg
     1561 aaactcaaac aatttgagac caagcgtccg gaaatcgtat ttgaatggaa agatgctgaa
     1621 acggatgctg aaggctgggt ggtcatcaac tcccttcgcg gtggtgctgc cggtggcgga
     1681 acccgcatgc gtaagggact cgacaagcgt gaagtggaat cgctggcaaa aaccatggaa
     1741 gtaaaattca cagtagccgg tccacccatc ggaggagcaa aatcaggaat taatttcgat
     1801 cctgccgatc cacgcaaaaa aggggtgctc gaacgctggt acaaagccgt tgctccgttg
     1861 ttaaaaaact actacggaac aggaggcgac ctcaacgttg atgaggtgca tgatgtaatt
     1921 ccaattacca atgcgcttgg cgttttacat ccgcaggaag gcgttgtgaa tggacattac
     1981 ggtgccaatg aaacagataa aaagaaatcg atctccaatt tacaatcagg agtttcgaag
     2041 gttctgaacg atgtaaggta ttcgcccgat gttacccgga agtatgtggt agccgatatg
     2101 attaccggat atggcgttgc agaagccgtg aaacattttt acgcgattta cggaggcgat
     2161 gcaaaaggca aaaaagtaat cgttcaggga tggggcaatg tgggatcggc agcagcttat
     2221 tacctcgctc agcaaggagc aatcgttgta gcaatcatcg atcgcgatgg tggtctggtg
     2281 aatgaaaacg gattctcctt tcaggaaatc cgcaaactat tcatcgaaaa ggatggaaat
     2341 aaattaaagg cagataacgt tattcctttt gcagaactca ataaaacagt gtgggatgta
     2401 aaggcagata ttttcttacc ctgtgcggca tcgcgcctga ttacggaaga tcagttgaac
     2461 agaatgatta atgcaggtgt aaatgttgtt tccgccggag cgaatgttcc ttttgctgat
     2521 ccggaaatat tttttggtcc gatcggcgaa aaggcagatg agaaattaag tctgttaccc
     2581 gacttcattg ccaattgcgg aatggcccgg gtgtttgctt atctcatgca gcccactgct
     2641 accattaccg atgaagctat ctttactgat gtaagcgaca ccattcgcaa agcattgatg
     2701 cggacccatg caaaaaacaa gagcacaaca catattgcac gaactgcatt tgatattgcc
     2761 cttgatgaat tattgtaacc ctgaaccaaa aaccacgtaa ttaacccaac tttatacgac
     2821 catgttcaac attcccattt tagctcagac cgatttaggc gaagcagcat ccactgcagc
     2881 aagcggtgca accggaatta tcgaaaccac caaagaaatc accctatggg aaatgattca
     2941 ggcaggtggc tggtacatca tggtacctat ggcgataatg tcagtattgc ttatctacat
     3001 ttcaatcgaa cgctttctgg ccattaacaa agctgcccgt ggagaaaatt cctttatgca
     3061 aaaagtaaaa gaatacatcc acgaaggaaa aattgatgct gcacgcaatc tatgtgcaac
     3121 aaccgacaat ccttacgcac gaatggttga aaaaggaatt accaaaatcg gaaaaccact
     3181 caaggatatc gaagcttcca tcgaaaacgt tggtaaggtt gaagtaagta aacttgaaaa
     3241 aggcgtttca ttactcgcaa ccattgcagg tgctgcgcct atgatcggtt tccttggtac
     3301 cgtaataggt atgatcatga cctttaacga aatgcgtatc gacggacagg tagaaattca
     3361 ggcgctttcc ggtggtatta tgatggccat ggttacaacc gttgccggtt tgattctcgg
     3421 aatcttctcc tacattatgt acaacatgct tgttgcaaag gttggagatg taatctctaa
     3481 aatggaaacc acctcactcg aatttctaga tctattagac gaacccggta aataaaccgt
     3541 aaagcgcaaa gcaatgaatt tcagatccag aaataaagtc gccgcagaag ccagtatgtc
     3601 gtccatgacg gatctggtgt ttctgttgtt gatctacttt gtaattctcg ccacacaaat
     3661 tagtccggga ataaatgtag atctgccaaa aacgcgtccc tcgcacatgc ccccaatggc
     3721 agcaccgtgt gtggtgagtg ttgatgctga tcagcaacat tatgttgcta ccatagcggt
     3781 taccaccgat gaactcgaag aagcgatgcg gaaagagctg gaaaaaaatc cggaaaaacc
     3841 aaccgtattg atgcgtatcg acgaaactgt tccaaccggc gttacggtaa atatcctcga
     3901 tatctgtaag cgcaacgaat ggaaagtggg tattgccaca aaatcagagt aaactatatt
     3961 gaaccgatag cgtacaacag tgtacaggta aaaaaacaag gttatgaacg atactgaaaa
     4021 agacaaaaag agaaaacgaa aagcgatgtt cgctaccttc gtttttcatg ttgggttgtt
     4081 actggtactg ttccttgtta gctggaaaga accttttcct ttgcctgaac ccaaatcggt
     4141 agaaattgaa tttatgggcg gatcgggtag cgagggcgga agtccgagtt cttcaaacgc
     4201 aacaaccaat cctgcaactt ccaatccaca ggaagaaatt aagacacaaa ctgaatctga
     4261 agttgaagtc ccccggggaa attcaaaagt cacttctccc aatgagagtc gtcccaataa
     4321 ttcacaaact acaaccacat caacaactac caccacttcc aacaatacca ctgccaatac
     4381 caatcctttt ggcggaggaa acggtgaaaa agggaatagc gtaggaaata atgttggaga
     4441 aggcgacaaa ggcactggcg gtggaaataa aggcggagat ggaaccggcg acggcacgca
     4501 taaaggtccg ggtgtagcca tgaaagggag aaagcaggtt gcctccccta ttcctgataa
     4561 tccgaactac gaaagaggtc gcgtagtgtt taccatttat gttaaccgcg atggttatgt
     4621 taccaaggcc atttttgatg ccagtggttc aacaacaaat gatcccgacc ttgttgcttc
     4681 gtgcaaaaaa gaactgatcg gcaagcaaat tgtgaatgca gatcaaacag ctgctgaaga
     4741 acagaaaggt tcttacatgt tcgaatttaa tgtgcgctag cctatgagct accggcaaac
     4801 gctggatttc ctttattcgc aataccccgt atttcaacgc atcggtgctt cggcctacaa
     4861 acctgatctg aacaatacga ttgccctgct cgaagtggta aataaaccac aaaaagcatt
     4921 caggagcatt catgttgccg gcaccaatgg aaaaggctca acatcgc
//
LOCUS       DAOMYG010000242         4961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5871_length_4961_cov_8.386058, whole genome shotgun sequence.
ACCESSION   DAOMYG010000242 DAOMYG010000000
VERSION     DAOMYG010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5871_length_4961_cov_8.386058"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PK610_08585"
     CDS             complement(<1..308)
                     /locus_tag="PK610_08585"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HRJ36031.1"
                     /translation="MKAFISDPTKNTPEIRFDPSSARFTIRGKSYPENSRQFYAPVLQ
                     WIRMNRFPASSVLELEFDYISSSSVIAILEMLKMIEETDKSVKVIWTYEAGDDDLMNV
                     G"
     gene            complement(310..894)
                     /locus_tag="PK610_08590"
     CDS             complement(310..894)
                     /locus_tag="PK610_08590"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HRJ36032.1"
                     /translation="MSSSSNKVQEICNSRYQEIYGNYNSKSQKKILVSHFGDFTQDLV
                     NSISNGVEEGMLEAGDKKGTVKRMFSILVEGLQNVRLHGEKDEDGQQHSFLVIAVDES
                     EYLVTFGNLLLNSNRAIMDERLKQINGYDEAQVKALYMEVLTNGIISTKGGAGLGFIT
                     MAMKSKNKLNYTFEEVNDKLCCFTCEVKVDRNGG"
     gene            complement(1108..1935)
                     /locus_tag="PK610_08595"
     CDS             complement(1108..1935)
                     /locus_tag="PK610_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36033.1"
                     /translation="MKEFSLLRSFLLIALSLVLTVALAQEDEEQSSGSLMAGKFYFGI
                     NAGGYLANKHTSNYYAGYNDFTIPELIAIPQIYSQIYTILQSNFRMGEFPANPRYRTG
                     INLGLNLGYYLDESMALYSNIDIVRLKFQEVFTLEADNPANPSGDPIVYPQSIIGEEN
                     RMIIDLGLHFDFGDPGKPGGYFEIFGSFNATRARKNEVIIENNLKYSLLSQPNVYNRV
                     NLGGVGYGLGIGTGVRVKFNRQYTFDLGATAAFQKIALWDDRRLKLNPLIYVRLLWL"
     gene            1996..3174
                     /locus_tag="PK610_08600"
     CDS             1996..3174
                     /locus_tag="PK610_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRJ36034.1"
                     /translation="MGLWDKIKGQLIDIIEWLDTSDNSIAFRFERQGNEIKNGAKLIV
                     RESQVAVFQNEGEFADVFQPGTYTLETQNMPILSTLKGWKYGFNSPFKAEVIFVNTKV
                     ISDLRWGTKGAMTINDMSSPLFRNTQLKAFGSMEAKVVDPKLFIREVMGTDDHFTKEE
                     IVDRLRTRAVSFLTRVINSDTSLLPKFGSDEVNEKVTERMNAEFAKMGMMLNLFTVES
                     HEFDADTKARLERMSAAELKAFEIRVENQAKADAINAFSDMNKFQQFQAGNAMEAAAN
                     NPNSGDMGSMMGAGMGFAFGQTMMNQQNNANQQNRGGGAVPPPLPGQVKFFVAANGQQ
                     YGPYEMQQLQQMVQGGQLTRESMVWKEGMAGWLAAGTVPELSSIFGAVPPPPPPPPMP
                     "
     gene            3224..4330
                     /locus_tag="PK610_08605"
     CDS             3224..4330
                     /locus_tag="PK610_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36035.1"
                     /translation="MEEVLVDRADQKETKCKECGAILHYAPGTTALNCQYCGTENAFE
                     VKHEPVEEMDFEKFVHDFMQNESATQTIPTIHCDACGATTSFDPKIVSDSCAFCGSHL
                     TIKEGSTKLIKPKSLLPFGVDKNKAFEEFKKWVSSRWFAPNDLKQYLRHSEKLAGVYI
                     PYWTYDAATASQYTGMKGIHRTESYTSFENGKTVTKTRTVTDWYPCSGWVDRDFDDVL
                     VIGSTSLPKSYAEELEPWDLNNLVPFDEKFLSGFRAESYCVDLIKGYDDAKNKMDKVI
                     RRDVEKHIGGNAQRIITLNTTHSDITFKHILLPIYISAFQYSGKTYRFLVNGRTGEVQ
                     GERPYSTMKIVFFILAILAVIAGIVMLVMYFNNR"
     gene            4467..>4961
                     /locus_tag="PK610_08610"
     CDS             4467..>4961
                     /locus_tag="PK610_08610"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36036.1"
                     /translation="MRYSLLFGTFLFVQSLFGQNKYEIDLTAVSKDQITVKYTPSKKP
                     STDILRFSFPATVPGTYATQDYGLYVTTLTATDANGKKLPAKKAGNNTWVVSNAQQLS
                     SIVYSVEDAMDKQVKKNKIFEPASTNFMEGKLFFFNNGGIFGFEEGKENETLIVDIKN
                     PADLY"
BASE COUNT         1487 a         1060 c         1070 g         1344 t
ORIGIN      
        1 cccacgttca tcaggtcatc atcaccggct tcatacgtcc agattacctt aactgactta
       61 tcggtttctt caatcatttt aagcatctca aggatggcaa ttactgaaga cgaagagata
      121 taatcaaatt caagctcgag tacggaggag gcaggaaaac gattcatcct gatccattga
      181 agaaccggag cgtaaaactg acgtgaattt tcggggtaag attttccgcg tatggtaaac
      241 ctcgctgaag aggggtcgaa gcggatctca ggagtattct tggttggatc gctgataaag
      301 gctttcatgt taacctccgt tcctgtccac ttttacttca caagtaaagc agcacaattt
      361 atcattgacc tcttcgaaag tataattgag tttgttcttc gatttcatag ccatggtaat
      421 aaaaccaaga ccggcaccac cctttgtgga gatgattccg ttggtaagga cttccatgta
      481 caatgccttc acctgtgcct cgtcataacc gttgatctgc ttcagacgtt cgtccatgat
      541 tgcacggttg gaattgagca acaggttacc aaatgttacc aggtattcgc tttcatccac
      601 agcaataacg aggaatgaat gctgctgacc atcttcatct ttttcaccgt gtaatcttac
      661 gttctgaaga ccttctacca gaatagaaaa catacgtttt acagtacctt ttttatcgcc
      721 ggcttcgagc ataccctctt ctactccatt agaaatggaa ttcacaagat cctgcgtgaa
      781 atcaccaaaa tgtgatacca ggattttctt ctgggactta ctattgtagt tcccataaat
      841 ctcctgatac cgcgagttgc agatttcctg taccttattc gagctagaac tcatcagcgg
      901 tgtagcggat taacagaggg ttacgtgatt agcttgcaag ttaacaaaaa tccggataag
      961 caacaatcat cggtaaaaaa ttgagtttca acctcttttt agaaccatca gacgtttcag
     1021 cacctggatt tctgtcaaat ttaccattga actgcaattt tcgaaggaat tatgtgctaa
     1081 actattaaca attgatggtt aaacaaatca taaccaaagc agacgtacat aaatcagcgg
     1141 gtttagcttg agcctgcggt cgtcccaaag cgctattttc tgaaaagcag ccgttgcgcc
     1201 aaggtcgaaa gtgtactgac ggttgaattt tacacgaacc ccggtgccaa tacccagtcc
     1261 gtagcccact ccacccaggt tgacccggtt gtagacattc ggttgcgaca aaagcgaata
     1321 tttgaggttg ttttcaatga tgacctcatt cttccgggcc ctggttgcgt taaaggatcc
     1381 gaaaatctcg aaataaccac cgggtttgcc gggatcgccg aaatcgaagt gtaatccaag
     1441 atcgatgatc atccggtttt cctcaccaat tatggactga ggataaacga taggatcacc
     1501 tgaaggatta gcagggttgt cggcctcaag agtgaatacc tcctgaaact ttagccgtac
     1561 aatatcaata ttactgtaga gtgccatgct ctcatcgaga tagtagccca ggtttagtcc
     1621 cagattgata cctgttctgt atctgggatt agcaggaaat tcgcccatcc tgaaattgct
     1681 ttgcagaatg gtatagatct ggctatagat ctgagggatg gcaattaact cgggaatggt
     1741 aaaatcgttg taacccgcgt aatagtttga ggtgtgttta ttggcaagat accctccggc
     1801 attgattccg aagtaaaatt tcccggccat gagcgaaccc gacgactgct cctcatcctc
     1861 ctgtgccaac gccaccgtta ggaccaagct gagagcaatc agtaaaaaac ttcttaacaa
     1921 gctaaattcc ttcatcctat tatctatttc aggtttcaat attattacta ttttcatcaa
     1981 aaaatcattt tcgccatggg attatgggat aaaattaagg gtcaactgat agatattatc
     2041 gagtggctcg atacttcaga caactcaatc gccttccgtt ttgagcggca gggaaatgag
     2101 atcaagaacg gtgcaaagct gattgtgcgc gaatcgcagg ttgcagtttt tcagaacgag
     2161 ggtgaatttg cggatgtttt ccaaccgggc acctacaccc tggaaacaca gaacatgccc
     2221 attctttcta cacttaaagg atggaaatac ggttttaatt ctccatttaa ggcagaagta
     2281 attttcgtaa atacgaaagt catttccgat ttacgctggg gaaccaaagg agctatgacc
     2341 attaatgata tgtcgtctcc gcttttcaga aacacacagc ttaaagcgtt tggttctatg
     2401 gaagcgaaag tggtcgaccc aaaacttttc attcgtgaag taatgggtac cgacgatcat
     2461 ttcaccaagg aagagattgt tgaccgtttg cgtacaagag cagtttcctt ccttacaaga
     2521 gttataaaca gtgataccag tctgcttccg aaattcggaa gtgacgaggt taatgaaaag
     2581 gttaccgaaa ggatgaacgc agaattcgcc aagatgggaa tgatgctgaa cctgttcact
     2641 gtagaaagtc acgaattcga tgccgatacg aaagcacgtt tagagcggat gtcggctgca
     2701 gaactgaaag cgtttgagat ccgcgtggag aaccaggcga aggcagatgc tatcaatgca
     2761 ttttctgata tgaataagtt ccagcaattc caggcaggta atgcaatgga agcggctgca
     2821 aataatccga acagcggaga tatgggaagt atgatgggtg ctggaatggg atttgctttc
     2881 gggcaaacca tgatgaacca gcagaacaac gccaatcagc aaaaccgcgg aggaggagct
     2941 gttccaccac cactacccgg ccaggttaag ttttttgttg ctgcaaacgg acaacaatac
     3001 ggtccctacg aaatgcaaca attgcaacaa atggtacagg gaggtcagct tactcgcgag
     3061 tcgatggtgt ggaaagaagg aatggctgga tggcttgctg caggtacagt acctgaatta
     3121 agcagcattt tcggtgctgt tccaccgccg ccaccgccgc caccaatgcc ttgattttag
     3181 ggattgactt ttcattttag ttttgaatta aacagctttt ccaatggaag aagttctcgt
     3241 tgaccgtgcc gatcaaaaag aaaccaaatg caaagaatgt ggtgccattc tccactatgc
     3301 tcccggtact acggcattga attgccagta ttgcggaaca gaaaatgcat ttgaggttaa
     3361 acatgagccg gttgaggaaa tggattttga aaagtttgtg cacgatttca tgcagaatga
     3421 atctgcaaca caaaccattc caaccatcca ttgcgatgcc tgcggagcaa caacttcttt
     3481 cgacccgaaa attgtatccg attcctgtgc attttgcggc agtcacctta ccattaaaga
     3541 aggaagtaca aaactgatca aaccgaaaag tttacttcca tttggagttg acaagaacaa
     3601 agcattcgaa gaattcaaaa aatgggtgag cagcagatgg tttgctccaa acgatctaaa
     3661 acaatacctg cgtcattcag aaaaacttgc aggagtatat attccctact ggacctacga
     3721 cgctgcaact gcgagtcagt acaccggaat gaaagggatt catcgcacgg aaagttatac
     3781 cagttttgaa aatggaaaaa cggttaccaa aaccagaacg gttaccgact ggtatccctg
     3841 ctcaggttgg gttgatcgtg attttgacga tgtgctggtg ataggatcaa catcgctacc
     3901 aaaaagctat gcagaagaac tggagccatg ggatttgaat aatcttgttc ccttcgatga
     3961 aaaattccta tccggtttcc gtgcagaaag ttattgtgtg gatctgatta aaggatacga
     4021 tgatgcaaaa aacaaaatgg acaaagtgat tcgccgcgat gtggaaaaac acattggcgg
     4081 gaatgcacaa cgcatcatta cactcaacac cacgcattct gacatcacct ttaaacatat
     4141 tcttcttccc atttacatca gtgcatttca gtatagtggt aagacctacc gctttcttgt
     4201 taatggccga accggagaag ttcagggtga gcgtccgtac agcaccatga agattgtttt
     4261 cttcattttg gctatcctcg ctgttattgc aggaattgtg atgctggtaa tgtatttcaa
     4321 caaccgctga ggattgttaa acgaaacaat taccggaaaa caaccactta caattgcatt
     4381 ttaccgctta tgttgtgctt aaaacctttt tgcgggttga gcagtagatt tgcctaaaca
     4441 ttgaatctta cctgaaatcc ataaccatgc gttattcatt attattcgga acattccttt
     4501 ttgttcaatc actttttgga caaaacaaat acgagatcga tcttaccgct gtatccaaag
     4561 atcagatcac tgtaaaatat actccttcta aaaaaccaag tacagatatt ctgcgctttt
     4621 catttcctgc aactgttccc ggcacctatg ccacgcagga ttatggcctg tatgttacaa
     4681 cactcacggc gactgatgcg aacggaaaaa aactacctgc taaaaaagcc ggaaacaaca
     4741 catgggtggt aagcaatgca caacaattaa gcagtattgt ttattctgtg gaagatgcaa
     4801 tggacaaaca ggtaaaaaag aataaaattt ttgagcctgc atctaccaat tttatggaag
     4861 gaaaattatt cttttttaat aacggtggga tttttggttt tgaagaagga aaagaaaacg
     4921 aaacattgat tgtcgacatt aaaaacccag ccgatctgta t
//
LOCUS       DAOMYG010000243         4961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5872_length_4961_cov_7.439462, whole genome shotgun sequence.
ACCESSION   DAOMYG010000243 DAOMYG010000000
VERSION     DAOMYG010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5872_length_4961_cov_7.439462"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PK610_08615"
     CDS             complement(<1..484)
                     /locus_tag="PK610_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36037.1"
                     /translation="MKKIKFYKSGKFWKRIVLFGLGIPVLLFGILVAVLYWKQDAIVQ
                     YMIGQANKDFKGRVELKDSHVSLFANFPYISIDLEEVRIYEDKDEHDHPIVEVHDLYL
                     GFDIWTILSGNMEVKLIKMKDGVLNLVQHADGSFNVSRALETNEKKVEDPNEEFHLDL
                     K"
     gene            complement(540..2315)
                     /locus_tag="PK610_08620"
     CDS             complement(540..2315)
                     /locus_tag="PK610_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRJ36038.1"
                     /translation="MDHVKRLFDIPRHQLKSYPKDDMLTSKVNGKWVPWSTAKAIEHA
                     EWFSKGLLELGISKGDKIAMISNNRPEWNIADMGILQIGAVDVPVYPTASEKDYEFIF
                     NDAEVKICLVSGKELYDKVMLIRDKVPSMKEIYSFDPIEGVKSWNEILEKGKSASSNR
                     EAELKSTMESISEDDLATLIYTSGTTGVPKGVMLTHKNLVCNAIACISRLPVDSKGRS
                     LSFLPICHVYERMIHYLYMITGVSIYFAESIDTVGDNLKEVKPQVFVAVPRLLEKVYD
                     KIIAKGTDLSGIKKKLFFWAVDLGMQYEPGNRNNWWYRFRLKIARKLIFSKWQEALGG
                     NVLAVASGGAALQPRLARIFLAAGIPVMEGYGLTETSPVIAVNCEKNDGVRIGTVGRV
                     LDNVEVMIAQDGEILVKGPSVMKGYYKRPDATAETIDADGWLHTGDIGELVEGQFIRI
                     TDRKKEIFKTSGGKYIAPQPMENKFKESRFVEQVMIIGEGQKFPAALIVPAFVVVKEW
                     GARHGHHFSSNEEMANSPVVKARIMQDIEEFNKDFGNWEQVKKIALLPQEFTILSGEM
                     TPTLKLKRKFILEKYKDVVNGIYAE"
     gene            2699..3160
                     /locus_tag="PK610_08625"
     CDS             2699..3160
                     /locus_tag="PK610_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRJ36039.1"
                     /translation="MKEYAGRLHRFVFKSLRNTEDTRDIVQDTFAKLWVNRKKVEWEK
                     AKSWLFTVAHNGMLNFIKTRNRVELVEEYSQWEPHMEDELRMELKEIVDYCLDQLTPV
                     QRSVLLLRDLEGYEYKEISEILNLSESQVKVYLFRARQKMKSQLKDLTVLA"
     gene            3157..>4961
                     /locus_tag="PK610_08630"
     CDS             3157..>4961
                     /locus_tag="PK610_08630"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRJ36040.1"
                     /translation="MSRINHGNIDKWLFDHVEGNLSPSQQRELNSFLLEHPEYELDLD
                     AWKQSHISTEEEQIPVLAYESELLAIAEPFAWKRYAAVAALALLIGGGSILLLNQNES
                     TSENHTATLVNGSTSRFTAMIPNAANGNSIVLNSIVSVGNEQNTATTSPSFQTEATAI
                     SATSLPDRNRNQVRATQTSTVVFANSTRTPLSLNSTSEEQIAFASNEQALEQENTTLA
                     TNENRESNTETAIASANELPKLVHVFDAYRPELVSEAYGNTAAVTAIDILKGQPVDLN
                     NPGTPLASNEVRDNGVSQPRRKKFHDNRLGFYNGRDHLLLQPNNIATAEYAAFASTSV
                     SPSLSLGYRNQWSDADHSVNNARVFYSQFNKKMKSAWGIGMNAISANNGGYTGFTGSF
                     LFSPKFRINKNFSIEPGVMANIYQNDIHADKLHSGSRIEPVRGVFGMPLNGEGMPKTQ
                     RGFDVHFSTLVQTKYFYSAIGIDNMLHPALGVRQNDMGGVSSTPTRFKAVVGTDFKRL
                     YESKLVLSPQVSFMRQGQVNELWAGFNARYQWMSLGAAAAPNGQVLATAGIQTGRLRL
                     LYQFDVTKSYLNQTYYGSHEVSMRLSLNGLGKKQSV"
BASE COUNT         1437 a         1120 c          981 g         1423 t
ORIGIN      
        1 cctttagatc aaggtgaaat tcttcgttag ggtcttctac ttttttctca tttgtctcaa
       61 gggctcgtga aacattaaaa cttccatcgg cgtgctgaac caggttgagt acaccatcct
      121 tcattttaat aagtttcacc tccatatttc cggaaaggat ggtccagata tcaaagccca
      181 gatacaaatc atgcacttct acaatgggat gatcgtgttc gtccttatcc tcataaattc
      241 tgacttcttc caggtcgatg gagatgtatg ggaaatttgc aaataaagaa acatgagaat
      301 cctttaactc aaccctgcct ttaaaatcct tgttggcctg accgatcatg tattgtacaa
      361 tggcatcttg tttccagtac aatacagcta ctaaaattcc aaatagcaag accggaatcc
      421 ccaatccaaa caacacaatt cgcttccaga attttccgga cttatagaat tttatttttt
      481 tcattttaac acattaattc cggcatcacc ggtcaggata agagctcgct gaaaagattt
      541 tattcagcgt aaattccatt taccacatcc ttatactttt caaggataaa tttccttttc
      601 agtttaagtg tgggtgtcat ctcaccggat agaattgtaa actcctgagg caacaatgca
      661 atttttttga cttgttccca attcccaaaa tccttattga attcttcaat atcctgcatt
      721 atgcgggctt taaccacagg cgaattggcc atttcttcat tggaagaaaa atgatgtcca
      781 tgacgtgctc cccattcttt taccacaaca aatgcaggta caatcagtgc tgccggaaat
      841 ttctgacctt caccaattat catcacctgt tctacaaatc gtgattcctt gaatttattt
      901 tccattggtt gtggagcaat atattttcct cctgatgttt taaagatctc ttttttacga
      961 tcggtaatcc ggatgaattg tccttccacc aactcaccaa tgtcaccggt atgcaaccag
     1021 ccatcggcat caatcgtttc ggcagttgca tccggacgct tgtaatagcc cttcataacg
     1081 cttggacctt tcaccaaaat ttcaccatcc tgagcaatca taacctcaac attatccagt
     1141 acacgtccaa ctgtgccaat acgaacaccg tcgttctttt cgcaattcac tgcaattacc
     1201 ggtgaagttt cagtaagacc ataaccttcc attacaggga ttcctgctgc aagaaaaatg
     1261 cgtgcgagtc gtggttgcaa tgcggcacct cccgaagcta ctgccaacac attaccgcca
     1321 agtgcttcct gccatttgga aaaaatcagt ttacgtgcaa tttttagtcg gaagcggtac
     1381 caccaattgt ttctgttacc tggttcgtat tgcataccca gatccacggc ccagaagaaa
     1441 agttttttct tgatgccact gagatccgtt ccttttgcga tgattttatc atacactttt
     1501 tcaagcaatc gaggtacggc aacaaatacc tgtggtttta cctccttaag gttatcgcca
     1561 acggtgtcga tgctttcagc aaaataaatg gaaacgccag ttatcatgta gaggtaatga
     1621 atcatgcgct cgtaaacgtg acaaatcggt aagaagctaa gtgatctgcc tttgctgtca
     1681 accggaagtc gggaaataca tgcaattgca ttacaaacca gatttttgtg tgtaagcatt
     1741 acccctttgg gaacaccggt ggttccactg gtataaataa gcgtagccag gtcatcttcg
     1801 gaaatactct ccatggttga tttcagttca gcttcgcggt tggaggatgc tgattttcct
     1861 ttttctaaaa tttcattcca cgatttgact ccttcgatgg gatcgaaaga ataaatttct
     1921 ttcatggaag gaactttatc gcgaatcaac attaccttat cgtagagttc tttaccggaa
     1981 accaagcaaa tttttacttc ggcatcgtta aaaatgaatt cgtaatcttt ttcggaagca
     2041 gtgggataca ccggaacatc tactgcacca atctgcagaa tacccatatc ggcgatgttc
     2101 cattccggac gattattcga aatcattgca attttatcgc ctttggaaat tcccaattcc
     2161 agtaatcctt tactgaacca ttcggcatgt tcaatggcct ttgcggttga ccatggcacc
     2221 catttcccat taacttttga ggtaagcatg tcatccttgg gataggattt caactggtgg
     2281 cggggaatgt cgaataagcg ttttacgtgg tccatggcgt tcaaatgttg agtgattgct
     2341 taaaaataag caacaaacct cacttttcgg acgatttcat cgatttgagg attttggaaa
     2401 tccagcttaa aacaaaaact actcataatc aatgtattaa acaaagtctt ttaatgagaa
     2461 tgttttcaaa cggttgatta tcaaaaacct aaactttata aatagttaat tttttttacc
     2521 attttttttc ctgctattcc ttgaaattga gtgcttttca ttatctttcc ctaaagaaat
     2581 acggaggtcc taccgttttt tctgcgggcc tgtaactttt gggggtatct catggttaca
     2641 cgtttcaaag gaccttcagc aaacgaacaa cttgaaccgg gcagagtacc atagagccgt
     2701 gaaagagtac gcaggacgat tgcatcgttt tgtgttcaag agtctgcgca acaccgagga
     2761 cactcgggat attgtgcagg acacttttgc gaagctttgg gtaaaccgga aaaaagttga
     2821 atgggagaaa gccaaatcct ggctcttcac tgttgcgcac aatggcatgt tgaattttat
     2881 caaaacccgc aatcgcgtgg aattggtgga ggaatacagt cagtgggaac cgcacatgga
     2941 agatgagtta aggatggagc tgaaagagat cgtagattat tgtctcgatc agcttactcc
     3001 ggttcagcgg tctgttttat tgctgaggga tcttgaaggc tacgaataca aagagatcag
     3061 cgaaatcctg aacctttcag agagtcaggt taaagtgtat ctcttccgtg cccgtcagaa
     3121 aatgaaatca caactcaagg acctaaccgt attagcatga gcagaatcaa ccatggcaac
     3181 atcgacaaat ggttgttcga tcacgttgaa gggaatttat ccccatcgca acaacgagaa
     3241 ctgaacagtt tccttttgga gcatcccgag tatgaactcg atctggatgc ctggaaacaa
     3301 tcccatattt ccaccgaaga agaacaaatt cctgttcttg cttacgaaag tgaattactc
     3361 gcaattgcag aaccatttgc atggaagcgt tacgcagctg ttgcagcact tgcattacta
     3421 attggaggtg gaagtattct gttgcttaac caaaatgaat caacttcaga aaatcatacg
     3481 gcaacacttg taaatggaag tacttctcgc ttcactgcaa tgattccaaa tgcagctaac
     3541 ggaaacagta ttgtattaaa ttctatagta tctgtcggca acgaacagaa caccgcaact
     3601 acttcaccat cctttcaaac tgaagcaaca gcaatttccg caacgagctt gcctgaccgc
     3661 aaccggaatc aagttcgcgc aacacaaacg agcactgtgg tatttgcaaa ttcaacccgc
     3721 actccgctta gtctgaactc caccagtgaa gaacaaattg cattcgcttc taacgagcaa
     3781 gccctggagc aggaaaacac aacattggca acaaatgaaa accgggaaag caatacagag
     3841 actgcaatcg catctgcaaa cgaacttcca aaattagttc acgtatttga tgcttaccgt
     3901 ccggaacttg tctctgaggc ttacggaaac actgctgcgg taactgcaat tgatattctg
     3961 aaaggtcaac cggttgattt gaacaatcct ggaaccccac ttgcttcaaa tgaagttcga
     4021 gacaatggag tttcacaacc tcgtcgtaaa aagtttcatg ataaccgtct tggtttttac
     4081 aatggcagag atcatcttct cctgcaaccc aacaatatcg ccactgccga atatgcagca
     4141 tttgcatcta ccagtgtgag tccttcatta agtcttggtt accggaatca atggagtgat
     4201 gcagaccaca gtgtaaacaa tgcccgtgtt ttttattctc agttcaacaa gaaaatgaaa
     4261 agtgcatggg ggataggaat gaatgcaatc agtgcaaaca acggaggcta taccggattc
     4321 accggatcat tccttttctc tccgaagttt cgcatcaata aaaatttcag tatcgaaccc
     4381 ggagtgatgg ctaatattta tcagaacgat attcatgccg acaaactcca ttccggaagc
     4441 cgaattgaac cggtacgcgg agtattcgga atgcctctta atggtgaggg aatgccaaaa
     4501 acccagcgtg gatttgatgt gcatttctct acgctcgtac aaacgaaata tttctattcc
     4561 gcaatcggaa tcgataatat gttgcatccc gctctaggag ttcgccaaaa cgacatgggg
     4621 ggtgtaagct ccacccctac ccgattcaaa gccgtagtgg gaaccgattt caaacgcttg
     4681 tacgaatcga aacttgtact atcacctcag gttagcttta tgcgtcaggg tcaggtcaat
     4741 gaattatggg caggattcaa tgcacgttat cagtggatga gcttaggtgc tgctgcggct
     4801 cctaacggac aagttcttgc aactgcagga attcaaaccg gacgccttcg tttgttgtac
     4861 caattcgatg ttaccaaatc atatctgaac cagacctatt acggttcaca tgaagtttcc
     4921 atgcgccttt ctctgaatgg tcttggtaaa aaacaaagtg t
//
LOCUS       DAOMYG010000244         4940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5906_length_4940_cov_9.191812, whole genome shotgun sequence.
ACCESSION   DAOMYG010000244 DAOMYG010000000
VERSION     DAOMYG010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5906_length_4940_cov_9.191812"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..659
                     /locus_tag="PK610_08635"
     CDS             108..659
                     /locus_tag="PK610_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018943367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WcaF family extracellular polysaccharide
                     biosynthesis acetyltransferase"
                     /protein_id="HRJ36041.1"
                     /translation="MKKTTDLSTYSNSWYHPGSAVKRVVWYGVNALFFLNPLFPFSGA
                     KVFFLKLFGAEIGTGVVIKPGVNIKYPWKLKIGDHVWIGEKVWIDNLGQVNIGDHCCL
                     SQGAFLLCGNHDYTKTGFDLIVKDITLENGVWIGAKAIVAPGTVLHSHAVLTAGSMAS
                     GELEAYSIYGGNPAQKIRERTIG"
     gene            656..1411
                     /locus_tag="PK610_08640"
     CDS             656..1411
                     /locus_tag="PK610_08640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRJ36042.1"
                     /translation="MKVSIITVCYNSAATIGDAIQSVVSQNHPDIEHIIIDGGSGDDT
                     LKIINQYKNNIAHLVSEPDKGIYDAMNKGIALASGEIVGILNSDDLYADENVVRDVIA
                     LFQKENCEGVYADLVYVDRENTSKINRYWKAGTYKKGAFRKGWMPPHPTFFVKRSVYG
                     RLGNFTTALKSAADYEIMLRFIHKHEISLVYLPRVIVRMRAGGKSNVSLRNRIRANRE
                     DKQAWVMNGLRPGLFTLIRKPLSKVFQFIKKGR"
     gene            complement(1445..2374)
                     /locus_tag="PK610_08645"
     CDS             complement(1445..2374)
                     /locus_tag="PK610_08645"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRJ36043.1"
                     /translation="MKTVMSNKLFGMFALLLVAQTGLAQRSSFYKFEVGGGLGMSNYL
                     GEIGGKEKTERPFVWDMKLNQTRWAVGGYARYKMSNYLSVRADLIYGRIQGADSLSDN
                     RGRVGRNLSFRNDLLEFSGRAEVYMYNTNDVGGKGWYRVDFKSYVFAGIGGVLHGPKA
                     LHNGEWVKLRPLTTEGVNYGRVTATMPIGVGFFFTYKRRHRFGWEMGWRMTFTDYLDD
                     ISGVYADPSMLPNATSIALANRSDELTDAQRPHIANYAPGQKRGDSNDNDTYFFTTFT
                     YGYVFRGKNPFYTQHYSWLGGRKGRRTISRVKF"
     gene            2466..3425
                     /locus_tag="PK610_08650"
     CDS             2466..3425
                     /locus_tag="PK610_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRJ36044.1"
                     /translation="MDNNKICSLFNIQYPLIQAGMIWCSGWELASAVSNAGGLGIIGS
                     GSMYPEVLKEHVKKCKAATNKPFAVNLPMLYPDIDKHIQTIIEENVPIVFTSAGNPKT
                     YTKMLKDHGKVVVHVVSSSKFAQKAQEAGVDAVVAEGFEAGGHNGREETTTLCLIPLV
                     RNAVSIPVIAAGGIANGRQMLAAMIMGADAVQVGSRFVASEESSAHANFKKAVVDAKE
                     GDTVLTLKEITPVRLIKNHFYQKVEEAYSRCAEPEELKELLGRGRAKKGMFEGDLNEG
                     ELEIGQIAAMLNEVKPAATIVREMMTEYNEALREWKQQSEYKI"
     gene            3427..4665
                     /locus_tag="PK610_08655"
     CDS             3427..4665
                     /locus_tag="PK610_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRJ36045.1"
                     /translation="MIQFERFTLANGLRVLVHRDESTPIVAFNLLYDVGARDEDEKRT
                     GFAHLFEHLMFGGSVNIPDFDKPLHAVGGTSNAFTNNDITNYYVTLPVDNLETAFWLD
                     SDRMLSLAFSEKSLEVQRNVVIEEFKQRYLNQPYGDVWLLLRPLAYAEHPYKWATIGK
                     EIKHIEDATLQDVRDFFGTHYIPSNGILCVAGNITTEQIKTLSEKWFGPIPAKTRKER
                     KLPKEPTQTEARSLTVKRDVPYPAFYRAWHMDKRDSESFYACDLMSDILSRGKSSRLY
                     DQLVREKKLFSEINAFITGDLDEGLFVVSGKLNEGVKMEDAETGVELALDELRTTLIP
                     ENELEKVKNKVETTKAFNDMSVLDKAMELCYFELLGDANRVNHEIDRYKKVTAADILK
                     VAQSILRKENSSTLYYYPNH"
     gene            4667..>4940
                     /locus_tag="PK610_08660"
     CDS             4667..>4940
                     /locus_tag="PK610_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36046.1"
                     /translation="MQIDRTIAPEAHAIKNVQLIRPQTQVLSNGIPFFALRAGSQQVL
                     RLEFIFDAGIRKQNRSLIASITNAMLVEGTPTKTAQQIAEMFDYYGA"
BASE COUNT         1524 a         1112 c         1108 g         1196 t
ORIGIN      
        1 ggaaaaattc accgctacac cggaaatgaa tcgtttcaca ccggagatct gttgtttatc
       61 ggcagcaagc atcaggacag aactgcacca tttctttcca gataacaatg aaaaaaacga
      121 ccgatctttc aacctattcc aacagttggt accatcccgg atcagcagtt aagcgcgttg
      181 tatggtatgg tgtaaatgca ctattttttc ttaatccctt atttccattc agcggagcaa
      241 aagttttctt cctcaaatta ttcggagcag aaatcggaac gggtgttgta attaaacccg
      301 gtgtgaatat caaatatccc tggaaactga aaattggcga ccatgtctgg atcggcgaaa
      361 aagtttggat cgacaatctt ggtcaggtaa acattggcga tcactgttgt ttatcgcaag
      421 gtgcattttt gctctgtgga aatcacgact acacaaaaac tggtttcgac ctgatcgtaa
      481 aagacatcac actcgaaaac ggagtatgga ttggcgcaaa agcaattgtt gctccgggca
      541 ctgttctgca ttcgcacgct gtacttaccg caggctccat ggcaagtggt gaattggaag
      601 cctactccat ttacggaggg aatcccgcac aaaagatccg tgaacggact atcggatgaa
      661 agtcagtata ataaccgtat gctacaacag cgcagcaacc attggtgatg ccattcaatc
      721 tgttgtttcg caaaaccatc cggatattga acacatcatt attgacggag gctccggtga
      781 tgatacatta aaaattatca atcagtacaa aaacaacatt gcacatctgg tttctgagcc
      841 cgacaaagga atttacgatg ccatgaacaa aggcattgca ttggcaagtg gagaaattgt
      901 aggcattctg aattccgacg atctgtatgc cgatgaaaat gtagtgcgcg atgttatcgc
      961 attgtttcag aaggaaaact gcgaaggcgt ttatgccgac cttgtttatg tagatcgcga
     1021 aaacacttcg aagatcaacc gttactggaa agcgggcacg tacaaaaaag gagcatttcg
     1081 aaaaggatgg atgccaccac acccaacgtt ttttgtgaag cgatcggtgt atgggcgact
     1141 cggtaatttt accacagcat taaagagcgc tgcagattat gagatcatgt tgcgctttat
     1201 tcataaacat gaaatttcac tggtctatct tcccagagta attgtaagga tgcgggcagg
     1261 aggaaaaagc aatgtcagcc ttcgcaacag aatacgtgca aacagagagg ataaacaagc
     1321 atgggtcatg aacggacttc gtcccggatt atttacgctt atccgaaaac cgctctccaa
     1381 ggtttttcaa ttcataaaaa aaggccggta attccggcct tttccatttt gtattttttc
     1441 tgaatcagaa tttcacacgg gaaatcgttc tgcgtccttt tcttcctccc aaccaggaat
     1501 agtgctgcgt atagaacgga ttcttaccgc ggaacacata accataagtg aaggtggtaa
     1561 agaaataagt atcgttgtca ttggaatcac cacgcttctg accgggcgca taatttgcga
     1621 tatgcggacg ctgagcatcg gtcaattcat cagaacggtt agccaatgca atagaagtag
     1681 cgttaggcaa catggatgga tcggcataca caccgctaat atcatcgagg taatctgtaa
     1741 atgtcattct ccaacccatc tcccaaccaa agcggtggcg acgtttatag gtgaaaaaga
     1801 atccaactcc aattggcatg gtagcggtta ctctgccgta gttcacaccc tcagtagtaa
     1861 gcggacgaag ttttacccat tcaccattgt gcagcgcttt cgggccatgc aatactccac
     1921 caattcctgc aaaaacgtag gatttaaaat cgacacggta ccatccttta cctccaacat
     1981 cgttggtatt gtacatgtac acttcggcac gaccggaaaa ttcgagcaaa tcattgcgga
     2041 aactcaggtt acgaccaaca cgtccacggt tatcggaaag actatcggct ccctgaatac
     2101 gaccatagat caagtcggca cgtacagaaa gataattgct cattttgtag cgggcatatc
     2161 caccaacagc ccaacgggtc tggttcagtt tcatatccca aacaaaggga cgctccgttt
     2221 tttccttacc gccaatctca ccaaggtaat tcgacatacc aaggccacca cccacttcga
     2281 acttgtaaaa tgaactacgc tgagctaaac ccgtttgggc aacaagcaac agcgcgaaca
     2341 ttccgaagag tttattggac ataactgttt tcatattcaa gcttcaaata tagagaatgt
     2401 ttcgaaagca ggcaagtcgt gtgccacagc caaaaagcct tagttttaca ccataaattc
     2461 atgccatgga caataacaag atctgctccc ttttcaatat ccaataccct cttattcagg
     2521 ccggaatgat ctggtgctca ggctgggaac tcgcttctgc ggtgagtaat gcagggggat
     2581 tgggaatcat cggatcgggc tcgatgtacc ccgaagtgtt aaaagaacat gttaaaaaat
     2641 gcaaggcagc caccaacaaa cctttcgcgg tgaacctgcc catgctctac cccgatatcg
     2701 acaaacacat tcaaaccatc attgaagaaa atgtccccat cgtttttacc tctgcaggga
     2761 atcccaagac ctataccaaa atgctaaagg atcatggcaa agtggtagtg catgtggtga
     2821 gcagttcaaa attcgcccaa aaagcccagg aagccggtgt tgatgcagtg gtagcagaag
     2881 gatttgaagc cggtggtcac aatggaagag aggagaccac cactctttgt ttgatacccc
     2941 tggtaagaaa tgcagtgagt attccggtaa ttgccgcagg aggaattgcg aacggacgac
     3001 aaatgcttgc tgcaatgatc atgggagcag atgccgttca ggtgggaagc cgatttgttg
     3061 catcagaaga atcatcggcg catgccaatt tcaaaaaagc agtggtcgat gcaaaagaag
     3121 gagataccgt tttaaccctt aaagaaatta ctccggtacg cctgataaaa aatcattttt
     3181 accagaaggt agaagaggcc tattcccgct gtgccgaacc ggaagaattg aaagaattat
     3241 tgggaagagg aagagcaaaa aaaggaatgt ttgaaggaga cctgaatgaa ggggaactgg
     3301 aaataggaca aatcgctgcc atgcttaacg aagtaaaacc tgcagcaacc attgttcgtg
     3361 agatgatgac agaatacaac gaagcattgc gcgaatggaa acaacaatct gaatacaaaa
     3421 tctgatatga tacaatttga acgttttaca cttgcaaacg gattaagagt actggtgcac
     3481 cgcgatgagt cgacacccat tgttgctttc aatctactct atgatgttgg tgcccgcgat
     3541 gaagatgaaa agcgaaccgg atttgcgcat cttttcgagc acctgatgtt tggcggaagt
     3601 gtaaatattc cggatttcga taaacccttg catgccgtag gcggaaccag caatgcgttt
     3661 accaataacg acatcaccaa ttactatgtg acgctgccgg tagataatct tgaaactgca
     3721 ttctggctgg attccgaccg catgctcagt ttggctttct ccgagaaaag tctggaagta
     3781 cagcgcaatg ttgtgatcga agaatttaaa caacggtacc tcaatcaacc ttacggcgat
     3841 gtatggttgc tgcttcgtcc gcttgcctat gcagaacatc cctacaaatg ggcaacgatc
     3901 ggaaaagaga tcaaacacat tgaggatgcc accctacagg atgtgcgtga ttttttcggg
     3961 acacactaca ttccctctaa cggaattctt tgtgttgccg gaaatataac cactgaacaa
     4021 ataaaaacgc taagcgaaaa atggttcgga cccattcctg ccaaaacacg aaaagaacgt
     4081 aaacttccca aagaacccac acaaaccgaa gcaagaagtt taacggtaaa aagagatgtc
     4141 ccctaccctg ctttttacag agcctggcat atggataaac gcgattcgga atcgttttac
     4201 gcctgtgatc tgatgtcgga tattctttcg cgcggtaaat catcgcgact ttatgaccag
     4261 ctggtgagag agaaaaaact attcagcgag atcaatgcat ttataaccgg agatttggat
     4321 gaaggtttat ttgttgtaag cggaaaactg aatgaaggtg taaaaatgga agatgcggaa
     4381 accggtgttg aactggccct cgacgaactg cgcactacac tcattccgga aaatgaactg
     4441 gaaaaagtga agaacaaagt ggaaacaaca aaagcattca atgatatgag tgtgcttgac
     4501 aaggccatgg agttgtgtta tttcgaattg ctgggcgatg caaaccgtgt caatcacgaa
     4561 atagaccgct acaaaaaagt gactgctgcc gatatcctca aggtggcgca atccattctt
     4621 cgcaaggaaa acagttcaac gctttactat tatcccaacc actgatatgc aaatcgaccg
     4681 caccattgcc cctgaggcac acgcaataaa aaacgtacag ctgatccgac cgcaaacgca
     4741 ggtattgtcg aacggaattc ccttcttcgc gcttcgtgca ggatcgcagc aggttcttcg
     4801 tctggaattc attttcgatg caggaatccg caaacaaaac cgctcgctga tcgcatccat
     4861 taccaacgct atgctggtgg agggaacacc aacaaaaacg gcacagcaaa tcgcagaaat
     4921 gttcgattat tacggcgcat
//
LOCUS       DAOMYG010000245         4937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5916_length_4937_cov_8.273249, whole genome shotgun sequence.
ACCESSION   DAOMYG010000245 DAOMYG010000000
VERSION     DAOMYG010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5916_length_4937_cov_8.273249"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PK610_08665"
     CDS             <1..357
                     /locus_tag="PK610_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36047.1"
                     /translation="LNYYGLFGVSAGIKVKSRVDEDGKLFGTNNAASNDAMDNSKDMG
                     FFRAGVNIGGGAEWNLSGTTSLTFGLQYNMGFTNVVKKDSEYLYKYSSTPTPAFTSLN
                     QKFSSSYFGLVVGVLF"
     gene            414..1232
                     /gene="nadE"
                     /locus_tag="PK610_08670"
     CDS             414..1232
                     /gene="nadE"
                     /locus_tag="PK610_08670"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRJ36048.1"
                     /translation="MQHSRVILHISNWLKDYSNLSNTNGFVVGISGGIDSAVTSVLCA
                     LSGKKVVLVNMPIHQREQEYSRAKNHIENLCQRFSNVSYCEVSLTDAFEQLRKSMPDP
                     TSGHFLAMANTRARLRMTTLYAIGQTEGLLVAGTGNKIEDFGIGFYTKYGDGGVDLSP
                     IADLTKTQVFALAKELEVIAEIRNAKPTDGLWEDGRSDEDQIGATYPELEWAMEYVNR
                     FSGSSVNRIDPEFVPKNGLDDHQQKVLAIYIRLHNANKHKMLPIPVCEIPQELF"
     gene            complement(1323..2078)
                     /locus_tag="PK610_08675"
     CDS             complement(1323..2078)
                     /locus_tag="PK610_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36049.1"
                     /translation="MKKELIVFILLQLFAGVGNVFSQQEVRIYFFDGLSFSPYPEIRI
                     KIDHNSDTKILIPDSSGAVVLNRSGIEITYNDNNGKMRLWCRTIAPGSDSVVIKSNSG
                     KNKISGGFPLDGKLEIYVLRESEEFVLLKEITIRNRFPEKNPALAIQKGVEDKKSVEA
                     NKETDKGKTEIQAAGTVYRVQILTSGKLLTATDPLFKGEKEVWTYQDNGIYKYTVGNF
                     NTIGEAAQMQELMRQKGFKGAFVVKFVDGVRVK"
     gene            2165..3361
                     /locus_tag="PK610_08680"
     CDS             2165..3361
                     /locus_tag="PK610_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRJ36050.1"
                     /translation="MTASIIIAFCSLLLLAYVFDLTSSKTRIPTVILLLLLGWGVRQA
                     TDLLSFEIPDLNPLLPILGTIGLILIVLEGSLELELGKDKLPMIRKSIFGAVLPLVAL
                     TVLIAFAFNYFFEASLKDSITNAIPFCIISSAIAIPSVRNLRKENREFIIYESSLSDI
                     IGVLFFNFFAYNAVIQMEAVGYFAFEIILMILISFIVTIGLSFLLSKIDHHIKFFPII
                     LLIVLIYALSKIYHLPGLIFILIFGLFANNIQLLKTNKWISKFNPEGLSKEVDKFTEL
                     TIEATFMVRSSFFLLFGFLIETKEVIDIHTLPWSVSIIVSIILMRIVQLKLSKLPIMP
                     LLFVAPRGLITILLFLSIDPSSKIPMVNNSMVIQVVILSALLMMFGLMIHKKVPEEGE
                     QSNAEL"
     gene            complement(3367..3693)
                     /locus_tag="PK610_08685"
     CDS             complement(3367..3693)
                     /locus_tag="PK610_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013549819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRJ36051.1"
                     /translation="MAENTITVTVVDREGKEHVLEAPTDMGMNLMELCKSYELPVEGT
                     CGGMAMCATCQCYLISDTALPEQSDDELAMLDQAFHVKSNSRLGCQIKLSDEIDGIKV
                     ELAPES"
     gene            complement(3701..4720)
                     /locus_tag="PK610_08690"
     CDS             complement(3701..4720)
                     /locus_tag="PK610_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRJ36052.1"
                     /translation="MNSPIETDIIIVGAGPCGLFTVFEAGLLKLRCHLIDSLPQAGGQ
                     CSEIYPKKPIYDIPGFPSVLAGELVDNLMEQAAPFKPGFTLGESAEHVEKREDGKFVV
                     TTNKGTQHIAPIVAIAGGLGVFEPRKPPIPNITDFEDKGVEYIIKDPEFYRGKRVVIA
                     GGGDSALDWTIYLIDKKIAAHVTLVHRSQTFRGHLDSVQRVFDLAAEGKLNLVLDGEV
                     VGLKGGSSVEQVVIQQKDKGEYDLATDHFIPLFGLTPKLGPIANWGLEIEKGSIKVNT
                     FDYSTNIPGIYAIGDVNTYPGKLKLILCGFHEGTLMVQSAFKRIYPDKNLVLKYTTVN
                     GVNGF"
BASE COUNT         1404 a         1077 c          936 g         1520 t
ORIGIN      
        1 cttaactatt acggtttatt cggtgtttct gccggaatca aagtaaaatc aagagtagac
       61 gaagacggaa agctatttgg tacaaacaat gctgcttcaa acgatgccat ggacaacagc
      121 aaggacatgg gctttttccg tgcaggtgta aacattggcg gaggtgctga atggaacctc
      181 agcggtacaa ccagcctaac tttcggtctt cagtacaata tgggattcac caacgtggtt
      241 aaaaaagatt ctgagtatct gtacaaatac tcttccacac caactcctgc ctttacctca
      301 ttgaaccaga aattctcttc cagctatttc ggtttggttg ttggagtttt attctaatat
      361 tcttcgaaat cctctacctt tatcccgctc cccatacagg agcgggattt tttatgcagc
      421 attccagagt catcctccat atcagtaact ggttaaaaga ttattccaat ctatccaaca
      481 ccaatggatt tgttgtgggt atttccgggg gaattgattc tgctgttaca tctgtactct
      541 gcgcattaag cggaaaaaaa gtggtgttgg tcaatatgcc tattcatcag cgcgaacagg
      601 aatacagccg ggcaaaaaat catattgaga atctttgtca acgattttca aatgtttcgt
      661 attgtgaggt gtcactcacc gatgcgtttg aacaattacg taagtcgatg cctgatccaa
      721 cttccggtca tttccttgcc atggcgaata cacgtgcacg tttacgcatg acaacgctgt
      781 atgccattgg tcagaccgaa ggacttttgg ttgccggaac cggtaataag attgaagatt
      841 ttgggattgg attctatacc aagtatggcg acggcggtgt tgatcttagt cctattgctg
      901 atctcaccaa aacacaggtt tttgcgcttg caaaagagct tgaagtgatc gcggaaatcc
      961 gaaatgcaaa acccaccgac ggattatggg aagatggtcg atcagatgaa gaccagattg
     1021 gagcaactta tccggaattg gaatgggcga tggaatatgt aaatcgcttt tcaggaagtt
     1081 cagttaacag aattgatccc gagtttgtac ccaaaaacgg actcgatgat catcagcaaa
     1141 aagtgcttgc tatatatatc cgtctgcaca atgccaataa gcacaaaatg cttcctattc
     1201 ctgtttgtga aatcccgcag gaacttttct gatggatcgc atccccaaat ggcttaagct
     1261 aattattgtg tgtattattt gcctgggttt gatctctaca attgtgatcg tgttaagctg
     1321 agctatttta ctcttacgcc atctacaaac ttcacaacaa aagctccttt aaatcctttt
     1381 tgccgcatca gttcctgcat ttgcgcagct tcaccaatgg tattaaaatt tccaactgtg
     1441 tatttataaa ttccattatc ctggtaagtc caaacttcct tttcgccttt gaacaaagga
     1501 tctgtggcag taagcaattt acctgaagtc agaatctgaa cccgataaac agttcctgca
     1561 gcttgaattt ctgttttccc tttgtctgtt tccttatttg cttcaacaga tttcttatcc
     1621 tcaacgcctt tttgaatagc aagagcagga ttcttttcag gaaaacgatt tctgattgtg
     1681 atttccttta acagaacaaa ttcttcgctt tcacgcaaaa cataaatctc caatttaccg
     1741 tcaagcggaa atccacccga aattttattc ttaccggaat ttgattttat caccaccgaa
     1801 tctgatccgg gagcaatggt gcggcaccat aaacgcattt ttccattgtt atcgttatag
     1861 gtgatttcaa ttccggaacg atttaacact actgctccgg aagaatcagg aataagaatt
     1921 tttgtatctg agttatgatc aattttgatt ctgatttccg ggtagggaga aaatgataat
     1981 ccatcaaaaa aataaatacg tacttcctgt tgtgagaaaa catttccaac tccggcaaag
     2041 agttgcagca gtataaaaac aatcaattct tttttcacaa ttctactaaa ccactttttt
     2101 caacaattga gcatctaaaa tcgtatttta gttgttcagt tacaacacca actatcaaca
     2161 attaatgacc gcaagcatca taatcgcttt ttgcagtttg cttcttttgg catatgtctt
     2221 tgatctgaca tcatcaaaaa cgagaattcc aaccgttatt ttactgctac tgctcggctg
     2281 gggtgttagg caggcaacgg atttgttatc cttcgaaatt cccgacctga atccattact
     2341 ccctattctc ggaacaattg gtttgatttt aatcgtattg gaaggatcat tggagcttga
     2401 gttgggcaag gataagctcc cgatgatcag aaagtcgatt tttggcgctg tattaccatt
     2461 ggttgcgctt acagttctta ttgcatttgc attcaactat ttttttgagg ccagtttaaa
     2521 agatagtatt accaacgcaa tacccttctg tattatcagc agtgcgattg caattccttc
     2581 agtaagaaac ctgcgaaaag aaaacaggga atttatcatt tacgaaagca gtctgtctga
     2641 tattatcggt gtgttgttct tcaatttttt tgcatacaat gccgttattc aaatggaagc
     2701 cgtgggttat ttcgcatttg aaatcatact aatgattttg atttctttta ttgtaacaat
     2761 tggcttgtct tttctgctga gcaaaataga tcatcacata aaattcttcc caattatttt
     2821 acttattgtg ttgatctatg cactttcaaa aatctaccat cttcccggtt tgattttcat
     2881 cctgattttt gggcttttcg ccaacaacat ccaattgctc aagaccaaca aatggatatc
     2941 aaaattcaac ccggaaggtt tatctaaaga ggtggacaaa tttactgagc ttaccattga
     3001 agctacgttc atggtacgat catccttttt tctcttgttt ggatttttga ttgaaacaaa
     3061 agaagtaatc gacatccata cccttccatg gagcgtttca attatcgtaa gcattatcct
     3121 aatgagaatt gtgcaactaa agctttctaa actaccgatt atgcctctcc tttttgtagc
     3181 tccgagagga ttaatcacca ttcttctgtt tttatcaatt gatccatcca gcaaaattcc
     3241 gatggtcaat aactcaatgg ttattcaggt tgtgatatta tcggccttac tgatgatgtt
     3301 tggattgatg attcataaaa aagttccgga agaaggggaa caaagtaacg cggaactata
     3361 acgccgttaa ctctcaggtg cgagttccac tttaatgccg tctatttcat ccgacaactt
     3421 aatctgacag cctaagcgcg aattggattt tacatgaaaa gcctgatcaa gcattgccaa
     3481 ttcatcatcg ctttgctccg gtaaagcggt atcgctaatc aggtaacatt gacaagttgc
     3541 gcacattgcc atcccaccac atgtaccttc aaccggcaat tcatacgatt tgcaaagctc
     3601 catcaagttc atccccatgt cggtaggggc ctccaaaacg tgctcttttc cttccctgtc
     3661 tactactgta acagttatcg tgttctctgc catattcaga ttaaaatccg tttacaccat
     3721 ttacggttgt gtacttcaat acaaggtttt tatcaggata aattcgctta aatgcagatt
     3781 gaaccattaa agttccttcg tgaaatccac acagaattag cttcagtttt ccaggatagg
     3841 tatttacatc accaattgca tagataccgg ggatattggt tgaataatca aaggtattta
     3901 ccttgataga ccccttctca atttccagtc cccagttggc aatcggacca agttttggag
     3961 ttaaaccaaa caagggaatg aaatgatcgg ttgccagatc atattcgcct ttatcttttt
     4021 gctgaatcac tacctgttca accgatgaac cgcctttcag accaacaact tcaccatcca
     4081 aaaccagatt aagttttcct tctgcagcca aatcaaaaac gcgctgtaca gaatcaaggt
     4141 gacctctgaa tgtttgtgaa cgatgaacaa gtgtaacgtg agctgcaatc tttttatcga
     4201 tcaggtaaat tgtccaatcc aatgcagaat cgccaccgcc tgcaataacc acacgctttc
     4261 cacggtagaa ctcaggatcc ttaatgatgt attcaacccc tttgtcttcg aagtcagtaa
     4321 tattgggaat tggcggtttt ctgggttcaa aaactcctag tccgccggca attgccacaa
     4381 tcggtgcaat gtgttgagtg cctttgttgg tggtcaccac aaatttgcca tcctctcgtt
     4441 tctcaacatg ttcggctgat tcacctagcg taaaaccggg tttgaaaggt gctgcctgct
     4501 ccatgagatt atccaccaac tcacctgcaa gtacagaagg gaatccggga atatcataaa
     4561 taggcttctt gggatagatc tccgaacatt gcccgccggc ctgaggcaag ctgtcgataa
     4621 ggtgacaacg aagcttcagt aatccggctt caaacacagt gaagagcccg catggaccgg
     4681 ccccaacaat gataatatca gtttcaattg gtgaattcat gggcgcaaat ttacaatttc
     4741 cttagccttc tgagagaaat gaaagtcaca aagtcagtta agatgtttcc aacaagccaa
     4801 acccgagatt gttccggcac aaaagtaagc cgcggctagt aacttgtaaa tgaagcttca
     4861 gggcatttgc cccatacaat tttaaatcct gttcttgtcg atgctctcaa agcgaatgag
     4921 ataccgtgtg ccctgcc
//
LOCUS       DAOMYG010000246         4929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5930_length_4929_cov_7.640952, whole genome shotgun sequence.
ACCESSION   DAOMYG010000246 DAOMYG010000000
VERSION     DAOMYG010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5930_length_4929_cov_7.640952"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    48..147
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(496..1143)
                     /gene="rpe"
                     /locus_tag="PK610_08695"
     CDS             complement(496..1143)
                     /gene="rpe"
                     /locus_tag="PK610_08695"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRJ36053.1"
                     /translation="MKPIIAPSILAADFGNLQRDCEMVNASAAEWFHIDVMDGVFVPN
                     ISYGMPVISAIHTHAKKPLDVHLMIVQPERYIKEFKACGAAILTVHYEASTHLHRTFQ
                     AIKAEGMKAGVALNPHTPVSVLEDIAADADLILIMSVNPGFGGQKFIENTYKKVAQTK
                     ELVLRTNSKAIIEVDGGVTSANAKQLIDAGAQALVAGSFVFSAKDPIATISELRG"
     gene            complement(1146..1652)
                     /locus_tag="PK610_08700"
     CDS             complement(1146..1652)
                     /locus_tag="PK610_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRJ36054.1"
                     /translation="MSNRTIVLTHDQIQQKIRRIAHEIYEHNYQEEKIILIGIAEQGY
                     TLAERLHQLMKEVNGDMKVELGKLTMNKENPLNGSINLSIHPQELNNQTLVLVDDVLN
                     SGKTLIYAAQYLLQSPLKKMNTVCLVDRRHRRFPIRADFVGLTLSTTIQEHIEVEFKS
                     GNDTVYLV"
     gene            complement(1698..2375)
                     /locus_tag="PK610_08705"
     CDS             complement(1698..2375)
                     /locus_tag="PK610_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRJ36055.1"
                     /translation="MIIKVKIEIIRHHKTSQFTGKKLHYFYSMKLDNNYWNERYISAD
                     TGWDIGHVSPPLKMYLNKLASEGKTNLAILIPGCGNGYEAEYAHRMGFTQVFPADYAP
                     EALKKFAERVPDFPKEHLLEGDFFEIKQQFDLILEQTFFCAINPELRDQYVDKCAALL
                     VPGGTLAGLLFNDPLNQDQPPFGGSKDEYLQRFSRKFEILQMDITPYSIEPRAGRELF
                     FELCRKD"
     gene            2368..4248
                     /locus_tag="PK610_08710"
     CDS             2368..4248
                     /locus_tag="PK610_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HRJ36056.1"
                     /translation="MIKIPKKSTPRWIIFSIDMVICLFSLGIAYLIRFDFNSFPVDEE
                     WPVLKVALPFFLIIRAASFYFGKTFVGIIRYTSTEDSKRIFLTVTAGTVIMAVLVPVR
                     YAMDGYFFLPISILVMDYLLTTFLMITFRIAVKLMYAEQVNPSGDKTKVVIYGAGELG
                     LITKRTLDRDGGTRYKVVAYIDDDSKKAGKSLEGVMIHSSAQLEDILERNQIDQLIVG
                     ILHPDAIKKKNIIEICIKHGVSVLNVPPVQKWINGELSFSQIRKVKIEDLLGRPAIQL
                     DEKNIQSALSGKRILVTGAAGSIGSGIVRQLIRFQPSKIILLDQAESALYDLEMELWR
                     NGHLSLCEVVVADITIPERMERVFSHFKPQVVFHAAAYKHVPLMEDNPSEALKTNIEG
                     TRILVDLSLEHAVERFVMISTDKAVNPTNVMGATKRVAEIYAQCAHGNHTRFITTRFG
                     NVLGSNGSVIPLFTKQIEEGGPVTVTDRNVTRYFMTIPEACQLVLEAGVMGQGGEIFV
                     FDMGESVRIYDLAEKMIRLSGLEPERDIQIRITGLRPGEKLYEEVLSDKETTLPTHHP
                     QIMVAKVREYPRKEIVEKVNALVDLFNTQDNMLIVSRLKDIVPEYLSQNSIFQKLDSE
                     KD"
     gene            complement(4487..>4929)
                     /locus_tag="PK610_08715"
     CDS             complement(4487..>4929)
                     /locus_tag="PK610_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36057.1"
                     /translation="FLFFFSSIRPTPSAITIQNHLKEEFDYLKSLKVSGSEEKLVQLL
                     NYAPALKGNMLLVLPKQELKELEEKFDNYIREKSSSGEDGWWYIHIGGNHYSSSTEFY
                     NDASFSGETGSGESSISDWNDSVFSSDSASGCGGCSGCGGCGGD"
BASE COUNT         1333 a          980 c         1043 g         1473 t
ORIGIN      
        1 gtgaccgaca aaagagcgtc agtatcgatg atgtttgcgt gatcggannn nnnnnnnnnn
       61 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      121 nnnnnnnnnn nnnnnnnnnn nnnnnnngtc cgcgtgtaga gaacgggact gggaacgaga
      181 acgaaatttg acattacttc acggttcatt taccgttcac tgattaccgt tcactgatta
      241 ccgttcaccg ttcaccgttc actaattaca gttcgccgtt cactgattac tgttcaccat
      301 tcactgatta ctgttcacca ttcactgatt actgttcact gataactgtt caccaaaaca
      361 tgattgcgtg atcggagtcc gcgtgtagag aacgggactg ggaacgagaa cgaaatttga
      421 cattacttca ccgttcactg attactgttc accattcacc aatacatgtt tgcgtgatcg
      481 gaacatgggt ttgatttatc cccgcaattc tgaaatagtt gcaataggat cttttgcact
      541 gaatacaaaa cttcccgcta ccagtgcctg agcacctgca tcaatcaact gttttgcatt
      601 ggcgcttgtt acccctccat ccacttcaat gattgccttt gagttcgtac gtaacaccag
      661 ttctttggtc tgagctactt ttttataggt attctcaatg aatttttgtc cgccaaaacc
      721 tggattcaca ctcattatca aaataagatc tgcatctgct gcaatgtcct ccaatacact
      781 aaccggagta tgcggattga gtgcaactcc tgctttcatt ccttctgctt taatagcctg
      841 aaatgtgcgg tgtaaatggg tggaagcttc gtaatggacc gttaaaatgg ctgcaccgca
      901 tgctttaaat tccttgatat agcgttcggg ttgtacaatc atcaaatgga catccagcgg
      961 ttttttagca tgtgtgtgaa tggcagaaat taccggcatt ccgtaactta tatttggaac
     1021 aaaaacgcca tccattacat caatatgaaa ccattctgcg gcgcttgcat ttaccatttc
     1081 gcaatcgcgc tggaggttgc caaaatctgc tgcaagtatt gagggtgcaa taataggttt
     1141 catgttcaaa caagatatac ggtatcgtta ccggatttaa attctacttc tatgtgttcc
     1201 tgtatagttg tgcttagtgt tagcccgaca aaatccgcac gaatagggaa acggcggtga
     1261 cgacggtcga caaggcaaac agtattcatt ttttttaagg gcgactgcaa aagatactgc
     1321 gccgcataga tcagtgtttt tccgctgttc agtacatcat ccaccaaaac aagtgtttga
     1381 ttgttcagtt cttgtggatg aatgctgagg ttgatggagc cattaagcgg gttttccttg
     1441 ttcatggtca gttttcccaa ttcaaccttc atgtcaccgt taacttcctt catgagttga
     1501 tgcaaacgtt cggcaagcgt atatccttgt tctgcaatac caatgagaat gattttttct
     1561 tcctgatagt tgtgctcgta gatctcgtga gcaattcggc ggatcttctg ttggatctgg
     1621 tcgtgggtaa ggacgatcgt tcggttcgac attcaggcgg ttttgaaggt acaaaatacg
     1681 ggaaaaaaag aataagatca gtctttgcga caaagctcaa aaaacaattc ccttcctgct
     1741 ctgggttcga tgctgtacgg ggtaatatcc atttgtaaaa tctcaaactt acggctgaaa
     1801 cgctgcaggt attcgtcttt ggagccacca aagggcggtt ggtcctgatt aagtgggtca
     1861 ttaaaaagca atcctgccaa tgtaccacca gggactaaga gtgccgcaca tttatccacg
     1921 tattgatcac gaagttcggg gttgatggcg cagaaaaatg tttgttccag aatcagatca
     1981 aattgttgct tgatttcaaa gaaatcacct tccagtaaat gttccttcgg gaaatccggt
     2041 acacgttcgg caaatttttt cagtgcttca ggggcataat cagcaggaaa tacctgtgtg
     2101 aaacccatac ggtgtgcata ttctgcttca tacccattgc cgcatcccgg aatgagaatg
     2161 gcaagattcg tttttccctc ggaggcgagt ttgttgagat acatttttag gggaggagaa
     2221 acgtgaccga tatcccatcc ggtatcggca ctgatataac gttcgttcca ataattgttg
     2281 tcgagtttca tgctgtaaaa ataatgtaat ttttttccgg taaactgaga ggttttgtgg
     2341 tgacgaataa tctctatctt taccttgatg atcaaaatcc ccaagaaaag caccccgcgc
     2401 tggattattt tctccattga tatggtgatt tgccttttct ctttggggat tgcctacctt
     2461 attcgtttcg atttcaattc ctttccagtc gatgaagaat ggccggttct taaagtggca
     2521 ttgccgtttt tcctgattat cagagctgcc tctttttatt tcggaaagac ttttgttgga
     2581 ataatcagat acacaagtac tgaagattcc aaacggattt ttcttacagt tactgccggt
     2641 accgttatta tggctgttct ggttccggta cgttatgcaa tggatggtta ttttttcctt
     2701 ccgatttcca ttcttgtaat ggattatttg ctgactactt tcctcatgat cacctttcgc
     2761 attgcagtga aactgatgta tgccgaacag gtgaatccat ctggcgataa aaccaaagtg
     2821 gttatttacg gtgcaggaga attgggattg atcaccaagc gtacactcga tcgcgacggt
     2881 ggaacacggt ataaagtagt tgcctatatc gatgatgata gtaaaaaggc aggcaaatcg
     2941 ctggaggggg ttatgattca ttcctctgca caacttgaag atattcttga gcgtaatcaa
     3001 atcgaccagc tgattgtcgg aattcttcat ccggacgcaa ttaagaaaaa gaatatcatc
     3061 gaaatttgta tcaaacatgg tgtttcggtg ctgaatgttc ctccggtgca gaagtggatc
     3121 aacggagaat tgagtttttc ccagatcaga aaagtgaaaa ttgaagattt acttggacgc
     3181 cctgcaatac agctcgatga gaaaaatatt caatctgctc taagcggaaa acggatactg
     3241 gtgaccggtg ctgctggttc aattggaagt ggaattgttc ggcaattgat acggtttcag
     3301 ccctcaaaaa taatcttact cgatcaggcc gaatctgcat tgtatgatct tgaaatggag
     3361 ttgtggcgta atggacattt gtccttatgc gaagtcgtgg ttgcagatat taccattccc
     3421 gaacgaatgg aacgtgtctt ttctcatttt aagcctcagg ttgttttcca tgcagccgcc
     3481 tataagcatg tcccgttaat ggaagataat ccttcagaag cgctgaaaac gaatatagaa
     3541 ggcacacgta ttctggttga tctttcgctg gaacatgcgg ttgaacgttt tgtgatgatc
     3601 tcaacggata aggcggtaaa tcctaccaat gtaatgggtg ctacaaaacg ggttgcagaa
     3661 atttatgcgc aatgtgctca cggcaatcat acacgtttta tcaccacgcg ttttggaaat
     3721 gtattgggtt caaacggttc ggtaattccg cttttcacaa aacagataga ggagggcggg
     3781 cctgttactg ttacagatcg taatgtaaca cggtatttta tgactatccc tgaagcttgt
     3841 cagctggtac tcgaagccgg agtaatgggg caggggggag agatctttgt atttgacatg
     3901 ggtgagtctg tacgaattta tgatctggca gaaaaaatga ttcgattatc cggactcgaa
     3961 cctgagcggg atattcaaat tagaataaca ggattgcgtc caggtgaaaa attgtacgaa
     4021 gaagtattga gcgataagga aactacgctc ccaacgcatc atcctcaaat catggttgcc
     4081 aaagtcagag agtatccgcg aaaagagatt gttgagaaag tgaatgccct tgtagatctt
     4141 ttcaatactc aggataacat gctcatcgta tcgcgattga aagacatagt tccggaatac
     4201 ctttcgcaaa actctatctt tcaaaaactg gatagtgaaa aggattaaga tcttaaagta
     4261 atccgatttg tatgctgcta atctctctat cgaaattttc ctttcagaaa gttaaaacta
     4321 tttaatagcg tttaaatcgt tcgatgattc atttcgctta gtgagcttcc tttttaatca
     4381 gttatcagtt atcagtaatc tgtaattagg taattggtaa ttaggtaatc ggaaattggt
     4441 aattaggcaa tcgaaaattg gtaattaggc aatcggaaat aaaaacttaa tcacctccac
     4501 atcctccaca accgctgcaa ccaccgcatc ctgatgcact gtcggatgaa aaaacactgt
     4561 cgttccaatc tgaaatagaa ctttcaccgg aacctgtttc tccactgaat gaagcgtcat
     4621 tataaaattc agttgagctg ctgtagtggt taccaccgat atggatatac caccatccat
     4681 cttcacccga cgaagacttt tcccttatgt agttgtcgaa tttctcttcc aactctttca
     4741 attcctgttt gggtaatacc aataacatgt ttcctttaag tgcaggggca taattcagca
     4801 attggaccaa tttctcttcg ctccctgaaa cttttaatga tttcaggtaa tcgaattctt
     4861 cttttaaatg attttgaatg gtaatagctg aaggtgttgg tctgattgaa ctaaagaaaa
     4921 ataaaaatc
//
LOCUS       DAOMYG010000247         4915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_5964_length_4915_cov_8.232716, whole genome shotgun sequence.
ACCESSION   DAOMYG010000247 DAOMYG010000000
VERSION     DAOMYG010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5964_length_4915_cov_8.232716"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PK610_08720"
     CDS             complement(<1..506)
                     /locus_tag="PK610_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36058.1"
                     /translation="MRNGITLFILLVYTLSAGAQIGDATINSEGKRISFISGMLFNNK
                     DFFFDFGLGYENLTAQWAIRLDVDFRPFFKKVQIKNEDGSIDQFFERKTLLGIAAEKR
                     LLQLPVGSSTFRFLVGAKGGVMFGNYRGLRRHAEILPYVSPYAGAAFDIGSGGMFKFG
                     YSFMDLQNP"
     gene            653..2842
                     /locus_tag="PK610_08725"
     CDS             653..2842
                     /locus_tag="PK610_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRJ36059.1"
                     /translation="MRTFLFTLFLLPGILQAQKQMLSNEIIWASPALYAESVDHVRSM
                     NDGEHFTAIDNENGVGVVNKYSFKDYKKVGRIVSSVDIKHDGEAVMIDDYEFNADETM
                     LLIASGIEPIYRRSYTANYFIYDIRKKTTTPLGDLAKGKQMLAEFSPDGKKVAFTRNN
                     DIFIYDLASGKETQVTNDGQRNAIINGSTDWVYEEEFSIVKAFYWSPNSTKIAYYRFD
                     ESKVKEFTMTMYGELYPDLYTFKYPKAGEDNSLISLFVYSVNGGQKASIDLGPETDIY
                     IPRINWTNNDDVLCVQRMNRLQNKLEYCLVKAGNGSTGILPMTPMYTESSSTYVEIND
                     DLIFLKGKDAFIRTSEKDGYNHIYLIGFDGKSTQITSGKWDVVEFKGLDEKNGLIYYI
                     SAESAPYQKDLYVVKLDGSGKKKLSTRPGVNDAHFSNGMKYYINYHSDANTPYYITLH
                     NSTGKELKVLMDNKEVMNRMKQYDLGKKEFFKFTTSENVEIDGSIIKPSNFDPQKKYP
                     VYLFVYGGPGNNEVMDSWDGPNYFWHQMLAQKGYLVVCVDPRGTMFKGEQFKKCTYKQ
                     LGKLETQDLIETAKWLGRQSWVDKSRIGVQGWSYGGYMTLLAMTKGAEYFKTGISVAP
                     VTNWRYYDNIYTERFMQRPQENASGYDDNSPINHVKKLKGKLLLVHGSGDDNVHYQNT
                     MEMINALVAANKQFELFIYPNRNHGIYGGYTRLHLFTMMTNFVEKNL"
     gene            complement(2948..4339)
                     /gene="glmM"
                     /locus_tag="PK610_08730"
     CDS             complement(2948..4339)
                     /gene="glmM"
                     /locus_tag="PK610_08730"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRJ36060.1"
                     /translation="MTLIKSISGIRGTIGGHSGDGLTPLDTVKFAAAYGQWLISRNPR
                     MKVKVVLGRDARISGEMVRSLVSATLQGMGIDVIDLGLSTTPTVEVAVPGEKAHGGII
                     LTASHNPRQWNALKLLNEKGEFISGADGAEVLRIAEAEQFYFAEVDDLGVFSTSDTWI
                     KKHIDLVLRLPYVDKDAIAKANLHVVVDAVNSTGGIAVPALLEALGVNSITKLYCEPN
                     GDFPHNPEPLPEHLTEISSLIKKEKADVGIVVDPDVDRLALVCENGEMFGEEYTLVAC
                     ADLVLQSKKGNTVSNLSSTRALRDVTEKYGATYAASAVGEVNVVDLMKSTNAVIGGEG
                     NGGVILPELHYGRDALAGIALFLTLLAKSGKSCSQLRASYPDYFISKNKIELTPQIDV
                     DKVLSAMKAKYASQPVNDIDGIKIEFGNEWVHLRKSNTEPIIRIYSESKDKASADALA
                     QRIIVDIRGIAGI"
     gene            4507..>4915
                     /locus_tag="PK610_08735"
     CDS             4507..>4915
                     /locus_tag="PK610_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36061.1"
                     /translation="MSSNAFTWSWLNPLPQGNRLNDVQSFNGSDTLFAVRNTGTIMSS
                     ANANLGFAVGEKGTILKAVRNPPLVESIGEISGIEPISVFPNPCSEYAIVEGRALKSG
                     SAEFFLYDLNGKLIYSRTLFSERFTLPTQNLPAG"
BASE COUNT         1499 a         1064 c         1039 g         1313 t
ORIGIN      
        1 gggttttgca aatccataaa agaatagccg aacttaaaca tgccacccga accaatatcg
       61 aaagcagcac cagcataagg tgaaacataa ggaagaattt cggcgtggcg acgaagcccg
      121 cggtaattcc cgaacataac gccacccttt gcgcctacta aaaaccgaaa agtggacgaa
      181 ccaaccggaa gttgcagcaa tcgtttttcg gcagcaattc ccaacaaggt tttccgctcg
      241 aaaaactgat cgatactgcc atcctcattc ttgatctgca cctttttgaa gaagggtctg
      301 aaatccacat caagtcgaat cgcccactga gcagtaaggt tttcataacc cagtccaaaa
      361 tcaaaaaaga aatccttgtt attgaagagc atgcccgaga tgaaggagat ccttttgcct
      421 tcggaattga tcgttgcatc accgatttga gcacctgcag acaaggtgta aaccaggagt
      481 ataaaaagcg ttataccatt tctcacggct caaaaatagg gatttcacct caattccatt
      541 catcaggatg gctgtcctcc tgctccattc ttcaggtata tatgccattc cccatttttg
      601 gaccgaaaat ggattgattt ttggtatttt cgtcctttac acaaatcaca ctatgagaac
      661 tttcctcttc accctatttc ttttacccgg aatactgcag gcacaaaagc aaatgctcag
      721 caacgaaatc atctgggcga gtccggcatt gtatgctgag agtgtcgatc acgtaagatc
      781 catgaacgac ggcgaacatt ttacggccat cgataatgag aacggtgtgg gtgttgtgaa
      841 caaatacagt tttaaagatt ataaaaaagt aggtcgtatc gtttcttcgg tcgatatcaa
      901 acacgatggc gaagcggtga tgattgacga ttacgaattc aatgccgacg aaaccatgtt
      961 gctcattgct tcgggcatcg aaccgatcta ccgcagatcg tacacagcca attatttcat
     1021 ttacgatatc cggaaaaaaa caacaactcc gcttggagat cttgcaaaag gcaaacaaat
     1081 gctcgctgaa ttttctccgg atggaaaaaa ggttgccttt acacgtaaca atgatatttt
     1141 catttacgat ctggccagcg gaaaagaaac gcaggttacc aatgatggtc agcgcaacgc
     1201 tatcattaac ggtagcaccg attgggtgta tgaagaagaa ttttccattg taaaagcttt
     1261 ttactggtcg cccaactcca ccaaaattgc gtattaccgt ttcgacgaaa gcaaggtaaa
     1321 ggaatttacc atgaccatgt acggagaact ttatccggac ctctacactt ttaaataccc
     1381 caaggcagga gaagataatt cactgatcag tctgttcgtt tattcagtta acggcggaca
     1441 aaaagcaagt attgatttgg gtcctgaaac ggatatatat atcccccgca tcaactggac
     1501 caacaacgat gatgtgttgt gtgtgcaacg catgaaccgc ctgcagaaca aactggaata
     1561 ctgtctggta aaagccggca acggaagcac cggtattttg ccgatgacac cgatgtacac
     1621 cgaaagttca tccacttatg ttgagatcaa tgatgatctg atctttttaa aaggaaaaga
     1681 tgcctttatc cgcacctcgg aaaaagacgg ttacaatcat atttacctga ttggtttcga
     1741 tggaaaatca acacagatca ccagcggaaa atgggacgtg gtggagttta aggggctcga
     1801 cgaaaaaaac ggactcattt attacatctc tgcagaatca gctccctatc aaaaagattt
     1861 gtacgtggta aaattggatg gaagcggaaa gaaaaaacta tctacacgtc cgggtgttaa
     1921 cgatgcacat ttcagcaatg ggatgaagta ttacatcaat taccattctg atgccaatac
     1981 accgtattac atcactttgc acaacagtac cggtaaggaa ttgaaagtgc taatggacaa
     2041 taaggaagtg atgaacagga tgaaacaata tgatttaggg aagaaagaat ttttcaaatt
     2101 taccacttca gaaaatgttg agatcgacgg ttccattatc aaaccttcca attttgatcc
     2161 gcaaaaaaaa taccccgttt atttgtttgt atatggaggt ccgggcaaca atgaagtgat
     2221 ggattcatgg gatggtccta attatttctg gcaccagatg cttgcccaga aaggatatct
     2281 tgtagtatgt gttgatccac gcggaaccat gttcaagggc gaacaattta aaaaatgcac
     2341 ctacaaacaa ctcggaaaac tggaaacgca agacctcatt gaaacggcaa aatggctcgg
     2401 acgacaaagt tgggttgata aatcgcgtat aggagttcag ggctggagtt acgggggata
     2461 tatgacctta ttggcaatga ccaaaggagc agaatatttc aaaaccggaa tctctgttgc
     2521 gccggttacc aactggaggt attacgataa tatctacacc gaacgcttca tgcaacgtcc
     2581 gcaggaaaac gcatccggtt acgatgataa ttcaccgatc aatcatgtaa agaaattaaa
     2641 aggaaaactt ttattggtgc atggtagcgg agatgacaat gtgcattacc agaatacaat
     2701 ggaaatgatc aacgcattgg ttgcggccaa caaacaattt gagctgttta tttaccccaa
     2761 tagaaatcac ggaatttatg gtggctacac gcgtttgcat ctctttacga tgatgacaaa
     2821 ctttgtggag aaaaatctgt gaggatggta ttggtgaagg atgaacagtg aagggtgaac
     2881 agtgatcccg acgctatcgg tcgggacaag tcggtgaacg gtgattggta attggaaatc
     2941 ggtataatca aatccctgct atcccgcgaa tatctacaat gatgcgttga gcgagtgcgt
     3001 cggcggaggc tttgtctttt gattcggaat agatacggat aatgggttcc gtgtttgatt
     3061 tacgcaagtg aacccactcg ttaccgaatt caatttttat gccgtcgata tcgtttaccg
     3121 gttgcgacgc gtatttcgct ttcatggcag ataaaacctt atcgacatca atttgcggag
     3181 tgagttcgat tttattcttt gaaatgaaat aatcaggata agaagctcga agttgcgagc
     3241 aggattttcc acttttcgca agtaatgtca ggaacaaagc gatacctgca agtgcatcac
     3301 gaccataatg caattccggc agaataacac caccattacc ctcgccaccg atcactgcgt
     3361 ttgttgattt cattaaatcc accacgttaa cttcaccaac agccgatgct gcatacgttg
     3421 ccccgtattt ctcggtgaca tcccgcaaag cccgtgttga agacagattg gaaactgtat
     3481 tacctttttt tgattgcaat accagatcgg cacaggcaac cagtgtatat tcttcaccga
     3541 acatctctcc gttttcgcaa accaaagcaa ggcggtcgac atcaggatct accacaattc
     3601 ctacatccgc tttttctttt ttgataagtg aagaaatttc agtgaggtgt tcaggtaatg
     3661 gttccggatt gtgagggaaa tcgccattcg gttcgcaata caattttgta atgctgttca
     3721 caccgagcgc ttcaagcaag gcaggaacag caattcctcc tgttgaattt accgcatcaa
     3781 caacaacgtg caaatttgct tttgcaattg catctttatc gacatagggt aatcgaagaa
     3841 ccaaatcaat atgttttttg atccaggtat cactggtaga aaagacaccc agatcatcca
     3901 cttcagcaaa ataaaattgt tcagcttctg ctatgcgtaa tacttctgca ccgtcagcac
     3961 ccgaaataaa ttctcctttt tcattgagca atttaagtgc attccattga cgcggattat
     4021 gtgaagcagt gagaatgatt cctccatgcg ctttctcacc cggaacagct acttcaacag
     4081 tgggtgtagt ggataatcct aaatcaatga catcaattcc cattccctgc aaggttgctg
     4141 agaccagcga gcgaaccatc tcaccggaaa ttcgtgcatc tcttcccaat acaactttca
     4201 ctttcatgcg cggattgcgg ctgatcagcc attgtccgta tgcagcagca aattttaccg
     4261 tatcaagcgg tgttagtccg tcgcctgaat gaccaccgat tgttccgcga attcctgaaa
     4321 tggatttgat gagagtcata actaaaaatt acaagtgcta aagtaatctt atttaaaacc
     4381 atgactgcga acaagtaaaa aaaaagtgct gcccgaacga aatctgttta ctttcatgca
     4441 tataaaaccc tgaacacgtc acgatttttc tttgctcttt tcactatttt ttgcttcatt
     4501 ctccctatga gcagcaatgc ttttacctgg tcgtggctaa atccattacc acagggtaac
     4561 cgactaaacg atgttcaatc ctttaacgga tcggacacac tttttgcggt gagaaatacc
     4621 ggcaccatca tgagttcggc aaatgcaaat cttggttttg ccgttggtga aaagggaaca
     4681 attcttaagg ctgttcgaaa tccgccattg gtagaaagta ttggagagat aagtggaatt
     4741 gaaccgattt ccgtttttcc gaatccatgc agcgaatatg caattgtgga aggcagagct
     4801 ttgaaaagtg ggagtgctga atttttcctt tacgatttga atggaaaact catttacagc
     4861 cgcacacttt tttcggagag atttactcta ccaacacaaa acctaccggc cggca
//
LOCUS       DAOMYG010000248         4850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6079_length_4850_cov_7.104901, whole genome shotgun sequence.
ACCESSION   DAOMYG010000248 DAOMYG010000000
VERSION     DAOMYG010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6079_length_4850_cov_7.104901"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PK610_08740"
     CDS             complement(<1..351)
                     /locus_tag="PK610_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ36062.1"
                     /translation="MGLFGRNYTNRTDEELMAAIVDGDKRAFEELYSRYAKPMVNFFC
                     RMLWKDREKAQDFMQDLFTKIVHKPESYDASRPFKTWIYSVANNMCKNEYRKQEVRIA
                     PGQFAGQVRIDEPEM"
     gene            complement(365..1576)
                     /locus_tag="PK610_08745"
     CDS             complement(365..1576)
                     /locus_tag="PK610_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36063.1"
                     /translation="MKYIFVSVLLALGGIMPAMGQTNTGGGQQPPQEEKNKIQSQSTN
                     MNENLANPVILEPSRSAQSPGFYRGQTETLRRAVDFNKYDAKSWLGYYKAERYSYYTK
                     TSNDINTEQQSELDKIVDEMSQYVPTSYEFNYAKYLNGNHDIRLFGFLAKAYEINPKA
                     TELFDQFVSYYELTGDAVRKTDWCKKLNESGEIPKEIMEFAYNLLVSLDENAVIITHG
                     EMDSHSSFIWQTVKNHRKDVLILYMDLLEKPEYREKKFRELGINVTASPYTNKAAFVA
                     EFLQKNSLKRPSYLATTVAPDLIRPIQSKLFLTGLAFKYSEKGFDNLPVIAENWEKKF
                     RKEELAKKIAQGSVSAKINMNYVPGLLLLRDYLLSKGLNDKAKATEELALKIGSEGGK
                     EQQVRTMIMKK"
     gene            1682..2242
                     /locus_tag="PK610_08750"
     CDS             1682..2242
                     /locus_tag="PK610_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRJ36064.1"
                     /translation="MEYNYYKILGVHQKASDAELKVAFREMAKKFHPDRNKAPDSHTF
                     FQVLNEAYQTLSDPVRRFSYDQKLGYKTILEEPQAAAKRGSREWRMERRKRNAEYSHS
                     IHKAPEMWEPHVIVKFFFYCVGLAFGSFTMYYSGDYILNGLWSPVMLWVPLLGFLIFL
                     DALSGMFTGQPVLTVKLFSRLKQRFS"
     gene            2312..2749
                     /gene="mscL"
                     /locus_tag="PK610_08755"
     CDS             2312..2749
                     /gene="mscL"
                     /locus_tag="PK610_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRJ36065.1"
                     /translation="MGLMKEFREFAMRGNVVDLAVGVIIGGAFGKIVSSLVSDIIMPP
                     IGMLMKGQNFADLFMSLDGNTYESIAKAKEAGAPVLAYGSFLQTVLDFIIVAFAIFMM
                     IKGMNRLNKLKKKEEEAQAAAAAEPEPTKEQVLLTEIRDLLKK"
     gene            2765..4003
                     /locus_tag="PK610_08760"
     CDS             2765..4003
                     /locus_tag="PK610_08760"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRJ36066.1"
                     /translation="MRREDLKVFELKNGIQAVFEPEDHSEVVHAVLTIGAGTRDEYPE
                     EQGMAHFIEHCLFKGTKKRNGIEVISQLDSVGGELNAYTTKEETCIYASFHKHFLERG
                     LELIADIVFHSVFPPAEMRKEKTVIIDEIRSYLDSPSELIFDEFEEQVYKGHPLGHNI
                     LGTEKTLKRFDKAMVKEFMQRNYVPSRMTLSISGKLPEAKAKFLAEKYFGHARSSDGF
                     NSRVLPVPAKPFKLKKNYSTFQDHVVWGGTAYRYEDKKRTAFVLLNNVLGGNAMNSRL
                     NLVLREKHGFAYNVEASYVPYTDTGLFTIYFGTDKRNRAKCEELVLKELQNFRKKKFT
                     SSQLEQAKIQLKGQLALSEENRLNRALGLGKSLLLYGKIQKMRDVYRRIEKVSAEEMQ
                     DVANELYNPDQLSRLVYTNK"
     gene            4003..4644
                     /locus_tag="PK610_08765"
     CDS             4003..4644
                     /locus_tag="PK610_08765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HRJ36067.1"
                     /translation="MDFIAPELDQYACLHSDEESELLKRINRETHLKVLTPRMLSGHL
                     QGRVLAMLSHMIKPRRILEIGTYTGYSALCIAEGMTADGKIITIDINAELEERVRGYF
                     AESPYSGKIDFRVGNAVDLIPGIEEKFDLVFIDADKINYPAYFDLVMDKLNTGGFIIA
                     DNVLWSGKVLMEEELMDKDTKAIHEFNSKVQNDSRVENVLFPIRDGLMIVRKK"
     gene            complement(4645..>4850)
                     /locus_tag="PK610_08770"
     CDS             complement(4645..>4850)
                     /locus_tag="PK610_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (uracil-5-)-methyltransferase RumA"
                     /protein_id="HRJ36068.1"
                     /translation="RAGMHEDVIQTILKAAPEKIVYVSCNPATQARDLALMKDNYSIE
                     VVQPVDMFPQTHHVENVVLLVRK"
BASE COUNT         1331 a          964 c         1063 g         1492 t
ORIGIN      
        1 catctccggc tcgtcgatcc ggacctggcc agcgaactga cccggagcga tgcgcacttc
       61 ttgttttcgg tattcgtttt tacacatgtt attggctaca gaataaatcc aggttttgaa
      121 cggacgggat gcgtcgtacg actcaggttt gtgcacgatc ttggtaaaca gatcctgcat
      181 aaaatcctgt gccttttcac gatccttcca gagcatccgg cagaaaaagt tgaccatcgg
      241 ttttgcatac ctcgagtaca attcctcaaa agcacgctta tcgccgtcaa cgattgcagc
      301 catcagttcc tcatcggtcc tgttcgtata gttccttccg aaaagcccca tccgaccggt
      361 tctgctattt tttcattatc atagttcgta cttgttgttc ttttcctcct tccgagccga
      421 tttttaaagc cagttcttcc gttgccttgg ccttatcatt caaacccttg ctcagcaaat
      481 aatctctaag cagaagtagt ccgggtacat aattcatgtt gatttttgca cttaccgaac
      541 cctgtgcgat ttttttggca agctcttcct ttctgaattt tttctcccag ttttcagcaa
      601 tcacagggag attatcaaaa cctttttcgc tgtatttgaa tgccagtcct gtgaggaata
      661 atttcgattg tatcgggcgg attaaatcag gtgccactgt tgttgccaaa tagcttggac
      721 gcttcaacga atttttttgg aggaactctg caacgaaagc cgctttgttg gtgtaaggac
      781 tagcggttac attaattcca agctccctga atttcttttc acggtattcc ggtttttcaa
      841 gaagatccat gtaaagtatg agtacatctt ttcggtgatt ttttaccgtt tgccagataa
      901 aggatgaatg agaatccatt tcaccatgag taatgatcac agcattttca tcaagcgata
      961 caagaagatt gtatgcaaac tccatgatct ctttcggaat ttctccggat tcatttaatt
     1021 ttttacacca atctgttttc cgaacagcat cacccgtcag ttcataatac gagacaaact
     1081 gatcaaacag ctctgtcgct tttggattga tctcataagc ttttgccaaa aatccaaata
     1141 agcgtatatc gtgattcccg ttcaggtatt tggcataatt gaattcatag ctggtaggta
     1201 catattgcga catctcatcc acaatcttgt cgagttcact ttgttgttct gtgttaatgt
     1261 cattgctggt tttggtgtag tatgaatacc gttctgcctt gtaatagcca agccatgatt
     1321 ttgcatcgta cttgttaaag tcaacagctc tgcgaagggt ttcggtctga cctctgtaaa
     1381 atcccggact ctgcgcagaa cgtgatggct ccagaataac tgggtttgcc agattttcgt
     1441 tcatattggt gctttgcgac tggatcttgt tcttttcctc ctgaggcggt tgttgtccac
     1501 ccccggtatt ggtctgcccc atcgccggca taatgccacc aagggcaaga agtaccgaaa
     1561 caaatatgta tttcatgggt ttcatttgat agctgtacac ccttttaaag taaaggttca
     1621 atgtagcgat ttttattctg ttactttttt gaatgttctg aattccttaa ctttatcgta
     1681 gatggagtat aattactaca aaattcttgg agtccaccag aaggccagtg atgcggagct
     1741 gaaggtcgcc tttcgcgaaa tggccaaaaa attccatccc gatagaaaca aagcccctga
     1801 ttctcacacc ttttttcagg ttttgaatga ggcctaccaa acactttctg atcctgtcag
     1861 gaggttttca tacgaccaga aattgggcta caaaactatt cttgaagagc cccaggcggc
     1921 tgcaaaaaga gggagccggg aatggcgaat ggaacgcaga aaacgaaacg cagaatattc
     1981 tcattccatt cacaaagcac ctgaaatgtg ggagcctcat gttattgtta agtttttctt
     2041 ttattgtgtt ggactggcat ttggatcgtt taccatgtac tattccggtg attatattct
     2101 gaatggactc tggagtccgg tgatgctttg ggttcctctt ttgggttttt tgatctttct
     2161 tgatgccctc agcggaatgt ttaccggtca acctgttttg acggttaaat tgttttcccg
     2221 tcttaaacaa cgcttctctt aaccattgat ggtctatttt cctatttttg aggaagtttc
     2281 tgaaaatttc aattaataaa tcaattctcc tatgggatta atgaaagaat tccgcgaatt
     2341 cgcgatgcgt ggcaatgttg ttgaccttgc tgtcggtgta attattggtg gtgcattcgg
     2401 taaaattgta agttcgcttg tatccgatat catcatgccg ccaattggta tgctgatgaa
     2461 aggacaaaac tttgctgatc tttttatgtc gctcgatggc aatacctatg agtcgatcgc
     2521 taaggccaag gaagcaggag cgccggtatt ggcatatggt tcctttttac aaactgtgct
     2581 tgattttatc attgttgcat ttgccatttt tatgatgata aaaggaatga atcgtcttaa
     2641 taaactgaag aaaaaggaag aagaagcgca agcagcagca gcagccgaac cggaacctac
     2701 caaggagcag gttttgctta ctgagattcg cgatttgctg aaaaaataat tatacatcga
     2761 aactgtgcgg agagaagacc tcaaagtatt tgaattgaaa aatggtattc aagccgtatt
     2821 cgagcctgaa gatcattcgg aagtggtgca tgccgtactc actattggtg caggtacccg
     2881 tgatgaatat ccggaagaac agggaatggc gcattttatt gagcattgcc tgtttaaagg
     2941 gacaaaaaaa cgaaatggga ttgaggtcat ctctcaactg gattctgttg gaggcgaatt
     3001 gaatgcttac acaaccaagg aggaaacgtg tatttatgcg tcctttcaca agcattttct
     3061 ggagcgggga ttggaactaa ttgctgatat cgtttttcat tctgtatttc cacctgcaga
     3121 aatgcgcaag gaaaaaacag tgatcatcga tgagatccgt tcttatctcg attctccctc
     3181 ggagttgatc tttgatgaat tcgaagagca ggtgtataag gggcatcctc tgggacataa
     3241 cattcttggt acagaaaaaa ccttgaagcg ttttgacaaa gcgatggtca aggaatttat
     3301 gcagcggaat tatgtacctt ccagaatgac gctcagtatt tccgggaaat tacccgaagc
     3361 gaaggcaaaa tttcttgccg aaaaatattt tggtcatgct cgttcatctg atgggttcaa
     3421 ttcaagggtt cttccagtac cggcaaaacc cttcaaattg aaaaagaact attccacctt
     3481 tcaggatcat gttgtttggg gtggaacagc ataccgttac gaagacaaaa agcgtactgc
     3541 gtttgtattg ctcaataatg tattgggtgg aaacgcaatg aacagcagat tgaatctggt
     3601 gctaagggaa aaacacggtt ttgcatacaa tgtggaagcc agttatgttc cgtatacgga
     3661 taccggattg tttacgatct atttcggtac ggataaacgc aacagagcaa aatgtgagga
     3721 attggtctta aaggaactgc aaaatttccg caagaaaaag ttcacctcat ctcaattgga
     3781 gcaagctaaa attcagttaa agggtcaact tgcactttcc gaagagaacc gtttaaatcg
     3841 tgctcttggt ctgggtaaaa gcctattgct ttacggtaag attcagaaga tgcgtgatgt
     3901 ttaccgtcgc atagaaaagg taagtgcaga agagatgcag gatgttgcca atgagttata
     3961 caatcctgat caattaagtc gattagttta taccaacaaa taatggattt cattgcgcct
     4021 gaacttgatc agtatgcctg cttacattcc gatgaagaat ctgaattgct aaagcggatc
     4081 aatcgcgaaa cacatcttaa agtcttaacc cctcgtatgc tttccggtca cctgcaggga
     4141 agggttcttg cgatgcttag tcatatgatc aagccacgaa gaatacttga gatcggtacg
     4201 tatacaggct attcggcatt gtgtattgca gagggaatga cagctgatgg aaaaataata
     4261 accattgata tcaacgcaga acttgaggaa cgtgttcgtg gatattttgc agaatcaccg
     4321 tattccggaa aaattgattt cagagttggt aatgcagttg atttaattcc tggaatagag
     4381 gagaagtttg atcttgtatt tattgatgca gataagatca attatcctgc ctattttgat
     4441 ttggtaatgg ataagctaaa caccggcgga tttataattg ccgataatgt attgtggagt
     4501 ggaaaggttt tgatggagga agaattaatg gataaagaca caaaagccat tcatgaattc
     4561 aattccaaag tgcaaaatga ttcccgtgta gaaaatgtac ttttccctat tcgcgatggg
     4621 ttgatgattg tgcggaaaaa atagttattt cctcaccagc aatactacat tttccacatg
     4681 gtgcgtttgc ggaaacatat caaccggctg cacaacttca atggaataat tgtctttcat
     4741 aagtgccaga tcgcgtgcct gtgtggcagg attgcaggat acgtacacga tcttttcggg
     4801 agctgctttc agtatggttt gaatgacatc ttcatgcatt cctgctcttg
//
LOCUS       DAOMYG010000249         4849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6082_length_4849_cov_8.039007, whole genome shotgun sequence.
ACCESSION   DAOMYG010000249 DAOMYG010000000
VERSION     DAOMYG010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6082_length_4849_cov_8.039007"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="PK610_08775"
     CDS             complement(<1..1045)
                     /locus_tag="PK610_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36069.1"
                     /translation="MYKITVDGKTVVGCNHDTWYTTPKIWFATAKNSNEYGAAFTGAR
                     PTGANKTAPQSGMNEKGLAFSRLAAYHPKQQNPFPKRLKVGNEVDYLEDILHKCATAK
                     EVRNYIEKYDHSVFHDHVFIYIDTNGSYLIVEPYMLIEGNDSNYVLSNFCPSITDPVK
                     ARQLERYRNGEDYLKTQDVTPSLAYFTALSDTMHVCRKRNGDGTLLTSIWDTKEGSVN
                     LFFYHNYDSTVQFNLSEELAKGDHIIDVSTLFPANPEFERLKSYITPFNTPVLRILLV
                     VIGGLLFFFIVLFTFAAIKLKNTNITYKHTFLIIGINLFLIGYLFVLVTNENIYYFDA
                     PYKHYASALISLSS"
     gene            complement(1170..2306)
                     /locus_tag="PK610_08780"
     CDS             complement(1170..2306)
                     /locus_tag="PK610_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRJ36070.1"
                     /translation="MYSLNELQIEKIHNVIRIHGVVTEDLQLNLLDHICCILEEEYNE
                     NENFDTYLSEVLKRFYKTELREIEEETQLLLTFKHYYAMKKFMFFSGITSTLGILVGA
                     LLKFLHLPGAGILLVLGIAIFTVVFLPIMFTLKFRESKHRRERSILMLAIPMCLLIVT
                     GVLFKVMHWPGANVMTQGSMFFTIFVFIPIYLITGLRNADSKVNTLTTSILMLAGAGL
                     TMSMLTVMKSKAVIQKEIRTIERSDYGIHQRTDFSRTQLEGLKIESEDREQFLNETLK
                     MVDRIETEKQQLLANSGSNELNDETKKSLPYYREVYPNIEKDVRNYQKRFSGLLAGKG
                     IPELEIDLSISPGSTMLNAYQNLCDLQRDLLDYFQFVVIADELK"
     gene            complement(2299..2643)
                     /locus_tag="PK610_08785"
     CDS             complement(2299..2643)
                     /locus_tag="PK610_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRJ36071.1"
                     /translation="MYSSELIKGTLKTIVLKLLTSQEKMYGYEITQKVKTLTGDKIQI
                     TEGALYPTLHALEEEGVVTTETVYIGKRIRKYYSITPKGKSKVEEKVNEFSEFMETMK
                     FLLDIKPQTGNV"
     gene            complement(2775..3293)
                     /locus_tag="PK610_08790"
     CDS             complement(2775..3293)
                     /locus_tag="PK610_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ36072.1"
                     /translation="MNTLFYIDELYMIANKQLENDENAEARKTLHEILLEDPTYGKAH
                     NAMGWLLYMKLDEYAAAEVHYKAAIKFEPTLPYVYFNYVNFLIHQNRYREAREIAFAG
                     LEVSGSSKSALWAEIGRSFELEEQYKEAMKAYYQALRKSTEKSEIERMRANIARARAK
                     IGLSLKRWLLSF"
     gene            3639..4277
                     /locus_tag="PK610_08795"
     CDS             3639..4277
                     /locus_tag="PK610_08795"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF019610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HRJ36073.1"
                     /translation="MRNFFISFIASLLVSCGGGELPVPHSINEKDSLVIIVQPLGTVS
                     WDYQHEVAEALRIHYSARVSIAETIALPEHARSPFARERYRADTLLRFLNLHLPEGTD
                     RIMGLTTVDITTTKSKEPQWKYRDWGIFGLGQCPGDACIVSSFRLGARNASAELKRER
                     LRKIAVHEIGHTFGLPHCRNEKCIMNDAAEKIATIDAEKENLCSDCKRLVLR"
     gene            complement(4289..>4849)
                     /locus_tag="PK610_08800"
     CDS             complement(4289..>4849)
                     /locus_tag="PK610_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRJ36074.1"
                     /translation="EFILTQSLVSRYKEAEKIAAIMERFECDEILFADTAEQKEALWK
                     MRRRVGEAVKSNSVYKEEDTVVPRYELPRLLEGVKCIGEKYGFKSVCYGHAGDGNLHV
                     NIIKGDLSDSDWNEKLPMGIREIFELTVALKGTLSGEHGIGWVQKNYMDIAFPQVQIE
                     LMRNIKKVFDPNGILNPGKIFPDNNN"
BASE COUNT         1359 a          962 c          984 g         1544 t
ORIGIN      
        1 aggaggataa actaattaat gctgaagcat aatgtttgta gggtgcatca aagtaataga
       61 tgttctcatt agtaactaat acaaacaaat aaccaattag aaataaatta attcctatta
      121 tgagaaatgt atgtttgtaa gtaatatttg tgtttttcaa tttaattgca gcaaaagtga
      181 ataggacgat aaaaaagaac aataatccac ctataaccac aagcaaaatt ctcaacaccg
      241 gcgtattaaa aggggttatg taacttttaa gtctttcaaa ttctggattc gccgggaata
      301 aagttgaaac gtcaatgata tggtcacctt tagctaactc ctctgataag ttaaattgaa
      361 ccgtcgaatc gtaattgtgg taaaaaaata gatttacaga tccttcttta gtatcccaaa
      421 ttgatgtgag aagtgtcccg tcgccatttc tttttctgca aacatgcata gtatctgaca
      481 gggctgtaaa ataagctaga gaaggtgtga catcctgagt ttttaaatag tcttccccgt
      541 ttcgatagcg ttcaagctgt ctcgctttta cagggtctgt aattgatgga caaaaattag
      601 ataaaacata attcgagtca ttaccttcaa ttaacatata aggctcaaca atcaggtagc
      661 tgccgtttgt gtcaatatag ataaacacat gatcatgaaa tacactatga tcgtattttt
      721 caatgtaatt cctaacttct tttgcggtcg cacatttatg aaggatatcc tccagatagt
      781 caacttcatt accaacttta agcctctttg gaaaaggatt ttgttgtttt ggatgatagg
      841 ctgccaacct ggagaatgca agtccttttt cattcattcc tgattgaggt gcggttttgt
      901 tagctccagt gggtcttgct ccggtaaatg cagctccgta ctcgtttgaa ttcttagccg
      961 ttgcaaacca tatttttgga gtagtatacc aagtgtcatg attacaacca actacagttt
     1021 ttccatcaac ggtaatttta tacatgctgc atgcatttgc ctgcttatcg aaaagaataa
     1081 taaagcaaaa aacacataag atcagaagag tccttacaat tttcatgtac tttaatgggg
     1141 ttagcgtttc tctatcaatg ccagcaacat tacttcagtt catctgcaat cactacaaat
     1201 tggaaatagt ccagtaaatc tctttgtaaa tcgcagaggt tctgatacgc attgagcata
     1261 gtactgcccg gggaaatact caggtctatt tcaagttccg gaattccttt tccggccaat
     1321 agtccgctga atcgtttttg gtaattgcgc acatcttttt caatgttcgg atacacttcg
     1381 cggtaatagg gaagcgattt ttttgtttcg tcgttcaatt catttgatcc tgaattcgca
     1441 agcaattgct gtttttcagt ttcaattcgg tccaccattt ttaaggtttc gttgaggaac
     1501 tgctcacgat cttccgattc aattttcaat ccttccagtt gtgtccggct aaaatcggtg
     1561 cgttggtgta tgccgtaatc cgaacgttca atcgttctga tttctttttg aatgaccgcc
     1621 ttacttttca ttactgtaag catcgacatt gtaagtccgg cacctgcaag cataaggatg
     1681 gatgtggtga gggtattcac tttcgaatca gcatttcgta atcctgtgat caaataaatc
     1741 ggaatgaaaa caaaaatggt gaagaacatt gatccctgtg tcattacgtt tgctccgggc
     1801 cagtgcatga ctttaaacaa caccccggta acaatgagca gacacattgg gatagcgagc
     1861 atgagaatac ttctttcgcg tcggtgtttg ctttctctga atttaagggt aaacatgata
     1921 ggtagaaaca ccacagtaaa aatggcaatt cccaatacca gtaaaatacc tgcaccgggc
     1981 agatgaagga atttcagaag ggctccaaca agaataccca aagtagaagt gataccggaa
     2041 aaaaacatga actttttcat ggcataatag tgtttgaagg ttaataacag ttgagtttct
     2101 tcttcgattt cacgcaattc ggttttgtaa aagcgtttca agacttccga caaatacgta
     2161 tcgaagtttt cgttttcatt gtattcttct tcgagaatgc aacagatatg atccaggagg
     2221 ttcaactgca ggtcctccgt tacaacgcca tggatccgaa tgacgttgtg gatcttttct
     2281 atttgcaatt cattcaggct atacattacc cgtttggggt ttaatgtcga gcaggaattt
     2341 catggtttcc atgaattcgg aaaactcatt tactttctct tccactttgc tttttccttt
     2401 tggagtgatg gaataatact tgcgaattcg ttttccgatg tatacggttt ctgttgtaac
     2461 cacaccttct tcctcgagtg catgaagggt agggtatagc gctccctcgg tgatctggat
     2521 cttgtcgccg gtgagtgttt ttaccttctg ggttatttca taaccgtaca ttttttcctg
     2581 gcttgtgagc aactttaaaa cgatcgtttt taaagtccct ttgatgagtt ctgatgaata
     2641 cataggtcaa agatacataa gatttctatg tatgcaataa aaatgcataa gaaaattatg
     2701 tatcgaattt aatatactga ttattagaat aataaaattc aaaagcccga ctcgcgaatc
     2761 gggctttgac gggtttaaaa ggacagcaac caccttttca gggacagtcc tatctttgct
     2821 ctggctctgg cgatgttggc gcgcatgcgc tcgatctcgc ttttttcggt gctttttcgg
     2881 agtgcctggt aatacgcttt catggcttcc ttatactgtt cttccaactc aaaacttctg
     2941 ccaatctcag cccataatgc agatttggaa cttccgctca cttcaagacc agcaaatgct
     3001 atttcccttg cttcccggta cctgttctgg tggatcagaa aattgacata attgaagtag
     3061 acataaggca aggtaggttc aaattttatc gctgccttat agtggacttc tgctgctgcg
     3121 tattcatcaa gtttcatata cagcagccat cccatagcat tgtgcgcttt tccataagta
     3181 ggatcttcca gtaaaatttc atgcagggtt tttcttgctt cggcattttc gtcgttttcc
     3241 aactgcttgt ttgcaatcat atacaattcg tcgatataga atagagtgtt cattgttgtt
     3301 cttaaaaaaa atgagaatag gatttttcct tccgcttgcg gaatggtact aatgggagga
     3361 atttttcaaa aaaaaatccc gactgtgggt cgggatcatg atgcaactag gaatgtcgcg
     3421 gcatgagccc gatgcacagg atttccccga ggctgtaacg attactcgtt ggtgtgctga
     3481 ggtaaaatga aatctgatac atggttgttc tgttttggtc aatgacgaca atttataaaa
     3541 gggttgccag cagaatttga aaaatgcgga taatagctaa ttgccaacaa atagaaaagt
     3601 atagtatcat ttctaaaagc gattcacgat ttttgatcat gaggaatttt ttcatctctt
     3661 ttattgccag tctgcttgtt tcctgcggag gtggagaatt acctgttccc cattccatca
     3721 atgaaaaaga ttcgctggtt atcattgtcc agcctttggg aacggtgagt tgggactatc
     3781 agcatgaggt tgcagaagca ttgcgtatcc attattctgc ccgtgtttca attgccgaaa
     3841 caatagcact gcctgaacat gcacgatctc catttgcgcg cgaaaggtac cgtgcagata
     3901 ccttactgcg ttttttgaat cttcatttgc ctgaaggaac ggataggatt atgggtctga
     3961 ccacagtaga tattaccaca acgaaaagta aagaaccgca atggaaatac cgcgattggg
     4021 ggattttcgg attaggacaa tgtccgggcg atgcctgtat cgtttcttcc tttcggttgg
     4081 gagcaaggaa cgcaagtgcg gagcttaaac gtgaacgact ccggaaaatt gcggtgcatg
     4141 agattggaca tactttcggt ttgcctcatt gtagaaatga gaaatgtatt atgaacgatg
     4201 ctgcagaaaa aatagcgaca atagatgctg aaaaagaaaa cctgtgttcg gattgtaagc
     4261 gtcttgtgct taggtaagtt ggccgtgctc agttattgtt atcgggaaaa atttttccag
     4321 ggttgaggat tccattcgga tcaaacacct tttttatgtt ccgcatcaat tcgatctgta
     4381 cctgcggaaa tgcgatatcc atataatttt tctgcaccca tccaatgcca tgctcacccg
     4441 ataaggtgcc ttttagtgcg acagttaatt caaagatttc gcgaattccc attggtaatt
     4501 tctcgttcca gtccgaatcg ctcagatcgc ctttaatgat gttgacatgc agatttccat
     4561 caccggcatg accgtagcac acgcttttga atccgtattt ttctccgatg cattttacac
     4621 cttccaataa acgaggcaat tcgtaacggg gaacaacggt gtcttcttct ttatatacag
     4681 agttcgattt taccgcttca ccaacacgac gtcgcatttt ccataaagct tccttttgtt
     4741 ctgcggtatc agcaaaaagg atttcatcac attcgaagcg ttccataatg gcggcaattt
     4801 tttctgcttc cttgtaacgg ctcaccaatg actgggtcaa tataaattc
//
LOCUS       DAOMYG010000250         4818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6132_length_4818_cov_8.230737, whole genome shotgun sequence.
ACCESSION   DAOMYG010000250 DAOMYG010000000
VERSION     DAOMYG010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6132_length_4818_cov_8.230737"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /locus_tag="PK610_08805"
     CDS             complement(<1..1392)
                     /locus_tag="PK610_08805"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ36075.1"
                     /translation="MLTHLSISNFALIDSLEVDFEKGLSILTGETGSGKSIIIEALEM
                     LLGERADLKALKNPEAKCIIEGEFLISSYGLNDFFRQHEIDYSDTTIFRREINPQGKS
                     RSFVNDTPVNLNVLKELGAFLVNVHSQHETLDIGKSVNQFEFLDAYCGNQSELEDYRK
                     EFKNWHALGLEIEQLTEQEKRSKLDLDYFTFQLEELNTAQLKQGELESSEEELATAEH
                     AEEIRLSVDKATQLMADEQQGIMVRIRELKQILGKTAAFNRSLETTGNRLESVYAELQ
                     DIEGELERIAEDVIIDPQRSKLLRERVDHLNNLLFKHRVRTIEELMALRDEIEAKVAA
                     IGSLDEQIAQKQKERSLLEEKLNKNAARLGVRRRKEIPSLEKEMNSLFQSLAMPHAEL
                     KIEISESSLNTNGNDALRFLIRTNKGASFSELAKVASGGEFSRIMLALKSIFARVKTL
                     PTIIFDEIDTGVSG"
     gene            complement(1799..2494)
                     /gene="rsmI"
                     /locus_tag="PK610_08810"
     CDS             complement(1799..2494)
                     /gene="rsmI"
                     /locus_tag="PK610_08810"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRJ36076.1"
                     /translation="MSKLWIVPTPVGNLEDMSFRAIRIMKEADLILAEDTRVTAKLLS
                     HYGIETRMQSNHTHNEHRNLDQLIERLQDGTSIALVSDAGTPGISDPGFLLVRECVKH
                     GIQVECLPGATAFVPALVVSGFPCDRFVFEGFLPQKKGRQKRLAALSTETRSIVFYES
                     PYRVVKALNEFREVFGGNRLVSVSREISKKFESTIRGTIIEAIAHFEKHEPKGEFVIV
                     LGPEKEEETVNSE"
     gene            complement(2496..3227)
                     /locus_tag="PK610_08815"
     CDS             complement(2496..3227)
                     /locus_tag="PK610_08815"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRJ36077.1"
                     /translation="MKSIDIITSHNVTIEYQLASVLDRFLAFLIDASILFGWAMIVIL
                     LASSSVYSDTYLIFIYTLFVPVYLLYHLVSEMFFGGQSVGKKALGIKVVRLDGQLPGA
                     GECILRWLFRFFDITATVGSFAALYASASDKGQRLGDMVARTVVIKLNPTTRYTISDI
                     LNIRDAQSHGEAKYPQVVRLTDEDMLLIKNTLERYRSNPNSVHRTLIADITKKVKELL
                     GITEEPQDQIAFLRSLLQEYIVLTR"
     gene            3324..>4818
                     /locus_tag="PK610_08820"
     CDS             3324..>4818
                     /locus_tag="PK610_08820"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HRJ36078.1"
                     /translation="MKETSFIQQNKDKWKRFEELNRSGQADPEELSRLFVEITEDLSY
                     ARTFYPKRSVRVYLNYLAQGVFSRIYKIKAKPFRKFAHFWTTGLPIELYRARKNLLYA
                     FLFFAVAMIIGIVSTADDYNFARVILSDNYIEMTDQFISEGDPMAVYKKSDGLPMFLR
                     IFFNNIQVAFYCFIMGIFFSIGTLFFLVYNGIMVGTFQSYFYFKGMALGTTTGTQLLM
                     TSFLTIWIHGAFEISAIVIAGAAGITIGNGLLFPGTYTRSQSLQITGRRGMKIMIGLI
                     PFFLIAAIFESWVTRYTEMPEAGKWLIILGSFFIVILYFIVLPIFVARRYPNLVALQE
                     EPVQQSTQRIELNKIRQFNELFSDSFMLMRKVWKFIMRPLFLVILPLNILLPVILTIV
                     DYSGMDYSMDTTDALIQNFPDTENHFWSSWITTFCWTLSLCSGIFAMEQYKADSKEFV
                     FNAWWKSVMKSILVVFPITLLFVFWLQNSNSTVLFLGFFLLPVLLYLP"
BASE COUNT         1335 a         1081 c          980 g         1422 t
ORIGIN      
        1 cccggaaacg ccggtatcaa tttcatcaaa aataatggtt ggcaaagttt tcacccgcgc
       61 aaaaatactt tttagtgcga gcatgatcct tgagaattca cctcccgaag caactttcgc
      121 caattcagaa aaggatgctc ccttattggt gcgaatcaaa aaacgcaagg catcatttcc
      181 gtttgtattg agtgaagatt cactgatctc aattttcaac tccgcatgcg gcatggccaa
      241 agattgaaac aaactattca tctccttttc cagcgaagga atttccttac gccggcggac
      301 acctagtcgg gcagcattct tattcagttt ttcttcgagt aaagaacgtt ctttttgctt
      361 ttgagcaatc tgctcatcca gcgaaccaat agccgcaact ttcgcttcaa tctcgtcgcg
      421 caatgccata agttcttcaa tcgtacgaac acggtgtttg aacaatagat tattcaggtg
      481 atcaacacgc tcccgaagta atttgctccg ttgcggatcg atgatcacat cctcagcaat
      541 acgctccagt tccccttcaa tatcctgcaa ttcggcatat acagattcga gtcggttccc
      601 cgtcgtttca agcgaacggt taaatgcagc ggtcttgcca agaatctgct tcaactcacg
      661 aatacgaacc atgataccct gttgctcatc agccatcagt tgagtggctt tatctaccga
      721 taatctgatc tcctctgcat gttctgcagt ggcaagttcc tcctcagagg attccagttc
      781 gccctgtttc aattgagcag tgttcaattc ctcaagctga aaagtgaaat aatcaagatc
      841 cagcttcgaa cgtttttcct gctcggttaa ttgttcaatt tccagtccaa gcgcatgcca
      901 gtttttgaat tcctttctgt aatcttccaa ctcagactga tttccacaat aggcatccag
      961 aaattcaaat tgattcacac tttttccgat atcaagcgtt tcgtgctgac tatgaacatt
     1021 caccaaaaaa gctccaagtt ctttcaggac attcagattt accggagtat cgttcacaaa
     1081 ggaacgtgac ttcccctggg gattgatctc tcttcggaat atggtggtgt cggaataatc
     1141 gatctcgtgc tgacgaaaaa aatcattcaa tccataagaa gagatcagaa actccccctc
     1201 tatgatgcat ttcgcctccg gattttttaa ggctttcaga tcggcacgtt cacccaacaa
     1261 catttccagt gcttcaatga taatcgactt ccccgagcca gtttcacccg tgaggatgga
     1321 aagacccttt tcgaaatcga cttccaatga atcaatcagc gcaaaatttg aaatggacaa
     1381 atgcgttagc ataacaacaa aattaaagca ttcctgcaac cgaaagaccg aactgtaaac
     1441 aagtcggtac tttttgttca aaattgatca tggtccaaag aaaaagagcc tgcacgcaat
     1501 gtatctgcgt tgagtgggag caatttgttt atttccaatt tccaattaat taacttccaa
     1561 tttccgaatt tccaatttcc aattggattg tggagaattg gtagttggag tgacagattg
     1621 gagattgtgc ttcagggtgg ttggggtgat ctattttcaa tttccaattt cctaatttcc
     1681 aatttccaat tatttaattt ccaattatct aatgccaatt attgggctaa accatatata
     1741 ctctcacaca aagactcagt gtccagaaat ctgttcacct ttcactgttc accattcact
     1801 attcactgtt caccgtttcc tcttcttttt caggtccaag tacaatgacg aattctcctt
     1861 taggttcatg tttttcgaag tgagcaatag cttcgatgat ggtgccgcga atggtggatt
     1921 caaacttttt actgatttcg cgggaaacgg ataccagtct gttcccgcca aatacttcac
     1981 gaaattcgtt gagtgccttt accacacgat agggggattc gtaaaaaacg atacttcgtg
     2041 tttcggtcga taaagcggcg agtctttttt gccttccttt tttctggggc agaaatcctt
     2101 caaatacaaa acgatcgcaa gggaatccgc ttaccaccaa tgcaggaaca aatgctgttg
     2161 caccgggaag gcattcaacc tgaataccgt gttttacaca ttcccgaacc agcaaaaatc
     2221 cgggatcgga aatcccgggt gtaccggcat cacttacaag cgcaatcgat gttccgtcct
     2281 gcaatcgttc aatcagctga tcgagatttc ggtgttcgtt atgggtatga ttgctttgca
     2341 tacgggtttc gattccgtaa tgcgataaca attttgcagt aacacgggta tcttctgcga
     2401 gaatgagatc cgcttctttc ataatgcgaa tcgcacgaaa gctcatgtct tcaagattgc
     2461 caacaggcgt gggaacgatc cagagtttac tcattttatc gggtaagaac gatgtattcc
     2521 tgaagaagac ttcgcaaaaa tgcaatttga tcctgtggtt cttcagttat tccaagaagc
     2581 tccttcactt tttttgtgat atcggcgatg agggttcggt gcacgctgtt gggattggaa
     2641 cggtaacgtt caagtgtgtt ttttatcagc agcatatcct catcggttaa acgaaccacc
     2701 tgcggatatt tagcttcacc atgactttga gcatcgcgaa tattgagaat gtcggaaatg
     2761 gtataccgcg ttgtgggatt cagtttgatc accactgtgc gtgcaaccat atcacccaga
     2821 cgctgacctt tatcactcgc tgaagcgtac aaagcagcga aagaacctac agttgcagta
     2881 atatcgaaaa aacgaaacaa ccaacgaagg atgcattcac cagcacccgg caactgacca
     2941 tccagccgaa caacttttat tcccagcgct ttctttccga cggattgtcc gccgaaaaac
     3001 atttcgctca ccagatgata caagaggtaa accggaacaa aaagcgtgta aataaaaatg
     3061 agataggtat ctgaataaac tgaggaggaa gccagaagaa ttacaatcat tgcccaaccg
     3121 aacagaatgg atgcatcgat aaggaaagcc agaaagcgat cgagaaccga ggcaagctgg
     3181 tattcgatgg tgacattatg actggttatg atatcgatgg atttcattgc tcaaagcaag
     3241 tttagcagag aaagcactaa gttacactaa gattttattg aacagtaaat cgggattggt
     3301 tttttgctat ttttaggcaa gccatgaagg aaacctcctt tatacaacag aacaaggaca
     3361 agtggaaacg ctttgaagaa ctcaaccgga gcggtcaggc tgatcccgag gaattgagca
     3421 ggttgtttgt ggaaattact gaggatcttt cctacgcccg aaccttctac cccaaacgat
     3481 cggtgcgcgt gtatctcaac tatcttgcgc agggagtatt ttcaagaatc tacaagataa
     3541 aagcaaaacc attccgcaag tttgcacatt tctggacaac aggtttacca atagaactgt
     3601 accgtgcccg caaaaatcta ttgtatgcat ttttgttttt tgcagtggcg atgatcattg
     3661 gaattgtttc cacggccgac gactacaatt ttgcgcgggt gattttatcg gacaactata
     3721 ttgaaatgac cgatcaattc atttcagaag gagatccgat ggcagtgtat aaaaagagcg
     3781 atggcttacc catgtttctt cgcatcttct ttaacaacat acaagtggca ttctattgtt
     3841 tcatcatggg gatcttcttc agcattggga cattgttttt tctggtttat aacggaataa
     3901 tggtgggaac ctttcagtcc tatttctatt ttaaaggaat ggcattgggg acgacaacag
     3961 gcacacagtt gctgatgaca agtttcctta ccatttggat acacggtgca ttcgaaattt
     4021 ctgcgatcgt tattgcagga gcagccggca ttactattgg caacggactc ctctttcccg
     4081 gaacgtatac ccgcagtcag tcattgcaga tcaccggaag acgcggaatg aaaattatga
     4141 tcggactgat tccgtttttt ttgattgccg caatttttga aagttgggtc acccgctata
     4201 ctgagatgcc cgaagcagga aaatggctga tcattctggg atcctttttc attgtgattc
     4261 tctattttat tgttctccca atttttgttg ccagacgata tccgaacctg gtagcgcttc
     4321 aggaagagcc tgttcaacaa tcaacacaac gcatcgaact gaataaaatc cgccagttta
     4381 atgaactctt cagtgattcg tttatgctga tgcgaaaagt atggaagttc attatgcgtc
     4441 ccctgttcct tgtgattcta ccgttgaaca tccttcttcc tgtaattctt acaatagtag
     4501 attacagcgg aatggattat tcgatggaca ccactgatgc attgatacag aactttccgg
     4561 ataccgaaaa tcatttctgg tcctcatgga tcactacgtt ttgctggaca ctttcacttt
     4621 gttccggaat atttgccatg gagcagtaca aggcagattc aaaagaattt gtattcaatg
     4681 cctggtggaa atcggtaatg aaatccattc ttgttgtttt tccgatcact ttactttttg
     4741 tgttttggct tcagaacagc aattcaaccg tattgtttct tggtttcttc ctattaccgg
     4801 ttttgttgta cctcccct
//
LOCUS       DAOMYG010000251         4810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6144_length_4810_cov_7.630284, whole genome shotgun sequence.
ACCESSION   DAOMYG010000251 DAOMYG010000000
VERSION     DAOMYG010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6144_length_4810_cov_7.630284"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="PK610_08825"
     CDS             <1..1200
                     /locus_tag="PK610_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36079.1"
                     /translation="DSALTYTNTGVGVQLAKVSIGDAQVAFDDDWFFSYSVEERVNIL
                     SLTGDNPADTSAAVSRLFRNDPFFKLESRKVSQVDFSSFPSRHLIILNEVPVLSSGLT
                     DEVVRFVNAGGNVLLFPSIRSELSSLNNFLVQCGAAGFTGKDTSAIRPAPIDVRTPFF
                     SRMFEREPREIDLPQFRFRYISETRSMPGKEQLWKMRNGDEFLSRYSKGKGFIYVSTV
                     PLSEKAGNFSKHALFVPVVLRIAEWSQGSGINMQLIGRDLPIEIRSTLPGGDATFQLE
                     NEAGDFSVIPEMRNTGGGVSLYVHGQIREAGNYKVKKGNDLIAGIGFNYDRKESDLAC
                     YETEEISKILESGGMSDFTLFDKVEEGKGITLEALDDTREYWRLCILLALLFLLAEVL
                     LIRLMKQ"
     gene            1211..2473
                     /locus_tag="PK610_08830"
     CDS             1211..2473
                     /locus_tag="PK610_08830"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRJ36080.1"
                     /translation="MKTLFRNATVSDKNSSFNGKKVDILIASGKIEKIASSLTEKADE
                     EIDLKGAFVSPGFLDLQASYCDPGLEHKETLQTGAAAAVKGGFTHVLVMPDSQPVMSG
                     KTIVDYISNHSKSLPVNMIPCGSVSQKFDGENLAEMYDMWQSGARAFFDVNHYVRAGL
                     MSRALLYAKNFGGKVFSLPFDPSLAPGGLMHEGVVSTSLGLKGIPELAEEIVVQRDLH
                     LAAYHNAPVHFAGISAPRSFEYISEAIKKGTEVTSHLPAFLLFFTDEDLVGFDTHLKV
                     IPPLRSARVKDALIDAVVKGKCDAIASHHTPQDTESKSVEFDHAEFGMIAQETAFSAA
                     FTVLEPKMKVEEITALFSHGPRRVLGLPEIKIEAGYNADLSIFSTIEEWVYAKSDTRS
                     LSANTPFEGKKLKGKIIGTFTKGSYNKA"
     gene            complement(2479..3555)
                     /locus_tag="PK610_08835"
     CDS             complement(2479..3555)
                     /locus_tag="PK610_08835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ36081.1"
                     /translation="MFRRYFFFSALLVATNANAQISITSADMPVANEVYPISTTIDQW
                     SIDVTQTGSNHIWDFSFLTRVSQLLDTTFEVQDTPTAYQFFFNNNFVYPAHVAQFARR
                     GQDLDLFGFLTITNVFDFFSTSNNKYKNVGYGARINGFPNSVRNQPVDTIYKFPLTDG
                     TPVYGSYSVSLVNIPTILYYRQQKTMSNATVDGWGSITTPLGTFDCIRVKMTIDIRDS
                     LSMDVGGFPLNFGVNRPTETQYHWLANGKHVPVLQINTSFGVITTIRYLDTIANLSVE
                     ENRVLPIVAYPNPVSDILLVEHSREGGWIELYDMNGKKIHTQNFTSGTDVSYVNVHTY
                     PAGNYIVRMQSGKEFRTGKITVTH"
     gene            complement(3625..>4810)
                     /locus_tag="PK610_08840"
     CDS             complement(3625..>4810)
                     /locus_tag="PK610_08840"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRJ36082.1"
                     /translation="IISKVKEFRKQKDPRKKDFSPTLLDFGSVRFYIARHFGFCYGVE
                     NAIEISYRAIEENPGKRIFLLSQMIHNPEVNADLERNGIRFIMDTHGKHFLSWDEINK
                     DDIVIIPAFGTTLEIEQLLQEKGIETQKYNTTCPFVEKVWNRAEKLGSESYTIIIHGK
                     QTHEETRATFSHSKKNAPSIVIKDMKEAQILGDIVLGKMDLSSFSTYFGDRCSQGFDP
                     ATDLQRVGVINQTTMLATETQEIADYFKKVMAEKYPVDNSRDHFADTRDTLCYATNDN
                     QQATLAMMDQVADLALVIGGYNSSNTTHLVELLEQKFSTYFITGEKEIRENGSISYFD
                     IHHHEIKQADHFLPQKQPLNIIVTSGASCPDAVVDRVLQKVLMLLNAERSIEEVLTDL
                     SK"
BASE COUNT         1349 a          959 c         1113 g         1389 t
ORIGIN      
        1 gattctgcac ttacctatac caataccgga gttggggtgc aactggcaaa agtaagtatt
       61 ggagatgccc aggtggcgtt tgatgatgac tggttttttt cctattcggt ggaagaacgc
      121 gtaaatattc tttcgcttac aggagacaat cctgccgata caagtgcagc ggtttcccga
      181 ctgtttcgta acgatccctt ttttaaactg gaatcgcgga aagtatcgca ggtagatttt
      241 tcgagttttc cttcgcgcca tttgatcatt ttgaatgaag ttcccgtttt aagcagcgga
      301 ctaaccgatg aggtcgttcg ttttgtgaat gctggaggta atgtgctttt gtttccttct
      361 atccgttcgg aacttagttc acttaataat tttttggtac aatgcggtgc tgcaggattt
      421 accggaaagg ataccagtgc gatacgacct gcaccaattg atgtgcgcac tccgtttttt
      481 tcccgcatgt ttgaacgcga gccgcgcgaa atagatcttc ctcaattccg ctttcgttac
      541 attagcgaaa cacgatcgat gccgggcaag gaacaactat ggaaaatgag aaacggtgat
      601 gaatttttat cccgttattc caaagggaaa ggctttatct atgtgagcac ggttccgctt
      661 tcagaaaagg ccggtaattt ttcgaagcac gctttgtttg tcccagtggt tttgcgcatt
      721 gcggaatgga gtcagggttc gggtatcaat atgcaattaa tcggaagaga tttgcccatt
      781 gagataagaa gtactttgcc cggaggggat gcaacgtttc aactggaaaa tgaagcaggc
      841 gatttttcag tcattcccga aatgcgtaat accggtggtg gagtgtcgct gtatgtgcac
      901 ggacagatcc gtgaagcggg gaattacaaa gtgaaaaagg gaaatgatct gattgcagga
      961 attggattta actacgacag aaaagagtcg gacctggctt gttacgaaac tgaggagatc
     1021 agtaaaattc tggaatccgg cggtatgagt gatttcactt tgttcgataa ggtggaagaa
     1081 ggaaaaggaa ttacattgga agcactggat gatacccgcg aatactggag gttatgtatt
     1141 ctattggcat tactctttct attggcagaa gtactactta tacgattaat gaaacaataa
     1201 gcgataaacc atgaagacac tttttcgcaa tgcaacggtt agcgacaaga acagttcgtt
     1261 caacgggaaa aaagttgata ttctgattgc ctccggaaaa attgaaaaaa ttgcatcctc
     1321 gctgaccgaa aaggcggatg aggaaattga tctgaaaggt gcttttgttt ctcccggatt
     1381 tctcgattta caggcttcgt attgcgatcc cggattagaa cacaaggaaa cgcttcaaac
     1441 cggtgctgcg gctgcggtaa aaggcggatt tacccatgtt ctggttatgc cggatagtca
     1501 gccggtgatg tcgggtaaaa ccattgtgga ttacatcagc aatcactcta agtctttacc
     1561 cgtaaacatg attccatgcg gatcggtttc gcaaaaattc gatggcgaaa atctcgctga
     1621 gatgtacgac atgtggcaat cgggcgcaag agcgtttttt gatgtgaatc attatgtgcg
     1681 cgcaggattg atgtcgcggg cattgctgta cgcgaaaaat ttcggcggaa aagtattcag
     1741 tctgcccttt gatccttctc ttgcaccggg tggattgatg catgaaggtg tggtaagcac
     1801 ttcactcgga ctaaaaggaa ttcctgaact cgcagaagaa attgttgtgc agcgtgatct
     1861 gcatcttgca gcctaccaca atgcgcctgt acactttgct ggaatctcgg caccacgatc
     1921 attcgaatac atcagtgagg ccatcaaaaa gggcacagaa gtaacatctc acctgccagc
     1981 ctttttgctt ttctttaccg atgaggatct tgtaggtttt gatacccatt taaaagtcat
     2041 tccgccattg cgatctgcca gggtaaaaga tgccctgatt gatgctgttg taaaagggaa
     2101 atgtgatgca attgcttcgc accatactcc acaggataca gaaagtaagt cggtggaatt
     2161 cgatcatgcc gagtttggaa tgattgcaca ggaaactgct ttttctgctg catttacagt
     2221 tttggaacct aagatgaaag tagaagaaat aactgcgctc ttcagtcacg gaccacgtcg
     2281 tgttttaggt ttgcctgaaa taaagatcga agcaggatac aatgccgacc tgagtatctt
     2341 ctcaacaatt gaagaatggg tttatgctaa atcggatacc cgatcattat cggctaatac
     2401 gccgtttgaa ggtaaaaaac tcaagggtaa aattatcggt acgtttacca aaggatcgta
     2461 caataaagcc tgatattttt agtgcgtcac ggtaattttt cctgtgcgaa attctttacc
     2521 actctgcatg cgcacaatat aatttcctgc tggataggta tgcacattaa cataagaaac
     2581 atccgttcct gaagtgaaat tctgagtatg aattttcttt ccgttcatgt cataaagctc
     2641 aatccaccct ccttcgcgcg aatgttctac caataaaata tcgctcacag gattgggata
     2701 ggcaacaatt gggagtaccc tgttttcttc aaccgataaa ttggcaatcg tatcaaggta
     2761 acggatcgta gtgataacac cgaatgacgt gttgatctgc aataccggaa catgttttcc
     2821 gtttgccagc caatggtatt gggtttcggt tggacgatta acgccaaaat ttagagggaa
     2881 tcctccaaca tccatactca gagaatcgcg gatatcgatg gtcattttta cacggataca
     2941 atcgaatgtt cccaaaggtg tggtaataga accccaaccg tcaacagttg cattcgacat
     3001 cgttttttgc tgacgataat agaggatagt tgggatattt acaagactta cggaatagga
     3061 accgtatacc ggagttccat ctgttaaggg gaatttgtaa atggtatcga caggctgatt
     3121 gcggaccgaa tttggaaaac cattgatgcg cgctccgtaa cctacgtttt tgtatttgtt
     3181 gttggaggta ctgaaaaaat caaaaacatt tgtaatggtt agaaaaccaa aaagatcaag
     3241 gtcttgccct ctacgtgcga attgtgctac gtgtgcgggg taaacgaagt tattgttgaa
     3301 gaaaaattga taggccgttg gagtgtcctg aacttcaaaa gttgtatcca aaagttgaga
     3361 tacacgggta aggaaggaaa aatcccaaat atgattggaa cctgtttgtg ttacatcaat
     3421 actccattgg tcgatggtag ttgaaatagg gtacacctca ttcgcaaccg gcatatctgc
     3481 agaggtaata gaaatctgtg catttgcatt tgttgcaact aaaagcgcag aaaagaagaa
     3541 atagcgacgg aacataatca gggtttttag cccggccaag atacaaaatt ggcaatcaaa
     3601 aaccagacgg cttcctggga ggaatcattt acttagatcg gtcagtactt cctcgatgct
     3661 ccgttcagca ttaagtagca tcagtacttt ttgtaagacc ctgtcaacca ccgcatccgg
     3721 acaagaagca ccgctggtta caattatgtt caagggttgc ttttgtggca ggaaatgatc
     3781 cgcctgtttg atttcgtggt ggtgaatatc gaaataggag atgctgccat tttctctgat
     3841 ttctttttcg ccggtgataa agtaggtgct gaatttttgc tccagtaatt caaccaaatg
     3901 tgtggtattg gaactgttgt aaccgccaat aaccaaagca agatctgcca cttgatccat
     3961 cattgctaga gtagcttgtt gattgtcatt ggtagcgtaa caaagtgtat cacgggtatc
     4021 cgcaaaatga tcacgggaat tgtcgaccgg atatttttcc gccatcactt ttttaaagta
     4081 atccgcaatt tcctgagtct ctgttgcaag catggtagtt tggttgatca ctccaactct
     4141 ttgcagatca gtagcagggt caaatccttg cgaacaacga tcaccaaaat aagtgctgaa
     4201 tgaacttaaa tccattttgc caagcacaat atccccaaga atctgtgctt ctttcatatc
     4261 ctttattacg atagaaggag cattcttttt actgtgtgaa aaggtggctc ttgtttcttc
     4321 atgcgtttgt tttccgtgaa tgataatggt gtaggattcg cttcccagct tttctgcgcg
     4381 gttccagacc ttttctacaa aggggcaggt ggtgttgtat ttctgtgttt cgattccttt
     4441 ttcctgaagc agttgctcaa tctccagcgt agttccgaat gctggaataa ttacaatatc
     4501 atctttgttg atttcatccc aggaaagaaa atgtttgcca tgcgtatcca taatgaaacg
     4561 gatgccattt cgctccagat cggcatttac ttccggatta tgaatcatct gactcagcag
     4621 aaaaatacgt ttacccggat tctcctcaat ggcacggtaa gagatttcga tggcattttc
     4681 aacaccgtaa cagaacccga aatgccgggc aatataaaaa cgaacagaac caaagtcgag
     4741 aagggtagga ctgaaatcct ttttacgcgg atctttttgc ttacgaaact ctttgacctt
     4801 gcttattata
//
LOCUS       DAOMYG010000252         4789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6188_length_4789_cov_8.380439, whole genome shotgun sequence.
ACCESSION   DAOMYG010000252 DAOMYG010000000
VERSION     DAOMYG010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6188_length_4789_cov_8.380439"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2557
                     /locus_tag="PK610_08845"
     CDS             <1..2557
                     /locus_tag="PK610_08845"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRJ36083.1"
                     /translation="NSIAIGYLKDVGEVLWVTALDGVHAFSILKREWIYFDDYINFLG
                     GDDYRVVASGIFHGNEFAFLMAAGLGVINQEGETSFLPVKDFKDHGVYTLSKKGDQYW
                     IININGKGVYVSQGGRIVPFLSESLNSAINGRKMTFNKWIDDEFFIGTHSGLIIYNDR
                     TNTFHEYFHSTPVSGVIKDREDNFWMTSLSEGVFRIPDFRNEVWRIQASSGRTIPITK
                     INVNREFVFCSSGQGHVGVLNQATKEWKVIPMPIQADPQTLFIDVDGRAYISIMNTIY
                     ELKGEQFIPLVTTVSSCKTIFRINGGYLFGSSIGVFFLPEISDRFPLKTLSRFWTREF
                     QLHPSRPGAYLAATDNGVLEILPQSDSFEVRTFCMEGQQVLSIQVDTTHQSLYTLTAS
                     GELVRIDQEGEQLIKKFDEADIPNKIRLQDSGIVVATAHGLITIRFGDWKSQRITRRE
                     GLVADEINGLVVVENGFWLATARGLQFIPSHAKDAISDASLYLKTLKTDEQYHDPSAF
                     LHLDFGQSLTFFPEFSCYRFADAADYAYRILPHDTTWRFIPGNTESFTIPSLQSGEFT
                     IELGLVYEGEMLVEKPIYIKGYVLAPFWQRWWFYLLIGLFALVVAFTLFRIRLKFIRK
                     KQLQELNRIHLETELKLSQQTALKSQMNPHFLFNVLNSIKGFIYENDKKNASSYLSDF
                     SVLVRHVLELSARPQIRLSEELQVIEPYIRLEAMLLDGDFSYAIHVDDSVDPDNIAIP
                     PLIIQPYVENAFKHGLRHKQGEKRLEINVGVENNQLIIAIADNGVGRIRSGEINKDRS
                     QHRSFGSDASRKRIALFSDGNSEQQAVEVVDRFDQEGKATGTIYILKIPLHEF"
     gene            2547..3308
                     /locus_tag="PK610_08850"
     CDS             2547..3308
                     /locus_tag="PK610_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ36084.1"
                     /translation="MNSEKINAVLIDDSSQARKLLRLMLAELAPDVNVLGECSDLSEG
                     VKSIREYKPDVVFLDIEMPGKSGLQILEELPRDEMQYEIIFTTAYDEYALRAFRLSAL
                     DYLLKPISETELNEAVKKIRQRKQLYSSSERIQALNTNLQQKEASVICVPIQNGFARI
                     PLESILYFEADGSYVNIVTVEGKPRTISKNLKYIEKTLEGNSRFLRVHRSYLLNINQV
                     LEYNRSGRGTILMKNGHSIDLARDRKEAFLKVFGT"
     gene            complement(3464..4279)
                     /gene="menB"
                     /locus_tag="PK610_08855"
     CDS             complement(3464..4279)
                     /gene="menB"
                     /locus_tag="PK610_08855"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HRJ36085.1"
                     /translation="MRNWTKIKDYEEIKFEFCEGVAKISINRERFRNAFTPLTVMEMI
                     DAMNYCRDEAKVDVIVLTGVGDKAFCSGGDQNVRGHGGYVGGDKIPRLNVLDLQKIIR
                     SIPKVVIAMVNGYAIGGGHVLHVVCDLSIASENAIFGQTGPRVGSFDGGFGASYLARC
                     VGQKKAREIWYLCDQYNAQQALDMGLVNTVVSLDKLEDEVMIWAGKIREKSPLAIRML
                     KSSFNAELDGQTGIQELAGNATLLYYLSDEAKEGKNAYIEKRKPDFSKFPKFP"
     gene            complement(4335..>4789)
                     /locus_tag="PK610_08860"
     CDS             complement(4335..>4789)
                     /locus_tag="PK610_08860"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRJ36086.1"
                     /translation="SNRGASGIDGCTSTASGAAHASKRPTTLITGDIGFFYDSNALWN
                     NYLHSSLRIIVINNGGGGIFRIIDGPEHEEEIEKLFETRQQLSAEHLCKAFGLNYFKA
                     ESKLELDLQLKAFYDSSNQQASVLEVFTPTRENPKILKSYFDHLKANS"
BASE COUNT         1335 a          934 c         1039 g         1481 t
ORIGIN      
        1 taatagtata gcaattggtt atctgaagga tgtcggtgag gttttatggg tgactgcctt
       61 ggatggagtt catgcatttt ccatcctaaa aagggagtgg atctattttg atgactacat
      121 caattttctg ggtggtgatg attatcgtgt tgtggcatcc ggaatttttc atggtaatga
      181 atttgcgttt ttgatggctg ccggtctagg tgtaattaac caggaaggag agacatcttt
      241 tcttcctgta aaggatttta aagaccatgg agtttataca ctttcaaaaa aaggagatca
      301 atactggatt atcaatatta acggtaaagg tgtttatgtc agccagggag ggcgaattgt
      361 ccctttcctt tcagagtcgt taaattctgc gataaatgga cgaaagatga cctttaataa
      421 atggattgac gatgagtttt ttattggtac acactccgga ttgattattt ataatgacag
      481 gacaaacact tttcatgaat attttcattc cacccctgta tcgggagtga tcaaggatcg
      541 tgaagataat ttttggatga cctccttgtc tgaaggggtt tttcgaattc ctgattttcg
      601 taacgaagta tggagaattc aagcatcgtc cgggcgcacg attccgataa caaagattaa
      661 tgttaatcgg gaatttgttt tttgttcaag cggtcagggt catgttggtg ttttgaacca
      721 agccactaag gaatggaaag tcatcccaat gcctatccag gccgatccgc aaactttgtt
      781 tattgatgta gatggtcggg cctatatttc tataatgaac acgatttatg aactgaaagg
      841 agaacaattt attcctctgg ttactactgt ctcttcgtgt aaaactattt ttcgtattaa
      901 tggtggttat ttgtttgggt cttctattgg tgtgtttttt cttccggaga tcagtgatcg
      961 ttttccattg aaaaccttat ccagattttg gacccgcgaa tttcagttac acccatcaag
     1021 gccgggagca tatcttgcag caacggataa tggtgttctt gagattcttc cgcaatctga
     1081 ttcttttgaa gtgcgtacat tttgtatgga gggtcaacaa gtattatcga tacaagtgga
     1141 cactacccat cagtctcttt acacattaac ggcatccgga gaactagtga ggattgacca
     1201 agagggtgaa caactcatta agaagtttga tgaagcagat attcccaata aaattcgact
     1261 tcaggattcc ggtatagtag tagctactgc acatggattg ataaccatcc gttttggtga
     1321 ctggaagtcg caacgcatca ccagacgcga aggtcttgtt gcggatgaaa tcaatggact
     1381 tgttgttgtt gaaaatgggt tttggttggc tacagcaagg ggtttgcagt tcattccgtc
     1441 tcatgccaag gatgccatct cagatgcatc cttgtacctt aaaacattga agaccgacga
     1501 gcaatatcat gatccatctg catttcttca tttggatttt ggccaatcgt taacattctt
     1561 ccccgagttt tcctgttatc gttttgctga tgctgctgat tatgcctatc gcattttacc
     1621 tcatgatacg acctggagat ttattccggg aaataccgaa tcctttacta ttccgtcact
     1681 tcaatccggt gagtttacaa tcgagttggg tttggtttat gaaggcgaaa tgctggtgga
     1741 aaaaccaatc tatataaaag gttatgtgct tgcaccgttc tggcagcgat ggtggtttta
     1801 tttattgatt ggtttgttcg cattagtcgt tgcgtttaca cttttcagaa tccgattgaa
     1861 atttattcgc aaaaaacaat tgcaggaatt gaaccggatt catttggaaa cagaattgaa
     1921 actatcacag caaactgcgc taaaatcaca aatgaatcct cattttttgt tcaatgtgtt
     1981 gaactctatc aaaggattta tttatgaaaa tgataaaaag aacgcgtctt cttatttaag
     2041 cgatttttct gttttggttc gtcatgtcct ggagttgagt gcacggccgc aaatcagatt
     2101 gtcggaagag ctgcaagtta ttgagcctta catcagattg gaagcgatgt tactcgacgg
     2161 agatttttct tatgcaattc atgtagatga ttccgttgat ccggataata ttgcaattcc
     2221 tcctcttatt attcagccct atgttgagaa tgcctttaaa cacggcttaa gacacaaaca
     2281 aggcgaaaag cgtttagaga taaatgtcgg agtggaaaat aaccagttaa tcattgctat
     2341 tgctgataac ggagtgggaa gaattcgttc aggagaaatc aataaagaca gaagtcagca
     2401 ccgttccttt ggttccgatg catcacgcaa acgaattgca ttattttcgg acggaaatag
     2461 cgagcaacag gcagtagaag tggttgatcg ttttgatcag gaaggaaaag ctacaggtac
     2521 catatacatc cttaaaatac ctttacatga attctgagaa aataaatgcg gtgttgattg
     2581 atgattcttc acaggcacgg aaattattgc gattgatgct tgcagaatta gcgcctgatg
     2641 taaatgtgct tggcgaatgt tcggatttaa gtgaaggagt aaaatcaatt cgggaatata
     2701 aacctgacgt tgtatttctc gatattgaga tgccgggtaa atcaggactt cagattttgg
     2761 aggagcttcc ccgtgatgaa atgcaatatg aaataatatt tacaacagct tacgacgaat
     2821 atgctttgcg tgcatttcgc ttatctgcat tggattatct tttaaaacca atcagtgaaa
     2881 cagaacttaa cgaagcagta aaaaagatcc gtcagcgaaa acaattgtat tcttcatccg
     2941 aaagaattca ggcactcaat acaaatcttc agcagaaaga agcaagcgta atttgtgtgc
     3001 ctattcaaaa tggtttcgca cgtattcctc tggaatcaat cttgtatttc gaagcggatg
     3061 gttcctacgt gaatattgta acagttgaag gcaaaccgcg gacgatctca aaaaatttga
     3121 aatacatcga aaaaacactt gaagggaact cacgctttct tcgggttcac cggtcgtatt
     3181 tgctgaatat caaccaggtt ttggaataca accgatccgg acgaggtacc atcctcatga
     3241 aaaatggcca ttccatcgat ctggcgcgcg atcgaaaaga ggctttttta aaggtttttg
     3301 ggacctgaaa tagcggttcg taaactcaaa cggcactttc acaaagtcgg tattgccttt
     3361 tacacaaagt gtggctatgt tcggtttcgc aagcaggcga atgaggaaaa gccccggaaa
     3421 gatttacttc ttatggggct ttttctgttt ctaactttgc tgcttatggg aacttcggga
     3481 atttgctgaa atcaggttta cgcttttcga tatatgcgtt tttcccttct ttggcttcat
     3541 cgctgaggta atacaataaa gttgcatttc ctgccaactc ctgaataccg gtttgtccgt
     3601 cgagctctgc attgaaggat gatttcagca tgcgaatggc caatggcgat ttctcacgga
     3661 tcttcccagc ccaaatcatc acttcgtctt caagtttatc gagagaaaca actgtattca
     3721 ccaagcccat atccaaggcc tgctgtgcat tgtactggtc gcagaggtac cagatttcac
     3781 gtgctttctt ttgaccgaca caacgtgcca gatacgatgc cccaaaacca ccgtcgaaag
     3841 aaccaacgcg tggaccggtt tgcccgaaga tggcgttttc gctggcgatg gaaagatcgc
     3901 aaaccacatg caaaacatgt ccaccaccaa tcgcgtatcc gtttaccatg gcaatcacca
     3961 ctttcggaat ggaacggatg atcttttgca gatcaagaac attcaaacgg ggaattttat
     4021 cgcctccaac atatccgcca tgaccacgaa cgttttggtc accacccgaa caaaatgctt
     4081 tatcgccaac accggtgaga acaatgacat ccaccttcgc ttcgtcgcgg caataattca
     4141 tcgcatcgat catctccata acggttagcg gagtaaacgc attacggaaa cgctcacggt
     4201 tgatgctgat cttagcaacg ccttcacaga attcaaactt gatttcttcg tagtccttga
     4261 ttttagtcca gtttctcatt ggattttggt attgatggtt ttgattttat tttattgtgg
     4321 tatgggcaat tcaattacga attagctttc aaatgatcga aatagctttt taaaattttt
     4381 ggattttccc tcgtaggtgt aaacacttcc agtacggagg cttgctgatt tgacgaatcg
     4441 taaaacgctt tgagttgcaa atcgagttcg agtttactct cggctttgaa ataatttaat
     4501 ccaaacgctt tgcaaaggtg ttctgcagaa agctgttgac gtgtctcaaa taatttttca
     4561 atttcctctt cgtgctctgg tccgtcaatg atgcggaaaa ttcctcctcc gccattgtta
     4621 atgacaatta ttcgcaatga agaatgaaga taattgttcc aaagtgcatt actatcgtag
     4681 aaaaatccaa tatcaccggt aatgagagtg gtcggacgtt tacttgcatg agccgctcct
     4741 gatgctgtgc tggtgcatcc atctattccg cttgcaccac ggttgctgt
//
LOCUS       DAOMYG010000253         4781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6216_length_4781_cov_8.185569, whole genome shotgun sequence.
ACCESSION   DAOMYG010000253 DAOMYG010000000
VERSION     DAOMYG010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6216_length_4781_cov_8.185569"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1824)
                     /locus_tag="PK610_08865"
     CDS             complement(<1..1824)
                     /locus_tag="PK610_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ36087.1"
                     /translation="MSERILKALMQLFAIIARVEDEDGPEAESGSTDGGGRRIVEIFL
                     KSQLNQELVDEYLRLFDEYLEAHQGKSQKKDGAKKRTSVNSVKVLRICTQINEELAQK
                     QKIIVLVRILEFINENQSVTEQELEFASTVAETFNISEEEFRRCKNFVDNKEDQTEDG
                     AYLLYINNSKENPFKEAHHIYSEGIQGHIRVLRIPSLGIYFLRYYGNSEIYLNGINIT
                     PLRVYILTQGSSIRSSRVQPIYYSDIISKFLSDTSVSKIVFKVDEIMFHFKGGKIGLH
                     PLTFSEESGKLLGIMGGSGAGKSTLLNVLNGNYAPTRGAITINGVNIHSESKKIEGVI
                     GYVSQDDLLIEELTVFQNLFYNAKLCFGNLNDAQITKLVLNLLTALGLYETKDLRVGN
                     PLDKTISGGQRKRLNIGLELIREPAVLFVDEPTSGLSSRDSENIMDLLKELALKGKLV
                     FVVIHQPSSDIFKMFDRLLILDVGGFPIYYGNPVDAVIYFKTLVNHVNCNESECPTCG
                     NVNPEQIFNIIESKVVDEYGNATQNRKVSPKEWNELYTEKIGNQLPEAPAQKDIPEST
                     FKIPNKIKQYWVYLVRDVLSKLTNTQYILINVFEAPALALIL"
     gene            complement(1902..2357)
                     /locus_tag="PK610_08870"
     CDS             complement(1902..2357)
                     /locus_tag="PK610_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36088.1"
                     /translation="MRTLVLSVIVLFSAAFSGNAGAQSCDSLTNVCKGHLAKPAKKDK
                     GSIFISDGQTYRALLDEEQTAEFTTTFYGGSTYRIATSAGTKDNYVIFEVYDKERNLL
                     FSNADYGNAPYWDFKVESTVDVIIEARLDPNKKSSGCTVMLIGFKQLKK"
     gene            complement(2354..3274)
                     /locus_tag="PK610_08875"
     CDS             complement(2354..3274)
                     /locus_tag="PK610_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36089.1"
                     /translation="MQNRTRGITSISFIALGVSMLMFSACGDSNTNEQDVTKVDPKVL
                     DTSDAMGISINGRLFSIPSPFQTAELIKSTGAPYNAAMLNSTDQTANYSTKFNKALNL
                     GVYGADLGYATMYDNTTDAMSYLSSVEQLSKELDMGGAFSEDIIDRFSENMGNKDSML
                     VIVSDAYRNGDEYLKTNDRAQEAGLVLTGGWIEALYFACEVVNKTKDQKIINRIGEQK
                     TTLGNLIELLEQYNTEESIANLIGEMQSLYTLFDQIKVTYTYQKPETDAENQVTVIKS
                     STKVDITPDNLNNITLKVKELRTKIVQSEL"
     gene            3431..4399
                     /gene="pfkA"
                     /locus_tag="PK610_08880"
     CDS             3431..4399
                     /gene="pfkA"
                     /locus_tag="PK610_08880"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRJ36090.1"
                     /translation="MKKIAVFTSGGDSPGMNACIRAVVRTGIYHDLEVVGILQGYEGM
                     IDGDFIPMDRKSVANIINRGGTILKTARSPRFLEPEGRKAAYQQLKKAGIEGVIAIGG
                     DGTFTGARVFSEICEIPIVGCPGTIDNDLIGTDYTIGYDTAINTVMEAVDKIRDTAES
                     HNRLFFVEVMGRDAGLIALRSGIAVGAEDIFIPETKTDLDRLINRLAACRKDKSSKII
                     IVAEGDEAGGAFEIADKVKKALPEYDTRVTILGHIQRGGNPTCMDRVNSSRMGFAAVE
                     ALMAGKRNVMIGIMNNKVTHTPFENATKHLQEIYPDYLRMMEILSL"
     gene            4412..>4781
                     /locus_tag="PK610_08885"
     CDS             4412..>4781
                     /locus_tag="PK610_08885"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRJ36091.1"
                     /translation="MSGSERRTLFADVILPLAVPQYYTYRIPLEMNEDVLPGMRAIVQ
                     FGKNKLYTAIVRNVHQTAPRHYEAKYLDGILDERPIVTEQQMTFWEWMADYYCCTPGE
                     VFLAAVPGSLRLGSETVIVPD"
BASE COUNT         1321 a          986 c         1050 g         1424 t
ORIGIN      
        1 cagaataagt gcaagcgcag gagcttcaaa tacgttaatt aatatgtatt gggtattcgt
       61 tagtttcgat aaaacatccc gtaccaaata gacccaatat tgtttgatct tgttgggaat
      121 cttaaatgtg ctttccggaa tatctttttg cgcaggtgct tccggtaatt gattgccgat
      181 tttttcggtg tacaactcat tccactcttt tggagatact ttccggttct gagtggcatt
      241 tccgtattcg tcgactacct tactctcaat gatgttgaat atctgttccg ggtttacatt
      301 accacaggtt ggacattcac tttcattaca gtttacatga ttcaccagtg tcttgaaata
      361 gatcactgca tcaacaggat ttccgtaata aatagggaat ccgccaacat ccagaatcag
      421 aagacggtcg aacatcttaa agatatcaga cgatggctgg tgaattacca caaagaccaa
      481 cttacctttt aaagcaagct cttttaataa gtccatgatg ttttcagaat cccttgagga
      541 taatcctgaa gttggttcat ccacaaacaa taccgccggt tcgcggatca attcaagacc
      601 gatattcaag cgcttccgtt gtcctccgga aatggtttta tccaatggat ttccgacacg
      661 gagatctttt gtttcgtata gaccaagggc tgtaagaaga ttcaatacca gcttggtgat
      721 ctgtgcatca ttgaggtttc caaaacacaa tttggcattg taaaacaggt tctggaaaac
      781 agtcaactct tcaatcagaa ggtcatcctg agaaacgtaa ccaattacac cttcaatttt
      841 tttgctttca ctgtggatat taactccgtt gatggtaatc gcacctcttg taggagcata
      901 attaccattt aatacattca gcaaagtcga ttttcctgca ccggaacctc ccataattcc
      961 tagcagtttc cctgattctt ctgaaaaggt taatggatgc aatccgattt tccctccttt
     1021 aaagtggaac atgatctcgt ccaccttaaa tacgattttg ctgacggacg tatcgctgag
     1081 gaatttgcta atgatatccg aataataaat cggctgaaca cgcgatgaac ggatggaaga
     1141 accttgtgtg agaatgtaaa cccttagcgg tgtaatgttt attccattca ggtagatctc
     1201 cgaattcccg taataacgca ggaaataaat tcccaatgaa ggaatacgca atacgcgaat
     1261 gtgcccctga attccctcac tgtaaatatg gtgagcttct ttaaaaggat tctcttttga
     1321 attattgatg taaagcaggt aagctccatc ttccgtctgg tcttctttat tgtcaacgaa
     1381 atttttgcag cgacggaatt cttcttctga gatattgaac gtttcagcaa cagtactggc
     1441 aaactcaagc tcttgttcgg ttacagattg gttttcgttg atgaactcaa ggatcctgac
     1501 caatacaatg attttctgct tttgggcaag ttcctcattg atctgggtac aaatccgcag
     1561 aacttttaca gaatttacgg atgtacgttt ttttgcaccg tcttttttct gcgatttccc
     1621 ctggtgagct tccaggtatt cgtcaaatag acgaagatat tcgtccacca actcctggtt
     1681 aagttgcgac ttcaggaaga tctcaacaat gcggcgtcca cctccatcag ttgatcctga
     1741 ttcggcttcg ggtccgtcct catcttcaac cctcgcgatg atggcgaaaa gttgcataag
     1801 ggcttttaat atccgttcac tcatgctgtg cgtggtgttg tctcaaacac gaaaagacat
     1861 ttactgcgaa atgtcttttc agatagattg agaatgaact tttacttttt caattgctta
     1921 aacccaatga gcattacggt acaaccggaa ctctttttat tgggatcaag acgtgcttca
     1981 atgatgacat ctactgttga ttcaactttg aaatcccagt agggtgcgtt tccataatcg
     2041 gcattcgaaa aaagaagatt gcgctcctta tcgtatacct caaaaatgac ataattatct
     2101 ttcgtacctg cagatgtagc aatgcgataa gttgaaccgc cataaaatgt agtggtaaac
     2161 tcagcagtct gctcttcgtc caataaggca cggtaagtct gaccatcaga gatgaagatt
     2221 gagcctttat ccttttttgc aggcttggca aggtgacctt tacaaacatt ggtaagtgaa
     2281 tcacagcttt gagctccagc attgcctgaa aacgcggcac tgaagagtac tataacggaa
     2341 aggactaaag ttctcatagt tcagattgta caattttggt acggagttct tttactttaa
     2401 gggtaatgtt attcaggtta tcaggagtga tgtccacctt ggtagaactc ttgattacgg
     2461 taacctgatt ctcagcgtcc gtttcaggct tctgataggt ataggttact ttgatctggt
     2521 cgaacaaagt ataaagtgat tgcatttctc caataaggtt tgcaatgctt tcttcagtat
     2581 tgtactgttc aagaagttca ataagatttc caagtgtggt tttttgctca ccaatacggt
     2641 tgatgatttt ctgatctttg gttttgttta ccacttcaca ggcaaaatac aatgcttcaa
     2701 tccatcctcc tgttaataca agtcctgctt cctgggcacg atcgttggtc ttcaggtatt
     2761 cgtcaccatt tcggtaagcg tcacttacaa ttaccagcat ggaatctttg ttgcccatgt
     2821 tttctgaaaa acgatcgatg atgtcttcag aaaatgcccc acccatatcg agttctttag
     2881 aaagctgctc aacagaagag aggtacgaca ttgcatcggt ggtattatcg tacatggttg
     2941 cgtaaccaag atcagctccg taaactccga ggttcaatgc cttattgaac ttagtggaat
     3001 agttggctgt ttgatctgta gaattgagca tggctgcatt gtaaggcgcg cctgtgcttt
     3061 taatcaattc tgcggtttgg aacggagatg gaatactgaa taaacgtccg ttgatagaga
     3121 tgcccattgc atcacttgtg tctaatacct tgggatcgac tttcgtcaca tcctgttcat
     3181 tggtattgga gtcgccgcaa gcggaaaaca tgagcatgga gactcccagc gcgataaaag
     3241 aaattgaggt gatccctctg gttcggttct gcataatgtt caggagtaga attgaacggt
     3301 gcaagtaaaa aaaaatagtt gaattgacaa agcggaaacg ttcgataaaa aaaatgaaac
     3361 tgtcgcccaa ctggggcatt atggggttaa atccgatggc ttttatccct ttcttccgta
     3421 ttttgcgcct atgaaaaaga tcgcagtttt tacctcagga ggcgattcgc cgggaatgaa
     3481 tgcctgtatc cgggctgttg tacggacagg tatctaccat gatcttgagg tagttggcat
     3541 cctccagggg tatgagggaa tgattgatgg agactttatt ccgatggatc gtaagtcggt
     3601 ggccaacatc atcaaccgcg gcgggaccat cctcaaaacg gcaagaagtc cgcgctttct
     3661 ggaacctgag ggacgaaaag cagcttatca acaattgaag aaggccggca ttgaaggggt
     3721 aatagccatt ggtggtgacg gaacctttac gggtgcacgg gtttttagtg agatttgtga
     3781 gattcctatt gttggatgcc ccggaaccat tgataatgat ttgattggaa cggattatac
     3841 cattggttac gatactgcta ttaacactgt tatggaggct gttgataaaa ttcgggatac
     3901 cgctgaaagt cataaccgct tgttttttgt ggaagtgatg ggaagggatg ccggattgat
     3961 agcccttcgg agcggaattg ctgttggagc cgaggatatt ttcatcccgg aaacaaaaac
     4021 cgatctcgat cgcttaatta accggcttgc agcatgcagg aaagacaaaa gttcgaaaat
     4081 aattattgtc gcagaaggtg atgaagcagg aggtgcattc gaaattgcgg ataaagtgaa
     4141 aaaggcgctt cccgaatacg atacacgcgt taccattctc ggtcatatcc aaaggggagg
     4201 taatccaacg tgtatggacc gcgtcaattc ctcacgtatg ggatttgctg ctgttgaagc
     4261 tttaatggca ggaaaacgaa acgtaatgat tgggataatg aacaacaaag tgacacatac
     4321 cccatttgaa aatgcgacca aacacttgca ggaaatctat cccgattatc ttcgcatgat
     4381 ggaaattcta tcgctttaat cactttttat tgtgtccggt tccgaacgac gtactttatt
     4441 tgctgacgta attctgcctt tggcggtacc tcagtattat acgtatcgca taccactgga
     4501 aatgaacgaa gatgttcttc ccggcatgcg cgcaattgtt cagttcggga aaaataaatt
     4561 gtacaccgcc attgtaagaa acgttcacca aacagcaccc cgacactacg aagcgaaata
     4621 tttagacggc atacttgatg agcgtccaat cgttacggag caacaaatga ctttttggga
     4681 atggatggcc gattactatt gctgtacgcc cggagaggtt tttcttgcag cagttcctgg
     4741 ctcactccga ttgggaagtg aaacggtaat tgttccggat c
//
LOCUS       DAOMYG010000254         4772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6223_length_4772_cov_10.736697, whole genome shotgun sequence.
ACCESSION   DAOMYG010000254 DAOMYG010000000
VERSION     DAOMYG010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6223_length_4772_cov_10.736697"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1270)
                     /locus_tag="PK610_08890"
     CDS             complement(<1..1270)
                     /locus_tag="PK610_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36092.1"
                     /translation="MKNNLLLLLLCLTGFNAGAQSIFFQKIIGNSGSFYSRDVIQTAD
                     TGYVVCGSSSGLGDYTANGFLMKLDKFGTRLWTKSYGGSNADWAMRVLEESDGSLVFA
                     GYTNSTNNSGYDIWLAKTNSNGVLQWSRTYGGSDWDFGYSILKTNDGNYLIAGETYSF
                     GNGNADGFLLKVDMNGDSLWMQTYGGPLNENARKVITLPDGSFIFTGTTNSPPSTSKD
                     IWLVKTDAMGAVVWDRRLGSAADDEGQSIVLSRTNGEIVGVGSTDSSYSTLNSDQYYF
                     RTDDQGMLICHFLYGGPAEETTHDVCERWNGYGIVAGMTLSYGSGFGDMICYYPHNGC
                     FWTGGQTIGTPDRDEAWAVKETSDSGYVVVGQTENGPGFTNVYIVKAGPTMFVTPLLI
                     TEFDISSVDEVATTNSLEVYPNPFNGPINIS"
     gene            complement(1270..2526)
                     /locus_tag="PK610_08895"
     CDS             complement(1270..2526)
                     /locus_tag="PK610_08895"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HRJ36093.1"
                     /translation="MKSRIIYTLLVHLCLIPAVHAQSVVEPEFDPQEGTAHEVKLFGN
                     GYYLSSGTPIRFMSKFTKGGFIDSTLKSDVLSDLRKNNRIGAEIHTGVEYYAHRIPFF
                     KSSGWSWYFGASYHMNYNAAYSKNLFQLAFFGNRGFAGQTVELGPLNANVLSYGKLSA
                     GAFNKETKSRIALSLVAGQQFSQLNVDRALFYTAGNGSQLDLDLSEQFRSSDTSHRTG
                     KINGWGSAADFVLYFNTGSNKSLKINQGFRVALEDFGFIRWNANSITQKKDSAYRYEG
                     FEVTDLLNGINQPFNVDNLNDTLGIKGTTGAQTHMLPLTFSVAAITETFSDKKVQAFY
                     GFRLRALSNYTPMIHLGMQFNPIHDLALSLYGAFGGYGGFRAGLSASGKIGEHIRYGF
                     CTGNIAGWISKNAYGRDLSLTISTRF"
     gene            complement(2523..4112)
                     /locus_tag="PK610_08900"
     CDS             complement(2523..4112)
                     /locus_tag="PK610_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36094.1"
                     /translation="MKKSFLLLLPALSMVWGLSSCKRDLQRPSWDIGVVGPVIHSSLG
                     LGNMIADSLLQVNPDSSVVLSFSGTVYELDADTLLKLPDTTITNSFSLPTIIPSVNVT
                     PGQLVFNQTETRYFNGQGVQLTIADLRSGGCYLTLSSTINQPVDFTYEILTAFKSGQH
                     FITTSRIPPGSIAQPSQVVVYVDLSGYRFILTDAGATQFNQYTTKTTIKMSADAQPTT
                     VTNQDVVAVSSTFSGLEPSYAKGYFGSRIESFVNEPSNFSSFEKIISGVLDIDQVNVN
                     LTFTNYIGADARITLQSLKAARGADTVVLAHSIIGSAININRATDPGGIVIPSTYSVN
                     LNNGNSNIDQIIELLPLAFSSSLDFHINPLGNVAAHHDFFYAPKTVKADLNFTLPLNI
                     IANNLTLADTIDLNLERGKNGHIRSGKLHIDISNGFPLAADFQLFFMNGSGTLTDSIV
                     QPNSILAAYTNAQNIVTANRDSELIITVNESQMDRLYNGERLLLRIAFSTSSLTQHVT
                     LYDYYRIHVRMRGDFNYRIGS"
     gene            4438..4692
                     /locus_tag="PK610_08905"
     CDS             4438..4692
                     /locus_tag="PK610_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36095.1"
                     /translation="MLNVLILNIEFNENHFLKRALLGGALGFFIYLIVLTLGISYHTR
                     GFEHLVIDFSWQMIEQAFGGMIVNLCLNIYRRMDAIEGLE"
BASE COUNT         1319 a          997 c         1034 g         1422 t
ORIGIN      
        1 acgaaatgtt gattggtcca ttaaaagggt tgggataaac ttcaagagaa tttgttgttg
       61 ccacttcatc aaccgatgaa atgtcaaatt ctgtaattag taatggtgta acaaacatgg
      121 tgggacctgc ttttacgata tacacattgg taaagccggg gccattttca gtttgaccga
      181 ccactacata tccgctatct gatgtttcct tcactgccca ggcttcgtct ctgtccggtg
      241 tgcctatcgt ttgtccgcct gtccaaaaac aaccgttgtg cggatagtaa cagatcatat
      301 cgccaaatcc gctgccgtac gataatgtca ttcctgcaac aatgccataa ccattccacc
      361 gttcgcaaac atcatgtgtt gtttcttcgg ccggaccacc gtacaaaaaa tggcaaatca
      421 acattccctg atcgtcggtt cggaaataat actgatcgga attgagtgtg ctgtaggatg
      481 agtccgtact tcctacacca acaatttctc cgtttgtgcg ggatagtaca atggattgac
      541 cttcgtcatc agcggccgat ccaagtctgc gatcccatac cacagctccc attgcatccg
      601 tttttaccag ccagatatct tttgaagtgc tgggcgggct gtttgttgtt cctgtgaaga
      661 tgaatgaacc gtcgggtaac gtaataactt ttcttgcgtt ttcatttaat ggtcctccgt
      721 aggtttgcat ccagagggag tcgccgttca tatctacctt aagcagaaat ccatctgcgt
      781 tgccattgcc aaaactgtaa gtttctcctg caataagata attaccatca ttcgttttta
      841 aaatactgta tccaaaatcc caatccgatc cgccatatgt tcgcgaccac tgtaaaacac
      901 cgttgctgtt cgtttttgcc aaccagatgt cgtaacctga attgttggtg ctgttggtgt
      961 atcctgcaaa taccaaactg ccatcactct cttcgagcac acgcatagcc caatctgcat
     1021 ttgaaccgcc gtatgatttg gtccataagc gtgtcccgaa tttgtcaagc ttcatcaaaa
     1081 aaccgttcgc tgtataatca ccaagtccac tgcttgatcc gcaaactaca tatccggtat
     1141 ctgctgtttg aataacatct ctcgaataaa atgaacctga attccctatg atcttctgaa
     1201 agaaaataga ttgcgccccg gcgttgaatc cggttaaaca caatagtagg agaagtaaat
     1261 tatttttcat caaaaacgag tactaatagt taaactgaga tcacgaccgt aagcattctt
     1321 actgatccag cctgcaatat tgcctgtgca aaaaccataa cgaatatgtt ctccgatttt
     1381 tccggatgca cttaagcctg cccggaaacc accgtatcct ccaaatgcac catatagtga
     1441 aagtgcgaga tcgtgaatgg gattgaactg cataccaagg tgaatcattg gtgtgtaatt
     1501 ggataaggct cgtaaacgaa aaccataaaa ggcctggact tttttatcac tgaatgtttc
     1561 tgtaatggca gccacagaaa atgttaaggg cagcatatgc gtttgtgctc ctgttgttcc
     1621 tttaatgccc aaagtgtcat tcagattatc tacgttgaaa ggctggttga ttccattcaa
     1681 caggtcagta acctcaaatc cttcgtagcg gtaagcgctg tctttttttt gtgttatgct
     1741 atttgcattc caacggataa acccaaagtc ttccaaagca accctgaatc cctgattgat
     1801 ttttaaactt ttattgcttc ccgtattgaa atacaataca aaatccgctg cgcttcccca
     1861 tccgtttatt tttcctgttc gatgtgaggt gtctgaactt cggaattgtt cactgagatc
     1921 aagatcgagc tgcgaaccgt ttccggctgt atagaataag gcgcggtcta cattgagttg
     1981 actgaattgc tggcctgcaa ccaaggaaag tgctatgcgt gattttgttt ctttgttaaa
     2041 ggcacctgca gacaattttc cataactgag cacatttgcg ttgagtggac caagttctac
     2101 ggtttgtcca gcaaaacccc gattgccaaa aaatgcgagt tgaaaaagat ttttactgta
     2161 agctgcattg tagttcatgt gataggacgc cccgaagtac cacgaccaac ctgagctttt
     2221 aaaaaacgga atacggtgag cgtagtattc cacgccggtg tgtatttctg ctcctattcg
     2281 gttattctta cgaagatctg aaagaacatc tgacttaagc gtagaatcaa taaagccacc
     2341 ttttgtgaac ttcgacataa aacggatagg tgttccgctg ctgaggtaat atccgtttcc
     2401 aaatagtttt acttcatgcg ctgttccttc ctgtggatcg aactccggct ctacaacaga
     2461 ttgagcatgt actgccggaa ttaagcagag gtgtactagc aaggtgtaaa ttattctgct
     2521 tttcatgatc cgattctgta attgaagtct cctcgcatac gaacatggat gcggtaataa
     2581 tcgtaaagtg ttacatgctg agtgagcgaa ctggtagaaa acgcgattct taataacaaa
     2641 cgttcgccat tgtataaacg atccatctgc gattcattta cggtaataat aagctcactg
     2701 tcgcgattag cagtaactat gttttgtgcg ttggtatatg cagcaagaat ggaattaggt
     2761 tgtacaattg aatcggtaag agttcctgaa ccattcataa aaaacaattg aaaatctgca
     2821 gcgagtggaa aaccattgct gatatcaata tgtaattttc ctgagcggat atgaccattt
     2881 tttcctcgct ccagattgag atcgatggtg tcggcaagtg taagattatt ggcgataata
     2941 ttaagtggca acgtgaaatt caaatcagct ttaactgttt ttggtgcgta gaagaaatca
     3001 tgatgcgctg caacgttccc aagtggatta atatgaaaat cgagactgga tgaaaatgca
     3061 agtggtaaca gttctatgat ctgatcaata ttcgaatttc cgttatttaa attcacactg
     3121 tatgtacttg gaataactat tcctccggga tcggttgcgc ggttgatgtt aatagcgctg
     3181 ccaatgatgg aatgagccaa tactactgtg tccgctcctc ttgcagcttt taatgattgt
     3241 aaagttattc tggcatctgc tccgatgtaa ttggtgaacg taagatttac atttacctgg
     3301 tcgatgtcca atacacctga aattattttt tcaaaagatg aaaaattgct gggttcgttt
     3361 acaaaacttt cgatgcgtga tccaaagtac cccttggcat acgatggctc cagtccgcta
     3421 aacgtggaac tgaccgcaac tacatcctgg ttagtgactg ttgttggctg tgcatcagcc
     3481 gacattttga tggtggtttt ggtggtgtac tgattaaatt gggtagcacc tgcatcggtg
     3541 agaataaaac ggtaaccact caaatccaca tacactacta cctgactggg ctgagcaata
     3601 ctacctggtg gaatgcgtga agttgtgata aaatgctgac ctgatttaaa agcggtaaga
     3661 atttcatagg tgaaatcaac cggttggtta atcgtgctgg ataaggtcaa ataacatcca
     3721 cctgagcgta gatctgcaat ggttaattga acgccctgtc cgttgaaata gcgggtttct
     3781 gtctgattaa aaacaagctg acccggagtg acattcacag agggaatgat ggtaggcaga
     3841 ctgaagctgt tggtgatggt ggtatccggt aactttagca gcgtatctgc atccagttca
     3901 tatactgtac cgctgaacga aagtacaaca gagctgtcag ggttaacctg caaaaggcta
     3961 tcggcaatca tgtttcctaa tcctaaagag gaatggatta ccggcccaac aacaccaata
     4021 tcccaggagg ggcgctgcag atcgcgctta caggagctga gcccccacac catggagagg
     4081 gcaggcaaca ggaggagaaa ggactttttc atctatgcct aaatgtacaa aaatcccttt
     4141 cccattgatt ttggaatgag ttttcggttg gtataaaccg gaaaacagca ggacttggtc
     4201 ccggaaattg acttttctgc gtaaaaatgg ttgaaaattg cttgaacaag gtaattttgc
     4261 gccatgtttg gattaaccct caggcggttt cttatttcat ggttattgtc gtccggatta
     4321 atgatcatgc tttctctgtt atggcacggg gtaattttaa atgatttacg gaacctgcct
     4381 ttccacggga gtttttttct agtgctcacg ggtttggctt accttgctat tgggtttttg
     4441 ctgaacgttt taattctgaa tatcgaattc aacgaaaacc acttcctcaa acgtgcattg
     4501 ttgggaggtg ctttgggatt tttcatttac ctcattgtcc ttactctcgg aatatcctac
     4561 catacgcggg gatttgaaca tctggtcatc gatttttcat ggcagatgat cgagcaggct
     4621 tttggtggta tgatagtaaa tctgtgcctg aatatttaca ggagaatgga tgcaatcgaa
     4681 ggtctagaat aacaggtttt cattcgggaa aagtactaaa cccaatgtgg tttaaatgct
     4741 gaaattttaa ttgatttgaa ataggggaag ag
//
LOCUS       DAOMYG010000255         4730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6298_length_4730_cov_7.939679, whole genome shotgun sequence.
ACCESSION   DAOMYG010000255 DAOMYG010000000
VERSION     DAOMYG010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6298_length_4730_cov_7.939679"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1063
                     /locus_tag="PK610_08910"
     CDS             92..1063
                     /locus_tag="PK610_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRJ36096.1"
                     /translation="MKRNFLKYCSIMILGIVLHNDVRAQDPEFTQFFANPLYLNPAFA
                     GSKRCPRINLNYRNQWPALSGTFVTTSASYDQHVESIYGGLGLLVTNDKAGEGTLNTT
                     TINAMYAYQLKLGRKFSLRFGVQASYFQKSLDWSKLTFGDMIDPRRGFVYQTADVPRG
                     GSVGNMDFSAGILGFSENFYIGFSAHHLTEPNESLVTGTSPLPMKLSGHAGAVIPLGN
                     SKYSTNDATLSPNIMYRSQGTFQQLNMGLYVNTKNIVFGAWYRNKDAFIALIGVKSDI
                     FNFGYSYDVTVSKLTNASGGSHEISMAINIPCKPKKRQFRTISCPSF"
     gene            complement(1097..2755)
                     /locus_tag="PK610_08915"
     CDS             complement(1097..2755)
                     /locus_tag="PK610_08915"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019039190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRJ36097.1"
                     /translation="MKNHFKTIFLSFSAILLVQSCAHKAEDADLIVHNAHIYSLDETN
                     TVYEAMAIKDGKIIELGPERQILNKYRSKEQFDAGKNYIYPGFIDAHGHLLGYGQLML
                     QCDLTGTNSLEEVLSKVEKFGAKSSSAVVYGRGWDQNDWQNKQFPDNVKLSQLFPDRP
                     VVLSRIDGHAVLVNNKALELAGVDASTSVEGGDFIKNADGKLTGVLIDNAIERVLGSL
                     PVQTDDEIRRALETADRDLVSYGITTVSDAGVDKSVVDVMHALMKDGSFKLRVYAMMR
                     PTAANLEYYSVNGPYKDDRLNIRSFKFMADGALGSWGACLKEPYHDAALPGHRGKMTV
                     SLDELRDAADLLNESGFQVNTHCIGDSANKAVLSIYAEILGDMNDKRWRIEHAQILDT
                     ADINYFFRYSIIPSVQPTHAISDMPWAENRIGASRMIGAYAYRTLLNTNGWIALGTDF
                     PVEHPDPLMTFFAAVFRKDRKGNPADGFLINERLSREEALRGMTIWAAKSNFEEDEKG
                     SLEKGKFADFVVLNQDLLNVREKDFFKTQVNATYINGERVYSYK"
     gene            complement(2793..3551)
                     /locus_tag="PK610_08920"
     CDS             complement(2793..3551)
                     /locus_tag="PK610_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ36098.1"
                     /translation="MKTILITGATSGFGKAIATLFASNGWKIIITGRREERLLNLQEA
                     LRKKYGVEVLALNFDIRNRDLTLNELSNLPKEFANVDVLVNNAGLALGLSSIDEGDVD
                     EWEQMIDTNVKGMLYAIRAIAPGMRARKSGHIINIGSTAGKEAYKNGNVYCATKHAVE
                     AITKGTRADLLPYNVKVTSVCPGMAETEFSEVRFSGDTNRAKQVYQGFEPLRPEDIAE
                     VVYFAATRPAHVCLNDVILTPTAQANSFYTERNN"
     gene            complement(3586..3780)
                     /locus_tag="PK610_08925"
     CDS             complement(3586..3780)
                     /locus_tag="PK610_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36099.1"
                     /translation="MQRLFFFIVFFSSQLLNAQDVNVPVEEKGFLGLDEFQVMNLIYL
                     VSVLVVILFMRRMRNRNYKE"
     gene            complement(3838..>4730)
                     /locus_tag="PK610_08930"
     CDS             complement(3838..>4730)
                     /locus_tag="PK610_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ36100.1"
                     /translation="QGQWLAPINMGYPINSPYDDFICAMSANSKYMYMASNRPGGKGG
                     LDLYKVTFWGPEKKLIVDTEDYLLSSIASPIQDNVIEASVQVQKKSLTVFKGRTIDAI
                     TKKPVEAEIEIVDNATGQGVSVFNTNSATGKFLLSLNSGKNYGIAVKAPGYLFHSENF
                     DIPLYSDYNLVDKEIELKNIAVGSKIALRNIFFATGSATLTKESNSELDRLVKLLKDV
                     PKLKIEISGHTDNVGSESMNQKLSEDRAASVVAYVISKGISKDRLTSKGYGSTRPVAS
                     NNSADGRQQNRRTEFEILSN"
BASE COUNT         1303 a         1059 c          950 g         1418 t
ORIGIN      
        1 cacatgaagt ttccatgcgc ctttctctga atggtcttgg taaaaaacaa agtgtgatcc
       61 ttaacagtcc taaataagaa aggaaagagt tatgaaacga aatttcctca aatactgtag
      121 cattatgatt ctgggaatcg ttctgcacaa tgatgttcgc gcacaggatc ctgagttcac
      181 tcagttcttt gcaaacccgc tttatctgaa tccggcgttt gcaggttcga agcgttgtcc
      241 gcgcatcaac ctcaactacc gtaaccaatg gccagccttg tcgggaacat ttgttactac
      301 atctgcatcg tatgaccagc atgtggaatc aatctatggt ggactgggtt tgttggtgac
      361 caacgataaa gcaggtgaag gaacgttgaa cacaactacc atcaacgcga tgtatgccta
      421 tcagcttaag ctgggacgca aattttcact gcgctttggt gttcaggcat cctatttcca
      481 gaaatcgctc gactggagca aactcacctt tggtgatatg atcgatccgc gaagaggttt
      541 cgtttatcaa actgctgacg taccgcgcgg cggctcagta ggaaacatgg atttctctgc
      601 aggtattctc ggtttcagcg aaaactttta catcggtttc tctgcacacc atttaacaga
      661 gcccaacgaa tcgctggtaa caggtacaag tccgctacca atgaaacttt cgggtcatgc
      721 cggtgcagtt attccattgg ggaattcgaa atactctacc aacgacgcaa cgctatctcc
      781 caatatcatg taccgttcgc agggaacatt ccagcaattg aatatgggtc tttatgtcaa
      841 cacaaaaaac attgtttttg gtgcatggta ccgaaataag gatgcgttta ttgcgttaat
      901 aggtgtgaaa tccgacatct tcaattttgg atacagctat gatgtaaccg tttccaagtt
      961 gaccaatgca tctggtggat cacacgaaat ttccatggcg atcaatattc catgtaaacc
     1021 taaaaagaga cagttcagga ccatcagttg tccttcgttc taaagacgct ttatttggtg
     1081 ttacggtgag cgggttttac ttatacgagt agacccgttc gccgtttata taggttgcat
     1141 ttacctgagt cttaaaaaaa tccttctccc gtacgttaag caagtcctga ttgagcacaa
     1201 caaaatctgc aaactttccc ttttcaagtg agcctttttc atcctcctca aaatttgatt
     1261 ttgctgccca aatggtcatt cctctgagtg cttcttcgcg ggataatcgt tcattgatca
     1321 aaaatccatc agcaggattt cctttgcgat cctttctgaa aactgctgca aaaaatgtca
     1381 tcagcggatc aggatgctca acgggaaaat ctgttccgag tgcaatccat ccgtttgtat
     1441 ttaacagggt acggtaggca taagcgccga tcatcctgga agcaccgatt ctgttttctg
     1501 cccagggcat atcagaaatt gcatgtgtgg gttgaacgga aggaattata ctatacctga
     1561 aaaaataatt gatatcggct gtatccaaaa tttgtgcatg ttcaattctc cagcgcttat
     1621 cattcatatc cccaagtatt tcggcataga tacttaatac cgctttgttg gcagaatcgc
     1681 caatgcaatg tgtgttcacc tgaaaaccac tttcgttgag caaatcggca gcatcacgta
     1741 attcatcaag actcactgtc attttacctc gatgtccggg tagtgctgca tcatggtagg
     1801 gttctttcaa acacgcaccc catgatccca atgcgccatc ggccataaat ttaaatgagc
     1861 gaatattcag acgatcatcc ttatacggac catttaccga ataatattca aggtttgcag
     1921 cagttggacg catcatggca tacacacgta gtttaaatga tccgtccttc atcaatgcat
     1981 gcatcacatc gactaccgat ttatctactc cggcatcgga tacagtagta attccataac
     2041 tgactagatc acgatctgca gtttccaatg cccgacgtat ttcatcatcg gtttgaacag
     2101 gtaatgaacc aagaacacgt tcaattgcat tatcaatcag cactcctgta agttttccat
     2161 ctgcattttt tataaaatct cctccttcta cagaagtgct tgcatcaact ccggctaatt
     2221 cgagtgcctt gttgtttacc aatacagcat gaccgtcaat acgactcaat acaacagggc
     2281 gatcaggaaa tagttgacta agttttacat tatcgggaaa ttgtttgttc tgccaatcgt
     2341 tttggtccca tcctctacca tatactactg cagaagaaga ttttgcaccg aacttttcga
     2401 cttttgagag tacctcctcc aatgaatttg tccctgtaag atcgcattgc agcatcaact
     2461 gtccataacc cagcagatgt ccatgcgcat cgatgaagcc cgggtaaata taatttttac
     2521 ctgcatcgaa ctgttctttg cttctgtatt tattcaggat ctggcgttca ggccccagtt
     2581 caatgatttt accatctttg atagccatcg cttcataaac ggtgtttgtt tcatccagcg
     2641 aataaatatg cgcgttatga acaatcagat ccgcgtcttc tgccttatgc gcgcaggatt
     2701 gcacaagcag gattgcacta aaagaaagga aaattgtttt gaaatgattt ttcatgactg
     2761 aaaacaggat cgtacaaacg gtttgtattt cattaattat ttcgctctgt ataaaatgaa
     2821 tttgcctgag cagttggagt tagaattaca tcattcagac aaacatgtgc aggtcgggtg
     2881 gcagcaaaat aaacgacctc cgcaatatct tcaggacgca atggttcaaa accctgataa
     2941 acctgttttg ctctgttggt atcacccgaa aaacgaactt cggaaaactc tgtttcagcc
     3001 ataccgggac aaacactggt tacctttaca ttatatggca aaagatctgc tcttgtgccc
     3061 ttggtaattg cttcaactgc atgtttggtt gcgcaataca catttccatt tttatacgct
     3121 tcttttccgg cagtggaacc gatgttgatg atatgtccgc ttttgcgtgc acgcattccg
     3181 ggtgcgatgg ctctgatggc atatagcatt ccctttacat tggtatcaat catttgttcc
     3241 cattcatcca catcgccttc gtcgattgaa ctcaaaccaa gtgctaatcc cgcattgttt
     3301 accaatacat caacgttcgc aaactccttt ggaaggttcg acaattcatt aagggttaga
     3361 tcgcggtttc tgatatcaaa attaagtgcg aggacttcca ctccgtattt ttttctcaac
     3421 gcttcctgca aattgagcaa tcgttcttcc cggcgcccgg taatgatgat tttccagcca
     3481 ttggaagcaa aaagagttgc aatcgccttt ccgaatccgg atgtggcacc tgtaattagg
     3541 attgttttca tgaacgtgct aattttttaa ctgaacaatt cgggattact ctttgtaatt
     3601 gcggttacgc attcggcgca taaacagaat cactaccaat acggaaacca ggtaaatcag
     3661 attcattacc tgaaattcat caagtcccaa aaaacctttt tcttctacag gaacatttac
     3721 atcctgagcg tttaacaact gtgaagaaaa gaatacaata aagaaaaata gtctttgcat
     3781 agtggtaaaa aaaagtcccg acgtaaatgt cgggactttc tatttgtgaa gtacaaatta
     3841 attggagaga atttcaaatt ccgtgcgtcg gttttgctga cgtccatccg ctgaattgtt
     3901 ggatgcaaca gggcgtgtag atccataacc tttggaagta aggcgatctt tggagattcc
     3961 cttggaaatc acataagcta ccactgaagc agcacgatct tccgaaagtt tctggttcat
     4021 gctttcagaa ccaacattat cggtatgacc ggagatctcg attttcagtt tgggaacatc
     4081 cttcagaagt tttaccagac gatcaagctc tgaattggat tccttggtaa gtgttgccga
     4141 acctgtagcg aagaaaatgt tacggagtgc tattttgctt cctacggcaa tattcttcaa
     4201 ctcaatttct ttgtccacaa ggttataatc ggaataaagt ggaatatcga agttctcaga
     4261 gtggaacaaa tatcccggag ctttaacggc gataccgtag ttttttccgc tgtttaatga
     4321 aagtaggaat tttccggttg cactgttggt attgaaaaca gatactcctt gtcctgttgc
     4381 attgtcaacg atctcaatct ctgcctctac cggtttcttg gtaatggcat cgatggttcg
     4441 tcctttgaaa acagtcagac ttttcttctg cacctgaaca gatgcttcaa tcacgttatc
     4501 ctgaatcgga cttgcaatgg atgagagaag ataatcttcg gtatcaacta tcaacttctt
     4561 ttccggtccc cagaaagtga ctttgtagag atctaatccg cctttaccac ctggacgatt
     4621 ggaggccatg tacatgtatt tgctgttggc tgacattgcg cagatgaaat catcgtatgg
     4681 cgaattgatt ggatagccca tattgatcgg agcaagccat tgtccttgct
//
LOCUS       DAOMYG010000256         4722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6313_length_4722_cov_9.223698, whole genome shotgun sequence.
ACCESSION   DAOMYG010000256 DAOMYG010000000
VERSION     DAOMYG010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6313_length_4722_cov_9.223698"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..702)
                     /locus_tag="PK610_08935"
     CDS             complement(13..702)
                     /locus_tag="PK610_08935"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ36101.1"
                     /translation="MLQPQIILQWDVRNWSKALRYWEQHVEWSQVNECLELGGREGGL
                     SLWLAEKGKTVVCSDLENTASTAGPQHEKFPFSKSISYRDIDATSIPFENKFDLIVFK
                     SILGGIGRNNDKKAQQAVFATAFKALKPGGVLLFAENLQASALHRYFRKRFTKWSAYW
                     RYPTFEEFEAFSNKFVDVQLNTNGFLGTFGRTEKQRHLLSFFDKLIFDHITPKKKRYI
                     IYGICTKPNQK"
     gene            complement(813..2201)
                     /locus_tag="PK610_08940"
     CDS             complement(813..2201)
                     /locus_tag="PK610_08940"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRJ36102.1"
                     /translation="MNKTLNPDSPLSRIKLFFTFFDSRWEDLTRENWKKTVYRDFSAG
                     LIVAMTAIPMAMGFAMAMGMRPEHGIIAGALACVIGRTFGGSKYQVYGPTAAFIPVIA
                     SLIAKYGPAGGGDFATAHGFLVLVSILSGIVLIVMGIYGFGRYAKHVPNSIIVGFTIG
                     IATAIALTNLEDILGIESFSDLLGQDEDIKGGILHNFYLASQNLDKINLWSIFLGALT
                     FGLTKLLLRISIFIPGPVIAMGTATILSATFLADKNIILVKDIYGTIPNNFFAFTAPT
                     LPGLNGSVILDILFFVVAIVFVSGVESILCSSMADKLAGNKKTPFNPDKEFFGQGMVQ
                     IFTPMVQGFPCTGALARTATSIKAGAQTPLAGYFKAVLKLTMAFFLAQYLELIPMACI
                     GGILVWVASNMIKPSEIKEIKHLGKFEFSMMVYTAVMVPLTDFLTGVLSALIIYFLVK
                     NVFKKKKSAPEA"
     gene            complement(2212..2688)
                     /locus_tag="PK610_08945"
     CDS             complement(2212..2688)
                     /locus_tag="PK610_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36103.1"
                     /translation="MPKTILIPTNFKVSSLYTLKVALEDYKEKEVEIILLYADVLESS
                     ITELLFFSPRKRIEELMSDEFRTAIEIIKNRFEPNLTTLSIQLFQGKNQASFVNFLEA
                     KCVDEIYTLSKPNLPAGTPCAKLHFFIDRATIPVKKVDHHNNDLLSEGDHLQSLFA"
     gene            complement(2780..4153)
                     /locus_tag="PK610_08950"
     CDS             complement(2780..4153)
                     /locus_tag="PK610_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRJ36104.1"
                     /translation="MKTPSKIALFLVITNVIILLMFGGGIYYFLNAYSYVDFYKRLET
                     RAGIAARYNLDQDELTADAYKKIREQHLEKLSEEKEYIIKINPQLSVGGISEESKLPA
                     ELLQEIIDKGKSTYKNGNTFYAGQTYPQGKANYIVVVSAENYYSSHHLSFLRNILIIG
                     LLLTLLVTSFTAYYFSRYIFEPIRQITQKVKTISAENFHLRLEEEENNHEIKELKLTF
                     NDLLNRLETAFETQKNFISNASHELGTPLTSIIGEADVSLIKNREPEEYQESLKSILK
                     QAERLDEIIKSLIFLAQTGYEGKRIPFETIRVDDILWAVKTVIDKLNPDNKIHFDFSL
                     LPDDPRKLKIQGNRQLLHLAFANLLNNACKYSYNKPVTVSIGSSNSEVVIIIRDNGVG
                     IPEDEIPFIYDPFFRASNTKYFEGYGIGLPLTRNILRLHQGTLQVTSVINSGTTVQVT
                     IPLSFPK"
     gene            complement(4150..>4722)
                     /locus_tag="PK610_08955"
     CDS             complement(4150..>4722)
                     /locus_tag="PK610_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ36105.1"
                     /translation="KLAREQVFQLIILDIMLPGKSGLEVCQNIRSFNTKTPILFLTAL
                     GTSENVALGLNTGADDYLIKPFKFIELSARIKALLRRSSGELISETTDHILEISDLTI
                     DDRAKQVFRNAKPITLTATEYRLLLTLAKNKGKVMSRSELLESAWDIHFNLGTNVVDV
                     YINYLRKKIDSESEIKLIHTVVGMGYVLKT"
BASE COUNT         1382 a          900 c          972 g         1468 t
ORIGIN      
        1 ccatagctct atttattttt gattgggttt ggtgcagatt ccgtaaatga tgtaacgctt
       61 tttcttagga gtgatgtggt caaaaatcag cttgtcaaaa aaggataaga ggtgacgctg
      121 tttttctgtc cttccaaatg ttccgaggaa gccgttagtg ttcagttgaa catcaacaaa
      181 tttattgctg aatgcttcaa attcttcaaa agtgggatat cgccaataag cagaccattt
      241 tgtaaatcgt tttctgaagt aacggtgcag agcacttgcc tgcaggtttt ctgcaaacaa
      301 caaaactccg ccgggtttga gtgctttgaa agcggtagcg aaaacagcct gctgtgcttt
      361 tttatcgttg ttacgaccaa ttcctccaag tatggattta aaaacgatga ggtcaaattt
      421 attttcaaaa ggaatggatg ttgcatcaat gtcccggtag gatatggatt ttgaaaacgg
      481 aaatttttca tgctgtggac ctgcagttga tgctgtattt tcaagatctg aacaaaccac
      541 tgtttttccc ttttcagcaa gccaaagcga taaaccgcct tcacgacctc ctagttcaag
      601 acattcgtta acctgcgacc actccacatg ctgctcccaa tagcgtaaag ccttcgacca
      661 gttgcggacg tcccactgaa ggattatttg cggttgcagc attgagcaaa gatagaagaa
      721 gtttttcaga ggccagttgg gtgcggtttt ttgcaacaaa aagaggcaga acgcattgtt
      781 ctgcctctga ataaattgat tgcgaatttt atttatgctt ccggagcaga ttttttcttc
      841 ttaaaaacgt tttttacaag aaaataaatg atcaatgcag ataatacccc tgtaaggaaa
      901 tcggttaatg gcaccataac tgcggtatat accatcattg aaaattcaaa tttaccgaga
      961 tgtttgatct cctttatttc cgatggcttg atcatattgc tcgcaaccca aaccagtatt
     1021 ccgccaatac aagccattgg tatcaattca aggtattgtg ccaggaaaaa tgccatagtc
     1081 agttttagca cagctttaaa gtaacccgcc aaaggagttt gtgcaccggc ttttatgctg
     1141 gttgctgttc gcgccaatgc tcctgtacat ggaaagccct gaaccattgg agtgaaaatc
     1201 tgtaccattc cctgaccgaa aaattcttta tccggattga atggtgtttt tttatttccc
     1261 gcaagtttgt cggccatcga tgaacagaga atgctttcta cacccgaaac aaaaacgatg
     1321 gcaacaacaa aaaataaaat atcaagaatt acgcttccat ttaatcccgg caaagtaggc
     1381 gcggtaaagg cgaaaaaatt gttgggaatt gtaccgtaaa tatctttcac aagaataatg
     1441 tttttatcgg cgagaaatgt tgctgaaaga atggtcgctg ttcccatggc gataaccggt
     1501 ccgggaataa aaatggaaat tctaagcaac agtttcgtta agccgaatgt aagcgctcca
     1561 aggaagatcg accagagatt gatcttgtct agattttgag atgccaggta aaaattgtgg
     1621 aggattcctc ctttaatatc ttcgtcctga ccaagtaagt cggaaaacga ttcaattccc
     1681 aaaatgtctt ccagattagt aagtgctatt gctgttgcaa ttcctatggt gaacccaacg
     1741 ataattgaat tagggacatg ttttgcgtat cttccaaatc cgtaaatccc cattacaata
     1801 agcacaattc cggatagaat ggagaccaga acaaggaatc catgtgcagt tgcaaaatca
     1861 cctccaccag caggaccgta ttttgcaatg agacttgcaa taaccggaat aaatgcagcg
     1921 gtaggaccgt atacctggta tttcgatcct ccaaacgttc tgccaattac acaggccaat
     1981 gcacctgcaa taattccgtg ttcgggacgc atacccatgg ccattgcaaa acccatagcc
     2041 atcggaatgg ctgtcattgc aacaatgagt ccggcactga aatcgcggta gacggttttt
     2101 ttccagtttt cccttgtcaa atcctcccaa cgggaatcaa aaaaggtaaa gaatagtttt
     2161 attctcgata atggagaatc gggattaagc gttttattca tggtttttgg tttaggcaaa
     2221 aagcgattga agatgatctc cctctgacaa caaatcgtta ttgtgatggt ctactttttt
     2281 taccggaata gtggctcgat cgataaagaa gtgtagcttg gcacaaggag tacctgcagg
     2341 gaggtttggt tttgaaagag tgtaaatctc atctacacat tttgcttcca gaaaattcac
     2401 gaatgatgct tggttttttc cctggaaaag ttgaatgctc aatgttgtca ggtttggctc
     2461 gaatcgattc ttaatgatct cgatggcagt tctaaattca tcactcatta attcttcgat
     2521 tctttttctt ggcgagaaaa acagaagttc agtgattgag ctttcaagaa catcagcata
     2581 cagaagtatt atctctactt ctttttcttt gtaatcttca agggcaacct ttaaggtgta
     2641 caaagaagaa actttgaaat ttgtaggaat cagaatggtc ttgggcataa gtataaaatg
     2701 tgcatttgtt atgaaatgca tagatcatac atgggaatta taatcggctt agagaaggat
     2761 tagaatttga ttagaattct tatttcggga aggaaagcgg aatagttacc tgaactgtag
     2821 tgcccgaatt tattacagat gtaacttgta atgtgccttg gtgaagacga agaatgtttc
     2881 gtgttaatgg aagtccgatt ccataacctt caaaatactt ggtgtttgag gcccggaaaa
     2941 atgggtcata gatgaaaggg atttcatcct caggaatacc aacgccattg tcgcgaataa
     3001 tgataacaac ttccgaattg ctggatccta ttgaaacagt tacaggtttg ttataggaat
     3061 atttgcacgc attgttcaac aagttggcaa aggccagatg aagcagttga cggtttccct
     3121 gtatttttag ttttcgggga tcatcgggca acaaactgaa atcgaaatga attttattgt
     3181 caggattcaa tttgtcgata accgttttta ctgcccataa tatatcgtca acccgaatag
     3241 tttcaaaagg aattcgcttg ccttcataac cggtttgagc aaggaaaatg agcgatttaa
     3301 taatttcatc caatcgctct gcttgtttta atatgctttt aagcgactcc tggtattctt
     3361 ccggttcccg gttttttata agcgatacgt ctgcttcacc aatgatggat gttaatggcg
     3421 tgcccagttc atgagaggcg ttactgatga agtttttttg agtttcaaag gctgtttcga
     3481 gcctgtttaa caaatcattg aatgttaact tgagctcttt aatttcatga ttgttttctt
     3541 cctcttccaa tcgcaaatga aaattttcgg cacttatcgt tttaactttt tgggtgattt
     3601 gacggattgg ttcaaatata tacctggaga aataataggc agtaaatgaa gttacaagca
     3661 aggttaagag taatccaata atcaggatgt ttctgagaaa gctaaggtga tgcgatgagt
     3721 aataattttc tgcagaaaca acaacaatat agtttgcctt tccttgagga taggtttgtc
     3781 cggcatagaa ggtgttgccg tttttatagg tcgacttccc cttatcaata atttcctgta
     3841 ggagttcagc aggaagtttt gattcttcag aaattccgcc aacagataat tgaggattta
     3901 ttttgatgat gtattccttt tcctcactca gtttttcaag atgttgctct ctgatctttt
     3961 tataagcgtc tgcagtgagc tcatcctgat ccaaattgta tcgtgcagca attcctgctc
     4021 gtgtttcaag acgtttgtaa aaatcgacat acgaataagc attgagaaaa tagtagattc
     4081 cacctccaaa catcaataga atgatcacgt tcgtaatcac aagaaacaag gctattttcg
     4141 aaggagtttt caagttttaa gaacatatcc catgccaaca actgtgtgga tgagtttgat
     4201 ttctgattcg ctgtcgattt tttttctgag gtaattgata taaacatcaa ctacattggt
     4261 gccaagattg aaatggatgt cccaggccga ttcaagcaac tcagatcgcg acataacttt
     4321 tcctttgttt tttgccaaag tcaataagag ccggtattcc gttgctgtca atgtaattgg
     4381 ttttgcattt cgaaacactt gttttgcgcg atcatctatt gtcagatcgg aaatctcaag
     4441 gatgtgatct gttgtttcag atatcagttc tccggaactt ctccgtagca gagcttttat
     4501 tcgcgcactc agctcaatga atttaaacgg tttgatcaga taatcgtcgg caccggtatt
     4561 cagacctaat gcaacatttt ctgatgttcc caatgcggtt aaaaacaaaa tgggggtttt
     4621 cgtattaaac gagcggatat tttgacaaac ttccaaacca cttttccccg gtaacataat
     4681 gtctagaatg atcaattgaa aaacctgctc ccgtgctagt tt
//
LOCUS       DAOMYG010000257         4686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6369_length_4686_cov_9.973008, whole genome shotgun sequence.
ACCESSION   DAOMYG010000257 DAOMYG010000000
VERSION     DAOMYG010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6369_length_4686_cov_9.973008"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PK610_08960"
     CDS             <1..762
                     /locus_tag="PK610_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HRJ36106.1"
                     /translation="HFSKIKKYAYYYMDDVSVTELPDSATGPCSTPSVKIVSNSERFL
                     FLVDISGSMNANGVMDSLKNALNAAVQKLPPNTEISVVTFSGTPQMVVPFTEAQYARF
                     PGNALDTIKGGGGTNVSHSIEFAYNYLAQEGKRNGTSILLFTDGIFEVPLPSQRRIQQ
                     NYKDHGIRFSSMLIGIRPNFDLQKLATSTSANYSNVTTETIEEQISKEVYKEVPVANK
                     EKTYYTKPYWALIAIRPLKYWLFIGIGYLLIQQYT"
     gene            complement(763..2163)
                     /locus_tag="PK610_08965"
     CDS             complement(763..2163)
                     /locus_tag="PK610_08965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRJ36107.1"
                     /translation="MRFQLLFIACLFSLSGTTQSFTDLQSGFPALNLLTRHAFADVDG
                     DGDQDFAMAGATATSYFGHIYLNDGTGVFSPLAGISFPFLQGSVEWADMDNDGDPDLL
                     VNGMGNSNPLVNFQVYRNDGNLQFTLLNAPFRQLSGYSIWMDYDNDGLKDILSTGYDN
                     SYTNDSTFLYHNDGNGNFSIAIHNLPRMNAEDLSVVDFDNDGDDDFFVMGSSSMAPGS
                     QEISRLYKNDGNGNFTQVPFYFFPLIAGTSDWADYDNDGDADLLYDGMDSLRANNHTL
                     IYRNNGGGNFVQVNANLPLSGEPGSVQWGDVDNDGDLDILIGGPSLLMRNDGNDIFTD
                     ITPGDFPFACPNTLVDIDQDGDLDIFFTVPWGNGATFFRNEGLNGIDNTTRLQLHVFP
                     NPGNGSIRISNLPQEEGILHYQLCDSRGAVLKSGSLVVTNYPLELNWPDLSNGIYTLQ
                     ITGSKTKTSTRIVVMK"
     gene            complement(2266..2811)
                     /locus_tag="PK610_08970"
     CDS             complement(2266..2811)
                     /locus_tag="PK610_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36108.1"
                     /translation="MKKNLLLLIFILQSCFVSAQKPLFIGINPSFTFEKRYGKGEYDV
                     NILPLVFQIPINERVDIRFATIYNYGVRINGNSVQHRGLELALPIFILEEKDASGPSE
                     GFYIAPVVSFSRNRWDGYYHQGLYAEPGYFFKLGQKGGLSIGAQLGATYFRHPNINHP
                     HRTLVRSHFGLKFIIGGWLGA"
     gene            complement(2816..3370)
                     /locus_tag="PK610_08975"
     CDS             complement(2816..3370)
                     /locus_tag="PK610_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36109.1"
                     /translation="MKNVAKFFIEGSILFGVFIISYVIVYMIAGPHFDADGFLRAEFR
                     DTTVEIGMLELSGILAIHLGLGVYLLRALISRFHPPVNGIIYVVLCLSAFFIAQFRAE
                     QFKSIYSLYQLQTITDQTDSISELQNDEQALRAFLSEIEQIPGKSEFVHSMYLYSFII
                     AAMIMTILSLIAYRRKKRTHPSQN"
     gene            complement(3418..>4686)
                     /locus_tag="PK610_08980"
     CDS             complement(3418..>4686)
                     /locus_tag="PK610_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ36110.1"
                     /translation="ISVDNKELIVCACRKDNKRKPDYNNCDLYGTKYERVTNPTTGKL
                     EYKWGELVNLGPNINTPEGWEAQPSLSADGRTLYFAVVRETPGQDPNMDIYYSTRDDK
                     GNWTPAKPLAAINTSGNEKTPFMHSDSKTLYFSAEVGEKYWGAGGYDIFFTRQKADGS
                     WTTPQNIGYPINSTEDEPGMIVSTDGKLAYYSSSKLKGSKGYDIYRFELPEFARPEKV
                     IIVKGQVTDENGDPLANAKIEISYTGDNKKEIVKVDPTDGKYAAVVNVQNGNDAVVTV
                     KAEDRTFDSKLIAANSSTVVKAADLKAEKLEIGKAYTIKDILFASNSFELSAASKFVI
                     DQFIVFLKENPNIKIAIHGHTDDRGVPAENLKLSDNRAKAVMDYIVSKGVAANRLSAQ
                     GFGQNKPKAPNTSEENRRQNRRTEFVIVAK"
BASE COUNT         1292 a         1010 c         1010 g         1374 t
ORIGIN      
        1 catttttcga aaattaaaaa gtacgcctat tattacatgg atgatgtttc ggtaacagaa
       61 ttacccgatt cggctaccgg tccatgcagc acgccttcgg taaaaatcgt ttccaattca
      121 gaacgttttt tatttctggt ggatatttcc ggttccatga atgccaatgg tgtaatggat
      181 tcactgaaga acgcgctcaa tgctgcagtg caaaaattgc caccaaacac cgaaatttca
      241 gttgtcacat tttcgggcac accacaaatg gtggttccgt ttaccgaagc acaatatgca
      301 cgatttcccg gtaatgcact tgataccata aagggtgggg gaggaaccaa tgtttcacat
      361 tccattgaat tcgcttacaa ttatcttgca caggaaggaa aacgtaacgg gacaagtatt
      421 ctgcttttta cagatggaat ttttgaagtc ccacttcctt ctcagcgaag aattcagcag
      481 aattacaaag accatggtat acgttttagc agtatgctta ttggtattcg tccaaatttc
      541 gatttgcaga aattagcaac ttccacatcg gctaattatt cgaatgttac tactgaaacc
      601 attgaagagc aaatctcgaa ggaagtctat aaggaagttc cggttgcaaa caaggaaaaa
      661 acgtattaca ccaaacccta ttgggcgctt attgcaattc gtcctttaaa gtattggttg
      721 ttcataggta tcggatatct gctgatacag cagtacacct gatcatttca ttacaacaat
      781 ccgggtgctt gtttttgttt tcgatccggt aatttgcaaa gtgtaaatac cattggaaag
      841 atccggccaa ttcaactcca gcggataatt cgtaactacg agagatccac tttttagcac
      901 cgcacctcga ctgtcgcata attggtaatg taatattcct tcctcctgtg gcagattgct
      961 gatacggata gatccatttc cgggattggg aaaaacatgc agttgcagtc gtgtggtatt
     1021 atcaattccg tttaatcctt cgttccggaa aaaggttgcg ccatttcccc agggtacagt
     1081 gaagaaaata tcgagatcgc catcctgatc gatgtctacc aatgtgttcg gacaagcaaa
     1141 ggggaaatca ccgggtgtga tatcagtaaa aatatcgtta ccatcatttc gcatcagcaa
     1201 acttggacct ccaattaaaa tgtccaaatc gccatcgtta tccacatcgc cccattgaac
     1261 ggagccgggt tctccggaaa gcggaaggtt tgcattgacc tgcacaaaat ttcctccgcc
     1321 gttgttgcgg taaatcaatg tatgattgtt ggcgcgaaga gaatccattc cgtcgtataa
     1381 cagatccgca tcaccatcat tatcatagtc ggcccaatcg gaagttcctg ctatgagagg
     1441 aaagaaataa aaaggaacct gcgtaaagtt tccgttcccg tcgttcttat aaagacgcga
     1501 aatttcctgc gagccgggtg ccatagaaga tgaacccatc acaaaaaaat cgtcatctcc
     1561 gtcattatca aaatccacca ccgaaagatc ttctgcattc attcgtggca aattgtggat
     1621 ggcaatgctg aagtttccgt ttccgtcgtt atgataaaga aaagtggaat cattggtata
     1681 gctgttatcg tagcctgtac tcaggatgtc ttttaaacca tcattgtcat aatccatcca
     1741 gatgctatat cccgaaagtt gacgaaaagg tgcgtttagt aaggtgaatt gcaggttgcc
     1801 atcgtttctg tacacctgaa aatttaccag cggattcgaa tttcccattc cattcaccaa
     1861 gagatccgga tcgccgtcgt tatccatatc ggcccattct acagatccct gaaggaaagg
     1921 gaaagaaatt cctgctaagg gactaaaaac accagtgcca tcattgaggt agatgtgtcc
     1981 gaaataactc gttgctgttg cacctgccat agcaaaatcc tgatcgccgt cgccatccac
     2041 atcggcgaat gcatgtctgg tgagaagatt caaagcagga aaaccggatt gaagatcggt
     2101 aaacgattgc gttgttcccg aaagagaaaa caaacaagca atgaaaagga gttgaaaacg
     2161 cataaatgag ttatagtaaa cagacgatgc aataacgcat attgatgcac ggtgcagcaa
     2221 ccaaaatctg ttattattca accgatgcta caacaggcaa tggcattatg ctcccaacca
     2281 tccgccgatt atgaatttta acccgaaatg actccttacc agggtgcggt gcggatgatt
     2341 gatattgggg tgtctgaaat aggttgcgcc taattgagct ccgatcgata agccgccttt
     2401 ttgaccgagc ttaaaaaaat atcccggttc agcgtacaat ccctggtggt aatacccatc
     2461 ccaacggttg cgggaaaaac ttacaaccgg agctatatag aatccctcgg aaggtccgct
     2521 tgcatctttc tcttcgagaa tgaaaatagg taaggcgagt tccagtccgc ggtgttgcac
     2581 gctgtttccg ttgatgcgta ctccgtaatt gtaaatggtt gcaaaacgga tgtcgacgcg
     2641 ttcgttgatg ggaatctgaa ataccagcgg aagaatattg acatcgtatt cgccttttcc
     2701 atatcgtttt tcgaaggtaa aggagggatt gatgccaata aacaatggct tttgtgcgct
     2761 gacaaagcag gattgcaaaa tgaaaattaa aagcaacaaa ttctttttca tgcaactagt
     2821 tttgagaggg gtgggtcctt ttttttctgc ggtacgcgat caaagaaaga atggtcatga
     2881 tcattgctgc gataataaac gaatacaaat acatggaatg cacaaattca gatttacctg
     2941 gaatttgttc aatttcactc aggaaagcac gcaaagcctg ttcgtcgttt tgcagttcac
     3001 tgatgctgtc ggtttgatcg gttatcgttt gaagctgata aagtgaataa atggatttga
     3061 actgttctgc tctgaattgc gcaatgaaaa atgcacttaa acaaagtacc acataaataa
     3121 ttccgtttac cggaggatga aatcgcgaaa tcagggcacg tagtaaatac acccccaatc
     3181 cgaggtgaat ggccagaatt cctgagagtt caagcatccc aatttctacg gtggtgtcgc
     3241 ggaattcagc tcttaaaaaa ccatctgcat cgaaatgcgg accggcaatc atgtagacaa
     3301 taacatagct gatgatgaac acgccaaaaa ggatgcttcc ttcaataaaa aatttggcga
     3361 cgtttttcat tcctgtttca ggttaatatt tgcgttcagg tttcttgcag gaaaacttta
     3421 cttcgcaaca attacaaatt ccgttctacg gttctgcctt ctgttttctt ctgatgtatt
     3481 gggtgctttt ggtttgttct gaccaaatcc ctgtgcgctc aatctgtttg cagctactcc
     3541 tttcgaaaca atgtagtcca tcacagcttt cgcacgatta tcggatagtt tcaggttctc
     3601 tgcaggtaca ccacggtcat cggtatgacc atgaatcgca attttaatat tcgggttttc
     3661 tttcagaaag actataaact gatcgatcac aaatttgctt gcagccgaaa gttcgaacga
     3721 gttggatgca aacaagatgt cttttatcgt gtaagcttta ccgatttcca gtttttctgc
     3781 tttcagatcg gctgctttta caacagtcga tgaattggca gcaataagtt tactgtcgaa
     3841 cgtgcggtct tctgctttaa cggttacaac agcgtcgttt ccgttctgca cgtttaccac
     3901 agctgcatat ttcccatcgg taggatcaac ttttacaatt tcttttttgt tatcgccggt
     3961 gtacgagatt tcaattttcg cattggcaag cggatctccg ttttcgtcgg ttacctgtcc
     4021 tttaacaatg attacttttt cagggcgcgc aaattcgggt aattcaaatc ggtagatatc
     4081 gtatcctttt gatcctttta gtttcgagga tgagtagtaa gccagttttc cgtcagtgct
     4141 gacaatcatt ccgggttcgt cttccgtgct gttgattggg tagccgatgt tttgtggtgt
     4201 ggtccagctt ccatctgctt tttgtcgtgt gaaaaatata tcatatcctc cggcacccca
     4261 atatttttct cctacttcgg cagagaaata aagcgttttt gaatcgctgt gcatgaaagg
     4321 tgttttctca ttgccggatg tgttgatcgc agcaagcggt tttgccggtg tccagtttcc
     4381 tttgtcgtcg cgggtggaat aataaatatc catattcgga tcttgccccg gagtttcgcg
     4441 cactactgca aaataaagag tgcgtccgtc ggccgataag gaaggttgtg cttcccatcc
     4501 ttcgggtgta ttgatgttcg gacccagatt caccaattct ccccacttgt attcgagctt
     4561 tccggttgtg ggatttgtaa cgcgttcgta tttggtgcca tacaaatcgc aattgttgta
     4621 atcgggtttg cgtttgttgt ccttacggca ggcacaaaca atgagttcct tgttatctac
     4681 cgaaat
//
LOCUS       DAOMYG010000258         4685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6374_length_4685_cov_10.063067, whole genome shotgun sequence.
ACCESSION   DAOMYG010000258 DAOMYG010000000
VERSION     DAOMYG010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6374_length_4685_cov_10.063067"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..2891)
                     /locus_tag="PK610_08985"
     CDS             complement(54..2891)
                     /locus_tag="PK610_08985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ36111.1"
                     /translation="MKKSLLLFIGVAGMLCFKPVKAQLNSESTVIYCDEFHVTRPLRD
                     LAKEGMEDIKRIEEARRKKFESPDRKHRTAAIPEHTLEDGAEYATDEKFIQKDNGSRD
                     NRAPLVNWAGLTASGFRPFDPSGAAGPNHYIQAINATTYRVYNKANGGNMATGLIGSL
                     WSPSTPNDGDPIIMYDRFADRWFISQFGTSGNRMYIAISTTADPLGSYYTYTFTSPQF
                     PDYLKFSIWSNGYYMTANTSTQRVFAFERDAMLAGSATARAVSATFNPPRPGGFFCPL
                     PGDADGNGGLPAASAPCPIFSFSDNGWGGSNIDAIQIYHATVNWVPATPTLTITNAGP
                     VATAAFDGSYNSGWNDISQPGTSQKLDGIGGVLNYRAQWRKWSGYNSVVLTWGVRISA
                     SQRSIMWCELRQNQGTGVWSMYQQGVFTPDAASRWNGSISMDDNGNIALAYAKSDAST
                     IKPSLCYTGRLSTDPLGQMTFAEETAIAGLAVQTSGNRYGDYSHTSLDPDGVTFWHTG
                     EWMGGTNGATTAARTRIYSFQLQASTQAVVAITSNDADNAVCIGTNVTFTAAPTNGGT
                     TPTYQWLVNGNPVGTNSPTFSSSTLSTGDVVTCVMTSNMPGVTGNPATSNSVTMTVSP
                     VPTTPSPVANTPVCTNGTLNLTTGAVTGATYAWTGPSGYVSNTQNPNRPNVTSGMAGT
                     YSLTVTVGGCSSAAGSVTVTVNPSPSAPGASTNSPVCSGNTIQLNASTVANATYAWTG
                     PNGFTSTTQNPTITGSTTANTGSYSVVAIGTNGCSSTASSASVIVNATPATPTITQNG
                     GILTSSSSSGNQWYLNGSPISGATGQNYTPTTNGIYTVVVTLSGCSSTASSQLNVTNV
                     GIDEENLNMLMVFPNPTNGKFSITFESVSGKEYVLEIFNDIGQVVFSETFRNGGSIVR
                     PIDLGENASGIYTVTLKSEGKSTIKKVVVQR"
     gene            complement(3036..4019)
                     /locus_tag="PK610_08990"
     CDS             complement(3036..4019)
                     /locus_tag="PK610_08990"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRJ36112.1"
                     /translation="MAKAAITAVGGYVPEYVLTNEEIETMMDTTSEWIVTRSGITERR
                     IMKDKTKASAFMAAEAAKEVLKKSGTSAKDIELIIVATVTPDHQYPATANIVADMIGA
                     TNAWSFDVQAACSSFIYALQTASKFIESGAHKKVIVIGSDKMSSIMDYTDRSTSILFG
                     DGAGAVLLEPSADETGILDARMYVDGSGKKYLYQEAGGSLQPASFETVLNKQHFVTME
                     GQSVFKVAVVKMADVAEEIMVRNKLGANDIAFLVPHQANKRIIEATADRMGVGMEKVM
                     LNIQRYGNTTSATLPLCLWEWEKQLKKGDNLILTTFGAGFTWGGMLVRWSY"
     gene            4168..>4685
                     /locus_tag="PK610_08995"
     CDS             4168..>4685
                     /locus_tag="PK610_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36113.1"
                     /translation="MSKLKSEDLFRLIKSLSPSEKRAFTLQSGKSSGTKYMRLFEEMV
                     KLEEYNESKLLKKIPELPPHQISNLKAQLYKHLLKTIQHLESREVEEVQLTSMIDHAR
                     ILYNRCLYKECLTHIDKAKRRAKNTDSFLLLLELLELEKLAVLRSPGEYSEDRVNRII
                     SETEKTANAIRN"
BASE COUNT         1429 a          994 c          959 g         1303 t
ORIGIN      
        1 aaaaaaaacc ccccgccata agcggggttt tttatttaat aatagttgta agattatctc
       61 tgaacaacaa ctttcttaat agtgctttta ccttcacttt taagtgttac agtatatata
      121 cctgaagcat tttctccaag atcaattgga cgaacaattg agccaccatt acggaaagtc
      181 tcgctgaata caacctgacc tatatcgttg aagatttcaa gtacgtattc cttacctgaa
      241 acagattcga aagtgatcga gaatttacca ttcgttggat tagggaatac catcaacata
      301 ttgaggttct cttcatcaat tccaacattg gtaacattaa gttgcgaaga tgcagtagaa
      361 gaacatccgc tcaatgttac aacaacagtg tatataccat ttgttgtagg ggtataattc
      421 tgtcctgttg cacctgaaat cgggctacca tttaggtacc actgattacc tgatgatgaa
      481 cttgaggtta gtattccacc attctgcgta attgttggtg tggccggtgt agcatttaca
      541 attacagatg cagaagaagc agtacttgaa catccgtttg ttccgattgc aacaacagaa
      601 tatgaacctg tattagcagt tgtggaacct gtgatcgttg ggttttgcgt ggttgaagta
      661 aatccatttg gaccagtcca tgcataagta gcgtttgcaa cagtacttgc attcaactga
      721 atggtattac ctgaacacac cggagaattc gtagatgcac ccggagcaga aggtgaagga
      781 ttaacagtta ccgttacaga acctgcagca cttgaacaac caccaaccgt tacagtaagt
      841 gaatatgttc cggccatacc tgaagttaca ttaggtcgat tcgggttttg tgtattggaa
      901 acataaccac ttggacctgt ccatgcataa gttgcaccgg taacagcacc agtagttaaa
      961 ttgagtgtac cgtttgtaca aacaggtgtg tttgccacag gtgaaggcgt agtaggaaca
     1021 ggactaacag tcatggttac gctattcgaa gttgcaggat ttcctgttac accgggcata
     1081 ttcgaagtca ttacgcaggt aacaacatca ccagttgaaa gagtagatga tgaaaaagtt
     1141 gggctatttg ttccaaccgg atttccatta actaaccact gataggttgg tgttgttcct
     1201 ccgttggtag gagcagcagt aaatgttacg ttggttccaa tacaaaccgc attatccgca
     1261 tcatttgaag taatagctac aacagcctga gtacttgcct gaagctggaa agaataaata
     1321 cgtgtacgag cagccgttgt tgcaccattt gtaccgccca tccattctcc agtatgccag
     1381 aaagtaacgc catcaggatc aagagatgta tgcgaataat caccataacg gtttccactg
     1441 gtttgaactg ctaaaccagc aattgcagtt tcttctgcaa aggtcatttg tccaagcgga
     1501 tctgtagaaa ggcgtcctgt gtagcaaagt gatggtttaa ttgttgaagc atcagatttt
     1561 gcatatgcaa gcgcaatatt tccattatca tccatagaga tagaaccatt ccaacggctg
     1621 gctgcatcag gagtaaatac cccttgctgg tacattgacc aaacaccggt accctggttt
     1681 tggcgaagtt cgcaccacat aattgagcgt tgtgatgcac taatacgtac accccaggtc
     1741 aatacaaccg agttgtaacc cgaccatttt ctccattgtg cgcgatagtt taatacgcca
     1801 ccaataccat ctaatttttg agatgttcca ggctgtgaaa tatcattcca gcctgaattg
     1861 tatgaaccat cgaatgcagc agttgctaca ggaccggcat tagtaattgt aagtgtagga
     1921 gttgcaggaa cccagttaac tgttgcatgg tatatttgaa tagcatcaat gtttgaccca
     1981 ccccaaccat tatctgagaa ggagaaaatt gggcatggtg cacttgcagc gggtaatcca
     2041 ccatttccat cggcgtcacc cggtagcggg cagaaaaatc cgcccggacg tggaggattg
     2101 aacgttgcag atactgcacg tgcagttgca ctaccagcaa gcattgcatc acgttcaaat
     2161 gcaaatacgc gttgagttga tgtatttgcg gtcatataat acccattact ccagatcgaa
     2221 aatttcaggt aatccggaaa ctgaggtgaa gtaaacgtat aagtgtaata cgatcccaat
     2281 gggtctgccg ttgtggaaat cgcgatatac attctgttac ctgacgtacc aaactgtgaa
     2341 ataaaccagc ggtctgcaaa acgatcgtac ataatgatag ggtcaccatc atttggtgtg
     2401 gagggtgacc acagacttcc gataagtcct gttgccatat ttcctccatt ggctttatta
     2461 tacaccctgt acgtagtagc attaatagcc tggatgtaat ggttaggtcc agcagcaccg
     2521 gatgggtcaa aaggacgaaa tcctgaagcg gtaaggcctg cccaattcac cagaggtgcg
     2581 cggttatcgc gagatccatt gtctttttga atgaattttt catcggtagc atattctgca
     2641 ccatcttcaa gagtatgctc aggaattgct gcagtacggt gttttctgtc gggagattcg
     2701 aacttcttac ggcgcgcctc ttcgatacgt ttaatatctt ccatcccttc cttagcaaga
     2761 tcgcgaagtg ggcgggtgac atggaactca tcgcagtaga taactgtaga ttccgaattt
     2821 aattgggcct taaccggttt aaaacaaagc attccggcaa ctccaatgaa aagaagtaga
     2881 gattttttca tgacaagcat agatagtttg aggcccttaa tatactaaag ggtggtcaag
     2941 gttccgaata aaaaatgacc aaaaattgac ttcctatgcc taattaatat gattgatagg
     3001 caattgcaat gaagtgaaaa aatcagatag ccacattaat aggaccagcg aaccaacatg
     3061 cctccccagg taaatcctgc gccgaaagtg gtgagaatca ggttatctcc tttttttaat
     3121 tgcttttccc attcccaaag acataaaggc agggtagctg atgttgtatt accgtaacgc
     3181 tgaatgttta acatcacttt ttccatacca acacccatcc tgtcggcagt tgcttcaata
     3241 attcgtttat tagcctgatg tggaactaaa aatgcaatat cattagcacc gagtttattg
     3301 cgaaccatta tttcctctgc aacatcggcc attttcacaa ctgctacctt gaaaacagat
     3361 tgcccttcca tggtaacgaa atgctgttta ttcaaaacag tttcgaagga cgcaggctga
     3421 agtgaaccgc ctgcttcctg atacaggtat tttttcccac taccatcaac atacatcctt
     3481 gcatccagaa ttccggtttc atctgcagac ggctcaagca aaactgcacc ggctccatca
     3541 ccaaaaagaa tagaagttga acgatcggtg taatccataa tactagacat cttatctgaa
     3601 ccaattacaa tcaccttttt atgagcaccg gattcaatga atttacttgc tgtctgtaac
     3661 gcataaataa aactggaaca agccgcctga acgtcaaaac tccatgcgtt ggttgcacct
     3721 atcatatcag caacaatatt agcagtggca ggatattggt gatcgggtgt tactgttgcc
     3781 acaataataa gctcaatatc ttttgcactt gtccctgact tcttaagaac ttccttagca
     3841 gcttcagcag ccataaatgc ggatgccttg gttttatcct tcattatacg gcgttcagta
     3901 ataccactgc gtgttacaat ccactcactg gtggtatcca tcatcgtttc aatctcctcg
     3961 ttagtaagta catactccgg aacatatccg ccaacagcag taattgcagc ttttgccatg
     4021 tgatttgatt ttggacaaaa ataaactgaa taagtccaaa gaaaatttaa gaaagatata
     4081 tattacgatg tctaaattga attactaaac aacaaattca actattttac tattaaaaat
     4141 acttagattt ggcaaaatca ttctacgatg tccaaattga aatcggagga tcttttcaga
     4201 ctaataaagt cattaagtcc ttccgaaaag cgcgcattta cattgcaatc cggaaaatcg
     4261 tcaggaacca aatacatgcg cttatttgag gaaatggtga agttagaaga atacaatgag
     4321 agcaagctgc taaaaaaaat tccggagctt ccgcctcatc aaatttccaa cctcaaggcg
     4381 caattgtata aacatctgct caaaacgata caacatcttg aaagcagaga agtagaagaa
     4441 gtgcagttaa cctctatgat agatcatgca cggattttgt ataaccgttg tttgtataag
     4501 gaatgcttaa cccacatcga caaggcaaaa cgaagagcaa aaaatacaga tagtttctta
     4561 ttgttactgg aactgctcga gctagaaaaa cttgctgtat tgcgttcgcc gggagagtat
     4621 tcggaggata gggtaaatag gataatttca gaaacagaaa aaactgcaaa cgccattcgc
     4681 aatat
//
LOCUS       DAOMYG010000259         4674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6387_length_4674_cov_8.100022, whole genome shotgun sequence.
ACCESSION   DAOMYG010000259 DAOMYG010000000
VERSION     DAOMYG010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6387_length_4674_cov_8.100022"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="PK610_09000"
     CDS             <1..1288
                     /locus_tag="PK610_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36114.1"
                     /translation="YDPNALYLLGRAYHFNGEFDKAIKAFTRFIELGSGAPDNRKEAG
                     HYIDYCQNAKELIKFPVKVSFENLGRNVNSSNPDYLPFLPVDESFILFTSRRNDGSYE
                     RTNGGYFSNIYISTKQDGKYGKAVMLEGVNQFGTEENIVGLSNSGNVAIIFRENPNGS
                     SELRMVFRKDGKFGQELPLDEKINSPRYTEIAATLSSDSSAIYFASDRPGGYGGIDLY
                     ACRKLPNGKWSDPQNLGPSVNTLYDEDFPSVSPDGNTLYFSSKGHTSMGGYDLFKATF
                     NEEKQRFMDVRSLGYPINTLGDDMNFRVSEDGKYGYLAAIRPEGFGDMDIYKVNFLEV
                     ETRYTVFKGVIRSADSTLKPNATFISVTNLATGELFGEYQPNSSTMKYVVILPPGRYS
                     FFVESEGFSGFQEEIEISDDGQNRFEIIHDILLQRE"
     gene            1285..1917
                     /locus_tag="PK610_09005"
     CDS             1285..1917
                     /locus_tag="PK610_09005"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRJ36115.1"
                     /translation="MMQYDITTLLVAANVIVSLMCFQNKDLFYKLSLNPYSIKHDRQW
                     YRLITHAFVHADFFHLFINMFVLYNFGSILEILFKAHFGDKATMLFILLYTGGIVFAT
                     PTALKRHQDNPGYNAVGASGVTSAIVFSFIALLPGHYLYLFAALPIPAFVFGILYLVF
                     EAVMDKRGRGNIAHDAHFWGAVFGFVFTLLMDVELIPEFFRQIIQFFESL"
     gene            1914..2396
                     /locus_tag="PK610_09010"
     CDS             1914..2396
                     /locus_tag="PK610_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HRJ36116.1"
                     /translation="MKVKALFLDRDGVINMERGDYTWKKEDISLLPDLIPFLNSAKKK
                     GYTFFVITNQGGIGKGIYTTKEYLEVEQYIADLLAGENLFFEEVYYCPHHPTTGKCLC
                     RKPGSLLLEKAIARFNIDAGISVMIGDRERDVVAANGAGVKGILIESNSPLLPILEQI
                     "
     gene            2400..3266
                     /locus_tag="PK610_09015"
     CDS             2400..3266
                     /locus_tag="PK610_09015"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRJ36117.1"
                     /translation="MTPFRFAMCDGQLVEASQPYLTAENRSFRYGDGLFETMRVMQGR
                     PAFLESHIARLLEGMAMLKMEIPDYFSLSFFAEKINELLKANGITDGARLRLAVYRNS
                     GGFYLPQDNGAGFVIEALPIIHHHYQLNEDGLTVDLYTEIRKPVNKLSIYKTANSLLY
                     IMSALWAKQNKLDDALIVNDKGNIIEASSSNLFIICNGVLYTPALSDGCLAGVMRMQI
                     INLALEHKIKVYECSLNPQNLLVADEIFLTSAGKGLQWVSSYKMKRYFNSLTRTLVDR
                     LNEKVRSGVMEG"
     gene            3284..4603
                     /locus_tag="PK610_09020"
                     /pseudo
     CDS             3284..4603
                     /locus_tag="PK610_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
BASE COUNT         1332 a          998 c         1059 g         1285 t
ORIGIN      
        1 gtacgatccc aatgcgctgt atttgttagg acgtgcctac cattttaatg gggaattcga
       61 taaagctatt aaagcattta cccgatttat tgaactgggc agcggtgctc cggataaccg
      121 caaagaggcg ggacattaca tcgattattg tcagaatgca aaagaattga tcaaattccc
      181 tgtaaaggtt tccttcgaaa atctgggtag aaatgtcaat tcctccaatc ccgattatct
      241 gccattcctt cccgtcgatg aaagttttat tctctttact tcccgaagaa acgatggttc
      301 gtatgaacgt accaatggtg gttatttctc aaatatttac atcagtacca aacaagacgg
      361 caaatatgga aaagcagtaa tgctcgaagg ggttaaccag ttcggaacag aagaaaatat
      421 tgtgggactc tccaattcgg gaaatgtggc tattattttc agggagaacc cgaacggaag
      481 ttcagagtta cggatggttt tcaggaaaga tggaaaattc ggacaggaat taccgctcga
      541 tgaaaagatc aattctcctc gttataccga aattgcggca acactgagct ccgatagcag
      601 tgccatctat tttgccagcg atcgtccagg tggatacggt ggtatcgatc tgtacgcttg
      661 cagaaaactt cccaatggaa aatggtcgga tccacaaaat cttggtccct ccgtaaatac
      721 cttgtacgat gaggatttcc cctctgtttc tccggatggc aatacacttt atttctcctc
      781 caagggacat acttcaatgg gaggatacga tctgttcaag gccacattca acgaggaaaa
      841 acaacgtttt atggatgtcc gtagtctggg ttatcccatc aacacccttg gagatgatat
      901 gaatttcagg gtgagtgaag acggaaaata cgggtacctg gcagccattc gaccggaagg
      961 atttggagat atggatatct acaaagtcaa ttttctggaa gtcgaaacca gatacacagt
     1021 tttcaaaggt gttattcgtt ctgccgattc taccctcaaa cccaacgcaa cctttatcag
     1081 tgtaaccaac ctggccacgg gtgaattgtt tggagaatac caacctaaca gcagcaccat
     1141 gaaatacgtg gttattctgc ctcccggtcg gtacagcttt tttgtggaat cagaaggatt
     1201 cagcggattt caggaagaga tcgaaataag cgatgatggt cagaaccgtt ttgagatcat
     1261 tcatgatata ttgcttcagc gcgaatgatg caatatgata tcacaacttt acttgtcgca
     1321 gccaatgtga tagtttcatt gatgtgcttt cagaacaagg atctgtttta taagctgtca
     1381 ctcaatccct acagcataaa gcacgaccga cagtggtaca gattgatcac gcatgctttt
     1441 gttcatgccg attttttcca tttgttcatc aatatgtttg tgctttacaa tttcgggagt
     1501 attctcgaaa tcctcttcaa agcacatttt ggcgacaaag caaccatgct ctttatcctg
     1561 ctctataccg gaggaattgt tttcgccacg ccaacagcat taaagcggca ccaggataac
     1621 cccggttata acgccgttgg tgcttcgggg gtcacatctg ccattgtgtt ttccttcatt
     1681 gcattgttgc caggacatta cctctatttg tttgcagcat tgccaattcc cgcatttgta
     1741 tttggaatac tgtatcttgt ttttgaggca gtaatggata aaagaggtcg gggaaatatt
     1801 gcccatgatg cccatttctg gggagcagta ttcggatttg tctttacttt gctgatggat
     1861 gtagaactga taccggaatt tttccgacag atcattcagt ttttcgaatc actatgaagg
     1921 taaaagcatt gtttttggac cgcgatgggg tgatcaatat ggaacgcggt gattatacct
     1981 ggaaaaagga agatatttcc ctgttaccgg atctaattcc ctttcttaac agtgccaaga
     2041 aaaagggata taccttcttt gtaatcacca atcagggagg tatcggaaaa gggatatata
     2101 ccaccaaaga ataccttgaa gttgagcaat acattgccga tttgcttgcc ggggaaaatc
     2161 tcttttttga ggaagtttat tattgtccgc atcaccccac aacgggtaaa tgcctttgcc
     2221 gaaagccggg ttctttactg ctcgaaaagg cgatagcccg gtttaatata gatgcgggca
     2281 tatcggttat gatcggcgat cgtgaaagag atgtagtggc cgctaacggt gccggggtta
     2341 aggggatttt aattgagagt aattctccgc ttttgcctat attagagcaa atttgaggca
     2401 tgacaccatt ccgttttgca atgtgcgacg gtcagctggt tgaggcatcg cagccttatc
     2461 ttactgctga aaaccggtcc ttcaggtacg gtgatggtct ttttgagacc atgcgcgtta
     2521 tgcagggacg acccgcattt cttgagagtc atattgcacg tttattggag ggtatggcga
     2581 tgctgaaaat ggagattccc gactattttt ccctttcatt tttcgctgaa aaaatcaatg
     2641 aattactaaa ggccaacggc attaccgatg gtgcccggtt gcgactggcg gtgtatcgca
     2701 acagtggcgg attttacctt ccgcaggata acggtgcagg atttgtaatt gaagcattgc
     2761 ccattattca tcatcattac caattgaacg aggatggatt aaccgtagat ctttatacag
     2821 agatccggaa gccggttaat aaactttcca tctacaaaac agccaattcc ctcttgtaca
     2881 tcatgtctgc actttgggcc aagcagaata aactcgatga tgcactcatt gtaaatgaca
     2941 agggaaacat catcgaagcc agcagcagta atttgttcat catttgcaac ggggtattat
     3001 atactcccgc actttccgat ggatgccttg caggtgtgat gcgcatgcag atcattaatc
     3061 ttgccctcga acacaaaata aaagtgtacg aatgttcact caatccgcaa aaccttcttg
     3121 ttgccgacga gatcttcctc accagcgcag gcaaaggatt gcaatgggtg agcagctaca
     3181 aaatgaaacg ctattttaat tcactcaccc gaacactcgt cgaccggctt aatgaaaaag
     3241 ttaggtccgg cgtaatggaa ggataaatta ttcaactatg ctgatcgata ttccaaattc
     3301 aggcaaagcc cgtgtggtag taataggagg aggtttcgga ggcatacgcc tggcaaaagc
     3361 actttcaaaa aaaaatgttc aggttgtact gatcgataaa aacaattacc acgcttttca
     3421 accgctcttg tatcaggtag ccactgcggg actcgaagcg gattccattg cctatcctat
     3481 ccgcaagatc tttggtaaat cgaagaatgt ttttttcaga atgggcgaag tacaacgcat
     3541 cattcccgag gaaaagaaaa tagagatcga caacgaccac ctgttttacg attatctggt
     3601 tatcgcaacc ggtgctaaaa ccaattattt cggaaacgaa ggactcacca tttcttctat
     3661 gccaatgaaa agtgttcctg aagcactcga tttgcgcagt atgatccttc agaatttcga
     3721 aaagatccta accatcacca acgaacgcaa aaaacagagt tatatgaact ttgtaattgc
     3781 aggagccggt ccaactggtg tggagttagc cggtgcattg ggagaattaa agaaacacgt
     3841 tttcccccgc gactatcccg aactcgattt ggggaagatg aatatttacc tcgttcaatc
     3901 cggcgatcgt gttttaccca tgctctccga aaaaagctca ctcaaagcca aacgctacct
     3961 cgaggaactg ggagtgcagg tggtactcaa tacccgcgtg ctcgattaca atggcgacta
     4021 cgtgcaaacc aataccggaa acgatcttat tgcacgtacg ctcatctgga cagcaggtgt
     4081 aacgggcaat cccatcgacg gactcgatcc caaactcatt accaaaaccg ggagatatct
     4141 tgtagatgaa tacaatcgcg tgcaaggtta ttcagatgta tttgccattg gtgatgtagc
     4201 ctgtatgatc acaaaggaat tcgaaaacgg acatccacag gttgctcccg ccgcaataca
     4261 gcaagccacg cattttgcga agaatatttt gctgcagatc aataaaaaaa ccatgaagcc
     4321 gttcaagtac aaagacaaag gttccatggc caccattggt taaaaccgtg cagtggtgga
     4381 ggtaggcaat tttaaaattg gaggagccat tgcatggatg atgtggatgt ttgtgcacct
     4441 gatgtcgcta gttggtttcc gcaatcgctt catgactttt atcaactgga ctaaaaacta
     4501 cttcagctcc gaccgcggaa tgcgattgat cattcgtgaa ttcgatctta cagaagtaaa
     4561 acgccgccgc aaacgcgaaa tggaagcgga gaagagtaag tgagggtttt acagatggat
     4621 ggatggatgg atggaagatt actgttgtga tttgctttca ttctttgatc tact
//
LOCUS       DAOMYG010000260         4661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6423_length_4661_cov_13.631350, whole genome shotgun sequence.
ACCESSION   DAOMYG010000260 DAOMYG010000000
VERSION     DAOMYG010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6423_length_4661_cov_13.631350"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4191
                     /locus_tag="PK610_09025"
     CDS             <1..4191
                     /locus_tag="PK610_09025"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ36118.1"
                     /translation="GDFNTEVKFAHDGHAENFFFTPSGVVIMLEWQQKRIKSDAEKAA
                     RLERKKKGFASREEFMAFEKEGKKIDYKSDALYCQWEGGNPNPEIVAERKNSFTHSYS
                     FYDKSRQLQNVSNLNSYLKLTYKNVYPKIDIVYEFHPEGGLKYSVVVHPGGNIADVKL
                     KYSKDFLLHPDGTIHTPTLFGNIIDHAPLTFYADNYRKVVESSYKINGRTIGFNVGNY
                     DQSKTLVIDPWTQSPTFNTNWDCIWEVETDASGNTYIIGGVMPLQLIKYNNTGVQQFV
                     YNTPYDTTAWLGTFATDDAGNSYITNGSAAAIVKVNTTGTVVWNNANPGGILTLTEFW
                     NIAFNCDQTMLVVGGTGSVGFLPTQPLPWVYNINMNTGNVINSLQVAGGGAILNSQEV
                     RSITATKDDKYYFLTHDTIGYISSDLSLCPGQNGPFKIENGGYNLSYKCENFRYDNTG
                     LEALAYYGNFVFVNRGTRLDKRDLATGAIVATTPIPGGGFTASLGQNQAHNSGIAIDN
                     CGNIFVGSKTGLYKFDQSLGQTGFFATTFNVYDVDITSTGEVVVAGSTGDANSNSRTG
                     SVQSVAATACAQQSTTCCDPTVCNVPSLCTSNSPITLSAGSPGGTWSISPATPALNTS
                     TGVFTPASAAPGTYTVTYTLACGNDTQTITVANCSALTVCQESNGTYTVSGGAGGPFT
                     WQSGAMVTPCVSGFSFCNATPFSANGTPVLTWTTFTTGTNVTIGGGITQIQVLDGFGN
                     SVIYNPVSSIPACAACPTITVTTSNVQNVACFGQSTGSFTVAASGGAGPYTYTILNGA
                     TTVASFPNVAGSQNVTGLPAGTYTINVLDAASCPGTVNVTITQPATAVSVSATSTPAT
                     CGSNNGTVTGSGTGGTGAITYSWNTTPIQNTATATGLGAGTYTVTATDANGCQATATA
                     TITSSGGPTVTTTQVNVSCFGGNNATATASATGGTGTITYVWSTTPAQTAATATGLTS
                     GTYTVTATDGGGCSGSASVTITQPATAVSVTANATAATCGLSDGTATANGTGGTGAIS
                     YSWNTSPVQTTATATGLAAGPYIVTATDANGCTATATATVNNTGAPTITISSQNNPAC
                     NNGTNGTATVSASGGTGTLNYSWNTTPVQTGATANNLGAGTYTATVTDGSGCSASVVV
                     TITNPPAITASVSVTNANCAASDGSATVNASGGTGTLSYSWNTTPVQTTASITALAPG
                     AYSVTVTDANGCSTTTGGTVATVGTANIDAGNDVLIPLGASTVLNATGGTVYTWTPAT
                     GLSCTNCASPTASPTTTTTYIVSGTDANGCFGSDTVVVFVELPCGEIFVPNAFSPNGD
                     NENDVLRIYGNCINELYFVIFDRWGEKVFITTNPAEVWDGTIRDKAMNNAVFFYYLSA
                     TLNNGDVVEKSGNITLIK"
     gene            4200..>4661
                     /locus_tag="PK610_09030"
     CDS             4200..>4661
                     /locus_tag="PK610_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRJ36119.1"
                     /translation="MKNKILSLTLGLVAGVVSAQDIHFSQFYMAPIQLNPGAAGTEAD
                     IRVHLNYKEQWKSVASPYKTIGGSYDMRFKKTKSGYFGGGIFFYSDRAGDARMGVAQG
                     NLNLAYHLVIGQGQTLGLGVMGGYAQRSVNTTNMQWGNQYDGMAYNSSLPAG"
BASE COUNT         1425 a         1056 c         1006 g         1174 t
ORIGIN      
        1 ggagatttca acaccgaagt taagttcgcg catgatggac atgccgaaaa tttctttttt
       61 accccatcag gtgttgtaat aatgctagaa tggcagcaaa agcgcattaa aagcgatgct
      121 gaaaaagcag cacgactgga gcgcaagaaa aaaggctttg ccagcagaga agagtttatg
      181 gctttcgaga aagaaggaaa aaaaatcgat tataaatcgg acgcgttgta ttgccaatgg
      241 gaaggcggaa atccgaatcc tgaaatcgtt gctgaaagga aaaattcatt tacacattcc
      301 tacagcttct acgataaaag caggcagctg caaaatgtca gcaacctcaa ttcctattta
      361 aagctgacct acaaaaacgt gtatccaaaa atcgatatcg tttatgaatt tcatccggaa
      421 ggtggattaa aatattctgt cgttgtgcat cctggcggta atatcgctga tgttaaatta
      481 aagtattcaa aagatttcct gcttcatcct gacggaacta ttcatacccc tactttattt
      541 gggaacatca tcgatcatgc accactaaca ttttatgccg ataattaccg taaagtagtg
      601 gaatcatctt ataaaatcaa tggtcgcaca attggattta atgttggaaa ttacgatcag
      661 agcaaaaccc tggtaatcga cccatggacc caatctccaa cattcaacac caactgggat
      721 tgtatttggg aagttgaaac agatgcttca ggaaacacct acattattgg tggtgtaatg
      781 ccattgcagc taattaagta caacaataca ggagtgcaac aatttgttta caacactcct
      841 tacgatacaa ctgcatggct tggaacattt gcaaccgatg atgcaggaaa ctcctatatc
      901 accaatggat ctgcagcagc aatagtaaaa gtaaacacaa ccggtacagt tgtttggaac
      961 aacgcgaacc cgggaggtat tttaacgctt actgaattct ggaacattgc attcaattgt
     1021 gatcagacca tgttggttgt tggcggaacc ggaagtgttg gatttttacc tacgcaaccg
     1081 cttccctggg tttacaacat caatatgaac accggaaatg tgattaacag tttacaggta
     1141 gcaggtggag gtgcgattct gaattcacag gaagtccgtt cgatcaccgc aacaaaagat
     1201 gataaatatt attttctcac tcacgatact atcggttata tcagttccga tttaagcctt
     1261 tgtcccggac aaaacggtcc gttcaaaatt gaaaacggcg gctacaatct ctcctataaa
     1321 tgtgaaaact tccgttacga caacacagga ctggaagcac ttgcctatta tggaaatttc
     1381 gtgtttgtaa accgcggaac acgacttgat aaaagagatc ttgcaaccgg agcaattgtt
     1441 gcaaccaccc ctattcccgg aggtggattc acagcctctc tagggcaaaa ccaggctcat
     1501 aacagcggta ttgcaattga taactgcgga aacatttttg taggatctaa aaccggcttg
     1561 tataagttcg atcaaagcct cggtcaaaca ggattttttg caacaacttt taatgtttac
     1621 gacgtagata ttacttctac cggcgaagtt gttgttgcag gatcaaccgg tgatgccaat
     1681 tcaaactcac gtacaggttc tgttcaatct gtagcagcaa cagcatgtgc acaacaatct
     1741 actacatgct gcgatccaac ggtatgtaat gttccttcgc tttgtacatc gaacagccct
     1801 ataactttat ctgctggttc tcctggcgga acatggagta tttctccggc aactccggct
     1861 ttaaacacaa gcactggagt attcactcct gcatctgcag ctccgggaac ttatactgtt
     1921 acctatacac tggcttgcgg aaatgataca caaacaataa ctgttgcgaa ttgcagcgca
     1981 ttaactgtat gtcaggaaag caacggaacg tacactgttt caggtggagc aggcggacca
     2041 tttacatggc agagtggtgc aatggttact ccatgtgtat ccggatttag cttttgtaat
     2101 gcaacaccat tttcagcaaa cggaacaccg gtattaacat ggacaacctt taccacagga
     2161 actaacgtta caattggtgg tggcatcaca caaattcagg ttcttgacgg atttggaaac
     2221 agtgtaattt acaatcctgt ttcttccatt cctgcatgtg ctgcttgtcc aaccataact
     2281 gtaaccacaa gcaatgttca aaacgtagct tgtttcggac aatcaacagg cagctttact
     2341 gttgctgctt cgggtggtgc aggtccttat acctatacta ttctaaacgg cgcaacaacc
     2401 gttgcatcat tccccaatgt agcaggatca caaaacgtaa ccggattacc ggcaggtaca
     2461 tacactatca atgtactaga cgcagcaagt tgtccgggca cagtgaatgt aaccattaca
     2521 caacctgcaa ctgcagtaag tgttagcgca acatctactc ccgcaacctg tggaagtaat
     2581 aacggaacag taacaggaag cggaacaggc ggaacaggtg ccatcactta ttcctggaac
     2641 acaacaccaa ttcaaaatac tgcaacagca accggactag gtgcaggaac ttatactgta
     2701 actgctactg atgcaaacgg atgtcaggca actgctactg caacgataac ttcctccggt
     2761 gggccaacag taaccactac gcaagtcaat gtatcctgct ttggaggaaa caatgctact
     2821 gctacagcct ctgcaactgg cggaacagga acgataacct atgtgtggag tacaactcct
     2881 gcacaaactg cagccactgc aacaggatta acttccggaa cttatacagt aactgcaact
     2941 gatggtggtg gatgttcagg ttcagcaagt gttaccatta cgcaacctgc aactgcagtt
     3001 agtgtaactg caaatgcaac cgcagcaacc tgtggtttat cagatggaac tgcaactgca
     3061 aatggaacgg gtggaacggg cgctatttct tactcatgga atacaagtcc ggtacaaact
     3121 accgcaacag caacaggtct cgctgcagga ccatacatcg taactgcaac agatgcaaac
     3181 ggatgtactg caacagcaac agcaacggta aacaataccg gagcgcctac cattaccatc
     3241 agcagtcaga acaatcctgc ctgtaataac ggcaccaatg gaactgcaac ggtatccgca
     3301 agtggtggaa caggtacact gaattattcc tggaacacca ctcctgtaca aacaggtgca
     3361 actgcgaata atcttggagc aggaacctat actgccactg taacggatgg aagcggatgc
     3421 tctgcttctg ttgttgtaac aattacaaat ccgccggcaa ttacagcaag tgtatctgta
     3481 accaacgcca actgtgcagc cagcgatgga tccgcaacgg tgaatgccag cggtggaaca
     3541 ggaacattat cctatagctg gaatacaacg cctgtacaaa caacagccag cattactgcc
     3601 cttgcacccg gcgcttacag tgtaacggtt actgatgcca acggatgctc aacaacaacc
     3661 gggggcactg ttgcaacagt aggaacagca aatatcgatg caggaaatga cgtgttgatt
     3721 ccattaggtg caagcacagt attaaacgct acaggaggaa cagtttacac atggacacct
     3781 gcaacaggat taagttgtac caattgtgca tcaccaactg catcaccaac tacaaccaca
     3841 acctatattg ttagcggcac cgatgcaaac ggttgtttcg gatccgatac cgttgttgtt
     3901 tttgttgaac tgccttgcgg agaaattttt gttccgaacg cattctcacc aaacggagat
     3961 aatgaaaacg atgtactgcg gatctatggc aattgcatca acgaactcta ttttgtcatc
     4021 ttcgatcgtt ggggtgaaaa agtgtttatc accaccaatc cggctgaagt atgggatgga
     4081 actattcgcg acaaagcgat gaataacgct gtattctttt actacctctc tgcaacgctc
     4141 aacaatggtg atgttgtaga aaaatcagga aacattacat taatcaaata agcagtccta
     4201 tgaagaacaa gattctatca cttactctcg ggctggttgc aggagtggtc tctgcacaag
     4261 acatccactt ctcccaattc tatatggcac ccattcagct taatcccggt gctgccggaa
     4321 cggaagcaga tattagagtg catcttaatt acaaggagca atggaaaagt gttgcttccc
     4381 cttacaaaac cattggaggc tcttacgata tgcgttttaa gaaaacgaaa agcggatatt
     4441 ttgggggagg tattttcttc tactctgatc gtgcaggtga tgcacgcatg ggtgttgcac
     4501 agggaaatct gaatctggct tatcacctgg taattggtca aggtcaaacc ttgggattag
     4561 gtgtaatggg aggttatgcg caacgttctg taaataccac caacatgcaa tggggaaatc
     4621 agtacgatgg aatggcctat aattcttctc ttcctgcagg a
//
LOCUS       DAOMYG010000261         4649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6447_length_4649_cov_7.801045, whole genome shotgun sequence.
ACCESSION   DAOMYG010000261 DAOMYG010000000
VERSION     DAOMYG010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6447_length_4649_cov_7.801045"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PK610_09035"
     CDS             <1..204
                     /locus_tag="PK610_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36120.1"
                     /translation="TFPARRSPTKRSPIDEMNFFMTIEFNGWLIVSNLMENHFGLAVI
                     NKDLTGCSQDSPRYIKMNAAFIG"
     gene            complement(194..640)
                     /locus_tag="PK610_09040"
     CDS             complement(194..640)
                     /locus_tag="PK610_09040"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRJ36121.1"
                     /translation="MRTIVITLLFSLGWGLGSAQTTKPATVKNTKTKTEKASAQVLPA
                     KPVLAIENNDADLGDRKEGSDVEYVFIIRNNGGEPLILSEVVRTCGCTELEWTKEPIM
                     PGKTGTVKIKYDSKRVGPFNKSVIVNSNDPDQPQTTLRFKGVIVTQ"
     gene            816..3458
                     /locus_tag="PK610_09045"
     CDS             816..3458
                     /locus_tag="PK610_09045"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRJ36122.1"
                     /translation="MEISKKYEPVAIEAKWYKEWLDGGYFKSIPDGRESYTVLIPPPN
                     VTGVLHMGHMLNNTIQDVLIRRARMLGKNACWIPGTDHASIATEAKVVNKLRKEGINK
                     ADLSREEFLQHAWDWTEKHGGIILEQLKKLGASCDWDRTRFTMDEHYYNSVIDVFIDL
                     YNKGKIYRGVRMINWDPEALTAVSDEEVQHKEVHSKLFFVRYKVDGSEDEWVTIATTR
                     PETILADTAVCVHPDDERYKNVIGKQVIIPIVNRKVPVIADTYIDIEFGTGCLKVTPA
                     HDINDYELSRKHNLQVIDILNDNGTLNHHAMHYAGIDRFKVRKAIAKELDEQGYLVKV
                     EDHINKVGFSERTDAVIEPKLSLQWFVSMKELSEPALESVMNDTIRFFPPKFKNTYRH
                     WMENIKDWCISRQLWWGHRIPAWYYGTSPDDFVVARTLDDALAKAHGKSGNKNLKPED
                     LRQDEDVLDTWFSSWLWPISVFDTDVYTKGTPNEELRYYYPTSDLVTAPEIMFFWVAR
                     MIIAGYEYMGEFPFRNVYYTGIVRDKLGRKMSKSLGNSPDPIELIGKYGADGVRVGML
                     LSSPAGNDLLFDSSLCEQGRNFSNKVWNAFRLVSGWRISEDEQPEGSATAIKWFEARF
                     HQALAEINDHFEKFRMSDALMSMYKLVWDDFCSWYLEMIKPGFEAPIDTRTHEATIGF
                     FELLLKTMHPFMPFLSEELWHQIRERKKEDMLIVAKWPEYKAPDQKILNQFAFAEQVI
                     TQVRAIRNEKNIAPKVKLQLNIVARETSVDRSFDEIIARLCNISSLDYVQEKVSNAYT
                     FVVKSNEYFIPFNESIDVKAEIESLKKELDYTKGFLNSVMKKLSNERFVGSAPVDVVD
                     MERRKLADAEAKIKVLENKLASLN"
     gene            3467..3895
                     /locus_tag="PK610_09050"
     CDS             3467..3895
                     /locus_tag="PK610_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36123.1"
                     /translation="MKKLILIASIAFFALPAIAQDANCLPESVYPDSAFNTETARVIR
                     NKFSMSNSPIYKGTYLRQVAISTFQENTKDGKVVSRDISFTVAKKNEKGKCVLMKVKI
                     RQDALLDNKGWGKPYVEGAMPQSGTVDCDCVMKAKDWYRA"
     gene            complement(3896..>4649)
                     /locus_tag="PK610_09055"
     CDS             complement(3896..>4649)
                     /locus_tag="PK610_09055"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HRJ36124.1"
                     /translation="GTEEENSVSFAITSANEPVQYTYKINATYPHDVQAYTQGLEFSN
                     GSLFEGTGLDGRSELRLVDRITGKVIKSVPVESNYFGEGITIMEDKIFQLTYRARTGY
                     VYDKKSFEKIREFNYETEGWGMTNDGQHLIYSDGTHQLYFLNPENFQKIRTVEVYDNG
                     GPQNYINELEYINGEIWANIYQSDLIIRIDPMSGRVTGKIDMRGLLAPEDLHPSIDVL
                     NGIAYDKAAGKIYVTGKNYPKLWEVSILPKAQ"
BASE COUNT         1317 a          908 c         1054 g         1370 t
ORIGIN      
        1 acatttcctg caaggagaag tcctacaaaa agaagtccga ttgatgagat gaattttttc
       61 atgacaattg aatttaatgg ttggttgata gtttcaaatt taatggagaa tcattttggg
      121 cttgccgtca ttaacaaaga tttaacaggg tgctctcagg attcacctag gtatatcaaa
      181 atgaatgccg cttttattgg gtaacaatta cccctttgaa acgcagtgtt gtctggggtt
      241 ggtccggatc gttgctgttc acaataaccg atttattgaa aggcccaaca cgcttggagt
      301 cgtacttgat ttttactgtt cccgtttttc cgggcatgat cggttccttg gtccattcga
      361 gctccgtgca tccgcatgta cgaactactt cgcttaaaat gaggggctcc cctccattgt
      421 tgcggattat gaaaacatac tcaacatcag atccttcctt acggtcgccc aggtcggcat
      481 cgttattctc aatagccaaa accggttttg caggcagtac ttgtgcactc gctttttcgg
      541 tcttggtctt tgtgtttttc acggtggcag gctttgtggt ttgggcagat cccagtcccc
      601 agccaaggga aaaaagcagt gtaattacaa tcgttctcat agtttgattt atttgttttt
      661 cctgaattaa ctgaaccaaa tctctcaaaa tctattccgg gttcagggac attaacattt
      721 ggctaacgaa aattaacgaa cactttattc gaatggggtt tgcgctttta gtcctcgcac
      781 aaaatcatac ctttgcgcct tgacagtggt acattatgga gatttcgaaa aaatacgaac
      841 ccgttgcaat tgaggcgaaa tggtacaaag aatggctgga tggcggttat tttaaatcca
      901 tcccggatgg acgtgaatcc tataccgtat taattcctcc acccaatgtt accggtgttc
      961 tgcacatggg acatatgctg aacaatacca ttcaggatgt tttgatacga agggcgcgca
     1021 tgttgggtaa gaatgcctgc tggataccag gaaccgacca cgcatcaatt gctactgagg
     1081 ccaaagtagt gaacaaatta cgcaaggaag gaatcaataa agccgattta agccgtgaag
     1141 aattcttgca acatgcctgg gactggaccg aaaaacacgg tggaattatt ctggagcaat
     1201 taaaaaaact tggagcttcc tgcgattggg acagaactcg ttttacaatg gatgaacatt
     1261 attacaacag cgtaatagat gtttttattg atctgtataa caaaggcaaa atttaccgtg
     1321 gtgttcgaat gatcaattgg gatcctgaag cattaactgc ggtatctgat gaagaagtgc
     1381 agcataagga agtccattcg aaattgtttt ttgtacgcta caaagtggat ggcagcgaag
     1441 acgagtgggt gacaattgca acaacacgtc ctgaaacaat tcttgccgat accgctgttt
     1501 gcgtgcatcc cgatgatgaa cgttacaaaa atgtgatcgg taagcaggtt ataattccaa
     1561 tagtaaaccg taaggttccg gttattgctg atacctacat cgatattgaa ttcggaacag
     1621 gatgtttaaa agttactccg gctcacgata tcaacgacta tgaattatca cgtaagcaca
     1681 atctgcaggt aatcgatatc cttaacgata acggtacact gaatcatcat gccatgcatt
     1741 atgcaggtat cgatcgtttt aaagtgcgta aagcaatcgc caaagaattg gatgagcagg
     1801 gatatcttgt aaaagttgaa gatcacatca acaaagttgg tttctctgaa agaacggatg
     1861 cagtaattga gcctaaactt tcgctgcagt ggtttgtgag tatgaaggaa ctttcggaac
     1921 ctgcgcttga aagtgtaatg aacgatacga ttcggttttt tcctccgaag tttaaaaata
     1981 cgtaccgcca ttggatggag aacatcaagg actggtgtat ctcacggcaa ttgtggtggg
     2041 gacatcgtat tcccgcatgg tattacggaa cctcaccgga tgattttgtt gttgcacgta
     2101 cacttgatga tgcacttgct aaagcacatg gaaaatccgg aaacaaaaat ctgaaaccgg
     2161 aagacctgcg tcaggacgaa gatgttctgg atacctggtt ttcctcatgg ttgtggccga
     2221 tttctgtatt cgatacagat gtctatacca aaggcacacc caatgaagag ctacggtatt
     2281 attatcccac aagtgatctt gtaactgctc cggaaatcat gtttttctgg gtagcacgaa
     2341 tgatcattgc aggttatgaa tacatgggtg agtttccttt ccgtaacgtg tattatacag
     2401 gtattgttag ggataaactc gggcgaaaaa tgtcgaagtc attggggaac tctccggatc
     2461 ccattgaact catcggtaaa tacggagcag atggtgttcg tgtgggtatg ttgctaagct
     2521 cacctgccgg taacgatttg ttgttcgata gttccttgtg tgagcaggga cgtaattttt
     2581 ccaataaagt ttggaatgca ttccgtctcg tttccggatg gagaatcagc gaagacgagc
     2641 aaccggaagg cagtgcaacg gctataaagt ggtttgaggc gcgtttccat caggcattgg
     2701 cagagatcaa tgatcatttt gaaaaattca gaatgtcgga cgcattgatg tcgatgtaca
     2761 aattggtttg ggatgatttt tgctcttggt atcttgaaat gatcaagcca ggatttgaag
     2821 ctcctatcga tacgcgaacg cacgaagcaa ccatcggatt tttcgaatta cttctaaaaa
     2881 cgatgcatcc gtttatgcct ttcctgagcg aggaactctg gcatcagatt cgtgagcgca
     2941 aaaaagaaga tatgctcatt gttgcaaaat ggcccgaata caaagctccc gatcaaaaaa
     3001 ttctgaatca gtttgcattt gccgaacagg tcattacaca agttagggca attcgaaatg
     3061 agaagaatat cgcacctaaa gtaaaactcc aactgaacat tgttgctcgg gagacttctg
     3121 tcgatcgttc tttcgatgag atcattgcgc gtttgtgtaa tatcagttca ttggattatg
     3181 ttcaggaaaa agtttcaaat gcatatacgt ttgttgtgaa gtcgaacgaa tattttattc
     3241 cgttcaacga aagcatcgat gtaaaagcag aaatagaatc cctcaaaaag gaattggatt
     3301 acaccaaagg atttctgaat agcgtaatga aaaaactgag taatgagcga tttgttggga
     3361 gtgcacctgt tgatgttgta gatatggagc gccgcaagct tgccgatgct gaagcgaaaa
     3421 ttaaagtact tgaaaataaa cttgcatcac ttaactaaat gaatccatga aaaaattaat
     3481 tctaatcgca tcgatcgctt ttttcgcact tcctgcaatt gctcaggatg ccaattgcct
     3541 gcctgaaagt gtatacccgg attctgcttt taatactgaa acagcccgcg tgattcgcaa
     3601 taaattcagt atgtctaatt cacctatcta taaggggaca tatcttcgtc aggtggctat
     3661 ttcaactttt caggaaaaca ctaaagatgg aaaagttgtg agtcgcgata tcagttttac
     3721 tgtggcgaag aagaatgaaa aaggaaaatg tgtactgatg aaagttaaaa ttcgtcagga
     3781 tgctttgctt gataataaag gctggggcaa accttatgtt gaaggtgcaa tgccgcaatc
     3841 cggtactgtg gattgcgact gtgtaatgaa agccaaagat tggtaccgcg cctgattatt
     3901 gtgctttcgg cagaatgctg acttcccaaa gtttcgggta gttttttcct gttacataaa
     3961 ttttaccggc ggctttgtcg taggcaattc cattcaacac atcgattgaa ggatgaagat
     4021 cttcgggcgc aagtaagccc cgcatatcga ttttacctgt taccctcccg ctcatcggat
     4081 cgattctaat gatcaggtcc gattggtaaa tattggccca gatctcgccg ttaatgtact
     4141 ccagttcgtt aatgtaattt tgcggaccac cattgtcgta gacttctact gtacggattt
     4201 tttgaaaatt ttcggggttg aggaaataca attgatgggt gccgtcgctg taaattaagt
     4261 gctgtccgtc gttggtcatg ccccatcctt cagtttcgta attgaattcc cggatttttt
     4321 cgaaactctt tttatcgtaa acataacccg ttctcgctct gtaagtgagt tgaaaaattt
     4381 tgtcttccat gattgtaatt ccttcaccaa agtaattgga ttcaacagga acacttttga
     4441 tcacttttcc ggtaatgcga tctacaagtc gtaattcgct tcttccgtca agaccagtgc
     4501 cctcaaataa agagccatta ctgaattcga gtccttgtgt ataggcttgc acatcgtgtg
     4561 gataagtggc gttgattttg taggtgtatt gaaccggttc gttggcggat gttatggcaa
     4621 aacttacgct attctcttct tcggtgccg
//
LOCUS       DAOMYG010000262         4623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6504_length_4623_cov_7.665061, whole genome shotgun sequence.
ACCESSION   DAOMYG010000262 DAOMYG010000000
VERSION     DAOMYG010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6504_length_4623_cov_7.665061"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..1450
                     /locus_tag="PK610_09060"
     CDS             68..1450
                     /locus_tag="PK610_09060"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRJ36125.1"
                     /translation="MRKIAPRIYSSNFNDNPDVIIKRNLFTILGKLYPGAILSHRSAF
                     EFQPTTSGQIFLTYSYTKKISLGSITLRFLEGYPPIEGDLPFSGELYVSQPERAMLEN
                     LQVSRQSGPQSKSLSTRDLEEKLEAIIRTRGEQGLNEFRDRAKSISVQLEMNREFAIL
                     NKLISALLSTHPSRILKSPIAMARAIGNPYDATRIALFEKLFIALNKEEFILIPDKNK
                     SINAFRDFAFFEAYFSNYIEGTEFELEDARKIISTDTPMQNRNEDSHDILGTFKIVSN
                     KAEMNLIPRNPDQLIEMLLYRHKVLLSARTSKNPGLFKDKNNRAGETYFVDHGLVKGT
                     LKKGYEYYQALKHPFSRAVYMMFMVSEIHPFLDGNGRIARVMMNAELTTADQAKIIIP
                     TVYRDDYVGALRKLTRQAEPSAYIRMMLRAWKFSSTIPGENFDIMRSYLEECNAFKDH
                     DQAKLKFRFP"
     gene            1530..2150
                     /locus_tag="PK610_09065"
     CDS             1530..2150
                     /locus_tag="PK610_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRJ36126.1"
                     /translation="MTTTIETYLLEGCGRCSLGGTPQCKVHTWSKELKALRSIVLDCG
                     LTETVKWGVPCYTHGKANVAIVAAFKNYASISFFKGTLLSDADKLLTAPGDNSQATRQ
                     IRFTSVKDIVKHEQEIRNYIFEAIEIEKAGLKVDFKAKPAPEYPEELTAIFKKKPTFK
                     KAFESLTPGRQRGYLLFFSQAKQSATKISRIEKYLPKIMDGKGMMD"
     gene            complement(2472..>4623)
                     /locus_tag="PK610_09070"
     CDS             complement(2472..>4623)
                     /locus_tag="PK610_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ36127.1"
                     /translation="KEKKVVLMLLTAVNVFTAKAQDKDSSKLLQEVIVSASRIEEDPS
                     ATTRSVTVIGSDEIAKMAYRNVGELLTAQEGIYVVGANQNPGMIQTVFMRGTNSNQTI
                     ILIDGVRITDPSSVNNAVDLSELSLANVERIEIVRGSHSTLYGSSAIGGVINIITKKG
                     KQQGLSGSVLAEGGLFAPEGKSLNAQVFLNYRDTSSGFYANASAYRMNSTGIDATLDT
                     VTTAGVFKNRDRDGFVKTDISARIGIDKKKFSAWTSLRLHDQQSDIDRGAFNDDENYT
                     LDFKRKAISAGIDYKFNEKMRLSYLGGLSMMERYAVNDSSVIDALGNTDQTYSDSRNT
                     GGLMSHDLQFTYLSDKIRFIAGASFYQETMNQESYYINTAWMYESKSNLDSLNLHINT
                     ISAFSQLRLNGSLINEKAKNFDLTMGGRYISNSSFGTAFTGEINPSLKIGEKDLLYLS
                     YSTGFNAPSLYQLYAPDDFYGYGITLGNTGLNAEISRSFEAGLKHRISNTSLMTVSWF
                     SNRTENMIDYVNLWNKNSSIDSLGWMDGVGATYVNVGTLYSNGVEISLRSELSEKVYV
                     IANASLVQGKIVLLSKGIDTAQTKGHHVQLYSSGAFITGETEQLGLSRRPTTARITLG
                     WKPVKGLDLSTTLRMAGARSDLYYDYSIYPSGGLSTLLVKDYTLLDINASWKHNSGIG
                     ATLRIENIFDTQYAEINGFRTRGRGVYFGVRYVF"
BASE COUNT         1284 a         1022 c          998 g         1319 t
ORIGIN      
        1 aggttgtttt ctcaacgggc aaccgagccg caggtaaatt atttcggaat tggaaagtgc
       61 cgggaaattg aggaagatcg cgcccagaat ttacagctcg aactttaacg acaaccccga
      121 tgttatcata aaacgtaatc tgtttaccat actgggaaag ctttatcccg gtgctattct
      181 cagtcatcgt tccgcatttg agtttcagcc aacaacttcg ggtcagattt ttcttaccta
      241 ttcgtatacc aagaaaattt ctctggggag tattacactg cgatttttgg aaggttatcc
      301 gcccattgaa ggtgatctgc cattttcagg tgagctctat gtctctcaac cggaacgcgc
      361 gatgcttgaa aatttacagg ttagtcgtca gtcaggtcct caatcgaaat cgctctccac
      421 tcgtgatctg gaagaaaaac tggaagcgat tatccgcacc aggggagaac aaggattgaa
      481 tgaatttaga gacagggcaa aaagtatttc tgttcagctg gaaatgaatc gtgagtttgc
      541 tatacttaac aaactcataa gcgctttgct ttcaactcat ccttcccgga tactgaaatc
      601 tccaatagct atggctcggg caatcgggaa cccgtatgat gcaactagaa tagcattgtt
      661 cgagaaactt tttattgcat tgaataaaga agaatttatc cttattcctg ataaaaacaa
      721 gagcataaat gcgtttcgtg attttgcttt ttttgaagcc tatttttcga attacattga
      781 ggggacagaa tttgaattgg aggatgcccg aaaaataatt tctactgata ccccaatgca
      841 aaaccgaaat gaagattcac acgacattct cggaaccttt aaaattgtat ccaacaaagc
      901 agaaatgaac ctgattccgc gtaatccgga tcaattaatt gaaatgctct tgtatcgcca
      961 caaggtattg ctgagtgcga ggacatcaaa aaatcccggt ttgtttaagg acaaaaacaa
     1021 ccgtgccgga gaaacttatt ttgtcgatca tggattggta aaagggacgc taaaaaaagg
     1081 atacgagtat tatcaggctc tgaaacatcc tttttcaaga gctgtttata tgatgtttat
     1141 ggttagtgaa attcatcctt ttctggatgg aaatggacgt attgcccggg taatgatgaa
     1201 tgctgaatta acaactgctg atcaagcaaa gattatcatt cctacggttt atcgtgatga
     1261 ttatgtagga gcgcttcgta aactcacacg acaggcagaa ccttctgctt atattcgcat
     1321 gatgttgcgg gcatggaagt tcagttcaac gatcccggga gaaaattttg atataatgag
     1381 aagttacttg gaagagtgca acgcattcaa agatcatgat caggcaaaat taaaattcag
     1441 gttcccttga tggtggtgta aaatgaaaat ggttaattcg taattcaata ggtttttgta
     1501 taaatagttt tgattttgaa cccatgccaa tgaccaccac catcgaaacc tacctcctcg
     1561 aaggctgcgg acgctgctct ttgggcggaa caccccaatg caaagtgcac acctggagca
     1621 aggaactcaa ggccctgcgc agcatcgtgc tcgattgtgg tctaaccgaa accgtaaaat
     1681 ggggagtacc ctgttacaca cacggcaaag ccaatgtcgc catcgttgct gcattcaaga
     1741 attacgcctc tataagcttc tttaagggca ctttgctcag cgatgcagat aagctcctca
     1801 ctgcacccgg cgataactca caggcaacac gacaaatccg tttcacaagc gttaaggaca
     1861 tcgtaaaaca cgaacaggag attcgcaact acatcttcga agccatcgag attgaaaaag
     1921 caggattaaa agttgatttc aaagccaaac ccgctcccga atatcccgag gaacttactg
     1981 cgatcttcaa aaagaaaccc actttcaaaa aggccttcga atcactcacc cccggacggc
     2041 agcgtggtta tcttcttttc ttctcccaag caaaacaatc tgccaccaaa atttcccgca
     2101 tcgaaaaata cctccctaaa ataatggatg gaaaagggat gatggactga attaatgtgc
     2161 tagtatgcta atgtgctaat gtgcgaatat tttaatggga atcttctaat cccgatgctc
     2221 ccggtggtcg gtcgggattg attttgactg aagtcaaaat caacatgcga atagtttctg
     2281 cttcaggaaa tccccttcag gcgaaaccga cgaaagaagt tactgccaaa cgaaaaatcc
     2341 cccattcgcg cagcgcaaat gggggatttt tatttcaatt tatccttcta atcctaatcg
     2401 tagaaggatc gttcatagta tccttcgaag ctttggcgta gaaggactgc tcatcgttca
     2461 ctgttcacca atcaaaacac atacctcaca ccaaaataaa ctccccttcc tctggtacga
     2521 aaaccattga tctctgcata ctgcgtatca aaaatatttt caattctcaa tgtcgcgcca
     2581 ataccggaat tgtgtttcca ggatgcatta atatcaagca aggtgtagtc ttttacaagc
     2641 agtgtagata aaccacctga aggataaata ctgtaatcgt aatacaaatc actgcgcgct
     2701 cctgccatgc ggagggtggt gctcagatca agtcctttca ccggtttcca tccaagtgtg
     2761 atgcgtgctg ttgtaggtcg gcgggagagt ccgagttgtt ccgtttctcc agtaatgaat
     2821 gcaccgctgc tgtaaagttg aacgtggtgt cctttggttt gtgcggtgtc gattcccttg
     2881 ctgagaagaa caattttccc ttgtacaaga cttgcattgg caatgacgta cactttttca
     2941 ctcagctccg aacgcaaact gatttctacg ccgttgctgt aaagcgttcc aacattgaca
     3001 taggttgcac caactccatc catccagccc agactgtcga tggatgaatt cttgttccag
     3061 agattcacat aatcgatcat gttctcagtg cgattgctga accaggaaac ggtcatcaag
     3121 gatgtattgc tgatgcggtg tttcaatcct gcttcgaacg agcgtgagat ttcagcattt
     3181 agtccggtat ttcccaatgt gatgccgtaa ccgtaaaaat cgtcgggagc atacaattgg
     3241 taaagcgatg gtgcattgaa tccggtagag taggagaggt acagcagatc tttttctcca
     3301 atctttaatg atggattgat ctctccggta aacgcagtac caaaactact gttgctgatg
     3361 taacgtccac ccattgtgag atcgaaattt ttcgctttct cattgatcaa tgatccgttc
     3421 aatcgcaatt gtgaaaacgc agagattgta ttgatgtgca gattcaacga atcgagattg
     3481 ctcttgcttt cgtacatcca ggcagtatta atgtaatacg attcctgatt catggtttcc
     3541 tgataaaagg atgcgcctgc aatgaaacgg atcttatccg agagatatgt gaattgcaga
     3601 tcgtgcgaca tcagtcctcc tgtgtttcgg ctatcggaat acgtctgatc ggtatttccc
     3661 agtgcatcga tcacggatga atcattcaca gcataacgtt ccatcatgga taatccgccg
     3721 aggtaactca gtcgcatttt ctcgttgaat ttgtaatcga tacctgcact tatggctttg
     3781 cgtttgaaat cgagtgtgta attctcatca tcattaaacg cgcctcgatc gatatccgat
     3841 tgctgatcgt gcaaacgaag actggtccat gcactgaatt ttttcttatc aataccgatc
     3901 ctggcagata tatcggtttt tacgaatccg tctctgtcgc ggtttttaaa tacacctgct
     3961 gttgttacgg tatccagcgt tgcatcaatg ccggtcgaat tcattcggta cgctgatgca
     4021 ttagcataga atccgctgct ggtatctctg taattaagga agacttgtgc attcagactt
     4081 tttccttcgg gagcaaagag tccgccctct gcaagaacac ttccgcttag tccttgttgt
     4141 tttccttttt tggtgatgat gttgatcact cctccaatgg ctgatgaacc atacaaagtg
     4201 ctgtgtgagc cgcgaacaat ttcgatgcgc tctacattgg caagagagag ctctgaaaga
     4261 tcaacggcat tgttaaccga tgagggatcg gtgatgcgta ctccatcaat aagaataatc
     4321 gtctggttgg agttggtacc ccgcataaaa acggtttgga tcattccggg attctggttg
     4381 gcacctacca cgtaaatgcc ttcctgtgcg gtgagtaatt cgcctacatt gcggtaagcc
     4441 atttttgcga tttcatctga tccgatcacc gtaacacttc gcgtggttgc ggatggatct
     4501 tcctcgatac gtgaggcgga aacgatgact tcctgaagca gtttgctgct gtctttgtcc
     4561 tgtgcttttg cggtgaatac gtttaccgcc gttagtagca tcaggaccac ttttttctcc
     4621 ttt
//
LOCUS       DAOMYG010000263         4622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6507_length_4622_cov_7.263411, whole genome shotgun sequence.
ACCESSION   DAOMYG010000263 DAOMYG010000000
VERSION     DAOMYG010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6507_length_4622_cov_7.263411"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PK610_09075"
     CDS             complement(<1..226)
                     /locus_tag="PK610_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36128.1"
                     /translation="MLSPEEFKRLTIQQRFNRLQKDGDFVASRFYQSFNVHLFTLYGF
                     YVEIWQTISLGDVAYIEVVGNDATLREYTKD"
     gene            complement(227..3304)
                     /locus_tag="PK610_09080"
     CDS             complement(227..3304)
                     /locus_tag="PK610_09080"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRJ36129.1"
                     /translation="MRHILHFLFLFMLISFHAKSQVRIEEAAIFNLGTFTDEDGDAED
                     WIELRNMTASPVNLAGYMLSDNLNLPQKWIMPNVTIPANGFLIVFASGKDRSGPVLHT
                     NFSISPGEVITLYHPSAGLLSSLVLPNVPHPYTYGRENNGNPAILTPASPGAPNPTNG
                     YWGILGAPGFQTTGGYYSNPVQVSIGHYDTSSIIRYTTDGSEPTLLSPVYTGPITLGF
                     TNGQANRWSTIPSNPSLNYPQGSYDSLRAQTRGWVPPYGNVFKAHVIRTRAFPTYPGA
                     LPSESSTRTFIIDPLANNRFTLPVVSLVSDSTGLFGPEDGIYIYGNAIDGNYNELGDL
                     WERKAHLELYSANGDHEFSLYLGIRTHGGGGRHSTQKSLKLNLRNAYGTDQLNHLVFP
                     MKTQNKFNDLILRGPGHRPDCIPRDDLSGMLVEGLPFDYMHLRPVIVLLNGEYWGIHT
                     IKENMNADYFSNKYSIPKDEITILDKEGDVEEGDPADEFVYQAMEDFINTADMTIPAN
                     YAWVRTQMDIDNYINYQLAEIYFGNGDWPNSNIRMWRKFTGANSNVPNGHDGKWRWIF
                     FDLDGGYGGTCNNVFPGMNGLQQATLTTPLFNSYTRVLRGLLNNPDFKAQFILRYCDL
                     LNTWFRENRVRSVIQKADQVYRPEILEHTERWRYPSMSVTLADRYTEIPGMQAWDRII
                     YRLDSFASRRPTVQRTQLINFFTLSDSVRVTLNVNDTLMGRIKINSILIHPQLPGVQG
                     PTYPWKGLYIENIPMKLTAIPLPGYQFVEWLGTGITTNEITVSLNPDTTFTALFAPDP
                     TYNPAFGIRFNEVMAMNNSVIQDEFSQYDDWFELFNYSSDTVDLNGYFISRTLSQPTQ
                     FQILSGIQTTKIAPFGFKLVWADNQPVQGNFHTNFNLPASGGSLYLFAPDGITLVDSI
                     NYAAMVSDVSFGRYPDGAPEFVWFNYTTPNMSNAMTAIEELNQSMQLIPYPNPNGDEI
                     LYLNRPGNFTVYDINGKWMGDFSNASQITLSGYPKGMYLIRDDKGNSARFIRM"
     gene            complement(3391..>4622)
                     /locus_tag="PK610_09085"
     CDS             complement(3391..>4622)
                     /locus_tag="PK610_09085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ36130.1"
                     /translation="FLFEFFRYIFLETLALFSWKFKQRVNRKALQEARKLMWDESPLV
                     SIIVPGKNEGKHIFKLAQSLREQTYKNYELIIIDDGSDDATPEICKNLERNGMITLFL
                     RNDVRGGKASAANLGLRFAKGKYIIHLDADCSYDRDAIENIIIPFYFDQRIGAVGGNV
                     MVRNYKESLCTTLQAIEYTDTISVGRIVTSHLGIYRVISGAFGAFRSDVMQRIGGWDI
                     GPGLDGDITVKIRKLGYKIHFEPLAVCLTSVPNTFRKLIKQRLRWDKSLIRFRLRKHR
                     DVFLPNQSFKLGNFVSFAENVTYNLVLNIFWYGYLFDMIFNYSSQLKYIIIMNFFLYT
                     LNNYFKFILFSPFRERKHAPIHYFLIYIPLMVFYFGYLLRLVRSVAYLSEIFFKASYK
                     DPWNPAKTSRHAKEMGL"
BASE COUNT         1359 a          960 c          962 g         1341 t
ORIGIN      
        1 tgtctttggt gtactcgcgc agggttgcat cgtttcctac cacttcaata taggcaacat
       61 cgcccaatga aatggtttgc cagatctcta catagaatcc atagagcgtg aataaatgca
      121 cattgaaact ctggtaaaaa cgcgatgcca caaaatcgcc atccttttgc agacgattga
      181 accgttgctg aatggtgagt cgcttaaatt cttcaggact tagcatttac attcgtataa
      241 atcgtgccga atttcctttg tcatcacgga tcaggtacat gccttttgga taacctgaaa
      301 gtgtaatctg agatgcgttt gaaaaatcgc ccatccattt accgttaata tcataaacag
      361 taaaatttcc aggacgattc aaataaagga tctcatcgcc gttgggattg gggtagggaa
      421 tgagctgcat tgattgattc aactcttcaa tagccgtcat cgcattgctc atgtttggcg
      481 tggtgtaatt gaaccaaaca aattccggtg caccatccgg gtaccttcca aaagaaacat
      541 cacttaccat tgctgcataa ttgatactgt ccaccaaagt gattccatca ggagcaaata
      601 aatacagact tcctccggat gctggtaaat tgaaattggt gtgaaaattt ccctgaacag
      661 gctgattgtc ggcccagacc aatttgaatc caaatggtgc aattttcgtt gtttgtatac
      721 cggataaaat ctgaaactgt gtgggctgac ttaacgttcg tgaaatgaaa tatccattca
      781 gatcaacagt gtctgatgaa taattaaaca gttcgaacca atcgtcgtat tgcgaaaatt
      841 catcctgaat aacagaattg ttcattgcca ttacttcatt gaatcgaata ccgaaagcag
      901 gattgtaggt tgggtcaggt gcaaacaatg cggtgaaggt tgtatccgga tttaagctta
      961 ccgttatttc atttgttgta attcctgtcc caagccattc aacaaactga tatccgggta
     1021 atggaattgc agtgagcttc atagggatat tctcaatgta caatcctttc cagggatagg
     1081 ttggtccctg cactccggga agttgcggat gaatcaaaat actgttgatc ttaattcttc
     1141 ccatcaatgt gtcgtttaca tttagcgtaa cgcgaacact gtctgataag gtgaaaaaat
     1201 taataagctg cgttcgttgt accgttggtc gtcggcttgc aaaagaatcg agacggtaga
     1261 taattctgtc ccaggcttgc attccgggta tttctgtgta acgatctgcc aatgttaccg
     1321 acatggatgg atatctccat cgttctgtat gctcgagaat ttcgggacgg taaacctgat
     1381 ctgctttttg aatgacagaa cgtactctgt tttctctgaa ccatgtgtta agcagatcgc
     1441 aataccttaa aatgaattgg gctttaaaat ccggattgtt taagagtccc cgaagaactc
     1501 gtgtatacga attgaacaat ggggtagtaa gtgttgcctg ttgcagccca ttcattcccg
     1561 gaaaaacatt gttacaggta ccaccatagc ctccgtccag atcgaaaaag atccagcgcc
     1621 attttccatc gtgtccatta ggaacattgg aatttgctcc tgtgaatttt ctccacattc
     1681 ggatattgct gttgggccag tcgccatttc caaaatagat ctctgccaac tgataattaa
     1741 tataattatc aatatccatt tgtgttctga cccaggcata attcgcaggt attgtcatat
     1801 ctgctgtgtt aatgaaatct tccatggcct ggtacacaaa ttcatctgcc ggatcacctt
     1861 cttcaacatc gccttctttg tcgagtatgg ttatttcatc ttttggaata ctgtatttat
     1921 tggaaaaata atcggcattc atattttcct tgatggtgtg aattccccaa tactctccgt
     1981 tgagtaaaac aattaccgga cgtaaatgca tataatcaaa gggcagtcct tcaacaagca
     2041 ttcctgagag gtcatcacgt ggtatgcaat ccggacggtg tcccggtcca cgaagtatta
     2101 gatcattgaa tttgttttgc gttttcattg ggaaaaccaa atgattcaac tgatccgttc
     2161 cataagcatt acgcaaatta agtttcagac ttttttgcgt gctgtgtctt cctccgcctc
     2221 catgcgtacg aatgcccagg taaagcgaaa actcatgatc accattggca gaatacaatt
     2281 cgagatgtgc ttttcgctcc cataaatcgc caagttcatt gtagtttccg tcgatagcat
     2341 ttccgtaaat ataaattcca tcttccggcc caaacaatcc ggtgctgtcg cttaccaaag
     2401 aaacaacggg taatgtaaaa cgattgttcg caagcggatc aataataaat gtccttgttg
     2461 aactttcact gggcaaagct ccgggataag ttgggaatgc acgggttcta atgacatgcg
     2521 ccttaaagac atttccataa ggaggaaccc atccgcgtgt ttgtgcccgt agagaatcat
     2581 aacttccctg cggataattc aaggaaggat ttgatggaat agtgctccac ctgtttgctt
     2641 gtccatttgt gaaacccaat gtaataggac ctgtatacac cggagaaagt aaagtaggtt
     2701 cagatccgtc tgttgtatac ctgatgattg aactggtatc gtaatgacca atacttacct
     2761 gaacgggatt ggaatagtaa ccgcctgtag tttgaaatcc cggagctccg agtattcccc
     2821 aatagccgtt agttggattt ggcgcaccag gacttgcggg tgtgagaatg gcaggattac
     2881 cgttgttttc ccttccgtat gtataaggat gtggaacatt tggtaaaaca agcgaactca
     2941 ataaacctgc acttggatgg tataaggtga ttacttcgcc tggactgata gaaaaatttg
     3001 tatgtaatac aggtcccgat ctgtccttac cggatgcaaa aacaattaaa aagccatttg
     3061 cgggaatggt aacatttggc attatccatt tttgaggcag attcagatta tcgctaagca
     3121 tgtatcctgc aagattcaca ggagaggcgg tcatgttgcg gagctcaatc cagtcttccg
     3181 catctccgtc ttcatcggta aacgtaccaa gattgaaaat tgcagcttct tctattctta
     3241 cctgactctt cgcatggaat gagataagca tgaacagaaa cagaaaatga agaatgtgtc
     3301 gcacgaaata aaaataagaa aaattcaaat tcaattaaca agaaggaaat ggcatttcga
     3361 tcagtcggaa tgacaggttg attctgctcg ttataaaccc atttctttgg cgtgacggga
     3421 agtttttgcc ggattccatg ggtctttgta ggatgctttg aaaaagatct ctgataagta
     3481 ggccacactt ctgactaacc tgagtaagta gccaaaataa aaaaccatca gaggaatata
     3541 aatcaggaaa taatgaatgg gcgcatgttt gcgttcgcgg aatgggctaa ataaaatgaa
     3601 tttaaaatag ttgttgagcg tgtacaaaaa gaaattcatg atgatgatgt acttcaactg
     3661 ggatgaataa ttgaatatca tatcaaaaag gtatccatac caaaaaatat taaggacaag
     3721 attataggtt acattctctg caaacgaaac aaaattgccc agcttaaacg actgattggg
     3781 cagaaatacg tcgcggtgtt tacgcaaccg gaaacggatc aatgatttgt cccaacgcaa
     3841 acgttgtttt atgagttttc ggaaggtatt cggaacactt gtcaaacaaa cagcaagagg
     3901 ttcgaaatgg attttgtacc ccagttttct gattttaacg gtaatgtcgc catctaatcc
     3961 cggaccaata tcccaacctc caatacgttg cattacatct gaacgaaagg ctccgaaagc
     4021 gccagaaatt acacgataaa taccaagatg cgatgtaaca atccttccaa cagaaatagt
     4081 gtcggtgtac tcaatagcct gaagtgttgt gcagagactt tccttatagt tccgcaccat
     4141 tacattgccc ccaactgcgc caatgcgttg atcgaaataa aatggaataa tgatgttttc
     4201 aattgcatcc cggtcgtaag aacaatctgc atcgaggtga atgatgtact tgccttttgc
     4261 aaaacgtaat ccaaggttag ccgccgaagc ttttccccca cgaacatcat tgcgcaagaa
     4321 cagagtgatc attccgtttc gctccagatt cttgcaaatt tccggtgtag catcatccga
     4381 cccgtcgtcg ataatgatga gctcgtaatt tttgtaggtc tgttctcgta acgattgggc
     4441 tagtttgaaa atgtgttttc cttcgttctt tcccggtact ataatcgaca ccaaagggga
     4501 ttcatcccac atcagtttgc gtgcttcctg cagtgccttt cgattaacac gttgtttgaa
     4561 tttccatgaa aacaaagcca gcgtttctaa aaaaatgtac ctgaagaatt caaacagaaa
     4621 at
//
LOCUS       DAOMYG010000264         4611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6532_length_4611_cov_9.379939, whole genome shotgun sequence.
ACCESSION   DAOMYG010000264 DAOMYG010000000
VERSION     DAOMYG010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6532_length_4611_cov_9.379939"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..2748)
                     /locus_tag="PK610_09090"
     CDS             complement(187..2748)
                     /locus_tag="PK610_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRJ36131.1"
                     /translation="MEAKFSPRVKDVITFSREEALRLGHDYIGVEHLLLGIIREGEGT
                     AMRVLKQLGVEARDLRKELEKNLAPGNAKSANVGNLPLVKQAEKVLKITYLEARLFKS
                     SVIGTEHLLLSILKDEDNVATRSLQRFNVEYDTIKTELENMHKDSNYPKSELPNNPAD
                     DDDENAGDSFSQSQRRTDSKSKTPVLDNFGRDLTKAAEDGKLDPIVGREQEIERVCQI
                     LSRRKKNNPILIGEPGVGKSAIAEGLALRIVQRKVSRVLFNKRVVSLDLASLVAGTKY
                     RGQFEERMKAVMNELEKSPDVILFIDEIHTIIGAGGASGSLDASNMFKPALARGEIQC
                     IGATTLDEYRQYIEKDGALERRFQKVIVEPTSIDETIQILNNIKEKYEDHHNVNYTPD
                     AIDACVKLTARYITDRHLPDKAIDALDEVGSRVHLSNINVPQNILDIEKKIEDIKEEK
                     NNVVRSQKYEEAAKLRDTERQLQEQLEAAKRKWEEESKLQRVTVTEDNVAEVVAQMTG
                     IPVQRIAQKESGRLVQMADNLKGRVIGQELAVQKVVKAIQRNRAGLKDPNKPIGSFIF
                     LGPTGVGKTQLAKELAKYLFDSEEAMIRIDMSEYMEKFALSRLIGAPPGYVGYEEGGQ
                     LTEKVRRRPYSIILLDEIEKAHPDVFNLLLQALDDGQMTDSLGRKIDFRNTIIIMTSN
                     IGARQLSDFGAGVGFGTKAKEDTSERDNKGVVEAALRKAFAPEFLNRIDDVVMFNSLK
                     REDIHQIIDLELNKLFKRINELGYKIELTSDAKDQIAENGFDEKFGARPLKRAIQKYL
                     EDPLAEEIIKSNLQEGDTIKVGLDTATKDLQIQIVKGTPAPKKKPKKEEENPSED"
     gene            2983..>4611
                     /gene="gyrA"
                     /locus_tag="PK610_09095"
     CDS             2983..>4611
                     /gene="gyrA"
                     /locus_tag="PK610_09095"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRJ36132.1"
                     /translation="MAEGEKIIPINIEVEMKTAYIDYSMSVIVSRALPDVRDGLKPVH
                     RRVLFGMQELGVLSNRPHKKSARIVGEVLGKYHPHGDSSVYDTMVRMAQDWSLRYPLV
                     DGQGNFGSVDGDSPAAMRYTEARLRKIAEELLADIEKETVDFRPNFDDSLEEPTVLPA
                     RIPNLLINGSSGIAVGMATNMPPHNLTEVIDGIIAYIDNRDIDIAGLMKFVKAPDFPT
                     GGTIYGYDGVKRAFEEGRGRVVVRGKATIEEERGRNVIIVSEIPYLVNKAEMIRKTAE
                     LVEEKKIEGISDIRDESDRTGMRVVYELKRDAIPNVVLNNLYKYTDLQNSFSVLNIAL
                     VKGRPLVLNLKEMIVQFVEHRHEVIVRRTKFELRKAEERAHILEGLIIASDNIDEVIA
                     LIRASSSPDEAREKLMERFGLSEIQSRAIVEMRLRQLTGLEQDKLRGEYEDVMALIAR
                     LKNLLDNEDMRMEVIKEELREIRANYGDERRSQIEYASGDFRIEDMIADEEMVITISH
                     LGYIKRTPLREYRAQSRGGTGSRGSGTRDADFLEH"
BASE COUNT         1116 a         1039 c         1087 g         1369 t
ORIGIN      
        1 catatccttt tcgctcaaat tatttgagac cataaaaaga aaaagggttg ggactaaaga
       61 aagaaaaaac aaacccatta aaacaccttg cttccgtgaa atggatttct tgtcaaactt
      121 ttcggatctt tttcgtctac tatttctcat aaataaaaat ggtcccgatt gtacgggacc
      181 attgtattaa tcttcagaag gattttcttc ttcttttttc ggttttttct tcggcgcagg
      241 agtacctttt acgatttgta tctggagatc tttagtagct gtatcgagtc cgactttaat
      301 cgtatcaccc tcctgcagat tcgatttgat gatctcttct gccagtggat cttcgaggta
      361 tttctgaatg gcgcgcttca acggacgtgc tccgaatttc tcgtcgaatc cattttctgc
      421 aatttgatcc tttgcatcag atgtaagctc gattttgtat ccgagttcgt tgatgcgctt
      481 gaagagtttg ttcaattcaa gatcaatgat ctgatgaata tcttcacgct tgagcgagtt
      541 gaacatcact acatcgtcga tacgattcag gaattcgggt gcgaatgcct tacggagtgc
      601 tgcttccacc acacctttgt tatcgcgttc gctggtatct tctttggcct tggttccaaa
      661 tccgacccct gcaccaaaat cagaaagctg acgcgctcct atattggaag tcatgatgat
      721 aatggtattc cggaaatcga tcttgcgtcc gagactgtcg gtcatctgac catcatccaa
      781 tgcctgcaga agcagattga atacgtcggg atgtgctttt tcaatctcgt caagtagaat
      841 gatagaatag gggcgacgac gcactttttc tgttagctgt ccgccttctt cgtatcctac
      901 atatccggga ggagcaccaa tcagtcggct gagcgcaaat ttctccatgt attcgctcat
      961 atcgatgcgg atcatggctt cttcggaatc gaacaggtat ttcgccagtt cttttgccaa
     1021 ttgcgtttta ccaacaccgg taggaccaag gaaaataaat gaaccgatcg gcttgttggg
     1081 atctttcagt ccggcacggt tacgctgaat ggctttcacc actttttgta cagccagttc
     1141 ctgaccaatc acacgacctt tgaggttatc ggccatttgc acaagacgac cactttcttt
     1201 ctgtgcgata cgctgaacag gaataccggt catttgtgct accacttcgg caacattgtc
     1261 ttcggtaact gttacacgct gcagcttgga ttcttcctcc catttgcgtt tagccgcttc
     1321 gagttgttcc tgaagctgac gttcggtatc acgcagtttg gccgcttctt cgtatttctg
     1381 actacgaacc acattgtttt tctcctcctt gatgtcttcg attttcttct cgatatcgag
     1441 gatgttttgt ggaacgttga tattgctcaa gtgaacacgt gatccaactt catccagcgc
     1501 atcaattgcc ttatcgggca ggtgacggtc ggtgatgtag cgtgcagtga gttttacaca
     1561 ggcatcaatt gcatccggtg tgtagttcac gttgtggtga tcttcgtatt tctctttgat
     1621 gttattcagg atctgaatgg tctcatcgat ggaagtaggc tcaacgatca ccttttggaa
     1681 ccggcgctcc aaggctccgt ctttttcgat gtattggcgg tactcatcaa gtgtagttgc
     1741 tccaatgcat tggatctcac cacgggcaag cgcaggcttg aacatgttgg aagcatccag
     1801 cgaaccggaa gctccacctg caccaatgat ggtgtggatt tcgtcaatga aaaggataac
     1861 gtcgggcgat ttttccagct cgttcatcac ggctttcatg cgctcttcga actgaccgcg
     1921 gtacttggtt cctgcaacga gtgaagcaag atcaagagat accactcgct tgttgaacaa
     1981 cacccgggaa accttgcgct gaacgatacg caatgcgagt ccttctgcaa tggcggattt
     2041 acctacaccg ggttcaccga taaggatggg attatttttc tttctgcggc tcaggatctg
     2101 gcaaacacgc tcaatttcct gttcgcgtcc aacaatggga tcgagctttc catcctctgc
     2161 agctttggtg agatcgcgac cgaaattatc gaggaccggt gtcttcgatt tggaatcggt
     2221 gcggcgctgg ctttgcgaga aggaatcacc tgcattttca tcatcatcat ctgccgggtt
     2281 gttgggcagt tccgatttgg ggtagttgct gtctttgtgc atgttttcga gttctgtctt
     2341 gatggtatcg tattcgacat tgaagcgttg taaagaacgg gtagctacat tgtcttcgtc
     2401 tttgagaatg gaaagtaata aatgttctgt tccaatcaca ctgcttttaa ataatcgtgc
     2461 ctcaagataa gtgatcttaa ggaccttttc tgcctgtttt accaatggca ggttacctac
     2521 attggccgat tttgcattac cgggtgcaag gttcttttcc agttctttac gcagatcccg
     2581 ggcttctaca cccaattgtt tcagcacacg catggcggtt ccttctcctt cgcgtatgat
     2641 acccagaagc aaatgctcca cgccaatgta atcatgaccc aaacggagag cttcctccct
     2701 gctgaaggtg atgacgtctt ttactctcgg tgaaaatttc gcttccatag tctttttcct
     2761 tcagtgctgt ttaccggaaa gcccacaaaa taggggactt tgcgatgctt ccaaaattat
     2821 gcagtactta tgttgacacc ttgttcataa ggtgtttttt tcaacagatt tttgtttaaa
     2881 attccattct tttttttcgg tcaaaagccc cctattatgg gtaccttcgg cctccttgtt
     2941 ggggttctcc caacaatcgt gcaaaacagt taacgagaca ttatggcaga aggcgaaaag
     3001 atcataccga tcaacattga agtagaaatg aaaacagctt acatcgatta ttcgatgtcg
     3061 gttattgtat cccgtgcatt accggatgtt cgcgatggtt tgaaaccggt gcaccgcagg
     3121 gtgttatttg gaatgcagga acttggagtt ctttccaatc gtccgcataa aaaatcggcc
     3181 cgtatcgtgg gtgaggtttt gggtaaatat cacccgcatg gcgacagctc agtttacgat
     3241 accatggtgc gtatggccca ggattggtcc ttgcgttatc cgttggtgga tggccagggt
     3301 aacttcggtt cggttgacgg tgatagtcct gctgcaatgc gttataccga agctcgtctg
     3361 cgcaagattg ccgaggagtt attggccgat attgaaaagg aaacagttga ttttcgtccc
     3421 aatttcgatg attcgctcga agaacctact gtacttcctg cacgcatacc gaatttgctg
     3481 atcaacggtt cttccggtat cgcagttggt atggccacca atatgccgcc tcataatcta
     3541 actgaggtta ttgacggaat cattgcctat atcgacaacc gcgatatcga tatcgccgga
     3601 ttgatgaaat ttgtcaaagc tcccgatttc ccgacaggtg gaaccattta cggttacgac
     3661 ggcgtaaagc gtgcttttga agaagggcgc ggtagggtag ttgttcgtgg taaagctact
     3721 atagaagaag aaagaggtcg caatgtgatt atcgtttcgg agatacccta cctggtgaac
     3781 aaggcagaga tgatccgcaa aacggctgaa ttggttgagg agaaaaagat cgaaggaatt
     3841 agtgatatcc gcgatgaatc tgaccgtacc ggtatgcgcg tggtgtatga actcaagcgc
     3901 gatgctattc ctaatgttgt actgaataac ctgtacaaat acaccgatct ccagaattca
     3961 ttctcagttc tgaacattgc ccttgtaaaa ggtcgcccac tggtgctgaa cctaaaggaa
     4021 atgatcgttc aatttgttga acaccgtcat gaagtaattg ttcgccgcac caaattcgaa
     4081 ttgcgcaaag ccgaagagcg tgcacatatt ctggaagggc tcatcattgc ttctgataat
     4141 attgacgaag taattgctct aatccgtgcc tcttccagtc cggatgaagc ccgtgagaaa
     4201 ctgatggagc gttttggttt gagcgagatt cagtcccgcg caattgtaga aatgcgtctg
     4261 cgtcagctta ccggtcttga gcaggataag ctgcgtgggg aatatgaaga tgtgatggcc
     4321 ttgattgccc gtttgaaaaa tcttctggat aatgaagata tgcggatgga agtcattaag
     4381 gaagagcttc gtgagatccg tgccaattac ggagatgagc gtcgttctca aatcgaatat
     4441 gcatccggag atttccgtat tgaggatatg attgccgatg aagagatggt gattaccatt
     4501 tctcacctcg gatacattaa acgtactcca ttgcgcgagt atcgtgcaca aagcagggga
     4561 ggaacaggtt cacgcggatc cggaacacgt gatgcggatt tccttgaaca c
//
LOCUS       DAOMYG010000265         4608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6537_length_4608_cov_7.908851, whole genome shotgun sequence.
ACCESSION   DAOMYG010000265 DAOMYG010000000
VERSION     DAOMYG010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6537_length_4608_cov_7.908851"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /gene="trmD"
                     /locus_tag="PK610_09100"
     CDS             complement(<1..486)
                     /gene="trmD"
                     /locus_tag="PK610_09100"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRJ36133.1"
                     /translation="MRIDILTVLPSLLESPFADSILKRAIEKGHVEVHVHNIRDYSES
                     KHKQVDDYQYGGGAGMVLMAEPIAKCIDKLQTERKYDEIIYMTPDGKTLDQRDVNGFS
                     LLKNLLIICGHYKGIDQRIRDEYVTREISIGDYVLSGGELAAAVLCDAIIRLIPGVLS
                     DE"
     gene            complement(704..1207)
                     /locus_tag="PK610_09105"
     CDS             complement(704..1207)
                     /locus_tag="PK610_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRJ36134.1"
                     /translation="MKTLYLIRHAKSSWGDLSLSDFDRPLNERGKRNAPFMGKIIRSK
                     GIKPDLIVSSPAKRAYSTAKKIAKEIGYKTENIKTESSIYEADVRTLLKVVNELSANA
                     KTVMLFGHNPGFTDFANYLSGANISNIPTCGMVCIRFSTSKWEEISRDTGTFTEFDYP
                     KNYPEGY"
     gene            complement(1204..1440)
                     /locus_tag="PK610_09110"
     CDS             complement(1204..1440)
                     /locus_tag="PK610_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36135.1"
                     /translation="MKKSGCCPKCNSTEVYTTKGTTKRGERSSIGISSFSSVSIDLYI
                     CISCGFIEEYGEESDLKDLKKMEKLKKLFRKVQP"
     gene            complement(1473..2375)
                     /locus_tag="PK610_09115"
     CDS             complement(1473..2375)
                     /locus_tag="PK610_09115"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HRJ36136.1"
                     /translation="MKNISVIGAGTMGNGIAHVFAQFGYKVNLIDVSEEALKKALATI
                     GKNLDRQVAKGSLTEADKENTLKNLTTYTDLKTGVSDADLVVEAATENVDLKLKIFRD
                     MDAFAPANAILSTNTSSISITRIAAQTKRPDKVIGMHFMNPVPVMKLVEVIRGYATSD
                     EVTSAVMEMSRKLAKVPVEVNDYPGFVANKILMPMINEAIITLWQGTAGVEEIDTVMK
                     LGMAHPMGPLQLADFIGLDVCLSILRVLQEGFGEDKYAPCPLLVNMVTAGKLGVKSGE
                     GFYSWTHGTKDLVVADNFRKKVTA"
     gene            2439..2783
                     /locus_tag="PK610_09120"
     CDS             2439..2783
                     /locus_tag="PK610_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HRJ36137.1"
                     /translation="MFKTRIRWYKIGASTDAIVASVPLNGVKGINVGGKNICLVHTTE
                     GLSAVRDKCPHQGVRLSKGGCAEDGKIVCAWHRYAFDLKTGRGAGLHVDVYPIEIRDD
                     GVYLGMEYLSLF"
     gene            complement(2915..>4608)
                     /locus_tag="PK610_09125"
     CDS             complement(2915..>4608)
                     /locus_tag="PK610_09125"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HRJ36138.1"
                     /translation="LVDKKAQIKYTVISLALVLFGIFIDLNTVLYSSGDFVLTWRGLI
                     ILVAFTYTIIFLINNYLLHFPREKKEEELWSREFWMFIGSLVLVLSAVQITFSTSIPV
                     INLLFDTKMNLVEQAARNEAFNRWQAPFAVIICLLIAFGQFLKYKNTSFTEFGKKILP
                     SLVIAIVLTIALGTILQLTHYRYNLLLFTALWSVVANADYWLRFLKGKMNIAGSSIAH
                     IGFGILMLGALISQNKQTIISETPYGYNLSMLGKDLSNLTDVQIFKGDTTEMREYFVH
                     YSGTSQEKHFLRFNIDYFSKVPAQFQKGEHMKFDGNVYRCLNDHTSSGQLTSDISNWE
                     LLTDPGAELYFAAQPWSAWQAGEKLFHLQPFVQLNDMSNVAEPGTKHYFDHDIFTHIK
                     YADLRLVKEDDNMPPYEITTHENDTIGNPGFILRVAGIRSLNGEEKAQRGLDTSLSAA
                     QLNMVVYDIWDISFRYGKEVNPVFFIEENEFRSLPARSEELKTSFAIKSVKPKKDEHS
                     HTGFEHGGTEITLEITTPEFIIMHAIRFPWISILWIGCLIMAIGTGMAVVYRVRK"
BASE COUNT         1237 a          952 c         1054 g         1365 t
ORIGIN      
        1 ctcatcgctc aatactccgg gaattaaacg gataattgca tcacaaagca cagctgcagc
       61 taattctcct ccgctcagta cataatcgcc aatggaaatt tcgcgtgtaa cgtattcatc
      121 acggatgcgc tgatcaatgc ctttgtagtg cccgcagatg atcaaaaggt tttttaagag
      181 cgaaaagcca tttacatcgc gctgatcgag cgttttaccg tcgggtgtca tgtagatgat
      241 ctcgtcgtat ttgcgttcag tctgaagctt gtcgatgcat ttggcaatgg gttcggccat
      301 cagtaccatt cctgcgccac cgccatattg gtagtcgtcg acttgcttgt gcttgctttc
      361 cgaataatcg cggatgttgt gtacatggac ttcaacatgt cctttttcaa tggcacgctt
      421 gaggatggag tcggcaaacg gactttccag cagcgagggg aggacggtga gtatatcgat
      481 gcgcatgggg taaaggtagg gaatgtgcga atattctaat gtgggaatgt gggaatcccg
      541 acgctacggt cgggatgatt cttcctttgg tcgaatcatg tgcggatata cgtttgaaga
      601 attgatatcc gtgataagga ttgagggtgg tttcttatta actcttcttc ccttgatcct
      661 taatggtgac agatggaggg aaatatggat gaatcgaaag tcatcaatac ccttcagggt
      721 aatttttggg gtaatcgaat tctgtaaatg taccagtgtc gcggctgatc tcttcccatt
      781 tggatgtgct gaaccggatg cataccattc cgcaggttgg gatattggag atattggcgc
      841 ctgagaggta attggcaaaa tcagtaaaac cgggattgtg tccgaacagc attaccgttt
      901 ttgcatttgc agagagttca ttgaccactt ttagtaaggt gcgcacatct gcttcgtaga
      961 tggaagattc ggttttgatg ttttctgttt tataaccgat ttcttttgcg atttttttgg
     1021 cagtggagta ggcgcgcttg gcaggactgc ttacgatcaa atccggtttt attcctttac
     1081 tgcgaatgat ttttcccatg aaaggcgcat tgcgttttcc cctttcgttc agtggtcggt
     1141 cgaaatcgct aaggcttaag tcgccccatg aagatttggc gtgacgaatg aggtaaagcg
     1201 ttttcatggc tgaaccttcc ggaaaagttt cttcagtttt tccatttttt tcaggtcttt
     1261 cagatcgctt tcctctccat attcttcaat gaagccacag gaaatgcaga tgtagaggtc
     1321 aatcgaaaca gacgaaaagg aactaatgcc aatggatgag cgttcaccac gtttggttgt
     1381 tcctttagtc gtgtacactt cggtagagtt gcatttcgga caacatcctg attttttcat
     1441 aggagaaaaa agacgttcgt gtcaaccgaa aattatgcgg ttactttctt gcggaaatta
     1501 tctgcaacaa cgagatcttt tgtgccgtgt gtccaggaat agaatccttc tcctgatttc
     1561 acaccgagtt ttccggctgt aaccatgttt accagcaatg gacaaggcgc atatttatct
     1621 tctccgaaac cttcctgcag tacgcggagg atggagagac aaacatcgag tccgataaaa
     1681 tcggctagtt gcagtggtcc catgggatgc gccattccca gtttcattac ggtatcaatt
     1741 tcctcaacac ccgccgtacc ttgccagagg gtaatgatgg cttcgttgat cataggcatg
     1801 agaatcttat tggcaacaaa gccgggatag tcgtttactt ctacaggaac cttagccagt
     1861 ttgcgcgaca tttccataac ggctgaagtc acttcatcgc ttgtggcata tccgcgaatg
     1921 acttccacca gtttcattac cggaacggga ttcatgaagt gcataccgat caccttatca
     1981 ggacgcttgg tttgtgctgc aatacgggta atggaaatgg acgaagtgtt ggtggaaaga
     2041 atagcatttg caggtgcaaa ggcatccatg tcgcggaaga ttttcagttt gagatctaca
     2101 ttctcagtag cggcttcaac aacaagatct gcatcggaaa ctccggtttt aagatcggta
     2161 taggtagtga ggtttttgag tgtgttttcc ttgtcggcct cagtaagtga tcctttcgca
     2221 acctgacgat cgaggttctt accaatagtg gccagtgctt ttttgagtgc ttcttccgaa
     2281 acatcgatca gattgacttt atacccgaat tgagcaaata cgtgagcgat accatttccc
     2341 atggttcctg ctccgatcac agaaatgttt ttcatcttgg ttgattttgt ggctctaata
     2401 tagtgggaaa aagcacccgt tgcgctaaat ttaggccagt gttcaaaacc cgtattcgct
     2461 ggtataaaat tggagcttcc acagatgcaa ttgttgcttc tgttccactc aatggtgtta
     2521 aaggaattaa cgtaggtgga aaaaatatct gcctggttca caccaccgaa ggattatcgg
     2581 cagttcgcga taaatgtccg caccagggtg ttcgcttaag caaaggcggc tgcgcagagg
     2641 acggtaaaat tgtttgtgcc tggcaccgtt atgctttcga tctcaaaaca ggtcggggtg
     2701 caggattgca tgtcgatgta tatcccatcg aaatacggga tgatggagtt tatctgggaa
     2761 tggaatatct cagcttgttt tagaaaacag aatcttgagt tccgattgca atggtactaa
     2821 cgaatagatt ttcgttcacc ttgctggaat aggttaggag ttatcagtta ttagttagga
     2881 gtgaagagtc cattcactgt ttaccgttca cccttcactt cctcacccga tacaccactg
     2941 ccattcctgt accaattgcc atgatgagac aaccgatcca caggatggaa atccatggaa
     3001 aacgaatggc atgcattata ataaattccg gtgttgtaat ttcaagggta atttccgttc
     3061 caccgtgttc aaatccggta tgggaatgct catctttttt gggttttaca ctcttaattg
     3121 caaatgaagt cttcaattct tcagaacgtg caggaaggga acggaattca ttttcctcta
     3181 taaagaacac cggatttact tcttttccat aacggaaact tatgtcccaa atatcataca
     3241 cgaccatatt caattgtgca gcagaaagtg aggtgtcgag tcctcgttgg gctttctctt
     3301 ctccgtttaa tgaacgaata cccgcaacgc gcaaaatgaa acctggattt ccaatggtgt
     3361 cgttttcatg ggtggttatt tcataaggag gcatgttgtc gtcctcttta accaaacgca
     3421 gatcggcata tttgatgtgc gtaaaaatat catgatcgaa ataatgtttt gttccgggtt
     3481 cggcaacatt actcatgtcg tttaactgta caaatggttg gagatgaaat aatttttcac
     3541 cggcttgcca tgccgaccag ggttgtgcgg caaaatacaa ttcagcaccc ggatcggtaa
     3601 gtagttccca attcgaaatg tctgaagtta gttgtcctga cgaagtgtga tcgtttaaac
     3661 accgatacac atttccatcg aacttcatgt gttcaccttt ttgaaattgc gcaggaactt
     3721 tagagaaata atcgatgttg aaacgtaaaa aatgtttttc ctgacttgta ccggagtaat
     3781 gcacaaagta ttccctcatt tccgtggtat cccctttgaa aatttgcacg tcggtaagat
     3841 tgctcaaatc ttttccaagc atcgacaaat tatatccgta aggtgtttct gaaataatgg
     3901 tttgcttatt ctgtgagatc aaagcaccaa gcatcagaat tccgaatcca atgtgggcaa
     3961 tggaggatcc tgcaatattc attttccctt tcaggaaacg cagccagtaa tcggcattgg
     4021 caacgaccga ccaaagggca gtgaacaata acaggttgta ccggtaatgc gtgagttgta
     4081 aaatagtacc caatgcaatc gtgagtacaa tcgcaattac aagcgaaggc aaaatctttt
     4141 tcccgaattc tgtaaaggaa gtgttcttgt atttgaggaa ttgtccgaac gcaatcagta
     4201 gacaaatgat taccgcaaat ggtgcttgcc agcggttgaa tgcttcgttt cgtgctgcct
     4261 gttctaccag attcattttg gtatcgaaga gcagattgat aacaggaata gaagtactga
     4321 aagtgatctg cactgccgat aacacgagta ccaatgaccc gatgaacatc cagaactcgc
     4381 gcgaccataa ttcttcttcc tttttctctc ttggaaaatg cagcaggtaa ttattgatca
     4441 ggaagataat ggtgtaggta aatgcaacca aaataattaa tccacgccag gtcaacacaa
     4501 aatcaccgga actgtaaaga actgtattga ggtcaataaa aattccgaaa agaacaagtg
     4561 ctaaactgat tacagtatat tttatctgtg cttttttatc aaccaaaa
//
LOCUS       DAOMYG010000266         4602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6548_length_4602_cov_10.693644, whole genome shotgun sequence.
ACCESSION   DAOMYG010000266 DAOMYG010000000
VERSION     DAOMYG010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6548_length_4602_cov_10.693644"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..792
                     /locus_tag="PK610_09130"
     CDS             154..792
                     /locus_tag="PK610_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRJ36139.1"
                     /translation="MLAAILEGLFFGFVLSFMLGPSFFLILETSIQDGIKSAMLLDLG
                     VLVSDIIYIFVALKFFSRRDEIIAHEQAITIATGVLLGCFGAYQLLTKKAPSTDAPEP
                     KEIIHTRIKSVGLFLKGFLINIINPTILLYWFGMIFIGFSKNNFDDSQMIVFLGSILA
                     AFFSIDVLKIIGARQLKKAITPALMRHLNRAIGVILVVFGAVLFIKGLHIFH"
     gene            931..2619
                     /locus_tag="PK610_09135"
     CDS             931..2619
                     /locus_tag="PK610_09135"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36140.1"
                     /translation="MRLTTILFLLVVLSSPVVGQEATTPVKKTNEVERTYWDKQKKKV
                     RSEGRYRTDGFSGIGLEEGEWKYWYENGQMEGIIEFSKGQLNGRLIKYYENGKKMQEG
                     WFKMGVQDSLSSFWYNNGQLRESGSYIMGKKNGEWKYYNHTGKIILEERHENGKEFVV
                     NYWDRNGKQTITSGNGKMFVYASDNLVKEEYTYKDGLRHGACYEKNALGRVIVQGEYN
                     LGKKAGTWIFRYADGTKKREVSYKDDWMEGPFKEYMGGDSILVQGTYKAGKKDGIWEW
                     YFENGTLDMKGGFADDLQEGEWYFGYNNGKINYKGSFTAGKREGKWEYYYPEGQAYMT
                     GHFKNDVKHGPWITWFESGKKLSEGEFSEGKEQGVWTSWYDSGGTKDEGSFEKGLMNG
                     VWKGWFPNGKLNYQGSYTMGHRSGHWTYYFSNGKKRDEGDFKVISRSKSLEKNSPNAS
                     SYQESVRHGKWASFDERGEKTAEGSYYEGEMDGRWLFYQPGEIMLAQEINYKKGVLDG
                     VSISYDKKGKKISEINYKDGAPHGEYKTWDSKGNLSKHLIYKNGKVVKDKLPKK"
     gene            2750..3217
                     /gene="rimP"
                     /locus_tag="PK610_09140"
     CDS             2750..3217
                     /gene="rimP"
                     /locus_tag="PK610_09140"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HRJ36141.1"
                     /translation="MINESNIRPIVNDVLQGTECFLVELVVAPGAKILVELDSPSGVT
                     IEQLRKISRAIENQLDREKEDFELTVSSPGLEKPFRVFEQYLKNVGRSLTVDLNDGRK
                     LEGKLEEATEKGIVLESSSREKVEGKKSKQDVVRRHEVGMNEIKQAKVLITFK"
     gene            3237..4472
                     /gene="nusA"
                     /locus_tag="PK610_09145"
     CDS             3237..4472
                     /gene="nusA"
                     /locus_tag="PK610_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRJ36142.1"
                     /translation="MNGLNLIESFQDFKEFKNIDRVTMMSILEDVFRGMLKKKYGTDE
                     HIDIILNVEKGDLEIWMNREIVRDEELEDEGTQIAYSEAVKIEPDFEIGEEVSEKVEI
                     DDFGRRNILALRQNLISRVLELEKDNVYKKYKERIGEIMTGEVYQIWKKEILILDDEG
                     NELILPKSEQIPSDFFKKGDSIRAVVYKVDLRNGTPVVILSRTSPVFLERLFELEVPE
                     VFDGLITIKKIVREPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDV
                     INYTNNNQLLITRALSPAKITSVSLDEETKRAEVYLKPDQVSLAIGKGGHNIKLASKL
                     TGYEIDVYREGAEDIDDVDLDEFTDEIDAWILDELKSIGCDTARSVLDLSVEDLVKRT
                     DLEEETINEVVRILKSEFE"
BASE COUNT         1442 a          939 c         1117 g         1104 t
ORIGIN      
        1 agtcacaaag cggcggataa catgttgcat gcattacctg ccatgctcga tcatttttcg
       61 aaaaagggat ttcgcttcaa cgcaattccc tgatatccat aagcctccaa atttcattag
      121 aatagtatct ttatcggctc aaaacaattg cctgtgctag cagccatact ggagggacta
      181 ttttttggct ttgttctcag ctttatgctg ggtcctagtt tttttctcat tcttgaaacg
      241 agtattcagg atggaatcaa atcggctatg ctgctggatt taggagtgtt ggtttcggat
      301 atcatttaca tttttgttgc acttaaattc ttctctcgtc gcgacgaaat cattgcccat
      361 gagcaggcca taactattgc cacaggggtg ttattaggct gctttggcgc ataccagctt
      421 cttaccaaaa aagcaccaag caccgatgca cccgagccca aagaaatcat tcatacccgt
      481 atcaaatccg ttggattgtt tctaaaaggc tttcttatca atatcattaa ccctaccatt
      541 ttgctgtatt ggttcggaat gatcttcatt ggtttctcca aaaacaattt cgacgattcg
      601 caaatgatcg tttttctggg atccattctg gcggctttct tttccattga tgttctgaaa
      661 atcattggtg cccgccagct taaaaaagcc atcaccccgg cgctgatgcg tcacctgaac
      721 agagcaattg gggtaattct ggttgttttt ggtgcagtac tcttcatcaa gggactccac
      781 atttttcatt gagcgtaacc gaaacaactt gtttccgtta taccgcataa ccacaatcct
      841 tctgttaaaa tcttctcaag gcgactcaca ttccaaggtt cgattttgag aacttgcatg
      901 ggatttgtaa taaaccatca aaacaatatc atgcgcttaa caacaattct ctttttactg
      961 gtcgtgttat cctctccggt tgttggtcag gaagcaacaa cgcccgttaa aaaaaccaat
     1021 gaggtagaac gcacctattg ggataaacaa aaaaagaaag tccgcagcga aggccgctac
     1081 cgtaccgacg gattttcggg tatcggactt gaagaaggag aatggaaata ctggtacgag
     1141 aacggacaaa tggaaggaat cattgagttc agtaaagggc aactcaacgg ccgtttgatc
     1201 aagtattacg aaaacggaaa aaaaatgcag gaaggttggt tcaaaatggg agtgcaggac
     1261 agcctctctt ccttctggta caacaacggt cagctgcgcg aaagcggttc atacataatg
     1321 gggaaaaaaa acggggaatg gaaatactac aaccacaccg gtaaaatcat tctcgaagaa
     1381 cggcatgaga acggtaagga attcgtggtg aactattggg atcgcaacgg aaaacaaacc
     1441 attacaagcg gtaacggaaa aatgtttgtg tatgccagcg ataatttggt gaaagaagaa
     1501 tacacctata aagacggatt gcgacacgga gcctgctatg aaaaaaatgc gcttggtcgt
     1561 gtaatcgttc agggcgaata caatctaggc aaaaaggcag ggacctggat ttttcggtat
     1621 gcagacggca ccaagaaaag agaagtttcc tataaagatg attggatgga aggcccattt
     1681 aaagaatata tgggcggtga ttccatattg gttcagggca cctataaagc cggtaaaaaa
     1741 gacggaattt gggaatggta tttcgaaaac ggaacgctcg atatgaaagg cggttttgcg
     1801 gatgatttgc aggaaggtga atggtatttc ggatacaata atggcaagat caactataaa
     1861 ggtagtttta ctgcaggcaa gcgcgaaggc aaatgggaat actactaccc cgaaggtcag
     1921 gcctacatga caggacactt taaaaacgat gtgaagcacg gcccttggat cacatggttt
     1981 gaaagcggaa aaaaactcag tgaaggcgag tttagcgaag ggaaagaaca gggcgtatgg
     2041 acctcctggt acgacagtgg tggaacaaag gacgaaggat cattcgaaaa aggactcatg
     2101 aacggcgtat ggaaaggctg gtttcccaac ggaaaactga actatcaggg tagctacacc
     2161 atgggccatc gctcagggca ctggacctat tatttttcga acggcaagaa acgtgatgaa
     2221 ggtgatttca aagtaatcag ccgctccaaa agcctcgaaa aaaacagtcc gaacgcatct
     2281 agctatcagg aaagtgttcg tcacggcaaa tgggcatcat tcgatgagcg tggagaaaaa
     2341 acagcagagg gcagctatta cgaaggagaa atggatggtc gctggttgtt ttatcaaccc
     2401 ggggaaatta tgcttgctca ggagatcaac tataaaaaag gcgtgttgga tggcgtctcg
     2461 atcagctacg acaaaaaggg caaaaagatc agcgaaatca attacaaaga cggtgcccca
     2521 catggagagt acaaaacctg ggactctaaa ggcaacctca gcaaacacct gatttacaag
     2581 aacgggaaag tggtaaagga caagctacca aagaaataag cgcctataat ttgcatctgg
     2641 tgcattttca tcgtatattt gcatcacttt cttggaaaaa cgatcagagg ggacagcggt
     2701 cccctctttt atttccctga tttttggccc ttggaagtgg tatttagaaa tgatcaacga
     2761 aagcaatatc cgtccgattg taaatgatgt ccttcaggga accgagtgtt ttctggtgga
     2821 gctggtggta gcacccggag caaagattct tgttgagttg gacagtccct ccggcgttac
     2881 gattgaacag ctccgcaaga tcagcagagc aattgaaaat cagctggatc gggaaaaaga
     2941 agatttcgag ttgacggtga gttcccccgg attggagaaa cctttcaggg tgtttgagca
     3001 atacctcaaa aacgtgggaa gaagcctgac cgttgacctc aacgacgggc ggaagctgga
     3061 aggtaaactg gaggaagcta cagaaaaagg aattgttctg gagagtagca gccgggagaa
     3121 agtggaaggc aagaaatcaa aacaagacgt agtgcgcagg catgaagtcg gaatgaatga
     3181 aattaaacag gccaaagtgc tgataacctt taaatgaccc cataaaaaca accactatga
     3241 atggtttgaa tctgatcgaa tccttccagg atttcaaaga gttcaagaac atcgacaggg
     3301 tgactatgat gagcatcctt gaggacgtat ttcgcggaat gctgaaaaag aaatacggaa
     3361 ccgatgagca catcgacatc atcctgaacg ttgaaaaggg cgaccttgaa atctggatga
     3421 accgcgagat tgtgcgcgat gaagaacttg aagacgaagg cacgcagatc gcttatagcg
     3481 aagctgtgaa aattgagccc gactttgaaa ttggtgaaga ggtatcggaa aaagttgaga
     3541 tcgacgattt tggccgccgt aatattcttg cgctgcgtca gaatcttata tcacgtgtac
     3601 ttgaacttga gaaagacaac gtatacaaaa agtacaaaga gcgcattggt gaaatcatga
     3661 ccggtgaagt gtatcagatc tggaaaaaag aaattcttat tctcgacgac gaaggcaacg
     3721 aacttattct tcccaagagc gaacagattc caagcgactt cttcaaaaaa ggagattcga
     3781 ttcgtgcagt ggtatacaaa gtggatcttc gcaacggaac tccagttgtt attctttcac
     3841 gtacttcacc tgtgttcctt gagcgtttgt ttgagcttga agttccggag gtatttgacg
     3901 gtctcatcac catcaaaaaa atcgttcgcg aacccggaga gcgtgcaaaa gttgccgtgg
     3961 aaagctacga cgaccgcatc gatccggttg gtgcctgcgt ggggatgaaa ggttcacgta
     4021 ttcacggaat cgttcgtgag ttgcgcaacg aaaacatcga cgtaatcaat tacaccaaca
     4081 ataaccagtt gcttattacc cgtgcacttt ctcctgcaaa aattacttcg gtaagtctgg
     4141 atgaagaaac aaaacgtgca gaggtttatc tgaagcccga tcaggtgtcg ctcgccattg
     4201 gtaaaggcgg tcacaacatt aaactggcca gcaaacttac aggttacgaa atcgacgttt
     4261 accgcgaagg cgcggaagat atcgacgacg ttgatctgga tgaattcact gacgaaatcg
     4321 acgcatggat ccttgacgag ctcaagtcga tcggttgcga tactgcgcgt tctgtccttg
     4381 acctaagtgt agaagatctg gtaaaacgta ccgatctcga agaagaaacc atcaacgaag
     4441 ttgttcgcat tctgaaatca gaattcgaat gaacccgctg aacaacagaa tatcgaatac
     4501 aataataaaa cccgggaaac caccgcatgt cagaatccaa agccatacgc ctgacgaaag
     4561 tcgcacgaga attaaacgtg ggactggcga ccattgtaga at
//
LOCUS       DAOMYG010000267         4594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6573_length_4594_cov_10.369024, whole genome shotgun sequence.
ACCESSION   DAOMYG010000267 DAOMYG010000000
VERSION     DAOMYG010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6573_length_4594_cov_10.369024"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(713..1060)
                     /locus_tag="PK610_09150"
     CDS             complement(713..1060)
                     /locus_tag="PK610_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRJ36143.1"
                     /translation="MKSYNPLQEKSLDFAVLVVNASMLLKERRAYELSSQFVKSGTSI
                     GANIWEAQGAESRRDFVHKLKISSKESFETVFWIKVIQRVYPDIQLDELVGLLDEIQR
                     ILSAAIKTSKNRM"
     gene            1483..2361
                     /locus_tag="PK610_09155"
     CDS             1483..2361
                     /locus_tag="PK610_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRJ36144.1"
                     /translation="MKTSGFTFIRNAIIYDYPIVEAIQSILPLCDEVVVAVGKSEDDT
                     LGLIKGIASEKIRIVETVWDDSLREGGRVLAEETNKAYAAIAKDADWAIYIQGDEVLH
                     EAYIENIRAAMLRFKDDEKVDGLLLNYLHFYGSYDYVGSSPNWYRKEIRVVRNRSSIY
                     SYRDAQGFRKGEDEKLNVIPVDAWMYHYGWVKDPRAMQKKQEEFNKLWHDDAWVKQHV
                     VKADAFDYGAHVRELRRFTGKHPVIMQQRIAAKNWTFDHDISMNKKTMKDRFKQFVLD
                     QFGWDISYKNYREIRG"
     gene            2602..3705
                     /locus_tag="PK610_09160"
     CDS             2602..3705
                     /locus_tag="PK610_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRJ36145.1"
                     /translation="MDKMCITIRPMRSGGENFMGLFFPFSEAINVELRRLHCRWSRSN
                     RCWVLPHSNSNRAILVRELESIAYLKWEGFAEENKRSLFALPRLEDHQEQQILKFTEA
                     LRAERYAANTIATYTDALRVFLRFFSDKDPSDITEDDVQRFNVDYILGNGYSASYQNQ
                     VINAIKLFFVRIEKRRIDPEILLRPRRAKPLPNVLSKEEVKRILSAPVNIKHRAMLSL
                     IYSCGLRRSELLHLKKSDIDFNRMLIRLRFAKGKKDRLVPLSEKIIRILNDYSTAYPT
                     EKYLFEGQKGGQYSEESLAKVLKMAVKKAEINKPVSLHWLRHSYATHLLDNGTDLRYI
                     QELLGHNSSKTTEIYTHVSVKSIQKIRSPFDDL"
     gene            3859..4383
                     /locus_tag="PK610_09165"
     CDS             3859..4383
                     /locus_tag="PK610_09165"
                     /inference="COORDINATES: protein motif:HMM:NF025638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HRJ36146.1"
                     /translation="MTDTVIMEELSKSYLETIANRAGYFNSVSRDYGTDLTIRKANLC
                     PTRKRYLTSGKAIDIQIKAVSERYVRHYDDNTKTFIKYDLEVKNYNDLIERAAENGAI
                     IPLILAVFIMPENKNEWVELLPEQLILKKCAFWYKIPNDRTLSNNESTVTIEIPKTNK
                     ISLDFYREQFSILD"
     gene            4383..>4594
                     /locus_tag="PK610_09170"
     CDS             4383..>4594
                     /locus_tag="PK610_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36147.1"
                     /translation="MEKYNETYIKNYFLSRNWKISREGNLFFYLKPPNELDFPQDFLL
                     EIPKENGNKESFDNYIRRLISDLSEM"
BASE COUNT         1448 a          953 c          954 g         1239 t
ORIGIN      
        1 ggcagaacaa cagaaatcgg aactcgaaca tgcgctttcc aattggaaat ccggcttgga
       61 acagaatgat gatattcttg ttattgggat tagagtgtag aatgtgcgaa tgctggaatg
      121 tgctaatgtg cgaataacct ctgctcgcga tgagattcgt gtcttaacca agttcaatag
      181 atcgttcaaa tgcggaaatg tgctagtatt cgaatgaaat agtcctcctt cacttcgttc
      241 cggaggccaa atgtgcgaat ggatactgct cgcgatgaga ttcgtgtctg aatcaagttg
      301 aaagaattgt tcaaatgtgg aaatgtgcta gtactcgaat gaaatagtcc tacttcactt
      361 cgttccggag gacaaatgtg cgaatggata ctgctcgcga tgagattcgt gtctgaatca
      421 agttcaaaga atcgttcaaa tgtggaaatg tgctagtact cgaatgaaat agtcctcctt
      481 cacttcgttc cggaggacaa atgtgcgaat ggatactgcc tgcgatgaga ttcgtgtctt
      541 aatcaagttc aaaaatcttt cacttttgaa accccatctt ctcgcgagca caatccattc
      601 gcacattgtt cgactgaagg gagaacaatc ctgaccttta gcgtcaggat tagcacactt
      661 gtccttcgca gctttagcgt agatggatta aaacactcgc acattccgta tgctacatcc
      721 tgtttttcga agtcttaatc gccgcagaaa gaatcctctg aatctcatcc agcaagccca
      781 ccaactcatc caactgaata tccggataaa cccgctgaat gactttgatc cagaaaaccg
      841 tttcaaacga ttctttcgaa gagatcttaa gcttgtgtac aaaatcacgc ctactctcag
      901 ccccctgtgc ctcccagata tttgcgccaa tcgaagtacc gcttttcaca aattgagaac
      961 tcaattcata cgcacgcctc tccttcagca acatcgaagc attcaccacc aaaacagcaa
     1021 aatcaagact tttctcttgc aaaggattat acgatttcat atcacagaaa ttaaaagccc
     1081 caaaccaaca caacccaatc caaagcaccc aaccaatttt ccccccagat tacagttcgg
     1141 tcataaatcc ctaaattgca accctttaaa tccccaaaaa aaatcattcc aacccccata
     1201 ccccattcgt tcattccaaa aatctccgat cgaatacaaa tctctccatg aagcacaatc
     1261 cattcccaca ttgttcggcc aaagggagaa caatcctgac ctttagcgtc aggattaaaa
     1321 cattagcaca ctcgcacact tgtccttcgc agctcttgcg cagaagaatt aaatcattct
     1381 cacgttacta aaaaatccta ttgaaaaccc atctcctcgc ataccacaat ccattcgcat
     1441 attcgcacat taaaccattg gcacattcat tacctttacc caatgaaaac ctccggcttc
     1501 accttcatcc gcaacgccat catctacgac tatcccatcg tagaagccat ccagtccatt
     1561 ctaccactat gcgatgaagt agtagtagca gtaggaaaat cagaagacga tacactcgga
     1621 ctgatcaagg gaatcgcatc agagaaaata cggattgtag aaaccgtgtg ggacgattca
     1681 ctgcgcgagg gaggacgcgt actggcggag gagacgaaca aggcctatgc cgctattgca
     1741 aaggatgccg attgggcgat ctatatacag ggagatgaag tattgcacga agcctatatc
     1801 gaaaatatac gagctgccat gctgcggttt aaggacgatg aaaaggtgga cggtttattg
     1861 ctgaattacc tccattttta tggttcttat gattatgtag gcagttctcc caattggtac
     1921 cgcaaggaaa ttcgtgtagt acgcaaccgg agttccattt attcctaccg cgatgcacaa
     1981 ggtttccgca agggagagga tgagaaatta aatgtgattc ctgttgatgc ctggatgtac
     2041 cattacggtt gggtgaaaga tccgcgggcg atgcagaaga aacaagagga attcaacaag
     2101 ctctggcacg atgatgcctg ggtgaaacag catgtggtta aagccgatgc cttcgactac
     2161 ggcgctcatg tgcgcgaatt gcgccgcttt acgggtaaac atcctgtaat tatgcagcaa
     2221 cgtatcgctg cgaaaaactg gacattcgat cacgatattt caatgaacaa aaaaacgatg
     2281 aaggaccgtt ttaaacagtt tgtgctggat cagtttggtt gggatattag ttataagaat
     2341 tatcgggaga taagggggtg aacggtgatc ccgacgcttt cggtcgggac aggccagtga
     2401 acggtgaaaa gtgaatggtg aaaagtgaat agtactccct tgttggatat tctcccggat
     2461 gtttttatcc tctttgattt cacagatgga tggaagtagg gatggtcttt cgttctccgt
     2521 ttccgttctc gtttcaccga accgtaatct tcctatgaat tttggtctgc acatgatttt
     2581 tggatttggt agtttgcgcg catggacaag atgtgtatta cgatcaggcc catgcgcagt
     2641 ggcggagaga attttatggg attgtttttc ccctttagcg aagcaataaa tgtagaattg
     2701 cgacgattgc attgccgatg gagccggagc aatcggtgtt gggtattgcc tcattccaat
     2761 agcaacagag cgattctagt gcgcgaattg gagtcgatcg cctatttgaa gtgggaaggt
     2821 ttcgctgaag aaaataagcg atctctattt gccttaccgc gactggaaga tcatcaggag
     2881 caacaaatcc tgaaatttac cgaggctttg cgagcagagc gctatgctgc caataccatt
     2941 gccacgtaca ccgatgcatt gcgtgtcttt ttacgctttt tttccgataa ggatccttcc
     3001 gacatcactg aggacgatgt gcagcggttt aatgtagatt atatactcgg aaatgggtat
     3061 tctgcttcgt accagaacca ggtgatcaat gcgatcaagt tgttttttgt acgcatcgaa
     3121 aagcgccgga ttgatcctga aattttgctc cgcccgcgac gcgccaagcc attgccgaat
     3181 gtattgagta aggaggaggt gaaaaggata ttgtctgcac ctgttaacat caagcatcga
     3241 gcgatgttga gtttaattta ttcttgtgga ttacgtcgca gcgaattgct tcatctgaag
     3301 aaatctgata ttgactttaa caggatgcta atccgtttac gctttgcaaa ggggaaaaag
     3361 gatagattag tgccactttc cgaaaagata attcgaatac ttaatgatta ttcaacagcc
     3421 taccctacag aaaagtacct gtttgagggg caaaagggag ggcaatacag cgaagagagc
     3481 ctggcgaagg ttcttaaaat ggccgttaaa aaggcagaaa taaataagcc ggtaagtctt
     3541 cattggcttc gccatagtta tgccacacat ttactggata atggtacaga tttgaggtat
     3601 attcaggaac tcttaggcca caatagcagt aaaaccacag aaatctatac acatgtgagt
     3661 gttaaatcca ttcaaaaaat caggagtcca tttgatgatt tatagaattt ttgataaatt
     3721 ggataatcca attaaaagta agcatagcac gccggattag cgagccatgc ttaataaagg
     3781 ttgggttata aataagttgg cagcaattat aaacgacacc gcaataagac aatttaacaa
     3841 tacagaagac agtaaagtat gacagacact gtaataatgg aagaactttc aaaaagctat
     3901 ttggaaacta ttgcaaaccg tgcaggttat ttcaatagcg tttcaagaga ctatggaact
     3961 gacctaacca ttagaaaagc taatctatgc ccgacacgta aaagatattt aacttcagga
     4021 aaagccattg acattcaaat taaagctgta tctgaaagat atgtaagaca ctatgatgac
     4081 aatactaaga catttataaa atatgactta gaagttaaaa attacaatga cctaatagaa
     4141 agagctgcgg aaaatggtgc tataattcca ctaattcttg ccgtttttat aatgccagaa
     4201 aataaaaatg agtgggtaga acttttgcct gaacagttaa ttttaaaaaa atgtgctttt
     4261 tggtataaaa taccaaatga tagaacttta tcaaataatg aaagcactgt aactatagaa
     4321 attcctaaaa caaataaaat ttctttggac ttttacagag aacaattttc aattcttgac
     4381 taatggaaaa atacaacgaa acatatatca agaattactt tctttcacgc aattggaaaa
     4441 tcagtcgtga aggaaacttg tttttttact taaaaccacc aaatgaatta gactttcccc
     4501 aagatttcct tctagagatt cccaaagaaa acggaaacaa agaaagtttt gacaactaca
     4561 ttagacgact tattagtgat ttaagtgaaa tgtt
//
LOCUS       DAOMYG010000268         4593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6578_length_4593_cov_7.557514, whole genome shotgun sequence.
ACCESSION   DAOMYG010000268 DAOMYG010000000
VERSION     DAOMYG010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6578_length_4593_cov_7.557514"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /gene="bioA"
                     /locus_tag="PK610_09175"
     CDS             <1..1033
                     /gene="bioA"
                     /locus_tag="PK610_09175"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HRJ36148.1"
                     /translation="AVVVAEKLLPLLPGNPSRLFFSDNGSTAVEVALKMAIQYFHNKG
                     KARTRIIAFENAYHGDTFGAMAVGGRSVFNTAFESLFFEVDHIPVPVQGREQECLEVL
                     DSLLRSQEPAAIILEPLVQGSAGMVMYAPEMLNEIVKRCREKGVLIIADEVFTGMGRT
                     GKLFAFDHIAEKPDIICVSKGITGGFLPLGLTACKAFIYDAFYSDDKMKALYHGHSYT
                     ANPLACAAAIASLELFEHPATQASIERITQKHKAFVKQYSGSPNFRAVRSTGTILAIE
                     FETGNSTSYFNDLRDKLYAYFIKKGLLMRPLGNVVYVLPPYCVTDDELEIAYQAIAGL
                     SHQPLTSLL"
     gene            complement(1009..1776)
                     /locus_tag="PK610_09180"
     CDS             complement(1009..1776)
                     /locus_tag="PK610_09180"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ36149.1"
                     /translation="MKRLMILLAVFLYAGSNLLKAQEYEDLAALWVDQKYEKLADKAF
                     KYTEKDKSKNDPLPYLYLAKALYRISLDEKLRAKEEFKKSEAEALSYSVKYKKKDKNG
                     IYKVDADKFFAEMKKTYFEQTENYYESGDFKKALAILKKVVQFDPENPGAWIVKAICE
                     FEMKNKTEAVNNANAGVDFVKKIQNFNDLLEPDQDFMRFALMAYANYLVKTKDVPNAK
                     KIISLGYQYYSKTKDDNGEAIKGNEDYVALYNKLVNG"
     gene            1907..2491
                     /locus_tag="PK610_09185"
     CDS             1907..2491
                     /locus_tag="PK610_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36150.1"
                     /translation="MASIKQISERISYKQHEGFSTVVISPRLDDRKQLLLTFWVFAWT
                     FSGLLFIAQLFLDYPVEMKRMIVAFLVFWLYYEYRIGYVWLWRRKGVELLKIEEGRLI
                     YKRSVRTYGKAYEYYIDNIKEFGLAEIKPGFSNVLADSFWVIGGERLRFLYQDREVRF
                     GVQLSEEEAEKLRKFLNEKFVEELKRLKEKSSEN"
     gene            complement(2509..2940)
                     /locus_tag="PK610_09190"
     CDS             complement(2509..2940)
                     /locus_tag="PK610_09190"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ36151.1"
                     /translation="MIRIIQPQTESEIENYFLLRWQTLRAPWNQPRGTERDDKENECI
                     HFLALENETPMGVCRLQYNDATNAQVRFMGVAEAGRGKGIGKLLLLEAEKIAKESGHQ
                     KMILQARENAVPFYERCGYTITEKTFLLWETIQHYLMEKKL"
     gene            complement(2937..4001)
                     /locus_tag="PK610_09195"
     CDS             complement(2937..4001)
                     /locus_tag="PK610_09195"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRJ36152.1"
                     /translation="MKDLQIVELASVLAGPSVGMFFAELGATVIKIENKKTGGDVTRT
                     WRLPSESEEGVSAYFSSVNWGKQHLFLDLNDPQDRAIALAQIAKADIVISNYKEGDDI
                     KLGMDYASLEKLNNKLIYGRISGFGPGDERPAFDVVLQAESGFMAMNGTPDSGPLKMP
                     VALIDVLAAHQLKEALLLALIKRDKKGEGSYVNVSLYDSAVCSLMNQATNYLMAHHIP
                     GRQGSLHPNIAPYGETFITNDKRFVVLAVGNDAQFKLLCNTLGDPMMPTLERYKTNPE
                     RVKNRKMLYDELAPLFYQYDRDSLMDLLIKNGIPAGAVRSMDEVFSDHRSQSLLLGEE
                     VDGQMTIRVRSTAFDPNTVL"
     gene            4165..>4593
                     /locus_tag="PK610_09200"
     CDS             4165..>4593
                     /locus_tag="PK610_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36153.1"
                     /translation="MQPSTPRKLISVLVGLVLVHPILGQIPYSAPFTAGLNGWTINTG
                     GCTAARVLTYSCGTLTTSASTNAPTCTTWNTYQNGCTSTATSPVLDFSSCSVFTTTTI
                     QVSFSIFGKIENTWDYLYVDRSIDGGATWINIGNLTGPAGA"
BASE COUNT         1181 a         1012 c         1024 g         1376 t
ORIGIN      
        1 tgcagttgtg gttgcagaaa aattacttcc cttattgccc ggaaatcctt cgcgtttgtt
       61 tttttcagac aatggttcca ctgcggttga ggtagcactc aaaatggcca ttcagtattt
      121 tcacaataag ggaaaagcaa gaacacgcat cattgcattt gagaatgcct accacggcga
      181 tacattcggt gcaatggctg ttggtggtcg ttctgttttt aatactgcat ttgaatcgct
      241 tttttttgaa gtcgatcaca ttcccgttcc tgtgcaggga agagaacaag aatgtttgga
      301 ggtgttggat tcacttttgc gaagtcagga accggcagcc attattcttg aaccactagt
      361 acaaggaagt gcaggtatgg tgatgtatgc gcctgaaatg ctgaatgaga tcgtgaaacg
      421 ctgccgtgaa aaaggtgtgc tgattatagc tgacgaagta tttaccggga tgggtcgcac
      481 cgggaaatta ttcgccttcg atcacattgc agaaaagccg gatatcattt gcgtttcaaa
      541 gggaataaca ggtggatttt tacccttggg gttgacagcc tgcaaagcat ttatttacga
      601 tgcgttttat tcggacgata aaatgaaagc attgtatcac ggacattcct acacggccaa
      661 tccgctggcc tgcgctgctg caattgcttc actcgaattg tttgagcatc ctgcaaccca
      721 ggcatccatc gaacgcatta cgcaaaagca taaagcattt gtaaaacagt attcgggttc
      781 gcccaatttc agagctgttc gttcaaccgg aaccattctg gccattgaat ttgaaaccgg
      841 gaattccact tcctatttca atgatctgcg tgataaattg tacgcttact ttataaaaaa
      901 aggcctcttg atgaggcctt tggggaatgt ggtgtatgtg ctacctccgt actgtgtaac
      961 ggatgatgaa ctggaaatcg cttatcaggc tatagccgga ttaagccatc aaccgttgac
     1021 gagtttgttg taaagcgcaa cgtaatcttc atttccttta atggcttcgc cgttgtcatc
     1081 ttttgttttg gaataatact ggtaccccag tgaaataatt tttttcgcgt tgggaacatc
     1141 tttcgttttt accaggtagt tggcgtaggc cattaaagcg aagcgcataa agtcctgatc
     1201 aggctccaga agatcgttga aattctgaat cttcttaacg aaatcaactc cggcattggc
     1261 attgtttacc gcttcggttt tgtttttcat ttcgaactcg cagattgctt tcacaatcca
     1321 tgcacccggg ttttcaggat cgaattgtac taccttttta agaatagcca acgctttttt
     1381 aaagtcgccc gactcataat agttttcggt ttgctcgaaa tacgtttttt tcatttcagc
     1441 aaaaaacttg tcggcatcca ctttatagat tccgtttttg tcttttttct tgtacttcac
     1501 cgaatagctc agcgcttcag cttccgattt tttgaattct tccttagcac ggagtttctc
     1561 gtcgagcgaa atgcggtaaa gtgctttcgc caggtagagg tagggcaggg gatcgttttt
     1621 ggatttgtcc ttttcagtgt acttaaaagc cttatcggcc agtttttcgt acttctggtc
     1681 aacccaaagc gctgcgaggt cttcgtattc ctgggctttc agcaggttag agccggcata
     1741 aaggaacacg gcaagaagga tcatcaggcg tttcataatg gtaatggttt tggtttgatt
     1801 gagacaaaac aaatataatg cctgaaaacg aaaagccgcg ataatattga aggaataaat
     1861 cacacccgct tgttagcaca gtgggggatt tgggtatttt tgccgcatgg cctccataaa
     1921 acagatctca gaacgtatca gttacaaaca acacgagggc ttttcgacag tagtcatctc
     1981 accacgactt gatgaccgta aacagctgtt gctcactttt tgggtgtttg catggacatt
     2041 ctccggactc ctctttatcg cgcaattgtt cctcgattac cctgtggaaa tgaagcggat
     2101 gattgtcgcg tttctggttt tctggctgta ttacgagtac cggatcggat atgtgtggct
     2161 gtggagaaga aaaggagtgg agctgctgaa gattgaagaa ggacgactga tctacaaacg
     2221 ctctgttcga acctatggca aggcttacga atattatatc gataacatta aagaattcgg
     2281 attggccgaa atcaaacccg ggttttcgaa tgtgctggcc gattcgttct gggtaattgg
     2341 tggtgagcgc ctccgtttcc tgtatcagga tcgtgaggtt cgttttggtg ttcaactctc
     2401 cgaagaagaa gcagagaaat taaggaaatt cctgaatgag aaatttgtgg aagaactgaa
     2461 acgtctcaag gaaaagtcat ctgaaaatta agttcaagct tttacacttc aaagtttttt
     2521 ctccatcagg taatgttgta ttgtttccca gagtagaaat gttttttcag taatggtgta
     2581 accgcatcgc tcgtaaaacg gaactgcatt ttctcttgcc tgaagaatca ttttttggtg
     2641 accgctttct ttggcaattt tttcagcttc gagtaaaagt aattttccaa ttccttttcc
     2701 cctacctgct tctgcaactc ccataaaacg aacctgtgca tttgtggcgt cgttgtattg
     2761 caagcgacaa acgcccattg gtgtttcgtt ttcaagcgcc agaaagtgga tgcattcatt
     2821 ttctttatca tcacgctcag taccccgggg ttgattccac ggtgcgcgca aggtttgcca
     2881 gcgaagaaga aagtagtttt cgatttccga ctctgtttgt ggctgaataa tccggatcat
     2941 aatacagtgt tagggtcaaa tgcggtggag cgaacgcgaa tggtcatttg tccgtcgact
     3001 tcctctccaa gtaaaagcga ctgcgaacga tggtcggaaa aaacttcgtc catactgcga
     3061 actgcacctg caggaattcc gtttttgatc agcaagtcca tcagcgaatc gcgatcgtac
     3121 tgatagaaca aaggagcaag ttcgtcgtac agcatttttc tgtttttaac ccgttcagga
     3181 ttggttttgt accgctccag cgttggcatc atcggatcgc ccaatgtatt gcacaacaat
     3241 ttaaactgag catcattacc aactgcgagt acaacaaagc gtttgtcgtt ggtaataaac
     3301 gtttctccgt aaggagcaat attgggatgc agcgatccct gtcggcccgg aatatgatgt
     3361 gccatgaggt aattggtagc ctgattcatc agcgaacaaa ccgcagaatc gtacagcgac
     3421 acattcacat aactgccttc gccttttttg tcgcgtttta ttaaagccag taacaatgct
     3481 tctttcagtt gatgggcagc aagcacatcg atcaaggcaa caggcatttt aaggggacca
     3541 ctatccggcg ttccgttcat ggccataaag ccgctttccg cttgtaaaac cacgtcgaag
     3601 gcaggacgct catcgcccgg accaaatcct gaaattctgc cgtaaataag tttgttgttc
     3661 agtttttcca gtgatgcata atccatccct aatttaatat catcaccctc tttgtaatta
     3721 gaaatgacaa tatcagcttt tgcaatttgc gcaagtgcaa tagctcgatc ctgcggatca
     3781 ttgagatcaa ggaaaagatg ctgcttgccc cagttcacac tgctgaaata ggccgaaaca
     3841 ccttcttccg attcgctggg caaacgccag gtgcgggtga catcgccacc ggtttttttg
     3901 ttttctattt tgataacagt agcacctagt tcggcaaaga acattcccac cgagggacct
     3961 gccagcacgg aagccagttc aacaatttgc aaatctttca ttctctcaaa gatggagaat
     4021 taaaaccttt ttgctgcgat tgttcccttt gtttttattg ttgttcaaag tgttgtcgct
     4081 tgtatctcac cgttttatca agttttccac agaggcaact ttttctaccc attaatggtt
     4141 aaatttgggt aaatctgcct tgccatgcaa ccttcaacgc cccgaaagct gatttcggtg
     4201 cttgtgggac ttgttctcgt acatcctatt ttgggacaaa ttccctacag cgcacccttt
     4261 accgccggac taaacggctg gaccattaac actggtggat gtactgccgc cagagtgtta
     4321 acttattcct gtggaacgct gaccacaagc gcctctacaa acgctccaac ctgcacaaca
     4381 tggaatacct atcagaacgg atgtacatct actgcgacct ctcctgttct tgatttttca
     4441 tcctgtagtg tttttaccac aacaaccatc caggtgagtt tttccatttt cggaaaaatt
     4501 gaaaatactt gggactatct gtatgtcgac cgcagcattg atggcggagc aacatggatc
     4561 aacataggta acctgaccgg tcctgcgggt gca
//
LOCUS       DAOMYG010000269         4584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6594_length_4584_cov_7.544491, whole genome shotgun sequence.
ACCESSION   DAOMYG010000269 DAOMYG010000000
VERSION     DAOMYG010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6594_length_4584_cov_7.544491"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PK610_09205"
     CDS             complement(<1..452)
                     /locus_tag="PK610_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36154.1"
                     /translation="MRYFVFLLLLFPGSDLFAQKVIADTIFPVRSSMRYSQLVNVTED
                     PSTGNIQLIFRQTNQTNSRSFFRYTFSPELQLLENEMEDQYYSDEDHLRRMYPNYRGE
                     HFTEEGFAVQQSMRIGDRQKIEFIRYYDSYTWNWFIRDYFVQRQITGAR"
     gene            complement(478..1410)
                     /locus_tag="PK610_09210"
     CDS             complement(478..1410)
                     /locus_tag="PK610_09210"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRJ36155.1"
                     /translation="MTIQQLEYVLAIADTGSFVEAAGRCFVTQPTLSMQVKKLEDDLE
                     IKLFDRNTHPVELTEMGRDFVVRARRIVQESRALREFVREGKEELSGSLNIAVIPTLS
                     PYLLPLFAGKFLRSNPKIELTITEYTTSEIVQRLRTGQLDAGILATPLQESGIIERPV
                     FYEEFVAYVSKDHPLRKKNVLQANDIDAEEMWVLQEGHCFRNQVLNFCKVKGSGQYSR
                     LHYESGSILALKRMVDAENGLTLLPELAVRDFGKREMDQVRYFKSPAPVREISLITSR
                     FPVKERLLDVLEKSIIEAVPVKMHKKGKSKKIDI"
     gene            1499..1945
                     /locus_tag="PK610_09215"
     CDS             1499..1945
                     /locus_tag="PK610_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HRJ36156.1"
                     /translation="MSTSTLTQHLNRILAGETQLALLTRNAHWNLISFHFKAVHEWLG
                     ELYNTTNTHIDEIAERIRMIDGIANASLKTYLDESPVPDHSAPIQTEEDTLRLLQDAH
                     LKLIAAIKEGIFLADEANDPGTEDFLTGLLREHEKTLWMLKATKGS"
     gene            2065..4245
                     /gene="katG"
                     /locus_tag="PK610_09220"
     CDS             2065..4245
                     /gene="katG"
                     /locus_tag="PK610_09220"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HRJ36157.1"
                     /translation="MSQTDLSKCPFHNGQMEQTAGAGTSNKDWWPNRLNLNILRQHSE
                     LSNPYRNDFNYAEEFKKLDLKEIKKDIFNLMTTSQDWWPADYGHYGPLFIRMAWHSAG
                     TYRISDGRGGASTGNQRFAPVNSWPDNGNLDKARFLLWPIKQKYGKKISWADLMILAG
                     NCALESMGFKTLGFAGGREDIWQPEEDINWGNETTWLGDKRYHGERELENPLAAVQMG
                     LIYVNPEGPNGNPDPLASAKDVRETFARMAMNDEETVALIAGGHTFGKAHGAASADHV
                     GREPEAASIEEMGMGWRNSYGSGKGADTITSGIEGAWKPYPTKWDNGYLETLFKYDWK
                     LVKSPAGAHQWIPTDEKAVDQIEDAHDPNIRHAPMMTTADLALKFDPIYEPIARRFKE
                     NFAEFEKVFAHAWFKLTHRDMGPVSRYLGEEVPGETMIWQDPVPASESNLNDKSIAEL
                     KEMIFESGLSISQLASTAWASASTYRNSDKRGGANGARIRLEPQINWEVNNPVQLKKV
                     LATLENIKDSAEKKGIHVSLADLIVLGGCAAIEKAASNGGYNTCVPFTQGRGDASKEL
                     TDASSFNVLEPKADGFRNYKSVDCFSVTEKMLIDKAQLLTLTAPEMTALIGGMRVLNT
                     NYNGSSHGVFTSKKETLSNDFFINLLDLGVTWKATDKEGEIFEGMDRTTGNVKWTATR
                     ADLIFGSNSELRAIAEIYASVDGKEKFINDFVTAWVKVMNLDLF"
BASE COUNT         1453 a          954 c          925 g         1252 t
ORIGIN      
        1 cgtgctcctg ttatttgtcg ttgaacaaaa tagtcgcgta taaaccaatt ccaggtgtag
       61 gaatcgtaat aacggatgaa ttcaattttt tgtctgtcgc caattcgcat gctttgttga
      121 acagcaaaac cttcttctgt aaaatgctcg cccctgtaat tcggatacat tcggcgcaga
      181 tgatcttcgt cggaataata ctgatcttcc atttcattct ccagcaattg caattcagga
      241 ctaaatgtat acctgaaaaa agatctggaa ttggtttgat tggtttgacg gaatatcaat
      301 tgaatgttac ccgttgaagg atcttcggta acatttacca actgcgaata ccgcatcgaa
      361 gaacgtaccg gaaaaatagt atccgcaatg accttctgcg cgaaaagatc gctgccagga
      421 aataacaata acagaaaaac aaaatatctc ataagcaatt gtttatcaaa tatcatgcta
      481 aatatcgatt ttttttgatt tacctttctt gtgcattttc acaggcactg cttctattat
      541 gcttttctcc aaaacatcca gcaagcgctc ctttaccgga aaacggcttg tgataagtga
      601 aatttcccgg acaggtgcag gacttttaaa atacctcacc tgatccattt cccgtttgcc
      661 aaaatccctt acagccaatt cgggtagaag ggtcaaacca ttttctgcat ccaccatacg
      721 ttttagagca aggatgcttc ccgattcgta atgcaagcgc gaatactgac cactcccctt
      781 caccttacaa aaattcagca cctgattccg aaagcagtgc ccctcctgca atacccacat
      841 ttcttcagca tcaatatcat ttgcctgcaa cacattcttt tttcggagcg gatgatcttt
      901 cgaaacgtaa gcaacaaact cttcataaaa aacaggacgc tcaatgatgc ccgattcctg
      961 caaaggggtt gcgagaatgc ctgcatccaa ttgtcctgtg cgcaaacgct gaacgatttc
     1021 ggaggtggtg tattcggtaa tggtcaactc aattttcgga ttgctgcgaa ggaattttcc
     1081 cgcgaacaaa ggcagcaaat aaggcgacaa ggttggaatt acggcaatgt ttaatgagcc
     1141 gctaagctct tcttttccct cacgaacaaa ctcacgcaat gcacgactct cctgcacaat
     1201 cctgcgggca cgaacaacaa aatcacgtcc catttcagtc aattcaacag gatgcgtgtt
     1261 cctgtcaaac aacttgattt caagatcgtc ctccagcttt ttaacctgca tgctgagtgt
     1321 cggctgggta acaaaacacc gtcctgcagc ttctacaaaa cttccggtat cggctatcgc
     1381 caacacgtat tccaattgtt gaatagtcat atcaataaaa tctataagtc tataaaatta
     1441 atcaatttta tttatttaat aaatgccctg acctttgatc cggataaaat aaaccactat
     1501 gagcacttca accctgaccc aacatctcaa ccgcatactg gctggtgaga ctcaattagc
     1561 attacttacc cgtaatgccc attggaacct gatttcattc cactttaaag ccgtacatga
     1621 atggcttgga gaattgtaca acacaaccaa tacacatatc gatgagattg cggaacggat
     1681 ccgcatgatt gatggtattg cgaatgcttc cttaaaaacc tacctcgatg aaagtcctgt
     1741 accggaccat tcagccccta ttcaaacaga ggaagacact ctacgccttt tgcaggatgc
     1801 gcatttgaaa ttgattgcag ccattaagga aggaatattc cttgcagatg aagcgaacga
     1861 tccgggaaca gaagatttcc taaccggcct ccttcgcgaa catgagaaaa ccttatggat
     1921 gttgaaagca acgaaaggaa gctaaccact gaataggttc agatgaaata atgttctatt
     1981 cttcaaatag taacttacat ccttttttac ctcaaaacat cttcctaaat tatacttggt
     2041 attcaacact taaaaacttc taacatgagt caaacagatt tgagtaaatg tccattccat
     2101 aatggtcaaa tggaacaaac ggctggtgca ggaacctcca ataaagactg gtggccaaat
     2161 cgtttaaatc tgaatatact tcgtcagcac tctgaattat caaatccgta tcgaaatgat
     2221 ttcaactatg cagaggaatt caaaaaactg gatttgaaag aaattaaaaa agacattttt
     2281 aatttgatga caacttcaca ggattggtgg ccggctgatt acggacacta tggacctctt
     2341 tttatccgta tggcctggca cagtgcagga acgtatcgta tttcagatgg ccgaggcgga
     2401 gccagtacag gaaatcagcg attcgctccg gtaaacagct ggcctgataa tggaaatctt
     2461 gataaagcac gatttctatt gtggcctatc aaacagaaat acggaaaaaa aatctcctgg
     2521 gcagatttaa tgatacttgc aggtaattgc gctttggaat ctatgggatt caaaacattg
     2581 ggatttgcag gtggacggga agatatttgg caaccggaag aggatatcaa ttggggaaat
     2641 gaaacaacgt ggcttggtga caaacgctat catggagaac gtgaacttga aaaccctctt
     2701 gcagcagtac aaatggggct tatttatgta aatcccgaag gtccaaacgg aaatcctgat
     2761 cctcttgcat cagcaaaaga tgtgcgtgag actttcgcgc gaatggccat gaatgatgaa
     2821 gaaacagttg cactcatagc aggtggacat accttcggaa aagctcatgg agcagctagt
     2881 gctgatcatg ttggtcgtga gcctgaagct gcatccatag aagaaatggg aatgggatgg
     2941 agaaatagct atggatcagg taaaggagct gatacgatta ccagtggaat tgaaggtgca
     3001 tggaaaccct accccacaaa atgggacaat ggttacttgg aaacattatt caagtacgat
     3061 tggaaacttg taaaaagtcc ggcaggtgca caccaatgga ttccaactga cgaaaaagcg
     3121 gttgaccaga ttgaagatgc acatgatcca aatattcgcc acgcgccaat gatgacaact
     3181 gcagatcttg cactaaaatt tgatccaatt tacgaaccca ttgcgcgtcg tttcaaggaa
     3241 aattttgcag aatttgaaaa agttttcgct catgcatggt tcaaattaac ccacagggat
     3301 atggggccag tttcacgcta ccttggtgaa gaagtaccag gagaaacaat gatctggcaa
     3361 gatcctgttc ccgcatctga gagcaatcta aatgataaat caattgcaga actcaaagaa
     3421 atgatatttg aaagcggact cagtatttca caactggcaa gcactgcttg ggcttctgca
     3481 tcaacctacc gtaattctga caagcgtggg ggagcaaacg gagcgcgcat tcgcctcgaa
     3541 cctcaaatta attgggaagt aaataatcct gtgcaattga aaaaagtact tgccacactg
     3601 gaaaacatta aggattcagc cgaaaaaaaa gggattcatg tttcacttgc tgaccttata
     3661 gtcctgggtg gttgcgcagc gattgaaaaa gcagcatcaa atggtggtta caatacatgc
     3721 gtgcctttta cgcaaggaag aggcgacgca agtaaagaac taacagatgc atcatccttt
     3781 aatgtgcttg aacctaaagc agatggtttc agaaattaca aaagtgttga ctgcttttca
     3841 gtcacagaaa aaatgcttat cgacaaggca cagctactca cgcttactgc acccgaaatg
     3901 acagctttga ttggcggtat gcgtgtatta aataccaatt acaatggttc gtcacacggt
     3961 gtttttactt cgaaaaaaga aacgctcagt aatgacttct tcatcaatct gcttgatctt
     4021 ggtgttacat ggaaagcaac tgataaagaa ggtgaaattt tcgaaggaat ggatcgtaca
     4081 accggaaatg ttaaatggac tgctacaaga gcagacctta ttttcggatc gaattcagaa
     4141 ttaagggcta tcgcagaaat ctatgcaagc gttgatggaa aagaaaaatt cataaacgat
     4201 tttgttactg cctgggttaa agtgatgaac ctggatttat tctaattttt ccaaataaaa
     4261 gttgaaccgt gaatcaagtt gattcacggt tcattttcta tctctatttt ttttataact
     4321 gcataaataa ttccctgaaa ttagagcggt caatgattcg ctgattagct taatatgccg
     4381 aaagaaatgt aggaggtatc taattatatt ttctgtttgt ccatttgaat tcttaagcca
     4441 ttagttgact tgcatcttct ttcatcaatc tcataaacat gacttttggt gaacataatc
     4501 accgtaggtt atggcttttg gtgaattgga aatcgcggaa aaatacaaat aattgatttt
     4561 catttattta aataataatc agct
//
LOCUS       DAOMYG010000270         4582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6597_length_4582_cov_9.768942, whole genome shotgun sequence.
ACCESSION   DAOMYG010000270 DAOMYG010000000
VERSION     DAOMYG010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6597_length_4582_cov_9.768942"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="PK610_09225"
     CDS             complement(<1..170)
                     /locus_tag="PK610_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRJ36158.1"
                     /translation="MNTTLTGTVKFFNSEKGFGFIKHDDSDTETFVHKTGLRTDVQQN
                     DRVRFTIEEGRKG"
     gene            complement(307..2055)
                     /locus_tag="PK610_09230"
     CDS             complement(307..2055)
                     /locus_tag="PK610_09230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRJ36159.1"
                     /translation="MLSILTKASFDPFSQPLIILLQPFSCLIVGLSKLHLIMYEQDPK
                     HLLPEPLPQPDARRSLVYVPFLLAIALAAGVYFGSRMVVIPKGSQKETVSRLNKLINI
                     IEDRYVDKVDRESLTENAMRDILLGLDPHSSYMSPDDAQKAHEELSGNFGGVGIQFLV
                     FKDTLVVTHLIADGPSERAGIKKFDRIVDVDGHSMARKEITTEDVFKRLRGEMGTKVK
                     LKIKRQGEKKLISFELERDAIPVSTVETAIMVENGIGYIRLNQFGAQTHSDFLQAVHR
                     LQKKGMTSLILDLRDNGGGYLKEANLIADEFLESGQLIVYTEGEHSPKHTYKATKKGR
                     LEKTKVAVLINHNSASASEIVAGALQDNDRGIIIGRRSFGKGLVQEEISPFDDGSSIR
                     LTVARYYTPTGRSIQKPYGKGIDYEMEQIERFERNEFFVPDSSIFVDSLKFTTAKGKV
                     VYGGGGIMPDIFVPIDTTGRTNFYSNLLYQRVIAEFAFDFVERYRQVLLRYTDASMFA
                     GSFAVEEQVFRELLNYAEKKGLRRYPGEAEHSRRLIQNQIKAEIARLVWNTDGYFAVL
                     AKNDSDLLKALEVLKK"
     gene            complement(2024..2458)
                     /locus_tag="PK610_09235"
     CDS             complement(2024..2458)
                     /locus_tag="PK610_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HRJ36160.1"
                     /translation="MKDNQKQQRYDRAYLKMAMEWGKLSHCTRKQVGALIVRDNMIIS
                     DGYNGTPSGFPNACEDENGETNWYVLHAEANAILKVAKSTQSAAGATLYLTLSPCKEC
                     SKLVLQAGIRRLVYINPYKDQSGVDFLRSAGIDVVHFNESEL"
     gene            complement(2557..3510)
                     /locus_tag="PK610_09240"
     CDS             complement(2557..3510)
                     /locus_tag="PK610_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ36161.1"
                     /translation="MKKSLLAFAVLAFAGNVMAQSAAGGPDSFGYTFKDSNEPGGPGY
                     SWIELNPALGGGGTVVPNVNQDDNHEPNVALGFTFPFYGNNFTQISVATNGTVYFEDV
                     YLGLSNECIPGTPTYTMTDYRFIAHMWNDLDPSSQGNIWMQSFSTYFVLQFDNIVPCC
                     GAGDGDTWQLILYSSGNIRMQYKELSNTDNFGDATVGIQNDPTTGLQYMCDNAPNALA
                     SNLAILFVHPNAASVQEDASNLVNVFPNPTNGIITVDATAISGLFQFEVVNQLGQVVH
                     TATMNGASTSTVDLSGLSAGIYHVKLQNESMFASKKIVLQN"
     gene            complement(3622..>4582)
                     /locus_tag="PK610_09245"
     CDS             complement(3622..>4582)
                     /locus_tag="PK610_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRJ36162.1"
                     /translation="PALMRELALEILRRNLNVTWWTNIRFEKSFTKDLCRLLAASGCI
                     AVSGGLEVASDRLLELIDKGVTVEQVTKVAAHFTECGIMVHAYLMYGYPTQTMQETVD
                     SLEVVRQLFENGVLQSGFWHRFALTTHSPVGMHPEKFGIKITQQEKGTFANNDLEFVD
                     PTGCDHDAFAEGLRISLYNYMLGAGYDLPLQKWFDNKIPKSGIPRDYIKQVLDKEEFK
                     EIPASAKLIWIGGKIQLGAIPGKGNKKGRTRISIHTRNRSHQIAMDEEDAKWLIDEFA
                     TSYTVKTFGTVRSEYEKRTGKNFLLFWHQSEMDLVREAGLIIV"
BASE COUNT         1238 a         1020 c         1019 g         1305 t
ORIGIN      
        1 cctttacggc cttcctcaat ggtgaaacgg acacggtcgt tctgctgaac atcagtgcga
       61 agaccggtct tgtgtacgaa tgtttcagtg tctgaatcat cgtgcttgat aaatccgaag
      121 cctttttcgg aattgaagaa cttaacagtt cctgtcaggg tggtattcat tgtgttaggg
      181 attaagattg ctgagggatg gatccggact taggggagaa ataatggtgt tgttttaaga
      241 attgcgccgg atagtacaag cgtttaccaa agataaactt tttggataat tgtccaactc
      301 ccccaattat tttttcagaa cctccaacgc tttgagcaag tcgctatcgt ttttggccaa
      361 aaccgcaaaa taaccatcag tattccaaac gagccgggca atttctgctt tgatctggtt
      421 ttggatcagt ctgcgcgaat gctcggcttc gcccgggtag cgacggagac cttttttctc
      481 ggcataattg agcaactcgc gaaacacctg ctcttctacc gcaaatgaac ctgcaaacat
      541 ggaggcgtcg gtataacgaa gcaacacctg tcggtagcgc tcaacaaaat caaaggcgaa
      601 ttcggcaata actctttgat agagtaaatt ggaataaaag tttgttcttc cggtggtgtc
      661 aatcggcaca aaaatatccg gcatgatgcc cccgcctcca taaacgactt ttcctttggc
      721 ggttgtaaat ttcagggagt cgacgaagat ggaactatcg ggaacgaaaa actcgtttcg
      781 ctcaaaacgc tctatttgtt ccatttcgta atcaatgcct ttaccgtatg gcttttggat
      841 agacctgccc gttggagtgt aatagcgggc aaccgtgagg cgaatggaag aaccgtcatc
      901 aaatggactg atctcttcct gaacaaggcc tttaccaaag gagcgtcttc caataattat
      961 tcctctgtcg ttatcctgca acgcacccgc aacaatctcg ctcgctgagg cggaattgtg
     1021 gttgattaat accgcaactt ttgttttttc aagacgaccc tttttggttg ctttatacgt
     1081 gtgtttcgga ctgtgttcac cttcggtata aacgatgagc tgaccacttt caaggaactc
     1141 atcagcaatg aggttggctt ctttcaagta acctcctccg ttgtctctaa ggtcgagaat
     1201 gaggctggtc attccttttt tctgtaatcg atgtacagcc tgcaaaaagt cggaatgtgt
     1261 ttgtgctccg aattggttca aacggatgta tccaattcca ttttcaacca taatggcggt
     1321 ctctaccgtg ctaaccggaa tagcatcgcg ttccagctcg aaactgatca gttttttttc
     1381 gccctgacgt tttattttca gtttcacctt ggtacccatt tcacctcgca aacgcttgaa
     1441 cacatcttcg gtggtgatct ctttgcgcgc catggaatga ccgtcgacat caactatacg
     1501 atcgaatttt tttatcccgg cacgctcaga tggaccatcg gcgataaggt gcgtgactac
     1561 aagcgtgtct ttgaaaacca ggaattgaat accaacacca ccaaaatttc cgcttagttc
     1621 ttcgtgtgcc ttttgggcat catcaggact catataactt gaatgcggat ccaatccaag
     1681 cagaatatcg cgcattgcat tttcggtaag cgattcccga tccaccttat ccacgtaacg
     1741 gtcttcgatg atattgatga gcttgttcaa gcggctgacg gtttcctttt gggagccctt
     1801 tggaataacc accattcgcg atccaaaata taccccggca gcaagagcaa tagccagtaa
     1861 aaaggggaca taaactaggg atctccgcgc gtcgggctgt ggaagtggtt caggtaacag
     1921 atgtttggga tcttgctcgt acatgatgag gtgcaattta cttaatccaa cgattaagca
     1981 ggagaatggt tgaagaagaa tgatgagtgg ctgagaaaac ggatcaaagc tcgctttcgt
     2041 taaaatggac aacatcaatt ccggcactgc gcaggaaatc tactccgctt tggtctttat
     2101 agggattgat ataaacaagt cttcggatcc ccgcctgaag aacgagttta ctgcattcct
     2161 tgcatggcga aagagtcaga tagagagttg cgcctgccgc actttgtgtt gacttagcca
     2221 cctttaaaat ggcattggct tcggcgtgga gcacatacca attggtttct ccgttttcat
     2281 cttcgcaggc attcggaaat ccggaggggg tgccattata gccgtctgaa atgatcatgt
     2341 tgtcgcgtac aatcagcgcc cctacttgtt tgcgggtgca atgggatagt ttaccccatt
     2401 ccattgccat tttcaggtag gcgcggtcgt agcgttgttg tttctggttg tctttcattg
     2461 ccgggcaaaa atacaaagct gtgggtaagt actgcagaat aaaaaaagaa aaagccccgt
     2521 ttttggcggg actttcaatt atttatcaga aagaaatcaa ttctgaagaa cgattttttt
     2581 agaagcaaac atgctttcgt tctgaagctt tacgtggtaa atacctgcag aaagaccaga
     2641 aagatctaca gtagatgtag aagcaccgtt cattgttgcg gtatgtacaa cctgacctaa
     2701 ttggttcacc acttcaaatt ggaaaagtcc gctgatagca gtagcatcaa cagtgatgat
     2761 tccgttggta gggttaggga acacatttac gaggtttgac gcatcttcct gtacgcttgc
     2821 tgcattggga tgaacaaaaa gtatagcaag attcgaagcc aaagcgttgg gagcgttatc
     2881 gcacatgtac tgaagacctg ttgtaggatc gttctggata cctactgtag catcaccaaa
     2941 gttatcggtg ttgctgagtt ccttgtactg catacggatg tttccgctgc tgtaaaggat
     3001 caactgccat gtatcaccat caccggcacc gcaacaggga acgatgttat cgaactgtaa
     3061 aacaaaatag gtagagaacg actgcatcca gatattaccc tgtgagcttg ggtcaaggtc
     3121 gttccacata tgagcgataa agcggtaatc ggtcatcgtg taagttggtg ttccgggaat
     3181 acattcattg gaaagaccca gatacacatc ttcaaaatat accgttccgt tggtggcaac
     3241 actgatttgt gtgaagttgt taccgtaaaa aggaaaggtg aaacccagag caacattggg
     3301 ctcatggtta tcatcctggt ttacgtttgg aacaacagta ccaccaccac caagtgcagg
     3361 gtttaattca atccagctgt aaccgggacc accgggttcg tttgagtctt tgaaagtgta
     3421 accaaaggaa tcaggtcctc cggcagcaga ttgtgccatt acattgccgg caaaagcaag
     3481 aacagcaaaa gcaagtaaag attttttcat agaaaatgat ttgatttatt ttggacaaag
     3541 ataggaattc aagattgcct gaaaaaacga aattttcaac aaaagactga ttaacatcat
     3601 tttgcacgaa aagactattg ttcagacaat aatcaatccg gcttcgcgga ccaaatccat
     3661 ttcagattgg tgccagaaaa ggagaaagtt ttttccggtg cgtttttcat attcgctgcg
     3721 cactgtacca aaagttttca ctgtatatga agttgcaaat tcatcgatca gccattttgc
     3781 gtcttcctca tccattgcaa tttggtgcga tcgattgcgg gtgtgaatcg aaatacgtgt
     3841 acgtcctttt ttatttcctt ttccaggtat cgcaccaagt tggatttttc caccgatcca
     3901 aatcaatttg gcacttgccg gaatttcctt gaactcttct ttatcaagaa cctgtttgat
     3961 atagtctctg ggaattccgc tttttgggat tttattatca aaccatttct gcaaaggcaa
     4021 atcataacct gcgcctagca tataattgta gagcgagatc cggagtcctt cggcaaaggc
     4081 atcatgatca cagcctgtcg gatcaacaaa ctcaagatcg ttattggcaa atgttccttt
     4141 ttcctgctgc gtaattttta tcccgaattt ttcaggatgc attccaaccg gactgtgcgt
     4201 ggtaagcgcg aatcgatgcc agaaacccga ttgcaatacg ccgttttcaa acaattgcct
     4261 taccacctct aacgaatcaa cggtttcctg catggtttga gttggataac cgtacatcag
     4321 gtatgcatga accataatcc cgcattcggt aaagtgagct gcaacttttg ttacttgttc
     4381 taccgttacg cctttatcaa taagttccag caaacggtcc gatgccactt caagtcctcc
     4441 tgatactgca atgcaaccag aagcggcaag caggcgacat aaatccttcg taaaactttt
     4501 ttcaaagcgg atgtttgtcc accacgttac attcaaattt cttctgagaa tttccagcgc
     4561 aagttcacgc atcaatgcag ga
//
LOCUS       DAOMYG010000271         4578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6605_length_4578_cov_8.075171, whole genome shotgun sequence.
ACCESSION   DAOMYG010000271 DAOMYG010000000
VERSION     DAOMYG010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6605_length_4578_cov_8.075171"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PK610_09250"
     CDS             complement(<1..595)
                     /locus_tag="PK610_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36163.1"
                     /translation="MSFFSISDALLVSVWLFLMLWLIRSHSFFRIQELQPRFITYVFL
                     LKVVAGFFLTIIYTFYYTDRANADIYKYFDDSALMTDALFHKPGDFFRMLFGYENDNN
                     YFNEQYYNHMNNWFRKYESNIYNDSHTIIRINAVLRIFSFGSFHVHTLFSCFLSLTGL
                     VALYKAFKSFFTGKEKWMAYAIFLLPSVLFWASGVLKE"
     gene            complement(644..1501)
                     /locus_tag="PK610_09255"
     CDS             complement(644..1501)
                     /locus_tag="PK610_09255"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRJ36164.1"
                     /translation="MCFEIIHLHVIRPGILYMLTATAGFAVVNFLVKFLDHIPTSEIV
                     FFRSLITLVLTAGMVIHKGLPFFGNNHFWLLVRGVFGASALILFFYTLKELPMATATT
                     VQYLSPVFTVVIAMFLVGEKVKPIRWVFFVVSFAGLLMMEGYQEKPNWTFLWLGVLSA
                     FLSAVAYNAIIKCRDTDHPYTVVMAFPLIAAPITGIWAMNEWVQPQGVDWVYLLLIGL
                     FTQVAQVFMTMALHAEKAAVITPFKYLGTVYALLLGYLFFDETFTFMSLIGMFIVVIS
                     VVVNALVKS"
     gene            2054..3229
                     /locus_tag="PK610_09260"
     CDS             2054..3229
                     /locus_tag="PK610_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRJ36165.1"
                     /translation="MSSYRRLFLEHLAQTSDFPMAVEIARAEGCYMYGPDGKKYLDLI
                     AGISVSNLGHGNPAIKKAIKEQVDTNMHLMVYGEFVQASQVKLATALHKATHPTLTQT
                     YFTNSGSEAIEGAMKLAKRFTGRAEIACMHNAYHGSTHGALSMIGGSEMQKGFHPLLP
                     GINRLHFNRFDDLHLITHATAAVFVEPVQGEAGVRPANPFWLEALRKKCDETGTLLVF
                     DEIQTGYGRTGTLFAFQEYGIIPDILVLAKGMGGGMPLGAFMARSEIMDCLRRDPILG
                     HITTFGGHPVSCAASLAALEILLHSDILQQIPAKEELFRSLLVHEKILEVRGKGLILA
                     MEIGSFDYVQEVIHRCIEAGLISDWFLFCDSALRIAPPLTITEEEIKEACSILIQCL"
     gene            complement(3226..3882)
                     /locus_tag="PK610_09265"
     CDS             complement(3226..3882)
                     /locus_tag="PK610_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36166.1"
                     /translation="MIRKSFLYFFFVAVIVFSSRLCAQNGLSISATAELFTVASDLGA
                     GGDIEFQNTKFVYGGALSFSRDHGREVYYPLRIVEYQFNNYQLSGQFLYRFTQREKHG
                     GELGLCYTFFRSHADYLDWFPSFYKFQFNGNYDMHFAGIMMGGRQNISDRFFIRMHWR
                     YLLQIASFEYPDYKLVAIYDPNWTPDENMKKGRSYFSIKLGFGMMIRKKKKQLNPSDD
                     "
     gene            complement(3921..>4578)
                     /locus_tag="PK610_09270"
     CDS             complement(3921..>4578)
                     /locus_tag="PK610_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRJ36167.1"
                     /translation="VGGDILFIETILTPGKGKLSMTGNLGEVMKESAIIALEFLKAHS
                     SKLGINIKTFDQHNVHVHVPEGATPKDGPSAGITMLTALASQFTGRKVKKALAMTGEI
                     TLRGKVLPVGGIKEKILAARRAGIKEIILCEKNRKDVEDIKPAYIEGLTFHYVSDMME
                     VLDAALTNERSGSDKNSKVTKTAVKKTIAKKSAAKTTGAKKSVRKPVSAKKTKKKVRK
                     "
BASE COUNT         1441 a          996 c          868 g         1273 t
ORIGIN      
        1 attcctttaa cactccggat gcccaaaaca gaaccgatgg caataagaaa attgcgtaag
       61 ccatccactt ttcctttccg gtaaaaaaag atttaaacgc cttgtacaaa gccactaatc
      121 cggttaatga caaaaaacag gaaaacaagg tatgtacatg aaaagatcca aacgaaaata
      181 ttcgcaagac agcattgatt cggattatag tatgactatc gttgtaaata ttgctttcgt
      241 atttccgaaa ccaattgttc atgtggttat aatactgctc attgaaataa ttgttgtcgt
      301 tttcgtaccc aaacaacatt ctgaaaaaat ctcccggctt atggaatagc gcatctgtca
      361 taagtgcgct atcgtcaaaa tatttataga tatctgcatt cgcgcgatcg gtatagtaga
      421 atgtataaat aatagtgaga aagaaaccgg ccactacttt taaaagaaaa acataagtaa
      481 taaaccgggg ttgcaattcc tgaattcgaa aaaacgaatg actacggatc agccatagca
      541 tcaggaaaag ccagacagaa accagtaaag catcggatat actgaaaaaa ctcattgatg
      601 aaagataatg aatttaggaa acgacctcat tctctttttt tttttaagat ttcactaatg
      661 catttactac aacagatatt acaacaataa acattccgat aagactcata aaagtaaagg
      721 tctcatcaaa aaacaaatat cccaatagca gtgcatacac tgttccaaga tatttaaagg
      781 gggttattac agcagccttt tcagcatgta atgccatggt cataaacacc tgcgcaacct
      841 gcgtgaagag tccgatcagc aataaataca cccaatcgac accttgaggt tgaacccatt
      901 cattcattgc ccagataccg gtaattgggg cggcaataag tggaaatgcc attacaacgg
      961 tatacgggtg atcggtatcg cggcatttta tgattgcatt ataggcaact gcagataaaa
     1021 atgccgacaa aacacccaac caaagaaaag tccagttcgg tttttcctga taaccttcca
     1081 tcatcagcaa accggcaaac gaaacaacaa agaataccca acgaattggt ttcacttttt
     1141 cgcccactaa aaacatagca ataaccacag taaaaaccgg agacaaatac tgtacggtgg
     1201 tggccgtagc cattggcaat tctttcaagg tgtaaaaaaa cagtatcaat gccgaagccc
     1261 caaacacacc gcgaacaagc aaccaaaaat gattatttcc gaaaaagggt aaacctttat
     1321 gaataaccat acctgcagtt aacacaagcg tgataaggct gcgaaaaaaa actatttcag
     1381 acgttggaat atgatcgaga aacttaacca gaaaattgac cacagcaaat cccgcagttg
     1441 cagttagcat gtacaaaatt cccggtctga tcacatgcaa atgtattatt tcgaaacaca
     1501 tctggacaag gatcacctgt gcctacttgg ttttcaatac cattttacaa tactcaaagg
     1561 ggaatgatct caggctaaag cattattctg taaccatttc taatgctata agcagaagct
     1621 ctatcaaaaa ccccaggctg aagcattatg ccattatcgt tacttccagt actagaatta
     1681 cccctgacga aagccatgtc tcacctttga ttcttccgct attagcagat tcactatcaa
     1741 aaaccccagg ctgaagcatt attctattat cgttacttcc agttccagaa ttaacccgga
     1801 cggaaagctt gtctcatctt tgattcttca gctattagca gatgcactat caaaaacccc
     1861 aggctgaagc attattctat tatcgttact tccagttcca gaattaaccc ggacggaaag
     1921 cttgtctcat ctttgattct tccgctatta gcagatgcac tatcaaaaac cccaggctga
     1981 agcctggggt tacaaaatta ggaagcggta aaatcctcgg gctgaagccc gggattttga
     2041 gtagttttgg gttatgtctt cttatcgccg actttttttg gagcacctgg ctcaaacctc
     2101 agattttccg atggcagtag aaatcgcccg ggcagaaggg tgttacatgt atggtccgga
     2161 tggtaaaaaa taccttgatc tgattgccgg aatatccgtc agcaatctcg gtcacgggaa
     2221 tcccgctatt aaaaaagcga ttaaagagca ggttgacacc aatatgcacc tgatggttta
     2281 tggggaattt gtgcaggcat cacaagtcaa attagctacg gctcttcaca aagcaaccca
     2341 tcctacgctt acacaaacgt atttcaccaa ttcaggaagt gaagcgattg aaggggcaat
     2401 gaaactggca aaacgattta caggaagagc ggaaatcgct tgtatgcata atgcctatca
     2461 tggcagcaca cacggtgcat tgagcatgat tggtggaagc gaaatgcaaa aaggattcca
     2521 cccattatta cccggaataa acagacttca ttttaatcgg ttcgacgatt tgcatctcat
     2581 cacgcatgca acagcagccg tttttgtgga acctgtgcag ggcgaagcgg gtgttcgtcc
     2641 ggctaaccct ttctggctag aagctctgcg taaaaaatgt gatgaaacag gaaccttact
     2701 tgtgttcgat gaaattcaaa ccggatatgg tagaactggg actttgtttg catttcagga
     2761 atacggaata atcccggata ttttagtcct cgcaaaaggc atgggtggag gaatgcctct
     2821 tggtgcattt atggcaagat ctgaaataat ggattgttta cggagagatc caatccttgg
     2881 tcacattacc actttcggag gtcaccccgt ttcgtgtgca gcaagtcttg cagcattgga
     2941 aattttactt cactcagata tccttcaaca gattcccgct aaagaagaat tattccgcag
     3001 tttacttgta catgaaaaaa tacttgaagt ccgaggaaaa ggcttgatac tggcaatgga
     3061 aattggttcc ttcgactatg tacaggaagt catacaccgt tgcattgaag caggtttgat
     3121 ctctgactgg tttttgttct gcgattcggc attgcgaatt gctcctccgc ttacaattac
     3181 cgaggaagaa attaaagaag cttgttcaat tctgatccaa tgtctttaat catcggacgg
     3241 atttaattgt ttcttttttt ttctgatcat cattccaaat cctaatttga tagaaaagta
     3301 acttctcccc tttttcatat tctcatccgg tgtccaattg ggatcataaa tagccaccaa
     3361 cttataatcg ggatattcaa agcttgcgat ctgcaataaa tacctccaat gcattcggat
     3421 aaaaaagcgg tcagatatgt tttgtcgccc tcccatcatt atccctgcaa aatgcatatc
     3481 ataatttcca ttgaactgaa atttataaaa tgaaggaaac cagtcaaggt aatcggcatg
     3541 actccgaaaa aaagtataac aaagacccaa ttcacctcca tgtttttccc gctgcgtaaa
     3601 gcgataaaga aattgaccac tcaactggta attattgaat tgatattcaa caatacgtaa
     3661 aggataatat acttcccttc catggtcgcg tgaaaacgat aaggctccgc cgtagacaaa
     3721 ttttgtgttt tggaattcaa tatcgccccc agcccctaaa tctgaagcaa cagtaaaaag
     3781 ttctgccgtt gctgaaatgg acaagccgtt ctgcgcacag aggcgcgatg aaaacacaat
     3841 cacagcaaca aaaaaaaagt acagaaatga tttgcgaatc atctctgtac taacgaaaaa
     3901 ataaattatc tattgtgtgc ttacttccgc actttctttt ttgtcttttt agctgaaaca
     3961 ggtttacgaa ctgacttttt tgcgccggta gttttggcgg cacttttctt tgcaattgtt
     4021 tttttcacag cagttttggt gaccttgcta tttttatcag acccggagcg ttcattcgtt
     4081 aatgcggcat ccagcacttc catcatgtcg ctgacataat gaaatgtcaa accttcaatg
     4141 tatgctggct taatatcctc aacgtccttt ctgttctttt cgcaaagaat aatctctttt
     4201 atacctgcgc gtcgtgctgc aagaattttt tctttgatac caccaacggg caatacctta
     4261 ccacgaagcg tgatttcgcc ggtcatggcc aatgcctttt tcactttacg ccctgtaaac
     4321 tgcgacgcaa gtgctgttag catagtaatt ccagcagacg gaccatcctt tggtgtagca
     4381 ccttcgggaa cgtgaacatg aacattatgc tgatcaaatg tcttgatatt aattccgagt
     4441 ttggaggaat gagcttttaa aaattccagc gcaatgatcg cggattcttt cattacctca
     4501 cccagatttc cggtcatgct caactttcct tttcctggag taagaattgt ttcgatgaaa
     4561 agaatatcac ctcctacg
//
LOCUS       DAOMYG010000272         4548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6661_length_4548_cov_13.068774, whole genome shotgun sequence.
ACCESSION   DAOMYG010000272 DAOMYG010000000
VERSION     DAOMYG010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6661_length_4548_cov_13.068774"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PK610_09275"
     CDS             complement(<1..1033)
                     /locus_tag="PK610_09275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRJ36168.1"
                     /translation="MRTKLILLAAIVLSSVSGLIAQDIHFSQFYMTPVQFNPSYAGAL
                     KELDVSMQYKGQWNMIDKGYRTLGATVSSRINYKRGNPNFWAIGLTAFADQAAINKLN
                     SLRINIPLCYTVKLNQLNSLSNAVYVGFGQNVLKDPNLTWGSQFDGMNYNSSLPTNET
                     QFTQSFRFLDMGYGFSWNHNQQTRTVADEKGFSNSLGFSVGHLNRPMYSFNNNPSDKL
                     DMKFSLYNSSSIYIKNSKISWHPSVLVQLQGGHREIMYGALCRYEIQQESRITGFREG
                     SAISAGMYRRSKDAIVIAGLYEINHFQIGMSYDMNTSALSNSTGYRGGFEILLKYITP
                     SPFSGGGRSI"
     gene            complement(1036..>4548)
                     /locus_tag="PK610_09280"
     CDS             complement(1036..>4548)
                     /locus_tag="PK610_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ36169.1"
                     /translation="LFISVYAQSQGQNYNWHFGAGLRLNFPGSVGPIFLTGSALGTEE
                     GCASISDAAGNLLFYTDGKFVWNQNNIQMPNGFGLLGGISGSSSQAAIIVPQPGSSTM
                     YYIFTVDEDGGAGGFRYSIVDMSLNTGLGDVVTKNVLIHTPSCEKIAATYHCNGTDVW
                     IVTHDLNSNQYRAVLLTAGGVGAPVVSALGPVIGSTLGASGAGCLKFSHDGTKAVITN
                     MINPGLLDLIDFDNSTGTFSNRRALYTPAEQDGLYGIEFSPNNQLVYISNHDIDEVHQ
                     YDISSGVAASILASRTVLGTTSVGGNFGQIQIGPDGKIYFIQTRTTFVGCIQSPNVAG
                     AGCNWTELYYDTDPLATGLYDIRFGLPSQLTYIPTQFSYPTNSYCQSDPAIPAPSFVS
                     TYPVTGTFTSSPAGLSINPTTGVIQLSTSAAGTYTIRYTAAVASSCVSPFYSEFTLTV
                     LPAPVVNITGDNTVCENVNVVLTASGGTSYSWTGPSWQGGGANSSSATLTNPGNATLG
                     QSNEGWYYVNVSGGGCTSRDSIFIDILAVPSVSISNIPTACTNDLTATINSSNGGISG
                     YEWTSNGIPPVLGTNSTLSYNPFAPDQIVVTVTDNEGCIGSASLVVNPISPLNISYSG
                     GPFCTNGTPIPVSLTGTPGGTYTTTMGLTINSTTGEIAPTTSTPGTYTVTYTQGVCST
                     TTNVTINAAPTATLVYPSATICGLATSFIPSNSGTPGGVFSSTAGVTLNTSTGVINPS
                     TSTPGTYTITYTIAASGSCPSVSDQFTVTIDAPTTPVVSFSFDPSYCTSATNPLPVLA
                     VGFTSGGTFSSSAGISINASTGLIDLAASTAGTYSITYSVSASSPCIAAGTFSTSVTL
                     IAPPVVSITGNTTICVGEQTTLTASGASTYQWTAGSSSTSAVITDQPGSSQTYTVIGT
                     QSGCSSTASVLVNVLPVPIASVSGNLNICEGQSVTLNASGGNSYVWSGGQNTSAITIS
                     PTSTQNLYVVAINGSCTDTAFVTINVNPIPSLSATATPPVITLGESSQLNANSAGSTF
                     SWTPSSSLSCSTCPDPVATPNSSTTYLVTATNSGGCTATATVVVIVDIVCGEIFTPEA
                     FSPNNDGNNDEWCVYGNCITELDVQIFDRWGNMVRRMSSASDCWNGEVNGKIADQGVF
                     MFQLNAKLLNGETVERKGSIYLMK"
BASE COUNT         1251 a          977 c         1043 g         1277 t
ORIGIN      
        1 aaatcgatcg tcctccacct gagaatggag aaggagtgat gtacttcaaa agaatctcaa
       61 atccacctct gtaaccggta gaattcgata aagctgaagt gttcatatcg taactcattc
      121 cgatttgaaa gtgattgatc tcgtacaaac ctgcaattac gattgcgtct ttacttcttc
      181 tgtacattcc cgcagaaata gcggatcctt ctctgaaacc tgtaattcgg gactcctgct
      241 gaatctcata tctgcacaat gctccgtaca taatttcccg atgtccgccc tgcagttgaa
      301 cgagaacaga aggatgccag ctgatctttg aatttttgat gtaaatggat gaactgttgt
      361 acaaagaaaa tttcatatcc agtttatcgc tcgggttgtt gttgaaggaa tacatcggac
      421 ggttaaggtg tcccaccgaa aatccgagtg aattcgaaaa tcctttttcg tccgcaacgg
      481 ttctggtttg ctgattgtgg ttccatgaaa aaccataacc catgtccaaa aaacgaaagc
      541 tttgggtgaa ttgcgtttca tttgtaggaa gcgaagaatt gtaattcatt ccatcgaact
      601 gactccccca ggtgagattg ggatctttaa gcacattttg accaaagcca acgtaaactg
      661 cgttcgaaag ggagttcagc tgattgagtt taaccgtata acacaaagga atgttgattc
      721 gaagcgaatt cagtttgttg atcgctgcct gatcggcaaa tgcggtaaga ccaatcgccc
      781 agaaattcgg atttcccctt ttgtagttaa tcctggaact taccgttgca ccaagtgtgc
      841 ggtaaccttt gtcgatcatg ttccactgac ctttgtactg catagaaaca tccagttctt
      901 tcaaagcgcc tgcataggat ggattgaact gaacaggcgt catataaaat tgcgagaaat
      961 gaatatcctg cgcaatgagt ccgcttaccg aactcaaaac aattgctgcc aataaaatta
     1021 atttcgttct catacttatt tcattaagta aattgatcct ttgcgttcaa ccgtttctcc
     1081 attgagaagt tttgcgttca actgaaacat gaacactccc tggtcggcta tttttccgtt
     1141 gacttcccca ttccagcaat cgcttgcaga gctcatgcga cgaaccatat ttccccagcg
     1201 atcgaaaatc tgaacatcca attctgtaat acaatttcca tacacacacc actcatcatt
     1261 gttgccatcg ttgttcggac tgaatgcctc tggagtgaaa atctctccgc aaacaatgtc
     1321 tacaatcacc actacggttg cagttgcagt acatcctccc gaattggttg ctgtaaccaa
     1381 ataggtggtg gatgaattgg gcgtggcaac aggatcagga caagtggaac aactcagcga
     1441 ggacgaaggt gtccagctaa atgttgaacc tgcactattt gcattgagct gtgaactttc
     1501 tccaagagta attaccggtg gagtagcagt cgctgataat gatggaatcg gattgacatt
     1561 tattgttaca aatgcggtat cggtacaact cccgttaatg gcaacaacat acagattctg
     1621 ggttgatgtc gggctgatgg tgatggcact tgtattttgt ccaccggacc aaacgtacga
     1681 attaccacca gatgcgttta gtgttacgct ttgaccttcg caaatattca ggtttccgct
     1741 aaccgatgca atcggaaccg gaagcacatt cacaagcacg cttgcagtag atgagcatcc
     1801 cgattgagtt ccgataacag tatacgtttg tgaacttccg ggctggtcgg tgattactgc
     1861 tgaggttgat gaagatcctg cagtccattg atacgtactc gctcctgatg cagtaagtgt
     1921 tgtctgttca ccaacgcaaa tggttgtgtt tcctgtgatc gaaacgacgg gtggtgctat
     1981 cagagttaca gatgtcgaaa atgttcctgc agcaatacaa ggtgaacttg cggaaacaga
     2041 ataggtaatt gagtaggtcc ctgctgttga tgctgccaga tcaatcaatc ctgtgcttgc
     2101 attgatgcta attcctgcac ttgaactgaa tgttccccca cttgtaaatc ctaccgctaa
     2161 aaccggaagt ggatttgttg cgctggtaca ataggaagga tcaaacgaaa agctcacaac
     2221 aggagtggta ggagcgtcaa tagtaacggt gaactgatcg cttactgaag gacatgatcc
     2281 acttgctgca atggtatagg taatagtata ggttcccggt gtagatgtag agggattaat
     2341 tacaccagta cttgtattta gtgttacacc tgccgttgaa gaaaatactc caccgggtgt
     2401 tccgctgttg ctcggtataa aacttgttgc aagtccgcaa atagttgcag atggataaac
     2461 tagtgtggca gtgggtgcag cattaatagt aacattggtt gtcgttgagc aaactccctg
     2521 tgtgtaggta accgtgtatg ttccgggcgt ggatgttgtt ggtgcgattt ctcccgttgt
     2581 tgaattgatc gttaatccca ttgttgtggt ataggttcct ccgggagttc cggtaagtga
     2641 aacaggtatc ggtgttccgt tggtgcaaaa tggtccgccg gaatacgaaa tgtttaaagg
     2701 agaaattgga ttaaccacca gagaagcact tccaatacaa ccttcattat ccgttacagt
     2761 aacaacaatc tgatccggag caaacggatt gtagcttaaa gtggaattcg ttcccaaaac
     2821 aggcgggatt ccgttgctgg tccattcgta tccggaaatt cctccgttcg aactattgat
     2881 tgttgcagta agatcgttgg tgcatgctgt tggaatattg cttatcgata cacttggtac
     2941 agcgagaata tcaataaaaa tactgtcgcg cgatgtgcag cctcctcctg atacattgac
     3001 gtaataccaa ccttcattgc tttgtcccag tgtggcgttt ccgggattgg taagtgttgc
     3061 tgatgatgaa tttgctccac cgccttgcca gcttggaccg gtccatgaat aggaggttcc
     3121 tccgcttgca gtaagaacta catttacatt ttcacatacg gtattgtctc ctgtgatatt
     3181 tactaccggt gccggtaaaa ccgtaagtgt aaattcgctg tagaaaggag aaacacagga
     3241 tgaagcaaca gctgcagtat atcggatggt atatgtaccg gcagcggagg tgctcaattg
     3301 aatgactcct gttgtaggat ttatggacaa tcctgccgga ctcgatgtaa atgttccggt
     3361 taccggataa gttgaaacaa atgatggtgc aggaatagca ggatcgcttt ggcaatagga
     3421 attggtggga tacgaaaatt gtgttggaat ataggtgagc tgactgggta atccaaaacg
     3481 gatatcgtac aaacctgtgg caaggggatc ggtgtcgtaa tacaattcag tccagttaca
     3541 tcctgcacct gcaacatttg gagattgaat gcatcctaca aatgttgtgc gtgtttggat
     3601 gaagtaaatt tttccatctg gaccaatctg gatttgtccg aagtttcctc caaccgatgt
     3661 tgttcccagt acagttcgtg atgctaaaat gcttgctgca acgcccgaac tgatatcgta
     3721 ttgatgaact tcgtcaatat catgattact gatgtagacc aactgattgt ttggagaaaa
     3781 ttcaataccg tataaaccgt cttgttcggc aggagtatac agagcacgac ggttggaaaa
     3841 tgttcctgtg gaattatcaa agtcgatgag atccagcaag cccggattta tcatattggt
     3901 gataacggct ttcgttccgt cgtgcgaaaa ttttaaacac ccggctccgg atgctcccag
     3961 tgttgaaccg ataacaggac ccaatgcact taccaccggt gcaccaacgc ctcctgctgt
     4021 gagaagcact gctctgtatt gattggagtt tagatcatgt gtgacaatcc atacatcagt
     4081 tccattgcaa tgataggttg ctgcaatttt ttcgcaggat ggtgtatgga tcagaacgtt
     4141 ctttgttacc acatcaccta atccggtatt cagactcatg tcaactatcg aatagcgaaa
     4201 tccaccagcg ccaccgtctt cgtctacggt aaaaatgtaa tacatggttg aggaaccggg
     4261 ttgcggaaca ataatagctg cctgactgga tgaacctgaa attcctccaa gcaatccgaa
     4321 tccgtttggc atttgaatgt tattctgatt ccagacaaat tttccgtcgg tgtaaaaaag
     4381 aagatttcct gcggcatcag aaatactggc acacccttct tccgttccaa gcgcagatcc
     4441 cgtaaggaaa atagggccaa cacttcccgg aaaattaagg cgtaaaccag caccaaaatg
     4501 ccagttgtaa ttctgcccct gagattgcgc gtacaccgag atgaataa
//
LOCUS       DAOMYG010000273         4529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6706_length_4529_cov_9.626956, whole genome shotgun sequence.
ACCESSION   DAOMYG010000273 DAOMYG010000000
VERSION     DAOMYG010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6706_length_4529_cov_9.626956"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1385
                     /locus_tag="PK610_09285"
     CDS             132..1385
                     /locus_tag="PK610_09285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ36170.1"
                     /translation="MKNILTILFVTGFLFNSSAQTVTDTVSIGAAYSNQVWYSLHNNE
                     QATDPINNWEIAIAATSNQVNPLTTAIHFNFKIGSLYEIPGSSPANFATADTSGINSW
                     TALYNSDNTWAEGAFNNTTSLGGFDYGWGSYNSVTHNVDPNRVFVIRYNTTPVSYKKL
                     HIGLNAMAGVYTITHANLDNSNQLSQQITVSTYSSKNFIYYSLSTSNIIDREPAKTSW
                     DLLFGQYQSFDYNPPYTVSGILNNHGIESVKVYPVNNPVSYTNWGAQTFTQNISEIGY
                     DWKAFAGVWTIEDSTVYFVKDVSGDIWKIIFTGFGGSTNGNYIFTKQKLSSVGIDETG
                     GSLTIDVFPNPNDGQFIIVGNGTEDPFNIIIMDLTGREVYNEIIATGNGLFKRNIVLS
                     GITPGVYLMNLIQGERMISKKIVIR"
     gene            1390..2256
                     /locus_tag="PK610_09290"
     CDS             1390..2256
                     /locus_tag="PK610_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ36171.1"
                     /translation="MRKLILILCSALTVSCSTPARSTEETTEAPKERIVSLNGTLTEI
                     LCASGLESSIVGTDVTSAFPESIQKLPKLGHVKSLNAEGIIALSPTMVFGKKDEIKPE
                     LEKQLIEAGIKVLLFEQEYSVDGSKRLIKEVCATVNAEESGKKLITKIDEDLKGISSM
                     THVPRVLFIYARGAGTLMVAGDGTQMQKMISLSGADNAVNGFPDFKPLTPEALLEANP
                     DVILMFESGVESLEGENGIWSIPGMADTKAGKTKQLVVMDGLYVSGFGPRLGQAVTEL
                     NKKLISVNQTSN"
     gene            2258..3298
                     /locus_tag="PK610_09295"
     CDS             2258..3298
                     /locus_tag="PK610_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRJ36172.1"
                     /translation="MRKPTHILLVLAVLLLGSIVASCALGAVKVHPTEFVSILLNKFG
                     WSDYNSFSAQQEAVIWVIRLPRVITSVLSGALLALSGVAIQGLFRNPLADPGIIGISS
                     GAAMVASIAIVISGTFSFLQGTFAGISFLVIAAFAGSLLSAAGVFRIAREKTKTNVST
                     MLLAGIAINAIAGAVTGILIYISNEQQLRDLTFWTLGSLGGSTWTNAGILLVLLVVTY
                     LVFFRLSKALNAFSLGEQEAQHIGISIESAKNRIIIFSSIAVGATVAFNGIIGFVGLV
                     VPHILRITGSYNHRFLIPAAALGGGLLLSLSDLAARTLVAPAEIPVGIITALAGAPVF
                     LFILLQQKHTFR"
     gene            3298..4140
                     /locus_tag="PK610_09300"
     CDS             3298..4140
                     /locus_tag="PK610_09300"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HRJ36173.1"
                     /translation="MLEVRNISYGVRGKAILSNISFSVGPGKLVAILGPNGAGKSTLL
                     KIISRELNALQGTIFFSGKELQSYSVEQLSLQRAVLTQSIQLSSRFEANEVVMMGRYP
                     HFESFPSEKDKAIVNEVMQMTETYPYNDRIYQELSGGEQQRIQFARVMTQVRGENVFN
                     RMLLLDEPLNNLDVRHQYKLLSIASEFSKNGNTVLTVLHDINLAAMYADEVILMAKGH
                     VHSIGLPKDVITGENITSCYDFPARVESHPFHQCPVVYFGCPEETKTDSFFQQTELIT
                     TSNL"
     gene            4149..>4529
                     /locus_tag="PK610_09305"
     CDS             4149..>4529
                     /locus_tag="PK610_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChuX/HutX family heme-like substrate-binding
                     protein"
                     /protein_id="HRJ36174.1"
                     /translation="METKTQSLRERWAELKESNPHLRIRNAAEKLGVSEAELLASQVG
                     EKLPNGEIAVIRLKPEFQTILGRIEELGKVMGLTRNDAVVHERKGVYLNPSLNSPAIG
                     LFVGEDIDLRIFFSCWKSAFAVNEP"
BASE COUNT         1358 a          903 c          967 g         1301 t
ORIGIN      
        1 ttaaatcaaa tttttaaaat acccttttca tggtatttta tttagaatca ttctaaatac
       61 ctgaataatg gtattgctga tcatccacct atgttagact tttggttcca ggaaaatcaa
      121 aatgaaaaac gatgaaaaat atcttaacaa ttctttttgt taccggattt ttattcaatt
      181 cttctgcaca aactgtgacg gacacagtga gcattggagc tgcttatagt aatcaggtct
      241 ggtatagtct gcacaacaat gaacaagcaa ctgatccaat caacaactgg gaaattgcca
      301 tagcagctac atcaaatcag gttaatcctt taaccaccgc tatccatttc aactttaaaa
      361 taggatcact ttatgaaata ccgggatcat cgccagcgaa ttttgcaacg gcagatacca
      421 gtggaattaa ctcctggact gcactttaca acagtgacaa tacatgggcg gagggagcgt
      481 ttaacaatac cacttctctt ggtggttttg attatggttg gggatcttat aattcagtaa
      541 cacataatgt agatcctaac cgtgtttttg tgatccggta caataccaca cctgtgagtt
      601 ataaaaaatt gcatattggt ttgaatgcca tggcgggagt gtatacgatt acacatgcca
      661 acttggacaa ttcaaatcaa ctaagtcagc agataactgt aagtacatat tcatctaaaa
      721 actttatcta ttactctctt tccaccagca acatcatcga ccgcgagcct gctaaaacaa
      781 gttgggattt attgttcggt caatatcaat cgttcgacta caatcctcct tacacagtat
      841 ccggaattct gaacaatcac ggtattgaat cagtaaaggt gtatccagta aataatcctg
      901 tttcttatac caattggggc gctcaaacct ttacgcaaaa catcagcgaa atcggctacg
      961 actggaaagc attcgcaggt gtatggacaa ttgaagattc gactgtatat tttgtaaaag
     1021 acgtatccgg tgacatctgg aaaatcattt tcaccggatt tggtggaagc accaacggaa
     1081 attacatttt cacaaagcaa aagcttagca gtgttggcat tgacgaaaca ggtggctccc
     1141 tgacaataga tgtttttccc aatccgaacg atggacagtt tatcattgta ggtaacggaa
     1201 ctgaagatcc gttcaacatt ataattatgg acttaactgg ccgggaagtg tacaacgaaa
     1261 taattgccac gggaaacgga ctgttcaagc gcaatatcgt gctttcagga attactccag
     1321 gtgtatacct catgaatttg attcagggcg aaagaatgat ttccaaaaaa attgtaatcc
     1381 gataattcca tgcgaaaact tattttaatt ctctgttctg cgttgacagt atcttgttcc
     1441 acaccggcaa gatcaaccga agagacaaca gaagcaccaa aggaaagaat agtatcgcta
     1501 aacggtactc tcacagaaat cttatgtgca tccggtcttg aaagttcaat tgttggaact
     1561 gatgtaacaa gcgcttttcc ggagagtata cagaaacttc ccaaactcgg tcatgtaaaa
     1621 tcactgaatg cagaaggtat aattgcactt tctcctacga tggtattcgg gaaaaaagac
     1681 gagataaaac ccgaacttga aaaacagtta atagaggctg gaatcaaggt actcctattt
     1741 gagcaggaat attctgttga tgggagtaag cgattgataa aagaggtgtg tgctactgtg
     1801 aatgcagaag aatccgggaa aaaacttatc accaaaattg atgaggatct gaagggtatt
     1861 agttcaatga cgcatgtacc acgtgtgctt ttcatctacg cccgcggtgc tggtacattg
     1921 atggttgccg gagacggaac ccagatgcag aaaatgatct ccttatcagg tgccgataat
     1981 gccgtgaacg gatttccgga tttcaaacct cttacacctg aagcactgtt ggaagccaat
     2041 ccggatgtga ttctgatgtt tgaaagtgga gtggaatccc tggaaggaga aaatggcatt
     2101 tggtctattc caggaatggc agatacaaag gcaggaaaaa ccaaacagtt agtagtaatg
     2161 gacggtcttt atgtatcggg atttggtcca cgccttggtc aagccgttac ggaacttaat
     2221 aagaaactga tttccgttaa tcaaacaagc aattaaattg cgaaaaccaa cacacatact
     2281 tttggtactc gctgttttgc ttctcggaag cattgtggcg agttgcgcat tgggtgcagt
     2341 gaaagttcat ccgactgaat ttgtttccat tctattgaac aaattcggtt ggagcgatta
     2401 taattctttt tcagcgcaac aggaagcggt aatttgggta attcgattgc cgagagtaat
     2461 aaccagtgtg ctttccgggg cactattggc gctaagcggt gttgctatac agggtttatt
     2521 ccgcaatcca ctcgcagatc ccggtattat tggaatttct tctggtgcgg caatggttgc
     2581 atccatcgcc attgtaattt caggaacctt ttcatttttg caaggtacct ttgcgggaat
     2641 ctcatttctg gttattgcag cttttgccgg atcacttctc tctgcagcag gtgtttttcg
     2701 gattgcacgc gagaaaacca aaactaatgt atccactatg ctactggcgg ggattgctat
     2761 taatgcaatt gcaggtgcag taacaggtat tttgatctac atttcgaacg aacaacaact
     2821 acgcgatctg accttttgga ctctcggaag tttaggcgga tcgacctgga cgaatgcagg
     2881 aattctgttg gtgttgcttg ttgtcactta tttggtgttt ttccggctta gcaaagcatt
     2941 gaatgcattt tcacttggtg aacaagaagc acaacacatt ggaatttcca tcgaaagcgc
     3001 caaaaacagg atcatcattt tttcatctat tgcagtgggt gccactgttg ccttcaacgg
     3061 tattatcggt tttgtgggac ttgtagttcc tcacattcta cgcattaccg gaagttacaa
     3121 ccatcgtttt ctcatccctg cagctgcatt gggaggagga ttattgttat cgttatcaga
     3181 tctcgcagca cgaacacttg ttgcaccggc agaaattcct gttggaatta taactgctct
     3241 tgctggagca cctgtatttt tattcatact tctgcaacaa aaacacacat tcagataatg
     3301 cttgaggtca gaaatatctc ttacggcgtt cggggaaaag ccattctcag taacatttcc
     3361 ttttctgtag gtccaggtaa acttgttgct attcttggtc cgaatggagc aggaaaatcg
     3421 actctgttaa aaatcatcag ccgcgaactt aatgcattgc aggggaccat tttcttttcc
     3481 ggaaaggaac tacaatctta ttccgttgaa caattatcgt tgcagcgtgc tgtacttaca
     3541 caaagtattc agctaagcag cagatttgaa gcaaatgaag tggtgatgat gggtagatat
     3601 ccacattttg aatcttttcc ttcagaaaaa gataaggcta tagtaaatga agtaatgcag
     3661 atgacagaaa cgtatcctta caacgaccga atttatcagg aactatcagg tggtgaacag
     3721 cagcgcatac agtttgcccg cgtaatgaca caggtacgcg gtgagaatgt tttcaaccgc
     3781 atgttattac tggatgagcc tctcaacaat ctggatgttc gccaccaata caaactattg
     3841 agcattgcat ctgagttttc caaaaacggc aacacggtat tgacggttct tcatgacatc
     3901 aatcttgctg caatgtatgc agatgaagtg attctgatgg cgaaaggcca cgttcattct
     3961 atcggcttac cgaaagatgt aataacggga gaaaacataa catcctgtta tgactttccg
     4021 gcgcgggtag aatctcatcc ttttcatcaa tgtcctgtag tttatttcgg ctgccctgaa
     4081 gaaactaaaa cagattcttt ttttcagcaa acggaactta taacaacatc taacctataa
     4141 tactaaaaat ggaaacgaaa acacaatcac tcagagagcg atgggctgag ttaaaggaaa
     4201 gcaacccaca tcttaggatc cgcaacgctg cagaaaaact tggagtcagt gaagcagaac
     4261 tattggccag ccaggttggt gaaaaactgc ccaatggaga aattgcggta attcggctga
     4321 aacccgaatt tcagactatc cttggccgca ttgaagaact tggaaaagta atgggtctga
     4381 cacgaaatga tgctgttgtt catgaacgga aaggtgttta tttgaatccg tcgctaaata
     4441 gtcctgctat tggattattt gttggtgagg atatcgatct cagaattttc ttcagttgct
     4501 ggaaatctgc atttgcggta aatgaacct
//
LOCUS       DAOMYG010000274         4518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6735_length_4518_cov_7.311002, whole genome shotgun sequence.
ACCESSION   DAOMYG010000274 DAOMYG010000000
VERSION     DAOMYG010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6735_length_4518_cov_7.311002"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PK610_09310"
     CDS             complement(<1..721)
                     /locus_tag="PK610_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010177510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRJ36175.1"
                     /translation="MKLQLRRPICFVDLETTGVNVGSDRIVEISILKKKPDGSSELFT
                     RRVNPEMPIPAESSAIHGITNKDVATAPTFEQLAPEVHSFIGDSDLAGYNSNKFDFPM
                     LVEEFLRCKIDFDLRTRKMIDVQAVFHKMEQRTLQAAYKFYCEKELVNAHSAEADILA
                     TAEVLDAQLERYPDLTNDVDYLHEFTKPKFEPLDFAGRIVLNDKKQAVFNFGKHKGKT
                     VEEVFRVEPSYYNWMMQGDFPL"
     gene            complement(729..2255)
                     /locus_tag="PK610_09315"
     CDS             complement(729..2255)
                     /locus_tag="PK610_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36176.1"
                     /translation="MILLAGTMFLMGPAFAQPDPGIADDFFKKTNYVYAIPEYKTLLK
                     LNRDHPEYNFKLGVCYLRTNIDRAAAVPYLERAYKQSKYPAETPYYLAVAYTHVYKYD
                     QALELFREYRKKAPSKDHPMIDRYIENCQVAKEMMRNPIPITFQNLGPGVNSEFPDYY
                     PFTTADEKTIYYTSRRKEGKSSKVEYDGYYASEIFSFTYNGESFTPGKPQANLNSLYD
                     DQVVGLSADGENVFVFSTATENKGALYRCYKKGGQFKKEMFIQQVNNEKSIETTGFMS
                     PDGNIIFFASNRSGGYGGYDIWLVRKLPNGQWGMPFNCGPEINTPFDEDFPTLSFDGL
                     TLYFSSNGHPGMGGFDLYQSNFDPETGMFSQVKNIGYPLNTPADERTISFAEDGKHAY
                     ISASRKDSQGDLDIYRVTFEDVEFIQTLFTITIPVPGTSDQLIKDAIITVSNEAGDVL
                     GDYRPNPNTGRFTIILGPGKYEFSVEMAGYKPYSEKVKVNEFTHRLGKIEKIFNLTPE
                     "
     gene            complement(2414..>4518)
                     /locus_tag="PK610_09320"
     CDS             complement(2414..>4518)
                     /locus_tag="PK610_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ36177.1"
                     /translation="EGSTGGQLFYDSKYFEDIYASPKADSLQFAFPTKFDSAGIYISS
                     KINTKTHDAAIGFNADETKLFIYRDNDIWQSEQINGVWTDPVRMNRNVNTKQHEPSIH
                     ITPNEKILFIVSTREGGFGGRDLYVSYQQEDGTWGPAINLGEDINTPEDEDAPFLSSD
                     LQTFYFSSKGHNSIGGYDIFKCPHSNGVFGDPINMGIPINSAGDDIYFHVDINNTFGY
                     FASSRVGGYGDMDIYRIQLGCKDIPNTELRGTILAGERQLPAFGRITIKEKSSGKIVG
                     NYTSNPYSGRYVIVLPPNNTYDMEVEVEGFEAGDVHKQTFTLPKQCEFFPLYQEINIR
                     RVRDSIENAMAQESKFRNAFFNLNNEITSTYALRKLPESESGIDQLQNPDHYLMISGN
                     IRHNSVIPGKGLIIYLVNSKNEIIYSTTSDEHGFFQFMFIDPKEPYKILVDETSARIS
                     YFGDTKEGATAEITLEGRLEQTDLRSRNTTGLANTEIYFVDDNKQITHLSKTNDKGNF
                     KFGKKADLNKDLNRLNQEMAFIYRIKADDINILYGSYIRTIQPGKTGLEYSEFIDRLF
                     LEKLAKEVPAFENIYFDFDKFFLRNRSVEILDIIYAFMVANPEVTIAIDGHCDNIGTE
                     DYNMGLSSRRSNSTYTFLVSKGIAKSRIKSAWHGESRPAAANAYPDGKDNPEGRQLNR
                     RVEFKIDIPGMAQVTLSY"
BASE COUNT         1216 a          931 c          978 g         1393 t
ORIGIN      
        1 aaagcggaaa atcaccctgc atcatccaat tgtaataact gggttccacg cgaaatactt
       61 cttcaacggt ttttcccttg tgtttgccaa aattaaagac agcttgcttt ttatcattca
      121 gaacaattcg tcctgcaaaa tcaagcggtt caaatttcgg tttggtgaat tcgtgaaggt
      181 aatcgacatc attggtaaga tcaggatatc gctccaactg cgcatcgagc acttcggcag
      241 tggccaggat gtcggcttct gcgctatggg cattgaccaa ttctttctcg caataaaatt
      301 tataggcggc ctgcagggtg cgttgctcca ttttatgaaa tacggcctgc acatcaatca
      361 tttttcgtgt gcgtaaatca aaatcgatct tgcagcgtag aaattcttcc accagcatcg
      421 ggaaatcgaa tttgttggaa ttgtagcctg ccagatcact atctccaatg aaggaatgca
      481 cttcaggagc cagttgttca aaggttgggg ctgttgcaac atctttattg gtgataccgt
      541 gaatcgcgga agattcggcc ggaatgggca tttcaggatt tactctgcga gtaaagagtt
      601 cagaactgcc atcaggtttt tttttcagta tcgagatctc cacaatgcgg tcgctgccaa
      661 cattaactcc ggtggtctca agatctacaa aacaaatggg gcgacgtaat tgtaatttca
      721 ttcgataatc attctggagt aagattaaag attttctcga tcttacccaa acgatgagtg
      781 aactcattca cctttacctt ttcactatat ggtttgtatc ctgccatctc aacgctgaat
      841 tcgtatttac ccggaccaag aatgattgta aatcttccgg tgttggggtt cggacgataa
      901 tcacccaata catcacctgc ttcgttgcta acggtaatga tcgcatcctt aatcaactga
      961 tcagaagttc cgggaacagg aatggtaatg gtgaatagcg tttgaatgaa ttcaacatct
     1021 tcgaaggtaa ccctgtaaat atccaaatcg ccctgactat cttttcttga tgcagagatg
     1081 taagcatgct tcccatcttc tgcaaacgaa atcgtacgct catcagcagg agtattcagc
     1141 ggataaccga tgtttttcac ctgtgagaac attccggttt cgggatcgaa attgctttgg
     1201 tacagatcga atcctcccat tcccggatga ccattcgaac taaaatacaa tgtcaaacca
     1261 tcgaacgaca gcgttgggaa atcctcatcg aaaggagtgt tgatctccgg accgcaattg
     1321 aagggcattc cccattgtcc gttgggtagt ttacgcacca gccagatatc gtaaccacca
     1381 tatccgcctg aacggttgct ggcaaaaaag atgatatttc catcgggcga cataaacccg
     1441 gtggtttcaa ttgatttttc gttgttgact tgttgaatga acatttcttt tttgaactgt
     1501 ccgccttttt tgtaacacct gtacaatgca cctttgtttt cggtggctgt agaaaacaca
     1561 aatacatttt caccatcggc cgataatccc accacctgat catcgtagag cgaattcaaa
     1621 ttggcctgag gttttccggg agtaaaactt tctccgttgt aggtgaaact gaaaatttct
     1681 gaagcgtagt acccgtcgta ttcaactttg gaagatttac cttcttttct tcgggatgta
     1741 taataaatcg ttttttcgtc ggcagtggta aagggataat agtccgggaa ttcggaattg
     1801 acaccgggtc caagattttg aaatgtaatt ggaataggat tccgcatcat ttccttcgca
     1861 acctggcagt tttcaatata gcggtcgatc atgggatgat ctttcgaagg tgcttttttg
     1921 cggtattccc tgaagagttc gagcgcctga tcatacttat agacatgcgt ataggcaacg
     1981 gcaaggtagt aaggtgtttc tgcaggatat tttgactgtt tgtaggccct ttccaaatag
     2041 ggaacagcag cagctctgtc gatgtttgta cgcaaatagc aaactcccag tttgaaatta
     2101 tattccggat gatcgcggtt taacttgagc aaagttttgt attcgggaat agcgtagaca
     2161 tagtttgtct ttttgaagaa atcgtcggca atgcctggat caggttgtgc aaaagcggga
     2221 cccatcaaaa acatggtgcc ggcaagcagg atcatatgca cagaatttct gaccatcttc
     2281 ctcaacctgt tcaatattca ggccaaattc aggaataaag ataatcaatg taacgaaagg
     2341 ggaaaatgcc ggggttcagg aatcgctgta ttccgggaaa taggatgatg attaagtctt
     2401 atttgactat gtattagtaa ctaagagtta cctgagccat tccgggaatg tcgatcttaa
     2461 attcaacacg acggttgagt tgacgtcctt cgggattgtc tttaccatcg gggtaggcat
     2521 tggcagctgc cggacgggat tcgccgtgcc atgcggattt gatccttgat tttgcgattc
     2581 ctttacttac cagaaaggtg taagttgaat tggatctgcg gctggacaag cccatattgt
     2641 aatcttctgt tccgatatta tcacaatgac catcgatcgc aatggttact tccggatttg
     2701 ctaccataaa ggcataaatg atatcgagaa tctcaacact gcggttgcgg aggaagaatt
     2761 tatcgaaatc gaagtaaatg ttttcaaatg caggaacttc cttggccagc ttttcaagaa
     2821 ataatctgtc aatgaattct gagtattcaa gtccggtttt tccgggttga atggtacgga
     2881 tataagaccc gtaaaggata ttgatatcgt cggcctttat tctgtaaata aatgccattt
     2941 cctggttcag acggttgagg tccttattga gatctgcttt tttcccgaat ttaaaattcc
     3001 ctttgtcgtt ggttttcgac aggtgagtga tttgtttgtt atcgtctaca aagtagattt
     3061 ccgtattggc taatcctgtt gtattacggc ttctgagatc tgtttgttcc agtctgcctt
     3121 ctaacgtaat ttctgcagta gcaccttctt tggtatcgcc gaaataactg attcttgcac
     3181 tggtttcgtc aacgagaatt ttatagggtt ccttgggatc aataaacatg aattggaaaa
     3241 atccgtgttc gtcagatgta gtactataaa tgatttcgtt tttggagttc accaaataaa
     3301 tgatcaaacc ttttccggga ataacggagt tgtgacggat gttcccactg atcatcaggt
     3361 aatgatcagg gttttgcaat tgatcaattc ccgattcact ttccggaagc ttgcggagtg
     3421 cataggtgga agtgatctcg ttattcaggt tgaagaatgc atttctgaat tttgattcct
     3481 gcgccattgc attctcgata ctgtcgcgaa cacgacgaat attgatctcc tgataaagag
     3541 gaaaaaactc acactgtttg ggaagtgtaa aggtttgttt gtgcacatcg ccggcttcaa
     3601 atccctccac ttcaacttcc atgtcgtaag tattgtttgg aggcagtaca attacatagc
     3661 gacctgaata gggattgctg gtataatttc cgacaatttt tccgctcgat ttttccttaa
     3721 ttgtaatcct tccgaaagca ggtagttgtc gttcgccagc cagaatagtg cctcttaatt
     3781 cggtattggg aatgtcttta catcctaact gaatacggta aatatccata tcaccatatc
     3841 cgccaactct cgatgatgca aaatatccaa atgtgttgtt gatgtcaaca tgaaaataaa
     3901 tatcatctcc ggcagagtta atcggaatcc ccatgttgat aggatctcca aatactccat
     3961 tactatgtgg acatttgaaa atatcgtaac caccaatgga gttgtgcccc ttcgaagaga
     4021 aataaaaggt ctgtaaatcg ctgcttaaaa acggagcatc ttcgtcttca ggtgtattga
     4081 tgtcttctcc taaattgatt gcaggtcccc atgtaccgtc ttcctgctga taggaaacat
     4141 ataaatcacg accaccgaaa ccaccttcac gtgtgcttac aataaacagg attttttcat
     4201 taggagtaat gtgtatcgag ggttcgtgtt gtttggtatt cacattcctg ttcatacgaa
     4261 cggggtctgt ccatacacca tttatttgtt ccgattgcca gatatcatta tcgcgataaa
     4321 taaacagttt ggtttcgtct gcattaaatc ctatggctgc atcgtgggtt ttggtattga
     4381 tcttgctgct gatataaata ccggcagaat caaacttggt tggaaatgca aattgcaggg
     4441 aatcggcttt cggactggcg taaatatctt cgaagtattt agaatcgtaa aataactgtc
     4501 cgcctgtact gccttctc
//
LOCUS       DAOMYG010000275         4501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6776_length_4501_cov_8.118309, whole genome shotgun sequence.
ACCESSION   DAOMYG010000275 DAOMYG010000000
VERSION     DAOMYG010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6776_length_4501_cov_8.118309"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PK610_09325"
     CDS             complement(<1..452)
                     /locus_tag="PK610_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRJ36178.1"
                     /translation="MAKNLVIVESPAKAKTIEGFLGKDYIVKSSFGHVRDLAKSGLSI
                     DIDNGFLPHYEVSEDKKKVITELKKLSKGAEVIWLATDEDREGEAISWHLKEALELPE
                     NRTRRITFNEITKNAILKAISKPRKIDKNLVDAQQARRVLDRLVGFELS"
     gene            660..2096
                     /gene="miaB"
                     /locus_tag="PK610_09330"
     CDS             660..2096
                     /gene="miaB"
                     /locus_tag="PK610_09330"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRJ36179.1"
                     /translation="MQREDSEMLEEGRQGEAVMVNPDPSKLKNGRKLLLESYGCQMNF
                     SDSEIVASILTGAGYTTTRDMEEADVVLMNTCAIRDNAEQKVRNRLQEFHARKRVKPG
                     LVVGILGCMAERLKASLLEEEKLVDIVVGPDAYRDLPNLIEQVEGGQKAVNVLLSREE
                     TYADINPIRLDGNGVSAFISIMRGCDNMCSFCVVPFTRGRERSRDPESIIHEANYLFQ
                     QGYREVTLLGQNVDSYKWNMNSKGEILDAAKPTLNFAGLLERVAQVSPLLRIRYSTSH
                     PKDMTDEVLEVMARHDNICKYIHLPVQSGNSEILTKMNRGYSREWYLDRIAAIKRIMP
                     DCAISTDVITGFCSETDAQHYDTIRLMEEVRFDFAYMFKYSERPKTLAERKFEDDIPE
                     EIKTKRLNEIIALQQKHSLESNQRAVGKVHRVLVEGTSKRSDQHLFGRNTQNAVVVFP
                     KEGYNKGEYVDVLVTECTAATLRGEAVK"
     gene            2110..3363
                     /locus_tag="PK610_09335"
     CDS             2110..3363
                     /locus_tag="PK610_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRJ36180.1"
                     /translation="MQNITSIKQRFGIIGNSTLLNRAIEIASQVAATDLSVLVTGESG
                     TGKEIMPQIIHNLSSRKHGPYIAVNCGAIPEGTIDSELFGHEKGSFTGAHEARKGYFE
                     VANTGTIFLDEVAELPLATQSRLLRVLESGEYMRVGSSKVMKTDVRIIAATNVNIPEA
                     IRKGKFREDLYYRLNTVPIFIPALRERKEDIILLFRKFASDFAEKYRMPAVRLDEEAT
                     QILLKFSWPGNIRQLKNITEQISVIEQEREIRSNELLQYLPKENTGLPAISGNLGAGG
                     DLNEREILYKIIFEMRSELNDLKSVVMELLQNRSPEISGNKNAGLLQRLYSGESEPVI
                     STGDSQIIRSNPINPMVHSSTPRYSDDIAEHVEVEESLSLEEKEKDLIKKALHKHRNK
                     RKYAAKELGISERTLYRKIKEYNIK"
     gene            3360..3863
                     /locus_tag="PK610_09340"
     CDS             3360..3863
                     /locus_tag="PK610_09340"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HRJ36181.1"
                     /translation="MRNFLSYFIIASVLFVSCKVRYSFTGGEIPSEAKTVSVSYFQNN
                     APLAGPNVPQQFTEALKDLFIAQTKLNLVKDNGDLRFEGAITGYDVRPVALQGNDAAA
                     LNRLTLSVNVRYINSFDDKKNFEQNFSRFVDFPSTTDIASNEESLIGEINKQLVQDIY
                     DRSFGNW"
     gene            3868..>4501
                     /locus_tag="PK610_09345"
     CDS             3868..>4501
                     /locus_tag="PK610_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36182.1"
                     /translation="MNSQTLYQLTQHPAAIAQDDVKLLSELSDRFPWCQPVQMLLSKG
                     LHNTKSIAYLDHLKRTSAITSDRSVLYHFILQQGILEKLEKLDKVIEEELRIEENNAP
                     ELTIAEEQTELQQHPDKKSATIIPISSNPYAELEGVDKDQAMVLVTKDILEDLIERKI
                     KDFVPTDTSTEILHQKTSEEQTIEEITEEISSPDIIDPDSSVEIKEKSIVE"
BASE COUNT         1422 a          882 c          970 g         1227 t
ORIGIN      
        1 gaaagttcaa acccgaccaa acgatctaaa acgcgacgtg cctgttgggc atccacgagg
       61 tttttatcga ttttccgcgg tttggagatc gcctttagaa ttgcgttttt ggtaatttca
      121 ttaaaggtta ttcttcgtgt ccggttctca ggtaattcaa gcgcttcctt taagtgccat
      181 gaaattgcct ctccctcccg gtcctcatcc gtagcaagcc agatcacttc tgctcccttg
      241 gatagttttt tcaactcggt gatcaccttc tttttatctt cagaaacctc ataatgagga
      301 agaaagccat tatcaatatc gattgataag ccactttttg caagatcgcg cacatgcccg
      361 aaacttgatt ttacgatgta gtccttacca aggaaaccct caatggtttt cgctttggcg
      421 ggggactcca caataaccag attctttgcc atgaatattt attgaatttg gtggacggaa
      481 cccgaaagcc gggtgccggg gggtgcaaat aaagcgaaaa ggaacctttg aaaaaaacgc
      541 cgggtctcag cattttttat aaacaaacgt taaaaaccca attttaaggg tctgtcattt
      601 tgacattaac ccctgtctta ctatctttgc attccttgaa aaatcaaggg gtttaattca
      661 tgcaaaggga agattcagaa atgttggaag agggtcgcca gggcgaggct gtcatggtaa
      721 atcccgaccc gtcgaagtta aaaaatggcc gaaaattgtt gctggagagc tacggatgcc
      781 agatgaattt ctcggattca gagatcgtag cctcgatttt aacgggcgcc gggtacacca
      841 caacacgaga tatggaggaa gccgatgtgg tattaatgaa tacatgtgct attcgtgata
      901 atgcggaaca aaaagtgcgg aatcgtttgc aggaatttca tgcccgtaaa cgtgtaaagc
      961 ccggacttgt agtcggtatt ctcgggtgta tggctgagcg gttaaaagcc agtttactgg
     1021 aagaggaaaa acttgttgat atagttgttg gcccagatgc ctatcgtgat ctgcccaatc
     1081 tcattgaaca ggtagaaggc gggcaaaaag cagtgaatgt acttttgtca agagaggaaa
     1141 cgtatgcaga tatcaatccc atacgcttag acggtaatgg agtgagtgct tttatcagca
     1201 ttatgagagg ttgtgacaat atgtgttcct tttgcgttgt tccgtttacc cgcggaaggg
     1261 agagaagtcg tgatccggaa tccattattc acgaagcaaa ttacctgttt cagcaaggtt
     1321 atcgtgaagt gacattatta ggacaaaatg ttgactctta taaatggaat atgaattcga
     1381 aaggagaaat ccttgatgca gccaaaccta cactcaattt cgccggactt cttgaaaggg
     1441 tagcacaagt ttcaccgcta ttaaggatcc gctacagcac ctcgcatcct aaagacatga
     1501 cagatgaggt tttggaagta atggcaaggc acgacaacat ttgcaaatac atccatcttc
     1561 ctgtgcaatc cggaaattcg gaaatcctaa caaaaatgaa ccggggatat tcgcgcgaat
     1621 ggtatctcga caggattgct gctataaaaa gaatcatgcc ggattgcgca atttctactg
     1681 atgttattac ggggttttgt tcggaaaccg atgcacaaca ttacgatacg atccgcttaa
     1741 tggaagaagt tcgttttgat tttgcgtaca tgtttaagta ttccgaacga cctaaaacac
     1801 ttgccgaaag aaagttcgaa gatgatattc cggaggagat aaaaaccaaa cggctaaatg
     1861 aaatcattgc gttacaacaa aagcattcac ttgaaagcaa tcagcgagca gttgggaaag
     1921 tacatcgtgt attggttgaa ggaacgtcaa aaagatcaga tcaacatttg ttcggcagaa
     1981 acacccaaaa tgcagttgtt gtttttccaa aagaaggata caataaaggc gagtatgttg
     2041 acgtccttgt tactgaatgc acggctgcaa cactgcgagg tgaggccgta aaataatttt
     2101 agaatgaata ttcaaaatat tacaagcata aaacagcgct ttggcattat tggtaattcc
     2161 acattgctga atcgtgccat tgaaattgca tcccaggttg cagcaaccga tctttccgtt
     2221 ttggttaccg gagaaagcgg aactggtaaa gaaatcatgc cgcagatcat tcataatctt
     2281 tcttcaagaa aacatggtcc ttatatcgct gtcaattgcg gagcgattcc cgaaggaacc
     2341 atcgattcgg aattatttgg tcatgaaaaa ggttcgttta ccggcgctca tgaagcacga
     2401 aaaggatatt ttgaagttgc aaataccggt acgattttcc tggatgaagt tgcagaatta
     2461 ccgcttgcca cgcaatcaag attactccgg gtactggaaa gcggagaata catgagggtt
     2521 ggttcatcta aagtgatgaa aaccgatgtt agaattattg cagctaccaa cgtaaacatt
     2581 cctgaagcta tacgaaaagg aaaattcagg gaagatcttt actaccgatt aaacacagtt
     2641 ccaattttca ttcctgcttt gcgcgaacgg aaagaggaca tcattttact atttagaaag
     2701 tttgcttctg attttgctga aaaataccgc atgcctgctg ttcgtttgga tgaagaggcg
     2761 actcaaattc tactaaaatt ttcctggccg ggtaatattc gtcagcttaa aaacattacc
     2821 gaacaaattt cagtaattga gcaggaacga gagattcgct cgaatgaact tttgcaatat
     2881 ttacccaaag aaaatacagg tcttcctgcg attagcggta accttggtgc cggtggagat
     2941 cttaatgagc gtgaaattct ttacaagatc atttttgaga tgcgttccga attaaatgat
     3001 ctgaaaagtg tggttatgga attgctccaa aatcggagtc ctgaaatttc aggtaataaa
     3061 aacgcaggat tgttacagcg attgtattcc ggagaaagcg agcctgtaat cagcacagga
     3121 gattcacaga ttataagatc taatccaatt aatccgatgg tacactcctc tactccacgc
     3181 tactcggatg atattgcaga acatgtggag gttgaagaat ccctttccct ggaagaaaag
     3241 gaaaaagacc tcataaaaaa agcacttcac aaacaccgca ataaaaggaa atacgcagcc
     3301 aaggaattgg gtatttctga aagaacgtta tatcgcaaaa taaaagagta taacatcaaa
     3361 tgagaaattt tctctcctat tttataattg ccagtgtact tttcgtatcc tgtaaagtcc
     3421 gctattcatt taccggaggt gaaattccat ctgaagcaaa gactgtttcg gtaagttatt
     3481 ttcaaaacaa tgcaccactt gcgggtccta acgtacctca gcaatttact gaggcgttaa
     3541 aagatttgtt tattgcacaa accaaactca acctggtaaa agacaatggt gatttacgat
     3601 ttgaaggtgc aattaccgga tatgatgttc gtccggtagc ccttcagggt aatgacgcag
     3661 cggcactaaa cagattgact ttatcagtta atgtgcgata cattaattca tttgatgaca
     3721 agaaaaactt tgagcagaat tttagtcgtt ttgtggattt cccaagcact accgatattg
     3781 cctctaatga agaatcattg atcggagaaa ttaacaaaca attggtacag gatatttacg
     3841 atcgctcatt cggaaactgg taaattcatg aattcacaaa cgctatatca gctcacccaa
     3901 catcctgcag ctattgcgca agatgatgtt aagctattat cggaattaag tgaccgtttc
     3961 ccctggtgtc agcctgtaca aatgttgctc tcaaaagggc ttcacaacac caaaagcatt
     4021 gcctacctcg atcatctaaa acgcacttct gcaattactt ctgatagaag tgttctctat
     4081 catttcattc ttcagcaagg gattcttgag aaattggaga aattggataa agtcattgaa
     4141 gaggaattgc gtattgagga aaacaatgcc cccgaattga ctattgcaga agagcaaact
     4201 gaattgcagc aacaccctga taaaaaatcc gcaaccatca ttcctatcag cagcaatcct
     4261 tatgcagaat tggaaggcgt tgataaggat caggccatgg tgcttgttac aaaagacata
     4321 cttgaggatc tgattgagcg aaaaataaaa gattttgtac cgacagatac gtcaactgaa
     4381 atattgcatc agaaaaccag cgaagaacaa acaattgaag agataactga agagatttct
     4441 tcaccggata ttatcgatcc tgattcttca gtcgaaatca aagaaaaatc gatcgttgaa
     4501 g
//
LOCUS       DAOMYG010000276         4439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6915_length_4439_cov_12.096943, whole genome shotgun sequence.
ACCESSION   DAOMYG010000276 DAOMYG010000000
VERSION     DAOMYG010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6915_length_4439_cov_12.096943"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..250
                     /locus_tag="PK610_09350"
     tRNA            178..250
                     /locus_tag="PK610_09350"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Pro,seq:ggg)
     gene            297..542
                     /locus_tag="PK610_09355"
     CDS             297..542
                     /locus_tag="PK610_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRJ36183.1"
                     /translation="MYFVYILYSAQHDKFYIGQTNDLEDRLKRHNSGQVDATKPYLPW
                     EMLIRIEKDTRSEAMILEKKLKNLSKERLRLFIEKYS"
     gene            complement(753..>4439)
                     /locus_tag="PK610_09360"
     CDS             complement(753..>4439)
                     /locus_tag="PK610_09360"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HRJ36184.1"
                     /translation="YAQLIVTSGGGGTAVQGALLGPGLTVSGPITINCNAASYGSFSN
                     GNTTNLGVTTGLLMTTGNATGAIGPNGTGSGSFCHGTSASDPDLLALDPQANEDVCII
                     TVTVIPQCSQLTIRFVFGSEEYPEWVSSGYNDAFGFFVSGPNPGGGNYTNFNVARLPN
                     NTIVSIDNVNATTNNAYYVNNTGGTSIQYDGFTTVLQPTINVVPCQPYTFKLAIADAG
                     DCTYDSGVFIDFLSCTNNPTVSTTPVSATCGANDGTATANVTGGIGPFTYSWNTIPVQ
                     TTQTATGLAPGTYTVTVTDNGIPCNTPTTQTVTVGSTGVLPSTAVSPTSQSICPGGNV
                     ALTASGASSYTWSPATGLSATTGTNVTATPTSTTTYTVTGTNACGSVTATTTITVHPV
                     PNVTATPSPTSICSGSSPSIALSSTTGSSFNWTVSQSGTSGASNGSGTSINQTLSATG
                     AVPGTATYTVTPTANGCNGTPITVSVTVNPVPTATASNTSPVCIGNPVSLNGPTITGA
                     TYSWTGPGGFNSTLEDPIIPSLGAGQAGTYTLIVTLNGCPSAPATTTVTITPGVNPIV
                     NPAGPLCEDATTITLSADIPGGTWSGTGITNTSTGTFDPSVAGPGQHVITYTTTGGCA
                     GFGTTTIQVTPANGTAPSGTQFNTASNGVGGTLPGGSNDLNWEVSTSLAGPFAPAIVM
                     STIPGSYFASPWPDAGWIAHNANGSHAGNVDYYYRILFDLPCYDVCGFSYADSGVYCL
                     TMDFFADNSVNEVYINGVAQSPTQPLLPVANQYANIGFNAAGGLTITMCDDWQPGTNE
                     LIIKVSSGAPFEGFLAQYSTSSLNQPNDPTISAPANDTVFCSNEPTVALTSASTGGTW
                     ASNCGTCLDANGNFSPSVAGPGTYTVTYTTNQLCPATDNITLIVNPFTAANISTAGPF
                     CQNDPAINLTAVNPGGTWSGTGITSASAGTFNPATAAPGTHTITYSFGGNCPSSQSIS
                     ITVNATPTANAGNDQILPCVPPLVSLNGAGSSAGSYNWTSTNGTIVSGATTTTPSVSS
                     AGTYTLTVTSAAGCTATDAVLVTNATGPNAIFSVTPATGIPPLTVATNNSSTGNSISN
                     FWTSGDGDTSLQTSPGFTYDNAGNYTLTLVVTDINGCTDTATALIIVEEEFELLIPNV
                     FSPNGDGSNDVFRVQSKGVSDLHGRIYDRWGVFMAEFNGVNGYWDGKKDGKNATEATY
                     FYLISVTKMNGDVVEYSGHVTLVR"
BASE COUNT         1215 a          903 c         1076 g         1245 t
ORIGIN      
        1 atcttgatta agaaaatttt cgggatgtag ctcagttggt agagtacacg tctggtcctg
       61 acgaaccgca ttaagcggtt gtcaggatgc tgatatccgc caatggcgga tcgtcagcag
      121 gggcaatagt tttttgaaat aaaaagagaa tatcttaatc ttgattaaga aaattttcgg
      181 gatgtagctc agttggtaga gtacacgtct ggggggcgtg tggtcgcacg ttcgagtcgt
      241 gtcatcccga caaaaggaga ggcaaagcct ctcctttttt gtttatatca cattgcatgt
      301 atttcgttta cattttatat tcagcacaac acgataagtt ttatatcggt caaaccaacg
      361 atctggaaga tcggttgaaa cgtcataatt caggtcaagt ggatgcgacc aaaccctatt
      421 tgccttggga aatgttaatt cgtattgaaa aagatacacg ttcggaagcg atgattcttg
      481 agaagaagtt gaagaattta tcaaaagaaa gattgcgctt atttattgaa aagtattcct
      541 gaaagacatt ttccaatcat caaataatga aaattagtgg gggtcctgag gatcagctaa
      601 cgcttctgtc aggataccat cgcaagctct ggcaggcgtg tggtcgtgct gaccgttagc
      661 gtcagcatca tcccgaccaa aaaaagaaag aggcatttgg cctctttctc tgtttatata
      721 cgaacaatgt gtgttttggt taaagaaaaa atttatctca caagcgtaac atgcccggag
      781 tattcaacaa catcgccatt catttttgta actgaaatca gataaaaata agtggcttcg
      841 gttgcgtttt taccgtcttt tttaccgtcc caatatccgt ttacaccgtt aaactctgcc
      901 ataaacacac cccatctgtc atagatgcga ccgtgtaaat cgcttacgcc tttcgattga
      961 accctgaata catcattgga gccatcgcca tttggtgaga atacattggg aattaggagt
     1021 tcaaattctt cttccacaat aattaaggct gttgcagtat ctgtacaacc attgatatct
     1081 gttaccacca gtgtcagtgt gtaattacca gcattgtcgt aagtgaaacc gggagaggtt
     1141 tgcaaacttg tatcaccatc accgcttgtc cagaaattac taatggaatt tcctgttgag
     1201 gaattgtttg tagcaaccgt taaaggtgga attccggtag ctggtgttac actgaaaatt
     1261 gcgttgggac ctgttgcgtt tgtaacaaga actgcatccg tagctgtaca acccgctgcg
     1321 ctggtaactg taagtgtata ggttcctgca ctacttacgc ttggtgttgt tgttgtagct
     1381 cccgaaacaa ttgttccatt ggtgcttgtc caattgtacg agccggcaga agatcctgcg
     1441 ccgtttagtg aaaccaatgg tggtacacac ggaagaatct gatcgtttcc tgcatttgca
     1501 gttggagtag cattcactgt aatggaaata gattgcgatg atgggcaatt ccctccaaag
     1561 gaatacgtaa tggtatgcgt gcccggagcg gcagttgccg gattaaatgt tcctgcgctt
     1621 gcactcgtta ttccggttcc cgaccatgtt ccgcccggat taactgcagt gagatttatt
     1681 gcaggatcgt tctggcaaaa tggtcctgca gttgaaatat tagctgctgt aaatggattt
     1741 acaattagcg taatattatc tgtagcagga caaagctgat tggtagtata ggttaccgta
     1801 tatgttccgg gaccagcaac tgaaggagaa aagtttccgt ttgcatccag acaggttccg
     1861 caatttgaag cccatgttcc tcctgtagaa gcagaagtta atgcaacagt aggttcgtta
     1921 gagcagaaca cagtatcatt ggctggtgca gaaatagttg gatcatttgg ttgattaagt
     1981 gagcttgtac tgtattgagc caggaatcct tcgaatggag caccggaaga aacctttatg
     2041 atcaactcat tggtacccgg ctgccagtcg tcgcacatag tgatggttaa tccacctgca
     2101 gcattgaatc cgatatttgc atactgattc gcaacaggaa gaagtggttg agtaggactt
     2161 tgtgcaacgc cattgatgta gacttcattt acagaattat ctgcgaaaaa atccatcgta
     2221 aggcagtaca ctcccgaatc ggcatagctg aaaccgcaaa catcgtaaca gggaagatcg
     2281 aataaaatgc gataatagta atcgacattt cctgcatgtg aaccgttagc attgtgagca
     2341 atccaacctg catccggcca aggactcgcg aaataggatc cgggaatggt tgacattaca
     2401 attgcaggag cgaaaggacc ggctaatgaa gtggaaactt cccagttcaa atcgttggaa
     2461 cctccgggta aagtacctcc aacaccattg ctggccgtgt taaattgtgt tccgctagga
     2521 gctgtcccgt ttgcgggggt aacctgaatt gtagttgttc caaaacctgc acatccaccg
     2581 gtggtggtat aggtaataac gtgctgaccc ggacctgcaa cggaaggatc aaaagtaccg
     2641 gtacttgtgt tggtaattcc ggtaccagac caagtaccac ctggaatatc tgcactcaat
     2701 gtgatagttg tagcatcttc acagagcgga cctgcagggt taacaatggg attgacaccg
     2761 ggagtaatgg taacagttgt ggttgcagga gcagaaggac aaccattgag tgttacgatt
     2821 aaagtgtacg tccctgcctg gcctgctccc aatgagggaa taattggatc ttcgagtgta
     2881 ctgttgaaac cacctggtcc tgtccagctg taagtagcgc cggtaattgt aggtccgttt
     2941 aaagaaacag ggttaccaat acacaccgga cttgtattgg aagctgttgc agttggaacc
     3001 ggattaacag ttactgaaac agtgatcgga gttccattgc aaccatttgc ggttggagtt
     3061 acagtgtagg ttgcagttcc gggaacagca cctgttgctg ataaagtttg attgattgaa
     3121 gtaccacttc cattgctggc accactggtt ccgctttgtg atacggtcca gttaaaacta
     3181 gatccggttg tagaacttag agcaattgaa ggagatgaac cactgcaaat acttgtcggt
     3241 gatggtgtag ccgtaacatt tggaacagga tgtacggtga tggttgtggt tgctgttact
     3301 gatccacaag cgttggtacc ggtaactgtg taggttgtgg tgcttgttgg agttgcagtt
     3361 acattggtgc ctgttgttgc gctcaatcct gttgcaggcg accaggtata actgcttgca
     3421 cccgatgctg tgagtgccac atttcctcca ggacaaatac tttgacttgt tggactgact
     3481 gctgttgaag gaagaactcc tgtacttcca acggttactg tttgtgtagt tggagtattg
     3541 caaggaattc cattatccgt tactgttact gtataggttc caggagcaag tcccgttgct
     3601 gtttgagtag tttgcactgg aatagtattc caggaatagg tgaatggtcc gatccctcct
     3661 gttacattcg ctgttgctgt accatcattg gcaccacaag ttgcagaaac gggagttgtt
     3721 gatacagtcg ggttatttgt acaggataga aaatcgatga atacgcctga gtcataagtg
     3781 cagtcacccg catcagctat tgcgagctta aaggtgtatg gctgacaagg aacaacgttt
     3841 atggttggct gtaacacagt ggtaaaacca tcgtactgaa ttgaagttcc tccagtatta
     3901 tttacatagt aagcattgtt ggtagttgca tttacattat caatggaaac aatagtatta
     3961 tttggtaatc gggcaacgtt gaaattcgta taatttccac ctccgggatt tggtccggat
     4021 acaaaaaatc caaatgcatc attgtaacct gagctaaccc attccggata ttcttcagat
     4081 ccgaatacaa accgaatggt aagctgagaa cattgaggaa taacggtgac tgtaataata
     4141 caaacatcct cgttcgcctg ggggtcgagt gctaatagat cagggtcact ggcagaggtg
     4201 ccatgacaga atgatcctga acccgttccg ttgggaccaa ttgcgccggt tgcatttccg
     4261 gtagtcatta aaagaccggt tgtaacgcca aggttggttg tattaccatt ggaaaaactc
     4321 ccataagaag ctgcgttgca attgatggta atcggaccgc ttactgttaa acccggacct
     4381 aacaatgcac cctgaacggc agttcctccg ccaccagagg taacgatcaa ttgtgcata
//
LOCUS       DAOMYG010000277         4436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6922_length_4436_cov_8.682721, whole genome shotgun sequence.
ACCESSION   DAOMYG010000277 DAOMYG010000000
VERSION     DAOMYG010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6922_length_4436_cov_8.682721"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2410
                     /locus_tag="PK610_09365"
     CDS             <1..2410
                     /locus_tag="PK610_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36185.1"
                     /translation="PIGPNSRLELRGNGSNYVEWDCNGFVAMQLAGDMLFSRGILEPV
                     NEQEEEVKGHFSIRVEDIHALVVAITLDPFKVKGLSGWTFSVEDAVVDWSSTSDDPDM
                     RFPPGFEGESGLWTGTYLRRVSIALPSELSPVNGEASRVVAHNLLIDDSGLSAWIQAE
                     NTIPDLSMSGWKFSVEEIALQLLYNDPVGGHLRGTIELPVMDSGQALAYEAMMQFNPE
                     NGECNYSFSASPQGEIAFSVLSAKAELFPTTRISMSRTNGQFLPEAFLNGRIRFENER
                     LTTAQLQIQDVHLIHTAPFLTGGQIAYTGNSQNHAANYPVSIDSIRIGFSNQAPFIEM
                     KLMMNLAESQNNGLSVSTFLRVHSRMQEQKLHYDRVSIGAVNIAANSQAFQLNGTIHF
                     REDDPIYGNGFFGAISLSIPDVLEPGFEASACFGRTDFRYFAIDAAVPVKIPIAASGI
                     VMNRLIGGISNRMQPQRQQIGQVTNLLYANTVASGMSQTYTPDQNAGLTFRAGASLAY
                     LPNEETFNGDAMLDIGFNSNGGLDFVRLVGNAYFMSSIQSRRTSPPPVYGSMVMEYSV
                     PAKSFDASIQAQLNVQQTITGNGICRIHFDPNTWSVCIGRPTQPVVVSIQPFASAQAY
                     FMAGDNLEPMSPPPSQVASLVQNGGLNQMRDVSALSQARGIAAGMRLTGGLYREYGWD
                     FFTVYGSFSFGAGFDMMLIDYGPTAHCSGSLAPVGVNGKIAEGQLFAWMQGGIGAKGE
                     IGNKEFDVVLINGSIAALLQGKVPRPSWVSGGVQVQYQILSLIQGQFQFDFELGQTCT
                     VVVP"
     gene            2407..4293
                     /locus_tag="PK610_09370"
     CDS             2407..4293
                     /locus_tag="PK610_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36186.1"
                     /translation="MKHSSISIFFLFWCTRLFAQDYVFLGERTESSIVFRWAPSNADD
                     VRALLLKGTFLFGLANGKEVFLNRNKVWDEQDNRWDSLSVKEERILFLRRFLFGEQGG
                     SELNQMYSGFALLLADTDPVFADALGLIYELKDRDFERYLLRDKSGKTLAELTNAYIR
                     PKHLPRPEIADAECKHGKVILKWIPQRTIFSSWEVQYSSNSSDWKPATLQSLVPFLDE
                     NSGDSSMYFSDSLRSDSRRYYRIRGKTPFGNFSEYSPLIALECFNDEIVFPVLDLAES
                     SIDEIKLQIHFPLSADRKKTYQMVLERSDQYSGNWEKLREVFVSDSMMVLKQSKRYAY
                     YRLALQLYNKQYVFTDPVLVTLKEDGPPAIPEGISGRIDTLGYVRLNWRKNTESDLRG
                     YRIYSRNSSGDEWVERTQEVNRDTFFVWKSTRQTIERSLEVAIAATDSSWNTSALSQG
                     VLLARPDHIPPVKPVWKEIRYEKDSVFLRFAFDDSEQTSLVVLSNDSLSEKLVKIHSH
                     VLSRKSAIKFWLRCSDSSGNSSVSDTIYLVAERSFDAKIIADLSKNEIVLKYDSGTDI
                     IYRAVLYRKRNSGEWKAMQTAERASGIVIDRELQMQSRYSYRLKITLHSGRSYWSDEV
                     VVNY"
BASE COUNT         1198 a          888 c         1073 g         1277 t
ORIGIN      
        1 accaattgga ccaaacagca ggcttgaatt gcggggtaac ggaagcaatt atgtggaatg
       61 ggattgcaac ggatttgtag ccatgcagct tgccggtgat atgctttttt cacgtggaat
      121 tctggagccg gttaacgaac aagaggaaga agtgaaggga catttttcga ttcgtgtcga
      181 agatatccat gcccttgttg tggccatcac actcgatccg tttaaagtga aaggattgtc
      241 cggatggact ttttctgttg aggatgctgt tgttgactgg agttcaacat ccgacgatcc
      301 tgatatgcgt tttcctcccg gatttgaagg ggaaagcggc ctatggaccg gaacctattt
      361 acgcagggta agtattgctc ttccgtctga actttcgcct gtaaacggag aagcttcacg
      421 tgttgttgcg cataatttac tcattgatga ttccggactt agcgcatgga tacaggcaga
      481 aaatacaatt ccggatttaa gcatgagcgg gtggaaattt tcagtggagg agatagcctt
      541 gcaattgttg tacaacgatc ctgttggcgg acatctgcgg ggcaccatcg aattacctgt
      601 gatggattcc gggcaagctt tggcttatga agcaatgatg cagtttaatc ccgaaaatgg
      661 agaatgcaat tattcattca gcgcatcacc gcaaggggaa atagcttttt cagtactctc
      721 cgcgaaagca gagttatttc ctacaacacg catttccatg tcgcgtacaa acggacagtt
      781 tctaccggag gcgtttttga acggtagaat tcgttttgaa aatgaacgat tgaccactgc
      841 gcaattgcag attcaggatg tccatttgat ccatacagcg ccatttctta cgggcggtca
      901 gattgcctat accggaaatt cccaaaacca tgcagcgaat tatccggtca gtatagattc
      961 cattcgcata ggtttttcca atcaggcacc ttttatagaa atgaaactga tgatgaatct
     1021 ggcggaatca caaaacaatg gtctttccgt ttcaactttt ttgcgtgttc attcccgtat
     1081 gcaagagcag aaattgcact atgaccgtgt ttcaatagga gcagtcaaca ttgccgcaaa
     1141 ctcccaggct tttcagctga atggaacaat tcatttcaga gaggatgatc ctatatacgg
     1201 caatggtttt tttggagcca tctcactgag tattccggat gtattggaac ccggatttga
     1261 agcgtcggca tgttttggca ggacggactt tcggtatttt gccattgatg cagcggttcc
     1321 cgtaaaaatt ccaattgctg catcaggaat cgtgatgaac cgattgatag gtggaatttc
     1381 aaacagaatg cagccacaac gacaacagat cggacaagtc accaatttgc tttatgcgaa
     1441 tactgttgca agtggaatgt cgcaaaccta tacacctgat caaaatgcag gactaacatt
     1501 tcgtgcagga gcatcattgg catatttacc caatgaagaa acattcaacg gtgatgcaat
     1561 gctggatatc gggtttaact ccaatggagg attggatttt gtgaggcttg ttggtaatgc
     1621 ctatttcatg agcagtattc aatcccgaag gacaagtcct ccccccgttt acggatctat
     1681 ggttatggag tacagtgttc ctgcaaaaag ttttgatgca agtattcagg cgcagttgaa
     1741 cgttcagcaa acaataaccg gaaacggaat ttgccgcatt cattttgatc cgaatacctg
     1801 gagtgtttgt atcggtcgac caacacagcc tgttgttgta agtatacagc cctttgcaag
     1861 tgcacaagcg tattttatgg cgggagacaa tctcgaaccc atgagtccac ctccatcgca
     1921 ggtcgcatca ctggtgcaaa acggcgggtt aaaccagatg cgcgatgttt cagcgttgtc
     1981 gcaagcgcgg ggaattgctg caggaatgcg cttaacagga ggtttgtaca gggagtatgg
     2041 ttgggatttt tttactgttt atggttcttt ttcatttgga gccggatttg acatgatgtt
     2101 gatcgattat ggtccgacgg ctcattgttc agggtcgctt gctcctgtgg gcgtaaacgg
     2161 aaaaattgca gagggacaac ttttcgcctg gatgcagggt ggaattgggg cgaagggcga
     2221 aattggaaac aaggaatttg atgtggtatt gataaacggc tctatcgctg ctcttttaca
     2281 agggaaagtg ccacggccat cctgggtctc cggtggagtg caggtgcagt accagattct
     2341 ttctttgatt cagggacagt ttcagtttga ttttgaatta ggacaaacgt gtacagttgt
     2401 tgttccatga aacatagtag tattagtata ttttttctgt tttggtgcac ccggttgttt
     2461 gcacaggatt atgtttttct gggagagcga acagaatcat caatagtttt ccgttgggca
     2521 ccttcaaatg cggatgatgt cagagcgtta ttattgaagg gtactttttt atttggcctt
     2581 gctaacggaa aggaagtctt tttaaaccga aacaaagtat gggacgaaca ggacaatcgg
     2641 tgggattccc tttccgttaa ggaggagcgg attttgtttt tacgcaggtt tttattcggt
     2701 gaacaaggcg gttctgaatt gaatcaaatg tattccggtt ttgcgctatt gctcgccgac
     2761 accgatcctg tttttgcaga tgcgctgggg ttaatttatg aattaaagga ccgagatttt
     2821 gagcgttatt tactcaggga taaatccggc aaaacgctgg ccgaattaac caatgcatat
     2881 attcgtccga aacacttacc cagacccgaa attgcggatg ccgaatgcaa acatggaaag
     2941 gtgatactga aatggattcc gcaacggact attttttcat cctgggaagt tcaatacagc
     3001 agcaattcct ccgattggaa gcctgccaca ttacaatctc ttgttccgtt tttagatgag
     3061 aattccggcg attcctcaat gtatttttcc gattcgctac gtagtgactc caggcggtat
     3121 taccgaatcc gtggaaaaac tccttttgga aatttcagcg aatattcacc tttaattgcg
     3181 ttggagtgtt tcaatgatga aattgttttc cctgttttgg atcttgctga atcatctatt
     3241 gatgagatca aactacaaat ccattttcca ttgagtgctg accgaaaaaa aacgtatcaa
     3301 atggttcttg aacgatctga tcaatattcc ggcaactggg aaaaactgcg tgaagtattc
     3361 gtttccgaca gtatgatggt tcttaaacag agtaaacgtt atgcctacta ccgactggcg
     3421 ttgcaacttt acaataaaca atatgtattt acggatccgg ttctcgtcac ccttaaagaa
     3481 gatggtccgc ctgcaatccc ggagggtatt tccggcagga tcgatacgct tggttatgta
     3541 cgactgaatt ggagaaaaaa cacagaaagt gatctgcgcg gataccggat ttattcgcgg
     3601 aattcttcgg gcgatgaatg ggtggagcga acacaagaag taaaccgcga tacttttttt
     3661 gtgtggaaaa gcacccggca aacaattgaa agaagcctgg aagtagctat agccgcaacc
     3721 gattcttcct ggaacacttc cgctttgtcg caaggagttt tgctggctcg acccgaccat
     3781 atccctccgg taaaaccggt ttggaaggaa atccgttacg aaaaggactc tgtgttttta
     3841 cgcttcgcct tcgatgattc ggaacaaaca agtttagttg ttctttcaaa cgatagtctt
     3901 tctgagaagc ttgtcaaaat acattctcat gttttatccc gaaaatcggc aataaaattt
     3961 tggctcagat gttctgattc ttcaggcaat agttcggtct cagatacgat ttaccttgtt
     4021 gcagagcgaa gttttgatgc gaagattatt gccgatctgt ctaagaatga aattgtgctg
     4081 aagtacgatt ccggaacgga tatcatatac agagctgtgc tataccgtaa aaggaattca
     4141 ggagaatgga aagcaatgca aactgcagaa cgcgcatcgg gaatagtcat cgaccgtgaa
     4201 ttgcaaatgc agagcaggta ttcgtatcga ttaaaaatca cgcttcattc cggacgaagt
     4261 tattggtcgg acgaagttgt ggttaattat taaaacaaaa aaaatccccg cgttggcagg
     4321 gattttttga tctgtgactt atagttaaga tcagatcttc tcaacgttta ccgcgattgg
     4381 acctttacgg ccttcctcaa tggtgaaacg gacacggtcg ttctgctgaa catcag
//
LOCUS       DAOMYG010000278         4404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_6985_length_4404_cov_10.508163, whole genome shotgun sequence.
ACCESSION   DAOMYG010000278 DAOMYG010000000
VERSION     DAOMYG010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6985_length_4404_cov_10.508163"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PK610_09375"
     CDS             <1..433
                     /locus_tag="PK610_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRJ36187.1"
                     /translation="ILELYLDLEKLRFGDGFSFSINVDSGINQREVFIPPMVLQPYVE
                     NAMKHGLLHKKGTKTLSIQFSLSNQLECTIIDNGVGRKRSAEIKTRKHQSFASDAVEK
                     RLELLKEYTRLNYSVAIEDLEVNGESTGTKVKIRFPITDDL"
     gene            530..1291
                     /locus_tag="PK610_09380"
     CDS             530..1291
                     /locus_tag="PK610_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ36188.1"
                     /translation="MQSLRAIIIDDEERARHTLKVLLQDYCPNVEILAEAGNVPDAVL
                     AINKHKPDLIFLDIEMPEYNGFELMGFFREIDFDIIFVTAYSEYALRAFEVSATDYLL
                     KPVEIDSLKNAIDKIHSRQSRVALKQRLDLLKDTVHGEEIRKIALPMSDGLLFVEVAD
                     LVLVEADGAYSYVHLRDGSKILVSKKLKFFEDILNGRMHFFRPHRSYLININYIKKYH
                     RGENNLVMDNAQSVAISRDRKTDFDSLLKEMRLSV"
     gene            1384..2877
                     /locus_tag="PK610_09385"
     CDS             1384..2877
                     /locus_tag="PK610_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ36189.1"
                     /translation="MKNLLLLTTFSLCIFSAKSQVTTDLVSRWNFTNGNLRDVQGNND
                     AIASGAVPTTDRFGNANMAYFFDGINDHMYIGSPSNLNLDIMDQITISFWARPDATTG
                     GLRAMVAKWAGASNEQYGVFQNTTQTTVAIRTTNNSGVNVNTAFTSGEWKHIVFSYDK
                     TNSNNHKVYINGALILNQNFAIPYANTSSSTSLSLGCQLNDLNGTPGTPDRFFEGALD
                     DISIYSRVLTQSEVDSLFDAEANQCSGFAVDVIATNHATNNDGSIVFNVLGGLAPYHY
                     SVNGSTVIQMNSSSVCAYSFEGGTSSFTAAAGTVFTGVHFASYGASQGTCTDLLYGNC
                     HADNSQSITEDSLIGRNSGSINTHNGIFGDPCFGTGKFYRARVSYAEAITLNALAPGM
                     YEISITDSLGCSASTTIVINSTNGLTEYHKSSFVIFPNPSSDEIRISGPSYNSAECNI
                     YSADGRMVYSGIVTPDKAISVAAFVSGVYIVQINDNNNIYFSRLIRQ"
     gene            2990..4060
                     /locus_tag="PK610_09390"
     CDS             2990..4060
                     /locus_tag="PK610_09390"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRJ36190.1"
                     /translation="MNSKWINTAAVLIVIIIGGFLLIYFESLFKPLVIGGLLSLILLP
                     VYRKLLQWRLPRFVAMTGTVLLTASLLTFLISLFSTEVKHIGQELPELLPGMERKLDD
                     LGNIIENYSGITHDDQVGYIKNALRDISGNKEQLFSQLFKTAGGILSLVLSLFYMMMF
                     LLYKDGIRRFFLMVAVRKQEQSNSIMNDIKVMMKNYFIGILTVMLIVGIVIAGGLSLL
                     GIKHGVFFGLLAALLSIVPYVGIISSGIICIFYSWAGSESFWIPVIVIAIYYSVQLLE
                     GNLLTPFITGGSAKINPLATLFGLLLGSALWGVTGMILAIPMLATTRIICDHVPEWKA
                     IAYLIGEDLAEEKSIVSKKKKN"
     gene            4071..>4404
                     /locus_tag="PK610_09395"
     CDS             4071..>4404
                     /locus_tag="PK610_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36191.1"
                     /translation="MRILSISLVITAMIVLFSCGQESQVSNSTSDSLTNEKKDYASLV
                     QCLASSTALEDPDKCLKSHPELLESLTETQTDSISYYLIEALNRVSNSLDISGDYELD
                     VAKESKKLE"
BASE COUNT         1292 a          928 c          874 g         1310 t
ORIGIN      
        1 tattctcgag ctgtacctcg atcttgaaaa actacggttt ggtgatggct tttctttctc
       61 tatcaatgtc gattccggaa ttaatcagag agaagttttc attcccccaa tggtattgca
      121 gccctatgta gaaaacgcaa tgaaacatgg cctgttgcac aaaaaaggaa caaaaacact
      181 ttccattcaa ttttcactca gtaatcagct tgaatgcaca ataattgata atggtgttgg
      241 aagaaagcgt tctgccgaaa ttaaaacccg gaaacaccaa tccttcgcga gcgatgctgt
      301 tgaaaaacga cttgaattac tgaaggaata tacacgtctt aactattcag ttgcaattga
      361 agaccttgag gtaaatgggg agtccacagg cacaaaagtt aagatccgtt ttccgatcac
      421 tgacgattta tgaactcaag ttgccgattc atgaatcaac tacagatctt gtcgtttctt
      481 cacattacat ttgcatcctc attcaattcc ccacaccaaa caaccaattg tgcaatcact
      541 cagggcaata ataattgatg acgaggaaag agcccggcat actttaaaag tattgctcca
      601 ggattattgt cccaacgtcg aaattcttgc ggaagccggt aatgtgcccg atgcagtatt
      661 ggcaatcaat aaacataaac ccgatcttat ctttctcgat atcgaaatgc ctgaatacaa
      721 cggattcgaa ttaatgggtt ttttcaggga aatagatttc gatatcatct ttgtcacagc
      781 atatagtgaa tacgcacttc gcgcatttga agtgtccgca accgactatc tgttaaagcc
      841 tgtagaaatt gattccctca aaaatgcaat agacaagatt catagccgac aatcacgcgt
      901 tgcactgaag cagcggttgg atttgctaaa agatacggtt catggtgaag agatcagaaa
      961 aatagcacta ccgatgtcag acggactctt gtttgtggaa gtggcagatc tggttctggt
     1021 agaagctgac ggtgcctatt cgtatgtgca tttgcgcgac ggatcaaaaa ttcttgtgag
     1081 caaaaaatta aaattcttcg aagatattct aaatggaaga atgcattttt ttcgtccaca
     1141 tcgttcctac cttattaata tcaattacat caaaaaatac catcggggag aaaacaacct
     1201 tgtaatggat aacgcacaat cggtagcgat ttcccgcgac agaaaaactg atttcgactc
     1261 cttacttaaa gaaatgcgat tatctgtgtg aacatttcgg ttcatgaact taaacctgca
     1321 attcatgaac tcatctataa tctgttgggc ttttcgctct tgatttgcat aaaatcaaag
     1381 cttatgaaaa accttttact actgaccaca ttttccttat gcatttttag tgctaaatct
     1441 caggtaacaa cagatttggt ttcacgctgg aactttacca atggtaatct tcgtgatgtg
     1501 caagggaaca atgatgccat tgcttcggga gctgttccta caaccgatcg tttcggaaat
     1561 gccaacatgg cctatttttt cgatggcatc aatgaccaca tgtacatcgg gtcgccttcc
     1621 aatctcaatc tcgacatcat ggaccaaatc acgatttcat tttgggctcg tcctgatgca
     1681 acaacaggtg gactccgggc aatggtcgca aaatgggcgg gtgcatcaaa cgaacaatac
     1741 ggagtgtttc agaacacaac acaaacaaca gttgcaatcc gcaccaccaa taattccgga
     1801 gtaaatgtca acactgcctt tacctccggc gaatggaaac atatcgtttt cagttatgat
     1861 aaaaccaata gcaacaacca taaagtatat ataaacggag cacttattct caaccagaat
     1921 tttgctattc cctatgccaa taccagcagc agcaccagtc tctcgcttgg ttgtcaatta
     1981 aatgatctca acggtactcc gggaactccc gatcgttttt ttgaaggcgc tttggatgat
     2041 attagcattt atagcagggt cctgactcaa tcagaagttg actccctatt cgatgcagaa
     2101 gccaaccaat gttcgggttt tgcagtagac gttatcgcca ccaatcacgc aacaaacaac
     2161 gatggaagta ttgtgtttaa tgtgcttgga ggtttagctc cttaccacta ttccgtaaac
     2221 ggatcaacgg taattcaaat gaattcaagc tctgtctgtg cgtattcctt cgaaggagga
     2281 acgtccagct ttactgcggc tgcgggaact gtatttaccg gtgtacactt tgccagttac
     2341 ggggcctcac agggaacctg tactgatctg ctttatggaa attgtcatgc cgataattcc
     2401 caaagtataa cagaagattc gctcattgga cgaaattccg gctccatcaa tacccataac
     2461 ggaattttcg gcgatccctg ctttgggaca ggcaaattct acagagcaag ggtttcctac
     2521 gctgaagcca ttacccttaa cgcgcttgct ccgggtatgt atgaaattag cattacggat
     2581 tctctgggtt gctccgcttc aacaacgatt gtaatcaatt caaccaatgg gcttacagaa
     2641 taccataaat catccttcgt gattttccct aacccctcca gtgatgaaat cagaatttca
     2701 ggcccttcgt ataattctgc cgaatgcaat atctatagtg ctgacggaag aatggtttat
     2761 tccggaattg taacgcctga taaagcgatt tctgttgctg cgtttgtttc cggtgtttac
     2821 attgtacaga tcaacgataa caacaacatt tactttagtc gtctgatacg ccagtaattt
     2881 caatacccct tacagaaaac catcgtctca caaaagatga tggttttttg ctttcaacca
     2941 aatcctaatt ctttatctgc aatttgtggt aaaaatccta ttttgcagta tgaattcgaa
     3001 atggatcaat accgccgccg tactcattgt tatcattatt ggaggattct tactgattta
     3061 ttttgagtcg cttttcaagc ctcttgtcat tggcggactc ctatcgctca tcctactacc
     3121 ggtataccga aaactactgc aatggcgact cccgcgtttt gtggcaatga ctgggacagt
     3181 attgcttact gcatctcttt tgacctttct gattagttta ttctcaactg aagtaaaaca
     3241 tattggacag gaattgccgg aactacttcc cggaatggaa agaaagctgg atgatctggg
     3301 taatattatt gagaattata gcggaataac gcatgatgac caggtaggct atattaagaa
     3361 tgccctgcgc gacataagcg gaaataaaga acaattgttt tcgcaacttt ttaaaactgc
     3421 aggtggaatt ttaagtctgg tcttgtctct gttttacatg atgatgtttt tgctctacaa
     3481 agacgggatt cgacgctttt tcttaatggt tgcagttcga aaacaggaac aaagcaacag
     3541 tattatgaat gatattaaag taatgatgaa aaattacttt attggaattc ttactgtgat
     3601 gttgattgtt ggaatagtga ttgcaggcgg actatcactt ctgggtataa aacacggtgt
     3661 tttctttgga ttgttagcgg cactgttaag tattgttcct tatgtgggaa ttattagttc
     3721 cggtatcatt tgcatttttt attcatgggc cggaagtgaa agtttttgga taccagtaat
     3781 tgtaattgca atttactaca gcgttcaact attggaaggc aatttgctta cgccatttat
     3841 aacgggtggt agcgcaaaaa ttaatccgct tgccacatta tttggtttat tactgggttc
     3901 cgcactatgg ggtgttacgg ggatgatcct agctattccc atgctggcaa ctacacgtat
     3961 tatttgcgat catgtacccg aatggaaggc gattgcttac ctgatcggtg aagatcttgc
     4021 tgaagaaaaa tcgatcgttt caaaaaagaa aaaaaactaa ctcataccca atgcgcatcc
     4081 tttccatttc acttgtcatt acagcaatga ttgtactctt ctcgtgcgga caagagtcac
     4141 aggtatcgaa tagcacttct gattcactta ccaatgaaaa gaaagactac gcgagtctcg
     4201 ttcaatgtct tgcttcttca actgcactgg aagatccgga caagtgttta aagtcgcacc
     4261 cagagttgct tgaatcactc actgaaacac aaactgattc catttcctat tacctcattg
     4321 aggccttgaa tcgtgtttcc aattcattag atatttccgg cgattatgaa cttgatgtgg
     4381 caaaagaatc gaaaaaactc gagc
//
LOCUS       DAOMYG010000279         4380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_7046_length_4380_cov_8.361156, whole genome shotgun sequence.
ACCESSION   DAOMYG010000279 DAOMYG010000000
VERSION     DAOMYG010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7046_length_4380_cov_8.361156"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PK610_09400"
     CDS             complement(<1..927)
                     /locus_tag="PK610_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRJ36192.1"
                     /translation="MELIPVALYIFFLSFILLYSIAELGLLFNFLFRLRKFRSLNPEP
                     PIKEEDLPKVTIQLPVYNEVYVVERLIDAVAQLDYPVHLLEIQVLDDSTDESAELAAK
                     RILYWKENGISIHHIRRNERTGFKAGALDYGIRLASGAFIAIFDADFIPPAYFLRRTI
                     PFFSNEKVGVVQTRWGHLNKNYSLLTRLQAFGLDAHFSIEQGGRNSAGHFINFNGTAG
                     VWSKSCIEDSGGWSADTLTEDLDLSYRAQLLGWKFIYLEEIVSPAELPAEMNALKAQQ
                     YRWSKGAAECARKNLAVPGGDEHPAGGAERRRH"
     gene            complement(927..1610)
                     /locus_tag="PK610_09405"
     CDS             complement(927..1610)
                     /locus_tag="PK610_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRJ36193.1"
                     /translation="MIIDVVIPAFNEEQSLPLVLGAIPRDLIRQVIVADNNSTDRTAE
                     VATAHGAHVVKATTQGYGAACLSGIAYSRACQPAPDILVFLDADYSDHPEELPLLTEP
                     IIANESDLVIGSRVKALRERGAMLPQQVFGNRLATFLIRVLFRKKFYDLGPFRAIRFE
                     SLEKLKMRDTNYGWTVEMQIKAIKHNLRWKEIPVHYRKRKGVSKISGTVKGTFLAGYK
                     IITTIIKYR"
     gene            1703..2800
                     /locus_tag="PK610_09410"
     CDS             1703..2800
                     /locus_tag="PK610_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRJ36194.1"
                     /translation="MAAPLKEIFNEAEIRRLAKIARQADGKFSESQFIANLINRDWKH
                     LELKQRMRRVAEELGQNLKGDFAKQSVALRKIAPQFSGLFGMSFPDYVEVYGKHDPEL
                     SLELLEFLTPFSSSEFAIRPFLLNEPDLTIRTMMKWSKSKNHHVRRLASEGSRPRLPW
                     AMAIPFLKKDPYPILPILYNLRSDKEDYVYRSVANNLNDISKDHPAITLRICKEWKDG
                     PEETQWVIKHALRTLLKKGDPAALKLQGHHHAAPAEIHQLRISANELIIGTEISFSCE
                     LKNPSSKPHLYRVEYGIDYVKSNGKTSQKVFQWKKINLRPGESVKLNSKQSLADMSTR
                     KHYPGVHKLHVLINGKSLNNKVFKLINKGKS"
     gene            2862..3314
                     /locus_tag="PK610_09415"
     CDS             2862..3314
                     /locus_tag="PK610_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRJ36195.1"
                     /translation="MKPEETIDFHIRWAWHGISRLYNGQAEKVNLTLSTGSVLLNIDQ
                     RSGTPSTKLGPKMGMEPRSLTRTLKSMEEEGLIMRKNDKNDGRMVRIHLTEMGKKKRE
                     ISKQAVIRLNEYLQERIPAGKLKTFFEVIDQINHVLEEETPDHLFGKK"
     gene            3331..>4380
                     /locus_tag="PK610_09420"
     CDS             3331..>4380
                     /locus_tag="PK610_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008991380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRJ36196.1"
                     /translation="MSHIRKVAVLGSGIMGSRIACHFANIGCQVLLLDIVPKELTEEE
                     KAKGLTAEHPAVRNRIVNLALQNTLKSNPSAIYSKKFISRITTGNFDDDMKKIADCDW
                     IIEAVVERLDIKHAVFSNVEQYRKPGSIISTNTSGIPIHMMLEGRSEDFKKHFCGTHF
                     FNPPRYLKLLEIIPTPSTDPKVIDFLMEFGSRNLGKTTVLCKDTPAFIANRIGVYGIM
                     ALFHLVEEMGLSVEEVDKLTGPVLGRPKSATFRTCDVVGLDTLVHVANGLQQNCPKDE
                     ANALFKLPAYVAKMNENKWLGDKTRQGFYKKTKKEDGSSEILALDLKTLEYIPQKKVK
                     FATLEGAKQIEELNKR"
BASE COUNT         1346 a          977 c          901 g         1156 t
ORIGIN      
        1 atgccgcctt cgttctgcgc cgccagcggg atgctcgtcg ccacctggta ctgccaggtt
       61 tttccgtgca cattcggcag cacccttgct ccatcgatat tgttgcgctt ttaaggcatt
      121 catctctgct ggcaattctg ccggtgaaac aatttcttcg aggtaaataa atttccagcc
      181 cagcaattgt gcccggtagc tgagatccaa atcttcggta agcgtatccg ccgaccaacc
      241 tccggaatct tcaatgcagg atttgctcca tacaccggca gttccgttaa aattgataaa
      301 atgtcctgca ctgtttcttc ctccctgttc aatactaaaa tgagcatcga gtccgaaagc
      361 ctgaagacgg gttaataagg aatagttttt atttagatgt ccccaccggg tttgtaccac
      421 tcccactttt tcattggaga aaaaaggaat cgttcgacgc agaaaataag ccgggggaat
      481 gaaatcagca tcaaaaatag caatgaatgc accggaggct aatctaattc cataatccag
      541 tgcacccgct ttaaatcctg tacgttcatt tctccgaata tgatgaatcg atattccatt
      601 ttccttccaa tacaaaatcc gtttcgctgc cagttctgca gattcatcgg ttgaatcatc
      661 caaaacctgg atctccaata aatgcacagg ataatcgagt tgcgccaccg catcaatcag
      721 gcgttctaca acataaactt cattatagac aggtaattga attgttactt tgggtaaatc
      781 ttcttctttg atcggaggct cgggattgag cgaacggaat tttcggagtc gaaacaaaaa
      841 attaaaaagt aatccaagtt ctgcaatgct atagagcaaa ataaaactca aaaaaaagat
      901 atataaagcc acaggaatca gttccatcag cggtatttaa taattgttgt aataatctta
      961 tatcctgcaa gaaaagtacc ttttaccgtt cccgaaattt tggatacgcc cttgcgtttt
     1021 ctatagtgaa caggtatttc cttccaacgt agattgtgtt ttatggcctt gatctgcatt
     1081 tctactgtcc acccgtaatt tgtatcgcgc attttcagtt tttcaagcga ttcaaagcgg
     1141 atggctctga atggtcccag atcataaaac ttctttcgga acaaaaccct gattaaaaat
     1201 gttgcaagtc tgttaccgaa tacctgctgc ggtagcattg ctcctctctc gcggagcgcc
     1261 tttacacgtg atccgattac aaggtcagat tcattggcga taattggttc agtcagcaaa
     1321 ggaagttctt cagggtgatc ggaataatcg gcatccagaa aaaccagaat atcaggtgcg
     1381 ggctgacatg ctcgcgaata agcaatacca ctcaggcagg cagctccata accttgtgtt
     1441 gttgctttca ctacatgggc tccatgtgcg gttgctactt cggcggttct gtcggtagaa
     1501 ttgttatccg ccacaatcac ctgccgaata agatcacggg gaatcgctcc cagcacaagc
     1561 ggtaagctct gctcttcgtt aaaggccgga atgactacat caatgatcat ggataccaac
     1621 aaaactaact tttttacgat tgctaaaccg ttgcatctta aatcgtttgc aaaaacctgc
     1681 atttttcatt tatttttaga ccatggcagc tcccctaaaa gaaatattta acgaagcgga
     1741 aatccgtcga ctggcaaaaa ttgcccgaca ggctgatggg aaattcagtg aatcccaatt
     1801 catcgctaac ctgatcaacc gcgactggaa acatctcgag ttaaaacaac gaatgcgcag
     1861 ggttgctgag gaattaggtc aaaacctgaa gggtgatttc gcaaaacagt cagttgcact
     1921 tcgaaaaatt gcaccgcagt tttccggact cttcggtatg agctttccgg attacgtgga
     1981 ggtgtatgga aaacatgatc cggaattatc cttggaactg ctcgaatttc tcacaccctt
     2041 ttcatcttct gaattcgcta ttcgtccttt tcttctcaat gaaccggatc tcactatccg
     2101 aacaatgatg aaatggagca aatcaaagaa tcatcatgtc cgccgtcttg ccagtgaagg
     2161 ttcccgtccg cgattaccct gggcgatggc aattcccttt ctgaaaaaag atccgtatcc
     2221 catcctgcct attctttata acctgagaag cgataaagaa gactatgttt atagaagtgt
     2281 tgcgaataat ctgaatgata tcagtaagga tcatccggca attacactgc ggatttgcaa
     2341 agaatggaaa gatggtcctg aagaaacaca atgggttatt aagcatgcat tgcgaaccct
     2401 tctcaaaaag ggagatcctg ctgcattaaa gttgcaagga catcatcacg ctgctccggc
     2461 ggaaattcat caattgcgta tctcggcaaa tgaactgatc attggaacgg aaatttcctt
     2521 ttcctgtgaa ctgaaaaacc catcttcaaa accgcactta tacagagttg aatacggaat
     2581 agattatgtt aaatccaatg ggaagacttc gcaaaaagtg tttcaatgga aaaaaatcaa
     2641 tcttcgtccc ggagagtcgg tcaaactcaa ctccaaacaa tccctggccg atatgagtac
     2701 ccggaagcac taccccggcg tacacaaact tcacgtactg atcaatggca aatcactgaa
     2761 taacaaggta tttaaactga tcaacaaggg gaaatcatga tctttatcaa gttttgattt
     2821 tctgtatgca tgcataataa atttagttat atttgaccat catgaaacct gaagaaacga
     2881 tcgattttca catccgctgg gcctggcacg ggatctcgcg tttgtacaat ggtcaggctg
     2941 aaaaagtcaa cctcacttta tccacaggct ctgtcctgct taacatcgat cagcgcagcg
     3001 gaactccatc cacgaaatta ggtccgaaaa tgggaatgga acccagaagc ctaacccgca
     3061 cgctgaaatc catggaagaa gaaggattga ttatgcgaaa gaacgataag aatgacggaa
     3121 gaatggttcg cattcatctt acagaaatgg gcaagaaaaa gagagaaatc tccaagcaag
     3181 ctgttatccg tttgaatgaa taccttcagg aaagaattcc cgcaggtaaa ctgaaaacat
     3241 tttttgaagt gatcgatcag atcaatcatg tattggaaga agaaacaccg gatcaccttt
     3301 tcggtaaaaa ataatgtaaa caatcatcac atgtcacaca ttcgtaaagt tgcagtactc
     3361 ggttcaggaa ttatgggatc acgtattgcc tgtcatttcg ctaacattgg ttgtcaggtt
     3421 ttactacttg atatcgtacc aaaggaactc accgaagaag agaaagcaaa aggacttacc
     3481 gcagaacatc ctgctgttcg aaaccgcatt gtaaatcttg ctttgcaaaa cacacttaag
     3541 tcgaaccctt ctgctattta cagcaagaaa ttcatcagcc ggattaccac cggtaatttc
     3601 gatgatgaca tgaaaaaaat tgcggactgc gactggatca tagaagctgt tgttgagcgt
     3661 ctcgatatca agcatgccgt tttttccaat gtggaacaat accgcaaacc gggtagtatt
     3721 atttcgacca acacttccgg tattcccatc cacatgatgt tggaaggacg aagtgaagat
     3781 tttaaaaaac atttttgcgg tacgcacttt tttaacccac cgcgctatct caaattactc
     3841 gaaatcatac ctactccatc aaccgatccg aaggtgattg acttcctaat ggaatttggc
     3901 agcaggaatc ttggtaaaac aacagtactc tgtaaagaca ctccagcctt tatcgcaaac
     3961 cgaatcggag tgtatggcat tatggcacta tttcacctgg tggaagaaat gggtctgagc
     4021 gttgaagaag tagataagct gaccggtccc gttctcggac gaccaaaatc tgcaacgttc
     4081 cggacatgcg atgtggttgg actcgataca ttggtgcatg tggcaaacgg attacaacaa
     4141 aattgcccga aggacgaagc aaatgccttg ttcaagcttc cggcctatgt tgcaaaaatg
     4201 aatgagaaca aatggctagg agataaaacg cgacaaggtt tttataaaaa aaccaaaaaa
     4261 gaggacggca gcagtgaaat acttgcgctt gatctgaaaa cactggaata cattccccag
     4321 aaaaaagtaa aatttgcaac gcttgaaggt gcaaagcaaa ttgaggaatt gaacaaacgt
//
LOCUS       DAOMYG010000280         4358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_7085_length_4358_cov_9.233790, whole genome shotgun sequence.
ACCESSION   DAOMYG010000280 DAOMYG010000000
VERSION     DAOMYG010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7085_length_4358_cov_9.233790"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PK610_09425"
     CDS             <1..240
                     /locus_tag="PK610_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36197.1"
                     /translation="HPGLFQGCSVYVGSVVQVPDPCRKNNSILTASPRQIVFLGGLIP
                     KPILLGSDSQLFPVRELFPFTFFVSFWRRDGFSPN"
     gene            254..>4358
                     /locus_tag="PK610_09430"
     CDS             254..>4358
                     /locus_tag="PK610_09430"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRJ36198.1"
                     /translation="MQEPLQDTTTDIALIDSLNSEAWKINRTDPRRGVELSKQAFELA
                     EANEYLKGKAYALSNWGACQVWLGNYEESLEHSFEALRLLLELNDKQQMCSVQYSVFC
                     VFYFLNDYDNALKHILEGYRLAEQTDDRIGKANSLNGIGTVYYTTGDNEKAVEHLKEG
                     FAIAKELGEEQIQAKILDGLGSAYYNLGDFDNALRVKFECLEIAQRLGIKQVHSYALD
                     GIGKAYSGLGKFEESIGYFEKSLSLRKEMGFRAGEAETLLHLGETYFQLQKYESALCY
                     LTEALELATEVDAKETIYRTHKVLSEFFELQGDFVRQIDHLKKYYQLKEEHFSQRQSQ
                     KQRSLEMQFRMERMEKEKELLENKNKELETVFSDVVLLSEIGRRITASLSVEDINQII
                     YESLSSMMDAPSFGMGIYNDVKNMIQFPGYIEDGKILYSEYDVNDGNRLAPVCFSQEK
                     EILIGDLEKELQLHVPVKMAPVAGRHVTSLIYLPVKVNQKKVGVITVQSYNKNAYTEY
                     HLNLLRNLAVYAGIALENAKLYEGLEEMVQERSAEVIRQKEEIERSYYNTQLLSEIGQ
                     QITSTLNIAVIFNELYQNVNKLMPAECFGVRIYKPEEHVIEYKYEVERGVVDTEGFSV
                     SMDNIDNYSVWCVRNREVVFLNDNQNEYHKYTSKIVVPTGEMPHSLIFYPMIVGDKVL
                     GVITVQSFEKFAYKPYHIDILKTLGTYTAIALENANLYENLEVKVAERTLEVVAQKEE
                     IEKNHRDTELLSAIGQEITSSLSVEAINQQVFEKVNTMMDAPSFGIGLYDAAKDAIVF
                     PGYIEEGLEEEVIYTSDDQDRLAWLCFNQEREIMIGDYDAELTNYVARRKAAVAGRLP
                     NSVIYIPLKTTNKKIGVLTAQSFSKNAYTEYHLNLLRNLSVYVAIALDNAGLYEGLED
                     KVEQRTAEIRKAYDNTRLLAQIQQDIISSLSVETIISKVYENVNTLMDATSFGIGIFD
                     EESRHIIMPGFIESGVRMEEFSYSVDDPNRLAAWCFNNQKEIFINNYYEEYSKYITGI
                     KKAISGKDSTSIIYLPLMANGKQIGVITVQSYELNAYTEYHLDLLRSLAASAAIAIDN
                     ARLYENLEYKVRERTAEVVKQKEVIEEKNKHITDSIVYAKRIQEAILPPEDVFKLHLR
                     NSFVLYKPKDIVSGDFYWIERKENKILFAVVDCTGHGVPGAFMSIIGYNGLNQIVNEY
                     NITQPAEILNNLNRIISATLKQRAEESKIRDGMDLSVCSIDLENHKLEYAGANNPIFI
                     VRNNEVIEVKADKQPIGNFVGEEDFRFTNKEIDLLPNDRLYVFSDGYADQFGGPRGKK
                     LKYSTFRDLLIENCDLPMEEQKRVLDQMFETWRGDLEQIDDVCVIGVG"
BASE COUNT         1300 a          895 c         1012 g         1151 t
ORIGIN      
        1 cacccaggtc tttttcaagg ttgctcagta tatgttggta gcgttgttca agttccagat
       61 ccatgccgca aaaataattc tattttaaca gcctcacctc gacaaattgt tttccttggc
      121 ggattgatac ccaaacctat ccttttaggc tccgattcgc aactttttcc cgttcgagag
      181 ctttttcctt ttactttctt cgtttctttt tggagaagag acggattttc accaaattga
      241 gagtcccaaa attatgcagg agccacttca ggataccact accgacattg ctttgattga
      301 ttcgctcaat tcagaagcat ggaagatcaa tcgcaccgat cctcgtcgtg gtgttgagtt
      361 gagcaagcag gcttttgaac tggcagaagc aaatgaatac ctcaagggaa aagcctatgc
      421 actttccaat tggggtgctt gtcaggtttg gctggggaac tatgaagaat cgctcgaaca
      481 ttcgtttgaa gctttgcgct tactgctgga gttgaatgac aaacagcaga tgtgttcggt
      541 gcagtattct gttttttgtg ttttttattt tctcaacgat tacgataatg cactaaagca
      601 tattctggaa ggttatcgtc tggccgaaca aaccgacgac agaataggta aagctaattc
      661 gctgaatggt atcggaacag tttattacac caccggcgat aatgaaaagg cagttgaaca
      721 tctcaaagaa ggatttgcaa ttgcaaagga actgggcgaa gaacagattc aggcaaaaat
      781 tctggatgga ttaggaagtg cctattacaa tcttggtgat tttgataatg cattgcgggt
      841 aaaattcgaa tgccttgaaa ttgcacagcg cttaggtatc aagcaggttc attcctatgc
      901 actcgatgga attggcaaag cctattcggg attgggaaaa ttcgaggagt cgatcggcta
      961 tttcgaaaag agtttgtctt tgcgtaaaga aatgggattc cgcgcaggtg aggcggaaac
     1021 gcttttgcat ttgggagaga cctatttcca attgcaaaaa tacgaaagcg ctctttgtta
     1081 ccttaccgaa gcacttgagc tcgctaccga ggtggatgcg aaggagacga tctacagaac
     1141 gcataaagtt ttatctgaat ttttcgaatt gcagggtgat tttgttcgcc agatcgatca
     1201 cctcaaaaaa tactaccagc taaaagaaga acatttttct cagcgtcagt cgcaaaaaca
     1261 acgttcgcta gaaatgcagt tccgtatgga gcgcatggag aaggaaaagg aattgctgga
     1321 gaacaaaaac aaagaactgg aaaccgtttt ttcggatgtg gtattgttaa gcgagatcgg
     1381 aagaagaatt actgcgtcgc tttctgttga ggatatcaat cagatcattt acgaatcgct
     1441 cagcagcatg atggatgctc cttcctttgg aatgggtatc tacaatgatg tgaaaaacat
     1501 gatccagttt ccgggataca ttgaagacgg aaaaatactg tattccgaat acgatgttaa
     1561 cgatggcaat cgccttgctc cggtttgttt ttcacaggag aaggaaattc ttatcggaga
     1621 tctcgaaaaa gaactgcaac tgcatgtgcc ggtaaaaatg gctcccgttg caggacgaca
     1681 tgttacatct ctgatctatc ttccggtaaa agtcaaccaa aagaaagtcg gcgtaattac
     1741 cgtacagagt tacaataaga atgcttatac cgaatatcat ctgaatcttt tgcggaatct
     1801 tgctgtttat gcaggtatcg cattggagaa tgccaaattg tatgaaggtc ttgaagaaat
     1861 ggttcaggaa cgttctgctg aggtcattcg ccagaaggaa gaaattgaac gttcgtatta
     1921 caacacccaa ttgctttcgg agattggtca gcagatcaca tctacgctca acattgctgt
     1981 tatttttaat gagctctacc agaatgtaaa caaactcatg cctgccgaat gtttcggggt
     2041 gaggatttac aaaccggagg aacacgttat tgaatacaaa tacgaagtag aacgtggagt
     2101 agtggatacg gaaggatttt ccgtttcgat ggataacatc gataactact cagtatggtg
     2161 tgtgcgtaac cgggaagttg tgtttttgaa cgacaaccag aatgaatacc acaaatacac
     2221 cagcaagatt gttgtcccca ccggcgagat gccgcactcc ctgatctttt atccgatgat
     2281 agtgggcgat aaagtgttgg gtgtaattac cgtacagagc ttcgaaaagt ttgcctataa
     2341 accgtaccac attgatattt taaagacgct tggaacgtat actgccatcg cgctggagaa
     2401 tgccaatctg tatgagaatc tcgaagtaaa agttgccgaa cgcacgcttg aggtggttgc
     2461 gcagaaagaa gagatcgaaa agaaccaccg cgataccgaa ttgctcagtg ccatcggaca
     2521 ggaaattact tcttccctga gtgttgaagc gatcaatcag caggtgtttg agaaagtaaa
     2581 tacgatgatg gacgcacctt cattcggtat cggtttgtat gatgctgcga aggatgcgat
     2641 tgtttttccg ggatacattg aagagggact ggaagaggaa gttatctata cctccgatga
     2701 tcaggatcgg ctggcatggc tttgtttcaa tcaggaacgc gaaataatga ttggcgatta
     2761 cgatgcagaa cttaccaatt atgttgcacg aagaaaagca gccgtagcag ggcgccttcc
     2821 gaattcggtg atttatattc cgctaaaaac caccaataaa aaaatcggtg tcctcaccgc
     2881 acagagcttc agtaaaaatg cctatactga ataccatctc aacttattga gaaatctttc
     2941 agtgtacgtg gcaattgctt tggataatgc cggattgtat gagggactgg aagacaaagt
     3001 ggagcaacgc acagcggaga tcagaaaagc atacgataat actcggttgc ttgcacagat
     3061 tcagcaggat attatttctt cgctttcggt ggaaacaatt atctccaagg tttacgagaa
     3121 tgtaaatacc ttgatggacg ccaccagttt tgggatcgga atttttgatg aggaatcgcg
     3181 tcacatcatc atgcccggtt ttattgagag cggtgtacgc atggaagaat tttcctatag
     3241 tgtagacgat ccaaaccgtc ttgcggcatg gtgcttcaat aatcagaagg aaatcttcat
     3301 caataattac tatgaagaat actccaaata cattaccgga ataaaaaagg caatctcagg
     3361 aaaagactcc acttcgatta tatatcttcc gctgatggcc aatggcaaac agataggggt
     3421 gattaccgtg cagtcttacg aactcaatgc ctataccgag tatcatcttg atctcttacg
     3481 cagtttagcc gcctctgcag cgattgcaat cgacaatgcg cgattgtatg agaacctcga
     3541 atacaaagtg cgggagcgga cagctgaggt ggtaaaacaa aaagaagtaa tcgaggaaaa
     3601 gaacaagcac atcaccgata gtatcgttta cgccaaacga atacaggaag cgattcttcc
     3661 tccggaggat gtcttcaaac tgcatttgcg gaattcgttt gtgttgtaca aaccaaaaga
     3721 catcgtctcc ggcgattttt actggatcga acgcaaggag aacaaaatac tttttgctgt
     3781 agtggattgt accggacacg gcgttcccgg agcctttatg agtatcattg gttacaacgg
     3841 actcaatcag atcgtcaacg aatacaacat cactcagcct gcggagattc tgaataatct
     3901 caaccgcatc atttcggcaa cactcaaaca gcgcgcagaa gaatccaaga tccgcgacgg
     3961 aatggatctt tcggtgtgca gcattgatct cgaaaaccac aaactcgaat acgccggtgc
     4021 caacaatccg attttcattg tgcgcaacaa tgaagtcatt gaagtaaaag ccgacaaaca
     4081 acccatcgga aattttgtgg gcgaagaaga ttttcgtttc accaacaaag agattgatct
     4141 tctccccaat gatcgtctgt acgttttctc cgacggttac gccgatcagt ttggcggacc
     4201 aagaggcaaa aaactcaagt actccacctt ccgcgatctc ctcatcgaga actgcgatct
     4261 ccccatggaa gaacaaaaac gtgtcctcga tcaaatgttc gaaacctggc gcggcgacct
     4321 cgaacagatt gatgatgtct gcgtgatcgg agtgggca
//
LOCUS       DAOMYG010000281         4347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_7107_length_4347_cov_9.329683, whole genome shotgun sequence.
ACCESSION   DAOMYG010000281 DAOMYG010000000
VERSION     DAOMYG010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7107_length_4347_cov_9.329683"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PK610_09435"
     CDS             <1..625
                     /locus_tag="PK610_09435"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRJ36199.1"
                     /translation="QWGNQYDGMAYNSSLPAGEIGAGNFSNSYLDLGTGLVYTYTQGE
                     KNLTGNNQISATAGIAMFHVHKPDYSFLGDGSENLYRKFVIHGNSLFGIAHSRISVIP
                     GFQYKIQGGARELVFGSSVRYRIMEDSHFTGFVTGSSLSLGFYYRNRDAVILTSLLQF
                     GGYGIGISYDLNVSDLTQASNGRGGLEIALRYIYPSGNKNLKSSRFL"
     gene            complement(1020..1721)
                     /locus_tag="PK610_09440"
     CDS             complement(1020..1721)
                     /locus_tag="PK610_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36200.1"
                     /translation="MRFWQRLRYYMIGVGLGLLVTFFFFRNRGCGWLPQNRVLDRLES
                     SVILRTDSMACVLQRCNAISDEDVFHLLREGDVVFSEGRVDVYPKEYVVASNRKADGT
                     PYKLRFALYDTTSVILGVENPASCTCKDFADTALHILSMPEKMLKEMFLNKEFTITTE
                     GKCMMDCIGVSAEDVVDLIQKGGINNETSTPFSFPHPRYVVINKEYTILVEMTKEKTR
                     ILSIQREGKDCACEH"
     gene            complement(1752..2972)
                     /locus_tag="PK610_09445"
     CDS             complement(1752..2972)
                     /locus_tag="PK610_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRJ36201.1"
                     /translation="MKISQEALKSLAMEAVMIPQEEMLEIGRKRKNLYIGIPRETSFQ
                     ENRVALVPDAVALLVSNGHRVLVETNAGKASNFSDNEYSEAGAQIAYDTAEVYKADII
                     MKVAPPSSEEIALMQPRQTLISALQLSVQPKDTLKKLMSKKITAIAWEHIRDEEGIFP
                     VVRSMGEIAGTASILIAAEYLSKTNNGKGMIMGGVAGISPTEVVILGAGTVGEYATRS
                     ALGLGAIVKVFDSSLYRLRRLQNDLGVRLNTSILQPRALEKSLKTADVVIGAIRAKGG
                     RTPCVVSETMVDNMREGTVLIDVSIDQGGCFETSRVTTHTNPVFDRNGVIHYCVPNIP
                     SRVPRTASIALSNIFGPLMIAIGDKGGSDKLIRSDLGFRAGVYILNGTLTNPILGEAF
                     GLPYKDIDLLSAGL"
     gene            complement(2969..3397)
                     /gene="tsaE"
                     /locus_tag="PK610_09450"
     CDS             complement(2969..3397)
                     /gene="tsaE"
                     /locus_tag="PK610_09450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRJ36202.1"
                     /translation="MSSQLQFTVERVEDLDKLALTLLDFAGDEHIIAFEGEMGAGKTT
                     FIKSICKALGVLDITSSPTFSIVNHYLAPRGNVYHFDFYRLNDPSEALAIGVDEYFSD
                     GSFCLIEWPEKVGNLLPSGHVKVRIEPLSSTTRTITFYRP"
     gene            3450..3866
                     /locus_tag="PK610_09455"
     CDS             3450..3866
                     /locus_tag="PK610_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010134053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRJ36203.1"
                     /translation="MSKRLLITHLCISPGSMRIDKFLWSIRVYKTRTQATEACKAGKV
                     LVNGLPAKASALIKIGELVSTRYESIYKKFEVLAFPPSRVAAKNVELYVIEKTDPADI
                     EKLKLARLNKSAFRDPGKGRPTKKDRRDINRFFLED"
     gene            complement(3872..4243)
                     /locus_tag="PK610_09460"
     CDS             complement(3872..4243)
                     /locus_tag="PK610_09460"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRJ36204.1"
                     /translation="MKENIGLRIKIARVARRWTQQDLADKVNKTRPLITRIETTGLGH
                     PHTIKQICKVLGIAVDEEGWATERGKEYTYNTELLKLKEDNELLLVLTRSQLETITDL
                     RNEVLVLKKKVEQLQRKLLRK"
BASE COUNT         1288 a         1019 c          857 g         1183 t
ORIGIN      
        1 gcaatgggga aatcagtacg atggaatggc ctataattct tctcttcctg caggagaaat
       61 cggagcaggt aatttttcca acagctatct cgatctggga accggattgg tatatacgta
      121 tacacaaggc gaaaaaaacc tgaccggaaa taatcagatc agtgctactg caggtattgc
      181 catgtttcac gttcacaaac ctgattattc attcctgggt gatggaagcg aaaatctgta
      241 ccgcaaattt gtgatccatg gtaattcact tttcggaatc gctcattcgc gcatatcggt
      301 cattcccggt ttccaataca agattcaggg tggggcaaga gaattggttt tcggatcaag
      361 tgtacgttac cgcattatgg aagattctca ctttacagga tttgtaaccg gatcgtcgct
      421 ttcactggga ttctattacc gaaaccgtga tgctgtcatt ttaacttcac tgcttcagtt
      481 tggcggatat ggtatcggta tttcctacga ccttaacgta tctgacctaa cacaagccag
      541 taatggccgc ggtggacttg aaattgcatt gcgttacatc tatcccagcg gaaataaaaa
      601 cctgaagtct tccagatttt tgtaaattgt attcatgtaa gatgcgtaat acgacgttat
      661 aactactcgg tggaggtatc atctatacaa agatgcatcc ctccaccgat tctatttctt
      721 aggaaggcat cgcgatctgc acgaaaaaac attgcctctc atccgaggaa aaaagatggt
      781 cgccctgatc gatatccggg ggggtgtctt aatatgagta atgaagccaa acaacttgca
      841 gcaaatcaca aggaactgaa tgaatgcaga atcgccgaag ctattttgac cgattcactt
      901 gccattcgaa ttgcggcaaa tttttacttt aagttattcc gaccaccaat tccaatgaaa
      961 ttattcagca aggaaaaaaa agctattgaa tggttaagcc agtttccggt aagcgaaaat
     1021 caatgttcgc aggcacaatc tttaccctcg cgctgaatac tcaaaatccg ggttttctct
     1081 ttggtcattt ctaccaaaat cgtgtactct ttattgataa ccacataccg tggatgcgga
     1141 aaggaaaaag gagtgcttgt ctcattattt attccgccct tttggataag atcaaccaca
     1201 tcttcagcac ttacaccaat acaatccatc atgcattttc cttctgtggt aatggtaaac
     1261 tccttattta aaaacatctc tttgagcatc ttttcaggca ttgacaaaat atgcaaggcc
     1321 gtatccgcaa aatccttaca ggtacaggag gcaggattct caacgccaag gatcacactc
     1381 gtggtatcgt aaagtgcaaa acgcagctta tagggagtac catcagcttt tcggttggag
     1441 gccactacat attctttggg atacacatcc actcttcctt cactaaaaac aacatcgcct
     1501 tcacgcaata agtgaaaaac atcttcatcg cttatcgcat tgcatcgctg cagtacacaa
     1561 gccatcgaat cagtccttaa aataacagac gattcaagcc gatccaaaac gcgattctgc
     1621 ggcaaccaac cacatcctct gttccgaaag aaaaagaaag ttaccaatag accaagtcca
     1681 accccgatca tgtaataccg taaacgctgc caaaaacgca tggacgaaga taagtgttta
     1741 aatcagtccg atcagagtcc tgccgagagc agatcaatat ccttgtaagg caaaccgaat
     1801 gcctcgccaa gaatcggatt ggtgagggtt ccattcagaa tataaactcc cgcacgaaaa
     1861 ccaagatcgc tgcgtatcag tttatccgaa ccacctttat caccgattgc gatcatcagc
     1921 ggaccaaaaa tattactcaa cgcaatagat gcagttcgag gaacacgtga aggaatattg
     1981 ggaacacaat aatgaatcac accgtttctg tcgaatacag gattagtgtg agttgtaact
     2041 cttgatgttt caaaacaacc accctggtcg atggagacat caataagtac ggttccttcg
     2101 cgcatattat caaccatcgt ttcagaaacc acacacggag ttcgtccgcc tttagcacga
     2161 attgcaccaa taacaacatc cgctgtttta agcgattttt ccaatgcacg aggttgaaga
     2221 atacttgtat ttaaacgaac acccagatcg ttttgcaagc gacgtaaacg gtacaatgag
     2281 ctgtcgaata ctttcacaat tgctcccaga cccaaagcgc ttcgggttgc atattcacca
     2341 acggtaccgg cacccagaat tactacttcg gtaggagaaa ttccggccac accacccatg
     2401 atcataccct tgccgttgtt ggtcttggat aaatattcag cagctataag aatacttgca
     2461 gttccagcaa tctcgcccat tgatcgaaca acaggaaaaa tcccttcttc atcacgaata
     2521 tgctcccatg caattgcagt gatctttttg ctcatcaatt tcttaagcgt gtctttaggc
     2581 tgaacgctca gctgcaatgc agagatcaag gtctgacgcg gctgcatcag cgcaatctcc
     2641 tcagagctgg gaggagctac tttcataatg atatcagcct tgtaaacttc agctgtatcg
     2701 tatgcaattt gcgctcctgc ttcgctgtat tcgttatccg aaaaattaga tgcctttccc
     2761 gcattggttt ctacgagtac tcgatgacca ttactcacca ataaggcaac agcatccggc
     2821 acaagcgcta cacggttctc ctgaaaggat gtttcacgtg gtattccgat ataaagattc
     2881 tttcgcttcc ttccgatctc caacatttct tcctgtggga tcataacagc ctccatggcc
     2941 agtgacttca atgcttcctg cgaaattttc atgggcggta aaaggtaatg gtgcgagtag
     3001 tagaagataa tggttcaatc cgcactttta cgtgaccgga tggcaaaagg tttccgactt
     3061 tttcgggcca ctcaatcagg caaaaactac cgtcggagaa gtattcatcc accccaatag
     3121 ccaatgcctc cgatgggtcg ttgagtctgt aaaaatcaaa gtgatacacg tttcctctgg
     3181 gagcgagata atgatttaca atggaaaaag tgggacttga tgtgatatcc agcacaccaa
     3241 gcgctttgca aatggacttg ataaaagtgg ttttgcctgc ccccatttcc ccttcaaagg
     3301 caatgatgtg ttcatcgcct gcaaaatcca gcagagtaag agcaagttta tcaagatctt
     3361 ccactcgctc cacggtaaat tgtaattggg atgacatagc gcaaaaatac ccagcaatat
     3421 ggatttgaaa aatcaatcct gtcaaaagca tgagcaaaag gctgttaatt acgcatcttt
     3481 gcataagccc cggtagtatg cgcatcgata aatttctctg gagtattcgt gtttacaaaa
     3541 cccgaacaca agcaactgaa gcttgtaagg cagggaaagt gcttgttaac ggattacccg
     3601 caaaagcctc ggcgctgata aaaatcggtg agctcgtttc tacccgttac gaatcgatct
     3661 acaaaaaatt tgaagtcctt gcatttccgc catcgcgcgt tgctgcaaaa aatgttgagc
     3721 tttacgtcat cgaaaaaaca gatcctgccg atatcgaaaa actaaagctg gcacgactga
     3781 ataaatcagc tttccgcgat ccgggaaaag gacgaccaac aaaaaaagac cgccgcgata
     3841 tcaatcggtt tttccttgaa gattaagtcc cctatttacg caaaagttta cgctgtaatt
     3901 gttctacctt tttcttcaat acaagtacct catttcgcaa atcagtaatg gtttcaagct
     3961 gagaacgggt tagcaccaac aacaattcat tatcctcttt caacttcagc aattcggtat
     4021 tgtaagtata ttcttttcct ctctcggtag cccatccctc ttcatccaca gcaattccga
     4081 gcactttgca gatttgtttt atggtatgag gatgtcccaa acctgttgtt tcaatgcgcg
     4141 taatcagagg tctagtttta tttactttat cagcaagatc ttgctgcgtc cagcgacgtg
     4201 caacacgggc aattttaatc cgcagaccaa tattctcctt catcacccaa agatgacgtg
     4261 gatatgattg tcatttggtc aatttttgtt accaaatgta aattctgcaa atacaggaat
     4321 tcaggagcct atttacaaaa tgtaata
//
LOCUS       DAOMYG010000282         4343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_7112_length_4343_cov_12.008162, whole genome shotgun sequence.
ACCESSION   DAOMYG010000282 DAOMYG010000000
VERSION     DAOMYG010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7112_length_4343_cov_12.008162"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..2005)
                     /locus_tag="PK610_09465"
     CDS             complement(89..2005)
                     /locus_tag="PK610_09465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ36205.1"
                     /translation="MKKIYFAAGLVALAQLGMAQSRVGMELKNPQLRAPQTQIADGSV
                     AGQTGNRALTILHSETFNSTAGNLPAGWSVTDSSSPASGVVWKWTNVGHTGSYPTAAI
                     QSATASDGWMIVDSDLDGDASTDEHTVLISPAYNVSTEPNVAITFSQMMREFQNDICN
                     VAVSGDNGATWTYFTINEGIGQSGTANPDMVFINVSSICGNASQMRVKFSWQGAWDYG
                     WQIDDLAIVIPPDNEIVLNGVDFYTETPSIIWPENIRYTMYPTTQRDPAGVTFRAVMS
                     NNGAQSQTGVTSTVQVRDASTTVVYTGVTTGLNLNANARDTAYSTTSFALPATAMNHT
                     VDYAINYPNLAQDEDLTNNTASYGPINVTPFTFGMDRNQYAGTGLYGGTSGGNDYAYR
                     MGNHFMVFNNQQLQGIQVAFSASTIVGTTVYPFVIEIDDQATSFQDLYANFIYDGGGT
                     AYERTLVASNISTANNMVLYNFDLPNTVNLVAGKEYIIGVGTYGGPATTDRVVIMNGE
                     RNHPGAAHTVLYTTGSSGLQWFYTESTPVIRAMFDPTIGVNEVNNAASVNVYPNPANN
                     IATVEVALTESAVVMVEVMDLTGKLVASNSFGNLSAGSNRLNLNVSDLSEGTYFCTIV
                     INGERITKKMVVTH"
     gene            complement(2139..>4343)
                     /gene="gcvP"
                     /locus_tag="PK610_09470"
     CDS             complement(2139..>4343)
                     /gene="gcvP"
                     /locus_tag="PK610_09470"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HRJ36206.1"
                     /translation="VYDYADFVKQAKANKTAVAVAADLMSLALLTPPGEWGADVVVGN
                     TQRFGVPMGYGGPHAAFFACREDFKRLIPGRIIGVSVDTNGNPALRMALQTREQHIRR
                     EKATSNICTAQALLAIMAGMYAVYHGPDGIKGIAQKIHYSASTLASGLSALGYKIANQ
                     RWFDTITVSGADVEKVRSAAIAAGINFAYNGNEISIALDQTVSVNDINDILAVFSTVA
                     GKAPVTVKAIEEKTPVSGSLSRTSSYLQHPVFSSYHSESEMMRYIKKLENRDLSLVHS
                     MISLGSCTMKLNAASELLPLTWPEFANIHPFVPLDQAAGYQEVFRELDKDLSDITGFA
                     AMSFQPNSGAQGEYAGLMVIRAYHISRGDAHRNIALIPSSAHGTNPASAAMAGMDIVV
                     VKCDENGNIDIADLKAKAELHKDKLSCLMVTYPSTHGVFEEGIIEITSIIHANGGQVY
                     MDGANMNAQVGLTSPGNIGADVCHLNLHKTFAIPHGGGGPGMGPIGVAKHLVPFLPGH
                     AVVATGGDQSIPAVSAAPWGSALILLISYGYIKMLGSEGLTDSTRFAILNANYMKERL
                     KEHYPILYVGKNGRVAHEMILDCREFKRTAHVEVADIAKRLMDFGFHAPTVAFPVVDT
                     LMVEPTESESLAELDRFCDAMIAIRNEIREIADGAYDQENNVIKNAPHTARVCVSDEW
                     KFKYSREKAAFPLPWVRETKFWPSVSRIDNAYGDRNLVCSCLPMEAYETAEA"
BASE COUNT         1280 a          988 c          956 g         1119 t
ORIGIN      
        1 catgcatccc ctgatgcagg ctgaaaaatc aaagcaaaaa aaatcgccct tccgtttccg
       61 gaagggcgat cttaaaaatg attttctatt aatgagtaac caccattttc ttagtgatgc
      121 gctcaccgtt gataacgata gtacagaagt aagtaccttc tgaaagatcg ctaacattaa
      181 ggttaagacg gttagaacct gcagacaggt taccgaagct gtttgaagca accagcttac
      241 ctgtaagatc cataacttct accataacaa ctgcgctttc agtaagagca acctctactg
      301 tagcaatgtt gtttgcaggg ttaggataaa cgttcacaga agcagcattg tttacttcgt
      361 taactccgat agtaggatcg aacattgcac gaataactgg agtgctttca gtataaaacc
      421 actgaagtcc tgaagaacct gttgtataaa gaacagtatg cgcagcacca ggatggttac
      481 gctcaccgtt catgataaca acacggtcag ttgtagcagg accaccataa gttccaacac
      541 caatgatgta ttccttacct gcaaccaggt taactgtgtt aggaaggtcg aaattgtaaa
      601 gcaccatgtt attggcagta gaaatatttg aagcaacaag cgtgcgctca taagcagttc
      661 caccaccatc gtaaataaag tttgcataaa gatcctggaa agatgtagcc tgatcgtcaa
      721 tttcaattac gaaaggataa accgttgttc ctacaattgt acttgcagaa aatgcaacct
      781 gaataccttg caattgctga ttattgaaaa ccataaagtg attccccata cggtaagcat
      841 aatcattacc tccagaagta cctccataaa gaccggttcc tgcgtattgg ttacggtcca
      901 taccgaaggt aaacggagtt acattgattg gtccataaga agctgtattg tttgtcagat
      961 cttcgtcctg tgcaagattt ggataattga tcgcgtaatc aacagtgtgg ttcatagctg
     1021 tagcgggaag tgcgaagcta gttgtagagt aagccgtatc gcgtgcgtta gcattcaggt
     1081 taagaccggt tgtaacgcca gtgtacacaa ctgtagttga tgcatcacga acctgaacag
     1141 tagaggtaac accggtttgc gactgagcac cgttgttaga cattacagca cggaaagtaa
     1201 caccagcagg atcacgctgt gtggttgggt acattgtata acggatgttt tcaggccaaa
     1261 tgatggaagg agtttcagtg tagaagtcta ctccgttaag cacgatttca ttatccggag
     1321 gaataacgat agcaagatcg tcgatctgcc agccgtaatc ccatgcaccc tgccaagaga
     1381 acttaacgcg catttgcgaa gcatttccac aaatagaaga tacgttaatg aaaaccatgt
     1441 caggattagc agtaccggac tgaccaattc cttcattgat ggtgaaataa gtccaggtag
     1501 caccgttgtc accggaaaca gcgacattac agatgtcgtt ctggaactca cgcatcattt
     1561 gagagaatgt aattgcaacg tttggctctg tgctcacatt gtaagcagga gagatcaaca
     1621 cagtgtgctc atcggtagag gcatcaccat caaggtcaga atcaacaatc atccatccgt
     1681 cagaagcagt agccgactgg atagcagcag ttgggtaaga tccggtgtgg ccaacgttgg
     1741 tccacttcca aacaacacct gaagcagggc tggaggagtc ggtaacagac caacctgcag
     1801 gtaaattacc cgcggttgag ttaaacgttt cagagtgaag gattgtgagg gcacggtttc
     1861 cggtttgacc ggctactgaa ccatcagcaa tttgggtctg gggagcgcga agctgtgggt
     1921 tcttaagctc cattcccacg cgggactgag ccatacccaa ctgagccaac gcaacaagac
     1981 cagcagcaaa gtagatcttt ttcatagcag tgagaggttt attgatttat ttgtttgact
     2041 gtttattcga acaaaaatat aaattttttc cggccgtgaa agtggttcgc tgtaggaacg
     2101 gctgttcaca gttattggga ggtttaagta attattaatc aagcctctgc cgtttcatat
     2161 gcctccattg gcaaacaact gcaaaccagg tttctgtcgc cataggcatt atcgatacgg
     2221 cttacacttg gccagaattt ggtttcgcgt acccatggta atgggaaagc agctttttcg
     2281 cgtgaatact tgaatttcca ttcgtctgat acacaaaccc gtgctgtatg gggagcattt
     2341 ttaatcacgt tgttttcctg atcataagca ccatcagcaa tttccctgat ttcattgcga
     2401 atggcaatca tagcatcaca aaaacgatcc agctctgcca gtgactcgct ctctgtaggt
     2461 tctaccatga gggtatctac aacaggaaaa gcaacagttg gtgcatgaaa accaaaatcc
     2521 atcaaacgct tggcaatatc tgccacctca acatgcgcag tgcgtttaaa ctcacggcaa
     2581 tcaagaatca tttcatgtgc aacgcgacca ttctttccaa catacagaat agggtaatgc
     2641 tctttcaatc gctccttcat gtagtttgca ttgagaatgg caaaacgcgt gctatcggtc
     2701 aatccttctg agccgagcat ttttatatac ccgtagctga tcagcaaaat caatgcactt
     2761 ccccagggag cagcagaaac tgcaggaatc gactgatcgc ctcctgtggc aacaaccgca
     2821 tgtccgggaa ggaatggaac aaggtgtttt gctacaccga taggacccat accggggcca
     2881 cctccaccgt gaggaatagc aaatgttttg tgcaaattca ggtgacaaac atccgcaccg
     2941 atatttccgg gagaggttag gccaacctgt gcattcatat tcgcaccgtc catgtaaacc
     3001 tgtccaccat tggcatgaat gatagaagta atctcaataa ttccttcctc aaaaacaccg
     3061 tgtgtagaag ggtaagtcac catgaggcag ctcaatttat ctttgtgtaa ttcggctttt
     3121 gccttgagat cggcaatatc gatattaccg ttttcatcgc atttcaccac aacaatatcc
     3181 attcctgcca ttgcagcaga agcaggattt gttccgtgtg cagaagaagg aatcaaagca
     3241 atattacgat gcgcatcgcc acgggaaatg tgataggcac gaattaccat taaacctgca
     3301 tattcaccct gcgcacctga gttcggttga aaactcatcg cagcaaaacc ggtgatatct
     3361 gaaagatctt tatccaattc acgaaaaact tcctggtaac ctgcagcctg atccaatgga
     3421 acaaaagggt gaatgttggc aaattcaggc catgtgagcg gtaataattc agatgctgca
     3481 ttcagcttca ttgtacagct tccaagagag atcatggaat gaaccaatga caaatcgcgg
     3541 ttctcaagtt ttttgatgta acgcatcatc tccgattcag agtgataact gctgaagaca
     3601 ggatgttgca aatacgaact tgtacgtgaa agggaaccgg ataccggcgt tttttcttct
     3661 atcgctttaa cggtaacagg agctttaccc gcaacagtgg agaatacagc aaggatatca
     3721 ttgatatcat tcaccgaaac cgtttggtca agcgcaatgc tgatctcgtt tccattatag
     3781 gcgaaattaa ttccggcagc aatggcagcg ctccgcactt tttccacatc tgcaccacta
     3841 acagtgattg tgtcgaacca acgctggttt gcaattttat aaccaagagc agataaacct
     3901 gatgccaatg tacttgcaga gtaatgaatt ttctgtgcta tccctttgat accatcagga
     3961 ccatgataaa cagcatacat tcctgccata attgccaaca atgcctgtgc agtacaaatg
     4021 ttggaagtag ccttttcgcg acggatatgc tgttcgcgcg tttgcaatgc catacgcaat
     4081 gcaggattac cattggtatc tacagaaacc ccaatgatac gtcctggaat cagtcgctta
     4141 aaatcttcgc ggcaagcaaa gaaagcagcg tgcggaccac catagcccat gggtacacca
     4201 aagcgttggg tattacccac aacaacatcg gcaccccatt cgccgggagg agtgagtaat
     4261 gcaagcgaca tcagatcggc tgcaacagca acagctgttt tgtttgcttt tgcctgtttt
     4321 acaaaatcag cataatcata cac
//
LOCUS       DAOMYG010000283         4311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_7180_length_4311_cov_8.736607, whole genome shotgun sequence.
ACCESSION   DAOMYG010000283 DAOMYG010000000
VERSION     DAOMYG010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7180_length_4311_cov_8.736607"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..542)
                     /locus_tag="PK610_09475"
     CDS             complement(21..542)
                     /locus_tag="PK610_09475"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRJ36207.1"
                     /translation="MASKKKFARETLAVTTKMVLPNDTNTLGNLFGGQLLAWMDEIAA
                     ISAHRHSKRVVVTASISNVSFNSPIRHAEIVTLEAKVSRSFTSSMEVVVDVFVEDHVT
                     GFRKKCNEAIYTFVAVDQNGGPIEVPELVPETEEENKRYSGALRRRQLSLILAGKMSP
                     DEATELRALFVKD"
     gene            605..1144
                     /locus_tag="PK610_09480"
     CDS             605..1144
                     /locus_tag="PK610_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HRJ36208.1"
                     /translation="MSYLIFKSLHLIFMVTWFAGLFYIVRLFIYQIEAMDKTEEEKKI
                     LLPYLKKIQRPLWYGITWPGMILTWIFGVSMLVDNPQFLKMPYIHIKLSLVFGLTVYH
                     VYCHRIFKQLQTEKRPHGSVFLRYWNEVATLFLFTIVFVIVMKNFLNWMYLGAGLLVL
                     SGLIVWGVIAYKKRRARQS"
     gene            complement(1145..2428)
                     /locus_tag="PK610_09485"
     CDS             complement(1145..2428)
                     /locus_tag="PK610_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ36209.1"
                     /translation="MKKIFSLFVFLLFAFGANAKEYKSVLLMNGTAHLGTGEVITHSL
                     VGIRDGKIVLVANALITNPNKSEYDTVINIEGKHVYPGFIATDVTLGLIEIESVRATA
                     DFAEVGAYNPNVRAVVAYNTDSKVIPTVRSNGVLISQIVPRGGVLSGTSSIMRLQGWN
                     WEDAALQNEDGVHMNWPRFFQNTGWWAEPGETKANDRYQERLDEMKKFFREAKAYAEV
                     KEHERIDVRFEAMRGLFDGTKNLYIHASFVKEITDAVFFCRDLKITKIVIVGGYDSWM
                     VAEMLKENKVSVILQRVHELPSRPEDDIDLPYKLPALLHKAGVLFCLGNEGDQEAHRT
                     RNLPFLAGTACAYGLDKEIALMAVTLNAARILGVSDRIGSLAQGKEATLFVSEGDALD
                     MRTNKVTMAWIQGKAVDLNDHQKELYKRFSDKYRK"
     gene            complement(2437..>4311)
                     /locus_tag="PK610_09490"
     CDS             complement(2437..>4311)
                     /locus_tag="PK610_09490"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRJ36210.1"
                     /translation="VDKQLGTLEKGKVANFIICSEQIFSEKASIQQNWVQGNLFVIQV
                     EDPLDIRGDYDVNIENNIYSLSVKGGLQELQASLKVDTTQVKAKVSLKNRNVSVSFIV
                     KDKNYNGAVRLTGVVHQDGGVWDGNGELPNGNRIKWNAIRKAKFKEENKPSVAPKDSL
                     NQGRVWFPVMAYGWDSLPRQGQVLIKNITVWTNEKEGVLKNTDVLLRDGKIFKIGRIL
                     DVVDKSTWVIDGTGKHLTAGIVDEHSHIAISGGVNEGTHAVTAEVNISDVVNSDDVNI
                     YRQLGGGVTAAQLLHGSANPIGGRSALIKLKWGHTPDEMLIKDAPGFIKFALGENVKQ
                     SNWGEQFSTRYPQTRMGVEQVFYDAFLQAKAYEKEWKKVEQVKGKFGGELPRRDIQMD
                     VLVEILNSKRFISCHSYVQSEVNVLMKVADSMGFKINTFTHILEGYKVADKMLAHGVA
                     ASTFSDWWAYKYEVIEAIPYNAALMHKVGVLTGINSDDAEMARRLNQEAAKAVKYGDV
                     SEEEALKMVTLNPAKMLHLDHRIGSIKVGKDADLVIWSDHPLSIYAKAERTFIEGVCY
                     FDIDRDRLLRERNMQERNRLIGKMIAAKQGGAPTQRPQRPSRRLYHCESLEEYNEEFE
                     "
BASE COUNT         1121 a         1038 c          836 g         1316 t
ORIGIN      
        1 gccaatcgtt tagcggaaca ttaatctttc acaaacaatg ctcttagttc agtagcttca
       61 tcagggctca ttttcccggc aagaataagg gataactgac gacgacgcag agcaccggaa
      121 taacgtttgt tctcctcttc cgtttcggga acaagctcag gaacttcaat tggaccacca
      181 ttttgatcaa ctgccacaaa ggtatagatc gcctcgttac actttttgcg gaatccggtt
      241 acatgatcct caacaaaaac atcgaccacc acttccatag agctggtaaa cgatcttgaa
      301 acctttgctt caagagttac gatttctgcg tggcgtatag gactgttaaa actaacattg
      361 ctaatagatg ctgtaacaac aacccttttg gaatgcctat gagcagaaat ggcagcgatc
      421 tcatccatcc atgcgagaag ttgtccgcca aataaatttc ccaacgtatt ggtatcgttg
      481 ggcagaacca tttttgtagt tactgcaagt gtttcccgtg caaatttttt cttagaagcc
      541 atgctgcaaa gatacagcga atcggggagt tacggacgct attttcctac ctttgcccca
      601 agtcatgagc tacctcattt ttaaatcgct gcacctgatc tttatggtga cctggtttgc
      661 cgggttgttt tatatcgtca ggctgttcat ttaccaaata gaagcgatgg acaaaacgga
      721 agaggaaaaa aagattctgc ttccctattt aaaaaagatt cagcgtcccc tttggtatgg
      781 cataacttgg ccgggaatga tcttgacctg gatttttggt gtttctatgt tggttgacaa
      841 ccctcagttt cttaaaatgc cttatatcca catcaagtta agtcttgttt ttggcttgac
      901 ggtctatcac gtttactgcc atcgcatttt taagcaattg caaactgaaa aacgtccgca
      961 cggaagtgtg tttttacgct actggaacga agtggcgacg ctatttctct tcacgattgt
     1021 attcgtaatc gtaatgaaaa atttcctgaa ctggatgtat ctcggagcag gtttgctggt
     1081 gctttctgga ctgattgttt ggggtgttat tgcctacaaa aagcgaagag cgcgtcaatc
     1141 ctgattactt tctgtactta tccgagaatc ttttataaag ctccttctga tgatcattca
     1201 aatcaactgc cttgccctgt atccaagcca tggtgacttt gttggttcgc atatcgagag
     1261 catcaccctc cgacacgaat aaggttgcct ctttgccttg cgctaatgaa ccaatccgat
     1321 cggaaacacc aagtatacga gctgcattaa gtgtaacggc cattagtgca atttctttat
     1381 caaggccata ggcacaagcg gttccggcaa gaaatggaag attccgggtg cggtgcgctt
     1441 cctgatcacc ttcattgcca aggcaaaaca aaacacctgc tttatgcaat aatgccggca
     1501 acttatacgg aagatcaata tcgtcctcgg gtcgagaagg cagttcatgc acacgttgta
     1561 gaattacact gactttattc tccttgagca tttctgcaac catccaggaa tcataaccac
     1621 caacgattac aattttggtg atttttaaat cgcggcagaa gaaaacagcg tcggtaattt
     1681 cttttacaaa cgaagcatgg atataaagat tttttgttcc gtcgaacaga cctcgcatgg
     1741 cttcgaatcg gacatcaata cgttcgtgtt ccttcacttc tgcatatgct ttagcttccc
     1801 gaaaaaactt tttcatttca tcgagacgct cctggtacct gtcatttgcc ttggtctcgc
     1861 ccggctctgc ccaccatcct gtattctgaa aaaagcgtgg ccaattcata tgcactccgt
     1921 cttcgttttg taaggctgcg tcttcccagt tccaaccttg taagcgcata attgaagatg
     1981 ttcccgacaa aacccctccg cgaggcacaa tctgactgat aagtacacca ttggagcgga
     2041 cagtgggaat tacttttgaa tcggtattgt aggcaacaac ggctctcacg ttagggttgt
     2101 atgcacccac ctcagcaaaa tctgcagtag cccgtacgga ttcgatttcg atcaatccaa
     2161 gcgtaacatc agttgcaatg aagccgggat aaacatgttt gccttcgatg ttgatgactg
     2221 tatcgtattc gcttttattg ggattcgtaa ttaatgcgtt cgcaacaaga acaatttttc
     2281 cgtcgcggat acccacaagt gaatgcgtaa ttacttcacc tgtacccagg tgcgctgttc
     2341 cgttcatcaa taaaacggat ttatattctt ttgcgtttgc accaaatgca aacaacaaga
     2401 aaacaaaaag ggagaatatc tttttcataa tcgtaattat tcaaattctt cgttgtactc
     2461 ttcgagcgat tcgcaatggt acaatcgtcg cgatggtcgt tgtggtcgtt gtgttggtgc
     2521 tccaccttgt ttggcagcaa tcattttacc gattagccgg ttgcgttcct gcatgtttcg
     2581 ttcacgaagc aatctgtcgc gatcgatatc gaaataacat acaccttcaa taaaagtgcg
     2641 ttctgccttc gcgtaaatgg aaaggggatg atcactccag atcacaagat ctgcatcctt
     2701 gcccaccttg atacttccga tgcggtgatc caaatgcaac attttcgcag gattcagggt
     2761 gaccattttc aatgcctctt cctcgctaac gtcgccatat tttaccgcct ttgcagcttc
     2821 ctgattcagt cgacgcgcca tttctgcatc gtcggaatta attcctgtta acacacccac
     2881 cttgtgcatt agtgctgcat tgtagggtat ggcttcaatt acctcgtatt tgtatgccca
     2941 ccagtcggag aaggtagacg cagcaactcc atgtgccagc attttgtcgg cgaccttata
     3001 gccttcaaga atatgtgtaa acgtattaat tttaaaaccc atggaatcgg caactttcat
     3061 gagcacgttg acttcacttt gaacataact atggcaggaa ataaagcgct tactattgag
     3121 gatttctacc aaaacatcca tttgaatatc gcgtcgtgga agttcgcctc caaattttcc
     3181 ttttacctgt tcaactttct tccattcctt ttcataagcc tttgcttgca gaaacgcatc
     3241 ataaaacact tgctccactc ccattcgggt ttgaggataa cgtgtgctga attgttctcc
     3301 ccaattcgat tgcttcacat tttctcccaa tgcaaacttg ataaatccgg gagcatcttt
     3361 gatcaacatt tcatcaggcg tgtgtcccca tttcagttta ataagtgccg agcgtcctcc
     3421 aattggattt gcgcttccat gtaataattg tgctgcagta acacctccac ctaactgtcg
     3481 gtaaatatta acgtcgtcgg aattcaccac atccgaaata tttacttcag cagttacagc
     3541 atgtgtcccc tcgtttaccc cgccgctgat cgcaatgtgc gaatgttcat caacgattcc
     3601 tgctgtgaga tgttttccgg tgccatcaat tacccaagtg cttttgtcga ccacatcaag
     3661 gatccttccg atcttaaaaa ttttcccatc acgaagcaat acatccgtgt tctttaaaac
     3721 accttctttt tcgttggtcc aaaccgtaat gtttttgatg agcacctggc cctgtcgtgg
     3781 caatgaatcc caaccatatg ccattaccgg aaaccatact ctgccttggt tcaacgaatc
     3841 ctttggagca acagagggtt tgttttcttc tttgaatttg gctttccgaa tagcattcca
     3901 tttgatcctg tttccattag gaagctctcc gtttccgtcc catacaccac catcctgatg
     3961 aactacacca gttaagcgaa cggcaccgtt ataattttta tcctttacaa taaaactcac
     4021 agagacattc cggtttttta gtgagacttt cgccttaacc tgcgttgtgt ccacttttaa
     4081 acttgcctgt aattcctgca aaccaccttt taccgataat gaatagatat tgttttcaat
     4141 atttacatca taatctcccc ggatatccaa agggtcttct acctgaataa cgaataagtt
     4201 tccctgtacc cagttttgct gaatgcttgc cttttcggaa aagatctgct ctgaacaaat
     4261 aatgaaattt gcgactttcc ccttttccag tgtgccaagt tgtttgtcaa c
//
LOCUS       DAOMYG010000284         4300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_7203_length_4300_cov_12.886219, whole genome shotgun sequence.
ACCESSION   DAOMYG010000284 DAOMYG010000000
VERSION     DAOMYG010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7203_length_4300_cov_12.886219"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PK610_09495"
     CDS             <1..705
                     /locus_tag="PK610_09495"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRJ36211.1"
                     /translation="DEWFKKLFPDSFVLYKPKDIVSGDFYWIEDCTDKLLIAAVDCTG
                     HGVPGAFMSIVAHNGLTMAVNVNKLTRPAEILDYLTLHVNTILSQNLESATVRDGMDI
                     SLCAIDKKTGMVEYAGANNPLIIARKGQQELFEINADKQPVGNFSGVSTRPFTHHEVR
                     VEKGDVIYLFSDGYADQFGGEKGKKFKYSRFKSLLKEFSGKSMQEQLHLLNAQFESWR
                     GDLEQLDDVCVVGIQF"
     gene            802..4239
                     /locus_tag="PK610_09500"
     CDS             802..4239
                     /locus_tag="PK610_09500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ36212.1"
                     /translation="MRHFLLSFVLFFSLTAHAQIDIDHTGSDTYSVFVDFNQTVAQSF
                     TPTVTGRLNQIDLDLEADFCVDNITLAIRFELRQGAGTSGQILDSVTHFLSLPMSRSM
                     YGVFFSSSPQVCAGNIYTIVAVIPNQPFCDIGASAYPTARWFSTNSDTYAGGNIFLNG
                     TPVITEDLYFKTYYNDPFFVTFTPTNATCPGAFNGSITSTPSGGIAPLTYSWMPLGGS
                     SQNPSGLDAGTYSVTVTDAVGCTISDPAVTITTSGPGPVISFTTVTNPTCFGGSDGEI
                     TSSVTGGTGPYTYLWNGTTPTQNISGITQGYYELQATDINGCTGLLGQTLNDPAEIII
                     NFTGTSPNCPTTADGSITTTITGGTGPFNYLWTPGNFTTTGISGLIAGTYSLTVVDAN
                     GCVKMDAYPLTAPSYYIQTGTMVVQPETCQQGNGSITGIYASTLNPPVTYVWSAPNTN
                     NNLFNVPGGNYLLTATDNAGCVDTVTINVPSLGFTLSMNQSSTPASCTGICDGSATVS
                     ANGGTPPYSFVWSNGQTGSVATGLCANTHTVTVSDVNGCSRSTNIGIGQPGPFNVSPT
                     YTEATCGASDGAISLTVVAGGTPPHTYSWTTSVSGSSITGVPAGSYGVTITDANNCQN
                     YRVFSLNSASGPSISNVLTSPTCFGSSNGSIDQTITGGTAPITILWSTGHTGPDLNAI
                     PEGVYDVEISDASGCVIYKTYELFAPAAIEIFPTLTNPSCGVPNGGITLNASGGTSPY
                     TYVWSANAGGAVTPAVSPLSAGIYSVTITDVNGCAFSQSIGLSNDNGPNVSTDALIQP
                     SCQNDDGAIFITASGVPGPFNYSWSGLPYPQNIQADQTDLAPGIYNLSVYASGGCESH
                     MTYSLTVNNSNAQEICLITVDSITNKNVVVWEKTDGLGIAEFELFRETSVPGVFLKIA
                     TIPYNDLSSFNDDVASSSAHAWKYKLVTIDSCGISSRTVASHKTIHMNAELISGGTEV
                     ELIWDNYIGFSYSDVNVLRNHNGTGWVVIATLPATQFTYTDNNLPTNGTLEYSVEAIP
                     TSPCVASRAINHNTTRSNKGSVAAPVGISENENLQHLKIFPNPTSGIFRIEGVYSTEE
                     KINIEVFDMTGRSVYSNLFVVSNQSIQLDLSNYENGLYSIRVSTVSASTTKSLVIQK"
BASE COUNT         1186 a         1028 c          874 g         1212 t
ORIGIN      
        1 gatgaatggt ttaaaaaatt gtttcctgac tcctttgtgc tctacaagcc gaaagacatt
       61 gtaagcggag atttctactg gattgaagac tgtacggata aattacttat tgctgcagtt
      121 gactgcacag gtcatggtgt accgggtgca tttatgagta ttgttgcaca caatggatta
      181 acaatggcgg ttaatgtcaa caaacttact cgtccggccg aaattcttga ttatctcacg
      241 ctacatgtca atacgattct tagtcagaat ctcgaatcgg ccacggttcg cgacggcatg
      301 gacatttcat tgtgtgcgat cgataaaaaa acaggaatgg ttgaatatgc cggcgccaac
      361 aatccgctta tcattgcacg aaaaggccag caggaattgt tcgagatcaa tgccgataag
      421 caacctgttg gaaatttctc cggtgtttct acccgtccat ttacacacca tgaagtaaga
      481 gtagaaaagg gagatgttat ttatctcttt tcagacggat atgctgatca gtttggtggt
      541 gagaaaggaa aaaaatttaa gtattcccga ttcaaatcct tactcaagga attcagcggg
      601 aaatccatgc aggagcagtt gcatttgctt aacgcccaat ttgaaagttg gagaggcgat
      661 ttggagcagc tcgatgatgt atgcgtggtg ggaattcaat tctaacaata gccgtccgaa
      721 tactcctgat ttttatcctt ctttcgttgg atagttcgct caatggctta catttgatta
      781 gcactaacct atatctcccc tatgaggcat tttctacttt cgtttgttct ctttttctca
      841 ctcacagccc atgcccagat cgatattgat cataccggat ctgacacgta cagtgtattt
      901 gtagatttta accaaacggt agcccaatcc tttactccaa ccgttacagg ccggttaaac
      961 caaattgatc ttgatcttga agcagatttt tgtgtggaca atattaccct tgctattcga
     1021 tttgaattgc gtcagggagc aggcacaagc ggtcagattt tggactccgt tacccatttt
     1081 ctgagccttc ctatgtcaag atccatgtat ggcgttttct tctcgagttc cccacaggtt
     1141 tgcgccggaa acatttacac catagttgca gtaataccta accaaccctt ttgtgatatt
     1201 ggtgcttctg cttatcctac tgcgagatgg ttttcaacca attctgacac ctacgccgga
     1261 ggtaacattt ttttgaatgg aacaccggtc attactgaag atctttactt caaaacctat
     1321 tacaacgatc cgttttttgt aaccttcacg cctaccaatg ctacatgtcc gggggcattc
     1381 aacggatcaa ttacatcaac tccctcaggc ggaattgcac cgctcaccta ttcgtggatg
     1441 ccattgggag gaagcagtca gaacccaagc ggactagatg caggaacgta cagcgtaact
     1501 gttactgatg cggtaggttg taccatttct gatcctgctg ttacaattac aacatcaggg
     1561 ccggggcctg ttatcagttt tacaaccgtt accaacccca cttgctttgg cggatctgac
     1621 ggagaaatca catcttctgt aacaggggga accggtccgt atacttattt atggaacgga
     1681 actactccaa cacaaaacat ttccggaata actcaaggtt attacgaact tcaggcgacc
     1741 gacatcaatg gatgtactgg cttactaggt cagacgctga atgatcctgc agaaatcatc
     1801 atcaacttca caggcacatc tcccaactgt ccaacaaccg cagacggatc aataaccact
     1861 acaattacag gaggaacagg tcccttcaac tacctgtgga ccccggggaa ttttacaaca
     1921 accggaatta gcggacttat tgcaggcaca tatagtctca ctgttgttga tgcaaatggc
     1981 tgtgtgaaaa tggatgctta tcctctaact gctccttctt actatattca aaccggaaca
     2041 atggttgttc agcctgaaac ctgccaacag ggaaatggat caatcaccgg aatatatgca
     2101 tccactctca atccacctgt tacctatgtg tggtcggcac ccaatacaaa caacaacctg
     2161 ttcaatgttc cgggcggaaa ttatctgctt acggcaaccg ataatgcagg atgtgtagac
     2221 acagtaacca tcaatgttcc ctctcttgga ttcacccttt caatgaacca atcttcaacc
     2281 cctgcgtcct gtacaggtat ttgcgatggt tctgcaactg tttctgccaa tgggggcact
     2341 cctccctatt cttttgtttg gtcgaatggg caaacaggat cagtcgcaac aggattatgt
     2401 gccaatacgc ataccgttac cgtatcggat gtaaacggat gctcccgctc cacgaacatt
     2461 ggtattggtc aacctgggcc tttcaatgtt tctccaactt acacagaagc cacgtgcggt
     2521 gcttcagacg gtgcaatttc acttacggtt gtggcaggag gaacccctcc tcatacctac
     2581 tcatggacaa ccagtgtttc cggatcatct ataacaggag ttcctgcagg aagctatggt
     2641 gttacaatta ccgatgccaa caattgtcag aactaccgcg tattctcact caacagcgca
     2701 agtgggccaa gtatttcaaa cgtacttacc agcccgactt gtttcggatc atccaatgga
     2761 tccatagatc aaaccataac aggcggaact gcacccatta ccattttgtg gtcgaccgga
     2821 cacactggtc ccgatctgaa cgcgattcct gaaggcgttt atgacgtgga aatttccgat
     2881 gcgtccggtt gcgttattta taaaacttac gaactttttg cacccgcggc aattgaaatt
     2941 ttcccaaccc tgactaaccc ttcctgtggc gtccccaatg gtggaataac gctaaatgct
     3001 tccggcggaa catctcctta cacctatgtt tggagtgcaa atgctggtgg agctgttacg
     3061 cctgcagtat ctccactttc ggcaggaatt tattcagtaa caattacgga tgtaaatggt
     3121 tgtgcattct cgcaatcgat cggactcagt aacgacaatg gtccaaatgt ttcaacggat
     3181 gcgctgattc aacccagctg tcagaatgat gacggagcca tctttatcac agcttcagga
     3241 gtacccggtc ccttcaatta ttcctggagc ggcctccctt atccacaaaa catccaggcc
     3301 gaccaaactg atcttgcacc gggaatttat aatctatctg tttatgcttc cggcggatgt
     3361 gagtcgcata tgacctattc actcaccgta aacaactcaa acgctcagga aatttgcctg
     3421 atcactgtgg attccatcac gaataaaaat gttgttgttt gggagaaaac ggatggtctt
     3481 ggtattgcag aattcgaact tttcagagag acaagtgtcc cgggtgtttt tctcaaaatt
     3541 gccactattc cgtacaacga tttaagttca ttcaatgatg atgtggcctc ctcttctgca
     3601 catgcctgga aatacaaact ggttaccatc gactcctgcg gaatctcttc aagaaccgta
     3661 gcctcccaca aaaccattca catgaatgca gaacttattt cgggaggaac cgaagttgaa
     3721 ttgatatggg ataattacat tggattttct tattcggatg taaacgtatt gcgcaatcat
     3781 aacggtaccg gatgggtggt gattgcaacc ttacccgcca cacaattcac ctataccgac
     3841 aacaaccttc ctactaacgg cacacttgaa tactcggtag aagcaattcc aacatcaccc
     3901 tgtgttgcaa gccgtgcaat taaccacaat actacccgct ccaacaaagg ttctgtagct
     3961 gcaccggtag ggatttctga aaacgaaaac ctacaacacc taaagatttt cccaaatcct
     4021 acatcaggaa ttttccgaat tgaaggcgtt tactcaactg aagaaaaaat caacatagag
     4081 gttttcgata tgacaggaag aagtgtttat tctaatttgt ttgttgtttc caaccaatcc
     4141 attcaactgg atctttccaa ttacgaaaat ggtctttatt ctattcgggt ttcaactgtt
     4201 tcagcaagta caacgaaatc tttagttatt cagaaataac tctttaaaat ttaattaaaa
     4261 cggggcagct gttcagcccc gttttaatta aattttaaag
//
LOCUS       DAOMYG010000285         4249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_7337_length_4249_cov_7.860515, whole genome shotgun sequence.
ACCESSION   DAOMYG010000285 DAOMYG010000000
VERSION     DAOMYG010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7337_length_4249_cov_7.860515"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /gene="mazG"
                     /locus_tag="PK610_09505"
     CDS             <1..703
                     /gene="mazG"
                     /locus_tag="PK610_09505"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRJ36213.1"
                     /translation="QCPWDRKQTLESLRILTIEETYELADAIIENDLPEIKKELGDLM
                     LHLVFYARIASESGAFDIADVLHGVCDKLIERHPHIYGDVKVENEEQVKANWEKIKLK
                     TGNRSVLEGVPVSLPSMVKAVRIQEKAKGVGFDWDHSDQVWEKVKEEIAELKIEVDRK
                     SDKIEEEFGDVLFSLINYARFIGVNPEDALERTNKKFIKRFQFLETESAKDGKKMGEM
                     SLEEMDHYWNKAKSL"
     gene            729..1721
                     /locus_tag="PK610_09510"
     CDS             729..1721
                     /locus_tag="PK610_09510"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HRJ36214.1"
                     /translation="MKKEIMRIPLLTVFVLMASFAFAQEPTKPTEGQQRGVRQRPTSK
                     PQEADCSTDLEYQEKADLVVYRKTGDPFSGLCRTYYEDNRLEREITFVDGKEDGISTT
                     YYKRVEKDPKDAKDPKPGQIMVITEHKLGVPEGTWKYYFENGKLAWSNTYLNGKKEGQ
                     WEFFYENGTPKKEENWKDDKKNGVFIDYFPSGKKKVEINYKDNKMHGAYKQYFENESV
                     FIERNYIEGKEDGEEISYHKNGQVASIRRYKLGQPEGTWRTYFDDGKEKSVETYIKGK
                     KSGEHKEFHREGQLKKRSVYVEGKLTLMEEFDEFGNKIDREAEKKRQEEEMREE"
     gene            1718..2665
                     /locus_tag="PK610_09515"
     CDS             1718..2665
                     /locus_tag="PK610_09515"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRJ36215.1"
                     /translation="MIRLQGDRSTYLFDPANTRTCLRPNGRFSIIYLGKDEKDGATVI
                     VKQLHPRLRRQLRERRRFMKEFHPFWDMQGITRTLDMIITDEDLYLIREYREGLDLQS
                     WIEQGHFDKLRSAAKRDFVLNLLELVAGLHRNGQLHGDIKPSNFIIGPSGEVSLIDLG
                     LAMPLDKRERPSRDASDPLPFSMLYSPPELMLNFPDLLDERSDVFSMGIVLYQLMSGE
                     MPWDEKNPAVLMNLQLVYPIARRSVINRGLFSIIEKMTARYAFPVPPLTLPEDEQREG
                     IQTGMEKRFANLEEVKKELETLLNFQWKKRKWLAFFLGK"
     gene            complement(2671..3522)
                     /locus_tag="PK610_09520"
     CDS             complement(2671..3522)
                     /locus_tag="PK610_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ36216.1"
                     /translation="MKLYVLETGNFKLDGGAMFGVVPKTIWQRTNPADENNMCTWAMR
                     CLLIEDGKRLMLVDTGIGNKQDEKFFSHYFLHGDATLDSSLQKLGFSRDQITDVFLTH
                     LHFDHCGGAIEFDKSKDAFRPAFANATYWSNDRHWGWATQPNPREKASFLKENILPIK
                     ESGQLKMIERKEDLTAEVFPGIDVLFVDGHTDSMMIPHIRYKGKTIVYMADLLPSTGH
                     IPLPYVMGYDTRPLLTISEKERFLKRAADEEFVLLLEHDSVNECCTLQHTEKGVRLKE
                     TFRFSEI"
     gene            complement(3519..4166)
                     /locus_tag="PK610_09525"
     CDS             complement(3519..4166)
                     /locus_tag="PK610_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36217.1"
                     /translation="MELLKYIFFLGIVFIVFSGIWSFFMLIYRLLTGLSERPWFETFL
                     FKILNTYFLVSLCAMVTAEYTAKPGAPRILITLVGISILYAHLAGRLRQTRIMVQMNN
                     FRMENTNINMAWESLLILVAMVYFSFGITHPEILINNANTWFAVSVKDLYDTIIIGWI
                     IAFLGIIMLLVNIMNSIMVTSKAVNWIMEKITGTNRNDRGNKNDQGEYTDYEELP"
BASE COUNT         1295 a          816 c          999 g         1139 t
ORIGIN      
        1 gcagtgtccc tgggatcgga aacaaacact tgaatcgctg cgaattctca ctattgaaga
       61 aacatacgag ttggcagatg caataattga aaatgacctt cctgaaatca aaaaggagtt
      121 gggtgattta atgctccatc ttgtttttta tgcacgtatc gcgtctgaat ccggcgcttt
      181 cgatattgct gatgtattgc acggtgtttg cgataagctg attgaacgcc acccgcatat
      241 ttatggcgat gtaaaagtag agaatgaaga gcaggtcaag gctaactggg aaaagatcaa
      301 attaaaaaca ggtaatcgtt cggtccttga gggcgttcct gtttctttgc cttccatggt
      361 gaaggctgtt cgtattcagg aaaaagcgaa aggtgttgga ttcgactggg atcattccga
      421 tcaggtctgg gagaaagtga aggaggaaat agcagagttg aaaattgaag tcgacaggaa
      481 aagcgataaa atcgaggagg aatttggtga tgtgttgttt tccctgatta attatgcgag
      541 gtttattgga gtgaatcctg aagatgcgct tgaacggacg aacaaaaaat tcatcaaacg
      601 cttccaattc cttgaaacgg aatcggccaa agacgggaaa aaaatggggg agatgagtct
      661 tgaggaaatg gaccattact ggaacaaagc caaatcatta taatcaggca tatttattgc
      721 aattgatcat gaaaaaagaa attatgcgta ttccattgct cactgttttt gttttgatgg
      781 cttcttttgc ctttgcgcag gaaccgacca agccaactga aggtcaacaa cgtggtgtgc
      841 ggcagcggcc tacttctaaa cctcaggaag cggattgcag cacggatctc gagtatcagg
      901 aaaaagcaga tttggttgtt taccgaaaaa caggcgatcc attttcagga ctttgtcgta
      961 cgtattacga agacaaccga ctggaacggg aaataacttt tgtagatggc aaggaagatg
     1021 gaatttcaac cacctattac aagcgtgttg aaaaagatcc aaaagatgct aaagacccca
     1081 agcccggaca aatcatggtg atcaccgaac ataaactggg tgtgcctgaa ggaacgtgga
     1141 agtattattt tgagaatgga aagcttgcct ggtcaaatac ctacctcaat ggaaaaaaag
     1201 aaggtcagtg ggaatttttc tatgagaatg gcacaccaaa aaaagaggaa aactggaaag
     1261 acgataagaa aaacggtgtc tttatcgatt atttccccag cgggaaaaaa aaggtagaga
     1321 tcaattacaa ggacaataaa atgcatggcg catacaaaca gtatttcgag aacgaatcgg
     1381 tttttattga gcggaattat atagaaggaa aagaagacgg agaagaaatt tcgtaccata
     1441 aaaacggaca ggtggcctcc attcgtcgtt acaaactggg tcaacccgaa ggaacctggc
     1501 gcacttactt tgatgatgga aaagaaaagt cggtcgagac ctacataaaa ggaaaaaaat
     1561 ctggagagca taaagagttt caccgcgaag gtcagctcaa gaaaagatcg gtctatgtgg
     1621 aaggtaaact taccctgatg gaggagtttg atgaattcgg gaataagatc gaccgtgaag
     1681 cagagaagaa gcgtcaggaa gaggaaatgc gcgaagaatg atcagacttc agggcgacag
     1741 gtctacctat ctttttgatc cggcgaatac ccgcacttgt ttgcgtccaa acggacgatt
     1801 ctctataatt tatctgggga aggatgaaaa ggatggcgca actgttatcg tgaagcaact
     1861 tcacccgcgt ttacgtcgcc aacttcgtga gcgtagacgg ttcatgaaag aatttcatcc
     1921 cttttgggat atgcagggaa ttacccgcac tctggatatg atcattacgg atgaagattt
     1981 atatcttatt cgggaataca gggaaggact ggatctgcag agctggatcg aacaaggtca
     2041 ttttgataag ttgcgttctg cagccaagcg cgatttcgtt ttgaatctgc tggaactggt
     2101 agccggcttg catcgtaacg gtcagcttca tggagatatc aagccctcta attttattat
     2161 cggtccatcc ggagaagttt ccttaattga tttgggcctt gcaatgccgc tcgataaaag
     2221 ggaacgccct tcacgagatg cttcggatcc tttgccattt tccatgctgt attcgccgcc
     2281 ggaattgatg ctgaatttcc ctgacttgct ggatgaacgc agtgatgttt tctcaatggg
     2341 aattgtgctt tatcaactga tgagtggtga aatgccatgg gacgaaaaaa atcctgctgt
     2401 tctgatgaat ctgcagctgg tttatccaat tgcacgaagg agtgtaatca atcggggatt
     2461 gttttccatt atcgaaaaaa tgacagcgcg ttatgctttc cctgtgccac cacttacgct
     2521 tcctgaagat gaacaacgtg aaggaataca aacaggaatg gaaaagcgtt ttgcaaatct
     2581 ggaagaggta aaaaaggaat tggaaacact gcttaatttt cagtggaaga aacggaaatg
     2641 gctggccttc tttttaggta aatagccctt tcagatttct gagaatcgga atgtttcctt
     2701 tagacgcacg cctttttcag tatgttgcaa tgtgcagcat tcattaacag aatcgtgttc
     2761 caaaagcaaa acaaattctt cgtcggcagc tcgtttcaga aatctttctt tttcgctgat
     2821 ggtgagtaaa ggacgcgtat cataacccat tacataggga agaggaatgt gcccggtcga
     2881 aggtaaaaga tcggccatat aaacgatggt ttttcccttg tagcggatat gcggaatcat
     2941 catactgtcg gtatgtccat ctacaaacaa aacatctatt ccgggaaata cttcggcagt
     3001 taaatcttct tttcgttcaa tcatttttaa ctgaccgctt tcttttatgg gtagaatatt
     3061 ttccttcaga aaggaagcct tttcccgtgg attaggttga gtagcccaac cccagtgacg
     3121 atcattgctc cagtacgttg cgttggcaaa tgcgggtcgg aatgcgtctt ttgatttatc
     3181 aaattcaatg gctccaccgc aatggtcaaa atgcaggtga gtgagaaaca catccgtgat
     3241 ctggtcgcgc gagaagccta atttttgcaa tgaagaatcg agtgtagcat cgccatgaag
     3301 gaaatagtgt gagaaaaatt tctcatcctg cttgtttccg atcccagtgt ctacaagcat
     3361 caaacgcttt ccgtcttcga tcagtaaaca gcgcatagcc catgtgcaca tgttgttttc
     3421 atctgcagga ttggttcgtt gccagatggt tttcggtaca acaccaaaca tggctccacc
     3481 gtcgagtttg aaatttcctg tttcgagtac gtataacttc atggtaattc ttcgtaatct
     3541 gtgtattctc cctggtcgtt tttgttgccg cgatcatttc ggttggtgcc ggtaattttt
     3601 tccattatcc aattcaccgc tttggaggta accataatac tgttcatgat attcacaagt
     3661 agcatgatga tgcccaaaaa ggcaatgatc catccgatga tgattgtatc gtacaaatcc
     3721 tttaccgaaa cagcaaacca ggtgttggca ttatttatta gaatttcggg atgcgtaatt
     3781 ccaaatgaaa aataaaccat cgcaacaagg atcagcaacg attcccatgc catattgatg
     3841 tttgtatttt ccattctgaa attgttcatc tgaaccataa ttcgtgtctg tctcaatcgt
     3901 cctgccaaat gagcgtatag aatactaatt ccaaccaacg taatcagaat gcgtggcgct
     3961 ccgggctttg cggtgtactc ggcggtaacc atggcgcaaa gtgagacaag aaagtaggtg
     4021 ttcagaattt taaacaggaa agtttcgaac cagggacgct cagataaacc ggtaagcagc
     4081 cggtaaatca acatgaaaaa actccagatc ccactaaaga caataaaaac gatcccgaga
     4141 aagaaaatgt actttaataa ttccataggg taaagatatg tttaattggt gaaaagtgac
     4201 aggtgaaaaa tgaaaagtga aaggggtaac taagcaccat tctctgttc
//
LOCUS       DAOMYG010000286         4247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_7340_length_4247_cov_9.673426, whole genome shotgun sequence.
ACCESSION   DAOMYG010000286 DAOMYG010000000
VERSION     DAOMYG010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7340_length_4247_cov_9.673426"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="PK610_09530"
     CDS             complement(<1..1109)
                     /locus_tag="PK610_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRJ36218.1"
                     /translation="MTPKRFHPQDIKAGLVVFLVAIPLCLGIALASGAPAFSGIIAGI
                     VGGIVVGSISGSPLSVSGPAAGLTSIIITALGVLPSFEVFLAAVVIAGLIQLLFGALR
                     LGIFAGFIPSSVIMGMLAAIGLILIMKQIPHAVGFDKDFEGDESFLQADGHNTFSELY
                     YAFANPNFGAVLICLIGIGILLLWDTRFFKTNRILSLIPSALLVVFAGVGLNMFFASS
                     FPDWEMKGDHLVTLPKINSMDSLVAEIRIPDFHTAINLPIVWVTAITLALVASIETLL
                     SIEAADRLDPWNRTTPPNQELVAQGVGNIFSGMLGGLPVTAVVVRSSANITAGARTKM
                     AAITHGFFLLVSIMAIPSLLNLIPKSALAAILLMVG"
     gene            1518..2489
                     /locus_tag="PK610_09535"
     CDS             1518..2489
                     /locus_tag="PK610_09535"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HRJ36219.1"
                     /translation="MIKHILLLAAVLFSGLVLGQEDELKKNAQKLTPGKTDTIQGWKK
                     GGSSSLTFSQVSLTNWAAGGQNSIAFNGALNVYVNYKKGHNTWDNNLDLGFGLVRQGL
                     ADVRKSDDRIDFSSKFGKQAFGKQNLYYATLFNFRSQFAPGYNYPNDSVVISRFLAPG
                     YFTLALGLDYKPVKNMAIFVAPLTGRITVVNDQLLADNGAFGVEKATYDTAGVKITNG
                     KRTFSEFGGYLKFTYKFDIGKSTTYSTRLDIFSNYLRNPQNLDIMWDNNLVIKVSKYI
                     GVTFSALMIYDDDTNIAVDTNSDGVVDGEGPRLQFRQIFGVGFSYKF"
     gene            2489..3178
                     /locus_tag="PK610_09540"
     CDS             2489..3178
                     /locus_tag="PK610_09540"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRJ36220.1"
                     /translation="MKKNWLLPVIALLLITGVGIAIAAYMNSKAYEEADVLPVYNPSQ
                     VNDSLVDPTMHNVGMRHRVGDFSLYDQRGRTITQKDLEGKIYVADFFFTTCKSICPKM
                     TSQLQRVQEKYKSNAQVMILSHTVLPEVDSVETMMNYAMQYGADNDKWLFLTGEKSMI
                     YSMARKNYFIVKEAEQGSGDGDGDFIHTENFVLIDTKKRIRGYYDGTSKEDVDRLLGD
                     MDKLLREEGIK"
     gene            3471..3791
                     /locus_tag="PK610_09545"
     CDS             3471..3791
                     /locus_tag="PK610_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HRJ36221.1"
                     /translation="MKEITEALVKFRNERDWEQFHNPKDLAVALNIESAELLELFLWK
                     KPEEANSEKIKEELADVFAYAFLLAEKYKLDVKDIVLEKIKKNGEKYPVDKAKGSAKK
                     YDEL"
     gene            3788..>4247
                     /locus_tag="PK610_09550"
     CDS             3788..>4247
                     /locus_tag="PK610_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRJ36222.1"
                     /translation="MSMNFDLSIEISEEYDFSSGGLSKIEQNPWVKNQWPLVYFIQNR
                     SEKLAYVGESTNASSRFKNHLSNPQRLKLNKISIIGSDKFNKSATLDLEANLIQYLTA
                     EGSYSLQNGNHGLINHNYYQQDLYKNLFHEVWKKLLEKKIVTKTLSEIENS"
BASE COUNT         1323 a          864 c          969 g         1091 t
ORIGIN      
        1 cctaccatca gcaatatcgc tgcaagtgca cttttgggaa tgagattgag cagcgaagga
       61 atggccatga tgctcaccag caaaaagaaa ccgtgggtga tggctgccat ttttgttcgt
      121 gctcctgcag tgatgttcgc tgaacttctt acaacaacag cagtaaccgg caatccaccc
      181 agcatccccg aaaaaatatt tcctactcct tgcgcaacga gctcctggtt gggtggagtg
      241 gtgcggttcc agggatcaag acggtctgct gcttctatac tcagtaatgt ttcgatagag
      301 gccaccagtg caagcgtaat ggcggttacc cacacaatcg gtaaattaat tgcggtatga
      361 aaatccggta tacgaatttc ggcaacgaga ctgtccatgc tgttgatttt tggcagagtc
      421 accaaatgat cgcctttcat ctcccaatcg ggaaaactgc ttgcgaaaaa catgttgaga
      481 ccaactcccg caaacaccac aagcaatgca gacggaatca gcgagagaat acgattggtt
      541 ttaaaaaacc tggtatccca caacaaaaga attccaatac caatcaaaca aataagaacc
      601 gcaccaaaat tgggattggc aaatgcataa tacaattccg aaaaggtgtt gtgaccatca
      661 gcctgcaaaa aactttcgtc gccttcaaaa tccttatcga aaccaacggc atgcggaatc
      721 tgtttcatga tcaggatcaa tccgatggcg gcaagcatcc ccatgatcac cgaagaggga
      781 atgaatcctg caaaaatacc aaggcggagc gcaccgaaaa gtaactgtat caatcctgca
      841 ataacaaccg ctgcaagaaa cacttcaaag ctgggtaaaa cacccagtgc tgtaatgatg
      901 atggaggtga gtcctgccgc aggaccactt acactcaaag gcgaaccgct tatactcccc
      961 acaacaattc cccctacaat accggcaatg atgcccgaaa atgcaggagc ccccgatgca
     1021 agggcaatac ccaaacaaag aggaatagcc acaagaaata caacaagacc ggctttaatg
     1081 tcttgaggat gaaaacgttt tggtgtcatt gaaggcttgc tgtttgggtt ggaagtaatg
     1141 ctttcaaaga tagtattatt aattaatgaa cggcaatagg gatggatgcg aggatgattt
     1201 tcagtaagcc aatttccaac aagtagggtt taggttggtt ggtggaacca atctgatttg
     1261 gttttttaga gtaaccgcaa atggaaagag ggagaaagca acaggggaag aacactcccg
     1321 cctggagttt tctgcttctg cactgttcac cgaccagtcc cgaccgatcc agccccgacc
     1381 gtacctgccc cgaccgtacc tgccccgacc gtaagcgtcg gggagcgtcg ggatcacctt
     1441 tcactgtttc cacattaacc aaactaattc tttgatgcta aggatataaa taagaagttt
     1501 gcgaaaaaat tattgaaatg atcaaacaca ttttactact tgctgctgta ttgttttccg
     1561 gattggttct tggtcaggag gatgagctga agaaaaacgc acagaaactt actccgggta
     1621 aaacggatac cattcagggt tggaaaaaag gaggctcaag ctcgcttacg ttttcgcagg
     1681 tttcacttac caattgggct gcaggcggac aaaattccat cgctttcaat ggagcactca
     1741 atgtatatgt caattacaaa aaaggacaca acacctggga taacaatctc gatcttggat
     1801 ttggtttagt gcgtcaggga ttggcagatg tgcgcaagag cgacgaccgt atcgattttt
     1861 ccagcaagtt tggtaaacag gcattcggaa aacagaattt gtattatgca acactcttca
     1921 actttcgtag tcagtttgcg ccgggataca attatcccaa cgactcggtg gtgatctccc
     1981 gctttttggc tccggggtat tttacactgg cacttggtct cgattacaag cccgtaaaaa
     2041 acatggccat cttcgttgct ccactcaccg gaaggatcac cgtagtgaac gatcaactgc
     2101 tggcagataa cggcgcattc ggtgtggaga aagcaaccta cgatacggct ggtgtaaaaa
     2161 ttaccaacgg aaaacgcacc ttttcggagt ttggtggtta cctcaaattc acctacaaat
     2221 ttgatatcgg aaaatccacc acatatagta cacgtcttga tatcttttcc aattacctgc
     2281 gcaatccgca aaacctcgat atcatgtggg acaataacct cgtgattaaa gtgagcaaat
     2341 acattggggt aaccttttct gcgttgatga tttacgacga cgataccaat attgccgttg
     2401 ataccaacag cgatggagta gtggacggtg aaggtcctcg cttgcagttt cgtcagattt
     2461 tcggagtagg attctcatac aaattctgat gaaaaaaaac tggctcttac ctgtcattgc
     2521 attattgctc atcaccggtg tgggaatagc cattgccgct tacatgaaca gtaaggccta
     2581 cgaagaggca gatgtattgc cggtctataa tccctcgcag gtgaacgatt cgctggttga
     2641 tccaaccatg cacaatgtgg gaatgagaca tcgtgtgggt gatttttcat tgtacgatca
     2701 gcgcggcaga accatcacgc aaaaagacct ggaaggaaaa atttatgtcg ccgatttttt
     2761 ctttaccacc tgcaaaagta tttgtccgaa aatgacatcg caactgcagc gtgtacaaga
     2821 gaaatacaaa agcaatgcac aggtgatgat tctttcgcat accgttttac cagaggtaga
     2881 tagtgttgag acgatgatga actacgccat gcagtacggt gcggataacg ataaatggtt
     2941 gtttcttacc ggggaaaaat cgatgattta ttcaatggcg cggaaaaatt atttcatcgt
     3001 taaagaagcc gaacaaggca gtggcgacgg tgatggggat tttattcaca ccgaaaattt
     3061 tgtactcatc gataccaaaa aacgcatccg cggatattac gatggcacct ccaaagaaga
     3121 tgtagatcgc ttgctgggag atatggataa gttgttgcgg gaggaaggaa ttaaataatc
     3181 cacctacgct aaggcttcgg tggcataaat gtgcgaatgt gataatttgc gaatgctgaa
     3241 gacggaagtc gtattcctca ctttaagtaa tggttgctaa ggcttaactt ttacatctaa
     3301 ccaagaactt ctgattgaag cagatgggca cttcgatagg gcagtggggg gcaggaagga
     3361 tgggagacga atgaaacaaa ccaaaggcta ttattgtatt taaccgcatt acacagggga
     3421 aatttctgat agaattttgt tttttggcgg gcttgagcta tcttaatccc atgaaggaaa
     3481 ttacagaagc gttagtcaaa tttcgtaatg aaagagattg ggaacaattc cacaatccga
     3541 aagatctggc agttgcttta aatattgaat ctgctgagct tctggagctt tttctttgga
     3601 aaaaaccgga agaagccaat tcagaaaaaa tcaaagagga acttgctgat gtattcgctt
     3661 atgctttttt gcttgcggag aaatacaaac ttgatgtgaa agatattgtg ttggagaaaa
     3721 taaaaaagaa tggagagaaa taccctgttg acaaagcaaa gggatctgct aaaaagtatg
     3781 atgagttatg agtatgaatt tcgatctttc aatagagatt tcggaggaat acgattttag
     3841 cagtggtgga ttgtcgaaaa tagaacaaaa cccatgggtt aaaaatcaat ggcctttggt
     3901 ttattttatt cagaatcgca gtgaaaaact ggcatatgtt ggtgagtcta ccaatgcttc
     3961 aagtcgattt aaaaatcact tatcaaatcc gcagcgatta aagcttaata agatctcgat
     4021 tataggatca gataaattca acaagtctgc aacactagat cttgaggcaa atttgattca
     4081 gtatttaacc gctgagggga gttatagttt gcaaaatggt aatcatggct taattaacca
     4141 taattattat cagcaggatt tatataaaaa tttgtttcat gaagtctgga aaaagcttct
     4201 ggaaaaaaag attgttacga agacattgtc tgagatcgaa aactcag
//
LOCUS       DAOMYG010000287         4233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_7374_length_4233_cov_7.492101, whole genome shotgun sequence.
ACCESSION   DAOMYG010000287 DAOMYG010000000
VERSION     DAOMYG010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7374_length_4233_cov_7.492101"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1541
                     /locus_tag="PK610_09555"
     CDS             <1..1541
                     /locus_tag="PK610_09555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRJ36223.1"
                     /translation="KELNLLYVDEPKPGELMKTGIDAMLQSLDPYTVYYPESDIEDYR
                     FMTTGQYGGIGSLIRKSGEYVQITDPYEGYPAHKAGLIAGDVIVEVDGKSVKGMDSDE
                     VSKVLKGAPGTKVKIKVKREGESSELERTLTREEIKIKDVPYFGMIDAKTGYIKLTGF
                     TQSASSEVKAAFTDLKGKGMKQLVFDLRGNGGGLLTEAVNIVGVFVPKGTVIVKQKGR
                     APNTNYTYPTKDSPLDTEIPLVVLVDEGSASASEIVSGAIQDLDRGVVIGQRSFGKGL
                     VQQTRNLPYNTMVKLTISKYMTPSGRCVQKLDYSNKDKGGKATVMADSLLKKFKTKNG
                     RDVFEGRGVDPDVMVNRKTYSRIATVLYTQNIIFDFATKFHRENKSVATARDFRVTDN
                     IYQEFLKFASAKDFTYKTASEEKFEELKKTAEREKYFTGAESEFAALLKKLQPNKEDD
                     LMRFKDEIIDFLELEIVGRYYYQNGKIEHSLRADEEIQEALTLFSDPARYSSILSGKN
                     TGKK"
     gene            1545..3104
                     /locus_tag="PK610_09560"
     CDS             1545..3104
                     /locus_tag="PK610_09560"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRJ36224.1"
                     /translation="MILQRYASQLQFLALLLLGVGLSTSVFLMSVGGILFSIAWISFP
                     DLKTRIAKGIRSPYVILSAALFLLHLVGLLWTEDYKYAFNDIRIKIPLLLFPLFFASS
                     PQPEPKKLKLLLALIVAATLISTLISFGIYLKWLPVSKDISDIRNISPFVSHIRLSLV
                     VCASVVLLWWLRLKHRMIPAFTAVVITLWFIYFLYLIESATGIFILCSTALLFGSILA
                     YQSSQRWIKIGIPVVLLAGLIFIFLSIQKSILELRRPKEDPQTFVLQSPDGCYYTHYR
                     DNTMLENGYYTWSHLCLQELEQAWNERSKISFDGNDNKGQRIYGTIVRYLTSKGLHKD
                     RKGVYALDNEDIHHIENGIANVNYVHKSGLKRRLEEIIFEIDSYFTNNYYTGNSLTQR
                     FEFWSTGIGIFRENIWAGVGTGDAQLAFDRAYEKKESPLPKNNRLRSHNQFIAMAVAF
                     GLPGIVLFILFAFYPFSRAIKENNIIFLLFYSGALISFFTEDTLETQAGITYFCFFTG
                     LLSYFSETKKI"
     gene            complement(3101..4144)
                     /locus_tag="PK610_09565"
     CDS             complement(3101..4144)
                     /locus_tag="PK610_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRJ36225.1"
                     /translation="MKKVLLEMEKLRYPSTGLGQFCLHLGKSMAKEDNKGLDLHFYLP
                     ENRVGEFGNQFHYHIRKPWHKVFIPGSSGFDVWHCMHQDSAYLPSGNAKLLLTVHDLN
                     FMQKYTGAKLKARFQRLQKKVDRSSGIAVISEYTGEILKENIKIPDIPVKVIYNGNPL
                     QQFSEAKQPVFIPDRKFFLAIGVLSPRKNFHVLPPLLKKFPDHFLVVAGPEHSTYTKT
                     IIEAAEYWGVKDRLIMCGSVNDEQKYWLYKNMEALLFPSLSEGFGLPVIEAMSLGKPV
                     FLSRLTSLPEIGGDQAFYFDDFSPECIQNTVESGLQFYTSQPEIPNALIQHAAKFSWE
                     SAAKEYLKFYIEI"
BASE COUNT         1284 a          884 c          842 g         1223 t
ORIGIN      
        1 acaaagagct gaacctgctt tatgtcgacg aacccaaacc gggtgaactg atgaaaacag
       61 ggatcgatgc catgcttcaa tcactcgatc cttacacggt atactatccc gaatcggata
      121 ttgaggatta tcggtttatg actaccggtc agtacggagg aatcggatca ctcattcgga
      181 aatcgggcga gtatgttcag attacggatc cttatgaagg ttaccctgcg cacaaagcag
      241 gattgattgc cggagatgtt attgtggaag tggacggaaa gtcggttaaa ggaatggact
      301 cggatgaagt gagtaaagtt ttgaaaggcg cacccggcac caaagtgaaa attaaagtaa
      361 aacgtgaagg cgaatcatcc gaacttgaac gaacgcttac ccgcgaagaa atcaaaatca
      421 aagacgttcc ctatttcggg atgattgatg caaagaccgg ctatattaag cttacaggtt
      481 ttactcaatc tgcaagctcc gaagtaaaag cagcattcac cgatctaaag ggaaaaggaa
      541 tgaagcagtt ggtatttgat ttacgcggta acggaggagg tcttcttaca gaagcggtga
      601 acatagtagg tgtatttgta ccaaaaggca cagtaatcgt taaacaaaaa ggaagagctc
      661 ccaatacgaa ttatacgtat ccaactaaag acagtccgct tgacactgaa ataccattgg
      721 tagttcttgt tgatgaagga agcgcctccg catcagaaat tgtttcagga gcgatccagg
      781 atctcgaccg aggggttgtg attggtcaac gttccttcgg caagggattg gtacaacaaa
      841 caagaaatct tccgtataac actatggtga agctaaccat ttccaaatac atgactccaa
      901 gcggacgatg cgttcaaaag ctggattact ccaacaagga caaaggcgga aaagctacgg
      961 taatggccga ctcgcttttg aaaaaattca aaacaaaaaa tggtcgcgat gtttttgaag
     1021 gccgcggagt agatcctgat gtaatggtga accgaaaaac ctactccaga attgcgactg
     1081 ttctgtacac tcagaatatc atttttgatt ttgcaacgaa attccataga gagaacaaat
     1141 cggttgccac tgcgcgtgac tttagggtga ccgacaacat ctaccaggaa tttttgaagt
     1201 ttgcatccgc aaaggatttc acttacaaaa cagcaagcga agaaaaattt gaagagctca
     1261 aaaagacagc tgagcgtgag aaatatttca ccggtgccga gtctgaattt gccgctttgc
     1321 taaaaaaact acaacccaat aaggaagacg atcttatgcg tttcaaggat gagatcattg
     1381 attttcttga attagagatt gtggggcgtt attattatca aaacggaaaa atagaacatt
     1441 cgctgcgtgc cgatgaagaa attcaggaag cgctcacact attttccgat cctgctcgtt
     1501 attcatctat tcttagcgga aaaaacaccg gtaagaaata aaaggtgatc ctacaacgct
     1561 acgcatccca acttcagttt cttgcactcc tgctcctggg tgttggattg agtacttctg
     1621 ttttcctgat gagtgttgga ggcatattgt tttcaattgc atggatttct tttcctgatt
     1681 taaaaacccg gatcgctaaa ggaattcgtt ctccttatgt catcttatcc gcagcattgt
     1741 tcctgcttca tttggttgga ttgttatgga cagaagacta taaatacgcc ttcaatgaca
     1801 tccgaattaa aatcccactc ctccttttcc ctttgttttt tgcttcttcc ccacaacctg
     1861 aaccgaagaa gttaaaactt ctgcttgcgc ttatagttgc tgcaacacta atttccacct
     1921 tgatctcctt cggtatttat cttaagtggt taccggtaag caaagacatc agtgatatcc
     1981 gaaatatttc tccttttgtt tcccacatcc gcttatctct ggttgtttgc gctagcgttg
     2041 tattgttgtg gtggctgcgt ttaaaacatc ggatgattcc agcattcact gcggttgtca
     2101 ttactttatg gttcatctat tttctctacc tgattgaatc ggcaacagga atatttatat
     2161 tatgcagtac agcacttctc tttggctcta tcctagccta tcaatcctct cagcgatgga
     2221 ttaaaattgg aattcctgtt gtgctattgg cagggctaat ctttattttc ctatccattc
     2281 aaaaaagcat tcttgaactg cgtagaccaa aagaagaccc acaaactttt gtattacaat
     2341 cgcctgacgg atgttactac acccattacc gcgataatac tatgcttgaa aacggttatt
     2401 atacctggag tcatttatgt ttacaggaac tcgagcaagc atggaatgaa agatccaaga
     2461 tctcattcga cggtaatgac aataaaggtc agcggattta cggaactatc gttcgatact
     2521 taacaagcaa gggtttacac aaggacagaa aaggggtgta tgctttagac aatgaagata
     2581 ttcatcatat cgaaaatggt attgccaacg ttaattacgt tcacaaaagc ggattaaagc
     2641 gcaggctgga agaaattatt tttgagatag attcctactt caccaataat tattacaccg
     2701 gaaattcgct aacacaacga tttgaatttt ggtcgaccgg tattggtatt ttccgtgaaa
     2761 acatttgggc aggagttggc accggtgatg cacaattggc ctttgatcga gcttacgaaa
     2821 agaaagaatc tccacttcca aaaaacaacc gattacgttc ccataatcaa tttattgcaa
     2881 tggcagttgc tttcggatta cccggaattg ttcttttcat tctctttgca ttctaccctt
     2941 tttcaagggc tataaaagaa aacaatatca tctttcttct attctattca ggcgcattga
     3001 tttccttttt cacagaagac actcttgaaa cacaggcagg catcacctat ttctgttttt
     3061 ttacaggact actctcctac ttctctgaaa caaaaaaaat ctagatctct atgtagaatt
     3121 tcaaatattc ttttgctgca ctttcccatg aaaacttcgc tgcatgttga ataagagcgt
     3181 tgggaatttc cggttgtgac gtatagaatt gcagaccact ctctacagta ttttgaatgc
     3241 attccggaga aaaatcatca aaataaaaag cctgatcacc accaatttcc ggtaagctgg
     3301 ttaggcgtga taaaaaaaca ggcttcccca agctcattgc ttctatcact ggcaatccaa
     3361 atccttcgga aagcgatgga aacaataaag cttccatatt tttatagagc caatattttt
     3421 gttcatcgtt caccgatccg cacatgatga gtcggtcttt aactccccaa tattctgcag
     3481 cttcaataat agtctttgta taggtagaat gttcaggacc cgcaacaacg agaaaatgat
     3541 ccggaaattt tttcaatagc ggtggtaaaa catgaaaatt ctttcgagga gaaagcaccc
     3601 caattgccag gaagaatttt cgatctggaa taaatactgg ttgcttcgcc tctgaaaact
     3661 gctgcagtgg attaccattg tagatcacct ttacaggaat atcaggaatt ttgatgtttt
     3721 cttttaaaat ctcgcctgta tattcagaga taacagcaat acctgaagat cgatctactt
     3781 tcttttgcaa acgttggaac cgtgccttca attttgctcc ggtatatttt tgcataaaat
     3841 ttagatcgtg taccgtaagc aaaagttttg cgttaccgga cggcaggtag gcagaatcct
     3901 ggtgcataca gtgccaaaca tcaaacccgg aagaacccgg aataaagact ttatgccagg
     3961 gtttcctaat atgataatga aactgatttc caaattcgcc cacacgattt tccggaagat
     4021 aaaaatgaag atcaagtccc ttattatctt ccttcgccat ggatttaccc aaatgcaaac
     4081 aaaactgccc taaccctgta gagggataac gcaatttttc catttcaagc aaaacctttt
     4141 tcatttagaa gaattataac gctcacgcaa ttttctatac ttcattcgtg ttgctgcagc
     4201 cgatatccgt gcaatacgaa atccggcttt tcc
//
LOCUS       DAOMYG010000288         4227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_7393_length_4227_cov_9.361218, whole genome shotgun sequence.
ACCESSION   DAOMYG010000288 DAOMYG010000000
VERSION     DAOMYG010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7393_length_4227_cov_9.361218"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1395)
                     /gene="murC"
                     /locus_tag="PK610_09570"
     CDS             complement(40..1395)
                     /gene="murC"
                     /locus_tag="PK610_09570"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRJ36226.1"
                     /translation="MNLSGLRHIYFLGIGGIGMSALARYFLGRGVRVSGYDKTETDLT
                     AQLVKEGMQIHYHDAPDQLPPDIDLVILTPAIPKDLQEWKRLMEKKIPIYKRAEVLGM
                     ISKDHTCLCVAGTHGKTTTTTLLTHILKCSGVNVTAFLGGIAGNYGSNFISGNSEIVV
                     MEADEYDRSFLQLSRNGIIVTSSDPDHLDIYGTAENMLLAYSDFINGVEKNGIVVLRS
                     GLPLTLNANVQGYADQIYYSDQSNYRAENIRVENGLFVFDYVGEKISMTGLEFTMPGR
                     HNVENATAAITLALSFGATEKGVRLALKDFKGIRRRFEFVLRSEKSVLIDDYAHHPAE
                     LKAAIGAARELFPTKRITGLFQPHLFSRTRDFMEGFAESLSMLDELILLDIYPARELP
                     MEGVSSSALLEQIKTPKRELLSKEDAIKELVNRKPEVLLILGAGDIDLLVEPIRKGLE
                     G"
     gene            complement(1392..2513)
                     /gene="murG"
                     /locus_tag="PK610_09575"
     CDS             complement(1392..2513)
                     /gene="murG"
                     /locus_tag="PK610_09575"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRJ36227.1"
                     /translation="MRRKEDLMPLRKMLISGGGTGGHIFPAIAIANAVKKRYPDVEIL
                     FVGAEGRMEMEKVPAAGYKIAGLRIQGMPRKVTLKWFSFFRDLLRSMVKARRIMKEFN
                     PDVVVGVGGYASGPMLKIATQKKIPTVIQEQNSFPGKTNKMLAKSVSRICVAYDGLEK
                     WFPKDKIVLTGNPVREDVVQLEGKRARAAELFGLNAEIKTILIVGGSLGARTINEAVH
                     YSIERFKAAGVQVVWQTGKSYFPVAQKAVQDAQAENIKVMDFITRMDLGYAIADVVIS
                     RAGAIAISELCLVRKPVILVPSPNVAEDHQTKNALALVNHHAALLVKDADAKEQLATT
                     VLELLENEEKCHSLHRNIGGLGFTNSAEAIASVIADLVK"
     gene            complement(2479..3672)
                     /locus_tag="PK610_09580"
     CDS             complement(2479..3672)
                     /locus_tag="PK610_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRJ36228.1"
                     /translation="MRTFFTYLKGDKVIWMITLILSLFSLVSVYSFTPILARGDSGTE
                     GFLLKHVMMVFSGFVIMFYVHNINFKYYSRISQILIWVSVILLFVTLVAGVRINAAER
                     WLEIPVLNIKFQTSDFAKVVLIVFVARMLTINRDKFHDFREGIVPILIPVGLVIGLIV
                     TQNLSTAVLIFGLTLILFFIAKVPFKYIFGIVGGAIAAFGLMILLAKANPDLLPRLET
                     WMNRFTSHSSEDPRKQWQINNAMMAIDNGGLLGKGPGNGQMKHIIPEAYADFVYASFI
                     EEFGSMGGGVLLLMYMMLLFRGIRIASKCESVFGSLMVIGLCLNLVFMAFINMAVCTK
                     IIPVTGQNMPLISMGGTSTWFTFISLGIILSVSRSVEEEVIKQEGNTVRTDLNEEKGG
                     SYAVA"
     gene            complement(3673..4170)
                     /locus_tag="PK610_09585"
     CDS             complement(3673..4170)
                     /locus_tag="PK610_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36229.1"
                     /translation="MEKRSTQTRTGSWLDTRSKGVSLIEHRNESVAVIDGVEYISDSK
                     ATDPRAVEETLMQIEKPVTLIMQISDTHENYSCLYDVVKYKVVSLVVFGTGSEELIRN
                     SIGQNVDHFIRANDLQDAVSQSKSLTKTGGVVVFSPGCTSFSLFDDYRHRGDSFKAFV
                     GGIFK"
BASE COUNT         1268 a         1020 c          736 g         1203 t
ORIGIN      
        1 ttggaataaa aatccaaaat gctatgttga gaatacgctt catccttcca accctttccg
       61 tattggttca acaagaagat cgatatctcc ggcacccagt atcaataaga cttccggctt
      121 tctgttcact aattctttta ttgcatcttc cttggaaagg agctctcgtt ttggtgtttt
      181 aatctgttcc aaaagagcag aagaacttac tccttccata ggcaattccc gcgcaggata
      241 tatatccagc aatatcaatt catccagcat tgacaaactc tcagcgaaac cttccatgaa
      301 atcacgcgtc ctgctgaata aatgcggctg gaataatccg gttattcttt ttgtcggaaa
      361 aagttcacgg gcagcaccaa ttgcagcctt taactccgct gggtggtgag cataatcgtc
      421 aataaggaca cttttttcac tgcggagaac aaattcaaag cgacgacgaa tgcctttaaa
      481 atctttcaat gcaagacgca cgcctttttc tgttgcccca aatgaaagtg caagtgtaat
      541 tgctgcagtg gcgttttcga cattatgtct tcccggcatt gtaaactcaa gtccggtcat
      601 ggaaattttt tcacctacat agtcaaatac aaacaagccg ttttcgaccc tgatattttc
      661 agcgcgataa ttggattgat cactgtaata aatctgatct gcatacccct gtacatttgc
      721 attcagcgtc agagggagtc cgctgcgcaa taccacaatt ccattcttct ctactccatt
      781 aataaaatcg ctgtaggcaa gcagcatatt ttctgctgta ccatatatat cgagatgatc
      841 gggatcagag gaggttacaa ttattccgtt ccttgataat tgcagaaagg aacgatcgta
      901 ttcatctgct tccatcacaa cgatttcaga attgccggaa ataaaattgc ttccgtaatt
      961 ccctgcaatt ccaccaagaa atgctgttac attaacaccc gaacatttga gaatatgtgt
     1021 gagcaaagtt gtggtagtcg tttttccatg agtaccggca acacataaac aagtatggtc
     1081 cttactgatc attcccaata cttcggcacg cttataaatc ggaattttct tttccatcag
     1141 acgtttccac tcctgaagat cttttggaat agcaggagtg agaattacca aatcaatatc
     1201 gggaggtaat tgatcgggag catcatggta atgaatctgc attccctcct ttacaagttg
     1261 tgcggttaaa tcagtttccg ttttatcata acctgaaacg cgaactcctc ttcccagaaa
     1321 atagcgtgca agcgcactca taccgatacc accgatacca agaaaataga tatgtcgcag
     1381 tccactcaag ttcatttcac aagatctgct atgaccgaag caatagcttc tgccgaattc
     1441 gtaaaaccca aaccaccgat attccgatgc agagaatggc atttttcttc gttctccaac
     1501 aattccaaaa cagttgttgc cagttgctct ttcgcatcag catcttttac aagcaatgca
     1561 gcatgatgat taaccaaggc caatgcgttt tttgtctgat ggtcttctgc aacgtttgga
     1621 gatggaacca gaattacagg tttgcgcaca aggcacaatt ccgaaattgc gattgcccct
     1681 gcacgagaaa tcaccacatc tgctattgca tatcccaaat ccattctggt gataaaatcc
     1741 atcaccttaa tattttctgc ctgtgcatcc tgaaccgcct tttgtgctac aggaaaataa
     1801 cttttaccgg tttgccaaac cacttgcact cctgctgctt taaatcgctc gatggaataa
     1861 tgaacggctt catttatcgt tcttgcacct aaactaccgc ctacaatcag aatagttttt
     1921 atctcggcat tcaatccaaa aagttcggca gctcttgccc ttttcccttc taattgaacc
     1981 acatcttccc gcaccggatt tccggtcaat acgatcttat cttttggaaa ccacttttcc
     2041 aaaccatcat aggcaacaca aatcctgctg accgatttag caagcatctt atttgtttta
     2101 ccgggaaatg aattctgttc ctgtatgacg gtaggaattt tcttttgcgt tgcaattttc
     2161 aacatcggtc cgctggcata tccacctaca ccaacaacaa catcaggatt aaattccttc
     2221 atgatgcggc gcgccttcac catacttcgc aatagatccc tgaaaaagga aaaccattta
     2281 agcgtaactt ttcttggcat tccctgaatt cgtagtcctg caattttgta tcccgcagca
     2341 ggaacttttt ccatttccat tcggccttct gcacccacaa aaagaatttc cacatccgga
     2401 tagcgttttt tcactgcatt ggcaattgca attgcaggga aaatatgtcc accggttcca
     2461 ccaccactta tgagcatttt acgcaacggc ataagatcct cctttctcct cattcaaatc
     2521 agttctaaca gtatttcctt cctgttttat tacttcctct tcaacagatc ggcttacact
     2581 taatattatt ccgagtgaaa taaaagtaaa ccaggtcgaa gtaccaccca tggatatcaa
     2641 aggcatattt tgacctgtaa cgggaattat tttcgtgcat accgccatat tgataaatgc
     2701 catgaaaaca agattcagac ataaaccaat caccatcaat gaaccaaaaa cactctcaca
     2761 tttcgatgcg atccgaattc ctctgaacaa aagcatcatg tacatcagca acaatactcc
     2821 acctcccatc gatccgaatt cttcaataaa agaggcataa acaaaatctg cgtaagcctc
     2881 aggaatgata tgtttcatct gaccattacc cggacctttt ccaagtaaac caccattatc
     2941 aattgccatc atcgcattat tgatctgcca ttgtttacgc ggatcttctg aagagtggct
     3001 ggtaaatcga ttcatccacg tctccaatcg gggtagcaga tcaggatttg ctttcgctaa
     3061 aaggatcatc agtccgaacg cagcaattgc accaccaaca ataccaaaaa tatatttaaa
     3121 cggaacttta gcgataaaaa acaaaatgag cgtaagtcca aaaatgagaa ctgctgtaga
     3181 aagattctgt gtaacaatta atccaataac caatccaacc ggaattaaaa tcggcacaat
     3241 tccctctctg aaatcgtgaa acttatctct gttgatggtg agcattcgcg ccacgaaaac
     3301 aatcagcact acttttgcaa aatcggatgt ttggaatttg atattcagta caggaatttc
     3361 cagccaacgc tcagcagcat tgatccggac acctgcaacc agggtaacaa aaagtaaaat
     3421 cacacttacc cagatcagga tctgagagat gcgcgaatag tatttaaagt tgatgttgtg
     3481 cacataaaac ataatgacga atccggagaa taccatcatc acatgcttca acaggaagcc
     3541 ctcagttcct gaatcaccac gagcaaggat gggagtaaag gaatataccg acactaacga
     3601 aaacaacgaa agtatcaggg taatcatcca gatcacctta tcgcctttga gatatgtaaa
     3661 aaacgtacgc acttacttaa aaatacctcc tacaaaagct ttgaatgaat cgccacggtg
     3721 acggtaatca tcaaagagtg aaaaacttgt acaacccgga gagaacacca ctacccctcc
     3781 ggtttttgtg agtgattttg attgtgaaac ggcatcctga agatcatttg cccttataaa
     3841 atgatcgaca ttctgtccaa ttgaattcct gatcaactct tcgctgcctg taccaaacac
     3901 cacaagtgaa acaactttgt atttcactac atcgtacaga caagaataat tttcatgcgt
     3961 atcagagatc tgcatgatca gcgtaacagg tttttcaatt tgcattaatg tttcttccac
     4021 cgcacgcgga tctgttgctt tggaatcgct gatgtattct acaccatcaa taactgcaac
     4081 agactcgtta cggtgttcaa ttaacgaaac acctttggag cgggtatcca gccagctccc
     4141 tgttcgggtt tgtgttgaac gtttttccat aacggaagta ttaaagcgcc tttacagctt
     4201 gtttaaattt ttgtccacgc tcttcgt
//
LOCUS       DAOMYG010000289         4212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_7442_length_4212_cov_6.885013, whole genome shotgun sequence.
ACCESSION   DAOMYG010000289 DAOMYG010000000
VERSION     DAOMYG010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7442_length_4212_cov_6.885013"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..597
                     /locus_tag="PK610_09590"
     CDS             46..597
                     /locus_tag="PK610_09590"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRJ36230.1"
                     /translation="MLRRKSGKVFHFKQDVAKLKGLSDSLFERIAPGIALPDSPVLNE
                     EKKVISEVKKVDINAADTSEWIALPGIGPAFAKRIVAYRERLGGFHSAVQLLEVYGIT
                     DEKWNAIKDKLEVKTLLSPTDLNTCTLDQLLCFPYLKPVVARGIINYREKHGSYKRIE
                     DLLNTDLVDAELYAKIAPYFFVK"
     gene            594..1127
                     /locus_tag="PK610_09595"
     CDS             594..1127
                     /locus_tag="PK610_09595"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRJ36231.1"
                     /translation="MSQLQDRIRASIRDVKDFPKPGILFKDIMPVLEDPELSADIVKH
                     FASVFSSQGINAVVGVESRGFLFGMALAIQMQVPFVSVRKKGKLPYQTISYKYDLEYG
                     SAEVEMHIDSIKAGWKVLIHDDLLATGGTATAAAELVKMQGGNVEAFAFLVELEFLNG
                     RERLEPYTKNIVNLVAY"
     gene            1134..2276
                     /locus_tag="PK610_09600"
     CDS             1134..2276
                     /locus_tag="PK610_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRJ36232.1"
                     /translation="MEFAKTENQNMIAQMVRDFAEKEIRPNVMDWDESQTFPRELFRK
                     AGELGIMGVLVPQEYGGSGLSYFEYISVIEEVAKVCGSVGLSVAAHNSLCTNHILEFG
                     NEEQKKKYLPKLASGEWIGAWGLTEANTGSDAMRMKCVGKQDGDHWVINGTKNWITHG
                     ISAEVAVVLVRTGELLDSKGITAFVIERGTPGFSGGKKENKLGMRCSETAELIFDNCR
                     VHKSQILGEVGMGFVQAMKILDGGRISIGALALGIAKGAYEASLKYSKERQQFGQPIA
                     NFQAIGFKLADMATEIQAAELLLYQAADLKNRKQKVTREGAIAKYYASEVSVRVSTEA
                     VQIFGGYGYTKDFPVEKFYRDSKLCTIGEGTSEIQKLVISREILKA"
     gene            2332..2523
                     /gene="rpsU"
                     /locus_tag="PK610_09605"
     CDS             2332..2523
                     /gene="rpsU"
                     /locus_tag="PK610_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRJ36233.1"
                     /translation="MLIIPVKEGESIEKALKKYKKKYEKVGVVKQLRERQSFTKPSIN
                     RRQEIIRAKYRLRMRLAEE"
     gene            2583..3464
                     /locus_tag="PK610_09610"
     CDS             2583..3464
                     /locus_tag="PK610_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRJ36234.1"
                     /translation="MTDSSFIAYLSDEKRYSQHTVRAYRDDLNEFSAFCKRVFEIEDP
                     SVSDHQIVRSWVVELMQEGKNPRTIRRKLSCLNAWFRFLMTTGKVSSNPVRKVVSPKI
                     AKRLPVFVEESRIESLFSATIFPDTNVGIRDRLIMELLYGTGMRRAELVGLKIGDVRG
                     DAIRVLGKRNKERIIPITVPLKELITQYLPVRTEISQGKESEILLLDDKGNNISPEFV
                     YKKVNYYLQQVTSLEKRSPHVLRHTFATHLLNQGADLNAIKELLGHSSLAATQVYTHN
                     TIEKLKSIYNQAHPLAG"
     gene            3484..3786
                     /locus_tag="PK610_09615"
     CDS             3484..3786
                     /locus_tag="PK610_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HRJ36235.1"
                     /translation="MQIQIHSIHFDADQKLIDFCHKKMEKLSTVHEKITTTEIFLRLD
                     KDTTNENKVAEIKMKVPGKELFASRQCKSFEEAVDLSVEALKNQMEKYKGKTLGKL"
     gene            3958..4030
                     /locus_tag="PK610_09620"
     tRNA            3958..4030
                     /locus_tag="PK610_09620"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3991..3993,aa:Thr,seq:tgt)
     gene            4062..4142
                     /locus_tag="PK610_09625"
     tRNA            4062..4142
                     /locus_tag="PK610_09625"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4096..4098,aa:Tyr,seq:gta)
BASE COUNT         1229 a          834 c          989 g         1160 t
ORIGIN      
        1 ggtaggattg caccccttaa agtgccctta acgttttctt aagatttgct taggaggaaa
       61 tctggcaaag tttttcattt caaacaggat gttgcaaaac tgaaaggctt atctgattct
      121 cttttcgaaa gaatagcacc cggaatcgct ttacctgatt ctccggtcct aaatgaagaa
      181 aagaaagtga tttcagaagt gaaaaaggta gacatcaatg ctgccgatac cagtgagtgg
      241 attgcattgc ccggaattgg accagcattt gccaaacgaa ttgtcgctta tcgggaacga
      301 ctaggtggtt tccattctgc tgtccagttg cttgaagttt atggaattac agacgaaaag
      361 tggaatgcca ttaaagataa attggaggta aaaaccttgc tttctccaac tgatctcaat
      421 acatgtactc tggatcaatt actgtgcttt ccgtacctta aaccggtggt cgccagaggt
      481 ataatcaact atagggaaaa gcatggttcg tataagcgca tagaagacct gttgaatact
      541 gatttggtgg atgcagaatt atacgctaaa attgcaccct atttttttgt aaagtgagcc
      601 aacttcagga cagaattcga gcaagcatcc gtgatgttaa ggattttccc aaaccgggaa
      661 tcctgttcaa ggatataatg cctgttctgg aagatcctga gctctctgca gatattgtga
      721 agcattttgc ttcggttttt tcctctcagg gaatcaacgc tgttgtcgga gttgaaagca
      781 gaggatttct cttcggaatg gcgcttgcaa ttcagatgca ggttcctttc gtttctgttc
      841 gcaaaaaagg caagctgcct tatcagacca tttcctacaa gtacgatcta gaatacggaa
      901 gcgcagaagt ggaaatgcac atcgattcga ttaaggcagg atggaaagtt ctcattcacg
      961 atgacctgct tgctaccgga ggaactgcta ctgctgctgc tgaactcgtt aaaatgcaag
     1021 ggggaaatgt ggaggcattt gcatttctgg tagaacttga atttctcaat gggcgtgaac
     1081 gccttgaacc atacacgaaa aatattgtta atctcgtagc ttactgattt atcatggaat
     1141 ttgccaagac agaaaaccaa aacatgatcg cacaaatggt gcgtgatttt gccgaaaaag
     1201 aaattcgtcc gaatgtgatg gactgggatg aatcccaaac ttttcctcgt gagctcttcc
     1261 gtaaggcagg tgaactcggt attatgggag ttcttgtacc gcaggaatac ggtggttcgg
     1321 gattgagtta cttcgaatac ataagtgtaa ttgaagaagt agcaaaggtt tgtggttctg
     1381 ttggactttc tgttgctgct cacaattcac tttgtaccaa ccacattctt gagtttggca
     1441 atgaagagca gaagaaaaag tatttaccaa aacttgcatc cggcgaatgg atcggtgcat
     1501 ggggcctaac ggaagcgaac acaggttccg acgcaatgcg gatgaagtgt gttggtaagc
     1561 aagatggaga tcattgggtg atcaacggaa cgaagaactg gattacgcac ggaatttctg
     1621 cagaagttgc agtggttctt gttcgcacag gtgagttact tgattcaaag ggaatcactg
     1681 ctttcgtaat cgaacgcgga actcccggct tcagtggtgg aaaaaaggag aataaactcg
     1741 gtatgcgttg ttctgaaacc gcagaactga tttttgataa ttgccgtgtc cacaaaagcc
     1801 agattttagg tgaagtgggc atgggattcg ttcaggcgat gaaaattctt gatggtggac
     1861 gtatttccat cggtgccctt gcattaggta ttgcaaaagg ggcctatgaa gcatctttga
     1921 aatattccaa agaacgtcag cagtttggtc agcctattgc caattttcag gctattggat
     1981 tcaaactggc ggatatggct accgaaatcc aggcagctga attattgctt taccaggcgg
     2041 cagatttgaa aaaccgcaag caaaaagtta cccgtgaggg ggccatcgca aaatattatg
     2101 catcagaggt gagtgtaagg gtttctacgg aggctgttca gatcttcgga ggatatggtt
     2161 ataccaaaga ttttcccgtt gaaaagttct atcgcgactc caaattgtgc acaattggtg
     2221 agggaacctc tgaaattcag aaacttgtga tatctcgcga aattttaaag gcttgattgg
     2281 atttcaggaa aattccttat ttttgcgacc cattaaaacg aacacacgat tatgttaatc
     2341 attccagtaa aagaaggcga gtcaattgaa aaggcgctga agaagtacaa aaagaagtac
     2401 gaaaaagttg gtgttgttaa gcaacttcgc gagcgtcaat cgttcacaaa gccctcgatc
     2461 aaccgtcgtc aggaaatcat ccgcgccaag tacaggctgc gtatgcgtct tgcagaagag
     2521 taagaaactt ttttctatca tactcgtaaa gggaattcca ttcggattcc cttttcgtat
     2581 ttatgaccga ttcatcattc atcgcctatc tttccgacga gaaacggtac tcgcaacaca
     2641 cagtgcgtgc atacagagat gacctcaatg aattttcagc cttctgcaag cgtgtatttg
     2701 agatcgaaga cccatccgtg tctgatcatc aaattgtgcg gtcctgggta gttgaattga
     2761 tgcaggaagg aaaaaatccc cgcacaatcc gaaggaagtt aagctgcctt aatgcctggt
     2821 tccgcttttt aatgaccaca ggtaaagtat cctcgaaccc ggttcgaaaa gtggtaagcc
     2881 ccaaaattgc aaagcgtctt ccggtatttg tagaagagtc gagaatagaa tcgctttttt
     2941 ccgctaccat tttccctgat accaatgttg gaattcgtga ccgtctcatc atggagctgc
     3001 tttatggaac aggaatgcga agggcggaac tggttggact aaaaataggg gatgtgcgtg
     3061 gcgatgcaat tcgtgttttg ggaaaacgaa ataaggagcg aattattccc atcactgttc
     3121 ctttaaaaga gttaatcaca cagtatttgc cagtgcgtac agaaatttca cagggaaaag
     3181 aatctgaaat acttttattg gatgataaag gcaataatat cagtccggaa tttgtttaca
     3241 aaaaagtaaa ttattacctt cagcaagtca ccagtttgga aaagcggagt ccgcacgtac
     3301 ttcggcatac atttgctacc catttgctca atcagggggc tgatttaaat gccatcaaag
     3361 agttactcgg tcactccagt ttggctgcca cacaggtgta cacgcacaat actattgaga
     3421 aattgaaatc gatttataac caggctcacc ctcttgcagg ctgatccaaa aaaagaaatt
     3481 cttatgcaaa tccagattca ttccattcac tttgatgctg accagaaact gatcgatttt
     3541 tgccacaaga aaatggagaa gctttctacc gttcacgaaa aaattaccac tacagaaatc
     3601 tttcttcgcc tcgataagga tacaacgaat gagaacaagg ttgctgagat caaaatgaaa
     3661 gtgcctggta aagaattgtt cgcaagccgt caatgcaagt catttgaaga agctgtcgac
     3721 ctttcagtgg aagcccttaa gaaccagatg gaaaagtaca agggtaagac ccttggtaaa
     3781 ctataagtgc cggtaaatac ccggttagcg tgccataaat ccccgctacg ccttttgttt
     3841 tcaaaaaaaa ggtgttgatt tttttgcttc atcttgaatg ttttatacat ttgcagacct
     3901 ttttggaaag aggtctgctt tctttattga tgctagcctc tgatacagaa tagattagcc
     3961 ggggtagctc agttggtaga gcagctgatt tgtaatcagc tggtcggggg ttcgagtccc
     4021 ttcctcggct cagttctttg aatgatacat tacaatttgt tggggcgata ccagagtggc
     4081 caaatggggc agactgtaaa tctgctgcgc aagcttcggt ggttcgaatc catctcgccc
     4141 cacaacaaaa aattcaagcg ggagtagctc agttggtaga gcatcagcct tccaagctga
     4201 gggtcgcggg tt
//
LOCUS       DAOMYG010000290         4198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_7488_length_4198_cov_7.458122, whole genome shotgun sequence.
ACCESSION   DAOMYG010000290 DAOMYG010000000
VERSION     DAOMYG010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7488_length_4198_cov_7.458122"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="PK610_09630"
     CDS             complement(<1..1146)
                     /locus_tag="PK610_09630"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRJ36236.1"
                     /translation="MSSALFSDYNISRQLHRSLDDMGFSVPTPIQEKCFSPIMAGRSM
                     VGIAQTGTGKTLAYLLPLFNLWKFTKEPHPTMVVLVPTRELVIQVEEQARKLAAHNAI
                     RIVGIYGGASINTQKEMLAVGVDLLVATPGRLIDLVTTGNLKLKNVKHLVIDEMDEMF
                     GQGFRPQLLRIFDSLPKRRQNLLFSATITDDISEFLDEHYFGLDRVEAGPASAPAGTV
                     EAIAYKVPNFTTKIDLLEHLLRTDGSMRKVLVFVSTIRMAEYLHERLSARIEEMIGML
                     HSNRAQNTRFRLLEEFADGKFKVLIATDLAGRGVDIEDVTHVVNFDLSEQAEDYVHRI
                     GRTGRAGKSGVAISLISQHDRMQDIEDFTGISFQFVPLPDEIEFSPVV"
     gene            1289..2368
                     /locus_tag="PK610_09635"
     CDS             1289..2368
                     /locus_tag="PK610_09635"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRJ36237.1"
                     /translation="MKKSLIVLCSVILVACSKNEKTTTPVVEDITESVYASGSLKTMN
                     QYSVFPVVNGTIKSIHVESGDTISEGDTLFVLANDASRLNTENAQLAADFYDYSSNTS
                     KLDELKSAIEIAKERMNNDSVQYIRQKNLWSQQVGSKSELENRELAYKNSINQYRSAI
                     LRYNDLKKQIQFNSLQSKKNLEISKKGQGDFIVRSEINGKVYSIQKNKGEMVGPQTAL
                     AIVGDANDFIIELQVDEYDIVKIKKGQTVLITMDSYKGESFEAIVHRIDPIMNERTRT
                     FLVEAVFTKRPATLYPHLSLEANIVINTRQAILTIPRECLIDNEYVIKKGGEKVKVVT
                     GLMDYRKVEILSGITEKDLIVIPEE"
     gene            2365..3624
                     /locus_tag="PK610_09640"
     CDS             2365..3624
                     /locus_tag="PK610_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRJ36238.1"
                     /translation="MKFALIFDIARSLMLARFRQTLIAAIGVTFSITMFIALLGFMNG
                     LNSLLDGLILNRTPHIRLYNEIKPNEKQPLLLSEEFRNSYPFIHSIKPSGGRIEIHNA
                     LAIMAELKKDNRILGVSPKIVSQVFFNSGSVDITGVINGVDIDQETRLYAFNDYVIDG
                     KSSDLKLVPNSIILGKAAAQTMMLNIGDLVQVTTPTGERMQLKIVAFFQSGLQELDKT
                     QSYASISTTQKLMGKSNNYISEIQIKLHDMNKAPALAKQFAEIFNVQATDIQTANSQF
                     ETGSSVRSIISYAVGITLLIVAGFGIYNILNMMIYEKLDTIAILKATGFSGKDVNRIF
                     ITIALTIGIFGAVFGLLLGYLSSYGIDQIPFETEALPTIKTYPVAYNISFYVIGGVFA
                     LVTTYLAGFFPARKAGRVDPVVIIRGK"
     gene            3626..>4198
                     /locus_tag="PK610_09645"
     CDS             3626..>4198
                     /locus_tag="PK610_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013551785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ36239.1"
                     /translation="METPALAAKNIVKYFSDPVTVKVLNDVSFSVNRGEFVSVIGKSG
                     CGKSTLLYILSTMDTEYQGELYIDGENMKLKDHSQLADVRNEKIGFVFQFHYLLNEFS
                     VLKNVMLPALKLARLSHEEIEFKAMEKLKLLGIENEARKKANQLSGGQKQRVAIARAL
                     INDPLIIMGDEPTGNLDKKNSDIVFDIFKQL"
BASE COUNT         1355 a          901 c          804 g         1138 t
ORIGIN      
        1 tactactggt gaaaattcaa tttcatccgg caagggtaca aactgaaatg aaatcccggt
       61 aaaatcttca atgtcctgca ttcgatcgtg ctgactgatc aatgaaatgg caacaccact
      121 ttttccggcg cgaccggttc gtccaatacg gtgaacataa tcttcggctt gttcagagag
      181 atcaaaattc accacatggg ttacatcttc aatatccact ccacgacctg ccaaatcagt
      241 ggcaattagc accttgaatt ttccatctgc aaactcttcc aataaacgaa aacgggtatt
      301 ctgtgcacgg ttagagtgaa gcattccaat catctcttca attcgtgcgc tcaaacgttc
      361 atgcaggtat tcggccatgc gaatggtaga tacaaaaacc aacacctttc gcatactgcc
      421 gtcggtacgt aacaagtgtt caagcaaatc tattttcgtg gtaaaattcg ggactttata
      481 cgcaattgcc tcaactgttc ccgcaggagc tgatgcgggg cctgcctcta ccctatccaa
      541 cccaaaataa tgttcatcca gaaattctga aatatcatcc gtaatggttg cagagaaaag
      601 caaattctgc ctgcgctttg gtaatgaatc aaaaatccga agaagttgcg gacgaaatcc
      661 ttgcccgaac atttcatcca tctcgtcaat aacaagatgt tttacattct tcaatttcag
      721 attccctgtc gttaccagat cgatcaatct tccgggtgtg gcaaccaaaa gatcaacacc
      781 aacagccagc atttctttct gagtattgat acttgcacca ccgtaaatac caactatccg
      841 aatagcgttg tgtgcagcaa gcttgcgtgc ctgctcttca acctgaataa ccaactcacg
      901 ggtggggacc aaaacgacca tggtcggatg tggttccttg gtgaacttcc agagattgaa
      961 aaggggtaac aaatatgcca acgttttccc tgtaccggtc tgggcaattc caaccatgct
     1021 tctccctgcc ataataggtg aaaaacattt ctcctggata ggagttggga cagaaaaacc
     1081 catatcatcc aatgaacggt gcagttgacg gctgatattg tagtcggaaa acaaagctga
     1141 agacataata ctgcaaaagt agtcaattcc aaccacagaa cggtaattcc acattaagac
     1201 agcttcagag gctgactttc atcactaaac acaaaaaaac acaaacacag atacctcaaa
     1261 ttgtatattt gacccaatga tgtttacgat gaaaaaaagt ttgattgtat tgtgctctgt
     1321 cattcttgtg gcatgctcca aaaatgaaaa aacaactact ccggttgttg aagacattac
     1381 tgaatctgtt tatgcatcag gctcacttaa aacaatgaat caatactccg ttttccctgt
     1441 ggtgaacgga acaatcaaat cgattcatgt ggaaagcggc gacactatta gcgaaggaga
     1501 tacacttttt gtgcttgcaa atgatgcgtc tcgccttaat acagaaaacg ctcagttggc
     1561 agctgatttt tacgattatt catccaacac ttccaaactc gatgaactga agtctgcaat
     1621 agaaattgca aaagagcgaa tgaataacga ttcagtgcag tatatccgtc aaaaaaatct
     1681 ttggtcacaa caagttggtt ccaaatccga actcgaaaac cgggaactcg cctataaaaa
     1741 ttcaatcaat caataccgat ctgccattct tcgttataat gatctgaaaa aacaaattca
     1801 gttcaactct cttcaaagca aaaaaaatct cgagatcagc aaaaaaggac aaggagattt
     1861 tattgtccgt agcgagatca atgggaaagt ttacagcatt cagaaaaaca agggagaaat
     1921 ggtagggcct caaactgcct tagctattgt tggtgatgcc aatgatttca ttattgaact
     1981 tcaggttgat gaatatgata ttgtaaaaat taaaaaggga caaaccgttt taataacaat
     2041 ggatagttat aagggagagt cgtttgaagc tatagttcac cgcatcgatc cgatcatgaa
     2101 tgaacgcaca cgtacttttt tggttgaggc cgttttcaca aagcgacctg caacacttta
     2161 cccgcatctt tctcttgaag cgaatattgt aattaatacc agacaagcaa tccttaccat
     2221 cccgcgcgaa tgtttgatag acaatgaata cgtgattaaa aaaggcggag aaaaggtaaa
     2281 agttgttaca ggattaatgg attaccgaaa ggtggaaatc ctgagcggca ttacagaaaa
     2341 agacttaatt gttatccctg aggaatgaaa tttgccttga tcttcgatat cgcccgttca
     2401 ttgatgctgg cacgattcag acaaacgctt attgctgcaa ttggtgtcac tttttccatc
     2461 accatgttca ttgcgctttt aggtttcatg aatggcttaa actccctgct cgacggatta
     2521 attctgaaca gaacaccaca cattcgactt tacaatgaaa tcaaacccaa cgaaaaacaa
     2581 ccattgttgt tatctgaaga attcaggaat tcatacccgt ttatccattc cataaaaccc
     2641 agtggaggac gaattgagat tcacaatgca ttggccataa tggctgaatt aaaaaaagac
     2701 aatcgcattt taggtgtaag tccaaaaata gtttcacagg tctttttcaa ctccggatcc
     2761 gttgacatca ccggtgtcat caatggtgtc gacattgatc aggagaccag actatatgca
     2821 ttcaacgatt atgtaatcga tggcaaatca tccgatttaa aactcgttcc aaacagcatc
     2881 attcttggta aagcagctgc acaaaccatg atgctgaata ttggagacct ggtgcaggtt
     2941 accacaccaa caggtgaacg aatgcaatta aaaatcgttg cttttttcca gtccggattg
     3001 caggaattgg ataaaacaca gagttatgcc tccatttcta ccacacagaa attaatgggg
     3061 aaatcgaaca actacatttc cgaaatacag atcaaacttc acgacatgaa caaagcgccc
     3121 gctttggcaa aacaattcgc ggagattttc aatgttcagg caacagacat ccaaactgca
     3181 aattcacaat ttgaaaccgg ttcttcggtt cgttccatta taagctatgc agtgggaata
     3241 accttactta ttgtagcagg cttcggaatt tataatattc tgaacatgat gatctacgaa
     3301 aaactcgata caattgctat tctcaaagca accggatttt ccggaaaaga tgtcaaccgg
     3361 atttttatta caatcgcact taccatcggt atttttggcg ctgttttcgg attattactg
     3421 ggttatttat cgtcctacgg aattgatcag atccccttcg aaacagaggc actaccaact
     3481 attaaaacgt atcccgttgc atacaacatc agtttttatg tgattggcgg agtatttgcc
     3541 ttggtcacca cctatctcgc cggttttttt ccggcacgaa aagcaggcag agtcgatcct
     3601 gttgtaatta tcagaggaaa ataaaatgga aactccggca cttgcagcta aaaatattgt
     3661 gaaatacttc agcgatcctg ttacggtaaa ggtgctgaat gatgtttcct tttccgttaa
     3721 cagaggtgaa ttcgtttccg taatcggaaa atccggttgt ggaaaatcaa ccttgctcta
     3781 tatcctatcc acaatggata ccgaatacca gggcgaatta tacattgatg gggaaaacat
     3841 gaaactaaaa gaccactcac aacttgcaga tgtacgcaat gaaaaaatcg gttttgtctt
     3901 tcagtttcat tatttgctca atgaattcag tgtgctcaaa aatgtaatgc taccggcttt
     3961 aaaacttgct cgattaagcc atgaagaaat agagtttaag gcaatggaaa aacttaaact
     4021 tcttggaata gaaaacgagg cgcgtaaaaa agcaaatcaa ctttcaggcg gacaaaaaca
     4081 acgcgttgcc attgcacgtg cgctcatcaa cgatccgctt atcattatgg gggacgagcc
     4141 aacaggtaat ctcgataaga aaaacagtga tatcgttttc gatatcttta agcaacta
//
LOCUS       DAOMYG010000291         4189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_7519_length_4189_cov_9.011369, whole genome shotgun sequence.
ACCESSION   DAOMYG010000291 DAOMYG010000000
VERSION     DAOMYG010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7519_length_4189_cov_9.011369"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PK610_09650"
     CDS             <1..253
                     /locus_tag="PK610_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ36240.1"
                     /translation="LHFPNPSRENVMLEFDLSSNQSLHFRIVDVSGREIYFDQQSYSQ
                     GRGIKTIQISDWESGVYFVSVYDHNSSVPLVSGLKLFKY"
     gene            complement(308..2275)
                     /gene="ispG"
                     /locus_tag="PK610_09655"
     CDS             complement(308..2275)
                     /gene="ispG"
                     /locus_tag="PK610_09655"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HRJ36241.1"
                     /translation="MQHDLFDYAGFAYCDDLFSYHRFPTREVKIGTTGFGGNNPIRIQ
                     SMTTTDTMDTQGTVEQSIRMIEAGCELVRITAPSKKEAENLSQIKEQLHKRGYNTPLV
                     ADIHFTPNAAEVAARIVEKVRVNPGNYADKKKFEEIEYTDAAYEAELARIKEKFTPLV
                     NICKQNGTAMRIGTNHGSLSDRILSRYGDTPLGMVESAMEFLRICRENDYHDIVISMK
                     ASNAQVMVQAYRLLVAKMKKENMNYPLHLGVTEAGDGEDGRIKSAVGIGTLLEDGLGD
                     TIRVSLTEDPEFEMPVARLLAQRYENRSGHTTIPHHFSVDDLKGKFNPFEYTRRSTHE
                     VINIGARNVPRVIADLSGREKINPASLYPYGYNYSAQLDKWNLSDQACDYVYAGDTSI
                     DFEIPGTLGVILNSSSWKNSSERERTYPLFCGNEFFESEKKHPELNFVAADLATLTVE
                     FISKISNRTDCVLILDTFNEHAMPELRSMFFQLLLSNCNVPCIIKRNYIKLDDERFQL
                     YCATDVGALLLDGFGDGIWVKTVECANPQLINRTVFGTLQATRTRISKTEYISCPSCG
                     RTLFDLQETTQKIRAVTDHLKGVKIGIMGCIVNGPGEMADADYGYVGTGVGKISLYKG
                     KEVVRKNIASDEAVDALIDLIREHGDWVERG"
     gene            2368..2751
                     /locus_tag="PK610_09660"
     CDS             2368..2751
                     /locus_tag="PK610_09660"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRJ36242.1"
                     /translation="MRPLLATLLFLTSFATLHAQEEVFDVVEQKAQFPGGNHALLGFL
                     NQNLQYPEGAKADEAEGKVYVQFVVSSNGKIDPQSIKVYKSVHPLLDKEAIRLVSIMP
                     AWKPAKNNGVPVASRYMLPITFKLL"
     gene            complement(2776..3966)
                     /locus_tag="PK610_09665"
     CDS             complement(2776..3966)
                     /locus_tag="PK610_09665"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRJ36243.1"
                     /translation="MKTVDQFNFAGKRALIRVDFNVPLDAHFHITDDTRIKAAIPTLK
                     KILKDGGAVVLMSHLGRPKNGPEEKFSLKHIVDDLSKKLSVEVKFAGDCIGEEANYLT
                     SQLKPGEVILLENLRFYKEEEKGDVAFAQKLAKHGDVYVNDAFGTAHRAHSSTAIIAQ
                     FFPEAKMFGYVMAGELSSINKVLNNTMRPFTAVMGGAKVSDKIMILEQLINKADHIII
                     GGGMAFTFVKAQGGNVGNSLVENDRLDTAKQILADAAAKGVKIHIPVDAVCADNFSNE
                     ANTQICDIDKIPAGWMGLDISGKTEKNYAEVIAASKTILWNGPMGVFEMSKFENGTKS
                     VAEAIVDATEKGAYSLIGGGDSVAAINKYHLGEKVSYVSTGGGALLEYIESGSLPGVE
                     AVEK"
BASE COUNT         1103 a          946 c          873 g         1267 t
ORIGIN      
        1 attacatttt cccaacccaa gccgggaaaa tgtaatgctt gaattcgatc tttcttccaa
       61 ccaatccctt cattttagaa ttgtagatgt aagtggaagg gaaatatatt tcgatcagca
      121 gagttattcg caggggagag gaataaaaac aattcaaatc tccgattggg aatcgggtgt
      181 ttattttgtt tccgtctatg accacaattc ctctgttccg ctagtaagtg gattgaaatt
      241 attcaagtat tgattcaggg aaaattgcgt tcaaaatgga ttttgaagct tagcgttttt
      301 aatttcatta tccccgctca acccaatctc catgctctcg aatcaggtca atgagggcgt
      361 cgacagcttc atctgaggcg atgtttttac gtacgacttc tttccctttg tacagtgaaa
      421 tttttccaac acctgtgcct acatatccat aatcagcatc agccatttcg cccgggccat
      481 taacgatgca tcccataatg ccgattttaa cgcctttaag gtgatcggta actgctctga
      541 tcttttgggt ggtttcctga agatcgaata atgttcttcc acacgaagga catgaaatat
      601 attcggtttt cgatatgcgt gtacgagtag cctgcagagt accgaaaact gttcggttga
      661 taagttgcgg attagcgcat tcaactgttt ttacccaaat accatcacca aaaccatcaa
      721 gcaataaagc tccaacatct gttgcgcaat acaactggaa gcgctcatcg tcaagtttta
      781 tataattgcg tttgatgata cacggcacat tgcaattgct taacagtaat tgaaaaaaca
      841 tgcttcgcaa ttcaggcatc gcatgttcat taaaggtgtc gagaatgagc acacaatcgg
      901 tgcgattgga aatcttactg atgaattcaa cagtaagtgt tgccaaatct gctgcaacaa
      961 aattcaactc agggtgtttt ttttccgatt caaaaaattc attgccgcaa aagagcgggt
     1021 aagttctttc tctttcactg ctgtttttcc aggaacttga atttaaaata acgcctaatg
     1081 tgcccggaat ttcaaaatca atagaagtat cgcctgcgta tacataatcg catgcctggt
     1141 cactgaggtt ccatttatcg agttgtgcag aatagttata cccatacgga tacaaactcg
     1201 caggattgat cttttctctt ccggaaagat cggcaataac ccggggaaca tttcttgcac
     1261 caatgtttat gacttcatgg gttgaacgac gcgtgtattc gaagggattg aatttaccct
     1321 ttaaatcatc taccgaaaag tggtggggaa tggtagtgtg tccactgcga ttttcgtatc
     1381 gttgtgcaag tagtcgggct accggcattt caaattcagg atcttcagtt agagaaacac
     1441 gaatggtatc accaagcccg tcttcgagca gcgttccgat tcctacagca gattttatac
     1501 gcccatcttc tccgtcgcct gcttcggtta caccaaggtg cagcggatag ttcatgttct
     1561 cctttttcat ttttgccacc agcaaccgat aggcttgcac cattacctgg gcgttcgacg
     1621 ctttcatgga gataacgatg tcgtggtaat cgttctctcg acaaatgcgc agaaattcca
     1681 ttgctgattc aaccattcca agtggtgtgt cgccatacct gctcaggatc ctgtcgctca
     1741 aagaaccgtg attggttcca atgcgcatcg ctgtgccgtt ttgcttgcag atatttacca
     1801 gtggtgtgaa cttttctttt atccttgcca attccgcctc gtaggcagca tcggtgtatt
     1861 cgatctcttc gaattttttc ttgtcggcgt aattaccggg attcaccctc actttttcta
     1921 caattcttgc ggcgacttct gctgcattgg gagtaaaatg aatatctgcc accagcggtg
     1981 tattgtaacc gcgcttatgc agttgttctt tgatctgtga aagattttcg gcctcttttt
     2041 tacttggtgc ggttatcctg acaagctcac atccggcttc aatcatacga atcgattgct
     2101 ccacggttcc ctgcgtgtcc atggtgtcgg tggtggtcat ggattgaata cggatgggat
     2161 tgtttcctcc aaagccggta gtaccaattt tcacctcacg cgttgggaag cgatggtagg
     2221 aaaataggtc gtcgcagtat gcgaatcctg cgtaatcgaa taggtcgtgt tgcatggccg
     2281 gaaagaagga gacaaatata ggcacaacag ggaaacagca attttgttca attcgacgga
     2341 aatcgtattt ttagcaaaac aatgcgaatg agacctttac ttgccaccct cttgttttta
     2401 acaagtttcg ccaccttaca tgcccaggag gaagtgtttg atgtggttga acaaaaagcc
     2461 cagtttccgg gtggtaacca cgccttattg ggcttcctga atcagaattt gcaatatcct
     2521 gaaggagcaa aagcagatga ggcagagggt aaggtttatg ttcagtttgt agtatccagc
     2581 aatggaaaga tcgatcctca atcaataaag gtctacaaaa gcgttcaccc tttattggac
     2641 aaggaagcta ttcgtctggt ttccatcatg cctgcatgga aacctgctaa aaacaacgga
     2701 gtcccggttg cttcacgtta tatgcttcct atcacgttta aattgcttta aatctcctgg
     2761 actatttgct ccgatttatt tctccacagc ttctactccc ggaagacttc cgctttcgat
     2821 gtattccagt aaggcgccgc cgcctgtaga tacataactt actttttctc ccaaatggta
     2881 tttgttaatg gcggcaacgg aatctcctcc accaataagt gaataagctc ctttttcagt
     2941 ggcatcaaca attgcttctg caaccgattt ggtgccgttt tcaaatttgc tcatctcaaa
     3001 aacacccatc ggaccattcc ataaaatagt ttttgatgcg gcgatcactt ctgcgtaatt
     3061 tttttcggtc ttcccggaaa tatccagtcc catccaaccc gcaggaattt tatcgatgtc
     3121 acaaatctgc gtattcgcct cattggagaa attatctgca catacggcat ctaccgggat
     3181 atgaattttc acgcctttag ctgcagcatc ggcaagaatt tgttttgcag tatccagacg
     3241 atcattttcc accagcgagt ttccaacgtt tccgccttgc gcttttacaa atgtaaatgc
     3301 cattcccccg ccaataataa tgtgatcagc cttattgatg agctgttcaa gaatcatgat
     3361 tttatcggaa acttttgctc ctcccattac tgcggtgaac ggacgcatag tgttgttaag
     3421 gactttattt attgaagaaa gttctcctgc cattacatag ccaaacattt ttgcttccgg
     3481 aaaaaattga gcgatgatcg ctgtagaaga atgcgcgcgg tgagcagtgc caaaagcatc
     3541 gtttacgtat acgtctccat gctttgccag tttttgtgcg aacgcaacat cccctttttc
     3601 ttcttctttg taaaagcgca aattttcaag gaggattact tcgcctggct taagttgtga
     3661 ggtaagataa tttgcttctt caccaataca atcgccggca aacttcactt ctacagaaag
     3721 ttttttgctt agatcgtcaa caatatgttt aagtgaaaat ttttcttccg gaccattttt
     3781 cggccttccc agatgcgaca tcagcacaac agcacccccg tctttcagga tttttttcaa
     3841 ggtaggaatg gcagctttaa tgcgagtatc gtcggtgatg tgaaaatgag catcaagcgg
     3901 cacattgaaa tccacgcgga tcaatgcgcg ttttccggca aaattaaatt ggtcaacagt
     3961 cttcattcga ttcggattga ggccccaaaa ataaaggaaa tatttgcccg acgaagatgg
     4021 aattgtcaac aaaaggtatg cgtttgcaac gtgttaaaat agctatcttt aacccgtgaa
     4081 attttcagca ataataggtc agaatgccac caaagaacgc tttctccgtt ccgcccgcga
     4141 tggcagggtt agtcatgctc agctgattgc aggtcctgaa ggctcaggt
//
LOCUS       DAOMYG010000292         4169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_7566_length_4169_cov_13.183520, whole genome shotgun sequence.
ACCESSION   DAOMYG010000292 DAOMYG010000000
VERSION     DAOMYG010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7566_length_4169_cov_13.183520"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /gene="recJ"
                     /locus_tag="PK610_09670"
     CDS             complement(<1..1591)
                     /gene="recJ"
                     /locus_tag="PK610_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020215059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRJ36244.1"
                     /translation="MEKRWVFDESAKVPEKSAAAIRLEQELNLPPALARALVRRGIGD
                     FQEAKCFFRPELENLHDPFLMRDMDKAVERLQQAMSKGENILIYGDYDVDGTTSVALV
                     YSFLASKYHNIDFYIPDRYTEGYGISFKGIDVASDSDVSLIIALDCGIKSNDKIDYAR
                     SKGIDFIICDHHRPGDILPEAAAVLDPKREDCNYPYKELCGCGVGFKLMQALCTVSDY
                     SLEELYNKLDLTAVAIAADIVPITGENRILAYHGLQKINSNPSVWVRAILKLAGKQLP
                     VDISNLVFILAPRINAAGRIDSGRNAVELLIASNEEEAINRGMQLDKHNADRKEFDRN
                     ITREALSIIEENEELKSAKTTVLYRENWHKGVIGIVASRLTESYYRPTILLTLSNGKL
                     TGSARSVRDYDVYEAIEACSHLLEQFGGHKYAAGLTLAEENLDAFRAAFESFVASTIE
                     EHMLIPSIDVEDEIDLEVLRTLPGETLPKFYRILKQFAPFGPGNMNPVFVTHQLRDSG
                     MARTLKEEHLKLQVYSEHDPRL"
     gene            1638..2324
                     /locus_tag="PK610_09675"
     CDS             1638..2324
                     /locus_tag="PK610_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36245.1"
                     /translation="MRNLFLVLFTVGCSLTALAQEDLSFYFADAQPNGGRNLTSFPEA
                     FRGEYISKKDSLLKVTITEDSIHVRLISFVIMTLKEVKDAPGLRLKDSLLFGIYKDRP
                     VEVIRKNDTLIFGMETKYRMFAVSDTSVIRVCGNDLLLSSKDGNGKWTVMRISLIPRD
                     GLKAPGVEFAYVDHDEQIKLIRSQKGLQRKQENGQTFLQLDLAYPDMQRFIEAGGFNM
                     RQSYFRTEYH"
     gene            2321..>4169
                     /locus_tag="PK610_09680"
     CDS             2321..>4169
                     /locus_tag="PK610_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36246.1"
                     /translation="MRKIRDILFHRYSLLAIAVVLVFSGGYLYRSSFISVDGNRLAAN
                     AENVLRESAVYANEWFKSIRVQLLSGQKNAEINSSENELHAVMVFRERQPVFWTNSRI
                     PFDTTALDTLGKSGLIHLRNGWYFYHHEQLDAEHSGIVFTQVRKEYVHKNRYLSDHFH
                     SAWGIPDEVNLVEPGTENSFLVRDENGNAVFALLLSEATITSGSSLYLIAGLLLLGIV
                     LLIRFLKEECENLEKFIGPFYSSLLLVFSVVLLRYWSLSMEFPAAFYAFELFQPDLFA
                     ASFFLPSLGDYLINAFLILYLFYFLDARSSRFNYHLSSNKGAAIGIIAFLLGVLMAFG
                     HSIGFLMQGLIEDSNIEFNINNIFNLSTYSFIGFTIVGLLYFAFYLFCDFTFRVAKKL
                     QIEKLTFLVLLGVFSIFSTFALYLWGVNDPFQIIWPLTILFLLFAVHFVQEKAQLSFN
                     VTVLLLIIFSGFTAHALSKFTGAKERKNRIVLAERLSTDEDPLVEILFSEMEAGLRRS
                     ELLNSAFDSSAVFSKTAFEEQLEKEYFSGDWNAFDIQFYLFAADSTPVGLEGFAPIRE
                     FSDLDNVILENGIPSKFSGNMNFIYNSTNKLSYIIKLPVSARNEAPKGYL"
BASE COUNT         1060 a          876 c          914 g         1319 t
ORIGIN      
        1 gtaagcgggg gtcgtgttct gagtatacct gaagtttgag atgctcttct tttaaagttc
       61 gcgccatacc actgtcgcgg agttggtgtg tgacaaatac cggattcata ttacccggac
      121 caaaaggtgc aaattgtttc agaatcctat agaattttgg caatgtttca cccggtagtg
      181 tacgcaaaac ttccaaatcg atttcgtctt ccacatctat ggatggaatg agcatgtgct
      241 cttcaatggt ggaagcaaca aaggattcaa atgctgcacg aaaggcatca agattttctt
      301 ctgccagtgt taatcccgca gcgtatttat gtcctccaaa ttgttcgagt aaatgactgc
      361 aggcttctat ggcttcgtac acatcgtaat cgcgcaccga tcgtgcagaa cccgtgagtt
      421 ttccattgga aagagtaagc aggatggtag gacgataata tgattctgtg aggcgggatg
      481 caactattcc tataacacct ttatgccagt tttccctgta gagaacagtt gttttagcgc
      541 ttttcagttc ttcattttct tcgatgatgc tgagtgcttc ccgggttatg tttcggtcaa
      601 attctttgcg gtcggcattg tgtttgtcca attgcatgcc gcggttgatg gcttcttctt
      661 catttgaagc gataagcaat tcaactgcat ttctgccgga atcgattctt cctgctgcat
      721 tgattcttgg cgcaagaata aaaaccagat tcgagatatc taccggcaat tgttttcctg
      781 caagtttgag aatggccctt acccataccg atggattgct gttgatcttt tgcaatccgt
      841 gataagcgag gatgcggttt tcaccggtaa tcggcactat atctgcagca atggccaccg
      901 ccgtgagatc aagtttgttg tacagttctt cgagcgaata atcacttacc gtacagagcg
      961 cctgcatcag cttgaaacct acaccgcatc cgcataattc tttataggga taattgcagt
     1021 cctctcgttt gggatcgagc acagcagcag cttccggaag gatatcgccc ggacggtggt
     1081 ggtcgcaaat gataaaatca attcccttgc tcctggcgta atctattttg tcgttgcttt
     1141 tgattccaca atcgagtgca ataatcaagc ttacatcact gtcggatgct acgtcaatcc
     1201 ctttgaagga gattccatac ccttcggtat agcgatcagg aatgtaaaaa tcgatgttgt
     1261 ggtatttact tgccagaaag gaatatacca aagcaacaga agtagttcca tctacatcat
     1321 aatcaccata aatcagaatg ttttctccct tgctcattgc ctgttgcaaa cgttcaacgg
     1381 ccttgtccat atccctcatc aggaatggat catgaagatt ctccagctcc ggccggaaaa
     1441 agcattttgc ttcctgaaaa tcaccaatac ccctacgcac aagcgcacgg gcaagggcag
     1501 gggggaggtt cagctcctgc tccagccgta ttgctgctgc acttttttca ggcactttgg
     1561 cactttcatc aaacacccac cttttttcca tatatttagg aatggctttt gcataaaagt
     1621 agcaagcctg acgctcaatg agaaatcttt ttttggtctt gtttacggtt ggatgctccc
     1681 ttacagcact cgctcaggag gatctttcct tttattttgc agatgcacaa cccaatggag
     1741 gccgtaacct gacttcattt ccggaggcgt tcagaggaga atatatctcc aaaaaggaca
     1801 gtctgcttaa agtcaccatc actgaagact ccattcatgt gcgactgatc agctttgtga
     1861 tcatgacgct caaagaagtg aaagatgccc ctggattacg attaaaagac tcgcttttat
     1921 ttggtattta caaggataga cctgtggaag tgattcgaaa gaatgacacc ctgatatttg
     1981 gaatggaaac gaaatacagg atgttcgctg tgtcggacac ctctgttata cgtgtttgcg
     2041 gaaacgattt gttgttgagc agtaaggatg gaaatggtaa atggactgta atgcggatca
     2101 gtctgattcc tcgcgatgga ttaaaagctc cgggggttga atttgcctat gtcgatcacg
     2161 atgaacagat caaactgatc cgaagtcaga agggattgca acgcaaacag gaaaacggtc
     2221 agacttttct tcagttggat ttggcttacc cggacatgca acgttttatt gaggcaggtg
     2281 ggtttaatat gcgacaatca tatttccgca ccgaatacca ttgaggaaga tacgcgacat
     2341 actttttcac cgctattcat tactcgctat tgcagtagtg ctggtttttt caggcggtta
     2401 tttatacaga agttccttca tttctgttga tggaaaccgt ttagccgcta acgcagaaaa
     2461 tgttttgcgc gaatcggctg tctacgcaaa tgagtggttt aagtccatcc gcgttcaatt
     2521 gctttcaggg caaaaaaatg cagaaatcaa ttcgtcagag aatgagcttc atgccgttat
     2581 ggtatttcgt gagcgacaac cggttttctg gaccaattca cgtataccat tcgatacaac
     2641 tgcactggat acgcttggaa aatcaggatt gattcatttg cgcaacgggt ggtacttcta
     2701 tcaccatgaa caactggatg ccgaacattc gggtattgtt tttacgcagg taagaaagga
     2761 atatgtacac aagaacaggt acctgagcga tcattttcac agcgcatggg gaattccgga
     2821 tgaagttaat ctcgttgaac cgggcacaga aaattcgttt ttggtaagag atgaaaacgg
     2881 caatgcagta ttcgcgcttt tactttcgga agcaactatt acatcgggca gttcgcttta
     2941 tcttattgcc ggcttattgc ttttaggaat agtattgctc atccggtttc ttaaggaaga
     3001 atgtgaaaac ctggaaaagt ttatcggtcc tttctattcc agtctgcttt tggttttttc
     3061 ggttgttctt cttcgctact ggtcgctcag catggagttc cccgctgcat tttatgcatt
     3121 cgaattgttt caacccgatt tgtttgccgc gtcatttttt cttccctctc ttggcgatta
     3181 tctgattaac gcatttctga ttctctacct tttctatttt ctcgatgcac ggtcatcccg
     3241 ctttaattat catctctcct ctaacaaagg tgcagctatc ggtattattg cctttctttt
     3301 aggggtattg atggcatttg gtcattccat tgggttttta atgcagggat tgatcgaaga
     3361 ttcaaatatt gaattcaaca tcaataatat ttttaatctc agcacttata gtttcattgg
     3421 ttttaccatt gttggtttac tttattttgc cttttatctt ttttgtgatt ttacattcag
     3481 ggttgcaaaa aaactgcaga tcgaaaaact cacatttctg gtattgcttg gtgttttttc
     3541 catcttctcc acctttgcgc tctacctttg gggtgttaac gatccttttc agatcatttg
     3601 gcccctgacc attttgtttt tgttgtttgc agttcacttt gtccaggaga aagctcaatt
     3661 gagttttaat gttaccgtac tgctgctgat cattttttcc gggttcacgg cacatgccct
     3721 ttcaaaattt accggtgcga aagaacgtaa gaacagaatt gttttggcgg aaagactttc
     3781 taccgatgaa gacccattgg tggaaatatt gttttcagag atggaagccg gattacgcag
     3841 aagtgaattg ttgaatagtg cctttgattc ttctgcggtg ttttcgaaaa ctgccttcga
     3901 agaacaattg gaaaaggaat atttctcggg cgattggaat gcattcgaca tccagtttta
     3961 tttgtttgca gcagacagca cgcctgttgg tctcgaagga tttgctccga taagggaatt
     4021 ttcggatctc gataatgtta ttcttgagaa tggaattccg agtaagttca gtggtaatat
     4081 gaactttatt tacaattcca ccaataagct gagctatatc atcaagcttc cggtatctgc
     4141 acgtaatgag gccccaaaag gatatttgt
//
LOCUS       DAOMYG010000293         4136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_7648_length_4136_cov_8.178878, whole genome shotgun sequence.
ACCESSION   DAOMYG010000293 DAOMYG010000000
VERSION     DAOMYG010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7648_length_4136_cov_8.178878"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="PK610_09685"
     CDS             <1..1004
                     /locus_tag="PK610_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HRJ36247.1"
                     /translation="RKAFVLYRFPGEEEVFQLVEAQSYRPIRYDEIATENGFVVFPFS
                     GEQGLFLSELKGEKIPVQREIPKPRSICSKEEYLKIAESFVSSIKDGQFIKLVLSRRE
                     RIPIPSVFDPFFFFIQLCENYPEAFVHIGHYPGFGTWAGASPEGLLRTEKTYYLTMSL
                     AGTQLHEGRDPKTIRWEKKEKEEQFIVTKAIRKTLEENQITDYKYGAPYTHLAGHLAH
                     IRADFRFKRETVESTGKLIQSLHPTPAVGGMPQKEAQAYIDQHEGHDRSLYSGFLGEC
                     KTDGEVQLFVNLRCMQIMENELDLFIGGGITADSEPEEEWKETEQKATILKRFLGDL"
     gene            1155..2327
                     /locus_tag="PK610_09690"
     CDS             1155..2327
                     /locus_tag="PK610_09690"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HRJ36248.1"
                     /translation="MPVYQRHGKIPHKRHIVFRKPDGSLYHEELFGTEGFSGVSSLVY
                     HLNPPTIVKEAGEPWSVRPKIAVEDNLRARSYQGFNIPPTDDYLASRKTLFVNDDMTI
                     GLAAPRKSMNDYYFKNADADEMIFIHKGNGVLRSMYGSVDFEYGDYVIVPRGTVYQIK
                     FETEDNRLLIVESHSPITTPKRYSNDKGQFLEHSPFCERDFKLPYALETHEEEGEFKV
                     LIKKRGLIYPYIYGTHPFDVVGWDGYNFPYAFSIFNFEPITGRIHMPPPIHQQFEGRN
                     FVICSFVPRLYDYHPDAIPAPYHHSNIDSDELLYYVDGDFMSRNNIQKGQITLHPGGI
                     PHGPHPGAIERSIGKKETGELAVMIDPFNPVKITEEALAIEVKDYHKSWILQESAH"
     gene            2385..3542
                     /gene="hppD"
                     /locus_tag="PK610_09695"
     CDS             2385..3542
                     /gene="hppD"
                     /locus_tag="PK610_09695"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HRJ36249.1"
                     /translation="METKDLTKVQNYSYSGEKIFEKAKDFLPINGTDFVELYVGNAKQ
                     AAHYYKTAFGFQSLAYAGLETGVRDRTSYVIQQGKIKLILTTPFDPESEISHHIRKHG
                     DGVKVIALWVDDATSAFNETVKRGAKPFMEPHKEEDKDGFVIKSGIHTYGDTVHVFVE
                     RKNYNGVFLPGFQKWESEYNPADLGFKYIDHMVGNVELGEMNKWSKFYEEVMGFANLI
                     TFDDKDISTEYTALMSKVMTNGNGRIKFPINEPAEGKKKSQIEEYLDFYKGAGCQHIA
                     IATNDIVHSVSEMRKRGVEFLYVPGTYYDNVKDRVGIIEEDLQKLKSLGIMVDRDEDG
                     YLLQIFTKPVEDRPTLFFEIIQRKGANSFGKGNFQALFESIEAEQARRGTL"
     gene            complement(3572..>4136)
                     /locus_tag="PK610_09700"
     CDS             complement(3572..>4136)
                     /locus_tag="PK610_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36250.1"
                     /translation="KGTYMNARKEVRLLINRLLSSLTFSDYSELEVIAADFENLKAKY
                     ESLVRPDELLEIKYLLIAAYINTAEFKKAQKEIQLFFQIPKPEVRRDIQNLVRVLNVY
                     VQFRLGNFEYLEYLVRNTYLYFRKLNDPGEPETIILDHFRKMKEEYSEAGIDNFIESI
                     SSRLKSVDRDELNHLISFVFIKKTAEK"
BASE COUNT         1271 a          877 c          890 g         1098 t
ORIGIN      
        1 aaagaaaagc attcgtattg tatcgctttc ccggtgaaga agaagttttt caattggtgg
       61 aggcacaaag ctatcgtccg attcgttacg atgaaattgc aaccgaaaac ggatttgtag
      121 tttttccatt ttcgggtgaa caaggattgt ttctttctga actgaaaggt gaaaagattc
      181 cagttcaaag ggaaattcct aaacccagaa gtatctgttc aaaagaagaa tacctgaaaa
      241 ttgctgaatc atttgtttct tcaattaagg atggtcagtt tattaaactg gtactttcca
      301 gaagagaacg aattcccatt ccctctgttt ttgatccttt tttcttcttt attcaactct
      361 gcgaaaatta tcccgaggcc tttgtacata tcggacatta cccgggcttc ggcacatggg
      421 caggagctag tccggaaggg ttattaagaa ctgaaaaaac gtactacctg acaatgtcgc
      481 tcgcaggtac gcaactgcat gaaggtcggg atccgaaaac gattcgttgg gagaaaaaag
      541 aaaaggaaga acaatttata gttaccaaag ccatccggaa aacgctcgaa gaaaatcaaa
      601 ttacagatta caagtacgga gcaccttaca cccatctagc gggacatctt gcacatatcc
      661 gggccgactt cagatttaag cgcgaaactg tagaatcaac aggaaagctc attcagtcct
      721 tacatcccac acctgcagtt ggtggaatgc cacagaagga agcgcaagcc tatatcgatc
      781 agcacgaggg tcatgaccga tccttataca gtggtttttt gggtgaatgc aaaacagatg
      841 gcgaagtgca gctgtttgtg aatttgcgtt gcatgcaaat tatggaaaac gaacttgatc
      901 tctttattgg aggggggatc acggcagatt cagagccgga agaggagtgg aaagagacag
      961 aacagaaagc aactattctg aaacgctttc ttggtgattt gtaaaatcag gaaaacctcc
     1021 cggtttcaaa acagtttcct cctatttcaa aaaaatccaa aatcctgatt gagatcagga
     1081 ttcagaatta ccgcatctga cttcggtcat gttttttgca tttccacttt ccgagcttgc
     1141 ataaaactta ttttatgccg gtatatcaac gtcacggaaa gatccctcac aagcgtcata
     1201 ttgtatttcg caaacccgat ggatcattgt atcatgaaga actattcgga acagaaggtt
     1261 tttcaggggt gtcctcactg gtttatcacc ttaatccgcc aaccattgta aaagaggccg
     1321 gggagccatg gtcggtacga ccgaaaatag cggtagaaga taatttacga gcacggtcgt
     1381 atcagggctt taatatacct cccacggatg attatctggc gtcgcgcaaa acactttttg
     1441 taaatgacga catgaccatt ggtcttgctg caccgcgcaa atcgatgaac gactactact
     1501 tcaaaaatgc cgatgccgat gaaatgatct tcattcacaa aggcaatggt gttttacgca
     1561 gcatgtacgg aagtgtggat ttcgaatatg gagattacgt gatcgtcccc agaggaacgg
     1621 tatatcagat caaatttgaa acagaggaca accgtttgct gattgtagag agtcattcac
     1681 cgattacaac accaaaacgg tattcgaacg acaaaggtca attccttgag cattctcctt
     1741 tttgcgagcg cgatttcaaa ctaccctatg ctttggaaac gcatgaagaa gaaggcgaat
     1801 ttaaagtact gatcaaaaaa cgcggattga tctatcctta catctacgga acgcatccat
     1861 tcgatgttgt aggatgggac ggatacaatt ttccctatgc cttttctatt ttcaatttcg
     1921 aaccgattac cggtcgtatt cacatgccac cacccataca ccagcaattt gagggaagga
     1981 attttgtgat ctgttcattc gttccccggt tgtatgatta ccatcccgat gccattcctg
     2041 caccttacca tcacagtaat atcgacagcg acgagttgct ctattatgtg gatggagatt
     2101 tcatgagcag gaacaatatt cagaaagggc aaattacgct gcatcccgga ggaattccgc
     2161 acggaccaca tcccggtgcc attgagcgtt cgattggcaa aaaagaaacc ggagaattag
     2221 cggtgatgat cgatccgttt aatcccgtaa aaattaccga agaagcattg gccattgagg
     2281 taaaagacta ccacaaaagc tggatacttc aggaatctgc acactaagaa ccttccactc
     2341 catatatcaa tcaacaacaa gtttaacctc catcaataaa agatatggaa acgaaagacc
     2401 tcaccaaagt tcagaactac tcctattcgg gagagaaaat tttcgaaaaa gcaaaagact
     2461 tccttcctat taacggaaca gattttgtgg aattgtatgt tggaaatgca aagcaggctg
     2521 cgcattatta caaaacagct tttggatttc aatcgctggc ctatgccgga ctcgaaaccg
     2581 gagtacgcga tcgcacatcg tatgtgatcc aacagggaaa aatcaaactc atcttgacca
     2641 cgccctttga tccggaatcc gaaatttcac atcacatccg caagcacgga gacggtgtaa
     2701 aggttattgc actatgggtg gacgatgcca cttcggcctt taacgaaacc gttaagcgcg
     2761 gagctaaacc ttttatggag ccgcacaagg aagaagacaa agacggattt gtcatcaaat
     2821 cgggaattca cacctatggc gacactgtac acgtttttgt tgaacgcaaa aactacaatg
     2881 gcgtatttct tcccggattt cagaaatggg aaagcgagta caatcctgca gatttaggat
     2941 tcaagtacat cgaccacatg gtgggcaatg ttgaacttgg ggaaatgaat aagtggtcga
     3001 agttttatga ggaagtcatg ggctttgcca atcttatcac cttcgacgat aaagatattt
     3061 caacggaata cactgcgttg atgagtaagg tgatgaccaa cggaaatggt cgcatcaaat
     3121 tcccgatcaa cgaacctgcc gaaggaaaaa agaaatcaca gatcgaagaa tacctcgatt
     3181 tctacaaagg agcaggctgt cagcacatcg ctattgccac caatgatatt gttcattcgg
     3241 taagcgaaat gcgcaaacga ggtgttgaat ttctgtatgt tcccggaacc tattacgata
     3301 acgtaaaaga ccgcgttgga attattgaag aagatttgca aaagctaaaa tcacttggca
     3361 taatggtgga ccgcgatgaa gacggttatc tcctgcagat cttcaccaaa ccggtagaag
     3421 atcgtccaac cttgttcttc gaaatcattc aacgcaaagg cgccaactcg tttggaaaag
     3481 gaaatttcca ggccctgttt gagtcgattg aagcggagca ggcgcgtaga ggaacattgt
     3541 aattttccct ctcaaacggc agagaaatac actatttctc tgccgttttc tttatgaata
     3601 caaaagaaat caggtgattc aattcatctc gatctactga ttttaaacgc gaggaaatcg
     3661 attctataaa attgtcaatt cccgcttcgg aatactcctc cttcatcttt cggaaatggt
     3721 cgagaataat cgtctcgggt tcgccaggat cattgagttt tcggaagtag aggtaagtat
     3781 tgcgcacaag gtattcgagg tattcaaaat tacccagtcg aaactgtacg tacacattta
     3841 atacgcgcac caggttttga atatctcttc tcacctccgg tttaggtatt tgaaaaaaca
     3901 attggatttc cttttgcgct tttttgaatt cagcggtatt gatgtaagct gcaatgagca
     3961 aatatttgat ctcgagtaat tcatccggcc gtaccagaga ttcatatttt gctttcaggt
     4021 tttcaaaatc tgctgcaatc acttcaagtt ccgaataatc agaaaaggta agtgacgata
     4081 gcagtcgatt gatcagcaat ctcacctctt ttcgggcatt catgtaggta cctttt
//
LOCUS       DAOMYG010000294         4123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_7673_length_4123_cov_7.655113, whole genome shotgun sequence.
ACCESSION   DAOMYG010000294 DAOMYG010000000
VERSION     DAOMYG010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7673_length_4123_cov_7.655113"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PK610_09705"
     CDS             <1..680
                     /locus_tag="PK610_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36251.1"
                     /translation="IGAELRPNPNWMINVYFDSFESNWLRSGVTGPMRGYEFLGQLQW
                     RPNKKLDMYFRVRTRTKPGNDPNDPEAIDVLLDKEQNNFRYQFSYKASDAITLRSRFE
                     WMDVGYSNQEKEKGYVIYQDVVWKPMSSPLSLTARYALFDTDSYNSRIYAYENDVLYV
                     FSIPAYYYRGSRFYGILRYQYKKRFDIWLRYAQFLYNNRTSVGSGMNEIEGNRKSEVR
                     IQIRFSF"
     gene            complement(670..1062)
                     /locus_tag="PK610_09710"
     CDS             complement(670..1062)
                     /locus_tag="PK610_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36252.1"
                     /translation="MKRAYLLSGTFLYLLLYFNKHIFQFDFPFRHQLSNVLAVPLITG
                     WIAWSMQKFWSTRFVPERGMVWMTFVVISIYFEFILPQWSASATGDPFDLLAYFFGTI
                     LWFFLIEKKEHELNDKAQVLIKSALIRN"
     gene            complement(1059..1361)
                     /locus_tag="PK610_09715"
     CDS             complement(1059..1361)
                     /locus_tag="PK610_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36253.1"
                     /translation="MKAEIKCPACLAWVSSSDGHCTHCGSVLDRNVRLKQDRISRGVS
                     VGPPERKKTRIDLWLERTEHSENTLVVVLRAIVGVAWAIYMAVLGFIVWLVTLLAG"
     gene            1474..2115
                     /locus_tag="PK610_09720"
     CDS             1474..2115
                     /locus_tag="PK610_09720"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRJ36254.1"
                     /translation="MKTLRILSFSVLAAALFTACSSDAVESTDAKDVANAAAESIVYK
                     VDVKASTLGWYGSKLAYGHQGVIDIISGELTMKGDTLTSGNFTVDMKTVKATDEGGDP
                     EKMAKLAGHLMAADFFDAEKYPTSKFEITGATKSQDGTYNVSGNMTIKDVTKNISFPA
                     TIKVEGDKLTATAEFTINRNDWGVTYGSGVSGAIGDAVISDDIKYNVSLVASK"
     gene            2285..>4123
                     /locus_tag="PK610_09725"
     CDS             2285..>4123
                     /locus_tag="PK610_09725"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRJ36255.1"
                     /translation="MRLFLFVSLSILTCITASAQLQPVTFKNDPIGLKQYQLKNGLTV
                     FLSENHDIPQVFGAVVVKTGGKKDPADNTGMAHYLEHMLFKGTEELGTTNYVQEKIHL
                     DEINRLYDELGKTRDPLQRKIIQKKINDESVKAAQYAIPNEMDRMLSEIGSDDVNAFT
                     TEEITAYFNTFPPNQIRRWLEIYDHRFEKPVFRLFQSELETVYEEKNRGMDNPIEFVL
                     EKYMQKFYKVHPYGQQTVIGKTEHLKNPSLTAMYNYFNTYYVANNMALILSGDFKSEE
                     VIRIIEEKFSDWRSAPIPEFPNYREEDFKGRELVKMRATPVKAGAIGFRVPAKNANDE
                     TEVEVLLNMLSNESAGPLDRKKREGKLLECGAFPLINDDYGAAIFFFIPKLVGQSLKK
                     AERVIRAEISNIKKGEFDERLLEATKISLIKYFQQNWERNESRVLSIGTAFSSGTSWS
                     EFLSYESKIRAVNKDDIVRVANKYLGENSLVLHSKMGRPDKEKLEKPGFEPVIPKEEL
                     QSDYYKKWKTIPEDEAITRFVDFEKDIKEYKLGDKTTLYQSTNPYNSVFSIKLRFGTG
                     KFYEPDLKIAPDYLQYAASDYFTAIEFNKALYNIGSEVDFYVTEEEF"
BASE COUNT         1350 a          889 c          837 g         1047 t
ORIGIN      
        1 acatcggagc agaattgcgt cccaatccaa actggatgat caatgtttat tttgattcat
       61 tcgagagcaa ttggcttcgt tcaggagtta ctggtccgat gcgtggttac gaatttcttg
      121 gtcagctaca atggcgtccc aataaaaaac tcgatatgta tttccgcgtg cgcacccgca
      181 caaaacccgg caacgacccc aatgatccgg aagcgattga tgtactgctg gataaggaac
      241 aaaacaattt ccgatatcag tttagctata aagcaagtga tgcaattaca ttaagaagcc
      301 gtttcgagtg gatggatgtt ggttacagta atcaggaaaa agaaaaagga tatgtcattt
      361 accaggatgt agtatggaaa ccgatgtcat ctcccctttc cctcaccgcc cgttacgcac
      421 tattcgatac cgactcttac aattccagaa tttacgctta cgaaaatgat gtattgtatg
      481 tcttttccat tcctgcctat tattacagag gcagtcgttt ttacggcata cttcgttatc
      541 agtacaaaaa gcgattcgat atctggctgc gttacgcaca attcctttac aacaacagaa
      601 caagtgttgg atcaggcatg aatgaaattg agggtaatcg aaaatcagaa gtaagaattc
      661 agatccgttt tagtttctga ttaatgcgga cttaattaaa acctgggcct tatcgttcaa
      721 ttcatgctct ttcttttcaa tcagaaaaaa ccagagaatt gtcccgaaaa aataagcaag
      781 caaatcaaag ggatctcccg tagccgaagc agaccattgc ggaagaatga attcaaaata
      841 aatactgatt acaacaaaag tcatccaaac cattcctcgt tccggaacga aacgggtcga
      901 ccagaatttc tgcatgctcc atgcaatcca ccctgtaata agaggtaccg ccaatacatt
      961 tgaaagctgg tgacgaaatg gaaaatcaaa ctgaaaaata tgcttgttga agtaaagcag
     1021 aagatagaga aatgttccgg aaagcaaata agcacgtttc atcctgccaa tagtgttacc
     1081 aaccacacaa taaacccaag cactgccata taaattgccc aggcaacacc aacaatagcc
     1141 cttaaaacaa ctaccaatgt attttccgag tgctccgttc gctccagcca aagatcgatc
     1201 cgggtctttt ttcgttccgg cggaccaaca gatacccccc tgctgatcct gtcttgttta
     1261 agtcggacat tccggtcgag cactgaacca caatgcgtac agtgcccgtc ggaagaggaa
     1321 acccaagcca aacaagcggg acatttaatt tcagctttca tactataatg ttaaacaaaa
     1381 ttaagaaagt ggtgttttaa taaagaattt cttgctcaat tcaattgttg tatatacatt
     1441 tgttgaacta acttaaaaca aaacatttaa cccatgaaaa cacttagaat tctttctttt
     1501 tccgtgcttg cggcagcact ttttacagct tgtagcagcg atgcagtaga atcaactgat
     1561 gctaaagatg ttgccaatgc agcagcagaa agcatcgttt acaaagtaga tgtaaaagcc
     1621 agtacacttg gttggtacgg ctctaaatta gcttatggtc accagggtgt tatcgacatc
     1681 atctcaggcg aacttactat gaagggcgac acacttacca gtggtaattt tactgttgat
     1741 atgaaaacag taaaagcaac tgacgaaggt ggagatccgg aaaaaatggc gaaacttgcc
     1801 ggtcatttaa tggcagcgga ttttttcgat gctgaaaaat atcctacttc aaaatttgaa
     1861 attacgggtg caaccaaaag tcaggatggc acttacaatg ttagcggaaa catgaccatt
     1921 aaggacgtaa ccaaaaacat cagcttccct gctacaatta aagtagaagg tgacaagctt
     1981 actgcaactg cagagttcac catcaaccgt aacgattggg gtgtaactta tggttcaggt
     2041 gttagcggtg caattggtga tgccgttatc agcgacgata ttaagtacaa cgtgagcctt
     2101 gtagcctcta aataagtttt cagggtttta gggctacctg gaggcgccgg tcatacgacc
     2161 ggcgttctct tttttagggg ggtagcaaag aaaatctgcc tgccaacgcc aattgcaact
     2221 gcatcgtact gcatgaaata gtccgccaaa aatcctcata tttaccatta taaaaacagt
     2281 actgatgagg ctatttctat ttgtttcact gagcattctt acctgtatta cggcaagtgc
     2341 ccaattgcag cctgtaacat ttaaaaatga cccaattggg ttgaaacaat accaattgaa
     2401 aaacggacta accgtattcc tgtcagagaa ccatgatatt ccacaggtct ttggtgcggt
     2461 ggttgttaaa acaggaggga aaaaagatcc tgccgataac accggtatgg ctcattacct
     2521 cgaacacatg ctattcaagg gcactgaaga actgggtaca accaattatg tacaggaaaa
     2581 aattcacctt gatgaaatta atcgcctgta cgatgaactg ggaaaaacaa gagatccttt
     2641 acaacgcaaa atcatccaga aaaagatcaa tgacgaatct gtaaaagcag cacaatacgc
     2701 tattccaaat gagatggacc gcatgctttc tgaaattggt tcagatgatg taaacgcctt
     2761 caccaccgaa gaaatcaccg cctatttcaa taccttccct cccaaccaga ttcgcagatg
     2821 gcttgagatt tacgatcatc gttttgaaaa accggtgttc cgcctttttc agagtgaatt
     2881 ggaaaccgtt tacgaagaga aaaacagagg aatggataat cccattgaat ttgtgctgga
     2941 aaaatacatg cagaaatttt ataaagtcca cccgtacgga caacaaactg taatcggaaa
     3001 aacggaacac ctcaaaaatc catcgcttac tgcgatgtac aactatttca acacgtatta
     3061 cgttgccaac aatatggcgc tgattctatc cggagatttt aaatcggaag aagtgattcg
     3121 catcatcgaa gaaaaatttt ccgactggcg ttccgctcct atccctgaat ttcccaacta
     3181 cagggaagaa gattttaaag gacgcgagct ggtaaaaatg cgcgcaactc cggtaaaagc
     3241 aggagccatt ggattcagag tgcccgctaa aaatgcgaat gacgaaactg aagttgaagt
     3301 ccttctcaac atgctctcca atgaaagcgc aggcccactc gatcgcaaaa aacgcgaagg
     3361 caaactgctg gagtgcggcg cattcccatt gatcaatgac gactatggag cggcaatctt
     3421 tttctttata ccaaaactgg ttggtcaatc cctgaaaaaa gcagaacgcg taatacgtgc
     3481 tgagatcagc aacattaaaa aaggagaatt tgatgaacgt cttctggaag ccaccaagat
     3541 ttcgttgatc aaatactttc agcaaaattg ggaacgcaat gaaagtcgcg tactgtccat
     3601 cggaactgct ttttcatccg gcacttcctg gagtgaattt ctgagttacg aatcgaaaat
     3661 acgcgcagta aacaaagacg acattgtgcg ggtggccaac aaataccttg gagaaaattc
     3721 gctggtactt cattccaaaa tgggcaggcc cgataaggaa aaactggaaa agcccggttt
     3781 cgaacctgtc attccaaaag aagaactgca atcggattat tacaaaaaat ggaaaacaat
     3841 tcctgaagac gaagcgatca cccgttttgt ggatttcgaa aaggacatca aagaatacaa
     3901 gttgggcgat aaaaccacgt tgtaccaatc aacaaatcca tacaattccg tgttcagcat
     3961 caaactgcgt tttggtactg gaaaattcta cgaacccgat ctgaaaattg ctcccgatta
     4021 cctgcaatat gctgcatccg attatttcac cgcaatcgaa ttcaataaag cgctttacaa
     4081 cataggatca gaagttgatt tttatgtgac agaagaagag ttc
//
LOCUS       DAOMYG010000295         4053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_7867_length_4053_cov_11.348424, whole genome shotgun sequence.
ACCESSION   DAOMYG010000295 DAOMYG010000000
VERSION     DAOMYG010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7867_length_4053_cov_11.348424"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3878
                     /locus_tag="PK610_09730"
     CDS             <1..3878
                     /locus_tag="PK610_09730"
                     /inference="COORDINATES: protein motif:HMM:NF038117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor I family protein"
                     /protein_id="HRJ36256.1"
                     /translation="VELVATRTINFNQSPYTVVFNNNGTANANGWAAGGGLSYAFQIS
                     SGTVNAGDVVYVGGSTMAIAGTKLRIIDCTTQGGDGFGNAGGTGGVLGNGGANADGIA
                     VFNVPSAQITSTTIPVDAIFFGTGLGTAVVAGGTAGYQLPLSDIYPGGKLQSTSFIAA
                     DPGSGVTIVATGVFNRTTGTFSTGRGWATGTTTDGTTSITLQQSLTPATVVFASNNQT
                     INENAGTVNINLTVSNSNAEPIFVELYVKPISNASTSDYSVSSYWLSIPAATNGTHSF
                     PITITNDNDLESDEYLLFTFSGLVNATAGANNHHTFYIKDNDNPAPVANNELLLSLLN
                     SFSNGGTGNSAEIVVHDPGTQRLFIANSIGRKLDIVNFANPSAPVLLTSIDLTPYGNI
                     NSVDVYNGIVACSIEDGANPQDPGKVVFFDASGNFLNSLVVGAMPDMVKFTHNGTKVL
                     TANEGEPTSVDLHPSALPSYTQDPNGSISIIDVSGGIASLSQANVTTIDFTAYNGQEA
                     MLRSQGIRLFGVFGGASSASQDLEPEYISISEDDQVAWITMQENNAIAKINLSNNTLV
                     SLEPLGFKDHSLTGNAMDVSDQTAGINLSNFPVKGMYMPDAIANFTVGGITYIVTANE
                     GDAREYTGAIIESVRLSTLNLDATAFPNGADLKRAQLAGRLNVTNQLGDIDNDGDFDE
                     IYAFGARSFTIWNGTTGALIYDSGDQLERTIAQHPVYSTMFNVSNANNTLKNRSDDKG
                     PEPEGVATATINGIPYLFVSLERVGGVMAYNVTNPASPQYVGYWNNRNLAGLGPDLGA
                     EGIIYINAAQSPNGNSILILANEVSSTLSIFQVQTCQQTLPLTVNSSGNTICANDDVT
                     LSVSNPTGANFQWIKDGNPISGETSSSITVNAAGAYQVQATSVAFGCSASSTAQTVVV
                     NALPTVGINASSLSVCAGSALTLSGTGAQSYSWDNSVADGVAFSPSSTLTYTVVGTDI
                     NNCQNSSVITVPVNSLPNVGGSVAPNPVCIGSQATFTGNGASSYVWDNGVSNGVPFVV
                     NADQSFTVTGTDANGCTNSTTVTVSTLPLPVVNGNVTDNSICAGESVTFSGSGAVSYA
                     WSNGVTNGLPFSPSSTQTYTVSGTGSNGCVGTAQVTVTVNPIPAIPVITVNMDTLFSA
                     TADNYQWYFNGSSLAGQTNQYLVVSTNGNYYVVVTSDEGCSNQSTVYNYNAVGVQEAN
                     GMNKMEIFPNPFNHQTTLRIHSGVHGHVGIRITDAIGRTISNRQFEIHAGMNDILLSG
                     EQFESGSGVYFISIETGTQTKTLRLVKVI"
BASE COUNT         1156 a          796 c          876 g         1225 t
ORIGIN      
        1 gggtggagct tgtggctaca cggacaatta attttaatca atctccatac actgttgtat
       61 tcaataataa tggaacagct aacgcaaatg gatgggctgc aggtggtggc ttatcgtatg
      121 catttcagat aagctccggc acagtaaatg ccggggatgt tgtatatgtt ggtggttcaa
      181 caatggccat tgcaggtaca aaattgcgca tcattgattg tacaacacaa ggtggggatg
      241 gatttggaaa tgccggcgga acaggaggtg tattaggtaa tggtggtgca aatgcagatg
      301 gtattgctgt atttaatgtg ccctctgctc aaataacctc tactaccata cctgttgatg
      361 ccattttctt tggtactggt ttgggaacag ctgttgttgc agggggaact gcaggatatc
      421 agcttccgct tagtgatatt tatcccggtg gaaaattaca aagcacaagc tttattgcgg
      481 cagatcccgg aagtggagtt acaatcgttg caaccggagt tttcaatcgt acaacaggta
      541 cttttagtac aggtcgaggt tgggcaaccg gtactacaac tgatggaaca acatcaatta
      601 cccttcaaca atctttaact cctgcaactg tagtttttgc ttcaaacaac cagaccataa
      661 atgaaaatgc aggtacggtc aatattaatc tgacggtttc aaattccaac gctgaaccca
      721 tttttgtaga gttgtacgta aaaccaattt caaatgcgtc aacatccgat tattcagtga
      781 gcagttattg gttgtcgatt ccggctgcta caaatggtac gcattctttt ccaataacca
      841 taacaaatga caacgatttg gaatcggatg aatatttact gtttacattt tccggtttgg
      901 taaacgcaac tgcaggtgca aataatcacc acacatttta cataaaggat aatgataatc
      961 ctgcaccggt tgcaaataat gaactgttgc tttctttgtt gaattcattt agcaacggag
     1021 gcacaggaaa ttcggctgaa attgtagttc atgatccggg aactcagcgc ctttttattg
     1081 caaattcaat cggacgaaag ttggatattg tcaattttgc aaatccttct gcaccggtat
     1141 tgcttacctc tattgattta actccttacg gaaatataaa ttctgttgat gtttataatg
     1201 gaatagttgc ctgttcaatt gaagatggtg ccaatcctca agaccccggt aaagttgtgt
     1261 tttttgatgc ttccggaaat tttctgaata gtctcgtcgt tggagcaatg cccgatatgg
     1321 tgaagtttac gcataacggt acaaaggttc tcactgcaaa cgaaggtgaa ccgacaagtg
     1381 tggatttaca cccttctgca ttaccatcgt atactcaaga tccaaatgga tcaataagca
     1441 tcattgatgt aagcggtgga atagcgtctc tatcacaagc aaatgttaca acgattgact
     1501 tcactgctta taatggacag gaagcaatgc ttcgttcaca gggaattcgt ttgtttggtg
     1561 tgtttggagg agcctcttca gcatcacagg acctcgagcc tgaatatatt tcaatatcgg
     1621 aggatgatca ggttgcatgg attacaatgc aggaaaataa tgcaattgca aaaatcaatc
     1681 tctctaacaa cacgcttgtc tctcttgagc cgttagggtt taaggatcat tccttaaccg
     1741 gaaacgcaat ggatgtaagc gaccagactg caggaattaa tctttccaat tttcctgtta
     1801 aaggaatgta tatgcctgat gcaatcgcta attttacggt aggcggcatt acatatattg
     1861 ttactgcaaa cgaaggggat gcacgtgaat ataccggtgc aattattgaa tcagttcgct
     1921 tatctacttt aaatcttgat gcgactgcat ttccaaacgg agcagattta aaacgcgcac
     1981 aacttgcagg tcgtctaaat gtaactaatc agttgggcga tattgataac gatggagatt
     2041 tcgatgaaat ttatgcattc ggtgcacgat cgtttacaat ttggaatgga acaactggtg
     2101 ctttgattta tgacagtggt gatcaacttg aacgcacaat tgctcaacat ccggtttatt
     2161 caaccatgtt taatgtatcg aacgcaaaca acacattaaa gaaccgttca gatgataaag
     2221 gaccggaacc ggaaggcgtt gcaactgcaa ccatcaatgg aattccttat ttgtttgttt
     2281 cgctagaacg tgtaggtgga gtaatggcct ataatgtaac caatcccgca tctccacaat
     2341 atgttggtta ttggaacaac aggaatcttg ctggacttgg tcctgacctc ggagctgaag
     2401 gaattattta tatcaatgca gcgcaatctc ctaacggaaa ttccattctt attcttgcca
     2461 acgaagtatc ttcaacgctt tcgatcttcc aggtgcaaac ctgccaacaa acccttccgc
     2521 ttaccgttaa ttcgtccggc aataccattt gtgccaatga tgatgtaaca cttagcgttt
     2581 ctaatccaac cggagcaaat ttccagtgga ttaaagacgg aaatccaatt tcaggcgaaa
     2641 caagttcaag catcaccgta aatgcagcag gtgcctacca ggttcaggct acatctgttg
     2701 cctttggatg ttctgcaagt tctactgctc aaactgtagt ggtgaatgca cttccaactg
     2761 ttggcatcaa cgcatcatcg ctttcagttt gtgctgggtc tgcattaaca ttaagtggaa
     2821 cgggtgctca atcttattcc tgggataaca gtgtggcaga tggtgttgct ttttctcctt
     2881 cgtctacttt gacgtatacc gttgttggta ctgatattaa taactgtcag aattcatctg
     2941 ttattactgt tcctgtaaat tcacttccca atgttggagg atcggttgct ccaaatcctg
     3001 tttgtattgg atctcaagct accttcactg gtaacggtgc ttcaagttat gtttgggata
     3061 atggtgttag caatggtgtt ccattcgttg ttaacgccga tcagtcgttt acagtaacag
     3121 gaacagatgc caacggttgt acgaactcaa caactgtaac agtaagtact cttcctctgc
     3181 cggtagtaaa tggaaatgtt actgataatt cgatttgcgc gggagaatca gttactttct
     3241 ctggaagtgg tgcagtttct tatgcgtggt cgaatggagt aactaacgga ctaccgtttt
     3301 ctccatcatc aacacaaacg tacacagttt ccggtaccgg atcgaatggt tgcgttggaa
     3361 cagcacaagt aactgtaaca gttaatccga ttccggctat tccggtgatc accgtaaata
     3421 tggatacctt attctctgca actgctgata attaccaatg gtatttcaac ggtagttcac
     3481 tcgccggtca gaccaatcaa taccttgtgg tgtctaccaa tggaaattac tacgttgtgg
     3541 ttacctctga tgaaggatgc agtaatcagt caactgttta caattacaac gcagttggtg
     3601 ttcaggaagc aaatggaatg aataaaatgg agatcttccc aaatccattc aatcatcaaa
     3661 caacgcttcg tatacactcg ggtgtgcacg ggcatgtggg tattcgcatt acagatgcaa
     3721 tcggtcgtac aatttccaat cgtcagtttg aaatccatgc cggaatgaat gatatccttc
     3781 tttcgggtga acaatttgag tcgggtagtg gggtttattt tatttcaatt gaaacgggca
     3841 ctcaaacaaa aacgttacga ttggtaaaag tcatttgaga attatcttta atagttaaag
     3901 aaaagcggtc atgagagtga ccgctttttt tgtgaaaatc atgtgaagat gaaagattaa
     3961 ttgaaaggct tgttttttga ttttaatagt gctgaattat tgtgcaatgc cccaaatttg
     4021 aaatttaaat gagtcatagc tttccttaag gtt
//
LOCUS       DAOMYG010000296         4036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_7908_length_4036_cov_10.507410, whole genome shotgun sequence.
ACCESSION   DAOMYG010000296 DAOMYG010000000
VERSION     DAOMYG010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7908_length_4036_cov_10.507410"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PK610_09735"
     CDS             complement(<1..489)
                     /locus_tag="PK610_09735"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015362068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRJ36257.1"
                     /translation="MAKVALITGVTGQDGAYLSELLLSKGYIVHGIKRRSSLFNTDRV
                     DHLYKDQHESNVNFVLHYGDLTDSTNLIRIIQEVQPDEIYNLGAQSHVQVSFETPEYT
                     ANSDAMGTLRILEAIRILKLEKKTKFYQASTSELYGKVQEVPQKETTPFYPRSPYAVA
                     KLY"
     gene            complement(557..1357)
                     /gene="cysQ"
                     /locus_tag="PK610_09740"
     CDS             complement(557..1357)
                     /gene="cysQ"
                     /locus_tag="PK610_09740"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HRJ36258.1"
                     /translation="MKALVELAVRAALCGGKEILEVYEKDFSVEYKDDNSPLTEADQR
                     ANLIIEESLRIAGKPILSEEGRSIPYDERKGWNELWIVDPLDGTKEFIKKNGEFTVNI
                     ALIKNHFPVLGVIYVPVTGVLYFGANDIGAWKAVVPVKRGVSIPSMNEIEQSAERIPI
                     TRGESSPFRIVASRSHMSPETEVFIREQEKINGKAELVSMGSSLKICLVAEGSADLYP
                     RFAPTMEWDTAAGQAIAEAAGKKFIDFGTKERMRYNREDLLNGWFLVG"
     gene            complement(1361..3133)
                     /locus_tag="PK610_09745"
     CDS             complement(1361..3133)
                     /locus_tag="PK610_09745"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRJ36259.1"
                     /translation="MGWEGLVVISVIGFLIVSLYYDLIRPVIVFFLSAILFMTTGIIS
                     SHDFMTGFANEQIVVVVLLLMLSEIIQKTAVMDVVFRNVFSPGLSYRGFLAKMTTFVA
                     PISGFVNNTPLVAILMPYVYNWARKKEISPSKVLIPLSYATIIGGTITLVGTSTNLVV
                     NGFVTEAGFPSLCMFDFTIVGIPLFIVGVFYITFIASRLLPDRKDALKSFKDASREYV
                     VETLIGKDSQLIGKTVEDAGLRKLEGLFLVEILREEERISPVSPKQTLQCDDILIFAG
                     MTSTIIDLVKTNNGLTLPKHSQLPGQDQMQVVEVVLSSNSDLVGKKVKSTNFRGKYDA
                     AIVAVNRNGERITGKIGETELRTGDLLLLVAGKDFDNRAQDEQDFYVISRVKEIQNVT
                     SKQLIVLFGGAIAVFVASAIGWVTLFQGLLLLFCVIAAVKLVRFNEVKKSFDVDLYLI
                     LVFALSFGKAIANSGIDQTIGGYITQISGNSQNMVVILALVYLVTNILAMLVTNKAAV
                     AIMFPVTVAITKNLGVDPTPFFLAIAFAGSAEFVTPYGYQTNLMVYGPGGYKFKDYVK
                     VGWGLSVIYMIVCVSILSYWYKLV"
     gene            complement(3145..4026)
                     /locus_tag="PK610_09750"
     CDS             complement(3145..4026)
                     /locus_tag="PK610_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRJ36260.1"
                     /translation="MARLEVLKTYKIFIGGKFPRTESGRYYSPRDKKGNLLANVCLCS
                     RKDLRESVVAARSAFGGWSTRSAFNRSQILYRIAEMMEGRRTQFIDELIKQGATASAA
                     KKEVDLAVDRLVYYSGWCDKFQQVFSSVNPVNSSHFNFSVPEPTGVVGLLADEKTSLA
                     GLVSGIAALVAGGNTVIALASESKPLCAVTFSEVIATSDVPGGVINILTGSKKELLEH
                     FGSHMDINAVVYQGNSNEELSQLRSLASNNVKRISSYDKDWMDTAQQDPYFIMDAQEI
                     KTTWHPIENIGSEGSGY"
BASE COUNT         1264 a          952 c          696 g         1124 t
ORIGIN      
        1 atacagtttg gcaactgcat aaggactccg gggataaaaa ggtgtagttt ccttttgagg
       61 cacttcctga acttttccat acaattcgga agtagatgcc tggtaaaact tagttttttt
      121 ctcgagtttg agaatacgaa tagcttccaa aattcgcaaa gtccccatag catccgaatt
      181 ggccgtgtat tccggagttt caaacgaaac ctgcacatgc gactgagccc ctaggttata
      241 gatttcatca ggctgaactt cctgaataat gcgtatgagg tttgtactgt cggtaagatc
      301 accgtaatgc aatacgaagt tcacattaga ctcatgctga tccttgtaaa gatgatcaac
      361 acgatcggtg ttaaacaacg agctccttct cttgatacca tgaacaatat aacctttgct
      421 caaaagcaat tcggaaaggt aggcgccatc ttgacctgtg acccctgtaa tcaatgcaac
      481 ttttgccatt cttcagattt acagcaaaag taacaaaatc aacccgtatg gggatgaatg
      541 ccgttaataa agcgcctcag ccaacgagga accaaccatt caacagatct tccctgttgt
      601 aacgcatgcg ctcttttgtg ccgaaatcaa tgaatttctt tcctgctgcc tcagcaattg
      661 cctggccggc tgcagtatcc cattccatag ttggggcaaa gcggggatag agatcggcac
      721 ttccttcagc aacgagacag atttttagtg aactccccat cgaaacgagc tcggcctttc
      781 cgttaatttt ctcctgctcc ctgataaaca cctctgtttc aggactcata tgagaccggc
      841 ttgcgacaat tctgaaaggc gagctttctc cccgtgttat agggattcgt tcagcagact
      901 gttcaatctc gttcatggag gggattgaaa ctcctctttt gacaggaact actgccttcc
      961 atgcgccaat gtcatttgct ccaaagtata aaacacctgt aacaggaaca taaatcactc
     1021 ccaggacggg aaaatgattt tttataagcg caatattgac cgtaaactca ccattctttt
     1081 ttataaattc tttggttcca tcaagtgggt caacaatcca gagttcattc catcctttcc
     1141 tttcgtcata cggtattgat cggccttctt cacttaaaat aggtttacct gcaatacgaa
     1201 gggattcttc aattatcaaa ttcgctcgtt ggtcagcctc tgttaatggc gaattatcgt
     1261 ctttgtattc tactgaaaaa tctttttcat aaacctccag aatttccttt ccgccacata
     1321 atgcagccct aacagccagt tcaaccaatg ctttcataca ctaaaccaat ttataccagt
     1381 aactcaaaat tgaaacacag acaatcatgt aaataacgga taaaccccag cctactttta
     1441 cataatcctt aaacttataa ccacctggac cataaaccat caaattggtt tgataaccat
     1501 aaggagtaac aaactcagct gaacctgcaa aagcaattgc tagaaaaaat ggagttggat
     1561 caacacctaa attcttagtt atagctacag taaccggaaa cattattgct accgctgctt
     1621 tattcgttac cagcattgca agaatatttg tcaccagata gaccaatgca agaattacta
     1681 ccatattttg ggaattgccg gaaatctgag tgatatatcc gccaattgtt tgatcgattc
     1741 cgctgttggc aatcgcttta ccgaagctca aggcaaagac caatattaaa tacaagtcga
     1801 catcaaatga ttttttgacc tcattaaaac gaaccaattt aacagccgct ataacacaaa
     1861 acaaaagcaa taaaccctga aacaaggtta cccacccaat tgcagaagcg acgaaaacag
     1921 caattgctcc tccaaaaaga acgataagtt gtttcgacgt cacattctga atttccttta
     1981 ctctggaaat cacataaaaa tcctgttcat cctgagccct gttatcaaaa tcttttccgg
     2041 caaccaataa tagtaaatca ccggtacgta attctgtttc tccgatttta cctgttattc
     2101 gttcaccatt tctattcaca gctacaattg cagcatcgta tttgcctctg aaattggtcg
     2161 acttaacctt cttgccgacg agatcagaat ttgaagaaag tacgacttcc accacttgca
     2221 tttgatcttg ccccgggagc tgggaatgtt tggggagcgt aagaccattg tttgttttta
     2281 caagatcaat tatggtggag gtcattcccg caaaaatcaa aatatcgtca cattgaagag
     2341 tctgttttgg actaacaggc gaaattcgtt cctcttccct cagaatttca accaaaaaca
     2401 atccctccaa ttttctaagg ccggcatctt caaccgtctt ccctataagt tgtgaatctt
     2461 ttccaattaa agtctcaaca acatactctc tcgacgcatc cttaaagctt ttcaaagcat
     2521 cttttctatc cggaagaagc cgtgatgcaa taaaagtgat ataaaaaaca cctacaataa
     2581 acaaaggtat tcctactatc gtaaaatcaa acatgcacaa tgaggggaag cccgcttctg
     2641 taacaaatcc attaactaca agattagttg aagtacctac caatgtaatt gtcccaccta
     2701 ttatagtagc ataagacaat ggaataagca ctttagaagg tgagatttcc tttttacggg
     2761 cccaattgta gacataaggc ataaggatag caaccagagg cgtgttattt acaaaaccgg
     2821 aaattggtgc tacgaacgtg gtcatttttg ccaaaaatcc tctatagctt aaccctggtg
     2881 aaaatacatt tcgaaaaaca acatccatca cagctgtctt ttgaattatt tcagagagca
     2941 taagcaacaa aacaacaaca acaatttgtt cattggcaaa acccgtcata aaatcatgac
     3001 tggaaataat tccggttgtc ataaatagga ttgccgacaa aaagaaaaca ataacgggtc
     3061 gtatcagatc ataatacaac gaaacgatta aaaaaccgat tacactgata acaactaaac
     3121 cctcccagcc catattacca tgcgttaata tccactgcct tcagaaccaa tattctcaat
     3181 tggatgccat gtcgttttta tctcctgagc gtccattata aagtaaggat cttgctgagc
     3241 agtgtccatc cagtctttat catacgagga aatacgcttc acattattac tcgctaatga
     3301 acgaagctga gaaagttcct cattgctgtt tccctgataa actacagcat tgatatccat
     3361 atgagagcca aaatgctcaa gcagttcctt tttacttcct gttaaaatat ttattactcc
     3421 gcccggaacg tcagaagttg caatcacttc agaaaaagta actgcgcaca aaggtttcga
     3481 ctctgaagca agagcaatca cagtatttcc gccagcaact aatgcggcaa tcccggatac
     3541 aagtccggca agtgatgttt tttcatccgc taacaatcca acaacaccgg ttggttcagg
     3601 aacagaaaaa ttaaaatgag aactattcac aggatttacc gaactgaaga cctgttggaa
     3661 tttatcacac cagcccgaat aataaaccaa acgatctaca gccagatcaa cctccttttt
     3721 agctgcagat gctgtggctc cctgtttaat gagttcatct atgaattgag ttcgccggcc
     3781 ttccatcatt tctgcaattc ggtacaggat ctgagaccga ttgaatgcag atcgcgttga
     3841 ccagccccca aatgcagatc gcgccgcgac aacagattca cgcagatcct ttcgcgaaca
     3901 caggcaaacg tttgccagaa gatttccttt cttatcccgc ggagaataat atcttccact
     3961 ttccgtacgc ggaaacttcc ctcctataaa aatcttatac gtctttagaa cttccaatcg
     4021 agccattgtt tagaaa
//
LOCUS       DAOMYG010000297         4032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_7919_length_4032_cov_9.167463, whole genome shotgun sequence.
ACCESSION   DAOMYG010000297 DAOMYG010000000
VERSION     DAOMYG010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7919_length_4032_cov_9.167463"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /gene="rodA"
                     /locus_tag="PK610_09755"
     CDS             <1..1007
                     /gene="rodA"
                     /locus_tag="PK610_09755"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRJ36261.1"
                     /translation="QFGIQPWEFAKIGVSLALAKFLSTPQIRFEDTTTRLYTALFIGF
                     PAALILLQPDAGTVIVFISFIFVLYREGMSGNLLLFGLFVLVLSILSLMLKESTMELP
                     IIGTEVAGHIGLVIILTIIALLVLLLIRQFVMKRGRKTAILVLVLAYFSSIVIVFGTN
                     YAFYNVMKRHQQERIDIMLGLIDDPDGKAYNIDRSMAAIGSGGLSGKGYMKSTLANRG
                     QKHVPMQSTDFIFCTLSEEWGFAGSLTVVILFVLLLLRIVLLAERQRSPFTRIFAYCV
                     SAILFFHFFVNVGMAIGLAPVIGIPLPFFSYGGSSIMAFSILIFLLLKLDSERLDILR
                     "
     gene            1054..2067
                     /locus_tag="PK610_09760"
     CDS             1054..2067
                     /locus_tag="PK610_09760"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRJ36262.1"
                     /translation="MDSLTQIVLGAGVGEVILGKKVGNKAMLWGAVAGTIPDLDVLAA
                     LLTNPLQAMIWHRGFMHSIVFCAAFAPLLGWLIHKISKKNNATIKEWSVLCWWALVTH
                     PLLDCFTTWGTQLFWPFDVRITFNGVFVIDPLYTLPFLAFLIVCMRLPRESLRRKKLS
                     RFALYISTAYLFLGLGFRQIAKSTFEARLNEEEIAYRSIDVKPMPFTNLYWECTAEGE
                     AEFYNAHYSLLGKKENIRFRAIAKNHDLDSAFRNHSLYQDLLRISKGYYALETKNDTL
                     LFHDLRFGQTDGISGQIERRSNFVYLLQIDNNHLKFNRRSPAIAQGFSYLCEYFSKVV
                     GAD"
     gene            complement(2135..2794)
                     /gene="msrA"
                     /locus_tag="PK610_09765"
     CDS             complement(2135..2794)
                     /gene="msrA"
                     /locus_tag="PK610_09765"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRJ36263.1"
                     /translation="MKKILFTTASLATVLLFMAGSCGSSMEADNAQIMDEKDTLSSNG
                     LESATLGAGCFWCIEAVYLELKGVKSVVSGYAGGHVDKPTYEEICRGTTGHAEVARIV
                     FDPKVISFDELLEVFWQIHDPTTLNRQGNDVGTQYRSVIFYHNEEQKNSAEKYKMQLD
                     TSGAWDKPIVTEISPLTNFFPAENYHQNYYNNNPNQGYCRFVIAPKMDKFRKVFKEKL
                     K"
     gene            2902..>4032
                     /locus_tag="PK610_09770"
     CDS             2902..>4032
                     /locus_tag="PK610_09770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HRJ36264.1"
                     /translation="MKKLLAALFFLPLAAFGQSEEIEVRSEIKEVTLYLSGAQETRSA
                     RINIPAGNSTLKFIGLPGDIQANSIQVKGVSDFTILSLNFQQNHLTETEEDPELAPLR
                     DSLEALTFAHSLLQAEKSALTEERSMFQNNRRLANNNGLSVDDLNEISEHNADRIRAI
                     DTKLFSLSKTEEKVNKQIQKLNAQVTQVVNGRKRAKGEITVSINAKNKMTNTLVQVSY
                     LTYSAGWKPFYDIRAEEVGGPVKLTYKAHVRQSTGSDWDNVKLTLSTTNPNISNTKPN
                     IPVWYLDFAQDNPIYDVRATSNTGYLISGNTSSGKGAQEQAVLTQNTLNAEFKIASNY
                     SIPSDGVEYVVEIGAYDFKSLYRYSTVPKLRQESYLMARITGW"
BASE COUNT         1147 a          881 c          828 g         1176 t
ORIGIN      
        1 gtcagtttgg aatacaacca tgggagtttg caaaaatcgg agtgagtctt gcactggcca
       61 aatttctaag cacaccgcaa atccgtttcg aagacaccac tacccgattg tacaccgcat
      121 tgttcattgg ttttcctgct gcattaattc tcttacaacc ggatgcagga acagttatcg
      181 tatttatctc ttttattttt gtgctgtaca gagaaggaat gtcgggtaac cttttgcttt
      241 tcggattatt tgtacttgtg ctttccattt tatcgctcat gctgaaagaa agcacaatgg
      301 aattacccat tattggaaca gaagtagcag gacatattgg tctggtgatt attctaacca
      361 tcattgcctt acttgtatta ctgcttatcc gccaattcgt aatgaaacgc ggacgtaaaa
      421 ctgcaattct ggtgttggta ttagcctatt tttcatcgat tgtaattgta tttggaacca
      481 attatgcttt ctacaatgtg atgaagcgtc accagcagga acgaatcgac atcatgttgg
      541 ggctgatcga cgatcctgat ggcaaagcgt acaatatcga ccgatccatg gcagcaattg
      601 gctccggagg attgagcgga aaagggtata tgaaatcgac gctcgccaac agaggtcaaa
      661 aacatgtgcc tatgcaaagc accgatttta ttttctgcac gctaagcgaa gaatggggat
      721 ttgcgggatc cttaaccgtt gtcattttgt ttgtgttatt gttgttgcga attgtgttat
      781 tggcagaacg acaacgatcg ccctttacgc gcatttttgc ctattgtgtt tcagcgatac
      841 ttttcttcca cttttttgtc aatgttggaa tggccatcgg acttgcacct gtaattggta
      901 ttccgcttcc atttttcagt tatggaggtt catcgataat ggcattttca attctgatct
      961 tcctgttatt aaaactggat tccgaacgtt tggatatttt gcgatgaaaa atcctgattg
     1021 catcatctcc aataaacagt acaacaacca ccaatggatt ctctaacaca aatagtttta
     1081 ggcgcaggag ttggagaagt aattcttggc aaaaaagttg ggaacaaagc catgctatgg
     1141 ggtgccgttg caggaaccat tcctgatctg gatgttcttg cagctttgct tacaaatcca
     1201 ttacaagcca tgatctggca tcgtgggttt atgcattcca tcgttttttg tgcagcattt
     1261 gctcctctgt tgggatggct gattcataaa atcagcaaaa aaaataatgc aaccataaag
     1321 gaatggtcgg tattgtgctg gtgggctctg gtaacgcatc ctctactcga ttgttttaca
     1381 acctggggta cgcaactctt ttggcctttc gatgtacgca tcacctttaa tggggtattt
     1441 gtgatcgacc cgctttacac tttgcccttc ttggcctttc tgatcgtttg tatgcgattg
     1501 ccccgtgaat ctttacgcag aaaaaaactg tcgcgttttg ccttgtatat cagcacggcc
     1561 tatctttttc tcggattggg attcaggcaa attgcaaaat ccacattcga ggcgcgacta
     1621 aatgaggagg agatcgcgta tcggtcaatc gatgttaaac ccatgccttt taccaacctg
     1681 tattgggagt gtactgcaga aggagaagct gaattttaca atgcccatta ttctttactg
     1741 ggaaaaaaag agaatatccg cttcagagct attgcaaaaa atcatgattt ggattccgca
     1801 ttcagaaatc attctctata tcaggacctg ctcagaatca gtaaagggta ttacgcgctc
     1861 gaaacaaaaa acgacacttt gttgttccat gacttgaggt ttggacaaac agacggtatc
     1921 tcagggcaaa ttgagcgtcg ttccaacttt gtttacctgc tgcaaattga caacaaccat
     1981 cttaaattca atcgtcggtc acctgcaatt gcacagggat tttcttattt gtgcgagtac
     2041 ttttccaagg tagttggtgc tgattaatgt cgattcaccc gaaacaaaaa attcagcagt
     2101 attcgttgcc gttaaatgca gacttcggtc cgattcattt taatttttcc ttgaacactt
     2161 ttctgaactt atccattttc ggagcgatca caaaacggca atacccctga tttggattat
     2221 tgttgtaata attctgatgg taattttcag caggaaagaa attcgttaat ggcgaaatct
     2281 ctgtaacaat cggcttatcc catgccccgc ttgtatcgag ttgcatttta tatttctctg
     2341 cactgttttt ttgttcttcg ttgtggtaaa aaatcacaga gcggtattgc gtccccacat
     2401 cattcccctg acggtttagc gtggttggat cgtgaatttg ccagaaaact tcgagtaatt
     2461 catcaaagga aatcaccttc ggatcaaaaa cgatacgggc tacttctgca tgaccggttg
     2521 tccccctgca aatctcttcg taagtcggct tatctacatg tccacctgca taaccagaaa
     2581 caacggattt aactcctttc agttccagat aaacggcctc aatacaccaa aaacaacctg
     2641 ctccaagagt tgccgactcc agcccatttg aacttagggt atccttttca tccataattt
     2701 gcgcattatc agcttccatt gaggaaccgc acgatcctgc cataaaaagc agaacagtgg
     2761 ccagggaagc ggttgtaaaa agtatttttt tcataaaatc cacctcaaaa taacaaataa
     2821 caggcgctat tgttgtctca ggcaagaaat atcgtccgtt caggcagaaa tacccttatt
     2881 tttgaagtct attccattag catgaaaaaa ctacttgctg cacttttctt cttgccttta
     2941 gccgcatttg gccaaagtga agaaatagaa gtgcgatcag aaattaagga ggttacattg
     3001 tacctctcag gcgcgcagga aacccgatct gcaaggatca acattccggc cggcaacagc
     3061 actctgaaat ttattggctt acccggcgat attcaggcga attcaattca ggttaaaggg
     3121 gtgtctgatt tcaccatact ttcactgaat tttcagcaaa accacctgac agaaaccgaa
     3181 gaagatcctg aactggctcc attgagggat agtctggaag cgctaacctt tgctcacagc
     3241 cttttgcagg ccgagaaatc tgcgcttacc gaagaacgat ccatgtttca gaataaccgc
     3301 aggcttgcca acaacaacgg cctttcggtg gatgatctca atgaaatttc tgagcataat
     3361 gccgacagga tacgtgcgat tgataccaaa ttattttcat tgagtaaaac cgaagaaaaa
     3421 gtcaacaaac agattcagaa actaaatgca caggttacgc aagtcgtaaa cggaagaaaa
     3481 cgtgcaaaag gagaaataac ggtcagcatc aatgccaaga acaaaatgac caacaccctg
     3541 gtacaggttt cctatttaac ctacagtgca ggatggaaac cattctacga tattcgtgca
     3601 gaagaagtgg gaggccctgt taaactcacg tataaagccc acgttcggca atcgaccggg
     3661 agcgattggg acaatgtaaa acttacgctt tccaccacca atcccaatat cagcaataca
     3721 aaaccgaata tacctgtatg gtatttggat tttgcacagg ataatccgat atatgatgtt
     3781 cgcgctactt ccaatactgg ctacctgatc tcaggaaata cttcctcagg aaaaggagca
     3841 caagaacaag ccgtgcttac ccaaaacaca ttgaatgcag aatttaaaat tgcttcgaat
     3901 tattccattc cttcagacgg tgttgaatat gtagttgaaa ttggagcgta cgatttcaaa
     3961 tcgctttacc gctactccac cgtacctaaa ttacgccagg aatcctattt gatggcccgc
     4021 attacaggat gg
//
LOCUS       DAOMYG010000298         4030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_7927_length_4030_cov_9.369057, whole genome shotgun sequence.
ACCESSION   DAOMYG010000298 DAOMYG010000000
VERSION     DAOMYG010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7927_length_4030_cov_9.369057"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PK610_09775"
     CDS             <1..331
                     /locus_tag="PK610_09775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ36265.1"
                     /translation="CGSTSVAAMAPGCSPPHTYGPTITAVHEVGTPGFLSGYPNPANE
                     LVQLNLEGFIGTVTITLTDMSGRIIRSFNCSGNQSTVMRNELPAGMYFIRAQAGEQVA
                     TTKIVFE"
     gene            complement(422..1750)
                     /locus_tag="PK610_09780"
     CDS             complement(422..1750)
                     /locus_tag="PK610_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36266.1"
                     /translation="MKFSAFLFLLFFTFMQACTPDLERADIDEGRASVSKSVVIGDSY
                     ISGYQNGALSKSGQERSIGALVMRSFKVAGHGAFIQALMPEGDGIGRNAKPWESLYVS
                     ASKLGDRTDCKGVVSLGPLKTLYNPAQAQYFLDPVSANGINDLSIPFLSMSEMVYPAF
                     SPSNEFLERYCASCTNGVPGFITAYQPSFSIVWSGMEDIFRFARNGGYGQSIAPRVTV
                     EGWYNELFTYLTANNAKGVIAGIPDFRTMPFYTLIPWNGADLTLEKADSLTTIYAQAG
                     LNHIVFAEGKNAFVIADVNAPGGYRQLVQGEYITLTVPLDSMKCYFYGLLINPIHDRY
                     VLDSAEVNKLDAATAMFNEVIAQKAAQHDFAFVDMHAYFNRLNQGIYFNGVKYTNEFV
                     SGGFFSLDGFHPTQKGANMIADQFILAINSFFGSTFPTVFCEDCEGLRFP"
     gene            1811..2224
                     /locus_tag="PK610_09785"
     CDS             1811..2224
                     /locus_tag="PK610_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36267.1"
                     /translation="MKTRRLPHAVLMYREGVIIVEFQDNKDIDVKEAREQIAACLQLS
                     ENIPSPVIIIDVHPNTIVSPAAREIFSREVPPGIRISEAIVITNLAKRLIANFYSRYN
                     RPKNPIKVFANFNDAWKWSIEQRDNYKAEHPGTRV"
     gene            2307..3533
                     /gene="sbcD"
                     /locus_tag="PK610_09790"
     CDS             2307..3533
                     /gene="sbcD"
                     /locus_tag="PK610_09790"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HRJ36268.1"
                     /translation="MSLRILHTADWHLGKRLHGADLSKDHLLFIEDLLSIIREREITH
                     LLIAGDVFDHANPSNEALELYYSFIRKLLDTGCALIVTGGNHDSPGVLNAPQSLLKQL
                     RVSVIGCATENPEDEIIELKNSSNQTEAVLAAVPFLRDRDLRRSSEGENYKDRIQAIR
                     EGIKEHYFQLAEICKTNYPGVPAIASGHLYAQGAETSESEREIQIGNQAGIEENTFPE
                     YFAYVALGHIHKAQNVGNSGRICYSGSPVALSFQEKNYKKRVVLIHVENQQVNTEEIE
                     LKKHRDLIVLEGSIESIKKKYASYTNSFTLPSFVELNVVESVFDPLLIREKELLSNEL
                     EQNYSVEVLVSKIQFLSPEKNEADISFTSEDSEELQPLSILKSRINEYPEEEQKELEN
                     TLLSLVEELREKGLLP"
     gene            3530..>4030
                     /locus_tag="PK610_09795"
     CDS             3530..>4030
                     /locus_tag="PK610_09795"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ36269.1"
                     /translation="MRILCIRFENLHSLKGRQEIDLRKAPFSHSGLFAITGPTGAGKS
                     TILDAISLALYNQVPRMPKIISKDSVSKTNALITHGERNAMAEVEYETESGIFRSKWS
                     IAYNRNNNLNDYEMEVAALPSGEILNNKKTEVPLINTRNIGLSYEQFNKAILLSQGEF
                     ARFLKES"
BASE COUNT         1239 a          901 c          848 g         1042 t
ORIGIN      
        1 ctgcggcagc acttcggtcg cagctatggc gcctggctgc tcgccgccgc acacttacgg
       61 accaaccatt acagcggtcc atgaagttgg aacacccggt ttcctgagcg gataccccaa
      121 tcctgctaac gagcttgttc aactcaatct ggaagggttt attggtactg taaccattac
      181 gcttaccgat atgagcggaa gaataataag aagtttcaat tgcagcggaa atcaaagcac
      241 ggtgatgcgc aatgaacttc ctgcaggaat gtactttatc cgtgcgcagg caggtgaaca
      301 ggttgctacc actaaaattg tttttgaata aaaaggattc ttcgctaatc ggcgaaactc
      361 aaaacttaaa taaaaaaggc cactcgaaat gagtggcctt ttctttgcca tgatgtataa
      421 ctcagggaaa gcgaagtccc tcgcaatcct cacaaaatac agtgggaaaa gtactgccga
      481 aaaaagaatt gattgctaaa ataaactgat cagcaatcat atttgctccc ttttgggtgg
      541 gatgaaatcc atcgagcgaa aagaatccgc cacttacaaa ttcgttggtg tattttacac
      601 cattaaaata aattccctga ttcagccgat tgaaataagc atgcatatca acaaaagcaa
      661 aatcgtgttg tgctgctttt tgtgcaatga cctcattgaa cattgcagtg gcggcatcca
      721 gtttatttac ctcagccgaa tcgagcacat agcggtcgtg tataggattg atgagtagtc
      781 cgtaaaaata acacttcatg gaatccagcg gaacagtaag cgtgatatat tcaccctgta
      841 ccaattgacg ataacctccc ggagcattta catcggcgat gacaaatgca ttttttccct
      901 ccgcaaaaac tatgtgattg agtccggctt gtgcatatat ggtagtgagt gaatccgcct
      961 tctcaagggt aagatcagct ccattccatg gaatcaatgt ataaaaaggc atggtgcgga
     1021 aatcaggaat tccggcaatt acccctttcg cattatttgc tgtgaggtaa gtaaacaatt
     1081 cattgtacca accctcaaca gttacacgcg gagcaatgga ctgaccataa cctccattgc
     1141 gtgcaaaacg aaaaatatct tccatacctg accaaacaat actgaagctt ggttgatacg
     1201 ccgtaataaa tccaggtact ccattggtgc agctggcaca ataccgctcc agaaattcat
     1261 tggagggtga aaaagcagga tacaccattt ccgacatcga caagaaggga atactaagat
     1321 catttattcc gttagcgcta accgggtcaa gaaaatattg tgcctgtgca ggattgtaaa
     1381 gcgtctttag cggccccaac gaaacaacgc ctttacaatc ggtgcggtcg cccagtttgc
     1441 ttgccgaaac atacaaggat tcccagggtt tggcattacg tccgattcca tctccttcgg
     1501 gcattaaagc ctgaataaac gcaccatgac ctgctacttt aaacgaacgc ataaccagcg
     1561 ctccaattga acgctcctgt ccgcttttgc tgagtgcgcc attctgataa ccggaaatat
     1621 aactatcgcc aattaccacc gacttcgaca cagaagcgcg tccttcatca atatctgcac
     1681 gttcaagatc gggcgtacaa gcctgcatga aggtgaaaaa aagcaaaaag agaaaagcgg
     1741 agaatttcat acttcgaaaa gtaattaaaa tattgctcgt tcaaaagaaa attctatttt
     1801 cattgttcta atgaagacgc gccgcctgcc ccatgctgtg ttaatgtatc gcgagggcgt
     1861 tattattgtc gaatttcagg acaacaaaga catcgatgta aaagaagcaa gagaacaaat
     1921 tgcagcctgt ttgcagctat cagagaacat cccctctcct gttatcatca tcgatgtgca
     1981 tcccaatact attgtaagtc cggcagcccg agaaatcttt tcaagagaag ttcctccggg
     2041 aatcaggata tctgaggcaa ttgtcattac caacctggca aaacgactta ttgctaactt
     2101 ttactcccgc tacaaccgtc ccaaaaaccc gattaaagtt tttgcgaatt tcaacgatgc
     2161 atggaaatgg tcgatcgaac agcgtgacaa ttacaaggct gaacatcccg gaacacgggt
     2221 ttaacatcct tcgttttttc gggttcataa ctattctctt ctctttttat catctctatc
     2281 ccttctgaat gtaattttat tgaagattgt cgttacgcat tttacatacc gccgactggc
     2341 atctgggcaa gcgattgcat ggtgccgacc taagtaagga tcatcttctt tttattgaag
     2401 atctgctttc cattattcgc gagcgggaga ttacacattt actgatagcc ggagatgtgt
     2461 tcgatcatgc gaatccttcc aacgaagccc tggagcttta ttactccttc attcgcaaac
     2521 tgctggatac aggatgtgca ttgatcgtta ccggaggaaa tcacgattct cccggagtgt
     2581 tgaatgcgcc ccaatcgctg ttaaaacaac ttcgggtgag tgtaattggt tgtgctaccg
     2641 aaaacccgga agatgaaatt attgaactta aaaacagttc taaccaaaca gaagcggtat
     2701 tggcagcggt gccttttttg cgcgacagag atcttcgccg ctcttctgaa ggagaaaatt
     2761 acaaagaccg cattcaggcg atacgtgaag gaataaaaga acactatttt cagcttgcag
     2821 aaatttgcaa aaccaattat ccgggagtac cggcaattgc gtccggacat ttatacgctc
     2881 aaggagcaga aaccagtgag agtgaacgcg agattcaaat cggtaatcag gcgggtattg
     2941 aagaaaacac cttccctgag tatttcgcct acgtagcgct tggacatatc cataaagctc
     3001 aaaacgtagg taacagcgga agaatttgtt actctggatc tcctgtcgca ttaagttttc
     3061 aggagaagaa ttacaaaaag agagttgtgt tgattcatgt tgaaaaccaa caagtgaaca
     3121 cagaagaaat tgaactcaaa aaacaccgcg acctgattgt tttggaaggt agcatagagt
     3181 cgatcaaaaa gaaatatgct tcctacacca attcctttac acttccgtca ttcgtggagc
     3241 tcaatgtagt tgaatctgtt tttgatcctc tgctcattcg cgaaaaagaa ttgcttagta
     3301 atgaattgga gcaaaactat tccgttgagg ttctcgtctc taaaattcag tttttaagcc
     3361 ccgaaaaaaa cgaagcggat atctcgttta cttcagaaga ctcggaagaa ctgcagcctc
     3421 ttagtattct gaaatcaaga ataaatgaat atccggaaga agagcagaaa gaacttgaaa
     3481 acaccttgct ttcacttgtt gaagagcttc gtgagaaagg attgttgcca tgagaattct
     3541 ttgcatacga ttcgaaaatc tgcactcgct gaagggtcgt caggaaattg atttacgaaa
     3601 agcacctttc agtcattccg gactatttgc cattaccggt ccaaccggcg ccggaaaatc
     3661 aacaattctg gatgctattt cgcttgctct ttacaaccaa gttccgcgga tgccgaaaat
     3721 catcagcaaa gattccgttt cgaaaaccaa tgcactgata acacacggtg agcgcaatgc
     3781 catggcggaa gtggaatacg aaaccgaatc cgggattttc agaagcaaat ggtcaatagc
     3841 ctataaccgc aacaacaatc tcaacgatta cgaaatggaa gttgcggcac ttccatcggg
     3901 tgaaattctc aataacaaga aaacagaggt accgctgatc aatacacgga acattggatt
     3961 gagctatgag caattcaata aagccattct tctatcgcag ggagaatttg cccgcttttt
     4021 aaaggaaagt
//
LOCUS       DAOMYG010000299         4016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_7968_length_4016_cov_9.711184, whole genome shotgun sequence.
ACCESSION   DAOMYG010000299 DAOMYG010000000
VERSION     DAOMYG010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7968_length_4016_cov_9.711184"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PK610_09800"
     CDS             <1..507
                     /locus_tag="PK610_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36270.1"
                     /translation="AFALTDPDKIRHMMDQLAVDTLIRKDSSGYYSFSTPFNMDYHFI
                     VQGNVLAMSNTDRYITDIDPLYKGFSNWFYFNSSNFISAIPIDSPFGFMIKGVMEEIS
                     TVHYGYGYSVGTNANNVNFDGKIFYNDKKKHSLIETISFMRAIASMFVGFKTPAEDEE
                     GITSDEEY"
     gene            complement(485..1240)
                     /locus_tag="PK610_09805"
     CDS             complement(485..1240)
                     /locus_tag="PK610_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HRJ36271.1"
                     /translation="MNRQCVLEKKPERIVSLVPSQSELLWDLGLQNELAGITKFCIHP
                     EKMFHTIPRIGGTKQLDLDAIRKINPDLIIGNKEENDRKQIEALEKEFPVWMSDINNL
                     GDAIEMIKSVGMITQRVNEAARIADTIFASFSKSPVISNAPKAIYLIWNKPFMAAGKN
                     TFIDEMIRKAGFTNLISESRYPELTEEQIADLQPEYLLLSSEPYPFEEKHVDYWKKRL
                     PETKTIIVDGEFFSWYGSRLQKAPEYFNTLRQK"
     gene            complement(1258..2622)
                     /locus_tag="PK610_09810"
     CDS             complement(1258..2622)
                     /locus_tag="PK610_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRJ36272.1"
                     /translation="MKVCNNILEAIGHTPMVKLNEITKDIKATVYAKVETFNPGNSIK
                     DRMALKMVEEAEKAGLLKPGGTIIEGTSGNTGMGLAIAAIVKGYKCIFTTTDKQSKEK
                     VDALRAFGAEVIVCPTDVDPEDPRSYYSVSSRLVSEVPNSWKANQYDNPANAKAHYET
                     TGPEIWEQTQGKITHLVVGVGTGGTLCGTGKYLKEKNPNIQILGIDTYGSVFKKYKET
                     GIFDKNEIYPYITEGIGEDFLPENVDFSIIDHFEKVTDRDAAVYTREIVKKEGIWVGN
                     SAGAAIGGLFQMKDRFKEGDVVVVIFHDHGSRYVGKMFNDEWMREKGFLDKSGLTAKD
                     LVGTRNESEVETIEASEKVSVAIERMSKNNYSQMPVTLDRRIVGSLSENTLYKAIVKD
                     PKLKDAMVEVVMEHAFPFVDITTPVDAIAGMLNAETPAVMVKDFKSDKAYIITQYDVI
                     KALC"
     gene            complement(2675..>4016)
                     /locus_tag="PK610_09815"
     CDS             complement(2675..>4016)
                     /locus_tag="PK610_09815"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRJ36273.1"
                     /translation="AYKPSILQSQELDSNKEWRREIQGVLIKGVSGDYNWDFLSENLV
                     EGRLPDIETNVVSQDILISRTLASRLRLHVNDTVSSFFISNNAPRERKLKVCGIYHTG
                     MDELDKKLVFIDLKHLRVINNWGITAMLYVRNDVKDGKLIIEADGRGSGNYLFDFGNG
                     FSTRSTLLLKPGADTTIRVVVASTENTLYGETSDEFASKWIPDTAWLALSYYPKITAA
                     SEYELESNPEVEQAGDTHFVYHHPGLNVIAKMHNSGGSGKYYAGGFEVLLNNWNDLQD
                     SDEFIYKNIGPEFQTKTIRELHQDLFQWLSYLDLNIWVILTLMLLVSLINMCSTLLVL
                     ILERTNMIGLLKALGASNKKLQMVFFYNGVVLIGKGLIIGNIIAIGFALLQWQTGFLS
                     LDQSTYFIDTVPIRMDILSIILLNAGTMIICMVTLLLPTWLVSRISPIKAIRFD"
BASE COUNT         1047 a          848 c          830 g         1291 t
ORIGIN      
        1 gcatttgcat taacagatcc cgataagata cgccatatga tggatcaatt agctgtcgat
       61 acgcttattc gaaaggattc ttcaggatat tattccttct ccactccctt taatatggat
      121 taccatttta tcgttcaagg caatgtgtta gccatgagca ataccgatag atacatcact
      181 gatattgatc cactctacaa aggtttttcc aattggtttt attttaattc aagcaatttc
      241 attagcgcaa ttccaattga ttctcctttt ggttttatga tcaaaggagt tatggaagag
      301 atttcgacag tccattacgg gtacggatat tcggttggaa ccaatgccaa caatgtgaat
      361 ttcgacggta agatctttta caacgacaaa aagaaacatt cactgattga aactatcagt
      421 tttatgcgtg caatcgcatc gatgtttgta ggatttaaaa cgcctgcaga agatgaggag
      481 ggtattactt ctgacgaaga gtattaaaat attcaggcgc tttttgcaga cgcgaaccgt
      541 accaggaaaa aaattcaccg tccacaatta ttgtttttgt ttccggtaat cgttttttcc
      601 agtagtcaac atgtttttcc tcaaaggggt aaggttccga agaaagtaaa aggtattcag
      661 gttgaaggtc tgcaatctgt tcctctgtta gttccggata acgcgattcg cttatcaggt
      721 ttgtaaatcc tgctttacgt atcatttcat caataaaggt gtttttccca gctgccataa
      781 aaggtttatt ccagatcaga taaatggctt ttggagcatt gcttataaca ggtgattttg
      841 aaaaagaagc aaagatggta tctgcaattc gtgcggcctc atttacgcgt tgtgttatca
      901 taccaacact ttttatcatt tcaattgcgt cacccagatt gttgatgtcg ctcatccaaa
      961 ccggaaattc tttttccagc gcttcaattt gtttgcgatc gttttcttcc ttattgccga
     1021 taatgaggtc gggattgatt tttcggatgg catccaaatc cagttgtttg gtgcctccaa
     1081 ttctgggaat tgtgtgaaac attttttcag gatgaatgca gaattttgtt atccccgcca
     1141 actcattttg cagaccaaga tcccacagta attccgactg cgaaggcact aaagaaacaa
     1201 tgcgttccgg ttttttctcc agaacgcatt gtcgattcat ttggtcggtg tagaacatca
     1261 gcagagtgct ttgatcacat cgtattgagt aatgatgtag gctttgtcgc ttttgaaatc
     1321 ttttaccatt acagccggag tttctgcatt gagcattcct gcaatggcat ccacaggtgt
     1381 ggtaatgtca acaaacggaa aagcatgttc cattacaact tctaccattg catctttcag
     1441 cttcggatct tttacaatgg ctttgtagag ggtgttttca cttagagatc caacaattct
     1501 tctgtcgagc gttaccggca tttgcgaata gttgtttttg ctcattcgtt caatggcaac
     1561 actaactttt tcgcttgcct cgatggtttc tacttcactt tcatttcgag taccaacaag
     1621 atcttttgct gtaagtccgg acttgtccag aaatcctttt tcgcgcatcc attcatcgtt
     1681 gaacattttg ccaacataac gcgaaccgtg atcgtggaaa attacaacaa caacatctcc
     1741 ttctttaaat cggtctttca tttggaacaa accaccaatg gcagctccgg ctgaattacc
     1801 aacccatatt ccttcttttt ttacgatctc acgcgtataa acagcagcat cgcggtctgt
     1861 tactttttcg aaatgatcga taatggaaaa atcaacattc tcagggagga aatcttctcc
     1921 gatgccttcg gtgatatagg gataaatctc gtttttatcg aagatacctg tctccttgta
     1981 ttttttaaat actgaaccgt aagtatcaat gccaagaatc tggatgttcg gatttttttc
     2041 ttttaaatat tttcctgttc cgcaaagtgt accgccggta ccaactccaa caaccaggtg
     2101 agtgatcttc ccttgtgttt gttcccagat ctcaggtcct gttgtttcgt aatgcgcctt
     2161 tgcattggcg ggattgtcgt actgattcgc tttccaacta ttcggtactt cactcaccag
     2221 tcgcgatgaa acggaataat acgaacgcgg atcttcggga tcgacatcgg tgggacaaac
     2281 aattacttct gcaccaaatg cgcgcagtgc atctaccttt tctttcgatt gtttatctgt
     2341 tgttgtaaaa atgcatttgt acccttttac aatagccgca atagccaatc ccattccggt
     2401 atttccgctg gttccttcaa taatggttcc gcccggtttt aacagtccgg ctttttcagc
     2461 ttcctcaacc atttttagtg ccatgcggtc cttgatggaa tttcccggat taaaggtttc
     2521 taccttggca tacacagtgg ctttaatgtc cttggtgatt tcgttgagct taaccattgg
     2581 ggtatgacca atagcttcga gaatgttatt acagactttc atgaaatgga tttggtgggt
     2641 caaaagtagg aaaaagttca gacagggtag ggtcttagtc gaagcggatc gctttaatcg
     2701 gactgatcct gctaaccagc catgtaggaa gtaaaagcgt gaccatgcaa atgatcattg
     2761 tgcctgcatt aagcagaatg atggataaaa tatccatgcg gataggtact gtgtcgatga
     2821 aataggtgct ctgatcgagg gaaagaaaac ctgtctgcca ttggagcagg gcaaatccaa
     2881 ttgcaatgat gtttccaata atcagtcctt ttccaatcag caccactccg ttgtagaaaa
     2941 ataccatttg cagttttttg ttgcttgctc caagtgcttt cagtagtccg atcatattgg
     3001 tgcgttccaa aattagtacc agtagtgtgg aacacatatt gatgagcgat accagtaaca
     3061 tcaatgtaag aatgacccag atgttgaggt cgaggtacga taaccattgg aacaaatcct
     3121 gatgaagttc gcgaatggtt ttggtttgaa attccggtcc gatgttttta taaatgaatt
     3181 catctgagtc ttgcaaatca ttccagttgt tgagtaagac ttcaaatcct cctgcatagt
     3241 attttccgct gccacccgaa ttgtgcattt tggcaatgac attcagtcct ggatgatggt
     3301 acacaaaatg ggtgtcgcct gcttgttcta cttcgggatt ggattcgagt tcatattccg
     3361 atgcagcagt aatttttgga taatagctca atgccagcca tgctgtatca ggaatccatt
     3421 tcgaagcaaa ttcatcgctg gtttctccgt aaagtgtgtt ttcagtactc gccacaacca
     3481 cgcgaatagt ggtgtctgct ccgggtttta gcaataaagt agaacgggta ctgaatccgt
     3541 ttccgaaatc gaaaaggtag tttccacttc ctcttccatc ggcttcaata atcagttttc
     3601 cgtcttttac atcattgcgt acatacaaca tggctgttat tccccaattg ttgattacgc
     3661 gcaaatgttt gaggtcgatg aacaccagtt ttttgtccag ttcatccata ccggtgtggt
     3721 aaattccaca gaccttcagc ttgcgttcgc ggggggcgtt gttgctgata aaaaaggagg
     3781 atacggtatc atttacatgc aaacgaagtc gtgatgcaag tgtgcgtgaa ataaggatat
     3841 cctgacttac tacattggtt tcgatatcgg gtagtcgtcc ttcgaccagg ttttcgctga
     3901 ggaaatccca gttgtaatcg cctgaaaccc cttttatcaa tactccctga atttccctgc
     3961 gccattcctt gttgctgtcg agctcctgcg attggagaat ggagggcttg taggca
//
LOCUS       DAOMYG010000300         4003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8000_length_4003_cov_7.304458, whole genome shotgun sequence.
ACCESSION   DAOMYG010000300 DAOMYG010000000
VERSION     DAOMYG010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8000_length_4003_cov_7.304458"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="PK610_09820"
     CDS             <1..998
                     /locus_tag="PK610_09820"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRJ36274.1"
                     /translation="EVPDPKKDSLGFWDMARRSLRRNGFLDSSSLVVDTLVVKALKDL
                     QMQHRMDVDGKPGRQSTWALGLSNYDRFLRAALAMEKWRWKYRSDPQMQFRINIPSYE
                     LNVIRNDSIIYRARVVTGATDHQTPELHATVRYFTLFPYWNVPHSISTEEILPFVKRD
                     TNYLHKHNYRVFDLKRNPVAIADVDWKRLSKDRFPYFIRQEGGPTNSLGLIVFFFPNK
                     HDVYLHDTPMKPLFNREVRNFSHGCMRLQNPFKLGEIVWKDQWPKDTVNADTLRNRAI
                     RFGKETRFALKRRIPIEVDYITVTADSIRNIWFHYDVYARDEKYLEIIRRLRPKH"
     gene            1010..2230
                     /locus_tag="PK610_09825"
     CDS             1010..2230
                     /locus_tag="PK610_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRJ36275.1"
                     /translation="MNTKSISISDYNYDLPEERIAKFPLEQRDASKLLIWKNGTIEES
                     VYRSISNNIPEGSLLFFNETKVIRARMIFKNSTGGKIEVFCLDPYRNRDVSVSMAAQG
                     KVEWNCLIGGLSKWKDDFLQLEHHGIVLEVRRIGTGTDHHPVEFRWNPAGKSFAEILD
                     AIGSLPIPPYLKRETEEIDLNRYQTVYAKNEGSVAAPTAGLHFTPFVFDELKKKNCQS
                     HFVTLHVGAGTFKPVKSATMAEHVMHAEVIDVSLNTISRLAEQQQKMIVAVGTTTTRT
                     LESLYWMGIKLLQSPEIKPDDLFLGQWECYEAYNNDITLKEALSALIDWLRKNGLDRL
                     IAPTSIMIAPGYCFRIVNALVTNFHQPGSTLILLVAAIAGEEWKTIYNYALQNDFRFL
                     SYGDGSLLFVQNNR"
     gene            2310..3902
                     /locus_tag="PK610_09830"
     CDS             2310..3902
                     /locus_tag="PK610_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRJ36276.1"
                     /translation="MSAIEEIAKRRTFGIISHPDAGKTTLTEKLLLFGGAIQTAGAVK
                     SNKIKKTATSDFMEIEKQRGISVATSVMAFNYKGLQINLLDTPGHKDFAEDTYRTLTA
                     VDSVILVIDCANGVEEQTEKLMSVCRMRNTPVILFINKMDREGRDAFDLLDEVEEKLN
                     IHVRPLSWPIGIGATFKGVYNMYEKNLNLFEASKTTIAKEVVQISDLASQELDDIVGE
                     RGAKQLRQDIDLIEGVYDPFDVNTYLKGQVAPVFFGSALNNFGVQELLDAFIRISPTP
                     QGRPTDKRFVEPVEPKFSGFVFKIHANLDPKHRDRIAFLRIVSGKFERNTFYHHVRLD
                     KDFKFANPTSFMASEKNIIDEAYPGDVVGLYDTGNFKIGDTLTEGENMLYQGIPSFSP
                     EIFRELVNNDPMKTKQLEKGIQQLTDEGVAQLFIQQPGNRKIVGTVGELQFEVIQYRL
                     KHEYGASCAFMPVNMFKACWITSDNKAKMDEFVRIKATNIAFDKDHQMVYLAESPFLL
                     KMAEQNYPEIIFHTTSEFKRNG"
BASE COUNT         1212 a          790 c          872 g         1129 t
ORIGIN      
        1 ttgaagttcc cgatccgaaa aaagattcac tgggtttttg ggacatggcg cgtagatctt
       61 tgcggagaaa tggattcctt gattccagct cactggttgt ggatacactt gtagttaaag
      121 cgctcaagga tctccaaatg cagcacagaa tggatgtgga tggaaaaccc ggccgacaat
      181 cgacatgggc gctgggatta tcgaattatg atcggttttt acgtgcagca ttggcaatgg
      241 aaaaatggcg atggaaatac cgctctgacc cgcaaatgca attccgtatc aacattcctt
      301 cctatgaact gaatgtgatt cgaaacgatt ccataatcta tcgagcaagg gtagtaaccg
      361 gagcaaccga tcatcaaaca cccgaattgc atgcaacggt tcgttatttt acactgttcc
      421 cgtattggaa tgttccgcat agtatttcta cagaagaaat tttacctttt gtaaaacgtg
      481 ataccaatta tctgcacaaa cacaattaca gagtttttga tttgaaacga aatcctgtag
      541 caattgcaga tgtcgattgg aaacgattga gcaaggatcg gtttccctat ttcattcgtc
      601 aggaaggtgg tccaaccaat agtttgggac tcatcgtatt tttctttccg aacaaacacg
      661 atgtttacct gcacgacacc ccaatgaaac cgctttttaa cagggaagtc agaaattttt
      721 ctcacggttg tatgcgactg caaaatccgt ttaaacttgg tgagatcgtg tggaaagatc
      781 aatggccgaa ggatacagta aatgcagata cactcaggaa ccgcgccatt cggttcggaa
      841 aggaaacgcg ttttgcattg aagcgaagaa ttccgattga ggtggattat ataaccgtca
      901 ctgccgactc cattcgtaac atctggtttc attacgatgt ttatgcaagg gatgagaagt
      961 atttggaaat tattcgacga ttacgcccga aacactgaag attattccgg tgaatacaaa
     1021 atcaatttca atttcagact ataattacga tttaccggaa gaaagaatcg ccaaatttcc
     1081 gcttgaacaa cgtgatgctt ctaagttgtt aatctggaaa aacggtacaa ttgaagaatc
     1141 cgtatatcgg tcaatatcca ataatattcc tgaaggatct cttttgtttt ttaacgaaac
     1201 aaaggttatt cgtgcacgaa tgatttttaa aaacagcacg ggtggaaaaa tagaagtttt
     1261 ttgtttggat ccttatcgta atcgtgatgt ttctgtttca atggccgctc agggaaaagt
     1321 ggagtggaat tgtttaattg gtggtttgtc caagtggaaa gatgattttc tacaattgga
     1381 acatcacgga attgtacttg aggttcgacg tataggaacc ggtaccgatc atcatccggt
     1441 tgaatttcgt tggaatcctg ccggaaaaag ttttgctgaa attcttgatg ccatcggttc
     1501 actacctatt cctccttact taaagcggga aaccgaagaa attgatctga accgttacca
     1561 aacggtatat gcaaaaaatg aaggatcggt tgctgctcca actgcaggtt tacattttac
     1621 accgtttgta tttgacgaac tcaaaaagaa gaattgtcaa tctcattttg ttacacttca
     1681 tgtaggtgcg ggtacattta aaccggtgaa atctgccacc atggctgaac acgtaatgca
     1741 cgctgaagtg atcgatgtga gtttgaatac catatcacgt cttgcagagc aacaacaaaa
     1801 aatgattgtc gcagtgggaa ctactaccac cagaacactg gagtcgctct actggatggg
     1861 aataaaattg ctacaatcgc cggaaattaa gcccgatgat ttatttcttg gtcagtggga
     1921 atgttacgaa gcttacaata acgatattac cctgaaggaa gctttatctg cattgatcga
     1981 ttggctacga aaaaacgggt tggaccgtct gattgcgcct acctccatta tgattgctcc
     2041 cggatactgt tttaggatag taaacgcttt ggttactaat ttccatcaac ccggcagtac
     2101 gctgattttg cttgtggcag caattgcggg tgaagaatgg aaaaccattt acaattacgc
     2161 cctgcagaac gattttcgtt ttttgagtta tggtgatggg agtttgctat ttgtgcaaaa
     2221 caatagatga aaacctttcc atgcctgaat aattggggga tgaatgcagg atattcttcc
     2281 aactaataat ttcccttact tttgcgccca tgagtgccat tgaagaaatc gccaagcgac
     2341 gtacattcgg aatcatttct cacccggatg caggaaaaac gaccttaact gaaaaactgc
     2401 tcctttttgg aggtgcaatt caaaccgctg gggcagtaaa atcgaacaag atcaaaaaga
     2461 cagcgacttc cgacttcatg gaaatcgaaa aacaacgtgg aatttccgtt gctacttctg
     2521 taatggcatt taactacaaa ggcctgcaaa taaatctgct ggacactccc ggtcacaaag
     2581 atttcgctga agatacctac cgcacactca ctgcagtgga tagtgtaatt ctggtgattg
     2641 actgtgcaaa tggtgttgag gaacaaacag aaaaattgat gagtgtctgc cgaatgcgca
     2701 atacgccggt tattcttttc atcaataaaa tggaccgcga aggccgtgat gcatttgatc
     2761 tgctggatga agtagaggaa aaactaaata ttcatgttcg tcccctgagc tggccaatcg
     2821 gaattggagc tacgttcaaa ggggtgtaca acatgtacga aaagaacctg aatctcttcg
     2881 aggcaagtaa aacaaccatt gcaaaagaag tggttcagat cagtgatctt gccagtcagg
     2941 aattggatga tattgttggc gaacgtggag caaaacaatt gaggcaggat attgatctca
     3001 tagaaggtgt ttatgatccg tttgatgtaa acacttacct caaaggtcag gtagctcctg
     3061 tgtttttcgg atctgctctg aataattttg gtgtgcagga attacttgat gctttcatcc
     3121 gcatctctcc aactccgcag ggacgtccta ccgataaacg ttttgttgaa cctgttgaac
     3181 cgaaattttc aggattcgtt tttaaaattc atgccaacct cgatcccaag cacagagacc
     3241 gtattgcttt tttgagaatt gtcagtggaa aatttgagcg caatactttt taccaccacg
     3301 taagactcga taaggatttc aaatttgcca atccaaccag ttttatggct tccgaaaaaa
     3361 acatcatcga tgaagcctat ccgggagatg ttgttggttt gtatgatacc ggaaatttca
     3421 agattggtga taccctcacc gagggagaaa atatgttgta tcagggaatc ccaagtttct
     3481 cgcctgaaat attcagggaa ttggtgaata atgatccaat gaaaaccaag cagcttgaaa
     3541 aaggtattca gcagctcacg gatgaaggtg ttgcgcagtt gtttattcaa cagcccggaa
     3601 acagaaaaat tgttggtaca gttggcgaac ttcagtttga agtcatacaa taccgactca
     3661 agcacgaata cggtgcttcc tgtgctttca tgccggtgaa tatgtttaaa gcgtgttgga
     3721 ttacatccga caacaaagca aaaatggatg agtttgttag gatcaaggca accaatatcg
     3781 ccttcgataa agatcatcaa atggtttacc ttgcagaatc acctttcctc ctgaaaatgg
     3841 ctgaacagaa ttatcctgaa atcattttcc ataccaccag cgagtttaag cggaatgggt
     3901 aaatatttga aaattgccaa ttaaccaata agccaattac caattattca atttctggaa
     3961 cctaatccta aagcgaaatc gactaccggg agctttctgc ttt
//
LOCUS       DAOMYG010000301         4002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8001_length_4002_cov_8.828224, whole genome shotgun sequence.
ACCESSION   DAOMYG010000301 DAOMYG010000000
VERSION     DAOMYG010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8001_length_4002_cov_8.828224"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="PK610_09835"
     CDS             complement(<1..121)
                     /locus_tag="PK610_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRJ36277.1"
                     /translation="MNKAEMIDAIAAESGLSKADAKRAVDSFVNVTTKALKKGD"
     gene            complement(293..694)
                     /locus_tag="PK610_09840"
     CDS             complement(293..694)
                     /locus_tag="PK610_09840"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRJ36278.1"
                     /translation="MKTLLLLFSAVLFSASLNAQAYDASKITPPAEYENIHVHKVAGD
                     SLTSTFIIFIKQNVAMHKHESHSENVIVLEGSGTMTLGSDRFEIKAGDIIFIPVNTWH
                     SVIVNSIVPLKVISVQSPEFDGTDRVIYKNQ"
     gene            985..3312
                     /locus_tag="PK610_09845"
     CDS             985..3312
                     /locus_tag="PK610_09845"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ36279.1"
                     /translation="MRGILLLLFTACVCSIAAQQTVKGKVTDAELGRPIPGANVVLLH
                     TEPLRGTVTDANGYFRFENVPLGRQDIRVTMIGYEPLTIPNVLVTSGKEVELNVKLQE
                     SVTEMEEVVVQAEGEKGETINQMTIISARSFSVEESKRYAGSFDDPARMAQAYAGVAS
                     SNDASNEIVIRGNSPRGLLWRMEGVEIPNPNHFSDQGSSGGAISMLSSNMMTNSDFFT
                     GAFPAEYGNALSGVFDIHLRKGNSDRREYAFQLGVLGTDIALEGPFKKGYTGSYLFNY
                     RYSTLSLFNKVGINLAGDAVPVFQDLCLNVVLPAGKAGTFTLFGLGGNSLVKDDWKSD
                     SLHFQDKFSVWMGVGGITHTLFLNSTTYLKTIVSLSGSANQYFSQVFDTLGNYRYDEY
                     DQNNRQNAVRASVTLTKKSGPKHLFRMGMIGTKQSFNFRDRFYDQPTDAMIVYLDQKG
                     NSGYVQGFFNWKYRPKEKWTMMSGVHYLHFLLNNRFALEPRFGLKYDIRPGTSLQLGA
                     GLHSRMEDLSVYQAGLDLPGGARHLPNKDLGFTRTIHFVAGYSQMVRENLQVRPEIYF
                     QHLFDVPIIDKPNSYFSAINFGSGYTLDTLSNKGSGRNIGLDLTVERFFADRWFLLFT
                     GSLYQSTYIAGDQKRYNTRYNGNFANSFALGKEWNVGKSSKRNILSVSMRGTYAGGRR
                     YTPILLAESVLAGRELADQSRVFSSRLKDYFRADLQISYKRNNKKTTSTWKIDAQNVS
                     NRQNIFQYYFDVKTGDIRTIYQLGFVPVISYKLEF"
     gene            3365..4000
                     /locus_tag="PK610_09850"
     CDS             3365..4000
                     /locus_tag="PK610_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HRJ36280.1"
                     /translation="MNTTSGNSNPLVIQKLPLMEHFYTIQGEGAFSGRAAYFLRLGGC
                     DVGCVWCDVKESWNAANHPLVSVGEMVKYVSDSGADYCVITGGEPAIYDLLALTTALN
                     EKGIQTAIETSGAYPLSGNWHHVCFSPKKFKAPVESIYPLANELKVIVFNKSDFVFAE
                     EHASRVNEACLLFLQPEWEKEKEMLPLIIEYIKSNPRWRISLQTHKYMNIP"
BASE COUNT         1074 a          825 c          949 g         1154 t
ORIGIN      
        1 ggtcaccttt tttaagagct ttagtagtta cgtttacgaa gctgtcaaca gcgcgcttcg
       61 catcagcctt tgaaagacca gattctgccg caatggcgtc aatcatttct gccttgttca
      121 tgatgttaga tttaaaggtt agtttttgtt tgtctgaaac aaatatatgc tggaaggctt
      181 accagtcaag gctttcacgt catatcatct gaattctgtt gaaaaatcgg ggcttttgtt
      241 gaaaagcctt cgcttaaacc cagtgttttc ggggctttca gcgtgtttgg gattactgat
      301 ttttataaat cacccgatct gttccatcaa attcgggtga ttgaacggaa attactttga
      361 gcggaacaat ggaattaaca ataaccgaat gccaggtatt tacgggaatg aaaatgatat
      421 cgcctgcttt tatttcaaaa cgatcgcttc ctaatgtcat tgtacccgat ccttccagca
      481 caattacatt ttcactatga ctctcatgtt tatgcatcgc cacgttttgc ttgatgaaaa
      541 tgataaaggt gctggtcagt gaatcgccgg ccactttgtg tacatgaatg ttttcgtatt
      601 cggcgggagg agtgattttg gaagcatcgt atgcctgcgc attaaggctt gcggaaaaga
      661 gcactgcaga aaagaggagt aggagtgttt tcatgatagc gaaatgtagt aaatttattt
      721 ggtggtgttt tttgtgggga actctataga ggaagaaatc agaaaaaaaa atagtaagtt
      781 atattcctag gtggtctaaa gttcctaatt caaatttaat tgacactcct ttcgattatt
      841 tttctttaac ttatgcttta ctaatacaat atttaatctt ctaataattt tcccattaaa
      901 tttctatttt gattactctc cccaaaaaaa ctatcttgtt cctgcaacct tttgaaagaa
      961 aactgtcagt agagaaaaaa gataatgaga ggaattctgt tactgctatt tacagcatgt
     1021 gtttgttcga tcgctgcaca gcaaacggtg aaaggaaagg ttaccgatgc ggaactgggg
     1081 cgtcccattc ctggtgctaa tgtagtattg ttgcacaccg aacccttgcg gggaaccgtt
     1141 actgatgcca acggctattt ccgtttcgaa aacgttccct tgggcaggca ggatatccgc
     1201 gtcacgatga tcggttacga accgcttacc attcccaatg tgttggtgac atccggaaag
     1261 gaagtcgaac tcaacgtaaa attgcaggag tcggtcaccg aaatggaaga ggtggtggtg
     1321 caggccgagg gagaaaaagg ggagaccatc aatcagatga ccatcatttc tgcccgcagt
     1381 ttttcggtgg aagaaagcaa acgctatgca ggaagttttg atgatcctgc acgaatggct
     1441 caggcctatg cgggagtggc ttcatccaac gatgccagca atgaaattgt gatccgtgga
     1501 aattctccaa gaggattgtt gtggcgcatg gagggtgtgg aaattcctaa tcccaaccat
     1561 ttttccgacc agggatcatc gggtggtgcc atcagtatgc tcagcagtaa catgatgacg
     1621 aactccgatt tttttaccgg cgcttttcct gctgaatatg ggaatgcgct ctcaggggtc
     1681 tttgatattc atttgcgaaa aggaaactcc gaccgacgcg aatatgcgtt tcagttgggc
     1741 gtactgggaa cagacattgc acttgaaggt ccgtttaaaa aaggatacac cggcagttat
     1801 cttttcaatt acaggtattc cactctttcc ttgttcaata aggtaggcat caatctggca
     1861 ggagacgctg ttccggtgtt tcaggatctg tgcctgaatg tggttttgcc cgcaggtaaa
     1921 gcaggaacat tcaccttgtt tggtctggga ggaaatagtt tggtaaagga cgactggaaa
     1981 agcgattcgc tccattttca ggataaattt tcggtgtgga tgggcgttgg aggaatcaca
     2041 cacactttgt ttttgaacag tacaacctac ctgaagacca tcgtttccct gagtggttct
     2101 gccaatcagt atttttcgca ggtgttcgat acgttgggga attaccgtta cgatgaatac
     2161 gatcagaaca accgacaaaa tgcggtacgc gcatccgtaa cactcaccaa aaaatccggt
     2221 ccgaagcatt tgttccgaat gggaatgatc ggaacaaaac aatccttcaa tttccgcgac
     2281 cgcttttacg atcagcccac cgatgctatg atcgtttacc tcgatcagaa agggaacagc
     2341 ggatatgttc agggcttctt caactggaaa taccgtccga aggaaaaatg gaccatgatg
     2401 tcgggtgtac attacctgca ttttctcctc aacaaccgat ttgcccttga gcctcgtttc
     2461 ggtttgaaat acgatatacg tccgggtaca tccctgcaat tgggagcagg tttgcacagt
     2521 cgcatggaag acctttccgt gtaccaggca ggccttgatc ttccgggtgg agcacggcat
     2581 cttcccaata aggatcttgg atttacccgc accattcatt ttgtggcggg ctattcgcaa
     2641 atggtccgcg aaaacctgca ggtccgcccc gagatttatt tccagcacct gttcgatgtg
     2701 cctatcattg acaaaccgaa cagttatttt tctgccatca attttggcag tggttatacg
     2761 ctcgatacgc tcagcaataa aggaagcggt cgcaatatcg ggttggatct aaccgtagaa
     2821 cgtttttttg cggatcgctg gtttttgttg tttaccggtt cgctgtatca gtcgacctac
     2881 atcgctggcg atcagaagcg gtacaatacc cgttacaacg gaaatttcgc caacagtttt
     2941 gcgcttggaa aagagtggaa cgtcggcaaa tcatcgaagc gaaacatcct ttctgtgagc
     3001 atgcggggaa cctatgccgg agggaggaga tatactccga tccttttggc tgaatctgtt
     3061 ttggcaggca gggaattggc agatcagagt cgggtgtttt cttcgcggtt aaaggattat
     3121 ttccgtgccg acctgcagat cagttacaag cgcaacaaca aaaagaccac ctctacctgg
     3181 aaaatcgatg ctcagaacgt gagcaaccgt cagaacattt ttcagtatta tttcgatgta
     3241 aaaacaggag atatccgaac catttaccag ttgggatttg tgccggtaat cagctataag
     3301 ctcgaattct gagggaaata gcatataatc cctctgctga gcgatatttc gtatttttgc
     3361 aggaatgaac actacatccg gaaattccaa tccccttgtt attcagaaac ttcctctgat
     3421 ggagcatttt tacactattc agggtgaggg tgcgtttagt ggtcgggctg cttattttct
     3481 gcggcttggt ggttgtgatg ttggatgtgt ctggtgcgat gtaaaggaga gctggaacgc
     3541 tgcaaaccat ccactggtat cggtgggaga gatggttaaa tatgtaagcg attcgggcgc
     3601 agattattgc gttattaccg gaggtgaacc tgcaatttat gatttgcttg cacttacaac
     3661 agcgttaaac gaaaaaggaa ttcaaactgc tattgaaact tccggtgcat atcccttgag
     3721 cggaaactgg catcacgttt gcttctctcc taaaaagttt aaagctccgg tggaaagtat
     3781 ttatcctttg gcaaacgaat tgaaggtgat tgtgttcaat aagagcgatt tcgtttttgc
     3841 agaagaacat gcttcacggg taaatgaagc ttgtttgctt tttcttcaac cggaatggga
     3901 aaaggaaaaa gaaatgttgc ctctgataat tgaatacatc aaatctaatc cgcgctggag
     3961 aatttctctt caaacgcaca aatacatgaa cattccctga ct
//
LOCUS       DAOMYG010000302         3970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8104_length_3970_cov_8.403321, whole genome shotgun sequence.
ACCESSION   DAOMYG010000302 DAOMYG010000000
VERSION     DAOMYG010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8104_length_3970_cov_8.403321"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /gene="dnaN"
                     /locus_tag="PK610_09855"
     CDS             <1..981
                     /gene="dnaN"
                     /locus_tag="PK610_09855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRJ36281.1"
                     /translation="PIGFGPGPGGGVEAKEDGNICIPAKLLIETLKNFPELPLTFSID
                     ARNFSIEISSDNGKFKLTGHNASEFPKVPPIDAASSVSMPSSVLLNAIVKTIFATGND
                     DLRPVMSGVFCQLSSNDFTFVATDAHKLVRFRRTDLKSEKDANFIIPKKPLNLLKSAL
                     GSATGEVKVEFNQTNALFTFDHHTLICRLIDGKYPNYEAVIPKQNPNKLTIDRSTFLS
                     SLKRVSIFSNKTTHQVRLKIAGSELAVSAEDLDYANEAMERLTCSYEGEDMEIGFNSK
                     FLIEMLNNLQCNDVVLEMSAPNRAGILLPAEGDNKDENILMLVMPIMLNN"
     gene            complement(1035..1667)
                     /locus_tag="PK610_09860"
     CDS             complement(1035..1667)
                     /locus_tag="PK610_09860"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRJ36282.1"
                     /translation="MDAFEETRISWNKVAVLYQEKFFDLKLYQNGYDRFCELLIGDEK
                     KVLDVGSGPGVIARYLLDKNPKLTVTGIDYAAGMVELAKRNCPEADFLEMDVRQMSKL
                     GERYIGIAAGFIVPYLSREELQSFFRNCFNLLNSEGVFYFSFVESEEEWSGFITGSTD
                     LRVYFYYYPTILIEKFLADAGFKDIEWEKCIYEKPGGKTEVHAMVVCKRP"
     gene            1796..2803
                     /locus_tag="PK610_09865"
     CDS             1796..2803
                     /locus_tag="PK610_09865"
                     /inference="COORDINATES: protein motif:HMM:NF020416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HRJ36283.1"
                     /translation="MKASSCFLPLFFLATQLNASQNSNGFRHFSIQHKKHGPINYHIS
                     SPEFEKSKPVLLYLDGSGHYPLLMLTPKGKGSTIPFDTDSLSRMYHLVVISKPGIPFE
                     DSVQYGGPIGIDYPEPEEYAKRLSFDWRYETALAVLNELPKKISVANQFYGILGISEG
                     AQVAPSVAASFKPCKALITIGGNAVNQFYDFTTNARLMAERGEISYEDAEYIVDSLHQ
                     VYREIYAEPFSTTKRWYGHTYLRWASFCSREPLNEYLKLNIPVYVAYGSADENTILNI
                     DLLRHAYNRLNKTNLTMKTYPGLDHFFRDKKNTDQSKGNPYKEVIKNCMNWLMNQLNG
                     K"
     gene            complement(2804..3526)
                     /locus_tag="PK610_09870"
     CDS             complement(2804..3526)
                     /locus_tag="PK610_09870"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRJ36284.1"
                     /translation="MHKQERRYLLRPLIIPVILVGIAWTVWWLVANAAVEFGVRPQTL
                     KGLAGIFFSPFIHDPKDVSHLINNSLPLLFLGWALFYFYRELSWKVLAWIWFLSGFWV
                     WIAVQDESVHIGASGIVYGLASFLFFSGVIRRQRNLMGLTLIVSFFYGGMVWGVFPYD
                     LRISWQSHLFGGIAGLILAIVYRKKGWQREKYAWEDEVVTENFSQLNERHEAVLHGKI
                     KDPNEIDEDPPFTINYEFVSKE"
     gene            complement(3625..>3970)
                     /locus_tag="PK610_09875"
     CDS             complement(3625..>3970)
                     /locus_tag="PK610_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HRJ36285.1"
                     /translation="VYVDAKAFYPHIPAHEYPGQTLACALYREGGIRSCEIGSVMFGK
                     YDENGKLITAPMELVRLAIPRRVYTQSHIEYVLEVFELLNKQKNSEKGMRIIKEPKFL
                     RHFTSHFEYNSR"
BASE COUNT         1194 a          940 c          739 g         1097 t
ORIGIN      
        1 ccgattggat tcggtccagg tccgggtggc ggggttgaag cgaaggaaga cggaaatatt
       61 tgtattcctg ccaaactttt gattgaaacg ctgaagaact ttccggagtt gccactaacc
      121 ttttcgatcg acgcccgcaa tttctcgatt gagatttcca gcgataacgg taaattcaaa
      181 ctaacaggcc ataacgccag cgagtttccc aaagtaccac cgattgatgc agcttcctct
      241 gtaagcatgc cttcatctgt gctcctgaat gccattgtaa aaaccatttt cgcaacaggt
      301 aatgacgatc ttcgtccggt aatgagcggt gtattttgcc agctttcttc aaacgatttc
      361 acctttgttg caaccgatgc acacaaactc gttcgtttcc gccgtactga tcttaaatca
      421 gaaaaggatg ccaacttcat catcccgaaa aaaccactca atctcttaaa gagcgcattg
      481 ggtagtgcaa ccggagaggt taaagttgaa ttcaaccaga ccaacgcctt gttcacattc
      541 gatcaccata cattgatttg ccgcttgatt gacgggaaat accccaatta cgaggcggta
      601 attcccaaac aaaacccgaa caagctgaca atcgaccgtt ctacattttt gagttcactt
      661 aaacgtgtat ccatcttctc caataaaact acgcaccagg tccgtttaaa aattgccggt
      721 agtgagcttg ctgtatcagc cgaagatttg gattacgcca acgaagctat ggaacgttta
      781 acatgctctt acgaaggtga agacatggaa atcggattta attcaaagtt tctcattgaa
      841 atgctgaaca atcttcagtg caatgatgta gttcttgaaa tgagtgcccc caaccgcgca
      901 ggaatacttt tacctgccga aggagataat aaagacgaaa acatactgat gttggtaatg
      961 cccattatgc tgaacaattg attttttatt gccatgatgc aattgaggga gcgggcgact
     1021 gctccctttt ttgtttatgg ccgtttgcaa acaaccattg catgcacttc ggttttacct
     1081 cccggttttt cataaataca tttctcccat tcaatatcct taaaacctgc atccgccaga
     1141 aatttctcaa tgaggatagt tggataataa taaaagtaaa cccgcaaatc ggtgcttccg
     1201 gttatgaatc cagaccactc ctcttcactc tcaacaaaac tgaaataaaa cactccttca
     1261 ctattcagga ggttgaaaca attcctaaag aatgactgca gctcttcgcg ggagagatac
     1321 ggaacaataa accctgcggc aattccaata tagcgttctc caagtttgga catttgccta
     1381 acatccattt ccagaaaatc agcttcaggg caattcctct ttgctaattc gaccataccc
     1441 gcagcataat caattccggt taccgttagc ttcggatttt tgtcgagcaa atacctggca
     1501 atgacgccag gtccactgcc aacatcgagc actttttttt catcgccaat aagaagctca
     1561 caaaagcgat cgtagccgtt ctggtaaagt ttgaggtcaa agaatttctc ctggtacaat
     1621 accgcaacct tgttccatga aatacgtgtt tcttcaaagg cgtccatgat ctttcacatt
     1681 atgtttgcaa tttaagttat taaaaacacc atccattttt gatttattat aatctattaa
     1741 gttttcttca catttcacgt aaggacaggc aagtagtttt gtgaaaaatt cagctatgaa
     1801 agcatcctcc tgttttcttc ccctattctt tcttgcaact caactgaatg catctcagaa
     1861 ttcaaacgga ttccgccact tttccattca gcataaaaaa catggtccta tcaactacca
     1921 catatcatca ccggaattcg aaaaatcaaa accggtattg ctttatctcg atggttccgg
     1981 gcattatcca ttattgatgt taacacccaa aggcaaaggc tcaaccattc ctttcgatac
     2041 cgattcgttg agcagaatgt atcatcttgt tgttatcagc aaaccgggta ttccatttga
     2101 agattccgta caatacggtg gtccgatcgg tattgactat cctgaaccgg aagaatacgc
     2161 caaacgactc agtttcgact ggagatatga aactgcattg gcagtattaa atgagctgcc
     2221 aaaaaaaatc agcgtcgcaa atcagtttta cggtattctg ggcatttctg aaggcgcaca
     2281 ggttgctccc tctgttgccg catcgttcaa accatgcaag gcactcatta ctattggcgg
     2341 aaatgccgtg aatcagtttt atgatttcac caccaacgca cgactcatgg ccgaacgtgg
     2401 agagatcagt tatgaagatg ccgaatatat cgtggattcc ctgcatcagg tatatcgtga
     2461 aatatatgca gagccgttta gtaccacaaa acgatggtat ggacacacct atttgcgctg
     2521 ggcatcattt tgttcgcgag aaccattgaa tgaatacctg aaactcaaca ttcccgttta
     2581 cgttgcctac ggaagcgcag atgaaaacac tatcctgaac atcgacctcc tccgtcatgc
     2641 ctacaaccga ctcaacaaaa ctaatcttac gatgaaaaca tatcccgggc ttgatcattt
     2701 tttccgcgac aaaaaaaata ctgatcagtc caagggaaat ccttacaagg aagtcataaa
     2761 aaactgtatg aactggttga tgaatcagct aaatggaaaa tgattactct ttcgatacaa
     2821 actcgtagtt aatggtaaat ggaggatctt catcaatttc atttggatct tttatttttc
     2881 catgcaaaac cgcttcgtgg cgctcgttga gttgagagaa attttcggta acaacttcat
     2941 cttcccatgc atatttttca cgctgccatc cttttttgcg gtagacaatg gcgagaatta
     3001 atcctgcaat tcctccgaac aaatgactct gccaggagat tcgcaaatca tatgggaaaa
     3061 caccccacac cattccacca taaaagaaag atacgatcaa tgttaatccc atcagatttc
     3121 gttggcgacg aattacccca ctgaaaaaca aaaaagatgc gagtccatag acaataccgc
     3181 ttgcaccaat atgtacactt tcatcctgca cagcaatcca tacccagaaa ccgctcaaaa
     3241 accatatcca cgccaatact ttccaggaaa gctcccggta gaaatagaac aaagcccaac
     3301 cgagaaataa cagtggaagt gagttgttga tgagatgcga aacatctttg ggatcatgaa
     3361 taaacggtga aaaaaatatt cctgccaaac ctttaagcgt ttgtggccgc acaccgaatt
     3421 caactgcagc atttgccacc aaccaccaaa ccgtccatgc tattccaacc agaataactg
     3481 gaatgatcaa gggacgtaat aaataccggc gttcttgctt atgcatgcct tgttttctcc
     3541 aaatactatt cctttcctca aactctgcca ataagactgt tttaatgaca gaatgggtta
     3601 gttttcaatc gttcacaaaa tgcttcagcg gctattgtat tcgaaatgtg aagtaaaatg
     3661 gcgtaaaaat ttcggctctt tgataatacg catacctttt tcactattct tttgtttgtt
     3721 cagcaattcg aaaacttcga gcacatattc gatatgactc tgggtataaa cacgccgggg
     3781 aatagccaaa cgaaccaact ccattggagc agtaatcagt tttccattct catcgtattt
     3841 tccaaacatc accgaaccaa tttcgcagga acggattcca ccctcacggt agagtgcaca
     3901 ggccagtgtt tgtccgggat attcgtgtgc gggaatgtgc ggataaaacg cctttgcatc
     3961 cacatacacg
//
LOCUS       DAOMYG010000303         3948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8159_length_3948_cov_8.511688, whole genome shotgun sequence.
ACCESSION   DAOMYG010000303 DAOMYG010000000
VERSION     DAOMYG010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8159_length_3948_cov_8.511688"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..640
                     /locus_tag="PK610_09880"
     CDS             47..640
                     /locus_tag="PK610_09880"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRJ36286.1"
                     /translation="MKRSTIILLSVAGVLFILVMWVRSMYNGIIETEGTVDQKFADIQ
                     TQYQRRFDLIPQLVATVQMNAENEKEIITKVTQYRAGITDAKNAGDIEKMEPMARDIR
                     TAINLQVEAYPNLKSPDAFRDLQAQLEGTENRIAKARSDYNEAVRGYNVRIKRFPTNM
                     FNAMFGFEPRESFKADEGSDKAVDVREEFGKGKNAAE"
     gene            627..1061
                     /locus_tag="PK610_09885"
     CDS             627..1061
                     /locus_tag="PK610_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRJ36287.1"
                     /translation="MPRNNFSDADILRIEQSIAEAEKITSGEIRIFIDNRCKEDVLDR
                     AAWIFHEIGMDATEERNGVLIYIALKDHKFAIIGDAGINEKVPKGFWDAIRDEMKNLF
                     RDGKYSEAIVTGILKAGAQLSHHFPYKENDTNELSNEMLFGK"
     gene            1058..1861
                     /locus_tag="PK610_09890"
     CDS             1058..1861
                     /locus_tag="PK610_09890"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRJ36288.1"
                     /translation="MKRILLFLLPILLFSSCEEKKSCSIPDKPVPNRLVNDFAGILSE
                     NDTRSLEQTLRDYSVSTTTDIAIVTTNELCQYTPAEYAFKIIDNWGIGKADKDNGLVI
                     LVVPKESSEDGKGHCFIATGRGLEGAVPDAVAKRIVEDEMIPHFKSGHYSSGIHAGVV
                     AVMTRTSSEFVPQPISGLSKSKAKKKSEKFATIIALIGIVFFWFLISLKRTSDYSRRN
                     HVPFWTGFWIGNSMGRHGGSWGGFSGGGGGGWGGFGGGSSGGGGAGGSW"
     gene            complement(1925..2359)
                     /locus_tag="PK610_09895"
     CDS             complement(1925..2359)
                     /locus_tag="PK610_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36289.1"
                     /translation="MSVTGSDPYKIVPPAISSGSVYLFTTDSGVEYEVRFARKKDNLL
                     HATVAFGVLNEEFGGEEYALTNRGEVYRVMSTIVTIVRNYMAEHPNLRLVEFTGEPKE
                     NESPDEATKRLLLYNRYLKFIFDESWTFRQMGNRMVVERKEI"
     gene            2419..3174
                     /locus_tag="PK610_09900"
     CDS             2419..3174
                     /locus_tag="PK610_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HRJ36290.1"
                     /translation="MAKFGKWLGGGLGWALGGPIGAVFGFAVGSMVDATSFEKNEEIE
                     GQFRQTRERYRHHTTPGDFTASLLILTAVVMKADGKIMKSELDYVRNFFLRQFGEYRT
                     QQILKELQQILKKEIPVQEVCMEIRHYMEHPMRLQLIHYLFGIAAADGSVDSAEQRVI
                     LQIAGYLGVNSRDFESIQAMFVRDTVSAYKILEIESSASDEEVKKAYRKMAVRYHPDK
                     VASLGEEHQKAAQEKFQKVQEAYENIRKERGMN"
     gene            3405..3779
                     /locus_tag="PK610_09905"
     CDS             3405..3779
                     /locus_tag="PK610_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36291.1"
                     /translation="MESRKFSEETLTAVVKKKNILLVIEYCLENKIEFVVTPGAGSDD
                     FLIGFVIKELGAAVALGMSLKDLKIDLQGLPGFISVNQIRSKKGNEASPAKGSKNESR
                     DMSDELEIPTMENALKFDLENA"
BASE COUNT         1233 a          809 c          866 g         1040 t
ORIGIN      
        1 aaattacgta gtacacctca accaataatc taatcttaaa tagaaaatga aacgttcaac
       61 cattatttta ttatccgtag caggagtatt attcattctt gtaatgtggg tccgttccat
      121 gtacaatgga attattgaaa ctgaaggaac agttgaccag aaattcgctg acatccagac
      181 acagtatcag cgtcgttttg atcttatacc tcaattggtg gcgactgtgc agatgaatgc
      241 ggaaaacgaa aaagaaatta ttaccaaggt tacccagtac cgtgccggaa tcaccgatgc
      301 aaaaaatgca ggtgatatcg aaaaaatgga accgatggca cgtgatattc gcaccgctat
      361 caatttgcag gtagaagcct atccaaatct gaaaagtccg gatgcatttc gcgaccttca
      421 ggcacaactt gaaggaacag aaaatcgtat tgccaaagca agaagcgatt acaacgaagc
      481 tgtgcgtgga tacaatgttc gaatcaaacg attccctacc aacatgttca atgctatgtt
      541 tggttttgag ccaagggaaa gcttcaaagc tgatgaagga tccgataaag ctgtagatgt
      601 ccgggaagaa ttcggaaagg ggaaaaatgc cgcggaataa tttttcggat gcggatattc
      661 tccgcataga acaaagcata gccgaagcag aaaaaatcac ctccggagag attcgcattt
      721 tcatagacaa ccgttgtaaa gaagatgtcc ttgatcgcgc cgcatggatc tttcacgaga
      781 ttggtatgga tgccaccgaa gaacgaaacg gagttctgat ttatatagca ttgaaggacc
      841 ataaattcgc aattatcggt gatgccggaa tcaacgaaaa agttccgaaa ggtttctggg
      901 atgctatccg cgatgagatg aaaaatcttt ttagggatgg caaatattct gaagcgattg
      961 taacaggaat cctgaaagca ggagcacaac tcagtcatca tttcccttac aaggaaaacg
     1021 acacgaacga attgtcgaac gaaatgctct ttgggaaatg aaacgcattt tactttttct
     1081 tttaccgatt ctgctatttt cctcctgcga ggagaaaaaa tcctgcagca ttccagacaa
     1141 acctgttccc aacagattgg ttaatgattt tgccggaatt ctaagtgaaa acgacacccg
     1201 ttcacttgag caaactttaa gagattattc tgtaagcacc actaccgaca tcgccattgt
     1261 aacgaccaac gaattatgtc agtacacccc ggcagaatat gcattcaaaa taatcgacaa
     1321 ctggggaatt gggaaagccg acaaagacaa cggacttgta attcttgttg taccgaaaga
     1381 aagttcggag gatggaaagg gacattgctt tatagcaacg ggacgcggac ttgaaggcgc
     1441 tgttccggat gctgttgcaa agcgaattgt ggaagatgaa atgattcctc attttaaatc
     1501 ggggcattac agttccggaa tccatgcagg tgttgtagct gtgatgacac gtacatcttc
     1561 ggaatttgtt ccgcaaccca tttccggatt atcgaagtcg aaagcaaaga aaaaatcgga
     1621 gaaattcgcg acaataattg cattaattgg cattgttttc ttttggtttc ttatttcatt
     1681 gaagcgaaca agcgattatt cccgaagaaa tcatgtaccc ttctggacag gtttctggat
     1741 aggaaattca atgggccgac acggtggaag ctggggaggc ttttccggcg gcggcggcgg
     1801 cggttggggc ggttttggag gcggcagcag cggtggcggt ggcgcaggcg gatcgtggta
     1861 ataattattt caaacgtgaa cacaacacac atttactttt cactattcac cactcaccaa
     1921 aaaatcaaat ctccttccgc tcgacaacca tacgattgcc catttgtcgg aatgtccagg
     1981 attcatcaaa aataaatttg agatatctgt tataaagcaa gagacgcttg gtcgcctcat
     2041 cgggagattc attttctttt ggttcaccgg taaattccac caacctgaga ttcggatgct
     2101 ctgccatgta gtttcttaca atggtaacta tggtcgacat caccctataa acttcacctc
     2161 tgttagtgag cgcatattct tcaccaccaa attcttcatt caatacacca aatgctacag
     2221 tagcatgcag gagattgtct tttttacggg cgaaacgcac ttcatattct accccgctgt
     2281 ctgtagtaaa cagatataca ctccccgaac tgattgccgg tggaacaatt ttataggggt
     2341 cgctgcctgt tacgctcaca aaaaagtgct aatcattaaa aataatactt ttggacttac
     2401 gtctgcaaaa ataggataat ggcaaaattc ggcaaatggt taggaggagg actcggatgg
     2461 gcactaggtg gacccattgg agctgtgttt ggctttgctg taggaagcat ggttgatgcc
     2521 accagttttg aaaaaaacga ggagatagaa ggacaattcc gccaaacgag agaacgctac
     2581 cgccaccata ccactcccgg tgattttacc gcatccttac tgatactcac agcagtagtg
     2641 atgaaagccg atggaaaaat catgaaatct gaattggatt atgtgcgcaa tttctttctc
     2701 cggcagtttg gggagtaccg tacgcagcag atcctgaaag agctacagca gattctgaaa
     2761 aaggaaattc ccgtgcagga ggtttgcatg gaaatcaggc attatatgga gcaccccatg
     2821 cgccttcagc ttattcatta cctttttgga atagcagccg cagatggaag cgtggatagt
     2881 gccgagcaaa gggttatcct tcagatagcg ggctatcttg gtgttaattc acgggatttt
     2941 gagtcgattc aggctatgtt tgtccgcgac acagtaagcg cttataaaat ccttgaaatt
     3001 gaatcttcag cgagtgatga agaggtaaaa aaagcgtacc gaaaaatggc agtgcgctac
     3061 catcctgata aagtcgccag tcttggtgaa gaacatcaaa aagctgcaca ggaaaaattt
     3121 caaaaagtgc aggaggccta cgaaaacatt cgaaaagaaa ggggaatgaa ttaagtttct
     3181 actattcgac ctcagctcta tatcctcttc cttttctcgt caaactattg gttttcaaat
     3241 atcttggaac taattattca taatccgacc cttacatcgc gcagacagag atggttcggg
     3301 aattttgcca aattactttt taccaacaaa atcaggattc ctgttaataa ccagcctgca
     3361 atcagccctt actgctctat ttttaagaat agaaactaaa gcttatggaa tccagaaaat
     3421 tcagtgaaga gacccttact gcggtcgtga agaaaaaaaa catcttgttg gtgattgaat
     3481 attgcctcga aaacaagatt gaattcgtgg tgactcccgg agcgggaagt gatgatttcc
     3541 tgattggatt tgtgatcaag gaacttggtg ctgctgttgc gttgggaatg tcgctcaaag
     3601 atcttaaaat agatttacag ggattacccg gatttatttc ggtaaatcaa atccgcagta
     3661 aaaaaggcaa tgaagcaagt ccggcaaaag gttcgaaaaa cgaatcacgc gacatgagcg
     3721 acgaacttga aattcctaca atggaaaatg cattaaaatt cgacctcgaa aacgcataat
     3781 cgtttttcta tctgataaaa ggaaatcccc atcggcttcg gtggggattt ttttatttaa
     3841 cgaagacgaa gactaaaact caatcacatt tccaccttca cttttaacta ataactttta
     3901 actaataact ttcaccaaaa acttttcacc ttaagatatt ctgctcca
//
LOCUS       DAOMYG010000304         3925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8216_length_3925_cov_7.514987, whole genome shotgun sequence.
ACCESSION   DAOMYG010000304 DAOMYG010000000
VERSION     DAOMYG010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8216_length_3925_cov_7.514987"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1709
                     /locus_tag="PK610_09910"
     CDS             90..1709
                     /locus_tag="PK610_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ36292.1"
                     /translation="MISTSNLTLQFGKRILFDEVDVQFTEGNCYGVIGANGAGKSTFL
                     KILSGEVDPTKGQVNILPGKRMAVLKQDHFEFDECRVLDTVIMGHKKLWAISMEKESI
                     YAKPEMTEADGNRAAELESEFAEMDGWNAETDAAEMLSNLGVKEEYHHQQMKDIAGNL
                     KVRILLAQALFGNPDILILDEPTNDLDVETISWLEDFLADFRNTVIVVSHDRHFLDTV
                     CTHIADIDFGKITLYTGNYTFWYQASQLALKQRTEQNKKVEDKRKELQEFVARFSANA
                     AKSKQATSRKKLLEKLQIDDIRPSSRKYPAIILNQERDAGDQILRVEHLTISNEEGVL
                     VNDISFKVDKGDKIAILSKNSTAITALLEVLAGEREPQSGSLEWGITIKRAYLPNENS
                     HYFQEDLSLVDWLRQYSVNKDEDYIRGFLGKMLFSGQEALKSVKVLSGGEKVRCMTSR
                     LMLMQGNFLLLDEPTNHLDLESITAFNNALIDFKGMVMFTSHDHEFVQTAANRIIEIT
                     PGGIIDKKLTYDEYLADPKVKELRSAEGILV"
     gene            complement(1710..2096)
                     /locus_tag="PK610_09915"
     CDS             complement(1710..2096)
                     /locus_tag="PK610_09915"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36293.1"
                     /translation="MRIVFTLFLSFVFLSSSAQVRKVVIKTFPSGQPEVIYHLKGKEM
                     NAEKIKEEVYYENGKLEYTGEFKNGTEHGEWVYYYPSGNIKAKEYWKNGVEHGTWKEY
                     HPDGQLAREIVYKDGKIVDTVVKKRQ"
     gene            complement(2118..>3925)
                     /gene="dnaE"
                     /locus_tag="PK610_09920"
     CDS             complement(2118..>3925)
                     /gene="dnaE"
                     /locus_tag="PK610_09920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRJ36294.1"
                     /translation="LTIIKDACRIVKERHGISLDPDEFPLDDAKTYELYQRGETNGTF
                     QFESPGMQKYLRELKPDTFADLIAMNALYRPGPLEYIPNFIRRKHGIEPISYDLPEME
                     EYLKETYGITVYQEQVMLLSQKLADFSKGKADELRKAMGKKIRAKLDELKPLFVEKAK
                     EKGHPEDKLNKIWTDWEAFASYAFNKSHSTCYAYVAYQTAYLKAHYPAEYMASVLTHN
                     MSDIKKVTFFMEECKRMKIPVLGPSVNESELKFTVNTQGAVRFGLGAIKGVGENAVEA
                     IVRERKENGKYLSIFDFTRRIDLRSANKKTLENLALAGAFDDFGIYRSVYFHDDGDNN
                     TFLSKAIRFGTSLQEGAQSSQVSLFGEESAEMTPEPNIPKSEPWPTLYQLNREKEVVG
                     IYISGHPLDDFRYDLDNLCTPGAHCGMLGILGKDQLHRELRIAGIVTKIEHRQTKTGK
                     PFGSFEMEDYEGTARLMLFGDDYMKFRQYLVENTFIFIRGKVQNRRFGNEIKEDEFEF
                     KVGMIDLLADARERYVKGINLSINPYRVDERFVDELDGLLQRYKGKTRYRVTLLDSLE
                     KIRIDMPSKNVGVEISNEFLDELKSLEHLEFEFLL"
BASE COUNT         1115 a          865 c          802 g         1143 t
ORIGIN      
        1 gtcaaagctg agaaatcgtg aacggcgccg ataagcacta cgccgaatgc gatcctacct
       61 ttgcggccag ttttacaata tttgatgtta tgatcagtac ctccaattta accctgcagt
      121 tcggaaaacg aattttattt gatgaagttg atgttcagtt taccgaaggg aactgttatg
      181 gtgttatcgg agccaacgga gccggaaaat caactttcct gaaaatactc tccggtgagg
      241 tcgacccaac caaaggacaa gttaatatcc ttccaggaaa acgaatggct gtactgaaac
      301 aggaccactt cgaatttgat gaatgcagag tgctggatac agtcataatg ggacataaaa
      361 aattatgggc catttcgatg gaaaaagaaa gcatttatgc aaaacctgaa atgacagaag
      421 cagatggcaa ccgcgccgct gaactggaat cggagtttgc agaaatggat ggttggaatg
      481 ccgaaaccga tgctgccgaa atgctgtcaa atctcggcgt aaaagaagaa taccaccacc
      541 agcaaatgaa agatattgca ggtaacttaa aggttcgaat tctgcttgca caggctttat
      601 tcggaaatcc ggatattctg atcctcgatg agccaaccaa cgaccttgat gttgaaacca
      661 tttcctggct cgaagacttt ctggcagatt tcagaaatac agtgatcgtt gtttcgcacg
      721 accgtcactt tctcgacacg gtttgtaccc atatcgcaga tatcgacttt ggaaaaatta
      781 ctctttatac cggtaactac acattttggt atcaggcttc tcagcttgca ctcaagcagc
      841 gaacagaaca aaacaagaaa gtagaagata aaagaaagga attgcaggaa tttgttgcgc
      901 gatttagtgc caacgcagca aaaagcaaac aggcaaccag caggaaaaaa ttactcgaga
      961 aactgcaaat cgatgatatc cgaccttctt caagaaagta tcctgccatc attcttaacc
     1021 aggaacgtga tgccggtgat cagattctaa gagtagaaca cctcaccatt agtaacgaag
     1081 aaggcgttct cgtaaatgat atcagtttta aagtggataa aggagataaa atcgccattc
     1141 tctcgaagaa ctcaacagca attactgccc tgctcgaagt acttgccggc gaacgcgaac
     1201 ctcaatctgg tagtttggaa tggggtatta ccattaaacg cgcatatctt cctaatgaaa
     1261 actcacacta ttttcaggag gacctttctt tggtcgactg gctgcgtcag tattccgtaa
     1321 ataaggatga agattatatc cgcggtttcc ttggaaaaat gttgttttca ggacaagaag
     1381 cacttaagtc agtgaaagta ttgtcgggag gagaaaaagt gcgttgtatg acttcacgtt
     1441 tgatgctgat gcaaggtaat ttccttttgc ttgatgaacc aaccaaccac ctcgatctcg
     1501 aatccattac agcatttaac aatgcattga ttgattttaa aggaatggtg atgtttactt
     1561 cgcatgacca tgagtttgtt cagactgcag caaacaggat catcgaaatt actccgggag
     1621 gaattattga taagaaactg acttacgatg aatacctggc agatcctaaa gtaaaagaac
     1681 ttcgctccgc agagggaata ttggtgtaat tactgtcgtt tttttacgac ggtatcaacg
     1741 atttttccat ccttatacac gatctcacgg gcgagttgac catcgggatg gtattctttc
     1801 catgtgccgt gttcaacacc gtttttccag tattccttcg cttttatatt acctgatgga
     1861 tagtagtaaa cccattctcc atgttctgtt ccgtttttaa attctccggt atattccagt
     1921 ttgccgtttt cataatagac ttcttcctta attttttcgg cattcatctc tttgcctttc
     1981 aaatggtaaa tcacttccgg ttgaccactc gggaaagttt tgatgacaac ctttcttacc
     2041 tgggctgacg atgatagaaa tacaaacgaa agaaataatg tgaatacaat acgcatagcg
     2101 gcattcggtt taattagtta aagcaaaaac tcgaattcaa gatgttcgag cgatttcaat
     2161 tcgtcaagaa actcattgct gatttcaaca ccaacatttt tactgggcat atcaatcctt
     2221 attttttcaa gactgtccag cagcgtaacc cggtatctgg ttttcccctt ataacgttgt
     2281 aaaagaccat ccaactcatc aacaaatcgt tcgtccacac ggtacggatt aatgctcagg
     2341 ttgatgcctt ttacgtatct ctccctggcg tcggcaagca aatcgatcat tcctacttta
     2401 aactcaaact catcttcttt gatctcgttt ccgaatcgtc ggttctgtac ttttccacga
     2461 atgaaaatga atgtgttctc tacaaggtat tgtctgaatt tcatgtagtc atcaccaaat
     2521 aacatgaggc gtgcagtgcc ttcataatct tccatttcga aagatccgaa tggttttcct
     2581 gttttggtct gacggtgttc aatcttagtt acaattccgg caatccttaa ttcgcggtgc
     2641 aattgatctt tgcctaaaat tccaagcatt ccgcaatgag cgcctggcgt gcagagatta
     2701 tcgagatcgt acctgaaatc atccagcgga tgccctgaaa tgtaaattcc taccacttct
     2761 ttttccctgt taagctggta caaagtgggc catggttcgc ttttgggaat gttgggctca
     2821 ggagtcattt ctgcgctctc ttcaccaaac aaactaactt gcgatgattg cgctccttcc
     2881 tgcagcgaag ttccaaaacg gatcgctttc gacaagaagg tattgttatc gccatcgtcg
     2941 tggaaataca cagaacggta aataccaaaa tcatcaaatg ctcctgccaa agcaagattt
     3001 tcgagcgttt ttttattggc agaacgaaga tctattctcc gggtaaaatc aaaaatagag
     3061 agatattttc cgttttcttt tcgctcccga acaatggctt caaccgcatt ttcaccaacg
     3121 cctttaattg ctcccaaccc aaaacgcacc gcaccttgag tattaactgt aaacttaagt
     3181 tccgactcat ttacacttgg tcccagtaca ggaattttca ttcgcttaca ctcttccata
     3241 aagaaagtga ccttcttaat atcactcatg ttatgggtca gcaccgatgc catatactcg
     3301 gcaggatagt gagcctttaa ataagcagtt tggtatgcga cataggcata acaggtggag
     3361 tgcgatttgt tgaaagcata tgatgcaaac gcttcccagt cggtccagat cttattgagt
     3421 ttatcttcag gatgaccttt ttctttggcc ttttctacaa acaatggttt tagctcatcc
     3481 agtttggcgc gaatcttttt ccccatcgcc ttgcgcaact catccgcttt tcccttagaa
     3541 aaatccgcaa gtttttgaga aagcaacatc acctgttctt ggtaaaccgt aattccgtag
     3601 gtctccttga ggtattcttc catttccgga agatcgtagg agatgggttc tattccgtgc
     3661 ttacgtcgga taaaattcgg tatatattcc aacggaccgg gtcggtaaag cgcattcatg
     3721 gctatgagat ccgcaaacgt atctggcttg agttcgcgca ggtatttctg cattcccgga
     3781 ctttcaaact ggaaggttcc gttggtttct ccacgctgat aaagctcata ggttttggca
     3841 tcatccaatg ggaactcatc aggatcgagt gatattccgt gacgctcttt cacaatgcgg
     3901 caggcatctt ttatgatggt caggg
//
LOCUS       DAOMYG010000305         3906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8272_length_3906_cov_7.741106, whole genome shotgun sequence.
ACCESSION   DAOMYG010000305 DAOMYG010000000
VERSION     DAOMYG010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8272_length_3906_cov_7.741106"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..880
                     /locus_tag="PK610_09925"
     CDS             104..880
                     /locus_tag="PK610_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRJ36295.1"
                     /translation="MEFIKIEMVGQVAKVLLNRPDKFNSFNRQMSLELQQALDQCKND
                     DNVRCILLTSSGKAFCAGQDLSEAIDPSGPGIEKIVFEHYNPMIERIRNIEKPVVCAV
                     NGVAAGAGASLAISCDIVLASASASFTQAFSKIGLIPDSGGTHILPRIVGYHRAAALM
                     FLSEKISAEEGKELGFVWKVFPDENFADDAMKLAQQLAAMPTRGIGLTKRLLNEGISN
                     DLSTQLVREGKVQVEAANTSDHKEGVKAFLEKRAPVFTGK"
     gene            896..2071
                     /locus_tag="PK610_09930"
     CDS             896..2071
                     /locus_tag="PK610_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRJ36296.1"
                     /translation="MKIGIAGAGAMGSGIAQVAAMNGHEVVLYDAFAPSIEKADTSLN
                     KILNSLQEKGKISNAAEILNRIKITGNINDFSGCGLVIEAIIEQLPAKQELFRKLEEI
                     TGENSILASNTSSLSVTLIAASCKKPERVVGIHFFNPAPLMPLVEIIPAVQSDLTILE
                     QARKLIDSWGKTTVLAKDTPGFIVNRVARPYYGEAIRIYEEGVADFATIDWAMKEMGG
                     FRMGPFELMDFIGNDINYTVTETVFREFFYDPRYRPSFTQKRQMESGRLGRKSGRGYY
                     DYSEGAQLPEAKKDKVLGEKIVNRVLAMLINEAADALYLNIATAKDLDLAMTKGVNYP
                     KGLLTWCDELGAEKVYAQLRELQDIYGEDRYRPSPLLRKHAENKTRFFSSSIVEKGV"
     gene            2125..2748
                     /gene="recR"
                     /locus_tag="PK610_09935"
     CDS             2125..2748
                     /gene="recR"
                     /locus_tag="PK610_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRJ36297.1"
                     /translation="MQYSSKVIEEAVEQLASLPSIGRRSALRLVLSMLKRRPEEMERF
                     AQAFLDLNSKIKYCKECHNISDEDTCSICNNPRRDKSLVCVVEDIRDVMAIENTRQFN
                     GVYHVLGGIISPMEGIGPSDITIDSLVEKVNKGTVKEIILALSTTMEGDTTGFFIFRK
                     LKEKTISISNIARGISVGDELEYADEVTLGRSILNRVPYDNSYGVAK"
     gene            2807..>3906
                     /locus_tag="PK610_09940"
     CDS             2807..>3906
                     /locus_tag="PK610_09940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ36298.1"
                     /translation="MKLSVIIVNYNVEYFLEQCLNSVVAASKGIAVEVFVVDNNSVDG
                     SCAMVKRRFPEVQLIENHFNAGFSKANNQAMMLAKGEYILLLNPDTVVEEDTFRKVIR
                     FMDEHPDAGGLGVKMIDGKGKFLPESKRGLPTPSVAFYKIFGISRLFPRSKRFGRYHL
                     GYLDKDQSHEIEILSGAFMLMRKTALDKVGLLDEAFFMYGEDIDLSYRITIGGYKNYY
                     FPDTTIIHYKGESTKKSSVNYVFVFYNAMIIFARKHFSEKNARTFSLLIHVAIYLRAF
                     VAILNRFVKRIILPAIDFTCIVAGSWFIASHYETYRSIDFPDEVLNYALPAYTLTWLT
                     GVWLSGGYDYPIRLWKIVRGLIYGTAFILMVYG"
BASE COUNT         1113 a          821 c          898 g         1074 t
ORIGIN      
        1 actctccgat atggaagcca ttgagattgg tccgagggct cggttaatgg aatgatggta
       61 tttaaagcac agccattata tttgtccaac tcaatcatca attatggaat tcataaaaat
      121 tgaaatggtc ggccaggtcg cgaaagtctt gcttaaccgt ccggataaat tcaacagttt
      181 caaccgacag atgtcgctcg aactgcagca agcgctcgac caatgcaaaa acgacgataa
      241 tgttcgttgc atcttgctca cttcttctgg taaagcattt tgtgctggtc aggatttaag
      301 cgaagccatc gatccaagcg gaccgggtat tgagaaaatt gtatttgaac attacaatcc
      361 gatgattgag cgcatccgca acattgaaaa accggttgta tgtgctgtaa acggagtagc
      421 tgccggtgcc ggtgcttctt tggctatttc ctgtgatata gtacttgcaa gcgcatctgc
      481 atcctttacc caggcattta gcaaaatcgg attaattccg gatagtggtg gaactcatat
      541 tttacctcgt attgtggggt atcacagagc tgcggcatta atgtttttaa gcgaaaaaat
      601 cagtgcagaa gaaggtaaag aacttggatt tgtctggaag gtttttcccg atgaaaattt
      661 tgccgatgat gcaatgaaat tggctcagca acttgcagcg atgcctacac gcggaattgg
      721 tttaaccaag cgtttactca acgaaggaat cagcaatgac ctgagcactc aactcgtacg
      781 cgaaggaaag gttcaggttg aggcagcaaa tacctctgat cacaaagagg gtgtaaaagc
      841 gttccttgaa aagcgtgcac cggtatttac aggaaaataa cagcatatga gcactatgaa
      901 aatcggaata gcaggtgcag gggcaatggg aagtggaatt gcacaggttg cagccatgaa
      961 cggacacgag gttgtacttt atgatgcttt tgctccttca atcgaaaaag cagacacatc
     1021 gctgaataaa atactgaaca gtctgcagga aaaaggtaag atcagcaatg cggcagaaat
     1081 tcttaaccga ataaaaatta cagggaacat caatgacttt tccgggtgtg gtttggtcat
     1141 tgaagccatt attgaacagc ttcctgcaaa gcaggaattg tttcgtaaac tcgaagaaat
     1201 tacaggcgaa aacagcattt tggcaagcaa tacttcgtca ttgtccgtga cactgatcgc
     1261 cgcctcctgc aagaagcctg aacgtgtggt tggaattcac tttttcaatc ctgccccact
     1321 catgcccctg gtggaaatta ttccagctgt acaatctgat cttaccatcc ttgaacaagc
     1381 gcggaaactg attgattcat ggggaaagac aactgtcctg gctaaagaca ctcccggttt
     1441 tattgtaaat cgtgttgcaa gaccatacta tggtgaggcc atccgaatct atgaagaagg
     1501 tgttgcagac tttgccacca tagactgggc tatgaaggaa atgggtggtt tcagaatggg
     1561 accgttcgaa ctgatggatt tcattgggaa cgatatcaat tataccgtta ccgaaactgt
     1621 atttcgcgaa ttcttttacg atccccgcta ccgcccttcg tttacccaaa aacggcaaat
     1681 ggagtccgga cgacttggac gtaaatcggg acgaggatat tacgattatt cggaaggcgc
     1741 tcaattaccc gaagcgaaaa aggataaagt tttgggagag aaaattgtga accgcgtact
     1801 tgccatgctt atcaatgaag ctgcagatgc tttatatctc aacattgcca cagcaaaaga
     1861 cctggatctg gccatgacca agggtgtaaa ctacccgaaa ggcctgctga cctggtgcga
     1921 tgagttggga gcagaaaagg tctatgcaca gctaagggaa cttcaggata tctacggaga
     1981 agaccgttac cgtccgagtc cattgttgcg gaaacatgcg gagaacaaaa cccggttttt
     2041 ctcatcatca attgttgaaa aaggagtgta aatgtaaact cctgcccacc cttggtaatg
     2101 ctctcttctt gctatttttg agggatgcaa tactcctcca aagtcattga agaagcagtt
     2161 gaacaattag cttcccttcc cagtataggt cgtcgctcgg cattacggct tgtattaagc
     2221 atgctgaaac gccgtcctga agaaatggag cgatttgccc aggctttttt ggatctgaac
     2281 agcaaaatca agtattgtaa ggaatgtcat aatatttcag atgaagacac ttgttccatt
     2341 tgcaataatc cgcgcagaga caagagtctg gtttgtgtgg tagaagatat ccgcgatgtt
     2401 atggccattg aaaacacacg ccagtttaac ggtgtttatc atgtattggg aggcatcatt
     2461 tctccaatgg aaggcattgg tccctccgat atcaccatcg attcgcttgt ggaaaaggtt
     2521 aacaaaggca ctgtaaagga aattattctg gcgttaagca caacgatgga aggtgataca
     2581 acaggtttct ttattttccg caaactcaag gaaaaaacga tttccatcag caatatagcc
     2641 agaggtattt cggttggaga tgagctggag tacgccgatg aggtaacatt gggtcgttct
     2701 atccttaacc gcgtgccata cgacaattcc tacggggtgg caaaataatc ccgctttggt
     2761 tacgtttgtt tctcgctgat gattttccct tattttgcag ttccccatga agctctccgt
     2821 aatcatcgtt aattacaatg tcgagtattt tctcgaacaa tgcctcaatt cggtagttgc
     2881 tgcttctaag ggtattgccg ttgaggtttt tgtggtagat aataattcag tcgacggttc
     2941 atgcgctatg gtgaaacgca ggttcccgga agtacagctt atcgaaaatc attttaacgc
     3001 cggattctcc aaagccaata accaggcaat gatgctggcc aaaggcgaat acatcctcct
     3061 gctcaatccg gatactgttg tagaagaaga caccttccgc aaagtcattc gtttcatgga
     3121 tgaacatcct gatgccggcg gactcggtgt aaaaatgatt gatggaaaag ggaaatttct
     3181 tcccgaatcc aagcgagggc tgcctacgcc atccgttgca ttctataaaa tattcggcat
     3241 ctcccgatta ttcccaagat caaaacggtt tggacgctat cacttgggct atctcgacaa
     3301 ggatcagtcc cacgaaatag aaattttatc cggtgccttt atgctgatgc gcaaaacggc
     3361 gttggataaa gtcggactac tcgatgaagc atttttcatg tacggagagg atattgatct
     3421 ttcgtacaga ataacaatag gaggatataa aaattactat ttccccgata ccaccatcat
     3481 tcattacaag ggagaaagca ctaaaaaaag cagcgtcaat tatgtatttg tgttttacaa
     3541 tgcgatgatc atttttgcgc gtaaacattt ttccgagaag aatgcacgca ctttttcatt
     3601 gttgattcac gttgccattt atctccgcgc atttgttgcc atccttaacc ggtttgtaaa
     3661 acgaattatt cttccggcca ttgattttac ctgtattgtt gcaggatcct ggtttatagc
     3721 ctcgcattat gaaacctacc ggtctatcga ttttccggat gaagtactga actatgcctt
     3781 acctgcctat acgctaacct ggttaaccgg tgtttggttg tcgggcggat acgattaccc
     3841 cattcgccta tggaaaattg tgcgcggact gatctatgga acggcattca ttcttatggt
     3901 ttatgg
//
LOCUS       DAOMYG010000306         3884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8334_length_3884_cov_8.194307, whole genome shotgun sequence.
ACCESSION   DAOMYG010000306 DAOMYG010000000
VERSION     DAOMYG010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8334_length_3884_cov_8.194307"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="PK610_09945"
     CDS             <1..1330
                     /locus_tag="PK610_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36299.1"
                     /translation="EKFASYDPHKMGVIFKLHANKNTGATNLFNFSKVCETIYQNVYA
                     DYDKGKKSVAKFVKKIGLKPEDSELDKIHKVENYVKANTLILDIDIPAWGDVKSILSS
                     SITLDNKKKKMIASNVGIMRVFAYVYTFLGIDHQVVMTCDRYDKFFDREFESYHYLDE
                     YLIYFPNQDMLISPCENYSRSKFISQGCYNTYALFIVPVTVGTMKSAISKVKFIEALP
                     ETANTDSLYLNCSLNTSNSKLAINYRRTALGYYAIQYQPFYDLLDKETLTEFEKVFTA
                     FLTSNKEDIKKFKIDNVGVDNVGKPLITEAELECEKFVEMAGDKYVVKLGELIGPQAE
                     LYQEGERKLPVENGFNRLYYRRIEFVVPDGYKIADASKLKMDFKHTNDKGKVTMEFKS
                     EYKVQGNKLIVDCTEYYRQISYPVELFEEFRKVINAAADFNKIAIVLEKS"
     gene            1330..1869
                     /locus_tag="PK610_09950"
     CDS             1330..1869
                     /locus_tag="PK610_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRJ36300.1"
                     /translation="MGRKLNMDELGRLSPEEFKQREKTPVIVILDNIRSHHNVGSVFR
                     TSDAFAIQAVWLCGFTPIPPHREIHKTALGATESVEWKHFETTIDAIEAARQLNYKVI
                     AVEQAENAIPLHKVTTEYNGNLAFVFGNEVGGVEQEAIDKCNAVVEIPQWGTKHSLNI
                     SVCAGLVLWEVFRHRLDLH"
     gene            2003..2356
                     /locus_tag="PK610_09955"
     CDS             2003..2356
                     /locus_tag="PK610_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36301.1"
                     /translation="MKNSILTFFIATVITVASLFVFGGLIAKPVSGDKVCPSCMYKNF
                     KLWGKVKVVESFPDIKVQIVESFPDLKVKQVTSFPDACGKWQFVESFPDFKIQFVTSF
                     PDIKIKYVESFPGCN"
     gene            complement(2359..3225)
                     /locus_tag="PK610_09960"
     CDS             complement(2359..3225)
                     /locus_tag="PK610_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36302.1"
                     /translation="MIRWYCLFLLLISCQHESRISESLKQEILAINTWIHIPSPYMEA
                     SSLYNESNTEICGLEAYKPPRSGKRKKTYRITGKDTIVLQDFDYKAVTELHLSGNLNF
                     HSNTLALFSEIETLSISTFAGITESKGLKFKKLKVIKMYHTQLHPAFAEIAGDQLCEF
                     HASFSDLDSFPINKLRSLRKISLVHSRLPEITMFNQLENLQDLTIAKCWSKSDMSKLD
                     LSKTPCLRSLLIIYTSNLKKIPSNLLHSNLEVCSLLRGDFSLEEKNQIYAFGERIRKK
                     NGINQMKKPTYE"
     gene            complement(3262..>3884)
                     /locus_tag="PK610_09965"
     CDS             complement(3262..>3884)
                     /locus_tag="PK610_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRJ36303.1"
                     /translation="SAKGLEFPYVYIVGMEENLFPSQLALNSREELEEERRLFYVAIT
                     RAEKRCTLSYANTRYRWGNLTHCEPSRFIDEVDSKYLEMPPAPKELNFDEEWDEPSFR
                     KMKVPKKDSLFEKEPSKKAAVPATPSPSKRNLVKLDEKRPATSVPSSDSDSDLKVGQN
                     VHHEKFGKGKVIQIEGIEPNRKATVFFPAVGQKQLLLRFAKLDILD"
BASE COUNT         1156 a          686 c          832 g         1210 t
ORIGIN      
        1 tgaaaaattt gcatcctacg accctcataa aatgggagtg atatttaagt tgcatgcaaa
       61 taaaaacaca ggtgcaacaa acctttttaa cttttcgaaa gtttgtgaaa cgatctacca
      121 gaatgtttat gccgactacg acaaagggaa gaaatcagtt gcgaaattcg tgaaaaagat
      181 tggcctgaaa ccagaagatt cagaacttga taagatccat aaggttgaaa attacgtaaa
      241 agcaaataca ctcattctgg atattgatat tcctgcatgg ggcgatgtaa aatccatcct
      301 aagcagttca attactcttg ataacaagaa gaagaagatg attgcttcga atgtgggaat
      361 catgcgggta tttgcatacg tctatacatt tcttggtatc gatcatcaag tggtaatgac
      421 ctgcgataga tacgataagt tttttgaccg tgagtttgag tcctaccatt atttggatga
      481 gtatttgatt tatttcccta atcaggatat gttgatttca ccttgtgaaa attattcgcg
      541 ttcaaagttt atttctcagg gttgctataa tacctatgca ttgtttattg ttccggtaac
      601 tgttggaaca atgaaaagtg caataagtaa agtgaaattc attgaagcac ttcctgaaac
      661 agcaaataca gacagtcttt atttgaactg ttcattgaat acttcaaata gcaagcttgc
      721 aattaattac cgcagaaccg cacttggcta ttacgctata cagtaccagc ccttttatga
      781 tttactggat aaagaaacgc taactgaatt cgaaaaggtg tttaccgcat tcctgacgag
      841 taacaaggag gatataaaaa aattcaaaat cgataatgta ggtgttgata atgttggtaa
      901 accactgatt acggaagctg agctggaatg cgagaaattt gtagagatgg caggagataa
      961 gtatgttgtt aaacttggtg agttaatagg tccacaagca gagctgtatc aggaaggaga
     1021 gcgcaaattg cctgtagaga atgggtttaa tcgtttgtat taccgtcgaa tcgaatttgt
     1081 tgttccggat gggtataaga tcgctgacgc ttccaaattg aagatggatt ttaaacatac
     1141 caacgataag ggaaaagtta cgatggagtt taaatcagaa tacaaagtgc agggcaataa
     1201 actaattgtg gattgcacag agtactatcg tcagatcagc tatcctgttg agttatttga
     1261 ggagttcaga aaggtgatca atgctgcagc agattttaat aaaatcgcaa ttgtacttga
     1321 gaaatcctga tgggacgaaa actgaatatg gacgagttgg gacgattgtc gcctgaggag
     1381 tttaagcaga gagagaaaac gccggtaatc gtaattcttg ataatatccg tagtcatcat
     1441 aacgtgggaa gtgtatttcg cacatcagat gcttttgcaa tacaggctgt ttggctttgc
     1501 ggatttacac ctattccacc acatagggag attcataaaa ctgcacttgg tgcaactgag
     1561 tccgtagagt ggaagcattt cgaaactaca atagatgcca ttgaagcagc gaggcaactc
     1621 aactataaag ttattgctgt tgagcaggca gaaaatgcaa tccctcttca taaagtaaca
     1681 acagaatata acggaaacct ggcatttgtt ttcggaaatg aggttggggg agttgaacaa
     1741 gaagccatcg ataaatgcaa tgcggttgtg gagattccac aatggggaac caagcattca
     1801 cttaacattt ctgtttgtgc aggccttgtt ctttgggagg tttttcgcca ccgtcttgac
     1861 ttgcattaga atttcagtta ccttatgtcc atgtttaagt gcattacact gggtttttgt
     1921 tactggaatt cttaccttca ggtttgtatt tggttcggtt cccggaaagt aatattagtc
     1981 cgaaacctta aatgattgta aaatgaaaaa cagtattcta acattcttta ttgcaacagt
     2041 aattactgtc gcttccttat ttgtatttgg aggcttaatc gcaaaacccg tcagcggaga
     2101 caaggtttgt ccctcttgta tgtacaaaaa ctttaaactc tggggcaagg tgaaagtggt
     2161 agagtcattt cctgatatta aagtacagat cgtagagtct tttccggatc ttaaagtaaa
     2221 gcaagtcact tctttcccgg atgcttgtgg taaatggcaa tttgtagaat ccttcccaga
     2281 ttttaaaatt cagtttgtga cctccttccc tgatatcaaa atcaaatacg tggagtcttt
     2341 ccctggatgt aattgatttt attcgtaagt cggttttttc atttggttga ttccattctt
     2401 ttttcgaatt cgttctccaa atgcgtaaat ctgatttttt tcttccaggg agaaatcacc
     2461 tctcaaaagg ctgcatactt caaggtttga atggagtaga ttacttggta tctttttaag
     2521 attactggta taaataatca ataaacttct cagacaaggt gttttgctca aatccagttt
     2581 gctcatatcg cttttcgacc agcatttagc aatagtaaga tcctgaagat tctccagttg
     2641 attaaacatt gtgatttccg gtagccttga gtgtacgagg cttatttttc gcaatgagcg
     2701 aagtttgttt atgggaaaac tatcgagatc tgaaaatgat gcatggaatt cgcatagctg
     2761 gtcacctgca atttccgcaa aggcgggatg aagttgagta tggtacattt taatcacttt
     2821 cagttttttg aatttgaggc cttttgattc agtaattccg gcaaaggtgg agatagaaag
     2881 ggtttcaatc tctgaaaata acgcaagggt attcgaatga aaatttagat tcccgcttaa
     2941 atgaagttct gtaaccgctt tataatcgaa atcctgaagt acaatagtgt ccttgccagt
     3001 tatcctatag gttttttttc gttttcctga tcgcggaggc ttgtaagctt ccaacccaca
     3061 gatttcggtg tttgattcat tatacaagga gcttgcttcc atatatggcg atggaatatg
     3121 tatccaggtg tttatggcca ggatctcctg tttaaggctc tctgaaattc gcgattcgtg
     3181 ctgacacgaa atcaatagta ggaataagca ataccatctg atcatgatgg ttaaatacta
     3241 caacagaaaa aagtaacaga atcagtctaa aatatcaagt ttggcaaacc ttaacaatag
     3301 ttgcttttgc ccaacagcag gaaaaaacac ggttgctttt ctattgggct caatgccttc
     3361 aatttgtatg actttcccct ttccaaattt ttcatgatgc acgttttgtc caactttcaa
     3421 atccgagtca gaatcagatg aaggaacgga ggttgcaggt ctcttttcgt cgagttttac
     3481 caggttccgt tttgagggag agggagtagc tggaacagct gctttttttg aaggttcttt
     3541 ctcaaacaaa ctgtctttct tagggacttt catttttcgg aatgacggct cgtcccattc
     3601 ttcatcaaag ttgagctctt ttggagcagg aggcatttcc aggtatttgg aatcaacctc
     3661 atcaatgaac cgactaggtt cgcaatgggt aaggtttccc caacggtatc gggtattggc
     3721 atagctgagt gtgcatcttt tttcagcgcg ggtgatggca acataaaaca aacgacgctc
     3781 ttcttcgagt tcttcgcggg aattcaaagc cagttgcgaa ggaaatagat tttcttccat
     3841 gcctacaatg tacacatacg gaaactccag accttttgct gagt
//
LOCUS       DAOMYG010000307         3879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8358_length_3879_cov_9.079236, whole genome shotgun sequence.
ACCESSION   DAOMYG010000307 DAOMYG010000000
VERSION     DAOMYG010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8358_length_3879_cov_9.079236"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2339
                     /locus_tag="PK610_09970"
     CDS             <1..2339
                     /locus_tag="PK610_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRJ36304.1"
                     /translation="SPPKEILDLVDIQPTPMVRVSSKGSYMVLLERYVFKTLEELSEE
                     ELRLAGLRINPRNFGPSRANYAHSLRIMNMNTGKAITVNGAPSNARIGSLSISRDEKY
                     AAFTNTTAERIELWIMELVSGKCTLLTSGLAVNAVLGSPYSWSSDNKGLYCKLVTDQV
                     PLSSIYRKPLPEGPVVQVSGGKKAPVRTFQDLLRNKNDEKLFESFAASELVYFGVDGS
                     RRKFASRGMYYSADESPDGKFVLLTSIQYPFSYIVQLDRFPVSYAVHEASGKFLNEFY
                     RKPLIEELPNGFDAVETGKRDIEWRNDQNATLVWVEAQDGGDPAKEVAIRDHVYQVEA
                     PFTGAPQLLATTELRFSGLSFGNASMMLVRENWWKSRRTKTTMVDPTSKSKPKVIFDR
                     SEEDVYNDPGNFLMDYNENGHYVLRTSKDKKKLYLSGQGYSPAGNRPFLDEYTIADGK
                     RKRLWQADGKTTYENIATVFDPEKQIMLTTVESPEMNPNFYLRSGKSAKKITNFPNPY
                     ASFMGVTKQRIFYKRKDGVDLSATLYLPKGYDKVKDGPLPVVMWAYPREFKDKAQAGQ
                     VKESPHRFAQINYGSPVYWAMRGYAILDNTDFPIVGEGKTEPNDSFIEQLVANAEAAI
                     NTLAEMGVGDRKRVAIGGHSYGAFMTANLMAHCDLFAAGIARSGAYNRTLTPFGFQAE
                     ERTFWEAKKVYMEMSPFYFAEKINEPLLLIHGDADNNPGTFTLQSERLFAAIKGLGGT
                     ARLVLLPYESHGYSARENILHMLWEQDQWLEAYVKNKK"
     gene            complement(2453..3454)
                     /locus_tag="PK610_09975"
     CDS             complement(2453..3454)
                     /locus_tag="PK610_09975"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRJ36305.1"
                     /translation="MNAKQVKIGVIGGGSWGTAIVKLLCNQVDTVYWWVHKKESVDHI
                     RNFHHNPRYLQSVEFNPGQLVVTNRLEDIIPKCDILIMATPAAFLHSVFQKYPAENFK
                     GKIIFSAIKGMVPEYNAIPATYFKEQLQVPYEKIGIICGPCHAEEVALERLSYLTIGC
                     SDTSVAQHMADLLACRYIRTTVSDDLFGTELSAVLKNIYAIASGICGGLGFGDNFQAV
                     LISNASVELARFIDAVHPIHRDVKSSAYLGDLLVTAYSKFSRNRTFGYMIGKGYSVKA
                     AQLEMEMIAEGYYAVKSIVEMNKNYNVEIPIVQCVYNILYEKIAPAVEVRILTDQLS"
BASE COUNT         1077 a          902 c          883 g         1017 t
ORIGIN      
        1 agagtccacc caaggaaatt ctggatctgg tcgatataca accaacgcca atggttaggg
       61 tttcttcaaa gggttcctat atggtcttgt tggagcggta cgttttcaaa accctcgaag
      121 aactctcgga agaggaattg cgcctggcag gattgcgtat caatccccgt aattttggcc
      181 cttcccgtgc taactacgcg cattccctcc gcatcatgaa catgaacacc ggaaaagcga
      241 tcacagtgaa tggagcgcct tccaatgcgc gtattggctc tctttccatt tctagggacg
      301 aaaaatatgc tgcatttacc aatacaactg ccgagcgtat cgagttatgg atcatggagc
      361 tggtcagtgg taaatgcact ttactaacat ccggtcttgc agtgaatgct gtattgggat
      421 cgccgtattc ctggtcgtcg gataataaag gactctattg caaactcgtc accgatcagg
      481 ttccgctgag ttccatttac cgcaaaccac ttcctgaagg accggtagtg caggtttcgg
      541 gagggaaaaa agcgccggtg cgtacgtttc aggatttgtt acgcaacaaa aacgatgaaa
      601 aattattcga gtcgttcgct gcatccgaat tagtgtattt cggagtggat ggttcccgac
      661 gcaagttcgc ttcacgtgga atgtactatt ctgccgatga atctcccgat ggaaaatttg
      721 ttttacttac ttccattcaa tatccgtttt cttacatcgt tcagctcgat cgttttccgg
      781 taagttatgc agtgcatgaa gcatccggga aattcctgaa cgagttttac cgcaaaccgc
      841 ttattgaaga attaccaaat ggatttgatg cggtagaaac cggcaagcgt gatatcgaat
      901 ggagaaacga tcagaatgcc acactcgttt gggtggaagc acaggatggc ggtgatcctg
      961 caaaagaagt tgcgattcgc gatcatgtat accaggtcga agctcccttt accggtgctc
     1021 cgcaattgct ggcaacaacc gaattgcgtt tttcaggact gagtttcgga aacgcatcca
     1081 tgatgctggt gcgcgaaaac tggtggaaat cgcgacggac aaaaacaacg atggtcgatc
     1141 cgacaagcaa atcaaaaccc aaagtcattt ttgatcgctc cgaggaagat gtgtataacg
     1201 atcccggaaa ttttttgatg gattacaatg aaaacgggca ctatgtgttg cgtacttcaa
     1261 aggacaaaaa gaaattgtac ctcagcggac aaggttattc tcctgcaggg aatcgtcctt
     1321 ttctggatga atacaccatt gccgacggaa aacgcaaacg cctctggcag gcggatggaa
     1381 aaaccaccta tgaaaacatt gctacagttt tcgatccgga aaagcagatc atgctcacta
     1441 cggttgaatc gccggagatg aatcccaact tttacctgcg cagcgggaag tcggcgaaaa
     1501 agatcaccaa tttcccgaac ccctatgcca gttttatggg cgttaccaag caacgtattt
     1561 tttacaagcg caaggacgga gtagaccttt cggctacctt gtatttaccc aagggctacg
     1621 ataaagtgaa agatggtccg ctccctgttg ttatgtgggc ctatccgcgc gagtttaaag
     1681 acaaagcaca agccggccag gtgaaggagt cgccgcatcg ttttgcgcag atcaattacg
     1741 gatctcccgt gtattgggcc atgcgtggtt atgccattct cgacaataca gatttcccca
     1801 ttgtgggcga aggaaaaaca gaacccaacg attcgtttat cgaacaactc gtagccaacg
     1861 ccgaagcagc catcaataca ctggcagaaa tgggtgtggg cgacagaaaa cgtgtggcga
     1921 ttggcggaca ttcctacggt gcattcatga cggcaaatct catggcgcat tgcgatctct
     1981 ttgccgcggg aattgcacgc agtggtgctt acaaccgaac actgactcca ttcggatttc
     2041 aagctgagga acgcacgttc tgggaagcga aaaaagtgta catggagatg tcgccttttt
     2101 atttcgctga aaagatcaat gaacctcttc ttctcattca cggagatgcc gataacaatc
     2161 ccggcacatt caccctgcaa agcgaacgtt tgtttgcagc cattaaagga ctgggaggaa
     2221 cggcacgatt ggtattgttg ccctacgaaa gccacggcta ctctgcccgc gaaaacatac
     2281 ttcacatgct ctgggaacaa gaccagtggc tggaggctta tgtgaagaat aagaagtgaa
     2341 ttatttaaaa ccacttcctc cttcggcgga ctagaaggca ctaagcgcac ttagaaccac
     2401 aaagaaactt agtgaacctt tgtgtcctta gtgccttaat ggtgaaagaa aatcaggata
     2461 attggtccgt caaaattctc acctcaacag cgggagcaat tttttcgtaa aggatattgt
     2521 acacgcactg cacaatggga atttccacat tgtagttctt gttcatctcc acaatgcttt
     2581 ttacggcata atacccttct gcaatcattt ccatttcgag ttgcgcagct ttaacggagt
     2641 atccttttcc aatcatatag ccgaacgtcc ggttgcgcga aaatttagaa taggcggtta
     2701 ccaataaatc tccgagataa gcactcgatt ttacgtcgcg gtgtatggga tgaacggcat
     2761 caataaaacg cgcgagttcc accgatgcat tcgaaatgag cacagcctgg aaattatcac
     2821 cgaaaccgag tccgccgcaa attcctgaag caatggcgta aatgttttta agtactgcag
     2881 ataattccgt accgaacaga tcatccgaaa ccgttgtgcg gatataccgg caagccagca
     2941 ggtcggccat gtgttgtgca accgatgtat cgctgcaacc aatggtgagg taactcaatc
     3001 gctcgagagc cacttcctcg gcatgacagg gtccgcaaat gattccgatt ttctcgtagg
     3061 gtacctgcag ctgttctttg aaataggtag caggaatggc gttgtattcg ggaaccattc
     3121 ccttgatggc agaaaaaata atttttcctt tgaaattctc tgcaggatat ttttggaaca
     3181 ccgaatgcaa aaacgcagcg ggtgttgcca taattaaaat atcgcatttt ggaatgatat
     3241 cctccagtcg gttggtaact accaattgac cggggttgaa ttcaaccgac tgcaaatagc
     3301 gtggattatg atggaaatta cgaatatggt ccacactttc tttcttgtga acccaccaat
     3361 aaacagtatc aacctgattg cataataatt tgacgatcgc tgttccccag ctacctccgc
     3421 caataacccc tattttcacc tgctttgcat tcatgacctg acgaatgtac gaaatcagtt
     3481 gattttctta agtgcttcat tctcaaaatc gaaaattttc agaaaacgac gttcgtccta
     3541 aatttgagca tttgcaaaca ccttcgcgca tatttcctgc atatttgtat tggaaaacta
     3601 ctgcagactg aactaagaaa attccattct tgggcttttt cgcttttttg aaggtattaa
     3661 gggctttcgg tgcctcgtac gttctttcct cattcgccat gcaatggggt gccggaagcc
     3721 ttctctttcc taaacgattt gttaaaacat gaggcacccc gcaaatctgt ggtaggttcg
     3781 ccatagaaat ttattcccat gaaaaacaga ttgtacctcg tcgcactatt ccttgtgcat
     3841 tcactcctaa caattgcaca gacaaaggaa atgaaagtc
//
LOCUS       DAOMYG010000308         3857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8426_length_3857_cov_8.200947, whole genome shotgun sequence.
ACCESSION   DAOMYG010000308 DAOMYG010000000
VERSION     DAOMYG010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8426_length_3857_cov_8.200947"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PK610_09980"
     CDS             complement(<1..882)
                     /locus_tag="PK610_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRJ36306.1"
                     /translation="MKSTQLRVSEMAENLIGSEIIKLAGEIREKIAKGEKIHNFTIGD
                     FNPKIFPIPTALRDAIIEAYQNDETNYPEANGMAALRKSVAAFTNERMGLNYSENEFL
                     ISGGARPIIYAVYRTLIDPNDKVVFPVPSWNNNHYSHLTSSQCVMIETTPENYFMPSA
                     DELRPHIRDAAMIALCSPLNPTGTVFSKEGLESICDLILEENARRGPEQKPLYLMYDQ
                     IYWVLTYGSTKHYDPVSLRPEMRNYTIFIDGISKSFAATGVRVGWAFGPLRVIDKMKS
                     ILSHVGAWSPKAEQLATA"
     gene            complement(941..1555)
                     /locus_tag="PK610_09985"
     CDS             complement(941..1555)
                     /locus_tag="PK610_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sprT domain-containing protein"
                     /protein_id="HRJ36307.1"
                     /translation="MMTERDLVGKLEVYLPPGTSETIARWIARSGVHFRITMPRNSVY
                     GDYLHPHAGKGHRITVNGNLNAYSFLVTTVHEFAHLECWNKHRNYVKPHGEEWKKEFK
                     ILMHPLLESRIFPRDIDLALRKYMADPGASSCSDPDLIKALRNYDRNKMLMLDELPEG
                     GRFRFGDRDFKKGQKRRTRYECVEISTRMVFLISGTAEIEVLGD"
     gene            complement(1559..1708)
                     /locus_tag="PK610_09990"
     CDS             complement(1559..1708)
                     /locus_tag="PK610_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36308.1"
                     /translation="MKRRVIRLAILALVAVSVSSCGLFRKKNKCHTCPHWSYEQSAND
                     GQFRG"
     gene            1790..2539
                     /locus_tag="PK610_09995"
     CDS             1790..2539
                     /locus_tag="PK610_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ36309.1"
                     /translation="MGIKIFIEEIGRYYLFMRAVFRRPEKMKIYYGRFLDECIALGIN
                     SLGIVSIMSLFIGAVITLQTSYNIDSPFIPLYTVGFTTRQSIILEFSPTIISLILAGI
                     VGSNISSEIGSMRVTEQIDALDIMGVNSAGYLVLPKVLGAMFMFPFVIVISMFLGIFG
                     GWLFGTMTGQITTHVYLYGIQYWFDPYSIQYALTKTVAFAFIITSIASFYGYYTSGGS
                     VEVGRSSTRAVVISSIFILLANYLLTQWLLL"
     gene            2536..3294
                     /locus_tag="PK610_10000"
     CDS             2536..3294
                     /locus_tag="PK610_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ36310.1"
                     /translation="MIEIRNLSKAFNGIPILEDVSVDFQPGVINMIIGQSGSGKSVFT
                     KCIVGLLNADSGSILYSGKDFASMSDKQRLDIRQDIGFLFQYSALFDSLTVEENVMFP
                     LRLFSKMSREEMLDRVNFCLKRVNIENKNHLFPSEISGGMKKRVGIARAIAMNPKYLF
                     CDEPNSGLDPQTSIVIDNLIEEITHEFNITTVVITHDMNSVIEIGQNIIFLYKGKKWW
                     QGGKDDIIRTDNPEIIDFVYASKFMKTLKDKLAK"
     gene            complement(3499..3648)
                     /locus_tag="PK610_10005"
     CDS             complement(3499..3648)
                     /locus_tag="PK610_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36311.1"
                     /translation="MMRRWMKQLMNWGTMPITNPVNEDLWTQRLNEQIREGRPVQMNG
                     HWFMI"
BASE COUNT         1058 a          865 c          835 g         1099 t
ORIGIN      
        1 tgcagtggca agttgttcag cctttggcga ccacgcacca acgtgtgata gaatggattt
       61 cattttatcg atgacacgca atggaccaaa tgcccatcct acacgtacac ccgttgctgc
      121 aaacgatttt gaaattccgt caatgaaaat ggtgtaatta cgcatttccg gacgcaacga
      181 aaccggatca taatgtttcg ttgaaccata agttaaaacc cagtagatct gatcgtacat
      241 caaatacaaa ggcttctgct ccggtccgcg acgcgcattc tcttcgagga tcaggtcaca
      301 gatactttca agtccttctt tgctgaaaac agttccggtt ggattgagcg gagaacacaa
      361 tgcgatcatt gctgcatccc tgatgtgcgg acgaagttca tctgccgaag gcatgaaata
      421 attttcaggt gtggtttcta tcatcacaca ctgagaagaa gtaagatgag aatagtgatt
      481 attgttccag gagggaacag gaaaaactac tttgtcgttc ggatcgataa gtgtacggta
      541 tactgcataa atgataggac gcgcaccacc ggagatgaga aattcgtttt cagaataatt
      601 aagtcccatc cgctcattgg taaacgcggc aaccgatttg cgcagcgcag ccattccatt
      661 tgcttcggga taattggtct catcattctg gtacgcttca atgattgcgt cacgaagagc
      721 agttggaatg ggaaagatct tcggattgaa atcgccgatg gtaaaattgt ggatcttttc
      781 acctttggct attttctcgc ggatctcacc ggcaagtttt atgatttccg atccgatcag
      841 gttttctgcc atttcggaaa cgcgcagttg tgttgatttc atttcagtgg attgcatcac
      901 agttttgttc ttgccgcgaa ggtagaaaaa agggggtatc tcagtccccc aaaacttcaa
      961 tttcagcagt accactgatc agaaagacca ttcttgtgga aatttcaacg cattcgtatc
     1021 gtgttctgcg cttttgacct tttttaaaat cccgatcacc aaagcgaaat cgtcctccct
     1081 caggtaattc atcgagcatc agcatcttat tgcgatcgta gttccgcaat gctttgatca
     1141 agtctggatc gctgcaactc gatgcgcccg gatcagccat gtatttacgt aaagctaaat
     1201 cgatatcgcg ggggaaaatc ctgctttcca aaagagggtg catcaatatc ttgaattcct
     1261 ttttccattc ttcgccatgt ggtttcacgt aatttctgtg cttgttccaa cattccaggt
     1321 gggcgaattc atgcacagtg gtaaccaaaa acgaataagc attcagattg ccattcaccg
     1381 taatgcgatg tcctttaccg gcatgcggat gaagataatc gccgtaaaca gaatttcggg
     1441 gcatggtaat acggaagtga acaccactgc gtgctatcca acgcgcaatg gtttcggaag
     1501 tgccgggtgg aaggtacact tccagcttcc cgacgagatc ccgctcggtc atcattgctt
     1561 atcccctgaa ctgaccatca tttgccgact gctcgtaact ccagtgcgga caagtatgac
     1621 atttgttctt cttacggaac aatccgcagg aggagacaga aacagccaca agggcaagaa
     1681 tggcaagacg tattactcga cgtttcatac cgtaaaaata cgaaataagc gtgccaaagt
     1741 caacttactc ttagttacgt tggaaaatcg tattttcgac cactgattta tgggtatcaa
     1801 aatctttatt gaagaaatcg gacggtatta cctctttatg agggccgtgt ttcgccgtcc
     1861 cgaaaagatg aagatttact acgggcgttt tctcgacgaa tgcatcgctt tgggaatcaa
     1921 ttcgctggga atcgtatcca tcatgtcgct ctttatcggg gctgtaatta ccctgcagac
     1981 atcgtacaat atcgactctc ctttcatccc gctttatact gttggtttca caacacggca
     2041 gtccatcatc ctggaattct cgccaaccat tatttcattg attcttgcag gaattgtagg
     2101 ctccaacatc tcatccgaaa tcggatcgat gcgggtaacg gaacaaatcg acgcattgga
     2161 tattatgggg gttaattccg caggatatct tgttttgcca aaagtactcg gcgccatgtt
     2221 catgtttcct tttgtgatcg tgatctccat gttcctgggt attttcggcg gatggttatt
     2281 cggtacaatg accggacaaa tcaccacaca tgtttacctg tacggaattc agtattggtt
     2341 cgacccctac tctattcagt acgcattgac aaaaacagtt gcctttgcct ttatcattac
     2401 cagtatagcc tctttttacg gttactacac ttccggtggt tcagtagaag ttggacgatc
     2461 cagcacacgc gctgttgtta tttcgtccat attcattctt cttgccaact acctgctaac
     2521 tcagtggctg cttctatgat cgagatcaga aacctcagca aagcgttcaa cggaattcca
     2581 attctggaag atgtcagcgt ggattttcag ccaggggtga tcaatatgat tattggtcaa
     2641 agtggttccg gtaaatctgt atttaccaaa tgtattgttg gattattgaa tgcagattca
     2701 ggttctattc tatacagtgg aaaagacttt gcctcgatga gtgacaaaca acgtttggat
     2761 attcgtcagg acatcggatt tctatttcag tacagcgcct tgttcgattc gcttactgtg
     2821 gaagaaaacg tgatgtttcc attgcgtttg ttttcgaaaa tgtcgagaga agaaatgctc
     2881 gatcgggtga atttttgttt gaagcgtgtt aacattgaaa acaaaaacca tcttttcccc
     2941 tccgaaattt ctggtggaat gaaaaaacgt gttggtattg cacgtgccat cgccatgaat
     3001 cccaaatacc ttttttgcga cgaacccaat tccggtcttg atccgcaaac gtccatcgta
     3061 atcgataatc tcattgaaga gatcacgcat gaattcaaca ttaccactgt tgtgattacg
     3121 cacgacatga actcggtaat tgaaatcgga cagaacatta ttttcctgta taaaggaaaa
     3181 aaatggtggc agggaggaaa agacgacatc atccgcaccg ataatccgga gatcattgat
     3241 tttgtttatg cttcgaagtt catgaagacg ctgaaggata agttagcgaa atagcacact
     3301 cttcaccact aagacactga gttcacaaag aacgactaag cccccctttt ttctttttcc
     3361 aaaaaacgaa gaaccccgat accttagtac cggggtcctt ccccaaacaa aaccaaatat
     3421 gcagtccctt ttaccggacc ttattgagca attactcttt tccgcatgcg gctgcggtgt
     3481 gtagagaata aaaagagcct atatcataaa ccagtgacca ttcatctgaa cgggtcgtcc
     3541 ttcacggatc tgttcgttca aacgctgggt ccacaggtct tcgtttacag gattagtaat
     3601 gggcatcgtt ccccaattca tcagttgttt catccagcgc ctcatcataa tctgttgttt
     3661 aggcaaattt acgacgacgg ttacgctaaa gttgggcggt ttaggcagcg gggcacaagg
     3721 ggcaaccact ggtggaaaat accgagggaa tggaaatagt gattttaata caggcccaaa
     3781 ctttacaaca gcagagacaa aatacgcact tgtaaattat acggatgaaa attcccgaga
     3841 atgatttgaa tatctaa
//
LOCUS       DAOMYG010000309         3855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8433_length_3855_cov_5.915000, whole genome shotgun sequence.
ACCESSION   DAOMYG010000309 DAOMYG010000000
VERSION     DAOMYG010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8433_length_3855_cov_5.915000"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PK610_10010"
     CDS             complement(<1..182)
                     /locus_tag="PK610_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36312.1"
                     /translation="MIATEDYPLSRRLFLYSAAEQSNAWAAALIRFAHSPRGQAIVEQ
                     SGFIAQTVQAMQVSSEP"
     gene            293..1225
                     /locus_tag="PK610_10015"
     CDS             293..1225
                     /locus_tag="PK610_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRJ36313.1"
                     /translation="MKNLPINNRHFEGLLREFQRVIRVKGYSRGKNTMYPACAREFLF
                     FIENRDIVDIKDVKAGDVISYYEYIRERPNQRREGGLSESMIRHHLFSLRLFFDFLMD
                     AGIRDSSPAHLPKFQIGKYKEREILTEEEIKEVYKACNDKRDRALIAAAYGCGLRRSE
                     ITKLNVGDIVFHQGTLTVRDGKFGKSRIVPLSDNVIRDLKEYLINERPKRITAKTNAH
                     VNAFFLNNIGTRPAGDYMAKMLRRILDRTQNPNILRKEITLHCLRHSIATHLLDRGAT
                     MEFVQEFLGHSEIDTSMLYSKRRKQRLKIQQHFQ"
     gene            1363..2100
                     /locus_tag="PK610_10020"
     CDS             1363..2100
                     /locus_tag="PK610_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRJ36314.1"
                     /translation="MVVLEEIAKQKPNTRYRIAILSVIKKYCDYLIEIGQRDDHPCKT
                     IQIKFNRDKSVIQQDLFSSTELELLMNREERYPDLKMKNQAVISLLIYQGLSSGEIAN
                     LKVQHIHLDEGKIFVKESRDLMRRHLEMHPKQYRIFDRYINESRKSLLKAETDKLIIG
                     VRGNPITTGEVGYLVEQFKILFPGRNLNPSTIRQSVISNWLNEKKYPLEMVQLMAGHR
                     WISTTQKYRHTSNDEKRILINRFHPLG"
     gene            2146..2532
                     /locus_tag="PK610_10025"
     CDS             2146..2532
                     /locus_tag="PK610_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36315.1"
                     /translation="MEESIEMQFEYVIRRAHHCGRYGAAGADADLYRGFMGCYFTMNE
                     SISKKKPDAIQTGIEYGVRLGKVRMSLDIASKKVIAKIENTNHTDAERIEEIREKIHS
                     ADEPKKMAEAVTELDQLFLKLGLFPG"
     gene            2651..3751
                     /locus_tag="PK610_10030"
     CDS             2651..3751
                     /locus_tag="PK610_10030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRJ36316.1"
                     /translation="MFRQNHTSQFSTLLPQYTESYRYGFNGMEEDNTLKGPGNSYDFG
                     ARMYDSRLGRWMSVDRHDWRYPGVNPYNFAFNIPTVFNDPDGKDGRLTIDYTNKTITL
                     QTTVHLFGASSAEEGHSIAASFNEQFKAGTNEYYIVDREDPTVTWLVKIDIDYVYNDC
                     IDEYNATLNVNDRIVEIKGGEFEKLQGLDIGSGSDLCLNPGDNILLLSNKEPEEIGGV
                     YYLGYVSSIGSNESQIVTKEFKTAMHEVFHMLGYDERYKPGGIPIPYYVNDYLTSGGG
                     QAKEIMPFHFVDLCDFASDFCHGSYEGTHIVGTKPMTETMVTVQNPDGSQSISTQHAP
                     HKLFNIDNTDGGRKVDVTKDKWNTENRVVTRE"
BASE COUNT         1234 a          714 c          888 g         1019 t
ORIGIN      
        1 ggttcgcttg agacctgcat ggcctgcacc gtctgcgcaa taaacccgct ctgctcgacg
       61 atggcctgac cccgcgggct atgggcaaaa cggatcagtg ccgctgccca ggcattgctc
      121 tgctccgcag cgctgtagag aaacagtcgg cgtgacagag gatagtcttc cgtggcgatc
      181 aacgctgcgc tagacccgca attatcaggg gaatgagcgg tggtggttca ctattccaag
      241 ggtacgcatt gtataggaga aaaactgatc gtaacgaaat agaaaaacaa aaatgaaaaa
      301 cctacctata aacaacaggc attttgaggg gcttttgcgc gagtttcagc gcgtgattag
      361 ggtgaagggg tattcacgcg gaaaaaacac gatgtacccc gcctgtgcgc gtgaatttct
      421 attctttatt gagaaccggg atatagtcga tatcaaagac gtaaaagcgg gggatgtaat
      481 ttcctattac gaatatatcc gtgaacgccc gaaccaacga agggaaggcg ggcttagtga
      541 aagcatgatt cgtcatcact tattttcact gcggttattt tttgattttc tgatggatgc
      601 cggaataaga gattcttcac ccgcgcacct tccgaaattt caaatcggaa aatacaagga
      661 gcgggaaatc cttactgaag aagaaataaa ggaagtttat aaggcttgca acgataagcg
      721 tgatcgcgca ttaattgctg ctgcttacgg ttgcggactg cgcagatctg aaattaccaa
      781 actgaacgtg ggtgatattg tttttcatca gggaacgctt accgtgaggg acggtaagtt
      841 tggaaagagc cggattgttc cgctttctga caacgtcatc cgcgacctga aggaatacct
      901 gatcaatgaa cgcccaaagc gcattaccgc caaaacaaac gcgcacgtta acgctttttt
      961 cctgaataat atcggaacaa gaccagcggg tgattacatg gctaaaatgc tcaggcgaat
     1021 tcttgaccga acgcaaaacc cgaatattct acgcaaggaa atcacacttc attgtttgcg
     1081 ccatagcatt gccacccacc ttttagatcg cggggcaaca atggaatttg tgcaggagtt
     1141 tttaggccat tcggaaattg atacctcgat gctctattct aaaagaagga agcagcgact
     1201 caaaatacag caacactttc aatgaaacag gaggaattga catttgaaaa gtatttatac
     1261 cggatcggtt tcacgaacca gtccgtgaaa agttacttgt atcaattcga aaacttcata
     1321 acggtgtatc cggatgcagc gcgattcagt tataaggaca ttatggtcgt actggaagaa
     1381 atcgccaagc aaaaaccaaa tacgcggtat agaatcgcca ttttatcggt aatcaaaaaa
     1441 tattgcgact acctgattga aatcggccag cgggatgatc atccttgcaa gacgattcag
     1501 ataaaattca accgtgacaa atcggttatt cagcaagatt tgttttcatc tactgaactg
     1561 gagcttttaa tgaaccgcga ggaacggtat cccgatctga aaatgaaaaa tcaggcggtg
     1621 atttcacttt taatttacca agggctttca tccggtgaaa ttgcaaattt gaaagttcag
     1681 catattcatc ttgacgaagg aaaaatattt gtgaaggaaa gcagggactt aatgcgtagg
     1741 catttggaaa tgcacccgaa acaataccgg atttttgatc gctacataaa cgagtccagg
     1801 aaatcgctgc tgaaggccga aaccgacaaa ctgattatag gtgttagggg aaatccgatc
     1861 accaccgggg aagtcggtta tttggtggag caattcaaaa ttttgtttcc cggtcgcaac
     1921 ctgaacccaa gtacgatccg gcaaagcgtg atttcaaatt ggctcaacga aaaaaagtac
     1981 ccgctcgaaa tggtgcagct aatggcaggc caccgatgga tttcaactac tcaaaaatat
     2041 cggcacactt ccaatgacga aaagcgaatt ttgataaatc gatttcaccc attgggatga
     2101 aacaaaatta gaaaaccatc gtataaaggt gaaaataaag tatttatgga agaaagtatt
     2161 gaaatgcaat ttgaatacgt aattagaagg gctcaccatt gtgggcgtta tggggcagca
     2221 ggtgctgacg cggacttata tcgaggtttc atggggtgtt acttcactat gaatgagtca
     2281 attagcaaga aaaaacccga tgctatacag accggaattg aatatggagt ccgacttgga
     2341 aaagttagaa tgtctttgga tattgcatcg aagaaggtca tcgctaaaat tgaaaatacg
     2401 aatcataccg atgcagaaag aatcgaagaa attagggaaa aaatacattc tgccgatgag
     2461 ccgaaaaaaa tggctgaagc tgtaactgaa ttggatcaac tatttttaaa actcggctta
     2521 tttccgggtt agtttaataa aactaactcg gcttatttcc gggttagttt tattaatatt
     2581 ttcattgtaa tggggagcca acggctccct tttttcatta tatttggtac aatccacgag
     2641 tcaaaactga atgtttcgcc aaaaccatac gagtcaattt tctactttac tcccacagta
     2701 cacggagtcg tatcgctatg gtttcaacgg aatggaagaa gacaacaccc tcaaaggccc
     2761 cggaaacagc tacgattttg gtgcgaggat gtatgatagt aggttgggga gatggatgag
     2821 tgtggataga catgattggc gttatcctgg tgttaatccg tataattttg ccttcaatat
     2881 cccgacagtg tttaacgatc cagatggaaa ggatggtcga ttgacaatcg attatacaaa
     2941 caaaacaatt acactacaga caactgttca cctatttgga gcaagttctg ctgaagaggg
     3001 tcattcgatt gccgccagtt tcaatgagca atttaaagcc ggaacaaacg aatattatat
     3061 tgtagatcgt gaagatccga ctgtcacatg gctagtgaaa attgatatag attatgtata
     3121 taatgactgt atagatgagt acaatgcaac gttaaatgtt aatgatagga ttgttgaaat
     3181 aaagggagga gaatttgaaa agctgcaagg attagacata gggtcaggaa gtgacttatg
     3241 ccttaatcct ggtgataata ttttattgct ttcaaataag gagccagaag aaataggtgg
     3301 agtgtactat cttggttacg tttcctcaat aggtagcaat gaaagtcaaa tagtaaccaa
     3361 agaatttaaa actgctatgc atgaagtatt tcatatgctt ggttatgatg aaaggtacaa
     3421 gccaggagga attccaattc cctattatgt taatgactat ctgacatcag gaggtggtca
     3481 agcaaaagaa attatgccat tccattttgt ggatttgtgt gattttgcat cggatttctg
     3541 tcatggtagt tatgaaggaa cacatattgt tggtaccaaa cctatgacag aaaccatggt
     3601 tacggtgcaa aatcctgatg gaagtcaatc gatatcgacc caacatgcac cacacaagct
     3661 atttaatatt gataacactg atggtggaag aaaggtggat gtgaccaaag ataaatggaa
     3721 tacggagaac agagtcgtta caagagaata atggaaacta aatttgttga tgttcatttt
     3781 agttgtattg atttattcga cttatttcta gggtagaatt attttctaga aactgaaagc
     3841 gatttttagt agggt
//
LOCUS       DAOMYG010000310         3851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8446_length_3851_cov_11.399104, whole genome shotgun sequence.
ACCESSION   DAOMYG010000310 DAOMYG010000000
VERSION     DAOMYG010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8446_length_3851_cov_11.399104"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3063
                     /locus_tag="PK610_10035"
     CDS             <1..3063
                     /locus_tag="PK610_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HRJ36317.1"
                     /translation="AQASDFSITSAHTYPFTIASGSSVTVDVRFLPTATGNRNATFTI
                     SNNDYDERTFDFAIAGFGNSQTTSVSSSESPYVIPTAPGVTTSAVLTVGDQIGAYRMV
                     GIPDGLGAYDNGDATFTLLMNHELGTTQGIARAHGNTGAFVSKWIINKNTMQVLSGSD
                     LIQTVYLWNGSTYLLYNASTPMPSGFGRFCSGDLADVNAFYNPNNGKGTQNRIYLTGE
                     ENGADGRAFGMLATGPDAGKAWELPYMGKFSWENAVASPKVQDKTIVVGLDDATGGQV
                     YVYIGNKSTSGNDIQKAGLNGGALYGIVVSGLPTEVSGSFIAPNTPFTMVNMGTVHNI
                     TGATLETNSTAQNVTKWLRPEDGAWDPMNPNDFYFVTTNAFNSPSRMYRLRFTDINNP
                     ELGGTVTAVLDGTEGPQMMDNLDMDHFGNILIQEDPGNQTYLAKIWQYNVETDVITEI
                     AAHDADRFTTTGLNFLTTDEECSGIIDMQGILGAGYFVMVVQDHYNISGELVQGGQLM
                     TLFNPGTFHANPEISVAGNAVGISDGDNTPAIADHTDFGSVTVQTSFSRTYTIHNNGP
                     GALKLTGASVSGANAADFTFAGLPVFPATIAAGSSVSFQVNFTPSAIGLRTATVTLLN
                     NDYTESEFTYAIQGTGICSVPNATITPQGSTTFCPGSSVILDAPAASSYLWSNGATSQ
                     SISVSAQTNYFVVVTDANGCTATSAPVAVSVFSQPQVNISTTTGTLNVCSGSSLTLSA
                     NNAASFVWSTGATTQSVNVTTAGTFSVQITDNNGCTNSMSVTTTLVPSPNVTASVSAT
                     TICQGESVTFTGNGAQSYSWNNGVTNGLPFTPSATQTYIVTGSAANGCTDTDQVTVTV
                     NPIPAIPVVTANGTTLTSSAATAYQWYLNGSTIGGQTGQSIVVTNNGTYMVVVTNASG
                     CSNQSAPFAFNSVGVEEQSFINRIEFFPNPFSGNATIRLYAMESSQAVITLTDVTGKL
                     VMNKTVTLVAGVNEIPMSEKEFAAENGNYFLTVEIGGKTQTIKAIKID"
     gene            complement(3141..3824)
                     /locus_tag="PK610_10040"
     CDS             complement(3141..3824)
                     /locus_tag="PK610_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36318.1"
                     /translation="MLLELWGRYIYPLALTIFYIFIGTKIDYFTFSYLEKERQKKIEK
                     KLEIQKSYAVSGETHINALKKIIEEQKAFLELKKESIESLSKIESLNLKVSNLESEKI
                     DDANSIYNLKNSIEKFYQSTDITKHISGKWKVTLSTNNHGHSTLYYDINPHSIQITQN
                     MSPFGNYTISHFFRHPNSNKIYIISFDSTSTGRTPLILDLTYISSKFLSGSQNSISLI
                     EMEKMIDDD"
BASE COUNT         1073 a          753 c          793 g         1232 t
ORIGIN      
        1 gctcaggctt ctgatttttc aattacaagt gcgcacactt atccattcac cattgcttcc
       61 ggtagttcag ttactgttga tgtacgtttt ttgcctactg ccaccggtaa ccgcaatgca
      121 acatttacca tttccaataa tgattatgat gagcgaactt ttgatttcgc tatcgcaggt
      181 tttggaaatt cacagaccac tagtgtcagc agttctgaat ctccatatgt tattccaact
      241 gctccgggag ttacaacttc tgcggtactt actgtaggtg atcaaattgg tgcttatcgc
      301 atggtgggaa ttcctgacgg acttggtgct tacgacaatg gagatgcaac ttttactctt
      361 ttgatgaacc atgaacttgg cacaacacaa ggtattgcac gtgctcatgg taatacaggt
      421 gcgtttgttt ctaagtggat cattaacaaa aacaccatgc aggttttgag cggatcagat
      481 cttattcaga cagtgtacct ctggaatggt tcaacctatt tactttataa tgcgtcaact
      541 ccgatgcctt caggttttgg acgtttttgc tccggagatc ttgctgatgt aaatgcgttt
      601 tataatccga acaatggaaa aggtactcag aatagaatct accttaccgg agaagaaaat
      661 ggtgcagacg gaagagcgtt tgggatgctt gcaaccggac ctgatgctgg aaaagcatgg
      721 gaacttcctt acatgggtaa attctcctgg gaaaatgctg ttgcaagtcc aaaggtgcag
      781 gataagacca tcgttgtcgg acttgatgat gctacaggtg gtcaggttta tgtttacata
      841 ggaaacaaat ctacttcagg gaacgatatt cagaaggctg gtcttaatgg cggagcgttg
      901 tatggtatag ttgtttcagg attgcctact gaagttagcg gaagcttcat tgcacctaac
      961 actccattca ccatggtaaa catgggaaca gttcacaaca ttacaggagc aacattggaa
     1021 actaactcca cagcacagaa cgtaacaaag tggctgcgtc ctgaagatgg tgcatgggat
     1081 ccgatgaatc cgaatgattt ctactttgta actaccaatg ctttcaatag tccatctcgt
     1141 atgtatcgtt tacgttttac ggatatcaat aatcctgaat tgggtggaac agtaactgct
     1201 gttttggatg gaactgaagg tccacaaatg atggataatc ttgacatgga tcattttgga
     1261 aatattttaa tacaggaaga tccgggtaac cagacttacc ttgcgaaaat ctggcagtac
     1321 aatgtggaga cagatgttat cacagaaatt gctgcacatg atgccgatcg ttttactaca
     1381 actggtctaa acttcttaac cacagatgaa gaatgctctg gaatcatcga tatgcaaggt
     1441 atccttggag caggttactt tgttatggtg gttcaggatc attacaatat cagtggtgaa
     1501 ttggttcagg gcgggcagtt aatgacgctc ttcaatccgg gtacctttca tgcgaatcct
     1561 gaaatttcgg ttgcaggaaa tgctgttggt atttctgatg gagataacac acctgcgatt
     1621 gctgatcata ctgattttgg ttctgtaaca gttcagacat ctttctccag aacttatacc
     1681 atccataata atggtcctgg tgcattaaaa ttgaccggag cttcagtttc aggtgccaat
     1741 gctgctgatt tcacctttgc aggtctgcct gtatttccgg ctaccattgc agcaggttct
     1801 tctgtttcgt ttcaggtgaa ttttacgcct tctgcaattg gtctgcgtac tgccacggtt
     1861 actttgttaa acaacgatta taccgaatct gaatttacct acgctattca gggaacgggt
     1921 atttgctctg taccaaatgc aaccatcact cctcagggtt caacaacatt ctgtcctggt
     1981 tcatcagtta tacttgatgc tcctgcggca agttcttacc tatggtcgaa tggtgcaact
     2041 tctcaaagca tttctgtttc tgctcaaacc aactattttg ttgtggtaac agatgcaaac
     2101 ggttgcactg caacatctgc tccggtagct gtttcagttt tctctcagcc gcaggttaat
     2161 atttcaacca ccacaggaac gctcaatgtt tgttcaggtt cttctttgac cctttcagcg
     2221 aacaatgctg cttcttttgt ttggtcaaca ggtgcaacca cccaatcggt gaatgtaacc
     2281 acagccggaa ctttttcagt tcagatcact gataacaatg ggtgtacaaa ttcaatgagt
     2341 gttactacca ctctggtgcc atcgccaaat gttacggcat cagtaagcgc aacaactatt
     2401 tgtcagggcg aatctgtaac ctttacaggt aacggtgcgc aatcgtattc ctggaacaat
     2461 ggggtgacca acggtcttcc gtttacgcct tcggcaacac agacatacat tgttaccgga
     2521 tctgctgcaa atggatgtac ggataccgat caggttactg ttactgtaaa tcctattcct
     2581 gcaattccgg ttgtaactgc caatggaact actctaactt catctgcagc tactgcatac
     2641 cagtggtacc ttaacggttc aaccataggt gggcagacag gtcaatcgat agtagtaaca
     2701 aataatggaa cctatatggt tgttgttacg aatgcctccg gttgcagtaa ccaatctgct
     2761 ccgtttgcat ttaactctgt tggtgttgaa gagcaatcgt ttattaaccg tattgaattc
     2821 ttcccgaacc ctttctcagg caatgcaacc atccgccttt atgcaatgga atcatcacaa
     2881 gccgtaatta cccttacgga tgttaccgga aaactggtaa tgaataaaac ggtaactctt
     2941 gttgccggtg tgaatgaaat tccgatgagt gaaaaggagt ttgctgctga aaatggcaac
     3001 tatttcctga ccgttgaaat cggtggaaaa acacaaacca ttaaggctat caaaattgat
     3061 taatgttagg ttttaagatg aagatccccc ttcggttttc cggaggggga ttttttatcg
     3121 ctggcctgaa attcttaaaa ctaatcatca tcaatcattt tttccatttc aattaatgaa
     3181 atgctatttt gtgatccaga aagaaatttt gaagaaatat atgtaagatc aaggattaaa
     3241 ggagttcttc ctgttgaagt tgagtcaaat gaaataatat aaattttatt agagttcgga
     3301 tgtcgaaaaa aatggcttat tgtatagtta ccaaaaggag acatattttg tgtgatttgg
     3361 atactgtgag gattaatgtc gtaatataat gttgagtgtc catgattatt tgttgataaa
     3421 gtaaccttcc attttccaga aatgtgttta gttatgtcag tggattgata gaatttttca
     3481 attgaatttt taagattata aattgaattg gcatcatcta ttttttcact ttccaaattt
     3541 gatactttga gattcagact ctcaatttta cttaaggatt caatggattc ttttttaagt
     3601 tcaagaaatg ctttctgttc ctcaataatt ttctttagag catttatatg tgtttcccct
     3661 gaaacagcgt agcttttctg tatttcaagt tttttttcaa tttttttctg acgttctttt
     3721 tctaaataag agaatgtaaa atagtcgatc tttgtgccaa taaagatgta aaaaatagta
     3781 agtgctaatg ggtatatgta cctaccccat aattccaaca gcataaaaat ataaattctt
     3841 tttcatttta t
//
LOCUS       DAOMYG010000311         3827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8530_length_3827_cov_7.141569, whole genome shotgun sequence.
ACCESSION   DAOMYG010000311 DAOMYG010000000
VERSION     DAOMYG010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8530_length_3827_cov_7.141569"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PK610_10045"
     CDS             complement(<1..481)
                     /locus_tag="PK610_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36319.1"
                     /translation="MKNIIIGNGVIIQFGGKEYTNESIIKRATKTVKLSKHPSKIYPE
                     EIQPWLEQLFGAIPDILNGRYDRLAVLKGEKEGLDDFKRRYAWVGTKTNNHEIGFEDY
                     FLIHELFCRNLKISNPNKSDFRECLRMFFLDAIFNEGKINQVYHTFPDKFKKDLSSYE
                     "
     gene            complement(565..1491)
                     /locus_tag="PK610_10050"
     CDS             complement(565..1491)
                     /locus_tag="PK610_10050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRJ36320.1"
                     /translation="MSTKNAKTTPAVEVNKTNYLMVKPDLIVIDEKENPRQDYGNIEE
                     LMLSVLENGIRTPLNVYEKNGKYVLKNGFRRMKAVLLAISKGKTIERVPVLLEHTKLN
                     EEERTLEHIINNDGKPLTMLEQSEVIRRLLNFGWKVTDVVKRTGKARGYIENLILLTK
                     APMKVQHYIKDGKISAHAVIQIMQAVKGDAEQTLDAVEEAMKSAAASGKEKATPKHVQ
                     TKEVKNLSFGKFYKWCEDIVSTMAGNDVAIKERTNLIENLLVYFENGRSASNVAEELF
                     TDKTKLKPSTAKTTPTKKSPVKTAKKAPKKKK"
     gene            1849..>3827
                     /locus_tag="PK610_10055"
     CDS             1849..>3827
                     /locus_tag="PK610_10055"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRJ36321.1"
                     /translation="MKREDAKLAIENLVERFSEHRKEYHHADYNEQKVRQDFINPFFK
                     ALGWDVDNERGDSEAYREVIYEDKVVVRGKVKAPDYGFRLAGSGKKRLFYVEAKKPSV
                     PIKTHKESAYQLRRYGSSAQAYVSILTNFEDFVVYDCSRVPNQNDSASVARLKHIHFE
                     DYVKEFDFIYDTFSHEAVTRGRFDKFIQAGASKRGTDTLDKRFVQSLDKWRKYLAVKE
                     FDFIYDTFSHEAVTRGRFDKFIQAGASKRGTDTLDKRFVQSLDKWRKYLAVSIAYNNR
                     KLDDEEVNFSVQQTIDRLIFLRFCEDRAVEPYGQLKSTVAKGESYKNLFDLFKQADDK
                     YNSGLFDFKKDVLTPTLKVENKVIKNIVEELYYPKCNYEFSVMPVEILGNAYEQFLGK
                     VIRVTPARSVKIEEKPEVRKAGGVFYTPQYIVDYIVQHTVGKLIEGKSPKEIEKIKIV
                     DPACGSGSFLLGAFDYLLNYHINWYHQKGYATKKGKDNPFTPQGTLTTHEKKRILLNS
                     IYGVDIDANAVEVTKLSLLLKCMEGETEASIKQQMTMFHERVLPDLDNNIKDGNSLID
                     VDFYEKELDLGFEKKIKPFSWRKGFPDIFKNGGFDVTIGNPPYLGGRDWKEDNGNAYD
                     YFIERYSVAEYQFDMYSLFWERAIKITRDNGFIGY"
BASE COUNT         1120 a          734 c          769 g         1204 t
ORIGIN      
        1 tttcataaga agataaatct ttcttgaact tatcaggaaa ggtatgataa acctgattta
       61 ttttgccttc attaaaaatg gcatcaagaa aaaacatcct tagacattcc cgaaagtctg
      121 atttgttagg attacttatt tttaagttcc gacaaaaaag ttcatgaatc agaaagtaat
      181 cttcaaaacc aatttcatga ttatttgttt tcgttcctac ccaagcatat cttcttttaa
      241 aatcatcaag cccttctttt tcgcctttca aaacagccaa acgatcgtag cgaccattga
      301 gaatatcagg aatggcacca aataattgtt ccaaccaagg ttgaatttct tcaggataaa
      361 ttttagatgg atgctttgaa agttttacag tttttgttgc tcttttaatt atggattcgt
      421 tggtgtactc tttaccacca aactgaataa taacaccatt accgataata atatttttca
      481 taaatctact taacccaaat atacggttaa ataaagaaaa agccccgctg gtcagcgagg
      541 ctttgataat gagtgaagcc cggtctattt cttctttttc ggggcttttt tagccgtttt
      601 aacgggagat ttctttgtcg gggtagtttt agcggttgaa ggctttagct tcgttttatc
      661 cgtaaataac tcctcggcaa cgttgctggc agatctgccg ttttcaaaat acaccagtag
      721 gttttcaatc aggttggttc gctccttaat ggctacatca ttccccgcca ttgtgcttac
      781 gatatcttcg caccatttgt agaacttacc gaaggacaga ttttttacct ccttggtttg
      841 aacgtgcttt ggtgttgcct tttctttgcc ggatgctgct gccgatttca tggcttcttc
      901 taccgcatct aaggtttgct ccgcatcacc tttcacggct tgcatgattt gaatgaccgc
      961 gtgtgcgctt atttttccat ccttgatata atgctgaact ttcatcgggg cttttgtaag
     1021 cagaatcagg ttttcaatat aaccccgcgc ttttccggtt cgcttcacta catcggttac
     1081 tttccatcca aagttcagta atctgcgaat tacctctgac tgctccagca tcgttagagg
     1141 tttaccatcg ttgttgatga tatgctccag cgttcgttct tcttcattca gtttggtatg
     1201 ctccaataat accggaaccc gctcaatggt tttgcctttt gaaatcgcaa gtaataccgc
     1261 cttcatcctt ctgaaaccgt ttttcaaaac gtactttccg tttttttcat aaacgttcag
     1321 tggtgttctg attccgtttt caagaaccga tagcattaac tcttcaatgt taccataatc
     1381 ctgacgcggg ttttcttttt catcaattac aattaggtca ggttttacca tcaggtaatt
     1441 ggttttgttc acttctaccg caggggtagt ttttgcattc tttgtactca taaattctgt
     1501 tttagtaatc ggctcgcacc gtgcttgccg ttacaggcat ggcatcatac cctgtgccac
     1561 gaatcgaatg tttcgcattg attttgagta tattacgaag aaatctagtg ttttccctaa
     1621 aacctggact ttgtgtccgg atttgtccga ttctacttgc agcccatttg ccaacgattt
     1681 ccccgatgtc cgaacattct cggttatttt gccgatttct cctatgaaat tcttgcttaa
     1741 tgcagagaat cagcgtattt gttaaacggg tttaatcagt atatttgacc tacttatcgg
     1801 gaagggaaat actcaccctt actgagttaa ttttacagta tatcattaat gaaacgggaa
     1861 gatgcaaaac ttgcaataga aaatttagtt gaacgcttca gcgagcatcg caaggagtat
     1921 catcacgctg actataacga gcagaaagta cgtcaggatt ttattaaccc gttttttaaa
     1981 gcacttggtt gggacgtaga taacgaaaga ggcgattcag aagcctaccg cgaagtaatc
     2041 tatgaagaca aagttgttgt tcggggtaaa gtaaaagcac cggattacgg ttttcgtttg
     2101 gcaggtagcg gaaagaaaag acttttctac gtagaggcaa agaagccgtc tgttccgatt
     2161 aaaacgcata aagaatctgc ttaccagtta cgcaggtacg gaagcagcgc acaggcatat
     2221 gtttccattc ttaccaactt cgaagatttt gtggtgtatg attgttcaag agtaccgaac
     2281 caaaacgaca gtgccagtgt tgcccggtta aagcatattc attttgaaga ttacgtaaag
     2341 gaatttgatt ttatctacga tacattttcg cacgaagcgg taacaagagg gagatttgat
     2401 aaattcattc aggcgggagc atccaaacgt ggaacagata cgttggataa acgttttgtg
     2461 caaagccttg ataagtggcg caaatatctt gctgtaaagg aatttgattt tatctacgat
     2521 acattttcgc acgaagcggt aacaagaggg agatttgata aattcattca ggcgggagca
     2581 tccaaacgtg gaacagatac gttggataaa cgttttgtgc aaagccttga taagtggcgc
     2641 aaatatcttg ctgtaagcat tgcttacaac aatcgcaagt tggatgatga ggaagtaaat
     2701 ttctccgtgc aacaaaccat tgaccgtttg atcttcctac gtttttgcga agaccgtgcc
     2761 gttgaacctt acgggcaatt aaaatctacc gttgccaaag gagaatcgta caaaaatctg
     2821 tttgatttat ttaaacaggc cgatgacaaa tacaactctg gtttattcga tttcaagaag
     2881 gatgtattaa ctccaacact taaagtagaa aacaaagtca tcaaaaacat tgtcgaagaa
     2941 ttgtattacc ctaagtgtaa ttacgaattc tcggtaatgc ctgtagaaat tcttggaaac
     3001 gcttacgagc agtttcttgg aaaggtaatt cgtgttacgc ctgcacgtag cgtaaaaatt
     3061 gaagagaaac ccgaagtacg aaaagccggt ggcgtttttt atactcctca gtatatcgtg
     3121 gattatattg tacagcatac cgttggtaag ctcatcgaag gcaaatcacc gaaagaaatt
     3181 gagaaaataa aaattgttga tccggcttgc ggtagtggct cgttcctatt aggcgcattc
     3241 gattaccttc ttaattatca catcaactgg taccatcaaa aagggtatgc aaccaaaaaa
     3301 ggaaaagaca atcctttcac accgcaggga acgcttacta cacatgaaaa aaagcggatt
     3361 ctgctaaata gcatttatgg tgttgatatt gatgccaacg cagttgaagt aaccaaactg
     3421 agtttattgc tcaaatgcat ggaaggcgaa actgaagcct caatcaagca gcaaatgact
     3481 atgtttcatg agcgtgtatt gcctgatttg gataacaata tcaaggatgg aaatagttta
     3541 attgatgtcg acttttacga aaaagaatta gacttaggtt ttgaaaagaa aattaaaccc
     3601 ttttcttgga gaaaagggtt tcctgacatt tttaaaaatg gtggatttga tgttaccata
     3661 ggtaatcctc catatttagg aggaagggac tggaaagaag acaacggtaa tgcatatgat
     3721 tattttattg aacgatattc cgttgcagaa tatcagttcg atatgtactc attgttttgg
     3781 gaaagagcaa taaagataac gagggataac gggtttatcg gttatat
//
LOCUS       DAOMYG010000312         3826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8534_length_3826_cov_7.954389, whole genome shotgun sequence.
ACCESSION   DAOMYG010000312 DAOMYG010000000
VERSION     DAOMYG010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8534_length_3826_cov_7.954389"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1821)
                     /locus_tag="PK610_10060"
     CDS             complement(13..1821)
                     /locus_tag="PK610_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36322.1"
                     /translation="MAATIKINMNTISNYYNNRKQILDQEIGETEKKINRITFLRLLL
                     FSCALALGWYGIKNSDSLFVATSAFLFIAFIIAVIKHIKLSNDKDLLKRREKVVANEI
                     VLLEGKASMLNNGSAHEEASSYAHDLDVFGEQSLFHRISRCYSPGGRAKLATILTEPP
                     IENQQIKDRQSAIEELAEKTEFRELLLALMLKNDEDKSPESALYQWSENYSPTGNIKL
                     LYILRIALPLLVTAGWLGTYFTGETNYGLWASGISLSVALVLIRQLQEAHAGLDGLKD
                     QLKTYAQVWKLISEEKFESSATITIQNDLKEAEKAFRELTQISERFDRRLNPLVFVLL
                     NATIAYDLFCATRLHEWAQQYRSRMASWFTATSQLEELSSFAAWNFNHPQFCTPELTT
                     AREIHGIEIGHPFIAEKSRISNDFSIGKNESIILITGSNMSGKSTFLRSIGINSILAH
                     CGTKVCARSFVFHPLQLLTSLRQSDSLHENVSLFHAELLRLKHIQNRISENGFSLVLI
                     DEMLRGTNSEDKLNGSRQLILRLIKTNCIGCIATHDLELGELETSHPGKISNYCFEST
                     IEGENLSFDYRLQKGIARNKNASFLLKKMNIIDGAP"
     gene            2092..>3826
                     /gene="purL"
                     /locus_tag="PK610_10065"
     CDS             2092..>3826
                     /gene="purL"
                     /locus_tag="PK610_10065"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HRJ36323.1"
                     /translation="MSMIPVGEPATLEQAKQLGLLPEEFEKIKEFLGRTPNFTELSVY
                     SVMWSEHCSYKNSIVWLKTLPKDGPHMLAKAGEENAGLVDIGNGLACAFKIESHNHPS
                     AIEPYQGAATGVGGINRDIFTMGARPVAQLNSLRFGNIDDARTKWLVKGVSKGIGDYG
                     NAFGIPIVGGEVFFDESFSTNPLVNAFSAGIMKVGEMISATSDGPGNPVFIVGSSTGK
                     DGIHGAAFASKDISEDSAKDLPSVQVGDPFMEKLLLEATMELKYTNCIVGMQDMGAAG
                     IACSTSEMSAKKGLGMKIDLSKVPARQEGMKPFEFLLSESQERMLIVVEKGKESEVQR
                     IFDKWDLNCAQIGEVTDTGWLEYYFEGELVGYVPADSLVLGGGAPVYKREWSEPAYFA
                     ETKKFSIDSLSEPSADEIKKYALQLLAQPNIASKRWIYEQYDSMVGTINMGTNKPSDA
                     GIVNLKGTDAALAMSVDCNSRYVFADPETGCAIAVSEAARNITCSGGEPSAITNCLNF
                     GNPYNPEVYWQFVGSIKGMGKACLKFGTPVTGGNVSFYNQSASVGKAGGNVSNAVFPT
                     PTIGMIGVVREK"
BASE COUNT         1026 a          749 c          867 g         1184 t
ORIGIN      
        1 caccgttcac tatcatggtg cgccatcaat aatattcatc ttttttaaca gaaaacttgc
       61 gtttttgttt cgggcaattc ctttttggag gcggtaatca aagctaaggt tttctccttc
      121 aatggtactt tcgaaacaat agttactgat ttttcccggg tgtgatgttt ctagttctcc
      181 cagttccaaa tcgtgtgtgg cgatgcatcc gatgcagttt gtttttatca ggcgaagaat
      241 caattgccgg ctgccgttta gtttgtcttc actgttggtt cctcttaaca tttcatcaat
      301 aagtaccagc gaaaacccat tttcactaat gcggttttga atgtgcttta agcgtaacaa
      361 ttctgcatga aacaatgaaa cattttcatg caacgaatcg ctttgacgta aagaggtaag
      421 cagttgcagg ggatgaaaaa caaatgaacg tgcacatact tttgttccgc aatgtgcaag
      481 aatgctattt ataccaatac tccggaggaa tgtacttttt cccgacatat tggatcctgt
      541 aatcagaatt atgctttcat tttttccaat tgaaaagtcg ttggaaatcc ttgatttttc
      601 ggcaataaat gggtgtccta tttctattcc gtgaatttct cgcgctgtgg tcaattcagg
      661 agtgcaaaac tgtggatgat taaaattcca ggcagcaaac gaactcaact cttccaattg
      721 agaagttgcg gtaaaccagc tggccattct tgacctgtat tgttgagccc attcgtgaag
      781 ccgggttgca caaaaaagat cgtatgcaat ggttgcgttg agcagcacga ataccaatgg
      841 gtttaagcgt cgatcaaatc gctcgctgat ttgagtaagt tctctgaatg ctttttcagc
      901 ttccttaaga tcgttttgaa ttgtaattgt tgctgacgat tcaaacttct cctccgaaat
      961 aagtttccat acctgtgcgt aggtcttcaa ttgatctttt agtccatcca atcctgcatg
     1021 agcctcttgg agctgtcgga ttaaaaccag tgctaccgaa agcgagatgc ctgatgccca
     1081 taacccgtaa tttgtttctc cggtaaagta ggttcccaac catcccgcag ttacaagaag
     1141 cggtaaggct attcgcagga tgtacaagag tttaatattt cctgttggag aatagttttc
     1201 cgaccactga tagagtgcgc tctcggggga cttgtcctca tcatttttga gcatcagtgc
     1261 aagaagcaat tcccggaatt ctgttttttc agccagttcc tctattgcag attgccgatc
     1321 ttttatttgt tgattttcaa tgggtggttc ggtcagaata gttgcaagtt tagcccttcc
     1381 tcccggtgaa tagcatcggg aaatacgatg gaaaagtgat tgctcaccaa atacatccag
     1441 atcatgcgca tagctggatg cttcttcatg cgcgctgcca ttgttcagca tggatgcttt
     1501 tccttcgaga agaactattt cgttagcaac aactttttcc cttcgcttga gaagatcttt
     1561 atcattactc agtttaatat gtttgatgac tgcaattatg aacgcaataa acagaaacgc
     1621 agatgtggca acaaaaaggg agtcgctgtt ttttattcca taccaaccca aagcaagcgc
     1681 acaactaaat agcaacagac gtaaaaacgt aattctgttt attttttttt cagtttcacc
     1741 aatttcctga tcgagtattt gttttcggtt attgtagtag ttagaaatgg tattcatgtt
     1801 gatctttatt gttgctgcca ttatcccttc attgaaatta gattactcaa ggtagaattt
     1861 ttgtgttttt gctcaatgaa gtaagtggtt tgcaaaacta agccatttga tagtatactc
     1921 tttgtaggaa ttttagttgt ttgaatttga gacaattgag attgagacct gttttttttc
     1981 aggttttgta gaattaatcg cctgttactg acaatctccc cgaatatgtt taaaactcct
     2041 ctcttttcaa gcccattttc actataaatt tgccgcgtaa acaacaccat catgagtatg
     2101 attcccgttg gcgaacccgc cacacttgag caggcaaaac agcttggact tcttcccgaa
     2161 gaatttgaaa agatcaaaga atttctgggc cgcactccca acttcactga gctttcagtg
     2221 tattcggtaa tgtggagcga gcactgttcg tataaaaatt caattgtttg gctcaagacc
     2281 ttgcctaagg atggtccgca catgctggct aaggcaggtg aggaaaatgc gggattggtg
     2341 gatatcggaa acggattggc ttgtgcgttt aaaatcgaat cgcacaatca cccttctgca
     2401 atcgaacctt atcaaggtgc agctaccggt gttggtggta tcaatcgcga tattttcaca
     2461 atgggtgctc gtcctgttgc tcagttaaat tcacttcgat tcggaaatat tgatgatgcc
     2521 cgaaccaaat ggctcgttaa gggggtctcc aagggaatcg gtgattatgg taatgcattc
     2581 ggtattccca ttgttggtgg tgaagtcttt tttgatgaaa gtttcagcac taatccgctt
     2641 gtaaatgctt tctctgcggg aatcatgaaa gtgggagaga tgatttccgc tacgtctgat
     2701 ggtccgggaa atccagtttt catagttggt tcttcaaccg gtaaagacgg gattcacggt
     2761 gctgcatttg catcgaaaga tatttcagag gattcagcaa aagatctccc atcggttcag
     2821 gttggggatc cgttcatgga aaaacttctg ctggaggcta caatggaatt gaaatatacc
     2881 aattgtattg ttggtatgca ggatatgggt gctgcaggaa ttgcctgttc taccagtgaa
     2941 atgtcggcga aaaaaggatt gggaatgaaa atcgatcttt ccaaagttcc ggcgcgtcag
     3001 gaaggaatga agcctttcga attcctcctt tcagaatcgc aggaacgcat gctgatcgta
     3061 gtggaaaaag gaaaagaatc ggaagtacag cgcatttttg ataaatggga tctgaattgt
     3121 gcgcaaattg gagaagtcac cgatacagga tggttggaat attattttga aggggagctt
     3181 gtggggtatg ttcctgcaga ttctcttgta ttgggtggag gcgctccggt ttataagcgc
     3241 gagtggtcgg agccggctta ttttgcagag acaaaaaagt tttcgatcga ttccttgtcg
     3301 gagccgtctg cagatgagat caaaaaatat gcactgcaac ttttggctca gcccaatatt
     3361 gcatccaaac gttggatcta cgaacaatac gattcgatgg tgggaaccat taatatggga
     3421 acaaacaaac catcggatgc aggaattgta aacctgaaag gcaccgatgc tgcattggct
     3481 atgtcggtcg attgcaattc acgttatgta tttgcagatc ctgaaaccgg atgtgcaatt
     3541 gcagtttctg aagcagcgcg caacatcact tgttcgggag gagaaccttc ggctattacc
     3601 aattgcctca actttggaaa tccttacaat ccggaagtgt actggcagtt tgttggttca
     3661 attaaaggta tgggaaaagc ctgccttaaa tttggtacgc ctgtaacagg aggaaatgta
     3721 agtttctaca atcaatctgc ttcggtagga aaagctggtg gaaatgtttc caatgctgtt
     3781 tttccgacgc caacaattgg aatgattggt gttgttcgtg agaagg
//
LOCUS       DAOMYG010000313         3823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8541_length_3823_cov_8.411359, whole genome shotgun sequence.
ACCESSION   DAOMYG010000313 DAOMYG010000000
VERSION     DAOMYG010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8541_length_3823_cov_8.411359"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..951)
                     /locus_tag="PK610_10070"
     CDS             complement(82..951)
                     /locus_tag="PK610_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HRJ36324.1"
                     /translation="MQILDGKKTSEEIKLEIKAEVDEIRRKGGKIPHLAAILVGSNGA
                     SMTYVNSKIKDCEEVGFKSSLIKLSDTVSEKELLRKIRELNADEDVDGFIVQLPLPQH
                     IEEKKVIKTIKPEKDVDGFHPENVGKMALNLPGFLPATPYGIIQLLQRYNVDTTGKHC
                     VVVGRSHIVGLPMSILMGRNAYPGNCTVTLTHSRTVNFKEICRSADILIVALGKPNHI
                     TADMVKDGVVIVDVGITRVPDTSKKAGYRIVGDVKFDEVAPKCLFITPVPGGVGPMTR
                     ASLLKNTLLASKR"
     gene            complement(1083..2273)
                     /gene="sucC"
                     /locus_tag="PK610_10075"
     CDS             complement(1083..2273)
                     /gene="sucC"
                     /locus_tag="PK610_10075"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRJ36325.1"
                     /translation="MNLHEYQGKEILSKYGVAIQRGIVAHTPEEAVAAGKQLESMTGT
                     KFYVVKAQIHAGGRGKGGGVKVAKSLDDLREKATNIIGMQLITPQTPPTGKKVHKVLI
                     AEDVYYPGPSEHKEFYMSILLDRAKGRHTVMYSPAGGMDIEAVAEETPHLIFKEEIDP
                     KVGLMPFQCRKIAFNLGCSGNAHKEMVKFVDALYKAFLGCDASMFEINPVFKTSDDKI
                     IAVDAKVTLDDNALFRHPAYAEMRDVTEEDPTEVEAASFDLNYVKLDGNVGCMVNGAG
                     LAMATMDIIKLSGGEPANFLDVGGTANAQRVENAFRIILKDENVKAILVNIFGGIVRC
                     DRVAQGIVDAYKNIGDIRVPIIVRLQGTNATEAKKLIDESGLKVHSVIQLKDAAAKVK
                     EVLA"
     gene            complement(2466..3407)
                     /locus_tag="PK610_10080"
     CDS             complement(2466..3407)
                     /locus_tag="PK610_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ36326.1"
                     /translation="MPFARMKMEIDKLSYSYKEGNHAVLNTVSFCIEDSFFSLGIAGR
                     SGSGKTTLLKAIYGLLNIRSGQVRFNGERVEGPDQHLIPGHPKMKLIAQDFALLPDHT
                     VEENIYTRLLAYTPEFRRAKADRLLQLCKIDHLRKEKPSALSGGQQQMLAISCALAEE
                     PELLLLDEPFSHLDKLTQSEIMQYLRELREELGFSFIFVSHDAPDVLEVCDRILIMEK
                     GKILEQGDPLSLYFSPKHEYTAALFGMYNKVGKQFIRPSSLRVTTEAHALFSGKVADS
                     GFRGESWWIKVRSGKKEMIYLSLNPPPAIGKLVHLKW"
     gene            complement(3389..3808)
                     /locus_tag="PK610_10085"
     CDS             complement(3389..3808)
                     /locus_tag="PK610_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36327.1"
                     /translation="MNKVLLAGFFMVLLLQSCFRSKECDPNKICDTNKPDSSWVNIQF
                     SATKYGIGVPFIIYNGDAEDELVVLRDTAFTDKLSYYLPISERYSVKAVYLINTTANT
                     TRVTAYDGGKLRLEKSWNCDERCYSAPDLNLDCRLLE"
BASE COUNT         1047 a          938 c          808 g         1030 t
ORIGIN      
        1 ccaataactc ctatctaatg actcctcttt aataactctt aactaataac taataccacc
       61 aaactcttcc cttccaacaa ctcacctctt cgacgccaac aaggtattct tgagcagaga
      121 agctctggtc attggtccta ccccacccgg caccggagta atgaacaaac attttggtgc
      181 cacttcatca aatttcacat cacctacaat gcggtaaccg gctttcttgc tggtatcagg
      241 tacgcgggta atacctacat ccacaatcac cacaccatcc ttcaccatat cggcggtaat
      301 atgattgggt tttccaaggg caacaattaa tatatctgca gagcggcaaa tctccttgaa
      361 gttaactgta cggctatgcg ttagtgtaac agtgcaattt cccggatagg cattgcgtcc
      421 catcaaaata ctcatgggca atcccacaat atgactgcgt cctaccacca cacaatgttt
      481 cccggtggta tcgacattgt agcgttgcaa aagctgaatg attccataag gcgtagcagg
      541 caaaaatccg ggtaggttca gcgccatttt ccccacattt tcggggtgga aaccatccac
      601 atctttttcg ggtttgatgg tcttgatcac ttttttctct tcgatatgtt gtggcaaagg
      661 caattgcaca atgaaaccat ccacatcttc gtcggcattc agttcacgga tcttacgcag
      721 caattctttt tcagaaacag tatcggagag tttgataagc gatgatttga aacccacctc
      781 ttcgcaatct ttgatcttgg aattcacata cgtcatgctc gccccattgc tccccaccag
      841 aatagcagcc aggtgtggaa tctttccacc cttgcgacgg atctcatcca cttcggcttt
      901 tatttccaat ttaatttctt cagaagtctt tttaccgtcg aggatttgca tgagaaacgt
      961 atttaaaaca aagataggaa aacccactaa ggcacaaaaa cacaatgaaa caccaacaaa
     1021 aaaaaggcgt ccttggacgc cttttctcta aaattatatc ttaatcactc ttcaaccaaa
     1081 aatcaagcaa gaacttcctt caccttcgca gcagcgtctt tcagctgaat tacggagtga
     1141 acttttagtc cgctttcgtc gatgagtttt ttcgcttcag tggcattggt cccctgcaaa
     1201 cgaacaatga taggaacacg gatatcgccg atgtttttgt aggcatccac aataccttgt
     1261 gcaacgcggt cgcaacgcac aattcctccg aagatgttaa cgaggattgc ttttacgttt
     1321 tcgtccttaa ggatgatacg gaaagcattt tcaacgcgct gtgcattggc agtacctccc
     1381 acgtcgagga agttagccgg ctcaccgcct gaaagtttaa tgatatccat ggtagccatt
     1441 gcgagtcctg ccccgtttac catacaaccc acgttaccgt cgagtttcac atagttcagg
     1501 tcgaaggaag cggcttctac ttcggtagga tcttcttcgg ttacatcgcg catttctgca
     1561 taagccgggt gacggaacaa tgcgttatcg tcgagcgtaa cttttgcatc tactgcaatg
     1621 attttatcat cggaagtttt gaaaacagga ttgatctcaa acatgctggc atcgcaaccg
     1681 aggaaagctt tgtagagcgc atcaacaaac ttcaccatct ctttgtgcgc attgcctgaa
     1741 caaccgaggt tgaaagcaat tttgcggcac tggaaaggca tcagtccaac tttcggatcg
     1801 atctcttcct tgaagatcag gtgcggcgtt tcttcagcaa ccgcttcgat atccatacca
     1861 ccggcaggag agtacatcac ggtgtgacga cccttagctc tgtcgaggag gatggacata
     1921 taaaattctt tgtgttcgga aggaccggga tagtacacat cttcagcgat cagcacttta
     1981 tgcacctttt tacctgttgg aggtgtttgc ggggtgatca gttgcatgcc gatgatgttg
     2041 gttgcctttt cacggagatc atccagactc ttggccactt tcactccacc gcctttacca
     2101 cgaccacctg cgtggatctg tgcttttaca acgtagaact tagttccggt catcgactca
     2161 agttgcttac cggcagccac cgcctcctca ggagtgtgag ccacaatgcc gcgctgaatg
     2221 gcgacaccgt acttggaaag gatctcttta ccttggtatt cgtgaagatt catgatgtgt
     2281 tttttaagtc tccaaatgta agcgttgcga gggaaaaagg gaaaaaaagt tttcctgtcc
     2341 ccggcaagac ctagcaagct gacttccagc aaaaaagatc cgaaggaaac acactgaatt
     2401 gtggaaaacc gatgttaata gccatgccat cgtactgctt aaagtgcgtc acatccggac
     2461 cggcatcacc atttgaggtg caccaatttt ccaatggcag gaggtggatt caggctcaga
     2521 tagatcattt ccttttttcc cgaacgaact ttgatccacc agctttcacc acgaaatccg
     2581 gaatcggcaa ccttacccga aaaaagtgcg tgcgcttcgg tagtgactct caaggaagag
     2641 ggacggatga attgtttacc gaccttattg tacatcccga atagtgcagc ggtgtattca
     2701 tgtttcggag aaaaataaag cgaaagcgga tcaccctgct ccagtatctt tcctttttcc
     2761 atgatgagaa tacggtcaca cacctcgagc acatcaggag catcgtgcga gacaaaaatg
     2821 aaagagaaac ccagttcctc ccgcaattcg cgcaggtatt gcatgatttc cgattgcgtg
     2881 agtttgtcga ggtgactgaa tggctcatcg agcaaaagca attccggttc ctcggccaat
     2941 gcacagctga tggcaagcat ttgttgctga ccaccactta gtgcagaggg tttttctttt
     3001 cgcaaatgat cgatcttaca caattgcagc aagcgatccg cctttgcacg acggaattca
     3061 ggggtgtagg ccagtaatcg ggtataaata ttttcttcaa cggtgtgatc gggcaaaagc
     3121 gcaaaatcct gagcaatcag tttcattttc ggatgcccgg gaatcagatg ctgatcggga
     3181 ccttccacac gttcgccatt gaaacgaact tgtccggacc ggatattcag cagaccatag
     3241 atcgctttaa gcaatgttgt ttttccacta ccgctgcgtc ctgcaattcc aagagaaaaa
     3301 aaactatctt ctatgcaaaa cgaaacagtg ttaagtacag cgtgatttcc ttctttgtag
     3361 gaataggaaa gtttatcgat ctccattttc attcgagcaa acggcaatcg agattgagat
     3421 caggagcgct gtaacaacgc tcatcgcagt tccatgattt ttcaagtcgc aatttaccac
     3481 catcgtaggc agtaaccctg gttgtattgg cggttgtatt gatcaaatac acagccttta
     3541 ccgaatagcg ttctgaaatg gggaggtaat acgaaagttt atctgtaaat gcagtatcac
     3601 gcaaaaccac aagttcatct tcggcatcgc cattgtaaat gataaaggga acaccaatcc
     3661 cgtattttgt ggcgctgaat tgaatattca cccaggaaga atccggttta ttggtatcgc
     3721 aaattttatt cggatcacac tccttactcc ggaaacagga ttgcaacaag agcaccataa
     3781 aaaaacctgc aagcagcact ttattcataa ttcagtgaga taa
//
LOCUS       DAOMYG010000314         3818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8563_length_3818_cov_8.490832, whole genome shotgun sequence.
ACCESSION   DAOMYG010000314 DAOMYG010000000
VERSION     DAOMYG010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8563_length_3818_cov_8.490832"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..830)
                     /locus_tag="PK610_10090"
     CDS             complement(39..830)
                     /locus_tag="PK610_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRJ36328.1"
                     /translation="MDKASLNYLSYMESAKSSMSEEHRELIEPLIKEPDLFLPIFHQM
                     ESVLFFIDYSSNTYPYISPNSKAVQGYSAELVKEEGPSNFLKRFDHSDLVVINEKLFP
                     ELLRMVKSLKGEELHHHKFCLNYRIILPDGKPRMLHQQSNILKVNNNHEPLLIAGTMT
                     HMKDGYKPGEMFLRFSRFHEDRGWELVEELLFKTNPGHKHPELSDAEKKVLVHIQKGL
                     TSKEAARELNLSPETINRHRKNIIKKLNASNMIEALQISRDNGLM"
     gene            complement(1068..>3818)
                     /gene="mfd"
                     /locus_tag="PK610_10095"
     CDS             complement(1068..>3818)
                     /gene="mfd"
                     /locus_tag="PK610_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRJ36329.1"
                     /translation="IEFFGDEVESIRSFDTITQLSKQTLNRINIIPNIQDKILRESRE
                     SFLDHIPNGTIIWISGKELVVDQMNKATERAQKAFAQLNSPLNHLSPEELYVSGSDLI
                     DHLQKFSVITLGRGGETEDVNEIIFELSPQPAFNKNFDLLAKNLSENTAKGYRNIILA
                     EQSRQVERLYSIFEDKQKEVKLSPIKMPISEGFIDHNNKLAIYTDHQVFDRYHRFRLK
                     EGFNKTREALTLKDLLSLQKGDFVTHIDHGIGQYSGLEKIEVNGKHQEAIRLIYKGGD
                     VLYVSIHSLHRISKYTGKEGTEPTLNKLGSNTWQTLKQKTKKKVKEIAYDLIQLYAKR
                     KSAPGFSYSPDNYLQTELEASFIYEDTPDQLKATQAVKQDMEQTFPMDRLVCGDVGFG
                     KTEIAMRAAFKAVNDSKQVAVLCPTTLLALQHFKNFRDRFKDFPCKVDYVNRFKSAKQ
                     RKETMEKLAKGEVDILIGTHAIVGKDVKFKDLGLLIIDEEQKFGVGVKDKLKLMRENV
                     DTLTLTATPIPRTLQFSLMGARDLSIISTPPPNRHPVQTDISTFNEEVIRDRITYEIS
                     RGGQVFFIHNRVQNIMDIAGLIQRICPDARICVGHGQMDGDKLEEVMVDFIEGEYDVL
                     IATAIIESGIDIPNANTIIINDAQNFGLSDLHQLRGRVGRSNRRAFCTLLTQPMHTLS
                     QEARKRLQAIEQFSELGSGFSIAMRDLDIRGAGNLLGGEQSGFISDIGYETYQKILDE
                     AIQELKENEFKELYQNEGIEPLEFVHECTIETDLEILLPDSYVNNVNERLILYRELDD
                     LKNEEELKKYRTMLSDRFGPIPPQIEELLETIRLRWKAKEMGFEKLLLKFNKLVGYFV
                     AKEDSPFYQSERFSKVLKYIQQHRDVKMEEKNGRLQLSFPSVNSIREALNKIEQILAT
                     PV"
BASE COUNT         1009 a          781 c          748 g         1280 t
ORIGIN      
        1 agaaaaatgg cgcacctata ctgctgccaa tacctgaatt acatcaatcc attgtcgcgg
       61 gaaatctgaa gtgcttcgat catgtttgaa gcattgagtt ttttgatgat gtttttacga
      121 tggcgattta ttgtttccgg actcagattt agttcacgtg cagcttcctt ggaagtaagg
      181 cctttctgaa tgtgtaccaa tacttttttt tcagcatcag agagttcagg atgtttgtgc
      241 cccggattgg ttttaaagag taattcctca actaattccc atccgcgatc ttcatgaaag
      301 cgactaaaac gcaaaaacat ttctccaggc ttgtaaccgt ctttcatatg ggtcatggtt
      361 ccggctatta ggagaggctc atgattgtta ttgaccttta aaatattact ttgctgatgc
      421 aacattctgg gttttccgtc gggaagaata atgcggtagt taagacagaa tttatggtgg
      481 tgcaattctt caccttttaa agacttcacc atcctaagta attcggggaa aagtttttcg
      541 ttgattacta ctaaatcaga atgatcaaaa cgctttaaga aattggaagg gccttcttct
      601 tttacaagtt cagcagagta tccctgtacg gccttagagt tgggactgat gtaaggataa
      661 gtgttgctgc tgtaatcaat aaaaaacaaa acactttcca tctgatggaa aatgggcaaa
      721 aacaaatccg gttctttaat cagtggctcg attaattcac ggtgttcctc gctcatggag
      781 ctctttgcag attccatgta actcaggtaa tttaggctgg ccttgtccat gatgtgtgtt
      841 ttaaattgtc taaaacaggt atgccagcag ctgagctgcc ctccattcgc caatggtaaa
      901 ctaaatataa gagaatgact tagataagca agtaggggaa ataccctatt tcggtaaatg
      961 aatatatttt cccgataaag tgaaaaaata ccggtattaa tgtgctaaaa aagaattgat
     1021 agggacaaca agcaatactg aatcggttta atttttttgt ttgttgtcta tacaggagtt
     1081 gcaagtattt gttcaatttt attcaaggct tcacgaattg aatttacaga gggaaaactt
     1141 aactgaagcc tgccgttttt ctcttccatt tttacatcac ggtgttgttg aatgtatttc
     1201 aatactttcg aaaaacgctc cgattgatag aatggcgaat cttcttttgc cacaaaatat
     1261 ccaacaagct tgttaaattt tagtagtaat ttctcgaatc ccatttcttt ggccttccac
     1321 cttaagcgaa tggtctctaa taattcttca atctgcggag gaataggacc aaatctgtcg
     1381 cttagcattg ttcggtattt tttaagttcc tcttcgtttt tcaggtcatc gagttccctg
     1441 tataggatga gtcgctcatt tacattgttc acataactat ccggcaacaa aatttcgaga
     1501 tctgtttcta tggtgcattc gtgaacaaat tccaaaggtt caatgccttc gttttgatag
     1561 agctctttga attcattctc tttcaattcc tgaatggctt cgtcaagaat tttctgatag
     1621 gtttcgtaac caatatccga aatgaatccg ctttgctccc cacctaataa attcccggcc
     1681 cctcgaatat caagatccct catggcaata ctgaatccac ttccgagttc agaaaattgt
     1741 tcgatggcct gcaaacgttt tcgcgcttcc tgactcagtg tatgcatggg ttgagttaac
     1801 aatgtgcaaa atgcccgcct gttgcttcgt cctactctac ctcttaactg atgcaaatcg
     1861 ctcagaccga aattctgtgc gtcattaatg ataattgtat tggcattcgg aatatcaatt
     1921 ccactttcaa taattgccgt tgcaatcagt acatcgtatt ctccttctat aaaatcgacc
     1981 attacctctt ccaacttgtc tccatccatc tgtccatgtc caacacaaat gcgggcatcc
     2041 ggacaaattc gctggatcaa gccggcaata tccattatgt tttgtacacg attgtgaatg
     2101 aaaaagacct gacctcctct cgaaatttca taggtaattc tgtcgcgaat cacttcttca
     2161 ttaaaagtgc tgatgtcggt ttgaaccgga tgcctgttgg gtggtggtgt actgataatg
     2221 ctgaggtcgc gtgcccccat cagggagaat tgcaatgttc gtggaattgg cgtggctgtg
     2281 agtgtaaggg tgtctacatt ctcgcgcatt aatttcaatt tatcttttac accaacgcca
     2341 aatttttgct cttcatcaat gatcagtaat ccgagatctt taaatttcac atctttccca
     2401 actatagcat gagtgccaat caaaatatcg acttcgcctt ttgccagttt ctccatcgtt
     2461 tcttttcgct gtttggctga tttaaaacga ttgacgtaat caaccttaca ggggaaatcc
     2521 ttgaagcgat ctctgaaatt tttaaaatgt tgtaaggcaa gtaaagttgt tggacacaaa
     2581 acggcaacct gtttcgaatc gtttactgct ttaaatgcag cacgcatggc tatttctgtt
     2641 tttccaaagc ctacatcacc gcacacaagt cgatccatag gaaaggtttg ttccatatcc
     2701 tgttttacag cttgtgttgc ctttaactga tccggagtgt cttcatagat aaatgaagct
     2761 tccaattctg tttgcaaata gttatcgggc gaataggaga atccgggtgc agattttctt
     2821 tttgcataaa gctgaatcag gtcgtatgca atttccttta cctttttctt tgttttttgt
     2881 ttgagggttt gccaggtgtt agaacccaat ttattcaaag tgggttcagt gccttcctta
     2941 ccggtatatt ttgatattcg gtgcagcgaa tggatactca catacagcac atcccctcct
     3001 ttgtagatga gtcgaatggc ttcctggtgt ttgccattga cttcaatttt ttccagtccg
     3061 gaatattgcc cgatcccgtg gtcgatatgg gtcacaaaat cccctttttg caaactcaga
     3121 agatctttaa gcgttaatgc ttcacgggtt ttgttgaatc cttcctttaa gcgaaaacgg
     3181 tggtagcggt caaatacctg atgatcggta tagatggcca gtttgttgtt gtgatcaata
     3241 aaaccttcgc tgataggcat cttgatcgga gataacttca cctctttttg cttgtcctca
     3301 aaaatggaat acaatcgttc aacctgacgc gactgctcgg ccagaatgat atttctgtaa
     3361 cctttggcag tattttcaga gagatttttt gccagcagat caaaattctt attgaaggcg
     3421 ggttgtggtg ataattcaaa aattatttcg ttcacatctt ccgtttctcc ccctcttccg
     3481 agtgttatta cactgaattt ttgaagatga tcaatcagat cgcttccgct tacatacagt
     3541 tcttcgggtg aaagatggtt aagcggactg tttaattgtg caaatgcttt ttgtgcacgc
     3601 tcagttgctt tgttcatctg atccactacc agttcctttc cgctgatcca gatgattgtg
     3661 ccgttcggta tgtgatcaag aaatgattca cgactttctc ttaaaatttt atcctggatg
     3721 tttggaatga tgttgatcct gtttaaggtt tgcttagaaa gctgtgtgat tgtatcaaaa
     3781 cttctgattg attctacttc atcaccgaaa aattcaat
//
LOCUS       DAOMYG010000315         3804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8606_length_3804_cov_8.636970, whole genome shotgun sequence.
ACCESSION   DAOMYG010000315 DAOMYG010000000
VERSION     DAOMYG010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8606_length_3804_cov_8.636970"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PK610_10100"
     CDS             complement(<1..767)
                     /locus_tag="PK610_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRJ36330.1"
                     /translation="MSSPKHKIFNDPVYGFITLPNNLALDIIDHPWFQRLRRIRQLGM
                     SHLVYPGALHTRFHHAMGAMHLMTQAIETLRSKGHSISEEEAEAVTIAILLHDIGHGP
                     FSHALENTIVEEVHHEEISLLFMERLNQEFKGKLDLAIKIFNNTYKKKFLHQLVSSQL
                     DMDRLDYLNRDSFFTGVSEGVVGSERIIKMLDVVKDHLVVEEKGIYSIEKFIIARRLM
                     YWQVYLHKTVVAAELMLINILKRAKELAMKGKKLWCTP"
     gene            863..2413
                     /locus_tag="PK610_10105"
     CDS             863..2413
                     /locus_tag="PK610_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HRJ36331.1"
                     /translation="MENINILWADDEIDLLKPHVMFLEQKGYQVTTVNNGADAVDKAR
                     SMEFSIVFLDENMPGLSGLETLERIKAIKPGVPVVMITKSEEESIMDSAIGGKIADYL
                     IKPVNPNQILLTLKKNLDSKRLVTEKTTMNYQQEFRQIGMQLGDLRTVEEWKDMFRKL
                     VYWEIELEKIADPGLHQILQMQKVEANNLFSRFVGDNYLDWVNGKADDIPLMSHQLLR
                     KKLLPDLEKDPVFLLVVDNLRFDQWKIIRPFLMEFFTEEEEDLYFSILPTATQYARNA
                     LFAGLMPSEIQKKYPSMWMNDDDEGSKNNFEKEFLEGLLKMQGKNVKFSYNKITNLNA
                     GKKVVDQFNNLLGNQLNVIVYNFVDMLSHARTDMEVIRELADNEVSYRSITRSWFEHS
                     PLFELCRKVAEKKLRLVITTDHGTVHVQEPSKIVGDRNTNTNLRYKQGKNLNYNSKDV
                     FEVKNPADAFLPKVNVSTTYVFAKDYKFFVYPNNYNQFVNFYQGTFQHGGISMEEMII
                     PYVSLKAR"
     gene            2453..>3804
                     /locus_tag="PK610_10110"
     CDS             2453..>3804
                     /locus_tag="PK610_10110"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36332.1"
                     /translation="MRLLFLFLFSFCSIILFSQTGPGGIGSLTDNALWLRANSGTSTT
                     TAGTAVQTWNDMSGNGNNVSQATVNQRPIFRTNIMNGYPAIQFDNVNTAGNNDFMEGA
                     DSPTLDNTNGLTIITVIRMTNLGDARSIVAKRTNVGVNQAYMFFLYTSNYLFIDVASN
                     DNRFSTNPTAYAINTNYMLSLLYDGSLSAPNRCKVYDAQALRVTSTESAATLPDYASP
                     LIIGATHVGDNRPFGGYIAEIIIFRRALNDTERVIVDNYLSSKYNIALSGNDYHSMDT
                     PANGDFDHEVAGIGRRIPAEFHNDAQGTSIVRINNPSALSNNEYLFWGHDNGTMTTNN
                     TVDVDGTIIQSRLNRIWRLTETGDVGTVTVSFDVSGFTSVTGSDLRLLIDRDGDGFFD
                     NDIAPQSGSFAGNIITFTNVDFTSGDRFTLGSINLNQTPLPVELLDFHASRNEGVVQL
                     G"
BASE COUNT         1118 a          735 c          842 g         1109 t
ORIGIN      
        1 ggtgtgcacc aaagtttctt tcctttcatg gccaattctt tggcacgctt gagaatattg
       61 atgagcatga gttctgccgc aactacggtt ttgtgcagat aaacctgcca atacatcagc
      121 ctgcgtgcaa taatgaactt ttcgatggag tatattcctt tttcttcaac tacaagatgg
      181 tcttttacca catccagcat tttgatgata cgctcgctgc caacaacgcc ctctgaaact
      241 ccggtaaaaa aactgtcgcg gttaaggtaa tccaaccgat ccatgtcgag ctgtgaacta
      301 acgagttgat gcaggaattt ctttttgtag gtgttattga aaattttgat ggcgagatcc
      361 agcttcccct taaattcctg attgagtcgt tccatgaaca ataaggaaat ctcctcatga
      421 tgcacttcct caacaattgt gttttccaag gcatgagaga atgggccgtg acctatatca
      481 tgcagcaaaa tcgcaattgt aaccgcctca gcttcttcct cacttatcga atgaccttta
      541 ctccttagtg tttcgatggc ctgtgtcatg aggtgcattg cgcccattgc atggtgaaaa
      601 cgggtgtgca aggcgccggg atataccaga tgcgacattc ccaactgacg aatacgtcgg
      661 agacgctgaa accagggatg atcgatgata tcaagcgcca gattattggg tagcgttatg
      721 aaaccgtaaa cagggtcgtt aaagatcttg tgcttgggag aagacatctt tttgttaatg
      781 atggttaaca aagataggga attggataat aaaccttact gcattgcgta tatagttatc
      841 ttagctactc aaatactttt tcatggaaaa tataaatatc ctctgggccg acgatgaaat
      901 tgacttgctt aaaccgcatg tcatgtttct tgagcaaaag ggctatcagg taactactgt
      961 aaacaatggt gctgatgccg tagataaagc ccgatcgatg gagttttcca ttgtatttct
     1021 ggatgaaaac atgcccgggc ttagcggact ggaaacgctt gagcgtatca aggccattaa
     1081 acccggagtt ccagttgtga tgatcaccaa gagcgaagaa gagtcaataa tggatagcgc
     1141 tatcggagga aaaattgccg attacctgat taagcctgtc aatccgaatc aaattctgct
     1201 aacgttaaaa aagaatcttg attcgaaaag attggtaacc gaaaaaacaa ccatgaatta
     1261 ccagcaggaa ttccgtcaga taggaatgca gctcggtgat ttgcgtacgg tggaggagtg
     1321 gaaagatatg tttcgcaagc tggtttattg ggagattgag ctggagaaaa tagcggatcc
     1381 cggattgcat cagattcttc aaatgcaaaa ggtggaagca aataatcttt tttcccgctt
     1441 tgtaggtgat aattacctgg attgggtcaa cggaaaggca gacgatattc ctttgatgtc
     1501 gcaccagctc ttgcgtaaaa aactattacc cgatcttgaa aaggatccgg tattcctctt
     1561 ggttgttgat aatctgcgtt tcgatcaatg gaaaattatt cgtccgtttt taatggagtt
     1621 cttcactgag gaagaagagg atctctattt ttcaattcta cccactgcga cgcaatatgc
     1681 acgtaacgca ttattcgcag gtttgatgcc ttcggagatt cagaagaagt atccttccat
     1741 gtggatgaac gatgatgatg aaggcagtaa aaacaatttt gagaaagaat ttcttgaagg
     1801 acttttaaaa atgcagggaa aaaatgtgaa attttcctat aataaaatca ccaatctgaa
     1861 tgcaggtaaa aaagtggtgg accaattcaa taatttgctc ggcaatcagc tcaatgttat
     1921 cgtttacaat tttgtggaca tgctttccca cgcacgaaca gatatggagg tgattcgtga
     1981 gcttgcggat aatgaggttt cgtacagaag tatcacccgc agctggttcg aacactctcc
     2041 gctttttgaa ttgtgcagaa aagtagcaga gaaaaaactc cgtttggtga taaccaccga
     2101 ccatggcacc gtgcacgtgc aggaacccag taaaattgta ggtgatcgaa atacaaatac
     2161 caatttgcgt tacaagcaag gtaaaaacct gaattataac agtaaagatg tttttgaggt
     2221 gaaaaatcct gcagatgcat ttttgccaaa ggtgaatgtg agtaccactt atgtttttgc
     2281 caaagactat aaattctttg tgtaccccaa caattacaat cagttcgtta atttctacca
     2341 gggcaccttt caacacggcg gcatctcgat ggaggagatg attattcctt atgtgagttt
     2401 aaaggcaaga tgagaattgg aaacacacac ataagtagct atttttgaaa ccatgaggct
     2461 actgtttctc ttcttgtttt ctttttgcag cattatatta ttcagccaga ccggtccggg
     2521 tgggattggt agtttaactg ataatgccct ttggttgcgt gctaattcgg gcacaagcac
     2581 tacaacagcg ggtactgccg tacaaacctg gaacgacatg tcgggcaatg ggaataacgt
     2641 gtctcaggca acggtgaacc agcgtccgat tttccgaacg aatatcatga atggttatcc
     2701 tgctattcaa tttgataatg tgaataccgc aggaaataac gattttatgg aaggggcaga
     2761 ttcacctact ctggataata caaatggact taccatcatt actgtaatac ggatgacaaa
     2821 tcttggtgat gcacgttcaa tcgtggcaaa gcgtaccaat gtcggcgtta atcaagccta
     2881 tatgtttttc ctgtatactt ccaattatct ttttattgat gttgccagta acgataatcg
     2941 cttttcaaca aacccaacgg catatgcaat aaatactaat tatatgctgt ctttgctata
     3001 tgatgggtct ttatccgcac caaatcgatg caaagtttat gatgcccaag cattaagagt
     3061 tacttccaca gaatctgcag caacattgcc cgattatgct tcgccattaa taattggcgc
     3121 aactcacgtt ggcgataatc gtccgtttgg tggatatata gctgaaatca tcatttttcg
     3181 acgcgcattg aatgatacgg agcgtgttat agttgataat tatctttctt ccaagtataa
     3241 tatcgccctc agcggaaacg attaccattc catggataca cctgcgaatg gggatttcga
     3301 tcatgaagtg gcgggaatcg gtcggaggat acctgcagag tttcataacg atgctcaggg
     3361 aacttccatt gtccgtataa ataatccatc tgcattaagc aacaatgaat acttgttttg
     3421 ggggcatgat aatggaacca tgactaccaa taacacagtt gatgtagatg gtacgatcat
     3481 acaatcgagg ttaaatcgta tttggagatt gacggaaacc ggtgatgtag gtacagttac
     3541 tgtttcgttc gatgtgagtg gttttacgtc ggttaccgga agtgatttac gattattgat
     3601 agaccgtgat ggtgatggtt tttttgataa tgatattgca ccacaaagtg gaagttttgc
     3661 aggtaatatc atcacattta caaatgtcga ttttacatct ggcgatcgct ttacacttgg
     3721 ttcaatcaat ttaaaccaaa cacctttgcc ggttgagctt ctcgattttc acgcaagtag
     3781 aaatgaagga gttgtacaat tggg
//
LOCUS       DAOMYG010000316         3789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8650_length_3789_cov_9.433583, whole genome shotgun sequence.
ACCESSION   DAOMYG010000316 DAOMYG010000000
VERSION     DAOMYG010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8650_length_3789_cov_9.433583"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="PK610_10115"
     CDS             complement(<1..148)
                     /locus_tag="PK610_10115"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaC family regulatory phosphoprotein"
                     /protein_id="HRJ36333.1"
                     /translation="MEDLHIEGTTKTPEIHFEASTGILEIKGRSIPEISVEFYQAAIN
                     WLKDY"
     gene            571..954
                     /locus_tag="PK610_10120"
     CDS             571..954
                     /locus_tag="PK610_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36334.1"
                     /translation="MRFYLSLLLFTFLGFQLSAQSVQTATFKDTKSLFAKEGKAYTVK
                     FQLSTFPSDEVRDAFVAYASSNNITSRFSPNKKGGANVILLFPENLANGSVLGKMLMQ
                     LGVVDVIVQSKGDSKSSVEEFIQTI"
     gene            957..>3789
                     /locus_tag="PK610_10125"
     CDS             957..>3789
                     /locus_tag="PK610_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRJ36335.1"
                     /translation="MKKLILLAGIFAFLGFQSASSQIVGVGHSSQASTTYTSTATNDT
                     IYFWCGAGALGTLTATPPSGVPGWNFIWQVFNPATNSWNPFTVQNGVPTSTINNLAGG
                     LYRVSIVDGTSTVVGCYRAWVSIVSSPTTVNVAPIPPGCGAVQLNGTINYGSATQLYN
                     PPADPFLVGPTTTITVCFTANHTYVSDLAFYLVGPTTCGSPSILLSPNPGAIGQGAIC
                     NGGNNVANLCFTTNPAGNLNVCTATVPLTGTYSSYGPANTPINWNGIYGCDATLGGWR
                     VQIYDCIGADVGALTNSTITFTGQTTCGQNSTVTYNSGAINSPITDNSCSAATASIFT
                     VPVSPASPIPYAGGYIWTANPAITIPNATTSLNPLVNPGPTQNTIFTLTLTGGSIPTC
                     NGGNNSDSEPYIYTPPTFPTITPVAPVCVNSGSFALTANIPGGTWAGPGIIDPVNGIF
                     DPSVAGVGTPTVTYIFTGPCGGGDSETITVTGLSNSTITPAGPFCTTDPIINLTAASP
                     GGTWSGTGITNPSTGAFNPALVSGTTTITYTISGACGSSDTETITVNTANPIINPVSP
                     LCVNGSPINLTANSTGTWSGNGITNPANGTFDPSVAGVGTHVITFTVASPCPGSTTVS
                     ITVNALPAANAGNNASFCAGGAGVQLNATGGVTYAWSPTTGLSNPNIANPTATPASTT
                     TYTVTVTGNNGCSTTSSVTVTVNPLPTVSLPANATFCAGGSTVLNVTGTATSFAWTPS
                     NGLSSTTSSNPTASPSANTTYTVTGTNANGCTATASVNVVVSSITATATATPPAGVEP
                     LDVVFTNTTVGGVMYAWNYGNGQSEITANTTTSATYPVDSTYFVTLIATNADGCVDTL
                     VLTIVVYDDFSLLIPNVFSPNGDGSNDEFRIVATGVVNMTMTIYNRWGKEVGMFNSPS
                     GSWDGKNNGKMSPEGTYFYIFKAEKSDGTP"
BASE COUNT         1036 a          848 c          748 g         1157 t
ORIGIN      
        1 tataatcttt tagccagttg atagcagcct gataaaattc aacagaaatt tcaggaatag
       61 aacgaccctt aatttccaga atacccgttg aagcctcaaa atggatttca ggggtttttg
      121 tcgttccttc tatgtgcaga tcttccatgg ttcagtgtga caccgacgaa gataaattga
      181 aaatgcttga ataatagctc tttggacttc gatttcgtcc agttcaagga attaaaggat
      241 aaaaacaggg acaaagccga ctgggggctt aaaatgatac aggatcgcct gtttttttta
      301 gcattgacta tttaaaccca tttagatttc tggattgcgc gtttctggtt tagaaaatcg
      361 ccctttaaag gtatttaata gcttgttaat caaataatta tttgatttta catttaagct
      421 tagccgcatt gatatgtttt ttgacctgaa tatcgatgat tcttcatcga aaacttatca
      481 ttggtcagat tgttaatgta aaaccaatta aaaattaaac agtacgcaac cttttgcgtt
      541 aattttggtc tttcaaaaaa cacatttgtt atgagattct atctttccct gcttcttttt
      601 acatttttag gttttcagct ttctgctcaa tcggtgcaga ctgcaacctt taaggatact
      661 aaatcgcttt ttgccaagga aggtaaagcc tacactgtaa agtttcaatt gagcactttt
      721 ccttctgatg aggttcgtga tgcttttgtt gcgtatgcat catccaataa tataacttcc
      781 aggtttagtc caaacaaaaa gggaggggca aacgtcattc tgcttttccc tgaaaacctc
      841 gcaaatggat ctgttctggg taaaatgctg atgcagcttg gagttgtcga cgttatcgtt
      901 cagtcgaaag gtgattcaaa atcttctgtt gaagaattca ttcaaaccat ttaagcatga
      961 aaaaattaat tctacttgcc ggtatttttg cttttctggg ttttcagtca gcaagctctc
     1021 aaattgttgg agttgggcat tcaagtcagg cttcaactac ctatacaagc acagcaacca
     1081 atgacacgat ttatttctgg tgtggtgcgg gtgcgcttgg tactttaaca gcaactcctc
     1141 cttcgggtgt tccgggttgg aattttattt ggcaggtttt taatcctgca actaattcct
     1201 ggaacccttt cactgttcaa aatggtgttc caacatctac cattaacaac cttgctggtg
     1261 gtttgtaccg tgtatcaatt gttgatggta cttccactgt agttggctgt tatcgtgcct
     1321 gggtttccat cgtttccagt ccaactactg ttaatgtagc gcctattcct cccggttgtg
     1381 gtgctgtaca gctaaatggt acaattaatt atggctctgc tacacaattg tacaatcctc
     1441 ctgcagaccc attccttgtt ggccctacta ctacaataac cgtttgcttt actgcaaatc
     1501 atacctatgt atcggatttg gcattttatc ttgttggtcc taccacttgt ggaagcccaa
     1561 gtattttgct ttctcccaat cctggtgcta tcggtcaggg agctatttgt aatggtggaa
     1621 acaatgttgc aaatctttgt tttacgacca atcctgcagg aaatttgaac gtttgtactg
     1681 ccactgttcc cttaacaggt acctattctt cctatggtcc ggcaaataca cctatcaact
     1741 ggaatggaat ttatggatgt gatgccaccc ttggcggatg gagagttcag atttatgatt
     1801 gtattggagc tgatgttgga gcgcttacca attcaacaat tacattcaca ggtcaaacta
     1861 catgtggtca aaactcaaca gtaacctaca attcaggtgc tattaactca ccaattactg
     1921 acaactcctg ttctgctgca accgcttcaa tctttacagt tccggtttct cccgcaagcc
     1981 caatccctta cgctggtggg tatatttgga ctgctaatcc tgccattaca attccgaatg
     2041 ctactacttc gctcaaccca ttggttaatc ccggtccaac acaaaacact attttcacat
     2101 taacgcttac aggaggatca attccaactt gtaatggcgg taataattcc gattcagaac
     2161 catacatcta tactccacca actttcccaa caatcacgcc tgttgcaccg gtttgtgtta
     2221 attccggatc ctttgcttta acggcgaata tcccaggagg aacctgggct ggtcccggta
     2281 tcatagatcc ggtaaacgga attttcgatc cttctgttgc cggtgttgga actccaaccg
     2341 tgacttatat ctttaccggt ccatgtggag gaggtgattc tgaaacgata actgtaacag
     2401 gtctttccaa ttcaactatt acacctgcgg gtccgttttg tacaaccgac ccaattatca
     2461 atttaacagc agcttcaccc ggcggaacct ggtcgggaac cggaataacg aatccttcaa
     2521 caggcgcatt taatcctgcc ctggtttcag gaacaacaac aattacctac accatttcag
     2581 gtgcatgtgg aagttcggat accgaaacga taaccgttaa cacggccaac ccgatcatta
     2641 acccggtttc tccattgtgt gtgaatggat cgcctattaa cttaactgct aacagtacgg
     2701 gaacctggtc cgggaatgga ataaccaatc ctgcaaatgg tacgtttgat ccatctgttg
     2761 caggtgtagg tactcatgta attacattca cagtggcatc tccttgtccg ggttcaacaa
     2821 ccgtcagtat cacagttaat gccttaccgg ctgccaatgc aggaaataat gcatcatttt
     2881 gtgcaggagg tgcaggagtt caattaaatg caaccggtgg tgttacctat gcatggtcac
     2941 caacaaccgg gttgagcaac cctaatattg ccaatccaac agcaacgccg gcatctacaa
     3001 caacttatac tgttacagta acaggaaaca atggttgttc gacgacctca agtgtaactg
     3061 taacagtaaa tccattgcct acggttagcc ttcctgcaaa tgcaactttt tgtgcaggcg
     3121 gatcaactgt tctcaatgta acaggaacag caacaagttt tgcatggaca ccttctaatg
     3181 gattaagttc cactacaagt tccaatccaa cggcctctcc ttctgcaaat acgacctaca
     3241 cagttactgg tacaaatgct aacggatgta ccgcaactgc aagcgtaaac gtagttgttt
     3301 catccataac agcaacagca acagctactc ctcctgcagg agtagaacca ttggacgtgg
     3361 tatttaccaa tacaactgtt ggtggtgtga tgtatgcctg gaattatggc aatggtcagt
     3421 ctgaaattac tgccaatacc acaacttctg cgacataccc tgttgacagt acttactttg
     3481 ttacgcttat tgctaccaac gccgatggtt gcgttgatac tcttgttctg actattgttg
     3541 tctatgatga tttttcactt ctaattccaa acgtgttctc accgaacgga gatggatcga
     3601 atgacgaatt cagaatagtt gcaaccggtg ttgtcaatat gacaatgacc atctataatc
     3661 gttggggtaa ggaagtagga atgttcaatt caccaagcgg atcctgggat ggcaaaaaca
     3721 acggaaagat gtcaccagaa ggaacctatt tctacatttt caaagcagag aaatctgatg
     3781 gtacaccca
//
LOCUS       DAOMYG010000317         3776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8684_length_3776_cov_8.476216, whole genome shotgun sequence.
ACCESSION   DAOMYG010000317 DAOMYG010000000
VERSION     DAOMYG010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8684_length_3776_cov_8.476216"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PK610_10130"
     CDS             <1..650
                     /locus_tag="PK610_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36336.1"
                     /translation="TEYYRQIINEESNFIVTGFDFRYYQKIHRDFIFAGRAAGSASFG
                     DRRLLYYLGGVDNWIAPRFDRTIQVAPNQNYGFQTIATPVRGFFQNARNGSNMGVINS
                     ELRMPLIKYFAEKPLKSDFLENFQVVGFFDVGSAWTGPNPYSDDNAFNTIQYSSQGNP
                     VIITIKNQREPIIYGYGWGLRSKLFGYFLRFDWAWGVDDGLRLAPVRYFSLTLDF"
     gene            656..1051
                     /locus_tag="PK610_10135"
     CDS             656..1051
                     /locus_tag="PK610_10135"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRJ36337.1"
                     /translation="MDASTILILSIIGFSAGLLGGFVGVGGGIIIVPALIYFLGLSQH
                     MAIGTSVAVMLPPIGIGAALVYYNNGDINTPYAIVIAIAFILGGYLGGLASQSLKESV
                     HVIKLLFGLVMLYSAGKMIFSALKVFLEK"
     gene            1048..2229
                     /locus_tag="PK610_10140"
     CDS             1048..2229
                     /locus_tag="PK610_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRJ36338.1"
                     /translation="MISAGEIRKLSEKYFSKVIAIRRHLHSHPELSFQEYATSEYVCS
                     ILRDLKIEFKSGIAGTGIIAHINGKNPESGLKAFRADLDALPIHEKNEVEYKSCNTGV
                     MHACGHDVHTACLLGAAMILQETRDKWKGTIRMIFQPGEEQLPGGASLMINEGALENP
                     RPEFIIGQHVFPELEAGKVGFRKGMYMASTDELFLTVKGKGGHAALPSQYTNPILIAA
                     SILLRLQNEFMHPEIQHPAPTVLAFGKIEGAGATNVIPSEVKLEGTFRTMNETWRTIA
                     HERMMEIASSVAAEFGGSVDFEIKKGYPFLVNDLSTTQMMIDSAREYLGAENVVELDL
                     RMTAEDFAWYSQVLPSCFYRLGTRNESKGIISPVHTATFDVDENAMLTGMGLLAFAAS
                     R"
     gene            complement(2230..3540)
                     /gene="der"
                     /locus_tag="PK610_10145"
     CDS             complement(2230..3540)
                     /gene="der"
                     /locus_tag="PK610_10145"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019668810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRJ36339.1"
                     /translation="MSNILAIVGRPNVGKSTLFNRLTESRDAIVDPTEGVTRDRHYGK
                     SFWNGHEFSVIDTGGYIVGSEDVFEEEIRKQVKLAIEEATVILFMVDVMAGVTDMDRE
                     VANLLRRSKKRVLLVANKIDTPDKAPFAAEFYSFGLGEIWNISAQNGSGTGELLDEVT
                     KDFINKSDDEEEKTRMPRFAIVGKPNVGKSSLLNALVGQDRNIVTSISGTTRDSILTR
                     YNAFGFEFDLIDTAGIRKRAKVHEDIEFYSVLRSIRTIEESDVCLFMIDAKEGIQKQD
                     LHIYYMIESNKKGVVVLVNKWDLVEKDTKTLKAYEDNLRQALAPFNDVPILFISALTK
                     QRIHKALEEAMRVYQIRSSKIPTSQLNEILLPILEQSPPPSYKSKYIRIKYVTQLPTA
                     VPSFAFFCNLPQYIKDSYKRFIENQLRKHFDFNGVPIRIFFRKK"
     gene            complement(3545..>3776)
                     /locus_tag="PK610_10150"
     CDS             complement(3545..>3776)
                     /locus_tag="PK610_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRJ36340.1"
                     /translation="EEPEITRISAVIYVSRDSQKGIIIGHQGKMIKKLGTLARKEMEE
                     MLNKKVFLELFVKVMKNWRDDERHLKNFGYTN"
BASE COUNT         1040 a          719 c          806 g         1211 t
ORIGIN      
        1 ggactgagta ttacaggcag atcataaacg aagaatcaaa tttcatagtt accggatttg
       61 attttaggta ctaccaaaaa atccatcgtg attttatttt cgccggccgt gcagcaggga
      121 gtgcttcttt tggggacaga cgattgttgt attatttagg cggtgtagat aactggatag
      181 caccacgatt tgatcgtact attcaggttg ctcccaatca gaattatgga ttccagacta
      241 ttgccactcc tgttcgtggg ttttttcaga acgcacgaaa tggttccaat atgggagtaa
      301 tcaattcaga gttacggatg ccacttatta aatattttgc tgagaagcca ttaaaatccg
      361 acttcttgga gaatttccag gttgtaggat tttttgacgt tggttccgca tggacagggc
      421 ccaatccgta ttcagatgac aatgcattta acaccattca atattccagt caggggaatc
      481 cggttatcat cacaattaaa aatcaacgag agccaatcat ttacggttat ggctggggat
      541 tgcgatcaaa attattcggc tatttcctgc gttttgattg ggcatggggt gttgatgatg
      601 gtttgcgttt agctcctgtt cgttacttct ccctcacgct tgatttctaa tgcgaatgga
      661 tgcttcaact attctaatct tatcaataat cggattttct gccggtttac ttggaggatt
      721 tgttggagtt ggcgggggaa taataattgt acctgcgctt atctattttc ttggccttag
      781 tcagcatatg gcaattggga cttctgttgc tgttatgctt cctccaatag ggattggtgc
      841 tgctcttgta tattataata atggggatat caatacgcct tatgctattg ttattgcaat
      901 tgcatttatc ctgggtggat atcttggtgg cttggcttct caatcgctaa aagagtcggt
      961 acatgtgatt aaacttttgt ttggattagt catgttgtat tctgctggaa aaatgatttt
     1021 ttcagcgctt aaagtttttt tggaaaaatg atttctgcag gagagataag aaaactttca
     1081 gaaaaatatt tctcaaaggt tattgctatt cgaaggcact tgcattcaca tcctgaattg
     1141 tcgtttcagg agtatgcaac atcagaatat gtttgttcca tacttcgaga tctaaagatt
     1201 gaatttaaat ccggaattgc cggaaccgga ataattgccc acatcaatgg aaagaatccg
     1261 gaatcgggcc taaaagcttt tcgtgctgat ttggatgcct tgcctatcca tgaaaaaaat
     1321 gaggtggaat acaagtcgtg caatacaggt gttatgcatg catgtggtca cgatgtgcat
     1381 actgcctgtt tgttaggtgc agccatgatt ttgcaggaga cccgggataa atggaaaggc
     1441 accatccgaa tgatcttcca accgggcgaa gagcaattac ccggtggggc ttctttaatg
     1501 atcaatgaag gcgcattaga aaatcctcgg ccggaattta ttattggtca gcatgttttt
     1561 ccggagctgg aagcaggtaa agttggtttc agaaagggaa tgtacatggc atctaccgac
     1621 gaattgtttt tgacagttaa aggtaaagga ggacatgcag ctttaccttc tcaatacacg
     1681 aatcctattc tgattgccgc atcaattttg ttgcgcttgc aaaatgaatt catgcatccg
     1741 gaaattcaac atccagcccc aacagttttg gcctttggga aaattgaagg tgccggagca
     1801 acaaatgtta ttccttcaga agtaaaacta gaaggtacgt tcagaaccat gaatgaaaca
     1861 tggagaacaa ttgctcatga acgtatgatg gaaattgcat cttcagttgc tgctgagttt
     1921 ggtggttctg tagattttga aattaaaaag ggatatccat ttctggtaaa cgatttatcg
     1981 accacccaaa tgatgattga ttcggcacgt gaatatttag gagcagaaaa tgttgttgag
     2041 cttgatttgc gaatgactgc cgaagatttc gcttggtact cacaggtttt gccttcttgt
     2101 ttttacagat tgggaacccg taacgaatca aaaggaatta tctcgcctgt acacaccgca
     2161 acttttgatg tagatgaaaa cgcgatgttg accggtatgg gattgttggc ttttgctgcc
     2221 agccgctgat tatttttttc ggaagaaaat cctgattgga acaccattaa agtcgaagtg
     2281 ttttctcaat tgattttcaa taaaacgctt gtaagaatcc ttaatgtatt gcggcagatt
     2341 acaaaagaat gcaaaacttg gaacagctgt tggtaactgt gttacgtatt ttatcctgat
     2401 gtatttgctt ttatacgatg gcggaggact ctgctcaaga ataggcagca gaatttcgtt
     2461 aagctgtgac gttggaattt tactgctgcg tatttggtaa acacgcatcg cctcttcaag
     2521 agccttatgt atgcgctgtt tggttagcgc agaaataaac agaatgggga catcattaaa
     2581 aggcgctagg gcctgacgca gattgtcttc gtaagcttta agtgttttgg tgtctttttc
     2641 aacaagatcc cacttattta ccagcacaac aactcctttt ttgttgcttt caatcatgta
     2701 gtaaatgtgg aggtcttgtt tttgaatgcc ttcttttgca tctatcataa aaagacaaac
     2761 atccgattct tcaattgtac ggattgaacg taatacagaa taaaactcga tatcctcgtg
     2821 aacttttgca cgctttcgaa tacctgcagt atcaataagg tcgaattcga atccgaaagc
     2881 attgtagcgt gttaaaatag aatcgcgggt agttcctgag atggaagtaa caatgtttct
     2941 gtcctgacca accaacgcat ttaacaatga tgattttcca acattaggtt ttccaacaat
     3001 tgcaaatcga ggcattcggg ttttctcttc ctcatcatca gatttgttga tgaaatcttt
     3061 tgtgacttca tcaagcagtt caccggttcc ggatccgttt tgtgcagaaa tattccagat
     3121 ttcaccgaga ccaaaactgt agaattctgc tgcaaaaggg gctttatccg gagtgtcaat
     3181 tttgtttgca accaacaata ctcttttttt tgaacggcga agaaggttgg caacttcacg
     3241 atccatgtca gtgacaccgg ccattacatc aaccataaat aaaattacag ttgcctcttc
     3301 aatagcaagc ttaacttgct ttcgaatttc ttcttcaaat acatcttcac taccaacaat
     3361 atatccaccg gtgtcgatca cagaaaattc atggccattc caaaatgatt ttccataatg
     3421 gcgatcacgt gtaacacctt ctgttggatc aacgatggca tctctgctct ctgtaaggcg
     3481 gttaaataag gtagatttcc caacattggg ccgacctact atggcgagga tgtttgacat
     3541 gcgattaatt agtgtatccg aagtttttta aatgacgttc atcgtcccgc cagtttttca
     3601 tcactttcac aaagagttcc agaaatactt ttttattaag catttcttcc atttcttttc
     3661 gtgccaaagt gccgagtttt ttaatcattt ttccctggtg accaataatg atcccctttt
     3721 gagagtcgcg tgaaacgtaa atcacagctg agatccgcgt tatttcaggt tcttct
//
LOCUS       DAOMYG010000318         3758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8744_length_3758_cov_9.182825, whole genome shotgun sequence.
ACCESSION   DAOMYG010000318 DAOMYG010000000
VERSION     DAOMYG010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8744_length_3758_cov_9.182825"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PK610_10155"
     CDS             <1..327
                     /locus_tag="PK610_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36341.1"
                     /translation="RTIAFRDPRTRLWQRFYFEGMQLNTIGFDHQGQLYAAAGFRYMF
                     YCDGAAEQLQSAGLYRFVPGSNGAEWKKVENPVNPKILSITTWPGRGLLLGTSGSGLV
                     GVSISK"
     gene            369..614
                     /locus_tag="PK610_10160"
     CDS             369..614
                     /locus_tag="PK610_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36342.1"
                     /translation="MNLDEFRELSLSHRADLVQAEGKFIASRSAQTFHVDLYRVRNFY
                     VEIWNNTIHGILMQIDGFEDDQFLKPYLDDINVKDLF"
     gene            complement(635..1918)
                     /locus_tag="PK610_10165"
     CDS             complement(635..1918)
                     /locus_tag="PK610_10165"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRJ36343.1"
                     /translation="MISCISLFILSYPFPQIFPIALASVLLAFSLLVLDVFLLFLSKS
                     SVHAKRLLPPLLTLGDEVEIRIRVASRMNTSVKIEITDETPEQLQLRDLRIYTELGPG
                     DSEIVRYTIRPTERGAHRFGDIRIFASGIIGLVQRRITIPAEEEMAVFPSVLQMRQYE
                     LKVFSKLTLTEGIKRVRRLGQSTEFEQIKNYVSGDDYRNINWKASARRSELMVNQYED
                     EKAQQVYCLIDKSRSMRMAFEGMTLLDYAINSSLVISNIAMRKSDRAGLMTFSDKLGT
                     RLPAERNASQLKRIQEALYRQKTRFNEANFEMLYFGIRNYIKGRSLLLLFTNFESSYA
                     VQRALPLLRRINQQHLLVVVFFENTELKEVTQMETPDLRAVYTKTVAQHLAGEKQLIA
                     QELSRHAIQTILTTPEKLSVDTINKYLELKSRGMI"
     gene            complement(1966..2970)
                     /locus_tag="PK610_10170"
     CDS             complement(1966..2970)
                     /locus_tag="PK610_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRJ36344.1"
                     /translation="MEEQNTPLGSFSFDNGAGARLAQLRENIQQARTEIRKYVIGQDE
                     MIELLLVGILSNGHILLEGVPGIAKTLTAKVFSKTLSAHFARIQFTPDMMPSDIIGTS
                     VYNQKDGNFQFKRGPVFSNIILIDEINRAPAKTQAALFEVMEERQITYDGVTHSMEFP
                     FLVLATQNPIEQEGTYRLPEAQLDRFLFRVKLHYPTLTEEKEILLRYKDEIKSPDLSE
                     IKAIFTVAVLKEIQETIRTVKVEDQLIKYIADIIHSTREHAKLYLGASPRASLALLKS
                     AKTLAAIRGRDFIIPDDVQFLANHVLNHRIVLSPEAEMEGLRTEDIIQDILQKIEVPR
                     "
     gene            complement(2971..>3758)
                     /locus_tag="PK610_10175"
     CDS             complement(2971..>3758)
                     /locus_tag="PK610_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36345.1"
                     /translation="SLYFDTDRGELDSASLENAIFENEFVGEATELQVLKDEQRFVYV
                     RINYGEGAFFIHFIPYAFTNISLIREENVEHFEKILSYLPPVDLMYVDEGFQPRQHTE
                     GGDNEAPTGTSRTSPLQFILSVQSLSWAYYMTLITLLLFILFRIKRRQKPIPARGKIE
                     NSTLNFAATLSELYFQQGKHQYLVIQKQRLLMNFIRERYYLKSRTADEAFIRSLAVKS
                     GISQEKLNELFKSLKNGAEQIRLSDEQFIAIHQLTEYFYKNCK"
BASE COUNT          995 a          788 c          823 g         1152 t
ORIGIN      
        1 cgtacaattg cttttcgtga tcctcgaacc cgtctttggc agcggtttta ttttgagggt
       61 atgcaattga atacaattgg ttttgaccat caggggcagc tttatgctgc agcaggattt
      121 cgttacatgt tttattgcga tggcgctgct gaacaattgc aatctgccgg attgtaccgc
      181 tttgtaccgg gatccaatgg cgcagaatgg aaaaaggtcg agaatccggt taaccctaaa
      241 attctcagca tcaccacctg gccgggtagg ggactgctac tgggcacttc gggttccgga
      301 ttggttgggg taagcatctc gaagtgaaaa caagcgataa gcaatgcttt atatttttaa
      361 attgcagtat gaatcttgat gagttcagag aattatcctt aagtcatcgt gccgatctcg
      421 ttcaggcgga gggaaaattc attgcgtcgc gctctgcaca aacctttcat gtggatttat
      481 acagagtccg gaatttctat gtcgaaattt ggaacaatac cattcacgga attctgatgc
      541 aaatcgatgg cttcgaagat gatcaatttc tgaagccgta cctcgatgat atcaatgtaa
      601 aagatttatt ctgatcacac tgaggctttg ctcatcaaat cattccccgt gatttcaatt
      661 ccaggtattt gttaatggtg tcaacactga gtttttccgg agtagtgaga atggtctgga
      721 tggcatgtcg cgacaattcc tgagctataa gttgtttttc acctgctaaa tgttgcgcca
      781 cagttttggt gtacaccgca cgtaggtcgg gagtttccat ttgtgtgact tccttcagtt
      841 ctgtattctc aaaaaataca accactagca aatgctgctg attgatcctt ctcagcaatg
      901 gaagtgcacg ctgaacggca taggaacttt caaaattggt aaagagcaat aacaaacttc
      961 ttcctttgat ataattgcga ataccgaaat aaagcatttc aaaattcgct tcgttaaacc
     1021 gggttttctg gcggtacaat gcttcctgaa ttctttttaa ttgcgaagca ttacgttccg
     1081 caggtaatcg agttccaagc ttatcactaa aagtcattag tccggcccgg tctgattttc
     1141 tcatggcgat attagagatc accaatgatg aattgatcgc ataatccaat agcgtcattc
     1201 cttcaaaggc catgcgcatg gatcggcttt tatcgatcag gcaatacacc tgttgtgctt
     1261 tttcatcttc gtactgattc accattaatt ccgaccgccg agcagatgct ttccagttga
     1321 tgttgcggta atcatcgccg ctgacgtaat ttttgatctg ctcaaattcg gtactttgtc
     1381 caagtcggcg aacgcgtttg attccttctg taagcgttag tttactgaat acttttagtt
     1441 cgtattgccg catctgtaaa acggaaggaa aaaccgccat ttcctcttct gccggaatgg
     1501 taatgcggcg ctgcaccagt ccaattattc ctgatgcaaa aatccggata tcaccaaaac
     1561 gatgagcccc tctttcggtt ggacgaatcg tatagcggac aatctccgaa tcgcccggac
     1621 caagctcggt gtaaatacga agatcacgta actgcaattg ctcaggtgtt tcatcggtga
     1681 tttcaatttt taccgatgta ttcattctgc tggcaacacg gattcttatt tctacttcat
     1741 ctcccagagt aagtaaagga ggaagaagtc gtttggcatg aacgcttgat ttggataaaa
     1801 agagaagaaa gacatcaagt acaagcaatg aaaatgcaag caagaccgat gccagtgcta
     1861 ttgggaaaat ttgcgggaaa ggataactca ggatgaataa actgatgcag cttatcatca
     1921 ggaggaaaaa tcttccctgc aaataactat ctctgagaat acgaatcacc tggggacttc
     1981 aattttttgt agaatatcct gtatgatatc ttctgttcgc aaaccttcca tttctgcttc
     2041 cggagagagc acgatacggt gattcaacac atgattcgcc aggaattgca catcgtccgg
     2101 aatgataaaa tctcttccgc gaattgctgc aagtgttttg gcagatttaa gcaacgcaag
     2161 cgatgcacgc ggtgatgcac ccaggtacaa ttttgcgtgt tcgcgggtgg agtgaatgat
     2221 gtcggcgatg tatttgatga gctgatcttc tacttttacg gtgcggatcg tttcctgaat
     2281 ttctttgagc actgcaactg taaaaattgc ttttatctcc gaaagatccg gtgatttgat
     2341 ctcgtctttg tatcgcaata aaatttcttt ttcttcagtc aacgttggat aatgcagttt
     2401 tacccggaaa aggaaacgat caagctgtgc ttcgggcaga cggtaggttc cttcctgctc
     2461 tatgggattt tgtgtagcta acaccaggaa aggaaactcc atactgtgtg ttactccgtc
     2521 ataggtaatc tgacgttctt ccatcacctc aaacaatgca gcttgtgttt ttgccggtgc
     2581 gcggttgatc tcgtcgatca atatgatatt gctgaataca ggtccgcgtt taaactgaaa
     2641 attcccgtct ttctgattat aaacggatgt tccgataata tcactcggca tcatatctgg
     2701 tgtaaactga atgcgtgcaa aatgtgccga taatgttttg gaaaaaactt ttgcggtgag
     2761 cgttttggca attcccggaa caccttctaa caggatatgt ccgttggaaa ggattcccac
     2821 cagcagtagt tcgatcattt catcctgacc aattacatat tttcggatct ctgtccttgc
     2881 ctgttgtatg ttttcgcgga gttgtgccaa ccgggctccg gctccattgt cgaaactgaa
     2941 gcttcctaag ggtgtgtttt gctcttccat ttatttacag tttttataaa aatactcggt
     3001 gagttggtga attgcaatga attgttcgtc actcagtcgg atttgctctg ctccgttttt
     3061 cagtgactta aataattcgt ttagcttttc ctgcgaaatt cccgatttta cagctaaaga
     3121 acgaatgaaa gcttcgtcgg ctgttcttga ttttaggtaa taccgctcgc gaataaaatt
     3181 catcaataat cgctgttttt gtattaccag atactggtgt ttgccctgtt gaaaatacaa
     3241 ttcgctcagt gttgcagcga aattaagcgt tgaattctca atctttccac gggctggaat
     3301 gggtttctgg cgacgcttaa tccggaaaag gatgaacaac aatagcgtga tgagtgtcat
     3361 gtaataagcc caggataacg attgaacact cagaataaat tgaagcggac tcgttcttga
     3421 tgttccggtt ggtgcttcat tatcgcctcc ttctgtatgc tggcgcggtt gaaatccttc
     3481 atcaacatac atgagatcta cgggtggcag gtacgagaga atcttttcga aatgttcgac
     3541 attctcttcc ctgatcaatg agatattggt gaatgcgtag ggaatgaaat ggatgaaaaa
     3601 tgcaccttca ccataattga tcctcacata aacaaagcgt tgctcatcct taagaacttg
     3661 taattctgtg gcttcaccta caaactcatt ttcgaaaatt gcattttcca aagatgcgga
     3721 atccaattct ccccgatcag tgtcgaagta gagtgaat
//
LOCUS       DAOMYG010000319         3753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8758_length_3753_cov_8.274202, whole genome shotgun sequence.
ACCESSION   DAOMYG010000319 DAOMYG010000000
VERSION     DAOMYG010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8758_length_3753_cov_8.274202"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="PK610_10180"
     CDS             <1..931
                     /locus_tag="PK610_10180"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRJ36346.1"
                     /translation="MEVPVSYQKIVVVDSKAEMFELIGNGNRSLYISSPLSKLRDYQP
                     VNAGLFTSRFEISVMDYLTMVQWQMDESPLPEIDWKSLLPDTNRMEPKTKRCISHFYH
                     QNDNGMAFTQTFSWAGASGKIYVYFSPSMKNDLNTQKALNYPVTGISHEQATIFCQLL
                     SMYFTNEINPFSNHSLALRFRLPSVGEWEEFASVGLPNETMKKNGRLDSLNDKGCQLF
                     HYNFSFSCASVESFLKERGEGMAYVSDYFPNHLGLNNVFGNAAEMMREKGIAKGGSIA
                     HYAIKASVDQYLEYQQAQPWLGFRPVAEIYLIE"
     gene            934..1443
                     /locus_tag="PK610_10185"
     CDS             934..1443
                     /locus_tag="PK610_10185"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ36347.1"
                     /translation="MEKANFQLDDKFFEADLLIKENRIADAKQMLLEIIAEAPDHGMS
                     HNHLGWIYETKYNDYVRAENHYKAALEMTPGYVAVYYNYAILLSTLGKYEEMEKLLER
                     ALTTPGINKATIYNEYGIMREAQGRYAEAISSYQNYIRHLYDNKLIETAQASIQRCKT
                     KMEILGHQP"
     gene            1440..2075
                     /gene="nth"
                     /locus_tag="PK610_10190"
     CDS             1440..2075
                     /gene="nth"
                     /locus_tag="PK610_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRJ36348.1"
                     /translation="MKKPEKVDFITTTLNKLYPNPPIPLDHKDAYTLLIAVLLSAQCT
                     DVRVNQITPLLFKKADTPKKMVKLTVDEIREIIKPCGLSPAKSKAIHRLSEILLEKHN
                     GIVPDNFEDLEHLPGVGHKTASVVMCQAFDVPAFPVDTHIHRLIWRWGLSNGKNVEQT
                     EKDCKRLFPEKSWNKLHLQIIFFGRKYCPARGHQAETCPICSKIGRKELFS"
     gene            2072..2374
                     /locus_tag="PK610_10195"
     CDS             2072..2374
                     /locus_tag="PK610_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36349.1"
                     /translation="MSIDNVLLISVPTVLLLLLFWYVVKSEKPHEEVESDEEDQMLYD
                     PLTGKKITLEEAEAGMSISEEEEEELLKQVDFEDVELESDKENSEKKTTTPPESEV"
     gene            2395..2979
                     /locus_tag="PK610_10200"
     CDS             2395..2979
                     /locus_tag="PK610_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36350.1"
                     /translation="MKTLLIAALFSFNSLPEKDCIHAELQKKNFTTCVSAEENKLYNL
                     ITEYRKSKKLPVIPLSKGLTFVARTHANDLQTNRPDKNQCNMHSWSNKGSWSSCCYTE
                     DHKQANCMWNKPKELSNYPGYGYEISANSGGTMTAKTALDVWKKSSSHNSVIINSGQW
                     KKKWNAIGIGISKNYAVVWFGNETDPDGVPGNCQ"
     gene            complement(3281..>3753)
                     /locus_tag="PK610_10205"
     CDS             complement(3281..>3753)
                     /locus_tag="PK610_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ36351.1"
                     /translation="HMVKDSQLAEDIFQDAFVKVVHTLKSGKYNEEGKFLPWVMRISH
                     NLIIDTFRKNAKMPTVEPRDEDHDIFANLRVYDQNIQDLMINDQILKDVRMLIDYLPD
                     EQKEVVMMRHYSDMSFKEIAEATNVSINTALGRMRYALINLRKMVEDKHMVLES"
BASE COUNT         1220 a          781 c          756 g          996 t
ORIGIN      
        1 gatggaagtt ccggtttcct atcaaaagat cgttgttgtg gactcaaaag cagaaatgtt
       61 tgaactgatc ggaaacggaa acagaagtct atatatcagc tcccctctca gcaagttaag
      121 agattatcaa cctgtaaacg ccggactatt tacatcccgc tttgaaattt cggtgatgga
      181 ttaccttacc atggttcagt ggcagatgga tgaatctccg cttccggaaa tcgattggaa
      241 atcactttta cctgatacca atcgaatgga accaaaaacc aaacgttgta tcagtcattt
      301 ctaccatcag aacgataatg gaatggcctt cacacaaaca ttctcatggg ccggtgcttc
      361 cggaaaaatc tacgtgtatt tttcaccctc catgaaaaat gatctcaaca cgcaaaaagc
      421 ccttaactac cctgtaaccg gaatctcgca cgaacaggcc accatttttt gtcaattgct
      481 ttcaatgtac ttcaccaacg aaataaatcc cttttccaat cattcccttg cacttcgttt
      541 tcgcttgcct tctgtaggtg aatgggaaga gtttgcttca gtaggattac ccaatgaaac
      601 gatgaaaaaa aacggaagac tggatagtct taacgacaaa ggttgtcagc tgttccacta
      661 caatttcagt tttagctgcg ccagtgtaga aagttttctc aaagaaaggg gtgaaggaat
      721 ggcctatgtt tcggattatt ttcccaatca tttgggtttg aacaatgtat ttggaaacgc
      781 agccgaaatg atgcgcgaaa aaggcattgc aaaaggagga agcattgcac actatgcaat
      841 aaaagcatct gtcgaccaat acctggaata ccaacaagct cagccatggc taggatttcg
      901 tcctgtagct gaaatttatt taattgaata attatggaga aagcaaactt tcagctcgac
      961 gataaattct tcgaagcgga tttactgatc aaggaaaaca gaattgcaga tgccaagcaa
     1021 atgctgcttg agatcattgc agaagcaccg gatcatggaa tgtcacacaa tcatttgggt
     1081 tggatctacg aaaccaaata caatgattat gtgcgtgccg aaaaccatta caaggcagca
     1141 ttggaaatga ccccaggata tgttgcggtt tattacaatt atgcgattct gttatcaacg
     1201 cttggcaaat acgaagagat ggaaaaactg cttgaaagag cacttaccac tcccggaatt
     1261 aataaagcaa ccatttacaa cgaatatgga attatgcgcg aagcacaagg caggtatgca
     1321 gaagccattt cttcgtacca aaattatatt cgccacctat acgataacaa actgatcgaa
     1381 acggcacagg cttctataca gcgctgcaaa acgaaaatgg aaattcttgg tcaccagcca
     1441 tgaaaaaacc ggagaaggtt gatttcatca ccaccacact gaacaagctg tatcccaatc
     1501 cgcctatacc gcttgatcat aaggatgcct atactttgct gattgctgtt ttgctttctg
     1561 cgcagtgtac ggatgtgagg gtaaatcaaa ttactccatt gctttttaaa aaagccgata
     1621 cgcccaaaaa aatggtgaag ctgacagtcg atgaaatccg tgagatcatc aaaccgtgtg
     1681 gattgtcgcc tgcaaaatca aaagccattc accgactttc agaaattctt ctcgaaaaac
     1741 acaatggcat tgttcccgat aattttgaag acctcgaaca tttaccgggt gttggtcata
     1801 aaacggcatc agtggtcatg tgtcaggctt ttgacgttcc cgcttttccc gttgatacac
     1861 atattcaccg gctcatttgg agatggggtt tgagcaacgg aaaaaacgtt gagcaaacag
     1921 aaaaagattg caaacgactt ttcccggaaa agagctggaa caaattgcat ttacaaatca
     1981 tcttcttcgg aagaaaatat tgtccggcac gcggacatca ggctgaaacc tgtcctatct
     2041 gttcaaagat cggacgcaaa gaattatttt catgagtata gataatgtat tactgattag
     2101 cgtacctaca gtattgctgt tgcttttatt ctggtatgtt gtaaaaagcg aaaaaccaca
     2161 tgaagaagtg gaatcggatg aagaagatca aatgctatac gaccctctta ccggaaaaaa
     2221 aataacgctc gaagaagccg aagcaggaat gagtatcagc gaagaggaag aagaagaatt
     2281 attgaaacaa gtcgattttg aagatgtaga actggaatcg gacaaagaaa attccgagaa
     2341 aaaaacgacc acccctcctg aatctgaagt ataaactctt caacttcctt acttatgaaa
     2401 acactattaa ttgccgcact ctttagcttt aacagtctgc ctgaaaagga ttgtattcat
     2461 gcagaattgc agaagaagaa ttttacaacc tgtgtatcgg cagaagaaaa taaactgtac
     2521 aacctcatta ccgaataccg gaaatcaaaa aaactaccgg taattccttt atcaaaagga
     2581 ttaacctttg ttgcgcgcac acatgccaat gatttacaaa ccaacaggcc cgacaaaaac
     2641 caatgcaata tgcacagttg gtcaaacaaa ggatcatggt cgtcctgctg ttataccgaa
     2701 gaccacaagc aagccaattg catgtggaac aaacctaaag agctaagtaa ttatccgggt
     2761 tatggttatg aaatctctgc aaattcgggt ggaacaatga cagcaaaaac tgcattggac
     2821 gtttggaaaa aaagttcatc gcacaatagt gtcatcatca attccggtca gtggaaaaag
     2881 aaatggaatg caattggaat cggaatttct aaaaactatg ctgttgtatg gttcggaaat
     2941 gaaaccgatc ctgatggtgt tcccggtaat tgtcaataaa aaaaaatgcc tccgattgga
     3001 ggcatttttt aactgttaac tgttgcgaat gttccaacgg tttcacttga atgcacttca
     3061 agcgaacgtg aaaagcgcaa aatacgatca actacatctt ttggagcctt gtatggctgt
     3121 tctatccgat tttggcaata gcacgtattg caatttttcg aatcggaaga ggaaatgaaa
     3181 gaagtaggaa tttctggagt aaagctttgc ttcataggcg ttacggcgtt taaagaataa
     3241 acgcaatcac cggaagcata ttgcccgctt cggaaaaaaa tcagctttcc agaaccatat
     3301 gtttgtcttc caccatttta cggaggttta ttaacgcgta acgcattctt ccgagagcag
     3361 tattgatact gacattggta gcttccgcaa tttccttgaa actcatatcc gaataatggc
     3421 gcatcatcac cacctccttc tgttcatcgg gcaagtaatc aataagcata cgtacgtctt
     3481 ttaggatctg gtcattgatc atgagatcct gaatattttg gtcgtacacc cgaagattgg
     3541 caaaaatatc atgatcctca tcacgaggtt caaccgtagg catttttgca ttttttcgga
     3601 aggtatcgat gatcaggtta tgggaaatcc gcatcaccca aggcagaaat ttaccctcct
     3661 cgttgtactt tcctgatttt aaggtatgta ctaccttaac aaaagcatcc tggaagatat
     3721 cctctgcaag ctgactgtcc ttaaccatat gcc
//
LOCUS       DAOMYG010000320         3722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8869_length_3722_cov_9.382602, whole genome shotgun sequence.
ACCESSION   DAOMYG010000320 DAOMYG010000000
VERSION     DAOMYG010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8869_length_3722_cov_9.382602"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /gene="lpcA"
                     /locus_tag="PK610_10210"
     CDS             complement(<1..546)
                     /gene="lpcA"
                     /locus_tag="PK610_10210"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HRJ36352.1"
                     /translation="MSISRVQQHFIEAKQALESFMENENNFRAIEVAGGLMADALKMG
                     KKVISCGNGGSMCDAMHFAEELSGRYRDDRKALAAVSISDPSHISCVGNDMGFDQIFS
                     RYIEALGQPGDVLLAISTSGNSGNVLKAIAAAKAKGIIVVGLTGKDGGKMSGLCDVEI
                     RAPHSAYADRAQEIHIKVIHAL"
     gene            647..1087
                     /locus_tag="PK610_10215"
     CDS             647..1087
                     /locus_tag="PK610_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36353.1"
                     /translation="MKHSVLIALLAVFTLPAVAQTAQPKKELTCYEAYKKEFDERGAY
                     TVDDGKYTGVIIVVATADGTDCVSGKVQVEGGNVTNIWLQYEDNTFEFLERKYVGNKN
                     PKIVNGITEPLKTTNGETIYVIFVDKIKPKKKQLKKVTGPGNGF"
     gene            1111..1917
                     /locus_tag="PK610_10220"
     CDS             1111..1917
                     /locus_tag="PK610_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36354.1"
                     /translation="MRLRLALLIVLPALAALVYSFTATIALPEDDKYKVIKVNGEIIV
                     KRSGKALVTGDELLASTPLDFKTTESRAAVVSPTKGRFVLTAASQGGKTNLVPGMNNV
                     ASRSGALLNMIDLQNHFTGDYVILDRNMLKISKEAYPMDEKNFFFLRYVYNGETINKR
                     LTFRGDSLSMERAAIFTIDNKPVEVKDQLSCSLFYRVSESNENRLISSFTAVFPDLNA
                     LKSEFQIVFDNNAGKKTSEKVDEVMGYLTEFYGKADKDNLNRWLNSNFKF"
     gene            complement(1981..3024)
                     /locus_tag="PK610_10225"
     CDS             complement(1981..3024)
                     /locus_tag="PK610_10225"
                     /inference="COORDINATES: protein motif:HMM:NF015272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HRJ36355.1"
                     /translation="MSRPPVHYSDYLQLEKILDAQHPESDKEKVEAHDEMLFIIIHQA
                     YELWFKQILHEIDSMAAIMGKPVVNDNSPELQTVVHRMERVVTILKVLVHQIDILETM
                     TPLDFLDFRDLLRPASGFQSWQFKITEAKLGLKFQHRYGQEYYVSQLRPEQVKLIKDI
                     EQQPTLLELVNKWLERMPFFEDDTLWKNYKKHFPEEDGQHIFWSDFKNIYASTLVDGE
                     KSNLAHFYEMFSDNAEGTRELSPKACRSALFIMLYRGYPMLQTPYRLLNNLLEVDEQL
                     STWRFRHVNMVHRMIGSRTGTGGSTGKEYLKNAMDKHYIFKEVSALTSFLIERRKLPH
                     LSQEMDKRLGFHH"
     gene            complement(3110..>3722)
                     /locus_tag="PK610_10230"
     CDS             complement(3110..>3722)
                     /locus_tag="PK610_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36356.1"
                     /translation="KTALETVFSRALIKVESDLTGAARGLCGSQPGLAVILGTGSNSC
                     QYDGKSITHQRPSLGYILGDEGSGAWIGKTLLNAHFSDELPKAISDSLTQRLKVEIDD
                     VIEHVYRKPFPNRYLATYSKFVFQNSKNPAMVQLVRTGFDAFFRKQLLHYPNKELPLH
                     ATGSVAFFFSAIFKDVAAEHGFTTGMITESPVAGLALYHLGEV"
BASE COUNT         1031 a          785 c          839 g         1067 t
ORIGIN      
        1 caaggcatgg atcaccttaa tgtggatctc ctgtgcacgg tcggcatagg ctgaatgggg
       61 tgctcggatt tctacatcgc atagtccgct cattttacct ccgtcttttc cggtcagacc
      121 aacaacaata attccttttg ctttcgccgc tgcaatggct ttcaatacat taccggaatt
      181 accgctggta gaaatggcaa gaaggacatc gcccggctga ccaagcgctt cgatataccg
      241 cgaaaaaatc tgatcgaatc ccatatcgtt acccacacag ctgatgtggg agggatcgga
      301 gatagagaca gcagccagtg cttttctatc gtcgcggtag cgtcctgata gctcttccgc
      361 aaaatgcata gcgtcgcaca tggagccgcc gttcccgcag gaaatcacct ttttacccat
      421 ttttagtgca tcagccatca ggccgcctgc tacttctatg gcacggaaat tgttttcatt
      481 ttccatgaag gattcaagcg cctgtttcgc ttcgatgaaa tgttgctgta cacgactgat
      541 gctcatgcct gtttgatttg ttgacaggtc aaaagtagtg gaacctgtac tattttcgaa
      601 acgaaaaaaa tgcttatgtt tactgttcga taaaaccata ccagacatga aacacagcgt
      661 actcatcgca cttttggctg tttttaccct gcccgcagtg gcacaaactg cgcagcctaa
      721 aaaggaactg acctgctacg aagcgtataa gaaggaattc gacgagcgtg gggcctatac
      781 cgttgacgac ggtaaataca ccggagtaat catcgtggta gctactgccg acggaacgga
      841 ttgtgtgtcc ggaaaagttc aggttgaggg tggaaatgtc acgaacatct ggcttcagta
      901 cgaagacaat accttcgaat tcctcgaaag aaaatatgtg ggcaacaaaa atcccaagat
      961 tgttaatggt atcactgagc cactgaaaac aaccaatggc gaaacgattt atgtgatctt
     1021 cgtggataag atcaagccca agaaaaaaca attgaaaaag gttaccggtc cgggtaacgg
     1081 attctgattt ttcaattaat tctaaaggct atgcgtttac gacttgccct ccttattgta
     1141 ttgcctgcct tggcggctct tgtctattct tttaccgcaa caattgcttt gcccgaagat
     1201 gataagtata aagtgatcaa ggtgaatggt gagatcatcg taaagcgctc cggtaaagca
     1261 ctggtcaccg gcgatgaact gctggcctct actcctctgg attttaaaac gactgaatcg
     1321 cgggctgctg ttgttagtcc cacaaagggt cgttttgtac ttactgctgc atcacaaggt
     1381 ggtaaaacga atctggttcc tggtatgaat aacgtggctt ccagaagtgg tgccttgctg
     1441 aatatgattg atctgcagaa tcatttcacc ggagattatg ttattctcga tcgtaacatg
     1501 cttaaaattt cgaaagaggc ttatccgatg gatgaaaaga actttttctt tttgaggtat
     1561 gtctacaacg gagaaacgat caacaaacga ttgactttcc gcggagattc cctaagcatg
     1621 gaacgtgctg caatttttac catcgataat aaaccggttg aagtaaagga tcaattgtcg
     1681 tgtagtttgt tttacagggt ttctgaatca aacgaaaacc gattgatcag ttcttttaca
     1741 gcggtctttc ctgatcttaa tgctttaaaa agcgaattcc agattgtgtt tgataataat
     1801 gccggaaaga aaacgtctga aaaagttgat gaagtgatgg ggtacctcac tgagttttac
     1861 ggtaaggcag acaaagacaa cctgaatcgt tggctcaatt ccaatttcaa attctgaatc
     1921 tgaatagaaa caaaaaagcc tgatcacgcg atcaggcttt tttgtttgtt gtatattcct
     1981 ttagtgatgg aagcctaaac gcttgtccat ttcctgactc aaatgcggga gtttccttcg
     2041 ctcaatgagg aaactggtta atgcagaaac ttccttgaaa atatagtgtt tgtccatcgc
     2101 gtttttgagg tattcttttc ctgttgatcc gccggttcct gttcttgatc cgatcatgcg
     2161 gtgtaccatg ttaacgtgac ggaatctcca ggtactgagt tgttcatcta cctcaagaag
     2221 attgttcagc aaacgataag gtgtctgcaa catggggtaa ccacggtata acatgatgaa
     2281 aagtgcagaa cgacatgctt tgggtgataa ctcgcgtgtt ccttctgcat tgtcggaaaa
     2341 catttcgtaa aaatgagcaa gattgctttt ttctccgtct accagtgttg aggcataaat
     2401 gtttttgaag tcgctccaga aaatatgctg accatcttct tccggaaaat gttttttgta
     2461 gtttttccac aaggtatcat cttcaaagaa gggcattcgc tcaagccatt tgtttacaag
     2521 ctcaagtagc gttggttgtt gttcgatgtc tttaataagt ttcacctgct caggacgcaa
     2581 ttgtgaaacg tagtattcct gtccgtaacg gtgctgaaat tttaatccga gtttcgcctc
     2641 tgtaatttta aattgccagc tctgaaagcc ggatgcagga cgtaaaagat cacggaaatc
     2701 gaggaagtcc aagggtgtca ttgtttcgag gatatcgatc tgatgaacca ataccttgag
     2761 aatggtaaca acacgctcca tgcggtgcac cacggtttgc aattcgggtg agttgtcgtt
     2821 caccacgggt ttgcccataa tggcagccat tgagtcgatc tcatgtagaa tttgtttaaa
     2881 ccaaagctca taggcctggt gaatgatgat gaataacatt tcatcatgcg cttctacttt
     2941 ttctttatcg ctttcaggat gttgtgcatc gagaattttt tcgagctgca ggtaatcgct
     3001 gtaatgtacg gggggacgac tcataagaat tcagtttggt agtgcgaatg taatctgtta
     3061 gaagagaaaa actatgattt tggtctgttt taaggggaga aacagggatt tatacttcgc
     3121 ccaggtgata tagagcaagt ccggcaacag gagactccgt aatcattccc gttgtaaatc
     3181 cgtgttcggc agctacatcc ttgaaaatag cagaaaagaa aaatgcaacc gatcccgtag
     3241 catgcagtgg taattcctta ttcggataat gaagcagttg cttacggaaa aaagcatcaa
     3301 atccggttcg caccaattgt accattgcag gattcttgct gttctgaaaa acaaatttgg
     3361 aatacgttgc caaatagcga ttgggaaatg gctttcggta aacatgttca atcacatcgt
     3421 caatttcaac tttcagacgt tgtgtaagcg aatcactgat ggctttcggt agctcatcac
     3481 taaaatgcgc attcagcagc gtctttccaa tccatgcacc actgccttca tctccgagga
     3541 tataacccaa tgagggacgt tgatgagtaa tcgattttcc atcgtattga caggaattgg
     3601 atcctgttcc caaaatgaca gcaagtccgg gctgactacc gcacaaaccc ctggctgcac
     3661 cagtgagatc actttcaact ttaattaatg cacgagagaa aacggtttca agcgcggttt
     3721 tc
//
LOCUS       DAOMYG010000321         3690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8971_length_3690_cov_9.008803, whole genome shotgun sequence.
ACCESSION   DAOMYG010000321 DAOMYG010000000
VERSION     DAOMYG010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8971_length_3690_cov_9.008803"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1937)
                     /locus_tag="PK610_10235"
     CDS             complement(<1..1937)
                     /locus_tag="PK610_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HRJ36357.1"
                     /translation="MGKYKHCVLLVFLSEVMVNELRSILQRYWGHAEFRDLQEDIIRS
                     VLEGHDTLALLPTGGGKSVCFQVPALARKGMCLVVSPLIALMRDQVRNLNARGIKAIA
                     ITSAMEYREIDIALDNCIYGEVKFLYVSPERLQTALFQERLKRMSINLIAVDEAHCIS
                     QWGYDFRPPYLRIAEIRQFLPDVPVMALTATATPEVVEDIQEKLAFHRGKVFRSSFAR
                     KNLGYIVRNEENKGDRMLRICNQIQGTGIIYVRNRKRTQETALFLQKNGISADFYHAG
                     LEPAQREQKQNDWISGKTRVICSTNAFGMGIDKPDVRFVIHLDLPDSPEAYFQEAGRG
                     GRDGQMSWAIMLYDEADVFALEESVRQSFPDMDTVRRTYIALHNYYQIAIGAGLEKTA
                     EFDLNEFCHRFNLIPTVAHNAMRLLEIGEFIELSEAYSNPARIRMLMDQAHIYDFQIR
                     NKNFDAFIKLLLRSYSGLFDDFVRINEKEIAKRTGMEYEQVVRMLKKLDELKVIVYVP
                     RSDKPRITFLQNRMSERDFSLPPVVYEQRKRVAMDRMHTMTDYVRNKKSCRSTLLLKY
                     FGEDPETRCGLCDHCQRENKVGLSNIEMESITVEIKEVVSDGAIDIRKLREKLGHHSE
                     EKIKKVIRWLLDNDQLKMDEE"
     gene            1996..3072
                     /locus_tag="PK610_10240"
     CDS             1996..3072
                     /locus_tag="PK610_10240"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRJ36358.1"
                     /translation="MIAEKVSTPIRVELTKKSRLPQVDFDNIVFGRQFSDHMFMVDYT
                     DGKWHDARIVPYAPIPLSPAMHALHYGQSIFEGMKAYRNNREEIYLFRPEENFKRLNN
                     SAERMCMPQLPKEIFMDALNELIRLDQDWVPRKEGSSLYIRPFMFATDEFIGVKPSET
                     YRFMIFTCPVNSYYTEPVRVKIETVYSRACEGGVGRAKAAGNYAAALYPARLGQQEGY
                     HQLVWTDAKEHKYIEESGTMNIMFNIGDKLVTPPTSGTILPGITRNSVITIARDWGLN
                     VEETSVHVDEVINALKKGTLKEAFGMGTAATIAHIKLIGYRNKKYELSDPDSSYFSHK
                     VSKYLEDLRRGYVEDKFNWTYQVV"
BASE COUNT          952 a          886 c          815 g         1037 t
ORIGIN      
        1 ttctcctcat ccattttcag ctgatcatta tccagcaacc agcgaatgac ttttttgatc
       61 ttctcctccg aatgatgtcc cagtttttcg cgcaatttgc ggatatcgat ggcgccatcg
      121 cttactactt cttttatttc tactgtaatg ctttccattt cgatgttgga gagtcctact
      181 ttgttttcgc gctgacaatg atcgcagagt ccgcagcgtg tttcagggtc ttcaccaaaa
      241 tacttcagta ataaggtact cctgcacgat tttttattgc gcacataatc cgtcatggtg
      301 tgcatgcgat ccatcgctac ccgtttgcgt tgttcgtaca ctaccggtgg aagcgaaaaa
      361 tcgcgttcag acatacggtt ctgcaaaaag gtgatgcgtg gtttatccga tcggggaacg
      421 tacacaatca ccttcaactc atccagtttt ttgagcatgc gcaccacctg ctcgtattcc
      481 attccggtgc gtttggcgat ttccttttcg ttgatgcgca caaaatcatc gaacaatccc
      541 gaatacgatc gcagcagcag tttaatgaag gcatcgaaat tcttattgcg gatctggaaa
      601 tcgtaaatat gcgcctgatc catcaacatc ctaatgcgtg ccggattgct gtaggcttcg
      661 gataactcga tgaattcacc gatctcgagg agtcgcatgg cattgtgtgc aacagtggga
      721 ataagattga agcggtgaca aaattcattg agatcgaact ccgccgtttt ttccaatccc
      781 gccccaatgg caatctggta atagttatgc aatgcgatat aggtccgccg aaccgtatcc
      841 atatccggaa acgattgacg aacgctttcc tcgagtgcaa atacatcggc ttcgtcgtag
      901 agcataatcg cccacgacat ctgtccatcg cgtcctcccc ttcccgcttc ctgaaaatag
      961 gcttccggtg aatcgggtaa atcgaggtgg atcacaaaac gcacatcggg tttatcgatg
     1021 cccattccaa aggcattcgt agagcagatc acccgtgttt ttccgctgat ccagtcgttt
     1081 tgtttttgtt cgcgctgtgc gggctccagt cctgcatggt agaaatcggc agatattccg
     1141 tttttctgaa ggaagagcgc tgtttcctgc gttcgtttgc ggttcctcac gtatatgatt
     1201 ccggtgccct gaatctgatt gcagatccga agcatgcgat cgcctttgtt ctcttcgttt
     1261 cgaacaatgt agccgaggtt ttttcgcgca aaactgctgc ggaatacttt tcctctgtga
     1321 aatgcaagtt tctcctggat gtcttctacc acttcgggtg ttgcagtagc tgtgagcgcc
     1381 attacaggca catccggtaa aaactggcgg atttctgcaa tgcggaggta gggcggacga
     1441 aaatcgtatc cccattggga aatacaatgt gcttcatcaa cggcaatgag attgatgctc
     1501 attcgcttca ggcgttcctg aaacagggct gtttgcaggc gttcgggtga gacgtacaaa
     1561 aacttgacct cgccataaat gcaattgtcg agcgcaatat caatctcgcg gtattccata
     1621 gccgaagtga tggcaatggc cttgattcca cgggcattga ggttgcggac ctgatcgcgc
     1681 atgagggcga tgagcggaga aaccacaaga cacatgcctt tacgcgcgag tgcaggaacc
     1741 tgaaaacaga cggatttccc tccgccagtt ggtaacaggg caagcgtgtc atgtccctct
     1801 aatacagagc gaatgatgtc ctcctgaagg tcgcggaact cggcgtgacc ccaatagcgt
     1861 tgcaatatgg aacgtaactc gttgaccatt acctcactca aaaataccaa taatacgcaa
     1921 tgcttgtatt ttcccattca cctaaagtaa aatgcttata ttcgtaacaa caccagcacc
     1981 aaaaacacat cggttatgat tgcagaaaaa gttagcactc cgatccgtgt ggagctcacc
     2041 aaaaagagcc gcctcccaca ggtcgatttt gataatattg ttttcggacg tcagttttcc
     2101 gaccatatgt tcatggtcga ttacacggat ggaaaatggc acgatgcccg cattgttccc
     2161 tacgctccta ttccgctgag tcctgctatg catgctttgc attatggtca gtccatcttc
     2221 gagggaatga aagcataccg caacaaccgc gaagagattt acctattccg tccggaggag
     2281 aatttcaagc gactgaacaa ttcagccgag cgcatgtgta tgcctcagct tcctaaagag
     2341 attttcatgg acgccctcaa tgaactgatt cgtcttgatc aggattgggt tccccgcaag
     2401 gaaggatctt ccttgtacat ccgtcccttt atgttcgcca cggatgaatt cattggagtg
     2461 aaaccatcgg agacttaccg cttcatgatt ttcacctgtc ccgtaaactc gtattacacc
     2521 gagccggttc gtgtgaaaat tgaaacagtg tattcccgcg cctgcgaagg aggagttggt
     2581 cgcgccaaag cagcaggtaa ctacgcagct gccctctacc ctgcacgatt gggtcagcag
     2641 gaaggctacc atcagcttgt ttggaccgat gccaaggaac acaagtacat tgaggaatcc
     2701 ggcaccatga acatcatgtt caatatcggc gataagctcg ttactcctcc caccagtgga
     2761 accattttgc cgggtataac acgtaatagt gtaattacaa ttgcgcgcga ttggggattg
     2821 aatgtagaag aaacgagtgt gcacgtggat gaagtgatta atgcgctcaa gaaaggaacc
     2881 ctcaaggaag ccttcggaat gggaacagct gctaccattg cacacatcaa actcattggt
     2941 taccgcaaca aaaaatacga actctccgat cccgattctt cttacttctc tcacaaggtg
     3001 agcaagtacc tcgaagatct gcgcagaggt tatgtcgaag ataaattcaa ctggacctac
     3061 caggttgtat aaatgaaaag tcccgcatgt gcgggacttt tttttataat aatttttgtt
     3121 ccggtatttg ccaattctta aactcctctt atttttacat tcatctatga tcaagagcct
     3181 gtttgttttt ttaataacct gtgttatgat ggctcaactc ttttcaggca gcctgatctt
     3241 gtgtagtttt tattttaatc aaaaagccat tgcctcggaa aagtgcatca ataagaacaa
     3301 gcccagactt aaatgcaatg gtaagtgcta tttagcaaag caattgctgg aagaaaccag
     3361 taagggaaaa aaatatccgg agttcccctc ttttcggaaa tatccggaat tgaatcaaaa
     3421 gttgaattcg ggatttccgg aattgcatgg ttatattatt ggtactaccg gatttttcta
     3481 tcaatggcaa atagagaatc gattttctcc accgtttcca cctccccctg aactgagctg
     3541 atccattttc tctgttggtt ttaacctaaa cagatcaaaa cttagtgtag aatttattga
     3601 taaactcaga tgaactcatt ttttctgtct gtgtcagtca ttgtactggc cgttgtttct
     3661 cgtattgcac atcttgaaac ttcaacccta
//
LOCUS       DAOMYG010000322         3689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_8974_length_3689_cov_7.066318, whole genome shotgun sequence.
ACCESSION   DAOMYG010000322 DAOMYG010000000
VERSION     DAOMYG010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8974_length_3689_cov_7.066318"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PK610_10245"
     CDS             complement(<1..228)
                     /locus_tag="PK610_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRJ36359.1"
                     /translation="MKIALIGYGKMGKEIESIALGKGHEISFKVTSANAGFSAADLKD
                     SDVAIEFSKPDVVISNIEKCFDANIPIVVGTT"
     gene            complement(240..773)
                     /locus_tag="PK610_10250"
     CDS             complement(240..773)
                     /locus_tag="PK610_10250"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HRJ36360.1"
                     /translation="MSPAGLVLILMLSGSPANDSSRTERPAFDTSHSPKKAMLLSLCL
                     PGAGQIYNRKFWKLPLVYGALGTSAWFFFDNNSQYKKHRTEYLFRLNNNGAANDADLT
                     LYSEGQLRTLTDQYRRWRDLSVVAFAAFYALQVVDATVDAYLWRHDTSRDLSYFIRPS
                     FIGATGGMAGLRIGFKF"
     gene            complement(773..1693)
                     /locus_tag="PK610_10255"
     CDS             complement(773..1693)
                     /locus_tag="PK610_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRJ36361.1"
                     /translation="MSKQKKAALGRGLSALLQSSETDITSKYESKKFSEAVGSVALVS
                     VEKIEANPFQPRQHFEKQALVELTQSIREHGIIQPLTVRKLGNDRYQLISGERRFKAS
                     QIAGLTEVPVYIRIANDQEMLEMALIENIQRQNLDAIEVAIGYQRLIEECKLTQEQLS
                     ERVGKERSTVTNYLRLLKLPPEIQLAIRERKLTMGHARAIVSLTSERDQVKLFNEVVD
                     NDLSVRKTESRAKDILQGRDEAPSEDTSKKSGKSANGLSLPQQKIREDLMTRLNATVD
                     IKSTPKGSGKIIIEFRSEGDLERIIELLDL"
     gene            complement(1690..2496)
                     /locus_tag="PK610_10260"
     CDS             complement(1690..2496)
                     /locus_tag="PK610_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ36362.1"
                     /translation="MGKIIAVVNQKGGVGKTTSAINLSACFGALEFKTLLVDADPQAN
                     ATSGVGFDPKNVKTSIYECLINDTDPRDIILEANNPNLDLLPAHIDLVGAEIELINLP
                     NRELMMKRALHKIKNEYDFIIVDCSPSLGLITVNALTAADSVIIPVQCEYFALEGLGK
                     LLNTVKIVQSRLNPELSIEGILLTMYDTRLRLSNQVVDEVKTHFQQLVFDTIIHRNTT
                     LGEAPSHGETIIMHDATSKGAINYLNLAREILQKNEMTRISEADKTIEVE"
     gene            2517..2738
                     /locus_tag="PK610_10265"
     CDS             2517..2738
                     /locus_tag="PK610_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36363.1"
                     /translation="MKTTLQEIFGFFVQRNNFPQKIKIAKGRVLKALKTGEILNKNLL
                     IGVLSTICIENDHHNRIHPSQKQLHSQNG"
     gene            2743..3201
                     /locus_tag="PK610_10270"
     CDS             2743..3201
                     /locus_tag="PK610_10270"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRJ36364.1"
                     /translation="MKTRLQQRGQAASVLDLRLLPRDFMWKKIEGEEHPEFDYLIGEH
                     IRDVHKFIFVIPEYNGSFPGMVKTFIDAVPPRFFYTKKAALIGIASGRAGALRALDDM
                     TGVLHYLQVEVLSDKPKFPGIDKILNDSGSINDPDASVRLDKCIDKFLAF"
     gene            3289..3609
                     /locus_tag="PK610_10275"
     CDS             3289..3609
                     /locus_tag="PK610_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36365.1"
                     /translation="MAEISQKQRTRNWTKLIRNKYVLTSLVFLVWMTFVDNNDFFRQY
                     KVYSKKNELNRELNRRKGMIRETNESLQQLRDRKHLEKFAREQYLFKREGEEIFVVVA
                     KDQN"
BASE COUNT         1049 a          810 c          762 g         1068 t
ORIGIN      
        1 tgtggttcca acaacaatgg gaatatttgc atcgaaacat ttttcaatat tggagattac
       61 cacatccggt ttactgaatt cgatagcaac atccgaatct ttaaggtcag cagcagaaaa
      121 tccggcattg gcagatgtca ctttaaatga aatttcatgt cccttaccca gtgcaatgga
      181 ctcaatctcc ttgcccattt ttccgtatcc aatgagtgca atcttcataa tgcgtaaaat
      241 tagaatttaa acccaatacg caatcccgcc attccaccgg tggcgccgat aaaggacgga
      301 cggatgaaat aggaaagatc gcgtgaagta tcatgccgcc acaaataggc atctactgtt
      361 gcatctacca cctgcagtgc ataaaatgcc gcaaaggcaa ctacagacag atccctccat
      421 cggcgatact gatcggtaag cgttcgtaat tgtccttccg aatataatgt cagatctgca
      481 tcgtttgcag ctccattgtt attcagacga aaaaggtatt ctgtcctgtg ttttttgtat
      541 tgcgaattgt tatcaaaaaa aaaccaggca gaagttccca acgcaccata taccaagggt
      601 aatttccaaa atttccgatt gtatatttgt cccgcaccgg gcaggcacaa ggacaataac
      661 attgcttttt tgggagaatg actagtgtcg aaagcaggac gttcggtccg gctgctgtcg
      721 ttggcaggtg aaccggaaag cattaggatc aataccaatc ctgcaggcga catcagagat
      781 caagcaattc aatgatccgt tccagatcac cttcactgcg gaattcgata atgatttttc
      841 cgctgccttt aggtgtgctc ttaatatcca cggttgcatt caaacgtgtc ataaggtctt
      901 cacggatttt ctgttgtggg agcgataatc cgtttgcaga tttaccgctc ttcttggatg
      961 tatcttccga gggagcttca tccctgccct gcagaatatc ttttgcacgg gattcagttt
     1021 tacgaacgct caggtcgttg tctacaactt cattgaataa tttaacctgg tctctttcgc
     1081 tggtcaggga aacaatggcg cgcgcatgac ccatggtgag tttgcgctca cgaattgcaa
     1141 gctgaatttc aggtggtaat ttaagcagac gaaggtaatt cgttacagtc gaacgttctt
     1201 tacctacacg ttcgctgagt tgttcctgcg ttaatttgca ttcctcaata agacgctgat
     1261 aaccgattgc cacctctatc gcatcaaggt tctgacgctg gatgttttcg ataagggcca
     1321 tttccagcat ttcctggtcg ttggcaatac ggatgtaaac cggaacttca gttaagccgg
     1381 caatttgtga agctttaaat cgacgttcac cagaaattaa ctgatagcga tcgttaccca
     1441 gtttgcgcac agtgagcggc tgaatgatgc cgtgttcgcg aatggattgc gttaattcaa
     1501 ccaaagcctg cttttcaaaa tgctgacgag gctggaaagg attcgcttcg atcttttcaa
     1561 ctgatacgag ggcgacagat cctactgctt cagaaaattt tttgctttcg tatttagacg
     1621 tgatgtcggt ttccgaactt tgtaatagtg cacttaagcc tcttccgaga gctgctttct
     1681 tttgtttgct cattctactt caattgtttt atctgcttca ctaattcttg tcatctcatt
     1741 tttttgcagg atctcacgtg caagattgag ataatttatc gcacccttgc tggtagcatc
     1801 gtgcataatg atggtttcac catgactcgg agcttctcct aaagttgtgt tacggtgaat
     1861 gatcgtatca aacaccaatt gctggaaatg cgtttttact tcatcaacaa cctggttact
     1921 taggcgcaga cgggtatcgt acatcgtgag caaaatacct tcaatgctga gttcaggatt
     1981 caggcgcgat tgcacgattt taaccgtatt gagcaattta cccaaacctt ccagtgcaaa
     2041 atattcgcat tgtacaggaa tgataaccga atcggcagca gtgagtgcat taaccgtaat
     2101 caatcccaat gaaggcgaac aatcgacgat gataaaatca tattcgtttt tgattttatg
     2161 caatgcccgc ttcatcatca attcccggtt gggaagatta atcaactcaa tttcggcacc
     2221 aacaagatca atgtgagccg gaagcaaatc gagatttggg ttattggctt ccaaaatgat
     2281 atcgcgtgga tcggtatcat tgatcagaca ttcataaatg cttgtcttca catttttcgg
     2341 atcgaaaccc acacctgaag ttgcatttgc ctggggatct gcatccacta gcaatgtttt
     2401 aaactccaat gcaccaaaac atgcactaag attaatggct gaagtggttt tacctactcc
     2461 acctttctga ttcacgaccg ctatgatctt tcccattcct tctatttcaa tgatttatga
     2521 aaacaactct tcaggagatt tttggtttct ttgtgcaaag aaacaatttc ccgcaaaaga
     2581 taaagatagc taaaggaaga gtcctgaaag ccttgaaaac aggggagatt ttaaacaaaa
     2641 atctgttaat tggggtttta tcaacaattt gcatcgaaaa tgatcaccat aatcgtatcc
     2701 acccatcgca aaaacagcta cacagccaaa atggctgagc tcgtgaaaac caggctccag
     2761 caacgcggac aagcggcgtc tgttcttgat cttcgattac ttcccaggga ttttatgtgg
     2821 aaaaagatcg aaggcgaaga acaccctgag ttcgattatc tcattggaga acatattcgt
     2881 gatgttcata agttcatttt tgtaattccc gagtacaacg gaagttttcc gggtatggtt
     2941 aaaaccttta tcgacgctgt tcctccccgc tttttctaca caaaaaaagc tgcattaatc
     3001 ggcattgcct ccggaagagc gggtgcattg agggctctgg atgatatgac aggagttttg
     3061 cactacctgc aagtggaagt gttgagcgat aaaccaaaat tcccgggtat cgacaagatt
     3121 ctgaacgatt caggatctat caacgacccc gatgcttctg ttcgacttga taaatgcatt
     3181 gataaatttc ttgcctttta gatgtttcgg taattgttaa caccgggtta tggaaagaac
     3241 agaatcaact gcctccacaa gctactgctg cacacttaca tttgtttaat ggcagaaatc
     3301 tcacaaaaac aacgcacccg taattggaca aaactgatca ggaacaaata tgttctgacg
     3361 agtttggtgt ttttggtttg gatgactttt gtcgacaaca acgatttctt tcgtcaatac
     3421 aaggtttaca gcaaaaaaaa cgagctgaac cgcgaattga accgacgcaa gggaatgatc
     3481 agagaaacaa atgaaagtct gcaacaactt cgcgaccgca aacacctcga aaaatttgca
     3541 agagaacaat acctctttaa acgggaaggt gaagaaattt tcgttgttgt agccaaagat
     3601 cagaattgat cgtacaccaa ccttttccgg tcgtttttcg tatatttttt tgagcagttt
     3661 aaacattcat cccatggcca gatatacac
//
LOCUS       DAOMYG010000323         3672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9036_length_3672_cov_12.007188, whole genome shotgun sequence.
ACCESSION   DAOMYG010000323 DAOMYG010000000
VERSION     DAOMYG010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9036_length_3672_cov_12.007188"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..286)
                     /locus_tag="PK610_10280"
     tRNA            complement(204..286)
                     /locus_tag="PK610_10280"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(251..253),aa:Leu,seq:caa)
     gene            563..1516
                     /locus_tag="PK610_10285"
     CDS             563..1516
                     /locus_tag="PK610_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRJ36366.1"
                     /translation="MEEVPVGYIIAGVFGLLTLFSLFTTVQQGQIGVVTIFGKYRRVM
                     GPGLNLKIPFIEKVMRRVSIQNRSVELGFQAVTVDQANVNFTAMLLYAVFDDSEESIK
                     KVAFKFLDERNFMQALVRTVEGSIRAFVASKKQAEILLLRREIVEEVKIQLDHTLEEW
                     GYHLLDLQMNDITFDSEVMNSMAKVVASANLRAAAENEGQALLITKTKAAEAEGNAIK
                     IAAEAEREAAILRGQAVARFREEVAKGMSGAAKEMQQANLDSSFILFSMWTEAIKNFA
                     QEGKGNLIILDGSVDGMKKTLAELSAASRLGLFDQPDGKHK"
     gene            1593..2528
                     /locus_tag="PK610_10290"
     CDS             1593..2528
                     /locus_tag="PK610_10290"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRJ36367.1"
                     /translation="MSTGIKFFAGRATQDLGRKVAESYGAKLGEVAVSTFADGEFQPS
                     FEETVRGKDVFIVQSTIPPTDNLFELLLLVDAARRASARKVVAVIPYFGFARQDRKDK
                     PRVSIGAKLVANLLTAAGVDRIMTMDLHADQIQGFFEVPVDHLFASTVFIPYVQNLNL
                     PNLIMAAPDTGGTKRANAYAKYLNCDLAICYKQRKKANEIDSMTVIGDVQGKDVVLVD
                     DICDTAGTLTKAADMMLERGATSVRAICTHAVLSGNAYERIEKSGITELIVTDTIPLR
                     QQSPKIRVITVANLFGDVINRLQNYESISSHFIIQ"
     gene            2549..3133
                     /locus_tag="PK610_10295"
     CDS             2549..3133
                     /locus_tag="PK610_10295"
                     /inference="COORDINATES: protein motif:HMM:NF004132.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HRJ36368.1"
                     /translation="MKKVSLSGSPRANVGKKDASDLRNKGLIPCVLYGGNGQTHFSIN
                     EIALKKVIWSPDVYNVELDIAGKKTNAIVKDVQFHKVSDRLQHIDFLELVPNKPVKLK
                     LPVRLTGSPEGVKKGGKLVQNYRKMNVMGAPEKLPEAIIVDVEKLDIGQNIRVKNISI
                     DGLRLLETAESVVAAVETTRNVATEAKDAAAAKK"
     gene            3152..>3672
                     /gene="pth"
                     /locus_tag="PK610_10300"
     CDS             3152..>3672
                     /gene="pth"
                     /locus_tag="PK610_10300"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRJ36369.1"
                     /translation="MKYLIVGLGNIGSEYEDTRHNIGFSVLDAFAKASSAVFTSDRLA
                     HVAEVKFKGRTLVLIKPTTFMNLSGKAVNYWLQQEKISPENMLVITDDLALPFGKIRI
                     RPAGSDGGHNGLKDIQAVLGHNNYPRLRFGIGAEFSKGKQSDYVLGKWGEEENKTLPQ
                     RIELCCEIIKSFST"
BASE COUNT         1008 a          827 c          890 g          947 t
ORIGIN      
        1 aacgtgggtt tccagcgcat ataacgaaaa aagaatcctt cagaagatgc ttttccctaa
       61 aggtctttcg taccatcgag aaaatgatga atatctaacc acaagaccga atcgtgtatt
      121 ccaattaatc gcccaattaa caggggattc cgaaaaaaat aaaagccgga taagcagttt
      181 ttttcttgct atatccggct cagtacccgg ggtcggactc gaaccgacat gtccgtgaag
      241 acaatggtgt ttgagaccat cgtgtctacc attccaccac ccgggcggct tgtctgacct
      301 aatgttttag ggcagggtga tttattttac cattccactc gaagatcctg accgatcgtt
      361 agagagcgtc aggacacccg ggcgggtgat tcttatttac cattccactc gaagatcctg
      421 accgaccaat gggcgcgtct ggacaccctt gcaagggatt gggatcgcga aaatagtatt
      481 tgctgcggta atgacaaata atttcttttg gggttgtttg ccaattcgtt ggttttgcgg
      541 atattgctaa aaaaacaaaa ccatggaaga agtacctgtt ggatatatca ttgccggagt
      601 attcggttta ttgaccctgt tttcgctctt tactactgtt cagcaagggc agattggtgt
      661 agtaaccatt tttggaaagt accgaagggt gatgggaccc ggacttaact taaagattcc
      721 attcatcgaa aaggtgatgc gcagagttag cattcagaac agatccgtag agttgggctt
      781 ccaggcggta actgtggatc aggcgaacgt gaattttact gcaatgttgc tgtatgcagt
      841 ttttgatgat agcgaggaaa gcatcaagaa ggttgcgttc aagtttttag atgagcgcaa
      901 tttcatgcag gctttggttc gtactgtgga aggatccatt cgcgcatttg tggccagtaa
      961 aaaacaagca gaaattttgc ttttacgcag agagattgta gaagaggtaa aaattcagct
     1021 ggaccatacc ctcgaggagt ggggatacca tttgctcgat ctgcagatga acgatattac
     1081 cttcgacagt gaagtgatga actccatggc caaggtggta gcctctgcca acctgcgtgc
     1141 tgctgccgag aatgaaggtc aggcattgct tatcaccaaa accaaagcag ccgaggcaga
     1201 aggtaatgcc atcaagattg ccgctgaggc agaacgtgaa gcagctattc ttcgtggtca
     1261 ggccgtggcc cgtttccgtg aagaggtcgc caagggtatg tcgggtgctg ccaaggaaat
     1321 gcagcaggcc aacctcgatt cgagctttat tttgttctcc atgtggacag aagccatcaa
     1381 gaacttcgcc caggaaggca aggggaacct cattattctt gatggttcgg tagacggcat
     1441 gaaaaagacc ctcgcagagc tctccgcagc ctcgcgtttg ggcctctttg atcagcccga
     1501 cggaaaacac aaataatacc atttgatttg tcccgttttc cttgcgggct gccctatttt
     1561 ttctacattt gcagccccaa tttgcaggat atatgtcaac gggcattaaa tttttcgcag
     1621 gaagagctac ccaggatctg ggtcgcaagg ttgctgagag ttacggcgct aagcttggcg
     1681 aagtagctgt ttctactttt gccgatggcg aattccagcc ttctttcgaa gagacggtac
     1741 gaggtaaaga cgtgttcatc gttcagagca ccattccccc aaccgataac ctgttcgaac
     1801 tattactgct tgtagatgct gcccgccgtg cttctgcgcg taaggtggta gcggtgattc
     1861 cctacttcgg tttcgcccgt caggaccgca aagacaaacc ccgtgtttcc attggagcca
     1921 agcttgttgc caacctgctc actgcagccg gtgtagatcg catcatgacc atggatcttc
     1981 acgccgatca gattcagggc tttttcgagg ttccggttga tcatttgttc gcgagcaccg
     2041 tttttattcc ctacgtacag aacctcaatt tacccaatct catcatggcc gctcccgata
     2101 ccggaggaac caaacgtgcc aatgcttatg caaaatacct caactgcgat cttgccattt
     2161 gttacaagca acgcaagaag gccaatgaga tcgatagcat gacggtgatt ggtgatgtac
     2221 aaggcaaaga cgtggtgctt gtggatgaca tctgcgatac agcaggaaca ctcaccaaag
     2281 ctgcggatat gatgctggag cgtggcgcaa catctgtacg tgccatttgc acacatgccg
     2341 ttctttccgg aaacgcttat gagcgtatcg aaaaatcggg cattaccgag ctgattgtta
     2401 ccgacactat tccactgcgt cagcaatcac ccaagattcg tgttattacg gttgcaaatc
     2461 ttttcggaga tgtgatcaac cgtttgcaga attacgaatc catcagttca catttcatta
     2521 ttcagtaacc aattaaacac aaataaaaat gaagaaagta tcattgagcg gttcgccccg
     2581 tgcgaacgta gggaaaaaag atgcttccga cctgcgcaac aagggattga ttccttgcgt
     2641 gctttatggc ggtaatggtc aaacgcattt ctctattaac gaaatcgctt taaagaaagt
     2701 aatctggagt cccgacgtgt acaacgttga attggatatc gcaggtaaaa agaccaacgc
     2761 tattgttaag gatgttcagt ttcacaaagt atcagatcgc cttcagcata tcgattttct
     2821 tgaactggtt cccaacaaac ctgttaaact gaaacttcct gttcgtctaa ccggatcacc
     2881 tgagggtgtg aaaaaggggg gtaaactcgt tcagaattac cgtaaaatga atgtaatggg
     2941 cgctcctgaa aagttgcctg aagcgattat tgttgacgtt gaaaaacttg acatcggaca
     3001 aaacattcgt gttaagaaca tcagcatcga tggtcttcgt ttactcgaaa cagccgaatc
     3061 tgtggttgct gcagtagaaa ctacccgtaa cgtagccact gaagcaaaag atgccgcagc
     3121 tgcgaaaaaa taatagcaaa cagaaagctg catgaagtat ctgatcgtag gattaggcaa
     3181 catcggcagc gagtatgaag atacacgcca taacattggc ttttcggtac tggacgcctt
     3241 cgcaaaggcg tccagtgctg tttttaccag cgaccgtctg gcacatgttg ctgaggtaaa
     3301 attcaaagga cgcacacttg tgctcatcaa gcctactacc ttcatgaatc tctccggtaa
     3361 ggcggtcaat tactggttgc agcaggaaaa gatcagtccg gaaaacatgc ttgttattac
     3421 cgatgatctt gcactgccct tcgggaaaat cagaattcgt cctgcaggaa gcgacggggg
     3481 tcacaacgga ttaaaagaca ttcaggccgt attgggccac aacaattatc cgcgtctgcg
     3541 atttggtatt ggcgcagagt tttccaaggg aaaacaatcg gattatgttc tcgggaaatg
     3601 gggtgaagaa gaaaacaaaa cattaccaca acgcatcgaa ctgtgttgcg agatcattaa
     3661 aagtttcagc ac
//
LOCUS       DAOMYG010000324         3661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9076_length_3661_cov_11.509151, whole genome shotgun sequence.
ACCESSION   DAOMYG010000324 DAOMYG010000000
VERSION     DAOMYG010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9076_length_3661_cov_11.509151"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="PK610_10305"
     CDS             complement(<1..1167)
                     /locus_tag="PK610_10305"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ36370.1"
                     /translation="MSQRHIVVIGNGISGTTLARHVRKRSDDTITIISSESDYFYSRP
                     ALMYVYMGHMLQENTKPYEDRFWSENRINLRRAHVNRVDTVQKVLHLDDHSTMSYDVL
                     VIASGSHSNPGSWHGSHFEGVQGLVSLQDLSAMESNTQNCSRAVVVGGGLIGVEMVEM
                     LRSRGIHVTYLVREFKFWSSALRSEESDIVERHIIADHGVDLHTQTELKEILGDENGR
                     VRAVLTTKGDEIPCSFVGIAIGVKPNIAFLQESGIETGTGILVNECFETSAKDVYAIG
                     DCAEFRTALSGRTKIEQIWYTGRMQAETLAATLTGTTSVYRPVNFFNSAKLFDIEFQV
                     YSRNVKIPDTCRSVFWMNHHRRCAIRLFYDPQTLRFEGTVEDPGRNRMTQTLEGL"
     gene            1311..1934
                     /locus_tag="PK610_10310"
     CDS             1311..1934
                     /locus_tag="PK610_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36371.1"
                     /translation="MRLSRLLFPLFLILLCSCNGNLFRGSQYGNRQWVNAGPAYEAAA
                     DRRTPNTKASSEKAEHTPLPDSLFTTSTSPEENFHSENTACTTPPLSKKTFAPKLLIA
                     SEKRIRKKGFFSMAAGAIAGAAVVTGVIAAMFNGFAWGLLLLLGGILLAYLGIRLSLS
                     AKKDIDTLSSRSTIAWLNRPATIGFVMNIVVLILCGFVTLFLLMALI"
     gene            complement(1935..2393)
                     /locus_tag="PK610_10315"
     CDS             complement(1935..2393)
                     /locus_tag="PK610_10315"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HRJ36372.1"
                     /translation="MLQRIQTVYLFLATACLLLPFFFSIAKFDLGSASYLFTATGLMK
                     GGENLVAVPFQWIFLGLAGYTAAAIFMYGNRQLQMRMGRMNYLLLTALIITLYFVTDG
                     LPQKFEVPAAVIVSYGVGFFMPVAALAFVFLANRAIRSDENIIRSIDRLR"
     gene            complement(2402..>3661)
                     /locus_tag="PK610_10320"
     CDS             complement(2402..>3661)
                     /locus_tag="PK610_10320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HRJ36373.1"
                     /translation="GAQSAVYKNEEITMPRAATFLKSTDRNERELIYQLIVNRREKDT
                     VALDELFDLLRDLRHSVSLNAGYTNYRDYMFDALGRFDYSVSDCEDFHESIRTEILPI
                     TKGFMEERKERLQLDRLRPWDTEVDISGKPALKPFHGARELVDRSIRSMARVQPYFGE
                     CIRIMEQMGHLDLESKDGKAPGGYNYPLYEIGVPFIFMNAVDSQRDLVTMVHEGGHAV
                     HSFLTRDLELTAFKGTPSEVAELASMSMELLSMDAWDEFYTNAEELKRARKDQLEKIL
                     SILPWIATVDKFQHWLYVNPKHSSAERAAAWMQIQDEFSTHTVDYSGYEHSRKSAWQK
                     QLHIYEVPFYYIEYGMAQLGAIAIWRNYKKDPANAVKAYTEALKLGYTKSIPEIYETA
                     GIRFNFSRGYVKELADFVKSELEKLND"
BASE COUNT          983 a          894 c          780 g         1004 t
ORIGIN      
        1 gagtccttcc agggtctgag tcatgcgatt ccggccgggg tcttcaacag tgccttcgaa
       61 acggagtgtt tgcggatcgt agaacaaacg aatggcacag cggcggtgat gattcatcca
      121 gaatacagat ctacaggtat ccggaatttt tacgttgcgg ctgtaaacct gaaattcgat
      181 atcgaacagt tttgcagaat taaaaaagtt aaccggtcgg taaacactgg tggttcccgt
      241 aagcgtagca gcaagcgtct ctgcctgcat gcgaccggtg taccagattt gttcaatctt
      301 tgttcgacca ctcagcgcgg ttctgaattc cgcgcaatcg ccaatggcat aaacatcctt
      361 tgcagaagtc tcgaaacatt cgttcaccag tattcctgtc cccgtttcaa ttcctgattc
      421 ctgcagaaat gcaatattcg gttttacacc aatggcaatt cccacgaagc tgcagggtat
      481 ctcatctcct ttggtggtaa gtacagcacg tactcttccg ttttcatcac caagaatttc
      541 tttgagttct gtttgtgtat gcagatccac tccatggtcg gcgataatgt gccgctccac
      601 gatatccgat tcttcacttc gcaaggcact gctccagaat ttaaattcgc gcaccagata
      661 agtcacatga attccccgcg aacgcagcat ctccaccatt tccacaccga tcaatccgcc
      721 ccctactact actgccctgc tgcaattttg ggtattgctt tccatagcag aaagatcctg
      781 caaactcacc aatccctgca caccttcaaa atgcgaaccg tgccaggaac cgggattgga
      841 atgcgatccg cttgcgataa ccaatacatc ataactcatg gtactgtgat catccagatg
      901 caacactttt tggacagtat ccacacgatt aacatgcgct cgccgcagat tgatcctgtt
      961 ctccgaccaa aaacgatctt cgtaaggctt ggtattctcc tgaagcatat gccccatgta
     1021 cacatacatc agagcaggac gggaataaaa ataatcgctc tcggaagaaa tgatcgtgat
     1081 agtatcatcc gaacgcttcc gcacatgccg ggccagcgtt gtgccggaaa ttccattacc
     1141 aataacaaca atgtggcgct gcgacatagg gataaatgta ggaaaataaa aatgccaata
     1201 ggtaaagccg gagttatttt agatgaagaa ttaccaacag ctcaaagctg cgctcctgtt
     1261 gacctgcaat tgatttctta ttgtaatttc aagtaaattg tacacctgtt atgcgcttat
     1321 cccggctcct atttccactt ttccttattc tgttgtgttc ctgcaacgga aatctttttc
     1381 gtggttcgca atacggaaac cgtcagtggg tgaatgcagg tcctgcatat gaagctgctg
     1441 cagatcgtcg tacaccaaac acgaaagctt cctctgaaaa agcggaacac actcccctac
     1501 ccgattccct gtttaccacc tcaacaagtc ccgaggaaaa ttttcattca gaaaatacgg
     1561 catgtactac acctccgctt tcgaaaaaaa cattcgctcc caaattgctg attgcctccg
     1621 aaaaaagaat tcgcaaaaaa ggttttttct caatggcagc aggagcaatt gccggcgcag
     1681 cagtggtaac aggagtcatt gccgccatgt ttaatggttt tgcctgggga ctccttctcc
     1741 tacttggcgg aattctgttg gcttaccttg gaatccgact ctccttatct gcaaaaaaag
     1801 atatcgatac gttatcatca cgttcaacca ttgcatggct caaccgtcct gcaaccattg
     1861 gatttgtcat gaatattgtt gtgctgatcc tttgcggatt cgttacactc tttttgctga
     1921 tggcattgat ctgattaacg caagcgatca atagagcgaa taatgttttc gtcggaacga
     1981 atggctctgt tggcaagaaa aacaaaggct agtgcggcaa ccggcataaa aaatccgaca
     2041 ccatatgaaa caataactgc tgcaggaact tcgaattttt gtggcagtcc gtcggtcaca
     2101 aaatacaagg taatgatcaa agctgtgagc agaagataat tcatgcgtcc catgcgcatt
     2161 tgcaattgac gattaccgta cataaaaata gctgcagcgg tataacctgc cagtccgaga
     2221 aaaatccatt ggaaaggaac agctaccaga ttctctccac ccttcatcaa tccggttgcg
     2281 gtaaagaggt aagaagcgct gcccaaatcg aatttcgcaa ttgaaaaaaa gaaaggaagc
     2341 agcagacaag cagttgccag aaagagataa acggtttgaa ttcgttgtaa catgacagca
     2401 attaatcgtt gagtttttcc aattcgcttt ttacaaaatc agcaagttct ttcacgtatc
     2461 cgcgcgaaaa attgaatcgg ataccggctg tttcgtaaat ctcgggaata cttttggtat
     2521 agcccagttt gagtgcttcg gtataggctt ttactgcgtt tgcaggatct tttttgtagt
     2581 ttctccagat agcaatggca cctagttgag ccattccgta ttcgatgtaa tagaagggta
     2641 cttcgtaaat atgcaattgc ttttgccagg cgcttttgcg ggaatgttcg taaccggaat
     2701 aatcaacagt atgcgtcgaa aactcatcct gtatctgcat ccatgccgct gctctttctg
     2761 cgctgctgtg ttttggattt acatataacc aatgctgaaa tttatcgact gttgcgatcc
     2821 atggtaaaat gctcagaatt ttttcgagtt gatctttgcg tgcccgtttc agttcttcag
     2881 cattggtata aaattcatcc catgcatcca tactcagcaa ttccatactc atggatgcca
     2941 gttctgcaac ttcgctggga gttcctttaa acgctgtaag ctcaagatcg cgggtgagaa
     3001 aggaatgtac cgcatgtccg ccctcgtgca ccatggtaac caggtcacgt tgcgaatcca
     3061 ctgcattcat aaaaataaaa ggcacaccga tttcgtagag cggataattg tatccaccgg
     3121 gtgcttttcc atctttcgat tcgagatcga gatgtcccat ttgttccatg atgcggatgc
     3181 actcaccgaa ataaggctgc acccttgcca tggaacggat gctacggtcc accagctcgc
     3241 gtgcaccgtg aaaaggtttg agcgccggtt ttccgctaat atcaacctct gtatcccagg
     3301 gacgcaatcg atcgagttga agacgttctt tccgctcttc catgaaccct ttggtaatgg
     3361 gcaggatttc ggtacgtatg ctctcatgaa aatcttcgca atcgctcacc gaataatcga
     3421 aacgaccgag cgcatcgaac atgtaatcgc ggtaatttgt atatcctgca ttgagactta
     3481 ccgaatgccg gagatcgcgc aacagatcga acaattcatc caatgctacg gtatcttttt
     3541 ccctgcggtt tacgatgagc tgataaatca attcccgctc attgcgatcg gtcgatttaa
     3601 gaaatgtagc tgcgcgtggc atggtgattt cttcgttctt gtacaccgca ctttgtgcgc
     3661 c
//
LOCUS       DAOMYG010000325         3655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9101_length_3655_cov_10.206944, whole genome shotgun sequence.
ACCESSION   DAOMYG010000325 DAOMYG010000000
VERSION     DAOMYG010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9101_length_3655_cov_10.206944"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PK610_10325"
     CDS             <1..837
                     /locus_tag="PK610_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ36374.1"
                     /translation="QDQLLTKEGALKSEFNDKIHLQRHPWLKTDYIGYILKEDQPVFS
                     NSPTRIKQVRKAINYSINRKEMITHLRNGIGQPAESGFTPMGMQSFKYTSIIGYNFDL
                     DKARALLFEGGITKPREVEPIKLIATMEYKQICEYIKMNIEELGLRVDLELVLPNVQK
                     QMIAGYQTNMFRKSWTADYPDGINFLQLFYSKNESPRGPNYTHFRNGPFDVNFELALS
                     ETNDAKRYELYHKMEEIIMEEAPIIPLYYDEVVRFVQNYVTDLGFNAMNKLDLKRVRI
                     NK"
     gene            841..1920
                     /gene="aroC"
                     /locus_tag="PK610_10330"
     CDS             841..1920
                     /gene="aroC"
                     /locus_tag="PK610_10330"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRJ36375.1"
                     /translation="MPGNQFGQLLSLTSFGESHGEAIGGILEGMPAGISIDLAFIQNE
                     LDRRKPGQSAIVSQRKEPDSINILSGIFEGKTTGTPIGFIIANEDQRSKDYSHNEDVF
                     RPSHADYTYQAKYGMRDHRGGGRSSARETAVRVAAGAFAKILLSHYNISIHAWVNSVG
                     AISIEKKHTELDLSQIEKNSVRCADPVIAEKMIAYIDSIRKQGDSVGGTVRCVIKNCP
                     AGLGEPVFDRLHADLGKAMLSINAVKGFEYGSGFAGTLLKGSEHNDAFVNENEKIRTA
                     TNFSGGIQGGISNGEEIYFTVAFKPVATIMMEQDTVNASGEPAVIKGKGRHDPCVLPR
                     AVPIVEAMSALVLADHLLRHRSSQI"
     gene            1931..3199
                     /locus_tag="PK610_10335"
     CDS             1931..3199
                     /locus_tag="PK610_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRJ36376.1"
                     /translation="MKIALHWKILIGVILGLIWGIVSVEYDYSVFTSNWIKPFGTIFM
                     NMLKLVAVPLVMVSLIDGIGSLGNVSGLSRMGGKTIGIYILTTMLAISIGLVMVNLIR
                     PGDFVSAEKKTELQEKYSSDAKDKIETAAKEKEKGPLNMLENIVPDNIVRSMSDNKLM
                     LQVIFFALLFGIAVISLPADKTKPVRDFIRSFNEVMLKLVELIMAFAPYGVFALLASM
                     NADLAMLSALGVYCLAVLLGLGLMVTVVYPLMLKLFSNQPIVPFFRKILPAQLLAFST
                     SSSAAALPVNMKICEEELGMKEETTGFVLPLGATINMDGTSLYQAVAAIFIAQTYGME
                     LGLTEQLGIVLTATLASIGAAPVPGAGMVMLVIILQSAGLNAEGLALIIGVDRILDMF
                     RTTVNITSDATVAAIIDHQERKREEGIHQA"
     gene            3331..>3655
                     /locus_tag="PK610_10340"
     CDS             3331..>3655
                     /locus_tag="PK610_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36377.1"
                     /translation="MFGKKNTDPSASKTKKPLWRRILKWTGISFLLLFIFALAAPFLF
                     KDKIFALIKEQINNNLHGKFECEDYSLTLISSFPNFTMELKEVSLTGVDDFEGVKLLD
                     AENLVL"
BASE COUNT         1114 a          781 c          803 g          957 t
ORIGIN      
        1 caggatcagc tattgaccaa agaaggagcg cttaaatcgg aattcaacga caagattcac
       61 ctgcagcgtc acccctggtt aaagacggat tacatcggat atattcttaa agaagatcaa
      121 cccgtttttt caaatagtcc gacaaggatc aaacaagtcc gcaaagcgat caattactcc
      181 atcaaccgca aagaaatgat cactcacctg cgcaacggaa ttggtcagcc tgcagagagc
      241 ggatttacgc caatgggtat gcagagcttt aaatacacca gtatcatcgg ttataatttc
      301 gatctggata aagcacgtgc acttttgttt gaaggtggaa ttaccaaacc acgtgaagtg
      361 gagcccatca agcttattgc tacaatggaa tacaaacaga tttgcgaata catcaaaatg
      421 aacattgagg aacttggctt aagggtagat cttgaattgg tattgcccaa tgtgcagaaa
      481 caaatgattg cgggttacca gacgaacatg ttcagaaaat catggaccgc agattatcca
      541 gacggcatca attttcttca attgttctat agcaaaaatg aatcaccaag aggacccaat
      601 tacacgcatt tcagaaatgg tccgtttgat gtgaatttcg aactcgcatt atccgaaacc
      661 aacgatgcca agcgttatga gctttaccac aaaatggaag agatcattat ggaagaagca
      721 ccgatcattc cactatatta tgacgaggtg gttcgattcg tacagaatta cgttacagat
      781 cttggtttta atgcaatgaa caaactcgac ttgaaaagag tccgaatcaa taaataaact
      841 atgccgggaa atcaattcgg tcagctgtta agtctcactt ccttcggaga atcgcatggc
      901 gaagctattg gcggaattct ggagggaatg ccggccggaa tatcaatcga tcttgctttc
      961 atccaaaacg aactcgatcg cagaaaaccc ggacaatccg caatcgtttc ccaacgcaaa
     1021 gagccggaca gcatcaacat tctgtcggga atattcgaag gaaaaacaac gggaacaccg
     1081 atcggattta tcattgccaa cgaagatcag cgtagtaaag attattccca taacgaagat
     1141 gtctttcgtc cttcgcatgc cgattacacc taccaggcca aatacggcat gcgcgatcat
     1201 cgcggaggag gtcgttcctc agcaagagaa acagcagttc gtgtagctgc aggtgctttt
     1261 gcaaaaattc ttttatcgca ctacaacatt tctatccacg catgggtcaa cagcgttgga
     1321 gccatcagca ttgaaaaaaa acatacagaa ctcgatctct cacaaatcga aaaaaacagt
     1381 gtgcgttgtg cagatccggt aatagcagaa aaaatgattg cgtacatcga ttccattcgc
     1441 aaacagggag actcagttgg cggaacggtt cgttgtgtga tcaagaattg tcctgccgga
     1501 ttaggcgagc ccgtttttga ccgactgcat gccgatcttg gcaaagcgat gctaagtatc
     1561 aatgcagtga aaggttttga atacggatca ggttttgcgg gcaccttgtt aaaaggatca
     1621 gagcacaatg atgcattcgt aaatgaaaat gaaaaaatcc gcacggccac caatttttca
     1681 ggtggaattc aaggcggaat ttcgaatgga gaagaaattt atttcacggt tgcatttaaa
     1741 cctgttgcaa ccatcatgat ggaacaggac accgtaaatg catccggaga acctgcagtg
     1801 ataaaaggta aaggaagaca cgatccttgt gttttgcctc gcgctgttcc aatagtagaa
     1861 gccatgagcg cactcgttct ggccgatcat cttcttcgtc accgcagttc acaaatctga
     1921 aaacaacact atgaaaatcg ctcttcactg gaaaatactt atcggtgtaa ttcttggact
     1981 tatctggggt attgtttctg tagaatacga ttattctgtt tttacatcca attggatcaa
     2041 acctttcgga accatcttca tgaatatgct gaaactcgtt gccgttcctc tggtaatggt
     2101 ttcgctgatt gatggaattg gtagtctggg aaatgtttcg ggactttcac gcatgggagg
     2161 taaaacgatc ggaatctata ttctaacaac catgctcgcc atcagtatag gactggtaat
     2221 ggttaatctg attcgtccgg gcgactttgt ttctgcggag aaaaaaacag agttacagga
     2281 aaaatacagc agcgacgcaa aagacaaaat tgaaacggct gcaaaggaaa aagaaaaagg
     2341 tccgctcaat atgctcgaga atattgttcc cgacaacatt gtgcgctcta tgagtgataa
     2401 taaattgatg ctacaggtca tcttctttgc acttcttttt ggaattgcag tcatcagtct
     2461 gccggcagat aaaacgaagc cggtccgcga tttcatccga tccttcaatg aggtgatgct
     2521 aaaactggtt gaactcatta tggcatttgc gccttatgga gtatttgcat tgctggcatc
     2581 gatgaatgca gatcttgcca tgctaagtgc attgggtgtg tattgcctgg cggtgcttct
     2641 cggactcgga ttaatggtta cagtggtgta tcccctgatg ttgaaactct tctccaatca
     2701 gccgattgtt ccctttttca ggaaaatact tccagcacaa ttattggcat tttccaccag
     2761 ctcgagtgca gcagcattgc ctgttaacat gaaaatctgt gaagaagaac tcggaatgaa
     2821 ggaagaaacc acaggatttg tattacccct tggagccacc atcaacatgg acggcaccag
     2881 tctgtaccag gcagtagctg ctatttttat tgcccaaacc tacggaatgg aactggggct
     2941 tacagagcaa ttgggaattg tattaaccgc caccctcgcc tccattggcg cagctcctgt
     3001 tcccggagca gggatggtaa tgctggtaat catattgcag tcggccggac ttaatgctga
     3061 ggggctggca ttgatcatcg gggtagatcg tatcctcgac atgttcagaa caaccgttaa
     3121 catcacttcc gacgcaacag tagcagcgat cattgatcat caggagcgta agcgcgaaga
     3181 aggaatccac caggcttagc ataaagggag atcacagtaa ttacggcaac agcacttcag
     3241 tttcagctat aatattaaca tctgttaatt acaggctgaa aatgatcctt ttagcattaa
     3301 atttgtactg catctaaaaa cccttgaaat atgtttggta aaaagaacac agaccctagt
     3361 gcttcaaaaa caaaaaagcc actttggaga cgcattttaa aatggacagg aatcagcttt
     3421 ttactgctgt ttattttcgc actcgccgct ccgtttttat tcaaggacaa gatttttgca
     3481 ttgatcaaag aacagatcaa caataatctt catggaaaat ttgaatgtga ggattacagc
     3541 ctcacattga tcagctcctt tcctaacttc accatggaac tgaaagaagt ttcactcaca
     3601 ggtgttgatg attttgaagg tgtaaaactt ctggatgcgg agaacctcgt tttaa
//
LOCUS       DAOMYG010000326         3637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9172_length_3637_cov_8.164712, whole genome shotgun sequence.
ACCESSION   DAOMYG010000326 DAOMYG010000000
VERSION     DAOMYG010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9172_length_3637_cov_8.164712"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..459)
                     /locus_tag="PK610_10345"
     CDS             complement(40..459)
                     /locus_tag="PK610_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HRJ36378.1"
                     /translation="MLKQVPLEQLNAMNRNTIMEVLGIEYTELSPTTISGKMPVDHRT
                     HQPVGLLHGGATAVLAETLGSMGSTLLIDTKEFAVTGVEVNANHLKGVKNGFVHATAT
                     ILHAGRTTHVWNIEVKNDEQNLVAVCRLTVVVIPLKK"
     gene            637..2433
                     /locus_tag="PK610_10350"
     CDS             637..2433
                     /locus_tag="PK610_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRJ36379.1"
                     /translation="MTVFAQLGLKEEIVKAVSDLGFENPTLIQQQAIPLLLAEPTDLV
                     GLAQTGTGKTAAFGLPMIHHIDFSDRTTQALVICPTRELCLQITKDLQSYSKYIPQAN
                     VVAIYGGASIDNQARDIRRGAQIVVATPGRLMDMIDRRKVDISSVQFVVLDEADEMLN
                     MGFKEDIDIILKSVPSDRSTWLFSATMPREVASIAKNYMKNPQEITVGKQNSSNENIE
                     HQYFMIREKDRYFALKRIIDFYPEVFGIIFCRTKRETQEVSDKLIKDGYNCEAIHGDL
                     SQGQRDSVMKKFRERTLQLLCATDVAARGIDVSNITHVINYNLPDDIENYTHRSGRTA
                     RAGKSGFSLVLVNTKERNRVRDIERVIKKDFVQRMIPNGKEICEKQLFALIERMTKVE
                     VNEKGIAPYLDRINESLASLSKEDIIKRFVSAEFNRFIDYYKHSEDLNVTMKNDRNDR
                     GERRERGDRNDMQSLFFNVGDMDGVNKGMLVRLICDNAGIAGNQIGRIDIRREFTFCD
                     VDPSIAKQIMDGLNGLVVEETGRKLRVNPADRPSGGGDGGRDRNKGGGGKSYGGGGGR
                     KSYGGGEKKSYGGDKKSFGGPNRKKSFSKRDY"
     gene            complement(2481..3335)
                     /locus_tag="PK610_10355"
     CDS             complement(2481..3335)
                     /locus_tag="PK610_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36380.1"
                     /translation="MRKLILLFLVCACQAEQSPKEPDPINSIEENKPAPPGLSSIIAI
                     TDTRKPDAISQAVLQYRINTSMNSPLHCDNDLVILKAFAIRAAHDINQTGFLDTMIRD
                     DGRISPGDERKLNRLGYLVNYVDGEPYLSASPGYLLESLRPCLSAGMIRFLEEFEKEA
                     RQTATQGEQLQISVDELLRRVLFWEDFMNEYHNFVLKEEVKTTYHFFLSLYMTGTGTS
                     RAFDARTLHFNPEFRMSYERFMKSNANSPTAKFISDYYLVLKQNNFYYSSEVENYYSS
                     VQSPYWGK"
     gene            complement(3332..>3637)
                     /locus_tag="PK610_10360"
     CDS             complement(3332..>3637)
                     /locus_tag="PK610_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36381.1"
                     /translation="SDGRTLLSVIEPEKIKTEDQQMEMTRERMSTFFAETGFSNPPYT
                     LNDPIKSGITGDKELWKEIENDSTAWCFEFSLSKGGGVEVISYSRLRDTILVYDSSQ"
BASE COUNT         1011 a          767 c          808 g         1051 t
ORIGIN      
        1 taattcctga attttctgat gtaggggagg agccatggat tattttttga gtggaattac
       61 taccacagtt aaacgacaaa cagcaactag attttgttca tcgtttttaa cctcgatatt
      121 ccatacgtgt gtggttcttc ctgcatgtaa gattgtggct gttgcatgca caaaaccatt
      181 ttttacacct ttcagatgat tggcattcac ctcaacacct gtaactgcaa actcttttgt
      241 gtcgatcaaa agtgtcgacc ccatacttcc taaagtctct gcgagcactg ccgtcgcgcc
      301 tccgtgcaaa agtccaacgg gttgatgggt acggtgatcg actggcattt taccactgat
      361 agtggtaggg ctaagttcag tgtattcgat tcccagaacc tccataatgg tattccggtt
      421 catcgcattt aattgttcaa gggggacttg tttcagcatc tgcgaatgta gcttttcctt
      481 tgctttaaac ttacttgtat cgtattaagc ctaaaggggg ttaagaacaa ttggaggaga
      541 taggactcga ctgttttcac ctaagttgcg gtgtttccga tatttatcct tacctttgcg
      601 ccagttctta aattattttc taaagttaaa gtcagaatga cagtatttgc ccaattgggc
      661 ctgaaggaag agatcgtaaa agccgtgtcc gacctgggtt ttgagaaccc aacacttatc
      721 cagcaacaag caatcccttt acttcttgcc gagcctaccg acctagtcgg gctcgctcaa
      781 accggaaccg gtaagaccgc cgcatttggt ctgccgatga tccaccacat tgatttcagt
      841 gaccgcacta cgcaggcact ggtcatatgt cctacccgtg aattgtgtct gcagatcacc
      901 aaagatcttc agagctattc aaagtatatt ccgcaagcga atgttgtcgc tatttacgga
      961 ggtgcttcta tcgataatca ggcccgcgat attcgtcgtg gtgcacagat cgtagttgca
     1021 acaccggggc gactcatgga catgattgat cgccgtaagg ttgatatttc gtctgtacag
     1081 tttgttgtgc tcgacgaggc ggatgaaatg ttgaacatgg ggttcaagga agatattgat
     1141 atcatcctga aaagcgtgcc ttctgatcgc agcacctggc tgttctctgc aacaatgcca
     1201 cgtgaggttg cttcgatcgc taaaaactac atgaaaaatc cgcaggaaat taccgttgga
     1261 aaacaaaact cttccaacga gaatatcgag caccagtatt tcatgatccg tgaaaaagat
     1321 cgctatttcg cgcttaagcg tattatcgat ttttaccctg aggttttcgg aattattttc
     1381 tgtcgcacca aacgcgaaac acaggaagtt tcagataaac ttatcaagga cggatacaac
     1441 tgtgaagcta ttcatggtga cctttcacaa gggcagcgtg atagtgtaat gaagaaattc
     1501 cgtgagcgta ccttgcaatt attatgtgca accgatgtgg ctgcgcgtgg tattgatgta
     1561 agtaatatta cacacgtaat caattacaat cttcccgacg atattgaaaa ctacacccat
     1621 cgctcaggtc gtactgcgcg tgcaggaaaa tcaggttttt ctctggtgtt ggtaaatacg
     1681 aaggaacgta accgtgttcg cgatatcgaa cgcgtgatta agaaagattt cgttcagcga
     1741 atgattccga atggaaaaga gatttgcgaa aaacaacttt ttgcactcat tgaacgcatg
     1801 accaaagttg aagtcaacga aaaaggaatt gctccttacc tcgatcgaat caacgaatca
     1861 ttggcgtcgc tttccaaaga agacattatc aaacgttttg tgtctgccga atttaatcgc
     1921 tttatcgatt attacaaaca ttctgaagac ctcaatgtaa caatgaaaaa cgatcgtaac
     1981 gatcgtggag aacgccgtga acgtggcgat cgcaatgata tgcagagtct gttcttcaat
     2041 gttggagata tggatggcgt aaacaaagga atgctggttc gcttgatttg tgataatgca
     2101 ggtattgcag gaaaccaaat tggtcgtatc gatattcgtc gcgaattcac tttctgtgat
     2161 gttgaccctt caatcgctaa gcaaatcatg gatggattga acggactcgt ggttgaagaa
     2221 accggtcgca aattacgcgt caatcctgct gatcgtcctt ccggcggcgg tgatggcgga
     2281 agagaccgta acaaaggcgg cggaggtaaa tcctatggcg gcggtggcgg tcgtaaatct
     2341 tacggcggtg gtgaaaagaa atcctacggc ggagataaaa aatcgttcgg tggaccaaac
     2401 cgtaaaaaat cattcagcaa gcgcgactat taatcgcgtc tgaaaaaata gtttagccgg
     2461 gtctttttga tccggctttt ttattttccc caatatggcg attgtacaga ggaataatag
     2521 ttttctactt cgcttgaata gtagaaattg ttttgcttca gaacaaggta gtaatcacta
     2581 ataaatttgg ctgtgggtga atttgcgttc gacttcatga aacgctcata actcattctg
     2641 aattccggat taaaatgcaa tgttcttgca tcaaaagctc tgcttgttcc ggttccggtc
     2701 atatataaac tcagaaaaaa gtgataggtg gttttcacct cttccttcaa aacaaaattg
     2761 tgatactcat tcataaaatc ctcccagaat aaaactcttc gtaataactc gtcaacactg
     2821 atctgcaatt gttctccttg cgtagcagtt tgtcgtgctt ctttttcgaa ttcttcaagg
     2881 aaacgaatca tgcctgcaga taaacaagga cgtaaactct cgagcaggta gcctggactt
     2941 gcactcaaat agggttcacc gtcaacataa ttaaccaggt atccaaggcg gttcaacttg
     3001 cgttcatctc ccggactgat ccttccgtca tcgcgaatca tagtatcgag aaaaccggtt
     3061 tggttgatgt catgcgctgc tcgaattgca aatgctttca gaataacaag atcattgtcg
     3121 caatgcaaag gagaattcat actggtgtta atacggtatt gcagtaccgc ttgcgaaatg
     3181 gcatcaggtt tcctggtgtc ggtgatggca atgattgaac tcaggcctgg aggagccggc
     3241 ttgttttcct ctattgaatt aatcggatca ggttctttag gcgattgttc ggcctgacaa
     3301 gcgcaaacaa ggaaaagcag aattagtttt ctcattgact ggaatcgtaa accaaaatgg
     3361 tatcgcgcaa tctggaatag gaaataactt ccacaccacc gcctttactg agtgaaaact
     3421 cgaaacacca agctgtgctg tcattttcaa tttctttcca aagttctttg tctccggtga
     3481 tgccggattt aatggggtca tttaaagtat aaggtggatt ggaaaaaccg gtttcagcaa
     3541 aaaaggtact catgcgctct ctggtcattt ccatttgctg atcttctgtc tttatttttt
     3601 caggttcaat aacggataat aaagttctgc catcact
//
LOCUS       DAOMYG010000327         3628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9200_length_3628_cov_8.187238, whole genome shotgun sequence.
ACCESSION   DAOMYG010000327 DAOMYG010000000
VERSION     DAOMYG010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9200_length_3628_cov_8.187238"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PK610_10365"
     CDS             <1..678
                     /locus_tag="PK610_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020081532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ36382.1"
                     /translation="PAYNDVIQFWKNSATGYTPTLIVSYGSVNGEYYWYQHTNVWEKE
                     RLLRFTPRAVIDARSRHRTMVPEEEYENGHILASKTCKALADQGVKVNMGSHGQIQGI
                     GAHWEIWMLVQGGMSPHEALCAATYNGAHYIGIEDQVGSLAKGKLADLLVLDKDPLTD
                     IRNTESISLVMVNGRLYDAEQMNEIGNYDKKRSRFFWELPNSNQSYKVHSETNGFTRP
                     QCVCGKH"
     gene            675..>3628
                     /locus_tag="PK610_10370"
     CDS             675..>3628
                     /locus_tag="PK610_10370"
                     /inference="COORDINATES: protein motif:HMM:NF019174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRJ36383.1"
                     /translation="MKQVNQHIVFLILFLQIGLVRAQNYADPRRFYFQQYSVENGLPQ
                     SQVNSIFEDSRGFLWFATQGGGVARFDGSKFLQYEEKEGLAGQIATCITEDDKGRMWF
                     GTSWGGLTCFDGKNFKIFSRENGFTTNEVKSLIFDPEKSRVWVGLHWCLAYIENDVVV
                     RHPDFNQLDVVSMAITNEGELAVATSNGLFLVGEDNKARLVPSTLDQAIRHVTVDSVS
                     LWITSETGLFRLRTSDMQIMELPSVITNCQDLDFSSLSSTHIDASGQLWIASESDGLF
                     LYKGDKIFHFTPENGLTDKNVQCVFSDRSGQLWVGTRGSGVLRFSDFAFSNYFGLQGL
                     GLPDIFGITTDMDGVLWVGAMSEGLFRFDGKAIRKYSQKDGLPSPVIRQIVVARNGDL
                     LLATKEGFCIFDGTRFKVFTEDQGLSSRNIRCLTQTKDGKIWLGTAGGGLVMMEGNKL
                     EIFTVTNGLPHDYVHSLLEDSKGNLWIGTGAGLTKYADGIFSSYDGKEGLCNSYVGSM
                     AEDRFGNIWVGTDKCISRFNGRRFTNYDVSDGLGSNTIYLLATDHSGSLIVGTNKGFD
                     VVSFSAYGQISSIKNYTHNDGFLGIECNNKAVHVDANGVIWFGTVKGLIRFDPNEEPE
                     EKVRPYIHISDVRLFHDTKWKKDQNYKAFDWFDLPIEPVFRHDQNSINFEFIGICQRY
                     PETLEYSYMLEGFDKKWSPLSSSTLATYSNLAPGEYLFRVKARSKSGLWRIPEARYSF
                     SIEPAFYQTWWFFLLLLVSLVYGVYYFNTFMQRNVLRQNQLLEEKVNVRTREILKQKE
                     EKELLLKEIHHRVKNNLQVIVSLLNIHSDYVKDPESIALLEDSKSRIKSMALIHEKLY
                     EGKNFSKVNIGEYLDTLVKELVETYGTDTRIQIDRSINADNFGFDTIIPLGLLLNEIV
                     SNSLKYAFPGKEEGVLYFHLKGEGKKFELLIGDNGIGIDRKVFFESNKTLGVDLIRIL
                     TEQLNGEIELLEG"
BASE COUNT         1042 a          619 c          877 g         1090 t
ORIGIN      
        1 cctgcataca acgatgtcat tcagttttgg aaaaattctg ctactggtta cacgcctaca
       61 ttgatcgttt catacggctc agtaaacgga gaatattatt ggtaccagca cactaatgtg
      121 tgggaaaagg aacgtttatt gcgtttcact ccacgggcag tgattgatgc acgttcacgt
      181 catcgcacaa tggttccgga agaagaatac gaaaacggac atattctggc atctaaaaca
      241 tgtaaggcat tagctgatca gggtgtaaaa gtaaatatgg gttcacatgg tcagatccag
      301 ggaattggtg cacactggga aatctggatg cttgttcaag gcggaatgtc gccgcatgaa
      361 gccttatgtg ccgcaactta taacggagct cattatatcg gaattgaaga ccaggttggc
      421 tcacttgcga agggaaaact tgcagattta ctagtgctgg ataaggaccc gcttacagat
      481 atccgtaata cggaatctat ttcattggtg atggtaaacg gcaggttgta cgatgctgag
      541 cagatgaatg aaattgggaa ttatgataaa aagcgttccc gttttttctg ggagctgcca
      601 aatagtaatc aatcctacaa ggtgcattct gaaacaaacg gatttacacg tccccaatgt
      661 gtttgcggta aacattgaaa caggtaaatc aacatatcgt attcctgatt ctgtttttac
      721 agattggttt agtgcgtgca caaaactatg cagatccaag aaggttttat ttccaacagt
      781 actctgtaga aaatggattg ccacagtcgc aggttaattc catttttgaa gattcacgag
      841 gatttctatg gtttgccact cagggcggag gtgtcgcacg atttgatggc tctaagtttt
      901 tgcaatacga agaaaaggaa ggtttagcag gacaaatcgc gacctgtatc actgaagatg
      961 acaagggaag aatgtggttt ggtacttcat ggggtggttt gacatgtttt gatggaaaga
     1021 atttcaaaat tttcagcaga gaaaacggct tcacaaccaa tgaagtgaaa agtctgattt
     1081 tcgatccgga aaagtcgcgc gtttgggttg gtttacattg gtgtctggct tacatcgaaa
     1141 atgatgttgt tgttcgtcac cctgatttta atcaactgga tgtagtttca atggcaatta
     1201 ccaatgaagg tgagttggcg gttgcaactt ccaacgggct gtttttggtt ggagaggata
     1261 ataaagcccg tttggttcct tccacgctcg atcaggcaat acgacatgta acggttgatt
     1321 ctgtttctct ttggattact tctgaaaccg gattgttcag actccgaaca tcagatatgc
     1381 agattatgga attaccatcg gtcattacta actgtcagga tctggatttt tcctctttat
     1441 ctagcactca tattgatgca tccggtcaat tatggattgc ttctgagtcg gacggattat
     1501 tcttatataa aggcgacaag atttttcatt tcacgcctga aaatggattg actgacaaaa
     1561 atgttcaatg tgttttttcg gatcgttccg gacaattatg ggttggtaca cgcggttcag
     1621 gcgttttacg attctccgat tttgcctttt caaattattt tggtttacag ggcttgggct
     1681 tgcctgatat ttttggcata acaaccgata tggatggggt attgtgggta ggagcaatgt
     1741 cggaaggact ttttcgtttt gatgggaaag caatccggaa atacagtcag aaagacgggt
     1801 tgccttctcc tgttattcgg caaatagttg tagccaggaa tggcgatttg ctattggcaa
     1861 ccaaggaagg tttttgcatt ttcgatggaa cacgttttaa agtttttacg gaggatcagg
     1921 gattatccag ccgtaatatc cggtgtttga cacagacaaa agacggaaag atctggcttg
     1981 gaactgcagg aggtggattg gtgatgatgg aaggaaacaa actggaaata tttacggtta
     2041 caaatggttt acctcatgat tatgtgcatt ctttactcga agattcaaaa ggaaatttgt
     2101 ggatcggaac aggagcagga ttgacaaaat atgcagatgg aattttttct tcttacgatg
     2161 ggaaggaagg actgtgtaac agttatgtgg gaagcatggc ggaagaccgt tttggaaata
     2221 tctgggtagg aacagataaa tgtatttcca ggttcaacgg ccgacgtttc accaattatg
     2281 atgtgtcgga tggattaggt tcgaatacaa tctatcttct ggcgacggat cattcaggga
     2341 gtttaattgt gggaacgaac aaagggtttg acgtagtgtc gttttctgct tacggtcaga
     2401 tttcttctat aaagaactac acccataacg atgggttttt gggaattgaa tgcaacaata
     2461 aggccgtgca cgttgacgca aatggagtaa tatggttcgg tacagtaaaa ggattgattc
     2521 gttttgatcc gaatgaagaa ccggaggaaa aggtgcgtcc ttatattcat atttcagacg
     2581 taagactgtt tcacgacaca aaatggaaaa aggatcagaa ttataaggca tttgattggt
     2641 tcgatcttcc cattgagccg gtatttcgtc acgatcagaa cagcattaat tttgagttta
     2701 ttggaatctg tcaacgttat cctgaaacat tggaatattc ctatatgttg gaaggatttg
     2761 ataaaaagtg gtcgccatta agcagctcca cattggcaac ctattccaat cttgcgcccg
     2821 gtgaatattt gtttcgtgtt aaagccagaa gtaaatcagg tttatggcgc ataccggaag
     2881 cacgttattc gttctccatt gaacctgcat tttatcagac ctggtggttc tttttattgt
     2941 tgctcgtttc gcttgtatat ggcgtatatt acttcaatac gtttatgcag cgaaatgtgt
     3001 tgcgtcagaa tcaattgctg gaggagaaag taaatgtgcg tacacgcgaa attcttaagc
     3061 aaaaagaaga aaaggaatta ttgcttaagg agattcacca tcgtgtaaag aataatctgc
     3121 aggtaattgt ttcactgctt aatatccatt cggactatgt aaaagatccc gaatcaattg
     3181 cattgctgga ggattccaag agtcggatta agagcatggc ccttattcat gaaaagttat
     3241 atgagggtaa gaatttttcc aaagtcaata ttggggaata tctggataca ctggtgaaag
     3301 agcttgtgga gacctatggt accgatacac gcatacaaat cgacagatcc atcaatgccg
     3361 ataattttgg attcgacacc atcattccgc ttgggttatt gttgaacgag attgtttcca
     3421 attccctgaa gtatgcattt ccgggtaaag aagaaggtgt tttgtatttt catttaaagg
     3481 gtgaaggcaa gaaatttgaa ttgcttattg gggataatgg aattggtatc gaccgcaaag
     3541 tcttttttga atcgaataaa accctgggag tggacctcat ccggatttta acggagcaac
     3601 tcaatggcga aattgagctc cttgaagg
//
LOCUS       DAOMYG010000328         3620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9226_length_3620_cov_10.435344, whole genome shotgun sequence.
ACCESSION   DAOMYG010000328 DAOMYG010000000
VERSION     DAOMYG010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9226_length_3620_cov_10.435344"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PK610_10375"
     CDS             <1..549
                     /locus_tag="PK610_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HRJ36384.1"
                     /translation="AEWALNWISKGSFAERLVAFAAVEGIFFSGSFCSIFWLKKRGLM
                     PGLSFSNELISRDEGLHCDFACLLYSQHLVNKLDKKVVEQIITEAVAIEKEFVTDALP
                     VKLIGMNADLMSQYIEFVADRLLLELGNPKVYNVTNPFDFMDMINLQGKTNFFEKRVG
                     EYQKAGVMSTKEENKFSLDEEF"
     gene            601..3000
                     /locus_tag="PK610_10380"
     CDS             601..3000
                     /locus_tag="PK610_10380"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HRJ36385.1"
                     /translation="MFVIKRDGRKESVQFDKITARIKKLCYGLNPIVDPVSVAIKVVE
                     GIYDGVTTSELDNLAAEVAATNTIKHPDYALLASRIAVSNLHKNTKKSFSLTMKDLYD
                     YVDPRTGERASLLADDVAEIIEKNAELLDSAIIYDRDFRFDFFGFKTLERSYLLKLHS
                     KVAERPQHMFMRVAIGIHKEDLKNAIETYHLMSEGWFTHATPTLFNAGTPKPQMSSCF
                     LLSMKKDSITGIYDTLTQCAKISQSAGGIGLSIHDIRATGSYIKGTNGTSNGIVPMLR
                     VFNDTARYVDQGGGKRKGSFAIYIEPWHADIFDFLDLKKNHGKEEQRARDLFYAMWIP
                     DLFMQRVEDNGEWTLMCPNECPGLSDTYGKNFEDLYTKYEREGKGRKTIKAQDLWFKI
                     LESQIETGTPYMLYKDACNEKSNQKNLGTIKSSNLCTEIMEYTSPDEVAVCNLASIAL
                     PKFVKDGEFDHQRLFDITYHITKNLNKIIDYNFYPVPEARRSNMRHRPIGIGVQGLAD
                     AFILMRQGFDSDEARKLNSEIFETIYYAALTSSKDLAKEFGPYESYEGSPISQGIFQH
                     DMWNVTPSPRWEWDVLREEIKQHGVRNSLLLAPMPTASTAQILGNNECFEPYTSNIFT
                     RRVLAGEFIVVNKHLMRDLVKLGIWNEGLKNKIVAANGSVQNIPEIPQNLKDIYKTAW
                     EISQKAIIEMAADRGAFIDQSQSLNIFMENPNFGKLTSMHFYGWKKGLKTGMYYLRTK
                     AATDPIKFTLEKQANKEPLPLKERTEAERAADEALLKEQEMAAIACSLDNPEGCEMCG
                     S"
     gene            complement(3138..>3620)
                     /locus_tag="PK610_10385"
     CDS             complement(3138..>3620)
                     /locus_tag="PK610_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36386.1"
                     /translation="SKTIFLELASFLKKNRFTDHITQSAVWCVSDDHNVSGIYHSDRS
                     KVQPLRDVLCKLTGQKDVWYSTDTDHVMDDDGYIRCEPKLITGAIKCKADAPVGIRAE
                     VKGVDVEREYKIPGELKLPRAGEFEYDFNLRISGWQKGKYQVLIFAGDKKIHTQDFEL
                     "
BASE COUNT         1071 a          796 c          767 g          986 t
ORIGIN      
        1 gccgagtggg cactcaactg gatttctaag ggttcgtttg cagaaagatt agttgctttt
       61 gcagcagttg agggtatttt cttctccggt agtttttgct ccatattctg gcttaagaaa
      121 cgcggtttaa tgcccgggtt atccttttcc aatgaactga tctcgcgcga cgaaggactg
      181 cactgtgatt ttgcatgtct gctttattcg cagcacctgg taaacaaact cgacaaaaaa
      241 gtcgtagaac aaatcattac cgaggcagtt gccatcgaga aggagtttgt tacagacgcg
      301 cttcctgtga aactgatcgg aatgaatgca gacttaatga gtcagtacat tgaatttgtt
      361 gcagatcgtt tgttactgga gcttggaaat ccgaaagtct ataatgttac aaatccgttt
      421 gactttatgg acatgattaa tctccagggt aaaaccaact tctttgagaa acgtgtaggc
      481 gagtatcaaa aagcgggagt gatgtcgacc aaagaagaaa acaaattcag tctcgacgaa
      541 gaattctgat caaaacaact acaatacaat tacccaacat aacctaaaaa ccactacagc
      601 atgtttgtaa taaaaagaga cggccgcaag gaatcggtac aatttgacaa gatcactgca
      661 cggatcaaga aactgtgcta tgggcttaat cctattgtcg accccgtttc cgttgcgatt
      721 aaagtcgtgg agggtattta cgatggtgta actacatctg agctcgacaa tcttgcggca
      781 gaagtagcag ctaccaacac gatcaagcat ccggattatg ctttgcttgc atcgcgtatt
      841 gctgtttcga atctgcataa aaacacgaaa aagtccttct ctttaacgat gaaggatcta
      901 tatgattacg ttgatccccg caccggtgag cgtgcttcat tgcttgctga tgatgttgca
      961 gagatcatcg aaaaaaatgc tgagcttctt gactctgcta tcatttacga tcgtgatttc
     1021 cgtttcgact ttttcggatt taaaacatta gagcgttctt acctccttaa gctgcactcc
     1081 aaagtagcag agcgtccaca gcacatgttc atgcgtgttg ctattggaat tcataaggaa
     1141 gatcttaaga atgcgattga aacctatcat ttgatgagcg agggttggtt tacacatgct
     1201 actccaacac tcttcaatgc aggaactccg aagccacaaa tgtcttcttg tttccttctc
     1261 tctatgaaga aggattccat tacaggtatt tacgatacac ttacccaatg cgcaaagatc
     1321 agtcagagcg ctggtggtat cggattaagc attcacgata tccgcgcaac aggttcatac
     1381 attaagggaa caaacggaac atccaacggg attgtaccga tgctgcgtgt atttaacgat
     1441 actgcacgtt atgtagacca gggcggcgga aagcgcaaag gatcatttgc tatttacatt
     1501 gagccatggc atgccgatat tttcgatttt cttgatttga aaaagaacca cggtaaagaa
     1561 gagcaacgtg cgcgtgatct tttctatgca atgtggattc cggatctttt catgcagcgt
     1621 gttgaagaca acggagaatg gacactcatg tgcccgaacg aatgtcctgg cctaagcgat
     1681 acctacggta agaatttcga agatttatac acgaaatacg agcgcgaagg aaaaggccgc
     1741 aaaacaatta aagcgcagga tctttggttc aaaattcttg aatctcaaat tgaaactgga
     1801 acaccctaca tgctttacaa agatgcatgt aacgagaaat ccaaccagaa gaatttgggc
     1861 actataaaat catcaaatct ttgcacagag atcatggagt acacttcacc tgatgaagtt
     1921 gctgtatgta atcttgcatc gattgccttg ccgaagtttg ttaaagacgg agagttcgat
     1981 catcagcgtt tgtttgatat aacctaccat atcaccaaga acctcaacaa gattattgat
     2041 tacaacttct accccgttcc cgaagcacgt cgcagcaaca tgcgtcatcg tccaatcgga
     2101 ataggtgttc aaggtcttgc tgacgcattc attttgatgc gtcaaggttt tgacagtgat
     2161 gaagcgcgca aactcaacag cgaaattttc gaaacgatct attacgctgc acttacttct
     2221 tccaaagatc ttgcaaaaga atttggtccg tatgaaagct atgaaggatc acccatctcg
     2281 cagggtattt tccagcatga catgtggaat gtaacgccta gcccacgttg ggaatgggat
     2341 gtgttgcgcg aagagatcaa acagcacgga gtacgcaatt cactcttact tgcaccgatg
     2401 cctactgcat caactgcaca aattcttgga aacaacgaat gtttcgagcc ttacacttcc
     2461 aacatcttta cacgtcgtgt acttgccggt gagttcattg ttgtgaacaa gcacctcatg
     2521 cgtgatcttg tgaagcttgg aatctggaat gaaggattga agaacaagat tgttgctgcg
     2581 aatggttctg tacaaaacat tccggagatt cctcaaaatc tgaaagacat ctacaaaact
     2641 gcctgggaaa tttcgcagaa agcgattatt gaaatggcag ccgacagagg tgcattcatc
     2701 gatcagagtc agtccctgaa tattttcatg gagaatccaa actttggtaa actcacatcg
     2761 atgcacttct acggatggaa gaaaggctta aaaacaggaa tgtattacct gcgtaccaag
     2821 gctgcaactg atccgattaa gttcaccctt gaaaaacaag cgaacaagga gccacttcct
     2881 ctcaaggagc gcaccgaagc tgagcgtgct gcagatgaag cattactcaa agaacaagaa
     2941 atggctgcca ttgcctgctc tctcgataat cctgagggtt gcgaaatgtg cggaagctaa
     3001 atcataaaga tcgcctgcta cgatcttaca aatgctgttc tgtaacctcc taaatgcaga
     3061 acaacaacaa caacctacct cctaaaaagt cccgacgttt tgcgccggga ctttttaatt
     3121 taatgcagct tacattttta gagttcaaaa tcctgcgtgt ggattttttt gtctccggca
     3181 aaaatcaata cctggtactt ccctttttgc cagcccgaaa tgcgcagatt aaaatcatat
     3241 tcgaattctc cggcacgcgg caatttcagt tctcccggaa tcttatattc acgttcaaca
     3301 tctactccct ttacttctgc acgaattccc acaggagcat ccgctttgca tttaattgcg
     3361 ccggtgatta gtttaggttc acagcgaatg tatccatcat catccatcac atgatctgtg
     3421 tctgtggaat accacacgtc cttttgtccg gttaatttac aaagcacatc ccgcaatggt
     3481 tgaaccttcg aacgatctga atgataaatt ccggaaacat tgtggtcatc tgacacacac
     3541 catacagccg attgggtgat atgatcagta aaacgatttt tcttgaggaa ggatgccagc
     3601 tcaagaaaaa tcgttttact
//
LOCUS       DAOMYG010000329         3614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9244_length_3614_cov_8.620118, whole genome shotgun sequence.
ACCESSION   DAOMYG010000329 DAOMYG010000000
VERSION     DAOMYG010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9244_length_3614_cov_8.620118"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..2264
                     /locus_tag="PK610_10390"
     CDS             198..2264
                     /locus_tag="PK610_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRJ36387.1"
                     /translation="MEKADERTLLKAWELMCKAKALAELYERNKEVTAKYVHATSRGH
                     EAVQIALGIQLKAQDYLSAYYRDDAMLLSIGMTPYELMLQLLGKRDDPFSGGRHYYSH
                     PSLKRDHMPKIPMQSSATGMQAIPTTGVAMGLQYLEQTGMSPFSGDENPIAVCSLGDA
                     SITEGEVAEAFQMAVLKKLPILYLIQDNEWDISAHAREIRAQEAWEYAQGFKGLEVRR
                     INGSDFFESYAAIKEVIETIRKERRPFMVHAKVPLLNHHTSGVRKEWYREDLEEQMKR
                     DPYPLLRKQLIDSGIREGQLKELEIFAEMEVEKQYKQALEAEDPQPDDIYNAIFAPTP
                     ITEEKGQRSPAGKQPTVMVDSALFAIRELMSKHKECLLYGQDVGRRLGGVFREAATLA
                     QQFGDERVFNTPIQEAFIVGSTVGMSAVGLKPIVEVQFADYIWPGLNQLFTEVSRSNY
                     LSNGKWPVSMILRVPIGAYGSGGPYHSSSVESVVSNIKGIKVAYPSTGADLKGLMKSA
                     YYDPNPVVMLEHKGLYWSKLKGTEEARTIEPDEDYVIPFGKARMVQESDNKRAEKGET
                     LTIITYGMGVYWARNASKNLGITDQVEIVDLRTLVPYDEEMIFASVKKNGKCMVLTEE
                     PLTNSFAQALAGKIQEFCFHYLDAPVFARGAVDLPAIPLNSTLEAAMLPSAAKVEKWI
                     QEILAY"
     gene            complement(2319..>3614)
                     /locus_tag="PK610_10395"
     CDS             complement(2319..>3614)
                     /locus_tag="PK610_10395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRJ36388.1"
                     /translation="PSWKEFFSDQNLIRLIDTALVRNFDLMIAMQRIEAARAGVKRAK
                     GELLPDLNANVGAGTRKYADYTMDGVGNWDTRQSPNLVANPDRMLPDPIPDFYLGLSS
                     SWEIDIWGKLRNRKRAALARFLATEQGKNLIQTALVADVALAYYELMLLNRKVLILEE
                     NILLQQQALEMVRVQKITGDVDQLAVDFFEAQLLNSQGIELRTRYAIREVEARICILL
                     GQFPQPITVTPFNGVLPQLPILSSGVPSDILKRRPDILQSERELMASRADLRAARLAF
                     FPSLNISGALGWQAFTASLLLETPGSLAFQLLGGLTQPIFNRNALKAQLMQSKAEQKQ
                     AYLNYHKTIVGAFTEVYLLTMQSDFVERAYTLKSRETELNRNAVTISRELFVTGKASF
                     LDVITAQRNALNADLELIDVQYERLENRVRLYRSLGGGW"
BASE COUNT         1086 a          769 c          800 g          959 t
ORIGIN      
        1 gtcaatgccc tacttttttc tgaaaattgc ttaatgcatt tagctataca gtataaaatt
       61 ctgatgaatt tatgcttttc agtttaatat tttgattttt gaccaatcta tcaaacatta
      121 ttctttgttt tcattatatt gcagaataaa cattaagtca gggaagtatg agtgagttgc
      181 taaaaaaatc acctactatg gaaaaagcag acgagagaac actgcttaag gcgtgggaac
      241 tgatgtgcaa ggcaaaagct ttggcggaat tgtacgaacg taataaggaa gtgaccgcaa
      301 aatatgttca tgctacatcc agaggacatg aagctgttca gattgcgctc ggcattcaac
      361 tgaaagcgca ggattatctt tctgcctatt accgtgatga tgccatgtta ctttccattg
      421 gcatgacacc ctacgaattg atgttgcaat tgttgggtaa acgagatgat ccgttttcag
      481 gaggacgtca ttactattca catccttcac tcaagcgcga tcacatgccc aaaataccga
      541 tgcagtcctc tgccaccggt atgcaagcca ttcccacaac gggagtagcc atgggattac
      601 aataccttga acaaacagga atgagtccgt ttagcggaga tgaaaatcct attgcagttt
      661 gttcacttgg cgatgcttca attaccgaag gagaagttgc ggaagcattt cagatggcgg
      721 tattgaagaa acttcccatt ttatacctga tacaggataa tgaatgggat atctctgctc
      781 acgcacgtga aattcgcgca caggaagcct gggaatatgc acaaggattt aaaggattgg
      841 aagttcgtcg gataaacgga agtgattttt ttgaaagtta tgctgcaatc aaagaagtca
      901 ttgaaaccat tcgaaaagaa cgtcgtccat ttatggttca tgcaaaagtt ccattgctga
      961 atcaccacac atccggagtt cgcaaggaat ggtaccgcga agatctggaa gaacagatga
     1021 aacgcgatcc ttatccattg ctgcgcaaac agcttatcga ttccggaatt cgtgaaggtc
     1081 agttgaagga gttggaaatt ttcgctgaga tggaagtgga aaaacagtac aaacaagcac
     1141 ttgaagcaga agatccgcaa cccgacgata tttacaatgc catttttgca ccaacaccaa
     1201 ttaccgaaga aaaaggccaa cgatcaccgg caggaaaaca acctactgta atggttgatt
     1261 ccgctttgtt tgctattcgt gaattgatga gcaaacacaa agaatgtctt ttatacggac
     1321 aagatgttgg tcgcagactg ggcggagtat ttcgtgaagc agcaacactg gcacaacagt
     1381 ttggtgatga acgcgtattt aacacaccca ttcaggaagc atttattgtg gggtcgactg
     1441 ttggaatgag tgcagttgga ttaaaaccga ttgttgaagt acaatttgcc gactatatct
     1501 ggccgggtct caatcagctt ttcaccgaag tatcgcgatc gaactatctc tcgaacggaa
     1561 aatggcctgt gagcatgatt ttgcgtgtcc ccataggcgc atatggcagt ggtggaccat
     1621 atcactcttc aagtgtagaa tctgttgtga gcaatatcaa gggaataaaa gttgcctatc
     1681 cttccacggg agcagatctt aaaggattga tgaaatctgc ctattatgat ccgaacccgg
     1741 tagtgatgct ggagcacaaa ggattgtact ggagcaaact gaaaggaaca gaagaagcac
     1801 gaaccattga accggatgaa gattacgtca ttccattcgg aaaagcacga atggtgcagg
     1861 agtcggacaa caagcgcgca gaaaaaggtg aaacacttac catcatcacc tacggcatgg
     1921 gcgtttactg ggcgaggaat gcgagtaaga atttaggcat taccgatcag gttgaaattg
     1981 tggatttgcg tacgctggtt ccctatgatg aagaaatgat ttttgcatcc gtgaagaaaa
     2041 acggaaagtg tatggtgctg actgaagaac ctctaacaaa ttcttttgca caggcgcttg
     2101 caggaaaaat tcaggagttt tgttttcatt atctcgatgc tcctgtattt gcacgcggtg
     2161 cagtagatct tccggctatt ccacttaaca gtacactgga agcagcaatg ttacccagtg
     2221 cagcaaaagt ggaaaaatgg attcaggaaa ttcttgcgta ttaacagaag ttcatcaaca
     2281 gctgtattcc tgaattacgt tttctaacaa atacatcttt accaaccacc accaagtgaa
     2341 cggtaaagtc gcactctgtt ttcgagacgt tcgtattgta catcgatcaa ttcaagatct
     2401 gcattcagcg cattgcgttg tgcagtaatt acatcgagaa aacttgcttt tcctgtcaca
     2461 aacaattcgc gcgagatggt aactgcgttt ctgttcaatt cggtttcgcg cgatttcaac
     2521 gtgtaagccc gctcaacaaa atcggattgc atagtgagca ggtaaacttc cgtaaaggcc
     2581 cctacaattg ttttatggta attcaggtaa gcctgttttt gctcggcctt cgattgcatg
     2641 agttgtgctt ttaaagcatt gcggttaaaa atgggttgtg ttaatccacc aagcaattga
     2701 aatgccaacg atccgggagt ttcaagcagc aaacttgcag taaatgcctg ccaccccaat
     2761 gcgccactga tattgagcga aggaaaaaac gctaatcgcg cagcacgtag atcagcccgc
     2821 gatgccatta attcacgttc gctttgcaga atatccggac gacgttttaa aatatcggaa
     2881 ggaacaccag aagaaagtat tggtagctga ggaagtactc cattgaacgg tgttacggta
     2941 atcggttggg gaaattgtcc caacaatata catatccgcg cttcaacttc acggatggca
     3001 taacgtgtac gcaattcaat tccctgtgaa ttgagcaatt gtgcttcaaa gaaatcaact
     3061 gccaactgat caacatcacc tgtaattttt tgcacacgca ccatctcaag cgcctgttgc
     3121 tgtaaaagta tattctcctc gagaatcagc acttttctgt tcagcaacat caattcataa
     3181 taggctaatg cgacatcagc aaccagtgca gtctgaatta aatttttccc ttgctcagta
     3241 gccaaaaaac gagccagcgc cgcacgctta cgattgcgca gtttccccca tatatcaatt
     3301 tcccaggaag aagataatcc cagataaaaa tcaggaatcg gatccggcaa catacggtct
     3361 ggattggcaa ccaggtttgg agattgacgt gtatcccagt tacctactcc atccattgtg
     3421 tagtcggcat actttcgtgt tcctgcacca acattcgcat tcaaatccgg aagcaattcc
     3481 cctttcgcac gcttaacacc tgcacgggct gcttcaatac gctgcatggc gatcatcaaa
     3541 tcaaaattcc gaacaagcgc tgtgtcaatg agacgaatca ggttttgatc cgaaaaaaat
     3601 tctttccacg atgg
//
LOCUS       DAOMYG010000330         3606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9271_length_3606_cov_7.235708, whole genome shotgun sequence.
ACCESSION   DAOMYG010000330 DAOMYG010000000
VERSION     DAOMYG010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9271_length_3606_cov_7.235708"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PK610_10400"
     CDS             <1..226
                     /locus_tag="PK610_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36389.1"
                     /translation="IIRQGETRVEKAYNSFYILVEEPARLQIKSQNGFFELANTNANE
                     MQYEHHGKITIRHTGGAIARVRFIQVIPYK"
     gene            231..1103
                     /locus_tag="PK610_10405"
     CDS             231..1103
                     /locus_tag="PK610_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36390.1"
                     /translation="MEENENNIQPTIDTVEQPYDPASLENIKRVLDMSLKAGRPQSFE
                     IEVDGLRVVQRTPVLEMFDTYKTFIHPTTRKIIVTVYRGVRTPACTKHVFSRDNDKPL
                     NENLGNVQQQTTTATPPGFLSGTEVKQMMQDMLEKERMNNRIVSLEEKLDDREKKVKE
                     AEDYIMRLQTGIETLRQEVQRKGSQLLDKFMEIAKNPPDWIKLLILQNSKDGNKGLSG
                     TDDSEEEEVTIRKKQPALTEEEKEFVELMHRMEEKLEEDDYGLLIMINDKLIENEELI
                     EKVADLIDVKLPEE"
     gene            1200..1379
                     /locus_tag="PK610_10410"
     CDS             1200..1379
                     /locus_tag="PK610_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36391.1"
                     /translation="MKFEYDIVIEAPSEKEADQKAKALKTIAKKLSAKELAKLSDIIE
                     NDPIKLALAKKALGV"
     gene            1379..2191
                     /locus_tag="PK610_10415"
     CDS             1379..2191
                     /locus_tag="PK610_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36392.1"
                     /translation="MKRTKKHIKRKKSKQLGMVPVAIQAAAVAKDMTPEQKQAAIQAA
                     GGQPIAPAQDKLNLKEIVIYSTLGLVVAGGTIFLLSKAVRKQVANTQEKKSLDVDSSA
                     SVAKQIRMALDNDMWFGWGTDEEALRKVIRDIPSKDEFKKVAASYKKLYGNPLTADLQ
                     GDLSTAEYNEMMAIIAGKPEKKGDKVAYNHTAAAVRLYNAMSIWYGIFPGTDEDAIKA
                     VFMEIPTQADYKKIESAYYKKYGNILQDDLEGDLSQSYIDEYMGILHKKPKS"
     gene            2202..3176
                     /locus_tag="PK610_10420"
     CDS             2202..3176
                     /locus_tag="PK610_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36393.1"
                     /translation="MAEQTINTNQQPKKKNYKKAILIGLGVAATAVGGFFGWKWLKGK
                     DNVTHTENDADDAGETKSQTSSSSSSQSSGSGSSGSSWMNTSTPTTPVRKDEFPLKKG
                     SKGERVKAVQDALLYKYGKTILPKYGADGDFGSELEAGLTKAGYPKQIDESLYNIILK
                     SGSLNAKSTATNLKKAIDSKSYQGILDTVKTIRNLTDYKAVNEEYKKFTDWFLPPVQV
                     VTAAIAPFLSSDQRDKMRLEFIRIGLKFDGKVWTLSGLPAQEHPLLITTAPTELIDPK
                     NKVKVKVPAKMVVGYKLKEKNGLTLMRLLERNLKVIVKSSTVKIYEKH"
     gene            3163..3606
                     /locus_tag="PK610_10425"
     CDS             3163..3606
                     /locus_tag="PK610_10425"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRJ36394.1"
                     /translation="MRNIKYIVVHCTDTLSNAEVKAIQKYWKEHLKWKNPGYHYIIKA
                     NGEVEKLLDEKKPSNGVLGFNENCINICYIGGRTKDKKRVNTMTRAQENSLFDKIVAL
                     TEKYPGAEVKGHYEFPNQGGRICPNFNVKEWIKNYVPDLDQPDLD"
BASE COUNT         1268 a          788 c          767 g          783 t
ORIGIN      
        1 tatcattcgc caaggagaaa cccgcgtgga aaaagcctat aacagctttt acattttggt
       61 ggaagaaccc gcccgcttac agatcaaaag tcagaacgga ttttttgaac tggccaatac
      121 caacgccaat gaaatgcagt acgagcatca cggaaaaatc accatccggc ataccggggg
      181 agccattgcc agagtgcgct ttattcaagt catcccttat aaataaaaaa atggaagaaa
      241 acgaaaacaa catacaacct acgatcgaca ctgtggagca accctacgat ccggcttctt
      301 tggaaaacat caaacgcgtt ttggatatgt cgctcaaggc gggacgacct cagagttttg
      361 aaattgaagt ggacggtctt cgtgtagtgc agcgcacacc agtactggag atgttcgata
      421 cctacaaaac ttttattcat cccaccacgc gaaaaatcat tgttaccgta tatcgcggag
      481 ttagaactcc ggcttgcacc aagcacgttt tttcaagaga taatgataaa ccgttaaatg
      541 aaaatcttgg taacgtgcag caacaaacta ctacagcaac gcctccggga tttttaagcg
      601 gaacagaagt caaacaaatg atgcaggata tgctcgaaaa agagcgcatg aacaaccgta
      661 ttgtttcatt ggaagaaaaa ctcgatgacc gcgagaaaaa agtaaaggaa gcggaagatt
      721 acatcatgcg ccttcagaca ggaattgaaa cactccgtca ggaagtgcaa cgcaaaggat
      781 cgcaattact ggataagttc atggagattg ccaaaaaccc tccggactgg atcaaacttc
      841 tcatcctgca aaattccaaa gacggaaaca aaggattaag cggtacggat gattccgagg
      901 aagaagaagt gaccattcgc aaaaaacagc ctgcgctcac ggaagaagaa aaagaatttg
      961 tcgaactcat gcaccgcatg gaagaaaaac tcgaagagga tgattacgga ctgctcatta
     1021 tgatcaacga taaactcatc gaaaacgaag aactcattga aaaagtggcc gatctcattg
     1081 acgtaaagtt accggaagaa taatccctac taagaccaaa gaagaaaaaa acggagtcat
     1141 cacatgaccc cggcagggaa tccctgtgcc tgaaaaaaga aaaaaaacaa aaacagcata
     1201 tgaaatttga atatgacatt gtaattgaag ctccttcgga gaaggaagcc gatcaaaagg
     1261 cgaaagccct caaaaccatt gcgaaaaaac tctctgcaaa ggaactcgca aaactctcgg
     1321 acatcattga aaacgatccc atcaaacttg cccttgccaa aaaagcactt ggtgtgtaat
     1381 gaagcgaact aaaaaacata tcaaaagaaa aaagtccaaa caactcggaa tggttccggt
     1441 ggcaattcag gctgctgctg ttgcaaagga tatgaccccg gagcaaaagc aggcagccat
     1501 ccaagcagca ggtggacaac ccatcgctcc agcacaggat aaactcaacc tgaaagagat
     1561 tgtgatttat tccacccttg gactggtggt tgccggggga acgatttttc ttctttccaa
     1621 agccgtacgt aagcaggtgg ccaatacaca ggagaaaaaa tcgctcgatg tagattcctc
     1681 cgcttctgtt gccaaacaaa tccgcatggc tttggacaat gatatgtggt ttggttgggg
     1741 aaccgatgaa gaagcacttc gaaaagtcat ccgtgatatt ccctccaagg atgaattcaa
     1801 aaaagtagct gcttcctata aaaaacttta tggtaaccca ctcacggcag atctccaagg
     1861 ggatctctcc acagcagaat acaacgagat gatggccatc attgccggca agccggaaaa
     1921 gaaaggcgat aaagtagctt acaaccatac agctgctgcc gttcgcctct ataacgccat
     1981 gagcatttgg tacgggatat ttcccggaac ggatgaagat gccatcaaag ccgttttcat
     2041 ggaaatcccc acgcaggccg attacaaaaa gatcgaaagt gcatactaca aaaagtacgg
     2101 caacatcctg caagacgatc tcgaaggtga tttatcacaa tcctacatcg atgagtacat
     2161 gggcatcctt cacaaaaaac caaaatccta aatttttagc tatggccgaa caaacaatca
     2221 atactaacca acaacctaaa aaaaagaact acaaaaaggc gatccttatc ggtctgggtg
     2281 tagctgctac tgcggtaggt ggattttttg gctggaaatg gctcaaagga aaagataatg
     2341 ttacccatac agaaaatgat gcagacgatg caggcgaaac aaaatcgcaa accagcagca
     2401 gttcttcaag ccaatcttcc ggcagcggtt catccggttc ttcttggatg aatacctcca
     2461 caccgacaac tccggttcgc aaagatgaat tccctttgaa aaaaggaagt aaaggagaac
     2521 gagtaaaagc cgttcaggat gcattgctct ataaatacgg aaaaaccatt cttccgaaat
     2581 atggtgctga tggagatttt ggaagcgaat tagaagcggg tcttactaaa gcgggttatc
     2641 ccaaacaaat tgatgagagt ttatacaaca tcattttaaa aagtggatca ctcaacgcaa
     2701 aatcaactgc gaccaaccta aaaaaggcga ttgattcaaa aagctatcaa ggtattctcg
     2761 atacggttaa aacaatccgg aatctcaccg attataaagc cgtcaacgaa gaatataaaa
     2821 aattcaccga ttggtttttg cctccggttc aggtagtaac tgctgctatc gctccatttt
     2881 taagttctga tcaacgtgat aaaatgcgct tggagttcat tcgcatcggg ttgaaattcg
     2941 atgggaaggt ttggacgctt tcgggtttac cagcacagga acacccgcta ctgattacaa
     3001 ctgcacctac tgaactgatc gacccaaaga ataaggtgaa agtgaaagtc cctgcaaaaa
     3061 tggtagtggg ctacaaactc aaagagaaaa acggactaac gctcatgcgc ttgctggaga
     3121 gaaacctcaa agtcattgtc aaatcttcaa ctgtaaaaat ttatgagaaa cattaaatac
     3181 atcgtggttc actgcaccga tactctttcc aatgccgaag tgaaagcgat tcaaaagtat
     3241 tggaaagaac acctgaaatg gaaaaatccg ggataccatt acatcatcaa agcaaacggt
     3301 gaagtagaaa aactgctgga cgagaaaaaa ccatccaacg gagtattggg ctttaacgaa
     3361 aactgcatta atatctgtta catcggtgga cgaacaaagg ataaaaaacg cgtgaacacc
     3421 atgacccgcg ctcaggagaa ttcactattc gacaaaatcg tggcattgac cgaaaaatat
     3481 cccggtgctg aagtaaaagg tcattatgaa ttccccaacc aaggaggacg tatctgtcca
     3541 aacttcaatg taaaggagtg gatcaaaaat tatgtacctg atttggatca acctgatctc
     3601 gattaa
//
LOCUS       DAOMYG010000331         3601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9290_length_3601_cov_9.780598, whole genome shotgun sequence.
ACCESSION   DAOMYG010000331 DAOMYG010000000
VERSION     DAOMYG010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9290_length_3601_cov_9.780598"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PK610_10430"
     CDS             <1..287
                     /locus_tag="PK610_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36395.1"
                     /translation="VTSLQRLYWFTVEFGLVQEDGRQKIFGAGLASSFGESSEALHCD
                     KGDLRPFSLETIMATGFFTDRHQDFYFVLKEAQQLFDAFAEYRITLPCLT"
     gene            complement(265..1713)
                     /gene="pyk"
                     /locus_tag="PK610_10435"
     CDS             complement(265..1713)
                     /gene="pyk"
                     /locus_tag="PK610_10435"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRJ36396.1"
                     /translation="MKTLHPFILEKKTKIVATLGPASSSKEVLLEMMYNGANVFRINF
                     SHGEYEKHEAVIATIRELMTETGIHVAILADLQGPKLRCGEMENNGVLLETGQDYILS
                     TQKVVGNKERTYITYKEFPFDVKPGERVLMDDGKLVFEVTGTNGKDEVYTKVIQGGVL
                     GSKKGLNLPNTKVSLPSLTEKDKADLEFALEKDVDWIGLSFVREARDIIELKHIIASK
                     QKKAKVIAKIEKPEALEEIDDIIHESDGLMVARGDLGVEIPLQNVPLIQKMIIRKSLS
                     QGKPVIVATQMMESMITAMTPSRAEVNDVANAVFDGADAVMLSGETSVGKHPAKVIET
                     MSKIVMEAETFEGIYHKESIPNDPTNERFITDSICFNATRLGQRVGASAIITMTHSGY
                     TAFKISSQRPKAKIVVFTGNRKMLETLSLVWGVTGIYYDKMVSTDHTIADAKYMLKKL
                     KLVNEGELVINIASMPIEDNGKSNMLKLSRVE"
     gene            complement(1733..2152)
                     /locus_tag="PK610_10440"
     CDS             complement(1733..2152)
                     /locus_tag="PK610_10440"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HRJ36397.1"
                     /translation="MGKIKLEMEYDYDFDLVGISCHAPDYRLCWAINQKLGIGLHKRD
                     ALHVLSDRKKSTEDHFSIYEYFSEEDHIEYYLLPNKSNTAYLIPEQKAADYFFLLRNN
                     FAVELSELIAQLKSIEMVIAAFSIQAETLKSRENLLF"
     gene            complement(2241..2513)
                     /locus_tag="PK610_10445"
     CDS             complement(2241..2513)
                     /locus_tag="PK610_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36398.1"
                     /translation="MPKVRIQQHELECRTGENLRELLVRFDLVPEAGSQWGTCKGAIG
                     CGTCKLKVKGTIAAYEKEQGLVVKELNNEQIYSCKARVISDIEICG"
     gene            complement(2513..2776)
                     /locus_tag="PK610_10450"
     CDS             complement(2513..2776)
                     /locus_tag="PK610_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03643 family protein"
                     /protein_id="HRJ36399.1"
                     /translation="MQAQTDRIIEMAWEDRTPFEAIAFQFGISEKEVIALMRKHLLPS
                     SFRMWRKRVAGRTTKHAKLRAFTGGRFRCSRQRSISSNSISKR"
     gene            2865..3305
                     /locus_tag="PK610_10455"
     CDS             2865..3305
                     /locus_tag="PK610_10455"
                     /inference="COORDINATES: protein motif:HMM:NF021983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2452 domain-containing protein"
                     /protein_id="HRJ36400.1"
                     /translation="MQDSEEFQNPIDKDNITETPGSLPYAHHRGSAVIKTSKESVIKS
                     RSLSAMEDQTEMQLQQIRRQIELLAQQAQEIKERKELSLKIYQAKMNFAPLIGDAYYL
                     YETEEGGHILSMIGPNEWGRSRKFKVFLAKVRLLADHTWMKEEV"
     gene            complement(3302..>3601)
                     /locus_tag="PK610_10460"
     CDS             complement(3302..>3601)
                     /locus_tag="PK610_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36401.1"
                     /translation="MITKDKHVSEGIADMMLRGKYTTDLRLEEMVQLEWDEDSQSKKE
                     VVFFRVTCIMRSLLFATLVHEVTEVYGPEPRMFSFPVIQIEEDWANEVRQKTRKN"
BASE COUNT          956 a          849 c          739 g         1057 t
ORIGIN      
        1 aggttacttc attacagcga ctgtattggt ttacggtaga gttcggtttg gtgcaggaag
       61 acggaaggca aaaaattttc ggcgccgggc tggcttcttc cttcggagaa agttcggaag
      121 cactacattg cgataaagga gatcttcgtc cgttttcgct tgaaacaata atggcgaccg
      181 gattttttac cgatcgccat caggattttt attttgtgct gaaagaggct cagcaattgt
      241 ttgatgcatt tgcagaatac cggattactc taccctgctt aacttaagca tgttcgattt
      301 cccattgtct tcaattggca ttgatgcgat gttaatcacc aattcgcctt cgtttacaag
      361 cttgagtttt ttgagcatgt attttgcatc ggcaatggta tgatcggtgc ttaccatttt
      421 gtcgtaataa attcccgtaa caccccaaac cagcgaaagt gtttcgagca ttttccggtt
      481 tccggtaaac acaacaattt ttgctttggg tcgctgactc gaaattttga atgcagtata
      541 cccggaatgc gtcatggtaa taattgccga agcaccaacc cgttgtccga gacgtgttgc
      601 gttaaagcaa atactgtcgg taataaaacg ctcatttgtt ggatcattgg gaatagactc
      661 cttgtgataa attccctcaa atgtttccgc ctccattaca attttgctca tcgtttcaat
      721 cacttttgca ggatgttttc caacagaagt ttcaccgcta agcattactg catccgcacc
      781 atcaaaaaca gcgttggcta catcatttac ctcagcacgt gatggtgtca ttgccgtgat
      841 catactttcc atcatttgag tcgccacaat aaccggcttg ccctgggaca gtgatttacg
      901 aatgatcatt ttctgaatga gcggaacatt ctgcaaagga atctccactc ccaaatctcc
      961 gcgagcaacc attagtccat ccgactcatg aatgatatca tctatctcct caagagcttc
     1021 aggtttttca atttttgcga tcacttttgc ctttttctgc ttcgaggcaa tgatgtgttt
     1081 caactcaatg atatcgcgag cttcacgaac aaatgaaaga ccgatccagt ccacatcctt
     1141 ctcaagggca aactccagat ctgctttatc tttttcagta agagaaggca atgaaacttt
     1201 ggtattgggc aggttcaatc ctttcttgga tccgagtacg ccaccctgaa tgactttcgt
     1261 ataaacttcg tctttaccgt tagtgcctgt tacttcaaat accagtttac cgtcgtccat
     1321 caacaccctt tcgccgggtt ttacgtcgaa cggaaattcc ttataggtga tgtaagttct
     1381 ctccttgttc ccaacaactt tttgtgtgct gagaatataa tcctgtcccg tttccagcaa
     1441 cacaccgttg ttttccattt caccgcagcg cagtttgggt ccttgcaaat cggcgagaat
     1501 ggcaacatga ataccggtct cggtcatcaa ctcacgaatg gtggcaataa ccgcttcgtg
     1561 cttttcgtat tccccgtgtg aaaagttaat ccggaacaca tttgctccgt tgtacatcat
     1621 ctccagcaat acttcctttg aagaagatgc aggtccgagt gtagcaacaa ttttggtctt
     1681 tttttcgagg ataaatgggt gtaatgtttt caatgttaat cgtatttaag ttctaaaata
     1741 gcaggttctc gcgtgatttc aaagtttcgg cttgtatact gaaggcggca attaccattt
     1801 caattgattt tagctgtgca ataagctcag atagctccac cgcaaaattg ttgcgcagga
     1861 gaaaaaaata gtcggctgct ttctgttcgg gaatcagata agccgtattg cttttattgg
     1921 gaagaagata gtactcgata tgatcttcct cactgaaata ctcgtagatt gaaaaatgat
     1981 cttcggttga ttttttcctg tcgctcaata catgcagagc atctcgcttg tgcaatccga
     2041 ttcccagctt ttgattaatt gcccagcaca atctgtaatc cggagcgtga caactgattc
     2101 cgacaagatc gaaatcataa tcatattcca tttccagttt tatcttgccc attactttac
     2161 aaagtacgca agaaatcgga cagatacaat gtaaaggatt tattaagtgt gcgaacagcg
     2221 ctcgccaaca gaaggaagtc tcatccgcaa atctctatat cgcttatcac acgagcttta
     2281 caggaataga tttgttcgtt gttcaattcc ttcactacca atccctgctc tttttcatag
     2341 gcagctatgg ttcccttcac cttgagttta catgtaccgc aaccaatagc tcccttgcag
     2401 gtaccccatt gcgatccggc ttcaggaacc agatcaaaac gaaccaggag ttcgcggagg
     2461 ttttctccgg ttctgcattc cagttcatgc tgctgtattc gcaccttggg cattaacgtt
     2521 tcgaaattga gttggaactg atcgatcgct gcctgctgca acgaaatcgc cctccggtaa
     2581 aggcgcgcaa tttggcatgc ttggttgttc gacctgcaac acgctttctc cacatgcgaa
     2641 acgacgaggg caacaaatgc tttcgcataa gagcgatcac ttccttttcg gatataccga
     2701 actgaaaagc aatagcttca aacggtgttc gatcctccca ggccatttcg atgatacggt
     2761 cggtttgtgc ttgcataaag gcagtaggat ttacaacaag tcaaccggaa ttttgttctc
     2821 cctacaagca ttgccattaa ggcatttgac cttaaatttg ctttatgcag gactctgaag
     2881 agtttcaaaa cccgatcgat aaggataaca tcacggaaac accaggatcg cttccctatg
     2941 cgcatcacag agggagtgct gtaataaaaa cctccaagga aagtgtgatc aaaagccgct
     3001 cgctatcggc catggaagat cagacagaaa tgcaactgca gcaaatccgg cgacagatcg
     3061 aattactggc tcaacaggca caggaaatca aggagcgcaa agaactatcc ctgaaaattt
     3121 atcaggccaa aatgaacttt gctccactca ttggggatgc atattacctg tacgagacgg
     3181 aagaaggtgg acatatcctt tccatgattg gtccgaatga atggggccgc tcacgtaaat
     3241 tcaaggtctt ccttgcaaaa gtgcgcttac ttgccgacca cacctggatg aaagaagaag
     3301 tttagttttt acgcgtcttc tgacggactt cgttggccca gtcctcttcg atttgtatta
     3361 cgggaaaaga aaacatgcgg ggttcgggtc cgtaaacctc tgttacttca tgcaccaatg
     3421 tggcaaacaa caaagagcgc atgatgcagg taacacgaaa aaaaaccact tcctttttgc
     3481 tttgtgaatc ttcatcccat tccagctgca ccatttcctc caagcgaaga tccgtagtgt
     3541 atttccccct aagcatcata tccgcaattc cttcgctcac gtgcttgtct ttggtaatca
     3601 t
//
LOCUS       DAOMYG010000332         3598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9306_length_3598_cov_9.653401, whole genome shotgun sequence.
ACCESSION   DAOMYG010000332 DAOMYG010000000
VERSION     DAOMYG010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9306_length_3598_cov_9.653401"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3598
                     /locus_tag="PK610_10465"
     CDS             <1..>3598
                     /locus_tag="PK610_10465"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HRJ36402.1"
                     /translation="VSRNVLISDIGIIAKAGGDKFVNVFVSDLKNTQPMSGAKIDFYD
                     FQSQLIGSATADANGYAGIKLDTKPFVIIASKSNQRGYLRLRDGDALSVSKFDVGGES
                     VQRGVKGFIYAERGVWRPGDSIYLSFMIEDKEAVLPKNHPVSMEMVNPNGVVVQRMVK
                     TANVNGLYDFRTATQQEDPTGYYTVNINVGNRKYSKTLRIETVKPNRLKIYLDFGTDR
                     LSSSQEAAPVKLNSKWLHGAPAKNLAARVEVTVNSISTAFKGYTGFIFDDPTREFRAE
                     EQVVFDGALDENGDASFDPSLNIGDAAPGLLRAHFTSRVFEEGGDFSIDRSSVEYSPY
                     SNYVGIRVPEGDQFNGTLETDRDHIVDVVTVDEKGKAVSRRNIEVKVYSIRWRYWWDH
                     YEDDLSSYIANNSITPVLDTRINTTNGKGKFTLRVNRPSWGRFLVQVKDPESGHITGK
                     VVYIDWPYWARQNRKDKENATMLNFSTDKETYATGETVKISFPSPAGGRALISVESGI
                     RMVKYFWVETKSGETKFEFKATEEMAPNCYVNISLLQPYSVTENDLPLRLYGVLPISV
                     ENPDSHLKPSISMPDVIRPETQTTITVKEDQGKPMTYTLAMVDEGLLDLTSFKTPDPW
                     KFFNAREALGVKTWDLYDMVMGSFAMKMDKLLAIGGDGESTGKKGAKANRFKPMVYFS
                     GPHALKPGASATHKVDIPNYVGSVRVMVVSGQDLRYGHAEKTVAVRNPLMVLGTLPRV
                     LGPGEKVQLPVNVFAMEKHVKDVSVEVIPNEFLKPKNGTKKSIKFSKIGDEVVNFEFD
                     VAEKIGVAKVKIIARSGNEIARHEIELDVRTPNPKVTDVSEAMVEPGKSWNPNFSFNG
                     VEGTNKVTVEVSSIPTIDLGNRLKYLISYPHGCIEQTTSAVFPQLYLSTLMDLDNNFK
                     REISNNINAALRRIQLFQTAEGGFSYWPGESVASDWGSNYAGHFMLEAESKGYRIPSS
                     TKSAWIKYQQKLARNWRLGTRTNEYGYQQYDDLSQAYRLYTLALARQPEMGAMNRLRE
                     AKNLSNAARWRLAAAYQLAGQSDVCDQLLSTASTTIPKYTELSYTYGNDVRDKAMILE
                     VMSMTKQKAKSAALMKEVASALNENSWMSTQTTAYCLLGISKFIGSSPVDKTMKFSYS
                     VNGGELISKATQRPLFKAEHTPKQMNRSGKLSLKNTGDGTLYIKLSVEGNPVA"
BASE COUNT         1114 a          693 c          848 g          943 t
ORIGIN      
        1 ctgtaagcag aaatgtgttg atctccgata tcggaatcat tgcaaaggca ggaggagaca
       61 aatttgttaa tgtgtttgta agcgacctga agaatacgca accaatgagt ggagcgaaaa
      121 ttgattttta cgattttcaa tcgcaactga taggttctgc aactgccgat gccaatggtt
      181 atgccggaat taaactggac accaaaccat ttgttatcat cgcttcaaaa tcaaatcaac
      241 gtggttatct gcgtttgcgc gatggggatg cattatcggt aagtaagttt gatgtaggcg
      301 gggaaagtgt acagcgtgga gtaaaaggat ttatctatgc cgagcgcgga gtatggcgtc
      361 cgggtgattc catttacctc tcctttatga tcgaagataa agaagcggta ctaccaaaaa
      421 atcatccggt gagtatggaa atggttaatc caaacggagt ggttgtacaa cgcatggtga
      481 agaccgcaaa cgtgaacgga ttgtacgatt tcagaacagc tacacaacag gaagatccta
      541 ccggatatta tacagtaaat atcaatgtag gaaatcgtaa atacagcaaa actttacgta
      601 ttgaaacggt gaaaccgaat cgcttgaaaa tttatcttga tttcggaact gatcgactaa
      661 gttcatcgca ggaagcagct cctgtaaaat tgaattccaa atggctgcat ggtgcacctg
      721 ctaaaaatct ggccgcacgt gtggaggtta ctgtaaattc aatttcaacc gcttttaaag
      781 ggtataccgg atttatcttc gacgatccaa cacgtgaatt ccgtgcagaa gaacaagtgg
      841 tgttcgatgg agcacttgat gaaaacggag atgcaagttt cgatccttca cttaatatcg
      901 gagatgcggc acccggattg ttacgtgctc attttacatc acgtgtgttt gaagaaggtg
      961 gcgacttttc catcgataga agttcagtcg aatattctcc ctattccaat tatgtcggaa
     1021 ttcgcgttcc cgaaggagac cagtttaacg gaacactcga aaccgatcgc gatcatattg
     1081 ttgatgtagt aacagtggat gaaaaaggaa aagccgtttc gcgtcgtaac attgaagtta
     1141 aagtgtacag tattcgctgg aggtattggt gggatcatta cgaagatgat ttgtcgagtt
     1201 atatcgccaa caattccatt actccggtac tcgatacacg gatcaatact acaaacggaa
     1261 aaggaaaatt cacgcttcgg gtaaatcgcc cttcatgggg aagattcctc gtacaagtga
     1321 aagatccgga aagtggtcac attaccggaa aagtggtata tatcgattgg ccgtattggg
     1381 cacgacaaaa ccgtaaggac aaggaaaacg caacgatgtt gaatttttct accgataaag
     1441 aaacgtatgc aaccggggaa acagtgaaaa tttctttccc gagtcctgca ggcggacgcg
     1501 cactgataag tgtggaatct ggaattcgta tggtgaagta cttctgggtt gaaaccaaat
     1561 ccggcgaaac caaattcgaa ttcaaagcaa ccgaagaaat ggcgccgaat tgttatgtca
     1621 atatttcatt gctgcaaccg tattcggtaa ccgaaaatga tttgccattg cgtttgtatg
     1681 gagtgttgcc cattagtgtt gagaatcccg attctcacct taagccatcc atctccatgc
     1741 cggatgttat tcgtccggaa acacaaacaa caattacagt gaaggaagat cagggtaaac
     1801 cgatgacgta tacgcttgcc atggtggatg aaggtttact cgatctcact tcgtttaaaa
     1861 cgcctgatcc ctggaaattc tttaatgcac gtgaagcact cggtgtaaaa acctgggatt
     1921 tgtatgatat ggtaatggga tcgtttgcaa tgaaaatgga taaactgctt gcaatcggtg
     1981 gagatggtga gtcgacagga aaaaaaggag cgaaagcaaa ccgtttcaaa cccatggttt
     2041 atttttccgg tccgcatgca ttaaaacccg gcgcttcagc aacacataaa gtagatattc
     2101 cgaattatgt gggatctgtt cgtgtgatgg ttgtttcagg tcaggattta cgatatggtc
     2161 atgccgagaa aacagtagct gtacgcaatc cgctgatggt gcttggaaca ttgcctcgtg
     2221 tgcttggtcc gggtgaaaaa gttcaattgc ccgtaaatgt ctttgcaatg gaaaaacatg
     2281 ttaaggatgt tagtgtggaa gttattccta acgaattttt aaaacctaaa aacggaacaa
     2341 aaaaatcaat taagttcagc aagatcggtg atgaggtagt gaattttgaa ttcgatgtgg
     2401 ctgaaaaaat tggtgtggcg aaagttaaga tcattgcacg cagcggaaat gaaattgcgc
     2461 gtcacgaaat tgaattggat gtgcgaactc caaatcctaa agtgacagat gtatcagaag
     2521 caatggttga acccggtaaa tcatggaatc caaatttctc ctttaacgga gtagaaggaa
     2581 ccaataaggt tactgttgaa gtttcttcca ttccgaccat cgatctcgga aatcgtttga
     2641 agtacctgat ttcctacccg catggttgta ttgaacaaac aacatctgca gtatttccgc
     2701 aattgtattt gagtacatta atggatctcg ataacaattt taaacgcgag atcagcaaca
     2761 acatcaatgc tgcattgcga agaattcaat tgtttcaaac tgcagaaggt ggcttcagtt
     2821 actggcccgg agaatcggtt gcaagcgatt ggggttcgaa ttatgcagga catttcatgc
     2881 tcgaagcaga atccaaaggt tatcgaattc cctcctcgac aaaatcggcg tggatcaaat
     2941 accagcagaa actggcccgt aactggaggt tggggacacg taccaatgaa tatggatatc
     3001 agcaatacga tgatctttct caggcttatc gtctctatac gttagctctt gcacgccagc
     3061 cggaaatggg tgcgatgaac cgtttgcgtg aagcaaaaaa tctatcgaac gctgcacgat
     3121 ggagattggc cgctgcgtat caacttgcgg gacaaagtga tgtttgtgat cagttgttaa
     3181 gtactgccag cactacaatt cccaaataca cggagctatc ttatacctac ggtaacgatg
     3241 tgcgtgataa agcaatgata cttgaggtga tgtcgatgac caaacaaaaa gcaaaaagcg
     3301 cagcactcat gaaggaggtg gcttcagcgc ttaatgaaaa ttcctggatg agtacacaaa
     3361 caactgcata ttgcttattg ggaatcagca aattcattgg atcttctccg gtcgataaaa
     3421 caatgaagtt ttcctattct gtaaacggag gagaactcat ctcaaaagcc acacagcgtc
     3481 ctcttttcaa ggcagagcac acgccaaaac agatgaatcg ttccggaaaa ctgagtttga
     3541 aaaatacagg tgatggtaca ctgtatatca aattaagtgt agaaggaaat cctgttgc
//
LOCUS       DAOMYG010000333         3593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9316_length_3593_cov_8.627191, whole genome shotgun sequence.
ACCESSION   DAOMYG010000333 DAOMYG010000000
VERSION     DAOMYG010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9316_length_3593_cov_8.627191"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /locus_tag="PK610_10470"
     CDS             complement(<1..1470)
                     /locus_tag="PK610_10470"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HRJ36403.1"
                     /translation="MTRIRYTILVFLFIVTGNIAKAQIDTVFWFAAPWVTPDHHWRDP
                     IAFHFSTFNNPTTIRLQQPVLGYDTTFTVPANSLFSKTIHHMMNTIESKPANTVLDYG
                     FKITSSFPITVVYDVITRSPNFYNPETFSLKGQNGIGTEFVASFQTRWNNQTLGGDLN
                     GDGTVTQPYQQINVVATEDNTVLYITPRCAIVGHPANVTFSVTLPLKGQVYTIQNLVQ
                     NTNVVGNNLAGTIIVASKPVAVTLADDSVNPAGGGGCYDLMGDQTVPVDVIGKDYIVL
                     RGFLNAGSEESFFVTATENFTTVTINDGGVTTVVLNQGDTYRYQIAQPRTSVSADKNV
                     YVLHMTGYGCELGKAILPPLNCSGSDIVTFPRTNPQNFSINILCPAGAEGGFLLNGSA
                     ALVPAGSFSVVPGTGGQWLAAQLTFTTAQIPSGSSNILTNSIDNFGMGIINGGTSTGC
                     LYHYMSSFIRRVFVNAGNDQTLCTGSATVNLGGTVEGGAT"
     gene            complement(1480..2451)
                     /locus_tag="PK610_10475"
     CDS             complement(1480..2451)
                     /locus_tag="PK610_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRJ36404.1"
                     /translation="MKRILVILCSLILCGSQQLSAQQQPIFSSYLLNPFFYNPAVSGS
                     ENIHRLNVNYRDQWSGFEGAPKTFSASMYGSLKDRQKHGYGLMIVSDKIGLTARTGVY
                     ANYSYGIKLNKKTSLRLGISPGFLQYRVRLYDVRVFDGGDDLLTGNILSENALDINAG
                     FHLHNEKFFFGGSFSHVLGKAASFTTFNDQLRLHYNLMGGYEFKLNKKFDLQPIVVAR
                     YTQSAPVQADLTLRWLYMKNIWLSTSYRTSDAVSLGLGYIVKDRLWIGYTYDYTLTSI
                     RAYQGGSHEIGISYALTKKKPTLDDKDEELNNSIMDKYKKKKESEEQ"
     gene            complement(2463..>3593)
                     /locus_tag="PK610_10480"
     CDS             complement(2463..>3593)
                     /locus_tag="PK610_10480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ36405.1"
                     /translation="TEISNGNIILTLTSTNNGNCNAETSTMRIDFVAPPFANFNFSPV
                     CLNITNQFTDFSLPGFGSINQWTWDFGDGNGSTSQNPGNLYANDGTYPVSLIVSNTSG
                     CKDTIVQNVTVHPLPIADFTFTTACSGAIVTADFTDNSTISSGSLNYYFYDFGGLGSI
                     LAQNASQPFTGAGTFFITHIVSSANGCRDTIVKPLTINPRPSAGFYYYTPIGLNIGAP
                     VEFYDTSSFATSFLWSFGDQGATSTLENPTHVYFANGVYQVTQIVYDNLGCSDTARVF
                     ISINTVTTTISTLIPNALSPNGDGRNDTWKLPFLELLYPEATVEIFNRWGQKVFSSVG
                     YEVPWDGTMNGEPLPPGTYLFILDLKDQSQPDPITGSILLVR"
BASE COUNT         1061 a          721 c          766 g         1045 t
ORIGIN      
        1 tgttgctccg ccttctactg taccacctaa attaacagta gccgaaccgg tacataaagt
       61 ttggtcgtta cctgcattta caaaaacgcg acgaataaac gaagacatgt aatgatacaa
      121 acaacctgtt gaagttcctc cgttaataat acccattcca aaattatcga tagagttggt
      181 aaggatattg gaagatccag aggggatttg agcagtagta aatgttaact gtgcagcgag
      241 ccattgccct cctgtacctg gtaccacgct gaatgagcct gcagggacca atgctgcact
      301 tccgtttagt aaaaaacctc cctctgctcc tgccggacaa agaatattaa tagagaaatt
      361 ctgaggattt gtgcgaggga aagttacaat atcagatcct gaacagttaa gcggtggtaa
      421 aattgctttt cctaactcac aaccataacc ggtcatgtgt aacacataaa catttttgtc
      481 ggcagataca gatgtccttg gctgggcaat ctgataacgg taagtatcgc cctgatttaa
      541 gaccacagtt gttacaccgc catcgttaat tgttacagtt gtaaagttct cggtagctgt
      601 aacaaaaaat gattcttccg atcctgcatt caaaaatccg cgcagaacaa tataatcttt
      661 cccgataaca tcaacaggta ctgtttgatc tcccataagg tcataacatc caccgccacc
      721 agcaggattt acagagtcgt cggctagcgt tactgcaaca ggcttactcg ctacaatgat
      781 tgttccggca agattattac caacaacatt ggtgttctgt accaaattct gaattgtata
      841 aacctgacct ttcaagggta gtgttacaga aaaagtaaca ttcgccggat gtccaacaat
      901 agcgcatcgg ggagttatgt ataaaacggt attgtcttcg gttgctacaa catttatttg
      961 ctgatagggt tgtgtaactg ttccatctcc atttaaatca cctcccagtg tttggttgtt
     1021 ccaccgggtt tgaaaggatg cgacaaattc tgttcctatt ccgttttggc cttttaatga
     1081 gaaggtttcc gggttataaa aatttggaga acgggtgata acatcatata caactgtaat
     1141 cggaaaactt gaggtgatct taaacccata atccaaaaca gtatttgccg gcttgctttc
     1201 tatcgtattc atcatgtgat gaatcgtttt tgaaaacaat gaatttgccg ggacagtaaa
     1261 tgtggtatcg taacccaata caggttgttg caacctgatt gtagtcggat tattaaaagt
     1321 ggagaagtga aatgcaatgg ggtctctcca atggtgatcg ggtgttaccc atggtgctgc
     1381 gaaccaaaac acagtatcga tctgtgcttt tgcaatgtta cctgtaacta taaacaggaa
     1441 cacgagaatg gtatatctga tgcgagtcat atccgtggtt tattgctctt ctgattcttt
     1501 tttcttcttg tacttatcca tgatgctgtt gttcagctct tcatctttat cgtcaagcgt
     1561 aggtttcttt ttggtgagcg catagctgat tccaatttca tgcgaaccgc cctggtatgc
     1621 acgaattgag gtaagggtat agtcataggt atatccaatc cataagcgat ctttaacaat
     1681 atatccgagc cccagactta ctgcatcact tgtgcggtac gaagtggata accagatgtt
     1741 cttcatgtac aaccagcgca atgtaagatc tgcctgcaca ggtgcacttt gggtataacg
     1801 tgcaacaaca atgggttgga gatcgaattt tttattgagc ttaaattcat atcctcccat
     1861 aagattgtaa tggagtctta actgatcatt gaacgtggtg aagcttgctg ctttaccaag
     1921 aacatgactg aaggatcctc caaaaaagaa tttttcattg tgtaaatgaa atcctgcatt
     1981 aatatcaagg gcgttttctg ataaaatatt accagttaga agatcatcac ctccgtcaaa
     2041 aacgcgtaca tcataaagac gcactctgta ttggagaaac ccgggtgata tgcctaatcg
     2101 taaacttgtt tttttgttca gcttaattcc ataggaatag ttggcgtaaa ctccggtgcg
     2161 tgcggtgagt ccgattttat cagagacaat catcaatcca tatccgtgtt tctgacggtc
     2221 ttttaatgac ccgtacatgc tggcagaaaa tgtttttggt gcaccttcaa atcccgacca
     2281 ctgatcacgg tagtttacat ttagtcggtg aatattttca cttcctgaaa cagcagggtt
     2341 gtaaaagaaa gggttgagca gataactcga aaagatcggt tgctgctgag cggaaagttg
     2401 ttggcttccg cataagatca atgaacaaag tatgactaaa attcttttca tggtcggcgg
     2461 aattaacgaa cgagtaaaat tgatccggta ataggatccg gttgcgattg gtctttaaga
     2521 tcaagtatga acaggtaagt gcccggtggc aagggctccc cgttcattgt accatcccag
     2581 ggaacttcat aacccaccga agagaaaact ttttgtcccc agcggttgaa aatttctaca
     2641 gttgcttccg gataaagcaa ttcaagaaaa ggcaatttcc aggtatcatt tcttccatca
     2701 ccattgggcg ataatgcgtt cggaataagc gtagaaatag ttgtagtaac tgtattgatc
     2761 gaaatgaaaa cacgggcggt atcactacat cctaagttat catacacaat ttgtgttacc
     2821 tggtacactc cattggcaaa ataaacatgc gtcggatttt caagtgtaga ggtggctccc
     2881 tgatctccaa aactccacag gaaacttgtt gcgaaacttg aagtatcata aaattctacg
     2941 ggtgccccga tgtttaaacc aattggtgtg taataataga atcctgcact cggacgagga
     3001 ttgatggtaa gtggttttac gatggtgtcg cgacaaccat tagcagaaga aacgatatgt
     3061 gttatgaaaa aagtccctgc accggtaaag ggctgactcg cattttgcgc cagtattgaa
     3121 ccaagtccgc cgaaatcgta gaagtaataa ttcaatgatc cggaactgat cgttgagtta
     3181 tctgtgaaat cagcggttac tatcgcaccc gaacaagcag tggtaaaagt aaaatcagca
     3241 atgggtaaag gatgtacagt tacgttttgt acaatggtgt ctttacatcc tgaagtgttg
     3301 cttacaatta aggaaacagg gtaagtgcca tcgttagcat aaagattgcc tgggttttga
     3361 gaagtacttc cgtttccgtc tccaaaatcc caggtccatt gattaatgct tccaaaaccg
     3421 ggaagcgaaa aatctgtaaa ctgattggtg atattcaaac acacaggact gaaattgaaa
     3481 ttggcaaatg gaggtgcaac aaaatcaatt ctcatcgtgc ttgtctctgc attacaattt
     3541 ccgttgttgg tacttgtaag cgttagaata atgtttccat ttgaaatttc cgt
//
LOCUS       DAOMYG010000334         3590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9331_length_3590_cov_10.527298, whole genome shotgun sequence.
ACCESSION   DAOMYG010000334 DAOMYG010000000
VERSION     DAOMYG010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9331_length_3590_cov_10.527298"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3148
                     /gene="rpoC"
                     /locus_tag="PK610_10485"
     CDS             <1..3148
                     /gene="rpoC"
                     /locus_tag="PK610_10485"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015781745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRJ36406.1"
                     /translation="AAELFKPFIIRKMIERGIVKTVKSAKKIVDRKDPVVWDILENVL
                     KGHPVLLNRAPTLHRLGIQSFQPKLIEGKAIQLHPLVCTAFNADFDGDQMAVHVPLGN
                     AAILEAQLLMLASHNILNPSNGAPVTVPSQDMVLGLYYITKGRKSTEELTVKGEGMTF
                     YSPEEVTIAYNEGKMDLHAHIKVRTKDLDESGNLVDKLIETTCGRVLFNEVVPKEVGY
                     INELLTKKSLRDIIGRVLKAAGISKTAKFLDDIKDLGFYMAFKGGLSFNLEDVLVPEE
                     KEKMVQDAVKQVDEVMMNYNMGLITNNERYNQIIDIWTHTNSRLTHILMEKLKNHRQG
                     FNSIYMMFDSGARGSKEQIRQLSGMRGLMAKPQKSGATSQEIIENPILSNFKEGLSIL
                     DYFISTHGARKGLADTALKTADAGYLTRRLVDVAQDVIINADDCGTLRGLIATSLRKN
                     DEVVESLNDRILGRTSVHDVYHPQTGELLINAGEEITESIARKIEESPIEEVEIRSVL
                     TCELKRGVCAKCYGRNLATGRMVQMGEAVGVIAAQSIGEPGTQLTLRTFHVGGTASNI
                     ADESDIKAKYAGRIEFEELRTVAKQDSEGNPVDVVLGRSGELRVLDTNTGIVLQTANI
                     PYGSYVYVKNGDKLEKGALICRWDPYNAVILSEINGALKYGDIEEGITFREEVDEQTG
                     FREKVITESRDKKKVPSIQIVDVKSGEELKVYNLPVGAHISVDENKMVTAGEILAKIP
                     RMSGKTGDITGGLPRVTELFEARNPSNPAVVAEIDGVVSFGKIKRGNREIAVESRTGE
                     VRRYLVPLSRHILVQENDYVRAGQSLSDGAISPGDILAIEGPTKVQEYLVNEVQEVYR
                     LQGVKINDKHFEVIVRQMMRKVDIIEPGDTRFLEGQYASKIDFMEENDSLFGKKVVVE
                     SGDSANLKAGQIITARKLRDENSMLKRKDMRLVEARDAQPATSIPVLQGITRASLQTD
                     SFISAASFQETTKVLNEAAVNGKEDYLLGLKENVIVGHLIPCGTGTRSFQKALVYSQD
                     DYQNKVLKEKIREVELED"
     gene            3313..3465
                     /locus_tag="PK610_10490"
     CDS             3313..3465
                     /locus_tag="PK610_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36407.1"
                     /translation="MASNEKTSKTVASKAGKLLKSKKTSKAVKSVAASALTQAPDKKK
                     KTVKKK"
     gene            complement(3467..>3590)
                     /locus_tag="PK610_10495"
     CDS             complement(3467..>3590)
                     /locus_tag="PK610_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ36408.1"
                     /translation="IFKQLASEMKQTLLIVTHDQEFANKTDRIIEMEDGAIIRQ"
BASE COUNT         1026 a          737 c          855 g          972 t
ORIGIN      
        1 ggctgctgaa ctgttcaagc cgttcatcat tcgcaaaatg attgagcgtg gaatagtaaa
       61 aacagtgaag agcgctaaga aaattgttga ccgtaaggat ccggttgttt gggacattct
      121 tgagaatgta ctcaaaggac accctgtatt gcttaaccgt gctccaacac ttcaccgttt
      181 gggtattcaa tcgttccagc ctaagctgat cgaaggaaag gcaattcagc ttcacccctt
      241 ggtttgtact gcgttcaacg ccgacttcga tggtgaccag atggcggttc acgttccact
      301 tggaaacgct gctattcttg aagctcaatt gttgatgctt gcatcgcaca atatccttaa
      361 cccttctaac ggtgctcccg taactgttcc ttcgcaggac atggtgttgg gtctttacta
      421 catcaccaaa gggcgtaaga gcacagaaga acttaccgta aaaggagaag gaatgacctt
      481 ctattctcct gaggaagtaa ccattgccta caacgaaggc aagatggatc ttcacgctca
      541 cattaaggtg cgtacaaagg accttgatga gagcggaaac ctggtagaca aactgatcga
      601 aacaacttgc ggtcgtgttc tgtttaacga ggttgttcct aaggaagtag gttatatcaa
      661 tgaacttctt actaaaaaat ctcttcgtga tattatcggt cgcgtattga aagctgccgg
      721 aatttccaag actgcaaaat tcctcgacga cattaaggac ctcggttttt atatggcgtt
      781 taagggtggt ctttctttca atcttgagga cgtactcgtt cctgaggaaa aagagaaaat
      841 ggtacaagat gcggttaagc aagttgatga agtgatgatg aactataaca tgggtcttat
      901 caccaacaac gagcgttaca accagatcat tgatatctgg acgcatacca actctcgttt
      961 gactcatatc ctcatggaga aactgaagaa tcaccgtcag ggattcaaca gtatttacat
     1021 gatgttcgac tcaggagcgc gtggttctaa agagcagatt cgtcagctgt caggaatgcg
     1081 tggtctgatg gcgaagccgc aaaaatcagg tgctacttca caggagatca tcgagaatcc
     1141 gatcctttct aactttaaag aaggcctttc gattcttgac tacttcatct ccacccacgg
     1201 tgctcgtaaa ggtcttgcag ataccgctct taaaacagct gatgccggat acctcacacg
     1261 tcgtctggtt gacgttgcac aggatgtaat catcaatgct gacgattgtg gtacacttcg
     1321 tggtttgatt gctacatcac ttcgcaagaa tgatgaagtt gtggaatcac ttaacgatcg
     1381 tattcttggt cgtacttcag ttcatgatgt ttaccatccg caaacaggtg aattactcat
     1441 caatgcaggt gaagagatta cagaatctat cgctcgtaaa attgaagaat ctccaattga
     1501 agaagttgaa atccgttcgg tacttacctg cgaattgaag cgtggtgttt gtgcaaaatg
     1561 ctatggtcgt aacctcgcaa ccggtcgtat ggtgcagatg ggtgaagccg ttggtgttat
     1621 tgctgcacaa tcaattggtg aaccgggtac ccagcttaca ctccgtacat tccacgttgg
     1681 aggtacagca tcgaatattg cagatgagtc ggatatcaaa gcgaaatacg caggtcgaat
     1741 tgaattcgaa gaattgcgta ctgtagcaaa acaagattct gaaggcaatc ctgttgatgt
     1801 tgttctcgga cgttccggtg agctccgtgt gcttgatacc aataccggta ttgttttaca
     1861 aacagcgaat attccatacg gttcttacgt gtacgttaag aatggcgata agcttgaaaa
     1921 aggcgcattg atttgtcgtt gggatccata caacgcggta attctttctg agatcaacgg
     1981 tgcgttgaag tatggcgata tcgaagaagg tattacattc cgcgaagagg tcgatgaaca
     2041 aaccggcttc cgtgagaagg taattactga atctcgcgat aagaaaaaag ttccttcgat
     2101 tcaaatcgtt gatgtgaaat caggagaaga actcaaagta tataaccttc ctgttggcgc
     2161 acacatttct gtggatgaaa acaagatggt tactgccggt gaaattcttg cgaagattcc
     2221 tcgtatgtcc ggaaaaaccg gtgacatcac aggtggtctt ccacgtgtaa ctgaactttt
     2281 tgaagcacgt aatccatcca atcctgcggt tgttgccgag attgatggtg tggtaagctt
     2341 tggaaagatc aaacgtggta accgtgagat tgccgttgag agccgtacag gtgaagttcg
     2401 ccgctatttg gttcctcttt cacgtcacat tcttgttcag gagaacgact acgttcgcgc
     2461 aggacaatca ctttcagacg gagcaatcag ccctggtgat atccttgcga ttgaaggtcc
     2521 gacaaaagtt caggagtatc ttgtaaacga agttcaggaa gtatatcgtc tgcagggtgt
     2581 gaagatcaac gataagcact ttgaagtgat cgttcgtcag atgatgcgta aagttgatat
     2641 cattgagccg ggtgatactc gtttccttga gggacagtat gccagcaaga ttgatttcat
     2701 ggaagaaaac gattcactct ttggtaaaaa ggtggttgtt gaatccggag attctgcaaa
     2761 ccttaaagcc ggtcagatta tcactgcgcg taagttacgt gatgagaact cgatgctgaa
     2821 gcgtaaggat atgcgtcttg tagaagctcg cgatgctcaa cctgctacat ctattccggt
     2881 acttcagggt attacccgtg catcacttca aaccgacagc ttcatttctg ctgcatcttt
     2941 ccaggagact acaaaagtcc tcaacgaagc tgctgtaaac ggaaaagaag attacctgct
     3001 tggattgaaa gaaaacgtaa ttgttggaca ccttatccct tgcggaacag gtacccgttc
     3061 gttccagaaa gcactggtgt attctcagga tgactaccag aataaagtcc ttaaggagaa
     3121 aatccgcgag gttgaacttg aagactaatt attcaaattc agaatatcct aaaagccccg
     3181 gaactccggg gctttttttt gcgcatattt tctgcaatac agataggaat catacaaatg
     3241 aggatggtct gatgatggat gtcagcccgg aagcccggat ttggccttaa attgggtcta
     3301 aaatcgatta ttatggcaag caacgagaaa acatccaaaa cagtggctag caaggccgga
     3361 aagctgctta aaagcaagaa aacaagtaaa gctgtaaaaa gtgtagctgc ttctgcgctg
     3421 actcaggctc ctgacaagaa gaagaaaacg gtaaagaaga aataacctat tgccgaatga
     3481 tggcaccgtc ttccatttcg atgatgcggt ctgttttatt ggcaaattcc tgatcgtggg
     3541 ttacgatgag gagtgtttgt ttcatttctg aagccagttg cttaaagata
//
LOCUS       DAOMYG010000335         3584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9348_length_3584_cov_8.555398, whole genome shotgun sequence.
ACCESSION   DAOMYG010000335 DAOMYG010000000
VERSION     DAOMYG010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9348_length_3584_cov_8.555398"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PK610_10500"
     CDS             <1..705
                     /locus_tag="PK610_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HRJ36409.1"
                     /translation="SIVIIPTFNEKENIERILRKVFSLDKLFHVLIVDDGSPDGTAEI
                     VRSLMTEYPDRLFMEERASKSGLGTAYIHGFRWSLQHNYSFIFEMDADFSHNPDDLIR
                     LYNACAVDGFDMSVGSRYVKGGKVMNWPFTRWLMSYFASVYVRLILWINIKDTTAGFK
                     CYKRKALETIDLNKIRFVGYAFQIEMKYTALLHGFKIKEVPIVFIDRQLGQSKMSMRI
                     FKEAFWGVFKMRRKKI"
     gene            709..2043
                     /locus_tag="PK610_10505"
     CDS             709..2043
                     /locus_tag="PK610_10505"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRJ36410.1"
                     /translation="MATILIRNARIVNEGKITEGDVAIEGELIRKIGKNLEGNFDSII
                     DANGKFLLPGAIDDQVHFREPGLTHKGEIYTEAKAAVAGGITSYMEMPNTVPNTLTQE
                     LLEEKYKRAAEVSLANYSFFMGASNENLEEVLKTNPRNVCGIKIFMGSSTGNMLVDKR
                     EVLEAIFSKCKLLIATHCEDEGTVRRNNEEFKEKYGDQLNATHHPLIRSEEACWLSSS
                     MAVELAKKYNTRLHILHISTAKEVSLFRNDIPLKEKRITSEACIHHLWFSDADYLTKG
                     NFIKWNPSVKTATDRDGILKGVLDDRIDIIATDHAPHTFEEKSAPYSSAPSGGPLVQH
                     AIPAMLELVREGKITIETVVRKMSHAVADCFQIEKRGYIREGYFADLVLVDMNSEWTV
                     EKSNLLYKCGWSPFEGTRFHSQITHTIVSGHLVYENGEFNESKKGSRLLFNR"
     gene            2040..2399
                     /locus_tag="PK610_10510"
     CDS             2040..2399
                     /locus_tag="PK610_10510"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HRJ36411.1"
                     /translation="MRAIANSSLICILLFTIQACSGGSAQQEEPQVGILSEQHMKEIL
                     VELHLTEATVNEIYLPGDTIREGADEIYNDVFSSHGTNAKDFLQNIEYYSTKSNRLHK
                     IMEQVVDSLNTLDNPKQ"
     gene            complement(2400..>3584)
                     /locus_tag="PK610_10515"
     CDS             complement(2400..>3584)
                     /locus_tag="PK610_10515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ36412.1"
                     /translation="DVAHTMRGYMGDSLFFAGLKAIISNNQLQNLNSESFRDQLNALP
                     AIDVDDFFEDWVLHPGFPEFAIDSIQVVPAGSDFSVTVFVRQKLRGAPFYFDNVPLNV
                     FFRNANWAVETRKMIVSGQNDQVTFLLPFIPVQWSLNEDEKISEAVTADNWAVYSAGF
                     KSLPHSNFQVNFSQVPDSAFMRVEHHWIAADDFSTVDFLNVISPDRYWRISGIDWNNN
                     IVAEGRFAYNGSTSGSGYLDNQLMTNYGSVVFHEDSIRLFYRPHAGVNWVEYPYYTRQ
                     TQSSKTDKVGLIVADSLKLGDYAFGMRISALNIEEAVPQVELRLYPNPSDDMVWLEVD
                     GLMMDQYDVVVSDLFGRVVHSFPISGRKSGFSVADFASGIYFVKLHFGSNQIAVSKLI
                     VR"
BASE COUNT         1161 a          761 c          719 g          943 t
ORIGIN      
        1 agtatcgtca ttattccaac ctttaacgaa aaggaaaaca ttgaacgcat cctgcgtaaa
       61 gtgttctccc tggataagct ttttcatgta ttaatcgttg atgacggttc accagacgga
      121 actgctgaaa ttgtacgatc cttaatgaca gaataccctg atcgcttatt catggaagaa
      181 cgggcatcta aatcaggttt aggtacagca tatatccatg gatttcgctg gtcgctgcaa
      241 cataattatt cattcatttt tgaaatggat gctgatttct cgcataaccc cgatgatttg
      301 attcggttgt acaatgcctg cgcagtagac gggttcgata tgagtgtagg ttcacgctac
      361 gtaaaaggcg gaaaagtaat gaattggcca tttacccgat ggctgatgag ctattttgct
      421 tctgtttatg ttcgtctgat actttggata aatattaaag acaccactgc aggatttaag
      481 tgctacaaaa gaaaggctct cgaaacaatt gacttgaaca agattcgttt tgttggctac
      541 gcgtttcaga tagaaatgaa atacacagca ctattgcatg gcttcaaaat aaaggaagtt
      601 cccattgtat ttatcgatag acagttagga caatccaaaa tgagcatgag aatattcaaa
      661 gaggcttttt ggggtgtatt caaaatgaga agaaaaaaaa tttaagtcat ggctacgata
      721 ctgattcgca atgcaagaat cgtaaatgaa ggaaaaatca ctgaaggcga tgtagcaatt
      781 gaaggtgagc ttattcgtaa aatcggaaag aatttagaag ggaatttcga ttctataatt
      841 gacgctaacg ggaaattcct gttacccgga gcaatcgacg atcaggtaca ttttcgagag
      901 cccggcctaa cccataaggg agagatttac acagaagcaa aagcagcagt tgccggagga
      961 attacttcct atatggaaat gccaaatact gttcctaata cacttacaca agaattactt
     1021 gaagaaaaat acaagcgtgc tgcggaagtg tcacttgcga attactcctt ttttatgggg
     1081 gcatcgaatg aaaatctgga agaggtttta aaaacaaacc cgcgtaatgt atgtgggata
     1141 aaaattttca tgggctcgtc taccggcaat atgcttgtgg ataaacgtga agtgcttgag
     1201 gcgattttct ctaaatgcaa attacttatt gcaacccatt gcgaagacga aggcactgtt
     1261 cgacgtaaca atgaagaatt caaggaaaaa tatggtgatc agctcaacgc aacccaccac
     1321 ccgctaattc gcagcgaaga agcctgctgg ttatcttcat ccatggcagt tgaattggca
     1381 aaaaaataca acacacgtct tcatattctc cacatttcaa ctgcgaaaga agtctcatta
     1441 ttcagaaacg atattccgct aaaggaaaaa agaatcactt cagaagcttg tattcaccac
     1501 ctatggtttt cggatgcaga ttatcttaca aaaggaaatt tcatcaagtg gaatccatct
     1561 gtaaaaacag ctacagatcg tgacggaata ttgaaaggag ttcttgatga ccgcattgat
     1621 attattgcca ctgatcatgc tcctcatacg ttcgaagaaa aaagcgcccc ctattcatca
     1681 gcaccaagcg gtggtccgct tgtccagcac gctataccag caatgctcga gttggttcgc
     1741 gaagggaaga ttactattga aaccgttgtg cgaaaaatgt cgcatgcagt agccgattgt
     1801 ttccaaattg aaaaacgcgg ttacattcgc gaaggttatt tcgcagacct ggtgttggtg
     1861 gacatgaatt ctgagtggac ggtagaaaaa tcaaacctac tctacaaatg cgggtggtcg
     1921 ccttttgaag gaacgcgctt tcattcgcaa atcacacaca caatagttag tggtcacctg
     1981 gtttacgaaa acggggaatt caacgaaagc aaaaaaggta gtcgcctact tttcaatcga
     2041 tgagagccat tgctaattct tcgctaatct gcattctgct tttcaccatc caagcttgct
     2101 cgggagggag tgcgcagcag gaagaacctc aggtaggaat tctcagcgag caacacatga
     2161 aagaaatttt ggttgagctt catttaactg aagctacagt gaacgaaatt tatcttcctg
     2221 gcgatacaat ccgtgaagga gcagatgaaa tctacaacga tgttttctcc agccatggaa
     2281 ccaatgcaaa agatttcctg caaaacatcg aatactacag cactaaatca aatcgcttac
     2341 acaaaatcat ggagcaggtt gtcgactcgc taaacacgct cgacaatcca aagcaataat
     2401 cagcgcacaa ttaatttact cactgcgatt tgattcgatc caaaatgcag ttttacaaag
     2461 taaatcccag aagcgaaatc ggcaacactg aagccagatt tacgtcctga gataggaaat
     2521 gaatgaacaa ctcgtccaaa taaatcagag accacaacat cgtactgatc catcatcagg
     2581 ccatccactt ctagccacac catatcatca gacggattag gatatagccg aagttcaacc
     2641 tgcggaacag cctcctcaat gttcagcgca ctgatccgca ttccaaatgc ataatctcct
     2701 aacttcagcg agtccgcaac aatcaacccg accttatcgg ttttgctgga ttgcgtttgt
     2761 cgggtgtaat aagggtattc cacccaattc actcctgcat gcggacggta aaacaaacgg
     2821 atactatcct catgaaatac aacagagccg tagtttgtca ttaattgatt atccagatac
     2881 ccggaaccag aagttgatcc attataagca aatcgcccct ctgcaacaat gttattgttc
     2941 cagtcaatac cggaaatcct ccagtaccgg tcgggcgata ttacattcag aaaatcaaca
     3001 gtgctaaaat catctgcggc tatccaatgg tgttcaacgc gcataaatgc tgaatcagga
     3061 acctgtgaga aattaacttg aaaatttgaa tgcggcaatg atttaaatcc agctgaatac
     3121 accgcccaat tgtctgctgt aaccgcttca ctgatttttt catcctcgtt taatgaccat
     3181 tgtacaggaa taaatggcaa taaaaaagtt acctgatcat tctgcccgct tacaatcatt
     3241 ttacgtgttt ctacggccca gtttgcattc ctgaaaaata cgtttagcgg aacattatca
     3301 aaataaaaag gagcacctct taatttttgc cgaacgaaaa ctgttactga aaaatcactt
     3361 ccagccggaa ccacctgtat tgaatcaatc gcaaattccg gaaaaccggg atgcaatacc
     3421 caatcttcaa aaaaatcatc gacatcaata gcaggtaaag cgtttagctg atcacgaaat
     3481 gattcactgt taagattctg aagttggtta ttactaataa tagcttttaa cccggcaaaa
     3541 aacagggaat ctcccatgta tccacgcatt gtatgtgcaa catc
//
LOCUS       DAOMYG010000336         3575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9374_length_3575_cov_8.596591, whole genome shotgun sequence.
ACCESSION   DAOMYG010000336 DAOMYG010000000
VERSION     DAOMYG010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9374_length_3575_cov_8.596591"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2069)
                     /locus_tag="PK610_10520"
     CDS             complement(<1..2069)
                     /locus_tag="PK610_10520"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRJ36413.1"
                     /translation="MSLRALLTVFVLLPFFGYAQEFKFKHIGKEQGLSQSRVNAITED
                     KDGFLWMGTDDGLNRYNGREITVFKNNPGNDSTISDNWINVLFTDAGGQLWIGTLEGG
                     LNRYDKYFNRFEAFRADPRNEQALNSDNITAIAVNDENSFWVGTDNGLHLFDTKSKTF
                     KRFLDEMVNPNKPKTVLRNDNIRCLYRDKTGVLWIGTSRNGLYAYDSRSGEFEEHNDS
                     GLSEDLDNPYRMRIRTIFEDSNGQLWIGYDGGYLSRYDRKTTNYKYYISRENDSRSLS
                     NNRVTSIMEDHNGQLWIATADGINLYDPLLDNFRVVQAIDKNPFSMRDNFVRVLYEDK
                     AHSVWMGTDTYGATVYHRSIGKFNHINTDSYDAKGLLSNTIFCFAEESKERVWIGTIG
                     EGLAVYDRKQKTIAHFNREQNNTHNNILCLEKAVDKIWFGTWGGGLGCYDISKSDFSK
                     RPLDTENSYLSNNNILDIASDSKGHLWIATLRGLNYFDPENDSIRNWTKDEGLPHNVL
                     YCLHIDRKDRVWIGSNGGGLAMFDPANGVFKHWIKGGKNSLANNTVHCIVEDAKGNLW
                     LGTKNGLSRFEPASETFTNFTESDGLSNNFIVGILFDKGGNLWLSTYNGLTKFNPYAN
                     KDGIYESRKYFAIDGLQGDEFNQNAFFQASDGEFFFGGTQGFNHFYPSSIVDNPNIPP
                     VVITKFLV"
     gene            complement(2066..2488)
                     /locus_tag="PK610_10525"
     CDS             complement(2066..2488)
                     /locus_tag="PK610_10525"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010134490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRJ36414.1"
                     /translation="MNILIVNGPNLNLLGVREKSVYGDRSFDQHLDALRISFPQVVFS
                     YFQSNVEGELINCLHEFGFGRVDGIILNAGGYTHTSVALGDAVAAVATPVVEVHISNV
                     YAREEFRHHSLLAKHCKGVIAGFGLASYDLAVQSFLKA"
     gene            2516..3415
                     /gene="xerD"
                     /locus_tag="PK610_10530"
     CDS             2516..3415
                     /gene="xerD"
                     /locus_tag="PK610_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRJ36415.1"
                     /translation="MNWQIALRGFRSYLQLEKSLSLNSIEAYENDVRKLIDFANAENK
                     HPEDLRLEDLRRFITTLREIGLSATSQARILSGIRSFYNYLLLENEVKTDPTELLEMP
                     RVGRKLPDTLSIAEISSIIDAIDRSLTDGERNKAILEVLYSCGLRVSELTDLRISNIY
                     TRDGFIRIVGKGNKERLVPIGKPALDQIERYKQQLRDHQEIKKGNEDFLFLNQRGSKI
                     SRVYIFTLIKSLCLKAGIRKTISPHTFRHSFATHLVENGADLRAVQEMLGHESITTTE
                     IYTHLDRSFLKETILKYHPRAKN"
BASE COUNT         1003 a          836 c          761 g          975 t
ORIGIN      
        1 acgagaaatt tagttattac cactggtgga atgttcggat tatctacgat agaggatgga
       61 taaaaatgat tgaatccctg tgttcctcca aagaaaaact ctccgtcgct tgcctgaaaa
      121 aatgcatttt gattgaattc atcaccctgt aatccgtcga tggcgaagta ttttctgctc
      181 tcgtaaatcc catccttgtt ggcataagga ttgaatttgg taagaccatt gtaggtactc
      241 aaccataaat ttcctccttt gtcgaagaga attcctacaa taaaattatt gctcaatccg
      301 tcggattcgg tgaagttggt aaaggtttcg gaagccggtt caaaacgact caatccgttt
      361 ttcgtaccga gccataagtt tcctttagca tcttctacta tgcagtgcac cgtgttgttg
      421 gcaagactat ttttcccgcc tttgatccag tgtttgaaca ctccattcgc cggatcgaac
      481 attgccaatc caccaccatt gcttccgatc cacacgcggt ctttacgatc gatatgcaga
      541 caatacaata cattgtgcgg caatccctca tccttggtcc agttccggat cgaatcgttt
      601 tccggatcga aataattgag tccgcgcagt gtggcgatcc atagatgtcc cttgctatcg
      661 gatgcaatat ccaggatgtt gttattggag aggtaggaat tttcggtatc gagcggacgt
      721 ttggaaaaat cgcttttact gatatcgtag caacccaatc ccccacccca ggtaccaaac
      781 catattttat caactgcttt ttcgagacaa agaatattgt tgtgcgtatt gttttgctcg
      841 cggttaaaat gcgcaatggt tttttgtttg cgatcgtaaa ctgcaagtcc ctcaccaatg
      901 gttccaatcc aaacacgttc tttcgattct tcggcgaaac agaaaatggt atttgataac
      961 aatccttttg cgtcgtagct atctgtattg atgtgattga attttccgat actgcggtgg
     1021 tacacggttg ctccatatgt atcagtcccc atccacaccg aatgtgcttt gtcttcgtac
     1081 aacacgcgta caaaattatc gcgcatgctg aatggattct tatcgatcgc ctgcacaaca
     1141 cgaaaattat ccaatagcgg atcgtacaga ttgattccat ctgcagtggc gatccataat
     1201 tgaccgttat gatcttccat tatagaagta acgcggttgt tggataaact gcgggaatca
     1261 ttttcgcgac tgatgtagta tttgtaattc gttgtcttgc ggtcgtagcg actcaggtaa
     1321 ccaccatcgt aaccgatcca gagctgtccg ttgctatcct caaaaatcgt tctgatacgc
     1381 atccggtatg gattgtcgag atcttcggat aatccgctat cgttatgttc ttcaaattct
     1441 ccgctgcggg aatcgtaggc atacaatccg ttgcggctgg taccgatcca caacacaccc
     1501 gttttatcgc ggtacaaaca acgaatgtta tcgttgcgca ataccgtttt gggtttgttg
     1561 ggattcacca tttcatcaag aaagcgttta aaggttttgc ttttggtgtc gaagagatgc
     1621 aaaccattat cggtccctac ccaaaaactg ttttcatcat taacagcaat tgcagtaatg
     1681 ttatcgctgt tgagtgcctg ttcattgcgg ggatcagcac gaaatgcttc gaagcgattg
     1741 aaatatttat catagcggtt caatccccct tcgagcgtac cgatccagag ttgtccgcct
     1801 gcatcggtaa acaaaacatt gatccagtta tcgctaatcg tactgtcgtt acccggattg
     1861 tttttgaaaa cagtgatctc tcttccgttg tagcggttca gtccatcatc agtacccatc
     1921 cacaaaaatc catccttatc ctctgtgatg gcattcaccc tgctttgcga caaaccctgt
     1981 tccttgccga tgtgtttgaa tttaaattcc tgtgcgtatc cgaaaaacgg aagcaacacg
     2041 aatacagtca ggagcgcgcg caaactcatg ccttgaggaa ggattgaaca gcgagatcat
     2101 aggaagcgag tccgaaaccc gcaattactc ccttgcaatg tttggcgagc agagaatggt
     2161 gacgaaattc ctcacgcgca tatacgttgg aaatatgaac ttctactaca ggagtagcaa
     2221 cagcagccac agcatcacca agagccacac tggtatgtgt atagccgcca gcattgagga
     2281 taatgccatc caccctaccg aaaccgaatt cgtgcaggca attaatcaat tcaccctcca
     2341 cattgctttg gaaatacgaa aacacaacct gtggaaagga aatacgtaaa gcatccaggt
     2401 gctgatcgaa ggagcgatcg ccgtaaacag atttttccct tacaccaaga aggtttaaat
     2461 tcgggccgtt gacgatgagg atgttcatga aatcaaatgt aaccatttcc tgccaatgaa
     2521 ctggcaaatt gcactgcgcg gattccgctc ctaccttcag ctggagaaat cgctatcgct
     2581 caatagcatt gaggcttacg agaacgatgt gcgcaagctg atagacttcg ccaatgccga
     2641 aaacaaacat cccgaagacc tccgtctcga agatcttcgt cggttcatca ctactctccg
     2701 tgagatcgga ttaagcgcca cctcacaggc acgaatacta tcaggcatcc gttctttcta
     2761 caattatctg ctcctcgaaa acgaagtcaa aacagacccc accgaactcc tcgaaatgcc
     2821 acgcgtagga cgaaaactcc ccgatacgct gagcattgcg gaaatttcat ccattatcga
     2881 cgccatcgac aggagtctga ccgacggaga gcgtaacaaa gccattctgg aagtcctgta
     2941 ttcctgcggt ctgcgtgtaa gtgaactcac cgatctgcgc atttcgaaca tctacacacg
     3001 cgatggattt atccgcattg taggaaaagg gaacaaagag cgactggttc ccattggtaa
     3061 gccggcattg gatcagatag aacgctacaa gcagcaattg cgcgatcatc aggaaataaa
     3121 aaaaggcaat gaagattttc tctttctcaa tcaaagagga agtaaaatca gcagggtgta
     3181 tattttcaca ctcattaaat cgctatgtct gaaagcggga attcgtaaga cgattagtcc
     3241 gcatacattt cgacattctt tcgccacgca tttagtcgaa aacggagccg atttacgagc
     3301 ggttcaggag atgctggggc atgagagtat taccactacc gagatctata cgcatctgga
     3361 tcgcagtttt ctgaaagaaa cgatacttaa gtatcatcca agggcgaaaa attaaatgtg
     3421 cggatcacaa tttgcgtaag ctgagagaaa tgccttggcc gtaagtttca gggagtcgaa
     3481 gcttatgcaa atataacctg aaacgaattt cactataacg gatttatcgt tgtggaattc
     3541 caataaggag aaatcaaatg aaagaagatt ttaat
//
LOCUS       DAOMYG010000337         3573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9379_length_3573_cov_9.624787, whole genome shotgun sequence.
ACCESSION   DAOMYG010000337 DAOMYG010000000
VERSION     DAOMYG010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9379_length_3573_cov_9.624787"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..2479)
                     /locus_tag="PK610_10535"
     CDS             complement(56..2479)
                     /locus_tag="PK610_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRJ36416.1"
                     /translation="MKQLLFTALLAMQSLFNADAQKFDNVTWTTELKQNGCDAELIFT
                     ATIADGWYIYSQNQPNDNGPIPSSFTFKESNGYKRDGKVSEGKYKSKYMDGFGDTYNI
                     FEKKAVFKQKIKVLSKTDFEISGEIEFMQCDESKCLPPAYVPFSFKIKGADCGTTTVT
                     NENPENEGNEKETTQVSADTSGVISPVGWKIYSKKFNNSEYEIIIKPQGDEGWKVIND
                     QRSGTVKITFAFPAGIEANGELQVAETESFLSKKFGEFVVVKNDAFFTQKIKVTTTDE
                     AVLAKVPVAIDYIAVSDDKKAKLSELLTADVNLGEAENISTAESDQSYLGIFLIAFLS
                     GFAALLTPCVFPMIPMTVSFFLKSSKDRKKAISNAILYGVSIIVIYVVIGLLITSIFG
                     PTALNEMSTSLFFNLIFFAVLVLFAISFLGAFEIVLPSSWVNAADKNADKGGFLGIFF
                     MAFTLALVSFSCTGPIVGSLLVESVSKGIMGPIFGMFGFSLAIALPFTLFAIFPGFLN
                     NMPSSGGWLNTVKVSLGFIELALSLKFLSKADLVYQSHLLERELFLALFAGVLFMWGL
                     YLIGAFRLSHDSVSEKISTGRALMAVFVFSFMVYIIPGLWGAPVNLLAGIAPPLEYSE
                     SPYGVGNRAPEGGGGEDGGLPEHAAYGPHQLITFHDYHHGMDYAREKGLPAMIDFTGY
                     GCENCRKMEQKVWSDEKNLRLLRDSVVVISLHVDERTKLDPSDPASKQFRNVGQKWAD
                     MEVKRYGESSQPLYVLLDANEEMLNGKASFQTHGEIQGFNDWLIQGLKTYRQRKGVKI
                     VRPLMVLMD"
     gene            complement(2758..3492)
                     /locus_tag="PK610_10540"
     CDS             complement(2758..3492)
                     /locus_tag="PK610_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HRJ36417.1"
                     /translation="MEAALKQKAEKYYFLLGGVFIASLVACNLMFQKFFYWTPFSFLG
                     EGAEEGSAWHSITHYTFYISVGIIPYPVTFLITDIISEIYGRRRADMVVFAGLIASVF
                     VLLIIGIAAAVPATDWSPVSDSMFKSVFGSSAQAVAASMAAYLIAQFIDIRIFHFWKK
                     RTHGRHLWLRNNFSTIPSQFVDTFVVLFVLCWMQSIPWDRFGSLLINGFIFKVIIAVF
                     DTPFFYLLVWWFRKKFKLNPEEEIVF"
BASE COUNT         1109 a          802 c          719 g          943 t
ORIGIN      
        1 aacaataaaa aatccccgaa tcgattcggg gatttttgtt tttgatagtt tatttttaat
       61 ccatcagcac cattagtggg cgcacaatct taacgccttt gcgctggcga taggttttta
      121 atccctgaat cagccaatca ttaaaccctt gtatttctcc gtgcgtttga aaactcgctt
      181 ttccatttag catttcttca ttggcatcca acaacacata aagcggctga gaactttctc
      241 cgtagcgttt cacttccata tcggcccatt tctggccaac gtttctgaat tgtttgctag
      301 ccggatcgga aggatcgagt ttggtgcgtt catcgacatg cagagaaatc acaaccacag
      361 aatcgcggag taagcgcaga tttttttcgt cggaccaaac cttctgttcc atcttacggc
      421 agttttcgca accgtaaccg gtaaagtcaa tcattgcagg taatcccttt tcacgtgcat
      481 aatccatacc gtgatgataa tcgtgaaacg taatcaactg gtgaggtccg tatgcagcgt
      541 gctcaggcaa tcctccgtct tcaccacctc caccttcggg agcacgattt ccaactccgt
      601 aaggtgattc agaatactcc agaggaggag caattcctgc cagcagatta accggtgcgc
      661 cccacaaacc gggaatgatg tacaccatga aggagaaaac aaaaactgcc attaatgcac
      721 gaccggttga aattttttcg gaaacagaat catgcgataa acgaaatgcg ccgatgaggt
      781 acaatcccca catgaacaat accccggcaa agagagcgag gaacaattcg cgttcgagta
      841 gatgcgattg ataaacaaga tctgcctttg aaagaaattt caatgacaat gcaagttcaa
      901 taaatcctaa actaactttt accgtattga gccaacctcc cgaagaaggc atgttgttga
      961 ggaatcccgg aaaaattgca aaaagtgtaa acggaagtgc aatggctaat gagaatccga
     1021 acataccgaa aatcggtccc ataattcctt tggaaacgga ttctaccaag agcgaaccaa
     1081 caatgggtcc ggtacaggaa aacgaaacca acgcgagcgt aaaggccata aaaaagatgc
     1141 cgagaaatcc gcctttatca gcgtttttat cagcagcatt tacccaggaa gaaggaagca
     1201 ctatttcgaa tgcaccaagg aaggaaattg caaacaaaac taaaaccgca aagaagatca
     1261 ggttaaagaa aagagaagtc gacatctcat ttaatgccgt aggaccaaaa atactggtga
     1321 tgagcaatcc gataacgaca taaataacaa taatggatac accataaaga atggcattgc
     1381 tgattgcctt tttgcggtct ttacttgact ttaaaaagaa actcaccgtc ataggaatca
     1441 ttggaaacac acacggcgta agcagtgctg caaatccgct taaaaacgca ataagaaaaa
     1501 tacccaggta agattggtcg gattctgcag tagaaatatt ttctgcttca ccgagattaa
     1561 catctgcagt aagcaattca ctcagcttcg cttttttatc atctgaaaca gcaatgtaat
     1621 cgatggcaac cggcactttc gcaagaacgg cttcatcggt agtagtgacc tttatttttt
     1681 gcgtaaaaaa agcatcgttc tttaccacca caaactcacc gaatttttta gagagaaaac
     1741 tctctgtttc cgctacctga agttcgccat tcgcttcaat tcctgcagga aacgcaaacg
     1801 taatttttac tgttccggaa cgttgatcgt tgatgacttt ccatccttca tcaccttgtg
     1861 gtttaatgat gatctcgtat tctgaattat tgaatttttt ggaataaatt ttccaaccta
     1921 caggagaaat cacgccggaa gtatctgcac ttacctgagt ggtttccttt tcattgcctt
     1981 cgttttccgg attttcgttg gtaacggttg ttgttccgca atctgcgcct tttattttaa
     2041 aggaaaaagg aacatatgca ggaggcagac atttgctttc gtcgcactgc atgaactcga
     2101 tctctccgct gatttcgaaa tcggttttgg aaagtacttt tatcttttgt ttgaaaaccg
     2161 ctttcttttc aaagatgttg taggtatcgc caaagccgtc catgtatttc gacttgtact
     2221 ttccctcgct cactttacca tcgcgcttat aaccattgct ttccttaaag gtgaatgaag
     2281 aaggaatcgg accgttatca ttgggttgat tttgggaata aatgtaccaa ccgtccgcaa
     2341 tggtcgcagt gaaaataagt tcagcgtcgc aaccgttttg tttcagctcc gttgtccagg
     2401 tcacattatc gaacttctgt gcatccgcgt taaagagcga ctgcatggcc agtaatgctg
     2461 taaaaagcaa ttgtttcatg ggtacatttt tttccggttt caaacttagg gaattcccag
     2521 tggccgaagc gtgacagaaa ttactctcta tcaacagtgg tgcaacgggt aacaacagga
     2581 atttccacca aatcatgcag ctcaaaatca ggcccgaggg cttaaatcct acaaatctta
     2641 tcataacctt aatttgtagc tacaacacaa atataaagcg aaaaattgcc ccggagtccc
     2701 aaaaaatggc ccctatcctt ggatttctaa acttacgatt gagatagatt tgatagccta
     2761 aaacacgatt tcctcttccg gattcagctt aaacttttta cggaaccacc ataccaggag
     2821 atagaaaaag ggcgtgtcaa aaacggcaat gattacttta aagatgaaac cgttgatgag
     2881 cagcgaaccg aaacgatccc agggaatgga ttgcatccag cacagcacaa aaagcacaac
     2941 aaacgtatct acgaactgag aaggaatggt agaaaagtta tttctcagcc acaaatgtct
     3001 gccatgtgtg cgttttttcc agaaatgaaa aatgcggata tcgataaact gagcaatcag
     3061 gtaggctgcc attgatgctg ctaccgcctg tgcgctgctg ccaaacacac tcttaaacat
     3121 ggaatcactg acaggcgacc aatccgttgc cggaactgca gctgcaatgc caataatcaa
     3181 caaaacaaaa acagaagcga tcagcccggc aaacactacc atatcagccc gacgtcgtcc
     3241 atagatttca gaaataatat cagtgatgag aaaagtaacc gggtagggaa taattcccac
     3301 actgatgtaa aaagtgtaat gtgtaatgct atgccaggcg cttccttctt cagctccttc
     3361 acctagaaag gaaaagggtg tccagtaaaa gaacttctgg aacatgagat tgcatgcaac
     3421 cagtgaggca ataaatactc ctcctaacag aaaataatac ttctctgctt tttgtttgag
     3481 tgctgcttcc aaggcttaga taatcagatg ccggttaaca gctgaataaa ggagatgttc
     3541 atcctgcgta aatattcaca tcgtgaatac ggt
//
LOCUS       DAOMYG010000338         3563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9424_length_3563_cov_8.430730, whole genome shotgun sequence.
ACCESSION   DAOMYG010000338 DAOMYG010000000
VERSION     DAOMYG010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9424_length_3563_cov_8.430730"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PK610_10545"
     CDS             <1..458
                     /locus_tag="PK610_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRJ36418.1"
                     /translation="VRTKLRSLNTGKIYDVTFNSGVKIDTVRVERRNVQFLYKDESGY
                     HFMNTDDFEQIAVEEFQINSPQFLKESNEAEMMYDTDEDRVLSVELKPFVELEITYTE
                     PGIKGDTATNTLKPAKVETGAEVRVPLFINIGDKVRIDTRTGDYMERVK"
     gene            536..1625
                     /gene="prfB"
                     /locus_tag="PK610_10550"
     CDS             join(536..598,600..1625)
                     /gene="prfB"
                     /locus_tag="PK610_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_163284549.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRJ36419.1"
                     /translation="MITSEQYTKLVERLEALRGYLDIDRKLIDIQEEEIKTQDPNFWN
                     DNEKAEAVMKKIREMKFWTDGFSKAKTAIDDLTVLLEFHKEGGATEQEVEQQYAMSLE
                     YVEEMEFRNMLSSPEDKSSAILQITAGAGGTESCDWASMLMRMYLMYAQRMGYKTTEL
                     DLQEGDVAGVKQVTIQIDGDFAFGNLKGENGVHRLVRISPFDSNAKRHTSFVSVYVYP
                     LVDDNIDIVVNPSDLEWDTFRSGGAGGQNVNKVETAVRLHHKPSGIIIKNQESRSQHD
                     NRAKALQLLKSQLFEIEMRKRREASDKIEAGKKKIEWGSQIRSYVLHPYKLVKDLRTD
                     FETSDTVGVLDGKIDGFVKSYLMEFGGN"
     gene            1667..2017
                     /gene="arsC"
                     /locus_tag="PK610_10555"
     CDS             1667..2017
                     /gene="arsC"
                     /locus_tag="PK610_10555"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016479622.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HRJ36420.1"
                     /translation="MKKSITIYHNPRCGKSRCALELLRENGIEPKVVEYLKEVPDREA
                     MKLLLFRLHLRPHDIIRTGEEMYKKKLKGLNLNDDEWITIMLENPILIERPIVIAGNR
                     GVVARPPEKVLELI"
     gene            2065..3462
                     /gene="fumC"
                     /locus_tag="PK610_10560"
     CDS             2065..3462
                     /gene="fumC"
                     /locus_tag="PK610_10560"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HRJ36421.1"
                     /translation="MDYRIEKDTMGEVKVPADKYWGAQTERSRNNFKIGPEASMPKEV
                     IYAFAYLKKAAAHANHDLGVLTAEKRDLISKACDEILEKKLDDQFPLVIWQTGSGTQS
                     NMNVNEVIAYRAHVNSGGKLADEKKVLHPNDDVNKSQSSNDTYPTAMHIAAYKSAVEV
                     TIPGIRKLRDTLAKKAQEYNSIVKTGRTHFMDATPLTLGQEFSGYVQQLDNGIRSINN
                     ALEMVKELALGGTAVGTGLNTPKGYDVLVAKKIAEFTGHPFVTAPNKFEALAAHDAMV
                     ELSGAFKRVAVSLMKIANDIRMLSSGPRCGIGEIIIPDNEPGSSIMPGKVNPTQPEAM
                     TMVAAQVIGNDVAVSIGGSNGHFELNVFKPLIAANVLQSARLIGDVCVSFNDKCAEGI
                     EPNYAMLKQHLENSLMLVTALNTHIGYENAAKIAKKAHKENKTLREAAIELNLLTDAQ
                     FTEWVKPEDMVGSMK"
BASE COUNT         1118 a          639 c          841 g          965 t
ORIGIN      
        1 ttgtccgtac taaactcaga agtttaaata caggtaaaat ttatgatgtt acgtttaact
       61 ccggtgtgaa aatagacacc gtgcgtgttg agcgtcgcaa cgtacagttt ttgtataagg
      121 atgagtcggg ctatcatttt atgaatactg acgatttcga gcagattgca gttgaagagt
      181 ttcagattaa ttctccgcaa ttccttaaag aatcgaacga agcagaaatg atgtatgata
      241 ccgatgagga tcgtgtatta agtgtggagt taaaaccatt cgtggagttg gaaattacct
      301 atactgaacc aggaattaag ggagatactg caaccaacac actcaaacct gcgaaggttg
      361 aaaccggagc tgaagttcgt gttccgcttt tcatcaatat tggtgataag gttcgtatag
      421 acactcgtac gggtgactat atggagcgcg ttaagtgaca atttttattc ctgcatgact
      481 ctgtcgtaag gcagggtcat gttttttttt aatcatacac caaacaacta gcattatgat
      541 cacatccgag cagtacacga agctggtcga gcgcctggaa gcgctgaggg ggtatctttg
      601 acatcgatag aaaactgatc gacatccagg aagaagaaat caaaacgcag gacccgaatt
      661 tctggaacga taacgaaaag gctgaggctg ttatgaaaaa gatccgggag atgaaattct
      721 ggacagacgg tttttccaaa gcgaaaacgg ccattgatga ccttacggtt ttgcttgagt
      781 ttcataaaga aggaggagca acagaacagg aagttgaaca gcaatatgcg atgtcacttg
      841 agtatgtaga ggaaatggaa ttccgcaaca tgcttagctc tccagaagat aaatcgagtg
      901 ccattttgca gataactgcc ggtgctggag gaacagagag ttgcgattgg gcttcgatgc
      961 tgatgcgtat gtacctgatg tatgcgcaac gtatgggtta taaaactaca gaacttgatt
     1021 tgcaggaggg agatgttgcg ggagtaaaac aagtaaccat tcaaattgac ggagattttg
     1081 catttggtaa tttgaagggt gagaacggtg tacatcgttt ggttcgcatt tctccatttg
     1141 attccaatgc aaaacgacat acatcttttg tgtctgttta tgtttatccg cttgttgatg
     1201 ataatatcga tatcgttgtt aatccttctg acctggaatg ggatactttc cgttcgggtg
     1261 gtgccggtgg gcagaatgta aacaaagttg aaacagctgt tcgtcttcac cataaacctt
     1321 ccggtattat catcaaaaac caggagtcgc gttcgcaaca cgataaccgt gcaaaagcat
     1381 tacagttgtt gaaatcgcag cttttcgaaa ttgaaatgcg taaacgcaga gaagcatccg
     1441 acaaaatcga agcgggaaag aaaaagatcg aatggggatc tcagattcgt tcgtatgttt
     1501 tgcaccctta taaattagtg aaggatttac ggactgattt tgaaacttcg gataccgttg
     1561 gagtactgga tggaaaaatc gatggtttcg taaaatctta tctgatggaa ttcggaggaa
     1621 attaattttt gcatctcatt tcggatacag aaaagtaact gcgcttttga aaaaatcaat
     1681 tacaatatac cataatcccc gctgcggtaa aagccgctgt gcactggaat tgctcagaga
     1741 aaacggaatt gaaccaaaag tggtggagta tcttaaggaa gttcccgatc gggaagccat
     1801 gaaacttttg ctattccgat tacaccttcg tccacatgat attattcgta cgggtgaaga
     1861 gatgtacaaa aaaaagttga aaggattgaa tcttaatgac gatgaatgga ttaccattat
     1921 gttggaaaat ccaatactca ttgagcgtcc gattgtaatc gcagggaaca gaggggttgt
     1981 ggcgcgtcca ccggaaaaag tgcttgaatt gatttaaact gttttgtttt tcacttagat
     2041 ttactgaata aaatatcatg tattatggac tacagaattg aaaaagacac aatgggcgag
     2101 gttaaagttc ctgccgataa atattgggga gctcaaacag aacgttcgag aaacaatttt
     2161 aagatcggac cggaggcttc aatgcctaaa gaggttattt acgcattcgc atatttgaaa
     2221 aaagctgcag cacacgcgaa tcatgatttg ggagtgctca ctgctgaaaa acgtgatttg
     2281 atttctaaag catgtgatga aattctggag aagaaattgg acgatcagtt tccgctcgta
     2341 atctggcaaa ccggttcggg aacacaatcg aatatgaatg tgaatgaagt catcgcatac
     2401 cgtgcacatg tcaattcagg agggaaatta gccgatgaaa aaaaagtgct ccacccaaat
     2461 gatgatgtga ataaatcaca atcgtcaaat gatacttacc caactgcaat gcatattgca
     2521 gcatataaat ctgcagttga ggtgacaatt cccggaataa ggaagttgcg cgacacgctt
     2581 gcgaaaaagg cccaagaata caattctata gtcaaaacag gacgtactca ttttatggat
     2641 gcaactcctc ttacacttgg tcaggagttt tccggttacg ttcagcaact tgataatgga
     2701 attcgttcca ttaacaatgc gttggaaatg gtaaaagaac ttgcgcttgg tggtacagct
     2761 gttggaacag gattgaatac tccaaaggga tatgatgttc tggtggcaaa aaaaattgcc
     2821 gagtttacag ggcacccatt tgttaccgct cctaataaat tcgaagcatt agctgcacac
     2881 gacgcaatgg tagagttgtc gggcgcattt aaacgtgtgg ctgtttcact tatgaaaatt
     2941 gccaacgaca tacggatgtt aagttcaggt ccacgttgtg gcattgggga aatcattatt
     3001 cccgacaatg aacccggatc ttccatcatg cccggaaaag taaatcctac acagcctgag
     3061 gcaatgacca tggttgcggc acaggtaatc ggaaatgatg ttgctgtttc cattggagga
     3121 tcgaacggtc attttgaatt gaatgtgttt aagccactca tcgctgccaa tgttttgcag
     3181 agcgcgcgtc tcattggaga tgtgtgtgta agtttcaatg ataaatgtgc agagggaatt
     3241 gaaccgaatt atgcaatgct aaaacaacat cttgaaaatt cattgatgtt ggtaaccgca
     3301 cttaataccc atatcgggta tgaaaatgct gcaaaaattg ccaagaaagc ccacaaggaa
     3361 aacaaaactt tgcgtgaggc tgcaattgaa cttaatctgc ttaccgatgc ccaatttaca
     3421 gaatgggtga agccggaaga tatggtagga agtatgaagt aacaacgata aaccgattta
     3481 gaaaaggtgg tctgcggatc acctttttta gttttagcag agcagaggga cgagcatgat
     3541 tttacttatt ttcaaagtcc cat
//
LOCUS       DAOMYG010000339         3554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9463_length_3554_cov_8.832524, whole genome shotgun sequence.
ACCESSION   DAOMYG010000339 DAOMYG010000000
VERSION     DAOMYG010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9463_length_3554_cov_8.832524"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PK610_10565"
     CDS             <1..506
                     /locus_tag="PK610_10565"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HRJ36422.1"
                     /translation="GTMEDQPVVIACMDFGFIGGSMGAVVGEKIARAADMAMKKKCPL
                     IIISKSGGARMMEAGYSLMQMAKSSAKLTQLADAGLPYISFLTDPTTGGVTASYAMLG
                     DINVSEPRALIGFAGPRVVKETIGRDLPEGFQTAEFVLEKGFLDYIVERQHLKTELAK
                     SIRYMMS"
     gene            585..854
                     /gene="rpsO"
                     /locus_tag="PK610_10570"
     CDS             585..854
                     /gene="rpsO"
                     /locus_tag="PK610_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009414989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRJ36423.1"
                     /translation="MYLTSDVKTKIFKQHGGAEKNTGSTEGQIALFTHRINHLTGHLK
                     SNKKDKVTQKSLIDLVGKRKSLLDYLKAKNIAKYRELIAELNIRK"
     gene            960..3161
                     /locus_tag="PK610_10575"
     CDS             960..3161
                     /locus_tag="PK610_10575"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019976416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRJ36424.1"
                     /translation="MQLGFSTTIEIGNGKPITIESGKLAKQADGSVVVRQGNTMLLAT
                     VCSARDAKDGVDFMPLTVEYKEKYAARGIFPGGFFKRETRPSDHEILISRLIDRALRP
                     LFPDDYHADTQVAVFLISADEESLPDQLAGLAAGACLAVSDIPFNGPVSEVRVARIDG
                     ALQINPTKTDLERADLEMIVAGSTKEIIMVEGEMNEVSEDVMIEAIMFAHLAIKKQCE
                     ALNELASKFPKAAKKREYSHESNDEDLRKRVWDATYAKAYAVAKSGGGKDERGAGFKQ
                     IEEEFLASLSDEERAEKKSLVGRYYHDVEKEAMRRMIIDEGMRLDKRNTTQIRPIWCE
                     VDYLPGAHGSAIFTRGETQSLTTVTLGGTQDEQDIDGVIFKGSEKFLLHYNFPPFSTG
                     EARPMRATSRREIGHGNLALRGLKPVIPTGSENPYTVRVVSDILESNGSSSMATVCAG
                     TLALMDAGIQLRRPVSGIAMGLITDKKTGKYAILSDILGDEDHLGDMDFKVVGTANGI
                     TACQMDIKVDGLSEEILRNALAQAREGRLHILGELLKTLNTSRSEPKAHAPRIEYLEV
                     PQEVIGAIIGPGGKIIQEIQRTTTTTVSISEKDGKGIVEILAANKQNLEDALRMIKNI
                     AFPPQVEIGEVYKGKVRSIMPYGAFVEILPGTDGLLHVSELEWRRIDKVEEVLKEGDV
                     VEFKVLGKDPKNGKYKLSRKVLLPKPEGYVEPANTERGERRGPRKEEAKEA"
     gene            complement(3207..>3554)
                     /locus_tag="PK610_10580"
     CDS             complement(3207..>3554)
                     /locus_tag="PK610_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36425.1"
                     /translation="TIIARADGILHIAYTDIFLHLEDSKRIFRETRSFSPWEKAPLFL
                     SGTGFTNQDKASRDFNGSPEVLKHCSAIAFHSPSLAQKILANFFIKMMRPSVPTRFFT
                     TEEDAINWLKNYL"
BASE COUNT         1047 a          784 c          813 g          910 t
ORIGIN      
        1 atggaaccat ggaagaccag cctgtggtta ttgcatgtat ggattttggt ttcattggcg
       61 gttcgatggg cgccgttgta ggagaaaaaa ttgcacgtgc ggcagatatg gcgatgaaga
      121 agaaatgtcc gcttatcatc atctccaaat caggcggagc acgaatgatg gaagccgggt
      181 attcgcttat gcaaatggcc aaatcctcag cgaaactgac tcagcttgcc gatgctggtc
      241 tgccctatat ttccttcctt accgatccaa caacgggtgg agtaacagct tcatacgcca
      301 tgctgggcga tatcaacgtc tccgagcccc gcgcgctcat tggttttgct ggtccgcgtg
      361 tggttaaaga aaccattgga agggaccttc ccgaaggttt ccaaaccgct gagtttgtgc
      421 ttgaaaaagg attcctcgat tatattgttg aacgccaaca tcttaaaacg gaactggcga
      481 aatccatccg atatatgatg tcctaatgat tttttttgct ctcttgttgt gtaggagaga
      541 aaatcattta tctttgcagc ctgttagaaa ttaagaaaga tcacatgtac ctgacatccg
      601 acgtcaaaac caaaatcttc aagcagcacg gtggtgctga gaaaaacacc ggtagcacag
      661 aagggcaaat cgctcttttc actcaccgta tcaaccattt aaccggtcac ctgaagagca
      721 ataaaaaaga taaagtaacc cagaaatccc ttatcgacct cgtaggtaag cgcaagagtc
      781 ttcttgacta cctaaaggct aagaacatcg cgaaatacag ggaactgatc gcagaactta
      841 acatccggaa ataaccggta tgaacgaata aaaaggggca attgtgaata acgattgcct
      901 cttttatttt tattccagcg taaataaaca gaagtatata cacctaaaga agaaaaaaga
      961 tgcaattagg tttttcaaca acaattgaaa ttggaaatgg caagcccatt accattgaat
     1021 ccggcaaact tgccaaacag gccgatgggt cagtagtcgt tcgtcagggc aataccatgc
     1081 tcctggcaac agtatgttct gcacgtgatg caaaagatgg cgtagatttt atgccgctta
     1141 ctgtagaata caaagagaaa tatgctgcac gaggaatttt ccccggcgga tttttcaaac
     1201 gcgaaacacg tccgagcgat catgagatcc tcatcagccg ccttatcgac cgtgcccttc
     1261 gtccactctt ccccgacgat taccatgccg atacacaggt tgcggtattc cttatttctg
     1321 ccgatgaaga aagtctgccc gatcaacttg ccggtttagc cgcaggtgca tgtttggcag
     1381 tgtctgatat tcctttcaac ggtccggttt ctgaagtacg tgttgcgcgt atcgacggag
     1441 ctttgcagat caatccaacc aaaaccgatc ttgaacgtgc tgatcttgaa atgatcgttg
     1501 caggttctac caaagagatc atcatggtgg aaggggaaat gaatgaagtt tctgaagatg
     1561 tgatgatcga agccattatg tttgcgcacc ttgccatcaa aaagcagtgt gaagcactca
     1621 atgaactcgc atcaaaattc cccaaagcag cgaaaaaacg cgaatactca cacgaatcca
     1681 acgatgaaga tctgcgtaag cgtgtttggg atgctaccta cgcaaaagct tatgccgttg
     1741 ccaaatccgg cggtggaaaa gacgaacgtg gtgccggttt taaacagatc gaagaagaat
     1801 tcctcgccag cctttctgat gaagaacgtg ctgagaaaaa atctttggtt ggacgttact
     1861 accacgatgt agaaaaagaa gcaatgcgtc ggatgatcat cgacgaaggt atgcgtctgg
     1921 ataagcgtaa caccacacag atccgtccga tctggtgcga ggtagattac cttcccggtg
     1981 cacacggttc tgctattttc acacgcggtg aaacacaatc gctcacaact gttacccttg
     2041 gtggaacaca ggatgagcag gatattgatg gtgtgatttt caaaggatcg gaaaagttcc
     2101 ttttgcatta caacttccct cctttctcca ccggtgaagc acggcctatg cgtgcaacca
     2161 gccgtcgcga gattggtcac ggtaacctcg cattgcgcgg actcaaacca gttattccta
     2221 caggatctga gaatccttac accgtgcgtg tagtttccga tatcctcgaa tcgaacggat
     2281 cttcttctat ggctactgtt tgtgcaggta cacttgcact tatggatgca ggtattcagc
     2341 ttcgtcgccc tgtttcgggt atcgcgatgg gattgatcac cgataagaag accggtaaat
     2401 acgctatcct ttccgatatc ctcggtgatg aagatcattt gggtgatatg gactttaaag
     2461 ttgtaggaac tgcaaacggt attactgctt gtcagatgga cattaaagta gacggacttt
     2521 ctgaagagat tctgcgcaat gcacttgcac aggcccgcga aggaagattg catatcctcg
     2581 gcgaattact caagacgctc aacacttcac gttccgaacc gaaggctcat gctccgcgta
     2641 ttgagtacct cgaagttcca caggaagtta ttggtgcgat tattggtccc ggcggtaaaa
     2701 tcattcagga aattcagcgt accactacaa ctactgtttc tatctctgaa aaagacggaa
     2761 aaggaattgt tgaaattctt gcagccaaca agcaaaatct tgaagatgca ttacgcatga
     2821 tcaagaacat cgctttccct ccacaagtgg aaattggtga agtatataag ggtaaagtac
     2881 gttcaattat gccttatggt gcattcgtgg aaattcttcc cggtaccgat ggtttgttgc
     2941 acgtttctga actcgagtgg cgcagaattg ataaggttga agaagtattg aaagaaggag
     3001 atgttgtaga atttaaagtt ttaggaaaag accctaaaaa cggtaaatac aaactctcac
     3061 gcaaagttct tctgccaaag cctgaaggct atgtagaacc tgccaataca gaacgcggcg
     3121 aacgtcgtgg tccacgcaaa gaagaagcaa aagaagctta atcttcataa agaaaaaaag
     3181 ccccggatga ccggggcttt tttcatttac aaataattct tcagccagtt tattgcatct
     3241 tcctctgtag taaaaaagcg ggtgggtaca gaaggacgca tcatctttat gaaaaagttg
     3301 gccaatattt tttgcgccag cgaaggcgaa tgaaatgcaa tagcagaaca atgctttaat
     3361 acttccggac tcccgttaaa atcacgcgag gctttatcct gattggtaaa cccagttcca
     3421 gaaagaaaca aaggcgcttt ttcccatgga gaaaaggatc tcgtttctct gaaaatcctt
     3481 tttgaatctt ctagatgcaa gaaaatatcc gtatatgcta tatgtaaaat accatctgca
     3541 cgagcgataa tggt
//
LOCUS       DAOMYG010000340         3532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9539_length_3532_cov_9.971240, whole genome shotgun sequence.
ACCESSION   DAOMYG010000340 DAOMYG010000000
VERSION     DAOMYG010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9539_length_3532_cov_9.971240"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="PK610_10585"
     CDS             <1..1333
                     /locus_tag="PK610_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36426.1"
                     /translation="FDIPFEEFILDAEVGGVYTVRSGGGSRIILPPSVMVDSYGDLLQ
                     GDIKFRYREFHDAADIYCAGIPMDYDAAGMIKRFETAGMFELRAYDIRGNEVFVDSGK
                     TMSVSFAGTITGSEYHFFYLDEKGTRNWHYIGDEGGVFNPEKEKIRRRKQASALKIPL
                     GPDYFSFNYMAALDVMLNDNVQQINKNRSSADFRTKISEYGLGWSNIYNYQSVQFEGR
                     TMLASLLVWKNIGGKKLPDWAGKSDSKLVPLEGNVYELQLIEKGGTRQFSTRIEPFMS
                     LKALFAFSAAEWKSNYEKTLQRVKEDEKRLTMLADVYREMEINRFGIYNYDRFLNDKN
                     AVVVKADFRFDRDAGILQNQEVCYVSGTDRALIKYPFEKWSQFILLPDPGAQLFALLP
                     GNFIAVFSPKEYNKVDQKKLKLETEPAVVFELHTVRQFNGAEDLRMILDGK"
     gene            1330..1728
                     /locus_tag="PK610_10590"
     CDS             1330..1728
                     /locus_tag="PK610_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36427.1"
                     /translation="MIAYIYPLLILLTGAITLALKYNKRINEEDRFKWIIPAFFYFCF
                     FIASGLILFFVWPASTSTSGLGAVAISVTSVLYSIWLWVVFTAVYAVVAVILLMSYGK
                     KNRHLDGVSSGVTITLALLGVLVLGLVIML"
     gene            1841..2275
                     /locus_tag="PK610_10595"
     CDS             1841..2275
                     /locus_tag="PK610_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36428.1"
                     /translation="MKLFGSRLAPIFFFWIIFLNASSLFANAELDLSPENDVVFLQVN
                     DDENAEVINESQEEKVFPVPNIHSPLSEPDLSFPGFSGEPEEENISQKEKDGKDGVYL
                     HDQLLLHWIESSLSHLPFINYSRRNCPVPLFVLHHSWKSFLI"
     gene            2340..>3532
                     /locus_tag="PK610_10600"
     CDS             2340..>3532
                     /locus_tag="PK610_10600"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsB"
                     /protein_id="HRJ36429.1"
                     /translation="MDNHSLVFDEQHSLEHLKHYLPAQAALKDFVHHNTLHAFQHEKF
                     HTALRQASNVFGYTTYLSIDEYRERFNSGKIREEILNRTIDEYITKGLALGFNQLKEA
                     VLYKNLDVPISGKIGSLRSQWDKHCSIALDKIIPQILFRLLAAFLDQGIAFWKFPYKN
                     SSFLEAVRNLERNSAVSLFHSAKAKELLLDESITLTDLLFMVVGRESLFEHYLFDQQF
                     AHPGWSGFVAMLETQPETLLDKRKIELRELIILELILEIDALENKFRKGWKPLASVLV
                     EEPDSLFSSYTPVEADLIRSVWQDAFEWSFYDDVLSGLEEAEDVTANSPASMQALLCI
                     DDREYSFRRHIQLVDPLSSTFGTPGFFGVEFYFRPENGKFNTKACPAPVNPSFLIKET
                     NRKAA"
BASE COUNT          987 a          673 c          773 g         1099 t
ORIGIN      
        1 attcgatatt ccttttgaag aatttattct tgacgctgaa gtcgggggtg tgtacaccgt
       61 tcgttcaggt gggggttcaa gaattatttt gccaccttct gtgatggtgg atagttatgg
      121 agacctgctt caaggcgata taaaatttcg ttaccgtgaa tttcatgatg cagccgatat
      181 atattgcgca ggtattccaa tggattatga tgcggcagga atgatcaagc gatttgaaac
      241 tgcaggaatg tttgagttgc gagcatacga catccggggg aatgaagtgt ttgtcgatag
      301 cggaaaaacc atgtccgttt catttgcagg aacaattacc ggaagcgaat atcatttttt
      361 ctacctcgat gaaaaaggaa cccgaaactg gcattacatc ggagatgaag gaggcgtttt
      421 taatccggaa aaagaaaaaa tccgtcgtcg caaacaagca tcagcactga aaatcccgct
      481 tggaccggat tacttttcat tcaactacat ggcagcactt gatgtgatgt tgaatgataa
      541 tgttcagcag atcaataaaa acaggagcag cgccgatttc cgtacaaaaa tttcggagta
      601 cggattgggt tggtcgaata tttacaacta ccaaagtgtg caattcgaag gcagaacgat
      661 gcttgcttcg ctgctggttt ggaaaaatat cggcggaaaa aaactacccg actgggccgg
      721 gaagtcggat agcaaacttg ttcctcttga gggaaacgtt tatgaattgc aattaattga
      781 aaagggagga acgcgtcagt tttctacccg tatagagccg tttatgtctt taaaggcact
      841 ttttgcattc tctgctgccg aatggaaaag caattacgaa aaaacactgc agcgtgtaaa
      901 agaagatgaa aagcgtctga ccatgcttgc agatgtctac cgtgaaatgg agatcaatcg
      961 ttttggcatt tataactacg atcggttcct gaacgataaa aatgcggtcg ttgtaaaagc
     1021 agattttcgt ttcgaccgtg atgccggcat attgcagaac caggaagtat gttacgtaag
     1081 cggaacggat cgtgcattga taaaatatcc ctttgagaaa tggtcgcagt tcattcttct
     1141 tcctgacccc ggagcacaac tgttcgcatt gttacccgga aattttattg cggttttttc
     1201 accgaaagaa tacaataagg tggatcagaa aaaacttaag ctggaaacag aacctgcagt
     1261 ggtgtttgag ttgcatacgg taaggcaatt caatggcgcg gaagatctcc gcatgatcct
     1321 cgatggtaaa tgatcgctta tatttatcca ttacttattt tgcttaccgg agcaattaca
     1381 cttgctttga aatacaacaa acggatcaat gaagaagacc gtttcaagtg gatcatacct
     1441 gcattcttct atttctgctt ttttattgca agcggactaa ttctgttttt tgtttggccg
     1501 gcatcaactt ctacatcagg acttggtgct gttgcaatat ctgtgacgtc agtattgtat
     1561 tcaatatggt tgtgggtagt ctttactgca gtttatgccg ttgtagccgt aattttgctg
     1621 atgagttatg gtaaaaaaaa caggcatctt gatggtgtaa gttccggagt taccattacg
     1681 ctggcactat tgggagtgct ggttttggga cttgtcatca tgctttagtt aatgttaaac
     1741 aactcattta ttgtgagtta atccctgttt cgttacgcaa ctgttaattt ttttgaggag
     1801 agctgattcc tctgtttatt tgtacttgca ttcaacacct atgaagcttt ttggcagtcg
     1861 tttggcacct atctttttct tctggataat tttcctgaat gcatcttcat tatttgccaa
     1921 tgctgaatta gatctctcgc ctgagaacga tgttgttttt ctgcaagtca atgatgatga
     1981 aaatgcagag gtgatcaatg aatcgcagga agaaaaagta tttcctgtcc ccaatattca
     2041 ttctccgtta agtgagccgg atctgtcctt tcccggtttt tccggtgaac ctgaagaaga
     2101 aaatatttcg caaaaggaaa aagacggaaa agatggtgtt tatctacacg accagctatt
     2161 gttacactgg atagaatctt ctctttccca tttaccattt ataaattatt ccaggcgcaa
     2221 ttgcccggtt cccttgttcg tccttcacca ttcctggaaa agttttttga tctgaataaa
     2281 cgcattgcga gtttggcttc tatgccaatt gtcaattagt attatttaga atcaacacca
     2341 tggataatca ttccctggta tttgacgaac aacattcctt agagcatcta aagcattatt
     2401 tacccgctca ggcagcactc aaggatttcg ttcatcacaa taccttacat gcttttcagc
     2461 atgaaaaatt tcatacagca ttaaggcaag cctcgaatgt atttgggtat actacctatt
     2521 tatcaattga tgaatacaga gagcgattta attccggtaa gatcagagag gagattctta
     2581 atcgaaccat cgacgagtat atcactaaag gtcttgcctt gggttttaat caacttaagg
     2641 aagctgttct ttataaaaac cttgacgtcc caatttcagg taaaatcgga tcgcttcgtt
     2701 cgcaatggga taagcattgt tcaattgctt tggataagat cattcctcaa attttattca
     2761 gactattagc ggcattcctc gaccagggta ttgcattttg gaagtttcct tacaaaaaca
     2821 gttcgttttt agaggctgtt cgcaaccttg aaaggaacag tgcagtaagc ctttttcatt
     2881 cggcaaaagc aaaagaactg ttgcttgatg aaagcattac tcttactgat ttgttgttta
     2941 tggtggtcgg acgcgaatca ttgtttgaac actatctttt tgatcagcaa tttgcacacc
     3001 cgggttggtc gggttttgtt gcaatgctcg aaacacagcc ggagacactt cttgacaaac
     3061 gcaaaatcga gttaagagaa cttattatcc tggagttgat tctggagatc gatgcgcttg
     3121 aaaataaatt tagaaagggt tggaaaccgc ttgcatcagt actcgttgaa gaacctgatt
     3181 cacttttttc aagttatact ccggttgagg cagacctcat tcgtagtgtc tggcaagatg
     3241 ctttcgaatg gagtttttac gacgatgttt tgtccggact ggaagaagct gaagatgtaa
     3301 cagcgaattc accggcttcc atgcaggcct tgttgtgtat tgatgatcgc gaatactctt
     3361 tcagacgaca tattcaattg gtcgatccgc tttcgtctac gtttggtact ccgggatttt
     3421 ttggtgttga attttatttt cgaccggaaa acggaaagtt caatacaaag gcttgtcccg
     3481 ctccggtaaa tccttctttt ctgattaaag aaaccaacag aaaagcggca tt
//
LOCUS       DAOMYG010000341         3525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9564_length_3525_cov_6.973199, whole genome shotgun sequence.
ACCESSION   DAOMYG010000341 DAOMYG010000000
VERSION     DAOMYG010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9564_length_3525_cov_6.973199"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..773
                     /locus_tag="PK610_10605"
     CDS             156..773
                     /locus_tag="PK610_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRJ36430.1"
                     /translation="MRKILSVLFFVCCVAPLLAQDNISPEWDKETLARANSAAECTYM
                     SADEKQIIFYCNLARMNGKLFATTYAKQYYKDKKVKSTSYSKSLITELNKLDPLPPFQ
                     PAEDLYNAAFAHAVTSGKKGTTGHQQFESRFKKHAPGYNPYGENCSYGNHRPLDFVMS
                     LLIDEGIKGLGHRKNIFSKQFSHVGVAIHTHKKYKYNAVMDFGKK"
     gene            complement(812..1207)
                     /locus_tag="PK610_10610"
     CDS             complement(812..1207)
                     /locus_tag="PK610_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ36431.1"
                     /translation="MESNKIKIWLADDSPVYRTLITQELRMGLGAEVKTFPDGDSLIA
                     AYAKNSPPDLILLDFHFDFDDHENNGSRTLRKLREMGVQTPAVLVTGVSEPEFVAERK
                     QEDFIDVIDKFHDDMIGHLKEIISSIGKK"
     gene            1269..1772
                     /locus_tag="PK610_10615"
     CDS             1269..1772
                     /locus_tag="PK610_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36432.1"
                     /translation="MNKWIVPSILSLSLLVSCAKPKYRSEEGGFAVSFKGIPQENTEN
                     VATAHGDIAVTMFLDEVSPDMVYLVGYSDYPEAVLAATSPEAHLMKTAENVAESVKGT
                     LSDVKKNSVNGFPSLDFRSVGNPFSVAYRLIIRSTRMYQVGIMKQKEAIKDSEIQGFI
                     ETFEILE"
     gene            1853..2011
                     /gene="rpmH"
                     /locus_tag="PK610_10620"
     CDS             1853..2011
                     /gene="rpmH"
                     /locus_tag="PK610_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRJ36433.1"
                     /translation="MKRTFQPSLRKRRNKHGFRERMASQNGRRVLAARRKRGRHKLTV
                     SSEKRHKR"
     gene            complement(2265..>3525)
                     /locus_tag="PK610_10625"
     CDS             complement(2265..>3525)
                     /locus_tag="PK610_10625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRJ36434.1"
                     /translation="EVIRTMNEAIFDRKFQYSCHIKLLQTEVENRLFYYERGTGTMIS
                     PNEILIRKEDEEKVVWGDNIILATGSRPRKLPNVTVDEKVIMTSDGIHHMTDFPKSLV
                     IVGAGVIGCEFATIFANFGKTKVYIIDRADRILPFEDEDISDLVSHNLEEKGVTIHHN
                     AQLERLVNVDGEVEYELSYSDGRREVIRVEKALLAIGRVPNIEELGLNEAGVHMSKRG
                     VHIGDVDTRTSVPHIYAVGDVSGRIQLVNVGELEARHAVEKISMGDDMVNPLSYDNIS
                     TIMFLHPEVAAVGMNEKQCREKNIPVKVVKVDYSVIARAIAMRKTQGFFKIIVTNDNE
                     NKIIGMRAIGEHASSAIQACALLIKMGKGIDELAEMIHPHPSIIEGVQECVRMLLNKS
                     VFKSSVFKDKLKCYSCVNGECTPLQRL"
BASE COUNT         1008 a          769 c          724 g         1024 t
ORIGIN      
        1 tggagtagtg gccattttgc aaaactattg aaaattgtga taggaagttt gaattgaata
       61 caaatcgtaa aatacgtata gaaataaggg aactacctta tattataata ttctaaattt
      121 tgtaaccttg ggcctttatt ttctgtagaa tgctcatgcg caaaatcctt tctgttttat
      181 ttttcgtttg ttgtgtagcg cccttattgg cacaggataa tatctcgccc gaatgggata
      241 aggaaacgct cgcccgggca aattctgcag cggaatgtac ctacatgagc gcagatgaaa
      301 agcagatcat cttttactgc aatcttgcac gaatgaatgg aaaacttttt gccacaactt
      361 atgccaaaca atattataag gataaaaaag ttaagagtac ttcctattcc aaatccctga
      421 ttacggagtt gaataaactc gacccattac caccatttca acccgcagaa gatttgtaca
      481 atgctgcatt tgcccacgca gttaccagtg gaaaaaaagg gacaaccggc catcagcaat
      541 ttgaatcgcg atttaaaaaa catgctccgg gttataatcc ttatggcgaa aactgctcgt
      601 acggaaacca tcgtccgctc gattttgtca tgagtctcct catcgatgaa ggcataaaag
      661 gtctaggtca ccggaaaaat atattcagca aacaattttc acatgttggt gttgccattc
      721 atacgcataa aaaatacaag tacaatgcag tgatggattt tggtaaaaag taaaatggta
      781 tcaccaacgt tcgatacata ttccctgctc cttacttttt gcctatactg ctgatgattt
      841 ctttcagatg tccgatcatg tcatcgtgaa atttgtcaat gacatctata aaatcttcct
      901 gctttcgttc tgcaacaaat tccggttcgc taacgccggt caccaacaca gcgggcgttt
      961 ggacacccat ttcgcgtaat ttacgcaagg ttcgcgatcc gttattttcg tgatcgtcaa
     1021 aatcgaaatg aaaatcgagc aggatcaggt cgggtggaga atttttcgcg tatgctgcaa
     1081 taagactatc tccatccgga aaggttttta cttctgctcc caatcccatc cggagttctt
     1141 gtgtaataag ggttctgtat accggcgaat catcggccag ccagattttg atcttgttgc
     1201 tttccactgc acgttgcttt tcacggcaaa gttcattata tttatcagaa tgaccttatt
     1261 ctcctcctat gaataaatgg atagtcccta gtatattgag tctgagtctt cttgtttcct
     1321 gcgctaagcc aaaatacagg tcggaagagg ggggatttgc cgtttcattc aaaggaattc
     1381 ctcaggaaaa caccgaaaat gtagctactg ctcatggcga tattgctgtg accatgttcc
     1441 ttgatgaggt tagtcctgat atggtttatc tggtcggcta ttccgattat ccggaagcgg
     1501 ttttagcagc aaccagtcct gaagctcacc tcatgaaaac ggcagagaat gttgctgaaa
     1561 gcgtaaaggg tacactttct gatgtaaaaa agaactccgt aaatgggttt ccgtcactcg
     1621 atttcaggtc ggtggggaat ccgttttctg ttgcctaccg tctcataatc aggagcacac
     1681 gcatgtatca ggtgggtatc atgaagcaaa aagaggcaat aaaggattct gaaattcaag
     1741 gttttataga aacatttgaa atattggaat aagttgtttg cgggttacaa tggaaaatct
     1801 atctttgcaa cccgttttta agcgatttct ataacacaaa agcataccga tcatgaaaag
     1861 gacattccaa ccctcgctcc gtaaaagaag aaacaaacac ggtttccgtg agcgcatggc
     1921 ttcacaaaac ggacgtcgtg tccttgctgc gcgccgcaaa agaggccgtc ataaacttac
     1981 tgtatcaagc gaaaagcgtc acaagcgtta atctctgaac taatattttt cagaaacccc
     2041 ggccttaata ggttggggtt ttttattact ggtgaacggt gaacagtaat cggtgaacag
     2101 tgaatggtga atggtgaaca gtaatcagtg atcccgacgc tatcggtcgg gacaggccgg
     2161 tgaaaagtga atggtaactc ctaactccta actcttaact gataaccaat aaccgataac
     2221 cgataaccga tatccaatac ctactcagct aatttgattt cgttttacag tcgctgcaga
     2281 ggagtacatt ctccatttac acaggagtag cacttgagtt tgtctttgaa tacgctggat
     2341 ttgaaaaccg atttgttcaa aagcatacgt acacattcct gaacaccttc aatgatggac
     2401 gggtgggggt gaatcatttc tgcaagctca tcaataccct tacccatttt gatcaatagg
     2461 gcacaagcct gaatcgctga agatgcgtgt tcaccaatag cgcgcattcc tatgatttta
     2521 ttttcgttat cgttcgtaac aatgatcttg aaaaaccctt gtgttttacg catggcaatg
     2581 gctcgggcaa tgacggaata atcgactttc accaccttaa cgggaatgtt cttctcacgg
     2641 cattgctttt cattcattcc cactgctgca acttcgggat gaaggaacat aatggttgag
     2701 atattgtcgt aggaaagcgg attgaccata tcgtctccca tcgaaatttt ttcgactgca
     2761 tgacgtgctt caagttctcc tacgttaacc aattgaatgc gtcctgatac atcgccaaca
     2821 gcataaatat gcggaacact ggtgcgggta tctacatcgc caatgtggac tcctcttttc
     2881 gacatgtgta caccggcttc gttcaagccc agttcttcaa tattaggcac acgtccgata
     2941 gcaagcagtg ctttctccac acgaatcact tcacggcgac catcggaata ggagagttcg
     3001 tattccactt ctccatcaac attaaccaat cgttccagtt gcgcgttgtg gtgaatggta
     3061 acgcctttct cttcgagatt gtgcgaaaca agatcagaaa tatcttcatc ctcaaaaggc
     3121 agaatgcgat cagcacggtc gatgatatac actttggttt ttccgaaatt cgcaaaaatg
     3181 gtagcgaact cacaaccaat tacaccggca ccgacaatta ccagactctt ggggaaatcg
     3241 gtcatgtgat gaatgccgtc ggatgtcata attacttttt catcgacagt tacattggga
     3301 agtttgcgtg gacggcttcc cgttgccaga atgatattat ccccccatac cactttttcc
     3361 tcgtcctctt tacggattag aatttcattc ggagagatca ttgttcctgt tccgcgttcg
     3421 taatagaaca aacggttctc aacttctgtc tgtaaaagtt taatatgaca ggaatattga
     3481 aacttacgat cgaaaatagc ttcgttcatt gtacgaatta cttct
//
LOCUS       DAOMYG010000342         3522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9578_length_3522_cov_7.692530, whole genome shotgun sequence.
ACCESSION   DAOMYG010000342 DAOMYG010000000
VERSION     DAOMYG010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9578_length_3522_cov_7.692530"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..2065)
                     /locus_tag="PK610_10630"
     CDS             complement(29..2065)
                     /locus_tag="PK610_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36435.1"
                     /translation="MSIPTVLLCIGVNAQSIRQEVKTLTEYWNQYLPKVQSPAVKPNL
                     AKAEGLFHSGLNPSAGISADDEVDRIRARMYKTDPGLSASASYLENFTPSFNEDDDNL
                     IYQRRLQAGINWDILEGGFYQNRIRSEQLNNSIAIRRFLEANNWKEDDYSLRWNQIIY
                     VFNKSKIATLEKRRDLIESLSTEAEKLHLSRYLTREDYLRILSRKAEIDALLNIYYDY
                     NQQFSASADSLKINADELPVPDIRYIEVLSGLDSSSRDSIGRLVARNIQLENKLISDV
                     RLSTQLRYNYYDLVIPGNRSFFSLGVNLSIPIPFQMNLRGELNEARTKQSMYHIDQQL
                     RGKEKELLNECYEYRYKLKQYVGFHQKFLLYEELIRRLNATSNIEPERFNPIEGLLLL
                     DDMLAVQMEMTDIRQSLYLKLLRMYVRTGTSDIAGIAEVFSLPNYFDFDTFTNRSGYL
                     WSAGLAKRSAQFISEYLVYNKFDKLIVSLNTDKEHKNRLVQLLPLVKKNNIDVELMIG
                     DNELIRKTGSVATELENLCKGLTDSTINGIHLDVEPHTFSDWETEKAAYMNSYLQMLN
                     EVRNFCNQRKWKLSVSIPLHYPEETVNKIFSICDQVIFMAYENIKPEYIDRKLLPYAG
                     QKVKLAVAVRTKDFQNRIQIEKYLIDLYHRTGIEQIAIHDIETLISMDEKNLER"
     gene            2190..3476
                     /locus_tag="PK610_10635"
     CDS             2190..3476
                     /locus_tag="PK610_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36436.1"
                     /translation="MNQNTSQGGKRWLIQLCLLGTLLFSLVVLGYFANRSIFWTIGIP
                     FLIGGLAYYMIAINGQYRLRSPGAILLTGLVLRLVFLLAEPRLTEDHYRFFWDAKVSL
                     SESSKGVYGSTPGELMSDGSKVAIENRDLYCVLNSPDYPTIYPPLAQVFFVSATITGK
                     SYFGTLISFRVFLILSELILVFVLLRLFRKFKIEPSGIAVWVFNPVVIVEFTGNLHFD
                     GLMILFLVASIGFLLEKKWIPAAILLSFSVGIKWLTLIYFPLLLTVAGWRKTMWSGLI
                     ACGTIAILFVPFIRGNDMNYLLDSFGLFLQKFEFNASIHYLTSAVGKNLVGFNPLYYS
                     GIFFFCLVCCILAFFAMRKPKDSNGINYFIAAGGTVYFLLLLFSSVVHPWYVVLLLIP
                     AMFLDWKFPIVWSLLIPLSYIFYDGEIHFLYPVFAR"
BASE COUNT          879 a          692 c          753 g         1198 t
ORIGIN      
        1 ggttttccgc gttgaaaatt gatgtttttc atcgctcaag gtttttttcg tccatgctga
       61 tcagggtctc aatatcatga atggcaatct gctcaatacc ggtgcggtgg taaagatcga
      121 tcaggtattt ttcaatttga attctgttct gaaaatcttt tgtgcgaact gcaactgcca
      181 gcttcacttt ttgtccggca taaggaagta gttttctgtc gatgtactca ggcttgatat
      241 tttcatacgc cataaaaatg acctgatcgc aaatggaaaa tattttgttg actgtttctt
      301 cgggataatg caatgggatg gagacactga gtttccattt gcgctgattg cagaaattgc
      361 gcacttcgtt tagcatttgc aggtaactat tcatgtaggc tgccttttct gtttcccaat
      421 cggaaaaggt gtgtggctca acatccaggt gaattccatt gattgtggag tcggttagac
      481 ccttgcataa attttccaac tctgtggcaa cagatcctgt tttacgaatc aattcgttgt
      541 cgccgatcat caactcaacg tcgatattgt ttttcttaac cagtggaagg agttgaacta
      601 gtcggttttt atgctctttg tctgtattga gcgatacaat gagtttgtcg aacttgttgt
      661 aaacaaggta ctctgagatg aactgagcag accgttttgc taaaccggcc gaccacaaat
      721 agccgcttcg gttggtaaat gtatcgaagt cgaaataatt cggaagcgaa aatacttctg
      781 caattcctgc aatatcagat gtgccggttc ttacatacat tcgaaggagt ttaagataaa
      841 gactctgacg gatatctgtc atctccattt gtacagcgag catgtcgtcg agcaacaaca
      901 agccttcgat cggattgaat cgctccggtt caatgttgct ggttgcatta aggcgacgaa
      961 tcagttcttc atacaacaaa aatttttgat ggaagcccac atattgtttg agcttatagc
     1021 ggtattcata acattcattc agcagctctt tttccttacc acgtaattgc tggtcgatgt
     1081 ggtacatcga ttgtttggtt ctggcctcgt ttaattctcc ccgcaaattc atttggaatg
     1141 gaataggaat gctgagattt actcccagcg aaaagaaact tcggtttccg ggtattacca
     1201 gatcataata gttgtatcgc agttgagtgg ataagcgaac atcgctgatc agtttgttct
     1261 ccaattggat atttcgggca acaagccggc caatactatc ccgcgaactg gagtcgagac
     1321 ctgatagtac ttcaatatag cgaatgtcag gtaccgggag ctcgtctgca tttattttca
     1381 gtgaatctgc cgatgcactg aattgctgat tatagtcgta gtaaatattg agcaatgcat
     1441 cgatctctgc ttttcttgaa agaatgcgaa gataatcttc gcgtgttagg tatcttgata
     1501 gatgtagttt ttcagcttcc gtacttaatg attcaataag atcgcgacgt ttttcaagtg
     1561 tggcaatttt acttttgtta aatacataaa tgatctggtt ccaccgtaaa ctgtaatcgt
     1621 cttctttcca gttattggct tccagaaagc gacgtattgc gatggaattg ttgagttgct
     1681 cgctgcgtat tctattctgg taaaaaccac cttcaaggat atcccagtta atccctgcct
     1741 gcagtcttct ctggtaaatg agattgtcat catcttcatt gaaagaaggt gtgaaatttt
     1801 ccaggtaaga agcacttgca gataatcctg gatctgtttt gtacattctc gctctgatcc
     1861 tatccacttc atcatctgca gaaattcctg cacttggatt taaaccacta tgaaaaagac
     1921 cctcggcttt tgctaaattg ggctttacgg cgggactctg tactttgggc agatactgat
     1981 tccagtattc agtcaatgtt tttacctcct gacgaatgct ttgcgcgttc acaccaatgc
     2041 aaagcagtac tgtcggaatt gacaatagcc gtggaataat tgaaagaaat gtaatcgctg
     2101 ttggcacaag tagtaaaacg gggggcaaaa ataatgcctt tttatcgata aaacactgat
     2161 tttatgggaa aaaccttcat ttttacagga tgaatcaaaa cacttctcaa ggtggaaaaa
     2221 gatggctcat ccagctttgc ttgctgggta ctttgctgtt ttcccttgta gtgttgggct
     2281 atttcgcaaa cagaagtata ttttggacga tcggaattcc gtttttgatt gggggattgg
     2341 cctattacat gattgccata aatggtcaat accggctgcg aagtccgggt gcaattctac
     2401 taaccggatt agtcctgcgc ctggtatttt tgcttgcaga acctcgtttg accgaagacc
     2461 attatcgttt tttttgggat gcaaaagtta gtttgtcgga atcttcgaaa ggcgtttatg
     2521 gttctactcc tggagaattg atgtcggacg gcagcaaagt ggcaatagaa aaccgcgatt
     2581 tgtactgtgt tttgaattct cccgattatc ctaccattta ccccccgctt gcacaggtgt
     2641 tttttgttag cgcaacaata accggaaaat cgtatttcgg aacattgatc agttttcggg
     2701 ttttcctgat tctttcagaa ttgatattag tatttgttct gcttcgtcta ttcagaaaat
     2761 ttaaaattga accctcaggt attgctgtct gggtgttcaa tcccgtggtc attgttgagt
     2821 ttacaggaaa cctgcatttc gatggattaa tgatactttt tctggttgct tccattggtt
     2881 ttcttctcga aaaaaaatgg ataccggcag caatcctgct cagcttttct gtcggaataa
     2941 aatggttaac cctgatttat tttccactgt tgctgactgt tgctggatgg agaaaaacaa
     3001 tgtggtccgg actcattgcc tgcggtacaa tagcaattct atttgtgcct tttatacgag
     3061 ggaatgacat gaattatctc cttgattcat ttggattgtt ccttcaaaaa ttcgagttca
     3121 atgcttccat ccattacctg accagcgcag ttggaaaaaa cctggttgga ttcaatccct
     3181 tgtattattc cggaatcttt ttcttttgtt tggtttgttg cattcttgct ttttttgcaa
     3241 tgcgtaaacc taaggatagc aatggaataa attatttcat tgccgccggt ggaacagtct
     3301 attttttact actgcttttt tcttcggttg ttcacccctg gtatgttgtt ttattgctga
     3361 ttccggctat gtttctcgat tggaaatttc caattgtttg gagtttattg atccctctga
     3421 gttacatttt ttacgacggt gaaattcatt ttctctatcc tgtttttgct cggtgagcgt
     3481 ttcggtgttg gcgaccaacc gtttgggctt ctggaatcct tt
//
LOCUS       DAOMYG010000343         3519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9595_length_3519_cov_10.177540, whole genome shotgun sequence.
ACCESSION   DAOMYG010000343 DAOMYG010000000
VERSION     DAOMYG010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9595_length_3519_cov_10.177540"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..472)
                     /locus_tag="PK610_10640"
     CDS             complement(23..472)
                     /locus_tag="PK610_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRJ36437.1"
                     /translation="MKLEDEIKQKKFSSEFHKAQINILFTASWVNGQVIPVMKPYGIS
                     SQQFNIMRILRGQHPNPAPLKLLTERMIDKMSNTSRLVEKLVQKKLVERKICKDNRRQ
                     IDISLTEKGMDVINKISDDMEKFFKRKQEITPEEAKELNRILDKLRG"
     gene            complement(581..2548)
                     /locus_tag="PK610_10645"
     CDS             complement(581..2548)
                     /locus_tag="PK610_10645"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRJ36438.1"
                     /translation="MKQTKAKWMGVAVMASLLVSSCKYETSNVTGWNYNDPNNGGYQK
                     VPYEEQETGPGLILVEGGTFTMGRIEQDVYHDWSNVPRRVTVSSFYMDETEVTNFNWC
                     EYLYWLRRVYTDFGQIYTNALPDTLVWREKMAYNEPYVEYYLRHPAYREYPVVGVSWL
                     QATEFCAWRTDRVNEYILIREGVLNWDVTFQPNDPFTTDEYLDQPGGVPHTNGVENLL
                     VDLNKQSNPGPGKKGKKALGRRHVRMEDGILLPRYRLPTEAEWEYAAYGLIGNSVEER
                     IVERRIYAWNGHWVRNPESKWQGEMLANFVRGRGDYMGVAGKLNDAADITAPVTSYWP
                     NDYGLYNMAGNVSEWVMDVYRPLTSEDADEFRPFRGNVFKTKVLNAEGIIDNKHDETI
                     YDVDAMRIYLDSFHLARSNTGYLTDIEDSLLKYLKTEIVDSAISVKNSYRKSYQKRAN
                     LVRENEQLIQASIIIREMFDAALVDFEVNLRADPRYENYQIEIIPVLRVKLSDFVINT
                     PGNVKWRQVTEEENIDRRTYRKSDNIDYLDGDIASTQYYGTAGHSKSGAVISEVNSDT
                     RWQENIMYQSRYEGVNRHDNSASPTTLVSNRTRVYKGGSWRDRAYWMNPGTRRYLDER
                     RSTATIGFRCAMDRLGSPIGIGGKKKKKKKK"
     gene            complement(2634..>3519)
                     /locus_tag="PK610_10650"
     CDS             complement(2634..>3519)
                     /locus_tag="PK610_10650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRJ36439.1"
                     /translation="TQFFANPLYLNPAFAGSKRCPRINLNYRNQWPALSGTFVTTSAS
                     YDQHVESIYGGLGLLVTNDKAGEGTLNTTTINAMYAYQLKLGRKFSLRFGVQASYFQK
                     SLDWSKLTFGDMIDPRRGFVYQTGDVPRGGSVGNMDFSAGILGFSENFYIGFSAHHLT
                     EPNESLVTGTSPLPMKLSGHAGAVIPLGNSKYSTNDATLSPNIMYRSQGTFQQLNMGL
                     YVNTKNIVFGAWYRNKDAFIALIGVKSDIFNFGYSYDVTVSKLTNASGGSHEISMAIN
                     IPCKPKKRSFRTISCPSF"
BASE COUNT          924 a          794 c          784 g         1017 t
ORIGIN      
        1 gttccgtttg cgatttatct gtttatcctc tgagtttatc cagaatacga ttcaattctt
       61 tggcttcttc cggggtgatc tcttgtttgc gcttgaagaa tttttccata tcatcggaga
      121 tcttgttaat gacatccatc cctttttcgg tgagcgaaat gtcgatttgt cgacgattat
      181 ccttgcagat tttccgttca acgagtttct tctgaaccaa tttctcaacc agtcgtgagg
      241 tgttcgacat tttgtcgatc attcgctcag tgagcagttt aagcggggca gggttagggt
      301 gctgacctcg cagaatacgc ataatgttga actgctgact ggaaatcccg taaggtttca
      361 ttactggaat tacctgtccg tttacccagg atgcagtaaa aagaatgttg atctgtgcct
      421 tgtgaaattc cgagctgaat ttcttttgtt tgatttcgtc ttcgagtttc atgctgcaaa
      481 tatagttaaa caaaatacac ttgtattagt tgttgataca agtcattttt tgcaaaagaa
      541 aaagtcccgc cttagcggga ctttatttga gagaagatga ttacttcttt ttcttctttt
      601 tctttttgcc accgatgccg atggggcttc ccaggcgatc cattgcacaa cggaaaccga
      661 tagtggccgt agagcgacgc tcgtcgaggt aacgacgggt accggggttc atccaatagg
      721 cacggtctct ccagctaccg cctttgtaaa cacgagtacg gttacttacc agtgtagttg
      781 gtgatgcaga gttatcgtga cggttaacac cttcgtatct gctctgatac atgatgtttt
      841 cctgccagcg ggtatcgctg ttcacttcgc tgataacggc acctgactta ctgtgacctg
      901 cagtaccata gtattgggta gaggcaatat caccatcgag gtaatcgatg ttgtcggatt
      961 tacggtaagt acgacggtcg atgttttctt cttccgtaac ctgacgccat ttaacgttac
     1021 ccggagtatt gattacgaaa tcagaaagtt tcacacgcaa tacagggatg atctcgatct
     1081 ggtagttctc atagcgtgga tcggcacgga ggttaacttc aaaatcaacc agtgcagcat
     1141 caaacatctc acggatgata atagatgcct gaatcaattg ctcgttttca cgaaccagat
     1201 tcgctcgctt ttgatacgac ttacggtaac tgtttttcac agaaatagct gagtcaacaa
     1261 tctcggtctt cagatactta agaagtgaat cttcaatatc agtcaggtaa ccggtgtttg
     1321 aacgggcaag gtggaacgaa tcgaggtaaa tgcgcattgc atcaacatca tatattgttt
     1381 catcatgctt attgtcgatg ataccttctg cattcaaaac tttcgtttta aatacgttac
     1441 cacggaaggg acggaactca tctgcatctt cggaagttaa aggacgatat acatccatta
     1501 cccactcaga aacgttacct gccatattgt acagaccata atcattaggc cagtaagagg
     1561 taacaggggc agtaatatct gcagcatcgt tcagttttcc ggcaacaccc atatagtcac
     1621 cacgtccgcg cacgaagttg gcaagcattt caccctgcca tttggattcc gggttacgaa
     1681 cccagtgacc attccatgca taaatacgac gctcaacgat acgctcttct actgaatttc
     1741 caatcagacc gtaagcagca tattcccact ctgcttctgt tggcaggcgg taacgtggaa
     1801 gaaggatacc gtcttccata cgaacgtgac gacgtcctaa tgctttttta cctttcttac
     1861 ctgggcctgg attcgattgc ttgttaaggt caaccagcag gttctcaaca ccattggtat
     1921 gcggcacacc accgggttgg tcgaggtatt cgtctgttgt gaacgggtcg ttcggctgga
     1981 atgtaacatc ccagtttaaa acgccttcac gaatgagtat gtactcgttc acacggtctg
     2041 ttctccaggc acagaattca gtagcctgta gccagctcac acctaccaca ggatactcgc
     2101 ggtatgcggg gtgacgaaga taatattcta catagggctc attataagcc attttctcac
     2161 gccaaaccag tgtatcgggt agggcgttgg tgtagatctg accaaaatcg gtataaacac
     2221 ggcgcaacca atacaggtac tcgcaccagt tgaaattggt tacctctgtt tcatccatat
     2281 aaaaggaaga aaccgttacc ctgcgtggaa cattgctcca gtcgtggtaa acatcctgtt
     2341 caatacggcc catggtaaag gtacctccct ccacgaggat caatcccgga cccgtttctt
     2401 gctcttcgta aggaaccttc tgataaccac cattgttggg atcgttgtaa ttccaaccgg
     2461 taacattaga agtttcgtac ttacacgaag agaccagtag acttgccata acggcaactc
     2521 ccatccactt tgctttcgtc tgcttcattg acatatctgg tttatttact cctgcttact
     2581 taattctcct ttataacgta aatctgaaaa atggttacgt ttattcttgt tttttagaac
     2641 gatggacagc ttattgtcct gaaagaccgc ttcttaggtt tacagggaat attgatggcc
     2701 atcgaaattt catgcgatcc gcccgacgca ttggtcagtt tggaaacggt aacatcgtag
     2761 ctgtacccga aattgaagat gtctgatttc acaccgatca gtgcaatgaa ggcatcttta
     2821 ttacggtacc aggctccgaa cacaatgttc ttggtgttaa cataaagtcc catgttcagc
     2881 tgctggaatg ttccctgcga gcgatacata atgttgggag aaagagttgc atcgttggtt
     2941 gaatactttg aattccctaa tggaattaca gcaccggcat gacctgaaag cttcattggt
     3001 aatggactgg ttcctgtaac gagtgattcg ttgggttctg ttaagtggtg cgcagaaaat
     3061 ccgatataga agttttcgct gaaaccaaga atacctgcag agaaatccat attgccaaca
     3121 gatccgccac ggggaacatc acctgtctga taaacgaaac cacgacgcgg gtcgatcatg
     3181 tcgccaaaag tcagcttgct ccagtcgagt gatttttgaa aataagaagc ctgtactcca
     3241 aaacgcagtg agaatttgcg tccgagcttt aattgatagg catacattgc attaatagtg
     3301 gtagtattca aagttccttc accggctttg tcgttggtaa ccaaaagacc aagtccacca
     3361 taaatagact caacatgctg atcataagaa gctgaggtgg taacgaatgt cccggatagg
     3421 gcaggccatt ggtttctgta attaagattg atacgcggac aacgcttgga tcccgcgaag
     3481 gctgggttca aatatagcgg gttggcgaag aattgcgtg
//
LOCUS       DAOMYG010000344         3505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9660_length_3505_cov_8.947536, whole genome shotgun sequence.
ACCESSION   DAOMYG010000344 DAOMYG010000000
VERSION     DAOMYG010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9660_length_3505_cov_8.947536"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="PK610_10655"
     CDS             <1..1160
                     /locus_tag="PK610_10655"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HRJ36440.1"
                     /translation="ADGNVFIDYCCSWGPLILGHAPDEVVEAVKTSVEKGSSFGAPTS
                     LENDLADLILSNNPFIEKIRFVSSGTEAVMSAIRLARGVTGRSKILKFEGCYHGHVDS
                     LLVKAGSGLVTFGNTSSAGVPESFVNETIVVPLDNEKAVEEAFVKFSNEIACVIIEPI
                     PANNGLLLQRKEFLQFLREICDKNNSLLLFDEVISGFRVGFTGAAGLFGMRPDIITYG
                     KIIGGGLPVGAYGASAAIMGNISPEGGVYQAGTLSGNPVAMSAGIAQLTECLKPGFYV
                     DLERKTKYLIDEILRLTPAELHDRFKFFSVGSIFWIAFTSENAIRSAEAIDADSMSYF
                     RVLHKELLERGIYLGPSGYEVGFVSDAHSVEDLRITAKAFAETLPIALAQE"
     gene            complement(1171..1596)
                     /locus_tag="PK610_10660"
     CDS             complement(1171..1596)
                     /locus_tag="PK610_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36441.1"
                     /translation="MNKSIIIAACWVYLCVFIPAKNGTVEFNALPLIQIFLLINATAL
                     VFDIRDMEQDTELHRSIPGIFGPKNSLILAYFSILATLVLGIVFHHTPFAIASAAVFV
                     SGLVLFGFRNLFEKELLYTAGVDGLLIIHPLVLFACTDF"
     gene            complement(1980..2654)
                     /locus_tag="PK610_10665"
     CDS             complement(1980..2654)
                     /locus_tag="PK610_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36442.1"
                     /translation="MRSYYLTLFLLLFSPLLVSAQDKLTFLNGHVLEGKFLNYDTSYV
                     EFSYQRRNKTKTEKFEAYRLYSITEASGKETVVYFQDSLAGNIWTPQEAMYFVHGQQD
                     AQEGFKPHLANIGGFSFALGVSLWDTYKRQNGPSDGFFEGFFRSDPGIIHLLTPFVYT
                     ILVGLPGITFDLSKVSNRAYLLEETYRDGYIRVVKNKRKFGGLKFSLLGSATGLGLYF
                     LGRAIQ"
     gene            2788..>3505
                     /locus_tag="PK610_10670"
     CDS             2788..>3505
                     /locus_tag="PK610_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HRJ36443.1"
                     /translation="MKTDRFSDRHIGPSSSETEKMLRKIGVGSLNELIEQTVPNAIRR
                     KSELNVGAALTEFDYLNHLRKVGAKNKVLRSYIGMGYYGTILPSVIQRNILENPGWYT
                     AYTPYQAEIAQGRLEAILNFQTMVIDLTGMPIANASLLDEGTAAAEAMAMLFSDRDRD
                     TVKRGANVFFVSDEVYPQTIDILRTRAIPLGIELQIGNFRELKFTDKIFGSVLQYPAL
                     NGEVYDYADFVKQAKANKTAV"
BASE COUNT         1011 a          720 c          793 g          981 t
ORIGIN      
        1 atgcagatgg aaatgtattc attgattatt gctgcagctg gggcccgctg attctcggac
       61 atgcgccgga tgaagtagtg gaggctgtaa aaacttcggt tgaaaaggga agttccttcg
      121 gtgcgcctac ttcattggaa aatgatctcg ccgatctgat cttgtcgaat aatcccttca
      181 tcgaaaaaat tagattcgta tcctccggta ccgaagctgt aatgagtgca attcgccttg
      241 ctcgtggagt aaccggtcga tctaaaattc tgaagtttga aggttgctac catggccatg
      301 tcgactccct cctggttaaa gcgggttccg gattggttac cttcggaaat acttcttctg
      361 cgggtgttcc ggagtcgttt gtgaatgaaa cgatcgtggt tccactcgac aatgaaaaag
      421 cagttgaaga ggcgtttgta aagttcagca acgaaattgc atgcgttatc attgagccta
      481 ttcctgccaa taatggatta ttgcttcaac gtaaagagtt tttgcaattt ctcagagaga
      541 tttgcgataa aaacaattcc cttctccttt ttgatgaggt aatatcagga ttcagagttg
      601 gatttacagg cgctgcagga ttgttcggta tgcgtcccga tattattacc tacggtaaaa
      661 ttattggagg aggattgcca gttggtgctt atggtgcatc tgctgcgatc atgggaaaca
      721 tttcgccgga aggtggagtt tatcaggccg gaaccttatc tggtaatcct gttgccatga
      781 gtgcaggtat agctcaactt accgagtgtt taaaaccggg attttatgtc gacctcgaac
      841 gtaagacgaa gtatttgatc gacgaaattc tccgactgac tccagcagaa ttgcatgacc
      901 gttttaaatt tttctctgtg ggttcaattt tctggattgc gttcacctca gaaaatgcaa
      961 ttcgcagcgc ggaagcaatt gatgccgata gcatgagtta tttccgtgtg ctccataaag
     1021 aattacttga acgcggaatc taccttggtc ctagtggata cgaagtggga tttgtaagcg
     1081 atgctcacag tgtagaggat ctgcgaatca ccgcaaaagc atttgccgaa actcttccaa
     1141 tagctcttgc tcaggaataa gttgcaattt ttaaaaatcg gtgcaggcaa acaaaaccaa
     1201 tggatgaatg atcaataatc catcaacgcc tgcagtgtat agtagttctt tttcgaatag
     1261 attgcgaaaa ccgaaaagga ccaatcccga aacaaaaact gcagcagatg ctattgcaaa
     1321 gggggtgtgg tgaaatacaa ttcctaaaac aagcgtcgct aaaattgaaa agtatgctag
     1381 gatcagactg tttttaggac caaaaattcc cggaatgctc ctgtgtagtt cggtgtcttg
     1441 ttccatatcc ctaatgtcaa aaaccaatgc tgttgcattt atcaacagga aaatttggat
     1501 cagcggtagc gcattaaatt ctacggttcc gttttttgcc gggataaaaa cacagagata
     1561 aacccaacat gcagcaatga taatactctt gttcaaatga atttgatccg gtttaatgag
     1621 tattccggat cggaagagtg tatataacaa tgagactatc gcaatgggaa ggaaccaaag
     1681 atggagttcg gggaatttga aaataaaaag aatgaaactt gtgatgcctg caatgatcat
     1741 acaggcgatc aattcgattg gatgctttcg tatccattgt tgccgttcgc tttcaaaggg
     1801 caattgtttg cggtgtttta caaaacggtg aaaattgtaa atgaaaagtg taccgcaaaa
     1861 aatcaaaact ccgtagagca gggggctgct tattgaaaaa aacgaagccc aataccacga
     1921 ttgtaaaccg gctgctaacg caatccagcc attgagataa agaaaaacac gaataagtat
     1981 cactgtatcg ccctacccag aaaatagagt cctaatcctg tagcgctgcc caacagactg
     2041 aattttagtc cgccaaattt ccgtttgttt tttaccacac ggatataacc atcccgatac
     2101 gtttcttcca gtaaataggc acggttgctg actttcgaca gatcaaaggt tattccgggt
     2161 aagccaacaa gaatggtgta aacaaaggga gtgagcaaat ggatgattcc cggatcgctg
     2221 cgaaaaaaac cttcaaaaaa accatcgctt ggtccgttct gccttttgta tgtgtcccat
     2281 aacgaaacac caagtgcaaa actgaatcct ccaatgttgg ctagatgggg tttgaaacct
     2341 tcctgggcat cttgctgacc atgaacaaaa tacattgcct cttgaggtgt ccagatattt
     2401 cctgcgagtg aatcctgaaa atagacaacg gtttcctttc cggacgcttc ggtaatgctg
     2461 taaagacgat aggcctcgaa cttttcggtc ttggttttgt ttcttctttg gtagctgaac
     2521 tcaacatagg aagtgtcgta attcaaaaac ttgccttcca gtacgtgacc gttcagaaaa
     2581 gtaagtttgt cctgcgctga aaccaggagc ggagaaaaaa gcaacagaaa caatgtcagg
     2641 tagtaagacc tcatttttga gaaggatttc ggtgacaatg tacagctttt ttagcgcaag
     2701 tccactattc accgctgttg aaaactgtcg ccctttctgc gttcttttct ggttatattt
     2761 gcccctcttt cgaactccat tttccttatg aaaacagacc gtttttccga ccgacatatc
     2821 ggtccttcgt cttccgagac tgaaaaaatg ctccgcaaaa tcggggtagg ctcattgaac
     2881 gaactcattg agcaaactgt tcctaacgct atccgacgca aatcggaatt aaatgttggt
     2941 gcagcattaa cggagtttga ttacctgaat cacctgcgca aggtcggagc aaaaaacaag
     3001 gtgcttcgtt cgtatatcgg catggggtat tatggaacaa tacttccatc cgtaattcaa
     3061 cgcaatattc tggagaatcc gggttggtac acggcatata ctccgtacca ggcagaaatt
     3121 gcacaaggtc gacttgaggc tattctgaat tttcagacga tggtcattga tcttaccgga
     3181 atgccaattg caaatgcttc tctgctggat gaaggtactg ctgctgccga agcaatggca
     3241 atgctctttt ctgatcgcga ccgtgataca gtaaagcgtg gtgccaatgt gttttttgtc
     3301 agcgatgagg tttatcctca aaccatcgat atccttcgta cgcgggctat tcctttggga
     3361 atagaactgc agattggaaa tttccgcgaa ctgaaattca cagataaaat tttcggatcg
     3421 gtattacagt atcctgccct caatggtgaa gtgtatgatt atgctgattt tgtaaaacag
     3481 gcaaaagcaa acaaaacagc tgttg
//
LOCUS       DAOMYG010000345         3494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9695_length_3494_cov_7.103809, whole genome shotgun sequence.
ACCESSION   DAOMYG010000345 DAOMYG010000000
VERSION     DAOMYG010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9695_length_3494_cov_7.103809"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PK610_10675"
     CDS             <1..199
                     /locus_tag="PK610_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36444.1"
                     /translation="DNLSINYIADALMELFPGLEMIFVNQHMTLRDLRVKPNPIVNDQ
                     IDIPVRTLKEVLGAFKEAFTF"
     gene            complement(202..993)
                     /locus_tag="PK610_10680"
     CDS             complement(202..993)
                     /locus_tag="PK610_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRJ36445.1"
                     /translation="MTDIRARHRFLEKFRLNSVLSAVLFLVLGMANAQDVTRKLQMQY
                     PRVKEAFSEKWDALKKDIQAMGMETSGFNVLLCGFKAEKKLEVYLSSKGERTFRHFKT
                     YDFCTLSGELGPKRKQGDYQIPEGFYHIDRFNPSSNFHLSLGINYPNASDKLLSDKKS
                     PGGDIFIHGNCVSVGCVPITDDLIKELYVLCVEASEGGQKKIPVYLFPARMTETAMES
                     MRKMYSLNAGLLDFWNNLKEGYDLWDKNKQPLKWKVNEKGKYLFQ"
     gene            1180..2784
                     /locus_tag="PK610_10685"
     CDS             1180..2784
                     /locus_tag="PK610_10685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRJ36446.1"
                     /translation="MKRIVITLLLLSGALMPDISRAQHNNRSATTDKFNMLMNYISQM
                     YVDSVKEDELTEAAIIHMLEMLDPHSIYIPKKEVEEMNAPLKGGFDGIGVRFQIMKDT
                     VMIVNTIPGGPSEKLGIRAGDKIIKVEGENIAGAGIKNYQVREKLLGLKGTKVQVEIQ
                     RRGEKNLIKYTITRDKIPLYSVDASYMATPTTGYIKLNSFGENTMAEFTRAFDSLKTL
                     GMQNLIFDLQGNGGGYLNTAIELCDEFLSGSKLIVYTEGRSFPRKDTYARKVGRFETG
                     KLILLIDESSASASEILSGAIQDWDRGLIVGRRSFGKGLVQKPMNLPDGSQVRLTTQR
                     YFTPTGRCIQKPYDDGSEAYRKEKYQRYLSGEMLSLDSIKIPDSLHFETRINKRTVYG
                     AGGIIPDIFVPLDTTRSSEYLSNLLRNGVFNNFCLVYVDNNRKLLKKNYPDFQKFKKG
                     FAVDQKLLDEFTAFAEKEGVKFEATGFSTSRAVIETRIKAGIAQNIWDAGQFYELVNP
                     LNPIYLRALEALEDDTFDKLKLAYKP"
     gene            2826..3404
                     /locus_tag="PK610_10690"
     CDS             2826..3404
                     /locus_tag="PK610_10690"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRJ36447.1"
                     /translation="MNSETRETAKLGLLGVIALTLIVNTYVVATKKSGGSYVPQPATS
                     AAVATPAGGVPTDNPMGNPNPNLSTTINPADPTTSPIPVNPNQKNTRISFATYAHDFG
                     TVKQNSTNKFAFKFTNTGDEPLLITSAVGSCGCTVPNYPKDPIAPGKSATIDVEYKPG
                     LQKDAQEKKVTVTANTEPSQTILTIKANVIEE"
BASE COUNT         1043 a          844 c          696 g          911 t
ORIGIN      
        1 ggataatctg agcatcaatt atattgccga tgcattgatg gaactctttc ccggtctgga
       61 aatgattttt gtaaatcagc acatgacctt gcgcgatctc agagtaaaac ccaacccaat
      121 tgtaaacgat cagatcgaca ttccggttcg aacactcaaa gaagtacttg gagcgtttaa
      181 agaagcgttt acattctgag tttattgaaa aaggtatttc cccttttcat tgactttcca
      241 ttttaatggc tgtttgtttt tatcccacaa atcgtaaccc tctttcaggt tgttccagaa
      301 atcgagcaag cctgcattaa gcgaatacat tttacgcatc gattccattg cagtttccgt
      361 cattcgtgca ggaaaaagat aaaccggtat ttttttctgt ccgccctcag aggcctccac
      421 acaaagtaca tacaactcct tgatcaaatc gtcggtgata ggaacacaac ccacggagac
      481 acaattgccg tgaatgaaaa tatcaccacc gggcgatttt ttatcggaca ataatttatc
      541 ggatgcattc gggtaattga ttcctagcga caaatgaaaa ttactgcttg gattaaagcg
      601 gtcgatatgg taaaaccctt ccggaatctg gtaatcgcct tgtttgcgtt ttggtcccaa
      661 ctcaccggaa agcgtgcaaa aatcgtaggt cttgaaatgc ctgaatgttc tttctccttt
      721 ggaagaaagg tacacttcca attttttttc cgccttaaac ccgcacaaca aaacattaaa
      781 gcccgatgtt tccataccca tggcctgaat atcttttttc agggcatccc atttttcgct
      841 gaacgcttcc tttacccgcg ggtactgcat ttgtaattta cgggtaacgt cctgcgcatt
      901 tgccattccc aaaactaaaa agagaacagc actcagcaca gaatttaacc tgaacttttc
      961 taaaaaacga tgtcttgccc ttatgtctgt catagccact aatctacact gtcccttccc
     1021 ggtaaatgca ggaattgatt attgtaacgg atccgtctat tatttgttat ctgctgcaaa
     1081 ctttccatgc ttacctttat tccattcatc ctgccgttca tcccatctca tgatctcagg
     1141 tccgattttt gcgtatcttc aatctaaagt gctcaggcca tgaaaagaat tgtgatcacc
     1201 ctgctcctgt tatctggagc cctaatgccg gacatcagtc gtgcccagca taacaaccgt
     1261 tcagctacaa ccgacaaatt caacatgtta atgaattaca tctcgcagat gtatgttgac
     1321 agtgtgaagg aagacgaact aaccgaagcc gccattatcc acatgttgga gatgcttgat
     1381 ccgcacagca tttacattcc caaaaaagaa gtggaagaaa tgaatgcacc tcttaaagga
     1441 ggattcgatg gtattggagt tcgatttcag atcatgaaag ataccgtgat gatcgtaaac
     1501 accattcccg gcggtccctc cgaaaaactc ggtattcgtg caggtgataa gatcatcaaa
     1561 gtggaaggag aaaacattgc aggagcaggc atcaaaaatt atcaggtgcg tgaaaaactt
     1621 ctgggattaa aaggcaccaa agtccaggtc gaaattcaac gccgcggtga gaaaaacctc
     1681 atcaaataca ccattacccg cgataaaatt ccgctttaca gtgtggatgc ttcctatatg
     1741 gctacaccca caacgggata catcaagctc aatagttttg gcgaaaacac catggcagaa
     1801 tttacacgcg catttgattc gctgaaaacc ttaggcatgc agaacctcat tttcgatttg
     1861 caaggcaatg gtggtggtta cctgaataca gccattgagt tatgcgacga atttctgagt
     1921 ggaagtaaac tgattgttta tacggaagga cgttccttcc cccgcaagga tacgtatgcg
     1981 cgcaaggttg gacgctttga aaccggtaag ctgatccttc tcattgatga aagttcggca
     2041 tctgccagtg aaattctttc cggtgcgata caggattggg accgcggatt aattgtcggc
     2101 cgccgttcat ttggaaaagg actggttcaa aaacccatga atttgcccga tggttcgcag
     2161 gttcgcctaa ccacccagcg ttatttcacc ccaaccggac gctgcattca aaaaccatac
     2221 gacgatggat cagaagccta ccgcaaggaa aaataccagc gttacctcag tggagaaatg
     2281 ctgtcgcttg acagcataaa aatccccgac tctttgcatt tcgaaacacg catcaacaaa
     2341 agaaccgtat acggagcggg cggaatcatt cccgatatat ttgtcccgct cgacaccacc
     2401 cgcagttcgg aatacctgag caatctgttg cgcaacggag tgttcaataa tttctgtctg
     2461 gtgtacgtcg ataataaccg taaattattg aagaagaatt acccggattt ccagaagttc
     2521 aagaaaggct ttgcggtaga tcagaaactg ctcgacgagt tcacagcatt tgcagaaaaa
     2581 gaaggtgtaa aatttgaagc gacaggcttt agcacatccc gtgcagtgat cgaaacaagg
     2641 atcaaagccg gcattgctca aaacatctgg gatgcgggtc agttttacga actggttaat
     2701 cccttgaatc ctatttacct gcgtgcattg gaagccctgg aagacgacac cttcgacaaa
     2761 ttgaaattag cctacaagcc ctgatcaatt cccaattttc tgctattttt gaaaaaaaca
     2821 ataccatgaa ctcagaaaca agagagaccg caaaactagg attactgggt gtgattgccc
     2881 ttacgcttat tgtaaacacc tatgtagttg ctaccaaaaa atcaggtggt tcgtacgtac
     2941 ctcagcctgc caccagcgca gcagtagcta cacccgcagg aggagttccc actgacaatc
     3001 cgatgggaaa tccgaatccc aacctgtcta ctacaataaa ccctgcagat ccaaccacct
     3061 cacccattcc tgttaatccg aaccagaaga acacacgtat ttcatttgca acctatgcac
     3121 atgatttcgg aacggtgaaa cagaactcaa ccaacaaatt cgcatttaaa ttcaccaaca
     3181 ccggtgacga accattgttg attacttcag cggtaggttc atgcggatgt actgtcccaa
     3241 actacccgaa agatccaatt gctcccggca aatcagctac aattgatgta gaatacaagc
     3301 cgggtctgca aaaagatgcg caggagaaaa aagttacggt aactgccaac accgaacctt
     3361 cgcaaaccat ccttaccatt aaggcaaacg taatcgaaga ataatcacat tgaaataaat
     3421 cctcaagccc cggcactatc accggggctt ttcctttact atgttcatta aacgaatact
     3481 tttttcactc ctcg
//
LOCUS       DAOMYG010000346         3489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9711_length_3489_cov_8.313628, whole genome shotgun sequence.
ACCESSION   DAOMYG010000346 DAOMYG010000000
VERSION     DAOMYG010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9711_length_3489_cov_8.313628"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PK610_10695"
     CDS             <1..760
                     /locus_tag="PK610_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRJ36448.1"
                     /translation="PSMLPWGQMNVDIVIESTGHFTSMDGAMKHIAAGAKKVIISAPA
                     KTDDVKTVVLGINDHLLDGTEKVISNASCTTNCASPMIKIIHDLCEVESCYVSTVHSY
                     TGDQRLHDAPHPDLRRSRAAALSIIPTTTGAAKAITRIFPELEGKVGGCGIRVPVPDG
                     SLTDITFIVKKPISVNAINKAFHDKSKAELKGILDYTEDPVVSVDCIGNKHSCVFDAQ
                     LTSVIGNMVKIVGWYDNEGGYSARLVDLVKKISA"
     gene            complement(762..1508)
                     /locus_tag="PK610_10700"
     CDS             complement(762..1508)
                     /locus_tag="PK610_10700"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020023298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRJ36449.1"
                     /translation="MKQGKKIYFASDFHLGVPNYTESRKREDRIVKWLNEIEKDAQEI
                     YLVGDVFDFWFEYKRAIPKGFIRLQGKIAELCDKGIPVKVFIGNHDMWMFDYLPTELG
                     IELFREPVTVNYSGKKFMIGHGDGLGPGDHGYKMIKKIFRNRVCQWMFARLHPNFGIG
                     IANYFSKSSRKKTKHEDQYLGDEKEYLVNYCKEILQREEYDYFVFGHRHLPIDMKIGN
                     ARYINLGDWIKFYTYAEFDGTTMTFKTFEG"
     gene            complement(1505..>3489)
                     /locus_tag="PK610_10705"
     CDS             complement(1505..>3489)
                     /locus_tag="PK610_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRJ36450.1"
                     /translation="FGLETVIMHEVGHNWFYGILGSNERDHPWMDEGLNSFNEMRYIE
                     TKYPELTLLASFAPQLSSKNEFFGTKKVKQRSQYYLMYMMNARRNLDQPIEEKSENYT
                     STNYGGIVYSKTALAFDYLRAYLGDKVFDKCMQTYYERWKFKHPSPEDLKDVFQEISQ
                     KDLSWFFDGVIQGNEVIDYKFKRKMRVDRTSTKTYTWMKYEEHLVVVNEGTVAVPFCI
                     NGIKGDTVFNTIWYAGHTGKQPVAFPNGPYDYIKIDARERMPEVDRKNNMLKEKGLFR
                     RIEPVRLQFITSIEDPKKSVLYWTPVAGYNLYDGPMLGLLVYNNLFPTRPLEWQIMPM
                     FGFASKKPIGYGHINQRINTRKSELFNHLDIGLNVSSFSTDDYISGFFTTTTNYYKVA
                     PEIFFQFKKKKARSVNDHSVLLRSVYTIENSEYACAECLPGITPPEKFSDNHLYNELR
                     YTYRNSTTLRPWAGELFVQQHQDFLKVSIDIVNKFIYNVKNDFIETRFFAGKFIYNNS
                     LSARYNFRADGSRGRNDYTYDHVFPGRNAVDGIWANQFVNNHGGLKIGTANGQSVDWM
                     LGGNVRATLPVVPFNLFADAVLSGNPGINPVFLFDAGVYLPLLGKTVNVYFPLIWSGI
                     IKSEYSANGWKYNRAIRFTFDLAGKNPFRAIKNING"
BASE COUNT          985 a          723 c          708 g         1073 t
ORIGIN      
        1 tccttctatg ttaccatggg gacaaatgaa cgttgacatt gtgatcgagt caacgggcca
       61 tttcacttct atggacggcg cgatgaaaca tattgctgca ggagcaaaaa aggtaatcat
      121 ttctgcacct gcaaagacag atgatgtgaa aacagttgtg ttgggtatta acgatcattt
      181 gctcgatggc actgaaaagg taatttcaaa tgcatcctgc accacgaatt gtgcttcacc
      241 gatgatcaag ataattcatg atttatgtga agttgaaagt tgctacgttt ctactgtgca
      301 ttcttatacc ggcgatcagc gcttacacga tgcaccacat cccgatcttc gacgttcaag
      361 ggcagcggca ttatcaataa ttccaaccac taccggcgca gcaaaggcta taacacgtat
      421 ttttcctgag ctcgaaggca aggttggcgg ttgtggaatc cgtgtaccgg ttccggatgg
      481 atcgcttacg gatattactt ttatcgtaaa aaaaccgatc agtgtaaatg ctatcaataa
      541 agcctttcat gataagtcga aggctgaatt gaaagggatt ttagattaca ctgaagaccc
      601 ggtagtttca gtagattgta tcggtaataa acactcctgt gtatttgatg cccaactcac
      661 ttctgttatt ggaaatatgg tgaaaattgt aggatggtat gacaatgaag gaggatattc
      721 ggctcgtttg gtagatctgg tgaaaaagat ctccgcatga tttaaccctc aaaggtttta
      781 aatgtcattg tggttccgtc aaactctgca taggtgtaga atttgatcca atcgccaaga
      841 ttaatatacc tggcattacc gattttcatg tctatgggaa ggtgtcgatg cccgaaaaca
      901 aaataatcat attcttcacg ctgcaaaatt tccttgcaat agtttacaag atattccttt
      961 tcatcgccca ggtattgatc ttcatgtttc gttttctttc tgctgctttt ggagaaataa
     1021 ttggcaattc caattccaaa attaggatgc aatcgcgcaa acatccattg gcaaactcta
     1081 tttcgaaata tcttttttat cattttgtaa ccatggtcac ccgggccgag tccgtctcca
     1141 tgtcctatca tgaatttctt tcctgagtaa ttaacagtaa caggctctct gaatagctca
     1201 attccaagtt cagtgggcaa atagtcaaac atccacatat cgtggttgcc aatgaaaacc
     1261 tttacaggaa ttcccttgtc gcataattct gcgatttttc cttgtaagcg tataaatcct
     1321 tttgggatag ctcttttgta ttcgaaccaa aaatcgaaaa catctccaac gagataaatt
     1381 tcctgagcgt ccttttcaat ttcattgagc catttaacaa tacggtcttc tcgctttctg
     1441 ctttcagtgt agttgggaac accaagatga aagtcgctcg caaaatatat ttttttaccc
     1501 tgcttcagcc gttgatgttt tttatggcac ggaatggatt ttttccggcg agatcaaacg
     1561 taaatcgaat cgcacggttg tatttccatc cgtttgctga atattctgac ttaataattc
     1621 ccgaccagat caatgggaaa tacacattaa ctgtttttcc gagtaaaggt aaatagacac
     1681 cggcatcaaa tagaaaaaca gggttaatcc ctggatttcc gctgagtacc gcatctgcaa
     1741 atagattaaa tggaacaacc ggaagcgttg cacgcacatt tccgcccagc atccaatcca
     1801 cactctgtcc atttgccgtt ccgattttta atccgccatg attattgaca aactggttgg
     1861 cccatattcc atcaactgca tttcgtccgg gaaaaacatg atcgtaggta tagtcattgc
     1921 gaccacgtga accatcggct ctgaagttat atcgcgcact cagcgagttg ttgtaaataa
     1981 atttaccggc aaaaaaacgc gtttcgataa aatcattctt tacgttgtaa atgaatttgt
     2041 ttacaatatc gatcgaaacc ttgaggaaat cctggtgctg ttgcacaaat aattcacctg
     2101 cccaaggacg aagtgtagtt gaattccggt aggtataacg taattcattg taaagatgat
     2161 tgtcactgaa tttttcaggt ggggttatac ccggaagaca ttctgcacag gcgtattccg
     2221 aattttcaat ggtgtaaact gaacgaagta ataccgaatg atcattaaca ctccttgctt
     2281 ttttcttttt gaactggaag aatatctcgg gtgcaacttt gtaatagttc gtagttgtgg
     2341 tgaagaaacc tgagatataa tcatccgtag aaaaagagga aacgttcagc ccaatgtcca
     2401 aatgattgaa gagctcactt tttctggtat tgatcctttg attgatgtga ccgtaaccga
     2461 ttggcttttt ggatgcaaaa ccaaacatgg gcatgatctg ccattcaagt ggacgtgtag
     2521 gaaataagtt gttgtaaaca agcaatccaa gcattgggcc atcgtaaaga ttgtatcccg
     2581 caaccggtgt ccagtacaac acactttttt tcgggtcttc tatactggta ataaactgaa
     2641 gtcgcacggg ttcaattctg cggaatagtc ccttttcttt gagcatgttg tttttcctgt
     2701 caacttctgg cattcgctca cgggcatcta ttttgatata gtcatacggt ccattgggaa
     2761 aagcgaccgg ttgttttccg gtatgtcctg cgtaccaaat ggtattaaaa accgtgtctc
     2821 ccttaatacc gttaatacaa aatggcacag caacagttcc ttcattcacc acaacaagat
     2881 gttcttcgta cttcatccag gtgtatgttt ttgttgaggt gcggtcaacg cgcattttgc
     2941 gtttgaattt gtaatcgata acctcattcc cctgaattac tccatcgaaa aaccaactca
     3001 gatctttttg ggaaatttcc tggaaaacat ccttgagatc ctccggtgag gggtgtttga
     3061 atttccaacg ttcgtaatag gtttgcatgc acttgtcgaa caccttatcc ccaagatagg
     3121 cgcgcaagta atcaaatgct aatgcagttt tggaatacac aattccgcca taattggtag
     3181 aggtgtaatt ctcggatttt tcttcgatgg gttgatcaag attgcggcgg gcattcatca
     3241 tgtacatcaa atagtactgc gaacgctgtt tcactttttt cgtcccgaaa aattcattct
     3301 tagaggaaag ttgaggtgca aaagatgcaa gtaaagtgag ctcagggtat tttgtttcga
     3361 tataccgcat ctcattgaat gagttgagac cttcatccat ccaggggtgg tctctttcat
     3421 tggaaccaag aattccatag aaccaattgt ggccaacttc atgcatgatt actgtttcca
     3481 gtccaaaat
//
LOCUS       DAOMYG010000347         3487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9722_length_3487_cov_7.365967, whole genome shotgun sequence.
ACCESSION   DAOMYG010000347 DAOMYG010000000
VERSION     DAOMYG010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9722_length_3487_cov_7.365967"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="PK610_10710"
     CDS             <1..1019
                     /locus_tag="PK610_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36451.1"
                     /translation="LTGILLIFSGCFSNKKDNPDPAFYYWKTAFNFSEEDVKFCNENN
                     IKELYVRFFDLEYNWEHTSLIPVARLQNKTPFPNHFSVVPVIYIDNESLLQVNKNKIE
                     DVARKTWAKINRMHHRYAKTPLREIQFDCDWNESTKELFFYFLKVFRKYSGTTLISST
                     IRLYQYKYHEKAGVPPVDKGVLMYYNMSDIRDESIDNYIFDHKEGSKYLESADHYPLM
                     LDVALPIYKQGVEYNAHGEIVRLIQHEKMQEMISTGEAQPLDDNRYSVDRSDYYSGFS
                     FFGDAYVEHIIRYEEVSKNDLINAADELALHFPGARVLFFDLQQTYYKNYPKHFFHEL
                     KDRF"
     gene            997..3318
                     /locus_tag="PK610_10715"
     CDS             997..3318
                     /locus_tag="PK610_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36452.1"
                     /translation="MNSRIVFSLIAVIYTLPVISCSWWPMPEQYRFALFQPHDNYHKE
                     MYGIGFTSELWRDRSNIHGIGYDREINLKEWEKESGIKAAFIQEIIYHHSAYALTEPT
                     TTIRKNPFYRWLVKKENKLYFDYLIFAKENENYYEIAIDPWEEDKMEDQSSDLFHLDS
                     LLEHRFMQHPFIGTRFHFIQLRTLFYLGNKSEVVSLYEKIFQNSPATSIIDYWAAYYA
                     GVSYLRMEEKEKGAVLLAKSFLHSADKRIAAMEKITRDDLRQILQNNFSPSDKFGLQC
                     MLLAKDPYLNSGLLRQVLEFDPIHPVAELLTTREINKLEDLLLTTPISGYASFGSDIR
                     NGYNWGEKKEEDQKEEARFINREFEQTLSTINSLCKKDHSVFYDIAQIHLHAVGNRPQ
                     KISRLSAKKIPKELQLQADLSMWFAASRNSVNSEEMMEQLFPLITKMEKDERESYSPG
                     IFERSLAWMYNHAHENRNKALAALILHQIEENYHIIFEKSVSIPLYGAMVHTYLYQEM
                     NADDAKKLADFIDDDRLATKSIQWLCREIKKEKENVYQLKDWCGTVVLRDKKMELALK
                     IWKGIPQRHWKEYPYTEYLDANPFSAHSGNMHKTIKKVNFTKPEIAKKLLLYDSLYTY
                     GSPEEQLNALKRKAAYAYNTTYFGNSWIMNRAWWSDGDEPMMNNEAGKNYYGCYEAKS
                     LYLELLKKSKDPEEKAHALSMLARCHKNEVIWKSYDPRAYYHEREEKDYTENNPWVQQ
                     LERDYSQTAYVRNLFSSCGGREEYWWWFNRIEY"
BASE COUNT         1156 a          720 c          698 g          913 t
ORIGIN      
        1 cattaaccgg tattttactg attttctcag gatgtttttc aaataaaaaa gacaatccgg
       61 atcctgcttt ttattattgg aaaacagcct tcaatttctc cgaggaagat gttaaattct
      121 gtaatgaaaa taacatcaaa gaattgtatg tccgcttttt tgatctggaa tacaactggg
      181 aacataccag tctgattccc gttgcccggc tccagaataa aacgccattt cccaaccatt
      241 tttcggttgt tcctgtgatc tacattgata atgaatcact gttgcaggta aacaaaaaca
      301 aaatagaaga tgtagcacgt aaaacctggg caaaaattaa cagaatgcat caccgctatg
      361 caaaaacacc gcttcgggaa attcaattcg actgcgactg gaacgaaagc acaaaagaac
      421 ttttcttcta ttttcttaag gtattccgga agtactccgg cactactcta atcagttcaa
      481 caattcggct gtatcaatac aaatatcatg aaaaagccgg agtccctcca gtagataaag
      541 gagtgcttat gtattacaac atgagcgata tccgcgatga atcaatcgac aattatattt
      601 tcgatcacaa ggaaggttcc aagtacctgg agtcggcaga ccattatcct ctgatgctgg
      661 atgtagcatt gccgatttac aaacagggag ttgaatacaa tgcacatgga gaaattgtgc
      721 gactgattca acatgaaaaa atgcaggaaa tgatttcaac cggagaagcg cagcctttgg
      781 atgacaatag atattcagta gatcgatccg attactattc aggtttttcc tttttcggag
      841 atgcttatgt agaacatatt atccggtatg aagaagtcag caaaaatgat ttgataaatg
      901 cagctgatga acttgcgctc cattttccgg gtgctcgggt attgtttttt gatctgcagc
      961 aaacctatta taaaaactac cccaaacact ttttccatga actcaaggat cgtttttagt
     1021 ctgatcgcag tcatttacac cctacccgta ataagctgca gttggtggcc tatgcctgag
     1081 caatacagat ttgcattgtt tcagccgcat gataattacc acaaggaaat gtacggcatc
     1141 ggattcacct cagaattatg gcgtgacaga agtaatattc acggaattgg atacgacaga
     1201 gaaatcaatt taaaagaatg ggaaaaggaa agcgggatca aagcagcatt cattcaggaa
     1261 atcatttacc atcattcagc ctatgcgctg acggaaccca caacaacaat tcgaaaaaac
     1321 cccttctacc gatggcttgt aaaaaaagaa aataagctct atttcgacta cctgattttt
     1381 gccaaagaga atgaaaacta ttatgaaata gccatcgatc cttgggaaga agacaagatg
     1441 gaagaccaat caagtgattt atttcatctc gacagcttgt tggagcatcg attcatgcaa
     1501 caccctttta tcggcacaag atttcacttt attcaattac gtacactttt ctaccttgga
     1561 aataaatcag aagttgtatc actttacgag aagatttttc agaattcacc agcaacaagc
     1621 atcatcgatt actgggcagc ttattatgca ggagtaagct atctgaggat ggaagaaaaa
     1681 gaaaaaggcg ctgttttact tgcgaaaagt tttcttcatt cggcagacaa gcgaattgca
     1741 gccatggaaa aaattacccg cgatgatcta aggcaaattc ttcagaacaa tttcagtccc
     1801 tcagataaat tcgggctaca atgcatgcta ctggcaaaag acccttattt gaattccggt
     1861 ttattgcgac aagttctgga attcgacccg atacatcctg tggctgaatt actcaccacc
     1921 cgggaaatta acaaattgga agaccttctg ctgactaccc caatttccgg ttacgcttcg
     1981 tttggttcag atattcggaa cggttacaat tggggcgaaa aaaaagaaga agatcaaaaa
     2041 gaagaagccc ggtttatcaa tcgcgaattc gaacagacgc tgtcaacgat aaattccctt
     2101 tgcaaaaaag atcattcggt attttacgat attgctcaga ttcatcttca cgcagttggt
     2161 aatcgtccac aaaagatttc ccgacttagt gcaaaaaaaa tcccaaaaga acttcagctt
     2221 caggcagacc tcagcatgtg gtttgcagca tcgcgaaatt cggtcaattc agaagagatg
     2281 atggagcaat tgtttccgtt gattaccaaa atggagaagg atgaaaggga atcctattcg
     2341 cccggaattt ttgaacgatc gctggcgtgg atgtataacc atgcacatga gaatagaaac
     2401 aaagcacttg ctgccctcat ccttcatcag atcgaagaaa attatcatat catcttcgaa
     2461 aaatcagttt ccattccact ttacggagcg atggtacaca cctatctcta ccaggaaatg
     2521 aatgctgatg atgccaaaaa acttgcggat tttattgatg atgaccgttt ggcaacgaaa
     2581 tccatccaat ggctttgccg cgaaataaaa aaagaaaaag aaaatgttta ccaactgaaa
     2641 gactggtgcg gaacggttgt gttacgcgat aaaaaaatgg aattagccct gaaaatctgg
     2701 aaaggaatcc cacaaaggca ttggaaggaa tatccataca ccgaatatct tgatgccaat
     2761 cccttttcag cacattcggg aaacatgcat aaaacaatca aaaaagtgaa ttttactaaa
     2821 ccggaaattg caaaaaaact attattgtac gattccctgt acacctacgg atcaccggag
     2881 gaacaattaa acgctttaaa aaggaaggct gcctatgcat ataacaccac ctactttggc
     2941 aattcatgga tcatgaaccg cgcctggtgg tcggacggtg acgaaccgat gatgaacaat
     3001 gaagcaggca agaactatta cggttgttat gaagcgaaat ccttatacct cgaactactg
     3061 aaaaaaagca aagatcctga agaaaaggcc catgcgctga gtatgcttgc ccggtgccac
     3121 aagaacgaag tgatctggaa aagttatgat ccccgggcat attaccatga acgggaagag
     3181 aaggattata cagaaaacaa cccatgggtt cagcaattgg aacgggacta ttcccaaacc
     3241 gcttatgtac gcaacctgtt cagttcctgc ggtggacggg aagaatactg gtggtggttc
     3301 aatcggatcg aatactgaat ttttcccttt atttttcttt ccacaagcag tatacttcac
     3361 cgattcttgc tatttttacc catcgccctc aaaaagcaag ggtgttaact atcaagagtt
     3421 tagaatgcaa tttactgcac aacaaatagc cggcatcctg gaaggaacgg tagaggggaa
     3481 tcctgat
//
LOCUS       DAOMYG010000348         3485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9729_length_3485_cov_10.001458, whole genome shotgun sequence.
ACCESSION   DAOMYG010000348 DAOMYG010000000
VERSION     DAOMYG010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9729_length_3485_cov_10.001458"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PK610_10720"
     CDS             <1..242
                     /locus_tag="PK610_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36453.1"
                     /translation="LHTIDLMDGDTIYIFSDGFADQFGGENGKKMKYSRFKELLLTGH
                     FFTMPEQRQRLNVEFEKWKGDLEQLDDVCVIGVRV"
     gene            complement(433..1449)
                     /locus_tag="PK610_10725"
     CDS             complement(433..1449)
                     /locus_tag="PK610_10725"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ36454.1"
                     /translation="MAKFFTIDIHTHILPEHIPNWKERFGYGGFIHLEHHKPCCARMM
                     KDDKFFREVEDNCWSAETRMKECDHQTVDVQVLSTVPVMFHYWAPAEHCLETSIFLND
                     HIADVVKRYPHRFAGLGTLPMQDTALSIQELKRCREIGLKGIQIGTNINGLNLSEPQF
                     FPLFEACQELDMCVFIHPWDMMGESQIQRYWLPWLVGMPAETSRAICSLIFGGIFERL
                     PNLRVAFAHGGGSFPATLGRIQHGFDVRPDLCAIDNPVPPKNYLKKFWIDSLIHDPKM
                     LDVVVDMFGVEKIAMGTDYPFPLGELEPGKLIRESKYSDTEKEMMLSGSALEWLGMKR
                     EDFE"
     gene            complement(1452..1988)
                     /locus_tag="PK610_10730"
     CDS             complement(1452..1988)
                     /locus_tag="PK610_10730"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HRJ36455.1"
                     /translation="MSLILPFNFKQWIEDNRHLLKPPVNNKVIYTDTEFIVMVVGGPN
                     ARKDYHLNESEEFFYQIEGDMTVKVQVDGKAVEVQIREGDIFLLPPNIPHSPIRPANT
                     IGLVIERKRRGEEKDGLLWFCDKCNHKLYDKRFVMKSIERDFLPTFREFYMSEDLRTC
                     KNCGHVMEVDERFVDKDV"
     gene            2163..2951
                     /locus_tag="PK610_10735"
     CDS             2163..2951
                     /locus_tag="PK610_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ36456.1"
                     /translation="MNLDLSGKKAIVCGSTDGIGKASAIELAQLGASVILVARNEDKL
                     VRTRKELDTSKGQEHHYIVADFDFAPELKMRVEDYISETGPVQILVNNTGGPAGGPII
                     NAKTEEFLHTFNAHLICNHILVQAVVPGMKESGYGRIINIISTSVKQPLPGLGVSNTI
                     RAAVANWSKTLAGELGAYNITVNNVLPGATMTQRLQSIAEKKSAASGKSVEEILAHMA
                     AEVPMARVGQAHEIAAAVAFLASPAASYINGINVPVDGGRTGCL"
     gene            complement(3295..>3485)
                     /locus_tag="PK610_10740"
     CDS             complement(3295..>3485)
                     /locus_tag="PK610_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36457.1"
                     /translation="FTLFICCQCKFFLSGAKSSATLSPTCTKALRKGVPLFFVLNIGN
                     VVKFEISINSNQNKETLR"
BASE COUNT          940 a          800 c          815 g          930 t
ORIGIN      
        1 ctttacacac aatcgattta atggatggag ataccattta tattttttcg gatggttttg
       61 ccgatcagtt tggtggagag aacggaaaaa aaatgaagta ttcacgcttt aaggaattat
      121 tgctcaccgg acatttcttc accatgcccg aacagcgtca acgcctcaat gttgaatttg
      181 aaaaatggaa gggtgacctg gaacagctgg atgatgtttg tgtgatcggt gtgcgggttt
      241 gatttttaac cactaagaca ctaagggcac ttagaaacac taagggttta accgcatcct
      301 ccttcgtttc aatacgacgg gcagtttggt gggcaagctg gttaattggg ggctggttaa
      361 ttagggaatt gggggactgg ttaaaaagga atctctttcc ccatttactg atcaccgatc
      421 actgttcacc attcactcaa aatcttcccg tttcatcccc aaccactcca gtgctgaacc
      481 actcagcatc atttcctttt cggtgtcgct gtatttcgat tcgcggataa gtttgccggg
      541 ttcgagttct ccgaggggga aagggtagtc ggtgcccata gcgatctttt ctactccgaa
      601 catgtctaca acaacgtcga gcatttttgg atcgtgtatg agtgaatcga tccagaattt
      661 tttgaggtaa ttttttggag gaacaggatt gtcgattgcg cataaatccg gacgcacatc
      721 gaaaccgtgt tgtattctgc cgagtgttgc agggaaagat cctcctccgt gtgcaaaggc
      781 aacgcgaaga tttggtaggc gctcaaatat tccgccgaaa atgagtgagc aaatggcgcg
      841 cgatgtttcc gccggcatgc ctaccagcca gggtaaccag tagcgttgga tctgcgattc
      901 gcccatcatg tcccaggggt ggatgaatac acacatgtcg agttcctgac aggcttcaaa
      961 tagggggaag aactgcggct cactcaaatt taatccgttg atgttggtac cgatctgaat
     1021 gccttttaat ccgatctccc tgcagcgttt taattcctga atggaaagtg cggtgtcttg
     1081 cataggaagc gttcctaatc ctgcaaaacg atgcggataa cgtttcacca catcggcaat
     1141 gtgatcgttt agaaaaatgg atgtttccag acagtgttct gcaggtgccc aataatgaaa
     1201 cataacgggt acagtagaaa gcacctgcac atctaccgtt tgatgatcgc attctttcat
     1261 gcgtgtttct gcgctccagc agttgtcttc tacttcacgg aaaaatttat cgtctttcat
     1321 catgcgcgca caacaaggct tgtggtgctc cagatgaatg aacccgccgt aaccgaaacg
     1381 ctctttccaa ttgggaatgt gctccggtaa aatgtgggtg tgaatgtcga tggtaaaaaa
     1441 tttcgccacg gttatacgtc cttatccaca aagcgttcat ccacttccat cacgtgtccg
     1501 caattcttgc aggtgcgaag atcttccgac atgtaaaact cgcggaacgt aggaagaaaa
     1561 tctctttcga tgctcttcat cacaaaacgt ttgtcgtaga gcttgtgatt gcacttatcg
     1621 cagaaccaca gcagaccgtc tttctcttca ccgcggcgct tgcgttcaat gacgagtccg
     1681 atagtattgg ccggacgaat gggtgaatgc gggatattgg gaggaagcaa aaagatatca
     1741 ccttccctga tctgtacttc aacagctttt ccatccactt gaactttcac cgtcatgtca
     1801 ccttcgatct ggtagaagaa ttcttcactc tcgttgaggt gataatcctt gcgggcattc
     1861 ggaccaccca ccaccatcac gatgaattcg gtatcggtgt agattacttt gttattgacc
     1921 ggcggtttga gcagatgacg gttgtcttcg atccactgct tgaaattgaa agggagaata
     1981 agactcatgg ctggaaattg tgctttaaaa atagcaattc taggcataca agaggacctg
     2041 catgaagaaa cacaggcata gctgaccgaa atcatcccgc ataagttcct ttaagtgtgc
     2101 gaaatactat catcttgcgg aaaatcagac ggaagcctta ccttcgaaaa aaatacgacc
     2161 ttatgaacct tgatctcagc ggaaagaaag ccatcgtttg cggaagtact gacggcatcg
     2221 gaaaagcatc ggccattgaa ctggcacaat tgggtgcttc ggtcattctg gttgcacgca
     2281 acgaagacaa gttggtgcgt acccgcaagg aactcgatac atccaaagga caagaacatc
     2341 attacatcgt tgccgatttc gacttcgctc ccgaattaaa aatgcgtgtg gaagattaca
     2401 tcagcgaaac aggacctgta caaattctcg tcaacaacac cggcggaccg gcaggcggac
     2461 ccatcatcaa tgcgaaaacg gaagaatttc tacacacctt caatgcgcat ctcatttgca
     2521 atcatattct ggtgcaggcc gtagttcccg gcatgaagga aagcggatac ggacgcatta
     2581 tcaacatcat ttctacctcg gtaaaacaac ctttacccgg actcggtgtt tccaatacca
     2641 ttcgtgcagc tgttgctaat tggtccaaga cgctcgcggg tgaacttgga gcgtacaaca
     2701 tcacagtgaa caatgtgctt cccggtgcaa caatgacaca acgattgcaa tccatcgccg
     2761 aaaaaaaatc ggcagcaagc ggaaaaagcg tagaagaaat tctcgcacac atggcagcgg
     2821 aagtcccaat ggcacgcgta ggacaagcgc acgaaattgc ggctgcagtt gcattcctgg
     2881 catctcctgc ggcttcctac atcaacggga tcaatgttcc ggtagatggt ggacggacgg
     2941 ggtgtttgta ggggttacga ctggtgattt ttcttaacca cttagacact aagagcacta
     3001 agaatcacta aggttttact gcaaagtttc tttgttttga ttcgcgttaa tgtgtttttc
     3061 gttttatgaa tctacagacg ttctactact tcagattttt tcaaggggag caaggcttcg
     3121 attcccctac gcttccctac gctatcggtc gggacaggct cggtcggggc aggtcgctca
     3181 gcccaaagag acgcctacgg cgttacgcta aggtttacca ctggtgattt ttcttaacca
     3241 cttcctcctt cggcggacaa gaagacacta agtgcactca ggaacactaa ggttttaccg
     3301 caaagtttct ttgttttgat tcgagttaat gctgatttca aattttacta catttccaat
     3361 atttaggaca aaaaacaatg gtacaccttt gcgaagcgct tttgtgcagg ttgggctgag
     3421 agtagctgaa gactttgccc cacttaaaaa gaatttacat tgacaacaga taaacaaagt
     3481 aaata
//
LOCUS       DAOMYG010000349         3482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9734_length_3482_cov_10.500730, whole genome shotgun sequence.
ACCESSION   DAOMYG010000349 DAOMYG010000000
VERSION     DAOMYG010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9734_length_3482_cov_10.500730"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PK610_10745"
     CDS             complement(<1..747)
                     /locus_tag="PK610_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36458.1"
                     /translation="MRTFNLIVTLFLLTLTAAAQDDECMTVSDKKVKKLLEQANNPKT
                     TPTDRHQLLKDALAIDPECAPCLYQLGKRAFDKAYKDGTSYHYAEDYFKKLINACPKY
                     HSDPYYYMGVIAYSKEDYETAITWFKQFTEFKITDDSQMAEDHPEKKKDAKAILPECE
                     FYASFFKNLVAFSPQPVENVCTVGDEYLPMISPDNEFLFFTRRIDRRAMGDMTSRMAE
                     EFSMSQRNDINSPFDAGIKLPAPFNIIGDNY"
     gene            complement(879..>3482)
                     /locus_tag="PK610_10750"
     CDS             complement(879..>3482)
                     /locus_tag="PK610_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRJ36459.1"
                     /translation="LLDAENIVLTIDLYSVIFGSEIDIREVGIKNTDVNVMVLDNGKA
                     NYDIAKSDSTKVAEGVDTSETKFALKVRNYYLENFNLVYDDKPGGMYARIVNLNHNGI
                     GDFTQDLFDFKTTTTADTIDFYMSGIPYMKNTKLDSKLDFSMDMKNSKYTFMPEDYIK
                     LNELDLGFDGFLAMTDVDMDMDINFIARKTDFKNIYSLVPAIYTADYNSIKFGGKTEI
                     KGSMKGKMSEQSMPAMNIDLLVENGSFQYPGLPKSASAIFVKANVNSKGDPTMDDMVI
                     DMPVFKMDLGGNKLDAFFNLRNPMTDPGIKAGLKANFNLSTLREVVPVSNATVMSGFI
                     DSDVAIDGRMSMIDKGEYEKFKAEGSIVAKEIVYNDPEMGYPTNLKIMDFRFAPTHLE
                     LAAFDATVDKTQLMAKGKIDNYLAYALKGDVLKGVFSLNSPEIDLTPFMSSGTETAAT
                     PAATPEASAPAADSYVLEIPRNIDFEMNSSIGKIRYPNDPGAPEIVLDNVNGKITLRE
                     GVMSLNQLKLNTLGASVLMSGDYDSRNLAAPSAAFFFDINNMDIKTASETFPMIAKMA
                     PMASKCTGKFSTKLDFNTKLNTKMEPIYETMNGKGTLATKSIYIEGFEPLNRIAEKLK
                     IDRLKKQTINDVSVSYQFREGRVWVDPYDVTINKYKTTIGGSTGFDGTIDYKADMAIP
                     RSEFGSGANDVLNGLMAKANAKAGTTVELGDMVNVRILITGTVADPKVETDLKQQGQD
                     LKNEIKEQIKEAVKEKVEEKVQEVKEDVKEKASAEAEKIMKDAEAKAAQIRAEAKKAA
                     DKTREEGKNAGDKLIAEAGSNPLKKAAAEKSAQKLQKEADEKASKLEKEADEKAQKVL
                     DEGRAQADKLK"
BASE COUNT          812 a          797 c          702 g         1171 t
ORIGIN      
        1 ataattatcg ccaatgatat taaaaggcgc aggtagtttt attccggcat caaatgggga
       61 attgatatcg ttacgctgcg acatcgagaa ttcttcagcc atcctgctgg tcatgtctcc
      121 cattgctctc ctgtcgatcc tccgggtaaa aaagagaaat tcgttatcgg gggaaatcat
      181 tggcaagtac tcatctccaa ccgtgcaaac attttcaacc ggctgcggag agaaggcaac
      241 cagattttta aaaaagcttg catagaattc gcattcggga agaatggctt tcgcgtcttt
      301 ctttttttca ggatgatctt cggccatttg cgaatcatcc gttattttaa attcggtgaa
      361 ttgcttaaac caggttatag ctgtttcgta atcttctttg gagtaggcga tcacacccat
      421 gtagtagtaa ggatcgctgt ggtatttcgg gcaggcattg atgagtttct taaaataatc
      481 ttcggcgtaa tggtaggaag taccgtcttt atatgcttta tcaaaagcac gtttccccaa
      541 ttggtaaagg cagggagcgc attcaggatc gatagccagt gcatctttga gcaactgatg
      601 cctgtcggtt ggtgtggttt tgggattatt ggcttgttcc agcagttttt taactttttt
      661 atcgcttacc gtcatgcact catcatcttg agcagctgca gttaatgtca ataaaaataa
      721 tgttacgatc aggttgaatg ttctcatgct ttgtcctcct aaaaatgacc tccgaacgtc
      781 cttacgaaat ccggcgttga aggttacttt ctcctcaata caattttcgt tcctaaaaag
      841 aactcccctg aaaccgttac agcttcaggg gagtggaatt attttagttt atcagcctgg
      901 gcacgccctt catcgaggac tttctgtgct ttttcatcgg cttctttctc gagcttgctt
      961 gctttttcat ccgcttcttt ttgcagtttt tgtgcgcttt tttcagctgc agcttttttc
     1021 agcggattgc ttcctgcttc ggcaattaat ttatcgcctg cattttttcc ttcctcacgg
     1081 gtcttgtcgg ctgctttttt cgcctctgcg cggatctgcg cggctttggc ttctgcatct
     1141 ttcatgattt tttcggcctc tgcacttgct ttttccttta catcttcttt tacttcctga
     1201 actttttctt ctactttttc tttcaccgct tccttgatct gctccttgat ttcgttcttc
     1261 agatcttgtc cttgttgttt caggtcggtt tcaactttcg gatcggcaac tgttccggtg
     1321 atcaaaatgc gaacattcac catatcgccc agctctaccg ttgttccggc ttttgcattt
     1381 gcttttgcca tcaatccgtt caatacatcg ttggctcctg atccaaattc agatcgtgga
     1441 attgccatgt cggctttgta atcgatggtt ccgtcaaatc ctgtactacc gccaatggtg
     1501 gttttgtatt tgttgatggt tacatcatag ggatcaaccc acacacgtcc ctcgcggaac
     1561 tgatagctta cacttacatc gttgatcgtc tgttttttca accggtcgat cttcaatttt
     1621 tcggcaatgc gattcaatgg ttcaaatcct tcgatgtaaa tggacttggt ggccaacgtt
     1681 cctttaccgt tcatggtttc gtagatcggt tccattttag tattcagctt ggtattaaaa
     1741 tcaagcttgg tagaaaactt accggtacac ttactcgcca taggtgccat ttttgcaatc
     1801 attgggaaag tttccgaggc cgtcttaatg tccatgttgt tgatgtcgaa gaaaaacgca
     1861 gcagagggcg ctgcaagatt gcgcgaatca taatcgccgc tcatcaacac gcttgcgccg
     1921 agtgtattta atttcagctg atttaaactc atcactcctt cacgcaatgt gatttttcca
     1981 tttacattat ccaacacaat ttccggtgcg ccgggatcgt tggggtaacg tatttttcca
     2041 atcgatgaat tcatttcgaa atcgatgttg cgcggaattt ccagtacgta tgaatctgca
     2101 gcaggagcgc ttgcctctgg agtagctgca ggtgttgctg cggtttccgt tcctgaactc
     2161 ataaatggag ttagatcgat ctccggcgaa ttcaaactga acactccttt caatacgtct
     2221 ccttttaagg cataagccag gtaattatcg attttccctt ttgccatcaa ttgtgttttg
     2281 tcaactgtag catcaaaggc tgccaattca agatgggttg gtgcaaaacg gaaatccatg
     2341 atttttagat tggtgggata tcccatttcg ggatcgttat acacgatttc ttttgcaaca
     2401 atgcttcctt ctgctttgaa tttttcgtac tctcctttgt caatcatcga cattcttccg
     2461 tctatggcaa catcgctgtc aatgaatccg ctcatcaccg tggcattgga aaccggtacc
     2521 acttcgcgca aagtcgacag attgaaattc gctttcaatc ctgctttgat tccgggatcg
     2581 gtcatcggat ttctcaaatt gaaaaatgca tcgagtttgt ttcctcccaa atccatttta
     2641 aacacaggca tatcaatcac catatcatcc atcgtcggat cacccttcga attgacattt
     2701 gcttttacaa aaattgcact tgccgatttg ggcagaccgg gatattggaa acttccgttt
     2761 tctacaagca gatcaatatt catggcaggc atcgattgct cgctcatttt tcctttcatg
     2821 ctacctttga tctcagtttt tcctccgaac ttaatactgt tgtaatcggc agtgtaaatg
     2881 gcaggaacaa gcgagtaaat gtttttgaaa tctgttttac gtgcgatgaa attgatgtcc
     2941 atatccatgt ccacatccgt cattgccaaa aagccgtcga aaccgagatc gagttcgttg
     3001 agcttgatgt agtcttccgg catgaaggtg tatttggaat ttttcatatc catcgaaaaa
     3061 tccagtttcg aatcgagttt ggtgtttttc atgtacggaa ttccgctcat gtaaaaatcg
     3121 atggtatctg cagtagtggt ggtcttgaaa tcaaaaagat cctgtgtgaa atcaccaatt
     3181 ccattgtggt tcaaattaac aatacgcgca tacatcccac cgggtttgtc gtcgtaaacc
     3241 agattgaaat tctcgaggta gtaattcctg actttcaacg caaacttcgt ttctgatgta
     3301 tcaacacctt cggctacctt ggtactgtcc gactttgcaa tatcgtaatt ggctttcccg
     3361 ttgtcgagca ccatcacatt gacatccgta ttttttattc ctacttcacg aatatcaatt
     3421 tccgatccga agatcacact gtacaggtcg atggttaaaa cgatgttctc cgcatccaga
     3481 ag
//
LOCUS       DAOMYG010000350         3482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9735_length_3482_cov_8.021593, whole genome shotgun sequence.
ACCESSION   DAOMYG010000350 DAOMYG010000000
VERSION     DAOMYG010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9735_length_3482_cov_8.021593"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PK610_10755"
     CDS             <1..483
                     /locus_tag="PK610_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36460.1"
                     /translation="YCINWNYVFSNMHKKASPNMKAFLRYRDKYPEKLSYDAGLAFSP
                     MELSERTIEAEQLIANGDFLMRDYVLSELSDVTTYIFFGMDNTPSFEYETNELYEESK
                     TALEFLQKNGGPVVAGVVRDFLHHLEVNSWKRTDEMWELYNNEIIRKKLEDKYPLGKQ
                     "
     gene            complement(484..2622)
                     /locus_tag="PK610_10760"
     CDS             complement(484..2622)
                     /locus_tag="PK610_10760"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HRJ36461.1"
                     /translation="MNKLFLFLLFVVAQSHAQRNLSIQEAIPGSDSYRSVERLKNLKF
                     TAVSGFVSWERKDNPAIMLTDLSGKQMELLLLEDYKKALIQAGQRGPKGLPNPQWKSA
                     VLFDFYLDGKKWEFDVKKKQARPSTDTARLVYHENEEHSSIPTLFAYTKENNIVLHNN
                     GRETQITTDGNSNLIYGHAVHRNEFGIEKGLFFSPKSGFLAFYRMDQSMVSDYPIINW
                     LERPAKNENIKYPFAGDKSHHVKLGIYDIKKGTTIYVNTGGDPEQYLTNVCWSPDEQR
                     IFIALVNRDQNEMNLNEYDAQTGAFIRTLFSEKDDKYIEPLHPMVFLKNDPTKFIWQS
                     NRDGFKHLYLYNISGKMIRQLTLGKWEIKDHNGFDASGKNYFFHANMTSPIDQNFCRV
                     ELASGKVVNLTPRSGFHRCVPDASRTWFIDHFSDASTPGIAYRTEVKSGKSTELFRSP
                     DPIADVNKATLQMFTIPNEEGTPLYCRMFLPPGFDATKKYPTVVYLYNGPHSQLVTNS
                     YMGGADMWYQYMAQCGFIMFTLDGRGTSHRGKAFEQAIHRRLGEVEMKDQLSGVNWLK
                     SNSYVDANRIGVHGWSYGGFMTTSLMTRYAGVFKAGVAGGPVIDWSLYEIMYGERYMD
                     SPLENKSGYEENNLLNHISKLKGRLLVIHGADDDVVVWQHSLKLLHKSVEAGVHIDYY
                     VYPGHKHNVRGPERVHLMEKISNYFIEQLN"
     gene            2724..>3482
                     /locus_tag="PK610_10765"
     CDS             2724..>3482
                     /locus_tag="PK610_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ36462.1"
                     /translation="MRFLFLSLLLLRLVHAQDSLVKTIWFAPDSYELTPTLIADIASL
                     WQNGLHMPDGKRLLVIGHTDNQKDSLSNMRLSEQRARAVADVLRKAGVPSDKIHLGVF
                     GANEPVGNNSTLSGRAINRRVEIKIGPAVSQTRKSNVFSYSLQGTNYNQDTITIGSPQ
                     GIILRFPPGCIQPEVAALIRITLFQGEPLFSEFINQSFSSLSTADRPVDLALFLKLTN
                     PTEVFSTRTAFELLLPASEYYSGMQWFEENDYKRS"
BASE COUNT          992 a          775 c          689 g         1026 t
ORIGIN      
        1 tattgcatca actggaacta tgtgttcagc aacatgcaca aaaaagccag tccaaacatg
       61 aaagcttttt tgaggtatcg tgataagtat cctgaaaaac tgagctacga tgccggtctc
      121 gcatttagtc cgatggaact ttcagaacga acaattgaag cggaacaatt gattgcaaat
      181 ggagattttc taatgagaga ctatgtactt tccgaactaa gtgatgtaac aacctatatc
      241 ttcttcggta tggacaatac tccttcattc gaatacgaaa ccaatgagct ctatgaagag
      301 tcaaaaaccg ctcttgaatt ccttcaaaaa aacgggggcc ctgttgttgc aggtgttgtt
      361 cgcgattttc ttcatcatct tgaagtcaat tcctggaaaa gaacggatga aatgtgggag
      421 ctctacaaca atgagatcat tcgaaaaaag cttgaggata aatacccgct tggaaaacaa
      481 taatcagttc aactgctcaa taaaatagtt actgatcttt tccatcaggt gcacccgctc
      541 aggtccgcga acattatgct tgtgtccggg ataaacataa taatcaatat gtactccggc
      601 ttctactgat ttgtgaagaa gctttaaact atgttgccag acaacaacat catcgtcggc
      661 accgtgtatt accaataatc tgcctttcag tttcgaaata tgattcaata gattattctc
      721 ttcataaccg gacttatttt caagagggga atccatatac cgctcaccat acatgatctc
      781 atacaaactc caatcaatta caggtccacc tgcaacacca gctttgaata cacctgcata
      841 acgggtcata agtgaagtgg tcatgaagcc accataactc catccatgaa ctccaatacg
      901 attggcatca acatacgaat tcgacttaag ccaattcact ccggataact gatccttcat
      961 ctccacttca cctaatcttc ggtgaatcgc ttgttcaaaa gctttccctc ggtgactagt
     1021 tcctcttcca tcgagggtga acatgataaa tccacactgg gccatgtatt ggtaccacat
     1081 atctgcaccg cccatatagg aattggtcac caattgcgaa tgcggtccat tatagaggta
     1141 aacaactgtc ggatattttt ttgtggcatc gaatcccgga ggcagaaaca ttctgcaata
     1201 aagcggggtt ccttcttcat tgggaatggt aaacatctga agtgttgcct tatttacatc
     1261 agcaattgga tccggtgaac ggaacagctc cgtgcttttc cctgatttta cctctgtacg
     1321 gtaagcaatt cccggtgtgg atgcatccga aaaatgatcg ataaaccaag tgcgcgacgc
     1381 atccggaaca caacgatgga atccggatct cggagtaaga tttaccactt ttccacttgc
     1441 cagttctacc cgacaaaaat tctgatcgat aggcgaagtc atattggcat ggaaaaaata
     1501 atttttaccg gaagcatcga aaccattgtg atctttgatt tcccatttgc cgagagtgag
     1561 ttgtcgaatc attttaccgc tgatgttgta caaatacaaa tgcttaaatc catcccgatt
     1621 actttgccag ataaattttg tgggatcatt cttcagaaat accattgggt gaagcggctc
     1681 aatatattta tcgtcctttt cagaaaacaa ggttctgata aatgctccgg tttgcgcatc
     1741 atactcgttc agattcattt cattctgatc tctgtttaca agggcaatga aaattcgctg
     1801 ttcatcaggc gaccagcaaa catttgttaa atactgttcg gggtcgccac ctgtattgac
     1861 ataaatggtt gttccctttt ttatgtcata gattccgagt ttcacgtggt ggcttttatc
     1921 tccggcaaaa ggatatttaa tgttttcatt ttttgccggt cgctccagcc aattaataat
     1981 ggggtaatca ctaaccatgc tttgatccat tcggtaaaaa gccaaaaaac cggatttcgg
     2041 agaaaaaaac aagccctttt caattccaaa ctcattgcgg tgaacggcgt gcccataaat
     2101 gagattggag ttcccgtcgg tagttatctg cgtctcccta ccgttgttgt gcaaaacaat
     2161 attgttctcc tttgtatatg caaacagtgt tggaattgat gagtgctctt cgttttcgtg
     2221 gtaaaccaac cgagcggtat ccgttgatgg tctggcctgt ttctttttta catcaaactc
     2281 ccatttcttc ccgtcgagat aaaaatcaaa aagcacagca cttttccatt gcggatttgg
     2341 tagtcccttt ggtcctcgtt gccctgcttg tatcagcgct tttttatagt cttccaataa
     2401 caataattcc atctgcttgc ctgacaaatc ggtgagcatt attgcaggat tatcttttcg
     2461 ctcccaggaa acaaatccgg aaacagcagt aaactttaaa ttcttcaatc gctcaaccga
     2521 acggtaggaa tcggatccgg gaattgcttc ctgaatggaa aggttacgct gagcatgcga
     2581 ttgtgcaacg acaaacagaa gaaaaagaaa gagcttattc ataaactgat ttttttctgc
     2641 aaaatacgtt tttcgtgtct tttcctgaaa gaaggaagta cgcaataacc ctttatcaat
     2701 gaaaatagtg cgtattttta cctatgcgat ttcttttcct tagccttttg ctgctgcgtc
     2761 tggttcatgc tcaggattca ttggtgaaaa ccatttggtt cgctcccgac agctatgaac
     2821 taacgccaac gctcattgcg gatattgcat cactttggca aaatggattg cacatgccgg
     2881 atggaaagcg attattggtg attggtcata ccgacaatca aaaagattcg ttaagtaata
     2941 tgcggctatc ggaacaacgg gccagagctg ttgctgatgt tctccgaaaa gccggagttc
     3001 cctcagataa aattcacctg ggtgtctttg gggcgaatga accggtgggg aataactcca
     3061 ctttatccgg acgcgcaata aaccgcaggg tagagataaa aattggtccg gccgtttccc
     3121 aaacgcggaa atccaatgtg ttttcctatt ctcttcaggg aacaaactac aaccaggaca
     3181 ccataacaat cggttctccg cagggaataa ttctgcggtt tcctcctggt tgtatccaac
     3241 ctgaagttgc tgcattaatt cgcattaccc tatttcaagg agaacctctt ttttcggaat
     3301 ttattaatca gtcattctct tctctctcca cagcagatcg tccggtcgat ctggcattgt
     3361 ttctgaagtt gacaaaccca actgaagttt tttcaaccag aactgctttt gaactattac
     3421 ttcctgcaag cgaatattat tcaggcatgc aatggttcga ggagaatgat tataaacgca
     3481 gt
//
LOCUS       DAOMYG010000351         3482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9736_length_3482_cov_7.882988, whole genome shotgun sequence.
ACCESSION   DAOMYG010000351 DAOMYG010000000
VERSION     DAOMYG010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9736_length_3482_cov_7.882988"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PK610_10770"
     CDS             complement(<1..268)
                     /locus_tag="PK610_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRJ36463.1"
                     /translation="MKIAAGIDIGGTSTKFGLVDFQGRIIDRGEISTAKYPAPEELVA
                     KVCDAIRELLKRNNFKNEMLGGFGIGSPNGNYFTGRVEFAPNMNW"
     gene            complement(324..1718)
                     /locus_tag="PK610_10775"
     CDS             complement(324..1718)
                     /locus_tag="PK610_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ36464.1"
                     /translation="MNRSFLTLISVFFFWGFVAASNDILIPVFKDELDLEQWQSQLIS
                     FAFYISYTVGSVLYQLYAVLFGRDLLSRIGYKNGIALGLVISASGTLLFIPAANTASY
                     PLMLSGLFIVGLGFSLQQTAANPLAIRLGDPAKSSQRLSLAGGINNVGTTIGPVLLGL
                     AIFGNVSTGNTIANIESIKIPYLILGAAFLLVALAIKYSKIPDQLEDSLEVDTNEQTN
                     NHRSPLSYPQLWLGMIAIFVYVGVEVSTASNLPEYLKSESGIDTNATAPYISLFWASL
                     MIGRWTSAAGVFNVQGGLKTALGFLMPYLAFGLFILVNVLVSNDVSSFYYYPFVILVM
                     IAGDLLSKGNPSKQLFIFSVLGILALVVGMMTSGLVSIYAFISVGLFCSTLWPCIFTL
                     AITGLGGATNRGSGFLIMMIMGGGLISLLQGWLANENLFGIRLSYLTGVFCFIFLAWY
                     AYRFRNLVAQSVAK"
     gene            complement(1721..2287)
                     /locus_tag="PK610_10780"
     CDS             complement(1721..2287)
                     /locus_tag="PK610_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36465.1"
                     /translation="MNRILQLLIASLCLVLAFSWLVKPGYSLLMGYNNRAASWDIRKG
                     AFLKTDAIPDSVLVTRNEMNPGFNADEPTYYMDYELYYSFYTRDSVLASGKDNFYNSS
                     NEPLPLLDKLSDWQNVKVYYDTLNPTENYSEETFLLFTTKENQSTQRSFRIALLVAGL
                     IISLLCLIWIIRLIRILSKEPGNSSSNS"
     gene            2346..3314
                     /locus_tag="PK610_10785"
     CDS             2346..3314
                     /locus_tag="PK610_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015024466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ36466.1"
                     /translation="MKRVLITGAAGFLGSHLCDRFIKEGYHVIGMDNLITGDLRNIEH
                     LFKLEQFEFYHHDVSRFVHVPGELDYILHFASPASPIDYLKIPIQTLKVSSLGTHNLL
                     GLAKAKKARILVASTSEVYGDPLIHPQTEEYWGNVNPVGPRGVYDEAKRFMEAMTMAY
                     HTYHNVETRIIRIFNTYGPRMRLNDGRVLPAFIGQALRGEDLTMFGDGSQTRSFCYVD
                     DLVEGIYRLLLSDYVQPMNIGNPDEITIREFGEEILKLTGANQKLISLPLPKDDPKQR
                     QPDITKAKSILGWEPKVKRAEGLRITYEYFKSLPKEELFKKEHRFD"
BASE COUNT         1123 a          793 c          636 g          930 t
ORIGIN      
        1 tccaattcat attgggagca aattcaacac gccctgtaaa ataatttcca ttcggcgagc
       61 ctattccaaa gccacccagc atttcatttt taaaattatt ccttttgagc aattcacgaa
      121 tcgcatcgca aaccttagca acaagttcct ccggcgcagg atactttgcg gtagaaatct
      181 caccacgatc aataattctt ccctgaaaat caaccaaacc aaatttggtg cttgtccccc
      241 caatatcaat acctgcggca attttcatta aaacgattta gaattgatta tcctaaagat
      301 aattaaattc tttatcgtga ttgctatttc gcaacagatt gagcaaccag gttcctgaac
      361 cgataggcat accaggcaag gaaaatgaag cagaatacac ctgtgaggta acttagtcga
      421 atcccaaaca ggttttcatt tgcaagccat ccttgtagca acgaaataag ccctcccccc
      481 atgatcatca tgatcagaaa gcctgaacca cgatttgttg ccccacctaa gccggtaatt
      541 gcaagagtaa aaatacaagg ccacaacgtt gagcaaaaca aacccacact gatgaatgca
      601 taaatactta ccaaaccgct cgtcatcatt ccaaccacca aagccagaat tccgagcact
      661 gagaaaataa ataactgttt ggaaggattg cctttactta acaaatcacc tgcaatcatt
      721 acaaggataa caaacggata ataataaaat gatgagacat cattacttac caaaacatta
      781 acaagaataa acaaaccaaa ggcaaggtag ggcattagaa agccaagagc cgttttcaat
      841 cctccctgaa cattaaaaac acccgcagcc gaggtccatc gtcctatcat caaacttgcc
      901 cagaagagag aaatatacgg cgcagttgcg tttgtatcga ttccactttc agattttaga
      961 tactccggta aattacttgc agtagacact tcaacaccaa catacacaaa aatagctatc
     1021 attcccaacc acaattgcgg atagcttagc ggagaacgat gattattggt ttgttcattc
     1081 gtgtcgactt caagtgaatc ctccaattga tccggaatct tcgaatattt aattgcaagc
     1141 gccaccaata ggaaagcagc ccccaatata agataaggga tttttatgct ttcaatgttt
     1201 gcaatagtat ttcccgttga aacatttcca aagattgcga gtcccaaaag caccgggcca
     1261 atggtggttc ctacattatt tatcccacct gcaagactaa gtcgctgcga acttttagcg
     1321 gggtcaccta atcgaattgc aagtggattt gctgctgttt gctgcaatga aaaaccaagt
     1381 cctacaataa acaatccact cagcattaaa ggataacttg cagtattggc tgcaggaata
     1441 aacaataagg ttccggaagc agaaataact aatccgagag caataccgtt cttataccca
     1501 attctgctta gcagatcccg tccaaacaac actgcataca actgatagag cacagatcct
     1561 accgtgtagg aaatataaaa cgcaaatgaa atcaattgcg attgccattg ttcaagatcc
     1621 aattcatcct tgaataccgg aataagtata tcattggagg cagcaacaaa gccccagaaa
     1681 aaaaacacag aaatcagtgt aagaaaagac ctgttcattt ttaagaatta gatgatgaat
     1741 tgccgggttc tttggagagt attcggatca atcggataat ccagattaaa cacaaaagag
     1801 aaataatcaa tcctgcaacc agcaaggcaa ttcggaatga tcgctgtgtg gactgattct
     1861 cttttgttgt gaaaagcaaa aatgtttcct ctgaataatt ctcagtcgga ttcaatgtat
     1921 cgtaatacac ttttacattt tgccagtcgg agagtttatc caacaatggc aatggttcat
     1981 tcgaagaatt gtaaaaatta tcttttcccg atgccaaaac actatccctg gtataaaacg
     2041 aataataaag ctcataatcc atgtaatagg ttggttcatc tgcattgaag ccaggattca
     2101 tttcgttccg ggttaccagc acactgtcag gaattgcatc ggttttaaga aatgctcctt
     2161 tgcgaatatc ccatgaagcc gctcggttgt tgtatcccat gagcaggcta tatcccggct
     2221 taaccaacca gctaaaagcc agtaccagac aaagcgaggc aataagcaac tgtaggattc
     2281 gattcatttt attcaaggga attgaacaaa cttatttatt tttagcgcac aatcatcatt
     2341 tcaacatgaa aagagttctt attaccggag cggccggatt tcttggatca catttgtgcg
     2401 accgttttat caaggaaggt taccatgtta tcggtatgga caatctcatt accggcgatc
     2461 ttcgcaatat tgagcatttg ttcaagcttg agcagttcga attttaccat catgatgtct
     2521 ccagatttgt gcatgttccg ggtgaattgg attatatttt gcactttgcc tctcccgcat
     2581 cacctatcga ttacctcaaa atccctatac aaaccttaaa ggtttcctct ttgggcacgc
     2641 ataatcttct tggacttgct aaagccaaaa aagcgcgcat tctggttgct tctacctccg
     2701 aagtatacgg cgatccgctg attcaccctc aaaccgaaga atattgggga aatgttaatc
     2761 cggttggtcc cagaggtgtt tatgatgaag caaaacgctt catggaagca atgacaatgg
     2821 cctaccacac ctaccacaat gttgaaaccc gaatcatccg catcttcaat acttacggac
     2881 cacgtatgcg attgaatgat ggccgggttc ttcctgcttt tattgggcaa gctcttagag
     2941 gagaagacct caccatgttt ggtgacggat cgcaaacccg atcattttgt tacgtcgatg
     3001 atctggtgga gggcatttac cgtttattgc ttagcgatta cgttcaaccc atgaacattg
     3061 gaaatcctga cgaaataacg attcgcgaat ttggcgaaga gatcctgaag cttaccggtg
     3121 ctaatcaaaa gttgatttca cttcctttac ccaaagacga ccccaaacaa cgtcagcccg
     3181 atattacaaa agctaaatcc atactaggct gggaacccaa agtaaagcgt gcagaaggtc
     3241 tgcgaataac ctacgaatac ttcaaatcac tgccaaaaga agaattgttc aaaaaagaac
     3301 atcgtttcga ctgaacacct cttacttacg gctgttcaat attgaaaaca gaacgtgaaa
     3361 accatttttc tatttctcag tcttttttgc atcggttcaa tttccgcagc aggacaatca
     3421 ttaccgaaaa catcggcaca cctttacagc gatcttcaaa aattaaaaac tgttgggtcg
     3481 gt
//
LOCUS       DAOMYG010000352         3475 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9758_length_3475_cov_8.246199, whole genome shotgun sequence.
ACCESSION   DAOMYG010000352 DAOMYG010000000
VERSION     DAOMYG010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9758_length_3475_cov_8.246199"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PK610_10790"
     CDS             <1..798
                     /locus_tag="PK610_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36467.1"
                     /translation="ALVVFLALRAALHLSSVPKFDLRKYTRKRFIIIGKKDEADRVAG
                     LLKQTNNYADFIAYVHPESGHHESGFSGDISQLNEVVDVMKIDEVIFCARDNSAQQII
                     DRMSRFDHQKIDFKIAQPESLYLIGSNSIDTSGDLYVLDINSISRNVNKRNKRIFDIL
                     CSLILIITTPLVALVNLAHLKVFPSFFRVLFGRLSMVGYSISNPAQYRLPRIRNGVLS
                     TTSNLNTFSPNEETQRKIDIIYAKDYRVSTDIKLVWASLLKMGRTKP"
     gene            898..2217
                     /locus_tag="PK610_10795"
     CDS             898..2217
                     /locus_tag="PK610_10795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRJ36468.1"
                     /translation="MAQVEVLLPKMGESVAEATIIKWLKNEGDTVELDEPIIEIATDK
                     VDSEVPSPNAGILIKRLCNEGDVVQVGKPIAIIGSEAGAVAAPVAPKVEVASNGNGQH
                     HNAPVAAAVGTAVSAPLGKGNGNKFFSPLVRTIAQTEGVTIEELETIQGSGAQGRVTK
                     KDILNYLPNKGKTAAPTQVTAPVQQNATRVEVKEEKRPAIPVGPNDEIIEMDRMRKLI
                     AQYMVDSKRISPHVTSFVEADVTNLVLWREKAKKEFERREKEKLTFTPLLMEAVIKAI
                     KDFPMINISVDGDRIIKKKDINIGMAAALPSGNLIVPVIKNADRLNLVGLTQSVNDLA
                     NRARTNQLKPDEISGGTYTISNVGNFGNVMGTPIIQQPQVAILALGAIRKKPAVIETP
                     YGDTIGIRHMMFLSHSYDHRVVDGSLGGMFVRRVADYLEQFDINREI"
     gene            2236..>3475
                     /locus_tag="PK610_10800"
     CDS             2236..>3475
                     /locus_tag="PK610_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36469.1"
                     /translation="MKLKHAFLFLLFLLGGFAPLFGQGTPPTDEELRKIPIPDRYDIA
                     TELMREKQYYQALRIWKTILEEKPDNANYNFKAGVCYLNMNQDKTMALPHLEKAATKT
                     SKNYNPFDFMEDKCPVDVYYYLGQAYHLNYQFDKAIESFNKFKEQASKKHIFMEDVDR
                     HLSWANNAKSEIASPRQVNTQTVNLGPKINSSYNDYSPVLSLDENALYFTSRRLRTDS
                     SNIRAIIPDDGKYYEDIYVSFRNRETGEWGQAVIVDLFNKVRENEATISVSADGLRIY
                     IYHDVAGDGNIYFSDFVDTAFTEMEPIGDHVNSTSWETHITFTPDGNTAFFVSDRPGG
                     MGGRDIYRVTRLPNGKWSEPFNLGAPINTKYDEDAPFISADGRTIYFSSNSEKSMGGF
                     DVFVSELNDDKLFTEPVPIGV"
BASE COUNT         1044 a          769 c          729 g          933 t
ORIGIN      
        1 gcattggtcg tttttctggc tttacgtgca gctcttcacc tatcgtcggt acccaaattt
       61 gatttgcgca aatacaccag aaaaagattt attatcattg gtaaaaagga cgaagcagat
      121 cgcgttgcag gcttgctgaa acagaccaat aactacgctg attttattgc ctacgttcac
      181 ccagaatccg gacatcatga aagcggattt tcgggcgaca tttcgcagtt gaatgaggtg
      241 gtggatgtca tgaaaatcga tgaggtgata ttttgcgcac gcgacaacag tgcacagcag
      301 atcatcgacc gaatgtcgcg tttcgatcat caaaaaattg acttcaaaat tgcccaacct
      361 gaaagccttt acctcattgg cagcaacagt atcgatacat ccggtgactt atatgtgctg
      421 gatattaatt caatcagcag aaatgtaaac aaacgcaaca aacgcatctt tgatattcta
      481 tgtagcctga ttttaataat aactactccg ttagtagcgt tagtcaacct cgctcatttg
      541 aaggtttttc cttccttttt cagggttttg tttggacgac tatccatggt gggatactct
      601 atttcgaatc cagcccaata ccgattgcca aggatcagga atggggtgct cagcacaaca
      661 tccaatttaa acaccttctc tccgaacgag gaaacacagc ggaaaattga catcatttac
      721 gcgaaagact atcgcgtaag cactgatata aaacttgttt gggcctcatt gttgaaaatg
      781 ggccgtacaa agccttgaaa atctgtcctt tgccaaatac aaacaagtct tgtcggtaag
      841 cagaattcga ttatttttgt agcttattga aaaataatcc atcacattca cagacaaatg
      901 gcgcaggttg aagttttgtt gcccaaaatg ggagaaagtg ttgcagaggc aacgatcatt
      961 aaatggttga aaaatgaagg ggacaccgtt gaattggatg agcccatcat tgagattgca
     1021 accgacaaag tagattctga agttccatct cccaatgcag ggattctgat caagcgtctt
     1081 tgcaatgagg gcgatgtagt tcaggttgga aaaccaattg ccattatcgg ctcagaagca
     1141 ggtgctgtag cagctccggt tgctcctaaa gttgaggttg ccagcaatgg aaacggacaa
     1201 catcacaatg ctcctgttgc agctgcagtt ggtacagctg tttctgctcc gcttggaaaa
     1261 ggcaatggaa ataaattctt ctctccattg gtgcgtacca ttgcacaaac agaaggtgtt
     1321 accattgaag aactggaaac catccaggga tcaggtgcac aaggacgagt taccaaaaaa
     1381 gatattctca attacctccc gaataaagga aaaacagctg ctcccacaca agttactgct
     1441 cctgttcagc aaaacgctac ccgcgttgag gtaaaagaag aaaagcgtcc tgctattccg
     1501 gttggaccga acgacgagat catcgaaatg gatcgtatgc gcaagctgat tgcacaatac
     1561 atggtcgatt caaaacgcat ctctccacat gtcacctcat ttgttgaggc agatgttacc
     1621 aatctcgtac tctggagaga aaaagccaag aaagaatttg aacgtcgcga aaaggagaag
     1681 cttacgttta ccccgctgct gatggaagct gtgatcaagg cgatcaaaga tttcccaatg
     1741 ataaacatca gtgtcgacgg cgaccgtatc atcaagaaaa aagacattaa catcggaatg
     1801 gctgctgctc ttccgagtgg aaatctgatt gttccggtaa ttaaaaatgc cgatcgttta
     1861 aacctcgttg gtcttacaca atcagtaaat gaccttgcca atcgcgcacg aaccaatcaa
     1921 ttaaaacctg atgagatcag tggcggtacc tacaccattt cgaatgtagg taacttcgga
     1981 aacgtaatgg gaacaccgat tattcaacag ccacaggttg ccattctcgc attgggagca
     2041 atacgcaaaa aacctgcagt aatcgaaaca ccttatggtg atactattgg aatccgacat
     2101 atgatgttcc tctcccactc ttacgatcac cgtgttgttg atggttcatt gggaggcatg
     2161 ttcgttcgcc gtgttgccga ttaccttgaa caatttgata tcaatcgcga gatctaaacc
     2221 tcacaccttc ccctgatgaa actaaaacac gcatttctat ttctcctttt cctcctagga
     2281 ggttttgcac ctctgttcgg acaaggaact cctcctaccg atgaagaatt gcggaaaatt
     2341 cccattcccg atcgttacga cattgcaacg gagttgatgc gtgaaaagca gtattatcag
     2401 gctcttcgaa tttggaaaac cattctcgag gagaaaccag ataatgccaa ttacaatttc
     2461 aaagcaggtg tgtgttacct gaatatgaat caggataaaa caatggctct tcctcatctg
     2521 gaaaaggcag ccactaaaac atccaagaat tacaaccctt tcgatttcat ggaagataaa
     2581 tgtccagtag atgtgtacta ctacctcgga caagcttacc atttgaatta tcagttcgat
     2641 aaagctattg agagttttaa taagttcaaa gaacaggctt ccaaaaagca catctttatg
     2701 gaagatgtcg atcgtcatct ctcttgggca aacaacgcaa aatctgaaat cgcttctcca
     2761 cgtcaggtga atacacagac tgtgaacctt ggaccaaaaa tcaactcatc ttacaacgat
     2821 tattctccgg ttctttcact cgatgagaac gcgctttatt ttacctcacg ccgtcttcgc
     2881 accgatagtt ccaatatccg cgctatcatt cctgatgatg gtaaatacta tgaagatatt
     2941 tacgtttcct tccgtaaccg cgaaaccggc gaatggggtc aggcagtaat tgttgatcta
     3001 tttaataaag taagggaaaa cgaagccacc atcagtgttt ctgcggatgg attgcgcatt
     3061 tacatttacc acgacgtagc gggtgacgga aatatttatt tttccgattt tgtggataca
     3121 gcgtttacag aaatggaacc aattggcgac catgtcaatt ccacctcatg ggaaacccac
     3181 attaccttta cacccgatgg aaacactgca ttctttgtaa gtgatcgtcc gggcggaatg
     3241 ggtggtcgtg atatataccg cgtaacacgt cttccaaatg gaaaatggag tgaacccttt
     3301 aatcttggtg cgcctatcaa taccaaatac gacgaagatg ctccatttat ttctgcagat
     3361 ggaagaacga tctatttctc ttccaactca gaaaaaagta tggggggatt cgatgttttt
     3421 gtatctgaat tgaatgatga caagctcttt acagaacccg ttcccattgg tgttc
//
LOCUS       DAOMYG010000353         3457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9812_length_3457_cov_6.036449, whole genome shotgun sequence.
ACCESSION   DAOMYG010000353 DAOMYG010000000
VERSION     DAOMYG010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9812_length_3457_cov_6.036449"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /locus_tag="PK610_10805"
     CDS             complement(<1..1521)
                     /locus_tag="PK610_10805"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ36470.1"
                     /translation="MMIIRVILSVVLVFVFSCAVKAQDADSIRKRINEGNSDTLGVDI
                     ATKHARKLIKNRKLEEGNEYIEQILKEAKRLHYENGIIQAQSVRASYYADKNEFERSD
                     SIYHQVLAFANKRGNKYAVMNTLNSMGLNYFNHGDFELALEHHQQAAQIAKELNDLSA
                     TARYMSNMARIYNLFSDYENAIRYGKRAIELEKREGGDQLSIVIRMINLSNYFIESGR
                     YVEAIKTCRDGVTLNHEIQGESVWDAYLRNNLGLAMMKSGELQQSIKEFEFARRVFSE
                     IADSATLATIASNLAQAYSGLKNWNRASFYLQESKRLAADDALDPESLRYRFEVGALV
                     HEGSGDYRQALSDYRQSILIKDSIYAATQQENIMSLREAFDADMRDDSIRHHKQQAAT
                     ERELRLLDQRISKHKSVWLIVSGLMIAALAFTSLLAFRNYRKKQIAHELLLEQKEIIE
                     EKNKENELLLGEIHHRVKNNLQVISSLLSLQGKSITDENARTAVEAGKLRVRSMELV"
     gene            2104..3237
                     /locus_tag="PK610_10810"
     CDS             2104..3237
                     /locus_tag="PK610_10810"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRJ36471.1"
                     /translation="MTFAQFELNEQLLSTLQKAAFSVPTPIQEQSIPHILAGRDIFGC
                     AQTGTGKTAAFALPVIQHLTQKSITGNQRKIRTLVLAPTRELALQISEQFKLFSAGQN
                     LFQSVIFGGVSQHKQTSELRRGVDIVIATPGRLMDLMNQGFVDLRNIEHLILDEADRM
                     LDMGFINDIRKIISKLPVKRQTLFFSATISKEIAKLAEGMLKNPVQVSVNPVSSTAVK
                     VEQRVYHVRKENKRSLLKHVLNNEQVESAIVFTRTKRGADRVAKDLNNIGIKAEAIHG
                     NKSQNAREKALSGFKSKRFNILVATDIASRGIDVSQLSHVINYEIPEQAETYVHRIGR
                     TGRAGHSGTALSFCDENEAGYLKDINKLIRKPIEVVNNHPFLN"
     gene            3291..>3457
                     /locus_tag="PK610_10815"
     CDS             3291..>3457
                     /locus_tag="PK610_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRJ36472.1"
                     /translation="MKTGTVKFFDETKGFGFISVENGADIFVHSSGLQQNIRENDKVT
                     FETEQGKKGINA"
BASE COUNT         1013 a          770 c          639 g         1035 t
ORIGIN      
        1 cacaagttcc attgaacgaa cccgcaattt cccggcctca acggcagtcc ttgcattttc
       61 atcggtgatg ctttttccct gaagacttaa cagggaggaa atgacctgga gattgttttt
      121 aacccggtgg tggatttcgc cgagtaatag ttcgttttct ttattctttt cttcaataat
      181 ttccttttgt tcgagcaaca gttcatgcgc aatttgtttt ttgcggtagt tccgaaatgc
      241 aagcaaactg gtaaatgcca atgcagcaat cattaatccg gaaacgatga gccatacact
      301 tttatgcttg gaaatacgct gatcgagcaa acgcaattcc ctttcggttg cagcttgttg
      361 cttgtggtga cgaatagaat catcgcgcat atctgcatca aaagcttcac gcagactcat
      421 gatattttcc tgttgcgttg cagcgtatat actatcctta attagaattg attgtcggta
      481 atcagaaagc gcctgtcggt aatcaccgga accttcatga accaaggcgc ccacctcaaa
      541 gcgataacgt aaactttccg gatccaaggc atcatctgca gccaatcgtt tgctctcttg
      601 taaataaaat gatgcgcgat tccagttttt caatcccgaa tacgcctgag caagatttga
      661 agctattgtt gccagtgttg cactatccgc aatttcggaa aacacccttc gtgcaaattc
      721 aaactctttt atgctttgct gcaattcacc ggatttcatc attgctaatc ccaggttatt
      781 gcgcaggtag gcgtcccaaa cagattcccc ctgaatttca tgatttaggg tcacaccatc
      841 tctacatgtt ttgattgcct caacatatct tcccgattcg ataaaataat tactcagatt
      901 gatcatgcgt ataacaatgg acagctgatc tcctccttcg cgtttttcca actcgattgc
      961 ccgctttccg taacgaatgg cattttcgta atcgctaaat aaattataga tccgggccat
     1021 gttgctcata taccgtgcag tggccgaaag atcattcagc tcttttgcaa tctgtgctgc
     1081 ctgttggtgg tgttcaagtg ccaattcaaa atcgccgtga ttgaaataat ttaatcccat
     1141 cgaattaagt gtattcatca ctgcatattt attacctctc ttatttgcaa atgctaaaac
     1201 ctgatgataa attgaatcgg atcgctcaaa ttcattttta tccgcataat aggatgcacg
     1261 aacactctgc gcctggatga ttccattttc gtagtgcagt cgttttgctt cttttagaat
     1321 ctgttcaata tattcattcc cttcttctag ctttcgattc ttaatcagct tacgtgcatg
     1381 tttggtggca atgtcgacgc caagtgtatc cgaattccct tcattaattc tttttctaat
     1441 gctgtcagca tcctgtgcct tcacagcaca gctgaaaaca aaaaccaaaa caactgaaag
     1501 aattacccga atgatcatca ttgaaaatta cagaaagcga ttaaggcgca caaatcaaaa
     1561 aaatgacttc tggccacttc ccgaattatt taccacgttg ttattacaga aaatccattt
     1621 actgagcgag actttagaca tggttccctc gatttaggag agaaccattt ccgtttgaat
     1681 attcattctg cttaattaca tttaagtaat aaagcctcaa tcgtaggaat cattttgaca
     1741 atgtttcttc tgttctttca aatctataaa agcgaatcca ttccctattc ttaaccagat
     1801 cgtctaaacc gatctatttt gtggatgggg gacttttttt tatgaatgac ccggaaatcc
     1861 ataaaacatt aattacctga gacatagaaa aacaaataac tgggaatcca atgattgcat
     1921 tgcgacagtt tgcaccgttt caaaattctt cttatatttg caactcttat tagaagactg
     1981 tactttttct gccggtttgc gcggcttatg cattcgggta tttctcttac aaattttctg
     2041 attaaggact cgcggttgtt cgttgaaaaa cggacctcca agtatttatt tgatacccaa
     2101 ttaatgacat ttgcccaatt tgaactgaac gaacaacttc tctcaacatt acagaaagca
     2161 gcattctccg ttccaactcc tattcaggaa caatctattc ctcacatttt agccggacgc
     2221 gacattttcg gatgtgcgca aaccggaacc ggtaaaacag ctgcatttgc actgccggtt
     2281 atccaacacc ttacccaaaa atcaattaca ggaaatcagc gtaaaataag aacactcgtt
     2341 cttgctccaa cgcgtgaact tgctttgcaa atcagtgaac aattcaaatt gttttctgcg
     2401 ggacaaaatc tttttcagtc ggtaattttt ggtggtgtat cacaacacaa acaaacctca
     2461 gaacttcgtc gcggtgtcga tattgttatt gcaacgcccg gtcgtttaat ggatctgatg
     2521 aatcagggat ttgtagatct tcgcaacatt gaacatctca tcctcgatga agccgaccgc
     2581 atgctcgaca tgggcttcat taatgacatc cgtaaaatca tttccaaact tccggttaag
     2641 cgtcaaactc ttttcttctc tgcaaccatt tcgaaagaga tcgcgaagct tgctgaagga
     2701 atgctaaaaa atccggtaca ggtttctgtt aatccggttt catcaactgc agtaaaagtt
     2761 gaacaacgcg tataccatgt tcgcaaggaa aacaagcgct cacttttaaa gcatgtgttg
     2821 aacaacgagc aggttgaaag tgctattgta tttacccgta ccaagcgtgg tgccgacaga
     2881 gttgccaaag acctcaacaa tatcggtatt aaggcagaag ctatccatgg gaataaatcg
     2941 caaaatgccc gcgaaaaagc actaagcgga ttcaagagca agcgttttaa cattcttgtg
     3001 gcaacagata tcgcttcacg tggtattgat gtgagtcagt tatcacatgt tatcaattac
     3061 gaaattccgg aacaagccga aacatatgtt caccgtattg gcagaacagg tcgtgccggg
     3121 cattcaggaa ctgcgctttc attttgcgat gagaatgaag cggggtattt aaaagacatc
     3181 aacaagctca tccgcaaacc catcgaagtg gtgaacaatc accccttcct taactaagta
     3241 aattctttaa tcaataaaaa gcgctgtaca aggcgcaaaa attaaatcca atgaaaacag
     3301 gtacagttaa atttttcgat gagaccaaag gtttcggttt tatcagcgtt gaaaacggag
     3361 cggacatttt tgttcactct tcaggacttc aacaaaacat tcgtgaaaac gacaaagtaa
     3421 cttttgaaac cgaacaaggt aaaaaaggta tcaatgc
//
LOCUS       DAOMYG010000354         3432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9921_length_3432_cov_8.296713, whole genome shotgun sequence.
ACCESSION   DAOMYG010000354 DAOMYG010000000
VERSION     DAOMYG010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9921_length_3432_cov_8.296713"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PK610_10820"
     CDS             complement(<1..793)
                     /locus_tag="PK610_10820"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ36473.1"
                     /translation="MKKLLLFSGFLSLNALAIAQCGQRYHDKIFTDSTVSNIQYGSNV
                     NRLNQAENLNLDIHFPKGDTEAERPLIIIAHGGNFLGGSKTGSDVLPMAKDLARMGYV
                     VSSIDYRVGMTNFPFPGPDDGDATESVMRAVHDARASVRFFRKSYENGNPYRIDTTKI
                     FFAGVSAGGFMALHLAYLDQMSEYPPQADTVNQYGLNGGLVGLSGNPGYSSKVHAIVN
                     IAGAIGDTAWIKAGDEPVLNFHGTADATVPYGSDIIQLLGVYPLLM"
     gene            complement(804..2030)
                     /locus_tag="PK610_10825"
     CDS             complement(804..2030)
                     /locus_tag="PK610_10825"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HRJ36474.1"
                     /translation="MKRSLLALFGMLPLFVQAGGFQLNLQGQKQTGMGHTGTGLLSDA
                     STVLFNPGGISLLDSSLNINAGLNLLFPRVRYLAPFPGNYTTETVHNTGTPFHFYVAS
                     RLEGSKRLSFGFGIYTPFGSKQQWEDDWVGQFLIREIDLKTICFQPSFSFKINDELGI
                     GAGFILATGTFGLRKGIPAQDSTGAYGEARLNGRASGFGYNAGVFYKGKNGFSAGIDF
                     RSSILAKIIDGSAEFSVPPALNSYFGTTTFNTSIRLPFTASLGLGYTKDKHRVALDVN
                     YVGWSSYDSLVIDFKDNSEKLADNRSPRNYKNSFIFRAGYEHRLNDQLQIRGGAYFDM
                     TPVQDGYLTPETPDVNKIGITAGASVRLKKGFGIDASFLYIEGMKRTDTNLETGFGGT
                     YKSRAIIPGISLSFVF"
     gene            complement(2112..2750)
                     /locus_tag="PK610_10830"
     CDS             complement(2112..2750)
                     /locus_tag="PK610_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ36475.1"
                     /translation="MKTQIFLLIFVAALATESSAQRPTNQAGEQTIYNGTPNGKSKPK
                     ANPGQKAGASDKVMADPKKTSIENSGGMNGQNRVAQTDKQMADPKATSIEVADGNEQR
                     APHADAQEADSKAIAIETDMSQPTRTETVNASIDFVVYPNPATDLLIVQSTNDQMYTI
                     QLLDLSGKSVRSSSHQSGSGSIRMDVSDLPRGVYFVSLENTENRLTKKVLLT"
     gene            3068..3277
                     /locus_tag="PK610_10835"
     CDS             3068..3277
                     /locus_tag="PK610_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRJ36476.1"
                     /translation="MEFPIQAPHEYIELNKLLKVLGLTETGGEANQCILDGMVLVNGE
                     VELQKRKKLRPGDRVEFDGEKVLIV"
BASE COUNT          924 a          713 c          817 g          978 t
ORIGIN      
        1 ccatcagcaa gggataaacg ccaagcagtt gaatgatatc agagccatag ggaacagttg
       61 catcggccgt cccatgaaaa ttaagtacag gttcatcacc ggctttgatc catgcagtat
      121 ctccaattgc accggcaatg tttacaatgg catgcacctt agatgaataa ccgggattac
      181 cacttaatcc aaccaaaccg ccatttagtc cgtattggtt tacggtgtcg gcttgtggtg
      241 gatattcgct catctgatca aggtaggcaa ggtgtagcgc cataaaaccg cctgcagaaa
      301 cgcctgcaaa gaagatttta gttgtatcaa tacggtaagg gtttccattt tcataactct
      361 ttctgaagaa gcggacagaa gcacgggcat cgtgtacagc gcgcattacg gattctgtag
      421 cgtctccgtc atcaggaccg ggaaaaggaa agttggtcat tcctacacgg taatcaattg
      481 aggaaacaac atatcccata cgggcaagat cttttgccat tggaagaaca tcacttcctg
      541 ttttgcttcc tcctaagaaa ttaccaccgt gagcgatgat aatcagggga cgttcagctt
      601 cggtatcacc ttttggaaag tgaatatcaa gattaagatt ttctgcttga ttcaatcggt
      661 ttacattcga gccgtactga atattcgata cggtggaatc agtaaagatc ttgtcgtgat
      721 accgctgtcc gcactgggca atcgccaatg catttagtga taaaaatcca gagaatagta
      781 acagtttttt catagggttg gggttagaaa acaaaagata agcttatacc gggaatgatg
      841 gcgcgtgatt tataggttcc gccaaaaccg gtttcaagat tggtgtcggt gcgtttcatg
      901 ccttcgatgt acaggaatga ggcgtcgatg ccaaatcctt ttttcaaacg aacacttgct
      961 cctgcagtaa ttccaatttt atttacatcc ggcgtttcgg gtgtgaggta accatcctgt
     1021 accggggtca tatcaaaata ggcgccgcct cgaatttgca actgatcatt taggcgatgt
     1081 tcatatcctg cgcggaaaat gaaactgttt ttatagttgc gtggcgaacg gttatccgca
     1141 agtttttcgc tgttgtcttt aaaatcaata acaagggaat cgtacgaact ccagcccaca
     1201 taatttacat caagcgctac acggtgtttg tctttggtgt agcctaatcc taacgaggct
     1261 gtaaatggaa gtcgaataga ggtgttaaat gttgtggtac caaaataaga attcaaagcc
     1321 ggagggacgc tgaattctgc ggagccgtcg atgatttttg cgagtatcga tgaacgaaaa
     1381 tcgatccctg cagaaaaacc atttttgcct ttgtaaaaaa caccggcatt gtaaccgaat
     1441 cccgaagctc ttccgttaag tcgtgcttca ccataagctc ctgtactgtc ttgcgcgggg
     1501 atgcctttac gcaaaccaaa tgttcctgtt gccagtataa atcccgctcc aataccaagc
     1561 tcatcgttta tcttgaacga aaagctgggc tgaaaacaaa ttgtcttcag gtcgatctca
     1621 cgaatgagaa attgccccac ccaatcgtct tcccattgtt gtttacttcc aaatggagtg
     1681 taaatgccaa atccgaaaga gagtcgtttg cttccttcaa gtcgtgatgc aacataaaaa
     1741 tgaaaaggtg ttccggtgtt gtgtaccgtt tccgttgtat agttcccggg aaagggggcc
     1801 agataacgta ccctcggaaa aagaagattt agtcctgcat tgatgttaag actcgaatcc
     1861 agaagagaga ttcctcccgg gttgaataaa accgtgcttg catcactgag taagcctgtg
     1921 ccggtatgac ccataccggt ttgtttttgt ccttgaagat tgagttgaaa acctcccgcc
     1981 tgtacaaaca ggggaagcat accaaagagg gcgagcagag atcttttcat gcagtgtgac
     2041 tgcaagaaag gtaataaaaa atccggaagt gacacaagca tgcagcactt ccggatgaaa
     2101 cactagagat ttcaggtaag cagaactttt ttggtaagac ggttttctgt gttttcgagg
     2161 cttacaaaat acacccctct cggaaggtcg ctaacatcca tgcgaattga tccgctaccg
     2221 ctttggtgac tgctgctgcg tacgcttttt ccactcaagt cgagcagttg aatggtgtac
     2281 atctgatcgt tggtcgattg cacaatcagc agatcggttg cgggattggg gtatactaca
     2341 aaatcgatgc tggcattgac tgtttcggta cgcgtgggtt gtgacatatc agtttcaatt
     2401 gcgatggctt ttgagtcggc ttcctgtgca tcggcatgcg gagcgcgttg ttcgtttccg
     2461 tctgccactt caatacttgt tgctttggga tctgccattt gtttgtcggt ttgagcaaca
     2521 cggttttgtc cgttcattcc accggaattt tcaatagatg tttttttagg atcggccatt
     2581 actttatctg aggcaccggc tttttgacca ggattagcct ttggtttgga ttttccattg
     2641 ggggtgccat tgtaaatggt ttgctctccc gcctggttgg tgggacgttg agcagatgat
     2701 tctgttgcca gtgcagctac aaaaatcaga aggaagattt gcgttttcat ggtattgctt
     2761 ttttgtttgg gttgggacat agtttttcct gccgggattt tcccggaaag gtgttgctgc
     2821 agtttttctc cgcaggggtt tcgttccatt tatcgcaacc ggaccaaaaa gagaaatgat
     2881 ggtccttatt ccgaatgccg tttgagagtt acggcatccg ctaaaaagcg ccttttgata
     2941 gtgagacgga gaagtaaagg attagttaca ggaaattttt aaatggtcgc agatcttgtt
     3001 gcatggcaaa aaaaataatg cgaagtgatg aacggtaagc aatgtatctt tgtggaaaat
     3061 aacagcgatg gaatttccta tacaagcacc tcacgaatac attgagttga acaaactctt
     3121 aaaagtatta ggacttaccg aaaccggtgg cgaagccaat caatgcatcc tcgatggaat
     3181 ggtgctggtg aacggagaag tagaattgca aaaacgcaag aagcttcgtc cgggagatcg
     3241 cgttgaattc gacggcgaaa aggttttgat cgtttgaatc tcctacaagg actgattttc
     3301 agtccttaag aaaatttcac aaggcttggc tcaatttgtc aatttgttta tatttgcaac
     3361 ccacaataac aatccttctt agctcagttg gttagagcat ctgactgtta atcagagggt
     3421 cgctggttca ag
//
LOCUS       DAOMYG010000355         3422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_9971_length_3422_cov_6.563410, whole genome shotgun sequence.
ACCESSION   DAOMYG010000355 DAOMYG010000000
VERSION     DAOMYG010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9971_length_3422_cov_6.563410"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..619)
                     /locus_tag="PK610_10840"
     CDS             complement(83..619)
                     /locus_tag="PK610_10840"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRJ36477.1"
                     /translation="MALQIGDRVTFLNEAGNGIVRALTVKGKALVETADGLEIEYPLK
                     YLVKMPDETDYSLPHANEQAFIRGKLRSENKAKKKTTVREKILEIDLHIYELTDNHRG
                     LSNADMLRMQLQAFKRKMEEAQRKRVQKLIVIHGVGEGVLRSEIRTALRQYENIEVLD
                     ADYAKYGRGATEVRFFGV"
     gene            complement(796..954)
                     /locus_tag="PK610_10845"
     CDS             complement(796..954)
                     /locus_tag="PK610_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HRJ36478.1"
                     /translation="MGKGDKKSTIGKRWRGSYGNTRPRKKKTVAAAPKKAKAAKKPAA
                     KKASKKAE"
     gene            complement(1091..1699)
                     /locus_tag="PK610_10850"
     CDS             complement(1091..1699)
                     /locus_tag="PK610_10850"
                     /inference="COORDINATES: protein motif:HMM:NF025718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2-related protein"
                     /protein_id="HRJ36479.1"
                     /translation="MEQWKNYLQTHRVLVMLSIVWFGTSLFLFNRVLEYIQNEPGIPF
                     NDPAFALYQAKDLSLFIFILTYGSLITFLVVNRKQPHHYILCLQAYGWMVVLRAISVY
                     LLPLAEPQEAIPLNDPVLNTFFYPGGYSPRDLFFSGHTGTIMLFAYLTDSKTIKILLY
                     VIALFTGFLLVLQKVHYSIDVLAAPVLMVPVFAIIKAQRKIN"
     gene            complement(1773..2177)
                     /locus_tag="PK610_10855"
     CDS             complement(1773..2177)
                     /locus_tag="PK610_10855"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HRJ36480.1"
                     /translation="MELLHIEATEKTPLVKFDPANGAFEIRGKSIPQDADSFYIPILE
                     WMEEYVNNPNSITKVVLDLEYFNISSSKRILFILYKLNELIDKGTDVKVDWYYIDQDD
                     DMKEVGQDFAFMVRVPFSFIAHKRYSPQMASA"
     gene            complement(2292..3020)
                     /locus_tag="PK610_10860"
     CDS             complement(2292..3020)
                     /locus_tag="PK610_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ36481.1"
                     /translation="MKAIIIDDERLARKELRSLLNKYHEIEIVDECANAGEALSSIDN
                     HHPELIFLDIQMPGHNGFELLNQLDHVPKVIFVTAYDEYAIRAFEVNALDYLLKPVNP
                     ERLDEAIAKVSRSKSSFSQSIPTEEATHKLTENDQIFLKDGEKCWFTSLKDVRMFESE
                     GNYVRVYFNEYKPLVLKSLNSLEERLDPKVYFRANRKFIINLKWVSGIENWFNGGLQV
                     QLRGGDKIEISRRQAARFRDLMSL"
     gene            complement(3023..>3422)
                     /locus_tag="PK610_10865"
     CDS             complement(3023..>3422)
                     /locus_tag="PK610_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRJ36482.1"
                     /translation="LSLVEDYLRLEQVRYEERLRAKVEMDPSCIGCLIPPLMVQTLVE
                     NGIKHGISKLPLGGEINVIVSRTDRMLSISVENTGTLSGSDNPQSTGIGIANTRQRLE
                     LIYEGKATFDLFASDGKVHAHITLPIQNNL"
BASE COUNT         1018 a          736 c          648 g         1020 t
ORIGIN      
        1 aggatccgga ttatttgaaa ttcctgaaaa gaaaaaggga gttaaaaagt aaacggatta
       61 accgtattac taatttcttc atttacacac cgaaaaagcg cacctcggtt gctcctctac
      121 cgtatttggc ataatcggca tccagcactt caatgttttc gtactgacgc aatgctgttc
      181 tgatttctga acgtaacacc ccctcaccca ctccgtgaat aacgatcaat ttctgaacac
      241 gtttacgctg tgcttcttcc attttccgtt taaatgcctg caattgcatt cgcaacatat
      301 ctgcattgct taatccgcgg tgattatcgg tgagctcata aatatgcagg tcgatctcca
      361 gaattttttc ccgtacagtc gttttctttt tggccttgtt ctccgagcgc aatttccctc
      421 gaatgaaagc ttgttcattg gcgtggggta atgaataatc ggtttcatcc ggcatcttta
      481 cgaggtactt caggggatat tcaatttcta acccatctgc agtctccacc agcgccttcc
      541 ccttgacggt aagcgctctt acgattccgt tgccggcttc gttaaggaat gtaacgcgat
      601 caccaatttg aagagccatg aatcctgaaa ttataacgaa aggggaaagt tttaattgac
      661 tgaaataaaa gaggatcgaa tggacccgga aggtatgatc acgtagtggg acaataccct
      721 tgtccctgat tttatcaaag aaaaaatccc ctgaaaccgg gaatctacaa atcaccggtt
      781 tcaggggatg gttttttatt ctgctttttt acttgccttt ttagcggcag gcttttttgc
      841 agctttcgcc tttttaggag cagcagctac agttttcttt ttgcgtggac gtgtattccc
      901 gtaagaacct ctccaacgtt tgccaattgt ggacttttta tcaccttttc ccataatggt
      961 atgttttact tggttatgat gtaaaaataa ccatttagct gaacgaacaa aatttagaca
     1021 aacaatactt gtcgggagtt gaactgtatt tctatttttc ccttttcctg cgcaaaaaaa
     1081 acagacagaa tcagttgatc ttacgctggg ccttaattat tgcaaatacc ggcaccatta
     1141 gaacaggagc agcaaggaca tcgattgaat aatggacttt ttgcagaacc aacaggaatc
     1201 cggtaaatag agcaattaca taaagcagaa ttttgattgt ttttgaatcg gttagatagg
     1261 cgaataacat aatggttcca gtatgaccgg aaaagaacaa atcgcgcggt gaatatccgc
     1321 ccggatagaa aaacgtgttt aagacaggat cattcaaagg aattgcctcc tgtggttctg
     1381 ccagaggtaa caggtaaacg ctaatagcac gtaaaacgac catccagcca taggcttgta
     1441 aacagagaat ataatgatgc ggctgttttc tgtttactac cagaaaagta atcagtgaac
     1501 cataggtcag aataaaaatg aacaatgaaa gatctttagc ctggtataat gcaaaggccg
     1561 gatcattaaa cgggataccc ggttcatttt gtatgtattc cagaactctg ttgaataaaa
     1621 acagggaagt tccaaaccaa acaatactaa gcattaccaa tacccggtgc gtttgcaaat
     1681 aattcttcca ttgttccatt cccgaatata catcaaaaaa aaagcgactc tttcgaatcg
     1741 cctgattaac ctaaaccaac ctgaaaatct cctcaggcag atgccatttg aggagaatat
     1801 cgtttatgag caataaaaga aaacggaacg cgtaccataa atgcaaaatc ctgccctact
     1861 tctttcatat catcatcctg atcaatgtaa taccaatcaa cttttacatc agttccttta
     1921 tctatgagtt catttaattt ataaagaatg aacagaattc gttttgacga agaaatattg
     1981 aaatattcca aatcgagcac cactttggtt attgagttgg gattatttac atactcctcc
     2041 atccactcaa gaataggaat gtaaaaagaa tcggcatcct gcggaatgga tttacctctg
     2101 atttcaaacg caccattcgc cggatcaaat ttgacaagcg gtgttttctc tgtagcttca
     2161 atgtgtagca attccataag tcctccaact ttgcacctct aatttaagca atcatttaac
     2221 cgtttttggt cagttttcta ccaataagga aaaagccccg accaatcggc ctgcataaac
     2281 aggtgaatgc cttacaagct cattaagtcg cgaaacctgg cagcctgccg acgggaaatc
     2341 tcaatcttat caccacctct aagctggacc tgtagtccgc cattgaacca attttctatt
     2401 ccgctaaccc atttgaggtt aatgatgaac ttacgatttg ccctgaaata cacttttgga
     2461 tcaagacgtt cctccagact attaagactt ttcaatacca atggtttgta ttcattgaaa
     2521 tacacacgaa catagttgcc ttccgactca aacatccgaa catccttaag agaagtaaac
     2581 cagcattttt cgccgtcttt caaaaagatt tgatcattct cggtgagttt atgcgttgct
     2641 tcttctgtgg gaattgattg actgaaacta ctttttgaac gggaaacttt cgcaatggct
     2701 tcgtccaatc gttccggatt aaccggtttg agcagataat caagcgcatt cacttcaaat
     2761 gcacgaattg catattcgtc gtacgcagtt acaaaaatca ctttgggtac atgatcgagt
     2821 tgattcaata actcaaatcc gttatgtccc ggcatctgaa tatcaagaaa tatcaattcc
     2881 ggatgatgat tatcaatact gctcagtgct tcgccggcat tggcgcattc atcaacaatt
     2941 tcaatttcgt ggtacttgtt gagcagagaa cgcaattcct ttctggcaag acgttcatcg
     3001 tcgattatga tcgctttcat attcaaagat tgttttgaat gggtaaggta atgtgagcat
     3061 ggacttttcc atcggaagca aaaagatcaa atgttgcttt tccttcgtaa ataagttcga
     3121 gtcgttgacg cgtatttgca attccaattc cggttgattg tggattatca cttccggaaa
     3181 gtgttcctgt attttcaact gaaatggaaa gcatacgatc cgttcgtgaa acaatgacat
     3241 ttatttctcc acccaaagga agtttggaga tcccgtgttt aattccgttt tcaacaaggg
     3301 tttgcaccat aagcgggggg attaaacaac caatacagga gggatccatt tccactttgg
     3361 cacgtaagcg ttcttcatag cgcacctgct ctaaccgaag ataatcttct acaagtgata
     3421 at
//
LOCUS       DAOMYG010000356         3398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10066_length_3398_cov_8.021837, whole genome shotgun sequence.
ACCESSION   DAOMYG010000356 DAOMYG010000000
VERSION     DAOMYG010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10066_length_3398_cov_8.021837"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PK610_10870"
     CDS             <1..487
                     /locus_tag="PK610_10870"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRJ36483.1"
                     /translation="VRKKDYSLFHYRKGELNSRFDICLGQDPQGHKIQQGDNKTPEGE
                     YRISEMEKGPFVGGTGPWLGERWMHFSYPNRYDAWLGYKNGIITRDQFLAMEKADKSG
                     KTANNYTKLGGRIGIHGWNGRFVANGTQDLTWGCVCMQNEDIVQLYEQIKLGVKVMII
                     P"
     gene            complement(559..2568)
                     /locus_tag="PK610_10875"
     CDS             complement(559..2568)
                     /locus_tag="PK610_10875"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ36484.1"
                     /translation="MKKRSTLLFLSLFTVGSLFAQFTDNFDSYTAGGYLGIQSGPWTT
                     WSGAQGGAEDVQITTADANSAPNSVLFTSTAAAGGPQDVVLPFGGVRTTGQFSIGMAI
                     KVQTNKGAYFNLQADATIGQTWALECQMLQNGNMTFGNTNGQLLATTYTPNTWFDIRI
                     DIDLNANNWEVFINNVSQGSFSNPINRVSFLNIYPVNQTAAGGNGQSGFYVDDVFYTH
                     TPYTLPAVNGAVTLVSVPSGLATQQRNVSASIRNLGTTTITSFDINYNYNGNNSNQSV
                     TGLNLASLATYQYTFPTQITLVAGSNPVTVTISNVNGAGADGDANDNVKVLTINPVVP
                     AAGKMVVGEEGTGTWCGWCPRGAVFMDMMASKYDGFWAGIAVHNGDPMVVSDYDAAIG
                     GLIGGYPSALVDRGTDIDPSQMEAAFLSRVVLAPAATFAMNGSFNSSNSQLTVNVTTT
                     FQTATSGDWRILMVLTEDDVTGTGSGYNQTNYYAGGSNGVMGGYEALANPVPAAQMHY
                     EHVARDIEPSFTGIQNAFTASIAQNDAFNNTFNFTINAAWELNHMHAIVILRHPNGSI
                     ENAGMLDLSSLVTGVDDNASSVNARLYPNPAADLSQVEINLDVNSTVSMEVYDLQGKV
                     IATRDYGVLNGKQLLPIHTSAWEQGVYLVRLTVNGEVKSFRLIVQ"
     gene            2710..>3398
                     /locus_tag="PK610_10880"
     CDS             2710..>3398
                     /locus_tag="PK610_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36485.1"
                     /translation="MVEPDFNSMTRIDWLGFTLLEPFTVLTDLVITAVCIFGWFKLKE
                     RSKTEKVIWLLRWFLLTMGLATMVGGVLGHGLLYLTGMKGKIPGWYISMVSVAFFERA
                     VIMHARPVLNKGMGNFFSWLNYVELAAFMVLAAVTLKFIYVELHAVYGLFIMAFSLEI
                     FVYVKTRDKGSVYFFIATFFAAASAVSHAFRIGINEWFNHNDVSHIGMAIAVYFYYLG
                     GKHLTVHLRKE"
BASE COUNT          996 a          672 c          756 g          974 t
ORIGIN      
        1 ggtgcgcaaa aaggattatt ccctttttca ttaccggaag ggtgagttaa attcacgttt
       61 cgatatttgt ctgggacagg atcctcaagg gcataaaatt cagcagggcg ataacaaaac
      121 acctgagggt gagtacagaa tttcagaaat ggaaaaaggt ccttttgttg gaggtacagg
      181 tccatggtta ggtgagcgtt ggatgcattt cagttatccc aacagatatg atgcgtggtt
      241 gggttacaaa aacggaataa ttacccggga tcaatttctt gccatggaaa aagcagataa
      301 gagcggaaag acggctaaca attataccaa actgggtggc agaataggca ttcacggatg
      361 gaatggaagg tttgttgcca acggaaccca ggacctaacg tggggatgtg tctgcatgca
      421 aaatgaagac atcgttcaat tgtacgagca gattaagctg ggcgttaaag tcatgatcat
      481 tccctgatag tgtgctgttt ctactccaaa aaaaagccgc atccaaatca ggatgcggct
      541 ttgttgttgt atggacgatt attgaacgat caatcggaaa ctttttactt caccattcac
      601 cgttaaacgc acaaggtaca caccctgttc ccaagctgaa gtatgaatag gcagcaattg
      661 cttaccattc agaactccat agtctcttgt agcgattact ttgccttgca aatcataaac
      721 ttccattgat acagttgaat tcacatccag attgatttca acctgagaaa gatctgcagc
      781 aggattaggg tacaatcggg catttacaga agaagcattg tcatcaactc ctgtaaccag
      841 actactcaaa tcgagcattc cggcattctc aatagagccg ttcgggtgac ggagaattac
      901 aattgcatgc atgtggttga gttcccaggc agcattgatt gtaaaattga acgtgttatt
      961 gaatgcatca ttctgggcaa tgcttgccgt aaatgcgttc tgaattcctg taaacgaagg
     1021 ttcgatatcg cgggcaacat gctcataatg catttgtgca gcaggaaccg gattcgcaag
     1081 agcttcgtag cctcccatta cgccattgct tccaccggca taatagttgg tttgattgta
     1141 tccgcttccg gtaccggtaa catcatcttc agtaagcacc attagaattc tccaatctcc
     1201 ggaagttgcg gtttggaagg tagtagtaac attaactgta agctgagaat tcgaagaatt
     1261 aaaagatcca ttcattgcaa aagttgcagc gggagcaagt accacacgac ttaaaaatgc
     1321 cgcttccatt tgagaaggat caatatcagt accacgatca accaatgcag aagggtaacc
     1381 accaattaag ccacctattg cagcatcgta atcagatact accattggat ctccattatg
     1441 cacagcgata ccggcccaaa agccgtcgta tttggaggcc atcatatcca tgaaaacagc
     1501 accacgcgga caccagccgc accaagttcc tgttccttct tcaccgacaa ccattttacc
     1561 tgctgcaggt acaacaggat taatggttaa cacctttaca ttatcgtttg catcaccatc
     1621 tgctcctgca ccatttacat ttgagatggt aacagtaaca ggattactac ctgcaaccaa
     1681 tgtgatttgg gtcgggaaag tatactgata agttgcaagt gaagcaagat tcaaaccggt
     1741 aacagattga ttggaattgt taccgttata gttgtaatta atatcaaatg aggtaatggt
     1801 agttgtaccc agattacgta ttgaagcgga tacattgcgt tgttgcgttg ccagaccgga
     1861 cggaacactt acaagcgtaa ctgcaccatt aactgcaggt agcgtgtatg gagtatgtgt
     1921 gtaaaacaca tcgtccacat aaaacccgga ttgaccgttt ccacccgcag cagtttgatt
     1981 cacggggtag atattcaaaa aggaaactct gtttatcgga ttggaaaatg aaccttgaga
     2041 aacattattg atgaacactt cccaattgtt ggcattcaaa tcaatatcaa tacgaatatc
     2101 aaaccaggtg ttgggggtat acgttgtagc aagcaattga ccatttgtat taccaaaggt
     2161 catatttcca ttctgcagca tctggcactc aagtgcccag gtttgtccga tggtagcatc
     2221 tgcctgtaag ttaaaatatg ctcccttatt ggtctgaact ttaattgcca tgccaatgga
     2281 aaactgacct gttgtgcgga ctccgccaaa cgggagaaca acatcctgcg gaccaccggc
     2341 tgcagcggta gaagtaaata aaacggagtt gggtgcactg tttgcatcgg cagttgtaat
     2401 ctgaacatcc tctgccccac cctgagcccc gctccaggta gtccatggac cagactgaat
     2461 gccaaggtaa ccaccggcag tatacgaatc gaaattatcc gtgaactgtg caaacaagct
     2521 tccgactgta aaaagcgaaa ggaataataa agtagagcgt tttttcataa aagagatttg
     2581 agcgatcaat atgagaagat taaagtaagc ataaaaacca agaaatggca attctcgtat
     2641 tcctaattag gaattatgaa tcgtttagtt ttcatttagt accttaattg ccagttaaat
     2701 tttactgtaa tggttgagcc tgattttaat tcgatgacgc gtatagactg gctggggttt
     2761 accttacttg aaccttttac tgtgcttact gatttggtca ttaccgctgt ttgtattttc
     2821 ggttggttca agctgaaaga acgttcaaaa accgaaaaag taatatggct attgaggtgg
     2881 ttcttactaa caatgggact tgcaacgatg gtaggtggtg tattgggtca tggattgttg
     2941 tacttaaccg gtatgaaagg gaaaataccg ggttggtata tcagcatggt ttctgttgca
     3001 ttttttgagc gtgctgtaat catgcatgct cggccggttt tgaataaagg aatgggtaat
     3061 tttttctcct ggttaaatta tgttgaattg gcagcattta tggtattggc tgctgttaca
     3121 ctaaagttta tttatgttga gctacatgct gtttacggac ttttcattat ggctttttca
     3181 ttagagattt tcgtttatgt gaaaaccaga gacaaaggca gcgtttactt ttttattgcc
     3241 actttttttg cggctgcttc agctgtatcc catgccttcc gaattggtat taatgagtgg
     3301 tttaaccata atgatgtgag tcatatcgga atggctatag ctgtatattt ctattattta
     3361 ggtggaaaac atttaactgt tcacttgaga aaagaata
//
LOCUS       DAOMYG010000357         3390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10111_length_3390_cov_8.443478, whole genome shotgun sequence.
ACCESSION   DAOMYG010000357 DAOMYG010000000
VERSION     DAOMYG010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10111_length_3390_cov_8.443478"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="PK610_10885"
     CDS             complement(<1..1239)
                     /locus_tag="PK610_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRJ36486.1"
                     /translation="MQEAKPYQPKNKVRIVTAASLFDGHDAAINIMRRIIQSTGAEVI
                     HLGHDRSVEEVVNCAIEEDAHAIAMTSYQGGHTEYFKYMHDLLKEKGAGHIRIFGGGG
                     GTILPSEIEELQHYGITRIYHPDDGRSMGLQGMINDLMQKSDYAIGDHLNGEVKQLSP
                     ANKNSVARLISAAENFPDQSRAVLDAIAEQAKKSKSPVLGITGTGGAGKSSMVDEIVR
                     RFLLDFPDKHIGIVSVDPSKRKTGGALLGDRIRMNSIRNPRVYMRSLATRQSNLALSR
                     YVGDAVNILKAANYDLIVLETSGIGQSDTEILDHSDVSLYIMTPEFGAATQLEKIDML
                     DFADVVAINKFDKRGALDAIRDVKKQYKRNHQLWDADDETLPVYGTIASQFNDPGTNR
                     LYKAIMDKVVEKTNAPLKSGF"
     gene            1458..1670
                     /locus_tag="PK610_10890"
     CDS             1458..1670
                     /locus_tag="PK610_10890"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ36487.1"
                     /translation="MYNMVLSNNRAKAAVDYLISQGIDPSRLKWKGYGESKPRNKCVN
                     GVVCSEEEHQWNRRTEFKVTKILPEK"
     gene            complement(1703..2104)
                     /locus_tag="PK610_10895"
     CDS             complement(1703..2104)
                     /locus_tag="PK610_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36488.1"
                     /translation="MIKRLLIVSLLLFLFKPGELRAQLIADYLIKGADWRMCGSHQDK
                     DTLRFFRYDAKVKGTQCFQQTYTQLLTYSPNEFRILNWTNGACNINEPGTFEANDSNG
                     TIRFYFDDGSSRDYLMLFLNKEKLVLVDLKY"
     gene            complement(2104..2841)
                     /locus_tag="PK610_10900"
     CDS             complement(2104..2841)
                     /locus_tag="PK610_10900"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ36489.1"
                     /translation="MKTIAVFNIKGGVGKTATAVNLAYAASEAKNKTLLVDLDPQGAA
                     SYYMHHEEGIENAKKIIAGEKSPEEAILQTIYPNLWLLPADERYRKMDVFLSELKGGR
                     SWMKSLFKPLSRKFDVAIIDCPPSITLFSENILLNADYILVPVVPSTLSVRTYEQLID
                     YCKVNKIDTKKIHPFFSMYERRKNLHNETIVEFMSVHQEAIDVAIPFVSEIERMGVYR
                     RPYLDAHPETEISYLYRQLWKSVKKKL"
BASE COUNT          987 a          727 c          715 g          961 t
ORIGIN      
        1 aaatccggat tttaagggag catttgtttt ctccactacc ttgtccataa tcgccttgta
       61 caaacgattg gttccgggat cattgaactg agaagcgatg gtaccatata ccggcaaggt
      121 ttcatcatcc gcatcccata actggtgatt acgtttgtat tgcttcttta catcgcgaat
      181 ggcatcgagt gcaccgcgct tatcgaattt gttgattgca actacatccg caaaatcaag
      241 catatcgatt ttttccaact gtgttgccgc accaaactcg ggtgtcatga tgtaaagcga
      301 tacatccgag tgatcaagaa tttcggtatc gctctgacca atacccgaag tttccagaac
      361 gatcaaatcg tagttagcag ctttcagaat atttactgca tcaccaacgt acctactaag
      421 agccagattg ctttgtcgtg ttgccagcga tcgcatatac acgcgtggat tgcgaattga
      481 attcatccgt atacgatctc ccagcaatgc tcccccggtt ttacgtttgg aaggatcgac
      541 tgaaactata ccgatatgtt tatccggaaa atcaagaagg aaacgacgaa caatctcatc
      601 aaccatactc gatttaccag ctcctcctgt tcctgtaata cctaataccg gcgattttga
      661 ttttttagcc tgctcagcga ttgcatcaag aacagcacgt gactgatcag gaaaattttc
      721 tgcagcggat attaatcgcg ctacactgtt tttattagca ggtgaaagtt gtttgacttc
      781 tccgttcaga tgatctccaa ttgcataatc acttttctgc atgagatcat tgatcattcc
      841 ctgcaatccc atcgagcgtc catcgtcggg atgataaata cgtgtgattc cgtaatgctg
      901 caattcttca atctccgagg gaaggatggt tcctcctcct ccaccaaata tgcggatatg
      961 gcctgctcct ttttccttaa gcagatcgtg catgtatttg aaatattcgg tatgacctcc
     1021 ctgataactg gtcattgcaa ttgcatgggc atcttcttca atggcgcaat tcacaacttc
     1081 ttcaacagaa cggtcgtgac caagatgaat aacctcagcg ccggtacttt gtatgatacg
     1141 acgcataata ttgatagcag catcatgtcc gtcgaaaagc gatgcggcgg ttacgatgcg
     1201 gactttattc tttggctgat agggttttgc ttcttgcatg aggtatcggt ttcacaggca
     1261 caaatataac aaaagaggag gctgaagatt cgcctttgat tgccgattgc actactataa
     1321 atcaacatat tacataccaa gcccgggata ttttctttac aattgccccg caatagcact
     1381 acgctacaac aggaatgaaa caatccaaac aacaataata taactcaaca tcctctaatt
     1441 attgcagagg aaaagacatg tataatatgg ttttatccaa caatcgtgcg aaagcagcag
     1501 tcgattatct catttcgcag ggtattgacc catcacgctt gaaatggaaa ggctacggag
     1561 aaagcaaacc acgcaacaaa tgtgtaaatg gtgttgtttg ctccgaggaa gaacaccaat
     1621 ggaacagaag aaccgaattt aaggtcacca aaattttacc tgaaaaataa atgcaagccc
     1681 cggagcagaa aagtttcggg gcttagtatt tcagatcgac aagcacaagc ttctccttgt
     1741 tgagaaataa catcaggtaa tcgcgggaag agccatcatc gaaataaaac cggatagtcc
     1801 cgttactgtc gttggcttca aaagttcccg gctcgttaat gttgcatgca ccgtttgtcc
     1861 aattgaggat tcgaaattca tttggactat aggtgagcaa ttgcgtatac gtttgctgaa
     1921 aacactgtgt gccttttact tttgcatcgt accgaaaaaa acgaagtgtg tctttgtcct
     1981 gatgcgaacc gcacattctc cagtcggccc ctttgatcag gtaatctgca atcaattgag
     2041 cgcgcagttc accaggcttg aataaaaaca gtagcaatga aacaatcagc agtcgtttga
     2101 tcattacaat tttttcttaa ctgatttcca taactgacgg tacaaataac tgatttccgt
     2161 ttcaggatga gcatccaggt aaggacgacg gtaaacaccc atccgttcaa tttcagaaac
     2221 gaaaggaata gccacatcaa ttgcttcctg atgcaccgac ataaattcca caattgtttc
     2281 gttgtgtaaa tttttgcgtc gctcgtacat tgagaaaaag ggatggatct ttttggtgtc
     2341 tattttattc accttgcaat aatcgatcag ctgttcataa gtacgcacag aaagtgttga
     2401 gggaactacc ggtaccaaaa tataatcggc gttaagaaga atattttccg aaaagagagt
     2461 gatgcttggc gggcagtcga taatggcgac atcgaatttg cgggaaagtg gtttaaacaa
     2521 ggatttcatc catgatcttc cacctttcag ttcgcttaga aacacatcca ttttccgata
     2581 gcgttcatca gcaggcaaga gccatagatt gggatagatc gtctgaagaa ttgcttcttc
     2641 cggacttttt tcaccggcga tgattttttt tgcgttttca attccctctt cgtgatgcat
     2701 gtaatacgaa gcggccccct gcggatcgag atcgacaagt agtgttttgt ttttggcctc
     2761 agacgcagca tacgctaaat ttacggccgt ggcagttttg cctacaccgc ccttaatgtt
     2821 aaatacggct atcgttttca tacaggtttt ggaatggata gtcttcaaat ataatgaaaa
     2881 tcagcacttt tccaaatcgc acaccactag cctaacaaac ctaaatgact gaatcacata
     2941 aagatacagc aaacaacccg aaaataagca ctgatacttt tgagcttcat tacctgcgtt
     3001 tgggctacac aatcaagggc gatagtattt tttggaagga aggaacaggt tgtttgatta
     3061 aggattttaa gaaaggggag aatcccggcg ctgtggtcag gaaaggcggg aatgggatga
     3121 ttttgatcga gggtaatcaa aacgggatac acaaggctaa aaccaattca ggtttatggt
     3181 taatgttttt tgcttgtgtg tttttgggaa ttctatttct gattgtcaga aaaaaggcac
     3241 ttaaaactcg taggaaaaaa cgttggagat aaagtaggtt gtttccggag caccccgtgg
     3301 aggacgcgaa tcgtgttgca gattgtacac aagccgaaag cgcaattttt gtgtgatatt
     3361 aaatcgcaag gaaacttcag tcgacaccct
//
LOCUS       DAOMYG010000358         3367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10228_length_3367_cov_9.949577, whole genome shotgun sequence.
ACCESSION   DAOMYG010000358 DAOMYG010000000
VERSION     DAOMYG010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10228_length_3367_cov_9.949577"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PK610_10905"
     CDS             complement(<1..435)
                     /locus_tag="PK610_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36490.1"
                     /translation="MNRHSFSYLFLLFSSTCLSAQDLSYEDALRITAKVEQEQRYVGA
                     LSEHDLHQLPVGIIKQVGSSRYVIAIDSAIFESDKAYFHAYMAVEFPGTGKVIAFGAR
                     RVHFNPKGIGGGTDSRLMLLSDHSIPIGPNSRLELRGNGSNYV"
     gene            complement(512..943)
                     /locus_tag="PK610_10910"
     CDS             complement(512..943)
                     /locus_tag="PK610_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HRJ36491.1"
                     /translation="MSDGRISSSKAPKPVGLYPHARRVGNLLFLSGVGPRVANSNAND
                     STVPGLKLDHNGNFEEFDFEAQVHSVFQNVKAVLEDSGSSWDKIVDVTVFLVHMKRDF
                     RTFNRIYAEYFKDNLPCRTTVEINSLPTPIAIELKVIATID"
     gene            1042..1860
                     /locus_tag="PK610_10915"
     CDS             1042..1860
                     /locus_tag="PK610_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36492.1"
                     /translation="MLTIPRLFLYLHAGFLLIACSGKNDNAPDTKSENDSTTAITKAD
                     SLLDLNGELRSLALGFTEITSFPLVLDTAITNHFDDYDSLPGDDARMLFSKAPQHNLN
                     ETPAYLFDYFLKIDSLKRAGEYSAYVESLDIGMIKEARTKKLFRVRLHPETLLIVWGM
                     DYHSYEACPWSRGSFVFVTIVHKGEPGETLCLSLNDAGGDPPVSGNTERQATIQEDGS
                     ISIHQKDISDEDMDEPEVSLKESSFEFMITKNVFRHVRSKEGKDVRVKREEVGY"
     gene            1940..3124
                     /locus_tag="PK610_10920"
     CDS             1940..3124
                     /locus_tag="PK610_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36493.1"
                     /translation="MLKSISPYLCAAAVFLVSCGTESNETSGKNDTLVKDSAAIETPN
                     TPEKIAYDTLLTETSRFIAGLEASIHFGEHQQKEFYLKHKDFTDKSWTTTLDSMIRPV
                     QKWCAEKNIGDNRDSVLCFYPLSGPDFLFGNTFFPNADHYVLLGLEPRGYLKDMRIMS
                     DNELMKYFDGIRQSMKYINSRGYFVTSHMSSDFTKAHLNGMVHMMLYMMARTEHKILD
                     VYEVWIDEKGKEQRLSEGEKAPEGKITASKIEFLSPDQTEKRDAYYFKLDASDKYLQD
                     HTEFSDFINGFGPRVAYMKSASCVLQNTPFAIMRKLVIDSDKILQDDTGVPYKYFSED
                     STLRVQLFGTYSKTIDDLSWCYQPSLRKALETSGNNSDLPFRISYNGNYGEGMLLWAV
                     RK"
BASE COUNT          986 a          714 c          719 g          948 t
ORIGIN      
        1 cacataattg cttccgttac cccgcaattc aagcctgctg tttggtccaa ttggtatgct
       61 atgatcactc agcaacatca gtctcgaatc tgtcccgcca ccaattcctt tcggattaaa
      121 atgaactcgt cttgcaccaa atgctatcac cttccccgtt ccgggaaact ctacagccat
      181 ataggcgtga aaataagctt tgtccgattc aaaaattgcg ctgtcaattg caattacata
      241 gcgtgatgaa cctacctgct taatgatgcc tacgggtaat tgatggaggt cgtgttcact
      301 taatgcgcca acataacgct gctcttgttc cacctttgca gtaatacgta aagcatcctc
      361 ataactcaag tcctgagccg ataaacaagt actgctaaaa agaagaaaaa ggtaggaaaa
      421 cgaatgcctg ttcatggaac aaacatcgca tatagaaaaa acagttgcag accaatttag
      481 ggtccacttt tacagttggg tgagcccagt atcaatcaat ggttgcgata accttcagct
      541 caatggcaat gggtgtaggt aaagaattga tctcaacagt tgtgcggcaa gggaggttgt
      601 ctttaaagta ttcagcgtaa atcctgttaa aggttctgaa gtctcttttc atgtggacaa
      661 gaaaaacagt gacgtcaacg atcttatccc acgaagagcc ggaatcttcg agaacggcct
      721 ttacattctg aaaaactgaa tggacttgcg cctcaaagtc aaattcttca aaattcccgt
      781 tgtgatcgag tttcaatccg ggaacagtgc tgtcattggc gttgctattg gccaccctcg
      841 gaccaacacc gctgagaaat agcagattcc caacacggcg agcatgaggg tataagccta
      901 ctggtttggg tgcttttgaa gaggagatac ggccgtctga catgggtttt caattaggac
      961 tgcaaattgc tcattattcc ttaaaaatga aaatcacttt attcgaattg taggcctatt
     1021 tttgtgcttc aaattattgc catgctcaca atcccaagac tatttctcta tctgcatgcc
     1081 ggtttcctgt taattgcctg ctctggaaag aacgataacg cacctgatac gaaaagcgaa
     1141 aatgacagca caacagctat tacaaaagcc gattctttgc tcgatttaaa cggtgagctc
     1201 agaagtcttg ccttaggatt cactgaaatt acctcctttc ctcttgtgtt ggatactgct
     1261 attaccaatc attttgacga ttacgattca ttgccgggtg atgatgcgcg catgttgttt
     1321 tcgaaagcac cacagcataa cctcaacgaa accccggcgt atctattcga ctactttttg
     1381 aaaatagatt cattgaaaag agcaggtgaa tattccgcct atgtagaatc gcttgacatc
     1441 ggtatgatca aggaagcccg aaccaaaaaa ttgttccgcg ttcgccttca tcccgaaacg
     1501 ttgctgattg tttggggaat ggattatcat tcctacgagg cttgtccgtg gagtcggggc
     1561 agttttgtat ttgttacgat tgtccataaa ggcgagcccg gggaaacctt atgtctgtca
     1621 cttaacgatg cgggaggaga ccctccggta tcgggcaaca ctgagcgtca ggccaccatt
     1681 caagaagatg gaagtatcag tattcatcaa aaagacatca gtgatgaaga tatggatgag
     1741 cctgaagtta gcctgaagga aagtagcttt gagtttatga taactaaaaa tgttttcaga
     1801 catgttcgaa gcaaagaagg taaagatgtt agggtgaaaa gagaagaagt aggttattaa
     1861 cctccctatg ctgaatattt gttcctcatc atctattatc cgtcacaagg aatgacgcgc
     1921 caatatctat ctttgaacca tgcttaaatc gatttcccct tatttatgtg ctgcagcagt
     1981 atttcttgtt tcgtgtggaa cagaaagcaa tgaaaccagc ggcaagaacg acactttagt
     2041 aaaggattct gcagcaattg aaacgccgaa tacaccggaa aagattgcat acgacacctt
     2101 gcttacagaa acttctcggt tcattgcagg cctggaagct tctattcatt ttggagagca
     2161 tcagcaaaag gaattttatc tgaaacataa agattttacc gacaaatcct ggaccacaac
     2221 tctggatagc atgattcgtc cggtgcagaa atggtgcgct gaaaaaaata taggcgataa
     2281 ccgcgattct gtattgtgtt tttacccatt atcgggaccg gattttcttt ttggaaatac
     2341 atttttcccg aatgcagatc attatgtgtt gcttggactt gagccgcgtg gttatttaaa
     2401 ggacatgcgc attatgagcg acaacgaact gatgaaatat ttcgacggga ttcgccaaag
     2461 catgaagtac atcaacagca gaggatactt tgttacctcg catatgagtt cagatttcac
     2521 caaagcccat cttaacggaa tggtgcatat gatgctgtat atgatggcac gcactgagca
     2581 caagattctg gatgtgtatg aagtttggat cgatgaaaag ggaaaagagc aacgactttc
     2641 tgaaggagaa aaagcgcctg aagggaaaat aacagcgtcg aaaattgaat ttctgtcgcc
     2701 cgaccaaacg gaaaaaagag acgcgtatta tttcaagttg gacgcctcgg ataaatacct
     2761 gcaggatcat actgagtttt cagatttcat caatggattt ggtccgcgcg ttgcctatat
     2821 gaaatcagcc tcctgcgtat tgcagaacac gccctttgcc attatgcgta aactggtgat
     2881 tgattccgac aaaatacttc aggatgatac cggtgttccg tacaagtatt tttctgaaga
     2941 ctctactctt cgtgttcagt tgttcgggac gtatagtaaa accattgatg atttgagctg
     3001 gtgctatcaa ccctcgctga gaaaagcact cgaaacttcc ggtaacaatt cagatcttcc
     3061 cttccgcatt tcttataatg gaaattacgg cgaaggaatg cttttgtggg ctgtcagaaa
     3121 ataaaatttt cgttgcgcct ttgcaaactc agtgaattcg ttttatcgct ttgctgtttt
     3181 tgaaaccatc ctgctaccgc aattctgcga ctcccttttt tttcgaaatg aaggtgattc
     3241 ctccatgaat aagaacaaag catttgcatt cacctattta accacaatgc aattacaagc
     3301 catcggaaat tccgattgat ggcttaactt tgaaatgtgg acaaatcctt gcctatactt
     3361 gagatac
//
LOCUS       DAOMYG010000359         3364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10244_length_3364_cov_8.696585, whole genome shotgun sequence.
ACCESSION   DAOMYG010000359 DAOMYG010000000
VERSION     DAOMYG010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10244_length_3364_cov_8.696585"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1487
                     /locus_tag="PK610_10925"
     CDS             <1..1487
                     /locus_tag="PK610_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36494.1"
                     /translation="GEVLYNRLAYLGNEFPIEVTIDAGKCKSEKAEIKILKGNEPVAS
                     KVVQIDKDQFTTKVSFKLEARTPGIQRYRIAVTPLNDELTILNNHRDIFIEVLNSKQK
                     ILLLAHSPHPDLNAIRSAISENRNYEVSSRLISDFNEPINNYNLIILHQLPSLTNNAK
                     AILDQSENAGIPLLIVLGAQSSINAFNELKTGLTISNNRGTTGLAAGSFNTGFSLFQI
                     EEKGARQFLCYPPLQIPFGEWKSSQACEVLFNQRIGTVETQFPLVFFNKETRRKFGVI
                     AGEGVWRWKMADFAQNENNDNFNGMLQKSIQYLASRENKSFFRVFAQNEYPESENILI
                     NAELYNKSYELINEPEVEIIISNEEKKDFRYIFSRTATAYRLDAGKLPAGQYRYTAKT
                     EFNSAKYEVSGEFSITPVMLELAKSVADHQLLAALAFQTKGEMIHPNDIRSLPEKIGS
                     QEDIVEVVFPERRLDDLLHFRWIFFLILALLTLEWFIRRWSGAY"
     gene            1498..2268
                     /locus_tag="PK610_10930"
     CDS             1498..2268
                     /locus_tag="PK610_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36495.1"
                     /translation="MKSRLILALLFLLLDFSGSAQSFRLPDSWEPQDVLLVKTKGSHT
                     GSQTTIRFQSDQCTYEENLEGKSITIPFQMHPKTFEALLFYLKDRKFNELKFKKLKTI
                     TYDKNSSIIYWLRGGKQLFRIGYDADIEVHRNSEKRFNMILLHLIQLAEQHTEKLKVK
                     VNFTIHSSVKESMLDLNYRIDGSGQRAWFGNSQIPNMISLMLFRVRYSFHFALLDGAK
                     GLNGENPGTIWTLFEPDIANHTEFEILLERRRLVVRPL"
     gene            complement(2302..2988)
                     /gene="cmk"
                     /locus_tag="PK610_10935"
     CDS             complement(2302..2988)
                     /gene="cmk"
                     /locus_tag="PK610_10935"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRJ36496.1"
                     /translation="MKKINIAIDGYSSCGKSTMAKQLARHFGFVYVDSGAMYRAVTLF
                     CLRQGMVKDNFVMKDQLIECLDDIDIRFRFNHENQRSETFLNNENVEQEIRGMEVSRY
                     VSLVSLIKEVRVKLVALQQSMGQEKGVVMDGRDIGTAVFPHAELKIFMTADKETRARR
                     RYEELKAKGAKVSMDEVIENISSRDFEDTHRKENPLVQAIDARIIDNTMLSPEEQFDL
                     AKSWVEEKLG"
     gene            complement(3020..>3364)
                     /locus_tag="PK610_10940"
     CDS             complement(3020..>3364)
                     /locus_tag="PK610_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36497.1"
                     /translation="HRWDLSYLDPTLKPTIDPSTGEKIEPKAPGFGDKLMRHAVFGTE
                     LVTKNFFIGFGFNYRRRAELRVRERGGLSGFSFGMGMKIKKFHLSYALATYNQAGLSN
                     HITIAFNLNEFR"
BASE COUNT         1030 a          733 c          649 g          952 t
ORIGIN      
        1 ttggagaagt cctatacaat cgacttgctt atttgggcaa tgaatttccc attgaagtaa
       61 ctattgatgc cggcaaatgc aaaagcgaaa aagcagaaat caaaattcta aaaggaaatg
      121 agcctgttgc ttccaaagtt gttcaaatcg acaaggatca atttaccacg aaagtaagtt
      181 tcaaactcga agcaagaaca cccggaattc agcgttatcg gatagctgta acgccgttga
      241 atgatgaact cactattctg aataatcatc gggatatttt tattgaagtt ctgaacagca
      301 aacaaaaaat cctactcctc gctcacagcc cccatcccga ccttaacgcc attcgatcgg
      361 ccatcagtga gaatcgaaac tatgaagtaa gttccagatt gatcagcgat tttaatgagc
      421 ccataaacaa ttacaatctc attattctcc atcaattacc atcccttaca aataacgcaa
      481 aggctattct cgatcaaagt gaaaatgcgg gaattccatt gttaatcgtg ttgggtgctc
      541 aaagcagcat caatgcattc aatgaactca aaaccgggct taccattagt aataaccgcg
      601 gaacaaccgg actggccgcc ggcagtttta ataccggatt cagtcttttc caaatcgaag
      661 agaaaggtgc acgtcaattt ttgtgttatc ccccacttca aattcctttt ggcgaatgga
      721 aaagttcaca ggcctgtgag gtattattta accagcgcat tggaactgtg gaaacccaat
      781 tcccacttgt gttcttcaat aaagagacaa gaagaaaatt cggcgtaatt gcaggtgaag
      841 gtgtctggag atggaaaatg gcagattttg cgcagaatga aaacaacgat aattttaacg
      901 ggatgctcca aaaaagcatt caataccttg catcgcgcga aaacaaaagt tttttccgtg
      961 tttttgccca aaacgagtat ccggagagtg aaaatatcct cattaacgct gaactgtaca
     1021 acaaaagcta cgagttgatc aacgaacctg aagttgagat tatcatttct aatgaagaga
     1081 aaaaggattt caggtacatt ttcagcagaa cggcaacagc ctatcgactg gatgccggta
     1141 aattacctgc cggtcaatac cgttacacag ccaaaaccga attcaatagt gcaaaatacg
     1201 aggttagcgg ggagttcagt attactccgg taatgcttga attagcaaaa tcagttgccg
     1261 atcaccaatt acttgctgca cttgctttcc aaaccaaagg cgaaatgatc catccaaatg
     1321 acattcgctc attaccggaa aagatcggga gccaggaaga tattgtcgag gttgtgtttc
     1381 ctgagcgccg attggatgat ctgttgcatt tcagatggat tttcttcctg atcctggccc
     1441 tgctcacttt ggagtggttt attcgcagat ggtcgggagc ctattgattt tccaattatg
     1501 aaatccagac tgattcttgc cttgctgttc ctcctgcttg atttttcagg atcagcacaa
     1561 tcattccgac tgcccgatag ttgggaacct caagatgtat tattggtcaa aacaaaaggg
     1621 agtcataccg gatctcaaac aacaatccgg tttcaatccg atcaatgtac ttatgaggaa
     1681 aatctggaag gtaagagcat tactattccc tttcaaatgc acccaaaaac atttgaagca
     1741 cttctatttt atcttaaaga cagaaagttt aatgaattga aattcaaaaa attaaaaact
     1801 atcacttacg acaaaaattc aagtatcatc tattggctaa gaggtggaaa gcagctcttc
     1861 cggattggtt atgatgcgga tattgaagtc caccggaact cagaaaaaag gttcaatatg
     1921 atcctgcttc acctgattca attagcagaa caacatactg aaaagctcaa agtaaaggtc
     1981 aattttacca ttcattcctc agtaaaagaa tccatgttgg acctcaacta ccgcatcgat
     2041 ggtagtggtc agcgtgcatg gtttggaaat agtcagatac caaacatgat cagtttaatg
     2101 ctgtttcggg taaggtacag ttttcacttt gctttgttgg acggtgcaaa gggactcaat
     2161 ggcgaaaacc cgggcacaat ctggacctta tttgaaccgg atattgccaa tcacaccgaa
     2221 tttgaaattc tgcttgaaag gaggagattg gttgttcgcc cactgtaaac tattaattct
     2281 gcagaatagc agagctaaat gttatcccaa tttttcttca acccagcttt ttgccaggtc
     2341 aaattgttct tcgggactca acattgtatt atcgatgatc cgggcatcaa tagcctgaac
     2401 cagtggattt tctttacggt gggtatcttc aaaatcgcga cttgaaatat tctcgattac
     2461 ctcgtccata ctcactttag cacctttagc ctttaattct tcatatcgcc ttcgcgctct
     2521 ggtttctttg tcggcagtca tgaaaatctt taattcagca tgcggaaaaa cggccgtacc
     2581 aatatcacga ccatccataa caacaccctt ttcctgcccc atggattgtt gcagggcgac
     2641 caattttact ctgacttctt tgatcaggct aactagtgaa acataccgag aaacttccat
     2701 tcctcggatt tcctgttcaa cgttttcatt gttaagaaag gtttcagacc gctgattttc
     2761 atgattgaac ctgaagcgaa tatcaatatc atccaaacac tcgatcaatt gatccttcat
     2821 cacgaaatta tcctttacca ttccctgacg aagacaaaaa agcgtaactg ccctgtacat
     2881 tgcacctgag tcaacataca caaatccaaa atgcctggcc agttgtttag ccatggtact
     2941 tttcccacaa gaggaatatc cgtcgattgc gatgttaatt tttttcaagc aagagcaaag
     3001 ataattcttc gcggcaatat cacctgaatt cgttcaggtt gaatgcaatt gtaatgtggt
     3061 tggataatcc cgcctgatta taggttgcaa gtgcataaga caaatggaat tttttgattt
     3121 tcattcccat tccaaacgaa aatccggata atccacctcg ctcacgtacc cttagttctg
     3181 ctcttctgcg gtaattaaaa ccgaaaccaa taaaaaagtt ttttgtcacc aactctgtcc
     3241 cgaatacagc gtgtcgcatc agcttatctc caaatcccgg tgctttgggt tctatttttt
     3301 cacctgtgga agggtcaata gtcggtttaa gcgttggatc taaatatgaa agatcccagc
     3361 gatg
//
LOCUS       DAOMYG010000360         3356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10287_length_3356_cov_6.874281, whole genome shotgun sequence.
ACCESSION   DAOMYG010000360 DAOMYG010000000
VERSION     DAOMYG010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10287_length_3356_cov_6.874281"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PK610_10945"
     CDS             <1..264
                     /locus_tag="PK610_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36498.1"
                     /translation="IVESCSSHVPADNYTSNETLSYYRAENSKYKLREEIRKRGYDPR
                     RLVFVETRALVQGPKYRRGDDTKSIVFENFQYIKIFPESMIKK"
     gene            complement(265..846)
                     /locus_tag="PK610_10950"
     CDS             complement(265..846)
                     /locus_tag="PK610_10950"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRJ36499.1"
                     /translation="MELLDFNKLNKLQPAKGHVLISEPFLDDDYFKRSVILLCEHNEE
                     GTFGFVLNNYLDIKLTDLIEDLPDLKTRVSIGGPVNSDNLFYIHTLGDKIPGSIPIFG
                     NVFMGGNWDVLRVALDSGSLKEEDIRFFVGYSGWSPGQLEEELSRSSWIVGKVEDSDV
                     MDQHSDDLWKRVLKGMGKNFSLIADYPEDPTMN"
     gene            979..1641
                     /locus_tag="PK610_10955"
     CDS             979..1641
                     /locus_tag="PK610_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HRJ36500.1"
                     /translation="MTIFKVLFLYWSAISLAAPQNASYQFAVLKYNGGGDWYANPTAL
                     PNLVEFCNKNLSMNISKEIASIEVGSPDLFNYPFVHMTGHGNVVFSQRDVDNLRKYLM
                     AGGFLHISDNYGMDPFIRKELEKLFPGSKLIELPPSHAIYNQKYAFPKGLPKIHEHDN
                     KPAQGFGLFYQQRMVLFYDYECDLGDGWEDPSVHKDSEETRTKALKMGANIVQYALMG
                     RD"
     gene            complement(1643..2638)
                     /locus_tag="PK610_10960"
     CDS             complement(1643..2638)
                     /locus_tag="PK610_10960"
                     /inference="COORDINATES: protein motif:HMM:NF014867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36501.1"
                     /translation="MSKSNAKSRSKSVQTSAEVITIVGCGKVGASIALLLLQQKKAGR
                     IINIMEPMAGRQGHIMELLHCQVMHPDTVIKINDTNCFSESNVIIHTAGIMQKIIGSR
                     MEVARGNIEMAVQIYRDVVFSNDPVMIVVSNPVDVISYFISQYAQIPSNRIIGTGTLI
                     DSFRMAANIAKYFGVNGSEVSAMVIGEHGETMVPVFSSVLVRNKPLGKQYLKDARFIQ
                     EIRTETIFAARNIKRTQSATYLGIADATVFLLDKLNSSAECILPASVMLPIGIKERLN
                     ISEDLFLSFPLKISGGNWTIQLPELEESEWSQLRVSAIYLEGVVNGLQGKSFAAE"
     gene            2706..3302
                     /locus_tag="PK610_10965"
     CDS             2706..3302
                     /locus_tag="PK610_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36502.1"
                     /translation="MIFLSLPNNRPGDIKTQEIRTTIETDEPKWLFDPDLGDGFSFPL
                     KGTYNAGILFYAGKNYSVQVKSENKEDQFIEWFILPNNFTYCQVHTMAKGRVIGIEHD
                     SLGFYYRIRHAFIENAKRCELDIPYGPVLISNLQPGQLIERQNPIGELAGKKLCVRVN
                     KTEKSPLFFNPPTWYAKEYRNTTFPKTEENIIVVRKKD"
BASE COUNT         1023 a          730 c          643 g          960 t
ORIGIN      
        1 attgtagaat cctgttcttc ccacgttcct gctgataatt ataccagcaa tgaaacctta
       61 tcctactacc gggcagaaaa ttcaaaatat aagctgagag aagagatcag gaaaagaggc
      121 tatgatccga ggcgactcgt ttttgtggaa accagagcac ttgtgcaagg tccgaaatac
      181 aggcgcggtg atgacaccaa gagcattgta tttgagaact ttcaatacat taagatcttt
      241 ccggaaagta tgattaaaaa atgatcagtt cattgtagga tcttcaggat aatcggcaat
      301 aagtgagaaa ttcttaccca tcccttttag tactctcttc cagagatcat cagaatgctg
      361 atccattaca tcggaatcct ccacttttcc aacaatccat gaactcctgg aaagttcttc
      421 ttccaactgt ccgggtgacc accctgagta accaacaaaa aaacgaatat cttcttcctt
      481 taaggatcct gaatccagtg caacgcgcaa aacatcccaa tttcctccca taaaaacgtt
      541 accgaaaatt ggaatactgc ccggtatttt gtcgccaagc gtatgaatgt aaaataaatt
      601 atcactgtta accggtccgc ctatactcac ccttgttttt aaatcgggca aatcttcaat
      661 gagatctgtt aatttaatat cgagataatt attgagcaca aaaccaaagg tgccttcctc
      721 attgtgttcg caaagcaata tcactgagcg cttaaaataa tcatcgtcca gaaagggctc
      781 cgaaataaga acatgtccct tagccggttg tagtttattc agtttgttga aatcaagtaa
      841 ttccatcttt attttctcct tcgctgttct aagtccttca acggccggaa gcattatttg
      901 ttgccggaga ttatgaacaa cggttaaaat ccaatatact acaaagcatc gaaaacgaaa
      961 aattcggata tttaggccat gacaattttt aaagtcctgt ttctttattg gtctgcgatc
     1021 agcttggctg cacctcagaa cgcgtcctat cagttcgctg ttttgaaata caatggcggt
     1081 ggcgattggt atgccaaccc tacagccctt ccaaatctgg tggagttctg taacaagaat
     1141 ttatccatga acatctccaa agaaatagca agcattgaag tgggcagtcc tgatctgttc
     1201 aattatccgt ttgtccatat gaccggtcat ggcaatgttg tgttctctca gcgcgatgtt
     1261 gacaatcttc gtaagtattt aatggccggt ggttttttgc atatctccga taactatgga
     1321 atggatcctt tcatccgaaa agaactggag aaactatttc ccggtagtaa actgattgag
     1381 ttgccacctt cccatgccat ttacaatcag aaatacgctt ttccaaaagg actccccaaa
     1441 atacacgagc acgataataa acctgcccag ggattcggac tattttacca gcaaagaatg
     1501 gtgctttttt acgactacga atgcgacctg ggggacggat gggaagaccc gagtgtgcat
     1561 aaagattcgg aagaaacacg cactaaagcc cttaaaatgg gtgccaacat tgtgcaatat
     1621 gcattaatgg gccgggatta aactattcgg ccgcaaacga ttttccctgc aggccattga
     1681 caacaccttc caaataaatt gccgacacac gcaattgaga ccattcactt tcttccaatt
     1741 caggtaactg aatggtccaa tttccaccgg aaatctttaa gggaaaactt aagaataaat
     1801 cctcggaaat atttaagcgt tcctttattc caattggtaa catcaccgag gcgggtaaaa
     1861 tgcattctgc tgaagaattc aatttatcca acagaaaaac tgttgcatca gcaattccca
     1921 aataagtagc gctttgtgta cgttttatat ttcgggctgc aaaaatagtt tcagttcgaa
     1981 tttcctgaat aaaacgtgca tctttcaagt attgcttccc aaggggtttg ttacgcacca
     2041 gcacactcga aaataccggc accatcgttt cgccatgttc accaatcacc attgcactga
     2101 cctcagagcc attaactcca aagtatttgg caatattggc agccattcgg aatgaatcaa
     2161 taagagtacc tgtaccaata attcggttag aaggaatttg ggcgtattgc gaaatgaaat
     2221 aactgattac atcaaccgga ttggaaacca caatcataac cggatcattg ctgaacacca
     2281 catcgcgata aatctggaca gccatttcaa tatttccacg cgctacttcc attctggaac
     2341 cgatgatctt ttgcatgatt ccggctgtgt gaatgataac attcgattca gaaaaacagt
     2401 ttgtatcgtt tatttttatt acggtatcag gatgcataac ctgacagtgc agcaactcca
     2461 taatatgccc ctgacggccg gccatgggct ccatgatatt gataattctt ccggcttttt
     2521 tttgttggag caacagcaaa gcaatagaag ctcccacctt gccacaaccc actattgtta
     2581 taacctctgc agaagtttgc acactttttg accttgactt tgcgttactt ttactcacac
     2641 tgcgaatttc acaaatgaaa aaaaagtgat taatgactta catcagtatg ccaattttta
     2701 ttttgatgat atttcttagt cttcccaaca accggcccgg ggatattaag acgcaggaaa
     2761 tcagaaccac cattgagact gatgagccga aatggttgtt tgatcctgac cttggagatg
     2821 gattttcatt tccgttaaaa ggaacttaca atgcaggaat tttattttat gcaggaaaaa
     2881 attattcagt tcaggtaaag tccgaaaaca aggaagatca attcattgaa tggtttatcc
     2941 ttcccaataa ttttacctac tgccaggttc atacaatggc aaaaggtcgg gtcattggga
     3001 ttgaacacga tagtttagga ttctattaca ggatccgtca tgcctttatc gaaaatgcaa
     3061 aaagatgtga actggatatt ccttacggtc ctgtgctcat cagtaatttg caacccgggc
     3121 aattgatcga acggcagaat cctatcggtg aattggcggg gaaaaaactt tgtgtccggg
     3181 ttaataaaac agagaagtcc cctcttttct ttaatccccc aacctggtat gcaaaagaat
     3241 acaggaatac cacctttcct aaaactgagg agaatattat agtggtgcgc aaaaaggatt
     3301 agtccctttt tcattaccgg aagggggagt taaattcacg tttcgatatt tgtctg
//
LOCUS       DAOMYG010000361         3351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10311_length_3351_cov_9.201153, whole genome shotgun sequence.
ACCESSION   DAOMYG010000361 DAOMYG010000000
VERSION     DAOMYG010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10311_length_3351_cov_9.201153"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PK610_10970"
     CDS             complement(<1..1115)
                     /locus_tag="PK610_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HRJ36503.1"
                     /translation="MSATKEKVSVKSHILIEGARVHNLKNVSVAIPRNKLVVVTGLSG
                     SGKSSLAFDTLYAEGQRRYVESLSAYARQFLGRLDKPEVDHIRGISPAVAIEQKVISR
                     NPRSTVGTVTEIYDYMKLLFARVGKTYSPVSGKEVKRERTADVVNYIQQLSKGTRVIL
                     FAPFIRKNDRTHQKQLELLQQQGFTRIELNGQILTIEDALSKKFKNADKIYVMIDRFV
                     AEAGNEENESRIADSVETAFFEGEGECVVEVQDEKKTRRFFSNKFEADGVLFEEPSVH
                     FFTFNNPIGACKTCEGFGSVIGIDPDLVLPNKSLSVFEDAVACWKGETMGEWKKQLIK
                     TADKFNFPIHKPVGQLTKEQMNVLWKGNKHFDGLDEF"
     gene            1404..>3351
                     /locus_tag="PK610_10975"
     CDS             1404..>3351
                     /locus_tag="PK610_10975"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ36504.1"
                     /translation="MHRSRIAFLVVSFFQLLSIPSFSQEKFTVSGYVKDGATGEYMMA
                     ANVYVIETQKGTTTNGYGFYSITLPKGKYKLKFSFIGYQDLTKDVELNGNTTLNVQLN
                     PDSYQIDEIEVVADGAKENTNSTDMGRVGLDVEKIKTLPAFMGEVDIMKTIQLLPGVQ
                     AAGEGNSGFYVRGGGPDQNLILLDEAVVYNASHLFGFFSVFNADAVRNVELYKGSMPA
                     NFGGRLASVVEVSMKEGNSKEIKVDGGIGLISSRLTVQGPIKKDTSSFMISGRRTYID
                     VLMKPFIKESSPFRGTGYYFYDLNAKFNYRFNDKDRLFFSGYFGKDVFDFGNKDAGFG
                     VRIPWGNATATVRWNHLFNSKLFMNVSAIYSKYDFQFAASQDDFEFAIFSGIQDWNGK
                     LDFFYLPNPRHNIKFGAFYTYHTFIPSSATARQGDLEFDTGDRVKLFAHEAAIYFNDD
                     FDLTEEIRISAGLRYSAFNQVGPFKRYVKDPTLGTTIDTIQYSSGESVKLYHGPEPRL
                     SMRYTLNKVSSLKAAYTRNLQFIHLASLSAVSLPTDVWVPSTDRVNPQIGNQYSIGYF
                     RNLKKDAYETSVEVYYKTMQNQVEYREGAQPDDDVKDNPDNNFVFGYGWAYGAEFFLK
                     KRSGKFNGWVGYTLSWTKRRFDDLN"
BASE COUNT          912 a          681 c          719 g         1039 t
ORIGIN      
        1 aaaaattcat ccagtccgtc gaaatgttta tttcccttcc aaagtacatt catttgttct
       61 ttggttagtt gtccgaccgg tttgtgaata gggaaattga atttatctgc agttttgatc
      121 agttgttttt tccattctcc catcgtttcg cctttccagc aggctactgc atcctcgaaa
      181 acggaaagcg atttgttggg aagcaccaga tcaggatcaa taccaattac acttccaaac
      241 ccttcgcagg ttttgcaggc tccaatggga ttgttaaacg taaaaaagtg gacactgggt
      301 tcctcaaaca aaaccccatc cgcttcaaac ttattgctga agaatctgcg ggttttcttt
      361 tcatcctgca cttcaaccac acattctcct tcaccttcaa aaaatgcagt ttcaacagaa
      421 tcggcaatgc gactttcgtt ttcttcgtta cctgcttctg caacaaaacg gtcgatcatc
      481 acataaatct tatcggcatt tttaaatttc ttggaaagag catcttcaat agtgaggatt
      541 tgtccgttaa gctctattct ggtaaatcct tgttgttgga gtaattcaag ctgtttttgg
      601 tgtgttctgt catttttgcg aatgaaggga gcaaacaaaa tcacacgtgt ccccttgctg
      661 agttgctgaa tgtaattgac tacatccgct gttcgttcgc gcttgacttc ctttccgcta
      721 accggcgaat aggtttttcc aacgcgtgca aaaaggagtt tcatgtaatc gtagatctcc
      781 gtaactgtac caacagtaga acggggattt ctgctgatta ccttttgttc aattgctaca
      841 gcaggtgaaa ttcctctaat gtgatcaact tcgggtttgt cgagcctgcc cagaaactgt
      901 cgtgcgtatg cagaaaggct ctcaacatac cttcgctgac cttctgcgta aagggtgtca
      961 aaagccaacg atgatttgcc cgaaccggat aaacccgtta ccacaaccag tttgttgcgg
     1021 ggaatggcaa cactcacatt tttcaggttg tgtacgcgag ctccttcaat gaggatatgt
     1081 gacttaacgg agactttttc tttggtggct gacatagggt actttcggga aagctgcaaa
     1141 attagcaggt atttgggtta tttaccttaa aaaagccatt aggatgagta ctgtggacaa
     1201 ctttttctgt gctgctgtat ggtcatatca ggaaatggct tgttttttcg cttttgttga
     1261 ccgtcttctt tagaaaattc gtaatgaaca tttaaccttt ttgatgtttc ttttcacctt
     1321 taacaaacaa ttcatccgat aatttgtttt ttccttgggt acttgtcatt ttttaataag
     1381 ttttgcaccc gaatcctgaa accatgcacc gtagtcgaat tgcatttctc gtcgtatcgt
     1441 ttttccagct tctatcaatt ccgtcatttt cgcaggagaa attcactgtt tccggatacg
     1501 ttaaagacgg tgctaccggc gaatacatga tggctgccaa tgtgtacgtt atcgaaaccc
     1561 aaaagggtac tacaaccaac ggatatggtt tttactcgat cacgcttcct aaaggaaagt
     1621 acaaactcaa attttcattt atcggttacc aggatctgac aaaagatgtc gagctcaacg
     1681 gaaacacaac gcttaatgtc cagttgaatc cggattcgta ccagattgat gagattgaag
     1741 ttgttgccga tggagcaaag gagaacacga acagtaccga tatgggtagg gttggattgg
     1801 atgtggaaaa aattaaaaca cttccggctt tcatgggaga agtagacatc atgaaaacga
     1861 ttcaattgct tccgggtgta caagcagccg gcgaaggaaa ttcgggtttc tatgttcgag
     1921 gtggtggtcc tgaccaaaac ctgattcttc tggatgaagc agtggtgtac aatgcatccc
     1981 acctgttcgg attcttttct gtttttaatg cggatgcagt tcgcaatgta gagttgtaca
     2041 aaggttccat gcccgcaaat ttcgggggac gccttgcctc ggtggtggag gtttccatga
     2101 aagaaggcaa tagtaaggaa ataaaagtag atggtggaat cggacttatt tcatcacgac
     2161 ttactgttca gggtccaata aaaaaagaca ccagctcatt tatgatttca ggtcgcagaa
     2221 cctatattga tgtgctcatg aaaccattca ttaaagagag ctctcctttc agaggtacag
     2281 gatattattt ttatgatttg aatgccaagt ttaattaccg tttcaacgat aaggacaggt
     2341 tgtttttcag tggttatttt gggaaggatg tgtttgattt cggaaataaa gatgccggtt
     2401 ttggggttcg tattccttgg ggaaatgcga ctgcaacggt tcgatggaac cacctcttca
     2461 attcgaaatt gtttatgaat gtttccgcta tttacagcaa atacgatttt cagtttgccg
     2521 caagtcagga tgattttgaa ttcgcaattt tttccggtat tcaggattgg aatggaaagc
     2581 tggatttttt ctacttaccc aatccgcgac acaatatcaa gtttggagcg ttttatacct
     2641 accacacctt cattccttcc tctgcaacag cacgtcaggg agatcttgaa ttcgataccg
     2701 gagatcgtgt aaaattgttt gcccacgaag ctgcaattta ttttaacgat gatttcgatc
     2761 ttactgagga gatcagaatc agtgcaggct tacgttattc cgcatttaat caggttggac
     2821 catttaagcg ttatgttaag gatccaacac tgggaacaac catcgataca atacagtatt
     2881 cttccggcga aagtgttaag ctgtaccacg gtccggaacc gagattatcg atgcgctata
     2941 cattgaataa agtttcttca cttaaggctg catatacccg gaatcttcaa ttcattcacc
     3001 ttgcatcttt atcagcagtg tcacttccaa cagatgtttg ggttccatct accgatcgtg
     3061 tcaatcccca gatcggaaac cagtacagta tcggttattt caggaatttg aaaaaggatg
     3121 cctatgaaac ttcagtggaa gtgtattata aaactatgca aaaccaagtg gaataccgcg
     3181 aaggtgcaca gccggatgat gatgtaaagg ataatcccga taataatttt gtatttggtt
     3241 acggttgggc ttacggtgca gaattttttc tgaaaaagcg ctccggaaaa ttcaacggtt
     3301 gggtgggata taccttaagc tggaccaaac ggagatttga tgatctcaat a
//
LOCUS       DAOMYG010000362         3342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10365_length_3342_cov_9.324308, whole genome shotgun sequence.
ACCESSION   DAOMYG010000362 DAOMYG010000000
VERSION     DAOMYG010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10365_length_3342_cov_9.324308"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..2113
                     /locus_tag="PK610_10980"
     CDS             38..2113
                     /locus_tag="PK610_10980"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HRJ36505.1"
                     /translation="MKKVFLIMALVWPFISHSQSSFPCGSDHMRRVRIAQDSSILIKE
                     HLLEQFTQQFVNQESSNRTNQVYTIPLVFHILHQKGPENISDAQIHDAVAILNRDFRK
                     LNADTSQIVAGFDTLAEDAKIEFRLATIDPLGNCTNGIDRIYSPETYVGDDGSKLNQW
                     PRNKYLNIWVVSQMENGVAGYAYYPSAVDAGLMAMVDGVIILHNYIGSIGTSNPYRSR
                     ALTHEVGHWMNLQHPWGNNNDPGVACGDDQVNDTPITKGWTTCNLGGNVCNPGIVENV
                     QNYMDYSYCSLMFTKGQVLRMQAALESTEAGRNNLWSAQNLLATGVDPSTFQVCAPKA
                     DFYPDRQIVCLGDAVQFIDNSTRAEATTWMWNFQDGNPATSNLQSPLVNFTSPGWKQV
                     SLNVSNSEGNDTKTINRSVYISAGIPDYSMPYWESFEWYLPFNEKWIVNNLGENSNAF
                     SLYQGVGYTGNYCIKLGYQNPLPIPFFDGDKDIDELITPSFNLSGSLGTNFNFRYSYA
                     TNALQLSNITEKLQVYSSVDCGNTWFLRKTITGTDLASGFGSSGASFMPTSPSQWGYD
                     EIYISGSLAQPDVRFKFVFISSPYSNNLYLDDINIGTSTEVPEFRLNDIDVFPNPVND
                     FLTIKFNKILPKDLLIELFDITGKKLERITFISESDIFSLDFSQYPSGLYLVRLSFGE
                     QSRCIQIIK"
     gene            2260..3066
                     /locus_tag="PK610_10985"
     CDS             2260..3066
                     /locus_tag="PK610_10985"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRJ36506.1"
                     /translation="MKSVIEKAWENRELLKEKETQDTIRAVIEEIDKGRLRVAEPSGN
                     EWVVNEWVKKAVVMYFPIQQMEVIEVGPFEFHDKMKLKTGYKELGVRVVPHAVARYGA
                     FVAPGVIMMPSYVNIGAYVDSGTMVDTWATVGSCAQIGKDVHLSGGVGIGGVLEPLQA
                     APVIVEDGCFVGSRCIVVEGVRVKKQAVLGANVVLTKSTKIIDVTGATPIEYKGEVPE
                     RSVVIPGSYTKKFPAGEYQVPCALIIGTRKESTDLKTSLNDALREYNVAV"
BASE COUNT          976 a          677 c          738 g          951 t
ORIGIN      
        1 tatacaaact caaaatttat ttccaaaatt aatgtacatg aaaaaagttt ttctcataat
       61 ggcattggta tggcctttta ttagccattc gcaatcctct tttccatgcg gatcagacca
      121 tatgcgacgt gtcagaattg ctcaggattc ttcgatttta ataaaagaac atctactcga
      181 gcaatttacg caacaattcg tcaaccaaga atcatctaac cgaacgaatc aggtgtacac
      241 gattccttta gtgtttcata ttcttcatca aaaaggaccg gaaaatattt cagatgctca
      301 aatacatgat gcggttgcaa ttctaaatcg tgatttccgc aaattgaatg cagacacgtc
      361 gcagattgta gcaggatttg acacactggc tgaagatgca aaaatcgaat tccggcttgc
      421 cacaattgat ccactaggaa attgtacaaa cggaatcgac cgaatctatt ctccggaaac
      481 gtatgtcgga gacgatggat caaaactcaa tcagtggccc cgcaataaat accttaacat
      541 ttgggtggtt tctcaaatgg aaaatggggt tgccggttat gcctattatc cttcagctgt
      601 agatgccgga ttaatggcga tggtggatgg cgtaatcatt ttgcacaatt atattggctc
      661 aattggaaca agtaatcctt atcgttcacg tgcacttaca catgaagtcg gccattggat
      721 gaacttacag catccctggg gaaataataa cgatccaggc gtagcctgtg gcgacgatca
      781 agttaatgat acaccgataa caaaaggttg gacgacctgc aacctgggtg gaaatgtttg
      841 caatcctgga atcgtagaaa acgttcagaa ctatatggac tactcctatt gctcgctaat
      901 gtttacaaaa ggtcaggttc tccgaatgca agcagcactt gagtccaccg aggctggaag
      961 aaataattta tggtctgccc aaaatcttct ggcaactgga gttgatccct caacatttca
     1021 ggtttgtgca ccaaaggctg atttttatcc ggaccggcaa attgtttgtc ttggtgatgc
     1081 ggtacagttc atcgacaatt ccactcgcgc tgaagcgaca acatggatgt ggaattttca
     1141 ggatggtaat cctgccactt cgaatttgca gtctcccctg gtaaatttta cttcaccagg
     1201 ctggaagcag gtttcactca acgtatctaa tagcgaaggg aatgatacca agacgataaa
     1261 cagatccgta tatatatctg cggggattcc agattattct atgccctatt gggaaagttt
     1321 cgaatggtat ttacccttca acgaaaaatg gattgtaaat aatctaggcg aaaactcaaa
     1381 tgcgttctcc ttgtatcaag gtgttggata tacggggaat tattgtatca agctaggtta
     1441 ccaaaatcct cttcctattc cgttttttga cggagataaa gacatcgatg aattaattac
     1501 cccctcattt aacttaagcg gatctttagg aacaaatttt aactttaggt attcttacgc
     1561 aacaaatgcc ttacaactat caaacattac cgaaaaactt caggtctatt catcagtcga
     1621 ttgcggtaat acatggttcc taagaaaaac aataacaggt actgatctcg catcaggttt
     1681 tggtagcagt ggtgccagct ttatgccaac atcaccttct caatggggat atgatgaaat
     1741 ctatatctca ggatccctgg cgcagcctga tgttcggttt aaatttgtat ttatcagttc
     1801 tccctattct aacaatttgt atttagacga tattaatatc ggcaccagta cggaagttcc
     1861 cgagtttagg ctgaacgata ttgatgtgtt tcccaaccca gttaatgatt tcctcaccat
     1921 taagttcaat aaaattctcc caaaagactt attgattgaa ctctttgata ttaccggaaa
     1981 gaaactggaa agaattacat tcatatctga atcagatata ttctccctgg attttagtca
     2041 gtatccgtca ggcttgtatc tagttcgtct ttctttcggt gagcaaagca gatgtattca
     2101 gatcatcaaa taattcaaaa attgttaatg cacagaaaag cctctttggg aaaccaaagg
     2161 ggcttttttt tgagcagaaa ctttcaaaaa caaaacagat tcatattcaa ttgcattatg
     2221 tattatattt atccagaaaa aactgactat ttcaatagta tgaaatctgt tatagaaaaa
     2281 gcctgggaga acagggaact gctcaaagaa aaagaaacac aagacaccat tcgtgcggtg
     2341 atcgaagaga tcgacaaggg acgtctgcgc gttgcagaac cttccggtaa cgaatgggtg
     2401 gtgaacgagt gggtgaagaa agccgtagtg atgtattttc ccattcagca aatggaagtg
     2461 attgaagtgg gtccattcga gttccacgat aaaatgaaac tgaaaaccgg ttacaaggaa
     2521 cttggcgtac gtgttgttcc gcatgctgtt gcgcgttatg gtgcattcgt tgctccggga
     2581 gtaatcatga tgccatcata cgtaaacatc ggtgcctatg tagatagcgg aaccatggtg
     2641 gatacctggg ctacggtggg tagttgtgcg cagatcggaa aagatgtaca cctctcgggc
     2701 ggtgttggta ttggcggtgt actcgaaccc ttgcaggctg ctccagtgat tgtggaggac
     2761 ggttgctttg tcggatcgcg ttgcattgtg gtagaaggtg tgcgtgtaaa aaaacaagcc
     2821 gtattgggtg caaatgtagt gctgaccaaa tccaccaaaa ttattgacgt taccggtgca
     2881 acaccgatcg aatacaaagg cgaagtgcct gaacgttcgg tggtgattcc gggttcctac
     2941 accaaaaaat tccccgccgg cgaataccag gtgccctgtg cattgattat tggaacgcgc
     3001 aaggaaagca cggatctgaa aacttcgctc aatgatgcat tgagggagta caatgtggcg
     3061 gtgtgaggaa tgtgggaatg tgctaatgtg ggaatccttc ttcgcttaag ctacgaagga
     3121 caagtgtgcg aatgtgctag tcctgacgct cctttcagtc ggtcgggatg attctccgat
     3181 ctgtcgaatc atgtgctaat acgccttcgc tgaagctatg tcaaacgatt gtgcccccct
     3241 acaccccgta tagttaattt cgcttttgta taaattggcc ccttggcaat tggaaattgg
     3301 ttcattggaa attggcatct ttcctcgtct caattggtat ac
//
LOCUS       DAOMYG010000363         3340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10378_length_3340_cov_8.631050, whole genome shotgun sequence.
ACCESSION   DAOMYG010000363 DAOMYG010000000
VERSION     DAOMYG010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10378_length_3340_cov_8.631050"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PK610_10990"
     CDS             complement(<1..907)
                     /locus_tag="PK610_10990"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRJ36507.1"
                     /translation="MNFIGIIEQLMKSPAIHKGNRDRAILEILVKSAQGLQVERVNVW
                     TFDKQKTTLSCIGSYESSTGEFNKGAVLLLKDYPVYFEHVRTAEKVISDDALASSFNK
                     ELLDSYILPLDIRSMMDIPIRSEGEMIGMVCFEQVGEKHLWTVSEQSFALSIAQLFSL
                     VFETHEKNEFRRQLEEALHEKEVLLSEVNHRVKNNMMIIGSLVNLQKYKAKDEYHVGL
                     FDEVRERITSMAYVHEQLYLSRNYSEIDFGLYLEKLLNHLLSSMRHGRNIRIETHLEH
                     IPIDIQRAIPCGLITNELVTNSFKYA"
     gene            complement(1013..2302)
                     /locus_tag="PK610_10995"
     CDS             complement(1013..2302)
                     /locus_tag="PK610_10995"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HRJ36508.1"
                     /translation="MSNTAKIILDGQEYELPVIEGTEGEKAIDISKLRDQTGYITLDT
                     GYKNTGATKSGITFLDGENGILHYRGYSIEQLAEKSNFIEVAYLLIFGELPTAAQLKK
                     FNDDITMHTLVHEDMKRFYEAYPTGAHPMGILASLMSSLSTFYPNALDPNRNEERVML
                     TIYRALAKMPTLATWAYKNSMGHPVVYPQNKYSYTENFLHMMYSMPSMDYTPDPVVAR
                     ALDVLLILHADHEQNCSTSTVRIVGSSQANLYSAISAGIAALWGPLHGGANQEVIEML
                     EAIKNDGGNVDKWVAKAKDKNDPFRLMGFGHRVYKNFDPRARIIKKACDDVLNKMGIH
                     DPVLEIAKKLEEVALNDPYFKERNLYPNVDFYSGIIYRALGIPVDMFTVMFAIGRLPG
                     WISQWKEMRENKEPIGRPRQIYTGYTSREYVDISKRK"
     gene            2521..>3340
                     /gene="guaA"
                     /locus_tag="PK610_11000"
     CDS             2521..>3340
                     /gene="guaA"
                     /locus_tag="PK610_11000"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRJ36509.1"
                     /translation="MHEMILILDFGSQYTQLIARRVREMNVYCEIHPYNKVPHTEHTI
                     KGLILSGSPFSVHDPGHPIPDLSPYLDKVPVLGVCYGAQFLAQHDGGLVERSKIREYG
                     RANLKYVDDNSALTKGMSEHSQVWMSHGDTITRVPEGCEVIASTADVNVAGYQYKGRN
                     IYGIQFHPEVYHSTEGAVLLGNFVKGICGLKGDWTPDSFIEATVAELKSKVGNDVVIM
                     GLSGGVDSSVAAILLHKAIGKNLHCIFVDNGLLRKNEFEEVLESYKHMGLNVTGV"
BASE COUNT          862 a          722 c          782 g          974 t
ORIGIN      
        1 atgcatattt aaaggaattg gtgaccagct cattggtaat taacccacat ggaatagccc
       61 gttggatatc gatgggaata tgctccagat gtgtttcaat tcgaatattt cttccgtgcc
      121 gcattgagct gagcaggtga ttgagtagtt tttcgagata taagccaaaa tcgatctccg
      181 aataattgcg cgagaggtaa agttgttcat gaacgtaagc cattgatgtt atgcgctctc
      241 ttacttcatc aaacaagcct acatggtatt cgtcttttgc tttgtacttc tgcaggttga
      301 cgagactacc aatgatcatc atgttgtttt tcactctgtg gttgacctca ctcaataaca
      361 cttctttttc atgaagcgct tcttccaatt gacggcgaaa ttcatttttt tcatgcgttt
      421 caaaaaccag ggaaaacaac tgggcaatac tcaatgcgaa actttgttcc gataccgtcc
      481 acaaatgttt ttcgccgact tgttcaaaac ataccattcc gatcatttct ccttcactgc
      541 gaatcggaat atccatcatg ctccgtatgt cgagcggtaa aatgtatgag tcaagtaatt
      601 ctttattgaa cgaactggca agtgcatcat cactgatgac tttctcagcg gttcgcacat
      661 gctcaaaata caccggataa tctttcagca acaataccgc acccttattg aattcaccgg
      721 tgctggattc gtaggaacca atacaactca atgtggtttt ttgtttatcg aaggtccaga
      781 cattcacacg ttccacctgc agaccctgtg cgctttttac cagaatttca agaattgccc
      841 gatccctgtt gcctttgtgg atggcgggac tcttcatcaa ttgttctatg attccgatga
      901 aattcatatt ctgctaacag aaatttaagc ggatatcctg tcaaatgcaa gcctgtcgac
      961 gaatattcgt ccaaaaaaaa aatcccgcat ctgcgggatt ttttctgttg tgttattttc
     1021 ttttcgaaat atccacgtat tcgcggctgg tgtagccggt gtagatctga cgcggacgac
     1081 cgattggttc tttgttttcg cgcatttctt tccactgaga aatccaaccc ggaagacgtc
     1141 cgatagcgaa catcacggtg aacatatcaa cagggatacc gagtgcacgg tatatgattc
     1201 cggaatagaa gtcaacgttc ggataaagat tacgctcttt gaagtaagga tcgttaagag
     1261 ccacttcttc aagttttttg gcaatctcaa gaacgggatc gtgaattccc atcttattca
     1321 atacatcgtc gcaagctttt ttgatgatac gtgcgcgtgg atcgaagttt ttgtacacac
     1381 ggtgaccaaa gcccatcaaa cggaagggat cgtttttgtc tttggcttta gcaacccatt
     1441 tgtcaacgtt tccaccgtcg ttcttaatgg cttcgagcat ttcaatcact tcctggtttg
     1501 caccaccgtg tagtggtccc cacaacgctg caatacctgc agaaattgca gaatagaggt
     1561 tggcttgtga tgaaccaacg atacgcactg tagaggtcga acagttttgt tcgtggtcgg
     1621 cgtgaaggat aagcaataca tcaagcgcac gtgcaacaac cggatcagga gtgtagtcca
     1681 tgcttggcat agaatacatc atgtgcagga agttttcggt gtacgaatat ttgttttgcg
     1741 gataaaccac cggatgaccc attgagtttt tgtatgccca ggtagcgagc gtaggcattt
     1801 ttgccagtgc acggtaaatg gttagcatta ctcgctcttc attgcggttg ggatcaagcg
     1861 cattggggta gaatgtagac aacgatgaca tcagcgatgc aagaattccc attgggtgag
     1921 cgcctgtagg gtatgcttcg taaaaacgtt tcatgtcttc atgcaccagc gtatgcatgg
     1981 ttatatcgtc attgaatttc ttcaattgag cggcagtggg aagttcaccg aaaataagca
     2041 ggtaagctac ttcaatgaaa ttggatttct cggcaagctg ttcgatggaa tagccacggt
     2101 aatgaagaat tccgttctct ccgtcgagga aagtgatgcc acttttggta gctccggtgt
     2161 ttttatatcc ggtatcaagg gtgatgtacc cggtctgatc acgaagtttg gagatgtcaa
     2221 ttgccttttc tccttcggtg ccttcaatga caggtaactc gtactcctgt ccgtcgagaa
     2281 tgattttcgc tgtgttgctc atgtatcggt gatttatgtg tggaaacggt tttaaaaata
     2341 atacaaagtg tgatatggaa cagggtaaga tgaaaaaaaa gtcggaatcg ctgaaagtat
     2401 ccgccaaggc cccgtttttg ggcgacggat tttggtcgga tcggtcggca ggttcccgat
     2461 tgttgacaat ttcaccgttt tacacagttc tgcggcagtg gactttttat ctttgccccc
     2521 atgcacgaaa tgatccttat tctcgatttc ggatctcagt acacgcagct cattgcccgg
     2581 cgcgttcggg aaatgaatgt atactgtgag attcatcctt acaacaaggt accccatacc
     2641 gaacatacca taaaagggtt gatcctttcc ggaagccctt tctccgttca tgatccgggc
     2701 caccccattc ctgatctttc tccttacctg gataaggttc ccgttcttgg cgtgtgctac
     2761 ggtgcccagt ttctggctca gcacgatggg ggactggtag aacgttccaa aatcagggag
     2821 tacggacgtg ccaatctgaa gtatgtggac gataattctg ccctgaccaa agggatgagc
     2881 gaacactcac aggtatggat gtcgcacggg gataccatta cacgtgttcc cgaaggatgc
     2941 gaagtgattg cttcaacagc cgatgtgaat gttgcagggt atcagtataa aggacgaaac
     3001 atctacggaa ttcaattcca cccggaagtg taccattcta cagaaggtgc tgttttattg
     3061 gggaattttg taaagggaat ttgcggatta aaaggcgact ggactcccga ttcttttatt
     3121 gaagctaccg ttgcagagtt gaaatccaaa gtggggaatg atgtggtgat catggggctt
     3181 tcaggcggag tggattcgtc ggttgctgcc attctcctgc acaaggccat cggaaaaaat
     3241 ctgcattgca tttttgtgga taacggacta ctccgtaaga atgaatttga agaagtactg
     3301 gaaagctata agcacatggg actcaacgtt accggtgtgg
//
LOCUS       DAOMYG010000364         3339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10385_length_3339_cov_5.382460, whole genome shotgun sequence.
ACCESSION   DAOMYG010000364 DAOMYG010000000
VERSION     DAOMYG010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10385_length_3339_cov_5.382460"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="PK610_11005"
     CDS             <1..865
                     /locus_tag="PK610_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HRJ36510.1"
                     /translation="HIEIQVLADTHGNVVYLFERECSIQRRHQKLVEEAPSAILTPEI
                     RSAMGKCAVDICLACNYRGAGTVEFLVDEQLNFYFLEMNTRLQVEHPVTELITGIDLV
                     KEQIKVARGEKLSFSQSDLKIHGHALEVRVCAEDPDNNFLPEMGKLITYRPPEGPGVR
                     VDDGYEEGMEIPIYYDPMISKLITYGKDRTEAIERMVRAISEYEISGVETTLKFCAFA
                     IQHDAFVSGHFDTNFVKNYFVPGSLHKEMTDEMEVAAIFASGHSSSKPKIQANSGNSA
                     ESLWKKRKELR"
     gene            910..1635
                     /locus_tag="PK610_11010"
     CDS             910..1635
                     /locus_tag="PK610_11010"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HRJ36511.1"
                     /translation="MLPKGTYIILILLLSFLRVNGQNPDSLSRFRPGYVNSVVVNNDT
                     LHQYFLYDVTITASDSVYKAKYTRFLRYVRKTYPYVMLAKQLVAEYEKDLAEKNSKRA
                     EKKYINQEFEELKDRFTDGIAVLSVNEGKVLVKLIHRETGMTAYEIAHKYLGTARALI
                     WQGISRTGGANLKLTFNPDEVDDKILEGIVKKVEKGEIWVPKEPFVPANRKQYEKERR
                     QKSREYKQEQKKKSSAASTSSVK"
     gene            1688..2080
                     /locus_tag="PK610_11015"
     CDS             1688..2080
                     /locus_tag="PK610_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36512.1"
                     /translation="MYKAGLLLVFISLFSVSATQAQNQKVSCDSVYTYAENPPQYPGG
                     MKAFYDFLKGFHYQDVGPNDPVVGKFNIHLTLDAEGKPIDVQVTPESDPGLALKEYLI
                     KMQTWTPASTKDGKVCYFLKIPAYVHYK"
     gene            2005..2826
                     /locus_tag="PK610_11020"
     CDS             2005..2826
                     /locus_tag="PK610_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRJ36513.1"
                     /translation="MDSRFNKGWESVLFFKNTRLRPLQVITTAEIKSVKSLHQKKFRE
                     ESGLFLVEGEKLLHELLHSEWEIERICATSSWLDKNKINQGIIINEVKSSELERISAL
                     TQPNEVLAVVRQRKFDFNLKTAQNGLTILLDDVRDPGNLGTIIRIADWFGVRQIVCSP
                     QSVELFNPKTIQSTMGSAFRIPVVYTDLLPLVHDLKKEGIAICGAVMEGKNVLSFEWK
                     KSTALVMGSESHGINARLSALLDEHVTIPRFGEAESLNVSVATGIFCAMFNRQFS"
     gene            2879..>3339
                     /locus_tag="PK610_11025"
     CDS             2879..>3339
                     /locus_tag="PK610_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36514.1"
                     /translation="MKEYGELFLNNFFGYFNYLKDEILFKTEPWYHNYFYWLILISVF
                     FWLLEIIAPWRQRQAVIRKDFWLDGFYMFFNFFLFSLIGYAAISYVFVELFNDFLGVF
                     GVKNLVALEVASWPKWSQLLLLFVVRDFVQWNVHRFLHRVDFLWQFHKVHHS"
BASE COUNT          949 a          653 c          730 g         1007 t
ORIGIN      
        1 acatatcgag atccaggttc tggcagatac gcatggaaat gttgtttacc tttttgagcg
       61 cgaatgttca attcaacgtc gccatcaaaa attagtggag gaagctccct cagcaatcct
      121 cactcccgaa attcgttctg ccatgggtaa atgtgcagtt gatatttgtc ttgcatgcaa
      181 ttaccgtggt gccggtacag tggaatttct ggtggatgag caactgaatt tttatttcct
      241 cgaaatgaat acccgcctgc aggttgaaca cccggtaaca gaattgatta ccggaatcga
      301 tcttgttaag gaacaaatta aggttgcccg aggagaaaaa ctttcattct cacaaagcga
      361 tctgaagatt catggacatg cactcgaagt tcgggtttgt gcagaagatc cggataacaa
      421 cttcctcccg gaaatgggca aactgatcac ttaccgtccg cctgaaggtc ccggtgtgcg
      481 tgttgatgat ggttatgaag aaggaatgga gattcccatt tactacgacc cgatgatctc
      541 taaactgatc acctatggaa aggatcgcac cgaggctatc gaacgaatgg tccgcgctat
      601 ttccgaatat gaaatttcag gcgtcgaaac cactcttaaa ttttgtgcat tcgcaattca
      661 gcatgacgct ttcgttagtg ggcattttga taccaacttt gtaaagaatt atttcgttcc
      721 gggttccttg cataaggaaa tgacggatga aatggaggtt gcagcaatat ttgcatccgg
      781 acattcttct tctaaaccga aaattcaggc gaattccggg aattcagccg agagtttgtg
      841 gaaaaaaaga aaggaattga gataacctct ttgcgttttc tgcgttaaac aggggtaatc
      901 cgtattccca tgctgccaaa agggacgtat attattctga ttctccttct ttcattcctt
      961 cgggtaaatg gacaaaatcc cgattccctg agcaggtttc gtccgggtta tgtcaattcg
     1021 gtggtggtaa ataacgatac ccttcaccaa tattttcttt atgatgtaac cattactgca
     1081 tccgattctg tttacaaggc caaatacacc aggtttttgc gctatgtccg taaaacctat
     1141 ccttatgtga tgcttgcgaa acagcttgtt gcagagtacg aaaaggatct tgccgagaag
     1201 aactccaagc gtgccgaaaa aaaatacatc aatcaggaat ttgaagaatt aaaagaccgc
     1261 tttacggacg gtattgctgt tttgagtgtt aatgagggaa aagtactggt aaagcttatt
     1321 caccgcgaaa ccggaatgac agcctatgag attgcacaca aataccttgg aactgcacgt
     1381 gctttgattt ggcaggggat ttccagaacc ggaggcgcaa atcttaagct gactttcaac
     1441 ccggatgaag ttgatgacaa gattctcgaa ggaattgtga aaaaggtaga aaaaggggag
     1501 atctgggtgc caaaagaacc ttttgttcca gcaaatcgga aacaatacga aaaagaacgt
     1561 cgccaaaagt caagagaata caagcaggag cagaagaaaa aatcttccgc cgcatcaact
     1621 tcttcggtta aatagccgac ttgttcacaa tctgaatttc tagagcatat ttttttattt
     1681 ttactccatg tacaaagcag gtttacttct tgtatttatt tccctctttt ctgttagcgc
     1741 gactcaggca caaaaccaga aagtgtcctg cgattcagtt tatacctatg cagaaaatcc
     1801 gccacaatat cccggtggaa tgaaagcttt ttatgatttt ttgaaagggt ttcactatca
     1861 ggatgtaggt ccaaacgatc ctgtagttgg aaaattcaac atacatctta cactggatgc
     1921 agaaggaaaa cctatagatg ttcaggttac tccggaatcc gatcctggac ttgcacttaa
     1981 ggagtatctt atcaagatgc aaacatggac tcccgcttca acaaaggatg ggaaagtgtg
     2041 ttatttttta aaaatacccg cttacgtcca ttacaagtga tcaccactgc cgaaatcaaa
     2101 tctgtaaaat ccctgcacca gaagaaattc agggaggaga gcggtttgtt tttggttgaa
     2161 ggtgaaaaat tgcttcatga acttctgcat tccgaatggg agatcgaacg catctgtgcg
     2221 acttcttcgt ggctggataa aaacaagatc aatcagggaa ttataatcaa tgaagttaaa
     2281 tcatcagaat tggaacgcat tagcgcactc actcaaccca atgaagtact tgcagttgtt
     2341 cgtcaacgta agtttgattt taatctcaaa actgcacaaa acgggttgac aatattgctt
     2401 gatgatgttc gcgatccggg taaccttgga actattattc gtattgctga ttggtttggt
     2461 gtaaggcaaa ttgtatgttc acctcaatcc gttgaactct tcaatcccaa aacgattcaa
     2521 tctaccatgg gatctgcttt tcgaattccg gttgtttata cagacttact accactggtt
     2581 catgatctga aaaaggaggg tatcgcaatt tgtggggctg taatggaggg aaagaatgtg
     2641 ctttcatttg agtggaaaaa atcgacggct ctggtaatgg gtagcgagtc tcacggaata
     2701 aatgccagac tttctgcttt gcttgatgag cacgttacaa tcccgcgttt tggtgaagca
     2761 gaatcgctaa atgtttctgt tgcaacagga attttctgtg caatgtttaa tcggcagttt
     2821 tcatgatagg tgataaacat cactgtctac gtaatcttac tccggtaact ttgtatacat
     2881 gaaagagtac ggcgaattat ttttaaataa tttttttggg tatttcaatt accttaagga
     2941 tgagattctg tttaaaacgg agccttggta ccataattat ttttattggc taatcctgat
     3001 atctgttttc ttttggctgc tggaaatcat tgcaccatgg cgtcaaaggc aggcggtaat
     3061 tcgtaaggat ttctggctgg atggatttta catgttcttt aattttttcc ttttttcact
     3121 gatcggatac gctgctattt cttatgtttt tgtagagctg tttaatgatt ttctcggagt
     3181 gtttggcgta aaaaatctgg ttgctcttga agtagcttca tggccaaaat ggtcgcaact
     3241 gctattgctt ttcgtcgttc gcgattttgt tcagtggaat gtccaccgtt ttttgcaccg
     3301 tgtcgatttt ctctggcaat ttcataaggt tcaccattc
//
LOCUS       DAOMYG010000365         3337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10392_length_3337_cov_8.586837, whole genome shotgun sequence.
ACCESSION   DAOMYG010000365 DAOMYG010000000
VERSION     DAOMYG010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10392_length_3337_cov_8.586837"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2012
                     /locus_tag="PK610_11030"
     CDS             <1..2012
                     /locus_tag="PK610_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ36515.1"
                     /translation="GEPGVGKTAIAEGLAHRIVDGDVPENLKSKVIYSLDMGALIAGA
                     KYKGEFEERLKSVVKEVISAEGEIVLFIDEIHTLVGAGGGEGAMDAANILKPALARGE
                     LRAIGATTLNEYQKYFEKDKALERRFQKVLVDEPDSEDAISILRGIKEKYEAHHKVLI
                     KDEAIISAVELSQRYITDRFLPDKAIDLIDEAASKLRMEINSKPEELDELDRRIMQLE
                     IEREAIKRENDEKKLAALNQELANLNDQRDQFMAKWKAEKDVVDSIQQMKTGIEQLKL
                     EADQAEKNGDYGKVAEIRYGRVQESERKLEELKKQLLDLQANSKMIKEEVTSEEIAEV
                     VSKWTGIPVSKMIESEKEKLLRLEDELHKRVVGQEEAITAVADAVRRSRAGLQDQRRP
                     IGSFIFLGTTGVGKTELAKALSEFLFNDENAMTRIDMSEYQERHSVSRLVGAPPGYVG
                     YDEGGQLTEAVRRKPYSVVLLDEIEKAHPDVFNVLLQVLDDGRLTDNKGRMVNFKNTI
                     IIMTSNIGSHIIQENFDKMGKKDPEEVAEITKREVFELLKQSVRPEFLNRIDEVIMFK
                     PLDKSEIRNIVKLQLLQLGETLKKNDIRLHITEEAVKMISTLGFDPQFGARPVKRVIQ
                     KQVLNELSKSLLAGKVNREEVIVMDAIDNKIIFRRPIKIEEETTV"
     gene            2091..2885
                     /locus_tag="PK610_11035"
     CDS             2091..2885
                     /locus_tag="PK610_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HRJ36516.1"
                     /translation="MREKIYRIIFFTDTRAGRNFDLLLLYLILINFLVVVMESVPHLD
                     AQYGNEFYLIEWFFTGLFTLEYVIRILTSPQPFKYIFSFWGLIDLIAIIPGYLSVLVS
                     GYHYLQGIRIFRLIRVFRILRLTRFSAEARTLTVAIQNSAYKIVVFMMFMMVIVIILG
                     TIMYVIESGNPGFSSIPQAVYWAIVTVTTVGFGDVVPVTVLGKILASLTMLIGYVIIA
                     VPTGITAMEFHNATKTEEGKKECGHCSRTNRKDARYCYHCGNQFPE"
     gene            complement(2892..>3337)
                     /locus_tag="PK610_11040"
     CDS             complement(2892..>3337)
                     /locus_tag="PK610_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36517.1"
                     /translation="LLLHNDKEALNDLLVYSNACPDDLLTELKLSYVYLLLGDFPEGQ
                     FHLRRFEEKGKPDPNAKLIGQYILNFKRRMHLEIGVADIHLKSEYSEENLRHRAEIKE
                     KAFDLVGASEDYLMLYLLFEREEYQKRAEKIKLEILERHRRQNKM"
BASE COUNT         1069 a          611 c          747 g          910 t
ORIGIN      
        1 ttggagaacc aggtgttggt aaaacagcaa ttgccgaagg acttgctcac cggattgttg
       61 atggagatgt acctgaaaat ttaaaaagca aggtcattta ttcgctcgac atgggcgcat
      121 taattgctgg tgcgaagtac aaaggagaat ttgaagaacg attaaaatcg gtagtaaaag
      181 aagtgatcag tgcggaagga gaaattgtac ttttcattga tgagatacat actctagttg
      241 gcgcaggagg cggtgaaggc gcaatggatg ctgcaaatat tcttaaaccg gctttggcaa
      301 gaggtgaatt gagagcaatt ggtgcaacta cgctgaatga ataccagaaa tattttgaaa
      361 aagacaaggc gcttgaacgt cggtttcaga aagtacttgt tgacgagccc gattctgaag
      421 atgcgatttc catacttcgc ggaatcaagg aaaagtatga agcgcatcat aaagtgctga
      481 ttaaagatga agcaatcata tcagctgtgg agctttcaca acgatatatt acagatcgat
      541 tccttcccga caaagccatc gatttgattg atgaagcagc ttccaaactt cgaatggaaa
      601 tcaattccaa acccgaggaa ctggatgaac tggatcgcag aatcatgcaa ttggaaattg
      661 aacgggaagc cataaagcgt gaaaacgatg aaaagaaatt agcagcactt aaccaagagc
      721 ttgccaatct caatgatcaa cgcgatcagt ttatggccaa gtggaaagca gaaaaagatg
      781 tagttgattc tattcagcaa atgaaaaccg gaattgagca attgaaactg gaagcagatc
      841 aggctgaaaa aaacggtgat tacggtaaag ttgcggagat aagatacgga cgtgttcagg
      901 aatcggaacg caagttggaa gaattgaaaa agcaattgct ggatctgcag gcgaattcca
      961 aaatgataaa ggaagaagtt acctctgaag aaattgcaga agtggtttcc aaatggaccg
     1021 gaattcctgt ttcgaagatg atcgagagcg aaaaggaaaa gcttttacga ctcgaagatg
     1081 aacttcacaa acgtgtagtt ggtcaggaag aagcgatcac tgccgttgca gatgccgttc
     1141 gaagaagtcg cgcaggatta caagatcagc gtcgccccat cggatccttc atcttcctcg
     1201 gaacaaccgg tgttggtaaa acagaattgg cgaaagcgct cagtgaattt ttattcaacg
     1261 atgaaaatgc gatgactcgt atcgatatga gcgaatacca ggaacgtcat tctgtatcac
     1321 gacttgttgg agctcctccc ggatacgtgg gatacgatga aggtggtcaa ttaaccgaag
     1381 ctgttcgtcg caaaccatac agtgttgtat tgctcgatga aatagaaaaa gcacatcctg
     1441 atgtattcaa cgtattgctt caggttctgg atgatggaag gttaaccgac aataagggaa
     1501 gaatggtgaa ttttaaaaac acgatcatca tcatgacatc caacattggt tcgcatatca
     1561 tacaggagaa tttcgataaa atgggtaaga aagatccgga agaagtggct gaaatcacca
     1621 aacgtgaagt gtttgaatta ctgaaacaaa gtgtgcgtcc ggaatttctc aaccgtatcg
     1681 atgaagtgat catgtttaaa ccgctggaca aatcggaaat cagaaatatt gtaaaacttc
     1741 aattgctgca gcttggagaa accttaaaga aaaacgatat ccgtctgcac attacggaag
     1801 aagccgttaa aatgatcagc acattaggct ttgatcctca attcggagca agaccggtaa
     1861 aaagggtaat tcagaaacaa gttctcaacg aactttcgaa aagtctgctt gccggaaaag
     1921 tgaaccgcga agaagtcatt gtgatggatg ctatcgacaa caagattata ttcagaaggc
     1981 cgataaaaat agaggaagaa acaactgtgt aatcagctaa tgattcatcc ggattcataa
     2041 aaatttcgga tattgacagg cccggagcgt aacactaccg ggccttttat atgcgcgaga
     2101 aaatatacag aatcattttt tttaccgata cacgtgcagg tcggaatttc gatctccttt
     2161 tattatacct cattctgatc aactttttag ttgtggtgat ggaaagtgta cctcacctgg
     2221 atgcacaata cggaaatgaa ttttatctta tagaatggtt ctttactgga ttatttacac
     2281 ttgaatatgt aatcagaata cttacctctc ctcaaccgtt taaatatatt ttcagctttt
     2341 ggggacttat tgatttgatc gcaattattc ccggctatct gagtgttttg gtaagtggat
     2401 accattacct gcagggaatc cgaatattca gactcatcag ggttttccgt attttaaggt
     2461 taacacgttt ttctgcagaa gcaagaacac tcactgtggc tattcagaac agtgcttata
     2521 agattgtggt gtttatgatg tttatgatgg tgattgtaat tatcctcgga acaatcatgt
     2581 atgttattga atccggaaat cccggattca gcagtattcc acaggcagtg tattgggcta
     2641 ttgtaaccgt aacaacggtt ggatttggcg atgtggttcc ggttactgtt ttgggaaaaa
     2701 tacttgcatc gctaaccatg ttaatcggtt atgtgatcat tgctgttcca accggaatta
     2761 cggcgatgga atttcacaat gcgaccaaaa ctgaggaagg gaaaaaagaa tgtggacatt
     2821 gttcacgaac caacagaaag gatgccaggt attgttacca ttgcggaaat cagtttcccg
     2881 aataacatct ctcacatttt attctgtcgt cggtggcgct ctaaaatttc gagtttgatt
     2941 ttttctgcac gcttttggta ttcctcccgt tcgaacaaca aatacaacat caaataatct
     3001 tcggaggctc caacaagatc aaaggctttc tcctttattt cggcacgatg tcttaagttt
     3061 tcttctgaat attcgctttt caaatgaata tccgcaacac cgatttcaag gtgcattctg
     3121 cgtttaaaat ttagaatgta ttgtccaatg agctttgcat tcggatctgg ttttcccttt
     3181 tcttcgaaac gacgtaaatg aaattgcccc tccggaaaat cgcccaacaa aaggtaaacg
     3241 taacttaatt ttagctcggt taacaagtca tctggacaag cattactgta aaccagtaaa
     3301 tcatttaaag cttctttgtc gttgtgcaat aacaaat
//
LOCUS       DAOMYG010000366         3334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10407_length_3334_cov_6.827691, whole genome shotgun sequence.
ACCESSION   DAOMYG010000366 DAOMYG010000000
VERSION     DAOMYG010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10407_length_3334_cov_6.827691"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PK610_11045"
     CDS             <1..399
                     /locus_tag="PK610_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36518.1"
                     /translation="QEYDLMFRILKGGGKVGFTEELYTIRHNRPESISAMNIRRNWDS
                     YCNLRFEILKFISENFSKPLDHDYLQAYFEAIYMYSKVDFKASYSWFNKLVWVNFRLS
                     PSEIIPEMYCRLFNVFGFRIAQKILLVFGK"
     gene            389..1552
                     /locus_tag="PK610_11050"
     CDS             389..1552
                     /locus_tag="PK610_11050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ36519.1"
                     /translation="MENSARDILFLTDGIPPYVMGGMQRHSLEVCRQLLKHGFHVHLV
                     HCVQSGERLPDAGEVKGVIIQNESFTGSLTITGFHFPSPGFFPGHYLRNSLKYAKDIF
                     DSISNQNFDFIYAQGFTGWHFLRQKNRVTPVWVNFHGLNMFQRTIGLRSKLEAMMLRG
                     SVKENLKLADGVISLGGHLTEILNRKIGIDASKIKVHPNGISSQWIKSEGASIQDSNK
                     RRFLFVGRNDKIKQFDLIFGLANEFKDRAQFGFVGPFTPDVSQETGITFYGEIKKEEQ
                     LKAIYDQHDIIVCASISEGLPTTLLEGMARGLVVLTTPVGAASELINGNGIIVDGFDA
                     LAIKKGIQNILAMSETEFSNAKKRSLERVRCFTWGAIGEKVAGDIKQLSSKEN"
     gene            complement(1524..2462)
                     /locus_tag="PK610_11055"
     CDS             complement(1524..2462)
                     /locus_tag="PK610_11055"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRJ36520.1"
                     /translation="MSKKIFVTGGAGFIGSHLVQRLVNEGHKVVVADILLRGNKINPE
                     TFRKIQFHQADVRDSAAMLELSKGCDLIYHFAAVLGVDVVADNPVETMDTEIIGMRNI
                     CHAALTNGIKKIMYASTSGIYGHSAIDQTVTETINVDPRTSYAIAKRYNEIYLAAMYQ
                     EKGLESISVRFFNIYGPGQDERMVIPRFLEQARKNKPITVYGTGEQTRDFTYIDDTIE
                     ACMLLTEKIKGSEIYNIANEHEISIRDLAEKIVALSASKSPIQFLESPSKRYDYEVGR
                     RWGSSEKLFGITGFKPGIGLEEGLNYVLNSPLKRVV"
     gene            2548..2949
                     /locus_tag="PK610_11060"
     CDS             2548..2949
                     /locus_tag="PK610_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36521.1"
                     /translation="MSYLQSLFTDLLNGYALSDLPLFLVQLFTAALLGWGLSRFMNQE
                     SSESNPVLLAGVFAFLTAIVKFSFPLGLVLVALSVLAGFRWVNKAMNERSHLLFLLAA
                     AAGIGCGSGSVILTLIAYGVLVFPVLWFARK"
     gene            2949..>3334
                     /locus_tag="PK610_11065"
     CDS             2949..>3334
                     /locus_tag="PK610_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36522.1"
                     /translation="MKRKSNILLIYLLLGAGLAAAFLPSKSFVHFYHYTESASLPDAL
                     LTSDDITAPGNCMLLWSTDGSNWIKCKRISKSELKPSFGKTALSSIPTSFRWKAPMEG
                     LPVLPVLQYVVVREEDGAKLRSPVHY"
BASE COUNT          884 a          688 c          724 g         1038 t
ORIGIN      
        1 caagaatatg atttgatgtt tcggattttg aagggtgggg gtaaagttgg atttaccgag
       61 gaattatata caattcgcca taataggcct gagtctataa gtgctatgaa tattcgtagg
      121 aattgggact catattgtaa tttaagattc gaaattctta agtttatttc ggagaacttt
      181 tcaaagccac tagatcacga ttatcttcag gcatattttg aagcgattta catgtactct
      241 aaagtagact ttaaagcttc ctattcttgg tttaataagc ttgtttgggt taactttcgc
      301 ctttcgcctt ctgaaataat tcctgaaatg tattgcaggt tatttaatgt ttttggtttt
      361 agaatcgcac aaaaaatatt attagttttt ggaaaatagt gcaagagata ttctattttt
      421 aaccgacggt atcccaccct atgttatggg cggcatgcag cgtcattctc ttgaagtgtg
      481 caggcaattg ctgaaacatg ggtttcatgt gcacctggta cactgtgttc aatcaggtga
      541 acgtttaccg gatgccgggg aggtgaaggg tgtcataatt cagaatgaat ccttcaccgg
      601 ttcattaacc atcaccggat ttcattttcc ttcaccggga ttcttccccg ggcattacct
      661 gcgcaattca cttaaatacg cgaaggatat tttcgattcg atttcaaatc aaaactttga
      721 tttcatttat gcgcaaggct tcaccggatg gcattttcta cggcagaaaa atcgggtgac
      781 gcctgtatgg gtgaattttc acggattgaa tatgtttcag cgtacaattg gattgcgttc
      841 aaaactggag gcaatgatgt tgcgcggatc tgtgaaagaa aatctgaaat tggcggatgg
      901 ggtgatttcg cttggaggac acctcactga aattcttaat cggaaaatag gaattgatgc
      961 atcaaaaata aaagttcatc ccaacggaat ttcatcccaa tggatcaaat ccgagggggc
     1021 ttctatccag gatagcaaca aacgtcgttt tctattcgtt ggtcgcaatg ataaaatcaa
     1081 acaattcgat ttaatttttg gtttagccaa cgagtttaaa gatcgggctc agtttggttt
     1141 tgtaggtcct tttactccgg atgtttctca ggaaaccgga atcacgtttt atggggagat
     1201 aaaaaaagag gagcaattaa aagcgatcta tgatcaacac gatatcattg tctgcgccag
     1261 tatttctgaa ggactgccaa ctacattact tgagggtatg gcaaggggat tggtcgttct
     1321 caccactcct gtgggagcag cttctgaatt gataaacgga aatggaatta ttgtagacgg
     1381 gtttgatgct ttggcaatca aaaaaggaat tcagaacatc cttgcgatgt cggaaactga
     1441 attttcaaat gcaaaaaaaa ggtcacttga aagagttcga tgctttacct ggggtgcaat
     1501 cggtgaaaaa gttgcaggtg atatcaaaca actctcttca aaggagaatt aagcacatag
     1561 ttcagtcctt cttccaaacc gatacccggt ttaaatccgg taatgccaaa taatttctcc
     1621 gaacttcccc atctgcgtcc cacttcataa tcgtagcgtt tgcttggaga ttcaagaaat
     1681 tgaatgggtg acttggatgc cgataaagca acaatttttt ctgcaagatc acgaatgctg
     1741 atctcgtgtt cattcgcaat attatagatc tctgatccct tgatcttctc tgtaagcagc
     1801 atgcaggcct caatggtatc gtcgatatag gtgaaatcgc gggtttgctc tccggttccg
     1861 taaacggtga tgggtttgtt tttacgcgcc tgttccaaaa agcggggaat caccatgcgt
     1921 tcgtcctgac caggcccgta aatattgaaa aaacgtactg aaatggattc cagtcctttt
     1981 tcctggtaca tcgctgcaag gtagatttca ttatagcgtt tggcaatggc ataactcgtg
     2041 cgcggatcta cattgatggt ttccgtaacg gtttggtcga tcgccgaatg accgtaaatc
     2101 ccactggtag aggcatacat gatctttttg atgccgttgg taagggctgc atgacaaata
     2161 ttgcgcattc caatgatctc agtatccatg gtttccaccg gattgtcggc caccacatct
     2221 acacccaaaa cagctgcaaa atggtagata agatcacagc ctttggaaag ctcaagcatg
     2281 gctgctgaat cacgtacatc tgcctggtga aattgaattt ttcggaaggt ttccggattg
     2341 attttattcc ctcgtaataa aatgtcggct acaaccacct tgtgcccctc gtttaccagc
     2401 cgttgaacca aatgactacc tatgaacccg gccccgcctg ttacaaatat cttttttgac
     2461 atagcaataa ttagtgttgg tacaaaggta aatacactaa atcgtttaat ccccaccgaa
     2521 aaggttattt ttgtcaaaac ccctaatatg tcctacctac agtcgctttt tactgacctg
     2581 ctgaacggtt atgcgctttc tgatcttccg ctgtttttag ttcagctctt tactgccgca
     2641 ttgctgggat gggggctttc ccgttttatg aaccaggaat catcggaatc caatcctgtt
     2701 ttactggcag gggtatttgc ttttctcacg gctattgtaa aattttcttt tccgctcgga
     2761 ctcgttctgg tggctctctc cgttcttgca ggtttccgct gggtgaacaa ggccatgaat
     2821 gaacgttcgc atctcttgtt tctattggct gctgctgccg gtattggttg cggatcggga
     2881 tcggtgatac ttacactgat tgcatacgga gttcttgttt ttcctgtgct ctggttcgca
     2941 agaaaataat gaaaagaaaa tccaatatcc tgctcattta tcttctgctt ggtgcaggac
     3001 tggctgctgc atttttacca tcgaagtcat ttgttcattt ttaccactat accgaatccg
     3061 catctttacc cgatgcatta ctaacttctg atgacattac agcgcccgga aattgcatgc
     3121 tgctttggtc aacggatgga agcaattgga taaaatgcaa acggatttcc aaatctgagc
     3181 tcaaacctag tttcggtaag actgcactct cttcgattcc tacttctttt cggtggaaag
     3241 ctccaatgga aggtttacct gttctacctg ttcttcaata tgtggtagtc cgggaagaag
     3301 acggagccaa acttcgttct cctgtacact acca
//
LOCUS       DAOMYG010000367         3323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10462_length_3323_cov_9.131273, whole genome shotgun sequence.
ACCESSION   DAOMYG010000367 DAOMYG010000000
VERSION     DAOMYG010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10462_length_3323_cov_9.131273"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="PK610_11070"
     CDS             complement(<1..146)
                     /locus_tag="PK610_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004919419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRJ36523.1"
                     /translation="MKRERSEHLFEKAKNYFPGGVNSPVRAFRSVGGTPLFIQKGKGS
                     RIWDA"
     gene            270..1556
                     /gene="kynU"
                     /locus_tag="PK610_11075"
     CDS             270..1556
                     /gene="kynU"
                     /locus_tag="PK610_11075"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HRJ36524.1"
                     /translation="MEYRNTLEFAREADQQDPLRSYRQQFHFPQHQGTDVVYFTGNSL
                     GLQPKRTREHILQELDDWAKFGVEGHFYAKHPWFSYHENLTASNAAIVGALPSEVVCM
                     NQLTVNIHLLMVSFYRPTPLRYKILCEAKAFPSDQYALETQVQHHGFRAEDAILEIAP
                     REGEYTIRTEDILKVIEENKDSLALVMMGGVNYYTGQFFDIKTITAAAQKVGAYAGWD
                     LAHAAGNVELHLHDWNVDFAAWCSYKYLNAGPGSVSGAFVHEKHAHSKLPRFAGWWGH
                     DKSIRFKMEKGFQPMPGAEGWQLSNAPVLSMAAYKASLELFAEVGMPQLIKKSRLLSG
                     YLEFLIAEVNKAVSGKENTPLEIITPLEWNQRGAQISMVAHGQGKDLFNKLIAEGVIP
                     DWREPNVIRLAPVPMYNSFEDMFRFAEILTRILKSA"
     gene            1559..1900
                     /locus_tag="PK610_11080"
     CDS             1559..1900
                     /locus_tag="PK610_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36525.1"
                     /translation="MEPLLLFLFRHTSTAGGQGLEGELHLLQIAGITALVLGMISYQL
                     GKMKTNELLLHLGKALILGSVILNFSPLLQGESSQKTMTVALLFGLTGIILSSIIFFQ
                     IRPDSEEDEEH"
     gene            1937..3307
                     /locus_tag="PK610_11085"
     CDS             1937..3307
                     /locus_tag="PK610_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRJ36526.1"
                     /translation="MGKNVTIIGAGLVGSLLSIYLAKRGYKVTAYERRPDLRKNRISA
                     GRSINLALSDRGWRGLAGVGISEEIRKVAIPMKGRMMHPVSGELTYQPYGKDEQAIYS
                     VSRGGLNCVLMDLAEQHGVKIVYNEKCTDIDLDHAITHFENAETGKNTTVSSDHIFST
                     DGAFSAGRMQLQTRTDRFNYSQTYLDHGYKELTIPPTASGDFAMEPNALHIWPRGQFM
                     LIALPNMDKSFTCTLFFPFEGEKSFEALDTPEKMMSFFRETFPDAIPMMPTLKEDYFS
                     NPTSSLMIVKCFPWTYKDKLAMVGDSAHAIVPFYGQGMNCGFEDCVVLDELMSKHGLD
                     ASLENDSEVFKKVFSEYQSLRKPDGDAIADLAVMNFIEMRDKTADPKFLLQKKIEARF
                     STKYPEKWIPLYSMVTYSPHMRYSEALREGLRQENIMQNIMAMPGIEQKWDSEEVEKK
                     ILGMLS"
BASE COUNT          978 a          712 c          775 g          858 t
ORIGIN      
        1 gcatcccaga ttcgtgagcc ctttcccttt tggataaata gaggtgtgcc gcccacactg
       61 cgaaaggccc gcacaggaga gttcactcca ccgggaaagt aatttttagc tttttcaaac
      121 aggtgttcag agcgttcgcg tttcataatc ggaatacatt gggttgcaaa aataagcgtt
      181 gattggatag aacaattgaa aatctgatat ggatcagttc aacacgctgt tttcttcctt
      241 ttcattaagt ttgccgataa acaacaagca tggagtaccg taacaccctc gaatttgcgc
      301 gtgaagccga tcagcaagat cccttacgca gttaccgtca gcaattccat tttcctcaac
      361 accagggtac tgatgtggta tattttacgg ggaattcgct cggcttacaa cccaaacgca
      421 cgcgggagca cattttgcag gagctggatg actgggctaa gttcggggtg gaaggccact
      481 tttacgccaa acacccctgg ttttcgtacc atgagaacct tacagcaagc aatgcggcaa
      541 ttgtgggtgc attaccctca gaggtggtgt gcatgaacca gcttaccgtc aacattcatt
      601 tgctgatggt ttcgttttat cgacctacac cgctgcgcta caaaattctc tgcgaagcca
      661 aagcatttcc ttctgatcag tacgcactcg aaacacaggt gcagcaccac ggatttcgtg
      721 ctgaagatgc tatacttgaa attgcaccca gagaaggtga atacactatt cgtacagaag
      781 acatcctgaa agtaatagaa gaaaataaag attcgcttgc cttggtaatg atgggtggag
      841 tgaactacta caccggacaa ttcttcgata ttaaaaccat tactgcagcc gctcaaaaag
      901 tcggagcata tgccggttgg gatcttgctc atgctgcagg aaatgtggaa cttcatttgc
      961 acgactggaa tgttgatttt gccgcctggt gcagttacaa atacctcaac gccggacccg
     1021 gttctgtatc gggagcattt gtccacgaaa aacatgcgca tagcaaatta ccccgttttg
     1081 caggatggtg ggggcacgat aaatccatcc gcttcaaaat ggaaaaagga tttcaaccca
     1141 tgcccggagc agaaggatgg cagttgagta atgcacctgt tttatccatg gcagcttaca
     1201 aagcttcact cgaacttttt gctgaagtag gaatgccgca actcataaaa aaatcacgat
     1261 tgctttcggg ttatctcgaa ttcttaatcg ctgaagtgaa taaagccgtg agcggaaaag
     1321 aaaatacccc tctcgaaatt attacgcctc tcgaatggaa tcaacgcggt gcgcagattt
     1381 cgatggttgc acacggacaa gggaaagatc tcttcaacaa actgattgca gaaggagtaa
     1441 ttcccgattg gagagaaccc aatgtgattc gtcttgcacc tgtgcctatg tacaatagtt
     1501 ttgaggatat gttcaggttt gcagaaatac ttacaaggat tttgaaatcc gcataacaat
     1561 ggagccactg ctgctatttc tttttcgtca caccagcaca gccggcggtc agggtctgga
     1621 aggcgaactg catttgcttc agatagcagg aattactgca ttggttttag gaatgatcag
     1681 ctaccaattg ggtaaaatga aaacgaatga gcttcttctt catttgggaa aagcattgat
     1741 tttaggttcc gtgattctga attttagtcc gctgctgcaa ggagagtcgt cgcagaaaac
     1801 catgacagta gcattactgt tcgggctgac gggcatcata ctttcatcca ttattttctt
     1861 tcaaattcgt ccggacagtg aagaagatga agaacactga ttcgaaataa ctgttagaat
     1921 aaaaacacgc aaccccatgg gaaaaaacgt aacgataatt ggcgcaggac tggtaggctc
     1981 attgctttcc atttatttag cgaaaagagg atataaggta accgcttatg aacgtcgtcc
     2041 tgaccttcgc aaaaacagaa tttctgcagg aagatcgatc aatcttgcgc tcagcgacag
     2101 aggttggaga ggcttggccg gtgtaggcat ttcggaagag atccgcaaag tggcgattcc
     2161 catgaaagga agaatgatgc atccggtgag tggagaactc acctatcagc cttatggaaa
     2221 agacgagcaa gccatctatt ccgtttcgcg cggaggactg aattgtgtac tgatggatct
     2281 ggcagagcaa catggagtaa agatcgtata caatgaaaag tgtacggata tagatcttga
     2341 tcatgccatt actcattttg aaaatgcaga aacaggaaaa aacacaacag taagcagcga
     2401 tcacattttt tccactgatg gagccttttc agcaggaagg atgcaattgc agacacgtac
     2461 cgatcgattt aattattccc aaacctatct cgatcacggt tacaaagaac ttaccattcc
     2521 cccaactgcg agcggagatt ttgccatgga acccaacgcg cttcacatct ggccacgcgg
     2581 tcagtttatg ctgattgcgc ttccgaatat ggataagagt tttacgtgta cattgttttt
     2641 cccttttgaa ggagagaaat cgtttgaagc attggatacg cccgaaaaga tgatgagctt
     2701 cttcagggaa acattcccgg atgccattcc gatgatgccc accctgaaag aagattattt
     2761 cagcaatccc acttcatcgc tgatgattgt taaatgtttt ccatggacct ataaagacaa
     2821 actcgccatg gttggagatt ctgcacacgc cattgtaccg ttttacggac aaggaatgaa
     2881 ttgtggattt gaagactgcg tggtactgga tgaattgatg agtaaacatg gattggacgc
     2941 gtcactggag aatgattccg aagtattcaa gaaagtgttt tcagaatacc agagcctccg
     3001 caaaccagat ggtgatgcca ttgctgattt ggccgtgatg aactttattg agatgcgcga
     3061 caaaactgct gatccaaaat tcctcttgca gaaaaagatc gaagcacgat tcagcacaaa
     3121 atacccggag aaatggattc cactttattc gatggtaacc tattcgccac acatgcgcta
     3181 ttctgaggca ttgcgcgaag gattacgtca ggaaaacatc atgcaaaaca ttatggcaat
     3241 gcccggcatt gaacaaaaat gggatagcga agaagtggaa aagaaaattt tagggatgtt
     3301 gagctgagga atttctttct gag
//
LOCUS       DAOMYG010000368         3322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10467_length_3322_cov_9.031833, whole genome shotgun sequence.
ACCESSION   DAOMYG010000368 DAOMYG010000000
VERSION     DAOMYG010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10467_length_3322_cov_9.031833"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PK610_11090"
     CDS             complement(<1..706)
                     /locus_tag="PK610_11090"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRJ36527.1"
                     /translation="MRILEVEGSTNIAVEGITLDSRKVRPNWMFVAIKGLQSDGHDFI
                     NKAIELGADCIICEQIPSERHPHVTYVRVNDAAASLGVMAAEFYDHPSEKLKLIGVTG
                     TNGKTTTVTLLYSLFKRMGYKTGLLSTVVNRIHNETVTATHTTPNAIELNALLREMVD
                     AGCMYCFMEVSSHAVHQKRIAGVRFAGAVFTNITHDHLDYHGTFDEYLKAKKGFFDML
                     SADAFALVNKDDYHGEE"
     gene            complement(736..2859)
                     /locus_tag="PK610_11095"
     CDS             complement(736..2859)
                     /locus_tag="PK610_11095"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HRJ36528.1"
                     /translation="MKEKNDDRKDMMRRMYLVYLVLCVFALVVVVKVFRIQMDTVSVI
                     GQPEGLLTDLRTIEAVRGNIYSDNGSLLATSIPVYEVRMDMGADGLTDEIFFDNVDSL
                     AYKLSRLFPDKPKARFYQDLIEARKNGNRYYLVRAKVKHPELQAMKKFPIFKLGKNKG
                     GLIVVKENKRSRPYGLLAARLIGYERPDPDNPQFISRIGLEGAFSKELSGTDGKQLMK
                     KVGLQWRPVSDNYEVNPVDGADVFTSIDINIQDVAENALLSQLQKYEAKSGCVVLMEV
                     ETGMVKAMANLSIGEDGQYYEHFNHAIGTAIEPGSTFKTASLMVGFEDGMFKPSDSVK
                     TGKGEYSYYNIKMKDTKPHGTITIKHALEVSSNIGCSKPVVEAYSRDPKRFIEGLKKM
                     GIGELLGLDIAGEAKPYLKDPSNRNEWSGVTLPMMAIGYESKLAPIQILTFYNAIANN
                     GMMVKPQFVQDIRRNGKIVKKYKPIVMKEKICSEKTLNMIRPMLEGVVDTGTAMNLKA
                     ANFSIAGKTGTAQIHSKGAYNKEKYLASFVGYFPADAPRYSCIVWVMEPNVLTGYYGN
                     AVAGNVFKEIADKMYARSLEVSERERESLMASNELPVSKSAHQKDLTEIFKTLKIASS
                     TGDEGAEWVRPIADGEKRIVLEKKAIAENKVPTVIGMGLKDALFLLENRGLVVKVKGS
                     GKVKRQSLPEGKAIQQGETIEIELM"
     gene            complement(2864..>3322)
                     /locus_tag="PK610_11100"
     CDS             complement(2864..>3322)
                     /locus_tag="PK610_11100"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HRJ36529.1"
                     /translation="RARRESKIKKRKTPHSGKERNMNKYKVAKKESTKKARKEPRVVR
                     SFRDVLNGNVLTRDYVVDNLPYFFFLTVLMLVYIAMGYTTDKNARRIERIESELVELN
                     SEYISVKTELNLISRQSQIADSTASIGLFEMKDESPRVIRVSENQLKTIY"
BASE COUNT          874 a          775 c          613 g         1060 t
ORIGIN      
        1 tttcttcgcc gtggtaatca tctttattta ccaaagcaaa agcatcagcg ctcagcatat
       61 caaaaaagcc ctttttcgct ttcaggtatt catcaaaggt tccatgataa tcgagatgat
      121 catgagttat attggtaaat acagcaccgg caaaacgaac gccggcaatt ctcttctgat
      181 gaacggcgtg tgagctcact tccataaagc aatacataca acctgcatcc accatttcac
      241 gcaacaaagc atttaactca attgcattgg gtgtggtatg ggttgctgta accgtttcgt
      301 tatggatgcg atttactacc gttgaaagta aacccgtttt ataacccatt cgcttaaaca
      361 aactgtacaa aagcgtaaca gttgtcgttt ttccgttggt accggtaaca ccaatcagtt
      421 ttagtttttc agaaggatgg tcgtagaatt ccgcagccat tactccaaga gaagcagcag
      481 cgtcattcac gcgaacataa gttacatgcg ggtgacgctc agacggaatc tgttcgcaga
      541 taatgcaatc tgctcccagt tcaattgcct tattaatgaa atcatgccca tccgattgca
      601 aacctttaat ggcaacaaac atccaattgg gacgaacctt tcttgagtcg agcgttattc
      661 cctctactgc aatattggtt gaaccctcta cctcgaggat gcgcacactg tataatatgt
      721 cgcgtaataa cttcattaca tcaactcaat ctcaattgtt tcaccttgtt gaatggcctt
      781 tccttcagga agtgattggc gttttacttt tccactacct tttactttta ccaccagacc
      841 tctgttctcc aataggaaaa gcgcatcttt taatcccatt ccaattacag ttggaacttt
      901 attctccgca attgcttttt tctccaatac aatccgcttt tcaccatcag caatgggacg
      961 cacccattcc gcaccttcat cgccggttga agaagcaatt ttcagtgtct tgaatatttc
     1021 ggtcaggtct ttttgatggg cagacttact aacaggtaat tcgttcgatg ccataagaga
     1081 ttcacgctcc ctttcgctta cttcgagtga acgcgcatac attttatctg caatttcctt
     1141 aaacacattt cctgctactg cattaccgta atagcctgta agcacatttg gttccattac
     1201 ccaaacaata caactatacc gtggtgcatc agcaggaaaa tacccaacaa atgatgcgag
     1261 gtatttttct ttgttgtatg ctcccttact atgtatctga gcagttcctg tttttcctgc
     1321 aatagaaaag tttgctgctt ttaaattcat tgctgtaccg gtatcaacca caccttccag
     1381 cattggacga atcatattca gcgttttctc actgcagatt ttttccttca ttacgatagg
     1441 cttgtacttc ttcacaattt ttccgttgcg acggatgtcc tgtacaaact gaggcttaac
     1501 catcattccg ttattggcaa tggcattata aaaagtgaga atttgaatgg gagcaagttt
     1561 agattcatat cctatagcca tcataggcaa ggttacaccc gaccattcat ttctattgga
     1621 aggatctttt aaataaggct ttgcttctcc tgctatatca agaccaagta attcacctat
     1681 tcccatcttc ttcagtcctt cgataaaccg tttcggatcg cgactgtatg cttccacaac
     1741 aggtttagaa caaccgatat tggaagagac ctcaagcgca tgtttaatgg taattgttcc
     1801 atgcggtttg gtgtccttca tttttatatt gtaatagctg tattctccct tgcctgtctt
     1861 tacggaatca gaaggcttga acattccgtc ttcgaaaccg accatgagcg atgcggtttt
     1921 gaatgttgaa ccgggctcaa ttgctgttcc gatggcgtga ttgaaatgtt cgtaatactg
     1981 gccgtcttca ccaatggaaa gatttgccat tgctttaacc attccggttt ctacttccat
     2041 caatacaaca caaccacttt tggcttcgta tttctggagc tgacttaata gcgcattttc
     2101 ggcaacgtcc tgaatattga tatctatact ggtaaaaaca tcagcaccgt cgaccggatt
     2161 tacttcgtaa ttatcagaaa ccggacgcca ttgcaaccct acttttttca tgagttgttt
     2221 tccgtctgtt ccgcttaatt ctttcgaaaa cgctccttcc aatccgatac gggaaatgaa
     2281 ttgcggatta tcgggatcgg gacgctcgta accaatgagt cgtgcggcga gtaaaccgta
     2341 gggacgactt cttttgtttt cttttactac aattaatcca cctttgtttt ttcccaattt
     2401 gaaaatggga aacttcttca tcgcctgcag ttcaggatgc ttaacttttg ctcttaccag
     2461 gtaataacgg tttccgtttt ttcttgcttc gataagatcc tggtaaaaac gcgcttttgg
     2521 tttatccgga aacaaacggg aaagtttata agccagcgaa tccacattat cgaagaagat
     2581 ctcatctgtt aagccatcag ctcccatatc catgcgcact tcataaacag gaattgaagt
     2641 agcaagcaga cttccgttgt cggaataaat atttccgcgc actgcttcaa tggttcgcaa
     2701 atcagtaagc aatccctccg gctgaccaat tacagaaacg gtatccattt gaatgcggaa
     2761 aacctttacc acaacaacca gcgcaaaaac gcaaagcaca agatatacca ggtacatcct
     2821 ccgcatcatg tcttttctat cgtcgttttt ttctttcact cgttcagtaa attgttttca
     2881 attgattttc ggaaacacgt attactcttg gcgattcatc cttcatttca aatagtccga
     2941 ttgaagcagt tgaatcagcg atctgtgatt ggcggctgat aagattgagt tccgttttaa
     3001 cactgatgta ttcagaattc aactccacga gttcgctttc aattctttca atgcggcggg
     3061 catttttatc ggtagtgtag cccattgcga tgtacaccag cattaacaca gtgagaaaaa
     3121 agaagtatgg aagattgtca accacataat cgcgggtgag aacatttccg ttgagtacgt
     3181 cgcgaaatga acgcacaaca cgaggctctt tacgggcctt tttggtgctt tctttttttg
     3241 cgactttgta cttgttcata tttctttctt ttccgctatg cggagttttg cgcttcttga
     3301 tcttggattc tcttcttgct ct
//
LOCUS       DAOMYG010000369         3319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10476_length_3319_cov_12.138174, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000369 DAOMYG010000000
VERSION     DAOMYG010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10476_length_3319_cov_12.138174"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PK610_11105"
     CDS             <1..873
                     /locus_tag="PK610_11105"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HRJ36530.1"
                     /translation="VNSDCITRLAAPRKSMKDYFYKNADADEMIFIHKGKGKLRTFMG
                     NIDFEYGDYLIIPRGMIYQMEFEDDNNRLLFLESYHPIYTPKRYKNSHGQLLEHSPFC
                     ERDFKLPSEIETHDEKGDFIIKIKKEGMIHEVVYATHPFDVIGWDGYNFPWGFSIHNF
                     EPITGRVHQPPPVHQTFETSAFVVCSFCPRLYDYHPAAIPAPYNHSNIDSDEVLYYVD
                     GDFMSRNNIEQGHITLHPKGIPHGPAPGAMERSIGQKETQELAVMIDTFRPLMVTEEA
                     LRIDDGQYYKSWTE"
     gene            936..2096
                     /gene="hppD"
                     /locus_tag="PK610_11110"
     CDS             936..2096
                     /gene="hppD"
                     /locus_tag="PK610_11110"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HRJ36531.1"
                     /translation="MAKEVKNVEYGLEKIFEGAQDFLPLLGTDYVEFYVGNAKQAAHY
                     YKTAFGFQSHAYSGLETGNRERVSYVLKQDKIRIVLTTALNSNSPIGEHVKKHGDGVK
                     VVALWVEDARKAFEETTKRGAKPFMEPVVEKDEHGEVVRSGIYTYGETVHMFVERKNY
                     KGAFLPGFVEWKSDYNPSSVGLKFIDHMVGNVGWNQMNTWVKWYEDVMGFVNFLSFDD
                     KQIHTEYSALMSKVMSNGNGRIKFPINEPAEGKKKSQIEEYLDFYEDSGVQHIAVATD
                     DIIKTVTALRERGVEFLPPPPQAYYDEIPARLGVHKEMMKEDIKELQKLSILVDADEE
                     GYLLQIFTKPVEDRPTLFFEIIQRMGAKGFGAGNFKALFESIEREQALRGTL"
     gene            2412..2840
                     /locus_tag="PK610_11115"
     CDS             2412..2840
                     /locus_tag="PK610_11115"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36532.1"
                     /translation="MKTIFVFLTSTFLLISCSGEKSGKETSPADSVGHTIEVKPFNPA
                     ENMKEGTNEAFHANGKLKMKGDVVDGKRHGVWTTWYENGVKWSENNYSYGILEGKTVS
                     YFPNGQVQYIGYYSNDIKTGIWMFYNEQGELVNEDNYTKK"
     gene            2837..>3319
                     /locus_tag="PK610_11120"
     CDS             2837..>3319
                     /locus_tag="PK610_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HRJ36533.1"
                     /translation="MIPRDTVELITETARIEDVVGDFVALKKRGVNMIGLCPFHNEKT
                     PSFNVSPTRGIFKCFGCGKGGNSVNFIMEHEHVSYPDALRYLARKYNITIEEEQRSDE
                     EMQQDTERESLYVVSTFAQKHFTHNLQETEEGRSIGLSYFRERGFSLPTIEKFQLGYA
                     M"
BASE COUNT         1046 a          685 c          757 g          831 t
ORIGIN      
        1 gtaaacagcg attgtattac cagactggca gctccccgca aatcaatgaa ggactatttt
       61 tacaagaacg cggatgctga tgaaatgatc ttcattcaca agggaaaagg caaactgcgc
      121 acctttatgg ggaatatcga cttcgaatac ggcgattatc tgattattcc gcgtggtatg
      181 atttaccaga tggagtttga agatgataac aaccgtttgc tgttcctgga aagctatcat
      241 cccatttata ctccgaaacg ctataaaaat tcgcacggac aattactgga gcattcaccg
      301 ttttgcgaac gcgattttaa gttgccttcc gaaatagaaa cgcatgatga aaaaggcgac
      361 ttcatcatca agattaaaaa agaaggaatg atacacgaag tggtgtacgc cactcatccc
      421 tttgatgtga ttggttggga cggatacaat ttcccctggg gattttccat ccacaacttc
      481 gagcccatta ccggacgcgt acaccagcca ccaccggtac atcaaacatt tgaaaccagc
      541 gcatttgttg tttgttcgtt ttgtccgcgt ctatacgatt atcaccccgc tgcaattcct
      601 gcgccctaca atcacagcaa tatcgacagc gatgaggttt tgtattatgt agatggtgat
      661 tttatgagcc ggaataatat tgagcaagga cacatcacgc tgcatccgaa aggaattccg
      721 cacggacctg cacccggagc aatggagcgt tccataggac aaaaagaaac acaagaactc
      781 gctgttatga tcgatacgtt tcgtccgctg atggtaaccg aagaagcctt acgcattgat
      841 gatggtcagt attacaagag ctggacagaa taacattcag caaaaaagaa attcacttat
      901 caccaaactt ccgcaaggaa aaaacaaaat aagccatggc taaagaagta aaaaacgttg
      961 aatacggtct tgaaaagatt tttgaaggcg cgcaggattt tctgcctttg ttgggaactg
     1021 attatgtaga gttttatgta ggcaatgcca aacaggcagc acactattac aaaactgcat
     1081 tcggatttca gtcacacgcc tactccggtc ttgaaaccgg caatcgcgaa agagtttctt
     1141 atgttttaaa acaagacaaa atacgcattg tgcttaccac cgcactcaac agcaattcgc
     1201 cgatcggaga gcatgtaaaa aaacacggcg acggagttaa agtagttgca ttgtgggttg
     1261 aagatgcacg aaaagcattt gaagaaacca ccaagcgtgg cgcaaaacca tttatggaac
     1321 cggttgtaga aaaagacgaa cacggagagg ttgtgcgttc aggaatttac acttacggag
     1381 agacggtgca tatgtttgtt gagcgaaaaa attacaaggg cgcatttcta cccggatttg
     1441 tagaatggaa aagcgattac aatccttcat ccgttggctt gaaattcatc gaccacatgg
     1501 tgggcaatgt aggatggaac cagatgaata cctgggtaaa atggtacgag gatgtgatgg
     1561 gttttgtcaa tttcctctcc ttcgacgaca agcagatcca tacggaatac tctgcgctga
     1621 tgagtaaggt aatgagcaat ggaaacggtc gaatcaaatt cccgatcaac gagccggcag
     1681 aaggtaaaaa gaaatcgcag atcgaagagt atctggattt ttacgaagat tccggagtgc
     1741 agcatattgc cgttgctacc gacgacatca tcaaaactgt aaccgcactc agagagcgtg
     1801 gagtagaatt cctaccaccg ccaccgcaag cgtattacga tgaaattccc gcacgtttgg
     1861 gtgtgcataa agaaatgatg aaagaagaca tcaaggaatt acaaaaatta tccattcttg
     1921 tggatgcaga tgaagaagga tatctcctgc agatctttac caagccggtg gaagatcgtc
     1981 caaccctttt cttcgagatc attcaacgca tgggcgcaaa aggatttgga gcaggtaatt
     2041 ttaaagcact cttcgaatcc atcgaacgcg agcaggcgct gagaggaaca ctgtaaacta
     2101 aatcgattac acgaaagccc cgaccaatca tcggggcttt tgcattcgtt aagaattgat
     2161 caaaatagaa ttcaacattt caccgctctg gctgtttttc tgctaacttg ccttatgttt
     2221 tcattgctaa gatcactgct attgctcctt ttgcccttaa ctgcattagc acaggatagt
     2281 acgggtattg tgaatcagaa agaaaacgag ccgggtgtgc tcaggtattt tcttttagct
     2341 gtgcttgttg ttggcttgat cgtaatgtat tttgaacgca gacaaaaaca gaaattaaat
     2401 aacaatcaag aatgaaaacc attttcgttt ttctcacatc cacttttctt ctgatctctt
     2461 gttcaggcga aaaatccggt aaagaaacaa gtcctgcaga ttccgttggt catactatcg
     2521 aggtgaagcc tttcaatccc gcagaaaaca tgaaagaagg aaccaatgaa gccttccatg
     2581 cgaacggtaa gctgaaaatg aagggtgatg tagtcgacgg aaaacgtcac ggtgtgtgga
     2641 caacctggta tgagaacggg gtgaaatgga gtgagaataa ttattcctat ggaatattgg
     2701 aagggaaaac tgtaagttat tttccgaacg ggcaggttca gtacatagga tattacagca
     2761 atgatatcaa aacaggaatc tggatgtttt acaatgaaca gggcgagctt gtaaacgaag
     2821 acaactacac gaaaaaatga ttccacgcga caccgttgaa ctaatcaccg aaaccgcacg
     2881 cattgaggat gtggtgggtg attttgttgc gttgaaaaaa cgtggtgtca atatgatcgg
     2941 attatgtcca tttcacaatg aaaaaacccc ttcatttaac gtttcaccca ccaggggaat
     3001 ctttaagtgt tttggttgcg gaaaaggagg caattcggta aacttcatta tggagcacga
     3061 acacgtttcg tatccggatg ccctgcgtta tctcgcacgc aagtacaaca tcaccattga
     3121 agaagagcag cgcagtgatg aagaaatgca gcaggacaca gagcgggaat cgctttatgt
     3181 ggtaagcacg tttgcacaaa aacatttcac ccacaacctg caggaaaccg aagaaggaag
     3241 aagcatcgga ctctcttatt tcagggaacg cggattttct cttccaacca ttgagaagtt
     3301 tcagttaggt tatgcaatg
//
LOCUS       DAOMYG010000370         3311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10509_length_3311_cov_11.188882, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000370 DAOMYG010000000
VERSION     DAOMYG010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10509_length_3311_cov_11.188882"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3311
                     /locus_tag="PK610_11125"
     CDS             <1..>3311
                     /locus_tag="PK610_11125"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRJ36534.1"
                     /translation="VMGLTLNGQSYFRENLKLVSNLSGGTVRDILGDPEKSILAYAKA
                     YELLMQQYAGDFPSTGNLFNSKLHEAVLFQLSEIPDSGAVNAYARDAQVYQIFRFLNE
                     KSEAKKYGFIPRNIDLQEHYGENFRILSAQKIIFSEEGITNADGAVYRPSGSQLRSPD
                     YGPALWNPAPSCNYSSRNGVAVSAITIHTVQGSYAGAISWAQNCNSNVSYHYVIRSSD
                     GQITQMVLEANKGWHVGSENPYTIGYEHEGYVNNPSWYTTAMYNASAALSRDICNSGY
                     GIPPLRTYYGAASSGTNVLGGCTKIKGHQHYPNQTHTDPGINWNWELYYRLINNNPTT
                     NTLTAGSGTIYDSGGAGGNYANDERYITTIAPAGATSVTITFSSFNTEANWDYLFVYD
                     GNSINAPRIGTYTGTNSPGTITGTTGTLTLEFRSDCSTTASGWVANWTSNSTPPPPGD
                     NTPPSTSVATPSTWVTQNFTATFADADNSGGSGIQKAYYQVIDYDGLDWRANASNGFF
                     SDNFDLPVLHADWYTVSGTWNVVNGELVQSDASLNNTNAYAFIDQSLSNRYLYHWAGK
                     MAGAGTNRRAGLHYFCDDPTLENRGNSYFVWFRLDNDKVQLYEVINDVFVMLDEVDFN
                     FNVSQWYDFKVIYDRITGLHQVYIDNSLVQSFTDNTPIATGDYISFRSGNAEYTVNNL
                     KVYRSRFPNVQVSIGPSGDIRYQNANPTTPSGRVKSIVQDNAGNLSSTAFQDVNVDWT
                     APDAPSQVNDGPAADISSTTNANELEANWTSAFDQHSGIARYWYSIGTSPGLSDVKPW
                     TDNFWYDTVRVTGLNLNYGTIYYFNVCAENGAGLLSDTISSNGQLVQSPSLPPTANFN
                     IQNTFICAGESISFQNTSQNASTYTWNFTGGNPSNSTNQHPVVTYTTSGTYQVQLTAS
                     GPGGTDIITQNISINVSQPNSSSFAVTDTLVYLPNAFVGFTNLSSNANGYVWNFGDGG
                     NSNGVNPWHIYSAAGIYEAVLIAVNDACPADTSTKTIYVQGSNSIESNTAPQMNVHLN
                     SDLLIVRLRNMKSGIYQYAVYDTGGKRIISGNWTIGAVVSQNEIPFSKQAGGLYFIEV
                     KNETEQIRSGFVNPV"
BASE COUNT         1014 a          766 c          681 g          850 t
ORIGIN      
        1 cgtaatggga ttaacgttaa atggacaatc atatttcaga gagaatttaa aacttgtttc
       61 aaacttatcc ggaggtactg ttcgcgacat tttgggagat cctgaaaaaa gtatacttgc
      121 ctatgcaaaa gcctatgaat tactgatgca gcaatatgcc ggtgattttc cgtccaccgg
      181 aaatttattt aactccaaac tccatgaagc tgttttattt cagctttcgg aaatccccga
      241 ttcgggagca gtaaatgcat acgcaagaga tgcgcaggtt tatcagatct tccgttttct
      301 caatgaaaaa agcgaggcga aaaaatatgg attcattccg cgtaatatcg atcttcaaga
      361 gcattacgga gaaaacttcc ggattctgag tgcgcaaaaa ataatttttt cggaagaggg
      421 aattacaaat gcagacggag ccgtatatcg tccaagcggc agccaattgc gttcacccga
      481 ttatggtcct gccttgtgga atccagctcc ttcctgtaat tacagttcca gaaatggtgt
      541 cgctgtttcg gccattacca tccataccgt acagggatcc tatgcaggag caatatcatg
      601 ggctcaaaac tgcaattcga atgtctccta tcattacgta attcgttcga gcgacggaca
      661 aataacacaa atggtactcg aagcaaacaa aggttggcat gttggctctg aaaatccata
      721 taccatcgga tacgaacacg aaggatacgt gaataatccc agttggtaca ccacagcaat
      781 gtataatgca tccgccgcat taagtcgcga catctgcaat tcgggatacg gaattccacc
      841 cttgcgcacc tattacggag cagcaagttc aggaacaaat gtattgggag gatgtacaaa
      901 aattaaaggt caccaacact accccaacca aacacacacc gatcccggta taaattggaa
      961 ttgggaattg tattaccgat taatcaataa caacccaacc accaacacat taacagcagg
     1021 cagtggaacg atttacgatt ccggtggcgc aggaggaaat tacgcgaacg acgaacgcta
     1081 cattaccacc attgcacctg caggagctac ttctgttacc attacatttt catcattcaa
     1141 cactgaggcc aattgggatt acttatttgt ttacgacggc aactccatca atgcaccacg
     1201 catcggcacc tatacaggta ctaattcgcc gggaacaatt acaggaacaa ccggaacgct
     1261 tacacttgaa ttcagaagcg attgctctac caccgcttca ggatgggtcg ccaattggac
     1321 cagcaattcc acaccacctc ctcccggcga taatacacct cctagcacaa gcgtagcaac
     1381 tccctcaaca tgggtaacgc aaaattttac agcaacattt gccgatgctg acaacagtgg
     1441 tggatcagga atacaaaaag catattatca ggtaatcgat tacgatggat tggattggag
     1501 agcaaatgca tccaatggat ttttcagcga taattttgat ctacctgttt tgcatgccga
     1561 ctggtacacg gtttcgggaa catggaatgt tgtaaacgga gaacttgtac aatccgatgc
     1621 aagcctgaac aacaccaatg cttacgcatt cattgaccaa agtctttcga atcgctacct
     1681 ctaccactgg gcaggaaaaa tggcaggagc aggaacaaac cgacgtgcag gtttgcatta
     1741 tttctgcgac gatcctacct tggaaaacag aggcaattcc tatttcgttt ggttcagatt
     1801 ggataacgac aaagtgcaat tgtacgaagt catcaatgat gtttttgtaa tgctcgatga
     1861 agttgacttc aatttcaatg tgagccaatg gtacgatttc aaagttattt atgatcgcat
     1921 tacaggtctg caccaggttt acatcgataa tagtcttgtc caatctttta cagataacac
     1981 accgattgcc acaggagatt atatttcatt tcgctccgga aatgcagagt acacagtaaa
     2041 caatttaaaa gtgtaccgtt cacgttttcc gaacgtacag gttagcatag gaccatcagg
     2101 cgacattcgt taccagaatg caaatccgac tacaccatcc ggaagagtga aatcaatagt
     2161 gcaggacaat gcaggcaact tgtcttccac cgcttttcag gatgtgaatg ttgactggac
     2221 cgcgccggat gcgccatcac aggtaaatga tggtccggct gctgatatca gctcaacaac
     2281 caatgccaat gaactcgaag caaactggac atccgccttc gatcagcatt cgggaattgc
     2341 gcgttattgg tattccatcg gaacaagtcc gggattaagt gatgtaaaac catggaccga
     2401 caatttctgg tacgacaccg ttcgcgtaac cggattgaat ctgaactacg gtacgatcta
     2461 ttatttcaat gtatgcgcag aaaatggagc aggattactt tccgacacta tttcatccaa
     2521 cggacaactt gttcaatcgc ccagtttacc tcctacagca aacttcaaca ttcagaacac
     2581 atttatttgt gcgggtgaaa gcatcagttt tcaaaacaca tcgcaaaatg cctccaccta
     2641 tacctggaat tttaccggag gaaatccttc caattcaacc aatcagcatc ctgtagttac
     2701 ctacaccact tccggaactt atcaggttca acttacagca agtggtcccg gaggaacgga
     2761 catcatcaca caaaacattt ccatcaatgt aagtcaaccc aattcctcat cctttgctgt
     2821 aacagacacg cttgtttatc ttcccaatgc atttgttggt ttcaccaatt tgagcagcaa
     2881 tgcaaacgga tacgtctgga atttcggtga tggaggaaac agtaatggcg taaatccctg
     2941 gcatatttat tctgcagcag gaatttatga agcagtttta attgccgtta atgatgcttg
     3001 tccggccgac accagcacca aaaccattta cgtgcaaggc agcaattcca ttgagagcaa
     3061 cactgcaccg caaatgaacg tgcacctgaa ttcggatcta ttaattgttc gtttgaggaa
     3121 tatgaaatcc ggaatttatc agtacgctgt ttatgacaca ggtggcaaac gaatcatcag
     3181 tggaaactgg accatcggcg cagttgtttc gcaaaatgaa attccatttt ctaagcaggc
     3241 cggcggattg tattttattg aagtaaaaaa tgaaacggaa caaatcagat ccggattcgt
     3301 gaatcctgta c
//
LOCUS       DAOMYG010000371         3305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10543_length_3305_cov_8.402769, whole genome shotgun sequence.
ACCESSION   DAOMYG010000371 DAOMYG010000000
VERSION     DAOMYG010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10543_length_3305_cov_8.402769"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PK610_11130"
     CDS             complement(<1..483)
                     /locus_tag="PK610_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36535.1"
                     /translation="MEEIKASSGSDSVKVYALAELAVKAAKKSMDDGLSVITEMEMIS
                     KESPSLSAFTKVNKGEVYLKNSSPEKALENFQVAFRYYDKSNNQKMIVGLLVRQAKAY
                     QLLNNHDKVIEKYSEAYRLAEQKAGVEVFIEVVNDYYGYLGKRGDRAAQFKLLSDQLT
                     I"
     gene            complement(584..1168)
                     /locus_tag="PK610_11135"
     CDS             complement(584..1168)
                     /locus_tag="PK610_11135"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRJ36536.1"
                     /translation="MNPKIIKEALVYDDRIRVMKAELELLAIDGKTLHFDRSRVQRED
                     AAAVILFDRERDCFIFISQFRYPVAYHQEKVLMELVAGKVDEGEDPFVTAIRETEEES
                     GYVVPAERMKFMCTFFASPGYTTERYFLYLAEVTASDFTSKGGGLEEENEYIEKIEMT
                     REAFFRACLKGEITDGKTLMAGLWLMNSGFSKTH"
     gene            1258..2142
                     /locus_tag="PK610_11140"
     CDS             1258..2142
                     /locus_tag="PK610_11140"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HRJ36537.1"
                     /translation="MKTSPFAQYIEEVLKKHLGESIKLKGAEMAHGGSINECYILDTN
                     KGKYFIKRNDAVDFPGMFSEEAKGLNALRSTQTLRIPEVIEVGHFKSYAFLLLEYLEK
                     GTPSNAVWDKFGEQLALLHKHSNEHYGWSSENYIGSLTQNNTNYNSWAEFLIHTRLEP
                     QALLALTNKKIDRKLYDKLQQLYTRLDSVFPVELPAMLHGDLWSGNFLALASGEVAVF
                     DPAIYYGHREMDLAMTRLFGGFNQRFYDAYNAHYPLINDWQKRVDICQLYPLLVHVNL
                     FGGGYVHQVEMIVKKRTL"
     gene            complement(2167..>3305)
                     /locus_tag="PK610_11145"
     CDS             complement(2167..>3305)
                     /locus_tag="PK610_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36538.1"
                     /translation="LLVNENAKKVTAEFGIDYSAGGYSDSYFFMSVYSIYLEGAKVLL
                     PDSLSVLSWIGGDKFLVLSKGKNSTYAILDLSASGNLKLQALPENYTDLFAFENGNYG
                     YGEIKDNQFVYSIFNGASNERVAGRTTGQVTVYEADEFQSGFLLLFDSDSLHVLSGKG
                     DFLAHYGIQGIEEPHSIIGGRIHRFTTTTGTFFLVGGNKIVPASDYLIWPPNVTTGRI
                     LLVNRLPTAVTIHSISGTTLANVSCLFKGSEISGDGTQNVLEFQCKESAASIFYTNMQ
                     TNSWTMVAGAPVLNHYKFVLQNDYALYEYTVHLFLTVNNDLVLVDDRYREIRRWKGVK
                     NIKQILPDQQPEIIELEYVDSRIPNERINLFVLYPAKKQWKKKK"
BASE COUNT         1014 a          749 c          585 g          957 t
ORIGIN      
        1 aattgttaac tgatcactta acaatttaaa ctgagcggcc cgatctcccc gtttgcccaa
       61 atagccataa taatcgttta ctacttcaat aaaaacttcg acccctgcct tttgttcagc
      121 aagacgatat gcttctgaat acttttcaat cactttatca tgattattca acagttggta
      181 cgcttttgcc tgacgcacca gaagtccaac aatcatcttt tggttattac ttttatcata
      241 atagcgaaaa gccacttgaa aattctccag ggctttctca ggagatgaat tcttcaggta
      301 aacttcaccc ttattcactt ttgtaaacgc cgaaagagat ggtgattctt tcgagatcat
      361 ttccatctcg gtgataacag ataaaccgtc atccatgctt ttctttgctg ctttaactgc
      421 caattctgcc agtgcataaa ctttaactga atcactaccg gaagatgctt taatctcttc
      481 cattttttct ttcaaagtgt tttgtgcatg aacagtgccg gccaacacga aagctgttgc
      541 aataaagaac agcgaaattc ctattctgaa tggtgccttc atttcaatgc gttttgctaa
      601 atccggaatt catcaaccac aacccggcca tcaaagtctt gccatctgta atttcgcctt
      661 tcaaacaagc cctaaaaaat gcttctcgtg tcatttcaat tttttcgatg tattcattct
      721 cttcttccaa accacctcct tttgaagtaa aatcggaagc agttacctct gccaaataga
      781 gaaaatacct ttcggtggta tatcccggtg atgcgaaaaa agtacacata aacttcatcc
      841 gctctgcagg aacaacatac cccgactcct cctcggtttc acgaattgca gtaacaaagg
      901 gatcctcacc ttcgtcaact tttccggcaa ccaattccat tagcaccttc tcctgatgat
      961 aggcaacagg atacctgaat tgagaaataa aaataaaaca atcgcgctcc cgatcaaata
     1021 aaatcactgc agctgcatct tcacgctgaa ctctggaccg atcgaaatga agcgtcttac
     1081 catcgattgc aagcagttcc aactcagctt tcataacacg aatacgatca tcgtatacca
     1141 gtgcttcctt tatgattttt ggattcattc cccaatacta ctaaaagtca ggcctgtgcg
     1201 caagtattaa gggaaaccta ttcgtggaat tctacgtaaa agcaagccaa agctatcatg
     1261 aagaccagtc cctttgccca atacatcgag gaagtcctca aaaagcatct aggagagtcg
     1321 ataaaactca agggtgctga aatggcgcat ggtggaagca tcaatgagtg ttatattctt
     1381 gataccaaca agggtaaata ttttattaag cgcaacgatg ctgttgattt tccgggaatg
     1441 ttttcggagg aggcaaaagg attaaacgcg cttaggtcga cacaaacact tcgcattcct
     1501 gaagtaattg aagtcgggca tttcaaaagt tacgcatttc tcttattgga atatctcgaa
     1561 aagggaacac cttcaaatgc tgtgtgggat aagtttggcg aacaacttgc cttgcttcac
     1621 aaacattcca atgaacacta tggttggtca tcagaaaatt atattggatc cttaacccaa
     1681 aacaacacaa actacaactc ctgggcggaa ttcctaatcc atacccgatt ggaaccacaa
     1741 gccttattgg cacttaccaa caaaaaaatc gatcgaaaac tctacgataa acttcagcaa
     1801 ttgtacacca ggttggattc ggtttttcct gtagaattac ctgctatgct acatggtgat
     1861 ttgtggagtg gtaatttctt agcccttgca agcggcgaag ttgcggtgtt tgatcctgcc
     1921 atctattatg gtcaccggga aatggatctg gccatgacgc gtctatttgg gggttttaat
     1981 caacgtttct acgatgcata caatgcacat tatccgctga taaacgactg gcaaaaacgg
     2041 gtggatattt gtcagcttta tccacttctt gttcatgtca acctctttgg aggagggtat
     2101 gtccatcagg tggaaatgat cgtaaaaaaa cggacgcttt gattcctgat caaaaaagcc
     2161 ctcatttcat tttttcttct tccattgttt ttttgcagga tacaatacaa aaagattgat
     2221 ccgttcgttc ggaattcggg aatcgacata ttccagttcg ataatttccg gttgttgatc
     2281 tggaagtatc tgtttaatat ttttaacccc tttccaacgg cgaatctccc tataccgatc
     2341 atcaaccaac accaaatcgt tatttacagt caaaaataaa tgaacagtgt attcatacag
     2401 cgcataatca ttctgcagaa caaatttgta atgattcaat acaggcgctc cagctaccat
     2461 tgtccatgaa ttggtttgca tattggtata aaaaatactt gctgcagatt ctttacactg
     2521 aaactccaaa acattctgag tgccgtctcc tgagatttcc gatcccttaa acaaacagga
     2581 aacattagct agtgtagtcc cacttattga gtggatagtt acagcagttg gtaagcgatt
     2641 gaccaacaaa attcgaccgg tagtcacatt aggtggccag attaaataat cggatgccgg
     2701 aacaattttg tttccgccaa ccaaaaagaa ggtgccggtc gttgtagtaa agcgatgaat
     2761 tcttccaccg ataatagaat gcggctcttc aataccttgt attccataat gcgcaagaaa
     2821 atcaccttta ccagaaagaa catgaagact gtcagaatcg aataacagca aaaatcctga
     2881 ttgaaattca tctgcttcat aaaccgtaac ctgtcctgtc gttcgaccgg caacgcgttc
     2941 attagatgct ccattaaaaa tcgaataaac aaactgattg tcttttatct ccccataacc
     3001 gtaatttcca ttctcaaagg caaaaagatc cgtataattt tcgggaagcg cttgcagttt
     3061 cagatttcct gaggcggaaa gatccagaat ggcataagta ctgttttttc cctttgaaag
     3121 gaccaaaaat ttatcgccgc caatccaaga taatacactc aggctatcag gcagaagtac
     3181 tttagcgcct tccaggtaaa tagaataaac cgacataaaa aaataactat cagaatagcc
     3241 ccctgcggaa taatcaattc caaattcagc agttactttc ttagcatttt cattcactaa
     3301 caaat
//
LOCUS       DAOMYG010000372         3305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10544_length_3305_cov_7.413538, whole genome shotgun sequence.
ACCESSION   DAOMYG010000372 DAOMYG010000000
VERSION     DAOMYG010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10544_length_3305_cov_7.413538"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PK610_11150"
     CDS             <1..345
                     /locus_tag="PK610_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36539.1"
                     /translation="KYETTIKISGASYSRIIVDKDGIVGHDSRFVIGDNKDEAKKVLA
                     SMITLIKANMPPKFILRETWDDKFEDMAQVVEFDSDVFAQQAKQPSAKTGIRKIEGKF
                     VIDLTVYEPIFK"
     gene            460..2070
                     /locus_tag="PK610_11155"
     CDS             460..2070
                     /locus_tag="PK610_11155"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRJ36540.1"
                     /translation="MSQSAKYIFVTGGVASSLGKGIISASLAKLLQARGYSVTIQKLD
                     PYINVDPGTLNPYEHGECFVTEDGAETDLDLGHYERFLNVPTSQANNVTTGRIYLNVI
                     EKERKGEFLGKTVQIIPHITDEIKNRIKILGNSGKFEFVITEIGGTVGDIESLPYVEA
                     VRQLKWEMGGDCIAIHLTLIPYLAASGELKTKPTQNSVKQLLELGIQPDVLVCRTEHH
                     LNREIKEKVAKFCNVSKNAVIESIDASTIYEVPLLMKDEELDLVVLSKLNMPARTEPD
                     LTQWNDFLYHLKNPTRKVKIGLVGKYIELKDSYKSIVESFIHAGAANDCKVELVWLHS
                     ESLTDENAAEKLDGLRGILVAPGFGSRGIEGKIAAIRYARENKIPFFGICLGMQCAVI
                     EFARNVLGHKDAHTMEIEPTTKNPVIALMESQKGVTSKGGTMRLGAYPCKVAESSHTF
                     QAYGKSDISERHRHRYEFNNDYRDSMNKAGLQTVGINPDNDLVEIVELKDHPWFVGVQ
                     FHPEYKSTVVNPHPLFVAFVKATTDHNR"
     gene            2082..2486
                     /locus_tag="PK610_11160"
     CDS             2082..2486
                     /locus_tag="PK610_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36541.1"
                     /translation="MNNNDLIQIAEFQKEIGSYIGGLEPDSIVFEFSAVENKTRLDLV
                     TVNPRHRQSFLFHTVDGYDKVDALRKMLDYVRNYKERESSYTIQWSLRGETELHTSYF
                     RAKNIFDALDKLTYGRDLNSIIVYSVVLNPIS"
     gene            2489..2905
                     /locus_tag="PK610_11165"
     CDS             2489..2905
                     /locus_tag="PK610_11165"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRJ36542.1"
                     /translation="MIQPVRPNAHVCRMEVRFVDIDVMGHVNNAVYLNYFEQARMHFF
                     AAKIGTEWDWVNHGILLARNEVEYKQPTRLHEDVHIEVWIGKVGGKSLEMEYEVFVPS
                     GNDRILKAFGKSVLVCFDYKQQCTIPIPEQWRAAFV"
     gene            3090..>3305
                     /locus_tag="PK610_11170"
     CDS             3090..>3305
                     /locus_tag="PK610_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36543.1"
                     /translation="MVSLATLKRIITEMCAEGWLEKSGTGKKNTVYFLSSKSEVLWPV
                     ETADYFKKEIDERRIKREFDFAVVSGMF"
BASE COUNT          993 a          629 c          729 g          954 t
ORIGIN      
        1 aaatatgaaa ctaccattaa aatttcaggc gcaagctatt caagaattat tgtcgataaa
       61 gatggaattg ttggtcacga ttcacgattt gtaattggag acaataagga cgaagcgaag
      121 aaagtactgg catccatgat tactttgatc aaggcgaaca tgcctccgaa attcattctg
      181 cgcgaaacct gggatgataa atttgaggat atggctcaag tggttgaatt tgattcggat
      241 gtatttgctc aacaggcaaa acagccttct gccaagaccg gtatccgcaa aatagagggt
      301 aaatttgtga tcgatctaac ggtttacgag ccgattttca aataagaatt tcctgaattt
      361 tctcagtaaa aggcggaatt atccgccttt tttttgtttt caggcattcg gggggctttt
      421 tcccttaact ttgaaccccg tagttacatc acaaaaaaca tgtcgcaatc tgctaagtac
      481 attttcgtaa ccgggggtgt tgcttcttct ttagggaagg gtatcatttc agcctctctg
      541 gctaaacttc ttcaggcaag aggttactcc gttaccattc aaaaactcga tccctacatc
      601 aatgtggatc ccggaacatt aaatccttat gaacacggtg agtgtttcgt aacggaagat
      661 ggtgccgaaa ccgatctcga cctcggacat tacgaacgtt ttctgaatgt tccaacgagc
      721 caggccaaca acgttacaac gggacgcatt taccttaatg taattgagaa ggaacgcaag
      781 ggggaattcc tcggaaagac ggtgcagatc attcctcata ttaccgatga gatcaagaac
      841 aggatcaaga tcctgggtaa ctccggaaag tttgagtttg tcattacaga aatcggcgga
      901 acagtgggag atatcgaatc gcttccttat gtcgaagctg tacgtcagct gaaatgggaa
      961 atgggtggcg attgtattgc tattcacctt acgctgattc cttatctggc agcatccggt
     1021 gaattgaaaa ccaaaccaac ccagaattca gtcaagcaat tgctcgaact cggaattcag
     1081 ccggatgttt tggtatgccg taccgaacat catctcaaca gagagataaa ggaaaaagtt
     1141 gcgaaattct gtaacgtcag caaaaatgca gttatcgaat ccatcgatgc atctaccatt
     1201 tatgaagtgc cgctactcat gaaggatgag gaattggatc tggtggtgct ttcaaaactg
     1261 aatatgcctg cccgaacaga accggatctt acacagtgga atgattttct ctaccatttg
     1321 aaaaatccta cacgtaaagt gaaaattggc ttggttggaa aatacatcga gctaaaggat
     1381 agttataaat caattgttga atcctttatc cacgcaggtg ctgcaaacga ttgtaaagtg
     1441 gagttggtct ggttgcattc cgaatccctt accgacgaaa atgctgcaga aaaactcgac
     1501 ggactacgcg gaattttagt cgcaccggga tttggttcga gaggaataga aggaaaaatt
     1561 gctgcgatcc gttatgctcg tgaaaacaaa attccctttt tcggaatctg tttagggatg
     1621 caatgcgcag tgattgaatt cgccagaaat gtgctcggtc ataaagatgc acataccatg
     1681 gagattgaac ccactacaaa aaatccggtt atcgcattaa tggaatcgca aaaaggagtc
     1741 acttccaaag gaggaaccat gcgattgggt gcatatccct gtaaggttgc tgaatcttca
     1801 catacattcc aggcttacgg aaaatcagat atttctgaac gtcaccgcca tcgttatgaa
     1861 ttcaacaacg attaccgcga ttcaatgaac aaagccggat tgcaaacagt aggaatcaat
     1921 ccggataatg atctggttga aattgttgaa ctcaaagatc atccttggtt tgtgggtgta
     1981 cagttccacc cggaatacaa aagtaccgtg gtaaatcctc atccgctttt cgttgctttt
     2041 gtgaaagcta ctaccgatca taaccgctga ttttttctgc catgaacaat aatgatctta
     2101 ttcagattgc agaattccaa aaggaaatag gttcgtatat cggcggactc gaacctgata
     2161 gtattgtgtt tgaattctct gctgtagaaa ataaaacacg actcgatctt gtgactgtaa
     2221 acccgcgtca ccgtcagtca tttttgtttc atacagtaga tggttacgat aaagtcgatg
     2281 ccttgcgcaa aatgctcgac tatgtccgga actacaaaga gcgcgaaagt tcctatacca
     2341 ttcagtggtc gctacgtggt gaaacggagt tgcatacttc ctatttccgt gcaaaaaata
     2401 tttttgatgc attggataaa cttacctacg gacgcgatct caactcgatc attgtttaca
     2461 gtgtagttct caatccaatt tcctgaagat gattcaacct gttcgaccca atgcacatgt
     2521 ttgcagaatg gaagtccgtt ttgtggatat tgatgtaatg ggacatgtaa acaatgctgt
     2581 ttacctgaac tattttgagc aggcgcgtat gcattttttt gctgccaaaa ttggtacgga
     2641 gtgggattgg gtcaatcatg gaattttgct tgcacgcaat gaagtggaat acaaacaacc
     2701 cacacgtttg cacgaagatg tacatataga agtttggatc ggtaaagtgg gaggcaagag
     2761 tcttgaaatg gaatacgaag tatttgtccc ttccggaaat gatcgtattc tgaaagcttt
     2821 tggtaaaagt gttttggttt gttttgatta caaacaacaa tgcaccatcc ctattcctga
     2881 gcaatggcgt gctgcttttg tttgatttta tagctcattt ttggtgtttt tgtagctcaa
     2941 aacgatagtt tttgagcttt atttttacta tttttgagct ataaatagtt aagttgagag
     3001 ctcgaaaaca agttaaaaat ttaataatca gtattttatc tgataaggag cggagatcct
     3061 caggtgagtt attttctgaa ttgtccggaa tggtatcact cgctacccta aagcgaatta
     3121 ttacagagat gtgtgcagaa ggttggctgg aaaaatcagg aacaggaaaa aagaatacgg
     3181 tttattttct atcctcaaaa tctgaagtat tatggcctgt tgaaacagcc gattatttta
     3241 agaaagaaat tgatgagcgg agaataaaac gcgaatttga ttttgctgtc gtatccggaa
     3301 tgttt
//
LOCUS       DAOMYG010000373         3304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10550_length_3304_cov_6.638350, whole genome shotgun sequence.
ACCESSION   DAOMYG010000373 DAOMYG010000000
VERSION     DAOMYG010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10550_length_3304_cov_6.638350"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..686
                     /locus_tag="PK610_11175"
     CDS             99..686
                     /locus_tag="PK610_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HRJ36544.1"
                     /translation="MLSHLQSYNIFLASKSPRRQALLKGLDIEFAIRTKETDEEFPEH
                     LKADEIVLYLSKKKADAFSGELQEKDILITADTIVWINNRVLNKPENRTEAIEMLKLL
                     SGKKHTVYTGVCITASSFQTQFVGSTQVWFKELTLAEIDYYLDHYKPYDKAGSYGVQE
                     WIGYIGIDRIEGCFYNVMGLPLNLLYAHLREIPLH"
     gene            703..1263
                     /locus_tag="PK610_11180"
     CDS             703..1263
                     /locus_tag="PK610_11180"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HRJ36545.1"
                     /translation="MLFFTDHLHGQSYADSLEQYRVNRNKEMKDTAHSPLKKEEILKF
                     KELHFYPANSDYRIYAAFIKNRRKETVTIPTSSGKEKQYYKIGKAAFELNGVACTLSV
                     FQPVALKNYIFIPFTDLTNSDETYGGGRYLEASVIGKKKILIDFNYCYNPYCAYTSGY
                     SCPIPPTENFLNTAVKAGEKLLWDSH"
     gene            1297..2769
                     /gene="nhaC"
                     /locus_tag="PK610_11185"
     CDS             1297..2769
                     /gene="nhaC"
                     /locus_tag="PK610_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HRJ36546.1"
                     /translation="MSAPKNTPVPGLFISFLPILVLVGFLTINVKLYGDSATSGPNQI
                     ALILTAAFAGIIAWRYGLTWTRIRDGIMHSIIASLPAVMILLLIGALAGTWMISGVVP
                     TMIYYGLKILHPAIFLLAATIVCAIVSLATGSSWSTVATVGAALLGIGSAMGFHEGMI
                     AGAIISGAYFGDKMSPLSDTTNLAPAMAGTDLFTHIRYMTITTVPSLAITLLIFLVLG
                     FTAELNPVAGGVDSLMSAIDSKFYVSPVLFLVPSVVIFLIIKKVDAVPALLIGTLLGG
                     IFAVIFQPEVVNEISGITSDYVKSGYSTIMNAMMGPVSVHTSDDQINSLLSTKGMGGM
                     LNTIWLIICAMSFGGIMEVSGMLQKISSTVVNLARSTGSLIASTAGTCIFFNATAADQ
                     YLSIVVPGRMYAKTYRDRGLKPENLSRTLEDSGTVTSVLFPWNTCGATQAGVLGVATG
                     TYWMYCFFNLISPIMTIAAGYLNYRIARYSEEEMKAMNQE"
     gene            complement(2760..>3304)
                     /locus_tag="PK610_11190"
     CDS             complement(2760..>3304)
                     /locus_tag="PK610_11190"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HRJ36547.1"
                     /translation="SYPAPGHPKLAAEIISTVKSTAILPDEQWGLDHGTWSILLPMFP
                     KADIPVIQLSLDRSKGPSYHFELAKELRSLRKKGVLIIGSGNIVHNLGRILWEDKAYD
                     WAIEFDSKIKTAIDANDSKTLIDYTSLGKAALLSVPSNEHYLPLLYAAGLRDEKDKVS
                     YLNDKTTMGAISMRSVMYHS"
BASE COUNT          927 a          638 c          675 g         1064 t
ORIGIN      
        1 acctcatcaa caaacgacag aaatggattt ctcggagctg ctaaatggat gaaatttgcc
       61 atgtttgccg gcgtgttgta tagcctattc ctgttctgat gctgagtcac ctccaatcct
      121 acaacatttt ccttgcctca aaatctccac ggcgacaagc tcttttaaaa gggctcgaca
      181 tcgaatttgc tatccgaacc aaagaaaccg atgaagaatt cccggagcat ttaaaagcag
      241 atgaaattgt actgtattta agtaaaaaga aagcggatgc tttttccgga gagcttcagg
      301 aaaaggatat tttgataacc gcagatacaa ttgtttggat caataaccgg gttttaaaca
      361 aacccgaaaa cagaacggag gcaattgaaa tgctcaaatt gctttccgga aagaaacaca
      421 ctgtttatac aggagtctgc ataacggcaa gctcattcca aactcaattt gtaggttcaa
      481 cgcaggtgtg gtttaaagag ttgactcttg ctgaaattga ttattacctc gatcattata
      541 aaccctacga taaggcaggt tcatatggag tccaggagtg gattggttat ataggtatag
      601 atcgtataga aggctgtttt tataatgtaa tgggtttacc acttaatctt ctttatgcac
      661 atttacgtga aattcctttg cattagtttt ttcctaggag ggatgctttt tttcactgat
      721 catcttcatg ggcagtccta tgcagattct ctggaacaat accgtgtaaa ccgtaacaag
      781 gagatgaaag ataccgcaca ttctcccttg aaaaaggaag agatcctgaa atttaaagaa
      841 ctacattttt atcctgcaaa ttccgactac agaatttacg ctgcctttat aaaaaatagg
      901 cggaaagaaa cagttaccat acctacctcc tcaggtaagg aaaaacaata ctacaaaata
      961 ggaaaggctg cttttgaatt gaacggcgtg gcctgtacac tttctgtttt tcagcctgtg
     1021 gcattaaaga attatatttt tattccgttt acagatctaa ccaatagtga tgaaacatat
     1081 ggtggtggca gatatctgga agcatctgtt attggaaaaa agaaaattct gatcgatttt
     1141 aattattgct acaatcctta ttgcgcctat acttccggtt attcctgtcc aataccaccg
     1201 accgagaatt ttttgaatac agcagtcaaa gccggtgaaa aattactgtg ggattcgcat
     1261 taattctgtt tcagtatatt tatccaaata ttttgcatgt ctgctccaaa aaatacacct
     1321 gttccgggat tgttcatttc ctttttaccc attcttgtac ttgtaggttt ccttacaata
     1381 aacgtaaaat tgtatggcga cagcgcaacc tctggcccta atcagatcgc gctaattcta
     1441 accgcagctt ttgcgggaat cattgcctgg agatatgggt taacctggac ccgcattcgc
     1501 gatggtatca tgcatagtat cattgcttct ttgccggccg ttatgatttt attgttgatc
     1561 ggtgcacttg caggaacatg gatgatttcg ggtgtagttc ctactatgat ttattatgga
     1621 ttaaaaattc ttcatcctgc tatttttctc cttgcagcaa ctattgtttg tgcaattgtt
     1681 tctcttgcta ccggaagttc ttggtctact gttgcaactg ttggtgctgc tttgcttgga
     1741 attggttctg ccatggggtt tcatgagggc atgatagcag gtgcaattat ttcgggagcc
     1801 tattttggag acaagatgtc gcctttatcg gataccacta atcttgctcc tgcaatggca
     1861 ggtacagatc tgttcacgca tattcgttat atgacaatta ctactgttcc atcattggca
     1921 attactcttt taattttttt ggtgcttgga tttactgctg aattaaatcc ggttgccggt
     1981 ggggttgatt cattgatgtc ggccatcgat tcaaaatttt atgtttcacc ggttttgttt
     2041 ttggttccat ctgttgtcat ttttctaatc ataaaaaaag ttgacgcagt tccggctttg
     2101 ctgattggta ctttattggg cggaatcttt gcggttattt tccaaccgga agtagtaaat
     2161 gaaatttctg gaattacaag tgattatgta aagtcaggtt actcaacaat tatgaatgct
     2221 atgatgggac cggttagtgt gcatacttcc gatgatcaga tcaattcact tttgtcgacc
     2281 aaaggaatgg gagggatgtt gaatacaata tggctgatta tttgcgcaat gagttttgga
     2341 ggaataatgg aagtaagcgg aatgctgcaa aaaatttcgt caacagtagt aaatcttgcc
     2401 cgttcaacag gatcattgat cgcttctact gccggtactt gtattttttt caacgcaact
     2461 gcagcagatc aatatttgtc gattgttgta cccggcagaa tgtatgcaaa aacatatcgc
     2521 gatcgtggtt taaaaccgga gaatttaagt cgcactttgg aagattccgg aacagttact
     2581 tctgttcttt tcccctggaa tacatgtgga gcaactcagg ccggagtatt gggcgttgca
     2641 accggaacat actggatgta ttgcttcttt aatttgatta gtccaattat gacaatcgca
     2701 gcaggatatt tgaattaccg gattgcccgc tattctgaag aagaaatgaa agcaatgaat
     2761 caggaatgat acattactga ccgcatgctg attgcaccca ttgtggtttt gtcgttcaag
     2821 taactgacct tatccttttc atctcttaat ccggccgcat agagtaaagg taagtaatgt
     2881 tcgtttgaag gcacagataa aagcgctgcc tttccaaggg atgtataatc aatcagcgtt
     2941 tttgaatcat tggcatcaat cgccgtttta atttttgaat caaactcaat tgcccaatca
     3001 taggctttgt cttcccacaa aattcttccc agattgtgta caatatttcc gctaccaata
     3061 atcaacacac ctttttttcg aagtgagcga agttcttttg caagttcaaa atgataagac
     3121 ggacctttac ttcgatccag actcaattga ataaccggta tatcagcctt tggaaacatg
     3181 ggcaaaagta tcgaccatgt accatgatca agtccccatt gttcatccgg aagaattgct
     3241 gttgatttaa ctgtactgat aatttctgct gccagttttg gatgacctgg agcaggatag
     3301 cttt
//
LOCUS       DAOMYG010000374         3303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10555_length_3303_cov_7.920567, whole genome shotgun sequence.
ACCESSION   DAOMYG010000374 DAOMYG010000000
VERSION     DAOMYG010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10555_length_3303_cov_7.920567"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PK610_11195"
     CDS             complement(<1..662)
                     /locus_tag="PK610_11195"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ36548.1"
                     /translation="MKILQLCNKPPKPAIDGGCIAMNNITQGLISEGHQVKILTIETQ
                     KHPALFEKMSAEYIANTQIEAVYIDTSINLIDAFSALVTSDSYNVSRFFSPDFDRKLI
                     EVLTKDNYDIIHLESLFMTPYIATIRRYTKSPVVLRSHNLEYIIWERLADVSKNKAKK
                     AYLKLLSRQLKKYETGIINSVDGIAAISNEDARKYLGLKCFKPIVNIPFGIDIENYPK
                     ES"
     gene            complement(673..2178)
                     /locus_tag="PK610_11200"
     CDS             complement(673..2178)
                     /locus_tag="PK610_11200"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRJ36549.1"
                     /translation="MGKLTRLVIASYLGPFILTFAIAMVFLILQFLWKYVDDLMGKGL
                     EWYVVAELLFYASANLVPMALPLAILLSSIMVFGNLGESSELTAMRSAGLSLMRIMRP
                     LIIFIAFISISAFFFANYLWPVANMKFRSLIFDIQNQKPALTLTERRFYNDIPGFSIR
                     VMEKTKEGSHFKDILIYDHSTGNVNFKRDIWAESGNLEKINGGKGMLFHLNNGKIYEE
                     VQGRMMKGGIFPYQKIQFKKASLYFDLSSFQLQRTDVQVFNTHYEMMNMAQLASAEDS
                     LRMNLYEKRKEMSDNFARRFLIFRPVGNFDPTHPVAVPTLTLNKVANQKPILKSANDS
                     IQPSSVNHSFVPFREQHGEQRTRTLASAQSMARNSLEYILGRKEEFTGREEFIQRHRL
                     EWHRKLTLSVACFILFFIGAPLGAIIRKGGLGTPIVASTILFIFYYILSISGEKMAKA
                     NVLDPFWGMWMSSFILFPVGLYLTWKSNNDSALFDKDFYLRLFWIKRLRKS"
     gene            complement(2220..2606)
                     /locus_tag="PK610_11205"
     CDS             complement(2220..2606)
                     /locus_tag="PK610_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HRJ36550.1"
                     /translation="MSSKVKFELEFPLRSSNKVLFNSVSTPDGLSEWFADDVNIRNDI
                     FIFRWDDTEERARLISKKPFEHVRFQWDREDEEENTMFEFHIKTDPLTKEVALMIVDH
                     AEENDVEDSTFLWNNQINVLRQRLGA"
     gene            2718..>3303
                     /locus_tag="PK610_11210"
     CDS             2718..>3303
                     /locus_tag="PK610_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRJ36551.1"
                     /translation="MLNGKRIIVVLPAYNASQTLERTYNEIPFDIVDDVVLVDDASKD
                     DTSEVGRKLGIKHVIRHENNRGYGGNQKSCYNKALELGGDIIVMLHPDYQYTPKLIHA
                     MASVIAYDVYPVTLGSRILGKGALKGGMPKYKYIANRFLTLGQNILMGQKLSEYHTGY
                     RAFSKEVFTRINIEANSDDFVFDNQMLAQIFFAGF"
BASE COUNT          984 a          674 c          670 g          975 t
ORIGIN      
        1 tctgattctt ttggataatt ttcaatatcg attccgaacg gaatatttac tatgggtttg
       61 aagcacttta atccaagata ctttcgggca tcttcattgc tgatcgctgc aattccatct
      121 accgaattaa tgattccggt ttcgtatttt ttgagctggc gtgagagtag tttgaggtac
      181 gctttttttg ctttattctt cgatacatca gcaagtcgct cccaaataat atactccaga
      241 ttatgcgatc gtaataccac tggcgatttt gtatatcgtc tgatcgttgc gatatatgga
      301 gtcataaata aactctcgag gtggatgatg tcgtaattgt cttttgttaa aacctcaatc
      361 agttttctgt caaaatcagg agaaaaaaaa cggctgacgt tgtacgaatc actggtaacc
      421 aatgcagaaa atgcatcaat cagattaatg gaagtatcaa tgtaaaccgc ttcaatttga
      481 gtgttggcga tatattctgc cgacattttt tcaaaaagcg cggggtgttt ttgcgtttct
      541 attgtaagaa tttttacctg atggccttct gaaatcaatc cttgcgtaat attattcatt
      601 gcaatacaac ctccgtcgat ggcgggcttg ggtggtttat tgcataactg aaggatcttc
      661 atagaggcaa aattacgatt ttcttaatcg ttttatccag aataagcgga ggtaaaagtc
      721 tttgtcgaac aaggcagaat cgttatttga tttccaggtt aggtaaagtc caaccggaaa
      781 taaaataaaa gagctcatcc acattcccca aaagggatcc aaaacattgg ctttcgccat
      841 tttttctccg gaaatggaaa ggatgtaata gaaaataaac aaaatcgtag aagcaacaat
      901 tggtgtacct agtcctcctt tgcgaataat tgctcccaac ggcgccccaa taaagaatag
      961 tatgaaacaa gccaccgaca atgtcagttt gcggtgccat tccaaacggt ggcgctgaat
     1021 gaattcttct cttccggtaa attcttcctt tcgtcccaat atatattcca aagaattcct
     1081 tgccatcgat tgagcgcttg ccaaagtacg ggttcgttgt tccccatgtt gttcgcgaaa
     1141 gggaacaaag gaatggttta ctgaagaagg ttgaatagaa tcgttggccg atttcagaat
     1201 aggtttttga tttgctactt tattcaatgt taatgttgga actgccaccg gatgagtggg
     1261 gtcaaaattt ccgaccggac gaaaaatgag aaaacgacgt gcgaaattat cactcatttc
     1321 cttgcgcttt tcatacaaat tcattctcag cgaatcttct gcagaggcaa gttgcgccat
     1381 attcatcatc tcatagtgcg tattaaacac ctgaacatct gttcgctgca attgaaatga
     1441 actgagatcg aaatacaaac tcgctttttt aaattgaatt ttctggtaag gaaaaatccc
     1501 ccctttcatc attcttccct gaacttcttc atagattttt ccattgttaa gatggaacaa
     1561 cattcccttt cctccattga tcttttcgag gtttccggac tctgcccaga tatctctctt
     1621 gaaatttaca tttccggtgg aatgatcgta aatcagaata tccttaaaat gacttccctc
     1681 cttggttttt tccattacac ggatactgaa acctgggata tcattgtaaa atcttctttc
     1741 tgtaagtgta agtgcaggtt tttggttttg aatatcgaaa atgagcgacc tgaatttcat
     1801 gtttgcaaca ggccataagt agttggcaaa aaagaaagcg gagatactga tgaatgcgat
     1861 aaaaatgatg agtggtcgca taatgcgcat caatgataat cctgcagacc gcatggccgt
     1921 gagctcgctg ctttcgccaa gatttccaaa taccatgatc gaactaagca gaatggctag
     1981 tgggagtgcc ataggaacca gatttgctga ggcatagaac agcaattctg caacaacata
     2041 ccattccaaa cctttcccca taaggtcatc cacatacttc cacaaaaact gaaggataag
     2101 gaagaccata gcaatagcaa aggtcagaat gaacggaccc agataggaag caatgacaag
     2161 acgtgtaagt ttccccactg tgcaattaac gctgcaattc tacgaaaatt gtaggagctt
     2221 caggctccca ggcgctgacg aaggacgttg atctggttgt tccataagaa tgtggaatcc
     2281 tccacatcat tttcttccgc atgatcaaca atcattaacg ctacctcttt ggtaagtggg
     2341 tcggtcttaa tgtgaaattc gaacatagta ttctcttctt catcctcacg gtcccactga
     2401 aagcggacat gctcaaatgg ctttttgctg atcaaccggg cacgctcctc ggtatcgtcc
     2461 cagcgaaaaa tgaaaatgtc attgcggatg ttgacatcat ctgcaaacca ttccgacaaa
     2521 ccgtcgggag tacttacgga gttaaataac actttattgg aggaccggag tgggaactcc
     2581 agttcgaatt tgactttact gctcattctg atggatttgc acctaatata ataggaaata
     2641 tcaagataaa caagaagtta attgttaaca cctgttggta tctcttgttt tttcacaagg
     2701 aaatgtactt ttgtgccatg ctcaacggaa aacgcataat tgtagttctg ccagcctaca
     2761 acgcatcgca gacacttgaa agaacctata acgagattcc attcgatatt gtggatgatg
     2821 ttgtccttgt ggatgacgcc agcaaggatg atacctccga agttggacgt aaactgggaa
     2881 ttaaacatgt cattcgacac gaaaacaacc ggggttatgg cggtaatcag aaatcatgct
     2941 acaataaagc attggagctt ggtggtgata ttattgtgat gttgcacccc gattaccaat
     3001 acacaccaaa gttgatacat gcaatggcaa gtgtaattgc atatgatgta tacccggtta
     3061 cgcttggatc ccggattctc ggaaaaggag ccctaaaagg aggaatgccc aaatacaaat
     3121 acattgccaa ccgttttctt acacttggtc agaatatcct catgggacaa aaactttccg
     3181 agtaccatac aggatacaga gccttttcaa aagaggtttt taccaggatc aatattgaag
     3241 caaattctga tgattttgta tttgataacc aaatgctcgc gcagattttt ttcgcaggat
     3301 ttg
//
LOCUS       DAOMYG010000375         3297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10582_length_3297_cov_8.969155, whole genome shotgun sequence.
ACCESSION   DAOMYG010000375 DAOMYG010000000
VERSION     DAOMYG010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10582_length_3297_cov_8.969155"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1065)
                     /gene="dapA"
                     /locus_tag="PK610_11215"
     CDS             complement(172..1065)
                     /gene="dapA"
                     /locus_tag="PK610_11215"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRJ36552.1"
                     /translation="MNKKFYGMGVAVVTPFNKSGEIDYAALQRILEHLINNGTDYLVI
                     QGTTGESVTLTEDEKRAVLDFAIEINNKRLPIVFGVGGNDTRDIIHTLQNFDFTGVDA
                     ILSVSPYYNKPTQEGLYQHYLQVNAHTPRPVILYNVPGRTGRNMTAETTLRVARDCKN
                     MIAIKEASGSFNQIMSIIQEKPKDFLVICGDDAIALPACSVGAVGVISVVGNAFPKEF
                     SEMIRLALQEKYSDAQSIHYILLDIIQHLFVESNPSGVKEVLKFLGLADNYTRLPIVP
                     VTDETSKKIYSLLAACPYVKI"
     gene            complement(1062..1613)
                     /locus_tag="PK610_11220"
     CDS             complement(1062..1613)
                     /locus_tag="PK610_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36553.1"
                     /translation="MMFEGIRKKVGRRQMRSEYKGLRRTPKVFNLATAQSVGLIYRVD
                     NEEKFAAVKKYVRHLKEEEGIKRIMALGYVEDKVLPEFARSQLEFDYFCKAELSWNFR
                     PGGNAVKNFCNEVFDILIDLEREEIIPLRHILNKSKGRFKVGYYTDEFKQYYDLMIDV
                     HSSEMIDYIAQVNYYLSIINKVS"
     gene            complement(1610..>3297)
                     /gene="ligA"
                     /locus_tag="PK610_11225"
     CDS             complement(1610..>3297)
                     /gene="ligA"
                     /locus_tag="PK610_11225"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRJ36554.1"
                     /translation="ELKYDGVAISITYENGKMVRAVTRGDGEQGEDITPNIKTIRTIP
                     LRLQQSDYPARFEIRGEIFFPRKNFEKLNREREAAGEPLFANPRNTASGTLKLQDSSI
                     VAKRGLDCFLYAVAGDALPFANHFESIQQAGEWGLKVPSEKENYIRRCSDIDAIMEFI
                     DYWDAARKELWFDIDGVVIKVNNYRQQQELGFTAKSPRWAVAYKFKAEQVSTLIESVS
                     YQVGRTGAITPVANLKPVLLAGTVVKRASLHNADQIEKLGLHHGDTVFVEKGGEIIPK
                     IVGIDLSKRDEHSGVFRFPDACPECGTQLYRNEGEAQHYCPNEMGCPPQIKGKLVHFT
                     GRKAMDIEGLGEETIEQLFAAGLIITIADIYDLRKEQLLPLERMAAKSVNNLLSGIEA
                     SKSVPFERVLFSMGIRFVGETVAKKLARHFRNIDAIAAASVEELVAVEEIGDKIALSV
                     YEWFRLPANLQLIDRLRSAGVQLELDPSQIKEKGDRLKGLSFVVSGVFKNYSRDGIKE
                     FIENNGGKVSGSVSKKTGYLLAGDEAGPSKLTKAQELGVKIISEEEFDKLLAE"
BASE COUNT          938 a          776 c          636 g          947 t
ORIGIN      
        1 tgttcacaag gggcgcgagc ccgagagctc tgacgagttg tggaacatcg atcgttttat
       61 ttttcacagg tgtttcatcc ggtaaactca ccattaatgg caataaaagt atgagtgcac
      121 ccgccaccag cagaaaaata aaactattgc ggttgtcaga aacgaagctc atcagatctt
      181 aacgtaagga caagcagcca gcagggaata aatctttttg ctggtttcat cggtaacagg
      241 aacaatcggt aagcgggtat agttatctgc aagtccgaga aacttcagca cctcttttac
      301 accggaagga ttgctttcta caaacaaatg ctgaatgata tccagaagaa tatagtggat
      361 cgactgtgca tcggaatatt tttcctgcaa ggcaagccgg atcatttcag aaaactcctt
      421 gggaaatgca tttcctacaa ctgaaatcac acccacggct ccaaccgaac aagcaggcaa
      481 cgcaatggca tcgtcgccgc agatcaccag aaaatctttt ggcttttcct ggataataga
      541 catgatctga ttaaaagaac cggatgcttc tttgattgca atcatgtttt tgcaatcgcg
      601 ggcaacacgt aaagtcgttt cggccgtcat atttcttcct gtacgtcccg gaacattgta
      661 caaaataaca ggacgtggtg tatgagcatt tacctgaaga taatgttggt acaaaccctc
      721 ctgagttggt ttattgtaat aaggactcac cgacaaaatc gcatcaacgc cggtaaaatc
      781 aaaattctga agtgtgtgaa taatatcgcg ggtatcgttt ccgccaacac caaatacgat
      841 tggcaagcgt ttgttattga tctcaattgc aaaatcaagc acagcgcgtt tttcatcttc
      901 ggttaaggtt accgattcac cggttgttcc ttgtataacc agataatcgg tgccattgtt
      961 gatgagatgc tcaagaatac gctgcaatgc tgcataatct atttcacccg atttattgaa
     1021 aggcgtgaca accgccactc ccattccgta gaatttttta ttcatgatac cttattgata
     1081 atagagaggt aatagttgac ctgagcaata taatcgatca tttccgatga gtgaacgtcg
     1141 atcataagat catagtactg cttaaattca tctgtgtaat aacctacttt aaaacgtcct
     1201 tttgatttat ttaaaatatg ccgaagcgga ataatctctt cacgttcaag atcgatcagg
     1261 atatcaaaga cttcattgca aaaatttttg actgcattac cacccggacg aaaattccaa
     1321 ctgagttctg ctttacagaa ataatcgaac tccaattgtg aacgtgcaaa ttcgggcaat
     1381 accttatctt caacatagcc caaagccata attcgtttta ttccttcttc ttctttgagg
     1441 tggcgcacgt atttttttac tgcagcaaac ttctcttcat tatcgacacg atagatgagc
     1501 ccgaccgatt gtgcagtagc cagattaaat actttaggtg tgcgacgaag tcctttgtat
     1561 tcggacctca tttgtcgtct gcccactttt ttacggattc cttcgaacat cattctgcca
     1621 acaatttatc gaattcttcc tccgaaatga ttttcactcc gagttcctgc gcctttgtca
     1681 atttacttgg tccggcttca tcacctgcaa gcagatatcc ggttttcttc gaaacagacc
     1741 cactcacttt tcctccgttg ttttcaatga attccttaat tccatcacgc gaatagtttt
     1801 taaatactcc ggaaacaacg aatgaaagtc cttttaagcg atctcctttc tcctttatct
     1861 gcgacgggtc caactccaac tgaacaccag ccgaacgtaa acgatcaatc aactgcaaat
     1921 tagccggaag cctgaaccat tcataaacac tcagggcaat tttatcccca atttcttcaa
     1981 cagccacaag ttcttctaca gaagcggcag caatagcatc aatattccga aagtgcctgg
     2041 ccagcttttt agctacagtt tccccaacaa aacggatacc cattgaaaac aacacccttt
     2101 cgaaaggaac ggatttggag gcttcaattc ccgacaggag gttattgacc gacttcgcag
     2161 ccattcgttc cagaggaagc aattgttctt tccggagatc ataaatatct gcaattgtaa
     2221 taatgagtcc ggctgcaaac agttgttcga tggtttcctc tccaaggcct tcaatatcca
     2281 ttgccttacg tcctgtaaaa tgcaccagtt ttccttttat ctgaggcgga caccccattt
     2341 cattagggca gtaatgttgt gcttctcctt cgttgcggta aagttgagtt ccacattcag
     2401 gacaagcatc cggaaacctg aatactccgg aatgttcatc gcgtttgctg aggtcaatac
     2461 caacaatttt tggaatgatc tctcctccct tttcaacgaa aacagtatca ccgtgatgca
     2521 atcccaattt ttcaatttga tcagcattat gaagagacgc acgttttaca acagtaccag
     2581 caagaaggac aggtttcagg tttgcaacgg gagtgatagc gcctgtgcga ccaacctgat
     2641 acgacacgct ttcgatcaag gttgaaacct gttcagcctt gaacttgtag gcaactgccc
     2701 aacgcggtga ttttgcagta aaaccaagtt cctgttgctg gcggtaatta ttcaccttta
     2761 tgacaacccc atcaatatca aaccacagtt ccttacgagc agcatcccag tagtcgataa
     2821 attccattat ggcatcgata tcggagcaac gtctgatata attctctttt tcagaaggca
     2881 ctttaagtcc ccattcgccg gcttgttgaa tactttcaaa atgattggca aaaggaagcg
     2941 catcacctgc aacagcgtag aggaaacaat ccaatccacg ttttgccacg atggaagaat
     3001 cctgcagttt aagtgttccg gatgcagtgt ttcgtggatt tgcaaataag ggttcaccgg
     3061 cagcctcacg ttcgcgattc aatttctcga aatttttacg cggaaaaaag atctcccctc
     3121 taatttcaaa tcgagcggga taatcgctct gttgcaaacg aaggggaata gtgcgaatgg
     3181 tttttatatt gggagtaata tcctctccct gctcaccatc tccacgcgta actgcacgaa
     3241 ccatttttcc attttcatag gttatggaaa ttgcaactcc gtcgtatttg agttcac
//
LOCUS       DAOMYG010000376         3272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10696_length_3272_cov_6.992850, whole genome shotgun sequence.
ACCESSION   DAOMYG010000376 DAOMYG010000000
VERSION     DAOMYG010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10696_length_3272_cov_6.992850"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="PK610_11230"
     CDS             <1..175
                     /locus_tag="PK610_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRJ36555.1"
                     /translation="DETGYLKVVPGTTKTNIPGVFAAGDCADKVYRQAVTAAGTGCMA
                     ALDAERYLAAKGH"
     gene            complement(245..1120)
                     /locus_tag="PK610_11235"
     CDS             complement(245..1120)
                     /locus_tag="PK610_11235"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ36556.1"
                     /translation="MKKLDANKKSRMMRYAAVAGAIGAAGSANAQILYTDITDQTVTP
                     GNSYLLNLDNATGDDYVITCSSRSGSLGGFPYTGVGVVINTFGSNGVASASSSQGIFP
                     LAAKLNANDAINNTLGFAQAPTSVSSSAMMLGAVANVTGLGTFPLGDWLGASNKYLGL
                     ALDVSGSLHYGWARLDVNINGNSFTIKDYAVHQTAGSTILAGQQTVGVEELNVRDKVD
                     MNINNKNLKINVLDPSLTNGVVTVTDISGKVVATFHLNGSNNSFDVSQLASGIYAINV
                     VFDQGVAKEKLFINN"
     gene            complement(1192..>3272)
                     /locus_tag="PK610_11240"
     CDS             complement(1192..>3272)
                     /locus_tag="PK610_11240"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ36557.1"
                     /translation="INQFSNSPDPENWLMAPGTVHPENIAEQLSQFRVHPAEITPAMV
                     LPFVPELYQMPEEESKVITTLRNEIAKAASLQAASTWLMQNTEWDVMGVYFDMIDHLS
                     HMAMKFRAPKLDGVPDEKFIFYNQVVDGAYRFQDMMLDRMLDLIDDNTAILILSDHGF
                     ESGNLRPRVIPVEPAGPTSEHSPYGMIAFSGPGIYSNTEIFGSSVIDITPTILHYMGL
                     PVGMDMYGKVLQQCFSEQKEIHWIDSWENHSDGGCSSGMHPEELRVNTWDSREALEQL
                     IELGYIERPEGTNEEIVAKTKAEVEYYLAQSLSFQKRDEEAAKILERNIPLYPEFHRY
                     RSLIIQCYIRLRKWKEAEMHTDALFGINTVLDLKVWFFRGKMAQAQYRPKKALFSYEK
                     ALEIAPSSIDIRLQKGNILLSMGQYREAENVFSEIANDDPRNAEANFGRGMALLRSGE
                     ADTAIDFFIDTLELKPRFAAAHFQLGECFRLCKDYRPAETAYLNALHIHPGMLRARNR
                     LMELYTTRIPDPEKLKEQVDWLAKFQKFTIKCVSGLPAAGQEELCEFLKSKGIECIDL
                     RPSIDSPELKADLNIKSINQNPEAIFLLTQAQLMKIPEDRPLKLIWLDSDDSLLFPAQ
                     LKRAGQESKIVNKVYPTHLATLNTEQRRVFGEWASVRPLLEIVHSDWLIENEAVEIQV
                     SGLFELSDNP"
BASE COUNT          963 a          704 c          634 g          971 t
ORIGIN      
        1 ggacgaaacc ggatacctga aagttgtacc gggtacaact aagaccaata ttccgggggt
       61 atttgctgca ggagattgtg cagataaagt ctatcgtcag gctgttaccg cagccggaac
      121 aggttgtatg gccgcgcttg atgccgaacg ctaccttgct gcgaaaggac attaaattca
      181 attacatgaa ataaaaaaag tcccggcaaa accgggactt ttttatttgt agtctataga
      241 ttgatcagtt attgataaaa agtttctctt ttgcaacacc ctgatcaaac acaacattaa
      301 ttgcgtagat accggatgca agttgagaaa cgtcgaatga gttgttactt ccattcaggt
      361 gaaaagtagc aaccacttta ccggagatat cagtaactgt taccactcca tttgtaagag
      421 aaggatcaag aacgtttatc ttaaggtttt tgttattgat gttcatatca accttatcac
      481 gaacattcaa ctcttcaacg ccaacggttt gctgacctgc aagaattgta gaacctgctg
      541 tttggtgtac agcataatct tttatagtga acgaattccc gttgatattt acatctaaac
      601 gtgcccaacc ataatgcaaa gagccgctga catccaaagc gagacccaga tatttattac
      661 ttgcgcccag ccagtcacca agaggaaaag ttccaaggcc tgttacattg gcaactgcac
      721 cgagcatcat tgctgatgaa gaaacagatg taggagcttg tgcaaaaccg agtgtattat
      781 tgattgcatc gttcgcgttc aattttgcag ctaaaggaaa gattccttga gaagaagatg
      841 ccgaagcaac cccatttgag ccaaacgtgt tgattacaac tccaacacct gtgtatggga
      901 atccacctaa agaaccggaa cgtgaagagc aggtaattac ataatcatca cctgttgcgt
      961 tatcaagatt taataaatag ctattaccag gtgttacagt ctggtcggta atatcagtgt
     1021 aaagaatctg agcattagca gaacctgcag caccgattgc tccggcaaca gcagcgtaac
     1081 gcatcatgcg gctttttttg ttagcgtcta attttttcat agcgaatgga atttggtcgt
     1141 gtaaatatag taaaatgcag tatcgaaaat tgttaaaatt tccccggttt gttatggatt
     1201 atcagacaac tcaaataatc ccgatacttg aatttcaaca gcttcatttt caatcagcca
     1261 atcggaatgc acgatttcca acaaaggcct tactgatgcc cattcaccaa atacccttct
     1321 ttgttcggta tttaacgtag ccaaatgtgt gggatagacc ttgttaacaa tctttgattc
     1381 ttgtcctgca cgttttaatt gtgccggaaa aagcagagaa tcatctgaat caagccaaat
     1441 caatttcagt ggacggtcct caggaatttt catcaactgt gcttgggtta acaggaaaat
     1501 tgcttcaggg ttttgattaa ttgattttat gttaaggtca gcctttaact ccggagaatc
     1561 aatagatgga cgtaaatcga tacactcaat gcctttggat ttaaggaatt cacaaagctc
     1621 ctcttgtccg gcagctggta aaccggaaac acacttaatt gtaaactttt ggaatttcgc
     1681 taaccagtca acctgctctt ttaatttttc aggatcaggt attcgggtgg tatataattc
     1741 cattaagcga tttcgtgcac gaagcattcc cggatgtatg tgcaatgcat taaggtaagc
     1801 agtttcggct ggtcgataat ccttgcataa tctgaaacat tctccaagct gaaaatgagc
     1861 tgcagcaaaa cgtggtttta attctagcgt atcaatgaaa aaatcgatag ctgtatctgc
     1921 ctcacctgac cgcaacaatg ccatgcctct tccaaaattt gcttcagcat tccgtggatc
     1981 atcatttgca atttctgaga acacattttc tgcctcccgg tattgtccca ttgaaagcag
     2041 aatattcccc ttttgcaaac gaatatcaat cgatgagggc gctatctcca acgctttttc
     2101 ataagaaaac aatgcttttt tcggacggta ttgcgcctgt gccattttcc ctctgaaaaa
     2161 ccagaccttt aaatcaagta ccgtatttat tccaaacaat gcatccgtat gcatttctgc
     2221 ttctttccat ttgcgaagac ggatataaca ctgaataatc aatgagcggt accggtgaaa
     2281 ttccggataa agtggaatat ttctctctaa tattttcgct gcctcttcgt ccctcttttg
     2341 aaaggaaaga ctttgtgcaa ggtaatattc aacttcagct tttgttttag ctactatttc
     2401 ttcattggtt ccttccggac gttcaatata acccaattca atcaattgct ctaatgcctc
     2461 acgcgaatcc catgtattca cccttaattc ttcaggatgc attccggatg aacaaccacc
     2521 atcagaatgg ttttcccatg aatctatcca atgaatttcc ttttgctccg aaaaacattg
     2581 ttgcaatact ttaccataca tatccattcc aacaggtaat cccatgtaat gaagaatggt
     2641 tggagtaata tctatcaccg atgaaccaaa aatttcggta tttgaatata taccaggtcc
     2701 tgaaaaagca atcattccat agggactgtg ttcagaagtt ggtcctgccg gttcaactgg
     2761 aatgacgcgt ggacgaagat tgccggattc aaaaccatga tcacttagaa tcagaatagc
     2821 tgtattatca tctattaaat ccagcattct gtccagcatc atatcctgaa accggtacgc
     2881 tccgtcgacc acctgattat aaaatatgaa tttttcatcc ggcactccat ccagtttcgg
     2941 tgctctgaat ttcatggcca tatgagagag atgatcaatc atatcgaagt aaacgcccat
     3001 tacatcccat tcggtatttt gcatcaacca tgtagatgca gcctgcaggc ttgctgcttt
     3061 tgcaatttca tttcgcagtg ttgtaattac tttgctctcc tcctcgggca tttgatatag
     3121 ttccggcaca aaaggtaaaa ccattgcagg agtaatttct gcagggtgaa cacgaaattg
     3181 cgaaagctgt tcagcgatat tttcagggtg tacagttccg ggcgccatca accaattttc
     3241 aggatcagga gaatttgaaa attggttgat gg
//
LOCUS       DAOMYG010000377         3247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10819_length_3247_cov_9.947368, whole genome shotgun sequence.
ACCESSION   DAOMYG010000377 DAOMYG010000000
VERSION     DAOMYG010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10819_length_3247_cov_9.947368"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1828)
                     /locus_tag="PK610_11245"
     CDS             complement(2..1828)
                     /locus_tag="PK610_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36558.1"
                     /translation="MNRFLRFIVINLVVFALCNKATAQFYNGSHMQFGKNRVQYNGFT
                     WMSQNYERFKVYYSMGGKIHAEYVAQSAHRQLQEIEKMFDFYISDKLEFIIYNSQSQF
                     RQSNVGITGDETYNIGGVTRMVGNKVFLFYEGDHRKLDRQIRAGLATVLINQMMYGAN
                     WRDVLKNSALLTLPEWYLQGFVSYVSEPWSVETEGFVKDGVMSGRYDRFYRLEGVHAQ
                     YAGHAMWNYIVEVYGQAVIPNILYMTRISRNVESGFVFVIGSGLRKLSADYVAYYKMK
                     FADDAKVLQTPTLEELPIKTKNNIVYQQFRVSPNGKHAAFVTNQMGQYKVWIQDLETH
                     SKKRLCKGEQKLNRINDYSYPVLGWHPTGQGLAYVVEKKGEVLFCLYSLEDGSTTRKT
                     LGKVDKVLSFDYSDDGKTLVFSAVSNGQTDLYIYRPTANALDQITDDIYDDLHPKFVH
                     GGTGIIFSSNRTSDTLRKDIKVKPYDAQKDIFVYNLRDKARVLKRITNTPNADESYPS
                     QLDTLNYTFLSDENGVVNRFAAIYDSVISYIDTVVHYRYFTSTAPLSNFNNNILEYDI
                     VEKKGIARTGMNTWPRAARRRPSSCCTTSTAPGPATVPSGAR"
     gene            1899..2564
                     /locus_tag="PK610_11250"
     CDS             1899..2564
                     /locus_tag="PK610_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ36559.1"
                     /translation="MSTVKVFTFNPFQENTYVVADPSGQCVIIDPGCFDPSEKRELAD
                     YISSNNLKPVKLLNTHAHIDHVLGNRFVAEKWGLGLELHREDLVTLHSLPNYAHIFGV
                     GTIEESPEPANFLKEGDKIQFGSTVFDVVFVPGHCPGHVAFINRAEEYVISGDVLFSG
                     SIGRTDLPGGSMKVLMESIQKKILPLGDNYKVYSGHGPLTTIGREKRENYFLQPGIAE
                     SLS"
     gene            complement(2534..>3247)
                     /locus_tag="PK610_11255"
     CDS             complement(2534..>3247)
                     /locus_tag="PK610_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRJ36560.1"
                     /translation="GDFTTIKLRRKVISGKTEDPYDTYSRWIPQLKNPEQLYRCFFLL
                     GNRAQFDRSIRFDHPQLVKLIKEIEQQIPVGIHPSYASHQDSTVFHKEVARLQSIIED
                     PVRYSRFHYLKFSLPESYRNLENAGILEDHSMGHASHAGFRAGTCFPFKFYDLEQERI
                     SSLEIIPLTAMDGTLCEYMGMSPEAAMQLSKKLWDEVSLFGGELVFLWHNETLNDKGK
                     WQGWYNVFLHQLRLSAIPG"
BASE COUNT          960 a          713 c          640 g          934 t
ORIGIN      
        1 ttcacctcgc cccagaagga accgtagccg gcccgggcgc cgtcgaggtc gtgcagcacg
       61 atgacggacg gcgtcgggcc gcccttggcc acgtattcat accagtcctg gcgatgccct
      121 tcttttcaac tatgtcgtat tccagaatat tattgttaaa gtttgaaagc ggggctgtgc
      181 tggtaaaata acggtaatgc accacagtat caatgtaaga gattacactg tcataaattg
      241 cagcaaatcg atttacaaca ccattttcat cacttaaaaa agtatagttg agtgtatcca
      301 actgtgaagg ataagactca tcagcgtttg gggtatttgt tattcgttta agtacccttg
      361 ccttatcgcg gagattgtaa acgaaaatat ctttttgggc atcgtaaggt tttactttta
      421 tgtcttttcg caaagtatca cttgttctat tggaggaaaa aataattccg gttccaccgt
      481 gaacgaactt aggatggaga tcatcgtaaa tatcatcagt aatctgatca agcgcatttg
      541 cagttggccg gtagatgtat aaatcggtct gcccatttga tacggcagag aacaccaaag
      601 ttttgccatc atcactataa tcaaaactta aaaccttatc tacttttcca agcgttttac
      661 gtgtggttga tccgtcttca agcgaatata ggcagaacaa aacttcacct ttcttttcaa
      721 ctacataagc caaaccttga ccggtaggat gccatccaag tactggatac gaataatcgt
      781 ttattctatt tagtttttgc tcgcctttac agagtcgctt tttcgaatgg gtttccagat
      841 cctgaatcca aaccttatac tgccccattt gatttgtaac aaaagcagca tgctttccat
      901 taggactaac cctgaattgc tggtagacaa tattattttt tgttttaatt ggcaattctt
      961 ctagtgtagg cgtttgaaga acttttgcgt catcggcaaa tttcatttta taataggcta
     1021 catagtcggc cgatagtttc cgtaatcctg aaccaataac gaagacaaac ccactttcaa
     1081 catttctcga aatacgagtc atgtatagaa tattggggat tacggcctga ccgtaaacct
     1141 caacaatata attccacatc gcatgtcctg catactgtgc atgcacacct tcaagacggt
     1201 aaaatctgtc gtaacgacca ctcattactc cgtcctttac aaatccctct gtttctacgc
     1261 tccatggctc agaaacatag gagacaaaac cttgcaggta ccactcaggc aatgttaaga
     1321 gcgcagaatt cttcaggaca tctctccaat ttgcgccata catcatttgg ttgatcagaa
     1381 cagtagctaa tcctgcacga atctgtcgat cgagtttacg gtgatcccct tcgtaaaaaa
     1441 gaaaaacctt gttaccaacc attcgtgtaa caccgccaat attgtacgtc tcatcgcctg
     1501 taattccaac attactctga cgaaactgag attgtgaatt gtaaataatg aattcaagtt
     1561 tatccgagat gtaaaaatca aacatcttct caatctcctg taactgacgg tgggcggatt
     1621 gagcgacata ttcagcgtga attttccctc ccatggaata ataaacctta aaccgctcat
     1681 aattctgcga catccaggta aatccattgt attgaactct atttttcccg aattgcatgt
     1741 gcgaaccgtt atagaactgc gcagttgcct tgttgcaaag ggcaaaaacg acaagattga
     1801 ttacaataaa gcgcagaaaa cgattcatag gcgaaaatgc attgtaaata taactaatca
     1861 gaaccaatct tagtattttt acagtctaag aagaacaaat gagtactgtc aaggttttta
     1921 cctttaaccc ctttcaggaa aacacatatg tggtagctga tccctcaggt caatgtgtta
     1981 tcatcgatcc gggctgtttc gatccctcag aaaagcgtga attggccgat tacatcagtt
     2041 caaacaacct gaaaccggtt aaacttctga atactcatgc ccacattgat catgttttag
     2101 ggaaccgttt tgttgctgaa aaatgggggt tgggactgga actccaccgt gaggatctgg
     2161 tcaccttgca ttcactaccc aattatgcac atattttcgg ggttggaaca attgaggaat
     2221 ctccggaacc tgctaacttt ttgaaagaag gagataaaat tcaattcggg tcaactgttt
     2281 tcgatgtagt gtttgttccg ggacactgtc cggggcacgt tgctttcatc aacagagcag
     2341 aagaatacgt gatttcaggt gatgtccttt tttccggcag tattggaaga accgatcttc
     2401 ctggcggatc aatgaaagtt ttaatggaaa gcattcaaaa aaagatactt ccattgggcg
     2461 ataactataa agtgtattcc ggacacggtc ctttaacaac cattggtcgt gaaaaaagag
     2521 aaaactattt tcttcaaccc ggaattgcag aaagcctgag ttgatgtaag aacacattat
     2581 accaaccctg ccatttaccc ttatcgttta aggtttcgtt atgccaaaga aaaaccaatt
     2641 cacctccgaa aaggctaacc tcatcccata gcttcttcga taattgcatt gcggcttccg
     2701 gcgacatgcc catatactca cagagggtgc catccatagc ggtcaatggt attatttcca
     2761 gcgaagaaat ccgctcctgc tccaaatcat aaaacttaaa cggaaaacag gtgcctgccc
     2821 gaaaacctgc gtgcgatgca tgtcccatac tgtgatcttc cagaatacct gcattttcaa
     2881 gattgcggta tgattcagga agagaaaatt tcaaataatg gaatcgtgaa tacctaaccg
     2941 gatcttcaat tatggattgc aatctggcga cctctttgtg gaatacagtc gaatcctgat
     3001 gcgaagcata agaaggatgg atgcccaccg gaatctgttg ttcaatttcc ttaatcaact
     3061 taaccagttg cggatgatcg aaacggatgg aacggtcaaa ttgcgcacgg ttgcctaata
     3121 agaaaaagca acggtacaat tgttcaggat ttttcaattg cggaatccag cgggaataag
     3181 tatcgtaagg atcttcagtc tttccgctta tcactttcct gcgtaattta attgtggtga
     3241 aatcacc
//
LOCUS       DAOMYG010000378         3243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10837_length_3243_cov_9.172522, whole genome shotgun sequence.
ACCESSION   DAOMYG010000378 DAOMYG010000000
VERSION     DAOMYG010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10837_length_3243_cov_9.172522"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="PK610_11260"
     CDS             complement(<1..1215)
                     /locus_tag="PK610_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36561.1"
                     /translation="MRTIYNTLFSGILLFAASALNAQNVEFKSSNFKDKKEEFKAATE
                     GIDKGDDFLKKGNALILETKDPGDNFLLAIYHYSKAQSLNPNNAELNFKLANAYLYTN
                     EKYKAKDHLMKAIQLDANVDKKQEFFLGLVYLYDAKWDDAMKAFKSFEGSKKADDYVK
                     FIAKHLEECKSGKTLMAKPERVWVDNISELNSESDDYSPSITMDGASMIFTSRRPNGH
                     SQQAWGGYDADVYTSDLVNGKWNTPKSIGAQINTDKDEVSSMLAYNGTKLLMFRNEAG
                     HFDIFESELRGSNWSAPINFARVINTVANQTFASYDFDDVKIYYLSDKEGGVSNSGTD
                     IYFSGVMDRAKRQYGNGMSVGSVINTRFNEGPIYMHPDGETMYFASQGHNSMGGYDIF
                     VSYKKQGQWLAPI"
     gene            complement(1221..2315)
                     /locus_tag="PK610_11265"
     CDS             complement(1221..2315)
                     /locus_tag="PK610_11265"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ36562.1"
                     /translation="MRKCLLTLAGLSITMLVQAQPDMMTEKERQYKDSIDALNAASAM
                     TAEFQTAYNDGNNLFAQRKFNDAISAYEKALGISNTFKEAWYNKGVAEIEAKKYTQAV
                     GSFTSLIKLNDQNGKAYFQRARAYQLNNDLKNAETDLMKAASLSPKNEKVFYNLGTVY
                     FLMKDYTKALEQFGKAITLKPDFAYAYNDRGSCYRMMEKYPEAVKEYDLAVKYNGKLP
                     FVLNNLGTAKRKLKDFSGALDAYNRALSIDPAFFLAYLNRGALNFDQGKWDEAIKDFN
                     KCIEIKGDYAPAYNNRGSAKMKKEDYRGALADFDKAIQLDPSYANAYVNRGTAKEMNR
                     DAAGACADWKKARELGAELGKKFFVNDCNE"
     gene            2623..3204
                     /locus_tag="PK610_11270"
     CDS             2623..3204
                     /locus_tag="PK610_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HRJ36563.1"
                     /translation="MLGLKLPTDPRWLALVESNISEILTDHAWCEQKAASNAISLIVR
                     HSEHPELVEEMGKIAVEEMEHFNLVVAKIKEKGLKLGAERRDDYVNDLMEFMIKGGNR
                     EQQLVDRLLFAAMIEARSCERFKMLSEKMKDADLREFYFSLMASEAGHYATFIGFARK
                     YSKSVNVDKRWQQFLDYEAGLMEKYGRKETMHG"
BASE COUNT          880 a          680 c          682 g         1001 t
ORIGIN      
        1 gatcggagca agccattgtc cttgcttttt gtacgatacg aaaatatcat acccacccat
       61 tgaattgtgt ccttgtgagg cgaaatacat ggtttcacca tcggggtgca tgtagattgg
      121 tccttcgtta aatcgggtat tgatgaccga accaacgctc ataccgttac cgtactgacg
      181 ttttgcacga tccattacac cgctgaaata gatatccgtt ccgctattgg aaacaccacc
      241 ttctttgtcg gagagataat agatttttac atcatcaaaa tcgtaagaag caaatgtctg
      301 gttggcgacg gtattgataa cacgtgcaaa atttatgggt gcactccagt tactacctcg
      361 cagttccgac tcgaaaatat cgaagtgtcc ggcttcgtta cggaacatca gcagtttggt
      421 accgttgtag gccagcatac tggaaacttc atccttatcg gtattgatct gtgcgccgat
      481 tgattttgga gtattccatt taccgtttac aagatcagaa gtataaacat cagcatcata
      541 accaccccat gcctgttgag aatgcccgtt tggacggcgt gaagtaaaga tcatacttgc
      601 accatccatt gtaatagaag gcgaataatc atccgattcg gaattcagtt cgctgatgtt
      661 atccacccaa acacgttcgg gttttgccat aagggttttt ccgcttttgc attcttcgag
      721 gtgtttggca atgaatttta cataatcatc tgcctttttg ctaccctcaa atgatttgaa
      781 cgctttcatg gcatcatccc atttcgcatc atagagataa accagaccga ggaaaaactc
      841 ctgtttttta tccacattcg catccagctg aatagccttc atgaggtgat ctttcgcctt
      901 gtatttctcg ttggtataga gataggcatt tgccagtttg aagttcaatt cggcgttgtt
      961 gggattgagt gattgtgctt tggagtagtg ataaatcgcc aaaaggaaat tatcgcccgg
     1021 atcttttgtt tcgagtatca atgcattccc ctttttcaaa aaatcatcgc ctttatcgat
     1081 tccctctgtt gcagctttaa attcttcctt cttatccttg aagttagaag atttaaattc
     1141 aacattctgt gcattcaatg ccgatgcagc aaagagcaat atgccggaga atagtgtgtt
     1201 gtatatcgtt ctcatagttc ttactcgttg caatcgttaa caaaaaattt cttacccagt
     1261 tcagctccga gctcacgtgc ttttttccaa tcggcacaag ctcccgcagc atcacggttc
     1321 atttctttgg ctgtaccacg gtttacataa gcgtttgcat aagaaggatc gagctgaatg
     1381 gctttgtcaa aatctgccaa tgcaccgcga taatcttctt tcttcatttt ggcactgcct
     1441 ctgttgttgt aggcaggagc ataatcgccc ttgatctcaa tgcacttatt aaaatcttta
     1501 atcgcttcat cccatttacc ctggtcaaaa ttcaatgctc ctctgttcag ataagccaga
     1561 aagaaagcgg gatcgatact gagcgcgcgg ttatatgcat ccaacgcgcc cgaaaaatct
     1621 ttcagtttac gttttgctgt accgagatta ttcagcacaa aaggaagctt tccattgtac
     1681 ttcacagcca ggtcgtattc cttaacggct tcaggatact tctccatcat ccggtaacag
     1741 gaaccacgat cgttataagc ataggcaaaa tcaggtttca gtgtaatggc tttaccaaac
     1801 tgttccaatg ctttggtata atccttcata aggaaatata ctgttccaag attgtaaaat
     1861 actttctcat tcttaggact aagcgatgcg gctttcatca aatcggtttc ggcgtttttc
     1921 aaatcgttgt tgagttgata cgcacgtgca cgctgaaaat aggccttacc attctgatca
     1981 ttaagtttga tcagactggt gaaactacct actgcctgag tatatttttt tgcttcaatt
     2041 tccgcaactc ctttattgta ccatgcctct ttgaaggtat tggaaattcc gagtgccttt
     2101 tcgtaggctg aaattgcatc gttaaacttt cgctgtgcaa agaggttatt accgtcgttg
     2161 taagcggttt ggaattctgc agtcatagcc gatgccgcat taagagcgtc gatcgaatct
     2221 ttgtactgcc gctccttctc cgtcatcatg tcgggctgcg cctgaaccag cattgttatg
     2281 ctcagtccgg ctaaagtcag taaacatttt ctcacgtgat gttgattttg gttggttttt
     2341 aaaaatacac ttcacaatcc caatgccaaa aggagttttt tcaacacatt gttaataact
     2401 gtggcttatt atcggttatc agttatcagt ataagagtgg ttggatagaa gttctgttca
     2461 ctgttctggt tagtagttat tagttataag ttatgggtaa cgagtgattg ggaagaagtt
     2521 ctgatcaccg ccactggcct gtcccgacca atcgcgccgg gatcacttca ccgttcaccg
     2581 ataactattg acacatatca ctaactttgt aaaaaaaaac aaatgctcgg actcaaactt
     2641 cccactgatc cccgctggtt ggcgctggtg gagagcaata tttcggaaat tctgaccgac
     2701 catgcgtggt gtgaacaaaa agcagcttcc aatgcaataa gtttaattgt caggcattct
     2761 gaacatcctg aattggtgga ggaaatgggt aagatagctg tagaggaaat ggaacatttc
     2821 aatttagtag ttgcaaaaat caaggaaaaa ggattaaaac tcggagcgga gcgcagagat
     2881 gattatgtaa atgatctgat ggaattcatg ataaaaggcg gaaatcgcga acaacaattg
     2941 gttgatcgtt tgctctttgc agcaatgatt gaagcaagaa gttgcgaacg gtttaaaatg
     3001 ctctccgaaa aaatgaagga tgccgatctc cgcgaattct atttttcact aatggcttcg
     3061 gaagccggtc attatgcaac atttatcggt tttgcgcgca aatacagcaa aagtgtaaac
     3121 gtggataaac gctggcaaca gtttttggat tacgaggctg gattaatgga gaaatatgga
     3181 aggaaagaaa cgatgcacgg gtgatttttt gttatcggtt atcggttatc agttattggt
     3241 tat
//
LOCUS       DAOMYG010000379         3243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10838_length_3243_cov_8.846299, whole genome shotgun sequence.
ACCESSION   DAOMYG010000379 DAOMYG010000000
VERSION     DAOMYG010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10838_length_3243_cov_8.846299"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..499)
                     /locus_tag="PK610_11275"
     CDS             complement(26..499)
                     /locus_tag="PK610_11275"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRJ36564.1"
                     /translation="MEHFTNGLSLAYTNPNRKGFGFFNREHQWVVRPTYDNATSFENG
                     FSIVEMNGQKGVIDITGKIIIPLKYQSIYANAITDGYFACEQGNKREYLNAKGLPVTT
                     IAVDYLLSAKGHLLMPYKSSENGKMGYLNRNGDIIIKADYEKAASFSEGKAWVII"
     gene            complement(931..2331)
                     /gene="gdhA"
                     /locus_tag="PK610_11280"
     CDS             complement(931..2331)
                     /gene="gdhA"
                     /locus_tag="PK610_11280"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HRJ36565.1"
                     /translation="MATKTVAKKASSKASSNGSGVHAKIKAFMSMVSAKNPNEPEFLQ
                     AVQEVAETVIPYIEANPKYNKAKILERIAEPERVIMFRVPWIDDKGEWQINRGFRIQM
                     NSAIGPYKGGIRFHPTVNLSVLKFLAFEQVFKNSLTTLPMGGGKGGSDFDPKGKSDNE
                     IMRFCQSFMTELERHIGADTDVPAGDIGVGGREVGYMFGQYKRLRNEFTGVLTGKGIT
                     FGGSLIRPEATGYGCVYFAAEMLATKGKDFKGKTVVISGSGNVAQYAAEKCLHLGAKV
                     ITMSDSSGYIVDKDGIDAKKLAYIMELKNEKRARISEYTKKYKSGKFVAGKTPWAEKC
                     DIALPCATQNELHGKDAETLVKNGCYCVAEGANMPSTIDAINHFVAKKILFAPGKASN
                     AGGVATSGLEMSQNSLRLSWTKEEVDQRLHNIMKGIHATCVKYGKDGDFVNYVKGANI
                     GGFVKVAEAMIGQGHV"
     gene            2544..2849
                     /locus_tag="PK610_11285"
     CDS             2544..2849
                     /locus_tag="PK610_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36566.1"
                     /translation="MKIILAGIFLLALPFEGMSKDKLEIINASYIEKMMKWIPGGTVK
                     MAPIQETIGEKGTEFTVENFYMQEAEVSNFQYVSFLYDLVKKADTNSLRKAIPDTTV"
     gene            2868..>3243
                     /locus_tag="PK610_11290"
     CDS             2868..>3243
                     /locus_tag="PK610_11290"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRJ36567.1"
                     /translation="MNSFVEHYLRHPAYRNYPVVGISYEQTKMFCIWLTERYNADPKR
                     KHKEVLFELPTETEWMNAASGGMNSAIFPWGGHYLRNAKGEYLANFSQINEASIIPDS
                     CGGQKVFRWTALFRLGMLRNKAT"
BASE COUNT          891 a          708 c          679 g          965 t
ORIGIN      
        1 aacatccttc ttgattaaac cctgattaaa ttattaccca tgcttttcct tcactaaagg
       61 aggctgcttt ttcgtaatct gctttaatta ttatatctcc attccggttc aggtagccca
      121 tttttccgtt ttcgctcgac ttgtacggca tcagcaaatg acctttagcc gataataaat
      181 aatccacagc aattgttgtt acgggcagtc cttttgcgtt gagatattct cgtttattcc
      241 cttgctcaca ggcaaaataa ccatctgtaa ttgcgttggc atatatgctt tggtatttga
      301 gtggaatgat gatctttccg gtgatgtcaa tcactccttt ttgaccattc atttcaacaa
      361 tggaaaaacc attttcaaaa ctggttgcgt tatcgtaggt cggcctcacc acccattgat
      421 gctcccggtt gaaaaatcca aaacccttac ggttgggatt ggtataggca agcgataatc
      481 cgttggtgaa atgctccatg ttggtggcga gtttcggtgt aaaaaccaca tcccctcttt
      541 cttgtaagat tttttcgaaa tgatttttcc gtttgtgttt atcaattgaa aatcgccacc
      601 cggcactttg atccacgcta ctccgtcacg aaaatcagat gctttttcaa attgggacgg
      661 aataacaagt ttgcctgttg tatcaatata gccgtatcgc cattgattgt tactcagcat
      721 ttgcacccgt gccattcctc cggaaaaatc agccgctctc ctttttcatt cagacagccg
      781 aattttttct gggctcctgc tgataaagtc aggattgaaa acacaagtaa agagaaccga
      841 atcatgacaa cttgttttgt tattcaagat aataaaaaaa gccctcgcat cgcgggggct
      901 tttttttatt gcgtgatgtt gtaaactgaa ttaaacgtgg ccttgaccaa tcatcgcttc
      961 agcaacttta acgaagcctc cgatgtttgc acctttcacg tagttcacaa agtcaccatc
     1021 cttaccgtac ttcacgcagg tagcatggat gcctttcatg atgttgtgca gacgctgatc
     1081 aacttcttcc ttggtccagg aaagacgaag tgagttctgg ctcatttcaa gacctgaagt
     1141 agctacgcct cctgcattgg aagctttacc gggagcgaag agaatcttct tggcaacaaa
     1201 gtgattaatg gcatcgatgg tagaaggcat gttagctcct tcagcaacgc agtaacaacc
     1261 gtttttcaca agtgtttcag catctttacc gtgcaattca ttttgtgttg cgcagggaag
     1321 tgcaatatcg catttctcag cccatggagt ttttccggca acgaatttac cgctcttgta
     1381 ttttttagtg tactcgctga tacgcgcacg cttttcattt ttaagttcca tgatgtaggc
     1441 aagtttcttc gcatcgatac cgtctttgtc aacgatataa ccagaagaat cactcatggt
     1501 gattactttc gcgccaaggt gcagacactt ttcagctgca tactgagcaa cgtttcctga
     1561 acctgaaata acaaccgtct tacctttgaa atccttgcct tttgtagcga gcatttctgc
     1621 agcaaaatat acacaaccgt atcctgtagc ttccggacga atcaacgacc caccgaaagt
     1681 gataccctta ccggtaagta ctccggtaaa ctcgttacgc agacgcttat actgaccaaa
     1741 catgtaacct acctcgcggc cacctactcc gatgtcacct gcaggaacgt cggtatcggc
     1801 acctatgtga cgctcaagct cagtcatgaa gctttggcag aaacgcatga tttcgttatc
     1861 tgatttgccc ttaggatcga agtcggaacc acctttacca ccacccattg ggagtgtagt
     1921 aagtgaattc ttgaacacct gctcaaacgc aaggaacttc aatacgctaa ggttaactgt
     1981 aggatggaaa cggataccgc ctttgtaggg accgattgca gagttcatct gaatacggaa
     2041 accacggttg atctgccatt ctcccttatc gtcgatccag ggaacacgga acatgatcac
     2101 acgctcaggc tcggcaatac gctcgaggat cttcgctttg ttgtacttgg gattcgcctc
     2161 gatgtaagga atcaccgttt ctgcaacctc ttgtacggcc tgaagaaact caggttcgtt
     2221 ggggtttttc gcgctcacca tgctcatgaa agccttgatc tttgcatgaa caccggagcc
     2281 attggaagaa gccttggaag aggccttttt tgcaacagtt ttggtagcca taatttttct
     2341 agttagcggg tcaaatgtaa atgttttttt tactcgccca atgccatttt tctggctgag
     2401 attctcaagt taatgtgctg gttattagca gctattttag cggccgttca attaccttac
     2461 ttcttttgag aagaataatt cggttacagg atcaggtttg taaccgaatg aggcaatttg
     2521 cttatagtgt aaacaaatcg aacatgaaga ttattctagc tggaattttt ttattggcgc
     2581 ttccttttga aggaatgtcg aaggataaac tggaaattat caatgccagt tacatcgaaa
     2641 agatgatgaa atggataccg ggagggacag tgaaaatggc tcctatacaa gagacaatag
     2701 gtgaaaaagg aactgagttc acagttgaga acttttacat gcaagaagca gaggttagca
     2761 atttccaata tgttagtttt ttatacgatt tggtaaaaaa ggcagacact aattctttac
     2821 gaaaagcaat tcccgacaca actgtatgaa tcgaaaaaca tgcctatatg aattcttttg
     2881 ttgagcatta tttgcggcat cctgcatatc ggaactatcc ggttgttggc ataagctatg
     2941 aacaaacaaa aatgttttgc atttggctca ctgagcggta taatgcagat cccaaacgga
     3001 aacataagga ggtattattc gaattaccaa ccgagacaga gtggatgaat gcggcatcag
     3061 gtggaatgaa tagtgcaatt tttccatggg gaggacatta ccttcgaaat gcaaaaggag
     3121 agtatctggc caatttctcg caaataaatg aagcatcaat tattccggat agttgcggag
     3181 gacaaaaagt atttcgatgg acagcgcttt ttcggttagg tatgctgaga aataaagcaa
     3241 ccc
//
LOCUS       DAOMYG010000380         3239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10853_length_3239_cov_9.510678, whole genome shotgun sequence.
ACCESSION   DAOMYG010000380 DAOMYG010000000
VERSION     DAOMYG010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10853_length_3239_cov_9.510678"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..1269
                     /gene="ychF"
                     /locus_tag="PK610_11295"
     CDS             175..1269
                     /gene="ychF"
                     /locus_tag="PK610_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRJ36568.1"
                     /translation="MKCGIVGLPNVGKSTLFNCISSGKAQAANFPFCTIEPNIGMTNV
                     PDERLYKLETLVKPERVLPATVEIVDIAGLVKGASKGEGLGNQFLANIRETDAILHVL
                     RCFEDGNIVHVDGSVNPVRDKEVIDTELQLKDLESVEKGMARVEKQAKTGSDKDAKKK
                     YDLIVAIKAHLEQGKNVRSMELSVEDKAAIADLHLLTVKPVLYVCNVDEASVKSGNAH
                     VDAVKNAVKAEGAQVCIITAAMESEIAALETFEERKMFLDELGLEEPGVNKLIRAAYS
                     LLELQTYFTAGVKEVRAWTIYKGMTAPQAAGVIHTDFEKGFIRAEVIHYEDYIKYGSE
                     TACKDVGKLSVEGKEYIVQDGDVMHFRFNV"
     gene            1282..1950
                     /locus_tag="PK610_11300"
     CDS             1282..1950
                     /locus_tag="PK610_11300"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRJ36569.1"
                     /translation="MINLHSHHFDPSAEVVVMQADSDSLVQLAERFPTAYFSIGIHPW
                     NVGSDYEVCLQKLEVFLNKPDFKSRIIALGECGLDKLRNQENFSKQEACFSKQIMLAE
                     KHGLPLLIHCVKAHQECSTLLLKEKVSVPIVFHGWNNRETILAPLLKNGWNVSFGSAL
                     LNENSQAGQQIVTIPRDRIFLETDDSELPIEKIYSAAEHLMGISHSELQKIIARNFSK
                     FCRL"
     gene            1947..2684
                     /locus_tag="PK610_11305"
     CDS             1947..2684
                     /locus_tag="PK610_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HRJ36570.1"
                     /translation="MKETAWLGRTKLLIGDERLTKLMSSNVLIVGLGGVGSFAAEFIV
                     RAGVGKVTICDGDIVDPTNRNRQLPALSTTHGVSKAAWMQERLLAINPELQLTVINEF
                     LRMQPMKELLFSQRYDYVIDAIDSITPKITLITTCHQNNIPIISSMGAGGKLDPTRIR
                     VTDISNTFNCKLAHYVRKRLHRQKIYKGVKAVFSSEKAPAGSLMLTDGTNFKKSAYGT
                     MSYLPAAFGGACASVVIRDLIQWKETV"
     gene            complement(2688..>3239)
                     /locus_tag="PK610_11310"
     CDS             complement(2688..>3239)
                     /locus_tag="PK610_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36571.1"
                     /translation="AWERSIPGSTALNRDTSYNYHKWFHLKAEFQNYYKSKGWFRLGV
                     HFEGVYSIQPFLNNYTATILSAPYYHPIPESKTLFISNLRAFQYVAAGHQIIFHVKKN
                     IDWRLEAYAFQPFSRIESGPDNQAQYGELLKKRYLIASTAIVYHSPFGPASVSFNYYP
                     DQDRPFSLLFNMGYIIFPRHAIK"
BASE COUNT          963 a          586 c          756 g          934 t
ORIGIN      
        1 ttaccagaac aattgggatg gtaaacattt caaaaatggc aagccggtta gcgacggaac
       61 ctatttctac atcctttata ttacaaggaa agacaaggtt tactcaggaa ctgttacagt
      121 tctcggcgga aaatgataat ttcgcgcctc attcattgag gtaatattat gggattgaaa
      181 tgtggaattg tgggcttacc aaatgtcggt aagtccacat tgtttaattg catatcgagt
      241 ggaaaagcgc aagcggctaa tttccctttt tgcacaattg agccgaatat tgggatgacc
      301 aatgtgccgg atgaacgttt gtacaaactg gaaacacttg ttaaacctga gcgtgtactt
      361 ccggcaacgg ttgaaatcgt agatattgca ggtcttgtta aaggagcaag caaaggcgaa
      421 ggattgggga accagtttct cgccaatatc cgtgagacgg atgccatttt gcacgtattg
      481 cgatgttttg aagatggcaa tattgttcat gttgatggat cggtgaatcc tgttcgcgat
      541 aaagaggtca tcgataccga attgcagctg aaagatctgg agtcggtcga aaaaggaatg
      601 gctcgtgtag aaaagcaggc caaaaccgga agcgataaag atgccaagaa gaaatacgat
      661 ctgatagtgg ccattaaagc acatcttgag cagggaaaga acgtacgcag tatggaactt
      721 tcggttgaag ataaagctgc aattgctgat ttacatttac taacggttaa acctgttttg
      781 tatgtatgca atgtggacga agcctcggtt aaatcaggaa atgcgcatgt tgatgccgta
      841 aaaaatgcgg ttaaagcaga aggcgctcag gtttgcatta tcactgctgc aatggagtcg
      901 gaaattgctg cattggaaac ctttgaagaa cgcaagatgt tccttgatga gttaggactc
      961 gaagaacccg gagtaaataa gttgattcgc gctgcctatt ccctgcttga attgcaaacg
     1021 tattttactg caggagtgaa ggaagtacgt gcatggacta tttacaaagg aatgactgca
     1081 ccacaggctg ccggagtaat tcacaccgat tttgaaaaag gattcattcg cgcagaagtg
     1141 attcattacg aagattacat caaatacggt tctgaaacag cgtgtaaaga cgttggaaaa
     1201 ctttcggttg aaggaaaaga atacattgtg caggatggtg atgtgatgca cttccgtttc
     1261 aacgtgtaag ttacttttcc tatgatcaat ttgcattctc atcatttcga tccttcggct
     1321 gaggtggtgg ttatgcaggc agatagtgat tccctggttc aacttgctga acgatttcct
     1381 acggcttatt tttcaattgg aattcatccc tggaatgttg gaagtgatta tgaagtttgc
     1441 ctgcaaaaac tggaagtttt cttaaataaa cctgatttta aatcgcgaat tattgctttg
     1501 ggcgaatgcg gattggataa actcagaaat caggaaaact tttccaaaca ggaagcttgc
     1561 ttttctaaac aaatcatgct ggcagaaaag catggattac cgttgttgat tcactgcgtt
     1621 aaagcgcatc aggaatgcag cacgctttta ttaaaagaga aagtctctgt tcctatagta
     1681 tttcacggtt ggaataaccg ggaaacaata ttggctcctt tactgaaaaa cggttggaat
     1741 gtttcatttg ggtctgcttt gctcaatgaa aattcgcaag ccggtcaaca aattgttaca
     1801 atacctcgtg accggatttt tctggagacc gatgacagtg aattgcctat cgaaaaaata
     1861 tattctgctg cggaacatct gatggggatt tctcattcag aacttcaaaa aattattgca
     1921 agaaattttt caaaattctg tcgcttatga aagagactgc atggttggga agaacaaaac
     1981 ttcttattgg tgatgaacgt ctgacgaaat tgatgagctc caatgtattg attgttggtc
     2041 ttggaggtgt tggttccttt gctgcagagt ttattgttcg tgcaggtgta ggaaaggtaa
     2101 cgatttgcga tggggacatc gttgatccga caaaccggaa tcgccaatta cctgcacttt
     2161 ccacaacgca tggtgtctcc aaggcagcat ggatgcagga acgtttactg gcgatcaatc
     2221 cggaactgca actcacggtt atcaatgagt ttttgcgaat gcagcctatg aaggaattgc
     2281 ttttttcgca gcgatacgat tatgtgattg atgcaatcga ctccattact ccaaaaatta
     2341 cattgatcac tacatgtcac cagaacaata ttcctattat ctcttcaatg ggtgcaggag
     2401 gaaagctgga tccaacgcga atccgtgtta cagatatttc gaataccttc aattgcaaac
     2461 tcgcccatta tgtgcgcaaa cgattacacc gacagaaaat ttacaaaggt gttaaagcag
     2521 tattttcttc cgaaaaagca cccgcaggtt cattgatgct taccgatgga acgaatttta
     2581 aaaaatcggc atacggaacg atgtcgtact tgcccgctgc atttggaggt gcatgtgcaa
     2641 gtgttgtgat tcgcgatttg attcaatgga aagaaacagt ctgatgccta tttaatggca
     2701 tgacgtggga aaatgatata acccatattg aacaataacg aaaacggacg atcctgatcg
     2761 ggataataat tgaaacttac cgatgccggt ccgaatggac tatgatatac gattgctgtt
     2821 gaagcaatga ggtatctttt tttcagcagt tccccgtatt gtgcctgatt atcgggtccc
     2881 gattctattc tgctgaatgg ctgaaatgcg tacgcttcta atctccagtc aatatttttc
     2941 tttacatgga aaatgatctg atgtcctgct gcaacatatt gaaaagctct aagattcgaa
     3001 atgaagagtg ttttgctttc tggaatagga tgataataag gtgcggataa tattgttgca
     3061 gtgtagttat ttaaaaaggg ttgaatggaa tacacgcctt caaaatgaac acccagtcta
     3121 aaccatcctt ttgatttgta ataattctgg aattcagcct tgagatgaaa ccatttgtgg
     3181 taattgtagg aagtgtcgcg atttaatgcg gtagacccgg gaatggatct ttcccaggc
//
LOCUS       DAOMYG010000381         3218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_10957_length_3218_cov_11.435030, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000381 DAOMYG010000000
VERSION     DAOMYG010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10957_length_3218_cov_11.435030"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2272
                     /locus_tag="PK610_11315"
     CDS             <1..2272
                     /locus_tag="PK610_11315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ36572.1"
                     /translation="EGGATTGIWQVLNGSGTLATPTNLTTTYQPSVSDFAQGNVIFVL
                     SSTGNCNPVHDTVRVNFIQSPTVDAGLGNTFCKNNISPISISGSFLFAAGASWSGGNG
                     GAFGNSGAASTTYTPSPADLAANSLTLYYTTAGSFFACPNAIDSVIINFTNPPVVSPG
                     PNQTVCASTSAIPLTGSITGPTTTGIWSTTSSGAFSPSATNLTGNYLVNPSDTAAGSL
                     YLVLTSTNNGSCNAVRDSILITFVDIPQVQITSQDTVCANTNSVNLSGIISLGFTPTW
                     STNGAGSIAAPSNLNTVYTVNTADTAAGFIQVILSTNAGICPSQRDTMILVFSAPPVA
                     NAGPNQAYCNNELVLLTGIVTGATSTGVWSSTGTGLFNPGVNFLTTTYAPSALDVANG
                     SVNLILTTTNNAGCNPDTDVMTITFKDAPVAEFTNSNDCVGQNLQFTDASVATPNTIT
                     SWNWDFGDFTNSIASSPLHPYSASGTYIVTLIAGSSNGCFDTIQHPVTVYPLPIANFT
                     ATNPCEGTAVQFTDQSFISSGAVTQWQYNFGDGSPNATTQIASHVFPGTLTYVVTLQV
                     TSAFGCVDTVMIPVNILPRPDAAYTATPNPVLAFEPVQFTDQTTSGTPIIDWFYNFGD
                     STGINLQNPVHAYANGGAYTTYLIATDQNGCRDTAYLEITVSLMPAVPTGFTPNGDGE
                     NDVFRVRGGPFEALLFRVYNNWGQLIYESTDQSEGWNGEFKGTDQPMGVYTWTVEVVI
                     IGGKKIYLTGDVTLMR"
     gene            2277..>3218
                     /locus_tag="PK610_11320"
     CDS             2277..>3218
                     /locus_tag="PK610_11320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRJ36573.1"
                     /translation="MKKGILIIVFALSILSGRSQDFHLSMYDAAPLFLNPAMTGVFEG
                     DWRLHAQQRTQWRSVNFKPYNTSLLSFDAGAKKWGFGAQIMNMRAGIGNYNALQALGS
                     VAYTLPIDRNKNHNISFGLQGGITQKSVEYQLLTYDNQYVTSNGGGFDNTLNSGENFA
                     GNRLLLPQLNAGILYYYSKQQSRLNPFVGVSAFNLLEPNESFFNNNNKLPMRFYGHTG
                     VRVNFTEFFYIIPKVLFMSQTTAKELTFASDFGFFAKSSEVLFLAGIIYRNKDAGILS
                     VGARKGNFTGKISYDINTSSLTTASNGKGGFEISITYI"
BASE COUNT          945 a          739 c          638 g          896 t
ORIGIN      
        1 agaaggcgga gcaacaacag gaatctggca ggttttaaat ggatcaggaa ctttggctac
       61 accaaccaac ctaaccacaa cctaccaacc ctctgtaagt gattttgcgc aaggcaacgt
      121 aatttttgtt ttatcatcca ccggaaattg taatccggtt catgataccg ttcgcgtaaa
      181 cttcattcaa tcgccaactg ttgatgctgg tttaggaaat accttctgta aaaacaacat
      241 ctcccccatt tcaatcagcg gatcatttct attcgccgca ggtgcaagtt ggtctggtgg
      301 aaatggtggc gcatttggaa attcaggagc agcttcaaca acctatacac cttcacctgc
      361 tgatcttgcg gcaaattcac tgacactcta ttacaccact gcaggaagtt tctttgcttg
      421 tccgaatgcg atcgattctg tgatcatcaa tttcaccaat ccgcctgttg ttagtcccgg
      481 tccgaaccaa acagtttgcg cttcgacctc cgcaatacca ttaacgggat caattacagg
      541 tcctactact accggtattt ggtcaacaac atcatccggt gcattttcac cgtctgcaac
      601 caatcttaca ggtaattacc tggtaaatcc atctgatact gcagccggtt cattgtatct
      661 ggttctaact tctaccaaca atggaagctg taatgcggtt agagatagta ttctgatcac
      721 atttgttgac attcctcaag tgcagatcac atctcaggac acagtatgtg caaatacaaa
      781 ttcagtaaat ttaagtggaa ttatctccct tggatttact ccgacatggt cgaccaatgg
      841 tgcaggttct attgcagctc catctaacct gaataccgtt tatactgtta acacagcgga
      901 tactgctgca ggattcattc aagttatcct gagtacaaac gcaggaattt gcccatcaca
      961 gcgcgacacc atgatacttg tattttcggc acctccggtt gccaacgcag gtccgaatca
     1021 ggcgtattgt aacaatgaat tggtattact cacaggaatt gtaacaggag ctacctccac
     1081 aggtgtttgg tcttctacag gaacagggtt atttaatccg ggtgtgaatt tcctaacgac
     1141 cacttacgca ccatcagcac ttgatgttgc caatgggagt gttaacctga tcctcacaac
     1201 gaccaataat gcaggatgta atcccgatac cgatgtaatg accattacat ttaaagatgc
     1261 acctgttgct gaattcacaa actccaatga ttgtgtgggg cagaatttac aattcaccga
     1321 tgcatctgta gccacaccaa atacaatcac aagttggaat tgggatttcg gcgatttcac
     1381 caattcgatt gcatcttctc cactccaccc ctactctgca agcggaactt acatagttac
     1441 actaattgca ggatcaagca acggatgttt cgataccatt cagcatcctg ttacagttta
     1501 tccacttcct attgcgaatt tcactgcgac caatccttgt gaaggaacag cggttcagtt
     1561 taccgaccaa tcctttattt catcaggtgc ggttacacaa tggcaatata attttggcga
     1621 cggatcgcca aatgcaacta cacaaattgc atcgcatgtt ttccctggta ctctaactta
     1681 tgtagttaca ttacaggtaa cttctgcatt cggttgtgtg gatacggtaa tgattcccgt
     1741 aaacattctt ccgcgtccgg atgctgcata caccgcaacg ccaaatcctg ttcttgcatt
     1801 tgagccggta caatttacgg atcaaaccac ctcaggcact ccaattattg actggtttta
     1861 caatttcggt gattcaacag ggatcaatct tcaaaaccct gttcacgctt atgcaaatgg
     1921 tggagcatat acaacctacc tgattgcaac tgaccaaaac ggatgccgag atacagctta
     1981 ccttgaaata acagtatcgc tcatgccggc agtaccaaca ggtttcacac caaatggtga
     2041 tggagaaaac gatgtattcc gcgtaagagg tggtccgttt gaagcattgt tattcagagt
     2101 atacaacaac tggggacaac tcatctacga aagcacagat cagtcggaag gctggaatgg
     2161 tgaatttaaa ggaacggatc agcctatggg agtttacacc tggaccgttg aagttgtgat
     2221 tattggagga aagaaaattt acttaacagg tgacgtcacc ttaatgcggt aagcatatga
     2281 aaaaaggtat attaataatt gtatttgctt taagcattct atcaggaagg tcgcaggatt
     2341 tccacctctc aatgtacgat gcagcaccct tatttttaaa cccggcaatg accggtgttt
     2401 ttgaaggcga ttggagattg catgcacaac aacgtaccca gtggcgttct gtaaatttca
     2461 aaccttacaa cacctcacta ttaagcttcg atgcgggagc aaagaaatgg ggatttggcg
     2521 ctcagatcat gaacatgcga gcaggaattg gaaattacaa tgcactgcag gcattgggat
     2581 ctgttgctta tacattgcct atcgaccgaa acaaaaatca caacatttcc ttcgggttac
     2641 aaggaggtat cacacaaaaa tcagtggaat accaattgct tacctacgac aaccaatatg
     2701 taacgagtaa cggcggagga tttgacaata ccctcaacag cggagagaat tttgcaggaa
     2761 accgattgct tcttccacaa cttaacgcag gcatacttta ctattacagc aaacagcaaa
     2821 gcagacttaa tccctttgtt ggtgtttctg cattcaatct tctggaaccc aatgaaagtt
     2881 tctttaacaa caacaataaa ctgccaatgc gtttttacgg ccataccgga gtgcgcgtaa
     2941 actttactga attcttttac atcattccta aagttttgtt catgtcgcaa accaccgcca
     3001 aggaattaac atttgcaagt gactttggct tttttgccaa atcaagtgaa gtgctatttc
     3061 tggcaggaat catttaccgt aacaaagatg caggaatact ttcggttgga gcgcgtaaag
     3121 gaaatttcac tggtaaaatc agttatgata taaatacttc atcacttaca actgcaagta
     3181 atggaaaagg tggctttgaa atttcaataa cctatatc
//
LOCUS       DAOMYG010000382         3210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_11005_length_3210_cov_7.883043, whole genome shotgun sequence.
ACCESSION   DAOMYG010000382 DAOMYG010000000
VERSION     DAOMYG010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11005_length_3210_cov_7.883043"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(416..658)
                     /locus_tag="PK610_11325"
     CDS             complement(416..658)
                     /locus_tag="PK610_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36574.1"
                     /translation="MKKNLIILATFIGALSLSSCEKEYMGPLDAAGNPVLVRGGEGET
                     NTSSGGGENGVTDTGHDSDYDNTGVRRNKKPTPTTN"
     gene            complement(720..2531)
                     /locus_tag="PK610_11330"
     CDS             complement(720..2531)
                     /locus_tag="PK610_11330"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRJ36575.1"
                     /translation="MSNKVTSLYADRSGVLWIGNRDGLTRFSNNEFRSFSDNEGLVGG
                     NIRGIFRDESGTFWLGSALGMYRMKGGEFKLFQHEDPSLDNTVYCITSAGKNSLWVGS
                     KIGLYLFSGGIFSKVDLGESVNANNINFLIRDEDRLWVGTNNGIYELMADEFMRTGKV
                     EYRNYSRLEGVRGLESNMNAAYKDSKGSFWFGTDGGLVNYDPGKRKSTGNLFEPYIHI
                     DDVRMYFDQVDWKKYANGIDPETGLGKDLVVPYNKNHFTFYFTGISHTNPLKVRYRFM
                     LAGFDEEWSPVTDLRSITYSNLPYGKYTFRVIAGNGSDVWSSAPAEFSFVITPPFWLT
                     WWFFVLVAVVVIGLVIGVYRWRISVIKQKTEREQLIYKSKLLSLEQQTLNASMNRHFI
                     FNALNSIQYYINKQDKLEANRYLTSFAKLIRKNLDSSSTGNLVTLNEELERLELYLSL
                     ENMRFKNKFSYELYIDEDIDTEGVMIPPMLLQPYVENAIWHGILPNEQHGTIWIRIEP
                     FEERSIRITITDDGIGITTSLKNKQNNGSDHVSRGIQINSGRLAVLKKLTNENLRIVG
                     PYETSNEQGMVTGTCVELIIPGAGQLQADNFEESLQN"
     gene            complement(2634..>3210)
                     /locus_tag="PK610_11335"
     CDS             complement(2634..>3210)
                     /locus_tag="PK610_11335"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ36576.1"
                     /translation="RRHLRPRAVIGHSMGGMLSLKLAQAQPDLMDQLVLLAPVVRGRI
                     GKLVELDKLVTSDVGDYAIQRSEALWKLVQSDMVDVIAPLLGRPGMADEHSILRIRQD
                     VKRSHWRAAVHGIRSIARENLGPHLAEIKQPTLVIVGSRDQTVPPEEGQYAAEHLPQA
                     ELLELAGLSHQVLDESPRRVVDAVRQFLKPV"
BASE COUNT          860 a          809 c          647 g          894 t
ORIGIN      
        1 atcgtataca tccattttat cgatagcatc aaacaggatc tcataattat tggcttcacc
       61 caaagtatgc ctggaactaa aaatcttgaa ataccgcttt tccggcttgg tcaaagcctt
      121 tatcaaacga tgtagctggt ctgaagcgat gttagacatg tatatttatt taagattttt
      181 gttttgaagc aggcctttga agcgtaatta ttccactctt cgggccctat tcaaatagct
      241 ggtctctgcc tttatttaca gggatataaa gaacaaagca ttcttattaa atatacgaaa
      301 aaccatgtat tagaactgaa agaaataagc actattccgt tacctggaac cgccatagac
      361 atgtataaaa aaagccccgg aaatccgagg ctttactatc gatagaaaat cacaatcaat
      421 ttgttgttgg cgtaggcttc ttattacgac gcacacccgt attatcataa tctgaatcat
      481 ggccggtatc tgtaacaccg ttttcacccc caccggagct ggtattcgtt tcgccttcgc
      541 caccacgaac aagcacagga ttccctgcag catcaagtgg ccccatgtac tctttctcgc
      601 aagaactcaa tgagagcgct ccgataaaag ttgccagtat gattaagttc tttttcataa
      661 agtcagttac attacttctg cacttactgc ttctacgtac aaaattaaat aaaagttcct
      721 caattctgca aactttcctc aaaattatct gcctgtaact gtcctgcccc aggaataatg
      781 agctcaacgc aagtgcccgt aaccatacct tgctcattac tagtctcata aggaccaaca
      841 atccgcaggt tttcattggt aagctttttt agaaccgcca atcgcccact attgatctga
      901 attccccttg aaacatggtc ggagccgttg ttttgcttgt ttttcagact agtagtgatc
      961 ccaattccat cgtcggtaat agtgattcgg atggagcgct cctcaaatgg ctcaatcctg
     1021 atccagatgg ttccgtgctg ctcattggga aggattccat gccagattgc attttcaacg
     1081 tagggttgca atagcattgg gggaatcatt actccttctg tatcgatatc ttcatcgata
     1141 tacaattcat aagagaattt attcttaaaa cgcatgttct ccaatgaaag ataaagttcc
     1201 aatcgttcca gctcttcatt tagggtaaca agatttcctg tagatgaact atcgagattt
     1261 ttgcggatta gttttgcaaa actggtgagg taacgattcg cttccagttt gtcttgctta
     1321 ttgatgtagt actgaatgga gttgagcgca ttaaaaataa aatgcctgtt catgctcgca
     1381 ttcaatgttt gctgctcaag actcaatagt ttggatttat aaatgagttg ttcgcgttct
     1441 gttttctgtt ttatcacaga aatcctccaa cggtaaacac cgataacaag gccaattaca
     1501 accactgcaa ccaaaacgaa aaaccaccag gttaaccaga acggcggtgt aattacaaat
     1561 gaaaactctg caggagccga cgaccataca tcgctaccat tacctgcaat tacccgaaat
     1621 gtatattttc cgtatggaag attggaatag gtaatagagc gcaaatccgt tacaggcgac
     1681 cattcttcat caaatccggc aagcataaac cggtacctaa ccttgagtgg attggtgtga
     1741 ctgattccgg taaaatagaa ggtgaaatga tttttgttgt aaggcacaac cagatcttta
     1801 ccgagtcctg tttccggatc aattccgttt gcatattttt tccaatccac ttgatcaaaa
     1861 tacatgcgga catcatcaat gtggatataa ggttcaaaca gattccctgt gctttttcgc
     1921 ttgccgggat cgtaattgac caatccgcca tctgtaccaa accaaaatga gcctttcgaa
     1981 tctttgtatg ccgcattcat attgctctcc agtccgcgca caccctcaag ccgggagtag
     2041 tttctgtatt ctacctttcc ggtgcgcatg aattcgtcgg ccatcaattc gtaaatgcca
     2101 ttattggtgc ccacccatag acgatcctca tcgcgaatta aaaaatttat attgtttgcg
     2161 ttaacacttt ctccaagatc taccttgctg aatatccctc ccgaaaacaa atacaaacca
     2221 atcttgctgc ctacccataa actatttttc cctgcactgg tgatgcaata aacggtgtta
     2281 tcaagagaag gatcttcgtg ttgaaaaagt ttaaattctc ctcctttcat ccggtacatt
     2341 cccaaagcac tccccaacca gaatgtgccg ctctcatctc ggaaaatccc ccgaatgttt
     2401 ccgcctacca aaccttcatt gtcagaaaaa cttctgaatt cattattact aaatcgtgtc
     2461 aatccatctc tgtttccaat ccacaaaaca ccggaacgat cggcatacag tgaggttacc
     2521 ttgttcgaca acaatccgtc acttcagccc ggatactatt cagggcgctt ttgctcaaaa
     2581 acccaagcgt gggatgttct aagtggggtg gggcgatcca ttggatcgcc tcgttagacc
     2641 ggctttagga actggcgcac ggcatccacc acccggcgcg gcgactcatc cagcacctgg
     2701 tgggaaagcc ccgccagttc tagaagttct gcctggggca gatgctcggc agcgtattgg
     2761 ccctcttccg ggggaacggt ctggtctcgg gagccgacga tcaccagtgt aggttgcttg
     2821 atctctgcca ggtgcggccc caggttttca cgggcgatac tgcggattcc atgcaccgct
     2881 gcccgccaat gagaccgctt gacatcctgc cggatacgca ggatcgagtg ttcgtctgcc
     2941 atacctggtc ggcctagcag cggcgcgatc acatccacca tatccgactg taccagcttc
     3001 cacagggctt ccgaacgctg aatagcgtag tcaccgacat ctgaagtgac cagcttgtct
     3061 agctccacca gcttaccaat gcgcccccgc acgacgggcg ctagcaatac cagttgatcc
     3121 atcaggtcgg gctgtgcctg tgctaatttg aggctgagca ttccgcccat ggaatgcccg
     3181 atgaccgccc ggggacgcag atgccgtcgt
//
LOCUS       DAOMYG010000383         3208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_11016_length_3208_cov_8.441801, whole genome shotgun sequence.
ACCESSION   DAOMYG010000383 DAOMYG010000000
VERSION     DAOMYG010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11016_length_3208_cov_8.441801"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="PK610_11340"
     CDS             complement(<1..1128)
                     /locus_tag="PK610_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRJ36577.1"
                     /translation="MNAKFFAGTLLTGFAGGMISFTAMEYFTDHKSETIIVEHRETKS
                     VPTSLTNQRPDIAPVLPVDFTYAAEQTVNSVVHIRTEVPAPRNAYVDPLYQYFHGPSP
                     RNNGFAEAAGSGVLLTADGYIVTNNHVIENARKISVTMNNNQTYTAKLVGADPGTDIA
                     VIRIEGKDLPAIEVGNSDDVKIGEWVLAVGNPFNLTSTVTAGIVSAKARNINILQGDP
                     SQEVYPVESFIQTDAAVNPGNSGGALVNAKGQLIGINTAIASNTGSYSGYSFAVPINL
                     VHKVTSDLIRFGVVQRGFLGVNIRNIDQQLATSLKLDRMNGVYVGGIVSGGAGEEAGI
                     SEGDIIVSVAGKDVSNVPELQEQVARYRPGDKIQIGVIRSRK"
     gene            1303..1989
                     /locus_tag="PK610_11345"
     CDS             1303..1989
                     /locus_tag="PK610_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36578.1"
                     /translation="MNKVPVFAAGILLGVGATAITVYLLNNPSVKTIDKSTPDVQTVS
                     SRSNPERSDTGSHTASTERSKPEYVRTKSDSDYFPNIDNNSALYDSLFAEQSKQEMEV
                     FDNGEVIRTDRMVAVKRIAVNDKTTEKSINAVDSVLNEANNMKEDQAAKNYEVEFWRS
                     ALNSRGYRMGKQKIALYGIDPQDQITLFRLEEGVFLRSGSHLYKLEVSPEFRAFRQVN
                     DKKILKEIEF"
     gene            1995..2762
                     /gene="dapF"
                     /locus_tag="PK610_11350"
     CDS             1995..2762
                     /gene="dapF"
                     /locus_tag="PK610_11350"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRJ36579.1"
                     /translation="MQFVKYQGTGNDFVMVDNRKTVFNRNDIARVQEICHRRFGIGAD
                     GLILIEPSSSADFEMVYFNSDGSMSFCGNGSRCAVAFAHELGITGTRCSFMAIDGLHE
                     AEVFANGIVGVSIRDVDVKSEYTDHQFIHTGSPHYIRFENELDHFPVVEEAKKIRYNA
                     NYKQEGTNVNFVQDLGDSIRVRTYERGVEDETLSCGSGVTACAISYAMKKGISDAVTI
                     QTEGGKLEVQFKRAANHFSGIRLIGPATRVFSGEING"
     gene            2755..>3208
                     /locus_tag="PK610_11355"
     CDS             2755..>3208
                     /locus_tag="PK610_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36580.1"
                     /translation="MADYRISNVRNPEPDDLLKGIWLMLYRPEQIPPHLILVIDGEYF
                     SLSVNGLKSREHISSLIRNALAKQHSILAIKVKFTGVPAQLQSEADGLFKKYSSPEEG
                     ITTCLYPIRDFFANHVSQDLKQAEFVFELYEKLNASGKTGKAIHYNLNH"
BASE COUNT          886 a          716 c          717 g          889 t
ORIGIN      
        1 cttcctgctg cggataacac caatctgaat tttatcgccg ggacgataac gtgcaacctg
       61 ttcctgcaat tcgggcacgt tgctgacatc cttacctgct actgaaacaa tgatatcacc
      121 ttcagaaatt ccggcctctt cgcctgctcc tccacttaca attccgccaa cataaacacc
      181 attcattctg tcgagcttga gtgaggttgc caactgctga tcgatgttgc gaatgttcac
      241 ccctaaaaat ccgcgctgta ccacaccaaa acgaatgaga tcgctggtta ctttgtgcac
      301 cagattgatc ggcactgcaa atgaatatcc cgaatacgaa cctgtattgg atgcaatcgc
      361 cgtattgatt ccgatcaact gccccttcgc atttacaagc gctccgccac tgttacccgg
      421 attcacggca gcatcggttt gaatgaagga ttcaaccgga taaacttcct gcgagggatc
      481 gccctgaaga atgttgatgt tccgcgcttt tgcacttaca attccggccg ttactgttga
      541 ggtgaggttg aacggatttc caaccgccaa aacccactcg cctatcttca cgtcgtcgga
      601 attacctacc tcaattgcag gtaaatcttt tccttcgatc cgtatcaccg caatatctgt
      661 tcctggatct gcccctacca attttgcagt ataggtttgg ttattgttca tggtgactga
      721 aatctttctt gcgttttcga tcacgtggtt attggtaaca atgtatccgt cggccgttag
      781 caaaactcct gatcctgctg cctctgcaaa tccgttgttg cgcggtgaag ggccatggaa
      841 atactggtat aaaggatcaa catatgcatt acgcggtgcg ggcacttctg tgcggatgtg
      901 cactaccgaa ttgaccgttt gctcagctgc ataggtaaaa tcaacgggca gaaccggagc
      961 gatgtcaggt cgttgatttg tcaatgaagt gggaacactt ttcgtttcgc ggtgttctac
     1021 aattatggtt tcgcttttat ggtcggtaaa atactccatt gcggtgaacg aaatcattcc
     1081 acctgcaaaa ccggtaagta atgtgcctgc aaaaaattta gcgttcatga tgatgtgttt
     1141 ttagtaccaa aggttattga caaaaaccgt gcattcgacg gttaataaaa aaacgttcaa
     1201 gcctcatttt agttattcgg gtggcgttaa aatgtgttaa tttttcagcg ggtttccaca
     1261 ctactaaacc ctaataaaat caagctattt gcgcctattt ttatgaataa ggtccccgtt
     1321 tttgctgcag gaattctgtt gggtgttggt gctaccgcca tcacggttta tttgttgaac
     1381 aatccgtcag tcaagacgat tgacaaatcg acacctgatg tacaaactgt cagctcccgg
     1441 tccaaccctg agcggtcaga taccggaagt catactgctt ctacagaaag aagtaaaccc
     1501 gagtacgttc gcacaaaatc cgattccgac tatttcccga atattgataa taacagcgct
     1561 ttgtacgaca gtctttttgc cgagcagtcg aaacaggaaa tggaggtttt tgataatggg
     1621 gaagtcatcc gaaccgatcg tatggtggcc gttaaacgta tcgccgtaaa cgataaaaca
     1681 actgaaaaat ccatcaatgc tgtagattct gttttgaatg aggccaacaa catgaaagaa
     1741 gaccaagctg caaaaaacta cgaagttgag ttttggcgct cggccttgaa ttcgagaggc
     1801 taccgcatgg gtaagcagaa aattgcactt tacggcatcg atcctcagga tcaaattact
     1861 ttgttcagac tggaagaagg cgtatttttg cgctccggat cacatcttta caaactggaa
     1921 gtctctcccg aattcagggc tttccgtcag gtaaacgata aaaagatcct gaaagaaata
     1981 gagttttgag cgcaatacaa tttgtaaaat accagggcac aggaaatgat tttgtaatgg
     2041 tggacaaccg taagacggtg ttcaaccgga atgatattgc ccgtgttcag gagatttgtc
     2101 accgtagatt tggtattggt gctgacggac tcatcctcat tgagccgagt tcctcggccg
     2161 attttgaaat ggtgtatttc aattccgatg gcagcatgag tttttgcgga aacggaagtc
     2221 gttgcgctgt tgcatttgcc catgaacttg gaataaccgg cacccgttgc agcttcatgg
     2281 cgattgatgg tctgcacgag gcagaagttt ttgcaaacgg tatcgtaggc gtgagcatac
     2341 gcgatgtgga tgtgaaatcg gaatacaccg atcatcagtt tatccatacc ggttcgccgc
     2401 attatattcg cttcgaaaac gaattggacc attttccggt tgtggaagaa gcaaaaaaaa
     2461 tcagatataa tgctaactac aaacaagaag gcaccaatgt gaattttgtg caggatcttg
     2521 gtgattccat tcgtgtgcgt acctacgaac gtggagtgga agatgaaaca ctttcctgtg
     2581 gttccggtgt tacggcctgt gcgatcagtt atgctatgaa aaagggaatt tcagatgcag
     2641 taaccattca aaccgaaggc ggaaaactcg aagtacagtt taaacgagct gccaatcact
     2701 tttcaggcat ccgacttatt ggtccggcca ctcgtgtatt ttcaggagag atcaatggct
     2761 gattatcgca tatccaatgt acggaatccg gaaccggatg atctgctgaa aggtatttgg
     2821 ctgatgcttt accgtcctga acaaattcct cctcatctca ttcttgtaat tgatggggaa
     2881 tatttttctt tgtctgtaaa cggattaaaa tccagagaac acatcagcag tctgattcga
     2941 aatgcactcg caaaacagca ttccattctc gccattaaag taaaatttac cggtgtccct
     3001 gctcaattac aaagtgaagc ggatggacta tttaaaaaat acagtagtcc cgaagaagga
     3061 attacaacct gcctctaccc aatccgcgat ttttttgcca atcatgtgag tcaggatttg
     3121 aagcaggctg aatttgtatt tgagctttac gaaaaactga acgcaagcgg aaaaacaggg
     3181 aaggcaatcc actataatct gaatcatc
//
LOCUS       DAOMYG010000384         3200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_11054_length_3200_cov_7.991415, whole genome shotgun sequence.
ACCESSION   DAOMYG010000384 DAOMYG010000000
VERSION     DAOMYG010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11054_length_3200_cov_7.991415"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PK610_11360"
     CDS             complement(<1..731)
                     /locus_tag="PK610_11360"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRJ36581.1"
                     /translation="MKTIKHLAFLFLILLNCSAFAQTGIKGVVKDKITGETLIGANVI
                     IDSIQPSVGAPTQLDGDYIINIKPGTYTIRCSFISYKTKVVTGVKVVEGKYTTVDFSL
                     EQAGGTELFEIDVVEARVNNTENAVLMDIKKANQVAVGVSSQEIAKSNFRDASEVARR
                     LPGVTVIDNRFVMVRGLSERYNEVWVNNGTTPSVEPDVKAFSFDLIPSNAVDRFLIYK
                     TGAPELPGNFAGGAVKIFTKDMPER"
     gene            960..1643
                     /locus_tag="PK610_11365"
     CDS             960..1643
                     /locus_tag="PK610_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ36582.1"
                     /translation="MSKEKTQRILLVDDEADIIELLKYNLEKEGYDVSTASNGREAIE
                     VAKKTKPHLILMDVMMPGMDGVEACMSLRDMDEFKTTIIAFLTARGEDYSQIAGFDSG
                     ADDYITKPIKPRVLISRIKALLRRGSTLNVASPESLSGISVDRERYLVFKDGEEMSLP
                     KKEFELLALLLSKPGKVFTREAILNEVWGNDIVVGDRTIDVHIRKLREKLGEDLIRTV
                     KGVGYKLEN"
     gene            1724..2689
                     /locus_tag="PK610_11370"
     CDS             1724..2689
                     /locus_tag="PK610_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ36583.1"
                     /translation="MVYIYSSQWEFVYPFVASVLVFIVSFVFIFYLVDRFIYARVKVI
                     YKSIYNQKIGKELKEKIQRSEDMIADVDKEVMGWAEDKEKEIEKLKEQESFRREFLGN
                     LAHELKTPIFSIQGYILTLLDGGLEDPSINRNFLQRAANGVDRITHIIEDLDMINQLE
                     SGKVELDIRKVNIIELCKEVMDSLEMKARERSISLNFNKSYDKPIFVNCDKGRIAQVL
                     TNLLVNSVNYGKEGGKTNIRFFDVDDKVLIEVADNGPGIPSEHLPRIFERFYRVDKSR
                     ARNQGGSGLGLSIVKHIVEAHGQALNVRSTENVGTTFAFTLERTR"
     gene            2797..>3200
                     /locus_tag="PK610_11375"
     CDS             2797..>3200
                     /locus_tag="PK610_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36584.1"
                     /translation="MKYVFLLFVFLILSCSQREEIKLHEDSWYHSLHGMQKEYFRAEY
                     TGLRKRMEKFRKPLVKKMNGSEKKKWIDRSREYLYTTLSDSLFQFWYGTPWDFNGITE
                     IPGEGNIACGYFVTTTLLHAGFELDRVRLAQQA"
BASE COUNT          920 a          579 c          697 g         1004 t
ORIGIN      
        1 cttctttccg gcatatcttt ggtgaaaatc tttaccgcac ctccggcaaa atttccaggt
       61 aattccggtg ctcctgtttt ataaatgaga aagcggtcaa ccgcattact tggaataaga
      121 tcgaatgaaa acgcttttac atctggctct actgaaggtg ttgttccgtt gttgacccat
      181 acttcattat agcgttcgct taatccgcgt accataacaa aacgattgtc aattacagta
      241 acaccgggta atcgtcgtgc aacttctgat gcatctctga agttggattt tgcaatttcc
      301 tggcttgaaa cacccacagc aacctgattg gcttttttga tgtccattaa gactgcattt
      361 tcagtgttgt ttactcttgc ttcaaccaca tcgatttcaa acaattcggt tcctcctgcc
      421 tgctcaagtg aaaaatcaac agttgtatat tttccttcta ccacctttac cccggttact
      481 actttggttt tgtagctaat aaatgaacac ctaatggtat aagttccggg tttaatattg
      541 atgatataat ctccatcaag ttgagtaggt gctcctacag agggttgaat ggaatcgata
      601 attacatttg cgccaattag tgtttctccg gttattttgt ctttcacaac tcctttaata
      661 cctgtttgcg caaacgctga acagttcagt aggatcagga ataagaatgc aagatgttta
      721 atggtcttca cagtttcaat tttatatctg caaaggtcag caccacacct tatggggttg
      781 ttaggtctgt gttatgtaaa ctttaactgc gcttttgggt aatgtggctg aatcccttta
      841 aaatagaggg attttgtctt tttggatgac ttaatatttt cgtaacttta atcctgaatt
      901 aatccgctat taacagcttt ttcgccttca gcccttaact ttggaaaaaa ttataatgaa
      961 tgtctaaaga aaaaacacaa cgaatattgt tggtcgacga tgaggcggat atcattgaat
     1021 tgttgaaata caatctcgaa aaggaaggtt atgatgtttc cacagcatct aatggcaggg
     1081 aggcaattga agtagcaaaa aaaaccaagc cccatttaat cttgatggat gtgatgatgc
     1141 ccggcatgga tggcgttgag gcttgtatga gcttgcggga catggatgag tttaaaacaa
     1201 ccatcattgc ttttcttact gccagggggg aagattattc tcagatagca ggatttgatt
     1261 ccggagcgga tgattacatt accaagccca ttaagccaag ggtacttatc agccgtatta
     1321 aggccttgct tcggagagga tctaccttaa atgttgcttc acccgaaagt ttgtcaggta
     1381 tcagtgtaga ccgcgagcgt tatttggtgt ttaaggatgg cgaggagatg tcgctgccta
     1441 aaaaagagtt tgaattactt gcattgctgc tttccaagcc cggaaaagtt tttactcgtg
     1501 aggccatttt aaatgaagtt tgggggaatg atattgtggt tggagatcgc actattgatg
     1561 ttcacatccg aaaattacgt gaaaaactcg gagaagattt aattcgcact gttaaaggtg
     1621 ttggatacaa gttggaaaat tgatgaatac atcaacaccc aggggcattg ctttttttgt
     1681 atcgctgata cttgggatgc tttccttttt aattgttatg gtaatggtgt acatctactc
     1741 ttctcagtgg gagtttgtat acccatttgt agcatccgta ctcgtattta tagtctcatt
     1801 cgtttttatt ttttacctgg tggatcgatt catttacgca cgcgtaaaag tgatctacaa
     1861 atccatttat aatcagaaga tcggaaaaga gttaaaggaa aaaattcagc gctctgagga
     1921 tatgattgct gatgtagata aggaagtaat gggctgggca gaagataagg agaaggaaat
     1981 tgaaaaatta aaggaacagg agagtttcag acgcgaattc cttggtaacc tggcgcacga
     2041 attgaaaact cccatattca gtattcaggg ttatattctc actttgttgg atggtggact
     2101 cgaagatcct tccatcaatc gcaattttct tcaaagagca gctaacggtg tggaccgtat
     2161 tacgcacatc attgaagatc tggacatgat caatcagctt gagtctggca aggtggaact
     2221 tgatattcgt aaggtgaata ttatcgaact ctgtaaagaa gtgatggatt cactcgaaat
     2281 gaaagcacgt gaacgaagta tttccctaaa ctttaataag agttacgaca aaccaatttt
     2341 cgttaattgc gataagggaa gaattgcaca ggtattgacc aacctccttg tgaattccgt
     2401 taactacgga aaagaaggtg gaaaaacgaa tattcgtttt tttgatgttg atgataaagt
     2461 tttaattgaa gttgctgata atggtcccgg aattccatct gaacacctgc cacgaatatt
     2521 tgaacgtttc tatagagtag ataaaagtcg cgcccgcaat cagggggggt caggactagg
     2581 actttcaatc gtaaaacaca ttgttgaggc tcatggtcag gctctgaatg tgaggagtac
     2641 cgaaaatgtt gggactacct ttgcatttac gctcgagcgt acccgctaaa ttttccttat
     2701 aactttttct gcggtcaata gaccggttac cgcacataat cattggttgc tttggttgat
     2761 tatgatggtt ttagatttac agaaatcatt ttctgtatga agtatgtatt tctattattt
     2821 gtgtttctga ttctttcctg ttcccaaagg gaagaaatta aattgcatga agactcatgg
     2881 tatcatagtt tgcatggcat gcaaaaggaa tatttccgtg ctgaatacac cggacttcgt
     2941 aaaaggatgg aaaagttccg taaaccgctt gtaaagaaaa tgaatggtag tgaaaagaaa
     3001 aaatggatcg atcgatcccg cgaatacttg tacaccacac tcagcgattc actatttcaa
     3061 ttttggtatg gtactccatg ggattttaac ggtataacag agattccggg cgaaggcaat
     3121 attgcttgtg gatattttgt taccacaact ctgcttcatg ccgggtttga attggatcgg
     3181 gtccgattgg cgcaacaagc
//
LOCUS       DAOMYG010000385         3173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_11205_length_3173_cov_10.971777, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000385 DAOMYG010000000
VERSION     DAOMYG010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11205_length_3173_cov_10.971777"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PK610_11380"
     CDS             <1..321
                     /locus_tag="PK610_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36585.1"
                     /translation="ELEQRYQHILSNLEKDLGEKPDMQTVIFLIGVQELNKGYQKLSK
                     QEKLEVMHIGVCVLLSPYGFYSPLGRDEEGWPHFESKKKLPPLDGRQQQYLLKQAIVD
                     YFTE"
     gene            complement(311..685)
                     /locus_tag="PK610_11385"
     CDS             complement(311..685)
                     /locus_tag="PK610_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36586.1"
                     /translation="MRNKLFSGLFIVLVALAVVSCGKSGDTTATITVLDVNNQPVPGA
                     LVRVVGYDSNGGCNSTPCRIDRETETNGSGQATFNFNDLYKRGAAGFAVLEVEASKNG
                     MTGDGIIKVEEQVNNEAKVSIQ"
     gene            742..1575
                     /locus_tag="PK610_11390"
     CDS             742..1575
                     /locus_tag="PK610_11390"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRJ36587.1"
                     /translation="MKQVIHTCLFVSALIFSSFTLFKAPSGTVKIKVETPMGNIVAVL
                     YDETPLHRDNFLKNIKEGVYNNLLFHRCIQEFMIQGGDPGSREAKPGQVLGNGGLNYT
                     VPAEFRKEHFHKKGALAAARMGDDVNPHKASSSTQFYIVQGKVFEDNVLTMMEERNNQ
                     QIRNAIFNEILQRPENAEMLKEAIAASQKGDQAKIQEIVTKLTPAIEEEFAKRKFSFS
                     PEQRKAYTTTGGAPHLDGAYTVFGEVIEGLDVIDKIAAEKTDGNSRPLNDIRFSISIV
                     Q"
     gene            1572..2594
                     /gene="pheS"
                     /locus_tag="PK610_11395"
     CDS             1572..2594
                     /gene="pheS"
                     /locus_tag="PK610_11395"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRJ36588.1"
                     /translation="MKETIEKLLSEVEQLSAANAEEAEQHRIRLLGKKGVITALFDAF
                     KTVGPEQKKEFGKILNELKNKASEKIEVLKSSTASTAASHSEGDLTRPGEPLPLGARH
                     PLSLVRKEIDAIFTRLGFVIADGPEIEDDWHNFGALNFPENHPARDMQDTFFIETAER
                     NIALRTHTSSVQVRMMENNKPPLRMIMPGRVYRNEAISARAHCFFHQVEGLYIDENVS
                     FADLKQTLMYFAQEMFGEKTKIRLRPSYFPFTEPSAEMDVSCSLCAGEGCNVCKHSGW
                     LEIMGCGMVDPAVLENCGIDSKKYSGFAFGMGVERIAQLKYQVKDLRLYSENDVRFLK
                     QFTSVV"
     gene            2600..2944
                     /locus_tag="PK610_11400"
     CDS             2600..2944
                     /locus_tag="PK610_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36589.1"
                     /translation="MKQFLFFKGTAIAAFHPDEMDPPFGSGHVDLIPADNTIISGAQE
                     YLQFTIKEFELSDTSKAYAQFEKALECDYADWYLSTEWSIGEKAEGAYSILQPMMSRE
                     GYLVFTVGEGGY"
BASE COUNT          963 a          657 c          765 g          788 t
ORIGIN      
        1 gaacttgaac aacgctacca acatatactg agcaaccttg aaaaagacct gggtgaaaag
       61 cccgatatgc aaacggtcat ctttctgatt ggcgtgcagg agctcaacaa aggctatcaa
      121 aaactaagca aacaggaaaa actggaagta atgcacattg gtgtttgtgt gctgctaagt
      181 ccgtatggat tttattctcc cttgggacga gacgaagaag gatggccgca ttttgagagc
      241 aaaaaaaagc tgcccccctt agacggaagg cagcaacaat atctgctcaa acaagcgatt
      301 gtcgactatt ttactgaata gaaactttcg cttcgttgtt gacttgttct tcaactttga
      361 taatgccgtc gccggtcata ccattctttg atgcttccac ctcaagcact gcaaaacctg
      421 cagcaccgcg cttgtaaaga tcattgaaat taaaggtagc ctggccactt ccgttggtct
      481 cggtctcacg atcgatacgg cagggagtgc tgttgcaacc tccgttggaa tcatagccca
      541 caacacgaac aagtgcaccc ggcaccggtt ggttgttcac atctaatact gtaatggtag
      601 cagtggtatc acccgacttc ccacaagaaa cgactgcaag tgctactagc actataaaga
      661 gtccggaaaa cagcttgtta cgcataggtc cgatttttgt aaatttgtac gctatctatc
      721 aaaaatagca aaaataagga tatgaaacaa gttattcaca cctgtttatt tgtcagcgcg
      781 ttgatattca gctcgtttac actgtttaaa gccccttcgg gaacggtaaa aatcaaggtt
      841 gaaaccccaa tgggcaacat tgtggcggtg ctctatgatg aaacaccctt gcatcgcgac
      901 aactttctaa agaacatcaa agaaggcgtg tacaacaatt tactctttca ccgctgcatt
      961 caggaattta tgattcaggg aggagatccc ggctccagag aagccaaacc gggacaggtt
     1021 cttggtaacg gcggattgaa ttacacggtg cctgcagaat tccgcaaaga gcatttccac
     1081 aaaaaaggcg cgcttgctgc tgcgcggatg ggtgatgatg tgaatccaca taaagcttcc
     1141 tcttctacgc aattttacat tgtacagggg aaagtgtttg aagacaatgt actcaccatg
     1201 atggaagagc gcaacaatca gcaaatccgg aatgctattt ttaacgaaat actccagcgt
     1261 ccggaaaatg ctgaaatgct gaaagaagcc attgctgcat cgcaaaaagg agatcaggca
     1321 aaaattcagg aaatcgttac caaacttact ccggccatcg aggaagaatt tgcaaaacgt
     1381 aaattcagct tttcacctga acaacgcaaa gcctacacca caaccggagg cgctccacat
     1441 ttggacggtg cttacactgt tttcggtgag gtgattgagg gtcttgatgt gattgacaaa
     1501 atcgcagcag aaaaaactga cggtaatagt cgtccgctta atgatattcg tttttcaatc
     1561 agcattgtgc aatgaaagaa accatagaaa aattactcag cgaagttgaa caactcagcg
     1621 ctgccaatgc agaagaagcg gaacaacacc gtattcgttt actcggaaaa aaaggagtga
     1681 tcactgcgct cttcgatgcg tttaaaacgg taggacccga acagaaaaaa gaattcggaa
     1741 aaattctgaa tgaattaaaa aacaaggctt cagaaaaaat tgaggtctta aagtcatcca
     1801 ccgcttcaac agcagcatcc cacagcgaag gcgatttaac gcgtcccggc gaaccccttc
     1861 cccttggagc gcgtcaccca ttatcattgg tacgcaaaga gatcgatgcg atttttacgc
     1921 gacttggatt tgtgattgct gatggtccgg aaatagaaga cgactggcac aatttcggcg
     1981 cattgaattt tccggagaac cacccggcgc gcgacatgca ggatactttt tttattgaga
     2041 ccgccgaacg taatattgca ttacgcactc atacatcgag tgtacaggtc cgcatgatgg
     2101 aaaacaacaa accacccttg cgcatgatca tgcccggacg tgtatatcgc aacgaagcaa
     2161 tttctgcgcg tgcgcattgc ttttttcatc aggtggaagg attgtatatc gacgagaatg
     2221 tttcgtttgc tgatcttaaa caaacgctga tgtattttgc acaggaaatg tttggagaaa
     2281 aaacaaaaat acgtttgcgt ccttcgtatt tcccctttac cgaacccagt gcggaaatgg
     2341 atgtatcttg ctccttgtgc gcaggcgaag gatgcaatgt atgtaagcac agcggctggc
     2401 tcgagatcat gggctgcggc atggtagatc ctgcggtact cgaaaactgt ggtatcgaca
     2461 gcaagaaata ttcgggcttt gcatttggaa tgggcgtaga acgtatagca caattgaaat
     2521 accaggtgaa agaccttcgc ttgtattcgg aaaatgatgt gcgttttcta aaacaattta
     2581 cgtctgtagt ataatcgtat tgaagcagtt tttatttttt aaaggaacag cgatcgcagc
     2641 atttcatccc gatgagatgg atcctccttt tgggagtggt catgtcgatt taattcctgc
     2701 agacaacacc atcatctccg gcgcgcagga atatttacaa tttaccatta aggaatttga
     2761 attgagcgat acaagtaaag cttatgctca atttgagaaa gcgctcgaat gcgattatgc
     2821 cgattggtat ttatcgaccg aatggagtat tggagaaaaa gcagaagggg cgtattccat
     2881 tttgcaaccg atgatgagca gggaggggta tttggtgttt acggttggag agggaggcta
     2941 ttgagggaat gtgataatgt gctaatgagg gatgggctaa tgggggaatg tgataatgtg
     3001 ttagtgtgct aatgggataa tgagataatg ggggaatgaa ggagggggag atggaaaggg
     3061 aagcgcgaag tgagggaggg agaacgagga agaggacgaa cgctaagatg gacaggcccc
     3121 aaatagctga agatttcctg aattaacgca tactctatca tacaaatagg cat
//
LOCUS       DAOMYG010000386         3166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_11245_length_3166_cov_7.629380, whole genome shotgun sequence.
ACCESSION   DAOMYG010000386 DAOMYG010000000
VERSION     DAOMYG010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11245_length_3166_cov_7.629380"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..171)
                     /locus_tag="PK610_11405"
                     /pseudo
     CDS             complement(10..171)
                     /locus_tag="PK610_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009090500.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            421..1995
                     /gene="bshC"
                     /locus_tag="PK610_11410"
     CDS             421..1995
                     /gene="bshC"
                     /locus_tag="PK610_11410"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HRJ36590.1"
                     /translation="MQTASIPTFNHLSSVWKTVKSIEPDKAELLLADSILGKIAERQS
                     FEHREILVSALHRQYGSEIGKTISSQLDLLKKNNAFTVTTGHQIGLLGGPAFFFYKII
                     TTIRLAEELRRKSPDHIFVPVLWMATEDHDKAEISSVHVFGKELVWETNQEGAVGRFS
                     TDDLQQYIESVEALKGSLPHGDSIMEIFKKAYAPGKTLANATREWVHSFFSDFGLVII
                     DGDDPELKRLFRQVMETEILHSPTEKIVIDRNAELEKGGLNAQVHPREINLFYLSANN
                     RKRIVKTADGFAEHEGTMHWTQESIMAEIGAHPERFSPNVLLRPLYQEIILPNLAYVG
                     GPGELSYWMQLEGVFSHFAVSEPICFPRNSIMLIDQGQQQRIGKLSLQVQDLFRSADE
                     LVNEFLKENAGAEMDLEAEKKKIEEQFSLIAEKTSGIDATLKSMVMAELQKTLKSIEQ
                     IEGRIRKSEKAKHETSLNQIRTLKEKLFPGGNPQERVESLLPHLWKHGNGLLKDIHAQ
                     TNPFDLRYHVLFPDSP"
     gene            complement(1975..>3166)
                     /gene="hutI"
                     /locus_tag="PK610_11415"
     CDS             complement(1975..>3166)
                     /gene="hutI"
                     /locus_tag="PK610_11415"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006990112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HRJ36591.1"
                     /translation="PEMKILPVIENAWLAIEDGIIADYGSMNDWPGISDWRDLEVVDA
                     DGRIVLPAWIDSHTHLVYAGSREGEFVDRINGLSYEDIAARGGGILNSSKRLAETGEE
                     ELYESAMQRLMQLQNMGTGAVEIKSGYGLTLETELKILRVIRRLKSTTDLTIKSTFLG
                     AHAFPLAYKENKQGYIDHLINEMLPAVAAEKLADYCDVFCERNYFSEEQTITILEAAK
                     KHGMIPKVHANQLSRSGGVQAGVKVGAVSVDHLEYVDQEEIAALRGSKTMATLLPGAQ
                     WFLSLPHPPARRMIDSGLAVAIASDYNPGSSPSGNMALMLSMACVQYNMTPEEAINAA
                     TINAAYAMGISSESGSISPGKIANLIITEKIPGYAFIPYAFGMNVNAAVMIKGKIVSG
                     TIGK"
BASE COUNT          909 a          626 c          672 g          959 t
ORIGIN      
        1 aattttttat ttaagtgttt ttttttgttg ttttttaggt aagtttatta ccatagttgt
       61 taaaacagct gcattgtcaa ccgacaacag gctttctatc aggataagat taagaataat
      121 gagaccggcg gcttcaaggt cgggaccaag aatgctgtgg aggaagtcca tgcaagaaag
      181 ttggaatgta aaagtagtaa ttagttgtca gtttggcgat actagggggg gcataagttg
      241 agatttcctt gcaatcaatt taaattggta attggtaatt ggtaattggt aattgggtaa
      301 ttaggtaatt aggtaattgg caatcggatt ataggtcctg aagttgaaat atccttgcaa
      361 tcaatccaca tcaagatcgt tgtattgaat tggctattct gcttttattt acatttgttt
      421 atgcaaacag cttcaattcc aacatttaac catttgtcat cggtttggaa aacggtaaag
      481 tcgatcgaac ctgataaggc tgagttatta cttgctgatt ctattctggg aaaaattgct
      541 gagcgacaat ctttcgaaca ccgcgagatt cttgtttccg cattgcaccg tcagtatggt
      601 tctgaaatcg gaaaaaccat atcatcacaa cttgatttgc tgaagaagaa taacgcgttt
      661 acagtaacaa ccggacatca gatcggatta ttgggagggc ctgccttttt cttttataaa
      721 atcattacca ccatcagact tgcagaggaa ttacgcagaa agagtcctga tcatattttt
      781 gttcccgttt tatggatggc tacagaagat catgacaaag cagaaatttc ttccgttcat
      841 gtttttggaa aagaattggt ttgggaaaca aatcaggaag gtgctgttgg acggttttcc
      901 acagatgatc ttcagcaata tattgaatcc gttgaagcgt taaaaggtag cctgccacat
      961 ggcgattcta ttatggagat cttcaaaaaa gcctatgctc caggaaagac gcttgcaaat
     1021 gctaccagag aatgggtgca ttcgtttttt tctgattttg gtttagtcat cattgatggc
     1081 gatgatccgg aattgaaacg gttgtttcga caggttatgg agacggagat tcttcattct
     1141 ccaaccgaaa aaattgtaat agatcggaat gcggaattgg aaaagggtgg attaaatgca
     1201 caggttcatc cacgggaaat caacttgttt tatttgtctg caaacaacag aaaacgaatt
     1261 gtaaaaaccg ccgatggatt tgccgaacac gaagggacta tgcattggac acaggaatcg
     1321 atcatggccg aaatcggagc acatccagag cgattttctc caaatgtttt gctgcgtcct
     1381 ctttaccagg aaatcattct tcccaatctc gcttatgttg gtggaccggg tgaattatca
     1441 tattggatgc agttggaagg ggtgttttct cattttgcag taagtgaacc aatttgtttt
     1501 ccgcgcaatt ctatcatgct aattgatcag ggacaacagc aacgtatagg taaattgtcg
     1561 cttcaggtac aagatctttt tcgttctgca gatgaacttg tcaatgaatt tctgaaggaa
     1621 aatgctggtg ctgaaatgga tcttgaagca gaaaagaaga aaattgaaga acagttttca
     1681 ttgattgcgg aaaaaacatc aggtattgat gccactttga agtcaatggt aatggctgaa
     1741 ctgcaaaaaa ccttaaaatc gatcgagcag atcgaaggcc gtatacgcaa gtcggaaaag
     1801 gcaaaacatg aaaccagtct gaatcagata cgcacactta aagaaaaatt atttcccggc
     1861 ggaaatcctc aggagcgggt tgaatcattg ttgccccatc tttggaaaca tggtaatggc
     1921 ttacttaagg atattcatgc gcaaaccaat cctttcgact tgcgctatca tgttttattt
     1981 cccgatagtc cctgatacaa tttttccttt gatcattacc gccgcattaa cgttcattcc
     2041 gaatgcgtaa ggaataaagg catatcccgg aatcttttct gtgattatca aattagcaat
     2101 tttacccgga ctgatactac ctgattcaga tgaaattccc attgcataag ctgcgttaat
     2161 tgtcgctgcg tttatggctt cttcaggagt catgttgtat tgcacgcaag ccatcgacaa
     2221 cattaaggcc atattacccg aaggagagct tccgggatta taatcgctcg caattgcaac
     2281 tgcaagtcca gaatcaatca ttcttcgcgc cggtgggtgt gggagtgaga gaaaccattg
     2341 ggctccaggt aatagtgttg ccattgtttt acttcctctt aatgcagcaa tctcttcctg
     2401 atctacgtat tcgagatgat caacactaac tgctcctact tttacgcctg cctgtacacc
     2461 tccggagcga cttaactggt tggcatggac ttttggaatc atgccgtgtt tttttgcggc
     2521 ttcgagaatg gtgatcgttt gttcctcgga aaaataattt ctttcgcaaa acacatcgca
     2581 ataatccgca agtttttcag cagcaacagc aggcaacatt tcattgatga gatgatcaat
     2641 gtaaccctgt ttgttttcct tgtatgccaa aggaaatgcg tgcgcaccca gaaatgttga
     2701 ttttatggtc agatctgtag ttgatttcaa gcgacgaatc acacggagta ttttaagttc
     2761 tgtttcgagc gttaagccat atccgctttt gatctccaca gcacctgttc ccatgttttg
     2821 taattgcatt aatcgctgca ttgccgattc atataactct tcttcaccgg tttcagcaag
     2881 tcgtttcgaa gaattcaaaa taccaccacc acgagcagca atatcttcat agcttaatcc
     2941 attgatgcga tcaacaaatt ctccttcacg acttcctgcg tatacaagat gggtgtgcga
     3001 atcgatccat gcaggtaaaa caatacgacc atctgcatcc actacttcaa gatcacgcca
     3061 atccgaaatt ccgggccagt cgttcatact tccatagtcg gcaataatcc catcttcaat
     3121 agcgagccag gcgttttcaa ttaccggtaa gattttcatc tcgggt
//
LOCUS       DAOMYG010000387         3120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_11442_length_3120_cov_11.901142, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000387 DAOMYG010000000
VERSION     DAOMYG010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11442_length_3120_cov_11.901142"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..775
                     /locus_tag="PK610_11420"
     CDS             98..775
                     /locus_tag="PK610_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ36592.1"
                     /translation="MVRVVIADSNELIRIGLRTILERQPDVEVVDEAINRDALKEAII
                     RFEPHVVLIDHSSPGFSIDCVPEILQHNKKVRFVAITQNHTAKTIENALKSGISSYIK
                     KDCDMMEIVDSVTETARGKKFFCGTILEMLREEGIDLDQIGAEPLSCAPVSLSDREME
                     IIGLIAEGYTNQQIAEKLFLSSHTINTHRKNIMAKLGINNTAGIVIYAVKCNLVSPNK
                     YLFSAQN"
     gene            complement(846..>3120)
                     /locus_tag="PK610_11425"
     CDS             complement(846..>3120)
                     /locus_tag="PK610_11425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ36593.1"
                     /translation="YDLHLKTTTDGWMYLPNAVKVVSSIYSYSYVYPPQCHNDVNGSI
                     DISAMGGSQMLSYLWSTGATTSSVNNLPAGSYNIVVTDDITGCTYEESFILDNPPPMT
                     VSVHKVDSDCGLENGYAWVVVNGGIPPYDIYWPNEVSGDTLFNIIGNSSFHVEVNDEN
                     NCYFFLPVNVYDQLTLNVTTTPVLCGSLDGQATVSIANGIAPYSILWSNGDTGSSADS
                     LTTGQHIVQVTDANSCYGSLSFQVAGSNGPQVTYSVTPISCESQPNGAIDLTVTGGQA
                     PYTYLWSNGTTTQDIAGLSPGTYQVVVRDASGCSYAECIEVSSSNAFALYWYSTSTPT
                     CLMNDGSIELYPEGGVLPYNVQWSANAGIQSGTVATNLAAGFYYAIVTDAAGCTDSLM
                     TSLSNYDAPYFYWDNSNEGTCGANDAYIEITPAGGAGFGYNTIWDNTNMVEDLYNTNP
                     GSHYLSISDAFGCYGNSIVELGSVQPAIQEICLVTVDSLTGKNLVVWEKATLGNISHY
                     NIYRETCEPSGYAFVGMVPYDSLSQFLDLGADPFVQSWKYKMTAVSDCGVESQFSAMH
                     KTIHLYADRTLSTTVDLSWDNYIGFSFTEQIIWRHHQSTGWQIIDTVSSTVFTYTDLT
                     PPSVDSSEYFIEVYSPVACVSTRAINHNSTRSNRGTVAAPNDNSVNENNILGNMSVYP
                     NPSAGLFNLAMDIHNERTVTVRVFDLSGKLILSREINVVNGLNNYMIDMGTASNGLYQ
                     VVVSGSSSKMATRIIKQ"
BASE COUNT          920 a          622 c          689 g          889 t
ORIGIN      
        1 aagcagtacg agcaccgtga cattttttga ggtttttgct ttttgtattg ttgcagggct
       61 tacttttgag tgttatttaa aatcaatcta aataaacatg gtaagggttg tgattgctga
      121 cagtaatgag cttatccgga ttggacttcg taccattctg gagaggcaac ccgatgttga
      181 agtggttgat gaagccatta acagagatgc gcttaaagaa gccatcatcc gatttgaacc
      241 tcatgttgta ctgatcgatc attcaagtcc gggattttca attgattgtg ttcctgagat
      301 tttgcagcac aataaaaaag taaggtttgt cgcaattacg caaaaccaca ctgccaaaac
      361 catcgaaaac gcattgaagt cggggatcag cagttatatc aaaaaggatt gcgacatgat
      421 ggagattgtt gattccgtta cagaaacggc aagagggaaa aagtttttct gtggtaccat
      481 tctcgaaatg ctgcgtgaag aaggaattga ccttgatcag atcggagctg aaccattgag
      541 ttgtgcgccg gtgtcattga gtgatcgcga aatggaaatt attggattga ttgcggaggg
      601 atataccaat caacaaatcg ccgaaaaatt gtttttgtcg agccatacca ttaacacgca
      661 ccgaaaaaat ataatggcaa aactgggtat caacaatacc gcaggaattg tgatttatgc
      721 ggttaaatgc aatctcgttt cacccaataa atacttgttt tctgcccaaa attgatgttt
      781 ggttaggttt gaaaaagaaa agacccggaa tttcccgggt ctttttttat tgatggtgaa
      841 tgatgttatt gtttgatgat gcgggttgcc attttactgg atgatccact taccacaacc
      901 tgatatagtc cgttggatgc agtacccata tcgatcatgt aattgttgag tccatttacc
      961 acattaattt cacggctgag gatcaatttc cctgagagat cgaatactcg aacagtcact
     1021 gttctttcat tgtgaatatc catagcaagg ttgaataatc ccgcagaagg attaggatat
     1081 acactcatgt ttccaaggat attgttttca ttgacgctgt tatcattggg cgcagcaact
     1141 gttccacgat tagagcgggt ggaattgtga ttgatcgcac gggtagatac acaggcaacc
     1201 ggcgaataaa cttcaatgaa atattcagaa gaatcgacac ttggaggagt tagatctgta
     1261 taggtaaata ctgtggatga aacagtatcg ataatctgcc atcctgtgct ttggtgatgt
     1321 ctccaaatga tctgctcggt aaagctgaat ccaatgtaat tgtcccagga aagatcaaca
     1381 gttgtagaca atgttctgtc agcatacagg tgaatggttt tatgcattgc actaaattgt
     1441 gattcaacac cacagtcgga aactgcagtc attttgtatt tccatgactg aacaaaagga
     1501 tcggcaccta aatccaaaaa ctgactcaga ctgtcataag gcaccattcc cacgaaagca
     1561 tatccactcg gctcgcaagt ttcacggtag atgttgtaat gagaaatatt gccaagtgtt
     1621 gctttttccc agacaacaag attttttccg gtaaggctat caactgtaac aagacaaatt
     1681 tcctggattg caggctgaac acttcccagc tcaacgatag aattgccata acagccaaac
     1741 gcatcagaga tggataaata gtgtgatccc ggattggtgt tgtaaagatc ttcaaccatg
     1801 ttggtgttgt cccaaatagt attgtatcca aatcctgcac cacctgctgg tgtaatttct
     1861 atgtatgcat cgttagcgcc acaggttcct tcattagaat tgtcccaata gaagtaggga
     1921 gcatcgtaat tggaaagtga tgtcatcaat gaatccgtac aaccagcagc atctgttaca
     1981 atagcgtaat aaaaacctgc agccagattg gttgcaactg tacccgactg gattccggca
     2041 ttggcactcc attgaacgtt atagggtaac actccccctt cagggtaaag ttcgatcgat
     2101 ccgtcgttca ttaaacaagt gggtgtagat gtagaatacc aatacaaagc aaaagcattt
     2161 gaactgctta cttcaataca ctctgcataa ctgcatccac tggcatctct tacaactacc
     2221 tgatacgttc cggggctaag tcctgctata tcctgagttg ttgttccgtt ggaccataaa
     2281 tacgtgtagg gtgcttgtcc gccggtaact gttaaatcaa ttgctccgtt gggttgagat
     2341 tcgcaactga tgggtgttac actgtaggta acttgcggac cattacttcc ggcaacctga
     2401 aaagacaagc ttccatagca ggaattggca tctgtaactt gaacaatatg ctgaccggta
     2461 gttagactgt ctgcagaaga accggtgtct ccattactcc ataaaatcga gtagggagca
     2521 attccgtttg cgatgcttac tgttgcctga ccatcaagac ttccgcataa aacaggagtg
     2581 gtggtaacat tcagtgtgag ctgatcataa acattcaccg gtaagaagaa gtagcagttg
     2641 ttttcatcat tgacttcgac atgaaaagat gaatttccga tgatattgaa taaggtatca
     2701 ccagacactt cgtttggcca gtaaatatca taaggaggaa tccctccatt tacaacgacc
     2761 catgcatatc cgttttccaa tccgcaatcg gagtcaacct tatgcactga aacagtcatt
     2821 ggaggaggat tgtccaaaat gaaagattct tcataggtac aacctgtgat gtcatcagtt
     2881 acaacaatat tgtacgatcc ggcaggcagg ttgttgacac tggaagtggt tgctcctgtg
     2941 ctccataagt agctgagcat ttgtgaaccg cccattgcac tgatatcgat gcttccgttt
     3001 acgtcgttat gacattgagg aggatataca taggagtagg aataaatgga ggaaactacc
     3061 ttgaccgcat ttggcaagta catccaacca tcggttgttg tctttaagtg aagatcgtaa
//
LOCUS       DAOMYG010000388         3096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_11569_length_3096_cov_7.035843, whole genome shotgun sequence.
ACCESSION   DAOMYG010000388 DAOMYG010000000
VERSION     DAOMYG010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11569_length_3096_cov_7.035843"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PK610_11430"
     CDS             complement(<1..865)
                     /locus_tag="PK610_11430"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HRJ36594.1"
                     /translation="MFHFLRLIRPLNLLIIAFTMYMMRYFVVGGKWMDWQGNETSDLF
                     CFTMTHFDFFLLVISTMSVAGAGYIINDYFDIRIDRINKPEKVIVGKYIKKRVAMLWH
                     TLLNFFAIGIAVYLTFKYYNFWPLIFHLSTTLGLWMYSLSLKRQFLSGNLTISLMAGM
                     VVLLSGLADLPDMMLRYQEVPEGYPFTLEQLSFSWKAIWVFTGFAFLTTLIREVQKDL
                     ADVKGDEAQKCKTVPIVWGIPKTKKLVIALVVLLLVPLFLIVFSLQLELYRLIFISFA
                     VIVPILYSGFLT"
     gene            complement(956..1708)
                     /locus_tag="PK610_11435"
     CDS             complement(956..1708)
                     /locus_tag="PK610_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36595.1"
                     /translation="MRKSILLLALAIVISSTISAQRWLDIGFKGAWGPTWLMNKNLMN
                     DQQFNHVLAYGYGIGGKVGFNFNDEHELTLDVMASTFKQDFKYNVTDTVSGASPEFKK
                     GMQFKTLDFILMYRHNKDGRYFEVGPGYSTIRSASGTNERPEFATLDGEISENLIKGY
                     SILALGFGGYFVGTENFGITMGARFTYGLSDLISEKGKQNNYPAHYPGTTRYTTYTQS
                     HPFTAMLVMEANLDFAYMVKAKCSNKRKILLF"
     gene            1769..2515
                     /gene="fabG"
                     /locus_tag="PK610_11440"
     CDS             1769..2515
                     /gene="fabG"
                     /locus_tag="PK610_11440"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015782609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRJ36596.1"
                     /translation="MKILQGKVALITGASRGIGRGIALRFAQEGANIAFTYASSEEKA
                     KALENELSALGIKAKGYKSDAANFDQAMQLVDDVVKEFGTLDVLVNNAGITRDTLLMR
                     MTEQQWDEVINTNLKSVFNLTKAAQKPMLKQRNGSIINMSSVVGVKGNAGQSNYSASK
                     AGIIGFTKSIALELGSRNIRSNAIAPGFIETEMTEVLDEKVVQGWRDSIPLKRGGTTE
                     DVANACLFLASDLSSYITGQTIHVCGGMLT"
     gene            2532..>3096
                     /locus_tag="PK610_11445"
     CDS             2532..>3096
                     /locus_tag="PK610_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36597.1"
                     /translation="MKIILSYPLWYIVFCLSAGGLYAAILYFREKQLSEIRTWLKALI
                     ATTRFVVISLLSFFLLQPLIRTEKRRVEKPLIVIAQDNSGSVIIGKDSSFYKGEYLAE
                     IDRLKSKLEENYDVRSYTIGNKVREGLNIDYTDQSTDLGGLFEELFTRYYNRNLGAVI
                     LATDGIFNQGTSPLYSAKRLKGVPVFTI"
BASE COUNT          985 a          648 c          632 g          831 t
ORIGIN      
        1 aggtgagaaa accactataa agaatcggga cgatcactgc gaaacttata aagattaacc
       61 ggtacaattc cagctgaaga gaaaatacaa taagaaaaag gggtacaagc aaaagaacaa
      121 ccagagctat aaccagtttt ttcgttttgg gaattcccca aacaattgga accgttttac
      181 atttctgtgc ttcatctcct ttcacatccg caaggtcttt ttgtacttca cgaatgagcg
      241 ttgtgagaaa tgcaaatcct gtgaataccc aaattgcctt ccatgagaac gacaattgtt
      301 ccaatgtaaa aggatatcct tccggaactt cctggtacct cagcatcatg tcgggaagat
      361 ctgccagacc ggagagcaaa acaaccattc ccgccattaa ggaaatagta aggttgcccg
      421 aaagaaattg ccgcttcagg gacaatgaat acatccataa cccaagcgta gtggacaaat
      481 gaaaaatcaa tggccagaaa ttgtagtatt taaaagtgag gtaaacagca attccaattg
      541 caaaaaaatt cagaagggta tgccaaagca tggcaactcg ttttttgatg tattttccta
      601 caataacctt ctccggctta ttgatgcgat cgattcggat atcaaaataa tcattgataa
      661 tgtatccggc gccggcaaca gacattgttg atatcacgag taaaaagaaa tcaaaatgcg
      721 tcattgtaaa acaaaagaga tcggaggttt catttccctg ccaatccatc catttaccac
      781 caaccacaaa gtaccgcatc atatacatgg taaatgcaat gatgagtaga ttcaggggtc
      841 gtataagtcg tagaaaatga aacattcgcc gggctaatat attacatacc gattaattca
      901 cccacacaaa aacatacaca ataaaaaacc ccggttgttc cggggtcatt atcgtttaaa
      961 aaagaagaat ctttcgcttg ttcgaacact tggcttttac catataagca aaatcgagat
     1021 tcgcttccat taccagcatc gctgtaaagg ggtgcgactg cgtataggtt gtataacgtg
     1081 ttgttccagg ataatgcgcc ggataattgt tttgcttccc cttttcgctg ataagatcac
     1141 tcaggccata ggtgaatcgt gcgcccatgg taatcccgaa attttcggta cccacaaaat
     1201 aaccgccaaa gccaagcgcc aggatcgaat atcctttaat taaattctca ctgatctctc
     1261 cgtcgagtgt agcaaattcc ggacgctcat tagtgcctga tgcacttctg atagtggagt
     1321 atccaggacc aacttcaaag taacgaccat ctttattatg acggtacatc agaataaaat
     1381 caagtgtttt gaattgcatg ccctttttaa attcgggact tgcaccggaa actgtatctg
     1441 ttacattgta tttaaaatcc tgcttaaatg ttgatgccat gacatcaagt gtaagctcgt
     1501 gctcatcatt gaaattgaat cctacttttc caccaattcc ataaccatag gccaaaacat
     1561 ggttaaattg ctgatcattc atcaggtttt tattcatcag ccaggttgga ccccatgcac
     1621 ctttgaaacc tatatccagc cagcgctgcg cagagattgt tgaacttatt acaatggcca
     1681 gtgccaacag gaggatagat tttctcatta tctttggttt tcggaaagaa tcaaaattat
     1741 ccaatttcag caaccaaaca atacatctat gaaaatcttg caaggaaaag tcgcattaat
     1801 taccggagcc tcgagaggaa ttgggagagg cattgctcta cggtttgctc aggaaggtgc
     1861 taacatcgca ttcacctatg cgtcctcaga agaaaaagca aaggctttgg aaaatgaact
     1921 tagcgctctc ggtatcaagg caaaggggta taaatctgat gcagcaaact ttgatcaggc
     1981 catgcaattg gtagatgatg tggtgaagga attcggaaca cttgatgttc ttgtcaataa
     2041 tgcaggaatt acccgcgaca cactattgat gagaatgacg gaacagcaat gggatgaggt
     2101 aattaacacc aatctgaaat ctgttttcaa tctcacaaaa gcagctcaga aacccatgct
     2161 taaacaacgt aacggatcga taatcaacat gtcatccgtt gttggagtga aaggaaatgc
     2221 aggacagtcc aattattcag cttcaaaagc cggaattatt ggattcacaa agtcaatcgc
     2281 ccttgaattg ggttcaagaa atatccgaag caacgccatt gctcccggtt ttatcgagac
     2341 agaaatgacg gaagtgctgg acgaaaaggt ggttcaggga tggagagatt cgattcctct
     2401 taaaagaggc ggaaccactg aggatgttgc caatgcatgt ttattcctcg cctccgatct
     2461 ttcttcgtac atcaccggac aaacaattca tgtttgtggt gggatgctta cctgattcct
     2521 gatccataac cttgaaaata atactcagtt atcccctatg gtacattgtc ttctgtcttt
     2581 cggccggagg actttatgct gccattctct atttcagaga aaagcaactg agtgaaatac
     2641 gtacctggct taaggcattg attgcaacaa cacgatttgt tgtgatcagt ctgctctcat
     2701 tttttctgct tcaacccctt attcgaacag aaaaacgaag ggtggaaaaa ccactcattg
     2761 taattgcgca ggataattcg ggatctgtaa tcattggaaa ggactcttcg ttttacaaag
     2821 gagaatacct cgcagaaatc gaccgactaa agagcaagct cgaggaaaat tatgatgtcc
     2881 gctcttatac tattggtaac aaggtaaggg aaggattaaa catcgactat accgatcaga
     2941 gtacagattt aggtggattg tttgaggaat tgtttacgcg gtactacaac aggaatcttg
     3001 gtgcagtaat tctcgccacc gatggcatat tcaatcaggg cacatcaccg ttatacagtg
     3061 caaaaagact gaaaggagtt cctgtattta ctattg
//
LOCUS       DAOMYG010000389         3082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_11629_length_3082_cov_9.828543, whole genome shotgun sequence.
ACCESSION   DAOMYG010000389 DAOMYG010000000
VERSION     DAOMYG010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11629_length_3082_cov_9.828543"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1828)
                     /locus_tag="PK610_11450"
     CDS             complement(<1..1828)
                     /locus_tag="PK610_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36598.1"
                     /translation="MRAYFFCFIWLCLGCLNGFSQQTYVPFPCRVLSQQDVQTTGNNL
                     LIRQENCIETSGNILVNNPESVLLESGQSIHLKPGFKAAPGTAEQFNARINRSEVGLV
                     IYEPAQTPGQVPVFEKFELGVDLPDTILQLVERYVAQPRQYPNINPFNPDEISIEAQF
                     FILNENGQFHPFPDGMWMGPEIKYGFYYEDFQRDSASWTKLSNQHPFRIRFASSWVGN
                     WKVKIFVNVQGLGSYESSEYSFISTPSPNPGFLKVHPINKQYLTFSGRNESFFPVGQN
                     LFFPVYLDPAAGITFGGEPYDADPAKPRAYRYYLAELDTFAANGANYFRMLNTPWTTE
                     IEYEHVNDYSGRMSNAWEMDQILNKCKEKDLYLHWNLSIHYVLENPSHYSMYNWDWDD
                     GPLLPGVCDHTTPGYCYKTELGLSDPGEFFTDPEALKNYKHRLRYYISRYSYSTNIAV
                     LELFSEINNAGTVTEVVPPKPAEQIFWCHSNNNTLYSPYENEAPFRADIASWHNIMSN
                     YIRNGLNHWHQILTANYAGVPKNDDHSYSLPYIDMVTYNSYSRLPSRIEGYSTTASNV
                     HQHYNKPLLHAESGAPEVDPCGNYAEWIKTAWISAFSGHAGTA"
     gene            complement(1833..>3082)
                     /locus_tag="PK610_11455"
     CDS             complement(1833..>3082)
                     /locus_tag="PK610_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36599.1"
                     /translation="RNDICGMGAPLYIQSNGASSHTIFNYGNATNVGIGTNAPQYKLH
                     VVGNTSFDGGVRLPQLAGSGGWLGVDAQGNLVSRSLEMAGFDIYSLPCLTDNNGNFFP
                     VWGNGSGILYASNNNCGNVIKVGINTDNPLYQLDVRGDAFVSETLRVGNNELFLGGQN
                     QSGSRNVIYSNGNLLLQSTPGYNFNTLINANNTGNVGIGTNSPVSRFQVGDGVEAFSV
                     GSAYFPGLGYGTSYIGFNLRRGTTNWETNAGISHNGGGIIYASVLGNFHFISIPSTGS
                     VAQTGLTDQDVLDNTQVMVYSDGRMGIGTQCVPYDHRLAVDGKLICEEVTVKMSDAQG
                     CWPDYVFSEEYKLMPLPEVETYIAQNGHLPNMPSAAVVEEGLELKSIVVKQQQTIEEL
                     MLYILELNRRLKELEESAANGKQ"
BASE COUNT          899 a          710 c          594 g          879 t
ORIGIN      
        1 atgccgtacc tgcatggccg gaaaaagcag aaatccatgc cgtttttatc cattctgcat
       61 aatttccaca agggtctact tccggagcac cggattcagc atgcaacagt ggcttgttat
      121 agtgttgatg aacattacta gcagtagtag aatacccctc aattcgtgaa ggcagccttg
      181 aataactatt ataggtcacc atatcaatat aaggaagcga ataactatga tcatcatttt
      241 taggtactcc cgcataatta gctgtaagaa tctgatgcca atgatttaat ccattcctga
      301 tataattcga cataatatta tgccacgaag cgatatcagc tctaaaagga gcctcatttt
      361 cataaggtga gtataaagta ttattgtttg aatgacacca gaatatttgt tctgcaggtt
      421 ttggaggcac aacctctgtt accgttcctg cattgttgat ctcactgaag agttccaaaa
      481 cagcaatatt ggtgctgtat gaatagcgcg atatataata ccttaagcgg tgcttgtagt
      541 ttttcaatgc ttccggatcg gtaaaaaact cacccggatc agatagtccc aattcggttt
      601 tgtaacaata gcccggagtg gtatgatcac aaaccccggg taacagtggg ccatcgtccc
      661 aatcccaatt gtacatggag taatgcgatg ggttttccaa tacgtaatga atcgataagt
      721 tccagtggag gtataaatct ttttccttgc acttgtttaa aatctgatcc atctcccagg
      781 cattgctcat acgacccgaa taatcattga cgtgttcgta ttcaatttca gtagtccatg
      841 gtgtattgag cattcggaaa tagtttgcac cgttggcagc aaatgtatcc agttcagcta
      901 agtaataacg atatgctctt ggctttgcag gatcagcatc ataaggctcg ccaccaaagg
      961 taattccagc agcagggtca aggtaaacag ggaaaaacaa attctgtccg acagggaaaa
     1021 atgattcatt tcgtccagaa aaagttaaat actgtttatt tattgggtgt acttttaaaa
     1081 agccagggtt aggagaaggg gtgctaataa atgaatattc acttgattca taacttccaa
     1141 gaccctgaac atttacaaat atttttactt tccagttgcc cacccatgag gatgcaaagc
     1201 gtatcctaaa aggatgttga ttagaaagct ttgtccaact cgcagaatca cgttgaaaat
     1261 cttcatagta aaatccatat ttaatttctg ggcccatcca cattccatca ggaaatgggt
     1321 gaaattgtcc gttttcatta agaataaaaa attgggcttc tatactgatc tcatccggat
     1381 taaatggatt aatattggga tattgccgcg gctgtgcaac ataccgttcc accaattgca
     1441 aaatagtatc cggtaaatca acccccaatt cgaatttttc aaagaccgga acctgacccg
     1501 gtgtttgtgc tggttcgtaa atgaccaagc caacctcact gcggttaatg cgggcattga
     1561 actgttctgc ggttccggga gcggccttaa atcccggttt taagtggatg gattgtcccg
     1621 attccaataa aacagattcc ggattgttga ccaggatatt tccactggtt tcaatacaat
     1681 tctcctgtct gatcaataaa ttatttccgg tagtttgaac atcctgctga gaaagaactc
     1741 tgcacggaaa aggcacatag gtttgctgag agaagccatt gagacaaccc aaacaaagcc
     1801 aaataaaaca gaaaaaatac gctctcatcg tcttattgct ttccgtttgc tgctgattct
     1861 tccaattcct tgagccgtct gttcaactcc aaaatataca acattaattc ctcaatggtc
     1921 tgttgttgtt ttactacgat actttttaat tccaaccctt cctctaccac tgcagcagaa
     1981 ggcatattgg gtagatgccc attctgcgca atataggttt ccacttccgg taaaggcatg
     2041 agcttgtact cctccgaaaa cacataatcc ggccaacaac cctgggcgtc gctcatctta
     2101 acagttactt cctcgcaaat caactttccg tcaacagcaa gacgatgatc ataaggcacg
     2161 cactgagttc caattcccat tcgaccgtcg ctataaacca tcacctgggt attatcaagc
     2221 acatcctgat cggtcaatcc ggtttgtgca acagaacctg tggatggtat gcttataaaa
     2281 tgaaaattcc ctaaaactga tgcataaatt attccaccac cattatgact tattcctgca
     2341 tttgtttccc agttggtagt tccgcgtcga aggttaaagc caatatatga tgttccgtaa
     2401 cctaatccgg gaaaataggc ggatcccaca gaaaaggcct ctaccccatc acccacctgg
     2461 aatcgggaaa ccggagaatt tgttcctata ccaacatttc ctgtgttgtt ggcattgata
     2521 agcgtattga aattatatcc gggcgtggat tgaagtaata aattcccatt ggaataaatt
     2581 acatttctgc tgcctgattg attttgtcca cctaaaaaaa gttcattgtt tcccacgcgc
     2641 aaagtctccg aaacaaaagc atcaccacgc acatccaact ggtacaatgg attgtccgta
     2701 ttaatcccta ccttaattac atttccgcaa ttgttgttgg atgcatacaa aatccccgaa
     2761 ccattgcccc ataccggaaa aaagttacca ttattatctg ttaaacacgg caaggaataa
     2821 atatcaaatc ctgccatctc caggctccgg ctaaccaagt taccctgcgc atccactccc
     2881 aaccatccac cagatcccgc cagttgaggt aagcgtactc cgccatcaaa actggtgtta
     2941 cccactacgt gcagtttgta ctgcggggcg ttggtgccta tcccaacatt ggtggcgttt
     3001 ccgtaattaa aaatggtatg cgagctcgct ccgttgctct gtatatacaa tggtgcgccc
     3061 attccgcaaa tgtcgtttcg gc
//
LOCUS       DAOMYG010000390         3081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_11635_length_3081_cov_7.803040, whole genome shotgun sequence.
ACCESSION   DAOMYG010000390 DAOMYG010000000
VERSION     DAOMYG010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11635_length_3081_cov_7.803040"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="PK610_11460"
     CDS             <1..1161
                     /locus_tag="PK610_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRJ36600.1"
                     /translation="RTKEMSPVELVNILDQFFAGFDRVITRFQLEKIKTIGDAYMAVG
                     GVPEKRKENPVLTTLAALEIARQMDQHWRVQNKLGKQHWELRIGIHTGPLVAGVIGKK
                     KFAFDIWGDTVNIASRICSHCDPGKVNISAATYEKIKDYFECEYRGEIEGKNVGHVGM
                     YFVKGLKPEFSLGGNGRKPNRAMKQIAGLIFIQFDRLLETILQRLENELPANLYYHGV
                     HHTRDVLQAVQAIGREEELSPDEQLMLNTAALLHDCGYLYTYSHNEELAVKMARKILP
                     DFGYTSAQIKIISGIIRTTKIRSVPKTKLQKIMNDADYDYLGRKDYTRIAESLRLELE
                     EQGRPFEEKEWISLQIYFLKKHQFYTTTASKLREDQKQANIVKLEKAMLRLL"
     gene            complement(1164..>3081)
                     /gene="priA"
                     /locus_tag="PK610_11465"
     CDS             complement(1164..>3081)
                     /gene="priA"
                     /locus_tag="PK610_11465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRJ36601.1"
                     /translation="YETMIELSAACTDEDALREVFAQLEKDKRAQKRVDALLQFIQLS
                     GRYSTDPKSVKKSELVKALGGNSSPIDTLIKAGVFEIRQVRVDRLAPEKSATSTLPVL
                     SDEQQRAFDEISKGFNENKPVLLHGVTGSGKTEIYIRLIQEILEKTDGQVLYLLPEIA
                     LTTQIIQRLRRFFGDMVGIYHSRFNDQERAEIWAKTLKGEYRILLGARSSVFLPFTNL
                     KMIIVDEEHENTFKQFDPAPRYHARDAGIVLAARNNAKILLGSATPSIESYHNADTGK
                     YVLVELKKRFGGILLPEIQCVDIKQAHKRKEMHSSFSHDLLEHIKVALGNKEQIILFQ
                     NRRGYTPQWNCETCSWVPRCKNCDVSLTYHKSTHQLICHYCATAYPPPKTCSACGSHK
                     LKMVGFGTEKIEEDLELLIPGIRIQRLDLDSTRTKNAYVQILTDFEERNIDVLVGTQM
                     VTKGLDFDNVALVGILNADTMLNFPDFRAFERSYQLLSQVAGRAGRKGKRGKVLIQTW
                     EPNHWVIQKVMQNDYIGMYKQEIIERKNFHYPPFIKLIKITLRNKDKTKVDEAAIKLA
                     SALKKVMGTRVLGPEEPSIPRIRNYFYRTILLKIERGATYPQWKKQLMEWLLEFRKEY
                     DKTRTLIDLDVDPF"
BASE COUNT          853 a          613 c          651 g          964 t
ORIGIN      
        1 cgtaccaaag agatgtcgcc tgtagagctg gtaaatattt tagatcagtt ttttgctgga
       61 ttcgacagag taattacccg ttttcagctt gaaaaaatca agacgatcgg ggatgcgtac
      121 atggcagtag gaggagtgcc cgaaaagcga aaggagaatc cggttttaac tacattggca
      181 gctcttgaaa ttgcgcgcca aatggatcag cactggagag tacaaaacaa attgggcaaa
      241 caacattggg aattaagaat cggcattcat acaggtccct tggttgcggg tgtaattggg
      301 aaaaagaagt ttgcattcga tatatgggga gacactgtaa atattgcatc ccgaatttgt
      361 tcgcattgtg atcccggaaa agtgaatatt tcggcagcta cctacgaaaa gataaaggat
      421 tattttgaat gcgaatacag aggagaaatt gaagggaaaa atgtgggtca cgttggcatg
      481 tatttcgtta agggattaaa accggaattt tcgctcggcg gaaacggtag aaaacccaat
      541 cgggccatga agcagattgc aggactgatt tttattcagt ttgatcgttt gctggaaact
      601 attttgcagc gtctggaaaa tgaattacct gcaaatcttt attatcatgg ggttcaccat
      661 acccgcgatg tgttgcaggc tgtacaagct attgggcggg aagaggaatt atcgccggat
      721 gagcaattga tgctgaacac agctgccttg ttgcacgatt gtggttattt atacacctat
      781 tcgcacaacg aagaactagc tgtaaaaatg gctcgtaaaa ttttaccgga ttttgggtat
      841 acgtcggcgc agatcaaaat tatttccgga attatccgta caacaaaaat aagatctgtt
      901 cccaaaacca agctgcagaa aattatgaat gatgcagatt atgactattt aggaaggaag
      961 gattataccc gcatcgctga atctttacgt ctggaactgg aagaacaggg taggccattc
     1021 gaagaaaaag aatggatcag tttacaaatc tatttcctga aaaagcatca gttttatact
     1081 acaactgcat ccaaacttcg cgaagatcag aagcaagcca atatcgttaa gctggagaaa
     1141 gcgatgttac gattgttatg atgctaaaaa ggatcaacat ccaggtcaat taacgttctg
     1201 gttttgtcgt attcctttct gaattccaat aaccattcca ttagttgttt tttccattga
     1261 ggataggttg ctcctctctc aatttttaag agaattgttc ggtaaaagta attcctgata
     1321 cggggaatgg aaggttcttc cggacctaat acccttgttc ccattacttt tttcagcgca
     1381 gaagccaact taattgcagc ttcatcaacc tttgttttgt ctttatttct taatgttatt
     1441 ttgatcagtt ttataaacgg gggataatgg aaattctttc gttcaataat ttcctgtttg
     1501 tacatcccta tgtaatcgtt ttgcattact ttctggatca cccagtgatt cggttcccag
     1561 gtttgaataa ggacttttcc acgtttgccc tttcgtcccg ctcttcctgc tacctgcgat
     1621 aatagttgat acgaacgttc gaatgctctg aaatccggaa aattcaacat ggtgtctgca
     1681 ttcagaattc ctaccaaagc aacattatcg aaatccagac cttttgttac catttgtgtt
     1741 cctaccaaga catcaatatt tcgctcttcg aaatcggtta ggatttgtac gtaagcattt
     1801 tttgttcgtg tagaatccag atcgagtcgt tgtatacgga ttccgggaat cagtaattcg
     1861 agatcttctt caattttttc ggtaccaaat cctaccattt ttaacttatg actaccacag
     1921 gctgagcagg tttttggtgg gggatatgcc gttgcgcagt agtggcaaat cagttggtgt
     1981 gtactcttgt ggtaggtcag gctgacatca caattcttac atcgcggtac ccatgaacag
     2041 gtttcgcagt tccattgagg agtatatcct ctcctgtttt gaaacagaat gatttgttcc
     2101 ttgtttccaa gagcaacttt tatgtgttcc agcagatcat gcgaaaagga ggagtgcatt
     2161 tcttttcgtt tgtgagcctg tttaatatcg acacattgaa tttccggtag taatattcct
     2221 ccaaaccgtt ttttcaactc taccagtaca tattttcctg tatcggcatt gtggtaactc
     2281 tctattgagg gtgttgccga acccagcaga atttttgcat tatttcgtgc ggccagaaca
     2341 attcctgcat ctcttgcatg ataccttgga gcaggatcga attgtttgaa tgtgttttca
     2401 tgttcttcat ccacaataat cattttaaga tttgtaaatg gaagaaaaac agaggatcgt
     2461 gctcctaata aaattctgta ctcgccttta agtgttttag cccagatctc agctctttcc
     2521 tgatcgttga accgtgaatg gtaaatgcct accatgtccc caaaaaagcg acgtaaccgc
     2581 tgaatgattt gtgttgttaa tgcaatttcc ggaagcaaat acaatacctg tccatccgtt
     2641 ttttccagta tttcctgaat gagacggata tagatttcag tttttcccga acctgttaca
     2701 ccatgcagca acaccggttt gttttcatta aatcccttgc tgatttcatc aaatgcacgc
     2761 tgttgttcgt cggataaaac gggtagggta gaagtcgctg atttttccgg agccaaacga
     2821 tcaaccctga cctgccgaat ttcaaaaaca ccggccttga tcagggtatc gatcggggaa
     2881 ctatttccac ccaatgcctt tacgagttcg gattttttta cgctttttgg atcagtggaa
     2941 tagcgtccgg atagttgaat aaattgcaaa agtgcatcca cccgtttttg tgctctttta
     3001 tccttttcaa gttgagcaaa aacttccctt aatgcatctt catctgtgca ggccgcagat
     3061 aactcgatca ttgtttcgta c
//
LOCUS       DAOMYG010000391         3075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_11674_length_3075_cov_8.417219, whole genome shotgun sequence.
ACCESSION   DAOMYG010000391 DAOMYG010000000
VERSION     DAOMYG010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11674_length_3075_cov_8.417219"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1677)
                     /locus_tag="PK610_11470"
     CDS             complement(<1..1677)
                     /locus_tag="PK610_11470"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRJ36602.1"
                     /translation="MTKHLRHILLLFTTIVLSAHFAHTQTTRVSGKVIDAETKEPLPG
                     VHIVFTGTKIGCVSDPDGNYLLESYYSSDSVTATFIGYKRLSKSIKKDKINIVNFEMV
                     SDDNILDEIIVKAPDVDPAVLIFKMIIKNKPVNDREKLQSYEYNVYNKVEFDLNNFDE
                     KFMNRKILRPISFIFDNIDSTNEKPYLPLFITESVSDYYFRKNPKSNKEFIHATKVSG
                     IENESVSQFLGDMYQNINIYDNIISIFGKSFVSPISDRGMLYYDYYLLDSAFVEDDWC
                     YKVRFVPKRKQEPTFVGQFWVNDTTFAIRKVEAGLADNAPINFITYFEFVQEFKQVEK
                     EVWMLSRDYLLIDFKLIDSKKNMGFYGRKTTSYKNHVINKPKESSFYSGPNNIVVADD
                     ADEKDNNYWLNARHDSLTEAEAAIYQLMDTIQEIPQIKSTINLVSMLVNGYKVIGPVE
                     WGPYFTTASFNRFEGPRLRFGGRTSNKFSKRLELNAYVAYGFRDEKWKYSGGFRYMIS
                     KKPHRQVLSFQYKYDLEQLGISGNAFRSDNLLASVFRINPANKLSMVEEYK"
     gene            complement(1722..2069)
                     /locus_tag="PK610_11475"
     CDS             complement(1722..2069)
                     /locus_tag="PK610_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRJ36603.1"
                     /translation="MSNPIDEFNDYRSRMNERLLANDNLVIKRFFNLDTNCYSEGILP
                     VKTKEMLGLVASAVLRCDDCIKYHLEKCYKEGITREEMLEILQVALVVGGSITIPHMR
                     RAYEFWEELEKRA"
     gene            complement(2066..2878)
                     /gene="tatC"
                     /locus_tag="PK610_11480"
     CDS             complement(2066..2878)
                     /gene="tatC"
                     /locus_tag="PK610_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRJ36604.1"
                     /translation="MTADTKEMSFFGHLEELRKRLFRMAAAVILVAVVLFFYTEWIID
                     TVYIAMSKPNFITYRAFCKIGKLLDLQDTLCANEIKLQFQSIKMTGQFSTNIYFSFVG
                     GIVLSFPYLFLQVWGFVKPGLKKNETSATKGIVLWASLLFLCGIFFGYFIIAPLTVQF
                     FGNYTMSQDIMNIPTIGDYIGIITATTFWTGLLFELPVVIYILTKIGIVGPEFLRTYR
                     KHAIVVVLILSAVITPPDFFSQIIVSIPILILYEISIFVSARTEKARIKSKA"
BASE COUNT          893 a          618 c          599 g          965 t
ORIGIN      
        1 tttgtattct tccaccatcg aaagtttgtt cgccggattt attctgaata cagaagcaag
       61 taaattgtcg gaacggaatg catttcctga aatacctaat tgttccaaat cgtatttgta
      121 ttgaaaactc aatacctgtc gatgaggttt tttagaaatc atgtaacgaa aacctccgct
      181 gtatttccat ttttcgtctc tgaaaccata agcgacatat gcgttcaact ctagtcgttt
      241 gctgaactta tttgatgttc ttcccccaaa tcgaagtcgt ggtccttcaa agcggttaaa
      301 gcttgcagta gtaaaatagg gtccccattc caccggtccg atcactttat atccgtttac
      361 aagcatgctc actaaattga tcgttgattt gatttgcggg atttcctgaa tggtatccat
      421 caactggtaa attgctgctt ctgcttcagt gagggaatcg tgacgtgcat tgagccaata
      481 attattgtcc ttttcgtctg catcgtctgc aacaacaatg ttgtttggac cactgtaaaa
      541 gctactctct tttggcttgt tgatcacatg gtttttgtac gatgtcgttt tcctgccata
      601 aaagcccatg ttttttttac tgtcgatgag tttgaaatca atcaacaaat aatccctgct
      661 aagcatccag acttccttct caacttgctt gaattcctgt acaaattcga agtaggtaat
      721 aaaattgatt ggcgcattat ctgcaagccc ggcttccact tttcgtattg caaacgtggt
      781 gtcgttaacc caaaattgcc caacaaaagt gggttcctgt ttccgtttcg gtacaaatcg
      841 tactttataa caccaatcgt cctcgacgaa tgcagaatct aaaaggtaat aatcataata
      901 aagcattccg cggtctgaga tcggactcac aaaactcttt ccaaaaatgc taatgatgtt
      961 gtcgtagata tttatgtttt gatacatgtc gcccaggaat tgggaaacac tttcattctc
     1021 gatacctgat acttttgtgg catgaatgaa ctccttattc gattttgggt tttttctgaa
     1081 gtaataatca gatacagatt ccgtaataaa cagtggcaaa tagggttttt cgttggtaga
     1141 atctatgttg tcgaaaatga aacttatcgg acgaagaatt tttctgttca tgaatttctc
     1201 atcgaaattg tttagatcaa attcaacttt attgtacaca ttgtattcgt agctctgtag
     1261 tttttccctg tcgtttacag gtttgttttt gataatcatt ttaaaaatca aaactgcagg
     1321 atccacatcc ggcgctttta caatgatttc gtcaagaata ttgtcgtccg ataccatttc
     1381 aaaattgaca atattgattt tgtctttttt aatggattta cttagccgtt tatagccgat
     1441 aaaagttgct gtaacggagt cggacgagta gtaggattcg agtaagtaat tgccgtccgg
     1501 atcgctaacg caaccaattt tcgttcccgt aaacacgata tgaacacccg gtaacggttc
     1561 ttttgtttct gcatcaataa ctttcccgct tacccttgtt gtttgcgtgt gcgcgaaatg
     1621 tgccgacaaa acaatggtcg tgaaaagtaa aagaatatgc ctcaggtgct tggtcaaatg
     1681 gagtatgatt cgtataaatt taatgaaaaa cagtttgttc cctatgccct tttttcaagc
     1741 tcttcccaaa actcatatgc ccttcgcatg tggggaattg taatagagcc gccaacaacc
     1801 agtgccacct gcaaaatttc cagcatttct tctcgggtaa tgccctcttt gtaacacttt
     1861 tcaagatggt acttaatgca gtcatcacaa cgcaaaacag cagaggctac aagtccgagc
     1921 atttcttttg tctttaccgg cagaatccct tctgaataac aattggtatc caggttgaag
     1981 aatcgtttta taacaagatt gtcatttgcc agcaggcgtt cattcatcct gcttcggtag
     2041 tcgttgaatt cgtcgatggg gttactcatg ccttactttt tatgcgcgct ttttctgttc
     2101 gtgcagaaac aaaaatgctg atctcataga gaatcagaat gggtatggaa acaataattt
     2161 gactaaagaa atcaggagga gtaattactg cagacagaat gagtacaaca acaatagcat
     2221 gcttgcgata agtacgaagg aattccgggc caactattcc aattttggta agaatgtaaa
     2281 tcaccaccgg taattcaaat agtaaacctg tccagaaggt tgttgcagta ataataccta
     2341 tgtaatcgcc gatggttgga atattcatga tgtcctgcga catggtgtaa tttccaaaga
     2401 actggacagt gagtggtgca ataatgaagt atccaaagaa aataccgcac aggaaaagaa
     2461 gcgatgccca aagaacaata cccttcgttg cagaggtttc attttttttc aatcccggtt
     2521 ttacaaatcc ccacacttgt aaaaagagat aggggaagga gagtacaatt ccgccaacaa
     2581 atgagaaata aatattagtg gaaaactgcc cggtcatttt aatgctctga aactgaagtt
     2641 tgatctcatt tgcgcaaagg gtgtcttgaa ggtccaataa cttcccgatt ttgcaaaacg
     2701 cacgataagt aataaaattg ggtttcgaca tcgctatata aaccgtatcg ataatccatt
     2761 ccgtatagaa gaagagaacc actgcaacaa gtattacggc agcagccatt ctgaaaagtc
     2821 gcttgcgcaa ttcttccagg tggccaaaaa aggacatttc tttagtgtcg gctgtcataa
     2881 gcggcaaaaa tagcacaaaa ggccaagggc tcagcgatta aaataccaat tgcggaactc
     2941 aatttgttcc tttgttaaaa cagggctctt cctcaggtaa tacgctttca tttctttttt
     3001 ggaatggggg cgtgcctgaa gaatttgatc tgctttatcc cgggtgattt ttagtctgat
     3061 ttcagcatta cttac
//
LOCUS       DAOMYG010000392         3069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_11703_length_3069_cov_8.918381, whole genome shotgun sequence.
ACCESSION   DAOMYG010000392 DAOMYG010000000
VERSION     DAOMYG010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11703_length_3069_cov_8.918381"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PK610_11485"
     CDS             complement(<1..312)
                     /locus_tag="PK610_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HRJ36605.1"
                     /translation="MKKKKELTRAEEEIMQILWKIESGFVKDILAEFSAPRPAYNTVS
                     TIVRILETKGFVKHKAHGKTHEYFPAVEKEEYLKSTVKSMMKLGDLHHQLLAGLDQLF
                     DL"
     gene            complement(391..1752)
                     /locus_tag="PK610_11490"
     CDS             complement(391..1752)
                     /locus_tag="PK610_11490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRJ36606.1"
                     /translation="MVYSFLKYLFRIAVKAFFRNKKVLGIENIPNSGPLLIVANHPST
                     FMDPIVIATVIPGEVNFIAKAEAFKSKFAQWLLPRFNMIPVYRKQYDPSLMHKNKDTF
                     RKVFDLLQSGGNVLIFPEGISITDRKLKEIKTGAARMALGAEEERDFRLNCRIVPVGL
                     TYSDPHKFQSDLVIRIGEPIEVRNYHDMYLSDEVQASKALTEEIRLCIESLVVDIQDE
                     AVDVFVSRVETIYKAKLLHEMGCSDRMIDKGLEVSKMITERIHYFMEQQPQRVEETKQ
                     KIDHYFQMMERLNLEDRIIRGTDRKLPVMLQALFWVFYFVIGFPLFVFGAINNYLPYK
                     IPYQIARLMKAEPQFVGAIMMVSGTFIFLLFYLVQIILMQHYFQDGMLTIAYAVLLPI
                     SGLFAFRYWRRFIHIRKRWYYFSLFLRRSRLIASMLVLREQILEDLERGRNEYDKLKG
                     NIR"
     gene            complement(1812..2828)
                     /locus_tag="PK610_11495"
     CDS             complement(1812..2828)
                     /locus_tag="PK610_11495"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRJ36607.1"
                     /translation="MREHHARHRSEKNFISLTEARSNKFPVEWNADDIHSPKKPGVQV
                     LNDFPLEKLVPYIDWTPFFQTWELAGRFPKILTDEVVGAEATKLFNDATALLDRIVTE
                     KWISANAVYGIFPANSNGKEDIEIYSDESRKQISAISHQLRQQTKKTQGAYNMCLADF
                     VAPKSTGLQDHIGAFVVTAGIGMEKWIQKFEKDHDDYNSIMLKALADRLAEAFAEYLH
                     EYVRKDAWAYAASEKLSNEELISEKYRGIRPAPGYPACPDHTEKLTIFQLLDAERRSG
                     VSLTESLAMYPAASVSGWYFAHPQSKYFGLGQIQKDQVEDLAKRKQEDYSMVEKWLSP
                     VLAY"
BASE COUNT          924 a          690 c          560 g          895 t
ORIGIN      
        1 aagatcaaag agctgatcaa gcccggccag gagctgatgg tgcaagtcac caagtttcat
       61 catagatttt acagttgatt tcaggtattc ctccttttcc accgccggaa aatattcatg
      121 tgtttttcca tgtgctttgt gcttaacaaa tcccttagtt tcaaggatgc gcacaatagt
      181 tgacaccgta ttataggcag ggcgaggagc agaaaattcg gcaagaatgt cttttacaaa
      241 tccggattct attttccata agatttgcat tatctcttct tcggcacgag taagttcttt
      301 ctttttcttc atggaaataa aattataact aaaatcctag ttaatagaac tatttatact
      361 ggaatgaaga aacggtcgct ctcaaacgga ttaacggata ttccccttca gtttatcgta
      421 ttcatttcgg cctcgctcga ggtcctcaag aatttgctca cgcaaaacca acatactggc
      481 aatcaggcga gagcgtcgca agaataatga aaaataatac caccttttac ggatatgaat
      541 aaacctgcgc cagtagcgga atgcaaataa gccactgatc ggaagaagga ccgcgtatgc
      601 gatagttagc ataccatctt gaaaataatg ttgcatcaaa atgatctgaa ctaaataaaa
      661 caatagaaaa ataaacgttc cgctcaccat catgatcgct ccaacaaatt gtggttctgc
      721 tttcatcaaa cgggcaattt ggtaaggaat cttgtaaggg agatagttgt tgatggcgcc
      781 aaatacaaat aatggaaatc ctattacaaa atagaacacc caaaacaaag cctgcagcat
      841 gacaggtaat ttacgatcag tacctcgaat aattctgtct tccagattca atcgctccat
      901 catctgaaaa tagtgatcga tcttttgttt tgtttcctcc acacgttgag gttgctgttc
      961 catgaaataa tgaatcctct ctgtgatcat cttcgacact tcaagcccct tatcgatcat
     1021 tctgtcactg caacccatct catgcagcag tttcgctttg taaatggttt ccacacgcga
     1081 aacaaacaca tccaccgctt catcctgaat gtcaacaacc aatgattcta tacacagtct
     1141 gatctcttct gttaaggcct ttgaggcttg tacttcatca ctgaggtaca tgtcgtggta
     1201 attcctgact tcaatgggtt cgcctatacg aatcaccaga tccgactgaa atttgtgtgg
     1261 atcggaatac gttaatccaa ccggtacaat gcggcaattg agacggaaat ccctttcctc
     1321 ttcagcccca agagccatgc gtgcagctcc ggtttttatc tcttttagct ttctgtcggt
     1381 tattgaaatt ccctcaggaa agatcagcac atttcctccc gactgcagca ggtcaaacac
     1441 ctttcgaaag gtgtccttgt ttttatgcat taatgaagga tcgtattgct tgcggtaaac
     1501 cggtatcata ttgaagcgtg gtagcaacca ttgggcaaat ttcgatttga aagcctccgc
     1561 tttggcaata aaattaactt ctcccggaat cacagtagca atgacgatgg gatccataaa
     1621 cgtgctggga tgattagcaa caataagtaa agggccgcta tttggaatgt tttcaatacc
     1681 caaaactttc ttatttctga agaatgcttt tactgcaatc cgaaacaaat atttgaggaa
     1741 tgaataaacc attgtgtcaa tttcgtaatt gaaattgaat tattcaaaaa gaataccaag
     1801 aatagcgaaa ttcaataagc caatacagga ctaagccatt tttccaccat tgagtaatcc
     1861 tcttgttttc gctttgccag atcttcaacc tgatccttct gtatttgacc caatccgaaa
     1921 tatttgcttt gcggatgtgc aaaataccag ccacttaccg aagctgcagg gtacatagcc
     1981 aggctttctg tcaaacttac acccgatcgt ctttctgcat ccaataattg aaaaatagtc
     2041 aacttttcag tatgatccgg acatgcagga taaccgggag ccggtctgat tcctctgtat
     2101 ttttcgctga tcagctcttc attagatagc ttttcacttg ctgcataggc ccaggcatct
     2161 ttgcgtacat attcatgaag atattctgca aaggcctctg caagacgatc tgcaagagct
     2221 ttaagcatta tgctgttgta atcatcatga tccttttcga acttctgaat ccacttctcc
     2281 attccaattc ctgcagttac cacaaacgca ccgatatgat cctgtaggcc ggtagacttt
     2341 ggtgcaacaa aatctgcaag gcacatattg tatgctccct gggttttttt ggtttgttgt
     2401 cgtaactgat gactaattgc gctaatctgt ttcctgcttt cgtcggaata aatctcaata
     2461 tcttcttttc cgtttgaatt tgcagggaag attccataaa ccgcgtttgc agaaatccac
     2521 ttttcggtaa caattcgatc aagaagtgca gttgcatcat tgaataactt cgtcgcttct
     2581 gcaccaacaa cttcatctgt aaggattttt ggaaaacgac ctgcaagttc ccatgtttgg
     2641 aaaaaaggtg tccagtcgat atatggaacc aatttttcaa gtggaaaatc attcaatacc
     2701 tgaacaccag gtttttttgg cgaatgaatg tcgtctgcat tccattcaac gggaaactta
     2761 ttggaacgtg cttcggtaag agaaataaaa ttcttttcac tacggtgacg cgcatgatgc
     2821 tctctcattt tgtcatactc actcctgata tcagctacaa aaacagattt attttcgccc
     2881 agtaattgtc caacaacagg aacagaccgt gaagcacaat attaaatact atgcacaacc
     2941 gcaacctacg gcaatcacat gccaaacaac tactagccaa aacagaaatc actccaccaa
     3001 aagaaagcaa ataaaaggca acaagatagc ctaccaacag acgacgacta atagcacaaa
     3061 ctatgttaa
//
LOCUS       DAOMYG010000393         3048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_11825_length_3048_cov_9.155697, whole genome shotgun sequence.
ACCESSION   DAOMYG010000393 DAOMYG010000000
VERSION     DAOMYG010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11825_length_3048_cov_9.155697"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PK610_11500"
     CDS             complement(<1..368)
                     /locus_tag="PK610_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRJ36608.1"
                     /translation="MSNALNVRTMAECLASGEVPEVLFWVGCSGSFDDRARKITRALV
                     KILNAANVKFAVLGTEETCTGDPAKRAGNEFLFQMQAMQNIQVLNAYEVKKIVTACPH
                     CFNTIKNEYPELGGNYEVMH"
     gene            complement(379..804)
                     /locus_tag="PK610_11505"
     CDS             complement(379..804)
                     /locus_tag="PK610_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HRJ36609.1"
                     /translation="MSDSISLIPIEENGQHLSPVLPSHIKNYLIDIDGTICDDIPNEE
                     PWRMVTAEVYPDALITLNKWFDEGHIITFFTSRTEDHREVTEEWLKKHGFKYQGLLMG
                     KPRGGNYHWIDNHMVRATRYEGKFTDLVVKEVTIQVFEK"
     gene            complement(804..1178)
                     /locus_tag="PK610_11510"
     CDS             complement(804..1178)
                     /locus_tag="PK610_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36610.1"
                     /translation="MKKNILLLLFLGFMMPFAASAQTIQKTTPAGIEFKYFKDTIIVG
                     YANEVRIVNAGSAAVQVVLNGCSVRPGDGSNVFNVLAPANLAGQEAEIIVSSSVNGVN
                     KVLVRKKVKFVASTPQLLNRMQ"
     gene            complement(1194..1568)
                     /locus_tag="PK610_11515"
     CDS             complement(1194..1568)
                     /locus_tag="PK610_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36611.1"
                     /translation="MKTFKVFFLLLFIGTSLSSGAQITKTVAPSLSFKYFKDTLVVDH
                     NMEVTVTNAANLTVTMAAQSCTVTRTDGGKTFIVKAPRALNGQRVDIVVTKTTSSGTV
                     EIIRRSVLVVAATPELISRMQN"
     gene            complement(1585..2907)
                     /locus_tag="PK610_11520"
     CDS             complement(1585..2907)
                     /locus_tag="PK610_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRJ36612.1"
                     /translation="MSIENIAFIALFVTAVTLFTLKIREIIANIRMGQAIDRSGNASE
                     RWKTMLLVAFGQKKMFSRPLPAVLHLAVYVGFVIINIELLEIIIDGATGSHRALSFLG
                     GFYNLLIASFEVLAFLVIVACVIFLARRNIVKVARFHYREMTSFPKSDANLILIAEVL
                     MMSAFLIMNASDLILQGRGVEHFTVAGAFPISQFLVPMMDGMSEGALVLLERACWWFH
                     IIGIFALLNYLPYSKHFHIVLAFPNTYFSNLSAKGRLTNPESVTREVKLMFDPTADPY
                     AAPADTNGAVQRFGAKDVPDLTWKQLLDAYTCTECGRCSSSCPANQTGKLLSPRKIMM
                     DTRDRMEEIRTNIKKHGQDHNDGKSLLGDYITAEEIWACTSCNACVQECPVNIDPLGI
                     IIDLRRYLVMEESKAPTELAGMFTNIENNGAPWQFSPADRINWANETN"
BASE COUNT          884 a          700 c          642 g          822 t
ORIGIN      
        1 tggtgcatca cctcgtaatt accacccagt tcagggtatt cgttttttat ggtgttaaag
       61 caatgcgggc aagcagtaac gatctttttc acctcatagg cattcaatac ctgaatgttc
      121 tgcatggcct gcatttggaa aagaaattca tttcctgcac gttttgccgg atcaccggta
      181 caggtttctt cagtacccaa aactgcgaat ttcacattgg cagcattcag aatttttacc
      241 aaagcacgtg taatttttcg cgcgcgatcg tcgaaagaac cggagcatcc tacccaaaac
      301 aaaacttccg gaacttctcc ggatgccagg cattctgcca ttgtgcgaac attcaatgca
      361 ttactcatcg gtgtgttttt atttttcgaa aacctgaatc gtgacttctt tcacaaccag
      421 atccgtaaat tttccttcgt atcgagtggc tctaaccatg tggttgtcga tccaatggta
      481 atttccacca cgtggctttc ccatcagcaa tccctggtac ttgaatccat gttttttcaa
      541 ccattcctcg gtaacctcgc ggtgatcttc ggtacgcgaa gtgaaaaagg tgatgatatg
      601 accttcatca aaccatttat tcagcgtgat caatgcatcc ggataaacct cagcagtgac
      661 cattctccac ggctcttcat ttggaatatc gtcgcagatc gtaccgtcga tatcgatcag
      721 gtaattttta atatgagaag ggagaacagg actaagatgt tgcccgttct cctcaattgg
      781 aatcaaagaa attgaatcac tcattattgc atcctgttaa gcagttgcgg agtactggcc
      841 acaaacttca cttttttgcg aaccaatact ttgttaacgc cattcactga agaagaaaca
      901 atgatctcag cttcttgtcc ggcaaggttt gcaggtgcaa gtacattaaa cacattgctt
      961 ccatcgccgg gacgaactga acaaccatta agtacaacct gtactgcagc agaaccggca
     1021 ttaacgatac gaacctcgtt ggcataacct acgataatgg tatctttaaa atatttgaat
     1081 tcaattccgg caggcgttgt tttctgaatg gtttgagcgg aggccgcaaa aggcatcata
     1141 aagcctaaaa acaaaaggag gaggatattc tttttcatag tctatagttt agtttaattc
     1201 tgcattctgg aaattaattc gggagttgct gcaactacca atacgctgcg tcggataatc
     1261 tctacagtac ccgaagatgt ggtttttgtt acaacgatat caactcgttg accatttaag
     1321 gctctcggcg ctttcacaat aaacgttttt cctccatcgg tacgggtaac agtgcacgat
     1381 tgtgcagcca ttgtaacagt gagattggca gcgttggtaa ccgttacttc catattatga
     1441 tctaccacca gcgtatcttt aaaatacttg aacgaaagcg agggtgcaac cgtcttggtt
     1501 atttgagcgc cggaacttaa gcttgttccg ataaaaagta acagaaaaaa aactttaaac
     1561 gttttcatgg cacttgattt aagattaatt ggtttcgtta gcccagttaa tgcgatcggc
     1621 aggcgagaat tgccatggtg ctccgttgtt ctcaatattc gtgaacatac cggcaagttc
     1681 cgtgggtgcc ttcgattctt ccataaccag gtacctgcgt aaatcaataa tgatgccaag
     1741 aggatcgata ttcaccggac attcctgtac acatgcatta caggaagtac aagcccagat
     1801 ttcctctgct gtgatatagt cgccaagcaa tgatttgcca tcgttatgat cctgtccgtg
     1861 cttcttaata ttcgtgcgaa tctcctccat tctgtcgcgg gtatccatca tgatcttacg
     1921 cggcgaaagc aatttgccgg tctggttggc aggacaagaa gacgaacaac gtccacactc
     1981 agtacatgta taggcatcca gcaattgttt ccaggtaaga tccggaacat ctttcgcacc
     2041 aaaacgctgt acggcaccgt tggtatcagc tggtgcagca taaggatcag cagtaggatc
     2101 gaacatcaat ttcacctcgc gggtaaccga ttcgggattg gtaagacggc ctttggcact
     2161 gaggttggaa aaataggtgt tcggaaaagc aagtacaatg tggaaatgct tggagtaagg
     2221 aaggtaattg agcaatgcaa aaataccgat gatgtgaaac caccaacagg cacgctccaa
     2281 taacaccaat gcaccttccg acattccgtc catcatcggc accagaaatt gagaaatggg
     2341 gaatgcaccg gcaaccgtaa aatgctcaac tcccctgccc tgcagtatga gatctgaagc
     2401 gttcatgata agaaatgcgc tcatcattaa aacctcagca atgagaataa ggtttgcatc
     2461 gctctttgga aaggaggtca tttcacggta atgaaaacgg gccaccttta caatgtttct
     2521 tcgggcaaga aaaattacac aggcaacaat caccaaaaag gcaagaacct caaatgaggc
     2581 gatgagtaaa ttgtaaaatc cacccaaaaa cgaaagcgca cggtgcgatc cggtagcacc
     2641 atcaataatg atttccaata actcgatatt gatgatcaca aaaccaacat acacggcaag
     2701 gtgaagtaca gcgggtaagg gtcgcgaaaa cattttcttc tgaccaaatg caaccagcaa
     2761 catggtcttc caccgttcgg atgcatttcc ggaacgatca atcgcctgac ccattcggat
     2821 attggctatg atctcacgaa tttttagggt aaaaagcgta accgcagtta caaaaagagc
     2881 aatgaatgca atgttctcga tcgacataat tattgctgca ggatttgttt caattttttt
     2941 tcttcacgtg catccagttt cagccctttt tcctttcggc caaaaactga aaaatgcaac
     3001 agcattacga cgagcaggtg ctgcagatcc acgtcatggc cgagtacg
//
LOCUS       DAOMYG010000394         3028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_11936_length_3028_cov_10.698621, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000394 DAOMYG010000000
VERSION     DAOMYG010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11936_length_3028_cov_10.698621"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="PK610_11525"
     CDS             complement(<1..1046)
                     /locus_tag="PK610_11525"
                     /inference="COORDINATES: protein motif:HMM:NF039639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HRJ36613.1"
                     /translation="MSANSHRLYLLAGFIALLPLFALSFFTFPCADDWCADIFFRQFH
                     VRSDAFNALYETYNGRFAANGVLLFSPLNFGQSGYSVWLFFQLLLFALVFFLVAKSLF
                     RYFGIERGSELISLLSLLTAFSALPQISEGLFWLTGSAAYLGGSIFLLLHIGSMIYYL
                     RKKNTSGLLFVFITAFLAQGWNEVQTVFMAAVYFIALIGFIIYKKKIPTWFIFSMIAI
                     SIGTLIMVLAPGNDVRINNYPDAGNTVNSLFMSFAQTLRFLALWLIHPSGIILAIIVM
                     YKGAQFGSTTRLPSLFILISLLLFAVFIAAFLPYLTTGILGQHRTLNWACVFCFLLLP
                     LIWFQVGFRLKNIIR"
     gene            complement(1047..1343)
                     /gene="trxA"
                     /locus_tag="PK610_11530"
     CDS             complement(1047..1343)
                     /gene="trxA"
                     /locus_tag="PK610_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRJ36614.1"
                     /translation="MSSFSELIKGDKPVLVDFFATWCGPCRMMSPILEDVTKKVGPKA
                     HVLKVDVDKNPQAAAHYGVQGVPTLILFKKGKIIWRQSGVIPANQLVNIIQQNS"
     gene            complement(1356..1808)
                     /locus_tag="PK610_11535"
     CDS             complement(1356..1808)
                     /locus_tag="PK610_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRJ36615.1"
                     /translation="MKKLIFPLATMVLLASCTSEKTHTETSTTSTTVTTTEGESTTTS
                     SSLSTVDAATFKSEMESTPGTLLDVRTPEEVAAGIIPGATVININDSDFETRIDQLDK
                     TKPVYVYCKAGGRSARAADMLKEKGFPVVVNLDGGIMAWTDKGFETAK"
     gene            complement(1860..>3028)
                     /locus_tag="PK610_11540"
     CDS             complement(1860..>3028)
                     /locus_tag="PK610_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRJ36616.1"
                     /translation="MTAVVNKLQRMLGLRIHEKYREKVLPKENDRPIVFITHMEHHSN
                     QTSWLETLADVRVIDANAEGLPDLNSLKNLLEEFKHRPMKIAAVTSCSNVTGVFTPYH
                     EIATVMHEADGLCFVDFACSAPYITIDMHPKEYPQGWLDAIYFSPHKFLGGPGTSGVL
                     VFNTKLYTNKIPDNPGGGTVDWTNPWGEHKYVDDIEAREDGGTPGFMQAIRTALCIRL
                     KEKMGVENIMKREEELLSIIWPGLSSIEGLHLLADNHPHRLGVLSFYVEGCHYNLAVK
                     LLNDKYGIQVRGGCSCAGTYGHYLLEVSYDKSKNITDKINQGILTEKPGWVRMSIHPT
                     MSDQEAKELVEAVKSLAAHHREWVKEYDYSSKTNEFTRKNFNDSREKVVLQWFS"
BASE COUNT          884 a          652 c          638 g          854 t
ORIGIN      
        1 cgaataatgt ttttcagcct gaatccaacc tgaaaccaaa tcagtggcaa aaggagaaaa
       61 caaaacacac atgcccaatt caaagttctg tgttgaccta atattcccgt tgttaaatag
      121 ggaagaaatg cagcaataaa aacggcaaaa agcagaagtg aaatcagaat gaacagcgag
      181 ggtaagcgag ttgttgaacc gaattgtgcg cctttataca tcacaattat ggcaaggata
      241 atgcctgatg ggtgaatcag ccacaaagcc agaaagcgca gtgtttgtgc gaaactcata
      301 aaaaggctgt tcactgtatt tcctgcgtcc ggataattat ttattcgcac atcatttccc
      361 ggagcaagta ccataatcaa tgtgcctatg ctgatggcaa tcattgaaaa gataaaccat
      421 gttggaattt ttttcttgta gatgataaaa ccaattaagg cgataaaata aactgctgcc
      481 ataaacactg tttgaacttc attccatccc tgtgctaaaa acgctgttat gaaaacaaaa
      541 agtaatcctg atgtgttttt ttttcggagg taataaatca tacttccaat gtgcaacagc
      601 aaaaaaatgg atccgccaag ataggctgcg ctgccggtca gccagaatag accctcagaa
      661 atttgcggta atgcagaaaa ggcggtaagc aatgagagca ggctgattag ttcactacct
      721 ctttcaattc caaaataccg aaagaggctt tttgcgacaa gaaaaaaaac caatgcaaag
      781 agaaggagtt gaaagaacag ccaaactgaa tatccggatt gcccaaaatt taatggagaa
      841 aaaagcaata ccccgttagc tgcaaatctt ccattgtaag tttcatacaa ggcattaaat
      901 gcatcggatc gcacatgaaa ttgcctaaaa aaaatatctg cgcaccagtc atcagcacag
      961 ggaaaggtaa aaaaagataa tgcgaaaaga ggaagtaatg caatgaatcc tgcaagcaga
     1021 taaagtcgat gcgaatttgc gctcaatcag ctgttttgtt gaatgatatt tacaagctga
     1081 ttggcaggaa ttacccccga ttgtctccag ataatttttc cttttttaaa caggattaat
     1141 gtaggtacgc cctgcactcc gtaatgtgct gctgcctgag gattcttgtc cacatcaact
     1201 tttagcacat gggcttttgg accaactttt ttagtgacat cctcaagaat tggcgacatc
     1261 atcctacacg gaccacacca ggtggcgaaa aaatcgacaa gcaccggctt atctcctttg
     1321 atgagttctg agaaactaga catagtgcaa ttcttttatt tcgcagtttc aaatccttta
     1381 tctgtccatg ccatgattcc accatcgagg ttaacaacaa caggaaatcc tttttctttc
     1441 agcatgtcag cagcgcgagc tgaacggcca ccggctttac agtaaacata aacaggtttg
     1501 gttttgtcca gctggtcaat gcgtgtttcg aaatcgctat cattaatgtt gatcacagta
     1561 gcaccaggaa tgattcctgc agcaacttct tcaggtgttc ttacatctag tagagtaccc
     1621 ggtgtacttt ccatttctga tttgaatgtg gctgcatcta ctgtactgag cgaactgctt
     1681 gttgttgtcg attcgccttc ggtagtagta actgtagtag aagtagtgct cgtttctgta
     1741 tgtgtctttt cagaagtgca tgatgcaaga agcaccattg ttgccaaggg gaagatcagt
     1801 tttttcatac tgcaaagata cactacctga acaatacggt cagtgatgcc tgtcacaatt
     1861 cacgaaaacc attgcaggac taccttttca cgggaatcat taaaattctt tcgtgtgaat
     1921 tcattggttt ttgaagagta atcatactcc tttacccatt cgcgatgatg agctgcgagt
     1981 gattttactg cttccaccaa ttcctttgct tcctgatcgc tcatggtagg gtgaatcgac
     2041 atacgcaccc agcccggttt ttcggtgagg atgccctggt tgattttatc cgtgatgttt
     2101 ttcgatttat cgtacgacac ctccagcaga taatgtccgt aagttccagc gcatgaacaa
     2161 ccacctctta cctgaatgcc gtatttgtcg ttgagaagtt tcacggcaag attataatga
     2221 cagccttcaa cgtaaaacga aagtacgcct aaacgatgcg ggtgattgtc ggccagaaga
     2281 tgcaatcctt caatggaact taatcccggc cagatgatgg agagaagttc ttcttcgcgt
     2341 ttcatgatat tttcgacacc cattttttcc ttgaggcgaa tgcacaatgc agtgcgaatg
     2401 gcttgcataa aaccgggcgt tccaccgtct tcgcgtgctt caatatcgtc cacgtatttg
     2461 tgctctcccc atgggttggt ccagtcgact gttccgcctc cgggattgtc gggaatttta
     2521 ttggtgtaca gtttggtgtt gaatactaaa acaccgcttg tacccggccc gccaagaaat
     2581 ttgtgcggtg agaaatagat tgcatcgagc caaccttgcg gatattcttt tggatgcata
     2641 tcgatggtaa tataaggcgc actacaggca aaatcgacaa agcacaaccc gtctgcttcg
     2701 tgcataactg tagcaatttc atggtaggga gtaaatacac cagttacgtt actgcaggat
     2761 gttaccgccg caattttcat cggacgatgt ttaaactctt ccaataaatt tttcagcgaa
     2821 tttaaatccg gtaatccttc tgcattggca tcgatcactc taacatcggc taatgtttcc
     2881 agccaggacg tttggttgga atggtgttcc atatgggtga tgaaaacgat cggacgatcg
     2941 ttctcctttg gtaaaacttt ttcgcggtac ttttcatgaa tacgcaatcc gagcatgcgt
     3001 tgaagtttat tcaccaccgc tgtcattc
//
LOCUS       DAOMYG010000395         3028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_11938_length_3028_cov_7.507904, whole genome shotgun sequence.
ACCESSION   DAOMYG010000395 DAOMYG010000000
VERSION     DAOMYG010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11938_length_3028_cov_7.507904"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PK610_11545"
     CDS             complement(<1..373)
                     /locus_tag="PK610_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36617.1"
                     /translation="MKQTFLISAFALFCLFSCAEQSQTDSTTKQDSDSTEKSTETAER
                     GVSGIDTIQEQNDADENEVQHYQKEMDLFWIRDMEENANKKVGFVSLSDVYSWSDDPA
                     VQPISDLESREKNSAHNFKLNS"
     gene            complement(628..1710)
                     /locus_tag="PK610_11550"
     CDS             complement(628..1710)
                     /locus_tag="PK610_11550"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRJ36618.1"
                     /translation="MKNKMIAIMVVTLLFYSCSETGKLHHGKESTTNKPSGVIADTVA
                     QPGNNIMVIYQDKQNNYWFGSWEDGLYKYDGKLVLHYTTKQGLPSNRVEEIKEDKLGN
                     LYFNTSGGIIKYDGDHFIQLQESIGDLEMWSLSSSDLWFKNFTNAQSVFRYDGIALHQ
                     LKIPKVALGEEWIEKSPNSLNPYAIYCTYKDSKGHVWFGTAAIGVFRYDGTKFDWITE
                     NDVTELHYGPSNGVRSIAEDKDGCFWFNSNYRYKITDNNAISGSPFYERQKSIGNLDG
                     KPDSNINEYLSIVRDNANNMWIATYLDGVWKYDGATVRHYAVQENGKNIKLFYLYKDN
                     KGTIWLGTHENGVWSLKGDAFVRLVL"
     gene            complement(1730..2815)
                     /locus_tag="PK610_11555"
     CDS             complement(1730..2815)
                     /locus_tag="PK610_11555"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRJ36619.1"
                     /translation="MIKSMSLLAMIALVVLQSACSGNRVAKSSQEAIEINLSDAKDTL
                     KFTSGIRSIFQDSKGNYWFGSHQEGVCKYDGKTFSYYTTENGLCGNQVITIGEDETGT
                     LWFGTGSGLCSYDGTVFTTATGSSDFPKIRLNNSENSSFVSTATDLWFAGENSNEIIR
                     IDKGRIHKIHYPLRIPAKWNEGDYGITGFSKSNKGGLWIAHYSGVAYFDGNAIQFLND
                     STMHFDGESKYMHVRSILEDSKGRLWIGNNGIGVQLKEKDSIIHFSEKFNLFKGEAIR
                     VKSPAGTLMHVFAIHEDSKGNIWFGDRDTGIWRYDGKEMKNFYLDFNLNTQHIWDIYE
                     DRSGNLLFASADRGVFRYNGNGFDRVF"
BASE COUNT          828 a          669 c          569 g          962 t
ORIGIN      
        1 aagaattgag tttaaaattg tgagcactgt ttttttcgcg gctttcaaga tcagaaatgg
       61 gttgtacagc gggatcatcg ctccaggagt aaacatccga caaagaaaca aaacccactt
      121 ttttatttgc attttcttcc atatcgcgga tccaaaacag atccatttcc ttttggtagt
      181 gttgaacctc gttttcatca gcatcattct gctcctgaat ggtatcaata ccgctcacac
      241 ctcgctcagc agtttcagtg ctcttttccg tgctgtcgga atcctgtttt gtagtggaat
      301 ctgtttgcga ttgttcagcg caggaaaaca aacagaacaa agcaaaagcg gatatgagga
      361 aggtttgttt cattgggggg gggctaagtg atagatagat ataataacaa gatcaaaaat
      421 tagtacaagt acatttattg tttgcaatga acctttgaaa ggttgataat aatataactg
      481 cttaacggta aggtccgttt cattgggctt tccatacgta aaataccaaa ttcctaaaac
      541 ttatgaaaat tgaagacttt taggaatttt ggaaaagtcg ttacgttccg tttattgagc
      601 cttctgcacc cctaaagtct ttggcaatca aagcaccaag cgcacaaacg catccccctt
      661 caaactccat actccgtttt cgtgggtgcc cagccaaatg gttcctttgt tgtctttgta
      721 gaggtaaaat aactttatgt tcttcccgtt ttcctgtacc gcataatgac gaactgttgc
      781 gccatcgtat ttccaaacgc catccaggta ggtggctatc cacatattgt tcgcgttgtc
      841 tcttacaatg gataaatact cattgatgtt gctatcgggt ttgccgtcta aattgccaat
      901 gctcttttga cgctcgtaaa agggagatcc cgagattgca ttattgtctg tgattttata
      961 acggtaattg gaattgaacc aaaaacatcc gtctttgtct tcagcaatag agcgaacacc
     1021 atttgagggt ccgtaatgaa gttcggtcac atcattttct gttatccaat caaacttggt
     1081 accgtcgtaa cgaaacacgc caattgctgc tgtgccgaac catacatggc ccttgctgtc
     1141 tttgtaggta cagtaaatcg catagggatt taatgagttt ggactttttt caatccattc
     1201 ttcgcctaac gcaactttgg gaatttttaa ttggtgcaaa gcaatgccgt cgtacctgaa
     1261 gacagattgt gcattcgtga aatttttaaa ccagagatca ctggacgata aactccacat
     1321 ttcaagatct ccgatgcttt cctgcaattg aataaagtga tcaccatcgt atttaattat
     1381 tccaccactg gtattgaaat aaagattccc caatttatct tctttgattt cctctactct
     1441 attcgagggc aaaccttgtt tggtggtgta atgaaggaca agctttccgt cgtatttata
     1501 cagaccgtct tcccaactac cgaaccaata attgttctgc ttgtcctgat aaataaccat
     1561 gatgttattg cccggctgag ccactgtatc agcaattaca cctgaaggct tgttggttgt
     1621 tgactccttt ccatggtgca gcttacctgt ttctgagcag gaataaaaca gcagggtaac
     1681 aaccattatc gctatcattt tatttttcat ttttaatctg ttgcttgtat cagaataccc
     1741 tgtcgaaacc atttccatta tacctaaaaa cccctctatc tgcggaggca aaaagtaaat
     1801 tgccacttct gtcttcgtag atatcccaaa tatgttgtgt attcaggttg aaatccagat
     1861 agaagttttt catttctttg ccgtcgtatc tccagattcc ggtatcgcgg tctccgaacc
     1921 aaatgttccc tttggagtct tcgtgtatgg caaacacatg catcaaggtt cctgcgggtg
     1981 actttacgcg aatagcttcg cccttaaaca aattgaattt ttcagaaaaa tgaatgatgg
     2041 aatccttttc cttcaattgc acgccaatac cgttgttgcc aatccataaa cgacctttac
     2101 tgtcttccaa aatacttctt acgtgcatgt atttcgattc accatcaaaa tgcattgtgc
     2161 tatcgttcaa aaactgaatg gcatttccat cgaaataggc aacacccgaa taatgagcaa
     2221 tccacaagcc ccccttattg ctttttgaaa agcccgtaat accataatca ccttcattcc
     2281 atttggcagg gatcctaagg ggataatgaa ttttgtgaat tcttccttta tcaattcgta
     2341 tgatttcatt gctgttttcg ccggcaaacc agagatcggt ggcggtactg acaaaggaag
     2401 aattttcgct attgtttaaa cgaattttcg ggaaatccga actccccgtt gcagttgtaa
     2461 atactgtgcc atcgtaacta cacaatccgc taccggttcc aaaccaaagg gtgcctgttt
     2521 catcttcgcc gatcgtaatg acctgattcc cgcataaacc gttttccgtt gtgtaatagg
     2581 aaaaagtctt tccgtcgtat ttacatacac cttcctgatg gctgccgaac caatagttac
     2641 ccttgctatc ctgaaaaatg gaacgaattc cgctcgtaaa tttcagtgta tcctttgcgt
     2701 cagaaagatt aatttctata gcctcctgac tgctctttgc cactctgtta ccggaacaag
     2761 cggattgcaa cacaactaag gcaatcatag ctaaaagtga catcgatttt atcatctttt
     2821 cgaaataggg ttatccatta caaatcagct tcgcccctac tctgtactcc ggtctttgtc
     2881 cggtagtctg cactatgact tattgacttg ccggataaat gtattccatt ttagttctgc
     2941 tcaggtaagg gcaggtagat tttgggaaat tttaacagag agatctagat ttggaagctt
     3001 acagcataat ttgctgctta cttagatc
//
LOCUS       DAOMYG010000396         3018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_11997_length_3018_cov_7.858589, whole genome shotgun sequence.
ACCESSION   DAOMYG010000396 DAOMYG010000000
VERSION     DAOMYG010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11997_length_3018_cov_7.858589"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1424
                     /gene="mtaB"
                     /locus_tag="PK610_11560"
     CDS             96..1424
                     /gene="mtaB"
                     /locus_tag="PK610_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRJ36620.1"
                     /translation="MNQYRKVSFYTLGCKLNFSETSTIARQFVEAGYARVDFFDRPDY
                     YVINTCSVTDNADKKCRQLVRRALKVNPDARIVIIGCYAQLKPQEIAQIDGVDLVLGA
                     NEKFNVIEHLENLADNQKGQIEASEIKQVKEFIPSFSYGDRTRSFLKVQDGCDYFCSF
                     CTIPLARGFSRSHTVQETIKVAREVANTSVKEVVLTGVNIGDFGSGTNENFLDLIREL
                     DEVEGIDRYRISSIEPNLLSDAIIDFVSVSKRFAPHFHVPLQSGSDKILKLMRRRYER
                     SLYADRVARIKQRMPDACIGVDVIVGFPDETDEDFLDTYEFINSLDISYLHVFTYSER
                     DNTTAVRMKEVVPADVRNKRSKMLHILSEKKKRAFYATQTGKVVRVLFEAEEEEGMMF
                     GFSENYVKVKLPFDPTLVNRFADVRLSAIDRDGVMKTEVLSVVPEIPIAI"
     gene            1424..2578
                     /locus_tag="PK610_11565"
     CDS             1424..2578
                     /locus_tag="PK610_11565"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRJ36621.1"
                     /translation="MYPTLYHFFYDLTGFEIPFFRAVNSFGFFVAISFVVASWVYSRE
                     LKRLEESGFIPVGKIVRTYGLPVPLWDVAGNALLGFFLGFKIVHGFFYPEIFSNFPAF
                     LFSLEGNIFGGILGGLAMGAWRYYEGKKLERPTPEVREEAFHAYEHLGKLVLLAVVWG
                     FIGAKLFAWLEDPQPLGEFLSDPFRGLTMYGGLICATIAMTLYMRKHKLPVWRFYDAG
                     APAVMLAYGVGRIGCHVSGDGDWGIVNTAPKPGWMSFLPDWLWSYNYPNNVMKMGTPM
                     SDCIYNDDYCYVLPQTVFPTPFYETLMCLLFFGVLWLLRKRILVPGVLFMVYLMLNGV
                     ERFLIEGIRVNDKYMAGGLAFTQAQLISFVFIIAGAAGIFFLRKRYRQAT"
     gene            2575..>3018
                     /locus_tag="PK610_11570"
     CDS             2575..>3018
                     /locus_tag="PK610_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRJ36622.1"
                     /translation="MSLVFIVKSSIRRKTAFYQLAEEFRSYGLFERIEIRETERHQHA
                     VTLAFQAAREGFQYVIAVGGDGTLNEVINGVMAHKETLSVVGFLPFGSANDFSRSLPC
                     FYSAAEIAEAIRVGKFFSCDLGFLNSVDLESKTIKNWFVNVFDAGI"
BASE COUNT          721 a          593 c          741 g          963 t
ORIGIN      
        1 gttgaataat tgcatttctg aaaatgaaga agaaaggggg ctacggtccc ctttttttgt
       61 tgggttttga agaaagtgga ctacttttgc agcttatgaa tcaataccgg aaagtcagtt
      121 tttataccct cggctgtaag ctcaactttt cagagacatc aaccattgcc cgtcagtttg
      181 tcgaggccgg ttatgcccgt gtcgatttct ttgatcgtcc ggattattac gtcatcaata
      241 cctgctcagt aaccgataat gccgataaaa aatgccgcca attggtccgc agagccctga
      301 aggtcaatcc tgatgcgcgt attgtgatta ttggctgtta tgcccagctg aagcctcagg
      361 agattgcaca gatcgatgga gttgacctgg ttttgggggc caacgaaaaa ttcaatgtca
      421 ttgaacacct cgaaaatctt gccgacaatc aaaagggtca aatcgaagca tccgagatca
      481 aacaggtaaa agaatttatt ccctccttca gttacggcga tcgcactcgc agctttttga
      541 aggttcagga tggttgcgat tatttctgtt ccttctgcac cattccgctg gcaagaggtt
      601 tttcgcgaag tcatacggtg caagaaacca tcaaggttgc tcgcgaagtg gcgaatactt
      661 ctgtgaagga agtggtgttg accggtgtaa acattggcga ttttggttcc ggtaccaatg
      721 aaaattttct tgatctgatt cgtgaattgg atgaagtgga aggaattgat cgctacagga
      781 tttcttcgat tgaacccaat ttactaagcg atgcgatcat cgattttgta tcagtatcca
      841 agcgatttgc tcctcatttt catgttcctc tccaatccgg ttccgacaaa attctaaaac
      901 tgatgcgccg caggtacgag cgttcgctct atgccgatcg tgttgcacgc atcaaacaac
      961 gtatgccaga tgcctgtatc ggtgtggatg tgattgttgg ttttcccgat gaaacggatg
     1021 aggattttct ggatacttac gaattcatta atagtctcga catttcttac cttcatgtgt
     1081 ttacctacag cgaacgcgat aataccactg ctgtgcgcat gaaggaggtt gttccggccg
     1141 acgttcgcaa caagcgcagt aaaatgctgc atattctctc cgaaaagaaa aaacgcgctt
     1201 tttacgcgac acaaaccggc aaagtggttc gtgttttatt tgaagctgag gaagaagaag
     1261 ggatgatgtt tggcttcagc gagaattatg taaaagttaa attacctttc gatccaacac
     1321 ttgtaaaccg ttttgcggat gtccgcctga gcgcaattga tcgcgatggt gtgatgaaaa
     1381 cagaagtatt aagtgtggtt cctgaaatcc caattgcaat ctgatgtatc ctacgctcta
     1441 tcattttttt tacgacctca ccggatttga aattcctttt ttcagagccg taaacagttt
     1501 tggttttttt gttgctatat cattcgttgt ggcatcctgg gtctattccc gcgagttaaa
     1561 acgattggaa gagtccggtt ttattcctgt tggaaaaatt gtgcggactt acggattgcc
     1621 tgtccctctt tgggatgttg caggaaatgc cttgttgggt tttttccttg gatttaaaat
     1681 cgtacacggc tttttttatc ccgagatctt ttccaatttc cctgcttttc tgttttcgtt
     1741 ggagggaaat atttttggag gaattctcgg cggactggca atgggtgcat ggcggtatta
     1801 cgaaggcaaa aaactggaac gtccaactcc cgaagtgcgc gaagaggctt ttcatgccta
     1861 cgaacatttg ggtaaacttg ttttgcttgc tgttgtttgg ggcttcatcg gagcgaaatt
     1921 gtttgcatgg ctggaagatc cgcaacccct gggtgaattt ttaagcgatc cttttcgtgg
     1981 tcttaccatg tatggcggat tgatctgcgc caccattgca atgactttat acatgcgtaa
     2041 gcacaaactc ccggtttggc gtttttatga tgcgggtgct cctgctgtaa tgctggcgta
     2101 tggtgttggt cgtattggtt gccatgttag tggtgatggc gattggggaa ttgtaaatac
     2161 tgcaccaaaa cccggttgga tgagtttttt gcccgattgg ttatggtcgt acaactatcc
     2221 caacaacgtg atgaaaatgg ggacaccaat gagcgattgc atttacaacg atgattattg
     2281 ttacgtgctc ccgcaaacgg tttttccaac tcctttttat gaaacgctga tgtgtctctt
     2341 gttttttggg gtgctgtggt tgcttcgcaa aagaattctt gttccgggtg ttttgtttat
     2401 ggtgtatttg atgttgaacg gagttgaacg ttttctgatt gaagggattc gcgtaaacga
     2461 caaatacatg gcgggtggac tcgcatttac gcaggctcaa ttgatttctt ttgtatttat
     2521 tatagcaggt gcagcaggca ttttcttttt acgtaagcga taccggcaag ctacatgagt
     2581 ctggttttta tagttaaaag ttcaatccgg cgaaaaacgg ctttctatca gcttgctgag
     2641 gagtttcggt cgtacggttt gtttgaacgg attgagattc gtgaaacaga acgccatcag
     2701 catgcagtta cccttgcatt tcaggcagcg cgtgagggat ttcagtatgt gatcgctgtt
     2761 ggaggtgatg gaacgctgaa tgaagtgatc aacggtgtga tggctcataa agagacctta
     2821 tctgttgttg gttttttgcc ctttgggagt gccaacgatt tttcccgttc cttgccttgc
     2881 ttttattctg ctgctgaaat tgccgaagca attcgtgtcg ggaagttttt ttcctgcgat
     2941 ctgggttttt taaacagtgt tgacctcgaa agcaaaacca ttaagaattg gtttgtgaat
     3001 gtttttgatg caggtatc
//
LOCUS       DAOMYG010000397         2995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_12154_length_2995_cov_10.004762, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000397 DAOMYG010000000
VERSION     DAOMYG010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12154_length_2995_cov_10.004762"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PK610_11575"
     CDS             complement(<1..587)
                     /locus_tag="PK610_11575"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRJ36623.1"
                     /translation="MRRIITLSFLVFMLPFQLLTAQTSYLFSQVDTVVVRFNLDTFNL
                     AWAGGINYGQFSNIDLNGDGIKDLFVFDRTGNKPLCFVHQGSINSIEYRHEPEYELAF
                     PAMQAWALLKDYNGDGKEDLWTYTPGGIKLYTNTTPVGGPLSFQLFIPLIRSNQLGNM
                     INLYVSSVDLPGIVDIDGDGDLDVLTFGVIGTSLE"
     gene            797..>2995
                     /gene="secD"
                     /locus_tag="PK610_11580"
     CDS             797..>2995
                     /gene="secD"
                     /locus_tag="PK610_11580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRJ36624.1"
                     /translation="MQNKSAIWTFTILLTLACLFQISYSWVTNSVEKEAEQVALDQLD
                     SIIQTKQTVVVYGSDQYEMAKEIDVENLKNRLTEDYLRKVAHEPAYPLLGESYQQCKK
                     QELSLGLDLKGGMAVTLEVSIPDLVVNLAGKSKNPTFRKAYESAYGKWVKNQSEDFID
                     LYAAEYLANNPDGKLVTPFSAANKDKFPSKMSNDELIAKLHAERDIAMANIEEIMNNR
                     INKFGVSNATLQRLGNSGRIQIELPGVKDKIRVRNLLQSTANLEFWETYNNHEIAPFL
                     ETANSIVSKALFPEIYTEGAAIEKPADSTVTATTTEPSTAVNTDSTKTTDSTLATTAN
                     NDSSLAESTKPDSLMNDLERKKKYPIYSYLNLAINKQSDNNFTWEQGSRIGFSPIADT
                     ARLNGLLAHPGLKGVFPDDLRLLWGSKPDFYNGEESQYISLYCIKTGRKGVPELDGSA
                     VSEARYDFDQFSGRVQVLMQMNAKGAETWANLTEKNKGKAIAIVMDDYVYSAPTVQDK
                     ITGGRSSISGNFTVDEANDLANVLKSGTLPARAQIVDSVEVGPSLGQSNITSGLNSFL
                     IALMIVLVYMYIYYKKAGLIANIALVANLFFLIGALASIQASLTLPGIAGIVLTIGMA
                     VDANVLIFERIREELRGGKSVKVAVNDGFKKALSSIIDGNLTTLLTAIVLAAFGSGPI
                     LGFATTLIIGIFTSLFSSIFISRLIIHELLERKKEISFSSKMTENILTNTK"
BASE COUNT          903 a          661 c          604 g          827 t
ORIGIN      
        1 tattcaagtg aagtaccaat cacgccaaac gtgagtacat ccaaatcacc atcgccatca
       61 atatctacta ttccgggtag atcaacagaa ctcacataga ggttgatcat attccctagc
      121 tgattggaac ggatcagcgg aataaataac tgaaaactta aaggacctcc aaccggagta
      181 gtattcgtgt acagttttat tccccctgga gtgtaggtcc acaaatcttc tttcccatct
      241 ccgttatagt ccttcaataa agcccaagcc tgcattgccg gaaaagcgag ttcatattca
      301 ggttcgtgcc tgtattcaat tgaatttatt gaaccttgat gtacaaaaca aagcggctta
      361 tttccggtac ggtcaaaaac aaaaaggtct ttgatcccat caccgttcag atcaatgttt
      421 gaaaactgac cataattgat ccctccggcc cacgcaagat taaaagtatc aagattgaat
      481 cgtacaacaa ccgtatccac ctgactgaac aaatagcttg tctgtgctgt cagcaactga
      541 aacggcagca taaaaacaag gaaagagagg gtgattattc ttcgcataca attgttattc
      601 atcataaacg tggaaacacc cgtaaagatg ccttcagttg cggcaaaaat acataaatcc
      661 ttaatggacg aactagctaa gcatacaaac ggttgaaata caatatctta cccattatta
      721 acactttgat agtgtaaata aggacctaaa ttctatattt gcaccccaat ttaattaacc
      781 gaaaaactgt ttttcaatgc agaacaagag tgcgatctgg acattcacga tccttcttac
      841 cttagcgtgt cttttccaga tatcctattc ctgggtgacc aatagcgtag agaaagaagc
      901 ggagcaagta gctctggatc aacttgattc gatcatacag accaaacaaa ccgtagtagt
      961 ttacgggtct gatcagtatg agatggccaa ggagatcgat gtagagaatt taaaaaaccg
     1021 tcttacagaa gactacctgc gaaaagtggc tcacgagccg gcttaccctt tgctgggaga
     1081 atcttatcag caatgtaaga agcaggaatt aagtcttggc cttgacctca agggcgggat
     1141 ggcggtaacg cttgaagttt ctattcctga cctggtggta aatcttgccg gtaaaagcaa
     1201 gaatcctaca ttccgtaagg cgtatgagag tgcctatggg aaatgggtga aaaatcaatc
     1261 ggaagacttc attgatttgt atgctgccga atatctggct aacaatccgg atggtaaatt
     1321 agtaactccg ttttctgcag ctaataagga taagttcccc tccaaaatga gcaacgacga
     1381 gcttatcgcc aagttgcacg cagaaagaga tattgcaatg gccaatatcg aggagatcat
     1441 gaacaaccgc atcaacaagt ttggtgttag caatgcaaca cttcaacgcc ttggcaacag
     1501 cggacgtatc cagattgaac ttcccggtgt aaaagacaag atccgtgttc gtaatctttt
     1561 gcagagtaca gccaaccttg agttctggga gacctataac aatcacgaga ttgccccatt
     1621 cctggaaact gccaactcca ttgtttcaaa ggctttgttc cctgaaatct ataccgaagg
     1681 tgctgcaatc gaaaagcctg ctgattctac ggtaactgca acaactacag aaccatcaac
     1741 tgccgttaat actgattcta ccaagactac agatagcact ttggcaacaa cggctaataa
     1801 cgattcatca ttggctgaat caaccaagcc tgacagtctg atgaatgatc ttgagcgtaa
     1861 gaagaagtac ccaatctatt cttacctgaa tcttgcgatc aacaaacagt cagacaataa
     1921 ttttacatgg gaacaaggtt cgcgtattgg attctctccc attgcagaca ctgcccggtt
     1981 aaacggtctg ttggctcacc ccggtttgaa aggtgtattt cctgatgacc tgcgattgct
     2041 ttggggttct aaacctgact tttataatgg tgaagaatcg caatacatca gtttgtattg
     2101 tattaagaca ggacgtaaag gtgttcctga actagacggt tcagcagtat cagaagcccg
     2161 ttacgatttc gatcagtttt caggtcgtgt tcaggtattg atgcaaatga atgcaaaagg
     2221 tgctgaaacc tgggctaatc taactgagaa aaacaaaggc aaagcgattg ccattgtaat
     2281 ggacgactac gtttattctg caccaactgt acaggataaa attaccggcg gacgttcatc
     2341 catctccggt aactttactg tagacgaagc aaatgacctt gccaacgttc ttaagtcagg
     2401 tacacttcct gcacgtgcac aaattgttga ctccgttgaa gttggtccat cacttggaca
     2461 atccaatatc acttcaggat tgaactcgtt tctgattgca ctcatgattg tacttgttta
     2521 catgtacatc tactacaaga aagccggttt gattgcaaac atcgcattgg ttgcaaacct
     2581 tttcttcctt atcggtgcac ttgcatccat tcaggcatca ttaacattac ccggtattgc
     2641 cggtatcgtt ctaacgattg gtatggcggt tgatgccaac gtacttatct tcgaacgtat
     2701 tcgtgaagaa ttacgcggag gaaaatctgt gaaggttgct gtaaacgacg gtttcaaaaa
     2761 agcattgtca tccattatcg atggtaacct tacgaccttg ttaacggcga ttgtacttgc
     2821 tgcttttggt tcaggtccga tccttggatt tgctactacc cttatcatcg gtattttcac
     2881 ttcacttttc tcttccatct ttatttcgcg tttgatcatt catgaattgc ttgagcgtaa
     2941 aaaagagatt tcgttctcct ccaaaatgac tgaaaacatc cttaccaata ccaag
//
LOCUS       DAOMYG010000398         2994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_12160_length_2994_cov_10.412385, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000398 DAOMYG010000000
VERSION     DAOMYG010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12160_length_2994_cov_10.412385"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="PK610_11585"
     CDS             complement(<1..151)
                     /locus_tag="PK610_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HRJ36625.1"
                     /translation="MKRIRNIHPGEVLLEEFLLPMAITAYKLSKDISIPQTRISEILK
                     GNRRIT"
     gene            complement(153..434)
                     /locus_tag="PK610_11590"
     CDS             complement(153..434)
                     /locus_tag="PK610_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019670432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRJ36626.1"
                     /translation="MIVSFGTRETEKIWEGQAVRHIPNEIQAVARRKLRMINNSISIA
                     DLKIPPANRLEKLKGDLSQFYSIRINKQWRIIFKWNNGNASNVEIIDYH"
     gene            complement(538..1512)
                     /locus_tag="PK610_11595"
     CDS             complement(538..1512)
                     /locus_tag="PK610_11595"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRJ36627.1"
                     /translation="MKIVLKVLKIAVPLALGIYLAWYSFDSIQDKDKVAQVMGRANYF
                     FIALSVIFSWCSHFVRALRWKYLLEPMGYQPRVWNSYHATMIGYFMNLLLPRAGEVSR
                     AGMMTRYEKIPFEKAFGTILAERVVDVFMLGIVSLITIALQYDKFIELKDTFSAMGSG
                     DQKSSSWMFIALVVLSALLLAGVVLYFIHPKVRTKVNALVSGLFDGLLAILRLKKRWE
                     FLGLTFLIWFLYILLYLVCFYSIAETSQLELKALLLGFLGGSLGIILVQGGVGVYPVL
                     VASALVMYGADYDVVIALGWVTWAAQTLLLVVAGAVSFYLMPRMNEEG"
     gene            complement(1509..1862)
                     /locus_tag="PK610_11600"
     CDS             complement(1509..1862)
                     /locus_tag="PK610_11600"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016479237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRJ36628.1"
                     /translation="MEIQVLKSKIHRCRVTEANLDYIGSITIDENLMDAANLIENEQV
                     HVLNYRNGERIITYVIKGERGSGVICLNGPAALKFTVGDDVIVISYAGMDFEKAKSFR
                     PHVIFPDAATNSLKA"
     gene            complement(2159..>2994)
                     /locus_tag="PK610_11605"
     CDS             complement(2159..>2994)
                     /locus_tag="PK610_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRJ36629.1"
                     /translation="ETYDTLGNQKKVNYALNPALSKEEFKKQLLTGLTPPPGYFPQNV
                     VLNIKGYDSFDKVMDRGMRALNPVEFEVAANETGALIIDTRRAEEFHKGFIPNAVNIG
                     IDGSFAVWVGTLVPDVKQPLLLIIEEGREEEVITRLSRVGYDQPLGYLKGGIAEWIKA
                     AKETDTITSVSADEFAVAYKKGNANILDVRKASEYFSEHIVEAENAPLDYINDSMLKV
                     DKNKSYYVHCAGGYRSMTFVSILQARGYRNLINVAGGFSAIKATGKFRISDYVCPTTM
                     L"
BASE COUNT          835 a          709 c          662 g          788 t
ORIGIN      
        1 cagtgatcct tcgatttcct ttcagaatct ctgaaatgcg ggtctgcgga atggagatat
       61 ccttggataa tttataggcc gtaatggcca ttggaagtaa aaactcctcc aacaaaactt
      121 ctccggggtg aatatttcta attcgtttca tgttaatggt aatctataat ttcaacattc
      181 gaagcatttc cattgttcca tttgaaaatg atccgccact gcttattgat tcgtattgaa
      241 taaaactgac tcaggtcgcc tttaagcttt tccagccgat tcgcgggtgg aattttcaaa
      301 tcagcaatac ttatcgagtt gttgatcatt ctcaatttgc gtcgcgctac ggcctgtatt
      361 tcattcggaa tatgacgaac cgcctgtccc tcccaaattt tttcagtttc ccgggttccg
      421 aatgaaacaa tcatagtaac gcatcacgat agtaacgcca aagataagta aacaatgaat
      481 acaatcaaag gaaagttagt ggtcctttcg tgctaagtgt ctttgtggtg aaaaaaatca
      541 cccctcctca ttcatccgcg gcatcaggta aaacgacacc gctccggcaa ccaccagcaa
      601 caaggtctgc gctgcccagg ttacccagcc caaggcaatc accacatcgt aatcggcgcc
      661 gtacatcacc aatgcagaag ctaccagtac cggatacaca cccacgccac cctgcaccag
      721 aataattcct aaacttcctc ccaaaaatcc aaggagaagt gctttcaatt cgagctgtga
      781 tgtttcggct atagaataaa aacaaacgag gtacaagagg atgtacagga accagatcaa
      841 aaatgtaaga cccaaaaact cccagcgctt tttaagtcgg agtattgcaa gtaatccatc
      901 aaacaagccg cttaccaagg catttacttt tgtgcgaact tttgggtgaa taaaatacaa
      961 aacaactccc gcaagtaaca aggcagacaa aacaacaagt gcaataaaca tccagctgct
     1021 gcttttctga tcaccggaac ccatagctga aaatgtatct ttcaattcaa tgaatttatc
     1081 gtactgtagt gcaatggtga tcaacgaaac aatccccagc ataaacacat ccaccacacg
     1141 ctctgcgagg atggttccga atgctttttc gaagggaatc ttttcgtaac gtgtcatcat
     1201 tcctgcgcga ctcacttctc cggcacgggg caaaagaaga ttcatgaaat aaccaatcat
     1261 ggtagcatgg taactgttcc acacccgcgg ctgatacccc atgggttcga gcaggtactt
     1321 ccagcgcaag gcacgcacaa aatgactgca ccaggaaaag atgaccgaaa gagcaatgaa
     1381 aaaataattg gcgcgcccca tcacctgagc caccttatcc ttgtcctgaa tcgaatcgaa
     1441 cgaataccat gccaggtaaa tccccagcgc cagcggtacg gcaatcttca ggactttcag
     1501 aacaatcttc aagcctttag cgaattggtg gcagcgtcgg ggaaaatgac atgcggacga
     1561 aagcttttcg ctttttcgaa gtccattccg gcataggaga tcacgatcac atcgtcgccc
     1621 acagtaaatt tcagcgcggc cggaccgttg aggcagatca ctccgctacc ccgttcgccc
     1681 ttgatgacat aggtaatgat gcgttcgcca ttgcggtaat tgagcacgtg aacctgttcg
     1741 ttttcaataa ggttagcagc atccatgagg ttttcatcga tggtgatgct gccgatataa
     1801 tccagattgg cttcggtaac tctgcaccgg tggatcttgg atttaagaac ctgaatttcc
     1861 atggggcaaa ggtagaagaa aatggaatgt gcgaatgaaa ggaatgtgcg aatgtgcgga
     1921 tgggggaatg tgcgaatggg aggaatatgc gaatgtgggg aatgtgggga atccttcttc
     1981 gccagggcta cgaaggataa atgtgcgaat ggggggtgtt cgaatcctga cgtttaaatc
     2041 aaaaatatct cctgaggtga aataaaggtg gaatacattt gagatagtac cgaaaaggga
     2101 ataagggaag aagagagata ttcgcctttt ccctttgtcc cttagtggct tagacttgtt
     2161 agagcatggt tgtcggacag acgtaatcgc tgatgcggaa ttttccggtt gctttaatgg
     2221 cggaaaaacc tccggctaca ttgatcaggt tgcggtaacc tctggcctgg aggatagata
     2281 cgaatgtcat tgaacggtat cctcctgcgc aatgcacgta atagcttttg tttttgtcca
     2341 ccttcagcat gctgtcgttg atgtaatcca acggtgcgtt ttcggcttct acgatgtgct
     2401 cgctgaagta ttcactggct ttgcggacat ccagaatgtt tgcattgcct tttttatacg
     2461 cgacagcaaa ttcatctgca gaaacggaag taatcgtgtc ggtttccttt gcggctttga
     2521 tccattccgc tattcctccc ttcaggtaac ccagcggctg gtcgtatcca acccttgata
     2581 aacgcgtgat gacttcttct tctcttcctt cttcaataat gagcagcaag ggttgtttaa
     2641 catcaggaac caatgtccct acccacacag caaagctgcc atcgatgccg atgttaactg
     2701 cattgggaat aaatcctttg tgaaactctt ctgcacggcg tgtatcaatg atcagcgcac
     2761 cggtttcatt ggcggcgact tcaaattcca ccggattcaa tgcacgcatt ccgcgatcca
     2821 taaccttgtc gaaactgtcg tagcctttga tgttcaacac tacattctgc ggaaaataac
     2881 cgggaggagg ggttaaacca gtaagcaatt gctttttgaa ttcctctttg cttaacgcag
     2941 gattcagcgc atagtttacc ttcttttgat tacccagggt atcatacgtt tcct
//
LOCUS       DAOMYG010000399         2989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_12186_length_2989_cov_10.238241, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000399 DAOMYG010000000
VERSION     DAOMYG010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12186_length_2989_cov_10.238241"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..876)
                     /gene="folP"
                     /locus_tag="PK610_11610"
     CDS             complement(28..876)
                     /gene="folP"
                     /locus_tag="PK610_11610"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRJ36630.1"
                     /translation="MDQDTVFPHLDSLRTGPRLLELDQVRIFGILNLSPDSFFSGSRI
                     SGHSMTIDRAGKMLEEGADILDLGAASTRPGAEEPSIQEELDRLIPAIEAIHKAYPEA
                     LISADTYRSEVAHKAHAAGAVIINDVSGGNLDEAMFRTVAELGTAYVLTHMRGNPKTM
                     NELANYNSVTYDVIRELSSKVKALHELGVHDILIDPGIGFAKNAAHNFQLIRELELFR
                     IFPQPLLMGISRKRFIWQTLNSNPEEALNGTTAMHMLCLNAGAKFLRVHDVKEAREVI
                     QLFQEG"
     gene            913..1458
                     /locus_tag="PK610_11615"
     CDS             913..1458
                     /locus_tag="PK610_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010517861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HRJ36631.1"
                     /translation="MHNTSVQYDAVINRCRDIFVKKTHDYGSAWRILRTSSLTDQIFI
                     KAQRIRTLEEVGESKVGEGIEPEFIGIINYSIMALVQLEEGIAAEAEMPADRALALFD
                     RLAAQAKALMEKKNHDYGEAWRSMRVSSFTDLILQKLLRIKQIEDNKGKTLISEGIDA
                     NYFDMVNYAIFALIKLDEGER"
     gene            1523..>2989
                     /locus_tag="PK610_11620"
     CDS             1523..>2989
                     /locus_tag="PK610_11620"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRJ36632.1"
                     /translation="MKRLLTLQGILGILFLAAAAVFLILPYKSETVVDEFNIYWMFTY
                     SALGLGFLIVIHESGLSIATLIARIFTGSLFIVSGLIKANDPKGFGYKLEEYFREDSL
                     GSFWSSFHDIAIPLAIVIATAEVVLGLAVLFGGKSKLTTWTLFGMALFFAWLTWYTAS
                     CNDNHATFQADKAERMKTVQEQCGEWWDYKDETPDASYLPEEVEGITNCKAKFLEVDT
                     LSFGRECVNDCGCFGDALKGSIGRSLTPWESFYKDITLMFFVLVLLVQQNKIKLNTLK
                     DDVVLLPASLLAIALFSGGLFSWWFPFWFTLIGIIIYLLLKRYFITKLGQDWTIAIGM
                     IIYSFTFTFYCYTYLPIKDFRPYQIGNDLMVEKTDIPPVLKFEYEFKVIATGEKVWLD
                     AFPENYEATHEYLTNKTTIVDPGRNAAARDFSMSLIETGEDITDSVLKLENVFFLVCY
                     DLSKAAPAKLKAMNTFAQAAMAEGYYFYLLTSTLPDKVN"
BASE COUNT          786 a          661 c          685 g          857 t
ORIGIN      
        1 tatctcctac aattttcccc gggaaaatca cccttcctga aaaagctgaa tcacttccct
       61 cgcttccttc acatcatgca cacgtaaaaa ttttgcaccg gcattcaggc aaagcatgtg
      121 cattgcagtg gtaccgttca gtgcttcttc cggattgcta ttcagcgttt gccagatgaa
      181 tcgcttacgt gagattccca tcagtaaggg ttggggaaaa atccgaaaca attcaagttc
      241 acgaatgagt tggaaattgt gtgctgcatt tttcgcaaat ccaattccgg gatcaatcag
      301 gatatcgtgt actcctaatt cgtgcagggc ttttactttc gagctaagct ctctgatcac
      361 atcgtaggta accgaattgt agttggccag ctcattcatc gttttcgggt ttccccgcat
      421 atgagtaagc acgtacgcag ttcccaattc tgcaactgtc cgaaacatgg cttcatccag
      481 attccctccg ctcacatcat tgatgatgac ggctcctgca gcatgggctt tatgtgctac
      541 ttccgaccgg taagtgtctg cagaaatcag ggcctccgga taggctttat gaatggcttc
      601 aatggcgggt atcaaacgat caagctcttc ctgaatactc ggttcttctg ctcccggacg
      661 cgtggatgct gcgccaaggt caagaatatc agcaccttct tcgagcattt tgccggctct
      721 gtcgatggtc attgaatgcc ctgagatgcg actgccggaa aaaaaactgt cgggactgag
      781 gtttaatatg ccgaaaatgc ggacttggtc taattcgaga agacgcgggc ctgtcctaag
      841 ggagtccaaa tggggaaaaa ctgtatcttg gtccactttt gcggattttc tgcaatatac
      901 aacatgctgg tcatgcacaa tacctctgtt caatacgacg ccgtcatcaa ccgttgtcgc
      961 gatatcttcg tcaagaagac gcacgactat ggcagtgcct ggcgaatttt gcgtacttct
     1021 tctttgaccg atcagatctt cattaaggcg cagcgcatcc gtaccctcga ggaagtgggt
     1081 gaatcgaaag taggagaggg gatcgagccg gaattcattg gtattatcaa ttactccata
     1141 atggcattgg tgcagctcga agaaggaatt gcagccgaag cagaaatgcc tgcagaccgt
     1201 gcacttgcgc ttttcgatcg actggccgca caggctaagg ctttaatgga aaagaaaaat
     1261 cacgattacg gtgaagcctg gagaagcatg cgtgtttctt cgtttaccga tttgatattg
     1321 caaaagttat tgcgcattaa acaaattgag gataataaag gaaagacact catctcggaa
     1381 ggcatcgacg ccaactattt cgatatggtg aattatgcta tttttgcttt gatcaaactg
     1441 gatgagggag agcgttaaat acatgttaaa gccggaaagg ctgttgctct ccgatgtaat
     1501 ttgtgcctaa atcaaaggaa ctatgaaacg gctacttact cttcagggaa ttctcggtat
     1561 tctgtttttg gctgctgctg cggtatttct aattctaccg tacaaaagcg aaacagtagt
     1621 ggatgaattt aacatctact ggatgtttac ctacagcgca ttgggattgg gattcctgat
     1681 cgtcattcac gaatccggac tcagcatcgc tacgctgatc gcccgcattt ttacgggttc
     1741 actttttatc gtttccggac ttatcaaagc aaacgatccc aaaggtttcg gttacaaact
     1801 cgaagaatat ttccgggaag atagtctggg ttcattctgg tcttccttcc acgatattgc
     1861 cattcctctt gccattgtaa ttgctaccgc agaggttgtt cttggtctcg ccgttttatt
     1921 cggtggaaaa tcaaaactca caacatggac ccttttcggt atggctttgt tttttgcatg
     1981 gcttacctgg tacactgcct cctgtaatga taatcacgcc acattccagg ccgacaaagc
     2041 cgaacgcatg aaaacggtgc aggaacaatg cggggagtgg tgggattaca aagacgaaac
     2101 accggatgcc agttacctgc cggaagaagt agaaggaatt acaaactgca aagcaaaatt
     2161 ccttgaagtg gacacactca gtttcggacg cgaatgtgta aacgattgcg gatgttttgg
     2221 cgatgcactg aaaggctcca tcggaagatc gctcactcca tgggagagtt tttataagga
     2281 tatcaccttg atgttcttcg tgctggtgtt gctggttcag caaaataaaa tcaaactgaa
     2341 tacactgaaa gatgatgtcg tacttctgcc tgcatcttta ttggccattg ccttgttttc
     2401 aggtggattg ttctcctggt ggttcccctt ttggtttacg cttatcggta taattattta
     2461 tttactgctg aaacgctatt tcattacgaa acttggtcag gactggacca tcgcaattgg
     2521 aatgatcatt tactctttca catttacctt ctattgctac acctacctac ccattaagga
     2581 ttttcgtcct taccagatcg gaaatgatct gatggtagag aaaactgata ttccgccggt
     2641 attgaaattt gaatacgagt ttaaagtaat tgccaccgga gaaaaagtat ggttggatgc
     2701 ttttcctgag aattatgaag ctacccatga atacctgact aataaaacta ctattgtcga
     2761 tccgggaaga aatgcggctg cgcgtgattt tagtatgagt ctgattgaaa ccggagaaga
     2821 cattaccgat tctgttctta agctggaaaa tgtattcttt ttggtgtgtt acgacttaag
     2881 caaagcggct cctgctaaac tgaaagcgat gaataccttc gctcaggcag caatggccga
     2941 ggggtattat ttctacctgc tcacttcaac actaccggat aaggtgaat
//
LOCUS       DAOMYG010000400         2971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_12275_length_2971_cov_8.484568, whole genome shotgun sequence.
ACCESSION   DAOMYG010000400 DAOMYG010000000
VERSION     DAOMYG010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12275_length_2971_cov_8.484568"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1575)
                     /locus_tag="PK610_11625"
     CDS             complement(109..1575)
                     /locus_tag="PK610_11625"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRJ36633.1"
                     /translation="MSTEAREAAAAYDSEFKNYIDRERSAVALIAAVGELMYIKSVEL
                     VLFRNHLVDRSISEVLQLHAYAREVVKKPILVHDTAQLAKEILQLDLVPSKLDIGKLA
                     AEWMEEGKTFASMSDFLKKKLQHFIGEAGAPLKPRDVVLYGFGRIGRLCARELVKQAG
                     KGQQLRLRAIVTRGSSEAEVVKRAALLRMDSVHGPFAGTVDINMTNKTLSINGQIVHL
                     IDASNPEDVDYEQYGITNALVIDNTGVFTNLEALSRHQKAKGVSRVLLTAPGKEIPNI
                     VYGVNHKDLDRESDKIYSAASCTTNAIAPILKVVNDAIGVERGHIETVHAYTNDQNLI
                     DNMHKKSRRGRSAAINMVITETGAGKAVTKCIPELKDKLTANAVRVPIPNGSLAVLSL
                     TLSKPTTMEEINGLIRAASLEGELVNQIYYSYEPELVSSDIIGNTCCSVFDSPATIVA
                     PDGKNVVLYVWYDNEYGYTKQVMRLAKYVSKVNRLVYY"
     gene            complement(1601..2464)
                     /locus_tag="PK610_11630"
     CDS             complement(1601..2464)
                     /locus_tag="PK610_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HRJ36634.1"
                     /translation="MRQLKITKSITNRESQSLDKYLQEIGKEELITAEEEVSLAQRIK
                     KGDQRALEKLTRANLRFVVSVAKQYQNQGLTLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAFSKLEQEFERPPSAEELAN
                     VLDIPEDKIADTMRVSGRHVSVDAPFVEGEDNSLLDVLVNNDSPRADQTLMSESLQRE
                     IERSLSTLTERERDVIKLFFGIGMNHGLTLEEIGAKFDLTRERVRQIKEKAIRRLRHT
                     SRSKLLKTYLG"
     gene            complement(2610..>2971)
                     /locus_tag="PK610_11635"
     CDS             complement(2610..>2971)
                     /locus_tag="PK610_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRJ36635.1"
                     /translation="IRSRKEMLIPVTLRNSKNTTEMVKKEELNLTTALGADFAEPSPL
                     EKQRLRIKGGAKVTDLRNGKLRSAGLKPGFIITRIDDVVVENPDHMMKVLKEKQGGVL
                     VEGMYPNGARGYFALGL"
BASE COUNT          777 a          681 c          682 g          831 t
ORIGIN      
        1 agcgataatg gtacattcag gtaattctat tctttgttct ttcacagcac tgtgaaaata
       61 gaaaaacccc gacaattttg ccggggttat ctgatttatg ggttgcgatc aatagtagac
      121 caaacggttt accttcgaaa cgtatttcgc aagacgcata acctgcttgg tgtatccgta
      181 ttcgttatcg taccaaacat acaacacaac gttcttaccg tcgggtgcaa caatggtggc
      241 agggctatcg aatacagaac aacaggtatt cccgatgata tcggatgaaa cgagttccgg
      301 ttcgtagctg taatagatct ggttaacaag ttcaccttcc aaagaagcag cacggatcaa
      361 accgttgatc tcttccatag tagttggctt gctcagcgta aggctcagaa cagcaagtga
      421 tccatttgga atgggtacac gaacggcatt ggctgtgagt ttatctttta attcaggaat
      481 acatttggta accgctttac ccgctccggt ttcagtaatc accatattga tggctgccga
      541 acgtccgcgt cgcgattttt tgtgcatgtt atcaatcagg ttctggtcgt tggtgtacgc
      601 atgaacagtt tcgatgtgac cacgttccac tccaatagca tcattcacca ctttaagaat
      661 gggcgcaatg gcattggtgg tacacgatgc tgcagaatag atcttatcgc tctcgcgatc
      721 gaggtcctta tgatttacac cgtaaacaat attggggatt tcttttccgg gtgcagtaag
      781 caaaacacgc gatacacctt tagccttttg gtggcgcgac agtgcttcaa gattggtgaa
      841 cacaccggtg ttatcaatga ccaatgcatt ggtaattccg tattgttcgt aatccacatc
      901 ctcaggattg gaagcatcga tcaggtgaac gatttgtccg tttatactga gggttttgtt
      961 ggtcatattg atatcgacag tacctgcgaa aggaccatga accgaatcca tacgtaataa
     1021 ggcagcacgt ttaaccactt ctgcttcgga gcttccacgg gtaacaatag cacgaaggcg
     1081 caattgctga cctttaccag cttgctttac aagttcgcga gcgcagagac gaccgatacg
     1141 accgaaaccg tacaaaacaa catcacgtgg tttaagtgga gcaccggctt caccgataaa
     1201 atgctgaagt ttctttttga ggaaatcaga catggaagcg aaggtcttcc cctcctccat
     1261 ccattcggct gcaagcttac cgatatcgag ctttgaagga accagatcca attgaagaat
     1321 ttcctttgcc agctgggcag tatcgtgaac aagaataggt tttttaacca cctcacgcgc
     1381 ataggcatga agctgaagaa cctcagaaat gctgcggtcc accaggtgat tgcggaagag
     1441 aacaagttcg actgatttga tgtacatcag ttctccaaca gcagcaatca gtgcaacagc
     1501 agatcgttcg cggtcgatgt aatttttgaa ttcggagtcg taagctgcgg cagcttctct
     1561 ggcttcggtt gacatggatc gtttgtttgt tagggttggt ttatccgagg taagttttca
     1621 gcagtttgct gcgtgaagta tgacgaagac ggcggatagc cttctctttg atctgacgaa
     1681 cacgctcacg ggtaagatcg aattttgcac cgatctcttc gagtgttaaa ccgtggttca
     1741 taccgattcc gaagaacaat ttgatcacat cacgctcacg ctcagtaaga gtggaaagcg
     1801 aacgctcaat ctcacgctgc agagactccg acatcaaggt ctggtctgca cggggtgaat
     1861 cattgttcac aagcacatcc agaagtgagt tatcttcccc ttcaacgaaa ggagcatcca
     1921 ccgacacatg gcgaccggaa acacgcatgg tgtcggcgat cttgtcttcg gggatatcca
     1981 gaacattggc caattcttct gcacttggcg gacgttcaaa ttcctgctca agtttagaaa
     2041 atgccttgtt gattttgtta agtgaaccta cctgattaag gggtaaacgc acgatacgcg
     2101 actgttccgc caaagcctgg aggatagatt gacgaatcca ccacaccgca taggaaataa
     2161 atttaaaacc acgggtttca tcgaaacgct gggcagcttt gatcaaacca agatttcctt
     2221 cgttgatcaa atcaggaagc gttaagccct gattctggta ctgttttgca acagaaacaa
     2281 cgaaacgaag gttggcacgg gttaattttt caagtgcacg ctggtcgccc tttttaattc
     2341 gctgggcaag cgatacctct tcttcagctg taatcagctc ctcttttccg atctcctgca
     2401 ggtatttatc aagtgattgc gattctctgt tggtaatgga tttggtaatc tttagctgtc
     2461 tcatcgctca atttggggta taaagtgaat gttcgtacaa atcagggtgc aaaggtagat
     2521 gaatacacgg ttaaagtcaa gttatgttca ccttattcat cgttaagatt gtagttttaa
     2581 cgtcgggaag ttcaattccg tctaaaagat cacaatccca aagcaaaata gccccttgcg
     2641 ccgttgggat acatcccttc aaccaaaaca ccgccttgct tctctttcag caccttcatc
     2701 atgtgatcgg gattctcaac gacaacgtcg tcgattctgg tgatgataaa cccgggcttc
     2761 aatccggcac tgcgtaattt accgttgcgc aagtctgtta ctttagcacc tcctttgatg
     2821 cggagacgct gcttttccaa tggactcggt tctgcgaaat cggctcccag tgcggtggta
     2881 agattgagtt cttccttttt caccatttcg gtggtgtttt tcgagttgcg taatgttaca
     2941 ggaatcaaca tttccttcct gctgcggata a
//
LOCUS       DAOMYG010000401         2957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_12352_length_2957_cov_8.670227, whole genome shotgun sequence.
ACCESSION   DAOMYG010000401 DAOMYG010000000
VERSION     DAOMYG010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12352_length_2957_cov_8.670227"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PK610_11640"
     CDS             <1..845
                     /locus_tag="PK610_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRJ36636.1"
                     /translation="YLSMRDFKTVKSNDTIYYVVGDYLSAEEAKVRQNEIEELGISTE
                     GVAQSSTKNTREGTQTTITKIPDDKLPKNTNTTQPHDKDVTFRVQVGAFTKPVSQEVF
                     KDVPGLLVVKGPDGITRYYSGSYKTFGEAAEHKLKLDMQGYSGAFVVAYKEGERKNLE
                     EVGATLSEGTKSSELNIDEKTDINKPVVDAGLVKYKIEVGRYKNDIPTEVLDMFLDLG
                     KVLPRRAENGDVVYLYGEFSTIKEAEAALKAAQNAGLKKALIIGDFNGKIISAEEAKS
                     LLRQ"
     gene            885..1157
                     /locus_tag="PK610_11645"
     CDS             885..1157
                     /locus_tag="PK610_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36637.1"
                     /translation="MKKLFIIGLFASFACITSAQQVKSDTSKVAKGQAKENTVESGKT
                     SSDTTKNQQRITVNEEGVNSKKKNAKLKNASAPAEGTPPEEKNKKK"
     gene            complement(1162..2238)
                     /locus_tag="PK610_11650"
     CDS             complement(1162..2238)
                     /locus_tag="PK610_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRJ36638.1"
                     /translation="MNRELITELIERYLEGDMSPVEKAAFEERLENDSSFLKEYELHM
                     QVRRSFSWEMDRRSLRSVMHHAHREMEGPGLSKKSSIKVKGTVSVRRIILIAASVSLL
                     ISAGSLVTYNLIQENEARSSYARLNRTSSPAESTKAAVEENAAAPANISDSEEKQQTT
                     IATAFAIHEDGWMLTNHHVVAGKKYVYVEKMADSLNRYVAEVIASDKRLDIALIRVSD
                     PAFIKLPQIPYVFGTEDAMIGQRVFTLGYPKDDIVYSEGPVSSLTGYKSDTIALQLSL
                     PVNPGNSGAPVFSENGELMGVITGKNIEADGAAYGLKVSHLREFLQNSEEYKGSLPKK
                     NLLKGKKLTDQVKRIDTFIFLVKA"
     gene            complement(2241..2828)
                     /locus_tag="PK610_11655"
     CDS             complement(2241..2828)
                     /locus_tag="PK610_11655"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ36639.1"
                     /translation="MNERSQVVISESEIRAVEAVRSGDERVIKELYKTHFQLVKRLVL
                     KNSGDEDDARDLYQEAFMVFYENVKDPDFQLKSAISTYLYSISRNLWMKKLRSWSGSP
                     VIRVEDEDMVDVEEDIALSEQKEQWAEALDRSLEKIGEPCRTLLEDFFFHRLSMEEIA
                     RKMNYTNAANAKNQKYKCFNRLKKLVLTEKRPNNN"
BASE COUNT          897 a          644 c          650 g          766 t
ORIGIN      
        1 aatatttatc catgcgcgat tttaaaacgg tgaaatcgaa cgatactatt tattatgtgg
       61 ttggagatta cctgagtgca gaagaagcga aagtgcgtca gaacgaaatt gaagaacttg
      121 gaatcagcac ggaaggagtt gcacaatcct ccactaaaaa cacacgtgaa ggaacacaaa
      181 caaccatcac aaaaattcct gacgacaaac taccaaagaa caccaacact acacaaccgc
      241 acgataagga tgtaacgttc cgcgtacagg ttggcgcatt taccaaaccc gtatcgcagg
      301 aagtatttaa agatgttccg ggattgctcg tagtgaaagg tccggatgga attacgcgct
      361 attattcggg aagctacaag acctttggcg aagcagcaga gcacaaactg aagctcgata
      421 tgcaagggta cagtggtgca tttgtagttg cctataaaga aggagaacgt aaaaatctgg
      481 aagaagtcgg tgctacgctt tcagaaggaa caaaatcaag tgaactgaac attgacgaga
      541 aaaccgatat caacaaaccg gttgtagatg caggcttggt gaaatataaa attgaagtag
      601 gccgttacaa aaacgacatt ccaactgaag tactcgatat gttccttgat cttggaaaag
      661 tattaccccg tcgcgcagaa aacggtgatg tagtgtattt gtatggagaa ttcagcacca
      721 ttaaggaagc cgaagcagca ttgaaagcag cgcagaatgc aggattgaaa aaggcattga
      781 tcattggtga tttcaacgga aaaattattt cagcagaaga agcaaaatcc ttacttcgtc
      841 agtaacggca taagctttgc aatagaattc aaaattagca gcacatgaaa aagctattta
      901 ttattggttt gtttgcatcg tttgcatgca tcacctcagc gcagcaagtg aaatccgaca
      961 cctcaaaagt tgcgaaggga caagcaaagg agaacactgt agaaagcgga aaaacaagca
     1021 gcgacaccac aaaaaatcag caacggatta cggtgaatga agaaggtgtg aacagcaaga
     1081 agaaaaacgc aaagctgaaa aacgcatcag cgcctgcaga aggaactcct ccggaagaaa
     1141 agaataagaa aaagtaaggg attaagcctt taccaggaag atgaaggtat caattcgctt
     1201 cacctggtcg gtcagctttt tacctttcag taaattcttc ttcggcaaac ttcccttgta
     1261 ttcctcactg ttctgcaaaa actcccgcag gtgagaaact tttagtccgt atgccgcacc
     1321 atcggcttca atatttttac cggtgatcac acccataagt tcaccgtttt cggagaatac
     1381 cggagcacct gagttacccg gatttacagg taatgaaagt tgtaaagcaa ttgtatcact
     1441 tttgtaaccg gtcagtgaac ttacaggacc ttcgctgtaa acaatatcat ctttcggata
     1501 tcccagagtg aaaacgcgtt gtccgatcat tgcatcttca gtcccgaata catagggaat
     1561 ctgtggtaat ttaatgaatg caggatcgct tacacgaatc aatgcgatat cgagacgctt
     1621 atctgaagcg ataacttctg ccacgtagcg attcaggctg tcggccattt tttcgacgta
     1681 aacatatttt tttcctgcaa ccacatgatg gttggtcagc atccatccat cctcatgaat
     1741 tgcgaatgcg gttgcgatgg ttgtttgttg tttttcttca gaatcagaaa tattagccgg
     1801 agcagcggca ttttcttcaa cagcagcttt cgtagattcg gcaggcgaag aagtccggtt
     1861 aagtcgggca tacgaagatc tggcctcgtt ttcctgaata aggttatagg ttaccaaaga
     1921 acctgcagag atcagaagag aaacagaagc agcgatcagg ataattctgc gtacagaaac
     1981 ggtacctttt acttttatgc tacttttctt agacagaccg ggaccttcca tttcccggtg
     2041 agcatgatgc ataaccgaac gaagagagcg acgatccatc tcccatgaaa aactccgacg
     2101 aacctgcatg tgaagttcat attcctttaa aaaggaggaa tcattttcca aacgttcttc
     2161 aaaggcagcc ttttcaacag gcgacatatc accttcgagg tagcgctcta tcagttcggt
     2221 aatcagttcg cggttcatat tcaattatta ttcggccttt tttcggtcag caccagtttt
     2281 ttcagccggt taaagcattt gtatttctgg ttcttcgcgt tggccgcgtt ggtatagttc
     2341 atttttcggg cgatttcttc catactcagc cggtgaaaaa agaaatcctc cagcagcgta
     2401 cgacagggtt cgccaatttt ttcaagtgaa cgatcaagtg cttcggccca ttgctccttc
     2461 tgttctgaaa gcgcaatatc ttcctcaaca tcaaccatat cctcatcctc cacgcgaatc
     2521 accggcgagc ccgaccatga gcgcaatttt ttcatccaga gatttctgga gatgctgtac
     2581 aaataggtag atatggctga tttaagctga aaatcaggat cttttacatt ttcgtaaaag
     2641 accataaatg cctcttggta caaatcgcgc gcatcgtctt catcacccga gtttttaagg
     2701 accaatcgct taaccaattg gaaatgcgtt ttatacaact ccttaatcac ccgttcgtca
     2761 ccggatctta cagcttctac tgcacggatc tcggattctg agattacgac ttgtgaacgt
     2821 tcattcatat atacctaaag acagtgcaag gtaacccaat tttttttttt gaatatgggt
     2881 tacctccatc ggtatttggg tactggccgc gctggcgctg gccgaagcgg cgctgaaatc
     2941 ggtggccgag gggcggg
//
LOCUS       DAOMYG010000402         2956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_12361_length_2956_cov_7.969321, whole genome shotgun sequence.
ACCESSION   DAOMYG010000402 DAOMYG010000000
VERSION     DAOMYG010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12361_length_2956_cov_7.969321"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /locus_tag="PK610_11660"
     CDS             complement(<1..1599)
                     /locus_tag="PK610_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36640.1"
                     /translation="MKKLTPFSVLLQHFEKAQRQFGNDANRQTLSALEELQSCKLDNP
                     KLFQRYADALLFTVAYPGSEDIAFAAEGEMGRLFRACEKWSGKKGAEALYNSALPGTE
                     VCATYSLTHNEWLLNRYGKNVSLYEEDLNTEEIIKHLQHALDPVELEVYNEGLDDWDT
                     WKNEIAGFKEDAHRTLDFLVGQTALLKGTLGWKESLFSSFHTFTNWQTKKDDPLFYGA
                     RIHFSPSFIHRSGILKKMTIGEALEQGQPKAIKLNPEDRNKLVDIGKAVLGSLMRETD
                     PITYAQVQETEYFDMGRGVGIALYYMVPEMKMTLQSYVGYLLFKNGAPCAYGGGWLFN
                     NESGFGVNIFPPYRGGESANIVCQLLRLYALHFNANTFTVDPYQIGLGNPEGIQSGSF
                     WFYYRLGFRPEQSELRKLAESEFKKIAAKTDYRSNEKTLLRLAHSTMRWTHPDRKEKN
                     FLSAEKIAEKTRTHINKKHQGDRGKATVSALIKIRENTGRDHFNPLAAKIATTLDAAG
                     LLDKTDPEILNEFLEAYRLKSQYER"
     gene            1651..>2956
                     /locus_tag="PK610_11665"
     CDS             1651..>2956
                     /locus_tag="PK610_11665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRJ36641.1"
                     /translation="MFNRKKYRPVEAWSFWGKLKLTVLRMAFTLRRLLGGRDWDAVWP
                     EHHEDPLHMKRRELIYFSHKYYYRAIIKADPGSGLETHFRSHAGSLCNDAEFIPEKVI
                     TIHSGGDLMPYASINAGVCKGLWDEVGEEFFSADIVTANLETPVVLNKKPGLVPEVML
                     NHMYFNASEEMMEVFSGNKKHKGYDVLSVANNHSLDQGTQGLEATLRMLREKNIAYCG
                     ARHLPDDLHAVPILDRNGIKVAFLAATFSLNAEQLLHGEEWRVDHLPLNKSNPDISRL
                     VLQAKNARAKGADLLVAHLHMGCAYQPYPSMHTVTNMREICRQTGIDVVLGGHPHNPQ
                     PLEYFLFIDPFTGIEKQSTIIYSMGDFVAYDIFKWCHLPLVVKMKIGKRGNETRVLQV
                     IPQLWYMQAEKSGGAIESLKFKKADDFLNPDNIELEELKKFRK"
BASE COUNT          804 a          594 c          670 g          888 t
ORIGIN      
        1 gcgttcgtat tgcgatttga gtctgtacgc ttccagaaat tcattcagga tttcaggatc
       61 tgttttatcc agtaaacctg ccgcatcaag tgtagttgca attttcgcag caagcggatt
      121 gaaatgatct ctaccggtat tttccctgat ttttattaat gcgctaactg ttgcttttcc
      181 acgatcgccc tgatgttttt tgttgatgtg ggttcgtgtt ttttcggcaa ttttttcagc
      241 tgagaggaag ttcttttcct tacgatcggg atgtgtccaa cgcatggtgg aatgagcaag
      301 ccttaaaagt gttttttcat tgctgcggta atcggttttt gcagcaattt ttttgaattc
      361 gctttccgca agtttgcgta attcggattg ttccggacga aaaccaagtc ggtaataaaa
      421 ccaaaaactg cccgactgaa ttccttcagg attaccaaga ccgatttgat aggggtcaac
      481 ggtaaaagtg ttcgcattaa aatgaagtgc atacaaacgc agcagctgac aaacaatatt
      541 tgccgattca cctccgcggt aaggcggaaa aatatttact ccaaatcctg attcgttatt
      601 gaacaaccat cctccgccgt atgcgcaggg agctccgttt ttgaacagga gataacccac
      661 gtaagattgc aaggtcattt tcatttcagg aaccatgtaa tacaatgcga ttccaacgcc
      721 tctgcccatg tcgaaatatt ctgtttcctg cacttgcgcg tatgttatcg gatcggtttc
      781 ccgcatcaga gatcccagaa ctgcttttcc aatatcaacc agtttgtttc ggtcttcggg
      841 attaagtttt attgcttttg gctgaccttg ttccagcgct tcgccaatgg tcattttttt
      901 cagtatgccg cttcggtgaa taaacgaagg agaaaagtga atacgcgctc cgtaaaaaag
      961 aggatcatct ttttttgttt gccaattcgt aaaagtatgg aaagaagaaa agagcgattc
     1021 tttccagcca agagttcctt tcagcaatgc tgtttgtcct actaaaaaat ccagcgtgcg
     1081 atgggcatct tctttaaatc cggcaatctc atttttccag gtgtcccaat catcgaggcc
     1141 ttcgttgtaa acttccagtt caaccggatc gagtgcatgt tggaggtgtt tgataatttc
     1201 ttcggtgttg aggtcttctt cgtagagcga aacatttttt ccataacgat tcaacaacca
     1261 ttcgttgtgg gttaacgaat aagtagcgca tacttctgtt cccggtaatg ctgagttgta
     1321 taatgcttct gctccttttt taccactcca tttttcgcag gcacgaaaaa gacgacccat
     1381 ctctccttca gctgcaaatg cgatatcctc gcttcccgga taggcaactg taaaaagcaa
     1441 agcatcagca taccgttgaa aaagtttggg attatcgagt ttgcacgatt gtaactcttc
     1501 caaagcactt agcgtttgcc tattggcatc gttgccaaat tgccgttgtg ctttttcaaa
     1561 gtgttgaagt agtacagaaa aaggagtcaa ttttttcatt ggatggagga gggattaatc
     1621 ctatttttga gtgaattaaa tgtaagggat ttgttcaaca gaaaaaaata ccgtcccgtt
     1681 gaagcctggt cgttttgggg taaactcaaa ttgacggttt tacggatggc gtttaccttg
     1741 cgacgcttat taggcgggcg cgactgggat gctgtttggc ccgagcatca tgaagacccg
     1801 ctgcatatga agcgcaggga gttgatttat tttagtcaca aatactatta cagagccatt
     1861 atcaaagccg atcccggatc tggattggaa acacattttc gttcgcatgc cggatcgcta
     1921 tgtaatgatg cagaatttat tccggaaaag gtgattacaa ttcattccgg tggcgatctg
     1981 atgccttatg cttctatcaa tgccggggtt tgtaaagggc tttgggatga ggttggggag
     2041 gagttctttt ctgcggatat cgtcactgcg aatctggaaa cccctgttgt actcaataaa
     2101 aaaccgggac ttgtacctga agtcatgctg aaccacatgt attttaatgc aagtgaagaa
     2161 atgatggagg tgttttccgg aaacaaaaaa cacaagggtt acgatgtgct ttctgttgcc
     2221 aacaatcatt cacttgatca gggaacacag ggattggaag caacgctacg gatgttacgt
     2281 gaaaagaaca ttgcctattg cggagcgcgt catctgcccg atgatttgca tgctgttccg
     2341 atactggatc gaaacggaat taaagttgcg tttcttgcag ctaccttttc actcaacgca
     2401 gaacaattgt tgcatggtga agagtggaga gtggatcatt tgccattgaa caaatccaat
     2461 cctgatattt cacgtttggt gcttcaggca aaaaatgcga gagcgaaagg tgcagatctt
     2521 cttgttgcgc atcttcatat gggttgtgcg tatcaacctt atccttcaat gcatacggta
     2581 accaatatgc gggagatttg ccgccaaacc ggaatagatg tcgttctggg cggacacccg
     2641 cataatccgc aaccattgga gtatttttta tttatcgatc cctttacggg cattgaaaaa
     2701 caaagcacga tcatttattc aatgggtgat ttcgttgcat atgatatttt caagtggtgc
     2761 catttaccac ttgtagtgaa aatgaaaatc ggaaaaagag ggaatgaaac aagagtgttg
     2821 caggtgattc cgcaattatg gtatatgcag gccgaaaaaa gcggcggagc aattgaatca
     2881 ttaaaattca aaaaggccga cgatttttta aatccggata atatagaact ggaagaacta
     2941 aaaaaattca gaaaga
//
LOCUS       DAOMYG010000403         2948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_12424_length_2948_cov_8.286899, whole genome shotgun sequence.
ACCESSION   DAOMYG010000403 DAOMYG010000000
VERSION     DAOMYG010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12424_length_2948_cov_8.286899"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PK610_11670"
     CDS             complement(<1..399)
                     /locus_tag="PK610_11670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ36642.1"
                     /translation="MKWFVVPFDELSPNDLYDLLELRTNVFVVEQNCPYPELDRKDQK
                     AIHVFARDQNGEIVAVARIFEPGISYSEASIGRVCTSSAVRGSGAGIALMELCIREIE
                     QRYGKCAIRISAQEYLKKFYSSFGFVQVSET"
     gene            complement(396..1148)
                     /locus_tag="PK610_11675"
     CDS             complement(396..1148)
                     /locus_tag="PK610_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36643.1"
                     /translation="MFSEIYTSKQPAPLQSYALMACSLGVTVATLVFPNLQHVFGSFD
                     EGTDFMQRLTVAFQHGFEWRSSVVHLLIDLVLMAFIAVFTEKVLGAWMFFLLSISTLA
                     WYSLVHTVFGMMGHGGSGLIWAFAPVVFYVLNEGRLLKTRSQFDELFRTFRLILAIMY
                     LVIPVLMSIIPIYFDSETPLLRSIIYGNLFHITATLWGIGFTMLFREQIRVRLKQFAK
                     KKKFEPLESDRYAAFLPWFYPLLIVLGFIFSR"
     gene            complement(1148..>2948)
                     /locus_tag="PK610_11680"
     CDS             complement(1148..>2948)
                     /locus_tag="PK610_11680"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRJ36644.1"
                     /translation="SENESLMRQKQWLFNDQFEFHTLDTNYRSGKVIVDFNNSFFSAF
                     RQQFPHALASVYDHLEQKVPNNRDYGFVSIRFSSKEMKRKQLIEEHAAWLLQSIETMR
                     VQGYAYSDICVLVRSNAEGDHMAAALARAGVPVVSPDSLLIRTSQKVQMIASVIRWMT
                     RPEDEENNGKLAELLIHHCLPGSDFYPVLDKASTEYKGRRTIDIAKLFEVLQLKLNTD
                     DMLRFSLYQLAEYLISSLSLERDEAFMNAFLDQVFSFSQRENDLHAFEEWWKEKSEKL
                     SVQLPDSSDAVKVMTIHKSKGLQFPVVMMPLLTWWDDNKKGKQRLWVELDPVFDPLQN
                     ALFKTSQETFSQIGKKEIYEEEAARTELDRINSLYVAFTRAEEQLLVFSQHLSRGWAS
                     SLIKNLIPEISDETSEYTLGTSERIKNSKRSDASMVVLKPSRPVNWQERLRISYESQR
                     AWGESAGMVSIRLGNLVHLVLSKLKNIAEIDTVLRSLETEIAAAGTTYDGVKAEMDRI
                     FSHPFLRESFAEGQNAVSELELIDASGTMWRPDRVVFFKDRTVVIDFKTGKQREEHKQ
                     QVAHYGKLLSEMGCPSVENYLFYTFDSILEKVA"
BASE COUNT          917 a          669 c          564 g          798 t
ORIGIN      
        1 ggtttcgctt acctgaacaa agccaaatga ggaataaaat tttttcaggt attcctgggc
       61 agaaatccgg atggcgcatt tcccgtaacg ttgttcaatt tcccgaatac aaagctccat
      121 taaagcgata ccggcacctg aacctcttac agcagaagat gtacaaactc tgccaatgga
      181 agcttcggaa tatgaaatac ccggttcaaa aatgcgtgcc actgcaacaa tttctccgtt
      241 ttgatctctt gcaaaaacat ggatcgcctt ttgatccttt cggtccagtt ccggataagg
      301 acaattttgt tccaccacaa aaacattagt acgaagttcc agcagatcgt ataggtcatt
      361 cggagacaat tcatcaaaag gaacaacaaa ccacttcatc tggagaaaat aaatccaagt
      421 acgatcagca aaggataaaa ccagggaaga aatgcagcgt aacgatcact ttcgagaggt
      481 tcgaatttct tcttttttgc aaactgtttt aagcgaacac ggatttgttc acgaaataac
      541 atggtaaaac caattcccca aagcgttgcg gtgatatgaa agagattgcc gtaaataatt
      601 gaccgcagca agggtgtttc agaatcgaaa tagatcggaa tgatcgacat cagtacagga
      661 atcaccagat acattatggc aaggatcaat cggaaagtgc gaaaaagttc atcgaactga
      721 cttctggtct ttagaagccg accttcatta agcacataaa aaaccacagg cgcaaacgcc
      781 caaatcaagc cacttccacc atgccccatc attccgaaaa cagtatgtac aagcgagtac
      841 catgcaagag tggaaatgct aagcaggaaa aacatccagg ctccaagaac tttttctgta
      901 aagactgcaa taaaagccat caggaccaaa tcaataagca ggtgaacaac ggatgatcgc
      961 cattcaaatc catgctgaaa tgcaactgtg aggcgctgca tgaaatctgt tccttcatcg
     1021 aaacttccaa acacatgttg cagatttgga aacaccaatg tagcaacagt tacaccaagc
     1081 gaacaagcca tcaatgcata cgactgcaat ggggcaggtt gtttactggt ataaatttct
     1141 gaaaacatca ggcgactttt tcaagtatgg aatcgaaggt gtaaaatagg taattttcaa
     1201 cagaagggca tcccatttca gaaagtaatt taccgtagtg agcaacctgc tgtttgtgtt
     1261 cttcgcgttg ttttccggtt ttaaaatcaa taactacagt acgatccttg aaaaacacaa
     1321 cacgatccgg cctccacatt gtacctgagg catcgattaa ttcaagttct gatacagcat
     1381 tttgtccttc agcaaaagat tctctcaaaa aaggatggga aaagattcta tccatctctg
     1441 cctttacccc atcataggta gtaccggcag cagcaatttc cgtttcaagt gaacgaagga
     1501 cagtatcgat ctctgctata ttcttcaatt ttgacaatac caaatgaaca agatttccta
     1561 gccggataga aaccattccg gcactttctc cccatgcacg ttgcgactca taactgatac
     1621 gtaaacgttc ctgccaattt acaggccttg aaggtttgag cactaccatt gatgcatcac
     1681 ttcgttttga attttttatt ctttctgatg ttcccaatgt gtattcgctg gtttcatcgc
     1741 tgatttcggg aatcaaattt tttataagcg aagaagccca accacgggat aagtgttgtg
     1801 aaaaaaccaa taattgttct tcggcccggg taaacgcaac atagagtgag ttgatccgat
     1861 caagctctgt acgtgcagct tcttcctcat aaatctcctt tttccctatc tgtgaaaatg
     1921 tttcctgact ggttttaaaa agagcgttct gcagtggatc aaaaacagga tccagctcaa
     1981 cccataatcg ctgttttccc ttcttattat catcccacca ggtaagcaat ggcatcatca
     2041 ccaccggaaa ctgcaaacct tttgatttgt ggatggtcat caccttaaca gcatccgacg
     2101 aatccggtaa ttgcacggag agtttctctg atttttcctt ccaccactct tcgaacgcat
     2161 gtaaatcatt ttcccgttgc gaaaaactga atacctgatc gagaaatgca ttcatgaatg
     2221 cttcatctct ttcaagcgat aaggatgaaa taaggtattc tgccaactga tacagcgaga
     2281 accgtaacat atcatccgtg ttgagcttta actgcagcac ttcaaataat ttggcaatat
     2341 caattgtccg cctgcctttg tattctgttg acgctttatc caacaccgga tagaaatcac
     2401 tacccggaag acagtgatga ataagtaatt cagcgagctt gccattgttt tcttcatctt
     2461 ccggacgagt catccatcgg attacagaag ctatcatttg aactttctgt gaagtacgaa
     2521 tcaataaact atccggagaa acaaccggaa ctcccgcacg agccaatgca gcagccatat
     2581 gatctccctc cgcattggat cgcaccagta cacaaatatc agaatacgca tatccttgca
     2641 cacgcattgt ttcaatactc tgaagcaacc aggcagcgtg ttcttcgatc agttgtttcc
     2701 gtttcatttc ttttgatgaa aaacgaatgg aaacaaatcc gtaatctctg ttgttgggga
     2761 ccttttgttc gagatgatcg taaaccgatg caagcgcatg gggaaactgc tggcgaaagg
     2821 cagaaaaaaa tgaattatta aagtcgacaa ttaccttgcc tgaacgataa ttggtatcaa
     2881 gcgtatgaaa ttcgaattgg tcattaaata gccattgttt ttgccgcatc aaggattcat
     2941 tctcgcta
//
LOCUS       DAOMYG010000404         2926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_12538_length_2926_cov_7.890979, whole genome shotgun sequence.
ACCESSION   DAOMYG010000404 DAOMYG010000000
VERSION     DAOMYG010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12538_length_2926_cov_7.890979"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1240)
                     /locus_tag="PK610_11685"
     CDS             complement(170..1240)
                     /locus_tag="PK610_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019385975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRJ36645.1"
                     /translation="MKRISVLSVFILIVVLIACRKDVFIGEYETTPYQLRIPQGFPQM
                     VIPVNNPMTVEGVELGRKLFYEELLSADNTQSCASCHLQSNAFADPRRVSVGITGAEG
                     TRNAMALINLGWEQFFFWDGRSATLEDQIFEPIRNPVEMNDTWPNVERKLRASEEYRD
                     LFHKAFGTPGIDSVRTVKAIAQFLRTLISGNSKFDKVARGQASFTPSEANGFDLFMRD
                     KDDNNGISGGDCFHCHGPILMAKQLLANNGLDTIFIDPGKAGVTFNPSDVGKFKTTTL
                     RNIELTGPYMHDGRFATLEEVINHYSSGVKVSPTIDPLMKFAADGGVGLTPQEKIDMK
                     NFLLTLTDWDFINDPAFSDPDL"
     gene            complement(1261..2262)
                     /locus_tag="PK610_11690"
     CDS             complement(1261..2262)
                     /locus_tag="PK610_11690"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRJ36646.1"
                     /translation="MKPTFFIAILLLTLASCKREEDPVLSDRYELRVPPGFPNPNIPA
                     DNYISKSRIELGKKLFFDKRLSSDNTISCASCHFPQNAFSDVSPLSVGVQGRIGFRNA
                     PPLFNLAWMPLLMKDGGVPTLELQVHAPISDIAEMDSDILQVIDEIKNDYNDLSLYAY
                     KRPFDTYVLTRALATFQRTLISGESRYDSYIIHGGSLTASELRGKDLFFADSLHCADC
                     HSGFNFTSFNFENNGLSLNHPYTGRARITLLPQDVGKFKVASLRNVEVTAPYMHDGSL
                     GTLEDVIDHYASGGQPHANKSPLITGFSLSNQDKSDLIAFLKTLTDHRFLNNPSFRP"
     gene            complement(2262..>2926)
                     /locus_tag="PK610_11695"
     CDS             complement(2262..>2926)
                     /locus_tag="PK610_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36647.1"
                     /translation="TLQVNFTASFNGQPMVLNSVYSSALNYRIKPETVKYLLHHFQLK
                     RTDGSYYEIREAVNINHANATNGFSIQLEPANYTAVKFGLGVDASMNNADPNLLPPSH
                     PFSTNQANDLHWNWSTGYIFAKFEGRADTSGTGSGNLDRLFFFHPGANTLYSEVELTS
                     SFGITTGQTRTITIDLDVNKLITNTSDTIDLKYDFSTHTNDNYPLAERFILLMRNAFS
                     IQ"
BASE COUNT          846 a          591 c          630 g          859 t
ORIGIN      
        1 ccggtaagtg caacaacaat tgaataggat tcttcttcaa gtcctttttt aataaagtta
       61 actacgctga tctcgtcttc tatcagaaga attttttgca tggaaaaatc gctattgttt
      121 ccatgaatat aggtaaaagg aacgagaacg ggaaaattga aggcttctgt tacaaatcag
      181 gatcgctgaa tgccgggtca ttgatgaaat cccaatctgt caacgttagc aggaaatttt
      241 tcatgtcgat tttttcctgt ggtgttaatc caacaccgcc atctgctgca aatttcatca
      301 agggatcaat ggtaggtgaa acttttacac ccgaactgta atgattaata acttcttcaa
      361 gcgtcgcaaa acgcccatca tgcatatatg gaccagtaag ctcaatattg cgtagcgtag
      421 tggttttaaa ttttccgaca tcactcggat taaacgttac tcccgcttta cccggatcaa
      481 taaaaatagt gtcaagtcca ttgttcgcta aaagttgttt tgccatcaag atcggaccat
      541 ggcaatggaa acaatcgcct ccggatattc cgttgttgtc gtctttgtcg cgcataaaga
      601 gatcgaatcc atttgcctca ctgggagtaa aggaggcttg tcctcgtgca accttatcaa
      661 acttggaatt acctgaaata agtgtgcgca aaaattgtgc aatggctttc acagtacgaa
      721 cagagtcaat acccggtgtt ccgaatgctt tatgaaacaa atctctgtat tcttcactgg
      781 cacgaagctt acgttcaaca tttggccagg tgtcgttcat ctccaccgga tttcggatgg
      841 gttcaaaaat ctgatcctcg agggttgcag aacgcccgtc ccaaaagaaa aattgttccc
      901 agccaaggtt gatcaaggcc atcgcattgc gtgttccttc tgcaccggta attccaacgg
      961 aaacacggcg cggatctgca aatgcattcg attgcaaatg acaacttgca cacgattgtg
     1021 tgttgtctgc cgataatagt tcttcgtaga ataattttct tcccaattca accccttcaa
     1081 cggtcattgg gttatttaca ggaataacca tttgaggaaa tccctgagga atgcgcagct
     1141 gatagggagt tgtttcgtat tcaccaatga aaacatcctt gcggcaggca atcagcacca
     1201 caataagaat aaacaccgat aaaactgaaa tacgcttcat gactgcaagt taaaaatctt
     1261 ttaagggcga aatgacgggt tatttaaaaa tcgatgatcg gtaagtgttt tcaaaaatgc
     1321 aatcagatcg cttttatcct gattgctgag tgaaaagccg gtaatgagcg gactcttatt
     1381 cgcatgcggt tgaccacctg aagcgtaatg atcgatgaca tcttccagtg ttccaagact
     1441 accatcatgc atataaggtg ccgtgacttc cacatttcgc aatgaagcta ctttgaattt
     1501 gcctacatct tgcggaagta aggtaattct ggctcttcct gtatatggat gattgaggga
     1561 taaaccgtta ttttcaaaat tgaaacttgt aaaattaaat ccgctgtggc aatctgcaca
     1621 atgcagcgaa tctgcaaaaa acaaatcctt accacgtaat tccgatgcag taagcgatcc
     1681 gccatggatt atataactgt cataacgtga ttcgccgctt atgagtgtcc gttgaaaagt
     1741 tgctagtgca cgagtgagaa catacgtgtc gaatggacgt ttgtaagcat agagactcaa
     1801 atcgttgtaa tcgtttttga tttcatcaat tacctgcaga atatccgaat ccatttctgc
     1861 aatatctgaa atgggggcat gcacttgtaa ttccaatgtt ggaactccgc catctttcat
     1921 aagaagcggc atccaagcga gattgaataa tggcggtgca tttctgaaac cgattcttcc
     1981 ctgtacgcct acacttaagg gactcacatc cgaaaatgca ttttgaggga aatgacaaga
     2041 tgcgcagctg atggtattgt cggatgataa tcgtttatcg aagaatagtt ttttacctaa
     2101 ttcaattctc gatttggaaa tataattatc agccggaata ttcggattgg gaaatcccgg
     2161 aggaacacgt aactcatagc gatcgctcaa tactggatct tcctctcttt tgcaggaggc
     2221 aagcgttagc agaagtatag ctatgaaaaa agttggtttc attattgaat ggagaaagca
     2281 ttgcgcatga gtagaatgaa acgttcagct aatggatagt tgtcgttggt atgtgtcgaa
     2341 aaatcgtatt tgagatcaat agtatctgat gtattggtga tgagtttatt tacatcgagg
     2401 tcaatggtta tggttctggt ttgcccagtg gtaatgccaa atgacgatgt caattccact
     2461 tcactgtaaa gtgtattggc gcccggatga aaaaagaaaa gacgatcgag atttcctgaa
     2521 cctgttccac tggtgtcggc gcgaccttca aattttgcga agatgtatcc cgtactccag
     2581 ttccaatgaa gatcattggc ctggttagtt gaaaacggat gagatggggg taataaattg
     2641 ggatctgcat tgttcatgct tgcatctaca cccaatccaa atttcactgc cgtataattc
     2701 gctggttcga gttgtatgct aaatccgttt gttgcattcg cgtgattgat attcactgct
     2761 tcacggattt cataataact tccgtctgtt cttttcagtt gaaaatgatg cagcagatat
     2821 tttacggttt ccggtttaat gcggtagttc aatgcacttg aataaacaga attcaggacc
     2881 atcggctgcc cgttaaagga ggccgtaaag ttcacttgca aggtgc
//
LOCUS       DAOMYG010000405         2913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_12633_length_2913_cov_7.620364, whole genome shotgun sequence.
ACCESSION   DAOMYG010000405 DAOMYG010000000
VERSION     DAOMYG010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12633_length_2913_cov_7.620364"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PK610_11700"
     CDS             complement(<1..590)
                     /locus_tag="PK610_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36648.1"
                     /translation="MERSKQFFEECQATFMTIARLILKVKWRKTFRNTGEEKLLLLGN
                     GPSLSNSMNQLGDSLGRIKTMCVNLFPTTPDFKRVKPSYFVVSAPEFWLENVDPVYLE
                     SRSSILRALRDDTSWPLEVFIPWEARRFKEWVQLPSKNPNITVTWYNIHPAEGFRWFR
                     HWAFRMQLGMPRPHNVLIPSMMVAIAKGIREVYVVGA"
     gene            complement(594..1511)
                     /locus_tag="PK610_11705"
     CDS             complement(594..1511)
                     /locus_tag="PK610_11705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ36649.1"
                     /translation="MKKPRVSVIICTHNREMYLNRAMECVALQNADSSLFELIVVNNN
                     STDSTDSICKEFRARYTNLNFSYIIEMEQGLSHARNRGIKEAKGDLIVFLDDDAFAEP
                     DFVKNLIAFYDLHPPVLSTGGKTVPLFEEEKPAWMSRFLLPLVAAMDLGPEARLFPKG
                     WYPIGANMSFRKAAVELIGNFDVLLGRKGKNLQGGEEKDFFNRLKSKGHEPWYLPTTE
                     VRHIIPASRMSINYIRRQARGIGYSERVRTKSLGLFSYWGKCFSEKMKWIASIVLFLF
                     YLITLRAQKAVFLLRFRFWVSQGLFFGRE"
     gene            complement(1513..>2913)
                     /locus_tag="PK610_11710"
     CDS             complement(1513..>2913)
                     /locus_tag="PK610_11710"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRJ36650.1"
                     /translation="GGFLLLVVLVPQSFISFLSESDLTAFSNERNPIVSDLQSQLVDY
                     RVGVFRADALRSFGFILAGFLLLYFYITKSFSRNLLVAGLGVLIIADLYSFDRRYLNN
                     EKENGQYVSWQKKGDYPYYPTEADMSVLQQEATADPSLMTKIQQEMSNVKVEKGQNLE
                     LAQIVDGAFPALNFASNYRVLEIGDPFNSSRTSYFHKSVGGYHGAKMKRYQELIEFAI
                     RREHNMAYGFLMQVAQNNVPANQIPSVLMMSGLFERTHVLNMLNTKYLILDSSIPAAL
                     NPKAKGAAWFARNVRFVENANEEVLAVANLEPAETLQVARNGKTIDSVTFRNTNYAIE
                     DSVLVDRRFAEKVKNQDPGFDSMAFVKVESYLPNQIIYKSNSSEDGFVVFSEIYYDKG
                     WTAYLDGNPVDYVRVNYILRGMDVPKGEHTIEFKFEPVGYYSMKSINLTGNLLVLLFV
                     GLFVFLEFRKPKKAVA"
BASE COUNT          887 a          671 c          535 g          820 t
ORIGIN      
        1 gcaccaacaa cgtaaacttc acgaatacct ttggcaatgg ccaccatcat ggaaggaatt
       61 agaacattat gtggtcgagg cattccgagt tgcattcgaa aagcccaatg ccgaaaccag
      121 cgaaatcctt ctgcaggatg gatattatac caggtaacag taatattagg atttttagaa
      181 ggaagctgaa cccattcctt aaaccgacgc gcctcccatg gaatgaacac ttccaatggc
      241 cacgatgtgt cgtcccgaag tgctctgaga atggatgaac ggctctccaa atacaccgga
      301 tcaacatttt ccaaccaaaa ttcaggtgcc gaaaccacaa aataagatgg cttaacacgt
      361 ttaaaatcag gtgtagtcgg aaacagattc acgcacattg tctttattct tcctaagcta
      421 tcacctagtt gattcatact gttgctcaat gagggtccgt tcccaagcaa cagcagtttt
      481 tcttcacctg tattacgaaa tgtttttctc catttcactt ttaaaatcaa acgagcaatc
      541 gtcatgaacg tagcctgaca ttcctcaaaa aattgtttcg atcgttccat gcgttactcc
      601 ctcccgaaaa ataatccctg actaacccaa aatcgaaaac gtagcagaaa aacagctttc
      661 tgtgctctta gggttatcag gtaaaaaaga aataaaacaa tacttgcgat ccatttcatc
      721 ttctcggaaa aacattttcc ccaatacgaa aacagaccca gcgattttgt gcgcacccgc
      781 tccgaataac ctattccacg cgcttgccgg cgaatatagt taatgctcat tctggaagca
      841 ggtataatat gcctgacctc agtagttggt aaataccaag gttcatgtcc tttgcttttt
      901 agccggttaa aaaaatcctt ctcttcacca ccttgtaaat tctttccttt tctacccagt
      961 aacacatcaa aatttccaat caattccact gcggctttcc tgaaggacat atttgctcca
     1021 ataggatacc atcccttggg aaacaagcgg gcttccggac ccaaatccat tgcagctaca
     1081 agaggcaaaa ggaaacggga catccatgcg ggtttttcct cttcaaataa gggcaccgtt
     1141 tttccaccgg tactaagtac aggtggatgc aaatcgtaga aagcaattaa attttttaca
     1201 aaatcgggct ctgcaaaagc atcgtcatcc agaaaaacaa tcaaatcgcc ttttgcttcc
     1261 ttaattcctc tgtttctcgc atgggatagc ccctgctcca tttcaatgat ataggagaaa
     1321 tttaaattcg tatatcgtgc tctgaattct ttacaaattg aatctgttga atctgtgctg
     1381 ttattgttca caacaatcaa ttcgaaaagt gaagaatctg cattttgcaa agcaacgcac
     1441 tccattgcac gattcaggta catttcacgg ttatgcgtgc agatgatgac cgagaccctc
     1501 ggttttttca tttcaggcaa ctgctttttt tggttttctg aattcgagga atacaaataa
     1561 cccaacaaac aataagacca ataaattacc ggtaagattt atcgatttca tggagtaata
     1621 gcccacaggt tcaaacttga attcaattgt gtgttcccct tttggcacat ccatcccgcg
     1681 aaggatataa ttcaccctga cataatcaac cggattacca tcaaggtatg cagtccagcc
     1741 tttatcgtaa tagatctcgg aaaacacaac gaaaccgtct tcagaactgt tcgatttata
     1801 gatgatctga ttgggcaaat aggactccac ttttacgaaa gccatcgaat cgaaaccggg
     1861 atcctgattt tttacttttt ccgcaaaccg gcgatcaacc agaactgaat cttcgatggc
     1921 ataattggta ttcctgaacg ttacagaatc aattgttttt ccatttcgtg caacctgtaa
     1981 tgtttctgcc ggttcgagat tggcaacagc aagtacttct tcatttgcgt tttccacaaa
     2041 acgaacatta cgtgcaaacc aagctgcacc ttttgctttt gggtttaatg ctgccggtat
     2101 cgaagaatca agaatcaggt attttgtgtt gagcatattc agaacatgag ttctttcgaa
     2161 taagcccgac atcatcaaca cagatggaat ttgattggca ggaacattgt tttgtgcaac
     2221 ctgcattaaa aatccatatg ccatgttatg ctctctgcgg attgcaaact caatgagctc
     2281 ctgataccgt ttcatttttg ctccgtggta tccgccaacc gatttatgga aataagaagt
     2341 gcgcgaagaa ttaaaaggat ctcctatttc aagcactctg taattgcttg cgaaattaag
     2401 tgcaggaaag gcaccatcaa caatttgggc caattccagg ttctgtcctt tttccacttt
     2461 tacgttcgac atttcctgct gaatctttgt cattagcgaa ggatcagcag tagcctcctg
     2521 ctgcagtacc gacatatccg cttcagttgg gtaatacgga taatcgcctt ttttctgcca
     2581 tgacacgtac tggccgtttt ctttttcatt attgagataa cggcgatcaa aactgtagag
     2641 atccgcaatg atcaatacac ctaatccggc aaccagcaaa ttgcgtgaaa acgatttcgt
     2701 aatgtaaaaa tagaggagta aaaaacctgc aaggataaat ccaaatgaac gcaaggcatc
     2761 agcgcgaaat acaccaaccc ggtaatcgac caattgcgat tgaagatcac ttacaatcgg
     2821 atttctttca ttagaaaatg cagtgagatc agattcgctg aggaacgaaa taaatgattg
     2881 tggaaccaaa accactaaga gtaaaaatcc acc
//
LOCUS       DAOMYG010000406         2903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_12694_length_2903_cov_7.872191, whole genome shotgun sequence.
ACCESSION   DAOMYG010000406 DAOMYG010000000
VERSION     DAOMYG010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12694_length_2903_cov_7.872191"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PK610_11715"
     CDS             complement(<1..183)
                     /locus_tag="PK610_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRJ36651.1"
                     /translation="MLPKKVETALNAQIEIEAESSQFYLAMASWAECNGLPGVAEFLY
                     AHSDEERMHMLKLVKYV"
     gene            complement(289..1392)
                     /locus_tag="PK610_11720"
     CDS             complement(289..1392)
                     /locus_tag="PK610_11720"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ36652.1"
                     /translation="MSDKKNIEELFRDKFSGFESDPGAGSWNAIQSRIPSPAAGTGLG
                     SGLFSAKILTIVSVVSVVAGLGIGYLIGGGDSATTKETDTEIAAKTDQVVEATIDSPE
                     EENILSSTVVIEEPVSMDAPEGPKQKVVIKEVPEPGSIAQSWLTPSDRKKPFVAKGSE
                     PVATSSNEKQNPAATQISSEEKKTNWVETKVDESLPVAVIGTSLTGGPAPLYVDFKSV
                     YAAQQYEWNFGDGKTSKEVSPVHKFEEPGNYQVTLVVKDAKGNSSTDQVTIQVKEGSV
                     LNLPNVISPNNDGYNDVFTCQECKNIASIYLVVSDRSGNTIYFETTDTNFAWDGRDRS
                     GKTVADGIYIVQFKAYDTYGKEYKKVFSLTVKQ"
     gene            complement(1398..1994)
                     /locus_tag="PK610_11725"
     CDS             complement(1398..1994)
                     /locus_tag="PK610_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRJ36653.1"
                     /translation="MYFATPCNLFAWFDVFIPERPMTDEQLIKECLSGRPSAQKTLYE
                     RFSRKMMGVCIRYSSSYEEAQDVLQDGFVKVFEKLGTYSGNGSFEGWVRRIMVNTALD
                     SWRKNKQEREMQDIDEVGYGLSSGEDIVSNITAEDLLAILQTIPSGYRMVFNLYVIEG
                     YSHKEIGEMLNITESTSKSQFSRARSYLMNILQKQNML"
     gene            2050..>2903
                     /locus_tag="PK610_11730"
     CDS             2050..>2903
                     /locus_tag="PK610_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HRJ36654.1"
                     /translation="MRRYPLFLLLVLAACSSPVKESTKPSTLPAGTWRLQLNLGEGLF
                     LPFCFTLSEENSVYTAEIINGSEKIRTEQFSVTDDSVFFRLPVFDSEFKMKRNGDHTL
                     EGTWFNYSKSPDYKIPAFAEYNKTERLTKDETKGEDASFKGKWKVTFSKGSEEQYMAV
                     GLFEQEKDIVTGTFITETGDYRYLEGRVNGNTMQLSCFDGSHAFYFTATLLDTSSIDG
                     MFYSGTHWKEPWVAVRDDAFELSDPESLTYIKEGYSGLEFSFPDIDNNTVSFPSAEYS
                     GKVTIVEL"
BASE COUNT          820 a          654 c          575 g          854 t
ORIGIN      
        1 tacatatttt accagcttta gcatgtgcat acgttcctca tcagaatgcg cataaagaaa
       61 ctcagcaacg ccgggtaagc cgttgcactc agcccaagaa gccattgcaa ggtaaaattg
      121 agaggactca gcttcaattt ctatttgtgc gtttagcgcc gtttcaactt tcttgggtag
      181 catattttta aaaatttttt tcaaaaatac ggcttttcct gtccaaagcg aaagaaaaaa
      241 ccccggaaat gaaatcccgg ggctctccaa agcaagcagt aatctccttt attgtttcac
      301 agtaagtgaa aacacttttt tgtattcctt tccataggta tcgtatgcct tgaactgcac
      361 aatgtaaatt ccatcagcaa ccgtcttgcc ggaccggtcg cgaccatccc atgcgaagtt
      421 ggtatctgtt gtttcaaaat agatggtgtt tcctgaacgg tctgaaacaa ccagataaat
      481 cgaagcaatg tttttgcact cctgacaagt aaagacatca ttatatccgt cgttattcgg
      541 acttattaca ttaggaagat taagaacaga cccttccttg acctgaatag taacctggtc
      601 ggtagagctg tttcctttag catcttttac tacaagcgta acctgataat tacccggctc
      661 ttcaaactta tgtaccggcg aaacttcttt cgatgttttt ccatcgccaa aattccattc
      721 atactgttgt gcagcatata cacttttgaa atccacatac aatggagcag gaccgcctgt
      781 taatgaagtg ccaataactg caacaggtaa tgattcatca acttttgttt ctacccaatt
      841 ggtctttttt tcctccgaag aaatttgtgt tgctgccgga ttctgttttt cattggagct
      901 tgttgcaaca ggctctgaac ctttggcaac aaatggtttt ttacgatcag agggtgtcaa
      961 ccagctttgc gcaatagaac cgggttccgg aacctcttta ataacaactt tttgtttcgg
     1021 accctcaggg gcgtccatag ataccggttc ctcaattaca actgttgagc taagtatgtt
     1081 ttcttcttcg ggtgagtcaa tagttgcttc aacaacctga tcggtttttg cggctatctc
     1141 tgtgtctgtt tccttagttg tagccgaatc accaccgcca atcaaatacc caattccaag
     1201 acctgctaca acagagacta cggatacaat cgtcaaaatt ttcgctgaaa aaagccccga
     1261 accaagacct gtaccggcag caggggaagg aatgcgcgac tgaatggcat tccaggaacc
     1321 agcacccgga tcggattcaa atccgctgaa cttgtcgcgg aataattctt ctatgttttt
     1381 tttgtcgctc acagcgttta taacatgttt tgtttctgca gaatgttcat taaataactt
     1441 cgtgctcttg aaaattgact cttggaagtg ctttccgtaa tattcagcat ttcaccgatc
     1501 tccttgtggc tataaccttc aataacataa agattaaaaa ccatccggta accggaaggt
     1561 attgtttgca atatggccag aagatcttca gcggtgatgt tcgaaacaat gtcttctccc
     1621 gaacttaaac cgtagccgac ctcatcaatg tcctgcatct cgcgctcctg cttatttttt
     1681 cgccaggaat cgagggcagt gttgaccatt attctgcgta cccaaccttc aaaagaacca
     1741 ttcccggaat atgtaccgag tttttcaaaa accttcacaa aaccatcctg caaaacgtcc
     1801 tgcgcctctt cataactact tgaatatctt atacaaacac ccatcatttt tcgtgaaaaa
     1861 cgctcataaa gcgtcttttg cgctgatggg cggcctgaca gacattcttt gattagttgc
     1921 tcatcggtca ttgggcgttc cggaataaag acatcaaacc atgcgaaaag gttgcatgga
     1981 gtggcaaaat acacaaagtt tttccctgat attggaattc tgggtaatat tgaggcctgt
     2041 agaaaacgaa tgagacgtta tccccttttc cttcttcttg tgctggcggc ttgttcgtca
     2101 cccgttaaag aatcaaccaa accatcgaca ttacccgccg gaacctggcg actgcaactc
     2161 aatttaggcg aggggctgtt ccttcctttt tgttttacac tcagcgaaga aaacagtgtt
     2221 tatacagccg aaattataaa tggaagcgaa aaaataagaa cagaacaatt ttctgtaacc
     2281 gacgactccg tttttttccg gctacctgtt ttcgatagcg aattcaagat gaaacgcaac
     2341 ggcgaccata cactggaggg gacttggttt aactattcaa aaagtcccga ctacaaaatt
     2401 cccgcttttg ccgaatacaa taaaaccgaa cgattaacca aagacgaaac gaaaggggaa
     2461 gacgcctctt ttaaagggaa gtggaaagtt acctttagca aaggaagtga agagcaatat
     2521 atggctgttg gattgtttga acaggaaaaa gatattgtta caggcacttt tattaccgaa
     2581 acaggagatt atcgttattt ggaaggacgt gtgaacggca ataccatgca attatcctgt
     2641 ttcgacggaa gtcacgcttt ttatttcacc gccacgcttt tggatacatc atccattgat
     2701 ggtatgttct attccggaac ccattggaaa gagccctggg tagctgttcg tgatgatgcg
     2761 tttgaacttt ccgatcccga aagtctaacc tacataaaag aagggtatag cggtttggaa
     2821 ttttcatttc ctgatatcga taataacacc gtttcatttc cttctgcaga gtattccgga
     2881 aaggttacga ttgtggagtt aat
//
LOCUS       DAOMYG010000407         2898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_12724_length_2898_cov_6.785086, whole genome shotgun sequence.
ACCESSION   DAOMYG010000407 DAOMYG010000000
VERSION     DAOMYG010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12724_length_2898_cov_6.785086"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /gene="era"
                     /locus_tag="PK610_11735"
     CDS             complement(<1..614)
                     /gene="era"
                     /locus_tag="PK610_11735"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRJ36655.1"
                     /translation="MAHKAGFVNIIGSPNVGKSTLMNALVGEKVSIINAKAQTTRHRI
                     MGIASGEDFQIVYSDTPGVLKPAYKLQEGMMSFVNQAFQDADVFLFVTDIHEDKPQVP
                     EVLERLKKQSVPVFVLVNKIDLSDQEKVGARLDYWKEQIPNAMVFGVSALHKYNLDVL
                     FDKILEKLPESPAFFDKEEYTDKSLRFLVSEIIREKILRLYDKE"
     gene            671..745
                     /locus_tag="PK610_11740"
     tRNA            671..745
                     /locus_tag="PK610_11740"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:703..705,aa:Met,seq:cat)
     gene            complement(1000..1272)
                     /locus_tag="PK610_11745"
     CDS             complement(1000..1272)
                     /locus_tag="PK610_11745"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRJ36656.1"
                     /translation="MKAFFVPYYVYILRSLKNGSFYKGSTDNIDRRLAEHNSLKEKST
                     SRFAPWELCLVIEKPNRSEAVILEKKLKNLNAEKTFAFINRYSEKM"
     gene            complement(1285..1356)
                     /locus_tag="PK610_11750"
     tRNA            complement(1285..1356)
                     /locus_tag="PK610_11750"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1321..1323),aa:Arg,seq:ccg)
     assembly_gap    1359..1458
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1692..2525
                     /locus_tag="PK610_11755"
     CDS             1692..2525
                     /locus_tag="PK610_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36657.1"
                     /translation="MITGQNEFVGVIKIITLGLKYQYAMIRIMSTLCLVLASITNLFA
                     QSDAELLSKAKKLQDERSHEEALKVYKQIYSKDSSNVEVVSNMSLCFSRSGALMTANS
                     ADQKKYYAEAKRLAKKSIAMNTQNANSHLAMAVALARENENASSKTKISNAKEIRSEC
                     DLVFKYDPDNATAFHIMGRWHRTFAGLNGFEKTMINTLYGGVPKGGTYADALEMFSKA
                     IKIEPSLSMHVYELAVTYYEMGQKENAKKFANKVMDYPVKSKDDEKTRKECEKLLAKL
                     K"
     gene            complement(2526..2753)
                     /locus_tag="PK610_11760"
     CDS             complement(2526..2753)
                     /locus_tag="PK610_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36658.1"
                     /translation="MNKKTKKRILIVLVAIAAIAIPLVIWMKQPAEITKQDEDKAVEI
                     ASDDELLNAIDAGVSGSNEVNSSDSEDTIKH"
BASE COUNT          882 a          557 c          541 g          818 t
ORIGIN      
        1 atttccttat cataaagacg taaaattttc tcacggataa tttccgaaac cagaaagcga
       61 agtgatttat cagtgtattc ttctttatcg aaaaacgcag ggctttccgg taacttttcg
      121 agtatcttat caaaaaggac atccagattg tatttatgca atgctgaaac tccgaatacc
      181 attgcattgg gaatttgttc tttccaatag tctaatcgcg caccaacttt ttcctgatcg
      241 ctcagatcaa ttttattcac cagcacaaaa acaggtaccg attgtttttt caaacgctcc
      301 aatacctcag gcacctgtgg cttgtcttca tgaatatcag ttacaaacaa gaatacatcc
      361 gcatcctgaa aagcctgatt aacaaagctc atcattcctt cctgaagttt gtatgcaggc
      421 ttcaatacac ctggtgtatc agaataaaca atttggaaat cttcaccact agcaataccc
      481 atgatccgat gacgggttgt ttgcgccttt gcattgataa tagacacttt ctccccaacc
      541 aatgcattca ttagtgtgga tttcccaaca tttggacttc caataatatt tacaaaccct
      601 gctttatggg ccatctacta tttttttaca aaggtatcaa tctatttcct atatttgcag
      661 tccaaaacaa tgcggggtag agcagaggta gctcgtcggg ctcataaccc gaaggtcatt
      721 ggttcgaatc cagtccccgc taccaacaaa acgaaaaagg ccacaggaaa ctgtggcctt
      781 tttcgttttg ttgccctaaa gcgatccgac aaaggaggat ctgctttagg ggacaatttc
      841 cattttattg atcaaatctt atgcatgaaa cgaggaaatg ctttcgttaa aatgtaaacg
      901 taacagggta taaaaaagct ttcattggaa agcttagtag ccccaaggga atcgaatgcc
      961 ggcgtttcgg tcggcatcca gaccgaagcg tctggacccc tacatttttt cagaatatct
     1021 gttaataaaa gcgaatgttt tttctgcgtt taggtttttg agtttttttt caagaattac
     1081 cgcctcggaa cgattaggtt tttcaatgac caaacacaat tcccatggag caaaccgact
     1141 tgttgatttt tcctttaatg aattgtgctc tgctaatctt cggtcaatat tatcagtaga
     1201 acctttgtaa aaagatccgt ttttcagact acgtaatatg taaacatagt aaggcacaaa
     1261 aaaagccttc attggaaagc ttagtagccc cgaagggaat cgaaccccta ccaagagttc
     1321 cggaaactct cgttctatcc attaaactac ggagccaann nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnag agttccggaa actctcgttc tatccattaa actacggagc
     1501 caaaatggag tgcaaaggta aaaaatgaaa ttcaggtgaa tttttgaatc gaaaggcatc
     1561 gctaaggtcc gtctatccga ccggcctgtt ccaacgtaat acgtatggaa gcatgtggac
     1621 cctaaaaaga attccggaaa tatctttttg tccagataac ctaattaccc tggcggacgg
     1681 aaggaaactt attgattact ggccaaaatg agttcgttgg tgtaatcaaa atcattacct
     1741 tagggcttaa atatcaatat gctatgatta gaatcatgtc aaccctatgc cttgtattgg
     1801 cttcaataac caatctattc gcccaatccg atgcggaatt gctttcgaaa gcaaagaagc
     1861 ttcaggatga acggagtcat gaagaagcct taaaagtgta taaacaaatc tattcgaagg
     1921 acagtagcaa tgttgaggtt gtgtcgaaca tgagtttatg ttttagccga tcgggtgcac
     1981 tgatgacagc caattctgcg gatcaaaaga agtattatgc agaagcaaaa cgtctggcga
     2041 aaaaatcgat tgcaatgaat acgcagaatg ctaattctca tttggcaatg gcagtggcgc
     2101 tggccagaga aaatgaaaat gcatcctcaa aaacaaaaat ttccaacgca aaagaaattc
     2161 gtagcgaatg tgatttggtg ttcaaatatg atccggataa tgcaaccgct tttcacatta
     2221 tggggcgctg gcacaggaca tttgcaggct tgaatggctt tgagaaaaca atgatcaaca
     2281 cactttatgg cggtgttcca aaaggaggca cctatgcaga tgcgcttgag atgtttagta
     2341 aggccattaa aatcgaacct tcactctcaa tgcatgtcta tgaacttgct gtgacctatt
     2401 atgaaatggg tcaaaaagag aatgcaaaaa agtttgcaaa caaggttatg gattatcctg
     2461 taaaatcaaa ggatgatgag aagacccgca aagaatgcga aaaacttctt gctaagttga
     2521 aataattaat gtttgatagt gtcttctgaa tcggaagaat tcacttcatt actccctgaa
     2581 accccagcgt caattgcgtt aagtaattcg tcatcacttg caatttctac cgctttgtct
     2641 tcatcctgtt tggtgatttc agcaggctgt ttcatccaaa ttacaagcgg aattgcaatt
     2701 gcagcaattg caactaaaac aattagaatt ctctttttgg ttttcttatt catgaccatg
     2761 ggttattggt ttcaacaaga tagggaattt tcgaaatgat gataatttca tttccgggtt
     2821 taagtcgcat aaaaacacaa ttaactatta acattcaagg gtttatttgt agaagcatgt
     2881 aaatagttaa tagttaat
//
LOCUS       DAOMYG010000408         2894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_12749_length_2894_cov_8.976048, whole genome shotgun sequence.
ACCESSION   DAOMYG010000408 DAOMYG010000000
VERSION     DAOMYG010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12749_length_2894_cov_8.976048"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /gene="rpoN"
                     /locus_tag="PK610_11765"
     CDS             complement(<1..1260)
                     /gene="rpoN"
                     /locus_tag="PK610_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRJ36659.1"
                     /translation="MLKQSLHQKLLQKLSPQQIQLMKLLQVPTAELEQRIKEEIEVNP
                     ALEEGQSEDERERDEEFGDDEYGQDENSDERNDFDINDYLDDDMPAYKTQVNNRGADD
                     EDRSMPISSGDSFQENLLAQISLRIINERQKQIAIYIIGNLDDSGYLRRETENIVDDL
                     AFSMNLSVTQEEIEKVLGIIQDLDPPGIGARTLQECLLIQLRRKENDSIGIENAIDII
                     EHQFDEFTRKHYDKIQKKLSLSDENLKFAIEEIIHLNPKPGNSAKDGSRVGMQHIIPD
                     FLLSEENGKLELSLNSRNAPQLRISKDYEEMLRAYAENKNDKKNKDAVTFVRQKIDAA
                     KWFIDAIRQRQQTLLMVMNAIMEYQREYFISGDETKLRPMILKDIADVVGMDISTVSR
                     VANSKYVQTHFGTFLLKSFFSESLSTDS"
     gene            complement(1344..2777)
                     /gene="asnS"
                     /locus_tag="PK610_11770"
     CDS             complement(1344..2777)
                     /gene="asnS"
                     /locus_tag="PK610_11770"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRJ36660.1"
                     /translation="MKRIKIRQVLQTQPQEQIITIKGWVRTFRNNQFLSVNDGSTLNN
                     LQAVIDFENTPESELKRLTTGAAVSITGKLVASQGKGQSVELIAQSFEILGDSDAEKF
                     PLQPKKHSLEFLREIAHLRFRTNTFSAVFRVRHALAFSIHQFFHERGFVYLHTPIITA
                     SDAEGAGEMFRISTLDPKNPPLNDAGEIDYSQDFFGRQANLTVSGQLEAELGAMALSE
                     VYTFGPTFRAENSNTTRHLAEFWMIEPEVAFNDLNDNMDLAEDLLKYVIRFALDNCAE
                     DIEFLRTRLLEEEKQKPQNERSEMDLVQKLQFCLDNSFERLTYTEAITILMESPQNKK
                     KKFQYPVTGWGTDLQSEHERYLVEKHFKKPVILTDYPKHIKAFYMRMNEDNNTVRAMD
                     ILFPGIGEIVGGSQREERLDILEARMKEMHIPTEEMYWYLDTRRFGTAPHAGFGLGFE
                     RLVLFVTGMTNIRDVIAFPRSPKNCEF"
BASE COUNT          730 a          609 c          632 g          923 t
ORIGIN      
        1 gctatcggta gaaagggatt cgctgaaaaa ggatttaagt aaaaacgttc cgaaatgcgt
       61 ttgcacatat ttgctgttag ccacacggga aacagttgag atatccattc caactacgtc
      121 ggcaatatcc ttaaggatca tcgggcgcaa cttggtttca tcaccggaaa taaagtattc
      181 acgctggtat tccataatgg cattcattac catcagcagg gtttgctgac gttgacgaat
      241 tgcatcgatg aaccattttg cagcgtctat tttctgtcgt acaaaagtaa cagcgtcttt
      301 gttcttttta tcgtttttgt tttcggcata ggcacgcaac atctcttcgt aatctttcga
      361 aatgcgcagc tgaggtgcgt tacgtgaatt gagactgagt tccaactttc cgttttcttc
      421 gctcaatagg aaatcaggaa taatgtgctg cattcctact cttgaaccat ctttagccga
      481 attaccgggt ttgggattaa gatggataat ttcttcgatg gcgaatttta ggttttcgtc
      541 actaagcgat aattttttct gaatcttatc gtagtgtttt cgggtgaact catcaaactg
      601 gtgttcgatg atatcaatgg cgttttcaat gccgatactg tcgttttctt ttcttcgtag
      661 ttgaataagc agacattcct gcagtgtacg cgctccaatc ccggggggat caaggtcctg
      721 tataatgccc aacacttttt caatctcttc ctgcgttaca gaaagattca tggaaaaggc
      781 aagatcgtcg acaatgtttt ccgtttctct tctcaggtat ccggaatcgt cgagattacc
      841 gatgatgtag atggcaatct gtttctgacg ttcgttgatg attcgcaaac tgatctgcgc
      901 aagaagattt tcctgaaagg aatctcctga tgaaataggc atcgaacgat cttcatcatc
      961 cgcgccccgg ttgttgacct gtgttttgta cgcaggcata tcatcgtcga gatagtcatt
     1021 aatgtcgaaa tcattacgtt catccgaatt ttcgtcctga ccgtattcat cgtcgccaaa
     1081 ctcttcatcg cgttcacgtt catcttccga ctgaccttct tccaacgctg gatttacttc
     1141 tatttcttct ttaatacgtt gttccaactc agcagtaggc acctgcaata gcttcatcag
     1201 ctgaatctgt tgaggagaca gtttttgaag taatttttgg tgtagcgatt gcttaagcat
     1261 ggtaaggcga aaatacggaa aataattggc tcaacagagt aaagggatct gtgtattatt
     1321 cgttcgcctt cagttggttt cgtttagaac tcacaattct ttggcgacct tggaaaggcg
     1381 atgacgtcgc ggatattggt cattccggta acgaacaaaa ccagccgttc aaaaccaaga
     1441 ccgaatcccg catgcggtgc agttccaaaa cgacgggtgt cgaggtacca gtacatttct
     1501 tcggttggaa tatgcatttc tttcatgcgt gcttccagga tatcgaggcg ttcttcacgc
     1561 tgcgatccgc ccacaatctc tccaatgccc gggaaaagaa tatccattgc acgaaccgtg
     1621 ttgttgtctt cgttcatgcg catgtaaaag gccttgatat gtttggggta atcagtgaga
     1681 atgacgggtt ttttgaaatg tttttctacg agataacgtt cgtgttcgct ctgtagatcc
     1741 gttccccatc ctgtaaccgg atactggaat tttttctttt tattttgcgg tgattccatc
     1801 agaatggtaa tcgcttctgt ataggtgagg cgctcgaaac tgttgtcaag acaaaactgc
     1861 aatttctgaa cgagatccat ttcagatcgt tcattctgcg gtttttgttt ttcttcttcc
     1921 agtagtcgtg tgcgaaggaa ttcaatatcc tctgcacagt tgtcgagggc aaaacggatc
     1981 acatatttta aaagatcttc tgcaagatcc atgttatcgt taagatcatt aaatgcaact
     2041 tccggttcga tcatccagaa ttctgcaagg tgacgcgtag tgttcgagtt ttcggcacgg
     2101 aaggttggac cgaatgtata cacttcactc agtgccatcg ctcctaattc tgcttcgagc
     2161 tggcccgata cggtaaggtt ggcttgtctt ccgaaaaagt cttgcgagta atctatttct
     2221 cctgcatcat ttaatggtgg attttttgga tcgagcgttg atatacgaaa catctctccg
     2281 gctccttctg catcggatgc ggtaataatg ggtgtatgca gatacacaaa acctctttca
     2341 tgaaaaaatt gatgaataga aaaagccaaa gcatgacgca cacggaatac tgcactaaac
     2401 gtattggtgc ggaaacgtaa atgcgcaatc tcgcgcagaa attccagtga gtgttttttg
     2461 ggttgcaggg gaaatttttc tgcatcactg tcgcctagaa tttcaaaaga ttgcgcaatg
     2521 agttcaaccg actgtccttt tccttgtgag gcaacaagtt tacctgtaat ggaaacagca
     2581 gctccggttg ttaaacgctt gagttctgat tcaggtgtgt tttcgaaatc aattactgct
     2641 tgtaaattat tcaatgtgga tccgtcgtta accgacaaaa actgattatt acgaaatgtt
     2701 cgaacccaac ctttaatggt aatgatttgt tcctgtggtt gtgtttggag tacctggcga
     2761 atctttatgc gtttcacggc tgttttgttt ttttgtcctg caaaggaaag aaattttcaa
     2821 gcagcccttt tttatggacc tggatacaat atcggaccgt ctaaaccgtc tgtttccgta
     2881 tgttgcaaat ccat
//
LOCUS       DAOMYG010000409         2886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_12804_length_2886_cov_9.976687, whole genome shotgun sequence.
ACCESSION   DAOMYG010000409 DAOMYG010000000
VERSION     DAOMYG010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12804_length_2886_cov_9.976687"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PK610_11775"
     CDS             <1..384
                     /locus_tag="PK610_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ36661.1"
                     /translation="TPRKGDKEYIRNKVSLTFYDPKHATDEIVDECYGILSDKTKLIR
                     ILSLAKSAIRHNMAADLPSLTLPTCLVWGKQDTITPPEVAEEFKRLMPNAELFWIDKC
                     GHAPMMEHPEEFNRIVKDWLGKFGI"
     gene            386..982
                     /gene="yihA"
                     /locus_tag="PK610_11780"
     CDS             386..982
                     /gene="yihA"
                     /locus_tag="PK610_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRJ36662.1"
                     /translation="MEIRSAEFVSSYVKLDQLPRPEKPEYAFIGRSNVGKSSLINMLT
                     GRKYLAKISSTPGKTQTINHFIINNEWYLVDLPGFGYAKTSKTLRALWKGFTFEYLKQ
                     RENLLCVCVLLDSRLEPQKIDLEFMEWLGKNGIPFVMVFTKADKNTKRELVKNLENYQ
                     RKMLETWEFLPQIFVTSAEKQTGKEDLLAFFDEVNERF"
     gene            complement(985..1320)
                     /gene="gldC"
                     /locus_tag="PK610_11785"
     CDS             complement(985..1320)
                     /gene="gldC"
                     /locus_tag="PK610_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013187072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HRJ36663.1"
                     /translation="MSAKTTEINITVSLDENHIPVDMKWTATDTGMENSPAKALMLAL
                     WDEKEKNTLRMDLWTKEMTTDEMKQFFHQTLLTMADTLERATGEEKMAGDMRDFCFYF
                     AEKLKLLEQ"
     gene            complement(1317..2264)
                     /locus_tag="PK610_11790"
     CDS             complement(1317..2264)
                     /locus_tag="PK610_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36664.1"
                     /translation="MVDVSQIEASVTIERMEKQLFDARPGELERLNKDFLERYGELWE
                     VYVSVMLRSGSPKDSMIVVSLDRFISDPTMIQVYQDISATYPDLDWLKEDLEQAFRYL
                     RYYYPDAAIPRVVTYNSVFNYGVSTFKDQIGLGLDMYLGPENRTVKKISPEALPQFLK
                     EKMIKEYMVVDIMRGWIEANYLDAFKGQDFLSRMIHEGKILYAMDALLPADPDHIKIR
                     YTPEQFGWCLQNEKNIWRDIVDKKLLYTTDQMEIRKFCDESPFTSGLPQNSPGRVGNW
                     LGWMIVRAYMKDHPELKLPDLLDEKNERKILKSYKPPKE"
     gene            2391..>2886
                     /locus_tag="PK610_11795"
     CDS             2391..>2886
                     /locus_tag="PK610_11795"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRJ36665.1"
                     /translation="MKKLTGIVLGLFLGTALMAQDGAGNEQNFRFGLNAMPSINFYKP
                     ADIKRFESNGAVMKFAWGLSLDFKLGGTAWLSTGLHVAYDGGKIGFKDTVVSFIKDNE
                     FSTPDAFVQGAGVGFTQTRVEERKYSATYVTLPLNIKMKTKEIGYLNYYGLFGVSAGI
                     KVKSR"
BASE COUNT          877 a          643 c          579 g          787 t
ORIGIN      
        1 actccaagaa aaggcgacaa ggaatatatc cgaaacaaag tatcccttac gttttacgac
       61 cccaagcacg caacagacga aattgttgat gagtgctacg ggatactttc ggacaagacg
      121 aaactgatca gaattttatc gctagcaaaa tctgcgattc gccacaatat ggcggcagac
      181 ttacccagct taacgcttcc cacttgcctg gtgtggggta aacaagacac cattactccg
      241 cctgaggtag cagaagagtt taaacgtctg atgccaaacg cagaactatt ctggatcgat
      301 aaatgtggtc atgccccaat gatggagcac cctgaagaat tcaacagaat tgtgaaagac
      361 tggctgggga aattcggaat ctgaagtgga aatccgcagc gcagaatttg taagcagcta
      421 cgtaaagctc gaccagcttc cacgacctga aaagccggaa tatgctttta tcggaaggtc
      481 gaacgtggga aaaagttccc tcatcaatat gcttacgggg agaaaatatc tagctaaaat
      541 ttcttcaaca cccggaaaaa cgcaaaccat taaccacttc atcattaaca acgaatggta
      601 cctggtagat cttcccggtt ttggttatgc aaaaacatca aaaacgttgc gggcactttg
      661 gaaaggattc acttttgaat acctgaaaca aagagagaat ctgctatgtg tttgtgttct
      721 tctcgactcc agacttgaac cccagaaaat cgatttggaa ttcatggagt ggctcggaaa
      781 aaatggaatt ccatttgtga tggtatttac caaggccgac aaaaacacca agagggaact
      841 tgtaaaaaat cttgaaaatt accagcgaaa aatgctcgag acttgggagt ttcttcctca
      901 gatatttgtc acctcagccg agaaacaaac gggcaaggaa gatctgcttg cattctttga
      961 tgaagtgaac gagcgttttt agaattactg ctccagcagt tttagctttt ctgcaaaata
     1021 aaaacagaaa tcacgcatat cacccgccat cttttcttca ccggtagctc gctcaagcgt
     1081 gtctgccatt gttaaaagtg tttggtgaaa aaactgtttc atttcatcgg tagtcatctc
     1141 ctttgtccag agatccattc gtagagtatt cttttccttt tcgtcccaaa gcgcgagcat
     1201 taatgctttt gcaggactat tctccatccc tgtatcggta gcggtccatt tcatatccac
     1261 aggaatatga ttttcatcca gactaacagt aatattgatt tcagttgttt tggcactcat
     1321 tcctttggcg gtttatacga tttcaaaatt tttctttcat ttttttcatc cagtaaatcc
     1381 ggaagtttta attcaggatg atccttcatg taagcacgaa caatcatcca gcccaaccag
     1441 ttccctaccc ggccgggaga attttgcggg agtccgcttg taaagggcga ttcatcacaa
     1501 aactttcgaa tctccatctg atcggtggta tacaacaatt ttttatcgac tatgtcccgc
     1561 cagatatttt tctcattctg caaacaccaa ccaaactgtt cgggagtata tcttatctta
     1621 atgtgatcag gatcggcagg aagcaaagca tccatagcat atagaatctt tccttcatgg
     1681 atcatccgtg aaagaaaatc ctggccttta aaagcatcca aataattggc ttcaatccat
     1741 ccgcgcatga tgtcgaccac catatattcc ttgatcattt tctctttcaa gaattgcggc
     1801 aaggcttcag gagagatttt ttttacagtc cggttttccg gtcccagata catatccaat
     1861 cccaacccaa tctgatcctt gaatgtggag acaccgtaat tgaaaacaga attgtaggta
     1921 acgacacgag gaatggcggc atcgggataa taatacctca aatagcgaaa tgcctgctcc
     1981 aagtcttctt ttaaccaatc aaggtcggga taagttgcag agatatcctg atagacctga
     2041 atcatggttg gatcactgat aaaccgatcc agagaaacaa cgatcatgga atcctttggg
     2101 gagcccgaac gcaacattac actcacatag acctcccaaa gttcaccgta tcgctccaga
     2161 aaatccttat tcaaccgctc caattcgccg ggacgagcat caaatagttg cttttccatt
     2221 cgttcgatgg taacactggc ttcaatttgg ctaacatcaa ccattagtgg atttttctcc
     2281 ccacaagagg ataaaacaac catcgaaatg aatacacaaa aaaacctttt caccttatct
     2341 gttgaaatat gagaattgcc tattattgca ctgaaatcaa cgctaaaact atgaaaaaac
     2401 ttactggaat cgtgttaggc ctgtttttag gcacagctct gatggctcag gacggtgcag
     2461 gaaatgagca aaatttccgc ttcggtctga atgcaatgcc ttcaatcaac ttctacaagc
     2521 ctgcagatat caagcgcttc gaaagcaatg gtgccgttat gaagttcgcc tggggcttaa
     2581 gtctcgactt caaacttggc ggaactgctt ggttatcgac cggattacac gtggcttacg
     2641 atgggggaaa aattggcttt aaggatactg ttgtttcttt tataaaagac aatgagttca
     2701 gtacccctga tgcatttgta caaggtgccg gtgtaggatt tacacaaaca cgcgttgaag
     2761 aaagaaaata cagcgcaacg tatgttaccc ttcccctgaa tattaaaatg aaaaccaaag
     2821 agatcggata ccttaactat tacggtttat tcggtgtttc tgccggaatc aaagtaaaat
     2881 caagag
//
LOCUS       DAOMYG010000410         2878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_12861_length_2878_cov_7.148778, whole genome shotgun sequence.
ACCESSION   DAOMYG010000410 DAOMYG010000000
VERSION     DAOMYG010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12861_length_2878_cov_7.148778"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /gene="rlmD"
                     /locus_tag="PK610_11800"
     CDS             complement(<1..1145)
                     /gene="rlmD"
                     /locus_tag="PK610_11800"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRJ36666.1"
                     /translation="MGSAKKAMIGKIVENLEVIDAAAEGKSIARWDERVVFVNGGVPG
                     DIVDVKIIQQKRRFLEGVPVHFHKESAFRTEPFCAHFGNCGGCKWQNLAYEKQLHYKE
                     KQVRDNFERIGHLEFPSIRPILGAPKTSRYRNKLEFTFTDREWLTFEQVREGTTTIGP
                     ALGFHVPGKFDRVMRIDECHLMDEPANSIRNWLFQYALEKEISFFNLRSQEGLLRNLV
                     IRNTQAGEVMVTVVFAYDVESERKEILDALSEKFPEITSLFYVINTKRNDSLYDQDMI
                     LYKGLPHMTETMEGLKFIIGPKSFYQTNSEQAYHLYAVARELAGLKGDEIVYDLYTGT
                     GTIANFVASKAKKVIGVEYVDAAIEDAKKNSAANGIKNTDFFAGDMK"
     gene            1214..2500
                     /gene="rocD"
                     /locus_tag="PK610_11805"
     CDS             1214..2500
                     /gene="rocD"
                     /locus_tag="PK610_11805"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013187461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HRJ36667.1"
                     /translation="MMTMTHSGKTAEELMSLEDKYGAHNYHPLPVVLDRGEGVFLWDV
                     NGKRYYDFLSAYSAVNQGHCHPRIIGAMTAQAGKLTLTSRAFYNNMLGVYEKFVTGYF
                     GYDKVLPMNTGAEAVETAIKICRKWAYEKKGVAEDQAVIVVCKQNFHGRTTTIVSFSS
                     DDDSRKNFGPFPSGFVAIPYNDLSSLEKMLENPNVAGFLVEPIQGEAGVNVPAEDYLR
                     QAASMCKAKKVLFIADEIQTGIARTGSLLATCGNCSCEKKCERQPSTYFKADMLILGK
                     ALAGGVYPVSAVLADDEVMMVIKPGQHGSTFGGNPVACAVAIESLNVVNDESLIKNAR
                     ELGKVFRSEMEKLIAESPLIKLVRGKGLLNAIVINDTPEGKTAWNLCLKLAENGLLAK
                     PTHGNIIRFAPPLVMTEEQLMDCVSIIRKTVLEYKV"
     gene            complement(2589..>2878)
                     /locus_tag="PK610_11810"
     CDS             complement(2589..>2878)
                     /locus_tag="PK610_11810"
                     /inference="COORDINATES: protein motif:HMM:NF020691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leukotriene A4 hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HRJ36668.1"
                     /translation="DRDNSFGFTNSGNAEIACLWFQIAINSGYTEANPKIEAFLTSVG
                     RRKFLTPLYKAMIQIPEGKKLANKIYEKARPGYHFVATSTIDRIVGYQEKK"
BASE COUNT          773 a          608 c          619 g          878 t
ORIGIN      
        1 tccttcatgt caccggcaaa aaaatcagtg ttttttattc cgtttgctgc tgaattcttt
       61 tttgcgtctt caatcgctgc atccacatat tcaactccaa ttactttttt agctttggaa
      121 gcaacaaaat tggcaatggt accggtaccg gtgtataggt catacacaat ttcatcccct
      181 tttaatcctg caagctctct ggcaacagcg tagaggtgat aggcttgttc cgaattggtc
      241 tggtagaacg attttggtcc gattatgaat tttaatcctt ccattgtttc tgtcatatgt
      301 ggtaatcctt tgtaaaggat catatcctga tcatacaaac tatcgttacg cttggtattg
      361 atcacataaa acaatgaagt gatctccgga aatttttcgg atagtgcatc taaaatctct
      421 tttctttcag attctacatc gtatgcaaaa actacagtta ccatgacttc tcctgcctgg
      481 gtgttgcgta tgacaaggtt gcgcaataat ccttcctgcg aacggagatt gaaaaaactg
      541 atttcctttt caagcgcata ttggaataac caattgcgaa tggaattggc aggctcatcc
      601 atcaggtgac actcatcgat gcgcattacg cgatcaaact tacctggcac atgaaaaccg
      661 agtgccggtc cgatagtcgt agttccttca cgaacctgct caaacgtaag ccattcgcgg
      721 tcggtaaagg tgaattcgag tttattccga tacctggatg ttttcggtgc gccgagaata
      781 ggacgaatag aaggaaattc aagatgaccg attcgttcga aattatcgcg aacctgtttt
      841 tccttgtaat gcaattgttt ttcgtaggcg aggttttgcc atttacaacc tccacaattt
      901 ccaaaatgtg cgcagaatgg ttcggtgcga aatgcagatt ctttgtgaaa gtgaacagga
      961 acaccctcaa gaaagcgacg tttttgctga atgattttaa catctacaat gtcacccgga
     1021 accccgccat tcacaaatac cactcgctcg tcccaacgcg caatggattt tccttccgct
     1081 gcagcatcaa tgacttccag attctcaacg attttgccaa tcatggcttt ttttgcactt
     1141 cccatagagg ggtaaaatta gctatatttg tgtgttgaac agtttttatt gactgtgatt
     1201 taattgaaag acgatgatga ccatgaccca ctccggaaaa acagctgaag aattgatgtc
     1261 cctcgaggac aaatacggcg ctcacaatta ccatcccctt cctgttgtat tggatcgcgg
     1321 tgaaggcgtt tttctttggg atgtgaacgg aaaacgttat tatgattttc tatcagccta
     1381 ttccgccgta aaccaaggac attgtcatcc gcgcattata ggtgcaatga ctgcacaggc
     1441 cggaaagctc actttgactt cgcgtgcttt ttataacaac atgctgggag tttatgagaa
     1501 gtttgtaacc ggatattttg gttatgataa agtgcttccg atgaacactg gtgcagaagc
     1561 ggttgagaca gcgataaaaa tttgccgtaa atgggcttat gagaagaaag gtgttgcaga
     1621 agatcaggct gtgattgtag tgtgtaaaca gaattttcat ggtcgcacta ctaccatcgt
     1681 ttcattttcg agcgatgatg attcacgcaa aaattttggt ccatttcctt ccggatttgt
     1741 tgccattcct tataacgatt tgtcgtcgct tgaaaaaatg cttgagaacc caaacgttgc
     1801 aggttttctt gttgaaccta ttcaaggtga agcaggcgta aatgttcctg cagaggatta
     1861 tctccgtcag gcagcatcaa tgtgtaaagc gaaaaaggta ttgtttatcg cagatgagat
     1921 ccaaaccgga attgcacgta ctggttcgct gttggctacc tgcggaaatt gttcctgcga
     1981 aaaaaaatgt gaacgtcagc cttcaaccta tttcaaagcc gacatgttga ttctcgggaa
     2041 agccctcgcc ggtggcgtat atccggtttc ggcagtgctt gctgatgatg aggtaatgat
     2101 ggttattaaa cccggtcaac acggttcaac ctttggcgga aatcctgttg cctgcgccgt
     2161 tgccattgaa tcgttgaatg tggtaaatga tgaatcccta ataaaaaatg caagagaact
     2221 cggtaaggtc ttccgttcag aaatggaaaa actgattgca gaatcaccct tgatcaaatt
     2281 ggtgcgtgga aaaggcctgc ttaacgcgat tgtaattaat gatactcctg aaggtaagac
     2341 agcctggaat ttgtgtctta aacttgcgga aaacggcctt cttgccaaac ctactcatgg
     2401 aaatatcatt cgttttgctc ctcctttggt aatgacggaa gaacaattga tggattgcgt
     2461 ctctatcatc cgcaaaacgg tactcgaata caaagtttga taaagacgct actttattta
     2521 aacaaaaaag tccggtatat accggacttt ttcgtgcttt acctggtgtt atttccctta
     2581 tgggatcttt atttcttttc ctgataacct acaatacgat cgatggtgct tgttgccacg
     2641 aaatggtaac ccggacgcgc tttctcgtaa attttgttgg ccagtttctt tccttcagga
     2701 atttggatca tggctttgta aagtggtgtc aaaaactttc tgcggcctac actggtcagg
     2761 aatgcttcga tctttggatt ggcttcagtg tatccgctgt taattgcgat ctgaaaccat
     2821 aaacatgcaa tctctgcatt accggaattg gtaaacccaa agctgttatc ccggtctt
//
LOCUS       DAOMYG010000411         2851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_13057_length_2851_cov_7.526466, whole genome shotgun sequence.
ACCESSION   DAOMYG010000411 DAOMYG010000000
VERSION     DAOMYG010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13057_length_2851_cov_7.526466"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1564
                     /locus_tag="PK610_11815"
     CDS             <1..1564
                     /locus_tag="PK610_11815"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRJ36669.1"
                     /translation="FSLTFCLLLAVNCPDTKAQTADNWFFSFAAIHEIDIQFYESNFW
                     DSLTHYKSIDDQTGIEHYLAATIRVDTLLIDSVGVRLRGNSSYGHPGNKKPIQLDFNE
                     FVSGQKLDGLKKLNLNNSFLDPTQIREKLFLDVLNNMGLPSPRSTYVRVYFNGTYIGL
                     YKGIETVNKEFLDRRFGNSNGNLYRCEPDMPLTWEGSTQSAYYDNCELKTNETANDWS
                     DLIQFLNVLNNNPITNMPSALPQVFNMQDYIKAWAANNVFGNLDSYMYLAHNYYLYQN
                     TSTQKMEWVTWDVSLAFGVYAILVVPNSVDFDIRYLPQNARTSRPLNYFILENSILEK
                     MYFNAVCDIFCNEFDPVKLYPKIDSIAALIRSSVYAEPVTNQMFSPQQFEDNLERTQV
                     NYQLIGRIPGLKQFIAERRRNIEKQFCGYNWSCSKGERIPKTDEGFMQIYPNPVMEKV
                     TIEIAAEEESVSTSIRISDLSGRVIARYYPDYNTSNLNFQMDVSNLAQGVYVVSLASG
                     CRNATGKFVVVK"
     gene            complement(1651..2658)
                     /locus_tag="PK610_11820"
     CDS             complement(1651..2658)
                     /locus_tag="PK610_11820"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRJ36670.1"
                     /translation="MWHTGVISQGNTGTCWCFSTTSYYETEIKRLSGKEIKLSEMYTV
                     YWEYVERARYFVRNRGTMALGEGSQTMAVAKNMKKYGIVPASVYTGMKSGQKFHTHAK
                     MYEELEAYLKGVKVRGEWNEEAVVATVRSILDFHMGAVPSKFSFEGKEYTPGDFLKNV
                     TKLNPDDYVDFMSLMTAPYWEKALYDVPDNWVRSKDFINVPLDVFMDIIKSSIKAGYG
                     ISIGGDVSEAGFHSWSQVAVVPTFDIPSEYIDESARAFRFLNNTTTDDHAMHLVGYKE
                     VNGKTWFLIKDSGSGSRNCGEGCKSFGYYFFHEDYIKLKMMTFTIHKDAVKEVLKKVK
                     A"
BASE COUNT          833 a          614 c          557 g          847 t
ORIGIN      
        1 tttcagttta actttttgcc tacttcttgc agttaattgt ccggatacaa aggcacaaac
       61 tgcggataac tggttttttt ctttcgctgc catccacgaa atcgatatcc agttttacga
      121 gagcaatttt tgggattcac tgacgcatta caaaagcatc gacgaccaaa ccggcattga
      181 acattacctg gcagccacca ttcgtgtaga cactcttttg atcgactcgg taggcgtgcg
      241 tctccggggg aattcgagtt atggtcatcc gggaaataaa aaacccattc agctcgattt
      301 taatgaattc gtcagcggac aaaaactcga cggactgaaa aagttaaatc tcaacaacag
      361 ctttcttgat ccgactcaaa tccgcgaaaa attgtttctg gacgtactga ataacatggg
      421 acttccctca ccgcgttcga cctatgttcg ggtgtatttt aacggaactt acatagggtt
      481 gtacaaaggc attgaaaccg taaacaaaga atttctcgat cgacgatttg gtaacagcaa
      541 tggtaatctg taccgttgcg aacctgatat gcccctcacc tgggaaggaa gtacacagtc
      601 tgcttattat gataattgcg aattgaaaac aaacgaaact gcaaacgatt ggagtgattt
      661 gattcaattt ttaaatgtcc tgaacaacaa ccctattaca aacatgcctt ctgcattgcc
      721 acaggttttt aatatgcagg attacatcaa agcatgggct gccaacaatg tgtttggcaa
      781 tctggattct tacatgtacc tggcccataa ttattacctc taccagaata catccacaca
      841 aaaaatggaa tgggttacat gggatgttag ccttgctttt ggtgtttatg caattcttgt
      901 tgtcccgaat tcggtagatt ttgatatccg atatttacca cagaatgcga gaacaagtcg
      961 tccgcttaat tattttatac ttgaaaacag catacttgaa aaaatgtatt tcaatgcagt
     1021 atgcgacatt ttctgtaatg agtttgatcc tgtaaaattg tatccgaaga tcgatagtat
     1081 tgcagcgttg atccgctcct ctgtttatgc agaaccggtt accaaccaga tgttcagtcc
     1141 gcagcaattc gaagataatc tggaaagaac tcaagttaat taccagctca ttggaagaat
     1201 tcccggatta aaacaattta ttgctgaacg ccgacgcaat attgaaaaac aattttgtgg
     1261 atacaattgg agttgcagca aaggagagcg aattccaaaa acagatgaag gatttatgca
     1321 gatctatccg aatccggtaa tggaaaaagt aactattgag attgctgccg aagaggaaag
     1381 cgtgagtaca agcatccgta tttccgatct cagcggaaga gtaattgcaa ggtactatcc
     1441 ggattacaat acctccaatc ttaattttca aatggacgta agcaatctgg cacaaggagt
     1501 gtatgtcgtt tctctcgcat caggatgtcg aaatgcaacc ggaaaatttg tggtggttaa
     1561 ataggctttg tggcaaaagg aatacactat taataccaaa aaaatccccc gtcaaaactg
     1621 acgagggatt taaaaaatgg aaggcgaaaa tcaggctttc acctttttca aaacttcctt
     1681 cacagcgtct ttgtgaatag tgaatgtcat catcttgagt ttgatgtaat cttcatggaa
     1741 gaaataatac ccgaaagatt tgcatccctc accgcagttg cgtgatccgg aaccgctgtc
     1801 cttaatcagg aaccatgtct tgccgttgac ttctttatac cccaccaaat gcatggcatg
     1861 atcgtcggtt gtagtattat tcaaaaatcg aaatgcgcgt gcactttcat caatgtattc
     1921 gctcggaata tcgaaagtag gaacaacagc aacctgactc cagctgtgga atcctgcttc
     1981 agacacatca ccaccaatgc tgattccgta tcctgccttg atggatgatt tgatgatatc
     2041 cataaaaaca tcaagcggaa cattaataaa atctttgctg cgaacccagt tatcgggaac
     2101 atcatacaat gctttctccc aatatggcgc ggtcatgagc gacatgaaat caacatagtc
     2161 gtcgggattt aatttggtca catttttcag gaaatcaccc ggtgtgtatt cctttccttc
     2221 aaaactgaat ttggaaggca cggcacccat atgaaaatcg agaatggatc tcactgttgc
     2281 aactactgct tcttcgttcc attcgccacg tactttaaca cctttcagat aagcttcaag
     2341 ctcctcgtac attttagcat gagtatggaa tttctgaccc gatttcattc ctgtatacac
     2401 cgatgcagga acaattccgt acttcttcat attcttcgca actgccattg tctgcgatcc
     2461 ttctccgagc gccatggttc cgcgattgcg aacaaaataa cgtgcacgtt ccacatattc
     2521 ccaatacacg gtgtacattt ccgaaagttt gatctctttt ccggaaagac gtttgatctc
     2581 tgtttcataa taggaagtag tactgaaaca ccaacacgtt cctgtatttc cctgagagat
     2641 tactccggta tgccacactt tcgtgtatga ttccggatct gtcgggtagt ttttattgct
     2701 gacatcaact ttaaaaactt tgtttggtgc ttcttctttt ttggtctcct caaattcctg
     2761 aattcctttc agaatactat tctgataata cccaggcttt gtttcaataa aagtgcttca
     2821 ggttgtaagc tttctttttg tggtgttgta g
//
LOCUS       DAOMYG010000412         2846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_13084_length_2846_cov_9.526335, whole genome shotgun sequence.
ACCESSION   DAOMYG010000412 DAOMYG010000000
VERSION     DAOMYG010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13084_length_2846_cov_9.526335"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            424..1947
                     /locus_tag="PK610_11825"
     CDS             424..1947
                     /locus_tag="PK610_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36671.1"
                     /translation="MLTIQKLISLLEPEHLREFKANLESTGNTLPLRLVDTLEKVNPL
                     TVESDDLCKAVYGDIEEKTRKKFFQLAHYTFRLSSFLARRYPFYLTHNIALIERKFNS
                     GKQEESLLYAENLLDIALKTEDFSTQTWVLKFLINFSHIREKRKEAIRQQQLLNEALR
                     NEAVVNEIQLYIREHLNFKDKKNLTASYSDTHLQFFAQFHDHPCFSARMISKYGSVYT
                     LSFLDDSKFYLPEVRKDLEELIDEIEKHNYVIFPFSDNILLNVDYLHLKHTIRDMNEE
                     EVVREATRITRKWNTLRFWQNHINTAQIVSLSIQASYFITHYCICYKENYREQIPAHI
                     RDNIDETVGICKNLIEQMKEEKDAHVRYINLNNIYCMLLLLCGKDKIKESVDTLEALM
                     INYQQIPFQKLYDAIFANLIIGYFSLDDQNGVSECYRRYEKLTAHRSKIEENDVTIKT
                     FYYVSQFRQTGRDQYVDKIKTLLHEISDKSELSSTAQLIRDLCHYYKVPVEVISKSA"
     gene            2016..2510
                     /locus_tag="PK610_11830"
     CDS             2016..2510
                     /locus_tag="PK610_11830"
                     /inference="COORDINATES: protein motif:HMM:NF039825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HRJ36672.1"
                     /translation="MFYILSAEMIKAFIMGLPTWVIVLLCIVIFMGVVGQWALYAKCD
                     LPGYGALVPVWNVQLFLKIVGRPKEQGWIVMLPPVVALLCLLFIPNILAGCIIAGISL
                     LPWAWYMIKVFIEIAKCFGKKETSSFILIVLLNGMYLFNLALSQEEKYLGPIYKEGDK
                     PSIF"
BASE COUNT          853 a          686 c          566 g          741 t
ORIGIN      
        1 gaacaggaaa agaagaggga acaaacagag agaaaaagga tgcggattgc tttcatggct
       61 ttttgttaac tggtaaatgc aaaaatacca aaaggaaacg aaccgcgtaa aaacggaccg
      121 gggaccctaa ggtccccgat cgcactacta ctgcatgaaa agaacgtaca tcataaaatc
      181 atgcccctgc gccatcttca ccactctaaa cccctctaaa cagacgcgag cacaggcttt
      241 gccgaaggga tgctaaaaat tacacatcct ttccaaaaaa ggtgaaatgc cctttatcat
      301 tagcgaaatt aaggtgatgc gagggctaaa aaaattccgg tttgtttcaa aaatcagaaa
      361 ttaagaagga cgcctttcct gtttggtggt tttccttatt tttaggccac aactgcctgc
      421 aacatgctca ccatacaaaa actgatcagt ctccttgaac ctgaacatct ccgggaattc
      481 aaagcgaacc ttgagagcac aggcaataca ctgcccttac gtttggtaga taccctcgaa
      541 aaagtaaatc cgcttacggt agaatccgac gatctctgca aagcggtgta tggcgatatc
      601 gaagaaaaga cacgcaaaaa attctttcag ctcgcacatt acacttttcg tctcagctcc
      661 tttctggcac gacgctatcc cttttacctc acgcacaaca tcgcgctgat tgaacgcaaa
      721 ttcaattcgg gtaaacagga agaatcgctg ctgtacgccg aaaacctgct cgatattgct
      781 ttaaaaaccg aagatttttc cacgcagacc tgggtgctga aatttctcat caacttctcg
      841 catatccgcg aaaaaagaaa agaagctatt cgtcagcaac aactcctcaa cgaagccttg
      901 cgcaacgaag cagtggtgaa tgaaattcag ttgtacatcc gcgagcacct taatttcaaa
      961 gacaaaaaaa atcttactgc ttcctattcg gatacccacc tgcagttttt tgcgcagttt
     1021 catgatcacc cttgcttttc tgcacgtatg atttccaaat acggcagtgt gtacacgctg
     1081 agttttctcg acgattccaa attctattta cccgaagtac gcaaagacct ggaagaactt
     1141 atcgatgaaa ttgaaaaaca caattacgtg atcttccctt tctccgataa tattctgctc
     1201 aatgtagatt acctgcatct taagcacacc attcgcgata tgaacgaaga agaagtggta
     1261 cgtgaagcca ctcgtatcac ccgcaaatgg aacacactcc gcttctggca aaatcacatc
     1321 aacacggcgc aaattgtttc gctcagtatt caggccagtt atttcattac gcattattgc
     1381 atctgctata aggaaaatta ccgcgaacaa attcccgctc acatccgcga caacattgat
     1441 gaaacggttg gcatttgcaa aaatctgatc gagcaaatga aagaggagaa agatgcgcat
     1501 gtccgctaca tcaacctgaa caatatttac tgcatgctcc tgctgctttg cggaaaagat
     1561 aagatcaaag aatcggtgga tacgctcgag gcattgatga tcaattacca gcagattccc
     1621 tttcagaaat tgtacgatgc catttttgcc aatcttatca tcggctattt ctcgctcgac
     1681 gatcagaacg gtgtaagcga atgctaccgc aggtacgaaa aactgaccgc ccaccgctca
     1741 aaaattgaag agaacgatgt caccatcaaa accttctact acgtgagtca gttccgccaa
     1801 accggacgcg atcagtatgt ggacaagatc aaaacgctgc tgcatgagat cagcgataaa
     1861 tcagagctct cctctaccgc gcaattgatc agggatttat gccattatta caaagttcct
     1921 gtagaggtaa tttcgaagtc ggcctaaatt tcgaccgaag gtttgcgttt cgaccgcatt
     1981 ttcattaaat ttctgctacc aaaaacccga aagctatgtt ctatattcta tccgcagaaa
     2041 tgatcaaagc ctttatcatg ggcttaccca cctgggtaat tgttctattg tgcatcgtaa
     2101 ttttcatggg cgtggtagga caatgggcat tgtacgccaa atgcgatctc cccggttatg
     2161 gtgcattggt tccggtttgg aatgtgcagc tctttctaaa aattgtaggc cgccccaaag
     2221 aacaaggatg gatcgtcatg ctgccacctg ttgttgcatt gctgtgtttg ctttttattc
     2281 ccaatattct tgcaggctgc atcatcgcag gaatttcact cctcccctgg gcatggtaca
     2341 tgatcaaagt atttatagaa atcgcaaaat gcttcggcaa aaaagaaaca tccagtttta
     2401 ttctgatcgt tttactcaac ggtatgtacc tcttcaacct cgctctctcg caggaagaaa
     2461 aatacctggg tccgatttac aaagagggag ataagccatc cattttttga agaggtgaac
     2521 ggtgattggt gatcggtgaa cagtgatctg tgatcagtga acagtgaacg gtaagtaaaa
     2581 agcatcgaga tttagtgaga actccttagt gtttctcagt gacctccgtg ccttagtggt
     2641 gaaaaaaacc cactccaact aaccttccct ccttccactt gcactgagcc tgccgaagtg
     2701 tccatctgtg aaaaaaaact tagtgtttct cagtgacctc cgtgccttag tggtgaaaaa
     2761 accacactcc aactaacctt ccctccttcc acttgcactg agcctgccga agtgtccatc
     2821 tgtgaaaaaa aacttagtgt ttctca
//
LOCUS       DAOMYG010000413         2840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_13117_length_2840_cov_7.656732, whole genome shotgun sequence.
ACCESSION   DAOMYG010000413 DAOMYG010000000
VERSION     DAOMYG010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13117_length_2840_cov_7.656732"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="PK610_11835"
     CDS             complement(<1..1254)
                     /locus_tag="PK610_11835"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRJ36673.1"
                     /translation="MQKGHYQWIFVLLLGLGVQMSYAQTVRFSGKVVDKQTGEPLQGA
                     VVYIHETGKHAVCKEDGSFSLDNLKPAVYHIHASMLSYEPFAQNFRVNKDTIVVIAME
                     PTAIELKNFTVESEMVKTEAVKSSLSIESANKEFIEKQAGNTFMNALEKMPGISSMNV
                     GVGISKPVIRGMSFNRVVVTENGIKQEGQQWGADHGLEIDQFNVERVEILKGPASLLY
                     GSDALGGVINILPPPVVQEGKMNVHALGFFRSNNNASGTSLLAEGTRKRIFFRARITL
                     QEFGDYKVPADSFTYNGYKLPIYNQSLKNTAGKELNYSLSAGVMRDWGMFRISFTEFS
                     QQIGFFPGALGIPRSYSLQPDRSTKNIDIPGQSIFHRKFMVNFNIKTKRGWWENDWGY
                     QINYRTEKSLPHAHGVGYIDPANTTA"
     gene            1398..1811
                     /locus_tag="PK610_11840"
     CDS             1398..1811
                     /locus_tag="PK610_11840"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRJ36674.1"
                     /translation="MKKAADILIDKDLRKTPCRVFVLGEFIRKGNKGISETELENRSK
                     GKFDRVTIYRTLKTFMDADILHKVIDDDNLVKYALCKECEDDHHSHEHVHFKCTSCNT
                     TSCLDEISIVSVDLPKGYKKIEANYLIIGTCPECK"
     gene            1817..2752
                     /locus_tag="PK610_11845"
     CDS             1817..2752
                     /locus_tag="PK610_11845"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HRJ36675.1"
                     /translation="MKKNQIKALIPASVGFILFLLACTGKKQSLQEQLGYEYAENENS
                     LLWEISGNGLAEPSYLYGTIHIQRKEVFDYDTLVGLLVDTCAAYAMELNMEEMNPFKV
                     AKMMALDEPLDKLIPVEKYNQLDSFFRAETGTGIGLRKTTKPFFLMSEIMMKEIGGDM
                     PMALDLHFFSQAKSKKKKVIGIEKFEEQMAAVDELTVEEQVDIILKGLGDSTSSLNKF
                     DELIETYLKGDLEGMVRLTQDTLYPPKFNKAFLIDRNIVMADRIADICNKQVTFNAIG
                     AGHLGGPEGVIALLRKKGYTVKPIKTTFGNKQLKK"
BASE COUNT          856 a          608 c          569 g          807 t
ORIGIN      
        1 tgccgttgta tttgccgggt caatataacc aacaccatgc gcgtgaggca acgacttttc
       61 tgttcgataa ttgatctggt aaccccagtc attttcccac caacctcttt ttgttttgat
      121 attgaaattc accatgaatt tccggtgaaa aatggattgt ccgggtatat caatgttctt
      181 ggtagagcga tctggctgca gggaatacga tcggggaatt ccaagtgccc cggggaaaaa
      241 accgatttgc tggctaaatt ctgtaaatga aatccggaac attccccaat cgcgcatcac
      301 tccagcactc agtgaataat ttaattcttt gccggctgta tttttcagac tctgattgta
      361 aatcggtagt ttgtatccat tgtatgtaaa tgaatccgct gggactttgt aatcgccaaa
      421 ttcctgcaat gtaattcttg ctcggaaaaa gatccgttta cgtgttcctt cagccaacaa
      481 tgaagtcccg cttgcattgt tattgctacg aaaaaatccc aaagcatgga cgttcatttt
      541 accctcctga acaacaggtg gtggaagaat atttataacc ccaccaagtg catcactccc
      601 atacaataat gaagcgggac cttttaaaat ttcaactctt tcaacattga actgatctat
      661 ttccagacca tgatcagctc cccattgctg accttcctgt tttattccat tctcagtaac
      721 caccacgcga ttaaaactca ttcccctgat aaccggcttg gaaattccca caccaacatt
      781 catactgctg attccaggca ttttttccaa cgcattcatg aatgtgtttc ctgcttgttt
      841 ttcgatgaac tccttgttgg ccgattcaat actcagtgag ctttttacag cttctgtttt
      901 taccatttct gattcaactg tgaaattttt caattcaatg gctgtcggtt ccatcgcaat
      961 aactacaatg gtatctttgt tcactctgaa attctgcgca aatggctcat aactgagcat
     1021 tgaagcatga atatggtaaa cagccggttt caaattgtcg agcgaaaatg aaccatcttc
     1081 tttgcaaacc gcgtgctttc ccgtttcgtg aatataaact actgcaccct gtaaaggttc
     1141 tccagtttgt ttgtcgacta ccttaccact gaaacgtaca gtttgagcat aactcatttg
     1201 cacccccaag cccaggagca aaacgaatat ccattgataa tgaccttttt gcaagattgt
     1261 tgtttttgca atagtgttgc aaatatataa taaaaggacg taaacccttg taccatttgt
     1321 cacaccctgc gaccgtttgt gtggaagacg gtttccctgt tcctgccaaa gcctgttttt
     1381 ggtatattta acccttcatg aaaaaagcag ccgatatact tatcgataag gatttacgca
     1441 aaacgccatg cagggttttt gtgttgggtg aattcattcg gaaagggaat aagggaattt
     1501 ctgaaacaga gctcgaaaac cgaagcaaag gaaaatttga tcgtgttacg atttaccgca
     1561 cgttgaaaac atttatggat gcagatattc tgcataaagt aattgatgat gataatctgg
     1621 ttaaatatgc attgtgtaaa gaatgcgaag acgatcatca cagtcatgaa catgttcatt
     1681 ttaagtgcac ttcctgcaac accacaagtt gtttggatga gatatccatc gtatctgtag
     1741 atcttcccaa agggtataaa aaaattgaag ccaattattt aattataggg acttgtcccg
     1801 aatgcaaata acaaccatga aaaagaacca gatcaaagca cttattccgg cttctgtcgg
     1861 atttattctc ttcctgcttg cctgtaccgg caaaaagcaa agtttgcagg aacaattggg
     1921 ctacgaatac gccgaaaacg aaaactcgtt gttgtgggag atcagcggaa acggattagc
     1981 cgaaccgtcg tacctgtatg gaacgattca cattcagcgc aaggaagtat tcgattacga
     2041 tacacttgta ggattattag tggacacctg tgctgcttat gcgatggagt tgaatatgga
     2101 agaaatgaat ccgtttaaag ttgccaaaat gatggcgctg gatgaaccgc tggataaatt
     2161 gattcccgtt gaaaagtaca atcaactcga ttcctttttc cgtgcagaaa ctggaacggg
     2221 aattggattg cgaaaaacaa ccaaaccatt ttttctgatg tcggaaatca tgatgaagga
     2281 gattggaggc gatatgccaa tggcgctcga tctgcatttt ttctctcagg cgaaaagcaa
     2341 aaagaaaaaa gttatcggta tcgaaaaatt tgaagaacaa atggctgcgg tagatgagct
     2401 gacggtagaa gaacaagtcg atatcatcct gaaaggacta ggggattcaa cctcttcgct
     2461 gaataaattc gatgagttaa ttgaaaccta cctcaaaggc gatcttgaag gaatggttcg
     2521 gctcacacaa gacaccttgt accctccaaa attcaacaag gcctttctta tcgacagaaa
     2581 tattgtgatg gcagatcgca ttgcggatat ctgtaataaa caggtcacct ttaacgctat
     2641 cggtgctggt cacctgggcg gaccagaggg cgtaattgca ttactacgca aaaaagggta
     2701 cactgtaaaa cccatcaaaa caacattcgg aaacaaacaa ctcaaaaaat aaaaccatga
     2761 aacggatcag cttactactg ggtggatatc acctttgctg cctttgccga tgcgcctgaa
     2821 gaacccaaaa taccacgaac
//
LOCUS       DAOMYG010000414         2839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_13123_length_2839_cov_10.880388, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000414 DAOMYG010000000
VERSION     DAOMYG010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13123_length_2839_cov_10.880388"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /gene="radA"
                     /locus_tag="PK610_11850"
     CDS             complement(<1..1346)
                     /gene="radA"
                     /locus_tag="PK610_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016649981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRJ36676.1"
                     /translation="MAKVKSAYFCQSCGYESVKWLGKCPSCAAWNTFVEEVLDKEEKG
                     SFRPGASGSKRLAKAQKITEIRLQEEDRVLLSDRELNRVLGGGLVPGSLILIGGEPGI
                     GKSTLMLQIAVKNQGLKVMYVSGEESEKQIRMRGERIGFANPDCFILTETNTQNIFRQ
                     IEELQPDLLIVDSIQTLHTSYIESSPGSISQIRECTAELMRFAKESNTPVFLIGHITK
                     DGSIAGPKVLEHMVDTVLQFEGDRNHVYRLLRSVKNRFGSTNELGIYEMRGAGLREVE
                     NPSEILISNNDEGLSGIAIAATLEGMRPLLVEVQALVSTAAYGMPQRSATGFDAKRLN
                     MLLAVLEKRCGFKLGSKDVFLNIAGGLRVDDPAIDLAVVCAVLSSNVDLSVPSKTCLS
                     AEVGLSGEIRPVNRVEQRISEAEKLGYERIIISKYNKGISQDQFSIELIQVGKIEEV"
     gene            complement(1399..1878)
                     /locus_tag="PK610_11855"
     CDS             complement(1399..1878)
                     /locus_tag="PK610_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRJ36677.1"
                     /translation="MDLLAFAKRAAALKAENKKFLSSLKKSDPRKLDEKFHAQHEEVF
                     SRIDCLSCANCCKTTSPIVRESDVDRLAKTLRIRPAEVIAHYLTSEPDGTYVMRTSPC
                     PFLGADNYCSVYEGRPAACREYPHTDRKKMIQLLDLTFKNTQVCPAVLEMVEGMKQL"
     gene            complement(1885..2400)
                     /locus_tag="PK610_11860"
     CDS             complement(1885..2400)
                     /locus_tag="PK610_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HRJ36678.1"
                     /translation="MNKFDSIQSKILSLNDLQRSVKVWRLKSSKIVFTNGCFDILHRG
                     HATYLAMAAEYGNKLIVAVNSDASVRRQGKGDDRPVNDETSRALLIASLQVVDAVVVF
                     DEDTPLELIQMLQPDVLVKGGDYDAAETNPSSKKYIVGSDGVRANGGEIVTIPFVDGF
                     STTSTIAKLKG"
     gene            complement(2695..>2839)
                     /locus_tag="PK610_11865"
     CDS             complement(2695..>2839)
                     /locus_tag="PK610_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="addiction module antidote protein, HigA family"
                     /protein_id="HRJ36679.1"
                     /translation="TALRLARYFGTSPKFWMGLQDDYDLEEEMRAKQKEIAGIPRAKV
                     NAA"
BASE COUNT          763 a          675 c          649 g          752 t
ORIGIN      
        1 acttcttcaa tttttccaac ctgaattaat tcaatagaaa actgatcctg tgaaattccc
       61 ttattgtatt tggaaatgat tatccgctca taaccgagct tttctgcttc cgaaatgcgt
      121 tgttctacgc ggttcacagg acggatttca ccgcttaagc ctacttctgc agagagacaa
      181 gttttggatg gaacagaaag atcaacattc gatgataata cggcgcaaac tactgccaga
      241 tcaattgcag gatcatcaac acggagtcca cctgctatat tgagaaaaac atctttcgat
      301 ccaagtttaa atccgcaacg tttttcaaga accgcaagca acatgttcag tcgctttgca
      361 tcgaatccgg tggcggagcg ttgtggcatt ccgtatgctg ctgtacttac gagtgcttgt
      421 acttccacga gtaagggacg cataccttcg agtgtagcag caatagcgat tccgcttaat
      481 ccttcatcat tatttgagat cagtatttca gatgggtttt ccacttcgcg caaaccagca
      541 ccgcgcattt cgtaaatacc gagctcattg gtggaaccaa aacgattctt cacactgcgc
      601 aacaaacggt aaacgtgatt gcgatcgcct tcaaactgca atacggtatc gaccatgtgc
      661 tctaaaacct tcggaccggc gatgctgccg tctttggtga tgtgtccgat gagaaacacc
      721 ggtgtattgc tctccttcgc aaaacgcatg agctctgcgg tgcattcacg gatctgcgat
      781 atgctgcctg gtgaggattc gatgtaggag gtatgaagcg tctggatgga atcgacgatg
      841 agcagatcag gctgaagttc ttcgatctga cggaagatat tttgcgtatt ggtctcggtg
      901 agaatgaaac aatcgggatt ggcaaagccg atgcgctctc cacgcatgcg gatctgtttt
      961 tcgctttctt cgccactgac gtacataacc ttcaaaccct ggttcttcac tgcaatctgc
     1021 agcatgagag tggatttacc gataccgggt tccccaccga taagaataag cgagccgggt
     1081 acgagtccac cgcccaaaac acggttcagt tcacgatcgg agagtagcac acggtcttct
     1141 tcctgcagac ggatctcagt gattttttgt gctttcgcta aacgtttgga acccgatgcg
     1201 ccaggacgaa aacttccttt ttcttccttg tcgagaacct cctccacaaa ggtgttccag
     1261 gctgcacagg aaggacattt acccaaccat ttgacagatt cgtagccaca gctctgacag
     1321 aaatatgcgg atttcacttt agccatggga cgaaattagt gtaaatgcac gcaaagtatg
     1381 tgcgttgtgg aaaaagcttt acaactgctt catcccctcc accatctcca acacggccgg
     1441 acaaacctgc gtgtttttaa aggtgagatc gagcaactga atcatttttt ttcggtcggt
     1501 gtgtggatac tctctgcatg ctgcgggtcg accttcgtac acggagcaat agttatctgc
     1561 accaagaaaa gggcagggtg aagtacgcat cacataggtg ccatcgggtt cggaggtgag
     1621 gtagtgagca atgacttcgg caggacgaat gcgcagggtt tttgccaggc ggtccacatc
     1681 gctttcgcgg acgatcggac tggtggtctt gcagcaattg gcgcaggaga ggcaatcaat
     1741 gcgtgaaaac acttcttcgt gttgcgcatg aaatttctca tcaagtttgc gcggatcact
     1801 ttttttgagg gaggaaagaa attttttgtt ctccgctttc aacgcagcag cacgtttggc
     1861 aaatgcaagg agatcgatct gcactcagcc tttcagtttt gcgatggtag aggtagtgga
     1921 aaatccatcc acaaaaggaa ttgtaacgat ctctcctccg tttgcacgaa caccatcgga
     1981 acccacaatg tatttctttg acgaaggatt ggtctctgca gcatcgtaat ctccgccttt
     2041 taccaaaacg tccggctgaa gcatttgaat caattcaagt ggggtatcct catcaaaaac
     2101 cacaactgca tccaccacct gcaaagaggc gatcagcaaa gcacgcgatg tttcgtcgtt
     2161 gaccggacga tcatctccct tgccttgtct gcgcaccgaa gcatcggagt tcaccgcaac
     2221 gatcagctta tttccgtatt cggcagccat ggcgaggtag gttgcatgtc cgcgatgcag
     2281 aatatcgaaa caaccgttgg taaaaacaat cttcgacgat ttaagtcgcc ataccttcac
     2341 cgagcgttgc agatcattca gcgaaaggat tttggattgg atgctgtcga atttattcat
     2401 gctgttgtat ttacccgcgg ctttggatgc gaaaggtagc cattacctgg aagaaaaaaa
     2461 acatcctttt tcgcaatgtg gaactcctga attttacgta tactcgtaac aatgagctac
     2521 ctgatcatcg aaataaaaaa tccgaaagcg cgtaaactgt taaacgattt acgcgaccgg
     2581 aatcttattt ccatcaatga ggatagttct gtgttttttg aaaccgtcag aaaaataaaa
     2641 aagaaggcgg atagaaaaag attacactca aaaaacactc ttgcgcaata acaattatgc
     2701 cgcattaacc ttagcacggg gaatgcctgc aatttctttt tgttttgccc gcatttcttc
     2761 ctccagatca taatcatcct gcaatcccat ccagaattta ggagaagtac cgaaatagcg
     2821 ggccaatcgt aaagcggta
//
LOCUS       DAOMYG010000415         2837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_13142_length_2837_cov_7.763120, whole genome shotgun sequence.
ACCESSION   DAOMYG010000415 DAOMYG010000000
VERSION     DAOMYG010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13142_length_2837_cov_7.763120"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PK610_11870"
     CDS             complement(<1..342)
                     /locus_tag="PK610_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRJ36680.1"
                     /translation="MSTIIEQGHVKATVANGIAQVEFFHPMSNSMPGKLLNQLADTIT
                     EMGSNSDVKVIVLRSSGDKAFCAGASFDELISIKDLETGKKFFSGFANVINAMRKCPK
                     FIIGRVQGKAVG"
     gene            complement(436..771)
                     /locus_tag="PK610_11875"
     CDS             complement(436..771)
                     /locus_tag="PK610_11875"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRJ36681.1"
                     /translation="MSYWKEPLPATPYYAVIFISTRSYQLEGYPEMDELTIRLATEMD
                     GFIGYESINSDRKGIFISYWRDMKSIEEWRKHPVHLEAKKKGNSLWYDHYISQICKVE
                     HAHEHRLNS"
     gene            complement(938..1123)
                     /locus_tag="PK610_11880"
     CDS             complement(938..1123)
                     /locus_tag="PK610_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36682.1"
                     /translation="MKRSTITSIVILLIMIYPFYLGYLAEEKSDMAGLIGFLITVFGF
                     LIIMTITAFGGESRESH"
     gene            complement(1432..>2837)
                     /gene="paaZ"
                     /locus_tag="PK610_11885"
     CDS             complement(1432..>2837)
                     /gene="paaZ"
                     /locus_tag="PK610_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019974018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HRJ36683.1"
                     /translation="GILDHVSFQDVVTFTGSAHTGKMLKSHPRIVEEAVPFNMEADSL
                     NAMVLGEDVKPGSAEFDIFIKAAANEITVKAGQKCTAVRRLIIPADKVEDVQIALGKR
                     LASTVIGDPAKEGVRMGALAGKAQVQEVTEKIELLRKSQEIVFGDLNQFDLVGADKST
                     GSFLPPVLFLNKDPFKHTDCHNIEAFGPVSTIMPYKGIDEAIELAAMGKGSLVTSIIT
                     ADDRIAREYVIGAAPYHGRIHILNEKCAKESTGHGSPMPLLTHGGPGRAGGGEEMGGK
                     RGVFHYLQRTAIQGHPTTLTNVTETFMAGAEYKETPIHPFRKHFEELEIGETVFTHKH
                     TVTDADIVNFANVSGDNFYAHMDATSLEGTIFEGRVAHGYFVLSKAAGLFVDPKKGPV
                     LLNYGLDEARFTKPVYPGMTIGVKFTVKEKIDQEKRSADDIAKGIVKFLVDVYDETGE
                     TVAIATILTMVKKMDQK"
BASE COUNT          805 a          607 c          569 g          856 t
ORIGIN      
        1 acctacagct ttaccctgaa cgcgtcctat aataaacttc ggacatttac gcatggcatt
       61 gattacgttg gcaaatccgg aaaagaattt tttaccggtt tcaagatctt taatcgatat
      121 caattcgtca aatgaagcgc ctgcacaaaa tgctttatca ccggatgaac gaaggacgat
      181 caccttaaca tcggagttac ttcccatttc ggtgatggtg tctgcaagtt ggttgagtaa
      241 tttccctggc attgaattgc tcatgggatg aaaaaactca acctgtgcaa taccatttgc
      301 gaccgttgct tttacatgtc cttgttcgat gatcgtgctc ataagtacgt tttgcccaaa
      361 attaggattt tcccttacat agtcgggcca ttttgtggag gaagtgaatc tttaaaacct
      421 atacttaaat caggctcagg aatttaatcg gtgctcatgg gcatgttcaa ccttgcagat
      481 ctgactgata tagtggtcgt accataggga attccctttt ttctttgctt cgagatgtac
      541 aggatgtttt ctccattcct caatggattt catatctctc cagtacgaaa taaagattcc
      601 tttgcggtca gaattaatac tttcataacc gataaatcca tccatttctg tcgcaaggcg
      661 aatggtaagt tcatccattt ccggataacc ttcgagctga taagagcggg ttgaaatgaa
      721 aattacagca taatagggtg tagctggcaa aggttctttc caataggaca taacaatcgg
      781 ttaatggtag taaaagtgaa gccccctttt ttattcagga tctctggtca caatgcacta
      841 aaatagcaat tgtcattgta taaaaagcca aaggccatgg agaacccaca gcctttagca
      901 ccaaaaacca gaagagagga taaaaagaac gagaaattta gtggctttct ctcgattcgc
      961 caccaaaagc ggtgattgtc ataattataa gaaatccgaa tactgtaatc aggaatccaa
     1021 ttaatcctgc catatccgat ttttcttctg caagataccc caggtaaaaa gggtatatca
     1081 taattagaag aatgacgatg gatgtgattg tgctgcgttt cataacatca gatttgatga
     1141 tacaaactta cagccggcac atgcactaaa atatgagggt ggtcaggatg aagactgacc
     1201 ttaatcagga aaaaaatggt gaggtgtttt tgttattatt ccaaatggaa aacacgaaat
     1261 tttcgcaaat caaaacacac tcataattag ggtgattgga tcataaaaat agcaacacct
     1321 cccatttaga aaaatgaaat cgtccaattt gaacaatttg aatttcaatt taccaaatgt
     1381 gaattagttc acaggaaatg gaattagtta gccttaaaac agcgtttcaa ttcatttttg
     1441 atccatcttc ttcaccatcg ttaaaatggt tgcaatcgct actgtttctc cggtttcatc
     1501 gtaaacatct accaggaact ttacaatacc ttttgcaata tcatctgctg atcgtttttc
     1561 ctgatcgatt ttttccttta cagtgaactt cactccaatg gtcattccgg gataaactgg
     1621 tttggtgaat cgcgcttcgt cgagtccgta attgagcaga acgggccctt ttttcgggtc
     1681 aacaaataat cctgctgcct tggaaagaac aaaatatccg tgcgcaactc ttccttcgaa
     1741 aatggtgcct tcaagtgaag tggcgtccat gtgcgcgtaa aaattatcac cgcttacatt
     1801 ggcaaaattt acaatatccg catcggttac tgtgtgttta tgagtgaaaa cggtttcacc
     1861 aatttccagt tcttcaaaat gtttgcggaa ggggtgaatg ggtgtttcct tgtattctgc
     1921 acctgccata aatgtttcgg taacattggt aagcgtagtg ggatgtccct gtatggcggt
     1981 gcgctgaaga taatggaata cgcctcgttt tcctcccatt tcttcaccac ctccggctct
     2041 gcccggacct ccatgtgtaa ggagtggcat tggtgatcca tgtcctgtac tttctttcgc
     2101 acatttttca tttaaaatat ggatcctgcc atgatagggt gctgcaccta tcacatattc
     2161 ccttgcaatg cgatcgtcag cagtgatgat ggaggtaaca agcgatccct tgcccattgc
     2221 ggcgagttct atggcctcat ctatcccttt gtagggcata atagtagaca caggtccaaa
     2281 cgcttcaata ttgtggcaat cggtatgttt gaatggatcc ttattcaaaa agagaacagg
     2341 ggggaggaat gatcccgttg atttgtcggc tccaacaaga tcgaattggt taagatcgcc
     2401 aaacactatc tcttgcgatt tgcggagaag ttcaattttt tctgtcactt cctgtacctg
     2461 cgcttttccc gcaagtgcac ccatgcgcac tccttctttt gcaggatctc caattacagt
     2521 tgaagcaagc ctttttccaa gtgcaatttg tacatcttcc actttgtcgg caggaataat
     2581 taaccgacgg acagcagtac atttttgtcc ggctttaacc gtgatttcat tcgcggctgc
     2641 cttaatgaaa atatcaaatt ctgcagaacc cggtttaaca tcttctccaa gtaccatagc
     2701 attgagtgaa tcggcttcca tgttaaaagg gacagcttct tccacaatgc gcggatgcga
     2761 tttcagcatt ttcccagtat gtgcagaccc ggtaaatgtt accacatcct gaaatgaaac
     2821 atgatcaaga attccat
//
LOCUS       DAOMYG010000416         2822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_13235_length_2822_cov_9.022407, whole genome shotgun sequence.
ACCESSION   DAOMYG010000416 DAOMYG010000000
VERSION     DAOMYG010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13235_length_2822_cov_9.022407"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="PK610_11890"
     CDS             complement(<1..1496)
                     /locus_tag="PK610_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRJ36684.1"
                     /translation="MKTGLLAFALLFAFSFGSAKENKWNTNQTHGPSKSVSFTTSEGT
                     WMNLDISPDGKNIVFDLLGDIYILPITGGKAKLLRGGHAWEIQPRFSPDGKKISFTSD
                     SGGGDNIWVMDASGENARQVTTEDFRLLNNAVWTPDGNYLIARKHFTATRSLGAGEMW
                     MYHISGGEGVRLTQRKNEQQDVNEPCVSPDGKYLYFSEDMYPGGYFQYNKDPNNQVYI
                     IRRYNLTTGKLENLIGGAGGAMRPQISRNGKMLAFVRRIRTKSVLFVRDLSSGEEWPI
                     YDALSKDQQEAWAIFGVYTGFNWTPDDQQIVVWAGGKIRKINVHQAKSGPMGVQMNTV
                     EEIPFTADVKIEIEEALRFRQQIDPDVFTVKVIRNAVTSPDEKLLVFNAAGYLWKKEL
                     PNGKPERLTVSKEQESEPAFSPNGQELVYVNWNDERGGSLLKYQFKTKKSIPLSKSRG
                     IYRQPSFSPDGKMIVFVLEGGNEDMGFTFGTEPGIYRMDAAGGDPVLVT"
     gene            complement(1570..1821)
                     /locus_tag="PK610_11895"
     CDS             complement(1570..1821)
                     /locus_tag="PK610_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRJ36685.1"
                     /translation="MSELNEKIELAIAKIRPYLEADGGNIELVGVTPDFVAEVRLLGA
                     CSSCSMSAMTMRAGVEETIRKEVPEIRAVVAINDHSVEV"
     gene            complement(1821..2804)
                     /locus_tag="PK610_11900"
     CDS             complement(1821..2804)
                     /locus_tag="PK610_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRJ36686.1"
                     /translation="MSFTVRINNPALHSRKRMEEACRFAIERFLGNTYKVSVNVVALG
                     ADRSPDQRSILPGVKNIVAIASGKGGVGKSTITANLAAGLAKKGYKVGLIDADIYGPS
                     MPIMFDVQHEKPVALEIDGRSMIKPVESYGVKLLSIGFFAELDQAIVWRGAMASKALT
                     QMFRDTHWGELDYMLIDLPPGTGDIHLSLVQLVPLTGAVVVSTPQQIALADAKKGVSM
                     FQLDSIKVPVLGMIENMAYFTPAELPENKYYIFGQGGARQLAEQIDVPFLGEVPLVQS
                     IRESGDAGRPAVLQEGTPTAIAFDLIVANFVERVNWRNTHMAPTKPVEMQG"
BASE COUNT          771 a          689 c          556 g          806 t
ORIGIN      
        1 gttactaaaa cagggtcgcc tcctgctgca tccattctgt aaattcctgg ctcggttcca
       61 aatgtgaaac ccatatcttc atttccgcct tcaagcacga aaacaatcat ttttccatcc
      121 ggagaaaaag agggctgacg atagattcca cgcgattttg ataatggaat tgatttcttc
      181 gtcttaaact gatacttcaa aagcgatccg ccacgttcgt cgttccaatt cacataaacc
      241 agttcctgac catttggcga aaatgccggt tcggattcct gttccttcga aacagttaat
      301 cgttcaggtt ttccgttggg caattctttt ttccacaaat atcctgctgc attaaaaaca
      361 agcaattttt catccggtga tgttaccgcg ttgcgaatta ctttaactgt gaatacatcc
      421 ggatcaattt gctgacgaaa tcgcagcgct tcttcaattt caatttttac gtctgcagta
      481 aaaggaattt cttcaactgt attcatctgc acacccatcg gtccggattt ggcttggtgc
      541 acatttattt ttctgatttt ccctccggcc caaacaacaa tttgctgatc atcgggtgtc
      601 cagttaaatc cggtatacac tccaaaaatt gcccatgcct cttgttgatc tttacttaag
      661 gcatcgtata tcggccattc ttcaccactg gaaagatcgc gtacaaaaag gactgatttc
      721 gtacgaatac gccgcacgaa tgcaagcatt tttccattcc ttgaaatttg cgggcgcatt
      781 gctcctcctg caccaccgat caggttttcc agtttaccgg ttgtaaggtt atacctacga
      841 ataatataaa cctgattgtt gggatccttg ttgtactgaa aatatcctcc ggggtacatg
      901 tcctccgaga aatagaggta tttcccatca ggagaaacgc atggttcatt tacatcctgt
      961 tgttcgttct ttcgctgcgt gaggcgcaca ccttcgcctc cgctgatatg atacatccac
     1021 atttctccgg cacccagaga acgcgttgct gtgaaatgct ttctggcgat cagataattt
     1081 ccgtcgggag tccagactgc attattcagt aaacggaaat cttcggtggt gacctgtcgt
     1141 gcgttttcac cggaagcatc cattacccag atattatcac cacctccgga atcggaagtg
     1201 aaacttattt tttttccatc cggactaaat cggggctgta tctcccaggc atgaccacca
     1261 cggagtaatt ttgcttttcc gccggtaatt gggaggatgt aaatatctcc taacagatca
     1321 aaaactatgt ttttaccatc ggggctaata tccaggttca tccaggtacc ttcactggtt
     1381 gtaaaggaaa ccgatttgga ggggccatga gtttgattcg tattccattt attttcttta
     1441 gccgaaccga aagaaaatgc aaacaacaat gcaaaggcta ataagccagt cttcatagta
     1501 atggtattgg gttatggtta aaattaacca attccaatca ctaaacaacc gatgtgggaa
     1561 agaataagat cagacctcga cggaatgatc gttaatggcg acgacggcgc gtatttcagg
     1621 aacttccttg cgaatggtct cttcaacacc ggcacgcatg gtcattgcgc tcatggagca
     1681 actgctgcaa gcaccaagca agcgaacttc agccacaaaa tcaggtgtaa caccaaccag
     1741 ttcgatatta cctccatctg cttccagata aggtcggatt ttggcaatag ccaattcaat
     1801 tttctcgttt agctcagaca ttagccttgc atttcaaccg gtttggtggg tgccatgtgc
     1861 gtgtttcgcc agttaaccct ctctacaaaa ttagccacaa taagatcaaa agcaatagca
     1921 gtaggcgttc cttcctgtaa aacagccggc cgaccagcat caccgctttc acgaatactt
     1981 tgaaccaagg gaacttctcc aaggaaaggg acatcgattt gctctgcaag ctgacgtgct
     2041 ccgccctgcc caaaaatgta gtatttgttc tccggaagtt ctgcaggagt gaaatatgcc
     2101 atgttttcaa tcatacccaa tacaggtacc ttaatggaat ccagctgaaa catcgaaact
     2161 cccttttttg catctgccag tgcaatttgc tggggtgttg aaacaacaac agcaccggta
     2221 agcggaacca attgtaccaa actcaaatga atatcgcctg ttccgggagg taaatcaatt
     2281 agcatataat ccagttcgcc ccaatgggtg tctctgaaca tttgtgttag tgcctttgat
     2341 gccattgcac ctctccaaac aatagcctga tccaattccg cgaaaaaacc tatcgacaac
     2401 agttttactc cataactctc gaccggcttt atcattgatc gcccgtcgat ctccaaagca
     2461 acaggttttt catgttgaac atcaaacatg ataggcatgg atggaccata aatatcggca
     2521 tcaataagac caactttata cccctttttt gccaagcctg cagccagatt tgcagtaata
     2581 gtagatttac caactccacc ttttccggaa gcgatcgcta caatattttt gactccgggt
     2641 aaaattgaac gctgatcagg agagcgatca gcaccgagcg ctactacatt taccgaaacc
     2701 ttataggtat ttccaagaaa acgttcaatt gcaaatcgac aggcttcttc cattcgctta
     2761 cgtgaatgta aagcagggtt gttgatccgg actgtaaatg aaatttcatt gttgtccgcc
     2821 tt
//
LOCUS       DAOMYG010000417         2820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_13251_length_2820_cov_10.992043, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000417 DAOMYG010000000
VERSION     DAOMYG010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13251_length_2820_cov_10.992043"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PK610_11905"
     CDS             <1..248
                     /locus_tag="PK610_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36687.1"
                     /translation="NLYADKKQAEKDAKKADNKAKVDNSKLKVKIKVLKQEIASNEKS
                     LNKEKAKKKQNPEKIAQLEKDLAEKKTRLAEMEAQLR"
     gene            complement(356..1423)
                     /locus_tag="PK610_11910"
     CDS             complement(356..1423)
                     /locus_tag="PK610_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36688.1"
                     /translation="MKKFLVFTLAIALVFSSCKRDKGDTIDPPVTDPGLTASTPVYGY
                     GMLNRIKGIWSGPVTSTTSLGGFPDWTVDLRPIAPGQISSKSELDSLNDIFMSFFVTR
                     YNNEYRIAFRNGGGFAGSQRISYLQCDSVSETAQSAYYRFSDFVAGQARAWSEFRFSN
                     DSLYMRTYTSRYNTVAPAQLHMTWTAKLKDSTSAHIADSLFDFPRKVLAKDLSNAFST
                     MSESIFFNTSTEPYPENDQPYLGKTTVNISFSGAITPAAGTKNYVIFTTQPLFSGFSF
                     LSQNLKFRSRYVMLPAEDNQYQFTYMHPGTYYAYVLHDANGDGNFTSGDHINTPLSTI
                     SFTLSALGTETVNVQVNFTIP"
     gene            complement(1427..2020)
                     /locus_tag="PK610_11915"
     CDS             complement(1427..2020)
                     /locus_tag="PK610_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36689.1"
                     /translation="MVKHIRTLAILLVLFAVGKSNAQEGKQYIHKGLFCARGALSYGK
                     MFELSANQIYLDGNIEFYLDEKVSLRGSGYWFLSSFNGKTPLKLHHSLFAGPLYHFKT
                     KSSFDPYIGLEPGINLGQASDPCFGDPCLQTLLPYEMEMVATPLISPSIGFNYYGQKW
                     FHLWLSARYIAGRFMDNYNSASLNEFRISFGLGFNIN"
     gene            complement(2014..2649)
                     /locus_tag="PK610_11920"
     CDS             complement(2014..2649)
                     /locus_tag="PK610_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36690.1"
                     /translation="MRTVLILFLFIPFAAHSQESGKFEGSGTLQVGLRTTSSLFGDAG
                     YSGFGTGGQFRLRLGKRLNTEWFADYILTDIGGLGRRADGHIGWSVMFYPFSTGEKKI
                     TPYFLAGHCFDYTRVTPFNTLVKDNSGQSQQRWSSATQMGLGAHWHITEKFDISFSAQ
                     YMIHLGKDLHYHIEEVNGIKELQMAPPHDDATLEGHLLLTASLNIRIADLW"
BASE COUNT          829 a          561 c          660 g          770 t
ORIGIN      
        1 ggaatttgta tgccgataaa aagcaagccg aaaaagatgc aaaaaaagca gacaataagg
       61 ccaaagttga taacagcaag ctaaaagtga aaatcaaagt gctgaaacag gagatcgcat
      121 caaatgaaaa atctctgaac aaggaaaaag ccaagaaaaa acagaatccg gaaaaaattg
      181 cgcaattgga aaaagacctg gccgaaaaga aaacacgctt ggcagaaatg gaagcacagt
      241 tgaggtaaat acattcggaa aaatagtcgt tttcgacaat ttcagaaaaa taaaaaataa
      301 aaggggcaat cggaattcaa ttccttttgc cccttttcaa ttgcggtgtt tttaatcaag
      361 gtatcgtaaa attcacctga acatttacgg tctctgttcc caatgccgac agcgtaaaac
      421 tgatcgtgct caggggagta ttgatgtgat ctcctgatgt gaaatttccg tcaccattgg
      481 catcgtgcag aacgtaggcg taataggtgc ccggatgcat ataggtgaat tggtattgat
      541 tgtcttctgc gggtaacatt acatagcgcg agcggaattt taaattctga cttaaaaaag
      601 aaaatccgga gaaaagcggt tgtgtggtaa aaatcacata gttttttgtt cctgctgccg
      661 gtgtaatggc tccagaaaaa ctgatgttga ccgttgtttt ccccaggtaa ggttgatcat
      721 tttccgggta gggctctgtt gatgtattga agaaaatgga ttctgacatg gtgctgaatg
      781 cattgctcag gtcctttgcc aatactttgc gcggaaaatc gaatagtgag tctgctatat
      841 gtgctgatgt gctgtctttt aatttggctg tccaggtcat gtgcaattgt gccggtgcaa
      901 ctgtattgta tctgctggtg taggtgcgca tgtacaaact gtcgttactg aagcggaatt
      961 ccgaccaggc tcttgcttgt ccggcaacaa aatctgaaaa acggtaatag gccgattgtg
     1021 cagtttctga tacgctgtcg cattgcaaat aggaaatgcg ttgtgatccc gcaaagcctc
     1081 caccattacg aaatgcgatg cggtattcat tgttgtatcg cgttacaaaa aacgacatga
     1141 aaatatcgtt cagcgaatcg agctccgatt ttgaagagat ctgtccggga gcgattggac
     1201 gcagatcgac tgtccagtcg ggaaatcctc caagcgatgt ggttgaggta accggtccgc
     1261 tccagattcc tttaattcgg ttaagcatac cgtatccgta aaccggggtg cttgcagtaa
     1321 gaccgggatc tgtaaccggt ggatcgatag tgtcgccttt atcgcgtttg caggaggaaa
     1381 atacaagagc aatagcaaga gtaaatacca ggaacttttt cataaatcaa ttaatgttaa
     1441 atcctaatcc aaaggaaatt ctgaattcgt tgagcgaggc gctgttgtaa ttatccatga
     1501 atcttcctgc aatataccgg gcactgagcc atagatgaaa ccacttctga ccgtaataat
     1561 taaatccgat ggagggcgaa atcagtggcg ttgccaccat ttccatttcg taaggcaata
     1621 atgtttgcag acaaggatca ccaaaacagg gatcgctggc ctgaccaaga ttgattccgg
     1681 gttcgagccc gatataagga tcgaaactgg atttcgtttt aaaatgataa agtggtcctg
     1741 caaacagcga gtggtggagt ttcaaaggtg ttttcccatt gaacgaagaa agaaaccagt
     1801 atccgctgcc gcgcagggaa actttttcat cgaggtaaaa ttcaatattg ccgtcgagat
     1861 agatttgatt cgctgataat tcaaacattt ttccatacga caatgctcct cttgcgcaga
     1921 atagtccttt atggatgtat tgttttcctt cttgtgcatt gctttttccg actgcaaaaa
     1981 gtacaagtag aatggctaat gtgcggatgt gttttaccat aaatctgcga ttcggatgtt
     2041 aagactggcg gtgagtaata aatgtccttc tagtgttgca tcatcatgtg gcggtgccat
     2101 ttgtagttcc ttgattccgt ttacttcttc aatatggtaa tgaaggtctt taccgaggtg
     2161 aatcatgtat tgcgcagaaa aggaaatatc aaatttctcc gtgatgtgcc aatgcgctcc
     2221 caaacccatt tgggtggcag agctccagcg ttgctgactt tgtccggagt tgtcttttac
     2281 caatgtgtta aacggtgtta ctctggtgta atcgaaacaa tgtccggcaa ggaaataagg
     2341 tgtaattttt ttctctcctg tagaaaaagg ataaaacatt acactccaac caatatgtcc
     2401 gtcggccctg cgacccaatc ctccgatgtc ggtgaggatg taatcggcga accattcggt
     2461 gttgagacgc ttgccaagac ggagtcggaa ttgtccgcct gttccaaaac cgctgtaccc
     2521 cgcatcgcca aacagactgc tggtggtgcg taatccgacc tgcagagttc cggagccttc
     2581 aaacttgccg gattcctgag aatgagcggc aaagggaata aaaagaaata aaatcagaac
     2641 agtcctcata caatcttgtt ttatatccta acgacaacac ttttgccaaa ggttgcatga
     2701 aaagggtgaa aattcttttc atgatgatgc aagataagta aaatcggacc gtaattttgc
     2761 cttcatgatc aagcgattct caattctgac gaagctcacc ctgatagctg ttctgctggt
//
LOCUS       DAOMYG010000418         2791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_13446_length_2791_cov_8.766813, whole genome shotgun sequence.
ACCESSION   DAOMYG010000418 DAOMYG010000000
VERSION     DAOMYG010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13446_length_2791_cov_8.766813"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PK610_11925"
     CDS             complement(<1..532)
                     /locus_tag="PK610_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013071710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRJ36691.1"
                     /translation="MASFRYFILFKPFGMLSQFSREGDHPALSDLAFSFPKDCYPVGR
                     LDHDSEGLLLITNDKGLNHQLLDPQYKHQRTYWVQVEGHINEEAIGKLESGVEISING
                     KKHKTLPAKAKAIQAPALPERNPPIRFRKNVPDSWLELALTEGKNRQVRRMTAATGFP
                     TLRLVRSAIANLKLGTL"
     gene            complement(538..1206)
                     /locus_tag="PK610_11930"
     CDS             complement(538..1206)
                     /locus_tag="PK610_11930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HRJ36692.1"
                     /translation="MSSNYIRNIEYLCRKHKTDQSTLADTIGIDFTDLRRPTPDDLTR
                     IADHFEITLDHLIRIPVYRIDEIRSKNIKLFVTDVDGVLTDGGMFYTESGDEFKKFNA
                     KDGLAIKALQAKGIHTGIISHGINKDLIGRRAALLDVKLVEVGTTPKLDILKAWCSQL
                     GIELNQVAYIGDDLTDLDCIKAVGLSACPADAVDAIKRHALVVTRRNGGDGCVRELLD
                     DYLL"
     gene            complement(1209..2204)
                     /gene="aroF"
                     /locus_tag="PK610_11935"
     CDS             complement(1209..2204)
                     /gene="aroF"
                     /locus_tag="PK610_11935"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRJ36693.1"
                     /translation="MILQLKPGTSEQLAHELAERFSAFCVFDGGHFLLVTSSSQHQAD
                     PETEPYVERIQTFTDDMQLSSRSWQKPTREVIVGNSHFGNGKTTVVMAGPCSVESEHQ
                     IREISGMLVEQGLGTLRAGAFKPRTSPYTFQGLGKKGLELLLKMKQEFGLNIITEVRD
                     STHIEEVIEAADIVQIGAKAMYDQGILRRCGKSDKAVMIKRGFGSTLQELVQAAEFVM
                     SAGNLRVLLCERGIRTFETKTRFTLDLCGVAWLKEKCNLPVILDPSHAMGYAYGVPDL
                     TRACTAMGVDGLLIEVHPDPANALSDASQQLTPQQFRDLVASLQPIAQAVGRKIS"
     gene            complement(2217..>2791)
                     /locus_tag="PK610_11940"
     CDS             complement(2217..>2791)
                     /locus_tag="PK610_11940"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRJ36694.1"
                     /translation="CKDVQMSKGLRFFKEFLKERKTVGALSPSSIFLARKMLAPIDFE
                     KARVIVELGPGTGIFTKEVLKKLHPDAKLLVFETQKNFCEMIERDIKDSRMILINDSA
                     EKIQEYLQKNGLENADYTISSIPFTVVPSQIKNTILSQAVKCLKPGGIFIQFQYSLNA
                     HKLLKSYFKTVKLDFTPMNIPPAFIYKCSV"
BASE COUNT          728 a          626 c          623 g          814 t
ORIGIN      
        1 gaagtgttcc caatttaagg tttgcaattg ccgatcgcac taaacgcaat gtcggaaacc
       61 ctgttgctgc tgtcattcgc cttacctgtc tgttttttcc ctctgtaagt gcaagttcca
      121 gccacgaatc cggaacattt tttcgaaacc tgatcggcgg atttcgttcg ggtaatgctg
      181 gtgcctgaat tgctttggct tttgcaggta aggttttgtg ttttttcccg ttgatgctga
      241 tttcaacacc gctctcaagt ttgcctatcg cttcttcgtt gatgtgtcct tccacctgca
      301 cccaataggt ccgctggtgt ttatattgtg gatccaggag ttgatgattc agtcctttgt
      361 catttgtaat cagcaacaat ccttccgaat catggtcgag tcttccaaca ggataacaat
      421 cttttgggaa ggaaaaagct aaatcactta aagcggggtg atctccttcg cgactgaact
      481 gagagagcat tccaaatggt ttgaagagta taaagtagcg gaatgaagcc atgcttttta
      541 cagcagataa tcgtcgagca attcgcgtac acatccatca cctccgttgc gacgggtaac
      601 aacaagtgca tgccgcttaa tcgcgtccac tgcatcagcc ggacaagcag agagtcctac
      661 tgctttgata caatccagat cggtaagatc atcaccaata taggcaacct ggttcagttc
      721 aattccgagt tgcgaacacc atgctttgag aatatccagt tttggagtgg ttccaacttc
      781 aacaagtttc acatcgagca acgcagcacg ccgaccaatc agatctttgt tgatgccgtg
      841 actgataata cctgtatgta tgccctttgc ttgtaatgct ttaatggcca atccgtcttt
      901 tgcattgaat tttttgaatt catctccact ctcggtataa aacataccac catccgttaa
      961 aactccatca acatcagtta caaaaagttt gatgtttttc gaacgaatct catcgattct
     1021 gtaaacagga attcgaatca gatgatccag tgtaatttca aaatgatcgg caatcctggt
     1081 cagatcatca ggtgttggtc ggcgaagatc ggtaaagtca attccgatgg tatccgccaa
     1141 tgtgctttgg tcggttttgt gtttgcggca caggtattcg atattgcgga tgtaattgga
     1201 actcatagtc aggaaatttt tcgtcctact gcttgtgcaa tgggctgcaa agatgcaaca
     1261 agatcgcgga attgttgagg agtgagttgt tgtgatgcat cgctaagtgc attggcagga
     1321 tcgggatgta cttcaatgag caatccatca actcccatag ccgtgcatgc ccgggtaagg
     1381 tcgggaacac cataggcata acccatagcg tgactgggat cgagtattac aggaagatta
     1441 catttctctt tcagccaggc cactccgcat agatcgagtg taaatcgggt tttggtttca
     1501 aatgttcgaa ttcctctctc gcaaagcaga acccgaaggt taccggccga catcacaaat
     1561 tcggctgcct gaaccaattc ctgtagcgtg gatccgaatc cacgtttgat cattacggcc
     1621 ttatccgatt ttccgcagcg gcgcaaaatt ccctgatcat acatggcttt tgctccgatc
     1681 tgaacaatat ctgcagcttc aatgacttcc tcaatatggg tggaatcgcg cacttctgtg
     1741 atgatgttta gtccgaattc ctgtttcatt ttcagaagca gttccaatcc ctttttgccc
     1801 aatccctgaa aagtgtaagg cgatgtacgg ggtttgaaag ctccggcacg taaggttccc
     1861 agtccttgtt ccacaagcat acctgatatc tcgcggatct ggtgctccga ttctacggaa
     1921 cagggaccgg ccataacaac agtggtttta ccattgccaa aatgactatt tccaacgata
     1981 acttcacgtg taggcttttg ccagcttcgg gaggagagtt gcatatcatc cgtaaaggtc
     2041 tgaatcctct caacgtaagg ttctgtttcc ggatcggcct ggtgttgtga agaagaagtt
     2101 acgaggagaa aatgcccgcc atcgaagaca caaaaggccg agaatcgttc ggccagttca
     2161 tgtgctaatt gttcggaagt accgggttta agttgtagaa tcaaatgctt taaaatttaa
     2221 acgctgcatt tgtaaataaa cgccggcgga atattcatgg gtgtgaaatc gagcttcacg
     2281 gttttgaagt aggatttaag caatttgtgc gcattcagcg aatactgaaa ctgaataaaa
     2341 ataccacccg gtttaaggca tttaacagcc tgtgaaagga tagtgttttt tatttgcgat
     2401 ggtactacgg taaacgggat ggaggaaatg gtgtaatcgg cattctcaag gccgtttttc
     2461 tgaaggtatt cctgaatttt ttcggcagaa tcattaatca gaatcatgcg tgaatcctta
     2521 atgtcgcgct ctatcatctc gcagaaattc ttttgtgttt caaacacgag aagctttgca
     2581 tccggatgaa gcttcttaag tacctccttg gtaaagatgc cggtgcccgg acctaattcc
     2641 acaatgactc gtgccttttc aaaatcaatc ggagccagca tttttctggc aagaaaaatt
     2701 gaactgggag aaagcgcacc taccgtcttt ctctccttta gaaattcttt gaaaaatcgt
     2761 aatcctttac tcatctgaac atccttgcag g
//
LOCUS       DAOMYG010000419         2789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_13461_length_2789_cov_8.421361, whole genome shotgun sequence.
ACCESSION   DAOMYG010000419 DAOMYG010000000
VERSION     DAOMYG010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13461_length_2789_cov_8.421361"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PK610_11945"
     CDS             <1..498
                     /locus_tag="PK610_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HRJ36695.1"
                     /translation="NTPNLRELQEKEPLKKLLGGLMDQNLKKNKPKPILLKIAPDLTN
                     EQLDDIIEIVAETKIAGVIATNTTISREGLKTPVQKTENIGAGGLSGKPVKNRSTEVI
                     RYLSEKSNKSFPIIGVGGIHSPEDAIEKLNAGASLIQLYTGFIYEGPSLLKRINKSLL
                     QNNKA"
     gene            495..1355
                     /locus_tag="PK610_11950"
     CDS             495..1355
                     /locus_tag="PK610_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRJ36696.1"
                     /translation="MNSQVKLIECPRDAMQGLKEFVPTEKKIHYINQILRCGFDTVDF
                     GSFVSPKAIPQMADTEEVLAGLDLASTSSKLLAIVANVRGAEDACKHAVISYLGFPFS
                     ISETFQQRNTNSGIAESLERVKEIQNLCIKHNKELVVYISMAFGNPYGDVWNSDIAID
                     WCRKLNEEVGVKILALSDTIGISAPDNIDYLFRSLIPALPKVEFGAHLHTTPGTWKEK
                     IDAAWKAGCRRFDGAIKGYGGCPMAKDELTGNMPMENLLSYFKEIGMNSGINEEAFSS
                     AYSGSSEIFH"
     gene            1490..2125
                     /locus_tag="PK610_11955"
     CDS             1490..2125
                     /locus_tag="PK610_11955"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRJ36697.1"
                     /translation="MKYFVIFLLFLSVRMDVKAQGCFCDAAKHSSGNVHFGLVGRYGL
                     AGFNDGNLNATRTSFSPGFRIGTHITDVLTIQSGVDYYSFNRLNTNTPSYPFHNQSIV
                     EIPIHLIGRIAPDNGRIMPYFGLGFSSRFTPKSNYYSSLDNPDIGYSTEEKMSVNADA
                     MAGIWILISDQFSISAGLEHRQSLLPLVSEGVKAEHLKSFTSAQIGFQIHF"
     gene            2142..2747
                     /locus_tag="PK610_11960"
     CDS             2142..2747
                     /locus_tag="PK610_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36698.1"
                     /translation="MRQLLTFSLISVILFSCSSEKKKANPVEVSDFISEKILIDLSYP
                     PYQDTVIPQIMARLGLCELEGYSPLDTNKRAPCSHELFRVFMNHGNANWQDGFLVEVR
                     PGVYSPEFRLLSIARVEGVYKVTNDYAGELLEMRTTPEGMYDLIIRYVDGVVGTVAIF
                     HEWRTNHYEPITVVELNDHFVKEEKMDSLNSVYIGNFSWGF"
BASE COUNT          861 a          566 c          580 g          782 t
ORIGIN      
        1 aatacgccaa atctccgcga acttcaggag aaagaaccat tgaaaaaact actcggcggc
       61 ttaatggatc agaatctgaa aaagaataaa ccaaaaccaa ttttgctgaa aattgcaccg
      121 gatttaacca atgaacaact tgatgatatc atagagatcg ttgcagaaac taaaattgca
      181 ggggttatcg caactaacac tactatttca cgagaagggt taaagacacc ggtacaaaaa
      241 acagaaaata ttggtgccgg aggtttaagc gggaagccgg taaaaaacag atctacagaa
      301 gtcatccgtt acttatctga aaaatcaaat aagtcatttc caatcattgg agttggtgga
      361 atacattcgc ctgaagatgc aattgaaaaa ctcaatgcag gcgctagtct gatacaactt
      421 tatacaggat ttatttacga aggaccgtct ctcctaaaac ggataaacaa atcattgctg
      481 caaaacaaca aagcatgaac agtcaggtaa aattgatcga atgtccgcgc gacgcaatgc
      541 aagggctaaa ggaatttgtc cctacggaaa agaaaattca ctacatcaat caaatactgc
      601 ggtgcggatt tgatactgta gatttcggaa gttttgttag tccaaaagcc attcctcaaa
      661 tggcggatac agaggaagta ttggcaggtt tggatcttgc ttccacaagc tctaaattac
      721 tagctatcgt ggccaacgta agaggtgcgg aagatgcatg caaacatgct gtgataagtt
      781 atttaggatt tccattttcc atttcagaaa cctttcagca gcgaaacacc aattccggaa
      841 ttgcagaatc gctcgaacgc gtcaaggaaa ttcagaatct ctgtataaaa cacaacaaag
      901 aactggtcgt ttatatttcc atggcattcg gaaatccgta cggagatgtt tggaacagcg
      961 atattgcgat tgactggtgc cgtaaactga atgaagaagt cggcgtgaaa attctggcgt
     1021 tatcagatac cattgggatt tctgctccag ataacattga ttacctcttc cgttctctaa
     1081 ttcctgctct tcccaaggtt gaatttggag cccatttaca cactacccct ggaacatgga
     1141 aagaaaaaat agatgctgca tggaaggcag gatgcagaag atttgatgga gcaattaagg
     1201 gttacggagg ctgtccaatg gcgaaagatg aactaaccgg caacatgccc atggaaaacc
     1261 ttctcagcta ttttaaagag attggaatga attccggtat caatgaggaa gctttttcct
     1321 ctgcttattc cggaagctca gaaatattcc attaacaagc ttaaaaagct gattactaaa
     1381 ataactacga tgttagttat tttatttgtc cggttgtttt ccctgggttt ttagccacta
     1441 gtggaacgac aaacatgtaa cgaaacccga atttttccgt tttagctaca tgaagtattt
     1501 tgtgattttc ctgctctttt tatcggtccg aatggatgtg aaagctcaag gttgtttttg
     1561 tgatgcagcc aaacacagta gcggaaatgt acattttgga ttagtgggta gatatggcct
     1621 tgccggattt aatgatggga atctcaatgc cacccgaaca agtttttccc cgggatttag
     1681 aatcggtacg catattacgg atgtactcac tattcaatcc ggtgtagatt attacagttt
     1741 caaccggctt aacacaaaca ccccctcgta tcccttccac aaccaaagta tcgtcgaaat
     1801 tccgattcat ttaataggcc ggattgcacc cgataatggc aggataatgc cttatttcgg
     1861 tctcggattc tcttcaagat ttacaccaaa atccaattat tattccagtc tggataaccc
     1921 cgatatcgga tattccacag aagaaaagat gtcggtaaat gccgatgcga tggcaggaat
     1981 ctggatctta atttctgatc aattctctat tagtgcagga ctagaacacc gtcaatcctt
     2041 attgccattg gtcagtgagg gagtaaaagc tgagcattta aaatcattta cctcggcaca
     2101 aataggtttc cagatccatt tctgaacact atttttgcag catgcgacaa ctcctaacgt
     2161 tttcactgat cagtgttatc cttttttcct gttcatctga aaagaaaaaa gcaaatcctg
     2221 ttgaagtcag cgattttatc agtgaaaaaa tcctaatcga tctttcttat ccaccttacc
     2281 aggatacggt gattccacaa atcatggcca gactgggttt atgtgaattg gaaggttatt
     2341 caccgcttga caccaataaa cgtgcgccct gcagtcatga actttttcgt gtttttatga
     2401 atcacggcaa cgcgaattgg caggatggat ttctggttga agtacgacct ggggtttact
     2461 ctcccgaatt ccgcttgctt tccattgcac gcgttgaagg tgtgtataaa gtcaccaacg
     2521 attatgccgg cgaattactc gagatgcgaa ctactcccga aggaatgtac gatctgatca
     2581 ttcgctatgt agatggtgta gttggaacgg ttgctatttt tcacgaatgg agaaccaacc
     2641 attatgaacc catcacggtg gttgaattaa acgaccactt cgttaaagaa gaaaaaatgg
     2701 actcgctcaa cagtgtttac attggtaatt tctcctgggg attttagtga gaacggaatt
     2761 cagaatcttt agtattgcac tgctcacct
//
LOCUS       DAOMYG010000420         2788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_13471_length_2788_cov_8.645810, whole genome shotgun sequence.
ACCESSION   DAOMYG010000420 DAOMYG010000000
VERSION     DAOMYG010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13471_length_2788_cov_8.645810"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="PK610_11965"
     CDS             <1..1130
                     /locus_tag="PK610_11965"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ36699.1"
                     /translation="DFLQKEMIEKGEGAIPVLADYDGDGLLDLFVSSFGYFDPVSDSI
                     ICKISLYRNVGTATKPEYNFLTDNFEGLNSVIGQRSLVPTFGDLDGDGDDDMIVGDET
                     GVLARFVNTAGPGNPYDFILVSAGMLDYNGVLIDAGQFSAPVLVDIDRDSDLDILVGN
                     RTGKISFYENIGTTTLASFRLVTVSFGNLNVKEWWDNSGFSMPSVYDDNGSYQLFVGS
                     KAGWVHHYNNIDGNLSGSFTWVDSTVFEIPSGLRTAPAVADLNNDGFMDMILGNFRGG
                     LSFFVGDPQGSSSVQEYANIDFSIFPNPVSDQLFIAISDYIPGYRIIIHDLTGRELYS
                     GAINADRTAIDVSSFVPGLYFISIFNGSKTTTQKFIRYESR"
     gene            1117..1497
                     /locus_tag="PK610_11970"
     CDS             1117..1497
                     /locus_tag="PK610_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36700.1"
                     /translation="MKAVKFSLLIICLLVVFSSCERVAGKGGKAAIHVTTVNGPLNTS
                     GVSLRMKYGATSFPGENVPYDEDGFTDYAGDYTFNDLKRGDYYIYGKKITATDTLLGG
                     AHVLINNKPGETHVVIDFAEEDPF"
     gene            complement(1958..>2788)
                     /locus_tag="PK610_11975"
     CDS             complement(1958..>2788)
                     /locus_tag="PK610_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRJ36701.1"
                     /translation="GLAEGKALDMFQTASLLEFDTRVTGSTNDYSKTANSDVVVITSG
                     IPRKPGMTREELIGINAGIVKTVTENILKHSPNAIIIVISNPMDTMTYLALKSSGLPK
                     NRIIGMGGILDSARFKTYLSMELKCSPNDLHGVVIGGHGDTTMIPLMRLATLNGAPVA
                     NMLSVDKQKQIVADTMVGGATLTKLIGTSAWYAPGAAGALLVESIVRDQKKVFPCCVS
                     LEGEYGQKDICLGVPVVIGRNGWEKIIDYKLNAEEQDLFNKSADAVRNMNSVLDTLTV
                     "
BASE COUNT          754 a          525 c          610 g          899 t
ORIGIN      
        1 gggatttttt acaaaaggaa atgatcgaaa aaggcgaagg agctattcct gtgcttgcag
       61 attatgatgg cgacgggtta ttggatttgt ttgtttcctc gtttggttat tttgatcctg
      121 tgagtgattc gattatttgt aagattagtt tgtaccggaa tgttggtaca gcaacaaagc
      181 cggaatacaa ttttcttaca gataattttg aaggtttgaa ttcagtcatt gggcagcgca
      241 gtttggttcc gacttttggt gatctcgatg gtgatggtga tgatgatatg attgttggtg
      301 acgaaacagg tgtgttggca cgatttgtaa acactgcagg acccggaaat ccctatgatt
      361 tcattttggt atctgcagga atgcttgatt ataacggagt attgattgat gcaggacaat
      421 tttctgcacc tgtgttggta gatattgacc gtgattccga tttggatatt cttgtaggga
      481 atcgcacagg taaaatttca ttttatgaaa atattggaac aactacttta gcctctttta
      541 ggttggtaac agtttcgttt ggtaatctca acgtaaaaga gtggtgggac aatagtggat
      601 tcagtatgcc ctctgtttac gacgataatg gatcgtacca gttgtttgtc gggtctaagg
      661 ctggttgggt tcatcattat aacaatattg atggaaattt atccggttcc tttacctggg
      721 tagatagtac ggttttcgaa atcccttccg gattgagaac agctcctgcg gttgcagatc
      781 ttaacaacga cggatttatg gatatgattc tggggaattt cagaggaggc ttatcctttt
      841 ttgttggcga tccgcaaggc tcatcttccg ttcaggaata tgccaatatt gatttttcta
      901 tttttcccaa tcctgtttcc gatcaattgt ttatcgctat cagcgactac ataccgggtt
      961 acagaattat tattcatgat cttacaggac gggaattata ttccggcgca ataaacgcgg
     1021 acagaactgc aattgatgtg tcttcttttg ttccgggttt gtattttatt tctattttca
     1081 atgggtcaaa aaccacaacc caaaagttta tccgatatga aagccgttaa attctcctta
     1141 ttaattatat gcctgcttgt tgttttttcc tcctgcgagc gggtagcggg aaaaggcggt
     1201 aaggctgcga tccatgttac cacagttaac ggtcctttaa atacatccgg tgtatctttg
     1261 agaatgaaat atggagcaac ctctttcccg ggtgagaatg tgccttatga tgaagacgga
     1321 tttacagatt atgctggcga ttacacgttt aatgatctta agcggggtga ttactacatt
     1381 tatggaaaaa aaataacagc taccgatacc ttattgggtg gagcacatgt actaatcaat
     1441 aacaagccgg gcgaaacgca tgttgtaatt gattttgccg aagaagatcc tttttaacag
     1501 gtgaaagaaa acagaagaat cgtttttttc gatggcgttt gcaacttatg taattcatcc
     1561 gttcagcgga taatacgtaa cgacccgaaa gggaattttt attttgcctc gctacaatct
     1621 gattttgcaa aagatttttt agctacacga attcctgctg cagattcttc cctgggtact
     1681 ataatttatc atgacagtaa tggaaatctt catcagcgtt ccggagctgt tttgcggttg
     1741 acggctttga tgaaattccc ttatccctta ttgtattttt ttctcatcgt gcctcctttt
     1801 attagaaatg caatatacaa ttgggttgca aaaaacaggt acaaatggta cggtaaacgt
     1861 gatcagtgta tgataccgga tcaaaagtta aaagcacggt ttattgaata aaaaaaggag
     1921 gtaattaacc tccttttagt tttttgaaaa ggttcactta tactgtaagt gtatccaaaa
     1981 cagaattcat attgcgaact gcatctgcgc ttttgttgaa gagatcttgt tcttcagcgt
     2041 tcagtttgta gtcaatgatt ttctcccaac cgttacgtcc aattactacc ggaacgccaa
     2101 gacagatgtc tttctgaccg tattctccct caagagaaac acaacatggg aaaacttttt
     2161 tctgatcacg aacaatgctc tcaaccagca acgctcctgc cgcacccggt gcataccatg
     2221 cagaggttcc gataagtttg gttaatgtgg caccaccaac catagtgtcg gcaactattt
     2281 gtttttgttt gtcaacagac aacatattgg caaccggagc accgttaagt gtagccaaac
     2341 gcatcagtgg aatcattgtc gtatcaccat gaccgccgat aacaacgccg tgcaggtcgt
     2401 taggtgaaca cttcagttcc atgctgaggt aagtcttgaa tcgtgcacta tccagaatac
     2461 cacccatacc aataattctg ttctttggaa gtcctgaact cttgagtgcc aggtaggtca
     2521 tggtgtccat cggattggaa ataacaataa tgatggcatt gggtgagtgc ttgagaatat
     2581 tttcggttac agttttaaca atccctgcgt tgattccgat caattcttca cgtgtcattc
     2641 cgggtttacg gggaatacct gatgtaatca ccacaacatc tgaatttgca gtttttgaat
     2701 agtcatttgt actccctgta acacgagtat caaattcgag aagtgaggcg gtctggaaca
     2761 tatccagtgc ttttccttct gcaagacc
//
LOCUS       DAOMYG010000421         2731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_13866_length_2731_cov_8.189088, whole genome shotgun sequence.
ACCESSION   DAOMYG010000421 DAOMYG010000000
VERSION     DAOMYG010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13866_length_2731_cov_8.189088"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PK610_11980"
     CDS             complement(<1..304)
                     /locus_tag="PK610_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRJ36702.1"
                     /translation="MRIKFLLLFLFTLTLSQVKAQFGPDVDYALPTEYTIENITVTGA
                     IHFDERAIILLSELERGQKIKVPGDDISKAIKKLWDQKLFSEVAIDATQIRGDKIFL"
     gene            complement(294..1046)
                     /locus_tag="PK610_11985"
     CDS             complement(294..1046)
                     /locus_tag="PK610_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRJ36703.1"
                     /translation="MNLKEQIDPKRIPLHVAIIMDGNGRWAKQKGESRIMGHISGVES
                     VRESLTAATELGVKHLTLYAFSTENWNRPKEEVDALMNLLVNTIAQEIASLSENGVRL
                     KAIGDIDNLPESCKAALFKAIDQTSNNTTIELILALSYSSRWEIVNATRKIAEMVKKG
                     TLDPASISEDTIHQHLTTKGIPDPELLIRTSGEKRISNFLLWQCAYTEFYFTKTLWPD
                     FKKEHFYEAIAAYQQRERRFGMVSEQLNSDAH"
     gene            complement(1132..1959)
                     /locus_tag="PK610_11990"
     CDS             complement(1132..1959)
                     /locus_tag="PK610_11990"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRJ36704.1"
                     /translation="MLRFSKIFTIVLLLFVHSVKAQIELGLTGGVSYYIGDLNPYQHF
                     RSNHFAGGIFLRDNVNDRFAWRLSFTYGNVSGADSLSSNPNRSARNLSFRSRIIEIGP
                     IFELTFVKFSLGNITDKSATFYLLAGLTYFNMNPQGRLNGEYVDLQPLGTEGQGTNLN
                     SRRAYSLNQISIPLGIGMKGNISKRVCIGLEYGLRKTFTDYLDDVSGKYVDPNQLAIQ
                     NGPMAAHFANQSTDSPAIAGTLRGNPNTKDWYAFALATLSVRLGKLNKCYSFSSKRN"
     gene            complement(1963..>2731)
                     /locus_tag="PK610_11995"
     CDS             complement(1963..>2731)
                     /locus_tag="PK610_11995"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRJ36705.1"
                     /translation="RMKDLEFAMTRPAVDVMYAYKINPSFKARTHLLYGRLNGDDALT
                     SERFRNNRNLSFRSPVVELAFMVEYYPLTEKLGHIYALRGVKAKKKLKYLAPYISTGI
                     AGFWFNPRAKYPVDGKYYSLHKLGTEGQGLPGGAPSYKRISLCIPSGIGVNYMMNKYL
                     SIGFEIGGRLTFTDYIDDVSTVYYNNAAIQSARGDVAAYFADPSKQDAQLIADAGGNP
                     TRPGLQRGDKSDNDSYMFAIFRVNYRFLKGRFVLPKF"
BASE COUNT          749 a          571 c          591 g          820 t
ORIGIN      
        1 ctaaaaagat tttatctccc cgtatttgtg tggcatcaat agcaacttct gaaaaaagtt
       61 tttggtccca aagttttttt atcgccttac tgatgtcatc gcccgggact ttaattttct
      121 gtccgcgctc aagttctgaa agaagtatga ttgccctttc atcaaagtga attgcacctg
      181 taactgtgat gttttcaatg gtgtattccg ttggtaacgc atagtcaaca tcaggcccga
      241 attgagcttt gacttgtgat aaagtcagtg taaaaagaaa cagaagtagg aacttaatgc
      301 gcatcggagt tgagttgttc gctgaccatc ccaaagcgac gttcacgctg ctgataggct
      361 gcaattgctt cgtagaaatg ttcttttttg aaatcaggcc aaagtgtttt ggtgaagtag
      421 aattcggtgt aggcacattg ccagagcagg aaattactaa ttcttttttc accgctggtg
      481 cgaataagta gttcaggatc gggtatgcct ttagttgtaa ggtgttgatg aatggtgtct
      541 tcgctaatcg aagcagggtc gagagtacct ttctttacca tttctgcaat ttttcgtgtt
      601 gcgttgacaa tttcccaacg agaactgtag ctcaatgcaa gaattaattc aatggtagta
      661 ttgtttgagg tctgatcaat tgctttaaat aaagcagcct tgcacgattc aggtaaatta
      721 tcaatatctc caattgcctt taagcgcaca ccgttttcac taagagatgc aatttcctgc
      781 gcaatggtat ttaccagcag attcatcaaa gcatccacct cttcttttgg acgattccag
      841 ttttcagttg aaaaagcata aagcgttagg tgcttaaccc caagctcagt agcagcggtt
      901 agactttcgc gaaccgattc aacgccactg atgtgaccca taattcggga ctcacccttt
      961 tgcttagccc aacggccatt gccgtccata attatggcta cgtgcagggg tattcttttt
     1021 gggtcgatct gttctttgag gttcattggc ctgaaaacaa gcgcgcaaat atccgcatta
     1081 ttgttcggat tggaaaaaat taaccttttt tgagagttct tttctctgaa atcaattgcg
     1141 cttgctgctg aaactatagc atttattcag ttttcccagt cgaaccgaaa gggttgctaa
     1201 agcaaatgcg taccagtcct tggtattcgg gtttcctctt aaagttcccg caatggctgg
     1261 ggaatcagta ctctggttgg caaaatgtgc agccattgga ccattttgaa ttgcaagttg
     1321 attgggatcc acgtacttcc ccgacacatc atccagataa tcagtaaatg tctttcttaa
     1381 cccatattcc aatccgatac ataccctctt actaatattc cctttcatgc ctatgcctaa
     1441 aggaattgaa atctgattca gcgaataggc tctccgtgaa ttgagatttg tcccctggcc
     1501 ttcggttccc aagggctgaa gatcaacata ttccccgttg agccggcctt gtggattcat
     1561 gttgaaatag gtcagtccgg cgagaagata gaaagtagcg gatttatcgg tgatgttccc
     1621 aagggagaac tttacgaatg ttaattcgaa aatgggtccg atctcaatta ttctggaacg
     1681 aaagctgaga ttgcgtgccg aacgattagg gttggaactc aatgagtctg ctccagaaac
     1741 gttgccatag gtaaaagata aacgccaggc gaaacggtcg tttacgttgt ctcgaagaaa
     1801 aattcctcct gcaaaatggt tgctgcgaaa atgctggtag ggattgagat ctccaatata
     1861 gtaggagaca ccacctgtta aaccaagctc aatttgtgcc ttcacggaat gaacgaaaag
     1921 caataatacg atcgtaaaaa tttttgagaa gcgcagcatt tgtcaaaact taggaagaac
     1981 aaatcttccc ttgagaaaac ggtaatttac tcgaaatatt gcgaacatgt aggaatcgtt
     2041 atccgattta tcacctcttt gcagtccggg gcgggttggg ttgcctcctg catccgcgat
     2101 aagttgagca tcctgtttgc ttggatcagc aaagtaagca gccacatctc cgcgggcaga
     2161 ttgaatggct gcgttattgt aatatacagt gcttacatca tcaatgtaat cggtaaaggt
     2221 tagtcttcca ccgatttcaa atccgatact caggtattta ttcatcatat aattcacgcc
     2281 aattccggag gggatgcata gtgaaattct tttatacgat ggtgctccac cgggtaatcc
     2341 ctgaccttca gtccctaatt tgtgcaatga ataatatttc ccgtcaaccg gatatttcgc
     2401 gcgcggattg aaccaaaatc ctgcaatacc tgtagaaatg tagggagcca aatatttaag
     2461 cttcttcttt gcttttactc ctcgaagagc gtagatgtgt ccgagctttt ccgttaaagg
     2521 ataatattcc accatgaagg ctaactcaac taccggagag cgaaaagaaa gatttctgtt
     2581 gtttctgaag cgttctgaag taagtgcatc atcaccgttt agacggccat atagaagatg
     2641 agttcttgct ttgaaacttg ggttgatttt ataggcatac attacatcta ctgcaggacg
     2701 agtcatggca aactccaaat ctttcattcg g
//
LOCUS       DAOMYG010000422         2729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_13887_length_2729_cov_10.749065, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000422 DAOMYG010000000
VERSION     DAOMYG010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13887_length_2729_cov_10.749065"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PK610_12000"
     CDS             complement(<1..424)
                     /locus_tag="PK610_12000"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRJ36706.1"
                     /translation="MKQSITFFWHRRDLRIEDNHGLFRALSESDNVVPVFIFDTNILS
                     RLRAKDDQRVSFIHHYLTALKVKYKKLGVELLVKVGEPIQIWESLCEEFKVQAVYCNR
                     DYEPYALHRDRLVYDGLKEKGITFFGFKDHVVFEKAEVV"
     gene            512..>2729
                     /locus_tag="PK610_12005"
     CDS             512..>2729
                     /locus_tag="PK610_12005"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ36707.1"
                     /translation="MKRRRLLFLIALCYGLLTEPALCQMGSKSLNPRGEQTVPLRIQK
                     KNWDEIVSERTLHSSSFRKPDGTWVGYFSKELVNYYNTSGELVPVSTELCKTKNGWNV
                     PSQPHAFSLTRTGEIGVMLPGGESIILSSSAEINNHKLSNGTAFIHGDTAQFFGQAPG
                     IDKFIHFRLNGSKYSYIINNASILSSGDMIVKEKLFLPDGIDFLADPQMSESRNDRIA
                     GHLLALDKKGNELLRIASAFCYDASGSWTLAEYKFTRKGNEILLETIVPDSWLHDPAR
                     QFPLVIDPLVTGPTSTYPFQFMPSCFFPNYNVDSILVTVPPAITVNNLSVTGSYYADP
                     WTTTTMADGRMYYSTTCASSQVFTIAPPGGNTAGTAYLDDFNMRSPLMCCRPQQCNGY
                     TFYLRMHISRTINGAGCNTTFLYYNPSTAWPFSAFIEGNTPESYSSQMVLFPSTLCAN
                     DCETSASLYARYGVPPYTFSHPWATSSVTQGAPSGCSSGSQVGNLTLNIPNCPDYCDP
                     STSLPVPAAIITDACGNTVTGLPALSLTISPIAQASANPSSAIICSGEDVNMNLSACL
                     PGATINWFGNGLSGTGNITDTTFTNTGSSTISADYSAFITMNGCTSDTITINVAVEPQ
                     PNADFSTTPSPVFITQSTTFNDIVTSPGTIVNYYWDFGDGTTTSVQNPAHLYTAPGEY
                     VVCFSILTSTGCIDTICKTISVLPLEIIPPNVFSPNGDGQNDALVFPYLEFYAGNTL"
BASE COUNT          763 a          668 c          517 g          781 t
ORIGIN      
        1 tcacaacttc agctttctca aaaacaacat gatccttaaa accaaaaaaa gtaattcctt
       61 tttccttcaa tccgtcgtaa accaatctat ccctatgcaa cgcgtaaggt tcataatcgc
      121 ggttacaata aacagcctgc accttgaatt cttcgcacaa ggattcccag atttgaatcg
      181 gttcacccac ttttaccaac aactctaccc ctagcttttt gtatttcact tttaaggctg
      241 tgagatagtg gtgaataaaa ctcacacgct gatcgtcttt tgcccttagt cgggataaga
      301 tgtttgtatc aaaaatgaac accggaacga cattatccga ctccgaaaga gctctgaaaa
      361 gcccatgatt atcttcaatt cggaggtccc gcctgtgcca gaaaaatgta atcgactgct
      421 tcatacccca ataacaatca acgaagaaaa tagttctcca ttgcagaaat ctacgatttt
      481 gtttaaattg ggaatgctat tattgttgct tatgaaaagg agacgactac tgttcctgat
      541 cgcattgtgt tatggcttgc tgactgagcc cgcattgtgt caaatgggtt ccaaatccct
      601 taacccaagg ggagaacaaa ctgttcctct gcggattcag aaaaagaact gggacgaaat
      661 tgtatcagaa cgcacactgc attcctcttc gttccgaaaa ccagatggaa catgggttgg
      721 gtatttcagc aaggagttgg tcaattatta taacacatcg ggtgaattgg tacccgtgtc
      781 taccgaactt tgtaaaacca aaaacggatg gaacgttccc tcgcaaccgc atgcattttc
      841 acttacgcgc acaggtgaaa taggtgtaat gcttcccggc ggagaaagca tcattctcag
      901 cagctcggcc gaaatcaaca accacaaact ttcaaacggc accgcattca ttcacggcga
      961 tactgcacaa tttttcggac aagcaccggg tatcgataaa ttcattcatt ttcgcttgaa
     1021 cggaagtaag tacagttaca tcattaacaa tgcttccatt ctctcttccg gcgatatgat
     1081 tgtaaaagaa aaactgtttc ttccggatgg aatcgatttt cttgcagatc ctcaaatgag
     1141 tgaaagtcga aacgatcgca ttgccggaca tctccttgca ttggataaaa aaggaaatga
     1201 attacttaga attgcatctg ccttttgtta tgatgcttcc ggctcatgga cattagcaga
     1261 atataaattc acgagaaaag ggaacgaaat tctactcgag acaatagtac ccgacagttg
     1321 gctgcatgat ccagcccgtc aatttccatt ggtgattgat ccgcttgtta ccggaccaac
     1381 ttcaacctat ccttttcagt ttatgccttc ctgttttttt ccgaattata atgtagatag
     1441 catcctcgtt actgtacctc ctgctattac ggtaaacaat ttaagcgtaa ccggaagtta
     1501 ttatgcagat ccctggacaa ccacaactat ggccgacgga aggatgtact atagtactac
     1561 ttgtgcctca tcgcaggtat ttacaatagc accacccgga ggaaatactg caggaacagc
     1621 ctacctcgat gatttcaata tgagaagtcc gttaatgtgt tgtcgtccgc aacagtgcaa
     1681 tggctatact ttttatttac gcatgcatat ttcacgaacc attaatggcg ccggatgcaa
     1741 taccactttc ctgtattaca atccgagtac agcatggcca ttttctgctt ttatcgaagg
     1801 aaatactccg gaaagctatt cttcccagat ggttttgttt ccttccacct tatgcgccaa
     1861 tgattgtgaa acaagtgcat cgctctatgc gcggtatggc gttcctccct atacattttc
     1921 acatccctgg gcgacaagct ctgtcacaca gggagcacca tcgggttgct cgtcgggatc
     1981 acaagtgggt aacctaacct tgaatattcc aaattgtccg gattattgcg atccctccac
     2041 ctcacttcct gtgccagctg ctatcatcac tgatgcatgc ggaaataccg ttacaggttt
     2101 gcctgcgctt tcgctaacta tttccccaat cgcacaggcc agtgccaatc cttcttctgc
     2161 gatcatttgt tcaggtgaag atgttaacat gaatctcagc gcctgtctac ccggggctac
     2221 catcaactgg tttgggaatg gattgtccgg aaccggtaat atcacagata caacttttac
     2281 caatacagga tcctcaacca tctctgctga ttattccgct tttataacta tgaacggatg
     2341 tacatctgat acgatcacca taaatgtagc tgttgaacca caaccgaacg ccgatttcag
     2401 caccactcca tctcctgttt tcataacaca gtcaactaca tttaatgata tcgttacaag
     2461 tccgggcaca attgtaaatt actattggga ttttggagat ggaacaacaa catccgttca
     2521 aaatcctgca catctctaca cagctcccgg tgaatatgtt gtttgttttt ctatactcac
     2581 ttccacgggt tgcatcgaca ccatttgtaa aaccatcagt gtacttcctc tggagatcat
     2641 tcctcccaac gtttttagtc cgaatggcga cggtcaaaat gatgcgctcg tttttcccta
     2701 cctggagttt tacgccggca acacattat
//
LOCUS       DAOMYG010000423         2727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_13901_length_2727_cov_9.337949, whole genome shotgun sequence.
ACCESSION   DAOMYG010000423 DAOMYG010000000
VERSION     DAOMYG010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13901_length_2727_cov_9.337949"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PK610_12010"
     CDS             complement(<1..283)
                     /locus_tag="PK610_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36708.1"
                     /translation="MHNKTIKVAIAEGDCFFSILLRHYFESNPDFNFSDHLRTYEEVV
                     NYEKWESVILLLNYTALGANGVKKIRGILDMHPNARILCYSIYYDPILEI"
     gene            459..872
                     /locus_tag="PK610_12015"
     CDS             459..872
                     /locus_tag="PK610_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRJ36709.1"
                     /translation="MNKHVLLKKQKALSLTEVLIVMVIIGILLMVALPNQTALISQAK
                     SQEAKLQLTHLHTLQKQYFYLNSRYSNDINEIGFEQELLVTAGGNANYKIDLVDASPS
                     GFKARATAVIDFDQDGQINVWEIDEKKMLKEVTRD"
     gene            884..1408
                     /locus_tag="PK610_12020"
     CDS             884..1408
                     /locus_tag="PK610_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36710.1"
                     /translation="MIIVSLCINAALILVLLSVVVTDFRGRYVVVSQLLGVFVLGGIS
                     ILINGSFDQLSLAVIQNCFVSLLLVGLCGLYFRFVKGDSQFLDKYFGRGDLYFLLALS
                     PLIAPAYFIPFMICGLLIALFGSMLLSVIYDRQNGIPLAGWLAGFAIIVTTISGETWQ
                     WLFSEYSINISGHG"
     gene            1401..>2727
                     /locus_tag="PK610_12025"
     CDS             1401..>2727
                     /locus_tag="PK610_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008639550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRJ36711.1"
                     /translation="MVEDGLLELSPELQSLITPEQAWYYRIIPCTQTSSEIIFYTDSG
                     FFRDELLEEIELVLGKSVRVKKIESHLIQRTLSRFYRKAQGHGKIIQVQSGQADDFLS
                     KLIIEARQLGSSDIHIEIYDEKARVRIRIDGMLVERYKVSRLDYPALINKIKIRAQLD
                     IAEKRLPQDGRIRFNESGLKVDIRVSVLPTLHGEKIVLRLLGNDAANIEIGNLGFNET
                     ELKRYLEGIKRPNGILLISGPTGSGKTTTLYATLKILNEEKRNIVTVEDPIEYTLEGI
                     NQVQLKESIGLGFASALRTFLRQDPDIIMLGEIRDGETAQMAIRAALTGHLVLSTIHT
                     NSAWGTVSRLIDMGVPPFLLSQTLNTSVAQRLLRLLCPHCKQEAPLAENALPPAFQPP
                     QKLETHFIPVGCEQCFDTGYKGRKAIYEIIPVDFSVSEMIRRNENHIQPL"
BASE COUNT          763 a          515 c          592 g          857 t
ORIGIN      
        1 gtatttcaag aatgggatcg taatagatgg aataacataa aattctggcg ttcgggtgca
       61 tatcaagtat tcccctgatt ttttttacac cattggcccc tagtgcggtg tagtttaata
      121 ataggataac cgattcccat ttttcatagt taactacctc ctcatatgtt cgaaggtgat
      181 cggaaaagtt gaagtccgga ttggactcaa aataatgccg aaggagaatt gaaaaaaagc
      241 aatcaccttc agcgatggct actttaatgg ttttgttgtg cattggttcc gtttttgcga
      301 caattatata gtttatttaa ttatttaaat caaaatattg ctacaaaatt tgggtattta
      361 ttacacttct aactgagtat atatactcaa aaatatatag ccttaagggg gtataagaga
      421 tttctcggag ttctgtaatt gtggtaacta gcgcatgtat gaataaacat gtccttctca
      481 aaaagcaaaa agccttatcg ctaaccgaag ttttgatcgt gatggtcatc atcgggattt
      541 tgctcatggt tgcccttccc aatcaaacag cgttgatctc gcaagccaaa tcgcaggaag
      601 caaaattgca acttacccat ctgcacactc ttcaaaaaca gtatttctat ctcaattcca
      661 ggtacagtaa cgatattaat gaaattggtt ttgaacagga attactggtc actgccggcg
      721 gaaatgcaaa ctataaaatc gatttagtag atgcaagtcc ttccggtttc aaggcccgtg
      781 caacagctgt aattgatttt gatcaggatg gtcagatcaa tgtttgggaa attgatgaaa
      841 agaaaatgtt gaaggaagta acacgcgatt aaataacctg tgcatgatca tagtttcact
      901 ttgcattaac gcagcattaa ttcttgtttt gttatctgtt gttgttacag atttcagggg
      961 gcgctatgtt gttgtgtctc agcttttggg agtgtttgtg ttaggtggga tcagtatcct
     1021 catcaatggt tcatttgatc agttgtcgct tgctgtaatt cagaattgtt tcgtttcact
     1081 tttgctggtc ggcctatgtg ggctttattt tcgattcgtt aaaggtgatt ctcaatttct
     1141 tgataagtat tttggtagag gggatcttta cttccttctt gccttatcgc cacttattgc
     1201 accggcgtat tttattccct ttatgatttg cggattgctc attgcacttt ttggttcaat
     1261 gcttctgagt gtaatttacg accggcaaaa tggaattcca cttgcaggat ggcttgccgg
     1321 atttgccatc attgtgacca caatttccgg tgaaacctgg cagtggcttt tttctgaata
     1381 ttccattaat atttccggtc atggttgagg acggattact tgaactcagt cctgagctcc
     1441 aatcgcttat tacacctgaa caggcgtggt attaccggat tattccttgt acgcaaactt
     1501 catctgaaat aatcttttac acagattccg gattctttcg tgacgaattg ctcgaagaaa
     1561 ttgaattggt acttggtaaa tctgttcggg ttaaaaaaat tgagtcgcac ctcattcaac
     1621 gcactctaag ccgattttac cgcaaggctc aaggtcatgg caaaatcatt caggtgcagt
     1681 caggccaggc tgatgatttt ctttctaaac tgattattga ggctcgtcaa ttgggttcaa
     1741 gtgatattca cattgagatc tacgatgaaa aagccagagt gcgaattcgt attgatggta
     1801 tgttggtgga gaggtataaa gtcagccgtt tggattaccc cgcattgatt aataaaatta
     1861 agattagggc acagctggat attgccgaaa aaagattgcc gcaggatgga cgcatacggt
     1921 tcaatgaatc cggactcaag gtggatatcc gtgtttctgt gttgcccacg cttcacggag
     1981 agaaaattgt acttcgccta ttagggaatg atgccgccaa tattgaaatc ggtaatctcg
     2041 gttttaatga aaccgaattg aaaagatacc ttgaaggcat aaagcgtcct aacggaatat
     2101 tattgatttc cggtcccaca ggatcaggta agactaccac cttgtatgca acactgaaaa
     2161 ttcttaatga ggaaaaacgg aatatcgtta cggttgaaga tcccattgag tatacccttg
     2221 aagggattaa tcaggtgcag cttaaagaaa gtattggtct tggttttgcc agcgcattgc
     2281 gtactttttt acgtcaggat ccggatatta ttatgttagg cgaaatccgg gatggagaaa
     2341 cagctcaaat ggcaattcgt gcagcgctta ccggacattt ggttctatct acgatccaca
     2401 caaattcggc ttggggaacc gtatcgcgat tgatcgatat gggagttcct ccctttttac
     2461 tttcgcaaac actgaacaca agcgtcgcac aacgattgct gcgtttattg tgtccgcact
     2521 gcaaacagga agcaccactt gcagagaatg ctttacctcc tgcatttcag cctcctcaga
     2581 aattggaaac gcattttatt cccgtaggat gtgagcaatg ttttgatacc ggttacaaag
     2641 ggcgaaaagc gatctatgaa attataccgg tggatttttc ggtgagcgaa atgatccgga
     2701 gaaatgaaaa ccatatacaa ccattat
//
LOCUS       DAOMYG010000424         2723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_13935_length_2723_cov_7.690405, whole genome shotgun sequence.
ACCESSION   DAOMYG010000424 DAOMYG010000000
VERSION     DAOMYG010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13935_length_2723_cov_7.690405"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2723
                     /locus_tag="PK610_12030"
     CDS             <1..>2723
                     /locus_tag="PK610_12030"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRJ36712.1"
                     /translation="KQINCDKLEVTKKEWEKLYVDKQTNQIYSGMAQCEVEGKMLFLS
                     VKDGQLHGKQINCDKLEVTKKEWEKLREYSWYFEHKGTYYKMLKEVRYEKGEEQESVL
                     CKYYASGQIKSRSNAFKGRKIFDQRSYYEDGTLQEYRFVNALGATSRYFFELDEKGTM
                     QSKGFTFGETDTELRVDASVREFAVGDRMGLKVKGKVVFPAENEMLIAFPEVGLVMIE
                     NGGTSFLYKIADKGLLCNDTFNVVYPFAENGIAQVLLNNGKEGFIDRNGTYVVMPEYD
                     RIDRLTNGYWQVFVGEDRSGICSPEFKMILPAEYLRLEREKDIVLAKKKEGMQLMDTL
                     YRLLNPDVFEEISTMYEHPFLEIKKNGKKGMMSYSGEVLVPPVYSDVYLWRSESFYTF
                     KSGDSTYTLLGADGRIIVHDQLSFDYLKFLPNGAAIANVGGASILVSKSEKVLIPCCN
                     YWIDNLVENDSVKAVHVETGEAFKASIYNGGLTRLKRSCLDEYEDYKVSKISENQFII
                     PAGEDKYVYADSLCNPKSKAYHSIYANGIVKLNEKYGLLDSSAKEVIQPIYLNYSYGG
                     QYVAFKSVDGWELFGRNGMKLVPYKFEEIEYVGLEHLNVKQAGKWGLLSLDGRKNSMV
                     IPAKYSRIDLLENNALAVSDRGLFGVFDTTGKEILPMIYKDITALKKAYYALDRPSVV
                     ERILQFRNSIGGFSFMEGTDQMLTYDSEWITFWDLKSNTEIGHFTLNNTPHGDAMGYQ
                     TIKHAGYLDPLNIYAEFYISGGGGHYYRVLNTFDQGASIPHNMSTIEFQLPIPGSGGK
                     AVLGKDEGTRVDLYRYDPIDHSFKLWHKGEEGKDVHTYRVNTTPNHNSTHFGYYDQIS
                     KKYWVHSFSNPSLNYFLSREKYPEGSFVFSEKNLLLLQEGKFQKFNLTTGA"
BASE COUNT          828 a          421 c          691 g          783 t
ORIGIN      
        1 gaagcagatc aattgcgata agctggaggt gacaaaaaaa gaatgggaga agctgtatgt
       61 cgataagcag acaaatcaga tctattcggg aatggcgcag tgtgaggtgg agggaaagat
      121 gttgtttttg agtgtgaagg acggacaatt acacgggaag cagatcaatt gcgataagct
      181 ggaggtgaca aaaaaagaat gggagaagct gcgggaatat tcctggtatt tcgagcataa
      241 gggaacttat tacaaaatgc tcaaggaagt tcgctacgaa aaaggagaag agcaggagag
      301 tgtgctttgc aagtattatg cttcgggtca aatcaaaagt cgttcaaatg cctttaaggg
      361 gagaaagatc ttcgatcagc ggagttatta tgaggatggt actttgcagg aatatcgttt
      421 tgtgaatgcc ttgggtgcga cttcccgtta ttttttcgaa ctggatgaaa agggaacaat
      481 gcaatccaaa ggatttacat ttggtgagac cgacacggaa ttaagagtag atgcatccgt
      541 tcgcgaattt gcggtgggtg atcgcatggg attgaaagtg aaaggaaaag tggttttccc
      601 tgcggagaat gagatgctca tcgctttccc ggaggtggga ttggtgatga tcgaaaatgg
      661 tgggacttcc tttttgtaca agatcgcgga taaaggtctg ctatgtaatg acactttcaa
      721 tgttgtgtat ccatttgcag agaatggaat tgcccaggta ttgctcaata atggcaagga
      781 aggtttcatc gatcgtaatg gtacctatgt agtgatgccg gaatacgatc ggatcgatcg
      841 attgaccaat ggctattggc aggtttttgt gggtgaggat cggagcggga tctgttctcc
      901 ggaatttaaa atgattcttc ctgcggagta tttgcggctc gaacgggaaa aggatattgt
      961 tctggcaaag aaaaaggaag ggatgcaatt gatggatacg ctctatcgct tgctgaatcc
     1021 ggatgtgttc gaggaaattt caacaatgta cgagcatcct tttctcgaaa taaaaaagaa
     1081 tgggaaaaaa ggaatgatga gttacagcgg agaagtactg gtgcctcctg tgtattctga
     1141 tgtttacctt tggaggagcg aatcttttta tacattcaaa tcgggagatt ctacctatac
     1201 gcttcttggt gcagatgggc gaattatcgt acatgaccag ctgagtttcg attacctgaa
     1261 gtttctgcca aatggtgcgg cgatcgcaaa tgttggaggg gcatccatcc tggtatccaa
     1321 atcggagaaa gtattgatcc catgttgcaa ttattggata gacaatttgg tggagaatga
     1381 ttcggttaaa gcagtgcatg ttgaaacggg tgaggcattc aaggcaagta tttataatgg
     1441 tggtcttacc cgtctcaagc gttcttgtct ggatgaatat gaagactata aagtcagtaa
     1501 aatttcagaa aaccaattca ttattccagc aggtgaagat aaatatgtgt atgcagattc
     1561 gttatgcaat ccaaagtcaa aagcatatca ttcgatttat gccaatggaa ttgtaaaact
     1621 aaatgagaag tatggattat tggattcgtc tgccaaggaa gtaattcaac ccatctattt
     1681 gaattatagt tatggaggtc aatacgttgc ttttaaaagt gtggatggat gggagttatt
     1741 tggccgaaac ggaatgaagc tggttccata taagtttgaa gaaattgaat atgttggttt
     1801 agaacattta aatgttaaac aggccggtaa atggggttta ctttccctgg atggaagaaa
     1861 gaattcgatg gttatacctg ctaaatacag tcgaattgat ctgttggaaa ataatgcttt
     1921 ggcggtaagt gatcggggat tgtttggggt ttttgataca acaggtaagg aaattttgcc
     1981 gatgatatac aaggatatca ccgcattaaa aaaagcatat tatgcactgg atcgaccttc
     2041 cgtagtggaa cgaattctgc aattccgcaa ttcaataggt ggtttcagtt ttatggaggg
     2101 aacagatcaa atgttgacct acgatagtga atggatcact ttttgggact tgaaaagtaa
     2161 tactgaaata ggtcatttta ccttgaacaa tacgcctcat ggtgatgcta tgggttacca
     2221 gactattaaa catgcaggct atctcgatcc attaaatatt tatgcagaat tttatatctc
     2281 cggaggggga gggcattatt atagggtact caataccttt gatcaaggag catctatccc
     2341 gcacaatatg tctacgatcg aatttcaact gccgattcct ggctcaggtg gaaaggccgt
     2401 tttggggaaa gatgaaggga ccagggtcga tttgtatcga tatgatccaa tcgatcattc
     2461 ttttaaactc tggcataagg gtgaagaagg gaaggacgtt catacttaca gagtaaatac
     2521 aacacctaat cataacagca cgcattttgg gtattatgat cagatcagta aaaaatattg
     2581 ggttcattcc tttagtaacc cttccctaaa ctatttcttg tctcgtgaaa aatatccaga
     2641 gggatccttt gttttttctg agaagaattt gttgctgtta caagaaggta aatttcaaaa
     2701 attcaatctt acaacaggag ctt
//
LOCUS       DAOMYG010000425         2717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_13992_length_2717_cov_7.588655, whole genome shotgun sequence.
ACCESSION   DAOMYG010000425 DAOMYG010000000
VERSION     DAOMYG010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13992_length_2717_cov_7.588655"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..487)
                     /locus_tag="PK610_12035"
     CDS             complement(32..487)
                     /locus_tag="PK610_12035"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HRJ36713.1"
                     /translation="MKVLMVCLGNICRSPMAEGIMRHKANAVGKSWQIDSAGTSNFHV
                     DEAPDRRSVKKMKEYNIDISSLRGRQFSVSDFEAFDLILAMDESNFSNIMKLAKNDDH
                     RNRVRMILEYTFPGQRMSVPDPYYGGESGFENVYRLLDSACDKILEEHS"
     gene            complement(489..1358)
                     /locus_tag="PK610_12040"
     CDS             complement(489..1358)
                     /locus_tag="PK610_12040"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRJ36714.1"
                     /translation="MLQRAISGLVFVVVLCGAILWSFWSLLALFSLIVVIGLFEFHKL
                     RSTENPNVKKWSILYSGILAFATSVFISMPMQKELGFDFYNHIKTLFFCTICFPGILA
                     IPLMIADMLDENGGGYQNTSNGIFAAFYIGIPFGLLFHLIDFSDSFHYDGRPILAFFI
                     LIWSNDTFAYLSGKFLGKHKLWERISPNKTIEGFLGGLLFTQVAMQIIMRSGMIPELP
                     LAIQIGIPLIVSVFGTLGDLSESLLKRNAGVKDSGQLMPGHGGVLDRFDGILLSLPVL
                     GIFAGIYHLYILL"
     gene            complement(1358..1573)
                     /locus_tag="PK610_12045"
     CDS             complement(1358..1573)
                     /locus_tag="PK610_12045"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRJ36715.1"
                     /translation="MNDPRWVKVYTASNPMDAHIIQAMLDEEEFAVMLLDKQDSSYGF
                     GNVDIYVIEEHAPQAIPVIQKFIENLS"
     gene            complement(1576..>2717)
                     /locus_tag="PK610_12050"
     CDS             complement(1576..>2717)
                     /locus_tag="PK610_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019671353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ36716.1"
                     /translation="RGKSPSLGSNDERENTLNQLLTEMDGFGTNSGVIILAATNRADI
                     LDRALMRPGRFDRMIYVDLPDLNERKKIFAVHLRPLKVAEGLDVDFLARQTPGFSGAD
                     IANICNEAALIAARKKKKFVEKQDFLDAVDRVIGGLEKKNKIISKEEKSVIAYHEAGH
                     AAVSWLLEHASPLVKVTIVPRGQSLGAAWYLPEERQITRTEQMLDEMCATLGGRASEK
                     VVFDKISTGALSDLERVTKTAYAMVSIYGLNDVIGNISYYDPQGGEFAFSKPYSEKRA
                     ETIDTEVSKIIEGQFKRAVELLSANKEKLDKLASLLLEREVIFKEDLEQIFGKRPYDK
                     EEPEEKKPEEKTVSPEGTAEAGEQKNILEWDNLSSGTPPTEPEDK"
BASE COUNT          750 a          604 c          556 g          807 t
ORIGIN      
        1 gtgggaatta aataaagtgt gccttttttc atcaggaatg ctcttcgaga attttatcgc
       61 aggcagaatc cagcagacgg tacacatttt caaatcctga ttcgccaccg tagtaaggat
      121 cgggaacact catgcgctga ccgggaaatg tatattccag aatcatgcgg acacggttgc
      181 ggtgatcatc gttttttgca agtttcatga tgttcgaaaa gttgctctca tccattgcaa
      241 gaataagatc gaatgcttca aaatcactta cgctgaattg tcgtccacgt aatgaagaaa
      301 tatcaatgtt gtattctttc atctttttta ccgaccgccg atcgggtgct tcatcaacgt
      361 ggaaattcga tgttcctgcc gaatcgatct gccaggattt tcccacagca ttggctttgt
      421 ggcgcatgat tccttctgcc attggtgaac ggcaaatgtt tccaaggcaa accattaaga
      481 ctttcatact acaacaaaat atataagtga taaatgcctg caaaaatccc caataccggg
      541 agtgaaagca atattccatc aaaacgatca agcacaccac catgaccagg cataagttga
      601 cctgaatctt ttacaccggc atttcttttt aacaaggatt ctgaaaggtc tcccaatgtt
      661 ccgaaaacgg aaacgatgag cggaattccg atttgtattg caagcggaag ttcgggtatc
      721 attccacttc gcattatgat ctgcattgct acttgggtga atagtaatcc gcctagaaat
      781 ccttctatgg ttttattggg tgaaatacgt tcccataatt tgtgtttgcc aagaaacttt
      841 ccacttagat aagcgaacgt atcgttactc catatgagta taaaaaatgc aaggattggt
      901 ctgccgtcgt aatggaagga gtcggaaaaa tcgatcaaat gaaagagcaa accgaatgga
      961 attccgatat aaaatgcagc aaatattcca ttgctcgtat tttgatatcc tcctccattt
     1021 tcatccagca tatcagcaat cattaatgga atcgctaaaa ttccgggaaa gcagatggtg
     1081 cagaaaaaga gtgtttttat atggttatag aaatcaaatc ccaattcttt ttgcatgggc
     1141 attgaaataa aaacacttgt tgcaaatgcc agtattccgg aatagaggat ggaccacttt
     1201 tttacattag gattttctgt cgatctcagt ttgtgaaatt caaataatcc gattaccaca
     1261 atcaggctga acaatgccaa tagcgaccaa aaactccaaa gaatggcgcc gcataacacc
     1321 acaacaaata cgagtcccga tatcgctctc tgtagcatca ggaaagattt tcaatgaatt
     1381 tctgaatcac aggaatggct tgtggcgcat gttcttcaat gacataaata tccacattac
     1441 cgaagccata gctactgtcc tgtttgtcca gcagcataac tgcaaattct tcctcatcca
     1501 gcatggcctg aataatgtgg gcatccattg ggttggatgc tgtgtacact ttcacccagc
     1561 gcggatcgtt cattcctact tgtcttcagg ttcggttggt ggggtaccgg aagataaatt
     1621 gtcccattcg agaatatttt tctgctcacc ggcttctgca gttccttccg gacttacagt
     1681 tttttcttcc ggttttttct cttcgggttc ttccttatcg tacggacgct ttccaaaaat
     1741 ctgttcgaga tcttccttga agatgacttc gcgttcaagt aacaagctgg caagtttatc
     1801 cagtttctct ttattggcgc tcagtaattc tacggcacgt ttaaactgac cttcaatgat
     1861 cttgctcact tcggtatcga tcgtttctgc gcgtttttcc gaatagggtt tcgagaaagc
     1921 aaattctcct ccttgcggat cgtaatagga aatatttccg atcacatcat tcaaaccgta
     1981 aatggaaacc attgcataag ctgttttggt tacgcgttcc aaatcgctta aagcaccggt
     2041 agagatcttg tcaaaaacaa ctttctccga agcacgtcct ccaagtgtgg cgcacatttc
     2101 atccagcatt tgttcagtac gggtaatctg tcgttcttca ggaaggtacc atgcagcgcc
     2161 taaggattgt ccacgtggca caatggtgac ttttaccagt ggagaggcat gttcaagtaa
     2221 ccaacttact gcggcatgtc ctgcttcgtg ataggcaatt acactttttt cttctttgga
     2281 gatgatttta tttttctttt ccaatccacc gataacacgg tcaactgcat cgaggaaatc
     2341 ttgtttttct acaaacttct ttttcttacg ggcagcgatc aatgcggctt cgttacaaat
     2401 gtttgcaatg tcggcaccgg aaaaaccggg tgtttgacgg gcaaggaaat cgacatccaa
     2461 tccttctgca acttttaggg gacgcaaatg gactgcaaaa attttcttac gctcattcag
     2521 gtcaggcaga tcaacataga tcatcctgtc gaatcgaccg ggacgcataa gtgcacggtc
     2581 aagaatatct gcacggtttg ttgctgcaag tataattaca ccgctgttgg ttccaaaacc
     2641 atccatttcg gtaagcagct ggttaagggt attctcgcgt tcatcgttcg agccgagaga
     2701 aggacttttt ccgcgtg
//
LOCUS       DAOMYG010000426         2708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_14061_length_2708_cov_7.488881, whole genome shotgun sequence.
ACCESSION   DAOMYG010000426 DAOMYG010000000
VERSION     DAOMYG010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14061_length_2708_cov_7.488881"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PK610_12055"
     CDS             complement(<1..542)
                     /locus_tag="PK610_12055"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRJ36717.1"
                     /translation="MNYTDLLNQAEDVTRELIAIVNTSFAPLSYDDLYKHPAPGKWSA
                     AECFEHLNSYHAYYNKAFRLQIEKAKKNPHNRQKTHKSTWLGKWAVRSVHPDAIAKKM
                     NAVKHHNHKGKTITADVLKRYVMNCEEFLELIEASREIDLQKTKVRIEIMKMLRLNLG
                     DFYFFMLRHDQRHVLQASNA"
     gene            complement(615..1229)
                     /locus_tag="PK610_12060"
     CDS             complement(615..1229)
                     /locus_tag="PK610_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRJ36718.1"
                     /translation="MTNNDSGSPFSVMEEQWLITFRSTIEKVSPLKEEEWEGLLSVIQ
                     FRPFSRHDLIISEGEVEHFVSFVCGGVLHGYFLKDGDEHTIAFTYKGHYCASLGSFMS
                     RSPSRYFIQALSDGMLLSIRHADLQQLYDAYRGIERWGRISAEQVLTGFEWRQAELMS
                     FSAEERFRLFMSRSAFLCQLVPQKYIASYLNMTPETFSRLRKQG"
     gene            complement(1240..2334)
                     /locus_tag="PK610_12065"
     CDS             complement(1240..2334)
                     /locus_tag="PK610_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HRJ36719.1"
                     /translation="MKSDKNNYCVIMAGGIGSRLWPMSRTSDPKQFHDVLGAGRTLIQ
                     QTLDRFRDICPIENVLVVTNAQYLDQVKKQLPEIPENNILLEPDRRNTAPCIAYACFR
                     IEAENPNARIVVASSDHLILKADAFRETILMALEQAAAKDCLITLGIKPSRPDTGYGY
                     IQFTDEEGTVSAQVKKVKTFTEKPNLEIAKQFIESGDFYWNSGIFIWSLPSILKALER
                     YMPHTNGLFAEGRGVYATAGEKEFIERTYQQCKNISIDYGVMEKAKNVYVLLSDFGWS
                     DLGTWGSLYTHIRQDDNRNAIVGKQVKTYDTSGCLVNVPKGKLVVLQGLEDFIVVDTG
                     DVLLVCKREEEQRIKEFVSDIKNGKGEKFI"
     gene            complement(2331..>2708)
                     /locus_tag="PK610_12070"
     CDS             complement(2331..>2708)
                     /locus_tag="PK610_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRJ36720.1"
                     /translation="DKKAVDQYLSSFMSELNDRLNGFYDVFSKLKSEGFKVNAIAPQA
                     AIYLTVQFDLVGMKTPAGEVLDSMAKVNRYILDEAKIALVPFYAFGASEDSTWFRLSV
                     GTAQRSDIDAVYVALKSALSKLS"
BASE COUNT          793 a          619 c          508 g          788 t
ORIGIN      
        1 catgcattgg aagcctgaag aacatggcgc tgatcatgcc gcagcataaa aaagtaaaaa
       61 tcgccaagat tcagtcggag cattttcatg atctcaattc tgactttcgt tttctgaagg
      121 tcaatttcgc gcgaagcttc aatcaattcc agaaactctt cacaattcat tacatagcgt
      181 ttcagcacat cagctgtaat cgtttttccc ttgtgattat gatgttttac cgcattcatc
      241 tttttagcaa tcgcatcagg atgaacactg cgaacagccc attttcccaa ccaggtactt
      301 ttatgagttt tctgtctgtt gtgcggattc ttttttgcct tttcaatctg cagtctgaag
      361 gccttattgt aataagcatg ataggaattt aaatgctcaa aacactcagc agcactccat
      421 tttccgggcg ccgggtgttt gtacaaatca tcgtacgaca atggcgcaaa tgaagtattt
      481 acgatagcaa taagttcgcg tgtaacatct tcggcctgat tgagtaaatc tgtgtagttc
      541 atgatcgttc aatttgtttc tgcaaacctg aaccttttgc acaaggaaaa aattgatcta
      601 tatcaatatc gcattcatcc ctgtttacgc agacgggaaa aagtctcggg tgtcatgttg
      661 agataggaag caatatattt ttgaggtaca agctgacaca aaaacgcact tcggctcata
      721 aacaaacgga atcgctcctc tgcagaaaac gacatcaatt ctgcctgacg ccattcgaat
      781 ccggttaata cttgttccgc cgaaatcctt ccccagcgtt caattccacg gtaagcatcg
      841 tacaattgct gcagatcggc atgacgaatg gatagcagca ttccatcgct caaagcctga
      901 atgaagtacc gcgaaggact acgcgacata aacgagccaa gagatgcgca gtaatgtcct
      961 ttataggtaa atgcaatagt atgttcatcc ccgtctttta aaaaataacc gtgaagcaca
     1021 cctccgcaaa caaaacttac aaaatgttca acttctcctt cagaaataat aagatcatgt
     1081 ctggagaaag gtctgaactg aatcacactc aacaatccct cccattcctc ctccttaagc
     1141 ggagaaacct tttctatcgt cgaacggaaa gtgatcaacc actgttcttc catcactgaa
     1201 aagggagatc cggaatcgtt gttggtcatt gcacttcgat taaataaact tttcgccttt
     1261 cccgtttttt atatcgctta caaactcttt gatgcgttgt tcttcttcac gtttgcatac
     1321 caataaaaca tcaccggtat ctacaacaat aaaatcttcg agtccttgca aaaccaccaa
     1381 tttacctttt ggcacattga ccaaacatcc ggaagtatcg tatgttttta cctgtttccc
     1441 aacaatagca ttgcggttgt catcctgacg aatatgtgta taaagcgaac cccaggttcc
     1501 taaatccgac caaccgaaat cagaaagtaa aacatataca ttctttgctt tttccattac
     1561 accgtaatcg atggaaatgt ttttgcattg ctggtaggta cgttctataa attctttttc
     1621 tcctgcagtt gcgtaaacac ctcttccctc agcaaacaaa ccgttggtat gcggcatata
     1681 acgctcaagc gctttaagaa tactgggtaa actccagata aaaatacccg agttccaata
     1741 gaaatcaccg ctttcaataa attgttttgc gatttcaaga ttgggttttt cagtaaaggt
     1801 ttttactttt tttacctgag cagaaactgt tccttcttca tcggtaaatt ggatatatcc
     1861 ataaccagta tccggacgcg agggcttaat accaagcgtg atgaggcaat cttttgcagc
     1921 agcctgttca agcgccatca aaatggtttc gcgaaaagca tctgctttaa gaataaggtg
     1981 atcggaagaa gcgactacaa tacgtgcatt gggattttct gcttcgatgc ggaaacaggc
     2041 ataggcaata cagggagcgg tatttcttcg atccggttcg agcaagatat tgttctccgg
     2101 aatttcaggc aattgttttt ttacctgatc aagatactgc gcattggtta cgacaagcac
     2161 attttcgatc ggacaaatat cccgaaagcg atcgagcgtt tgttgtatta gcgttctgcc
     2221 agcgcccaat acatcgtgaa attgtttagg atcagaagtg cggctcattg gccagagacg
     2281 acttccgatg ccacctgcca ttattacgca gtaattgttc ttatcgctct tcatgatagt
     2341 ttagacaaag cagatttcaa cgcaacatac accgcatcaa tatcggatcg ttgtgctgta
     2401 ccaacagaca agcgaaacca tgttgagtct tctgaagcac caaatgcata aaatggtacc
     2461 aaggcaattt ttgcttcatc aagaatatac cgattcactt tcgccatgga atccaaaact
     2521 tctcctgcag gagttttcat tcccaccaaa tcaaactgaa cggtaagata aatggcagcc
     2581 tgtggtgcaa tggcgttcac tttgaaacct tctgatttca atttactgaa cacatcataa
     2641 aatccattta aacgatcgtt gagttcactc ataaacgaag agaggtactg atcgaccgct
     2701 ttcttgtc
//
LOCUS       DAOMYG010000427         2698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_14143_length_2698_cov_7.352630, whole genome shotgun sequence.
ACCESSION   DAOMYG010000427 DAOMYG010000000
VERSION     DAOMYG010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14143_length_2698_cov_7.352630"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1150)
                     /locus_tag="PK610_12075"
     CDS             complement(170..1150)
                     /locus_tag="PK610_12075"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ36721.1"
                     /translation="MVRSVLLFWIILMSFSASAQRPSSDFVPPCGLPDKGKFLPLIQD
                     SIYYYDSRLRPFPDRGICLEADLICNNGEPLPLIQWLKDNPKTKVEIICHTDHRGDAA
                     SNKRLSQTRADNLRQWLISEGIEASRLVANGKGEDGFLIPEATIKAMKTPEEQNAAHK
                     INRRTEIKILEVNYKFWFGITDQNFFAGQRLDPVLEYEFNKHYDVFFNQQPDKLSHEQ
                     KLQRDSAFRVLNLVGIFLMTHPGIQLEIGVHTDQKDVEANNIVLTRARADAIAKFLFE
                     KGVKKEQLVAKGYGSSKPRNIGANIYNKTKQEQEIMYSENRRVEFKIIKT"
     gene            complement(1144..1797)
                     /gene="hxpB"
                     /locus_tag="PK610_12080"
     CDS             complement(1144..1797)
                     /gene="hxpB"
                     /locus_tag="PK610_12080"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: protein motif:HMM:NF008087.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HRJ36722.1"
                     /translation="MIKAVIFDMDGLLIDSEPIWREAEKEVFATVGISLSDEMCFETV
                     GLRIEEVVDHWHRQFPWSNRSKEEIAIQVVQRVTELIREKGKALPGVQRAIQSLHKEN
                     IPLAIASGSHYSIIHTVVDQLNIRSYFSIIHSAEEEEHGKPHPAIFLSTAKRLNVYPT
                     ECLVFEDSFNGVIAAKAARMKVIAVPEDIHRGEQRFHAADLILETLEDFSPEQLLKW"
     gene            complement(1794..>2698)
                     /locus_tag="PK610_12085"
     CDS             complement(1794..>2698)
                     /locus_tag="PK610_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36723.1"
                     /translation="SDSKGNYFLGDFGNNINKRQNLCVYKLNPFEPKVIAEKIEFSFS
                     DQKLFPPAENNRNFDCEAMFWMDNNLYLFSKDNSKPYSGYTKLYRLSDQPGERTAELL
                     DSMLLGKGGYLEHSVTGAAFDRKNKRMVLLTYQHIYIFYDFEGSDFFKGKLMQFSMGG
                     LRQREAICFDEEGNFFTTEEKRMGIGGMLNFYSLLPWFKGEKLYDRKEVMDVLGRKKE
                     NAIQFSFRCNQPGLYSVEIRGGSSGEFQLLMQTEVKQKENEGKEVLFGKIALPENYDS
                     ETAVLCIYQNSMLVYSRKLIAFNK"
BASE COUNT          720 a          574 c          531 g          873 t
ORIGIN      
        1 ctgaacatat ccgtcatcgt aatggattgt tatacgatta catcagcgcc cgaattgtat
       61 ttagcgagct ggttgtaaag acgtacggcg aaaatacaga cgcttacaat gcgcagatcg
      121 attcggtaaa caataaaatt gaattgctgt tcaatgagct ttctgcacac taagttttaa
      181 taattttgaa ctcaaccctt cggttctcac tatacataat ttcctgttcc tgtttggtct
      241 tattataaat gttcgctcct atatttctgg gttttgaact tccatatcct tttgcgacca
      301 attgttcctt ttttacgcct ttttcgaata ggaattttgc aatggcatcg gctctggcgc
      361 gagtcaatac tatattgttt gcctccacat ctttctggtc ggtgtgtact ccaatctcaa
      421 gttgaatgcc gggatgagtc atcagaaaaa tccctacaag attcaacacc cgaaaagctg
      481 agtcgcgctg aagcttttgt tcatgactta atttgtcggg ttgctgatta aaaaagacat
      541 cgtagtgttt gttgaattca tattccaaca cagggtcgag cctttgtccc gcaaagaaat
      601 tctgatctgt aattccaaac caaaacttgt aattcacttc cagaattttt atttctgtac
      661 gtcggttaat tttgtgtgct gcattttgtt cttccggtgt tttcatggcc ttaatggtcg
      721 cttccggaat cagaaaaccg tcttcacctt ttccgtttgc aacaagacgt gaagcttcaa
      781 tgccttctga aatgagccat tgacgtaaat tatcggcgcg tgtctgggaa agtcttttgt
      841 tagatgccgc gtctccgcga tgatcggtat gacaaatgat ttcgactttg gttttcggat
      901 tgtctttcag ccattgaatt aaaggtaatg gttctccgtt attgcaaatg agatcagctt
      961 ccaggcaaat tcctctgtcg ggaaatggac ggagtctcga atcgtagtaa taaatactat
     1021 cctgtatcag tggtaggaat ttccctttat cgggtaaacc gcagggagga acaaaatcgg
     1081 atgatggtcg ttgtgcactt gctgaaaacg acatcaggat gatccaaaaa agcaaaacac
     1141 ttcttaccat ttcaataatt gttcgggtga gaaatcttcg agagtttcaa gaataagatc
     1201 cgccgcatga aaacgttgtt cgccgcggtg gatgtcttcc ggtacagcaa ttactttcat
     1261 gcgcgcagct ttggcggcaa ttactccgtt aaaggaatct tcaaaaacga gacattctgt
     1321 tgggtaaacg tttaatcgtt tggcagtact gagaaaaatg gccggatgtg gctttccgtg
     1381 ttcttcttct tctgccgaat gaatgatgct gaaatacgat cgaatattta attgatccac
     1441 cacagtatga ataatactgt aatgcgatcc ggatgcaatg gcaagtggaa tgttttcttt
     1501 gtgaagcgat tggattgccc gttgtacacc tggtagtgct tttccctttt cacgtataag
     1561 ttcggtaacc ctttgcacca cctgaatggc aatctcttct ttacttcggt tgctccaagg
     1621 aaattgcctg tgccagtgat caactacttc ttcaatgcgt aatcctacgg tttcaaaaca
     1681 catctcatcg cttaaggaaa tgccaacggt tgcaaagact tccttctcag cttctctcca
     1741 aatcggttcg ctgtcgatga ggagtccgtc catatcaaaa atgaccgcct ttatcatttg
     1801 ttgaatgcaa tgagttttct tgaatatacc agcattgaat tttgataaat acacaaaaca
     1861 gctgtttcgg aatcataatt ttcaggtaat gcaatctttc caaacaacac ttctttccct
     1921 tcgttttctt tttgttttac ttctgtctgc attaacaact gaaattctcc tgaactgcct
     1981 ccgcgtattt caaccgaata caaaccgggt tgattgcagc gaaaactaaa ttgaatggcg
     2041 ttttcttttt ttctacccag cacatccatt acttctttac gatcgtataa tttttcaccc
     2101 ttaaaccagg ggagaagcga gtaaaaatta agcatgccgc ctattcccat tcgtttttct
     2161 tctgtcgtaa aaaagtttcc ttcttcatca aaacaaatgg cttcgcgctg acgaagtcct
     2221 cccattgaaa actgcattag ctttccctta aaaaaatcgg agccttcaaa atcatagaaa
     2281 atataaatgt gctggtaggt gaggagtacc attcgcttat tcttacgatc gaatgctgcc
     2341 cctgttaccg aatgttcaag gtatccgcct ttacccaata acatactatc caataattct
     2401 gccgtacgtt ctccgggctg atcactcagg cggtagagtt tggtgtatcc ggaatacggt
     2461 ttactgttgt ctttggaaaa gagatataga ttgttgtcca tccaaaacat cgcttcgcaa
     2521 tcgaaattgc ggttgttctc tgccggagga aaaagtttct ggtcgctaaa ggagaattca
     2581 attttttccg caatgacttt gggttcaaat ggattcagtt tgtaaacgca gagattttga
     2641 cgtttattga tgttattgcc gaaatcacca agaaaataat ttccttttga atcactgc
//
LOCUS       DAOMYG010000428         2694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_14178_length_2694_cov_8.333839, whole genome shotgun sequence.
ACCESSION   DAOMYG010000428 DAOMYG010000000
VERSION     DAOMYG010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14178_length_2694_cov_8.333839"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1755)
                     /locus_tag="PK610_12090"
     CDS             complement(<1..1755)
                     /locus_tag="PK610_12090"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRJ36724.1"
                     /translation="MENSEFAQEDVILGTVNPFRLLILLLVWLICLPCSLGAQRVGLV
                     LSGGGATGMAHIGVLKALEENGIPIDYITGTSAGALVGGMYAAGYSPAEIEAMVTSEK
                     FLRMSEGRIEPQYIYYFKQFDPSSGMVSLRLSKDSLKQTYLPTNLVSPTLMDYEMMTG
                     FSSAAAAAGYNFDSLFVPFRCVASDIVDKKSVVFANGQLHTAVRASMSFPFYIKPLKV
                     NGRLLFDGGLYNNFPADVMYDVFLPDIMIGSNVSGNTPLPDEDNIMSQIRNMIVTLQD
                     FELRCDNGIIIQPQSGISTFDFASAREAIDAGYRETIKNIDSIRKVVGRTVDPAEIKA
                     RREAFRKKCPPLEFDQIEVEGITKNQQQFVQRTLVKRKEKSISEQKLKPRYFRVGNDD
                     HIAGIYPTAIYNPATGKYNLKLDIKKEKEFNVEFGGNFSSRPINTGYVGLQAHFLGRS
                     AWTVTGRSWFGKFYAAGYGSIRYEPPTKLPFYIEPEFTLQRRNYFKSFSTFFEDVKPS
                     YIIQTEQQVGLNLGFPVGNRGKLVVNGKYAELIDDYYQTQSFLSIDTSDQTKLYAASP
                     SVYYEINTLNKKQYANEGM"
     gene            1782..>2694
                     /locus_tag="PK610_12095"
     CDS             1782..>2694
                     /locus_tag="PK610_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36725.1"
                     /translation="MTGKAFRIIFASVLLFLNAFIYSVAFSPNEPAHLHYIPADAQAV
                     ICMNSRSIAGTLLYNVFFHEEQIVSLMEGDDESEKIDWTANMDNGLQFLGRITFVIMQ
                     APSGEQLSFMMADVAKESKLEKFLSKREGTAEDLDQAVHFHSDGSVSFAYNNEVALIF
                     TGSKDLAAIKKTALHVLHQKNPSPRDWKTIAGEEDFMMELHPGIEKMDDAPALKGLLG
                     SFISSLRFNGVFGEDGLDMKYVLSTDPSLLANASDVFVLHEHQKEIPVDLLDGIFNLH
                     LTFDPDRWVDWIAKGDLLDVPDSLESKL"
BASE COUNT          714 a          558 c          623 g          799 t
ORIGIN      
        1 catgccttcg ttggcatact gttttttatt aagcgtattg atttcataat aaacactggg
       61 tgaagcagca tacagttttg tttggtcaga agtatcgatg gaaagaaaac tttgcgtttg
      121 gtagtaatca tcaatgagtt cagcgtattt tccatttaca accaattttc cgcggttgcc
      181 aacaggaaat cccagattca atccgacctg ttgttcggtc tgaataatat atgatggttt
      241 tacatcttcg aaaaaggtgg aaaaactttt gaaataattc cttcgctgca aagtgaattc
      301 aggttcgata taaaagggaa gtttcgtcgg tggttcgtag cgaatagatc cataacctgc
      361 cgcataaaat tttccaaacc aagatctacc cgtaactgtc cacgccgatc gaccgaggaa
      421 atgagcctgg agtccgacat acccggtatt gattggtcgc gatgagaaat tgcctccgaa
      481 ttctacattg aattcttttt cttttttgat gtcgagtttc agattgtatt ttcccgtagc
      541 gggattgtaa atggcagtag gataaattcc tgcaatgtga tcatcgttcc cgactctgaa
      601 atagcggggt tttagttttt gctcgctgat gcttttttct tttcgcttta caagtgttcg
      661 ctgtacaaac tgttgctggt ttttggttat gccttctact tctatctggt caaactcaag
      721 tggcggacat ttttttctga atgcttccct tcgcgctttt atttctgcag gatcaacagt
      781 tctgccgaca acttttcgaa tggagtcgat gtttttaatc gtttcccggt atcctgcatc
      841 aatagcttcg cgtgctgatg caaaatcgaa agtggaaatt ccggattgag gttgaataat
      901 tattccatta tcacaacgta gttcaaaatc ctgcagggtt acgatcatgt tgcgtatctg
      961 cgacatgata ttatcttcat cgggtagcgg cgtgtttcct gaaacattcg aaccaatcat
     1021 aatatccgga agaaaaacat cgtacatcac gtcagcagga aaattgttgt aaagaccgcc
     1081 atcaaagagt aaacgtccgt tcactttaag cggtttgatg taaaagggaa aggacatgga
     1141 tgcgcggaca gcagtgtgta gttgtccgtt tgcaaagact accgattttt tgtcgacaat
     1201 atcactggcc acacagcgga agggaacaaa cagactatcg aaattataac ccgcagctgc
     1261 agccgcagag gaaaatccgg tcatcatttc ataatccatc agtgtaggtg ataccaggtt
     1321 ggtgggcagg taggtctgtt tgagtgaatc tttggagagg cgtagagaga ccattcccga
     1381 tgagggatca aattgcttga aatagtaaat gtattgtggc tcgatacgtc cttccgacat
     1441 ccggaggaat ttttcggagg tgaccattgc ttcaatttca gcgggagaat agccggcagc
     1501 atacattccc ccaaccaatg cacctgcaga ggtcccggtg atataatcga ttggaatccc
     1561 gttttcttcc agtgctttaa gtactccaat atgcgccatc ccggtagctc caccgccgga
     1621 aaggaccagt cctactctct gagcgccaag cgaacacggc aggcagatga gccagacaag
     1681 aagtaaaatg aggagccgaa atggattcac agtgcccaaa attacgtctt cctgagcgaa
     1741 ttcgctattt tccattattt tagccctaaa attttacaac aatgacaggt aaagcattcc
     1801 ggatcatctt cgcttcagta cttcttttcc tgaatgcttt tatttattcg gtggcttttt
     1861 cgcccaatga acctgctcat ttgcattaca ttcctgccga tgcccaggcg gtcatttgta
     1921 tgaattcgcg gagtattgca gggacattgt tgtacaacgt tttctttcac gaagagcaga
     1981 ttgtatcgct gatggaaggc gatgatgagt cggagaaaat cgactggact gcaaatatgg
     2041 ataacggtct gcagtttctg ggtcggatta catttgtgat catgcaggca ccaagtggtg
     2101 agcagctttc atttatgatg gccgatgttg ccaaggagag taaactggaa aaattcctca
     2161 gcaagcgtga gggaacagct gaagatctcg atcaggcagt acattttcac agcgatggtt
     2221 cagtttcctt cgcttacaac aatgaagtag cgctgatctt taccggatca aaagatctgg
     2281 cagcaatcaa aaaaacagcg ttgcatgtgt tgcatcagaa aaatccatct cctcgcgatt
     2341 ggaagaccat tgccggtgaa gaagatttta tgatggaatt gcatccggga attgaaaaga
     2401 tggatgatgc accggcgctg aaaggattgc tcggaagttt tatttcttcg cttcgtttca
     2461 atggcgtttt cggtgaggac ggactcgata tgaagtatgt gctttcaacc gatccttctc
     2521 ttttggccaa tgcttcggat gtgtttgttt tgcatgagca ccagaaagaa attcctgttg
     2581 atctgctcga tggaatattt aacctgcatc ttaccttcga tcccgatcgt tgggtggact
     2641 ggatcgcaaa aggtgatttg cttgatgttc ccgattctct tgaaagtaaa ctgt
//
LOCUS       DAOMYG010000429         2686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_14251_length_2686_cov_8.224249, whole genome shotgun sequence.
ACCESSION   DAOMYG010000429 DAOMYG010000000
VERSION     DAOMYG010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14251_length_2686_cov_8.224249"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..918)
                     /gene="sufC"
                     /locus_tag="PK610_12100"
     CDS             complement(160..918)
                     /gene="sufC"
                     /locus_tag="PK610_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRJ36726.1"
                     /translation="MISIQNLHANIEGKEILRGINLEIKAGEVHAIMGPNGSGKSTLA
                     SVLAGREEYEVTEGKVIFNAKDLLELSPEDRAREGLFLAFQYPVEIPGVSNVNFLKTA
                     VNEIRAYKGMQPVSAKEFLQMVKDRSALVELDSKLASRSVNEGFSGGEKKRNEIFQMA
                     MLEPKFAILDETDSGLDIDALRIVANGVNKLKNKDNATLVITHYQRLLDYIVPDFVHV
                     LYKGKIVKTGSKDLALELEEKGYDWIKAELGETV"
     gene            complement(934..2382)
                     /gene="sufB"
                     /locus_tag="PK610_12105"
     CDS             complement(934..2382)
                     /gene="sufB"
                     /locus_tag="PK610_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRJ36727.1"
                     /translation="MAYEGDELLDEITNSEYKYGFVTNIESEMAPKGLNEDIIRFISA
                     KKNEPEWLLTWRLNAFRQWQKMTEPSWAHVDYVPVDYQDIHYYSAPKPKKELSSLDEV
                     DPELLKTFEKLGISLDEQKRLTGVAVDVVMDSVSVKTTFKEELAKHGIIFCSMSEAVQ
                     EHPELVRKYLGTVVPVSDNFFAALNSAVFTDGSFCYIPKGVRCPMELSTYFRINEAKT
                     GQFERTLVIADESSYVSYLEGCTAPIRDEHQLHAAVVELVALEKAEIKYSTVQNWYPG
                     DKDGKGGIYNFVTKRGICLGDHSKISWTQVETGSAITWKYPSCILKGNNSIGEFYSVA
                     VTNNKQQADTGTKMIHLGKNTKSTIVSKGISAGYSNNSYRGLVKIAKGATNARNFSQC
                     DSLLMSDKCGAHTFPYIEINDKSAKVEHEATTSKIGEDQIFYCNQRGIETEKAISLIV
                     NGYCKEVLNQLPMEFAVEAQKLLAVSLEGSVG"
     gene            complement(2395..2676)
                     /locus_tag="PK610_12110"
     CDS             complement(2395..2676)
                     /locus_tag="PK610_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRJ36728.1"
                     /translation="MQEESDKKNAFIRVGVKGGGCSGLMYELSFDNEMGPDDKVFEDN
                     GIQVVVDKKSFLYLVGTELDFSGGLNGKGFVFRNPNADRTCGCGESFSI"
BASE COUNT          692 a          641 c          492 g          861 t
ORIGIN      
        1 ggtatacttc cagtcctcct cccgctgagt tgggaaatta agataggatg catttccaac
       61 cagcgttccc cgagattgct ttaactgttc gggaaattga gcctttatcg attctacaaa
      121 tgcagatgcg gttgcttgtt gtgtagtttc catctgcgat taaactgttt ctcccaattc
      181 ggctttaatc cagtcgtacc ccttttcttc gagttcaagg gcgagatctt tgctgccagt
      241 ctttacaatt tttcctttgt acaaaacatg aacaaaatcc ggtacgatat aatcgagcaa
      301 gcgttggtag tgagtaatta ctaaggttgc gttgtctttg ttttttaatt tatttacacc
      361 attggcaaca atccggagcg cgtcaatatc caatcctgaa tccgtctcat cgagaattgc
      421 gaatttgggt tcaagcatgg ccatttggaa aatttcattt ctctttttct ctcctccgga
      481 gaatccttcg tttacagagc ggctggcgag tttgctgtcc agttcaacaa gcgcagaacg
      541 gtctttcacc atttgcaaaa actctttagc tgaaacaggc tgcatccctt tataggcgcg
      601 aatctcgtta acggcggttt tgaggaaatt gacattgctc actcccggaa tttccaccgg
      661 atactgaaac gccagaaaca atccttctcg tgcgcgatct tcgggagata actcgagtag
      721 atcctttgcg ttaaaaatta cttttccttc tgttacttca tactcttctc ttccagcgag
      781 tacagatgca agtgtggatt ttccggatcc gttgggtccc ataatggcat gcacttcgcc
      841 ggctttgatt tcgagattga ttccgcgaag aatctctttc ccttcgatat ttgcatgcag
      901 attttgaata ctgatcatct tctttttcgt tttttatcca acacttcctt ccaatgaaac
      961 cgcaagtaat ttctgggctt ccactgcaaa ttccatgggt aattgattta gcacttcttt
     1021 acaatatcca ttcacgatca aactaatcgc cttttctgtt tctattcccc gttgattgca
     1081 ataaaaaatc tgatcctcac caatttttga tgttgtggct tcgtgttcaa cttttgcaga
     1141 tttgtcgttg atctcgatgt acggaaatgt atgtgctcca catttatcac tcatcagcaa
     1201 cgaatcgcac tgtgagaagt ttcgtgcatt ggtggctccc ttggcaattt ttaccaaacc
     1261 tcggtaactg ttgttggagt atccggccga aatacctttt gaaacaatgg tgcttttggt
     1321 gtttttcccc aggtgaatca tttttgttcc ggtatccgcc tgctgcttgt tgttggtcac
     1381 tgcaaccgaa taaaattcac caatggaatt attaccctta agtatacacg acggatattt
     1441 ccaggtaatt gcagaaccgg tttcaacttg tgtccaactg atctttgaat gatctcccaa
     1501 acaaattccg cgttttgtca cgaaattata aataccgccc ttaccgtctt tgtcaccagg
     1561 ataccagttc tgtacagtag aatattttat ctctgccttt tcaagtgcaa caagttctac
     1621 aaccgcagcg tgcaattggt gttcatcacg aattggtgct gtacaacctt cgagataact
     1681 cacataactg gattcatcag caattacaag ggtgcgttca aactgtcctg tttttgcttc
     1741 gttgattcgg aagtaggtcg ataattccat tggacaacga actccttttg gaatataaca
     1801 gaacgaaccg tcagtaaaca ctgctgaatt caatgcggca aaaaaattgt cactcacagg
     1861 aacaacagtg cccagatatt tgcgaaccaa ctccggatgt tcctgaacgg cttccgacat
     1921 cgaacagaaa atgattccat gtttagcaag ctcttccttg aatgttgttt ttacggaaac
     1981 gctatccatc actacatcta ccgccactcc ggtcaagcgt ttttgctcat ccagtgagat
     2041 acctaacttt tcaaacgtct tgagtaattc aggatctact tcatccaaac tagacaattc
     2101 ctttttcggt ttcggagcgg aataatagtg gatatcctga taatcgacag gaacataatc
     2161 gacatgtgcc caggaaggtt ctgtcatttt ttgccattgc cgaaacgcat tcaatcgcca
     2221 ggtcagtaac cattccggtt cattcttttt tgcagaaata aaacgtatga tatcctcgtt
     2281 cagccccttt ggagccattt ccgattcaat gttcgtaacg aatccgtatt tgtattcgct
     2341 attggtgatc tcatctaata attcatctcc ttcgtatgcc attatacttc gctgttaaat
     2401 agaaaatgat tcgccgcatc cacaagtacg atccgcattc ggattacgaa atacaaagcc
     2461 cttaccgttt aatccaccag aaaaatccaa ctccgttcca acgaggtaaa gaaaactctt
     2521 cttgtcgacc accacctgaa ttccattgtc ttcaaatact ttatcgtctg gacccatttc
     2581 attatcgaaa ctgagttcat acatcaatcc ggaacagcct ccgcctttaa ctcctacccg
     2641 gataaaagca ttttttttat cggactcctc ctgcatcaat ttgatg
//
LOCUS       DAOMYG010000430         2678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_14301_length_2678_cov_6.437286, whole genome shotgun sequence.
ACCESSION   DAOMYG010000430 DAOMYG010000000
VERSION     DAOMYG010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14301_length_2678_cov_6.437286"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..306
                     /locus_tag="PK610_12115"
     CDS             1..306
                     /locus_tag="PK610_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013304440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HRJ36729.1"
                     /translation="MSEMEQQLNIELTEETAEGVYSNLAIITHSNAEFIIDFVRVMPG
                     VPKAKVKSRIVLTPIHAKQLFRALGDNLSKFEQMHGPIKGADVTPIPMNFGGTNTQA"
     gene            complement(307..726)
                     /locus_tag="PK610_12120"
     CDS             complement(307..726)
                     /locus_tag="PK610_12120"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRJ36730.1"
                     /translation="MEMQQIIDEYIKANHFGRLLNMEFQIQEPGKIIYTLPSRKELLA
                     TETAMHGGALAAFMDAILGVAALSAVAGEGKRVATLELKISFLQAVPENDLLTGYGTV
                     LKKGNRIVFSEGEIRNQNNEIIAKGSGTFTAYPIAKS"
     gene            782..1612
                     /locus_tag="PK610_12125"
     CDS             782..1612
                     /locus_tag="PK610_12125"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HRJ36731.1"
                     /translation="MKRCIALLFIAFLAVSCSKKEKFLLRFQPEKGSMKTMVMNLTTK
                     MGGMPMETKMGMTTEMKIANVSPEGEVEVEATYTHVMMNVDAMGRSIGFDSAKDTIDM
                     NDPSSVAYLGLAALLNKPLAMKMDKLGKLTLAPDILSVYPDSLRSLLGSQGSSQGSQM
                     FDNMFSVFPEEEIGLGDSWERESKIGTASGPVSMKATYTVDKVNESDVLLKMEGTFEG
                     EMAGEMGASIKMTGTMTGTLTIDRKTGWTVSTDMVQKIDMNTMGMKMEATNTISITTK
                     "
     gene            1894..2604
                     /locus_tag="PK610_12130"
     CDS             1894..2604
                     /locus_tag="PK610_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRJ36732.1"
                     /translation="MKTVLHKAATRGHANHGWLDSHHTFSFANYYNPERMRFGVLRVL
                     NDDVVDGGMGFGTHPHDNMEIISIPISGDLEHKDSMGNTMVIRQNDVQIMSAGTGITH
                     SEYNKNKDKKVNFLQIWVFPKERQIKPAYGQQQFLPATQQNKLIQVVSPAEDKDGLTI
                     LQDAWFSLGRFDAGKSFDYSVKRKGNGAYVFVIEGELKIGDQALSRRDGFGIWETEKF
                     SVEITGDAHVLIMDVPMN"
BASE COUNT          798 a          508 c          603 g          769 t
ORIGIN      
        1 atgagtgaaa tggaacaaca gctcaatatt gagcttactg aagaaaccgc agaaggtgtt
       61 tattctaatc ttgcgattat tacccattcc aacgccgaat tcatcatcga ttttgtgcgg
      121 gttatgcctg gggtgccaaa ggcaaaagtg aaatcacgca tcgttctgac gcccattcac
      181 gctaagcaat tgtttcgtgc acttggcgat aatttgtcga agtttgaaca gatgcatgga
      241 ccaataaaag gtgccgatgt tactcccata ccgatgaatt tcggggggac aaatacgcaa
      301 gcctgatcag gatttagcaa tggggtaggc ggtaaatgtt ccgcttccct ttgctatgat
      361 ttcattgttt tgattcctga tttctccttc tgagaacaca atacgattcc ctttttttaa
      421 aacggttcca tatccggtaa ggagatcatt ttctggtaca gcttgcagaa aggagatctt
      481 caattcgagt gttgcaaccc gttttccttc ccctgcaact gcgctcaaag ctgctacacc
      541 caaaatggca tccataaatg cagcaagcgc gcctccatgc attgctgttt cagtggccag
      601 caattctttt cgggatggca gagtgtaaat aatctttcct ggttcctgaa tttgaaattc
      661 catgttcaga agtcgaccaa aatgattcgc tttgatatat tcatcaatta tttgctgcat
      721 ttccatgtgg taaaattacc gattcggatc cttttcccta attttattca aaatctaaac
      781 tatgaaaaga tgtattgcat tgcttttcat cgcatttctg gcggtgtcct gctctaaaaa
      841 agaaaaattc ctgctccgtt ttcaacccga aaaaggaagc atgaaaacca tggtaatgaa
      901 cctcactaca aaaatgggtg gcatgccaat ggaaactaaa atggggatga ccacagaaat
      961 gaaaatcgcc aatgtttctc cagaggggga agttgaagta gaagcaacct acacgcatgt
     1021 gatgatgaat gttgacgcaa tgggtcgttc aatcggattt gactctgcaa aggacaccat
     1081 cgatatgaat gatccttctt ccgtggcgta tttgggtctt gccgcattgt tgaacaagcc
     1141 tcttgccatg aaaatggata agctgggtaa acttactctt gctcccgata tcctcagtgt
     1201 atatcccgac agcttgcgaa gcttattagg tagtcagggt agctcacagg gaagtcagat
     1261 gttcgataat atgttttctg tatttccgga agaagaaatc ggattgggcg acagttggga
     1321 acgcgaaagc aaaatcggaa cagcttccgg tcctgtaagc atgaaggcaa cttatacagt
     1381 agacaaggtg aatgagagtg atgtcttgtt aaagatggaa ggcacttttg aaggtgaaat
     1441 ggccggtgaa atgggcgcaa gcattaaaat gacaggaacc atgaccggaa cacttaccat
     1501 cgatcgcaaa acgggatgga ccgttagcac ggatatggtt cagaaaatcg acatgaacac
     1561 catgggaatg aagatggaag ctaccaatac gatttccatc actacgaagt aatcgattga
     1621 taaacaaata gtaaaggctc cgtttttgcg gggctttttt tgtgactgat tttttatact
     1681 taggtaattg ttgtatatac aattatttgt tgtatctttg tattgtcaaa cgaatcaacc
     1741 atgaaacgaa aagaattttt acaacgcgga ttaggtggtg tttttggact aattgcattg
     1801 ccttccgttt ttgcaaaatc atcggaagat gaacatgagc agaaagttga acaagtcgga
     1861 tttaatcatt tacccaataa ggagatcaaa actatgaaaa cggtacttca caaagcagca
     1921 acaagaggac atgcaaatca cggctggctt gattcgcatc acacattcag ttttgcgaat
     1981 tattataatc cggagagaat gcgttttggg gttttgcgcg tgctgaatga tgatgttgta
     2041 gatggtggaa tgggatttgg aacgcatccg catgataata tggaaatcat ttccattccg
     2101 atttcaggtg atttggagca taaagacagt atggggaata caatggtcat tcgtcagaac
     2161 gatgtgcaga tcatgagtgc aggaaccgga attacacact ccgaatacaa taagaacaag
     2221 gataaaaaag tcaattttct tcaaatctgg gttttcccca aggaacgtca gattaaacct
     2281 gcatacgggc aacaacaatt tcttccagcc actcagcaga ataaattgat acaagttgtt
     2341 tctcctgccg aggataaaga tggcttaact attctccagg atgcatggtt ttcattgggg
     2401 agatttgatg ccggaaagtc atttgattat tccgttaaac gcaaaggaaa cggagcgtat
     2461 gttttcgtta ttgaaggcga actgaaaatc ggtgatcagg ctttatcgcg tcgtgatggt
     2521 tttggtattt gggaaacgga aaagttttcg gtggaaatca ccggtgatgc tcatgttttg
     2581 attatggatg tcccaatgaa ctaagtatga aaatcaaaaa caaagcgctg ttaccatttc
     2641 tatttgtatt tcttgtcgca actcctttaa gtttttgc
//
LOCUS       DAOMYG010000431         2668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_14367_length_2668_cov_7.994642, whole genome shotgun sequence.
ACCESSION   DAOMYG010000431 DAOMYG010000000
VERSION     DAOMYG010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14367_length_2668_cov_7.994642"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="PK610_12135"
     CDS             complement(<1..1141)
                     /locus_tag="PK610_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36733.1"
                     /translation="MILCPIFVFAQETEEEEETLPEDQEGFIFRPTIGLGVGTFTFMG
                     DVGLYNKGYHPTVSRMGYNLTVFNPLTPEIGLNFYAIFGTVSSSERSLTRNLNFQSQI
                     RTGGMVLSYNFGHFLPEKRFLEPYVFAGIESFEFLTKTDLYDRFGNRYNYWSDGSIRN
                     IAENDPNAATAMIIHRDYHYETDVRESNVDGFGKYPERSWAVPVGIGFSMNLNRRWTL
                     KMSTSMHFAFTDLVDGVSDASLGPRQGDGKNDRFLYTTTTLTYDLQRLGSSKKKKEDG
                     MIDEEMEDYLARDTVDTDRDGIPDFVDDCAWTPKGVEIDKRGCPVDKDKDLVFDYLDD
                     ELPTPKGNLVDLKGVTVTDEDLYERWLRYSDTTGKYVVFETIREDG"
     gene            complement(1213..2238)
                     /locus_tag="PK610_12140"
     CDS             complement(1213..2238)
                     /locus_tag="PK610_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36734.1"
                     /translation="MRSWTLSVLVGLLSAGTFLLFCSLLPAPEQISLELTIPGSILNE
                     EEKEFTLTLNKGTLIGPARINIEIRKSGIDINAIELSGAELLKEGDIHSIVWTELPAS
                     PAINLKLRLKGHRFFTGIFTVLVSFSYIEEGSSREKELARKELRVISSGKSQSTASTG
                     TKTGTTTSTSGGTSNTTTTGTTTTGSTTTGSTTTNENSTGTTSGNTGTSNTTTSTSTG
                     NTGTTGTVTENKTMIDPITKKTGTPIRDASTIPGLFFRVQVAASHFLVTQEYFTDRYK
                     FDEQLYVDNVDGWVKYTVGDFKTVEEALAKKATLVSFPFKGPFLVAFNDGKRIPIYSA
                     RQLSTFK"
     gene            complement(2319..>2668)
                     /locus_tag="PK610_12145"
     CDS             complement(2319..>2668)
                     /locus_tag="PK610_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRJ36735.1"
                     /translation="DFDKLITFDYFFVRKVMFVKFSQGFIVLPGGFGTLDELFEALTL
                     IQTKKIGKFPVVMVGKEYWSGLVDWIKNTMLDKEKNISPEDLNLWQVVDTPEDAVKAI
                     EDFYNKFMLKPNF"
BASE COUNT          678 a          581 c          605 g          804 t
ORIGIN      
        1 aaccgtcctc gcgaatggtt tcaaatacaa catattttcc tgtagtatcc gaataccgca
       61 accagcgttc gtaaagatct tcgtcggtaa cagtaacgcc tttcagatcg accagatttc
      121 cttttggtgt gggtaattca tcatcgagat aatcgaaaac aaggtctttg tctttgtcaa
      181 ccggacaacc gcgtttgtcg atctcaacgc ctttgggagt ccatgcgcaa tcatcaacga
      241 aatcgggaat gccgtctctg tcggtatcaa cggtatctct tgcgagatag tcttccatct
      301 cctcatcgat cattccgtct tcttttttct ttttggaact tcccagtcgt tgtaaatcgt
      361 aagtgagggt ggtagtagtg tacaggaaac gatcgttctt tccatcacct tgtcgcggtc
      421 ccaggctggc atcgctaact ccatctacaa ggtcggtgaa agcaaaatgc atactggtac
      481 tcattttgag tgtccatcgg cgattgaggt tcatcgaaaa tccaattccc actggtactg
      541 cccaggagcg ttcggggtat tttccaaatc cgtcaacgtt cgattcgcgc acatcggttt
      601 cataatggta atcgcggtga atgatcattg ctgttgcggc attgggatca ttttctgcaa
      661 tgttccgtat cgaaccatcg ctccaatagt tataacggtt accaaaacga tcgtacaaat
      721 ccgttttcgt tagaaattcg aacgattcaa tccctgcaaa tacatagggt tccagaaatc
      781 gtttttcagg taaaaagtgc ccgaaattgt aagacaaaac cattccgcct gttctgatct
      841 gcgattgaaa attcagattc cgggtgagcg atcgttcgct cgaagaaacc gttccgaaaa
      901 tggcatagaa attcaaaccg atttcgggag taaggggatt aaaaacggtg agattgtaac
      961 ccatgcgact cacggttggg tgatatcctt tgttgtacaa gccaacatct cccataaagg
     1021 tgaacgtgcc gacacctaat ccaatagtgg gacggaaaat aaatccttcc tgatcttcag
     1081 gtaatgtttc ctcttcttcc tccgtttcct gcgcgaaaac gaagattgga cagagaatca
     1141 ataccaggaa aaagatatgt ctgtttaacc tgcacatttg catgtctaag gtaggcaaaa
     1201 gacctgaaaa aatcacttga atgtgctcag ttgtcgggcg gagtagatgg ggattcgctt
     1261 tccatcgtta aaagccacca ggaaagggcc tttgaaaggg aatgaaacca gcgtggcttt
     1321 tttggcaagg gcttcttcta cagtcttgaa atcgcctaca gtatacttca cccagccatc
     1381 aacattatca acatacagct gctcatcgaa tttgtaccgg tcggtaaaat attcctgcgt
     1441 taccaaaaag tgactcgcgg caacttgcac acggaagaag agtccgggaa tggtggaagc
     1501 atcacgaata ggtgtccctg ttttcttcgt gatcggatcg atcatggttt tgttttcagt
     1561 cactgtacct gttgtgcctg tgtttcctgt agaagttgaa gttgttgtgt tgcttgttcc
     1621 ggtatttccg cttgttgtac cggttgagtt ttcatttgtg gttgtgcttc ctgttgttgt
     1681 acttcctgtg gttgtagtac ctgttgtagt ggtgttgctt gttcctccgc ttgtagatgt
     1741 agttgttcct gttttagttc ctgttgaggc ggtgctctgg cttttccctg aggagattac
     1801 gcgaagttct tttctggcca gctccttttc acggctgctt ccttcttcga tataggaaaa
     1861 gcttacaagg actgtaaata ttccggtaaa aaaacggtga cctttcagtc gtagtttgag
     1921 gttgatagcg ggtgatgcgg gtagctctgt ccaaacaatt gaatgaatat ctccttcttt
     1981 gagtaattct gcgcctgata attcaatggc gttgatgtca attcctgatt tacggatttc
     2041 aatgttaatg cgtgccggtc cgatgagtgt tcccttgttg agggtaagtg taaattcttt
     2101 ttcttcctcg ttcagaattg atccggggat ggtaagttca agcgaaattt gttcaggagc
     2161 ggggaggagt gaacagaaaa gcagaaaagt ccctgcagac aacagaccta ccaatacaga
     2221 aagtgtccaa cttctcattc acccaaatct aatctatagc aaggctaaat gagaggtgta
     2281 ggggagtggg ttatgcacag ctcgatgttg aacagtgctt agaaattcgg tttcagcatg
     2341 aatttattgt agaaatcctc aatggccttc accgcatctt ccggtgtgtc taccacttgc
     2401 cataggttga ggtcttccgg agaaatgttc ttttccttat caagcatcgt attcttgatc
     2461 caatctacca gtccgctcca gtattctttt ccgaccatta ccaccgggaa cttcccgatt
     2521 tttttggttt ggattagcgt aagagcttca aacaattcat caagagtgcc aaatcctccg
     2581 ggcaacacaa tgaagccttg tgagaatttc acaaacatga ctttccttac aaaaaagtaa
     2641 tcgaaggtga tgagtttgtc gaaatcaa
//
LOCUS       DAOMYG010000432         2654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_14487_length_2654_cov_6.799923, whole genome shotgun sequence.
ACCESSION   DAOMYG010000432 DAOMYG010000000
VERSION     DAOMYG010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14487_length_2654_cov_6.799923"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..2257)
                     /locus_tag="PK610_12150"
     CDS             complement(215..2257)
                     /locus_tag="PK610_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ36736.1"
                     /translation="MTIVNVENSIVSAGNTALEVLRRSPGVMIDKDGNISLKGKSGLT
                     IMIDGKPTYLSSQDVSTMLSNLPADQVERIEIITNPSARYDASGQGGVINIVLKKNKM
                     LGSNGSVYVSYTQGFYDRENAGFNLNHRTEKWNFFGSYNYGRRNSANWFDLSRKFREN
                     EQVESIFKQTSQGKDTSQSHSGKFGVDFYPHKNHTIGFFVNAMQTTGGDRNTNNTFIT
                     DAIGGPLSSSQTNGKNSEKWSNFNSNINYRWVTDTTGGELKVNLDYAIFDQSNPQFFV
                     TDYFDAGGTPFGIPLILRSELPGKVDIKSAKADYVKSINKKLRLETGIKSSFVSTDNN
                     ARYWIVENGTEYVDTTKTNHFKYTENINAAYLNGQYQLSDKIGLQFGLRVENTRARGE
                     QITIDSVFTRNYTQLFPSGAMSYTINKKNEINFSYSRRIDRPSYRDLNPFLYFLDPYT
                     YMRGNTLLQPQFTNAFEVGHTFMGFLTTTVGYSHTTGVITQVTSQVDSTRTTFATNEN
                     LNSLENWTISSMLPIPISKWWMSMNFITAYHNQYNGKISGQSFDVGMWSWMINSTNQF
                     TFSKGWSAELSAFYMSKQVYGVFIMKPMSSISLGVQKKIMKEKGTIKFSAGDLFWKSR
                     FRGTVFFNNMDINMEAWSDSRVFTLSFSYKFGNAKIQNQRKESGAKDELDRIKQGN"
     gene            complement(2306..2566)
                     /locus_tag="PK610_12155"
                     /pseudo
     CDS             complement(2306..2566)
                     /locus_tag="PK610_12155"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
BASE COUNT          658 a          540 c          584 g          872 t
ORIGIN      
        1 gggaaaactt ttaacagaag gacggatcta aggatggcta aatttgtcaa gtcttgttta
       61 ccaaattcat ttattaaatc gatctgaagc caccccttat ttagctacat ttaaaaactg
      121 aggaaattct tcaacatttt ttacatattt ctgattgcgc aatgccaatc aaagataaca
      181 tccatccttc tctccatctg tgatgcatcc cccatcaatt cccctgtttg atcctgtcca
      241 gctcgtcttt tgctccgctt tcttttcgct ggttctgaat ttttgcatta ccgaatttgt
      301 aactgaagga taatgtaaat accctcgaat cgctccaggc ttccatattg atgtccatat
      361 tattaaagaa caccgttccg cggaatctgc ttttccagaa tagatcgccg gcactgaatt
      421 taatggtgcc tttttctttc atgatttttt tctgtactcc cagactaatg ctcgacattg
      481 gtttcattat gaatacgcca tagacttgtt tcgacatata gaaggcactc aattcagcac
      541 tccaaccctt gctaaaagtg aactggtttg tcgaattgat catccaggac cacatgccta
      601 catcaaaact ttgacctgaa atttttccgt tgtactggtt atggtaggcg gtaatgaaat
      661 tcatgctcat ccaccatttg gaaatcggga tgggtagcat ggaggagatg gtccagtttt
      721 cgagtgaatt cagattttcg ttcgttgcaa atgttgttcg tgtggaatcg acctgtgagg
      781 tgacttgcgt aatcactcct gtagtgtgcg aatagccaac agttgtggta aggaatccca
      841 taaaggtgtg tccaacttcg aatgcattgg taaattgtgg ttgcagcaag gtgtttcctc
      901 gcatgtaagt gtagggatcg aggaaataga ggaaaggatt cagatcgcgg tagctcggac
      961 gatcgatgcg tcgggaatag ctaaagttga tttcgttttt cttgttgatg gtataactca
     1021 ttgccccgct tggaaaaagt tgtgtgtaat tcctggtgaa tacggaatca atagtgatct
     1081 gttcacctcg tgcgcgggtg ttttccactc gtaaaccaaa ttgcaatcct attttatcac
     1141 tcagctggta ctgtccgttt aaatatgcgg cgttgatgtt ttcggtgtat ttaaaatgat
     1201 tcgtttttgt ggtgtcgaca tattctgttc cgttttctac gatccagtaa cgggcgttat
     1261 tgtcggtgct gacgaaactt gattttattc cggtttccag tcgtagtttt ttattgatgg
     1321 attttacata atcggctttt gcgcttttga tgtctacttt ccccggtaat tcactgcgga
     1381 gtatgagcgg tataccaaat ggggtaccgc cggcatcgaa gtaatcggta acaaaaaact
     1441 gtgggttact ctgatcgaat atggcgtaat cgaggttgac ttttagttct cctcctgttg
     1501 tgtcggtaac ccagcggtaa ttgatgttgc tgttgaaatt actccatttt tcactgtttt
     1561 ttccattcgt ttgagaggaa gaaagcggtc ctccgatggc atcggtgata aatgtattgt
     1621 tcgtattgcg atcacctccg gttgtttgca tggcgtttac gaaaaacccg atggtgtggt
     1681 ttttatgcgg ataaaaatcg actccgaatt ttccgctgtg cgactgactc gtgtcttttc
     1741 cttgcgaggt ttgtttgaaa atactttcta cctgttcgtt ctcacgaaat ttgcgcgaga
     1801 gatcgaacca attggcggag tttcttctgc cgtaattgta cgatccgaaa aaattccatt
     1861 tttcagtacg gtggttgaga ttgaaacctg cattttctct gtcgtaaaaa ccctgtgtgt
     1921 aggaaacata aacggaaccg ttgctgccga gcattttgtt tttcttcagt acaatattga
     1981 tcacacctcc ttgtccggaa gcatcgtagc gggcagaagg gttggtaatg atctcgatgc
     2041 gctcaacctg gtcagccggc aaattggata gcatggtaga gacatcctga ctcgataaat
     2101 aggtcggttt accgtcgatc atgatagtga gtcctgattt tcccttgagt gaaatgtttc
     2161 cgtctttatc tatcatcact ccgggtgatc tgcgcaaaac ttcaagggca gtatttcctg
     2221 cagatacgat ggagttttct acgttcacaa tggtcatatc gaacctgcgt tcaataaatg
     2281 gtttttgtgc aacaacagta atttcttcga gtgattccga actttcttta agaacaatct
     2341 ttccaagtga tttcgtttct ccttccgcaa gttccaatgg accaaaatag cttttcgtat
     2401 agccaattga tgaaataagc accaggaaat ttccggcgtt cagatcttcc agttgaaagc
     2461 ttccattttc atcggtcgta gtacctttta taagtgaaga atcttttgct tccagtaaaa
     2521 ggatgttgac gaaagggagc gcggcatctt tactatccgt tacagaccct gaaagtgtag
     2581 cgttctgggc ttattaggtt ggatcattaa caggaagggt taccggactc aagcgcttta
     2641 tttgcgagaa aaac
//
LOCUS       DAOMYG010000433         2651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_14517_length_2651_cov_9.306240, whole genome shotgun sequence.
ACCESSION   DAOMYG010000433 DAOMYG010000000
VERSION     DAOMYG010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14517_length_2651_cov_9.306240"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2651
                     /locus_tag="PK610_12160"
     CDS             <1..>2651
                     /locus_tag="PK610_12160"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRJ36737.1"
                     /translation="VVVLPSKRGGLFLRKHLASQLQKAFIPPLVSTPTELFSQWSKYR
                     VGDPMELMFLLFESYQSVLGNRAEPFDRYLRWGQMLQTDFNDIDRYLVDTRALFRDLR
                     NIKAQEIEIEGWSFEGEDLSRNQERYLEFWNEMPAIYAAYKKRLQERGLAYGGMLYRD
                     VAENVAERVGTESKEYWFAGFNALSTAEQVIINHLCETEKAQVFFDADPFYLDDKDHE
                     AGMFLRRFRTQWKGRGFSFLKEHFKSEKSIEILNVPGNVAQARIAGQILSEEGGGTET
                     ALVLANEQLLLPVLNSLPEVVDKVNVTMGFPLRSSDLFQLYLLIIELQRSRIDANGKD
                     KGGVYYRPFLELLFHPVIRQYLGREASLIRDAVHRERRVFVTDKDIERYGGDEFASIR
                     FLLKPWGDFPSDALRSFVVLNDLLRDFYLHRKKDKLALEYVFHLSSAIDKLKVICEKY
                     PYVTEFHSFRTLLRNYVSQLDISFLGEPLDGLQLMGMLETRGLDFEKVILLSVNEDVL
                     PKSRWEHSFILYELKRYYGLPTYKERDAVYAHHFFRLLQRADKVWLIYNSDPENFSSG
                     EKSRFVLQLQEELPKYYPKISIRERIVQLPMDKHDISVSGIEKTEEIINRIKEHLQSG
                     VSPSALNKFVTCPLDYYYRYVLGIKEDEELDEVSDHAVVGTVVHGVLEQQYKELIGKE
                     VNPETVKQFRKESRRLAREELKKHAGSVVEEGAYYLLLQVAMSFLDNFYEAELDTLET
                     NSIQILSIEEKLSAQLEVDGLAIKISGKADRIDVCNGVLRIIDYKTGKFESKDVSVKV
                     GSEILDKGKALQLLAYAWMRKKMNQDETLPISAGIFPLRNSEEVALLEFGKSTQLNES
                     AFTAFEDAIRQLVGQLLDPAQAFVHDP"
BASE COUNT          744 a          459 c          677 g          771 t
ORIGIN      
        1 cgttgttgtg ttacccagta aacggggagg attgtttttg cgcaaacacc ttgcctcaca
       61 actgcagaaa gcatttattc cgcctttggt tagcactcca accgaattgt tttcccaatg
      121 gagcaagtat cgtgtgggcg atcccatgga gttgatgttc ttgttgtttg aatcctatca
      181 atcggtattg ggtaatcgtg cagaaccgtt cgaccgttac ctgcgttggg gacaaatgct
      241 gcagactgat tttaacgata tcgatcgtta cctcgtagat acccgtgcat tgtttcgtga
      301 tcttagaaac ataaaagcgc aggaaataga aattgaagga tggagttttg agggagaaga
      361 tttatcgcgg aatcaggaaa gatatctgga gttctggaat gaaatgcccg ctatttatgc
      421 agcatacaaa aagcgattgc aggaaagagg tctggcttac ggcggaatgc tatataggga
      481 tgttgctgaa aatgtggcag agcgggtagg gacagaaagt aaagagtatt ggttcgccgg
      541 attcaatgca ctttcaactg ccgagcaggt tatcatcaat catttgtgcg aaacggaaaa
      601 agcgcaggta ttttttgatg cagatccttt ttatctcgat gataaagatc atgaggcagg
      661 aatgttcttg cgacgattca gaacacaatg gaaagggcgt ggattttcat ttttgaaaga
      721 acattttaaa tctgaaaaat caattgaaat tctcaatgtg cctggcaatg ttgctcaggc
      781 gcgtatagca ggacaaattc tcagcgaaga aggtggaggc accgaaacag cattggtgct
      841 tgccaatgaa cagttgttgc ttccggtttt aaattcgctt cctgaggtgg tggataaggt
      901 gaatgtcacc atgggttttc ccttgcggag cagtgatctg tttcagttgt atctgcttat
      961 tatagaattg cagcgttcgc gaattgatgc taatggaaaa gataaaggtg gggtttatta
     1021 ccgtcctttt cttgaactgt tatttcatcc cgtaattcgt caatacctgg gcagggaagc
     1081 aagtcttatt cgcgatgctg ttcaccgtga gcgaagggtg tttgttaccg ataaggatat
     1141 tgagcggtat ggaggagatg aatttgcatc tattcgtttt ttgctgaagc cttggggtga
     1201 tttcccttct gatgcgctgc gttcttttgt tgtgctgaac gatttgctta gggattttta
     1261 cctgcatcgg aaaaaggata aacttgcgct cgaatacgtt tttcatttga gttcagcaat
     1321 tgataagctg aaagtgatct gtgaaaaata cccttacgtt accgagtttc attccttccg
     1381 gacattgttg cggaattatg tgagtcagct cgatatttca tttttgggtg agccattaga
     1441 cggattgcaa ctgatgggaa tgcttgaaac ccgcggactg gattttgaga aggtgattct
     1501 tttgtcggta aatgaagatg tattgcctaa atcgcgatgg gaacattcgt ttattctcta
     1561 tgaactcaaa cggtattacg gattacccac atataaggag cgggatgcag tgtatgcaca
     1621 tcactttttc cgtttattgc aacgggcaga taaagtctgg ttgatttaca attcggatcc
     1681 ggagaatttt tcgagtggtg aaaaaagtcg ttttgttttg caactgcagg aggaattgcc
     1741 gaagtattat ccaaaaatca gtattcgcga acgcattgtg cagcttccaa tggataaaca
     1801 cgatatcagc gtttcaggaa ttgaaaagac ggaagaaatc atcaaccgca tcaaggaaca
     1861 tttacaatca ggtgtttcgc cttccgcgct caataagttt gttacttgtc cgcttgatta
     1921 ttattaccgc tatgtattgg ggataaaaga agatgaagaa ctggatgaag tcagcgatca
     1981 cgctgttgtg ggtacggtag tgcacggtgt tttggagcag caatacaaag aattgatcgg
     2041 caaggaagtt aatccggaaa ctgtaaaaca attcagaaaa gaatcgcgga gacttgccag
     2101 ggaagaattg aagaagcatg ccggttctgt tgttgaagaa ggtgcgtatt atctgttgct
     2161 gcaagtggcg atgagttttc ttgacaattt ttacgaagcc gaactggata cacttgaaac
     2221 aaatagcatt cagattttat cgatcgaaga gaagttgtcg gcacaactgg aggttgatgg
     2281 tcttgcgata aagatcagcg gcaaggccga ccgtattgat gtatgcaatg gggtattgcg
     2341 gataatcgat tacaaaacag gaaagtttga gagcaaagat gtaagtgtaa aagtaggttc
     2401 ggaaattctg gataaaggaa aagcgcttca attgctggcc tatgcctgga tgcgtaaaaa
     2461 aatgaatcag gatgaaacct taccaatcag tgcaggtatt tttccattgc gaaacagtga
     2521 agaagttgca ttgctcgaat ttggaaaatc aacgcagttg aatgaaagtg catttacagc
     2581 ctttgaggat gctattcgtc aattggtagg gcagttgctc gatcccgccc aggcttttgt
     2641 tcacgatccc g
//
LOCUS       DAOMYG010000434         2648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_14542_length_2648_cov_9.025453, whole genome shotgun sequence.
ACCESSION   DAOMYG010000434 DAOMYG010000000
VERSION     DAOMYG010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14542_length_2648_cov_9.025453"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PK610_12165"
     CDS             <1..316
                     /locus_tag="PK610_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36738.1"
                     /translation="FGFFLFNTIMKKILNLTYWLVILGIAILCGLIFFNVVDFGNLYG
                     AFIHFFGGLTVIALLLILALFIKFSKSEKTKLVILIIGNTIGFLVIITITYLFTIGKG
                     PY"
     gene            complement(332..1234)
                     /locus_tag="PK610_12170"
     CDS             complement(332..1234)
                     /locus_tag="PK610_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HRJ36739.1"
                     /translation="MIKVAWRKEYVQALPEGHRFPMEKYALLPQQLLYEGTIGEENIF
                     YPESIDLSVCRLTHKDEYLDKLLHLRLDKNEIRKMGFPLNEALIQRELMIMEGTRKCA
                     EFALQNGIALNTAGGTHHAFSDRGEGFCLLNDNAIAANWLLHSGKAKKILIVDLDVHQ
                     GNGTAELFANEDRVFTLSVHGEKNYPMHKEESDLDIGLPDGIGDVEYLNVLRSTIDPL
                     IEKFKPDFLFYQCGVDILASDKLGRLGISHDACKERDRIVLSASHRRAIPVVCSMGGG
                     YSERIAAIVDAHANTFRVAKELWG"
     gene            complement(1268..2209)
                     /locus_tag="PK610_12175"
     CDS             complement(1268..2209)
                     /locus_tag="PK610_12175"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HRJ36740.1"
                     /translation="MAPKKTILFLFPLILLVLVACERDVDIEIPGGTRQPAVYGWIEP
                     GAPPIIIFTRAQPYFGSSNFSSFEELFIHNASITVSTDNYSALLFELCSNSIPDSLLP
                     VVGQFLGLDSITLASVNYCVYTSFDANIFGVVGKNYYLNIATTEGEQLSAVTKIPQPV
                     ALDSLWFQPERGDTLGFIWARLTDPDTLGNAYRWFAQRQGKDNGFIAPYGSSFDDKFI
                     NGTSFNFGVPRGDNDFAPTPEVEGERGYFKVGDTVIAKFCTIDMAHFQFWRSFDTQLA
                     SNGNPFAAPAPLRSNITGGLGIWGGYSPSYDTLIITP"
     gene            complement(2212..>2648)
                     /locus_tag="PK610_12180"
     CDS             complement(2212..>2648)
                     /locus_tag="PK610_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ36741.1"
                     /translation="DLNSGEWFYAKFDRRNDVSLALVYEPNKKWTFGAVFVYAQGNAI
                     TLPYSWYMVQDQVVFEFAPRNSLRMPAYHRLDLSATRKGKETKKFKSSWNFSIYNVYS
                     RLNPYFLYVDISGSPIDGTLTIKAKQVSLFPILPSVTWNFSF"
BASE COUNT          792 a          591 c          507 g          758 t
ORIGIN      
        1 attcgggttt tttcttttta atacaatcat gaagaaaata ctgaacctaa cttattggct
       61 tgttatcctt ggaatcgcca tattatgtgg acttatattc tttaatgttg ttgatttcgg
      121 caatttgtat ggtgccttta ttcatttttt tggcggactt acagttattg cacttttgtt
      181 gatacttgct ctctttatca aattcagcaa aagcgaaaaa acaaaactgg tcattctgat
      241 aattggaaat acgattggat ttctcgtgat aataactatc acttatcttt ttacaatagg
      301 aaaaggtcct tactgattta tatagtccaa atcaacccca caattccttc gcaacccgaa
      361 aagtgttggc gtgagcatca acgatagcag caattcgttc ggaataacca cctcccatcg
      421 aacaaaccac aggaatcgca cgacggtgtg aggctgatag aacgatacga tcgcgttctt
      481 tgcatgcgtc atgactaatg cccaacctgc ccaatttatc actggccaga atatcgactc
      541 cgcattggta aaaaagaaaa tcaggtttaa atttttcaat caacggatca atggttgatc
      601 gcagaacatt caggtattca acatctccaa tcccatcagg cagaccaata tccaaatcgc
      661 tttcttcctt gtgcatcggg tagttctttt caccgtgaac agagagtgtg aaaaccctgt
      721 cttcgtttgc aaaaagttct gctgttccat ttccctgatg aacatccaga tcgacaatca
      781 gaattttttt tgcttttcct gaatgcagca accagtttgc agcaatggcg ttatcgttca
      841 acaaacagaa tccttctccc ctatcggaaa aagcatgatg tgttccgcct gcggtattga
      901 gtgctatacc attctgtagt gcaaactcag cacatttgcg ggttccctcc attatcatta
      961 attcacgctg aatcaatgct tcatttaaag gaaatcccat tttacggatt tcgtttttat
     1021 ccagtcttag gtgaagaagt ttatccaggt attcatcctt atgggttaac ctgcaaacgg
     1081 aaagatctat tgattcagga taaaaaatat tctcctcacc aatggttcct tcatagagca
     1141 attgctgagg caacaacgca tatttttcca taggaaaacg atgtccttcc ggaagagcct
     1201 gcacgtattc tttgcgccat gctaccttaa tcataccatg cattcattga ttctctgcaa
     1261 tcacggatca gggagtaatg atcagcgtgt cgtaacttgg agaataacca ccccagattc
     1321 cgagtccacc cgttatattg cttctaagcg gagcaggtgc ggcaaaggga tttccattgc
     1381 tggcgagttg tgtatcgaag cttctccaaa attgaaaatg cgccatatca atcgtgcaga
     1441 attttgcaat gacagtatct cctaccttaa aataaccacg ctcaccttct acctctggcg
     1501 ttggagcgaa atcattatca ccacggggaa caccaaaatt aaaactggtt ccgttaatga
     1561 acttatcatc aaaagatgat ccgtagggag caataaaacc attatcctta ccctgacgtt
     1621 gtgcaaacca gcggtaggca tttccgagtg tatcaggatc agtaagccgc gcccagataa
     1681 agcctaaagt atcccctctt tccggttgga accataaact atcaagcgct acgggctgag
     1741 gaattttcgt aactgcactc aattgttcac cttctgtagt ggcaatattg aggtaataat
     1801 tctttcccac tactccgaaa atgttggcgt caaaactggt ataaacacaa taattcacgc
     1861 ttgccaatgt gatcgaatcc aaaccaagaa actgaccaac caccggcaat aatgaatcgg
     1921 gaatactgtt gctgcataac tcaaacaaca gcgcagaata attgtcggtc gaaacagtta
     1981 tgcttgcatt atgaataaat aattcttcga aacttgaaaa gttggacgaa ccaaaataag
     2041 gttgtgcgcg ggtgaaaata atgattggcg gtgctcccgg ttcgatccag ccatagactg
     2101 caggctgacg cgtacctccg ggaatttcaa tatccacatc gcgttcacaa gctaccaaca
     2161 ccagcagaat tagcgggaag agaaacagta ttgttttttt cggagccata atcaaaaact
     2221 aaaattccag gtgacagaag gaagaattgg aaagagcgaa acctgttttg ccttaatggt
     2281 aagtgttccg tcaatcggac ttcctgaaat atctacatag aggaaataag gattcaagcg
     2341 gctgtacaca ttgtaaattg aaaaattcca gctcgattta aatttcttgg tttcttttcc
     2401 tttgcgggtt gcactcagat caagacggtg ataagccggc atccgaagtg aatttcgggg
     2461 agcaaactcg aataccactt gatcctgtac catgtaccag gaataaggaa gcgtaattgc
     2521 atttccctgc gcatagacaa acacagcgcc gaatgtccat tttttatttg gctcataaac
     2581 caatgcaaga cttacatcgt ttcttcgatc aaactttgca taaaaccatt caccgctatt
     2641 gagatcat
//
LOCUS       DAOMYG010000435         2644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_14572_length_2644_cov_8.873696, whole genome shotgun sequence.
ACCESSION   DAOMYG010000435 DAOMYG010000000
VERSION     DAOMYG010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14572_length_2644_cov_8.873696"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PK610_12185"
     CDS             <1..359
                     /locus_tag="PK610_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36742.1"
                     /translation="AQGHIANGLAAIFVATGQDVACVSEASVGLTRIERTSSGDIYGT
                     VTLPNLIVGTVGGGTHLPTQRECLEMVDCLGSGKARKFAEIIAAVVLGGELSISAALS
                     AGHYAKAHKDLARKKA"
     gene            454..1320
                     /locus_tag="PK610_12190"
     CDS             454..1320
                     /locus_tag="PK610_12190"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRJ36743.1"
                     /translation="MRIYLSTFLFVFGFSQLINAQSFNSYQKAPAPGTIQFGPDLFID
                     RTEISNAAWLEYRMWLKSIFGEDSKEYRSAQSDTLVWSKYNRCLADFGKVYSRHPAYR
                     DFPVVGVTQKQAEEFSKWRSDRVMEGYLIKFKIILRQAQDKDNYFTIEKYFNGKYLDL
                     KPDPRIKYYPEYKLPSESDFKQALEHNAKLKGKYKEQPILDVNPCDTQRGVRIKDPVA
                     PVTVIAKGKKKNHIDLLRGNVAEWTSASGVCFGLSWIHLHEDLKNGDLIEVKTVQAWV
                     GFRNVCVWKEWK"
     gene            complement(1337..2206)
                     /locus_tag="PK610_12195"
     CDS             complement(1337..2206)
                     /locus_tag="PK610_12195"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRJ36744.1"
                     /translation="MKYRILIFCLYFSSLSNAQQLPVQTLWWNTQSIYNPAYSGLHNK
                     QAAGISGRYQWTGIEHPYESALAFYDQQLFSEKMGIGGNYSYNHVGNTQTSNRFHVNL
                     NYAFRFKHSSLRIGIAPGIQRLRMDYPAWITTSPQPDPALPQSGAQTKFSFNSGVAWT
                     NDKLSLNFGVCQLTKPYFDKVKFHSAPEYFLGLTYSTALPNGSTLNPSFHMEKNGPSS
                     QIQTALFFHHQKGPQLGGFWRRQDSSGVIIGFQKKNPFLFQYVFEIATGSSAQFRKYS
                     HELHIVYLLKKEI"
     gene            complement(2203..2568)
                     /locus_tag="PK610_12200"
     CDS             complement(2203..2568)
                     /locus_tag="PK610_12200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ36745.1"
                     /translation="MKILIVEDELLIARVYAIHLKSAGHTVLGSVTHPDPARELISKE
                     LPDALVLDINLKGGADGIAFARELRETYSFPILFTTGNSKIRTLEEISDISNCDVMSK
                     PIEATALIKRLKEMTSNLP"
BASE COUNT          732 a          504 c          614 g          794 t
ORIGIN      
        1 gggcgcaggg acatattgct aacggactgg cggcaatttt tgttgctacc ggtcaggatg
       61 tggcttgtgt ttctgaagcg agtgttggat taacgcgtat tgaacgaact tcttctggtg
      121 atatttacgg aaccgttacc ttgccaaatc ttattgttgg aacagttggg ggaggaacac
      181 atttaccaac tcagcgtgaa tgtctcgaaa tggtggattg tctgggttca ggtaaagcac
      241 gtaagttcgc ggaaattatt gctgcagttg ttttgggagg agagctcagt atttcagctg
      301 cactttcggc agggcattac gcaaaggcac acaaagatct tgcgcgtaag aaagcttaag
      361 tggttttcca atgaaaaaat tttattcccg cattaaactg ccgttggttt ggttcgcctt
      421 gttattttca tcctttttat ttaaattcaa cacatgagaa tctatttatc tactttttta
      481 tttgttttcg gtttttccca actgatcaac gctcaaagtt tcaacagtta ccagaaggca
      541 cctgcaccgg gtacaattca gtttggtccg gatcttttta ttgaccgcac tgaaatctcc
      601 aatgcagcat ggttggaata cagaatgtgg ttaaagagca ttttcggaga agactctaag
      661 gaataccgct ctgcacaatc cgatacttta gtttggtcca aatacaatcg ttgtcttgct
      721 gatttcggaa aggtgtattc gcgtcacccc gcttaccgcg attttcctgt tgttggagtt
      781 acacagaaac aagccgagga attttccaaa tggagatcgg atcgcgtaat ggaaggatat
      841 ctgattaaat ttaaaatcat tcttcgtcag gcgcaggaca aagacaacta ttttacgatc
      901 gaaaagtatt tcaacggaaa gtatctggat ttaaaaccag atcctcgtat caagtattat
      961 ccggagtaca aacttccttc cgaaagcgat tttaaacagg cattggaaca taatgcgaaa
     1021 ttgaaaggga aatacaagga acaacctatt ctcgatgtta atccatgcga tacgcagcgt
     1081 ggagtacgta taaaagatcc cgttgcgcct gttacggtta ttgctaaggg aaagaaaaag
     1141 aatcacatcg atttgcttag aggtaatgtt gccgaatgga catcagcatc aggagtttgt
     1201 ttcggtttat cctggattca tttgcatgaa gacctaaaga acggtgatct catcgaggtg
     1261 aaaaccgtac aagcctgggt tggcttccgc aatgtttgtg tttggaaaga gtggaaatag
     1321 aaggcaacaa taatcactat atctctttct tgagtagata aacaatatgt aattcgtgag
     1381 aatattttcg gaattgtgca gatgaacctg ttgctatttc gaatacatat tggaaaagaa
     1441 aggggttttt cttttgaaat ccgataataa caccagaaga atcctgtcgc ctccaaaaac
     1501 cccctagttg tggacctttt tgatggtgga agaaaagtgc agtttggatt tgtgatgatg
     1561 gaccattttt ttccatatga aaggatggat ttaaggtgct cccgttggga agcgctgttg
     1621 agtaagttag tccaagaaag tattcaggtg ccgaatgaaa ttttacctta tcgaaatagg
     1681 gcttggtcag ctggcaaaca ccaaaattga gactcagttt gtcattggtc caggctaccc
     1741 cagaattgaa gctgaatttc gtttgtgctc cactctgagg caaggccggg tcgggttgcg
     1801 gagaagttgt gatccaggcc gggtaatcca ttctgaggcg ctgaattcct ggggcgattc
     1861 caatacgtaa agaagaatgc ttaaaccgga aagcgtagtt gagattgacg tgaaatcggt
     1921 tggatgtttg ggtatttccc acgtgattgt aagagtagtt tcctcctatc cccatttttt
     1981 cgctaaaaag ctgctgatcg taaaacgcca gagcggattc gtagggatgc tctattcctg
     2041 tccattggta ccttcccgaa attccggcgg cttgcttatt atgaaggcct gaataggcgg
     2101 gattgtaaat ggattgagtg ttccaccaaa gtgtttgcac aggtaattgt tgcgcattgc
     2161 taagcgaaga aaagtaaaga caaaaaatca gaatgcgata cttcatggta gatttgaggt
     2221 catttcctta agacgtttga ttaaggctgt tgcttcaatg ggtttcgaca tcacatcaca
     2281 attactgatg tcacttatct cttcaagtgt tcttattttg ctgtttcctg tagtaaaaag
     2341 aattggaaag gaataagttt ccctaagctc acgtgcaaag gcaatgccgt cggctcctcc
     2401 ttttaggttg atgtctaaaa ccaaggcatc cggtaattct ttactgatca gttcccgcgc
     2461 cggatccgga tgtgtaacgc ttcccaaaac agtatgaccg gcacttttga ggtgaatggc
     2521 atacacccgt gcaattaata gttcatcctc aacgattagt attttcattc tgttaatcgt
     2581 gtttaaaaag tactgtaaaa caggtttctt tccccgtgct gaatttcagt tcgcctccaa
     2641 gctg
//
LOCUS       DAOMYG010000436         2638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_14632_length_2638_cov_7.275261, whole genome shotgun sequence.
ACCESSION   DAOMYG010000436 DAOMYG010000000
VERSION     DAOMYG010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14632_length_2638_cov_7.275261"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PK610_12205"
     CDS             complement(<1..201)
                     /locus_tag="PK610_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36746.1"
                     /translation="MHIDEIRQLYKDDSRCKEIARGLTSSKCNYKLSGLIGSSEAFFA
                     FGTFENTGGTHLFIFEEKEHAIY"
     gene            complement(250..1248)
                     /gene="fbp"
                     /locus_tag="PK610_12210"
     CDS             complement(250..1248)
                     /gene="fbp"
                     /locus_tag="PK610_12210"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HRJ36747.1"
                     /translation="MTKIKTLGEFIIEKQSDFPFASGELTRLLSSIRLASKIINREVN
                     RAGLTSDILGSAGTENIQGEAQQKLDVYADNQMIYALKARGEVCAIASEENDDYVIFD
                     NDLSRKGKYIVLFDPLDGSSNIDVNVSIGTIFSIYRRLDENKPVGPEDFLQEGKKQVA
                     AGYVIYGSSTMLVYTTGNGVNGFTLDPGIGTFCLSHPNIRIPEDGKIYSVNEGNFGQM
                     EEGVKNYIRYCQEDDKSTSRPYSGRYIGSLVADFHRNLLKGGIYIYPGTMKSPEGKLR
                     LLYECNPLALIAEQAGGKASTGKERILDIVPTKLHQRVPFFAGSKKMVEKAESFLK"
     gene            1363..1842
                     /locus_tag="PK610_12215"
     CDS             1363..1842
                     /locus_tag="PK610_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ36748.1"
                     /translation="MTTRIRQGTPADLPRALELVKELAEYEKAPLEVENTVEEMLKDG
                     FGAHPVFYLLVAELDGTIEGIAIWYYKYSTWKGKCIFLEDIVVTEKARGKGIGSSLFE
                     AVMLKAKEEKVRRMEWQVLDWNEPAIRFYKKYNANLDSEWLNGKLVFDQLQHFDFNN"
     gene            1857..>2638
                     /locus_tag="PK610_12220"
     CDS             1857..>2638
                     /locus_tag="PK610_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRJ36749.1"
                     /translation="MKVFKFGGASVRDAAGIRNVATILRRFSSENLAVVVSAMGKTTN
                     ALEDLTRAYAKGKGDKDAILAKIHEDHIKEMDDLFENNRNHPVYAAVAEVFSGIQKRF
                     DVTASANYDLVYDQIVSQGEFISSVIVSHYMNAQGIENTWYDARTIIRTDSTFREGKV
                     DWDASQKLANQHLIPFYIGSVGRKIAITQGFLGSTSIGLTTTLGREGSDYTAAILAYL
                     LDAKDVTIWKDVAGVMNADPRKYDKAVKFDRISFREAIELSY"
BASE COUNT          751 a          506 c          606 g          775 t
ORIGIN      
        1 gtaaatggca tgttcctttt cttcaaaaat aaaaagatgg gttcctccgg tattttcaaa
       61 agttccaaat gcaaagaatg cttctgagga accaataagt cctgataatt tgtaattgca
      121 tttagatgaa gttaaaccgc gtgcaatttc tttgcagcgt gaatcatctt tgtaaagttg
      181 tcgtatttcg tcaatatgca tagcgctcct tccggcagtg ctttttgtat aaggaatttg
      241 cagcgttctt tacttcaaaa aggattctgc tttttcaacc atcttttttg atccggcaaa
      301 aaacggtact ctttgatgga gttttgtggg gacgatatcc agaatccgtt cttttcctgt
      361 cgatgctttt cctccggctt gttctgcgat cagagcaagt ggattgcatt cgtacaacaa
      421 gcgcaattta ccttcgggtg atttcattgt tcccgggtag atgtaaatcc cgccctttag
      481 caggttgcgg tgaaaatcgg cgactaatga gcctatatag cgaccggaat atggacgtga
      541 agtggatttg tcgtcttcct gacaatagcg aatataattt tttactcctt cttccatttg
      601 tccgaaattc ccttcgttaa ccgaatagat ttttccatct tccggaatac gaatgttggg
      661 atgcgataaa caaaacgttc cgataccagg atcaagcgta aaaccgttta ctccgtttcc
      721 ggtagtatat accagcatgg ttgaagaacc gtaaatgaca tatcctgctg caacttgttt
      781 ttttccttcc tgtaaaaaat cttcaggacc tacaggtttg ttttcatcga gtcggcggta
      841 aatggaaaaa attgttccaa tggaaacatt tacatcgatg ttcgagctgc cgtcgagtgg
      901 atcaaacaat acgatgtatt tgccctttcg cgacaggtca ttgtcgaaga ttacataatc
      961 atcattttct tcagaagcaa tggcacaaac ttctccgcgt gctttcaggg catagatcat
     1021 ctggttgtcg gcgtaaacat ccagtttttg ctgggcttca ccctgaatat tttctgtacc
     1081 ggctgaacct agaatatcac tggtcaatcc tgcacggttc acttcccgat tgatgatttt
     1141 ggaggccagg cgaatagaac tgagtaatcg ggttaattca ccactggcaa aagggaaatc
     1201 ggactgtttc tcaatgataa attcacctaa ggtcttgatc ttggtcatgc tgctttaaat
     1261 agaatgcgaa tatcggagat tttgggcata aagtcaggtc aaatagggca agcataacga
     1321 tatttttacg cgagaagggc tacatctttc tatttttgca ccatgacaac acgaattcga
     1381 cagggcacac ccgctgatct cccaagggca ttggagcttg ttaaggaatt ggccgaatac
     1441 gagaaagctc ctttggaagt ggaaaatacg gttgaggaaa tgctcaaaga tggttttggt
     1501 gctcatcctg tattttatct gctcgttgcg gagcttgacg gaactattga ggggattgcc
     1561 atttggtatt acaagtattc tacctggaaa ggaaagtgta tttttctgga agatattgtt
     1621 gtaacggaaa aggcaagagg aaagggaata ggatcttctt tgtttgaagc tgtaatgctt
     1681 aaggcaaaag aagaaaaagt gagaaggatg gaatggcaag tgctcgattg gaatgaaccc
     1741 gctattcgtt tttacaaaaa atacaacgca aacctcgatt ccgaatggct gaacggaaaa
     1801 ttggttttcg accagctgca gcattttgat ttcaacaact aatacaataa agattcatga
     1861 aagttttcaa gtttggtggc gcaagtgtaa gggatgcagc aggaatacgg aatgttgcaa
     1921 ccattttaag gcggttctcc agtgagaatt tagctgttgt ggtttctgct atggggaaaa
     1981 ccactaatgc ccttgaagat ttgacacgtg cctacgctaa gggaaaggga gataaagacg
     2041 ccatacttgc aaagatccat gaggatcata tcaaggagat ggacgacctc tttgagaaca
     2101 atcgaaatca tcccgtttac gctgcagttg cagaggtctt ctccggaata caaaaacgat
     2161 ttgacgtaac ggcatcggca aactatgatc tggtttatga tcagattgta tctcagggtg
     2221 agtttatttc ttctgttatc gtcagtcatt acatgaatgc tcagggaata gaaaatacct
     2281 ggtacgatgc acgcaccatt atccgcaccg atagcacctt tcgggaagga aaagtcgatt
     2341 gggatgctag ccagaaactg gccaatcaac atcttattcc cttttatatc ggatctgttg
     2401 gacgaaaaat tgcaattaca cagggatttc tcggtagtac cagtattgga ttaactacaa
     2461 cactcggacg tgaaggttcg gattatacag ctgccatact tgcatactta ctcgatgcga
     2521 aggatgtaac gatttggaaa gatgtggcag gggtgatgaa tgcagatccg aggaaatacg
     2581 ataaggcagt gaaattcgac aggatctctt ttcgggaggc tatagagttg agttatta
//
LOCUS       DAOMYG010000437         2633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_14674_length_2633_cov_7.759116, whole genome shotgun sequence.
ACCESSION   DAOMYG010000437 DAOMYG010000000
VERSION     DAOMYG010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14674_length_2633_cov_7.759116"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="PK610_12225"
     CDS             <1..1105
                     /locus_tag="PK610_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRJ36750.1"
                     /translation="LYKGKTLIVGGSAQVNYLNDDSYFGDLSAIIVKNLPFWVEFRRT
                     PSREIALMTNWEEKIEKMARGTMDEDVYILAGVPSWTLVLLRRILDIKGKDNIAEIWP
                     RLELFMHGGVSFAPYKEQFKKIIRKDEMNYVETYNASEGFFGIQDTTDKEEMLLMLDY
                     GIFYEFIPMEEYGKSNPKTVDLSGVIVGKNYAMVISTNAGLWRYCIGDTVRFTSTSPY
                     RFVITGRTKQFINAFGEELIVDNAEKAIAIASERTNAMVKEYSVAPVFMSSVEPGTHE
                     WVIEFEKEPAELTFFAEVLDNALKALNSDYEAKRSNNLNLRFPIVHNAPNGTFYAWLK
                     ERGKLGGQHKVPRLANNREYIESIISIMNKLRK"
     gene            1102..1890
                     /locus_tag="PK610_12230"
     CDS             1102..1890
                     /locus_tag="PK610_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36751.1"
                     /translation="MILLFFFSLFAQPDSSALQWKAEKEFQLKSTFITADEMGNLYVV
                     NKDEITKYGSKGDSICTQSFKTMGWISLLDVTNPLRPMIFYRDLNQVVVLDNTLSVQG
                     EPVKLEKLGLQFTKLFCQSPNNQNLWLYDTDEFRLMRMDKNFQLVRSSGNITQVTGRD
                     LNPTHMQEWNNFLYLSDPDQGILVFDMFATYSKTIPLKNIRNFQVTNGGIFYLEDNSL
                     LRYDLMSFESMKIPLPLNTVESFRISGKRLYLQGSSKISVFSLQ"
     gene            1980..2630
                     /locus_tag="PK610_12235"
     CDS             1980..2630
                     /locus_tag="PK610_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRJ36752.1"
                     /translation="MHIAIAGNIGSGKTTLTTLLANHYKWEAHYEDVSNNPYINDFYE
                     DMQRWSFNLQIYFLNTRFQQIQDIRQNNVSIIQDRTIYEDAYIFAPNLHAMGLMTSRD
                     FENYYSLFSHMERFIQAPDLLIYLRSSVPTLVNHISARGREYEESIRLDYLKRLNERY
                     EAWISQYDKGKLLVIDVDNNKFHSSKEDLGQIIRSIDAEVGSGLFEIPEKKKGVKK"
BASE COUNT          829 a          528 c          550 g          726 t
ORIGIN      
        1 actgtacaag ggaaaaacat tgatcgtagg cgggtctgca caggtcaatt acctaaacga
       61 tgattcttat tttggagatc tttctgccat cattgtaaaa aatcttcctt tttgggttga
      121 attcagaaga acacccagca gggaaattgc actgatgacc aattgggaag agaaaattga
      181 aaagatggca agaggaacaa tggatgagga tgtgtatatt cttgcaggag ttccatcctg
      241 gacactcgtc ttacttagaa gaattctcga tataaaaggg aaagataata ttgcagaaat
      301 ctggccacgt ttagaattgt ttatgcacgg cggtgtgagt tttgctcctt acaaggaaca
      361 attcaaaaaa atcatccgaa aagatgaaat gaactatgta gaaacctaca acgcatcaga
      421 aggatttttc gggatacagg atacaacaga taaggaagaa atgctattga tgcttgatta
      481 tggcattttt tacgagttca ttcccatgga ggagtatgga aaaagcaatc cgaaaactgt
      541 agatttgagc ggtgtgattg tgggaaaaaa ttatgctatg gtcataagca ccaacgccgg
      601 actatggcgg tattgcatcg gcgatacagt tcgcttcact tctacttcac cctatagatt
      661 tgttattacg ggcagaacca aacaattcat caatgctttt ggtgaagaac tgattgtaga
      721 caatgcagaa aaggcaattg caattgcttc tgaaaggacg aatgccatgg taaaagaata
      781 ttcagtggcg ccggtattta tgagcagcgt agaaccggga acgcatgaat gggtcatcga
      841 atttgaaaag gaaccggcag aattgacttt tttcgcggag gtgttggaca atgcgctgaa
      901 agcactcaac tccgattacg aggcaaaacg ctccaataac ctaaatcttc gttttccaat
      961 tgtacataat gccccaaatg gcacgtttta tgcttggtta aaagagcgcg gcaaactggg
     1021 aggtcagcat aaagttcccc gccttgccaa taaccgtgag tacatcgaat ccatcatttc
     1081 tatcatgaac aaacttcgca aatgatcctt ctcttctttt tcagtttgtt cgcccaaccg
     1141 gattcctctg cattacagtg gaaagcagag aaggaattcc aattaaagag cacattcatt
     1201 actgcagatg aaatggggaa tctttatgtt gtaaataagg acgaaatcac aaaatacggt
     1261 tccaaaggag attccatttg tacccaaagt ttcaaaacaa tgggatggat cagcctgctg
     1321 gatgtcacca atcccttgcg tccaatgatc ttttacagag atttgaatca ggtagtcgtt
     1381 ttggataata ccctaagtgt tcagggagaa cctgtaaaac tggaaaaact gggattacag
     1441 ttcaccaaat tgttttgtca gagtccgaac aaccaaaatc tttggttata cgataccgat
     1501 gaattcagat tgatgcgaat ggataaaaat ttccaattgg ttcgcagctc cggaaatatt
     1561 acacaagtaa caggccgcga cctcaatccc acacacatgc aggaatggaa taatttcctt
     1621 tacctgagcg atcctgatca aggcattttg gttttcgata tgtttgccac ctattccaaa
     1681 acaatccccc tgaaaaacat ccgaaatttc caggtcacca acggcggaat cttttacctg
     1741 gaagacaatt ctctgctccg atacgacctg atgagctttg aatcaatgaa aattcctctt
     1801 cccctcaaca ccgtggaatc gttcagaatt agcggaaaac gcctttattt acagggcagt
     1861 tcgaagattt cagtgttttc tcttcagtaa ataatcaaga agtttttaac catcttgctt
     1921 taataaccct ttgtcgggtt gaatttttac tattttcgga ttcccaaaac aggtgagcta
     1981 tgcatattgc gattgccgga aacatcggtt caggtaagac tacattgacc actttgctgg
     2041 ccaaccatta caaatgggag gcgcattatg aagatgtatc gaacaaccca tacatcaatg
     2101 atttctatga agatatgcaa cgttggtcat tcaacttgca gatctacttt ttaaatacac
     2161 gattccagca gatacaggat atccgtcaga acaacgtgag tatcattcag gatcgtacca
     2221 tttacgagga tgcctacatc tttgctccca acctgcatgc gatgggatta atgacttcgc
     2281 gtgattttga aaattattat tcgctcttct cgcacatgga gcgtttcatt caggctccgg
     2341 atttactcat ctaccttcgc tcaagtgtac caacacttgt taatcacatc tcggcacgcg
     2401 gacgggaata cgaagaaagt attcgcctgg attaccttaa gcgccttaat gaacgttacg
     2461 aagcatggat tagtcaatac gacaaaggca agttattggt gattgatgtt gacaacaata
     2521 aattccattc ttcaaaagaa gatctcggtc agatcatacg cagcattgat gctgaagtag
     2581 gatccggatt atttgaaatt cctgaaaaga aaaagggagt taaaaagtaa acg
//
LOCUS       DAOMYG010000438         2621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_14759_length_2621_cov_8.482463, whole genome shotgun sequence.
ACCESSION   DAOMYG010000438 DAOMYG010000000
VERSION     DAOMYG010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14759_length_2621_cov_8.482463"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..480
                     /locus_tag="PK610_12240"
     CDS             154..480
                     /locus_tag="PK610_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36753.1"
                     /translation="MLLAESKHVFRYRNVQLCDEFEKYQNYAGVFVLEFNYNSKGDVR
                     LCIDDQESIYARLTEWKEGVTKDGQSFLAAEFLYREKGRVFIFSFNEFESVKISLNKG
                     AYMLFF"
     gene            complement(491..2047)
                     /locus_tag="PK610_12245"
     CDS             complement(491..2047)
                     /locus_tag="PK610_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HRJ36754.1"
                     /translation="MSPRNSGTGEKILRLILGDQLNHDHPWYSDGEDAEYVMMEIRQE
                     TDYVKHHIQKIVGIFLAMGVFADDLRKNGNTVHYISIDDPKNKQNISENILAVLQRGN
                     FSAFEYQLPDEYRLDEYLKSLCKDINIPSRAVDTFHFLTERSELAQFFAGKKQLLMES
                     FYRYMRKKHRVLMNAGEPEGGKWNYDHDNRQSIPKGTLIPPPALFPKNVKRVLSRIKN
                     SGIETMGRIAESDFIWPVTRQENLQLLEWFCDFALPVFGRFQDAILRDQWSLFHSRLS
                     FGMNIKLISPQEVIDAVISAWKKNPKEISLQQVEGFIRQILGWREYMRGIYWKEMPGF
                     ARLNFFNHEHQLPHWYWTGETKMECLKQSVKQSLDFAYAHHIQRLMVTGNFALLAGIH
                     PDEVDQWYLGIYIDAFEWVEITNTRGMSQYADGGIVGSKPYVSSANYIDKMSNYCSSC
                     HYNKKEKLGEKACPFNALYWHFYARNRQLLEKNPRIGMMYWVWDKMKKEERTAILEKG
                     DALLNQLNKI"
     gene            complement(2059..>2621)
                     /gene="folE"
                     /locus_tag="PK610_12250"
     CDS             complement(2059..>2621)
                     /gene="folE"
                     /locus_tag="PK610_12250"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013868985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRJ36755.1"
                     /translation="EEKITAITHHFRAIMETLGLDLRDDSLKGTPHRVAKMYVQEVFS
                     GLNPANKPKIALFENKYRYREMLVEKDITLHSFCEHHFVPIIGKAHVAYKSNGNVIGL
                     SKINRVVQYFAKRPQVQERLTIQIAEELKRVLQTEDVAVIIDARHMCVSIRGIEDTNS
                     STVTGSYHGAFLDESVRKEFLHHISH"
BASE COUNT          697 a          521 c          588 g          815 t
ORIGIN      
        1 tatgataaga ctttttagaa cggaccccaa aattcttaca ataggaatta gtgataaatg
       61 gtttagatct ttcattggtt ttttcaacca ttttaagggg gggtagatga gatgattttt
      121 cattgttgaa aaacattcaa tacttgattt tctatgctac ttgcagagag taaacatgtt
      181 tttagataca gaaatgttca actatgtgat gaatttgaaa aatatcagaa ttatgctgga
      241 gtttttgtgc tggagtttaa ctacaacagt aagggtgatg ttaggttatg tattgatgat
      301 caggaatcaa tttatgcccg tttaaccgaa tggaaagaag gggtaacaaa ggatggtcaa
      361 tcgtttttag cggctgaatt tttgtataga gaaaagggaa gggtttttat ttttagtttt
      421 aacgagtttg agtcggtaaa aatttccctg aataaggggg cgtatatgct gtttttctaa
      481 tcagattgat tcagattttg tttagttgat tcaggagcgc atctcccttc tcaagaatgg
      541 cggtgcgctc ttcttttttc attttgtccc atacccagta catcattccg atccgcggat
      601 ttttttcaag gagctgtcgg ttacgtgcat aaaagtgcca atagagggcg ttaaacggac
      661 aagccttctc tcccagtttt tcttttttgt tgtaatggca ggaggaacag taattgctca
      721 tcttatcgat gtagttcgcg ctgcttacat aaggtttgct tcccacaatt ccaccatcgg
      781 catactgact cattcctctg gtattggtga tttccaccca ttcaaaagca tcaatgtaaa
      841 taccgaggta ccattgatcc acttcgtcgg gatgaattcc ggccagcaag gcaaaattac
      901 cggttaccat gagtcgctga atgtgatgcg cataagcgaa atcgagcgat tgcttaacgg
      961 attgtttcag gcattccatt ttcgtttctc cggtccagta ccaatggggt agttgatgtt
     1021 catgattgaa aaagttgagt cgggcaaaac ccggcatctc tttccagtag attccccgca
     1081 tgtattccct ccaccctaaa atctgccgga taaaaccttc cacctgttgc agggagattt
     1141 ctttcggatt ctttttccat gcagaaataa cggcatcaat gacttcctga ggagagatca
     1201 gtttaatgtt cataccaaac gaaagtcggg aatgaaacaa ggaccactga tcgcgcaata
     1261 tcgcatcctg gaaacgaccg aaaacaggca atgcaaaatc gcagaaccat tcgagtagtt
     1321 gtaaattctc ctgtcgcgta acaggccaaa taaaatcgct ttcggctatt cttcccatgg
     1381 tttcaatgcc ggagtttttt attcggctaa gaacacgctt cacatttttt ggaaataaag
     1441 cgggaggagg aatgagcgtt ccttttggaa tcgattggcg gttgtcgtga tcataattcc
     1501 attttcctcc ttcgggttca ccggcgttca tcagcacacg atgtttcttt cgcatatagc
     1561 ggtaaaagct ttccatcagg agctgttttt ttcctgcaaa gaattgggcg agttctgaac
     1621 gttctgtcag aaagtggaag gtatcaactg cgcggctcgg aatgtttatg tccttacaca
     1681 aggatttcag gtattcatca agacggtatt catccggaag ttgatactcg aaggctgaaa
     1741 aatttcctct ttgaagaaca gcgagaatat tttcggaaat gttttgtttg tttttcggat
     1801 catcaataga gatgtagtga acggtatttc cgtttttgcg aagatcatcc gcaaagaccc
     1861 ccatagccaa aaaaataccg acaattttct gaatgtggtg ttttacataa tccgtttcct
     1921 gacggatctc catcatgacg tattctgcat cctcgccatc gctgtaccag ggatgatcgt
     1981 gattcaattg atcaccaaga ataagacgaa gaattttttc cccggttccc gaattacgcg
     2041 gcgacatcgc ggatgagctc aatgggaaat gtggtgcagg aattccttgc ggacagattc
     2101 atccagaaag gcgccgtggt aagaacccgt tacggtgctg ctgttggtgt cttctattcc
     2161 gcgaatggaa acacacatgt gtcgcgcatc gatgatcacg gcaacgtctt cggtttgtaa
     2221 aacacgtttt agttcttccg caatctgaat ggtgagtcgc tcctgtactt gcggacgttt
     2281 tgcaaagtat tgaacgactc tattgatctt tgataaacca attacatttc cattggattt
     2341 gtaagcaacg tgtgctttac caataattgg aacaaaatga tgctcgcaga atgaatgcaa
     2401 agtgatgtct ttttccacca gcatttcccg gtagcggtat ttgttttcga agagcgcaat
     2461 tttgggtttg ttggcaggat ttaatccact gaagacttct tgcacataca tttttgccac
     2521 gcgatgagga gttcctttca ggctatcgtc gcggagatcg aggccaagtg tttccataat
     2581 ggcccggaaa tggtgagtga tggcagtgat cttttcttca t
//
LOCUS       DAOMYG010000439         2617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_14795_length_2617_cov_7.836846, whole genome shotgun sequence.
ACCESSION   DAOMYG010000439 DAOMYG010000000
VERSION     DAOMYG010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14795_length_2617_cov_7.836846"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PK610_12255"
     CDS             <1..270
                     /locus_tag="PK610_12255"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRJ36756.1"
                     /translation="TDFFPEKPQGKLVFFNGNAKITRMILQKGSTIPEHTSPVNTYLL
                     VLRGKIQFHINDMDIQINEKEAIEFPGSTTHSVFAFEDSVFLLFR"
     gene            267..650
                     /locus_tag="PK610_12260"
     CDS             267..650
                     /locus_tag="PK610_12260"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HRJ36757.1"
                     /translation="MSVEKKDIESRADIEQMVKSFYAKVLADPLIAPFFTNVVNVDWD
                     HHLPVMFIFWESILFDKPVYSGNPMMAHISLHQKSPMKPEHFQRWLLHFETNLREQFQ
                     GPVTERASQRAMSIAAVIQSKTTSI"
     gene            695..2056
                     /gene="hemN"
                     /locus_tag="PK610_12265"
     CDS             695..2056
                     /gene="hemN"
                     /locus_tag="PK610_12265"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HRJ36758.1"
                     /translation="MQNNLIRKYNIPAPRYTSYPTVPYWEKEAPDSEQWIGSISDSWY
                     MVNKGKGISLYIHLPYCESLCTYCGCNTRITVNHKVEEPYIRAVLEEWAMYESAFPET
                     AVIDEIHLGGGTPTFFSPENLRQLILGIQGSAIISNNAHLSFEAHPANTTKEHLEVLY
                     ELGFRRLSLGIQDFDPHVQEVINRKQSEEQVMEVMDQARKIGYTSINFDLIYGLPFQT
                     SHSIQNTVEKVIAMQPDRIAFYAYAHVPWMKPGQRKYSEADLPREEEKLALYETGKKM
                     LLNAGYLEVGMDHFALADDELIHADKRGELHRNFMGYTTTQSKILIGLGASAISDSWF
                     GFAQNHKVVEDYIKAIEERRLPINKGHILTPADLVIRQHILNIMCRRSTSWEKEELQC
                     EELYEGLNRMDEVIKDGLVKVDPFHLKVPDKGKPFLRNICMALDARLHEKQPESRIFS
                     SAI"
     gene            complement(2115..2501)
                     /locus_tag="PK610_12270"
     CDS             complement(2115..2501)
                     /locus_tag="PK610_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36759.1"
                     /translation="MDKIKTEKAYDLHVEHSQWLENLKFYEDEMMIFQNRVSEIAGKY
                     TDTEVLKQVEHFQNQLIVQKNELDELRHSIKDHETYIENKVAHNPAADHKYVNDHSKE
                     RERMETFERLFKEMKLELNKFLSKQM"
BASE COUNT          825 a          551 c          501 g          740 t
ORIGIN      
        1 acagatttct ttccggaaaa acctcagggg aaattagttt tcttcaacgg aaacgcaaaa
       61 ataacgcgga tgatcctgca aaaaggaagc acaattcccg agcacacttc accggtaaac
      121 acgtatttac tggtgcttag aggaaagatt caattccaca tcaacgatat ggatattcag
      181 atcaacgaaa aagaagccat tgaatttccg ggcagcacca cacattccgt ttttgcattc
      241 gaagattccg tatttttgct tttcagatga gtgtcgaaaa aaaagatatc gaatcacgcg
      301 ccgacattga gcagatggtc aaaagttttt acgcaaaagt acttgcggat cctcttattg
      361 caccgttctt taccaacgtt gtaaatgtcg actgggacca tcatcttccg gtgatgttca
      421 ttttctggga aagcatcttg tttgacaaac ctgtgtactc cggaaatcca atgatggcgc
      481 acatatccct gcatcagaaa tcaccaatga aaccggaaca tttccaacgg tggctgcttc
      541 atttcgaaac aaacttacga gagcaattcc aaggcccggt tacagaacgt gcttctcaga
      601 gagcgatgtc aattgctgcc gtaattcagt caaaaacaac ttcaatctga cggaagtcat
      661 acctttttga ttacatcctg ctgaagtttg tttcatgcag aacaacctca ttcgaaaata
      721 caatataccg gctccccgat ataccagtta tcctacagtt ccttattggg aaaaagaggc
      781 tcctgattcg gaacaatgga ttggctctat ttctgattcc tggtacatgg taaacaaggg
      841 caaaggaatt agcttgtaca tccatttacc ttattgtgaa agcttgtgta cctattgtgg
      901 ttgcaacaca cgaattacag ttaaccacaa agttgaagaa ccgtatatcc gagcggtact
      961 tgaagagtgg gccatgtacg aatctgcatt tccagaaact gctgtcattg atgaaattca
     1021 cctgggaggc ggaacaccaa ccttcttcag tcctgaaaat cttcgccaac ttattctcgg
     1081 tatccaaggc tcagctatca tcagcaataa tgctcatctg agtttcgaag cgcatcctgc
     1141 gaacactacc aaagagcatt tagaagtttt atacgaactg ggctttagga ggctttcatt
     1201 gggcattcag gatttcgatc cgcatgttca ggaagtcatt aatcgcaaac aaagcgaaga
     1261 acaggtaatg gaagttatgg atcaagcacg gaagattggt tatacctcca tcaattttga
     1321 tctcatttac ggattaccat ttcagacgag ccattcaata caaaacaccg tagaaaaagt
     1381 aatcgcaatg caaccggatc gtattgcatt ttatgcctat gctcacgttc catggatgaa
     1441 acccggtcag cgtaaatatt ccgaagccga tttgcccagg gaagaagaga aacttgcact
     1501 ctacgaaaca ggaaaaaaaa tgctccttaa cgccggatac ttagaagttg gaatggatca
     1561 ttttgcgctt gccgatgatg aattgataca tgcagataaa agaggcgaac ttcacagaaa
     1621 tttcatgggt tatacaacta ctcaaagtaa aattctaatc ggacttggcg cttcagcaat
     1681 cagtgattcc tggtttggtt ttgcgcaaaa ccacaaagta gtggaagatt atataaaagc
     1741 gatcgaagaa cgtcgactac caataaataa aggacatatt ctcactccag cagatcttgt
     1801 aatccgccaa catatcctca acattatgtg tcgcagatca acctcatggg aaaaggaaga
     1861 acttcaatgc gaagaattgt atgagggttt gaacaggatg gatgaagtaa tcaaagatgg
     1921 acttgtgaaa gtggatcctt ttcatcttaa agtcccagat aaaggaaaac cctttcttcg
     1981 caatatctgt atggctttgg atgcccggtt gcatgaaaaa caacctgaaa gcaggatatt
     2041 cagttcagcg atttaacttt ataagcaaaa aaaaatcccg cgttgcggga ttttactatt
     2101 tgcgattaaa agaattacat ctgcttagaa agaaatttgt tcaactcaag cttcatttct
     2161 ttgaataaac gttcaaacgt ttccattctc tcacgttcct tagaatgatc gtttacatat
     2221 ttatgatcag cagcaggatt atgagctact ttattctcga tatacgtttc gtgatcctta
     2281 attgaatggc gcaactcatc aagttcattt ttttgcacaa tcaattgatt ttgaaaatgc
     2341 tcaacttgtt tcagcacttc cgtatcagta tattttccgg caatctcgga aaccctgttt
     2401 tgaaaaatca tcatctcatc ttcataaaat ttgagatttt cgagccattg cgaatgctcg
     2461 acgtgtaaat catacgcttt ttctgtcttt attttgtcca tgataggtgt tttttcaatt
     2521 aaataccgat ttagatgctg ataaacaata cataccactt atcaacgggt acaaagtaaa
     2581 tcgaaacata agaacaccgg aatgaccttc caccatc
//
LOCUS       DAOMYG010000440         2596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_14962_length_2596_cov_8.155057, whole genome shotgun sequence.
ACCESSION   DAOMYG010000440 DAOMYG010000000
VERSION     DAOMYG010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14962_length_2596_cov_8.155057"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..835)
                     /locus_tag="PK610_12275"
     CDS             complement(80..835)
                     /locus_tag="PK610_12275"
                     /inference="COORDINATES: protein motif:HMM:NF025846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRJ36760.1"
                     /translation="MAESGEKFQRQIGHVLHEKKEDYLTFLFERLSAAGNYFLPLLRE
                     ISTEIIIHLAVVKQAKRSKTYFLDLFEIESEIFRSLKHIEKSIALVNAINNNRELTKE
                     EIVPGEEDKKRERMIRELQSNTSDFDEDEVFVPVRKKKTKKSTTPKEAKPLTRETSFE
                     LFKSGKTISEIASIRNLAETTIASHLATYIKTGEIKIKSLMDPEKIEAIANCIAELET
                     TSLTPVREKLGESFSYQEIRFVMNWMERNEGVE"
     gene            complement(866..2359)
                     /locus_tag="PK610_12280"
     CDS             complement(866..2359)
                     /locus_tag="PK610_12280"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ36761.1"
                     /translation="MQTNDPASLAARFIQNSSRHLFLTGKAGTGKTTFLKRIIEQTHK
                     KAVIAAPTGIAAINAGGVTLHSLFQLPFGAFSPEKIGFEAAGDRFRINTPDTLLRNMR
                     MAERKRKLLREIELLIIDEVSMLRADLLDAIDLLLKNIRRNQQSFGGVQVLFIGDLLQ
                     LPPVIKNEEWTILRQFYTSMFFFDAQVLRNDPPVYIELEKIYRQDDEIFINLLNHLRN
                     DQLTDEDIQLLNSYYKSEFRPHPSELFITLTTHNQKADNLNKSYLESLEGESFFYSAE
                     VKEEFPENSYPIDSRIELKKGAQIMFIKNDPTGQQRFFNGMIATVDQLSEGHVYVKTD
                     NERLKVDPYEWENVKFEVNEGTGEVTEKVIGTFTQLPIRLAWAITVHKSQGLTFTKAI
                     IDPQDAFAPGQVYVAMSRLRSLDGLVLTSPVRQNGIRQDHNVKQFAEKKKAHDELNQL
                     ISSETANYLRHYLNQSFELKPLLKSIEKLPVTEAEEESRSVRLRDKD"
BASE COUNT          683 a          500 c          550 g          863 t
ORIGIN      
        1 cgaccgacct cggccagacc tggaaggagg cgacgaagcc gccggccttc ggtcttgagt
       61 aaaatcttat ttttttgaat tattccactc cttcatttcg ttccatccaa ttcattacaa
      121 agcgtatttc ctgatacgaa aatgattcgc cgagtttttc ccgaactgga gtaagtgagg
      181 tggtttcaag ttctgcaata caatttgcaa tagcctcaat tttctcggga tccatcagtg
      241 atttgatttt tatctctccg gttttgatat aggtggcaag gtgactggca attgttgttt
      301 ctgcaagatt tctgatcgaa gcaatttctg aaattgtttt tccggattta aagagctcaa
      361 aactcgtttc tctggtgagt ggttttgctt ctttcggcgt agtagatttt ttggttttct
      421 tttttctgac aggaacaaac acttcgtctt catcaaagtc tgaggtgttc gattgaagtt
      481 ccctgatcat ccgttcacgc tttttatctt cttcgccggg cacgatttct tctttcgtta
      541 attcacgatt gttatttatc gcatttacaa gtgcaatgga tttttcaatg tgtttgagcg
      601 aacggaagat ttctgactcg atctcaaaga ggtcaaggaa ataagttttt gatcgcttag
      661 cttgttttac aacggcaaga tgaataatga tctcagttga aatttctctg agcagaggca
      721 aaaagtaatt gcctgcagcc gataatcttt caaaaagaaa agtcaggtaa tcttcttttt
      781 tttcgtggag tacatgtcca atctgccttt gaaatttttc tccagattca gccaacggtt
      841 gaaatcttga aaataattcc ttcaatcagt ctttatctct taaccgaact gagcgggatt
      901 cctcttcagc ttctgtgaca ggtagttttt cgattgattt taacagtggt tttaattcga
      961 aggactggtt cagatagtga cgcagataat tagccgtttc agatgaaata agttgattca
     1021 attcatcatg tgcttttttc ttttctgcaa actgttttac attatgatcc tgtcggatac
     1081 cgttttgccg aacaggagag gttaagacca atccatcgag tgagcgtaat cttgacatgg
     1141 caacataaac ctgtccgggt gcaaatgcgt cttgcggatc tattatcgct ttcgtgaagg
     1201 tgagtccctg acttttatgt actgtaattg cccatgccaa ccggatgggt aattgggtga
     1261 acgttccaat tactttttct gttacttctc cggtcccttc attcacttcg aacttaacgt
     1321 tttcccattc gtagggatca acttttaatc gttcgttatc cgtttttacg tatacgtgtc
     1381 cttcgcttag ttgatcaacg gtggcaatca ttccgttgaa gaagcgttgc tgaccggttg
     1441 ggtcattttt gatgaacata atctgcgcac cttttttcag ctcaattcgg gaatcgatgg
     1501 gataagaatt ttcgggaaat tcttctttca cttccgcaga gtaaaaaaac gattctcctt
     1561 ccagggattc cagatagctt ttgttcagat tatcggcttt ctgattatgt gtggtaagtg
     1621 tgataaaaag ttcgcttggg tgcggacgaa attccgattt gtaatacgaa ttcagcaatt
     1681 gaatgtcttc gtctgtaagt tgatcattcc ggaggtgatt caataagttg atgaatattt
     1741 catcatcctg cctgtatatt ttttcgagtt caatataaac cggtggatca ttccggagta
     1801 cttgcgcatc gaagaagaac atgcttgtgt agaattgcct taaaatagtc cattcttcat
     1861 tttttattac tggaggtaat tgcagaaggt ctcctataaa aagcacctgt accccgccga
     1921 aagattgttg gttgcgacgg atgtttttca atagaagatc gatagcgtcc agcaaatctg
     1981 ctctcagcat actcacttca tcgatgataa gtagttcaat ttcgcgcaat agtttgcgtt
     2041 tgcgctctgc catacgcatg ttgcgcaata aggtgtccgg tgtgttaatc ctgaatcgat
     2101 cgccggcagc ttcaaatccg attttttccg gcgaaaaagc accaaagggc aattgaaaga
     2161 gggaatgcaa tgttacacct ccggcattga ttgcggctat tcctgtaggt gcagcaatta
     2221 ctgctttctt atgcgtttgt tcaatgattc gtttgagaaa tgtagtcttt ccggttccgg
     2281 cttttccggt gagaaaaaga tgtcgggaag aattttgaat aaatcgggca gcaagagagg
     2341 caggatcatt ggtttgcatg gatcaaaaat acgttatcca tttcatcaaa aggaccgaac
     2401 tgtaaaagtg gtgtaataaa aacgattaat atgagaataa cggatttact cacgcagctg
     2461 attggggtga aaaattaact tttcggcagg ctgaatctca atctattaaa aagtcagaga
     2521 ctgaaaatca gcatttaaat cgtctaaatg aaaataactc tggcttaaga tcggcgaaac
     2581 attcaaggac taatat
//
LOCUS       DAOMYG010000441         2592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_15016_length_2592_cov_6.553015, whole genome shotgun sequence.
ACCESSION   DAOMYG010000441 DAOMYG010000000
VERSION     DAOMYG010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15016_length_2592_cov_6.553015"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2592)
                     /locus_tag="PK610_12285"
     CDS             complement(<1..>2592)
                     /locus_tag="PK610_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRJ36762.1"
                     /translation="HSFFPGSDEDFPEMFMSVRKSGKWGMIGPISDLIVRMEYDHLEP
                     AILGQEQEIPVRIITNKKGKWKLLDDLGKPLNSFEYSSLRFVPAFDANGKPQMRFIAS
                     VGGEFRLVRYEQSDLYGMVETIEKNRLVGAKYMLLNADANRISGELCDSVYELSQSGM
                     FFFVRHFKTGLMNYKGEVLLPAVYNRIDFLGDELFELTESGLSGLFYKGEFVLPMDYS
                     GFDWLSDDLIKLRLEDKFGLFSLSKRKVLITPEYDFIYDQGSSHLIIGLNQLSGIADS
                     TGTLLLPAEYDDIDLGPEGFSLGIARVTSEGKAGYFDLEKSKWSSEPQFDLVGNYPEN
                     GKFFRVNCGGVPEINADGSVDHIVGGKWGILNKRGILVVPPVYKAVSVFAEDIGLFRC
                     ETENSVDYINEKGEKIANGNSIWMKSISYRLKVKNENPGYSFNLLNGPTGADATAFYC
                     DKKGNYWLGTGSSGGVYFSSDNGLNWTAANQGIGPVHVVLLFGRRDTLYSIITESGDY
                     FDGENPAMISRVVYRMESDKSWNYLFAEKNEESTSQEIVFSDHLIRDYLSAGLPGLWF
                     METEHFFSGGEKLVSRYSTMQTKGWLSGNKQDTLFENWPYDLMLRIGGNIHFAGDDTL
                     LLAVSGLYRVKNNTVVPVSEKGLIASDITQMEWSPDGSLILREGEHDIWRYRNGSFVK
                     LIDNYKWNLTRPKNMTRRNTGRFSIDKNGLVYVVVADQLVQIAADNTVSILAGSALMH
                     ELFNDDSYYYNVLARPLFAFRDSNDELIAAFELVSDAEVIPMIYFGVRKNGKKDFVFS
                     DQMKIERSCDELGLHQMGMAKYLISCYSLFGTQESDLLSLPVEEANYFDIPSPGKIAV
                     AQDGTIAV"
BASE COUNT          808 a          632 c          412 g          740 t
ORIGIN      
        1 aaccgcaatc gttccatctt gtgccacagc tatttttcct ggtgatggaa tatcaaaata
       61 atttgcttcc tcaacaggaa gagaaagtaa atctgattct tgagtaccaa acaagctata
      121 gcaggatatt aaatacttag ccatacccat ctgatgcaaa cccaattcat cgcacgaccg
      181 ttcaattttc atttgatcac taaagacaaa atcttttttt ccatttttcc gaacaccaaa
      241 ataaatcatc ggaatcactt cagcatctga taccaattca aatgccgcaa tcagttcgtc
      301 attactgtct ctaaaagcaa acaatggacg agccaataca ttgtagtaat agctgtcgtc
      361 attaaacaat tcatgcatca atgcgctccc ggcaagaatt gagacggtgt tatcagcagc
      421 aatctgaacc agttgatcag caacaaccac atacaccaat ccgtttttat caatggaaaa
      481 acgtccggta tttctgcgcg tcatattttt ggggcgggtt aaattccatt tgtaattatc
      541 aatcagtttt acaaaactac cattgcgata tctccaaata tcatgttcac cttcacggag
      601 aatgagactg ccgtccggac tccattccat ttgcgtaata tccgaagcaa tcagtccttt
      661 ttccgaaacc ggaactacag tattattctt cactctgtac aatccggaaa cagcaagtaa
      721 caaggtatcg tctcctgcga aatgaatatt tccaccaatt cttagcatta aatcataagg
      781 ccagttttcg aaaagcgtat cctgcttatt tccgctgagc catcctttgg tttgcatggt
      841 tgaataacgc gatacaagct tttctcctcc actgaaaaaa tgctctgttt ccataaacca
      901 caatcccggt aagcctgcag ataaatagtc gcggatcaag tgatcggaaa aaacgatctc
      961 ctgactcgta ctttcttcgt tcttttccgc aaaaaggtaa ttccatgatt tatccgattc
     1021 cattcgatac accaccctgc tgatcattgc aggattttcc ccatcgaaat aatcaccgga
     1081 ttccgtaatt attgaataaa gcgtatctct tcgaccaaac agcaaaacaa catgaacagg
     1141 accaataccc tgattcgcag ctgtccaatt cagtccattg tccgaagaga aatatactcc
     1201 accactcgat ccggttccta accagtaatt tcctttttta tcacaataaa atgctgtagc
     1261 atcagcgccg gtaggaccat ttaacagatt aaaggaatat ccgggatttt cattttttac
     1321 ttttagacga taggaaatcg atttcatcca gatggaattt ccatttgcga ttttctcccc
     1381 cttttcattg atataatcaa cactgttttc agtctcacat ctgaacaaac caatatcttc
     1441 agcaaaaacc gaaacagctt tataaaccgg gggcacaacc aggattccac gtttgttgag
     1501 tattccccat tttccaccga caatatgatc aacagaaccg tctgcattga tctccggaac
     1561 accaccacaa ttgactctga aaaactttcc gttttccgga tagtttccta ccagatcaaa
     1621 ttggggttca cttgaccatt tacttttttc caaatcgaaa tagcctgctt ttccctctga
     1681 agttacccgc gcaattccca gagaaaaacc ttcaggcccg agatcgatat catcgtattc
     1741 agctggaaga agaagtgtac ctgttgaatc tgcaataccc gataactgat tcagtccgat
     1801 aatcagatgc gaagaaccct ggtcgtaaat gaaatcgtat tccggagtga tcaatacttt
     1861 tctttttgaa agtgaaaaca aaccaaattt atcctccagt cgcaacttta tcagatcgtc
     1921 acttaaccaa tcgaaaccgg aataatccat tggcaggaca aattctcctt tataaaacaa
     1981 acctgaaagc ccggattcgg tcagttcaaa aagttcatca cctaaaaaat caatacgatt
     2041 ataaacagca ggcaaaagga cttcaccctt gtaattcatc agtcccgttt tgaaatgacg
     2101 aacaaagaag aacattcccg actgactcaa ttcgtaaacc gaatcacaca attctccact
     2161 gattctgttg gcatcagcat tgagtaacat gtattttgct ccgacgagcc gatttttttc
     2221 aatcgtttcc accataccgt acaaatcact ttgctcataa cgtacaaggc ggaattctcc
     2281 tccaacactg gcaataaatc gcatttgcgg ttttccattt gcatcaaaag caggaacaaa
     2341 ccgtaaggag gaatattcaa aggaattgag cggttttcct aagtcatcca gcaatttcca
     2401 tttacccttc ttattggtaa taatcctaac aggaatttct tgttcctgac ccaatattgc
     2461 aggttccaga tgatcgtatt ccattcttac aatcaaatct gaaattggcc caatcattcc
     2521 ccatttaccc gatttgcgaa ccgacataaa catttccgga aagtcttcat cggatcccgg
     2581 aaaaaaagag tg
//
LOCUS       DAOMYG010000442         2588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_15053_length_2588_cov_8.207659, whole genome shotgun sequence.
ACCESSION   DAOMYG010000442 DAOMYG010000000
VERSION     DAOMYG010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15053_length_2588_cov_8.207659"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2214
                     /locus_tag="PK610_12290"
     CDS             <1..2214
                     /locus_tag="PK610_12290"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HRJ36763.1"
                     /translation="GDKIFLDIRVKERPRLAGYSPFGVKKSEWEKVREHLDLYSGRIV
                     TENLIRNTEIVVRNFFVDKGYRNIKVVIDQVMDTTKTNAVILNIRINKGQKVKIGKIE
                     IEGNETLSDGKIRRLLKDTKTVNPIRFWKSSKYIESNFRKEKTAIIDKLNDKGYRDAR
                     IVSDSVYPISDNRIGIKLNIEEGKQYYFRNITWIGNSKYSSGFLDTILGIKKGDVYNQ
                     GQLESRLYMSQTGLDISSLYMDNGYLFFNITPVEVRVENDSIDFEMRITEGKQARVKN
                     VTIRGNTKTNDHVIRREIRTRPGDLFSRNDIIRTQRELSQLGYFNPEKFGVNPVPNPV
                     EGTVDIEYTVEERPSDQVELSGGWGAGRVIGTLGLSFNNFSVRKFFKKGAWQPLPSGD
                     GQQLSLRAQSTGFFYQGYNFSFTEPWLGGKKPNSLSVNVYHTFSSRDNLRRSDPNKSS
                     LKITGMGIGFGKRLKWPDDYFNLYVEFPSYQYYDLQNYVGVFTFSQGYVNNLAMRFSF
                     SRNSQSQPIFPVSGSVLTFSGKITPPYSYFNNKDYSDISDQDRFKWLEYNKWKFTTAH
                     YTSLNKNAKRKLVLAAKTGFGLLLPWNKMVGDSPFERFKLGGSGLSGMNFIFGQEIIA
                     LRGYEDGSLSRPVGDMLIAKYTIELRYPLSLNPNATVYILGFAEAGNTWQKIKNFDPF
                     AVKRSAGAGVRIFLPMFGLLGLDYGWGFDRIDNDPFSKVPNGRFAFTIGMNLGEL"
     gene            2249..>2588
                     /locus_tag="PK610_12295"
     CDS             2249..>2588
                     /locus_tag="PK610_12295"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRJ36764.1"
                     /translation="MKRFLLLSLLFLSGMMAYGQKYAYVNSQQILEKMPEYQEAQRKL
                     DQLSEQWQKEIEEKYADIEKMWQAYQKEKILLPEEERQKREDDIAIKERAVKDLQKQR
                     FGVNGELFQKR"
BASE COUNT          818 a          499 c          548 g          723 t
ORIGIN      
        1 ggagataaaa tctttttaga catacgcgtt aaggaaagac cccgattagc agggtattct
       61 ccttttggtg tgaagaaaag tgaatgggaa aaagtgcgcg agcatttgga tctttactcc
      121 ggaagaattg taacagaaaa cctcatccga aacacggaaa ttgttgtacg gaatttcttt
      181 gtagacaaag gctacagaaa tatcaaggta gtgattgatc aggttatgga caccacaaag
      241 acaaatgcag ttatcctgaa cattcgcatt aacaaaggtc agaaagttaa gatcggtaaa
      301 attgaaatag aaggcaatga aaccttgagt gatggtaaaa tcaggcgcct gttaaaagac
      361 acgaaaactg ttaatccaat tcgtttctgg aaatcttcga aatatattga gtccaatttt
      421 cgtaaggaaa aaacggctat cattgacaaa ttaaacgaca aaggatatcg tgatgcgcga
      481 atcgtttcag attcagttta tcccatttct gataacagaa ttggaattaa attgaacatt
      541 gaagaaggaa aacaatatta tttccgaaat attacatgga ttggaaattc taaatacagt
      601 tcggggtttt tagatacgat attgggaatc aaaaagggag atgtgtacaa tcaaggtcag
      661 cttgaatccc gtttgtacat gtcgcaaacc ggtcttgata tttcttctct ttacatggat
      721 aatggttatt tgtttttcaa tattactccc gttgaagtaa gggttgaaaa cgattccatt
      781 gattttgaaa tgcgcattac tgaaggaaag caagcgcgcg ttaaaaatgt caccattaga
      841 ggaaatacca aaaccaacga ccacgtaatt cgtcgagaga tcagaacacg tccaggcgat
      901 ttgttttcgc gtaacgacat tatcagaaca cagcgggaat tatcgcaatt gggatatttc
      961 aatcccgaaa agtttggtgt taatccggtt ccaaatcctg ttgaaggaac agtagatatt
     1021 gagtacactg tagaagagcg tccttctgat caggttgaat tatccggtgg ttggggtgcg
     1081 ggtcgtgtga tcggaacact tggactttca tttaataatt tctcggtaag aaaattcttc
     1141 aaaaaaggag catggcaacc acttccttca ggtgacggac agcaattatc attgcgtgca
     1201 caatcaaccg gtttcttcta ccagggttac aatttcagtt tcaccgaacc ctggcttggc
     1261 ggtaaaaaac caaattcatt gtcggtaaat gtgtaccaca cgttctcttc gcgcgacaat
     1321 ttaagaagga gcgatcctaa taaatcctca ttaaaaatta ccggtatggg gattggattc
     1381 ggaaaacgac ttaaatggcc tgatgactat ttcaatcttt atgttgaatt ccctagctat
     1441 caatactacg atcttcaaaa ttatgttggc gtatttacct tctcacaagg atacgtgaac
     1501 aaccttgcga tgcgcttttc cttttcaagg aattcccaaa gccaaccaat ttttccagtc
     1561 tccggatctg ttctgacctt cagcggtaag attactccgc cctattcgta cttcaacaac
     1621 aaggattaca gcgacatcag tgatcaggac cgcttcaaat ggctggagta caacaagtgg
     1681 aaattcacca ctgcacatta cacctctcta aataaaaatg caaagcgaaa actcgttctt
     1741 gctgccaaaa cagggtttgg attactgctg ccctggaata aaatggttgg agattcacct
     1801 ttcgaacgct tcaaactggg tggaagcgga ttatccggta tgaactttat ttttggacag
     1861 gaaatcattg cacttcgtgg atatgaggac ggatcgctat ctcgtccggt tggcgatatg
     1921 ctgattgcta aatacaccat tgaattgcgt tatcctcttt ccttaaaccc taacgcaacg
     1981 gtttatattc tcggatttgc agaagcaggt aatacctggc aaaaaattaa aaacttcgac
     2041 ccttttgcgg tcaagcgttc agcaggagca ggtgtgcgta tctttctccc tatgttcgga
     2101 ttgcttggtt tagattacgg atggggcttt gatagaattg ataatgatcc tttctcgaaa
     2161 gtacccaacg gaagatttgc atttaccatt ggcatgaatc ttggtgaatt gtaacgaaac
     2221 agctaatttt cagacctaaa accctaccat gaaacgtttc ctcctccttt cgctactctt
     2281 cctttcagga atgatggcct atggtcagaa atacgcttat gtaaattcgc agcaaattct
     2341 ggaaaaaatg cccgagtatc aggaagctca gcgcaagctg gatcaactct ctgaacaatg
     2401 gcaaaaagag attgaagaaa agtatgccga tattgaaaaa atgtggcagg cttatcaaaa
     2461 agaaaagatt cttttacccg aagaagagcg tcagaaacgt gaagacgata ttgctatcaa
     2521 ggagcgtgca gtaaaggatc ttcaaaaaca acgcttcggt gtgaacgggg aattgtttca
     2581 aaaacgcc
//
LOCUS       DAOMYG010000443         2584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_15081_length_2584_cov_10.510478, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000443 DAOMYG010000000
VERSION     DAOMYG010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15081_length_2584_cov_10.510478"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1185
                     /gene="thiL"
                     /locus_tag="PK610_12300"
     CDS             145..1185
                     /gene="thiL"
                     /locus_tag="PK610_12300"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009422542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRJ36765.1"
                     /translation="MFENKENRTELSELGEFGLIDHLAKHIGLSHPSTLKGIGDDAAV
                     IDQGNGQVTLVTTDMLTEGVHFDLGYVPLKHLGYKAISVNLSDICAMNGRPAQVVVGL
                     GLSNRFSLEAVEELYAGMKLACERYNVDLVGGDTTTSRAGLVLSITAIGTAAKNKVAY
                     RSGAQENDIIILSGDLGGAYMGLQLLEREKEVFKANPSAQPDLEGFDYILERQLKPEP
                     RLDIVELLDSLGIIPTSMIDVSDGLASEILHICKQSGYGADLYIEKIPLDPMTISTAD
                     AFNLEPVTCGMSGGEDYELLFTIKASDFEKLQNNPLITPIGHITGKGERARLITPQKE
                     AIEITAQGWKHF"
     gene            complement(1189..>2584)
                     /locus_tag="PK610_12305"
     CDS             complement(1189..>2584)
                     /locus_tag="PK610_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36766.1"
                     /translation="YDEEQLKRKFKDQAFINQFSIAKNRLYEQILKSLDAYHANSSID
                     AELKKLIHCAEILYKKTLYGQSHKLLKSARRLAERYDKHTTLLEISMWEKMLIEKDNY
                     TETGEKELIEILEQDRLTLAKISNYNEFWSIKSRLFYILNRKGKARSQNELAEFKAII
                     DNVLIKSEDEALFYETKYLYYHIYSAYYFSIGDYENSFLHLKKNVEHIENNTDKFSEE
                     PNVYFSVLTNIIYISSRLKKFGDVAIYLDKLRSLPEKLALKRNEDLDIKLFSSTSSIE
                     LTLYAQTGDFEKGIELIPIIENGLRLYGERINNVRKAYLCLNIAIMYFGERKMSESLK
                     WLNELLNSIEIEKTEDIYCFAQLLNLIVHLELGNQQLVPYALKSTQRYLNLRNRVYKF
                     ENIFLHFINRILKTRDKKEEHMVYTALLDEFRLIGDDNFEKTAFEYFDFDAWAQSKVT
                     GEDFRSIIRKKALI"
BASE COUNT          747 a          535 c          527 g          775 t
ORIGIN      
        1 aatgtccaac ctcaatggtt gaaaccgttt gcaaatttat ttagattaat tctaaattga
       61 atgaaaaatc acaaaaaaat ggcattgtaa gtttcaaagt aatttcctaa ggcttctaat
      121 ggagaggaat cgtagttttg cattatgttc gaaaacaagg aaaaccgcac ggaattatct
      181 gaactcgggg agttcggctt aattgatcac ctcgccaaac atatcggatt gagtcatccg
      241 agcaccctaa aaggcatcgg cgatgatgca gccgttattg accagggaaa cggccaagta
      301 actcttgtca ccacagatat gttaaccgaa ggcgttcatt tcgatttggg ctatgtaccg
      361 cttaagcact tgggatacaa agcaatatcc gtaaacctct ccgatatttg cgcaatgaat
      421 ggtcgtccag cccaggttgt tgttgggcta ggtttatcca atcgattctc tctggaagca
      481 gtagaagaac tgtacgcagg gatgaaactg gcctgcgaac gatacaatgt agacctggtt
      541 ggaggcgata ccactacttc gagagctggc ctggtattat cgatcactgc cattggcact
      601 gcagccaaaa acaaggtagc ctaccgtagc ggagcacagg aaaatgacat tatcattttg
      661 agtggcgatt taggcggtgc atatatggga ttgcaattgt tagagcgtga gaaagaagtt
      721 ttcaaagcga atccatcggc gcaaccggat ctggaaggct tcgattacat ccttgagcga
      781 caacttaaac ctgaaccaag gttagatatt gttgaattac tggattcact ggggattatt
      841 cccacttcca tgatcgacgt tagcgacggt cttgcgtcgg agattctgca catttgcaag
      901 caatccggat acggtgccga cctctacatt gagaaaatcc cactggaccc tatgacgatt
      961 tcaactgcag atgcatttaa tctggagcct gtaacctgcg gaatgagcgg tggtgaagat
     1021 tatgaattgc tatttaccat taaggcaagt gatttcgaaa aactgcaaaa caatcccctg
     1081 ataactccta tcggtcacat cacgggtaaa ggagaacgtg cacgcttaat tactccgcaa
     1141 aaagaagcta tagaaatcac tgcgcaaggg tggaagcact tttaatgctt aaatcagtgc
     1201 cttctttcgt ataatagaac gaaaatcttc tcctgttacc ttgctctgcg cccaggcatc
     1261 aaaatcgaag tattcgaaag ctgttttctc gaaattatca tcaccaatca aacggaattc
     1321 atcaagcaat gccgtgtaca ccatgtgctc ttcctttttg tctctggttt ttaaaatccg
     1381 attgataaaa tgaaggaaaa tgttttcaaa cttgtaaacc cgattacgca aattcaggta
     1441 acgttgggtt gatttaagtg catacggaac caactgctga ttacctaatt cgagatggac
     1501 gatcaggttt aacaattgtg caaagcaata gatatcttct gttttttcaa tttcaatact
     1561 attcagtaat tcgttcaacc atttcagcga ttccgacatt ttccgttcgc caaaatacat
     1621 gatagcaata ttcagacaga gataagcttt gcgtacgttg ttgatccgct caccatatag
     1681 acgcaaccca ttttcaatta ttggaattag ttcaatcccc ttttcaaaat caccggtttg
     1741 agcatatagt gtaagctcta tgcttgatgt gctggaaaat aatttaatat cgagatcctc
     1801 attgcgttta agcgcaagtt tctcgggcaa ggagcgcagt ttatcgaggt aaatagcaac
     1861 atctccaaac ttttttaagc gactcgaaat ataaatgatg ttggtcagca ctgaaaagta
     1921 aacattgggc tcctcgctga atttatcggt gttattctca atatgctcta cattcttttt
     1981 gaggtgcaaa aaactgtttt cataatctcc gatactgaag taataagccg aataaatatg
     2041 ataatagagg tatttcgttt catagaacaa agcctcatcc tcgctcttaa taagcacatt
     2101 atcgatgatc gctttgaatt cggctagttc gttctgacta cgtgccttgc ctttacggtt
     2161 taggatatag aacaacctgc tttttatact ccagaactca ttgtagttcg aaattttagc
     2221 aagagtcagt cgatcctgtt ccagaatctc gatcaattcc ttttctcctg tttctgtgta
     2281 gttatccttt tcaatgagca ttttctccca cattgaaatt tcgaggaggg tagtatgttt
     2341 atcgtaccgt tccgccagcc ttcttgcgct tttgagcaac ttatgacttt gtccgtacaa
     2401 agtcttttta taaagaattt cagcgcaatg aataagcttc tttaattcgg catcgataga
     2461 gctattggca tggtaggcat ccagactctt cagaatttgt tcatagagac ggttctttgc
     2521 tattgagaac tgattgatga aggcctgatc tttgaacttc cttttgagct gctcctcatc
     2581 gtat
//
LOCUS       DAOMYG010000444         2573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_15175_length_2573_cov_8.349484, whole genome shotgun sequence.
ACCESSION   DAOMYG010000444 DAOMYG010000000
VERSION     DAOMYG010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15175_length_2573_cov_8.349484"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1124)
                     /gene="hisB"
                     /locus_tag="PK610_12310"
     CDS             complement(9..1124)
                     /gene="hisB"
                     /locus_tag="PK610_12310"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HRJ36767.1"
                     /translation="MKRLLFIDRDGTIIVEPPVTFQIDTLEQLRFLPGVIGSLAGIRR
                     ETDFEFVMVTNQDGLGTPAYPQAAFDDVQNKMLEILKGEGVEFDAVHIDKHFPHDNAP
                     TRKPGTAMLTDYFDTSRYDLKNSFVIGDRNTDVLLAKNLGCKAIFIRNNDEQDPSLND
                     FVALNTSDWNEVYRFLKLPARKIVHTRKTKETDIKIEIDLDGSGNAVIHTGLGFFDHM
                     LEQIARHGLLDLSIHAKGDLHIDEHHTIEDTGIALGEAFALALGNKRGIERYGFCLPM
                     DDCLAQVAIDFGGRNWIEWEAEFKREKVGDMPTEMFFHFFKSFSDAAKCNLNVKAEGD
                     NEHHKIEAIFKAFAKSIKMAVKHDPDKNILPSTKGVI"
     gene            1202..2272
                     /locus_tag="PK610_12315"
     CDS             1202..2272
                     /locus_tag="PK610_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HRJ36768.1"
                     /translation="MLKASFSKYDLQFLKPSGTSRGILNSKRSWFLKVWDEHEPECIG
                     IGEVGHLEGLSMESQNEVTATLKEVCASPDDPNWLDHALVNVPSVRFALEQALIDLDQ
                     GAGKILFPSSFTEGTTPIAINGLVWMGPIDEMQRQVREKIESGFSCIKLKIGALNFEE
                     EFALLKEIRSEFSEKVLEIRVDANGAFSPEEAMKKLERLATLQLHSIEQPIRQGQQKK
                     MFELCANSPLPIALDEELIGINSPEKKEQLLEQIKPQFIILKPSLLGGIRSCEEWIMA
                     SAERDISMWITSALESNIGLNAIAQWTATLNTNMPQGLGTGQLYSNNIPSPLELRDGH
                     LHYNRDQQWNTAIISENGTAPA"
     gene            2253..>2573
                     /locus_tag="PK610_12320"
     CDS             2253..>2573
                     /locus_tag="PK610_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylbenzoic acid--CoA ligase"
                     /protein_id="HRJ36769.1"
                     /translation="MEQLRLDGKIITAANLHEVGDPFIREFLSLWWSNEDHIIVTTSG
                     STGKPKSIAIKKSSMLASAQATISFFGLHANTKALLCLPAQYIAGRMMLVRAIVGQWE
                     ITSVR"
BASE COUNT          777 a          579 c          516 g          701 t
ORIGIN      
        1 accgggaatt aaatcacccc ctttgtgctt ggcaaaatat ttttgtcggg atcatgcttt
       61 acagccattt taattgattt tgcaaacgct ttaaaaatgg cctcaatttt atgatgttcg
      121 ttatcgcctt ccgctttaac attcaaattg catttggccg catcgctgaa tgatttgaag
      181 aaatgaaaaa acatttccgt tggcatatcc cccacttttt cacgtttgaa ttctgcctcc
      241 cactcgatcc agttacgtcc accaaaatca attgcaacct gagcgagaca atcgtccatt
      301 ggcaaacaaa agccatatcg ctcaatccct cgtttatttc ccaaagcaag tgcaaacgct
      361 tctcccaggg caatacctgt atcttcgata gtgtggtgtt catcaatgtg aagatcgcct
      421 ttcgcatgaa tggacaaatc aagtaatccg tgacgcgcaa tctgttcaag catgtgatca
      481 aaaaatccca atccggtatg aataaccgca tttcctgatc catccaaatc gatttcgatt
      541 tttatatctg tctcttttgt tttacgtgtg tgaacaattt ttctggctgg taatttcaaa
      601 aaccgatata cctcgttcca atcagaagta tttaatgcga caaaatcgtt gagggatgga
      661 tcttgttcat cgttgttacg gataaatatc gctttgcatc cgaggttttt agccaataaa
      721 acatctgtgt tcctatcacc aatcacaaac gaattcttca aatcatatcg gctggtatca
      781 aaatagtcag tcaacatggc tgttcccggc ttacgggtag gcgcgttgtc gtgcgggaag
      841 tgtttgtcga tatgtactgc atcaaactct acgccttcgc cttttagaat ttcaagcatt
      901 ttgttttgaa catcatcaaa agcagcttgc ggataagcag gcgttcccag tccgtcctga
      961 ttggtaacca tcacaaactc aaaatccgtc tccctgcgaa ttcctgccag actcccaatc
     1021 acaccaggta aaaaacgaag ttgctccagc gtatcaatct gaaaagttac gggtggttca
     1081 acaatgatgg tcccgtcgcg atcgatgaat aatagtcgtt tcatttccca aaaatagctc
     1141 cctaaacgca tcattcaatc ataaaatagc gaaaatgccc caacagatgc taaatttgga
     1201 catgctcaaa gcttctttca gcaaatacga tctgcaattc ctcaaacctt cagggacatc
     1261 aaggggtatt ctaaactcca aacgatcatg gtttctgaag gtatgggatg agcatgaacc
     1321 tgaatgtatc ggcataggtg aggtcggaca tttggaaggg ctcagcatgg agtcgcaaaa
     1381 cgaggtgacg gcaacactaa aagaagtttg cgcttcgccc gatgatccca attggcttga
     1441 tcatgcactg gtaaatgtcc catccgttcg tttcgctttg gaacaggcgc taattgatct
     1501 tgatcagggc gcaggtaaaa ttctttttcc ttcttcattt acggaaggaa ctacacccat
     1561 tgccattaac gggttagtgt ggatgggacc cattgatgaa atgcaaagac aagtcaggga
     1621 aaaaattgaa tccggatttt cctgtatcaa actaaaaata ggtgcgctca attttgaaga
     1681 agaatttgcg ttgctcaaag agatccgttc cgagttttcc gaaaaagttc tcgagatccg
     1741 agtagatgcc aatggtgcct tttcgcctga agaagccatg aaaaaactgg aacgactcgc
     1801 aacgcttcag ctccactcca ttgagcaacc catccgtcag ggacaacaaa aaaaaatgtt
     1861 tgaattgtgt gcaaacagtc cactacccat tgcacttgat gaagaactga ttggaataaa
     1921 ttcacccgaa aaaaaagagc aattgcttga acaaataaaa ccacagttca taattcttaa
     1981 accatccctg ctgggaggaa tacgatcatg tgaagaatgg atcatggcat ccgctgaacg
     2041 ggatatcagc atgtggatta cttcagcact tgaatcgaat atcggtttaa atgctatagc
     2101 acagtggaca gccacattaa acaccaatat gccgcaagga ttgggcaccg gacaattgta
     2161 ttccaacaat attccttcac cacttgaatt aagagatggt catctgcatt acaacaggga
     2221 tcaacaatgg aacacagcaa taatttctga aaatggaaca gctccggctt gacggtaaaa
     2281 tcataacagc agcgaacctc catgaagtgg gcgatccatt tattcgtgaa tttttatcgc
     2341 tgtggtggag caacgaagac cacatcattg tgactacttc aggcagtacc ggaaaaccaa
     2401 aatccatagc gataaaaaaa tcatccatgt tagccagcgc gcaggcaacc atttcttttt
     2461 ttggactgca tgcaaatacc aaagcgctgc tctgtttacc tgcacaatat attgcaggaa
     2521 gaatgatgtt ggtgagagca atcgttggcc aatgggaaat tacaagtgtt cgt
//
LOCUS       DAOMYG010000445         2568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_15226_length_2568_cov_9.404696, whole genome shotgun sequence.
ACCESSION   DAOMYG010000445 DAOMYG010000000
VERSION     DAOMYG010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15226_length_2568_cov_9.404696"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /locus_tag="PK610_12325"
     CDS             complement(<1..126)
                     /locus_tag="PK610_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRJ36770.1"
                     /translation="MASTADFKNGLCFRYNTDLVTIMEFLHVKPGKGPAFVRTKLR"
     gene            complement(247..1068)
                     /locus_tag="PK610_12330"
     CDS             complement(247..1068)
                     /locus_tag="PK610_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36771.1"
                     /translation="MKMYFSRSLFLLVLVVTLFSSCKKRRDGVDLIVARDQGTAEACF
                     NDLKNIADEAFSGQLFLYRGAQDTLVHGCATVIRDTSANPKTIVVDFGSANCQCNDGK
                     NRRGKINITYTGLYRDPGTIITLTPDQYFVNDHQLIGTKTVTNEGRNTNNNLWYSIVV
                     NGQVVKPNNGGTITINSTRQREWTEGESTVLNWLDDVYLITGYGNGVASNGEAYTVAI
                     VIPLRKEMSCYHFVSGSIRVLPGSRPERLIDFGAGTCDNSATITVNGTTYPVSLY"
     gene            1223..2074
                     /locus_tag="PK610_12335"
     CDS             1223..2074
                     /locus_tag="PK610_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36772.1"
                     /translation="MRRLLILACIHLMMLSPVFGQHTYKRKNFPLVDLSNGPYKLSGW
                     HVAPGASYTLTRFANREQTLYNYNDTSYSVTFDPAGRLGLYLEAGRFHIFRYGVLFNY
                     MDYSLAFKQIRGKESFTGALMQESSSSTLLTTEGNGSFSHSFVTGNINLNNIWQINDL
                     EFIQQSIGLNLDYRIISGGGQSGNTLLQSPAMPGRFLAQLHYKIGYGIKWTDRFFVIP
                     TLETPILSFATWDNGRSTYNVFSSRYRPIYLTIRFAWLRKRSNDCPPVDINPEDAKKQ
                     KQYQQSQ"
     gene            2071..2553
                     /locus_tag="PK610_12340"
     CDS             2071..2553
                     /locus_tag="PK610_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36773.1"
                     /translation="MRQHLLFFVIGVFIHSSILAQIKYRGEGSVVEAPITISGLQLAG
                     IGYKDLGLKFPGSDTVSNCKITFTRSTIGQMKELKLTLIIRKGIMVQFQINTNDANQV
                     SALADLSTQYYGPPKKSTTTKSVTMSSWETEKNGKIIQTFLILSNNMKKAELVSWVSN
                     "
BASE COUNT          801 a          551 c          512 g          704 t
ORIGIN      
        1 tctgagttta gtacggacaa aggcaggacc tttacccggt ttcacatgaa gaaactccat
       61 aatggtaaca agatccgtat tgtaacggaa acaaagacca ttcttaaaat ctgctgtaga
      121 tgccataggg aatgaattgg agtgcaaaaa taggggaatg caccaaatag ccaaacctgt
      181 ttatcaacga aaaaagggtg tcgcgaaatt cacaacaccc tgcaatatag atacttaaag
      241 tattttctag taaagtgaaa caggataagt ggttccgttt actgttatag tagctgaatt
      301 atcgcaagtt cctgccccga aatcaatcaa tcgttcggga cgtgaaccgg gtaatacacg
      361 gatcgatccg cttacaaagt ggtaacaact catttccttg cgcaaaggaa tcacaattgc
      421 aacagtgtag gcttctccat tggaagcaac accattaccg taccctgtaa tcaggtagac
      481 atcgtccagc caattaagta cggtcgactc accttcggtc cattcgcgct gtcgtgtaga
      541 attgatggta atggttcctc cgttgtttgg ttttacaacc tgaccattta caacaatgga
      601 gtaccacaga ttattattgg tattacgacc ttcatttgtt actgttttgg taccgatcaa
      661 ttgatgatca tttacgaaat attgatccgg agtgagtgta ataatcgtac cgggatcacg
      721 gtataatccg gtgtaagtga tattgatttt accacgtcga ttcttaccat cattgcactg
      781 acaatttgct gaaccgaaat caacaacaat ggtttttgga ttggcactcg tatcccgaat
      841 cacagttgca caaccgtgca ccaaagtatc ctgggctccg cgatataaaa acagttgtcc
      901 gctaaaagcc tcatcagcga tatttttcaa atcattgaaa caagcttcgg cagtaccctg
      961 atcgcgagca actataagat caacaccatc gcgacgcttt ttacaggatg aaaaaagagt
     1021 aacaactaaa acaagcagaa ataacgaacg tgagaaatac attttcataa ggtttcggtt
     1081 tctttaatcc ttgcagtaaa tgtaccaaag agtaaatccg tttccaaaga aatcacctaa
     1141 acctgtttac ctgttgacaa atccgctgaa aaatcgttca gaaccgcact tctcaaggct
     1201 ctattcttag tatttttaaa ccatgagaag gcttttaatt cttgcgtgta ttcatttaat
     1261 gatgttaagt cctgttttcg ggcagcatac ctacaaacgc aaaaatttcc cgcttgtgga
     1321 tctgagcaat ggtccctata aactaagcgg atggcatgta gcccccggag cttcgtatac
     1381 acttacccga tttgcgaaca gggaacaaac cttatataat tataacgaca cctcctacag
     1441 tgtaactttt gatcctgctg gcagactggg actttacctg gaagccggtc ggtttcatat
     1501 attccgttac ggagttttgt tcaattacat ggactactca ctggcgttta agcaaattcg
     1561 cgggaaagaa tcatttaccg gagcccttat gcaggagagc agctcatcga cgctgttaac
     1621 aacagaaggc aacggcagtt tcagccacag cttcgttacc ggaaatatta atctaaataa
     1681 tatctggcag atcaatgatc ttgaattcat tcagcaaagt atcgggctta atctcgatta
     1741 ccgcatcatt agtggtggcg gacaatcagg gaatacactt ttgcaaagtc ctgcgatgcc
     1801 cggaagattt cttgcgcagc tacactataa aataggttat gggattaaat ggacagatcg
     1861 ttttttcgtt attccaactt tagaaacacc cattttatct ttcgcaacat gggataacgg
     1921 ccgttcaact tacaatgttt tcagcagcag ataccgccct atttatctga ctatccgttt
     1981 tgcctggctc agaaaaaggt cgaacgattg cccgccagtg gatattaatc cggaagacgc
     2041 taaaaaacaa aagcaatacc aacaatcaca atgagacaac atctactttt ttttgtgatt
     2101 ggtgtattca ttcactcttc catactagct caaataaaat acagaggcga aggaagtgtt
     2161 gttgaggcgc ccattaccat ttcgggattg caattggcag gaatcggata caaagatctt
     2221 gggcttaaat tcccggggag cgatactgta agtaactgca aaattacatt tactcgttcc
     2281 actatcggtc agatgaagga actgaaacta acattgatca tccgaaaagg tataatggta
     2341 cagtttcaga tcaacaccaa cgatgcaaac caggtaagtg cattggccga cttgtcgacg
     2401 cagtattatg gtccacctaa aaaaagtact actacaaaat cggttacaat gtcatcgtgg
     2461 gaaaccgaaa aaaacggaaa gatcattcaa acctttttga ttctttcgaa caacatgaaa
     2521 aaggctgaat tggtgagctg ggtttccaac tgatcatctc cagcgtaa
//
LOCUS       DAOMYG010000446         2549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_15397_length_2549_cov_10.075381, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000446 DAOMYG010000000
VERSION     DAOMYG010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15397_length_2549_cov_10.075381"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..1057
                     /gene="pseB"
                     /locus_tag="PK610_12345"
     CDS             8..1057
                     /gene="pseB"
                     /locus_tag="PK610_12345"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HRJ36774.1"
                     /translation="MKMKKFYDYSLDLNGKAILVTGGTGSFGKKFVQTVLDQYPKVKR
                     LVIFSRDELKQYEMSLQFPNSKYPCIRFFLGDVRDKDRLVRAMEGIDIVIHAAALKQV
                     PAAEYNPTECIKTNVNGAENIIEACLANQVKHVVALSTDKAAAPINLYGATKLCSDKL
                     FVAANNIKGHRDIRFSVVRYGNVMGSRGSVIPFFLEKKKSGMLPITHEEMSRFNISLE
                     QGVDLVMLAIANSNGGELYVPKLPSYNIMTLAKAIAPNVKTEIVGVRPGEKIHEDMIT
                     PSDALNTLEFEKYFIIYPNNEGWSKMAEGYLSSGKAKKVNYDFGYNSGKNDHWLNEED
                     IRTLIREHIDPDFKP"
     gene            1063..2223
                     /gene="pseC"
                     /locus_tag="PK610_12350"
     CDS             1063..2223
                     /gene="pseC"
                     /locus_tag="PK610_12350"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HRJ36775.1"
                     /translation="METIPYGRQHITQEDIDAVIEVMKSDFLTQGPAIARFEKAFADY
                     VGARYAIAVANGTAALHLSCLALNVHQGTHVITTPNTFAASANCVRYCGGQVSFADIE
                     PNTFLIDPIEVIRLIKNKPEGYFKGIIAVDFAGYPVNMEHLRKIADENNMWIIEDACH
                     APGAWFTASDGQRHLSGGGRFADLSIFSFHPVKHIATGEGGMITTNDEKLYKKLLLLR
                     THGIIRDPEILKENHGGWYYEMQELGYNYRMPDILAALGVSQLKRAESGLRRRVEIAA
                     RYDRELAGIPVKLPHHDKQVGHAWHLYVIRTKKRKALYDYLREQSIFCQVHYIPVHLM
                     PYYREIGHKPGECPHAETYYEQCLSLPMFPSLSDEAQQQVIGAIKTFFEHEN"
     gene            2213..>2549
                     /locus_tag="PK610_12355"
     CDS             2213..>2549
                     /locus_tag="PK610_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36776.1"
                     /translation="MKTKQTDFWSGEFGKEYTDRNSYSDESWDDFYRANWGATKTEMN
                     EECLEGISKEARILEVGCNMGLQLRGFQRMGFTKLYGIELQQYAVNRAHEWLKDVNII
                     QGSGFDIPFR"
BASE COUNT          754 a          530 c          581 g          684 t
ORIGIN      
        1 aattaaaatg aaaatgaaaa aattctacga ttattcgctc gacctcaatg gcaaggctat
       61 tttggtaacc ggtggcaccg gttcattcgg aaagaagttt gtgcaaactg tccttgacca
      121 ataccccaag gtaaagcgac tcgttatctt ttcacgcgat gaattgaagc agtatgaaat
      181 gtcgcttcag tttccgaatt caaaatatcc ttgcatccgt ttctttcttg gtgatgtccg
      241 agacaaagat cgcctggttc gggcaatgga aggaattgat attgtgattc acgcagccgc
      301 attgaagcag gtccctgctg ctgaatacaa tcccacagaa tgcatcaaaa ctaatgtaaa
      361 tggtgccgaa aacataatag aagcttgtct cgccaatcag gtaaaacatg tagttgcgct
      421 ctctaccgat aaagctgccg ctccgattaa cctgtacggt gcaaccaaac tttgttccga
      481 taaacttttt gttgcagcca ataatatcaa gggacatcgt gatatccgtt tttcggtagt
      541 gagatatgga aatgttatgg gctcaagagg ctcagtaatc ccgttttttt tggagaaaaa
      601 gaaaagcgga atgttaccga taacacacga agaaatgagc cgattcaaca tttcgcttga
      661 acaaggggtt gatctggtaa tgctggccat tgcaaattcc aatggtggtg aattgtacgt
      721 tcccaaactt ccgtcctata acatcatgac tttagcaaaa gccattgctc caaatgtaaa
      781 aacagaaatt gttggtgttc gtccaggcga gaagattcat gaagacatga ttacaccaag
      841 tgatgctttg aatacactgg agtttgaaaa atactttatc atttacccaa ataacgaggg
      901 gtggtcaaaa atggcggagg gatatttatc ttcaggaaaa gccaaaaagg tgaattatga
      961 tttcggttac aattccggaa aaaacgatca ctggctcaac gaagaagata tccgtacact
     1021 gatccgtgaa catatcgatc ctgattttaa accttaatgc atatggaaac catcccttac
     1081 ggccggcaac acattacgca ggaagatatc gatgcggtca ttgaggtgat gaaatcggac
     1141 ttcctaaccc agggtcctgc cattgcacgg tttgaaaagg catttgcaga ttatgtcggc
     1201 gcccgatatg ccattgcagt tgccaacgga acagccgcac tacacctttc ctgtcttgcg
     1261 ctcaacgttc accagggaac acatgtcatt actacgccca acacgtttgc tgcatctgca
     1321 aattgcgtaa ggtattgcgg cggacaagtt tcgtttgcag acatcgaacc caatactttt
     1381 ttaattgacc ccattgaagt cattcggctg atcaagaata aacctgaagg atatttcaag
     1441 ggaattatcg ctgtagattt tgccggttat ccggtgaata tggagcatct gcgtaagatt
     1501 gctgatgaga acaatatgtg gatcattgaa gacgcttgtc acgcacccgg tgcctggttt
     1561 accgcttcag atggtcaacg gcacttaagc ggaggcggac gatttgctga tctttctatt
     1621 ttttcttttc atccggtgaa acatatagct acaggagagg gcggaatgat tacaaccaat
     1681 gatgaaaaac tctacaagaa gttgctactt ctccgtactc atggtattat ccgcgatccg
     1741 gaaatactaa aggaaaatca tggtggatgg tattatgaaa tgcaggagct tggatacaat
     1801 taccgtatgc ccgatatact tgcagcactt ggtgtgtctc agttgaaacg cgcggagtcc
     1861 ggtttacgac gtcgtgtgga aattgcagct cgatacgatc gcgaattagc aggaattccg
     1921 gttaaacttc ctcatcatga taagcaggtg ggacatgcat ggcacttgta cgtaatccga
     1981 accaagaagc gcaaagcact ctatgattac ctgcgtgaac aatctatatt ttgccaggta
     2041 cattacattc ccgttcatct gatgccttac tatagagaga tcggacataa acccggagaa
     2101 tgcccccacg ctgaaactta ttatgaacaa tgcctcagtt tacccatgtt cccctcgctt
     2161 tccgacgaag cgcaacaaca ggtaattggt gcaataaaaa cattctttga acatgaaaac
     2221 taaacaaact gatttttgga gtggcgaatt cggtaaggaa tatactgacc gtaattctta
     2281 cagcgatgaa agctgggatg atttttaccg tgccaattgg ggcgcgacga agaccgaaat
     2341 gaacgaagaa tgcctggagg ggatttcaaa agaagcccgt attctggaag taggttgcaa
     2401 tatgggattg cagttgcgcg gttttcagcg aatggggttt acgaaacttt acggaattga
     2461 gctgcaacaa tatgcagtaa atcgcgctca cgagtggctg aaggatgtta atattattca
     2521 aggttcggga tttgatatcc cttttcgtg
//
LOCUS       DAOMYG010000447         2544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_15426_length_2544_cov_11.448775, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000447 DAOMYG010000000
VERSION     DAOMYG010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15426_length_2544_cov_11.448775"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..724)
                     /gene="rsmG"
                     /locus_tag="PK610_12360"
     CDS             complement(98..724)
                     /gene="rsmG"
                     /locus_tag="PK610_12360"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004919831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRJ36777.1"
                     /translation="MNASLIRSHFPDISKEQEEQFNLLFPLYSEWNARINVISRKDME
                     HFYVRHVLHSLGIAKMIRFQPGTRILDVGTGGGFPGLPLAILFPESRFHLVDSIGKKI
                     KVVQEVSAAIGLKNLTAEHKRAEQVNGKFHFVVSRAVTSFEEFLPWVKGKFEKEQFNA
                     LPNGILYLKGGDLSEELKAVKTQYEILELSSVFEDEFFETKKVVHIRC"
     gene            complement(721..2406)
                     /gene="ettA"
                     /locus_tag="PK610_12365"
     CDS             complement(721..2406)
                     /gene="ettA"
                     /locus_tag="PK610_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016479518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HRJ36778.1"
                     /translation="MSDDRKVIFSMVKVNKTTPQGKHILKDIYLSFFYGAKIGIIGAN
                     GSGKSTVLRIIAGLDKSYQGDVVFSPGYTVGHLAQEPELDENKTVKEIVQEGVQSTMD
                     LLNEFEEINNKFGESEYLDNPDKMEKLMNRQAEVQEKLDAVDAWNIDVKLARAMDALQ
                     CPDGDTSIKVLSGGERRRVALCRLLLQQPDVLLLDEPTNHLDAESVLWLEQHLQQYPG
                     TVIAITHDRYFLDNVAGWILELDRGEGIPWKGNYTSWLEQKKKRLEEEEKSESKRQKV
                     LERELDWVRQGAKGRQAKQKARLQNYEKMLSEDSKEKEAKLELYIPPGPRLGNEVITF
                     EHVAKGFGDRLLYEDLNFKLPPAGIVGIIGPNGAGKTTIFRMIMGEEKPDSGTITIGE
                     TVQIAYVDQSHKEIDPNKTVFEVISGGNENVDLGGKMINARAFVSRFNFNGTDQNKKC
                     GVLSGGERNRLHLAMTLKSGSNVLLLDEPTNDLDVNTIRSLEEALENYAGCAVIISHD
                     RWFLDRVCTHILAFEGDASVYSFEGSFTEYEENKRKRLGDTLEKKFRYKKLVK"
BASE COUNT          684 a          582 c          496 g          782 t
ORIGIN      
        1 gatgacctga tgaacgtggg gatgaattac aaaaagttgt gcggactcga gtttgaattt
       61 aacgagctta cagaataaaa tttctgcttt ttgctattca gcaacggatg tgaaccactt
      121 tttttgtttc gaaaaattca tcctcaaata cactactgag ttcaaggatc tcgtattgtg
      181 ttttcactgc ttttagttct tccgaaagat ctccgccttt taaataaaga atgccattag
      241 gcaaagcatt gaattgttct ttttcaaatt ttccttttac ccagggaaga aattcttcaa
      301 agcttgttac agctctgctt actacaaaat gaaatttacc gtttacctgt tctgcacgct
      361 tgtgctcagc agtcaggttt tttaatccga tcgctgcgct gacttcctgt acaactttta
      421 ttttttttcc aatggaatcg accagatgaa aacgtgattc aggaaataaa atggctagtg
      481 gtaatcccgg aaacccacct ccggtaccta catctaaaat gcgtgtaccg ggttggaaac
      541 gaatcatttt tgctatgcca agcgaatgca aaacatggcg cacataaaaa tgttccatgt
      601 ccttacggga aatgacattg atcctggcat tccattcgga ataaagagga aagagcaggt
      661 tgaattgctc ttcctgctcc ttactgatgt cggggaaatg agaacggatc aacgatgcgt
      721 tcattttaca agtttcttgt aacggaattt tttctccaaa gtatcaccca aacgtttacg
      781 cttgttctct tcatattcgg taaacgaacc ttcaaaggaa tacacgcttg catcaccctc
      841 aaatgcgagg atatgcgtac acacacggtc gaggaaccaa cggtcgtgag aaatgattac
      901 tgcacatccc gcatagtttt ccaacgcttc ttccaaggaa cgaatcgtgt tcacatctag
      961 atcgtttgtg ggttcatcga gcagcaatac gttcgaaccg gattttaatg tcatggcaag
     1021 atgcaatcgg ttgcgttctc caccggataa cactccgcac tttttgttct gatcggtacc
     1081 gttaaaattg aaacgggata caaacgcacg tgcattgatc atcttgccac caagatctac
     1141 attttcatta ccgccggaaa tcacttcaaa tacggttttg ttcggatcaa tctctttgtg
     1201 cgactgatcc acatatgcga tctgtaccgt ttctccaatg gtaattgtac cactgtccgg
     1261 tttttcttcg cccatgatca tccggaaaat ggtggtttta ccagcaccgt taggaccaat
     1321 aatcccaacg attccggcag gaggaagttt gaaattcaga tcttcataaa gtaatctgtc
     1381 gccaaatccc ttagcaacat gttcaaaggt gatgacttca ttgccaagac gtggtccggg
     1441 tggaatatag agttcgagct tggcttcttt ttccttgctg tcctcactga gcatcttctc
     1501 gtaattctgt aaacgcgcct tttgtttcgc ctgtcttcct tttgctccct gacgtaccca
     1561 gtcaagctct cgttcgagaa ctttctgacg tttactttcc gatttttctt cttcttcaag
     1621 acgcttcttc ttttgttcca gccatgaagt gtaatttcct ttccagggaa ttccttctcc
     1681 acgatcaagt tcaagaatcc atcctgctac attgtcgagg aagtaacggt cgtgcgtaat
     1741 cgcaataaca gttccgggat attgttgcaa atgctgctcc aaccataata ccgattcagc
     1801 atcgaggtgg ttagtaggtt catcaagtaa gagtacgtcc ggctgctgca ataataagcg
     1861 acacaatgca actcgtcggc gttctcctcc tgataatact ttgatcgaag tatctccgtc
     1921 cggacattgc aatgcatcca ttgctcttgc cagtttaaca tcgatgttcc aggcatcaac
     1981 agcatccagt ttttcctgga cttctgcctg acggttcatc aatttttcca ttttatccgg
     2041 attatccaga tattctgatt cgccgaattt gttgttgatt tcttcaaatt cattgaggag
     2101 atccatagtg ctttgcacac cttcctgcac aatttccttt actgttttgt tttcatccag
     2161 ttcaggctcc tgagcaaggt gaccaaccgt ataaccggga gaaaaaacaa catcaccctg
     2221 atacgattta tccaaaccgg caatgatgcg taacacagta gatttacctg aaccgttggc
     2281 tccgataatt ccaattttcg ctccgtagaa aaaggagagg taaatgtctt ttagaatatg
     2341 cttaccctgt ggtgtggtct tgttgacctt taccatggaa aaaatgactt tgcggtcgtc
     2401 tgacataata gattcaattt gaaccgcaaa gataattgaa taatggccaa ttgctgaaaa
     2461 gaaagcagtt tttgcccgtc aaacaggaat aagcaggtaa cacattatcc cttaaacacc
     2521 aattgaattc agcgggaagt gaaa
//
LOCUS       DAOMYG010000448         2544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_15427_length_2544_cov_8.370430, whole genome shotgun sequence.
ACCESSION   DAOMYG010000448 DAOMYG010000000
VERSION     DAOMYG010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15427_length_2544_cov_8.370430"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /gene="dnaB"
                     /locus_tag="PK610_12370"
     CDS             complement(<1..1164)
                     /gene="dnaB"
                     /locus_tag="PK610_12370"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRJ36779.1"
                     /translation="MVDGGKLPPQALDLEEAVLGAMMLEKGAVNDAIDILKPESFYKE
                     AHQKIFAVVVDLFQKSQPIDILTVTAGLRQKGELDVVGGPYYISQLTNRVASAANVEY
                     HARIISQKHIQRELIRISSETIKNAYEETADVFDLLDQTEGELYKVTQGNIRRDYESM
                     DKLILQAIDEIEKAKGHMDGISGVATGFTELDRVTSGWQRSDMIVLAARPGMGKTAFV
                     LSMARNSAVLFNQPVAVFSLEMSALQLVNRLIASETELQADKLRKGNLAEHELQQLHT
                     RIKKLAEAPIFIDDTPALSIFELRAKARRLVGQHGVKLIIIDYLQLMTAGGEGKGNRE
                     QEISTISRSIKSIAKELDVPIIALSQLSRSVETRGGDLNGFFRTLIFVVLVMAL"
     gene            1400..>2544
                     /locus_tag="PK610_12375"
     CDS             1400..>2544
                     /locus_tag="PK610_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36780.1"
                     /translation="MKVRFLLLFIPLITGSCRKEKDTISPKGKILAPYEFSFFQLPTT
                     LTIQCEASDETKLESIRLQIIDPNGITIASQQFDPQSTTVSFQRNFILSDPYLPSGDY
                     LIKLTVSDGNNTPSTFRTIRIGETPLSVEKVWITQKENSGSRLAHLSGSVIVPDYLST
                     RDICQLELSAREQLLLLRSTTGTLEGLEINGTQQRFDLNSGNFRHVHFDRSKYYFWAG
                     EQSLELRKFNRNGSVSETYGLFPNGYLTTVPVSRLVEEEHYLLLMGKSGNSNNHSITV
                     VDLSTMNSLPAQATGMSVIHAGEYNSTSIVLFGNSGTDLQISQLQLPSMAITSLQTVS
                     NSKIFEVVSRDNDLYFMGTTTGVLVYQRSTNALLNLAGGSTVSLDWDP"
BASE COUNT          713 a          623 c          518 g          690 t
ORIGIN      
        1 cagggccatg acgagaacga cgaagatcaa ggtgcggaag aagccgttca gatctccacc
       61 gcgggtttcg acagaacgac tcagctgcga caatgcaatg ataggcacat ccagttcctt
      121 ggcaatggac ttaatacttc gcgaaatggt actgatttcc tgttcacggt tccccttccc
      181 ttctccacct gcggtcataa gctgtaggta atcgataata atcaatttaa caccatgctg
      241 accaaccaat cggcgcgctt ttgctcgtaa ttcaaatatt gaaagcgcag gagtatcatc
      301 gataaagatg ggcgcttctg ccaatttctt gatgcgtgta tgcaattgct gcaattcgtg
      361 ttctgcaaga tttcctttac gcaatttatc cgcctgcaat tccgtttcgc tggcgataag
      421 acgatttacc aactgtagag ccgacatctc cagagaaaat acagcaaccg gctgattgaa
      481 cagaacggca gaattgcgtg ccatactcaa tacaaaagca gttttaccca tacccggccg
      541 tgcagcaagt acgatcatat ccgaacgttg ccatccggag gtaacacgat cgagttcagt
      601 aaagccggta gcaacaccac tgataccgtc catgtgacct ttcgcctttt caatctcatc
      661 gatcgcctgc agaatgagtt tatccataga ctcgtaatcg cgacggatat tcccttgtgt
      721 taccttgtac aattcacctt ccgtttgatc cagcagatca aaaacatcgg cggtttcttc
      781 gtaggcgttc tttatggttt cagatgagat tctgatcagt tcgcgctgaa tatgtttttg
      841 cgaaataatt cgcgcgtggt attcaacatt ggctgcagag gcaacacggt tggttaattg
      901 tgaaatgtaa taaggaccac caaccacatc aagttcaccc ttctgacgca aaccagcagt
      961 aacagtaaga atatcgatgg gctgtgattt ttgaaaaaga tcaacaacaa cggcgaaaat
     1021 tttctgatgt gcttccttgt aaaaactttc aggtttcagg atgtcaattg catcgtttac
     1081 tgcacccttt tccagcatca tggcacctaa tacagcctcc tccaggtcaa gcgcctgtgg
     1141 tggtagtttt cctccgtcaa ccattatagt agagagagaa cccttcgatt tggggtacgt
     1201 tcgtttggtg tcggtctgac gctctttctg ttcttccata gctcaaaaat accattcccg
     1261 cccctctgcc cgtcatttcc ctttgcggga atcatgaaaa gctatgaaca gcgcttatca
     1321 acaaatgttg atgttttttt tcaattcaac taaagataag gaaatcagcc ccaaaaatca
     1381 taaattggac cataaactta tgaaggtccg atttctttta ttgttcatcc ctttgattac
     1441 aggctcttgc cggaaagaaa aggacaccat ttctcctaag ggaaaaatac tcgcaccgta
     1501 cgaattcagc ttttttcagc ttcccaccac cctaactata caatgcgaag ccagcgacga
     1561 aacaaaactg gaaagcatcc gcttacaaat tattgaccca aacggtatta ccattgccag
     1621 tcagcaattt gatccccaaa gcactactgt aagttttcaa cggaacttta ttctttccga
     1681 cccctattta ccctccggag attacctcat aaagctgaca gtttccgacg gaaataacac
     1741 cccctcaact ttcaggacca tccgaattgg agaaacccca ttaagcgtag aaaaagtctg
     1801 gatcactcaa aaagagaact ccggttcccg tttggcacat ttgagcggtt cggttattgt
     1861 acccgattat ttgtccaccc gcgatatttg ccaactggaa cttagcgcaa gggaacaact
     1921 tctattgtta cgatcaacaa ccggcacgct ggagggattg gaaataaatg gcacccaaca
     1981 acgctttgat ctgaactccg gtaatttcag acatgtgcat tttgaccgat ccaaatatta
     2041 tttctgggca ggcgaacaat ctcttgaact cagaaaattc aaccgcaatg gaagcgtgag
     2101 tgaaacctac ggacttttcc ccaatgggta tttgaccaca gtaccggtca gccgccttgt
     2161 tgaagaagag cattatctgc tgttaatggg caaaagcgga aattcgaaca accattccat
     2221 aactgttgtt gacctcagta ctatgaatag tttacccgct caggcaaccg gaatgtcggt
     2281 tattcatgcc ggtgaataca acagtacaag cattgtgctt tttggcaatt cagggaccga
     2341 tctgcagatt tctcaattgc agcttccttc aatggcaata accagtttgc aaaccgtttc
     2401 gaattccaaa attttcgaag tagttagtcg ggacaatgat ctctatttca tgggaacaac
     2461 taccggtgtt ttggtttacc agagaagcac caatgcattg ctcaatcttg caggtggaag
     2521 caccgttagc ctggactggg atcc
//
LOCUS       DAOMYG010000449         2526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_15584_length_2526_cov_7.575880, whole genome shotgun sequence.
ACCESSION   DAOMYG010000449 DAOMYG010000000
VERSION     DAOMYG010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15584_length_2526_cov_7.575880"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PK610_12380"
     CDS             <1..306
                     /locus_tag="PK610_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36781.1"
                     /translation="KVTAGGDAYSFYECRYKTARFWFSGRKGGPAYLDHAVILNEEVR
                     LLQQIAVGIQEKQIYSMITCEGHPYPSSLVLHNGAGKAIRFSLYNGEISGIELLSDY"
     gene            complement(362..>2526)
                     /locus_tag="PK610_12385"
     CDS             complement(362..>2526)
                     /locus_tag="PK610_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRJ36782.1"
                     /translation="EVFNRQPNYSVSPNERISSFFGENVFNDQAMRQYLPEQVYEKVR
                     EAILEGHQIDRSIADLVAAGMKDWAISKGATHYTHWFQPLTGTTAEKHDSFFEPIIGG
                     EGRSIEKFSGSMLAQQEPDASSFPNGGIRNTFEARGYTAWDPSSPAFLIGKNLCIPTI
                     FISYTGESLDYKMPLLKALHSIDKAATDVCQYFDKNITKVHATLGWEQEYFLVDSALF
                     NARPDLMMTGRALFGHAPAKGQQLEDHYFGSIPERIYAYMREFETESLKLGIPVKTRH
                     NEVAPNQFECAPIFEEANVANDHNQLLMDLMEKIARKHNFRVLFHEKPFSGINGSGKH
                     NNWSLGTDTGKNLLSPGKTPKANMMFLTFFINTIKAIHDNADLLRAAIATASNDHRLG
                     ANEAPPAIISTFIGASLTKVLDELEKNVKSGKMSAEEKTELKLNIGRIPQIMLDNTDR
                     NRTSPFAFTGNKFEFRAVGSSANCGNAMMVLNTIVSNQLKKFKKEVDAQLAKGADKDE
                     AILQTLRKYIVESKKIRFEGNGYGDEWVKEAEKRGLSNLKDTPRALDVWERKEVAKLF
                     EEEGVLTAREIEARHEIELDQYTLKIQIESRLVGDIALTRIVPAGVKYQNILLENLSK
                     QQQVLGAAGKKTTEGLQTLVSDISNHINTIIIKVDEMIEERKKANNISDSKKRAIAYC
                     EKVKPYFDEIRYHADKLEIIVDDELWSLPKFRELLFTK"
BASE COUNT          645 a          542 c          518 g          821 t
ORIGIN      
        1 aaagttacgg caggtggcga tgcttattct ttttatgagt gccgttacaa aaccgcccgt
       61 ttttggtttt ccggacgaaa aggcgggccg gcctatctcg atcacgcagt aattttaaat
      121 gaagaagttc gattgctcca gcaaatagca gtaggaattc aggaaaaaca aatttactcc
      181 atgattacct gcgaaggaca tccttacccg tcttcgcttg tgctgcacaa tggcgccgga
      241 aaagcgatcc gattctcatt gtataacgga gaaatttcag gtattgaatt gttatcagat
      301 tactaaccta aataattgat tttggaataa aaaaaggctg tcgattccga cagccttttt
      361 tttatttggt gaataatagt tctctgaatt tcggaagact ccacaattca tcatccacaa
      421 ttatttccag tttgtcggca tggtaacgga tctcatcaaa atacggcttc actttttcgc
      481 aataagcaat tgcacgtttt ttcgaatcgg agatgttatt cgctttttta cgctcttcaa
      541 tcatctcatc aaccttaata ataattgtgt taatgtgatt gctgatatcg cttaccagcg
      601 tttgaagtcc ttctgtcgtt tttttacctg cagcacccaa tacttgctgt tgtttgctga
      661 ggttttcgag aagaatattc tgatatttta cacccgcagg aacaattcgg gtaagtgcaa
      721 tatcgccaac caatcgcgat tcgatctgaa tttttaaagt gtactggtct aattctattt
      781 catgacgtgc ttcaatttca cgggcagtca gtactccttc ttcttcaaaa agtttagcaa
      841 cttcttttct ctcccaaaca tccaaagcac gtggagtgtc tttcaaattc gataagccac
      901 gtttttctgc ttctttcacc cattcatcgc cgtaaccatt tccttcaaaa cgaatttttt
      961 tggattcaac gatatactta cgcaatgttt gcagaatagc ctcgtcttta tccgctcctt
     1021 tcgcaagttg tgcgtcaact tcttttttga attttttcag ctgattggat acaattgtgt
     1081 taagtaccat cattgcattt ccgcaattcg ctgatgaacc aactgctctg aattcaaatt
     1141 tgtttccggt aaatgcaaat ggcgacgtac ggtttctgtc ggtattgtcg agcatgattt
     1201 gtggaatgcg cccgatattg agtttgagtt ctgttttttc ttcagcactc attttaccgc
     1261 tttttacatt cttctccagc tcatcaagca cttttgtaag tgatgcaccg ataaacgtag
     1321 aaatgattgc aggaggcgct tcatttgcac ctaatcggtg gtcgttggat gctgttgcta
     1381 tagcagctct gagtagatct gcattatcgt gaatggcttt aatggtgtta atgaaaaaag
     1441 tcaaaaacat catgtttgcc tttggtgttt tacccggact aagcagattt tttccggtat
     1501 ccgttccgag tgaccagtta ttgtgtttac cgcttccatt tattcctgaa aatggtttct
     1561 catggaaaag aacacggaaa ttatgtttac gtgcaatctt ctccattaaa tccatcagga
     1621 gttggttgtg gtcattcgca acgttggctt cttcgaatat gggtgcacat tcaaattggt
     1681 ttggcgcaac ttcattgtga cgggttttaa ccggaatgcc caacttcaat gattctgttt
     1741 cgaactcgcg catgtaagca tatatgcgct caggaataga accgaagtag tgatcttcca
     1801 actgttgacc ttttgccggt gcatgaccga acaaagcgcg tccggtcatc attaagtcag
     1861 gacgtgcatt gaacaatgcg gaatcaacca gaaaatattc ctgttcccat ccaagcgtag
     1921 catgcacttt cgtgatgttt ttatcgaagt attgacaaac atctgttgca gctttgtcaa
     1981 tggaatgaag tgccttcagc aatggcattt tataatccag cgattcgccg gtataggaaa
     2041 tgaaaatggt tgggatgcaa agattttttc caatcaaaaa agcaggagag gaggggtccc
     2101 atgctgtgta tccgcgtgct tcgaatgtgt ttcgaatacc accattcggg aaactcgatg
     2161 catccggctc ctgttgtgcg agcatgctgc cgctgaattt ttctatcgaa cgtccttccc
     2221 ctccaatgat gggttcgaaa aaagaatcat gtttctcagc agttgttcca gtaagtggtt
     2281 gaaaccagtg tgtataatgt gtggcccctt tggaaatagc ccagtctttc atccccgcag
     2341 caaccaggtc agcaatactt ctatcgattt gatgtccttc aaggattgct tcacgtactt
     2401 tttcgtaaac ctgctccggt aagtactgtc gcatcgcctg atcattgaat acattttcgc
     2461 caaaaaagga cgagatgcgt tcattcgggc ttaccgagta gttgggctgg cggttaaata
     2521 cctctt
//
LOCUS       DAOMYG010000450         2525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_15590_length_2525_cov_8.142510, whole genome shotgun sequence.
ACCESSION   DAOMYG010000450 DAOMYG010000000
VERSION     DAOMYG010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15590_length_2525_cov_8.142510"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /gene="ttcA"
                     /locus_tag="PK610_12390"
     CDS             complement(<1..596)
                     /gene="ttcA"
                     /locus_tag="PK610_12390"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HRJ36783.1"
                     /translation="MEISDQELMEKTTHRIRKNVWSAVNDFTMIVPGDKIMVCLSGGK
                     DSFTMLDMLLHIRLAMKNSFEIIAVNLDQKQPGFPEHILPEYLQQLDVPYRILERDTY
                     SVVKEKVPEGKTMCSLCSRLRRGILYDAASEMNCTKIALGHHQNDILETFFLNLFFAG
                     KMEAMPAKYRTDDARHVVIRPLAYVREKDIEFYSAQRG"
     gene            686..>2525
                     /locus_tag="PK610_12395"
     CDS             686..>2525
                     /locus_tag="PK610_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ36784.1"
                     /translation="MFIRIVFAFYLSVVYCDLVSAQNQGVINGVVKDKRSQELLAGVS
                     VVVEGTDPLVGTASDENGRFKITVAAGSYNIKASFIGYRTEIKYNVVVTSGNASYLTF
                     ELGEDDEQLEEVVVSTNRTIEVATLVTPLSVQRLSTEEIKSNPGGNFDISRVVQSLPG
                     VAGTSGGGGFRNDIIIRGGAPNENVYYLDGIEIPVINHFSTQGSAGGPSGILNVSFIE
                     DVTLSSSAFDARFDNTLASVFQFRQKEGNPDRVQGNLRLSGTEFASTFEGPMGPKTTF
                     LASARRSYLQFLFKAIDLPIRPNYWDFQTKITHRIDKKTTLTFLGVGAIDEFTFGIPR
                     ETSPEKEYILRSSPNINQWNYTNGVSLKRILENGYYTLALSRNMFNNRIDRFEDRRDG
                     EEAYRITGLTSQEIENKLRFDLTRFYGKWKVSYGAVGQYVKFNNQLYNRIRKEIIDTS
                     GNVIQPAIVINYNSAIDFFRYGAYVQSARTFFSDRFGVSAGVRTDLNSFTNSGNNPLK
                     TLSPRLALSYALSKKWKLNASAGTYYKLPIYTVLGYRDANGVLVNKDNEYIRSTHYVA
                     GTEFLPRQSTRITVEGFYKQYSNYPVSVRDGISLANQGGDFGSIGNK"
BASE COUNT          729 a          486 c          569 g          741 t
ORIGIN      
        1 aagcctcttt gtgctgaata aaattcgatg tccttttccc gaacatatgc caacggacga
       61 atcaccacat gtcgggcatc gtcggtacgg tatttggcag gcattgcttc catttttcct
      121 gcgaaaaaca ggtttaaaaa gaatgtttca aggatgtcgt tctggtgatg accaagggca
      181 attttggtgc aattcatttc agaagccgca tcgtacaata ttccgcggcg aagacgggaa
      241 cataggctgc acatggtttt tccttcaggc accttttctt tcactacgct gtaggtgtca
      301 cgctcaagaa tgcggtacgg tacatcgagt tgttgtaaat attcgggtaa aatatgttcg
      361 ggaaaaccgg gttgtttctg atcgaggttt acagcgatta tctcaaacga atttttcata
      421 gccagacgga tgtggaggag catatccagc attgtaaagc tatctttgcc gccgctcagg
      481 cagaccatga ttttgtcgcc gggaacgatc atggtaaaat catttaccgc tgaccataca
      541 ttcttgcgaa tgcggtgggt ggttttttcc ataagttcct gatcgctgat ttccatggtg
      601 caaaagtaca ttgattcgaa acaggaacaa aatgttaaac ctttaagcaa aacgattgtt
      661 agtcttttcc aaaccatttg taagcatgtt tatccggatt gttttcgctt tttatttgtc
      721 agttgtgtat tgtgaccttg tttctgcaca gaatcaggga gtcattaacg gggttgtaaa
      781 agataaacga tcgcaggaat tgcttgccgg cgtttcggta gtggttgaag gaacagaccc
      841 attggtaggt acagcgagcg atgagaatgg tcgatttaaa ataactgttg cagccggtag
      901 ttataatatc aaggcaagtt ttattgggta ccgaactgag ataaagtata atgttgttgt
      961 aacgagtgga aatgccagct acctcacctt tgaattaggc gaagatgatg aacagcttga
     1021 agaagttgtg gtaagtacca acagaactat agaagtcgcc acactggtaa caccactatc
     1081 tgttcagcga ttaagtacag aagaaattaa aagcaaccca ggtgggaatt ttgatatttc
     1141 ccgtgtagta caaagtcttc cgggtgtggc aggaacatca ggtggtggcg gattcagaaa
     1201 cgacattatc attcgtggtg gtgctccaaa tgaaaacgta tactatctcg atggaataga
     1261 aattcctgta atcaatcatt tttccacgca gggaagcgcg ggtggtccat ccggtattct
     1321 taacgtttct tttatcgaag atgttacttt aagctcaagc gcttttgatg cacggtttga
     1381 caatacccta gcgtcggttt ttcaattccg ccagaaagaa ggtaatccgg atcgcgttca
     1441 aggaaatctt cgtttaagtg gtaccgaatt cgccagtacg tttgaagggc cgatgggacc
     1501 taagactact tttctggctt cggcgcgacg gtcgtatctc caatttttat tcaaagcaat
     1561 cgacttgcca attcgtccca attattggga ttttcaaacc aaaataacgc accgaattga
     1621 caaaaaaacc acactgacat ttcttggtgt tggagctatt gatgagttta cgttcggaat
     1681 tcccagagaa acaagtccgg aaaaggaata cattttgcgt tcatcaccaa atatcaacca
     1741 gtggaattat accaatggag tttcgttaaa acgaatactc gaaaatggtt attatacgct
     1801 tgctttaagt agaaacatgt tcaataaccg catcgatcgt tttgaagatc gacgagatgg
     1861 agaagaggcc tatcgtatta cagggttaac aagtcaggaa attgaaaata aattgcgatt
     1921 tgatcttacc agattctatg ggaaatggaa agtgagttat ggtgcagttg ggcagtatgt
     1981 gaagttcaat aatcaattgt ataatcgtat ccgaaaggaa attatagata catccggaaa
     2041 tgtaattcag ccggcaattg ttatcaatta caattctgca atcgattttt tccgctacgg
     2101 cgcatatgta caatctgcac gtactttttt cagcgatcgc tttggtgttt ctgctggagt
     2161 tcgaacagat ttgaattcat ttaccaattc gggtaacaac ccgctcaaaa ccctttctcc
     2221 gcgattggca ctttcttatg ccttatccaa aaaatggaaa ctcaatgcct ctgcaggaac
     2281 ctattataaa ttacctattt ataccgtttt gggatacagg gatgcaaatg gagttcttgt
     2341 gaacaaggac aacgaataca tccgttccac tcattacgtg gcaggaacag aatttctacc
     2401 tcgccaaagc acaaggatta cggttgaagg attttacaag caatattcca actatcctgt
     2461 ttctgtgcgc gatggaatat ctttggccaa tcagggaggt gattttggta gtattggtaa
     2521 taaag
//
LOCUS       DAOMYG010000451         2511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_15726_length_2511_cov_8.481270, whole genome shotgun sequence.
ACCESSION   DAOMYG010000451 DAOMYG010000000
VERSION     DAOMYG010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15726_length_2511_cov_8.481270"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PK610_12400"
     CDS             complement(<1..599)
                     /locus_tag="PK610_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRJ36785.1"
                     /translation="MGIKAALSKPYAKYIRKKLDRLSAEAVKSQKEQFHMLVQTAEST
                     KFGNDHHFSEIKSHSDWVKNVPVRDYELLKGYMELVLEGQPDILWPGKPLYLCKTSGT
                     TSGTKYIPLTKASMPNHIDSAKYALLSYIAETGNAKFVDGKMIFLQGSPELAMKNGIP
                     LGRLSGIVAHHVPNYLQKNRMPSYTTNCIDDWETKVNAVA"
     gene            complement(605..1498)
                     /locus_tag="PK610_12405"
     CDS             complement(605..1498)
                     /locus_tag="PK610_12405"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRJ36786.1"
                     /translation="MEDLDPKQQRKERRKRVIRRLRLKHRLVIIDETTFAERLSLRLS
                     PMNLFIWLGSLVLFLIIGTTLLIAYTPLKEYIPGYPDGSERRALIDARIKADTLESRL
                     KQYDDYVANIQAILNGERPEDTLSRNPEQINKADVQFTRSQEDSALRARVEENEKYEL
                     RSGKENLYNGGDQLYGVFFFTPLQGTVTQSFNVREKHYGIDISSSSKDGIKSTLDGTV
                     IFSDWTSDGGHEIHVHHGNHLVSVYKHNAVLLKKVGDRVKAGDVIAIVGTSGELSDGP
                     HLHFELWHKGKPVDPQTLLIF"
     gene            1596..>2511
                     /locus_tag="PK610_12410"
     CDS             1596..>2511
                     /locus_tag="PK610_12410"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ36787.1"
                     /translation="MKRNLFLINAFLPLALFSVMVWFSACSTEKNAAVNKGYHNMTAH
                     YNGYFNAREIIKESLKVFREGYKEDYSAILPIYLYADEKAASTLYTDMDRAITKTSTV
                     ITKHSMPNPEKNKNKKEEWCKWIDDNWLVMGQAHFYKREFDAALEKFSYIYKTYKDAD
                     IQYEAQIWMVRTYMEMGNMTEASRILERLQRDANDAEEKVKESKKDKGEKKTRKKPTS
                     KGKKKSNEKEPASFPPRLKRDLIITQADYYIRKKDYEKAAEKLVKGIEMTKKKREKAR
                     LTFILAQLYQAQGKNSLASETYAKIPKMN"
BASE COUNT          732 a          565 c          529 g          685 t
ORIGIN      
        1 gccactgcgt ttacttttgt ttcccagtca tcaatgcagt tggtggtata agacggcatt
       61 ctgtttttct gaagataatt gggaacgtga tgagcaacta ttccggataa tcgacccagg
      121 ggtattccgt ttttcattgc gagttcagga ctcccttgca aaaagatcat ttttccgtcg
      181 acaaactttg cgtttcccgt ttcagctatg taactcaata aggcatattt cgctgaatcg
      241 atatggttgg gcatcgatgc ttttgtaagc ggaatatatt tggttccgct tgttgttccg
      301 cttgtcttac acaaatacaa aggtttgccc ggccataaaa tatctggctg accttccaac
      361 accaattcca tgtacccctt cagtaactcg taatcacgta cgggcacatt tttcacccag
      421 tccgaatggc tctttatttc agaaaaatgg tgatcattac caaacttcgt tgattcggct
      481 gtttgcacca acatgtgaaa ttgctccttt tgagatttga ccgcctctgc tgaaagtcgg
      541 tcaagttttt tccggatgta ttttgcatat ggtttactca gagcggcctt tattcccata
      601 atcgttaaaa aattagcagt gtttgcggat ctaccggctt gcctttatgc caaagttcga
      661 aatgcaaatg cggaccatca cttaattctc cggatgtacc aacaatcgca atcacatcgc
      721 ccgctttaac cctgtcgcca accttcttta gtaataccgc attgtgtttg tacacactta
      781 ccaggtggtt accgtggtgc acatgaatct catgaccccc atcggatgtc caatcggaaa
      841 aaataacggt gccatccagc gtagatttga ttccgtcttt tgatgaagag gaaatatcaa
      901 tcccataatg cttttcgcga acgttaaaag actgggtgac ggttccctgc aatggagtaa
      961 agaaaaacac accatacaac tgatctccgc cgttgtagag gttctccttc cccgatcgta
     1021 attcatactt ttcgttctct tccacccttg cgcgtaaagc cgaatcttcc tgacttcggg
     1081 taaattgcac atctgcttta ttgatctgct cagggttacg acttaaggtg tcttccgggc
     1141 gttctccatt caatatagcc tgaatattag ccacatagtc gtcgtattgc tttagtctcg
     1201 actcaagcgt atctgcttta atccgggcgt caatcaacgc tcttcgttca gagccgtcgg
     1261 gataaccggg gatgtactct tttaaggggg tataggcaat aaggagtgta gtgccaataa
     1321 tcagaaacaa caccagcgat cccagccaga taaaaagatt catgggtgat agtcgcagcg
     1381 aaagccgctc agcaaaggtg gtctcatcaa taatgacaag cctgtgcttg agtcgaagtc
     1441 ggcggataac ccgctttctg cgttctttgc gttgctgttt gggatcgagg tcctccatgg
     1501 cttacaaata tcggaattcc ctcttggcag ggtgatttcc cgaaaaataa aataattttg
     1561 tccgattcga gttatatctc gcacattagt cgctcttgaa aagaaatctg tttctaataa
     1621 atgcattttt gcctctcgct ctttttagcg tgatggtatg gttttcagca tgctcaaccg
     1681 aaaaaaatgc agcggtcaac aagggatacc acaacatgac cgcccattac aacgggtatt
     1741 tcaatgcaag ggaaattatc aaagaatcgt taaaggtctt tcgtgagggt tataaggagg
     1801 attattctgc catcctgccc atctatctct atgccgacga aaaggctgcg tctactcttt
     1861 acaccgacat ggatcgggca attaccaaaa cgtctacggt aattacgaag cactccatgc
     1921 ccaaccctga aaagaacaaa aacaaaaaag aggaatggtg taaatggata gacgacaact
     1981 ggctggtaat gggtcaggct catttttaca aaagggagtt cgatgctgcg cttgaaaagt
     2041 tcagttatat ctataaaacc tacaaggatg ccgacattca gtatgaggct caaatctgga
     2101 tggttcgaac ctatatggaa atgggcaaca tgacggaggc ttcaagaatt ttggaacgct
     2161 tgcagcgcga tgccaatgat gcggaagaaa aagtcaaaga aagtaaaaag gacaagggcg
     2221 agaaaaaaac acgtaaaaaa ccaacttcaa aggggaaaaa gaaaagcaac gaaaaagagc
     2281 cggcatcatt tcctccacgt ttaaaacgtg atttgattat tacccaagcc gattattaca
     2341 tccggaaaaa agactacgaa aaagcagctg aaaaactcgt aaaggggatt gagatgacca
     2401 aaaagaaacg cgaaaaagcg cgcctcacct ttatacttgc ccaattgtat caagcccagg
     2461 gaaaaaactc tcttgcctca gaaacatatg ctaaaattcc aaaaatgaac c
//
LOCUS       DAOMYG010000452         2510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_15739_length_2510_cov_7.300611, whole genome shotgun sequence.
ACCESSION   DAOMYG010000452 DAOMYG010000000
VERSION     DAOMYG010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15739_length_2510_cov_7.300611"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1942
                     /locus_tag="PK610_12415"
     CDS             <1..1942
                     /locus_tag="PK610_12415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRJ36788.1"
                     /translation="GGWVISRFRTQSTARSASTLKNITLELQLEKEKLRAEIAEQTNR
                     LLEEEIQEHKLTQEKLIEIQQFLQGIIGSSIDMIMASGVDMKIDMINTAALRAFGYTE
                     EELIGVHPAVLYSSQNEFEKIQHSLEEQGSFTGEIVNRRKNGETFISYLSAALIKDPS
                     GKIIGSMGVSRDITEIIEAERIVEEQNAKIKAIFENSSNMMMWTMDKDLQITSFNRIF
                     GEVVKSVVGKTVETGDSYQDLLGGLPGPVFSRILRAAFANSFNGKAEETEGPLLGGNE
                     NGAWFEIFLNPILLDSGEIEEISCVAYDITERRKAQIELKISEERNAAMIRALPDLIF
                     RMSKSGEYLDVIFKNRNDLADDPENFIGINILQHFPGEMGQRFLSSILHAITTGEVVQ
                     IEYSAPIRGHFRHFEARYSRINDQEALVVIRDISEKKEAENSLIDSLKEKEILLKEVH
                     HRVKNNLQVISSILNLQSSYVKDENTLQILRESQNRIKSMSFIHESLYQTKMFSSVNF
                     SDYIYDLSKNLVHSYQVFGDLVELDFRLGDVHLNLDQSIPCGLIVNELVSNALKYAFP
                     NGRKGCIRIELREEGKLVMMGVQDNGVGLPEGFDANATETLGLQLVSTLVEQIEGTMT
                     LNSTPGKGTEYLITFEKIELNR"
     gene            1945..>2510
                     /locus_tag="PK610_12420"
     CDS             1945..>2510
                     /locus_tag="PK610_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ36789.1"
                     /translation="MSKTSVLVVEDESIVSKDIQQSLTKLGYNVVGSASTGEKAIALA
                     SELRPDIVLMDIMLKGTITGIDAAAEIKKNEGIPVIFLTAYADESTLAKAKVTEPYGY
                     IIKPFKEIDLHTSIEMALYKHKKESEIVKERDFLYSIVENKESKDYIFVKSNSRLIKL
                     NTKEIYYIEALKDYVVINTLNSRFTIHST"
BASE COUNT          790 a          451 c          594 g          675 t
ORIGIN      
        1 gggcggatgg gtgatcagca gattcagaac ccagtccaca gcacgatcgg ccagcacgct
       61 taaaaatatt acgcttgaat tacagctcga aaaagaaaaa ttgcgggcag aaattgccga
      121 acaaacgaac agactgcttg aagaagaaat tcaggagcat aaattaacgc aggaaaagct
      181 gatagagatt cagcagtttc ttcagggtat tatcggaagc tcgatcgata tgattatggc
      241 atccggtgtg gatatgaaaa ttgatatgat caataccgct gctttacgtg ctttcgggta
      301 tacagaggag gagttgatcg gcgtacatcc tgcagtattg tacagtagtc aaaatgaatt
      361 tgaaaaaata cagcattcac tggaagaaca aggttctttc acaggagaaa ttgtgaaccg
      421 aaggaaaaac ggagaaacat ttatttctta tttatctgcc gctttgatta aggatccttc
      481 aggaaagatt atcgggtcaa tgggtgtatc tcgggatatt actgagatca ttgaagcaga
      541 acgcatcgtt gaagagcaga atgcaaagat caaggctatt ttcgaaaact catcgaatat
      601 gatgatgtgg acgatggata aagatttaca gattacatcg ttcaaccgca tatttggaga
      661 agtggtaaaa agtgttgtag gcaaaactgt tgagaccgga gattcatacc aggatttatt
      721 aggcggatta cccggcccgg tgttctctcg aattcttcgc gcggcatttg ccaattcatt
      781 taacggaaaa gccgaagaga cagaaggtcc attattggga ggaaatgaga atggcgcatg
      841 gtttgagatc tttttaaatc caattcttct tgattcagga gagattgaag aaatcagttg
      901 tgttgcctat gatattactg agcgaagaaa ggcgcagatt gaattaaaga tcagtgaaga
      961 acgaaacgcg gcaatgatac gagcattacc ggatttaata ttcaggatga gtaaaagtgg
     1021 cgaatacctg gatgtgatct ttaaaaaccg gaatgatctg gcggacgacc ctgaaaattt
     1081 tattggaata aacatccttc agcattttcc cggagaaatg ggtcagcgtt ttctcagctc
     1141 tattctacat gccattacta ctggagaagt agtgcaaatt gaatacagtg caccaatacg
     1201 gggtcatttc aggcattttg aagcgcgtta ttcccgtatc aatgatcagg aagctctggt
     1261 ggtaattcgt gatatcagcg agaagaagga agccgaaaac agccttatcg attcacttaa
     1321 ggagaaggag atcctgctta aggaggttca ccacagggtg aagaacaatt tgcaggtaat
     1381 ttcgagcatt cttaatctgc agtcgagtta cgtgaaagat gaaaacacgc tccagatcct
     1441 gcgtgaaagc cagaacagga tcaaatccat gtcgttcatc cacgagagtc tttaccagac
     1501 caagatgttc agttccgtaa atttttcaga ctacatatat gacttatcga aaaatttggt
     1561 acattcatac caggtttttg gcgatttggt cgaactggac tttcgtttgg gcgatgtaca
     1621 ccttaacctc gatcagtcaa ttccctgtgg gttgattgtt aacgagttgg tttctaatgc
     1681 gttaaaatac gctttcccaa atgggcggaa aggctgtatc aggatcgagc tgagggaaga
     1741 gggcaagctt gttatgatgg gtgttcagga caatggtgtt ggtttaccgg aaggctttga
     1801 tgcgaatgca acagagacac tcggtttgca gctggtttct actcttgtag aacaaattga
     1861 aggaaccatg acgctcaatt ccacacccgg taagggcact gaatatttga ttacttttga
     1921 aaaaatcgaa ctaaaccgat gaatatgtcc aagacaagcg tactggtagt tgaagacgaa
     1981 agcattgttt ccaaagacat tcagcagagc ctgactaaac ttggttacaa cgttgtgggt
     2041 tctgcatcta caggcgaaaa ggcaattgca ctggcttctg aattgcgtcc tgatattgtg
     2101 ttgatggaca tcatgctcaa aggcactatc accggaatag atgctgcagc agagattaaa
     2161 aagaacgaag ggattcctgt tatctttctt actgcatatg cagatgaaag cacgcttgcc
     2221 aaggcaaagg tgacagagcc ttatggttat atcattaagc cgtttaaaga aattgatctg
     2281 cacacctcaa ttgagatggc gttgtataag cataagaagg aaagtgagat tgtaaaagaa
     2341 cgtgacttcc tttattctat cgttgagaac aaagaatcga aagactatat tttcgttaaa
     2401 tccaattctc gcctgattaa actcaatacc aaagagattt attatatcga ggcgttgaag
     2461 gactatgtgg tgattaacac tcttaacagt cgttttacaa ttcattccac
//
LOCUS       DAOMYG010000453         2496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_15878_length_2496_cov_8.249898, whole genome shotgun sequence.
ACCESSION   DAOMYG010000453 DAOMYG010000000
VERSION     DAOMYG010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15878_length_2496_cov_8.249898"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PK610_12425"
     CDS             <1..717
                     /locus_tag="PK610_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36790.1"
                     /translation="GKIIAISRNGLLPLTHKLTGAFVPKKDELLNGCETPIGFLKFLR
                     EKTNTHPNLHWSAIIDGIRPYQNQIWANWSIDQKKQFHRHIRPFWEIHRHRIPEESSE
                     LIKKLIENGQLSIRAGRIKGISLTRSDEFILSYLDKKSGNLSAVTTDYIVNCTGSQSD
                     FRKIDSPLIKQLLSDGHVVLNDLQMGILTDESGRLIDNKGNSQPNLFTLGPTRKGQLW
                     ESVALNEIRQQAENISNQII"
     gene            954..>2496
                     /locus_tag="PK610_12430"
     CDS             954..>2496
                     /locus_tag="PK610_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRJ36791.1"
                     /translation="MKTTFKIILLLLLFTTTSFVYAQNTGELLTGTITDSETKEPLIG
                     ASVVIKGNSKGTTTDANGKFVLSNSVGFPVRIIVSYIGYHSQEIEAKVPGENLDIKLK
                     SNNTLIDEITISARRREENAQDVPIPISVVSGAQIEESGAFNVNRIKELVPSVQLYSS
                     NPRNTTLNIRGMGSTFGLTNDGIDPGVGFYVDGVYYARPAATALDFVDVDRIEVLRGP
                     QGTLFGKNTTAGAFNITSRAASFTPGATYELSYGNYGFVQAKGSVTGPLSKKTALRIS
                     FSGTQRDGLIKNIATNSDVNDLNNLGVRTQLLYRVNDNLKITWFADFNRQRPDGYAQV
                     VAGVVTTKRAAYRQFNTIIADLGYSLPTTNPYDRIIDHNTPWKSTNDLGGTSLNIDAK
                     IGKGTLTSTTAWRHWYWGPSNDRDFTGLSVLSLSQAPSRHEQWSQEIRYAGDVTDNIS
                     GVIGVYALGQNLWTSPYHSEESGSAQWRFSQNSTSPLWQTPGLLEGYGIRTYSKLQSF
                     TAAAFA"
BASE COUNT          819 a          526 c          499 g          652 t
ORIGIN      
        1 ggaaagatca ttgccatatc cagaaatgga ctactccccc tgacccataa actaacagga
       61 gcattcgttc caaaaaaaga tgaactattg aatggttgcg aaacgccaat aggttttctg
      121 aagtttttac gcgaaaaaac aaatacccat cccaatttgc attggtcggc aatcattgat
      181 gggattcgtc cttaccaaaa tcaaatttgg gcgaattggt caattgatca aaaaaaacaa
      241 ttccaccggc atatccgtcc attttgggaa attcatcgtc accgtattcc tgaagaatct
      301 tcagaactta taaaaaaact gattgaaaac ggacaactct ctattcgggc aggaagaatt
      361 aaaggaatca gtctgacacg atccgatgag tttatcctct cctacctgga taaaaaatcc
      421 ggaaatctat cggctgttac aacagattac atcgtcaatt gcaccggatc tcaaagtgac
      481 ttcagaaaaa ttgattcacc actcataaag caattgctat ctgacggcca cgttgtattg
      541 aacgacctcc aaatgggaat actgactgat gaaagcggaa gactgattga taataaaggc
      601 aattcacaac ccaatctttt caccttaggt ccaacccgaa aaggtcagtt atgggaaagc
      661 gtagcgctga atgaaattag acagcaggcg gagaatatca gcaatcaaat catttagtct
      721 acgacaaatc aaatagggca aacacacgat tttgttttaa tctataatct acctatacga
      781 tagaatattg gtattattgc atcagattta aaaaaataaa acatgaaacg gtctgcaatt
      841 atgtcgggat tgtataactc atttagggga tttcaccaat ccaattggtt gtgttgccga
      901 atgtgaataa aaaaattcca ttcaattttt taagcaactc aatctaaaaa accatgaaaa
      961 ctacattcaa aatcatactt cttttacttc tctttacgac cactagtttt gtttatgcac
     1021 aaaataccgg cgaactctta acaggaacta taacagattc cgaaaccaaa gaacctctta
     1081 ttggagcctc tgtggtgata aaaggaaatt caaaaggcac aacaactgat gccaacggaa
     1141 aatttgtatt aagcaattca gtaggatttc ctgtcaggat tattgtgtct tacatcggat
     1201 accactcaca ggaaattgaa gcaaaagttc cgggagaaaa cctggatata aagctaaaat
     1261 ccaacaacac ccttattgat gaaattacca ttagcgcacg acgcagggaa gaaaatgctc
     1321 aggatgtacc cattcctatt tccgttgtga gtggtgctca aattgaagag tcaggtgcgt
     1381 tcaatgtaaa ccgcatcaag gaactggttc catcggttca attgtactcc tcaaatccca
     1441 gaaatacaac actcaatatc cggggcatgg ggtcaacatt tggattgacc aatgacggaa
     1501 ttgatcccgg agtcggattt tatgttgacg gggtttatta tgctcgtcct gctgccacag
     1561 cgcttgattt tgttgatgta gaccgtattg aagtattgcg tggtcctcaa ggaacattgt
     1621 ttggaaaaaa cacgaccgcc ggagcattta acatcacatc cagagcggcc agctttacac
     1681 ccggtgcaac ctacgaatta agttatggaa actacggatt tgtacaagct aagggatctg
     1741 taaccggtcc cttgagcaaa aaaacagcgc ttcgaatatc attttccgga acacaacgtg
     1801 atggattaat taaaaatatc gctacaaatt cggatgtaaa tgaccttaac aatttaggag
     1861 taagaacaca actactttat cgtgtaaatg ataatctgaa aatcacctgg tttgccgatt
     1921 tcaatcgcca acgtccggat ggatatgccc aggtagttgc aggagtggta accaccaaaa
     1981 gagctgcata ccgccaattc aacactatca tcgcagacct tggttattcg ttgcctacaa
     2041 caaatcccta cgacagaatt atcgatcaca atacaccgtg gaaatctacc aatgatcttg
     2101 gaggaacatc attgaatatt gatgcgaaaa tcggaaaggg tacgctcact tcaacaactg
     2161 catggcgaca ttggtactgg gggccatcca atgacaggga tttcaccgga ttatctgtat
     2221 tatcactttc gcaagcacct tcgcgtcatg agcaatggtc gcaggaaata cgctatgccg
     2281 gtgatgttac ggataatatc agtggcgtga ttggagtgta cgcattaggc cagaatttat
     2341 ggacctcacc ctatcacagt gaagaatcag gatctgcgca gtggaggttt tcccagaatt
     2401 cgaccagtcc attatggcaa acaccgggtt tgttggaggg atacggaatc agaacgtata
     2461 gtaaacttca gagttttaca gcagcagcct ttgcca
//
LOCUS       DAOMYG010000454         2469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_16152_length_2469_cov_8.430820, whole genome shotgun sequence.
ACCESSION   DAOMYG010000454 DAOMYG010000000
VERSION     DAOMYG010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16152_length_2469_cov_8.430820"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PK610_12435"
     CDS             <1..399
                     /locus_tag="PK610_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRJ36792.1"
                     /translation="AGNVNAQAVKGAVITFDKDEHNYGNIKQGGNGECEFKFTNTGTE
                     PLIIQDAKGSCGCTVPEWPKGAIKPGESAVIKVKYDTKRVGPINKSVTVTSNAVNEGT
                     KVLRIKGTVEAVEGGGAPVRTTDGGTPINN"
     gene            404..784
                     /locus_tag="PK610_12440"
     CDS             404..784
                     /locus_tag="PK610_12440"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRJ36793.1"
                     /translation="MRFFLVLYLLLNSALANAQAAEFSFSDRNLKLAKTPEGKVIQFE
                     YPFTNSGKSPLIITEIKVQCPCTTFEYPKDPIPPGGSGVIRVSFDTAGKIGYQDRTLI
                     VYSNALKSPVELRFRVMVDNKKKG"
     gene            853..2079
                     /locus_tag="PK610_12445"
     CDS             853..2079
                     /locus_tag="PK610_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRJ36794.1"
                     /translation="MPQISSKGQLMPPSPIRKLVPYAEEAKRKGRTVYHLNIGQPDIE
                     TPQIVLDGLKKVDLKVIEYSHSAGNESYRKGLAKYYDKYGIKITHNDIIVTTGGSEAL
                     VFGFMTCLDQGDEVIIPEPFYANYNGFAVMAGVTVKPITASIENGYALPPIEEFEKLI
                     TPKTKAILICNPGNPTGYLYSKKELEALRDIVKKHDLFLFADEVYREFCYDGAQPYSA
                     MNLEGIENNVILVDSVSKRYSMCGARIGALITRNQEVIAAALKFGQARLSPPTFGQIA
                     SEIALDTPEIYFENVVNEYVQRRNILVDGLNKIEGVMCPKPGGAFYAMARLPIDNSDK
                     FCQWMLEEFEFENATVMMAPATGFYASKGLGQNEVRVAYVLNQEALKKSIEILDAALK
                     VYPGRTINVAKKATTV"
     gene            2269..>2469
                     /locus_tag="PK610_12450"
     CDS             2269..>2469
                     /locus_tag="PK610_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36795.1"
                     /translation="MNNQNHIQFLIENIDNACIIIDENLGIKNLNRTGKQFFEESVGI
                     NLYEENNLGDYLSAVSKKKLKKL"
BASE COUNT          791 a          502 c          515 g          661 t
ORIGIN      
        1 gcaggaaatg taaatgccca ggctgttaaa ggcgctgtga ttactttcga taaggacgag
       61 cacaattatg gaaatatcaa gcaaggcggt aacggcgaat gcgaatttaa attcaccaat
      121 accgggactg agccccttat tattcaggat gcaaaaggat cttgcggatg caccgtaccc
      181 gagtggccaa aaggcgctat taagccaggc gagtcagctg tgatcaaggt taagtacgac
      241 acaaagcgtg ttggtccgat caacaaatca gttactgtaa catctaatgc tgttaacgaa
      301 ggcaccaagg ttttgcgaat caaaggtact gttgaagccg ttgaaggtgg aggagctcct
      361 gttcgtacaa cagatggtgg aacaccgata aataactaat tggatgaggt ttttcctcgt
      421 tctatatctt cttctgaact ccgctcttgc caatgcgcag gcagcggagt tcagtttttc
      481 tgaccgtaac ctgaagctgg caaaaacacc tgaaggaaag gttattcaat tcgaataccc
      541 atttaccaat agtggcaagt ctccccttat cataaccgaa attaaggttc agtgcccctg
      601 tacaacattc gagtatccca aggaccctat tccgcccgga ggcagtggag ttataagggt
      661 aagttttgat accgcaggca aaatcggtta ccaggaccga acactgattg tctattcaaa
      721 tgccctgaaa tcgcctgtag aactgagatt cagggtgatg gtcgacaaca aaaagaaagg
      781 ttaatcacct ttaattccct ttgttcgcat cgggaagttt gcgagatttg caactcaaaa
      841 taattattca ccatgccaca gatttcttcc aaaggtcagc tgatgccgcc atcgcctatt
      901 cgtaaacttg tgccctatgc tgaagaggca aaacgcaagg gacgaaccgt ttaccacctc
      961 aacattggtc agcccgacat tgaaactcct caaattgtac tcgacggact gaaaaaagtg
     1021 gatctgaaag tgatcgaata ttcgcacagt gcaggaaacg aatcctaccg caaaggattg
     1081 gcgaaatact atgataaata cggaattaaa atcacgcata acgacattat cgtaacaacc
     1141 ggtggatctg aagcactcgt ttttgggttc atgacttgtc tcgatcaggg cgatgaggta
     1201 attattccgg aaccctttta tgccaactac aatggttttg cagtgatggc cggagtaacg
     1261 gtaaaaccca ttacagcaag tattgaaaat ggctatgcat tacctccaat tgaagagttt
     1321 gaaaaattaa tcactccaaa aaccaaagca attctgatct gtaacccggg caatccaacc
     1381 ggatatctgt attcgaaaaa ggagctcgaa gcattgaggg atattgtgaa aaaacatgat
     1441 ctcttccttt ttgccgatga agtttaccgt gagttttgct acgacggagc acaaccctat
     1501 tcagcaatga accttgaggg cattgaaaac aatgtgattc ttgtggattc tgtttctaaa
     1561 cgttactcca tgtgtggtgc acggatcggt gctcttatta cgcgcaacca ggaagtaatt
     1621 gcagcagccc tcaaatttgg tcaggcacga ttgagtccac caacattcgg tcagattgct
     1681 tctgaaattg ctcttgacac acctgaaatc tatttcgaaa atgtagtgaa tgaatacgta
     1741 caacgcagaa atattctggt tgacggatta aataaaattg aaggagtaat gtgtcccaag
     1801 cccggtggtg cattttacgc aatggcacgc ttacccattg acaattctga taaattctgt
     1861 cagtggatgc ttgaagaatt tgaatttgaa aacgcaactg taatgatggc tccggctacc
     1921 ggattttatg ctagcaaagg tcttggtcag aacgaagtcc gcgttgccta cgtcctcaat
     1981 caggaagcgc tcaaaaaatc aattgaaatt ctggatgctg cattaaaagt gtatccggga
     2041 agaacgatta atgttgcaaa aaaagcaact acggtttaat ccatagcctt tattaaatct
     2101 attaaaatgc tgctataaaa gagcagcatt ttttttacat taaaacccta acctccaatg
     2161 ctttatatga ttcatcaccg tgtttatacg aacaataggc aatggacatt cacatatttc
     2221 ttactattat cttatattac atattgattt gatttccgaa aaaaatagat gaataatcag
     2281 aaccacatcc aattcttaat tgaaaacatt gacaatgcct gcatcattat tgatgaaaac
     2341 ctaggcatta aaaatttaaa tcgaaccgga aaacagtttt ttgaagaatc ggtcgggatt
     2401 aatttatatg aagaaaataa tctaggagat tatctatcgg cagtaagcaa aaagaaatta
     2461 aaaaaatta
//
LOCUS       DAOMYG010000455         2464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_16196_length_2464_cov_9.018265, whole genome shotgun sequence.
ACCESSION   DAOMYG010000455 DAOMYG010000000
VERSION     DAOMYG010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16196_length_2464_cov_9.018265"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PK610_12455"
     CDS             complement(<1..480)
                     /locus_tag="PK610_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013751379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRJ36796.1"
                     /translation="MKKRLVILGAGESGAGSAILGLKQGYDVFVSDKGAIKSRYKTLM
                     DAHDVKWEENAHTEELILNADEVIKSPGIPDKAPIVQKLKEKNIPVLSEIEFAGRYTN
                     AKMICITGSNGKTTTTLLTYHILKRAGLNVGLAGNVGQSFALQVAEKDHDHYVLELSS
                     "
     gene            complement(477..1718)
                     /gene="mraY"
                     /locus_tag="PK610_12460"
     CDS             complement(477..1718)
                     /gene="mraY"
                     /locus_tag="PK610_12460"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRJ36797.1"
                     /translation="MLYYLFSYLDEMDFPGAGVFHYISFRAAMAIITSLIISMLFGKR
                     LINYLQYKQVGETIRDLGLQGQMEKKGTPTMGGIIILMAILIPTLLFAKLQNIYVILM
                     IVSTVWLGTIGFIDDYIKVFKKDKKGLAGKFKILGQLGIGLIVGFTLYFHQDVKIKEK
                     VAIDPDKTYEIVTEEGDLIPCSDANYVWKETRSLKTTIPFVKNNEFDYSWISGSGSIL
                     TMVLFVLIVIVIVTAVSNGANITDGLDGLATGTSAIIGTVLAIFAYLSGNLAFADYLN
                     IMYIPDSGELVVFIAAFVGACIGFLWYNAYPAQVFMGDTGSLALGGIIAVFSIAIRKE
                     LLIPIFCGVFLIENLSVMMQVSYFKYTRKKYGEGRRIFLMSPLHHHYQKKGMHESKIV
                     ARFWIVGIMLAVVSIVTLKLR"
     gene            complement(1718..>2464)
                     /locus_tag="PK610_12465"
     CDS             complement(1718..>2464)
                     /locus_tag="PK610_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRJ36798.1"
                     /translation="DYHGEEMVKDTKARVRTYAIKSMADFRARVVENQFSGLQLNIDG
                     KDLWTKLIGSFNAYNILAVYTVARLLGQDDLTVLTQISTLNAPEGRFQYVKSSNDITA
                     IVDYAHTPDALKNVLNTIRDIRTGNEKVITVVGCGGDRDKGKRPLMAGIACELSDKVI
                     ITSDNPRSEEPEAIIMDMMKGVESDQTRKVMSITNRREAIRAACMMAQPGDILLIAGK
                     GHEKYQEIKGERQHFDDLEEVQNCFKLIGS"
BASE COUNT          720 a          597 c          424 g          723 t
ORIGIN      
        1 actgctcaac tccaacacat aatgatcatg atctttttca gcaacctgca aagcaaaact
       61 ttgtccaaca tttcctgcca atccaacatt taatccggcg cgttttaaaa tatggtaggt
      121 caaaagcgtg gtcgtagttt ttccatttga tcccgttata caaatcattt ttgcgtttgt
      181 atagcgaccg gcaaactcaa tttccgacaa cacaggaata tttttttctt tcaatttctg
      241 aacgatgggt gccttatcag gtattcccgg actttttatc acttcatcag cgttcagaat
      301 caattcttcg gtatgtgcat tctcttccca cttcacatca tgtgcgtcca tcagtgtttt
      361 gtaacgggat ttaatcgcac ccttatccga aacaaagaca tcatagccct gcttcaaacc
      421 aaggattgca gatcctgctc cgctttcacc ggcacctaat atgacgagtc tcttcttcaa
      481 cgaagtttaa gtgttacaat tgatacaact gccagcatga ttccaacgat ccaaaatcgg
      541 gcaacaattt ttgattcatg cattcctttt ttctggtaat ggtgatgcag aggagacatc
      601 aaaaagatgc gtcgtccctc gccgtatttc ttccgggtgt acttgaaata cgacacctgc
      661 atcatcacgg aaagattttc gatcagaaaa actccacaga aaattggtat cagtaattct
      721 ttcctgattg caattgaaaa tacagcaatg attcccccaa gcgcgagcga tccggtatca
      781 cccataaaca cttgcgcagg ataggcgttg taccaaagaa atccgataca agcaccaaca
      841 aatgcagcaa taaaaacaac aagctctccc gaatccggga tgtacatgat gttgagataa
      901 tcggcaaagg caaggttacc cgacaagtaa gcaaaaattg caagcaccgt tccgattatc
      961 gctgatgttc cggttgcaag tccatccagt ccatcagtaa tatttgcacc attcgatact
     1021 gccgtaacaa ttacgattac aatcagcacg aacaaaacca tagtgagtat tgaaccagaa
     1081 ccggaaatcc aggaataatc gaattcattg ttttttacaa atggaattgt ggttttcaga
     1141 gagcgggttt ctttccaaac ataattcgca tcactacaag gaatcaagtc accttcttct
     1201 gtaacaattt cgtaggtctt atccggatcg atcgccactt tctccttgat ctttacatcc
     1261 tgatgaaaat aaagtgtgaa tccaacgatt aatccgattc ccaactgacc aagaatttta
     1321 aacttacctg caagtccctt tttatctttc ttaaaaacct tgatgtaatc atcaataaaa
     1381 ccgattgtcc ccagccatac agtagaaacg atcatcagta tcacataaat attctgcagt
     1441 ttggcaaaca acaaggttgg aataagaata gccatcaaaa tgataatgcc acccatagta
     1501 ggagtcccct ttttctccat ttgcccttgc aaaccaagat cgcggatcgt ctcaccaact
     1561 tgtttatact gcaggtaatt gatcaaccgc ttaccaaaca gcatggaaat aataagtgaa
     1621 gtgatgatcg ccatcgctgc acgaaatgag atgtagtgga acactcccgc tccagggaaa
     1681 tccatttcat cgaggtaact gaacaagtag tataacatca gcttccaatg agtttaaaac
     1741 agttttgtac ttcttcgaga tcatcgaaat gttgtcgttc acctttgatc tcctggtatt
     1801 tttcatgacc ctttccggca ataagtaaaa tatcacccgg ctgcgccatc atacatgctg
     1861 cacgtattgc ttcacggcga ttggtaatgg acatcacttt tcgggtttga tccgattcca
     1921 cacctttcat catatccatg ataattgctt ccggttcttc actgcgggga ttatcggatg
     1981 taataattac tttatcactc agctcacagg caattcccgc catcaacgga cgttttccct
     2041 tatcgcgatc gccaccacaa cctactaccg taatcacctt ttcgtttccg gtacgtatat
     2101 cgcgaatggt attcaataca ttcttcaaag catcaggtgt atgcgcataa tcgacaatgg
     2161 ccgttatgtc gttgctcgat ttcacatatt gaaatcttcc ttcgggtgca ttgagtgtgc
     2221 ttatctgcgt taaaacagtt aaatcatcct gacccaacaa acgcgcaaca gtataaacag
     2281 caagaatgtt ataggcatta aaacttccaa tcagttttgt ccaaagatct tttccatcga
     2341 tgttcagctg taatccgcta aactgatttt ccacaaccct tgcgcgaaaa tcagccatac
     2401 tttttattgc atatgtcctt actcttgcct tggtatcttt caccatttct tcgccgtggt
     2461 aatc
//
LOCUS       DAOMYG010000456         2463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_16203_length_2463_cov_8.523256, whole genome shotgun sequence.
ACCESSION   DAOMYG010000456 DAOMYG010000000
VERSION     DAOMYG010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16203_length_2463_cov_8.523256"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="PK610_12470"
     CDS             <1..1496
                     /locus_tag="PK610_12470"
                     /inference="COORDINATES: protein motif:HMM:NF019880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRJ36799.1"
                     /translation="DSYEDLLKTAEEKGYKLDLLAKCGLIKTREDGKSFDFFRGRVIF
                     PIHNITGKVIAFGARTLKSDKKEPKYLNSPETEIYLKSNILYGISFAKRAIIASDNCY
                     LVEGYTDVISMHQAGIENVVASSGTSLTTGQIRLIKRYTQNITILYDGDPAGIKASFR
                     GIDMILEEGMNVKVLLFPNGEDPDSYARNHSTEEVHAFIRENTQDFILFKTKILLSDA
                     QNDPVKKAALIRELVQSVALVPDAITRSVYVKECSKLFDIPEQTLINELNKFRRTKLS
                     KEAGADLPIADPIEQQAEVQRENETLNELLPQEREIARILLNYGEREIDFEITIEDEE
                     IGPDGAPKTHQEVLRTTVAEFTVHQLTSDGIRLENEDFAAIIEDYALALDAGLILTQQ
                     HFLNDPEGASQAAIDLVISPYILSTKWKEKHLIYTKTEDEKLSLMVRNAVYSFKLKKV
                     MQMIMEIQEQLKGDLQDAIVDQLLEEQKSLFEVKKILSAELGRVIIR"
     gene            1496..>2463
                     /locus_tag="PK610_12475"
     CDS             1496..>2463
                     /locus_tag="PK610_12475"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ36800.1"
                     /translation="MKKRIFQILCALLFTATAFAQSADIAELEKKLKSATSQKEKLEI
                     TVDIAKKTMLLDTERGFSIARKGYDESVKLKDKILECKFLNVLAIGHMFQGENIEARK
                     KFDQALELSRIYKDQAMQEKILGNIGYLYEFQGQIDSALSYYHMSVKLSIARKDTAEI
                     ADCYNGIGAIYTNTGKFDSAQYYLKRSYGLHELKQDTEGKADALFNLGMTYYYTAKKD
                     LALGLFIKTRSIYDSLNLENKSSRVRMSIAQTLFDIGRYDEARKEAYKVLRFFEKMQD
                     AFYIANTHYIIANTFEEQNEDDSSMAHYQISRDVFRESGNTNGLGSA"
BASE COUNT          824 a          507 c          533 g          599 t
ORIGIN      
        1 tggattcgta tgaggacctg ctgaaaaccg ctgaggaaaa aggttataag ctggatctct
       61 tagctaaatg cggactcatc aaaacaagag aagacggcaa gagtttcgac tttttcagag
      121 gccgtgtaat ttttcccatt cacaacatta cagggaaagt aattgcattt ggtgcacgaa
      181 cccttaagag cgacaagaaa gaacccaaat acctcaattc gcctgaaacg gagatttacc
      241 ttaagagtaa tattctttat ggtatttcat ttgcaaaaag agccatcata gccagcgata
      301 attgttacct cgttgaaggc tataccgatg tcatttccat gcatcaggca ggaattgaaa
      361 acgtagttgc ttcgagtgga acatcactta caaccggtca gattcgactc atcaagcgat
      421 acactcaaaa catcaccatc ctttacgatg gcgatcctgc gggaataaaa gcatcgttca
      481 gaggaattga tatgattctt gaggaaggca tgaacgtaaa agttctgctc tttcccaacg
      541 gagaagatcc ggattcgtat gcaagaaatc acagcaccga agaagttcat gcgtttattc
      601 gggagaacac acaggatttt attctcttca agacgaagat cctgcttagt gatgcgcaaa
      661 atgatccggt aaaaaaagcg gcgctgattc gcgaactggt gcaatcagta gcgcttgtgc
      721 ccgacgcaat tacccgttcg gtttacgtta aagaatgcag caagctattc gacattccgg
      781 agcaaacgct cattaacgag ctgaataaat ttcgacgcac caaactgagc aaggaagccg
      841 gtgcagattt acccattgca gatccgattg aacaacaggc agaagttcag cgcgagaatg
      901 aaaccctcaa tgaactttta cctcaggaaa gagaaattgc acgaatactg ctcaattacg
      961 gcgaacgtga aatagatttt gagatcacca tcgaagacga agaaattggt cccgacggcg
     1021 cacccaagac tcaccaggaa gttcttcgca ccactgtggc tgagtttaca gtacatcaac
     1081 tcacaagcga cggcatccgg cttgaaaacg aagacttcgc tgccatcatc gaagattatg
     1141 cccttgcact ggatgccgga ttgatactta cccaacagca ttttctcaac gatcccgaag
     1201 gagcatcgca ggcggcaatt gatctggtga tcagtcctta tattctttct acaaaatgga
     1261 aagaaaaaca tcttatttac actaaaacgg aagatgaaaa attaagtctg atggtgcgaa
     1321 atgcggtata ttctttcaaa ctgaaaaaag tgatgcagat gatcatggaa atacaggaac
     1381 aattgaaagg agatctgcag gatgcgattg ttgatcagtt attggaagaa cagaagagtt
     1441 tatttgaagt aaaaaaaata ctttcagccg aattgggcag ggtaattatt cgttaatgaa
     1501 aaaacgcatt ttccagatcc tttgtgcctt gttgtttacc gcaaccgcat tcgctcaatc
     1561 agccgatatt gctgaactgg aaaagaaatt aaaatcggct acatcccaaa aagagaaact
     1621 cgaaatcact gtcgatatcg ccaaaaaaac gatgttgctc gatactgaac gaggattttc
     1681 cattgcacga aaaggctacg atgagtctgt aaaactgaaa gacaaaattc tcgaatgcaa
     1741 attcttaaat gttctcgcta ttggacacat gttccaggga gaaaatattg aagcaagaaa
     1801 aaaattcgac caggctctgg agctttccag gatctataaa gaccaggcaa tgcaggaaaa
     1861 aatcctgggc aacattggtt acctctacga atttcaggga caaatcgaca gtgcgctgag
     1921 ctattaccac atgtcggtta aactatcgat agcacgaaaa gacaccgcag aaattgccga
     1981 ctgctacaac ggtattggcg caatctatac aaataccggt aagtttgatt ctgcgcaata
     2041 ctatctgaaa agaagttatg gactgcacga gttaaaacag gataccgagg gaaaagcaga
     2101 tgccttgttc aacctgggaa tgacctatta ctacactgca aaaaaagatc ttgctttagg
     2161 attgtttata aagacaagat cgatctacga cagcctgaat cttgagaaca aatcgtcgag
     2221 agtaaggatg agcattgcgc aaactttgtt cgacattggc cgatacgatg aagcgcgaaa
     2281 agaagcctat aaagtattgc ggttctttga aaaaatgcag gatgcttttt atatcgcaaa
     2341 tacacattat atcatcgcga atacttttga agaacaaaac gaggatgatt cctcaatggc
     2401 ccattaccag atttcgcgtg atgtatttcg ggaatcagga aacacaaacg gacttggatc
     2461 agc
//
LOCUS       DAOMYG010000457         2452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_16301_length_2452_cov_6.617021, whole genome shotgun sequence.
ACCESSION   DAOMYG010000457 DAOMYG010000000
VERSION     DAOMYG010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16301_length_2452_cov_6.617021"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /gene="holA"
                     /locus_tag="PK610_12480"
     CDS             complement(<1..673)
                     /gene="holA"
                     /locus_tag="PK610_12480"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRJ36801.1"
                     /translation="MSKKSAVDQFEEIMRDLKNKIYHPVYFFCGEEPFYIDLLSDYMQ
                     ENILDEMEREFNQTVVYGQDISEGDLIATCKRYPMMAEYQVVIVKEAQNMKKIEELKS
                     YVENPVSSTILVLCHKYKTVDRRKEFGKMLDKKTVYYVSEKVRDYKLAEWIEKFVKTQ
                     KMKISPRSAAMLAEFLGNDLAKISNEIGKLKILFPEGTEISDEIIQQNIGISKDYNIF
                     ELQNSL"
     gene            complement(670..1443)
                     /locus_tag="PK610_12485"
     CDS             complement(670..1443)
                     /locus_tag="PK610_12485"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HRJ36802.1"
                     /translation="MKTKPEIVENWLPRYTGVPIEQFGKYILLTNFSNYVHLFAKRTG
                     AEIDGIDKAMPSATSGDITIINFGMGSANAATIMDLLTAVQPKAVLFLGKCGGLKKKN
                     NIGDLILPIAAIRGEGTSNDYFPPEVPALPAFQLQRAISTTIREHDRDYWTGTVYTTN
                     RRVWEHDEDFKEYLRKIRAMAIDMETATLFSTGFANKIPTGALLLVSDQPMVPEGVKT
                     EASDKIVTTHFVDKHLEIGIDSLYELINFGRSIKHLRFE"
     gene            1468..1920
                     /locus_tag="PK610_12490"
     CDS             1468..1920
                     /locus_tag="PK610_12490"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HRJ36803.1"
                     /translation="MESGLLYPSYPFKIKKEAGKPQIFDEFRRKWVALTPEEWVRQHV
                     LVYLKTQKKVPPGLIAVETSITLNGMQRRCDAVVYDVSGQALCIIECKATSVPLSQQE
                     FDQVWNYNRVLNVPMLVLSNGLQQIQARIDSSGKPKFLPELPDFEMFN"
     gene            complement(1957..>2452)
                     /locus_tag="PK610_12495"
     CDS             complement(1957..>2452)
                     /locus_tag="PK610_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36804.1"
                     /translation="DEIHLETLRAEHINVQPLAELADFDVPAVPAIHLGLQKTKRHTW
                     YVQYLFHKLYANGKKQITEKEVEHAFKTILKEQEAIYTGYRNLLTKNQWNLLIGLAKE
                     GEVGKPLSKEFIRKYELGTSSSVQVSLKRLNEQEMIYFDENTWKIYDLYFSEWLSSWI
                     QPSV"
BASE COUNT          575 a          545 c          581 g          751 t
ORIGIN      
        1 caagcgaatt ctgcaattcg aaaatgttgt agtccttgga gatcccgatg ttttgctgaa
       61 tgatctcatc gctgatctct gttccttcgg gaaagaggat ttttagtttg ccaatctcat
      121 tgctgatctt ggccagatcg ttacccaaaa actcggcgag catggcggct gaccgtggcg
      181 agattttcat tttttgtgtc ttcacaaact tctcgatcca ttcggccagt ttgtagtcgc
      241 gcactttctc ggagacgtaa tacactgttt ttttatccag cattttacca aactccttgc
      301 ggcggtctac agttttgtat ttgtggcaga ggacaagaat ggtggagctc acgggatttt
      361 ccacataact ttttagctcc tcaatttttt tcatgttttg cgcttccttc acaatcacca
      421 cctggtattc ggccatcatc ggatagcgtt tacaggtagc aatcagatcg ccttcgctga
      481 tgtcttgtcc gtacaccaca gtctggttga attcgcgttc catttcatcc aggatgtttt
      541 cctgcatgta atccgaaagc agatcaatgt agaacggctc ttcaccgcaa aagaaataaa
      601 ccggatgata gatcttgttt ttgagatcgc gcatgatctc ttcaaactga tcaacggctg
      661 actttttgct cattcgaagc ggaggtgttt gatggaacgc ccgaaattaa tcaactcata
      721 gagagaatcg ataccaatct ccagatgttt atccacgaaa tgcgtggtca caatcttgtc
      781 gctggcttct gtttttactc cctcgggtac cattggctga tcggaaacga gcaggagtgc
      841 tccggtgggt attttattgg caaatcctgt tgaaaacaag gtggccgttt ccatatctat
      901 ggccattgcg cggatttttc ggaggtattc tttgaaatct tcgtcatgct cccaaaccct
      961 tcggttggtg gtgtataccg tcccggtcca gtaatcgcgg tcgtgttcgc ggatggtggt
     1021 ggaaatggcg cgctgcaact ggaaggcggg tagggcaggg acttccggcg ggaaatagtc
     1081 attggaagta ccctcaccac gaatggcggc aataggcaga atgagatcgc cgatgttgtt
     1141 tttctttttg agtcccccac atttccccag gaaaagcacc gctttcggtt gaacagctgt
     1201 aagcagatcc ataattgtag ctgcattggc gcttcccatc ccgaagttaa tgatggtaat
     1261 atcgccggag gttgcgctgg gcatggcttt gtcgataccg tcgatttcgg ctcctgttct
     1321 cttggcaaaa aggtgaacgt agttgctgaa attggtgagc aggatgtatt ttccaaattg
     1381 ctctatagga actccggtgt accttggtaa ccagttttca acgatttcgg gtttggtttt
     1441 catgggatat gtatttttag cctcataatg gaatccggac tcctctatcc atcctatcct
     1501 ttcaaaatta agaaggaagc gggcaagccc caaattttcg atgaattccg tcggaaatgg
     1561 gtggctctta cacctgagga atgggtgcgc cagcatgtgc ttgtttactt gaaaacacag
     1621 aaaaaggttc cacccggact catcgccgtt gaaacctcca ttaccctgaa cgggatgcaa
     1681 cgccggtgcg acgctgttgt ttatgatgtt tccggacaag cactctgcat catcgaatgc
     1741 aaggctacca gcgttcctct cagtcaacag gagttcgatc aggtatggaa ctataaccgg
     1801 gtgctaaatg ttccgatgct cgttctcagc aacggactcc aacagattca ggctcgcatt
     1861 gattcttcag ggaaaccaaa gtttttaccc gaattacctg attttgaaat gtttaattaa
     1921 attatgtaaa ttacgttatg taaattacgt aattttttaa acgctgggtt gtatccatga
     1981 actgagccat tcggaaaagt agagatcgta gattttccag gtgttttcat caaaatagat
     2041 catttcctgt tcattaagtc gcttgagtga tacctgcacc gatgagcttg tacccagttc
     2101 gtatttccgg atgaattctt tggataaggg ttttcctact tctccttctt tggcaaggcc
     2161 gattagtaaa ttccattggt ttttggtcag cagattgcgg taaccggtgt aaatggcttc
     2221 ttgttctttg aggatcgtct taaaggcgtg ttccacttct ttttcggtga tctgtttttt
     2281 tccgttggca tacagcttat gaaaaagata ctgtacgtac caggtgtgcc gtttggtttt
     2341 ttggagtccc agatgaatag caggaacagc cggcacatca aagtcggcaa gctcggccag
     2401 cggttgtacg ttgatgtgct ctgcacgaag cgtctcgagg tgaatttcat cc
//
LOCUS       DAOMYG010000458         2445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_16365_length_2445_cov_10.933891, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000458 DAOMYG010000000
VERSION     DAOMYG010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16365_length_2445_cov_10.933891"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /gene="sufD"
                     /locus_tag="PK610_12500"
     CDS             <1..840
                     /gene="sufD"
                     /locus_tag="PK610_12500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HRJ36805.1"
                     /translation="HIQVKKSVEKPVEVLFLSAGGQSVSVVKNHIEISKSASARFRFL
                     FRSADSSSYLTNSITTIHVSANAQFELDQLQFESPAATHYNRTDVTQHRDSKFSINTF
                     SLDGKLIRNDLNIISEGENTETHMRGLYMVNGQQHVDNHTLMDHRVPNCYSNELYKGV
                     MDGKATGVFNGKVFVRRDAQKINAFQSNANVLLSDDARINSKPELEIYADDVKCSHGS
                     TTGQMDEEALFYLRARGIGEESAKKLLIRAYAGEVLDAISDESFRLYLEELIDSRFHW
                     NNV"
     gene            842..2083
                     /locus_tag="PK610_12505"
     CDS             842..2083
                     /locus_tag="PK610_12505"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HRJ36806.1"
                     /translation="MKDLANTVLSSAEIREQFPVLGISVNGRPLVYLDNGATSQKPKC
                     VIDSLAKYYNEQNANIHRGVHTLSQEATSLFESTRSSLKRFLNAEHEHEIIFTSGTTQ
                     GINLVAHSFGKAFINKGDEVIISALEHHSNIVPWQMMCEDKGAVLRVIPMNNAGELDL
                     SVLASMLSNKTKIIAITHVSNALGTINPVEEIIALAKQYKIPVLIDGAQAVQHQQVDV
                     RKLDCDFYVFSAHKMFGPTGVGVLYGKEKWLNAMPPYMGGGDMIKTVTFEKTEYNELP
                     HKFEAGTPNIADVIAFGESIRFMEEIGFDTIRIREEELLQYATQKLSEIDGLRIFGTA
                     AKKTGVISFNIEGLHPYDVGTILDQLGIAVRTGHHCTQPIMDYYRIPGTVRASFAFYN
                     TMEEVDVLSAGIKKAIKMLKR"
     gene            2089..>2445
                     /locus_tag="PK610_12510"
     CDS             2089..>2445
                     /locus_tag="PK610_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010177877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HRJ36807.1"
                     /translation="MSIKDKESEIVEEFAMFEDWDGKYEYLIDLGKDLPAISAEYKTD
                     DYKIKGCQSQVWLHAELIGGRVRFTADSDAIITKGIIALLVRVFDDETPDAIAEADLQ
                     FINEIGLAQHLSPTRSN"
BASE COUNT          755 a          444 c          553 g          693 t
ORIGIN      
        1 catattcagg ttaaaaaatc agttgaaaaa ccggtagaag ttttgttttt gtcagcaggg
       61 ggtcaatccg tttctgttgt caaaaatcac attgaaattt ctaaaagcgc ctctgcacgt
      121 ttccgctttc tttttcgttc ggctgattct tcatcctatt taaccaatag tataactacc
      181 attcacgttt cagcaaacgc acagtttgaa ttggatcaat tgcaattcga atcgccagca
      241 gccacgcatt acaacagaac tgacgtaaca cagcaccgcg acagtaaatt ttccataaat
      301 actttctctc tcgacggtaa actgatccgt aacgatttga atattatttc cgaaggagaa
      361 aatacggaaa cccatatgcg tggattgtat atggtgaacg ggcaacaaca tgtcgataat
      421 cataccttaa tggatcatcg tgtgcccaat tgctattcca acgagctgta taaaggcgta
      481 atggatggaa aagcaacagg agtattcaat ggaaaagtat ttgttcgtag agatgcgcaa
      541 aaaattaatg cgtttcaatc caatgccaat gtgttgttaa gtgacgatgc acgtatcaat
      601 tcaaaacctg aattggagat ctatgccgat gatgtaaaat gttcgcatgg atctacaacg
      661 ggtcagatgg atgaagaggc attgttttat ttacgcgcca gaggaattgg tgaagagagt
      721 gcaaaaaaat tattgattcg agcctacgcc ggagaagttt tggatgcaat ttcagatgag
      781 tcttttcgct tgtatctgga agagttgatc gatagtcgtt ttcattggaa caatgtataa
      841 tttgaaggac ctagcaaata ccgttttgtc ttcagctgaa atcagggaac aatttcctgt
      901 gcttggtatt agtgtcaatg gcagacctct tgtgtatttg gacaatggcg caacgtcaca
      961 aaagcccaaa tgtgttattg attcgctcgc gaaatactac aatgagcaaa atgcaaatat
     1021 tcacaggggt gtacatacat taagtcagga agccacctcg ctttttgagt ccactcgttc
     1081 ttcgcttaaa cgctttctga atgcagagca cgaacacgag ataatattta catcgggaac
     1141 aacgcaggga atcaatttgg ttgcccattc atttggaaaa gcattcatta acaaaggaga
     1201 tgaagtaatc atttcagcac ttgaacatca ttccaatatc gttccctggc agatgatgtg
     1261 cgaggataaa ggagcagtgt tgagggttat tcctatgaat aatgcgggtg aacttgattt
     1321 atcggtgtta gcgtcgatgc ttagcaataa aacaaaaatt attgcaataa cgcatgtctc
     1381 gaacgcactg ggcacgatta atcctgttga agagatcatt gcattggcaa aacaatataa
     1441 aataccggtt ctcatagatg gtgcacaagc tgtgcaacac cagcaagtgg atgtgcgaaa
     1501 actcgattgc gacttttatg ttttcagcgc acacaaaatg tttgggccta caggtgtcgg
     1561 agttttatac ggaaaggaaa agtggttgaa tgccatgcct ccttatatgg gtggtggcga
     1621 catgattaag acggtgacct ttgaaaaaac agaatacaac gaattacctc ataagtttga
     1681 agcaggcact ccaaacattg cagatgttat tgcttttgga gaatcgattc gttttatgga
     1741 ggaaattgga ttcgatacca ttcgcattcg tgaggaagaa ttattgcaat atgcaaccca
     1801 aaagctttcg gaaattgacg gtttgagaat tttcggaacg gctgcaaaaa aaacgggtgt
     1861 gatctcattt aacattgaag gattgcatcc ttatgatgtt gggactattc tagatcaatt
     1921 gggaatagcg gtgcgcactg gtcatcattg cacgcaaccg attatggact attaccgtat
     1981 tcccgggaca gttcgcgctt cttttgcttt ttacaataca atggaagagg ttgatgttct
     2041 ttctgcggga ataaaaaagg cgattaaaat gctgaaacgc taaacaacat gagcataaag
     2101 gataaagagt cggaaattgt tgaggagttc gccatgtttg aagattggga tggtaaatac
     2161 gaatacctga tcgatcttgg aaaagattta cctgcaatat ccgctgagta taaaacggat
     2221 gattacaaaa taaaaggttg tcagagtcag gtttggctgc atgcggaatt gataggggga
     2281 cgtgtccgct tcactgctga tagcgatgca attattacaa agggtattat tgcattattg
     2341 gttcgtgtgt ttgacgatga aacacctgat gcaattgcag aagcggattt acagtttatc
     2401 aatgaaatcg gcctggccca gcacctttca ccaacacgaa gcaat
//
LOCUS       DAOMYG010000459         2442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_16395_length_2442_cov_8.609552, whole genome shotgun sequence.
ACCESSION   DAOMYG010000459 DAOMYG010000000
VERSION     DAOMYG010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16395_length_2442_cov_8.609552"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="PK610_12515"
     CDS             complement(<1..1158)
                     /locus_tag="PK610_12515"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRJ36808.1"
                     /translation="MITLSTQQLAEIIQGKIIRRDADAEASSPLTDSRVDAVPYSKVF
                     FALKGKNHDGHQFLAELIRKGLRMAVISDPGAAIGIGGCDIILVDDPLRALQRWAAFH
                     RAGSDAEIVGVTGSNGKTIVKEWLYQLLRQEKSIVRSPKSYNSQVGVPLSVLLLQEDH
                     ELAIFEAGISHPGEMIHLEKIIQPQIGILTNIGNAHSENFPDRKSLIKNKLELFRSSE
                     SIIYCSNDRETDEYIKTHFPKAERLDWGTHPEAWLQQKETIREGTFTRIHVKSVVGEF
                     KFRIPFSDQASVDNAMTCCSYLIKKGLEPQRIIAGMEQIAPIEMRLELLPGNENSVII
                     NDSYNSDIVSLSIALDFVQQQKGSQQRTLILSDIQQDKRNDDELYAEVGRLL"
     gene            complement(1155..1736)
                     /locus_tag="PK610_12520"
     CDS             complement(1155..1736)
                     /locus_tag="PK610_12520"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRJ36809.1"
                     /translation="MFLERNNQGRRRGWVEVICGSMFSGKTEELIRRMKRAEFARQKV
                     EIFKPSIDRRYNEQDVVSHEGTTIRSTPVTASSEILLLATDVEVVGIDEAQFFDEELV
                     NVCNQLANNGIRVIAAGLDMDFTGKPFGVMPGLLAIAEYVTKVHAICMECGELATYSH
                     RTSASKDLVLLGETDHYLPLCRTCFNQAREEKE"
     gene            1952..>2442
                     /locus_tag="PK610_12525"
     CDS             1952..>2442
                     /locus_tag="PK610_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36810.1"
                     /translation="MENSPYDSMKDLIQRIQSDLLKMEHGELHADALPGLVDDSRELY
                     ERLVVLRYKALERSADKAGIEPKREIQPEVKEVPTTFKLNITSPVTEPVQTSILDAAK
                     EVVPEQKAPEPPKAESPKIPTTAQHAFQAEVSLNEKISQNQARVSIADKLSKKPIADL
                     RTA"
BASE COUNT          642 a          560 c          527 g          713 t
ORIGIN      
        1 cagcaacctt cctacctccg catacaattc atcgtcgttg cgtttgtctt gttggatatc
       61 ggataaaata agtgttcgtt gctgactccc tttttgctgt tgaacaaaat caagtgcaat
      121 ggaaagtgaa acgatatcgg aattgtagct gtcgttaatt attactgagt tctcattacc
      181 gggcagtaat tccagtcgca tttcaatggg tgcgatctgt tccattcctg caataatgcg
      241 ttggggctcc aatccttttt ttatcagata ggaacagcag gtcattgcat tatcaaccga
      301 tgcctgatcg ctgaagggaa tcctgaattt gaattcccca acaactgatt ttacatgtat
      361 gcgggtgaat gttccttcgc gaatggtttc tttttgttgc aaccatgctt cgggatgtgt
      421 gccccaatcc agacgttccg cttttgggaa atgcgttttt atatattcgt ctgtttccct
      481 gtcgttgctg caataaataa tgctttcact ggagcgaaac aattccagtt tgtttttgat
      541 caaggatttt ctgtcgggaa aattttcgga atgtgcattt ccgatattgg tcaggatccc
      601 gatttggggt tgtatgatct tttcaagatg aatcatctct ccgggatgcg aaatccctgc
      661 ttcaaaaatg gccaactcat ggtcttcctg caacagtaaa accgaaagcg gtacaccaac
      721 ctgtgagttg taacttttcg gacttctcac tatacttttt tcctgtcgaa gcaattgata
      781 cagccattcc tttacaatgg tttttccatt actcccggtt actcccacaa tttctgcatc
      841 gcttcctgct cggtgaaaag ccgcccaacg ctgcaatgca cgcagggggt cgtctaccaa
      901 aatgatatca catcctccaa ttccgattgc tgcaccggga tcggaaatta ctgccatacg
      961 cagtccttta cgaatgagct cggcaagaaa ctgatgtccg tcgtggtttt tcccttttag
     1021 ggcaaaaaaa actttggaat agggaacagc atccacacga ctgtcggtca atggtgagct
     1081 tgcttcggca tctgcatcgc gacgaatgat tttgccctga ataatttcag cgagttgttg
     1141 tgtgctaagg gtaatcattc cttttcctcc ctcgcctggt taaaacatgt cctgcacaag
     1201 ggaagataat gatcggtttc tccaagaaga accaaatctt tacttgcact ggtacggtgc
     1261 gaatacgtag caagttctcc acattccatg cagatggcat gaacttttgt gacatattca
     1321 gcaatggcga gtaaacccgg cattacccca aatggctttc cggtgaaatc catatcgagt
     1381 cctgctgcaa ttacacgtat gccgttattg gcgagttggt tgcagacatt caccaattct
     1441 tcatcaaaaa actgtgcttc atcaatgccc actacttcca catcggtagc caaaagtaaa
     1501 atctcagaag acgcagttac gggagtggat cggatggttg ttccttcatg cgaaacaaca
     1561 tcttgttcgt tatagcgacg atcaatggaa ggtttgaaga tctccacctt ttgccttgcg
     1621 aattccgctc ttttcatgcg acggatcagc tcttcggttt tgccggagaa cattgatccg
     1681 cagatcactt ctacccagcc tctgcgccgg ccttggttgt ttcgttcgag gaacatgaag
     1741 taaaaataag caataattgg ttttaccggt aaggatttag cgttcaggac tgtggaattg
     1801 tgtgccacca cctgagttca ttcagactgc actaacgaat tcaatctata gcaatgactt
     1861 ctttgtattg agggtttgct gttaagaaat tcgtatcagt ccccattcat tcgttttcca
     1921 ttatgggcta tatttaccaa aatcttttcc catggaaaac agtccttacg acagcatgaa
     1981 agatctcatt cagcgcattc agtctgatct tctgaaaatg gagcatggcg agttgcatgc
     2041 agatgcttta cccgggttgg tggatgatag tcgggaattg tatgagcgcc ttgtggtgtt
     2101 gcgatataag gcgctggaga gaagcgccga taaagccggt attgagccaa agcgtgaaat
     2161 acaaccggaa gtgaaggaag taccaactac attcaagttg aatattactt caccggttac
     2221 agaacctgta caaacgagta ttctcgatgc agcaaaagaa gttgttcctg aacaaaaagc
     2281 ccccgaaccg ccaaaggccg aatcgccaaa aatccccaca acggcacagc atgcatttca
     2341 ggcagaagtt tctttgaatg aaaaaatttc ccagaatcag gcccgcgttt ccattgcgga
     2401 taaattatct aaaaagccga ttgctgattt acgaaccgca at
//
LOCUS       DAOMYG010000460         2440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_16419_length_2440_cov_8.371069, whole genome shotgun sequence.
ACCESSION   DAOMYG010000460 DAOMYG010000000
VERSION     DAOMYG010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16419_length_2440_cov_8.371069"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PK610_12530"
     CDS             complement(<1..656)
                     /locus_tag="PK610_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRJ36811.1"
                     /translation="MKNIVVPTDFSTVSLNAVEYAAKLAKHLAARLHLLHVYHVPLVA
                     TDVPVIPNLESVEKESLDRLEKLGNEIQDKHGVVSMIKAEMGFTVDEINEYCDKSKCD
                     LVVLGMRGMSKVAELIVGSTTASYIERGNCPVLVIPADASFKDPKHITFASDLQGLEV
                     HSLDILKTVGLHFKSRINIVNVLKGELLPDYNKSVAGIDLEHYFQSLSHLFFFPEGRD
                     V"
     gene            785..1816
                     /locus_tag="PK610_12535"
     CDS             785..1816
                     /locus_tag="PK610_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HRJ36812.1"
                     /translation="MKQSPIDLRSDTVTRPDGGMLEAMMNAELGDDVFMEDPSVIKLE
                     NELAAMFGKEAGLFCPSGTMTNQIAIRLHTVPGQEVITHKESHVYKYEGGGIMVNSLC
                     SVKLVDGNRGRLNPDDVLLNINNPDDIHLPVTRLVSIEDTANRGGGAIYDPQVIEAIS
                     NVCKEKGFPLHLDGARVFNALVETGCDRKKYGSWFDTISICLSKGLGTPVGSVLIGTK
                     QHIHSARRIRKVLGGGMRQAGVLAAAGSYALKHNIDRLLDDHKRARIIGEMLRKNRFV
                     EEIFPVETNIVVARLHNSMPVERFVSLLREKNVLCVPFGAGLVRMVTHLDFHEDQLNQ
                     LENAIQQIA"
     gene            1846..>2440
                     /locus_tag="PK610_12540"
     CDS             1846..>2440
                     /locus_tag="PK610_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36813.1"
                     /translation="MFSCSNKGEVSTPANSEEVHKEDSISNSTDSSKQVLEDTVIIDK
                     KLSFPEKANAFTLKYKRWYNESGPAKSILPDRFSTDTVIRLALTGNRAVLYGENEVYP
                     TAQLYFYSYSDTISFNNALHNWMNCFGSDCTVIREGEDVKSIKTTPSFTLYGFPEIVH
                     LSWLCEHDANNWDVWIQELKILFGKGKKEISVTCGGPL"
BASE COUNT          730 a          446 c          531 g          733 t
ORIGIN      
        1 acatcacgac cttcaggaaa aaagaaaagg tgacttaatg attgaaaata atgttcaaga
       61 tctattccag caacgctttt attataatcg ggcagtaatt ccccctttag cacatttact
      121 atgttgattc tcgatttaaa atgtaatcca acagttttta aaatatcgag cgaatgtact
      181 tcgagtccct gtaggtcgct tgcaaacgta atatgctttg ggtctttaaa actcgcatcg
      241 gcaggtatta caagtaccgg acaattacca cgttcaatat agcttgctgt cgtagatcca
      301 acaatgagtt cagctacctt cgacattcct ctcattccta aaacaacgag gtcacatttc
      361 gatttatcgc aatattcatt gatttcatca actgtaaatc ccatttctgc cttaatcatc
      421 gataccactc cgtgcttatc ctgaatttca tttccaagtt tttctaatcg gtcaagactt
      481 tccttttcaa cactttccag attgggtatt accggtacat ccgttgcaac caagggaaca
      541 tggtaaacat gcagtaaatg caatcgggcg gccaaatgtt tggcaagttt tgctgcatat
      601 tccacagcat ttaatgaaac agtcgagaaa tcagttggaa cgacaatgtt cttcatagtg
      661 attgaattag gggtatcagg cagaacaaaa atagacctgt catccaccgg tgagtatgac
      721 aggaatcaaa gcaaaaactg acttcagtca gttttattag atttgttcta caatttaata
      781 aaaaatgaaa caatctccaa tcgatttacg aagtgatacg gttacgcgtc ctgatggtgg
      841 aatgcttgaa gcgatgatga atgcagagct tggcgatgat gtatttatgg aagatccttc
      901 tgtgattaaa cttgaaaatg aattggcagc gatgtttggg aaagaggccg gtttgttttg
      961 cccttcagga accatgacca atcaaatcgc tattcgtttg cacacagttc ccggacagga
     1021 agtgatcacc cacaaggaat cgcatgttta caaatacgag ggaggaggca ttatggtgaa
     1081 ttcattgtgc agcgtaaaac ttgttgatgg caatcgtgga cgattaaatc ccgatgatgt
     1141 actgttgaat attaataatc cggatgatat tcatctacct gtaacccgat tggtatctat
     1201 tgaagatact gccaatagag gaggaggagc aatttatgat ccgcaggtaa ttgaggcaat
     1261 ttcaaatgtt tgtaaggaaa agggctttcc gcttcatttg gatggagcaa gggtttttaa
     1321 tgctttggta gaaaccggat gtgatcgcaa aaaatacggt tcctggtttg atactatttc
     1381 tatttgtttg agcaaaggac tcggtacgcc ggttggatct gtgttaatcg gaactaagca
     1441 gcatatccat tcagcaagaa ggattcgtaa ggttttaggg ggaggaatgc gtcaggccgg
     1501 tgtgctggct gcagcaggtt cgtatgccct gaagcataat attgatcgct tattggatga
     1561 tcataagaga gcaagaatta ttggtgaaat gctcaggaaa aacagatttg ttgaagaaat
     1621 ttttccagta gaaacaaata tagttgtagc tcgattgcat aattccatgc cggttgagcg
     1681 ttttgtttca ttactcaggg aaaagaatgt tttgtgtgtt cctttcggtg ccggattggt
     1741 tcgtatggtt acacatttgg attttcatga agatcaatta aatcaattgg agaacgccat
     1801 tcagcaaata gcatgaaaaa ttgtttgatc gtattctgcc tattgatgtt ttcttgttcc
     1861 aacaagggag aggtttcaac acccgctaat agcgaggaag ttcacaagga ggattcaatc
     1921 tcaaattcta ctgattcgtc aaaacaggtc cttgaggata ctgtcattat tgataaaaaa
     1981 ttatccttcc cggaaaaagc aaatgccttt acactgaagt acaaacgctg gtacaatgaa
     2041 tccggcccgg ccaaatctat tctgccggat aggttttcta ctgatacggt tattcgttta
     2101 gcattaaccg gaaaccgtgc tgtactttat ggggaaaatg aagtttatcc caccgcccaa
     2161 ttgtattttt acagttacag cgatacaatc agtttcaaca atgcattgca taattggatg
     2221 aattgttttg ggagcgattg cactgtaatt cgtgagggag aagatgtaaa atccataaaa
     2281 accacgcctt cgtttaccct ttatggtttt cctgaaattg tgcatttgtc gtggttatgc
     2341 gaacatgatg cgaacaactg ggatgtctgg attcaggaat tgaaaatctt attcggaaaa
     2401 ggcaaaaagg aaatatcagt gacgtgcggt gggcctttac
//
LOCUS       DAOMYG010000461         2433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_16503_length_2433_cov_9.405803, whole genome shotgun sequence.
ACCESSION   DAOMYG010000461 DAOMYG010000000
VERSION     DAOMYG010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16503_length_2433_cov_9.405803"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2433)
                     /locus_tag="PK610_12545"
     CDS             complement(<1..>2433)
                     /locus_tag="PK610_12545"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRJ36814.1"
                     /translation="TVYDRKFGWCTDQHLGKEFYGDFGCYDVELNFSSNYVVEATGNL
                     TNREEVLPKDLREKLDIRNFTQPIAEKNISEPIPYDPNQRKTWKYHAENVHDFAFTAD
                     PTYRIGEATWNGVSCISVAQEHHAWKWQNAAEYAAKVIEVYSRDFGMYVYHKMVVADA
                     RDGMEYPMLTLDGGFDPDYRGLLAHEIGHNWFFGQVGNNETYRACLDEGFTQFLTAWA
                     QENIDGDTIQRSRPQNKYTDRFKHYDQVRESAVFNGYLRDAIQGDDAVLNTHSDDFGS
                     ALGHGGGYRHAYYKTASMLYNLQYVLGDDLFLAAMKNYFDQWKIAHPYVEDFRNSIIQ
                     YTKVDLNWFFDQWFETKKTIDYAIVKARYGKEKNKYLVTFRRKGEMQMPIDFVVEGEN
                     DSMYYFHIPNTWFVKQTNATVLPKWTGWGKLNEEYTAEITVPVAIRDIRIDPSHRLAD
                     VDQLNNNMRFPVHYEFDSRIRNNPDIHRYEVFVRPELWYNGYDGLKPGVHVNGNYMNY
                     KHIFDVTLWINTGFGHRIPQSISNYEGVNNEFDPISLRLNYKTGLNKFFGKSNFLFSG
                     SVLDGLKTVNTGIEKWNNKRNIRMYGMFKLMYRKDTADLNYLLYPGEWMADKFNNTLN
                     FGIDKNYEFKGGSGKVNFNLRSTAVGSDYSYGQLSMTSIHRFKIWKTRLSTRLFAQYG
                     TGSAIPSESALFLAGANPEEMMNSKYTRSIGFFPYEWTGNYGASTNHFHAGGGLNLRG
                     YAGYLIPDMDSAGNLSFHYKGNSGTSFSAELDLRDIVKWKPKKLSRYFGLDIYLFTDA
                     GIMAKEMSGKRF"
BASE COUNT          656 a          543 c          480 g          754 t
ORIGIN      
        1 caaaacgctt cccactcatt tctttggcca tgatacctgc gtcggtaaac aggtaaatat
       61 ccagtccaaa atagcggctt agcttttttg gtttccactt tacaatatca cgaagatcca
      121 attccgcgct gaaggatgtg ccgctgtttc ctttgtaatg gaaactcaga ttgcctgcgc
      181 tatccatatc cggaataaga taacctgcat agccgcgaag atttaatcct ccacccgcat
      241 gaaaatgatt ggtggaagct ccgtaattac ctgtccactc gtaagggaaa aatccaatcg
      301 agcgggtgta tttactgttc atcatttctt ccggatttgc tcctgccaaa aacaacgcgc
      361 tttcagaggg aatcgctgac cctgttccgt attgtgcaaa caagcgtgta cttaatcgtg
      421 tcttccagat tttaaaacgg tgaatggagg tcatgcttaa ttgtccgtat gaataatcac
      481 taccaacagc ggtcgaacgt aaattgaaat ttacttttcc cgatccccct ttaaattcat
      541 aatttttatc aattccaaaa ttcaaggtat tgttgaactt gtcggccatc cattctcctg
      601 gatacaacaa atagtttaga tcagcggtgt cttttcgata catcagtttg aacattccgt
      661 acattctgat attgcgttta ttattccatt tttcaatgcc ggtgtttacc gtttttagtc
      721 cgtcaagaac agatcctgaa aatagaaagt tggatttccc aaaaaacttg ttcagtccgg
      781 ttttataatt cagccgaagt gaaatcggat cgaattcatt gttcacgcct tcgtaattgc
      841 taatactttg tggaatgcgg tgaccaaatc ctgtatttat ccataacgtc acatcgaaaa
      901 tgtgcttgta gttcatgtaa tttccattta catgcactcc cggttttaag ccatcgtatc
      961 cgttatacca caattccggc ctgacaaaaa cctcataacg gtgaatgtcg ggattattac
     1021 gaatgcgcga atcaaactca taatgcaccg ggaatcgcat attgttgttt aactgatcaa
     1081 catctgcaag acgatgactg gggtcaatgc ggatgtcgcg tattgcaaca ggtacagtga
     1141 tctcggcggt gtattcctca ttgagtttcc cccaaccggt ccatttagga agaacggttg
     1201 cattggtctg ctttacaaac caggtattcg gaatatggaa ataatacatt gaatcatttt
     1261 caccttctac aacgaaatca ataggcattt gcatttcgcc tttgcgtcgg aaagtaacaa
     1321 ggtatttgtt tttttctttt ccgtatctgg cttttacaat agcgtagtca atggtttttt
     1381 tcgtttcaaa ccactggtca aaaaaccagt tcagatcaac tttggtatac tggataatgc
     1441 tgttcctgaa atcttcaaca taaggatgcg caattttcca ttgatcgaaa tagtttttca
     1501 tggctgccaa aaacagatca tcaccaagca catactgaag attgtacaac atggaggctg
     1561 ttttgtaata agcatgacgg tatccgccgc catgtcccaa tgcgcttccg aaatcatcag
     1621 agtgggtgtt gagcacagcg tcatcacctt gtatcgcatc tctgaggtat ccattaaaaa
     1681 cggcggattc gcgaacctgg tcgtagtgtt tgaaacgatc ggtgtatttg ttttgtggac
     1741 gacttctttg aatagtgtcg ccatcaatgt tttcctgcgc ccacgctgtt aggaattggg
     1801 taaatccttc atcaagacat gcgcgatagg tttcattgtt gccaacctga ccaaaaaacc
     1861 agttgtgacc aatttcatgg gcaagtaatc cgcggtaatc cggatcgaat ccaccatcga
     1921 gagtaagcat gggatattcc attccgtctc tggcatctgc aacaaccatt ttgtggtaca
     1981 catacattcc aaaatcgcgg gaataaacct caattacttt tgctgcgtat tccgctgcat
     2041 tttgccattt ccaggcgtga tgttcctgtg ctacggaaat acagctaact ccattccatg
     2101 ttgcttctcc tatgcgatag gttggatccg ccgtaaatgc aaaatcatgt acattctctg
     2161 catggtattt ccaggtcttc cgttggttag gatcgtaagg aattggttcg ctgatatttt
     2221 tttctgcaat gggttgggtg aaattccgga tatcaagttt ttcccgcaaa tcctttggca
     2281 gaacttcttc gcggttggtg agattacctg ttgcctcaac aacataattc gaagagaagt
     2341 ttaattcaac atcataacaa ccaaaatcac cataaaattc tttgcctaaa tgctgatcgg
     2401 tgcaccagcc gaactttctg tcgtacactg tta
//
LOCUS       DAOMYG010000462         2433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_16504_length_2433_cov_7.687132, whole genome shotgun sequence.
ACCESSION   DAOMYG010000462 DAOMYG010000000
VERSION     DAOMYG010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16504_length_2433_cov_7.687132"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PK610_12550"
     CDS             complement(<1..482)
                     /locus_tag="PK610_12550"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRJ36815.1"
                     /translation="MNAIVTYRNLLLLLFLAAGNCSDVLAQKDTTKKQKTTITWLTFQ
                     QLEDSMRVNPKPIFIDIYAVWCGPCKLMDKRTFTNKYVIREMSQSFYAIKLDGETKDT
                     LRFNGKEYSFKIVRDGKGANALALEVGMESNTLSYPTIVILDKNYKLVYRYPSFLTAE
                     "
     gene            complement(479..>2433)
                     /locus_tag="PK610_12555"
     CDS             complement(479..>2433)
                     /locus_tag="PK610_12555"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ36816.1"
                     /translation="TETLLDEVVVSGTMKEVRLMESPIPVTVISPSFLRKNPAPSLFE
                     SLQTVNGVRPQINCSVCNTGDIHINGMEGPYTMVLIDGMPIVSGLASVYGLYGIPTAL
                     VERIEIVKGPASSLYGSEAVGGLINVITRSSLKAPRLSVDVMGTNWQEMNIDAGLKLK
                     AGKHFSLLGVNYFNYGNPIDNNGDGFTDLTLQNRISVFNKWDFMRKENRQANLAARYV
                     YEDRWGGQSNWTPYFRGGDSIYAESIYTSRFELIGNYQLALKKEKMFVNFSLSDHQQN
                     SVYGTTVFNARQRIGFVQQRWEKKIGKHELLSGAALRYTYYDDNTVATQSNDSVAAQN
                     KPQITPLPGIFIQDEFAIHQKHLLLGGLRYDHHFTHGPIFSPRISHKWKISDQLMLRT
                     GIGNGFRVVNVFTEDHAALNGSREVIISETLRPERSWNIYSNLLKKLYHRKWLATIEF
                     SVFATHFSNKIIPDYESNDDQIIYSNLNGYAQSKGAAINLEFNYSELIRINVGATFNT
                     IERIESGQKTRPLLTERFSGIFAISWKSSNGKWWADYTGNLIGPMLLPLQENDFRAPE
                     SPWFSIQNLQVSRKMGMAWNLYLGVKNLLNFTPPAWSIMRAHDPFDKNADNPQSNPFA
                     YRFDTTYVYSSFQGIRFFIGLRYSLP"
BASE COUNT          633 a          550 c          545 g          705 t
ORIGIN      
        1 atttccgctg ttagaaaaga cggataacga tacaccagtt tataattctt atcgaggata
       61 acgatagtcg ggtaggagag cgtattcgat tccattccca cttccaatgc aagcgcattc
      121 gcaccttttc cgtcgcgcac aattttaaag ctgtattctt ttccgttaaa acgcaaggtg
      181 tcttttgttt caccgtcgag ttttatcgca taaaacgatt gactcatttc tcggatgaca
      241 tacttgtttg tgaaggtgcg tttatccatg agtttacacg gaccgcacca caccgcgtag
      301 atatcgataa aaatgggttt gggattcacc ctcatcgaat cttcgagttg ctgaaaggtg
      361 agccaggtga tggtagtttt ttgttttttg gtggtatcct tttgcgccaa cacatcgctg
      421 caattgcctg ccgcaagaaa aagaagcagt aacaggttgc gatatgtaac gattgcgttc
      481 atggtaacga atacctcaag ccaatgaaaa agcgaatgcc ttgaaaggaa gagtaaacat
      541 acgtcgtatc aaagcgatat gcaaaggggt tgctctgagg gttatcagca tttttatcaa
      601 aagggtcgtg tgcgcgcata attgaccaag ccggcggggt gaaattgagc aggttcttta
      661 cacccaggta cagattccat gccattccca ttttgcggct cacctggagg ttctgaatgc
      721 tgaaccaagg ggattccggc gcccggaaat cgttctcctg caggggtaac agcatcggac
      781 caatcaggtt ccctgtataa tcggcccacc attttccatt gctgcttttc cagctgatgg
      841 caaaaatccc tgaaaatcgt tcggtcagca aagggcgtgt cttttgtcct gattctatac
      901 gttcaatcgt gttgaaggtt gctcccacat tgatgcggat cagttcgctg taattgaatt
      961 ccagattgat ggccgctcct ttcgattggg cataaccatt cagattcgaa taaatgatct
     1021 gatcgtcgtt gctttcgtaa tcgggaatga tcttattgga aaaatgcgtg gcaaacacac
     1081 tgaattcaat ggtggctaac cattttcggt gatagagttt cttgagcaga ttggagtaaa
     1141 tattccatga tcgttccgga cgaagggttt cgctaatgat gacttccctt gatccgttca
     1201 gtgcggcatg atcttcagta aacacattca ccactctgaa accgttccca attccggtgc
     1261 gcaacatcaa ctgatcgctg attttccatt tgtgactgat ccgcggtgaa aagatgggtc
     1321 catgcgtgaa atgatgatcg taacggagtc cgccgagcag taaatgtttt tggtgtatcg
     1381 caaattcatc ctgaataaaa attccgggca gtggtgtgat ctgtggtttg ttttgcgccg
     1441 cgactgaatc gttactttga gttgctaccg tattgtcgtc gtaatacgtg taccgcaatg
     1501 ctgctccaga tagtaattcg tgttttccga ttttcttttc ccagcgttgc tgaacaaatc
     1561 caattcgttg ccgcgcattg aaaacggtag taccgtaaac tgaattctgc tgatgatcgc
     1621 tcaatgaaaa attgacaaac atcttttctt ttttcagtgc taactgataa ttaccgatca
     1681 actcgaaacg cgaagtgtaa atggattccg cataaatact gtcgcctcct ctgaaataag
     1741 gtgtccagtt actttgtccg ccccatcgat cttcgtacac ataacgagcc gcgaggttgg
     1801 cctgacgatt ttccttgcgc atgaaatccc atttgttgaa aacggaaatg cggttctgca
     1861 atgtgagatc cgtaaatcca tctccgttgt tatcgattgg atttccataa ttgaaatagt
     1921 tgacccccag caatgaaaaa tgttttcctg cttttagttt gagtcctgca tcaatattca
     1981 tttcctgcca gttcgttccc atcacatcca cactgagtcg aggcgccttg agcgatgagc
     2041 gggtaatgac attgatcaat ccacctacag cttccgatcc atagagcgaa gacgcaggtc
     2101 cttttacaat ttcaatacgt tcaaccaacg cggttggaat tccgtacaaa ccgtacacac
     2161 ttgcgagtcc acttacaatc ggcattccgt cgatgagcac catcgtgtac ggaccttcca
     2221 ttccgttgat atgaatgtcg ccggtattgc aaaccgaaca attgatttgc ggacgaacac
     2281 cgttaactgt ttgcagagat tcaaaaaggg aaggcgcagg attttttcgc aggaaagaag
     2341 gagaaatgac agtaacggga ataggagatt ccatcaatcg tacttctttc atggtgcccg
     2401 atactaccac ttcatccaat agtgtttccg tac
//
LOCUS       DAOMYG010000463         2429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_16543_length_2429_cov_9.012637, whole genome shotgun sequence.
ACCESSION   DAOMYG010000463 DAOMYG010000000
VERSION     DAOMYG010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16543_length_2429_cov_9.012637"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PK610_12560"
     CDS             <1..477
                     /locus_tag="PK610_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010182141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HRJ36817.1"
                     /translation="PIIALSQLSRSVETRGGDKRPMLSDLRESGAIEQDADIVCFIYR
                     PEYYGITEDEKGQSLLGVGEIIIAKHRNGSLETVPLRFIPQFAKFDNLETFHFEDNYS
                     ANNMQPNTDFEGHSGGGAINPPQSMIRPSKMDQLNDDDDIGFGPIKPGGGYEEDPF"
     gene            705..1886
                     /locus_tag="PK610_12565"
     CDS             705..1886
                     /locus_tag="PK610_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HRJ36818.1"
                     /translation="MDNVQKNHLKSYGIAYWVLLGNVELEWLLNYRGGSFLIPFYKTI
                     QEECVIRGVSYEVITDARAQSIRLEIGDPEVNMEVVKMEKAPKIAVYAPKGHQPWDDA
                     VTMVMTYAEIPYDQIYDSAVVTGALVKYDWLHLHHEDFTGQYGKFYNSYRNAAWYQQQ
                     VRQTEAMAKFLGFAKVSELKLGVALRIKEYVAGGGFLFTMCSGTDSYDIALSAEGVDI
                     CGPMYDGDPLDPKAQSKIDFNRTFAFENYHLTMDPLVYEFSDIDGSQLHNLPENQDVF
                     TLFDFSAKWDIVPTMLCQNHTQVIKGFMGQTTDFVKKYIKSSVTVMGESKQFGTARYI
                     HGTFGKGTWTFYGGHDPEDFRHYVGDPPTDLNLHPNSPGYRLILNNILFPAAKKKKQK
                     T"
     gene            2030..>2429
                     /locus_tag="PK610_12570"
     CDS             2030..>2429
                     /locus_tag="PK610_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36819.1"
                     /translation="MTDAEEYIAVREEITVEGFFKATFYVNDVLEIFFDHSVEIVETI
                     HLQKIQEVAGKLGGGKKLNLLFHMHEFLHTSPEGSKFATSEEGVRFSNMIAVIVDNLP
                     KRILMNFFVTFHKPKVPTRGVKSKKDAFDWF"
BASE COUNT          717 a          469 c          538 g          705 t
ORIGIN      
        1 cctatcattg cattgtcgca gctgagtcgt tctgtcgaaa cccgcggtgg agataaacgt
       61 cctatgcttt ccgatcttcg tgaatcgggt gcgattgagc aggatgcgga tatcgtttgt
      121 ttcatctatc gtccggaata ttacggaatc accgaagatg aaaaaggtca gtccttactc
      181 ggtgtcggtg aaattattat tgccaaacac cgtaacggtt ctctcgaaac agttccgctg
      241 cgtttcattc cgcaattcgc caaattcgat aatctggaga ctttccattt tgaggataat
      301 tactctgcca ataacatgca acccaatact gattttgaag gccactccgg tggtggtgca
      361 atcaatccgc ctcaatctat gattcgtcct tcgaaaatgg atcagctcaa cgacgatgat
      421 gatattggct ttggtccgat caaaccaggt ggcggttacg aagaagatcc tttctgatga
      481 cttggaatac gattatggca ggattttcgt tatacacatt aaaggaggaa tcagctcaag
      541 gctaaacgcg agaaatgaaa atagtttcaa catatcatca tcagtcaacc ggatcaaatc
      601 tttcggttgt tcgccggtgc tcaccaatca ccatttactt ttcacttttc acttttttta
      661 ttctttcatt taattccgtt aaagcaaatt atctgcttct cccaatggat aatgtgcaga
      721 agaaccattt gaaatcgtac ggaattgcat attgggtgtt gctgggtaat gttgaactcg
      781 aatggttgct gaattaccgt ggaggaagtt ttttaattcc gttttacaaa accattcagg
      841 aagaatgcgt gataagaggg gtgagttatg aggtgatcac cgatgcaaga gctcaatcga
      901 tcagacttga aatcggtgat ccggaagtga acatggaagt ggtgaaaatg gaaaaagcac
      961 caaagattgc agtttacgca ccaaaaggac atcaaccctg ggatgatgct gtgaccatgg
     1021 taatgactta tgctgaaatt ccctatgatc agatctacga ttcggcagtg gtaaccggtg
     1081 cattggtaaa atacgattgg ttgcatttgc accacgaaga ttttaccggt cagtatggaa
     1141 agttttacaa ttcgtaccgc aacgcagcat ggtaccagca acaggtgcga caaacagaag
     1201 caatggcaaa atttctgggt tttgcgaagg tttctgaact caaattaggt gttgcacttc
     1261 gtattaagga atacgttgca ggtggcggat ttctatttac catgtgttcg ggaacagatt
     1321 cctacgatat tgcattgtct gcagaaggag tagatatctg cggtccgatg tacgacggtg
     1381 atccgcttga tcccaaggcg cagtcgaaaa ttgattttaa ccggacattc gcattcgaga
     1441 attaccatct cacaatggat ccgcttgtat atgaattctc ggatatcgac ggatcacaat
     1501 tgcacaacct ccccgagaat caggatgtat ttacgctttt tgatttttct gcaaaatggg
     1561 atattgttcc aaccatgtta tgccagaatc acacgcaggt tattaaaggc tttatgggac
     1621 aaacaaccga ttttgtaaag aagtatatca agagcagtgt tacggtgatg ggggaaagca
     1681 agcagtttgg aacggcaaga tatattcacg gtacattcgg aaaaggcacc tggacctttt
     1741 acggcggcca cgatcctgaa gatttccgtc actatgttgg cgatccacca acggatctta
     1801 atttgcatcc caattcaccg gggtatcggc ttattctcaa taatatccta tttccggccg
     1861 ctaagaagaa aaagcagaaa acctgatatg aagggaaagg ccgtgtcgtt tattctgtaa
     1921 acgattgtaa tacagaacga atattaaaaa tagaatgaaa tcacaatgat tatttgcgat
     1981 tatcctgtat tcgggttata ttcattttca agaactaaat tgcaaaaggt tgaccgacgc
     2041 agaggaatat attgcagtac gcgaagaaat cactgtagaa ggctttttta aggcaacgtt
     2101 ctatgtcaat gatgtgcttg aaattttctt cgatcactca gtagaaattg ttgagactat
     2161 tcatttgcaa aagatacagg aagtagcagg aaaactggga ggagggaaaa aactgaatct
     2221 tctttttcat atgcacgaat ttttgcatac ttcacctgag ggttctaaat tcgcaacttc
     2281 agaagaagga gtacgcttct ccaatatgat tgctgttatt gttgataatc ttcccaaaag
     2341 gattttaatg aattttttcg ttacttttca taaaccaaaa gtccctacgc gtggagtaaa
     2401 atccaagaag gatgcatttg attggtttt
//
LOCUS       DAOMYG010000464         2429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_16544_length_2429_cov_8.040438, whole genome shotgun sequence.
ACCESSION   DAOMYG010000464 DAOMYG010000000
VERSION     DAOMYG010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16544_length_2429_cov_8.040438"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PK610_12575"
     CDS             <1..458
                     /locus_tag="PK610_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HRJ36820.1"
                     /translation="ITGWNEYSLLPANSNIYFRGTFIGKSYLQTNTTKDTLELSLGAD
                     DGVIVQRKNVSEMQSKSATGNTKKKQEDFEITIRNTKSIAIEIEIEDQIPLSVQKEIT
                     VDVLEISGAEYNKENGKLIWKLSVPPGETKKLKLSYVVKYPKNYVIGNL"
     gene            470..2143
                     /locus_tag="PK610_12580"
     CDS             470..2143
                     /locus_tag="PK610_12580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HRJ36821.1"
                     /translation="MKTKILIPIFALLMQVTVTAQSKESETESKISAVTVFFSGAQIN
                     RKATVNLQPGTNIIRFSRLSYYIDPNSIQVEGSNKFTIVSVNHRHNFLNDADELPEVK
                     PLKAKIDKLTEELELLKIDMNANQEEREMILANKQFIGANKSVTVDDMMDMSELYQNK
                     YAKLLKDHAEFQKKEKELKKDLKRHRDQMKVVTAGKNLYTSDIIVNLSSPAKTETELS
                     FKYVTSNAGWTSAYDAKAIDVKNPLSLTYKAKVVQTTGEDWKNIKLTLSTGNPTRNNN
                     RPTLATWQLSGYDQEEAERRRKAEENRYQNKEASKKREKTAEAGAPSMAGAEVDMLEE
                     EETVSKDKSKSTASLTTVQQDAVSLEFSIAIPYTIASDGKDYTVEIARHELNASYRYY
                     ASPKYDNSAFLVSYISGWEKFNLMSGMASVYFGDNYIGQSYFNTSSTLDSLELSLGRD
                     KSILLERKKIKDYAKPSLSGSTRKVTLGIELIVKNTRSSAIKLDLEDQYPVTRNKEIE
                     VELIENGGATVKKETGTLTWSLDFNPGETKTIRFAYSVKYPKDKDISNF"
BASE COUNT          867 a          482 c          492 g          588 t
ORIGIN      
        1 gcattacagg atggaatgaa tacagtctcc ttcctgccaa cagtaatatt tatttcaggg
       61 gaacattcat tggtaaatcc tacctgcaaa cgaatactac aaaagataca ctggagcttt
      121 cgcttggagc agatgatggt gtaattgttc aacgcaagaa tgtttcggaa atgcaatcca
      181 aatcggctac gggaaacaca aaaaagaaac aggaagattt tgagatcact attcgaaata
      241 caaaatctat tgcaatcgaa attgaaattg aagatcagat tccactttcc gtacagaaag
      301 aaattacggt tgatgtactc gagatttcag gcgcagaata caacaaggaa aacggaaaac
      361 tcatttggaa actgagcgtc ccacccggtg aaacgaaaaa actcaaactg agttatgtcg
      421 tgaagtatcc gaagaattat gtaattggta acctttaatg ctaacggaca tgaaaacaaa
      481 aattctcatc ccgattttcg ctcttttgat gcaggtaact gttactgctc aatccaagga
      541 aagtgaaact gaatcaaaaa tatctgcggt aacggtattc ttttccggag ctcagatcaa
      601 cagaaaggct acagtcaatc ttcagccggg tacaaatatt atccgattca gccgattgtc
      661 gtattatatc gaccccaaca gcattcaggt agaaggaagc aataagttca ccatcgtatc
      721 ggtaaatcac cgtcacaatt tccttaacga cgccgatgaa ttgccggagg taaagccatt
      781 aaaggcaaaa attgacaagc ttacggaaga attggaattg ctcaaaatcg acatgaatgc
      841 caaccaggaa gaaagggaaa tgattctcgc caacaaacaa tttattggag ccaataaatc
      901 tgttacagtg gatgacatga tggacatgag cgaattgtat cagaacaaat atgcaaagct
      961 gttaaaggac catgctgaat tccagaagaa agaaaaggaa ctaaaaaaag atctcaagcg
     1021 acaccgcgac caaatgaaag tagttactgc aggcaaaaac ctgtacactt ctgatatcat
     1081 tgtaaatctt tcctctcctg caaaaactga gactgaactg tcattcaaat atgtaacctc
     1141 aaatgcaggg tggacatctg cctatgatgc aaaagcgatt gatgtaaaaa atcccctttc
     1201 gcttacctac aaagcaaaag tagttcagac aacaggagaa gactggaaga acatcaaact
     1261 cacactaagc accggaaatc caacaaggaa taataatcgt cctacactag ccacctggca
     1321 attgagtggt tacgatcagg aagaagccga gcgccggcgc aaagctgaag aaaaccgtta
     1381 ccagaataaa gaagcttcaa aaaaacgtga gaaaactgct gaagcaggag ctccttcaat
     1441 ggcaggtgct gaagtagata tgttagagga agaagaaaca gtgagcaagg ataaatccaa
     1501 aagcacagcc tcactgacta ccgttcaaca agatgcggta agtctggaat tttcgattgc
     1561 cattccctac actattgcat ctgatggaaa agattatacg gttgaaatcg cacgccacga
     1621 actgaatgcc agttaccgtt attatgcttc tccgaaatac gacaactctg catttttggt
     1681 aagctacatc agtggttggg aaaaattcaa tctgatgagc ggaatggcaa gtgtttattt
     1741 tggggataat tatatcggac aatcatattt caatacaagc tctacacttg attcgcttga
     1801 actttcattg ggcagagata aaagtatatt actggaacga aagaaaatta aagattacgc
     1861 caaacccagt ctttccggat caaccagaaa agttacgctg ggaattgaac tgattgtaaa
     1921 aaatacacgc agctcagcca ttaaactcga cctcgaagac cagtatccgg taacacggaa
     1981 taaggaaatt gaagtagaat taattgaaaa tggcggggcc acagtaaaaa aggaaaccgg
     2041 aacgcttacc tggtcacttg atttcaatcc gggtgaaacc aaaactattc gctttgcata
     2101 cagtgtaaaa tatccgaagg ataaagacat cagtaatttt taagcctcaa ttccggatag
     2161 taccaatggg aacagcagaa ataagtgcaa tgaaaaagtg gggaaataag tactcctctt
     2221 taaccggtgt atggaaaatg aattatcggt acaggaattt cataatcaca atatccccgg
     2281 aaaattctcg catttattcg cacttttcac ctttcacctt tcacccatga acaatgtggt
     2341 ataaagaatg gttcgattgt gagtactacc acattcttta ttgtcaccgc gatgataaag
     2401 aggccgaagc ttttttgcat cggcttgtt
//
LOCUS       DAOMYG010000465         2418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_16647_length_2418_cov_6.585696, whole genome shotgun sequence.
ACCESSION   DAOMYG010000465 DAOMYG010000000
VERSION     DAOMYG010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16647_length_2418_cov_6.585696"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..2097
                     /locus_tag="PK610_12585"
     CDS             67..2097
                     /locus_tag="PK610_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ36822.1"
                     /translation="MKRILTLFLFFLLSTLAMGQSKKLWLYYADNFYKGKDYFSALNY
                     YQKALDDTSIMELKVRPYEPDLMNLPMKSEIKQGERTVPLRDYVLHRIAMCHKQSFDY
                     PASVGSFTTSKATGSYPEDQYFLAYSYMQLKNYDTAVIEFEKFLQLANKSDSLTKCTE
                     RDMIGCYFAKDLANMKEEVKIRMLDTATINKGTANFAASFWGTADKIIFTSAREGGVL
                     IDPEKQQSEYLCDLYWSERKGEEAWSAPKNFGRPVNTAQHDGAGAISIDEFLYFTRWS
                     DTRRKEQTIFKARMMNGMFFEAYRMDSSVNIGGYRSVHPFVTIDGKLLYFSSDRPGGL
                     GGLDIWVCDLDEEGNPSNPRNLGPSVNTPYDEVTPFFHHVSSTLFFSSNGHNSTGGLD
                     IFKSYYNIDEEVFNSAVNIGMPFNSPQDDAYFTADRFLKHGYFSSDREECKGGHCYDI
                     YEFYNSPIAFKLSGFAYNADTDEILPNTLLTFNDVSGEAEPVFVTTDENGFYSIDLKQ
                     EQELFIKAKKSKFFADAATVSTFGLTESTDLEQDFLLRPIPTGEIEIPGIEYDYDKAT
                     LRPKSKEILDQLYDFLMLNNDLVVEIKSHTDCRGNDAYNMRLSKDRAKSCVDYLISKG
                     IPKERLVPQGYGESEPLFTCDEIETHKKSDPAKFEEMHQRNRRTAFRVMKQE"
     gene            2120..>2418
                     /locus_tag="PK610_12590"
     CDS             2120..>2418
                     /locus_tag="PK610_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36823.1"
                     /translation="MKTYLSTLCIVALLFSSCDQTTGTKENGSLIANKTISYINAPGS
                     GKQFAERSPISLELTLRSVPEDLDSVIFYINGEIVYSSIERSEKYSFSYSDTLNQ"
BASE COUNT          758 a          501 c          484 g          675 t
ORIGIN      
        1 aacctatatc agcaaaaaac gcagcagtaa agaatttaaa atttgtcccc gcatttgatc
       61 tcaaacatga aacgcatact tacactattc ctcttcttcc ttttatcgac actcgcaatg
      121 ggtcaatcga aaaaattgtg gttgtactat gccgacaatt tctacaaggg aaaagattat
      181 ttctcggccc tgaattatta ccagaaggcg cttgatgata ccagtatcat ggaactaaag
      241 gttcgtccct atgaacccga cctgatgaat cttcctatga aatcggaaat caaacaaggt
      301 gagcggacag ttcctcttag ggattatgta ttgcaccgca ttgccatgtg ccataaacaa
      361 tctttcgatt atcctgcctc tgttggatca ttcaccacaa gtaaagcaac cggttcctat
      421 cccgaagacc aatattttct tgcttattca tatatgcagt taaagaacta tgacactgct
      481 gttattgaat tcgaaaaatt tcttcagctc gccaacaaaa gtgattcact tacaaagtgt
      541 acggaacgtg atatgatcgg ttgctatttt gcaaaggatc ttgctaacat gaaagaagaa
      601 gttaagatcc gaatgcttga tacggcaacg atcaataaag gaactgccaa ttttgctgca
      661 tctttttggg gaactgcaga caaaatcatt ttcaccagtg cacgcgaagg cggggtattg
      721 attgatcctg agaaacaaca atcggaatac ctctgtgatt tatattggtc ggaaagaaaa
      781 ggagaagaag catggtcagc accaaaaaac ttcggccgac ctgtaaatac cgcccaacat
      841 gacggcgcag gtgcaataag tatagatgag tttttgtatt tcactcgctg gtcggacaca
      901 cgcagaaaag aacaaacgat ctttaaagcg cggatgatga acggaatgtt ttttgaagca
      961 taccgcatgg acagcagtgt aaatattgga ggatacagaa gcgttcatcc ttttgtaacc
     1021 attgatggta aactattgta tttctccagc gacagacccg gtggacttgg tggtcttgat
     1081 atctgggtgt gtgatttgga cgaagaagga aatccgagca atccacgcaa tcttggcccc
     1141 tccgttaaca ctccttatga tgaagtaaca ccttttttcc accatgtatc ttccaccctg
     1201 ttttttagtt cgaacggaca caacagtaca ggtggattgg atatttttaa aagctattac
     1261 aacatcgacg aagaagtatt taattcagct gttaatattg gaatgccctt taattcacct
     1321 caggatgatg cctattttac tgcagaccgt tttttaaaac acggatattt ctcatctgac
     1381 agggaagaat gcaagggtgg tcactgttat gatatttacg aattctataa ttctccgatt
     1441 gcatttaaat tatcgggatt tgcatataat gctgatactg atgaaatttt gccaaacaca
     1501 ttgctaacct ttaacgatgt aagtggtgaa gccgagcctg tgtttgtaac aacagatgaa
     1561 aacggattct attccattga cttaaaacaa gaacaggaac ttttcatcaa ggccaaaaaa
     1621 tcgaaattct ttgcagatgc agcaacagta agcacatttg gcttaacaga aagtaccgac
     1681 ctggaacagg actttttgct tcgccctatt ccaacaggag aaattgaaat tccgggtatt
     1741 gagtacgatt acgacaaagc cacacttcgt ccgaaatcaa aggagattct tgatcagctt
     1801 tacgacttcc tgatgctcaa caatgatctt gttgttgaaa tcaaatcgca cacagattgc
     1861 cgtggaaacg atgcctacaa tatgcgatta tcaaaagacc gtgcaaaatc ttgtgtcgac
     1921 tacctgatct cgaaaggaat tcccaaagaa cgacttgtac ctcaagggta tggagagtcg
     1981 gaaccgctct ttacctgcga tgaaattgaa acgcataaga aaagtgatcc tgcaaaattt
     2041 gaagagatgc accagcgcaa tagacgaact gcattccgcg ttatgaagca ggaataagca
     2101 ggaattctac ttttgcagca tgaaaacgta tctatcaact ctttgtatcg ttgcgctttt
     2161 attttcctcc tgcgatcaaa caacgggaac aaaagaaaat gggagtctca ttgcgaataa
     2221 gaccatcagt tatatcaacg ctccgggaag cgggaaacaa tttgctgagc gatctccaat
     2281 cagtcttgag ctaaccttac gttctgttcc ggaagatctt gattcggtca ttttctatat
     2341 caatggggaa attgtttact ccagcattga acggtcagaa aaatattcgt tttcctattc
     2401 cgacactctc aatcaaat
//
LOCUS       DAOMYG010000466         2410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_16730_length_2410_cov_6.521868, whole genome shotgun sequence.
ACCESSION   DAOMYG010000466 DAOMYG010000000
VERSION     DAOMYG010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16730_length_2410_cov_6.521868"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PK610_12595"
     CDS             <1..528
                     /locus_tag="PK610_12595"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ36824.1"
                     /translation="PSLFHIGAMDWTPNSEGIEWFLKEVWPIVRKSYPNLKFHLAGRK
                     MPDDLMNNAPENVIVYGEVKSATAFMNSKSIMVVPLLTAGGMRVKIIEAMALGKAVIS
                     TRIGAEGIDCRKGENILIANSPEEFSQAISQLIENQQLFTSIGINARKLVEQHYDNRL
                     LTRDLLNFYHSLRPA"
     gene            525..1685
                     /locus_tag="PK610_12600"
     CDS             525..1685
                     /locus_tag="PK610_12600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ36825.1"
                     /translation="MRLFIILSRVPWPLEKGDKLRAYYQIRELSRYHDVSLFCLSDTA
                     IDPKAKTELLRYCKEVHIERIRRPGILFRLAKAVFSKLPFQVHYFYKPSLKSKVHSII
                     REFNAEHIYCQLIRCSEYVKNIHHIPKTLDYMDAFSKGVERRIASAPFGIRSIFKTEA
                     RRLVEYENLIFDYFENKTIITEQDKRFIYHPDQQHIHVIPNGVDVTHFSPMERKKKYE
                     IVFNGNMNYPPNIDCVEYIAKEILPLLPPTTRFLISGASPAGQVLELRENSQITVSGW
                     VDDQRESYASGKVFLAPFRIGTGLQNKLLEAMSMGIPCVTSTLANNALGAEPGRQILI
                     GDSPTEIADLVAKLLNDKDRAAAIAEEGKSFVHSRYNWESSCLILNNLITGK"
     gene            1769..>2410
                     /gene="ribB"
                     /locus_tag="PK610_12605"
     CDS             1769..>2410
                     /gene="ribB"
                     /locus_tag="PK610_12605"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HRJ36826.1"
                     /translation="MNYQLNTIEEAIEDIRSGKVVIVVDDEDRENEGDFLTAARNATP
                     EVINFMATHGRGLICAPLVETRCDELGLALMVQANSAAYETPFTVSVDLIGHGTTTGI
                     SASDRAKTIQALIDPNTKPEELGKPGHIFPLRAKKEGVLRRAGHTEAAVDLSRMAGFE
                     PAGVIVEIMNEDGSMARLPELVKIAERFQLKIISIEDLIAYRLRTESMIERQIV"
BASE COUNT          720 a          482 c          525 g          683 t
ORIGIN      
        1 ccaagtttgt tccatattgg tgcaatggac tggactccaa attctgaggg aatagaatgg
       61 tttctaaaag aggtttggcc tattgtcaga aaatcatatc ccaatctgaa attccacctc
      121 gcaggtcgca aaatgccgga tgatctgatg aacaatgcac ctgagaatgt gatagtgtat
      181 ggtgaagtaa aaagtgcaac cgcattcatg aacagcaagt ccattatggt tgtccccttg
      241 ttgactgccg gaggaatgcg agtaaaaatc attgaagcaa tggccttggg aaaagctgtg
      301 atttcaacac gaattggcgc agaaggaatt gattgtagaa aaggagaaaa cattttaatt
      361 gcaaattcgc cggaagaatt ttcgcaggcc atttcgcagc ttatcgaaaa ccagcaactt
      421 tttacatcca ttggcattaa tgctcgtaaa ctggttgaac agcattatga taatcgtttg
      481 ttaacacgtg atcttttgaa tttttatcat agtttacgtc ctgcatgaga ttgttcatca
      541 ttctttccag agtaccctgg cctttggaaa agggcgataa actacgggca tattatcaga
      601 ttcgcgaact atcgagatat catgatgttt cgctgttttg tttgagtgat acagccatag
      661 atcccaaggc aaaaacagag ttgcttcgct attgtaaaga agttcatatt gaacgcatca
      721 gacgacccgg aatattgttc cgactcgcaa aagctgtgtt ttccaaactt ccgtttcagg
      781 tccattattt ttacaaacct tctttaaaaa gtaaggtgca ttcaattatc cgtgaattca
      841 atgcagaaca catttactgc caacttatca gatgcagtga gtatgtaaaa aatattcatc
      901 atatcccgaa aacgcttgac tacatggacg ctttttccaa aggagttgaa cgaagaattg
      961 cttctgctcc atttggaata cgttcaatat ttaaaactga agcacggaga ctggtcgaat
     1021 atgaaaatct gatttttgat tatttcgaaa acaaaaccat tattaccgaa caggataaac
     1081 ggtttattta tcaccccgat cagcaacaca ttcatgttat tcccaatggt gtagatgtaa
     1141 ctcatttttc gccaatggag cgaaagaaaa agtatgaaat tgtgtttaat gggaatatga
     1201 attatcctcc aaacatcgat tgcgtggagt acatcgcaaa ggaaattctg ccgcttctac
     1261 cccccactac ccgattcctc atttcgggtg catctcctgc agggcaagtt ttggaacttc
     1321 gggaaaattc ccaaataaca gttagtggtt gggtcgatga tcaaagggaa tcttatgcat
     1381 ccggaaaagt ttttttggca ccattcagga ttggtaccgg attgcaaaac aagcttcttg
     1441 aagcaatgtc catgggtatt ccctgtgtca cttcaacact cgccaacaat gcactgggag
     1501 ccgagccggg cagacaaatt ttgattgggg attcgccgac agaaatagca gatttggtag
     1561 ctaaacttct aaacgataag gaccgggctg ctgcaattgc tgaggaaggc aagtcatttg
     1621 tgcattcgag gtacaattgg gaatcttcct gcctgatttt gaacaatctt attactggga
     1681 aataagcaaa taactacaaa ctattcccca gttctttccc cggaaaaggt atataaatcc
     1741 ttacctttgc agtctaatta ttcagtgtat gaattaccag ttgaacacga tagaggaagc
     1801 gattgaagat attcggagcg ggaaggtcgt gattgttgtt gatgatgagg atcgggaaaa
     1861 tgaaggtgat tttcttactg cagccagaaa tgctactccg gaagtcatca attttatggc
     1921 tacccatggt agaggcttga tttgtgctcc attggtggaa acacgttgtg atgaattggg
     1981 acttgcctta atggttcaag ccaacagtgc tgcatacgaa accccattta cggtatctgt
     2041 agatttgatt ggtcacggta ctaccactgg catttctgca tcggatcgcg ccaagaccat
     2101 acaagcactt attgatccga acaccaaacc ggaagaatta ggaaaaccgg gacatatttt
     2161 ccctctacgc gcaaagaaag aaggtgtttt gcgcagagca ggtcatactg aagcagctgt
     2221 tgatctttcg cgcatggcag gattcgagcc ggccggtgtt attgttgaaa tcatgaacga
     2281 agacggttcc atggcacgcc ttcctgaatt ggtaaaaatt gcagaacgtt ttcaattgaa
     2341 aataatctct attgaagacc ttattgccta ccgtctccga accgaatcga tgatcgaacg
     2401 ccagattgtt
//
LOCUS       DAOMYG010000467         2396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_16889_length_2396_cov_8.422469, whole genome shotgun sequence.
ACCESSION   DAOMYG010000467 DAOMYG010000000
VERSION     DAOMYG010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16889_length_2396_cov_8.422469"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="PK610_12610"
     CDS             complement(<1..1431)
                     /locus_tag="PK610_12610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ36827.1"
                     /translation="MIKHLLCFSSALIFLVAPVSAQTTLFSENFNSGGPGGFTLNTSD
                     QSSIAAGVYNSWVVNSAYAGGSGSFVCFGFPLPFSVPATPNQPGGITGSPNSGYMHIN
                     SVAAASSGIHCASFMAADGLCSFSENYFTRMSSDISTIGQSTVTLNFWWLCAGGAQSY
                     GEVYYSTNGGSSWTLVTTPISQYFGQSAWTQQTISIPAFANQSTLRFGFRFVNATTSS
                     AADPAFALDDIVITGSGTACTPTFSSTVHTACSSYVWNGNTYTASGNYTFLTTNAGGC
                     DSTATLNLTINNPSTSTTAQTACVSYLWNGNTYTTSGTYTYVTTNSQGCDSTATLNLT
                     INNANTNVSQVGNTLTANATGASFQWVDCNNSNQPVPGATAQSFSPITNGSYAVQVTQ
                     NGCTDLSACFNVTTIGLDKTSFARSIDYFPNPTTGELNIRFNAAAENISIRILNVVGQ
                     VVSTSTVNGSNLTTVFINGEPGLYFIE"
     gene            complement(1540..1986)
                     /locus_tag="PK610_12615"
     CDS             complement(1540..1986)
                     /locus_tag="PK610_12615"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRJ36828.1"
                     /translation="MDPFVYMLDREAVGLVVNQLFYALDNREWSSLQECFSPRVEFDI
                     TSLIGGEKHHLRPEQIATLWSNSLSEVQHLQHQITNLTVFVQNERATAHFYCSETHFK
                     QLPSGNNTRSLNGSYDIHLTKESGVWKIHLFRYNHKFISGNAALKS"
     gene            2171..>2396
                     /locus_tag="PK610_12620"
     CDS             2171..>2396
                     /locus_tag="PK610_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36829.1"
                     /translation="MKNGSKKIILLTLLIFCAGLVQAQMPYSVFKQRYSPHNNTILDS
                     KNDGVKTANGLSSKELAGYLDTYVVQEEKQV"
BASE COUNT          756 a          484 c          494 g          662 t
ORIGIN      
        1 ctcaataaaa tacaaaccgg gttcaccgtt aatgaaaacc gtggtaagat tgcttccatt
       61 aacagttgaa gtggacacaa cctgtcccac aacatttaga atgcgaatgg aaatattctc
      121 agcggcggcg ttaaatcgaa tatttaattc gccggtagtt ggattgggga aatagtcaat
      181 actccgggca aaggaagttt tatccaatcc aattgttgtc acattaaaac aagctgaaag
      241 atcggtacaa ccattctgcg taacctgaac tgcatagctt ccattggtaa tcggagaaaa
      301 actttgagca gttgcacccg gtacaggctg attgctgtta ttgcaatcga cccattgaaa
      361 acttgctccg gttgcgtttg cagttagtgt gtttccaacc tgagatacgt tcgtattcgc
      421 attgttaata gttagattta atgttgctgt agagtcgcat ccctgagaat tggttgtaac
      481 ataggtatac gtaccggaag ttgtataggt atttccattc cataggtaac tgacacatgc
      541 tgtttgcgcg gttgtacttg tgctgggatt attaatcgtt aaattcagcg tagctgtaga
      601 atcgcagccg cccgcattgg tcgttaaaaa ggtatagttt ccgcttgcag tataggtgtt
      661 tccattccaa acatagctgg agcaggccgt atgaaccgta gaactgaatg ttggcgtaca
      721 tgctgttccg cttcctgtaa taacaatatc atccaatgca aaagcaggat ctgctgcaga
      781 agatgttgtt gcgtttacaa agcgaaatcc aaaacgtaaa gtagattgat tggcaaatgc
      841 aggtattgaa atagtttgtt gagtccaggc tgattgtccg aaatactgag aaatcggagt
      901 cgttaccaat gtccatgatg aaccaccatt cgtgctgtag taaacctcac cataactttg
      961 tgcaccacca gcacaaagcc accagaaatt tagtgttacc gtagattgtc caatcgttga
     1021 aatatcggat gacattcttg taaaataatt ttcactaaat gaacatagtc catcagctgc
     1081 cataaatgat gcacaatgaa ttccagatga agctgctgct acactgttaa tatgcatata
     1141 tccgctattg ggacttcctg taataccacc tggttggttg ggtgtagcag gaactgaaaa
     1201 tggtaaagga aatccaaaac aaacaaaaga accgcttcca cctgcatagg cagaatttac
     1261 tacccatgaa ttatacacac ctgcagcaat tgatgattga tcggatgtat taagcgtaaa
     1321 tccaccggga cctccggaat taaaattctc actaaacagg gtggtctgcg cagaaaccgg
     1381 tgctacaagg aaaataagcg ctgaactgaa acaaagtaaa tgtttaatca tctctattgg
     1441 ttttggttgg taaatggact tgctaccaaa tataaccaaa caattctgca attccaatat
     1501 cacggaatga gatgaaaacc actctattag ttaaagctac taactcttaa gggcagcgtt
     1561 ccctgatatg aatttgtgat tgtacctaaa caagtgaatt ttccacactc cactttcttt
     1621 ggtgagatga atatcatagc taccgttcaa tgaacgtgta ttgtttccgg aaggcaattg
     1681 cttgaagtga gtctccgaac aataaaaatg tgcggttgct ctttcgttct gaacaaacac
     1741 tgtaaggttg gttatctggt gctgcaaatg ctgtacttca gataatgaat tactccataa
     1801 agtggcaatc tgttctggac gcaaatggtg tttctctcca ccaatcaatg acgtaatatc
     1861 aaattcaacc cgcggagaaa aacattcctg tagcgacgac cattctctat tgtcgagtgc
     1921 atagaacaat tgattaacca caagtccaac agcctcgcgg tccagcatat atacaaaggg
     1981 atccataatt ctaaactaat gattttttta aaatggagtt aaatggtttg ccggataggt
     2041 gggcaaatcg aatccaccct aaagcttggc ataacctgga agacatgaag atatctgctt
     2101 aaaatacggt aatcaacgta tgttggaggc cagcaggact aaccattttt gttaaaccaa
     2161 acaaaccctc atgaaaaacg gatccaaaaa aattatcctg ctgacattac tgatattttg
     2221 cgcaggattg gttcaggcac aaatgcctta cagcgtcttt aaacaacgct attcacccca
     2281 caacaacacg attcttgact caaagaatga cggggtaaaa acagcaaatg gcctaagttc
     2341 aaaggaactg gcaggatatc tcgatactta tgttgtgcag gaagaaaaac aggttt
//
LOCUS       DAOMYG010000468         2394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_16907_length_2394_cov_10.981189, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000468 DAOMYG010000000
VERSION     DAOMYG010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16907_length_2394_cov_10.981189"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1769
                     /locus_tag="PK610_12625"
     CDS             <1..1769
                     /locus_tag="PK610_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36830.1"
                     /translation="IPKMNPTFEMEFYARINRALLYEGGDSRGIRAELMKLLRDEKNK
                     EYFDQIYYALAELELKDDKRDQGVGYLKKSIAVSVSNERQKGRSYLRLGDLHFNDRDY
                     VPAKNFFDSSLAFLPKTFPDYEKIKKKSESLSELVTHLNTITLQDSLLNLASLSPKKR
                     QEAIDKIVEKEKEEEEAKRRAEEAKIQQGTTGPTVAAESGGWYIYNQQAKTQGFNEFK
                     KLWGARKLEDDWRRSNKTSVSEEEISNTGDSALSATDAKYEKRREQLLSSIPNGPEQI
                     SESKKKLQEAYYKSGVIFKDRFQQKDLAIKQFKSLIDRFDYGEYVLPANYQLYLIYNP
                     SPQSEAYKNTILNDYADSEYARLIRNPNAMKEEEELEKKQEVVYLQAYKLYREKRYSE
                     SLEACNQIIQSEPQNSYLSRYYFLRAMNYGEMKQMDKLESSLSECAEKYAAEDTGKEA
                     QSILDYLRNKRSKENPIQGFVYEAEAEHYFMMIFTSDLGSINDGKAAFSDFNSQYFSN
                     ADLKVSNGFLNMETQTLVVKQFPNRKKAMEYYQAYKNENDRLKKYKGATCFMITGKNN
                     AAFMLERDVEKYMSFFTENYLK"
     gene            complement(1770..2267)
                     /locus_tag="PK610_12630"
     CDS             complement(1770..2267)
                     /locus_tag="PK610_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36831.1"
                     /translation="MRKLIIVLFISLPFSVSAKLNDDWKWLVIQDGFMFPEETFANTL
                     VYDGGAWSLYYYLEQTTIVPPQQRRFLNGQQMVLFKMNFDSPGKLTYIEPVERYYWYS
                     VDGLAKPFIEDVMKILANAPGWNTAFSGEVYLPLRFRISGGRMFVDYNSFVTMGVGKV
                     KIGRR"
BASE COUNT          814 a          510 c          477 g          593 t
ORIGIN      
        1 aaattccaaa aatgaaccca acgttcgaaa tggagtttta tgctcgtatc aaccgcgctt
       61 tgctctatga gggtggcgat agtcgcggaa ttcgtgctga gctgatgaag ttgttacgtg
      121 atgaaaagaa caaggaatat tttgatcaaa tttattatgc acttgcagag ctggaattaa
      181 aagatgacaa acgcgatcag ggagtaggtt atctgaaaaa atcgattgcc gttagcgtaa
      241 gcaacgaacg acaaaaaggg cgctcctacc tgcgtcttgg cgacctccat ttcaacgacc
      301 gcgattatgt tcctgcaaaa aacttcttcg atagcagttt agcattcttg cccaaaacct
      361 ttccggatta tgaaaaaata aaaaagaaaa gcgaaagctt atctgaactt gttacccacc
      421 tcaacaccat aacgctacag gatagtctcc tgaatcttgc ctctctttct cccaaaaaaa
      481 gacaagaagc aattgataag atcgtagaaa aagagaagga agaagaagag gccaagcgcc
      541 gtgcagaaga agcaaaaatt cagcagggaa caacggggcc tacggtagca gcagaaagcg
      601 ggggctggta catttacaac caacaggcaa aaacacaggg gttcaatgaa tttaaaaaat
      661 tgtggggcgc ccgtaaactg gaagacgatt ggagacgatc caataaaaca tccgtcagtg
      721 aagaagagat tagtaatacc ggggatagcg cattatctgc aacggatgcc aagtatgaaa
      781 aaagaagaga acaactcctg agcagtatcc cgaatggccc tgaacaaatc tctgaatcga
      841 agaaaaaact acaagaggcc tattacaaat cgggggtaat attcaaagat cgctttcaac
      901 aaaaagattt ggccattaaa cagtttaaat cgctgatcga tagatttgac tatggagaat
      961 atgttttacc tgcaaactat cagctctatc tgatttataa tcccagtccg caaagcgagg
     1021 cctataaaaa cactattctc aacgattatg ccgattcgga atatgcccgt ttgatccgaa
     1081 atcccaatgc gatgaaggaa gaggaagagc tagagaaaaa gcaggaagtg gtgtacctcc
     1141 aggcctacaa actttatcgc gaaaaaagat attctgaatc gttggaggcc tgtaaccaga
     1201 ttattcagtc ggaaccgcaa aacagttatc tctcccgcta ttacttcctg cgcgccatga
     1261 attatggtga aatgaaacaa atggataaac tggagtcctc gctaagtgaa tgcgctgaaa
     1321 aatacgctgc tgaagacacc ggtaaagaag cccagtctat actcgactat ttgcgcaaca
     1381 aacgctctaa ggaaaacccg attcagggct ttgtttatga ggccgaggca gaacattatt
     1441 ttatgatgat tttcacctcc gatctgggtt cgattaacga tggaaaggct gcgttttccg
     1501 actttaattc gcaatatttt tccaatgctg atttaaaagt gagcaacgga ttcttaaaca
     1561 tggaaacaca aaccctcgtt gttaagcagt ttcctaaccg aaaaaaggca atggagtatt
     1621 accaggcata taagaacgaa aacgatcgtc tgaaaaaata caaaggcgct acctgtttta
     1681 tgattacagg taaaaacaac gcagcattta tgctcgaacg tgatgtggaa aaatacatga
     1741 gcttttttac agagaattac cttaagtaat taacgtcttc cgatttttac cttccccacg
     1801 cccattgtaa cgaacgaatt gtaatccaca aacattcgtc cccccgaaat ccgaaagcgc
     1861 aaaggcaaat acacttctcc tgaaaaagca gtattccaac ccggcgcatt ggcgagaatc
     1921 ttcataacat cctcaataaa tggtttggcc agaccatcaa cagaatacca gtaatacctt
     1981 tccaccggct caatgtaagt gagttttccg gggctgtcga aattcatttt aaacaaaacc
     2041 atctgttgtc catttaaaaa tctcctctgc tgcgggggaa caattgtagt ctgctccaaa
     2101 taataataca gactccaggc tcctccatcg tataccaatg tgtttgcgaa agtctcctca
     2161 gggaacataa atccatcctg aataaccaac cacttccaat catcattcag ttttgcagac
     2221 accgaaaatg gtaatgaaat gaatagcacg attatcaact tacgcataaa gaggagcata
     2281 caacagcaat ttacacaatt aatacgctgt ttcagatttc agcttgttta aggtcttaaa
     2341 aattctattt ttgtatgcca tcgttttcac tatgtttaaa tcttcaaata cacc
//
LOCUS       DAOMYG010000469         2392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_16933_length_2392_cov_8.411211, whole genome shotgun sequence.
ACCESSION   DAOMYG010000469 DAOMYG010000000
VERSION     DAOMYG010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16933_length_2392_cov_8.411211"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PK610_12635"
     CDS             complement(<1..890)
                     /locus_tag="PK610_12635"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRJ36832.1"
                     /translation="MAISLKTRKRIFYSLLAGFALLVAGAFFANRWVKTKGYSNLWEF
                     ITTTSSNYGKSFNATYETLSITLDDDDFAKLEGQRQRALEQGIMTTEDDRYVNALLDH
                     NGTSIRAELRLKGHMTDHLQDKKWSFRIKTKKGDAFMGMKRFSLQHPGTRNYVHEWIY
                     HRMMEEEGIVALRYFYLRVKVNGDDWGVYALEEHFTQEMVEHNDRLPGPLIRYNPDLY
                     WVYRVNELHDVRIHEEYAQMQSSYFEAYESKSTLKDSAQRQLFINAVDRLEAFRTGKM
                     KTSEVFDIEKLARFHAIVDVV"
     gene            complement(1012..>2392)
                     /locus_tag="PK610_12640"
     CDS             complement(1012..>2392)
                     /locus_tag="PK610_12640"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRJ36833.1"
                     /translation="IAQMDENGKIYITSVSGLVVFDPLQVKLPENHPRLYFSGLIAQS
                     DTANRDQLITWLNEVPQFEWNQNNIQVEYYSDLLFNIAPPKYEYRLIGMDSVWKDNGS
                     SNRIRFTNLPHGKFTLEVRLTNAYGQQSDVLRWTFSIARPFWFSWWFYILVAIALSGL
                     VWVYIRYRERLLRERQLQLEQKVAERTSEVLQKAEEIRLQKDIISEKNKELTDSIHYA
                     QRIQQAMLPDDRAMMESMPEHFVLFKPKDIVSGDFYWHSHHPDCVLWAVVDCTGHGVP
                     GGFMSMLGSGLLNQIVNEEKVFRPDQILNLLRERVIIALKQSGEPGESRDGMDLGLVQ
                     YIPSKQQLHFAGAHNSLYIVRNGELLEYKADKQPIGIHGAEMKPFTLHAVSLQKGDHL
                     YMSSDGYSDQFGGEKGKKFKSSNFELLLKSISTESIARQHQILDETFTTWKGNFEQLD
                     DVCVIGVRL"
BASE COUNT          647 a          558 c          535 g          652 t
ORIGIN      
        1 accacatcta caatcgcatg aaagcgtgcg agtttttcta tgtcgaaaac ctctgaagtt
       61 ttcatttttc ctgtgcgaaa tgcttccagg cgatcaaccg cattaatgaa aagttgccgc
      121 tgcgctgaat ccttaagtgt acttttcgat tcgtaggctt caaaatacga agattgcatt
      181 tgcgcatatt cttcatgtat gcgaacatca tgcagttcat ttacgcggta aacccaatag
      241 agatccggat tgtaacggat aagcggaccg ggtaaacgat cgttgtgttc caccatctcc
      301 tgggtaaaat gctcttcgag tgcataaacg ccccaatcat ctccattcac ttttacacgc
      361 aaataaaaat agcgtaaagc cacaattcct tcttcctcca tcatgcggtg gtaaatccac
      421 tcgtgcacat aattgcgtgt tccgggatgc tgcagcgaaa aacgtttcat acccataaag
      481 gcatcaccct ttttggtctt gatcctgaac gaccattttt tatcctgtaa atgatcggtc
      541 atgtgcccct ttaagcgcaa ctccgcacga atggatgtgc cattgtgatc aagcaaagca
      601 ttgacatatc gatcgtcttc tgtggtcatg attccctgtt ccaaagcccg ctggcgctga
      661 ccttccaatt tggcaaaatc atcatcgtcg agggtaatcg ataaggtttc ataagtagca
      721 ttgaaactct ttccgtaatt ggaagaagtg gtagtgatga attcccagag attcgaatag
      781 ccttttgttt tcacccagcg gttagcaaaa aaagctcctg ctaccaaaag agcaaaacct
      841 gcaagtaaac tgtaaaatat ccgtttgcgt gttttcaggg agatggccat gggggcaaag
      901 atagtgattt caggaatggg cggaggggag agggaaccac taaggcacta agctcactaa
      961 gaaacactaa gcacttagtg cacctaggtg gccttagtgg ttaaaacctc cttacagtcg
     1021 gactcctata acgcacacat catctaactg ttcaaaattc cctttccagg tggtgaaggt
     1081 ttcgtcgagg atttggtgtt gacgggcgat ggattctgtg ctgatggatt tgagaagcag
     1141 ttcgaagttc gaggatttga attttttgcc tttctctcca ccgaattgat cggaatagcc
     1201 atcggaactc atatagagat gatcgccttt ttgcagggat acagcatgca atgtgaaagg
     1261 tttcatttct gcgccgtgaa tgccgatggg ttgtttgtcg gctttgtatt cgagcagttc
     1321 tccgttgcgc acaatgtaca gggagttatg tgctcctgca aaatgcagtt gctgtttcga
     1381 agggatatat tgtaccaaac ctaaatccat accgtctctg ctttcgccgg gttcgccgct
     1441 ttgtttcagg gcaatgatca cacgctcacg taaaagatta agaatctggt cgggacggaa
     1501 tactttctct tcattcacaa tctgattgag caatccggaa ccaagcatgc tcataaaccc
     1561 gccgggaact ccatgaccgg tgcaatcaac aactgcccac aacacacaat ccggatgatg
     1621 cgaatgccag taaaaatcac cgctgacaat atcttttggc ttaaacaaca caaaatgttc
     1681 cggcatactc tccatcatcg cgcgatcgtc gggcaacatg gcttgctgaa tacgttgtgc
     1741 gtagtgaatg gaatcggtga gctctttgtt cttttcggag atgatgtctt tctgcaaacg
     1801 gatctcttct gccttttgca atacttccga agtacgttct gccacctttt gttccagttg
     1861 taactgacgc tcgcgcaata ggcgttcgcg gtaacggatg tacacccaca caagaccgga
     1921 taatgcaatc gcaacaagaa tataaaacca ccacgaaaac cagaacggac gcgcaatgga
     1981 gaaggtccaa cgcaaaacat cgctttgctg accgtatgca ttggtcaagc gtacttcgag
     2041 tgtgaatttt ccgtgcggga gattggtaaa tcgaatacga ttggagcttc cgttatcttt
     2101 ccagacagaa tccattccga taagccggta ctcgtacttg ggcggcgcaa tattgaacag
     2161 cagatccgaa tagtattcca cctgaatatt gttctgattc cattcgaatt gaggaacctc
     2221 gttcaaccaa gtgatcaatt ggtcgcgatt ggcggtatcg gattgcgcaa taagtccgga
     2281 aaaatacaaa cgcggatgat tctcaggaag ctttacctgc agcggatcga agactacaag
     2341 cccgcttact gatgtgatat aaatcttacc gttttcatcc atctgcgcaa tg
//
LOCUS       DAOMYG010000470         2369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_17182_length_2369_cov_6.480553, whole genome shotgun sequence.
ACCESSION   DAOMYG010000470 DAOMYG010000000
VERSION     DAOMYG010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17182_length_2369_cov_6.480553"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1217
                     /locus_tag="PK610_12645"
     CDS             168..1217
                     /locus_tag="PK610_12645"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRJ36834.1"
                     /translation="MRPEYLSELLRDNKNVIIPGLGAFSTTGSTTQPYIFIEYLKFND
                     GLLASYISKKEGISVEEAGKKLEVFSAGMNSALETGKDISITGMGILKKNKEGKLEFT
                     CNINTQPLTPPVTPEKKTEVPVTPEQKIEKPEVKNNPLPVPPVIDKKTEAVPPKKETP
                     VVAKEIVEDKKKITTEKKEDKPKKEPKPPKAHKEKKPGSKKRLIWLIILLLLLGGGTT
                     TGILFKQEIIAWYNNTFGPKKEIVKPPEPKPAPEPEPEPEPEIIPEPEPEPTTITYTK
                     GKYYVVVGCFNEESNASGMVAKARAKGMPAAGLGRFGGMVHVAVYESDNLSDAAKKAM
                     ELRSDFPKAWVFQGR"
     gene            1252..2109
                     /locus_tag="PK610_12650"
     CDS             1252..2109
                     /locus_tag="PK610_12650"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ36835.1"
                     /translation="MRTYTICGVNEHFNFPWRYAIENKLFEQEGIHLSWTESKGGTGE
                     MTRMLRDGNTDLAVLLTEGIVQEILQNDSARIVQIHVRSPLHWGVHVHQESDIKSPSD
                     LRGRTFAISRNGSGSHLMAIVHARKNGWNEDELNFEIVNHLEGGLKSVQNKNSEVFLW
                     EKYTTAPFLAGHDLKYVGDVVTPWPGFVLAASINMCESHYQDLQLIGKTIASVCRSLR
                     NDHQIAEKIAKKHHLDVAQVELWLHELEWNEDQGISKGEQTTILHGLCNAGIIKETAI
                     PHPEKFFLE"
     gene            2106..>2369
                     /locus_tag="PK610_12655"
     CDS             2106..>2369
                     /locus_tag="PK610_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36836.1"
                     /translation="MIILRSLLLLSFIATCLNARSQEMIADSSWRIPDNLAETSGLSL
                     FGMNLLSHNDSSHPNLLIEMNRKGGFVRTLTVEQSKNVDWEELC"
BASE COUNT          797 a          484 c          493 g          595 t
ORIGIN      
        1 taatggagga cgaatttcca ttgcatctta aattcatttt tttcaaacag acgccacgaa
       61 tatgttggtg ccggttgtgt gaataatgca ttaaatcgtt gagaatacat ccaatgaaat
      121 tcgggatttt gacaattttt tattaaattc aggaaaattc atgagtgatg agaccagaat
      181 atttaagtga actactgcgc gacaacaaga atgtgatcat tcccggatta ggcgcttttt
      241 caacgaccgg cagtactact caaccttata tattcattga atacctcaag tttaatgatg
      301 gcttgcttgc atcctatatt tccaaaaaag aaggaatctc tgttgaagag gccggtaaaa
      361 aactcgaagt attttctgcc ggaatgaatt ctgctttgga aacagggaaa gacatttcaa
      421 ttaccggaat gggaattctc aaaaaaaaca aagaaggaaa attagaattc acttgtaata
      481 tcaataccca acctttaacc ccgccagtta ctcccgaaaa gaaaacagag gtcccagtta
      541 cacctgaaca aaagattgaa aaacctgaag taaaaaacaa tccccttccc gtacctccgg
      601 taattgataa aaaaacagaa gccgttcctc caaaaaaaga aacgccggtt gttgcaaagg
      661 aaatcgtaga agacaagaag aaaattacaa ccgaaaaaaa ggaagataaa ccaaaaaaag
      721 aacccaaacc accaaaagct cataaagaga aaaaaccagg aagcaaaaaa cgtttgattt
      781 ggctaatcat tcttttgcta ttacttggag gcggaacaac aacaggcatt ctgtttaaac
      841 aagaaattat tgcctggtac aacaacacgt tcggaccgaa aaaagaaatt gtaaaaccac
      901 cggaaccaaa acctgcgcct gaacccgagc cggagccgga gccggaaatt attccggagc
      961 ctgagcctga gcccactact attacttata caaaggggaa atattacgtg gttgtaggtt
     1021 gttttaatga agaatccaat gcaagtggta tggtagccaa agcacgcgca aaaggaatgc
     1081 ctgctgctgg tttgggtcgt tttggtggca tggtgcatgt tgcggtttac gagagcgaca
     1141 atctatcgga tgctgccaaa aaagcgatgg aattacgatc cgacttccca aaggcctggg
     1201 tttttcaagg cagatgatat catccactgt atctttgtca aaaaaaatca attgagaaca
     1261 tacaccattt gcggcgttaa cgaacatttt aattttccat ggaggtatgc catcgaaaat
     1321 aaactgttcg aacaggaagg cattcatctc agctggacag aaagcaaagg cggaaccggt
     1381 gaaatgacgc gaatgctccg cgatggcaat actgacctgg ccgtacttct taccgaagga
     1441 atcgtacagg aaattcttca gaatgattct gcacgaattg ttcaaattca tgttagaagt
     1501 ccattgcact ggggagttca tgtacatcag gaaagtgaca tcaaatcacc ctccgacctc
     1561 agaggacgca cattcgccat tagcagaaat ggatcaggat ctcatcttat ggccattgtg
     1621 catgcccgaa aaaatggatg gaatgaagat gaacttaatt ttgaaattgt caatcatctt
     1681 gagggtggat taaagtccgt tcaaaataaa aattcggaag tttttctttg ggagaaatac
     1741 accactgctc catttttggc aggacacgat ttaaagtacg ttggcgacgt agttaccccc
     1801 tggcccggct ttgttctggc ggcttccatt aatatgtgcg aaagccatta tcaggatctg
     1861 cagcttattg ggaaaactat tgcaagcgtt tgcaggagcc ttagaaatga tcatcaaatc
     1921 gctgaaaaaa ttgcgaaaaa acaccattta gatgttgctc aggttgagct ttggctgcat
     1981 gaacttgaat ggaatgagga ccaaggcatc tccaaaggcg aacaaaccac aattttgcat
     2041 ggtctttgca atgcaggaat aataaaagaa acagcaattc cacatcctga aaaatttttt
     2101 cttgaatgat aatcctaaga tccttacttc tcctatcctt tatagcgaca tgcctcaatg
     2161 cccgctcgca ggaaatgatt gccgacagtt catggagaat ccccgataat ctcgccgaaa
     2221 catccggact ttcacttttt ggaatgaatt tgctatcgca taatgacagc agtcacccca
     2281 acctactgat tgaaatgaat cgcaaaggag gatttgtgcg cacactaaca gttgagcaat
     2341 ctaaaaatgt cgactgggaa gaactgtgc
//
LOCUS       DAOMYG010000471         2364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_17238_length_2364_cov_6.564747, whole genome shotgun sequence.
ACCESSION   DAOMYG010000471 DAOMYG010000000
VERSION     DAOMYG010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17238_length_2364_cov_6.564747"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /locus_tag="PK610_12660"
     CDS             <1..1535
                     /locus_tag="PK610_12660"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ36837.1"
                     /translation="STAGIEVGLAWNFRMKKKPQPIQENYLAKRSPYMNTIFDELNPI
                     VSPDGRTLYFSRNNDVRNTNGIDDSQDMWRADVSGGTDSAKAVHLSTPFNQLKYNYMA
                     GITPDENTIMISGAFVNGKMEGKGYSLTHRTRDGWSEPKKMQIEGFGELVKGAYAGAF
                     LSNDGQHLVQYFSETTGTESYDLYVSHLKEDGTWTRPQKLGNLNTKNNEMSPFLASDG
                     VTLYFSSDRSGGLGSNDIYVTKRLDDTWTNWSEPKNMGSDVNSDKWDAYYTIDAQGKY
                     AYMVSYKNAGMGSDIVRIKLKEDVQPDPVVLVKGRVLNAKTKEPLEASVAYNSLNGKT
                     QNGIARTNPATGEYKIVLPYGSEYSFLGEAKNFVAQSNNLDLNQKGQYREIEMDIYLY
                     PIEVGSTVRLNNIFFETGKATLTEKSTAELDRLVKILNENPNVEIEIGGHTDNVGAAA
                     FNKQLSAERAAAVVTYLVSKGVNKSRLKSVGYGMDRPAGENNTEEGRAYNRRVEFTIT
                     KN"
     gene            complement(1643..>2364)
                     /locus_tag="PK610_12665"
     CDS             complement(1643..>2364)
                     /locus_tag="PK610_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36838.1"
                     /translation="LLLYSIMFLLNGDWKKWSIYLALLTGILLLLYLKVYVLMALLPG
                     IIGFVFVRKTSVQLLFLKYGLVVLVCLFGWVYFEKLFPSWHMLEILVQKQQDFIRLSQ
                     AMNSGSMLYTVPLDPDFLSFIKAAPLAFTNVLLRPFPGESFSPFLLIASAENAILLVA
                     MVLVVFRFQKPNKDQLNLIVFFLLFVVGLFVLIGWVTPVMGAIVRYKVPALPFLGMIV
                     VMLMNIQGKPSSENYSAKRSD"
BASE COUNT          819 a          469 c          478 g          598 t
ORIGIN      
        1 tttcaactgc aggaattgaa gtaggtcttg catggaattt cagaatgaag aaaaagccac
       61 agcctattca ggaaaattat cttgctaagc gttctcctta catgaataca atttttgatg
      121 aactcaatcc aatcgtttca ccggacggac gtacacttta tttttcaaga aataatgatg
      181 taagaaacac caacggaatt gacgattcgc aggatatgtg gagagctgat gtgtcaggcg
      241 gaacagacag tgcaaaagcg gtacacttat ctacaccttt caatcagcta aagtacaatt
      301 acatggcggg tattactccc gatgaaaaca ccattatgat ttcaggtgct tttgtaaatg
      361 gaaaaatgga aggcaaaggt tattcattaa cacaccgtac ccgcgatgga tggtctgaac
      421 cgaagaaaat gcaaattgaa ggatttggcg aacttgtaaa aggtgcttat gccggtgcat
      481 tcctttcaaa cgatggtcaa catcttgttc agtatttttc tgaaactaca ggaacagaaa
      541 gttatgattt gtatgtgtcg catcttaagg aagacggaac atggactcgt ccgcaaaaac
      601 tcggtaattt gaataccaaa aacaacgaaa tgtctccttt ccttgcatca gacggagtga
      661 ccttgtattt ctcttctgat cgttcaggtg gattgggaag caatgatatt tatgtgacca
      721 agcgtcttga cgatacctgg accaactggt cggaacccaa gaacatggga tcggatgtga
      781 attctgataa atgggatgcc tattatacca ttgatgcgca aggaaaatat gcttacatgg
      841 tttcctacaa aaatgcaggc atgggttctg atattgtgcg catcaaatta aaagaagatg
      901 ttcagcctga tccggtagta cttgtaaaag gacgtgtatt aaatgcaaaa acaaaagaac
      961 cacttgaagc aagtgttgct tacaattcat tgaacggtaa aacgcaaaat ggtattgcac
     1021 ggaccaatcc agcaaccggc gaatacaaaa ttgttttgcc ttatggttca gaatattcat
     1081 tcttagggga agcgaaaaac tttgtagctc agtcgaacaa ccttgatttg aatcagaaag
     1141 gccagtatcg cgagattgag atggatattt acctctaccc gattgaagtt ggttctacag
     1201 tacgtttgaa caacattttc tttgagacag gtaaagcaac tctcactgaa aaatcaaccg
     1261 cagaacttga ccgtttggta aaaatcctga atgaaaatcc aaatgtggaa attgaaattg
     1321 gtgggcatac cgacaatgtt ggtgctgcag cattcaacaa gcaactttct gctgagcgtg
     1381 cagctgcagt tgtgacctac ctggtttcta aaggcgtaaa taaatcgcga ctaaaatctg
     1441 tcggttacgg aatggatcgt ccggccggag aaaacaacac tgaagagggt cgtgcctata
     1501 atcgtcgtgt tgaattcaca attaccaaaa actagtcaat aacatcaaat gaggaaagaa
     1561 gtccctcgtc ggaaacggcg ggggattttt tttgtcacta tacaaaaccc tgaagaaaag
     1621 aaagagtcaa cactacatca aatcagtcag aacgctttgc tgaatagttt tcagaagaag
     1681 gtttaccctg aatattcatg agcattacca ctatcattcc caggaacgga agagcgggaa
     1741 ctttgtaacg aacaattgcc cccattaccg gagtaaccca tccgattaaa acgaataatc
     1801 cgactacaaa caacaaaaag aaaacaatca gattcaattg gtctttgttt ggtttctgaa
     1861 agcgaaaaac aactaaaacc atcgcaacga gaagaattgc attctcagct gaggcaatca
     1921 gtagaaacgg agaaaaggat tcacctggaa atggtcgtaa caaaacattt gtaaaagcca
     1981 gtggcgctgc tttaataaaa cttaaaaaat ccggatccaa gggcacggta tataacatac
     2041 ttcccgaatt cattgcctga gaaagtcgaa taaaatcctg ctgcttctga accagaattt
     2101 cgagcatgtg ccaggatgga aataacttct caaagtaaac ccagccaaac aaacacacca
     2161 atactacaag tccgtacttc aaaaaaagta attggacaga agttttcctg acaaaaacaa
     2221 aaccgataat tcctggcaac aaggccatca gcacatagac cttcaaataa agcaacaaaa
     2281 gtattcccgt taaaagtgct aaataaatgc tccacttttt ccaatctcca tttaacaaaa
     2341 acattatcga atacagaagt aatc
//
LOCUS       DAOMYG010000472         2359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_17293_length_2359_cov_6.907986, whole genome shotgun sequence.
ACCESSION   DAOMYG010000472 DAOMYG010000000
VERSION     DAOMYG010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17293_length_2359_cov_6.907986"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="PK610_12670"
     CDS             <1..1543
                     /locus_tag="PK610_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36839.1"
                     /translation="SAIDNKGNIGDGQLGVFMDLVADLRESKTEAYSGAINPNIDAFV
                     LPIPVPVVIPTIHANVQQEKTQFRSATTTKVIQRSGILKETIATDLGSVVSTQNLLYD
                     RETGEILLTSTKTNFNDLVYSLTVPGHWYYDDMGPAYKNIGYTISSPVTFNTSGTATI
                     ANASNYFVEGDELMILGYGIGWVTTVSANSITVVRKTGVPAIGTFSGLSVLRSGRRNQ
                     ASTPIAAVVSLSDPRTGIKTNSFSKVLSSSAIEFGEVWRTYCDCFDGNDDQFAYSTNP
                     YILGRKGNWKPLRSWTHLAGRTQSTYNNNTNIRRDGIFTSYTPFYKFTPNGWRMDNFN
                     WTYVSEVTEFNPFGSEIENKDALGRYSASQLGYRQTQPIAVAANAKYAEIGFDGFEDY
                     GFSPCADAHFKFDNPVLSTAASHSGRYSVKVSSGTDLVLTKAFEACLIQDCTTEIGTT
                     TGEGYIIVTVSGQGSGAEFDYEVIQGDPVINIVSGQFRVYGTGWTIKVIIREPGCLTS
                     KIISQ"
     gene            1553..>2359
                     /locus_tag="PK610_12675"
     CDS             1553..>2359
                     /locus_tag="PK610_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36840.1"
                     /translation="MKAFYILLFVATGTKAFASLPPQACAEAQFFCAKEFTYNYSVNN
                     CTDTTELWFYFNAGSTVAEMSIASNDSIYGYTIMGPFAGALTQPCNALDSTTTIDFTV
                     SSTKILHQITMDLGGSLPVGYYYVRVKAKSCKSSISFISKNEYLTCPEIPCENCIGSF
                     QLEPGKKYVLSLWTKESGAAPTKTSYDRPSVQVGFPSLSLMLTSVYPTGQIIDGWQRI
                     ETEFTVPISATNFQLKLSCSTGDCYFDDLRIHPFEGSMKTYVYDPVNLRLV"
BASE COUNT          697 a          460 c          503 g          699 t
ORIGIN      
        1 atctgcaatt gataataaag gaaacattgg agacggtcag ttgggagtgt ttatggactt
       61 ggttgcggat ttacgcgagt cgaaaacaga ggcttatagc ggtgcaatta acccgaatat
      121 tgatgcattt gttttaccaa ttccagttcc agttgtaatt cctactattc acgcgaatgt
      181 tcaacaagaa aaaacccagt tcagaagcgc aactacaact aaagtaattc agcgttccgg
      241 tattttaaag gaaactattg ccacagatct tggatcagta gtttcaacgc agaatttact
      301 ttatgacaga gaaaccggag agattttact cacctccaca aaaaccaatt tcaacgattt
      361 ggtttattct ctcacagttc caggacattg gtattatgat gatatgggcc cggcttataa
      421 gaatattgga tacaccatct caagccccgt tacatttaac acttccggta cagcaaccat
      481 tgccaatgcc tcgaattatt ttgtagaggg tgatgagcta atgatcttgg gttacggtat
      541 tggttgggtt accacagtaa gcgcaaattc cataactgtt gtcagaaaaa caggagttcc
      601 ggcaattggt acatttagcg gtttaagcgt gctgcgttcc ggtcggagaa atcaggcatc
      661 tactcctatt gcggctgtgg tttccttatc cgatccaaga acaggtataa aaacaaatag
      721 tttttctaaa gtactgtctt cctctgccat tgaatttgga gaggtctgga gaacatactg
      781 cgattgcttc gacggaaatg atgatcagtt tgcgtattca accaatcctt atattcttgg
      841 aagaaaagga aactggaagc cgctccgttc atggacacac ctcgccggca ggacacaatc
      901 cacttataac aataatacca acatccgcag agatggaatt tttacaagct atacgccatt
      961 ttacaaattc actcccaatg gatggcgaat ggataatttt aattggacct atgttagcga
     1021 ggtaacagaa ttcaatcctt ttgggtcaga aatagaaaat aaagatgccc tgggtcgtta
     1081 ttctgcttcg cagttgggtt atcggcaaac acagcccatt gctgtagctg caaatgctaa
     1141 atatgcagaa attggatttg atggttttga agattacggt ttcagtccat gtgcagatgc
     1201 tcattttaaa ttcgataatc cggtgttgtc tactgctgca tcgcattcgg ggaggtacag
     1261 tgtaaaagta agttcaggta ccgacttggt gctgaccaaa gcatttgaag catgtttaat
     1321 ccaggattgt acaaccgaaa ttggtacaac aacaggagaa gggtacataa ttgtaactgt
     1381 ttccggacaa ggatcagggg ccgaatttga ttacgaggtt attcagggtg atccggttat
     1441 aaatattgtt tccggtcaat tcagagtcta tggcaccgga tggacgataa aagtgattat
     1501 ccgcgaaccg ggttgtttaa ccagcaagat tatttcacaa taaattttga agatgaaagc
     1561 attctatatc ctactttttg tagccaccgg aaccaaggct tttgcaagtc ttcctcctca
     1621 ggcttgtgca gaagctcagt ttttctgcgc aaaggaattt acttacaatt attcggtgaa
     1681 taattgtaca gatacaacag aattatggtt ttattttaat gcaggaagca ctgtagctga
     1741 aatgtcaatt gctagcaatg attcaatcta cggctatacc atcatgggcc cctttgcagg
     1801 agctttgact caaccatgta atgcgctgga ttctacaaca actattgatt ttacggtttc
     1861 atcaacaaaa atactgcacc agatcacaat ggatttgggt ggttctttac ctgtaggata
     1921 ttattatgtg cgagtaaaag caaagtcgtg caaatcgtcc attagtttta tttcaaagaa
     1981 cgaatacctt acctgcccgg aaattccctg cgagaattgt attggctcat ttcagctgga
     2041 accgggaaaa aaatatgtgc tgagtttgtg gaccaaggaa tcaggtgctg cgccaacaaa
     2101 gacatcgtat gaccgtccct ccgttcaggt tggatttcct tctttaagtc tgatgttaac
     2161 aagtgtttat cctacaggac aaattatcga tggttggcaa cgaatagaaa cagaatttac
     2221 tgtgcctatt tccgcaacaa attttcagtt gaagctaagc tgttctacag gtgattgcta
     2281 tttcgatgat ttacgtattc atccattcga aggcagtatg aaaacctacg tttatgaccc
     2341 tgtcaattta cgtttagta
//
LOCUS       DAOMYG010000473         2357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_17311_length_2357_cov_7.716334, whole genome shotgun sequence.
ACCESSION   DAOMYG010000473 DAOMYG010000000
VERSION     DAOMYG010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17311_length_2357_cov_7.716334"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PK610_12680"
     CDS             complement(<1..443)
                     /locus_tag="PK610_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36841.1"
                     /translation="MRVVFSLCLSLLLFNASAQKKKVREDYFQQEVQYSIDVKLDDEN
                     HTLTGFERLVYINRSPQTLSQLYFHLWPNAYRNNETALAKQLLENGETAMHFANPQGE
                     LGFIDSLQFIVNKDTIRWEYDSTHMDIVVLHLNKLLKPGDTAIITT"
     gene            complement(443..1621)
                     /locus_tag="PK610_12685"
     CDS             complement(443..1621)
                     /locus_tag="PK610_12685"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRJ36842.1"
                     /translation="MKNLFSLILFICCTALQAQQPAKPVFKETTVSGTITNAPGKKLY
                     LENFERSGFASRMDSTIINKKGKFAFKMKIDKTNYYRFAFQSNDFFVLILQPGEKVTV
                     IADGNRMNQSYKVVGSEHSVKLQDFVKLVNDYIQERDSIQAKVKEFAQQGDQNNANIA
                     NTELNNAYTRFLGNRDKFLNDNPASPALLGVLNHLNQNQDIEQMRKIENALGASMPGS
                     AFHESVKSMRMNLEAKLAEEERKRKEQEEFANRLAPGKLAPPIEMNDANGKLLPLSSL
                     KGKVVLIDFWASWCGPCRKENPNVVRVYNKYKDKGFTIYSVSIDQSKENWLAAIEKDQ
                     LSWPSHVSTLQGWQTPILRDYGVNGVPFTVLIDRDGKIIQANLRGPMLEAKLAEIFGF
                     "
     gene            complement(1618..>2357)
                     /locus_tag="PK610_12690"
     CDS             complement(1618..>2357)
                     /locus_tag="PK610_12690"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRJ36843.1"
                     /translation="AVAIDDSVNRNKILAEVEIAKTNFNNYKYDFVDKNPNSPALVTT
                     MNQFDPVGEIDYFKKIESALSVSMPNSEYHLAVKQTIEQAQFQKQMMEMQKAEEERLA
                     NILKPGTAAPEIDLPGVDGKNKKLSSLRGKYVLIDFWASWCAPCRRENPTVVALYNKY
                     NGRGFEIFSVSLDKEKDKWINAIREDGLIWPSHVSDLKQWETSVIGTYQFSSIPFTVL
                     IDKQGNVISTNLRGTQLEDKLRELFGS"
BASE COUNT          643 a          472 c          470 g          772 t
ORIGIN      
        1 gttgtgataa tggcggtgtc accaggtttg agcaatttat tcaagtgcaa aaccacaatg
       61 tccatgtggg tggaatcata ttcccatcta attgtatcct tattgacaat gaattgtaat
      121 gagtcaataa atccgagttc gccttggggg ttagcaaagt gcattgctgt ttcaccgttt
      181 tcaagaagct gtttagccaa tgctgtttca ttgtttctat atgcatttgg ccacaaatga
      241 aaatacaact gcgaaagtgt ttgtggagat cggttgatgt aaacaagccg ttcgaaacca
      301 gttaaggtat gattttcatc atctagcttt acatcaatgg agtattgaac ttcttgttgg
      361 aagtaatctt ctctgacctt ctttttttgt gcgcttgcgt tgaataacaa caacgaaaga
      421 caaagagaaa aaaccactcg catcagaatc caaaaatttc tgcaagttta gcttccagca
      481 taggtccgcg taaattggcc tggataatct ttccgtccct gtcgatgaga actgtgaacg
      541 gaacaccgtt tacaccgtaa tcacgaagaa taggcgtttg ccaaccctgc aatgtgctta
      601 catgactggg ccagctgagt tgatcttttt cgatggcagc taaccaattt tccttgcttt
      661 gatcaattga aacgctgtaa atagtgaatc ctttgtcttt gtatttgtta tagacacgca
      721 ccacgttagg attttctttt ctgcatggtc cgcaccaaga agcccagaaa tcgataagca
      781 caacttttcc ttttagcgat gataatggaa gtaattttcc attggcatca ttcatttcaa
      841 ttgggggggc aagttttccc ggggccagtc gattagcaaa ttcttcctgt tcttttcgtt
      901 tacgttcctc ttccgcaagt ttcgcttcaa gattcatacg catagatttc acagattcat
      961 gaaacgcaga tcccggcatt gaagcgccaa gcgcattttc aattttgcgc atctgttcga
     1021 tatcctgatt ttggttaaga tgattcaaaa caccaagaag agcaggggaa gccggattat
     1081 cattcaaaaa tttatcacgg ttgccaagaa agcgtgtata ggcattgttt aactcagtat
     1141 tggcgatgtt ggcgttattc tgatcacctt gttgagcaaa ttctttaact tttgcctgta
     1201 ttgaatcccg ttcctgaata taatcgttca caagttttac gaaatcctgc aattttaccg
     1261 agtgctcaga acctaccact ttatacgatt ggttcattcg gtttccatct gcaatcacag
     1321 ttactttttc tccgggttga agaatgagca caaaaaaatc attcgactga aatgcaaaac
     1381 gataatagtt tgttttatca attttcatct tgaaagcgaa ctttcctttt ttattgatta
     1441 ttgtactatc catccgcgat gcaaacccac tacgctcaaa attttcgaga tacagctttt
     1501 tccccggcgc gttggtaata gtgccggata cagtagtttc tttaaatact ggttttgccg
     1561 gttgctgagc ctgaagtgca gtacagcaaa taaacaggat tagtgaaaat agatttttca
     1621 tgagccgaaa agttctctta gtttatcttc aagttgcgtg ccacgaagat tggtggaaat
     1681 aacatttcct tgtttatcaa tgagcacggt aaaaggaatt gagctgaatt ggtaggttcc
     1741 aattacagat gtttcccatt gctttaaatc gctgacatgc gatggccaaa tgagaccatc
     1801 ttcacgaatt gcattgatcc atttgtcttt ttctttgtcg agcgaaaccg aaaagatctc
     1861 aaatccacga ccgttgtatt tattgtaaag tgctactaca gttggatttt ctcttctgca
     1921 tggagcgcac catgaagccc agaaatcaat tagtacatat tttccacgga gagaagaaag
     1981 ctttttgttt ttcccatcta ctcccggtaa atctatttca ggggcagctg tacctggctt
     2041 gagaatgttg gctaaacgct cttcttcggc tttttgcatt tccatcatct gcttttgaaa
     2101 ctgagcttgt tcgatggttt gctttacggc aagatggtat tcgctgttgg gcatcgatac
     2161 agaaagagca gactcaattt ttttgaaata gtcgatttct ccaacaggat caaactgatt
     2221 cattgtagta accaacgcag gagaatttgg gtttttgtcg acgaaatcgt atttgtaatt
     2281 gttaaagttt gtttttgcaa tttctacctc tgcaagaatt ttatttctgt taacggaatc
     2341 atcaatggca accgcca
//
LOCUS       DAOMYG010000474         2352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_17369_length_2352_cov_8.121898, whole genome shotgun sequence.
ACCESSION   DAOMYG010000474 DAOMYG010000000
VERSION     DAOMYG010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17369_length_2352_cov_8.121898"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1807)
                     /locus_tag="PK610_12695"
     CDS             complement(20..1807)
                     /locus_tag="PK610_12695"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ36844.1"
                     /translation="MSKKNKHEKHQHGQKKQTESTVVSPLAVNLPSRSTLRMTAFFLM
                     LIGVVLYANTFNHGFVLDDHSVIRDNKLTTQGLSATKEFFKSGLRTGNFLVEDNLYRP
                     LTKTFFAAQWQIAPDNASFFHGVNILLYGFLCALLFLTWMRLMPGKYYVALIGTLIFA
                     FHPLHTEVVANIKSADEILSFIFLLFSIQAAVSYVEKKSILWLFASAALYFLGLLTKE
                     NAITFLALLPVTLWFFTNAKRKEYISTILLFSVVTVVYLYIHRSVIGMIGLENVPVVD
                     NSLFATKSFLSQRITAIYILGLYLRLLLIPHPLSCDYSFNTIPNIETIANGGFLLSFA
                     IHVFALWYAIRYFKSKNIGAYAIWFYLISISVVSNVIVLIGTNMGERLVFYPSAGFTL
                     LIAFVMDKFIQFPKDKFSVPALFSKVPVIAILAPVLLFYGFKTIDRNKDWESDATLFA
                     ADVKTVPNSAHMLYYHAGVLADRDSLAFFTPDVKMYRLQQAEQILVRSIAIYEPFPNV
                     HSLIGRVYKDMGNYEKAIYHYERTLQLNDKDPTTHNNLATCFFETGKLPKAENHFSRA
                     IELSPFCYDDAICNLGSVYGTMGAAGRAD"
     gene            complement(1854..>2352)
                     /locus_tag="PK610_12700"
     CDS             complement(1854..>2352)
                     /locus_tag="PK610_12700"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRJ36845.1"
                     /translation="LFLEEVKEEPFRIDRMFAKPEDEVKVVRAGKAEGILFGGNLSVL
                     CGMIGTPYFPELKGAILFLEEVKEEPFRIDRMFAQLRLAGAFEKINGLIIGKFRDCIP
                     EEPDFAFSSDEVIQQYFANAPFPVISNAMIGHIADKFTIPVGIRAQLDSESKSFRLLE
                     PAVLT"
BASE COUNT          769 a          486 c          470 g          627 t
ORIGIN      
        1 ggcgacgagg aagccttctt caatctgctc ggcctgctgc acccatcgtt ccgtaaacac
       61 ttccgagatt acagattgca tcgtcataac aaaacggact caattcaatt gctcttgaaa
      121 aatgattttc tgctttagga agtttcccgg tttcaaaaaa acatgtggcc agattgttat
      181 gcgtggtagg atctttatcg ttgagctgta aggtgcgctc ataatgataa atcgcctttt
      241 cgtaatttcc catgtcttta taaacgcgtc cgattaacga atgtacgttg ggaaatggct
      301 cataaatggc aatagaacga accaggatct gttctgcctg ttgcaagcgg tacatcttta
      361 catcgggtgt aaaaaaggcc agcgaatctc tgtcggccaa cactcctgcg tggtaataca
      421 acatgtgtgc agaattcgga actgttttta catcggctgc aaaaagtgtt gcatcgcttt
      481 cccaatcttt attgcgatcg attgttttga atccgtaaaa caacaagacg ggtgctaaaa
      541 tggcaatgac aggaacttta ctgaaaagtg cgggaacaga aaacttgtct tttggaaact
      601 gaatgaactt atccattaca aacgcaatta acaatgtaaa tcctgcagaa ggataaaaca
      661 ccagacgttc acccatattg gtaccaatca gcacaatgac attcgaaacc accgagatgg
      721 agatcagata aaaccaaatg gcatatgctc cgatgttttt gcttttgaaa taacggatgg
      781 cataccacaa tgcaaacaca tgaattgcaa acgaaagtaa aaaccctcca ttggcgattg
      841 tttcaatatt aggaatggtg ttgaacgaat agtcacagga caaaggatgc ggaataagca
      901 ataaacgcaa atacaaacca agaatataga ttgcagttat tcgctgactt aaaaagcttt
      961 ttgtagcaaa caaggaatta tcgacaacag gaacattctc cagcccaatc attccgatta
     1021 cacttctatg gatgtacaga tacacaactg taacaacaga aaaaagcaga atagtactga
     1081 tgtattcttt cctttttgca ttggtaaaaa accaaagggt tacaggcaat aaagcaagga
     1141 aagtaatggc gttctccttg gtgagtaatc caagaaaata caatgctgca gaagcgaaca
     1201 accagagaat tgattttttc tccacataag atacagcagc ttgaatagaa aacagcagga
     1261 atataaagct tagaatttca tccgcacttt ttatgttggc aacaacttca gtatgcaaag
     1321 gatgaaaagc aaaaatgagt gttccgatca aggcaacata atatttacct ggcatcaaac
     1381 gcatccaggt caaaaacaag agcgcacaca aaaaaccgta caacaaaata tttaccccat
     1441 gaaaaaatga agcattatcc ggagcgattt gccattgagc agcaaaaaat gttttggtaa
     1501 gaggtcggta caaattatct tcaaccagaa agtttcctgt gcgtaaacca cttttaaaaa
     1561 actctttggt agccgacaat ccctgcgtgg tgagtttgtt atcgcgaatc accgaatgat
     1621 cgtcaagcac gaaaccgtgg ttaaacgtgt ttgcatacaa cactacgcca atcagcataa
     1681 gaaaaaaggc ggtcatcctg agtgtcgatc gtgaaggaag attgacagcc agcggtgaaa
     1741 caactgtgct ttcagtttgt tttttctgac cgtgttgatg cttttcgtgt ttgttctttt
     1801 tactcatgac ggtaggatat aatatcaaaa ataggaattc tgaaataaca ggtttaggtc
     1861 agcacagcgg gttccaacaa gcgaaaggat ttggattccg aatccagctg agccctgatt
     1921 ccaacgggta tggtgaactt atcggcaata tgtccgatca tagcattgct gattaccgga
     1981 aaaggagcat ttgcaaaata ttgttgaatg acctcatccg aggaaaatgc aaaatccggt
     2041 tcttccggaa tgcagtctct gaatttaccg atgattaatc catttatttt ttcaaatgca
     2101 cctgcaagtc gtaattgtgc aaacatacga tcgatgcgaa acggttcttc ttttacttct
     2161 tcaagaaaga ggattgcacc tttcagctcc gggaaatacg gtgttccgat cattccgcac
     2221 aacacactta aatttccgcc gaacaaaatg ccttcagcct ttcccgcacg aacaactttc
     2281 acttcatctt ccggttttgc aaacatacga tcgatgcgaa acggttcttc ttttacttct
     2341 tcaagaaaga gg
//
LOCUS       DAOMYG010000475         2347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_17436_length_2347_cov_8.065445, whole genome shotgun sequence.
ACCESSION   DAOMYG010000475 DAOMYG010000000
VERSION     DAOMYG010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17436_length_2347_cov_8.065445"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="PK610_12705"
     CDS             <1..1078
                     /locus_tag="PK610_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRJ36846.1"
                     /translation="FMSKFHSLTVVDIRKETSDSVSVAFEVDAKDSAAFSYAPGQYLT
                     LRFTINGEEVRRSYSLCSSPFSGEKMRVAVKIVQGGKVSTYMNSVLKVGDKVDSLLPM
                     GSFTTTLKSDNQKHYVGFAAGSGITPVYSLIKAILEKEPKSKFTLIYSNKGSESTIFN
                     KELEDLCDANANRLKVVHVWSRQETGDPILNGRLDAERTAAVISKFELKAADEFFICG
                     PEEMIMNVSKSLENSGIKKEAVHFELFTTPVLMASTESSVSAETFSGDSQVTVIMDGI
                     ETKFKLAGNGVNILDAAIDAGADAPYSCKGAVCCTCKAKVQEGKAAMDMNYALTDKEV
                     ADGYILTCQAHPLTDKLVVDYDVA"
     gene            1081..1812
                     /locus_tag="PK610_12710"
     CDS             1081..1812
                     /locus_tag="PK610_12710"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRJ36847.1"
                     /translation="MHLALFIALTLLAEILGTLGGFGSSALFVPLCGFFFGFQTVLAF
                     TGILHVFSNGAKLWLFGKHLNLRIFFWLGIPSLIFSVIGAFLIVGTNSEWSQIVLGIF
                     LVSGSSLLMLWPNIQMRDKFSNHLLAGSTAGFLAGFTGTGGPVRALALSAVGLSKESF
                     IASSAAIDMAVDLPRSLIYLSAGYLDPELYWYIPILACIAFLGSWLGKLLLDRVTSRF
                     FRSLVLFLVFAIGSMMIIKTIFYDA"
     gene            1802..>2347
                     /locus_tag="PK610_12715"
     CDS             1802..>2347
                     /locus_tag="PK610_12715"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ36848.1"
                     /translation="MTLEFFGAAECVTGSKHLITLSDDTKILLDCGMFQGMGKETEPL
                     NRHFGFDPSSIHALILSHAHIDHSGLIPRLIKEGFRGKIYCTDATFDLCKVMLKDSAK
                     IQEYDIMYLNKKRKERKQKLLEPLYTEDDVNFCLQMFETRDFETPFDVTKNVKCVFTD
                     NGHILGSAAVYLKINEEETITR"
BASE COUNT          656 a          415 c          525 g          751 t
ORIGIN      
        1 gtttatgtca aaattccatt cccttacagt tgttgatatc cgtaaagaaa cttcggacag
       61 tgtttcagtt gcgtttgaag ttgatgcaaa agattctgcc gcattctcct atgctcccgg
      121 gcaatatctt accttaagat ttacgataaa tggggaagaa gtccgtcgtt cgtattcact
      181 ttgttcttct cccttttccg gagaaaaaat gcgtgtggca gtaaaaattg tgcagggtgg
      241 aaaagtttca acctatatga attctgtgct taaggttgga gataaagtag attccctcct
      301 accaatgggg agttttacaa caacattaaa atcagataac cagaaacatt atgttggttt
      361 tgcagcagga agcgggatta caccggtgta ttcacttata aaagctatcc tcgaaaaaga
      421 acccaagagt aagtttacgc tcatttattc taataagggg agtgaatcaa caatctttaa
      481 caaggaactg gaagatcttt gcgatgcaaa tgctaaccgt ttaaaggttg ttcatgtatg
      541 gagcagacag gaaacaggag atccgatttt gaatggcaga ctcgatgctg aacgtacagc
      601 tgcagttatc agtaaatttg agctcaaagc tgcagatgaa tttttcattt gtggtcctga
      661 agaaatgatc atgaatgttt ccaaatcatt ggagaattct ggaattaaaa aggaagctgt
      721 tcattttgaa ttattcacaa ctccggtatt aatggcttcc actgaatctt ccgtttcagc
      781 agaaacgttt tctggggata gtcaggtaac ggtgatcatg gatggcattg aaacgaaatt
      841 taaattggcc ggaaacggag tcaatattct ggacgcagcc attgatgccg gtgcggatgc
      901 tccttattcg tgtaaagggg cagtttgttg tacctgtaaa gcaaaagttc aggaaggtaa
      961 agcagctatg gatatgaatt atgctcttac tgataaagaa gtggccgacg gatatatttt
     1021 aacatgtcag gctcaccctt taactgataa gctggttgtc gattacgacg ttgcctgatt
     1081 atgcacttgg cgctttttat tgcactcacc cttcttgctg aaattttagg aacactggga
     1141 ggatttgggt caagcgcatt gtttgttccg ctgtgcggtt ttttcttcgg ttttcagacg
     1201 gtcctggcat ttacaggtat tttgcatgtg tttagcaatg gtgcaaagct ttggctgttt
     1261 ggaaaacacc tgaatctgcg tatatttttt tggctgggta ttcccagttt aatcttttca
     1321 gtgattggtg catttttaat tgttggaact aatagtgaat ggtcgcagat agtacttgga
     1381 attttcctcg tttccggcag ctcattattg atgctttggc cgaacattca gatgcgggat
     1441 aaattttcca atcatttatt agcagggagc acggctggtt ttcttgcagg ttttacaggg
     1501 actggtggac cggtaagagc acttgcattg tcggctgttg gtttgagcaa ggaaagcttt
     1561 atagcctcat ctgcagcgat tgatatggca gtggaccttc cacgaagttt gatttatttg
     1621 tcagcaggat atttagatcc tgaactctat tggtatatcc ctattcttgc atgtattgca
     1681 tttttgggat catggttagg aaaattactt ctggataggg ttacaagcag attttttcgt
     1741 tccttggttt tattccttgt gtttgcaatt ggaagcatga tgattattaa aacaatcttt
     1801 tatgacgctt gagttttttg gagctgctga atgtgttaca ggtagcaagc atttaataac
     1861 cttgtccgac gatactaaaa ttcttttgga ttgcggaatg tttcagggaa tgggcaaaga
     1921 aacagaacct ctcaaccgcc atttcggatt tgatccttcc tctattcacg cgctcatcct
     1981 atctcacgcg catattgatc attccggact cattccacgg ttgataaaag agggattcag
     2041 agggaaaatt tattgtacag acgccacctt cgacttgtgc aaagtcatgc ttaaggattc
     2101 agcaaaaatt caggagtatg atattatgta cctcaataag aagagaaaag aacgcaagca
     2161 aaaattgctg gaacctctct atactgaaga tgatgttaac ttttgtcttc aaatgttcga
     2221 aacaagagat tttgaaactc cttttgatgt cacaaaaaat gtaaaatgtg tatttaccga
     2281 taatggtcac attctcggta gtgctgccgt ataccttaaa atcaatgaag aagaaacgat
     2341 tacacgt
//
LOCUS       DAOMYG010000476         2345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_17463_length_2345_cov_9.558079, whole genome shotgun sequence.
ACCESSION   DAOMYG010000476 DAOMYG010000000
VERSION     DAOMYG010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17463_length_2345_cov_9.558079"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PK610_12720"
     CDS             <1..403
                     /locus_tag="PK610_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRJ36849.1"
                     /translation="PLIGKNDENLGPRFPDMSEPYDKSLLKNALEIAKKHSIRVKQGV
                     YAGLSGPCYETPAEYRYLRIIGADAVGMSTVPEVIVARHSGMKCFAVSIITDLGIDGK
                     IVEVSHEEVQKVAAESEPLMTLIIRELLGSL"
     gene            450..1916
                     /gene="gatB"
                     /locus_tag="PK610_12725"
     CDS             450..1916
                     /gene="gatB"
                     /locus_tag="PK610_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRJ36850.1"
                     /translation="MSSIYDNYEAVIGLEVHAQLLTKSKNFSPDRNEYGAMPNTNVSV
                     ISIGHPGTLPKLNKRSIEFAVRLGLACKSTIRSEMHFARKNYFYADLPKGYQITQDNT
                     PICTEGEIIIKDDQGNDKRIGLIRIHIEEDAGKSIHDVDPFDTLVDLNRAGVPLLEIV
                     SSPDMRTGKEAYNYLTEVRKLVRYLDICDGNMEEGSMRCDANISVRKKGSQEFGTRTE
                     TKNINSIRNVQRAIEFEIKRQIDVLEAGGTVVQETRSFDAPTGGTISMRSKEMAHDYR
                     YFPEPDLPPVKVSQEYIANVKAALPPLPAELFLRYTKELGLPEYDAYQLTDHKPIALF
                     FEELIKHTGNYKSAANWIMGEVRSWLNENGLEIEDFPLPASRIAEIIALIDASKVSHT
                     VASQTIFPALIANPEKTALRIAEEKNLIQESDSGALIGFIHEAIKNIGPDKLAEYKGG
                     KKGLVGLYMGEVMKLSKGKADPKSTNQLLVKTLDETTL"
     gene            1919..>2345
                     /locus_tag="PK610_12730"
     CDS             1919..>2345
                     /locus_tag="PK610_12730"
                     /inference="COORDINATES: protein motif:HMM:NF025647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HRJ36851.1"
                     /translation="MKQVFTLLIFATVMISCGGGGNQISGSFTNGSGKTIYLERFEKS
                     MPVVVDSAKLADDGSFSLTFPNKTELYRLRIQPNDFAVLILDSTDKPEIKGDANQIYA
                     TYTISGARNSELIQSFFKRTNEYMIERETLRQKLMAVAID"
BASE COUNT          725 a          478 c          527 g          615 t
ORIGIN      
        1 tccgcttatc ggtaaaaacg atgagaattt aggcccgcgt tttccggaca tgtcggagcc
       61 ctatgataaa tcgcttctta aaaacgcatt ggaaattgct aaaaagcatt cgattcgtgt
      121 gaagcaaggg gtatatgcgg gtttaagcgg tccgtgctat gaaacaccgg ctgagtaccg
      181 ttatctgcgc atcattgggg cagatgctgt tggaatgagt accgttcctg aagtaatagt
      241 tgcccggcac tccggcatga agtgttttgc ggtctcaata ataactgacc tgggaattga
      301 tggtaagatt gtggaagtga gccatgaaga ggtccagaaa gtggcagcag aatcagagcc
      361 cctaatgact ctcattatcc gtgaactact cggctcactc tgattaatca gggttttatt
      421 tttcgtacct ttagccggtc aatttcccta tgagtagcat ttacgataat tacgaagcag
      481 taatcgggct tgaggtgcat gcacagctgc tcaccaaaag caaaaatttt tctcccgatc
      541 gaaacgaata cggcgctatg ccgaacacca atgtgagcgt gatctctatt ggacatcccg
      601 gaacgctacc taagctcaac aaacgcagta ttgaatttgc agttcgtttg ggtctggcat
      661 gcaaatccac tatccgttct gaaatgcatt ttgcccggaa gaattatttc tacgcagact
      721 taccaaaagg atatcagatc acacaggaca atacccccat ttgtaccgag ggtgaaatca
      781 taatcaagga cgatcaggga aatgacaaac gcattggttt gatacggata cacatcgaag
      841 aagatgctgg taaaagcatt cacgatgttg atccgttcga cacgttagtt gatctcaacc
      901 gggcaggcgt tcccttattg gaaattgttt cctctcccga tatgcgcaca ggaaaggaag
      961 cttataatta ccttacagaa gtaagaaaac ttgttcgtta ccttgacata tgcgatggca
     1021 atatggaaga aggttcgatg cggtgtgatg cgaatatatc tgtaagaaaa aaaggaagtc
     1081 aagaatttgg aacacggact gagactaaaa acatcaattc aattcgcaat gttcagcgcg
     1141 ctattgaatt cgaaataaaa cgtcagatcg atgttctgga agcaggcggt acagtcgtac
     1201 aggagactcg gtcatttgat gcgccaacag gcggaaccat aagtatgcga agcaaggaaa
     1261 tggcgcatga ctatcgctat ttccctgagc cagatcttcc accggtgaaa gtttctcaag
     1321 agtatatcgc aaatgtaaag gctgctcttc ctcctcttcc tgcagaactg tttttacgat
     1381 acacgaaaga acttggattg cctgaatacg acgcttatca attgacagat cacaaaccta
     1441 ttgcattgtt ttttgaagaa ctgatcaagc acaccggaaa ttataaatcg gccgcaaact
     1501 ggataatggg agaagttcgg tcgtggctca atgagaacgg tttggaaatt gaagacttcc
     1561 ctcttcctgc atcacgaatt gctgaaatca ttgcactgat tgatgcatca aaagttagtc
     1621 ataccgttgc ttcacaaact attttcccag ctctgattgc aaaccctgaa aaaacagcat
     1681 tgcgaattgc cgaagagaaa aaccttattc aggaaagtga ttcaggtgca ttgattggat
     1741 ttatccatga ggcgataaaa aatattggcc cggataaact tgctgaatac aaaggtggaa
     1801 aaaaaggact ggtaggactt tacatggggg aggttatgaa actttccaaa ggaaaagcag
     1861 acccgaaaag caccaatcaa ttattggtaa aaactctcga cgaaacaaca ttatgaaagt
     1921 gaaacaagta tttaccttac tgatttttgc cacagtaatg atctcctgcg gtggcggtgg
     1981 taatcagatt tcaggatcat ttacaaatgg atcaggaaaa acgatttatc tggaacgctt
     2041 tgaaaaatca atgcccgttg ttgttgattc ggcaaagctt gctgacgacg gaagtttcag
     2101 ccttacattt cccaataaaa cagaactgta tcgtttgcgc atacaaccta acgattttgc
     2161 ggttttgatt cttgacagca ccgacaagcc tgagataaaa ggagatgcaa atcagattta
     2221 tgctacttat accatttcag gtgcacgtaa cagtgagctg attcaatctt tctttaagcg
     2281 taccaacgaa tacatgatag agcgtgaaac gcttcgacaa aaattaatgg cggttgccat
     2341 tgatg
//
LOCUS       DAOMYG010000477         2338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_17532_length_2338_cov_6.747262, whole genome shotgun sequence.
ACCESSION   DAOMYG010000477 DAOMYG010000000
VERSION     DAOMYG010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17532_length_2338_cov_6.747262"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1489)
                     /gene="dnaA"
                     /locus_tag="PK610_12735"
     CDS             complement(59..1489)
                     /gene="dnaA"
                     /locus_tag="PK610_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRJ36852.1"
                     /translation="MKKNHETVWKNCLDIIRDNVSLQSFKTWFEPIKPLKLENNVLTI
                     QVPSQFFYEWLEEHYITLLKKTIKKELGTEGRLEYSIVMENTYGSQSPYTVKIPTTNA
                     KALKNPPLSMPLDISSNPIKNPFIIPGLRKVNVDSNLNPNYSFENFVEGDCNRLARSA
                     GYAVANKPGGTAFNPLLIYGGVGLGKTHLAHAIGISIKNQYPNKTVLYVSSEKFTHQF
                     IDAVKNNTTNDFIHFYQMMDVLIIDDVQFFAGKERTQDVFFHIFNHLHQSGKQIVLTS
                     DKPPVEMQGMEQRLLSRFKWGLSADLQAPSLETRIAILEKKMYADGIELPREVVEYLA
                     YSITTNIRELEGALISLIAQSSLNKKAITLDLAKQMIDKFVKNTAREVSIDYIQKVVC
                     DYFDLPIELLKSKTRKREVVQARQIAMFFAKKMTKSSLANIGMHCGGKDHATVLHACR
                     TVNNLVETDKQFRGYIEELEKKLSIQ"
     gene            complement(1660..>2338)
                     /locus_tag="PK610_12740"
     CDS             complement(1660..>2338)
                     /locus_tag="PK610_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HRJ36853.1"
                     /translation="GDIFQGTPYFNFFGGEPELRLMSDMGYEAATMGNHDFDNGLEGF
                     LKVLHHAQFPFLCSNYDFSGTLLNKHTFDHKIIEKGGLKIGLFGIGIELKGLVTEKNY
                     GKTVWLDPIEIAKDRSKMLKQAGCNLIICISHLGYQYANEKVSDIKLAKESTMIDIIL
                     GGHTHTFMEEAAIVTNKTGKPVLVNQAGWGGLRLGRIDISFNKSRSGGTSIGEISYLQ
                     SSNRNVI"
BASE COUNT          622 a          480 c          503 g          733 t
ORIGIN      
        1 caaaaatagg aaacaaaaaa gaccccgatt ggggtctttt ctgatttatt tgttcaaatt
       61 attgaatgct gagttttttc tcgagttctt caatgtaacc acggaactgc ttgtcggttt
      121 caacaaggtt gtttacggtt ctgcaggcat gaagtacagt agcgtgatct ttgccaccgc
      181 agtgcatacc aatgtttgcc aatgaagatt tggtcatctt ctttgcaaag aacattgcga
      241 tctgacgagc ctgcaccact tcacgtttac gtgttttgga tttaagcagt tcgataggaa
      301 gatcgaaata atcacaaacg actttttgaa tatagtcgat ggaaacttcg cgtgcagtat
      361 tcttcacgaa tttgtcgatc atctgctttg caagatcaag cgtgatggct ttcttgttca
      421 gtgatgactg agcgataagt gaaatcaatg caccttccag ttcgcggata ttggtagtga
      481 tggagtaggc aaggtattct acaacttcgc gaggaagttc gataccatca gcgtacattt
      541 tcttttcaag aatggcaata cgggtctcga ggcttggcgc ctgaagatct gctgataatc
      601 cccatttgaa acgcgagagc aaacgctgct ccatcccctg catttccaca ggtggtttat
      661 cagatgtaag aacaatctgc tttccgctct gatgaagatg attgaagatg tggaagaaca
      721 catcctgcgt acgttcctta ccggcaaaga attgaacatc gtcaatgatc aatacatcca
      781 tcatctgata gaagtgaatg aaatcattgg ttgtgttgtt ctttacagca tcaatgaact
      841 gatgggtgaa tttttcagaa gaaacataca ataccgtttt attcgggtat tggttcttga
      901 tcgaaattcc gattgcatga gccaggtgtg tcttacccaa gccaactccc ccataaatga
      961 gaagcggatt gaatgcggtt ccaccgggct tgttggctac ggcataccct gctgaacgtg
     1021 caaggcggtt gcagtctcct tctacaaaat tttcaaacga gtaattgggg ttaagattag
     1081 aatccacatt aactttcctc aaacccggaa tgatgaacgg gttcttgatg ggattggaac
     1141 taatgtccaa tggcatcgaa aggggcggat tcttcaatgc ttttgcgttc gtagtcggaa
     1201 tcttgacagt gtagggcgat tgtgatccat acgtgttttc catcacgatg gagtactcca
     1261 gacgtccttc agtacctaat tccttcttga tcgttttttt gagaagcgtt atataatgct
     1321 cctctaacca ctcgtagaaa aattgagagg ggacctggat cgtaagtaca ttgttttcaa
     1381 gtttaagtgg cttgataggc tcaaaccacg tcttgaaact ttgcaggctc acgttgtcgc
     1441 ggatgatatc cagacaattc ttccagacgg tctcgtgatt tttcttcatt cgttgtgttg
     1501 gtattatttg gtttgtttgt ttgaccgcgt ccggtcattg gggttgcgaa tatagaatat
     1561 ttgatactcc cgatgaccgt acaaagcaaa tatttacata aaaaagttta aaaaaaaatg
     1621 aaggttagct tgattttcta aaaagttttt tttgggcgat cagataacat ttctgttact
     1681 actctgaaga tacgaaattt ctcctataga agttcctccc gatcgtgatt tattaaagga
     1741 aatatcgata cgtcctaatc gcaagcctcc ccaacctgcc tgattaacca gtacaggttt
     1801 tcctgtttta tttgtaacaa ttgctgcttc ttccataaat gtatgcgtat gtcctcctaa
     1861 tatgatatcg atcatggtgc tttcctttgc aagttttatg tctgatactt tttcgttagc
     1921 atactgatac ccaaggtgcg aaatacagat gatgaggttg catcctgcct gctttagcat
     1981 ttttgagcgg tccttagcta tttcaatagg gtcaagccag acggtttttc cataattttt
     2041 ttctgtaacc aatcccttta attctatacc aatcccgaac agacctattt tcaggcctcc
     2101 cttttcaatt atcttatgat cgaaggtgtg cttgttcagt aaagtgcctg aaaagtcgta
     2161 attcgagcat aaaaagggga attgggcatg gtgcaggact ttcagaaagc cttccagccc
     2221 gttgtcgaaa tcgtggttgc ccatggttgc ggcttcgtag cccatgtccg acatcaaccg
     2281 aagttccggt tcacctccaa agaaattaaa gtagggtgtg ccctgaaaaa tatccccg
//
LOCUS       DAOMYG010000478         2332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_17585_length_2332_cov_9.914800, whole genome shotgun sequence.
ACCESSION   DAOMYG010000478 DAOMYG010000000
VERSION     DAOMYG010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17585_length_2332_cov_9.914800"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1683)
                     /locus_tag="PK610_12745"
     CDS             complement(139..1683)
                     /locus_tag="PK610_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRJ36854.1"
                     /translation="MNIELIIDAGPTEVVIALLQDKRLVEIHREKGNNSWSVGDVYLG
                     KVKRVVPGLNASFVNVGYEKDAFLHYLDLGPQFSSLAKFTKRSLTRKQSTSHLDQFEM
                     EKDIDKEGKISQVVSANQNILVQIAKEPISSKGPRLSSEVTLAGRYIVLVPFSNKISI
                     SSKIKDEDERKRLRGIIDSVRPKNFGVIIRTVAQNKNLSELDADLNALVAKWDQLYKA
                     LKDAEPPKKILGELDRTAALLRDLLNEKFNNIHVNDPTLYEEIRNYVRKISPGSEGIV
                     KLYSGPNNIFDHFGIHRQIKAAFGKQVTLQSGGYLIIEHTEAMHVIDVNSGNRKSTDQ
                     EANALQVNMEAADEIARLLRLRDMGGIVCVDFIDMGDRENQKKLFDHLKACMKLDRAK
                     HNVLPPSKFGVVEITRQRVRPETSISTTEKCPCCMGSGEVGATLLITDDIQNQLRYLL
                     ADKKMSGISLAVHPFLYAHFTKGIFSQRWKWFLKYKKWIRIKGVTSHHLLEYHFIDKY
                     GEEVLD"
     gene            complement(1881..>2332)
                     /locus_tag="PK610_12750"
     CDS             complement(1881..>2332)
                     /locus_tag="PK610_12750"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ36855.1"
                     /translation="IILLVAFLLIGFLMSAPRTLPDKSFEGMNPEQAAVVLALNYMGG
                     GNGPMKGIKMLDKITELHPENTIAVGRLAEFSIQTGQYEKAVGRFEALVAATAGREQV
                     EALMGLSDAAFLAGDTTKCLDALQRVLAMSEDSLLLQSVQKKINELR"
BASE COUNT          621 a          517 c          507 g          687 t
ORIGIN      
        1 tttgccttgc ccagccgatc ctgcggcaac tggtgaaaat gctgggaacg agcaaccagg
       61 acgtgtcacg ggtattcttc cctatcgaac gcttggtatt caaatcagtg atgccaaaga
      121 tgtctttctg agaaaaagtc agtccagcac ctcttcccca tacttatcga tgaaatgata
      181 ttcgagtaga tggtgagagg taactccttt gatacggatc cattttttgt atttcagaaa
      241 ccatttccag cgctgggaaa aaattccctt tgtaaaatgc gcataaagaa aggggtgaac
      301 agccagacta atgcccgaca ttttcttatc tgccagaaga tagcgcaatt ggttttggat
      361 atcatcggta ataagtaaag tagcaccaac ttcaccagag cccatacaac aaggacattt
      421 ttcggttgtg gaaatggatg tttcaggacg aacacgctga cgtgtaattt ccaccactcc
      481 gaatttactt ggaggaagta cattgtgttt tgcacgatcc agtttcatgc atgctttgag
      541 gtgatcgaaa agttttttct ggttttcgcg atcacccata tcaatgaaat cgacacaaac
      601 gattccaccc atatcacgca gacgcagcaa acgtgcaatt tcatctgcag cttccatgtt
      661 tacctgaagt gcattagcct cctgatcggt gcttttgcgg ttacctgaat tcacatcgat
      721 aacgtgcatt gcttcggtat gttcaataat caggtatcca cccgactgaa gtgttacctg
      781 ttttccgaat gcagctttga tttgacgatg aattccgaaa tgatcaaaaa tattgtttgg
      841 tccggaatac aacttaacga taccctcgct gccgggagaa attttgcgga cataattcct
      901 tatttcttcg tagagcgttg gatcgtttac gtgaatattg ttgaatttct cattgagaag
      961 atcgcgtaac aatgctgcag tacgatcgag ctcaccgaga attttctttg ggggttctgc
     1021 atctttcaat gccttgtaca gctgatccca ttttgcaaca agtgcattga gatccgcgtc
     1081 cagttcactg aggtttttat tttgagcaac tgtccggata attactccaa aattcttagg
     1141 tcggacagaa tcaataattc cgcgaagtcg tttgcgttcg tcttcgtctt tgatcttgga
     1201 agagatagaa attttattgc tgaacggaac aagaacgatg taacgtccgg caagtgtaac
     1261 ttcagaactt aagcgtggac ctttggatga aataggttct ttggcaattt gcaccaggat
     1321 attctggttt gcggaaacaa cctgactgat cttaccttct ttatcgatat ccttttccat
     1381 ttcgaactga tcgagatggg aagtgctttg tttgcgggta agggagcgct ttgtgaattt
     1441 ggcaagcgaa gaaaattgcg gaccaagatc gaggtaatga aggaatgcat ctttctcgta
     1501 tcctacgttt acgaacgagg cattcagtcc cgggacgact cgtttgacct ttcccaggta
     1561 tacatcgcca accgaccagg agttattgcc tttttctctg tgaatttcta caagacgttt
     1621 gtcttgaaga agagcaataa ccacctcagt gggaccagca tcgatgatga gctcgatgtt
     1681 caagcagtta cgtgttgacc gacaaatctt ttactagcgc caaaaggcac agataaactg
     1741 tgccccggct aaaagattga tcaaagaagg attacttctt ctttttatga cggttcttgc
     1801 gaagacgctt cttccgcttg tgggtagaca tcttgtgacg ctttcttttt ttaccgcaag
     1861 gcataattgt aaatattaag ttatcgtaat tcgtttattt ttttctgaac ggactgcaaa
     1921 agtaatgaat cctccgacat agccaagacc ctttgcaagg catccaaaca tttcgttgta
     1981 tcaccagcta aaaatgctgc gtcggataag cccattaatg cctcaacctg ttctctacca
     2041 gcagttgccg ctacaagtgc ttcgaatcgt cccactgctt tttcatactg acctgtttga
     2101 atggaaaatt cggccaggcg tccgacagcg atggtattct ccgggtggag ttcggtaatc
     2161 ttgtcgagca ttttgatacc cttcattggg ccattgccac ctcccatata attgagcgcc
     2221 agcacaacag cagcctgttc gggattcatt ccttcaaagc ttttgtcggg cagtgtccgt
     2281 ggagccgaca tgagaaaacc tattaaaaga aaagccacca gaaggataat cc
//
LOCUS       DAOMYG010000479         2327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_17638_length_2327_cov_9.077465, whole genome shotgun sequence.
ACCESSION   DAOMYG010000479 DAOMYG010000000
VERSION     DAOMYG010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17638_length_2327_cov_9.077465"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1324
                     /locus_tag="PK610_12755"
     CDS             113..1324
                     /locus_tag="PK610_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HRJ36856.1"
                     /translation="MRPVYFIFLTLLIDSLGIGIIFPVMATILSEITGESINDAAAEG
                     GLLMAIYAGMQFVFAPVLGGLSDRYGRRPVLLLSLLGLGLDYFILCFADSYTWLIVGR
                     AIAGLCGGSFTTAYAYIADVTAPEKRAQAFGLMGAAFGLGFIVGPFVGGLFSDLGSRA
                     PFVAAAVLSLLNFLYGLFVLPESLAKENRRPFDIRRANPFGAFLQLKKMRGVRLLVAC
                     MFLLYLAGQVMPAIWTFYTKYMFDWSDKAVGYSLAFVGVMVSIVQGGLIGWSQKAFGA
                     VRSIYIGLGLYITGLLLFAFADQEWMMYAWTFIYALGGVGPPSMQGIISSQVSANEQG
                     ELQGMLTSLMSLATVVSPLLMTWLFSYFTSPAAPVHFPGVSFLASGILIGFAILLFVF
                     RQTKSTVQEAK"
     gene            1502..>2327
                     /locus_tag="PK610_12760"
     CDS             1502..>2327
                     /locus_tag="PK610_12760"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRJ36857.1"
                     /translation="MRILIFFLFLPLAAFSKKKELTFSTFKGDAIIKVFVLENPRAHA
                     TYVMRLHESGTAELNKYTNGKKPAFRQVTGKFRDKRRSISFQWKNTSEINFFPSKLYK
                     SKNGLHYSMVDAIRGEEADIEANEDSRFRKVCYIDPVTNTLIRDKDFAAQLEVDELAL
                     AITEGAESKEEKIAAIVQYMCGQFKYGEGEQKDLRKILAQQEPRLVCMGYAEVFFQLA
                     VANGIEVRKETGNVPNHVALALATGGEGHMWNVVRLNGEDRIVDVCWIDGLNGEDPL"
BASE COUNT          583 a          442 c          548 g          754 t
ORIGIN      
        1 gatgacaaag gtttttaaga atccaataaa tagtatttga gtttttccat tcgctataaa
       61 gttgctatga tctaaaacaa ttctgtaata ttgcgctttc actttgtcat ttatgcgtcc
      121 cgtttatttt atttttctta cactgctaat cgatagcctt ggaattggaa tcatttttcc
      181 ggttatggca accatactct ccgaaatcac cggtgaatcc attaacgatg cagcagcaga
      241 aggaggattg cttatggcaa tttatgcggg aatgcagttt gtttttgcgc ctgttcttgg
      301 cggattaagt gatcgctatg gacgccgacc tgtgttgttg ctttccttac ttggcttagg
      361 actcgattat tttattctct gtttcgcaga ctcctacaca tggctgattg ttggtcgtgc
      421 cattgccggt ttatgtggtg gaagttttac cactgcttat gcctatatcg ccgatgttac
      481 tgcacctgaa aagcgtgctc aggcttttgg tttgatggga gctgcgtttg gacttgggtt
      541 tattgttgga ccatttgtag gtggtttgtt cagtgatctc ggttcaagag ccccgtttgt
      601 agcagcagca gttttgtcat tgctcaattt tctgtacgga ctatttgttc ttcctgaatc
      661 attggcaaaa gaaaaccgta ggccattcga tatccgtcgc gctaatccat ttggtgcgtt
      721 tttgcagttg aagaaaatga gaggtgtgcg tttgcttgtt gcctgtatgt ttctccttta
      781 tcttgccgga caagtaatgc ctgctatctg gacattttat accaaataca tgtttgattg
      841 gagtgataag gcagtgggct attcactcgc atttgttggt gttatggttt ccattgtaca
      901 aggaggattg atcggctggt cgcaaaaagc atttggagca gtaagatcca tttatattgg
      961 actcggatta tacatcacgg gtctgttgtt gtttgctttt gcagatcagg agtggatgat
     1021 gtatgcctgg acatttatat atgctttagg tggagttggt cctccatcca tgcaggggat
     1081 catcagttcg caggtatccg caaacgaaca aggcgaattg cagggaatgc tcacaagttt
     1141 gatgagtctt gcaacagtgg tttctccttt gctaatgacc tggttgtttt cctatttcac
     1201 atccccggct gctccggtcc actttccagg ggtttcgttt ttggcatccg gtatcctcat
     1261 tggttttgcc atccttctat ttgttttcag gcaaaccaaa tccacagttc aggaggccaa
     1321 ataaagtatt ttatcctgct ttaagtgcca aatctccttt tgtgaaataa gggaacttaa
     1381 gaatttttcg aaataattgt aacctgtttc tcttttctgc gttctacttc ccaaaaccaa
     1441 gtcatgggaa tacgctactc ttttgatttt ttatctggta caatttaacg gagggagttt
     1501 tatgcgtatt cttatttttt tcctgttttt acctcttgct gctttttcaa aaaagaaaga
     1561 gcttacgttc tcaacgttta agggagatgc aataatcaaa gtatttgttc tggagaatcc
     1621 gcgtgcacat gcaacctatg tgatgcgcct gcatgagagt ggtacagcgg aactcaacaa
     1681 atataccaac ggcaaaaaac cggcattccg tcaggttacc ggaaaattcc gcgataagcg
     1741 ccggtctatt tcatttcaat ggaagaatac ttctgaaatc aattttttcc cttcgaaact
     1801 ctataaaagt aaaaacggat tgcactacag catggtagat gctattcggg gtgaagaagc
     1861 cgatatagaa gcgaatgagg atagccgttt tcgtaaggtg tgctatatcg atccggtaac
     1921 aaatacttta atacgcgata aagattttgc cgctcagctg gaggtggatg aacttgcgtt
     1981 ggccattacc gaaggagctg aatcgaaaga agaaaaaata gcagccattg ttcaatatat
     2041 gtgcggacaa ttcaaatacg gtgaaggtga acaaaaggat ttgcgtaaaa ttttggcgca
     2101 gcaggaaccc cgtctcgttt gtatgggata tgcagaagtg ttttttcagt tggcagttgc
     2161 taatggaatt gaagtgagaa aagaaacagg taacgttccc aatcatgtcg ccctggcttt
     2221 ggccacagga ggagagggtc acatgtggaa tgtagtacga ctgaacggag aggatcggat
     2281 agtggatgtg tgctggatcg atggactcaa tggagaggat cctctcc
//
LOCUS       DAOMYG010000480         2308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_17836_length_2308_cov_8.783400, whole genome shotgun sequence.
ACCESSION   DAOMYG010000480 DAOMYG010000000
VERSION     DAOMYG010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17836_length_2308_cov_8.783400"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PK610_12765"
     CDS             <1..197
                     /locus_tag="PK610_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36858.1"
                     /translation="PFELQSDGTRTYFVYATNRKRKHVTMWISFIGSIDNSEFFTQYF
                     PPQEVFGINCRHIYYFKRKV"
     gene            329..523
                     /locus_tag="PK610_12770"
     CDS             329..523
                     /locus_tag="PK610_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36859.1"
                     /translation="MKKRIKPVESFVSVVKLERTRFEDLDDRLLVFHLFRLGQRYVEA
                     EMEAIYFGGPVGARVFYSLN"
     gene            530..733
                     /locus_tag="PK610_12775"
     CDS             530..733
                     /locus_tag="PK610_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36860.1"
                     /translation="MAGYFVARKRRMVLEVDELVFVPDCRDEQEARRKVLTGEGVPLK
                     GSEKVSGRRWRDRILDAGEAIEA"
     gene            801..1271
                     /locus_tag="PK610_12780"
     CDS             801..1271
                     /locus_tag="PK610_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36861.1"
                     /translation="MKILVASFISIALLSCGEQQKENNSTGSVPLQDSTTIEAPLRED
                     YSDALVMNGNGIVGMFRDFSVNKQWNEMMRFTSGTIVARYGEEKVRSFYAGLDLTQPL
                     IFESVRDEGEVKVLNYRVTGKADEFRKLRVVEENDTVRVWPFDFEKGLFFSGEK"
     gene            complement(1397..2011)
                     /locus_tag="PK610_12785"
     CDS             complement(1397..2011)
                     /locus_tag="PK610_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36862.1"
                     /translation="MATLSIQIKGPAVSLRSSSLSEDTWERCREKIGLKGSGGWYGLL
                     EDDFFLGMDAVTRSGKAILHWQDLDDDLYLLGAIPARRTIMRIKHSAKRKSFHKTLNQ
                     LLEHGMLFPLFHNSIRSIAFDPDNEHNVAIAESRVGPLFTFTWDVPDLELFNPEALEF
                     CWVKVNLYGKREFLLFTDILYKKMPAKEIKTGKTVVKSWELIRK"
BASE COUNT          603 a          433 c          537 g          735 t
ORIGIN      
        1 tcccatttga gttgcaatcc gatggaacaa gaacatattt tgtttatgca acaaacagaa
       61 aaagaaagca tgttacaatg tggatttcat ttataggctc aatagataat tcagaatttt
      121 ttacccagta ctttccacca caggaggtgt ttggcataaa ttgccggcat atttattatt
      181 ttaaaaggaa ggtttaacca atcgttttga tcaagcgcct cacttgtggt taagtattca
      241 gatcctcttt tcactttttc tcttcaactt ctcccacaac gcaaattcac tgcgttctgt
      301 ccgtttttct ttgaataaat ttctgatcat gaaaaagcgt attaaaccgg tggagtcttt
      361 tgtttccgtt gttaaacttg aacgcacgcg ttttgaagat ttggatgatc gtttattggt
      421 ttttcatttg ttcaggctgg gtcagcgtta cgtagaggcc gaaatggaag ccatttattt
      481 tggtggtccg gttggtgcga gggtgtttta ttcattgaat tgaaaaagta tggcaggtta
      541 ttttgtagca cgaaagcgtc gtatggttct tgaagtggat gaattggtct ttgttccgga
      601 ttgcagggat gaacaagagg cacggagaaa ggtcctgacc ggcgagggtg ttcccttaaa
      661 aggttcggaa aaggtatcag ggaggcgatg gcgcgaccgt attttggatg caggagaagc
      721 aattgaagcc tgatttttac ggaattagtg ggctgacagg aatcgttgtt gaaaacccgg
      781 aatttgtgta ttttcccttt atgaaaattt tagtcgcatc ttttatttct atcgccttgc
      841 tttcttgtgg cgaacaacaa aaggaaaaca attcaaccgg atctgttcca ctacaggatt
      901 ctaccacgat tgaggctccg ctaagggagg attattcgga tgcgcttgtg atgaatggga
      961 atgggattgt gggcatgttc cgggattttt ctgtcaataa acaatggaat gagatgatgc
     1021 gctttacatc cggaaccatt gtagcgcgtt acggagaaga gaaagtacgt tccttttatg
     1081 caggactgga tctcactcag ccattgattt ttgaaagtgt aagagacgaa ggtgaagtaa
     1141 aagtgttgaa ttaccgtgtt acaggtaagg cagatgagtt cagaaaacta agagtagtag
     1201 aagagaacga tactgttcgt gtatggcctt ttgattttga aaagggattg tttttttctg
     1261 gtgaaaagtg aatagtgaaa ggtgatcccg acgctccccg acgctaccgg tcggggcagg
     1321 atcggtcggg tcaggccagt gaaaggtgaa tagtgatttc aacgattcag tatgctaact
     1381 gtcggaacgg cttgttttat ttccttatca attcccagct tttaacaact gtttttccgg
     1441 tcttaatttc ttttgccggc atttttttgt aaaggatatc cgtgaaaagg agaaactcac
     1501 gtttgccgta aagatttact ttcacccagc agaattcgag cgcttcggga ttgaacaatt
     1561 caagatcggg tacatcccag gtaaaggtaa atagtggacc taccctgctt tctgctatgg
     1621 caacattgtg ttcattatcc ggatcgaatg caatggagcg gatgctgtta tgaaacagtg
     1681 gaaataacat tccatgctcc agcaattggt ttagcgtttt atgaaaactt ttgcgttttg
     1741 cgctgtgttt gatgcgcatg atggttcttc gggccggaat ggctcccaat aaataaagat
     1801 catcatccag atcctgccaa tgcagaatcg cttttcccga tcgtgtaacg gcatccatcc
     1861 ccaaaaagaa atcgtcttcg agtaaaccgt accacccacc gctgcctttc aatccgattt
     1921 tttccctgca cctctcccag gtgtcttccg aaagagaaga tgaacgtaag gatactgccg
     1981 gtcctttgat ctgaatactc agcgtggcca tcagctgcta aagtaatacc ctggtccgct
     2041 ggcaaaccca aattgtttat taatcgcctc acttgttcaa aattgattcg ccggtgcatt
     2101 catgcagaat cgctaatgtt ctttggtttt ctggcatcgg tggagactga ttttaatcat
     2161 ttcttatcgc aaataacgtc ccgattttgc actttttcta cctttttttc gggtgttttg
     2221 tccgaactaa gcacatggat gtaagacttt tgatatcgtt ggtgtagtgt ttttttgata
     2281 tgttaaaaat ttgattctga atagtatt
//
LOCUS       DAOMYG010000481         2291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_18038_length_2291_cov_7.618515, whole genome shotgun sequence.
ACCESSION   DAOMYG010000481 DAOMYG010000000
VERSION     DAOMYG010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18038_length_2291_cov_7.618515"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PK610_12790"
     CDS             complement(<1..319)
                     /locus_tag="PK610_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ36863.1"
                     /translation="MNFSWHIAKRMLKNEKGEDRFARPVLRIAVWSIALGIAVMILSL
                     SVVTGFQEEIRNKVIGFGAHIQITAFNANPSLESDPVLISQDFYPSLNDEPGVKNIQV
                     YAYK"
     gene            344..1600
                     /locus_tag="PK610_12795"
     CDS             344..1600
                     /locus_tag="PK610_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRJ36864.1"
                     /translation="MKRTLLPTACLLFSALFACSSGRTSQAAPSEHALSSDTPSVKKE
                     QIAEKPIMGAERMDSVLPLISGKKVAVVANQTSLVNGVHLVDTLIASGVDIQKVFALE
                     HGFRGEVQAGEHIKSGKDPKTGVPIVSLYGNNKKPKAEVLQDVDVIIFDIQDVGARFY
                     TYISSLHYIMEAAAENGKTVLVLDRPNPNGFYVDGPVLNMEHKSFVGMHPVPIVHGMT
                     VAEYAQMINGEAWLAGGVQCDLKVVLCSNYDHSVLYELPVKPSPNLVNLAAIYLYPSL
                     CLFEGTFVSVGRGTDFPFQVIGMPDYSPGTFRFTPRSVAAAKKPPHMDVECNGYDLRD
                     SANNVLKTRCINLNWLLTMYANCTDKKNFFENDGMFTLLAGGKVLREQIEKGMSEAEI
                     RKSWEKELNSFREIRKKYLLYKDFEP"
     gene            1703..>2291
                     /locus_tag="PK610_12800"
     CDS             1703..>2291
                     /locus_tag="PK610_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016478528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRJ36865.1"
                     /translation="MNISVSWLRHYLKTDLATNELADLLTDIGLEVESVEKVESVKGG
                     LEGMVVGEILTCDKHPDADRLKITTVNVGEAEPLQIVCGAPNAAAGQKVIVALNGAKL
                     FPSEGDPFTIKKSKIRGVESNGMICAEDEIGLGKGHDGIMVLPPEVEVGLPAAKYFQL
                     ESDEVISIGLTPNRVDAASHYGVARDLYAAIRFRGD"
BASE COUNT          619 a          460 c          563 g          649 t
ORIGIN      
        1 gcttgtaggc atatacctga atatttttta cacccggttc atcgttgagc gaagggtaaa
       61 aatcctgcga tatcaatacc ggatctgatt ccagcgatgg gttggcatta aaggccgtta
      121 tttgaatatg agcaccgaaa ccaataactt tattgcggat ctcctcctgg aaaccggtaa
      181 ccaccgataa agataaaatc atcacggcaa ttccaagagc gatgctccaa acagcaatgc
      241 gtaaaacggg acgggcaaag cgatcttcac ctttttcgtt ttttagcatg cgttttgcaa
      301 tatgccaact gaaattcata ctcaacgaaa gtacagaatc tgaatgaaac gaacacttct
      361 tccaactgcc tgtctattgt tcagcgcact attcgcttgt tcttccggca gaacatccca
      421 ggctgcgcct tctgaacatg ccctttcaag tgatacacct tccgtgaaaa aggagcagat
      481 tgcagaaaaa ccaatcatgg gtgcagagcg aatggatagt gtattgcctc tcatttcggg
      541 aaagaaagta gcagttgtag cgaatcagac ttcactcgtg aatggtgtgc atttggttga
      601 tacgttaatt gcttcgggtg ttgatatcca aaaagttttt gcgctcgaac acggatttcg
      661 cggagaagtg caggcaggtg agcatatcaa gagtggtaaa gatcctaaaa caggagtacc
      721 catcgtttcc ctgtatggca ataataaaaa accaaaggca gaagttttgc aggatgtgga
      781 tgtcattatc ttcgatattc aggatgtggg agcgcgtttt tacacgtata tttcttcgtt
      841 gcattacatt atggaggctg cagctgaaaa tggcaaaact gttcttgtac ttgatcggcc
      901 caacccaaat ggattttatg tggacggacc ggtgttgaac atggagcata agtcgtttgt
      961 aggaatgcat cctgttccca ttgtgcatgg aatgactgtt gctgaatatg cgcaaatgat
     1021 caacggtgaa gcctggttgg caggtggtgt gcaatgcgat cttaaagttg tattgtgcag
     1081 caattacgat cattccgttc tgtatgaatt gccggtgaaa ccttctccca acctggtgaa
     1141 tctggctgcg atctacctct atccttcgct gtgtttgttt gaggggacat ttgtttcggt
     1201 tggacgcgga actgattttc cttttcaagt gatcggtatg ccggattatt cccccggaac
     1261 attccgtttt acaccgcgat ctgttgctgc tgccaaaaag cctccgcata tggatgtgga
     1321 atgtaatggt tacgatctgc gcgattcggc aaacaatgtg ctgaaaaccc gatgcatcaa
     1381 cctgaattgg ctgcttacga tgtatgcaaa ctgtacggat aaaaagaatt ttttcgagaa
     1441 cgacggtatg ttcactttgc tcgcgggtgg aaaagtgctg cgggaacaga tcgagaaggg
     1501 gatgagtgag gcagaaattc gtaaaagctg ggaaaaagaa ctgaattcct tcagggaaat
     1561 ccgtaaaaag taccttctgt acaaggattt tgagccttga ttcgtttttc cataggcgat
     1621 ttcgataatt ttaacaatac cgcagtcttt ccctctctca gaggactgaa attgttattt
     1681 ttgccccact gattctcttc acatgaacat ctctgtatcc tggttgcgac actacctcaa
     1741 aaccgatctt gctaccaatg aacttgccga tctgcttacc gatattggtt tggaagtgga
     1801 aagcgtggaa aaagtggaaa gcgtaaaggg tggtttggaa ggaatggttg taggtgagat
     1861 ccttacttgc gataagcatc cggatgcaga tcgtttgaag attacaacag tcaatgttgg
     1921 agaggctgaa cccttgcaga ttgtttgtgg tgctcccaat gcagctgcgg gtcagaaggt
     1981 gatcgttgca cttaacggtg cgaaactttt cccgagtgaa ggtgatccgt tcaccatcaa
     2041 aaaaagcaaa atacgcgggg ttgaatccaa tggaatgatc tgtgcggaag atgaaattgg
     2101 actggggaaa ggtcacgacg gaattatggt tcttcctcca gaagttgagg ttggacttcc
     2161 tgctgcaaag tattttcaat tggaaagtga tgaagtaatt tcaattggat taacacctaa
     2221 ccgtgttgat gctgcttcgc attatggtgt ggctcgcgat ttatatgctg ctatccgctt
     2281 tcgcggtgat a
//
LOCUS       DAOMYG010000482         2289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_18066_length_2289_cov_6.239481, whole genome shotgun sequence.
ACCESSION   DAOMYG010000482 DAOMYG010000000
VERSION     DAOMYG010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18066_length_2289_cov_6.239481"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="PK610_12805"
     CDS             <1..1084
                     /locus_tag="PK610_12805"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRJ36866.1"
                     /translation="TTEELKFPVKLSDATEKLDRLLVRVNGVPLNGPAGIDLRRYNSR
                     SIDMEVAISLLPGTNHVHVSCVNEKGAESLLHEFEVVRGGASKSNKVYVVAIGVGDYS
                     DDQFDLKYPAKDAKDFISRIRSTNDKTADIRSLLLCDKQVTREALDSIRNFIAAAGSQ
                     DVVILFLAGHGVLDVDYNYFFATEDIDFSNPGVRGIAYEQIEKLFALIPAQRKLLIMD
                     TCHSGELDKTELEKEKQVEVEVKNVQFRAVGAGVKQRQMAGGSSTEMMQLLFADVKDQ
                     TGTVVISSSGGAEFSMESDEWKNGLFTYCILSAGSDFSADINHDKRISVSELRQYTYL
                     KVEKLSKGLQKPTTRSDNLFLDFYVW"
     gene            complement(1085..>2289)
                     /locus_tag="PK610_12810"
     CDS             complement(1085..>2289)
                     /locus_tag="PK610_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013072071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRJ36867.1"
                     /translation="ATEFYKYYREDLALMRSMGIRNFRFSLSWPRIFPNGTGKVNSKG
                     VDFYDRLIDQCLELDIAPWVTLYHWDLPLELEKKGGWTNRSIVNWFSEYAELCAKRYG
                     DRVKKWMVLNEPIVFTGAGYFMGVHAPGRRGINPFLKAVHNTTLVQGLTPEIIRHHSK
                     NCEVGTTYSCSHLDAYSENESDVFAKQRADVLLNRLFIEPALGLGYPHQDFKPLERLL
                     DLMGPNDEQLMFGNYDFIGIQNYTREIVKASWIIPYLKARLVPASKRNVPHTQMNWEI
                     YPEAIYTMLKKFGAYPNVKKIYITENGAAFQDQLVNGRVHDLNRTLFLQSYIEQVLKA
                     KNEGINVAGYFIWSFTDNFEWAEGYTPKFGIVHVDMKTQKRTIKDSGYWYRSFLRGEP
                     VPQLAVTA"
BASE COUNT          656 a          437 c          500 g          696 t
ORIGIN      
        1 aaccaccgaa gaattgaaat ttcccgttaa gctaagtgat gcaacagaaa aacttgatcg
       61 tttgttggtg cgtgttaacg gcgttccgct taatggtcct gcaggaatcg atcttcgtcg
      121 ttataactcg cgttccatcg atatggaagt tgcaatttct ttattgccgg gaacgaacca
      181 tgtacatgtt tcctgcgtga atgaaaaagg agcagaatcg ctgttgcatg agtttgaagt
      241 agtacgtggc ggcgcttcaa aatcgaacaa agtttatgtt gttgccattg gagttggtga
      301 ttatagcgat gatcagttcg atctgaaata tccggcaaaa gatgcaaagg atttcatttc
      361 aaggattcgc tctaccaacg ataaaaccgc tgatatacgt tcgctgttgt tatgtgataa
      421 acaagtaacc agagaagctt tggatagcat tcgaaatttt attgctgctg ccggttcaca
      481 ggatgtggtg attttgtttc ttgccggtca tggtgttttg gatgttgatt acaattattt
      541 tttcgcaacc gaagatatcg atttttcaaa tcccggtgta cgtggaattg cctatgagca
      601 gattgaaaag ctatttgcat tgattccggc acaacgaaaa ttattgataa tggatacctg
      661 tcatagtgga gaacttgata aaacggagct ggaaaaagaa aaacaggtag aggtagaagt
      721 taagaatgtt cagtttcgtg cagttggagc aggagtaaag caacgccaaa tggcaggtgg
      781 ttcctccaca gaaatgatgc aattgctgtt tgcagatgtc aaagatcaaa caggtacagt
      841 tgtaatcagt agttccggtg gagcagagtt ttcaatggaa agcgatgaat ggaaaaatgg
      901 attgttcacc tattgtattt tgagtgccgg cagcgatttt tctgcggaca taaatcatga
      961 taaacggatc tcagtctccg aattgcggca gtacacctat ttgaaagtcg aaaaattaag
     1021 taagggccta caaaaaccga caacacgatc ggataatttg tttttggatt tttatgtctg
     1081 gtgatcaggc ggtgacagcc agctgaggaa caggttctcc ccgaaggaag gatcggtacc
     1141 agtatcccga atctttaatg gttcgttttt gtgttttcat atccacatgc acaattccaa
     1201 atttcggcgt atatccttct gcccattcga aattatcggt gaaggaccag atgaaataac
     1261 cggcaacatt tattccttca ttttttgcct tgagcacttg ttcaatatat gattgcaaaa
     1321 acaatgtgcg gttcaaatca tgtactcttc cgtttacaag ttgatcctga aatgctgcgc
     1381 cgttttctgt gatgtaaatt tttttcacat tggggtaagc accaaatttt ttaagcattg
     1441 tataaatagc ttcaggatag atttcccaat tcatttgcgt gtgcggtaca tttcttttac
     1501 tggccggaac taggcgtgct ttcagatatg gaataatcca cgaagccttt acaatttcgc
     1561 gggtatagtt ttgtattcca ataaaatcat aattaccaaa catcaattgt tcgtcgttgg
     1621 gtcccattaa atcaagcaat cgctctaatg gcttaaaatc ctggtgagga tatcccaatc
     1681 ccaaagcagg ctcaatgaat aaccgattca acagcacatc tgctctttgt ttggcaaata
     1741 catcagattc attttcggaa taagcatcca gatgtgagca ggaataggtt gtaccaactt
     1801 cacaattttt tgaatgatgt cggataattt ccggtgttaa accttgcaca agggtagtat
     1861 tatgaacagc ttttaagaag ggattaattc ctcttcttcc cggtgcgtgt acgcccataa
     1921 aatatcctgc ccctgtaaaa acgatgggtt cattcaggac catccacttt tttacacgat
     1981 cgccatatct ttttgcgcaa agttctgcgt attccgaaaa ccaatttaca atggaacggt
     2041 ttgtccaacc tccttttttt tctaattcga gaggaaggtc ccaatggtac agtgtaaccc
     2101 agggagcaat gtcgagttca agacattggt caattagtcg atcgtagaaa tcaacacctt
     2161 ttgaatttac cttaccggtt ccgtttggaa aaatgcgagg ccaggagagt gaaaaacgga
     2221 aattcctgat tcccatactg cgcatcagtg cgaggtcttc cctatagtat ttgtaaaatt
     2281 cggttgcct
//
LOCUS       DAOMYG010000483         2280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_18149_length_2280_cov_9.307865, whole genome shotgun sequence.
ACCESSION   DAOMYG010000483 DAOMYG010000000
VERSION     DAOMYG010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18149_length_2280_cov_9.307865"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..379)
                     /locus_tag="PK610_12815"
     CDS             complement(20..379)
                     /locus_tag="PK610_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36868.1"
                     /translation="MSEILDEPIDAPRESSYPRKRYAPVFVFGPVLLLGLFLKTMHWP
                     GGAFIFFIGLSMMLGHALAILIHFQKNTFITNVGSILLSFVVIYKYIYPFHYQTCLLS
                     GGVAFVSAFFCWLYFRD"
     gene            complement(473..2053)
                     /locus_tag="PK610_12820"
     CDS             complement(473..2053)
                     /locus_tag="PK610_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRJ36869.1"
                     /translation="MKKIRNIGRTLFAVGLLTFVLSLSMSHFVLTEKVAYSQLNTVYT
                     GEGKTAYEILEPQLTQLYGKKYDSRFAFIADYRKIFSDYNAPLKAAGKWEQVIHDDKL
                     VMVVKHSGAGLLTDQLWFRLVFLLSFIGTCIGALMIIFPEYAGKPFGIHNNGIYHSPA
                     SNGGWIGIVVGTFLILFYIVLYFFPQYITNWMLLADPVRKLFSENGEADQWFLYGFIY
                     CLAMVVMGIRMLLKYRGNRYQQVRTFSVMFFQVCFAFMIPELLSALNQPSVDLKHIWP
                     LHYNYFEGERIESMYAFGGIGIAMFWWGVALFVIGVPLFTYLYGKRWYCSWVCGCGGL
                     AETLGDPYRQLSDKSVKAWKIERWMIHSVLVFVVIMTVLVLIAWQTKQDTVLGISSGM
                     LKMIYGFLIGSVFSGVVGTGFYPMMGNRVWCRFGCPLAAYLGLIQRFKSRFRITTNGG
                     QCISCGNCSTYCEMGIDVRWYAQRGQNIVRSSCVGCGVCSSVCPRGVLKLENAPEAGR
                     INEDPIIIGHDGIRLKMD"
     gene            complement(2050..>2280)
                     /locus_tag="PK610_12825"
     CDS             complement(2050..>2280)
                     /locus_tag="PK610_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRJ36870.1"
                     /translation="GVTLLGVRYRHEVCDRWLREEKDIEYVLCHLSDANFDPEFFRHY
                     ENYLVEEYNAKSGKQLQLKKRSWKRIFQGIGK"
BASE COUNT          708 a          547 c          476 g          549 t
ORIGIN      
        1 accaaccttc ctcccccccc taatccctga aataaagcca gcagaaaaag gcagaaacaa
       61 atgcaactcc gccgcttagc aggcaggttt ggtaatgaaa gggataaatg tatttgtaga
      121 tgaccacaaa ggatagcaaa atggatccga cattggtaat aaaggtgttc ttctgaaaat
      181 gaataaggat ggcaagtgcg tgacctaaca tcatggatag tccgatgaaa aaaatgaatg
      241 ctcctcccgg ccaatgcatg gttttaagaa ataatcccag caatagaaca ggaccaaaaa
      301 caaaaacagg tgcataccgt ttacgtggat aggatgattc ccttggggca tcgataggtt
      361 catcgagaat ttcactcatg tactgatttt tgggaatctg tagtgcaaat aacgtaaagc
      421 cttctttttt gtttcagcaa acagatgatc ggcattggca aaacttccgg aatcaatcca
      481 ttttcagacg tataccgtcg tgcccaatga tgatgggatc ttcattgatg cgtcctgctt
      541 ccggcgcatt ttccagtttc aatacgcctc ttggacaaac cgatgaacaa acaccacaac
      601 ccacgcagga tgaacgcaca atattctgac cacgctgagc ataccagcgt acatcaattc
      661 ccatctcgca ataggtggaa caattaccac aggaaataca ttgtccgcca ttggtagtga
      721 tccgaaagcg ggatttgaag cgttggatca atcctagata cgctgccagc ggacaaccaa
      781 agcggcacca tacgcgattt cccatcatgg gataaaatcc tgtccccact actcccgaga
      841 aaacggaacc gatcagaaat ccgtaaatca ttttgagcat tccgcttgaa attccgagaa
      901 cggtatcctg ttttgtctgc caggcgatga gcacaaggac ggtcatgatc accacaaaca
      961 ccaataccga atgaatcatc cagcgttcaa ttttccatgc tttaacggat ttatcggata
     1021 actgacggta aggatcgcca agtgtttctg ccagtccgcc acaaccgcaa acccaggagc
     1081 aataccagcg tttgccatag aggtaggtga agagcggaac accgatcaca aacaaggcaa
     1141 caccccacca gaacatagca attccgattc cgccaaaggc atacatactc tcgatgcgtt
     1201 cgccctcaaa ataattgtaa tgcagaggcc agatgtgttt cagatccacg gaaggctgat
     1261 tgagtgcaga gagcaactct ggaatcataa acgcaaaaca aacctggaaa aacataacgg
     1321 aaaaagtgcg cacctgctga tagcggttac cgcggtactt gagcagcata cgaatgccca
     1381 ttaccaccat ggctaggcaa tagataaatc cgtaaagaaa ccactgatcg gcttctccat
     1441 tttcagaaaa caatttccgg acaggatcgg caagcagcat ccagttggtg atgtattgcg
     1501 gaaagaaata caatacgata taaaaaagga tcagaaaagt tccaacaaca ataccgatcc
     1561 aacctccatt gctggcagga gaatggtaaa ttccattatt gtgaatgccg aacggtttac
     1621 ctgcatactc cggaaaaatg atcatcaagg caccgataca ggttccaata aaacttaaca
     1681 ggaacaccaa tcgaaaccac agctgatcgg taagtaatcc tgcaccggaa tgtttcacca
     1741 ccattaccag tttatcatca tgtatgacct gttcccattt tcctgctgct tttaagggag
     1801 cattgtaatc ggaaaagatt ttgcggtaat cggcaatgaa tgcaaatcgt gaatcgtatt
     1861 tttttccgta caactgcgtc aattgcggct caaggatttc gtaggctgtt tttccttctc
     1921 cggtataaac ggtgttgagt tgggaatagg ctactttttc ggtgagcaca aaatgactca
     1981 tgcttaaact caacacaaag gtgagcaatc caaccgcaaa gagggtcctg ccgatattcc
     2041 ggatcttttt catttcccta tgccttgaaa gatgcgtttc cagctgcgtt ttttcagttg
     2101 gagttgtttt ccgcttttag cattgtattc ttccaccagg taattttcgt agtgacggaa
     2161 aaactcagga tcgaaattgg catcggacaa atgacacaat acatattcga tatccttttc
     2221 ctcccgcaac cagcgatcgc agacttcatg acggtagcgc acacccaaca gggttacacc
//
LOCUS       DAOMYG010000484         2272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_18249_length_2272_cov_8.131258, whole genome shotgun sequence.
ACCESSION   DAOMYG010000484 DAOMYG010000000
VERSION     DAOMYG010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18249_length_2272_cov_8.131258"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1502)
                     /locus_tag="PK610_12830"
     CDS             complement(<1..1502)
                     /locus_tag="PK610_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36871.1"
                     /translation="MNESINQLRKIARNIPVRKVTLPDKENLNHVFLREGSIGIQKVL
                     DSASEVTEELVEVESTPIPFTPKEPLPEENVVLLDKKPEVNHSLVEDDFAKGERQFTE
                     RIKKRNPEEDEVFDPENPKLEIIHEHKQVMRTPMNYYYVLGTVVQDMGSMRITLMVEE
                     LATGRRERTKIDLYERDQVVLYAQQIGELFSQDEQQIEADLLLLTDLLEKHREEELEQ
                     AKASNQNKRHQSVLTAEKQNACVQFLSGKNLMKGIDELIEQAGVVGEESTRRLLFVIA
                     STYKMNTPLHALVQGTSGSGKSHLINSIGQCFPPEDVISMTRVTSKSFYHYTKDELVD
                     KLLLIQDYDGLDEEAQFAFRELQSAGNISSSTTYKDRYGNLMSAVKTVRSHFASLLAT
                     TKAEVYYDNMSRSLISGVDESDEQTRRIITHQNKNLAGLIDHKKEHQAKEFLQNCMRC
                     IRPYQVVNTYADKVYLPFEAKMLRRLNSHYQAFVKQITILHQYQRKKDDQG"
     gene            complement(1499..2170)
                     /locus_tag="PK610_12835"
     CDS             complement(1499..2170)
                     /locus_tag="PK610_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36872.1"
                     /translation="MMIEMQKQKLHAEICERIYQTLYNGIRNVKSEEPRTWLKERGLS
                     CEVTGAGFSSGQITHRKTKEYLKEVESVGFIRANNIVCKNGDTGYLTFARFGVVFPLR
                     DGKGRVVNFYGIRFKLEKEEHSFMNDQGIYPAFPHEMTTQLFITTSIMDAASMIEAKV
                     LDNRDAVICIPDGKILPQHEEAIKRLTQLNSIVWIESPKINATQGLQGRIMPKGYVTK
                     TKKKP"
BASE COUNT          529 a          519 c          434 g          790 t
ORIGIN      
        1 ccttggtcgt cttttttacg ctggtattgg tgaaggatgg taatctgctt aacgaacgcc
       61 tgataatggc tgtttaaacg ccttaacatt ttagcttcaa atggcaggta aactttatcg
      121 gcataagtgt tcaccacctg atatggtcgg atgcaccgca tacaattttg caagaactct
      181 tttgcctgat gctccttttt atgatcgatt aatccggcaa ggtttttatt ttgatgggtg
      241 ataatcctgc gcgtttgttc atcgctttca tccacaccgg aaatcagcga gcgactcatg
      301 ttatcgtaat acacctcggc ttttgttgtt gccaataatg aagcaaaatg gctgcgtacc
      361 gttttaacag cggacattaa attcccgtag cgatccttat aagtggtcga agatgaaata
      421 tttcctgcgc tttgcaattc tcgaaatgcg aattgcgctt cttcatcgag tccatcgtaa
      481 tcctgaatca gcaataattt atccaccaat tcatccttgg tatagtgata aaaggatttt
      541 gaagttacac gggtcatgct tatcacatct tcaggtggga agcattgccc gatggaatta
      601 atcaaatggc tctttcctga acctgaagtt ccttgaacca aagcgtgaag cggtgtattc
      661 attttatagg tggatgcgat cacaaacaaa agccttcggg tactttcttc tcctaccact
      721 ccggcttgtt caatgagttc atcaattcct ttcatcaggt ttttaccact tagaaattgt
      781 acacaagcat tttgcttttc tgctgttaaa acggattgat gccgcttgtt ttggttactg
      841 gccttagcct gttcgagttc ttcttcccta tgtttttcca gtaaatcggt gagcaaaaga
      901 agatcggctt ctatctgttg ctcatcctga gagaataatt ctccaatttg ctgtgcgtat
      961 aaaaccacct gatccctttc atacaaatca atcttggttc tttcccttct tccggttgcg
     1021 agttcctcca ccataagtgt gatgcgcata ctgcccatat cctgaaccac cgttccgaga
     1081 acataataat agttcattgg tgtacgcatg acctgtttat gctcgtggat gatttccagt
     1141 tttggatttt ccggatcaaa gacttcgtct tcttcaggat tacgtttttt aattcgctcg
     1201 gtgaattgcc gttctccttt tgcaaagtca tcttctacca atgagtgatt tacttccggt
     1261 tttttatcca gcaacaccac gttttcttcc ggtaatggtt ccttaggtgt aaatggaatc
     1321 ggtgtagatt ctacttctac cagttcttct gtcacttcgc ttgctgaatc caataccttc
     1381 tgaataccga tacttccttc acgcagaaat acgtgattca ggttttcctt atcgggtaac
     1441 gtcaccttac gcacgggaat attccgcgct atctttcgga gttggttgat tgattcgttc
     1501 atggtttctt ctttgttttt gttacatagc ccttgggcat aattctcccc tgtaaccctt
     1561 gtgttgcatt gatttttggt gattcaatcc atacaatgga gttcagttgt gttaaccgtt
     1621 tgattgcttc ttcatgctgt ggaagtattt ttccatcagg aatacaaatc acggcatcac
     1681 ggttgtccag cacttttgct tctatcatgg atgcagcatc cataatcgat gtagtaatga
     1741 ataactgagt ggtcatttca tgcgggaagg caggatatat tccctgatcg ttcataaagg
     1801 aatgttcttc cttttccagc ttaaaacgga ttccgtaaaa attgacgact cggccttttc
     1861 catccctcaa tggaaaaaca actccgaatc gcgcaaaggt taagtaaccc gtgtcgccat
     1921 ttttgcaaac gatattgttc gccctgatga aacctacgct ttcaacttct ttgagatact
     1981 ccttcgtttt acgatgggtt atttgtcccg aactgaatcc tgcaccggtt acttcgcaag
     2041 aaagccctct ttctttcagc caagttctcg gctcttcgga ttttacgttc ctgattccat
     2101 tgtaaagcgt ttgataaatc ctttcgcaaa tttccgcgtg aagtttttgt ttttgcattt
     2161 ctatcatcat gattcctcct cctgatttct gcgttcttct tccgcgtgat tataatcgtc
     2221 aaccctttct attaaccgca tcatttccat gcgctgtttg ggttcataga ct
//
LOCUS       DAOMYG010000485         2270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_18274_length_2270_cov_7.144470, whole genome shotgun sequence.
ACCESSION   DAOMYG010000485 DAOMYG010000000
VERSION     DAOMYG010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18274_length_2270_cov_7.144470"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..940
                     /locus_tag="PK610_12840"
     CDS             245..940
                     /locus_tag="PK610_12840"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRJ36873.1"
                     /translation="MIRLLLLFSFLPVFAFQTTEFKLSNEEFLKKLQSLPDAQLVDVR
                     TQGEFRKNHLKGAMNLNVNGEEFQKQVSALDKTKPVLVYCWSGGRSSNAAEYMRQQGL
                     TVYEMKDGMMRWIAENKPYEEGTAPAAGMSMEEYNGYLKSKKLVLVDFNATWCAPCRK
                     MSPWLDELAKKYPNDLIVVKINTEENGTVVKTLNVAGVPYLMLYKNEKVIWTHSGLAE
                     KSVMEAEIEKALK"
     gene            complement(941..1330)
                     /locus_tag="PK610_12845"
     CDS             complement(941..1330)
                     /locus_tag="PK610_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36874.1"
                     /translation="MRSVVNFVVITLFFPIHVNAHQLSVNNESGEFKAGLEGMLGFSF
                     GKNFYSMNVGGPGLYFVINKNLKMGIGAFPSLYVLNGNLGARLGVSPRIDFKNLVLIT
                     PFFHRDRTDEWIWSVGFGYKFHRTPNK"
     gene            complement(1401..>2270)
                     /locus_tag="PK610_12850"
     CDS             complement(1401..>2270)
                     /locus_tag="PK610_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ36875.1"
                     /translation="DQQFDASASNQNFSGVLTIAYKASEKFNAYATYSTSYKPIGLNL
                     GGLPTQNGEVMKDLALVKPEFVSHAELGFKSAPTKKSFLNVTVHATEIKDYQTLVQTP
                     EIGVNRGYLSNAELVRVIGAELDGGIRIGSSFTLSGSFAYTDGKYIKFTNAPVPIEEV
                     GGTQSFKDISGSVLPGISKYAGSLNGEYSHKGKLLGRSGRFFLAADGFYRSSFSSSPS
                     ASAYINIDAYALVNSRIGFRANEGLTISLWSRNLLNNNYFEQLLVAAGSAGHYAAVLG
                     DPRTYGITIRQSF"
BASE COUNT          698 a          432 c          459 g          681 t
ORIGIN      
        1 gaaaatcata caggaattca acgactcatt gtcgattaaa agcttttata aatagtaaag
       61 gtgggcggaa gtcccccttt tttatttgta ttccgctttg tttcttttaa tacatacttg
      121 tgtaagagtt acgataaagg aataaaaaaa acagtgaaca cataaagaat taccaataat
      181 aagtgacgaa tatcaccatt tgtttgttcg atttgtgttc tatttgcata caaaactgtt
      241 gaccatgatt cgtttattat tgttgttttc ttttctgccg gtgtttgcat ttcagacaac
      301 tgaattcaag ctttccaatg aggagttttt gaaaaaactt caatcacttc cggatgcgca
      361 gttagtggat gtgcgtacgc aaggggaatt cagaaaaaac catttgaaag gtgcaatgaa
      421 cctcaatgta aatggagaag agtttcagaa acaagtcagt gctctcgaca aaaccaaacc
      481 ggtgctggtt tattgctggt cgggtggaag aagttccaat gctgcagaat acatgcgtca
      541 gcaaggttta actgtttacg aaatgaaaga cggaatgatg cggtggattg ctgaaaacaa
      601 accctacgaa gaaggtactg cgcctgctgc aggaatgagc atggaggaat acaacggata
      661 cctgaaatca aaaaaattgg tcctcgttga ttttaatgct acctggtgcg ctccttgccg
      721 taaaatgtcg ccatggctcg atgaattggc aaaaaaatat ccgaacgatt tgatcgttgt
      781 aaaaatcaac accgaagaaa acggaactgt tgtaaaaaca cttaatgttg ccggcgttcc
      841 atacctgatg ctgtacaaaa acgagaaagt catttggaca cactccggtc tcgccgaaaa
      901 aagcgtaatg gaggcggaga tcgagaaagc gttgaagtga ttacttatta ggtgttcggt
      961 gaaatttgta tccgaatcca accgaccaaa tccattcatc cgttcggtca cggtgaaaaa
     1021 agggagtaat cagcacgaga tttttaaaat cgatccgtgg tgaaacacct aatcgagcgc
     1081 ccaaatttcc attcaaaaca tatagtgaag gaaatgcacc tattcccatt tttaagtttt
     1141 tattgattac aaaatataaa cctggccccc ctacattcat cgaatagaag tttttgccga
     1201 aactaaaacc aagcattcct tcaaggcctg ccttaaattc tcccgattcg ttgttaaccg
     1261 atagttgatg tgcatttaca tgaataggaa agaatagtgt aattactaca aaatttacta
     1321 ctgatctcat tgtaagaaat tagtttgtga tagaataagt ctgaggtaaa tccaatgata
     1381 gatttacctc agactatgtt ttagaatgac tgtcgaattg tgatcccgta tgttcttgga
     1441 tcacccaata ctgccgcata atgccccgca cttcctgctg ctacaagtag ttgttcaaag
     1501 taattgttat taagcagatt tcgcgaccaa aggctaatcg ttaatccttc attggctctg
     1561 aatccaattc tggagttgac caatgcatac gcatcaatat taatgtaagc agatgcagaa
     1621 gggcttgatg aaaatgagga tctgtaaaat ccatctgctg ccaaaaagaa acggccgctt
     1681 cgtccaagta gttttccttt atggctgtat tctccattta atgatcctgc atattttgaa
     1741 attccgggta aaaccgatcc ggaaatatcc ttgaatgatt gcgttcctcc aacttcttcg
     1801 atgggtacag gtgcattggt gaatttgatg tattttccat ctgtataggc aaaagatccg
     1861 gatagggtaa agctgcttcc aattctaatt cccccatcca attcagcacc aattaccctt
     1921 acaagttcag cattggaaag ataccctctg tttacgccta tttcaggtgt ttgtaccagt
     1981 gtttggtaat ctttgatttc agttgcatga actgttacgt ttaaaaaact ttttttcgtt
     2041 ggagcagatt tgaatccaag ttctgcatgt gaaacaaatt caggttttac aagcgcaaga
     2101 tctttcatta cttctccatt ctgagtagga agtccaccca gattcaatcc tattggttta
     2161 tagctggtag aataggtggc ataagcattg aatttttctg aggctttgta ggcaattgtg
     2221 agcacacccg aaaaattctg atttgaagct gatgcgtcaa attgttggtc
//
LOCUS       DAOMYG010000486         2265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_18339_length_2265_cov_8.879638, whole genome shotgun sequence.
ACCESSION   DAOMYG010000486 DAOMYG010000000
VERSION     DAOMYG010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18339_length_2265_cov_8.879638"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="PK610_12855"
     CDS             complement(<1..1036)
                     /locus_tag="PK610_12855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRJ36876.1"
                     /translation="MFAIVDIETTGGSPQQSRITEIAVYLTDGVKQLDAYETLVNPEQ
                     PIPPFITKLTGISDEMVASAPRFQDVSSQLARITKDAVFVAHNVAFDYGMIRHEYSEL
                     GMDFHRSLLCTVKSSRRLLPGYPSYSLGNLCKEVGISIEQRHRASGDALATMHLFHML
                     FEGHRDDLLQLVEEGTLRFTGDPLHDSILRELPEQTGIYYLHDANNEVIHVGSGTNIR
                     RKVLHHLIKAYSRKSFELKNSIASVTFEVTGSDLLAQIVEMAEIKRIRPIYNRRAKPL
                     SYKKQFLNNPLHYIIDKGPDRYTRSVIRITDEQVGWGSLPAAQPIHSTEELDQFIRYA
                     GIGSEFRTLIE"
     gene            complement(1104..1481)
                     /locus_tag="PK610_12860"
     CDS             complement(1104..1481)
                     /locus_tag="PK610_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36877.1"
                     /translation="MDAKQHLYYGLGLIAYAVAGIDGKVQREERETIQRLLSEGIKKV
                     DVDYDVADIIFQIVEKEKADFETAYNWGIDAFKLGSHHLTPGMKWVFLDILQSVSDAF
                     PDGAMAEGELIARFARDLRAIGS"
     gene            1543..1836
                     /locus_tag="PK610_12865"
     CDS             1543..1836
                     /locus_tag="PK610_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRJ36878.1"
                     /translation="MFVRIVKLSFREDKIEDFERLFEKNKIAIRNQPGCKGLRLLHDK
                     TNPCIFFTYSDWEKEEDLENYRQSELFSQVWGTVKPWFTNKAEAWSVDVQHSL"
     gene            complement(1840..>2265)
                     /locus_tag="PK610_12870"
     CDS             complement(1840..>2265)
                     /locus_tag="PK610_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRJ36879.1"
                     /translation="LQPEEKGTIIQASEEYDHLIPSFIFKSNQVLISVLPRDFSFIVE
                     ENLREIFDVFASLGVRVHLMQNSALSFSVCIDHNEDKFPDLLKRLQENYKVKYNETVE
                     LITIRHYDQATIDMLTNAKEILVEQKSRHTARFIVRPKL"
BASE COUNT          676 a          484 c          469 g          636 t
ORIGIN      
        1 cttcaatgag ggttctgaat tcacttccta ttccggcgta acgaatgaac tgatccagtt
       61 cttcagttga atgtataggc tgcgctgccg gtaaactacc ccaacctact tgttcatccg
      121 taatgcgaat aacagatcgc gtataccgat caggcccttt atcaataata taatgaagcg
      181 ggttattaag aaactgcttc ttgtacgaga gaggtttggc ccggcgatta taaatagggc
      241 gtatccgctt tatttcggcc atttcaacaa tctgagccaa taaatcgctg cctgttactt
      301 cgaaggtaac actcgcaatg gagtttttca actcaaatga ttttcgcgaa taagctttga
      361 taagatgatg caaaactttt cggcgaatat tggttcctga accaacgtga atcacttcgt
      421 tattggcatc gtgtaaatag tagattcctg tttgctcggg taactcgcgg agaatagaat
      481 cgtgcaaggg atcacccgta aaacgcagtg tgccttcttc aaccaactgc aataaatcat
      541 cgcggtgccc ttcaaacaac atgtgaaaca aatgcatcgt tgctaatgca tctccgctgg
      601 cacggtgacg ttgttcaatg gaaatgccta cttctttaca taaattcccc agtgaatagg
      661 atggatatcc gggtaataaa cgtcggctcg attttacagt acataacagg ctacggtgaa
      721 aatccattcc caattcgctg tattcgtgac gaatcattcc ataatcgaat gcaacgttgt
      781 gagcaacaaa aacagcatct ttcgtaattc gtgcaagttg agaggataca tcctggaaac
      841 gtggagcaga tgctaccatc tcatccgata ttccggtgag cttggtaata aacggcggaa
      901 taggttgttc aggattaaca agagtttcgt aagcgtccaa ctgcttaacg ccatctgtta
      961 agtaaacagc aatttccgta atacgggatt gttgcggact accgccggta gtttcgatat
     1021 cgacaatagc gaacatgtgc taaagatagc aagaatagat tcgtgcctta caatgcaatt
     1081 cgcgggatgc tttcaaaaac cccttatgaa ccaattgcac gaaggtcacg tgcaaagcgt
     1141 gcaataagct cgccctcggc catcgcgccg tcgggaaatg catcggatac agattgtaaa
     1201 atatcaagga aaacccactt cataccggga gtaagatgat gagatccaag tttaaatgca
     1261 tcgattcccc aattgtatgc tgtttcgaaa tctgcctttt ccttttcgac aatctgaaaa
     1321 ataatatccg ctacatcata atcaacatcc acttttttga ttccctccga aagtaatcgc
     1381 tgaatggttt cccgctcctc gcgctgtact tttccgtcga tacctgcaac ggcataagca
     1441 attaaaccaa gtccgtagta gagatgctgt ttagcgtcca tatgcatgaa attttaggtt
     1501 aagatagcaa ttttcttatt ttgccacaaa atgacaccta tcatgtttgt gagaattgtg
     1561 aaattatcgt ttagagaaga caagattgaa gattttgaac gactctttga aaaaaataaa
     1621 attgccatcc gcaatcagcc cggatgtaaa ggattacgtt tgcttcacga taaaacgaat
     1681 ccatgcatat tcttcaccta cagcgattgg gaaaaggaag aagaccttga aaactaccgg
     1741 caatcagagt tgttttcgca ggtttgggga acggtaaagc cctggttcac caacaaggca
     1801 gaagcatgga gtgttgatgt gcaacattcc ttataacttt tacagtttgg gtcgcactat
     1861 aaagcgcgca gtgtgtctgc tcttttgctc aacgaggatt tcctttgcat tggtaagcat
     1921 gtcgatagtt gcctgatcat aatgacgaat ggtgatcaat tcaacggttt cgttgtattt
     1981 taccttatag ttttcctgta aacgtttaag cagatcaggg aatttatctt cattgtgatc
     2041 gatacaaact gaaaaactca aagctgagtt ctgcataaga tgcacacgta ctcccagcga
     2101 tgcaaacaca tcaaaaatct cacgtaaatt ttcctcaaca ataaaagaaa aatcgcgggg
     2161 caaaactgag atcagtacct gattcgattt gaaaataaaa gatggtatca gatgatcata
     2221 ctcttctgaa gcctgaatga tggttccctt ttcctcgggc tgcag
//
LOCUS       DAOMYG010000487         2265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_18341_length_2265_cov_8.432127, whole genome shotgun sequence.
ACCESSION   DAOMYG010000487 DAOMYG010000000
VERSION     DAOMYG010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18341_length_2265_cov_8.432127"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="PK610_12875"
     CDS             <1..1676
                     /locus_tag="PK610_12875"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ36880.1"
                     /translation="DLATAAQPLAVVRQFGGNCSGGNGELDIYKSYFRDLEFTPAENL
                     GETINSSADDFGITIAKNGKTGFISSNREGGKGSDDIYFIQITETAVVESKDLQGRFS
                     YRNLDNGIKDLDVLLIDDSGRVVLRVRTNENGEFAFRNLPVSDNYSIKLVEPSADLKL
                     EIFDKNGEAIAVLRSDQNGKFLYKKLDAIQGGMLTMMEEEEPQFSTKGRLNGQFIFEN
                     DPNKYPDGLNVYLMDNNGNVKGTTKTDNRGNFSFRQLDLGDNYIIRTDSLGDDAIMLI
                     YNKHENVIAEYRRDKNGNYVFRKLGDRSSDLSLMDIESDSATFAQLSVSLNGQFRFRS
                     LDGTPSDMSFFILDDDGNLIYKGKTDENGHFRARNLAVADQYIFQIDPNDPNYDKKLI
                     VELFARNGKSLAVLENDKSGKFVYKRLKQEFSYIKEIKTEEVQLELPDKIPYIYFEKN
                     SSYLDEASKKQLDKVVQIMRKVPHLKIEISAHADSRADDEFNDWLTEKRMNRVKDYLI
                     DKGIPASRITGAYYGKRKPVNSCSSPTDCDEDQHRQNRRCELRLIPAKA"
     gene            1836..>2265
                     /locus_tag="PK610_12880"
     CDS             1836..>2265
                     /locus_tag="PK610_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36881.1"
                     /translation="MTLFVPEGGFPIGNEIVIKYPTFDKFIHSRKKERVKIDDLLAGM
                     DTTMKEEIPADTFIRHNNKIGTELGAPSDTSTGTVDASRLQRIEFGPQGPTVLHSIFQ
                     KLGEASAAKKNVRILHYGDSQIEGDRITGYFRQRLQAKFGG"
BASE COUNT          725 a          460 c          482 g          598 t
ORIGIN      
        1 acgacctcgc cacggcagcg cagccgctcg ccgtcgtacg acagttcggc ggcaactgca
       61 gcggaggaaa tggcgaactc gatatttata aatcctattt cagggacttg gaatttactc
      121 ctgcagaaaa cctcggtgaa accatcaact caagtgccga cgattttgga attactatag
      181 ctaaaaacgg gaaaaccggt tttatttcct ccaacagaga aggcggaaag ggcagcgatg
      241 atatttattt tattcagata acagaaacag ccgttgtaga atcaaaagat cttcaaggtc
      301 gtttctccta ccgtaatctc gataacggga taaaagatct ggatgtacta cttattgacg
      361 acagtggaag agtggtcttg cgtgtaagaa ccaacgaaaa tggtgagttt gcattcagaa
      421 acctgcctgt atccgataat tactccatta aactggtaga accttcggcc gatttaaaac
      481 tggaaatttt tgacaaaaac ggtgaggcga ttgccgtgtt gcgatcggat caaaatggta
      541 aatttctcta taaaaaactc gatgccatac agggaggaat gcttactatg atggaagagg
      601 aagaacctca gtttagtaca aaaggaagat taaacggcca attcattttt gaaaatgacc
      661 ctaacaaata tcctgacggc ttgaatgttt acctgatgga taacaatggg aatgtaaaag
      721 gaacgacgaa aacagataac agaggtaatt tcagttttcg tcaacttgat ttaggcgata
      781 attacatcat cagaacagat agcttgggtg atgacgccat catgcttatt tacaataaac
      841 atgaaaatgt aattgctgaa taccgtcgcg acaaaaacgg taattatgtt ttccggaaac
      901 ttggagatcg ttcatccgat ctttcattga tggacatcga atcggattct gcaacatttg
      961 cgcaactttc ggtatcgctg aatggccaat tccgttttcg ttcgctggat ggcacaccaa
     1021 gtgacatgag ttttttcatt ttggatgatg atggtaattt aatttacaaa ggcaagaccg
     1081 atgaaaacgg acatttcaga gcgagaaatc ttgctgttgc tgatcaatac atctttcaaa
     1141 ttgatcccaa cgaccctaac tacgataaaa aactgattgt tgaattgttt gcacgtaatg
     1201 gaaaatctct tgctgtactt gaaaatgaca aatcaggaaa attcgtttac aaacgcttga
     1261 agcaggaatt cagttacatc aaagaaataa aaacagaaga agttcaactg gaactcccag
     1321 ataaaattcc ttacatctat tttgaaaaaa attcaagtta tcttgatgag gcttccaaaa
     1381 aacaacttga taaggttgta caaatcatgc gtaaagtacc acatctgaaa attgaaatca
     1441 gcgcccatgc cgactcaaga gcagatgatg aatttaatga ttggttaacc gagaagcgaa
     1501 tgaaccgtgt gaaggattat ttaattgata aaggaattcc tgcttctcga atcacaggag
     1561 cttattacgg aaagcgaaag ccggtaaaca gttgcagctc acccacagac tgtgacgagg
     1621 atcagcaccg gcaaaacaga cgctgtgagc tacgcctaat cccggccaag gcttgagtgt
     1681 tagagtcctt gtcaaaaact tttccatctc tcccccatct cttgcctgct tctttctact
     1741 tttgatcaac aggccagaat tctaactcat tggaagcgta tagtcccctt cgggttttcc
     1801 ttttcatttt tgcggttctc agcatattgc tgagcatgac cctgtttgtc ccggaaggcg
     1861 gctttcccat tggaaatgaa attgttataa agtaccccac gtttgacaag ttcatacatt
     1921 cccgaaaaaa agaaagggta aaaattgatg atctcctggc cgggatggat actacgatga
     1981 aggaagaaat tccggctgac acgtttattc gtcacaacaa caaaataggc actgagcttg
     2041 gcgcacccag tgatacctct accggaacag ttgatgcatc gcgcttacaa cgtattgaat
     2101 tcggacctca ggggcctaca gttttacatt cgatttttca aaaactagga gaggcatcgg
     2161 cagcgaagaa aaatgtgcgc attcttcatt acggcgattc gcaaatagaa ggagaccgaa
     2221 tcacaggata tttcagacag cgtttacaag ccaaattcgg aggta
//
LOCUS       DAOMYG010000488         2247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_18557_length_2247_cov_8.918796, whole genome shotgun sequence.
ACCESSION   DAOMYG010000488 DAOMYG010000000
VERSION     DAOMYG010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18557_length_2247_cov_8.918796"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..907)
                     /locus_tag="PK610_12885"
     CDS             complement(62..907)
                     /locus_tag="PK610_12885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HRJ36882.1"
                     /translation="MNTTSKTPITSNYIELLKKVLIDHRNIGEFEYYPLPIENANWKT
                     AFLYPVDKLLRKRNFIIAKRKPVIHEERMNGYDWPAQALTMIGYNRLTNIEQCIHAII
                     QDKVEGDLIEAGVWRGGAALFMRAMLKELSVTNRKVWLADSFRGLPKPDAVNYKADKW
                     NRLNRLKILNVGMDEVKHNFEKYDLLDDQVKFLPGWFKDTLPEAPIEKLSLIRLDADM
                     YESTWQALENLYPKLYPGGFVIVDDYYAFKNCKQAVDEFREKAGITEPTIDIDLEAIY
                     WRKAK"
     gene            complement(919..1398)
                     /locus_tag="PK610_12890"
     CDS             complement(919..1398)
                     /locus_tag="PK610_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HRJ36883.1"
                     /translation="MSRSVYLANRIREVYLNGKWIANTNYKEQLSGLNADQAAHIAGD
                     LNSIAVLTYHINYYLVGILNAMESGILDIRDQYSFDGPEVTTDVDWNKLLTEFLANAE
                     CFADKIERLKEEELDKDFFAEKYGTWLRNIDGLIEHSYYHLGQIVLIRKLIAMPTAH"
     gene            complement(1395..1568)
                     /locus_tag="PK610_12895"
     CDS             complement(1395..1568)
                     /locus_tag="PK610_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36884.1"
                     /translation="MKKAILIVMLVLNAIVLLGQLWPEGAPPFAGIVNLITLGLNLVM
                     FLILFRKENLFTR"
     gene            complement(1592..1939)
                     /locus_tag="PK610_12900"
     CDS             complement(1592..1939)
                     /locus_tag="PK610_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HRJ36885.1"
                     /translation="MINIPFQTINWSAIQKEEYPGETGTAIWQTLQLGGLRIRIVEYS
                     EGYLADHWCEKGHIVHCLEGEFVSERLSGEKDVLKAGDSFVVSDNLSSHRSQSINGVK
                     LLIIDGDFLKHFE"
BASE COUNT          660 a          523 c          367 g          697 t
ORIGIN      
        1 ctgtatctca cacttggaga atcgagattg taataggcaa accaataaat aaactgcgtt
       61 ttcatttagc ttttctccag taaattgcct cgagatcaat atcaatagtc ggctcagtga
      121 ttcctgcctt ctcccgaaac tcatcgactg cctgcttaca gtttttgaaa gcatagtaat
      181 catcaacaat gacaaaccct ccgggataaa gtttgggata caaattttcc agcgcctgcc
      241 aggtggattc gtacatatcc gcatccaaac gaattaagga aagtttttca ataggagctt
      301 caggcaaggt gtctttaaac catccgggca aaaactttac ctgatcatct aacagatcat
      361 atttctcaaa attatgtttc acttcatcca tcccaacatt gagaattttt aatcgattga
      421 gacgattcca tttatctgct ttataattta ctgcatcagg tttaggtaat ccccggaaag
      481 aatccgctaa ccataccttt cgatttgtaa cggaaagctc tttcagcatt gcacgcataa
      541 acaaggccgc gccccctctc caaacaccgg cttcgatcag atcgccttct actttatcct
      601 gaataatggc atgaatacat tgttcaatat ttgtcagcct gttataacca atcatagtaa
      661 gcgcctgagc cggccagtcg taaccgttca ttcgttcttc atgaataact ggtttccgct
      721 ttgcaataat gaaatttcgt tttcgcagta atttatcgac aggatacaaa aaggctgttt
      781 tccagtttgc gttctcgata ggtaacgggt aatattcgaa ttcgccaata ttccggtgat
      841 cgatcagcac tttttttaaa agttcaatgt agttggatgt tattggtgtt tttgatgtcg
      901 tattcattgc atcaaaaatt agtgtgctgt tggcattgca atcaatttcc ggatcaaaac
      961 aatctgacca agatgataat aactgtgttc aatgagtccg tctatatttc gcaaccatgt
     1021 cccgtatttt tctgcaaaga agtccttgtc caactcctcc tctttaaggc gttcaatttt
     1081 atctgcaaaa cattctgcat tagcaagaaa ttcagtaagc agtttattcc agtccacatc
     1141 tgttgttact tcaggaccat cgaaactgta ttgatcacgg atatccagaa ttccactttc
     1201 catggcattc agtataccta ccagataata attgatgtga tacgtaagga cagcaattga
     1261 attcaaatct cctgctatat gggcggcctg atcagcattt aatccggata attgctcctt
     1321 gtaattggta tttgcaatcc atttaccatt aagataaact tcccggatcc gattggccag
     1381 ataaacgctt cgactcatcg tgtaaacagg ttctccttgc ggaacaaaat caaaaacatc
     1441 accaggttta atccaagtgt aatcagattt actatcccgg caaatggagg agctccttcg
     1501 ggccaaagtt gacctagtaa tacaatagca tttaaaacta gcataacaat caaaatagcc
     1561 tttttcatat tcattctatt taataataaa cctactcaaa atgctttaga aaatccccat
     1621 caatgattaa aagttttact ccgttaattg attgagaacg atgtgagctc aggttatccg
     1681 aaactacaaa tgaatccccc gcctttaata cgtccttctc acccgacaaa cgctcggaaa
     1741 caaattcgcc ttccaaacaa tgaacaatat gacccttctc acaccaatgg tccgccagat
     1801 acccttccga atattccaca atccttattc gaagcccccc taattgtagg gtttgccaga
     1861 ttgcagttcc ggtttcaccg ggatattctt ctttttgaat tgcgctccag ttgattgttt
     1921 gaaaggggat gttgatcatt taaaagtttt tttgcgttct gtttaaaagc agcgttacaa
     1981 atttatctaa gggaactgca ttaatgtaat ctacaaaata gttggcaatc cctttccccc
     2041 aaggcaccta atttccttca acccgacata tctattgaga cattgttcat gcattagctt
     2101 attgattcta atcaggctat tgcagaaaca ccttccacat ctcacatacc tatctttact
     2161 ttaccatctg ccctacccaa taaaccggta aagcagcaga attctactct cattcccacc
     2221 tgtccttacc aagacatcag ggttctc
//
LOCUS       DAOMYG010000489         2245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_18573_length_2245_cov_7.105936, whole genome shotgun sequence.
ACCESSION   DAOMYG010000489 DAOMYG010000000
VERSION     DAOMYG010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18573_length_2245_cov_7.105936"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2089)
                     /gene="recG"
                     /locus_tag="PK610_12905"
     CDS             complement(<1..2089)
                     /gene="recG"
                     /locus_tag="PK610_12905"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRJ36886.1"
                     /translation="MALLPDTAIEYMKGVGPQRAESLKKELGIHTCWGLLNFFPYRYI
                     DRSKFYTVSELHPDLPYVQLRGRITNIESIKGKGSRLKAIFRDETGSVELVWFQGAKS
                     VESALKPGEDYVLFGKPNFFRDRYSIPHPELELLSHFNAGIKGNLQGLYSSTEKATQK
                     GLNSRGIEKLMRVLVPEISGSIHEIFPASILSRYALMDRRTAYEQIHFPASVEQMVRA
                     QFRMKFEELFFLQMQLVRLKLTQTKKAKGFVFGEVGEVFNQFYKEFMPFELTNAQKRV
                     VKEIRKDMGTGAHMNRLVQGDVGSGKTVVALLSMLIAIDNGFQACLMAPTEILAIQHY
                     QTLTEMLGLMPVNVRLLTGSSKTSERNEILKGIAAGTIHILIGTHALIEPVVEFKNLG
                     LAIIDEQHRFGVEQRFKLWKKNTTPPHVLIMTATPIPRTLAMTLYGDLDVSVIDELPP
                     GRKPVKTSHHFDSARIKVFGFMEEQIKLGRQIYVVYPLIEESEKLDFKDLMDGYESLS
                     RRFPLPDYQISVVHGKMNPAAKEIEMQRFADGKTHIMVATTVIEVGVNVPNASVMVIE
                     SAERFGLSQLHQLRGRVGRGADQSFCMLMTGNKLSNDARLRMETMCRTNDGFEIAEVD
                     LKLRGPGDLMGTQQSGALDLRLADLAKDGDIVAASREAAQLLLEKDPDLTLPENQLVR
                     QRLAQMVKDRPNWS"
BASE COUNT          621 a          533 c          399 g          692 t
ORIGIN      
        1 tgctccaatt cggacgatcc tttaccattt gcgccaatcg ttgtcgcacc aattgatttt
       61 caggaagcgt caaatccgga tccttttcca ataaaagttg tgcagcttct ctcgatgcag
      121 caacaatatc tccatctttt gcaaggtctg ccaaccgtaa atccaatgca ccactttgtt
      181 gagttcccat taaatcgccc ggaccacgaa gtttcagatc cacttctgca atttcaaatc
      241 cgtcgttggt tctgcacatg gtttccattc gcaatcgcgc atcattactg agtttgttcc
      301 ctgtcattaa catacagaat gactgatccg ctcctcttcc taccctacct cgcaactgat
      361 gcaattgtga caaaccaaat cgttctgcgc tttcgatcac catcacactt gcattgggaa
      421 cattaactcc cacttcaatt acggtagttg caaccatgat gtgtgttttt ccatctgcaa
      481 atcgttgcat ttctatttct ttcgctgccg gattcatttt accgtgtacc acactaatct
      541 ggtaatcggg taacggaaag cgacgcgata aactttcgta tccatccatt aaatctttga
      601 aatcaagttt ttcggattct tcaattaatg gatacaccac ataaatttgt cgtcccagtt
      661 ttatttgttc ctccatgaat ccgaaaactt ttattcgtgc agaatcaaaa tggtgagatg
      721 tttttacagg ttttcttccg ggtggcaatt catcaatgac tgaaacatca agatctccgt
      781 aaagtgtcat agccagtgtt cgcggaattg gagtagctgt catgatgagt acatgcggcg
      841 gtgttgtatt ctttttccag agtttaaacc gttgttccac accaaaacga tgttgctcat
      901 caataatggc aagaccaaga tttttgaatt caacaaccgg ttcaatgagt gcatgcgtcc
      961 caatcaaaat atgaatcgtt cctgcagcta ttccttttaa aatttcattg cgttccgatg
     1021 tttttgatga cccggtgagc agtcgaacat tcacaggcat cagtccgagc atttcggtta
     1081 acgtttgata atgctgtatg gcaagaattt cagttggggc cattaaacaa gcctgaaatc
     1141 cattatcaat ggcgataagc atacttaata atgcgacaac agtttttcct gaaccaacat
     1201 ctccctgaac caatctgttc atgtgtgcgc cggtacccat atccttgcga atttctttca
     1261 ccacccgttt ttgcgcattg gtaagttcaa acggcatgaa ctccttgtag aattgattga
     1321 acacttcacc cacttcacca aatacaaatc cttttgcttt ttttgtttgt gtaagtttaa
     1381 gccgcacaag ctgcatttgc agaaaaaaca attcttcaaa tttcatccgg aactgggctc
     1441 tcaccatttg ctctactgaa gcaggaaaat gaatttgttc ataggctgtt ctacgatcca
     1501 tcaatgcata acgggaaaga atagaagccg gaaaaatctc atgaatactg ccggaaattt
     1561 caggaacaag aacgcgcatc agtttttcaa ttcccctgct gttcaatcct ttttgtgttg
     1621 ccttttctgt tgatgaatat agtccctgta gatttccctt aataccggca ttaaaatgcg
     1681 ataataactc caattcagga tgcggtatcg aatagcggtc acgaaaaaaa ttcggtttcc
     1741 caaacaacac ataatcctcc ccgggcttta gggctgattc cacagacttt gcgccctgaa
     1801 accaaaccaa ttccactgat cctgtttcat ccctgaaaat tgctttaaga cgcgaaccct
     1861 ttccctttat cgattcgata ttggttattc gtccgcgcaa ctgtacatag ggcagatcgg
     1921 gatgcaattc tgaaaccgta taaaacttgc ttcgatcaat gtacctgtag gggaaaaaat
     1981 ttagcaagcc ccaacacgtg tgaataccca attctttctt gagggattcc gctctctgcg
     2041 gaccaacccc cttcatgtat tcaattgcag tatcgggcaa tagcgccata gtccaaaaat
     2101 acgtttcaat ttcttgtatt taaaggaatt cttatcagat tgtcaacagt ttagcattga
     2161 aaatacctgc tattcaatga agaacacaat tgtaaatcct ttcacaattg cataagtaat
     2221 cgtgaatttc cctcatttgg aagca
//
LOCUS       DAOMYG010000490         2243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_18605_length_2243_cov_7.908135, whole genome shotgun sequence.
ACCESSION   DAOMYG010000490 DAOMYG010000000
VERSION     DAOMYG010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18605_length_2243_cov_7.908135"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="PK610_12910"
     CDS             <1..1056
                     /locus_tag="PK610_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HRJ36887.1"
                     /translation="GDRITGYFRQRLQAKFGGNGPGMIAAFNQYHTMSFVQTVSPNFK
                     RHTVFGEVSKEITHKKYGSMGTFARFTKYVPDSLLSTLESTEGWIEIGMHKQAYGNSK
                     KFNRVRLFYGNCRSRVEMIVSNNGQVIHEDTLIPDGNYHVVELLFENTPEQLRYTFKG
                     KDSPDFYGFSLDGNYGVTIDNVAMRGGSGTFLGKCDGNLMSRMYSDLGVEMFIMQFGG
                     NTMPYLKDAAASKQYANYFQSNLNTLKRLRPGASIIVIGPSDMSTKVDGEYMSYPLLG
                     TLIADMKQAAFNAGAGYWDIYSAMGGENSMLTWVEKGLAGEDYTHFSPSGTRIIAEKF
                     WEAFLYEYQQYQKGVQP"
     gene            1053..>2243
                     /locus_tag="PK610_12915"
     CDS             1053..>2243
                     /locus_tag="PK610_12915"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRJ36888.1"
                     /translation="MRNGWIIVLLLLSCFSGAVKAQWDDDLLNDSLMGIIDTAKIRQY
                     PFIHYGKNRYDFPSGNSYAFERFYSKFDSVINRRKGQVVIYHIGGSHIQADIYSNKMR
                     TYLNSYWPGLLGARGLVFPFSLAETNNPYNYKAEGTGNWTAVRNVEKKDTSRLGLLGI
                     SIRTKDSISSIKIYYREKESMRYLHDQFRVYHNSNPAYKVHFAYPGLVKSTEVDSIAG
                     YTQFNLYQEVDTAWFVIQRLHSDTGAFVCYGVEMMNEQPGIVYNSIGINGASFNSYKR
                     CQDWEKQLSARKPDMFIISIGTNDANVLYNDFDSVGFYQRYEEMILKILHVNPDAAIL
                     LTVPNDAYYYRKYANKNVARMEQIIFALAEKYNMGVWDFYGIMGGYNSSHAWYKEGLM
                     HKDRV"
BASE COUNT          716 a          442 c          491 g          594 t
ORIGIN      
        1 ggagaccgaa tcacaggata tttcagacag cgtttacaag ccaaattcgg aggtaacggc
       61 cccggcatga tcgctgcatt caatcaatat catacgatgt cattcgttca aactgtatct
      121 cctaatttta aacgacacac cgtatttggt gaagtaagca aggagataac ccataaaaaa
      181 tacggcagca tgggcacctt tgcccgattt acaaaatatg tacccgattc attgctgtcc
      241 acacttgaaa gcacggaagg atggattgaa atcggcatgc acaaacaagc ctatggcaat
      301 tccaaaaaat tcaatcgcgt tcggctattc tatggtaatt gcagatcacg agttgaaatg
      361 attgtaagca ataacggtca ggtcatccat gaagacacac tcataccgga tgggaattat
      421 catgtagttg aattgctttt tgaaaacaca ccggaacaat tgcgctacac attcaaagga
      481 aaagacagtc cggacttcta cggtttttcc cttgatggaa attacggagt aaccattgac
      541 aatgtggcta tgagaggagg atcaggaaca tttttaggaa aatgcgacgg aaatctgatg
      601 agcagaatgt acagtgattt aggcgttgaa atgttcatta tgcaatttgg cggaaacaca
      661 atgccctacc tgaaagacgc agctgcatca aaacaatacg ctaattattt ccagagcaac
      721 ctcaacacat taaaacgttt gcgaccggga gcatccatta ttgttatcgg cccatcggat
      781 atgtctacaa aagtcgacgg cgaatacatg agctatccct tgcttggcac tttaattgct
      841 gatatgaaac aggccgcctt taatgcaggc gcaggatatt gggatattta ttcagctatg
      901 ggaggagaaa attcaatgtt gacatgggta gaaaaaggac ttgcagggga agattatact
      961 cacttctctc cttcaggtac gcgaatcatc gctgagaaat tttgggaagc ttttttatac
     1021 gaataccaac aataccagaa aggagttcag ccatgagaaa cgggtggata atcgttttgt
     1081 tgttgttgag ttgtttttcc ggtgcggtga aggcacaatg ggacgatgat ttactgaacg
     1141 atagtttgat gggaataatt gataccgcga agatcaggca gtatccattc attcattacg
     1201 gaaaaaaccg ttacgatttt ccgtcaggca attcctatgc tttcgaacga ttttattcca
     1261 agttcgacag cgtgataaat aggagaaaag gtcaggtggt tatttatcac attggtggtt
     1321 cacacattca ggccgacatc tactcgaaca agatgcgaac ctatctcaac tcctattggc
     1381 ccggattatt aggcgcaagg ggattagtgt ttcctttcag cctggcagag acaaacaatc
     1441 cttacaacta caaagcagaa ggcaccggta attggacagc agtaagaaat gtagaaaaaa
     1501 aagacaccag tcgattagga ttacttggga tatcaattcg caccaaagat tcgatttctt
     1561 ctataaaaat ttattaccgc gaaaaagaaa gtatgcgtta tctgcacgat cagtttcgcg
     1621 tttatcacaa ctcaaatcct gcgtacaaag tccactttgc ttaccccggc ctggttaaaa
     1681 gcaccgaagt agacagtatt gcaggataca cgcaattcaa cctttaccag gaagtagata
     1741 cagcgtggtt tgttattcaa cgcttacact ctgataccgg tgcgtttgtt tgttatgggg
     1801 tagaaatgat gaatgaacaa ccgggaatcg tttacaactc cattggaata aacggagcat
     1861 cgtttaacag ctacaaaaga tgtcaggatt gggaaaaaca actttcggcg cgtaaacccg
     1921 acatgttcat catttctatt ggaacgaatg acgcaaatgt tttgtacaat gattttgatt
     1981 cggtcggatt ttatcagcga tatgaagaaa tgatccttaa aatattacac gttaatccgg
     2041 atgcagcaat attactaacg gtacccaacg atgcgtatta ctatagaaaa tatgccaaca
     2101 agaacgttgc ccgcatggag cagatcattt ttgcattagc agaaaaatac aacatgggcg
     2161 tttgggattt ttacggaatt atgggaggat ataattcttc ccacgcctgg tacaaagaag
     2221 gtttaatgca caaagacagg gtc
//
LOCUS       DAOMYG010000491         2234 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_18704_length_2234_cov_9.419917, whole genome shotgun sequence.
ACCESSION   DAOMYG010000491 DAOMYG010000000
VERSION     DAOMYG010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18704_length_2234_cov_9.419917"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PK610_12920"
     CDS             complement(<1..231)
                     /locus_tag="PK610_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36889.1"
                     /translation="MPKTILFLLFVTLFRFSGAQQTLPYTFHHINPNAFGLKDVFGQN
                     FDSKGRMWMVCSEGLAVYNGYSASVYRSDSADA"
     gene            complement(344..1261)
                     /locus_tag="PK610_12925"
     CDS             complement(344..1261)
                     /locus_tag="PK610_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36890.1"
                     /translation="MKRRLVKGLLGVALVLIALELVARFYFGLGRLPVYVEHERYEYM
                     FAPNQDVWRFGNHVLTNEFGMRSASPLASDSIRILKFGDSVINGGAHIDQDSLSSTLL
                     EKQLSDDFGKPVRVLNISAQSWGPDNAFAFLQEHGDFNTDLIILVFSSHDLHDNMHHQ
                     KVVGEKRAWPSKQPLCALTDAWSRYAWPWMQRKMGMETDEYDYFLGFDDSKVNPGWEQ
                     FIRYSKEKNIPLIVYLHPEQSELKSKTYNKYGIEILNLLQKDTVSVIKGIETIRDRSC
                     YRDNVHLNEKGHRKLAEALLPGVRSYFNH"
     gene            complement(1258..>2234)
                     /locus_tag="PK610_12930"
     CDS             complement(1258..>2234)
                     /locus_tag="PK610_12930"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRJ36891.1"
                     /translation="EAFLKDMFYFLLSIVVIGILIRFYNAEIAFSHGGRMRGIFGNPN
                     GLGIFLILTFLFFSVTNHLHPEMYSKNEKLFIYAILFYSVYKTGSRSALLAIVFYLAF
                     IRVYKVSPFLGFIAFLSSILGYELFLQNYATLIRALGLETVFRLDTLEEGSGRLIAWT
                     FAWENIQQSMFLGKGISYDEYLMRSNFDYLSKQGHEGGVHNTYLIMWLNTGLIGLLGF
                     LRGLLLTFIRAGKNTPIAFPVMFAVMFSINFEPWLAASLNPFTILFLSIATVLTGDAF
                     EEQWNRVKSTSTPLSGHVENENGGENGTSLPGLDVHGDKPGNLKDSWV"
BASE COUNT          721 a          531 c          416 g          566 t
ORIGIN      
        1 cgcatcggca gaatcggagc ggtaaacaga agcagagtat ccgttataaa cagcaagtcc
       61 ttcggaacat accatccaca ttcttccctt actgtcgaaa ttctgaccaa aaacatcttt
      121 caggccaaag gcattgggat tgatgtgatg aaaagtataa ggcaatgtct gctgcgcccc
      181 ggaaaaccgg aatagcgtta caaatagcag aaacagtatc gtttttggca caagcgaata
      241 taggggaaaa ttgattttat gccaactatt ttgcttcacc actaagacac ttagttcact
      301 aagaaacact aagtacttag tgcaccttgg tggccttagt gccttagtgg ttaaaatacg
      361 atctaacccc aggcaacaaa gcctccgcca acttacggtg ccctttttca ttgaggtgaa
      421 cattatccct gtaacaggag cgatcccgaa tcgtttcgat gccttttatt acgcttacag
      481 tatccttttg tagcaaattg agtatttcta taccgtattt gttatacgtt ttagatttta
      541 gttcggattg ttcaggatgc aggtaaacga ttaagggaat gtttttctct ttggaatacc
      601 taatgaattg ttcccatccc ggattcactt tagaatcgtc gaagcccagg aaataatcgt
      661 attcatcagt ttccattccc atttttcgtt gcatccaggg ccaagcgtaa cgtgaccatg
      721 catcggtcaa tgcgcataag ggttgctttg atggccaagc tcttttttca ccaaccactt
      781 tttggtgatg catattatca tgaagatcgt gagaagaaaa aacgagaatg atcagatcgg
      841 tattaaaatc accatgctcc tgcaaaaagg caaaggcatt atcgggaccc caactttgcg
      901 cggaaatatt gagcacgcga acaggttttc cgaaatcatc cgacaattgt ttttccaata
      961 atgtcgatga caagctatcc tgatcaatat gtgctccccc attgattacc gaatcaccga
     1021 atttcaaaat gcgaatgcta tccgaagcaa gaggcgaagc ggaacgcata ccaaattcat
     1081 tagtcaacac atgatttcca aagcgccaaa catcctgatt gggagcgaac atgtattcgt
     1141 agcgttcatg ctccacatac accggtaaac gtcccaaccc aaaataaaac cgcgccacca
     1201 gttccaaggc aatcaacacc aatgcaacac caagcaatcc tttcactaat ctgcgcttca
     1261 tacccaacta tcctttaaat tcccgggttt atccccatga acatccaatc ccggcaaaga
     1321 agtaccgttc tcgcccccat tctcgttctc cacatggcca ctgagcggag tcgaagtgga
     1381 ctttacccta ttccactgct cctcaaatgc atctcccgtt agtaccgttg caatcgataa
     1441 aaacaaaatt gtaaacggat tcaaagaagc cgccagccag ggttcgaagt tgatggaaaa
     1501 catcaccgca aacattaccg gaaatgcaat gggtgtattc ttacccgccc gaataaaagt
     1561 cagtaacaag ccacgtaaaa aaccgagcaa tccgatcaaa cctgtattca gccacatgat
     1621 gagatacgta ttgtgaacac cgccttcgtg accttgttta gacaaataat cgaaattgga
     1681 tcgcattagg tattcatcat aggaaattcc tttacccaaa aacatgcttt gctgaatgtt
     1741 ctcccatgca aaggtccagg cgatcagtcg accggatccc tcctccaagg tgtccagacg
     1801 aaaaactgtt tccaatccca gcgcacgaat taaggtggca taattctgca aaaagagttc
     1861 gtaacctaaa atggatgaaa gaaaagcaat gaaacctaaa aaaggagaaa ctttatatac
     1921 acgaataaat gccaggtaaa acacaatagc aagcaatgca gaacgcgatc ctgttttata
     1981 caccgaataa aaaagaatcg cgtaaataaa cagtttttcg tttttcgaat acatctccgg
     2041 atgaaggtgg tttgtaactg aaaaaaacaa aaatgtaagt atcagaaaaa ttcccaggcc
     2101 gttgggattt ccgaaaatac cacgcattct ccccccgtgc gaaaaggcaa tttctgcatt
     2161 gtaaaaacga atcagaattc caatcacaac tatactcagc aaaaaataaa acatatcctt
     2221 cagaaatgct tccc
//
LOCUS       DAOMYG010000492         2213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_18975_length_2213_cov_7.529657, whole genome shotgun sequence.
ACCESSION   DAOMYG010000492 DAOMYG010000000
VERSION     DAOMYG010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18975_length_2213_cov_7.529657"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..1034
                     /locus_tag="PK610_12935"
     CDS             207..1034
                     /locus_tag="PK610_12935"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HRJ36892.1"
                     /translation="MRHRIFLTLFLLAVVQLPVQSQIISLLNENFNSGFPAGWINSDE
                     DACTPAPAVNLITGDAFKTHIDYDTLTGGDSILVATSWFQPACQASNYLVLPALTLQA
                     NGNLFQFQYKSKDPSYPEAFEVLLSTTGTAWVDFSDTLFSTNLANPNWVNASIDLDAF
                     AGQTVYLAIHHSSNDKFILCLDNFKVFADLQLSVEEITDPQVKLFPNPVSDLLSIQST
                     AGIESVFIYDAAGKLLMSTESNSSGFVSLTVNQLPAGFYFARVNTSYGSITRQFIRH"
     gene            1049..1690
                     /locus_tag="PK610_12940"
     CDS             1049..1690
                     /locus_tag="PK610_12940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ36893.1"
                     /translation="MHIELNQALPLPMRDESFSPESLWKRSAILESGNDHLILAPSGT
                     GKTSLLSFLFGMRNDYQGSILMDNKEVKTISIAEWALIRRERISIVMQDMRLIPTLTV
                     EDNLNLKNNLKKMYRENDIRDMLSRLGLQEKWKQKAGTLSIGQQQRVSIVRSLLQPYE
                     LLMLDEPFSHIDEENIRLASELIVEQTRKKGANILMVSLGLDYNMKFNQQHRL"
     gene            1694..>2213
                     /locus_tag="PK610_12945"
     CDS             1694..>2213
                     /locus_tag="PK610_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36894.1"
                     /translation="MMRKVLLKSQRPLQLIVAMLGALAGVFMLLFSIQVYLDLSAALN
                     AREDLLGSDYLVIQKQVTTLDSFNPESLGFGTNEIDDIRHMNFVEEVGVFESSRFQVF
                     AAITGLPGSREIYTEAYFEAVPDRFLDVVTGDWQWKEGDERVPVILPAAYLDAFNFGF
                     GPSQGLPPLTEKA"
BASE COUNT          589 a          465 c          466 g          693 t
ORIGIN      
        1 ataggataat cagccttttc cgcttgcgaa tcatccattt ttgaagtttt acgagctcaa
       61 tctgcaattt catgaactct tcttcgaatt ccttatcggt ttctaaatgc tctctcatta
      121 ggataaaggt aaatatttac cccattcgtg tcgcattctg caatatttca agggcttttg
      181 cgttgatttt cttacttttg ttacccatga gacaccgcat ttttctgacc ctttttttac
      241 tggctgttgt tcagctgcct gtccaatcgc aaatcatcag cttgctgaac gaaaatttta
      301 attccggatt tccagccggg tggatcaaca gcgatgagga tgcctgtact ccggcacctg
      361 ctgtgaatct tattacagga gatgcattca aaactcatat cgactacgat acccttaccg
      421 gtggggatag tattctggtt gcaacttcct ggtttcagcc ggcctgtcag gcaagcaatt
      481 atttggtttt gccggcgctt accttgcagg caaatggaaa tctctttcag tttcaataca
      541 aaagcaaaga cccttcctac ccggaagcat ttgaagtgct tctttccaca acaggtacgg
      601 catgggtaga tttttctgat acgctctttt caacgaatct cgctaaccca aattgggtga
      661 atgccagcat cgatctggat gcctttgcag ggcaaaccgt ttaccttgct attcaccatt
      721 cttcgaatga taaattcatt ctttgtctcg ataattttaa agtcttcgcc gatttgcaat
      781 tgtcagtaga ggaaattacg gatccgcagg tgaagctttt tcccaatcct gtttccgact
      841 tgctttcaat tcagtcgact gccggaattg agtcggtttt catttatgat gcagcaggaa
      901 aactgctgat gagcacagaa agcaattctt ccggattcgt ttctcttacg gtaaatcaat
      961 tgcctgcggg gttttatttt gccagagtta atacttccta tggtagtatt acccgacaat
     1021 tcattcgaca ttaaccaaat cctccaattt gcacattgaa ctaaatcagg ctttacctct
     1081 tcccatgcgg gatgagtcct tttcaccgga aagtctgtgg aagcgaagtg cgatactgga
     1141 atcaggaaat gatcacctca ttcttgctcc ttcaggtacc ggcaaaacct ctttactttc
     1201 ttttttattt ggaatgcgaa atgattatca gggatcaatt ctgatggata ataaggaggt
     1261 aaaaaccatt tctattgccg aatgggctct aatacgaaga gagagaatct ccattgtaat
     1321 gcaggatatg cgattaattc caaccctaac agttgaggat aatcttaatc tgaaaaacaa
     1381 tctgaaaaaa atgtatcgtg agaatgatat tcgcgatatg ctttcccggc ttggactaca
     1441 ggaaaaatgg aaacaaaagg caggtacact ttcgatcggt cagcaacaac gggtttccat
     1501 tgtgcgttca cttttgcagc cttatgagtt gttaatgctg gatgagccat tcagtcatat
     1561 cgatgaagaa aatatccgat tagcctccga attgatcgtt gaacaaaccc gaaaaaaggg
     1621 agctaatatt ctcatggttt cattgggatt ggattacaac atgaagttca accaacaaca
     1681 ccgattataa accatgatgc gcaaagtcct cctcaaaagt cagcgtcctt tgcaactcat
     1741 tgttgcgatg ctgggagcgc ttgcgggggt atttatgctg ttgtttagta ttcaggttta
     1801 tcttgatctt tctgcggccc tcaacgcgag ggaagatctt cttggttccg attatctggt
     1861 aatacaaaaa caggtaacta cacttgatag ttttaatcct gaaagccttg gttttggaac
     1921 gaatgaaatt gacgatatcc gtcatatgaa ctttgttgag gaagtaggtg tttttgaatc
     1981 atcaaggttc caggtctttg ctgccattac aggtttaccg ggttcaagag agatatatac
     2041 cgaagcttat ttcgaagctg tgccggatcg ttttttggat gtggtaacag gcgattggca
     2101 atggaaagaa ggtgatgaaa gagtcccggt tattttgccg gctgcttacc tcgatgcgtt
     2161 taattttggg ttcggtccaa gtcagggttt accaccatta accgaaaaag ctt
//
LOCUS       DAOMYG010000493         2212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_18981_length_2212_cov_8.262401, whole genome shotgun sequence.
ACCESSION   DAOMYG010000493 DAOMYG010000000
VERSION     DAOMYG010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18981_length_2212_cov_8.262401"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PK610_12950"
     CDS             complement(<1..716)
                     /locus_tag="PK610_12950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ36895.1"
                     /translation="MRTNIRHSGAFLLIIFFLVPFMSSAQKNEAELIKMANKLFDEGK
                     YTEAAPLFSQLLSNNAASPEYNYKYGTCLIFSDGEKTNGLKYLLFAAQSPGVNKDCWY
                     YLGRAYHLNYQFADAIRAYQKYISLATPKDVEKFQVNRHIEMCNNGRKLLRNITDLVV
                     IEKKIISEETFFRLYSMKDVGGQLIVVEALQSNLDKKNNHKIVIHNPKNSDVVFFASY
                     GSDGKNGQELTAVASYRLGPG"
     gene            complement(772..1419)
                     /gene="plsY"
                     /locus_tag="PK610_12955"
     CDS             complement(772..1419)
                     /gene="plsY"
                     /locus_tag="PK610_12955"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRJ36896.1"
                     /translation="MDVIFTPFNIIAAVVAYLMGSIPSAVWIGKIFYGTDIREHGSGN
                     AGATNTFRVLGKTAGLIVLSMDVSKGLIAITLLAWLNPYEAETLAQVNFKLAIGVIAV
                     LGHIFPIFAGFKGGKGVATLLGVVIALHWQAALMCIGIFLIIFLLTRYVSLGSMVTAI
                     SFPFLFFLVFRPVPLSLIYFAMVIAVLVLITHQKNIERLMRREENRMNIRLRKKG"
     gene            complement(1430..>2212)
                     /locus_tag="PK610_12960"
     CDS             complement(1430..>2212)
                     /locus_tag="PK610_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36897.1"
                     /translation="QIAKDEKLKENTLPGNLVAFAKEKCEIRGDGKMNFGVNTGQFKV
                     TPIGNITQKLGSGNPVMKVALMCDFPFSDDAMKKMADKIINYPDIQQFDYEKSNYEKA
                     LRELIGMEKADKVIADLSLHGEVKRFPDELEKSIVFADVTLKWIPEEGIWLSEGKLAI
                     SNINNKQIFKYVDGRVMIQKGRSFDQVSIVIKLDDSNWYFFTYAKELMEVTSSDSNFN
                     KIVQETKDDKRKYKGEKGQQDFEYRFNNNRRGRAQELLDRAN"
BASE COUNT          604 a          513 c          433 g          662 t
ORIGIN      
        1 cccggcccca agcgatagct ggccactgcc gtgagctcct ggccattttt tccgtcactt
       61 ccataactgg caaaaaacac gacatccgaa tttttcggat tatggattac gattttgtga
      121 ttgttctttt tatcgagatt gctctgcagt gcttcaacaa cgatgagttg accaccgaca
      181 tctttcatgc tgtagagacg gaaaaaagtt tcttccgaaa taattttctt ttcaatgaca
      241 accagatcgg taatgttgcg caacagtttt cgtccgttgt tacacatttc aatgtgacga
      301 ttcacctgaa atttctctac atcttttgga gttgcaagtg aaatgtattt ctgataagcg
      361 cgtattgcat cagcgaactg ataattgaga tggtacgcac gaccgagata ataccagcag
      421 tctttattta cacccggact ttgtgccgcg aagagcaggt attttagtcc gtttgttttt
      481 tctccatcgg agaagatcaa acatgttccg tatttgtaat tgtattcggg actggctgca
      541 ttattggata aaagttgtga gaataacgga gctgcttccg tgtacttccc ttcatcgaac
      601 aatttgttcg ccatcttaat caactctgct tcatttttct gagcagaaga catgaaggga
      661 accaagaaaa aaatgatgag caaaaatgcc ccgctatgtc ggatgttggt tctcaaatgc
      721 cagggttcct aaaggtctgt acaccaaacc ttaaagatag ttgaattatc cttagccttt
      781 tttgcgtagg cggatattca ttcggttctc ctccctgcgc atcaaacgct caatattttt
      841 ctggtgagta atcagtacaa gtacagctat taccattgcg aaatagatca gcgaaagagg
      901 taccggacga aaaaccagga agaacaaaaa cggaaaactt atcgccgtaa ccattgagcc
      961 cagagacaca tagcgtgtta aaaggaaaat aatgaggaat attccgatac acatcagagc
     1021 agcctgccag tgcaaagcaa tgacaactcc aagcaaggta gctactcctt ttccaccctt
     1081 aaatcctgca aaaattggga aaatatgtcc taaaacggcg atcacaccaa tagccagctt
     1141 aaagttgacc tgtgcaaggg tttccgcttc gtaaggattc aaccatgcca ataaagtaat
     1201 ggcaatgagt cctttagaaa catccatgga aagaacaatg agtcctgctg ttttgcccaa
     1261 tacccgaaat gtattggttg ctccggcatt tccgctgccg tgttcgcgga tatctgttcc
     1321 gtaaaagatt ttcccgatcc atactgccga cggaatagac cccatcaggt aggcaactac
     1381 cgcggcaata atgttaaagg gtgtaaaaat tacatccatt cttctgtttt tagttcgccc
     1441 gatccaacaa ctcctgcgcg cgaccgcgac ggttattgtt gaaacggtat tcgaaatcct
     1501 gttgaccttt ctcgccctta tacttccttt tgtcgtcttt tgtttcctga acgatcttat
     1561 tgaagttgct gtcggaagaa gttacctcca tcagctcctt cgcataagtg aagaagtacc
     1621 agtttgagtc gtctaattta ataacaatgc tgacttgatc gaagcttcgt cctttctgaa
     1681 tcatcacccg accatctaca tacttgaaaa tctgcttgtt atttatgtta ctaattgcaa
     1741 gcttaccctc agataaccat attccttcct ccggaatcca cttgagtgtg acatcagcga
     1801 aaacaatgga cttctccaat tcatccggaa aacgctttac ctcaccgtgt aagctaagat
     1861 cggcgatgac tttatcggcc ttttccattc cgatcagctc gcgaagtgct ttttcgtagt
     1921 tcgacttctc ataatcaaac tgttggatat cggggtaatt aattatttta tcggccattt
     1981 ttttcatggc atcatcgctg aaagggaaat cgcacatcag cgccaccttc attacaggat
     2041 tacccgagcc tagcttttga gtgatgtttc cgataggagt aactttgaat tggccggtat
     2101 tcactccaaa attcattttt ccatcaccgc gtatctcgca cttttctttt gcaaaggcaa
     2161 ccagatttcc gggtaaggta ttttctttca gcttctcatc ttttgcaatc tg
//
LOCUS       DAOMYG010000494         2211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_18999_length_2211_cov_10.413265, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000494 DAOMYG010000000
VERSION     DAOMYG010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18999_length_2211_cov_10.413265"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..447)
                     /locus_tag="PK610_12965"
     CDS             complement(103..447)
                     /locus_tag="PK610_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36898.1"
                     /translation="MFESRKKPLLSKGKFYNRVAKNIAFAFAIIAFSLLAGTLGYRHF
                     GSLGWIDAFYNASMILTGMGPVDPLPDEGKIFASVFALYGGFVVLSVFAIIVAPLVHR
                     FMHKFHFDDDNN"
     gene            complement(452..>2211)
                     /locus_tag="PK610_12970"
     CDS             complement(452..>2211)
                     /locus_tag="PK610_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRJ36899.1"
                     /translation="GEFLIRETEAKDIFIPEEWNEEQRMIAQTCRDFLEQEVFPKLDR
                     IDAMEEGLMPSLLDKAAELGMLGITVPEEYGGFGKDFVTGMLTTEVTGAGHSFAVALA
                     AHVGIGTLPILYYGNEAQKQQYIPNLASGSWKAAYCLTEPGSGSDANSGKTIAVLSAD
                     GKHYVLNGQKMWITNGGFADVFTVFAKIDNDENLSAFIVESKWEGVSLNPEEKKMGIK
                     GSSTRQVFFNNVKVPAENLLSERQNGFKIALNILNIGRIKLAGAALGGSKEAITKSVV
                     YANERQQFGRPISKYGAIRHKLGEQAIRTFAVESATYRCSQNVEDAIASLMAGGMDKE
                     KALLKGVEQFAAEAAILKIAGSEALDYVVDEGVQIYGGMGYSAEAPMERAYRDSRINR
                     IFEGTNEINRMLIVDMLMRKAMKGELDLMGPAMKVAQELMSIPDFGAGKEGLFAKEKK
                     YLSNYKKAVLMVAGSAAQKLMNDLAKEQEVLTNIADMVIDTYLAESTLLRVEKIISMK
                     GEAESAVYIDIAQVLLYDIADRINKSGKDAINSYAEGDELRMMLMGLKRFTKTEPLNT
                     KEARRRIAAKLIEANKYCF"
BASE COUNT          583 a          516 c          432 g          680 t
ORIGIN      
        1 gtggaaatca gtgaatccat aggatcaact tcattggtcg attctcctcc gcaggaaatg
       61 aatgcaaaac agattaaact tccgtaaaca aaggaggata ttttagttgt tgtcatcatc
      121 aaaatgaaat ttatgcataa aacgatgtac caatggagca acaataattg caaatacaga
      181 cagcacaaca aaacctccgt acaaggcaaa aacacttgca aagattttgc cttcatcggg
      241 taaaggatca accggaccca tgcccgtaag aatcatggat gcattgtaaa aagcatcaat
      301 ccatcccagc gatccgaagt gtcggtatcc cagtgtgccg gccagtaagg aaaatgcaat
      361 tattgcaaag gcaaacgcaa tgtttttcgc aacgcggttg tagaatttgc cttttgaaag
      421 taatggcttt ttccggcttt caaacatgta ttcagaaaca gtatttgttg gcctctatca
      481 gttttgcagc aatacgtcga cgtgcttcct tggtgtttag cggttctgtt tttgtgaatc
      541 gtttcaatcc cattagcatc atacgtagtt catcaccttc tgcatagctg ttgattgcat
      601 ctttaccgct cttgttaatg cgatcggcaa tatcgtaaag caatacctgt gcgatgtcga
      661 tataaacggc agattcagct tctcctttca tggagataat tttctcaaca cggagtagcg
      721 tgctttctgc cagataagtg tcgattacca tgtctgcaat attggtaagt acttcctgtt
      781 ctttagcaag gtcattcatc aatttttgtg cagcagaacc tgctaccatc aaaactgctt
      841 ttttataatt gctgaggtat ttcttttctt ttgcaaaaag tccttctttc ccggcaccga
      901 aatccggaat ggacattaat tcctgcgcaa ctttcattgc tggtcccatc agatcgagtt
      961 cgcctttcat tgctttacgc atcagcatat ccacaataag catgcggttg atctcattgg
     1021 ttccttcgaa gatcctgttg atgcgtgaat cacggtaagc cctttccatt ggggcttctg
     1081 ctgaatatcc cattccgccg taaatctgaa caccttcgtc aacaacataa tccagtgctt
     1141 cagatcctgc tattttcagg attgcagctt ctgcagcgaa ttgttctaca cctttcagca
     1201 aggctttttc tttatccatt ccacccgcca tcaaggatgc aatggcatct tctacattct
     1261 gcgaacaacg ataagttgca ctttctaccg caaaggtgcg gatagcctgt tctccgagtt
     1321 tatggcggat cgctccgtat ttggaaattg ggcgaccgaa ttgctgacgt tcgttggcgt
     1381 aaacaaccga tttggtaatt gcttcttttg atccacccag cgctgcacct gcgagtttga
     1441 tgcgaccgat attcagaatg ttcaatgcga tcttaaatcc attctggcgt tctgatagaa
     1501 ggttttcggc agggactttc acattattga aaaatacctg acgagtcgac gatcctttta
     1561 ttcccatttt cttttcttcg ggattgaggg aaacaccttc ccatttgctt tccacaatga
     1621 aagccgataa attttcgtcg ttatcgattt ttgcaaaaac ggtgaataca tctgcaaaac
     1681 caccattggt gatccacatt ttctgaccat tcaacacata gtgttttcca tcagcactca
     1741 atactgctat agtctttccc gaatttgcat ccgatccgct tccgggttca gttaggcaat
     1801 atgcagcttt ccatgaacca ctggcaagat tgggaatgta ttgttgtttt tgtgcttcat
     1861 ttccataata gagaatcggt aaagttccga tgccaacatg cgctgcaaga gcaacagcaa
     1921 acgaatgtcc tgcacctgta acttccgttg taagcatacc ggtaacgaaa tcttttccaa
     1981 atccgccata ttcttcaggg acagtaatgc ccagcattcc taattcggca gctttgtcaa
     2041 gaagcgaagg catcaatcct tcttccatag catcaatgcg atcgagtttg ggaaatactt
     2101 cctgctcgag aaaatcacga caggtttgcg caatcattcg ttgctcttcg ttccattctt
     2161 ctggaatgaa aatatctttt gcttctgttt cgcggatgag gaattctcct c
//
LOCUS       DAOMYG010000495         2210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_19021_length_2210_cov_5.649652, whole genome shotgun sequence.
ACCESSION   DAOMYG010000495 DAOMYG010000000
VERSION     DAOMYG010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19021_length_2210_cov_5.649652"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="PK610_12975"
     CDS             <1..1162
                     /locus_tag="PK610_12975"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ36900.1"
                     /translation="ANTTALGLDLYTGSINSRIHTIINDKLKNITGIQFQDMRNASYG
                     FEFLIPDYVQRSGGLYSLFTWEKTSKSTYTIGGRADLSGIDMKEHYQIIYDINQNVIA
                     TQYRSPNFSTNYFNWSFASGASWELAHEWNIKLHVGRSFRIPSAAELGANGVHHGSFR
                     HEMGDTSLRPEVGYQADLGLIHHDKHYIIKFTPFFNYFSNYLFLRPGAEFSPLPDAGQ
                     IYRYTQASVLHSGAELYAEYHIIDELHLEFAAEYVYNLNLDNYLPLPFTPPGSFSISP
                     EYTAKGKKALKTWFISSDIKYFLAQNRTDRNEKTTPGYLLFAASAGIHLQFKKWSCEL
                     LVRGNNLSNVSYLNHLSRYRILNMPEQGRNFSVVMKIPLSFSLAGKQTSNIN"
     gene            1220..1984
                     /locus_tag="PK610_12980"
     CDS             1220..1984
                     /locus_tag="PK610_12980"
                     /inference="COORDINATES: protein motif:HMM:NF026758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4625 domain-containing protein"
                     /protein_id="HRJ36901.1"
                     /translation="MKKSLPILLPFLGIVLFFSSCKKEDPDITKPYIGYLTINGISGD
                     VSASAGSTLSFVIGVNDDKELKQVKIDIHDAFDNHHHSPNVPWTSQTIYNVSGKTSTL
                     SPTLTIPANASSGPYDVILNCIDASGNEALFVLRKITITQNGQPVMNITAPDLGIELD
                     YAPGDTLHISGTIDEDNDLTEIHITLENETTSLLVYDEEFALPGTSDVQWDFSQLATL
                     SKWIILPTGTGHYHLKIKAVDNDANNTVIEQHVHVD"
BASE COUNT          674 a          462 c          426 g          648 t
ORIGIN      
        1 ggcaaataca acggcattgg gactcgattt gtatacggga agtataaatt cacggattca
       61 taccataata aatgataaac tcaaaaacat tacgggaatt caattccagg atatgcgaaa
      121 tgcttcttac ggatttgaat ttcttattcc cgattatgtt caacgttcag gaggattgta
      181 ttcgttgttt acctgggaaa aaacatcgaa aagtacttat accattggag gtagagccga
      241 tctttcaggt attgatatga aagagcatta tcagatcatc tatgatatca atcaaaatgt
      301 aattgcaaca caataccgct caccgaattt tagtacaaat tattttaact ggagttttgc
      361 aagcggagca tcatgggaat tagcgcatga atggaacatt aagcttcatg tcggacgctc
      421 attccgtata ccctcagcag cagaattggg tgcgaatggt gtacatcatg ggagttttcg
      481 tcatgaaatg ggcgacactt cacttcgtcc tgaggtgggg tatcaggcag atcttggact
      541 cattcatcac gataaacact acatcataaa attcacacct ttttttaatt atttcagcaa
      601 ttaccttttt cttcgacctg gtgctgagtt ttctcccttg ccggacgccg gacaaattta
      661 ccgttatact caagcaagtg tattgcattc gggtgcagag ttgtatgccg aatatcatat
      721 tatagatgaa ctccatctcg aatttgcagc agagtatgtt tacaatttaa atcttgataa
      781 ttacctccct ctgcccttta cgccccccgg cagtttctct atcagcccgg aatacaccgc
      841 gaaagggaaa aaagcattaa aaacctggtt tatatcctca gatatcaaat acttcctggc
      901 acaaaacaga accgacagaa acgagaaaac tacccctggt tatctgctat tcgctgcttc
      961 tgccggaata cacctgcaat tcaaaaagtg gtcctgcgaa ttgttggtgc gaggcaataa
     1021 cctttcaaat gtttcctatc tcaatcatct gagccgatac cgtatcctga atatgcctga
     1081 acaagggagg aatttttcag ttgttatgaa aatccccctt tccttttcac ttgccggaaa
     1141 acaaacatcc aacataaatt aaattttgca actgtgttgc atttatgaaa tattgtttat
     1201 atttgactaa acttacatta tgaaaaagtc actacccatt cttttgccat ttctcggcat
     1261 tgtgttattt ttttcaagtt gcaaaaagga agatcccgat atcactaaac catatatcgg
     1321 ctacctcacc ataaatggaa tttcgggtga tgtttctgct tcggcggggt caacacttag
     1381 ttttgtaatt ggggttaatg acgacaaaga actgaaacaa gtcaagatcg atattcacga
     1441 tgcattcgat aatcatcatc atagtccgaa tgttccttgg acgagccaaa caatttacaa
     1501 tgtatccggt aaaacgtcca cgctttcccc taccctcacc attcctgcga acgcatcgtc
     1561 cggaccgtat gatgtgattc tgaattgtat tgatgcctcc ggaaatgaag cgcttttcgt
     1621 attgcgcaaa atcaccatta cacaaaatgg tcagccggtt atgaacatta ctgctcccga
     1681 tttaggaatc gagttggatt atgcacccgg agacacattg cacatttcag gtacaatcga
     1741 cgaggataat gatttaacag aaattcatat tacgctcgaa aatgagacca cttcgcttct
     1801 ggtttatgac gaagaatttg cactaccggg aacatcagac gttcaatggg atttttcaca
     1861 actggctacc ttaagcaagt ggattattct tccgacaggt acaggacatt accatcttaa
     1921 aattaaggcg gtagataacg acgcgaacaa tacggttatt gaacagcatg tccacgtcga
     1981 ttagttcaaa ttatctcttg aaaaatgcat gattgaattt ggggtactga tttcttaatg
     2041 taaattagca tgcctatttc aatcaattaa accatgcaaa cagcagagcg cgtatccgat
     2101 gtagataaat ccgataattt cgtttaccaa cgaagtaaac tggcctattt ggaagcagca
     2161 aaacgagttc aaggcaaagt acttgaagtg ggttccggaa tgggttatgg
//
LOCUS       DAOMYG010000496         2206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_19080_length_2206_cov_10.607624, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000496 DAOMYG010000000
VERSION     DAOMYG010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19080_length_2206_cov_10.607624"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..2154
                     /locus_tag="PK610_12985"
     CDS             148..2154
                     /locus_tag="PK610_12985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ36902.1"
                     /translation="MKKIYAMIALSGLGVTAAQAQLRPATTKAPLAPASIVEANNPNS
                     NNLVGGERTPVWTEPFNIGTTSGSTNGPTWTTGNGVWTASGVNAAVWKHSHSGSTGQY
                     SASTPAFAGNSNANGWMLFDADAANAAQPFTNFTGTITSPIIDLTGLTSVTINFNQMY
                     RFCCASTHTLTLGVSGDGGTSWTDFSVTNPGVATNVNTANPDMVARNVSSALLNSSGV
                     PLPGIDMSQVRIRLTWDGAGASVSHYYWIVDDIEIGPAPDHDLEIYNTNFYGQTTFWD
                     TEHISYRQFPTNPANQFFPQMIIGDVFNVGALAQTNTALQGRILDAGSTAVFTGTDNA
                     GNGTLNPGSYQYDTIRNINISAYTAGTYNVNYEILYDNSALDARPANNTAPATPFLVG
                     GTEFGRDNNLYSGAGLYGGVDAGGGYKAYTMGNVFQITNNVTLKSVRVAFSATSSATT
                     AGSIVFPIVYEHDNSATAIDAALNPIYDGSGTSFEYEILPTDLSTTQLRWVDLRIPNL
                     NLQAGKSYVVAVGTYGAPDKVMIQNGNVNHPTFVTTFIYGDVGSAGLGWYWMTSTPKI
                     RMNFDPTIGVEENESVGGLTVMQNVPNPFTGETMINFSLENAAQVGFEVYDISGKMVY
                     TKNLGVLGAGSNSFRFDGAQLSAGVYYYTVSTPNGKVTKKMIIG"
BASE COUNT          578 a          511 c          465 g          652 t
ORIGIN      
        1 atcaaccgct tcccggtggt cacgggcacc gaccgggccg aagacgatgt ctacataatt
       61 ggcttaacgg cacaattgtt ttaggtaaag gtttagctgt tgttggcctt ttctgctata
      121 tttgaatatc tcataaacca acgaatcatg aagaagattt acgctatgat tgccctttca
      181 ggtttaggtg tgacagctgc ccaggcgcag cttcgtcctg caaccacgaa agctcccctt
      241 gcccccgctt caattgtaga agcaaacaac cctaactcca ataacctggt tggcggtgaa
      301 cgtaccccgg tttggacaga accttttaat attggaacta cttccggttc taccaacggc
      361 ccaacctgga ctacaggtaa tggtgtttgg actgcctcgg gagtgaatgc tgctgtatgg
      421 aaacattctc attcaggatc aacaggccag tattctgcta gtacacctgc atttgccggt
      481 aactccaatg caaacggttg gatgttgttt gatgccgatg ctgctaacgc tgctcagcca
      541 tttaccaatt tcacaggaac cattacttca ccaattattg atcttacagg attaacttct
      601 gtaactatta atttcaacca gatgtatcgt ttttgctgtg cttctacgca cactctaact
      661 cttggtgtat cgggtgatgg aggaacttca tggacagatt tttctgtgac taatcctggt
      721 gtggctacaa acgtaaatac tgcaaacccc gatatggttg cacgtaacgt ttcttctgcg
      781 ttattgaact cttcaggagt tccgcttccg ggaattgata tgagtcaggt tcgtattcgt
      841 cttacctggg atggtgccgg tgcgagcgtt tctcactatt actggattgt tgacgatatt
      901 gaaatcggtc ctgctcccga tcatgatctt gagatctaca acaccaattt ctatggtcag
      961 acaactttct gggacactga gcacatcagc taccgtcagt ttcctaccaa tcctgctaac
     1021 caattcttcc cgcaaatgat cattggtgat gtgtttaacg taggtgcact tgcacaaaca
     1081 aacactgccc ttcaaggtcg tatccttgat gcaggaagca ctgcggtttt cacaggaaca
     1141 gacaatgcag gtaatggaac attaaacccg ggttcttacc agtatgatac catccgtaac
     1201 attaacattt ctgcttatac tgccggaacg tacaatgtaa attatgaaat cctttacgat
     1261 aattcagcat tggatgcccg tcctgccaat aacactgctc ctgcaactcc tttccttgta
     1321 ggtggaacag aatttggtcg cgataacaat ctttattcag gagctggttt gtatggtggt
     1381 gttgatgccg gtggaggata caaagcctac actatgggta atgtgttcca gataacaaac
     1441 aatgtaacat tgaaatccgt tcgtgtagct ttctctgcca caagttcagc cacaactgca
     1501 ggttcaattg tattccctat cgtttacgaa cacgacaact ctgctacagc tattgatgct
     1561 gccctgaatc ctatttacga cggatcaggt acatctttcg aatatgagat tcttccaact
     1621 gacctttcta ccactcagct ccgttgggtt gacctccgta ttcctaactt aaatttacaa
     1681 gctggtaaga gctatgtagt tgctgttgga acttacggtg ctcctgataa agtgatgatc
     1741 cagaacggta atgttaacca cccgactttc gtaactactt tcatctacgg tgatgtagga
     1801 tctgcaggtc ttggttggta ctggatgaca tctactccaa aaattcgtat gaacttcgat
     1861 cctacaattg gtgttgaaga aaacgaatca gttggtggtc ttactgtaat gcaaaacgtt
     1921 ccaaacccat tcaccggtga aaccatgatc aacttctcac ttgagaatgc tgctcaggtt
     1981 ggatttgaag tgtatgacat ttctggtaaa atggtttaca caaaaaatct aggtgtactt
     2041 ggtgcaggaa gcaattcttt ccgtttcgac ggagctcagc tttctgcagg tgtgtattac
     2101 tacactgtaa gcactccaaa tggtaaagtg actaagaaaa tgatcatcgg ttaataccgg
     2161 actcatttct accgatacct caaaaggccg tcttcattgc gaagac
//
LOCUS       DAOMYG010000497         2193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_19245_length_2193_cov_7.821328, whole genome shotgun sequence.
ACCESSION   DAOMYG010000497 DAOMYG010000000
VERSION     DAOMYG010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19245_length_2193_cov_7.821328"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PK610_12990"
     CDS             <1..394
                     /locus_tag="PK610_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36903.1"
                     /translation="GWGPGTYQFYYASFQHSSELTIISTNFGDLGNAHSEYLGPLSEQ
                     GVPGLLLMLALILTVFYTGVKLYLDLPPGEWRLMVLVFLLGLLTYYIHGILNNYLDTD
                     KASIPFWGFTAVIVVVDVLFRRGELDTD"
     gene            complement(395..1150)
                     /locus_tag="PK610_12995"
     CDS             complement(395..1150)
                     /locus_tag="PK610_12995"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HRJ36904.1"
                     /translation="MITKKKQQSFLNKEGESLLKHIELYLRKRSMSDLHRFRVSCKHI
                     NMLVWLNRKITGDTSIQTTFLPVKNIFRISGQIREVQMHLHLLSPYRTKIQTLIPDLS
                     KEEKKLENNLLNQGLKGLKEIRSSLAALPDLTSTISGKDVKKLIEKEIRKIHSELDPA
                     MSAEDLHKVRKRIKTLVYLTDLGKTSDVDRTYLHRCEELIGEWHDINLLNAYLNSKKD
                     IPKSLLKEFDRKEQKLLNKIATEFHRFKRKVMR"
     gene            complement(1242..>2193)
                     /locus_tag="PK610_13000"
     CDS             complement(1242..>2193)
                     /locus_tag="PK610_13000"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRJ36905.1"
                     /translation="ESSSKKSLNTFDVAIPVFHKSNPLAFLLIGDLNEDELKISPTIK
                     HMPFVQTLTNIIVVAIENKRLAKEAIHQARMRKELELASEMQSMLLPSDLPQNNFVDI
                     AAKYLAHQEVGGDYYDFIRLNEDEFVFCMADVSGKGVGAALLMSNFQANLRAILKYTD
                     FPLKRLIEELNHGVMHSAKGEKFITFFIARYNQISRKLRYVNAGHNPPVLSDGKTIRH
                     LNAGCAGLGMVEHLPNIEEEEIFISPKSVVVCYTDGLVEIENDSGEDYSLERLSEVVR
                     RNSGISKTSLLNEDIYKDVNQFRGKSPFADDTALLSVRIN"
BASE COUNT          586 a          436 c          444 g          727 t
ORIGIN      
        1 cggttggggt ccgggtacct atcagtttta ttacgcgtct ttccaacatt catccgaact
       61 gacgatcatc tccacaaatt ttggtgattt aggaaatgcg catagtgagt atctgggacc
      121 gctttcagag cagggagttc ccggattgtt gttaatgctt gcgctgattc ttactgtttt
      181 ttataccggg gtaaaattat accttgatct tcctccggga gaatggcgcc tgatggttct
      241 ggtttttctt ttgggattgc tcacctacta cattcacggt attctgaata attatctcga
      301 taccgacaaa gcatccattc cattttgggg atttacagct gtaatagttg ttgtggatgt
      361 gctttttcgc aggggagaat tggatacgga ctaatcaacg catcaccttt ctcttgaacc
      421 gatggaattc agttgctatt ttgtttaaca gcttttgttc ttttctgtca aactccttga
      481 gcagcgattt tggaatatcc tttttcgaat tcaaataagc attcaataag tttatatcgt
      541 gccattcgcc aatgagttct tcgcaacggt gaaggtaggt tctgtcaaca tccgatgttt
      601 ttccgaggtc agtgaggtac accaatgtct tgatgcgttt tcttaccttg tgaaggtcct
      661 ctgcgctcat tgcaggatca agttccgaat ggatctttcg gatctctttt tcaattaatt
      721 tcttgacatc tttcccggaa atagtgcttg ttaaatcggg taatgccgcc aatgaacttc
      781 gtatttcctt cagccctttt aaaccctgat tgagcagatt attctcgagt tttttttctt
      841 ctttactgag gtcaggaata agtgtttgga ttttcgtccg gtagggagaa agcaaatgta
      901 aatgcatctg aacttccctg atttgaccag agatcctaaa aatatttttt actggaagaa
      961 atgtggtttg aatgcttgta tcccctgtga tttttcggtt aagccaaacc aacatattga
     1021 tgtgcttgca cgaaaccctg aaacgatgaa gatcactcat gcttcttttt cgcaggtata
     1081 attcaatatg cttgagtaag gattcccctt ctttgttaag gaaggattgt tgtttttttt
     1141 tggtgatcat gatccgataa tgggtttttc gaaaacaaat ctcaggaaga aatgattatt
     1201 tggtgaaatg tgattcaccg taaattaaca tttcatttcg gtcagttaat tcgaacagac
     1261 aatagcgcag tatcatcggc aaacggactt tttcctctga actgattcac atccttatag
     1321 atatcttcat tgagaaggga agttttgctt attccgctat tgcgccgtac aacttccgat
     1381 agtctttcga gtgaataatc ctcaccgcta tcattttcaa tttccaccaa accatcggta
     1441 taacaaacaa caactgattt tggagaaatg aagatctctt cttcttcaat attgggtaaa
     1501 tgttccacca tgcctaatcc tgcacagcct gcattcaaat ggcgaatcgt ttttccgtca
     1561 cttaataccg gaggattgtg accagcgttt acatagcgta gtttacgact gatctgattg
     1621 tatcgggcga taaaaaaagt aatgaatttt tctccctttg cgctatgcat tacgccgtga
     1681 ttcaattctt caattaaacg cttgagcgga aaatcggtgt atttgagaat tgcacgaaga
     1741 ttggcctgga aattcgacat cagcaaggct gctccaacac cttttccgga gacatcagcc
     1801 atgcagaaaa caaactcgtc ttcattcagt cggataaaat cataataatc gccaccaact
     1861 tcctgatgag cgaggtattt tgcagcgata tcaacaaaat tattttgcgg aagatcagaa
     1921 ggtaaaagca tggattgcat ttcggacgca agctcaagct cttttcgcat ccgggcctga
     1981 tgaatggctt cttttgccag acgtttattt tcaatcgcaa caacaataat attggtaagc
     2041 gtttgcacaa atggcatgtg cttaatggtt ggagagatct ttaattcatc ttcattcaga
     2101 tcaccaatta gcaaaaaggc caagggattt gatttgtgaa atacaggaat tgcaacgtca
     2161 aatgtattca gggatttttt agatgaagat tca
//
LOCUS       DAOMYG010000498         2183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_19353_length_2183_cov_8.378289, whole genome shotgun sequence.
ACCESSION   DAOMYG010000498 DAOMYG010000000
VERSION     DAOMYG010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19353_length_2183_cov_8.378289"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2183)
                     /locus_tag="PK610_13005"
     CDS             complement(<1..>2183)
                     /locus_tag="PK610_13005"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRJ36906.1"
                     /translation="GSVLYIAAHPDDENTRLISYLTKERKFEVAYLSLTRGDGGQNLI
                     GDEQGEDLGIIRTQELLEARKIDGAIQFFTRAFDFGYSKSPQETFMKWNKEEVLADIV
                     FVIRLFKPDIIICRFPTTGEGGHGHHTASAILAEEAFDAAADPNRFPEQLAAVSAWKT
                     KRLLWNTFNFGTNNTTAEDQLKIDAGGFNPLSGKSNGEIAAEARSMHRSQAFGTERRR
                     GEQFEYFKTLKGSPASNDIMDGIATDWTRFKATSSINAIIEKIEKKFRHENPELIIPD
                     LIRLYQVLNKYSSNKEPELQHWVSRKTNEINTLIQNCAGLFIEFTTDKPVYSIYDTVN
                     ITIKAINRSKTKIQFASLFSVFGKTINQFHEIHDQILEPNQLKTATIKMVIPETYLPS
                     NPYQYRYSNENQTRFRWPMPIKPQYKEESLVSLGIKINGQLIVVQRDLHYKKVDPSFG
                     EIYQPFVIAPEISLNFTEEQYIFNGSTSRKIRIRAKAFRPIKKALVSLEYPNGWQVTE
                     KEIELSFSATGEEKEIEFEITPSTSSTSGKQQLKAFIDINGKRNSWMEHFIQYDHIEN
                     QHWFTESVAGITATNIKTNTKSIGYLAGAGDKIPGILRQLGYEVTLLNEKLLNSLNLS
                     QFDAIVTGVRAFNTESYLKHINKKLFAYAETGGILVIQYNTSTSLVTDQLGPYPFKLS
                     RDRVTEEDSPVELLQKDHPLMRYPNYIAPEDFEGWVQERGLYFPG"
BASE COUNT          570 a          406 c          461 g          746 t
ORIGIN      
        1 accgggaaaa tacaatcctc tttcctgtac ccagccttca aaatcttctg gagcgatata
       61 atttggataa cgcatcaggg gatgatcttt ttgcaaaagt tcaaccggag aatcttcttc
      121 ggtgacgcga tcacgggaaa gtttgaaagg ataggggcca agctgatcgg ttacaagcga
      181 cgtacttgta ttgtattgga taacgagtat gcccccggtt tctgcgtagg caaaaagttt
      241 tttattgatg tgtttaagat aggattcagt attaaacgca cgaacaccgg taacgatcgc
      301 atcgaattga gacagattta atgaattcag aagtttctca ttaagcagcg ttacttcata
      361 gccgagttgt cgcaaaatgc cggggatttt gtcgcctgca cctgccaggt atccgatcga
      421 ttttgtattt gttttaatgt ttgttgccgt gattcctgct actgattcag taaaccaatg
      481 ctgattttcg atatggtcat attggataaa gtgttccatc catgaattac gtttgccgtt
      541 aatatcaata aatgctttca gttgttgttt tcctgaagtt gaagatgtag agggagtgat
      601 ttcaaattcg atttcctttt cttctcctgt ggcagagaag gataattcaa tttctttttc
      661 cgtaacctgc cagccgtttg ggtattcgag tgaaacaaga gcctttttaa ttggtcgaaa
      721 tgctttggct cgtatccgta tttttcttga tgtgctgccg ttgaaaatat attgttcttc
      781 tgtgaaattc aaactgatct ccggtgcgat aacaaaaggt tggtagattt caccaaacga
      841 aggatcaact tttttataat gtaaatcacg ttgaacaaca attaactgac cgtttatttt
      901 aataccaagt gaaaccaatg attcttcctt gtattgtggt ttaatgggca tgggccagcg
      961 aaacctggtt tgattttcgt ttgaatatct gtattgatag ggattggatg gtaaataagt
     1021 ttccggtatt accattttaa ttgttgctgt ttttaattga ttaggctcaa gaatttgatc
     1081 atggatttca tggaactgat taatggtttt tccaaaaacg gaaaacaagg atgcaaattg
     1141 aattttggtc ttcgatctgt tgatcgcctt tatggtgatg ttgactgtgt catatatgga
     1201 atagaccggt ttatcagtgg tgaattcaat gaatagtcct gcgcaatttt gaatcagcgt
     1261 atttatttca ttcgttttcc gactaaccca atgctgaagt tcaggttctt tattggaaga
     1321 atatttattc aaaacctggt ataaacggat taaatcagga atgatcaatt cagggttttc
     1381 gtgcctgaat tttttttcaa ttttttcaat tatagcattt attgaagaag ttgctttgaa
     1441 tcgtgtccaa tcggtagcaa tcccgtccat gatatcgttg ctcgccggac ttcctttcaa
     1501 tgttttgaaa tattcgaatt gttcacctct tcttcgttct gttccaaatg cctgactgcg
     1561 atgcattgac cgggcttctg cagcgatttc accgttggat tttccagata ggggattaaa
     1621 acctccggca tcaattttaa gttggtcttc agctgttgta ttgttggttc caaaattaaa
     1681 agtattccaa agcaatcgtt tggttttcca agcgctaact gctgcaagtt gttcaggaaa
     1741 tctgttgggg tctgctgcag cgtcaaacgc ttcctctgca agtattgcag atgctgtatg
     1801 gtgcccatgt ccgccttcac ctgttgttgg aaatctgcaa atgattatat cgggtttaaa
     1861 aagacggatg acaaatacga tatccgccaa aacttcttct ttgttccatt tcataaatgt
     1921 ttcctgtggt gatttggaat aaccaaagtc gaatgcgcgg gtaaagaatt gtatagcgcc
     1981 gtcaattttc cttgcctcga gtaattcctg cgttcggatt attcccaggt cttcaccttg
     2041 ttcgtcgcca atgagatttt gtccgccatc tcctctggta agagataaat acgcaacctc
     2101 aaatttcctt tctttggtga ggtaggaaat taatcgagtg ttttcatcgt ccggatgagc
     2161 agcaatatac agcaccgacc caa
//
LOCUS       DAOMYG010000499         2181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_19383_length_2181_cov_8.834431, whole genome shotgun sequence.
ACCESSION   DAOMYG010000499 DAOMYG010000000
VERSION     DAOMYG010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19383_length_2181_cov_8.834431"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1522
                     /locus_tag="PK610_13010"
     CDS             152..1522
                     /locus_tag="PK610_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36907.1"
                     /translation="MPRLLILLMLFPFITTCFAENLVKNPSFEEGLPEKTIRKRNGAG
                     TDAIYPTSWTSPSLGTPDLFNSPYSSGGKLSCAIARTGSGVMGMVMAESKNLENYYGG
                     SYKEYIQGELSSPLEAGKAYRVRFWIAHDRTSRFTAKNIGACFSNDPIRTDNHLHIDR
                     EPHVKMQDSMLVTAVMGWTKVEGVFVAKGGERFITIGSFGKDFMIPISSQGMAPLVTK
                     GHTHRNAYYYIDDITVESAEELRRHEKSELSSLIFLFDPSHAMPDRAMLEKWKKSFLA
                     LENVLPPETLISVFLAGNQARSLCEREPVPIAMQKINTSIEALIPSQGTESSFDKVFR
                     SVVQPILSKPKDNEHIIALFQDKQKMTGDTRSLMKKCYDKYDITFSVVQWGGKEINDF
                     KGLINYCEGQYFMTESDPQFFMRDAALLGVNHKNVVHYSKDRSGKMLYYIGAGALVAL
                     IIGSAL"
     gene            1519..>2181
                     /locus_tag="PK610_13015"
     CDS             1519..>2181
                     /locus_tag="PK610_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36908.1"
                     /translation="MRWLLLFFVLIVAGDVFAQENLVINPGMEDSVKRDAIHMYNTSL
                     RVGLPLAPGWVTPTRSTSDFYNSHESTIKGSPIVVAHRGSGRIGFIAGAKTRSSGQLY
                     KEYAMGKLSRPLEANKNYCVRFYIALDGSCKYANDSIGVYFSTQPIREENSYTLQNLR
                     PHIVMPPDSFIVSKDGWVEVSGYYPASGGEQYITIGCFSNKDRVKLKSIGERRTERFH
                     FSK"
BASE COUNT          605 a          487 c          477 g          612 t
ORIGIN      
        1 aaccggattg tccatccctc cttccctcca tctgtgaaat cccctcgcct tgtacccacc
       61 gaaatcttcc actaagccct ctgaattacc cttccctaca tccctccttt ggtgacaccc
      121 cacaatcccc caaaatccgt atttttatcc catgccacgc ctgctcatcc ttctcatgct
      181 gttcccgttc atcaccacct gttttgctga aaacctggtg aagaatccct cattcgaaga
      241 aggacttccg gaaaaaacga ttcgcaagcg aaatggtgca ggtacggatg cgatttatcc
      301 aacgagctgg acatccccaa gtcttggcac tcccgatctt ttcaattcgc cctacagctc
      361 cggaggaaaa ctctcttgcg ccattgcccg taccggttcc ggagtaatgg gaatggtaat
      421 ggccgaaagt aaaaaccttg aaaattatta cggtggttcc tacaaagaat acattcaggg
      481 agaattatcc tctccactcg aagcaggaaa agcctaccgt gtacgttttt ggattgccca
      541 cgacagaacc agtcgtttta ctgcaaagaa tatcggtgcc tgttttagca atgatcccat
      601 tcgtacagac aatcatttgc atattgatcg cgagcctcat gtaaaaatgc aggatagtat
      661 gttggtcaca gccgtgatgg gttggacaaa agtagaaggt gtatttgttg caaagggtgg
      721 agaacggttt attaccattg gtagtttcgg taaggatttt atgattccca tctcttcgca
      781 aggaatggct cccttggtaa ccaaaggaca tacacaccgc aatgcctatt attacatcga
      841 cgatatcact gtagaatcag cagaagaact tcgccggcat gaaaaatccg aactcagttc
      901 attgatattt ttattcgatc cttcacatgc catgcctgat agggctatgc tcgagaaatg
      961 gaagaagtca tttcttgccc tggaaaacgt tttaccaccc gaaacgctca tttcggtctt
     1021 cctcgccgga aatcaggcac gttctttgtg tgagagagag cctgtaccca tcgccatgca
     1081 aaagatcaat actagcatcg aagcattgat tccctctcag ggaaccgaaa gctcgttcga
     1141 taaagttttt cgttcggtag tgcaaccgat actttccaaa ccaaaagaca atgaacacat
     1201 cattgcactg tttcaggaca agcaaaagat gacaggtgat acacgaagtt tgatgaagaa
     1261 gtgttacgat aaatacgata ttactttttc tgttgtgcaa tggggaggca aggagatcaa
     1321 tgattttaag ggattgatta attattgcga aggacagtat ttcatgaccg aaagtgatcc
     1381 gcaatttttt atgcgcgatg cagctttgct gggagtaaat cataagaacg tcgtgcatta
     1441 ttccaaagat cgttccggta agatgttgta ttacattggc gccggtgcat tggttgcatt
     1501 aattattgga agtgcattat gaggtggctg ttattgtttt tcgttttgat tgttgccggt
     1561 gatgttttcg ctcaggaaaa tctggtgatc aatcccggaa tggaagattc cgttaagcgc
     1621 gatgccatcc atatgtacaa taccagtttg cgtgttggac ttccactcgc accgggttgg
     1681 gttactccca cacgatctac ttctgatttt tacaattctc atgagtccac catcaaagga
     1741 tcacccatcg ttgttgcaca cagaggctcc gggagaattg gatttattgc aggagcaaaa
     1801 acgcggtcaa gcggacaact ttacaaggaa tatgcaatgg gaaaattatc gcgcccattg
     1861 gaagccaata aaaattactg tgttcggttt tatatcgcac ttgatggttc gtgtaagtat
     1921 gcaaacgatt ccattggtgt ttatttctcc acgcaaccca taagggaaga gaattcttac
     1981 acattgcaga atttgcgtcc acacattgta atgccaccgg atagttttat tgtgagtaaa
     2041 gacggttggg tagaagtatc gggttattat ccggcaagtg gaggagagca gtacattacg
     2101 attggttgtt tctccaacaa agacagagta aaacttaaat ctattggtga acgcagaaca
     2161 gagcggtttc atttttcgaa a
//
LOCUS       DAOMYG010000500         2177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_19446_length_2177_cov_11.055137, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000500 DAOMYG010000000
VERSION     DAOMYG010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19446_length_2177_cov_11.055137"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PK610_13020"
     CDS             <1..215
                     /locus_tag="PK610_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36909.1"
                     /translation="DVDRGYGQSSAREVKKILEESIEGENKRKPLTDDELAIVLKEKG
                     YNIARRTVAKYREQLNIPVARLRKEL"
     gene            218..844
                     /locus_tag="PK610_13025"
     CDS             218..844
                     /locus_tag="PK610_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36910.1"
                     /translation="MPQRIAHLLSVFFHPIFVPLFALFLIFHSDTYLAYHSSPEARRM
                     LYIIVFILTVAMPGLSTYILVKNQMVSNITLPEKRDRTAPYLITAFYYALLYYLLRRI
                     PHIPPPLLSMVLGAICTLVLSTIINFRFRLSAHTAGISGIIGIYAALSGSAVLLPERW
                     VICSLILLAGIIGTARLLLNAHRPHEIYLGAAVGFLSEYVFIRYQIFL"
     gene            complement(851..>2177)
                     /locus_tag="PK610_13030"
     CDS             complement(851..>2177)
                     /locus_tag="PK610_13030"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRJ36911.1"
                     /translation="MVAKGFNDNQDTKTAEEKANATEREMVKRSSAIGVLQAEKNVHW
                     STIQSSLKKNEAAIEFVAFLDKYHDLTIPRYAALVIRPGMKQPEMIALCDAKQIESLL
                     RNSSATSLFAVQQVYGTLQKKNSKLYALIWKPLDAVLKGVTRVYYAPDGLLHKISFAA
                     LSDDQGNYLSDNYQLYPVSNTAVLAQPKDAVIRLSEIHLYGGVEYAYGNAPKVWEYLP
                     GTRLEADKISELLKRKGIVTEYYFGNEGTELRFRERSSAGLVLHVSTHGFFFPDPQSA
                     QETESKEVQTEVSFRGTSTEGRNRFVINPNPMMRSGLVFAGANRVWAQDEETEADQDG
                     VLTALEISTLDLSKVQLVVMSACETGLGDVRGSEGVFGLQRSLKMSGVRYIMMSLWQV
                     PDKETAEFMLLFYSALLKSKDVRQSFESAQKVMRKKYDPFFWAAFVLVE"
BASE COUNT          577 a          562 c          412 g          626 t
ORIGIN      
        1 gcgacgtcga ccggggttat ggtcagtcct cggcccggga agtaaaaaag attttagaag
       61 aaagcatcga aggagagaac aagagaaaac ctcttaccga tgatgaactt gcaattgttc
      121 tcaaagaaaa aggctacaat attgcgcgac gcacagttgc caaataccgt gaacaactga
      181 atattcctgt tgcccgtctt cgcaaggaac tttgatcatg ccgcaacgaa ttgcccacct
      241 cctctcggta ttcttccatc cgatttttgt cccgctcttc gcgctattcc tgatctttca
      301 ttccgacact tatcttgctt accactccag tcctgaagca aggagaatgc tttacattat
      361 tgtttttatt ctcacagttg caatgccagg cttatccact tacattctgg tcaaaaacca
      421 aatggtaagt aatattactc ttcccgaaaa aagagaccga accgctccat atctgatcac
      481 tgcattttat tacgcattgc tgtattacct gcttcgaaga attccccata ttcctcctcc
      541 actactcagc atggtgctgg gcgctatttg caccttggtt ctttccacca tcattaattt
      601 tcgcttccgt ctgagcgcac acactgcggg aatttccgga atcatcggga tttatgctgc
      661 gctttcagga agtgctgttt tattacccga acgatgggtc atttgttcct tgatccttct
      721 tgctggaatt attggtacgg cgcgcttact gctgaacgca caccgtccac acgaaattta
      781 cctcggtgca gctgtgggat tcctgagtga atatgttttt atccgctacc agattttcct
      841 ctgaatcgct ttattccacc agcacaaaag cagcccagaa aaatggatcg tattttttgc
      901 gcatgacctt ctgcgccgat tcaaacgact gacgtacatc tttgcttttc aataaagctg
      961 aatagaacaa caacatgaac tccgctgttt ctttatccgg aacctgccaa agactcatca
     1021 tgatataacg cacgcccgac atcttcagcg atcgctgcaa tccgaacacc ccttccgatc
     1081 cgcgcacatc tccaagacct gtttcgcagg ccgacatcac caccaattgc actttggaaa
     1141 gatccagtgt agaaatttcc aatgcggtta atacgccatc ctgatcggct tctgtttcct
     1201 cgtcttgtgc ccataccctg tttgctcctg cgaaaacaag tcctgaacgc atcataggat
     1261 ttggattgat cacaaaacga tttcttcctt cagtagatgt cccgcgaaac gaaacttccg
     1321 tttgaacttc tttcgactcc gtttcctgcg cgctttgcgg atcgggaaag aaaaatccat
     1381 gagtggaaac atgcaatacc aatcccgcag aagaccgctc gcgaaaacga agttctgtgc
     1441 cttcattacc gaaataatac tccgtaacaa ttccctttcg tttaagaagc tcagaaattt
     1501 tatccgcttc aagacgcgtt ccggggaggt attcccaaac cttgggagca tttccatatg
     1561 catattccac cccaccgtac aaatgtattt ccgacaacct gataactgca tcttttggtt
     1621 gagccagaac agctgtattg gagacaggat acaattggta attatccgat aaataatttc
     1681 cctgatcatc actcaatgca gcaaatgaaa ttttatgcaa caatccatcc ggagcgtaat
     1741 aaacccttgt aacgcccttc aaaaccgcat caagaggttt ccagatcaat gcatacaact
     1801 tgctgttttt cttttgtagg gtgccgtaaa cctgttgcac ggcgaacaag gatgttgctg
     1861 aactgtttcg aagaagtgat tctatttgct ttgcatcaca caaagcaatc atctccggct
     1921 gtttcatgcc aggacgaatg accaaagcag cataacgagg tatcgtcaga tcatggtact
     1981 tatccagaaa agcaacaaat tcaattgcag cttcattctt tttaagagag gactgaatag
     2041 tgctccaatg cacatttttt tcagcctgca gaacaccgat agctgaagat cgttttacca
     2101 tttcccgttc tgtagcatta gctttctctt ccgccgtttt ggtgtcctga ttgtcgttaa
     2161 atcctttagc taccatt
//
LOCUS       DAOMYG010000501         2173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_19504_length_2173_cov_11.336166, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000501 DAOMYG010000000
VERSION     DAOMYG010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19504_length_2173_cov_11.336166"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..558)
                     /locus_tag="PK610_13035"
     CDS             complement(157..558)
                     /locus_tag="PK610_13035"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRJ36912.1"
                     /translation="MHTHETELRVRYGETDQMGYCYYGNYAEYYEVGRVEALRDLGLS
                     YRSLEESGVMLPVLEFKIKYFKPALYDDLLKLKTTIAEISGARIRFVYEMYNESGQKL
                     NEGETTLVFINKNTNKPCPPPESLVGKLKKD"
     gene            complement(656..>2173)
                     /gene="sppA"
                     /locus_tag="PK610_13040"
     CDS             complement(656..>2173)
                     /gene="sppA"
                     /locus_tag="PK610_13040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRJ36913.1"
                     /translation="GVFLDVSMIPAGMATVEEIRNGLKKFKESGKFILAYSEIYTHKS
                     YYLASVADEIHLYPQGIVQHTGLTAEIMFMKGLIEKLELDLTIIRGSNNQFKSAVEPY
                     MLDKMSDANRKQTEKYLFSLWNHMLEGISQERKIEVSKLNQLADSLVLQTSDAALEER
                     FIDRISYRDEIYAELRKRLSIEEKDELNLVSLGTYAKDKRLNKTRSEKNSEKNKAKVA
                     VIYAVGDIIDGKGDDNSIGSVNLSEDIRAARLDTTVKAIVLRVNSPGGSALASDVIWR
                     EVVLAGKVKPFVVSMGDVAASGGYYISCAAHKIFAQPNTITGSIGVFGVLPNTERMLK
                     SKLGVTYDRVKTNRYSDIGSMSRAMDEMEYQIIQKGVDDIYADFINKVAEGRQSAGLT
                     PAKVDSIGQGRVWAGTDALAIGLVDELGGLETAISDAAKRANLTEGEYILAEYPIVKD
                     PIEELVNRLKNEAKVELINANFALSPEMMLYLYNVKEILRRKGVAALLPYHLEIH"
BASE COUNT          550 a          506 c          444 g          673 t
ORIGIN      
        1 atttaatttc tgtggcatgc aggctaaccg taaatgcaaa tagggagaaa aagagaacaa
       61 tgcgtttcat gggtatgttg ttttgagtgt agacaatgat ttaacgtaag agttgcttgc
      121 aattcccgtg cctcattgtt aaaaaaagta aaatgctcaa tcttttttga gtttgcccac
      181 tagcgattcg ggtgggggac aaggcttatt cgtgtttttg ttgatgaaga ccagcgtcgt
      241 ttcgccttcg tttaattttt gaccactctc attgtacatt tcgtacacaa agcgaatgcg
      301 tgctccggat atttcggcga tggtcgtttt tagtttcaac agatcatcgt acaaagcagg
      361 tttaaagtat ttgatcttga attccagaac gggcaacatc actccggatt cttccaacga
      421 tcggtagctt aatcccagat cacgcaaggc ttcaaccctt ccaacttcat agtattcagc
      481 atagttgccg taataacaat agcccatttg atcggtttct ccgtagcgga ctctcagttc
      541 tgtttcgtgt gtgtgcatag gtgaaaataa ccttttatta aacgtaaaaa ccccgacgag
      601 aacgtcgggg ttttagctca ggacttaaat ccgttctaaa catcatggca aaggttcagt
      661 ggatctctaa gtggtatggt agcaaggctg ccacaccctt gcgcctgaga atttctttaa
      721 cgttgtaaag atacaacatc atttcggggc ttaaggcaaa atttgcgtta attaattcaa
      781 cttttgcctc attttttaaa cggttaacca attcctcaat tgggtcctta actatcggat
      841 attcagcgag tatgtactct ccttctgtca ggttggcacg cttggcagca tcggaaattg
      901 ctgtttccaa accgcccagc tcgtccacca gcccaatcgc caaagcatcg gtcccggccc
      961 agactcttcc ctggccaatg gagtcgactt ttgcgggtgt taatcctgct gactggcgac
     1021 cttcggctac cttattaata aagtcagcat agatgtcatc aacccctttt tggatgatct
     1081 ggtactccat ttcgtccatg gctctgctca tcgatccgat atcggagtag cgattggttt
     1141 taaccctgtc gtaggttacg cccaattttg atttgagcat gcgctccgta ttgggcaata
     1201 ccccgaatac accaatcgat ccggtaatgg tattgggttg tgcgaatatc ttatgggcag
     1261 cacagctgat gtaataacca ccggatgcag caacgtcgcc catggaaaca acaaagggtt
     1321 ttacttttcc tgccaatacc acttctctcc agatcacatc tgaagccaga gcagatcctc
     1381 cgggtgagtt aacgcgtagt acaatcgcct ttacggttgt gtccaaacgc gcagcgcgaa
     1441 tgtcttccga caaattcacc gaaccaatcg aattatcatc gcctttacca tcgatgatgt
     1501 ctcccaccgc ataaatcact gcaactttcg ctttgttctt ttcactgttt ttttctgaac
     1561 gtgttttatt caggcgtttg tcttttgcgt aagttcccaa tgaaaccaga ttcagttcat
     1621 ctttttcttc aatgctcaaa cgcttgcgca attctgcata aatctcatca cggtatgaaa
     1681 tacgatcaat gaaacgctct tccaatgctg cgtctgaagt ttgcaataca agtgaatcgg
     1741 ccagctgatt gagtttgctc acttcaattt tccgctcttg agaaatacct tcgagcatgt
     1801 ggttccaaag tgagaacaaa tatttttcgg tctgttttcg gttggcatcg ctcatcttat
     1861 ccaacatgta gggttctact gcactcttaa attgattgtt cgatccgcga atgatggtca
     1921 ggtctagttc cagtttttca atcagtcctt tcatgaacat gatctctgct gttaatcctg
     1981 tatgttgtac aattccctgc ggatacaaat gaatttcatc ggcaacactc gccaggtaat
     2041 aggatttgtg tgtgtagatc tccgaatagg ccagaatgaa ttttccggat tccttaaatt
     2101 ttttcaaacc gtttctgatt tcttccactg tggccatacc ggcgggaatc atcgatacat
     2161 caagaaaaac acc
//
LOCUS       DAOMYG010000502         2166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_19602_length_2166_cov_9.039318, whole genome shotgun sequence.
ACCESSION   DAOMYG010000502 DAOMYG010000000
VERSION     DAOMYG010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19602_length_2166_cov_9.039318"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="PK610_13045"
     CDS             complement(<1..1203)
                     /locus_tag="PK610_13045"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ36914.1"
                     /translation="MNRLNGFEKQIKDGLENFEMPFDDAAWSAMEQKLDASAGDAFES
                     MLRKKVENHESPFDATQWTRLERSLPKKGISYRPWVIAASLVGVIGGSILIYNLNTES
                     ADENKTEIGRLEKNIISENSIIDHAIDNKETSDNSKVVNGMNVETTSTSKTSSSVKEN
                     NVLAEEVKSNTEKSGTTEKESNKENGRTETSKTTVESNTKENNSVSKEIVTATEITLP
                     QPLFSISNDEICTGQEVSFTASNIPVAANCVWEVNHNGAAIKTAKQFTYVFADPGSYT
                     ISLHFQNEKGQSAATTKTVVVKPSPQLTLQMEKVNENGRPFYHFSTRSSDVSSVNWTF
                     HDGTVSTEAEPVKFYRHKGDYKLTVDASSANGCQVRIVKSVHVDEDYNLFAPNAFSPN
                     GDNLNNVFI"
     gene            complement(1200..1799)
                     /locus_tag="PK610_13050"
     CDS             complement(1200..1799)
                     /locus_tag="PK610_13050"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ36915.1"
                     /translation="MSTRPMKLTPEQLEVAIRGCMDGDRRCQEQVFKEFFGKMMSVCM
                     RYTRDKDTAQDMLQDAFIKTFDKIGDFNFSGSFEGWLRRIVVNTAIDHIRRNKQMLTI
                     DDDSLIKDEDKDSVGAFQEENEDLAPYANVSPEMIMEAMQKLSPAYQAVFNLYVFENY
                     THKDIADALQISVGTSKSNFAKAKMRLKILLQKQLSIKE"
     gene            2055..>2166
                     /locus_tag="PK610_13055"
     CDS             2055..>2166
                     /locus_tag="PK610_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRJ36916.1"
                     /translation="MKITVVGAGAVGATCADNIIRKELAEEVVLLDIKEGL"
BASE COUNT          599 a          460 c          420 g          687 t
ORIGIN      
        1 aataaacaca ttgtttaaat tatcaccatt tggtgaaaat gcatttggag cgaatagatt
       61 ataatcttcg tcgacatgca cagatttcac aattcgcacc tggcaaccgt tagcggatga
      121 agcatccact gttaatttgt aatcaccttt gtgacgataa aatttcaccg gctcagcttc
      181 tgttgaaaca gttccgtcgt ggaaagtcca gtttacagat gaaacatcag aagaacgcgt
      241 ggaaaaatga tagaatggtc tgccattttc atttactttt tccatttgta aagtaagctg
      301 cggacttggt tttacaacaa ctgtttttgt ggttgcggct gactgtcctt tttcgttctg
      361 gaaatgcaag cttattgtat aagacccggg atcagcaaat acataggtaa attgctttgc
      421 ggtttttata gctgcaccgt tatggttgac ttcccaaaca caattagcag ctacaggaat
      481 attagatgcg gtaaacgaaa cttcctgccc ggtacaaatt tcatcattcg aaatactgaa
      541 caacggttgc gggagagtaa tttctgttgc ggttacaatt tcttttgaaa ctgaattatt
      601 ttcttttgtg ttactttcta cagtagtttt tgaagtctca gtgcgaccgt tttctttatt
      661 gctttccttt tcggtagtgc ctgatttttc ggtattcgac tttacttctt ctgcaagaac
      721 attattttct ttaacggaag atgaagtttt tgaagtggaa gtcgtttcca cattcattcc
      781 attaacaacc ttgctattat ctgaagtttc tttgttatca atagcatgat caataataga
      841 attctctgaa ataatattct tctcaagtcg accgatctct gttttgtttt cgtctgctga
      901 ttctgtattc aaattataaa taagaatact tcctccaatg acacccacca gtgaagcagc
      961 gattacccac gggcgatatg aaataccttt cttggggaga gagcgctcaa gtcgtgtcca
     1021 ttgcgttgcg tcaaatggcg actcatgatt ttcaaccttt ttacgaagca tggattcgaa
     1081 tgcatcaccg gccgaagcat cgagcttttg ctccatggcc gaccaggctg catcatcaaa
     1141 aggcatctcg aaattttcga gaccatcctt tatctgtttt tcaaatccgt tcaaacggtt
     1201 cattctttta tacttaattg cttttgaagc aaaattttca atctcatttt ggccttggca
     1261 aaatttgact ttgacgtacc aacactaatc tgaagagcgt cggcaatgtc tttgtgcgtg
     1321 tagttctcga aaacatacaa attgaatact gcctgatagg ccggactcaa cttctgcatg
     1381 gcttccatga tcatttcagg cgagacattg gcatatggtg ccaaatcttc gttttcctcc
     1441 tggaatgctc ctaccgaatc tttgtcctca tctttgatga gagagtcatc atcgattgta
     1501 agcatttgct tattacgtct gatatggtca attgcagtgt taaccacaat tcttctcagc
     1561 caaccttcga acgaaccgga aaaattaaaa tccccgatct tatcgaatgt ctttataaaa
     1621 gcatcctgaa gcatatcctg tgcagtatcc ttgtcacggg tataccgcat gcacacactc
     1681 atcatttttc caaagaactc tttaaagacc tgctcctgac accgccggtc gccgtccata
     1741 catccgcgta tggcaacttc cagttgctca ggggttagtt tcatgggtct tgtgctcata
     1801 tgacgagggg ttgtcaaaag ggttgcctat tctggagaaa aaccatttca cccccttaaa
     1861 agttacaggt aatcaggcaa ttccacacaa cctttggact tacatacttc aaagataagc
     1921 gattagttcg gggaaggggg aaagtgttga aatcttgaaa actcttggag ccatcttgaa
     1981 gtcaacataa aaggaaggac aaattcttct aatttagcgc tccgaacgaa ttacaaaccc
     2041 aaatcctcac caccatgaaa attacagtag ttggagccgg agctgttgga gctacctgtg
     2101 ccgataacat tatccgtaaa gaacttgcag aagaagttgt attgctggat attaaagaag
     2161 gtcttg
//
LOCUS       DAOMYG010000503         2141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_19919_length_2141_cov_11.146213, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000503 DAOMYG010000000
VERSION     DAOMYG010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19919_length_2141_cov_11.146213"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1581)
                     /locus_tag="PK610_13060"
     CDS             complement(<1..1581)
                     /locus_tag="PK610_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36917.1"
                     /translation="MSAFAQTGPGGVGNSTSNNMWYSGDGGAFTNAAGTTQATVGQQI
                     YVWRDRSGNTRNATQATAANRPLFHRQTAAANGQPGLRFTGDLFIDGPSPGIANNSSY
                     TYLMVFRDTSTVTGATNDGAGTFMLDRTTATNELVSLKPVTGNFYFYQKRNDAGGGLG
                     GVTGSTTINTTIKAITMRRNYGTNYQLFHNNQQQGGNVADADGATTPPAPRIGRHATT
                     SGGGIRGYIYEFIVYNFALNSAQMIIVNNYLAAKYNFALAVDDLYTMDDPANGNYDYE
                     VAGIGRVDNSNTHTDARGSSIVRMTNPSALGNNRFLFWGHDNGTMTTNNTVDVDGIVI
                     QSRLNRIWRAQKVANIGTADVSFDVTGFTSVNGAHLRLLIDRDGDGFFDNDVTPLTGT
                     FAANTITFTGVNFQNGDRFTLGSINVSQTPLPVELLDLNALLLNDHEALITWKTATET
                     NSSFFEIQRSSDGSDWTAIGQVIAAGFSSVYRNYLFKDYAPLSGTSYYRLNMVDQDGL
                     NELSVIRVLENHRGSAVIA"
     gene            complement(1673..>2141)
                     /locus_tag="PK610_13065"
     CDS             complement(1673..>2141)
                     /locus_tag="PK610_13065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ36918.1"
                     /translation="RSIEFHFVVQRSVDGIQWIGIGQIMAAGFSVLHRNYNFTDHAPL
                     SCTSYYRLRMVDLDGSNDFSSVRVVDAASENSHWINVFPNPVSDWITLKSIDDKPISG
                     IEIYDVAGRKVREVAHSVGASLTIDVSDLPPGTYYFVYNQGSRPGVSPFVRYQ"
BASE COUNT          670 a          471 c          407 g          593 t
ORIGIN      
        1 agcaataaca gcagatccac gatggttttc caaaacccga ataacagaca actcattaag
       61 cccatcctga tctaccatgt tcaaccgata atacgatgta cctgaaagtg gtgcataatc
      121 tttaaataaa taattcctgt aaaccgaaga gaaaccggca gctataacct gcccgattgc
      181 tgtccaatca gagccatcgg atgatcgctg aatttcgaaa aatgagctat tggtctcagt
      241 tgctgttttc caggtaatca aagcttcatg atcattcaaa agcaatgcat taagatcaag
      301 caattcaaca ggaagaggag tttgactgac attaattgag cctaaggtaa agcgatcgcc
      361 gttttgaaag ttaacgccgg taaaagtaat ggtgttagct gcaaaagttc cggttaacgg
      421 agtgacatca ttatcaaaga aaccatcacc gtctctatcg attagcaaac gcaagtgagc
      481 gccgttgacg cttgtaaatc cggtaacatc aaatgaaaca tctgctgttc caatattagc
      541 aactttttgt gctcgccaaa ttcgatttaa tctgctttga attacaatgc catcaacatc
      601 aaccgtattg ttcgttgtca ttgtaccatt atcatgtccc caaaaaagga atcgattatt
      661 tcctaatgcg cttggatttg tcattctaac tatcgaggaa cctctcgcat cagtatgtgt
      721 attggaatta tccacacgcc caattcccgc tacttcgtaa tcataattac catttgcagg
      781 atcatccatt gtataaaggt catctacagc taatgcaaaa ttgtatttag ccgccaaata
      841 attattgaca ataatcattt gcgcagaatt gagcgcaaaa ttgtaaacaa taaactcata
      901 aatataacca cgaataccgc ctccggaagt tgttgcatgg cgaccaattc gtggtgcagg
      961 aggagtggtt gctccgtctg catcagccac attacctcct tgttgttgat tattatgaaa
     1021 caactgataa tttgttccat aatttcttcg catggtaata gccttaattg ttgtgtttat
     1081 ggttgttgaa ccagtaacac cccctaatcc acctcctgca tcgtttctct tttgatagaa
     1141 ataaaaattt cctgttacag gttttaatga aaccaattca tttgttgcag ttgtacgatc
     1201 caacataaat gttccggctc catcattagt ggcaccagtc actgtagaag tatcgcgaaa
     1261 gaccatcaaa taggtataac tactattatt tgcgattcct ggtgaaggac catctataaa
     1321 aagatctccg gtaaatcgaa gtccgggctg accattggct gctgctgtct gacgatgaaa
     1381 caaaggtctg tttgctgcag tggcctgcgt tgcatttcgt gtattaccgg aacgatcgcg
     1441 ccaaacgtat atctgctgac ctacagtagc ctgtgtagta cctgctgcat tggtaaaagc
     1501 accaccgtcg ccggaatacc acatgttgtt actggttgaa ttaccaaccc cacccgggcc
     1561 ggtttgtgca aatgctgaca aattacaaag ggaaaaaccg agcagcagta ttgacctaat
     1621 tttcattcta taaatctatt gaaatctaac gaaaatagga aattcggatg agctattggt
     1681 accttacgaa tggagacacg cctggcctag acccttgatt ataaacgaag taatacgtcc
     1741 cgggcggcag atcagaaaca tcaattgtta acgatgcccc tacagaatga gcaacttccc
     1801 gtacttttcg accagccaca tcataaattt caattcctga aatgggtttg tcgtctatag
     1861 atttaagggt aatccaatca ctgacgggat tcggaaaaac attaatccaa tgcgaatttt
     1921 ctgaagctgc atcaacaacc ctaaccgaag aaaaatcatt gcttccatca agatcaacca
     1981 tccgcaaacg gtaataagaa gtacacgata atggagcatg atcggtgaaa ttgtaattac
     2041 gatgtaaaac agaaaatcct gcggccatga tttgaccgat tccgatccat tgaattccat
     2101 ccactgaacg ttgaacaaca aaatggaatt caatggatcg g
//
LOCUS       DAOMYG010000504         2128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_20091_length_2128_cov_6.194404, whole genome shotgun sequence.
ACCESSION   DAOMYG010000504 DAOMYG010000000
VERSION     DAOMYG010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20091_length_2128_cov_6.194404"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PK610_13070"
     CDS             <1..554
                     /locus_tag="PK610_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36919.1"
                     /translation="VFGIANLFGQPGKFIPPLHIDNFFLTALAAYFFFFSEKPIPKMA
                     LLSLCCSLAISTWLDLNDISSSTVIFGMLSLVFFGFSLIAHSLDYLQNFKSAEQSTNK
                     IWLVFLMLFFATFFFLIAARFYTENRLMSYSYWASGFMAILLLIKNRNSSPIQLGTYG
                     YWVIIALSALFDLGNYLAIRALV"
     gene            complement(551..1666)
                     /locus_tag="PK610_13075"
     CDS             complement(551..1666)
                     /locus_tag="PK610_13075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRJ36920.1"
                     /translation="MQLSYWEKERFFEQVDACVIGSGIVGLSTAIELKRQRPDWKVII
                     LERGFLPYGASTRNAGFACFGSVSELMDDFGRMGESQTIALVERRWRGLLNLRQLLGD
                     QSIGLENFGGYELFDDAERYELAADTIEKFNALLEAIIGKQVYATNPSAVDHFGFNGF
                     IGTIKNQFESQINTGEMMHALLKLARKEGVIILNGIGVDQVEGKCIVTTAGFSFEANA
                     VIVTTNGFAKQLLPQLDVEPARAQVLVTEPIPGLKFKGTFHFDEGYYYFRNIGTRILL
                     GGGRNLDFAGENSTNMIQTPIIQDRLEQMLFERIVPYAKPKIEMRWSGVMGVGETRGP
                     LVQKLNEHLYCAVRMGGMGVAIGSLVGKEVAALVVEN"
     gene            1779..>2128
                     /locus_tag="PK610_13080"
     CDS             1779..>2128
                     /locus_tag="PK610_13080"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRJ36921.1"
                     /translation="MRNRLFLVFFLLLGTALHLHAQEKPLAYFENPRVTDKLTGKPMA
                     NVTVTVESSGSTVETKTTGSNGKFKLNPIKLDQKYKITFSAPGYQTRFIEVDLTGIPP
                     AHIPEEGYNFPLDMS"
BASE COUNT          575 a          532 c          364 g          657 t
ORIGIN      
        1 gtgtgtttgg aatagccaat ttgtttggac aacccgggaa atttattcct cctcttcaca
       61 ttgataattt ttttctgaca gcccttgcag cgtatttttt ctttttttca gaaaaaccca
      121 tcccaaaaat ggccctgcta agcctctgtt gttcccttgc aatcagcaca tggctcgact
      181 tgaatgatat ctcatcttca acagtaattt tcggtatgct ctcacttgta tttttcggtt
      241 tttctctaat cgcacatagc ctggattacc tgcaaaattt caaatcggcc gaacaaagca
      301 ccaacaaaat atggctggtt tttttaatgt tgttttttgc tacttttttc tttttgattg
      361 cagcacgttt ttatactgag aatcgcttaa tgagttactc ctattgggca agcggtttta
      421 tggccatcct tctcctgatc aaaaacagaa actcatcccc cattcaattg ggcacatacg
      481 gttattgggt gatcatagca ttatctgcat tatttgatct tggaaattat cttgcaattc
      541 gtgcattggt ttaattttcc actaccaaag ctgccacttc ttttcccacc aatgaaccaa
      601 tagccacacc cattcccccc atgcgtacgg cacaatacaa atgttcattt agcttttgca
      661 ccaacgggcc cctggtttct cccactccca ttacaccgct ccagcgcatt tctatttttg
      721 gttttgcata gggaactata cgctcgaaca gcatttgctc caatcgatcc tgaatgattg
      781 gagtctggat catattagtg ctgttttcac ccgcaaaatc taaatttctt ccaccgccaa
      841 gcaggatgcg ggtgcctata tttctgaaat agtaataacc ctcatcaaag tggaaagtcc
      901 ctttaaattt taatccggga ataggttctg taaccaaaac ctgagctctt gcgggttcaa
      961 catccaattg aggcaatagt tgttttgcaa acccatttgt ggtaacaata accgcgttgg
     1021 cttcaaatga aaaacccgca gtggtaacaa tgcatttgcc ttctacctga tctacgccaa
     1081 ttccgttcag aatgataaca ccttctttcc ttgccaattt tagcaatgca tgcatcattt
     1141 cccccgtatt tatctgggat tcaaattgat tttttatcgt cccgataaat ccattaaagc
     1201 caaaatgatc cactgccgag ggattggtag cgtaaacctg ctttccaata attgcttcca
     1261 gtagagcatt aaacttttca attgtatcgg ctgccaactc ataacgctcc gcatcatcaa
     1321 acaattcata gccaccgaag ttttccaatc cgatcgactg atcgccgagt aactgcctaa
     1381 gattcaacaa tccccgccat cttcgttcca ctaatgcaat tgtttgggac tcccccattc
     1441 ttccgaagtc atccatcaat tcgctaacac ttccaaaaca agcaaagcct gcattgcgcg
     1501 tacttgcccc gtagggaagg aaacctcttt ccaatataat caccttccag tcaggacgtt
     1561 gcctttttaa ttcaatagcc gttgaaaggc ctacaattcc gctgccgatc acacaggcat
     1621 cgacctgttc aaaaaaacgc tccttttccc agtaactcaa ttgcataggg tccgttatat
     1681 ctctatgttt tccacgaatc taaagcaatt ttcacgatat ttgtaagata gccttcaacc
     1741 actttaaggc ttgagtgtac cgaatgataa acgcgttgat gaggaaccgg ctttttcttg
     1801 ttttcttttt gttgcttgga acggcattgc atctccatgc acaggagaaa ccccttgcct
     1861 atttcgaaaa tccgagggta acagacaagc ttaccggcaa accaatggcc aacgtaactg
     1921 ttacggttga atcgagcggc agcacggttg aaaccaaaac caccggatcg aatggtaaat
     1981 tcaaattaaa cccgatcaag cttgatcaga aatacaaaat aacattttcc gcgcccggtt
     2041 atcaaacccg atttattgaa gttgatttaa ctggaattcc ccctgcgcat attcctgaag
     2101 aaggctataa tttccctttg gatatgtc
//
LOCUS       DAOMYG010000505         2121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_20184_length_2121_cov_7.876089, whole genome shotgun sequence.
ACCESSION   DAOMYG010000505 DAOMYG010000000
VERSION     DAOMYG010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20184_length_2121_cov_7.876089"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PK610_13085"
     CDS             <1..481
                     /locus_tag="PK610_13085"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HRJ36922.1"
                     /translation="KEKVEQVGMKDKVDKFVETMNRGAEEACKEAAPIFVNAITGMSI
                     GDGFGILNGPDNAATEYLKEKTSSQLYTTFKPKVQTALQTVKLTEYWNPVITRYNQII
                     KISGGDQVNPDLDDYVTKGAMGGLFLLIADEEKKIRKDPMARVSDILKSVFGSLDNK"
     gene            484..1377
                     /locus_tag="PK610_13090"
     CDS             484..1377
                     /locus_tag="PK610_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018943657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HRJ36923.1"
                     /translation="MQIAAKRQSDEPIPESELIVTPAGAIYHLNLRPDQLAEKIILVG
                     DQNRVARISRHFDTIEHKVENREFITHTGTYKGKRVSAISTGIGTDNIDIVLNELDAL
                     VNIDLNTRMIKPQHSALQMVRLGTSGSLQEDIQVDTAVVSSYAVGFDGVLGFYRNQPE
                     EDEFALEQAFIDHMMWSPKLNPPYFVRANEELFQRIGKGMTVGITATSNGFYGPQGRI
                     LRLPVLDSDINEKLRSFSGNGHRICNYEMETSALYGLAALLGHQATTVCAIIANRYRK
                     AYSKDYHKVIDELVELVLDRI"
     gene            1597..>2121
                     /locus_tag="PK610_13095"
     CDS             1597..>2121
                     /locus_tag="PK610_13095"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRJ36924.1"
                     /translation="MMTSMSDDKEINALITLIDDPDERVYQHIRKKLMDFGYGVIPAL
                     ENYWEHNKFGLSFQSRIEDLIHDIQFDHVKTGLSHWVRDGANDLLRGVLLVNRYQYPD
                     LDEGKIRKKLYQLKQDVWLELNDGLTAFEQIRVINHILFDVHNLSGNKKNYHAPQNSY
                     LNNVLETGKGNPLSL"
BASE COUNT          649 a          440 c          483 g          549 t
ORIGIN      
        1 gaaggagaaa gttgaacagg tgggaatgaa ggataaagtc gacaagtttg tagaaaccat
       61 gaaccgtggt gctgaagaag cttgtaaaga agcggctcca atttttgtaa atgcaattac
      121 cggcatgagc ataggagatg gattcggtat tttaaatggt ccggacaatg cggcaacgga
      181 atacctgaag gaaaaaactt cttcacaact ctacacaacg tttaaaccca aggttcagac
      241 tgccctgcaa acggtaaaac tgaccgaata ctggaatccg gttattaccc gttacaacca
      301 gatcatcaaa atcagcggtg gagatcaggt aaatcccgat ctcgatgact acgttaccaa
      361 aggcgcaatg ggcggactgt ttttactgat tgccgatgag gaaaaaaaga ttcgcaaaga
      421 ccccatggca cgtgtttccg atatcctgaa atcggttttc ggaagtctgg ataataaata
      481 accatgcaaa tcgctgcaaa acggcagagt gatgaaccta ttccggaatc ggaactgatt
      541 gtcactcctg cgggtgctat ttaccacctg aatctgcgtc ccgatcaact cgctgaaaag
      601 ataattcttg ttggcgacca aaatcgtgtt gcacgcattt cgaggcactt tgataccatt
      661 gaacataaag ttgaaaacag agagttcatc acccataccg gaacgtacaa gggaaaacgc
      721 gtctctgcaa tcagcaccgg aataggcaca gacaatattg acatagttct gaatgaactt
      781 gatgcattgg tcaatattga tctgaacacc cgcatgatta aaccacagca ttcagcactg
      841 cagatggtac gactgggaac ttcaggatcc ttgcaggaag atattcaggt ggacactgct
      901 gttgttagct cctatgcggt aggtttcgac ggtgtgttag gattttaccg caaccagccg
      961 gaagaagatg agtttgcgtt ggaacaagcc tttatcgatc acatgatgtg gtcgcccaaa
     1021 ttaaatccac cttattttgt gcgtgcaaac gaagagttgt ttcaacgcat cggaaaaggt
     1081 atgaccgtag gaataacagc tacctcaaac ggattttacg gaccgcaggg acgaattctg
     1141 cgtttaccgg ttctcgatag cgatatcaat gaaaaattaa gaagcttttc gggaaatggt
     1201 caccgcattt gtaattacga aatggaaacc tctgcgcttt acggtttggc agcattgttg
     1261 ggacaccagg ccacaaccgt atgtgcaatt attgccaatc gctaccgcaa agcctattct
     1321 aaagattatc ataaagtaat tgatgaattg gtggaacttg ttcttgacag gatttagggg
     1381 agattagtta ttagttattt gtgagcggaa agtggtgaag tgtgatttcc gatacttatg
     1441 gttgaaattg gtaggggaat agcacctttt gattcagttt taacagatac cggtcacctt
     1501 tcacgtgtca ccaaaaatat attactcaac actcccctat tcataattac ctctgccacc
     1561 ccgaaacctt gagggcttct cgtcgttaat attgcaatga tgacgagtat gtccgacgac
     1621 aaagagatca atgcactgat caccctgatt gatgatccgg acgagcgcgt gtaccagcat
     1681 atccgaaaga agctgatgga tttcggttat ggggtaatac cggcacttga aaattactgg
     1741 gaacacaata agttcggtct ctcctttcag agcagaattg aagatctgat tcacgatatt
     1801 cagtttgacc atgtaaaaac cggtctatct cattgggtac gcgatggtgc aaatgatctt
     1861 ttgagaggcg ttttacttgt aaaccgctac caatatccgg atctcgatga aggaaagatc
     1921 agaaagaaat tataccagct aaagcaggat gtttggctcg agctgaatga cggattaacg
     1981 gcctttgagc aaatcagggt gattaaccat attctttttg atgttcacaa cctatcgggc
     2041 aacaaaaaaa actaccacgc accacaaaat tcttatctca acaatgtcct tgaaaccggc
     2101 aaaggaaatc cattgtcgct t
//
LOCUS       DAOMYG010000506         2119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_20217_length_2119_cov_8.822674, whole genome shotgun sequence.
ACCESSION   DAOMYG010000506 DAOMYG010000000
VERSION     DAOMYG010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20217_length_2119_cov_8.822674"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PK610_13100"
     CDS             <1..561
                     /locus_tag="PK610_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36925.1"
                     /translation="IQAFFHLLNSFLFICIFSIGLLTFPVIMVAEKFPEYGNLYSIFS
                     VFYLSMIFISIYYFASEIHHAKNKVKGFFAFIVLYPVFLCVSMGLSLYNTIGVLEGYT
                     GRKSAFVRTPKFNITGKKGTWSKSKYAIQKLRPVVLLEGFFFLLFGIACFEVAKTGFS
                     LSLVFYGMLTIGYGFVFLGSIIHSKT"
     gene            637..2025
                     /locus_tag="PK610_13105"
     CDS             637..2025
                     /locus_tag="PK610_13105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRJ36926.1"
                     /translation="MLLGFVILYMVFTVAMGFFASRFISSSTDFVQAGRRLPAFFNAT
                     ALFALWFGSETVFGASSRFMEEGLIGVVEDPFGGVLCLLLFAIFFVRKLYRLNMLTIG
                     DLFERAYGKRVEVVSSVFMLITFFGYIAAQLIALAIILKSVTGLETEYGIVLCAVIVT
                     GYTFVGGMWAVSITDFVQSIFIVIGMIIIAVYMANAAGGVDVIFNSAPEGTFTFLPER
                     DPLGWSNWIALWLTLGLGSLASQDIFQRVNSARSEKAAVRSTYIGALMYLIISMFPLF
                     IALSAKVLYPNENFSDTQEVIPRLVLAHAPMVVQVLFFGSLLSAVLSTCSGALLAPAS
                     ILSENLIKPLSKREYSDQQFLWLVRISIVIMAIISTVIALGGSDIFELVSVSSVLGIV
                     SLLVPMVAALYWKKANAAGAMLSMFSGMGTWLILEYMLHPKLEPFLIALPFSILGLVL
                     GTYLFPVKIAQD"
BASE COUNT          482 a          369 c          481 g          787 t
ORIGIN      
        1 atccaggcat tttttcattt gttgaacagc tttttgttca tctgtatttt tagtatcgga
       61 ttactaacct ttcctgttat aatggttgcc gaaaaattcc cggaatacgg caatttatat
      121 agcattttta gtgtgtttta cctttcaatg atttttattt cgatctacta ttttgcttcc
      181 gaaattcatc atgcaaaaaa caaagtcaaa ggattttttg cgtttattgt tttgtatccg
      241 gtgtttttat gtgtttcgat gggacttagt ctttacaata ctattggtgt tctcgagggg
      301 tatacaggtc gaaaaagtgc ttttgtgcgt acaccgaaat ttaatattac cggtaaaaaa
      361 ggtacttggt caaaaagtaa gtatgccatc cagaaattac gtcccgtagt gctgcttgaa
      421 ggtttctttt ttcttctgtt tggaatcgcc tgttttgaag tggcgaagac cggtttttcc
      481 ttaagtctgg tgttttatgg aatgcttacc atcgggtacg gatttgtttt tctgggttct
      541 atcattcatt cgaaaacata aaactgtatt ttggtcgtga attttacttt caatattatc
      601 atttcggtta atgcttgtag tttaaattag ttcgcaatgt tacttggctt tgtcattcta
      661 tatatggttt ttacggtggc aatgggtttt tttgcctctc gttttataag cagcagtacc
      721 gattttgttc aggcgggcag acgattacct gcctttttta atgcaacagc attgttcgct
      781 ttgtggtttg gttctgaaac tgtatttggt gcatcatccc ggtttatgga agaaggattg
      841 atcggtgtag tggaagatcc atttggcggt gtattgtgtt tattgctgtt cgctatattt
      901 tttgttcgca agttgtaccg gttaaatatg cttaccatcg gcgacctttt cgaacgcgcg
      961 tatgggaagc gggtggaagt ggtttcttcg gtttttatgc tgattacttt tttcggatat
     1021 atcgcagcac aactaatcgc ccttgcgata attctaaaat cggtaaccgg actcgaaaca
     1081 gaatacggaa tcgttctttg tgctgtaatt gttaccggct ataccttcgt aggtggaatg
     1141 tgggcggtat ccattaccga ttttgtgcaa agtattttta ttgtgattgg aatgatcatt
     1201 attgcggtgt acatggctaa tgctgcaggt ggagtagatg tgatttttaa cagtgctccg
     1261 gaagggacat tcaccttttt acccgaacgc gaccctttgg gttggtcgaa ctggattgca
     1321 ttatggctta cactcggatt aggatcgttg gcatcgcagg atatttttca gcgggttaat
     1381 tcagcgcgtt ctgaaaaagc tgctgtgcgt tcaacttata ttggcgcatt gatgtatttg
     1441 atcatttcta tgtttccgct ttttattgcg ctatcagcaa aagtgcttta ccccaatgag
     1501 aatttttcag atacgcagga agtcataccg cgactggtac ttgctcatgc gccaatggtt
     1561 gtgcaggtat tgttttttgg ttcattattg agtgcggttc tctccacttg cagcggtgca
     1621 ttgctggctc ctgccagtat cctttccgaa aacctgatca aaccgctttc aaaaagagaa
     1681 tattcggatc aacagttttt atggctggta aggatcagta ttgtgatcat ggcaataatt
     1741 tctacggtaa ttgccttggg aggatcagat atttttgaat tggtaagtgt gagttcggtt
     1801 ttgggaattg tatcgctgct tgtcccgatg gttgccgcat tgtattggaa aaaggccaat
     1861 gctgcaggag caatgctgtc gatgttttcc ggtatgggaa cctggttgat tttggagtat
     1921 atgttacatc ctaaactcga accattcctg atcgcacttc cgttttcaat tcttggatta
     1981 gtgttgggga cttatttatt tccagttaaa attgctcagg attaaatatt ccttttcgtt
     2041 ttatttggaa aggacttcaa atgtctgcgt gatttttgcc ccttcttcat caatgatggt
     2101 aagcgtgtgc attcctctg
//
LOCUS       DAOMYG010000507         2118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_20233_length_2118_cov_8.070771, whole genome shotgun sequence.
ACCESSION   DAOMYG010000507 DAOMYG010000000
VERSION     DAOMYG010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20233_length_2118_cov_8.070771"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2118
                     /locus_tag="PK610_13110"
     CDS             <1..>2118
                     /locus_tag="PK610_13110"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRJ36927.1"
                     /translation="SPMIRAAGIFLLLFQLSFVLSAQRTDYRFDHLDLRKGAGVNRLN
                     ILGFDSIGRAWFITERGIASYDGFLIRNTGEPKGGENALLDRTISGAFLGEDGKIYVG
                     YFEAGLSIYDPIADKWKHEVPDTLHPNGFPNGMVSRIKVEKDGTVFLLTWGAGFSIKR
                     PGQDWKNYFPVVDDPTSLSSTRLKEVVRDIDGTYLIGSWEGDGYENRLQQLNIETGKF
                     SDFPIGDYLNGIPEKEQLYIRSSLRIIHSIYIDRTGNLWVATYSGLIFIDRKNKSAKR
                     ITARLYDNQGNLENTRTIEEGPNGKLWVGTQSTGILVVDPRLGTCVNLHHDVNVHQGI
                     SDDRIRNIKRDKNGNLWISTGSGLIDVLVPVKQEFEMISNQILKADFNDRSAEIMPLT
                     RVFSDSRGMIYAMSASGISLYNRKESRLDRMINLSEIQYKGKSVHPDLIKHTKVANCM
                     AELPNGDILIGSRSALFRFDPITNTIKKNWGLARFSVDCFAWSPDSTYIWFFGYHSRE
                     LMKYHIREDEIEIVDVFDPNRWINYRQFFDLGNGRLLFEAGERDGFYLYSVAEKSFER
                     WSKNEKTHFFPDSTILFLNQDAKRNVWIGTETGLYLFDRKLNKVEPYHELLGITNKPV
                     HVIVSDRSGVMWIGIEGDLLRYDPISGEKFLFTRGFGCEAGLFGSLTPFVDNNGSIFF
                     TTLKGIMIIDPDNIYPDGTIPVLS"
BASE COUNT          606 a          358 c          506 g          648 t
ORIGIN      
        1 aagtcccatg atcagagctg ccggaatttt tcttcttctt tttcaattgt cgtttgtgct
       61 ttcggcgcag cgaacagatt atcgttttga tcatcttgat ttgcgaaagg gagccggcgt
      121 aaatcgatta aatattcttg gattcgattc catcgggcgg gcatggttta ttactgaaag
      181 aggaattgcc agttacgatg gttttttaat tcgcaatacc ggtgaaccaa aaggcggtga
      241 aaatgccttg ttggacagaa cgatctcagg tgcatttctt ggtgaagacg gaaaaattta
      301 tgtaggctat tttgaagcag gattgtcgat ttatgatccg atcgcagata aatggaaaca
      361 tgaagtgccg gataccttgc atcccaatgg ttttcccaat ggaatggtat ctcgtattaa
      421 ggtagaaaaa gacgggactg ttttcctttt aacctgggga gcaggttttt ccattaagcg
      481 gccgggacag gattggaaga attatttccc tgttgtggat gatccgacat cattaagcag
      541 cacccgattg aaagaggtgg ttcgtgatat tgacggaact tacttaattg gtagttggga
      601 aggtgatggt tacgaaaatc gcttgcaaca gctaaatatt gaaaccggta aattttctga
      661 ttttcctatt ggagattatt tgaatggcat tccagaaaag gaacagcttt atatccgctc
      721 atcgctccgt atcattcata gtatttacat tgatagaact gggaatttat gggttgcaac
      781 ctatagtgga ctgattttta tcgatcgaaa aaataaaagt gcaaaacgga ttactgcaag
      841 attgtacgat aatcagggta acctcgaaaa cacccggaca attgaagaag gtccgaatgg
      901 taaattatgg gtgggaaccc agtcgaccgg aatattagtg gttgatcctc gcttaggaac
      961 gtgtgttaac ctacatcacg atgtaaatgt tcaccaagga atttcagatg atcgtattcg
     1021 taacatcaaa cgcgataaaa acggaaacct atggataagt acaggctccg gactgattga
     1081 tgttctcgtt ccggtgaaac aggagtttga aatgatttca aaccaaattc tcaaagctga
     1141 ttttaatgat cgctcagcgg agattatgcc cttaacgcgt gttttttcag attctcgagg
     1201 catgatttac gctatgagcg cgagtggaat tagcttgtac aaccgaaaag aatcgcgatt
     1261 ggatcgtatg atcaatttgt cggaaattca gtacaaggga aaaagtgttc atccggattt
     1321 gatcaagcat acaaaagtcg ccaattgtat ggctgaatta ccaaacggcg acattttaat
     1381 tggttcgagg tcggctttgt ttcgatttga tccgattacg aatacaataa aaaagaattg
     1441 gggacttgct cggttttctg ttgattgttt tgcctggtct cctgatagta catacatctg
     1501 gttttttgga taccacagca gagagttgat gaagtatcat attcgcgaag atgaaattga
     1561 aattgtcgat gtcttcgatc ccaaccgttg gatcaattac aggcagtttt ttgatcttgg
     1621 caatggtcgt ctgctttttg aggcaggtga acgcgatgga ttctatttgt attccgtagc
     1681 agaaaagtca tttgagcgct ggagtaaaaa cgaaaaaaca catttttttc ccgactcaac
     1741 catacttttt ttgaatcagg atgccaaacg caatgtgtgg attgggactg aaacaggact
     1801 atatttgttc gatagaaaat tgaataaagt tgagccgtat catgaacttc ttggaattac
     1861 gaataagcct gttcatgtaa ttgtcagcga tcgaagtggg gtgatgtgga tagggataga
     1921 gggagattta ctgcgttatg atcctattag cggggagaag tttttattta ccagaggttt
     1981 tggttgtgag gccggattgt ttggatcgtt aaccccgttt gttgataata acggaagtat
     2041 ctttttcacc acgctgaaag ggatcatgat aatcgatcct gataacattt atccggatgg
     2101 tacaattccg gttttatc
//
LOCUS       DAOMYG010000508         2105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_20426_length_2105_cov_5.737073, whole genome shotgun sequence.
ACCESSION   DAOMYG010000508 DAOMYG010000000
VERSION     DAOMYG010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20426_length_2105_cov_5.737073"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PK610_13115"
     CDS             <1..230
                     /locus_tag="PK610_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36928.1"
                     /translation="VQTSQGGVAPCRGVVTNESEIKAFSEKLHLISEEMDAFLRELNE
                     MYLKKKEENELKLEIDNGIDNRSSLFNRFQE"
     gene            322..750
                     /gene="arr"
                     /locus_tag="PK610_13120"
     CDS             322..750
                     /gene="arr"
                     /locus_tag="PK610_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HRJ36929.1"
                     /translation="MTENKVSGAIPFAQTYFHGTKADLRLGDLIVPGYNSNFGQRKNA
                     KYIFLTATLDAAIWGAELAEGTGRERIYLVEPMGAIEDDPDLTDKKFPGNPTMSYRST
                     APFKVVGELAHWQSHPEDQVKAMKDALERLKEQGINSLNE"
     gene            1058..1324
                     /locus_tag="PK610_13125"
     CDS             1058..1324
                     /locus_tag="PK610_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36930.1"
                     /translation="MKSKRLFLILSVVAVLLAIPAIAMQFTDEVKWDAADFLLMGLLL
                     LSTGILIEVLFRSVKKRNYRIIGSGIVLGVFLLVWAELAVGIFD"
     gene            1447..>2105
                     /locus_tag="PK610_13130"
     CDS             1447..>2105
                     /locus_tag="PK610_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019671533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRJ36931.1"
                     /translation="MNNPILRIRPLGFQWATLDPFIFCVHHNDRFPKGNAQQGPAVSL
                     NGRQKGSDFSGKDGWSMYHGDAIPGFPGHPHKGFETITVVRKGLVDHTDSMGASGRYG
                     DGDVQWMTAGKGVQHAEMFPLVHEEKENPMELFQIWLNLPAKHKHADPHYIMFWKEKL
                     PHAEYVSPNGAKVKAEIIAGDLWGVRALNPPPKSWAAESVNAVAIWIIHCPAGASFKL
                     PA"
BASE COUNT          576 a          371 c          495 g          663 t
ORIGIN      
        1 gagtccagac gtcccaaggg ggagtagctc cctgccgcgg cgtcgtcacg aatgaatcgg
       61 agattaaggc tttttcagaa aagctgcacc ttatttccga agaaatggat gctttcctca
      121 gggaattaaa tgagatgtac ttaaagaaga aggaagagaa tgaattgaaa ctggaaattg
      181 ataatggaat tgataaccgg agttcattgt ttaatcgctt tcaggaatag ggttaaattt
      241 attgctatta tcaacttatt gaatcaacgt tttttatgaa gtaagcaatt tgacattttt
      301 tttaaaagta aaaactgagt catgacagaa aataaagttt ccggagccat tccttttgcg
      361 caaacgtatt ttcatggcac caaggctgat cttcggttgg gcgatctgat tgttccggga
      421 tacaattcta attttggtca gcgaaagaat gcgaaataca tttttctgac ggccacactt
      481 gatgcggcta tttggggtgc tgaacttgca gaaggaaccg gaagagaaag aatttacctt
      541 gttgagccta tgggtgcaat tgaagatgat ccggatctga cagataaaaa gtttccggga
      601 aatcctacaa tgtcttatcg ttctacagcg ccttttaaag ttgtggggga gcttgcacat
      661 tggcagtcgc accctgagga tcaggtgaaa gcaatgaaag atgctctgga acgtttaaag
      721 gagcagggaa taaattcgct gaatgaatga tttcacagga atttgatggc ctgaagttat
      781 ttaataagat attcaggttc agaacaattg ttgagttttc tttggtgcta aggtatccgg
      841 tgacttttcg tttccgtgac tgttttcctt ttattaagaa gtgtatgcta tttagagagt
      901 tgccttttcc ggctcaaaat gttaaaatat cctattaaaa atcaagcagc tttttcattt
      961 gttatttttg ttgcagacgt tctatcggta gttatttatg gccttccgtt atgaacatta
     1021 tcgaaaacga actattcatt tatccagatc atttactatg aaaagcaaaa gactttttct
     1081 tattctttct gttgtagcag tattgttggc aatacccgca attgcaatgc agtttactga
     1141 tgaggtgaaa tgggatgctg cagactttct gctaatggga ctgttattgt tatcgacagg
     1201 aattctgatt gaggtcttat tccgtagtgt taaaaagcgg aattaccgaa ttatcggatc
     1261 tggaattgtg ctgggtgtat tccttttggt ttgggctgaa ctggcggtcg gaatttttga
     1321 ttaggagatg atttggtggt gaagtgaagt ttttttctgg tttgattgat cggaacatta
     1381 gattattaat ttcccttgat attaggtttt tcccgaactt tagccttcca caattgtttc
     1441 tcagttatga ataatccaat cctccgtatt cgtccgcttg gctttcaatg ggctacgctc
     1501 gatcctttta ttttttgtgt acatcacaac gatcgttttc caaaggggaa tgcgcaacaa
     1561 ggtcctgctg tttctttaaa tggtcgccag aagggaagtg atttttccgg taaggatgga
     1621 tggagtatgt atcacggcga tgctattccc ggttttccgg gtcatccgca taaaggattc
     1681 gaaacgataa ccgttgtgcg aaaaggattg gtggatcaca ctgattctat gggtgcatca
     1741 ggaaggtatg gtgatggcga tgtacaatgg atgacggcag gaaaaggtgt acagcatgct
     1801 gagatgtttc ccttagtgca tgaggagaag gaaaacccga tggaattgtt tcagatctgg
     1861 ctcaatctcc ctgccaaaca caaacatgcc gatccgcatt acatcatgtt ctggaaagaa
     1921 aaacttcctc atgctgaata tgtttctccc aatggagcaa aagtaaaagc agaaatcatt
     1981 gcaggcgatc tttggggcgt gagagcactt aatcctcctc cgaaatcctg ggcagcagaa
     2041 tcggtgaacg ctgtagccat ctggattatt cattgtcccg caggtgcttc ttttaaactc
     2101 cccgc
//
LOCUS       DAOMYG010000509         2104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_20439_length_2104_cov_6.854075, whole genome shotgun sequence.
ACCESSION   DAOMYG010000509 DAOMYG010000000
VERSION     DAOMYG010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20439_length_2104_cov_6.854075"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PK610_13135"
     CDS             <1..776
                     /locus_tag="PK610_13135"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36932.1"
                     /translation="THVVNANAEYFLTGYHAEDFRSEIKYKFLKKGEKFISSELCAMQ
                     NSPALNTTYTNSNHYFFSGIFLPVVSVSADVNIHFWKDKLAIGSFFVHTTNGMYFRES
                     EFIQDSSSAIAYGGKVSFASNIGKHWRFKAEGIVQQTENENVFRIPGFIGWGSIFYSG
                     TAWSMRFNTGVSTRYYSGFYGNAFVPALNIYQLQNTQLTGNYPLIDAFFEAEISTVRI
                     FISVDHILDGVLGKEFFTAPGYLMPLRTIRFGLRWKFIN"
     gene            complement(779..1759)
                     /locus_tag="PK610_13140"
     CDS             complement(779..1759)
                     /locus_tag="PK610_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36933.1"
                     /translation="MNNDYPTYHNHDIEWFLRNKDFDALTEGEQSFVLTQIDSPEEYA
                     EMRHTLSNIRMSFGTEEVLEPELAVKDTLFAEFDRVHGKQTAGSGRTSWWNTLFPQNK
                     SLFFSPGFQLAGLALIIVGLSFLFTSNNDKNENIALHKNENVVHPTTIEEASPLESVA
                     GGSEDKSEERTSPSPAVSHDLDNNHSSGEMLKATEKEAAREMVVDIQLNDEYKSTVMD
                     SVSSFAFAKGNSSRSENLSQNTQSTAKTDDAVFLLETSVEQKKAVTSDSRKKTSAKES
                     YTKAEEGASSTKTASTGVEQEKSGKDKNESTIGVNAAKNPELLELLFTAL"
     gene            complement(1752..>2104)
                     /locus_tag="PK610_13145"
     CDS             complement(1752..>2104)
                     /locus_tag="PK610_13145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ36934.1"
                     /translation="SVANNMCKNEYRKQEVRKNTVNMGHENFAVADTSADSSRAIDHG
                     KFQEMLQVELDKLEEIQRTTFVLRFKMDMSIKEIAEITEASEGTVKSRLFYTLKKLSE
                     KLKMFDPKLSNAYE"
BASE COUNT          543 a          403 c          415 g          743 t
ORIGIN      
        1 ctactcatgt agtcaatgca aatgcagaat attttcttac cggttatcat gccgaggatt
       61 ttaggtcgga aataaagtat aaatttttaa agaagggaga aaaattcatt tcatcagaac
      121 tttgcgcaat gcaaaattca cctgcactaa atactactta caccaattca aatcattatt
      181 tcttttccgg tatttttctt cctgtagttt ctgtttctgc ggatgtaaat atccattttt
      241 ggaaagataa attggcaata ggttcatttt ttgtgcatac aacaaatgga atgtatttca
      301 gagaaagtga attcattcag gattcaagtt ctgcaattgc ctatggtggt aaggtaagtt
      361 ttgcctcaaa tatcggaaag cactggcggt ttaaggcaga aggtattgta cagcaaactg
      421 aaaacgaaaa cgtattcaga attcctggtt ttatcggatg gggaagtatt ttctattccg
      481 gtacagcctg gtcaatgaga ttcaatacgg gtgtcagcac acgttattat tccggtttct
      541 acggtaatgc ttttgttcct gctttgaata tttatcagct tcaaaatact caacttaccg
      601 gaaattaccc actaatcgat gccttttttg aagccgaaat cagtacggtt agaatcttta
      661 tttctgtaga tcacattcta gatggagttc taggaaaaga attctttact gctcctgggt
      721 atcttatgcc tttgcggact atacgttttg ggctacgttg gaagtttatc aattaagttt
      781 atagtgctgt aaacaacaac tctagtaact cagggttttt tgcagcgtta actccaattg
      841 tactttcgtt tttatcctta cccgacttct cctgttcaac gccggtacta gctgtttttg
      901 ttgatgaagc gccttcttct gctttagtat aggattcctt tgcagaagtt ttctttctgg
      961 aatcggatgt aaccgctttc ttttgttcaa cacttgtttc cagcaggaaa actgcatcat
     1021 ctgtcttggc agtagattgg gtgttttgac ttaagttttc gcttctggaa ctgttacctt
     1081 tcgcaaatgc aaatgaactt accgaatcca taacggtaga tttgtattcg tcatttagtt
     1141 gaatgtcgac gaccatctct cttgctgctt ccttttcagt agcttttaac atttctccac
     1201 ttgaatgatt gttatccaaa tcatgggata ctgcagggga aggtgaggtc ctttcttctg
     1261 atttgtcttc tgaaccgcct gcaactgatt cgagcggact agcctcttca attgtggttg
     1321 gatgcacaac attttcgttt ttgtgcaaag cgatattttc gtttttgtcg ttgtttgatg
     1381 tgaatagaaa gcttagtcca actatgatta gtgccagacc cgcaagttga aaaccgggac
     1441 taaagaacaa tgatttgttt tgagggaaaa gcgtattcca ccaggatgtt ctcccactgc
     1501 ctgcggtttg ttttccatga acacgatcaa attcagcaaa taaggtgtct ttcacagcta
     1561 attcaggttc caaaacttct tcagttccaa agctcatcct gatattggaa agggtatggc
     1621 gcatttcagc atactcttca ggcgaatcga tttgtgtaag tacaaacgat tgctctcctt
     1681 cagttagtgc atcgaaatcc ttattccgaa gaaaccattc gatgtcgtga ttgtgatatg
     1741 tagggtaatc gttattcata agcgttgctc aatttggggt cgaacatttt taatttctcg
     1801 gatagttttt ttaaggtgta gaataaacgc gattttacag ttccttctga ggcttctgta
     1861 atctctgcaa tctccttaat cgacatgtcc attttaaatc gtaatacgaa tgtggtgcgt
     1921 tgtatttcct cgagtttatc taattcaacc tgtaacatct cctggaattt tccatgatcg
     1981 atggcgcgtg aactatcggc tgatgtatct gcaactgcaa agttttcgtg tcccatattt
     2041 acggtgttct tgcgcacttc ttgttttcgg tattcgtttt tacacatgtt attggctaca
     2101 gaat
//
LOCUS       DAOMYG010000510         2101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_20486_length_2101_cov_8.086022, whole genome shotgun sequence.
ACCESSION   DAOMYG010000510 DAOMYG010000000
VERSION     DAOMYG010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20486_length_2101_cov_8.086022"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..>2101)
                     /locus_tag="PK610_13150"
     CDS             complement(107..>2101)
                     /locus_tag="PK610_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRJ36935.1"
                     /translation="NYQISSDQKGVVLKFNRSSFSYAFASFTQLLMQYRSGNLVDFPT
                     ALVVDQPRFSWRGMHLDVSRHFFPVSFIKRYIDILSLYKMNVFHWHLTDDQGWRIEIK
                     KYPRLTSIGGWRNGSMLGHYNEQRFDTVQYGGFYSQDEIREVVAYAEQRGITVVPEIE
                     MPGHAMAALAAYPEYSCNRGKFEVGKTWGVYDDVFCTRDSVFGFLEEVLSEVITLFPS
                     RYIHIGGDEVPKTRWKACSHCQQRMKDEKLKDEHELQSYFIRRIERFVNAKGRQIIGW
                     DEILEGGLAPNAAVMSWRGEEGGIAAANENHYAVMTPGSHCYFDHYQSFPAGEPIAIG
                     GLTTVQKVYSYEPIPSQLKESKHKFILGAQGNVWTEYINTPADVEYMVLPRMLALSEV
                     LWSSRENRNLAAFLVKIQVHFSWFKNSGINFSESVYKTTLRVHPAPNGILGEVLGETS
                     LGKLSYSMISMYPVSGMNTVSDTSIYYTKPFSITKDSRIQTWLALFSGEKKLISEQKV
                     FVHKASSINVTMQPQPSKHYKGFGSFTLVDGIFAQLPRINDQWLAWSGEDVVLTIDLG
                     GEETIEEVTLGTLEDHTNWIWLPEKIELEVSDRGEQFKKLGEYPGKMELKGKREFHIR
                     FPVVNTRYIRLNIKNPGKIPGGFPGEGKDCWMFFDEIMVR"
BASE COUNT          623 a          496 c          389 g          593 t
ORIGIN      
        1 attcctccaa ggcaactgaa cctgcagcaa gaccggtcaa tccgatcgct gagtttttca
       61 aaaattttct tctgtcgctc atgaggaaaa ataaatcaat tcagaattac cgcaccatga
      121 tttcatcaaa aaacatccag cagtccttcc cttctcccgg gaaacctcct ggaatctttc
      181 cgggattttt gatgttcaat cggatatagc gcgtattgac aaccggaaaa cgaatatgga
      241 attcgcgttt gccctttaat tccatcttac cgggatactc ccctaatttt ttaaactgtt
      301 cacctcgatc agaaacttcc agttctattt tttccggcaa ccaaatccag ttggtatgat
      361 cctccaatgt accaagggta acttcttcta tggtttcttc gccgccgagg tcaatggtaa
      421 gaacaacatc ctcacccgac catgccagcc attgatcatt tatccttgga agctgagcga
      481 aaattccgtc gacaagtgta aaacttccaa agcctttgta atgttttgat ggttgaggct
      541 gcatggttac atttatcgaa cttgctttgt ggacaaaaac cttttgttca gaaatcaact
      601 tcttctcacc tgaaaacaaa gccaaccatg tttgaatacg agagtctttc gttatcgaaa
      661 atggttttgt ataataaatt gatgtatctg aaaccgtatt cattcctgaa acaggataca
      721 tggaaatcat agaataactc aatttaccta atgaagtttc acctaaaacc tcacccaata
      781 ttccatttgg agccggatgc actcgaagtg ttgttttata aaccgactca gaaaaattaa
      841 ttcccgaatt tttgaaccag gaaaaatgaa cctgaatctt taccaaaaat gccgcaagat
      901 ttcggttttc ccgactcgac caaagcactt ctgacaaggc cagcattcgt ggaagaacca
      961 tgtattccac atcagcaggg gtattgatat attcggtcca gacatttccc tgcgcaccca
     1021 aaataaattt atgttttgat tctttcaact gagagggaat gggttcgtat gaatacactt
     1081 tctgaacagt tgttaatcca ccaatcgcta tgggttcacc ggcaggaaag gactggtagt
     1141 ggtcgaaata acaatgcgaa ccgggtgtca taacggcata gtgattctca ttagcagcag
     1201 caattccccc ttcttcacct ctccagctca tcactgctgc attgggtgca agaccacctt
     1261 caagaatttc atcccagccg atgatctgtc gcccttttgc atttacaaac ctttcaattc
     1321 ttcggataaa ataactctgc aattcgtgtt cgtctttgag tttttcatcc ttcatccgtt
     1381 gctgacaatg actgcaggct ttccatcttg ttttcggaac ctcatcaccg ccgatatgaa
     1441 tataccggga aggaaaaagt gtgatcactt cagacaacac ctcttcgagg aaaccaaaaa
     1501 cagaatccct ggtgcaaaac acatcatcat acacacccca ggtttttcca acctcaaatt
     1561 ttccgcgatt gcaggaatac tcgggatatg ccgcaagagc agccatagca tgtccgggca
     1621 tttcaatttc aggaacaaca gtaattcccc gttgctctgc ataagcaacc acttcacgga
     1681 tttcatcctg tgaataaaat ccaccatact gaacagtatc aaaacgctgt tcattgtaat
     1741 gtccaagcat ggaaccgttt ctccatccgc cgatggaagt gagtcgtgga tattttttta
     1801 tttcaattct ccagccctga tcgtcggtga ggtgccagtg aaaaacattc attttataca
     1861 gagaaagaat atcgatgtat cgcttaatga aagaaacagg gaaaaagtgc cgtgaaacat
     1921 ccaggtgcat tcctctccag gaaaaccgtg gctgatccac tacaagagcg gtgggaaaat
     1981 caaccagatt acctgagcgg tattgcatta atagttgcgt aaaactggca aatgcgtagg
     2041 aaaaactact acggttaaat tttaagacaa cccccttttg atcggatgaa atttgatagt
     2101 t
//
LOCUS       DAOMYG010000511         2095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_20569_length_2095_cov_9.361275, whole genome shotgun sequence.
ACCESSION   DAOMYG010000511 DAOMYG010000000
VERSION     DAOMYG010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20569_length_2095_cov_9.361275"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..634
                     /locus_tag="PK610_13155"
     CDS             128..634
                     /locus_tag="PK610_13155"
                     /inference="COORDINATES: protein motif:HMM:NF022118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HRJ36936.1"
                     /translation="MEDPIVNKVANSGLISLDLSELLPQAEMVQLDIADQLWQGMVLK
                     EKDFRDWIAATNWSDYSGKYVCLFCSADAIVPAWAYMLLSSSLQPYAAGVFFGSPHEM
                     EEEVLLNAIRSLNEDAFRDARVVVKGCGDIPNQERMLVALTLKLQPIVKSLMFGEPCS
                     TVPVYKRK"
     gene            complement(637..1008)
                     /locus_tag="PK610_13160"
     CDS             complement(637..1008)
                     /locus_tag="PK610_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36937.1"
                     /translation="MTPSTITLDRRLSEVFGEELIIRWNNVNPENISYHQYELSEGFL
                     LINETDLPEHASTEVLESYDPVNFPSDELDLFTIGAFNPLRYNVHRSYSGRKYYRIGK
                     SGKVLVLRSGEELRQDFLSVA"
     gene            1191..1934
                     /locus_tag="PK610_13165"
     CDS             1191..1934
                     /locus_tag="PK610_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36938.1"
                     /translation="MRSIALLLFLICFALQTNAHAEVPYEFGSSSTLISDAGKNVSNP
                     QHWEETAYELSDPKPLFGLSASSATLTYYNDRLCAIAYEFPGNHFESLRQVLNDEYGD
                     PWSYDSTASGRSGYWNSTEKMVSMSDMGSYVLLTFYDDTQKEFHFGDLMRGSLFWVIV
                     SIVGLFLLYYVVAWLLTSYCEKCKSFGMRYKGVRYDNYTNYARTAGFQILQRSEIHPD
                     TIYIYHCKKCKHIRNDRYSGFWKWWRNNN"
BASE COUNT          563 a          401 c          508 g          623 t
ORIGIN      
        1 cagaggtgga aaagaaaact tcagaagttg aaggcgttaa aagcgcaaaa attaatctta
       61 cgtttgatcc tccgtggtcg agagaaatga tgagtgaaga agcgcaactt gaattgggtt
      121 ttttgtgatg gaagatccca ttgtaaataa agtcgcaaat agcggattga tcagtttaga
      181 tttgtcggaa ttgttgccgc aggctgagat ggtgcaattg gatattgctg atcagttgtg
      241 gcagggaatg gtgttgaaag aaaaggattt tcgggattgg attgctgcga caaactggag
      301 cgactattcc ggaaaatacg tttgtctttt ttgcagtgca gatgctattg ttccggcatg
      361 ggcgtatatg ctgttgtcct cttcattaca gccttatgcg gcaggcgttt tttttggatc
      421 gccacatgaa atggaagaag aagtactgtt gaatgccatc cggtcactca atgaagatgc
      481 ttttcgcgat gcgcgtgttg tggtgaaggg atgtggagat atcccgaatc aggaacggat
      541 gctggttgcg ctcacactta aacttcagcc catcgtaaaa agcctgatgt ttggagagcc
      601 ttgctcaaca gttcctgttt ataagaggaa ataaattcag gccacgctca ggaaatcctg
      661 acgcaattct tcacctgaac gaagtacaag aacttttccg gatttcccta tacgatagta
      721 tttcctgcct gaatacgaac ggtgaacatt gtaccgaagt ggattaaacg caccaatggt
      781 aaaaaggtcg agttcgtcgg acggaaaatt caccggatcg taggattcca gcacctcagt
      841 gcttgcgtgt tcaggcaaat ctgtttcgtt gatcagcaaa aaaccttcac tcaattcgta
      901 ttggtggtag ctgatgtttt ccgggttaac gttgttccag cggataatga gttcttcacc
      961 gaaaacttca gataaacgac ggtcgagggt aatggtagat ggtgtcatgt tgtgtgtttt
     1021 atcaggaacg tatttccttt gatttattgt atgcaagtac gaaaacagag atgaagaaat
     1081 cgtaacctga ttcctgcggc gtgtaaagtc tgggttaagt tgcttaagca aatattaccc
     1141 ttgtgtaaaa gcttttttct attttggcat aaactattgt tcctctttct atgcggtcca
     1201 ttgctctatt gttatttctt atttgttttg cacttcaaac aaatgcacat gctgaagtgc
     1261 cctatgaatt tggcagctct tctactttga tctcagatgc aggaaaaaat gtatccaatc
     1321 cccaacattg ggaggaaact gcttatgaac tgtctgatcc gaaacctttg tttggtctat
     1381 ccgcctcttc tgccacatta acgtattaca acgatcgttt gtgtgcaatt gcttatgagt
     1441 tccccggaaa tcattttgaa tcgctccgac aggtgttaaa tgatgaatac ggagacccct
     1501 ggtcgtacga cagcaccgct tccggacgct ccggttattg gaacagtacc gaaaaaatgg
     1561 tgagcatgag cgatatgggt tcgtatgtgt tgcttacgtt ttatgacgat acccaaaagg
     1621 agtttcactt tggcgatctc atgcggggat ccttgttttg ggtcattgta agtattgtcg
     1681 gattgttttt gctctactac gtggttgcgt ggctgctgac ttcctattgc gaaaagtgca
     1741 aatcattcgg tatgcgatac aagggggtgc gttacgataa ttataccaac tacgcccgta
     1801 ccgccggatt tcaaattctc cagcgatcag aaattcatcc cgataccatt tacatctacc
     1861 attgcaaaaa gtgcaaacac atccgcaacg atcggtatag tggattctgg aaatggtgga
     1921 ggaataacaa ttgatttttt ttaggtttga gctaacgggc gttatccaga ccaaagctct
     1981 ggacctgtta taaaagacct aatactgtag tagcttaaca aaagcctata agattttatc
     2041 gcaggtgggg gcagatgacg atgaaagagg gcaggttttg attcagcgtg ccgag
//
LOCUS       DAOMYG010000512         2072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_20885_length_2072_cov_7.013882, whole genome shotgun sequence.
ACCESSION   DAOMYG010000512 DAOMYG010000000
VERSION     DAOMYG010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20885_length_2072_cov_7.013882"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PK610_13170"
     CDS             <1..357
                     /locus_tag="PK610_13170"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRJ36939.1"
                     /translation="VSGLKKRMIERFKSEIPGVSFNGDAEGESLYTVLNVSFPASDNG
                     EMLLFSLDIAGIAASGGSACSSGSNKGSHVLGALNVDPKRTNVRFSFSKYTSTSDIDK
                     AVDAVKRLIGQSLVHS"
     gene            369..1019
                     /gene="upp"
                     /locus_tag="PK610_13175"
     CDS             369..1019
                     /gene="upp"
                     /locus_tag="PK610_13175"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRJ36940.1"
                     /translation="MQVHTLAHQNSVFSSFLAEIRDAHIQKDPLRFRRNLERIGEIIA
                     YELSKHLEFTEKEIETPLGMANCKVLKSQPVIATILRAGLPLHTGVLNYFDRAENAFI
                     SAYRQHHADDTFDIQLEYLASPSIEGKDLVICDPMLATGRSMVAAYKAMLKRGVPRHI
                     HVVSVIGSAEGVEYVRRFMPDNTTIWIGDVDEELTAQAYIVPGLGDAGDLAFGEKI"
     gene            1019..1501
                     /locus_tag="PK610_13180"
     CDS             1019..1501
                     /locus_tag="PK610_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36941.1"
                     /translation="MSILNIVLVFLTASVKFLLAPTLALGLGFQPMTTVLITLVGGVT
                     GILFFYLGADWLMDASAKRRLKKEELLRSQGKTVPEKKIFTKGRRRMIRVKNRFGIVG
                     IAIVTPCIISIPIGCILAARFFKNRTKVLTAMFISATGWAFILTFLNKQVNDLINYLF
                     "
     gene            1501..>2072
                     /locus_tag="PK610_13185"
     CDS             1501..>2072
                     /locus_tag="PK610_13185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HRJ36942.1"
                     /translation="MKGEVTNVFFDLDHTLWDFEANSTEALLELIDLTNIQGILGNEV
                     KSFIQRYKEINHTYWEMYRKGEMEKAELRIGRFRESLSEFGIENPEIHESFAKGYIEI
                     SPEKTNLFPNAKEVLEYLHEKYPMIIITNGFEEVQWRKIKNCGIDKYFKKVVTSEMAG
                     VRKPHPDIFRYAMGISNSIAPRSVMIGDEPS"
BASE COUNT          617 a          383 c          461 g          611 t
ORIGIN      
        1 gtttcaggtc ttaagaaaag gatgattgaa cgattcaaaa gcgaaattcc cggtgtaagt
       61 tttaacggtg atgccgaggg agaatcgctc tatactgttt tgaatgtttc ttttcctgct
      121 tccgacaatg gcgaaatgct tcttttctca cttgatattg caggaattgc agcctccggt
      181 ggaagtgcat gttcgtcggg aagtaacaag ggttctcatg tgctgggagc gctgaatgtt
      241 gatccgaaac gaaccaatgt ccggttttca ttctcaaaat atacaagcac atccgatatt
      301 gacaaagctg ttgatgcagt gaaaagactg atcggacaat cattggttca ttcttaatcg
      361 aaaacatgat tcaggtacac acactcgcac atcagaattc ggtattctca tcttttttag
      421 ctgagatccg ggatgcacat attcaaaaag atcctcttcg ttttcgtcgt aatctggagc
      481 gcattggaga aattatagca tatgagcttt ccaaacatct tgagttcaca gagaaggaaa
      541 tagaaactcc gctgggaatg gcaaattgta aagtattgaa atcgcaacct gtaatcgcaa
      601 cgattcttcg tgcaggattg ccgcttcata cgggtgttct gaattatttt gatcgtgcag
      661 aaaatgcatt tatatctgcc taccggcaac accatgccga tgacacattc gatattcaat
      721 tggagtacct ggcatctcca tctatcgaag gaaaagacct cgtgatctgt gacccaatgc
      781 tggcaacagg acgttcgatg gtggctgctt acaaagcgat gctcaaacga ggagtacccc
      841 gtcatattca tgttgtttcg gttattggca gcgcagaagg tgtggaatat gtgcgaagat
      901 ttatgccgga caataccacc atttggattg gagatgtaga tgaagagtta accgcacaag
      961 cctacatcgt tcccggatta ggtgatgccg gtgatcttgc ttttggggag aaaatctgat
     1021 gagtattctc aatatagttc tcgtctttct taccgcatca gtaaaatttt tgcttgcacc
     1081 aacacttgcg ctgggactcg gattccaacc tatgacaaca gttctcatta cacttgttgg
     1141 aggtgttacc ggaattctgt ttttttattt gggagcagat tggttaatgg atgcttcggc
     1201 aaagcgaaga ttgaaaaaag aagaattgtt acgttctcaa ggcaagacag tacctgaaaa
     1261 aaagattttt acaaaaggcc gaagaagaat gattcgggta aaaaacagat ttggtattgt
     1321 aggtattgca attgttactc cctgtatcat ttccattcca ataggttgta ttctggcagc
     1381 acgttttttt aaaaacagaa ccaaagtcct gacggcaatg tttatttccg ctactggttg
     1441 ggcatttatc ctgacatttt tgaataaaca agtcaacgat ctcatcaatt atcttttttg
     1501 atgaaaggag aagtaacgaa tgtctttttc gacctcgatc atacattgtg ggatttcgaa
     1561 gcgaattcta ctgaggcatt gcttgagttg attgatctga caaacattca aggaattctt
     1621 ggcaatgaag taaaatcatt tatacaacgg tacaaggaga tcaatcacac ttattgggaa
     1681 atgtaccgta aaggtgaaat ggaaaaagct gaattacgaa ttggtcgttt tcgtgagtct
     1741 ttgtctgaat ttggaataga aaatccggaa atacatgaat catttgccaa aggatatatt
     1801 gagatcagtc cggaaaaaac caaccttttt cccaatgcaa aagaggtact ggaatatctg
     1861 catgaaaaat acccgatgat tatcatcacc aacggttttg aggaagtgca gtggcgaaaa
     1921 ataaaaaatt gcggaatcga taagtatttt aaaaaggtag tcacctctga gatggcaggt
     1981 gtgcggaaac ctcatcctga tatttttcgg tatgcaatgg gaatttcaaa ttctattgcc
     2041 ccgcggtcag ttatgattgg tgatgaacct tc
//
LOCUS       DAOMYG010000513         2045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_21303_length_2045_cov_9.382412, whole genome shotgun sequence.
ACCESSION   DAOMYG010000513 DAOMYG010000000
VERSION     DAOMYG010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21303_length_2045_cov_9.382412"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /gene="menD"
                     /locus_tag="PK610_13190"
     CDS             complement(<1..1034)
                     /gene="menD"
                     /locus_tag="PK610_13190"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HRJ36943.1"
                     /translation="MYSDKAGVTHLVETLIQKGLREVVLSPGSRNAPLSLSFHHHPEI
                     ELIVIPDERSAAYFALGMAQQLRRPVAVVCTSGTAALNYAPAIAEAYYQKVPLLILTA
                     DRPIAWVDQADGQTIRQRDLFLNYVKHGYEIPGDGKTEEELWFIRRIVSQAYDQTLLH
                     GEGPVHINIPLSEPLYGRSKELPQLRPIDTLVPESSLSEDQLMSLAERWNNSERKLIL
                     TGLMEPHPALEKMLEQIADDPSVTVLTETTSNLHSERFNGCIDRIITSIHDGEKIDFH
                     PDLLITFGGPVISKKVKVFIRKNTPKEHWHIDPSEMHLDTYQCLTHTIPLSPAILAVA
                     EKGRRVLALAV"
     gene            complement(1105..1851)
                     /locus_tag="PK610_13195"
     CDS             complement(1105..1851)
                     /locus_tag="PK610_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36944.1"
                     /translation="MLCSLSGIHNISAQQSKKSDTLVLHKEKYPNGKISAYSYILNST
                     GNGKAVCYDPQGKIMYQGEVSRNHGHRSVYFQHHPNKVVSKIELSSAPDGGIQWYRTY
                     LFFNEQGVLVNKIEDNWDDRVTVLTDPGPGKKEEKPAPKQEVVECAVIYSNEAWLINQ
                     TPHPVHVTWIARQETITKTIQPRDTVFIANLIQAQFFADPYKSASVVVKKAKGKPSKG
                     VRYELIPLSEKQEGKEKKRYYYRVLAVLTE"
BASE COUNT          524 a          417 c          491 g          613 t
ORIGIN      
        1 acggcaaggg cgagtacgcg acgacctttt tcggctacgg cgagaatggc aggactgagt
       61 ggaatggtat gtgttaaaca ttggtaagta tccaggtgca tttcagaagg atcaatatgc
      121 cagtgttctt tgggagtgtt tttgcgaata aagactttta cttttttcga gattacaggt
      181 ccgccaaagg tgatgagcag atcgggatgg aaatctattt tctcgccatc atgaatggaa
      241 gtaatgatac gatcgatgca gccattgaaa cgttcactgt gaagatttga tgtggtctct
      301 gtcaggactg ttacagatgg atcatctgca atctgctcaa gcattttttc caacgcagga
      361 tgtggttcca tcagtccggt aagaatcagt tttcgttcgg aattgttcca gcgttctgca
      421 agcgacatca actgatcttc agataaggaa gattcaggaa ccaatgtgtc gatcggacga
      481 agttgtggta attccttgct tcttccgtag agaggttcgc ttaagggaat attgatgtgt
      541 acaggacctt caccgtgcag cagggtttga tcgtaagcct gtgatacaat tctgcggatg
      601 aaccaaagct cttcttccgt ttttccgtcg ccgggaattt cgtagccgtg tttaacgtag
      661 ttgaggaata gatcgcgctg acgaatggtt tgaccatcgg cttgatccac ccaggcaata
      721 ggacgatctg cagtgagtat cagtagcggt actttttggt aatacgcctc tgcaatagca
      781 ggagcatagt taagtgcagc agttcctgaa gtacacacta ctgcaaccgg acggcgcaac
      841 tgttgggcca ttcccaaagc gaaataggcg gcactgcgtt cgtcgggaat gacgatcagt
      901 tcaatttcag gatgatggtg aaaactcagc gaaagaggag cgtttctgga acccggactc
      961 aatacaactt ccctaagtcc tttctggata agggtttcaa cgagatgtgt tactccggct
     1021 ttgtcgctgt acatgggggt aaaagtaatc aaaactacct tttaccctaa caagcagaat
     1081 gacgcttggt tgcaggtatg cggattattc ggtcaatacc gccagcaccc ggtaataata
     1141 ccgtttcttt tccttgcctt cctgtttttc cgataatgga atcaactcat agcgaactcc
     1201 ttttgaaggc tttccttttg cttttttaac cactacagat gcgcttttgt agggatcggc
     1261 aaagaattgt gcctgaatta aattggcaat gaaaacagtg tcgcgtggtt gaatcgtttt
     1321 ggtaatggtt tcctgacggg caatccatgt gacatgaacg ggatgcggag tttggttgat
     1381 cagccaggct tcgttcgaat agatgactgc gcattctacc acctcttgtt ttggagcagg
     1441 tttttcttcc ttttttcccg gacccggatc tgtcagcacc gtaacgcggt catcccaatt
     1501 gtcttcgatc ttatttacca atacaccctg ttcattgaaa aaaagatagg tgcggtacca
     1561 ttgaattccg ccgtcgggag ctgaggataa ttcaatttta ctgaccacct tattggggtg
     1621 atgctggaaa taaacggagc gatgtccgtg gttgcggctc acttctccct ggtacatgat
     1681 ttttccttgg ggatcgtaac agactgcctt tccgtttccg gtgctgttga ggatataact
     1741 gtaagcagaa atttttccgt tcgggtattt ctctttgtgc aaaacgaggg tatcgctttt
     1801 ttttgactgc tgagcagaaa tgttatgaat tcctgataat gaacaaagca tcagaaataa
     1861 aagtgtcggt attttcgaaa aggttatcat aagtgccgga aagatacctt ggaaattcaa
     1921 aatttatgcc aaataatgaa tctgatattt tatgagtaat aaattatcgt tcttgtacct
     1981 tagatgtaat cgtttttccg gattaccgca aacctatttt cgtaatcaaa aaatcgatcg
     2041 tcctc
//
LOCUS       DAOMYG010000514         2028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_21579_length_2028_cov_7.551445, whole genome shotgun sequence.
ACCESSION   DAOMYG010000514 DAOMYG010000000
VERSION     DAOMYG010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21579_length_2028_cov_7.551445"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PK610_13200"
     CDS             complement(<1..847)
                     /locus_tag="PK610_13200"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HRJ36945.1"
                     /translation="MKKFRLLFAALVISTGLFFQSCDKVDQPYYDTPVCPAVTFPDTN
                     GTTRNILFEEFTGHTCGNCPSGAYDLRTIWKPMYDDRLIIVSVHAGVFATPDIPAGYY
                     ADYRTEASVEYDAFFGVTGYPSGMVNRVPYASQYVVGKVNWGNAFAVEAAKPLEIDLQ
                     MYVEHDELTDPYSVCLHMHSKFLTSKNGRFNVVGYFVEDSIISKQKNYPGAQGDPDYL
                     VPQDPNYVHRHMLRDNIGGTWGVNMWSGNAPAGKDTTVSLKYNFQPRDTVDFSHRKHH
                     YYFVGY"
     gene            complement(851..>2028)
                     /locus_tag="PK610_13205"
     CDS             complement(851..>2028)
                     /locus_tag="PK610_13205"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HRJ36946.1"
                     /translation="GKQRLFWRLADGVVRGADAEVNLNEMLDSVMGRMKTKFILGASF
                     VSRYQNPADVNYVMPANVGSFATRLNIIRDKWNVSGEYVYKINDPSADNKFIYKNGQG
                     LLLQFNYATKGFGLSLSGKYIDNMSFRSDRYEGFTNVFINFNPALTRPHTYNLAATLY
                     PYACQPTGEIAFQGELNYKFQKGTLLGGKYGANLAVNYSYANGLDTTTFSPEKDSARM
                     GYSSRFFGPGSNKYFRDFNIEFRKKVSDKFKFVLMYIYLEYDMEIVQNLIGKGTIYSH
                     IGVADLNYKIDKKNNIRVEVQHLYTQQDKGSWATVLVEYTRSPHWFIGALSQYNYGNE
                     VEAQRVNYPYLTVGYIQNSTRITMGYGKQREGLFCVGGVCRAVPASNGFTLSITSSF"
BASE COUNT          603 a          460 c          370 g          595 t
ORIGIN      
        1 catatcctac aaaatagtaa tgatgtttcc gatgtgaaaa atcaactgtg tcacgaggct
       61 gaaaattata tttcagactt actgttgtgt cttttcctgc aggagcattt ccgctccaca
      121 tatttacacc ccatgtaccc ccaatattat cacgaagcat atggcggtga acataattag
      181 gatcctgcgg caccagataa tcaggatcac cctgagctcc gggataattt ttttgcttgg
      241 agataatgct gtcctcaaca aaatatccaa caacattaaa cctgccattc tttgaagtca
      301 agaactttga atgcatgtgc agacaaacag aatacggatc cgttaattca tcatgctcaa
      361 catacatctg aaggtcgatt tcaagaggtt tagccgcttc aacagcgaat gcgttacccc
      421 agtttacttt acctaccaca tactgactgg catatggcac acgatttacc attcccgaag
      481 gataaccggt tacgccaaaa aacgcatcgt attcgaccga tgcttctgta cgataatctg
      541 cataataacc cgccggaata tcaggggttg caaacacacc tgcatgcaca gaaacaatta
      601 ttaaacgatc gtcatacatt ggcttccata tggttcttaa gtcataggca ccgctcggac
      661 aattaccaca tgtatgacct gtaaactctt caaaaagaat atttcttgtg gttccgtttg
      721 tatccggaaa cgtaacagca ggacaaacag gcgtgtcata ataaggttga tcaactttat
      781 cgcacgactg aaaaaacaat cctgttgata ttaccaaagc tgcaaatagt aagcgaaact
      841 tcttcataga tcaaaaacta ctggtgattg ataatgtaaa tccgttcgat gcaggcacag
      901 cacgacaaac acctcctaca caaaatagtc cttctcgttg ttttccatat cccattgtta
      961 tcctggtcga attctgaata tatccaacgg taagatacgg ataatttacc ctttgtgctt
     1021 caacctcatt gccataattg tattgcgata aagctcctat aaaccaatga ggtgaacggg
     1081 tatattctac cagaacagtg gcccagcttc ctttatcctg ctgcgtgtat aaatgttgaa
     1141 cctcaactct gatgttgttc tttttatcaa tcttatagtt gagatcagca actccaatgt
     1201 gcgaatagat tgttcccttt cctataagat tctgtacaat ctccatatcg tattcgagat
     1261 agatgtacat gagtacaaat ttgaatttgt cgctcacttt ttttctgaac tcaatattga
     1321 agtctctgaa atacttgttg gatccgggcc cgaaaaaccg tgagctatac cccatgcgcg
     1381 cagaatcttt ttccggacta aatgtcgttg tgtccaagcc gttggcataa gaataattca
     1441 ccgccaggtt tgcgccatat ttacctccaa gtaatgttcc tttctgaaac ttataattca
     1501 actcgccctg aaatgcaatt tctccggttg gctggcatgc ataggggtac agagttgctg
     1561 caagattata tgtgtggggg cgggtaagtg caggattaaa attgataaat acattagtaa
     1621 aaccctcata acgatcagag cggaaactca tgttgtcgat gtatttaccg gaaagagaaa
     1681 gtccaaaccc tttggttgcg taattaaact gcaacaataa gccctgcccg tttttgtaaa
     1741 tgaatttgtt atcggcagat gggtcgttta ttttgtaaac atattctccc gaaacattcc
     1801 acttatcccg aatgatattt aatcgtgtgg caaacgatcc gacattggca ggcataacgt
     1861 aatttacatc tgcagggttt tgatacctgc tgacaaaact tgcccccaga ataaatttgg
     1921 ttttcattct tcccattact gagtcgagca tttcattcag atttacctct gcatcagcac
     1981 cacgaacaac accatctgca agcctccaga ataaacgttg ctttccgt
//
LOCUS       DAOMYG010000515         2028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_21583_length_2028_cov_7.020781, whole genome shotgun sequence.
ACCESSION   DAOMYG010000515 DAOMYG010000000
VERSION     DAOMYG010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21583_length_2028_cov_7.020781"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PK610_13210"
     CDS             complement(<1..427)
                     /locus_tag="PK610_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36947.1"
                     /translation="MAITTIDSAGFEKLFRQFIRESFNGKRTSASGKRISDATANNYT
                     NTFRILLRFQRERNFQLCLHLVHRVNQKDIKTLNVYWRNFMRELHLFLYHDNGYYDNY
                     AGSILKCLKVFYNYLLKEKGMPIGQFHKRIFVPKEEVEFV"
     gene            complement(585..863)
                     /locus_tag="PK610_13215"
     CDS             complement(585..863)
                     /locus_tag="PK610_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36948.1"
                     /translation="METEKKLKLEESDEFSYLNPHPLKITNEEYMAVYFRTYRYEYFL
                     EKGTPSEKKLARTFFLQLIAYWEMRVASTSDCQERWVKRMKEREGVLG"
     gene            complement(868..>2028)
                     /locus_tag="PK610_13220"
     CDS             complement(868..>2028)
                     /locus_tag="PK610_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRJ36949.1"
                     /translation="INIYLTKDDFRKITSGKRLSEELMEIKTRIEMTQQRAQEACKGM
                     IRFDKEEFLKRFNGESVKQENVVYSIRVAFDRSKAKEGIKIATRLCYQTSLIAINRYL
                     VNRKLSVDAVEITKVNVDFLKGLHAYLLDEGLSFATIGIYMRTFRAVINEAMALGKIT
                     PQDYPFGRRKYVPPVAKSTKDVLAKNELKAMIELKLEPLSLECFSRDWFLMSYLCYGM
                     NIGDLLNLKNKNLRQGGFHFIREKTKTTSGGGPDHIRVDFGQSWKDLIWEIIERQRSN
                     RTEADEYLFPYYESGMDERRRMRTKLIMIRKVNQHLPKVCKLAGITKKVSTQTARHSF
                     ASNLYQGDVSIAEIQQMLGHTSPRTTENYLHSLRTDRTNKAIENLLSLDDQK"
BASE COUNT          527 a          445 c          406 g          650 t
ORIGIN      
        1 ccacaaattc cacttcctcc tttggaacaa atatgcgctt atggaattgt ccaatcggca
       61 ttcctttctc ttttagcaga taattgtaaa acacttttag gcacttgagg atagaacctg
      121 cataattgtc gtaataacca ttatcatgat acaaaaacag atgaagttcc ctcatgaaat
      181 ttcgccaata cacgttgagc gttttgatgt ctttttggtt cactctatga acaagatgga
      241 gacacaactg aaaattcctt tcgcgttgaa accgaagcag aattcgaaat gtgttggtgt
      301 aattgtttgc agttgcatca gaaatacgct ttccggatgc gctggtccgt tttccgttaa
      361 aggactccct gatgaattgc cgaaaaagtt tttcaaatcc tgctgaatcg atcgttgtaa
      421 tggccatgat tttttagtgg cttatacgaa gcagataatg ccgacagttt ttcaaaatcc
      481 ggttttattc caaaggatga aaacaaatta caactgtttc tatctgatgg gagggtgttc
      541 aataggcaag gttatcattt catagcataa acttttggta attattatcc caacactccc
      601 tctcgctcct tcatgcgttt gacccatctt tcctggcaat cgctggtgga tgcaacacgc
      661 atttcccaat aggcgatcaa ttgaaggaaa aacgttcttg ccagtttttt ttcggatggt
      721 gttccttttt ctaaaaagta ctcgtaacga taggtgcgaa aataaaccgc catgtattct
      781 tcgtttgtaa tttttaaagg atgaggattg agataggaga attcatcgct ttcctcaagt
      841 ttcagttttt tttcggtttc catggcttca tttttggtcg tcaagtgaaa gcagattttc
      901 aatggccttg ttggtgcggt cggttcgtag gctatggagg tagttctcgg tggtgcgcgg
      961 actcgtatgc cccagcattt gttggatctc tgctatgctg acatcgcctt gatagaggtt
     1021 tgatgcaaag gaatgccgcg ctgtttgtgt agatactttt ttagtgattc cggccagttt
     1081 gcagaccttt ggtaaatgtt ggttcacctt acggatcata atgagtttcg tccgcattcg
     1141 ccggcgctcg tccattcccg attcgtaata tggaaaaagg tattcatccg cttcagttcg
     1201 attggagcgt tgccgttcaa tgatttccca aatgagatct ttccaggact gaccaaaatc
     1261 aacacgaatg tgatccggcc ctccgccgga ggtggtcttt gttttctctc ggataaaatg
     1321 aaaacctccc tgacgtaaat ttttgttctt gaggttgagc aaatcgccta tgttcattcc
     1381 atagcacaaa tacgacatca gaaaccagtc ccttgaaaag cattccagcg agagcggttc
     1441 cagtttcagt tcaatcatgg ccttgagttc attttttgcc aatacatctt tggtactctt
     1501 tgcaaccgga ggaacgtatt tacgccgccc aaaaggataa tcctgcggcg taattttccc
     1561 caaagccatg gcctcattga tcaccgcacg aaatgtccgc atgtaaatac caattgtagc
     1621 aaatgaaagt ccttcatcaa gtaaatacgc atgcagccct ttaagaaaat caacattaac
     1681 cttagtaatc tccactgcat caactgatag ttttcgattt accagatacc tgtttatggc
     1741 aattagtgat gtctggtaac acaagcgtgt tgcaatttta atcccctctt tggctttgga
     1801 acgatcaaaa gctaccctga tactataaac cacattttct tgctttacgc tttcgccatt
     1861 gaaccttttg aggaattcct ccttatcaaa gcggatcatt cctttacacg cctcctgagc
     1921 ccgttgctga gtcatttcaa tccgtgtctt gatctccatc aattcttccg acagccgttt
     1981 accggaagtt attttgcgaa aatcatcttt ggtcaaatag atgttgat
//
LOCUS       DAOMYG010000516         2020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_21711_length_2020_cov_6.276845, whole genome shotgun sequence.
ACCESSION   DAOMYG010000516 DAOMYG010000000
VERSION     DAOMYG010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21711_length_2020_cov_6.276845"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /locus_tag="PK610_13225"
     CDS             complement(<1..1524)
                     /locus_tag="PK610_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36950.1"
                     /translation="MKSPLSLIGKSSFSILLIALFALIACNRDTVKQIQIDPAFGQYI
                     SAYTSGIVSNQSSVVIQLVADYPNPSDPQTPVADELFELSPSVQGKAYWTNNRTIEFK
                     PDQPLQQNTEYVVTFHLSKIMEVESKLGDFVFQIKTREQSIEVYTEGLSTYDPNNLKR
                     QKLEGQFVLADWSDTAAIHKSLRANQDGKDLKIDWMHGEVNIHRFVVQNIARKEKRSA
                     ILLTWNGEAVGASGSGEKKIEVPALGDFKITDVYVNQTPEQFIVVHYSDPLKADQNFN
                     GLVTIQDSYNLSFVVDGHKLMVYPGRHLTGPKLLSVYRGVKNIMGYSCSDEKTVEVEF
                     EELKPQVRLVGEGNIIPTTDKGMLFPFEAVNLKAVDVKVTRIFQNNIVQFLQVNSLDG
                     SSQLKRVGREIFKKRVELDPEGKLDLGQWNRFSVDIGALVRSEPGAIYHISLGFNRAY
                     SVYSCDNQDATANQLTELPESGKDKEWTEDGWGSFDYGYYYEDYDDGDYYYDYNYSER
                     "
     gene            1727..>2020
                     /locus_tag="PK610_13230"
     CDS             1727..>2020
                     /locus_tag="PK610_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36951.1"
                     /translation="MVSLRNSAFSILAIVLLSACNGKGESDALSEKNNKETGEVITPL
                     PRQIQNTVAIQRHFSSTIEKDEFIITIRGENYLKAKVCFSIINNAGDTLFVRHS"
BASE COUNT          566 a          447 c          405 g          602 t
ORIGIN      
        1 acgttccgaa taattgtagt cgtaatagta atctccatcg tcgtaatctt cgtagtaata
       61 accgtaatca aaacttcccc aaccatcttc tgtccattct ttatccttcc cgctttcggg
      121 caactccgtc aactgattgg cggtggcatc ctgattatcg caggaataaa ccgaatacgc
      181 ccggttaaaa ccaagagaaa tatgatagat cgcaccgggt tctgaacgta caagcgcgcc
      241 aatatctaca gagaatctgt tccactgacc caaatccaat tttccttcgg gatcaagttc
      301 aacccgcttt ttgaaaattt ctcttccaac tcttttaagt tgactggatc catctaaact
      361 gttgacttga agaaactgaa caatattgtt ctgaaaaatg cgtgtcactt taacgtcaac
      421 agcttttaga tttacagctt caaatgggaa caacattcct ttgtcggttg ttggaatgat
      481 atttccttct cctactaaac ggacttgcgg tttcagctct tcaaattcaa cttccaccgt
      541 tttttcatcc gagcaactgt atcccattat attttttact ccgcgataaa cactcaatag
      601 ttttggtccg gttaaatgtc ttcccggata aaccatgagt ttatgtccat ccacaacaaa
      661 acttaaattg taggaatcct gaatggtaac cagtccatta aaattctgat cggctttgag
      721 gggatcggaa tagtgcacca caataaattg ttcgggtgtt tgattcacat acacatcggt
      781 aatcttaaaa tcacccaagg caggaacttc aatctttttt tcacctgagc ctgatgcacc
      841 gacagcttcc ccattccatg ttagaagaat ggcacttctt ttttctttcc gtgcaatatt
      901 ttgtacaaca aaacgatgaa tgtttacttc accatgcatc caatcaattt ttaagtcttt
      961 accatcctga tttgcacgta atgatttgtg gatagcggcg gtatctgacc aatcggcaag
     1021 tacaaactga ccttcgagct tctgacgttt caggttattg ggatcataag tggacaatcc
     1081 ctcggtgtac acttctattg attgttcgcg ggtttttatt tgaaagacaa aatcgcccaa
     1141 cttactttca acttccatga tcttacttaa atgaaatgtg accacatact ccgtattctg
     1201 ctgcaacggc tggtcgggtt tgaattcgat ggtgcggttg ttggtccagt aggccttacc
     1261 ctgaactgaa ggactcaatt caaaaagctc atcagcaacc ggagtttggg gatcgcttgg
     1321 gttggggtaa tcagcaacaa gctggataac cacagaagac tggttggaaa caattcctga
     1381 agtataagcg gaaatgtact gaccgaatgc gggatcgatc tgaatttgct ttacggtatc
     1441 gcggttacag gcaatcagcg cgaaaagggc gatcaataaa atggagaaag atgatttacc
     1501 gatcagggag aggggggatt tcatggtgcg tgttttaggc agaacaaaca taatctacct
     1561 gaaaccaatt gccaacgggt ttggaaaagt cgtggtaaat aatcgattaa acgctgtcgc
     1621 gccccgagta agtgcaatcc cggcccagcc cttgtcctgt agcacgattt ggtaaccatc
     1681 ccctaggctg ctcccgactt tgtttgattt ttactatttt tagcctatgg tttcgcttag
     1741 aaatagtgct ttttccatcc ttgcaattgt tctgctcagt gcctgtaatg gcaaagggga
     1801 gtcggatgct ttgagtgaaa aaaacaataa ggaaaccggg gaggtcatta ctcctctgcc
     1861 ccgacagatc caaaacacgg tggcaattca gcggcatttt tcatccacta tagagaaaga
     1921 tgaatttata attacaattc gtggtgaaaa ttatttaaaa gccaaagtct gtttttccat
     1981 cataaacaat gccggtgata ctctttttgt cagacacagt
//
LOCUS       DAOMYG010000517         1999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_22047_length_1999_cov_8.084877, whole genome shotgun sequence.
ACCESSION   DAOMYG010000517 DAOMYG010000000
VERSION     DAOMYG010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22047_length_1999_cov_8.084877"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PK610_13235"
     CDS             complement(<1..418)
                     /locus_tag="PK610_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36952.1"
                     /translation="MTPHEFWSWFNENRFRYLFINDEAMEERDQALEDLANQLAEYKS
                     GLFFLIGGQADREEQDLIITSDGQRNLFPMVKELCDAAPKIEKWRVVAFKPALGPDIQ
                     LEYRDILFDPRQLWFIPLVHPDHPEYTGIRIGMQDYT"
     gene            525..1028
                     /locus_tag="PK610_13240"
     CDS             525..1028
                     /locus_tag="PK610_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36953.1"
                     /translation="MKFTSRLKTVFNCSLERAFKTPFLCDVSKVHTGYGLMPRVTYCT
                     DDKDWGNPGAVKKVFVAKSISHKGGFASTDKIIERIENQYWKIEVSDFQTWMLGFTRF
                     VGEWKTTEINPDKIEVTYTYTLHAGNPLLYPFNYLFAKLFWRVYMKRVVKNIRNMIEN
                     EEPYQFS"
     gene            1052..1993
                     /locus_tag="PK610_13245"
     CDS             1052..1993
                     /locus_tag="PK610_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36954.1"
                     /translation="MTTAKFSLIVLFITSLAFSSCTGSEKKSAENKPINSDTTIAKEY
                     EELSEVNEEDALTQLLFPMVLNGSVDNYKIEMEISNFNPGTNEITGKYKYDSQKAYLE
                     LKGKAMGTTWMLEEYSKGKNTGTFYLNIDHKSAMGWWTDEKKSLAVDLASSSRISSVN
                     SFDFEYNNFNPGKGITGSYSTEYYWVNDFHNDPTQPALEIGFNGGYAVFEEQKNGNLK
                     FQLEVICGPTYHFAYAEGIAEKKGKKYIYTSSEYSDETPCEIVFTIDGNIIHAEANQS
                     MGCGFGARAYLSHDFTKFSDKFQMGEGVSLEKLKGLK"
BASE COUNT          642 a          392 c          416 g          549 t
ORIGIN      
        1 gtgtgtaatc ctgcatgccg atgcgaatac cagtgtattc cgggtgatcg gggtgcacaa
       61 ggggaatgaa ccataattga cgtggatcaa agaggatgtc gcggtactcg agttggatgt
      121 cgggacctaa tgcaggtttg aaggcgacca ctctccattt ctcaattttt ggtgcggcat
      181 cgcaaagctc tttcaccatg ggaaaaagat tgcgctggcc atcggatgta ataatgagat
      241 cctgctcctc ccggtcggcc tgacctccga ttaagaagaa cagtcctgac ttatattctg
      301 ccaactgatt ggccaggtct tccaatgcct gatcgcgctc ttccattgct tcatcattga
      361 tgaacagata acggaagcgg ttttcattga accaggacca aaattcgtgt ggtgtcatag
      421 gacagatctt acctgtttcg aaagtaaata attacctcgg cattttcaaa tcaaatctcc
      481 cattggcgtt aaacaaaaca ggttttatca gatatttgtg aacgatgaaa tttacatcac
      541 gtcttaaaac agttttcaat tgcagtcttg aacgtgcatt taaaacacct tttttatgcg
      601 atgtttccaa agtacatacc ggttatggac taatgcccag ggtaacctat tgtacagatg
      661 ataaggattg gggaaatcca ggagctgtaa agaaagtatt tgtggcaaaa tcgatcagtc
      721 ataaaggagg atttgcctca accgataaaa taattgaacg tatcgaaaac caatactgga
      781 aaatagaagt aagcgatttt cagacctgga tgttgggttt tactcggttt gttggcgaat
      841 ggaaaacaac agaaatcaat cccgacaaaa ttgaagttac ctatacttat actcttcatg
      901 ccggtaatcc tttgttgtat ccctttaatt atttatttgc aaaactcttc tggcgtgtct
      961 acatgaaaag ggtggtgaaa aacattcgta acatgattga aaacgaagaa ccgtatcaat
     1021 ttagctgaac tcaaaaaata acagaacttt tatgactact gcaaaattta gcttaatcgt
     1081 attatttatt acttcacttg cattttcatc ctgtactggt agcgaaaaaa agtcggcaga
     1141 aaacaagcct atcaattcgg atactactat tgcaaaagaa tatgaagaac tttctgaagt
     1201 caatgaagaa gatgcgctga cgcaattgct ttttccaatg gtcctcaatg gatcagtgga
     1261 taattacaaa atcgaaatgg aaatttcaaa cttcaaccct ggaactaatg aaataaccgg
     1321 taaatacaaa tacgattccc aaaaagctta tctcgaactt aagggcaaag caatgggaac
     1381 aacctggatg ctggaagaat attcgaaagg gaaaaatacc ggaacattct acctgaatat
     1441 cgatcataaa agcgcaatgg gctggtggac cgatgaaaag aaatccctgg cagtcgatct
     1501 cgcttcatca tctcgtattt cttctgttaa ttcttttgat ttcgaataca acaacttcaa
     1561 tcccggaaaa ggaattaccg gatcgtattc tacagaatat tattgggtca acgattttca
     1621 caatgatcca actcaacctg ctctcgaaat cgggttcaat ggtgggtatg ccgtttttga
     1681 agaacagaaa aacggaaacc tgaaatttca gcttgaagtc atttgcggac caacttacca
     1741 ttttgcctat gccgaaggaa ttgctgaaaa gaaaggcaag aaatatattt ataccagcag
     1801 cgaatacagc gatgaaacac cttgtgagat cgtcttcacc atcgatggaa acatcatcca
     1861 tgccgaagcc aatcaatcaa tgggttgcgg atttggagcg cgtgcctacc ttagtcacga
     1921 tttcaccaaa ttctccgata aattccaaat gggagaagga gtgagtttgg agaagttgaa
     1981 gggtttgaaa taaccaggg
//
LOCUS       DAOMYG010000518         1998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_22067_length_1998_cov_6.941843, whole genome shotgun sequence.
ACCESSION   DAOMYG010000518 DAOMYG010000000
VERSION     DAOMYG010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22067_length_1998_cov_6.941843"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PK610_13250"
     CDS             complement(<1..423)
                     /locus_tag="PK610_13250"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRJ36955.1"
                     /translation="MKRIGVISDTHSFLDEKVFDYFEHCDEIWHAGDIGDLRVTDRLA
                     AFRPLKAVWGNIDDHKARIQFPETNRFLCEGMDVMIHHIAGKPYSYPANVKAMMNPKT
                     PDILVCGHSHILRVEYDKKINMLYINPGAAGVHGFHKVK"
     gene            complement(420..>1998)
                     /locus_tag="PK610_13255"
     CDS             complement(420..>1998)
                     /locus_tag="PK610_13255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRJ36956.1"
                     /translation="TIITLVLLGNYVEHKAVTQTTSALRDLMKLQKTIAKRVKIVNGT
                     ESIEEVVADSVQVDDVLRVNSGDRIPIDGIIIYGEVLVDESMISGESLPVRKRTGDAI
                     TGGTLVADGAFTMKATRVGQGTTLAKIIAMVKEAQLQKPSIQKLGDKISSIFVPVVVA
                     ISVLTFLIAYFAISLSATHALMNAIAVLVISCPCAMGLATPTAVAAAIGRAAKAGILF
                     KGADTIEQLAKVKTVVFDKTGTLTTGKFSIKAIHRIGDCTEEEIKSAIAGLEQHSSHP
                     IAKSLVKELVDSAPMIFSEVNEQKGKGITGRDSKGRIWSFGSALLHHTNANDAEPGIQ
                     LYCDGRPIARIEIGDEIREGAKEMIRDLNASGIRTILLSGDRNSNCEKIANELGIQEW
                     YGERLPDQKLLKIRELNTNHDCAMVGDGINDAPALAAAEIGISLGESSQIAIQSSQVV
                     ILRNDLSGISTVISIARLSLKTIHQNLFWAFFYNVLAIPVAAFGLLSPMIAALSMAFS
                     DIIVVGNSIRLKFRAIR"
BASE COUNT          546 a          447 c          403 g          602 t
ORIGIN      
        1 tttcacttta tgaaatccat gcacacccgc tgctccgggg ttgatgtaga gcatgttgat
       61 cttcttatcg tattcaaccc gaaggatgtg ggaatgtccg caaacgagga tgtcgggtgt
      121 tttgggattc atcatggcct tcacattcgc aggataggag tagggcttac cggcaatgtg
      181 gtgaatcatg acatccattc cttcgcataa aaaacgattg gtttctggaa actgaatccg
      241 tgctttgtga tcgtcgatgt ttccccagac agctttcaag ggtctaaacg ctgccagtcg
      301 gtcggtcaca cgtaaatcac caatatctcc ggcgtgccag atttcatcac aatgttcaaa
      361 ataatcgaac actttttcat cgagaaaact atgcgtatcg ctgataactc caatgcgttt
      421 catctgattg cccggaactt taaacgaatc gaattcccaa ccacaataat atcggaaaag
      481 gccatggata atgcagcaat cattggactt agcagtccga aagctgcaac aggaatagca
      541 agaacattat aaaagaatgc ccagaataaa ttctggtgaa tagtttttaa cgacaatcgt
      601 gcaatggaaa ttacagtaga aattccggaa agatcatttc taaggattac cacttgcgaa
      661 gattgaattg caatctgaga agattctccc agggaaatac caatttcggc tgcagcaagg
      721 gcaggagcgt cattgatccc atcaccaacc atggcgcaat catgatttgt atttagttca
      781 cgaattttta ataatttctg atccggtaat cgttcaccgt accattcctg aattccaagt
      841 tcattggcaa ttttctcgca attggagttt ctgtcgccac tcagcaaaat tgttcgtatt
      901 ccggatgcat tcagatctcg gatcatctct tttgcacctt cacgaatttc atcgccaatc
      961 tcaattcttg caatgggtcg tccgtcacaa taaagctgaa ttccgggttc tgcatcatta
     1021 gcattggtat gatgaagcag tgctgaacca aaactccaaa ttcgtccctt gctatctcta
     1081 ccggtaatac cttttccttt ttgttcattc acttctgaaa agatcatcgg tgcagaatct
     1141 accagttctt ttaccagact tttcgcaata gggtgagagg aatgctgttc cagaccggca
     1201 attgcagatt tgatctcttc ctcggtacaa tctccgatcc ggtgaattgc tttgatgctg
     1261 aatttcccgg tggtaagtgt tccggtttta tcaaagacaa cagttttaac tttagccaat
     1321 tgttcaatgg tatcagctcc tttgaataat ataccggctt tcgctgctct tccaattgct
     1381 gcagctactg cagttggagt ggccaatccc attgcgcagg gacatgaaat aacgagtact
     1441 gcaattgcat tcattagtgc atgtgtagca gatagggaaa ttgcaaaata tgcaatcagg
     1501 aatgtcaata cggaaattgc caccacaaca ggaacaaaaa tactgctgat tttatcacct
     1561 agtttttgaa tggatggttt ttgaagttgt gcttctttca ccatggcaat aatttttgcc
     1621 agggtagttc cttgtccaac ccgtgttgct ttcattgtaa atgctccgtc tgccaccaat
     1681 gttcctcctg taattgcatc gccagtcctt ttacgaacag gtaaactttc tccgctgatc
     1741 atggattcat ctaccagaac ttcaccgtaa ataataattc catcaatcgg aattcgatcg
     1801 ccggaattga cgcgaagtac atcgtcgacc tgaacagaat ctgcaacgac ctcttcaatg
     1861 ctttctgtcc catttacaat tttaactcgt ttagcaattg ttttttggag tttcattaaa
     1921 tcgcgaaggg cagaagtggt ttgagtcact gctttgtgtt caacgtagtt tccaagcagc
     1981 accagggtaa ttatggta
//
LOCUS       DAOMYG010000519         1977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_22422_length_1977_cov_8.544745, whole genome shotgun sequence.
ACCESSION   DAOMYG010000519 DAOMYG010000000
VERSION     DAOMYG010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22422_length_1977_cov_8.544745"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PK610_13260"
     CDS             <1..195
                     /locus_tag="PK610_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008991463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRJ36957.1"
                     /translation="TSALTDSFQDDLLAPPVYTRPEEWRGRRVPDILLGGNHRDIENW
                     RMDEALRRTRERRPDLLDEN"
     gene            complement(212..574)
                     /locus_tag="PK610_13265"
     CDS             complement(212..574)
                     /locus_tag="PK610_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36958.1"
                     /translation="MFEYFFTRAFKILFAVLFGLLFGGLIVMWLWNALMPAVFDLPEL
                     TFIQSAGILLLSKILFSDPSFGFDRKCGGCGGKSRHQHWKSAMRAKMAGMPEAEREAL
                     RKKLSRCGWNQESDEKSE"
     gene            complement(586..1173)
                     /locus_tag="PK610_13270"
     CDS             complement(586..1173)
                     /locus_tag="PK610_13270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ36959.1"
                     /translation="MSHVRTAIVNVSMTESQNQEIDSTVKRERKKLFRFIRDRVRTDE
                     EAEDILQDVFYQLVESYRLMRPIEQMSSWLFTVARNKITDRYRKKQTGSLEDIAVHRD
                     DEGEPLFLSDLLRGTAEDGESMMTNELIMDTIMDALEELPSEQREVFVMHELEDKSFR
                     EIAEITGAGQNTLLSRKRYAVLFLRERLQSLYDEL"
     gene            1329..>1977
                     /locus_tag="PK610_13275"
     CDS             1329..>1977
                     /locus_tag="PK610_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36960.1"
                     /translation="MNGTDNPFSISGYEGPERFCDREEETARITNAIRNKRNLVILAH
                     RRMGKTVLIEHAFHQVKNVKGLKTFSVDLLPCENLNDFCLVMAREVVALLESKPEKLI
                     KTIRGMFRTLNPIIRYDAVTGDPAVEFQLGDKSSPQLTLTEIFRFLESLKIRMVIAFD
                     EFQQIAFFPEKNTQSLLRAEIQKCKYLSFIFSGSRQHLLGAMFAQHNKPFYQSAEI"
BASE COUNT          566 a          488 c          409 g          514 t
ORIGIN      
        1 acctctgccc ttaccgattc gtttcaggac gaccttttgg ccccacctgt atatacccga
       61 ccggaagaat ggcgaggcag gcgtgtgccc gacatccttc ttggcggcaa tcaccgcgat
      121 attgaaaact ggcgcatgga cgaagcactt cgcagaaccc gtgagcgcag accggatttg
      181 ctggatgaga attaaccttt cgaattaaaa tctattcact cttctcatcg ctttcctgat
      241 tccatccgca acgcgacaat ttcttgcgca atgcttcacg ttcagcctcg ggcatacccg
      301 ccattttagc acgcatggcc gatttccaat gctggtgacg cgatttccca ccgcatccgc
      361 cgcatttgcg atcaaaaccg aatgaaggat cactgaaaag aattttactg agtagcagga
      421 ttcctgccga ctgtataaaa gtcagttccg gtaagtcaaa tacggcaggc atcaatgcat
      481 tccacagcca catgacgatc aatccgccaa acaacagtcc gaacaatact gcaaacagta
      541 ttttaaaggc tcgtgtaaaa aagtattcaa acatatcaac taagtttata gttcatcata
      601 aagtgattgg agtcgctcgc gtaaaaaaag cacagcatat cgtttgcgcg acaacaaggt
      661 attttgtcct gctccggtta tttcggcaat ttccctgaag cttttgtcct cgagttcatg
      721 catcacaaaa acctcacgtt gttcagaagg caactcctcc agtgcatcca taatggtatc
      781 catgatcagc tcattggtca tcatcgattc accatcttca gcagttcccc gtaacagatc
      841 actcagaaac agcggttcac cctcatcatc acggtgcact gcaatatctt caagcgaacc
      901 ggtttgcttt ttccggtaac gatcggtgat cttgttgcgt gcaaccgtaa acagccagga
      961 actcatctgc tcaatcggac gcatcaaacg ataggattcc accagctggt aaaagacatc
     1021 ctgcagaata tcttcggctt cctcgtctgt acgcacacga tcgcggatga aacgaaacag
     1081 cttcttccgc tcacgcttta ccgtagaatc aatctcctgg ttctgggatt cggtcatgct
     1141 aacattaact atggcagttc ttacgtgact catctgtttc ggaagacgag cgcagaaaaa
     1201 gaatattgca agatggcgaa tttaaaacca taaacaccta aaaatcagta aaataatttt
     1261 aactcaagta aggagttgaa tccattcaga aaaaattacg taatttgcgt aatgtaaatt
     1321 acgtaattat gaacggtact gataatccat tcagcatttc gggatatgaa ggtccggaac
     1381 gtttttgcga ccgtgaagaa gaaactgcac gcattaccaa tgcgatcagg aacaagcgaa
     1441 atctggtgat actcgcccat cgccgtatgg gaaaaactgt tttgattgaa catgcttttc
     1501 atcaggtgaa gaatgtaaag ggactgaaaa catttagtgt ggacctgttg ccctgcgaaa
     1561 acctgaatga cttttgtttg gtaatggcaa gagaagtggt ggcgcttctt gaaagcaaac
     1621 ctgaaaaact catcaaaacc attcggggaa tgtttcgcac tctcaacccg attatccgct
     1681 acgatgctgt aaccggcgat ccggctgtgg aatttcagct aggtgataaa tcgtcgccgc
     1741 aacttacact gacagaaatc ttccgctttt tggaatcgct caaaatccgg atggtcatcg
     1801 ccttcgatga gtttcagcag atcgctttct ttcctgaaaa aaacacacaa tcgcttctgc
     1861 gggccgaaat tcagaaatgc aaataccttt cgtttatttt ttcgggtagt cgccagcatt
     1921 tactcggtgc catgtttgcg caacacaaca aaccctttta ccagagtgcg gaaatca
//
LOCUS       DAOMYG010000520         1973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_22489_length_1973_cov_9.248697, whole genome shotgun sequence.
ACCESSION   DAOMYG010000520 DAOMYG010000000
VERSION     DAOMYG010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22489_length_1973_cov_9.248697"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1203
                     /locus_tag="PK610_13280"
     CDS             136..1203
                     /locus_tag="PK610_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HRJ36961.1"
                     /translation="MQSPTKDELIASFDPDGVGQNNGNLFGLPFSYEHSRVAILPVPW
                     DVTVSYNEGSANGPAAILEASPQLDFYDPDDSEAWKIGFHLLPISREWRQLSEQFRVK
                     SVEYISFLENGGVVENDSAMLEVRNEVNEACKVLHQRVKERTLELMKEGKMVALLGGD
                     HSTPLGLIQALAEKHSDFGILHIDAHLDLRRAYEGFTYSHASIMYNVLQLPQVERLVS
                     VGIRDYCRQEAELANDSEGRVRVYYDADMKRDLYEGGTWQTICEDIVAHLPQEVYVSV
                     DIDGLDPKLCPNTGTPVPGGLEFEQAVYLLRTLVSSGRKIIGFDLNEVSPGDNDWDAN
                     VGARMLWKLCVFLSQSNNLKP"
     gene            1200..>1973
                     /locus_tag="PK610_13285"
     CDS             1200..>1973
                     /locus_tag="PK610_13285"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRJ36962.1"
                     /translation="MIYARLITVVFLFCSSFAVAQRVDLRKIDHYTENARKEWDCPGL
                     AIGIIHNDSVVFAKGYGTISSDSEKPVDANTLFGIASNTKAFTAAAIATFVDDKKIEW
                     DEKVVQLLPYFRLYNDYVTNEITIRDILSHRTGLRTFSGDLIWYGSEHKPREIIERAR
                     YLKPAYGFRAHYGYSNIMYLTAGEIVASLHGKSYENFITEKFFKPLGMDRSNVSIRHQ
                     KKDENVAQPHAKVDGKWIPVPYVNWDNIAPAGAINSTVND"
BASE COUNT          552 a          357 c          478 g          586 t
ORIGIN      
        1 gtggttacct gtcttcactg tattgttgat cccttaagta tggaagtaaa gaaattcccc
       61 ttatgccgat atttacttgt ttaaagaatg ctttcagtcg gggcttttct tattttcgct
      121 gactaagact ttgctatgca atcccctaca aaagatgaat tgattgcttc attcgatccg
      181 gatggagttg gacaaaacaa cggtaatctt tttggattac ctttttccta tgagcattca
      241 cgtgttgcaa ttctccctgt tccatgggat gtaactgttt cttacaacga aggctcggct
      301 aatggtccgg ctgcaatttt agaagcttct ccacaactcg atttttatga tccggatgat
      361 tcggaggcct ggaagattgg gtttcatctt ttaccaatca gcagagaatg gaggcaatta
      421 agtgagcagt tccgggtaaa atctgttgaa tacatttctt ttttggaaaa tggcggagtg
      481 gtagaaaatg attctgctat gttggaggtg cggaatgaag tcaatgaagc gtgtaaggtt
      541 ttgcatcagc gagtaaaaga aagaacgctt gaattaatga aagaaggtaa aatggttgcg
      601 ttgcttggtg gtgatcatag tacgcctctc ggattgattc aggcattagc ggaaaagcat
      661 tcagatttcg gtattctgca tattgatgca catctcgatt tacgaagagc atacgaggga
      721 tttacctatt ctcatgcttc aattatgtac aatgtgcttc agttgccgca ggttgaacga
      781 ttggtttcag tgggaattcg tgattattgc aggcaggaag cagagctggc aaatgattct
      841 gaaggaagag taagggtgta ctacgatgca gatatgaaaa gagatcttta cgaaggtggt
      901 acatggcaga ccatttgcga agacatcgtt gcgcatttgc cacaggaagt ttatgtgagt
      961 gtggatatcg acggacttga tccaaaactt tgtcccaata caggaacccc ggttccgggc
     1021 ggactcgaat ttgaacaggc tgtgtatctg ttacgtacat tggtaagcag tggccgaaaa
     1081 ataataggat ttgatctcaa tgaggtttcg cccggtgata acgactggga tgcaaacgtt
     1141 ggggcacgga tgttgtggaa gctatgtgtg tttctttcac aatctaataa tctgaagcca
     1201 tgatttatgc tcgtctgatc acagtagtat ttcttttttg ttcttcattc gcagtagcgc
     1261 agcgggttga tcttcgcaag atcgatcatt atacagaaaa tgccagaaag gaatgggatt
     1321 gtccgggtct tgccattgga attattcaca atgattctgt agtttttgca aaaggatatg
     1381 gtaccatcag ttcggatagc gagaaacccg ttgatgccaa taccttgttt ggaattgcat
     1441 ccaacaccaa ggcatttact gcagctgcaa ttgcaacatt tgttgatgac aaaaaaattg
     1501 aatgggatga aaaagttgtg cagttattgc cttatttcag attgtacaac gattatgtga
     1561 ccaacgaaat aacgattcgc gatattttat cacacagaac aggtttgcgc acttttagcg
     1621 gggatctgat ctggtacgga agtgaacata aaccccggga gatcattgaa cgtgcgcgct
     1681 acttaaagcc tgcatacgga tttcgtgccc actatggata cagcaatata atgtacctca
     1741 ctgcaggaga aattgtcgca tcgctgcatg ggaagtccta cgaaaatttc attacagaga
     1801 aattttttaa acctcttggg atggatcgca gtaatgtttc gatccggcat cagaaaaaag
     1861 atgagaatgt tgcacagcct catgcaaaag tggatggaaa gtggattcct gttccttatg
     1921 taaactggga taatattgct ccggcgggtg ccataaactc taccgtgaac gat
//
LOCUS       DAOMYG010000521         1966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_22601_length_1966_cov_9.868132, whole genome shotgun sequence.
ACCESSION   DAOMYG010000521 DAOMYG010000000
VERSION     DAOMYG010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22601_length_1966_cov_9.868132"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1011
                     /locus_tag="PK610_13290"
     CDS             73..1011
                     /locus_tag="PK610_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36963.1"
                     /translation="MKKLIRLFSAFALVISTGNVQSQAVDQFTALQSIGEGSVFQSYW
                     GLQFEGGKTSGQLDKTKRELKFELDVPERGNAFKMVDQVTKKEEINSGFGWLEGNHLT
                     KPSLYSGNQHAFAYINGLMYYLKGIKDPQNITSFQIERIYVLMTPIKGDHLSGKNAVK
                     EMKTRDHEAVLKKYFSDMKVIQQQATENFTEEEKNKLLMIEQAKKDKKAGNQAKNAAY
                     WNSEEGQRKLSEMRQPDVILVNDTPAPLYLCHGSGAYTELKPGEKKSFSCLGGKVYRG
                     TLRANNSSQYDATSNLLLDLDGKSCGRVVNASTVVR"
     gene            complement(1103..>1966)
                     /locus_tag="PK610_13295"
     CDS             complement(1103..>1966)
                     /locus_tag="PK610_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRJ36964.1"
                     /translation="VCWGLEKFGTEAQKQKYLVPLAKGEIIGAFCLSEPEAGSDATSQ
                     RTSAIEKDDHYLLNGTKNWITNGGSASVYLVIAQTYPDKGHHGINAFIIEKGMPGFTV
                     GAKEDKMGIRASDTHTLIFNDVKVPKENRIGEDGFGFKFAMKTLSGGRIGIASQALGI
                     AAGAYELALAYSKERKAFGKPIHEHQAIAFKLADMATEIDAARLLVMKAAHEKDNGQN
                     YDLSGAMAKLFASRVAMWVTTEAVQVHGGYGYVKEYHVERLMRDAKITQIYEGTSEVQ
                     KIVISRSVLKD"
BASE COUNT          585 a          435 c          401 g          545 t
ORIGIN      
        1 cagttcctga attccatttc ctgaaaaatg atggttacac tatgtttggt ttaaatctta
       61 atgctaaacc aaatgaaaaa actcattcgc ttattttccg cttttgctct ggttatatct
      121 accggaaatg ttcaatcgca ggctgtagat cagttcaccg ctctgcaaag tattggtgaa
      181 ggttccgtct ttcaatcgta ttggggactc cagttcgaag gtggcaaaac atcaggtcaa
      241 ttggacaaaa ccaaacgcga actaaaattc gagctggatg taccggagag aggaaatgct
      301 ttcaagatgg tagaccaggt caccaaaaaa gaagaaatca acagcggatt cggttggctt
      361 gaaggcaacc atttaaccaa gccatcattg tattccggta atcagcatgc atttgcctac
      421 atcaacggct taatgtatta cctgaaagga attaaagacc ctcaaaatat tacgtccttt
      481 cagattgaac gtatttatgt actgatgact ccaattaaag gtgatcacct cagcggaaaa
      541 aatgcagtga aagaaatgaa aactagggat catgaagctg ttttgaaaaa atatttcagc
      601 gacatgaaag tgatacagca acaagcaact gaaaacttca ctgaagaaga aaaaaataaa
      661 ctgttaatga tcgaacaggc gaaaaaagat aagaaagcag ggaatcaagc gaaaaatgca
      721 gcctactgga attcagaaga aggtcagcgt aaactgtctg agatgcgcca acccgatgtg
      781 attcttgtaa acgacacccc tgcaccactc tatctttgcc acggttcagg agcttatacc
      841 gaattgaaac ctggtgagaa aaaatcgttt tcctgccttg gtgggaaagt gtaccggggt
      901 acacttcgtg cgaacaacag ttcacaatac gatgcaacca gcaacttgtt gctcgatctc
      961 gacggaaaaa gttgtggtcg cgtagtaaat gcttctactg ttgtgcgcta aacctattct
     1021 ttcaataaaa aagggttgtc gtaattgaca accctttttt ttatgttttt cgaaaggcag
     1081 gtgaaacgtt ttcctctcgc tattagtctt tcagtacact acgtgagata acgattttct
     1141 gcacctcgga agtgccttca taaatctgag tgattttcgc atcacgcatg agtcgctcta
     1201 cgtggtattc cttcacataa ccataccctc cgtgaacctg aacggcttcg gtggttaccc
     1261 acattgcaac tcgtgaagca aacaattttg ccatagcacc ggagagatcg taattctgac
     1321 cattatcctt ttcatgcgca gctttcatta ccaaaagtcg tgcagcatca atttcagttg
     1381 ccatatccgc caacttgaat gcgatggcct ggtgttcgtg gatgggttta ccaaatgctt
     1441 tgcgttcttt ggaataagcc aaagccagtt cgtaagctcc tgcagcaata cctaaagcct
     1501 gagatgcaat tccgattctt ccgccactaa gtgttttcat cgcgaactta aatccgaatc
     1561 catcttcgcc aatgcgattt tccttaggga ctttcacatc attaaaaatc aaagtatgtg
     1621 tatccgaagc acgtataccc attttatctt ctttagcacc caccgtaaac ccgggcatgc
     1681 ctttttcgat aatgaatgca ttgatgccgt ggtgaccttt atcaggataa gtttgcgcaa
     1741 ttaccaaata aacagaggct gaacctccgt tggttatcca gttcttggta ccgttgagca
     1801 ggtaatgatc gtctttttca atagcggaag tacgctgtga tgtagcatcc gatcccgctt
     1861 ccggttcaga caaacagaat gcaccaatga tctcgccttt agcaagggga acaaggtatt
     1921 tttgcttttg agcttcagta ccgaatttct ccaatcccca gcatac
//
LOCUS       DAOMYG010000522         1949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_22908_length_1949_cov_9.100845, whole genome shotgun sequence.
ACCESSION   DAOMYG010000522 DAOMYG010000000
VERSION     DAOMYG010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22908_length_1949_cov_9.100845"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1823
                     /locus_tag="PK610_13300"
     CDS             <1..1823
                     /locus_tag="PK610_13300"
                     /inference="COORDINATES: protein motif:HMM:NF009397.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HRJ36965.1"
                     /translation="KANEKGKIKVKKIEDNTAEKVEIVIHLHPEASPDKTIDALYAFT
                     DCEISIAPNACVIENDKPRFLGVSEILRISADNTVELHKLELNIRLSELESMWHFASL
                     EKIFIENKIYRKIENEETWDGVIKAIDKGLKPFTANLKRGVTQDDIVKLTEIKIKRIS
                     KFDSDKADHYIESLEEEIQMVRHNLKHVVNYTIDFYKNIRKKYGTGKERKTEPRIFDT
                     IEAVKVVATNTKLYANRSEGFIGTSLKKDEFICDCSDIDDIIVFREDGRMIVSRISDK
                     KFVGKGIIHIAVWKKGDKNRIYHLVYRDGKQGAAFAKRFAVTAITRDKEYILTRGNPE
                     SKVLYFTEHPNGEAEIITVHLRARPNLKRLTFDYEMAGLAVKNMQSQGNLLTKYSVNK
                     ISQKEIGGSTLQAQQIWFDDITMRLNISGRGELLGRFRNDDKILTATQSGYYRISGHS
                     LDLHFDDDLIMIEKLNMKRPVTIVYYDGDKEAYFVKRFMLDEGDRKNHVISISEGSRI
                     ELITTQWVPRIKVEYDGRSTSKKPENINLSKLVEVRNEKAVGNKLTEFKIKQITLLDP
                     KKDDIPEDERENEAQLLADRTDNLEFDSKGGGDGEQMGLF"
BASE COUNT          633 a          370 c          452 g          494 t
ORIGIN      
        1 tcaaggcgaa tgaaaaagga aaaattaaag tcaagaagat tgaagacaac accgctgaga
       61 aggtagagat tgtcattcat cttcatcccg aagcttctcc cgacaaaacg atcgatgcat
      121 tatatgcctt taccgattgc gaaatttcta ttgcaccgaa tgcatgtgtc attgaaaacg
      181 acaaaccacg cttccttggt gtaagtgaaa ttctccggat ttctgctgat aacacggttg
      241 agttgcataa actcgaattg aacattcgtt tgagtgaact cgaatcgatg tggcattttg
      301 cttcgctgga gaaaatcttt atcgaaaaca agatctaccg taaaattgaa aacgaagaaa
      361 cctgggatgg tgtaattaag gcgatcgaca aaggtctgaa gccatttaca gccaatctta
      421 agcgaggcgt tactcaggac gatattgtta aactgactga aatcaagatc aagcggatct
      481 cgaagttcga cagcgataag gcggatcact atattgaatc gctcgaagaa gaaattcaga
      541 tggtacgcca caacctcaag catgttgtga actacaccat tgatttctac aagaacattc
      601 gaaaaaaata cggtacaggt aaagagcgta aaacagaacc aagaattttt gatacgattg
      661 aagcggtaaa agtagttgcc accaatacaa aactttacgc taatcgatct gaaggtttca
      721 ttgggacttc actgaagaag gatgaattta tatgcgattg ttccgatatc gacgatatca
      781 ttgtattccg ggaggatggt cgtatgatcg tttccaggat cagtgataag aagtttgtcg
      841 gtaagggaat cattcacatt gctgtttgga aaaaagggga taagaacagg atttaccacc
      901 tggtgtaccg cgacggaaaa cagggtgctg cattcgcaaa acgttttgca gtaacggcca
      961 ttacccgtga taaggaatac atccttacca gaggaaatcc ggaatcaaaa gttctgtatt
     1021 tcactgagca tcccaatgga gaagccgaaa tcattaccgt acacctgcgt gcgcgtccta
     1081 atttgaagcg actaacgttc gattatgaaa tggccggact tgcggtgaag aatatgcaga
     1141 gtcagggaaa tcttcttacc aagtattcag tgaataagat cagtcaaaaa gaaattggcg
     1201 gatctacatt gcaggcacag cagatctggt ttgatgatat caccatgcga ctgaatattt
     1261 cgggaagagg agaattgctg ggcagattcc gtaatgacga taagattctt acggctactc
     1321 agagtggtta ttatcgtatt agcggacatt cacttgattt gcattttgat gatgatctga
     1381 tcatgatcga aaagctgaat atgaagcgtc ctgttaccat tgtgtattac gatggcgata
     1441 aggaagccta ttttgtaaaa cggtttatgc tggatgaagg cgatcgcaaa aaccatgtca
     1501 tctccatcag cgaaggctca cgtattgaac tgatcacaac ccaatgggtt cccagaatca
     1561 aggtggaata cgatggtcgc agcacaagca agaagccgga gaatatcaat ctctctaaac
     1621 tggttgaggt tcgcaatgaa aaggctgtcg gaaacaaact gaccgaattc aagatcaaac
     1681 aaattacatt gcttgacccg aagaaggacg atataccgga agatgaacgt gagaatgagg
     1741 cgcagctgtt ggctgatcgt accgacaatc tggaatttga ctccaaaggc ggtggcgatg
     1801 gggagcagat gggattgttc tgatccttac cggagaaaat taaaaaagcc gatgtgaaat
     1861 aattcgcatc ggctttttta tgatcgtaaa ttttgttcgc ggatcaatta aagatttcaa
     1921 attcctcacc atcgaaactg gcaaacacc
//
LOCUS       DAOMYG010000523         1932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_23233_length_1932_cov_10.693660, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000523 DAOMYG010000000
VERSION     DAOMYG010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23233_length_1932_cov_10.693660"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="PK610_13305"
     CDS             <1..1275
                     /locus_tag="PK610_13305"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRJ36966.1"
                     /translation="DFVLKNTTGKKIFILRVDADRDVSTLLSGKTLLPDSSITIRVQY
                     NPKETGRFSKDIGIYVSNLNDPLTISISGEVRQLPQMSDLACPDFNRAPDASRIPDFE
                     LQVLVTDKTTGEPIAGAGVKIIYKGLPRYLLKTDKKGTAQKKVEIGYYYFVGSAEGYK
                     SAEFDAYINIRNNKVHIELEPYFQTKVDEWPNDSLFYKDRPDPILDPGKPKFEPLLTT
                     EDQLLSPPDTGRIILTPVPVKETFKVNHLVFLVDVSGSMMADGKMDLLKASMIELCNT
                     LRPEDRVTLIVYSSTARVVMENVPGNEKNLIIDKIQNMEGTGYTAGSEAMKKAYEVAQ
                     KNFIQGGNNMVIMATDGAFNLYTSDVMPTVKKYRKRGISTSIVAIKNTDRDAKSMQTI
                     ALEGGGRYIPITNMEQARAGLTEEVRTASRAE"
     gene            1408..1644
                     /locus_tag="PK610_13310"
     CDS             1408..1644
                     /locus_tag="PK610_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRJ36967.1"
                     /translation="MSDIKSRVIAIIVDKLGVDEAEVAESASFTNDLGADSLDTVELI
                     MEFEKEFNIAIPDDQAEKIATVGDAITYIQNNAK"
     gene            1667..>1932
                     /locus_tag="PK610_13315"
     CDS             1667..>1932
                     /locus_tag="PK610_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRJ36968.1"
                     /translation="MQLKRVVVTGIGALTPIGSTAPEYFDGLVKGVSGAALITRFDAS
                     KFKTRFACEVKNFNPEDHFERKEARKIDQFTQFALVAVAEAMKDS"
BASE COUNT          617 a          379 c          449 g          487 t
ORIGIN      
        1 gattttgttt taaagaatac caccggaaaa aaaatattta ttctgcgtgt ggacgccgat
       61 cgtgatgtga gtaccttgtt gtcgggaaaa acgcttttac ctgacagttc aattaccatt
      121 cgggtgcaat acaatcccaa agaaacaggc aggtttagca aggacattgg tatttatgta
      181 agcaatctaa atgatccgct taccatcagt atttctgggg aagttcgcca attaccacaa
      241 atgagcgatc ttgcctgtcc agattttaac cgagctcctg acgcaagcag aattcccgat
      301 tttgaactgc aggtgttggt taccgataaa accaccggag aaccaatagc cggagcaggt
      361 gtaaaaatta tctataaagg attgccccgg tatcttttaa aaacagataa aaaaggcaca
      421 gcgcaaaaaa aggtggagat agggtattac tattttgtag gaagcgccga aggatacaaa
      481 agcgcagaat ttgatgcata tataaatatc cggaacaaca aagtacatat tgagttggag
      541 ccttattttc aaactaaggt tgacgaatgg ccaaacgatt cactgttcta taaagacaga
      601 cccgacccca ttttggatcc gggaaagcca aagtttgaac cgttactaac tactgaagat
      661 caattgttaa gtccacccga caccggcaga attattttga ctccagttcc ggttaaagag
      721 acgtttaaag taaaccattt ggtttttttg gtggacgtga gcggatccat gatggcagac
      781 gggaaaatgg atctactaaa agcatcaatg atagaattat gcaacacgtt gcgccctgaa
      841 gaccgggtta cgcttatcgt ttattcctca acagcccggg tggttatgga gaatgttccg
      901 gggaatgaaa agaaccttat cattgacaaa atccaaaaca tggaaggcac cggttacact
      961 gccgggtcgg aagccatgaa aaaagcatat gaagttgctc aaaagaactt tattcaggga
     1021 ggaaacaaca tggtcattat ggcaacggat ggcgcgttta acctttatac tagcgatgta
     1081 atgccaacag taaagaaata ccggaaaagg ggaatatcga catctattgt ggcaatcaag
     1141 aataccgacc gcgatgcaaa gtcgatgcaa accattgctt tggaaggtgg cggacgatat
     1201 attcccatca caaatatgga gcaggcaaga gcaggcttga ccgaagaagt tcgcaccgct
     1261 tcaagggcag aataaggagt tattagcgtt ttatcgttgt aaaccgctca ttattaaatg
     1321 cttcatttgt ctgaaaaaag attttgatat gtttagtaaa ggttttttct ttgcacccag
     1381 taataccaaa taagaaagga gaaaaacatg tctgacatca agtcaagagt aatcgcgatc
     1441 atcgttgata aactgggggt tgacgaagct gaagttgctg aatctgcaag cttcacaaat
     1501 gacctcggtg ctgactcact ggacacagtt gagctcatca tggaatttga aaaggaattc
     1561 aacattgcga ttcccgacga tcaggctgag aagatcgcta ctgtaggtga tgctattacc
     1621 tacattcaga acaacgcgaa gtaatcttcc ttcaaaaaca ggtcctatgc aattgaaacg
     1681 agttgtcgta acagggatag gtgcgcttac accaataggt agtactgcgc ccgaatattt
     1741 cgacggcctg gtaaagggtg tcagcggtgc tgcgcttatt acccgttttg atgcgagcaa
     1801 attcaagaca cgctttgcct gcgaggtcaa gaatttcaat cccgaggatc actttgaaag
     1861 aaaagaagcg cgtaagatcg atcagtttac gcaatttgct ttggtcgcag tggccgaagc
     1921 gatgaaggat tc
//
LOCUS       DAOMYG010000524         1929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_23279_length_1929_cov_11.785486, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000524 DAOMYG010000000
VERSION     DAOMYG010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23279_length_1929_cov_11.785486"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="PK610_13320"
     CDS             complement(<1..1217)
                     /locus_tag="PK610_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRJ36969.1"
                     /translation="MSVPVLLLTPPFTQLNTPYPATAYLKGFLATRNIQAEQADLGID
                     VTLALFSKSGLKDVFRLAEAANPKDENCKRMLALKEEYLQTIDAVIRFLQDKDPTLAH
                     SICSRNYLPESARFLQTENLDWAFGEMGTRDRARHLASLYLEDLNDLIVSCIDPEFGF
                     TRYAERLGRCANTFDALHDHLNAAPGFIDTITLKILDHYLKENNPALVAFTVPFPGNL
                     YSAFRCAQHIRNHYPHMKVAMGGGFANTELRSVSDPRVFDYFHYITLDDGEAPLECLM
                     ELIDGKRNENELKRTFLLRNHQVEYIHHTSIPDYKQAQTGTPDYSGLRLKEYLSVIEI
                     ANPMHRLWSDGRWNKLTLAHGCYWGKCTFCDVSLDYIKIYEFAAAKIIVDRMETLIAQ
                     TGESGFHFVDEAAP"
     gene            complement(1221..>1929)
                     /locus_tag="PK610_13325"
     CDS             complement(1221..>1929)
                     /locus_tag="PK610_13325"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRJ36970.1"
                     /translation="YTIVYAIWDEEEQGKIGSAAYANMARNDGDSIRGVINMDAIAWD
                     GNGDNAAMIHTSSISNSLNIAAMIDNVNNTYNIGLDLYLTNPGATYSDHASFWNKQYG
                     AVLVIEDWAFDANPHYHTVTDKVQYFNIPYFEKMAKLSIASTAELAVPISFLGSEQEE
                     TSTPVQVFPNPFSDGIWIRSERALSFQLCDFQGKILHLGSTNNQTYIALSDLPTGIYV
                     LHIQDNKGVSTVKVIKY"
BASE COUNT          506 a          422 c          452 g          549 t
ORIGIN      
        1 ggagcggctt catccacaaa atgaaatcca ctttctccgg tttgagcgat gagtgtctcc
       61 attcgatcca caatgatttt cgcagcggcg aattcgtaaa tctttatgta atcaagcgag
      121 acatcacaaa aggtgcattt tccccaataa caaccatgtg caagtgtaag tttgttccat
      181 ctcccatcgc tccacagcct gtgcatggga ttggcaattt ctattaccga caaatattct
      241 tttagccgta accccgaata atccggcgtg cctgtttgag cctgtttgta atcgggaatg
      301 gaagtgtgat ggatgtattc tacttggtga ttgcgaagca gaaatgtgcg tttaagttcg
      361 ttttcgtttc gttttccgtc aatcaattcc attaaacatt ccaatggagc ttctccatca
      421 tcaagcgtga tgtaatggaa ataatcaaac acacgaggat cgctgacaga tcgcaattcc
      481 gtgttcgcga atccgcctcc cattgctact ttcatatggg gataatggtt gcgaatgtgt
      541 tgtgcgcagc gaaaagcgct gtataaatta ccgggaaacg gaacagtaaa tgcaaccagt
      601 gcaggattgt tttccttcag gtaatggtcg agaattttca gtgtaatcgt atcaatgaaa
      661 ccgggagcag cattcaggtg gtcatgcaat gcatcgaatg tattcgcaca tctccccagg
      721 cgttctgcgt agcgggtaaa tccaaattca ggatcgatgc aggaaacgat cagatcattc
      781 agatcttcga gatacagcga agcgagatgg cgtgcacgat cgcgtgttcc catttctccg
      841 aaggcccaat ctaaattttc tgtctgcaga aaacgtgcgg actccggcaa ataatttctg
      901 ctgcaaatgg aatgtgcaag ggttggatct ttatcctgca aaaaccgaat cacagcatct
      961 atggtttgta aatactcttc tttgagtgcg agcattcgtt tgcagttttc gtccttagga
     1021 ttggctgctt ctgcgagacg gaaaacatcc ttcaatccgc ttttcgaaaa gagcgcaagg
     1081 gtaacatcta ttcccaggtc ggcctgttct gcctgtatgt ttctggtggc cagaaatccc
     1141 tttaaatagg cagtagccgg atagggggtg ttgagctgtg taaatggcgg agtaagaagt
     1201 aaaaccggga cagacatcaa tcagtatttg atcactttaa cagtactcac gcctttgttg
     1261 tcctgaatat gaagaacata tatccctgta ggtaaatcgc taagtgcgat gtaggtttga
     1321 ttgtttgtgc ttcccaaatg aaggatttta ccttgaaaat cgcacaattg aaagctgagc
     1381 gcacgctcgc ttcggatcca gattccgtcg gaaaaggggt tcggaaatac ctgaacgggc
     1441 gtgcttgttt cttcttgttc cgacccaaga aaactgatgg gcactgccag ctctgcggtg
     1501 gaagcaatgg agagttttgc cattttctcg aaatagggaa tattaaaata ctgcacttta
     1561 tcggtaacag tatggtagtg cggattggca tcaaaagccc agtcctcaat aaccagcacc
     1621 gctccgtact gtttgttcca gaaagaagcg tgatcgctgt acgttgcgcc gggattggta
     1681 aggtacagat cgagtccgat gttgtacgtg ttattcacgt tatcgatcat cgcagcgata
     1741 ttgagtgaat tgctgatgga agaagtatga atcattgcgg cattgtcgcc gtttccatcc
     1801 catgcaattg catccatgtt tatcaccccg cgaatggaat caccatcgtt tctggccata
     1861 ttggcatagg cggcacttcc aatttttcct tgttcttctt catcccaaat agcataaaca
     1921 atggtgtat
//
LOCUS       DAOMYG010000525         1927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_23330_length_1927_cov_6.365919, whole genome shotgun sequence.
ACCESSION   DAOMYG010000525 DAOMYG010000000
VERSION     DAOMYG010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23330_length_1927_cov_6.365919"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PK610_13330"
     CDS             <1..741
                     /locus_tag="PK610_13330"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRJ36971.1"
                     /translation="TLFTGRQLIFREHVDSTNNYAATLMENSKMAEGAVVMAHEQSAG
                     RGQRGTEWTAEKGKNLTVSIVYYPTFLNASDRFLLSQCVALALREVVEELAGRSVIKW
                     PNDILCDEKKVAGILIETSFSGEKMQHAIIGIGLNVNQTGFPDGLNAASLVEFSGYEL
                     ELKSVLALVCERLEKWYLRLRSGKKDEIRKEYLEHLFRFASESRFSTAQGAFNGIITG
                     IASDGKLQVLESNGKLWEFDVKEVSLVI"
     gene            complement(725..1135)
                     /locus_tag="PK610_13335"
     CDS             complement(725..1135)
                     /locus_tag="PK610_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36972.1"
                     /translation="MTRIESNTVRVKNSSQEVFVFLRDMNNFIQLLPQEKISDWKSDN
                     DSCSFRVQGAVVIPLIVVSAEAPGHIHIKSGDNAPFPFTLDINIVTLAELECEGNLIF
                     NGEMNAFLRMMAVGPLTNLFNYVANKLVQVKSLS"
     gene            complement(1132..1782)
                     /gene="pyrE"
                     /locus_tag="PK610_13340"
     CDS             complement(1132..1782)
                     /gene="pyrE"
                     /locus_tag="PK610_13340"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRJ36973.1"
                     /translation="MSVNKDSALKVAEFLLQVKAVKLQPNKPFTWASGLRSPIYCDNR
                     VTLSYPHIRTFIRQELSKVIAQEFAKGDIIVGVATGGIAQGVLVAEELGLPFAYVRPE
                     PKSHGMGNQVEGVIEPGQSVIVVEDLVSTGKSSLNAVDAITAAGGVVKGMVAIFTYGF
                     EDAAKKFKDKNVNLATLTNYDILIEQAVASNYVSEKDLGSLREWRVDPKSWSEKQA"
BASE COUNT          554 a          410 c          434 g          529 t
ORIGIN      
        1 acacttttta cagggcgcca gttgattttc cgggagcatg ttgattccac caacaattat
       61 gctgccaccc tgatggaaaa ctccaaaatg gcagaaggtg ctgttgttat ggcacatgaa
      121 caaagcgccg gaagaggtca gcgtggcacg gaatggacag ccgaaaaagg aaaaaacctt
      181 acggtttcca tagtgtatta ccctacattc cttaatgctt ccgaccgttt tctattgagt
      241 cagtgtgtgg cattggcatt gcgcgaggta gtggaggagt tggcaggacg ttctgtcata
      301 aaatggccaa atgatatttt atgcgacgaa aaaaaggtgg caggaatact gatcgaaacc
      361 tccttctcag gcgaaaaaat gcaacatgca atcatcggga tcggattaaa tgttaatcag
      421 accggttttc cggatggttt gaatgccgct tctttggttg aattttcagg atacgaactg
      481 gagctgaagt cggtattggc cttagtttgc gaacgacttg aaaagtggta tttacgtctg
      541 cgttccggaa aaaaagatga aatccggaaa gaatacctcg aacacctttt tcgttttgca
      601 agcgaatcac gcttttcaac tgctcaggga gcattcaacg gaataattac cggaattgct
      661 tccgatggga aattacaggt gctcgaaagc aatggaaagt tgtgggaatt cgatgtaaag
      721 gaagtcagct tagtgattta acctgaacaa gtttgttggc gacgtaattg aatagattgg
      781 taagtggacc aacagccatc atccgcaaaa acgcattcat ttcaccgtta aaaatcaggt
      841 ttccttcaca ttccaattcc gcaagcgtaa caatattgat atcgagtgtg aacggaaatg
      901 gagcgttgtc gcctgatttg atatgaatat gtccgggtgc ttcagctgaa actacaataa
      961 gcggtattac tactgcacct tgaacacgga aactacagga atcattatcc gatttccagt
     1021 ccgaaatttt ttcctggggc aggagctgaa tgaaattgtt catgtcgcgc agaaagacaa
     1081 agacttcctg cgagctgttt tttacacgga cggtattgga ttcgatacgg gtcatgcttg
     1141 cttttcactc catgatttag ggtcgactct ccattcgcga agcgagccaa ggtctttttc
     1201 cgatacgtaa ttggatgcaa cagcctgctc aatcagaata tcgtaattgg taagtgttgc
     1261 gaggtttaca tttttatcct tgaatttttt agcagcgtct tcgaatccgt aagtgaaaat
     1321 agccaccata ccttttacca caccaccagc agcagtaatg gcatcaacag cattgagact
     1381 gctttttccg gttgatacga gatcttccac aacgattact gactgtccgg gttcaatcac
     1441 accctcaacc tggttgccca ttccgtgcga ttttggttcc ggacgaacat aggcaaaagg
     1501 caagcccaac tcttcagcaa cgagtacccc ttgtgcaatt ccgccggtgg ctacaccaac
     1561 aatgatgtca cctttggcaa attcctgggc aattacttta cttaattcct gacgaataaa
     1621 cgttcggata tgaggataag acaacgttac ccgattatcg caataaatcg gcgaacgcaa
     1681 tcctgatgcc catgtgaagg gtttgttggg ttgtaattta acagctttca cctgcagaag
     1741 aaattctgct acctttaggg ctgaatcttt gttgacagac ataggcggca aatgtataaa
     1801 gttttcatca acaacattcc cgtgatcctg cagtcgggag gggaaaatct tccacaggga
     1861 gaatggccat tattattaac ttttccttct aataaaaaaa caaataatac cattatcgac
     1921 ctaatag
//
LOCUS       DAOMYG010000526         1905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_23685_length_1905_cov_7.627568, whole genome shotgun sequence.
ACCESSION   DAOMYG010000526 DAOMYG010000000
VERSION     DAOMYG010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23685_length_1905_cov_7.627568"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PK610_13345"
     CDS             complement(<1..374)
                     /locus_tag="PK610_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRJ36974.1"
                     /translation="MSQQVIQEGEFEYIEKGEGQALVLLHGLFGGLSNFDDVINHFSP
                     QCRVIVPQLPIYTLPILNTNISALSKFILRFLDHKKLEKVTLLGNSLGGHVGLVFTKE
                     NPDRVERLILTGSSGLHENAFGG"
     gene            648..1115
                     /gene="mraZ"
                     /locus_tag="PK610_13350"
     CDS             648..1115
                     /gene="mraZ"
                     /locus_tag="PK610_13350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRJ36975.1"
                     /translation="MAGFIGEYEVKLDDKGRFVLPAGIKKQLEPTDHDTFVVNRGFEQ
                     QLNLYTMKEWENLMNRVTKKVNQFTEEGRRLLRQFRNGAVPVSIDSSGRLLLPQNLMQ
                     YAGIKKELTVVGAFDRVELWDRSKYEQEMKEGLAEFSMLAEKLLGNLGEEDGQ"
     gene            1105..>1905
                     /gene="rsmH"
                     /locus_tag="PK610_13355"
     CDS             1105..>1905
                     /gene="rsmH"
                     /locus_tag="PK610_13355"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRJ36976.1"
                     /translation="MASNSYHIPVLLHAAVKGLNLQPDGIYVDVTFGGGGHSRLILNQ
                     LQGGRLIAFDQDPDAKANVPDDERVSFIPRNFSQMLSSLEECNAVPVNGILADLGVSS
                     HQFDDPTRGFSLRFDAPLDMRMDQNSHPDAQEVINHYDENRLKTILREYGEVEQAGRL
                     VHYITERRKQNPITTTGELLSIIDRVASPKKVKQYQAKVFQAIRIEVNDEMRVLREFL
                     EQSLKALAPGGRLVVIAYHSLEDRLVKNFMRTGNLEGEDRLPVGPDDAI"
BASE COUNT          516 a          403 c          472 g          514 t
ORIGIN      
        1 cccccaaatg cattttcgtg caaacctgaa ctgccggtaa gtatgagtcg ctcaacgcgg
       61 tcaggatttt ctttggtgaa taccaatccc acatgtccgc ccaatgaatt gcccagcagc
      121 gttacctttt ccagcttttt atgatcaaga aaacgcagga taaatttcga taatgcgctg
      181 atattcgtgt ttaggatagg aagggtgtaa atgggtaatt gagggacaat aaccctgcac
      241 tgaggtgaga aatgattaat cacgtcatcg aaattactga gccctccgaa aagtccgtga
      301 agcaggacaa gtgcctgacc ttcacctttt tcgatgtatt caaattctcc ttcttggatc
      361 acttgttgtg acatatccgc taagctttgg tgtaggctca aagttagcaa aagcctgcaa
      421 tttcagtccg tttgcgggcg gatgtatcaa cactccattg tggggctagt tttgttcata
      481 atccccactt tctcccactg gaaactttct ttgattttat tggataaatc agaagaaact
      541 gtaacttcgt taaccctatt tcgtagaaac ttatcaacat tgtgggagta agtggagtga
      601 agtgggagac tttaattatt tttacgcctt attggtggta agctgttatg gcaggattca
      661 ttggagaata tgaggttaag cttgatgaca agggaaggtt tgtccttcct gcgggtatca
      721 agaagcagct cgagcctacg gatcacgaca cgttcgtggt taaccgtggt ttcgagcagc
      781 agcttaatct gtataccatg aaggagtggg agaatctgat gaacagggtg acgaaaaaag
      841 tcaaccagtt cacagaagag gggcgacgcc tgcttagaca gttccgtaat ggggcggttc
      901 ccgtttcgat cgattcgagt ggaagattac ttctgccgca gaatctcatg cagtacgccg
      961 gaatcaaaaa agaactcacc gtggtgggag cttttgatcg ggtggagctt tgggatagat
     1021 cgaaatatga acaggagatg aaggaaggat tggcggaatt ctccatgctt gccgaaaagt
     1081 tacttggcaa tcttggagaa gaggatggcc agtaacagct accacatccc tgttctttta
     1141 catgctgctg ttaaggggct caatcttcaa cccgacggaa tttatgtgga tgtcaccttt
     1201 ggaggtggcg gtcattcccg tttaatcctg aatcaacttc agggaggccg actcatcgct
     1261 tttgaccagg atccggatgc caaggcaaat gttccggatg atgaacgtgt aagtttcatt
     1321 ccaaggaatt tttcgcaaat gctctcttca ctcgaagaat gcaatgctgt tccggttaac
     1381 ggaatccttg ctgatctcgg cgtttcatcg catcaatttg atgatccgac tcgcggtttt
     1441 tcattgcgct ttgatgctcc tctcgatatg cggatggatc agaactcaca tcccgatgcg
     1501 caagaggtga tcaatcacta cgatgaaaat cgtttgaaaa ccattttgcg cgaatacgga
     1561 gaagttgaac aagccggacg attggtgcac tacataaccg aaagaagaaa acagaatccg
     1621 attaccacca ccggagaatt gctttccatc atcgatcgtg ttgcatcgcc taaaaaagta
     1681 aaacaatacc aggcaaaagt ttttcaggca atccggattg aagtcaatga tgaaatgcgg
     1741 gtgttgcgtg agtttctgga gcaaagttta aaagcactgg ctccgggcgg acgattggtg
     1801 gtaatcgctt atcactccct cgaagatcga ctggtgaaaa attttatgcg caccggaaat
     1861 cttgaaggag aggatcgcct ccctgtcggg ccggacgatg ccata
//
LOCUS       DAOMYG010000527         1900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_23784_length_1900_cov_8.630352, whole genome shotgun sequence.
ACCESSION   DAOMYG010000527 DAOMYG010000000
VERSION     DAOMYG010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23784_length_1900_cov_8.630352"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..417)
                     /locus_tag="PK610_13360"
     CDS             complement(61..417)
                     /locus_tag="PK610_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002664569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 domain-containing protein"
                     /protein_id="HRJ36977.1"
                     /translation="MKHLLTLEEAAQFLLSILLFNQLHYAWWVFPAWLLVPDLSMIGY
                     VINTQIGALLYNFFHHKLLAIAVYALGIWLQQPQLILAGIILFGHSAMDRVFGYGLKY
                     SDDFKNTHLGWIGGKS"
     gene            complement(713..>1900)
                     /locus_tag="PK610_13365"
     CDS             complement(713..>1900)
                     /locus_tag="PK610_13365"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HRJ36978.1"
                     /translation="SITEKRFIELSYRKNFFSFEFASLDFVLPEKILYSYKMEGLDED
                     WSTPSTRNFASYTDLQGGDYVFRVKATNNDGVWNEKGVALHIRIIPPFYKTRLFYSLS
                     VFFTIVLVFIIIRMRVASINAEKKRLEEMVAQRTRELAEKNQDIMSSINYARRIQDAI
                     LPETNLIAQALPDSFILYRPKDIVSGDFYWFAELGNKKLIASVDCTGHGVPGAFMSMI
                     GHNLLNQIVLEKQVSDPAEILEILRVNVRRALKQEGRAVDSQDGMDLALAVLETDTRK
                     LSYAGAFRPIIIVRKGSLEKIEGVKSPIGMTRADLLEPYITHTRNLNLGDSFYMYSDG
                     YVDQFGGSNGKKFMGKNLHALILEISDKPMHEQKEILLARFESWKGELEQIDDVLIIG
                     VRL"
BASE COUNT          534 a          415 c          436 g          515 t
ORIGIN      
        1 tgcgcgcgac gccgggcaaa cgcgtgctcc agtagccggc ggaggcgttc atcgcctccc
       61 tcaggacttc ccccctatcc aacccaaatg cgtattctta aaatcatccg aatacttcaa
      121 tccgtaaccg aaaacccggt ccatggccga atgaccgaaa aggataattc cagccagtat
      181 cagctggggt tgctgtagcc aaattcccaa ggcgtaaact gcaatggcga ggagtttatg
      241 gtgaaagaaa ttgtatagca aagctccaat ttgggtatta ataacgtaac cgatcatgga
      301 tagatcagga acaagcaacc aggcagggaa aacccaccat gcgtaatgca gttgattgaa
      361 cagaaggata gatagaagaa attgtgctgc ttcttcgagg gtgaggaggt gtttcatgga
      421 gaagggtaaa atgtgacaga tggacaattc ggcaagctca gtgctggtga atggagggaa
      481 gtaaatttta aagaactctt ttttttgaga agaaaaataa attagaaata tttatagttt
      541 tcaattgaat gtactgaact tgtttattct tcagactcaa cattaaaaat taagatatga
      601 aaagtgctat tcacaattga accaccctca atcaaaccct taccccaatg ataagaacat
      661 catcaaccac cgacgatccg ctgcgcggat gtcggggtag tgatgttcta aatcataggc
      721 gtacgcctat gataagaaca tcatcaattt gctccaactc ccctttccaa ctttcaaaac
      781 gcgctaacaa aatttccttt tgttcgtgca tgggtttgtc gctgatttca aggatgaggg
      841 cgtgaaggtt tttgcccatg aattttttgc cgttgctgcc gccaaattgg tccacatagc
      901 cgtcggaata catgtagaaa ctgtcgccga gattgaggtt gcgtgtatgc gtgatgtagg
      961 gctcaagcag atctgcgcgg gtcatgccta tgggtgattt tacgccttct attttttcga
     1021 gtgatccctt gcgtacaata ataatcggac gaaatgctcc tgcataactg agtttacggg
     1081 tatcggtttc cagtactgcc aaagcgagat ccattccgtc ctgagagtcc actgccctgc
     1141 cctcttgctt tagggcgcga cgcacattca cgcgcagaat ctcaagaatt tcggcaggat
     1201 cgctgacttg tttttcgaga acgatctggt taaggagatt gtgtccgatc atgctcataa
     1261 atgcacccgg aacaccatgt cctgtgcaat ctacacttgc tatgagtttt ttgtttccga
     1321 gctctgcaaa ccagtaaaaa tcgccgctta caatgtcttt gggacggtac agaataaacg
     1381 aatcgggtaa tgcttgtgca atcaggtttg tctccggtaa aatggcatcc tgtatacgac
     1441 gcgcataatt gatggagctc atgatatcct ggttcttctc cgcaagttca cgtgtacgct
     1501 gtgcaaccat ttcttcgagc cgttttttct ctgcgttgat gcttgctaca cgcatgcgga
     1561 tgatgataaa gaccaacaca atggtaaaga aaacactgag cgaataaaac aaacgtgttt
     1621 tgtaaaaagg cggaatgatg cggatatgca aggccactcc tttttcgttc catacaccat
     1681 cattgttggt tgcctttaca cggaaaacgt aatcgccacc ttgcagatcg gtatagcttg
     1741 caaagttacg tgtggaaggt gtggaccaat cctcatcgag tccttccatt ttatacgaat
     1801 agagaatttt ttcgggcaac acaaaatcga gcgaggcgaa ttcgaaggag aagaaatttt
     1861 tacggtagct taattcgata aacctttttt cggtaatgga
//
LOCUS       DAOMYG010000528         1887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_24036_length_1887_cov_8.455786, whole genome shotgun sequence.
ACCESSION   DAOMYG010000528 DAOMYG010000000
VERSION     DAOMYG010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24036_length_1887_cov_8.455786"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PK610_13370"
     CDS             complement(<1..363)
                     /locus_tag="PK610_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36979.1"
                     /translation="MNRFLIAGGLIAAMALVAFRMPAKEPVDLEDAIKKGMVKIEPVS
                     KGGYSGDCINLKLTNLSKGELNLVLPAGSVFHPEDDGMQDIFVPQDQLFVLEKGKTLQ
                     RPVRGYCCQASDRSPSSGT"
     gene            578..1849
                     /gene="hemA"
                     /locus_tag="PK610_13375"
     CDS             578..1849
                     /gene="hemA"
                     /locus_tag="PK610_13375"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRJ36980.1"
                     /translation="MKHFRVIALTHKKAKLDDIGQFHLEDSQQEQRLKNLRNNARLEE
                     LMFLSTCNRVEFYFVTNENLDDDFLIEFFHAFNPGWSETQLHKAIQVAEFFEGEEAIR
                     HVFNVASSLDSMVVGEREIITQVRNAYEYCHLIGITGDTIRLVMKKTVEAAKEVYTHT
                     DIATKPVSVVSLAFRKLRDLNIHEDARILFVGAGQTNTNMARFLSKNGYHNFVVFNRS
                     MGNGLALANELKCELKELKDLAKYKAGFDVIIACTASAEPVITPEIYDELLNGDINIK
                     VVVDLAIPNDFDKKIMEKHKVKLIDVANLREEADANLKEREKELTRCNLLIDRLLKEF
                     REVYRVRQVERAMAEVPSKMKEIKDVAINTIFAKEIQGMDPSSKEVMEKVLVYLEKKY
                     ISLPMKMAKEILLDSPKTTANTESETSKELI"
BASE COUNT          622 a          374 c          382 g          509 t
ORIGIN      
        1 agttcctgaa gaaggacttc tatcggatgc ctgacaacaa tatccgcgaa caggacgctg
       61 caatgttttt cctttttcca aaacaaacaa ttgatcctgc ggtacaaaaa tatcctgcat
      121 accatcatct tcaggatgaa aaaccgatcc ggccggaaga acaagattta actctccctt
      181 tgaaagattg gtcaatttta aattgataca atcgccggaa taaccacctt ttgaaaccgg
      241 ttcaattttt accattccct ttttgatggc atcttcaaga tccacaggtt cttttgcagg
      301 cattcgaaat gccaccaatg ccattgcagc gatcagtcct cctgcaatta aaaaacgatt
      361 cattcgcata gctcctcctt ttcttactat atgtaataag cggaacaaag gttacggaaa
      421 gagctgacag aaatcagtaa tcaccataaa catgttgttt atcaataatt taaacccaaa
      481 aacatattta gaaccattcc aaagaagccc ggctgacaat tccatgacaa aacggtttag
      541 gcttttaggg aaatttgaat cacaaaattt agaggcagtg aagcatttca gggtaatcgc
      601 tttaacacac aagaaagcga aacttgacga catcgggcaa ttccacctcg aggacagtca
      661 gcaagagcag cggttgaaaa atctccggaa caatgcgcgt ttggaggagt tgatgtttct
      721 atcaacctgc aacagggtag aattttattt tgtaaccaat gaaaatcttg atgatgattt
      781 tctcattgag tttttccacg catttaatcc gggatggagt gaaacacaat tgcacaaagc
      841 cattcaggtt gcagagtttt ttgaaggaga agaagccatt cgtcatgtgt ttaatgttgc
      901 ctcctctctc gattcgatgg ttgttggaga acgtgaaatc attactcaag tgcgtaatgc
      961 ctacgaatac tgtcatttaa ttggcataac gggcgacacc atccgccttg taatgaaaaa
     1021 aaccgtggag gccgcaaaag aagtttatac gcacacagat attgcaacaa aaccagttag
     1081 tgttgtttct cttgcctttc gcaaactgcg cgatttgaat attcatgaag acgcgcgcat
     1141 tctcttcgtt ggtgcaggtc aaacaaacac caatatggcg cgttttctta gcaagaacgg
     1201 ttatcacaat ttcgttgtgt ttaatcgctc gatgggaaat ggattagcgt tggcaaacga
     1261 attaaaatgc gaactaaagg agttaaaaga tttagccaaa tacaaagcag gttttgatgt
     1321 gataattgcc tgcaccgcgt cagcagaacc ggttattact cccgaaattt acgatgagct
     1381 gcttaatgga gacatcaata taaaagttgt agttgatctt gctatcccca atgattttga
     1441 caaaaagatc atggagaaac ataaagtgaa gcttattgat gttgccaatc tcagggaaga
     1501 agcagatgct aatcttaaag agcgtgagaa agaactcacc cgttgcaatt tactgatcga
     1561 tcgtctgctc aaagaattca gagaagtgta cagagtacgt caggtggagc gcgcaatggc
     1621 tgaggttcca agcaaaatga aagagataaa ggatgtagct atcaatacta tttttgcgaa
     1681 agaaattcaa ggcatggatc catcctcgaa agaagtcatg gaaaaggtat tggtttatct
     1741 tgaaaagaaa tatatttcac tacccatgaa aatggcaaag gaaatattac tcgacagtcc
     1801 taaaaccact gcaaatacag agagtgaaac aagcaaggaa ctcatctgaa ttgaatactg
     1861 aaattgttta tctttcccct cagtaac
//
LOCUS       DAOMYG010000529         1884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_24085_length_1884_cov_9.034445, whole genome shotgun sequence.
ACCESSION   DAOMYG010000529 DAOMYG010000000
VERSION     DAOMYG010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24085_length_1884_cov_9.034445"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..265
                     /locus_tag="PK610_13380"
     CDS             95..265
                     /locus_tag="PK610_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36981.1"
                     /translation="MSRLEFARQRLIGMINRIETAEEIQELVDLLYLREEEKKREQKE
                     KETAKSTKPKKP"
     gene            262..552
                     /locus_tag="PK610_13385"
     CDS             262..552
                     /locus_tag="PK610_13385"
                     /inference="COORDINATES: protein motif:HMM:NF024232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HRJ36982.1"
                     /translation="MNSFFRIIAHLEGWSYLILLLIAMPMKYIWSNPSLIRPVGMAHG
                     LLFVLYLLLVFLLSREHKWDKKFTLFAYAAAFLPIATFIVSNRVKQHSGTKE"
     gene            complement(580..>1884)
                     /locus_tag="PK610_13390"
     CDS             complement(580..>1884)
                     /locus_tag="PK610_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36983.1"
                     /translation="DLERLQDEKYYLVHQFYHPGVDKVFALIAFKEKGKLLQQSPHSR
                     VPLLEHYQLLRIDRDLNMEVLREWNFQYKMELVRAKKLEYTDDEHFLGEERNHRFPDP
                     NASMLYVFAPAGSAFISNKDPKKGNWLIVKVSKEGAIAEMPLLLYGSGWKMMDIMHRN
                     DTLLLWGPAKPNRYYTALPTEPRHEISKNGKRYQGFQLVRIVGDRIDKISYHHQRSFH
                     QQADKQAKRLGISYRGRQFLFSYAKMGPKGEFFVGGQRIMESEVRETNGTNGQTRSHT
                     VRNGYRQPLLICFDEELKLDIVYGYRYYRWLFRRDDAVSPMWVRRSPEGKWYWEVSEL
                     YDVRTYGYTQPVFNDNVFTQVFGPSVYTSMNKYLFFPRLIPIDPSKAKMEEPVNIGLN
                     SKGRQRYYSDNLIPWKYLSDGSILYIGFDGSYSKIWLGRVRL"
BASE COUNT          564 a          422 c          348 g          550 t
ORIGIN      
        1 gttggaagct taatttttgg cgaaagaaat taaatattca ggttaataca attcattatc
       61 ccattaccgt aaaataatta ccgataccaa gaacatgtca agactcgaat ttgcccggca
      121 acgattaatt ggaatgatta accgaattga aactgcagaa gagattcagg aactcgttga
      181 tcttttgtat ttacgcgagg aagaaaaaaa gcgggaacaa aaagaaaagg aaacagcaaa
      241 atcaacaaaa ccaaaaaaac catgaattca tttttccgga taattgcaca tctcgaaggc
      301 tggtcatatc tcattttact gttgattgcc atgcccatga aatacatatg gagcaatcct
      361 tcattaatcc gtccggttgg aatggcgcat ggtttattgt ttgtgctgta ccttctgctt
      421 gtattcttgc tcagcagaga acataaatgg gacaagaaat ttactctctt cgcttatgct
      481 gccgcatttt tgcccatcgc aacattcatc gtctccaatc gtgttaaaca acattccggc
      541 acaaaagaat aatgcctgtc acaacaattt ctccggaatt tacaacctca ctcttcctaa
      601 ccagatcttg gaatagcttc cgtcgaaacc aatatagaga atcgatccgt ctgacaaata
      661 tttccaggga atcaggttat cggaataata ccgttgacgt cctttactgt ttagaccaat
      721 attcaccggc tcctccattt ttgctttcga aggatcaata ggaatcaatc gcggaaagaa
      781 caggtattta ttcatacttg tgtaaacgga tggtccaaat acctgagtaa aaacattatc
      841 attgaataca ggctgggtat atccataggt tcgcacatcg tacaattccg aaacttccca
      901 ataccattta ccctccggag aacgacgcac ccacataggg ctaacggcat catctcgacg
      961 gaataaccat ctgtaataac gatatccgta aacgatatcc agtttaagtt cctcatcaaa
     1021 acaaatcagc aagggctgac ggtatccatt acgaacagtg tgcgaacggg tttgaccatt
     1081 tgttccattc gtttctctca cttcagattc cataattcgt tgtccgccga caaagaactc
     1141 gcctttggga cccatttttg cataggaaaa aaggaattgt cgtcctctgt aggaaatacc
     1201 caatcgcttg gcttgtttgt cggcttgttg gtgaaacgaa cgctggtggt ggtaactaat
     1261 tttgtcaatg cgatcaccaa cgattcgcac aagctgaaat ccctggtatc gttttccgtt
     1321 tttcgaaatt tcatgtcgtg gttctgttgg caacgcagta taataccggt ttggttttgc
     1381 cggtccccat aacaacaagg tatcgttgcg atgcataata tccatcatct tccaaccact
     1441 tccataaaga agtaatggca tttccgctat agctccttcc tttgaaacct taacgattaa
     1501 ccagtttcct tttttgggat ctttattact gataaatgca cttccggccg gtgcaaaaac
     1561 ataaagcatt gatgcattgg gatccggaaa acgatgattg cgttcttccc caaggaaatg
     1621 ttcatcatcc gtatactcga gtttttttgc acgcaccaat tccattttat attggaaatt
     1681 ccattcacgc aagacctcca tattcagatc gcggtcgatt cgcaataact gataatgctc
     1741 aagcaaagga accctggagt gcggactttg ttgaagtaat tttccttttt ccttgaaagc
     1801 aatcaatgca aatactttat caactcccgg atgataaaac tggtgaacga gatagtattt
     1861 ctcatcctgc agtcgttcca aatc
//
LOCUS       DAOMYG010000530         1879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_24195_length_1879_cov_7.539474, whole genome shotgun sequence.
ACCESSION   DAOMYG010000530 DAOMYG010000000
VERSION     DAOMYG010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24195_length_1879_cov_7.539474"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..557)
                     /locus_tag="PK610_13395"
     CDS             complement(171..557)
                     /locus_tag="PK610_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HRJ36984.1"
                     /translation="MSNFTLDGTPKTPTVNLNADQGVLELKGRSIPENSIEFYKPIFD
                     WVESYGGNPKSDTQVNVVLEYFNTSSSKCILDFFKKLENLNNGGKTKVTINWHYEEDD
                     EDMLEAGEDYQAIISVPFKMVAIEEI"
     gene            complement(624..>1879)
                     /locus_tag="PK610_13400"
     CDS             complement(624..>1879)
                     /locus_tag="PK610_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36985.1"
                     /translation="YSKTKPMRQLLLLLLLSTQLTVSAQKADSTSNGHLNVFLDCSSC
                     DEENLRQKVAHLNYVRDRLLCDVHILITEQATGSGGTAFLLQFFGRKQFENMNDEFHC
                     DLPANSTADEQRDLLNKYIQLGLVRYMLRTPLASHLELNLDETTPDNDTVKDKWNYWV
                     FQISGGGWLNGEKYYSNTNLYGDVTANRVKKEGKTNFEFYTNYSRNVFQISETEKVIG
                     LYRQYWSNFRHVWSLSDHWSLGAGTTYFSSLYENLDHSIDVGPEVEYNIFPYDESSTR
                     MLYFSYGIKPMFRDYNRTTVLFLDEQFLVSHNLSFNTKFIKKWGQINSDVTWSNYLHD
                     FNLSRLSLSLNGQFRLFKGFSLSISAYYAMIKNLINLPLEGATEEEMLLRQVQLPTSS
                     QYWCSFGISYTFGSMYNNIVNPRLD"
BASE COUNT          555 a          373 c          393 g          558 t
ORIGIN      
        1 gtgtttctgg tagtataacg cagaaaagga taaaaatgtt atgcgtgccg actaaaaaat
       61 cactaccgtt caatcaatag gtactacagc ttgaccaaaa ccccttattt gatgacggcc
      121 ataaaatgaa aaacccgcct tttgggcggg ttttctgtaa aatatcggga tcagatttct
      181 tcgattgcaa ccattttaaa cggaaccgaa ataatggcct gatagtcttc gccggcctca
      241 agcatgtctt catcatcctc ttcataatgc cagttaatgg taacttttgt tttgccacca
      301 ttgttcaggt tttcaagttt cttgaaaaaa tcaagaatac acttggaaga agaagtattg
      361 aagtattcta atacaacgtt cacctgtgtg tcagatttgg gattaccgcc ataactctct
      421 acccaatcaa aaatgggctt ataaaattca atggagtttt ccggaatcga acgacccttc
      481 agttcaagta cgccctgatc ggcattcagg ttgacagttg gggtttttgg agtcccgtca
      541 agtgtgaagt tgctcataac tacggttttg aatcaatttg agctacgaat atacaaaagt
      601 tcccaaaacc ctgaaagaag agctcaatcc aacctcggat ttactatgtt attgtacatc
      661 gacccgaagg tgtaactgat cccaaaactg caccaatact gactgctggt aggtaattga
      721 acctgtctta acaacatttc ttcttcagta gccccttcga gtggaaggtt aatgaggttt
      781 ttgatcatgg cgtaatatgc tgatatactg agggaaaagc ctttgaaaag acgaaattgt
      841 ccattcagcg aaagactgag cctgcttaaa ttgaaatcgt ggagatagtt cgaccaggta
      901 acatcgctat tgatctgtcc ccacttttta atgaatttgg tattaaagga taaattgtga
      961 gaaaccagaa attgctcatc aaggaaaagg acggttgtac gattgtaatc gcgaaacatg
     1021 ggtttgatgc cataactgaa gtaaagcatt cgtgttgagg attcatcgta tggaaaaatg
     1081 ttgtattcca cttccggacc tacgtcaatt gagtgatcaa gattttcata cagactggaa
     1141 aaatatgttg taccagcacc cagactccaa tggtccgaaa gcgaccagac atgccgaaag
     1201 ttactccagt attgccgata tagtccaata actttttcgg tttcgctgat ctgaaataca
     1261 tttctggaat aattcgtgta aaattcaaaa ttggttttgc cctctttttt cacacggttt
     1321 gcagtaacat cgccatagag atttgtattg gaataatatt tttcaccgtt caaccatcca
     1381 cctccggaaa tttgaaacac ccaataattc catttgtcct ttaccgtgtc attgtctggt
     1441 gtggtttcat cgaggttcaa ttccagatga gaagccaggg gagtgcgaag catatagcga
     1501 accaatccga gctgaatgta cttattgagt aaatctcttt gttcatcagc agtgctgttg
     1561 gctggtagat cgcagtgaaa ttcatcgttc atgttttcaa attgctttct tccgaaaaac
     1621 tgcaaaagaa aggcagttcc ccctgagccg gttgcttgtt cggtaatcag gatgtgtaca
     1681 tcacataaca aacgatcgcg cacataattt aaatgcgcaa ctttctgtcg caggttttct
     1741 tcatcgcagg aagaacaatc aagaaagacg tttaaatgtc cgttggaggt gctgtctgcc
     1801 ttttgtgccg acacagttaa ttgtgtggaa aggagaagga gcagtaaaag ttgtcgcatt
     1861 ggtttggttt tggaatagc
//
LOCUS       DAOMYG010000531         1856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_24622_length_1856_cov_7.237646, whole genome shotgun sequence.
ACCESSION   DAOMYG010000531 DAOMYG010000000
VERSION     DAOMYG010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24622_length_1856_cov_7.237646"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1688
                     /gene="uvrA"
                     /locus_tag="PK610_13405"
     CDS             <1..1688
                     /gene="uvrA"
                     /locus_tag="PK610_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006263689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRJ36986.1"
                     /translation="NMYKIQYRVMLSRYRGKTDCPDCMGTRLRKDAQYVKVGGRSITE
                     LLFEPISRLGGFFEGMKLNQHEEKIAKRILIEIRSRLQFLHDVGLGYLTLNRNANTLS
                     GGESQRINLATSLGSSLVGSMYILDEPSIGLHPRDTQRLIKVLKSLRDIGNTVIVVEH
                     DEEVMRTADEIVDMGPMAGSLGGEVVFQGDHNDLLTAGKSMTAAYLTGKEVIPVPEKR
                     RKWKDYIELKGVRENNLKGVDVKLPIGVLTVVTGVSGSGKSSLIKRVLHPALTKHFGG
                     YGDKSGMFDGMSGSFRQLQAVEFIDQNPIGKSSRSNPVTYVKAFDEIRNLYSVQQASK
                     VKGFKPSHFSFNVDGGRCEMCEGEGSITVEMQFMADVHLQCEECKGKRYRDEVLEVQF
                     HEKSIFDILELTVDDAITFFKKHSKGKGDHESKIVARLQPLQEVGLGYVKLGQSSSTL
                     SGGEAQRIKLASFLGNEKNDSTTLFIFDEPTTGLHFHDIKKLLHALNALIDRGHSVLI
                     IEHNADVIKCADWIVDLGPEGGEDGGEIVFEGVPEDIIKNKRSYTGKHLKGKL"
BASE COUNT          581 a          357 c          445 g          473 t
ORIGIN      
        1 gcaatatgta caaaatccag tacagagtca tgcttagtcg ttaccgcggt aaaacggatt
       61 gtccggattg catgggaaca cgattgcgaa aagatgccca gtacgtaaaa gttggcggaa
      121 gatccattac agaattacta tttgaaccga taagtcgtct tggtggtttt ttcgaaggaa
      181 tgaagctcaa tcaacacgaa gaaaaaattg caaaacggat cttaattgaa atcaggagcc
      241 gcttacaatt cctccacgat gttggattag gatacctcac attaaaccga aacgccaaca
      301 cactttcagg gggagaatcc caaaggataa atcttgccac atcattaggc agcagcctgg
      361 tcggatcgat gtatattctg gatgaacctt caatcggatt gcatccacga gacacgcaac
      421 gtctgatcaa agttttgaaa tcgctgcgtg atattggcaa tacggtgatt gttgttgaac
      481 atgacgaaga agtgatgcgc actgccgatg aaatagtgga tatgggtccg atggcgggat
      541 cgctgggtgg tgaagtggta tttcagggtg atcacaacga cttgctcact gcaggaaaaa
      601 gcatgactgc tgcatatctc acaggcaagg aagtaattcc tgtaccagaa aagcgccgca
      661 aatggaaaga ttatatcgag cttaaaggag ttagggaaaa caatctgaaa ggtgtagatg
      721 tgaaactccc cataggagta ttaacggttg taaccggggt aagcggatcc ggaaaatcat
      781 cccttatcaa acgcgtactt catcctgcat tgaccaaaca ctttggcgga tatggcgata
      841 aatccggcat gtttgacggc atgagcggat cgttccggca gttacaggcg gtagaattta
      901 ttgatcagaa tccgattgga aaatcatcaa gaagcaatcc tgttacctac gtaaaagcat
      961 ttgatgaaat caggaatttg tattctgttc agcaggcatc gaaggtaaag ggattcaaac
     1021 catcacattt ttcatttaac gtagatggtg gtcgctgcga aatgtgcgaa ggagaagggt
     1081 caattactgt tgaaatgcaa ttcatggccg acgtacatct gcagtgtgaa gaatgcaagg
     1141 gcaaacgtta ccgcgacgaa gtacttgaag tgcagtttca tgaaaagagt attttcgata
     1201 ttcttgaatt gacggtagat gatgccatca ccttctttaa aaaacacagt aaaggaaaag
     1261 gagatcatga atcaaaaata gtagcccgac tgcaaccgct gcaggaagta ggattgggat
     1321 atgtaaaact tgggcaatca tccagtaccc tatccggtgg tgaagcgcaa cgcattaaac
     1381 tggcttcttt tctcggtaac gaaaaaaacg attcaactac tttgttcatt tttgatgaac
     1441 caacaacggg actgcatttt cacgatatca agaaattatt acacgccctt aatgcactca
     1501 tcgatagggg gcacagcgtg ctgataattg agcacaatgc cgatgtgata aaatgtgccg
     1561 attggatcgt cgatctgggt ccggaaggcg gtgaagatgg aggtgagatt gtttttgaag
     1621 gtgtgccgga ggatatcatc aagaacaaac gctcgtatac cggaaagcat ttaaaaggca
     1681 aactctaata cgatcatttc atttcaacat tggttcatca tgaaaaatgc acgtttattt
     1741 cttctgatat ttttggttgc tccactgatt tccggagcaa aaccaaaatt tgttgagagt
     1801 gagttgcaac tgaacaccgg caccggaatt ttaaatggta ccctttgtgt acccaa
//
LOCUS       DAOMYG010000532         1847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_24783_length_1847_cov_8.583147, whole genome shotgun sequence.
ACCESSION   DAOMYG010000532 DAOMYG010000000
VERSION     DAOMYG010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24783_length_1847_cov_8.583147"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="PK610_13410"
     CDS             <1..176
                     /locus_tag="PK610_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRJ36987.1"
                     /translation="ISILPGVKSPTIVPLAMKGWSSVHSVISEDKFWEIIDKLRDAGA
                     QGILILPIEKMIV"
     gene            183..1469
                     /gene="hisD"
                     /locus_tag="PK610_13415"
     CDS             183..1469
                     /gene="hisD"
                     /locus_tag="PK610_13415"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013752041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRJ36988.1"
                     /translation="MNYYKYPDKASLHQLLLRPEKEQQSLEATVALVFDAVKEKGDVA
                     LLEYTRKFDGADLATVRVSQNDIDASEEKIDKSLRSAMEIAMNNIAKFHSAQKEEVKM
                     IDTNPGVTCWRESKAIDKVGLYIPGGTAPLFSTVLMLGVPAKLAGCARIVLCSPPDKT
                     GNIHPAILYAAKLIGISEVFAVGGIQAIAAMTFGTETVPAVHKLFGPGNQFVTAAKQF
                     ALRKGVAIDMPAGPSEVLVVADDSADPDFVAADLLSQAEHGADSQVLLLCFSGDFVAN
                     VQSALKQQLENLPRKELARAALEHSKIIELRSVAEAIDVINEYAPEHLILQVEDEGEY
                     LENIRNAGSVFIGKYTPESAGDYASGTNHTLPTNGFARSYSGVSLDSFVRKITFQRIS
                     PKGLQSLGPAIELMAEAEELEAHKNAVTIRLKKLNA"
     gene            1466..>1847
                     /locus_tag="PK610_13420"
     CDS             1466..>1847
                     /locus_tag="PK610_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRJ36989.1"
                     /translation="MKPDPLKLIRPTIAALSPYSSARDEYKGNEGIFLDANENPFNNG
                     YNRYPDPYQWKVKQRLSEIRRIPVQNFLLGNGSDEVIDLLFRAFCVPAKDNVILLPPT
                     YGMYKVSANINDVEVREVQLDHNFQ"
BASE COUNT          512 a          388 c          436 g          511 t
ORIGIN      
        1 tcatctccat tcttccgggg gtgaagtcac caaccattgt tccgcttgca atgaaaggct
       61 ggagtagtgt gcactctgtt atttcagaag ataaattctg ggagatcatc gacaaactcc
      121 gtgatgccgg tgcgcagggc attctcattt taccgattga aaaaatgatc gtttaaatca
      181 tgatgaacta ttataaatat ccggataaag cgagtttgca tcaacttttg ttgcgccctg
      241 aaaaggaaca acagtcactc gaagcaaccg ttgcacttgt ttttgatgcc gtaaaggaaa
      301 agggagatgt tgcgttattg gaatacaccc ggaaatttga cggagcagat cttgctacgg
      361 taagggtaag tcaaaacgat attgatgctt ccgaagagaa gattgataaa tcgctgcgtt
      421 ctgcaatgga gatagcaatg aataacattg ctaaatttca ctcagcgcaa aaagaagaag
      481 tgaaaatgat cgacaccaat cccggtgtta cctgttggcg cgaaagtaag gccatcgata
      541 aagttggatt gtatattccc ggtggaacag ctccattatt ttctactgta ctaatgctgg
      601 gtgttccggc aaaacttgca ggttgtgcgc gaatcgttct ttgctctcct ccggataaaa
      661 caggaaatat ccatcctgcc attctttatg ctgcaaaact tattggtata agcgaggttt
      721 ttgcagttgg aggaattcag gctattgctg ctatgacatt cggtacggaa acggtacctg
      781 cggtccacaa acttttcgga ccgggaaatc agtttgtaac ggctgccaaa caatttgctt
      841 tgcgcaaggg agttgccatc gatatgcctg ccggtccttc tgaagttctt gtggttgcag
      901 atgattctgc cgatcctgat tttgtggcgg cagatttgct ttctcaggca gaacatggtg
      961 ccgatagtca ggttctgctg ctttgttttt ccggtgattt tgtcgcgaat gttcaatcgg
     1021 ctttaaagca acaattggaa aacctgccac gaaaagaact tgcacgtgca gctttggaac
     1081 attcaaaaat tattgaactg cgatcagttg cagaagctat tgatgtgatc aatgaatatg
     1141 cgcccgaaca tttaattctg caagtggaag atgaaggcga atacctcgaa aatatacgca
     1201 atgcaggttc tgttttcatt ggtaaataca cacccgaaag tgccggtgat tatgcttcgg
     1261 gaaccaacca tactttacca accaacggat tcgcgcgttc ctacagtggt gtttcgctcg
     1321 atagttttgt acggaagatc acgtttcaac ggatttctcc caaaggactt caatcgctcg
     1381 ggcctgcaat tgagctgatg gctgaagccg aagaactgga ggctcataaa aatgcggtga
     1441 ccatccgtct caaaaaactg aatgcatgaa acccgacccg cttaaattaa ttcgtccaac
     1501 cattgctgca ctcagtccgt attcctcggc acgcgacgaa tacaaaggga acgaaggaat
     1561 tttcctggat gcgaatgaga atccgtttaa caatgggtac aaccgctatc ccgatcctta
     1621 tcaatggaaa gtgaagcagc gactttcgga aattcggcga atacctgttc agaatttttt
     1681 gcttggcaat gggagtgatg aagtgatcga tttgctcttt cgtgcgtttt gtgttcctgc
     1741 aaaggataat gtgattctcc ttccaccaac gtacggaatg tataaggtaa gtgccaacat
     1801 caacgatgtt gaagtgcgtg aagtgcagct cgatcataat ttccagc
//
LOCUS       DAOMYG010000533         1844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_24845_length_1844_cov_7.948016, whole genome shotgun sequence.
ACCESSION   DAOMYG010000533 DAOMYG010000000
VERSION     DAOMYG010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24845_length_1844_cov_7.948016"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PK610_13425"
     CDS             complement(<1..683)
                     /locus_tag="PK610_13425"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRJ36990.1"
                     /translation="MQRKQLAYWVAQFSGWGFYIFISGFSNYLNNELSRPILAAFLAL
                     FVSGIVISHLMRTFIVHRGWLKFSLLKLFLPVVITTGFFALVLGVLQLSFSALIVGRS
                     DIFASLNFSNGFVIWLNWWMILMIWCLVYFAFHFFERSRNEEIKNLRLEAMRTEVELN
                     NLKSQLNPHFMFNSMNSIRALIDENPVIAKESITKLSNILRNTLLMGRKRLVGIEEEL
                     SLVEASLRL"
     gene            772..1380
                     /locus_tag="PK610_13430"
     CDS             772..1380
                     /locus_tag="PK610_13430"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HRJ36991.1"
                     /translation="MNFLAHALLSGNDPLVRAGNFVGDSVKGSQLQHYPVAMQKGILL
                     HRFIDSFTDTHSVVHESKSLLRQRFGKYAGVVADVIYDHYLARNWDLHHDHLDLSSYA
                     KETYSILQTNFEQLPERSKEFLPYMIRQDWLSSYKTREGISLILLRMSKRTGFVSGME
                     KAEEELEERDHIYEEHFSRFFPELRLASELELDRLNQLQWPE"
     gene            1368..1637
                     /locus_tag="PK610_13435"
     CDS             1368..1637
                     /locus_tag="PK610_13435"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRJ36992.1"
                     /translation="MARIKIDLHPIFNDTKAIDKALRDAIREAIDCKITLIEIIPGKG
                     SGALKEKVIRFLEKKEIKQLYHRIEKDPGNHGRLWVHFRFTNPRN"
BASE COUNT          582 a          379 c          371 g          512 t
ORIGIN      
        1 tctaaccgaa gcgaagcttc tacaagtgat aattcctctt cgatacccac taagcgttta
       61 cgtcccatca acaatgtgtt acgtagaata ttggagagtt tcgtaatact ttcttttgca
      121 ataacaggat tctcatcaat cagtgcacga atggaattca tgctgttgaa cataaaatgc
      181 ggattgagct gcgacttcag gttattcaat tccacttctg tacgcattgc ctcaagtcgc
      241 agatttttta tctcttcatt tctggagcgc tcaaaaaagt gaaatgcaaa atagacaaga
      301 caccaaatca ttaaaatcat ccaccaattg agccagataa caaatccgtt tgaaaaatta
      361 agtgatgcaa aaatatcaga tcgtccaact attaatgcgg aaaaactcaa ttgaagtacg
      421 ccaagaacca atgcaaaaaa tccggtggtt atgactacag gtaaaaacaa tttgagaagt
      481 gagaatttca gccatccacg gtgcacaata aaggtgcgca tcaggtgact gatcacaatt
      541 cctgaaacaa acaaggcaag aaatgcggcc agaataggtc ggcttaattc attattgaga
      601 taattgctaa agccgctgat gaaaatataa aatccccatc ccgaaaattg agcaacccaa
      661 tatgccagtt gtttccgctg caattgctta tttttgttgt aaggtacgtg cttttatttt
      721 ttgcgcagtc tgaatgtgtc cagtggttat atttctcaat aaaaggaaca aatgaatttt
      781 ctcgctcatg ccttattatc aggtaacgac cccttggttc gtgcgggaaa ttttgttggc
      841 gattcggtta aaggaagcca gcttcaacac tatccggtgg ccatgcaaaa gggtatttta
      901 ttgcatcgtt tcattgacag ttttacagac acacattcag ttgtgcatga aagtaaatcc
      961 ttgttacgtc aacgattcgg gaaatatgca ggagtggttg cagatgtcat ttacgaccac
     1021 tacctggccc gaaactggga tttgcatcat gatcacctcg accttagttc ctatgcgaag
     1081 gagacctatt ctattttgca aacgaatttt gagcaattac cggagcgctc aaaagaattt
     1141 ctcccctata tgattcgtca ggactggctt agttcataca aaacacgaga aggcatttca
     1201 ctcattttac tgcgcatgtc aaagagaacc ggcttcgtat caggcatgga aaaggcagaa
     1261 gaagaacttg aggagaggga tcatatttac gaagaacatt tcagcaggtt tttccccgaa
     1321 ttgcgattgg ccagtgagtt ggaactcgat cgattaaatc agctgcaatg gccagaataa
     1381 aaatcgacct ccaccccatt ttcaacgaca caaaggccat tgacaaggca ctacgcgatg
     1441 ctatcaggga agccattgat tgcaaaatca ccttgattga gattatcccc ggcaaaggct
     1501 cgggcgcact taaagaaaag gtaatcaggt tcttggaaaa aaaagagatc aagcaacttt
     1561 accatagaat cgaaaaagat cccgggaatc acggacgact gtgggttcac tttcgcttta
     1621 ccaatccaag aaactgatcg aaatccttcc tgtttttttg gggtttaggg taatttatat
     1681 ctacttttga ttagtaaacg agtaaattat gttcaaaaat cacatgctgc tattcatttt
     1741 tttggccggc gcaacgattt taaaggctga cggtcaggtt cttacagtag aaggacaata
     1801 ccaaaacaaa aacctttatg tatctaatgc ggttgcttct tccg
//
LOCUS       DAOMYG010000534         1818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_25372_length_1818_cov_10.092456, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000534 DAOMYG010000000
VERSION     DAOMYG010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25372_length_1818_cov_10.092456"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PK610_13440"
     CDS             complement(<1..563)
                     /locus_tag="PK610_13440"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HRJ36993.1"
                     /translation="MLNFLKKIKSILAIRAQKTVLPGFEGQNLYDVGKFFIAGLSSGS
                     VASRAAAVTFRFLLAAFPMVICIFSLIPFVPIDNFQNSIIDYMHGFFPAQVYDFFDEA
                     LVDLIKRKRTFLLSIGFLITIYFASNGIKALLEAFSSSSHLEKKRSFLQQSIWSLGIL
                     FMFILLSVLLTLISAAGQFMIDYFYRI"
     gene            complement(557..1390)
                     /gene="nadC"
                     /locus_tag="PK610_13445"
     CDS             complement(557..1390)
                     /gene="nadC"
                     /locus_tag="PK610_13445"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008636153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRJ36994.1"
                     /translation="MELTDLIRFAIREDAGDGDHTSLACIPEGTTSKAKLLVKESGVL
                     AGINSAKHIFAEIDPSLKIEWLKKDGDIVQKDDIAFFVEGSVHSILLGERLVLNIMQR
                     MSGIASITRKIVDSLQGTKCKVLDTRKTTPLLRALEKEAVRIGGGTNHRIGLYDMILI
                     KDNHVDYAGGITKALDATRNYLGKTGKNLRIEIETRNLNELAQAIQHGGFHRVMFDNY
                     SLKDLRKGVEMVAGKFETEASGGITPENAYDYAATGVDFISMGCLTHSVKSMDLSLKA
                     C"
     gene            complement(1427..>1818)
                     /locus_tag="PK610_13450"
     CDS             complement(1427..>1818)
                     /locus_tag="PK610_13450"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HRJ36995.1"
                     /translation="KTMRTLFASFLMLFATSLFAQTDITDQVVGYIKTANVKELAKHF
                     ADNIDLAVDDTDDIVSRAQAEQILKKFFDKNQPKNFAIKHSGVTKLGIEYRIGDLETS
                     GGNFRVSINLKKVGDIYQIHQFRIEPA"
BASE COUNT          494 a          387 c          393 g          544 t
ORIGIN      
        1 tcgattcggt agaagtagtc gatcatgaat tgacctgcag ctgaaatgag tgtgagtaat
       61 accgacagta gaatgaacat aaataaaatg cccaatgacc aaatgctttg ttgcagaaaa
      121 cttctttttt tctcgagatg tgatgatgaa gagaatgctt ccagaagtgc cttgattcca
      181 ttcgatgcaa aatagatggt tatcaggaat ccaattgaga gcagaaatgt tcgttttcgc
      241 ttaataagat caacaagtgc ttcatcaaaa aaatcataaa cctgtgccgg gaaaaatcca
      301 tgcatataat cgataatgct gttttgaaaa ttgtcgatgg gtacgaaggg tataagtgaa
      361 aaaatgcaaa tcaccattgg aaaggcagcc agtaaaaagc ggaaagttac cgcagctgcc
      421 cgacttgcaa cactgcccga tgatagtccc gcaataaaaa actttcctac atcataaagg
      481 ttttgacctt caaatccagg cagaaccgtt ttttgtgcgc gaatggcaag gatgcttttt
      541 atttttttca ggaagtttag catgccttca aactaagatc catcgatttt actgaatggg
      601 taaggcaacc catgctaatg aaatcaacac cggttgcagc gtagtcgtat gcgttctcag
      661 gtgtaattcc tcctgatgct tctgtctcga acttacctgc taccatttct actccttttc
      721 gaagatcttt caggctgtaa ttgtcgaaca tgaccctgtg aaatcctcca tgttgaatgg
      781 cttgtgcaag ttcattcaga ttacgggttt ctatttcgat acgcaggttt tttccggttt
      841 ttccgaggta gtttcttgtt gcgtcaagcg cttttgtaat accgccggcg taatcaacat
      901 ggttatcttt aatcagaatc atatcgtata gaccaattct gtgattggtt cctccgccaa
      961 tcctgacagc ttctttttcc aatgcgcgta gcagtggagt tgttttgcgg gtgtcaagta
     1021 ctttgcactt ggttccctga agcgagtcaa caatctttcg ggtgatggat gcaattccgc
     1081 tcatgcgttg catgatgttt aataccaaac gttctcccag tagtattgaa tggacggatc
     1141 cttcaacaaa aaaggcgata tcgtcctttt gaacgatatc gccatctttt ttaagccatt
     1201 cgatctttaa tgatggatcg atttcggcaa atatgtgttt tgcgctgttt attcctgcca
     1261 gtacaccact ctctttaacc agtaatttgg ctttgcttgt ggttccctca gggatgcagg
     1321 caagcgaggt gtgatcaccg tcaccggcat cttcccggat tgcgaagcga atgaggtctg
     1381 ttaattccat gcggcaaaac taagcattcg ccgcgaagag aggaatttat gcaggctcaa
     1441 tcctgaactg gtggatctga tagatgtcgc caaccttctt gaggttaatg ctaaccctga
     1501 aattaccacc ggatgtttcc aggtccccaa tacggtactc aattcccaat ttggtaacac
     1561 cagaatgctt gatggcaaag tttttaggtt gattcttgtc gaagaatttc ttcagaatct
     1621 gctctgcctg agcacgactc acaatatcat cggtatcgtc aactgcaagg tcgatgttgt
     1681 cggcaaaatg cttggcaagc tcctttacat tggcggtctt aatataaccc accacctgat
     1741 cggtaatgtc ggtctgtgca aacaagctgg ttgcaaagag catcaggaag gaagcaaata
     1801 aagttctcat agttttgc
//
LOCUS       DAOMYG010000535         1816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_25408_length_1816_cov_8.488359, whole genome shotgun sequence.
ACCESSION   DAOMYG010000535 DAOMYG010000000
VERSION     DAOMYG010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25408_length_1816_cov_8.488359"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="PK610_13455"
     CDS             <1..1054
                     /locus_tag="PK610_13455"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRJ36996.1"
                     /translation="SFRNAFRQEKEKPLYTRGVNPTTSLLRQKLAALEGTEDALVLSS
                     GAAAIAAAVINKVKQGDHVLCVQHPYSWTNTLLTKHLARFGVSHTFADACDTAHFLSH
                     VQPETKVIYLESPNSITFELQDLKTIAAFAKEKGIVTICDNSYASTLGQSPAALGIDI
                     VVHSATKYLNGHSDTVAGVICASKENIQSILGSEFLTLGAICSPMEAWLILRGMRTLQ
                     LRVERSSSTAHTIFQWLKTRSEIEKIYFPHDPKNKQYDLARSQMHFSGGLLSVCLKAS
                     SIQQMEKFSNTLEYFRMAVSWGGYESLQLPVCTFYHNENERHHLPWNMVRFYFGLEDA
                     DVLIRDLENAFSQWKS"
     gene            1042..>1816
                     /locus_tag="PK610_13460"
     CDS             1042..>1816
                     /locus_tag="PK610_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ36997.1"
                     /translation="MEKLIQRFIRPTNLTILLVGLIFFFCIWNLRWFHSTDRMSGVIS
                     SDGEGYYYYLPAIFIKGDLQPEKPDPDFHVKTENGNYVNKYPFGTALLILPFFFLATA
                     CSWLFGYPIDGYSAFFQASVSLAALFYFLAGIALLLKTIRQFIALKPYQEIFTLCVIA
                     FGTPLSYYVLHYPSLSHVYSFFLINLIIWLFVGWKNENQVLARTGSFLVLGIAIVTRP
                     THVLILVFLPFLFSSFSELIAFAKKYKPKGMEIFALLLPV"
BASE COUNT          486 a          408 c          372 g          550 t
ORIGIN      
        1 gtcttttcgt aatgcttttc gtcaggaaaa agaaaaacca ctttatacaa ggggagttaa
       61 tccaactact tcactcctgc gtcaaaaact ggcagcgctc gaaggaaccg aagatgcact
      121 ggttctctcc agcggagcag ctgctattgc tgcagcagtg atcaataaag taaaacaggg
      181 agatcatgtg ttgtgtgtac agcatcctta ttcatggacc aacactcttc ttacaaaaca
      241 tctcgcccgg tttggtgtga gtcatacttt tgccgatgca tgcgatacag cgcatttttt
      301 gagtcatgta caaccggaaa ctaaagtgat ctatttggaa agtccgaatt cgatcacgtt
      361 tgagttacaa gacctgaaaa cgattgctgc ctttgcaaag gaaaaaggaa tcgttaccat
      421 ttgcgacaac agttacgcca gtacgctcgg acaatcaccg gcagcattgg gaattgatat
      481 tgtagtgcat tcagcaacca aatacctgaa cggacacagt gataccgttg caggagtgat
      541 ctgcgcaagc aaagaaaata tccaatcgat tttaggcagc gaattcttga cactgggtgc
      601 tatttgttct ccgatggagg catggttgat tttgcgcgga atgcgtacgc tgcaattacg
      661 tgttgaacga agttcttcca ctgcgcatac catttttcag tggctaaaaa cccgcagtga
      721 aattgagaaa atctattttc cgcacgatcc caaaaacaaa caatacgatc ttgcgagatc
      781 tcaaatgcat ttttcaggag gattgctgag cgtttgttta aaggcatctt caattcaaca
      841 aatggaaaaa ttcagtaata cacttgaata tttccgaatg gctgtttcct ggggcggata
      901 tgaatcgctg caattaccgg tatgcacatt ttaccacaat gaaaatgaaa ggcatcatct
      961 tccctggaat atggtccgct tttatttcgg attagaagat gccgatgtgc tgattcgaga
     1021 tctcgaaaac gcgttttctc aatggaaaag ctgatccaac gatttatccg acctactaac
     1081 ctgacaattc tccttgtcgg actgatcttc tttttctgca tttggaattt acgatggttt
     1141 cattccaccg acagaatgag tggtgtgatc agcagtgatg gagaaggata ttattactac
     1201 ctgcctgcaa ttttcatcaa gggtgatctt caacctgaaa aaccggatcc ggattttcat
     1261 gtcaaaaccg aaaacggaaa ttacgtcaac aaatatccat tcggcaccgc tcttctgatc
     1321 ctccctttct tttttcttgc cacggcttgt tcctggcttt ttggatatcc catcgacgga
     1381 tacagcgcat tttttcaggc ctcggtgagt ttagcggcgc tcttttattt ccttgccgga
     1441 attgccctac tactgaaaac aattcggcaa ttcatcgccc ttaaacccta tcaggaaatt
     1501 tttacccttt gtgttattgc gtttggaaca ccgttgtcgt attatgtatt gcactaccct
     1561 tccctatcgc atgtctattc cttttttctg atcaacctga tcatttggtt atttgtagga
     1621 tggaaaaacg agaaccaagt actcgcgcgg accggatctt ttcttgttct gggaattgct
     1681 atcgttaccc gtccgacgca tgttttgatc ctggtatttc ttcccttttt gttttcctcc
     1741 ttttcagaac tcattgcatt cgcaaaaaag tacaaaccca aaggaatgga aatttttgcg
     1801 ctccttttgc cggtta
//
LOCUS       DAOMYG010000536         1809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_25572_length_1809_cov_9.089510, whole genome shotgun sequence.
ACCESSION   DAOMYG010000536 DAOMYG010000000
VERSION     DAOMYG010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25572_length_1809_cov_9.089510"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PK610_13465"
     CDS             <1..364
                     /locus_tag="PK610_13465"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRJ36998.1"
                     /translation="EVREDDNHFAWVQQQLNIALDGLSPEQMQKVVIAYEPVWAIGTG
                     LTASRDQAQEMHAFIRRYIAERFNEETANTTRILYGGSCNAENAPLLFSCPDVDGGLI
                     GGASLKLSTFLPIILAAR"
     gene            361..1197
                     /gene="prmA"
                     /locus_tag="PK610_13470"
     CDS             361..1197
                     /gene="prmA"
                     /locus_tag="PK610_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014031806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRJ36999.1"
                     /translation="MSDYIELSLKIQPLLPGREILVYELGEAGAESFEETPDGLLAYF
                     RDSQFEESSIQSCFANYSDQFSVVFSHRKIAQQNWNAEWESGFQPIVVNERCRIRAPF
                     HSADPAFADELIIQPQMSFGTGHHETTWLMANRMYQLDLQGKSLLDMGCGTGVLAILA
                     AKRGAAPISAIDIDEWSYLNTLENLELNGVEGVFVEKGEAALLDGRRFQVILANINRN
                     VLLADLPVYAKCLEKGGQLLLSGFFISDVETLKASAHQQGLVFEGMDERNNWAVLEFK
                     KD"
     gene            1199..>1809
                     /locus_tag="PK610_13475"
     CDS             1199..>1809
                     /locus_tag="PK610_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37000.1"
                     /translation="MLTRRSLVFTLILLCGSSAIFAQTQFTPSPDKFLSQLKDYLGQY
                     DKRMAKDFMDEFEKDFKKMNGTMQSEAYDMANILSARQAKPYPEFYGYFAGLQYMYRN
                     NQFSTIYPSWRAVIEKTMLAKDKKQLPDFLLFSYQFYTDGSIYKTQSTNWKATNAFYT
                     YDYDNAPKLIFEKTDIRGFAKGDSSVIYETSGILQRVRRPRRRA"
BASE COUNT          498 a          407 c          420 g          484 t
ORIGIN      
        1 cgaagtgcgt gaggatgata atcattttgc ctgggtacag caacaactga acatcgcgct
       61 cgatggatta tctcctgaac aaatgcagaa agtggtcatt gcatacgaac cggtttgggc
      121 cattggtact ggactgacag caagccgcga tcaggcgcag gaaatgcatg cgtttattcg
      181 tcgttatatt gccgaacggt ttaacgagga aactgcaaac acaacacgca ttctttatgg
      241 cggaagttgc aatgccgaaa acgcgccctt gctgttctct tgtccggatg tggatggggg
      301 actcatcggt ggtgcttcgc ttaagttatc cacattcctt cccatcattc tggctgcacg
      361 atgagcgatt acatcgaact ctcactgaaa atacaacctc tgcttccggg tagggaaatt
      421 ctggtgtacg aattgggaga agccggagcc gaaagttttg aggaaacacc agatggtttg
      481 ttggcctatt tcagagattc gcaatttgaa gaatcatcca ttcaatcttg ttttgctaat
      541 tacagcgatc agttttcagt tgtgttttct caccggaaaa tcgcgcagca aaactggaat
      601 gccgaatggg aatccggatt tcaaccaatt gttgtaaacg aacgttgccg gatacgggct
      661 ccatttcatt ctgcagatcc tgcatttgca gatgagctga tcattcaacc ccaaatgagt
      721 tttggtaccg gtcaccacga aaccacctgg ctcatggcga atcgcatgta ccaactcgat
      781 cttcagggca aatccctgct tgatatggga tgcggaacag gggtacttgc cattcttgct
      841 gccaagcgcg gtgctgcacc aatttcggct attgacatcg atgaatggtc gtacctgaat
      901 accctcgaaa atctcgaact gaacggagta gaaggtgttt ttgttgaaaa aggagaagcc
      961 gcattattgg atggccgtcg ctttcaggtt atattagcaa acatcaaccg gaatgtattg
     1021 ctcgccgatc ttcccgttta tgccaaatgc ctggagaagg gcggacaatt actgctgagt
     1081 ggtttcttta tcagcgatgt agaaacactc aaagcgtctg cacatcaaca aggcctggtt
     1141 tttgaaggga tggatgagcg taacaactgg gcggtgctcg aattcaaaaa ggactgatat
     1201 gcttactagg aggagtttag ttttcacact tattttgctg tgtggatcat cggcgatttt
     1261 cgctcaaaca caatttaccc caagtcccga taaattttta tcgcaactca aggattatct
     1321 cggtcagtac gataagcgta tggccaaaga tttcatggac gagtttgaaa aggatttcaa
     1381 aaaaatgaac ggcacgatgc agtcggaagc ttacgatatg gcaaacatcc taagtgcacg
     1441 acaggcaaaa ccctatcctg aattttatgg gtatttcgca ggactgcaat acatgtaccg
     1501 gaacaatcag ttcagcacaa tttatccatc ctggcgtgca gtcattgaaa aaactatgct
     1561 ggccaaggat aaaaaacaac tgccggattt tcttcttttt tcctatcagt tttataccga
     1621 cggttccatt tacaaaacgc aatccaccaa ctggaaagcc accaatgctt tttataccta
     1681 cgattacgat aatgcaccga aactgatttt tgaaaagacc gatatccgcg gttttgctaa
     1741 aggagacagc tctgtcattt atgaaacctc cggaattttg caacgcgtac gtcggccacg
     1801 tcgccgtgc
//
LOCUS       DAOMYG010000537         1797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_25827_length_1797_cov_7.037887, whole genome shotgun sequence.
ACCESSION   DAOMYG010000537 DAOMYG010000000
VERSION     DAOMYG010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25827_length_1797_cov_7.037887"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            406..1059
                     /locus_tag="PK610_13480"
     CDS             406..1059
                     /locus_tag="PK610_13480"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HRJ37001.1"
                     /translation="MNFNEIKEQIEVYQSQGKRLFATSSFQTHSIPMLHLLSRIDKSI
                     PVLFINTGFHFPETMSFRDQVKEQLGLNLIDVRSLVSRNLQRDTKGQFYFASDPDYCC
                     FLNKTQPVEPFLAEYDVWINGIRADQNANRKNMKTEQAAPYGSVRFHPMLDWTSKMIY
                     DYIREYKLPKHPLENQGYLSIGCEPCTRKMDLSNDRSGRWFGLNKTECGLHTDLVTK"
     gene            1065..>1797
                     /locus_tag="PK610_13485"
     CDS             1065..>1797
                     /locus_tag="PK610_13485"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase"
                     /protein_id="HRJ37002.1"
                     /translation="MRILITGGAGYIGTELTYELLKHSAQIEEIVIYDNLSRGNYNLF
                     IGMHKLSGGKVRFIQGDLLDTRKLKKALKGIDIVYHLAAKVTTPFADQDPHLFEQVNN
                     WGTAELVYALEDEPVKKFIYASSVSVYGTSSELVDIDSPINPNTFYGISKMRGEEHVN
                     RLFQKMPTYLLRLGNVYGYSKSMRFDAVINRFMFDANFQKRITINGKGEQHRSFIHID
                     KTANLLSNISVKDTLKPGRYDLVEDN"
BASE COUNT          553 a          405 c          364 g          475 t
ORIGIN      
        1 attagtattt cacttacaaa aacttcattc tccgctatag gataacgggc aacatttccg
       61 atccgtttgt gcctggtgtt ttcaggttta cggaaatgaa aaaggtcccc caaattccgg
      121 aggaccttct gtttgttcaa actctaaaca gaaacaaacc tccatcctta gcggaagaag
      181 taaaagaacc aataggataa caatgctgtg gctgttttga ttttcatttt tcaaaaatag
      241 agcggatcac aagacccctg aaccaacatt ccttccattt gccctatttg atgtgtttat
      301 ttggcgaacc acaaggaatt gcagtcaata agcgacacta catcaaatag gacaatcggc
      361 tgttttatga caggatttaa taaacaatga atctatattt gtgtcatgaa tttcaatgaa
      421 ataaaagaac aaatcgaagt ttatcagtcg cagggaaaac gtttgtttgc cacttcctcc
      481 tttcagaccc attccatacc catgcttcat ttgctgagca ggatcgataa aagcattccc
      541 gttcttttca tcaataccgg atttcatttc ccggagacga tgagttttcg tgatcaggtg
      601 aaggaacaac tcgggctcaa cctcattgat gtccgttcat tggtatcacg caatttgcaa
      661 cgcgacacca agggccaatt ctattttgca tctgatccgg attactgttg ttttttaaat
      721 aaaacccaac ccgtagaacc ctttttggcg gagtacgatg tatggatcaa cggcatccgg
      781 gcagaccaga atgccaaccg aaaaaacatg aaaacagaac aagcggcacc ttacggatcg
      841 gttcgttttc atcccatgct cgactggacc tctaaaatga tctacgatta tatccgtgaa
      901 tacaaactac ccaaacatcc cctcgaaaac caaggctacc tgagtattgg atgcgaaccc
      961 tgcacccgca aaatggattt gagcaatgat cgcagcggac gatggtttgg attgaacaaa
     1021 accgaatgcg gactacatac tgatctcgtt acaaaataac caccatgcga atacttatta
     1081 caggcggcgc cggatatatc ggaacagaat taacctatga gttgcttaaa cacagcgctc
     1141 agattgaaga gatcgttatt tacgataatc tgagcagagg caattacaat ttgttcattg
     1201 gcatgcataa actttcggga ggaaaagtgc gctttattca gggtgatctg ctcgataccc
     1261 gtaagttgaa aaaagcatta aaaggcatcg atatcgtata tcacctggcc gcaaaagtga
     1321 ccaccccatt cgccgatcag gatccgcatc tttttgaaca ggtgaacaac tggggaacag
     1381 cagaactggt gtatgcactg gaagatgaac cggtgaagaa attcatttat gcaagttctg
     1441 tttcggttta cggcacatca tcggaactgg tggacatcga ttcgccgatt aatccgaata
     1501 ctttttacgg catctccaaa atgcgcggag aagaacatgt gaaccgcctc ttccaaaaaa
     1561 tgcccaccta tttattgcga ttgggaaatg tgtacgggta ttccaaaagc atgcgattcg
     1621 atgcggtgat caatcggttt atgttcgatg ccaactttca aaaacgcatc accatcaatg
     1681 gcaaaggaga acagcaccgt tccttcattc atatcgacaa aacggcgaac ctcctttcca
     1741 atatcagtgt aaaagacacc cttaaaccgg gtcgttatga tttagtggag gataatc
//
LOCUS       DAOMYG010000538         1792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_25921_length_1792_cov_10.137018, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000538 DAOMYG010000000
VERSION     DAOMYG010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25921_length_1792_cov_10.137018"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PK610_13490"
     CDS             complement(<1..741)
                     /locus_tag="PK610_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37003.1"
                     /translation="MLRSVIFLILIVHSITSGAQSTKYALADSLMLSLMKNTPEATAV
                     FLATGVDASNIHTMMVESGNNKGNFLTRIVADSSFDAKNKMHTLTMNLYFTEALVVWD
                     MNVELSGKKWKLRSVNTRTLRSNINDPMVIQGALGMPPKFLEYFNRRDHEAMYNLFSE
                     SLQQNMPYNDFLKLAEILHEKLGFVKQQKPEAISTFLNLHPGGVSVAKSSAYLVAEKE
                     NAVLNLQIIMDSKGTFFINTFDFISGYTC"
     gene            complement(825..1649)
                     /locus_tag="PK610_13495"
     CDS             complement(825..1649)
                     /locus_tag="PK610_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRJ37004.1"
                     /translation="MDAIKNYLIEIGPVWAALIATTFTWFLTALGASLVFFFKTMHRG
                     VLDAMLGFTGGVMVAASFWSLLNPSIEWSERLYPSMPWMPAAIGFLVGALFLYGLDKL
                     LPHLHINYGKDETEGMKTTWHHTTLLVLAITLHNIPEGLAVGVMFGAAANGMDPAAFT
                     TAIALAIGIGIQNFPEGIAVAMPLRRQGVSRWKSFWFGQLSAIVEPVAGVIGAVAVIY
                     MEPLLPFALAFAAGAMIYVVVEEVIPETQRDKYTDVAVLGFIGGFLVMMILDVALG"
BASE COUNT          517 a          430 c          384 g          461 t
ORIGIN      
        1 gcaggtataa cccgaaataa aatcgaaagt attgatgaag aaggtgccct tgctatccat
       61 gatgatctgc aaattgagca ctgcattttc tttttcagca acgagatatg cagaggattt
      121 cgcgacggaa acgccaccgg gatgaagatt cagaaaagtg gaaatcgctt cgggtttttg
      181 ttgtttgacg aatccgagtt tttcatgcag gatctccgca agtttgagga aatcattgta
      241 aggcatgttt tgctgcagac tctccgaaaa aagattgtac atcgcttcgt gatctcttct
      301 gttaaaatac tcgaggaatt taggcggcat tcccaatgca ccttgaatca ccatcggatc
      361 attgatattg gaacgaagcg tacgcgtatt caccgagcgc agtttccatt ttttccccga
      421 taactccaca ttcatatccc acaccaccaa tgcctcggtg aaatagagat tcatggttag
      481 cgtatgcatt ttattttttg cgtcgaagga actgtcggct acaatgcgtg tgagaaaatt
      541 ccccttgttg tttcccgatt ccaccatcat tgtatgaatg ttgcttgcgt caactcctgt
      601 tgccagaaaa acggctgttg cttcgggagt gtttttcatc agcgaaagca tcagactatc
      661 tgccaaagcg tatttggttg actgtgcacc ggaagtaatc gagtgaacaa taaggatgag
      721 gaaaataaca ctgcgtaaca ttggtatttt ttttaccaat atacagcgtt ttcactgcag
      781 taaaaaggag tcagcccggg aaggagtaaa ttcttagcaa ccgcttaacc cagagcgaca
      841 tcaagtatca tcatcaccaa aaatccgcca atgaaaccca atactgccac atcggtgtat
      901 ttatcgcgtt gtgtttcggg aattacttct tccaccacca catagatcat tgcaccggca
      961 gcaaaagcga gtgcgaatgg aagcaatggt tccatataaa tcaccgctac ggcaccaatt
     1021 acacctgcaa ccggttctac gatagcagag agctgaccga accaaaaact tttccagcga
     1081 ctcacacctt gtctgcgcaa tggcatggcg acagctattc cttccggaaa attctgaatt
     1141 ccgataccaa ttgccaatgc aatagcagtt gtaaatgcag caggatccat tccgtttgca
     1201 gcagccccaa acatcacacc cactgcgagt ccctcgggaa tattgtgtaa ggtaatggcc
     1261 aatacaagca aggtggtatg gtgccaggtg gttttcattc cttccgtttc gtctttaccg
     1321 taattgatgt gcaagtgtgg taataattta tccagtccat acagaaaaag tgcccccacc
     1381 agaaatccga tagcagcagg catccagggc atggaaggat acaaacgttc cgaccattcg
     1441 atactcggat tgagcagcga ccaaaaactg gcggccacca tcactccgcc cgtaaagccc
     1501 agcatggcat cgagtacacc acggtgcatg gtcttgaaga aaaacaccag cgaagcaccc
     1561 agcgcagtga gaaaccaggt gaacgttgtt gcaatgagcg cagcccaaac cggaccaatt
     1621 tctatcagat aatttttaat ggcatccata cgctatttgt gttttaccaa aatgtttcgt
     1681 gtaacaagat tcgacaatgt gatcttgcgt ttatccttta actcgatcac catcgaacga
     1741 tcgaagctga aaatttcaca gatgcgcaaa cctacgccga gtgtcaatcc ct
//
LOCUS       DAOMYG010000539         1786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_26055_length_1786_cov_5.915656, whole genome shotgun sequence.
ACCESSION   DAOMYG010000539 DAOMYG010000000
VERSION     DAOMYG010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26055_length_1786_cov_5.915656"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..936
                     /locus_tag="PK610_13500"
     CDS             157..936
                     /locus_tag="PK610_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRJ37005.1"
                     /translation="MHPLTAIIVDDEDLARENLQMLLQEYCPEINVIGKAGTVDTARK
                     MIRELKPRVVFLDIRMPSGSEGFDLLEELREEKFFVVFVTAFKDYAVRAFNANAIHYV
                     LKPVDIEDLRMAVGKLVEAAKTFEAYPENYTQYASTLKTLKEDIIHNKPSGKITISHA
                     KGIKLVKDDDIRYVEADGNCSMLHFTDGSRYLDTRTLGVYEEMLNPAKFYRIHKSHII
                     NMNELVEYISEDGYYAVLKGGVKLPVARNRVPDFTQRMKNM"
     gene            1157..>1786
                     /locus_tag="PK610_13505"
     CDS             1157..>1786
                     /locus_tag="PK610_13505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRJ37006.1"
                     /translation="MKIRSILVDDEFYCRNNLKMIIDDFCPEVEVVGMADSAEQARQL
                     ISEKKPDLVFLDIKMPKEDGFSLLDSMPVRDFSVVFTTAHSEFALKAFKADAVDYIEK
                     PINIDDLRNAVNKVLKYQIAQQQGGAAAQEDKHNDKENDRISLATRDGFMMVRNEEIV
                     HLEASDNYTMIYMTENRKFLSCKNIKVYEESLNKGSFFRVHKSHIINIAN"
BASE COUNT          547 a          337 c          385 g          517 t
ORIGIN      
        1 gtcaatcttt ttcatatgct ttggtttggc gacaaacaat tttactccga ttactactaa
       61 taggttgagg ctgtctagtt tacggaatcc attttggggt gtgcggtagc atttgacaaa
      121 aaaatggtta cattcgaacc tctctcttgt tgcgctatgc atccacttac agcaattatt
      181 gtcgacgatg aagatctcgc acgcgaaaat cttcagatgc tccttcagga gtattgtccg
      241 gaaataaatg taattggaaa agcaggcaca gtggatactg ccagaaagat gatccgtgaa
      301 ctcaaaccac gagtcgtctt tctggatatt cgtatgccgt caggatccga aggattcgat
      361 ttattggaag aattgcggga ggagaaattc ttcgttgttt tcgtaaccgc atttaaagat
      421 tatgccgttc gtgcatttaa tgccaatgcc attcattacg tgttaaaacc ggttgatata
      481 gaagacctac gaatggccgt tggtaaattg gtagaagcgg caaaaacctt tgaagcgtat
      541 cccgaaaatt atacgcaata cgcctccacc ttaaaaacac tgaaagagga tatcattcac
      601 aacaaacctt ccggcaagat tactatttct catgcaaaag gaattaagct tgtaaaagat
      661 gatgacatcc gttatgtgga agcagacgga aattgcagca tgttgcattt taccgatgga
      721 tcacgttatc tcgatacccg cactttaggt gtttatgaag agatgttgaa tcccgcaaaa
      781 ttttaccgca ttcataaaag tcatattata aacatgaatg agctggttga atacatttca
      841 gaagatggct attatgccgt cttaaaaggt ggagtaaaac ttcctgttgc gcgtaaccgg
      901 gtccccgatt ttacccaacg catgaaaaac atgtgattgc tttggtgggt gatttgacat
      961 ccattaatcc ttaaatttcc tgcggtactt ttttatggat caacgaccaa ggaattgtgc
     1021 tgttttgtga taattccgat ttaatgtatt gaaaataaaa cacttccatt caatcaatga
     1081 ctactacatt tcagtagttc atgaaaaccg aaaggctttc gtcgcgtaaa ttcggactga
     1141 atcaaaacgt gatcctatga aaatccgatc tatccttgtt gacgatgagt tctattgcag
     1201 gaacaatttg aaaatgatca tcgacgactt ttgcccggaa gtagaagttg ttggcatggc
     1261 agattctgcc gaacaggcac gccagctgat cagtgaaaag aagccggacc ttgttttcct
     1321 ggatataaaa atgccaaaag aagatggttt ttcgttgttg gattcaatgc cggtgcgcga
     1381 tttttcagtt gtgtttacca ctgcgcattc agagttcgcg ttaaaagcat ttaaagcgga
     1441 tgctgtagat tatatcgaaa agcccattaa catcgatgat ctgcgcaatg ctgtgaataa
     1501 agtattaaag taccagatag cgcaacaaca aggcggagcg gctgctcagg aagacaagca
     1561 taacgataag gaaaatgacc ggatttcatt ggcaactcgt gatggcttta tgatggttcg
     1621 aaacgaagaa attgttcacc tggaggcatc ggataattac acgatgatct acatgaccga
     1681 aaaccggaaa tttctttcct gcaagaacat taaggtgtac gaagaatcac tcaataaggg
     1741 tagttttttc agggttcata agtcgcatat tattaatatt gcaaat
//
LOCUS       DAOMYG010000540         1743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_26999_length_1743_cov_7.587085, whole genome shotgun sequence.
ACCESSION   DAOMYG010000540 DAOMYG010000000
VERSION     DAOMYG010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26999_length_1743_cov_7.587085"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PK610_13510"
     CDS             <1..338
                     /locus_tag="PK610_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37007.1"
                     /translation="LYEDPSQLWHSSQWSNFGANFCGFGNAKSDSLIDACNREMNDSL
                     YVVKIRELQKMVADEQPYVFLCSPQARVAIHRRFAAGIYSEKPQMFVNEFQLLSGGGG
                     TAIKPDLTQ"
     gene            335..>1743
                     /locus_tag="PK610_13515"
     CDS             335..>1743
                     /locus_tag="PK610_13515"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ37008.1"
                     /translation="MMGYILKRLLLFIPTLFVISLLAFIISVKAPGDPVEILSGSASV
                     QGGSTQHGVSKSQKDSIRLRLGLDKPLFYFSMTSSIECDTLYKIQDKKHREVVQRLTY
                     RCGDWQLVSNWYRELKDSYEWWSALEEADFKAEKDSIALEVQNAIDWKNTGNAIFLTM
                     LNSYEPKVIYAQLDSLQLLAGKVPGNLLKTRLDNLDRFWDEMYYSSPGFSFWIPSFHW
                     YGSDNQYHRWLSRLLIHGDFGYSYVDKEPISEKIWKKFFISFKLIFLSVLIAYLLSIP
                     LGVHAASKRGKLSERISSLFVFMLYSLPNFFVGMILLYLFANPDFVVWFPESGYMDPN
                     NYDPNWNFLQRWSAELPYMILPLITYTYSSFAFISRILRAGMLDTLQQDYVRTARAKG
                     IQEHNVLWKHALRNSLLPLITMFVNIFPMAIGGSVIIETIFTYPGMGLASYEAIHNYD
                     YPGIVAIFTLSGFLTMSGYL"
BASE COUNT          465 a          315 c          388 g          575 t
ORIGIN      
        1 cattgtatga ggatccttct caattatggc attcttcgca atggtcgaac ttcggggcaa
       61 atttttgcgg atttggaaat gcaaaaagtg attcgctcat cgatgcctgc aacagggaaa
      121 tgaatgactc tctgtacgtg gttaaaataa gggaattgca aaaaatggtt gctgacgaac
      181 agccatacgt attcctgtgt tcacctcagg caagagtggc aattcaccgc cgatttgctg
      241 caggtattta cagcgaaaaa ccgcaaatgt ttgttaatga atttcaattg ctgtcgggtg
      301 gaggaggaac agcaattaaa ccggatctga cacaatgatg gggtatattt taaagcgact
      361 ccttttgttt atcccgacat tgttcgtgat tagtctgctt gcatttatta ttagcgtaaa
      421 agcacctgga gatccggttg aaatcctttc gggttctgca tctgttcagg gagggagcac
      481 acaacatggc gtttctaaat cgcaaaagga ttccatacgc ttacgccttg gtctcgacaa
      541 acccttgttt tatttttcca tgacatcctc tatcgaatgc gatacgctct ataaaataca
      601 ggataaaaaa cacagggaag ttgtgcaacg tttaacctat cgttgtgggg attggcaatt
      661 ggtttcaaat tggtacaggg agttaaagga ttcgtacgaa tggtggtcag ctcttgagga
      721 agcggatttc aaagcagaaa aggatagtat tgcattagaa gtgcaaaatg ccattgactg
      781 gaaaaacaca ggcaacgcga tttttctgac catgcttaat tcgtatgagc caaaagttat
      841 ttatgcccaa ttggatagtc tgcagctttt agcaggaaaa gttccgggta atctgttaaa
      901 aacaaggctg gataatcttg atcggttctg ggatgagatg tattattcaa gtccgggttt
      961 ttctttttgg ataccctctt ttcattggta cggaagcgat aatcagtacc atcgttggtt
     1021 gagtcgactg ctcattcatg gcgattttgg atattcttat gtagataaag agccgatatc
     1081 ggaaaaaatc tggaagaagt tttttatttc gtttaagttg atttttttat ctgtgttgat
     1141 tgcatacctg ctttccattc cgctcggtgt acatgccgct tctaaaagag gaaagctatc
     1201 tgaacgaata agttcattgt ttgtattcat gttgtattct ttgcccaatt tttttgttgg
     1261 gatgatttta ttgtatttgt ttgccaatcc tgattttgtg gtttggttcc ctgagtcagg
     1321 atatatggat cccaataatt atgaccctaa ttggaatttc cttcagcgtt ggtctgctga
     1381 gttaccatat atgattcttc ctctcatcac ttatacgtat agttcatttg catttatcag
     1441 ccgtattctt cgagccggta tgttagatac tttgcaacag gattatgtgc gcactgcccg
     1501 tgctaaggga attcaggaac acaatgtgtt gtggaaacat gcattgcgca attccttatt
     1561 gccgctcatt acaatgtttg tcaatatttt tccaatggca attggaggat cagtgattat
     1621 tgaaaccatt tttacctacc cgggaatggg attggcaagt tatgaggcaa ttcacaatta
     1681 tgattatccg ggaatagttg ccattttcac tctttcaggt tttctgacta tgagcggata
     1741 tct
//
LOCUS       DAOMYG010000541         1733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_27226_length_1733_cov_9.768772, whole genome shotgun sequence.
ACCESSION   DAOMYG010000541 DAOMYG010000000
VERSION     DAOMYG010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27226_length_1733_cov_9.768772"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PK610_13520"
     CDS             <1..531
                     /locus_tag="PK610_13520"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRJ37009.1"
                     /translation="DGFDRGNGNWNRDQDMAFAFKNSTVWYYQQLARKIGWVKMEEHI
                     KSIGYGNKNIDGGLEDFWIKGKLRISAKEQVELLKKLQADMLPFKKEHMLTVKKIMIQ
                     EETENYILRAKTGWGEQDGKNIGWYVGYVEKPRKGSKEKQSDFWYFALNIDMDANRPE
                     SRMVIVKRVLAELKVI"
     gene            complement(536..1042)
                     /locus_tag="PK610_13525"
     CDS             complement(536..1042)
                     /locus_tag="PK610_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37010.1"
                     /translation="MDYKLLEGHLNKSASLKLCKWALKDRSHFDELVREVLKNEHPRS
                     GVASWALSEAARQKPEWILKHTGKLLHLLVEKNPHHALCRNVFRIYEAVLVPEKHQSL
                     AFDLAIKVVADPKRTIAERAFAMIMGQKITEVHPDLKRELEMIVEPLLENGSAGLKVR
                     ARRILKGR"
     gene            1204..>1733
                     /locus_tag="PK610_13530"
     CDS             1204..>1733
                     /locus_tag="PK610_13530"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRJ37011.1"
                     /translation="MEKIYLAGEFIATSQQIVVTDPYSGKSVASVCLASPSDVDTAIA
                     KANAVRTQMQDLPVFERAGILRKIAETFTKRREELAKLLISESAKPWRYAIAEVDRAA
                     ETFLIASEECKRFPGEVMSLDWTPSGKNKKGIVDYFPIGVIAGITPFNFPFNLVAHKV
                     APAIAAGCPIILKPASS"
BASE COUNT          490 a          347 c          407 g          489 t
ORIGIN      
        1 gatggatttg atcgtggcaa cggcaactgg aacagggatc aggatatggc ttttgcattt
       61 aaaaacagca ccgtgtggta ttaccagcag cttgcccgaa aaattgggtg ggtgaagatg
      121 gaagaacaca taaaatcgat tggttacgga aataaaaata tcgacggagg acttgaagat
      181 ttttggatca agggtaaact gcgaatcagt gccaaagaac aagtggaatt actcaaaaaa
      241 cttcaggcag atatgctacc cttcaaaaag gaacatatgc tcactgtgaa aaaaataatg
      301 atccaggagg aaacggaaaa ttatattttg cgggctaaaa cagggtgggg cgaacaggat
      361 ggaaaaaata tcggttggta tgttggttat gttgaaaagc cccgaaaagg ttccaaggaa
      421 aagcaaagtg acttctggta ttttgcactg aatatcgata tggatgccaa ccgtcccgaa
      481 tcacgtatgg taatcgttaa acgtgtgttg gccgaattga aagtgatctg attgcttatc
      541 ttcctttcag aattcttctt gccctgactt ttagccccgc actgccgttt tcaagtaagg
      601 gttcaactat catttccaat tcacgtttta agtcggggtg tacttccgtt attttctgtc
      661 ccatgatcat cgcaaaagca cgttctgcaa tggttcgctt ggggtcggca accaccttga
      721 tcgcaaggtc gaatgccaat gactgatgtt tttcgggtac gaggacagct tcatagatcc
      781 gaaatacatt tctgcaaagg gcgtgatgcg gatttttttc tacaagcaga tgtaatagtt
      841 ttccggtatg ctttagaatc cattccggtt tttgacgggc ggcttcactt aatgcccacg
      901 atgccactcc cgagcgggga tgttcgtttt ttagcacttc ccgaaccaat tcgtcaaaat
      961 gagatcgatc tttcaatgcc cacttgcaaa gtttgagcga tgctgattta ttgaggtgcc
     1021 cttcgagcag tttgtaatcc attttttcaa aaatagggaa attcacaatt gcgctgaaga
     1081 atggggtact gatatcaatc atctgtaaac cggaggtgaa aagttatggg cagttggtac
     1141 gacatatgaa cgattgtttg ttctgcattt gaattcggtt taaattggag cagtatttct
     1201 accatggaaa aaatttactt agccggtgag ttcattgcca cctcccaaca gattgtggtg
     1261 accgatcctt attcaggtaa atcggttgct tcggtttgtt tagcatcgcc ctctgatgtg
     1321 gatactgcaa ttgcaaaggc gaatgcagtg cgcacccaaa tgcaggatct ccctgttttt
     1381 gaacgtgcag gaatcttgcg caaaatcgca gagacattta caaagcgaag agaggaattg
     1441 gcaaagcttc tcatcagcga atccgcaaaa ccatggcgtt atgccattgc agaagtagat
     1501 cgtgctgcag aaacctttct gatagcctct gaagaatgca aacgttttcc gggtgaggta
     1561 atgagtcttg actggacacc ttccggaaaa aacaaaaagg gaattgtaga ctattttcca
     1621 atcggtgtta ttgccggaat tacaccattc aattttccat tcaatctggt tgcccataaa
     1681 gtagctcctg ccattgccgc cggttgtccg attattctga aaccggcatc ttc
//
LOCUS       DAOMYG010000542         1733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_27228_length_1733_cov_8.246126, whole genome shotgun sequence.
ACCESSION   DAOMYG010000542 DAOMYG010000000
VERSION     DAOMYG010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27228_length_1733_cov_8.246126"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PK610_13535"
     CDS             <1..836
                     /locus_tag="PK610_13535"
                     /inference="COORDINATES: protein motif:HMM:NF016602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37012.1"
                     /translation="ATTSSSAGAAVTPCAMATQTHQGKDKPLHYQLNKERFSAFHDRI
                     IHVVVDTYPENPNGDSWVLEKHQRNSIAIGLKDAKPDDVVLISDVDEIPGPEVIVREK
                     NSSGVRIFRQRMFYYYLNCINATEIGKHPYFWNGTIMVNKSDIKGSIQDFRELGMKLL
                     SYFHPKPLHRFYWRMKLRSEMSALNVRFVEQGGWHFSYLGGVDRIITKLEAFAHSEYN
                     KPEYKDPERIRKAIASGEDIFGRGFRYRFVPIDSTWPSYIVQHRDKYAALMNDDSKTH
                     A"
     gene            829..>1733
                     /locus_tag="PK610_13540"
     CDS             829..>1733
                     /locus_tag="PK610_13540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ37013.1"
                     /translation="MHDFPRIGFLTATDPHDKRSWSGIHSVMLEELKKRFCHVEVLGP
                     LDGGLPLKLGKIRNRLTRMFTGKAFDYSHSERLSKHYAKLVAEKIRDKKIDVLIAPTG
                     STLVAHLELSIPYALISDTTFANMVDYYPAYTSLSTSSLIQSHETEDKAIQNALFCAY
                     PGQWAATSAIRDHGAQSEKVFRIPFGANLRNVPERKQALTIRDNSVLRLLFLGVDWQR
                     KGGPLVLETFRLLQAHNIPVQLEIIGCDPGISNEGITVVPFVNKNDADGEKRITDALL
                     RRHFLFLPSVAECYGIVFCEAAACGT"
BASE COUNT          463 a          354 c          418 g          498 t
ORIGIN      
        1 cggccacgac ttcttccagt gccggcgccg ctgtcacgcc ctgcgcaatg gccacgcaaa
       61 cacatcaggg aaaggataag cctttgcatt accaactcaa taaggagcgc ttcagtgcat
      121 ttcatgatcg gatcattcat gttgtggttg acacctatcc tgaaaatcca aatggagatt
      181 catgggtact ggagaaacat cagcggaatt caattgcaat cggattgaag gatgcaaagc
      241 cggatgatgt ggtattgatc tccgatgtcg atgaaattcc ggggccggaa gtgatagtgc
      301 gcgaaaagaa ttcatcaggt gttcgcattt ttcgtcagcg catgttttat tattatctca
      361 attgcatcaa tgcaacggaa atcggaaagc atccctattt ctggaatgga accattatgg
      421 tgaataaatc ggatattaaa ggatcaattc aggattttcg tgaattggga atgaaacttt
      481 tgagttattt ccacccgaag cctttgcatc ggttttactg gagaatgaaa ttacgaagcg
      541 aaatgagtgc cttaaacgtg cgatttgttg agcagggtgg ctggcatttt tcctatttgg
      601 gtggagtcga tcgcatcata acaaaacttg aggcatttgc acacagcgaa tacaacaagc
      661 ccgaatacaa ggatccagaa cgcatccgaa aggccattgc gtcgggcgaa gatatttttg
      721 gtcgtggatt caggtaccgt tttgtgccaa ttgattctac atggccatca tatatcgttc
      781 agcatcgcga taagtatgcg gcattgatga acgacgattc gaagacgcat gcatgatttt
      841 ccacgcatag gttttcttac agctactgat ccgcatgata aacggtcgtg gtcgggaatt
      901 cattccgtaa tgctggaaga attgaaaaag cgtttctgtc atgttgaagt tttaggtccg
      961 ctggatggtg gtttacctct aaagctgggg aaaatccgta accgtcttac gcgcatgttt
     1021 accggtaaag cattcgacta ttcccattct gaacgcttaa gtaaacatta tgcaaaactg
     1081 gttgccgaga agataaggga taaaaagatt gatgtactta tcgctcctac cggatcaaca
     1141 cttgttgctc acttggaact ttccattcct tatgcgttga tttcggacac tacgtttgca
     1201 aacatggtcg attactatcc ggcctatacc tcattgagca cttcatcatt gattcaatcg
     1261 catgagacgg aagataaagc cattcagaat gcattattct gtgcttatcc gggacagtgg
     1321 gcggccactt ctgccattcg cgatcacggt gctcagagtg aaaaggtgtt tcgcattcca
     1381 ttcggtgcta atctgagaaa tgtaccggaa cgaaaacagg cgctgaccat tcgtgataat
     1441 tcggttttgc gtttgttgtt tttaggtgtc gattggcaac gcaaaggtgg tccgttggtt
     1501 ttggaaacct ttcgtttact tcaggctcat aatattccgg ttcagctgga gattatcggt
     1561 tgtgatcccg gaatcagcaa tgaaggtatt acagttgttc ccttcgtaaa taaaaacgat
     1621 gccgatgggg aaaagaggat aaccgatgca cttttaagaa gacattttct ttttttaccg
     1681 tccgttgcag agtgttacgg aattgttttt tgtgaggcag ctgcctgcgg aac
//
LOCUS       DAOMYG010000543         1728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_27355_length_1728_cov_7.824268, whole genome shotgun sequence.
ACCESSION   DAOMYG010000543 DAOMYG010000000
VERSION     DAOMYG010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27355_length_1728_cov_7.824268"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PK610_13545"
     CDS             <1..265
                     /locus_tag="PK610_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37014.1"
                     /translation="VISSHTFGMYAFSIISGSPLYKVVFLFPDEKHYEKTKHPESRNY
                     WFGKWAEQQHCSIHRGFLREGRVFRKCSYPYAKKKGRPIASPI"
     gene            complement(281..1537)
                     /gene="metK"
                     /locus_tag="PK610_13550"
     CDS             complement(281..1537)
                     /gene="metK"
                     /locus_tag="PK610_13550"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRJ37015.1"
                     /translation="MPYLFTSESVSEGHPDKVADQISDALIDNFLAYDPASKVACETL
                     VTTGQVVLAGEVKSKAYLDVQDIARDVIRQIGYTKAEYMFEANSCGILSAIHEQSPDI
                     NQGVEKKDPWKQGAGDQGMMFGYACRETDTYMPLPLELAHLLLRELATIRREQKVMKY
                     LRPDAKSQVTIEYNDNNQPVRIDAIVISTQHDDFGKEKAMLDQIKKDVINILIPRILK
                     KLPKRVQALFNDKIKYHINPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSG
                     KDPSKVDRSAAYATRHIAKNIVAAGLCDEALVQVAYAIGVAEPVGLYVNTYGTSKVNM
                     TDGEIAKKLQKIFDMRPYAIETRFALRTPIYLETAAYGHMGREPKKVTKVFNKGKDNE
                     KKVTVELFPWEKLDFVSKVKAEFKLK"
BASE COUNT          446 a          378 c          395 g          509 t
ORIGIN      
        1 ggtgatttcg tctcatacgt ttggtatgta tgcgttttcg atcatttccg gcagcccctt
       61 gtataaagtg gtttttcttt ttcctgacga aaagcattac gaaaagacga aacatccgga
      121 aagcagaaat tactggtttg gtaaatgggc ggagcaacag cattgttcca ttcatcgtgg
      181 ttttctccga gaaggaagag tatttcggaa atgttcttat ccatatgcaa aaaaaaaggg
      241 gagaccaata gcctccccaa tttaggaatt tatatcatgc ttatttcagc ttgaattcag
      301 cttttacttt gctcacgaaa tccagtttct cccatgggaa aagctcaacc gttacttttt
      361 tctcgttgtc cttgcctttg ttgaacactt tggtcacctt tttgggttcg cgacccatgt
      421 gtccgtaagc agcagtttcg agataaatcg gagtacgaag tgcaaaacgt gtttcgatgg
      481 cataaggacg catatcgaaa atcttctgaa gtttcttcgc aatttcaccg tcggtcatat
      541 tcactttcga agttccgtat gtattaacat acaaaccaac aggctcagca acaccaatag
      601 cataagcaac ctgaactagt gcttcatcgc acaatcctgc tgcaacgatg ttcttcgcga
      661 tgtgacgcgt tgcataagct gcagagcggt ctaccttgct gggatcttta ccggagaatg
      721 ctccaccacc gtgggcacct tttccgccgt aggtatcaac gatgatctta cgacctgtaa
      781 gacccgtatc accgtgtggg ccaccaatta caaactttcc ggttggattg atgtggtact
      841 tgatcttatc gttgaaaaga gcctgaacgc gcttgggtaa tttttttagg atgcgcggaa
      901 tgagaatgtt gatcacatct ttcttgattt gatccaacat cgctttttcc ttgccaaaat
      961 catcgtgctg tgtggaaatt acgatcgcat cgatacgcac aggctggttg ttgtcgttgt
     1021 attcaatggt aacctgactc ttggcgtcgg gacgaaggta tttcatcact ttctgctcac
     1081 ggcgaatagt cgcgagttcg cgcaataata ggtgagcaag ttcaagagga agtggcatgt
     1141 aggtgtccgt ttcgcggcat gcgtaaccga acatcattcc ctggtcgcct gcaccttgtt
     1201 tccatggatc ttttttctca acaccctggt tgatatcggg cgattgctca tgaatcgctg
     1261 aaagaattcc gcaagagttg gcttcgaaca tgtattctgc tttggtgtat ccgatctgac
     1321 ggatgacatc gcgtgcaata tcctgaacgt cgaggtacgc tttcgatttt acttcaccgg
     1381 caagaacaac ctgaccggtt gtaaccaatg tttcgcaggc aacttttgaa gccggatcgt
     1441 aagcaagaaa attatcgatc agtgcatcag aaatctggtc tgcaactttg tcggggtgtc
     1501 cttcagaaac ggactcagat gtaaataaat aaggcatatt tagattgttt tttgactacg
     1561 cgggcaaaag taagaaaagg aaacggtttg aagcactaca gcataaattt gtttttcagt
     1621 tgtaatcact tgaaagcaag ggttgtgcgc taaatgtgcc gacgaagctg tattctccgc
     1681 ctaaggaaag ctacttaccg gaaaagggtt ccctgagctg accgatat
//
LOCUS       DAOMYG010000544         1722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_27500_length_1722_cov_9.168566, whole genome shotgun sequence.
ACCESSION   DAOMYG010000544 DAOMYG010000000
VERSION     DAOMYG010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27500_length_1722_cov_9.168566"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1722
                     /locus_tag="PK610_13555"
     CDS             <1..>1722
                     /locus_tag="PK610_13555"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRJ37016.1"
                     /translation="LLGKSIQSFLLDSNDCFWICYQDTSGISFFDPAQNTFTHYLPDS
                     TRKDAAPPEMIVGFRQDSKKRLWVMTWDGGLYLLNKEKGTFKNWRRNFEKEWDATQPV
                     GNRVKVMNELPDGRFMLGFFSGGAPAGPPMFFDPEKEEFSFVPLDGYRFGTHPDEERF
                     VRVALTIVHFIHTDEKGDYWVGTYSGLLHIHHASKTIRRVTGITQDVIRQNHENARSI
                     LVDERGHYWIATGSTGIMVVNPKNYDVKYIRHYPLNRGSVSDNRVYGMRKDREGNIWL
                     STGAGMLDIYSPVQHQFRILPWEDMGLDYSNRSAQVIPLNQMSVRSSQQVYLSNQNGL
                     AILNAETGVVEQNLDARKIISGPKTEPVFHQFRFLGDSIFFFSKENIALMDGSGRKIV
                     QADWAKFHWRNLLFTNNSTDANDLWVGGKTNKNSGFIVKYNPGTLQALDSIYLPKGSA
                     FTGTFSYVLPSNRWMISMGSKMFGIVDPKEKSCTLFSPDSGYSYFPDSTIITACVTSD
                     GKVYITSENGVYRFDEVSGKTELLNEQIGISNEAVYAIIQDKQGVFWMALRHDILRWD
                     PKEKSSFR"
BASE COUNT          474 a          339 c          411 g          498 t
ORIGIN      
        1 cctgctcggg aaatccattc aaagttttct gctcgattcg aacgattgct tttggatctg
       61 ttatcaggat acttcaggaa tcagtttttt tgatcctgcg caaaatacct tcacccatta
      121 tttgcccgat agcacgcgaa aggatgcagc tccccccgaa atgattgtcg gttttcgtca
      181 ggattcaaag aagcgtttgt gggtgatgac ctgggacggt ggtttgtatt tgctgaataa
      241 agaaaaggga acattcaaaa actggcgacg caattttgaa aaggaatggg atgcaacaca
      301 gccggtaggt aacagggtaa aagtgatgaa tgagctgccg gatggaagat ttatgctggg
      361 ctttttcagt ggaggagcac cagccggtcc acctatgttt ttcgatccgg aaaaagaaga
      421 attcagtttt gttccgctgg atggttatcg tttcggaaca catcctgacg aagaacgatt
      481 tgttcgcgtt gctcttacca ttgtgcattt tattcatacc gacgagaagg gcgattactg
      541 ggttggtacc tatagcgggt tgctgcacat tcatcatgcc agtaaaacaa ttcgtcgcgt
      601 tacgggaatt actcaggatg tcattcgtca gaaccatgag aatgcccgtt caattcttgt
      661 tgatgaacgt ggtcattact ggatcgctac aggtagcaca ggaatcatgg tggtgaatcc
      721 caaaaattac gatgtaaaat acatccgaca ctatccgctc aatcgtggtt cagtttccga
      781 taatcgtgtg tacggaatgc gtaaggatcg cgagggaaat atctggctga gtacaggtgc
      841 aggcatgctc gatatctatt ctccggtgca gcatcagttt cgcattcttc cctgggagga
      901 tatgggactc gactattcca accgatctgc acaggttatc ccgctaaatc agatgagtgt
      961 gcgttcttcg caacaggttt atctgtccaa tcaaaacggc cttgctattc tgaatgcaga
     1021 aaccggtgtt gttgaacaaa atctggatgc gcgaaaaatt ataagtggac caaagacaga
     1081 accggtcttt catcagtttc gttttttagg tgattctatt ttctttttta gcaaagagaa
     1141 tattgcgctg atggatggaa gtggccggaa aattgtgcag gccgattggg cgaaattcca
     1201 ttggaggaac cttcttttta ccaataatag tacggatgct aatgacctgt gggtaggagg
     1261 gaaaacaaat aagaattccg gttttattgt gaagtataat ccgggcacac tgcaagcgct
     1321 cgattccatt tatcttccaa aaggatcggc attcaccgga acattttcgt atgttttgcc
     1381 ttcaaaccga tggatgattt ccatgggttc aaagatgttt ggtattgttg accccaaaga
     1441 gaaatcctgc acactgttca gtcctgattc cggctacagc tattttccgg attcaacaat
     1501 cataacggct tgtgtaactt ctgatggaaa agtttacatc acttcagaaa atggtgttta
     1561 ccgttttgat gaagtctccg gtaaaacaga attgctcaat gaacaaatag gaatcagcaa
     1621 tgaagctgtt tatgccatta tccaggacaa acaaggcgtt ttttggatgg cattacgtca
     1681 tgatatttta cgttgggatc caaaagaaaa aagtagtttt cg
//
LOCUS       DAOMYG010000545         1719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_27575_length_1719_cov_7.945312, whole genome shotgun sequence.
ACCESSION   DAOMYG010000545 DAOMYG010000000
VERSION     DAOMYG010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27575_length_1719_cov_7.945312"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PK610_13560"
     CDS             complement(<1..263)
                     /locus_tag="PK610_13560"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HRJ37017.1"
                     /translation="MKTPITYYGGKQALLKHLIPLIPSHKIYCEPFFGGGALFFAKPP
                     SEVEIINDINSEVVNFFRVAKTKFDALQKEVRASLHSRELYKKA"
     gene            294..761
                     /locus_tag="PK610_13565"
     CDS             294..761
                     /locus_tag="PK610_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37018.1"
                     /translation="MSWFSDFDKLHVTSKSAILSLVVLFPFWFVSIYLFNRPLYNQGD
                     LYIIGALCFCFSVTYFALNLLLAALAIYITDSEDEDGSATFVIGGIISVLYLSLSILF
                     CYWMEWGFKTFVFVAFLYLIIRTSVTAILAVLKSDDPTPKTGEQNSTDESTNP"
     gene            complement(765..962)
                     /locus_tag="PK610_13570"
     CDS             complement(765..962)
                     /locus_tag="PK610_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37019.1"
                     /translation="MNEKRLISTVTLLTSLCGYFYAKHIEKDAVPIVMISGFFGAVLG
                     ETIYSLINEKDDNDKHPPSAI"
     gene            complement(981..1661)
                     /locus_tag="PK610_13575"
     CDS             complement(981..1661)
                     /locus_tag="PK610_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37020.1"
                     /translation="MTNQPTKLKLRERLSLTFFFLFFCLAVVQLFRPDPMGLYEQGKE
                     SEKQYARELTQKVEHENKVYREKFDSLQRVNARLSEKVLKTDSLLTGLRYKNASLRFQ
                     LQSTIVSYNNDTSLVKNQEAFDSLANLSNEYVFHTQAQDSLCRLEILLLKEQMENRDS
                     LISICDTQVKQVEQNFYLLSKSHERQTEQLAFTEKKLKRQNFKKRLYQGAVLVLAGVC
                     TSFYLTHH"
BASE COUNT          464 a          325 c          357 g          573 t
ORIGIN      
        1 gcttttttat acagttcacg cgaatgcaac gatgctcgta cttctttctg caatgcatca
       61 aacttcgttt ttgccacccg gaagaaattc acgacctcgg aattgatgtc gttaatgatt
      121 tctacctctg agggcggttt agcgaaaaac aaagccccac caccaaaaaa aggctcacaa
      181 tagattttgt gactcggaat caatggtata aggtgtttga gcaatgcttg tttgcctccg
      241 taataggtga tgggggtctt catttattcg ctatttttgg taaaacaaaa tttatgagtt
      301 ggtttagtga tttcgacaaa ttgcacgtta cttcgaaaag cgcaatacta tcgttggtag
      361 tattatttcc gttttggttt gtttccattt atttattcaa cagaccgcta tacaatcagg
      421 gagacctata cataattgga gcattgtgtt tttgtttttc tgtcacctat tttgctttaa
      481 atcttctttt ggcggcattg gcgatttaca tcacggactc agaagatgaa gatggttcag
      541 caacttttgt aatcggtggc attatttctg ttctttattt gtccttgtcg attttattct
      601 gctactggat ggaatgggga ttcaaaacat ttgtttttgt tgcgttctta tatttaataa
      661 tacgaacaag tgttacagcc atattagcag ttttaaaatc tgatgatcct actcctaaaa
      721 caggggaaca aaattctacc gatgaatcaa caaatccata aagattatat cgctgatgga
      781 ggatgcttat cattgtcatc cttctcattt atcaaactgt aaatcgtttc accaagtaca
      841 gcaccaaaga acccggagat cattacaatg ggaaccgcat ccttttcaat atgcttggca
      901 tagaaatacc cgcatagaga agtgagcagc gtaacagtcg aaatcaatcg tttctcgttc
      961 ataaaaatta cctcctgttt ttagtgatgg gttaagtaaa atgaagtgca aactccggcc
     1021 agtaccagta cggctccctg ataaagccgc tttttgaaat tctgtcgttt gagttttttc
     1081 tcggtgaagg ccaattgctc cgtttgccgt tcgtggcttt tggaaagcaa gtagaaattc
     1141 tgttctactt gtttaacttg tgtatcacag atggaaataa gtgaatcgcg gttttccatt
     1201 tgttctttta gaagcaaaat ctccaatcgg cataaactat cctgtgcttg tgtatgaaaa
     1261 acatactcgt tgctgagatt ggcaagcgaa tcaaaagcct cttggttttt tacaaggctg
     1321 gtatcattgt tataagaaac aatggtggat tgcagttgaa agcgcagcga tgcgttttta
     1381 taccgcagcc cggtgagcag ggaatccgtc ttcagcactt tttctgaaag gcgggcattg
     1441 accctttgca gggaatcgaa tttctcccgg tagactttat tttcatgttc taccttttgg
     1501 gtaagttcac gagcgtactg cttttcggat tcttttccct gttcgtataa tcccattggg
     1561 tcaggacgga agagttgtac aacagcaaga cagaagaaca ggaagaaaaa agtcagcgac
     1621 aatctttctc ttaatttgag tttagttggt tggttcgtca ttggaatttt ggttaaaggg
     1681 ttttactttt ttttcgatgg aatacccgaa gcatccggc
//
LOCUS       DAOMYG010000546         1707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_27892_length_1707_cov_9.084746, whole genome shotgun sequence.
ACCESSION   DAOMYG010000546 DAOMYG010000000
VERSION     DAOMYG010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27892_length_1707_cov_9.084746"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1707)
                     /locus_tag="PK610_13580"
     CDS             complement(<1..>1707)
                     /locus_tag="PK610_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37021.1"
                     /translation="SYSNDGAEIGSTNFEYTSSVPLRVVNNDGTHELAFMNREVDFFA
                     DAYSTLITNDNKSIQIGGGMKNYKPFVSVFTSVNKSFEGYYFNTFTKVIEDYPILAKV
                     ESHFQGAKTISLNLAWDAQTGSPLVTKSMNDHNQSIYSVEYPSHWAYSNAKPKYLTAD
                     NHAENITITSGVANLSGTGYAATDLINVGDQIMITNSSGVNSLAWASYVSGSTVTLVD
                     RTGTKLTTTSSNNTLRVLTPNTKADPSTPMMSYITLNNPIGVSSLNAPTGILSAGALS
                     MKDCWETVSAYNFGCTPSSSECGFTEGTAINPWVTGVSGNLRPNLFYTYQVDRLENSV
                     SVTPEVDLRSGGKYNSFIPFYAYSSGAGKYVPINNPSHPNYNAGSLEKWLIASQIIHY
                     NEHGVPIESKDVLGNYSGVIFGYSNTLKILPVASAEFARYTDIASDGFEDYLFRSDLA
                     NCPNLDGHFDFKKVVSVSDLSSTEYHTGRFSLKIASGANFTVNRKLYSVSDEIPCSTP
                     QEGKGEYSASSGQNLKGFSPKTGLYQFSIWAKQNTTNLYTAVPDAKIEIKVSSPGIAS
                     IT"
BASE COUNT          497 a          344 c          335 g          531 t
ORIGIN      
        1 tggtaataga ggcaattccc ggggagctga cttttatttc aattttcgca tccggcaccg
       61 ctgtatataa attcgttgta ttctgtttgg cccaaatact gaactggtac aatccggttt
      121 taggggagaa tcccttaaga ttttgtccgg aactggccga atattcgcct ttaccttcct
      181 gtggtgtgct acatggtatt tcgtcagaaa cggaatacag ttttctgttt acagtgaaat
      241 tcgcacctga agcaattttt aaactaaacc tacctgtgtg atattcagta cttgacagat
      301 ctgaaacgga aacaaccttt ttgaagtcaa aatgaccgtc aagatttggg caattggcta
      361 agtcgcttcg gaaaaggtaa tcttcaaatc catctgaagc aatatcggta tacctggcaa
      421 attctgcaga agcaacaggg aggattttta atgtgtttga atagccgaat atcacacctg
      481 aataattacc caatacatct ttgctttcga ttggaactcc atgttcatta taatggataa
      541 tctgagaggc tatcaaccat ttttcaagcg aaccggcatt gtaattgggg tgggatggat
      601 tgttaatcgg gacatatttt cctgcacctg aagaataagc ataaaatgga ataaatgaat
      661 tgtattttcc tcctgatctt aaatctacct caggagttac acttacagaa ttttctaaac
      721 gatcaacctg gtaggtataa aataaattcg gtcgcaggtt tccagacacc ccggtaaccc
      781 atggatttat tgccgttcct tccgtgaaac cacattcact tgacgaaggt gtacatccaa
      841 agttatatgc agatacagtt tcccagcaat ccttcattga aagtgcacct gcactcagta
      901 ttccggtagg cgcatttaaa gaggatacgc caataggatt attcaaggta atatacgaca
      961 tcattggtgt tgatggatca gccttggtgt tgggagtaag aactctaagt gtattattac
     1021 ttgatgtggt tgtcagttta gttcctgttc tgtcaacaag tgttacagtt gaacctgaaa
     1081 catagcttgc ccatgcaagt gaattcacac cggaagagtt agtaatcata atctgatctc
     1141 caacattaat taagtcagtt gctgcatagc cggtacctga gaggtttgct actcccgagg
     1201 ttatagtaat attctccgca tgattgtcgg ctgtcagata tttcggtttc gcattgctat
     1261 atgcccaatg cgatggatat tcgactgaat aaatactctg attgtggtcg ttcatcgatt
     1321 tcgttaccaa aggactcccg gtttgagcgt cccaagccaa atttaaactg atcgttttgg
     1381 caccttgaaa atgactttca accttcgcaa gaatgggata atcttcaata actttagtaa
     1441 acgtattgaa ataataaccc tcaaatgact tatttactga tgtaaatacg ctcacgaaag
     1501 gcttgtagtt tttcatccct ccaccaatct gaattgattt attatcgttc gttatcaacg
     1561 ttgaataggc atcagcaaag aaatcaactt cacgattcat gaacgctaat tcatgagtgc
     1621 catcattatt aactaccctt aacggaacac tactggtgta ttcgaaattc gttgaaccaa
     1681 tttctgcccc atcatttgag tatgaag
//
LOCUS       DAOMYG010000547         1703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_28023_length_1703_cov_7.831311, whole genome shotgun sequence.
ACCESSION   DAOMYG010000547 DAOMYG010000000
VERSION     DAOMYG010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28023_length_1703_cov_7.831311"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PK610_13585"
     CDS             <1..453
                     /locus_tag="PK610_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37022.1"
                     /translation="TNKKGMLVLGSWAIGNIGIGAIGMNTSSGVDKRFHEMNLFWNTV
                     NLALAVPGRVSSVKQLRKIKEGKWVPTAEEFRKEQRKTERLFLINTGLDIVYMGTGLY
                     MNQKARTSGNPDLVKGYGNSIVLQGGFLFGFDAIMWLIHRKHRQQYVF"
     gene            complement(454..1134)
                     /locus_tag="PK610_13590"
     CDS             complement(454..1134)
                     /locus_tag="PK610_13590"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HRJ37023.1"
                     /translation="MQDNFTCLRFAITLMLSAMLMEVCAQTQDAQLWSGIEIEQKFNK
                     EHAAQLKTQFRLGDNFSRADYSYVDLGYQYSPNKVIDASAGYSLNMRNHYIRGWGSRH
                     QWYTYVTITKKIGDLKIANRNQFQSDIEDASQNEGSFFYRNRIQLRYKVNETLTPFAS
                     AEGYLRLGQRPPNENFFYRTRYIAGLHFNISKRQRIELSYLLQRQIRRDVPDYIHAYC
                     INYSITLK"
     gene            1272..>1703
                     /locus_tag="PK610_13595"
     CDS             1272..>1703
                     /locus_tag="PK610_13595"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRJ37024.1"
                     /translation="MNTQAAPDFTLNDLNGISFRLSDHLEKGFIVLFFYPKNETGGCT
                     REACAFRDRFQDFQDLGALVVGISGDSEDSHRSFAANHLLPYTLLSDPGKKVHQLYGV
                     KGGLFGLLPGRVTFIIDSGGISGRGPLRRVRCTFDAGHGGES"
BASE COUNT          476 a          334 c          401 g          492 t
ORIGIN      
        1 accaataaaa aaggaatgtt ggtattgggg agctgggcaa ttggaaatat cggtatagga
       61 gcaattggaa tgaatacgag cagtggagtc gacaaacgtt ttcatgaaat gaatcttttt
      121 tggaatactg ttaaccttgc tttggcggtt ccgggaaggg tttcttcagt aaaacaacta
      181 cgcaaaatca aagaggggaa gtgggttccc acagcagaag aatttcgtaa agaacagcgc
      241 aaaacagaac gcctgtttct gatcaatacc ggccttgata ttgtttatat gggaacaggt
      301 ctttacatga atcaaaaagc gagaacatca ggcaatcccg atcttgttaa aggctatgga
      361 aacagtattg ttttacaggg aggttttttg tttggatttg atgcaataat gtggctgatc
      421 caccgtaaac accgacaaca atacgtattc tgattactta agtgtaatgg aatagttgat
      481 gcaataagca tgaatgtaat ccggcacatc tctgcggatt tgacgttgca gtaagtagga
      541 taattcaatt cgttgtcgtt tcgaaatgtt gaaatgtagt ccagcaatgt atcttgtccg
      601 ataaaagaaa ttttcatttg gaggacgttg accgagccgc agataaccct ctgctgaggc
      661 gaagggtgta agtgtttcat ttactttgta cctcaactga attctgtttc ggtaaaagaa
      721 ggacccttca ttttgggatg catcttcaat atcactttga aattgatttc tgttcgcaat
      781 ttttagatca ccaatttttt tagtaattgt aacgtaagtg taccactgat gacgcgatcc
      841 ccaacctctg atataatgat ttctcatgtt aagtgagtag cctgcggaag cgtcgataac
      901 tttgttggga gagtactggt aaccaagatc gacataggaa taatcagcgc gcgagaaatt
      961 gtcgccaaga cgaaattggg tttttaattg agcggcatgc tccttgttga atttttgttc
     1021 gatttctatt ccactccaaa gctgagcatc ctgggtttgt gcgcatacct ccatcagcat
     1081 cgctgaaagc ataagcgtaa tagcaaaacg taaacaggtg aaattgtcct gcatggatgg
     1141 gggtatttga ctgaaggacc aagataaaga ttctgactta gtcatccaaa tgaagcaagc
     1201 gaaaacaaac ccgtttatag cttgttatca atttcgtcag cacgagcaga tatttatcta
     1261 tttttccctc aatgaacaca caagcagctc ccgattttac cctgaacgac ctaaatggaa
     1321 tctctttccg cctctctgat catctggaga agggctttat cgtattgttt ttttatccca
     1381 aaaacgaaac gggtggttgt acacgcgagg cctgcgcatt tcgtgacaga tttcaggatt
     1441 ttcaggatct tggagcattg gtggttggaa tctccggtga ttcagaagat tcacaccgtt
     1501 cttttgcagc taaccatctc ttgccctaca cacttttatc cgacccggga aagaaagtcc
     1561 atcagttgta tggtgtaaag ggcggtttat tcggactatt acccggtcgt gtaaccttta
     1621 tcatcgattc cgggggaatc tcggggcggg gcccgctgcg ccgcgtgcgc tgcacgttcg
     1681 acgcggggca cggaggcgag agc
//
LOCUS       DAOMYG010000548         1697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_28191_length_1697_cov_4.753959, whole genome shotgun sequence.
ACCESSION   DAOMYG010000548 DAOMYG010000000
VERSION     DAOMYG010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28191_length_1697_cov_4.753959"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PK610_13600"
     CDS             complement(<1..274)
                     /locus_tag="PK610_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HRJ37025.1"
                     /translation="MTAFSFSDRRSFLKKSGAGLAGMLLIPDLLRENVLHKDETRRLT
                     ILHTNDMHSRIDPFPNDDPQYAGLGGMERRAAMIASIRKAEQNVLLL"
     gene            complement(249..992)
                     /locus_tag="PK610_13605"
     CDS             complement(249..992)
                     /locus_tag="PK610_13605"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HRJ37026.1"
                     /translation="MKKSLLYLLLFIAFVSCSQWTPAVSKSGYTPVKEGKHAKSESII
                     APYRDSLSRTMKEVIGYCSNALITKKPQGSLGNFVCDLLLEDIPAIASYQFLKEHNYF
                     SLLNTRGFRAPLPEGEVTVERIFSIMPFENEVVLVRMPIKELPTIGSSLVEKGGHPLS
                     KNTSLVSFSGEFAGLHISDLKPAANMWIITTDYLAEGGDNMEFFAKAEEVISTHLKLR
                     DVIIAHIRKLTSQGKSIQAETDDRILFFR"
     gene            complement(1028..>1697)
                     /gene="aroE"
                     /locus_tag="PK610_13610"
     CDS             complement(1028..>1697)
                     /gene="aroE"
                     /locus_tag="PK610_13610"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRJ37027.1"
                     /translation="AENISDALYGNYPLAAISEVAALIENTPELAGINVTIPFKESII
                     PFLTELTPEAERIGAVNAVKIIRKQGITKTIGYNTDVYGFRMSLKPFLTSQHYRALVL
                     GTGGASKAVVYALMELGIQVATVSREKGKGDLTWAEISEPLIRNYPLIINTTPLGMHP
                     QTENYPPIPYSGIGKEHLLFDLTYNPDLTLFLTKGKAKGATVLNGLTMLKLQAEKSWE
                     IWNS"
BASE COUNT          454 a          346 c          375 g          522 t
ORIGIN      
        1 cgagtaggag tacgttttgc tcagcttttc gtatcgaagc tatcatggca gcacgacgtt
       61 ccattcctcc taatcctgca tattgaggat catcattcgg aaaagggtcg atcctgctgt
      121 gcatgtcgtt ggtgtgcaga atggtgagac gacgcgtttc atctttgtgc aaaacatttt
      181 cgcgcaaaag atcagggata agcagcattc ccgctaatcc ggcacctgat ttctttaaaa
      241 aagatcttct atctgaaaaa gagaatgcgg tcatcggttt cagcttgtat tgattttccc
      301 tgtgaagtaa gtttgcggat atgtgcaatg ataacatcgc gtaatttcaa atgcgtggag
      361 atcacttctt cggcttttgc gaaaaattcc atattgtcac caccctctgc aagataatct
      421 gttgtgatga tccacatatt ggcagcgggt ttcaaatcgc tgatgtgcaa tccggcaaat
      481 tctcctgaga aactgaccag tgatgtgttt tttgaaaggg gatgccctcc tttttctaca
      541 agcgaagaac cgatagtggg taattccttt attggcattc tgaccaacac aacttcattt
      601 tcgaagggca tgatcgaaaa aattcgctcc accgttactt caccttccgg tagcggagcc
      661 ctgaatcctc tggtatttaa gagcgagaaa taattgtgtt ccttcaaaaa ttgataggat
      721 gcaattgccg gaatatcttc caataacaaa tcacaaacaa aatttccaag cgagccctga
      781 ggttttttgg tgataagtgc attgctgcaa tacccaatca cttctttcat tgtgcgtgag
      841 agggaatctc tgtagggtgc gatgatggat tcactcttag catgtttccc ctctttaaca
      901 ggcgtgtatc cggatttgga aacagcgggt gtccattggc tgcatgaaac aaatgcaata
      961 aacagtaaaa gatataggag gctttttttc acggatgctg aatatacaaa aattcagcct
     1021 tggataatca ggagttccag atttcccagg atttttctgc ttgaagttta agcatggtta
     1081 atccgtttag tacagtagct ccctttgctt taccttttgt taaaaataaa gtaagatcag
     1141 gattatatgt aaggtcaaac agtaaatgct ctttaccaat acccgaataa ggaataggag
     1201 ggtagttttc agtttgcgga tgcattccta acggagtagt attgatgatt agcggatagt
     1261 tgcgaatcaa gggctcgctg atttcagccc aagttaaatc accttttcct ttttccctgg
     1321 atacagtggc tacttgtatg cccagttcca ttaacgcata cacaactgct tttgaagctc
     1381 cgcctgtgcc taaaacaagt gctctgtaat gctgacttgt gagaaatggt ttaagcgaca
     1441 ttctgaaacc atatacatcg gtgttgtacc cgatcgtttt ggtaatgcct tgtttgcgaa
     1501 tgatctttac agcattcacc gctccgattc tttcggcttc tggagtgagt tcggtgagga
     1561 aggggatgat tgattcttta aaaggaatgg tgacatttat tcccgcaagt tcaggtgtgt
     1621 tttcaatcag tgcagcgact tctgaaattg cagcaagcgg gtaatttccg taaagtgcat
     1681 cgctaatatt ttccgcc
//
LOCUS       DAOMYG010000549         1690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_28361_length_1690_cov_8.166361, whole genome shotgun sequence.
ACCESSION   DAOMYG010000549 DAOMYG010000000
VERSION     DAOMYG010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28361_length_1690_cov_8.166361"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..679
                     /locus_tag="PK610_13615"
     CDS             56..679
                     /locus_tag="PK610_13615"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HRJ37028.1"
                     /translation="MFFFIIGGIVFGVSFLPESWWILKFLLWFGLAVLFFLILQFFRV
                     PKRVITQNENSVICPADGKVVVIEEVMETEFFNEKRIQVSIFMSPLNVHVNFYPIGGK
                     VIYSKYHPGKYLVAWHPKSSTENERTTVVVEDEKGRKVLFRQIAGAVARRIVNYAKEG
                     NTATQSKEFGFIKFGSRVDILLPLDANVNVEINQMVQGSVTTLARFA"
     gene            complement(688..>1690)
                     /locus_tag="PK610_13620"
     CDS             complement(688..>1690)
                     /locus_tag="PK610_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ37029.1"
                     /translation="GIKEVPVKELIPGKTYFYFSHTIKKQPHNRKLLQKMIALGITMV
                     DYETLTNTSGERLIAFGRYAGIVGAYNAFRAYGEKFGVYHLKPAHECFDRKEMESELK
                     KVILPADFKIVITGSGRVGGGCLEILKGMNLQQVTSEDYLSKSFSQPVFTVLHVGDYN
                     KAKDGSVFDKKSFYGNPSAFEPDFLKWARCSDMYIPCHFWDSKAPMILRKEDYTDPQF
                     RIRVIADVSCDISIPIASTIRSSTIADPFYGYDPQTGEEADFYAPNTIGVMAVDNLPC
                     ELPRDASRDFGEVLLEKILPLLSGPDPDKIIERATMCSKGDLLPKYEYLRAYLEGKE"
BASE COUNT          489 a          370 c          344 g          487 t
ORIGIN      
        1 gcgcgtggaa gaccagcggt gcgctggcca gcgtgtcatc ggccaggtgg ggcagatttt
       61 tttttttatt attggaggaa ttgtttttgg ggtttctttt ctgccggaat cctggtggat
      121 tcttaaattt ttactttggt tcggactcgc cgttttgttt tttctcatcc tgcaattctt
      181 ccgcgttccc aaacgtgtca ttacacaaaa tgaaaacagt gttatttgtc ctgccgacgg
      241 aaaagtagtg gtgatcgaag aagtgatgga aacagaattc ttcaatgaaa aacggatcca
      301 ggtaagcata tttatgtcgc cactgaatgt tcacgtcaac ttctacccca tcggcggaaa
      361 ggtgatttat tccaaatacc atcccggaaa atacctcgtg gcatggcatc cgaaatcctc
      421 aacagaaaac gaacgcacca ctgttgtagt tgaagatgaa aaaggaagaa aagtgctctt
      481 tcgccagata gccggtgctg ttgcgcgaag gattgtcaat tatgccaaag aaggaaatac
      541 tgcgacacaa tcaaaagaat tcggtttcat aaaatttggt tccagagtag atattctcct
      601 cccattggat gcaaatgtaa acgttgagat caaccaaatg gtgcaaggtt ctgttacaac
      661 attagcacgt ttcgcatagg aataccctta ttccttacct tccagataag cgcgcagata
      721 ttcgtatttg ggtaacaaat ctcctttgga acacatggta gcacgctcaa taatcttatc
      781 aggatcagga ccgcttagca gaggtagaat tttctccaac aagacttcac cgaagtcgcg
      841 actcgcatca cgcggtaatt cacagggtag attatctaca gccatcactc cgatggtatt
      901 aggagcataa aaatctgctt cttcaccggt ttgaggatcg tatccgtaaa acggatcagc
      961 aatagtcgat gatcgtattg tagaagcaat cggaatacta atgtcacagg aaacatctgc
     1021 aattacccga atacggaatt gtggatcggt gtaatcttcc ttccgcaaaa tcatcggcgc
     1081 ttttgagtcc cagaaatgac acggaatata catatccgaa catctcgccc attttaaaaa
     1141 atccggttca aatgcagaag gatttccgta aaacgatttt ttatcgaaca cagatccatc
     1201 tttggctttg ttataatcgc caacatgcaa aacggtaaat acaggttgtg aaaacgattt
     1261 cgagagataa tcttcggatg taacctgctg aagattcatt ccttttaaaa tttccagaca
     1321 tccaccgcca acccgtccgg aaccggtgat tactatttta aaatctgcag gaagaatcac
     1381 ttttttcaat tccgattcca tttcctttcg atcaaaacat tcgtgcgcag gtttcaaatg
     1441 gtaaacacca aatttttcgc cgtaagccct gaatgcattg taagctccaa caatacctgc
     1501 atatcttccg aacgcaataa gtcgctcacc acttgtattt gttaaggtct cataatcgac
     1561 catggttata cccaaggcga tcatcttttg aagcaactta cggttatgcg gttgtttctt
     1621 gatggtgtgt gaaaaataga aataggtttt acccggtatc agctccttaa ccggtacttc
     1681 ttttattccc
//
LOCUS       DAOMYG010000550         1679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_28641_length_1679_cov_6.886084, whole genome shotgun sequence.
ACCESSION   DAOMYG010000550 DAOMYG010000000
VERSION     DAOMYG010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28641_length_1679_cov_6.886084"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PK610_13625"
     CDS             <1..628
                     /locus_tag="PK610_13625"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009419442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRJ37030.1"
                     /translation="SPYAVAQLYAFWIAKNYREAYGIFACNGILFNHESPLRGETFVT
                     RKITRAVAKIKLGLQKKVYLGNLDAQRDWGHAKDYVEGMWRMLQQDTPDDFVLATGKT
                     YSVRHFATLAFKEAGIEVEWKGEAVNEKGYRAGTNEVLVEIDPKYFRPTEVELLIGDP
                     SKAQNILGWKHNYELDGLVHEMVKADLELFSRDKYLMEGGHKVMNYHE"
     gene            636..1577
                     /locus_tag="PK610_13630"
     CDS             636..1577
                     /locus_tag="PK610_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HRJ37031.1"
                     /translation="MEKNAKIFVAGHRGMVGSAIVRRLKSMGYDGIITRTSKELDLRS
                     QSAVDDFFHSEKPEYVFLAAAKVGGIHANNVYRAEFLYDNLMIESNIIHCAYLHGVEK
                     LLFLGSSCIYPKLAPQPLKEESLLTGLLEPTNEPYAIAKIAGIKLCEAYRSQYGCNFI
                     SAMPTNLYGPNDNYDLNNSHVLPALIRKFHSAKIGNESKVEIWGSGTPKREFLHVDDL
                     AEACLFLMGNYNGELFVNVGTGLDLSIRELAEVIAEVIGFNGELVFNTDKPDGTPRKL
                     MDVSRIHAMGWKHKIDLKDGIAAVYSDVKNMEWALQA"
BASE COUNT          509 a          282 c          392 g          496 t
ORIGIN      
        1 gagtccttac gccgttgccc aactgtatgc tttctggata gcaaaaaatt accgggaagc
       61 ttatggcatt tttgcatgca acggaatttt atttaatcac gagagtcctt tgcgtggaga
      121 gacttttgtt actcgtaaaa taacacgtgc agtagctaaa ataaaactcg gcttacagaa
      181 aaaagtatat ctcggaaacc tcgatgcgca gcgtgactgg ggtcatgcta aggattatgt
      241 tgaaggaatg tggcgtatgc tgcaacagga tactcctgat gactttgttt tggcaacagg
      301 aaaaacctac agcgtacgtc attttgccac acttgcattt aaagaagcag gaattgaggt
      361 tgaatggaaa ggcgaggctg ttaatgaaaa gggataccgt gccggaacaa atgaagttct
      421 tgtggaaatc gatccgaaat attttcgtcc taccgaagta gaacttctta ttggcgatcc
      481 ttccaaagca caaaacattt tgggttggaa acataattat gaattagatg gattggtgca
      541 tgaaatggta aaggccgacc tcgagttgtt ttcgagagat aaatacctga tggaaggtgg
      601 tcataaagtg atgaattacc acgaataatt tttccatgga gaagaacgca aagatttttg
      661 ttgcaggtca tcggggtatg gtgggtagcg caatagtgcg ccgattgaaa tcaatgggtt
      721 atgacgggat tatcacccgt acatccaaag aacttgatct tcgtagccag tctgctgtgg
      781 atgatttttt tcattctgaa aaacctgaat atgtttttct ggcagcagca aaagttggag
      841 gtatacacgc caacaatgtt tacagggcag aatttttgta tgataatttg atgattgagt
      901 cgaatattat tcattgtgca tatctgcatg gagtggaaaa attactgttt ttgggttctt
      961 cttgtattta tcctaaactc gcccctcaac cacttaagga agagtcatta cttaccggtt
     1021 tacttgagcc gaccaatgaa ccttatgcta tcgccaaaat tgcaggaata aaattatgtg
     1081 aagcttatcg gtcgcaatac ggatgtaatt tcatttcggc tatgccaaca aatttgtacg
     1141 gaccaaatga caattacgat cttaataatt cacatgtatt acctgcgcta atccgtaagt
     1201 ttcattctgc caaaattgga aatgaaagca aagttgaaat ctggggctca ggaacaccta
     1261 aacgagagtt tttgcatgtt gatgatctgg ctgaagcatg tttgtttttg atgggaaatt
     1321 acaatggcga attatttgta aatgtaggaa ccggacttga tctcagtatt cgggagcttg
     1381 cagaggtaat tgcagaagta atcggattta atggtgaatt ggtttttaac accgataaac
     1441 cggatggaac tccgcgaaaa ttgatggatg tttctcgtat tcatgcaatg ggttggaaac
     1501 ataaaattga tttaaaggat ggaatagccg ctgtgtattc cgatgtaaaa aacatggaat
     1561 gggcccttca ggcttgaaat atggttgtat aatagcagcg ctattttgtg ctctgatctc
     1621 aaatgcacag cagctgaata taccgctgaa tcatactatg aatatcggtt gggaaaggg
//
LOCUS       DAOMYG010000551         1674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_28785_length_1674_cov_7.788141, whole genome shotgun sequence.
ACCESSION   DAOMYG010000551 DAOMYG010000000
VERSION     DAOMYG010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28785_length_1674_cov_7.788141"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1103)
                     /gene="mltG"
                     /locus_tag="PK610_13635"
     CDS             complement(69..1103)
                     /gene="mltG"
                     /locus_tag="PK610_13635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRJ37032.1"
                     /translation="MKRIIVIMVVGLLIGGGYYGNMLYSRMYGKNVQLKQEKTFIYIP
                     TGTDLDDLTGILMSESILEDTATFRWVAEKKNLRGKNIVPGKYEIVNGWSNNDLINHL
                     RAGNGRLEVEVTFNNVRTLEQIAGKVSKYIEADSVSIIDVLQNKDTIARYGFNRNNFI
                     SLFIPNTYKMDWATDAGEFLSVIASNYRNFWTEERKSKAKALGLSQSEVSTLASIVQA
                     EQTRHASERPRIAGLYLNRLKKGIRLQSDPTVVYAVGDFNLRRVLNKHLEVASPYNTY
                     LNAGLPPGPIQVPDITSIDAVLNYEKNDYIYMCAKPGYEGYHNFSKTLDQHEQYAREY
                     RKWLDKEGIK"
     gene            complement(1110..1622)
                     /locus_tag="PK610_13640"
     CDS             complement(1110..1622)
                     /locus_tag="PK610_13640"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ37033.1"
                     /translation="MSTPRIRLRALEPGDLDIVVRWENDPKNWPVTSTYIPFSRHLLK
                     EYVEAEKDIYRDKQFRFVIELTENNAAVGFIDLFDVDAVHQRAGVGILIGEYGERGKG
                     YAHEALMELIQHCRSILLLRSIYCNILCENTASVQLFERCGFKINGKKENWFRTSSGW
                     SDEYFLQLEL"
BASE COUNT          429 a          380 c          344 g          521 t
ORIGIN      
        1 accaatcaca cgttcttcct catcttcgtc tttcaccctt cgtagcctcg gcgaagaagg
       61 gttcgtcctc attttatccc ttccttatcc agccatttcc ggtattctct ggcgtattgt
      121 tcgtgctgat cgagtgtttt ggaaaaattg tggtagcctt cgtatccggg ttttgcgcac
      181 atgtagatgt aatcgttttt ctcataattc aacaccgcat caatggatgt aatatccgga
      241 acctgaatcg gaccgggtgg taatccggca ttcagatagg tgttatatgg ggatgcaact
      301 tcaagatgtt tgtttaaaac gcgacgcaga ttgaaatcgc ccactgcata aacaacagtg
      361 gggtccgact gcagacgtat tcctttcttc agacggttga gatacaatcc tgcaatgcgt
      421 ggtcgctctg aggcatgacg ggtttgctca gcctgcacaa tggatgccag tgtggaaacc
      481 tccgattggg aaagtccaag tgcttttgct ttcgacttac gttcttcggt ccagaagttc
      541 cggtaattgg atgcaataac cgataaaaat tcaccggcat cggtggccca gtccattttg
      601 taggtattgg gaataaacaa cgaaataaaa ttattgcggt tgaatccata acgcgcaatc
      661 gtgtctttgt tttgcagtac atcaatgatg gaaacggaat cggcctcgat gtatttgctc
      721 acttttccgg caatttgttc cagggttcgc acattgttaa acgttacttc tacctcaagt
      781 cgtccattac cggcacgcag gtgattaatc agatcattat tgctccaacc attgacgatt
      841 tcgtatttac ccggtacaat attctttccg cgtaaatttt tcttttcggc tacccagcga
      901 aatgttgccg tatcttcaag aatggattca ctcatcagta ttccggttaa atcatccaga
      961 tcggtaccgg tgggaatata aatgaatgtt ttttcttgct ttagctgaac attttttcca
     1021 tacatgcgcg aatacagcat gtttccgtag taacctccac ctataaggag gccaactacc
     1081 ataattacga ttatccgttt cattttcatt tataattcga gttgaagaaa atattcatcg
     1141 ctccatccac ttgaagtgcg aaaccaattt tcttttttac catttatttt aaatccacaa
     1201 cgttcaaaga gttgaaccga tgcggtgttt tcgcagagta tattgcaata aatgctgcgt
     1261 aataataaaa ttgaacggca atgctgaatt aattccataa gtgcttcatg agcatatcct
     1321 tttcctcgtt caccgtattc tccaatcaga ataccaactc ctgctcgttg atgaaccgca
     1381 tcaacatcaa acagatcgat aaatcctact gccgcattgt tctccgttaa ctcaattaca
     1441 aaacggaatt gtttgtcgcg gtaaatgtct ttttctgctt ccacatattc cttcaaaaga
     1501 tgtcggctaa acggaatata ggtactggtg accggccagt tttttgggtc gttttcccag
     1561 cggaccacaa tatcgaggtc gcccggttcc agtgcacgta aacggatgcg gggagtgctc
     1621 atgcagaagg tttgttttta agttcgcgaa tgcattgatc gatatccgac cggg
//
LOCUS       DAOMYG010000552         1663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_29062_length_1663_cov_9.485075, whole genome shotgun sequence.
ACCESSION   DAOMYG010000552 DAOMYG010000000
VERSION     DAOMYG010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29062_length_1663_cov_9.485075"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1523
                     /locus_tag="PK610_13645"
     CDS             <1..1523
                     /locus_tag="PK610_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009391773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRJ37034.1"
                     /translation="AVVGIQATIVTIEVNILPGGVYFHMVGLPDNAVKESQQRIRAAL
                     KNLDKRIPHGKEITVNLAPADIRKEGSAYDLSIAIGILAASEQIPSERVSEYIMLGEL
                     SLDGGFQPVKGVLPIAIAAKENGFKGVILPKANAREAAIVGEIEVLGVENITEVIEFF
                     SGDREIASTTVNIHEEFYERLDHYEFDFSDVKGQINIKRAFEIAAAGGHNIILIGPPG
                     AGKTMLAKRLCTILPPLSIEESLETTKIHSVAGKLSRNDALVTVRPFRSPHHTVSDVA
                     LVGGGGFPQPGEISLAHNGVLFLDELPEYKRTVLEVMRQPLEDRKVSIARARFSVEFP
                     ASFMLVAAMNPCPCGYYNHPEKECVCGPGIVQKYLNKISGPLLDRIDIHIEVTPVSYD
                     ELAAAAKTEGSKPIRERVIQARVVQEERFRMNPGLHCNAQMSSRMLREVCRIEKPSEQ
                     LMRNAMDKLGLSARAYDRILKVGRTIADLEGAEHIQTHHIAEAIQYRSLDRDNWGA"
BASE COUNT          492 a          372 c          396 g          403 t
ORIGIN      
        1 gtgccgtagt gggcatccag gcgactattg tcacaatcga agtaaacatt cttcccggag
       61 gggtttattt tcatatggta ggattaccgg ataatgcggt aaaggaaagc cagcaacgca
      121 tccgtgctgc attaaaaaac ctcgataaac gtattcccca cggaaaagag atcaccgtta
      181 atcttgctcc tgcggatatc cgtaaagaag gttcggcgta tgatctgtcg attgccattg
      241 gaatacttgc agcatccgaa cagatcccct ctgaacgtgt ttcggaatac atcatgctcg
      301 gtgaactttc gcttgatggt gggtttcaac ctgtaaaagg cgtgcttccc attgcgattg
      361 ctgccaaaga aaatggattt aaaggagtga tcttacccaa ggcgaatgca cgtgaagcag
      421 ccatagtagg tgaaatagaa gttttaggag tagaaaacat tactgaagtc atcgagttct
      481 tcagtggtga tcgcgaaatc gcatccacta ctgtaaatat tcatgaagaa ttttacgaac
      541 gactcgacca ttacgaattc gatttttcgg atgtgaaagg tcagatcaat attaaacgtg
      601 cctttgaaat cgcagctgca ggcggacaca acatcatcct tatcggacca cccggtgccg
      661 gaaaaacgat gttggcaaaa cgactgtgta caattcttcc cccattgagc attgaagaat
      721 cgctggaaac aaccaaaata cattcggtgg caggtaaact ttcgagaaac gatgccttgg
      781 taacggtgcg gccttttcgt tcaccacatc atacggtttc ggatgtagcg ctcgttggcg
      841 gaggaggttt tccacaaccg ggcgaaattt ctcttgcgca caacggagtg ctatttctgg
      901 atgagctgcc cgaatacaaa cgaacggtac ttgaagtaat gcgccaaccg cttgaagaca
      961 gaaaagtgtc cattgcacgg gcacgtttta gtgtggaatt ccctgcgagt tttatgttgg
     1021 ttgcagcgat gaatccttgt ccatgcggtt actacaacca tcccgaaaaa gaatgtgtct
     1081 gcggaccggg tatcgtgcag aaatacctca acaaaatcag cggacctcta ctcgaccgca
     1141 tcgacattca tattgaggtg acacccgttt cgtacgatga attagcagca gctgcaaaaa
     1201 cggaaggaag caaacccatt cgtgaacgtg taattcaggc gcgcgttgta caggaagaac
     1261 ggttccggat gaatcccggt ttacattgca atgcacaaat gtcgtcacgc atgctacgcg
     1321 aagtttgtcg catagaaaaa ccaagcgaac aattgatgcg caatgcgatg gataaactcg
     1381 gattatctgc cagagcctac gaccgtattc ttaaagtagg aagaaccatt gccgaccttg
     1441 aaggtgctga acacatccaa acccatcaca tcgctgaggc aatccaatac agaagtctgg
     1501 atcgcgataa ctggggagcc tgaaacgcta ttgaagaaaa gataacaatt atttcttcgg
     1561 cagcgcattc aaacgctcat aggttgcgaa atattcattc gctttaatgg aatcacccaa
     1621 tgaacgataa gtaaatccga gaaagcgata ggcattggga tat
//
LOCUS       DAOMYG010000553         1660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_29133_length_1660_cov_7.150156, whole genome shotgun sequence.
ACCESSION   DAOMYG010000553 DAOMYG010000000
VERSION     DAOMYG010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29133_length_1660_cov_7.150156"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PK610_13650"
     CDS             complement(<1..190)
                     /locus_tag="PK610_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ37035.1"
                     /translation="MQLARLSLFSFKNISDLSLEFCPGVNCLLGDNGEGKTNILDAIH
                     YLSFCKSYFNLSDIQNIKH"
     gene            293..988
                     /locus_tag="PK610_13655"
     CDS             293..988
                     /locus_tag="PK610_13655"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ37036.1"
                     /translation="MSEQNTSENNSFFKKAERYVRENQRSLIIIGGVALTLVLGFFAY
                     LKFYKGPKEVEAQDAMWKAQYYFEIDSFNLAINGTIDPKTGESYAGFLQIADEYSGTQ
                     AGNLANYYLGICYLNLGQYQAAIDALEQVDLSDQIIQPMAIGALGDAYMELGDIENAI
                     SNYESAAKASSNQFTSPLYLKKAALALEETGDYKKALDHYEKILKDYEGSDEGRDIEK
                     YIARAKGRAGITE"
     gene            988..1476
                     /gene="ribH"
                     /locus_tag="PK610_13660"
     CDS             988..1476
                     /gene="ribH"
                     /locus_tag="PK610_13660"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRJ37037.1"
                     /translation="MATSGKDLSSYNPNDVPNGTAFSIAIVRAEWNHDITSALYKGAF
                     DTLMDNGVKESNITSIHVPGAFELPVAAKWIIEQKKPDAVIVIGCVIQGETKHFDFVC
                     NGVTQGVMDLNLSTGIPVIFCVLTDNTHQQSVDRSGGKHGNKGIECAVAALKMAAIRK
                     EI"
BASE COUNT          508 a          326 c          367 g          459 t
ORIGIN      
        1 catgtttaat gttctgaata tcagataaat taaagtagct tttacaaaat gagaggtaat
       61 gaattgcgtc cagaatattg gttttcccct ccccgttatc acccaaaaga caattcacac
      121 ccgggcaaaa ctccaggctg agatcgctga tatttttaaa gctaaaaagg gaaagtcggg
      181 caagttgcat gtgctgcaaa agtagctatt tagcccccat tcgctcaaga gattgacaaa
      241 ggacttcgtt tgggttgaaa aaaagttatt tttgcactcc aattcattgt ccatgtcaga
      301 acagaacaca tccgaaaaca attccttttt caaaaaggca gagcgctatg tacgtgaaaa
      361 tcagagaagt ctgatcatta tcggcggagt tgcgcttacc cttgtgctgg gtttctttgc
      421 gtacctgaag ttttataaag gtccaaaaga agtagaagcg caggatgcaa tgtggaaggc
      481 acaatactat ttcgagattg attctttcaa tctggcgatt aatggaacga tcgatcctaa
      541 aaccggtgaa agttatgcag gatttctgca aatcgccgat gagtacagcg gaacacaagc
      601 cggaaatttg gccaactatt accttggtat ttgttatttg aacctgggcc agtaccaggc
      661 tgcgattgat gctttggaac aagttgattt gagtgatcag atcattcaac caatggccat
      721 cggtgcatta ggagatgcgt atatggagtt gggcgatatt gaaaatgcca tttccaatta
      781 cgaatccgct gcaaaagcat cttcgaatca attcacttca cccctttatc tgaagaaagc
      841 tgctttggcg cttgaagaaa ccggtgatta taaaaaagca ctcgaccatt acgaaaagat
      901 ccttaaggat tacgaaggtt cagacgaagg ccgcgatatt gaaaaatata ttgcccgtgc
      961 taaaggtcgt gccggtatta cagaataatg gctacctcgg gtaaagatct ttcttcctac
     1021 aacccgaatg atgtccccaa cgggactgcg ttcagcattg cgatagtgcg tgcagagtgg
     1081 aatcatgata ttacttccgc actctataaa ggcgcattcg atacattaat ggacaatggc
     1141 gtaaaggaat cgaacattac ttccattcac gtacccggtg catttgaatt gccggtagct
     1201 gccaaatgga taattgaaca gaaaaagcca gatgcggtta ttgttattgg ttgtgtaatt
     1261 cagggtgaaa caaagcattt tgattttgtt tgcaatggtg ttacgcaagg agtaatggat
     1321 ctcaatcttt ccaccggaat tcccgtaatt ttttgtgtgt tgaccgacaa tacccatcag
     1381 caaagtgtgg atcgttcagg cggaaaacat ggcaataaag gcattgaatg cgcagttgcc
     1441 gctctgaaaa tggcagccat tcgaaaggaa atttaaaccg ttcgtcaaat ttccaagtac
     1501 cgaaatccga cttaaatacc tcataattaa ggaggcggga acatgcattc gtctatttat
     1561 ggttagtggt taaacagaat atgtctatct ttcggcatgg atttgattcg aaaggaaatt
     1621 taaaccgttc gtcaaatttc caagtaccga aatccgactt
//
LOCUS       DAOMYG010000554         1648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_29460_length_1648_cov_9.829253, whole genome shotgun sequence.
ACCESSION   DAOMYG010000554 DAOMYG010000000
VERSION     DAOMYG010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29460_length_1648_cov_9.829253"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="PK610_13665"
     CDS             <1..1199
                     /locus_tag="PK610_13665"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ37038.1"
                     /translation="QNVNEEIINSIRRDLGLDLPLYKQYLLYLNDLSPISLHSDNPDS
                     HIHWDEEKYSGSRLFSVGGDRLLSIKLPHFRRSYRSKQKVADIIMESLPGTIVLALSA
                     MVFATMIGIFLGIFSALNKGSFFDSFALVVAVLGMSGPSHFMAIIIAWIGGFLWYETT
                     WLPQLPMYFMLLGLLVGAAFNRYHTKNKISRKFSYQFLFESGLKGFGVGVLGWALGLG
                     INGIMGYEFLPLIDAYFPLPGTELETTGSLYEVDNLTGEEYLKLSNLILPAFTLGIRP
                     LAIVLQLTRSSMLDVMSQDYIRTATAKGLSKYTVVVKHSLKNALNPVITAISGWFASL
                     LAGAVFVENIFNWNGIGKKVFDSVVNDDFPVVVGSVLIIATMFVVINILVDIIYGILD
                     PRIRIR"
     gene            1196..>1648
                     /locus_tag="PK610_13670"
     CDS             1196..>1648
                     /locus_tag="PK610_13670"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRJ37039.1"
                     /translation="MMKRKKIVAGNWKMNHSYHEALAVFAQLLEHKEEFPANAEVIIC
                     PPALYLREFAKKAGNHVALGAQNCHFEVEGAFTGEISAEMIKSVGARYCILGHSERRS
                     MFAETDEIVSRKLRHALTVSLNVILCCGESLEVREDDNHFAWVQQQLNI"
BASE COUNT          407 a          340 c          388 g          513 t
ORIGIN      
        1 gacagaatgt aaatgaagaa atcatcaaca gtatccgtcg tgacctgggg cttgatttgc
       61 cattgtacaa acaatacctg ctttacctga atgatctttc gccgatctcc ctgcatagcg
      121 ataatcctga ttcgcatatt cattgggacg aagagaaata ttccggttca cgtttgtttt
      181 ctgttggtgg agatcgtttg ttatccatta aactgccgca tttccgtcgc agttaccgtt
      241 ccaaacaaaa ggttgccgat atcatcatgg aatcgcttcc gggtaccatc gtgcttgcac
      301 tttctgcaat ggtgttcgca acgatgattg gtattttcct cggcattttt tctgcgctca
      361 ataagggaag tttcttcgat agttttgcac ttgttgttgc tgtgctgggt atgtccggtc
      421 cttcgcattt tatggctatt atcattgcat ggatcggagg ttttctctgg tatgagacta
      481 cctggttgcc gcaattgcct atgtatttta tgttgctggg cttactggtt ggtgctgcgt
      541 ttaaccgcta tcataccaag aataaaatat cccgaaagtt ctcgtaccag tttcttttcg
      601 aatccggatt aaaaggtttt ggagttggtg tattgggatg ggcactgggc ctgggtatta
      661 acggcattat gggatacgaa ttccttccgc ttatcgatgc ttattttccg cttcccggaa
      721 ctgaactgga aacaacagga tcgctctatg aagtggataa tctcacaggt gaagaatacc
      781 tcaaactaag caatcttatc ctcccggctt tcacattggg aattcgtcct ttggctatcg
      841 tacttcagct tacacgcagt tcaatgctgg atgtgatgtc gcaggattat atccgcacag
      901 caacggcaaa aggtttatcg aaatacacag tggtggttaa acattcgctc aaaaatgctt
      961 tgaatcctgt gattactgcc atctccggat ggtttgcttc tttgcttgca ggggctgtct
     1021 ttgtggagaa tattttcaac tggaacggaa tcggaaaaaa ggtattcgat tcggtggtga
     1081 acgatgattt tcctgtagtg gtaggtagcg ttttaattat tgcaaccatg tttgtggtga
     1141 tcaatattct ggtcgatatc atttacggta tcctcgatcc acgcatccga ataagatgat
     1201 gaaaagaaaa aaaattgttg caggtaactg gaaaatgaac cacagttacc acgaagctct
     1261 tgctgttttt gcgcaattgc tcgaacacaa ggaagaattt cctgccaatg cagaagtgat
     1321 catttgtccg cctgcattgt acctccgcga attcgctaaa aaagcgggta accatgtcgc
     1381 gctgggtgca cagaattgtc acttcgaagt tgaaggcgca tttacaggtg agatttcagc
     1441 tgaaatgatc aagtcggtgg gtgcacgtta ttgcattctc ggtcattccg aacggcgatc
     1501 gatgtttgct gaaactgatg aaattgtttc ccgcaaattg cgccatgcgc ttaccgtttc
     1561 gctgaatgtg attttatgct gcggagaatc gctcgaagtg cgtgaggatg ataatcattt
     1621 tgcctgggta cagcaacaac tgaacatc
//
LOCUS       DAOMYG010000555         1643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_29595_length_1643_cov_11.704660, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000555 DAOMYG010000000
VERSION     DAOMYG010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29595_length_1643_cov_11.704660"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PK610_13675"
     CDS             <1..392
                     /locus_tag="PK610_13675"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRJ37040.1"
                     /translation="GVQMQVPIFSSGMRNAKVAQTRIEMEKSQINLQQVDQALRIQSY
                     MAQTEFLTALDAYNYQKESLALAESIQSKTLLKYKQGLASSLELSQAQNQYLNQQTNY
                     ITSLFNLLSAKAQLDRINSKSAGAITK"
     gene            420..1490
                     /locus_tag="PK610_13680"
     CDS             420..1490
                     /locus_tag="PK610_13680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRJ37041.1"
                     /translation="MKPFHFTIAALLVVLQSCSVEVAEETTVTEPVQVLPIVTTKKID
                     YSQFQHYVEVQGTIQADKIATLTAEMGGLIRVIHVEKGQRVNAGQTLVTLDSDVLRKN
                     IEEVKKSLELADFVYEKQKNLHEQNVGSELQYQQAKNNKERLEQTLKTLESQLSMTVI
                     SAPFDGVIDDIFPKIGEMAAPGVPVIRLINLRKVHIEAEVMESYLRSVTKSKMVDVRI
                     NSMDTMIYNVPIVRTGKYINPANRTFVVQVDINNTDERLLPNMIAILRIKDEVMDSVL
                     AVPNEALLQSSAGVNYLFVVYEENGKELVRKIDVEPGASDDTRTVVYPLNGARSQLRA
                     GDRIVVEGARGVKQGMQVKVKN"
BASE COUNT          526 a          315 c          379 g          423 t
ORIGIN      
        1 ggggagtaca aatgcaggtt ccgattttca gttcaggtat gcgcaatgcg aaagttgcac
       61 aaacgcgcat tgaaatggag aagtcacaaa tcaatcttca gcaagtcgac caggctttgc
      121 gtattcagtc gtacatggcg caaacagaat tcttaacggc gttggatgcg tacaattatc
      181 aaaaggaatc gctggcactg gccgagagta ttcaatcgaa aaccctactg aagtacaagc
      241 agggattagc atcgagcctc gaactttcgc aggcgcagaa tcagtattta aatcaacaga
      301 ccaattatat cacttcattg ttcaatcttt taagtgcaaa agcacaactc gaccgaatca
      361 actcaaaatc ggccggagcc atcactaaat aaatgaacct accgaaacca gatatcatca
      421 tgaaaccatt tcactttact atcgctgctt tgcttgttgt tttgcaatcc tgcagtgttg
      481 aagtcgccga agaaacaacc gtaaccgagc ctgtacaggt tttgccaatt gtaaccacta
      541 aaaaaatcga ctattcccaa tttcaacatt atgttgaggt acaaggaacc attcaagccg
      601 acaagatcgc aaccctgacg gcggagatgg gcggattgat tcgtgtaatt catgttgaga
      661 aaggtcaacg tgtgaatgcc ggacaaacgc ttgttacgct cgatagtgat gtattacgca
      721 agaacataga agaggtgaag aagagtcttg agctagcaga ttttgtatat gagaagcaaa
      781 agaatctgca tgagcagaat gtgggatcgg aattacaata ccaacaagca aagaacaata
      841 aggagcgatt ggagcaaact ttaaaaacgc tggaatctca attgagcatg acggtaattt
      901 cagctccttt tgatggagtg atcgatgaca tttttccaaa aattggtgag atggcagctc
      961 cgggggttcc ggtaattcgt ttgattaatt tgcgcaaagt gcatattgaa gcggaggtga
     1021 tggaaagtta tttacgcagt gtaaccaaaa gcaaaatggt tgatgtacgg atcaactcca
     1081 tggataccat gatttacaac gtgcctatcg ttcgcacagg aaaatatatc aatcccgcaa
     1141 accgcacatt tgttgttcag gtggacatca ataatacaga tgaacgtctt ttacccaata
     1201 tgattgctat actgcgaata aaggatgaag tgatggatag tgtgttggcg gtacccaacg
     1261 aagcattgtt acagagttct gcaggagtaa attatttgtt tgtagtctat gaagaaaacg
     1321 gaaaagaact tgtcaggaaa attgacgtgg agccaggcgc ttcagatgat acccgcactg
     1381 ttgtttatcc cttgaatgga gcgcgctcac agctacgtgc aggcgatcga attgttgtag
     1441 aaggagcccg tggcgtgaag cagggtatgc aggtaaaagt gaagaattaa tcattaaaaa
     1501 aattcagttt tatgtcgacc ccaaaaaagg attccgataa acgattattg ctcaaggatt
     1561 ttttcctgac gaattgggcc atcagcaatg taaaaacagt attgctgatt acgttcatga
     1621 ttgtttttgc aggtatcagc act
//
LOCUS       DAOMYG010000556         1626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_30040_length_1626_cov_6.240611, whole genome shotgun sequence.
ACCESSION   DAOMYG010000556 DAOMYG010000000
VERSION     DAOMYG010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30040_length_1626_cov_6.240611"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..>1626
                     /locus_tag="PK610_13685"
     CDS             77..>1626
                     /locus_tag="PK610_13685"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRJ37042.1"
                     /translation="MRFCFLFILSVLIPVCSVSAQRSGVLKGRVVDELGKPLKDVNIY
                     VTPLHTGTTTNEDGTYLLILPSDSSLSVSYSYSGHERKTILVRLKKGEEKTQNVLLQF
                     QTLIEITVDGGVKRPPIEKMPKIEFKQLPTMTGNVEDLIKSFGMGVSSNNEMSAQYSV
                     RGGNFDENLIYVNDIEIYRPFLVRSGQQEGLSFINSDMVEDIYFSSGGFEARYGDKMS
                     SVLDIKYKSPIEKKGSASMSLLGGSVHFEGKVKQRFSYITAFRYRSNSYVLNALPTKG
                     DYRPVFADGQFLLTYKARENWNLSFLGHYSNNLYRMIPETRETDFGTVNEALRLTVFF
                     DGQEITRFETMTGALTSEHILSEKTTIKFIGSVYRSIESESFDIQGQYRLSELERDLG
                     SENFGEVLFVRGVGTFLNHARNRLDAFVYNVSHLGQHELKNGAKINWGLRYQGERIND
                     KLSEWNMLDSAGFSIPQIPSDQIILNDVIKAKIRLSSSRYTGHLQYSQSIDRKKEIKL
                     GDSTFTSI"
BASE COUNT          477 a          346 c          345 g          458 t
ORIGIN      
        1 ttatcttcaa aaaagcgatc aacacaatat ctcgcctatt ccccgtttta gagtggtttt
       61 gctacttttg acctctatgc gattttgctt tctattcatc ctttccgttt tgatcccggt
      121 atgcagtgtt tccgcacagc gctccggtgt attgaaaggc cgtgttgtgg atgaattagg
      181 aaagccactc aaagacgtca acatttacgt tacgccgctg catacgggta ccacaacaaa
      241 tgaagatgga acctatctgc tcattctgcc ttctgactct tcgctttctg tatcttattc
      301 atattccggt catgaacgaa aaacaattct tgttcggcta aaaaagggtg aagaaaaaac
      361 acaaaacgta ctgcttcaat ttcaaaccct catcgagata accgttgatg gaggtgtgaa
      421 acgtcccccg attgaaaaaa tgcccaagat cgagtttaaa caacttccaa caatgacagg
      481 gaatgtggaa gatcttatca aatcatttgg gatgggagtt tcttccaata atgagatgag
      541 tgcacaatat tcggtgcgcg gcggaaattt tgatgagaat ttaatttatg taaacgacat
      601 tgaaatctat cgtccttttc tcgtgcgaag tggtcaacag gaaggattat cattcatcaa
      661 ttcagatatg gttgaagata tctacttctc atccggaggg tttgaagccc ggtatggcga
      721 taagatgtcg tcggtattgg atatcaaata caaatcgccc atcgaaaaaa aaggatctgc
      781 atccatgagt ttgctcggtg gttccgtgca tttcgaaggt aaggtcaagc aacgtttctc
      841 ctacattacc gcattccgtt accgttcgaa ttcctatgtt ctgaatgcgc ttcctacaaa
      901 aggtgattat cgtccggtat ttgccgacgg acaatttcta ctcacctaca aggcaaggga
      961 gaattggaat ctttcctttt tgggacatta ttccaacaac ctctaccgca tgattccgga
     1021 aacaagagaa acagatttcg gaacggtaaa cgaagccttg cgtcttactg tatttttcga
     1081 tggccaggag ataacacgat tcgaaacgat gaccggcgct ttaacatcgg agcacattct
     1141 ctctgaaaaa accaccatta aattcattgg ttccgtgtac cgcagtattg aatcagaatc
     1201 cttcgatata cagggacaat accgactttc ggaattggag cgtgatcttg gatcagaaaa
     1261 ttttggtgaa gttttatttg tccgtggggt cggaacattt ctgaatcatg cccgcaacag
     1321 gttggatgcc tttgtttaca atgtttcgca tttaggtcag cacgaactta aaaacggtgc
     1381 aaaaattaat tggggattac gctatcaggg agaacggatc aacgacaagc taagcgaatg
     1441 gaacatgttg gattcggcag gattttcaat tccgcaaata ccaagtgatc agatcatcct
     1501 caacgatgtc atcaaggcga agatccgttt atcctccagc agatataccg gacatttgca
     1561 atacagtcaa tcgatcgacc gaaaaaaaga aattaagttg ggagacagta ccttcaccag
     1621 catcag
//
LOCUS       DAOMYG010000557         1621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_30158_length_1621_cov_9.420817, whole genome shotgun sequence.
ACCESSION   DAOMYG010000557 DAOMYG010000000
VERSION     DAOMYG010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30158_length_1621_cov_9.420817"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1604)
                     /locus_tag="PK610_13690"
     CDS             complement(<1..1604)
                     /locus_tag="PK610_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37043.1"
                     /translation="MKRFFYSVSFLLASLASMAQVTGPSSSQSPYCAPTFPGASYTSL
                     FTVGDQVGTYRMVGIPDGMGAYDNGDGTFTILLNHELGNTNGIARAHGNAGAFVSKWI
                     VNKATLTVISGADLIQEVYLWNGSTYTNHNSGNPMSTGFNRFCSADLPEQSAFFNSQS
                     GKGTSEKIFMNGEEGGTEARGFAHIVSGPNAGKSYELPHLGKYSYENALASPYMQDKT
                     IVIGLDDATPGEVYVYIGNKSNTGNEIEKAGLVGGTLYGVAVSGLSTEVSGSFIAPNT
                     AFSLANLGLVHNMTGATLQTNSSALSVTKFLRPEDGAWDLRNPNDFYFVTTNAFNSPS
                     RMYRLRFADITQPELGGTITAVLDGTEGTQMMDNIGMDYYGNIVIQEDPGGQAYLAKT
                     WNYYVDSDVLTELASHDATRFVTGGANFLTQDEESSGIIDVQGILGAGYYVGCTQAHY
                     NIAGELVQGGQMYVLYNPFTYNANPEISVTGNTINIVDGDNTPSATDYTVLGNVSLGA
                     NKTRSFVINNAGPGPLKVTGMNISGA"
BASE COUNT          466 a          356 c          310 g          489 t
ORIGIN      
        1 tgagcccctg aaatattcat tccggtaact tttaacggtc caggtcctgc attgttgatc
       61 acaaatgaac gggttttgtt tgcaccaaga gaaacatttc ctagaacagt ataatcagtt
      121 gcagaaggtg tattatcacc atctacaata ttaattgtat ttccggttac tgaaatttca
      181 ggattggcgt tgtatgtaaa aggattgtaa agcacataca tctgtccgcc ttgaacaagc
      241 tcgcctgcga tattataatg cgcctgagtg caacctacat aataaccagc accaagtatt
      301 ccctgcacat caattatacc ggatgattct tcatcctgag taaggaagtt tgctccacct
      361 gttacaaaac gtgttgcgtc atgtgatgca agttcagtaa gaacatccga atctacatag
      421 taattccagg tttttgcaag gtaggcttgt cctcccggat cttcttgtat tacaatatta
      481 ccgtaataat ccattccgat attatccatc atctgagtgc cttcggttcc atcaagaact
      541 gcagtaattg tacctcccaa ttcaggttgt gtaatatctg caaatcgcag acggtacatg
      601 cgcgaaggac tgttgaatgc gttcgttgtt acaaaataga aatcattcgg attacgaaga
      661 tcccaggcac catcttccgg tcttaggaat ttagtaacgc ttagtgcaga actattggtt
      721 tgcaacgttg cgcctgtcat gttatgcaca agaccgaggt ttgcgagtga gaatgcagta
      781 tttggagcaa taaaacttcc acttacttca gttgagagtc ctgatactgc aacaccatac
      841 aatgttcctc caaccaaacc ggctttttct atttcatttc ctgtatttga tttattacca
      901 atgtaaacat aaacctctcc gggagtggca tcatccaatc caattacaat tgttttatcc
      961 tgcatgtaag gagatgcaag tgcattttcg tatgaatatt ttccaaggtg tggaagctca
     1021 tatgattttc ctgcattggg tccagaaacg atgtgagcaa accctctcgc ttctgttcct
     1081 ccttcttcac cgttcataaa gattttctcg ctcgtacctt ttccactttg cgaattgaag
     1141 aatgcacttt gctcaggcaa atctgcagag cagaaacgat taaatccggt ggacattgga
     1201 ttgcctgaat tgtgattcgt ataggtagat ccattccata agtaaacctc ttgaataaga
     1261 tctgctccag aaataacagt taaggttgct ttgtttacaa tccatttgga tacaaaagct
     1321 cccgcattac catgagcacg ggcaatacca tttgtattcc ctaattcatg attaagtaag
     1381 atggtgaaag ttccatctcc attatcatag gctcccattc catcaggaat tccaaccata
     1441 cgatatgtac ctacctggtc accaacagtg aacaaagagg tataacttgc acccggaaat
     1501 gtcggagcac aataaggcga ctgcgaactt gaaggccctg tcacctgagc catacttgcc
     1561 agtgaggcta gcaagaatga aactgaataa aaaaatcttt tcattttatt tgggttttag
     1621 t
//
LOCUS       DAOMYG010000558         1598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_30881_length_1598_cov_9.755023, whole genome shotgun sequence.
ACCESSION   DAOMYG010000558 DAOMYG010000000
VERSION     DAOMYG010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30881_length_1598_cov_9.755023"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1598
                     /gene="dnaE"
                     /locus_tag="PK610_13695"
     CDS             <1..>1598
                     /gene="dnaE"
                     /locus_tag="PK610_13695"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRJ37044.1"
                     /translation="IKPIVGCEFNVCRNHLDKSVKDNGSHVVLLAKNEAGYKNLAKMS
                     SIAFVDGFYYVPRVDKSIILNYKEHLIATTGGIFGEVPNLILNVGEEQAEEAFIWWKE
                     QFGDDFYAELMRHGQDEEEKVNQVLLRFCEKHGVKYFASNNTFYLDQTDAKAHDILLC
                     VKDGEKADTPIGKGRGFRYGFPNDQYYFKSQDEMKALFADLPDAIITTNEIVDKVEPF
                     KLERNVLLPKFEIPDEFRHPEDETDGGKRGENAFLRHLTYEGAKKRYSEITPEIQERL
                     DFELATIENTGYPGYFLIVQDFTTEARNMGVSVGPGRGSAAGSAVAYCIGITNVDPIK
                     YDLLFERFLNPERVSLPDIDIDFDDEGRDKVIQYVVNKYGASQVAQIITYGTMAAKSA
                     IRDTARVLDLPLADADRIAKLVPDLTLAKLLELKDDELKEKLSGEDLEMGLQLRKIAK
                     ADDISGQTLRQARMLEGSLRNTGIHACGVIITPEDITHIVPVATAKDSELYVTQFDNS
                     IIESAGLLKMDFLGLKTLTIIKNA"
BASE COUNT          493 a          293 c          375 g          437 t
ORIGIN      
        1 cattaaaccc attgtgggtt gtgaattcaa tgtttgcaga aaccatttgg ataaatcggt
       61 aaaagataat ggaagtcatg tcgtgcttct ggcaaaaaac gaagcagggt ataagaatct
      121 tgcaaagatg tcgtcgattg cgtttgtaga cggattctat tacgtaccga gagtagacaa
      181 atcgataatt cttaactaca aggaacattt aattgcgacc acaggaggaa tttttggaga
      241 agtacccaat ctcatattga atgttggaga agaacaggct gaggaagcct ttatttggtg
      301 gaaagagcaa ttcggggatg atttttatgc cgagttgatg cgtcatggtc aggatgaaga
      361 agaaaaagta aaccaggtac tcttacgctt ttgcgaaaag cacggtgtaa aatattttgc
      421 atcaaacaat accttttatc tcgaccagac cgatgccaaa gcgcacgata ttttgctctg
      481 tgtaaaagat ggtgaaaaag ctgatactcc cattggaaaa ggacgtggat tccgttatgg
      541 atttccgaac gatcagtatt attttaaaag tcaggatgaa atgaaggcat tgtttgccga
      601 tttacctgat gcgattatta caacaaatga aattgttgac aaggtagaac ccttcaaact
      661 ggaacgaaat gtattgctgc caaagtttga aattcccgat gaatttcgtc atcccgaaga
      721 tgaaacagac ggtggaaaaa ggggtgagaa cgctttttta cgtcacctta cctacgaagg
      781 agctaaaaaa cgttacagtg agatcactcc tgaaattcag gaaaggctgg attttgaatt
      841 agccacaatt gaaaatacag gatatccggg ttactttctg attgtgcagg attttaccac
      901 agaagcacgc aatatgggtg tgagtgttgg tcctggtcgt ggttctgctg ctggatctgc
      961 agttgcgtat tgtattggaa tcaccaatgt tgatcccatt aagtacgatc tcctttttga
     1021 gcgttttctc aatccggagc gggtttcact ccccgatatc gatatcgact ttgatgatga
     1081 gggtcgtgat aaagtcattc agtatgtggt aaacaaatat ggcgcctcgc aggtagctca
     1141 gatcattacc tacggaacca tggctgcaaa atctgcaata cgcgatacag ccagggtact
     1201 tgatttaccg ctagccgatg ccgaccgtat agcaaagctt gtacctgatc ttacgttggc
     1261 aaaactgttg gaattaaaag acgatgagct aaaggaaaaa ttatccggtg aagaccttga
     1321 gatgggtctt caactgagaa aaatcgccaa agcagatgat atttccgggc aaaccttgcg
     1381 tcaggcacga atgctggagg gctcactcag gaataccggt attcatgcct gtggtgtaat
     1441 tataactccg gaagacatca cccacattgt tccggtggca acagcaaagg attcggaatt
     1501 gtatgttacg caatttgaca actctattat tgaaagtgca ggtttgttga aaatggactt
     1561 tttgggatta aaaaccctaa ccatcataaa aaatgcct
//
LOCUS       DAOMYG010000559         1596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_30952_length_1596_cov_5.277093, whole genome shotgun sequence.
ACCESSION   DAOMYG010000559 DAOMYG010000000
VERSION     DAOMYG010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30952_length_1596_cov_5.277093"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..>1596)
                     /locus_tag="PK610_13700"
     CDS             complement(172..>1596)
                     /locus_tag="PK610_13700"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRJ37045.1"
                     /translation="YGSIVLRHSEYQVNREFPEKFFGPEIRRYEEQAYDRDSLYWVKE
                     RTFPLTDSEQQFAHNCDSLRDYYKSDEYYRKRDSSFNKINIWSFLVNGVGHRDRVKGY
                     EFFINPLVVQMIPFGIGGYRHRLGGYYNKRLENGMLLETTGDIDYGFANKDIKGKGGV
                     GLTYNPKKFTRTFIRFGDYYDMINNYASVSTFLSRSNFARNKTFSIAQRTEIINGLYG
                     ELTFEYSDQQAISNLKMDSWSQDVFDSLNTPTAFDRYTKSEFKLQLNYKIRQPYTIRK
                     GRKMVYASPYPELHLVYRKGMPGLFGSEVNFDYIELGAKNQRKLARFGDLKWNIQAGS
                     FFNRNSLRLLEYKFFRGSDPFLFSDPLKSFQLMGPTLSTSTAFLRANIIHHFDGILLS
                     KIPVINWLKISLAAGGGTLLIPQEDFAHIELFGGVERVFRIRKQLIRFGAYAVTSDNT
                     FENADFTFKFGISFFNPFTGRYDY"
BASE COUNT          465 a          356 c          313 g          462 t
ORIGIN      
        1 gataaagagg ttctgtgcat ttcctccact tcattcacat caattacggc tccgtcgggc
       61 acaataaaac agatggtatc cttatcaatc agatgtgcat gaaaggatgg ttgctctgtt
      121 ttggggaccg gcattcttat tggtttgggt tataataatc cgaaaaaatt ctcaataatc
      181 ataacgcccc gtaaagggat tgaagaaact gattccgaac ttgaaggtaa aatctgcatt
      241 ttcgaatgtg ttatccgaag taacggcata ggcgccaaaa cgaatcaatt gcttacgtat
      301 tcgaaaaacc cgctctactc caccaaataa ttctatatgc gcaaaatctt cctggggaat
      361 taacaaggtt cctcctcctg ctgccaatga aatcttgagc cagttgatta ccggaatttt
      421 actcaataga atcccgtcga aatgatgaat aatattcgct cgcaaaaagg cggttgaagt
      481 acttaatgta ggtcccatca actgaaatga ttttaaaggg tccgaaaaca ggaatgggtc
      541 gcttccacgg aaaaatttat actccagcaa gcgaagactg tttctattga aaaaagaacc
      601 tgcctgtata ttccatttca gatcgccaaa acgtgccagt ttacgctgat tttttgcgcc
      661 cagttcgata tagtcgaagt tcacctcact gccaaacaac ccgggcatcc ctttcctgta
      721 aaccagatgc aattcaggat agggtgaagc atacaccatt tttcttcctt tacgaatggt
      781 ataaggttga cggattttat aattcaactg caacttgaat tcacttttgg tataacgatc
      841 gaaggccgtt ggcgtattga gtgaatcaaa aacatcctgc gaccagctgt ccatttttaa
      901 attcgaaatt gcctgctgat cgctgtattc aaatgtaagt tcgccgtaca aaccattaat
      961 gatttctgtg cgctgagcaa tactgaatgt tttatttcgt gcaaagttgc tccggcttaa
     1021 aaaggtggat accgatgcat aattattgat catgtcgtag taatcgccaa agcgaatgaa
     1081 cgttcgggta aactttttcg gattataagt cagcccaact ccccctttcc ctttgatgtc
     1141 tttattggca aaaccataat caatatctcc ggttgtttcc aacagcattc cgttttcgag
     1201 acgtttgttg tagtaaccac caaggcggtg acggtatcct ccaatgccaa atggaatcat
     1261 ctgcaccacc agcggattaa taaaaaattc gtatcccttt accctatccc tatgtccgac
     1321 accattcacc aaaaacgacc agatattgat cttgttaaag gaagaatcgc gtttacggta
     1381 atactcatca ctcttgtaat agtcgcgcag tgaatcgcaa ttgtgcgcaa attgctgttc
     1441 cgaatctgtg agaggaaatg tccgttcttt cacccaatat agagaatcac gatcgtaagc
     1501 ctgctcttcg tagcgacgaa tttccggtcc gaaaaatttt tccgggaact cgcggttgac
     1561 ctggtattct gagtgccgta aaacgatact tccgta
//
LOCUS       DAOMYG010000560         1592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_31049_length_1592_cov_5.772284, whole genome shotgun sequence.
ACCESSION   DAOMYG010000560 DAOMYG010000000
VERSION     DAOMYG010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31049_length_1592_cov_5.772284"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PK610_13705"
     CDS             complement(<1..268)
                     /locus_tag="PK610_13705"
                     /inference="COORDINATES: protein motif:HMM:NF014898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRJ37046.1"
                     /translation="MQHSSNITVQAFAVFLKQSRFSAQTIRSYQYIVDKFLSENPNGC
                     NYSYSEILSYLESLNRYDIGKSTKQLKLTVLKKFFDFLIETEQRD"
     gene            complement(258..851)
                     /locus_tag="PK610_13710"
     CDS             complement(258..851)
                     /locus_tag="PK610_13710"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRJ37047.1"
                     /translation="MAKSVSRNVVTLAEIRLLFNACADKRERALLSIAYGCGLRRTEI
                     VRLNREDVDLTEGILTVREGKFGKTRTVPLSDFVLKDLREYVIQERPKLLEFGEGTQS
                     FFVNNQGNRMQGLAMLTYLKKIIDRTKDQKLKQKGITLHCLRHSIATHLLDNGAEMEF
                     VQRMLGHTMIDTTHIYSKRRKQKKIILEQFKNKVGAA"
     gene            complement(1306..>1592)
                     /locus_tag="PK610_13715"
     CDS             complement(1306..>1592)
                     /locus_tag="PK610_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010521197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37048.1"
                     /translation="KEKWQSASDFTQRDVRLALNVSKTQCFRYFEQLELLEYIQRTGG
                     YANRGFKYKIVYWDDMEKTREKIKCELQKQLVALGGSEGLEHQNPHVSKD"
BASE COUNT          416 a          381 c          286 g          509 t
ORIGIN      
        1 cgtctctctg ttcggtttca ataaggaaat cgaaaaattt cttcagcaca gtcagtttta
       61 gctgcttagt ggatttaccg atatcgtatc ggtttaaaga ctcaaggtaa ctcagtattt
      121 cactgtaaga ataattacac ccattgggat tttcactcag gaatttatct acgatatatt
      181 gataactcct gatcgtttga gccgaaaacc ggctttgctt taaaaaaacc gcaaatgcct
      241 gaaccgttat gttactacta tgctgcaccg actttgtttt taaattgctc cagtatgatt
      301 tttttctgct tcctgcgttt gctgtaaata tgcgtggtat ctatcatcgt atgaccaagc
      361 attcgttgta caaattccat ttcggctccg ttgtccaaca aatgcgtggc gatactatgg
      421 cgcaagcagt gaagcgtgat tcctttttgt ttcagttttt gatctttcgt ccggtcgata
      481 atttttttca ggtaagtaag catcgccaac ccctgcatcc ggtttccctg attatttaca
      541 aaaaacgatt gcgttccttc accgaattct aaaagtttcg gtcgctcctg aataacatat
      601 tcccgaagat ctttcaacac gaaatccgaa agcggaacgg tacgcgtttt cccgaatttc
      661 ccttcacgaa ccgtaaggat tccttcagta agatcaacat cctcgcgatt aagccggaca
      721 atttcagtcc tacgcaatcc gcagccgtaa gcgatagaaa gtaaggctcg ctccctttta
      781 tctgcacagg cgttaaataa aaggcgaatt tcagcaaggg tgaccacgtt acgtgaaacc
      841 gattttgcca taatgaattt cggaatgtga acaggacttc cttccaattc tccggaatct
      901 atcaggtgat caaataaaag ccgtaaggca aacaattgcg atctgataac tgcatcactc
      961 aagccaccct ccctccgttg gttcggtctt tcatgtagat actcaaaata atttaccacg
     1021 tgtttcgctt ttaccttcgt aactgaatcg atcccatttg attctatgaa atagaagaac
     1081 tcgcgtacaa aattcggata gctgctatcc ttaccccgtg agaacccttt cgctaaaacc
     1141 atctgtgtaa aatcaacgta gatttttttc aattcctcac tatgtaaaac caaatgcttc
     1201 atttcgtata attgcggtca gcgaccgttt tttcattttc tattttcact acgccctttt
     1261 tttttctata caatgcgcac tgctaaactt ttggaacacc accgatcaat ccttagacac
     1321 gtgcgggttt tggtgttcca aaccctctga accaccaagt gccaccagtt gtttttgcag
     1381 ttcgcacttg attttttccc ttgttttctc catgtcatcc caatagacaa ttttgtattt
     1441 gaatccccgg ttggcataac cacctgttcg ctgaatgtat tccaaaagtt ccagttgctc
     1501 gaaataccgg aagcattgcg ttttgcttac gttgagcgca agccttacat cgcgctgcgt
     1561 aaagtcggag gccgactgcc atttttcttt ta
//
LOCUS       DAOMYG010000561         1589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_31122_length_1589_cov_8.890482, whole genome shotgun sequence.
ACCESSION   DAOMYG010000561 DAOMYG010000000
VERSION     DAOMYG010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31122_length_1589_cov_8.890482"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PK610_13720"
     CDS             <1..291
                     /locus_tag="PK610_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRJ37049.1"
                     /translation="FDEIDTGVSGEVAHKMGEIMKKMGQSMQVFSITHLPQVAVKGNA
                     HYKVYKETGKQSTQTKMQRLSDQQRLEEVARMLSGDKLSDAALANARELMMA"
     gene            294..695
                     /locus_tag="PK610_13725"
     CDS             294..695
                     /locus_tag="PK610_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37050.1"
                     /translation="MDDPGFFYIAVSEIVFLSLIGFIAGWLIVMDAVPQPKNAVKWSV
                     ALFFAALLAVMASNTPVMIVSLFQIKPYFLLVFGIVFGFYLNRYNDRLAEEKRTVAKV
                     LLTYIGSLSGLIFLNMLTYSAALLLIYLRSS"
     gene            complement(908..>1589)
                     /locus_tag="PK610_13730"
     CDS             complement(908..>1589)
                     /locus_tag="PK610_13730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRJ37051.1"
                     /translation="DGNPTVRANLLVDATRFIGYPNIAWAGYQEGDAQAIIGFNYVSS
                     TEPGGTGTVLFRDDSTYSNFVTLKQGNSYVNVISGTGERWGDYSGIQRRYNQPCTAWM
                     AGTFGKAVNANGTWIAEVSSEISCSANPVAGISDESKSISGMNVFPNPGNERIQFDFT
                     LEQTTIGSIRVLDMQGRIVCVLLEDRIKAGKNRLSFDTSQFPAGYYLISIAMSNGENK
                     QIKFAVAR"
BASE COUNT          412 a          345 c          356 g          476 t
ORIGIN      
        1 tttgatgaaa ttgataccgg cgtttccggg gaggtggcac acaaaatggg cgagatcatg
       61 aaaaaaatgg ggcaaagcat gcaggtgttt tccattacgc acctcccaca ggtagctgta
      121 aaaggaaatg cgcattataa agtctacaag gaaaccggaa agcaaagtac gcaaacaaaa
      181 atgcagcgac tcagcgatca gcagcgcctc gaagaggttg cacgtatgct gagtggtgat
      241 aaattgtcgg atgcagcatt agccaatgcg cgtgagttaa tgatggcctg aatatggacg
      301 accccggatt tttttacata gcggtttcag agatcgtatt tctttccctg attggtttca
      361 tcgcaggatg gctgattgta atggatgccg taccacaacc gaaaaatgca gtgaaatggt
      421 cggtggctct tttttttgca gctttgctcg ctgtaatggc aagtaatact ccggttatga
      481 tcgtctcact ttttcaaatc aaaccttatt ttttattggt attcggtatc gtcttcggtt
      541 tttatctgaa ccgttacaac gaccggttag ctgaggaaaa aagaactgtt gcgaaagtat
      601 tgctaactta catagggagt ttgtcaggat tgattttcct caatatgctc acatattctg
      661 ctgctctatt gctcatttat ctgcggagta gctgaggatt tcctgttttt tttcgggtat
      721 tcgcgtaaag gattgaagta gtagccaatg ctaaataccg cttccaaacc agcatagggc
      781 ggtggttttc ttagattttc tctgttattt cccgggtgat tttcgaattt gtcctggata
      841 cgggtgattt ctttattgat accgattcca aaaaatgtgg agagataaaa acgtttcgaa
      901 attctcctca ccgcgcaacc gcaaatttga tttgcttgtt ttcaccgttc gacattgcaa
      961 tgctgatcaa atagtatcct gcaggaaatt gagaagtatc aaaactaagt ctgttctttc
     1021 ctgctttgat tctgtcctca agcaatacac aaactattct tccctgcata tccaaaacgc
     1081 ggatgcttcc aattgttgtt tgttccaggg tgaaatcgaa ctgaatgcgt tcattacccg
     1141 gattggggaa tacattcatt ccgcttatgc ttttgctctc atcagaaatg cctgcaacag
     1201 gattcgctga acaacttatt tcagaggaaa cttctgcaat ccacgttccg ttggcgttta
     1261 ccgctttccc gaatgtgccg gccatccatg cggtacacgg ttggttgtag cgacgctgta
     1321 ttcccgaata atctccccaa cgctctccgg tacccgaaat gacattgacg taactgttgc
     1381 cttgtttcaa ggtgacaaaa ttcgaatagg tggaatcgtc tctgaacaat acggttcctg
     1441 ttccaccggg ttcggttgag gatacgtaat tgaaaccgat gatggcctgc gcatctcctt
     1501 cctgatatcc tgcccaggca atattcggat aaccgataaa ccgggttgca tctacaagca
     1561 gattcgcacg cacagtagga tttccatct
//
LOCUS       DAOMYG010000562         1579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_31454_length_1579_cov_6.744751, whole genome shotgun sequence.
ACCESSION   DAOMYG010000562 DAOMYG010000000
VERSION     DAOMYG010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31454_length_1579_cov_6.744751"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PK610_13735"
     CDS             complement(<1..210)
                     /locus_tag="PK610_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37052.1"
                     /translation="MLHKGSRLIESYLLFLKSSRLSDQTIRSYRCIVEKFLAENPGAV
                     NFTYSDILFARLVDLGTATSLRPNEF"
     gene            complement(200..1144)
                     /locus_tag="PK610_13740"
     CDS             complement(200..1144)
                     /locus_tag="PK610_13740"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRJ37053.1"
                     /translation="MKHLPLHSDEFLKAHADFRQMILAKGFARGKDNNYPNFIREFFF
                     FLESIGVDTLQKVKSKHVAAYYAYLHERPNQRRGGGLSDSIIRAQLFALRLLFDHLMD
                     AGLLDGSPVHLPKFMMGSSGQRNVVSLAEIQLLFKACCDKRERAILSVAYGCGLRRTE
                     MVRLNREDVDLTEGLLTVRSGKFGKTRTVPLSDFVVKDLREYVIEERPQLLECGSGTQ
                     SFFVNNWGNRMQGLAMNNYLKAIIERTGDLKLKQKGITLHCLRHSIATHLLDNGAEME
                     FVQRMLGHTMIDTTHIYSKRRKQKNIILEQFKNKHHAA"
     gene            complement(1293..>1579)
                     /locus_tag="PK610_13745"
     CDS             complement(1293..>1579)
                     /locus_tag="PK610_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37054.1"
                     /translation="KEKWQSASDFTQRDVRLALNVSKTQCFRYFEQLELLEYIQRTGG
                     YANRGFKYRVVYWDDMEKTREKIKGELQKQLVALGGLNDKEHQNPQVSKD"
BASE COUNT          420 a          375 c          299 g          485 t
ORIGIN      
        1 aaactcatta ggacgcagcg acgtggctgt tccgaggtca actaggcgag cgaataagat
       61 atcgctgtac gtgaaattaa ctgctcccgg attttcagct aagaattttt ccacgatgca
      121 gcggtaagag cgaatagtct gatcagaaag acgactgctt tttaaaaata gcaagtaaga
      181 ttcaattaaa cgagatcctt tatgcagcat ggtgcttgtt tttaaattgt tcgagaatga
      241 tattcttttg ttttctgcgc ttactgtaaa tatgcgtggt atcgatcatc gtatgaccaa
      301 gcatacgttg cacaaattcc atttctgctc cgttatccag taagtgcgtg gcaatactat
      361 ggcgaaggca atgaagcgtg attccttttt gttttagttt cagatcaccc gtgcgctcaa
      421 tgattgcttt cagataattg ttcatggcca atccctgcat gcggtttccc caattgttta
      481 caaagaagga ttgtgtacct gatccgcatt ccaaaagctg tggtctttcc tctatgacat
      541 attcccgcag atctttcact acgaaatctg aaagcggaac ggttcgcgtt tttccgaact
      601 taccggagcg aaccgttaaa agtccttcag taagatcaac atcttcccgg tttaagcgca
      661 ccatttcggt tctacgcaat ccgcagccat aagccactga taatatagcc cgctctcttt
      721 tatcgcaaca ggccttaaat aaaagctgaa tttcagcaag cgatacaacg ttgcgttgtc
      781 ccgaactacc catcatgaat tttggaaggt gaaccggact cccatccaat aacccggcat
      841 ccatcaggtg atcaaataaa agtcgtaaag caaaaagctg cgccctgatt atcgaatcgc
      901 tcaagccacc acccctgcgt tgattaggtc tttcatgtaa atacgcataa taagccgcta
      961 catgtttcga ttttactttt tgcaaagtat caactccaat cgactcaaga aaaaagaaaa
     1021 actcacgaat aaaattcggg taattattat ccttaccacg cgcaaatcct ttggccaaaa
     1081 tcatctgcct gaaatccgca tgtgctttca aaaattcatc gctatgtaat ggcaggtgtt
     1141 tcatttaatt tttatctttt taactccgga ttcccggatt tttttagttt tgtttttcac
     1201 ttttttcata agcgacttat gaagtcattt tttacgaagc cgttattttc ccctatacaa
     1261 tgcgcactac tgaacttttg gaacaccacc gatcaatcct tagacacctg cgggttttgg
     1321 tgttccttgt catttaaacc accaagtgcc accagttgtt tttgcagttc gcccttaatt
     1381 ttttcccttg ttttctccat gtcatcccaa tagaccacgc ggtacttgaa tccccggttg
     1441 gcataaccgc ccgtgcgctg gatgtattcc aacaattcca gttgctcgaa ataccggaag
     1501 cattgcgttt tgcttacgtt cagggcaagc cttacatcgc gctgcgtaaa gtcggaggcc
     1561 gactgccatt tttctttta
//
LOCUS       DAOMYG010000563         1570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_31726_length_1570_cov_5.844224, whole genome shotgun sequence.
ACCESSION   DAOMYG010000563 DAOMYG010000000
VERSION     DAOMYG010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31726_length_1570_cov_5.844224"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="PK610_13750"
     CDS             <1..952
                     /locus_tag="PK610_13750"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HRJ37055.1"
                     /translation="VPFVSNYYSPEFEKLAYYFEIYNSLRLGEGELFLLTQFVREKRT
                     GETVPGLSKSQRQSVKEVIPVLTVFDISKLASGTYELVVEARNKNNEIIAVNSWEFQR
                     NNPETDISLESLLKTDIKATFVDKITNSDTLNEYIFCLRPIALPAEILFIDSVARIQD
                     MNFKKQFFYQFWKNREPVTPEYAWLRYKSTVAMVEKLFATSIKRGYQTDRGAIYLKYG
                     SPDWVLDRPNEPSSYPYQVWQYKKLGQFNNKRFIFYLPDLVTNDYVVLHSDVPGEVRN
                     HRWQQMLNARNSPNIDIDNPWGGNNPHYGGNAGEFFQNPR"
     gene            952..>1570
                     /locus_tag="PK610_13755"
     CDS             952..>1570
                     /locus_tag="PK610_13755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRJ37056.1"
                     /translation="MRPSVIVALLNWNGKKHLAEFLPSVVSHSGDARIVVIDNASTDD
                     SVAFLKSNFPNVEIILNKSNSGYAGGYSEGLEKLDADYFILLNSDVEVTPNWIDPVVQ
                     HMESDKKVAAAQPLIRSYSSRTHFEYAGAAGGFLDKDHYPFCRGRMFETAEEDRNQYS
                     SVREIFWATGACLFVRASAYREVGGLDPDFFAHMEEIDFCYRLKNR"
BASE COUNT          457 a          310 c          350 g          453 t
ORIGIN      
        1 cgttccattt gtttcgaatt attattcgcc ggagtttgaa aagctcgctt actattttga
       61 aatctacaat tcacttcgtc ttggtgaagg cgaattgttt ttgctaaccc aatttgtgcg
      121 cgaaaaacgc accggcgaaa ctgttcctgg attgtccaaa tcacaacgac aatccgtaaa
      181 agaggtgatt ccggtattaa cggtttttga tatctcaaaa cttgcatcgg gcacttatga
      241 attggttgta gaggccagaa ataaaaacaa tgaaattatt gctgtcaact cctgggaatt
      301 tcagcgcaat aatcctgaaa cagatattag tttggaatcg ctgctaaaaa cggatattaa
      361 agcaactttc gtagataaga taaccaattc agatacgctt aacgaataca ttttttgttt
      421 gcgtccaatt gcattgccag cggaaatatt atttatcgat tctgttgcca gaattcagga
      481 tatgaatttt aaaaagcagt tcttctatca gttctggaaa aacagagaac ccgttactcc
      541 ggaatatgcc tggctgaggt ataaatcgac cgtggcaatg gtggaaaaac tttttgcgac
      601 cagtattaaa cgtggttacc aaaccgatcg cggtgcaatt tatttaaaat acggatctcc
      661 ggattgggta cttgaccgtc cgaatgagcc gagctcttat ccttaccagg tttggcaata
      721 caaaaagctg ggacagttca ataacaaacg atttattttc tatttgcccg atctggtaac
      781 caacgattat gtggttttac attcagatgt gcccggagaa gtacgtaacc atcgctggca
      841 gcaaatgctg aatgcacgca acagtccgaa tatcgacatc gataatccat ggggaggaaa
      901 taaccctcac tatggcggta atgccggaga attttttcag aatccccgtt gatgcgtcct
      961 tcggttattg tagcgctgct caactggaac ggaaagaaac atcttgccga atttcttcca
     1021 tcggttgtaa gtcattccgg agatgcaaga attgttgtga tcgataatgc ttccaccgac
     1081 gattctgtag catttctgaa atcgaatttt cctaacgtag aaatcatcct caacaaatcg
     1141 aattccggtt atgcgggtgg atacagtgaa ggactcgaaa agctggatgc agattatttt
     1201 atacttctca attcggatgt tgaggttaca cccaattgga tcgatccggt ggtgcaacat
     1261 atggaaagcg ataaaaaagt tgcagctgcc caacctttaa ttcgttctta ttcctctcgt
     1321 actcatttcg aatatgcagg agcagcagga ggatttttgg ataaggatca ttatcccttt
     1381 tgcagaggga gaatgtttga aacagcagaa gaggacagga accaatacag cagtgtaaga
     1441 gaaatctttt gggcaacagg agcatgtttg tttgtgcgtg cctctgccta tcgggaggtg
     1501 ggtggactcg accccgactt ttttgcgcat atggaggaga tcgatttttg ctaccgttta
     1561 aaaaacaggg
//
LOCUS       DAOMYG010000564         1558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_32091_length_1558_cov_12.403859, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000564 DAOMYG010000000
VERSION     DAOMYG010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32091_length_1558_cov_12.403859"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..1362
                     /locus_tag="PK610_13760"
     CDS             184..1362
                     /locus_tag="PK610_13760"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRJ37057.1"
                     /translation="MTEAYIVAAYRTAVGKANRGGFRFMRPDDLAAQVIRHMMAQYPQ
                     VEKERIDDVIVGNAMPESEQGLNMGRFISLMGLDTEKVPGMTVNRYCASGLETIAIAT
                     AKIRSGMADCIIAGGSESMSLIPMGGWRVVPHPEVSAEHPDWYWGMGLTAEAVANEYK
                     ISREDQDAFALHSHQKAIAAITAGKFKEEIVPVKVKESYLDGNMKKKVKEYVVDTDEG
                     PRADTNPEALAKLKPVFSATGSVTAGNSSQTSDGAAFVMVMSERMVKELGLKPIARLV
                     NYAAAGVPPRIMGIGPVAAIPKALKLAGLKQNDIGLFELNEAFASQSLAVVRELGIDP
                     SRVNVNGGAISMGHPLGCSGAKLSVQLFSELKRRNEKYGMVTMCVGTGQGAAGIFEML
                     "
BASE COUNT          523 a          322 c          346 g          367 t
ORIGIN      
        1 accgacaact tttcatcagt acttaaggaa atgggatatc tacctgtttt acccatggaa
       61 ttatcctaac tattagagga gagcaaacgt gaacaactga aaaaggaaaa aaatataatc
      121 agcagatttc gaaagggaga tctgaacatt gaaaatcatt aacgaacaaa acaccattta
      181 atcatgacag aagcatatat cgtagccgcc taccgaactg cagttggaaa agccaatcgc
      241 ggaggattcc gcttcatgcg tcccgatgat cttgcagcac aagtgatccg acacatgatg
      301 gcgcagtatc ctcaggtaga aaaagagcgc attgatgatg taatagtagg aaacgccatg
      361 cccgaatcgg aacagggatt gaacatggga cgattcattt ctcttatggg tcttgacacc
      421 gaaaaagtac ccggaatgac agttaatcgc tattgtgcat ccggattgga aacgatagca
      481 attgctactg cgaaaatccg ttcaggaatg gcagactgca ttattgccgg tggttcggaa
      541 agcatgagtc ttattccaat gggtggatgg cgtgtagttc cccaccccga agtatctgca
      601 gaacatcctg attggtattg gggaatgggt ttaactgcag aagctgttgc caatgaatac
      661 aaaatttcga gagaagatca ggatgcattt gccttgcatt cacatcaaaa agcaattgca
      721 gctataacag caggaaaatt taaagaagaa atcgttccag taaaagttaa agagagttac
      781 ctcgatggta acatgaagaa aaaggtgaag gaatatgtag tagataccga tgaaggtccg
      841 cgtgccgaca ccaatcctga agcgcttgca aaacttaaac ctgtgttttc ggcaacagga
      901 tcagttacag caggaaattc atcacaaaca agcgacggag ctgcatttgt aatggtgatg
      961 agtgagcgca tggtaaaaga gcttggatta aaacccattg cacgactcgt gaattacgcc
     1021 gctgcaggag taccgcctcg tattatggga ataggacctg tagcagcaat tccaaaagcg
     1081 ctaaaacttg caggcttaaa acaaaacgat atcggactct tcgaactcaa cgaagcattt
     1141 gcatcgcaat cgctggcagt tgttcgtgag ttgggtattg atccttcaag agtaaatgtg
     1201 aacggaggag cgatttcaat gggacaccca ctcggttgtt ccggagcgaa actcagtgtt
     1261 caattgttca gcgaattaaa acgcagaaat gaaaaatacg gaatggtcac catgtgcgta
     1321 ggaaccggcc aaggcgccgc cggaattttt gaaatgctat aaccaattat ccaatgacca
     1381 atttccaatt aaatccttta gcgaaatcga ctgcaaggag atttctgctt caggaaacca
     1441 ttcaccgata accaataact gataaccaat tactgataac tgataactga taactgataa
     1501 ctgataactg ataactcata accaataact cataactcac aaccaataac ttccaccc
//
LOCUS       DAOMYG010000565         1557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_32127_length_1557_cov_5.812916, whole genome shotgun sequence.
ACCESSION   DAOMYG010000565 DAOMYG010000000
VERSION     DAOMYG010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32127_length_1557_cov_5.812916"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PK610_13765"
     CDS             complement(<1..418)
                     /locus_tag="PK610_13765"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRJ37058.1"
                     /translation="MRIIGGKFKGRKLQAPLLPDTRPTTDFARESLFNLLQTRFDLDG
                     IKVLDLFAGTGAVSFEFISRGAAMATCVDTSPKALQFIRDAAKQLGIEIRTNRSEVER
                     FLKSDSSSYDIIFADPPYTMADLSWIPKMVFEKNLLR"
     gene            complement(409..1185)
                     /locus_tag="PK610_13770"
     CDS             complement(409..1185)
                     /locus_tag="PK610_13770"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HRJ37059.1"
                     /translation="MSEKNLQNNHLSIEFGGGFSFLASYNRSERSLNSARILAPAPGD
                     KDCLNALRTDELFISGPSQASLSFCNGKFVMMPNALFAPEHARAMLQLNASINDDDEV
                     RHMDMPAFDAQLIYAASREVEKEMTKRYPRYHEGHCAGHFLSLCRRQLKDGQQVFLYF
                     YPGFFLMSAFIGEQLQMINAYSWRDPDDVLYFSLYACEQIRFDPAQVRFSAGGEIEKE
                     GKVHRLLERYTTGIQFMESPGIISNESARFLTLSEQFRCA"
     gene            complement(1195..>1557)
                     /locus_tag="PK610_13775"
     CDS             complement(1195..>1557)
                     /locus_tag="PK610_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37060.1"
                     /translation="LGYKEHPEKMISEVEAWKLAKAGLLSNFNRDTTYNSVMEKIFLS
                     NKALASRDSDYKFSVDSLDVAPYSGGMKFSMETGMIKKNNIDVPVLLIVEPNPFDKEK
                     PLTVGSLTDVTTNGNWGE"
BASE COUNT          429 a          347 c          354 g          427 t
ORIGIN      
        1 cgcggagtaa atttttttcg aaaaccattt ttggaatcca gctcagatct gccattgtgt
       61 aaggaggatc cgcaaaaatg atgtcgtaag aagaggagtc agatttcaga aaccgttcca
      121 cctcagaacg attggtacgt atttcaatgc ccaattgttt cgcagcatcg cgaatgaact
      181 gtagtgcttt cggtgaagta tccacacagg ttgccattgc cgcacctctg gagatgaatt
      241 cgaaacttac cgctccggtt cctgcaaaaa gatcgaggac ttttattcca tccagatcaa
      301 atcgtgtttg aagcaggttg aacaggcttt ccctggcaaa atcggtagta ggacgcgtat
      361 caggcagtag tggagcctgg agttttctcc ctttgaattt tcctccgatt atgcgcacct
      421 gaactgttcg cttaaagtga gaaaacgggc ggattcattg ctgattatcc ccggcgattc
      481 cataaactga attcctgttg tgtagcgttc aagcaagcgg tgcacttttc cctctttttc
      541 gatctcgcca cctgccgaaa atcgaacttg agccggatcg aaacggatct gctcgcaggc
      601 gtacaaactg aaataaagca catcatccgg atcgcgccat gagtaggcgt tgatcatttg
      661 caattgctca ccgataaaag cactcatcag aaaaaatccg ggataaaaat aaagaaagac
      721 ttgttgtcca tcctttaact gacggcggca aagcgaaaga aaatgtccgg cgcaatgtcc
      781 ttcatggtac cgcggatatc gctttgtcat ttccttttct acctcccttg aagcagcgta
      841 aatcagctgt gcgtcaaaag caggcatatc catatgtcgg acttcatcat catcatttat
      901 ggatgcattc aattgaagca ttgcacgcgc atgttcagga gcgaacaagg cattaggcat
      961 catcacaaat tttccattgc aaaagctgag ggaggcctgt gatggtccgc tgataaagag
     1021 ttcatctgta cgaagtgcat ttaaacaatc cttatcgccc ggagcaggag caaggatgcg
     1081 tgcagaattg agcgaacgct ctgaacggtt atagctcgcc agaaaagaaa aaccaccgcc
     1141 gaattctatc gagaggtggt tgttctgaag atttttttcg ctcatcaggg gaatttattc
     1201 tccccagtta ccattggtgg tgacatccgt caatgaacca acagtaaggg gtttttcctt
     1261 atcaaaagga tttggttcta caattaaaag aaccggaaca tcaatattgt ttttcttgat
     1321 cattcctgtt tccattgaaa acttcattcc gccggaatag ggagcaacat caagtgaatc
     1381 cacgctgaat ttgtagtcgg aatcgcggct ggcgagggcc ttgttggaaa ggaagatctt
     1441 ttccattacc gaattgtaag tggtatcgcg attaaagttt gatagcaaac cggcttttgc
     1501 aagtttccat gcttcaactt cagagatcat tttttcagga tgctctttgt agccaag
//
LOCUS       DAOMYG010000566         1524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_33153_length_1524_cov_8.234854, whole genome shotgun sequence.
ACCESSION   DAOMYG010000566 DAOMYG010000000
VERSION     DAOMYG010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33153_length_1524_cov_8.234854"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="PK610_13780"
     CDS             <1..1325
                     /locus_tag="PK610_13780"
                     /inference="COORDINATES: protein motif:HMM:NF017142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37061.1"
                     /translation="TDNLQQITLKARNYHQLIDCPMMFSPADTAQFFVNKTKVTISVY
                     DVQGQRRAKQFYNALKRDMEAIAAFLPELPVDSYTFIIFVDDLREIGQAFTGKIGLGK
                     RIKLAKKFGNLGLGALEHGNSSTYYLGDFGPGLDIEDLSLEGQLTGAAIHEFMHIVTP
                     LGLHSQHIGNFNYTNPVMSKHLWLYEGITEYFAHLVKYKGGVYTPDQFLKEMSLKFSS
                     GVSFPIQEMSFTEMSANVLQPKYEKHYGQVYQRGAAMGMLLDAEIQRLTDGKKELIDV
                     ILTLVNRYGAEKSFDEESFIDEFVAEVHPDLKNYFTLYVEGKNQWKPNDQLIGMKVVY
                     YDTLRENSLLNPLSPYDNEIRTSDSKGGLEKTITQVGSGEFVGFRPGDKVDVLEFLKA
                     LKKPNGDPVDPDQEVIVTVIRDNQPKKLMFKARYGMKTKVHVLKWIQR"
BASE COUNT          483 a          286 c          334 g          421 t
ORIGIN      
        1 ccacagataa tttacaacaa atcactttaa aggcacgcaa ttatcatcag ctgatcgatt
       61 gtccgatgat gttcagtccg gccgacacgg cgcagttttt tgtaaacaaa acgaaagtca
      121 ccatttcggt ttacgatgtt caaggccagc gcagagcaaa acaattttac aatgcactta
      181 agcgtgacat ggaagctatt gcagcatttc ttccggagtt gcctgttgac agctacacct
      241 tcatcatttt cgtagatgat ctgcgtgaaa ttggacaagc ctttaccggt aagattggat
      301 tgggaaagcg aattaaactc gcaaaaaaat tcggcaacct cggactgggt gcactggagc
      361 atgggaattc ttccacctat tatctgggcg attttggtcc tggtctggac attgaagatc
      421 tttcgctgga aggacaatta accggtgcag ccattcacga attcatgcat attgttactc
      481 cgttaggatt gcattcacag catattggaa actttaacta taccaatcct gtaatgagta
      541 agcacctttg gttgtatgaa ggaataactg aatactttgc ccatctggta aaatacaaag
      601 gtggagttta tacaccggat caattcttaa aggaaatgag tctgaaattt tcatccggtg
      661 taagtttccc cattcaggaa atgtcgttca ccgagatgag tgcaaatgta cttcaaccga
      721 aatacgaaaa acattacgga caagtctacc agcgtggtgc agcaatggga atgttactgg
      781 atgccgaaat acagcgactg acagatggca aaaaagaatt gatagatgta attcttacgc
      841 tggtaaaccg gtacggagct gaaaaatcct ttgatgagga atcgttcatt gacgaatttg
      901 ttgcagaagt tcatcctgat ttgaaaaatt atttcacact ttacgttgaa ggtaaaaacc
      961 aatggaaacc caatgatcag ttgattggga tgaaggtagt ttattacgat acccttcgcg
     1021 aaaattcatt gttgaatcct ttatcacctt acgacaatga aatccgcacc agcgattcga
     1081 aaggaggatt ggaaaaaaca attactcagg ttggatccgg agaatttgtt ggttttcgtc
     1141 cgggcgacaa agtggatgtt cttgaatttt tgaaggccct taaaaaacca aatggagatc
     1201 ctgtagatcc ggatcaggaa gtgatcgtaa ctgtaattcg cgacaaccaa cccaagaagc
     1261 taatgtttaa ggccagatat ggcatgaaaa caaaagttca tgtattgaaa tggatacagc
     1321 gttgatggtg ttttttgtaa atggaattta agtatcgatt ttgaattttt gaaataaatt
     1381 cagaactact tattcctttt tcccggcgaa taaacaaacc ggagaaggag aagagcaact
     1441 accagaattc caccaaaaat gtattcaagg gtaaagttgg aagtgcgttc ctgaatctgt
     1501 ccaagttcag gatccggttc agga
//
LOCUS       DAOMYG010000567         1523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_33192_length_1523_cov_10.972752, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000567 DAOMYG010000000
VERSION     DAOMYG010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33192_length_1523_cov_10.972752"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1523
                     /gene="mutS"
                     /locus_tag="PK610_13785"
     CDS             <1..>1523
                     /gene="mutS"
                     /locus_tag="PK610_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010134431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRJ37062.1"
                     /translation="LNHLFKALQVLLPLKEFCSKSEVKALKTIGEQINPCDSIRDRIE
                     QELDPEAPMAIGKGQVIRKGINSELDELRELMYSGKDYLLKIQQREAEKTGIPSLKVA
                     FNNVFGYYLEVTNSHKDKVPSEWIRKQTLTNAERYITEELKEYETKILGAEEKIFALE
                     SRLFNELISALATFILPIQHNAYLLAQLDVFQSYARTAIQNNYTRPEINEGYVLSIKN
                     GRHPVIEKALPEGTSYIPNDLYLDNDQQQIIMITGPNMSGKSAILRQTALIVLMAQMG
                     SFVPAQHAQIGIIDKVFTRVGASDNLSSGESTFMVEMNETASILNNISQRSLILLDEI
                     GRGTSTYDGISIAWAIAEYLHENGKSKPKTLFATHYHELNEMERQYPRIKNFNVSVKE
                     TGNKILFLRKLIAGGSEHSFGIHVAKLAGMPSKVVNRANEILHRLEETRADAGNSGGT
                     TIQALPEEKDAMQLSFFQLDDPVLSQIKDEIQQTDINTLTPVEALMKLNEIKKLIGG"
BASE COUNT          532 a          296 c          308 g          387 t
ORIGIN      
        1 actcaatcat ctgtttaagg ctttacaagt tcttttacca ttgaaagaat tttgcagtaa
       61 aagcgaggta aaagcactaa aaaccattgg tgaacaaatc aatccctgcg acagtatccg
      121 cgatcgaatt gaacaagaat tggacccaga agctccaatg gctattggaa aagggcaagt
      181 gatccgaaag ggaatcaatt ctgaactgga tgaactcagg gaattgatgt attctggaaa
      241 agattaccta ctgaaaatac aacaaagaga agctgagaaa acaggaatcc cgtcactcaa
      301 ggttgcattc aataacgtat ttggatatta tctcgaggtg actaattccc ataaagacaa
      361 agtgccatcg gaatggattc ggaaacagac gctaactaat gctgaaagat acatcacaga
      421 agagctgaaa gagtacgaaa ctaaaatttt aggggcagaa gaaaagattt ttgcacttga
      481 atcacgcttg ttcaacgaac ttatttctgc actggcaact tttattttac ccattcaaca
      541 caatgcatat ttattggcac aactcgacgt tttccagagc tatgcacgaa cagcaataca
      601 aaacaactat actcgtcccg agatcaatga aggatatgtt ctatccatta aaaacggaag
      661 acatcctgtt attgaaaaag cattgcctga aggaacatcc tacattccaa atgaccttta
      721 tctggataat gatcagcagc aaattattat gatcaccgga ccgaatatgt caggtaagtc
      781 tgcaattctt cggcaaacag ctcttattgt tctgatggcg caaatgggca gttttgttcc
      841 tgcacagcat gcgcaaattg gaatcataga caaagtattt acacgggttg gagcttctga
      901 taatttatcg tcaggagaat ccacattcat ggtagaaatg aatgaaaccg ccagcattct
      961 gaataatata tcgcaaagga gtctgattct tttagatgaa attggtcgcg gaacaagcac
     1021 ctacgacggc atcagtattg cgtgggcaat tgctgaatat ttacatgaaa acggaaagtc
     1081 aaaacccaaa actttatttg ccacccatta tcatgaactg aatgaaatgg agcggcaata
     1141 tccgcgtatt aaaaacttca atgtttctgt caaggaaaca ggaaacaaaa tattgttttt
     1201 acgaaaactg attgccggag gcagcgaaca cagtttcggt attcacgttg caaaactggc
     1261 aggaatgccc tctaaagttg tgaaccgtgc aaacgaaatc ctgcaccgac tcgaagaaac
     1321 acgtgctgat gccggaaact ccggaggtac aactattcaa gcattgccgg aagaaaagga
     1381 tgctatgcaa ttaagtttct ttcagcttga cgacccggtt ctttcgcaaa taaaagacga
     1441 aattcagcaa acggatatta atactttaac accggttgaa gcattgatga aactaaatga
     1501 aattaaaaaa ctaattgggg gtt
//
LOCUS       DAOMYG010000568         1522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_33233_length_1522_cov_10.268575, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000568 DAOMYG010000000
VERSION     DAOMYG010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33233_length_1522_cov_10.268575"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PK610_13790"
     CDS             complement(<1..709)
                     /locus_tag="PK610_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37063.1"
                     /translation="MNAIRATGLLVVLTGVLLMASCRNETKSVPKKRFAAAGDTSDLF
                     LHKSIPTHAQGFIVEYHPTFKKVKVVDPSDSTIVFGTYILAERGKSFSATTTDIVVEI
                     PLTSVASVSTTHLAFLGHLNVLDALTGFSGMKYVQHKDVLSRYKKGLIKEIGRETDLN
                     KEKIFELAPEILMVYPYEGMDYSWAQKAGIPVVYNSDYLELTPLGKAEWIKFFALFFN
                     EEEKANEWFKKVENEYIA"
     gene            complement(706..>1522)
                     /locus_tag="PK610_13795"
     CDS             complement(706..>1522)
                     /locus_tag="PK610_13795"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRJ37064.1"
                     /translation="VNWDNIAPAGAINSTVNDMLKWIRLQLNRGQLDNKVFWSERQSR
                     EMWLPQTIDNVSSFSERLHPSKHFSAYGLGWDLFDFYGYKIVNHSGGLDGMISHVFMI
                     PELNIGCVILSNSATSLPYALMHQYLEILIGDPNENDWSATYLGFAKGYEEYVEEKNK
                     EKERNRKKGVSPTFDMKNYTGVYSGNVYGNAKVDIREGKLYLRLMHTPTMQGYLNHWE
                     ADVFSIVLTDHPSLPKGEVTFVLDEDKTKVMRLVIDIPNPDFDFTELDFNKIK"
BASE COUNT          443 a          356 c          266 g          457 t
ORIGIN      
        1 gagcgatgta ttcgttctca actttcttaa accattcatt cgctttttct tcctcattaa
       61 aaaacaaagc gaaaaacttg atccattccg cctttccaag cggagtcaac tccagataat
      121 ctgaattata cacgaccgga ataccggctt tttgggccca ggaataatcc attccctcgt
      181 agggatacac catcaatatt tcgggtgcca actcaaaaat tttctcctta ttcagatccg
      241 tctcccgtcc aatctccttg attaaccctt ttttataccg gctcaataca tctttatgct
      301 gcacatattt cataccgctg aatccggtta acgcatccaa aacattcaga tgccccagaa
      361 atgccagatg agtggttgac acactcgcta cactggtaag tggaatttcc acaacgatat
      421 ctgtggtagt tgcagaaaat gatttgcctc tttccgcaag aatatacgta ccgaaaacaa
      481 tagttgaatc ggaggggtct acaactttta ctttcttaaa agtcgggtga tattcaacaa
      541 taaatccctg cgcatgcgtt ggaatggatt tatgcaaaaa caaatccgaa gtatctcctg
      601 ctgctgcaaa tcgcttttta gggacagact tcgtttcgtt ccgacaagag gccatcaaaa
      661 gaacgcccgt caatacaacc aatagacctg tcgctcttat tgcgttcatt ttattttatt
      721 aaaatccagt tccgtaaaat caaaatcagg gtttgggata tcaatcacta aacgcattac
      781 tttggtctta tcctcatcca atacaaaagt gacttctcct tttggcaatg aagggtgatc
      841 ggttaaaaca atactaaaaa catctgcctc ccaatgattc aaatatcctt gcatggttgg
      901 agtgtgcatc aatcgtaaat acaattttcc ttcacgaata tccactttag cattgccata
      961 tacatttcca ctatatacgc cggtgtaatt tttcatgtcg aaagtcggac ttactccctt
     1021 tttgcggtta cgttcctttt ctttgttttt ttcttcgaca tactcttcat atccctttgc
     1081 aaagccgagg taggttgcgc tccaatcgtt ctcattggga tcgccaatca aaatttccag
     1141 atactgatgc atcaatgcat agggtaaact ggtggcggaa ttggagagaa ttacacaacc
     1201 gatattcaat tcaggaatca tgaatacatg tgaaatcatg ccatctagtc ctccgctatg
     1261 gttgacaatc ttgtaaccat aaaaatcaaa cagatcccaa cccaatccgt aagcagaaaa
     1321 atgtttggaa ggatggagtc gttctgaaaa agaagaaaca ttatcgatgg tttgcggtaa
     1381 ccacatttcg cgggattgac gttcgctcca aaacacttta ttatccagtt gccccctatt
     1441 gagctgaagt ctgatccatt tcagcatatc gttcacggta gagtttatgg cacccgccgg
     1501 agcaatatta tcccagttta ca
//
LOCUS       DAOMYG010000569         1522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_33235_length_1522_cov_8.978187, whole genome shotgun sequence.
ACCESSION   DAOMYG010000569 DAOMYG010000000
VERSION     DAOMYG010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33235_length_1522_cov_8.978187"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PK610_13800"
     CDS             <1..347
                     /locus_tag="PK610_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37065.1"
                     /translation="VRGYEYYVIDGQNFAMFRSNVKYRLFGPKIHDLDFLQGTNFSKL
                     HYAFYLNLYIDAGYVQDRRYYTENPLANQMLYGTGIGLDFVSFYDKVIRFEYSMNKEL
                     EKGFFIHFIKPI"
     gene            349..1227
                     /locus_tag="PK610_13805"
     CDS             349..1227
                     /locus_tag="PK610_13805"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HRJ37066.1"
                     /translation="MTIAIYGRSFSEEHIPHIQQLFDELAKRKAQLIVFEAFNSFLRP
                     RINVGTEIKLFTRHSEIKNKAECLISIGGDGSIMDAVTIVKDSGIPILGINTGRLGFL
                     SNISLEQISTAIENLFEKEYRIEKRALLRVETSNNIFGSTNFALNELTIHRKDSGSMI
                     TIHAQVEDHYLNSYWADGIIIATPTGSTAYSLSAGGPIVEPGSNNFVITPIAPHNLNV
                     RPMIISDDNIIRLKVEGRSKHFLATLDSRSFNVDSQDELIIRKADFKIKLIKLQGQNF
                     YETIRNKLMWGIDKRN"
     gene            1304..>1522
                     /locus_tag="PK610_13810"
     CDS             1304..>1522
                     /locus_tag="PK610_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37067.1"
                     /translation="MKRIIVFLIIIICALPFSQNGYSQAWRYYRHEVRLGFGASNFLG
                     ELGGANNIGTNRMKDLEFAMTRPAVDVMY"
BASE COUNT          462 a          306 c          304 g          450 t
ORIGIN      
        1 acgtgagggg atacgagtat tatgttatcg acggacaaaa ctttgccatg tttcgatcga
       61 atgttaaata cagattgttt ggtccaaaaa ttcatgatct tgactttttg cagggaacga
      121 atttttcaaa acttcattat gccttttacc tcaatctgta tattgatgcc ggatacgttc
      181 aggaccggcg ctattacaca gaaaatccac ttgccaacca aatgctttat ggcacaggaa
      241 tcggactcga ttttgtaagc ttttacgata aagttattag atttgaatat tcgatgaaca
      301 aagagttgga gaaaggcttc ttcattcact tcatcaaacc tatatagcat gactatcgcc
      361 atttacggcc gttcattcag cgaagaacac attccgcaca ttcagcaact tttcgatgag
      421 cttgccaagc ggaaagcaca attgatcgtt ttcgaagctt tcaattcctt tttgcgccca
      481 agaattaatg tcggaacaga aataaaacta tttacccgtc attccgaaat taaaaacaaa
      541 gcggagtgtt tgatcagtat tggaggcgat ggctcaataa tggacgctgt tacaattgta
      601 aaagattcag gaatccctat tcttggtatc aacaccggtc gattgggatt tctttcgaac
      661 atctctttgg aacaaatcag caccgccatt gaaaatttat ttgaaaagga ataccgaatt
      721 gaaaaaagag cgctgttacg tgttgaaaca agcaacaaca tatttggaag taccaatttt
      781 gcactcaatg aattgactat tcaccgcaaa gattctggat cgatgattac catccatgcc
      841 caggtggaag atcactattt gaattcttat tgggcggacg gtattattat cgcaactcca
      901 acaggctcaa cagcctattc actaagtgca ggtggcccca ttgtagagcc gggttccaat
      961 aattttgtaa ttaccccgat tgcacctcac aacctaaatg tccgccccat gatcatttca
     1021 gatgacaata tcatccgatt gaaggtggaa ggtcgttcca aacatttcct ggccactctt
     1081 gattccaggt cgtttaacgt tgatagtcag gacgaattaa ttatccgtaa agccgatttc
     1141 aagatcaagc tgattaaatt acagggacag aatttttacg aaaccatccg caacaagcta
     1201 atgtggggta ttgataagcg gaattaacaa tttactgact ttggggtcaa agcaactaat
     1261 tggatttagc caattaatcc ctaaatttgt acccacaact tgcatgaaac gcataatcgt
     1321 atttttaatt atcatcatct gcgcactacc tttttctcaa aatgggtatt ctcaagcgtg
     1381 gaggtattac aggcatgagg ttcgcctggg ttttggtgca tccaattttt tgggtgagct
     1441 gggaggtgcg aataacattg gtacaaaccg aatgaaagat ttggagtttg ccatgactcg
     1501 tcctgcagta gatgtaatgt at
//
LOCUS       DAOMYG010000570         1520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_33310_length_1520_cov_7.700341, whole genome shotgun sequence.
ACCESSION   DAOMYG010000570 DAOMYG010000000
VERSION     DAOMYG010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33310_length_1520_cov_7.700341"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PK610_13815"
     CDS             <1..180
                     /locus_tag="PK610_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013751629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37068.1"
                     /translation="TDYWEYLFLPACFLFLGNILTVFKTNDPAKLDPELKKVALGTFL
                     FSILFFLGQYIKYSA"
     gene            177..800
                     /locus_tag="PK610_13820"
     CDS             177..800
                     /locus_tag="PK610_13820"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018674585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HRJ37069.1"
                     /translation="MKNKFSPYQLHPWHGIEIHSYSPSIVNAFIEIVPTDTVKYEIDK
                     VSGHLKVDRPQLFSNVVPALYGFIPKTYCGDQIAEFCMKKTGRKDIKGDGDPIDILVL
                     TEKEIPHGSILVQAIPIGGLRMIDKGEADDKIIAVLKDDVVFGKTTDIADLPQGLLDR
                     LRHYFLTYKAIPGENRQVVEIDAIYGAKEAREVIGCSITDYESLLSN"
     gene            complement(850..>1520)
                     /locus_tag="PK610_13825"
     CDS             complement(850..>1520)
                     /locus_tag="PK610_13825"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRJ37070.1"
                     /translation="DVERILSRCDSFTKLLFVCSPNNPTGNLIRSELIEELLERFPGM
                     VVVDEAYIDFCPEHSMAEMVLRFPNLVVMQTLSKAWGLAGIRLGIMIANADLVKVMNK
                     IKPPYNINSLTQETALKILADIPTMQQNVITLLEDRILLEKELQRLPVVSRVHPSDAN
                     FLLVRFVQPQEVFLYLIKKQIIVRDRSNVILCDDCLRITIGTPEENHALLEALKNYPL
                     QKIR"
BASE COUNT          438 a          328 c          314 g          440 t
ORIGIN      
        1 accgattatt gggaatacct gtttttaccc gcctgctttt tgtttttggg aaatattctc
       61 accgtattca aaacgaatga tcctgccaaa ctcgatcctg aactgaaaaa agttgcactg
      121 ggtacatttt tgttttccat tctatttttt cttggtcaat acattaaata ttccgcatga
      181 aaaacaaatt ctcaccctat caactacatc cctggcacgg catcgaaatt cattcatact
      241 caccttctat tgtgaatgcc tttattgaaa tcgttccaac cgatacggta aaatacgaga
      301 tcgataaagt atcgggacat ttgaaagtgg accgaccaca attgttttcc aatgtggttc
      361 ctgccttgta cggattcatt ccaaaaacat attgcggaga tcagatcgct gaattttgta
      421 tgaagaaaac cggaagaaaa gacatcaagg gcgatggtga tcctatcgac attctggtgc
      481 ttaccgaaaa agaaattcca cacggttcca ttctggtaca agcgattccc attgggggat
      541 tgcgcatgat cgacaagggt gaggccgacg ataagatcat tgctgttttg aaagacgatg
      601 ttgtatttgg aaaaacaact gatattgccg acttaccaca aggattactc gaccgtttgc
      661 ggcattattt cctgacctac aaagccattc ccggagaaaa ccgtcaggtg gtggagatcg
      721 atgcgattta cggcgctaag gaggcgagag aagtgattgg gtgtagtata accgattacg
      781 aatcactgct aagcaactaa tcttttcttc ttccgatgcc tttgattttt ttcatgattt
      841 tcttttcaat catcggatct tttgcaaagg ataatttttc agtgcttcca ataaagcgtg
      901 attctcctcc ggagttccta ttgtgatccg caggcaatcg tcgcacaata ttacattgga
      961 acgatcgcgg acgatgatct gttttttgat gaggtaaaga aaaacttcct gaggctgaac
     1021 aaagcgaacc aaaagaaaat ttgcgtcaga aggatgcaca cgacttacca caggcaatcg
     1081 ctgcaattcc ttctcgagta atatgcggtc ttcaagtaag gttattacat tttgctgcat
     1141 ggttggaata tccgcaagta tttttaaagc agtttcctga gtcaggctat tgatattgta
     1201 cggcggttta attttgttca tcaccttcac cagatcagca ttggcaatca tgattccaag
     1261 ccggatacct gcaagtcccc atgccttgga taaggtttgc atcaccacca gatttggaaa
     1321 gcgaagcacc atttctgcca tggaatgttc cggacaaaaa tcgatatacg cttcgtcaac
     1381 caccaccatt cccggaaaac gctccagtaa ttcttcgatg agttcagaac ggatcagatt
     1441 tccggtaggg ttatttggag agcaaacgaa cagtaatttg gtaaacgaat cacaacgcga
     1501 taagatacgc tctacatccg
//
LOCUS       DAOMYG010000571         1512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_33548_length_1512_cov_8.135896, whole genome shotgun sequence.
ACCESSION   DAOMYG010000571 DAOMYG010000000
VERSION     DAOMYG010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33548_length_1512_cov_8.135896"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="PK610_13830"
     CDS             <1..1351
                     /locus_tag="PK610_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRJ37071.1"
                     /translation="PFLFAGKDNAGEFYRKSFAGLFSYVSFRIPEISDEIYKVDDAMR
                     AGFGWSQGPFEIWDCIGVDNAAKAMKAAGVKYAAWIDDMIAAGIKSFYVIENGVRKYY
                     DIASKSYKTIPGTEQLLVLSNIRSSKTIWKNSGTTITDLGDGIINLEFHTKMNTIGGE
                     VIEGINKAIDLAEKNYRGLVISNEGDNFSAGANLGMVFMFAVEQEYDELDFAIRAFQN
                     AMMRVRYSSIPVVVAPHNMVLGGGCEMCLHADKVVAHAETYMGLVEFGVGVIPGGGGT
                     KEFAVRLSDELKDGDVELNQFRNRFLTIGQAKVSTSAQEAFELGYLRAGIDEVIVSRA
                     HQLTYAKEVCVALADKGYTQPSPRNDIRVMGNQAMGLVHVGANSMLSGKYISEHDEKI
                     SVKLGYVLAGGDLSVPTVVNEQYLLDLERKAFLELCGERKTLERMQSILTGGKILRN"
BASE COUNT          454 a          295 c          368 g          395 t
ORIGIN      
        1 tcctttcttg tttgcaggaa aagacaatgc aggagagttt tacagaaaaa gttttgcagg
       61 attgttttcc tatgtctcct tccgtattcc ggagatcagt gatgagatct acaaagtaga
      121 cgatgccatg cgtgcaggtt ttggctggtc gcaaggtccg tttgagattt gggattgcat
      181 tggagtagac aacgctgcaa aagcgatgaa agctgccgga gtaaaatacg cagcatggat
      241 cgacgacatg atcgctgcag gaataaaatc gttctatgta attgaaaacg gagttcgtaa
      301 atactacgac atcgcaagta aaagttacaa gactattccc ggcacagaac aattgctggt
      361 gctatccaat atacgcagca gcaaaacaat ctggaaaaac tcgggtacaa caattaccga
      421 ccttggtgat ggcattatca atttggaatt ccataccaag atgaacacca ttggaggtga
      481 agtcatagag ggaatcaata aagcgatcga tctcgcagaa aaaaattacc gcggactggt
      541 catctccaac gaaggcgaca atttttctgc cggtgcaaat ctgggaatgg tatttatgtt
      601 cgctgttgag caggaatacg atgaattgga ttttgccatt cgcgcatttc aaaatgcaat
      661 gatgcgggtt cgttattctt ctattccggt tgttgttgcc ccacacaata tggtgcttgg
      721 cggcggatgt gaaatgtgtc tccatgctga caaagtggtt gcgcatgcag aaacctatat
      781 gggattggtt gaatttggag taggcgttat tcccggaggc ggcggaacaa aagaatttgc
      841 agttcgtctt tccgacgaat taaaagacgg cgatgttgag ctcaatcaat tccgcaatcg
      901 attccttacg atcggtcagg ccaaagtttc aacttcagcg caggaagctt ttgagctcgg
      961 atatttgcgt gcaggtatcg atgaagtgat cgtaagcaga gctcaccagc tcacctatgc
     1021 aaaagaagtt tgcgttgcac ttgcagataa aggctacaca caaccatcac cacgaaatga
     1081 tatccgggta atgggtaatc aggcaatggg attggttcat gttggagcaa actcgatgct
     1141 gagtggaaaa tacataagtg aacatgatga aaaaatatcg gtgaaacttg gttatgttct
     1201 ggcaggtgga gatttgtcgg taccaactgt ggtgaatgaa caatatcttc tcgacctgga
     1261 aagaaaagca ttcctcgaac tatgcggcga acgcaaaaca cttgaacgca tgcaaagtat
     1321 tttaaccggt ggaaaaattc tgagaaacta agatgaatta cctggatctg ttcaagggat
     1381 tacaatataa aaatgtacgc tacctcattt gcggtggact ggccatgaat ttgtatggtg
     1441 ttccgcgaat gacagcagat atcgacctct tgcttgacct taacaaatcc aacaccgaca
     1501 acttttcatc ag
//
LOCUS       DAOMYG010000572         1480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_34694_length_1480_cov_10.351579, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000572 DAOMYG010000000
VERSION     DAOMYG010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34694_length_1480_cov_10.351579"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PK610_13835"
     CDS             complement(<1..256)
                     /locus_tag="PK610_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37072.1"
                     /translation="MSHKSSSDLYQLIKSMSRNEKRHFRQQADIYNREGEKAYMLVFN
                     AFDKSEDFNEDKIIKSLSLPAYPVVKSRLTQLLLRFLTNLH"
     gene            377..1399
                     /gene="ruvB"
                     /locus_tag="PK610_13840"
     CDS             377..1399
                     /gene="ruvB"
                     /locus_tag="PK610_13840"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRJ37073.1"
                     /translation="MSEEFDIREEDLPVDKDLDRVLRPKMFDDFTGQQKVIDNLSVFV
                     EAALQREEALDHVLLHGPPGLGKTTLANIIANELGVGIKITSGPVLDKPGDLAGLLTN
                     LEKGDVLFIDEIHRLSPVIEEYLYSAMEDYTIDIMIDTGPNARTVQITLNPFTLIGAT
                     TRSGLLTAPLRARFGINSRLEYYDAKTLTIIVERSAQILQIPIEEQAAYEIAFRSRGT
                     PRIANALLRRVRDFAQIKGDGTITFDIVQHSLDALNVDRHGLDEMDNKILRAIIEKFK
                     GGPVGLTTIATAVGEQAGTIEEVYEPYLIQEGYLIRTPRGRKATEMAYKHLGMNVPFF
                     RGGLFD"
BASE COUNT          448 a          311 c          310 g          411 t
ORIGIN      
        1 aatgcaaatt ggtcaaaaaa cgcaacaata gctgggttaa ccgagacttt acaacaggat
       61 aagctggtaa ggagagtgac tttatgattt tgtcttcgtt aaaatcctcc gacttatcaa
      121 aagcattaaa taccaacatg taggccttct cgccctcgcg gttgtaaata tcagcctgct
      181 gcctgaaatg ccttttctca tttcgagaca tgctcttgat taactggtaa agatcagaag
      241 agctcttgtg cgacatactg taaaaataga ggctcttgct ccggcatttt ttaacttagg
      301 gtaaaaaata tatcgaatat ttttctatcg cgtttcgcta ttaagggaag tgattatcct
      361 atctttgttt gtacccatga gtgaagaatt cgatattaga gaagaagatc tgccggtcga
      421 taaagatttg gatcgcgtct tgcgtcccaa aatgttcgat gattttaccg gacagcaaaa
      481 ggtcatcgat aatctctctg tttttgtaga ggccgcatta cagcgagaag aagccttgga
      541 tcatgtgttg ctccatggcc ctccaggttt aggtaaaacc acacttgcca atatcatcgc
      601 aaatgaactt ggcgttggaa taaagatcac atccggacca gtccttgaca aacccggtga
      661 tctcgcggga ttacttacca atcttgaaaa aggtgatgtg ttgttcattg acgaaataca
      721 tcgtttaagc ccagtcattg aagaatatct ttattcagca atggaggatt acacgatcga
      781 tatcatgatc gataccggac caaatgcgcg tacagttcaa atcacactca atcctttcac
      841 tcttattggg gcaacaacac ggtcaggact gttaaccgct ccattgcgtg caagatttgg
      901 aatcaattcc cgacttgaat actacgacgc aaaaacgctt acaataattg ttgaacgatc
      961 tgcacaaata ctgcaaatcc ccattgagga acaagctgcc tatgaaatag ctttcaggag
     1021 ccgtggcaca cctcgaattg caaacgcatt gcttcgtcgc gtacgtgatt ttgcgcagat
     1081 taaaggtgac ggaacaataa cttttgatat cgtccaacac tcactcgatg cgttgaatgt
     1141 agatcgtcat ggtttagatg aaatggataa taaaattctt cgcgcaataa ttgaaaaatt
     1201 caaaggcgga ccggttggac taacaaccat tgctactgcc gtgggcgaac aagcaggaac
     1261 gattgaagaa gtatacgaac cctacttaat ccaagaggga taccttattc gcacacccag
     1321 aggtcgcaag gcaactgaaa tggcatacaa acatcttggg atgaatgttc cattttttag
     1381 aggtgggctt ttcgactaaa tcaccgaatt gaacaaatca atacataccg atctgttccg
     1441 taaaacagct ttcgtggagg gattttcttt cgttggattt
//
LOCUS       DAOMYG010000573         1480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_34703_length_1480_cov_6.084211, whole genome shotgun sequence.
ACCESSION   DAOMYG010000573 DAOMYG010000000
VERSION     DAOMYG010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34703_length_1480_cov_6.084211"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..622)
                     /locus_tag="PK610_13845"
     CDS             complement(98..622)
                     /locus_tag="PK610_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37074.1"
                     /translation="MEYPKGHKHVVKELLDGKFVLPSEPLYQVVRENDEFYTQFFKAS
                     FDYELRTSAEYCFLISGESNEMLSRDICIFFGVLCYELDRDGRNFLDEIQFSEWDHER
                     LDRYFENSTYAELINSNKQLRDKDSRRTFVNSLSRRNIVEKHAEDKWTFTAAHKVFID
                     FASELAKNRIREGV"
     gene            complement(609..>1480)
                     /locus_tag="PK610_13850"
     CDS             complement(609..>1480)
                     /locus_tag="PK610_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37075.1"
                     /translation="GQINGAMGQLNQATQQNVRLFVDSIEKNTTKLLSETRALKSNLE
                     KIDYREKVHRASGWIEHYIIPLNEILDVNQTTSVTNQLHEVAYYVNIKRLDTTDELIR
                     GQFEKLYSFLVQTNDDLIRQSKLLTNELLPLIERIKTESVILTGWIEFLKKPYTARVP
                     KVFKTTMERAYNRSFYLNAKEYVEQFMDDEPVYFSDEPSFQEKWIFNRELYRVRMESQ
                     LPIDDFFGWCQNTVKKDFHLIDQEKFFSMVTLIFEDDLIMEADEKAEYTTIKASDVIM
                     KVPKVKIDRHGIS"
BASE COUNT          419 a          320 c          308 g          433 t
ORIGIN      
        1 ggataagtga tacctatttc ttgtgttggt tacggggtat ggaaaacaaa aaagtcccgc
       61 aaaagcggga ctctctgtca aaacaaaaaa tgcaagttta cacgccttcg cggatgcgat
      121 tcttggcgag ttcactggcg aaatcgataa acactttatg ggctgcagta aaggtccatt
      181 tgtcttctgc atgcttctca acaatatttc tacgcgaaag tgaattcaca aaagtgcggc
      241 gcgaatcttt atcacgcagc tgcttgttcg aattgatcaa ttcagcataa gtggaatttt
      301 caaaataacg atccagacgt tcatgatccc attcgctgaa ttgaatttca tcaaggaaat
      361 tccttccgtc gcgatcgagc tcataacaca acaccccaaa gaaaatacaa atgtcgcgcg
      421 acaacatctc attgctttca cctgaaatca ggaagcaata ttctgcagaa gtccgcaatt
      481 cataatcgaa actggctttg aagaactgcg tataaaactc atcgttttcc cgcacaacct
      541 gataaagggg ttctgaggga agaacaaatt ttccatcgag caattctttt acaacgtgct
      601 tatgtccttt aggatattcc atgacggtcg atttttactt tgggtacttt cataattaca
      661 tcggaagctt ttattgttgt gtattccgct ttctcatcag cttccataat gagatcgtct
      721 tcaaaaatga gtgtcaccat cgaaaagaat ttttcctgat cgatcaggtg aaaatccttt
      781 ttcacggtat tctggcacca gccgaagaaa tcgtcgattg gtaattgcga ttccatgcga
      841 acgcgataca actcacggtt gaaaatccat ttctcctgga agctgggctc atctgaaaaa
      901 taaaccggct catcgtccat gaattgttca acgtattcct tggcattaag atagaacgag
      961 cgattgtaag cgcgctccat tgtggtttta aatactttgg gaacgcgcgc ggtatagggt
     1021 tttttcagga attcaatcca gccggtaaga ataacagatt cggttttgat gcgttcgatc
     1081 aatggcaata gctcattggt aagcagtttc gactgacgaa tcaaatcatc gttggtctgc
     1141 accaaaaagg aatacaattt ttcaaactga ccacgaatca attcatcggt ggtatccagt
     1201 cgtttaatgt ttacgtagta cgcaacttca tgcaactgat tggtaaccga agtcgtttga
     1261 ttcacatcca gaatttcatt gagcggaata atataatgct caatccatcc ggaagcacgg
     1321 tgtacctttt cgcgataatc gattttttcg aggttcgatt tcagtgcgcg tgtttcagaa
     1381 agcagtttgg tcgtgttttt ctcaatggaa tccacaaaca agcgtacgtt ctgctgcgtc
     1441 gcctggttca actggcccat ggcgccgttg atctggccga
//
LOCUS       DAOMYG010000574         1463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_35332_length_1463_cov_10.978693, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000574 DAOMYG010000000
VERSION     DAOMYG010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35332_length_1463_cov_10.978693"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..>1463
                     /locus_tag="PK610_13855"
     CDS             182..>1463
                     /locus_tag="PK610_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37076.1"
                     /translation="MKTLVQDNKKLADSTGLDDQRHKRMDYTYDLISGNVHQVSFQKD
                     SVDAFHHKYEYDADNRITEVYTSRHHDPHPEATSVWENDAKYFYYAHGPLARVELGDL
                     KVQGMDYVYTLQGWIKGVNSSTLDSLRDPGLDGWVDNSNVNKYIPKDVMGYNLNYFTG
                     DYAAINTAKSGASAPFASIVGSDIESNRYDLYNGNIGSMVTTITNPTTREVLPLGNAY
                     QYDQLNRIKQARSFTNLDVANNIWQSGSTYSGKYENTFNYDANGNILGQLRKDHAGSV
                     IDSLTYRYARNTNNRIIRNRLYHVNDNTDYNSNDIDDMGTFTSSLSTINTANNYSYTA
                     IGELAKDEQEEIEEIQWTVSGKVKAIYRPDSSSKKELRFDYDINEQKIAFHVYSSSGQ
                     WEKSIYYVRDASGNVLSTYEHILIDSLEEISFRLS"
BASE COUNT          455 a          324 c          324 g          360 t
ORIGIN      
        1 gaatccaaaa aagaagaaaa gaataagata atttttcgtt agtttattta ggtaaaggaa
       61 tcttcggatt cctttttttt atatatttat tcctgaattc tcgttgggaa aattgataaa
      121 tacagctaaa tataattttt caggatgcac agggcattat tactacgaca tccacggcaa
      181 cgtgaaaacg ttggtgcagg ataacaagaa gttggcggac tcaaccgggc ttgatgatca
      241 aaggcataaa cgcatggatt atacctatga tctgatttcg ggcaatgtgc atcaggtttc
      301 tttccaaaag gattctgttg atgccttcca ccacaagtac gaatacgatg ccgataaccg
      361 catcactgag gtgtacacct cccgtcacca cgatccgcat cctgaggcga catccgtttg
      421 ggaaaatgat gcaaagtatt tttactacgc tcacggacct ctggcacggg tagaactcgg
      481 cgacctcaag gtacagggca tggattatgt gtacaccctt cagggatgga tcaagggcgt
      541 gaactcaagc accctggaca gcctgcgcga tcccggtttg gatggctggg tggacaacag
      601 caacgtgaac aaatacatcc ccaaggatgt gatgggctat aacctgaatt atttcacggg
      661 cgattatgca gctatcaata cggccaaatc cggggcatcg gcgccttttg ccagcattgt
      721 gggtagtgat attgaaagta accgctatga cctttacaat gggaatatcg gctcaatggt
      781 gaccaccatt accaacccca ccacccggga agtcttacca ttgggtaatg cctatcagta
      841 cgatcagctc aaccggataa aacaagcccg cagctttact aacctggatg ttgccaacaa
      901 catttggcaa agcggcagta cctattcagg caagtacgaa aacaccttta actacgatgc
      961 caacgggaat atcctggggc agctcagaaa ggatcatgcc ggcagcgtaa tcgatagctt
     1021 aacttaccgc tatgcccgca ataccaacaa ccgcatcatc cgcaacaggc tgtaccatgt
     1081 taacgacaat accgactaca acagtaacga tattgacgat atgggcacat ttacctccag
     1141 cctgtcaacc atcaatacgg ccaataatta cagctatacc gccattggtg aactcgccaa
     1201 agacgaacag gaagagattg aagagatcca atggacagta agcggaaaag tgaaggcgat
     1261 ttatcgtcca gattcgtcat caaaaaagga attaaggttt gattatgata taaatgagca
     1321 aaagattgct ttccatgttt attcaagttc aggacaatgg gaaaaatcaa tttattacgt
     1381 tagagatgcc agcggaaatg ttttgagcac atatgagcac atcttaattg actccttgga
     1441 agaaataagt tttcgtttaa gtg
//
LOCUS       DAOMYG010000575         1455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_35644_length_1455_cov_8.237857, whole genome shotgun sequence.
ACCESSION   DAOMYG010000575 DAOMYG010000000
VERSION     DAOMYG010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35644_length_1455_cov_8.237857"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..423)
                     /locus_tag="PK610_13860"
     CDS             complement(142..423)
                     /locus_tag="PK610_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRJ37077.1"
                     /translation="MLLFLNISGGEIVVIFLVVLLLFGPQSIPTIARTLGRGMRAIKD
                     ASSELQREIRESADRAQQQADIKGDIDLKQSLDITKELDITTEIEDPKL"
     gene            complement(507..>1455)
                     /gene="secF"
                     /locus_tag="PK610_13865"
     CDS             complement(507..>1455)
                     /gene="secF"
                     /locus_tag="PK610_13865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRJ37078.1"
                     /translation="TNTKFGFIKGRKLYYGISTILILGGIASLATKGLDYGVEFSGGY
                     AFEIQFQEEANYEKIRSNLSNVFVEDGQKLTPEVKKIENKFKAKVTTKFMINDTSSTA
                     ENTVTEKLKEGLIEWEGKYEILGSRKVGPVISGELIESSISAIIFALIIIFGYIAIRF
                     RKWQFGLAAVAALFHDALIVLGIFSIFYGILPFSLEIDQAFIAAILTVVGYSINDTVV
                     VFDRVREYLSENKRMETGSLIDNALNSTLSRTINTSMTTAVVLLAIFLFGGEAIKGFT
                     FALLVGVVVGTYSSLFIASPLVYDIAGKSIGNNTTTKKD"
BASE COUNT          446 a          296 c          293 g          420 t
ORIGIN      
        1 gggttgatct gaaaaaacat aaaaaaatgt gaacacaggt tgaaaaaatg gggggggtgt
       61 ccgaaaaaaa gatggtttta ttagtttcta ttcaaaataa aaggcccttt cgggcctgca
      121 ggtaggaatg gattgccgga attagagttt cggatcctcg atctctgtgg taatatccaa
      181 ttctttggtg atatccaggc tttgttttag atcgatatca ccttttatat cagcttgttg
      241 ttgtgctctg tcggccgatt cccgtatctc ccgttgtagt tctgaggacg catctttaat
      301 agcacgcata cctcggccaa gcgttctggc tatggtagga atactttgag gcccgaataa
      361 taaaaggacc accaaaaaga taaccacaat ttcacctccg gatatattca ggaacaatag
      421 catgatgcaa agttaagtgg aaatacgaca tccctaaaaa aagaaagccc cggaacttcc
      481 ggggctttgc tatttaactt aagtaattag tcttttttag tggtagtatt gttgccaata
      541 ctttttcctg cgatatcata taccaaagga gacgcaatga acaaggatga ataggttcct
      601 acaaccacac caacaagtaa tgcaaacgta aatcctttaa tggcttcgcc gccaaacagg
      661 aagattgcaa gcagtacaac tgcagtagtc attgaggtat taatagtacg acttagtgtg
      721 ctattaagcg cattgtcaat taatgagccc gtctccatgc gtttgttttc gcttaaatat
      781 tcacgtactc ggtcaaatac aacaacagta tcgttgatgg aataacccac tacagtaaga
      841 atcgctgcaa taaaggcttg gtcaatttcc aaagagaatg gtaggatgcc atagaaaatg
      901 gagaagattc ctaacacaat aagtgcatca tgaaagagcg cagcaactgc ggccaagcca
      961 aattgccatt tacggaatcg tattgcgata tacccgaaaa tgataatcag agcgaaaata
     1021 attgctgaaa tggatgattc aattaactcg cctgaaatta ccggaccaac ttttctggag
     1081 ccaagaattt catatttgcc ctcccactca ataagacctt ccttcaattt ttctgttacc
     1141 gtgttttctg cagttgaaga cgtatcgtta atcatgaact tcgttgtgac tttcgccttg
     1201 aacttgttct cgattttctt tacctcaggt gtaagcttct gaccatcctc aacaaaaacg
     1261 ttggagaggt tgctgcggat tttttcgtaa ttggcttctt cctgaaactg aatctcaaaa
     1321 gcataaccac cggagaattc aaccccgtaa tcaagtcctt tggttgcgag tgatgcgata
     1381 ccaccaagaa tcagaatagt tgaaattccg taatacagtt ttcttccttt aatgaatcca
     1441 aacttggtat tggta
//
LOCUS       DAOMYG010000576         1452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_35785_length_1452_cov_8.648533, whole genome shotgun sequence.
ACCESSION   DAOMYG010000576 DAOMYG010000000
VERSION     DAOMYG010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35785_length_1452_cov_8.648533"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..861
                     /locus_tag="PK610_13870"
     CDS             163..861
                     /locus_tag="PK610_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016649966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HRJ37079.1"
                     /translation="MNKKVADAHEAIRDIEDNMVLALGGFGLCGIPENCIAALVEKGV
                     KGLTCISNNAGVDDFGIGLMLQQKQVRKMISSYVGENAEFERQLLSGELEVDLIPQGT
                     LATRLMAAGYGMPVIFTPAGVGTEVAQGKEVRNFNGKDYLMEYAFDPAFSIVKAWKGD
                     THGNLVFRATSRNFNPLVAMAGKITIAEVEELVPAGELNPDHIHLPGIYVHRIFQGSS
                     YEKRIEQRTFRKKS"
     gene            880..>1452
                     /locus_tag="PK610_13875"
     CDS             880..>1452
                     /locus_tag="PK610_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HRJ37080.1"
                     /translation="MALDKNQIAQRIAREVRDGMYVNLGIGIPTLVANYIPDGVNVVL
                     QSENGLLGIGPFPFEGEEDPDLINAGKQTITTVPGSAFFDSALSFGMIRAGKVDLTIL
                     GAMEVADNGDIANWKIPGKMVKGMGGAMDLVASAKNIIVAMQHVNPKGESKLLPACTL
                     PITGVRCVKKIVTELAVLEVTPNDGFRLIER"
BASE COUNT          438 a          258 c          359 g          397 t
ORIGIN      
        1 aacgacctga tgatttccct gagcagtttt tgaattgtcg cgaaggaaaa gggatggcta
       61 gcatctggaa tgaaattaat tttcttgaag tggaaacaga tcaatacgaa cctgaaagta
      121 atggaggttc cattgaagac gataataaag aattcgaata acatgaataa aaaagttgca
      181 gatgcgcacg aggctattcg cgatattgag gacaatatgg ttcttgctct tggaggattt
      241 ggtttatgcg gaattccgga gaattgcatt gcagctctcg ttgaaaaggg cgtgaaagga
      301 ttaacctgta tttccaataa tgcaggagtg gatgattttg gaatcggact catgttgcag
      361 caaaagcaag tgcgcaaaat gatttcctcc tatgtgggag aaaacgcaga attcgaacgt
      421 cagctattga gtggtgagct ggaggtggat ttaattccgc aaggaacatt ggcaacacga
      481 ttgatggctg caggttatgg aatgcctgta atatttactc ctgccggtgt aggtacagaa
      541 gtagcacagg gaaaggaagt acgcaatttc aatggtaaag attatttgat ggagtatgct
      601 ttcgatcctg ctttttctat tgtgaaagca tggaaaggtg atacacacgg taatcttgtt
      661 tttcgtgcaa catcacgcaa ttttaatcca ctggttgcaa tggcaggaaa aattaccatc
      721 gccgaagtag aagaacttgt tcctgcaggc gaattgaatc ccgatcatat tcacttaccc
      781 ggtatttacg tacatcgaat ttttcagggt agcagttatg agaaacgaat tgagcaacgt
      841 actttccgca aaaagtcgta atacaaacac tttacaaaaa tggcactcga taagaatcaa
      901 atagcacaac gtattgcccg cgaagtccgc gatggtatgt acgtgaacct gggaatcgga
      961 atccctactt tggttgcaaa ttatattccg gatggagtaa atgttgtttt gcaatcagag
     1021 aacggattgt tggggattgg tccttttcca ttcgaaggcg aagaagatcc tgatctgatc
     1081 aatgcaggta aacaaaccat tacaactgtt ccgggatctg ccttttttga ttcagcgctt
     1141 agtttcggaa tgatccgtgc aggtaaagtg gatctcacca ttttgggtgc aatggaagtt
     1201 gccgataatg gagatattgc aaactggaag atacccggga aaatggtgaa ggggatgggt
     1261 ggagcaatgg atctggttgc ttctgcaaaa aacatcatcg ttgcaatgca acacgtcaat
     1321 cctaaaggag aatctaaatt attaccggcc tgtacactcc cgattaccgg cgttcgctgc
     1381 gtaaagaaaa ttgtaactga acttgccgta ctggaggtaa ctcccaatga tggttttcga
     1441 ttgattgaac gc
//
LOCUS       DAOMYG010000577         1452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_35791_length_1452_cov_7.068719, whole genome shotgun sequence.
ACCESSION   DAOMYG010000577 DAOMYG010000000
VERSION     DAOMYG010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35791_length_1452_cov_7.068719"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..600)
                     /locus_tag="PK610_13880"
     CDS             complement(151..600)
                     /locus_tag="PK610_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37081.1"
                     /translation="MKKVFQFPANAELQMRRDRLIIFLFIICGLFAVYVVIGNIVNER
                     ESSAAMLISIVALVVFALLAWLIRTRFGLITEVRIHEETIEIIRGKNQHSQIEIRKIV
                     ALLYYPQGKKSYYAIQHLSPDLLVIENAGQLFDAISEQTGIVLTEVK"
     gene            complement(597..1076)
                     /gene="rnhA"
                     /locus_tag="PK610_13885"
     CDS             complement(597..1076)
                     /gene="rnhA"
                     /locus_tag="PK610_13885"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018674956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HRJ37082.1"
                     /translation="MKVHIFTDGAAKGNPGPGGFGIVLRSERGHHKEVARGYRLTTNN
                     RMELLAVIVALEMLKGSQDEVTVFSDSRYVVDSVEQGWLWNWQRKGFEKTKNADLWRR
                     FIPAYKRHKVKFQWIKGHAGHKENERCDQMAVAAADLPVLLVDEEYEKLKDTGMFEE"
     gene            complement(1076..>1452)
                     /locus_tag="PK610_13890"
     CDS             complement(1076..>1452)
                     /locus_tag="PK610_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRJ37083.1"
                     /translation="NAFAVAGSFVLFLMGIEMILGVRLFREEEGNAKAASIVPLAFPI
                     IAGAGTMTTILSLRAEFEAINIIIAIVINSLFVYMVLRLTNRIERLLGPGGLIVLRKV
                     FGIICLALAVKLFSSNIKELFV"
BASE COUNT          442 a          335 c          267 g          408 t
ORIGIN      
        1 atttttaacc gttggggaca aaccattttt gaaaaatcga attatcaaaa tgactggaaa
       61 gcggaaggtt atccggatgg ggtttacttt tttgtattaa cggcaggcga tcaaaaatac
      121 aacggaaccg tgcatgtaat ccgacactga ttattttacc tccgttaaca cgattcctgt
      181 ttgttctgaa atcgcatcaa ataattggcc tgcgttttca atgacaagaa gatcgggact
      241 taaatgctga atagcgtaat aggatttttt tccctgagga tagtataata aggcaactat
      301 cttcctgatt tcaatctgtg agtgctgatt tttaccccga ataatttcaa tagtttcttc
      361 gtggatccgt acttcagtga tcaaaccaaa gcgcgtccgt atcaaccacg caagcagggc
      421 aaaaacaacc agcgcaacaa tggagatcaa catggctgct gatgattccc tttcgtttac
      481 tatgttgcct ataacgacat aaacagcaaa caaaccacaa atgatgaaga ggaaaatgat
      541 cagacgatct cttctcatct gaagctcagc attggcagga aactgaaata cttttttcat
      601 tcttcgaaca ttccggtgtc cttcaatttt tcatattcct catcaacaag caaaacagga
      661 agatcggcag cagcgacagc catctgatca cagcgttcgt tttccttgtg acctgcatgg
      721 cctttgatcc attgaaattt tactttatgt cgtttgtatg ctggaataaa tcgtcgccac
      781 aaatcagcat tctttgtctt ctcaaatcct ttacgttgcc agttccacaa ccatccttgt
      841 tccactgaat ctaccacata tcgtgaatca gaaaacacgg taacctcatc ctgacttccc
      901 ttcagcattt ctagcgctac aataacagca agcaactcca tccggttatt tgtggtgagg
      961 cgatatccgc gtgctacttc cttgtggtgt ccgcgctccg atcgcaaaac gattccgaaa
     1021 ccaccgggcc ccggatttcc ttttgctgcg ccgtcggtaa aaatatgaac cttcatcaga
     1081 caaacaattc tttgatgttg gaactaaaaa gttttacagc aagcgcaaga cagatgattc
     1141 cgaaaacctt tcgcaataca attaatccac ccggacccaa taatcgttca atacgattgg
     1201 taagacgaag caccatgtaa acaaacaaac tattgataac aatagcgatg atgatattga
     1261 ttgcttcaaa ctcggcacgc agagataaaa tagtggtcat ggtgcccgca cctgcaataa
     1321 tgggaaatgc gagaggaacg atagaagctg cttttgcatt tccttcttct tctcgaaata
     1381 atcgtacacc taatatcatt tcaattccca ttaaaaacaa aacgaaggat cctgctactg
     1441 caaatgcgtt ta
//
LOCUS       DAOMYG010000578         1443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_36110_length_1443_cov_6.956772, whole genome shotgun sequence.
ACCESSION   DAOMYG010000578 DAOMYG010000000
VERSION     DAOMYG010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36110_length_1443_cov_6.956772"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..1024)
                     /locus_tag="PK610_13895"
     CDS             complement(71..1024)
                     /locus_tag="PK610_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013620976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HRJ37084.1"
                     /translation="MAKAITIGKESVAPGESKIINYDIARLPSGMMINMPVFVYRSKV
                     EGPVLLLSGGLHGDEVNGIEIIRRMTSEGMFNKLIAGSIIAIPVLNVFGFLNYSREVP
                     DGKDVNRSFPGDSNGSLASRVAWHLTKNILPLIDYGVDFHTGGAQRTNYPQMRYSLVD
                     KNSELIAKAFAAPFTLNSGLIPKSIRHQGSKMGKPIIVFEGGESMRFDELSISEAIDG
                     TKRLLCYLGMINEKYEERKGILLMDSSWIRANRAGLFKPFVDSGDPVQKNQPVGVIND
                     PFGQYEIKVLSKYSGHIIGMNNNPVVHQGDALFHVGLEEGR"
     gene            1146..>1443
                     /locus_tag="PK610_13900"
     CDS             1146..>1443
                     /locus_tag="PK610_13900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HRJ37085.1"
                     /translation="MIHPGTELRFISNEVGNGVFATTFIPKGTIVYIKDKLEIVIPPD
                     DPRRNDPLYKDVIEKYSYTEPNGNFVLSWDIGRYVNHCCNCNIISTGYGFEIAIR"
BASE COUNT          401 a          312 c          299 g          431 t
ORIGIN      
        1 ttctttaaac ctgatttttt tgagaccatc tattgtctga aaaacaatta aggctttatt
       61 taacaggaat ttatctcccc tcttccaatc ctacatgaaa caatgcatct ccctggtgga
      121 ctacgggatt attgttcatt ccgatgatat gtccgctgta tttggaaagg actttaattt
      181 catactgtcc aaaaggatcg ttgataaccc caacaggttg atttttttga acgggatcac
      241 cggaatccac aaatggttta aataatcctg cgcggttggc tctgatccag gagctatcca
      301 taaggagaat tccttttcgc tcttcgtatt tctcgttgat cattcccaaa tagcaaagta
      361 aacgcttggt gccatcgatt gcttcgctta tggataactc atcaaagcgc atggattctc
      421 ctccttcgaa gacgataatt ggtttcccca ttttcgatcc ctggtggcgg attgatttgg
      481 gaattaatcc agaattgagg gtgaaaggag cagcaaaggc ttttgcaatg agctcggaat
      541 ttttatcgac cagtgaatag cgcatttgcg gataattggt acgttgtgct ccaccggtat
      601 gaaaatccac tccgtaatcg atgagtggta aaatgttttt tgtgagatgc cacgcaacac
      661 gacttgccaa ggaaccattc gaatcgccgg ggaaactgcg attcacatcc ttcccatcgg
      721 gaacttcacg cgaatagttc aggaatccaa aaacattgag caccggaatg gcaataatag
      781 aaccggcaat cagcttgtta aacatacctt ctgaagtcat tctgcgaata atctcgattc
      841 cattcacctc atcgccatgt aatccaccag aaagcagtaa aacaggtcct tcgaccttgc
      901 ttcggtaaac gaacacgggc atattgatca tcattccgga tggtagacgt gcgatatcgt
      961 aattgataat cttgctttcg cccggagcta ccgattcttt tccgattgtg attgccttag
     1021 ccatgcttcc attttttata aaagtacaac tccttttgag caagggtcag gattggaaag
     1081 aaaatttttg gctttctaaa aaagtaatcg ttcttccatt taatttggtg gcgatataag
     1141 gacctatgat ccaccccggc actgagctcc gctttattag taacgaagtt ggaaacggcg
     1201 ttttcgccac tacgtttatt cccaagggaa caatcgtata tattaaggac aaattagaga
     1261 ttgtaattcc tcctgatgat ccgcgaagga atgatccgct ttacaaggat gtgattgaaa
     1321 aatattcata caccgaaccc aatgggaatt ttgtgctgag ctgggatatt ggccgatatg
     1381 ttaaccactg ttgcaactgc aatatcatta gcaccggata cggttttgag attgctattc
     1441 gcg
//
LOCUS       DAOMYG010000579         1434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_36437_length_1434_cov_7.551124, whole genome shotgun sequence.
ACCESSION   DAOMYG010000579 DAOMYG010000000
VERSION     DAOMYG010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36437_length_1434_cov_7.551124"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            403..624
                     /locus_tag="PK610_13905"
     CDS             403..624
                     /locus_tag="PK610_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37086.1"
                     /translation="MYLFFKQTQSFRALVLRAGVSYTAVLLIFFFVYNSLHYEEYNAM
                     QWFYDCTLPGFLLSSASVIACLIPNKKKQ"
     gene            720..1379
                     /locus_tag="PK610_13910"
     CDS             720..1379
                     /locus_tag="PK610_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37087.1"
                     /translation="MKHFLNLSSFLFIFSLILMLSCRNAPTTESSIGSDSTQAAADTI
                     IEEKEAQVLTAFDEYLSLFPGATIPLVILNPDSLNSYKPDNDATLFSSFRGTTFFNSQ
                     WAACKRIPSPENTVAVLTVENGLAYNKVLLLETYTPEGIPIDKKYLDFGFEENGDPAG
                     SVEFKKDFSFHVFTKSEGVEYDENNNEIPGSQQVHKHSMQGKIQNDGKIQLSQKIWIN
                     K"
BASE COUNT          436 a          331 c          238 g          429 t
ORIGIN      
        1 tatagaagag agtagttaaa tttgatctta tcgaaatgca tttgaaggtt ttcctcttta
       61 ttgttatttt attcagtagt tgtcccaatt atcccaatta tccgacctaa atgaacaatg
      121 gggtggcaaa ctgatcaaag accaatccga agtgtttttg gatttttcct ggatctcctt
      181 tgcttggctt accttaattt ttgtacatta ccctaataaa tacgtcgaaa ttaattgtga
      241 caaccggcat tgaaaactaa aaaaaactac atccttttca ttctctacct actgctaaat
      301 ctgttgatgt ttggctacgc atcatccttt ttgcagtgca cagaattttt atgttatcac
      361 ctcccattga ttgcggtatg gactgtcttt ggaacttcaa ccttgtatct ttttttcaag
      421 cagacacaat cctttcgagc attagttctt cgtgccggtg tttcgtatac agcggttttg
      481 ctcatctttt tctttgtgta caattccctt cattatgaag aatacaacgc aatgcaatgg
      541 ttttacgatt gcactttacc cggcttcctg ctaagcagtg ccagcgtaat tgcctgtttg
      601 attcccaata agaaaaagca ataaattaag ataaattcca aagtataacg gaaataaata
      661 attcagtttg caaatagagt catcgattca aatttagtaa tccgcataga tattttttta
      721 tgaagcactt ccttaacctt tcatccttcc tgttcatttt ctccctcatc ttaatgctct
      781 cctgccggaa cgcccctaca accgaatcaa gtatcgggag cgattccacg caagcagctg
      841 cagacaccat tatcgaagaa aaagaggcac aggtactaac agcattcgat gaatacctgt
      901 ctcttttccc cggcgcaact atcccgcttg ttatcctcaa tcccgatagc ctcaactcat
      961 ataaacccga taatgacgcc actctgttca gttcattcag gggtactact tttttcaatt
     1021 ctcaatgggc agcctgcaag cgaattccct cacctgaaaa tacagtagcc gtactcaccg
     1081 tcgaaaacgg attagcgtac aataaggtac ttctgcttga aacttacaca cccgaaggaa
     1141 tccccatcga taaaaagtac ctcgatttcg gattcgaaga aaacggcgat cctgccggtt
     1201 cagtagaatt caaaaaggat tttagtttcc acgttttcac caaaagcgaa ggtgtggaat
     1261 acgatgaaaa caacaacgaa attccgggtt cgcaacaagt gcacaaacac tcaatgcaag
     1321 gaaaaatcca gaacgatggt aaaattcagc tcagtcaaaa aatctggatc aacaaataac
     1381 ggcccccacc tcaatcaccc ttcagaataa catatttaaa gtggtaaaca aatc
//
LOCUS       DAOMYG010000580         1425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_36792_length_1425_cov_6.963504, whole genome shotgun sequence.
ACCESSION   DAOMYG010000580 DAOMYG010000000
VERSION     DAOMYG010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36792_length_1425_cov_6.963504"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PK610_13915"
     CDS             <1..514
                     /locus_tag="PK610_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HRJ37088.1"
                     /translation="VFQDPSLEDMAVQYPINIKELNNITGVGPGKAERFGQPFVDLIS
                     RYVEENNIERPDDLVIRSVANKSAAKVQIIQNIDRRLPLEAIGKAQGKNVDEVITELE
                     TIVSSGTRVNINYVIEDLLDEDNQEEIFDYFMGADSDSIVDAYKEFDGAYSEEELRLM
                     RIKFMSEIAN"
     gene            582..926
                     /locus_tag="PK610_13920"
     CDS             582..926
                     /locus_tag="PK610_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37089.1"
                     /translation="MRKVLFSFVALLFLAACGGGPKIEGSWSLEAVSGEELSESEKAM
                     VFTFNEDGSLVMSNGEREEKGSWAWSEDKKDIILKNPNRDGEEKMEGVTIDGDVVKFK
                     SRSDEITLKRKK"
     gene            complement(1356..1425)
                     /locus_tag="PK610_13925"
     tRNA            complement(1356..1425)
                     /locus_tag="PK610_13925"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1398..1400),aa:Leu,seq:taa)
BASE COUNT          427 a          260 c          350 g          388 t
ORIGIN      
        1 ggttttccag gatccttcat tggaggacat ggcggtacag tatccgataa atattaaaga
       61 actcaataac atcaccggtg tggggcctgg gaaagcggag cgctttggtc agccttttgt
      121 cgatctgatt tcccgatacg tggaagagaa caatattgag cgtccggatg atcttgtaat
      181 tcgatccgtt gccaataagt cggcagcaaa ggttcagata attcagaata ttgaccgtag
      241 attacctctt gaggcaattg gaaaggcgca aggaaagaat gttgatgagg tgatcactga
      301 gctggaaaca attgtctcat cgggaacccg ggtaaacatc aattacgtaa ttgaggattt
      361 gctggatgaa gacaaccagg aagaaatatt cgactacttt atgggtgctg attcggattc
      421 aattgttgat gcttacaagg agtttgacgg tgcttactcg gaagaggaat tacggttgat
      481 gcgaataaaa ttcatgagcg agattgcaaa ttagtggatt gggtttgttt ttattgtttg
      541 gattggttta tctttaacca cctaaactaa aacctaaaat catgagaaaa gtattgtttt
      601 cttttgtcgc tcttctgttc cttgcagcat gtggcggcgg accaaaaatc gaaggaagct
      661 ggagtcttga agcggtatcc ggagaagaat tatccgagtc tgaaaaggct atggtattta
      721 cattcaatga agatggatca ttggtaatga gcaatggcga gcgcgaagaa aaaggcagct
      781 gggcatggtc tgaagataaa aaagacatca tcttgaagaa cccaaaccgt gatggagaag
      841 agaaaatgga aggtgttaca atcgacggcg atgtagtgaa gttcaagtca cgcagcgacg
      901 aaattacgct taagagaaag aagtaatttt cattttcagt ataggaaaaa cccggagtta
      961 tccgggtttt tttgttttta atggttcgaa ttccggttca tagttattgt tcatatggct
     1021 ttgaatttat cccagataat aatacaatat agatagagta tcccggatcg tcttgggatg
     1081 ctttagacca gaatctgccg gcgtcagtgt tcaaaggaaa agcccgcctg aataacctta
     1141 gccctcccat ccctcctgaa cgaattcagt cgggcaggct tgccaggatg acatgagtca
     1201 ggctgggaac cgttacggat aatcgaagta gatgaagata tggtagccaa cactgaattt
     1261 tttccgaaaa tccgtttaca ggaagtgaaa ggtcattacg aaataaaaaa ggcttgaaga
     1321 ttcatcttca aacctttcta cttcactgta cccgaggccg gactcgaacc agcacggaca
     1381 tttctgccca agggatttta agtccctcgt gtctaccatt tcacc
//
LOCUS       DAOMYG010000581         1423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_36866_length_1423_cov_6.009503, whole genome shotgun sequence.
ACCESSION   DAOMYG010000581 DAOMYG010000000
VERSION     DAOMYG010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36866_length_1423_cov_6.009503"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1423
                     /locus_tag="PK610_13930"
     CDS             <1..>1423
                     /locus_tag="PK610_13930"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRJ37090.1"
                     /translation="LMRFCFALLLLLFFSPAKAQVGEETRKAQILEQTIELIGESLGQ
                     EDIDLTTVFDQLSYYYDNPINLNKTSREELQELYLLSEIQITNLLMHIERNGKFLSIY
                     ELQAVPGFDLQIIRAILPFVKIADRFDAPHADWKTLMRDGQNELFLRWTRTLEPQSGF
                     APISDSLLALKPNSRYLGSPDRLYMRYRYRFGNKISFGITGEKDPGEEFFRGTQKNGF
                     DFYSAHFLIRDIGPLKTLAIGDYQVSFGQGLTFATGLALGKTANSLNVKRTSTTIRPY
                     TSVDENQFMRGAAATMKFGKMEFTGFVSRNRVDANRILVEDTSQVNDEGFVVSSLQTS
                     GIHATPAELEDKGALLLSHYGGHTAWKTKRTNIGATAVYSTLSTALEKNITYLNQFDF
                     TGKSNLVTGLDFNHIWRNFNFFGEFSRSVNGGMAMQSGILASLDPKFSLVLFYRNYQK
                     NYQSLFANALAEGSRPVNEKGLYI"
BASE COUNT          422 a          331 c          292 g          378 t
ORIGIN      
        1 cctaatgagg ttttgtttcg ctttactcct attgcttttc ttctctcccg caaaagcgca
       61 ggtaggagaa gaaacacgta aagcacaaat ccttgaacaa accatcgaac ttattggtga
      121 atcactcgga caggaagata tcgatctcac taccgtattt gatcagctga gttattatta
      181 tgataatcca atcaacctca acaaaacttc gcgcgaagaa ttgcaggaac tgtatttgct
      241 gagcgaaatt caaatcacca acctgctgat gcacatcgaa cgcaacggca aattcctttc
      301 tatttacgag ttgcaggcgg tacccggatt tgatttgcag atcatccgcg ccattcttcc
      361 ctttgtgaaa attgcagatc gtttcgatgc accacatgcc gattggaaaa cactgatgag
      421 agatggacaa aacgaattgt ttctccgctg gacacgcacg cttgagcctc aatcgggatt
      481 tgctcccatc agcgatagtc tgcttgcact aaaacccaat tcgcgctacc tgggtagtcc
      541 ggatcgattg tatatgcggt accgttatcg ttttggaaac aaaatttcat ttggtattac
      601 aggagaaaaa gatccgggag aggaattctt tagaggcaca caaaaaaatg gttttgattt
      661 ttacagtgcg cattttctaa tacgcgatat tggtccgctc aaaacacttg caattggcga
      721 ctatcaggtt tctttcggac agggcctcac cttcgctacc ggacttgcac tgggaaaaac
      781 cgcgaactca cttaacgtaa aaagaaccag cactacaatt cgcccctaca cctcggtcga
      841 tgaaaaccaa tttatgcgtg gagctgcagc aacaatgaaa ttcggtaaaa tggaattcac
      901 cggatttgtt tcacgcaaca gggtagatgc aaaccgtatt ctggttgaag atacatcgca
      961 ggtaaatgat gaaggatttg tggtttcatc gctgcaaaca tccggcatac atgctacacc
     1021 tgcggaactc gaagataaag gcgctttact attgagtcat tacggaggac acaccgcctg
     1081 gaaaacaaaa cgaaccaaca tcggtgctac tgccgtttat tcgacgcttt ctacggcatt
     1141 ggaaaaaaac atcacctacc tcaaccaatt tgattttacc ggaaaaagca atcttgtaac
     1201 cggacttgat ttcaatcaca tctggagaaa ctttaatttt ttcggcgaat tctcccgcag
     1261 tgtcaatggt ggaatggcaa tgcaatcggg aattcttgct tcactcgatc cgaaattttc
     1321 actcgtgttg ttctaccgca attaccagaa aaattaccag tcacttttcg ccaacgcact
     1381 tgcagaagga agtcgacctg tgaatgaaaa aggattatac atc
//
LOCUS       DAOMYG010000582         1422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_36896_length_1422_cov_8.927579, whole genome shotgun sequence.
ACCESSION   DAOMYG010000582 DAOMYG010000000
VERSION     DAOMYG010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36896_length_1422_cov_8.927579"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PK610_13935"
     CDS             complement(<1..455)
                     /locus_tag="PK610_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HRJ37091.1"
                     /translation="MRHLIFLFVLLCAGHASFAQIGGSTGSSAFNLVSSARVASLGGN
                     LIAVKDNDPSLGIHNPALLNKSMDRYLSMSYVNYFGNINYGFLSYVHDVDTLATFSAT
                     LNYLSYGRFKETDETGQELGEFSAGDYVLNLGAGRRIDSLFSVGLNLKTL"
     gene            587..>1422
                     /locus_tag="PK610_13940"
     CDS             587..>1422
                     /locus_tag="PK610_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019038127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ37092.1"
                     /translation="MSEQTEHVSCLIIGSGPAGYTAAIYAARAELKPVMYQGLQPGGQ
                     LTITTEVENFPGYPEGVQGPEMMVQLQQQAERFGTQVRYGLATKVDFTGPVHKVWIDD
                     EKLITADTVIVSTGASAKWLGLPSEQRLNGFGVSACAVCDGFFYRGQDVAIVGAGDTA
                     AEEATYLAKLCTKVYMLVRKSEMKASKAMQTRVLNTPNIEVLFDTETDEVIGEKTVEA
                     VRVKNSKTGTMRDLKVTGFFVAIGHEPNTGIFKGWLDMDETGYLKVVPGTTKTNIPGV
                     FAA"
BASE COUNT          440 a          301 c          314 g          367 t
ORIGIN      
        1 agtgttttga gattgagtcc aactgaaaaa agtgaatcga tccttcgacc ggcacccaga
       61 ttcaacacat aatctccggc cgaaaattct cccagttcct gtcctgtttc atcggtttct
      121 ttaaatctgc cgtagcttaa gtaatttaaa gtagcactaa aggttgcaag cgtatccaca
      181 tcatgcacat aggataaaaa cccgtagttg atgtttccga aatagtttac atacgacatc
      241 gacaggtaac ggtccatcga tttattcaac aatgcaggat tatgaattcc aagtgaagga
      301 tcattatcct ttactgcaat cagattacct cccaaactgg caactctggc actactaacc
      361 aggttaaatg ccgagctgcc ggtacttccg ccaatctgcg caaagcttgc gtgaccggca
      421 caaagtagca caaagagaaa aatcaggtgt cgcataagaa gattaacgca agataagcaa
      481 acttattact gaacatcagg ttcggaaaaa atccaatttt aggtgacatt cttcacaacc
      541 aagcgctgcc ttatttgtta cttttgccac acaaacaaac aatttcatga gtgaacaaac
      601 agaacatgta agttgcctga ttatcggatc gggtcctgcc ggatataccg ctgccattta
      661 cgctgcacgt gccgaattga aaccggtcat gtatcaggga ttgcaaccgg gcggacagtt
      721 aaccattacc accgaagtag aaaacttccc aggctatccg gaaggagtac aagggccgga
      781 aatgatggtt caattacaac aacaagctga gcgttttgga acgcaggtgc gttatggttt
      841 ggcaactaaa gttgatttca ccggaccggt tcacaaagta tggatcgacg atgagaaatt
      901 gattactgct gataccgtaa ttgtttctac gggtgcatct gccaaatggc tgggattacc
      961 ttctgaacaa agacttaatg gatttggagt ttcagcctgt gctgtttgcg atggattctt
     1021 ttatcgcggt caggatgttg ccattgttgg tgccggagat accgctgcag aagaagcaac
     1081 ctatcttgct aagctgtgca caaaagttta catgctcgtt cgtaaaagcg aaatgaaagc
     1141 atcaaaagca atgcaaaccc gcgtattgaa tacaccaaat attgaagtat tatttgatac
     1201 agaaacggat gaagttattg gtgaaaaaac cgttgaagct gttcgcgtaa aaaacagcaa
     1261 aaccggaacc atgcgtgatt tgaaagtgac cggatttttt gttgcaatcg gacatgaacc
     1321 caataccgga atttttaaag gctggttgga tatggacgaa accggatacc tgaaagttgt
     1381 accgggtaca actaagacca atattccagg ggtatttgct gc
//
LOCUS       DAOMYG010000583         1404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_37639_length_1404_cov_9.449963, whole genome shotgun sequence.
ACCESSION   DAOMYG010000583 DAOMYG010000000
VERSION     DAOMYG010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37639_length_1404_cov_9.449963"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..474)
                     /locus_tag="PK610_13945"
     CDS             complement(82..474)
                     /locus_tag="PK610_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37093.1"
                     /translation="MKKYFLIPVLFAGLLASCGEKSKKGAWIQADKDKFVSECMKGGE
                     GESIPGMDAAAMDKFKKDVCDCAVVKAEAEYENFEGANGDEPGMTKIGEACGTEIAMK
                     MLEDLMKNMPEEVTTGDDTTVTTEEVVE"
     gene            610..>1404
                     /locus_tag="PK610_13950"
     CDS             610..>1404
                     /locus_tag="PK610_13950"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ37094.1"
                     /translation="MSRLLLSVSLLALILISCGGDRKDSAEISGKKVFRYNESAGISF
                     LDPALAVRFEDGWAMSQLYNGLVTLDNDLNVVPDIARKWTVTEDKLEYTFTLRNDVYF
                     HDHPAFPEGKGRKVVAQDFVNSFFRVTDPDVASPGQYIFQNLDKSSRSFGLGFFAPND
                     STLKIVLAKPQQAFLEQLTLSFCYVIPIEIVDYYGDDFSRNPVGTGPFRFKSWKEDVK
                     LVMLKNENYFEKDENGNQLPYIDAISISFIREKDVEFREFLKGKYEF"
BASE COUNT          392 a          328 c          272 g          412 t
ORIGIN      
        1 ggttgttgaa taattgcatt tctgaaaatg aagaagaaag gggaccgtag ccccctttct
       61 tcttcatttt cagaaatgca attattcaac aacctcttca gtagtaacag tggtatcgtc
      121 accggtagtt acttcttcag gcatgttttt cataagatct tcgagcatct tcatagcaat
      181 ctcagttccg caagcttcac cgattttggt cattccgggc tcatcgccgt ttgcaccttc
      241 aaagttctca tattcagctt ctgccttaac aacagcgcag tcgcaaacat ctttcttgaa
      301 cttatccatg gctgcagcat ccattccggg aatagattcg ccttcgccac ctttcataca
      361 ttcagaaaca aatttgtcct tgtcggcctg aatccaggca cctttcttac ttttttcccc
      421 acaggaagcg agaagacctg caaacagaac ggggataagg aaatactttt tcatggtttt
      481 tttggatttg agtgcaatat aggcaaaatc ccatttcagc aaaaaaacac gacgaattcg
      541 tcttatttcc gctccctttt tcctgtcttt atccaacagg gagattatct ttgaggcaaa
      601 caaaatttca tgtcccgact cctcctatcg gtatctctat tggcactcat cctgatttcc
      661 tgcggcggcg accgtaaaga ttcagcagaa ataagcggaa aaaaagtatt tcgctacaac
      721 gaatcagcag gaatttcctt cctcgatccc gctttggcag tgcgctttga agacggctgg
      781 gcaatgagtc agctttacaa cggactcgtt acgctcgata atgatctgaa cgttgttccg
      841 gatatcgcca gaaaatggac cgttaccgag gacaaactgg aatatacctt tacgctgcgc
      901 aatgatgtgt atttccacga tcacccggca tttccggaag gcaaaggaag aaaagtggtt
      961 gcgcaggatt tcgtaaacag ttttttccgc gtcaccgatc ccgatgttgc atcacccggt
     1021 cagtatattt ttcaaaacct cgataaatct tcccgctcat tcggactcgg cttttttgca
     1081 cctaacgatt ctacactgaa aattgtactg gccaaaccac aacaagcatt tcttgaacag
     1141 cttacacttt ccttttgcta tgtaattccc attgaaattg tggattatta cggtgatgat
     1201 ttttcaagaa accctgtggg taccggacca ttccgattca aatcatggaa agaagatgtg
     1261 aagttggtga tgcttaaaaa cgaaaactat ttcgaaaaag atgaaaacgg aaatcagctc
     1321 ccctatatcg atgccatttc catttcgttt attcgagaaa aagatgttga attcagggaa
     1381 ttcctgaaag ggaaatatga attc
//
LOCUS       DAOMYG010000584         1397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_37948_length_1397_cov_7.362891, whole genome shotgun sequence.
ACCESSION   DAOMYG010000584 DAOMYG010000000
VERSION     DAOMYG010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37948_length_1397_cov_7.362891"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(335..1003)
                     /locus_tag="PK610_13955"
     CDS             complement(335..1003)
                     /locus_tag="PK610_13955"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRJ37095.1"
                     /translation="MLTQDLISENIPPIKMSDTGNKALKWMDEFKVAHLPVVDGGKYL
                     GLISDADILDLNQPDKALGEQEDLELDDIFISENQHIYDAIKLMNENRLSIIPVLDVN
                     GNYMGAISMTNLMDKIGSFSSITEPGSVLVLEVSNQDYSLSQIAQIVEGNDAKILSVF
                     LNNNPENGTYDVTLKINKTDISGVLQTFQRYNYFIKSAFQVDTSYFDDLKSRYDALMN
                     YLKL"
     gene            1092..>1397
                     /locus_tag="PK610_13960"
     CDS             1092..>1397
                     /locus_tag="PK610_13960"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRJ37096.1"
                     /translation="MTKLSVNINKIATLRNSRGGNVPDVIKVALDAERFGADGITVHP
                     RPDERHIRYDDVRKLKPLLSTEFNIEGNPKEKKFVDLVLEVLPHQVTLVPDGGSQLTS
                     "
BASE COUNT          426 a          281 c          277 g          413 t
ORIGIN      
        1 atcggccaga ggtaaaaacg ttcttttaaa aaaatgaaaa catctgaata caccgaatcg
       61 atcgggactt cctttacctc tacaaaatta aaaagaagtg tattgatgag cattgattgt
      121 gagcgctcta tttctctgga gagttggttc cggtgaaatc tctgccctgg cataaaagaa
      181 atttcacgga gaataatact ctcttttgtt ttattattcc cactaataga aattctggca
      241 acggtaacag agtcgtgttg tgaatagaca gtaatcgccc gaatgaaaag tatagtaagg
      301 ataagtatcc tttttccggg cattctgatt ttaattacag tttaaggtag ttcatcaacg
      361 catcgtatct cgacttcaaa tcatcaaaat acgatgtgtc aacctgaaaa gcagacttga
      421 taaaatagtt ataacgctga aacgtttgaa gtacacccga aatatctgtt ttattgatct
      481 ttaaagtaac atcataagtg ccgttttcag gattgttatt caggaacaca cttaaaatct
      541 ttgcatcgtt tccctccaca atttgagcga tttgagataa ggaataatcc tgattactca
      601 cttcaagtac aagaacactt ccgggctcgg ttatggatga gaaggaccct attttatcca
      661 tgaggttggt catggaaatt gcccccatat agttaccatt gacatcaaga accggaataa
      721 tactcaaccg gttttcattc atgagtttta tggcatcata gatgtgttgg ttctcgctga
      781 tgaaaatatc atcaagctcc agatcttcct gttcacccaa ggctttatca ggctggttaa
      841 ggtcaagaat atcagcgtct gaaatcaatc ccaggtactt acctccgtca accacaggca
      901 gatgggcaac cttgaattca tccatccact tcagtgcttt gttgcctgta tcgctcattt
      961 taatgggcgg aatgttctct gatatgaggt cttgagtcag cataattttc aggcttcaaa
     1021 tatcgtgcag aaatttcatt gtaattttgg tttctgcaag gtgaggcaaa aatacgaaat
     1081 acattacacc tatgacaaag cttagcgtca atatcaataa gatagcaaca ttacgcaact
     1141 cccgtggagg taatgttccg gatgtaatta aagttgccct ggatgctgaa cgcttcggcg
     1201 cagatggaat aacggttcac ccacgtcctg acgaaagaca tattcggtac gacgatgtac
     1261 ggaaattgaa acccttattg tctaccgaat tcaatatcga aggaaatccg aaagagaaga
     1321 agtttgttga tctcgtattg gaagtgcttc ctcatcaagt tacactagtc ccggatggcg
     1381 gatcgcaatt gacctcc
//
LOCUS       DAOMYG010000585         1395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_38026_length_1395_cov_6.860448, whole genome shotgun sequence.
ACCESSION   DAOMYG010000585 DAOMYG010000000
VERSION     DAOMYG010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38026_length_1395_cov_6.860448"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="PK610_13965"
     CDS             complement(<1..1036)
                     /locus_tag="PK610_13965"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRJ37097.1"
                     /translation="MSEKAPKQKGSWKKARRFLKYVGPYRVAWYASFVFLLLSSALSM
                     AFPLLMGRLFDAGTSTSGIDLSDMSSANSVAILLFIVFGVQSVFSYFRIYLGSIVTEG
                     ALNDLRRDAYKKLVSLPISFFNKNKVGELNSRISADITMLQDTFNTTLAEFVRQFIVI
                     IIGISALAWISWKLSLIMIATIPVMAIVAVFFGRFIKRLSKEAQDKVAESNGIVEETL
                     TAIASVKAYANEAFEVVRYKKAADEIKNLSMRGAVWRGAFVSFIIFAIFGSIVFVLWQ
                     GVLLTQNGEIAKGDLVSFVVMSVFLGASFGSIPELFARIQKSIGASERLMDLFDETSE
                     NISMEIEKKGM"
     gene            1207..>1395
                     /locus_tag="PK610_13970"
     CDS             1207..>1395
                     /locus_tag="PK610_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ37098.1"
                     /translation="MERYDIVVIGGGPAGYAAAMRAIDFGKKVLLIEKDKVGGYGIYD
                     GALSSKTMWELSEKVSSVN"
BASE COUNT          429 a          314 c          266 g          386 t
ORIGIN      
        1 ccattccctt tttttcaatt tccattgaaa tattttcaga agtctcatca aaaagatcca
       61 tcaaacgttc gcttgcacca atggattttt gaattcgtgc aaacaattcg ggaatactac
      121 caaatgatgc accaaggaaa accgacatca ccacaaatga aaccagatcc cccttagcta
      181 tctctccatt ttgtgttagc agcacacctt gccaaaggac aaatacaata gaaccaaaaa
      241 ttgcaaaaat gataaacgaa acaaatgcgc ctctccacac tgcaccccgc attgaaagat
      301 tttttatttc atctgcagct tttttatagc gtaccacttc aaatgcttcg ttggcatagg
      361 cttttacgct ggcgattgca gtcagtgttt cttcaacaat tccatttgat tcggccactt
      421 tatcctgtgc ttctttagag agtcgcttga tgaatcgtcc gaaaaacact gcaacaattg
      481 ccatcaccgg aatagttgca atcatgatca gagaaagctt ccagctgatc catgcgagag
      541 cggaaattcc tataatgatt acgatgaact gacgaacaaa ctccgccaaa gtagtattga
      601 atgtatcctg cagcatggtg atatctgcac tgatgcggct gtttagttct ccgactttgt
      661 ttttattgaa aaatgaaatc ggtaacgaca ccagtttctt gtacgcatcc ctgcgaagat
      721 cattcagtgc tccctcggta acaatactcc ccaaataaat tctgaaatac gagaataccg
      781 attgaacccc aaaaacaata aacagcaaaa tggccacgct gttggcactg ctcatatccg
      841 ataaatcgat tccggaagta ctggtaccgg catcgaaaag tcgccccata agcaatggaa
      901 aagccatgga taatgcactg gataacaaca agaaaacgaa actggcgtac caggcaacac
      961 ggtaaggacc aacatatttc agaaatctgc gggccttttt ccaggaaccc ttttgttttg
     1021 gtgctttctc gctcatcggg tggcaaattt aatgaataag acgcttgtgc aggggtatta
     1081 tgctaattga gttgaaatac cctgatttta attatcagat caagctcaca ttaacaaatt
     1141 tcgactcgat ctacaatcaa aaatcaacct ttaattgcta catttcggcc tgttttaaat
     1201 cctgttatgg agagatatga cattgttgtg attggaggtg gtcctgccgg atacgcagcg
     1261 gccatgcgtg ctatcgattt cgggaagaaa gtacttctga ttgaaaaaga taaagttggt
     1321 ggatacggaa tctacgatgg agccctttct tccaaaacca tgtgggaact ttccgaaaag
     1381 gtttcttcgg ttaac
//
LOCUS       DAOMYG010000586         1394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_38062_length_1394_cov_8.934279, whole genome shotgun sequence.
ACCESSION   DAOMYG010000586 DAOMYG010000000
VERSION     DAOMYG010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38062_length_1394_cov_8.934279"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="PK610_13975"
     CDS             <1..1056
                     /locus_tag="PK610_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37099.1"
                     /translation="TDFILKIFTYKRKVESAEKLYEEALITLNKKASDSNLAYELIGK
                     NKYGVLNYDPIAKDFLTQEEHPIDFNKKTLLLLHGTFVNVWKTYDPLFNDKFGNPPLL
                     NRLLADGKFEQIIALNYATISHDAEQNAKVFYELLGEGNRFTQAVSIVGSSRGCLLAK
                     FLSADPNNEAFTADKVIMFSGANGVGFFTTGKHIARFLSIMRRISGPGGKVALALLQF
                     SAEYFLSLPGAQQMTPKSATLKRVLEMNPHPDTEYFNVVSDWNTSLTERWQQKIFGGG
                     LDLLLKGILGREHDWVVGCPEQELYFTENGNTLSPRRIVSMHARYFDIHYTKDHNTHE
                     IIWEYFPDMNSSDRPLV"
BASE COUNT          434 a          287 c          273 g          400 t
ORIGIN      
        1 accgatttca tcctgaaaat attcacttac aaacgcaaag tagaaagcgc tgaaaaactg
       61 tatgaggaag ccttaattac gttaaacaaa aaagcttccg atagtaatct tgcctatgaa
      121 ctcatcggaa agaataaata cggagtactc aattacgatc ccatcgcaaa agattttctt
      181 acacaggaag aacatccaat cgatttcaac aaaaaaacac ttctgttgct gcatggaact
      241 tttgttaacg tctggaaaac ctacgatccc ttgttcaatg ataagttcgg caatccgcct
      301 ttactaaatc gcctgcttgc agacgggaaa tttgaacaga tcatcgccct gaattatgca
      361 accatctctc atgatgcgga acaaaatgca aaagtgtttt acgaacttct cggcgaagga
      421 aatcgtttta cacaagccgt ttcaatcgtc ggatcaagtc gcggttgtct gctggcgaaa
      481 tttctgagcg ctgatcctaa caatgaagcg ttcactgccg acaaagtaat tatgttttcc
      541 ggagccaacg gagtgggatt ttttaccacc ggaaaacata ttgcccgttt cctctccatc
      601 atgcgcagaa tttcaggtcc tggaggaaag gttgcgctcg cattgttgca attttcggcg
      661 gagtattttt taagtcttcc gggtgcacaa caaatgacgc ccaaaagcgc aaccttaaaa
      721 cgtgtacttg aaatgaatcc gcatcctgat actgaatatt tcaatgtagt atccgattgg
      781 aatacctcct taacggagcg atggcagcaa aaaatatttg gtggcggact cgatctcctt
      841 cttaaaggca ttcttggtcg tgagcacgat tgggttgtag gttgccctga gcaggaactt
      901 tattttacgg aaaacggaaa tacactttca ccccgacgca tcgtatccat gcatgcgcgt
      961 tatttcgaca tccactacac caaagaccac aatacacatg aaataatttg ggagtacttt
     1021 ccggatatga acagtagtga tcggccttta gtttagtaag gcttcgattg tttattcgga
     1081 taatccggac aatgaatctt tgcgttgtca aaaattgtaa ttgctgatat agtaaatatg
     1141 agctgaattc ttagttcact gaattcttgg ttcactgaat tctgcaccaa aaaagtgttg
     1201 tgactagtta caaaattgct taagatttac tgcatcctaa agaatagctc gaaatagaga
     1261 taactgaacc atctgaaagg aaaaataaac aggtttggta aacatataga ggacttgtat
     1321 gttatgctta ttggaattta ctttccaaat ggccagttca ttgattacac attccatttt
     1381 cacaatattt aaca
//
LOCUS       DAOMYG010000587         1378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_38744_length_1378_cov_9.076342, whole genome shotgun sequence.
ACCESSION   DAOMYG010000587 DAOMYG010000000
VERSION     DAOMYG010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38744_length_1378_cov_9.076342"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..>1378)
                     /locus_tag="PK610_13980"
     CDS             complement(236..>1378)
                     /locus_tag="PK610_13980"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRJ37100.1"
                     /translation="SSYAQLPVFTLDQARARINENHLLMKNSELQIEYEKEQKGQNRP
                     LPPLELVMQYGQINYDRRDWYMESNQSLGNFASWFRMDQVMRRKIILAESQQALLQRE
                     LLLQVSIAYTGWKYAWQERDMYDSLLVIHDRALKRMEKKMNSGGSDRSEYLLLSSRQA
                     SLRNEFLFAEMRLWNLWNELATLCMYSDSAVQPESVKSATFMVKPTTVLPELLSTSEN
                     AIEIQRAETALIRSGYSPELMIGYFRQSLENIGGYWGVRAGISIPLWAWTVRSKAEQS
                     KIREQQLTNDLEWQRFQLAQQLNSLVRQSVVLSDALQRNGSDYLNDLEKLRMLAEKQY
                     QSGAISFFDFRQMIDHYLRSRIDHVRLLNEYENLIHQIRFLTDNTY"
BASE COUNT          388 a          303 c          254 g          433 t
ORIGIN      
        1 agtatgaata tcagcaaggt tttgcggagg aacttcaatt tttccgttgc acaaaacttc
       61 actgctcatt tcacgaaact cgggttttcc ccaggagatt ccattggctg aaatttgttc
      121 ctcagttaac tttatttcct caggagctgc agtttccatt tctgacaatt gaggagtact
      181 gcaggctatt aaaaaaatag cgaagctgaa tgtatatata atttgatttt tcattttagt
      241 aggtattatc agttaagaaa cgaatttgat gaatcagatt ttcatattca tttaacaaac
      301 gaacatgatc gatgcgggat cttaaataat gatcgatcat ttgtctgaaa tcaaaaaagg
      361 aaatagcacc ggattgatat tgcttttctg caagcattcg taatttttca agatcattca
      421 ggtaatcgct tccgtttcgc tgtaacgcgt cggataaaac aacagattgt cgcaccaatg
      481 aatttagctg ttgtgccaat tgaaatcgct gccactcaag atcgttggtt aactgttgtt
      541 ctcttatttt tgattgttct gcttttgaac gaactgtcca ggcccaaagt ggaatactta
      601 tcccggcacg gactccccaa taacctccta tgttttccag cgactggcga aaataaccta
      661 tcatcaattc aggtgaataa ccactgcgaa tcaatgctgt ttctgcgcgc tgaatttcaa
      721 ttgcattttc acttgttgaa agcaattcag gcaatacagt agttggttta accataaatg
      781 ttgctgattt taccgactcc ggctgaacag cagaatcgct gtacatacaa agtgttgcca
      841 gttcattcca caaattccag agacgcattt ctgcaaataa aaactcgttt cggagagaag
      901 cctggcgcga actcagcagc agatattctg aacgatctga tccgccacta ttcatctttt
      961 tctccattcg cttaagcgca cgatcgtgta ttacaagtag cgaatcatac atatcacgct
     1021 cctgccacgc atatttccat cctgtgtacg caatagaaac ctgcaataat aattcgcgtt
     1081 gcaacaatgc ctgctgcgat tctgcaagga tgatttttcg ccgcattacc tgatccatcc
     1141 gaaaccagga tgcaaaattc ccaagcgatt gattggattc catgtaccaa tcccgtcggt
     1201 cgtaatttat ttgtccatat tgcatcacta actccaaagg tggtaacgga cgattctgac
     1261 ccttttgttc cttttcatat tcaatctgca attcactgtt cttcatcaat agatgattct
     1321 cattgatcct tgctctggct tggtccagtg taaataccgg taattgtgca tacgaaga
//
LOCUS       DAOMYG010000588         1364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_39386_length_1364_cov_11.804431, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000588 DAOMYG010000000
VERSION     DAOMYG010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39386_length_1364_cov_11.804431"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PK610_13985"
     CDS             complement(<1..443)
                     /locus_tag="PK610_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37101.1"
                     /translation="MKLRISHPHTLLLAITFGSASLAVAGPGNTDGNNDKSTETSTDT
                     KTTPVPFNLNSGEWVIIEVIEDNKPQPQPDKVTVERKNISQMTVTKVEVRSCQKNTTP
                     EQLIPILREINQTVNHRRVYMQEENGKKIWYISPEVKETPQVNTPA"
     gene            complement(512..1264)
                     /locus_tag="PK610_13990"
     CDS             complement(512..1264)
                     /locus_tag="PK610_13990"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphatase"
                     /protein_id="HRJ37102.1"
                     /translation="MGFLGGLFGRRKKEILPPADLSLLGTDVHSHFIPGIDDGAKTME
                     DSLSMLRTMQEFGYKKVITTPHIMSDFYRNTPEIILSGLDKLREAAHKENIDITIEAA
                     AEYYLDIELEDKIRKKELLTFGNNYVLFEMPFVGEPKNLSRAIFEMQMAGYKPVLAHV
                     ERYLFWHREPEKAQTLFDKGVILQLNLNSLSGHYSPQVQKSAEQLLDSEIIGLLGTDC
                     HNMGHLELLKQSSTLPYFHKAMKINNLLNHSL"
BASE COUNT          350 a          265 c          294 g          455 t
ORIGIN      
        1 gcaggcgtat tcacttgagg agtttcttta acttcaggtg agatgtacca gatttttttt
       61 ccgttttctt cctgcatata aacccgacga tggtttactg tttggttaat ctcgcgtaag
      121 attggaatga gttgctccgg tgtggtgttt ttctgacaac tgcgaacttc aactttggtg
      181 actgtcattt gcgaaatgtt ctttcgttca acagtaactt tatccggctg aggttggggt
      241 ttattgtctt caatcacttc aattatcacc cattcgccac tgttcagatt gaaagggacg
      301 ggtgtagttt tggtgtcggt agaagtttcg gttgatttat cattattccc atccgtattt
      361 cccggacctg caacagctaa gctggccgaa ccgaaagtga ttgccaggag taaagtgtgt
      421 ggatgtgaaa ttcgtagctt catacgtatg actcaaatat acgtattcag cgactaataa
      481 gttgagtgtt aacaattttt tgaatctcca tttacaagga atgattcagc aaattattga
      541 ttttcattgc tttgtgaaaa tagggtagtg ttgaactttg tttcaacaac tccaggtgac
      601 ccatgttatg acaatccgtt ccaaggagtc caatgatctc tgaatcaagt aattgttctg
      661 cagatttttg cacttgtggg gaatagtgcc cactcaggga gttcaggttc aattgaagga
      721 taacaccctt atcgaacagg gtttgtgctt tttcaggttc gcggtgccag aataagtagc
      781 gttcaacatg tgccaagacg ggtttgtagc ctgccatctg catctcgaaa attgccctac
      841 ttagattttt aggctcgcca acaaaaggca tttcaaaaag gacataatta ttaccaaatg
      901 tgagcaactc tttttttcgg attttatctt ccagttcaat atcgagatag tactcagctg
      961 ctgcttcaat agtaatgtcg atgttttctt tgtgtgcagc ttctctgagt ttatcaagtc
     1021 cgcttagaat tatttccggt gtattgcggt aaaaatccga catgatatgc ggagtggtaa
     1081 tgaccttttt atagccaaac tcctgcattg ttcggagcat gcttagggag tcttccatgg
     1141 tttttgcacc atcgtcaatt cccggaataa aatgcgaatg cacatcagtt cccagcaaag
     1201 aaagatcagc aggtggtaaa atttcttttt ttcttctccc gaataatccg cccaaaaaac
     1261 ccatgatcaa ataaaatctt agcgatgtgt atattgttgt tcgtagtaat tacggtattc
     1321 tccggatgtt actctgtcaa gcgatgcagt gtttcggagg tacc
//
LOCUS       DAOMYG010000589         1340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_40450_length_1340_cov_8.431907, whole genome shotgun sequence.
ACCESSION   DAOMYG010000589 DAOMYG010000000
VERSION     DAOMYG010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40450_length_1340_cov_8.431907"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..>1340)
                     /locus_tag="PK610_13995"
     CDS             complement(42..>1340)
                     /locus_tag="PK610_13995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HRJ37103.1"
                     /translation="VKNLTWTFAYNQSNYPASITIPCMMIGGWYDHASDAMLSYFSAL
                     RTTSPLAVRNKHRLIMGPWVHGGHGAAYVGSALQGELSYPGAEGWGDSLALAFLDHYL
                     RGISNGIDLTEYVTYFQMGEDEWNVAPSWPPSGVSPIKLYLQNDFSLGTVLPSNGTGA
                     SLITFDPRNPSPTIGGPTLRSDLDQGPYNQAPLVESRNDVLIFESPVLGAPVVMKGNV
                     RAHFYVSSNRKDTDVAVRITDVYPDGRSMILLDGIRRMRFRNGFTAADTASMIPGQIY
                     EVDVQLMNSAITFLPGHRIRLDITSSNYPRFDVNLNNGGAMYVAGDTLVAQNTIYHNA
                     SQASYIELRLEDFIGETPEDLFVGHKLFPNPANEFLELTVPGNSEFSWEIFDVQGKSQ
                     KSGTCKGENRVVVAELYPGLYVLQYTINGKSYRVKWVKSK"
BASE COUNT          416 a          297 c          259 g          368 t
ORIGIN      
        1 aatggaaagg aacaacaaaa aaaataagat ccaataattt ttcattttga ttttacccac
       61 tttacacggt aactttttcc attaattgtg tattgcagga catacaatcc gggataaagc
      121 tcagcaacca ctaccctgtt ttctccttta cacgttccgc ttttctgtga ttttccctgc
      181 acatcaaata tctcccagga aaattcggaa tttccaggaa cagtcaactc aagaaattca
      241 tttgctggat ttggaaacaa tttatgtccg acaaataaat cctctggtgt ttctccaata
      301 aaatcttcaa ggcgaagctc aatataagag gcctgactag cgttgtgata aattgtgttt
      361 tgcgcaacaa gcgtgtcacc cgcaacatac attgctccac cattattcag attgacatca
      421 aaccgcggat aattggaaga agtaatatct aaacgaattc tgtgtcccgg caaaaaggta
      481 attgcgctgt tcatcaattg aacatccact tcataaatct gacccggaat cattgaggct
      541 gtatcagcgg ctgtaaatcc atttctaaat cgcattcttc tgatgccatc gagtaaaatc
      601 attgatctgc cgtccggata aacatcggta atacgaacag caacatcagt atcttttctg
      661 tttgaagaaa cataaaaatg tgcgcggaca tttcctttca taactacagg tgctcccaat
      721 acaggagact caaatatcaa tacatcattt cttgattcca caagcggagc ctggttgtaa
      781 ggtccttgat cgagatcaga tctgagtgtt ggtccaccaa tagtgggtga aggattacgc
      841 ggatcaaagg tgattagtga cgccccggtc ccattggaag gaagaactgt tccgagagaa
      901 aaatcgtttt gcaggtataa ttttatagga gaaacacctg aaggaggcca tgatggagca
      961 acattccatt catcctctcc catttgaaaa taagtgacgt attccgttaa atcgattccg
     1021 ttcgaaattc ctctcaaata atgatcgaga aaagccagcg caagagaatc gccccaacct
     1081 tctgctccgg gataactcaa ttctccctgt aacgctgaac caacatacgc agcaccatgt
     1141 ccgccatgaa cccatggccc cataattaac cgatgcttat ttctaacggc aagtggagag
     1201 gttgttcgca atgcagaaaa ataggaaagc attgcatcag aagcatggtc ataccaccca
     1261 ccaatcatca tgcagggaat agttattgaa gctggatagt tggattgatt gtatgcgaat
     1321 gtccatgtta ggtttttgac
//
LOCUS       DAOMYG010000590         1335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_40689_length_1335_cov_5.694531, whole genome shotgun sequence.
ACCESSION   DAOMYG010000590 DAOMYG010000000
VERSION     DAOMYG010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40689_length_1335_cov_5.694531"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /gene="mutY"
                     /locus_tag="PK610_14000"
     CDS             complement(<1..896)
                     /gene="mutY"
                     /locus_tag="PK610_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRJ37104.1"
                     /translation="MTDFASTIYRWYTQNARKLPWRETREPYLIWISEVILQQTRVDQ
                     GMAYYHHFAKNYPTVEKLASATEEQILKSWQGLGYYSRARNLHATAQIIVKEYKGKFP
                     ATYSGLLQLKGIGPYTAAAISSFAYREKKAVVDGNVSRVISRYFGIEEPIDSTTGKKL
                     IQAIADEHIPEKRPDLFNQSIMEFGALQCTPKNPNCRDCPLQTGCFAFRHKKVDLLPI
                     KDKKTKVSSLFLHYFFVSDSTKYYIRKRGSDSIWKNMYELPCTANEKESKTGKIHIDK
                     NWKIPASATFRFDVAAYRHQLS"
     gene            1034..1324
                     /locus_tag="PK610_14005"
     CDS             1034..1324
                     /locus_tag="PK610_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRJ37105.1"
                     /translation="MTKADIVAEIAEKTGVEKVAVQVTVEAFMNSIRESLERGNNVYL
                     RGFGSFIVKKRAEKTGRNISKNQAIIIPAHYIPSFKPAKTFVDRVKSKVKVK"
BASE COUNT          366 a          265 c          293 g          411 t
ORIGIN      
        1 tggcttaact gatgcctgta tgccgccacg tcaaaacgga atgttgcaga ggccggaatc
       61 ttccagtttt tgtcgatatg gattttaccg gttttacttt ctttttcatt tgctgtacaa
      121 ggtaattcgt acatattttt ccaaatggaa tctgacccgc gttttctgat gtaatacttc
      181 gtgctgtcgg atacaaaaaa atagtgcaga aaaagagaac ttacctttgt tttcttatcc
      241 ttgatcggta gcaaatcaac ttttttatgg cggaatgcaa aacaacccgt ttgtaagggg
      301 caatctctgc aattcggatt ttttggagtg cattgcaagg cgccgaactc cattatcgat
      361 tgattgaaca aatcaggacg cttttcagga atatgttcat ctgcaatggc ctgaatgagt
      421 tttttgcctg ttgtagaatc gatcggttcc tcaataccaa aataccggga aattaccctt
      481 gatacatttc cgtcgacaac cgccttcttc tctctatagg caaaagagga aatggctgct
      541 gctgtgtaag gaccaattcc tttaagctga aggagtcctg aataagttgc aggaaatttt
      601 cctttgtatt cctttacaat gatttgggct gtagcgtgca ggttgcgagc tctgctgtaa
      661 taacccaatc cttgccagct tttcagaatt tgctcttccg ttgcggaggc taatttttct
      721 actgtggggt agttttttgc aaaatgatgg tagtaggcca tgccttgatc gaccctcgtt
      781 tgttgcaaaa tgacctccga aatccaaatt aaatagggtt cgcgggtctc gcgccagggt
      841 agttttcggg cgttttgggt gtaccaacgg taaattgttg aggcaaaatc agtcattcgg
      901 acgtaaaatt ggtatttccg tctaaaaaac aactacttcc atcgcaatgt tcttgcaagt
      961 tattgaggtt taagggtata tttgtgtgga acactaaaga aaaagaaacc agttaatctt
     1021 aaaaacacta cttatgacca aggctgacat cgttgctgaa atcgctgaaa agaccggagt
     1081 tgaaaaggtc gcagtacagg ttactgttga agcgtttatg aattctatcc gcgaatcgct
     1141 tgagcgtggt aacaatgttt acctccgtgg gttcggaagt ttcattgtga aaaaacgcgc
     1201 tgagaaaacc ggaagaaaca tctccaaaaa tcaggcgatc atcatccctg cgcattacat
     1261 tccttcgttt aaacctgcca agactttcgt tgatcgcgtt aagtcgaagg taaaagtgaa
     1321 ataatgaaat cttct
//
LOCUS       DAOMYG010000591         1329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_40945_length_1329_cov_10.763736, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000591 DAOMYG010000000
VERSION     DAOMYG010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40945_length_1329_cov_10.763736"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..>1329)
                     /locus_tag="PK610_14010"
     CDS             complement(193..>1329)
                     /locus_tag="PK610_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37106.1"
                     /translation="GMVIEGTQLKLFSLPDGTILRNWVLPGFSSYPEPRYAEQFLRGI
                     LSPDGTQIVVYFKGSFYNAATWTDEPEFKLYTPSSVNIQAGMEFYCSNKAKHVVTTEI
                     PGSGDQSGSGQCSPGYRVPVMVMRNSAVKSFTERIACVFTSHVAVDEEGNVAAYGDKG
                     MLYYPVNGSSPVQLAGKDVRLIKQSFCMNGNALLVNARVPLNDSLSKLKEERIARELD
                     RQMKNYRDIQPDDISAEVAYLSDRSKKEEWLRFCYPDSVWSLVWIKKTGDGWSKPEPA
                     LPPHLYPIAWSTAVSPDGKTIAWVELERGDDGNMIVSKKLQFVQRLENGKWRGPVVVT
                     DMPDHNNHIWLFDQYGVVYHEGKLQLFYFSGKQSGKLKVINMKE"
BASE COUNT          369 a          329 c          245 g          386 t
ORIGIN      
        1 tgacggcaaa ttggtaattg aaaaatttgc aacaggaaac tcaacacaac tcttcgtaca
       61 atcacttgaa agaggaatct acctgataaa actcactgca actgatgctg tttattttgt
      121 gccgttaatt ctcaccaatt aataaaaaaa gtcccgcaaa tgcgggactt tttttgaatg
      181 tgctgctatt atttattctt tcatgttgat caccttcaac ttacccgact gtttgccact
      241 aaagtaaaat agttgcaatt ttccttcgtg ataaacaact ccgtattgat caaacaacca
      301 gatgtgattg ttgtggtcag gcatgtcggt taccacaaca ggacctctcc attttccatt
      361 ctccaatcgc tgtacgaatt gtaatttttt gctcacaatc atattaccgt catcacctct
      421 ttccaattct acccatgcaa ttgtttttcc atccggagat actgcggtcg accaagcaat
      481 gggatacaaa tgcggaggca atgcaggttc aggcttcgac caaccatcac cggtcttctt
      541 gatccatacc agcgaccata ccgaatcggg ataacaaaac ctcagccatt cttctttttt
      601 gctcctatcg ctcaggtaag ccacttcagc agagatgtcg tcaggctgaa tatctctgta
      661 gtttttcatc tgacgatcca gttcgcgcgc gatgcgttct tctttcaatt tactaagact
      721 atcattgagg ggcactctgg catttacgag caaagcgttt ccgttcatgc aaaacgattg
      781 cttaataaga cgaacgtcct ttcctgcaag ctgaacagga gaacttccat tcacaggata
      841 atacaacatt cccttatctc cataggcagc aacattccct tcttcatcca cagcaacatg
      901 gctggtaaaa acacaggcta tccgttcagt gaaacttttt acagcggaat tacgcatcac
      961 catcaccgga actctgtacc cgggagaaca ttgtccggat cccgattgat ctcccgaacc
     1021 cggaatttcg gtggtgacaa catgttttgc tttattcgag caatagaatt ccattcctgc
     1081 ctgaatgttg accgagctcg gtgtatacaa cttaaattcc ggctcgtcgg tccaggttgc
     1141 tgcgttataa aaactgccct taaaataaac aacgatttgt gttccgtcag gacttaggat
     1201 tcctcttaga aattgttcgg cataacgtgg ttccggataa cttgaaaacc caggtaaaac
     1261 ccaattgcga agaattgtgc cgtcgggaag tgaaaacaac ttcaattgcg ttccttcgat
     1321 caccattcc
//
LOCUS       DAOMYG010000592         1311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_41829_length_1311_cov_6.734873, whole genome shotgun sequence.
ACCESSION   DAOMYG010000592 DAOMYG010000000
VERSION     DAOMYG010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41829_length_1311_cov_6.734873"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PK610_14015"
     CDS             <1..911
                     /locus_tag="PK610_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HRJ37107.1"
                     /translation="EELIHSSGKKNPKKFPLVLSTWNFGLKANEEAWKILSAGGRAID
                     AVEQGVRLVEADPDERSVGYGGRPDRDGRVTLDACIMDENMNIGSVACLEHIKHPISV
                     ARAVMEKTPHVMLVGDGALQFALEKGFQKENLLTEASEKEWKEWLKTAKYQPVVNIEN
                     HDTIGMVAMDTSGNLSGACTTSGMAFKMHGRVGDSPIIGAGLYVDNEVGAVTATGHGE
                     EVIRIAGCHLVVELMRQGMSPEKACKTAVERVCRKQKHRINEVQVGFIAISSKGEYGA
                     YCIHPGFNYAVRTNERNSLIESKSIL"
     gene            complement(918..>1311)
                     /locus_tag="PK610_14020"
     CDS             complement(918..>1311)
                     /locus_tag="PK610_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37108.1"
                     /translation="PEKNNLLKEYSKNFFSTPLSMDEVFLRMELYSGNRMLFRDLFFF
                     VAENKFAWKPANISVELKKVSNDVTTARVSARTLVRKFFHGQHFNSTPNLIDILPNDT
                     LSFSITLPDVEAWKSGIVCLNDFMFQKN"
BASE COUNT          392 a          225 c          321 g          373 t
ORIGIN      
        1 tggaggaatt gattcattca tcaggaaaaa agaatccgaa aaaatttcct ttggttcttt
       61 ccacctggaa ttttggattg aaagcgaatg aagaagcctg gaaaattttg tcggcaggcg
      121 gacgggcaat tgatgcggtt gagcagggtg tacgattggt agaagctgat cctgatgaac
      181 gctccgttgg ttatggcggg cgacccgacc gtgatggccg tgttacattg gatgcctgta
      241 ttatggatga gaacatgaac attggttctg tcgcttgttt ggaacacatc aaacatccaa
      301 tttcagttgc acgtgcggta atggaaaaaa ccccacatgt aatgctggtt ggtgatggtg
      361 cattgcagtt tgcattggaa aaaggttttc agaaagaaaa tttacttacc gaagcttccg
      421 aaaaggaatg gaaggaatgg ctcaaaaccg caaaatatca gccagtagtt aatatcgaga
      481 atcatgatac cattggcatg gttgcaatgg atacaagcgg aaatctttca ggcgcctgca
      541 caacttcggg aatggcgttt aaaatgcatg gcagagttgg cgattctccg attattggtg
      601 caggtttgta tgtcgacaat gaagtaggtg ctgttaccgc tacagggcat ggggaagaag
      661 taattcgtat tgcaggttgc catttggtgg ttgaactcat gcgacaggga atgtcgccgg
      721 aaaaagcttg caagactgct gttgaacgcg tttgccggaa gcaaaaacac cgcatcaatg
      781 aagtgcaggt gggtttcatt gccatcagtt caaagggaga atatggggcc tattgcatcc
      841 atcccggatt taattatgct gttcgtacaa atgaaagaaa cagcttgatc gaatcaaaaa
      901 gcattctata ataaagttca atttttttga aacataaaat catttaaaca gacaattcct
      961 gatttccagg cttccacatc gggtaaggtt atggagaaac taagggtgtc gttgggaagg
     1021 atatcgataa gatttggtgt tgaattaaag tgttggccat gaaagaattt tctaaccaga
     1081 gttctggcgc tgactcttgc tgttgtaaca tcattgctca ctttctttag ttcaactgaa
     1141 atatttgccg gtttccaggc gaatttattt tctgctacaa aaaagaacaa atcgcgaaat
     1201 agcattctat ttccagaata taattccata cgcagaaaaa cttcatccat tgaaagtggt
     1261 gtggagaaaa agtttttaga atattctttt agcagattat ttttctcagg g
//
LOCUS       DAOMYG010000593         1300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_42351_length_1300_cov_7.442570, whole genome shotgun sequence.
ACCESSION   DAOMYG010000593 DAOMYG010000000
VERSION     DAOMYG010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42351_length_1300_cov_7.442570"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1249
                     /locus_tag="PK610_14025"
     CDS             158..1249
                     /locus_tag="PK610_14025"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HRJ37109.1"
                     /translation="MLLLAVNGQGQSAWHGGIQAHYGFLIAHRPSMVHLVENHVKSIE
                     PYIERSLSSKPWHMRYNEPMHGLSLNIGNLANPKMIGMGFGLIHYVKFKLAGNDNFSL
                     GLRLGTGVGYLTKTFDRTTNNKNNAIGSHFNGLMSTQLEFTQVWERFQLGAGLSLLHY
                     SNGAMTMPNLGLNMPSAFLSLKYRIKEPGAATVLHNSEYYGNQPRWTFSTLFSGGLRE
                     LGQPLGPKYTCFTLETFASRILNPKTNALFGIDVLNNGSLSKAWQTAMEEDVNRWKFT
                     QLGLMAGIELSMDELKISLQQGFYVISKYKGDGNYYQRLTLRYFFKNNMIAQISLKTH
                     FAKADYFELGLGYRFAKSKKGNGGSNGFK"
BASE COUNT          407 a          263 c          271 g          359 t
ORIGIN      
        1 aactcatcca taatggtaag taaacgcgca aatgcttcct ttttttgttc aatggcagtc
       61 atggcttaaa attacgaaaa ggaattcctt acaggtggtt tcaatttgta tttttatggg
      121 gatgtttcgc acaagatcag gaataataat gctttttatg ctgctccttg cagtgaacgg
      181 gcaaggacag tcggcctggc acggtggaat tcaagcccat tatggttttt tgatcgccca
      241 ccgtccttct atggttcatc tggtcgaaaa tcatgtaaaa tcaatagaac cctatatcga
      301 acgcagcctg agttccaaac cctggcatat gcgttacaat gaaccgatgc acggcttatc
      361 actcaacatc ggcaatctgg caaatccgaa aatgatcgga atgggatttg ggttgattca
      421 ttatgtaaaa ttcaaactgg caggtaacga caacttttca ctgggacttc gcctcggaac
      481 cggtgtgggg tatcttacca aaacatttga ccgcaccacc aacaacaaaa acaatgcgat
      541 tggaagtcat ttcaacggac tgatgtcgac acaactggaa tttacccaag tttgggagcg
      601 ttttcaatta ggggctggat tgtcattgtt gcattattcc aacggagcaa tgaccatgcc
      661 caacctggga ttaaatatgc catcagcatt tctatcgctc aagtatagaa ttaaagaacc
      721 gggagccgca acagtgcttc acaatagtga atattatgga aatcaaccca gatggacctt
      781 ctctaccttg ttttccggtg gtttacgtga acttggacaa ccactcggcc cgaagtacac
      841 gtgttttacc ttggaaacat ttgcttcaag aatattgaat ccaaaaacga atgcattatt
      901 cggaattgat gtgttgaaca acggatcatt gagcaaagcc tggcaaaccg caatggagga
      961 agatgtaaac cgctggaaat tcactcaact cggcttaatg gcgggtatag aattgagcat
     1021 ggacgaatta aaaatctctc ttcagcaagg attttatgtg atcagcaaat acaaaggcga
     1081 tggaaattat taccaacgtc taacactacg gtactttttc aaaaacaata tgatagcaca
     1141 aatttcatta aaaacacatt ttgcaaaagc agattatttt gaattaggac tgggatatcg
     1201 ttttgccaaa tcgaaaaagg gaaatggagg aagtaatggt ttcaaataat tatttcccaa
     1261 agtccattca gcaattgcgc ctgatcattt cgatggcaat
//
LOCUS       DAOMYG010000594         1292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_42746_length_1292_cov_8.464026, whole genome shotgun sequence.
ACCESSION   DAOMYG010000594 DAOMYG010000000
VERSION     DAOMYG010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42746_length_1292_cov_8.464026"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="PK610_14030"
     CDS             complement(<1..1280)
                     /locus_tag="PK610_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37110.1"
                     /translation="MNNGIFSILPWTGNQLFFAIRERGLFLFDPDTKQFSPLKMEGYL
                     KDISFNDACLLNHPQYRVAISSSGHGILLLDHSGTFARKIHRASGLNTEEIRDIFTDR
                     SGNLWGVSPSGVHFMRINNPFEWYGSRQGIEGEVESVNKLKDDILVGTSSGLFINDEN
                     PRSLFRNTELVKGHVYEVHSHFDTRTGKELISICSNTGLYIMNGNEIKKISHDIFNTA
                     AYDPDKQILLAVGRSGIYAFAGPDWRLSHNESFSITRGLKIERDQLLSNSTVSVFFVG
                     LVEQGVIKILLSRDGTFTAVNYDRTDGLSSGMVRPFTYRNEVLFATDNGLQYFISEDE
                     IRAGLPDSLKGNPDYERGMFDMYTLAGKTWTQPISGIAAKQNNGLWMVVDNQIWSVND
                     KGEIDSTTFLDTDPGMINSIYSPDGIHLFISGSNG"
BASE COUNT          354 a          295 c          266 g          377 t
ORIGIN      
        1 ccgttcgatc cggaaataaa aagatgaatg ccgtcgggac tgtaaattga attgatcatt
       61 cccggatcgg tatccagaaa agtggtggaa tcaatttctc ctttatcgtt caccgaccag
      121 atctggttat ccactaccat ccataatccg ttgttctgtt ttgcagcaat ccctgaaatc
      181 ggttgggtcc aggtttttcc ggccagtgtg tacatatcaa acatgcctct ctcataatcc
      241 ggatttcctt tcagcgaatc gggaagtcct gcacggatct cgtcctcgct gataaaatat
      301 tgtaaaccat tatcggttgc aaatagtact tcgttacggt aggtaaatgg tcgaaccata
      361 cccgacgaca aaccatctgt tctgtcgtaa ttcaccgcgg taaatgttcc gtctctgcta
      421 agcagaatct tgatcactcc ttgctctacc agtccaacga aaaaaacaga aacagtggaa
      481 ttcgataata gctgatccct ttcgattttc aatcctctgg tgatgctgaa cgattcattg
      541 tgactcaatc gccagtcggg tcctgcaaag gcgtagatac cgcttcttcc aacagcaaga
      601 agaatttgtt tatccggatc gtaagctgcg gtattgaaaa tatcatgact gatttttttg
      661 atctcatttc cgttcatgat gtaaagaccg gtgttggaac atatactgat cagttctttt
      721 cctgttcgcg tatcaaaatg agaatgcact tcatatacat gtcctttcac caattcagta
      781 ttcctgaata aggaacgcgg gttttcatca ttgatgaaca atccggatga tgtacctacc
      841 agaatatcgt ctttgagttt gtttacactt tcaacttcac cttctattcc ttgtcgcgag
      901 ccgtaccatt cgaatggatt attaatacgc ataaaatgaa ccccggaagg agaaacgccc
      961 caaaggtttc cgctgcggtc ggtaaaaata tcacggatct cctcggtatt caatccggat
     1021 gcacggtgta tttttcgggc gaatgttccg ctgtgatcca gaagcagaat gccgtgaccc
     1081 gaacttgaaa tcgcgaccct gtattgcgga tgattcagaa gacaagcatc gttaaaactg
     1141 atgtctttta aatagccctc cattttaagc ggactgaatt gtttggtgtc aggatcgaag
     1201 aggaacaagc ctctttcgcg aattgcaaaa aacaattgat tgcctgtcca tggaagaatg
     1261 gaaaagatac cgttgttcaa caccagtgta tc
//
LOCUS       DAOMYG010000595         1271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_43846_length_1271_cov_8.796875, whole genome shotgun sequence.
ACCESSION   DAOMYG010000595 DAOMYG010000000
VERSION     DAOMYG010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43846_length_1271_cov_8.796875"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PK610_14035"
     CDS             complement(<1..380)
                     /locus_tag="PK610_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRJ37111.1"
                     /translation="MGNGIYYKTADEIELMRKSAQLVSKTLGAIAEIIGPGITPLQID
                     KIAETVIRDNGGTPSFLGYHDYPNTLCVSVNQTVVHGIPTSKAFHEGDIVSVDCGAIL
                     NGFHGDHAYTFAIGEIKPEAKKLL"
     gene            478..>1271
                     /gene="bshA"
                     /locus_tag="PK610_14040"
     CDS             478..>1271
                     /gene="bshA"
                     /locus_tag="PK610_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HRJ37112.1"
                     /translation="MKIGIVCYPTFGGSGVVATELGMALAASGHQVHFITYDLPVRLE
                     KTLENVFYHEVRVSDYPLFDYQPYELVLTSKLVDVVKYEKLDLLHVHYAIPHASAAYM
                     ARQILRSQGINIPYITTLHGTDITLLGKDESFEPVITFAINESDAVTAVSESLRQDTL
                     KFFRVKRDIKVIPNFICEEEYNVPVLENLKRSYAPNGEFILTHISNFRPVKRVDDVVR
                     VFELVRKKLPSKLLLVGDGPERSHVEKMCRESGLGTDVIFVGKMKQT"
BASE COUNT          350 a          253 c          296 g          372 t
ORIGIN      
        1 tgtaaaagtt tttttgcttc cggttttatt tccccaatcg cgaacgtgta ggcatgatcg
       61 ccgtgaaaac catttaaaat tgcaccacag tcaaccgaaa caatatctcc ttcatgaaat
      121 gctttgctgg tggggattcc gtgtacaacc gtttggttta cggaaacaca gagggtgttg
      181 ggataatcgt gataacccag aaaggaaggg gtaccgccat tgtcgcgaat taccgtttct
      241 gcaattttat caatctgcag cggtgttatt cccggaccaa ttatttcggc gattgcacca
      301 agtgtttttg aaaccaactg ggcgctctta cgcattagtt cgatctcgtc ggctgttttg
      361 taatagattc cgttacccat gggtgcaaaa atgccaataa ttcatgaatt gtggaaacga
      421 aacaatcggg gacgtatttc tgtttaaatt gcggatcaaa aaattactgg tggctcaatg
      481 aaaataggaa ttgtgtgtta ccccacgttc ggtggatctg gagttgtggc aacggaattg
      541 ggaatggctc tggctgccag tggtcaccag gtgcatttta ttacctacga tctacccgta
      601 aggctggaaa aaacccttga aaatgttttt taccacgaag tccgggtgag tgattatcct
      661 ttgttcgatt atcaacccta cgaactggtt cttacaagta agctggtgga tgtggtgaaa
      721 tacgagaaac ttgatctgct gcatgtacac tatgcaattc ctcatgcaag tgctgcttat
      781 atggcgcgac aaattcttcg ctcgcagggc atcaacattc cctacatcac cacacttcat
      841 ggaacagaca tcaccctttt gggaaaggat gaatcgtttg agcctgtaat tacttttgcc
      901 attaacgaaa gcgatgctgt aacagctgta tcggaaagtt tgcgtcagga cacactaaaa
      961 ttttttcgtg taaaaagaga tattaaggta atacccaact ttatttgtga ggaagaatat
     1021 aatgtgcctg tcctcgaaaa tctgaaacgg tcttatgctc ccaatggtga gtttatcctc
     1081 acacatattt caaatttcag accagtgaaa cgggtggatg acgttgttcg ggtttttgag
     1141 ctggttcgta agaaacttcc ttccaaacta ttgctggtgg gagatggtcc ggagcgctca
     1201 catgtagaaa aaatgtgcag ggaatcagga cttggtactg atgtgatttt cgttggaaag
     1261 atgaagcaaa c
//
LOCUS       DAOMYG010000596         1252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_44847_length_1252_cov_10.538012, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000596 DAOMYG010000000
VERSION     DAOMYG010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44847_length_1252_cov_10.538012"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PK610_14045"
     CDS             complement(<1..998)
                     /locus_tag="PK610_14045"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRJ37113.1"
                     /translation="MKKYLLLLSFLEGASVMAAELMGAKMMAPFFGSSLYVWSAVMAI
                     TLGGLAAGYFAGGILSTKKNPEQWLYKVLVLAAALTALMPFTSKFSLSLFGYFSLLPA
                     VSINAMLILFPPVFLMGTVSPLIIRNIATDGEHAGKAAGTVYAISTVGGILATFLFGF
                     WIIPTFGLTYPAIATGIVLGILPAIELVKGKKLPAVLFLGACIFLAARTTAVKPVSSI
                     RVVHSGEGVLGQIMVLDFPNDVYYADTTKAGQTSRWLFVNRVSQTMDDPHADRNKGEE
                     RYFTYIYKLEQVLDTFSSHRKNILLLGLGGGSVAKHFSEKGFSVTACELDPRIAQVA"
BASE COUNT          355 a          287 c          309 g          301 t
ORIGIN      
        1 gccacttgtg caatgcgcgg atccaattcg caggctgtaa cggaaaatcc tttttcggaa
       61 aaatgtttag ccacgcttcc tcctcccaaa ccgagtaaca agatgttttt gcggtgcgag
      121 gaaaaggtat cgagcacttg ttcaagtttg tagatgtaag tgaaataccg ctcttctcct
      181 ttgttgcgat cagcatgggg atcgtccatg gtttgcgata cgcggttcac gaacaaccac
      241 ctgctggtct gacctgcttt ggtggtgtcg gcatagtaca catcattggg gaaatcgagc
      301 accataatct gcccgagcac tccttcgccc gaatgcacca cacggatgga gcttaccggt
      361 ttcacggctg tagttcgagc tgcaagaaaa atacaggcac ccagaaatag aacggcaggt
      421 agttttttcc ccttcaccaa ttcaatcgcg gggagaattc ccaatacgat tcccgttgcg
      481 atggcaggat aggttaaacc gaaagtggga atgatccaga aaccaaaaag aaaagtggca
      541 agaatgcctc ccacggtgga aatggcgtac acggtgcccg ctgcttttcc ggcgtgttct
      601 ccgtcggtgg caatgttgcg gatgatgagc ggagaaaccg ttcccatcag aaataccggt
      661 ggaaaaagaa tcagcatggc gttgatgctg actgcaggta gaagagaaaa atatccgaag
      721 agcgacaatg aaaatttact ggtaaagggc atcaatgcgg tgagtgctgc tgccagaacg
      781 agcactttgt acaaccactg ctccggattt tttttcgtgg ataaaatccc tcccgcaaaa
      841 taacccgctg ccagtccgcc cagggtgatt gccattacgg ccgaccatac atacaaggag
      901 gaaccaaaaa acggtgccat catttttgcg cccatcaatt ccgccgccat aacggatgcg
      961 ccttcaagaa aggaaaggag taaaaggtat tttttcattg ggacgaagat aggggggaat
     1021 acctctcaga cgctcaggat agggataatc atcacaattg catagctgaa actatttgaa
     1081 aaacaatcga ttgtaggtaa tctatgcaaa ttcggaattc gattccgaat ttgcatagat
     1141 tttgtatatt tgtgtatgat ttatagggat ttatcaaatg ttttgaaggg actctccggt
     1201 aaaatgccca tcctatctgt cacggggcca cgtcaatcgg gaaaaacgac cc
//
LOCUS       DAOMYG010000597         1244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_45283_length_1244_cov_11.564340, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000597 DAOMYG010000000
VERSION     DAOMYG010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45283_length_1244_cov_11.564340"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..>1244)
                     /locus_tag="PK610_14050"
     CDS             complement(4..>1244)
                     /locus_tag="PK610_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRJ37114.1"
                     /translation="HRLSGSAQAEKAVVWGKQTMEKAGFDQVWLQDVMVPKWVRGKEK
                     GSLLLSKNPKLKDGNPVSATLKITALGGSVGTNGPITGEVIEVNDFDELKRLGTENIK
                     GKIVFFNRPFDPTFIQTFKAYGHCVNQRVQGASEAAKFGAKAVIVRSMAHGHGDHPHT
                     GSIHYEDSLKKIPAAAVSTVMADKISDELKQGRKVSVTLEMDCKVFPDVLSHNVIGEL
                     KGSEFPDRIITIGGHLDSWDIGEGAHDDGAGCVQSIEVLRLFKSLGIRPRHTLRAVLF
                     MNEENGLRGGKKYADEAERKGEIHVAGLESDRGGFAPRGFTIEGNEQQIAWLRSLNPL
                     LKPYDLHFFEPGGSGADIGPLKKKFSNIVLFGFIPDSQRYFDHHHAETDVFETVNKRE
                     LELGAAAMATLIYLIDRNLL"
BASE COUNT          333 a          301 c          234 g          376 t
ORIGIN      
        1 cacttaaagt aaattgcgat cgatcaaata gatcaaggtt gccatggcgg ccgcacctaa
       61 ttccagttct cttttgttta ccgtctcaaa aacatcggtt tctgcatggt ggtgatcaaa
      121 ataccgttgg gagtcgggga taaatccgaa taagacgatg ttggagaatt tcttttttaa
      181 aggaccaata tcggctccac ttccgccggg ttcaaaaaaa tggagatcat agggttttag
      241 caatgggtta agcgaacgaa gccaggcaat ttgttgctca ttgccctcaa tagtaaatcc
      301 gcgtggagca aatcctcccc gatcactttc gagtcctgca acatgaattt cacctttgcg
      361 ttcagcttca tctgcatatt tttttcctcc ccgaagtccg ttttcttcat tcataaataa
      421 aacagcccgc aaggtatgac gtggacgtat cccaagtgat ttaaataaac gtaacacttc
      481 aatggactgt acgcatcccg ctccatcatc gtgtgcgcct tctcctatgt cccaactgtc
      541 aaggtggcct ccaattgtaa tgatgcgatc cggaaattcg ctgcctttta gttctccaat
      601 gacattatgc gataacacat caggaaatac tttgcaatcc atttcaagag taacagaaac
      661 ctttctgcct tgcttgagtt catctgaaat tttatctgcc attacagtgg aaacagcagc
      721 agcaggaatt ttctttaaag aatcttcgta atgaatacta ccggtatgcg gatgatcacc
      781 atgaccatga gccatacttc gcactataac agcctttgct ccaaacttcg ctgcttctga
      841 tgcgccttga acgcgttgat ttacacaatg accgtatgct ttaaatgttt gaatgaaagt
      901 gggatcgaaa ggtcggttga aaaagacaat tttccccttg atgttttcag ttcctaatcg
      961 tttcagttca tcaaaatcat tgacttcaat aacttcaccc gtaattgggc catttgttcc
     1021 aacagaacct cccagtgcag tgatctttaa tgtagcactt acgggattgc catctttcaa
     1081 tttgggattt ttacttaaaa gcagagatcc tttttctttt ccgcgaaccc atttgggtac
     1141 catcacgtcc tgaagccaaa cctgatcaaa tcctgccttc tccatagtct gttttcccca
     1201 gacaaccgct ttttccgctt gtgctgaacc gctcaaacga tgtc
//
LOCUS       DAOMYG010000598         1235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_45775_length_1235_cov_11.519492, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000598 DAOMYG010000000
VERSION     DAOMYG010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45775_length_1235_cov_11.519492"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..>1235
                     /locus_tag="PK610_14055"
     CDS             58..>1235
                     /locus_tag="PK610_14055"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRJ37115.1"
                     /translation="MRSDTIDVINYSIALDIRDFAGQTISGSTTVKFRPLMNGVTSLS
                     LDLLEMTVDSVKKNSTNLSFTYNDTLLVIDLSLSIGIGDTSQLTVYYHGQPQMDPSGW
                     GGFYWNGGYAFNLGVGFESIPHNYGRVWHPCFDNFVERATYDFVILTNGGKRAYCNGY
                     FVNETVNGSDITRTWKMETPIPSYLASVNVASYTHVVQNYNSPFTGLNTPIWLIALPA
                     DTTGMKNSFVNLPTAIESFEWAYGPHYFNKIGYVLVPFSSGAMEHATNIAYPRTTATG
                     SLLYETLMAHELAHHWWGDLVTCETAYDMWINEGMASYSEYLFLEYMYGETQYKNGIR
                     NNHKNVIWKAHIDDNGFHALSQVPLDVTYGATSYDKGADVAHTMRGYMGDSLFFAGKM
                     "
BASE COUNT          361 a          235 c          268 g          371 t
ORIGIN      
        1 gctcaacatt gttctcacaa gggagcaaaa tatcatgatc gtacatcaaa cgaaaatttg
       61 cgcagcgata caattgatgt gatcaattat tcaatcgcac ttgatattcg tgattttgca
      121 ggacaaacta tttccggatc aacaactgta aagttcagac cgctgatgaa tggggtcaca
      181 agtttgtcgc ttgacctttt ggagatgaca gtggattcgg taaagaagaa ttcaacaaac
      241 ctttcgttca cctacaatga tacattattg gtaattgatc tcagtttgtc aattggaatt
      301 ggtgatactt cacaacttac tgtgtattat catgggcagc cacaaatgga tccttcagga
      361 tggggagggt tttattggaa cggtggttat gcgtttaatt taggtgtagg atttgaatct
      421 attccacata attatggacg tgtatggcat ccttgtttcg ataattttgt tgaacgtgca
      481 acctatgatt tcgtgattct taccaatgga ggaaaaagag catactgtaa cggatatttt
      541 gtaaatgaaa ccgtaaatgg ttcagacatt acacgtacat ggaaaatgga aacacctatt
      601 ccaagttatc tcgcctcagt aaatgttgct tcgtacacgc atgttgttca gaattataat
      661 agtccattta ccggattaaa cacccccatt tggctgatcg cacttcctgc cgatacaaca
      721 ggcatgaaga attcatttgt aaatcttcca accgcaattg aatcatttga gtgggcgtat
      781 gggccccatt attttaataa gattggttat gttctggtgc cattctcatc cggagcaatg
      841 gagcatgcaa ccaatatagc ctatccccga actactgcaa ccggttcctt gctttatgaa
      901 acgctaatgg ctcatgaact cgcgcatcat tggtggggcg acctggttac ctgcgaaact
      961 gcatacgaca tgtggatcaa tgaaggaatg gcatcgtatt ctgaatactt gtttctggaa
     1021 tacatgtatg gagaaacgca atacaaaaac ggtattcgca acaatcacaa gaatgttatt
     1081 tggaaagcac atattgatga taacggtttt catgcgttgt cgcaggtgcc gcttgatgtt
     1141 acttacggag caacttccta tgataaaggt gcagatgttg cacatacaat gcgtggatac
     1201 atgggagatt ccctgttttt tgccgggaaa atgag
//
LOCUS       DAOMYG010000599         1205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_47488_length_1205_cov_9.008696, whole genome shotgun sequence.
ACCESSION   DAOMYG010000599 DAOMYG010000000
VERSION     DAOMYG010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47488_length_1205_cov_9.008696"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PK610_14060"
     CDS             complement(<1..311)
                     /locus_tag="PK610_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37116.1"
                     /translation="MKKTIFLLLSFFAGSQTQAQFSATHNHTEHNLSHSFQKAYMNHP
                     SVPKGILEAVAWTSTRMMHLDGAEEESCIGYPRAYSVMGLTLNGQSYFRENLKLVSNL
                     SG"
     gene            complement(498..1139)
                     /locus_tag="PK610_14065"
     CDS             complement(498..1139)
                     /locus_tag="PK610_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37117.1"
                     /translation="MRSMQPTFSIRSLFFLISILFSVSATAQEEGNPDYVEYKKQKED
                     EKKKEKTELSEYSFAERCYWGGSLSLSLGNYGSYIDISPILGYNLTMKWSAGIGISYK
                     YFGGRNQFYGVTYSTSVYGGCVFTRLLMGEKFFAHAELESLNTDAYDPLTLQFKRKFI
                     NIGAAGLGLRNEMGYNRYFYFMLLYDFINDRDSPYPFSPFIVKAGAIFPIADR"
BASE COUNT          355 a          227 c          264 g          359 t
ORIGIN      
        1 ccggataagt ttgaaaccag ttttaaattc tctctgaaat atgattgtcc atttaacgtt
       61 aatcccatta cggaataggc acgtggataa ccaatgcaac tttcttcttc tgcaccatcg
      121 agatgcatca tacgggttga tgtccatgca actgcttcca ggataccttt tggaaccgaa
      181 ggatgattca tgtaagcctt ttgaaagcta tgtgaaagat tatgttcggt gtgattgtgt
      241 gttgcgctga attgggcttg tgtttgactt cctgcaaaaa aagaaagaag tagaaaaatg
      301 gtttttttca tatcgcgtgc taagtagtag ggtaaaaata gaatcgaata ctacaataca
      361 caagcaaaat tggaatgttt ttgcggaatg aaaaatgaaa cggtttaggt gtgagaataa
      421 aacaagactg ttaattttta ctgcaattca atgtgtccgg aaagcatgct tttctggtag
      481 tttgcaagaa ctcttgctta gcggtctgca atcgggaaaa ttgctcctgc tttcacaata
      541 aagggtgaaa aaggataagg ggaatcgcgg tcgttgatga aatcgtaaag caacatgaaa
      601 tagaaatacc tgttgtatcc catttcattt cggagtccta gtcctgccgc accgatattg
      661 atgaactttc gcttgaactg tagtgtaagc ggatcgtaag catcggtatt caggctttcc
      721 agttcggcat gagcaaagaa tttttccccc atcaataggc gggtaaatac acacccacca
      781 taaaccgagg ttgaataggt cactccgtaa aattggtttc ttccgccaaa atacttgtac
      841 gagatcccaa ttccggccga ccatttcatt gtgagattgt atcctaaaat gggtgatata
      901 tcaatgtagg aaccgtagtt tccaaggctg agcgataagc taccgcccca ataacagcgc
      961 tcggcaaatg aatattccga gagttctgtt ttctcttttt tcttttcgtc ttccttttgt
     1021 tttttgtatt caacgtaatc gggattaccc tcctcctgag cagttgcact aacggaaaac
     1081 agaatgctga tcaaaaaaaa cagcgatcga atggaaaacg ttggctgcat actgcgcatg
     1141 gcttttggag gtgagcgttt tggattgaga cggtaaagat attatctgtt gaacaaaatt
     1201 cgtac
//
LOCUS       DAOMYG010000600         1178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_49201_length_1178_cov_7.141585, whole genome shotgun sequence.
ACCESSION   DAOMYG010000600 DAOMYG010000000
VERSION     DAOMYG010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49201_length_1178_cov_7.141585"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="PK610_14070"
     CDS             <1..892
                     /locus_tag="PK610_14070"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRJ37118.1"
                     /translation="YNRYRLKQKANEQLQSYNDQLKQFNDEITHQKEIIEEKNRDIMD
                     SIRYAKRIQEAILPAHDLMSAHIRDFFVLYRPKDIVSGDFYWSLPIQENGRNKILFAA
                     VDCTGHGVPGAFVSIVGYNGLNRAVKEFHLYEPSKILDKLTELVEDTFKQQGSSNIKD
                     GMDITLCSLEYLDNETAVLKFAAANNPLWIFRNGTEWEEVKADKQPIGAFDDRKPYTQ
                     HEFILSKGDTFYIFSDGYPDQFGGDKGKKYKYSKFRDYLQAIIPHNMEKQKELLSEEI
                     VAWTGDLEQVDDQCVIGIRI"
     gene            922..>1178
                     /locus_tag="PK610_14075"
     CDS             922..>1178
                     /locus_tag="PK610_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018945328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37119.1"
                     /translation="MKKDPGLWNKIERLELDDPQADLTFSGRLARENGWSLEFALGAI
                     EEYKRFIYLICVSNTALTPSDEVDQVWHLHLLYTESYWDEL"
BASE COUNT          383 a          237 c          258 g          300 t
ORIGIN      
        1 gtacaaccgt tatcgcttaa agcaaaaagc aaacgaacaa ttgcaatcct acaacgatca
       61 gctgaaacaa ttcaatgatg agattacgca tcaaaaggaa atcatcgaag aaaagaaccg
      121 cgacatcatg gattctattc gttatgcaaa gagaattcaa gaagcgatac taccggcaca
      181 cgatttaatg tcggctcata ttcgcgactt ttttgtattg tacagaccaa aggatattgt
      241 ctccggcgat ttttattggt ccttacccat acaggaaaat ggcaggaata aaattttatt
      301 tgccgctgta gattgcaccg gccatggcgt accgggagca tttgtttcca ttgtaggcta
      361 caacggattg aaccgcgcag taaaggaatt ccatttgtac gagccctcca aaatccttga
      421 caagctcacc gaattggttg aagatacgtt taagcaacaa ggaagcagta atatcaaaga
      481 cggaatggac atcacacttt gttccctcga atatttggat aatgaaacag cagttttaaa
      541 attcgccgct gcaaacaatc cgctttggat tttccgaaat ggaacagaat gggaagaagt
      601 gaaagccgat aaacaaccca ttggagcatt cgacgacaga aagccgtaca cgcaacacga
      661 attcatctta tcaaagggcg atacttttta cattttctca gatggttacc cagatcaatt
      721 tgggggcgac aaagggaaaa aatacaaata ctccaagttc cgcgactatc tgcaagcgat
      781 aattccgcat aatatggaga aacaaaaaga actcctttcc gaagaaatag ttgcctggac
      841 aggtgacctt gaacaagtgg acgaccaatg tgttattggt ataagaatct gaattttccc
      901 ttgtaaaaag ctggctggtc tatgaaaaaa gatccgggtt tatggaataa aattgagcga
      961 ttggaactcg atgatccgca ggctgatctc actttctccg gaagactggc aagagagaac
     1021 ggatggtcgc tggagtttgc tttgggagca attgaagaat acaagcgatt catttacctg
     1081 atctgtgtaa gtaataccgc attaacgccc tctgatgaag tggatcaggt gtggcatctt
     1141 catttactct ataccgaatc gtattgggac gagctttg
//
LOCUS       DAOMYG010000601         1161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_50300_length_1161_cov_9.339060, whole genome shotgun sequence.
ACCESSION   DAOMYG010000601 DAOMYG010000000
VERSION     DAOMYG010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50300_length_1161_cov_9.339060"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1161
                     /locus_tag="PK610_14080"
     CDS             <1..>1161
                     /locus_tag="PK610_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRJ37120.1"
                     /translation="GGEKPLFSSDGKRIYFFGSGFPNVEYKSVDLNGNDEQLHFTSRY
                     ATDFVPSPDNRWIAFRELHNVYVAAFPASGKAIDLSAQAKSVPLAKVSRDAGINMHWS
                     GDGKVLHWMLGEEYFSVPLDQKFDFLRNGNPAKAINDSTGIRVGLQLKTDRPEGRIAF
                     KGAKIITMNGNEIIESGTIIINGNRVENMGKDADVTIPSGCKVIDAKGKVIIPGFVDV
                     HAHVGHFRQGLTEDKHWQYYANLAYGVTTTHDPSTNTEMVFAYSELVRSGLMVGPRVF
                     STGTILYGADGDFKAPINSIDDARSAIRRTMAFGAFSVKSYNQPRREQRQMVIQAARE
                     QLCMVVPEGGSTFYHNMTMIADGHTGVEHNIPVAPAYNDVIQFWKNSATGYT"
BASE COUNT          330 a          215 c          289 g          327 t
ORIGIN      
        1 ccggtggaga gaaaccactt ttttcatcgg atggaaagcg gatttacttt tttggtagtg
       61 gatttcccaa cgtggaatac aagagtgttg acctgaatgg aaatgacgag caattgcatt
      121 ttacttcgcg ttacgcaaca gattttgttc ctagtcctga taatcgctgg attgcgtttc
      181 gtgaattgca caatgtgtat gtggctgcat ttcctgcaag cggtaaagcc atcgacttga
      241 gtgctcaggc aaaaagtgtt ccgcttgcaa aagtttcacg tgatgcagga atcaatatgc
      301 attggagtgg cgatggaaag gttctgcact ggatgttagg agaggaatat ttttctgttc
      361 cgctcgatca gaaattcgat ttccttagaa atggaaatcc tgcaaaagcg attaatgatt
      421 caaccggcat tcgagtcggt ttacaattga aaacagatcg accggaaggg agaattgcat
      481 ttaaaggagc aaagatcatt actatgaacg ggaatgagat cattgaaagc ggaacaatta
      541 ttatcaacgg aaaccgggtc gagaacatgg gcaaagatgc agatgttacg atcccttcgg
      601 gatgcaaggt gattgatgcg aaaggaaaag tgatcattcc cggatttgtt gatgttcatg
      661 cgcatgtggg tcattttcgt caagggctaa ctgaagataa gcattggcag tattatgcaa
      721 atctcgccta cggtgttact actacacacg atccgtcaac gaatactgaa atggttttcg
      781 cttattcaga attggtgaga tcaggattaa tggttggtcc gcgtgttttt tctacgggta
      841 ctattctata tggagccgat ggcgatttta aagcacctat taattcgata gatgatgccc
      901 gttctgcaat acgacgtaca atggcatttg gtgcgttcag tgttaaaagc tacaatcaac
      961 cccggcgcga gcaacgacaa atggttattc aggcagcacg tgaacaattg tgcatggttg
     1021 ttccggaagg tggatcaaca ttttatcata acatgacgat gatcgcagat ggacacaccg
     1081 gtgttgagca taatattccg gttgcacctg catacaacga tgtcattcag ttttggaaaa
     1141 attctgctac tggttacacg c
//
LOCUS       DAOMYG010000602         1137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_52003_length_1137_cov_6.439002, whole genome shotgun sequence.
ACCESSION   DAOMYG010000602 DAOMYG010000000
VERSION     DAOMYG010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52003_length_1137_cov_6.439002"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..>1137
                     /locus_tag="PK610_14085"
     CDS             158..>1137
                     /locus_tag="PK610_14085"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRJ37121.1"
                     /translation="MFLIFDTETTGLPQDYNAPIENTSNWPRVVQIAWQLHDEKGALL
                     EVKNFIIKPVGYSIPYNAEKIHGISTDRALKHGVDIAFAFEEFQKALERTTFIAGHNI
                     EFDIKVWGAEYVRSGLALELLKKKTLDTKEEATDFCAIPGGKGGRFKWPTLTELHKKL
                     FGTGFEAAHNASADVEATTRCFLELIRLNVITPNRMGLSMDFLHAFLQNNPGPIQAIG
                     LNIQPYEPIQSEAQDLFQQEDTISNSTAVDHSELLKEIPFSHLHNHTQYSILQSTTDV
                     GKLVKKAGQSGMPGLGLTDTGNMMAAFDFVKEVFSYNKGVEARKKEAEAKG"
BASE COUNT          356 a          235 c          239 g          307 t
ORIGIN      
        1 gccggcacca attaccaatg catccatttt aaatgttttt ttgcccaaag ttaagggtga
       61 tcctcaaact gtcaataaac cgtgcattta tcaacaaaaa tccattccgg gcaatggatt
      121 ccgtaatttt cgagtctaat tcccaattcc acgctacatg ttcctcatat ttgataccga
      181 aacaacagga ttaccacagg attataatgc tccaattgaa aacacttcca attggccgcg
      241 agtagttcag attgcatggc aattacacga cgaaaaggga gcattacttg aggtaaagaa
      301 tttcataata aaacctgttg ggtattccat cccctataat gccgaaaaaa ttcacggtat
      361 aagtacggac agagcgctta aacacggggt agatattgcg tttgcgtttg aagagtttca
      421 gaaagcactt gagcgtacaa cattcattgc aggacacaat attgaattcg acattaaagt
      481 ttggggagca gaatatgtac gttccgggtt agcccttgaa ttactaaaaa agaaaaccct
      541 tgatacaaag gaagaggcaa ccgatttctg tgcaatcccg ggaggaaaag gtggtcgctt
      601 taaatggcct actttaacag aacttcacaa aaaattattc ggtacaggtt ttgaagctgc
      661 gcacaatgca tctgctgacg tggaagcaac aacccgatgt ttccttgagt tgatccgcct
      721 caatgtaatt actccgaatc gcatggggtt gagtatggat tttcttcatg cttttttaca
      781 gaataatcct ggtcctattc aagcaatcgg gctcaacatc cagccttatg agcccattca
      841 gagtgaagct caggatttgt ttcagcaaga ggatacaatc agcaattcaa ctgctgtcga
      901 tcacagtgaa ttattaaagg aaattccatt ttctcatctg cacaatcata cacaatattc
      961 tattcttcag tcgaccaccg acgttgggaa actggtgaaa aaagccggtc agtcagggat
     1021 gccgggttta ggtctgactg ataccgggaa tatgatggct gcattcgact ttgtgaagga
     1081 agttttttcc tataacaaag gtgttgaagc acgcaaaaaa gaagcagagg ccaaagg
//
LOCUS       DAOMYG010000603         1123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_53013_length_1123_cov_11.296816, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000603 DAOMYG010000000
VERSION     DAOMYG010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53013_length_1123_cov_11.296816"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="PK610_14090"
     CDS             <1..751
                     /locus_tag="PK610_14090"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRJ37122.1"
                     /translation="AMTGLDQNMMQKNLSCKSLGEAQKNIRTFSFVMMIVNIFFLALG
                     VLLYQYYTTAEITLPLAANGDVLTDKVFPKLAMEHLGMFPGIVFILGLTAATFSSADS
                     VLTTLTTSFYIDILRMDKNTAKSEASKHRIRNIIHISFAFTLLIVILIFDAMNNDAII
                     NTILTIAGYTYGPLLGLFAFGIYTTRIAKDNWVPVICILAPLLSWLLSTYSKTILGGY
                     EFGYELLGVNGLLVFAGLMLVSKPKSTTAIV"
     gene            complement(731..>1123)
                     /locus_tag="PK610_14095"
     CDS             complement(731..>1123)
                     /locus_tag="PK610_14095"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37123.1"
                     /translation="APSEARILIDGTEYKKKIMTIVVANGKGFGNGLIIAPDSKLDDG
                     KFDIIIIGNVGVFQYLKYISRIRKGEKIIHPEVIYLSGKQLSIEPVGHSLMVEYDGEF
                     GGYAPASVEMHRSAIKLLIPVESNNSGR"
BASE COUNT          320 a          243 c          207 g          353 t
ORIGIN      
        1 tgccatgacc ggtcttgatc agaacatgat gcaaaaaaac ctcagttgca aatcgcttgg
       61 agaagctcaa aaaaacatcc gcacattcag ttttgtaatg atgattgtaa atattttctt
      121 tctcgctcta ggtgttttgc tttaccagta ttataccact gcagaaatta cattacccct
      181 tgcagcaaat ggagatgtat taaccgataa agtatttcca aaacttgcga tggaacattt
      241 aggtatgttt ccgggaattg ttttcattct tggtttaaca gcagcaacct tttcgagcgc
      301 agattctgtt ctcaccacct taacaacttc cttctacatt gatattcttc gcatggataa
      361 gaatactgca aagagcgaag catccaaaca ccgcatcaga aacatcattc atatttcatt
      421 tgcctttacg ctgttgattg tgattctcat ttttgatgca atgaacaacg atgctatcat
      481 caataccatt ctcacaattg caggatatac ctacggacca ttacttggac tttttgcatt
      541 tggaatttac accacacgca ttgccaagga caattgggtt cctgtcatct gtattcttgc
      601 tccactgctg tcgtggttgc ttagcaccta ttccaaaact atattgggag gatacgaatt
      661 cggctatgaa ttattgggag taaacggact gcttgttttt gcaggattga tgttggtatc
      721 gaaacccaaa tcaacgaccg ctattgtttg attccacagg aatcagcaat ttgattgcag
      781 aacggtgcat ttcgacggaa gcaggcgcat agccaccaaa ttcaccatcg tattcgacca
      841 tcagggaatg tccgacaggt tcaatcgaca attgttttcc actgaggtaa atgacttccg
      901 gatgaataat tttttctcct ttgcggatgc gggaaatgta tttcaggtat tgaaaaactc
      961 ccacgtttcc gataatgata atatcaaatt tcccatcatc cagttttgaa tccggagcaa
     1021 taatcaatcc gttaccaaaa ccttttccgt tggcaactac aatggtcatt atcttttttt
     1081 tgtattcagt accatcaatc agaatgcgtg cttcggaagg agc
//
LOCUS       DAOMYG010000604         1112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_53815_length_1112_cov_11.333018, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000604 DAOMYG010000000
VERSION     DAOMYG010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53815_length_1112_cov_11.333018"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..>1112
                     /locus_tag="PK610_14100"
     CDS             56..>1112
                     /locus_tag="PK610_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ37124.1"
                     /translation="MNILAPGDMGSTFWAKIPPAATATCNSSPRFNALPPLVMCNGSA
                     FSFDHSATDPDGDQLQYVFYTPFHGGDAFAPAPNPAFPPPYTTINWAGGFSLGNQIQG
                     APAFNLNAGTGLLTGTPITNGIHLYGVKVIERRNGQIISEIFREFQITVTNCPSLVVS
                     AIPVQTELCQGMTLNIGNNSQNSSFYHWDFGVPGITNDTSNQVAPTYTYPDTGTFTIT
                     LIANPGVWCADTATQTYEVHLPLNLSLATPAPQCEDVNSFDFDLNGNFTNAANIQWTF
                     GPFANPSTSAVADPQNVVYSDSGHYNASVIVTQFGCTETVDVDLVVFPLPEFGFTYPP
                     DDGCAPFPVFFTNTSYAW"
BASE COUNT          266 a          305 c          219 g          322 t
ORIGIN      
        1 ttcagccatc ggatacgata tcgtttatca acgctgctgt cacaatccca atatcatgaa
       61 catcctcgca ccgggtgata tgggatctac gttttgggcg aagattccgc ctgctgcaac
      121 ggcaacctgt aacagcagtc cgcgcttcaa tgcacttcct ccgttggtaa tgtgcaacgg
      181 ttccgcattt tcgttcgatc actccgctac cgatcccgat ggcgatcagc tccaatatgt
      241 tttttatact ccatttcatg gtggcgatgc ttttgctcct gctcccaatc ctgcatttcc
      301 tcctccctac accaccatca actgggcagg tggatttagc ctgggcaatc aaattcaagg
      361 cgcacctgca ttcaatctga acgcgggaac cggattgctc accggaactc ctattacaaa
      421 cggaattcat ttgtatggag taaaagtgat tgaacgaaga aacggacaaa tcatctcaga
      481 aattttccgc gaattccaga tcaccgttac gaattgcccc tctcttgttg tttctgctat
      541 tcctgtgcaa actgaattgt gtcagggtat gacactcaac attgggaata acagtcaaaa
      601 ctccagtttc taccattggg attttggtgt tccaggcatt acaaacgata cttccaatca
      661 agttgcacct acctacactt atcccgatac cggaacattt accattacgc tcattgccaa
      721 tccgggtgta tggtgtgcag ataccgcaac gcaaacctac gaagttcatt taccgcttaa
      781 tctcagtctg gctactcctg cgccgcaatg tgaagatgtg aattccttcg atttcgatct
      841 caatggaaat ttcaccaatg cagcaaacat ccagtggaca ttcggcccat ttgcaaatcc
      901 ctctacttcc gccgttgccg atccgcagaa cgtggtgtat tccgattccg gtcattacaa
      961 tgcatccgtt atcgttaccc aattcggctg taccgaaaca gtggatgtag atcttgttgt
     1021 ttttcctctt cctgaattcg gttttaccta tccgcctgat gacggctgcg ctccttttcc
     1081 cgtatttttc accaatactt cgtacgcatg gg
//
LOCUS       DAOMYG010000605         1101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_54617_length_1101_cov_6.963671, whole genome shotgun sequence.
ACCESSION   DAOMYG010000605 DAOMYG010000000
VERSION     DAOMYG010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54617_length_1101_cov_6.963671"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PK610_14105"
     CDS             complement(<1..197)
                     /locus_tag="PK610_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37125.1"
                     /translation="MKSFFENILKDKHGMFSLRETVVAIFMVATLIAWAGEQFFLLPC
                     PEHIFYGFISLIAAGCNEGNEA"
     gene            complement(201..410)
                     /locus_tag="PK610_14110"
     CDS             complement(201..410)
                     /locus_tag="PK610_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37126.1"
                     /translation="MKDTPTVENNSNEFFDTLLSIAQQSLNQPQPPYSVCINQSRNLN
                     NIKVDIHVVNTPQPGFFRKLFSKLF"
     gene            complement(416..1096)
                     /locus_tag="PK610_14115"
     CDS             complement(416..1096)
                     /locus_tag="PK610_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010181381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37127.1"
                     /translation="MARKNKYVIYDKPYQLNIWGIRSPQTRSGQFDDHFHVFYKDGNG
                     RWEYHIFRCTTDPGTFWLHDPMRPEGTAILAQGQYIDAYQIGIHYTYAALVQRKPVRV
                     IRDYNRDSVLDFYNGNYHTGLFGINIHRAMMYGKTPYIEKFSAGCQVFENASDFYHFM
                     QMAERHRSLYGNRFSYTLIDERAIKRAKLRRTFYGALAVGASTATLLYLLTSSENQEN
                     QNQSTLTN"
BASE COUNT          296 a          215 c          276 g          314 t
ORIGIN      
        1 gcttcatttc cctcattgca tccggctgca atgagggaaa tgaagccgta aaaaatatgt
       61 tcgggacaag ggagcaggaa aaactgttcg ccagcccacg cgatcagggt agccaccatg
      121 aagatggcca ccactgtttc gcgcagggaa aacattccgt gcttgtcttt gagaatattt
      181 tcaaagaagg atttcatgtt ttagaaaagt ttggaaaaca gttttctaaa aaatcccggt
      241 tgcggagtat tcacaacatg aatatccact ttgatgttat tcaaattcct gctttggttg
      301 atgcaaaccg aatacggtgg ttgcggttga ttcagggatt gctgtgcaat ggacagcagg
      361 gtgtcaaaaa actcgttgga gttgttttct acagttggtg tgtctttcat aaattttaat
      421 ttgttaatgt tgattgattt tggttttctt ggttttcaga agaggttaag aggtaaagca
      481 gcgtggcggt ggaggctcct acagccaatg cgccataaaa agttctgcgt agcttggcac
      541 gtttgatggc acgttcatct atgagcgtat aactaaagcg attaccgtag agggaacggt
      601 gtcgctcggc catttgcata aagtggtaaa aatccgatgc gttttcaaat acctggcacc
      661 cggcagagaa cttttcgatg tagggagttt ttccatacat catagcgcgg tgaatgttga
      721 tgccgaaaag tcctgtgtgg taatttccgt tgtaaaaatc caataccgaa tcccggttgt
      781 agtcacggat taccctgaca ggttttcgtt gcactaaggc tgcgtaagtg taatgaatgc
      841 cgatttgata agcatcgatg tattgcccct gcgcgagaat agcggttcct tcaggtcgca
      901 tagggtcatg cagccaaaaa gtaccgggat cggttgtgca gcggaaaata tgatactccc
      961 aacggccatt gccgtcttta tagaatacat gaaaatgatc gtcaaactgc ccggaacgcg
     1021 tctgcggtga acgaataccc caaatgttta actggtaggg tttatcgtaa atcacgtact
     1081 tgttcttgcg agcaattgat t
//
LOCUS       DAOMYG010000606         1095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_55089_length_1095_cov_9.027885, whole genome shotgun sequence.
ACCESSION   DAOMYG010000606 DAOMYG010000000
VERSION     DAOMYG010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55089_length_1095_cov_9.027885"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1095
                     /locus_tag="PK610_14120"
     CDS             <1..>1095
                     /locus_tag="PK610_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRJ37128.1"
                     /translation="GPRVTAEGFYCFINATIYVSADKKLDNASLVIRRGRIEDVGTGI
                     TIPKGAVVVDLAGKHVYPSFIDLHTSYGLAETARQQGGGYRGPQYESSKKGAYYWNES
                     IHPEYRAAEVFKADPKVSAEYRSAGFGAVLSHLQDGIARGTSVFTTLSDKRENESVLI
                     TDAAQHFSFRKGNSNQAYPSSLMGSIALLTQTYYDAKWYAAQKQLKEENLSLQALNRW
                     HNLPQIFDANDKYNVLRADKIGDQFGVQYVFKTNGKEYQRLNEIKSSKGTLIVPLNFP
                     DPYDVEDTYDAMNVSLEDMKHWELAPYNPSLLHKSQIRFVLTSSDLKEKKQFLPNLRK
                     AVRYGLPEAEALKALTVYPAQLLGVDKQLGT"
BASE COUNT          339 a          226 c          237 g          293 t
ORIGIN      
        1 tggtccacgg gttacagccg aagggtttta ttgttttatc aatgctacga tctatgtctc
       61 ggccgataaa aaactggata acgcttcact cgtaattcga agaggacgaa ttgaagatgt
      121 tggaacagga attaccattc ccaaaggagc ggtagttgtc gatcttgccg gaaagcatgt
      181 ttatccctcc ttcatcgatc tccatacatc ttacggactg gcagaaacag ctcgtcagca
      241 aggcggagga taccgcggac ctcaatatga aagttcaaaa aaaggagctt attactggaa
      301 tgaatccatt catcctgaat acagggcagc agaggttttc aaggcagacc caaaagtttc
      361 agcagagtat cgatcggcag gattcggagc ggtattatcg catttgcagg atggaattgc
      421 acgcgggact tcagttttta caacattatc ggacaagcgt gagaacgaaa gtgtattaat
      481 taccgatgcg gcacaacatt tttcatttag aaaagggaat agcaatcaag cctatccatc
      541 ttcattaatg ggcagcattg ctcttctcac ccagacttat tacgatgcaa agtggtatgc
      601 cgctcaaaag caattgaagg aagagaattt atcattacaa gcacttaacc gctggcataa
      661 ccttccacaa attttcgatg caaatgataa atacaatgtg ttgcgtgcag ataagatcgg
      721 agatcagttt ggtgtacagt acgtatttaa aactaatgga aaagaatatc agcgtctcaa
      781 tgaaatcaag tcttcgaaag gtactttgat tgtaccactc aattttcccg acccctatga
      841 tgtagaggat acttacgatg cgatgaatgt ttcgctagag gacatgaagc attgggaact
      901 tgctccatat aatccatcat tgcttcataa atcgcagatc cgatttgtgc ttacctcttc
      961 agatctgaaa gaaaaaaagc agtttttacc caatcttcgt aaagcggttc gctacggatt
     1021 accggaagca gaagcattaa aggctcttac cgtttatccc gcacaattgt taggtgttga
     1081 caaacaactt ggcac
//
LOCUS       DAOMYG010000607         1092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_55311_length_1092_cov_6.469624, whole genome shotgun sequence.
ACCESSION   DAOMYG010000607 DAOMYG010000000
VERSION     DAOMYG010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55311_length_1092_cov_6.469624"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1092
                     /locus_tag="PK610_14125"
     CDS             <1..>1092
                     /locus_tag="PK610_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ37129.1"
                     /translation="IKLYGDKATFNNEIRLSNNGLQADGTIKFLTTTADSKAFTLFPD
                     SLSGIAEKYKNVEQKGSVEIPESHGSQLMIRYHPKAKVLYTQSRDSAIHLFDNTCKMR
                     GTTALRPDGMTGKGRIYFNTAELTSRKFKFKTKVIDSDTANFKLKNHDLTQELAFKTD
                     NVNAHIDFVQRLGEFKSNGNETKVEFPENEYICFMDRFKWFMDNEDIELESDKRSVSG
                     NINIDTDIDLSGSNFFSVRQGQDSLNFAAPKAKFDLKKKVITCQKVDFVGVGDARIYP
                     DSNKVLIRRKAEMDPLKNAMIVCNYVTKYHKITEVTAKISGRKEYKASGKYAYIDENK
                     MEQKIYFANISLDTTFQTYAKGDVKPEER"
BASE COUNT          386 a          206 c          226 g          274 t
ORIGIN      
        1 aatcaaattg tatggcgata aagccacctt caataatgaa atacgacttt ccaataacgg
       61 tttgcaggca gatggaacaa ttaaatttct tacaaccact gccgattcaa aagcatttac
      121 actattcccg gattcgcttt ctggaatagc agaaaagtat aagaacgtag aacaaaaggg
      181 aagtgtagaa atccccgaat cgcatggatc gcaattgatg atacgctacc atccaaaggc
      241 aaaggtgttg tacactcaat cgcgcgactc agcaattcat ctttttgata atacctgcaa
      301 aatgcgggga acaacagccc ttcgtccgga tggaatgacg ggcaagggga ggatttactt
      361 taatacagcg gaactcacct cccgcaaatt caagtttaaa acaaaagtga ttgattcgga
      421 tactgccaac ttcaagctga agaaccacga tttaacacaa gagcttgcat ttaaaaccga
      481 taacgtaaat gcccatattg attttgttca gcgattgggc gaattcaagt cgaacggaaa
      541 tgaaacaaaa gtggaattcc ccgagaatga atacatctgt ttcatggacc gattcaaatg
      601 gttcatggac aatgaagata ttgaacttga atccgacaaa cgttctgttt ccgggaatat
      661 taacatcgat acggatatcg atctgtcggg ttcaaatttc ttctcggtgc gacaaggaca
      721 ggattcgttg aattttgctg caccaaaagc gaaattcgat ttgaagaaaa aagtaattac
      781 atgtcagaaa gttgattttg taggtgtggg tgatgcgaga atctatccgg atagcaataa
      841 agtactgatc cgcagaaaag cggaaatgga tcccttaaaa aatgcaatga tcgtttgtaa
      901 ctatgttacg aaataccata aaatcactga ggtaacagcc aaaatcagcg gacggaaaga
      961 atataaggct tcaggaaaat atgcctatat cgatgaaaat aaaatggagc aaaaaatcta
     1021 tttcgccaat atttcgctcg atacaacatt tcagacttat gcaaaaggtg atgttaaacc
     1081 cgaagagcga tt
//
LOCUS       DAOMYG010000608         1078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_56473_length_1078_cov_12.223851, whole genome shotgun
            sequence.
ACCESSION   DAOMYG010000608 DAOMYG010000000
VERSION     DAOMYG010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56473_length_1078_cov_12.223851"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1028
                     /locus_tag="PK610_14130"
     CDS             102..1028
                     /locus_tag="PK610_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRJ37130.1"
                     /translation="MSLLIVGTVAFDAIETPFGKTDKIVGGAATYIALAASYFNKKNK
                     LISVIGDDYDMDMLEKLKGKGIDLTGLQIKKGEKSFFWAGKYHYDLNSRDTLDTQLNV
                     LATFDPQVPESYKDCEFLMLGNLDPKVQMRVIEQLPNRPKLVVLDTMNFWMDIALDSL
                     KEVMKKVDVITINDEEARQLSGEYSLVKAARKILTMGPKYVVIKKGEHGALLFDKEHM
                     FFAPALPLEEVMDPTGAGDTFAGGFIGFIAHTKDISFENMKRAIIIGSSLASFTVEKF
                     GTERILDLKESEIDARIQSFVELVDFNIEFVE"
BASE COUNT          320 a          208 c          243 g          307 t
ORIGIN      
        1 aaaaccaaac acccccatgt tccggaaagt cagttataat cagccagata gggcaggctt
       61 gaattccgat cattctctta tctttgcaaa aattactttt tatgagtctt cttattgttg
      121 gaaccgttgc gttcgatgct attgaaaccc cttttggaaa aaccgataaa attgttggtg
      181 gagcagcaac ttatattgct ttggccgctt cttattttaa taagaaaaac aaactgatca
      241 gtgttatcgg cgacgattac gatatggata tgctcgagaa gctgaaagga aaaggaattg
      301 atcttaccgg actccagatc aaaaaaggag agaagtcatt tttctgggct ggtaagtacc
      361 attatgatct taactcacgc gatacgctgg atacgcagtt aaatgtactt gcaacattcg
      421 atccgcaggt tccggaatct tataaagatt gtgaattcct gatgcttgga aaccttgatc
      481 ccaaagttca gatgcgtgtc atcgagcaat tgcctaatcg tccgaagctg gttgtgctcg
      541 acaccatgaa tttctggatg gatattgcat tggacagtct taaggaagtg atgaagaaag
      601 tagacgttat caccattaac gatgaagaag cgcgtcagct atccggagaa tattcgctgg
      661 tgaaagcagc acgtaagatc cttaccatgg gaccaaaata cgttgtgatt aaaaaaggag
      721 aacacggagc cctgttgttt gataaggagc acatgttctt tgcgccagca cttccgttgg
      781 aagaagtaat ggatcccacc ggagcgggcg atacatttgc tggtggattc attggcttta
      841 ttgcacacac caaggatatc tctttcgaga atatgaagcg tgcaattatc ataggctcat
      901 cgcttgcttc ctttacagtg gagaaattcg gtacagaacg tattctcgat cttaaagaat
      961 cagaaatcga tgcgcgcatt caaagtttcg ttgagcttgt cgatttcaat attgaatttg
     1021 tagagtaagc gactaacgtt tcaacggttc aaaaatttca ggaaattctt gcgatgcg
//
LOCUS       DAOMYG010000609         1039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_5632
            NODE_59757_length_1039_cov_6.048780, whole genome shotgun sequence.
ACCESSION   DAOMYG010000609 DAOMYG010000000
VERSION     DAOMYG010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867853
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.33
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,810
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 18
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59757_length_1039_cov_6.048780"
                     /isolate="AMR_MDS_5632"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PK610_14135"
     CDS             <1..492
                     /locus_tag="PK610_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HRJ37131.1"
                     /translation="SEVVKIGFKYIADIMVDQDILLGGEESGGIAITGHIPERDGIWM
                     GLVIWEFMAKSGKTLDELIDEVYAITGKFAFERNDLHLKEDLKQRIVAKIQSGGWEKF
                     GPYTVRNVESIDGTKYYFDNNEWVMIRPSGTEPVLRVYAESSDQAGAFRILEEVKKVI
                     LAC"
     gene            complement(604..>1039)
                     /locus_tag="PK610_14140"
     CDS             complement(604..>1039)
                     /locus_tag="PK610_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation/H(+) antiporter"
                     /protein_id="HRJ37132.1"
                     /translation="RLFENSPFDERTRNIIGKSKVPVGVLIDKNFSKADAVFIPVFSE
                     SDTFLIQFAQKLINNSGSQITILDVSGQIKNNPELKEKIRAIEQKAPNHINVLNERII
                     DKEFLIGQHLMVVSIESWKKLVDTKSLWLSDIPSTLIVKEGN"
BASE COUNT          305 a          187 c          247 g          300 t
ORIGIN      
        1 tcggaagtgg tgaaaattgg tttcaagtac attgccgaca tcatggtaga tcaggatatt
       61 ctacttggag gggaagaatc gggaggaatt gcaattaccg gacacatacc cgagcgcgac
      121 ggtatttgga tgggattggt catttgggaa ttcatggcaa agagtgggaa aacgctcgat
      181 gagctcattg acgaggtgta tgctattacc ggaaaatttg cttttgaacg caatgatctg
      241 catttgaaag aagacctcaa acaacgcatc gttgcaaaaa tccaaagcgg tggttgggaa
      301 aaatttggac cgtataccgt tcgcaatgtt gaatcgattg acggaacaaa atactatttc
      361 gataataacg aatgggtgat gattcgtccg agcggcacag agcctgtttt acgggtctat
      421 gccgaatctt cagaccaggc gggggcgttc aggatactgg aagaggtgaa gaaggtgatt
      481 ttggcgtgtt gagaaaactg aagttaggag aagggcgaag aaagaagacg tggactaaat
      541 ccatttacgt tgtagcaaag atggacgagt cctatttacg cataattgca gcgcgaggca
      601 acctcaattt ccttctttca ctataagtgt ggatggaata tccgataacc agaggctttt
      661 tgtatcaact aattttttcc agctttcgat gctgacaacc atcaaatgtt gtccaatcaa
      721 aaattcctta tcgatgatgc gttcattgag aacatttata tgattggggg ctttttgctc
      781 aatggctctt attttttcct tgagttcagg attgtttttg atttgtccgc ttacatcaag
      841 aattgttatc tgcgaacctg aattgtttat gagtttctgt gcaaactgaa tcaaaaacgt
      901 atccgactct gaaaataccg gaatgaaaac tgcatcggct ttcgaaaaat tcttgtctat
      961 aagcacacca acaggtactt tactctttcc aataatatta cgcgttcttt cgtcgaaagg
     1021 tgaattctca aacagacgt
//
